LOCUS       DASRZS010000001         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1924483, whole genome shotgun sequence.
ACCESSION   DASRZS010000001 DASRZS010000000
VERSION     DASRZS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924483"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFH83_00005"
     CDS             complement(<1..387)
                     /locus_tag="VFH83_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484765.1"
                     /translation="MIERLSPRERVSLALRHQETDRVPIAMVCSGINPAARAELERFL
                     GQRRGLTVEQYLKPLIDVVPVGPRYVGPTLPENTDIWGVRRRAVSYGLGSYDEIDHYP
                     LAGAQSVADLDAHDWPRIDWWDYESIR"
     gene            complement(384..1385)
                     /locus_tag="VFH83_00010"
     CDS             complement(384..1385)
                     /locus_tag="VFH83_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HET6484766.1"
                     /translation="MEQRIVFNGPKRAELEAFSVPAVKDGEVEVRVSVSLMSTGTEGI
                     IYNRWFDPGTHWDNWIKYPFHPGYSAVGTVAAAGSGVSSPKVGDRVAIRKSHASAHVL
                     PAAQCIVVPPVVSDEQAAWFALAKIAAMGARVAGHGLGSSVLVIGAGPIGQMAIRWAF
                     VAGAEAIIVVDPVGPRLELARVGGATATIDKPIAEAAPLILQANHGSNPRVVIDSTGN
                     AEVFAVALSVVERFGTVVVLGDTGAPAQQRLSPDLITKGLRVVGAHDTHETAEWNAGI
                     IARLFFQAVSDGRFRFGSLVSHTFPPQEAGAAYELATHHRKDTMGILFDWKGRGRGK"
     gene            complement(1419..>2139)
                     /locus_tag="VFH83_00015"
     CDS             complement(1419..>2139)
                     /locus_tag="VFH83_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6484767.1"
                     /translation="DQVFRLPPRIIAFPLRAQNFAEAWSKFLPFNRFLVNTLLIAVLS
                     TIGNLLSSSLVSFSFARLSWPGRDFFFIVILASLMLPYQVTLIPVFILFKTIGWVNTY
                     LPIIIPTFFGNAFFIFFMRQFITSIPFELDSAARIDGASTFTIFLRILMPLCTAPLAT
                     VAIFSFIWNWDELVRPLIYLNQEKLYTITLGMTMFKNQSIVYWNYLMALAILAMIPPL
                     VIFALAQKWFTQGITITGLKG"
BASE COUNT          403 a          677 c          720 g          339 t
ORIGIN      
        1 ccggatggac tcgtagtccc accagtcgat gcgcggccag tcgtgcgcat ccaggtccgc
       61 cacggactgc gcgccggcca acgggtagtg atcgatctcg tcgtaggacc ccaggccgta
      121 gctgaccgcc cggcgcctca cgccccagat gtccgtgttc tccggcaggg tgggcccgac
      181 gtaccgaggc cccaccggca ccacgtcgat gagaggcttc aggtactgct ccaccgtgag
      241 gccgcgccgc tggcccagga aacgctccag ctctgcccgc gccgcggggt tgatccccga
      301 gcagaccatg gcgatgggca cccggtccgt ctcctggtgg cgcagggcca gggaaaccct
      361 ctctcgaggg gaaaggcgtt cgatcatttc ccgcgacccc gccccttcca gtcgaagaga
      421 atccccatgg tatccttgcg gtggtgcgtt gctagctcgt aggcggcacc ggcctcctgc
      481 ggcgggaatg tgtgggagac gagactcccg aaccgaaacc gcccgtccga gaccgcctgg
      541 aagaagagcc tggcaatgat gcccgcgttc cactccgcgg tctcgtgggt gtcgtgggcc
      601 cccaccaccc gcagcccctt ggtgatcagg tccggggaga gtcgctgctg ggcgggggca
      661 ccggtatctc ccaggacaac aacggtgccg aatcgctcca cgacggagag cgcgaccgcg
      721 aacacctcgg cgttcccggt cgagtcgatg actacgcgcg gattgctccc gtgattggcc
      781 tggaggatga ggggggcggc ctccgctatg ggcttgtcga tcgtggcggt cgcgccgcca
      841 acccgggcga gctccagccg cgggccgacc ggatccacca cgatgatggc ctccgcgccc
      901 gccacgaatg cccagcggat ggccatctgg ccgatcggtc ccgcgccgat caccaggacg
      961 ctggaaccca gcccgtggcc ggcaacgcgc gcgcccatgg cggcgatctt ggcaagggcg
     1021 aaccaggcgg cctgctcgtc cgagacgacg ggcgggacca caatgcactg ggcggcgggc
     1081 aggacgtgcg cgctggcgtg gctcttgcgg atcgccaccc ggtccccgac cttgggagag
     1141 gacacgccgg agcccgccgc ggccacggtc ccgacggccg aatagccagg gtggaacggg
     1201 tatttgatcc agttgtccca gtgggttccc ggatcgaacc agcggttgta gatgatccct
     1261 tccgtgccgg tgctcatcag ggacacgctg accctgacct ctacctcacc gtccttcacc
     1321 gccgggaccg agaaagcctc cagctccgcc ctcttgggtc cgttgaacac gatccgttgc
     1381 tccatgggtt tccctcctga ggctgggtcg gcggggtgct accccttcag ccccgtgatc
     1441 gttattccct gcgtgaacca cttctgggcc agggcgaaaa tgaccagcgg cgggatcata
     1501 gccagaatgg cgagggccat gaggtagttc caataaacga tcgactggtt cttgaacatc
     1561 gtcatcccga gcgtgatcgt atagagcttc tcctggttga ggtagatgag cggacggacc
     1621 agctcatccc agttccagat gaagctgaag atggccaccg tggccagcgg cgccgtgcac
     1681 aggggcatca ggatccgaag gaagatcgtg aaggtcgagg cgccgtcgat gcgggccgcg
     1741 gagtccagct cgaacggtat gcttgtgatg aactgccgca tgaagaagat gaagaacgcg
     1801 ttcccgaaga acgtggggat gatgatcggc agataggtgt tcacccagcc gatggtcttg
     1861 aacaggatga acacggggat gagcgtgacc tggtagggca gcatcaggga ggccaggatc
     1921 acgatgaaga aaaagtccct ccccggccag ctcaaccggg caaaggaaaa ggacaccagg
     1981 gagctggaga ggaggttccc gatggtggag agcaccgcga tgagaagcgt gttcaccagg
     2041 aaccggttga agggaaggaa cttggaccag gcctcggcga agttctgagc ccgaagcgga
     2101 aacgcgatga tgcgcggcgg gagacggaac acctggtcg
//
LOCUS       DASRZS010000002         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3079059, whole genome shotgun sequence.
ACCESSION   DASRZS010000002 DASRZS010000000
VERSION     DASRZS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3079059"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..854
                     /locus_tag="VFH83_00020"
     CDS             159..854
                     /locus_tag="VFH83_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HET6484768.1"
                     /translation="MRRFPHPVMASFIALAMVLGAGNPAVGQAANSSVTRGQWILRLS
                     PGPQYASSTRFLLIFKIPLYPQVAAWLEDANGNFIDTIYVTKKAAKRSFYSAPASGRP
                     EALPVWYHRQPEQQRSVDAVTGATSIGPFEFHSTVGESLSPGRYVVFLEINSSYDYND
                     RYTRSNSGVNGQPSLIYRAEIQVGRGPSTAVFVPIGTGSVDGSDGLIHSGLEGITTAL
                     RLLTRAEIEYRGD"
     gene            917..1396
                     /locus_tag="VFH83_00025"
     CDS             917..1396
                     /locus_tag="VFH83_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6484769.1"
                     /translation="MEVYFLRHGIALDRTEWHQDDRQRPLTEEGIARMTREAKTLNRL
                     GLGLDAIITSPLTRARHTAEIVAAELGLQDKLEEDERLGLRFALPELREVLTAHAAVK
                     AIMLVGHEPSFSDTISRLIGGARLECKKGGLARVDISGPVAPEGILQWLLPPRVLAP"
     gene            complement(1444..>2081)
                     /locus_tag="VFH83_00030"
     CDS             complement(1444..>2081)
                     /locus_tag="VFH83_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6484770.1"
                     /translation="RITIEDQGTGIAPTVLPHIFDPYFTTKAKGTGLGLSTVFSIVRR
                     HDGHTGVQSSVGVGTCFTIHLPARPDVHGSGPSGFEPAPRGQGRLLIMDDEPSVRALL
                     ERILTHLGYEVCSTADGAEAVERYREARQAGKGYAAVILDLTIPGGMGGAEAIRLLRE
                     YDPEVKAVVSSGYSTGAVMAEYRSWGFQGMIAKPYTVQQLARLLQQVLAEP"
BASE COUNT          367 a          668 c          651 g          395 t
ORIGIN      
        1 aacgcgttcg atctaggact gccgccggag atccctctgt tctcctttcc ggtaaccctt
       61 gttcttatcg gcgttggcgg cgttcttgtg atcgtctgga acgtcttcct ttgcgttacg
      121 ttgttgcagc ggtcggcgag ctcaggggga gaacgacgat gaggagattc ccgcacccgg
      181 tcatggcttc tttcatagca ctggcgatgg tcctgggagc aggcaatcct gccgtggggc
      241 aggccgcaaa ctcctccgtg acccgcggtc agtggatcct gcgtctctca ccggggccgc
      301 aatacgcatc cagcacgcgg tttctgctca tcttcaagat tcctctctat ccccaggttg
      361 ccgcctggct ggaagacgcc aacggcaact tcatcgatac gatctacgtc accaagaagg
      421 ccgccaagcg ctccttttac tcggcccctg cctccggaag acccgaagcg cttcccgtct
      481 ggtatcaccg acaacccgag caacaacgat cggttgacgc agtgacggga gcgacctcga
      541 tcggtccctt cgagttccac tccacggtcg gcgagagcct ctctccggga cgctacgtcg
      601 tgttcctcga gatcaacagc tcctatgact acaacgatcg gtatacgcgt tcgaactccg
      661 gagtcaatgg ccagccgtcc ctgatctacc gcgccgagat ccaggttggc cggggacctt
      721 ccaccgctgt cttcgtcccc atcggcaccg gttccgtgga cggctccgac gggctgatcc
      781 attcaggcct ggaagggatc acgaccgcct tgcgcctcct gacgagagcg gagatcgaat
      841 accgcgggga ctgaccccat cagcgggaac gcgatccacg gttgaccacg ctgcgggttg
      901 gtcgcactat ccgtccatgg aagtttattt tctgcgccac ggaatagcgt tggaccggac
      961 ggagtggcac caggatgacc gtcaacggcc actgaccgag gaagggatcg cgcgcatgac
     1021 ccgagaggcg aagaccctga accgattggg gctggggctc gacgccatca tcaccagccc
     1081 gctcacgcgc gctcgccaca cggccgagat cgtcgctgcg gagctcggac tccaggacaa
     1141 gctggaggag gacgagcggc tgggtctcag gttcgcgttg ccggagctga gggaggtgct
     1201 cacagcgcat gcagcggtga aggcgatcat gctcgtgggt cacgaaccgt cgttcagtga
     1261 cacgatcagt cgactgatcg gcggtgcgcg gctggaatgc aagaagggcg gtctggcccg
     1321 ggtggacatt tctggtccag tggcgccgga gggcattctc cagtggctgc tgccgccgag
     1381 ggtgctggcc ccctagttcc aggaggccgg cgccccgact cgtgtcggtg aggccgagga
     1441 agtctatggc tcggccagga cctgctggag caaccgagcc agttgttgca cggtgtatgg
     1501 tttggcgatc atgccttgaa agccccacga gcggtactcg gccatgaccg cgccggtcga
     1561 atagccgctt gagacaacgg ccttgacctc tggatcgtat tcccgaagaa ggcggatggc
     1621 ctcggcgccc cccatccccc cggggatggt gaggtccagg atcacggccg cgtagccttt
     1681 ccccgcctgg cgcgcctcgc ggtatcgttc gaccgcctcc gctccatcgg ccgtcgagca
     1741 cacctcgtat cccagatgcg tcaggattcg ctcgagcaga gcgcgaacgg agggctcatc
     1801 gtccatgatc agcagtcgtc cctgtcccct tggcgccggc tcgaatcccg aaggacccga
     1861 cccgtgaaca tccggccggg ccggcaggtg gatcgtgaag caggttccga ctccgacgct
     1921 ggactggacg ccggtgtgcc cgtcgtgacg ccgcacgatt gagaacaccg tggacagacc
     1981 cagcccggtc cccttggcct tggtcgtgaa gtacggatcg aagatatggg gaagcaccgt
     2041 cggcgcgatg ccggttccct gatcttctat ggtgatccgc a
//
LOCUS       DASRZS010000003         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1541141, whole genome shotgun sequence.
ACCESSION   DASRZS010000003 DASRZS010000000
VERSION     DASRZS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541141"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VFH83_00035"
     CDS             <1..611
                     /locus_tag="VFH83_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6484771.1"
                     /translation="QYLTRTEERYAEPDWGGARYVMSPPLRDQASREALWSGIRDGEV
                     QTIGTDHCPLSFQGQKDINGKDDYRRIPNGAPGIETMLMLLHSEGVRKGRLSLEKMVE
                     VVSTGTARLFGLSRKGEIAAGKDADIVVFDPRRAFTIRQASLHQNVDYTPWEGWEITG
                     MPEIVYSRGSRVAQWAGDRMEFVGQAGWGRFVRREPPFQPQA"
     gene            complement(619..1440)
                     /locus_tag="VFH83_00040"
     CDS             complement(619..1440)
                     /locus_tag="VFH83_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484772.1"
                     /translation="MRRWSFVGGAILIVLGLFALFQVGLDALGVHFRIWWIFWPLVLI
                     GIGAWIVTGVSRRGIPGDFTREDASVPLDGAGEASVTVRHGAGRLTIGAGAGGGQLLS
                     GSFGGGLDASRRMDGGRLVVDMRMRDRGFSRYWVPWSHGWSNLRDWDFTMNPDVPVAL
                     NLETGASETRLNLTDLKVTELRLSTGASATYIDLPARAGYTRVSIESGAASVKLRVPN
                     GVAAKVQVRSALAGIHVDRSRFAATGGGYQSSDWDGAQNKLEILIETGVGSVDVY"
     gene            complement(1442..1951)
                     /locus_tag="VFH83_00045"
     CDS             complement(1442..1951)
                     /locus_tag="VFH83_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484773.1"
                     /translation="MDHRSRSALVTGTILILLGAFFLVTRIVPGFLETFSWPIYVVGV
                     GVVFLVLALATWTPGLAVPACIIGGIGALLYWQNQTGAWWTWSYAWALIPGFVGVGVA
                     VSELLEGRPLRALTEGGWPILISLLLFFLFGSFLGGLPWFGPWWAVVLIAIGVLIVLR
                     PLARRRKGD"
     gene            2115..>2445
                     /locus_tag="VFH83_00050"
     CDS             2115..>2445
                     /locus_tag="VFH83_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET6484774.1"
                     /translation="MGITQAIATFGTHVIGSIGYLGVFLLMVSESMVLPTPSEAVMPF
                     AGFLVAEGTLSAVGVVVFATLGSIVGSLASYAIGLYGGRPFVERFGKYLLLDRRDLEM
                     TDRFFHRR"
BASE COUNT          428 a          814 c          845 g          358 t
ORIGIN      
        1 cccagtatct gacccggacc gaggagcgat acgcagagcc ggactggggc ggcgcccggt
       61 acgtcatgtc gccgccgctg cgggaccagg cgagcagaga agccctctgg agcgggattc
      121 gggacgggga agtgcagacg atcggcaccg accactgccc cctgagcttc cagggtcaga
      181 aggacatcaa cggcaaggac gattatcgac ggatccccaa cggcgctcca gggatcgaga
      241 ccatgctcat gctcctccat tcggagggcg tccgaaaggg gaggctgtcc ctggaaaaga
      301 tggtggaggt cgtctccacg ggcaccgcgc gtctgttcgg cctgtcgcgg aagggggaga
      361 tcgccgcggg caaggatgcg gacatcgtcg tcttcgatcc gcggcgcgcc ttcacgatac
      421 gccaggccag cctgcaccag aacgtggact acacgccgtg ggagggatgg gagatcacgg
      481 ggatgccgga gattgtctac agcaggggat ctcgcgtcgc ccagtgggcg ggcgatcgga
      541 tggagttcgt ggggcaggcc ggctggggcc ggttcgtgcg gcgggagccg ccgttccagc
      601 cccaggcctg acgccagatc aatacacgtc taccgagccg actcccgttt cgatcaggat
      661 ctccagcttg ttctgcgcgc cgtcccagtc ggaggactgg tagccgccgc cggtggccgc
      721 gaaccgggac cggtcaacgt ggatgcccgc cagggcgctt ctcacctgaa ccttcgccgc
      781 cacgccgttg gggacacgca gcttcaccga agccgcgccg gactcgatgc tcacccgggt
      841 gtagccggcc cgggccggaa ggtcgatgta ggtggcgctg gcgcccgtgc tgagccgaag
      901 ctccgtgacc ttgaggtccg tcaggttgag ccgggtctcg ctggcgcccg tctccaggtt
      961 gagcgccacc ggcacgtcgg gattcatcgt gaagtcccag tcccggaggt tggaccagcc
     1021 gtggctccag gggacccagt accgcgagaa gccgcgatcc cgcatccgca tgtccacgac
     1081 gagccgtccg ccgtccatgc gccgggaggc gtccagcccg ccgccgaagc tgccggacag
     1141 gagctgcccg ccgccggcgc ccgcgccgat ggtgagccgg cccgccccgt ggcgcaccgt
     1201 cacgctggct tcccctgcgc cgtccagcgg cacggaggcg tcttcccggg tgaagtcgcc
     1261 ggggatccct cttcgggaga ctcccgtcac gatccaggct ccgatcccga tcagcaccag
     1321 gggccagaag atccaccaga tccggaagtg aacgcccagc gcgtccagtc ccacctggaa
     1381 gagggcgaac agaccgagca cgatgaggat tgcgccgcca acgaagctcc atcgccgcat
     1441 gtcagtcccc ctttcgcctt cgagcgagag gcctgaggac gatcagcaca ccgatggcga
     1501 tgagcacgac ggcccaccag ggcccgaacc agggaagccc gcccaggaac gagccgaaga
     1561 ggaagaacag gagcaggctg atcaggatgg gccagccccc ctccgtcagc gcccgcaggg
     1621 gacgtccttc caggagctcg ctcaccgcca cgccgacccc cacgaaaccg ggaatcagcg
     1681 cccacgcgta ggaccacgtc caccatgcgc cggtctggtt ctgccagtac aggagggcgc
     1741 cgatcccgcc gatgatgcac gccggcaccg ccagcccggg ggtccaggtg gccagggcca
     1801 ggaccaggaa gactacgccg accccgacga cgtagatcgg ccaggagaac gtttccagga
     1861 agcctgggac gatgcgagtc accaggaaga acgctccgag cagaatcagg atggtgccgg
     1921 ttaccagggc ggatcgagac cgatggtcca ttccagcctc ctttcggtgt ttcagtatag
     1981 acaggccggc acagacctgt cgagggattc aacaccgcgt gaggccgcgg cggtcaccac
     2041 gtccgccggg tcgccgtgcg gtggctatgg atgcgcggcg tccgcggagg tacagtcacg
     2101 ttcgggagga catcgtgggg atcacgcagg caatcgcgac gttcgggacg cacgtcatcg
     2161 gcagcatcgg gtacctgggc gtgttcctgc tcatggtctc ggaaagcatg gtgctgccca
     2221 cgcccagtga ggcggtgatg ccgttcgccg ggttcctggt ggcggaaggg accctgtccg
     2281 cggtgggcgt cgtggtcttc gccaccctgg ggagcatcgt gggctcgctg gcctcctacg
     2341 ccatcggcct gtacgggggg cggcccttcg tggaacggtt cgggaagtac ctgcttctgg
     2401 accgtcggga cctggagatg acggaccggt tcttccatcg ccgcg
//
LOCUS       DASRZS010000004         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_387158, whole genome shotgun sequence.
ACCESSION   DASRZS010000004 DASRZS010000000
VERSION     DASRZS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387158"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VFH83_00055"
     CDS             complement(<1..1041)
                     /locus_tag="VFH83_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HET6484775.1"
                     /translation="MSQNTHADHPYIGRQAVSTAEQTAEALASLNADRSPGGRDAVEV
                     VYYFELEHQGRALDGLANAAKMVLEHGTLKPWHAEGDASVRKPDDYDQFMSWATDIQL
                     LDSGPGWESGLVGIAYPLAFFDKRSDGRVPLSQLLMAMASEPFSAFSFYRSAKIIDVR
                     FPPSLRSRFPGLRWGHDRVRAYLGLAADEPIIGTIVKPKTGLTPELFSRSVVEAARAG
                     ARFTKADENMHLSLKDVTRYVRQVCRDLRAAGFDLGRSEHPTGRRFLFAPHITTDADS
                     LDDYAQAALEAGANALMFSPYWSGGFLPMSRIVEKYDVPVYAHTAGMNVMSGSPFWGI
                     DSRVHYVMAALFG"
     gene            1119..1772
                     /locus_tag="VFH83_00060"
     CDS             1119..1772
                     /locus_tag="VFH83_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET6484776.1"
                     /translation="MQRKAILFDLDGTLLDTLEDLGDSMNAVLSARGLPTHPLESYRY
                     FVGDGVGELVRRALPADRAADETLVHACTEGMRLEYDKRWHDKTRLYDGVGSLLDALT
                     RAGVKLTVLSNKPDAFVRLIVQHYFAAWRFAAALGARPSVPRKPDPAAAIEISRLLRL
                     APSEFLYVGDTNTDMQTARAAGMFAVGALWGFRTQQELEESGAAAVAAHPMDVVSLL"
     gene            complement(1782..2156)
                     /locus_tag="VFH83_00065"
     CDS             complement(1782..2156)
                     /locus_tag="VFH83_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HET6484777.1"
                     /translation="MKEIVKTDGAPAPVGPYSQGVVAGKLLFVAGQTPKDPRTGQIPQ
                     DFKAQVEQCLRNIQAIVTAAGATLADVVKVNAYLKDLGQFKAFNEVYERVFQAPYPAR
                     TTVGADLPGIAVEIDAIAVLPG"
BASE COUNT          377 a          740 c          774 g          427 t
ORIGIN      
        1 cccaaagagg gcggccatga cgtagtgcac ccgcgagtcg attccccaga acggagaccc
       61 gctcatgacg ttcatcccgg ccgtgtgggc gtacaccggc acgtcgtact tctcgacgat
      121 cctcgacatg ggcagaaagc cgccgctcca gtatggggag aacatcaggg cgttggcgcc
      181 cgcctccagg gcagcctgcg cgtagtcgtc cagggagtcg gcgtccgtgg tgatgtgtgg
      241 ggcgaagagg aaccgccggc cggtggggtg ctcgcttcgt cccaggtcga agcctgcggc
      301 gcggaggtct cggcaaacct ggcggacgta tcgcgtcacg tccttcaggc tgagatgcat
      361 gttctcgtcc gccttggtga accgggcgcc tgcccgggcc gcctcaacga cgctgcggga
      421 gaagagctcc ggtgtgagcc cggtcttggg tttgacgatg gtgccgatga tcggttcatc
      481 ggcggccagc cccaggtagg cacgcacccg gtcgtgcccc caacgcaatc cggggaaccg
      541 agacctcagg gacggcggga accgtacgtc aatgatcttg gccgagcggt agaacgagaa
      601 cgcggagaac ggctcgctcg ccatggccat caagagctgg ctcaggggca cgcgaccgtc
      661 cgaacgcttg tcgaagaagg cgagggggta ggcgatgccg accagtcccg attcccagcc
      721 gggccccgag tccaggagct ggatgtcggt tgcccagctc atgaactggt cgtagtcgtc
      781 gggcttccgt acgctcgcgt caccctccgc gtgccacggt ttcagagtcc cgtgctccag
      841 gaccatcttt gcagcgtttg ccagaccgtc cagggcccgc ccctggtgtt ccaattcgaa
      901 gtagtagacc acctccaccg catcccgacc gccggggctt cggtccgcgt tcaggctggc
      961 gagcgcctcc gcggtctgtt ccgccgtgga gacagcctgc cttccgatat agggatggtc
     1021 cgcgtgtgtg ttctggctca tcgtgtctca gcgcgaatag tgtcgagaag ccgcgggggt
     1081 gtcaattctg gagccggacc ggctatactt cgaccgctat gcaacggaag gccattctgt
     1141 tcgatctgga cgggacgctc ctggataccc tggaggacct cggagactcc atgaacgcag
     1201 tgctgtctgc ccgcgggctc cccacccatc cgctggaatc ctatcgatac ttcgtggggg
     1261 acggtgtggg agagctggtc cgccgcgccc taccggccga ccgggccgcg gacgaaacgc
     1321 tcgtccacgc gtgcacagaa gggatgcgcc tggagtacga caaacggtgg cacgacaaga
     1381 cccgcctcta cgatggggtc ggatcgctcc tggacgcgct gacccgcgcg ggggtgaagc
     1441 ttaccgtgct ctccaacaag ccggacgcgt tcgtgcgtct gatcgtgcag cactacttcg
     1501 cggcatggag gttcgcggca gccttggggg cgcggccttc ggtgccgagg aagccggatc
     1561 ccgcggcggc catcgagatc agccggcttc tgaggctggc cccttcggag ttcctctacg
     1621 tcggagacac caataccgat atgcagaccg cccgggcggc gggcatgttc gctgtgggcg
     1681 ccctctgggg gttcagaact cagcaggagc tggaggaatc cggggcggcc gccgtcgcgg
     1741 ctcaccctat ggacgtcgtg tctctgctgt gagccacggg gtcaaccggg cagcacggct
     1801 atcgcgtcga tctccactgc gattcccggc agatcggcgc cgaccgtggt ccgtgccggg
     1861 tagggtgcct ggaagaccct ctcgtacacc tcgttgaatg ccttgaactg acccaggtct
     1921 ttcaggtacg cgttcacctt caccacgtcg gcaagggtag ccccggccgc ggtcacgatt
     1981 gcctggatgt tccgtagaca ctgctccacc tgggctttga agtcctgtgg gatctgcccg
     2041 gttctggggt ccttgggtgt ctgaccagcg acgaagagca gcttccctgc aacgacaccc
     2101 tgggaatacg gccccaccgg cgcgggtgcc ccatcggtct tgacgatctc tttcatggcg
     2161 actctatgct gtaccgggga agggcccggt ggtcaattgc tgatcgactc gcggggctca
     2221 gaagggacca ctacgagcgg gacgcgctcc gagtgtcgga tgaccgtcat ccgggttgcc
     2281 ttcccgatcc gctcgccggt cagggttttg tgcaggtc
//
LOCUS       DASRZS010000005         6097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2697160, whole genome shotgun sequence.
ACCESSION   DASRZS010000005 DASRZS010000000
VERSION     DASRZS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2697160"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1503
                     /locus_tag="VFH83_00070"
     CDS             220..1503
                     /locus_tag="VFH83_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET6484778.1"
                     /translation="MSYLMACVEGFPDRRKGRNIQFSLHDVTRGAFGVFFCQSPSFLA
                     FQELMQQQQGKNNARTLFGVEQIPSDNHIRALLDPVDPARLNAVYQKCFELMQQHGLV
                     HPMRAVGNSLLIAMDATGYFLSESIHCDSCMVSKHRDGRVTYSHAAVLPAVVSPQHSH
                     VIPLQPEFLRRQDGHERQDCEFQAALRWIKDHGAQLSPLGVTLLGDDLYSRTPLIREV
                     QQQELDFIFVAKAVMHKHLQEEVEGLEKLGGVQQLQQIAWTGRHHRHYHYRWVREVSL
                     TAEKDSPVVSWVELQILDEAGKITFRNSWVTSHPINEQTVVELVRAARSRWKIENEDF
                     NTLKTKGYHFEHNFGHGQQYLSQTLLSLNVIAFLFHTLLELMDKRCALLRSTLPRRDT
                     FFQHVATLTQYLCFDGWAALMLFMLNGLHLPDPDG"
     gene            complement(1796..3940)
                     /locus_tag="VFH83_00075"
     CDS             complement(1796..3940)
                     /locus_tag="VFH83_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HET6484779.1"
                     /translation="MFHRSRGKCKPLAHIAAGRGLTAWVCLALLAAAGSTSAFGEPLR
                     VGPVRAELLSEASAIAAGQTFTVGVHLVSNPDWHTYWKYPGDSGLPTTLEWHLPPGFA
                     AGPIQWPVPQRIETLGDVTYGYPGDVMLLVDITPPARLWQKQATLAVTVSWLACRIEC
                     TPGKAELTASVPIGTGAPAPDPSLIGAFRRARQALPSSAPAGMLTATSTPSAVQLRIL
                     GLQAAAAQVAFYPASAGQIDLKAPQTTGRTGPDRVLTLRRAAGTGQRLTGILVVSDGG
                     STRALDVDLPVSAAPAAGLGILLALVLAFIGGVILNLMPCVLPVVSLKVLGLVRHAPD
                     GHSSAIGQGLLFAAGVLVSFWAIAAVLLALRAGGQSLGWGFQFQNPVVVAIAAALFFL
                     IGASLFGLFELQAFAPRASAPRAFAPRAISGPRTAGSFLSGLFATAVATPCTAPFMGA
                     ALGYALGQPLPVAFGVFTALGIGMAAPYVVLSAAPGLSSRLPKPGPWMETLKQILGFP
                     MMAAVIWMLFVLAALSGPSAVIAALCALLVCGLGAWIWRRWGTLDRGRATRAVALALG
                     LALVIGSTAWAAVFASGSRAVAAPTSLASEPAASWQPWSPEREAELRSSGTPVLVDFS
                     ARWCLSCQVNERVALYNPSVLKRLQELGTASLKADWTDNDPRVAKALALFGRASVPLY
                     VLYPAGGAQPVLLPEILTPGIVLSALERARSP"
     gene            3984..4439
                     /locus_tag="VFH83_00080"
     CDS             3984..4439
                     /locus_tag="VFH83_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HET6484780.1"
                     /translation="MRITTKGRYGLRAVINLAMARHNRPVSISSIANEEKVSSEFLEQ
                     IFFKLKKAGLIRSVRGPGGGFVLERKPSEITVHDILYAVGEMKGLTPCTLRRRALCDR
                     EEPCSAHEIWVGLQKAMEDYLRGITLKSILDQNGKTFLASLDVGQDFSI"
     gene            complement(4473..5852)
                     /locus_tag="VFH83_00085"
     CDS             complement(4473..5852)
                     /locus_tag="VFH83_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET6484781.1"
                     /translation="MPRYYYREFLDRFDDKGLLSCVCGQEHRLTTKSVLTDPGSLAAS
                     ARLVTERYGKAARVWVLSDENTEAAAGARWKAELRVGKVVSRVLPGTPRPIPSVEFAS
                     SLLAEAKGATPDLLIAVGGGVISDLVKRASLEGGIPNWCIATAPSVDAYSSATSALRV
                     NGFHGTASARTSEMIVCDPEVMANAPKEMFLAGLGDLLAKFHAAIDWGLSRIVTGEHF
                     CPLLAEVALGSARSALAAAKGLAADPTESLRTLTDAVLSSGFAMQAAGGSRCAASAEH
                     TIAHFWETIHAAQVKRLDLHGMLAATASRILLPIYQALYRRLATWQPDPAARLADFDR
                     EPSWEGSLEDGMLPYRAKIQEEMKDYRLTRDMLSNRLARFASQRKAILDLASPLLSEL
                     DAAARQLQEMRYPFSLGELGIPKDLALLPLRSVRLLRNRYTGFDLAYELGLWGFMQEQ
                     MESAVAAVA"
BASE COUNT         1121 a         1966 c         2019 g          991 t
ORIGIN      
        1 ccgcgccgat ggtactgccc tttgagggtg ggagagtagg tcgttgccag gcttttttat
       61 ttgccaaaga gcgtgagtcc gcccatcgat tcttgcagca attctaacta tagggctttc
      121 cgttaagtat taccggtaac cctgtctagt attctggatt gacgtgatga cctaccctct
      181 ggttcaggaa tgtctggagg ggagacgttt cagcttctga tgtcctacct catggcctgc
      241 gtggagggct tccccgatcg gcgcaagggc aggaatatcc agttctcgct tcacgatgtg
      301 acgcgggggg ctttcggagt attcttctgc cagagtccat cctttctggc cttccaagag
      361 ttgatgcaac agcagcaggg gaagaacaac gcacggacgt tgtttggggt tgagcagatt
      421 ccttccgaca accacatccg agcacttctt gaccccgtag atcctgcacg gttgaacgct
      481 gtttaccaga agtgtttcga gctgatgcag cagcacgggc tggtgcatcc catgagggct
      541 gtgggcaact cgctgttgat cgctatggat gccaccgggt actttctctc cgagtccatt
      601 cactgtgact cctgcatggt gagcaaacac cgcgatgggc gcgtgacgta cagccacgcg
      661 gctgtgcttc ctgcggtggt cagcccccag cacagccatg tcatccctct gcaaccggag
      721 tttctccgtc gccaggatgg ccatgagcgg caggactgcg agtttcaagc ggccctgcga
      781 tggataaagg accacggtgc gcagctcagc ccgttgggag tgacgttgct gggagacgat
      841 ctatactcgc gtacgcccct catccgagag gtgcagcagc aagagttgga cttcatcttt
      901 gttgccaagg cggtcatgca caagcatttg caggaggagg tcgaggggct ggagaagctg
      961 ggtggagtgc agcagttgca gcagatcgcc tggaccggcc gccatcaccg ccactaccac
     1021 taccgctggg tccgcgaggt cagtttgacg gcagaaaagg acagccccgt ggtctcatgg
     1081 gtggagttgc agatcctgga cgaggcgggc aagattacct tccgcaacag ctgggtcacc
     1141 agccatccca tcaacgaaca gacggtcgtg gaactagttc gggcagcccg cagtcgatgg
     1201 aagattgaga acgaggattt caacacgctg aagaccaagg gctaccattt cgagcataac
     1261 tttggtcatg gccagcagta cctgtcgcag acgctactat cgctgaatgt gattgccttc
     1321 ctcttccaca ccctcttgga gctcatggac aagcgctgcg ccttgcttcg ctctacgctg
     1381 ccgcggcgtg acacgttctt tcagcatgta gccaccctga cgcagtacct gtgctttgat
     1441 ggttgggcag cgctgatgct gttcatgctc aacggtctac acctccctga ccctgacgga
     1501 tagctcaaca cccatccttc ctcactctcc tctccccgtc ccacctcccg accctgacac
     1561 ctctctgtca cccccggcgc gcctacagcc ttcagcgccc ttcacctcgt ttctctggcc
     1621 ttcggcaccc tagcttccgg gccagctacc ccaacgcccc atcgttctcc tcacagccct
     1681 ggcgtgccaa ctgcgccctg aatgtcaaac tgagaattgc tgggcgggcc gcagagggct
     1741 ggaccgcggg cggccggttg cataccgcgg gcggcgggcc gacagcaggc ggacatcagg
     1801 gggaccgggc acgctccagc gcggagagca cgatcccggg agtgaggatc tccggcagga
     1861 gaaccggctg ggcgccgccg gcgggataca gcacgtagag gggaacgctg gcccggccga
     1921 acagcgcgag cgcctttgcc acccgcggat cgttgtcggt ccagtccgcc ttgagcgacg
     1981 cggtcccaag ctcctggagc cgcttcagga ccgatggatt gtacagggca acgcgctcgt
     2041 tcacctggca gctcaggcac cagcgcgcgc tgaagtctac gagcaccggc gtgccggagg
     2101 atcgcagctc cgcttctcgc tcggggctcc acggctgcca ggacgcggcg ggctccgagg
     2161 ccagcgacgt cggcgcggcc accgcccgtg agccgctggc aaacaccgcg gcccatgcgg
     2221 tgctcccgat cacgagcgcc agcccaaggg ccagagcaac ggcccgggtg gcgcggcccc
     2281 ggtccagcgt tccccaccgt cgccagatcc atgcgcccag accgcacacc aggagcgcgc
     2341 aaagcgctgc gatcaccgcg gagggacccg atagagcggc caggacgaag agcatccaga
     2401 tgaccgcggc catcatcgga aagccgagga tctgcttcag cgtctccatc cagggtccgg
     2461 gcttgggcag ccgggaggag aggcccggcg cggcggagag caccacgtac ggggctgcca
     2521 tgccgatccc gagcgcggtg aaaacaccga aggcgaccgg gaggggctgg cccagcgcgt
     2581 acccaagcgc cgcgcccatg aagggagccg tgcacggcgt ggccactgcc gtggcgaaca
     2641 acccgctcag gaacgaacca gcggtgcgcg gtccggagat cgcccggggc gcgaaagccc
     2701 ggggcgcgga agcccggggc gcgaaagctt ggagctcgaa gagcccgaag agactcgcgc
     2761 cgatcaggaa gaacagcgcc gccgcgatgg caaccacgac gggattctgg aactggaacc
     2821 cccaacccag gctctgtccg ccggcgcgca gggccagaag cacggccgcg attgcccaga
     2881 aggagacaag gacgcccgcg gcaaacagga gcccctgccc tatggcgctc gaatgtccgt
     2941 cgggcgcgtg ccggacgagc cccaggacct tgagcgacac aaccggcagg acgcagggca
     3001 tcaggttgag gatgaccccc ccgatgaagg cgagcaccag cgcaagaagg atcccgaggc
     3061 cggccgccgg cgctgcgctg acgggaaggt ccacatccag cgcccgcgtc gatccaccgt
     3121 ccgagaccac gaggatcccg gtcaggcgct ggccggtccc ggcggctcgc ctgagcgtga
     3181 gcacccgatc cggacccgtc cggcccgtcg tctgcggagc tttgagatcg atctgaccgg
     3241 ccgacgccgg gtagaaggcc acctgcgccg cggcggcctg cagcccgagg atccgaagct
     3301 gaaccgcgct gggagtagag gttgccgtga gcatgcccgc cggggcgctc gaaggaaggg
     3361 cctggcgagc gcggcgaaag gctccgatca aggacgggtc gggggctgga gcgccggtgc
     3421 cgatcgggac cgaagccgtc agctcggcct tccccggcgt acactcgatc cggcaggcca
     3481 gccaggaaac cgtgacagcc agggtcgcct gcttctgcca aagccgagcg ggcggggtga
     3541 tatccaccag caacatgacg tcaccggggt agccgtaagt gacgtcgccc agggtttcga
     3601 tcctctgagg cacgggccac tggatgggtc ccgccgcgaa gccgggcggc aggtgccact
     3661 ctagcgtggt tggcagcccc gaatcaccgg ggtacttcca atacgtgtgc cagtccgggt
     3721 tcgagaccag gtggacgccg acggtgaacg tctggccggc ggcgatcgcg gaagcttcgg
     3781 agagaagctc ggcccggacc gggccgactc ggagaggctc cccgaacgcg gaggtggacc
     3841 cggccgccgc cagaagggca aggcacaccc atgccgtgag gcctcgtccg gcggcaatat
     3901 gagcgagggg cttgcatttt ccgcggcttc ggtggaacat cccttacgaa tacgataata
     3961 caactcaggc ggagcttcaa aggatgagga ttaccacgaa ggggagatac gggcttcggg
     4021 ccgtgatcaa ccttgccatg gcccgtcaca atcgccctgt ctccatcagc tcgatcgcga
     4081 acgaggagaa ggtctcctcc gagtttctgg agcagatctt cttcaagctc aagaaagcgg
     4141 ggctgatccg atccgtccgg ggccctgggg gcggatttgt cctggagcgc aagccgtctg
     4201 agatcaccgt ccacgacatc ctctacgcgg tcggggagat gaaagggctc acaccctgca
     4261 ccctgaggcg gcgagccctc tgcgaccgcg aagagccctg ctccgcgcat gagatctggg
     4321 tcgggctgca gaaggccatg gaagactacc tgcgcggcat caccctcaag agcatcctgg
     4381 atcagaacgg caagacgttc ctggcatccc tggacgtggg ccaggacttt tcgatctgac
     4441 cggcggccaa catcgccgcg gcggcccgcc ggtcaggcta cggccgcgac ggccgactcc
     4501 atctgctcct gcatgaatcc ccagagaccg agctcgtagg ccagatcgaa ccccgtatag
     4561 cggttcctga ggagccgcac gctgcggagc gggagaagcg ccagatcctt gggaattccc
     4621 agctctccga gagagaacgg gtagcgcatc tcctggagct ggcgggccgc cgcgtcgagc
     4681 tccgacagga gaggactggc gagatccagg atcgctttcc tctggctggc gaagcgcgcg
     4741 aggcggttgg aaagcatatc ccgggtgagc cggtagtcct tcatctcctc ctggatcttc
     4801 gcgcgatacg gcagcatccc atcctccagc gagccctccc aggagggttc gcgatcgaaa
     4861 tccgcgaggc gggccgccgg gtccggctgc caggtggcca ggcgacgata gagggcctgg
     4921 tagatgggaa gcaggatgcg gctggcggtt gctgccagca tgccgtggag gtcgaggcgc
     4981 ttgacctgcg ccgcgtggat ggtctcccag aagtgggcga tggtgtgctc cgcggaagcc
     5041 gcgcagcgcg agccgcccgc cgcctgcatc gcgaagcccg agctgagcac cgcatcggtg
     5101 agcgtacgca gggactcggt cgggtccgcg gcgagcccct tggcggcggc cagcgcgctc
     5161 ctcgccgagc cgagagccac ctcggcgagg agcgggcaga agtgctcgcc cgtgacgatg
     5221 cgggacagcc cccaatcgat ggcggcgtgg aacttggcca gcaggtctcc cagcccggcc
     5281 aggaacatct ccttgggcgc gttggccatc acctcgggat cacagacgat catctccgag
     5341 gtccgagccg aggcggtgcc gtggaacccg ttcacccgga gggccgaggt ggccgagctg
     5401 taggcgtcca cggacggcgc cgtggcgatg caccaattcg ggatcccgcc ctctagggat
     5461 gcgcgcttca ccaggtcgct gatgacccct ccgccaacgg cgatcagcag gtccggcgtc
     5521 gcgccctttg cctcggccag gaggctcgat gcgaactcga cggaggggat cgggcggggc
     5581 gtgcccggca ggacccggga cacgaccttt cccacccgca gctcagcctt ccaccgggct
     5641 cctgccgcgg cttccgtgtt ctcatcgctc aggacccaga cccgcgcggc cttgccgtag
     5701 cgctccgtga cgagccgggc ggaggctgcc aggctgcccg ggtccgtcag cacggacttc
     5761 gttgtcagcc gatgctcctg cccgcagaca caggagagga ggcctttgtc gtcgaaccgg
     5821 tccaggaact ctctgtagta gtatcgtggc acgtggggac tatacccacg cgggatggcg
     5881 tcgtccacct cggcggtcag taccggcagg gcttcctgag gctctccttt ttcggatatc
     5941 tttgcgattg gaggagaacc atggtggtgg gacaggggcg agccgacgcc agtctcgatg
     6001 agagggaagt ccagaggatc gtggaagagg gggtgccgcc gaagctgttc gtcggcaaga
     6061 aggtgctggt cctgactccg gactccacgc gcacgtt
//
LOCUS       DASRZS010000006         3347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2697176, whole genome shotgun sequence.
ACCESSION   DASRZS010000006 DASRZS010000000
VERSION     DASRZS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2697176"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFH83_00090"
     CDS             <1..492
                     /locus_tag="VFH83_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014370468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6484782.1"
                     /translation="IINILAGRLGLPKVYYMGSLPWFRPVYVLSDIWQETGWGAIIYL
                     AALSGINPELYEAATMDGATKFQRILHIDIPAILPTVTILLVLRAGSIMSLGFEKAFL
                     MQTPLNLDYSEIISTYVYKVGLQGAQFSFGSAVGLFNSVINLVLLLVVNRVARALGGT
                     SLW"
     gene            506..1384
                     /locus_tag="VFH83_00095"
     CDS             506..1384
                     /locus_tag="VFH83_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6484783.1"
                     /translation="MRRARADVIFDTANVFIVSLFFLLILYPLYFILIASISNPDYVN
                     SGAITFLPRGLTLEGYARTFREASVWRGYRNTILYTALGTTINVSLTITAGYALSRRE
                     LVGRSLIMRLIVFTMLFSGGLIPTYLIVKGLGMTNTIWAMVIPNAVSAWNLIIARTYL
                     QASIPPELLEAALVDGCSYGRFFWEVVVPLSGAIIAVLALFYSVAHWNSFFNGLIYLQ
                     DYARYPLQLILRDILLGYQASGAADDAKTLQEMQRIADLMKYALILVASLPVMVLYPF
                     LQRYFVKGVMIGAIKG"
     gene            1512..1970
                     /locus_tag="VFH83_00100"
     CDS             1512..1970
                     /locus_tag="VFH83_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6484784.1"
                     /translation="MKAGRTEHADEAQLKEVVELLRDVLHGLLMTSVPAWLDLQLTLP
                     QLRAVFVVAHHEGCSLREVAGHLGVGEPTASHLIDRLVTQGLIERGDDPEDRRRAVLR
                     LSAGGRKLITRLLGWENILATWLREIPRADLASLSRGLSAVRDNVRRKLP"
     gene            1967..2776
                     /locus_tag="VFH83_00105"
     CDS             1967..2776
                     /locus_tag="VFH83_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET6484785.1"
                     /translation="MIGGIDPSFLWLPLVGAAIGLLVTVFGGGGGFFYVPILTLLFHV
                     PTQLAAATSLAATIPTVIVGSIEHYRKGNIDVRIGAIFGVAGLLGAFLGAWGSSLVSS
                     DLLRKLFGAYAIALTVPMVLTSRNRLKKQAREAVTPLPLTAAHIALSVSFGLLSGIMA
                     GLFGTSGTASIVAGLYILGLPVTVVVGTSVAVVLFNAVSGLAGHLVVGRVDVPLLVLL
                     GIGSVVGGFFGPRLLARINVEALEKIYGVLFILLVIVFGLLMLLGSPHAAR"
     gene            2895..>3347
                     /locus_tag="VFH83_00110"
     CDS             2895..>3347
                     /locus_tag="VFH83_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HET6484786.1"
                     /translation="MSGNAARRRFPTPYVVLGVIVAAAAAAWFAVPRPQKEEAAPRVF
                     PVETVTPQIGSLERSLTVDSYVQSDSVVTVLPKVAGTIVSFSADVGTHLEAGQVIATV
                     DPEPYRLTVDQAQAAFGAAESVWERVRKLHDSGAASQQSFDQAQAQYDD"
BASE COUNT          511 a         1104 c         1065 g          667 t
ORIGIN      
        1 atcatcaaca tcctggcggg aaggctgggc ctcccgaagg tctactacat ggggagcctg
       61 ccgtggttcc gcccggtgta tgtcctctcc gacatctggc aggagaccgg gtggggagcc
      121 atcatctacc tggccgccct ctcgggaatc aaccccgagc tgtatgaggc ggccaccatg
      181 gacggcgcca ccaagttcca gcggatcctc cacatcgaca tcccggccat cctgcccacc
      241 gtgaccatcc tgctggtgct gcgcgccggc tccatcatgt ccctggggtt cgagaaggcc
      301 ttcctgatgc agacgcccct gaacctcgac tactctgaga tcatctccac gtacgtctac
      361 aaggtcgggc tgcagggagc ccagttcagc tttggctccg cggtcgggct gttcaactcg
      421 gtgatcaacc tcgtgctgct cctggtggtg aaccgggttg cgcgcgccct tggcgggacg
      481 agcctctggt gaggggagac cggcaatgag gcgagcgcgc gcagacgtga tcttcgacac
      541 cgccaacgtc tttatcgtct ctctgttctt cctcctgatc ctctacccgc tgtacttcat
      601 cctgattgcc tccatcagca acccggacta cgtcaattcc ggcgccatca ccttcctccc
      661 ccgtgggctg acgctggagg gatacgcccg gaccttccgg gaagccagcg tgtggcgagg
      721 gtaccgcaac accatccttt acacggcact gggtacgacc atcaacgtgt ccctgaccat
      781 taccgccggc tacgcgttgt cccggcggga gctggtgggg cggtccctga tcatgcgcct
      841 gatcgtattc acgatgctct tcagcggcgg gctgatcccg acctacctga tcgtgaaggg
      901 tctcgggatg acgaacacca tctgggccat ggtcatcccg aacgcggtat cggcgtggaa
      961 cctgatcatc gcccgcacgt acctgcaggc gagcatcccc ccggagctcc tggaggcggc
     1021 cctcgtggac gggtgctcct acggccggtt cttctgggaa gtcgtcgtcc cgctgtcggg
     1081 ggccatcatt gccgtgctcg cgctattcta ctccgtggcc cactggaact cgttcttcaa
     1141 cggcttgatc tacctgcagg attacgcccg ctacccgctt cagctcatcc ttcgtgacat
     1201 cctgctcggc taccaggcca gcggcgcggc ggatgacgcc aagacgctgc aggagatgca
     1261 gcgcattgcg gacctcatga agtacgcgct catcctggtg gcctccctcc cggtgatggt
     1321 gctttacccc ttcctccagc gctacttcgt gaagggcgtc atgatcggcg ccatcaaggg
     1381 ctgagacgcg gccaacccat cggcgcggac cgcgcgccag cggtcccgtc ccggaaatcc
     1441 ttgacgtacg tcatttaatt cgctaatatc gaattgttcg aattaatcga tcttgttggg
     1501 agaaggcgga gatgaaggca ggcaggacgg agcacgcgga cgaggcgcag ttgaaagagg
     1561 tggtggagct gctgagggac gttctccatg gcctcctcat gacctccgtc ccggcgtggc
     1621 tcgacctgca gctcacgctc ccccagcttc gggccgtctt tgtggtggcc caccacgaag
     1681 gctgctcgct gcgggaggtt gccgggcacc tgggggtcgg ggagcccacg gccagccacc
     1741 tcatcgaccg gcttgtgacc caggggctga tagagcgggg agacgacccc gaggaccggc
     1801 ggcgcgcagt cctgcggctc tcggccgggg gcaggaagct gatcaccagg ctcctgggct
     1861 gggagaacat ccttgccacg tggctgcgag agatcccccg ggctgacctg gcgtccctca
     1921 gccgcggcct gagcgccgtc cgggacaacg tgcgacggaa gttgccatga tcggcggcat
     1981 tgatccttcc tttctctggc tgcctctcgt gggggctgcc atcggactgc tcgtgacggt
     2041 attcggaggc ggaggggggt tcttctacgt gcccatcctc acgctgctct ttcacgttcc
     2101 cacgcaactc gcggccgcca cctccctggc ggccaccatc cccaccgtga tcgtgggctc
     2161 catcgagcac tatcgcaagg gcaatatcga cgtccgcatc ggggccatct tcggggttgc
     2221 gggcctgctg ggggcgttcc tcggcgcgtg gggctccagc ctcgtctctt cggatctgct
     2281 tcgcaagctg tttggggcgt acgccatcgc gctgacggtc cccatggtgc tcaccagccg
     2341 aaaccggctc aagaaacagg cccgcgaggc agtgaccccg ctccccctca cggcggcgca
     2401 cattgccctt tccgtctcgt tcggcctgct gtccgggatc atggcgggtc tctttggaac
     2461 cagcggcacc gcctccattg tggccgggct gtacatcctc ggcctgcccg tgacggtggt
     2521 ggtgggcacc tccgtggccg tggtgctgtt caacgccgtg tccggtcttg cggggcacct
     2581 cgtagtggga cgggtggacg tgcccctgct cgtgctcctg ggcatcggat ccgtcgtggg
     2641 cgggttcttc ggaccgcgcc tcctggcccg gatcaacgtg gaagcgctgg agaagatcta
     2701 cggagtcctc ttcatcctcc tggtgatcgt gttcggcctc ctcatgctcc tcggctcgcc
     2761 gcacgccgcc cgctgagcgg ccggccgcgg cggatcggcg cttgattgtt gtctaatagt
     2821 tcgatataat cgaattaatt gttttttacg atctaaatga tgcacccgcg ggtgccatcg
     2881 ggagggtgtg aatcatgagc gggaatgcag ctcgccggag gtttcccacg ccgtacgtgg
     2941 tgctgggtgt gatcgtggcc gccgcggcag ccgcctggtt tgccgtcccg cgcccgcaga
     3001 aggaagaggc ggcgccgcgc gtgtttcccg tggagaccgt gacgccgcag atcggctccc
     3061 tcgagaggtc gctcaccgtc gattcctacg tgcagtccga ctccgtggtc acggtgctcc
     3121 cgaaggtggc cgggaccatc gtgtccttct ctgcggacgt ggggacgcac ctggaagcgg
     3181 ggcaggtgat cgctaccgtt gacccggagc cctaccgact caccgtggac caggcgcagg
     3241 cagccttcgg ggcggcggag tcggtgtggg agcgggtccg gaagctgcat gactcgggag
     3301 ccgccagcca gcagagcttc gaccaggcgc aggcgcagta cgacgat
//
LOCUS       DASRZS010000007         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_773828, whole genome shotgun sequence.
ACCESSION   DASRZS010000007 DASRZS010000000
VERSION     DASRZS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773828"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1146)
                     /locus_tag="VFH83_00115"
     CDS             complement(7..1146)
                     /locus_tag="VFH83_00115"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HET6484787.1"
                     /translation="MFGEPSNPLYVVPKGVETRWASPENPTGDRGSAARSNGGRKGSA
                     FFPLKAGETRVLASAAGASGVVRRIWVTFQPITSLFLRGVRLDFFFDGDRTPTFSVPF
                     GDFFGVGLGRMTDFVTALFAAPEGKSFNCLVPMPFRRSMRIAITNETLEDIRLFFYDV
                     DYTLGDEIGPDCLYFHAHYRRENPTREKEDYAFLPRIQGRGRFLGLNAGVIADRARFY
                     QSWWGEGEVKCYIDGDGELPTLCGTGSEDYVGTGWGLKRFIMPYQGCTLSDGFNMWYC
                     FYRYHVPDPIFFSREIRVTIQQIGCWGPDTKPLLRDSGRPVYYARPGLQQVDFSPSGD
                     PEKYGLFERHDDWSSCAYFYLDRTESGLPSLPALADRIANLIDVI"
     gene            complement(1194..1322)
                     /locus_tag="VFH83_00120"
     CDS             complement(1194..1322)
                     /locus_tag="VFH83_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484788.1"
                     /translation="MDCRGIDIAVDGGGLSIMLGAPQGFESRPIDVDRFYQAHWRR"
     gene            complement(1396..>2375)
                     /locus_tag="VFH83_00125"
     CDS             complement(1396..>2375)
                     /locus_tag="VFH83_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6484789.1"
                     /translation="TLGLVGESGCGKTTTGRCLLRAVEPTAGSIVAGLRDGRRLSVTT
                     AGAEDLRTFRKEAQMIFQDPSSSLSPRMSVFDIVAEPLVVNQVMGGRALQDRVAELLT
                     MVGLEPEHMRRYPHAFSGGQRQRIGIARALALNPRIIIADEPVSSLDVSIQAQIIALL
                     KDFQERLGLSLLFITHDLRVVHHVCERVAVMYAGQIVEMASRTQLFETPSHPYTAALI
                     SAVPKILVDRSARPQRVVLRGEPVDLANIPAGCRFADRCSFVQEVCRQRMPELRTLAD
                     GRVARCHFAGGLPLKGIPEESLFEFEDIDLLIEQTTGERRQGGSTGCGP"
BASE COUNT          429 a          782 c          791 g          373 t
ORIGIN      
        1 cccctttcag atcacgtcga tgaggttcgc gatgcggtcg gcaagcgccg gcagcgacgg
       61 cagcccgctc tccgtccggt ccaggtagaa gtacgcgcag ctcgaccagt cgtcgtgccg
      121 ctcgaaaagc ccgtacttct ccgggtctcc gctcggagag aaatccacct gctggaggcc
      181 gggccgcgcg tagtagacgg gccgtccgga gtcgcgcagg aggggcttgg tatccggtcc
      241 ccagcacccg atctgctgga tggtgacccg gatctcgcgg gaaaagaaga tggggtccgg
      301 cacgtggtag cggtagaagc agtaccacat gttgaagccg tcggagaggg tgcatccctg
      361 gtaaggcatg atgaatcgct tcagacccca gccggtcccc acataatcct ccgagccggt
      421 gccgcatagc gtgggcagct ctccatcgcc gtcgatgtag cacttcacct cgccctcgcc
      481 ccaccagctc tggtagaacc gggcacggtc ggcgatcacg cccgcgttca gccccaggaa
      541 ccggcccctg ccctggatcc ggggaaggaa cgcgtagtcc tccttctccc gggtcgggtt
      601 ctcacgccgg tagtgcgcgt ggaagtacag gcaatccgga ccgatctcgt ctcccagggt
      661 gtagtccacg tcgtagaaga agaggcggat gtcctccaac gtctcgttgg tgatcgcgat
      721 ccgcatggag cgtcgaaacg gcatggggac caaacaattg aagctcttgc cctccggagc
      781 ggcaaagagc gccgtgacga aatccgtcat gcggcccaga cccacgccaa agaagtcccc
      841 gaagggtacg ctgaaggtcg gggtccggtc cccatcgaag aagaagtcca gccgaacacc
      901 gcgcaggaag agagaggtga tgggctggaa ggtcacccag atccgccgga ccacgccgct
      961 cgccccggcg gcggatgcca gcacccgggt ctcaccggcc ttcaggggaa aaaaggcgga
     1021 gcccttgcgc ccgccgttgg agcgtgccgc ggatccccgg tccccggtgg gattctccgg
     1081 gcttgcccat cgggtctcga cgcccttggg cacgacgtag agcgggttgg atggttcacc
     1141 gaacatgatc gcctccctga gctttgctga acaccgcgag cgaactggcg ccattacctt
     1201 cgccagtgcg cctgatagaa ccgatccacg tcgatgggcc tcgactcgaa gccctggggc
     1261 gctcccagca tgatggagag cccgccacca tcgacggcga tgtcgatgcc tctacagtcc
     1321 atgatctcga cggcgagccc ggcctgggcg gcacccaggg ggtatgcgca tccacaacgg
     1381 ccaaggaggt gccgtctacg gtccgcatcc ggtgctcccg ccctggcggc gctctccggt
     1441 ggtctgttca atgaggagat cgatgtcctc gaactcgaag agcgattcct cgggaatccc
     1501 cttcagggga aggcctccgg caaaatgaca ccgggccacg cgcccgtcgg ccagggtgcg
     1561 cagctccggc atcctctgtc ggcagacctc ctggacaaag gagcatcggt ccgcgaaccg
     1621 acaccccgcg gggatgttcg ccaggtccac gggctctccg cgaagcacga ccctctgggg
     1681 ccgggcgctg cgatccacca ggatcttggg cacggcggag atgagcgccg cggtataggg
     1741 gtggctgggg gtctcgaaga gctgagtccg gcttgccatc tccacgatct ggccggcgta
     1801 catgaccgcc acccgttcgc agacgtggtg caccacgcgg aggtcatggg tgatgaagag
     1861 caggctcaag cccagccgct cctggaagtc cttgagaagc gcgatgatct gggcctggat
     1921 cgaaacatcg agggaggaca ccggctcgtc ggcaatgatg atccgggggt tgagcgccag
     1981 ggcgcgcgca atcccgatgc gctgccgctg gccgccgctg aaggcgtgcg gatagcggcg
     2041 catgtgctcg ggctccagtc ccaccatcgt gaggagctcg gcaacccggt cctgaagggc
     2101 gcggcctccc atgacctggt tcaccacgag cggctcggcc acgatgtcga acacggacat
     2161 ccgggggctc agcgaggagg acggatcctg gaagatcatc tgcgcttcct tgcggaaggt
     2221 ccgaagatcc tcggcgccgg ccgtcgtgac ggaaagccgc cggccgtccc ggagcccggc
     2281 cacgatggac cccgcggtcg gctcgacggc gcggagcagg caccggccgg tcgtggtctt
     2341 tccgcagccg gactcgccga cgaggccgag cgtct
//
LOCUS       DASRZS010000008        10392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4237154, whole genome shotgun sequence.
ACCESSION   DASRZS010000008 DASRZS010000000
VERSION     DASRZS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10392
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4237154"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VFH83_00130"
     CDS             complement(<1..1012)
                     /locus_tag="VFH83_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6484790.1"
                     /translation="MKRIIVALTIAVLACAALGAQAKDPLGKYSPTITITTVKSQGDS
                     VKFDEGEDYNNNIWTRGYLSYLGIKGQVLWAVPTSQFQQKMNISIAANDLPDIIPADL
                     RQFKQLVDMGVATDMTQIFAGYVSPLTKQMFDLDQGVALGQATIKGKLMGIPQLAGNG
                     DTPAVIWIRADWLKKLGLQPPKTMADLFAIMDAFTNKDPNGDGQKNTYGLALEKNLLG
                     GGLHDLDGFFNGYHAYANDADWVKDDSGKLVYGTIQPQVRQGLAKLAELYKNGEIDPE
                     FAVKDASKVTEFTTSGRLGVEFGQHWNSFWPLQDSKNKDPNADWKPYPLVSIDSKPAM
                     PIT"
     gene            1205..2926
                     /locus_tag="VFH83_00135"
     CDS             1205..2926
                     /locus_tag="VFH83_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET6484791.1"
                     /translation="MAFPAPRGAPLFTKVVLAFCAVLVPLYLLGSLIYLAGAQALRNR
                     LLQAEVSQLQTAAHRLEQEYEHVATMQRNYVENFGLRRFAVASGIMTTYDRMQSILAV
                     QDDLTLLCAASPIVADATVDFPLLGRRVSRSQADSYPPEAGRVWNGGPQFLPRTLELR
                     TVYPSNAAVAGVQFVLSITLDASQVAAIIASSTLSPSGQAFLTDASGAVLFGKPREPV
                     GAQSIQDAVSRAVGTRTGQVSIRAGRTAYLVSVVPVEGMSVWLTSVSPEWETLGPLSG
                     YRRWFWALTALTAGLGGLFAFWISSAVRRPLAKLMGGFREVEAGDLGVQLSWEHRDEF
                     GYLFARFNAMAASLDATLRRCIEQESLARHAELLQLQSQINPHFLFNSIYHIYRMAKD
                     EDVPRIERYASHLGSYFEFITRGAGEDTSLAEEARHAGNYSEVQRIRFEDRISVDLGE
                     VPERLRPLRVPRLIVQPLLENAYNHGLKDVVANGVVVVRYRDEGELAAVTVEDNGSGM
                     TREELDRLSALLQIDSIPTGTAATGILNIHRRLALRFGPPGGVRLECSPLGGLRVSLL
                     IPLREAA"
     gene            2928..4181
                     /locus_tag="VFH83_00140"
     CDS             2928..4181
                     /locus_tag="VFH83_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6484792.1"
                     /translation="MHRILVVDDETSIATSLAASLSEELAAGQFEVSAAFSAADALRR
                     MEQRRTDIVISDIQMPRMDGLELGRAVQARWPDCRFIFLTGHGEFRYAQEALRAGCVD
                     YILKTEGDERVLAVVRETARRLDAERLDREQRVQEQRALEDLRRASARRYLADFARGR
                     APARQEASEQAPEALPRILSHFGAEALSQGRLFVAVLVTTSPIEAVLSAAAAAFLPAA
                     VVLDFPLDELSGVFLAAAPPVSPTDPTLVRGILESVQDTIRSGAQPFSFALDPEPVDL
                     SALPARVARLEACLAGRSEAAGAAIVLGADPAGPLSAGLDLVAQVNAYVDAHLAEDVS
                     LTRLAEAVSLNPSYLSRLYLQRAGIHLSERIASVRLEQAKALLAEGRLKVSEIAAAVG
                     FWTPSHFIRFFKQRTGVTPQAFRST"
     gene            complement(4160..4702)
                     /locus_tag="VFH83_00145"
     CDS             complement(4160..4702)
                     /locus_tag="VFH83_00145"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6484793.1"
                     /translation="MPKSTSRADTAMLIVFRGLPGTGKSHLARAVIARRPSLLVLSRD
                     TLRRTMIAHPTFDEEEKALVDELILSMVGFLMDRGRDVVIDGMALSSAAQVEEFAVAA
                     ESRGAGARIIECVCSERTALARITADLGTHPAGDRGAALYHSVKARFQPLARPSLVID
                     TEADLDANVERVLGYVDRNA"
     gene            complement(4728..5591)
                     /locus_tag="VFH83_00150"
     CDS             complement(4728..5591)
                     /locus_tag="VFH83_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HET6484794.1"
                     /translation="MLSLKQAIDALNAKHPQSAIGAFNFNDMTDMHGVMNAAMKKRMP
                     LILMASSSAVKYMGIHFVREMYRAATMTSDVPLYLMLDHSESIETCAQCIDNGFSLVM
                     YDGSALSFDENVRNTREVIRIAHARGVLVEAEIGRLPGREEHLNVSEMDALATDPKEA
                     QSFWDSTGVDLLAISFGTAHGFYTSEPRLNYEVIQRTRELTDAPLVMHGGTGVPDADL
                     RQAIGCGVKKVNVGTEIKHAYTSALREALAKAPDEIDPRKILIKAKEAVQAAVEGRLA
                     VLGTPRLEELV"
     gene            complement(5677..7032)
                     /locus_tag="VFH83_00155"
     CDS             complement(5677..7032)
                     /locus_tag="VFH83_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HET6484795.1"
                     /translation="MTILSSTLSPKRIVTLRSRTKDGALRELATTLSLGGPRERDGLL
                     RVIRERERVLGSWIAPGVAIPHVKLPNWEGIQVAVGRSRRGIRWDSSDGNPVNLLILI
                     VSGVNEPDRHVLLLAEIARTLHDRALMQLVLTARTPLESYRLLTMRGHVSQDQGPSSP
                     KLRLSRLLFAHALSVAEEVKAKAVVLHTDAVGSLHFIEDLPAQGQLILVSSSKQEPTP
                     QDGPGIHRLQVPFHTLDRHGQVDLALLFAISRGLLARGDTVVSLSGVPSSGTLDTLLV
                     IDVGREFPTVFVRPAVAPLGDVEPQVLEKVLQIAEGIAREGREGRPVGTAFVVGDYDH
                     VLQYCRQMVINPFHGYGPEEKSILDPSLEETVKEFSTIDGAFVIRGDGVIMSAGTFLR
                     PERSAPSLPSGLGARHAAAAALSQITASLAVVVSQSTGRVSLFKGGGLVMSLGKPGAP
                     P"
     gene            7161..7484
                     /locus_tag="VFH83_00160"
     CDS             7161..7484
                     /locus_tag="VFH83_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HET6484796.1"
                     /translation="MAIEREPLDSFQSDTGVQQKPRLEEPRLYRVILHNDNYTTMDFV
                     VKVIVEVFHKPAAEATKIMLDVHRAGRGIVGVYTLDIATTKVSQVHAMARQNEFPLRA
                     SCEEA"
     gene            7485..9755
                     /gene="clpA"
                     /locus_tag="VFH83_00165"
     CDS             7485..9755
                     /gene="clpA"
                     /locus_tag="VFH83_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HET6484797.1"
                     /translation="MEISTELNHVIMAAYREAESRSHEYMTPEHVLYASLFFDRGREV
                     ITGCGGNVDNLLKDLEEFFRNRVPVVEGSKPIQSAGFQSMMENAILHTASAEKQAVEV
                     DDMLASLLEEKESFAVHLLARQGVDRLSLLSFISHGVTAYPVTEEPADGDAAEDREEE
                     GTGQEGSASATPKAGRALQAFTTDLTEKARSGGIDPLIGREEILQRTIQVLSRRLKNN
                     PIHVGEPGVGKTAITEGLAQRIVDGKVPKALKDFRIYALDMGALLAGTKFRGDFEERL
                     KRVIKELERQQRAILFIDEIHTVVGAGSVSGGSLDASNILKPVLSSGKVRCIGSTTYE
                     DYRKYFDKDRALSRRFQKVEVPEPTAEESRQILLGLRSKYEEHHGVRYTDEAIAAAAE
                     LSARFITDRHLPDKAIDVLDESGAWVALYGPEVEGPRPVEVGVIEQVVARIARIPQAS
                     VSSDERRKLRSLEADLRQRIFGQDHAIDVVVRAIRKSRAGFGEREKPVASLLFVGPTG
                     VGKTELSRQIAQSLGIGLLRFDMSEYQERHTVSRLIGAPPGYVGYDDGGLLTDAIRKQ
                     PHAVLLLDEIEKAHQDIYNMLLQIMDYATLTDNNGKKADFRNIILIMTSNAGAREIGK
                     ALVGFGERSVKEEAVLDAVERIFAPEFRNRLDAVVRFNALGHEQVLAVVDKQLREFQR
                     ELEAKGVSLEVSDACREWLARKGYSEEFGAREIARLISSSIKDFFVDEILFGRLTGGG
                     KTRADLDGEKVVITVL"
     gene            9752..10273
                     /locus_tag="VFH83_00170"
     CDS             9752..10273
                     /locus_tag="VFH83_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET6484798.1"
                     /translation="MNVVAAFLTQTTIDVPVTVFRLVLSFVMGAAIGLEREWHRQSAG
                     LRTHILISLGATMLTLLSIYIPQEFTRYQGIDPGRIAAQVISGIGFLGGGAIFRLGVN
                     VRGLTTAASIWVVAGIGMLVGAGMYFASLAGTGLVLLALIVLVPEGIRWNGLYEKVGR
                     VSGITHISVDQKV"
BASE COUNT         1642 a         3433 c         3539 g         1778 t
ORIGIN      
        1 aggtgatggg catcgcgggc ttggaatcga tggagaccag cggatacggc ttccagtccg
       61 cgttggggtc cttgttcttg ctgtcctgca ggggccagaa cgagttccag tgctggccga
      121 actccacgcc caggcggccg ctggtggtga actccgtcac cttgctcgcg tccttgaccg
      181 cgaactcggg gtcgatctcg ccgtttttgt aaagctccgc cagcttggcc agaccctggc
      241 ggacctgggg ctggatggtc ccgtacacga gcttgccgga gtcgtccttc acccagtcgg
      301 cgtcgttggc gtaggcatgg taaccgttga agaagccgtc caggtcgtgc agaccgccgc
      361 ccaggaggtt cttctccagc gccaggccgt aggtgttctt ctggccgtca ccgttcgggt
      421 ccttgttggt gaacgcgtcc atgatggcaa agaggtccgc catggtcttg ggcggctgca
      481 ggcccagctt cttgagccag tcggcgcgga tccagatcac ggccggagtg tccccgttgc
      541 cggcgagctg cgggatgccc atcagcttgc ccttgatcgt cgcctgcccc agggccacgc
      601 cctggtccag gtcgaacatc tgcttggtga gcggggaaac gtagccggcg aagatctgcg
      661 tcatgtccgt ggcaacgccc atgtccacca gctgcttgaa ctggcgcagg tccgccggga
      721 tgatgtccgg caggtcgttc gccgcgatgg agatgttcat cttctgctgg aactgggagg
      781 ttgggacggc ccacagcacc tggcccttga tcccgaggta gctcaggtat ccgcgggtcc
      841 agatgttgtt gttgtagtcc tcaccctcgt cgaacttcac cgagtcaccc tggctcttca
      901 ccgtggtgat ggtgatggtc gggctgtact tgcccaacgg gtctttggcc tgggctccga
      961 gcgccgcgca cgctagtacg gcgatcgtga gcgccacgat gatccgcttc atagaacctc
     1021 cttggcgtga atcggatgga cgtgcctcgc cgcgccccgc aagcgcacgg attcccgatg
     1081 cagcgggccg cggcggctcc cgacaggata ggaccccccg gaacccccgg tcaatcgcga
     1141 agtgttgaca ttggatcgcg ttctctttac cccgggttcc gcgttcggat acactccgac
     1201 cgtgatggct ttccccgcgc cccggggcgc cccgctgttc accaaggtcg tgctcgcctt
     1261 ctgcgcggtc ctggtccccc tctacctcct ggggagcctg atctacctgg cgggcgccca
     1321 ggcgctcagg aaccggctcc tccaggccga ggtctcccag ctccagaccg cggcgcaccg
     1381 gttggagcag gagtacgagc acgtggccac gatgcagcgg aactatgtcg agaacttcgg
     1441 attacggagg ttcgcggtcg cctcggggat catgaccacc tacgatcgga tgcagtccat
     1501 cctggccgtc caggacgacc tcacgctcct gtgcgccgca agccccatcg ttgccgacgc
     1561 cacggtggac ttcccgctcc tgggtcgccg cgtctcccgg tcccaggccg acagctaccc
     1621 ccctgaggcg gggcgggtct ggaacggcgg tccccagttc ctgccccgga cgctcgagct
     1681 gcgcacggtc tatccctcca acgcggccgt ggcgggggtg cagttcgtgc tctccatcac
     1741 cctggatgcg tcccaggtcg ccgcgatcat cgcctcctct acgctctcgc cgtcggggca
     1801 ggcgttcctc acggacgctt cgggcgccgt cctctttggc aagccgcgcg agccggtggg
     1861 cgcccaatcc atccaggatg ccgtgtcccg cgccgtgggg accaggacgg gtcaggtttc
     1921 catcagggcc gggaggacgg cgtacctggt ttccgtggtg ccggtggagg gcatgtccgt
     1981 ctggctgacc agcgtctcac cggagtggga gaccctgggg ccgctctccg ggtaccgacg
     2041 ctggttctgg gcgctgactg ccctcacggc agggctgggc ggcctgtttg ccttctggat
     2101 ctcctccgcg gtccgaagac cgctggccaa gctgatgggt ggctttcggg aagtggaggc
     2161 gggggacctc ggcgtccagc tctcctggga gcaccgggac gagttcggtt acctcttcgc
     2221 ccggttcaac gccatggcgg cttccctgga cgctacgctc cgccggtgca tagagcagga
     2281 gtccctggcc cggcacgcgg agctcctgca gctccagtcc cagatcaacc cccacttcct
     2341 cttcaacagc atctaccaca tctaccgcat ggcgaaggac gaggatgtcc cgcggatcga
     2401 gcgctatgcc agccacctgg gctcctactt cgagttcatc acgcgcggag cgggggagga
     2461 cacctctctg gccgaggagg cgcggcacgc cggcaactac tccgaggtgc aacgaatccg
     2521 gtttgaggac cggatcagcg tggatcttgg cgaggtcccc gagcgcctgc ggccgttgcg
     2581 cgtcccgcgg ctcatcgtac agccgctgct ggagaacgcg tacaaccacg ggctgaagga
     2641 cgtggttgcc aacggcgtgg tggtggtgcg ctaccgggac gagggcgagc ttgccgcggt
     2701 aacggtcgag gacaacggga gcggcatgac ccgggaggag ctggaccggc tctccgcgct
     2761 gcttcagatc gattccatcc ccacgggcac ggccgccacc ggcatcctga acatccaccg
     2821 caggttggcg ctgcggttcg gtcctccggg cggggtccgc ctggagtgct cccccctggg
     2881 cgggctgagg gtatccctgc tcatcccgct ccgcgaggct gcctgaggtg caccggatcc
     2941 tggtggtgga tgacgagacc tccatcgcca cgagcctggc cgcgagcctc tcggaggagc
     3001 tggctgcggg ccagttcgag gtgagcgcgg cgttctccgc ggcggacgcg cttcgccgga
     3061 tggagcaacg gcgcacggac atcgtgatct ccgacatcca gatgcctcga atggatgggt
     3121 tggagctggg ccgggcggtg caggcgcgat ggccggattg tcgattcatc tttctcaccg
     3181 gtcacgggga gttccgctac gcccaggaag cgctgcgcgc cggctgcgtc gattacatcc
     3241 tgaagacgga gggggacgag cgggtgctgg ccgtggtgcg ggagaccgcg cgccgcctgg
     3301 acgccgagcg cctggatcgc gagcagcgcg tccaggagca gcgggccctg gaagacctgc
     3361 ggcgcgccag cgcgcggcgt tacctggcgg atttcgcgcg cggccgggcc cccgcgcgcc
     3421 aggaggcgtc ggagcaggca ccggaggccc ttcctcgaat cctgtcgcac ttcggggccg
     3481 aggcgctgag ccagggccgc ctcttcgtgg cggtgcttgt cacgacgagc ccgatcgagg
     3541 ccgtcctctc ggccgctgcc gcggcatttc tgccggctgc cgtcgttctg gacttcccgc
     3601 tggatgagct gtccggtgtc tttctggcgg ccgcgccacc cgtctcccct acggacccaa
     3661 cgctcgtccg aggaatcctg gagtccgtcc aggatacgat ccgctcggga gcgcagccct
     3721 tctccttcgc gctggatccg gaacccgtgg atctctcagc ccttcccgcc cgcgttgcgc
     3781 gtctggaggc ctgcctggcc ggtcgatcgg aggcggcggg tgcggcgatc gtgctcggcg
     3841 cggatccagc cggcccgctg tccgcgggtc tggacctggt cgcccaggtg aacgcgtacg
     3901 tcgacgccca cctggccgag gacgtctccc tgactcggct ggcggaggcg gtctcgctca
     3961 acccatccta tctctcgcga ttgtaccttc agcgggccgg cattcacctc tcggagcgca
     4021 tcgcgagcgt gagactggag caggcaaagg cgcttctcgc ggaggggcga ctcaaggtat
     4081 cggagatagc ggcggcggtg gggttctgga ccccatcaca cttcatccgg ttcttcaagc
     4141 agaggaccgg ggttacgcct caggcgttcc ggtccacata gcccaacacc cgttccacgt
     4201 tggcatccag gtccgcctcg gtgtcgatca ccagggacgg gcgcgccagc ggctggaatc
     4261 gcgccttcac ggaatggtag agggcagcgc ctcgatcccc ggccggatgc gtcccgaggt
     4321 ccgcggtgat gcgggccaac gcggtcctct ccgagcacac gcattcgatg atgcgagcgc
     4381 cggccccccg ggactccgcg gcgacggcga actcctccac ctgggccgcg gaggacagcg
     4441 ccatgccgtc gatcacgacg tcccggcccc ggtccatgag gaagcccacc atagagagga
     4501 tcagctcatc gaccagcgct ttctcctcct catcgaaggt gggatgcgcg atcatcgtcc
     4561 tgcggagggt gtcccggctc aggacgagca aggacgggcg gcgcgcgatc acggcgcgcg
     4621 ccaggtggga ctttccggtg cccggaaggc cgcggaacac gatcagcatg gcggtgtccg
     4681 ctcgcgacgt tgacttcggc atccgggcct gcgcgggcgc ggcgccgtca gacgagctcc
     4741 tccagcctcg gagtgcccag aacggcaagt cgtccctcca cggcggcctg gacggcctct
     4801 tttgccttga tgaggatctt gcggggatcg atctcgtcgg gagcctttgc cagcgcctcc
     4861 cgcagcgcgg aggtataggc gtgcttgatc tcggtcccga cgttcacctt cttgaccccg
     4921 cagccaattg cctggcgaag gtccgcgtcc ggcacgccgg tgccgccgtg catgacgagc
     4981 ggcgcgtcgg tgagctcccg cgtccgctgg atcacctcgt agttgagcct gggctcggac
     5041 gtgtagaacc cgtgggccgt gccgaaggag atggccagga gatccacgcc cgtgctatcc
     5101 cagaagctct gggcctcctt ggggtccgtc gccaaggcgt ccatctccga tacgttgagg
     5161 tgctcctccc ggccgggcag ccgcccgatc tccgcctcca ccagcacccc gcgggcgtgg
     5221 gcgatgcgga tcacctcccg ggtgttgcgc acgttctcgt cgaaggaaag cgccgagcca
     5281 tcgtacatca cgagggagaa cccgttgtcg atgcactggg cgcaggtctc gatgctctcg
     5341 ctgtggtcca gcatcaggta cagggggaca tcggaggtca tggtggccgc gcggtacatc
     5401 tcccgaacga agtggatgcc catgtacttc acggcggagg agctggccat gaggatcagc
     5461 ggcatgcgct tcttcatggc agcgttcatg accccgtgca tgtcggtcat gtcgttgaag
     5521 ttgaaggccc cgatggcgct ctggggatgc ttggcattga gcgcatcgat ggcctgcttc
     5581 aggctcagca tctgggatgt ctccttgttc gccctgtact atatcgcgcc gggtgggctg
     5641 tcaagggacg atgggttcga aaagggcccg cagtgactac ggaggcgcgc ccggcttccc
     5701 caggctcatg accagaccgc cacccttgaa gagcgagacc cgccccgtgg actgggagac
     5761 caccaccgcc aggctcgccg tgatctggga gagggccgcc gcggcggcgt ggcgggcgcc
     5821 aaggccggac ggcaggctgg gcgccgaacg ctccgggcgg aggaaggtac cggccgacat
     5881 gatcactccg tccccccgga tcacgaaggc tccgtcgatg gtggagaact ccttcaccgt
     5941 ctcctccagg ctagggtcca ggatgctctt ctcctcgggg ccgtatccat ggaagggatt
     6001 gatcaccatc tgccggcagt actgcaggac gtgatcatag tcccccacga cgaacgccgt
     6061 tcccacgggg cgcccctccc gtccttcccg cgctatgcct tcggcgatct ggagcacctt
     6121 ttccagcacc tgcggctcca cgtcccccag cggagccacg gccggccgga cgaatacggt
     6181 cgggaactcc cgacccacgt cgatgaccag gagggtatcc agcgtgccgg aggacggaac
     6241 accggagagg ctcaccacgg tgtctccccg ggcgagcagg ccccgagaga tggcgaagag
     6301 aagcgccagg tccacctgcc cgtgtcggtc cagggtatgg aacgggacct ggagccgatg
     6361 gatcccgggg ccatcctggg gcgtcggctc ctgcttggag gaggacacca ggatgagctg
     6421 gccctgggcg ggcagatcct cgatgaagtg aaggctgcct acggcgtccg tgtggaggac
     6481 caccgccttt gccttgacct cttccgccac cgagagggcg tgggcaaaga ggagccgcga
     6541 aagccggagc ttgggggacg atggcccctg gtcctgggac acgtgcccgc gcatggtcag
     6601 gagccgatag ctctccagcg gcgtgcgcgc cgtcaagacc agctgcatga gcgcccgatc
     6661 gtgcagggtg cgggctatct ccgccaacag gagcacgtga cggtccggct cgttcacccc
     6721 gctcacgatg aggatcagga ggttcaccgg gtttccgtcg ctggagtccc agcggatccc
     6781 gcggcggctt cggcccacgg ccacctggat gccctcccag ttgggaagct tgacgtgggg
     6841 gatggcgacg ccgggagcga tccaggaacc cagcacccgc tcgcgctcgc ggatgacgcg
     6901 caggaggccg tcccgttccc gtggcccgcc cagggagagc gtcgttgcca gctcgcgcag
     6961 ggcgccgtcc ttggtgcggc ttcgcagggt cacgatgcgc ttggggctga gcgtgctgga
     7021 aaggatggtc acgtcggctt cctaacagtg aagtatagtg cgccatcggc ccggattgcc
     7081 aacccggaag cccgcggcct tcccgggcct tcccgtccgc tggccgccgc tgatattatc
     7141 gtaggaagag gtttttcccc atggcgattg agcgcgagcc gctggattcc ttccagagcg
     7201 ataccggtgt ccagcagaag cccaggcttg aggagccccg tctctaccgg gtcatcctgc
     7261 acaacgacaa ctacaccacc atggatttcg tggtgaaggt gatcgtggaa gtgttccaca
     7321 agccggcggc ggaggcgacc aagatcatgc tggatgtgca tcgggcggga cgggggatcg
     7381 tgggggtcta taccctggac atcgccacca ccaaggtctc ccaggtccac gccatggccc
     7441 gtcagaacga gttccccctg cgcgcaagct gcgaagaggc ctgaatggag atctccaccg
     7501 agctgaacca cgtaatcatg gccgcgtacc gggaagccga atcccgcagc cacgagtaca
     7561 tgaccccgga gcacgtgctg tacgcgtctc tcttcttcga ccgcggcaga gaggtcatca
     7621 ccggctgcgg agggaacgtg gacaacctcc tgaaggacct ggaggagttc ttccgcaacc
     7681 gggtccccgt ggtggagggc tcgaagccca tccagtccgc ggggttccag tccatgatgg
     7741 agaatgccat cctgcacacg gcatccgcgg agaagcaggc cgtggaggtg gatgacatgc
     7801 tggcctccct cctggaggag aaggagtcct tcgcggtgca tctgctggcc cgccaggggg
     7861 tggatcggct ctcgctcctc tccttcatct cccacggcgt caccgcctac ccggtaaccg
     7921 aggagcccgc cgatggcgat gcggccgagg accgggagga ggaagggacc gggcaggagg
     7981 ggtcggcgag cgccaccccc aaggccgggc gggcgctcca ggcgttcact accgacctga
     8041 cggagaaggc ccgctccggc gggatcgacc cgctcatcgg tcgggaggag atcctccagc
     8101 gcaccatcca ggtcctgagc cgccggctca agaacaaccc gatccacgtg ggggagcccg
     8161 gcgtcggcaa gaccgcgatc acggagggcc tggcgcagcg gatcgtggac ggcaaggtgc
     8221 ccaaggccct gaaggacttc cggatctacg ccctggacat gggcgcgctc ctggcgggaa
     8281 ccaagttccg cggtgacttc gaggagagac tgaagcgcgt gatcaaggag ctggagcgcc
     8341 agcagcgcgc cattctgttc atcgacgaga tccacaccgt ggtgggggcc gggtcggttt
     8401 cgggggggtc gctggacgcg tccaacatcc tgaagccggt gctctcctcc ggcaaggtgc
     8461 gctgcatcgg ctccaccacg tacgaggact accgcaagta cttcgacaag gaccgcgccc
     8521 tgtcccggcg gttccagaag gtggaggtgc ccgagcccac cgcggaggag agccgccaga
     8581 tccttctggg gctgcggtcg aagtacgagg agcaccacgg ggtgcgctac accgatgagg
     8641 cgatcgccgc ggcggcggag ctctccgccc ggttcatcac ggatcggcac cttccggaca
     8701 aggccataga cgtcctggac gagtccggcg cctgggtggc gctctacgga cccgaggtcg
     8761 agggaccccg ccccgtggag gtgggggtca tcgagcaggt ggtcgcgcgc attgcccgca
     8821 tcccccaggc cagcgtgtcc tcggacgaac gccggaagct gcggtcgctg gaggcggacc
     8881 tccgtcagcg gatcttcggt caggaccacg ccatcgacgt cgtggtgcgc gccatccgca
     8941 agtcccgcgc ggggttcggg gagcgggaga agccggtcgc ctcactcctg ttcgtcggac
     9001 cgaccggcgt cggcaagacc gagctctccc ggcagatcgc ccagtccctg ggcatcggcc
     9061 tcctgcgctt cgacatgagc gaataccagg agcgccacac ggtgtcccgc ctcatcggcg
     9121 cccctcccgg gtacgtcggc tacgacgacg gcgggctgct cacggacgcc atccgcaaac
     9181 agccccacgc ggtgctcctg ctggacgaga tcgagaaggc gcaccaggac atctacaaca
     9241 tgctcctgca gatcatggac tacgccacgc tcaccgataa caatggaaag aaagcggact
     9301 tccgcaacat catcctcatc atgacctcca acgccggggc ccgggagatc ggcaaggccc
     9361 tggtcggatt cggggagcgc tcggtgaagg aggaggccgt cctggacgcc gtggagcgga
     9421 tcttcgcgcc ggagttcagg aaccgtttgg acgcggtggt ccggttcaac gccctggggc
     9481 acgagcaggt gttggccgtc gtggacaagc agctccggga gttccagcgg gagctggaag
     9541 ccaagggcgt gagcctggag gtgagcgacg cctgccggga gtggctggct cgcaaggggt
     9601 actcggagga gttcggcgcg cgagagatcg cccggctgat ctcctcgtcc atcaaggact
     9661 tcttcgtgga tgagatcctc ttcggccggc tgaccggcgg cgggaagacc cgagcggacc
     9721 tcgatggaga gaaggtggtg atcacggttc tatgaacgtc gtggccgcgt tcctgaccca
     9781 gaccacgatc gacgtcccgg tgacggtctt ccgcctggtg ctcagcttcg tcatgggcgc
     9841 ggcgatcggg ctggagcggg agtggcaccg acagtccgcg ggattgcgga cccacatcct
     9901 gatcagtctc ggcgccacga tgctcaccct tctttccatc tacatcccac aagagttcac
     9961 gcgttaccag ggaatcgatc cgggccgcat cgcggcccag gtcatctccg gcatcgggtt
    10021 cctgggcggt ggcgcgatct tccggctcgg cgtgaacgtc cgagggctga ccacggccgc
    10081 ctcgatctgg gtggtggccg gcatcgggat gctggtcggg gcgggcatgt acttcgcgtc
    10141 cctggcaggg accggactgg tcctgctggc gctcatcgtc ctggtgccgg agggcatccg
    10201 gtggaacggg ctctacgaga aggtgggccg cgtttccggg atcacccaca tcagcgtgga
    10261 tcagaaagtt tagagcgttg ccttcacgac ggggccggtg ccggcgggcg cactatgccg
    10321 gccagcggcc ctccagggtg atggcctcgg aaagacgcct caggtattcg acgcggggaa
    10381 tctcgatggc gc
//
LOCUS       DASRZS010000009        13546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3852257, whole genome shotgun sequence.
ACCESSION   DASRZS010000009 DASRZS010000000
VERSION     DASRZS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 13546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13546
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3852257"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFH83_00175"
     CDS             complement(<1..269)
                     /locus_tag="VFH83_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484799.1"
                     /translation="MKPRSILPVGLAALTFVLLGGCPTPTPTVTAPPAENHYLSGRVV
                     EVQTGAGVAGLTVDFAGQSATTDSNGEFKFDLGNAGGVQNGSFCV"
     gene            complement(440..796)
                     /locus_tag="VFH83_00180"
     CDS             complement(440..796)
                     /locus_tag="VFH83_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6484800.1"
                     /translation="MADRNVAQSTRWLRPRADVVESKDGRLRVVLEMPGVRKEDLEVR
                     IENNELRILGKRQEPSQGKYLLRERPTGSYRQSYTLDETVDQSKIDASLEKGVLTVVL
                     DLKEQVKPRTIQVRGE"
     gene            complement(808..1263)
                     /locus_tag="VFH83_00185"
     CDS             complement(808..1263)
                     /locus_tag="VFH83_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6484801.1"
                     /translation="MDLLHWEDGSRDPFQEFEALQNEINRLFDVTRVPDPRGIFEGPL
                     SPAADLIETPSTFEVLCDLPGVEIGDIEISLAGNVLTIKGEKKRGSNPGDGHSYREEM
                     RSGRFQKTLQLPLPVDGSKTDAALKEGVLQVVLPKQEEHKPRQISVKVK"
     gene            1602..2075
                     /locus_tag="VFH83_00190"
     CDS             1602..2075
                     /locus_tag="VFH83_00190"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6484802.1"
                     /translation="MTETERIIDQLERAQHGDAWHGPSLRELLEGVTAAQAAAHPIPQ
                     AHSIWELVNHIIAWEQIALRRLRGELLVAITDEMSFPPIADTGAPSWQAALRALDESN
                     QSLRDAIRRIGDVQLNATVPGTPYTNYVLLHGVIQHDLYHGGQIAVLRKFSVPNP"
     gene            complement(2107..2742)
                     /locus_tag="VFH83_00195"
     CDS             complement(2107..2742)
                     /locus_tag="VFH83_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HET6484803.1"
                     /translation="MIPASSIAVFLLAALGLLLVPGPAVIYIVTRSVSQGRRAGIASV
                     MGIEVASLCHSVAAALGLSAILLTSSLVFSVVKYLGAAYLIYLGVRAIFSRQELPEVE
                     EGTPANLPQLFRKGLMVNLLNPKTALFFYAFLPQFVSPARGSTVVQILTLGILFVILA
                     TITDSTYALVSSGVGRFVITRPGFRRVQRYVTGGVYMALGVTAAFTGSGKK"
     gene            complement(2842..3819)
                     /locus_tag="VFH83_00200"
     CDS             complement(2842..3819)
                     /locus_tag="VFH83_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HET6484804.1"
                     /translation="MTALVAAVPLSAPSPWIPEGVPAPGGQDRPIFKEIWGYVLQGEE
                     KELSGAEPITDIGYFGVNLTNTGRITQTVARPAITLKDGSKPRVHLVIAELSNYALMH
                     FSLDPQYGVMPLLVQDICRLSAAFDGVQIDFEAVSRDDADSFIDFLRQLRTSLPFGKV
                     LSVAVPARTQTISDAYDYTRIAPLVDRLVIMAYDEHWSTSSPGPVASLPWCSKVADYA
                     RGAVETGKIIMGLPLYGRAWQDKKLARALRFESVQDLVTQTNSVPAYAPELGAHFEYS
                     ENVVVSVFYDDERTILDKLQLYRARGIESVSFWRVGLGPPQLWELMDHT"
     gene            complement(4037..6322)
                     /locus_tag="VFH83_00205"
     CDS             complement(4037..6322)
                     /locus_tag="VFH83_00205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6484805.1"
                     /translation="MKELTPLKRAQSLNRLLLNSFFGAAYGPIIGVGGAVFTGYALWM
                     GLQASEIALMTSVALFAGLIFPFSAVIVGAVKRKKPFLIFAGFGETCFLVSISLVPLL
                     ARGTTGRLLLLAAFVLASTLLANSLGPTFSSWVSTIIPEDTRARFMSRQSVIGTITGM
                     AVSFAVGQYIDRMGGHSYLSFLLPFAFALVVGLGRYWVYLQIPFPQALEHDDRKLSPL
                     RSMGAPLQHREFRRLLVFYLMVAIGGSLGDPFYSVYMLQRLKVSYSFIALLTIIASLV
                     AILTWLAWGRISQRFGNKPVLWVSVVCRCAMPLLWLFATPSSYASVLIGINVLSAIMG
                     PGFGIAITTLQYELVPEGPARSAYFASWAFITSIAGVVTTLTGALLANLLSPLTFSIA
                     GIGFDNLKVIFVLSGLLLVVPFVLMRGLPDVRIRGRAIALRQVFRGNPMAYLFNAMVL
                     SLLKDTSVRARAIEGMGHSRSPMAMDTLVQYLADPNPHIRQAAVKALGGTRSDEAVSL
                     LSAELANEESPIRIEAAESLGRLGDPSGVAPLAQAMDSSDARLAAVAARALGDIGGAE
                     IADLLFERLRATPAGNKILFLALVEALSKLGDSRVIRPAIDCLQGFSSPINRLQILNA
                     VCRTVGADNDFYTMLGQERLRLAQRLYKIQKALRRYLPKLVPLRRLLAVDAIDDLAHA
                     IEEEDYDRIPELALFLADTVEWRKPEPHPGLLALRCYVEAQRKGVCERPEVFSFVILE
                     RVFAHAFAGSSLGRASTPTRR"
     gene            complement(6461..7534)
                     /locus_tag="VFH83_00210"
     CDS             complement(6461..7534)
                     /locus_tag="VFH83_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET6484806.1"
                     /translation="MEAASMAATRMKAAVYEGIERIVVRELPVPDIGEDELLVQIKTC
                     AICGTDVRTFHHGSHMVTPPLVIGHELAGVVVRAGRRVTGFSEGQAVTMESSIPCLSC
                     PACARGRYNICDALKPIGFHYPGGFAQYMRVPAQGLVAQCVLPIPAGLPFDQACLSEP
                     FACAINGQELTRIEEGDTVVILGAGPLGSMHAELARINGAGRVILCDRLPERLALVTK
                     DIRADRFVDLSREDLKEVVFGLTAGRGADVVIVAAPSAEAQAAAIEVAAPRGRVNLFG
                     GLPRTNPVASLNANLIHYRELYVNGSFGSTARQQARALELFASGRIQVGKFVSARFPL
                     ERILDGFAAAESKTGYRVVIGME"
     gene            complement(7560..9428)
                     /locus_tag="VFH83_00215"
     CDS             complement(7560..9428)
                     /locus_tag="VFH83_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET6484807.1"
                     /translation="MGFVHFDSDGLLIGPSGEPRYVVGINYVASYICTNFWEDFRPAA
                     IEKDLDGIQALGLNAVRIPVHWGFAEPEIGVYSEIFFQRFDAFLDMAGRRGLLVMPWF
                     LVGIATKSYDFPFRNGRSFFTGEMLDIEERHLKAITGRYRNDERILMWDICDEPEYFS
                     FNEYSEQLPYKRPVMARWVKTLADAIRSVDANHLVTLGYGAIATQHFGIHVRDAAEAL
                     DLMVVTAYPGPSHEQIDRYRNNYFLPYNVRFNTLGKPVLSCEAPGSTSVAFSEEVIGN
                     YFRVALYGNLLAGASGALPWVYNDFQREIWHEKPLEEATFEPSFGIVTTDGRLKPSGA
                     ALAEFARFANEQGIGSCRPPAARAAILVPRRYYEEIEPAYKKLYTTFILAKASVDAID
                     FLWEDQDPSRYRLVLIPSTRGMTTSAWDRLRRFVEAGGTILCIHDDRRGLSAYFNDLF
                     GVETITTEKDHGYEGMVAREAWGGFHRGERFAFAPNSRSEILRVQARSARVLFAFEDG
                     LPAFVVNGLGRGKAFLATRPLEDGLLDCRYEEFLESRCFDLHAALTEEAGVSPEFSCP
                     DRRVEVGALEKRGTDERLIVCINHDTSAVSTRLLVRGEERALELAPAGVKVFTVER"
     gene            complement(9491..10948)
                     /locus_tag="VFH83_00220"
     CDS             complement(9491..10948)
                     /locus_tag="VFH83_00220"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HET6484808.1"
                     /translation="MARVRFTISGGMTEEQLQLLHTRALDVLATVGLEVNSPATLELL
                     REKAGYRIQGRRVLVDRALVEECVRLCRASRRETGGSREAAPAAGEGPQAASGAVSRR
                     RLLEILTAYPTHFVDWRDASMKPIAESDLIPMARLVDALYDRGVRGGAPGVPQDVPPR
                     VRGIHAYRIGAEYCRFGGSAPATNERDIQWLYRMDEVMGQPFGLGIFIVNPLRIEGNT
                     LDQLFSLRGRKFPVLVGSMPQMGVSAPVTLLGAFVVGIAAAWGAYAVVREITGTDDIG
                     VECRVWPTSARSLEMAYGSPEMVLGDLVLNQLRRFYNWNTPDADPFHSASLLPDAQAS
                     AQRAAFGMAMALAGNRSFRFGGLLGTDLVFSPEQLLLDVETLRYLDCVASGFEFSEEA
                     FGMDAIRRVGPAGSYLDDEPTLKRLSGELWQPEVWRMEPLGSWMGGRSAAPGVSDAVR
                     EQVQPLIDGQTYRLDGTRARALDALVSAAERDLLG"
     gene            complement(11006..11905)
                     /locus_tag="VFH83_00225"
     CDS             complement(11006..11905)
                     /locus_tag="VFH83_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6484809.1"
                     /translation="MSGPNRAGGAEGVSRQGLSVVDSHVHLWDTGLLSYSWLSRNPTI
                     NRPHLLADYARATSGFRIDGMVFVQAEVDVSQAMDEARWVVGLARQEPRLKGVVAWAP
                     LESGDACRPALEKLASSPLVKGIRRIIEFEPSLDFCLQSGFVKGVRMLPEFGLRFDIC
                     IKPHQMANTMALMRQCPEVPFILDHIAKPDIRGGMWEPWASRMREMASFPRCRCKISG
                     VITEADPARWTVEGIRPYILHAIECFGPDRVLFGGDWPVVVPSARYDQWMDALLRVAS
                     ELTEEERRKLFRDNAIQAYGLDL"
     gene            12058..12417
                     /locus_tag="VFH83_00230"
     CDS             12058..12417
                     /locus_tag="VFH83_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6484810.1"
                     /translation="MRILIADDQRHTRSGLRALLLASLPSADIWEASTGLEAVRLTDE
                     VQPDLILMDIRMPELDGLSATRRIKAVHGNVKVLVLSLRSGATAEAAAAGADAFISKS
                     ENPERLLGAVLALLPPA"
     gene            12522..13199
                     /locus_tag="VFH83_00235"
     CDS             12522..13199
                     /locus_tag="VFH83_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HET6484811.1"
                     /translation="MEESLKLYLSEVQKVPLLSADQERSLGRAARLGDAAAGQRLVQA
                     NLRFVVSVARQLQHRGLPLEDLVNEGNIGLMKAAQRFDPDRGVRFITYAVWWIRQAML
                     RAIKAQGKVPAIPVDEALEAGEAAIEDVSLPSPDEELARSLMKAEIATALTELPERER
                     KILDERYGLSSRPPSTLAQMGESCHLSRERIRQLEKRALRRLRAKGSVRALAPSPLAM
                     EEGVCVA"
     gene            13262..>13546
                     /locus_tag="VFH83_00240"
     CDS             13262..>13546
                     /locus_tag="VFH83_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484812.1"
                     /translation="MPTPLALFVDDPCPLVHVYRFHKEHVHGEPPTTRDGRPLLDEIP
                     NDFLDAFCDVVQRFGMRGKFSIVPAPAWRGDLRSGINGDLSATRWWVDQAK"
BASE COUNT         2436 a         4577 c         4342 g         2191 t
ORIGIN      
        1 tggacacaga agctgccgtt ctggaccccg cccgcgttgc caagatcgaa cttgaactct
       61 ccgttcgaat ccgtcgtggc tgactggccg gcgaaatcga ccgtcaagcc tgcaacccct
      121 gcacccgtct gcacctcgac aactctgcct gagaggtagt ggttttccgc gggtggagct
      181 gtgaccgttg gcgtaggagt cgggcatccg cccagaagaa cgaacgtcag ggccgcaagg
      241 cccaccggaa ggattgatcg tggcttcact tttcacctcc acatgctgac tggcacgatt
      301 cggttgagag ctatcctata aatctatctc tcggaaaagc gcatatccgt gcgatgcaga
      361 ttgcccgcgg ataagcaagc gcagctttgc agagaaggcg gctggcgggc ctcacggccc
      421 gccacccccg gcacaattct tactcgcccc gaacctgaat ggtccgtggc ttcacctgtt
      481 ccttgaggtc cagaacaacc gtcaggacgc ccttctccag gctcgcgtcg atcttcgact
      541 ggtcgacggt ttcatctaga gtgtagctct ggcggtagct gcccgtgggc ctctctcgaa
      601 ggaggtactt gccttgggaa ggctcctgtc gcttcccgag gatccgaagc tcgttgttct
      661 cgatccggac ctccagatcc tccttccgca cgcccggcat ctccagtacc acccgaagcc
      721 tgccgtcctt ggactccacg acatccgccc ttggcctcaa ccagcgggtc gactgagcaa
      781 cgttcctgtc cgccatgacg cgcctcctca cttcaccttc accgagatct ggcgaggctt
      841 gtgctcctcc tgcttgggca gcaccacctg cagcacgcct tccttcaacg ccgcatccgt
      901 cttgcttcca tcgaccggca gcgggagctg cagggtcttc tggaaccttc cggacctcat
      961 ctcttcgcgg tagctgtgcc cgtccccggg attcgaaccg cgtttcttct cccctttgat
     1021 ggtaagcacg ttgcccgcga gggagatctc gatgtcgccg atctcgactc caggcaagtc
     1081 gcagagcacc tcgaaggtgc tcggggtttc gatcagatcc gcggccggag aaagagggcc
     1141 ctcgaagatg cctcgcgggt ccggaaccct ggtgacgtcg aagagccggt tgatctcgtt
     1201 ctgcagcgct tcgaactcct ggaacgggtc tctcgatccg tcctcccagt gaagcagatc
     1261 catatgacac ctcctgaaag agattgtgtt cacccggata aaagcaaggg acgtgccaac
     1321 gtgcaccacc ggagacccgc ctgggctaat tatctataag gcaaaggaat agccgctcac
     1381 tacccttcgg tttcccccac cacggcagtc ccggtggctg tggcgttctg ggacggcaac
     1441 cattggaggc aactgcccgg gtgtttccaa atgctacaga cgcggatgtc cgcttccgct
     1501 ctctggccca gttttgccca cgttgctttt cccacctggc ggaagtaccc tcggctctgc
     1561 cagtgccccc gaggcgtcgg gacaggaacc aggaagacgc tatgaccgag acggaaagaa
     1621 tcatcgatca gctcgaacgg gcccagcacg gcgacgcgtg gcacggcccc agtctgcggg
     1681 agctgctcga aggcgtgacc gccgcacagg ccgctgccca cccgatcccc caggcccaca
     1741 gcatctggga gctcgtcaat cacatcatcg cctgggagca gatcgcgctg cgtaggctca
     1801 ggggcgagct cctggtcgca atcaccgatg agatgagttt cccgccgatc gccgatacgg
     1861 gagccccgtc ctggcaggct gctctgcggg cgctggacga gagcaatcag tcgctgcggg
     1921 acgccatccg tcggattggc gatgtccagc tgaacgcgac cgtgcccggg accccgtata
     1981 cgaactacgt tctgcttcac ggcgtgatcc agcacgatct ctatcatggc ggccagatcg
     2041 ccgtgctgag gaagttctcc gtcccaaacc cgtaaagggg ttacgggcag gcccgctccg
     2101 tcgtcgctac ttcttccccg agccggtgaa cgcggcggtc accccgagag ccatgtagac
     2161 tccgccggtg acgtatcgct ggactctccg aaagcccggc cgcgttatca cgaaccttcc
     2221 gactccggag ctcaccaggg cgtacgtgct gtccgtaatg gtagccagaa tcacgaaaag
     2281 gatcccgagg gtcaaaatct gcaccacggt agacccacga gcgggactga cgaactgcgg
     2341 caggaacgcg tagaagaaca gcgccgtctt cggattgagg aggttcacca tgaggccctt
     2401 gcggaatagc tgcgggaggt tcgccggcgt cccttcctca acctcgggaa gctcctgtct
     2461 cgagaagatt gcccttactc cgagatagat caggtacgcc gccccaaggt acttgaccac
     2521 gctgaatacc agtgaggatg tcagcaggat tgcggacagc cccaatgcgg cagcaacgga
     2581 atgacaaaga ctggccactt cgatgcccat gaccgacgca ataccggccc ttcgtccctg
     2641 ggagacactc cgcgtcacga tgtagatgac cgcgggcccg gggaccagga gaagcccaag
     2701 ggccgcgagc agaaacacgg cgatggaaga ggctgggatc aacggtcacc gcccatgaag
     2761 ggcagtctat ccgggatcgg cgcgatgatc cacgtagcga ccgaccccgc cgaccccgtg
     2821 ggtgtgacgc gccgcagccg cctaggtatg gtccatgagc tcccagagct gcggaggccc
     2881 caggccgacg cgccagaagg aaaccgactc gatcccgcgc gcccggtaga gctgcagttt
     2941 gtccaggatg gtgcgctcgt cgtcgtagaa cacggagacg accacgttct ccgaatactc
     3001 gaagtgagcg ccgagctcgg gagcgtaggc cgggacgctg ttggtctggg tcacgaggtc
     3061 ctgcacgctc tcgaagcgaa gcgcgcgggc cagctttttg tcctgccagg ccctcccgta
     3121 cagcgggagg cccatgatga tcttcccggt ttccaccgcg cccctggcgt agtccgccac
     3181 cttggagcac caggggagcg aggctacggg ccccggggag ctcgtgctcc agtgctcgtc
     3241 gtaggccatg atgaccaggc gatccaccag gggggcgatc cgggtgtagt cgtaggcgtc
     3301 ggagatcgtc tgggtccgcg cggggacggc gacgctcagg accttgccga acggcaggga
     3361 ggtccgaagc tgccgaagga agtcgatgaa ggaatcggca tcgtccctcg acaccgcctc
     3421 gaagtcgatc tgcaccccgt cgaaggccgc ggagagccga cagatatcct gcaccagcag
     3481 aggcatcacg ccatactggg gatccaagga gaagtgcatc aacgcatagt tcgagagctc
     3541 ggcgatgacc aggtggaccc ggggtttgga cccgtccttc agggttatcg cgggacgggc
     3601 taccgtctgg gtgatccgcc cggtgttcgt caggttcact ccgaagtacc cgatatccgt
     3661 gatgggctcg gcgccgctca gctccttctc ctccccctgc aggacatagc cccagatttc
     3721 cttgaagatc ggccgatcct gtccgccggg agcgggaacg ccttcgggaa tccagggaga
     3781 gggagcggag aggggtacgg cggccaccag agcggtcacc accgccccca ctcgcaatcc
     3841 acgggcccga cgcccgaccc gctgccgcca accaaagagc cgcatgagaa tctccagtct
     3901 ttcgaacacg gtagggctgc cgctcaggca ccccagctca tcgaccaggg gccagaccag
     3961 ccctccagat caatggtatc ccgccgggcg gcgaatccat cgcttccgat ctcgcggatt
     4021 cccgctgact ccgtccctat cggcgcgtcg gcgttgaggc gcgacccagg gatgacccgg
     4081 cgaacgcatg ggcgaacacg cgctccagga tcacgaagga gaagacctcc ggccgctcgc
     4141 acacgccttt ccgctgggct tccacgtagc agcgcaaggc caggaggccg gggtgcggct
     4201 cgggctttcg ccactccacg gtatccgcca ggaacagggc gagctcgggg atgcgatcgt
     4261 agtcttcctc ttcgatggcg tgcgcaagat cgtcgatggc gtccacggcc aggagccggc
     4321 gcagcgggac gagcttgggg aggtaccggc gcagcgcctt ctggatcttg tacaggcgct
     4381 gggccagccg gaggcgctcc tgacccagca tcgtgtaaaa atcgttgtcc gcgcccacgg
     4441 tccgacaaac ggcattcagg atctgcagtc ggttgatcgg agagctgaac ccctgcagac
     4501 agtcgatggc cggccgtatc acccgggagt cccccagctt gctcagcgcc tccaccaggg
     4561 ccagaaagag aatcttgttc ccggcggggg tggcccgaag gcgctcgaag agcaggtccg
     4621 ctatctccgc tccaccgatg tcccccaggg cccgggccgc caccgcagcc agccgggcgt
     4681 cggaggagtc catggcctgc gccagagggg cgaccccgct ggggtctccg agccgtccca
     4741 gggactcggc cgcctctatg cgaatggggg actcttcgtt cgccagctcc gccgacaaga
     4801 gggagaccgc ctcgtccgag cgggtccccc ccagcgcctt gaccgccgcc tggcggatgt
     4861 gtgggttcgg gtccgcgagg tactggacca acgtgtccat tgccatgggg cttcgggaat
     4921 gccccatgcc ctcgatggct cgggcccgca cgctcgtatc cttgagcaga gacagcacca
     4981 tggcgttgaa gaggtaggcc atggggtttc cccggaagac ctgccggagg gcaatggcgc
     5041 ggccccggat cctcacgtcc ggcaatccgc gcatcagaac gaagggcacc accagcagca
     5101 gcccggagag cacgaagatc accttgaggt tgtcgaaccc gatccccgcg atggagaagg
     5161 tcagggggct gagcaggttg gccagcagcg cgccggtcag ggtggtgacc acgccggcga
     5221 tgctggtgat gaacgcccag cttgcgaagt aggccgagcg cgcgggcccc tcgggcacca
     5281 gctcgtattg cagcgtcgtg atcgcgatgc cgaaccccgg tcccatgatg gcgctcagca
     5341 cgttgatgcc gatcaagacc gacgcgtacg acgacggagt ggcgaacaac cacaggaggg
     5401 gcatggcgca gcgacagacc acgctcaccc agagcacggg cttgttcccg aaccgctggg
     5461 agatcctgcc ccacgccagc caggtcagga tggccaccag ggaggcgatg atggtaagca
     5521 acgcgatgaa cgagtagctc accttcagtc gctggagcat gtacacgctg tagaaggggt
     5581 ctcccaggga gccgcctatg gccaccatga gatagaagac caggaggcgt cgaaactccc
     5641 ggtgctgaag gggcgctccc atggacctca gggggctcag cttgcggtca tcgtgctcca
     5701 gcgcctgggg gaacgggatc tggaggtaca cccagtaccg gcccagaccc accaccaggg
     5761 cgaatgcgaa cggcagcagg aagctgaggt agctgtgccc gcccatccgg tcgatgtact
     5821 ggcccaccgc gaaggagacg gccattccgg tgatcgtccc gatgacgctc tgccggctca
     5881 tgaacctcgc tcgggtgtcc tcgggaatga tcgtggatac ccagctcgag aaggtgggac
     5941 cgagggaatt ggccagcagc gtggaggcca gcacgaaggc ggccagcagg aggaggcgac
     6001 ccgtggttcc gcgggccaga agcgggacca acgagatgga gaccaggaaa caggtctcac
     6061 caaatccggc gaagatcagg aacggtttct tccgtttgac cgcgcctacg atcaccgcgg
     6121 aaaagggaaa gatgaggccg gcgaacagag caacggaggt catcagggcg atctccgatg
     6181 cctgcagccc catccacaag gcgtaccccg tgaagaccgc tccgccaacg cctatgatcg
     6241 gcccgtaggc ggcgccgaaa aaggagttca gcaggagccg gttcagggac tgggcgcgct
     6301 tgagcggtgt cagttccttc atgacggttc gccgccgtcg gcgttgctac ctccgggtga
     6361 catgaaagat atcggcagga ggacggctgt caaggagagc gggccgctct ccgagcgccg
     6421 ccgctgcagc tcggagtcca ggccggaccc cggcggcaga tcattccatg ccgatcacga
     6481 cccgatatcc cgtcttgctc tcggcggcgg caaacccatc caggatccgc tccagaggga
     6541 accgggccga gacgaacttc ccgacctgga tccttccgga tgcgaaaagc tccagggcgc
     6601 gcgcctgctg tcgggcggtc gaaccgaagg agccgttgac gtagagctcc cggtagtgga
     6661 tgaggttcgc gttcaggctg gccacggggt tcgtcctggg cagcccgccg aagaggttca
     6721 cccggcctcg tggggccgcc acctcgatgg ctgccgcctg ggcctcggcg ctcggcgcgg
     6781 caacgatgac cacatccgcg ccgcggccgg ccgtgagccc gaagaccacc tccttgaggt
     6841 cctctcggga gaggtcgacg aaccggtcgg cccggatgtc cttcgtgacg agggcaagcc
     6901 gttccggcag ccggtcgcac aggatcaccc ggcccgcgcc gttgatccga gccaactccg
     6961 catgcatgct gcccagcggt cccgcgccca ggattaccac ggtgtctccc tcctcgatgc
     7021 gcgtcaactc ctggccgttg atcgcgcagg cgaacggctc cgaaaggcac gcctggtcga
     7081 aggggagacc cgcggggatg ggcagcacgc actgcgcgac gaggccctgc gccggcaccc
     7141 tcatgtactg ggcaaaccca ccgggatagt ggaacccgat cggcttgagg gcatcgcaga
     7201 tattgtaccg ccccctggcg caggccgggc agctcagaca cgggatgctg gactccatcg
     7261 tcaccgcctg tccctcggag aacccggtga cgcgcctccc cgcgcggacc acgacgccgg
     7321 ccagctcgtg accgatgacg agcggcgggg tgaccatgtg cgatccgtgg tggaaggtac
     7381 ggacgtcggt gccgcagatc gcgcaggtct tgatctggac caaaagctcg tcctctccga
     7441 tgtcgggaac gggaagctcc cgcaccacga tccgctcgat cccctcatag accgccgcct
     7501 tcatccgtgt cgccgccatc gatgctgcct ccattcccac gctccccctc gcgcttccct
     7561 tatcgctcca ccgtgaacac cttcaccccc gcgggagcca gctccagggc gcgctcctcg
     7621 cctcggacaa gcaggcgggt ggagaccgcg ctggtgtcgt ggttgatgca gacgatcagc
     7681 cgctcgtcgg tcccccgttt ctccagcgcc ccaacctcca cccgtcgatc cggacaggag
     7741 aactccggcg agactcccgc ctcttcggtg agagcggcgt gcagatcgaa gcagcggctc
     7801 tccaggaact cctcgtagcg gcagtccagg agcccatcct ccagcgggcg ggtggccagg
     7861 aaggccttgc cccggcccag gccgttgacc acaaaggcgg gcaggccgtc ctcgaaggcg
     7921 aagagcaccc gggcagaccg ggcctgaacg cgcaggatct ccgaacggct gttcggcgca
     7981 aacgcgaacc gctccccgcg atggaaccct ccccacgcct cgcgagccac catgccctcg
     8041 tacccgtggt ccttctcggt ggtaatggtc tccacgccga aaaggtcgtt gaaataggcg
     8101 gagaggccgc ggcggtcgtc gtggatgcag aggatcgttc cgccggcctc cacgaaccgg
     8161 cgaagccggt cccaggcgct ggtggtcatg ccgcgggtgg acgggatcag caccaggcgg
     8221 taccgcgacg gatcctggtc ctcccagagg aagtcgatgg catccacgct ggccttggcc
     8281 aggatgaacg tggtgtacag cttcttgtac gcgggctcga tctcctcgta gtagcgccgg
     8341 ggaaccagga tggcggctcg cgccgcagga ggccggcagg agccgatccc ctgctcgttc
     8401 gcgaatcgcg cgaactccgc cagggctgct ccggagggct tgagccggcc gtccgtggtc
     8461 acgatgccga agctcggttc gaaggtggcc tcctccagcg gcttctcgtg ccagatctcc
     8521 cgctggaagt cgttgtagac ccagggcagg gcgccgctcg cgccggccag gaggttcccg
     8581 tagagggcca cgcggaagta gttcccgatc acctcctcgg agaaggcaac gctggtgctc
     8641 cccggggcct cacagctcag caccggcttc cccagcgtgt tgaaccgcac gttgtacggg
     8701 aggaagtagt tgttgcggta gcggtcgatc tgctcgtggg aggggccggg gtaagcggtc
     8761 accaccatca ggtccagcgc ctcggcggcg tcgcgcacgt ggatcccgaa atgctgcgtg
     8821 gcgatggcgc cgtagcccag cgtcaccagg tgattggcgt cgaccgagcg gatggcatcc
     8881 gccagcgtct tgacccatcg cgccatgacc gggcgcttgt acggcaactg ctcggagtac
     8941 tcgttgaagg agaagtactc cggctcgtcg cagatgtccc acatgaggat ccgctcgtcg
     9001 ttccgataac gcccggtgat cgccttgaga tggcgctcct cgatgtcgag catctccccg
     9061 gtgaagaagc ttcgaccgtt tcggaacggg aagtcatagg acttcgtggc gatccccacc
     9121 aggaaccagg gcatcacgag aagaccgcgc cgtcccgcca tgtccaggaa cgcgtcgaac
     9181 cgctggaaga agatctcgct gtagaccccg atctcgggct ccgcgaatcc ccagtgcacc
     9241 gggatgcgca cggcgttgag cccgagggcc tggatgccgt cgaggtcctt ctcgatggcg
     9301 gccggccgga agtcctccca gaagttggtg cagatgtagc tcgccacgta gttgatcccc
     9361 accacgtacc gtggctctcc ggaaggcccg atcaggagac cgtcggagtc gaagtgcacg
     9421 aatcccatgg aggctccttt ccctgccgcc gggcggttgc ggcggcccac cgtccctggt
     9481 cccgacgccc tcagcccagc aggtcgcgct ccgcggcgga caccagcgcg tccagggcac
     9541 gagcgcgagt cccatccagc cgatacgtct ggccgtcgat cagcggctgc acctgttcgc
     9601 ggaccgcgtc cgagaccccg ggcgccgcgg agcgcccgcc catccagctt cccagcggct
     9661 ccatgcgcca aacctccggc tgccacagct cgccggagag ccgcttgagg gtgggctcgt
     9721 cgtccagata cgagccggcg ggccccacgc ggcggatggc atccatgccg aacgcctcct
     9781 ccgagaactc gaaccccgag gcgacgcagt cgaggtaccg aagggtctcc acgtccagga
     9841 gcagctgctc cgggctgaac accaggtcag tccccaggag gccgccgaag cggaagctcc
     9901 gattgccggc gagcgccatg gccatgccga aggctgcccg ctgcgcggag gcctgggcgt
     9961 ccggcagaag cgacgcggag tggaaggggt ccgcgtccgg cgtgttccag ttgtagaatc
    10021 ggcgaagctg gttgagcacc aggtctccca gcaccatctc gggggacccg taggccatct
    10081 ccaggcttcg ggcgctggtg ggccacacac ggcactccac gccgatgtcg tcggtgccgg
    10141 tgatctcccg caccaccgcg taggctcccc acgccgccgc gatgccgacc acgaacgctc
    10201 ccagcagggt caccggcgcg gagacgccca tctgcggcat gctcccgacc aggaccggga
    10261 acttcctccc tcgcagcgag aagagctggt ccagggtgtt cccctcgata cgcagcgggt
    10321 tgacgatgaa gatccccagc ccgaagggct ggcccatgac ctcgtccatc cggtagagcc
    10381 actggatgtc gcgctcgttg gtggccggcg ccgacccgcc gaaccggcag tactcggcgc
    10441 cgatgcggta agcgtggatg ccccgcaccc tcggcggcac gtcctgcgga acccccggcg
    10501 cgccgccccg cacccctcgg tcgtacagcg catccaccag gcgcgccatc gggatgagat
    10561 cgctctccgc gatgggcttc atcgacgcgt ctcgccaatc cacgaaatgc gtgggatagg
    10621 cggtcaggat ctccaggagt cgccgccggc tgacggcccc cgatgccgct tggggaccct
    10681 cccccgcagc gggagccgcc tcgcgagagc ctccggtttc gcggcggctg gcgcggcaga
    10741 gacgtacgca ctcttcgacg agggcccgat ccacgagcac acgtcggccc tgaatccggt
    10801 accccgcctt ctcgcgcaag agctcgaggg ttgccgggct attcacctcc aggccgacgg
    10861 ttgcaagcac gtccagcgct cgagtatgca ggagctggag ctgctcctcg gtcatgcctc
    10921 cggagattgt gaaacggacc cgagccatgt cccgcgtagg ataatggccc gtcttcggcg
    10981 tgtatagcga ccgtcgatcc tgtggttaca ggtccaggcc gtacgcctgg attgcgttgt
    11041 cgcggaagag cttccgccgc tcctcctccg tgagctccga tgccacccgc aggagcgcgt
    11101 ccatccactg gtcgtagcgc gcggaaggga ccaccaccgg ccagtccccg ccgaagagga
    11161 cgcgatccgg gccaaagcac tcgatggcgt gcaggatgta cggacgaatt ccttccacgg
    11221 tccagcgcgc ggggtccgcc tcggtgatga ctccggagat cttgcagcga caccgcggga
    11281 agctcgccat ctcccgcata cgcgacgccc agggctccca catcccgccc cggatatcag
    11341 gcttggcgat gtggtccagg atgaacggga cctcggggca ctgccgcatc agggccatgg
    11401 tgttcgccat ctggtggggc ttgatgcaga tgtcgaagcg aagcccgaac tccgggagca
    11461 tccggacccc cttcacgaat ccggactgaa ggcagaagtc caggctcggc tcaaactcga
    11521 tgatccgccg gatgcccttc accagcggcg aggaggcgag cttctccagc gcgggccgac
    11581 acgcatcgcc ggattccagg ggcgcccagg caaccacgcc tttgagcctc ggctcctgcc
    11641 gggcaaggcc caccacccac cgtgcctcgt ccatcgcctg ggaaacgtcc acctccgctt
    11701 gcacgaagac catcccgtcg atgcggaacc ccgaggttgc ccgggcgtag tcggccagga
    11761 gatgcgggcg gttgatcgtc gggttcctgg aaagccacga gtagctgagg aggccggtgt
    11821 cccaaaggtg gacgtgggaa tccaccacgg aaagcccttg ccgggagaca ccctccgccc
    11881 cgcccgcgcg gttcggtcca ctcatgaagc cacctccttc ggttccctgg gcgcgctccg
    11941 gagagggtac ccaaaatccc tctttttgat gagggcgggt cggagggcgc ggcctatatt
    12001 ggtgaggtca gactgcaggc gggccgccgg gcccgccgtc atgggagcgt agccaatttg
    12061 agaatcctta tcgccgacga ccagagacat acccgatccg ggcttcgcgc cctgctcctg
    12121 gcgtcccttc cttccgccga tatctgggaa gcctccacgg gtctggaggc ggtccgcctc
    12181 accgacgagg tccagccgga cctcatcctg atggacatcc gtatgcccga gctggacgga
    12241 ctctccgcga cccgccgcat caaagcggtg cacggcaacg tcaaagtgct cgtgctgtcc
    12301 ctgcgcagcg gcgctaccgc ggaggccgcg gcagccggcg ccgatgcgtt catctccaag
    12361 agcgagaacc ccgaacggct cctgggcgcc gtcctggcgc tccttccgcc ggcatagcgc
    12421 cttcctcctc ccgccgcctc agcgcctccc tctttggtgg tagacccgat tccatcattc
    12481 caacgatgcg tccgcgggcc ctctcggggt attcaagggg catggaagag tccttgaagc
    12541 tatacctgag cgaggtccag aaggtcccgc tgctgagcgc cgaccaggaa cggtccctgg
    12601 gaagggctgc ccggctcggg gacgcggccg ccggccagag gctggtccag gccaacctgc
    12661 ggttcgtcgt ctcggtggcc cgacagcttc agcaccgcgg tcttcccctg gaggacctgg
    12721 tcaacgaagg aaacatcggt ctcatgaagg ccgctcagcg gttcgatccg gatcgaggcg
    12781 ttcgcttcat cacgtacgcc gtgtggtgga tccgccaggc catgctgagg gccatcaagg
    12841 cccagggcaa ggtccccgcg attcccgtcg atgaggcgct ggaagccggt gaggccgcca
    12901 tcgaagacgt ctcccttccc agccccgacg aggagctcgc ccgcagcctc atgaaggcgg
    12961 agatcgccac ggcgctcacc gagctacccg agcgggagcg caagatcctg gatgagcgct
    13021 acggcctctc ctcccgcccg ccgtccacgc tcgcccagat gggagagagc tgccacctca
    13081 gccgggagcg catccgtcag ctcgagaagc gggcgcttcg gcggcttcgc gccaaaggct
    13141 cggtccgagc cctggcgccg agccccttgg ccatggaaga gggggtgtgc gtggcgtgaa
    13201 cgggagcgcg cggtctgcca gcctgctggc ggcggaggta aaaggctggt agagtacgac
    13261 catgccgacg ccgctggcgc tctttgtcga tgatccctgc cccctggtgc acgtctatcg
    13321 gttccacaag gagcacgtgc acggggaacc ccccaccact cgagacggac ggccgcttct
    13381 ggatgagata cccaacgact tcctggacgc cttctgtgac gtcgtccaac ggttcgggat
    13441 gcgcggtaag ttctcgatcg ttcccgcgcc cgcatggaga ggggacctgc ggagcgggat
    13501 caacggagac ctctcggcga cccgatggtg ggtggaccag gcgaag
//
LOCUS       DASRZS010000010         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1160353, whole genome shotgun sequence.
ACCESSION   DASRZS010000010 DASRZS010000000
VERSION     DASRZS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160353"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VFH83_00245"
     CDS             <1..932
                     /locus_tag="VFH83_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6484813.1"
                     /translation="DMVGVGGWFDVMPNRGYEELVEETAEWEVKRNGAGAALKYWKHK
                     SGTPEHIDFLMANREIWTRDYRPKLLPFDPERLKIEETRKDLREAADAGRWTHYGHLF
                     IWELMRQSMGDVVMYESLLLDPDWIHDFARIYTDFFKTYFSCLIEQAGKPDGIWLYED
                     MGYKNGLFTSPKVLEKLIFPYYRELVSWFHEQGLPVILHSCGSTVDALDLIVEAGFDG
                     LNPMERKARGNDPLEFARRYGEKLVFVGGLDARVFESNDRARIEQEVSSYIDGMKGRG
                     ARLVFASDHSISPNTRYASYQWALEVYRKHMAY"
     gene            complement(964..1140)
                     /locus_tag="VFH83_00250"
     CDS             complement(964..1140)
                     /locus_tag="VFH83_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484814.1"
                     /translation="MPERIGDYMTRTGKMSQAQVDEVLRAKAAGDARHFGEIAVALHF
                     ITAADIEAFLTSQK"
     gene            1391..2032
                     /locus_tag="VFH83_00255"
     CDS             1391..2032
                     /locus_tag="VFH83_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HET6484815.1"
                     /translation="MGKADNVSGETVQFDAAATPQALERVAARLRERNIEVTIVSDAQ
                     QAREAVLQRIPAGAEVHAGKSKSLVDIGVTSVLAAEGAYTYLRNIFLKMDRNTQMREI
                     RKLISAPDFMLGSVAAITEDGVLVAASATASQLGPYASGAGKLILVVGSQKVVPDLEA
                     ALRRIREYVLPWEDAQVRPMVPSGSFVGKILLIEREWVAGRIEVILVEKPVGM"
BASE COUNT          414 a          672 c          748 g          378 t
ORIGIN      
        1 acgacatggt gggggtcggc ggctggttcg acgtgatgcc caaccgaggc tacgaggagc
       61 tggtggaaga gaccgcggaa tgggaagtga agcgcaacgg cgcgggggcg gccctgaagt
      121 actggaagca caagtccggc accccagagc acatcgactt cctcatggcg aaccgcgaga
      181 tctggacgag ggactatcgg cccaagctcc tgcccttcga cccggagcgc ctgaagatcg
      241 aggagacgcg caaggacctg cgggaggccg ccgacgcggg ccgctggacg cactacggtc
      301 acctgttcat ctgggagctg atgcgccaga gcatgggtga cgtggtcatg tacgagagcc
      361 tgctgctgga tcccgactgg atccacgact tcgcccggat ctacacggac ttcttcaaga
      421 cgtacttctc ctgcctcata gagcaggcgg gcaaaccgga cggcatctgg ctgtacgagg
      481 acatgggcta caagaacggt ctgttcacct ccccgaaagt gctggagaag ctcatcttcc
      541 cgtactaccg cgagctggtc agctggttcc atgagcaggg gctacccgtg atcctgcata
      601 gctgcggcag cacggtggat gccctggacc tgatcgtcga ggccgggttc gatggcctca
      661 accccatgga gcgcaaggcg cggggcaatg acccgctgga gttcgcccgc cgatacggcg
      721 agaagctggt tttcgtaggg ggcctggatg cgcgggtctt cgaatccaac gaccgcgcgc
      781 ggatcgagca ggaggtatcc agctacatcg atgggatgaa agggcgcggc gcccgcctgg
      841 tgttcgcctc ggaccactcc atctccccga acacccgata cgccagctac cagtgggccc
      901 tggaggtcta ccgcaagcac atggcgtact gagaccgcgc gggaaggccg ggcgggaggc
      961 gtcctacttc tgagaagtga ggaacgcctc gatatcggcc gccgtgatga agtgcagcgc
     1021 gaccgcgatc tcgccgaagt gccgagcatc cccggccgcc ttggcgcgca agacctcatc
     1081 cacctgggct tggctcatct tgccggtcct ggtcatgtag tcgccgatcc gttctggcat
     1141 cgcatccctc tctggagcac cgctcgcaac agtatagtct accccccgcg ggtccggatg
     1201 tcgccgtcga gctcggggtt cagcgccacg ctcgcgatgg gcgggtgcac catccggtgg
     1261 accggtcgtt ccggtcatgg gcgccgttcc cgtgtgcggc cggctgcacc ccccaatcgc
     1321 cgatttcctt aatcccctaa agcactatat attgttaagc atatgaggcc ctacggcctc
     1381 cggatcgatc atgggaaagg cggacaacgt gagcggcgag accgtgcagt tcgatgcggc
     1441 ggcgaccccg caggccttgg agcgggtagc ggcgcggctt cgcgagcgca acattgaggt
     1501 gacgatcgtc tcggatgcgc agcaggctcg cgaggcggtg cttcagcgca tacccgcggg
     1561 cgccgaggtc catgccggca agtcgaagtc cctcgtggac atcggggtta cgtcggtgct
     1621 ggcggcggag ggcgcctaca cttacctgcg gaacatcttt ctgaagatgg atcgcaacac
     1681 gcagatgcgg gagatccgaa agctcatttc agccccggac ttcatgctgg ggagcgtggc
     1741 cgcgatcacg gaagatgggg tactggtggc cgcctccgcg accgccagcc agctcggacc
     1801 ctacgcctct ggcgctggga agctgatcct ggttgtcgga agtcagaagg ttgtgccgga
     1861 tctcgaagcc gcgctgcgtc gcatccgtga gtacgtgctg ccgtgggagg acgcccaggt
     1921 gcggcccatg gtgccctccg gttcgtttgt cgggaagatc ctgctgatcg agagggaatg
     1981 ggttgccggg aggatcgagg tgattctggt ggagaagccg gtagggatgt agcaccggtc
     2041 agttgcccag gagaagagtc gaggagtatc aaccgatgaa cgaaaagccg ggacgcaggc
     2101 caaggtgagc cacctcgtct acgcctcaga gtcaggccac gccgccgccg tggatctccg
     2161 ctacctagag agcaagcggc gagtagcgac tcgcccggcc aagccgatgg cg
//
LOCUS       DASRZS010000011         3163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2314955, whole genome shotgun sequence.
ACCESSION   DASRZS010000011 DASRZS010000000
VERSION     DASRZS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2314955"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VFH83_00260"
     CDS             <1..814
                     /locus_tag="VFH83_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET6484816.1"
                     /translation="LKPTIGRVSTRGVLPLSWNLDHVGPLARCVRDAARVLRAIAGYD
                     EADPSSVRMAVGDYLQDLAMDVRGQRIALGVGEHIGIAEREVQEAVGAAARVFESLGC
                     SVREVNVDWMKEAALANRLMTQSDGAAVHRDRLANDPGRFGDDVRRRLEAGAATTSTE
                     YILARRTQAEARRTCDRLFETADLLILPTTPMAAPAIVGYDAVQRAGQLTPFTAPFNL
                     TGLPALSVPCGFTSERLPIGLQIVGKAWEEGRVLRAGHAFEQRTDWHTLGPE"
     gene            complement(903..>3163)
                     /locus_tag="VFH83_00265"
     CDS             complement(903..>3163)
                     /locus_tag="VFH83_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6484817.1"
                     /translation="PLAAVALISIEKTTAALRAATNEQLVTRSTEIARSIDQVFSEEK
                     KIALNLSIDPDIVAAARAVTDTHWAAVPAGATAPAAAAAPAPTPAPATGKAAPGPTAG
                     KTAAKAAPAPDPNAVTSALVSRVTTRLESFMKTKGLGDSYIGIVVTDSDGNTWAASDP
                     IFMGVNAKDRQYWKDAIAGTLNIGAPSLNKVTQKPQTPVAAPIRAGDKVVGVVAMLVD
                     IGFLNDLISGEKIGKTGYAFVVDNTGLDIAHPNADNVFKTNITTVAGMEQASKKMLAG
                     GSGVVEYTFQGVVKTQGYATVPSTGWVVSLTLPQSEYLAPAVQAQNMVLLFGIIAIAV
                     ALVVYFLFSRSITRPLAQGVAFAQTVASGDFTKRLDYNQKDEVGELAQALNGMCLKLK
                     DMVATIQDSAEQVASSSEQISASAQKLSDGSQSQASTLEETSASVEELTASVEQVAEH
                     SQSQTTAVEQGSASMTQVQKAIDEVSGNLVQISALANRSVENAVEGAKAVQQVVSGIN
                     LISQSSEKIGGIITVISDIADQTNLLALNASIEAARAGEHGRGFAVVADEVSKLADRS
                     ASSTKEIEALIKESVKNVTEGVKTALGSQQAMEQIRDASQKVKGMIVSLSDSMNQQVA
                     AIRQLSAALENVSEMSASISAATEEQTTNAKQVSKAVENVNELTQSAASASEEMSAAT
                     QQLSGMAQELQRLMAQFKIVDGALGSSSPRRATVASQKRPALPSSGSPAAGAAERSVD
                     AEALLVGSDSKR"
BASE COUNT          470 a         1027 c         1084 g          582 t
ORIGIN      
        1 cctcaagccg accatcggtc gggtcagcac ccgcggcgtg ttgccgctgt cctggaacct
       61 cgaccacgtg ggcccgctgg cgcgatgcgt ccgggacgcc gctcgggtcc tgcgggcaat
      121 cgccggctac gacgaggcgg acccgtcgtc cgttcgaatg gcggttggcg attacctcca
      181 ggatctggcg atggacgtaa gggggcagcg gatcgcgctc ggggtaggcg agcacatcgg
      241 gatcgcggag cgcgaagtgc aggaagcggt cggcgcggcc gcccgggtct tcgagtccct
      301 ggggtgctcg gtgcgggagg tgaacgtcga ctggatgaag gaggccgcgc tggccaaccg
      361 gctgatgacg cagtccgacg gagcggccgt gcatcgcgat cgcctggcca acgaccccgg
      421 gcggttcggg gatgacgttc gccgtcggct ggaagcggga gccgccacca cctctacgga
      481 gtacatcctg gcccggcgga cgcaggcgga ggcccgaagg acctgcgacc ggctcttcga
      541 gaccgcggac ctgctgatcc ttccaacgac ccccatggcg gcaccggcga ttgtcggcta
      601 tgacgccgtg cagcgcgcgg ggcagctcac gccgttcacg gcgccgttca acctgacggg
      661 tctgcccgcc ctgtccgttc cctgtgggtt cacttcggag cggctcccca tcgggctcca
      721 gatcgtcggc aaggcctggg aggagggccg tgtgcttcga gctggtcatg cattcgagca
      781 gcgaacggac tggcataccc tggggccgga atgagaaaag ggcgcggccg caaggggggg
      841 cttgcgacca cgccagaacg aaaggtgtca gaatccgtgg atcggtcgag atgagcccgc
      901 gctcagcgct tcgaatccga tccgacgagc aaggcctcgg cgtccacgga ccgctccgct
      961 gcgccggccg ccggggatcc gctggatggc agagccggcc tcttctggct ggccaccgtc
     1021 gcccgacgcg ggctcgaaga gcccagggcc ccgtcgacga tcttgaactg cgccatgagg
     1081 cgctgcagct cctgcgccat gccggagagc tgctgggttg cggccgacat ctcttcggac
     1141 gccgaggccg cggactgggt cagctcgttg acgttctcca cggccttgga gacctgcttc
     1201 gcgttggtgg tctgctcctc ggtagcggcg gagatgctgg cgctcatctc actgacgttc
     1261 tcgagcgccg cggagagctg gcggatggcc gcgacctgct ggttcatgga gtccgagagg
     1321 gacacgatca tgcccttcac cttttgggag gcgtcccgga tctgctccat cgcctgctgg
     1381 gaaccgaggg ccgtcttcac gccttcggtg acgttcttca cgctctcctt gatgagggcc
     1441 tcgatctcct tggtggaaga ggcgctgcgg tccgcgagct tgctcacctc gtccgccacc
     1501 acggcgaacc cgcgtccgtg ctcgcccgcc cgcgccgcct cgatggaggc gttgagggcc
     1561 aggaggttgg tctggtcggc gatgtcggag atgaccgtga tgatccctcc gatcttctcg
     1621 gagctctgag agatcaggtt gatgccgctc accacctgct ggaccgcctt ggcgccttcc
     1681 accgcgtttt ccacggaacg gttggccagc gccgagatct ggaccaggtt gccggaaacc
     1741 tcatcgatgg ccttctgcac ctgggtcatg gaggcggagc cctgctccac cgccgtggtc
     1801 tggctctgcg agtgctcggc cacctgctcc acggaggcgg tcagctcctc tacggaggcg
     1861 ctggtctctt ccagcgtgga cgcctggctc tgggatccgt cggagagctt ctgcgcgctc
     1921 gcggagatct gttcgctgga ggacgccacc tgctccgcgc tgtcctggat ggtggccacc
     1981 atgtccttca gcttgaggca catcccgttc agcgcctgcg ccagctctcc cacctcgtcc
     2041 ttctggttgt agtcgagccg cttggtgaag tccccggagg caacggtctg cgcgaaggcc
     2101 acgccttgcg ccagcggccg ggtgatgctg cgggagaaca ggaagtagac gaccagggcc
     2161 accgcgatcg cgatgatccc gaacagcagg accatgttct gggcctggac ggcgggcgcc
     2221 aggtactcag actgcggcag cgtaagggag acaacccagc ccgtagaggg cactgtggcg
     2281 tatccctgcg tcttcaccac gccctggaag gtgtattcga cgactcccga accgccagcg
     2341 agcatcttct tggaggcctg ctccatgccg gcaaccgttg tgatgttcgt cttgaagaca
     2401 ttgtccgcgt tgggatgcgc gatatccagg ccggtattgt cgacgacaaa agcgtatccg
     2461 gttttcccga tcttctcacc cgagatgaga tcgttcagga atccgatgtc caccagcatt
     2521 gcgacgacac ccaccacctt gtcgccggcc cggatggggg ccgcgactgg ggtctggggc
     2581 ttctgcgtca ccttgttcag gctgggcgct ccgatgttca gcgtgccggc gatggcgtct
     2641 ttccagtact gccgatcttt ggcatttacg cccatgaaga ttgggtccga ggctgcccac
     2701 gtattgccat ccgagtcggt aacaacaatc ccgatgtagc tgtccccgag ccctttggtt
     2761 ttcatgaagc tctcgaggcg cgtggtgacc cggctcacca gcgcggaggt aaccgcgttc
     2821 gggtccggcg ccggcgccgc ctttgcggcg gtcttgcccg cagtcgggcc cggggcggcc
     2881 ttgccggtgg caggagctgg cgtcggtgca ggagccgccg cggccgccgg cgcggtagcg
     2941 cctgccggga ccgcagccca gtgagtgtcc gtcacggccc tggccgcggc tacgatgtcc
     3001 ggatcgatgg acaggttcaa ggcgatcttc ttctcctcgg agaacacctg gtcgatggat
     3061 cgggcgatct ccgtggagcg ggtgacgagc tgctcgttcg tagcggccct aagggccgcc
     3121 gtggtcttct cgatcgagat gagcgccact gcggcgagcg gga
//
LOCUS       DASRZS010000012         2820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4239595, whole genome shotgun sequence.
ACCESSION   DASRZS010000012 DASRZS010000000
VERSION     DASRZS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4239595"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VFH83_00270"
     CDS             <1..596
                     /locus_tag="VFH83_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017710771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6484818.1"
                     /translation="TVMPILIAAFGAYAFAWMKFPGRNALFLVVVALLVVPLQMTLIP
                     VLRIFNALHLTGTFLGIWIAHTGYGLPFAIYLLRNFIGDLPAEMMESAALDGASHPTI
                     LFRLVLPTSVPAMASLAIFQFMWVWNDLLVALIYLGGSPPVAPMTVTISNMINSYGGG
                     WQYLTAAAFISMMLPLVVFFSLQQYFVRGILTGSVKG"
     gene            593..2455
                     /locus_tag="VFH83_00275"
     CDS             593..2455
                     /locus_tag="VFH83_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HET6484819.1"
                     /translation="MRSIPRGAAAKAASLTLVCLFAAAQLWADPSGTVDLTILHVNDT
                     HSMLEPSPVRLNVDLGAGLSPATVYVNLGGFPAVMTALARLRSETQGATLFLHAGDFF
                     QGSLYFTEYHGAADVDFWNSMGLDAATLGNHEFDKGPGLIQTALLDKARFPLTSANVD
                     VSREPSITTKDRLAPYVIREVGGQRIGIIGITTPATPYISSPGPNVVFRDPAQSVDAA
                     VQALRAEGVTEIIALTHLGLDADRALASRIHHVALIVGGHSHTLLGSYPSVGLKGIDS
                     YPVVAADADGQTVLIVQAWQWAYQIGRIRVSLDPDGRVVGFDAHPVFVANRGFARIYD
                     LPNLASEKKRVQFTEHAGTLDIREFDGGRYSVPIVESPGDPGDLYHRYEASYRALEAR
                     LTSDPNVFWVDPDPAGQATLDGYAAGVAAFREKPAASVAEEMRRGENSGPGPIIADAM
                     AWQTGAQVAIVNPGGVRTDLVPGELTVARVYELQPFGDTLVTVPVTGEELTRVLEDMA
                     DFTLGSYGTGRRIAHLYVSGLFLSLDASKPKGRRISNVRVREADGSYAALVSTRSYTL
                     VVNSFMAAGGDRNTTLADLPGKYDTGYGDSEALLAYIDGKTLSNVPEERVRRIR"
     gene            complement(2480..>2820)
                     /locus_tag="VFH83_00280"
     CDS             complement(2480..>2820)
                     /locus_tag="VFH83_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6484820.1"
                     /translation="ITHDIASARQFADRIAVMYLGTIVEEGTAAEVAERPAHPYSQAL
                     LAAVPEPDPANRVRLRQVIPGEPPRADAVPSGCPFHPRCPRIIKGRCDVERPAAREIS
                     PGHSVACWLY"
BASE COUNT          425 a          961 c          926 g          508 t
ORIGIN      
        1 ccaccgtgat gcccatcctg atcgccgcct tcggggcgta tgccttcgcg tggatgaagt
       61 ttccgggacg caacgcgctc ttcctggtgg tcgtggctct cctggtggtg cccctgcaga
      121 tgaccctgat ccccgtgctg cgcatattca acgcgctgca cctcaccggc accttcctgg
      181 ggatctggat cgcccatacc ggctatgggc ttccctttgc catctacctg cttcgcaact
      241 tcatcggcga tctgccggcc gagatgatgg agtccgctgc cctggacggg gccagccatc
      301 ccaccatcct cttccggctg gtcctgccca cctcggtgcc cgccatggcg tccctcgcga
      361 tctttcagtt catgtgggtc tggaacgacc tgctggtggc gctgatctac ctgggcggat
      421 ctccgccggt ggcgcccatg acggtgacca tcagcaacat gatcaactcc tacggcggcg
      481 gctggcagta cctgaccgcc gcggccttca tctcgatgat gctcccgctg gtggttttct
      541 tctccctgca gcagtacttc gtgcgcggca tcctgaccgg ctcggtgaaa gggtgaggtc
      601 gatcccaagg ggggccgcgg cgaaggctgc ctctctcacc ctcgtctgtc tgttcgctgc
      661 cgcccagctt tgggcggacc cgtccggcac cgtcgacctg acgatcctcc acgtgaacga
      721 tacccactcc atgctggagc ccagtccggt gcggctgaac gtggatctgg gggccgggct
      781 ctccccggcg acggtctacg tgaacctggg tgggtttccc gcggtcatga cggcgctggc
      841 ccggctccgt tcagagacgc agggcgccac cctcttcctg cacgccggcg acttcttcca
      901 gggcagcctc tacttcaccg aataccacgg cgccgcggat gtggacttct ggaactccat
      961 gggcctggac gcggcaaccc tgggcaacca cgagttcgac aagggaccgg gtctgatcca
     1021 gaccgcgctg ctggacaagg cgcggttccc cctgaccagc gccaacgtcg atgtctcccg
     1081 cgagccgtcc atcaccacga aggatcgatt ggctccgtac gtgatccggg aagtcggtgg
     1141 ccagaggatc gggatcatcg ggatcaccac gccagccacg ccgtacatct ccagccccgg
     1201 tccgaacgtg gtcttccggg atccggcgca gtcggtcgac gccgccgtcc aggcgctgcg
     1261 ggcggaaggc gtcaccgaga tcatcgccct cacgcatctc gggctggacg cggaccgggc
     1321 gctggcttcc cggatccacc acgtggcgct catcgtggga ggccattccc acacgctcct
     1381 gggctcctat ccctccgtcg ggctgaaggg gatcgactcc tacccggtag ttgccgcgga
     1441 cgcggacggc cagacggttc tgatcgtcca ggcctggcag tgggcgtacc agatcgggcg
     1501 catccgcgtc tccctggatc cggacggcag ggtcgtgggc ttcgacgccc atcccgtgtt
     1561 cgtcgcgaac cggggctttg cccgcatcta tgacctgccg aacctcgctt ccgagaagaa
     1621 gcgcgtgcag ttcaccgaac acgcgggcac gctggatatc cgtgagttcg atggcggccg
     1681 ctactcggtg ccgatcgtag agagccccgg agatcccggt gacctctatc atcggtatga
     1741 agcgtcctac cgggccctgg aggcgaggct gacgtcggat ccgaacgttt tctgggtgga
     1801 tccggatccc gcgggccagg caacgctcga cgggtatgcc gccggggtcg ctgcctttcg
     1861 ggagaagccc gccgcgtctg tggcggaaga gatgagacgc ggtgagaact cggggcccgg
     1921 tcccatcatc gccgatgcca tggcgtggca gaccggcgct caggtggcca tcgtgaatcc
     1981 cggcggcgtt cgcacggacc tggtgcctgg agagcttacc gtcgcccggg tctacgagct
     2041 gcagccgttc ggggacacgc tggtgaccgt cccggttacc ggggaagagt tgacccgcgt
     2101 gttggaggac atggcggact tcaccttggg ctcttatgga accggccgca ggattgccca
     2161 cctgtacgtg tcgggcctct tcctttctct ggacgcgtcg aagcccaagg gccgaaggat
     2221 ctcgaacgtg cgggtccgag aggcggacgg ctcctacgct gccctcgtct ccaccaggag
     2281 ctacacgctc gtggtgaaca gcttcatggc ggcgggcggg gaccgcaaca ccacgctggc
     2341 cgatctgcct gggaagtacg acacgggcta cggggacagt gaggccctgc tcgcgtatat
     2401 cgacggcaag acgctctcca acgtccccga ggagcgggtc cggaggatcc gttagctttc
     2461 gctcggcgag ccgggatccc taatacagcc agcaggccac cgagtgcccc ggggagatct
     2521 ctcgcgcggc gggacgctcc acgtcgcagc ggcccttgat gatccgcggg caccgcggat
     2581 ggaacgggca gccggacggg accgcgtccg cgcgcggcgg ctcgccgggg atgacctgac
     2641 gcagacggac gcggttggcg ggatccggct ccggcacggc cgccaggagg gcctgggagt
     2701 aggggtgcgc cggccgctcc gccacctccg ccgcggtccc ctcttccacg atggtgccca
     2761 gatacatgac ggcaatccga tccgcgaact gccgcgcgct cgcgatgtcg tgggtgatgt
//
LOCUS       DASRZS010000013         4846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1544847, whole genome shotgun sequence.
ACCESSION   DASRZS010000013 DASRZS010000000
VERSION     DASRZS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544847"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VFH83_00285"
     CDS             complement(<1..986)
                     /locus_tag="VFH83_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6484821.1"
                     /translation="MRKRQQVETWIRGRIDDGALVPGEKLPSESQLCEQFGVSRNVVR
                     QAIACLSHEGLVESTRGIGTFCKARLPASVSSTNVGLVEFFVNSYVYPEIFRGCYNTL
                     SRRGFAVLVNQSEYNLEQERAILLGLRKNKVGGIIITPIYGAGDRSNAPLLEEMQAEG
                     TAIVLCDDYYPGRSFSSVTLDYQACGEVAATHLWKHGHRKIGVFYQKDFLVKVTRMKG
                     VLDFLGRMEAPVRPPWIVGFNGQGPNGQASITAEHFLKTAKELPTAVVCGNDEDALRL
                     IEAAERRGLEIPRDLSVVGFDNSPIAQMEKVSLTSVDHPSYQIGERAASILL"
     gene            1161..1949
                     /locus_tag="VFH83_00290"
     CDS             1161..1949
                     /locus_tag="VFH83_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6484822.1"
                     /translation="MAETGVRRTALRMVNVEKYFGSVCALKDINLEVGHNEIVGLIGD
                     NGAGKSTLVKILTGVFPPSRGELYIAERKVDTAAYSVKRAHALGIETVYQDRSLGEKQ
                     AMWRNFFIGREMTYPLGFLRVREEKAIANEIMVNTIGFRGRGIGVDSRANKLSGGERQ
                     GVAIGRAMHFEADLIILDEPTVALSLKEVRKVLNFVHKIKEAGKASIYISHNLTDVYE
                     IADRFIVIDRGEIVATMTKDKITLKELDEFLLEYSHGLKDAVQP"
     gene            1946..2995
                     /locus_tag="VFH83_00295"
     CDS             1946..2995
                     /locus_tag="VFH83_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6484823.1"
                     /translation="MSGKRPSFASIGRLEGLPIAAVLAILYFVFILTAPSVFTGHLIY
                     MSFLQTVPPMLVLGIGLTFIITAGEIDLSFPAVVALSGFVFTWWFKNFHTPWIGFLLA
                     LISGGVVGYLNGLLVARLRVPSIMATLAAQFFWYGVTVLIAGGLQIDIASVTTEVIHT
                     IFVGRLFGIVPIQAFWSLGLVVFFWFMLNRHKFGEAIMFIGDNAEVARVMGINVETTR
                     VRLFTMNGVIAAFAGLLLTLEINVFFPTQGQGMLLPVMAAVFIGGTSIAGGQGVLFGT
                     FFGSYIIGSLEAGVVATGIGGYWVQLVEGLVMAAVVVLNVLIGDEGVAFLTKTARKWA
                     VPSSAPADRRGDGKV"
     gene            3130..4110
                     /locus_tag="VFH83_00300"
     CDS             3130..4110
                     /locus_tag="VFH83_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6484824.1"
                     /translation="MRKWILVLLALVFCGILFSAQVWAQEKQIVIYMQCGGTQGDGAT
                     LARTNGARAAAAAAGNVKLIEQYSKWDQQTMIDQFKQAMAAHPDGIEIMGHPGEAAFA
                     ALVDQAEAQGIIVTSGNNPLPTIEAKYQSKGFGYAGADLYAGGFLTGQTMVKAGLKAG
                     DKALVYDIWHQEGRSRSSQGVYDALTKAGLQVEKLDVSQAVDSDTSLAIPILTAYLQA
                     HPDLKAIGTQHGSITAILPKVLQAAGKKPGDIICGGIDLSPATVSAIQGGWISASFDQ
                     VLYLQGYMPVMQIVWTKRYLIPGLHVDTGVGVATPQNIGTLKPLIDQGIR"
     gene            4254..>4846
                     /locus_tag="VFH83_00305"
     CDS             4254..>4846
                     /locus_tag="VFH83_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013794837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET6484825.1"
                     /translation="MKKHVWIAGIALFTFVAAGLFAQTAGLPDLKGRTIRAVTGNDFT
                     PLNFVDPKTGKAVGWEYDAVNEIARRLNAKVAWNVTSWDTMIQAIRDGQFDVGMDGIT
                     ITDERSKQVAFSDPYMTAQQFMLVRKDESRFTGPTDFAANGKLLIGSQAGTTNFYTAV
                     YNVLDGNEQNPRIKLFETFGAAVQALISGDVDCVLLDA"
BASE COUNT          877 a         1475 c         1568 g          926 t
ORIGIN      
        1 tccagtagga tactggcggc gcgctctccg atctggtagc tggggtgatc gaccgacgtc
       61 agggagacct tctccatctg ggcaatcggc gagttgtcga agcccaccac ggagagatcc
      121 cgcgggatct cgaggcctcg acgttccgca gcctcgatga ggcggagcgc gtcttcgtcg
      181 ttgccgcaga ccaccgccgt ggggagctct tttgccgttt tcaggaagtg ctccgcggta
      241 atggatgcct ggccgttggg gccctggccg ttgaacccca cgatccacgg gggccgcacc
      301 ggagcctcca tgcggccaag gaagtccagg actcccttca tgcgcgtgac cttcaccagg
      361 aagtcctttt gatagaagac gccgatcttc cgatggccat gcttccacag gtgggtggcg
      421 gccacctcac cgcatgcctg gtagtcgagg gtcacgctgc tgaagcttcg acccgggtag
      481 tagtcgtcgc agaggacgat ggcggtgcct tccgcctgca tctcctcgag cagcggggca
      541 tttgagcggt ctcctgctcc gtagatcggg gtgatgatga tcccaccgac cttgtttttc
      601 cgcagcccaa gaaggatggc gcgctcttgc tcgaggttgt actcggactg gttgaccagc
      661 accgcgaaac ccctgcgcga aagcgtgttg tagcacccac ggaagatctc gggatagacg
      721 taggagttca cgaagaactc cacgagtccg acgttggtcg acgagacgga agcaggcagg
      781 cgcgccttgc agaaggtgcc gatcccccgc gtgctttcca caaggccctc gtgcgacaga
      841 caggcgatgg cctggcgcac cacattcctc gacaccccga actgctcgca tagctggctt
      901 tcggagggaa gcttctctcc cggaacgaga gccccgtcgt cgatgcggcc gcggatccat
      961 gtctctacct gctggcgttt cctcatggca tgggtcgaat ggtacaagct gtatcttctg
     1021 tagcttcccg tgtcaaggtg gcgattggtc gttagtgtct ctttcgacct aaaaaaaagg
     1081 cttgcagtgc cgccgggcgt atgttaggat gcgcggttga tggaatagct tgtacctttc
     1141 ggaggaagag gagatcctta gtggctgaga cgggtgtcag gcgcaccgcg ttgcgcatgg
     1201 tcaatgtcga gaaatacttc ggttccgtct gcgccttgaa ggacatcaac ctcgaggtcg
     1261 gccacaacga gattgtaggg ctgatcgggg acaacggggc cggcaagtcg acgctggtga
     1321 aaatcctcac cggcgtcttt cccccgtccc ggggggagct gtacatcgcg gaacgcaagg
     1381 tggacaccgc cgcctactcc gtgaaacggg cgcacgcgtt ggggatcgag acggtgtacc
     1441 aggaccggtc tctcggcgag aagcaggcca tgtggaggaa cttcttcatc ggccgcgaga
     1501 tgacctatcc cctggggttc ctgagggtcc gggaagagaa ggcgatcgcc aacgagatca
     1561 tggtcaatac catcggattt cgggggcggg ggattggcgt ggactcccgg gccaacaagc
     1621 tctccggagg ggagcggcag ggagtggcca ttggaagggc catgcacttc gaggcggacc
     1681 tgatcatcct ggacgagccc accgtcgcac tgtccctgaa ggaagtgcgc aaggtgctga
     1741 acttcgtgca caagatcaaa gaggcaggta aggcgagcat ctatatctcc cacaatctca
     1801 ccgacgtcta cgagatcgcc gaccggttca tcgtcatcga ccgcggggag atcgtggcca
     1861 ccatgaccaa ggacaagatc accctgaagg agctggacga gttcctcctg gagtactccc
     1921 acggcctgaa agacgcggtg cagccatgag cgggaagaga ccgtccttcg cttccatcgg
     1981 tcggttggag gggctgccca ttgccgcggt cctggccatc ctgtatttcg tgttcatcct
     2041 cacggcgccg tccgtcttca cgggccacct catctacatg tcgttccttc agaccgtgcc
     2101 gcccatgctc gtgctgggga tcgggctcac gttcatcatc acggccgggg agatcgatct
     2161 gagcttcccg gccgtagtgg cgctatccgg cttcgtgttc acctggtggt tcaaaaactt
     2221 ccatacgcca tggatcggtt tcctcctggc cctcatctcc gggggcgtgg tggggtatct
     2281 caacggtctc ctggtggcgc gcctgcgcgt gccgtccatc atggccaccc tggccgcgca
     2341 gttcttctgg tacggtgtga ccgtcctgat cgccggaggc ctccagatcg acattgcctc
     2401 cgtgacgacg gaggtcatcc atacgatctt cgttggccgc ctcttcggga tcgtgccgat
     2461 ccaggccttc tggtccctgg gcctcgtggt cttcttctgg ttcatgctca accggcacaa
     2521 gttcggcgag gccatcatgt tcatcgggga caacgccgag gtggctcggg tgatggggat
     2581 caacgtcgag accacccggg tgcgtctctt cacgatgaac ggggtcattg ccgccttcgc
     2641 cggcctgttg cttacgctgg agatcaacgt gttcttcccc acccagggcc agggaatgct
     2701 gctgcccgta atggccgccg tcttcattgg ggggacgtcg atcgcgggcg ggcagggcgt
     2761 gctcttcggc acgttcttcg gctcgtacat catcggctcc ctggaggccg gggtcgtggc
     2821 aacgggaatc ggcggctact gggtccagct cgtggaggga ctggtcatgg cggcggtggt
     2881 cgtgctcaac gtccttatcg gggacgaggg ggtcgcgttt ctcacgaaga cggcgcgcaa
     2941 gtgggccgtg ccctccagcg ctcctgcgga tcgtcggggg gatgggaagg tctgatgcat
     3001 cccgcgcgcc gttggcgtgc ggatcgttga acgccattca gaaagggggt gaaccgggct
     3061 tcctctggga acaacggcct tctgcgcctg gcgcagggcc tcaacctatc tccgtaaagg
     3121 gaggacctcg tgcgaaagtg gattttggtt cttctggctc tggtgttctg cgggatcctt
     3181 ttctccgcgc aggtgtgggc gcaggagaaa cagatcgtca tctacatgca gtgcggcggt
     3241 acgcaggggg acggcgccac gctggcacgg accaacgggg ctcgggccgc ggccgccgcg
     3301 gcgggaaacg tgaagctgat cgagcagtac tccaagtggg atcagcagac catgatcgac
     3361 cagttcaagc aggccatggc cgctcacccg gacgggatcg agatcatggg ccatccggga
     3421 gaggcggcgt tcgcggcgct ggtggaccag gcggaagccc agggcatcat cgtcacgagc
     3481 ggcaacaacc ctctccccac gatcgaggcc aagtaccaga gcaaggggtt cgggtacgcc
     3541 ggtgccgacc tctacgcggg tggcttcctg accggacaga cgatggtcaa ggcgggactc
     3601 aaggccggtg acaaggccct ggtgtacgat atctggcatc aggaaggccg cagccggagc
     3661 agccagggcg tctacgacgc gctgaccaag gctggactcc aggtggagaa gctcgacgtt
     3721 tcgcaggcgg tggacagcga cacctccctg gcaatcccca ttctcacggc ctacctgcag
     3781 gcgcatcccg acctgaaggc catcggcacc cagcatggca gcatcacggc catcctgccg
     3841 aaggtgctcc aggcggccgg caagaagccg ggggacatca tctgcggcgg catcgacctg
     3901 tcgcccgcaa cggtgtccgc catccagggc ggctggatca gcgccagctt cgaccaggtg
     3961 ctctatctcc agggctacat gccggtcatg cagatcgtgt ggacgaagcg ctatctgatt
     4021 ccggggctgc atgtcgacac gggcgtcggc gtcgcgactc cgcagaacat cgggaccctc
     4081 aagccgttga tcgaccaggg catccggtag ctccaccctg tcaatccggg cgatctccgc
     4141 ctccggcgga gatcgccatt tcctcacgat gtcggtgagc ggccgcctcc cagacgacgg
     4201 cctccttgtt cgacgggacc gattccttta gtcttggtcg aaaggaggct tccatgaaga
     4261 agcacgtatg gatcgcaggg atcgcgctct tcacgttcgt cgccgcgggg ctcttcgccc
     4321 agacggcggg tcttccggac ctgaagggca ggacgatccg ggcggtcacc ggcaacgatt
     4381 tcacgcccct gaacttcgtg gaccccaaga ccggcaaggc cgtggggtgg gaatacgacg
     4441 cggtgaacga gattgcccga cggctgaatg caaaggtggc gtggaacgtc acctcgtggg
     4501 ataccatgat ccaggcgatc cgcgacggac aattcgacgt ggggatggac ggcatcacga
     4561 ttacggacga gcgcagcaag caggttgcct tctcagaccc ctacatgacc gcgcagcagt
     4621 tcatgctggt gcgcaaggac gagagccgct tcacgggtcc gaccgacttc gccgccaacg
     4681 gcaagctcct cattggctcc caggccggca ccaccaactt ctataccgcg gtgtacaacg
     4741 tcctggacgg caacgagcag aacccgcgga tcaagctctt cgagaccttt ggcgccgcgg
     4801 tccaggctct catctccggg gacgtggact gcgtcctgct ggatgc
//
LOCUS       DASRZS010000014         3073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5010919, whole genome shotgun sequence.
ACCESSION   DASRZS010000014 DASRZS010000000
VERSION     DASRZS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5010919"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VFH83_00310"
     CDS             <1..828
                     /locus_tag="VFH83_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HET6484826.1"
                     /translation="LLSLGSEEDITAVVSLRNFEDERFVLMATRQGVVKKVPISDFAN
                     ARTRGIVAIKLDQGDHLVTALLTTGKDEVVLVTRKGNALRFNEDAVRAMGRASHGVRG
                     IRLSNKDELAGVAMVTSGEQMLLVSEFGFGKRTEYDNFSPHGRGTGGQICYRATEKTG
                     EVIGVLSVSDKDDLVCITSQGNTVKLRLSEIATLGKTAMGVRIVNITKPDVVVGVARV
                     VKDDQPAEGQPDGQSSIGDGTGQPEEPEDSIDASEDESSDDEDASVEPGDEEEDSEE"
     gene            1281..2792
                     /locus_tag="VFH83_00315"
     CDS             1281..2792
                     /locus_tag="VFH83_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HET6484827.1"
                     /translation="MLALYLVSALLLILLNAFFVLAEFASVKVRPTQVEALAESGNRR
                     AKTLEKIQAHLDEYLSVCQVGITLASIGLGFVGEPAFASLLLPVVTSLGLHGLAAGVT
                     AHSVSVAVAYLLVSFLHIVLGELVPKSVAIRSTEKAALRMALPLVVFRYVFFVPNLLL
                     NSTVNGILKLFRLPPVTDHGPHSEEEIRIILDQSQTSGMLSFRRLLHIENVLDMGELK
                     VRNAMRARRLVKTLSLGNTRAQNDNVVSEFKYSRYPLLGDDPEKPLGYVHVKDLFLAE
                     RAGKPTDDLRQFVRPCLSFREQDALENRLSEMQRKAAHMALVYAEDGRWTGIITLEDA
                     VEEVIGTIEEEFALEPTVRLSDLLNIDHTILDVDGTSVLSATRNALQRVKDLPVSLDA
                     IMLSITDRERLGSSYVGRRLAIPHARLARLAQPMVIVARLRAPIPSPVIGEDINLLFI
                     LLTPSDTPRIHQILLSHIAGIFESDFLEDRLEDATTPAELHSVIVTAEQVVLG"
     gene            complement(2853..>3073)
                     /locus_tag="VFH83_00320"
     CDS             complement(2853..>3073)
                     /locus_tag="VFH83_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484828.1"
                     /translation="EVRTTFAPAVLSLEDLRRMAELVRGCARFVIQAFHGGKTLDSAL
                     TDAASPTRSQMNEAASQFQAAGIPVQIR"
BASE COUNT          549 a         1013 c         1002 g          509 t
ORIGIN      
        1 ctgttgtccc tgggcagtga ggaagacatc acggcggtgg tgagccttcg caatttcgag
       61 gacgagaggt tcgtcttgat ggctacccgc cagggcgtgg tgaagaaagt gccgatctcc
      121 gatttcgcca acgcaaggac ccgcggcatc gtggccatca agctggacca gggagaccac
      181 ctggtcaccg cgctcctgac caccggcaag gacgaggtgg tgcttgtgac gcgcaagggg
      241 aatgcgctgc ggttcaacga ggatgccgtc cgtgccatgg gccgcgcgtc ccacggcgtt
      301 cgggggatcc ggttgtcgaa caaggacgag ctcgccggcg tggccatggt cacctcgggg
      361 gagcagatgc tcttggtgag cgagttcggg ttcggcaagc gcaccgagta cgacaacttc
      421 tcgccccatg gccgcggcac cggcggccag atctgctacc gggcaaccga gaagaccggg
      481 gaggtcatcg gggtgctgtc cgtcagcgac aaggacgacc tggtgtgcat cacctcgcag
      541 gggaacacgg tgaagctccg cctcagcgag atcgccaccc tggggaagac ggccatggga
      601 gtccgtattg tgaacatcac caagccggac gtcgtggtcg gagtggcccg ggtggtgaag
      661 gacgatcagc ctgccgaggg acagcccgac ggccagtcca gcatcgggga cgggacgggg
      721 cagccagagg agccggagga ctctatcgac gcgtccgaag acgaaagctc agacgacgaa
      781 gatgcctccg tcgagcccgg ggacgaagag gaagactcgg aggagtaggc gctgtcggcc
      841 actcacggga gactgaacgt ctcctgatgc catagcgctg gatcatgctc cccatccgag
      901 ctcggacgtg ggaatcaacg gcccggacgg gacgcctcag gaatggcacc cgccgaatcg
      961 caacgccttc ccgcgcaccg cccgccggag tcagtgcctc acctcgtggt ggcctggggc
     1021 atcgggccgg ccccgacccc ggaaaacgcg tgacaggcca ctggtttcac gttaaacatc
     1081 cccttgaggg tgccccgccc gcggtgacgc aatgtttcac ggggaacatt tcctctggat
     1141 caaggggccc cggaaggata cgacggctgg agactccatg cttgaatgcc tcagaggcgc
     1201 ttcaagcacg acggcgtccc attcacaacg gcgatccggc agtctaatct gctgctacag
     1261 gaggttccgt aaccaacccg atgctggcgc tctaccttgt gtccgccctg ctgctgatcc
     1321 tgctcaatgc cttcttcgtg ctggccgagt tcgcctcggt gaaggtccgc cctacccagg
     1381 tggaggccct ggcggaatcc ggcaaccggc gggccaagac cctggagaag atccaggctc
     1441 acctggacga gtacctctcg gtctgccagg tggggatcac cctggcgagc atcgggctgg
     1501 gattcgtggg ggagcccgcg tttgccagcc tgcttcttcc cgtggtcacg agcctcggcc
     1561 tccacggcct ggcagcgggg gtcaccgccc acagcgtctc cgtcgccgtg gcgtacctcc
     1621 tggtctcctt tctccacatc gtgctcgggg agctcgtccc caagtccgtc gccatccgca
     1681 gcaccgagaa ggccgcgctg cgcatggccc tcccgctggt ggttttccgc tacgtgttct
     1741 ttgtgcccaa cctcctgctc aactccacgg tcaacgggat cctcaaactc ttccgcctgc
     1801 cccccgtcac ggatcacggg ccccactccg aggaggagat ccgtatcatc ctggaccagt
     1861 cccagaccag cggaatgctg tcgttccgca ggctcctgca catcgagaac gtcctcgaca
     1921 tgggagagct caaggtccga aacgccatgc gggcgcggcg cctggtgaag accctctccc
     1981 tgggaaacac gcgggcgcag aacgacaacg tggtgtccga gttcaagtac tcccgctacc
     2041 ccctgctggg agacgatccc gagaaaccgc tgggctacgt ccacgtgaag gacctgtttc
     2101 tcgccgagcg ggcgggaaag ccgacggacg accttcggca gttcgtccgg ccgtgtctct
     2161 ccttcaggga gcaggacgcg ctggagaacc gcctgtccga aatgcagcgg aaggccgctc
     2221 acatggccct ggtgtacgcc gaggatggcc gctggaccgg gatcatcacg ctggaggacg
     2281 cggtggaaga ggtcatcggg accatcgagg aggagttcgc tctggagccg accgtgcgcc
     2341 tctccgacct gctcaacatc gatcacacga tcctggacgt ggacgggacc agcgtgctgt
     2401 ccgctacccg caatgcgctg cagcgcgtga aggacctgcc ggtctccctt gacgcgatca
     2461 tgctgtcgat taccgatcgc gagcgccttg gaagcagcta cgtcgggcgc cgcctggcca
     2521 tccctcacgc ccggcttgcg cggctggccc agcccatggt catcgtggcc cgccttcgcg
     2581 cgccgatccc ctcgccggtg atcggcgagg acatcaacct cctcttcatc ctgctcaccc
     2641 cgtcggacac gccccgcatc caccagatcc tgctgagcca catcgcgggc atcttcgaga
     2701 gcgacttcct ggaagatcgc ctggaggacg ccacaacgcc ggcggagctg cacagcgtga
     2761 tcgtcacggc ggagcaggta gtgctcggct gatcgcagag accgggggag gggatggatg
     2821 ggggcgatgc cggcacgggc cgcccgtaat ccctatcgga tctgtacagg gatcccggcc
     2881 gcctggaact gtgaagctgc ctcgttcatc tgcgaccgcg tcggtgaggc cgcgtcggtg
     2941 agcgccgagt ccagggtctt ccctccatgg aacgcctgga tcacgaagcg cgcgcagcca
     3001 cgcacgagct cggccatgcg gcgaaggtcc tcgagggaga gtaccgccgg ggcgaacgtg
     3061 gtccggacct cgt
//
LOCUS       DASRZS010000015         7772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4625648, whole genome shotgun sequence.
ACCESSION   DASRZS010000015 DASRZS010000000
VERSION     DASRZS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4625648"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFH83_00325"
     CDS             complement(<1..244)
                     /locus_tag="VFH83_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6484829.1"
                     /translation="MDYVRLGTTGLKVSRICLGTMTYGASKWRDWVLDEAEARPFYKR
                     ALELGITFFDTADMYSQGASEEVTGRALREFARRDEV"
     gene            complement(341..2710)
                     /locus_tag="VFH83_00330"
     CDS             complement(341..2710)
                     /locus_tag="VFH83_00330"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6484830.1"
                     /translation="MKKFFYRLVEWTQHHAWIVVGCVAVITVVMAWLALHVKPNPDMS
                     TLTPEDPAVTRLTEKYGAANKGEDYLIIVARGPALFQPETLARFSAALDQLSALPHVH
                     PGITPFNFPMFETRDGRLGFGMAAEGGVPPSTVGEADQLRQRLLANPQALNLVISPDG
                     SSLAAYLPADSSDDDRPLLKRAEAVIAPLTPVVDIRVSGMVPFSRAILDHIYGDLPIF
                     LGVALVIILLSYYLSFRTKRSLFLPVLIVVLGTIWTVGTMTLLGFKLTIVMVMTPPLV
                     LILGSSYSLHMLNQYYRDTRGLGRGNKDWIVKSVTRIAITIFLAAATTVLGFLSLLTA
                     SLRQVREFGVATAIGITYCALLALFFLPAVLSKLPPPTPVQTGRVREGPLARMLVRLA
                     TWVTRRRWVIVAASLLTVAAFALALTNIRYQTDFMKYFRYREPAVENYQILLDSFTGF
                     ETVYLTLNAPAGATNYFQDPSVLATVARLEDRIRSDPDVKYMLSFNGFLRNMNQAISG
                     SPDIPRSRVPILLLSRYVAALSATQAGKAIAGNLVSPDYTRYTIQFHVWDHVQHSLAF
                     EDRMAQILPRIDSMVRESLPPGVTGEFWGGTMTILSTSAILTRNQVSSIVSSALLVFL
                     IATLIFRSVRHGLVVLIPLGVGLMLDFIFMGIFSIRLDVVTITFASIAIGIGVDNAIH
                     LVVWYRRLRLVHPTDPLATIQQTMKIAGRPMILTTVSIAAALLVFLFSAFKPVLYFGI
                     LIAMSLVLTTAASLIVLPVFLYFGAVRGQRPSGRSVRQRPKRRAAQAAP"
     gene            complement(2902..3588)
                     /locus_tag="VFH83_00335"
     CDS             complement(2902..3588)
                     /locus_tag="VFH83_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET6484831.1"
                     /translation="MITLRYHGHSCFEIDDGTHRVLIDPFLSGNPNATAKPESFTKLD
                     AILITHGHGDHLGDTAAIAKKSGAMVVSNYEIVGYFGSQGVKGHPLHIGGGRQFPFGH
                     VKLTIAHHGSTGPNGEALGSPAGIVLTMGGKKIYHAGDTALFLDMQLIGEAWGPLDAA
                     LLPIGDNFTMSLDDAVRATGFLKAKINIPMHYDTFDLIKADPTEFVRKVEAAGRKAAV
                     VKPGATYTIS"
     gene            complement(3693..5489)
                     /gene="uvrC"
                     /locus_tag="VFH83_00340"
     CDS             complement(3693..5489)
                     /gene="uvrC"
                     /locus_tag="VFH83_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET6484832.1"
                     /translation="MTVTLDPRNRDYPDRPGVYIMKDAQGQTIYIGKARSLKHRVRSY
                     FAGEKDIKTRFLVNDLADIEVIITNTEYEALILESNLIKQRKPRYNIDLKDGKTYPVI
                     RLTAEEYPRVFRTRHMVFDGSEYFGPFPKPQQIDLYLRLIERYFPLCKYRGSRRSRAY
                     PCLNYHMGRCSGACANKITREDYLRIVDGVRKLVSGKTEELLRELRERMAAAAGSLEY
                     EQAAKIRDQIAAIEEISVEQRVMEFVEEDRDYIALAPSGEALVIVVLQVREGKLLGKE
                     VYHVAEYAPDEEAMTHFVSRYYAERKAVPGAVYVDRSVDAEILSTYLRELSGRPIAVK
                     VPQRGKHTQLLAMAAENAREEAAKEALGDRMSAGLDGLKLDLGLAAAPRRIEGFDIAH
                     LDGKDTVASLVRFTDGRADRKLYRAFTIRSLGGRIDDFESIREAVARRYTRVLNEELE
                     RPDLILIDGGKGQLSAALGVLHTLGLDSIPVIGLAKKKEEIFLPGREDSLVLDEASPS
                     LRILEAVRNEAHRFATTLNKKKRAKRVALTVLEGVPGIGQARSRRLMTAFGSVEAMMG
                     ASLEELQKKAGLSRDLAAMLLARLMEKYGKPS"
     gene            5570..7111
                     /locus_tag="VFH83_00345"
     CDS             5570..7111
                     /locus_tag="VFH83_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HET6484833.1"
                     /translation="MTRNRAIGQLHAPAPLVLCILLIACPALSALEKTIELGKDYRWS
                     AIQSTDGVTTAPGRWGDQDLVLTPGAYQPEPDTDLLLHFDAPRTSDETGGYQEVGASP
                     VLSSSISVMGSSAQFGLRGSPVRFQGQAGTLFSPGETWGDFTIEFWLYPATLSDGETV
                     MSWSGSAHPVSESNPTQSLVSQVFRAELRDRRLVWVFQGLFTLPGGDPLPVTLQGTKQ
                     LLPRVWHHHLLRFQARRGTLEYLLDGLPEAIVHTTDSGSETGSIAVPTVGTARSGPLV
                     LGEGLTGYVDELRISRRFVEDPMVARYRDTTGIVVSRVLDLGFSSTRILRIEAVAAAP
                     ADASVEYYYQVADTWDGRPLLGADTSWIAFAPPGDFKSPVQGRFIQLRVELYPDGTRT
                     QSPRVSDLTVVYQPNLPPAFPAGLKASPGNGSVTLSWQPVNDIDVKGYSVYYGTAPHN
                     YLGTGAAEGDSPLDAGTATSLKITGLSNGRLYYFSVVAYDTSQPPQRSDFAAEVSARP
                     SRIYP"
     gene            7117..>7772
                     /locus_tag="VFH83_00350"
     CDS             7117..>7772
                     /locus_tag="VFH83_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6484834.1"
                     /translation="MTRREILVRARNAFKMGEYPLVEKLATRILEESPEDAIAYNLMG
                     SVCERTGRFRRAIELFQKAVDLKPDYTEAHNNLGVLLKRVEAYGEAIPHFERAIELSP
                     RRGDLYYNLGNVYKALGNTEQAEESFQQAIAVDPGFVPSYINLGTVLEAAGRNWDAVR
                     TYQKGLQVDANQPRLRFNLGVVFERTGKLKEARAEYEEAVRALPTWGDAKNNLAVTLH
                     R"
BASE COUNT         1388 a         2478 c         2568 g         1338 t
ORIGIN      
        1 ccacctcgtc ccgacgggcg aactctctga gcgcgcggcc cgtgacctcc tcgctggctc
       61 cctgggagta catgtccgcc gtatcgaaga aggtgatccc cagctccagg gcgcgcttgt
      121 agaagggtcg cgcctcggcc tcgtccagga cccagtccct ccatttcgac gcgccgtagg
      181 tcatcgtccc caagcagatc cgcgagactt tgaggccggt cgtgcccaat cgaacgtagt
      241 ccattcctca cctccgggtg gtcggcgctg ccgtccctct ctaacgtacg gcggcaggat
      301 cgggagagga aagcggaggc tcagaccgtc cggcggaggc tcagggcgcc gcctgagctg
      361 cccgacgctt cggcctctgt ctgacggacc ggccggaggg ccgctgtccc ctcaccgcgc
      421 cgaagtagag aaagacgggc agcacgatca gcgaggccgc cgtcgtcagc accagggaca
      481 tcgctatgag gattccgaag tagagaaccg gcttgaacgc ggaaaagaga aagaccagga
      541 gcgcggcggc gatggatacc gtcgtgagga tcatcggccg acccgcaatc ttcatggtct
      601 gctggatggt ggccagggga tccgtgggat gcacgagccg cagccgacgg taccagacca
      661 ccagatggat ggcgttgtcc acgccgatcc cgatggcgat gctcgcaaag gtgatcgtca
      721 ccacatcgag ccggatcgag aagatcccca tgaagatgaa gtccagcatg aggcccacgc
      781 ccagagggat gaggaccacc aggccgtggc gcacggagcg gaagatcagc gtcgcgatca
      841 ggaacaccag gagagccgat gatacgatgg agctcacctg gttgcgggtg aggatggcgg
      901 aggtagaaag gatggtcatc gtcccgcccc agaactcgcc cgtcaccccc gggggaaggg
      961 actcgcggac catggagtcg atccgcggga ggatctgcgc catgcggtcc tcgaatgcca
     1021 ggctgtgttg gacgtggtcc cagacgtgga actggatcgt gtagcgcgtg tagtcggggc
     1081 tcaccaggtt gccggcgatc gcctttccgg cttgggtggc ggatagcgcg gcaacgtaac
     1141 gggagagcag caggatgggt acccgagacc gcgggatatc cgggctgccg gagatcgcct
     1201 ggttcatgtt tcgaagaaag ccgttgaagg agagcatgta cttcacgtcc ggatccgaac
     1261 ggattcggtc ctccagtcgg gcaacggtgg ccagcacgga cggatcctgg aagtagttgg
     1321 tggcgcccgc cggcgcgttc agcgtgaggt agaccgtctc gaagccggtg aacgaatcca
     1381 ggaggatctg gtagttctcc acggccggct cgcggtagcg gaagtacttc atgaagtccg
     1441 tctggtagcg gatgttggtg agcgccaggg cgaatgctgc gaccgtcaag agggacgcgg
     1501 ccacaatgac ccatcgccgt cgggtgaccc aggtggcgag ccgcacgagc atccgagcga
     1561 gcggcccctc ccggacccgg ccggtctgaa cgggcgtggg gggtgggagc ttggagagaa
     1621 cggccggcag gaaaaacagg gccagcagcg cgcagtacgt gatcccgatc gcggtcgcca
     1681 cgccgaactc ccgtacctgc cggaggctgg cggtgagaag gctgaggaag ccgagcaccg
     1741 tggtggcagc ggcgaggaag atcgtgatgg caatccgtgt caccgacttc acgatccaat
     1801 ccttgttgcc ccggcccagc cctcgcgtgt cccggtagta ctggttgagc atatgcaggc
     1861 tgtaggagct gccaaggatc aggaccagcg gcggggtcat gaccatgacg atggtcagct
     1921 tgaaacccag gagggtcatg gtcccgaccg tccagatcgt gcccaacacg acaatcaaga
     1981 ccgggaggaa cagcgaccgt ttggtacgga agctgaggta gtaggaaagc aggatgatga
     2041 ccagggccac ccccaggaag atgggcaggt cgccatagat gtgatccagg atggcgcggc
     2101 tgaacggcac catgccggag acccggatat cgactaccgg agtgaggggg gcgatcaccg
     2161 cctccgcccg cttcaggagg ggtcggtcgt catcggagga atccgccggc agataggcgg
     2221 ccagcgagct gccgtccggg gagatcacca ggttgagcgc ctgcgggttg gccaggagcc
     2281 tctggcgaag ctggtctgcc tccccgaccg tggaaggagg caccccgcct tccgcggcca
     2341 tgccgaaccc cagacggcca tcgcgggtct cgaacatcgg gaagttgaac ggcgtgatgc
     2401 ccgggtggac gtgcggaagg gcggagagct gatccagcgc ggcggagaat cgcgccaacg
     2461 tctcgggctg gaagagcgcc ggaccgcggg caacgatgat gaggtagtcc tcacccttgt
     2521 tcgccgcgcc gtacttctcc gtaagccggg taacggcggg atcctccggc gtcagggtgc
     2581 tcatgtccgg attgggcttc acgtggagcg ccagccaggc catcaccacc gtgatgaccg
     2641 ccacacaccc cacgacaatc caggcgtgat gctgggtcca ttccacgaga cggtagaaga
     2701 actttttcac ggcagggagc ctcgcgtatg agccaagtgt ggcggagtgc accgccctgg
     2761 gtgcggagtt gaagcgcgtg ctgccgtgga gcgaaaggaa ccctgtccga cggcggcttc
     2821 tggccgccca accaaggacg gatcttagcc gcgcggccgg atcgtgtcaa cgagaaaccg
     2881 ggaacgctac ccgccgttcg ctcaggagat ggtgtaggtg gctcccggct tcacgactgc
     2941 cgccttgcgc cccgcggcct cgacctttcg gacgaactcg gtcgggtcgg ccttgatcag
     3001 gtcgaaggtg tcgtagtgca tcgggatgtt gatcttggcc ttcaggaacc cggtggcgcg
     3061 gaccgcgtca tccaggctca tcgtgaagtt gtccccgatg ggcagcagtg ccgcgtccag
     3121 gggtccccac gcctctccga tgagctgcat gtccaggaaa agcgcggtgt ctcccgcgtg
     3181 atagatcttc ttcccgccca tggtaagaac aatgcccgcg ggactcccga gggcctcgcc
     3241 gttgggcccg gtggaaccgt ggtgagcgat ggtcagcttc acgtggccga agggaaactg
     3301 gcgcccgccg ccgatgtgaa ggggatggcc cttcacgccc tggctgccga agtagccgac
     3361 gatctcgtag ttcgagacga ccatcgcgcc ggacttcttc gcgatggctg ccgtgtctcc
     3421 caggtggtcc ccgtgtccgt gggtgatcag gattgcgtcc agcttggtga agctttccgg
     3481 cttggcagtg gcgttaggat tcccgctcag gaacggatcg atcaggaccc ggtgggtgcc
     3541 atcatcgatt tcgaaacagg agtgtccatg ataacgaaga gtgatcatgg gtcgaaagta
     3601 ctgaaacggg ccgcccgagg caccgactcg gcgcccccca gctgggcggc cccggctggg
     3661 cgcccccggc tgggcgcccc cggctggcct cgtcaggagg gcttgccgta cttctccatg
     3721 agccgcgcca gaagcatggc ggcgaggtcc cgggagaggc ccgccttctt ctgcagctct
     3781 tccaggctgg cgcccatcat tgcctccacg gagccgaagg cggtcatcag gcgccggctc
     3841 cgcgcctggc cgatccccgg caccccttcc agtacggtga gcgcgacccg cttggctcgc
     3901 tttttcttgt tcaaggtcgt ggcgaagcgg tgcgcctcgt tacgaaccgc ttccaggatc
     3961 cggaggcttg gggaggcttc gtccaatacc agggaatctt ctcgacccgg caggaagatc
     4021 tcttcttttt tcttggccag cccgatgacg gggatggaat ccagtccgag ggtgtggagc
     4081 acgcccaggg ccgcggagag ctgcccctta cctccgtcga tcaggatcag gtccggacgc
     4141 tccagctcct cgttgagcac ccgcgtatag cgccgggcca ccgcttcgcg gatggactcg
     4201 aagtcgtcga tcctcccacc gaggctacga atggtgaacg cgcggtaaag cttccggtcg
     4261 gcgcggccgt cggtgaaccg taccagggag gcaaccgtat ccttgccgtc caggtgggcg
     4321 atatcgaatc cctcgatgcg ccgcggcgcc gcggcaagac cgaggtccag cttgaggcca
     4381 tccaggccgg cgctcatgcg atccccgagc gcctccttgg ccgcctcctc cctggcgttc
     4441 tctgccgcca tggccaggag ctgcgtgtgc tttccgcgtt gagggacctt gaccgcgatg
     4501 ggccgaccgg agagctcccg caggtacgtg gacaggatct ccgcgtccac ggatcggtcc
     4561 acgtacaccg ccccgggcac ggccttgcgt tccgcgtagt agcggctcac gaaatgcgtc
     4621 atagcctcct cgtccggcgc gtactccgcg acgtggtaga cctctttgcc caggagcttg
     4681 ccctcccgga cctggagcac cacgatcacc agggcctctc cgctgggagc cagggcgatg
     4741 tagtcccggt cctcctccac gaactccatg acccgctgct ccacggagat ctcttcgatg
     4801 gcggcgatct ggtcccggat cttcgcggcc tgctcgtact ccagggaccc ggccgcggcg
     4861 gccatccgct cccggagctc gcggaggagc tcctccgtct tgccggagac cagcttgcgc
     4921 acgccgtcca cgatcctgag atagtcctcc cgggtgatct tgttcgcgca cgctcctgag
     4981 cacctcccca tgtggtagtt gagacagggg taggctcgac tgcgccggga gccgcggtac
     5041 ttgcagaggg gaaagtagcg ctcgatcaag cgcagataga gatcgatctg ctggggcttc
     5101 gggaaaggac cgaagtactc tgacccatcg aacaccatgt gacgggtccg gaatacccga
     5161 gggtactcct ccgcggtgag gcggatcacc gggtaggtct tcccgtcttt gagatcgata
     5221 ttgtaccgag gcttgcgctg cttgatgaga ttgctttcca ggatcagcgc ctcgtactcg
     5281 gtgttcgtga tgatgacctc gatgtccgcg aggtcgttca ccaggaaccg cgtcttgatg
     5341 tccttttctc cggcaaagta cgaacgcacc cgatgcttga ggcttcgggc ctttccaatg
     5401 taaatggtct gaccctgggc gtccttcatg atgtagacgc cgggtcggtc ggggtagtcg
     5461 cggttcctcg ggtccagggt gaccgtcacg gggcctccct cgatggaacg cgagccgctt
     5521 tcattggaag tctccttatc atccgaatat actactaatg taaacttcga tgactcggaa
     5581 ccgtgcgatt ggacagctcc atgctccggc accactggtc ctctgcatcc tgctcatagc
     5641 gtgccccgcc ctgagcgcgt tggagaagac catcgagctt ggcaaagact accgttggtc
     5701 ggcgatccag agcacggatg gggtcacaac ggctccgggc cgctggggcg accaggacct
     5761 ggtactgacc cccggcgcct atcagccgga gcctgatacc gatctcctcc tccatttcga
     5821 cgcgccgagg acctccgacg agaccggcgg ctatcaggag gtgggcgcct cgccggtcct
     5881 gtcctcaagt atctccgtca tggggtcgag cgcccagttc ggcctccgag gctccccggt
     5941 tcggttccag ggacaggcgg gaaccctgtt cagccccggg gagacgtggg gcgacttcac
     6001 gatcgagttc tggctgtacc ccgccaccct ctcggatggt gagaccgtga tgtcatggag
     6061 cggatcggcg cacccggtct ccgaatccaa tcccacccag tccttggtca gccaggtctt
     6121 tcgggcggag ctccgagacc ggcgcctggt atgggttttc cagggcctct tcacgctgcc
     6181 cggcggggat cctcttccag tgaccctcca ggggacgaag cagctcctcc cccgcgtctg
     6241 gcaccatcac ctcctccgct tccaggcgcg acgggggacc ctggagtacc tcctggacgg
     6301 gcttcccgag gcgatcgtgc ataccacgga cagcggaagt gagaccggct ctatcgcggt
     6361 gcccacggtg ggaaccgcgc gaagcggccc cctggtactg ggtgagggtc tgacggggta
     6421 cgtggatgag cttcgcatct cccgtcgctt cgtggaggat cccatggtgg cccgctaccg
     6481 ggacaccacg ggcatcgtgg tatcccgggt gctcgatctc ggcttctcat cgacccgcat
     6541 cctccgcatc gaagcggtag ccgccgctcc cgccgacgcg agcgtcgagt actactacca
     6601 ggtcgccgac acctgggacg ggagacccct cctgggcgcg gatacgagct ggatcgcgtt
     6661 cgcgcccccg ggggacttca agagcccggt ccagggccgg ttcatccagc ttcgggtgga
     6721 gctgtacccg gacggtactc gcacgcagtc cccacgcgtg tcggatctca cggtcgtgta
     6781 tcagccgaac ctgccgcccg cgttcccggc cggcctgaag gcgtctcccg gcaacggctc
     6841 ggtgaccttg agctggcaac cggtgaacga catcgacgtc aaggggtaca gcgtctacta
     6901 cggcaccgct ccgcacaact acctggggac gggcgccgcg gagggcgact cccccctgga
     6961 cgccggcacc gctacgagcc tgaagatcac cggactttcg aacggcagac tctactactt
     7021 ctcggtggtg gcctacgaca cctctcaacc tccgcagcga agcgacttcg cggcggaggt
     7081 gagcgcccgc ccgtcgagga tctacccgtg aagacgatga cccgtcgcga gatcctggtg
     7141 cgggctcgca acgcgttcaa gatgggcgag taccccctgg tggagaagct ggccacccgg
     7201 atcctcgagg agagcccgga ggacgccatc gcctacaacc tcatgggcag cgtctgcgag
     7261 aggacgggtc ggttccgccg ggccatcgag cttttccaga aggcggtgga cctgaagccg
     7321 gactacacgg aggcccacaa caacctggga gtcctcctca agcgcgtcga ggcgtacggc
     7381 gaggcaatcc cacacttcga gcgggccatt gagctttccc ccaggcgcgg cgacctgtac
     7441 tacaacctgg ggaacgtcta caaagcgctg ggcaacacgg agcaggccga ggagagcttt
     7501 cagcaggcga ttgccgtgga cccgggcttt gtcccctcct acatcaacct gggcacggtg
     7561 ctggaagctg cgggcagaaa ctgggatgcg gtgcggacct accagaaggg tctccaggtg
     7621 gacgccaatc aaccccgcct gcgattcaac ctcggggtgg tcttcgagcg cacgggcaag
     7681 ctcaaggagg ccagggcgga gtacgaagag gccgtccggg ccctgcccac gtggggagac
     7741 gccaagaaca accttgccgt gaccctgcat cg
//
LOCUS       DASRZS010000016         3892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_393117, whole genome shotgun sequence.
ACCESSION   DASRZS010000016 DASRZS010000000
VERSION     DASRZS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393117"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFH83_00355"
     CDS             <1..354
                     /locus_tag="VFH83_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014370468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6484835.1"
                     /translation="LSGVNPELYEAATIDGATKFQKILHIDIPAIVPTVTILLVLRTG
                     AVMSVGFEKAFLMQTPLNLDHSEIISTYVYKVGLQGAQFSFGSAVGLFNSVVNLALLL
                     AVNRVVRALGETSLW"
     gene            365..1243
                     /locus_tag="VFH83_00360"
     CDS             365..1243
                     /locus_tag="VFH83_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010277972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6484836.1"
                     /translation="MRRAREDVIFDTLNYLMVTVFLLLILYPLYFILIASISNPDYVN
                     RGAITFLPRGITFEGYARTFRESNVWRGYRNTLLYTTVGTSINVFLTMTAGYALSRKE
                     LAGRSVIMRLIVFTMLFSGGLIPTYLIVKGLGMTNTLWAMVIPNAVSAWNLIIARTYL
                     QQSIPQELLEASLVDGCSYTRFFTAVVVPLSGAIIAVLALLYSVAHWNSFFTGLIYLQ
                     DYAKYPLQLILRDILLSYSASGAADDAKTLQEMQRVADLMKYALILVASLPVMVLYPF
                     LQRYFVKGVLVGAIKG"
     gene            1255..2394
                     /locus_tag="VFH83_00365"
     CDS             1255..2394
                     /locus_tag="VFH83_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014695705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6484837.1"
                     /translation="MAAETPLPVALIGTGARSSYLYGPLVNALPQAVQLVSVWGRSES
                     SARTLGTQLGVPWFTDVSRLVRETSPRIGIVCVSGQANGQVGLLAVKSGLNVLLETPI
                     AYKLEEADAIIEAAHAHGLSIEVAEQFHRRPKEQIKLRLIARGLLGKVYSSFSDFAGH
                     GYHGVSVMRSYLGFDARPRRVTAVSARFALAPHWSRLAETRGSRVESQDHALVEFEGG
                     QIGVFHWTDVGYDSPLRWWRSSRFLAEKGMGISVGVGIQTQEWISLLAPDGEAPHFIT
                     MERRLERVDGGALVSIVAHTGDPATPTVTWENPFRPLIQGHGVQWSDDEIGVAGCLMS
                     LVDAVRRGTDVTYGPYQARLDQELCIAMQRSAANGSQPVELPIRP"
     gene            complement(2422..3429)
                     /locus_tag="VFH83_00370"
     CDS             complement(2422..3429)
                     /locus_tag="VFH83_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6484838.1"
                     /translation="MAMTRKARWGVLGTARITRTRMVPAVQHSTHGELRGIASRDAER
                     ARRIARELGIPKSYGSYDELLADPEIDIVYNALPNHLHVPWSIRTLRAGKHVLCEKPL
                     ALTATEVRDLIRARDDAGLKAAEAFMVDTHPQWLKARDLTRGAEFGRLRAITGFFSYE
                     NVDPTNNRNILDHGGGAIYDVGSYLIHGARYIWGEEPKRVVSTLERDPVMKTDRLSAL
                     LMEFPSGFASFGCGTQLVRYQRMQFLGTKGRVEIEIPFNAPDDVACRVFFDPGDPGGA
                     GITTHSMPICDQYAIQVDVFSRAVLEDGPVAVPLEDSLENTRIVEAVFESARTGAWVT
                     P"
     gene            complement(3443..>3892)
                     /locus_tag="VFH83_00375"
     CDS             complement(3443..>3892)
                     /locus_tag="VFH83_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6484839.1"
                     /translation="VLARLHLINTKGGLVLVYLIIAMPITVWMLRGFVDTMPVEIEEA
                     ARVDGCGPYGILFRIVVPVLAPAIAAIAMFAFILSWNEFLFPLLITKDLASRTISVGL
                     AFFIDESGVKWGPLMAASVLMGLPPIVAFSYAQKYVVRGLSEGAVKG"
BASE COUNT          655 a         1293 c         1258 g          686 t
ORIGIN      
        1 ctttcgggag tgaaccccga gctgtacgag gccgccacga tcgatggggc caccaagttc
       61 cagaagatcc tccatatcga catccccgcg atcgtgccca cggtgaccat cctgctggtg
      121 ctgcgaaccg gtgccgtcat gtcggtcggt ttcgagaagg cgttcctgat gcagacgccc
      181 ctcaatctcg accattcgga gatcatctcc acctacgtct acaaggtggg gcttcagggg
      241 gcgcaattca gcttcgggtc ggccgtcggc ctcttcaact ccgtggtgaa cctggcgctg
      301 ctgctcgccg tgaaccgggt ggttcgcgcg ctcggcgaga cgagcctctg gtaggggacg
      361 gcgcatgagg cgagcccggg aagacgtgat cttcgatacc ctgaactacc tgatggtcac
      421 ggtgttcctt ctgctgatcc tctacccgct atacttcatc ctgatcgcgt cgatcagcaa
      481 cccggattac gtgaacaggg gggccataac gtttctgccg cgcgggatca ccttcgaagg
      541 ctacgcccgg accttccggg agtcgaacgt ctggcgcggc taccgcaaca cactcctgta
      601 cacgaccgtg ggcacctcca tcaacgtgtt cctcacgatg accgccggtt atgccctctc
      661 ccgcaaggag ctggcgggcc ggtccgtgat catgcggctc atcgtgttca ccatgctctt
      721 cagcggcggc ctcatcccga cctacctgat cgtgaagggc ctgggaatga cgaacaccct
      781 gtgggccatg gtcattccga acgcggtctc cgcctggaac ctcatcatcg ccaggaccta
      841 tctgcagcag agcatcccgc aggagttgct ggaggcttcc ctggtggacg gctgctcgta
      901 cacgcgattc ttcacggccg tcgtggttcc cctttcgggg gccatcatcg cggtcctcgc
      961 gctgttgtac tccgtggctc actggaactc cttcttcacg ggcctgatct acctgcagga
     1021 ttacgcgaag tacccgttgc agctcatcct tcgggacatt ctgctgtcct actcggcgtc
     1081 cggcgcggcg gacgacgcga aaacgctcca ggagatgcag cgcgtcgcgg atctcatgaa
     1141 gtacgccctc atcctggtcg cttcccttcc cgtgatggtg ctctacccgt tcctgcagcg
     1201 gtattttgtc aagggcgtcc tggtgggcgc catcaaaggc tgaggaggaa cccgatggcc
     1261 gcagaaaccc ccttgcccgt cgcgctgatc ggtaccggcg cgcggtccag ctacctgtac
     1321 ggacccctgg tgaacgcgct tccccaggct gtccagctcg tgtccgtctg gggccgcagc
     1381 gagtcctccg cgcgcaccct ggggacgcag ctcggggttc cctggttcac ggacgtcagt
     1441 cgcctggtgc gggagacgag cccccggatc gggatcgtgt gcgtaagcgg acaggccaac
     1501 ggccaggtgg gcctgttggc cgtgaagagc ggcctcaacg ttctcctgga gacgcccatc
     1561 gcctacaagc tcgaagaggc ggatgccatc atcgaggcgg cccacgccca cggcctcagc
     1621 atcgaggtgg ccgagcagtt ccatcgacga cccaaggaac agatcaaact gcgtctcatc
     1681 gccagagggc tcctggggaa ggtatactcg tcgttcagcg acttcgcggg ccacggctat
     1741 cacggcgtca gcgtgatgcg cagctacctg gggttcgacg cccggccgcg gcgcgtcacc
     1801 gccgtttccg cccgcttcgc cctggcgccc cactggtccc ggctggcgga gacgcgcggc
     1861 tctcgggtgg aaagccagga ccacgccctg gtggagttcg agggcggcca gatcggcgtg
     1921 ttccattgga ccgacgtggg atacgactcg cccctgcgct ggtggcggag cagccggttc
     1981 ctggcggaga agggtatggg gatcagcgtg ggggtgggca tccagaccca ggaatggatc
     2041 agcctgctcg cgccggacgg cgaggctccc cacttcatca cgatggagcg gaggctggag
     2101 cgggtcgatg gcggcgcgct cgtgtccatc gtagcgcaca cgggggaccc ggcaacgccg
     2161 accgtgacgt gggagaatcc gttccgtccc ctcatccagg gacacggggt gcagtggtct
     2221 gacgatgaga tcggcgtggc gggttgtctc atgagtctgg tcgacgccgt gcgccgaggg
     2281 actgacgtca cgtacgggcc gtatcaggca aggctggatc aggagctgtg catcgcgatg
     2341 cagcgctccg cggccaacgg aagccagccg gtggagctgc ccatccgacc ctgatctgcc
     2401 gagcgcgtcc gccgcgaccg gctagggcgt cacccaggcc ccggtgcgcg ccgactcgaa
     2461 caccgcctcg acgatgcgag tgttctccag gctgtcttcc aggggcaccg ccaccgggcc
     2521 gtcctccagg accgcgcggg agaagacgtc cacctggatc gcgtactgat cgcagatcgg
     2581 catggagtgc gtggtgatgc cggcgccgcc ggggtcaccc gggtcgaaga acaccctgca
     2641 cgcaacgtcg tccggggcgt tgaagggaat ctcgatctcc accctgccct tcgtgcccag
     2701 gaactgcatc cgctggtagc gaaccagttg cgtgccgcag ccaaagctgg cgaagccgga
     2761 cgggaactcc atgaggagcg ccgagagccg gtccgttttc atcacgggat cccgctcgag
     2821 ggtcgagacc acgcgtttcg gctcctctcc ccagatgtac cgggctccat ggatcaggta
     2881 gctccccacg tcgtagatag cgccgccccc gtggtccagg atgttgcggt tgttggtcgg
     2941 gtcgacgttt tcgtagctga agaagccggt gatcgcccga agccggccga actccgcgcc
     3001 gcgcgtgagg tcccgggcct tcagccactg gggatgggtg tccaccatga acgcctccgc
     3061 ggccttgagg ccggcgtcat cccgcgcgcg gatcaggtcg cgaacctcgg tggcggtcag
     3121 cgccagcggc ttctcgcaca acacgtgctt gccggcgcgg agagtccgga tggaccacgg
     3181 gacgtggagg tggttcggga gggcgttgta aacgatatcg atctccgggt ccgccagaag
     3241 ctcatcgtag gagccgtagg acttcgggat gcccagctcc cgcgcgatcc gtcgcgccct
     3301 ctcggcgtcc cgagaggcga tgccccgaag ctcgccgtgc gtggagtgct gcaccgcggg
     3361 taccatccgg gtccgcgtga tccgggccgt ccccaggacg ccccatcgcg cctttcgcgt
     3421 catcgccatg gatcctcccg ggctacccct tcaccgcccc ttcgctcaac ccgcgaacca
     3481 catacttctg cgcgtaggag aaggccacga taggaggcag gcccatcagc acggaggccg
     3541 ccatcagcgg gccccacttc actccggact catcgatgaa gaaggcgagg ccgacggata
     3601 tcgtgcggct cgccaggtcc ttggtgatca ggagcgggaa gagaaactcg ttccaagaga
     3661 ggatgaaggc gaacatggcg atggcggcga tggcgggcgc cagaaccgga accacgatcc
     3721 gaaacaggat gccgtacggg ccgcatccgt ccacccgcgc cgcctcctcg atctccaccg
     3781 gcatcgtgtc gacaaagccc cggagcatcc acaccgtgat cggcatggct atgatcaggt
     3841 agaccagcac cagaccgccc ttggtgttga tgaggtgcag ccgcgccagc ac
//
LOCUS       DASRZS010000017         5916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5012014, whole genome shotgun sequence.
ACCESSION   DASRZS010000017 DASRZS010000000
VERSION     DASRZS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5012014"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1165
                     /locus_tag="VFH83_00380"
     CDS             104..1165
                     /locus_tag="VFH83_00380"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HET6484840.1"
                     /translation="MNNRMRSCAVGVAAFLALSTLAVVPAAADTASADASIPGVSVST
                     TATAGAFLGVAHEYIYDQNLATNYMVSELVWPLEPMAFAGATVSADTDIGLFGSLDVE
                     QGFAGPAGTMTDSDFLNGDGVRTHYSQSTSYAERANLVELRLGWDLLSRSVLRVGAFG
                     ALSYMDLKWSARDGYYQYPTSGSSYTVSSGTVTPGTYAPWSASETETPLYGTGILYET
                     TYLAAAAGVRARYALGDRFLLDASLAFAPIVDCYVLDNHVLRQLDFYSTLTRGFLVEP
                     RVAVDWALLPSVRVKLDVGYRCAWNLRGNVTEVSTGASDFSTGFPYVAGPNSSFTGTN
                     DSGADLSALDAGLSLSISM"
     gene            1177..2103
                     /locus_tag="VFH83_00385"
     CDS             1177..2103
                     /locus_tag="VFH83_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484841.1"
                     /translation="MSSMLRALAVIGACGILDITLCGCPVQPTYPATITPIYAATGSG
                     SLLVYNGSSWTAHAAGVGSLTSVAVSGSGSGAVAFVGGSGGVAELNGATWTSISGTGA
                     VNALLIGSSLYAATSSGVSVLNADGTTWTSDPTAGPSNAIFTIGPFTYVASNDGLWEF
                     NGTGVVGSPGPTVPLAHVVAGSAKVTAVIVDSALDVFAGTDQGLAIGTGSPLSFGTNQ
                     LPPNTPVNGLAFDSNGNYYAATAAGLYINGTIAVGLSGPVLCVCVDGAGTIYVGTPTG
                     LQVSADGGRTWTVKLSGYQVNAVAVTAPLYSF"
     gene            2223..2633
                     /locus_tag="VFH83_00390"
     CDS             2223..2633
                     /locus_tag="VFH83_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6484842.1"
                     /translation="MESNGKTILVVDDSIQNLKLFKAILSRGGFAVLTVENGDEAITA
                     ARRATPALVLMDLTLPGTDGLEVTRRFKADPDLASIPVVAVTAWARTEDRERALEAGC
                     ADFLAKPVSSKDLVSVARRLTGVTGSDTPPHAGP"
     gene            complement(2596..3579)
                     /locus_tag="VFH83_00395"
     CDS             complement(2596..3579)
                     /locus_tag="VFH83_00395"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HET6484843.1"
                     /translation="MVQIAEILPPRPDPLWRLARQCGIDTVVGTMDFSRGLAVPREDL
                     PWSYTSLVRLKTAYEDNGFRFAVLESRPPMEKIKLGLPGRDQEIDQVRDLLRNLGALG
                     VPVWCYEWMPVFNWVRTSTTVPARGGAMATGYDHSLMAKAPLTEHGVVTEERLWTNLE
                     YFLRAVLPVAEEAGVQMAMHPDDPPLSPIRGLGRIMRSVENFRRLLELRPSPANGVAL
                     CQGNFTLMTDDLPAVIRELGVRGKLFFAHFRDVRGTVEHFVETFHDEGKTNMLACMEA
                     YRDAGYDGVLRPDHVPTMEGDRNDAPGYSSIGRLFAIGYIKGLREAVYRSR"
     gene            3647..4987
                     /locus_tag="VFH83_00400"
     CDS             3647..4987
                     /locus_tag="VFH83_00400"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET6484844.1"
                     /translation="MPLPSFDAPVSAARLDALRQLAEQYRTGGRPALTGEVNNHVHTI
                     YSFSPYSPAAAAERAVSAGLAAVGVMDHDSIAGADEMREAGRILGIATTAGVELRVSA
                     AGTPLEGRKINNPDSVGILYMMIHGVPRRSLPAVRRFLRPIQASRHERDQRMVRALNA
                     LLPGFDLPALDWERDVLAQSQASDGGTITERHILFAVARSMVQVTGLGRPLAALLRDR
                     LRIAVPARLEALLTDPANPHLLYDLLGLLKSSFLQQVFLQPDDRECIPVRQAVRFAEE
                     VGAIPCYGYLGDVTDSPTGDKKAERFEDSFLDEMMPVLRELGFRAIAYMPPRNTREQL
                     LRLRRLCSANSFMEISGVDVNSPRQSFNCPELLLPEFAHLVSATWALIAHERLGDAGE
                     RYGLFHSDNPLAGKSLEERIEEYAAVGRSLDPTGERDPATEPLVLQWERGRGRR"
     gene            5066..>5916
                     /locus_tag="VFH83_00405"
     CDS             5066..>5916
                     /locus_tag="VFH83_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6484845.1"
                     /translation="MKKERGNHGARVAITVIVLAFASSLSIRADDQTAPSPSSALAVV
                     TLTQCVEQALSAGPGVRLSNANLASAKAQYTVSAAANSLGLGGSVAVNHSGAPYDTRY
                     YAPGTLITGQDTARAGLSLTAPLSTSVGLSAGHTIDELTPPGQLTDLSLSASSTIWDG
                     YLGGKALAAVKQASLVLQGTQTSEDANQKGIVYSVKQAYYTLLGAQQQLSILEQTLTQ
                     RQQELQKTQALLDAEQANQIDLKQARVNLTQASLDLKKAQDAVEIDRETLSGLVGWIL
                     DKSYAVA"
BASE COUNT          951 a         2034 c         1917 g         1014 t
ORIGIN      
        1 ggctcggcgc ccccggctgg caccggccgg gcgccccccg gctcggcgcc cccggctggc
       61 accggcccgg gaatgcgtct atagtcgagg cgaagatgtg cctatgaaca atcgcatgcg
      121 ctcctgtgcc gtaggtgtgg cagcattcct tgccctgtcg acccttgccg tggtcccggc
      181 cgcggcggac acggcgagcg cggacgcctc gataccgggg gtctccgtca gcacgaccgc
      241 aaccgccggg gccttcctcg gggtcgctca tgagtacatc tacgatcaga acctcgcgac
      301 gaactacatg gtctccgagc tggtctggcc ccttgagccg atggccttcg cgggagctac
      361 cgtgtcagcc gacacggaca tcgggctctt cggctcgctc gacgtcgagc agggattcgc
      421 gggtccggcg ggaaccatga cggactccga cttcctgaac ggcgacggtg ttcggaccca
      481 ttactcccag agcaccagct acgccgagcg ggccaacctg gtggagctcc gtctgggctg
      541 ggacctgctg agccggagcg tcctgagggt cggcgccttc ggggcgctct cctacatgga
      601 cctcaagtgg tccgctcggg atgggtacta ccagtatccc acctcggggt cgtcctacac
      661 cgtgtcgagc ggaacggtga ccccggggac ctatgctccc tggtcggcct ccgagaccga
      721 gacgccgctg tacggcaccg gcatcctgta cgagacgacc tacctggccg cggccgccgg
      781 ggtgcgcgcc cgatacgccc tgggggaccg gttcctgctc gacgcctctc ttgcgttcgc
      841 tcccatcgtg gactgctacg tgctggacaa tcacgtcctg cggcagctcg acttctactc
      901 gacccttacg cgcggcttcc tggtcgagcc gcgtgttgcc gtggattggg cgttgctgcc
      961 atcggtccgc gtgaagctcg acgtgggcta tcggtgtgcg tggaacctca ggggcaacgt
     1021 gaccgaggtc agcacgggcg ccagcgactt ctcgactggt ttcccgtatg tcgccggccc
     1081 gaactcctcg ttcaccggca ccaacgactc cggagcggac ctctccgcgt tggacgcagg
     1141 cctcagtctc agcatctcga tgtagggggc atggcgatga gcagcatgct acgagccctg
     1201 gcggtcatcg gtgcctgcgg cattctggat atcaccctct gcggctgtcc ggtccagcca
     1261 acctatccag ccacgatcac accgatctac gcggccaccg gctccgggag cctgctggta
     1321 tacaacggct cgtcctggac cgcgcacgcc gcgggtgtgg gatctctcac cagcgtggca
     1381 gtcagcggct ccggctcggg cgcggtggcg ttcgtgggtg gaagcggcgg cgttgccgag
     1441 ctcaacggag ccacctggac ctctatctcc gggacgggcg cggtgaatgc gctcctcatc
     1501 gggtcgagcc tttacgcagc cacttccagc ggcgtgtcgg tcctgaatgc ggacgggacg
     1561 acctggacca gcgaccccac cgccggtccg tccaacgcga tcttcacgat cgggcctttc
     1621 acctacgttg cctcgaatga cgggctctgg gagttcaacg gcacgggcgt ggtcggctct
     1681 ccaggaccca cggttccgct ggctcacgtc gtggcgggct ccgccaaggt caccgcggtc
     1741 atcgtggact ccgccttgga cgtctttgcc gggacggacc agggtttggc gattgggact
     1801 ggcagcccat tgagcttcgg caccaaccag cttcccccga acacaccggt gaacggcctg
     1861 gccttcgact ccaacggcaa ctactacgcc gccaccgctg ccggactcta catcaacggc
     1921 acgatcgccg tgggtctctc gggcccagtt ctctgcgtct gtgtcgacgg cgccggaacg
     1981 atctacgtgg ggactccgac cgggctgcag gtttccgccg atggcggccg cacctggacc
     2041 gtcaagcttt cgggctacca ggtgaacgcc gtggccgtca cggccccgct gtattcgttc
     2101 taggcccgaa cctacggatc ccgtttgact tgcggtcggc ccgggtgtag gattccgaac
     2161 gcgtcgggcc agagttgcgc aacccggttg cgtatgccag tgcgtgcgga ggtgcgcgtc
     2221 ctgtggagag caacgggaag acgatactcg ttgtcgacga cagcattcag aacctgaagc
     2281 tcttcaaggc gatcttgagc cgtggcggct ttgccgtgct cacggtggaa aacggcgacg
     2341 aggcgatcac ggccgccaga cgcgccacgc ccgccctggt cctcatggac ctcacgctgc
     2401 cgggaaccga cggtctggag gtgacccggc gcttcaaggc ggatccggac ctcgcctcca
     2461 tccccgtggt agcggtcacc gcctgggccc gcacggagga ccgtgagcgc gccctggaag
     2521 ccgggtgcgc ggatttcctg gccaagcccg tgagctcgaa agacctcgtc agtgtcgccc
     2581 gccgcctcac cggggtcacc ggctccgata caccgcctca cgcaggccct tgatgtatcc
     2641 aatcgcgaag agccggccga tcgaagagta gccgggcgcg tcgttgcggt ccccttccat
     2701 cgtaggcacg tggtcaggac gaaggacgcc atcgtatccg gcgtcccgat acgcctccat
     2761 acacgccagc atgttcgtct tgccctcatc gtggaaggtc tcgacgaagt gctccacggt
     2821 cccgcgcacg tcgcgaaagt gggcgaagaa gagcttgccc cgaacgccca gctcccggat
     2881 gacggcgggg agatcgtcgg tcatgagggt gaagttcccc tggcaaaggg ccacgccgtt
     2941 ggccggactg ggccgcagct ccaggagccg tcggaagttc tccacggacc gcatgatccg
     3001 tcccagcccc cggatggggg agaggggcgg gtcgtccgga tgcatggcca tctggacgcc
     3061 cgcctcctcg gcaacgggaa gcacggcccg caagaagtat tccaggttgg tccacaagcg
     3121 ctcctcggtg acaacgccgt gctccgtgag gggggccttg gccatgaggg agtggtcgta
     3181 accagtggcc atggcgcccc cgcgagcggg caccgttgtg gaggtccgaa cccagttgaa
     3241 gacgggcatc cactcgtagc accacaccgg gacgccgagg gctccaagat tcctcaggag
     3301 gtcgcggacc tggtcgatct cctggtctct ccccggcagc ccgagcttga tcttctccat
     3361 cggcggccgg ctttccagca ccgcgaagcg gaagccgttg tcttcgtagg cggtcttcag
     3421 ccggaccagg gaggtatagc tccaggggag atcctcccga gggaccgcga ggccccggct
     3481 gaaatccatg gtcccgacta ccgtgtcgat tccgcactgc cgcgccagcc gccacaaggg
     3541 gtcgggtcga ggcggcagga tctccgcaat ctgcaccatc gttcggggag tatgcgagac
     3601 gccgctcgca ttgacaaggc tggcgggccg agggcacact ttcgcgatgc cgctaccttc
     3661 cttcgacgcc cccgtgagcg ccgcgcgtct ggacgccctc cgccagcttg cggagcaata
     3721 ccgaacgggc ggccgcccgg ccctgaccgg cgaggtgaac aaccacgtcc acacgatcta
     3781 ctccttcagc ccgtacagcc cggccgcggc cgcggagcgt gccgtgtccg cggggttggc
     3841 ggcggtgggc gtcatggacc acgactccat tgcgggggcg gacgagatgc gggaggcggg
     3901 ccggatcctg ggtatcgcca cgaccgcggg cgtggagctt cgggtgagcg ctgccgggac
     3961 tcccctggag ggccgcaaga tcaacaaccc ggattcggtc ggaatcctct atatgatgat
     4021 ccatggcgtg ccgcggcgaa gcctgccggc cgtgcgccgg ttcctgcgac ccatccaggc
     4081 ctcccgccac gagcgggacc agcgcatggt ccgggccctg aacgcccttc tgccggggtt
     4141 tgacctgccg gcgctggact gggagcggga cgtccttgcc cagtcccagg cctcggatgg
     4201 cggcaccatt acggaaaggc acatcctctt cgcggtggcc cggtccatgg tccaggtcac
     4261 gggtctcggg cggcctctgg ccgcgctcct ccgggaccgc cttcggatcg ccgtgcccgc
     4321 ccggctggaa gccctcctga ccgacccggc caaccctcac ctcctctacg accttctggg
     4381 tctcctcaag agctccttcc tgcagcaggt cttccttcag ccggacgacc gggagtgcat
     4441 ccccgtccgg caggcggtcc ggttcgcgga ggaggttggc gccatcccat gctacggata
     4501 cctgggagac gtcaccgatt ctcccacggg agacaagaag gcggagcgat tcgaggacag
     4561 ctttctggac gagatgatgc ccgtgctccg tgagctgggg tttcgggcca tcgcctatat
     4621 gccgccccgc aacacccggg agcagctcct gcgtctgcgg cggctgtgct ccgccaactc
     4681 cttcatggaa atcagcggcg tggacgtgaa cagcccacgc cagtccttca actgtccgga
     4741 gcttcttctg ccggagttcg ctcacctggt ttccgccacc tgggccctga tcgcgcacga
     4801 gcgcttgggg gacgcggggg agcgttacgg gttgttccat tccgacaatc cgcttgccgg
     4861 gaagtcgttg gaagagagga tcgaggagta cgctgccgtt ggcaggtccc tcgaccctac
     4921 gggggaacgg gatccggcga ccgagccgct cgtcctccaa tgggaaaggg gccgcgggcg
     4981 cagataacaa aacgttatcg tgatttcacc cgctatctat ggtgttcgac ggcctctccc
     5041 tatagcctct tacacggaga aacggatgaa aaaggaacgt gggaaccacg gggcgagggt
     5101 cgcgatcacg gtcatcgtac tcgcattcgc ctcctccctc tccatccgtg cggacgatca
     5161 gaccgctcca tctccttcct ctgcacttgc tgtcgtgacc ctcacccagt gcgttgagca
     5221 ggccctctcc gccgggccgg gcgtccgcct gtccaacgcg aacctcgcct ccgccaaggc
     5281 ccagtacacc gtttcggcgg cggcgaactc cctcggcctg ggcggcagtg tcgccgtcaa
     5341 ccacagcggc gctccgtacg acacccgcta ttacgccccc ggcaccctca tcacgggcca
     5401 ggatacggcc cgcgcgggtt tgagcctgac cgcgccgctt tccacctcgg tggggctgag
     5461 cgccggccac acgatcgacg agctcacgcc cccgggtcag ctcacggacc tgtccctctc
     5521 cgcgagctcg accatttggg acgggtatct cggcggcaag gcgctggcgg ccgtgaagca
     5581 ggcctcgctg gtcctgcagg gcacccagac ctccgaagac gccaaccaga agggcatcgt
     5641 ctattccgtg aaacaggcct attacacgct tctgggcgcg cagcagcagc tatccattct
     5701 ggagcagacg ctgacccagc gccagcagga gctgcagaag acccaggcac ttctggacgc
     5761 ggagcaggcc aaccagatcg acctgaagca ggcgcgggtg aacctcacgc aggccagcct
     5821 cgatctcaag aaggcgcagg acgcggtgga gatcgaccgc gagacattgt ccggcctggt
     5881 cggctggatc ttggacaaga gctacgcggt cgcgga
//
LOCUS       DASRZS010000018         3610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774 k141_9431,
            whole genome shotgun sequence.
ACCESSION   DASRZS010000018 DASRZS010000000
VERSION     DASRZS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9431"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFH83_00410"
     CDS             <1..188
                     /locus_tag="VFH83_00410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET6484846.1"
                     /translation="AQSGAALAGAPPPMTVEDTTQVLGRHLLGDFVLPFELVSVLLLS
                     ALIAAIAIIAKDREDEA"
     gene            185..505
                     /gene="nuoK"
                     /locus_tag="VFH83_00415"
     CDS             185..505
                     /gene="nuoK"
                     /locus_tag="VFH83_00415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019153946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET6484847.1"
                     /translation="MTITLTHYLALGAILFCLGVYGAISRKNAIGILMSLELVFNAVN
                     LNLVAASRWQPTGPQGSAVGQLFALFVIAAAAAESVLGLALILAIYRRRLVVNADEMD
                     LMKN"
     gene            517..2388
                     /gene="nuoL"
                     /locus_tag="VFH83_00420"
     CDS             517..2388
                     /gene="nuoL"
                     /locus_tag="VFH83_00420"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET6484848.1"
                     /translation="MPDPVTAAILAPLLPLAAFVLALAVTKRRAGVAAGISITAVAVA
                     LLLSLWLLRGQLLSAGPIERELPWLHAGSLQISVGVLIDPLASLMMVIVTAVSLLVQI
                     YSLGYMKGDPAFARFFSYLSLFTFSMLTLVIANNFILLYMAWELVGLCSYLLIGFWYQ
                     RPAAASAAKKAFIVTRFGDFGFLAGILVLSVLAGGFGLGDAQRLVSSGGLAAGQVTLI
                     ALLLFCGAIGKSGQFPLHVWLPDAMEGPTPVSALIHSATMVAAGVFMVARLYGVFQGS
                     ADAMRVVASLGAFTALFAAAIALAQDDIKRVLAYSTISQLGYMMLALGVGGYGAGIFH
                     LTTHAAFKTLLFLCAGSVIHALGTNDIWKMGGLARRMPITAVTFLVAVLAISGIFPLS
                     GFWSKDEILQAALSSGHIELYVVALVTVFLTAFYMARVFFVVFSGPSAPHAHESPAVM
                     TIPMIVLGLLAIGLGAIGLPWLSRDIHTFLAPTAPRPSGLDLAVLLESTTLATAGIIL
                     ALLFYGLRLVKPESLRRAAGPAYTVLARKFYIDELYLLVIRGLFFAVTEAIAWFDRHV
                     VDGAVNLVGAASLRGGGALRKSVSGKVQGYALIVVCGVTVTLVVLLLVNRSLIFGGR"
     gene            2388..>3610
                     /locus_tag="VFH83_00425"
     CDS             2388..>3610
                     /locus_tag="VFH83_00425"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET6484849.1"
                     /translation="MGFPVLSAIVALPLLSVLVLFCLPPGARNAIRGVSLAGAASAFA
                     LTVWLFFAYDRGVGGLQFVERLPWLTTFGVSYYVAVNGVNVMLLLLTGVIITAGVLAS
                     WNVTYRPKEFFILLLTLTTGVFGVFLSYNLFLFFLFYEVSVLPMYLLIGVWGTGRKEY
                     AAMKLTLYLMAGSALILAGILGLYFAAGKQSFDLDVLSRATYDPRFQKVFFPILFAGF
                     ANLAALWPLHTWSPDGHSSAPTAVSMLHAGVLMKLGAYGCLVVPMSLLPDGARTWLPL
                     VAILCIVNIVYGSLGAVAQRDLKYIVAYSSVSHMGIVLLGLASRNETALDGAVLQMFS
                     HGVMTGLFFALVGMIYGRTHTRMVDEQGGLAARTPVLAAFFVLTGLTSLGLPGLSGFP
                     AELSVFLGAFRVYPGV"
BASE COUNT          445 a         1231 c         1174 g          760 t
ORIGIN      
        1 gcgcccagtc cggtgccgcg ttggccggtg cgccgccccc gatgaccgtt gaggacacca
       61 cccaggtcct gggccggcac ctcctgggtg atttcgtcct cccgttcgag ctcgtctcgg
      121 ttctcctctt gtcggccctg atcgcggcaa tagccatcat cgcgaaagac cgggaggacg
      181 aggcgtgacc atcacgctga cccactacct ggcgctgggc gccatcctct tctgcctggg
      241 cgtctacggg gcgatcagcc ggaagaacgc catcggcatc ctcatgtctc tggagctggt
      301 attcaacgcg gtgaacctca acctggtggc cgcgtcgcgc tggcagccca cgggcccgca
      361 ggggagcgcg gtgggacagc tcttcgccct gttcgtgatc gccgcggcag cggcggagag
      421 tgtactgggg cttgccctga tcctggccat ctaccgccga cgcctcgtgg tgaacgcgga
      481 cgagatggac ctgatgaaga actgaaaggg ggggatgtgc ctgatcccgt gaccgccgca
      541 atccttgcgc ccctgctccc gctggcggct ttcgtccttg cgctggcggt gacgaaacgg
      601 cgcgcggggg ttgccgccgg gatctccatc accgcggttg ccgtagcgct cctcctctcc
      661 ctctggctcc tccgaggtca gctcctctcc gcggggccca tcgagcgcga gctcccgtgg
      721 ctccacgcgg ggagcctcca gatcagcgtc ggggtgctga tcgatcccct ggcctccctg
      781 atgatggtga tcgtcacggc ggtgagcctc ctggtgcaga tctactccct gggctacatg
      841 aagggggacc cggcgttcgc ccggttcttc tcctacctgt ccctgttcac gttctccatg
      901 ctcaccctgg tcattgccaa caacttcatc ctgctgtaca tggcctggga gctggtgggg
      961 ctgtgctcgt acctcctcat cgggttctgg taccagcggc ccgccgcggc gtccgccgcc
     1021 aagaaggcgt ttatcgtgac ccgcttcggc gatttcggct tcctggccgg gatcctggtg
     1081 ttgagcgtgc tcgccggggg cttcggcctg ggggacgcgc agcggctcgt cagctccggc
     1141 ggcctggcgg cgggccaggt gacgctgatc gcgctcctgc tcttctgcgg cgccatcggc
     1201 aagagcgggc agttccccct gcacgtctgg ctgccggacg ccatggaggg cccgacgccg
     1261 gtgtccgcgc tgatccactc cgccaccatg gtggcggcag gggtcttcat ggtggcgcgc
     1321 ctctacgggg tcttccaggg gagcgcggac gcgatgcggg tggtcgcctc cctgggcgcg
     1381 ttcaccgcgc tgttcgcggc cgccatcgcg ctggcccagg acgacatcaa gcgcgtgttg
     1441 gcctactcca cgatcagcca gctcggctac atgatgctgg ccctgggggt gggcggctac
     1501 ggcgccggca tcttccacct gaccacccat gcggccttca agacgctcct cttcctctgc
     1561 gccgggagcg tgatccacgc attgggcacg aacgacatct ggaagatggg cggcctggcc
     1621 cgccggatgc cgatcacggc cgttacgttc ctggtcgccg tgctggccat ctcggggatc
     1681 tttccgctgt cggggttctg gagcaaagac gagatcctcc aggcagccct ttccagcggg
     1741 catatcgagc tctacgtagt ggcgctggtc accgtgttcc ttacggcttt ctacatggcg
     1801 cgcgtgttct tcgtggtgtt ctcggggccc agtgcccctc acgcccatga aagccccgcc
     1861 gtgatgacga tcccgatgat cgtgctcggg ctcctggcga tcggtctcgg agccatcggg
     1921 ctgccgtggc tttcgcggga catccacacg ttcctggcgc ccaccgcgcc ccgtccctcg
     1981 gggctggacc tcgccgttct gctggagtcc acgacattgg ccaccgcggg gatcatcctg
     2041 gccttgcttt tctacgggct gcggctggtc aagcctgaga gtctccgccg cgcggcaggt
     2101 ccggcttata cggtccttgc gcgcaagttc tacatcgacg agctgtacct cctcgtgatc
     2161 cgtgggttgt ttttcgcggt cacggaggcc atcgcctggt tcgaccgcca cgtggtggac
     2221 ggcgcggtca atctggtggg cgccgcctcc ctgcgcggcg gcggagcgct gcgcaagagc
     2281 gtctccggca aggtgcaggg atatgccttg atcgtggtct gcggggtgac ggtgaccctg
     2341 gtggtcctcc tcctcgtgaa ccgatctctc atcttcgggg gccgatagtg ggattcccgg
     2401 tgctctccgc catcgtcgcc ttgcctcttc tctcggtcct ggtgctcttc tgccttccgc
     2461 caggcgcccg aaacgccatc cggggcgtct cgctggccgg tgccgcttcc gcgttcgcgc
     2521 tgacggtgtg gctcttcttc gcgtacgacc gcggagtcgg cgggctgcag ttcgtggagc
     2581 gccttccatg gctcaccacc tttggcgtga gctactacgt ggccgtcaac ggggtcaacg
     2641 tgatgctcct cctcctgacg ggcgtgatca tcaccgccgg ggtcctggcg tcgtggaacg
     2701 tgacctatcg gcccaaggag ttcttcatcc tcctcctcac gctgaccacg ggtgtattcg
     2761 gcgtgttcct gtcgtacaac ctgttcctgt tcttcctctt ctacgaggtg tcggtgctgc
     2821 cgatgtacct cctcatcgga gtctggggca ccgggaggaa ggaatacgcg gccatgaagc
     2881 tgacgctgta cttgatggcc ggcagcgctc tcatcctggc cggcatcctg gggctgtact
     2941 tcgccgcggg gaagcagtcc ttcgacctgg acgtgctctc gcgggccacc tacgatcccc
     3001 gcttccagaa ggtgtttttc ccgatcctct tcgccggctt cgccaacctg gcggccctct
     3061 ggccgttgca cacgtggtcg ccggacgggc actcctccgc ccccaccgcg gtctccatgc
     3121 tccacgcggg cgttctgatg aagctgggag cctacggctg cctggtggtg cccatgagcc
     3181 tgctgcccga tggcgcgcgg acctggctgc cgctcgtggc gatcctctgt atcgtcaaca
     3241 tcgtctatgg ctccctgggc gccgtggccc agcgggatct gaagtacatc gtggcatact
     3301 ccagcgtcag ccacatgggg atcgtgctgc tgggccttgc gtcccgaaac gagaccgcgc
     3361 tggacggggc ggtcctgcag atgttctctc acggcgtcat gactgggctc ttcttcgcgc
     3421 tggtgggaat gatctacggc cggacccaca cgcggatggt ggacgagcaa ggcggcctgg
     3481 cggcccggac gccggttctc gccgcgttct tcgtgctcac gggcctcacc tctctggggt
     3541 tgccggggct gagcggtttc ccggcggagc tctcggtctt cctgggcgcc ttccgagtct
     3601 accctggggt
//
LOCUS       DASRZS010000019         9116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4626780, whole genome shotgun sequence.
ACCESSION   DASRZS010000019 DASRZS010000000
VERSION     DASRZS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9116
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4626780"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..729
                     /locus_tag="VFH83_00430"
     CDS             136..729
                     /locus_tag="VFH83_00430"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6484850.1"
                     /translation="MQVRSEETRGRILKAASKLFAEKGFESTGVAEICTESDVSKGAL
                     YHHFPSKQAIFLELLQDWLSGIDRGLTTALDDAPSVPEALLAMSAQMRNVFSAARDQL
                     ELFLEFWQQARRDPQVWKQLIEPYRRYQDYFAKIIQKGIDQGSIRQVDPQAAGQALVA
                     LAVGIVLQGVLDPGGAQWDLVTQNSLRLLLQGLGARE"
     gene            742..2595
                     /locus_tag="VFH83_00435"
     CDS             742..2595
                     /locus_tag="VFH83_00435"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6484851.1"
                     /translation="MVLVTGAAGHVGSALVRELLAEGEKVRALVLPDEELSGLEGLDV
                     EIVFGNVLDRDALRSAMRGIDVVYHLAGIVSILPGRNDLMRTVNVVGTAAVARVARES
                     LVRRMVYVSSIHALARPPRGTPIDESVRFDPHNTAGEYDQTKAEASLAVLAEVERGLD
                     AVLLCPTGIIGPFDFRSTSALANQVRAWMKPGWHVTVNGQFDFVDVRDVGRAMILASR
                     SGKRGETYIVSGERISVGRLFALVRRSAQASGRSNGGAILVPFGLAMLAAELATIHAK
                     LWKCSVQFTRYSLETLVSNSVIDGTKAHRDLGYQPRPMAETVRDTVGWLSQQAAVVPS
                     RRAGTGRARVPDRGPSRSKPPARVAVVTGASSGIGAATATRLGSLGYRVVLVARREDR
                     LQELAAEIRRAGGAADVLALDLSDPNGIRAVYDHVTGLGEGLDVLVNSAGFGWYGYGS
                     DMPVQTAGQMVAVNSGALAQLIVLFLPLMRARGRGCIINVSSIAGSFASPWSALYSAT
                     KSFADTFTRALHRELRGTGVSVCAVRPGPVLTEFYQTVSRLSEGRSIPVERFATRPET
                     VVDAIVSLLRRPRPAVYVPRGFAVLPWLELSLGWLFDRVAALLLRRQGSAA"
     gene            complement(2630..3037)
                     /locus_tag="VFH83_00440"
     CDS             complement(2630..3037)
                     /locus_tag="VFH83_00440"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HET6484852.1"
                     /translation="MKDVLFLIGSESDREAVDPGVKLLEEKGISHDVRVISAHRDLEK
                     LLAFLKAEEGGYRLVIAAAGLSAALPGVVASLVKLPVIGIPLVAGALSGIDALLSILQ
                     LPRGVPVATMGVGKQGVLNAVLLAERMLRMPKE"
     gene            complement(3034..4362)
                     /gene="purD"
                     /locus_tag="VFH83_00445"
     CDS             complement(3034..4362)
                     /gene="purD"
                     /locus_tag="VFH83_00445"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET6484853.1"
                     /translation="MKVLVLGSGAREHAIAWKLAASKDVEQTFVGPGNAGTADIAENL
                     PTVDPLNVASVLEACRSRGITCVFVGPEAPLAAGVVDLLSARRIPAVGPRASAARLES
                     SKAFSKAFLVRNRIPTARAAEFDDREAFEQHVHRHAGERLVVKKSGLAAGKGVLESSD
                     AEALLDFGRGVLSGDHLLVEEFLEGWEVSIFGVSDGTSYAVLPPCTDFKKAGDGDTGP
                     NTGGMGAICPVPWVDARLLKAIRGQIVEPTYAAMDREGLSYAGILYFGLMITRDGPKV
                     LEFNVRLGDPETQVLLPSLDLDFGKLVEAMANGTLAKLRLGDAAARASGAALGVVVAG
                     AGYPESTPRGLAVEPILGLVEKHALAFHASTQRDANGVLRTGGGRCFTVVGRGRDLKA
                     AFQQAYAHVDAVRFQGGWYRKDIGRKFLEERHPPKPAPVGSNGRRAGGMV"
     gene            complement(4359..4952)
                     /gene="purN"
                     /locus_tag="VFH83_00450"
     CDS             complement(4359..4952)
                     /gene="purN"
                     /locus_tag="VFH83_00450"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET6484854.1"
                     /translation="MARLAVFASGRGSNFVAIAQGLRAAGRHRLELLLCDRKGAPAFQ
                     RAAEMHVPALYVSYQGRPRSEAEREILGHLRRAGIQVVALAGFMRLLTPDFLREFPGP
                     VLNLHPSLLPRHPGTHGIEESFRSEDRELGISIIRVDEGVDTGPILLQKAFPRSPGET
                     LAQVEERIHALEHAWFPQVVLEMLDRVDPEPTREEVS"
     gene            complement(4952..5941)
                     /gene="purM"
                     /locus_tag="VFH83_00455"
     CDS             complement(4952..5941)
                     /gene="purM"
                     /locus_tag="VFH83_00455"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET6484855.1"
                     /translation="MDTYRSAGVDIDRGDRFVEFIKSIRSKAVDPGIGGFSAALPIDT
                     LGFRSPVLMTTTDGTGTKLLVARALGKFDTIGIDLVAMNVNDLLVCGARPITFLDYIA
                     CGKLREDRLHEIMRGIVRGCELAGCTLGGGETAEMPDLYDPEDFDLAGFAVGMAEKDG
                     VLPRMGDIREGDRILGLPSSGIHSNGLSLARKVIPQSDRAGWEELLTPTIIYARQMRA
                     LAATGKLLAAAHITGGGLFGNLQRVIPAGLAPRFTFRWDVPPVFDRIQSLGGISTDEM
                     RRVFNLGIGITLIANPQDVAALNEVARRESFPLLDIGELIPASPGRDAGAGAV"
     gene            complement(6039..7436)
                     /gene="purF"
                     /locus_tag="VFH83_00460"
     CDS             complement(6039..7436)
                     /gene="purF"
                     /locus_tag="VFH83_00460"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET6484856.1"
                     /translation="MAGEGLGHHCGVVGISSEVEINLPELLFYGLFSLQHRGQESAGL
                     AYQRGDTIKSYRGVGMVTNALGHYLREDHPSRLGIGHVRYSTHGTNKLENAQPIVVSC
                     SKGEIALAHNGNISNSETLQAGLIAEGSIFQSTTDTELLLHMIARSRRPSFKEALIEC
                     LGAIEGAYCFLLMHGGKLIAVRDPYGFRPLVLGTRDGQVLIASETCALEVFQARNVRE
                     IEPGELVIVEGTRITSERLPSPPPPRKAHCVFELIYFARPDSIIFRQTVHMMRKRMGA
                     KLAMGEDREADIVIAVPDSGNSAALGYSATSGLPLEQGLTRNHYMGRTFIQPTPAMRE
                     FGVRLKLHAVREAIEGKRVIVVDDSLVRGTTSRQIVKLLRDAGAREIHVRFSSPPLKF
                     PCFFGIDIPTREELIANRLDPDGIAREIGADTVRFLPMSALRESVPEPRDYCYACFSG
                     EYPFPVRDAPVAKKN"
     gene            complement(7468..8598)
                     /gene="dusB"
                     /locus_tag="VFH83_00465"
     CDS             complement(7468..8598)
                     /gene="dusB"
                     /locus_tag="VFH83_00465"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET6484857.1"
                     /translation="MRSFGRCPRRSSAKSCSPTVSGSLASPTDGPRAGLDAALLHPVR
                     LGPLTAADNLWLAPMAGYTDAAFRAICARHGATLCFTEMVSAEGLAHDSRPTLRLLER
                     RPEETIYGVQIFCSDPVSAAVSARRIGAYQPAILDLNCGCSVPKILKGGCGAALLRDP
                     RRIGAIVRAMRAETGIPVSVKLRLGWDASSLTYLTCAEEAVSAGAVLVALHPRTRSQG
                     FGGKAEWEHIQRLKRASSVPVFGSGDLLSPDACLSMLRDTGCDGILIARGTLGNPFIF
                     EQTRALLTGRPMVLPDARQRLAVAMDHLRMTAGLVGEPKACRDMRKHFVAYTRGMEGG
                     AALRQALVHAVTLAQYEGIVAEYLGDPSPDRPPSPDGRSSPD"
     gene            complement(8513..>9116)
                     /locus_tag="VFH83_00470"
     CDS             complement(8513..>9116)
                     /locus_tag="VFH83_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET6484858.1"
                     /translation="IYHSIGISPSSVVQPGEDWEAKIEDGTRLERVVAIGEIGLDYYR
                     KFGDRDSQVELFIRQLELAEKLNLPVIIHNRDAGSDVLEILRERLPRRGGILHCYSED
                     YEYARKALDLNLWISFAGNVTYRNARNLQDTAKKVPLERVLIESEAPFMVPALYRGKR
                     NKPSYINETATFVAQLREVPPEELSEVLFANSLRVFGLAD"
BASE COUNT         1491 a         3084 c         3062 g         1479 t
ORIGIN      
        1 cgctgtcatc ccccgtacgg tcatcccctt ctctgctgac ggttcccaag tctgagcagc
       61 ttgacggcgc aagtgtcttg tggtatgttg aaaccgaccg gtcggtctgg ttggtgacgc
      121 gccaaggagg ctcgcgtgca ggttcgaagt gaggagacgc gcggtcggat cctgaaggct
      181 gcctcgaagc tgttcgcgga aaaggggttc gagtccaccg gggtcgcgga gatctgtacg
      241 gagtccgatg tcagcaaggg cgccctgtac caccatttcc cgagcaagca ggcgatcttc
      301 ctggagctgc tccaggattg gctgtccggg atcgatcgcg ggctcacgac ggcgctcgac
      361 gacgccccga gcgttccgga ggctctcctt gccatgagcg cccagatgag gaacgtgttc
      421 agcgctgctc gggaccagct cgagctgttc ctcgagttct ggcaacaggc gcgtcgggac
      481 ccgcaggtct ggaaacagct catcgagccg taccgcaggt accaagacta tttcgcgaag
      541 atcattcaga agggcatcga tcaaggctcc atccggcagg tcgacccgca agccgccggc
      601 caggccctgg tcgcactcgc cgttgggatc gttctgcagg gcgtcctgga tccgggggga
      661 gcgcagtggg atctcgtgac ccagaacagc ctgcggctgc tgctgcaggg ccttggcgcg
      721 cgggaatagg ggaggtaacg aatggttctt gtcaccggtg ccgccgggca cgtcgggagc
      781 gctctcgtgc gagagctcct ggccgaaggg gagaaggttc gagcgttggt cctgccggat
      841 gaggagcttt cggggcttga ggggctggac gtggagatcg tcttcggaaa cgtcctggac
      901 cgggatgccc tgcgctcagc gatgcggggc atcgacgtgg tctaccatct ggcggggatc
      961 gtctccatcc tgccggggag aaacgatctc atgcggacgg tgaacgtggt tggaaccgcc
     1021 gccgtggccc gcgtcgcgcg ggagagcctg gttcgccgga tggtctatgt cagctcgatc
     1081 cacgcgctgg cgcggccgcc ccgaggcacc ccgatcgatg agagcgttcg gttcgaccct
     1141 cacaacaccg ctggtgagta cgaccagacc aaggcggaag cgtccctggc cgtgctggcg
     1201 gaggtggagc gcggactgga cgcggtcctc ctgtgcccga cgggcatcat cgggccgttc
     1261 gacttccgct ccacctccgc cctggccaac caggtgcgcg cgtggatgaa gccgggatgg
     1321 catgtgaccg tcaacggcca gttcgacttc gtggatgtgc gcgacgtggg gcgcgccatg
     1381 atcctggcga gtcggtcggg caagcgcgga gagacctaca tcgtttcggg tgagcgcatc
     1441 tccgtcggtc gcctcttcgc cctggtgcgg cgatccgcgc aggcgtcggg acggagcaac
     1501 gggggcgcta tcctggtacc gttcggcctg gccatgctgg ccgccgagct tgctacgatc
     1561 cacgcgaagc tgtggaagtg ctccgtccag ttcacgcggt attccctgga gacgctcgtc
     1621 agcaactccg tcatcgacgg aacgaaagcg caccgggacc tgggttacca gccaagaccc
     1681 atggcggaga cggttcggga taccgtgggt tggctgagcc agcaggccgc cgtggttccg
     1741 tcgcggcgcg cgggaacagg ccgggcgcgc gttccggacc gcggcccgtc ccgcagcaag
     1801 ccgccggccc gcgtcgccgt ggtcacggga gcctccagcg gtatcggcgc cgccaccgcg
     1861 acccgcctgg ggagcctggg gtatcgcgta gtgctcgttg cccgccggga ggaccgactg
     1921 caggagcttg ccgcggagat ccgccgggcc ggcggcgccg ccgacgtcct tgccctggac
     1981 ctctccgatc cgaacgggat ccgcgccgtc tacgaccacg tgaccggact cggggaaggc
     2041 ctggacgtgc tggtgaacag cgccggcttc ggatggtacg ggtacgggtc ggacatgccc
     2101 gtccagacgg ccggccagat ggtggccgtg aacagcggcg ccctggcgca gctcatcgtc
     2161 cttttcctgc ccctgatgcg agcgcgggga cgcggatgca tcatcaacgt cagctcgatc
     2221 gcgggcagct tcgccagccc gtggtccgcc ctctattcgg ctaccaagtc gttcgctgac
     2281 accttcacgc gggctttgca ccgggagctt cggggaaccg gagtctccgt ctgcgccgtg
     2341 cgcccgggac ccgtgctcac ggagttctac cagacggtga gccgcctctc cgagggccga
     2401 tccatccccg tggaacgctt cgccacccgg cccgagaccg tggtggacgc catcgtgtcc
     2461 ctcctccgca gacctcgacc cgccgtgtat gtgccccggg ggttcgccgt tctgccgtgg
     2521 ctggagctca gcctgggatg gctattcgac cgggtggcag ccctgctgct ccgcaggcag
     2581 ggctccgcgg cctagcggtg cctcgttctg ccgttcgcgg tggtccgggt tactctttgg
     2641 gcatccgaag catccgctcg gcgaggagga ccgcgttgag cacgccctgc ttgccgacgc
     2701 ccatggtcgc cacggggacg ccgcggggaa gctggaggat cgagagcagg gcgtcgatgc
     2761 cggacagcgc gccggcgacc agcgggattc cgatcaccgg aagcttgacc agggaggcca
     2821 ccactcccgg gagcgccgcg gagaggcccg cggccgcgat caccaggcgg tagccgccct
     2881 cctcggcctt caggaaggcc agcagcttct cgaggtcacg atgcgcggag atcaccctca
     2941 cgtcgtggga aatgcccttc tcctccagga gcttgacccc cggatccacc gcttcacggt
     3001 cggattccga gccgataagg aagagcacgt ctttcatacc atgcctcctg cccgacgccc
     3061 gttgctgccg accggagcag gcttgggggg atgccgctct tccaggaact tccgtccaat
     3121 gtccttgcgg taccagcctc cctggaaccg cacggcatcg acatgggcgt aggcctgctg
     3181 aaacgcggcc ttgaggtctc gcccccgtcc cacaaccgtg aagcacctgc cgccccccgt
     3241 gcgaagcacc ccgttggcgt cccgctgggt agaggcgtgg aaggccagcg cgtgtttctc
     3301 caccaggccc aggatcggct ccaccgccag accgcgtggc gtggactccg ggtaaccggc
     3361 gcccgccacc acgacgccga gggccgcgcc gcttgctcgc gcggccgcgt cgcccagccg
     3421 cagcttcgcc agcgtgccgt tggccatcgc ttccacgagc tttccgaagt ccaggtccag
     3481 ggaaggcagg aggacctggg tctccggatc gccgaggcgt acgttgaact cgaggacctt
     3541 cggaccgtcc cgggtgatca tgaggccgaa atacaggatc cccgcgtagg acaggccctc
     3601 acggtccatg gccgcatagg tcggctccac gatctgacct cggatcgcct tgagcagcct
     3661 ggcgtccacc cagggcaccg ggcatatcgc acccattccg cccgtgttgg gtccggtgtc
     3721 cccatcaccg gccttcttga agtccgtgca gggaggcagc accgcgtagc tcgtgccgtc
     3781 gctcaccccg aaaatggaga cctcccagcc ctccaggaac tcctccacca ggaggtggtc
     3841 tccactcagc acgccgcgcc cgaagtccag gagcgcctcc gcgtccgatg actccagcac
     3901 ccccttgccg gccgcgagac cgctcttctt gaccacaagg cgctcgccgg cgtgacgatg
     3961 cacgtgctgc tcgaacgcct cccggtcgtc aaactcggcg gcccgggcgg tgggaatccg
     4021 gtttcgcacc aggaacgcct tggagaacgc cttgctggac tccagccgag ccgccgacgc
     4081 gcgaggtccc accgccggga tccggcgggc ggagaggagg tctacgacgc cggcggccag
     4141 gggcgcctcg gggcccacga agacgcaggt gatgccacgg ctgcggcagg cctccaggac
     4201 ggatgccacg ttgagaggat cgaccgtcgg gaggttctcg gcgatgtccg ccgtaccggc
     4261 gttccctggg ccgacaaacg tctgttcgac gtccttgctc gccgcaagct tccacgcaat
     4321 ggcgtgctcc cgggctcccg acccgagcac cagcactttc atgagacctc ctcccgcgtc
     4381 ggctccggat cgacgcggtc cagcatctcc agcaccacct gggggaacca cgcgtgctcc
     4441 agcgcgtgga tccgctcctc cacctgtgcc agggtctccc cggggcttcg cgggaacgcc
     4501 ttctgcagca ggatgggccc ggtatccacc ccctcgtcca cgcggataat ggagatcccc
     4561 agctcccggt cttcggatcg gaagctctcc tcgatcccgt gcgtgccggg gtgcctgggc
     4621 aggagactgg ggtgcaggtt gaggacgggg ccggggaact cgcgcaagaa gtccggcgtc
     4681 aagagccgca tgaagccggc cagggcaacc acctggatgc cggcgcggcg caggtgaccc
     4741 aggatctcgc gctccgcctc cgagcgcggc cggccctggt aggatacgta gagcgccggg
     4801 acgtgcatct ccgcggcgcg ctggaaggcg ggcgcgccct tgcggtcgca gaggagcaac
     4861 tccaggcgat gacgccccgc cgcgcgcagt ccctgcgcga tggccacgaa gttggacccg
     4921 cggccggagg cgaacacagc cagcctggcc atcagacggc ccccgccccg gcgtccctcc
     4981 caggagaggc ggggatcagc tccccgatgt ccagaagcgg gaagctctcc cgtcgcgcta
     5041 cctcgttcag cgcggccacg tcctgcgggt tcgcgatcaa cgtgatgccg atgcccaggt
     5101 tgaagactct gcgcatctcg tcggtggaga tgcctcccag ggattggatc ctgtcgaaaa
     5161 cgggaggcac gtcccaccgg aaggtgaacc ggggcgcgag gccggcgggg atgacccgct
     5221 ggaggttccc aaagagcccg ccgccggtga tgtgagccgc ggccagcagc ttcccggtcg
     5281 ccgccagggc ccgcatctgg cgggcgtaga tgatggtcgg ggtcaggagc tcctcccaac
     5341 ccgcccgatc cgactgcggg atcaccttgc gcgccagaga gagaccgttg gagtggatcc
     5401 cggaggacgg gagcccgaga atgcgatctc cctcccggat gtcccccatc cgcggaagca
     5461 ccccgtcctt ctcagccatt ccgacggcga agcccgccag atcgaaatcc tcggggtcgt
     5521 acaggtccgg catctccgcg gtctcaccgc cgcccagggt gcagccggcc agctcgcatc
     5581 cgcgcacgat cccacgcatg atctcgtgca ggcgatcctc acggagcttc ccgcaggcga
     5641 tgtagtccag gaaggtgatc ggccgggcgc cgcacaccag aaggtcgttc acgttcatgg
     5701 ccacgaggtc gatcccgatg gtgtcgaact tcccgagcgc cctcgccacg agcagcttgg
     5761 tgccggtgcc gtccgtggtc gtcatgagga cgggggatcg gaagcccagg gtgtcgatgg
     5821 gcaacgccgc ggagaagccg ccgatcccgg ggtcgaccgc cttggatcgg atgctcttga
     5881 tgaactcgac gaaccggtct ccccggtcga tgtccactcc ggccgaacgg tacgtgtcca
     5941 tggaaccccc tgccgctccc gtgctcccat tcgcgatcgg ttcggcgcgc ggcccgaacg
     6001 aatcccgtct gggcccgatg aggcccgcgc gtaggccctc agttcttctt tgctaccggc
     6061 gcatctcgaa ccgggaaggg atactcaccg ctgaagcagg cgtaacagta gtcccgcggc
     6121 tccggcacgg actcgcggag cgccgacatg ggcaggaacc tgaccgtgtc ggccccgatc
     6181 tcgcgggcaa tgccatccgg atccagacgg ttcgctatca gctcttcccg ggtcgggata
     6241 tcgattccaa agaagcacgg gaacttcaga ggcggggagg agaaccgcac gtggatctct
     6301 cgcgcccccg cgtcgcgaag cagcttcacg atctggcgtg aggtggtccc gcggaccagg
     6361 gagtcgtcca ccacgatgac tctcttccct tcgatggctt cccgcacggc gtggagcttg
     6421 agccgcacgc cgaactcccg catggccggc gtgggctgga tgaaggtcct gcccatgtag
     6481 tggttgcggg tgagcccctg ctccagcggg agccccgagg tagcggaata gcccagcgcc
     6541 gcgctgttgc cggagtccgg caccgcgata acgatgtccg cctcccggtc ctcgcccatc
     6601 gccagcttgg cccccatgcg ctttcgcatc atgtgaacgg tctgacggaa gatgatggag
     6661 tccgggcgcg cgaagtagat cagctcgaag acgcagtggg ccttccgcgg gggcggcggc
     6721 gatggcagcc gctccgaggt gatccgggtc ccctccacga tcaccagctc ccccggctcg
     6781 atctctctca cgttcctggc ctggaagact tccagggcgc aggtctccga ggcgatcagc
     6841 acctggccat cccgggtccc caggaccagg ggacgaaagc cataggggtc ccgcaccgcg
     6901 atgagcttgc caccgtgcat gagcaggaag cagtacgccc cctcgatggc ccccagacac
     6961 tcgatcagcg cctccttgaa gctcggccgg cgcgaccggg caatcatgtg cagaagcagc
     7021 tccgtatccg tggtgctctg aaagatggaa ccttccgcga tcagacctgc ctggagcgtc
     7081 tcggagttcg agatgttgcc gttgtgcgcc agggcgatct cgcccttgct gcaggagacc
     7141 acgatgggct gcgcgttctc cagcttgttg gtcccgtggg tggagtatcg cacgtgaccg
     7201 atcccgagcc gggacggatg gtcctctctc aggtaatggc ccagcgcgtt cgtcaccatg
     7261 ccaacgcccc ggtaggactt gatggtgtcc ccgcgctggt acgccagacc ggcgctctcc
     7321 tggccccgat gctggagtga gaaaagcccg tagaagagga gctcgggcag gttgatctcc
     7381 acttcggagg agatccccac cacaccgcag tggtgcccaa gtccttcacc tgccacccgg
     7441 atatgaaact gaaaacggca gggcgagtca atccggactc gagcggccat ccggactcgg
     7501 aggtcgatcc ggactcgggt cgcccagata ctcggccacg atcccctcat actgggcgag
     7561 cgtgacggcg tgcaccaggg cctggcgaag cgcggccccg ccctccatcc cgcgcgtgta
     7621 ggcgacgaag tgcttgcgca tgtcgcggca cgccttgggc tccccgacaa ggccggccgt
     7681 catccgcagg tggtccattg ccacggcgag gcgctggcga gcgtcgggga ggaccatcgg
     7741 ccgtcctgta agaagtgctc gggtctgctc gaaaatgaag ggattcccga gggtcccccg
     7801 cgcgatcagg atcccgtcac accccgtgtc ccgaagcatg ctcaggcagg catccggcga
     7861 gaggaggtcc ccggacccga acacggggac cgaggaggct cgcttcagcc gctggatgtg
     7921 ctcccactcc gcctttccgc cgaagccctg ggagcgggtc cggggatgaa gggctacgag
     7981 cacggcgccc gctgaaactg cctcctcggc gcaggtgagg tacgtgaggg aggacgcatc
     8041 ccaaccgaga cgcaacttga cggaaacggg aatgccggtt tcggcgcgca tggcccgtac
     8101 gatggcgccg atccgccgag ggtcgcggag caaggcagcg ccgcatccgc ccttgaggat
     8161 cttggggact gagcagccgc agttcaggtc cagaattgcc ggctgatagg cgccaatccg
     8221 ccgcgccgag accgccgcgg acacgggatc cgagcagaag atctgcacgc cgtagatggt
     8281 ctcctcgggt cgacgttcca gcagtcgcag ggtcggccgg ctgtcatgcg ccagcccctc
     8341 cgcggagacc atctccgtaa aacacagggt ggcgccgtga cgggcgcaaa tggcgcggaa
     8401 ggcggcgtcc gtgtagccgg ccatcggcgc cagccagagg ttgtcggcgg ctgtcagcgg
     8461 tccaagccgg accggatgca gcagagcggc gtcgagcccc gcccgtggcc cgtcagtcgg
     8521 cgaggccaaa gacccggaga ctgttggcga acaagacttc gctgagctcc tccgggggca
     8581 cctcccgaag ctgcgcaacg aacgtcgccg tctcgttgat gtaggagggc ttgttccgct
     8641 tgcctcggta gagggcaggc accatgaagg gagcctcgct ctcgatgagc acccgctcca
     8701 ggggaacctt cttggcggtg tcctggagat tgcgggcgtt ccgataggtc acgttcccgg
     8761 caaaggagat ccacaggttg agatccagag ccttacgcgc gtattcgtag tcctcggagt
     8821 agcaatggag aatgccccca cggcggggca ggcgctcccg gaggatctcc agcacgtcgg
     8881 agccggcgtc ccggttgtgg atgatcaccg gcaggttgag cttctccgcg agctccagct
     8941 ggcggatgaa caactccacc tgggagtcgc ggtcgccgaa cttccgataa taatcgagac
     9001 cgatttcccc gatggcgacc acgcgctcca ggcgcgtccc gtcctcgatc tttgcctccc
     9061 agtcttctcc cggctggacg acggaggagg gggaaatccc gatggagtgg tagatc
//
LOCUS       DASRZS010000020         8765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_780081, whole genome shotgun sequence.
ACCESSION   DASRZS010000020 DASRZS010000000
VERSION     DASRZS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8765
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780081"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..918
                     /gene="sufC"
                     /locus_tag="VFH83_00475"
     CDS             130..918
                     /gene="sufC"
                     /locus_tag="VFH83_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010196650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET6484859.1"
                     /translation="MTHNLRVKDLKANIGDKPILTGLDLEIRTGEIHALMGPNGYGKS
                     TLANVLMGHPSYTVTGGSVTLDGEDFLALKPSERALRGMFLAFQYPVEIPGVTVGRFI
                     KRALELRDGGKGMNVTAYIKELRQSMDVMGIDQVFINRYVNQGFSGGEKKRMEVLQML
                     MLKPSLALLDETDSGLDIDALKVVAQGVNRMRGDGFGALIITHYQRILTLVVPDFVHI
                     MYKGRIATSGGKELVEQLESKGYDWVRQQFGIEEEAHASASAGR"
     gene            893..2314
                     /gene="sufB"
                     /locus_tag="VFH83_00480"
     CDS             893..2314
                     /gene="sufB"
                     /locus_tag="VFH83_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET6484860.1"
                     /translation="MPAPAQAVDKSPTVDIGEYQHGFSYPDQSVFKTRKGLDEEVVNA
                     ISQHKGEPDWMRQFRLRALSAFRKKSMPNWGGDLSDLDFENLYYYAKPQGEQSRTWEE
                     VPANIKETFDRLGIPQAERKFLAGVGAQYDSEMVYHSINADLAKLGVIFTDPETAVRE
                     HPDLVRKYFGTVIPYLDNKFAALNSAVWSGGSFVYVPKGVKVEIPLQAYFRINARSFG
                     QFERTLIIADEGSFVHYIEGCTAPVYATESLHSAVVEIIALPGSRVRYTTIQNWSNDV
                     YNLVTKRAVAHKDATVEWVDGNIGSKRTMKYPSIYLVGEGAHGEVLSIAFAGKGQEQD
                     TGGKAVHAASHTSSVITAKSISRAGGVATYRGLLKVNEGLHDVRSRVVCDALILDEKS
                     SSNTFPYMEINSDDVSIEHEARVSRISEEQLFYLMSRGMSEQEASVMIVNGFLDPLVK
                     QLPMEYAVELNRLIELEMTGTVG"
     gene            2311..3606
                     /gene="sufD"
                     /locus_tag="VFH83_00485"
     CDS             2311..3606
                     /gene="sufD"
                     /locus_tag="VFH83_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET6484861.1"
                     /translation="MSAARVEPSWMTADRDKAKARLESMKWPQTSEERWRRTDVSRLS
                     LDSYTVNSNGYSEAGLAATPREVDCGPHAGILRFDAGRCTECALNGFCKQRGVRLLPL
                     SLALEEFESPLRTLFAERLESLEDRFGAWHAWTWSHGAFLFVPRGVEVPEPFLLEMTE
                     AGSGIVSSPHVTVVLEEGARACVVQRLLESGTGSGSAGPGLLCNAGIDLSLGAASGVK
                     YFEEQRLGSRSLYFRETRAHLEKDASFAGLEVSLGSRVVKTDSEATMNGRGADVHLRG
                     LYFCGRDQHMDIASVQRHNAPSASSRAYYKGAVDSGGRTVFQGLIEVAKSASGTDAFL
                     TNRNLILGDAARADSIPTLKIGNNDVKCSHGSTTGRLSDEELFYLESRGLSRVEAREM
                     LVIGYFEELLDEAPEGMRESSIGAVRERLQQAARQERAA"
     gene            3609..3917
                     /locus_tag="VFH83_00490"
     CDS             3609..3917
                     /locus_tag="VFH83_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HET6484862.1"
                     /translation="MARWVRVASEGELETNLSVAVDGKPVAIFRTEDGVFALDDVCSH
                     EYSLLSEGEVFQGEVYCQKHGSRFSLKTGAVTGLPATQPVRAYAAKIEDGGVWIEWAS
                     "
     gene            3905..5179
                     /locus_tag="VFH83_00495"
     CDS             3905..5179
                     /locus_tag="VFH83_00495"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET6484863.1"
                     /translation="MGIVKTARDLLGGVEPRLLGGRTVTEVRGDFPILERLIGGKPLA
                     YLDNAATSQKPRAVLNAIRFYYENYNANVHRALHTLGEEATGAFERSRARVQTFINAR
                     SSAEIIFTRGTTESINLVASTWGQSVKPGDVVLLTQMEHHSNLVPWQMLSARTGCTLS
                     FIPIEPDGSLDLGAVERDWDDRVRLLSVAHVSNVLGTINDVKRLASIAHAHGALVLVD
                     GAQSVPHDRVDVRDLDCDFLAFSSHKMCGPMGMGVLYGRQTELEKLPPWMGGGEMIRA
                     VWPERSTWNDLPWRLEAGTPNVEGAIGLASAIEYLEGIGFADIAAWEAELSRYAAARL
                     SEVKELKLHGTAARKGAVFSFSLGSIHPHDVAQFLDREGIAVRAGHHCAQPLMRALGV
                     PATARASLYFYNTAQEIDRLAEGLEGALRFYA"
     gene            5176..5607
                     /locus_tag="VFH83_00500"
     CDS             5176..5607
                     /locus_tag="VFH83_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET6484864.1"
                     /translation="MSAFEDLYQEIVLDHYRSPRGKATLDHIPQSMVHENPTCGDSLK
                     LEVVMDPAGKVDHVLFDGKGCAISTASASLMTEAVKGLSVKEARERADAFIAALRGQA
                     PLETLEEMGDLAAFKGVASFPVRVKCATLAWHALLVTLPNP"
     gene            5620..6687
                     /gene="pdhA"
                     /locus_tag="VFH83_00505"
     CDS             5620..6687
                     /gene="pdhA"
                     /locus_tag="VFH83_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HET6484865.1"
                     /translation="MFECFDPREGKILSLLDETGTLTKAPDGWAVMDDEQALAGYRHM
                     VRARQADEWAVNLNRQGRMPTYVLCKGQEANAVGALMAMRQDDWFVPAYRELGAMIVR
                     GVGLTQIYRYWHGSEEGSRLPPETFHMMPIAVPVGSQVLHAVGIAYAERYRKSDRIAI
                     GFMGDGASSEGDFHEAANMAGVWKAGVVIFAQNNQWAISTPWNKQSASPTVAEKAFAY
                     GFEGIRVDGNDLFAVYTAVSMAARKARAGDGPTLVEGLTYRMGAHTTSDDPSRYRSAD
                     ETMNWQRRDPILRLELYLSRKGLLAAEEPRALREAALAEARAAFEEAEREGETAVEET
                     FSYLYADMPDLLALQMQRRME"
     gene            6684..7667
                     /locus_tag="VFH83_00510"
     CDS             6684..7667
                     /locus_tag="VFH83_00510"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET6484866.1"
                     /translation="MKVLTLLEAVNDALRLEMAADPGVLVFGEDVGKEGGVFRATVGL
                     QEQFGEERCFDTPLSESGIVGTAVGMAVAGLRPVTEIQFSGFAFCAFNQIANHAARMR
                     NRTRGALSAPMVIRMPYGGGIRAPEHHSESMEALFGHIPGLKVVVPSTPHDAKGLLIS
                     AIRDPDPVVFLEPARAYRAMRQEVPEESYTVEIGRAKVEAEGRHLTLVSYGAQMMEAR
                     QARAKLAADGQSVELIDLRTIYPFDASTVIGSVRKTGRLLVVHEGPRSFGVAAEIISI
                     VTEGAFDRLEAPPERLTGPDTVFPLPNGEHHYLITADLIESAARRVLAYEP"
     gene            7657..>8765
                     /locus_tag="VFH83_00515"
     CDS             7657..>8765
                     /locus_tag="VFH83_00515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET6484867.1"
                     /translation="MSPEGAIREFRFPDLGEGLTEGTVVELRVKPGDRVRQGDALAVV
                     ETDKVVTEIPAPVDGVVVEIPISPKQVVKVGEVVARFKVSQSEAASVVGRLDGGHREV
                     LVASLEGMPQPSVAGEAGPDGERPRASPIARKLAADAGIDLATLTGTGPGGRVLKRDV
                     IARGASAGATRLSTMRLAIATAMEKSQSIPSAVIHDFTLADPLLALRRDLNESLGVRV
                     GLLPFFVKAVALALNDAPLLGTWYYPERKEYETPASIDIGIAVDTEEGLVVPVVRKPA
                     TQKLASIQEQIERLAERAKSRTLLLEEIRGGAFTISNYGSAGGTYGKPMILPPQVGIL
                     GIGRVHQQPVVKDGAVVPAAVLPLSLVIDHRMVDGV"
BASE COUNT         1546 a         2783 c         3047 g         1389 t
ORIGIN      
        1 agacgctccg tgacgcgttc ggcgttccga cggtcgaggc ttccgtggtc tggacgcccc
       61 tctggggtcc ggagcggatg accgaagagg cgcgcgtggc gttgggctac cccatctgaa
      121 ggagagagaa tgacacacaa cctcagggtg aaggacctga aggcgaacat cggcgacaaa
      181 ccgatcctca caggtctcga tctggagatc cgcacggggg agattcacgc cctgatggga
      241 cccaacggat acgggaaaag caccctggcc aatgtgctga tggggcaccc ctcctacacc
      301 gtgaccggcg gaagcgtgac cctggacggg gaggatttcc tggcgctgaa gcccagtgag
      361 agggcgctgc ggggcatgtt tctcgcgttc cagtatccgg tggagattcc tggcgtgacc
      421 gtgggccggt tcatcaagcg ggccctggag ctgcgtgatg gcggcaaagg gatgaacgtg
      481 accgcctaca tcaaggagct ccgtcagagc atggacgtca tgggcattga ccaggtgttc
      541 atcaaccgct acgtcaacca gggcttttcc ggtggagaga agaagcgcat ggaggtgctg
      601 cagatgctga tgctcaagcc ctccctggcg ctcctggatg agacggactc cggcctggat
      661 atcgatgccc tgaaggtggt tgcccagggg gtcaaccgga tgcgggggga tggcttcggg
      721 gccctgatca tcacgcatta ccagcggatt ctcaccctgg tggtccccga cttcgtgcac
      781 atcatgtaca agggaaggat cgccacctcg ggtgggaagg aacttgtcga gcaactggag
      841 agcaagggct acgactgggt tcgacagcag ttcggaatcg aggaggaagc tcatgccagc
      901 gccagcgcag gccgttgaca agtcaccgac cgtagatatc ggagagtacc agcacggatt
      961 ctcgtatccg gaccagagcg tcttcaagac ccgaaagggc ctggatgagg aggtggtgaa
     1021 cgccatttcc caacacaagg gagagccgga ctggatgcgc cagttcaggc tgcgggcgct
     1081 ttccgcattc cgcaagaaga gcatgcccaa ctggggtggc gacctctcgg acctggactt
     1141 cgagaacctc tactactacg ccaagcccca gggcgagcag tcgcggacct gggaagaggt
     1201 gcccgccaac atcaaggaga ccttcgatcg attggggatc ccccaggccg agcggaagtt
     1261 tctggccgga gtcggagctc agtacgactc ggagatggtc taccacagca tcaacgccga
     1321 cctggccaag ctcggggtca tcttcacgga tcccgagacg gccgtgcggg agcatcccga
     1381 cctggtccgc aagtacttcg ggaccgtgat cccttacctg gacaacaagt tcgcggcgct
     1441 caacagcgcc gtctggtcgg gcgggagctt tgtctacgtc cccaagggcg tcaaggtgga
     1501 gatccccctg caggcgtact tccgcatcaa cgccaggagc ttcggccagt tcgagcgtac
     1561 cctcatcatc gccgatgagg ggagcttcgt gcactacatc gagggctgca cggcccccgt
     1621 gtacgccacc gagtcgctgc acagcgccgt ggtggagatc atcgccctgc ccggctctcg
     1681 cgtccgctac accaccatcc agaattggtc caacgacgtg tacaacctgg tgaccaagcg
     1741 ggccgtcgcc cacaaggatg ccacggtgga atgggtggac ggcaacatcg gctccaagcg
     1801 gaccatgaag tacccctcca tctacctggt tggcgagggc gcccacggcg aggtcctgtc
     1861 catcgccttc gcgggcaagg gacaggagca ggacaccggc ggcaaggcgg tgcacgccgc
     1921 cagccacacc agctccgtca tcacggccaa gtccatctcc cgcgccgggg gggtggccac
     1981 ctaccgcggc cttctcaagg tgaacgaggg tctgcacgat gtccgctccc gggtcgtctg
     2041 tgacgccctc atcctggatg agaagagcag cagcaatacc ttcccgtaca tggagatcaa
     2101 ctccgacgac gtgtccatcg agcacgaggc gcgggtcagc cggatcagcg aggagcagct
     2161 cttctacctg atgagccgcg gcatgagcga gcaggaggcc tccgtgatga tcgtcaacgg
     2221 cttcctggat cccctggtca agcagcttcc catggagtac gcggtggagc tgaaccggct
     2281 catcgagctt gagatgacgg ggacggtcgg atgagcgcgg cgcgcgtaga accgagctgg
     2341 atgacggcgg atcgggacaa ggcaaaggcg cgcctcgaat ccatgaagtg gccccagacc
     2401 tcggaggagc gctggcgccg aacggatgtg agtcgtctgt ccctggactc gtacacggtc
     2461 aactccaacg gatactccga ggcgggtctg gcagccacgc ctcgggaggt ggactgcggc
     2521 ccgcacgcgg gcatccttcg cttcgacgcg ggccgatgca ccgagtgcgc gctcaacggg
     2581 ttctgcaagc agcgcggcgt ccgcctgctt cccctctctc tcgcgctgga agagttcgag
     2641 agtccgctgc ggaccctgtt cgccgagcgg ctggaatccc tggaggatcg gttcggcgcg
     2701 tggcacgcat ggacgtggag ccacggggcg ttccttttcg tcccccgggg cgtcgaggtg
     2761 ccggagccgt ttctcctcga gatgaccgag gccgggtccg ggatcgtctc cagcccccat
     2821 gtcaccgtgg tgctggagga gggcgcccga gcgtgcgttg ttcagcggct cctcgagtcc
     2881 gggacgggtt cagggtcggc cggcccgggc cttctgtgca acgccggcat cgacctcagc
     2941 ctgggtgccg cgtccggtgt gaagtacttc gaggagcaga ggttggggag ccgttccctg
     3001 tacttccggg agactcgggc gcatctggag aaggacgcct ccttcgccgg gctggaagtg
     3061 agcctgggat ccagggtcgt caagacggac tccgaagcca cgatgaacgg caggggagcg
     3121 gacgtgcacc tgcgcggcct gtatttctgc ggccgcgacc agcacatgga catagcctcc
     3181 gtccagaggc acaacgcccc atccgcctcc agccgcgcct actataaggg cgccgtagac
     3241 tccggcggaa ggacggtctt ccaggggctg atcgaggtcg ccaagagcgc ctccgggacc
     3301 gacgcattcc tcaccaaccg aaacctcatc ctgggagatg cggcccgggc ggattccatc
     3361 ccgaccctga agatcggcaa caacgacgtg aagtgcagcc atggctccac cacgggccgt
     3421 ctcagcgacg aggagctctt ctacctggag agccggggcc tctcgcgggt ggaggcccgg
     3481 gagatgctgg tgatcgggta cttcgaggag ctcctggacg aggccccgga ggggatgcgc
     3541 gagagctcga tcggggcggt tcgggagcgc ctgcagcagg cggctcgcca ggagcgagcg
     3601 gcctgaccat ggcgcgatgg gttcgggtgg cctccgaggg cgagctggag accaacctga
     3661 gcgttgccgt ggacgggaag cctgtcgcga ttttccgcac ggaagacggc gtgttcgcgc
     3721 tcgacgatgt ctgttcccat gagtactctt tgctctcgga gggggaagtc ttccagggag
     3781 aggtgtactg ccagaagcac ggctcccggt tcagcctcaa gacgggggcg gtgacgggac
     3841 ttccggccac gcagccggta cgagcgtacg cggcgaagat cgaggacgga ggggtttgga
     3901 tcgaatgggc atcgtgaaga ccgccaggga cctgcttggc ggcgtagagc cccgcctgtt
     3961 gggcggacgg accgtcaccg aggtccgggg cgacttcccc atcctggagc gcctcattgg
     4021 cggaaaaccc ttggcgtacc tggacaacgc ggcgacgtcc cagaaacccc gcgcggtcct
     4081 gaatgccatt cgcttctatt acgagaacta caacgcaaac gtacatcgag ccctccatac
     4141 cctgggagag gaagccaccg gcgctttcga gcggagccgc gccagggtgc agaccttcat
     4201 caacgcccgg tcgtccgcgg agatcatctt cactcgtggc accacggagt ccataaacct
     4261 ggtggcctcc acctggggcc agagcgtgaa gccgggggac gtcgtgcttc tcacccagat
     4321 ggagcaccac tccaacctgg tgccctggca gatgctgagc gcccggaccg gatgcaccct
     4381 ctctttcatc cccatcgagc cggacgggag cctggacctg ggcgcggtgg agcgggactg
     4441 ggacgaccgg gtccgcctcc tctccgttgc ccacgtgtcc aacgtgcttg gcaccatcaa
     4501 cgacgtgaag cggctggcct ccatcgcgca cgcgcacgga gcgctggtgc tggtggacgg
     4561 cgcccaatcg gtgccgcacg accgcgtgga cgtgcgggac ctcgactgcg acttcctggc
     4621 gttctccagc cacaagatgt gcgggcccat gggcatgggc gttctgtacg gcaggcagac
     4681 cgagctggag aagctccccc cgtggatggg cggcggggag atgatccgcg ccgtgtggcc
     4741 cgagcgctcg acgtggaacg acctgccctg gaggctggag gcaggcacgc cgaacgtgga
     4801 aggggccatc ggtctggcat cggccatcga gtacctggag gggatcggct tcgcggacat
     4861 cgccgcgtgg gaagcggagc tttcccgcta cgcggcggcg cggctttccg aggtcaagga
     4921 gctgaagctc cacggcacgg cggcgcgcaa aggcgcggtc ttctccttca gcctgggctc
     4981 catccacccg cacgacgtgg cccagttcct ggaccgggaa gggatcgccg taagagccgg
     5041 gcaccactgc gcgcagccgt tgatgcgggc gctgggcgtc cctgccactg cccgggccag
     5101 cctctacttc tacaacaccg cccaggagat cgaccgtctg gcggaaggcc tggaaggcgc
     5161 cctgaggttc tacgcgtgag cgccttcgag gacctctacc aggagatcgt cctggaccac
     5221 taccggtccc cgcgcggcaa ggcaacgctg gatcacatcc cgcagagcat ggtccacgag
     5281 aacccgacct gcggcgattc gctcaagctc gaggtggtga tggatccggc gggcaaggtg
     5341 gaccacgtgc tcttcgatgg caagggctgc gccatcagca ccgcctccgc gtccctgatg
     5401 acggaggccg tgaagggcct ctccgtaaag gaggcccggg agcgcgcgga tgccttcatc
     5461 gctgccctgc gcgggcaggc gcccctggag acgctggagg agatgggtga cctggcggcg
     5521 ttcaaaggcg tcgcgagctt cccggttcgg gtcaagtgcg cgacgctggc ctggcatgct
     5581 ttgctggtga cgctcccaaa tccctgaagg agttccctca tgttcgaatg tttcgaccct
     5641 cgggagggga agatcctttc cctcctggat gaaaccggca ctttgaccaa agccccggat
     5701 ggctgggccg tgatggacga cgagcaggcc ctggcgggct accgccacat ggtccgcgcc
     5761 cggcaggccg acgagtgggc cgtgaacctg aaccgccagg gccgaatgcc cacctacgtg
     5821 ctgtgcaagg ggcaggaggc caacgcggtt ggggcgctca tggcgatgcg ccaggacgat
     5881 tggttcgtgc cggcgtaccg cgagctgggc gcgatgatcg tccgcggggt cgggctgacg
     5941 cagatctatc gctactggca cgggagtgag gagggcagcc ggctgccgcc ggagaccttc
     6001 cacatgatgc ctatagcggt gccggtggga agccaggtcc tgcacgcggt cggcatagct
     6061 tacgccgagc gttaccgaaa gagcgatcgc atcgccatcg gcttcatggg cgatggggcc
     6121 tcgtcggaag gggacttcca cgaagccgcc aacatggccg gcgtgtggaa agccggcgtg
     6181 gtgatctttg cccagaacaa ccagtgggcc atctccacgc catggaacaa gcagagcgcc
     6241 agccccaccg tcgcggagaa ggcctttgcc tacggcttcg agggaatccg ggtggatggc
     6301 aacgacctct tcgccgtcta cacggccgtg agcatggccg cgcgcaaggc ccgcgccggt
     6361 gatggaccca ccctcgtcga gggcctcacg taccgcatgg gcgctcacac cacctcagac
     6421 gatccttccc gatatcgcag cgccgatgag accatgaact ggcagcggcg ggaccccatc
     6481 ctgcggctgg agctctacct ctccaggaag gggctcctgg ccgccgaaga gccgcgcgcg
     6541 ctgcgggaag cggcgctggc ggaagcccgt gccgccttcg aggaggccga gcgcgaaggg
     6601 gagaccgccg tggaggagac cttcagctac ctgtacgcgg acatgccgga cctgctggcg
     6661 cttcagatgc agaggaggat ggaatgaagg tcctcaccct gctggaagcg gtgaacgatg
     6721 cgcttcgctt ggagatggcg gcggatcccg gcgtgctggt cttcggggaa gacgttggca
     6781 aggagggcgg agtcttccgc gccacggtcg ggctccagga gcagttcggg gaggagcgct
     6841 gcttcgacac gcccctctcg gagtccggga tcgtcggaac ggcggtcggg atggccgtgg
     6901 cggggctgcg tccggtcacg gagatccagt tctcggggtt cgccttctgc gcgttcaacc
     6961 agatcgcgaa ccatgcggcc cgaatgagga accgcactcg cggcgcgctc tccgctccca
     7021 tggtgatccg catgccctac ggcggcggga tccgggcgcc ggagcaccac tcggagagca
     7081 tggaggcgct cttcggccac atcccggggc tcaaggtcgt ggtcccttcc acgccgcatg
     7141 acgccaaggg cctcctgatc tccgcgattc gcgacccgga ccccgtggtc ttcctggagc
     7201 cggcgcgcgc ctaccgcgcc atgcgccagg aggtgccgga ggagagctac acggtggaga
     7261 tcggccgcgc caaggtggaa gccgagggcc gtcacctgac gctggtctcc tacggggcgc
     7321 agatgatgga ggcgcggcag gcgcgcgcca agctcgcggc ggacgggcaa tccgtggagc
     7381 tgatcgacct ccgcacgatc taccccttcg atgcctccac ggtgatcggc tcggttcgca
     7441 agacgggccg cttgctggtg gtgcacgagg gaccgagaag cttcggcgtg gcggcggaga
     7501 tcatctccat cgtgacggag ggggccttcg accgcctgga ggcgccaccg gagcggctca
     7561 ccgggccgga caccgtgttc ccgcttccca acggggagca tcactacctg atcaccgcgg
     7621 acctcatcga gtcggccgca aggagggtcc ttgcctatga gccctgaggg agccatccgg
     7681 gagttccggt tccccgatct cggtgagggc ctcaccgagg gtaccgtggt cgagcttcgc
     7741 gtgaagccgg gtgaccgcgt gcgccagggg gacgccctgg cggtggtgga gacggacaag
     7801 gtggtgacgg agatcccggc cccggtagac ggggtggtcg tggagatccc gatctccccg
     7861 aagcaggtcg tgaaggtcgg agaggtggtg gcccggttca aggtctccca gtccgaggca
     7921 gccagcgtgg tgggccgcct ggacggaggg caccgagagg tcctggttgc cagcctggag
     7981 ggcatgccgc agccgagcgt tgcgggagaa gcgggaccgg atggagagcg gccccgtgcc
     8041 tcgcccatcg cccgaaagct tgccgcggac gcgggcatcg acctggcaac gctcaccggg
     8101 accggccccg gcgggcgggt cctgaagcgg gacgtgatcg cccgaggcgc ttccgcgggc
     8161 gccacccggc tcagcaccat gcgccttgcc atcgcgacag ccatggagaa gagccagtcc
     8221 atcccgtcgg cggtcattca cgacttcacg ctggcagacc cgctcctggc ccttcggcga
     8281 gacctcaacg agagcctggg ggtccgggtg gggctgctcc cgttcttcgt gaaggcggtg
     8341 gccctggcgc tcaacgacgc gccgctcctg ggcacctggt actaccccga gcgcaaggag
     8401 tacgagacgc cggcatccat cgacatcggc atcgcggtgg acaccgagga gggcctggtg
     8461 gtgcccgtgg tccgaaagcc ggcaacgcag aagctggcga gcatccagga gcagatcgag
     8521 cggctggcgg agcgcgccaa gtcacggacg ctcctgctgg aggagatccg cggcggcgcg
     8581 ttcaccatct ccaactatgg gtccgccggc ggcacctacg ggaaacccat gatcctgccg
     8641 ccccaggtgg gcatcctggg gatcgggcgc gtgcaccagc agcccgtggt caaggacggc
     8701 gccgtcgtgc cggcggcggt gctgccgctg tccctggtga tcgaccaccg gatggtggac
     8761 ggcgt
//
LOCUS       DASRZS010000021         4069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3474347, whole genome shotgun sequence.
ACCESSION   DASRZS010000021 DASRZS010000000
VERSION     DASRZS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3474347"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFH83_00520"
     CDS             <1..471
                     /locus_tag="VFH83_00520"
                     /inference="COORDINATES: protein motif:HMM:NF016138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HET6484868.1"
                     /translation="GCVAGQLDVALLGATEVDLDFNVNVNTHSDGILLHGIGGHSDAA
                     AGAGCCMITVPSFRKRIPMVRERVTTVSCPGELVDVVVTERGIAVNPRREDLADRARH
                     AGLPLVTLADVMRDVRAFTGVPQPPRFRDRVVALIEWRDGTTLDVVREIEPSAP"
     gene            507..2351
                     /locus_tag="VFH83_00525"
     CDS             507..2351
                     /locus_tag="VFH83_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HET6484869.1"
                     /translation="MEPTLSSLARTTHLLPGEVATRRDRNRAYLMRLTARNLLLSHYL
                     EAGLIKINYKPEGIHWGWDAPTSEIRGTVVGHWLSAAARLWEETGDHELKARADFIVS
                     ELARCQAENGGEWFFAIPEKYLYWIKRGKHAWAPQYVCHKNMMGLLDMHRYAGNTQAL
                     EILTKAARWFHRFTDDVTREQMSAMMSLEETGGIMELWADLYAVTKDAAHLELMQRYE
                     RPELYEPLLRGEDVLTNLHANMTIPEMHGAARAYEVTGEDRYRRIVESYWDLAVRRRG
                     AFATGGQTSGEVWTPPDRQSARLGELNQEHCTVYNLMRLAEYLLRWTGSAEYADYWER
                     NLYNGILAQGFWEGRAMDMLTEPHEPTRGLVAYYLPLAAGSTKKWGSETEHFWCCHCT
                     LLQANANHREAIYYQNERALVISQYLPSRVELTVDGVRIAVSQRFDPRTGDILKIHPV
                     SRAVPSRESSVRVAVLVEPERPVELSLRFRLPWWLAGRASVTIDGEESAYTEDAGYGA
                     IRRTWSRTELILTLPKRLSCWPLPDRPDTVAFLDGPVALAGLVGEEREMVGDISQPLS
                     LLAPDDERHWSSWQTGWKTVNQPIGWRFKPLYEIGNERYTVYFPVRRR"
     gene            2445..3923
                     /locus_tag="VFH83_00530"
     CDS             2445..3923
                     /locus_tag="VFH83_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6484870.1"
                     /translation="MRHDTTSREGYEWVALSVTTIGALLASIQGSALIIGLPDILAKL
                     GADFLTMIWVLLAYLLVTTAFVPVIGRLADMFGRKRLYNAGFAIFSLGSLFAGLAQAR
                     FHGWDLVLARVVQGIGGALLFANSAAIVTDAFRHGRIGLGLGVNQIAVAAGFILGPVV
                     GGLLASIDWRLIFLINVPLGLFGTLWGIRNLREPEKLPGGQHFDPWGSVTFTVGLAAL
                     LLGLSITAFPAGPAFLPYALIAFAVVVLTAFALIERRVPEPMLDFQLFRHRLFSFANL
                     AGGLNGLARGAVLFLLTFFLQGPYGKNPLQAGILTAPFGIAFVAVGPLSGSLSDRHGS
                     RGLATAGLLVSSVGLLGLATITATTSYWVLAVFMVLMGGGSGLFSSPNQNAIMSSVVP
                     ERRGIASATGVMFGNTGQMFSIAIAFPLVLSHIPQDVMYKIFLYGGGMQGVPTVLQTF
                     EKGLHQAFLVSFAITIVAAAASFLRPSKEEPIAARADLEEQP"
BASE COUNT          645 a         1373 c         1324 g          727 t
ORIGIN      
        1 gggtgcgtgg cgggccaact cgacgtggcg ctcctgggag ccaccgaggt ggacctggac
       61 ttcaacgtga acgtcaacac gcactcggat gggatcctgc tgcacgggat cggcggacac
      121 tccgacgcgg cagcgggcgc cggctgctgc atgatcaccg tcccgtcctt tcgaaagcgg
      181 atccccatgg tccgtgagcg ggttaccacg gtatcctgtc ctggagagct ggtggacgtg
      241 gtcgtcaccg agcggggcat cgcggtcaac ccccgacggg aggacctggc ggaccgtgcc
      301 cgccacgccg ggcttcccct ggtgacgctg gcggatgtga tgcgggacgt gcgcgccttc
      361 acgggcgttc cccagccgcc ccgatttcgg gaccgcgtgg tcgccttgat cgaatggcgc
      421 gatgggacca cgctggatgt ggtacgggag atcgagccga gcgcgccgtg aacacggcgc
      481 cggcgcggct ccctcgggag gtgctcatgg aaccgaccct gtcatccctg gctcgaacga
      541 cccatctcct gccgggtgag gtagcgaccc gccgtgaccg caatcgcgcg tacctgatgc
      601 ggctgacggc ccgcaatctc ctcctctctc actacctgga ggccggtctc atcaagatca
      661 actacaagcc cgaagggatc cactggggat gggacgcgcc caccagcgag atccggggca
      721 ccgtggtggg ccactggctc agcgcggcgg cccggctctg ggaggaaacc ggagatcacg
      781 agctcaaggc ccgcgcggac ttcatcgtct ccgagctggc gcgctgccag gcggagaacg
      841 gcggggaatg gttcttcgcg atcccggaga agtacctcta ctggatcaag cggggaaagc
      901 acgcctgggc tccgcagtac gtctgtcaca agaacatgat gggccttctg gacatgcacc
      961 ggtacgcggg aaacacccag gcgctcgaga tcctgaccaa ggctgcccgt tggttccacc
     1021 gattcacgga cgacgtcacc cgggagcaga tgtccgccat gatgagcctg gaggaaaccg
     1081 gcgggatcat ggagctgtgg gcggacctct acgcggtgac gaaggacgcc gcgcacctcg
     1141 agctcatgca gcgctacgag cggcccgagc tctacgagcc gcttctccga ggtgaggacg
     1201 ttctcacgaa cctgcacgcc aatatgacga tccccgagat gcatggcgcg gcgcgggctt
     1261 acgaggtcac cggcgaggac cgataccgca ggattgtcga gagctactgg gaccttgcgg
     1321 tccgccgccg cggagccttc gcgaccggcg gccagacctc cggagaggtc tggacccctc
     1381 ccgaccggca gtccgctcgc ctgggcgagc tgaaccagga gcactgcacg gtctacaact
     1441 tgatgaggct ggccgagtat ctcttgcggt ggacggggag cgcggagtac gccgactact
     1501 gggagcgcaa cctgtacaac ggcatcctgg ctcaaggatt ctgggaaggc agggccatgg
     1561 acatgctcac cgagccgcat gagccgacga gaggactggt agcctactat ctccccctgg
     1621 ccgccggttc caccaagaag tggggctccg agacggagca cttctggtgc tgccactgca
     1681 ccctgcttca ggccaacgcg aaccatcggg aggcgatcta ctaccagaat gagagggctc
     1741 tcgtcatttc gcagtacctc ccatcccggg tggagctcac ggtggacggg gtccggattg
     1801 ccgtctccca gaggttcgac ccgcgcaccg gcgatatcct gaagatccat ccggtgtccc
     1861 gcgccgtacc ttcccgcgaa tcgtcggtac gggtggcggt gctcgtggag ccggagcgtc
     1921 cggtcgagct atccctcagg ttccggctgc catggtggct cgccgggcga gccagtgtca
     1981 ccatagatgg cgaagaatcg gcttacacgg aggatgccgg atacggcgcc atccggagga
     2041 cctggtcccg gaccgagctg atcctgacct tgcccaagcg cctctcctgc tggccgctgc
     2101 ccgaccggcc cgacaccgtt gcgttcctgg acggccccgt ggcgctggcg gggctggtcg
     2161 gtgaagagcg ggagatggtc ggggacattt cgcagcccct ctccctgttg gccccggacg
     2221 acgagcgcca ctggtcgtcg tggcagaccg gatggaagac cgtgaaccag ccgatcggct
     2281 ggcgcttcaa gccactctac gagatcggca acgagcgcta caccgtctac ttcccggtgc
     2341 gccgccgctg agcctccata gccggtccgg ccgattgacc tctaccggcc cccatcgcta
     2401 ccttttaccc accgcactcg aacgagtctg gaaaacgggg gatcatgcgc cacgatacta
     2461 cctcacgaga aggctacgaa tgggtagcgc tgtcggtcac gaccatcggc gcgctgctgg
     2521 cgtcgatcca gggctcggcg ctcatcatcg gacttcccga catcctggcc aagctgggcg
     2581 cggacttcct caccatgatc tgggtgctcc tggcctacct cctggtgacc accgcgttcg
     2641 tcccggtgat cggcaggctg gcggacatgt tcggccgcaa aaggctctac aacgccgggt
     2701 tcgccatctt ctccctcggt tccctgttcg ccggcctggc ccaggcgcgg ttccacggct
     2761 gggacctcgt ccttgcccgc gtggtgcagg ggatcggcgg ggcgctcctt ttcgccaaca
     2821 gcgccgccat cgtgacggat gcgtttcgac acgggaggat cggtctgggg cttggcgtga
     2881 accagatcgc cgttgcggct ggtttcatcc tgggtccggt ggtgggaggg ctcctggcgt
     2941 cgatcgactg gaggctcatc ttcctgatca acgtccctct gggcctgttc ggaaccctgt
     3001 ggggaatccg taacctccga gaaccggaga agttgcccgg tgggcagcac ttcgatccct
     3061 ggggcagcgt caccttcacc gttgggctgg cggcgctcct cctgggacta tcgatcacgg
     3121 cattcccggc gggccccgcg ttcctcccgt acgcgctcat cgcgttcgcc gtcgtggtcc
     3181 tgacggcctt cgcgctgatc gaacgtcgcg tgcccgagcc gatgctggat ttccagctct
     3241 tccggcatcg cctgttctcc ttcgcgaatc tcgccggggg tctgaatggc ctggcccggg
     3301 gcgccgtgct cttcctcctg acgttctttc tccagggtcc gtacggaaag aacccgcttc
     3361 aggccggtat cctgaccgcg ccgttcggga tcgcctttgt cgccgtgggt cccctctcgg
     3421 gttcgctttc tgaccggcac gggtctcgcg gtctggccac ggcggggctg ctggtgtcca
     3481 gcgtcgggct cctgggcctg gccaccataa cggcgaccac cagctactgg gtgctcgcgg
     3541 tcttcatggt cctgatgggc ggcggctcgg ggctcttcag ctccccgaac cagaacgcaa
     3601 tcatgtcctc cgtggtcccc gagcggcgag gcattgcctc ggccaccggc gtcatgttcg
     3661 ggaacaccgg gcagatgttc agcatcgcca tcgcgttccc gctcgtcctg agccacatcc
     3721 cccaggacgt catgtacaag atcttcctgt acggcggggg gatgcagggg gttcccacgg
     3781 tgctgcagac tttcgagaag ggcctccacc aggccttcct cgtctcgttc gccatcacga
     3841 tcgtcgccgc cgcggcgtcg ttcctccggc cttccaagga agagccgatt gcggcccgag
     3901 cggacctgga ggagcaacca tgaacgtccc tgcattcggc gcccaccccg acgacgcgga
     3961 gcggccaagc tctacccggc ccaggtcgga tcgtgagcga gttccgcggg cccctttgcc
     4021 gtagaagctc ctggctcggg ccgcgccgac cgaaaactct ccacgttcc
//
LOCUS       DASRZS010000022         6557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5014170, whole genome shotgun sequence.
ACCESSION   DASRZS010000022 DASRZS010000000
VERSION     DASRZS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5014170"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VFH83_00535"
     CDS             complement(<1..793)
                     /locus_tag="VFH83_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET6484871.1"
                     /translation="MKVIVCIKQVPDTTNVRINPETNTLVREGVQSIINPFDVYALEE
                     GVRIKEKHGGTVTVITMGPPQAAEALREAIGLGADNAYLISDRAFAGADTLATAFALS
                     CAIRKLGAGGAPGSEGIADIILMGRQAIDGDTGQVGPGVAENLGISHVTDIRKIEEIK
                     DGRIVVERLLEEGYARIGARLPVVLTVVKEINTPRLPSLKGKMAARKAEIPVLKAVDI
                     GAETERLGLNGSPTQVMRIFTPPKPSGGKRFTGEPSATVTQLIEEL"
     gene            complement(803..1963)
                     /locus_tag="VFH83_00540"
     CDS             complement(803..1963)
                     /locus_tag="VFH83_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6484872.1"
                     /translation="MNYFLTEDQKMIKELAAKLAADKIAPIRAELDESGEFPWEIVKL
                     LAQSDLFRVFIPTEYDGLGGGIFEMSLVVEELSKACGGIALSYAASGLGTIPIILFGN
                     EEQKKKYLPRLAAGEILAAFGLTESGAGSDAAGIKTTAKPDGDCFVLNGTKQWITNGG
                     EASIYTVIALTDPTRGVRGSSALLVEKDHPGFTFGKKENKMGIRASATRELVFQDCRV
                     PKKDILGKEGMGFLVAMRTLDQSRPGVAAQALGIAQGALDEALAYSRERTQFGKPISS
                     FQGLQFILADMATQVEAARALTYAAARSIDAGDKDVSRMSAMAKVFASDTAMKVTTDA
                     VQVFGGYGYMKEYPVEKMMRDAKITQIYEGTNQIQREVIALNLIKESVRAKK"
     gene            complement(2111..3277)
                     /locus_tag="VFH83_00545"
     CDS             complement(2111..3277)
                     /locus_tag="VFH83_00545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6484873.1"
                     /translation="MDSPSEATVAAVSSVEVRPVRTQRERRVFLTFPWRVYANDPLWV
                     PPLLSERASTIDPARGVFWKRGEAELFVAWKDGEAVGTICAAEDKALNGHLGGRECIF
                     GFFECVEDTVVARALIDRAATWARARGLVTLGGPFNLDYEDSYGVLVEGRDRPPVILC
                     GHTPVYYQGFLEDYGFVPLRGDNLAYEIPLSASSAALQRTALMAERIRSKGWLKIRTP
                     DLDRWQEEVDVVLELLNRSLAHLTDFRPYEREAVEAMVGPFRSIADADLILFVQVDGK
                     TVGWFPGVPNMNEVLIHLNGLRYPWDMARALRWMRYRPRCLSIKSVLILPEYWGSGAA
                     LLLIDEMARRARAKGYAWADLSLTSADNPYTPELATRMGARIYKRYRVYGKRVA"
     gene            3430..3927
                     /gene="lspA"
                     /locus_tag="VFH83_00550"
     CDS             3430..3927
                     /gene="lspA"
                     /locus_tag="VFH83_00550"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET6484874.1"
                     /translation="MLPFILSAGIIVLDQITKLIVVRTVPEGSGVRVLGDFFRIVDVR
                     NPAISFSIGRSLQAGTRHTLFTLLPLAVILVLLLYYLLTRDPLTRFQRWTFGALVGGG
                     LGNYVDRIFRPSGVVDFLDFKFYGLLGLERWPTFNVADTTVVVSGILLFISFLIPTRK
                     PGGTS"
     gene            3924..4196
                     /locus_tag="VFH83_00555"
     CDS             3924..4196
                     /locus_tag="VFH83_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484875.1"
                     /translation="MSSNGKMVLFFLCATVFNIALMVVFVAVFIIGIGLALGSKPNPV
                     TFQILVFVGFVASIVLTFLLYGWVMKKVTARFQLEKHIPQLFKRKK"
     gene            4198..4713
                     /locus_tag="VFH83_00560"
     CDS             4198..4713
                     /locus_tag="VFH83_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET6484876.1"
                     /translation="MDFLQLVRERESVRSYDPTRPVERAVLQRILEAAHYAPSAANRQ
                     PWRFMVICSREVLAKVRRCYDKPWFQDAPTVLAMVGKVGEAWTRQDGWNSIETDLTIA
                     MDHLILAAESEGVASCWVAAFDPILLRSSLSLAADERVYALTPLGYPRPGYASKTPKQ
                     RKTLEEIVRYV"
     gene            complement(4753..6417)
                     /locus_tag="VFH83_00565"
     CDS             complement(4753..6417)
                     /locus_tag="VFH83_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484877.1"
                     /translation="MAAGAPDSLDPDLAALLDLDSGGEKTPSEPEPSLDLKGGEPDFH
                     TLFGEEKPPARGKPDEVEVTRNHFPAITKIEGDPKPFFQDKDYYRKALSGEGDTGPRV
                     HELLGKYMKAEDPEEKSHLRAQLISAFWDLGSSVANKSGHEMPIPKRLLLRFGILSPG
                     FLTPELQQMVSRIIFDNDTGEPVFYLDEWLDRIAKGQIRPSAADEVKAKTRDAGVKVK
                     EVLEKKRGLRDSEITLYKAKVSQLEEREAQLRAQVELLAHHESLDGYTNLKAPYTPAQ
                     KGTLASIQELAKQLSTLDGQMASSFAELQAFDKDLENLSRKSDAAADGGVDLKAASNE
                     YACIRQMAKMCVGRKGNHFPIFHSQFVRPNIREIATRENVIKEMSAVEALDPGLFQRT
                     HKNQTTRIVPNIILIPSYGDTGVCWEPFERFNRATSRGRVAIPLFPKSLPVAVLTALA
                     DLRWQVAKEKAQYYWMEEGITGKYYQWFSERKLRGDVREYFIRDYLLWINKESQGMQK
                     LDKDVRGIFWRMLPFPQPIKDALRNRGFVYNELYKKDQNIAKSDGY"
BASE COUNT         1047 a         2108 c         2105 g         1297 t
ORIGIN      
        1 cgagctcctc tatgagctgc gtgacggtgg ccgacggttc accggtgaac cgcttgccgc
       61 cggacggctt gggcggggtg aagatccgca tgacctgcgt gggggagccg ttcaggccca
      121 ggcgctcggt ctcggcgccg atgtccactg ccttcaggac ggggatctcc gccttgcgcg
      181 cggccatctt tcctttcagg gaaggaaggc gcggggtgtt gatctccttg accacggtga
      241 gcacgacggg cagccgggca ccgatccgcg cgtagccctc ctccaggagg cgttccacca
      301 cgatcctgcc gtccttgatc tcctcgatct tgcggatgtc cgtgacgtgg ctgatcccca
      361 ggttctccgc cacaccgggc cccacctggc ccgtgtcccc gtcgatggcc tggcggccca
      421 tcaggatgat gtcggcgata ccttcggaac cgggggcacc gcccgcgccg agcttgcgga
      481 tggcgcagga gagcgcgaac gccgttgcca gcgtgtcggc gccggcgaac gcgcggtccg
      541 agatgaggta ggcgttgtcc gcgcccagcc cgatggcctc ccgaagcgcc tccgcggcct
      601 ggggcggtcc catcgtgatg accgtgaccg tgccgccgtg tttctccttg atccgcacgc
      661 cctcttccag ggcgtagacg tcgaagggat tgatgatgga ctgcacgccc tcgcggacca
      721 gggtgttggt ctcggggttg atgcggacgt tggtggtgtc cgggacctgc ttgatgcaaa
      781 cgatgacctt cacgaccggc ctctacttct tcgcgcgaac gctttccttg atgaggttga
      841 gggcgatcac ctcgcgctgg atctggttgg tgccctcgta gatctgcgtg atcttggcgt
      901 cccgcatcat cttctccacc gggtattcct tcatgtagcc gtatccgccg aagacctgca
      961 cggcgtccgt ggttaccttc atggcggtgt cggaggcgaa caccttcgcc atggcggaca
     1021 tccgagacac gtccttgtcc cccgcgtcga tgctgcgggc cgccgcgtac gtcagggcgc
     1081 gcgccgcctc cacctgcgtg gccatgtcgg ccaggatgaa ctgcagtccc tggaacgagg
     1141 agatgggctt gccgaactgc gtgcgctccc gggagtaggc cagcgcttcg tccagcgctc
     1201 cctgggcgat ccccaacgcc tgggcggcga cgcccgggcg ggactggtcc agggtcctca
     1261 tggcaaccag gaatcccatg ccttccttgc ccaggatgtc cttcttgggc acgcggcagt
     1321 cctggaagac gagctcccga gtggccgagg cgcggatgcc cattttgttc tccttcttgc
     1381 cgaaggtgaa ccccggatgg tctttctcca ccaggagggc cgaggagccg cgcacaccgc
     1441 gggtcgggtc cgtgagggcg atgaccgtat agatggaggc ctcgccgccg ttggtgatcc
     1501 actgtttggt gccgttgagg acgaagcagt ccccatccgg cttggcggtg gtcttgatgc
     1561 ccgcggcgtc gcttccggcg ccggactccg tgagcccgaa ggcggccagg atctctcccg
     1621 cggccaggcg cggcagatac ttcttcttct gctcctcgtt gccaaagagg atgatgggga
     1681 tggtgccgag gcccgaggcc gcgtacgaca acgcgatccc tccgcacgcc ttggagagct
     1741 cctccaccac gaggctcatc tcgaagatgc ctccgcccag gccgtcgtac tcggtaggga
     1801 tgaaaacacg gaacaggtcg gactgggcga gcagcttcac gatctcccag gggaactccc
     1861 cgctctcgtc gagctccgcg cggatggggg cgatcttgtc cgccgcgagc ttggcggcca
     1921 gctccttgat catcttctga tcctcagtca aaaagtaatt catggcaggc cttcctccct
     1981 cgttgggatc ccggaaatct ttacaaaatt ggtatggatt gagtctggac catagggcgg
     2041 tcggagtcaa gggggggatg gaagaggcat accgaaccgc cgcggctggc ccctggtcag
     2101 gcgcatgggc tcaggcgacc cgcttcccgt acactcggta ccgcttgtag atccgggcgc
     2161 ccatgcgcgt ggccagctcc ggggtgtagg ggttgtcggc ggaggtcaag gagaggtccg
     2221 cccaggcgta gcccttggcc ctggcgcgcc ttgccatctc gtcgatgaga agaagggcgg
     2281 ccccgcttcc ccagtactcc ggcaggatca gcacgctctt gatggagaga caccgggggc
     2341 gataccgcat ccagcggagc gcccgcgcca tatcccaggg gtaccgaagg ccgttgaggt
     2401 ggatcagcac ctcgttcatg ttcgggacgc cgggaaacca tccgacggtc tttccatcga
     2461 cctggacgaa caggatgagg tcggcgtccg cgatggaccg gaagggcccg accatggcct
     2521 ccaccgcctc gcgctcgtac gggcggaaat ccgtcaggtg agcgagggac cgattcagca
     2581 gctccaggac gacgtccacc tcctcctgcc accgatcgag atccggcgtt cggatcttca
     2641 gccacccttt gctgcggatc cgctccgcca tgagcgcggt acgctggagg gccgcggagg
     2701 acgcgctcag cggaatctcg tacgccaggt tgtctccccg aagcggcaca aagccgtagt
     2761 cctccaggaa cccctggtag tagaccggcg tgtggccgca caggatcacg ggaggccgat
     2821 cccggccttc cacgagcacg ccgtaactgt cttcgtagtc gaggttgaag gggccgccca
     2881 acgtgacaag accgcgagcc cgtgcccacg tggcggcccg gtcgatgagc gcgcgggcaa
     2941 cgaccgtgtc ctccacgcac tcgaagaatc cgaagatgca ctcccgtccg cccaggtgac
     3001 cgttgagcgc cttgtcctcg gccgcgcaga tggtccccac ggcctcccca tccttccagg
     3061 ccacgaagag ctcggcttct ccgcgcttcc agaacactcc gcgggccgga tcgatcgtcg
     3121 aagcgcgctc cgaaaggagc ggtggcaccc acaacggatc gttggcataa acgcgccatg
     3181 ggaaggtgag gaaaacccga cgctcgcgtt gggttcgcac gggacggacc tcgacacttg
     3241 agaccgctgc caccgttgcc tccgaaggtg aatccatgat atcctttaga tcgtactttt
     3301 tccacggaat tgttcgtggt tcgagcggtg gtccaatttc tccaatggag gaacgggcct
     3361 ccggcaaacc gtgacgcgct gggttcttgg atgattctca acacgaggaa gcgagatata
     3421 cgttcccggt tgctcccttt tatcctctcg gccgggatca tcgtcctgga ccagatcacc
     3481 aagctgatcg tggtccgcac cgtccccgag ggcagcgggg tccgcgtact gggagatttc
     3541 ttccggatcg tggacgttcg caacccggcc atctccttca gcatcgggcg ctcccttcaa
     3601 gccgggacgc gccacacgct gttcacgctg ctgccgcttg ccgtgatcct ggtgctgctc
     3661 ctctactacc tgctcacccg ggatccgctc acccgattcc agcgctggac gttcggcgcc
     3721 ctggttggcg gcgggctggg caactacgtg gatcggatct ttcggccctc cggagtcgtc
     3781 gacttcctgg acttcaagtt ctacgggctt ctgggtctgg aacgctggcc gacgttcaac
     3841 gtggcagaca cgacggtggt ggtttcaggc atcctgctct tcatctcctt tctgattcca
     3901 acgcgaaaac cgggagggac ctcgtgagct ccaacggcaa gatggtgctg ttctttctgt
     3961 gcgccacggt cttcaacatc gccctgatgg tggtgttcgt ggccgtgttc atcatcggca
     4021 tcggtctggc cctggggtcc aagcccaacc ccgtgacctt ccagatcctg gtgttcgtgg
     4081 gcttcgtggc ctccatcgtg ctgaccttcc tcctctacgg atgggtcatg aagaaggtca
     4141 cggcccggtt ccagctcgaa aagcacatcc ctcaactctt caagaggaag aagtgacatg
     4201 gatttcctgc agctcgtccg ggaacgagag agcgtccgca gctatgaccc gacgcgaccg
     4261 gtggagcgcg cggtgctgca gcggatcctc gaggcggcgc actatgctcc gtcggcggcc
     4321 aaccgccagc cctggcgttt catggtgatc tgctcccggg aggtcctggc gaaggtccgc
     4381 cgctgctacg acaagccgtg gttccaggac gctccgacgg tcctggccat ggtgggcaag
     4441 gtgggggagg cgtggacccg acaggatggg tggaactcca tcgagacgga cctgaccatc
     4501 gccatggacc atctgatcct ggccgcggag agcgagggcg tggcaagctg ctgggtagcc
     4561 gcgttcgatc ccatcctcct tcggtccagc ctctctctgg ccgcggatga gcgggtctat
     4621 gcgctcacgc cgctggggta cccgcgcccg ggatacgcca gcaagacgcc gaagcagcgc
     4681 aagacgctgg aagagatcgt tcgatacgtc taggggggat tgccaccgcc gcggtgtacg
     4741 ccgcgccgtc cgtcaatacc cgtcggactt ggcgatgttc tggtccttct tgtacagctc
     4801 gttgtaaacg aacccgcggt ttctgagcgc gtccttgatg ggctggggga acggcagcat
     4861 gcgccagaaa atcccgcgca cgtccttgtc cagcttctgc atcccctggc tttccttgtt
     4921 gatccagagc aggtagtccc ggatgaaata ctcccggacg tccccgcgca gcttgcgctc
     4981 gctgaaccac tggtagtact tgcccgtgat cccctcttcc atccagtagt actgggcctt
     5041 ctccttggcc acctgccacc gtaggtccgc gagcgcggtc agcacggcga cgggcaggct
     5101 cttggggaac aaggggatgg cgactcgacc acggctggtc gcacggttga agcgctcgaa
     5161 cggctcccag cagacccccg tgtcaccgta ggacggaata aggatgatgt tggggacgat
     5221 gcgcgtggtc tggttcttgt gggtgcgctg aaagagcccg ggatccagcg cctcgacggc
     5281 ggacatctcc ttgatgacgt tctcccgggt ggcgatctcc cggatgttcg gacggacgaa
     5341 ctgggagtgg aagataggga agtggttccc ctttctccct acgcacatct tcgccatctg
     5401 gcggatgcag gcgtactcgt tcgacgccgc cttcagatcc acgccgccgt ccgccgcggc
     5461 gtcggacttc cgggagaggt tctccaggtc cttgtcgaat gcctggagct ccgcaaagga
     5521 ggacgccatc tggccgtcca gcgtggagag ctgcttggcc agctcctgga tgctggcaag
     5581 ggtgcccttc tgcgccggcg tgtacggcgc cttgaggttg gtgtagccgt ccaggctctc
     5641 gtggtgcgcc aggagctcca cctgcgcccg aagctgggcc tcgcgctcct ccagttggga
     5701 caccttcgcc ttgtagagcg tgatctcgct gtcgcggaga ccgcgtttct tttccaggac
     5761 ttccttgacc ttgacgccgg catcccgggt cttggccttg acctcgtccg cggcggaagg
     5821 tcggatctgg cccttggcga tcctgtccag ccattcgtcg agatagaaga cgggctcccc
     5881 ggtatcgttg tcgaagatga ttcgggagac catctgctgc agctccgggg taaggaagcc
     5941 gggggagagg atgccgaagc gcaggaggag ccgtttcgga atgggcatct cgtggccgga
     6001 cttgttcgcc acgctcgagc ccaggtccca gaacgccgag atgagctgag cccgaaggtg
     6061 ggatttctcc tccgggtctt ccgccttcat gtattttccg aggagctcgt gtacccgggg
     6121 gccggtgtct ccctccccgc tcaaggcctt gcgatagtag tccttgtcct ggaagaacgg
     6181 cttgggatcc ccttcgatct tggtgatggc ggggaagtgg tttcgggtga cctccacctc
     6241 atccggcttg cccctcgccg ggggcttctc ttccccgaag agcgtgtgga agtccggctc
     6301 gccacctttg agatccagcg agggttccgg ctccgacggc gtcttctcgc caccggagtc
     6361 caggtccaga agcgctgcaa gatcgggatc tagactgtcc ggtgcccccg ctgccatcgc
     6421 tgttctatct tatcccggca tatggatttg tcaatgcgcc cgacggcggg cacggcgggc
     6481 gcgcatggag agcaggctgg gacatccggg ggtagccttc cgatccccat ccggcgggtg
     6541 tcctacgtta cgttctg
//
LOCUS       DASRZS010000023         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_781506, whole genome shotgun sequence.
ACCESSION   DASRZS010000023 DASRZS010000000
VERSION     DASRZS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781506"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFH83_00570"
     CDS             complement(<1..574)
                     /locus_tag="VFH83_00570"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HET6484878.1"
                     /translation="MNLRSMLDSLKSRKFRFGGYATLMVIVVLAVLVAVNLLVDQIPG
                     KLDLTQNKIFSLSPETYKVVSGLTNDVTITMLAKPGSEDPTIKAILDKYAAASRHVKI
                     QSIDLELNPTWAKQYETPGTSLSAGGVVVAGAKKFRTIGLYDMYNYDTSNPNQQPQLT
                     SLSVEQRVTSALLYIGSARNVTLTALQGQGE"
     gene            complement(588..1457)
                     /locus_tag="VFH83_00575"
     CDS             complement(588..1457)
                     /locus_tag="VFH83_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6484879.1"
                     /translation="MFAVFRREFKAYFTSPIGFIFVGFFILLAGVFFSLSNLLTGSPA
                     FTGVLSNLTFIFLFLVPILTMRLISEETRQKTDQLLITSPLSLTGIIVGKYLAAVGVF
                     FITLVITVIYPVIMSFYALGGLGWWEILGGYIGFFLLGSSFIAVGLLFSSLTDNQLIA
                     AVETFAALLLIWILDYIGQSVPSDPTSGLVFLALVAAALVLVVFLSTRNTLASVVTFI
                     VLAAVVVLLFVLKKSFFEGLIARALAWFSLLKRYNDFGMGILGLSPIIYYISFSGAFV
                     FLTIRMIEKRRWV"
     gene            complement(1459..2406)
                     /locus_tag="VFH83_00580"
     CDS             complement(1459..2406)
                     /locus_tag="VFH83_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6484880.1"
                     /translation="MIQVENVTKRYGQRLAVDRLNFTVNKGEILGFLGPNGAGKSTTM
                     NIITGYLSATEGSVKIDGHDILEEPREAKKQIGYMPELPPLYVDMTVQDYLSFVCDIK
                     GVPRARRTQNMDRAMQLLMITDVRGRLIKNLSKGYRQRVGIAQSLIGTPPALILDEPT
                     IGLDPQQIIETRNLIRDLGKEHTIVLSSHILPEVQAVCSRVLIINHGKIVASDTPENL
                     SRGLSSENRLTLRVAGKEAQALSVARSVSGVAKVEAVSSREAGTVDLLVEAHKEADVR
                     RDVFKAFAKADLPILMMKSMDLSLEEIFLNLITEEKEVS"
     gene            complement(2494..3249)
                     /locus_tag="VFH83_00585"
     CDS             complement(2494..3249)
                     /locus_tag="VFH83_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HET6484881.1"
                     /translation="MSSPSIRRYRGVGSVRSYAGYLLDADNTIFDYDLCEREALVELL
                     EGVAPGFPAERGLALYREINADYWRRFELGQVGVEDLKRGRFRDLLGALGADGDAEAV
                     SSRYLEALSRRAYFLPHAEAALRDLSRRARLCLVTNGLSSVQRGRLAAAGIEPRFHAV
                     LISEELGFAKPDPRFFQAAVSAVGLPPAELLCVGDSPTSDIAGARDFGMDTCWVSAGS
                     SEWPGPGPEPTYVIPDLRWLTNPGSAVAQGARI"
     gene            3349..3525
                     /locus_tag="VFH83_00590"
     CDS             3349..3525
                     /locus_tag="VFH83_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484882.1"
                     /translation="MAEPLSKNARVGVVTQKFFCTCGGEVKMKLVFNSGKLRNVAECD
                     KCKRQERKPSDFKA"
BASE COUNT          669 a         1117 c         1168 g          622 t
ORIGIN      
        1 cctcgccctg tccctggagg gccgtcaagg tcacgttgcg cgccgatccg atgtacagga
       61 gggccgaggt cacccgctgc tcaacggaca gcgaggtgag ctgcggctgc tggttggggt
      121 tggaggtgtc gtagttgtac atgtcgtaca acccgatggt gcggaacttc ttggcgccgg
      181 ccaccaccac cccgccggcg gacagcgagg taccgggcgt ctcgtactgc ttggcccacg
      241 tggggttcag ctcgaggtcg atgctctgga tcttgacgtg gcggctggcc gctgcgtact
      301 tgtccaggat cgccttgatg gtaggatcct cgcttcccgg cttggccagc atcgtgatgg
      361 tcacgtcgtt ggtgagcccg ctcaccacct tgtaggtctc cggggacagc gagaagatct
      421 tgttctgggt cagatccagc tttccgggga tctggtccac caggaggttg accgccacga
      481 gcaccgccag gacgacgatg accatcaacg tggcgtagcc cccaaagcgg aacttgcggc
      541 tcttcagcga atccaacatc gaacgaaggt tcacggttcc gcctccctta cacccagcgt
      601 cgcttctcga tcatgcggat cgtgagaaac acgaacgcgc cgctgaacga gatgtagtag
      661 atgatggggc tcagacccag gatccccatg ccgaagtcgt tgtaccgctt gaggagggag
      721 aaccacgcca gcgctcgggc gatcagcccc tcgaagaagc tcttcttgag cacgaagagg
      781 agcaccacca cggccgccag cacgatgaac gtcacgactg acgccagggt gttgcgggtg
      841 gacaggaaga ccaccagcac gagcgccgcc gccaccaggg ccaggaacac cagacccgag
      901 gtggggtccg agggcacgct ctgccctatg tagtcgagga tccagatcag gagcagcgcc
      961 gcgaaggtct ccacggccgc tatgagctgg ttgtccgtca gggaggagaa gagcagcccc
     1021 acggcaatga acgaggagcc caggaggaag aacccgatgt atccgcccag gatctcccac
     1081 caacccagcc ccccaagcgc gtagaagctc atgatgaccg ggtagatcac cgtgatcacc
     1141 agggtgatga agaacacccc taccgcggca aggtacttgc ccacgatgat gcccgtgagg
     1201 gagagcgggc tggtgatgag gagctggtcc gtcttctgcc gggtctcctc ggagatcagc
     1261 cgcatggtca ggatcgggac caggaacagg aagatgaagg tcaggttgct gagcacgccc
     1321 gtgaaggcgg ggctccccgt cagcaggttg ctgagggaga agaagacccc ggccagcagg
     1381 atgaagaacc ccacgaagat gaagccgatg ggggaggtga agtatgcctt gaactcgcgg
     1441 cggaacacgg cgaacatgtc aggatacctc cttctcttct gtgatcagat tcaggaagat
     1501 ctcctccagg ctcaggtcca tgctcttcat catgaggatg gggagatccg ccttggcgaa
     1561 tgccttgaac acgtcccgcc ggacatccgc ttccttgtga gcctccacca ggaggtccac
     1621 ggttccggcc tcccggctgg aaacggcctc aaccttggcc acgccgctca cggaccgcgc
     1681 cacggagagc gcctgcgctt ccttgccggc cacgcggagg gtcaggcggt tctcgctgct
     1741 caagccgcgg gagaggttct ccggcgtgtc gctcgccacg atcttcccgt ggttgatgat
     1801 gagaacccgg ctgcagaccg cctggacctc gggcaggatg tgggagctca gcacgatggt
     1861 gtgctccttg cccaggtcac ggatgaggtt gcgggtctcg atgatctgct gggggtccag
     1921 accgatggtg ggctcgtcca ggatgagggc cggcggggtt ccgatgaggg actgggcgat
     1981 ccccacgcgc tggcgatacc ccttggacag gttcttgatg agccggccgc ggacgtcggt
     2041 gatcatcagg agctgcatgg cgcggtccat gttctgggtc cgtctggcgc ggggcacacc
     2101 tttgatgtcg catacgaagc tgaggtaatc ctgtacggtc atgtcaacgt acaggggcgg
     2161 aagctccggc atgtagccga tctgcttctt ggcttcgcga ggctcctcca ggatgtcgtg
     2221 accgtcgatc ttcaccgatc cctccgtggc ggagagatac ccggtgatga tgttcatggt
     2281 cgtcgacttg cccgccccgt tgggtcccag aaaccccagg atctcgccct tgttcacggt
     2341 gaagttcagg cgatccacgg ccagccgctg accgtagcgt tttgtcacgt tttcaacctg
     2401 tatcaaggca gtctcctttc tccaggtaga ttccggcgat gacgacacgg ggtaccgtgt
     2461 ctgagctcct tgttcggtat caagccaaac atatcagatt cgcgcgcctt gggccacagc
     2521 cgaaccgggg ttcgtaagcc agcgcaggtc ggggatgacg tacgtgggct cggggcccgg
     2581 tccgggccac tccgaggacc cggcggacac ccaacaggtg tccatcccga aatcccgcgc
     2641 gccggcgatg tcgctggtgg ggctgtctcc cacgcagagc agctccgcgg gaggcagccc
     2701 gaccgcggat accgcggcct ggaagaaccg cggatccggc ttggcgaacc ccagctcctc
     2761 cgagatgagc acggcatgga agcgcggctc gatcccggcg gccgccagtc ggccacgctg
     2821 cacgctggac aacccgttgg tcaccaggca gaggcgggcc cgccgggaga gatcgcgcag
     2881 cgccgcttcg gcgtgcggaa ggaagtatgc cctccgggag agcgcttcga gatagcggct
     2941 ggaaaccgct tccgcgtccc cgtccgctcc cagggcgccc agcaggtccc ggaagcggcc
     3001 ccgcttcagg tcctccacgc ccacctgacc gagctcgaat ctgcgccagt agtccgcgtt
     3061 gatctcgcgg tagagggcga ggccccgctc cgcggggaag ccgggcgcca ccccctccag
     3121 gagctcgacc agggcctccc gctcgcagag gtcgtaatcg aagatcgtgt tgtccgcgtc
     3181 caggaggtac ccggcgtagc ttctcacgga accgaccccc cggtagcggc ggatactggg
     3241 acttgacatt tctcgcccaa accgtaaatt ctcgtggccg cgcgaggtcc gggttccgcg
     3301 tgctcgcgtc ggtttccccg aaagcttacg gaaggaggtt ctcggaccat ggcagagcca
     3361 ttgagcaaga acgcaagggt cggagtcgtg acgcagaagt ttttctgcac ctgtgggggc
     3421 gaggtcaaga tgaagctcgt cttcaacagc ggcaagctgc ggaacgtcgc cgagtgcgac
     3481 aagtgcaagc gccaggagcg caagcccagc gatttcaagg cctgagcccc ctcatcgcct
     3541 gacccgaagg gcggggctct acgccgtccg aagggc
//
LOCUS       DASRZS010000024        15994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3475371, whole genome shotgun sequence.
ACCESSION   DASRZS010000024 DASRZS010000000
VERSION     DASRZS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 15994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15994
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3475371"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VFH83_00595"
     CDS             <1..491
                     /locus_tag="VFH83_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6484883.1"
                     /translation="YDFSTSRGIVSIIGHTHRPLFESLSMIDTLQFRIEQLCRDYPEV
                     SPLAQREIEAAIEGYRQQLARLWERDRDEGLRGSLYNEQLCIPCLFNSGCAVGKRGVT
                     AIEIADGSILLVHWSEGAPGEDSVGAEEQASQRLGSTDYLRTVLKQDRLDYVFARIKL
                     LT"
     gene            601..2475
                     /locus_tag="VFH83_00600"
     CDS             601..2475
                     /locus_tag="VFH83_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HET6484884.1"
                     /translation="MAQRIAHRVGDATAGDAGETLRIAGRDDPQEEVWAVVRTADPDA
                     RRVATVVCRAVAEAVEGGVRAGELDRPRRSIRLVLDLEPSGYQVWLERSDLFQPPLAG
                     IGLGLVGVRPGQCGHLLSVQGTTGPSAVFVNDLCALVAKGVLAGDNPGYRVRAEPCRF
                     EDAVFWGDPKGGFPCPWLETRTAIREPVPDMVSLSALVSGRALRACATVVGAYLLYLA
                     DAGTAELLDVARSHTADVIRTLEGQRGSQSGAWAELRVVQHHLSLQRLQRWAWGGDHA
                     GILRELAAMEAAVRRAAHARKPIAKVGLVAFRTGRVGRRQSPGLDPRPLFWVNGERTV
                     DQIASLQTADAASTVAEVEQGLQAWEGAGSVQFVSPTELVSAARLKQDLRTLGVRVGM
                     DLMVHSSLSSIGLVRGGAETVVDALLSAVGTRGTLLMPSFNHGHAAIYNPLTTRTTNG
                     AIADAFWRRPRAVRSLNGTHPVAALGPRARGYCEGHVEAGIWTADSPIGRLVQRGGWI
                     LCLGVGLDRATAYHVAEVSVPCRCLDMFAGRDRRVQRDGSVVASPGLLWRGGTCPVPP
                     QRLERGLARLGQLSRGRIGRAPSLLCAAVDLWSLRRRHLAPVCPTCPVRPDPGWKRVR
                     "
     gene            2651..3274
                     /locus_tag="VFH83_00605"
     CDS             2651..3274
                     /locus_tag="VFH83_00605"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET6484885.1"
                     /translation="MPNQTLPTIGDRLSDKGVSWAWHSGGWNDALAGRPDPLFQFHHQ
                     PFIFFAKYADGTEAKRVDLKDEKDFLSAVAEGSLPGVSFVKPLGPDNEHPGYASLFRG
                     QEHVADLVSRIRVSSLWNDSIIIITCDENGGRWDHVSPPVVDRWGPGTRVPTIVISPF
                     ARRATIDHTQYDTTSILKLIETRWDLQPLGSRSRDAAAGNLLNALSF"
     gene            3342..4724
                     /locus_tag="VFH83_00610"
     CDS             3342..4724
                     /locus_tag="VFH83_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484886.1"
                     /translation="MVRFGTAIRDITPPYPVMLHGYAARNRRSGDGPEDRVTEPLSVR
                     ALCVEGLARAGTVRRVVILALDTIGVHAGQIAGLRQAIQRATRVDPSDLLIAATHTHF
                     APAISPQLLHSPELGVVEPDARFVAAVTARVVEAVSESVQAMVEGELEEYRVEVPSVL
                     FNRRTVVKGPASKRVETNFLYPAHPRDFDFSPVDPQLTAFRFRTASGPKAALLTFGCH
                     PVTGGLRGELSHYDISADYPYYVRKTIEDAWGCPVLFCLGAAGDAVPMRRMGRSREHI
                     GATLGSAVLLGERAFASSEPADSAVDTRVLELEAKTIVSTLGSGVEEAYRKARERLLG
                     LQRGAPAASEETVREATEEFLHRARLAYRARLYPEDHLRFQIQLLRLGQSVLVALPFE
                     VLSEISLRIKRAFPNAVVVSVANGYEGYLPLAYEYERGGYEATAESTHVEIGTADRVL
                     ERVLTELATF"
     gene            complement(4764..5393)
                     /locus_tag="VFH83_00615"
     CDS             complement(4764..5393)
                     /locus_tag="VFH83_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HET6484887.1"
                     /translation="MSDIPLLIDEPQGRPSGLTIVLAHGAGAAMDSSFMDEAAAALSA
                     QALRVVRFEFPYMHGARVTGKRGRPDLPHVLEKTWLTVVEQLGDPSNLVIGGKSMGGR
                     IASMVADRAGVRGLVCMGYPFHPAGAPKTLRVAHLKDLRTPTLILQGERDALGSREEI
                     DAYELSSSIRVVFLPDGDHSFKPRKSSGHTYPENMARAMEEAVSFCRRL"
     gene            5425..7464
                     /gene="ligD"
                     /locus_tag="VFH83_00620"
     CDS             5425..7464
                     /gene="ligD"
                     /locus_tag="VFH83_00620"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET6484888.1"
                     /translation="MAMEEYRRKRDFQKTPEPAPLVGADTGNRFVVHRHEARNLHYDL
                     RLEAGGVLACWAVPKGFSYAPTDKRLAVRTEDHPLMYQEFEGTIPKGQYGAGTMRIWD
                     TGTFEIRKAAGIAQALAKGELKLEMKGSRLRGEWHLVQTKQEAGKNWLLFKAKDRYAG
                     SGSDLFGGADVSRAARRPPPRSVARMEATAGERAFSDPDWLFEPDLIGRRVLVRVQEP
                     DVSLRAGSVDLAPQLKDIVSALGFLRSRTAVLDGIVVALDEKGRPSQEALASELASGG
                     SRTVLYLFDVLYAEEWDLRKCPLSERKAVLRALLPDSNRLFSVDPVPERGEMLAHAAA
                     EAGLPSILGKRSGSTYASGESSEWLRIPASLSPARPPGNLGPAARAKATKEKRPAFQV
                     THPRKVYWPDQGYTKGDLVGYYDQVAGWILPYLKDRPLHLYRWPDGIRGKSFYQKQLP
                     EDLLEGVETVDVARDGEPPTLYAMCNDRATLLTLINSGSIDLHPWMSRKGSLDSPDWS
                     ILDLDPKTASFSDVIKVARVVGRILRGVGIEPYLKTSGSSGLHVCIPLAPGYSYEQSR
                     MFCEAIARLVVRDHPDLATVERVVSRREGRVYIDFLQNRREQTVVPPYVVRPVEAASV
                     SMPLAWDELEGEFRIADFTLMNAPRRIERTGDLFRTALSRPQDLAPAIDALGKYL"
     gene            7477..10914
                     /locus_tag="VFH83_00625"
     CDS             7477..10914
                     /locus_tag="VFH83_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012480249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HET6484889.1"
                     /translation="MYLEGGQFRLAATDVSNHLACRHLTQLDRAVAEGRLSAPAYRDP
                     NLAVLQQRGFLHERAYVAYLKAEGLRVAEPPEAGGRLSMERALQAMRSGADAIVQAEL
                     RDGRWIGRADVLLRVPQPGAFGPWSYEVVDTKLAEETRAGTVLQLCHYSELLATVQGV
                     APEWMHVVKPGPDFPRESFRYDEVSAYYRLVRRRLDHVLSEPPTPSTYPYPVPHCEIC
                     RWWKACDERRHADDNLCLVAGIQRLQTAELERQGIGTVEQFAREPKATREKPRRGSPE
                     SFVRIHGQARVQLEGREAGKPRYRILRPEPGLGFYQLPAPDAGDVFFDIEADPFAGDG
                     GMEYLLGFAFLRDGGMEYQGLWALDRVAERTALERFMDFIRERWQTNPGMHIYHYSPY
                     EPAAVKRLAGRHGTREAELDRLLRAERFVDLLAVTRRAVQASVESYSLKALEPFDRFA
                     RSVELPQAAAALRRIARSLELTGPADITAADRAATEAYNRDDCLSTAALRDWLERLRK
                     GLEVLVPRPESPSGDAPEAVEERAAEVEEVYHQLVDALPEDRSAWGSEARARWLLAHQ
                     LEYFRREDKSAWWEFFRINELDPEELLDERKAVAGLRFVGPIGRAGKMPIHRYEFPDQ
                     EVAMDEGDELYEVGGETIGKVHAIDLSRHTLDIVKKGRTIQTHPSAVMVDEHVRPAPV
                     DGSFLQLARSVAEKGVDGSGPYRAARDLLLADPPRLRRSGGQRAEPEAGSSTPGPLRA
                     PGERVVEAAVRLVKALDGGVLPIQGPPGSGKTYTGARMIVALARDGKRIGVTAVSHKV
                     IQNLLAEVRRAAAQEGGDIRLMHKEGRSQTSETEGIELAPDNETARAGLLAGKVVGGT
                     AWLWSRDDMEGLADYLFIDEAGQMSLAHALATARSARNLVLLGDPQQLEQPQRGAHPE
                     GADVSALVHVLGGRKTIPDEAGLFLDETWRLHPGICRFTSEVFYERRLQTRPGLERQG
                     LTGDTPVSGAGLFYVPVEHAGNQNRSMEEVDAIDRLVSSLLGGGVRWTNARGEERPLC
                     DADILVVAPYNAQVTSLINRLPRGVRVGTVDRFQGQEAPVVIYSMTSSSAQDAPRGMS
                     FLFSPNRLNVATSRARCVCILVAAPGLLEPECSSPEQMIWANALCRYRELATEVAVL"
     gene            complement(10911..12104)
                     /locus_tag="VFH83_00630"
     CDS             complement(10911..12104)
                     /locus_tag="VFH83_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6484890.1"
                     /translation="MPTETPLAGKEIALTGRLFSMTREEAIERIRNAGGLYVRSPGQT
                     TAFLVAGSALGHLTPEGILPRRMGQFRELKSRGASIRLMDEPELLRLLGADEDLEDFS
                     RLYTGEQVSRIVEVPATEVRAWVRRGLLRPARLTHRLAWFGFEDILTARNLSRLTASG
                     VPASLIHQSLSQIAQWLPDGERILERLEAYATGLRLRLPDGSWVEPSGQRLMDFQIGG
                     HAAAVSPPTARITQFPSDGGVERHPAEETGDWFSLAVEAEESGNLEAAANRYTRALET
                     ATGPEISFNLGNVLYELGREADAAERYLQALDADRNFAEAWNNLGNSLVALGRLGDGV
                     QAYEMALSLEPEYPEPHCNLTAVLDRMGQFEKAHAHRAICQRAFPSPVRLRILRQSSL
                     EGSES"
     gene            complement(12109..13005)
                     /locus_tag="VFH83_00635"
     CDS             complement(12109..13005)
                     /locus_tag="VFH83_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484891.1"
                     /translation="MSRELPRFVPPMLAIQGQPFDSELHFFEFKWDGFRACAMIENGS
                     VRLMSRNGIDLAPQFPGLSGLGKLPGGLALDGELVAFRDGRPVFDLMLNRGRGAGSKA
                     VRFVVFDVLYTDFESRMKLPLLDRRPILEEVVARQPCPELMLSEGVPANGKALYQKAC
                     EQGLEGVMAKRLSSTYAAGKRNGAWVKIKRRLHIQVAVIGFIEKASNDFQSLLVAGSS
                     LPGEEPGPLRYLGRVGGGFTETQHAHVNDLLRRRPRVAPLVPCPEKGRWVEPGLYCEV
                     SYAELTEAGLLRAPVFEGLIEE"
     gene            complement(12998..13930)
                     /locus_tag="VFH83_00640"
     CDS             complement(12998..13930)
                     /locus_tag="VFH83_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET6484892.1"
                     /translation="MAAHASWKGFLKLSLVSVPVKAYTATATGNDITLHQLHAKTHTR
                     IQYKKFAPEVGEVSNEEIVKGYEYDKGQYVIVDEEELDKLRTESDKSVRIDGFIKPEE
                     VSSIYLGGRTYFLTPDGPVGQKPYTLLRKGMEANGVCAIAQVVISQKEQVVLLRPLDG
                     LLAMTVLSRKDEVKSASAFKDEVTETEVTEAETSLTDTLIKASMIKAFDFARYKDVYK
                     EKLTQLIQLKIEGKEVIQVRDPEEPKIINLMDALKRSVAEAQAAGGSVSAEEEGEKSK
                     MKADLKQAPSAKPAKTVGTTAGAATATAKKKKVV"
     gene            complement(14072..14578)
                     /locus_tag="VFH83_00645"
     CDS             complement(14072..14578)
                     /locus_tag="VFH83_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6484893.1"
                     /translation="MQETVQQYVSRILSYLEDQKPVDLLVSSPRKISALVKGATKRQM
                     GKRPAPGKWSVTEILAHLADVEIVQGFRVRLMLASNGVPIQGFDQDTWAGEFGYAKQD
                     PAASLKAYLVNRERNVRLMKSLTPEQWSRYGIHSERGKETVFRVAELMAGHDINHLRQ
                     IRSNLKGK"
     gene            complement(14673..15176)
                     /locus_tag="VFH83_00650"
     CDS             complement(14673..15176)
                     /locus_tag="VFH83_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6484894.1"
                     /translation="MQVMAQDPTNGILDFPKALGRWASETQGAPGEEARAGITDRFLR
                     SLLERWGSRDRPPSAGPTEATLAAERIISDRSLRTVADAARALGLDVRGLQRLFQREV
                     GIGPKELILRFRLQEAAERLLRQPDLRSSDLALDLGYFDQAHFIRDFKSVTGIPPEAY
                     QRRQPQR"
     gene            15297..15734
                     /locus_tag="VFH83_00655"
     CDS             15297..15734
                     /locus_tag="VFH83_00655"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6484895.1"
                     /translation="MAAETFSLSVDEVIDARPAQVWKVLTDPVHVGELFWGSTVESDF
                     TPGHPIVWKGVWQGKPFEDRGSIRRAEENVLLQVTHWSPSSGPETEDNHTLLTWRIRP
                     DGGKVRVTLDHENIRTRETRDHSEGMWKQLLGRMKEMLEKPPD"
     gene            15771..>15994
                     /locus_tag="VFH83_00660"
     CDS             15771..>15994
                     /locus_tag="VFH83_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484896.1"
                     /translation="MSAPPTGSQPIGPYPQNPHYFSYKGSPVLLITSDQHYGAVVNAD
                     FDYRAFLDAIASYGMNFTRIYGGAYIERDG"
BASE COUNT         2642 a         5201 c         5398 g         2753 t
ORIGIN      
        1 tgtacgactt ctccacgagc cgcgggatcg tgtcgatcat cggacatacc catcgacccc
       61 tcttcgaatc cctttccatg atcgatacgc tccagttccg catcgagcag ctctgccggg
      121 actaccccga ggtctcgcct ctggcccaga gggagatcga ggcggccatc gagggatatc
      181 gacagcagct tgcccgcctg tgggagcggg accgggacga gggccttcgc gggagcctct
      241 acaacgagca gctctgcatc ccctgtctgt tcaactccgg ctgtgccgtg ggcaagcgcg
      301 gagtaacggc catcgagatc gccgacggtt ccattctgct ggtgcactgg tctgaagggg
      361 ctccggggga agattccgtc ggcgcggaag agcaggcttc ccaacggctg ggctcgacgg
      421 actaccttcg caccgtgctc aagcaggatc gactcgacta cgtctttgcc aggatcaagc
      481 tcctgacctg agctgcaggc aatcgggaac cggaggacta tcgtgcacac gccgcttacc
      541 ctgcgccgga gcaaccggcg gtccctgcca gccctccggt ctcggctgga cggtcgcgcc
      601 atggcgcaac ggattgcgca ccgtgtcggc gatgcgacgg ccggggacgc cggagagacg
      661 ctgcgcatcg ccggaagaga cgacccccag gaggaagtgt gggccgtcgt ccggacagcc
      721 gatccggacg ctcggcgggt ggccaccgtg gtatgccgcg ccgttgcgga ggcggtggag
      781 ggcggcgtgc gggcaggaga gctggacagg ccgcggcgct ccatccgtct ggtcctggac
      841 ctcgagccct cgggatacca ggtatggctc gagcgttcgg acctctttca gccgccgctt
      901 gccggcatcg gccttggcct ggtaggggtg cgcccgggcc agtgcggcca tctcctcagc
      961 gtgcagggga cgaccgggcc atcggcggta ttcgtgaacg acctttgcgc cctggtagcg
     1021 aaaggcgtcc tggccggcga caaccctggg tatcgggtcc gagccgagcc gtgtcggttc
     1081 gaggacgccg tgttttgggg cgaccccaag ggtgggtttc cgtgcccgtg gctggagacg
     1141 cggaccgcga tccgcgagcc cgtgcccgat atggtgagtc tttccgccct cgtgagcggc
     1201 cgggcgctcc gtgcctgcgc caccgtggtc ggcgcgtatc tgctctacct ggcggacgcc
     1261 gggaccgccg agctgctcga tgttgcccgc tcgcacaccg cggacgtgat ccggaccctg
     1321 gagggccaac gaggcagcca gtcaggcgcg tgggcagagc ttcgcgtagt gcaacaccac
     1381 ctctcgttgc agcggctcca acgctgggca tggggagggg accacgccgg gatcctgaga
     1441 gagctcgccg ccatggaggc agcggtccga cgggcagctc acgcacgaaa gcctattgcc
     1501 aaggttggcc tggtcgcgtt ccgcaccggg cgggtcgggc ggcgccagag cccgggtctc
     1561 gacccgcgcc ccctcttctg ggtgaacgga gagcggacgg tcgaccagat cgcgtctctc
     1621 cagaccgccg acgccgcaag cactgtcgcg gaagtcgagc aagggctgca ggcctgggaa
     1681 ggagccggct ctgtgcagtt tgtctctccc acggagctgg tatccgccgc ccggctgaag
     1741 caggaccttc ggacactcgg ggtgagggtc ggaatggacc tcatggtcca tagctcgctc
     1801 tcgagcattg gcctggtgcg cggcggagcc gagaccgtgg tggacgctct cctgtccgcg
     1861 gtggggaccc ggggcaccct gctgatgccc tccttcaacc atggccacgc ggcgatctac
     1921 aaccctctga ccacgcgcac gaccaacggc gccattgccg acgccttctg gagacggcct
     1981 cgagcggtgc ggagcctgaa cgggacgcat ccggttgccg ctcttggacc gcgggcccga
     2041 gggtattgcg aaggccacgt cgaggcgggg atctggaccg cggacagtcc catcggacgc
     2101 cttgtacagc gcggcggctg gatcctgtgc ctgggggtcg ggctggatcg ggccaccgcc
     2161 tatcacgtgg cggaggtgtc cgtgccatgc cggtgtctcg acatgttcgc gggacgcgac
     2221 cggcgcgtgc agcgggacgg atcggtcgtg gcgtcaccgg ggttgctgtg gcgcggaggg
     2281 acctgtccgg ttccccctca gaggctcgag cgtgggctgg ccaggctggg ccagctatcc
     2341 cgaggcagga tcgggagagc cccatcgctc ctgtgcgccg cggtggatct ctggagcctg
     2401 cgtcgaaggc accttgcacc cgtgtgcccc acctgcccgg tgcgacccga tccggggtgg
     2461 aagcgggtgc gttagcggca gcccgcgccg ttccctacgg gaagaaaatc accggttctt
     2521 caccgatccc taaccctccc ccaacggatc atcgtgtcct tctggcacgt tgacctcatg
     2581 ccgaaaggag cagcaatgaa agcgactcgt aaacgctccc gtcccgccgg gcgccaaccc
     2641 ggcgaccctg gtgcccaacc agacgctgcc tacgatcggg gaccggctct cggacaaggg
     2701 cgtaagctgg gcctggcact cgggagggtg gaacgacgcc ctggccggaa ggcccgatcc
     2761 cctgttccag ttccatcatc agccgttcat cttctttgca aagtatgccg acggtacgga
     2821 ggcgaagcgc gtcgatctga aagacgagaa ggactttctg tccgcggtgg ccgaaggctc
     2881 gctccctggc gtgtcgttcg tgaaaccgtt gggcccggac aacgagcatc cgggatatgc
     2941 gagcctcttt cgggggcaag agcatgtggc agacctggtg agccggatcc gggtcagcag
     3001 cctgtggaac gactcgatca tcatcatcac ctgcgacgag aatggaggcc gctgggacca
     3061 cgtgagcccg cccgtcgtgg accgctgggg ccctggaacg cgggtgccaa ccatcgtgat
     3121 ctcgccattc gcccggcggg ccaccatcga tcacactcag tacgacacca cgtccatcct
     3181 caagctgatc gagacgcgct gggacctcca gcccctgggc agccgcagcc gggatgcggc
     3241 agcggggaac cttctgaacg cactgtcctt ctgaggcgga ttccgggcca gccgacggac
     3301 ggggtccttc tgctggcctt ctccatggta cagtcccggt catggttcga ttcgggacag
     3361 ctatccggga cattacgccg ccctaccccg tgatgctgca cgggtatgcg gcgcggaatc
     3421 gacggtcggg ggatggaccc gaggatcggg tcaccgagcc tctgagcgtg agggcgttgt
     3481 gtgtcgaagg tctcgctcgg gcgggcacgg tgaggcgggt cgtcattctc gcgctcgaca
     3541 ccatcggggt ccacgccggt cagatcgccg ggttgcgaca ggcaatccaa cgagccacgc
     3601 gggtcgatcc atcggatctc ctgatcgcgg ccacgcacac gcacttcgct ccagccatca
     3661 gtccgcagct cctccacagc cctgagctcg gggttgtcga gccggatgcc cggttcgtag
     3721 ccgcggtaac ggcacgggtg gtcgaggccg tgtcggaaag cgttcaagcg atggtcgaag
     3781 gagagctgga ggagtatcgc gtagaggtcc cctcggtgct gttcaaccgc cgaacggtgg
     3841 tgaaggggcc agcctcgaag agggtcgaga ccaactttct ctatcccgcg catccgcggg
     3901 acttcgattt ctccccggtc gacccgcagc tcacggcgtt tcgcttcagg accgccagcg
     3961 gccccaaggc ggcgctcctc acctttggct gtcatccggt cacgggcggg ttgaggggcg
     4021 agctcagtca ctacgacatc tctgccgact acccctacta cgtgcgaaag acgatcgagg
     4081 acgcctgggg ctgccccgtg ctcttctgtc tcggggcggc cggggacgct gtcccgatga
     4141 ggaggatggg ccgttcgcgg gagcacatcg gcgcgacgct cggcagcgcg gtccttctag
     4201 gggagcgcgc gttcgcttcc tccgagccgg cggactccgc agtcgacact cgggtgctgg
     4261 agttggaggc gaagaccatc gtttcgacgc tgggaagcgg agttgaggag gcctaccgga
     4321 aggcgcggga gaggctgctg gggctgcagc gcggcgctcc ggcggcgtcg gaggaaacgg
     4381 tgagggaggc caccgaggag ttcctgcaca gggctcggct tgcataccgg gcgaggctct
     4441 atcccgagga tcatctccgg ttccagatcc agctcctgcg tcttgggcaa tccgttctcg
     4501 tggccttgcc gttcgaggtg ctctccgaaa tcagccttcg catcaagcgc gcgttcccga
     4561 acgccgtcgt ggtctccgtg gccaacggct acgaggggta cctgccgctg gcgtacgagt
     4621 acgagcgggg cgggtacgag gcaacggcgg agtccacgca cgtcgagatc gggaccgcgg
     4681 accgggtcct ggaacgggtg ctgacggagc ttgctacctt ctagaaaacg ggttggaaga
     4741 gcggaacccc gcggcccgtc gcgtcacagc ctgcggcaga agctgacggc ctcctccatc
     4801 gctcgggcca tgttctccgg gtaagtgtgc cctgagcttt ttctcggctt gaacgagtga
     4861 tccccatccg gcaggaacac aacgcggatc gacgaggaaa gctcgtaggc gtcgatctct
     4921 tcgcggctcc ccagagcatc ccgttctcct tgcaggatca gggtgggggt tcgcaggtcc
     4981 ttcaagtgag ccactcgaag cgtcttcggg gcacctgccg ggtgaaacgg atatcccatg
     5041 cacaccaggc cccgaactcc agcgcggtcc gcgaccatgc tggcgatccg tccccccatg
     5101 gattttccgc cgatgaccag gttcgacggg tctccgagtt gttccaccac cgtgagccac
     5161 gtcttctcca ggacgtgggg gaggtccggg cgaccgcgct tcccggtgac ccgggcccca
     5221 tgcatgtagg ggaactcgaa gcgcaccacg cgaagcgcct gcgcggagag cgcggcggca
     5281 gcctcatcca tgaacgagga gtccattgcg gcgccggccc catgcgccag gacgatggta
     5341 agcccggaag gccgaccctg cggctcgtcg atcaaaaggg ggatatcgct cacgaggcag
     5401 cctcccgttg atacattggg cagtatggcg atggaggagt accgccgcaa gcgagacttc
     5461 cagaagaccc ccgagcccgc gcccctggtc ggtgcggaca ccggtaaccg attcgtggtc
     5521 catcggcacg aggcccgcaa cctgcactat gacctgcgcc tggaggcggg cggcgtgctg
     5581 gcctgctggg cggttcccaa gggcttctcg tatgcgccca cggacaaacg gttggcggtc
     5641 cggaccgagg atcatcccct gatgtaccag gagttcgagg gaaccattcc caaggggcag
     5701 tatggcgccg gtaccatgag gatctgggac accggcacct tcgagatccg caaggcggcc
     5761 ggcatagcgc aggcgctcgc caagggagag ctgaagctgg agatgaaggg gtcccggctt
     5821 cggggggaat ggcacctcgt tcagacgaaa caggaagcgg ggaagaactg gcttctcttc
     5881 aaggccaagg accgctatgc cggatccggg agcgacctct tcggcggggc ggacgtgagc
     5941 cgggccgcgc gtcgaccgcc gccccggagc gtggcacgga tggaggcgac cgccggggag
     6001 cgcgccttca gtgacccgga ctggctcttc gagccggacc tcatcggacg gcgggtgctg
     6061 gtccgggtgc aggagccgga cgtgagcctg agggcaggtt cggtcgacct ggcgccccag
     6121 ctcaaagaca tcgtctcggc gcttggcttc cttcgctccc ggactgcggt cctggacggg
     6181 atcgtggtgg cgctggacga gaagggcaga ccgtcccaag aagctctggc ctcggagttg
     6241 gcatccggcg ggagccggac ggtgctctat ctgttcgacg tcctttacgc ggaggaatgg
     6301 gacctccgca agtgtccgct cagcgagcgc aaggcggtgc ttcgggccct tcttccggat
     6361 tccaaccggc tcttctcggt ggatccggtc cccgagcggg gagaaatgtt ggcccatgcc
     6421 gccgcggagg ccggcctacc ctccatcctt ggcaaacgat cgggcagcac ctatgcatcc
     6481 ggcgaatcgt cggagtggct gaggattccc gcttctctct ctcccgcacg gcccccgggc
     6541 aacctgggac cggccgcccg ggccaaggca acgaaagaga agcggccggc gttccaggtg
     6601 acgcacccgc gaaaggtcta ctggcccgac cagggttaca ccaagggtga cctggtgggc
     6661 tactacgacc aggtggcggg ctggatcctc ccctatctca aggaccggcc ccttcacctg
     6721 taccggtggc cggacgggat tcggggcaag tctttttacc agaagcagct tcccgaggat
     6781 ctgctcgagg gcgtggagac cgtggatgtg gccagggacg gagagccgcc gacgctctac
     6841 gcgatgtgca acgatcgggc gaccctgctc acgctgatca actccggcag catcgatctc
     6901 catccctgga tgtccagaaa ggggagcctc gactcgccgg actggtccat cctggatctg
     6961 gaccccaaga cggcgtcctt ttccgacgtg atcaaggttg cccgcgtggt tggcaggatc
     7021 cttcgggggg tcggcatcga accctatctc aagacgtccg gctcctcggg actccacgtg
     7081 tgcatccccc tggcgcccgg ctattcttac gagcagagcc ggatgttctg cgaagcgatt
     7141 gcccgcctgg tggtgcgcga ccatccggac ctcgcaaccg tggagcgcgt ggtgagccgg
     7201 cgtgagggcc gcgtttacat cgacttcctg cagaatcgcc gggagcagac ggtggtgcct
     7261 ccgtacgtgg tgcgtcccgt cgaggcggct tccgtctcca tgccactggc ctgggacgag
     7321 ctggaagggg agttccggat cgcggacttc accctgatga acgctcctcg ccgcatcgag
     7381 cgcaccggtg atctcttccg aacggccctg agccgccccc aggatctggc gcccgccatc
     7441 gacgcgctcg gcaaatacct gtaacctggg cccggcatgt acctcgaggg aggccagttt
     7501 cgactggctg ccacggatgt gtccaaccac ctcgcctgcc ggcacctcac gcagctggac
     7561 cgggcggtgg cggagggccg gctctccgct cccgcatacc gggatccgaa tctcgcggtg
     7621 ctccagcagc gaggcttcct ccatgagaga gcgtacgtcg cgtatctgaa ggccgaaggc
     7681 ctccgggtgg ccgagccgcc ggaagccggc ggaaggctct ccatggagcg agctttgcag
     7741 gccatgcgct cgggcgcgga tgcgatcgtc caggcggagc tccgagacgg gcggtggatc
     7801 ggccgcgccg atgttctgct tcgcgtacct cagcccggcg cctttgggcc gtggtcgtac
     7861 gaagtcgtgg acaccaagct tgccgaggag acccgcgcgg ggaccgtcct gcagctctgt
     7921 cactacagcg agctgctggc cacggtgcag ggcgtcgcgc ctgagtggat gcacgtcgtg
     7981 aaaccgggac ccgacttccc ccgggagagc ttccgttacg acgaggtgtc cgcgtactac
     8041 cggctggtgc ggcgccggct cgaccacgtg ctatcggagc cgcccacgcc atccacctac
     8101 ccgtacccgg taccccattg cgagatctgc cggtggtgga aggcgtgcga cgaacgccga
     8161 cacgcggatg acaacctgtg tctggtggca gggatccagc gtcttcagac ggctgagctc
     8221 gaacgacagg gaatagggac ggtggagcag ttcgcgcggg aaccgaaggc cacgcgggag
     8281 aagccgcggc ggggcagccc ggaatccttt gtccgtatcc atgggcaggc cagggtccag
     8341 ctcgagggcc gagaggcggg caagcctcgt taccggatcc tccggccgga gccggggctc
     8401 gggttctacc agcttcccgc gccggacgct ggagatgtct tcttcgacat cgaggccgat
     8461 ccctttgccg gggatggcgg catggagtat ctcctgggct tcgcctttct ccgggatggt
     8521 gggatggaat accagggcct ctgggctctg gaccgcgtcg ccgaacggac cgccctggag
     8581 cggttcatgg acttcatccg ggagcgctgg cagaccaacc ccgggatgca catctatcac
     8641 tattcgccgt acgagccggc cgcggtgaag cggctcgcgg ggcgccatgg cacgcgagag
     8701 gcggagctgg accggctcct cagggccgaa cggttcgtcg atcttcttgc cgtaacccga
     8761 cgcgcggtgc aggcgagcgt ggaaagctac tcgctcaagg ccctcgagcc gttcgaccgg
     8821 tttgcccgca gcgtggagct gcctcaagcg gcggcggcgc ttcgccggat tgcccggtcg
     8881 ctggagctga ccggcccggc tgacatcacg gcggcggatc gcgccgcaac cgaggcatac
     8941 aaccgggatg actgtctctc caccgcggcg cttcgagact ggctggaacg actgaggaag
     9001 gggttggaag tcctggttcc tcgtccggag agcccgtccg gcgatgcgcc cgaagccgtg
     9061 gaagaacggg ccgccgaggt ggaggaggtc taccaccagc tcgtggacgc gcttcccgag
     9121 gaccggtcgg cgtggggatc agaggcgcgc gcgcggtggc tcctggctca tcaactggag
     9181 tacttccgac gggaggacaa gagcgcctgg tgggagttct tccggatcaa cgagctggac
     9241 cccgaggagc tcctggacga gcgcaaggcc gtggcgggtc ttcggttcgt gggcccgatc
     9301 gggcgcgccg ggaagatgcc catccatcgg tatgagttcc ccgaccagga agtggccatg
     9361 gacgagggcg atgagctcta cgaggtgggt ggcgaaacga tcggcaaggt gcacgccatc
     9421 gatctctcgc ggcacacgct ggacatcgtc aagaaaggcc gcaccatcca gacccatccg
     9481 tccgccgtca tggtcgacga gcacgtgcga ccggctccgg tggacggctc ctttttgcag
     9541 ctcgcccggt ccgtcgcgga gaaaggggtg gatggctccg gcccgtatcg ggcggcccga
     9601 gatctcctgc tggccgatcc tccgcgtctc aggcgatctg gaggtcagag agcggagccc
     9661 gaggccggct catcgacgcc cgggcctttg cgggctccgg gagaacgggt cgtggaggcg
     9721 gccgttcgcc tggtgaaggc gttggatggt ggcgtcctcc ccatccaggg gcctccaggg
     9781 tccggcaaga cctatacggg cgcccggatg atcgttgcgc tggctcgcga tggtaagagg
     9841 atcggcgtga ccgccgtgag tcacaaggtg atccagaacc tgctcgcgga ggtccgccgc
     9901 gccgcggcgc aggagggggg cgatatccgg ttgatgcaca aggagggtag atcgcagacg
     9961 tcggagacgg aggggatcga gctcgcgccg gacaacgaga ccgctcgggc agggctactg
    10021 gcgggcaagg tggtcggagg gaccgcctgg ctgtggtctc gggacgacat ggaagggctg
    10081 gccgattatc tcttcatcga cgaggccggc cagatgtccc tggctcacgc cttggccacc
    10141 gcgcgctcgg cccgtaacct cgtcctcctg ggtgacccac aacagcttga gcagccgcag
    10201 cgtggcgccc atccggaggg cgccgacgtt tccgctctgg tgcacgtcct gggcggccga
    10261 aagaccatcc cggacgaagc cgggcttttc ctcgacgaga cgtggcgcct gcatcccggg
    10321 atttgcagat tcacctccga ggtcttctat gagcgacggc tccagacgcg ccccggcctg
    10381 gagcggcagg gcctgaccgg ggacactccg gtttcaggag ccggtctgtt ctacgtcccg
    10441 gtagaacacg cggggaatca gaaccgctcc atggaggaag tggatgcaat cgatcggctc
    10501 gtctccagcc tgcttggcgg tggcgtacga tggaccaatg ctcgaggcga ggagcggccg
    10561 ctctgcgacg cggacatcct ggtcgtggcc ccctacaacg cccaggtgac gtcgctcatc
    10621 aaccgtctgc cgcgcggcgt ccgggttgga accgtggacc ggttccaggg gcaggaagca
    10681 cccgttgtga tctactctat gaccagctcg tccgcccagg atgccccgcg cggcatgagc
    10741 ttcctgttca gtcccaaccg gctcaacgtg gccaccagcc gggccaggtg tgtctgcatc
    10801 ctggtggctg ctcccgggct cctggagccg gagtgcagca gtccggaaca gatgatctgg
    10861 gccaacgcgc tttgccgcta ccgggagctg gcaaccgagg tggcggtgct ctaactttcc
    10921 gatccttcca gggacgactg tcgaaggatg cgcagccgga ctgggctggg gaaggcgcgc
    10981 tggcagatgg cccgatgagc atgcgccttc tcgaactgac ccatgcggtc caggaccgcg
    11041 gtcaggttgc agtgcggctc ggggtactcc ggctccaggg acagcgccat ctcgtatgcc
    11101 tggacgccgt ccccgagcct gcccaacgcc accaaggagt tgcccaggtt gttccatgcc
    11161 tccgcgaagt tgcggtccgc atcgagggcc tgcagatagc gctccgccgc gtcggcttcc
    11221 ctgcccagtt cgtagaggac atttccgaga ttgaacgaga tctccggtcc ggtcgccgtt
    11281 tccagcgccc gggtataccg attcgcggca gcttcgaggt tacccgactc ttccgcttcc
    11341 acggccaggg agaaccagtc tcctgtctcc tccgcagggt ggcgctctac gccgccatcc
    11401 gacggaaact gcgtgatccg cgcggtgggc gggctgacgg ccgcggcgtg gcctccgatc
    11461 tggaagtcca tgaggcgctg cccgctgggt tccacccagc tcccgtccgg gagccgaaga
    11521 cgcagccccg tcgcataggc ctccagccgc tcgaggatcc gctcgccgtc cggcagccat
    11581 tgcgcgatct gggagagact ctggtggatg agagaggccg ggacgccgga cgcggtgagc
    11641 cggctcaggt tccgcgccgt caggatgtcc tcaaacccga accaggcaag gcggtgggtg
    11701 aggcgggcgg ggcgaagaag accgcggcgg acccacgcgc gcacctcggt ggccggcacc
    11761 tcgacgatcc ggctcacctg ctcgcccgtg tacaatcggg agaagtcctc caggtcctca
    11821 tccgcgccga ggagtcgcaa gagctccggc tcgtccatga gccggatcga agccccgcgg
    11881 cttttgagct cccggaactg gcccatgcgc cgcggaagga tgccctccgg cgtgaggtgg
    11941 cccagggcgc tgccggctac gagaaaggca gtggtctgac cgggagagcg aacgtaaagg
    12001 ccgccggcgt ttcggattcg ctcgatggcc tcttcccggg tcatggagaa cagacggccg
    12061 gtgagggcta tctccttccc ggccaggggc gtttccgtgg gcatgaggtc actcttcgat
    12121 cagaccttcg aacaccgggg cgcggagcag tcccgcctcc gtgagctccg cgtagcttac
    12181 ctcgcagtac aggcccggct caacccaccg gcccttctca gggcagggga ccagcggcgc
    12241 gacgcgcggc cgcctccgca agagatcgtt cacgtgagcg tgctgggtct cggtgaaccc
    12301 gcctccgacg cgccccaggt atcggagcgg tccaggctcc tccccaggca gggagctgcc
    12361 ggccaccaga aggctctgga agtcgttgga ggctttctcg atgaagccga tgaccgccac
    12421 ctggatatgc aaccgccgct tgatcttgac ccaggcgccg ttgcgcttgc cggcggcata
    12481 cgtgctggag aggcgcttgg ccatcacccc ctccaggccc tgctcgcaag ccttctggta
    12541 cagggccttt ccgttggcgg gaacgccctc ggaaagcatc agctccgggc acggctgacg
    12601 cgccacgacc tcctcaagga tcggtcgtcg atccaagagg ggaagcttca ttcgggattc
    12661 gaaatccgta tacaggacgt caaaaaccac gaaccgcacg gccttggatc cggcgccgcg
    12721 gcctcggttc agcatgaggt cgaagacggg acgtccatcg cggaacgcca ccagctcccc
    12781 gtccagcgca aggccgccag gaagctttcc gagccccgag aggcccggga actgaggcgc
    12841 aaggtcgatc ccgttgcggc tcatgaggcg gacggagccg ttctctatca tggcacaggc
    12901 cctgaagccg tcccacttga actcgaagaa atgcagctcg gaatcgaagg gctggccctg
    12961 gatggccagc atggggggga cgaagcgagg cagttcccta gacaaccttc ttcttctttg
    13021 cggttgccgt ggcggcgccg gcggtcgtcc ccacggtctt ggccggcttg gcgctcggag
    13081 cctgcttgag gtccgctttc atcttgctct tctcgccctc ttcctcagcg gacacggagc
    13141 cgccggccgc ctgggcctcc gcaacgctgc gcttgagcgc gtccatgagg ttgatgatct
    13201 tgggctcctc agggtcacgg acctggatga cctctttgcc ctcgattttc agctggatga
    13261 gctgggtgag cttctccttg tagacgtcct tgtaacgggc gaaatcgaac gccttgatca
    13321 tggaggcctt gatcagggtg tccgtgaggg acgtctccgc ctcggtaacc tcggtctcgg
    13381 tcacctcgtc tttgaacgcg gacgccgact tcacctcgtc tttgcggctg agcaccgtca
    13441 tggcaaggag gccatccaga ggcctgagca ggactacctg ttccttctgc gagatcacca
    13501 cctgggcgat ggcgcacacc ccgttcgcct ccatgccctt tcgcaggagc gtgtaaggct
    13561 tttgacccac gggaccatcc ggcgtgagga agtaggtgcg acccccaagg tagatggagc
    13621 tcacctcttc gggtttgatg aagccatcga tccggaccga cttgtcgctc tccgtgcgga
    13681 gcttgtccag ctcctcctcg tcaacgatca cgtactgacc cttgtcgtat tcgtaaccct
    13741 tgacgatctc ttcgttgctg acctcgccga cctccggggc gaacttcttg tactggatac
    13801 gggtgtgggt cttggcgtgg agctggtgca gcgtgatgtc gttgccggtg gcggtcgcgg
    13861 tatacgcctt caccggaacc gacaccaagc tcagcttgag aaaacccttc caggacgcat
    13921 gggctgccat cgtttccccc tgaagcgcat cgcgccctga gcgccaggca ccgcccagac
    13981 cgccgctgcc cccctcccgc cccagaagct acaaaggggg tcggacggag tcaaggaaag
    14041 ggtaacccag gcccggggga ttccagcccg gctacttccc cttcaggttg ctgcggatct
    14101 gccggagatg gttgatgtcg tggccggcca tgagctccgc aacccggaaa acggtttcct
    14161 ttccgcgctc cgagtggatc ccgtagcggc tccactgctc gggggtcaag ctcttcatca
    14221 gtcggacgtt ccgctcccgg ttgaccaggt aggctttgag ggaggcggcg gggtcctgct
    14281 tggcgtagcc gaactcccct gcccaggtgt cctggtcgaa gccctggatc ggcactccgt
    14341 tcgacgcgag catcagtcga acgcgaaacc cctgcacgat ctcgacgtcg gcaagatgcg
    14401 caaggatctc ggtcacggac cacttgcccg gcgccgggcg ttttcccatc tgccgcttgg
    14461 tggcgccttt caccagcgcc gagatcttcc gtggggaaga gacgagaagg tccacgggtt
    14521 tctggtcctc gagataactc aggatcctgc tcacgtactg ctggacggtt tcctgcacgg
    14581 cttcttgacc actccttttg cgggtaaggt agccatgctc gcgatcgatg acaacgggtt
    14641 gctgcccggc gctccggacc catcccgccg cctcagcgct gaggctgcct ccgctgatac
    14701 gcttccgggg gtataccggt gacggacttg aagtctcgga tgaaatgcgc ctggtcgaaa
    14761 tacccgagat ccagcgccag atccgaggag cgcaggtccg gctggcgaag gagccgctcg
    14821 gcggcctcct gaaggcggaa tctcaggatc agctccttcg ggccgatgcc aacctcgcgt
    14881 tgaaatagcc gttgcagacc gcggacatcg aggcccagcg cgcgagctgc gtccgccacg
    14941 gtcctgagcg atcggtccga gatgatccgc tccgcggcga gcgtggcttc cgtcggtccg
    15001 gccgaaggag gccgatcgcg gctgccccat ctctccagca gtgaccgcag gaatcgatcg
    15061 gtgattccgg cccgagcctc ctcgcccggc gcgccctgcg tctccgacgc ccatcggccc
    15121 agtgctttgg gaaaatccag gatcccgttg gtcgggtcct gagccatcac ctgcattcca
    15181 gcgctccgag cgtactgtcg cgtttctaca atccgcggca tcgaacatac tgtaccttgc
    15241 tagccgcggg aaacaccggg cgcgtgcccg tgggtacccg taggaggagg tacgctatgg
    15301 ccgctgagac gttctcgctg agcgtggatg aggtgatcga cgcgcgtccg gctcaggtct
    15361 ggaaggtcct cacggacccc gtgcacgtgg gagagctctt ctggggaagc accgtggaga
    15421 gtgacttcac tcccggccat cctatcgtct ggaagggggt ctggcagggg aagcccttcg
    15481 aggatcgggg ctccatccgg agggccgagg agaacgtgct gctgcaggtc actcactggt
    15541 caccctcctc ggggcccgag accgaggaca accataccct gctcacctgg cgaatccgcc
    15601 cggacggcgg gaaggttcgc gtgacgctgg accacgagaa tatccggacc agggagacgc
    15661 gggaccattc cgagggaatg tggaagcaac tcctcggccg aatgaaggag atgctcgaga
    15721 agccgccgga ctgacttgtc ccggcggggc tggccggata ccgtggaccc atgagcgccc
    15781 ctcccaccgg atcccagccc atcggcccct atcctcagaa ccctcactac ttctcctaca
    15841 agggaagtcc ggtccttctc atcacgagtg atcagcacta cggcgcggtc gtcaacgcgg
    15901 acttcgacta tcgggcgttc ctggatgcca tcgcgtcgta tggcatgaac ttcacacgca
    15961 tctacggcgg ggcgtacatc gagcgggacg gcga
//
LOCUS       DASRZS010000025        11990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4246218, whole genome shotgun sequence.
ACCESSION   DASRZS010000025 DASRZS010000000
VERSION     DASRZS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11990
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4246218"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VFH83_00665"
     CDS             <1..1180
                     /locus_tag="VFH83_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HET6484897.1"
                     /translation="DSGLLTGMLNLIGDAGADYQVVSFDMGKVRTDPSHIELEVRCSD
                     EAQLASLTGLLVDQGCYEKSVGEALLKPAPRDSCVPDDFYATTNRRTQVFLRSAWRDV
                     KDMRMDGVIVARGDDLVCRLIRDVRAGEPIVCTNRSVRIFPLFGEAAAETAEEFGFMA
                     SAVSSERSVDVAIAKVAEELRRQRDSGGRVIVVGGPVVIHTGGGAALASLIEGGYVAG
                     LLAGNALAVHDVESALFGTSLGIALSTGRSVHEGHRNHIRAINTVFAHGSIAAAVRAG
                     AIACGIFHECVKADVPFVLAGSLRDDGPLPETEMNMLVAQRRYSEMLKGATMVIMLST
                     MLHSVATGNMIPAWVKTICVDINPAVVTKLSDRGSGQTIGIVTDVGLFLRSLADKLQR
                     "
     gene            1183..2742
                     /locus_tag="VFH83_00670"
     CDS             1183..2742
                     /locus_tag="VFH83_00670"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET6484898.1"
                     /translation="MSTEAPAPKRELHPLEVKVLLRYPQGQEVSLARLVSDLGYNLGQ
                     ANQALSWLLTKGMVAEKSRTPRVFYEIAELGREALTKGTYEQRIVELLAASGPLTLPQ
                     IAAKLEIDPKEVGSTFGGLSKDKVVAMDEWKRATLAPGGQEAASARLSALRGLLERAQ
                     SAALAEESLSDSEKELVSSQAKKRGSSASLFRRAEREEVAFTVTAAGAEARSRLQSDG
                     ATGEEVGNLTPEMLRSGAWKTARFRRYNIDVPPKRVDVGRKNPYCSFLESLKDKLASL
                     GFEEFDGPLVETEFWNGDALFMPQFHSARDIHDVYYLKCPTHAREIEQPWLDQVAATH
                     ENGWTTGSRGWSYSFDRQFTRRLILRSQGTVLSAKTLPHAKVPGKYFGVVRCFRYDQV
                     DATHLADFYQTEGIVLGEEVNLRTLLGLLRMFAVEVAQAKEVRYVPGFFPFTEPSVEV
                     HIRHPTLGWFELGGSGIFRPEVTEPLGIKVPVLAWGLGIDRMALTSMGLHHIKELFTP
                     SIEEVRSRRMS"
     gene            2746..4446
                     /gene="pheT"
                     /locus_tag="VFH83_00675"
     CDS             2746..4446
                     /gene="pheT"
                     /locus_tag="VFH83_00675"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET6484899.1"
                     /translation="MPKIDVNENLFFQTLGRRFEKVELAELLVRAKAELDDWDTKAGI
                     LRVELNDTNRPDLWSTLGLARQLAIFQTGKIPSYPFFSRAGKLQDTADRRVIVDRGLM
                     EIRPYIVSFVAEGREVTDALLREIIQSQEKLCTIYGRKRKSIAMGVSRADLIQWPVRY
                     SAADPDATRFVPLDFSRPLSMREILTQHPKGKEYGPIVASFERFPLLSDNRGEVLTFP
                     PVINSALIGGVKVGDTRLFIDLTGPDLELLLTAGAITSCDFADMGFTIRPVAVEYPWD
                     TSLGRRVVTPYYFQSDVHVSVAEASRRLGEEFQPEEAAGLIRRMGSDVRVQGDLLTVS
                     PPVYRNDFLHAVDAIEEIMIGRGMESFEPVMPRDFTVGRVSAAELYSRRVRETMVGLG
                     YQEMIYGYLGAKRDFVERMGRDIADLVEIANPMSESADVVRNSILPNLLSSESVSAHA
                     SFPHRIFEVGKVTVQDERENSGTRTYNGLGLLVADREAGFNDVDAHVLALFYYLGKEP
                     RLQPLEDPRFIPGRAAQILLGERRVGILGEIHPQCLENWGIQIPCAAAELDLDLIRGE
                     "
     gene            4464..4952
                     /locus_tag="VFH83_00680"
     CDS             4464..4952
                     /locus_tag="VFH83_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HET6484900.1"
                     /translation="MEAKLDVVPIEIPEGANVIVGQSHFIKTAEDLAEVMATHASAAT
                     FGLAFNEASGPCLIRTEGNDDALVASAVRSARAIGAGHVFVVVMKNVFPISVLGAVKS
                     CQEVCRVFCATANPLQVLVAQTDQGRGVVGVVDGASPKGVETSADRQDRRGMLRKFGY
                     KF"
     gene            4975..6975
                     /locus_tag="VFH83_00685"
     CDS             4975..6975
                     /locus_tag="VFH83_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6484901.1"
                     /translation="MEHLSWGIIGAGGIARQFARGVEHSRTGKLTAIASRTKEKADRF
                     ASEHPLERRHTSYEALLADRAVQAVYIALPHPFHAEWAVKAAEAGKHILCEKPVGLNH
                     AEAMAMVQAARENDVFLMEAFMYRCHPQTARLVELIRSGTIGEVRHIQTSFGFRADFN
                     PEGRLFKNALGGGGVLDVGCYPASMARLIAGAAIGKDFADPIEVKAVGTFQETTRVDE
                     HAVCVARFPGNILATLSTATRVNLDNTVRVQGTTGSILVESPWIPSREGGASRILVWH
                     DGQQTPEEIPIQTSEWLYGIEADTVAANIERRQAPSPAMGWADTLGNMRTLDLWRESF
                     GFQYESEKLDAAWPTVTHRPLAVRKPNPMRYGKIPGVEKPVSRLIMGVDNQRTIAHAS
                     VMFDAFFERGGTAFDSAYVYGGGMCEQVLGRWVRNRGIRDKVVIVDKGAHTPFCTPKD
                     LSRQLLESLDRLGSDHMDLYLMHRDNLEVPVGEFVDVLDEHRSAGRIRAFGGSNWSIQ
                     RLEEANAYARRKGVTGFAALSNNLSLARMVDPVWADCIHVSDKASREWLKRTQTVELS
                     WSSQARGFFTDRASPGDRSDPDLARCWYSDDNFRRKARARDLAYRRMVPEIVIALAYV
                     LCQPFPTFALIGPRSIAEMVQSMAALDVELSAEELEGLWGQD"
     gene            7030..8262
                     /locus_tag="VFH83_00690"
     CDS             7030..8262
                     /locus_tag="VFH83_00690"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6484902.1"
                     /translation="MRKGPLPILFVFVVVDLLGFSLILPLLPYYAKAFSASPLIIGLL
                     GTSNALAQVLAAPLIGRFSDRFGRRPLLLLGTFAGFACFLMLGLARSLTMIFASRIIN
                     GLLGGNQALAQAYITDVTDEKDRARNLGVLGAAFGVGFIIGPALGGFLSSFGNSVPAF
                     AAAGLSLVNFLWIAAALPESLTAERRRAAAGGRQPPITARAMVAAMRRPRVGPLLTTI
                     FVYSLAFGVFTATFALYALRQLGLSAQFTGYILAYVGVILVLVQALAVGPLTARFSEA
                     RIIVMALPIVGFTLLAWGFVPNVPLLLVVFAPMSAAAGILGTVINSALTKSVSRDEIG
                     GTLGISTSLQSLTQVISPVLGGFLLGQLGAWSLGLLGALMMAGLTWFAFKQVLPSERA
                     AGSSGWAAARTEPTRTPE"
     gene            complement(8177..9568)
                     /locus_tag="VFH83_00695"
     CDS             complement(8177..9568)
                     /locus_tag="VFH83_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET6484903.1"
                     /translation="MSKESYRWWTFAVVSMALFMGMLDNLVVTTALPTIQRALGASVA
                     NLEWIVNAYTLGFAVLMIPAAALGERFGRRRVLLTGVALFTGGSALAALASGAGTLIL
                     ARAVQGIGSAMIAPLTLTILVSVFPAERRAAAIGLWSGVSGLGLAAGPLVGGAIVNGL
                     SWNAVFWLNVPIGALLLVLGRLRIEETKTGGRIIDPLSVALVTAGLFSVVFGLIRGNA
                     LGWGSPLIVASLVAGAALLVVFALRQRTSRSPMLDLNLFASRSFSAANAVGFLSSFAM
                     FGSIFFITLFVQGVWGWKPLAAGLGTMPWTGTIMLAAPVAGALAGRLGARRVVVVGMA
                     AQAAALFWIGTAATAGSSYASLLPAFILGGLGMGLSFAPLSATAMAGLPAARSGEASG
                     AYNTLRELGGVFGVSVLGAVFAGVVHAPSQFVDGFHLTLKVAAVILAFGSALSLLLPS
                     RLSPQLAPTEGLA"
     gene            complement(9687..10286)
                     /locus_tag="VFH83_00700"
     CDS             complement(9687..10286)
                     /locus_tag="VFH83_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6484904.1"
                     /translation="MAGGKAAAVDRAARAAAKTRKKPGKPGTRSNAEERREEILDAAI
                     QEFARYGLYGTSVDAIAARVDISQPYIFRLFGTKKDLFLASALRVCERIQEAFHKAVS
                     GQPERPLVAMGHAYVPLLSSRAELMVLLHAFAASEDPEVRKVVGARYQKLWEFVATAS
                     GATREEVRRFFADGMGMTVGAALGLQQLFDFHDAGEPCD"
     gene            10590..11486
                     /locus_tag="VFH83_00705"
     CDS             10590..11486
                     /locus_tag="VFH83_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6484905.1"
                     /translation="MTRRGLLLFASMCLIWGIPYFLIRVAVGQISPVMLVFARTAIGA
                     IILMPVVIATGGLRGLGRKWWAILAFAVAEVGGPWYCLSSAEQHLSSSLAAILISCVP
                     IVGMGVAPLFGNRDRIGPLGIGGLALGLVGVAAIVGLDLQASDPTSLVEMALVVVGYA
                     LGPAIIGRYLTGVPSVTVMGTSLAACAVVYAPLAATQWPSALPSADVLVSVGVLGIVC
                     TAAAFLLFYALIKEIGPVRSTVITYINPAVAAVVGVAVLRETFTVGMGAGFVLVLGGS
                     ILATRRRAQRSKVLRTRRDESM"
     gene            complement(11441..>11990)
                     /locus_tag="VFH83_00710"
     CDS             complement(11441..>11990)
                     /locus_tag="VFH83_00710"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HET6484906.1"
                     /translation="GYRLATAGSPSATVADAAPNAASVAPPLAGPRGLGGWLVLVALG
                     LFLRPVLRLYVLQQDWASFTVGRWFDLTNPSGALYNPLWAPYQFYLIISSILVSAASV
                     LAIVLFFQRRRRFPALFIGLLVLDALTVIATVVLTRALSTQTEQSVGTDVPRAVTQAA
                     VGCALWIPYMLSSRRVRNTFDR"
BASE COUNT         1992 a         4035 c         4012 g         1951 t
ORIGIN      
        1 cgattccggc ctcctcaccg ggatgctgaa cctgatcggc gacgcgggag cggactacca
       61 ggtggtctcc ttcgacatgg gaaaggtgcg caccgacccg tcccacatcg agctcgaggt
      121 gcgatgcagc gacgaggcgc agctcgcctc cctgacgggc ctcttggtgg accagggctg
      181 ctacgagaag tccgtgggag aggcgctcct gaaaccggct ccgcgcgatt cctgcgtgcc
      241 ggatgatttc tacgccacca cgaaccggag gacgcaggtg ttccttcgca gcgcgtggcg
      301 agacgtcaag gacatgcgga tggacggcgt catcgtggcg cggggagacg acctggtctg
      361 ccggctgatc cgggacgtcc gcgcggggga gcccattgtc tgcaccaacc ggtccgttcg
      421 gatcttcccc ctcttcggtg aggcagccgc ggagaccgcc gaggagttcg gcttcatggc
      481 cagcgcggtc tcctcggagc ggagcgtgga cgtggccatc gcaaaggtgg ccgaagagct
      541 tcgacgtcaa cgcgactccg gcggccgcgt gatcgtggtg ggcggcccgg tggtcatcca
      601 caccggcggt ggggcggcgc tggcctcgct catcgaaggt ggctatgtcg ccggcctgct
      661 ggctggcaac gcgctggccg tgcacgacgt ggagagcgcg ctgttcggca cgtccctggg
      721 catcgccctt tccaccgggc gatcggtcca cgaggggcat cgcaaccaca tccgggccat
      781 caacacggtg ttcgcccacg ggtcgatcgc ggccgcggtg cgcgccggcg caatcgcctg
      841 cggcatcttc cacgaatgcg tcaaggcgga cgtgcccttc gtgctggccg gctcgctgcg
      901 ggacgacggt ccgctgccgg aaaccgagat gaacatgctg gtggcgcagc ggcggtactc
      961 cgagatgctc aagggcgcca ccatggtgat catgctgtcc accatgctgc actccgttgc
     1021 cacggggaac atgatccccg cctgggtgaa gacgatctgc gtggacatca atcccgccgt
     1081 cgtcaccaaa ctctccgacc ggggttcagg tcagaccatc ggcatcgtca cggatgtcgg
     1141 gctgttcctg aggtccctgg cggacaaact ccagaggtag cgatgtcgac cgaggcccct
     1201 gctcccaaac gggagcttca tcccctggag gtgaaagtcc tcctccgtta cccccaagga
     1261 caggaggtga gcctcgcgcg gctggtctcc gatctgggct acaacctggg ccaggcaaac
     1321 caggcgctga gctggctcct gacaaaggga atggtggcgg agaaatcccg caccccgcgg
     1381 gtgttctacg agatcgccga gctgggacgg gaggccctca ccaagggtac ctacgagcag
     1441 cgcatcgtgg agcttttggc agcgtccggg cccctgacgc tcccgcagat cgcggcaaag
     1501 ctcgagatcg accccaaaga ggtgggctcc acgttcggcg gcctgtccaa agacaaggtc
     1561 gtagccatgg acgagtggaa gcgcgccacg ctggcgcccg gcggccagga ggcggcgagc
     1621 gcgcggcttt cggcgctgcg cgggctcctg gagcgcgccc agagcgccgc gctggccgag
     1681 gaaagcctga gcgattcgga gaaggagctc gtctcctccc aggccaagaa gcgcggctcc
     1741 tccgcgagcc tcttccgccg cgcggagcgg gaggaggtgg ccttcaccgt tacggcggcg
     1801 ggcgcggagg cgcggtcaag gctccagagt gatggggcca ccggagagga ggtcgggaat
     1861 ctcacaccgg agatgcttcg gtccggggcg tggaagaccg cgcgcttccg ccggtacaac
     1921 atcgacgtcc cacccaaacg cgtggacgtg ggccgcaaga acccctattg ctcattcctg
     1981 gagtccctca aggacaagct cgcgtccctg ggcttcgagg agttcgacgg tccgctggtg
     2041 gagaccgagt tctggaacgg ggacgccctc ttcatgcccc agttccattc cgcccgcgac
     2101 atccacgacg tttactacct gaaatgcccc acgcacgccc gggagatcga gcagccgtgg
     2161 ctggaccagg ttgcggccac ccatgagaac ggctggacta ccggcagccg cgggtggagc
     2221 tactcgttcg accgccagtt cacgcggcgg ctgatcctgc ggagccaggg cacggtgctc
     2281 tccgccaaga ccctcccgca cgccaaggtc cccgggaagt acttcggcgt ggtgcgctgc
     2341 ttccgttacg accaggtgga cgccacgcac cttgccgact tctaccagac ggagggcatc
     2401 gtgctggggg aggaggtaaa cctccgcacg ctcctgggcc tgctgcgcat gttcgcggtc
     2461 gaggtggcac aggccaagga ggtgaggtat gtgccgggat tcttcccatt cacggagcct
     2521 tccgtggaag tgcacatccg tcatcccacc ttggggtggt tcgagctggg cggctcgggg
     2581 atcttccggc cggaggtgac cgagccgttg gggatcaagg tcccggtcct ggcctggggg
     2641 ctcggcatcg accgcatggc gctgacctcc atgggcctcc accacatcaa ggagcttttc
     2701 actccctcca tcgaggaagt ccgatccagg aggatgagct agcccatgcc caagatcgat
     2761 gtcaatgaga acctcttctt ccagaccctg gggcgcaggt tcgagaaggt ggagcttgcg
     2821 gagcttctcg tccgggcaaa ggcggagctg gacgattggg acaccaaggc cggcatcctt
     2881 cgcgtcgagc tgaacgacac caaccggccg gacctgtggt ccacgctggg cctggcgcgg
     2941 cagctcgcca tcttccagac cggcaagatc ccctcgtacc cgttcttctc ccgggccggg
     3001 aagctccagg acaccgcgga ccgacgcgtg atcgtggacc ggggcctcat ggagatccgc
     3061 ccctatatcg tgagcttcgt ggcggagggc cgtgaagtca ccgacgcgct ccttcgggag
     3121 atcatccagt cccaggagaa gctctgcacg atctacggac gcaagcgcaa gagcatcgcc
     3181 atgggcgtgt cccgggcgga cctcatccag tggccggtcc gctacagcgc cgcggatccg
     3241 gatgccacga ggttcgttcc cctggatttc agccggcccc tttccatgcg ggagatcctg
     3301 acccagcacc ccaagggcaa agagtacggc cccatcgtgg cctcgttcga gaggttcccg
     3361 ctcctctccg acaaccgggg cgaggtcctc acgttcccgc cggtcatcaa ttcggcgctc
     3421 atcggaggag tcaaggtcgg agacacgcgc ctgttcatcg acctgaccgg gccggacctg
     3481 gagcttctcc tgaccgcggg cgccatcacc tcctgcgatt tcgcggacat gggcttcacc
     3541 atacggccgg tcgccgtgga gtacccctgg gacacctcgc tgggccggcg cgtggtcacc
     3601 ccgtactact tccagtccga tgtccacgtc tccgtagccg aggcgtccag gcggctcggc
     3661 gaggagttcc agcccgaaga ggccgccggc ctcatccgca ggatggggag cgatgtgcgg
     3721 gtccagggtg acctcctgac cgtgtcaccg ccggtgtacc gaaacgactt cctgcacgcc
     3781 gtggacgcca tcgaggagat catgatcggc cgcggcatgg agagcttcga gccggtgatg
     3841 ccgcgggatt tcacggtggg ccgggtttcg gccgcggagc tgtactcccg acgggttcgg
     3901 gagaccatgg tcggtctggg ataccaggag atgatctatg gctacctcgg cgccaaacgg
     3961 gacttcgtgg agcggatggg tcgggacatc gccgacctgg tggagatcgc caatcccatg
     4021 agcgagagcg cggacgtcgt gcgcaactcc atcctgccga acctcctctc ttcggagtcc
     4081 gtctccgcgc acgcctcctt cccccatcgc atcttcgagg tgggaaaggt gaccgtccag
     4141 gacgagcgag agaacagcgg gacgcggacg tacaacggcc tcggcctcct ggtcgcggac
     4201 cgggaggcgg ggttcaacga cgtggacgcc cacgtgctgg cgctcttcta ctatctgggc
     4261 aaggagcccc gcctccaacc cctggaggac ccgcggttca tccccggccg cgcggcgcag
     4321 atcctgctgg gagagcggcg cgtcggcatc ctgggagaga tccatccgca atgcctggag
     4381 aactggggca tccagatccc ctgcgccgcg gcggagctgg acctggacct gatccgaggg
     4441 gagtgacgga ggcggtcatg gcgatggaag caaagctgga cgtggtcccg atcgagatcc
     4501 cggagggcgc caacgtgatc gttggacagt cccatttcat caagaccgcg gaggacctgg
     4561 cagaggtcat ggccacccac gcttcggcgg cgacgttcgg gctggcgttc aacgaagcct
     4621 cggggccatg cctcatccgc acggagggaa atgacgatgc cctggtggcc agcgcggtgc
     4681 gatcggcgcg ggccatcgga gcggggcacg tcttcgtggt cgtcatgaag aatgtctttc
     4741 cgatctccgt cctgggcgcc gtcaagtcct gccaggaggt gtgccgcgtg ttctgcgcca
     4801 cggccaaccc cctgcaggtg ctggtggcgc agacggacca ggggcgcggc gtggtgggcg
     4861 tggtcgatgg agcatctccg aagggcgtgg agaccagcgc ggatcgccag gaccgccggg
     4921 gcatgcttcg gaagttcgga tacaagttct agcacgaacg ttggggaggt ctccatggaa
     4981 catctgagct ggggcatcat cggcgccggc gggatcgcgc gccagttcgc gcgcggcgta
     5041 gagcattcca ggacgggcaa gctgacggct atcgcgagcc gaacgaaaga gaaagccgac
     5101 cggttcgcct ccgagcaccc gctggaacgc cgccatacca gctacgaggc gctgctggcg
     5161 gaccgcgccg tccaggccgt gtacatcgcg ctcccccacc cctttcacgc ggagtgggcc
     5221 gtaaaggccg ccgaggcggg caagcacatc ctgtgcgaga agcccgtcgg tctgaaccac
     5281 gccgaagcca tggccatggt ccaggcggcc cgggagaacg acgtcttcct catggaagcg
     5341 ttcatgtacc gctgccatcc ccagaccgcc cggttggtgg agctgatccg cagcggcacc
     5401 atcggtgagg tccgccacat ccagaccagc ttcggcttcc gcgcggactt caaccccgag
     5461 ggacgactct tcaagaacgc gctgggcggc ggaggcgtcc tggacgtggg gtgctatccg
     5521 gcctccatgg cccggctcat cgccggagcc gcgatcggga aggacttcgc ggatcccatc
     5581 gaggtcaagg ccgtgggtac gttccaggag accacgcgcg tggatgagca cgcggtttgc
     5641 gtagcgcggt tcccaggcaa catcctggcc acattgtcca ccgctacccg cgtgaacctg
     5701 gacaacaccg tgcgggtgca ggggaccacc ggaagcatcc tcgtcgaatc cccgtggatt
     5761 cccagccgtg agggcggcgc ctcccggatc ctggtctggc acgacgggca gcagacgccc
     5821 gaggagatcc ccatccagac ctcggagtgg ttgtacggca tcgaggcgga cacggtggcc
     5881 gccaacatcg agcggcgaca ggcgccgtca ccggccatgg gctgggcgga tactctgggc
     5941 aacatgcgca cgctggatct gtggcgggag tccttcgggt tccagtacga gtccgagaag
     6001 ctcgatgccg catggcccac ggttacccac cggccgctgg cggtgcgcaa acccaacccc
     6061 atgcgctacg gcaagattcc gggcgtggag aagcccgtct cccggctcat catgggcgtc
     6121 gacaaccaga ggactatcgc ccacgcctcc gtgatgttcg atgcgttctt cgagcggggg
     6181 ggcaccgcgt tcgactccgc ctacgtgtac ggcggaggca tgtgcgagca ggtcctgggc
     6241 cgctgggtcc gcaaccgcgg gattcgggac aaggtggtga tcgtggacaa gggcgctcac
     6301 accccgttct gcacccccaa ggacctcagc cgacagctcc tggagagcct ggatcggctc
     6361 ggctcggacc atatggacct ctacctgatg catcgggaca accttgaggt cccggtcggc
     6421 gagttcgtgg acgtgctgga cgagcaccgg tcggcaggac gcatcagggc cttcggcggc
     6481 tccaactgga gcatccagag gctggaggag gctaacgcat acgcccggcg caagggcgtg
     6541 accgggttcg cggcgctcag caacaacctg agccttgcgc ggatggtgga tcccgtctgg
     6601 gcggactgca tccatgtctc ggacaaggcc tcgcgggagt ggctgaagag gacccagacc
     6661 gtggagctct cctggtccag ccaggcgcgc ggctttttca cggaccgggc ctcaccggga
     6721 gatcgatccg acccggacct cgcccgctgc tggtactccg acgacaactt ccggcgcaag
     6781 gcgcgcgccc gtgacctggc gtaccggcgc atggtgcccg agatcgtcat cgcgctggcg
     6841 tatgtcctct gccagccctt ccctaccttc gcgctcatag gccctcggag catcgcggag
     6901 atggtgcagt cgatggcagc tctggacgtg gagctctccg cggaagagct ggaggggctt
     6961 tgggggcagg actgagctag agatcgcggg tcgattgacg cctggccccg ccggtccgta
     7021 agttacgccg tgcgaaaagg cccgctaccg attctcttcg tcttcgtcgt ggtggatctg
     7081 ctgggcttca gcctgatcct tcccctgctt ccgtactatg ccaaggcgtt ctccgcctcc
     7141 cctctcatca tcgggctcct ggggacctcg aacgcgctgg cgcaggtgct ggcggcgccg
     7201 ctgatcgggc ggttctcgga ccgctttggc cgccgtcctc tgctcctcct gggcacgttc
     7261 gccggctttg cctgcttcct gatgctgggg ctggcccggt ccctgaccat gatcttcgcc
     7321 agccggatca tcaacggcct cctcggcggc aaccaggccc tggcccaggc ttacatcacg
     7381 gacgtaaccg acgagaaaga ccgggcgcgc aacctcgggg tgctgggcgc cgctttcggg
     7441 gtgggcttca tcatcggccc ggcgctcggc ggcttcctga gctcgttcgg caactcggtc
     7501 ccggcgttcg cggctgcggg actgtccctc gtgaacttcc tctggatcgc ggccgcactg
     7561 ccggagtccc tgaccgccga acggcggcgc gccgccgcgg gcggccgcca gccgccgatc
     7621 accgcccgtg ccatggtggc ggcgatgcgg cggccgcggg taggcccgct cctgactacg
     7681 atcttcgtct actccctggc cttcggcgtg ttcacggcaa ccttcgccct ttacgcgctg
     7741 cgccagctcg gactgtcggc ccagttcacg ggatacatcc tggcgtacgt gggggtgatc
     7801 ctggtgctgg tccaggccct tgccgtcgga cccctgaccg cccgcttcag cgaggcgcgg
     7861 atcatcgtca tggccctgcc catcgtcggt ttcacgcttc tggcatgggg gttcgtgccg
     7921 aacgtgcctc tcctgctcgt ggtcttcgcc cccatgtcgg cggcggccgg gatcctgggc
     7981 acggtgatca acagcgccct gaccaagtcg gtctcccggg atgagattgg cggcacgctc
     8041 ggcatctcga cctcccttca gagcctgacc caggtgatct cccccgtgct gggcggattc
     8101 ctgctcggcc aactgggggc gtggtccctc ggtctcctgg gcgcgctcat gatggccggg
     8161 ctgacctggt tcgcgttcaa gcaagtcctt ccgtcggagc gagctgcggg ctcaagcggc
     8221 tgggcagcag caaggacaga gccgacccga acgccagaat gacggccgcc accttgaggg
     8281 tcaggtggaa cccgtccacg aactgggacg gcgcgtgcac cacacccgcg aacacggccc
     8341 ccaggaccga caccccgaac acgccgccca gctcccgcag cgtgttgtag gccccggaag
     8401 cctcaccact gcgcgcggcg ggcaggccgg ccatggctgt ggcggagagc ggcgcgaagg
     8461 acagccccat ccccaggccg cccaggatga aggctgggag caaggaggcg tagctgctcc
     8521 ccgcagtagc cgcggttccg atccagaaca aggcggccgc ctgcgccgcc attcctacca
     8581 ccaccacccg acgcgcgccc agccgcccgg ccagcgcgcc cgctaccggc gcggccagca
     8641 tgatcgtacc ggtccacggc atggtcccca gccccgcggc cagcggcttc catccccaga
     8701 ccccctgcac gaacagggtg atgaagaaga tcgagccgaa catggcgaag ctcgagagga
     8761 acccgacggc gttggccgcg ctgaaggatc gcgaagcgaa caggttcaga tcgagcatgg
     8821 gtgaccggct ggtcctctgg cgaagcgcga agacaaccag gagcgccgca cccgcgacga
     8881 gggacgccac gatgagcggg cttccccagc ccagggcatt cccgcggatc aggccgaaga
     8941 ccaccgagaa gagacccgcg gtgacgaggg ccacgctcag gggatcgatg atccggcctc
     9001 cggtcttcgt ctcctcgatg cgcagtcggc cgagcaccag gagcagggct ccgatcggca
     9061 cgttcaacca gaacacggcg ttccaggaga gcccgttgac gatggcgccg cccaccaggg
     9121 ggcctgcggc cagccccagg cccgagacgc cggaccagag gccgatggcc gcggctcggc
     9181 gctccgccgg gaacacgctc accaggatgg tgagggtgag cggggcgatc atcgcgcttc
     9241 cgatcccctg cacggcgcgg gccaggatga gggtgcccgc tccggatgcc agggccgcca
     9301 gcgccgagcc gcccgtgaag agcgccactc cggtaaggag cacgcgccgc cgcccgaacc
     9361 gctcccccag agccgcggcc ggaatcatca ggacggcgaa ccccaacgtg taggcgttca
     9421 cgatccattc gaggttggcc acgctcgccc ctagcgctcg ctggatggtc ggcaacgcgg
     9481 tggttaccac caggttgtcc agcattccca tgaacaacgc catcgaaacg acggcgaacg
     9541 tccaccaccg atatgactct ttggacatat gtccctcctt gcgggccggc ttcgcacggg
     9601 acccggcacc cccccaacag gacccggccc cccgggcgtg gttagtgact agtcactaac
     9661 ttatccaact aaaaaaagac gcgggatcag tcacagggtt ctcccgcatc gtggaagtcg
     9721 aacaactgct gaaggccgag tgcggctccc accgtcatac ccatgccatc agcgaagaac
     9781 ctccgcacct cctctcgcgt ggcaccggat gccgtagcca cgaactccca aagcttctga
     9841 tatcgggcgc cgacgacctt ccggacctcc gggtcctccg aagcggcgaa cgcatgcagg
     9901 agcaccatca gctcggcgcg actggataga agcggaacgt aggcgtgccc catcgcaacc
     9961 agcggtcgct ccggctgccc gctcacggcc ttgtggaatg cctcctgaat cctctcgcag
    10021 acccgaagcg ccgaggcaag aaagagatcc ttcttggttc cgaaaagccg gaagatgtac
    10081 ggctgggaga tgtcgacccg ggccgcgatg gcgtccaccg acgtcccata cagtccgtac
    10141 cgggcaaact cctgaatggc ggcgtccagg atctcctccc gccgctcctc cgcgttggag
    10201 cgtgtcccgg gtttccctgg cttcttccgc gtctttgcgg cggcccgcgc cgcccggtca
    10261 accgccgccg ctttcccacc cgccatgttg ttagtatgca ctcactaact agaaaagtca
    10321 agaggtgttt gcgcgccgcc ggacaccgct cgccaaccac ctgcaaccgg aggaacggta
    10381 tcgttgcgca ccttcccgag cgttgtggtt cggccaacgc atcgccgaaa ccgtacgggc
    10441 cgtcgaaaca catcgcggga acggacgcta aggtaccgct ccctgcggcc cggcggcgta
    10501 taccgagacc tgcgcccggc cgacaccccc ggcccgcaga ggttggccgc cgtgtgttcg
    10561 ttggtgaggt tccgtggtat acccgacgaa tgactcgccg aggcctgctc ctcttcgcgt
    10621 cgatgtgcct gatctgggga atcccctact tcctgatccg ggtcgccgtc ggccagatct
    10681 ctccggtgat gctcgtgttt gcccgcacgg ccataggggc catcatcctg atgcccgtgg
    10741 tgatcgccac gggcgggttg cgcgggttgg ggcgcaagtg gtgggccatc ctggcgttcg
    10801 cggtcgccga agtcggcggt ccctggtact gcctctccag cgccgaacag catctttcca
    10861 gctccctcgc cgccatcctg atctcctgcg tacctatcgt tggcatgggg gtggcaccgc
    10921 tcttcggcaa tcgcgatcgg attggccccc tggggatcgg cggactggcg ctggggttgg
    10981 tgggcgttgc cgccatcgtg ggactggacc tccaggcgtc cgacccgacg tcccttgtgg
    11041 aaatggcgct ggtggtcgtg ggctatgcgc tcggaccggc catcatcggc cgctacctga
    11101 cgggcgtccc gtccgttacc gtgatgggga cgtccctggc ggcctgcgcg gtggtgtacg
    11161 cgccgctggc tgccacccaa tggccgtccg cccttccgag cgcggacgtg ctggtgtccg
    11221 tcggcgtgct ggggatcgtc tgcacggcgg cggcgttcct gctgttctac gcgctgatca
    11281 aggagatcgg cccggtgcgg tccacggtca tcacctacat caacccggcg gtggccgcgg
    11341 tggtcggcgt cgcggtattg cgcgagacgt tcacggtcgg gatgggagcg ggcttcgtcc
    11401 ttgtcctggg cggctcgatc ctggcaaccc gcaggcgcgc tcagcgatcg aaggtgttgc
    11461 gcacccgtcg ggacgagagc atgtagggga tccagagcgc gcagcctacc gccgcctggg
    11521 tcacggctct gggaacgtcc gtccctacgg attgctcggt ctgggtggaa agggcccggg
    11581 tgagcaccac ggtagcgatc acggtgagcg catcgaggac caggagcccg atgaacaacg
    11641 cggggaaccg gcgtcttcgc tggaagaaca ggacgatggc gagcacgctc gcggcagaca
    11701 cgagaatgct gctgatgatc aggtagaact ggtagggggc ccacaacggg ttgtacaggg
    11761 cgcccgaggg gttggtcaga tcgaaccatc ggccgaccgt gaacgaagcc cagtcctgct
    11821 gcaggacgta gagccgaagg accggccgca ggaacagccc cagcgcgacc agcaccagcc
    11881 atccgcccag accgcggggg ccggccagcg gcggggcgac cgacgccgcg tttggcgccg
    11941 cgtcagccac cgtggccgac ggagagcccg cggtggccag acggtatccg
//
LOCUS       DASRZS010000026         3309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_398286, whole genome shotgun sequence.
ACCESSION   DASRZS010000026 DASRZS010000000
VERSION     DASRZS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398286"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1082)
                     /locus_tag="VFH83_00715"
     CDS             complement(57..1082)
                     /locus_tag="VFH83_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484907.1"
                     /translation="MKIEKSVVGRLPMPYAVSSFVVDGAPCVVCATEDHGPVLWIAPP
                     YEQARELVSGPGGCMSLVADPENPPDLFAIMGCFPGYQFREAGIYRLTRSASRSWSAF
                     RVADLPFAHRMELVRQGTHRFLFAANLAQDKTSPQDWERPGSLWVTDPSAGRGTWKLT
                     PVMEGIHRNHGLLRCRLLGRDSLLLSGAEGLFSIDLESDGPWRSRRVLEREVSEIAVF
                     DLDGDGVEELATIEPFHGDRLRVYHRDGRTGSAWRAAAEAELSFGHGMLATTLAGRRC
                     LLVSNRSGSRDLVSFELRGSGELVPTVIDQGCAAANLLALSTPGRDLLFATNQAAGEI
                     ARYVVSD"
     gene            1160..2050
                     /locus_tag="VFH83_00720"
     CDS             1160..2050
                     /locus_tag="VFH83_00720"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET6484908.1"
                     /translation="MQKIGFLGLGIMGRPMAENLARDFEVLGFDPQQSRLIGLQRVKA
                     ASSAADVARQCSVVCLSLPTAEVVREVALGQHGLGNSLGPGSLVIDLSTGIPSISREV
                     AERLARNQIDFVDAPVSGGEAGAIAATLAIMVGGSPPVVERARAYLSRIGGSVIRVGD
                     VGAGCVAKLVNNMIVGAAFTTIAESFALARANGVDARDLYEAVKGGWAGSKVLDVSAK
                     AICERDYTPGGTIDMLEKDLSYARQLAAQSKVPIPMSALAHEIFVAGQAAGSGRRSQP
                     ALYELWRILGEAASDQNSLK"
     gene            complement(2032..3048)
                     /locus_tag="VFH83_00725"
     CDS             complement(2032..3048)
                     /locus_tag="VFH83_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484909.1"
                     /translation="MRRLVVLPILLLLAGAAWSLDLTSYGLRTVSTATEGSRTVTTFQ
                     DSQGRSLVVSTLVDPPKEVTDRIFQLDRIVYGWRNIATSSIRMTVTDALIEATVTPSK
                     VVVKGGDLLPYIPAGLFLTSTDTVAYDFRVTKDNLFLKIRGPYTNEDEMEAKMASAIA
                     DPNGFLLQSDPSYLIGRLEQAEGRIAVLEHAVQMLTQTLDDRMTSVNQAIDQANQASQ
                     AMADKQSALESAFASKSAEVDQKDADLSAAIDQANKDLAAQDQAISAALDIVRSATMT
                     NQNVSWFVKHRLPQDGVAKVVELKKATPGMTRADVAKELKAAGIKMSDQEIRLVLEYY
                     FNEF"
     gene            complement(3057..>3309)
                     /locus_tag="VFH83_00730"
     CDS             complement(3057..>3309)
                     /locus_tag="VFH83_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484910.1"
                     /translation="FYARDIGENGFVLDARDTGHIAVIVSPSFQVSDGDQGLVFRADN
                     EYIGTIRFTVAGASVTARALEVVPDKDIEPFDKFLIKKM"
BASE COUNT          545 a         1090 c         1113 g          561 t
ORIGIN      
        1 cgctcccgtg gcgtcatgag ggccatcctc tccggaaggc ctggcgcggc tgcccttcag
       61 tcggacacta cgtaccgggc gatctccccc gccgcctggt tcgtggcaaa gaggagatcc
      121 cgtcccggag tcgatagcgc gagcagattg gcggcggcgc agccctggtc gatcaccgtc
      181 ggcacgagct ccccggagcc gcggagctcg aacgagacga ggtcacggct gcccgaccgg
      241 ttgctcacca ggagacagcg cctcccggcg agagtcgtcg ccagcatccc gtggccgaag
      301 gagagctccg cctccgcggc ggcccgccag gcgcttccag ttcttccgtc ccgatggtag
      361 acccgaaggc ggtccccatg gaagggctcg atcgtggcga gctcctccac cccgtctccg
      421 tccaggtcga agacggcgat ctcgctgacc tcgcgctcca agaccctgcg ggaccgccac
      481 ggaccatcgg attccaggtc gatggagaag agcccctcgg ctcctgagag gaggagggag
      541 tcgcgtccca gcaaccgaca gcggagcaac ccgtggttgc ggtggatccc ctccatcacc
      601 ggcgtgagct tccaggttcc gcgccccgcg gaggggtccg tcacccagag gctgccgggc
      661 cgttcccagt cctgagggct ggtcttgtcc tgggccagat tcgccgcgaa gaggaaacgg
      721 tgggtaccct ggcgcaccag ctccatgcgg tgcgcgaacg gcaggtccgc cacgcggaac
      781 gcgctccagg aacgcgacgc ggagcgggtg aggcggtaga tcccggcctc ccggaactgg
      841 tagcccggga agcaacccat gatggcaaac aggtccggcg ggttctcggg gtcggccacc
      901 agcgacatgc atccccccgg cccggacacc agctcccgcg cctgctcgta ggggggcgcg
      961 atccacagga ccggcccgtg gtcttcggtt gcgcagacca cgcagggggc gccgtctacg
     1021 acgaacgacg agaccgcgta cggcatgggt agccggccaa ccacggactt ctctatcttc
     1081 acgctaggct cctggtacct ctatagtcgt tacggggcgg agaacggaca agcacggggc
     1141 accggcaggg ggataagcaa tgcagaagat cggctttctg ggtcttggga tcatgggccg
     1201 ccccatggcg gaaaacctgg ccagggactt cgaggtactg gggttcgatc cccagcagtc
     1261 ccgcctcatc gggctgcagc gggtcaaggc ggcctccagc gcggccgacg tggcgcggca
     1321 gtgctccgtg gtgtgtctgt ccctcccgac cgcggaggtg gtccgggagg ttgccctggg
     1381 gcagcacggg cttggaaatt cgctggggcc gggatccctg gtcatcgatc tcagcaccgg
     1441 catcccttcg atctcccggg aggtggccga acgcctcgcc cggaaccaga tcgatttcgt
     1501 ggacgcgccg gtgagcggcg gggaagcggg agccatcgcc gcgactcttg ccatcatggt
     1561 gggcggctcg cccccggtgg tggagcgcgc gcgggcctac ctctcgcgga tcggcggctc
     1621 cgtgatccgc gtcggggacg tgggagcggg gtgcgtggcc aagctggtca acaacatgat
     1681 cgtcggcgca gccttcacga cgatcgcgga aagcttcgcc ctggcgcggg ccaacggcgt
     1741 ggatgcccgg gacctctatg aggcggtcaa agggggatgg gccggctcca aggtcctgga
     1801 tgtcagcgcc aaggccatct gcgagcggga ctacactccc ggagggacca tcgacatgct
     1861 ggagaaggac ctctcctacg cccgacagct cgccgcgcag agcaaagtcc ccatccccat
     1921 gagcgcgctg gcgcacgaga tcttcgtggc cggacaggca gccggcagcg gcaggcgttc
     1981 ccaaccggcg ctctacgagc tgtggaggat cctgggcgag gccgcctcgg atcagaactc
     2041 gttgaagtag tattccaaga ccagccggat ctcctgatcc gacatcttga ttccagccgc
     2101 cttgagctcc ttggcaacgt ccgcccgagt catgccgggc gttgccttct tgagctccac
     2161 caccttggct accccatcct ggggaagccg gtgcttcacg aaccaggaga cattctgatt
     2221 ggtcatggtg gcggaccgaa cgatgtccaa cgcggcgctg atggcctggt cctgcgcggc
     2281 caggtccttg ttggcctggt ctatggcggc cgacaggtcg gcgtccttct ggtccacttc
     2341 ggccgacttg gaggcgaagg ccgactccag cgcgctctgc ttgtccgcca tcgcctggct
     2401 ggcctgattt gcctggtcta tggcctggtt gacggaagtc atgcggtcat ccagcgtctg
     2461 ggtgagcatc tgcacggcat gctccagcac ggcgattcgt ccctccgcct gctccaaccg
     2521 gccgatgagg taggacgggt cgctctgcag gaggaatccg ttggggtccg cgatggcgct
     2581 tgccatcttg gcttccatct catcctcgtt ggtataggga ccgcggatct tcaggaacag
     2641 gttgtccttc gtgacgcgga agtcgtaggc caccgtgtcc gtggaggtca ggaagagtcc
     2701 cgcgggaatg tacgggagca gatcgccccc tttcaccacc accttgctcg gggtgacggt
     2761 cgcctcgatc agggcgtcgg tgacggtcat ccgaatcgag gaggtggcga tgttcctcca
     2821 gccgtagacg atccggtcga gctggaagat ccggtccgtc acttccttgg ggggatccac
     2881 cagcgtgctg accaccaggg acctcccctg ggagtcctgg aacgtggtca cggtgcggct
     2941 gccctccgtg gcggtggaga ccgtcctcag cccgtagctg gtcaggtcca gagaccacgc
     3001 ggcgccggct agaagcagga ggatcgggag aacgacgagc cttctcatat ccactcctac
     3061 atcttcttga tcaggaactt gtcgaagggt tcgatgtcct tgtcgggaac cacctccagg
     3121 gcccgcgccg tcacggaggc gccggccacc gtgaaccgga tcgtcccgat gtactcgttg
     3181 tccgcccgga agaccagtcc ctggtcgccg tcggagacct ggaagctcgg agagacgatg
     3241 acggcgatat gacccgtgtc ccgcgcgtcc agcacaaacc cgttctcccc gatgtcccgg
     3301 gcgtagaac
//
LOCUS       DASRZS010000027         6235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4631839, whole genome shotgun sequence.
ACCESSION   DASRZS010000027 DASRZS010000000
VERSION     DASRZS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4631839"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VFH83_00735"
     CDS             <1..843
                     /locus_tag="VFH83_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6484911.1"
                     /translation="PIAALDELTFQYANGEVWRFDPPTETYGCVSNPLPAATPEELTR
                     LADQEVREAEAFAPTEADFPEHQRAVERFGATRAIPGGGVSIAVPRDDAWLEAIVVSP
                     DVVGRMLDAQVIRAQKCAPVMARLGLPYCFGGGDFAGRSGPFYSPRFFHQYMLPRLSL
                     ISEACHAAGVFHLFASDGDLWPVARDLFGASGVDGYYEVDGNFMPLARLRDAFPRLKL
                     LGGIRSEVLHRGTEDEVRREAKTAVETARQIRGCIVGCSNQIVAPTPERNLWAMIETV
                     DRYR"
     gene            complement(860..1684)
                     /locus_tag="VFH83_00740"
     CDS             complement(860..1684)
                     /locus_tag="VFH83_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012944972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6484912.1"
                     /translation="MKLSFITLGCPTWDLDTICRRGREYGFDGVDFRGYLDQIDITTL
                     PLFTTQAAQTLRRIHDAGLQVSCVSSSIRVCHPEVRAENLEEARRTVATAHALGAAVV
                     RIFGGGDLEKRSRGELARIGCQTIEEILAIDGARDLRWVFETHDLWIKARDCTLLLDS
                     IESPSFGALWDMGHTSRVGGESPQDTYAAIGKRIGYCHVKDAVHEPGHPKAMEDGWHY
                     VLPGTGQLPLAPSIGILKENGYDGWLACEHEKRWHPNLDEPEVLFPAFVRWARPLI"
     gene            complement(1701..2885)
                     /locus_tag="VFH83_00745"
     CDS             complement(1701..2885)
                     /locus_tag="VFH83_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6484913.1"
                     /translation="MTDKVRLAVIGVGTIGTAHVRDIPRTENAVLAAVCDIDAARARS
                     AGEAAGVPFYTDYRQMLEKERIGGVIVATPHYDHTPISIHAMQRGTSVLVEKPEAVHV
                     RDARRMNEAYQQAKARHPGLIYSIMLMTRTQGPYARLKAMIDAGDLGRLIRTTWIVTD
                     WFRTQAYYDNGDWRATWSGEGGGVLLNQCPHNLDVYQWLVGLPARVTGFAAIGKYHDI
                     EVEDEVTGFFEHENGMVGHFVTTTAESPGTNRLEIVGENGKVVWEGGKILLARNETSM
                     LQFLRNSPQPFARVPSHVEEVAFKDTGESGHRLVIQNFAAAIQDPSTPLIAHGTEGIR
                     GLSLGNAIMLSSWQGKTVELPLDEECYERLLKERIAASKRTAKGKAGTAAIVDASGSF
                     QR"
     gene            2973..3911
                     /locus_tag="VFH83_00750"
     CDS             2973..3911
                     /locus_tag="VFH83_00750"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET6484914.1"
                     /translation="MARSRWTVQEFEELFRSVSNWGSFGRHAARGTLNYVTAEKIRAA
                     ASLVRSGRTVSLSLPLNTVAGPDNPRPVLHHMVRSHQVRSELGGPEFAADFIATEFHG
                     DSFTHIDALCHISYQGRLFDGLSVDEVTSQGARSLDIAALAHGVVGRGVLLDIPRLRG
                     VRWLEPGEAVHREELEAAERAQGVRVGEGDVLLFRTGHHRRRLELGPWNNGYDGAGKA
                     GLHVDAIPFLHERKVAVFFPDGDGDTVPSTVEGVAYPIHALQIGAMGMICADSLQFEE
                     LVPVCEEERRWEMMISAAPLRLPGGTGSVFNPIAIL"
     gene            3923..4996
                     /locus_tag="VFH83_00755"
     CDS             3923..4996
                     /locus_tag="VFH83_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HET6484915.1"
                     /translation="MQPMTVDTIETPALLIDLEAMEANLRLMSDYMRGRRVRLRPHFK
                     THKSPAISHLQIAAGAKGVCCAKVSEAEVLLTSGIRDVLIANQVVDPAKTARLAGLAR
                     LGAKVTVCVDHPSNVETLSQAAVRAGATLNVLVEVDVGMNRCGVKTAEEALALARKIA
                     ASGGLHFEGIQAYEGHLAGEPDIEKRRQGVQEMVRKIGRVKEVIESAGLAVPEISGGG
                     TATYNITGDDTLWTEIQAGSYVFMDSSYGDFGLPFRRSLSILTTVIHTHPGRAVTDAG
                     MKVCSTDHGQPVVKGHPGLEVRTNEEHGIVLDEAGELAYGSRVEFIPSHCCTTVNLHD
                     RYYGVRGGNLEVVWPIPGRGKSQ"
     gene            complement(5001..6032)
                     /locus_tag="VFH83_00760"
     CDS             complement(5001..6032)
                     /locus_tag="VFH83_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6484916.1"
                     /translation="MRDLRFAVFGAGFWANFQLGAWTELPGARCVAVYNRTLSRAREL
                     ASRYGVPAAYDDPEALLSREELDFVDVITHPATHPALVKLVAGRRVPVICQKPMAESL
                     QESRDMVRVCREAGTPFYIHENWRWQEGIRRLKAALDSGIVGAPFRARIDHTSDYPVF
                     SKEPGLKDWKRYILADVGVHLLDTARFLFGEARRLYCQTVRVHKDLKGEDVATVMLSM
                     ESGATVTVYMGFPEHHVERDVFPETRVFVEAERGSIELATDYWLRVTTADGTHVTRKP
                     PKSYPWGEPDYVPFCSAIVPCNANLLAALRGEGTAESTGDDYLKTMILTEAAYESAET
                     GKAIEIPSA"
     gene            complement(6029..>6235)
                     /locus_tag="VFH83_00765"
     CDS             complement(6029..>6235)
                     /locus_tag="VFH83_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484917.1"
                     /translation="AGHGTVSRFDPKRFRAARRWARRTEQAVRALCPSGSLEPDYYHW
                     GTGGPRSSYRREPSLEHSIRRGAQ"
BASE COUNT          973 a         2094 c         2133 g         1035 t
ORIGIN      
        1 ccgatcgcgg cgctggacga gctcacgttc cagtacgcca acggagaggt ctggcgattc
       61 gaccccccca cggagacgta cggctgtgtc tccaatccgc tgcctgcggc aaccccggaa
      121 gagctgacgc gcctggcgga ccaggaggtc cgtgaggcgg aggctttcgc gccgaccgag
      181 gcggactttc cggagcacca gcgcgccgtg gagcgcttcg gcgccacgcg ggccatcccc
      241 ggcggcgggg tctccattgc cgtcccgcgg gacgacgcct ggctggaggc catcgtggtc
      301 tccccggacg tcgtgggccg gatgctggac gcgcaggtca tccgcgccca gaagtgcgcc
      361 cccgtcatgg cgaggcttgg gctcccgtac tgttttggcg gcggtgactt tgcgggacgt
      421 tccgggccct tctattctcc ccgattcttc caccagtaca tgctgccgcg gctctccctc
      481 atctccgagg catgccacgc ggcgggcgtc tttcacctct ttgcgagtga cggggacctg
      541 tggccggtcg ccagggatct gttcggcgcc tcgggcgtgg acgggtacta cgaggtggat
      601 ggcaacttca tgcccctggc ccggctgcgc gatgccttcc cgcgcctcaa gctcctgggc
      661 ggcatccgaa gcgaggtgct gcaccgcggg accgaggacg aggtgcggcg ggaggcgaag
      721 accgcggtgg agaccgcccg gcagatccgc ggatgcatcg tcggctgcag caaccagatc
      781 gtggctccga ctccggagcg caacctctgg gcgatgatcg agacggtgga ccgctaccgc
      841 tagtcgaccg ggccccggtt cagatcagag gccgcgccca gcgaacaaag gcgggaaaca
      901 ggacttccgg ctcgtccagg ttggggtgcc atcgcttctc atgctcgcag gcaagccagc
      961 cgtcgtagcc gttttccttg aggatgccga tggacggggc cagcgggagc tgcccggtgc
     1021 ccggaaggac gtagtgccag ccgtcctcca tggccttggg gtgcccgggc tcgtggaccg
     1081 cgtccttgac gtggcagtaa ccgatccgct tgccgatggc cgcgtaggta tcctggggtg
     1141 actcaccgcc cactcgggag gtgtgcccca tgtcccacag cgcgccgaag ctggggctct
     1201 cgatgctgtc caggagcagc gtgcagtccc gcgccttgat ccacaggtca tgcgtctcga
     1261 acacccaccg caggtccctg gctccatcga tcgccaggat ctcctcgatg gtctggcagc
     1321 cgatccgcgc cagctccccg cgggagcgct tctccaggtc ccctccgccg aagatccgca
     1381 ccacggcggc gcccaacgcg tgcgcggtgg ccacggtccg tcgggcctcc tccaggttct
     1441 ccgcgcggac ctccgggtgg cacacccgga tgctcgagct cacgcaggac acctggagac
     1501 cggcgtcgtg gatccgccga agggtctgcg cggcctgggt ggtgaacagc ggcagggtgg
     1561 tgatgtcgat ctgatcgagg tagccgcgaa agtccacccc gtcgaacccg tactcccggc
     1621 cgcgacggca gatggtgtcc aggtcccagg tggggcagcc cagggtgatg aagctcagct
     1681 tcatggctcc ctcctccggc ctagcgctgg aacgaaccgg aggcgtcgac gatcgcggcg
     1741 gtgcccgcct tgcccttggc ggtccgcttc gaggcagcga tccgttcctt gagcagccgc
     1801 tcgtagcatt cctcgtccag cgggagctcg acggtcttgc cctgccagga ggagagcatg
     1861 atggcgtttc ccagggacag cccccggatg ccttcggtgc cgtgagcgat cagcggggtg
     1921 gacgggtcct gaatggccgc ggcgaagttc tggatcacca gccggtgacc ggactctccg
     1981 gtgtctttga aggcgacctc ttcgacgtgg gacggcaccc gggcgaacgg ctgagggctg
     2041 ttccgaagga actggagcat cgaggtctcg ttgcgggcga ggaggatctt tccgccctcc
     2101 cacaccacct ttccgttctc cccgacgatc tccagacggt tggtccccgg ggattccgcg
     2161 gtggtggtga cgaagtggcc caccatcccg ttctcgtgct cgaagaagcc agtgacctcg
     2221 tcctccacct cgatgtcgtg atacttgccg attgcggcaa agccggtgac cctggccggc
     2281 agccccacca gccactgata gacgtcgagg ttgtgcgggc actggttgag gagcacgccg
     2341 ccgccctcgc cactccaggt tgccctccag tcgccgttgt catagtaggc ctgcgttcgg
     2401 aaccagtccg tgacgatcca ggtggtccgg atgagacggc ccaggtctcc cgcgtcgatc
     2461 atcgccttga gccgcgcgta agggccctgg gtacgggtca tgagcatgat ggagtagatc
     2521 agtccggggt gtcgggcttt ggcctgctgg tacgcctcgt tcatcctgcg ggcgtcccgg
     2581 acgtgcaccg cctccggctt ctcgaccagg acgctcgtcc cccgctgcat cgcgtggatg
     2641 ctgatggggg tgtggtcgta gtgaggcgtc gccacgatga ctccgccgat acgctccttc
     2701 tccagcatct ggcggtagtc cgtgtagaag ggaacgccgg ccgcctctcc ggcggagcgc
     2761 gcgcgggcgg cgtcgatgtc acagaccgcc gccaggacgg cgttctccgt ccgggggatg
     2821 tcgcgcacat gggcggtgcc gatcgtaccg accccgatca ctgcgagcct gaccttgtcc
     2881 gtcatgggtg ggagcctccg ggggtagtat agccgcgccc gtcgaaatgg gaagctcttg
     2941 acccacgggc agcccggagg gaggatggcg ccgtggcccg ttcgcgatgg accgtccagg
     3001 agttcgagga gctgtttcga agcgtgtcca actggggcag cttcggacgg cacgcggcgc
     3061 ggggaacgct caactacgtc accgcggaga agatccgggc cgccgcatcg ctcgtgcggt
     3121 ccgggcgcac cgtgtccctc tccctcccgc tgaacaccgt tgccggtccg gacaatcccc
     3181 ggccggtcct tcaccacatg gtccggtccc accaggtccg ctcggagctg gggggacccg
     3241 agttcgcggc ggacttcatc gccacggagt tccacggcga ctccttcacg cacatcgatg
     3301 cgctgtgcca catctcgtac cagggccggc tgttcgacgg tctgtccgtg gacgaggtga
     3361 ccagccaggg agcgcgcagc ctggatatcg cggccctggc tcacggcgtg gtgggccgcg
     3421 gggtccttct ggacatccct cggctgcgcg gcgtccgttg gctggagcca ggggaggccg
     3481 tgcaccgcga ggagctggag gccgcggagc gcgcgcaggg cgtgcgggta ggggaggggg
     3541 acgtgctgct cttccgcacg ggtcaccacc ggcgccgcct ggagctggga ccgtggaaca
     3601 acggctacga cggggcgggg aaggcgggtc tccacgtgga tgccatcccc ttcctgcacg
     3661 agcggaaggt ggcggtcttc ttcccggatg gggacggaga caccgtgccc agcaccgtgg
     3721 aaggggttgc ctatcccatc cacgcgctgc agatcggggc catgggcatg atctgcgcgg
     3781 acagcctcca gttcgaggag ctcgtgccgg tctgtgagga agagaggcgc tgggagatga
     3841 tgatctccgc ggcgccgctt cgcctgccgg gcgggaccgg ctccgtattc aatcccatcg
     3901 ccatcctgta agagaggtag ccatgcaacc catgaccgta gacaccatcg agactccggc
     3961 cctcctcatc gacctggagg cgatggaggc gaatctccga ctgatgtcgg actacatgcg
     4021 gggaaggcgc gtgcggctgc ggccccactt caagacccac aagagccccg ccatctccca
     4081 cctgcagatc gccgcgggcg ccaagggcgt gtgctgcgcc aaggtttcgg aggcggaggt
     4141 gctcctgacc tcgggcattc gggacgtgct catcgccaac caggtcgtcg atccggccaa
     4201 gaccgcccgc ctggcggggc tggcccgact gggcgcgaag gtcaccgtct gcgtagatca
     4261 cccgtcgaac gtcgagacgc tgtcccaggc cgcggtccgc gcgggcgcca ccctgaacgt
     4321 gctggtcgag gtggatgtag ggatgaaccg ctgcggagtc aagaccgcgg aagaggccct
     4381 ggccctggcc cgcaagatcg ccgcctccgg cggcctccac ttcgagggga tccaggccta
     4441 cgagggccac ctggccggtg agccggacat cgagaagcgc cgccagggcg tccaggagat
     4501 ggtgcggaag atcggcaggg tcaaggaggt catcgagtcg gcgggtctcg cggtccccga
     4561 gatcagtgga ggcgggacgg ccacgtacaa catcacggga gacgacaccc tctggacgga
     4621 gatccaggcc ggctcctacg tgttcatgga ctcttcctat ggggatttcg gcctgccctt
     4681 ccgaaggtcc ctcagcatcc tgacgaccgt catccacacc catcccggcc gcgccgtcac
     4741 ggacgccggc atgaaggtct gttccacgga ccatgggcag cccgtcgtga aggggcaccc
     4801 cggcctggag gtgcggacca acgaagagca cgggatcgtg ctggatgagg caggggagct
     4861 ggcctacggg tcccgggtgg agttcatccc gagccactgc tgcacgaccg tgaacctcca
     4921 cgaccgctac tacggcgttc ggggcggaaa cctcgaggtg gtctggccca tcccgggccg
     4981 cggaaagagc caatagccga tcaggcggac gggatctcga tggccttgcc cgtctccgcg
     5041 gactcatagg ccgcctcggt aaggatcatc gtcttcagat agtcatcccc cgtggattcc
     5101 gccgttccct ctccccgcag cgccgcgagg aggttcgcgt tgcagggcac gatggcggag
     5161 cagaacggga cgtaatccgg ctcaccccag gggtagctct tgggcggctt ccgggtgacg
     5221 tgcgttccat cggccgtggt cacccgcagc cagtagtccg tcgcaagctc gatggagcct
     5281 ctctcggcct cgacgaagac ccgggtctca gggaaaacgt cccgctcaac gtgatgctcc
     5341 gggaagccca tgtacacggt cacggtggct cccgattcca tcgagagcat cacggtagcc
     5401 acgtcctcgc ctttcaggtc cttgtggact ctgacggtct ggcagtacag cctccgcgcc
     5461 tccccaaaga ggaaacgtgc cgtatccagg aggtgaacgc ccacgtccgc caggatgtag
     5521 cgcttccagt ccttcaaccc cggctccttg ctgaacacgg gatagtccga cgtatggtcg
     5581 atacgggcgc ggaacggcgc gccgacgatc cccgagtcga gcgccgcctt cagcctccgg
     5641 atcccctcct gccaccgcca gttctcgtgg atgtagaacg gtgtgccggc ctcacggcag
     5701 acgcggacca tgtcccgcga ctcttggagc gactcggcca tgggcttctg gcagatcacg
     5761 gggacgcggc ggcccgccac gagcttgacc agggccgggt gggtcgccgg gtgggtaatg
     5821 acgtccacga agtccaactc ctcgcgggag agcagggcct cggggtcgtc gtaggcggcg
     5881 ggcactccgt agcgcgaagc cagctcccgc gctcgggaca gcgttcggtt gtacaccgcc
     5941 acgcatcgcg cgccgggaag ctccgtccac gcgccgagct ggaaattggc ccagaacccc
     6001 gcgccgaata ccgcgaatct caggtctctc attgcgcccc ccttcgaatg gagtgctcca
     6061 ggctgggctc ccgacggtac gatgagcgcg ggccgccggt gccccagtgg tagtagtcgg
     6121 gctcaagcga tccagacggg cagagcgcgc ggaccgcctg ctccgttcgt cgggcccagc
     6181 gccgcgcggc acggaagcgc ttcggatcga accgcgaaac ggtgccgtgt ccggc
//
LOCUS       DASRZS010000028         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5401095, whole genome shotgun sequence.
ACCESSION   DASRZS010000028 DASRZS010000000
VERSION     DASRZS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5401095"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VFH83_00770"
     CDS             <1..952
                     /locus_tag="VFH83_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6484918.1"
                     /translation="EDLPLTDSWADRVLTNVERVIYGKRPVIERILVALLARGHVLLE
                     DVPGVGKTILARALSTSLGGSFRRIQCTPDLLPVDVLGVSVLDPKTGRFDFREGPIMT
                     NVLLVDEINRATPRTQSALLEAMGEGSISVEGKPLALPRPFILLATENPVEFEGTFPL
                     PEAQKDRFFLSLRMGYPDPQAESQIMTSQDRVGHPVTSLQPVSDPQTLLGLQEEVLKI
                     EVEQPICDLILSVVAGSRADPRLARGASPRASMALYKGSQARALLRGRSHATAEDVLD
                     LAPSVLVKRIAVRPEQLLKGMTEERVISELLERSAEAMTA"
     gene            949..>2079
                     /locus_tag="VFH83_00775"
     CDS             949..>2079
                     /locus_tag="VFH83_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6484919.1"
                     /translation="MRGSRRLVGPVLGGVFLLAVYLFTPIQPIRASALFLLLVTLVSF
                     AASRLLPRAIAVRRFDTDIRIHRGERLTVRLEIRNLWPIPIRSVTITDQHGGVVADAP
                     PVFQVSLGPREKRILSWSADARERGEISLGWIGLSGPGPFGLNRWARTLEVPFRIIVY
                     PAVSPITLEHRRGLPAGNIAILNRLYEDVTRYRSIREYIPGDELRRINWKVSARLGKL
                     HTMEYLPSLYFPVLVLLNLTPADYPLDGRFFLVERAIEVAASLVLAFAGAQQEVGLLS
                     TGGIAAQAGPLSVPIRPGLAHAMTILESLARVQPPSDEIRIESLATAPSQIAARAGLR
                     VVAVTPLLPSERTGELSRIGRRGSGLEIFYILSAAQRLSDSEA"
BASE COUNT          310 a          709 c          685 g          375 t
ORIGIN      
        1 cgaggatctc cctttgaccg attcctgggc ggatcgtgtc ctcaccaacg tggagcgggt
       61 catctacggc aagcgcccgg tcatcgagcg gatccttgtc gcgcttctgg caaggggtca
      121 cgtgcttctg gaggacgtgc cgggcgttgg caagactatc ctggcgcgcg ccctgtcgac
      181 ctcgctggga ggatcctttc gaaggatcca gtgcacgccg gacctgttgc cggtggacgt
      241 cctgggggtc tccgtcctgg accccaagac aggccggttc gatttccgcg aaggccccat
      301 catgacgaac gtgctgctgg tggacgagat caaccgggcc accccgcgca cccagtccgc
      361 gctcctggag gcgatggggg agggaagcat cagcgtggag ggcaaaccgc tcgcgctccc
      421 gcggccgttc atcctcctgg ccacggagaa cccggtggag ttcgaaggga cctttcccct
      481 tcccgaggcg cagaaggacc ggttcttcct gagtctgcgc atggggtatc ccgatccgca
      541 ggcggagtcc cagatcatga cctcacagga tcgggtaggg catcccgtca cttcactcca
      601 gccggtgtct gatccccaga cgctcctcgg cctccaggag gaggtgctca agatcgaggt
      661 ggagcagccc atctgcgatc tcatcctgtc cgtggtcgcg gggagccggg ccgaccccag
      721 gctggcccgc ggcgcctcgc cccgggcctc gatggcgctc tacaaaggaa gccaggcgcg
      781 cgcgctgctc cgaggcaggt cccacgccac ggcggaggac gtcctggacc tcgctccctc
      841 cgtgctcgtg aagaggatcg ccgtacggcc cgagcagctc ctgaagggga tgacggagga
      901 gagagtcatc tccgagctcc ttgagcggtc cgccgaggcc atgacggcat gagaggatcg
      961 cgccgcctcg tcgggcccgt cctgggaggt gtcttcctcc tggcggtcta cctcttcacc
     1021 ccaatccaac ccattcgcgc gtcggccctg ttcctgctcc tggtgacgct cgtctccttc
     1081 gcagcctcga ggcttctccc ccgggccata gcggtccgga ggttcgacac cgatatccgg
     1141 atccatcgag gcgagcgtct caccgtgcgc ctggagatcc gcaacctctg gcccatcccc
     1201 atacgctcgg tgaccatcac cgatcagcat ggcggcgtgg tggcggatgc gccgccggtg
     1261 ttccaggtct ccctggggcc tcgggagaag cgcatcctga gctggagcgc ggacgcgcgc
     1321 gagaggggcg agatctcgct gggctggatc ggcctgtcgg ggccggggcc gttcggtctc
     1381 aaccgctggg ccaggaccct cgaggtaccg tttcgcatca tcgtctatcc cgcggtatct
     1441 cccataacgc tggagcaccg gcgcggactc ccggcaggca acatcgccat cctgaaccgg
     1501 ctctatgagg atgtgacccg ctaccgctcg atccgggagt acatcccggg agacgagctt
     1561 cgtcgcatca actggaaggt gtcggcgcgg ctcggcaagc tgcacaccat ggagtacctg
     1621 ccgtccctgt acttcccggt attggtcctg ctcaacctca cgcccgccga ttatccgttg
     1681 gacgggcgat tcttcctggt ggagcgggcc atcgaggtgg cggcgtccct ggtgctcgcc
     1741 ttcgcggggg cgcagcagga ggtgggcctg ctttccaccg gagggattgc cgctcaggcc
     1801 gggcccctct cggttccgat tcgccccggg ctggctcacg cgatgacgat tctcgagtcc
     1861 ctggcgcgtg tccagccgcc gtcggatgag atcaggatcg agagcctggc aacagcgcct
     1921 tctcagatcg cggcccgagc gggactgcgc gtcgtcgccg tgacgccact cctcccttcc
     1981 gagcggaccg gagagctgtc ccggatcggc cgtagaggct cggggctgga gatcttctac
     2041 atcctgtccg cggcgcagcg gctctcggat tcggaggcc
//
LOCUS       DASRZS010000029         5154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3093432, whole genome shotgun sequence.
ACCESSION   DASRZS010000029 DASRZS010000000
VERSION     DASRZS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3093432"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VFH83_00780"
     CDS             complement(<1..348)
                     /locus_tag="VFH83_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018870120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET6484920.1"
                     /translation="MNNDAATRWTAPAGAVRVQDLFDAMEEGFALHEIILDADGNPVD
                     YRFLTVNHAFERQSGLTASTVIGRTVREVLPGIERIWIERYGRVALTGHAERFEESAA
                     GLGRRLEVSAYCPS"
     gene            complement(414..2609)
                     /locus_tag="VFH83_00785"
     CDS             complement(414..2609)
                     /locus_tag="VFH83_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6484921.1"
                     /translation="MKNMKLAAKIGVGFGLVIIIAMGLGGVAIMNMVGVQGDAQRLNA
                     ETVPQVAVANNVERNSLQTMYNMRGFALSEDRTYWDQAQKYLSDVKKYLADAQTLAAK
                     YPRLIELRKNATEAAARVADYEKLAQQTSDVTDNILESRKAQDAAAPEFMKACLDYET
                     RNIADLQSAIARRASPSVQRNILAEITTINEIIDLGNGLRIANFKAQATRDYSILQAG
                     LDQFNQLEGKVTALRGLSPDKNDLADYDTILKAGADYRAACEAILADGKQITDLNKSR
                     GDAATAVLDAAQKTSESGLSDARSVTVLTAARLYSAVIVLVIGLAVAALIGIAIAVAI
                     TRAITAPLSRGVSFAQRVAGGDFTQQLSIHQKDEVGILADALNGMCVKLRTMVSTVQE
                     NAEQVASSSEQISASSQKLAEGAQSQASTLEETSASVEQLTASVDQVAEHAQSQASAV
                     QQGTASMAQVQRSIEDVSRNLTEIATLARTSVDNAVNGAKAVQEVVSGINRIAESSEK
                     IGGIIGVISDIADQTNLLALNASIEAARAGEHGRGFAVVADEVSKLAERSASSTKEIE
                     NLIRESVRNVSEGVKVAEGSQTAMEQIRDASQKVKEMIGSLSESMSQQVTASQELSKA
                     LDNINDMSLSISAATEEQTTNAKQVSKAVENVNDITQTAASSAEEMSAATEQLATMAQ
                     ELQRLVAQFRIDAGRDAPAALVAAAGGNGSGNGHAGRNGTASRTLPAVG"
     gene            complement(2635..3126)
                     /locus_tag="VFH83_00790"
     CDS             complement(2635..3126)
                     /locus_tag="VFH83_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET6484922.1"
                     /translation="MSITAEMDLTGVRQLISFTVGAEEYGLELRQVKEIIRMGQITRL
                     PRAPECVKGIINLRGQVIPIVDLRQRFGLPRAEDTVMTRVIVVEAGGVPVGLVVDSAS
                     QVVRVPMDQIEPPPPFLGTHAGDFIDGVGKLDDRIIITVDAARILSLDELSEISGSLK
                     AAV"
     gene            complement(3312..4208)
                     /locus_tag="VFH83_00795"
     CDS             complement(3312..4208)
                     /locus_tag="VFH83_00795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6484923.1"
                     /translation="MNFPLTAQMIDKIAFAMEDQKEKFAVDVDSGELVAVSGLPALSE
                     EKHVRLPRWGSAEGFHLMESFVTSLDNPAYREQLSQSLATGKGVFRAFKDALRQNKEI
                     EKLWFTYKERRLRGVIVSWYNANREARGLARLPAEPEETDELLQSDFSFTWEVGERRD
                     SVQQLDRDAFFELFPSETPTVLERRYREKRQDVPPAGDAASPILIAETPSGELAGIVW
                     GVIQGRTVHVIQLAVFPAFRGTGLSEILLRQFLAAMRSRGMERLVAELMGKSLRFSDF
                     FQSMGFLPVAQVMECSLGELSY"
     gene            4336..>5154
                     /locus_tag="VFH83_00800"
     CDS             4336..>5154
                     /locus_tag="VFH83_00800"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET6484924.1"
                     /translation="MSIIEYVEAREILDSRGNPTVEVDVVLEDGSMGRAAVPSGASTG
                     EHEALELRDGDKARFLGKGVLKAVQNVNTIIAPEIVGLDALEQVDVDHTMMELDGTET
                     KSKLGANAILGVSMATARAAADFLGVPLFKYLGAYHASLMPVPMANIINGGAHADSSV
                     DFQEFMIMPVGAPSIREAVRMMAEIFHTLKGVLKAKGYNTGVGDEGGYAPSLKSNDEA
                     VEVILQAIEKAGYKPGDNVMIALDPAASEFYDVEKKRYVFKKSDKRELTSEEMVD"
BASE COUNT          839 a         1672 c         1686 g          957 t
ORIGIN      
        1 ggaggggcag tacgcgctga cttccaggcg ccggcccagc ccggcggcgg actcctcgaa
       61 ccgctcggca tggcccgtga gcgcgaccct gccgtagcgc tcgatccaga ttcgctcgat
      121 tccgggcagg acctcgcgca cggtgcggcc gatgaccgtg gaggccgtca gtcccgactg
      181 gcgctcgaat gcgtggttca ccgtcaggaa tcggtagtcg accgggtttc cgtcagcgtc
      241 gaggatgatc tcgtgaaggg cgaacccttc ctccatcgcg tcgaaaaggt cctggacccg
      301 gaccgctccc gcgggagcgg tccatcgcgt tgccgcgtcg ttgttcatgt tgcttcccac
      361 cggggccgcc cggcgctttg ggagtcggcg ccgcgcggcc gcgggaaaac gccctacccg
      421 accgccggaa gcgtccggga agccgtgccg ttgcggccgg catggccgtt gccactgccg
      481 ttcccgccgg ccgcggccac gagtgccgcc ggcgcatcac ggcccgcgtc gattcggaac
      541 tgcgccacga ggcgctgcag ctcctgggcc atcgtggcaa gctgctccgt ggcggcggac
      601 atctcctccg cggaggaagc ggcggtctgc gtgatgtcgt tcacgttctc cacggccttg
      661 gagacctgct tggcgttcgt ggtctgctct tccgtggcgg cggagatgct gaggctcatg
      721 tcgttgatgt tgtccagggc cttggacagc tcctgggacg cggtcacctg ctgggacatg
      781 gactccgaaa ggctgccgat catctccttc accttctggg aggcgtcccg tatctgctcc
      841 atggccgtct gggatccctc ggcgaccttc acgccttccg acacgttgcg cacgctctcc
      901 cggatcaggt tctcgatctc tttcgtggac gaagcgctgc gctcggcgag cttgctcacc
      961 tcgtcggcca cgaccgcaaa gccgcggcca tgctcgccgg cgcgcgccgc ctctatggaa
     1021 gcgttcagcg ccagcaggtt cgtctggtcc gcgatgtcgg agatgacacc gatgatcccg
     1081 ccgatcttct ccgagctttc ggcaatccgg ttgatcccgg acaccacttc ctggacggcc
     1141 ttcgcgccgt tgacggcgtt gtccacggag gtgcgggcaa gggtggctat ctccgtcagg
     1201 ttgcgggaca cgtcctcgat cgatctctgg acctgtgcca tggaagcggt cccctgctgc
     1261 acggcggatg cctgggactg cgcatgctcg gccacctggt ccacggaagc agtgagctgc
     1321 tccaccgagg cgctggtctc ctccagcgtg gaggcctggc tctgggcgcc ctccgcgagc
     1381 ttctgcgagc tggccgagat ctgctcgctg gaggaggcga cctgttcggc attctcctgg
     1441 accgtggaca ccatggtgcg cagcttcacg cacatgccgt tcagcgcatc cgccaggatt
     1501 cctacctcgt ccttctggtg gatggaaagc tgctgggtga agtcaccgcc ggccacacgc
     1561 tgggcaaagc tcacgccgcg tgacagcggc gcggtgatgg cacgggtgat ggccaccgcg
     1621 atggcgatac cgatcagggc ggcaacggcc aggccgatca ccaggacgat caccgcggag
     1681 tacagccgcg ccgccgtgag aacggtgacg ctgcgcgcgt ccgagaggcc ggactcggag
     1741 gtcttctggg ccgcatccag caccgccgtg gcggcgtccc cccggctctt gttcaggtcc
     1801 gttatctgct tgccgtcggc gaggatggct tcacaggcag cgcggtagtc ggcaccagcc
     1861 ttgaggatcg tgtcgtagtc cgcgaggtca ttcttgtccg gggagagccc tctcagcgcc
     1921 gtgaccttcc cttccagctg gttgaactgg tccaggccgg cctggaggat gctgtagtcc
     1981 cgggtggcct gggccttgaa attggcgatt cgaagaccat tccccaggtc gatgatctcg
     2041 ttgatcgtcg tgatctccgc caggatgttc ctctgcacgg agggagaggc gcgtcgggcg
     2101 atggcgctct gcaggtcagc aatgttgcgg gtttcgtagt ccaggcacgc cttcatgaac
     2161 tcgggggcgg ctgcatcctg cgcctttcgg gactccagga tgttgtcggt gacatcggag
     2221 gtctgctggg cgagcttctc gtaatcggct acgcgcgccg cggcctccgt ggcgttcttt
     2281 cgaagctcga tgagccgggg gtatttcgcc gccagcgtct gggcgtccgc gaggtacttc
     2341 ttcacatcgc tcagatactt ctgtgcctgg tcccagtagg tgcggtcctc ggacagcgcg
     2401 aagcccctca tgttgtacat ggtctgcagc gagttgcgct ccacgttgtt ggctacggcg
     2461 acctggggaa cggtctccgc gttcaggcgc tgggcatccc cctgcacgcc caccatgttc
     2521 atgatggcca cgcccccgag ccccatggcg atgatgatca ccagaccgaa cccgacgccg
     2581 atcttcgcgg cgagcttcat gttcttcatt cccagtctcc ttcgtgtttg ttggtcagac
     2641 ggctgccttc aggctgccgg aaatctcgct cagctcgtcc agggagagga tccgcgcggc
     2701 gtccacggtg atgatgatcc ggtcatcgag cttgccgacg ccgtcaatga agtcaccggc
     2761 gtgcgttccc aggaacggag ggggcggctc tatctggtcc atggggacgc ggacgacctg
     2821 gctggcggaa tccaccacga ggcccaccgg gacgccaccc gcctccacca cgattacgcg
     2881 ggtcatgacc gtgtcctcgg ccctgggcag cccgaagcgc tggcgcaggt ccacgatagg
     2941 aatcacctgt ccgcgcagat tgatgatgcc tttcacgcat tcgggggccc gggggagccg
     3001 ggtgatctgg cccatccgga tgatttcctt gacctggcga agctccaggc cgtactcctc
     3061 cgcgccgacg gtgaagctga tcagttgcct cacccccgtg agatccatct ctgcggtaat
     3121 gctcacttgc cacttcctcc cgggcctgtc tctttcaaga aaagtgccaa cgtggtgaga
     3181 atggcagttt tttcggaatc ccatttattt gtggaagtta cagggccttc ccggagagct
     3241 tggcgcgttg agccgccggt agcatcgttc cacttttgac cacctggaac gacgtcggtg
     3301 gaacggcgga gctagtagga gagctcgccc aggctgcact ccatcacctg ggctacggga
     3361 aggaatccca tggattggaa gaagtcggag aacctcaggg acttgcccat cagctcggca
     3421 acgaggcgct ccatgccgcg gctgcgcatg gccgcgagga actgccgcag caggatctcg
     3481 ctgagcccgg tgccgcggaa cgcggggaag acggcaagct ggatgacgtg gacggtgcgg
     3541 ccctggatca cgccccatac aatcccggca agctcgccgg aaggtgtttc cgcgatcagg
     3601 atcggggagg cggcgtcccc ggcgggcggc acgtcctggc gcttctcccg gtagcgccgc
     3661 tccaggacgg tcggagtctc tgaggggaac agctcgaaaa aagcgtcccg gtcaagctgc
     3721 tggaccgagt ccctcctttc ccccacctcc caggtgaagg agaaatcact ctgcaggagc
     3781 tcgtcagtct cctctggctc cgcgggcagc cgggcgaggc cccgtgcctc ccggttggcg
     3841 ttgtaccagc tcacgatcac gccgcgcagg cgccgttcct tgtaggtgaa ccacagcttc
     3901 tcgatctcct tgttctgccg aagtgcgtcc ttgaaagcgc gaaacacgcc cttccccgtt
     3961 gcgaggctct gggagagctg ctcccggtag gcggggttgt ccagggaggt gacgaagctt
     4021 tccatcaggt ggaagccctc ggcggagccc catcgcggca gccggacgtg cttctcctcg
     4081 gagagcgccg gcaggccgga gacggccacc agctcgccgg agtccacgtc cacggcaaac
     4141 ttctccttct ggtcctccat ggcgaaggcg atcttgtcga tcatctgcgc cgtcagcggg
     4201 aagttcatgg ccccactgta cggcaaagac tccgccgcgc gcaatggcgc cccgcggatt
     4261 gaccaccgtc gcgccccccg gcatactcgg gaaaacgttg cggtcaccac ccatttgcag
     4321 gcaaaggagc tttctatgag catcatcgag tacgtggaag ccagggaaat cctcgactcc
     4381 cggggcaacc ccaccgtgga agtagacgtg gtcctggaag acgggtccat gggccgtgcc
     4441 gctgtcccgt cgggcgcctc cacgggagag cacgaagcgc tggagctgag ggacggggac
     4501 aaggcccggt tcctcggcaa gggcgtgctc aaagcggttc agaacgtgaa caccatcatc
     4561 gccccggaga tcgtggggtt ggacgccctg gagcaggtgg acgtggacca cacgatgatg
     4621 gagctggacg ggaccgaaac gaagtccaag ctcggcgcca acgccatcct gggcgtgtcc
     4681 atggccaccg cgcgcgccgc ggcggatttc ctgggcgtgc cgctgttcaa gtacctcggt
     4741 gcctaccacg cctccctgat gccggtgccc atggccaaca tcatcaacgg cggggcccac
     4801 gcggacagct cggtcgattt ccaggagttc atgatcatgc cggtgggcgc cccctccatc
     4861 cgggaagcgg tgcgcatgat ggcggaaatt ttccacaccc tgaagggcgt tctgaaggcc
     4921 aaaggctaca acacgggcgt aggggacgag ggcggctacg cgccgagcct gaagagcaac
     4981 gacgaggccg tcgaggtgat cctccaggcc atcgagaagg ccggctacaa gcccggtgac
     5041 aacgtgatga tcgcgctgga cccggcggcc tccgagttct atgacgtgga gaagaagcgc
     5101 tacgtgttca agaagtcgga caagcgcgag ctcacgtccg aggaaatggt ggac
//
LOCUS       DASRZS010000030         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5017622, whole genome shotgun sequence.
ACCESSION   DASRZS010000030 DASRZS010000000
VERSION     DASRZS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5017622"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VFH83_00805"
     CDS             <1..641
                     /locus_tag="VFH83_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484925.1"
                     /translation="FTGDRRIGAFFLPVGAGWGGRSVFRELVQHPDGTLGTRWPQEMI
                     PRTGDPVNLGFSALDSVASGDPSRIHLAAAGFTSAFLTGAPRNALIRFRVTAARGTYV
                     FGVTVRAGQDPRKALSLEFEPSRRKVGWRSAADHAWKESEIAALYQVDGLEEPATVEV
                     IIVDDLLDVCINGQRTLINYQVAGDGDGLLFYCHAGEVTFDSITVRPVLDSI"
     gene            706..1986
                     /locus_tag="VFH83_00810"
     CDS             706..1986
                     /locus_tag="VFH83_00810"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HET6484926.1"
                     /translation="MTADWTHLCKKIGVRLAGTENELRAAEYIRDRLRSAGCAGATLE
                     PFPCRSLRESQVQVSVLDGAVWKPVECAAITGTNSTPGSEPVEGEIVWIEMPEQMPQV
                     TTLRGKIAILVGPLPTDLGLHKRLVKAEPLAVIHVDHRLPFSWVKQDGAYPLWVQKYG
                     FPPTVTIPYREAWDWKRRDARRARVLVRQTLAAADSCNVVAELPGTEPQSGALLLCAH
                     HDTQAANEGADDNASGVVAVLELARALARVPHRRTIRFVSFGTEEQLSVGAAAYAKRH
                     ARELDRIALVLNFDSVSSVLGHFVLHRAANPRLSALVESRLLESGLPVVASDEVSPFA
                     DHFIFTAQGVGAFWFMRESFPAGRWQHHSVHDNLANVSASELSRLLRAACELIDTLSE
                     APRALFRYHPSSEVRRRIQTLALELYGVRGRWGT"
BASE COUNT          319 a          678 c          686 g          374 t
ORIGIN      
        1 cgttcacggg agaccgccgc atcggcgcct tctttcttcc ggtgggagcg ggctggggcg
       61 gaaggtcggt atttcgggag ttggtccagc atcccgacgg cactctgggg acccgctggc
      121 ctcaggagat gatcccgcgg accggagatc cggtgaatct tgggttttcc gcactggact
      181 ccgtcgcgtc gggcgatccc tcgcggattc atctagccgc ggcaggtttc acctcggcat
      241 tcctgacggg cgctccccgg aatgcgctga tccgctttcg ggtcaccgcg gcgcggggca
      301 cctacgtgtt cggcgtgacc gttcgggcgg gccaggaccc ccgcaaggct ctgagcctgg
      361 agttcgagcc ttcccgacgc aaggttgggt ggcgctccgc agcggatcac gcgtggaagg
      421 agagcgagat cgccgccctg taccaggtgg acggcctgga ggagcccgcg accgtggagg
      481 tcatcattgt cgatgacctg cttgacgtct gcatcaacgg gcagaggacc ctcatcaatt
      541 accaggttgc tggagacggt gacgggttgc tattctactg ccatgcagga gaggtcacct
      601 tcgactcgat cacggtccgg ccggtcctcg actcgatcta gcgcatccca ccgcggggtc
      661 cgaaagcccc tgccagccga gccctacgcg ccttcccttc ccgagatgac cgcggactgg
      721 acgcatctct gcaagaagat cggcgtccga ctggctggca cggagaacga gcttcgggcc
      781 gccgaataca tccgcgatcg cctgcgcagc gccggctgcg ccggcgccac tctggaaccc
      841 ttcccgtgcc gatcgctgcg ggagtcccag gtccaggtct ccgtcctgga tggcgcggtc
      901 tggaaacccg tggagtgtgc ggcgatcacc gggaccaaca gcacccccgg atccgagccc
      961 gtcgaagggg aaatcgtgtg gatcgagatg cccgagcaga tgccgcaggt gaccacgctg
     1021 cgcggcaaga tcgcgatcct ggtcggtccg ctcccgaccg acctcgggct gcacaagcgg
     1081 ctggtcaagg ccgagccgct tgccgtcatt cacgtcgacc atcggcttcc gttctcctgg
     1141 gtgaagcagg atggcgcgta tcccctctgg gtgcagaagt acgggtttcc ccccacggtc
     1201 accattccgt atcgggaagc ctgggactgg aagcggagag acgccaggcg cgctcgggtc
     1261 ctggtccggc agaccctggc agccgcggac tcctgcaacg tggtggcgga gctgcccggc
     1321 acggagcctc agagcggggc ccttctcctc tgcgctcacc acgataccca ggcagccaac
     1381 gagggggcgg atgacaacgc ctccggcgtg gtagcggtcc tggagcttgc tcgggccctc
     1441 gcgcgcgtgc cccaccggcg gaccatccgg ttcgtgtcgt tcggcaccga ggaacagctt
     1501 tccgtcggcg ccgctgcgta tgccaaacgc cacgcccgag agctggaccg gatcgccctg
     1561 gtcctcaact tcgacagtgt ctcttcggtg ctggggcact tcgtgcttca ccgggctgcg
     1621 aacccccgct tgagcgccct ggtggagtct cggcttctgg agtccgggct tccggttgtg
     1681 gcctccgacg aagtatcccc gttcgctgac catttcatct tcacggctca aggggtcggg
     1741 gcattctggt tcatgcggga gagtttcccg gcaggtcgct ggcagcatca cagcgtgcac
     1801 gacaacctgg cgaacgtgtc agcttcggag ctttcccggc ttctacgggc cgcctgcgag
     1861 ctgatcgaca cgctctcgga ggcgccacgc gcactctttc ggtaccaccc gtcgtccgag
     1921 gtgcgccgca ggatccagac cctggctctg gagctttacg gtgtgcgggg tcgctggggc
     1981 acgtgaggcg atcaggaccc aggtacggaa ctccggctgg attcgggcag gtcggttata
     2041 gaacgaaagg ttaccgg
//
LOCUS       DASRZS010000031         4342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3863496, whole genome shotgun sequence.
ACCESSION   DASRZS010000031 DASRZS010000000
VERSION     DASRZS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3863496"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..648)
                     /locus_tag="VFH83_00815"
     CDS             complement(121..648)
                     /locus_tag="VFH83_00815"
                     /inference="COORDINATES: protein motif:HMM:NF042671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6622 family protein"
                     /protein_id="HET6484927.1"
                     /translation="MRRVVMEALVQIVTHTPVYVFPALAYGVFAGIRGFRPRAVSIPT
                     MPILPAVFLVLAITSLVTAASVLPTAVFAWVVALAAGIALGASLMAARVLQVDRDRSR
                     VTVGGSATALVLFLAIFALKYMNGVVHARNAGLAAAPAFILAVTVLSGVSTGIMVGRV
                     AKLFSVYFRARAAVA"
     gene            complement(798..870)
                     /locus_tag="VFH83_00820"
     tRNA            complement(798..870)
                     /locus_tag="VFH83_00820"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(835..837),aa:Lys,seq:ttt)
     gene            complement(994..2178)
                     /locus_tag="VFH83_00825"
     CDS             complement(994..2178)
                     /locus_tag="VFH83_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484928.1"
                     /translation="MNRPRRVFLAFLLVLGLTAPVFGQTTVVAPVEIPSARASALGGL
                     HAALADDLGSLFSNPAGFALAGPQLSVSEVTLHLTGPVFSIADLVYKGTTGTDFTTLL
                     QTPSVISLLSSLFASGTLNGPLALGYVGGGLGFGIFNASNVTFTTVGTPPTVTASIGE
                     DVIFAGGYAFAIPLPASWKSKLDIGATLKAFARADVTLRESILDVLTLLAAPDVSIIY
                     NQPFELDAGIGVDVGIRYSWNDTISFGVVGRNLYAPALVNSYTTFSAFGQGAAPTMSF
                     GTVPLDLAAGFAFTPNLGALEPYLGGLKVLLDYQDILDFLTHPTTASNPVLHVGLGIE
                     FQLLQILALRAGFNEGYFSAGAGLNLSVFRLNLTMYGSELSLEPGLRPAYNLIVGLEF
                     RY"
     gene            complement(2199..2807)
                     /locus_tag="VFH83_00830"
     CDS             complement(2199..2807)
                     /locus_tag="VFH83_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484929.1"
                     /translation="MNGRGLAVRSALVIVFSLLTLVGCQEIFTYTPLAFLRRDPSTLS
                     PAQKVEAAKEALQSGDPSAMKAAYDAIKSDTSPDATYQAAQLGVELSGVGTLLTQVAT
                     GQLSLSGNAIGDFVAATAGIDTSYLKDAGTRLSTLNTQSYPLTTNDRMMGAVGLVLQA
                     AGPSTNLTSLTASDVAPAVNLLAPISTTDPYANLLSSYLSGL"
     gene            complement(2877..4061)
                     /locus_tag="VFH83_00835"
     CDS             complement(2877..4061)
                     /locus_tag="VFH83_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484930.1"
                     /translation="MSNRARLVALILLTAVGTAYAAQVAEIPFAPITPSIIAQGGTGV
                     ASAQGWDSFFLNPAGFSRSNGSLTIATSGLWAYLRPDQALSMGVKALSTRPSPADYAS
                     FLASQVTGGGFGLGSSVGTGYVGNGLGLGVGLIVDSLAHGPTLLGATGDLTGTIGFIG
                     GMSVPLSLAPGLTVHVGGAVRPMIRIHVLMDNAATLGALGAIASGGDVFAALNSASAL
                     YGVGIGVDVGTILEAGWFTFGIACRDLGGTQFRYSENALASLEQSFSSQGKLPAGTAS
                     LSDSYVIPMDLGVGVTLHPDLGRFSGWIDPSLSLDLDDLTGLISGARTVWTALHVGGQ
                     ARLLSLFFVQAGVNQGYFTAGGGLKIPLLDVSFAVFTQELGYYVQDWPRAGATLDVSI
                     RL"
     gene            complement(4078..>4342)
                     /locus_tag="VFH83_00840"
     CDS             complement(4078..>4342)
                     /locus_tag="VFH83_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484931.1"
                     /translation="SIPPIPGGVDMGDVAQKALTAHVASFVVASVSGLVGSSQAVPQI
                     YDVLAGKPSTIPAGYSVGDPYASPPTWLVNIVHAGLPSELRPF"
BASE COUNT          840 a         1386 c         1475 g          641 t
ORIGIN      
        1 aggcaagtcc gcgactcacc gcggggttgg acactgggtg acccaggaga gacccggcga
       61 gctcaatcgg cacctgctcg atttcctcgc cgggatcggg agcgtgcttc gatagcacgt
      121 tcaggccact gccgcccgcg ccctgaagta gaccgagaag agcttcgcga ccctccccac
      181 catgatgcct gtgctgaccc ccgagagcac cgtcaccgcc aggatgaacg cgggggcagc
      241 ggcgagacct gcgttcctgg catgcaccac accgttcatg tacttcaagg cgaagatggc
      301 caggaagaga acgagcgcgg tcgcgctccc gccgacggtc acgcggctgc gatcacgatc
      361 gacctggagc acacgcgccg ccattagcga tgcgcccagg gcgatgcccg ccgcgagcgc
      421 gaccacccac gcgaacacgg cggtcggaag gacagacgcc gcggtgacca gagaggtgat
      481 tgcgaggacc aggaaaaccg caggcaggat cggcatcgtg ggaatcgaca ccgcgcgcgg
      541 cctgaacccc ctgatgccgg caaacacacc gtaggcgagt gccgggaaca cgtataccgg
      601 agtatgcgtc acgatctgaa ccaaagcttc cataacaacc ctcctcatcc aaaaaaggat
      661 gatcagagca tcgcgaatca gcgggtcacg agaacagtgc cgaaagtcat ggaaaagtca
      721 cggtgcacgg gcgccgcggc cggcgggccg ccccgcgcaa ggggaggttc cgggcaagaa
      781 aaaagcatgc ggggaattga gccgccccgg actcgaaccg gggacccaca gcttaaaagg
      841 ctgttgctct gccaactgag ctagcggccc ggtagagccg gtactgtacg cgccgcgtat
      901 cggcttgtca attccggccg atgccaccga tggcaccgac cgaccgcgcg gctcgacgga
      961 gccctcccgc ctccaccgcg gtggggatcc ggttcagtat cggaactcga gcccgacgat
     1021 caggttgtag gccggccgca gtccgggctc cagcgagagc tccgaaccgt acatcgtcag
     1081 attgagtcgg aagacgctca ggttcagacc cgcacccgcg gagaagtatc cctcgttgaa
     1141 gccggcccga agggcaagga tctgcaagag ctggaactcg atacccagcc cgacgtgcag
     1201 gacgggattg gatgccgtgg tcgggtgggt gagaaagtcc aggatatcct gatagtccag
     1261 cagcaccttc agaccgccca gatacggctc cagcgcgccg aggttgggcg tgaaggcgaa
     1321 cccggccgcg aggtccaggg gcaccgtccc gaacgacatg gtgggcgcgg ctccctgacc
     1381 gaacgcggag aacgtggtgt aggagttgac cagcgccggc gcgtagaggt tccgtcccac
     1441 caccccgaag gagatcgtgt cgttccagga gtagcggatc ccgacgtcga ccccgattcc
     1501 cgcgtccagc tcgaagggct ggttatagat gatcgagacg tccggtgccg ccaggagcgt
     1561 gagaacgtcc aggatcgact cccggagcgt cacgtccgct cgggcaaagg ccttgagggt
     1621 tgcgccgatg tcgagcttgc ttttccacga cgccggcagc gggatggcaa acgcgtagcc
     1681 gcccgcaaag atgacatcct ctccgatgct cgcggtgacc gtgggcgggg tccctacggt
     1741 ggtgaaggtc acgttggacg cgttgaagat cccgaagccg agtccgccgc ccacgtaacc
     1801 gagcgccagc ggtccgttga gcgtcccgga ggcaaaaaga ctgctcagca gcgagatcac
     1861 cgacggggtc tggagcagag tggtgaagtc cgttcccgtc gtgcccttgt agacgaggtc
     1921 ggcgatgctg aagaccggcc ccgtcagatg cagcgtgacc tcggacacgg aaagctgagg
     1981 cccggccagc gcgaatccgg ctggattgga gaagaggctt cccaggtcat cggccagcgc
     2041 cgcgtgcaaa ccacccaagg ccgaggcgcg agcggagggg atctccaccg gcgcgacgac
     2101 cgtggtctgg ccaaaaaccg gtgccgtcag gcccaggacc aggaggaaag caagaaagac
     2161 tctgcggggt ctgttcacgt tctctctcct tcaggcccct agagacccga gaggtagctg
     2221 gacagcaggt tggcgtacgg gtccgtcgtg ctgatcggcg cgaggaggtt cacggcgggg
     2281 gcaacgtccg acgcggtgag gctggtgagg ttggtggagg ggccggccgc ctggagaacc
     2341 aggccaacgg cccccatcat ccggtcattc gtggtgaggg ggtaggactg ggtattcagc
     2401 gtcgagagcc gggtccccgc atccttcagg tagctcgtat cgatgcccgc cgtggcggcc
     2461 acgaaatccc ctatggcgtt gcccgacagg gaaagctggc cggtagcaac ctgggtcaga
     2521 agggtgccta ccccggagag ctccacgccg agttgggccg cctggtacgt cgcgtccggg
     2581 ctcgtgtcgc tcttgatggc gtcgtaggct gccttcatgg ccgacgggtc tccgctctga
     2641 agcgcttcct tcgctgcttc caccttctga gcggggctca aggtggaagg atcacgccga
     2701 aggaatgcga gtggtgtgta ggtaaagatc tcctggcacc cgacgagggt gaggagagag
     2761 aagacgatca cgagggccga tcggacggcc aaccctcttc cgttcatggc atcgcctcct
     2821 accgcggcgc atgggccgcg tcgacggggc gccgtttggc gctcgccgca agacggtcag
     2881 agccggatcg agacatcgag cgtggccccg gctcgcggcc agtcctgaac ataatacccc
     2941 agctcctggg taaagacagc gaagctcacg tccagcagcg ggatcttcag accgccgccg
     3001 gccgtgaagt aaccctggtt gacgccagct tgcacgaaga acagggaaag gagtctggcc
     3061 tgcccgccga cgtgcagcgc ggtccagacg gttcgggccc ccgagatgag acccgtcagg
     3121 tcgtcgaggt ccaggctcag ggacggatcg atccagccgc tgaacctgcc gagatccgga
     3181 tgcagcgtca cgccgacgcc caggtccatc gggatcacat agctgtccga caggcttgcc
     3241 gttcccgccg gcagcttccc ctggctgctg aagctctgct cgagggaagc cagcgcgttc
     3301 tcgctgtagc ggaactgcgt gccgcccaga tcgcggcagg cgatgccgaa cgtgaaccag
     3361 cctgcctcga ggatcgtgcc cacatcgact ccgatgccga ccccgtagag agcgctggcc
     3421 gagttgaggg ccgcgaacac gtcacctccc gacgcgatgg ctccgagtgc ccccagggtg
     3481 gcggcgttgt ccatgagcac gtggatccgg atcatcggcc ggacggctcc tccgacgtgc
     3541 acggtaagcc cgggcgccag ggacagaggg accgacattc ctccgatgaa cccgatggtg
     3601 cccgtgaggt ctcccgtcgc gcccagcagg gtgggtccgt gagcgagcga atccacgatc
     3661 aagcccaccc ccaaccccag gccgttccct acgtagccag tgccaacgga tgagccgagg
     3721 ccgaagccgc ctcccgtgac ctgggaggcg aggaacgacg cgtagtcggc cgggctcggg
     3781 cgcgtggaga gcgctttcac gcccatggac agcgcctgat ccggccgcag gtaggcccag
     3841 agtcctgacg tggcaatggt cagcgagccg ttggaccggg agaagccggc cgggttcagg
     3901 aagaaggagt cccagccctg ggcgctggcc actccggttc ccccttgcgc aatgatggag
     3961 ggcgttatcg gggcgaaggg aatctcggcc acctgggcgg cgtaggccgt ccccacggcc
     4021 gtcaggagga tgagagcgac gagccgggct cggttgctca tggggactcc gttcccatca
     4081 gaacggtctc aattccgacg gcagtccggc gtggacgatg ttcacgagcc acgtcggagg
     4141 cgaggcgtac gggtcaccga cggagtaccc cgccggaatc gtgctgggct tgccggccag
     4201 gacatcgtag atctggggga cagcctgcga cgagccaacg agcccgctga cgctcgccac
     4261 gacgaaggag gccacgtgcg ccgtcagagc cttctgggcc acgtccccca tgtccacgcc
     4321 gccggggatc ggaggtatgc tg
//
LOCUS       DASRZS010000032         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2324741, whole genome shotgun sequence.
ACCESSION   DASRZS010000032 DASRZS010000000
VERSION     DASRZS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2324741"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFH83_00845"
     CDS             complement(<1..322)
                     /locus_tag="VFH83_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HET6484932.1"
                     /translation="MMRSLWTAASGMEGQQFNIDTISNNLANVNTTGYKEMRADFEDL
                     LYQTLKLAGTPATEATVVPTGVQVGHGVKPGATQKIYTQGALQSTGNVSDMAIEGEGF
                     FRVQQ"
     gene            complement(319..1167)
                     /locus_tag="VFH83_00850"
     CDS             complement(319..1167)
                     /locus_tag="VFH83_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014489217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HET6484933.1"
                     /translation="MVRGLFTGASGMVVQMDRMDEIANNLANVDLTGYKKDTAIQKAF
                     PEMLMHRLNDDGVHVFPVGSVDTTPLVGKLGTGVETNEVFTNFSQGAMKQTENDFDMA
                     LEGPGFLVVQTPLGERMTRNGSFVLNSDGYLVTSSGDYVMGENGPIKLKKNNFVVDQD
                     GVVWQNATFAADDKRLVSQAENTWDNLQRVDRLQIVDVKRDRYLQKQGNSYWQSTDAS
                     GPPDILPAGARPKVRQGFLEGSNVNPVQEMVEMIEVNRAYDANQKTVQTEDALIGKLW
                     NEVVKV"
     gene            1304..1376
                     /locus_tag="VFH83_00855"
     tRNA            1304..1376
                     /locus_tag="VFH83_00855"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1337..1339,aa:Thr,seq:cgt)
     gene            1534..1929
                     /locus_tag="VFH83_00860"
     CDS             1534..1929
                     /locus_tag="VFH83_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484934.1"
                     /translation="MLRSFLKNRVVIAVILCLQVIPLLLFPASAYSLSTQEWWLPILL
                     VVLVAVSLVQLIGRRSLAAWPWHLLNFAQGLNIISRLMMVLPHTVVYGEAGARFNTGY
                     VVLSVLAMLLSVFEIWYCDLPDVHNRLLA"
BASE COUNT          344 a          755 c          674 g          504 t
ORIGIN      
        1 attgctggac ccggaagaac ccctcccctt cgatggccat gtccgagacg ttgccggtgg
       61 actgcagggc gccctgcgtg tagatctttt gcgtggctcc tggtttgacg ccgtgaccga
      121 cctgcactcc ggtggggacc acggtggcct cggttgccgg cgttcccgcc agcttcagcg
      181 tctggtaaag caggtcctcg aagtcggccc gcatctcctt gtacccggtg gtgttcacgt
      241 tggccaggtt gttggagatc gtatcgatgt tgaactgctg gccctccatg ccggaggccg
      301 cggtccacag agatcgcatc ataccttcac cacctcattc cacagcttgc cgatcagcgc
      361 gtcctcggtc tggacggtct tctggttggc gtcgtaggcc cggttcacct cgatcatctc
      421 caccatttcc tggacggggt tgacgttgga gccttccagg aagccctggc gcaccttggg
      481 gcgcgccccc gccggcagga tgtccggcgg ccccgaggcg tcggtgctct gccagtagga
      541 gttgccctgc ttctgcaggt agcggtcccg cttgacgtcc acgatctgca gccgatccac
      601 ccgctggagg ttgtcccacg tgttctccgc ctgagagacc aggcgcttgt cgtccgcggc
      661 aaaggtggcg ttctgccaca ccacgccgtc ctggtccacc acgaagttgt tcttcttgag
      721 cttgatgggc ccgttctccc ccatcacgta gtccccggag ctggtcacca ggtagccgtc
      781 gctgttcagg acgaaggacc cgtttcgggt catccgctcc cccagcggcg tctggaccac
      841 gaggaagccc ggcccttcga gcgccatgtc gaagtcgttc tccgtctgct tcatcgctcc
      901 ctgggagaag ttcgtgaaca cctcgttggt ctcgacaccg gtgccaagct tcccgaccag
      961 gggcgtagtg tccaccgacc cgaccgggaa cacgtgcacc ccgtcatcgt tcagtcgatg
     1021 catgagcatc tccgggaacg ccttctggat ggcggtgtcc ttcttgtagc ccgtgaggtc
     1081 cacgttggcc aggttgttgg cgatctcgtc catgcgatcc atctggacga ccatgccgct
     1141 cgcaccggtg aagagccctc gaaccacctg ctacctccag accggccgcg gcccccgcgg
     1201 cctgcggggg ccggggagcc ggcccctgat cttctcttac cattatcgga gaccggcgag
     1261 gttccttgac gtgaccgtcc cgttcggcta tcctgcgcat cgcgccaaga tagctcagcc
     1321 ggtagagcag cgcactcgta atgcgcaggt cctcggttca attccgagtc ttggctgtag
     1381 acagggatgt cgaagccgag cgagcacagg atgtgcggaa gcgaggctgt aggcatggat
     1441 gccgtaacct gcccgaggca tggatgccga aagggtgggt gttttttcct cttccccctc
     1501 gacagtagaa gcgcatccat ggtaccgtgc gccatgctgc gctcatttct caagaaccga
     1561 gtggtgatcg ctgtcatcct ctgtctccag gtcatcccgc tgctgttgtt tcccgcctcc
     1621 gcctactccc tgtccaccca ggagtggtgg ttgcccatcc tcctggtggt cctggtagcg
     1681 gtctccctgg tgcaactcat cggacgccgt tccctggctg cctggccgtg gcacctcctg
     1741 aacttcgccc aggggctgaa catcatcagc cggctgatga tggtgctgcc ccacaccgtg
     1801 gtgtacggcg aggcgggtgc ccgcttcaac acggggtatg tcgtgctctc ggtgctggcg
     1861 atgctcttgt cggttttcga gatctggtac tgtgacctgc cggacgtcca caaccggctg
     1921 ctcgcgtagc gctcgtgctt gacattttat tcgcccagga tatgattttt cagcttccat
     1981 ccaaagaggc gcacgcccgc gcgcggtgcg ggtgctctcc ggcaaaagga ggtgagatcg
     2041 tcccagcatg gactccgcat atctgacggc ccgacgagcc ctctcctttt ccgtactgcg
     2101 agcaagcgct ctcacacaac tttcgaagga ggtttgaatg aagcgtttcc tgtccatctc
     2161 tgccgtggtg ctgctgctgg ccctcgcgct gatcggctgc cagcaggcac ccaaggagct
     2221 gaagatcgcc atcctcgcgc ccctctcggg ctcgaacccg accttcggcg tctctac
//
LOCUS       DASRZS010000033         2962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3096632, whole genome shotgun sequence.
ACCESSION   DASRZS010000033 DASRZS010000000
VERSION     DASRZS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3096632"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VFH83_00865"
     CDS             <1..984
                     /locus_tag="VFH83_00865"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET6484935.1"
                     /translation="SNAKGFLQTIGFLLVIAATGCVSYLCFQLHRWVLLALCLYVHGT
                     FYGFFGAGQHELSHNTVFSSRWLNIAVTALFGLLYWPMNPHFYRLSHQKYHHRYTLYQ
                     RSDGEDVANYMELKPLFIFGLFFYLIAPKALAQCLYRLFTLTPISRGWRGRNFSLDTW
                     EQFIWKNATEAEKRQVRRLHVGALVVQVVFVAACITVGVMTHTGVWFLPILVTLAPFY
                     GGGFLGFMTGIHQHAGREVNNPDFRISCGSVNVGPLNSLLYWHMENHTEHHMFAAIPC
                     YNLKPFSRFVADQMPPRTYTIPRLLELNRICREKWGSYKNWRDTLGLYKGL"
     gene            complement(1018..2772)
                     /locus_tag="VFH83_00870"
     CDS             complement(1018..2772)
                     /locus_tag="VFH83_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484936.1"
                     /translation="MESGAHPFTDRVAVLGFPDSTTRYHAHAPAPWSRETLRKVKRLG
                     FTAVQLNLAWGARPDDDPLNLEDVVELDPAEAARLPQVVPLNCKPGSEARERRRRNLR
                     DRLGLATAEGMRTIFHFGAPYNAHARYGDGPPNCLLDPAVHQRYEGLLRAFARDFPGV
                     DDLWVYTYDQDAWLCSEFGPCPRCLGVPLHERLVPFLDRLRQTWLEINPSGRVWWEPW
                     ELSAGQVYACLERLPDRGMGFAAHANIAESMSAVATDRWLENAAEIARQRGVPVTVEW
                     FLGGLSEEVEPLSRLTHPVLIWRGLSRIAGLPGVGGIKEYYGILPDREDPNLRMTSIF
                     LAEPSLSEADALRRLARPYGAAGPAVIRFWQAASVAMARYPWDLSWMARQVGRSATDH
                     SLHAAIIRPMLCPTPSWQSTRGTVFIRTEDTDSHPWLLEDAQLRFQQAAACWSAAVRL
                     AEEAEPAVPPELKPDLRACRDDTDRIRRRALAYSCHLRATCISLSLRRRQQQGLPLPP
                     SSLKELDAVLRESRANHAEECAASAPVGRGSVAGTAWPEMDQALADLARDPREFLGRW
                     LTAQPDRASRGTFSLTSV"
BASE COUNT          486 a         1026 c          967 g          483 t
ORIGIN      
        1 agcaacgcca aggggttcct gcagaccatc gggttcctcc tggtgatcgc ggccacgggc
       61 tgcgtcagct atctctgctt ccaactccat cggtgggtgc tcctcgccct gtgcctctac
      121 gtgcatggga cgttctacgg cttcttcgga gcgggccagc acgagctgag ccacaacacc
      181 gtgttctcga gtcggtggct gaacatcgcc gtgacggcgc tctttggcct cctgtactgg
      241 cccatgaacc ctcacttcta ccggctgagc catcagaagt accaccaccg ctacacgctg
      301 taccagcgga gcgatgggga ggacgtggcc aactacatgg agctgaagcc gctgttcatc
      361 ttcggcctgt tcttctacct gatcgcgccc aaggcgctcg cgcagtgcct ctaccgacta
      421 ttcaccctga cgcccatcag ccggggatgg cgcgggcgca acttctcgct ggacacctgg
      481 gagcagttca tctggaagaa cgcgacggag gccgagaagc gccaggtccg caggcttcac
      541 gtcggcgccc tggtggtcca ggtggtcttc gtcgcggcgt gcatcaccgt gggagtcatg
      601 acccataccg gcgtgtggtt cctcccgatc ctggtcaccc tcgcgccgtt ctacggcggc
      661 gggttcctcg gcttcatgac gggcatccac cagcacgccg gccgtgaggt gaacaacccg
      721 gacttccgga tctcctgcgg cagcgtgaac gtcggccccc tcaactccct cctgtactgg
      781 cacatggaga accataccga gcaccacatg ttcgctgcga tcccctgcta caacctcaag
      841 ccgttcagcc ggttcgtggc ggaccagatg ccgcctcgga cgtacacgat cccgcgcctg
      901 ctcgagctga accggatctg ccgggagaag tggggatcgt acaagaactg gcgcgacacg
      961 ctggggctct acaagggtct gtagggccgg gtcgccacgg ctgggccggc gacggcccta
     1021 caccgaggta aggctgaagg tgccgcgcga tgcccggtcc ggctgcgccg tcagccagcg
     1081 ccccagaaac tccctgggat ctcgagccag atcggccagc gcctggtcca tctcaggcca
     1141 cgcggtcccg gcgacggaac cacgcccgac gggcgcggag gctgcgcact cctcggcatg
     1201 gttggcgcgg gactcccgca gcaccgcgtc cagctccttc agactggacg gtggcagcgg
     1261 gagtccctgc tgctggcgtc tgcgaaggga cagggagatg cacgttgccc gcagatgaca
     1321 cgagtacgcc agcgcccgcc ggcgaatccg gtccgtgtcg tctcgacagg cacggaggtc
     1381 cggcttcagc tccggcggga cggcgggctc cgcctcttcc gccaatcgga cggcggcgct
     1441 ccaacaggcg gcggcctgct ggaatcgaag ctgcgcgtcc tcgagaagcc acgggtggga
     1501 gtcggtgtcc tccgtgcgga tgaacaccgt gccgcgcgtg gactgccagg agggcgtggg
     1561 acacagcatg ggacggatga tcgcggcatg gaggctatgg tccgtcgcgc ttcttcccac
     1621 ctgccgcgcc atccaggaca agtcccaggg gtagcgcgcc atggcgacac tggcggcctg
     1681 ccagaatcgg atcacggcgg gacccgcggc gccgtagggc cgcgccaggc gacggagcgc
     1741 gtccgcttcc gacaggctcg gctccgccag gaaaatcgac gtcatcctga ggttgggatc
     1801 ctcccggtcc ggcaggatcc cgtagtattc cttgatcccg cccacgcccg gcagtccggc
     1861 gattcggctc aggccccgcc agatcaggac cggatgggtc aggcgggaga gcggctcgac
     1921 ctcctcgctc agaccgccga ggaaccactc cacggtcacg ggaaccccgc gctgccgagc
     1981 gatctccgcc gcgttctcca gccatcggtc ggttgccacc gcggacatgc tctccgcgat
     2041 gttggcatgg gccgcgaatc ccatgcctcg gtccggcagc cgctccaggc aggcatagac
     2101 ctgtccggcg gagagctccc atggctccca ccagacccgg ccggacgggt tgatctccag
     2161 ccacgtctgg cgcagccgat ccaggaacgg gaccagccgc tcatgcagcg gcaccccgag
     2221 acaccgggga cacggtccga actcgctgca cagccaggcg tcctggtcgt acgtgtagac
     2281 ccagaggtca tcgacgccgg ggaagtcccg ggcaaatgcc cgaaggaggc cctcgtaccg
     2341 ctggtgcacg gcagggtcca gcaggcagtt cggcggaccg tcgccgtagc gggcgtgggc
     2401 gttgtacggc gcgccgaagt ggaagatcgt ccgcatcccc tcggcggtgg ccaggcccag
     2461 ccgatcccga aggttgcgac gtcgcctttc gcgcgcctct gaacccggct tgcagttcag
     2521 gggcaccacc tggggcaacc gcgcggcctc cgctgggtcc aactccacga cgtcctccag
     2581 gttgagcgga tcatcgtcgg gcctcgcgcc ccacgccagg ttgagctgga ccgcggtgaa
     2641 cccgagcctc ttcaccttgc ggagcgtctc acgggaccag ggagcggggg catgggcatg
     2701 gtaccgcgtg gtcgagtccg ggaacccgag caccgcaact cgatcggtga acggatgcgc
     2761 gccgctttcc atcgttccgc ctctcccttt caatcgaagg tgtctacctc gaacgaggtg
     2821 agcccgaaat ccacgttggt gttcgagacg ccgaagttga acccgatccc ggggctgccg
     2881 gtgtcgaagg tgtcgtcggt tgcggagacc acctccaggc cattgacgaa accgcggatc
     2941 acggtgccgt cgatgctcgc ct
//
LOCUS       DASRZS010000034         6933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3481269, whole genome shotgun sequence.
ACCESSION   DASRZS010000034 DASRZS010000000
VERSION     DASRZS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3481269"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFH83_00875"
     CDS             <1..568
                     /locus_tag="VFH83_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484937.1"
                     /translation="FYDHPEAVRTAIDFISETFLEMQEDLWRLIQPTNQRGGVLPWMM
                     LWMPGKHGNQLACDLSSVLSPSIFRSFFIPSIRREGQWTAFGTYHLDGPACIRAHLDA
                     LLETDEIKAIEWTPGAGSPPASHPEYFPQYRRIQASGKRLILLAEIREVETILSELSA
                     RGLYIITRADTEEEAKDLLRKVGSWTRS"
     gene            647..1660
                     /locus_tag="VFH83_00880"
     CDS             647..1660
                     /locus_tag="VFH83_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET6484938.1"
                     /translation="MALQGREQAGPPADPEGDTLSRVKTALLSMQRYSWEQGVAAQAF
                     LELGEADLVVLMAREAVNRQLADGRLAVMGNLSAVTDPAAIGEAVLFTAKRTGDLRIT
                     EAAERLLHWLLRAAPRTSSGVLYHLHDRPQVWVDSLYMAPPFLSAAGHHDEALKQIAG
                     MRTLLLDSRKRLLSHIWDEGTGSFGRKAFWGVGNGWALAGMTRVIRTLPDAFRNERET
                     LVSWVREGVDASLPFLRQDGLFHDVVDDPSTFVETNFAQMLSYVLYRGMAAGWLGAGY
                     RQSADAMRRAAHAKVDSSGLVQGVCASPYFDRPGTAPEGQAFFLLMEAAERDFQRGED
                     PTR"
     gene            1861..2778
                     /locus_tag="VFH83_00885"
     CDS             1861..2778
                     /locus_tag="VFH83_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003727233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6484939.1"
                     /translation="MKQITIGRGELSGSEIALGCMRMSGLAAKDAAGVVHAALEAGIT
                     YFDHADVYGGGKSEEVFAQAIGMSPAARAGIVLQSKCGIGKGMYDFSKDHIVSAVQGT
                     LKRLRTEYLDVLLLHRPDTLMEPQEVAEAFTALHRSGQVRHFGVSNFNPMQIELLSRT
                     VPYRLIIDQLQLSLTETGMIDSGINVNTHFDGSVDRDGGILEYCRLQQITIQAWSPLL
                     HGWFEGPFLGSEKFPALNAAIDSVAKAHGIPPVAVAIAWILRHPARIQPVVGTMNPGR
                     LGDVCKASDVGLSRKEWYDLYLAGGKKLP"
     gene            2864..3997
                     /locus_tag="VFH83_00890"
     CDS             2864..3997
                     /locus_tag="VFH83_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6484940.1"
                     /translation="MSGKVKVAVVGLGFGAEFIPLYQRHPDAECYAICQRDEENLKRV
                     GDRFRVERRFTRFEDLLRVREIDAIHIVTPIAAHGPMTIAALEAGKHACSTVPMATTV
                     KECLQIAEARRKSGKVYMMMETAVYTREFLYVKEMADNGELGTVQFLRGSHQQNMSLP
                     GWPEYWYGFPPMHYATHAVSPLMALIDGEVESVVCFGSGTIREEYAKRYGSAFAIETS
                     LMRVRGSDLACEVTRSLFDTIRQYRESFDVYGTRLSFEWEQLAGEKPVLFSGFEDARR
                     VEVPDFGSRLPKEIAPYTIHGVYDEQHEHTSFVQGAGHGGSHPHLAHEFVRAIVEGRE
                     SAVDAWRAANWTMAGICAHESAMKGGARIDVPRTGLQGAGTIS"
     gene            complement(4387..4692)
                     /locus_tag="VFH83_00895"
     CDS             complement(4387..4692)
                     /locus_tag="VFH83_00895"
                     /inference="COORDINATES: protein motif:HMM:NF042532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protealysin inhibitor emfourin"
                     /protein_id="HET6484941.1"
                     /translation="MLISVTRTGGFAGLSETLIRADTETLNTQVAASLRDMVRAMDFF
                     SLPSRVGAEQMGADLFIYEVTVTDGEQSHTVAFADARTPENAPLHDLLGALGGGGPQ"
     gene            complement(4762..6168)
                     /locus_tag="VFH83_00900"
     CDS             complement(4762..6168)
                     /locus_tag="VFH83_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484942.1"
                     /translation="MEDKTMTLPVSFVPTPTKEFRLHRKTVKTTLEPGRPTTLTPIPI
                     PKPIPGPIPGPIPIPFAGSRVLIYKQDPSVTEIGVRKAYLPGAVLNGPRDAYITSGTP
                     GLPVISANVFGDFIEPPNTDSFDAVHTFSIVRMVLTMYQRALQPTAVRWEWNGNGNGD
                     PINVFPHAGVTMNAYYSRNDKALKFFYFTKPGDPPGSPLIYTCRSLDIVAHETGHAVL
                     DGLKPLWIGAAGNPPQTGGLHESFGDLTSIFLALSQLDQVEAIIAQTKARLHDKTFLS
                     DLAEQFGLALGRPNGLRNADNDLKLSEVGTEVHALSQVFTGAVYDILADIFAFERNPA
                     ARDDAIVLYEAGRYLMSLVLRALLAAPAQAATYADVANAMLTIAARDGRPVQYRNFIR
                     NRFTVREVVVSPTPLTEDVPEGARLAANITDAPDSEQDRRACCGTLQLPEYSGVEQIV
                     LDEIESLRNLNGRGPKKK"
     gene            complement(6288..6752)
                     /locus_tag="VFH83_00905"
     CDS             complement(6288..6752)
                     /locus_tag="VFH83_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484943.1"
                     /translation="MDKLRPLVLSVLAIAAVAYVGGIVWAGVSSLRSATEPALPQIVT
                     QALTAIGGLLATHFGAVFGISLLPSGAPQRLLRGFPWAGHKRSAGASAAPLDWIQIAA
                     AYLYVSCLVVAVVFWAIDGFSSQTAQVLITMSSTLIGVVVGIGAVVLNVRQP"
BASE COUNT         1231 a         2143 c         2314 g         1245 t
ORIGIN      
        1 tttctatgac cacccggagg cggtccgcac ggcgatcgat ttcatcagcg agacatttct
       61 cgagatgcag gaggatctgt ggcgactcat ccaaccgacc aaccagcgcg gcggcgtgct
      121 tccctggatg atgttgtgga tgccggggaa gcacggcaac cagctcgcgt gtgatctgtc
      181 gtcggttctc tcgccgtcga tctttcggtc tttcttcatt ccctcgatcc ggcgcgaagg
      241 ccagtggacc gcgttcggga cctaccacct ggacggaccg gcgtgtatcc gcgcccatct
      301 cgacgcgctc ctggagaccg acgagatcaa ggcaatcgag tggacgccgg gcgccgggtc
      361 tccgcctgcc agccatcccg agtatttccc tcagtataga aggatccagg cgagcggcaa
      421 aagactgatc ctgcttgcag agatccgaga ggtcgaaacg atcctctcgg agctgtccgc
      481 tcggggcctg tacatcatca cgcgcgcgga cacggaggaa gaggcgaaag acctgctgcg
      541 aaaggtcgga tcatggactc gatcgtaacc cggcgggttg cacgcggctt acggacgccc
      601 cgcctggcgg gtcccacgag gtgcgccgac aacaagggag gccagaatgg ctctacaggg
      661 ccgtgagcag gcgggtcctc ctgcggaccc cgagggagac acgctgtcga gagtgaagac
      721 ggcgctgctc tccatgcaac gctactcctg ggagcagggc gtagccgcgc aagcgttcct
      781 ggagctcggt gaggccgacc tcgtggtcct catggcgcgg gaagcggtga accgacagtt
      841 ggccgatggg cggctggcgg tgatgggaaa cctgtcggcg gtgaccgacc ccgcggcgat
      901 tggggaagcg gttctcttca cggcaaagag aaccggcgac ctccggatca cggaagcggc
      961 agagaggctt ctccattggc tcctgcgggc ggcgccgagg acgtcgagcg gcgtcctcta
     1021 tcacctccac gatcgaccgc aggtatgggt ggattccctt tacatggcgc cgccgttcct
     1081 ctccgcggcc ggccaccacg atgaagccct caagcagatc gccgggatgc gtacgttgct
     1141 tctcgactcc cgcaagagac tcctctcgca tatctgggac gaagggaccg gttccttcgg
     1201 gcgaaaggcc ttctggggcg ttggcaacgg gtgggcgctt gcgggcatga cgcgggtcat
     1261 tcgtaccctc cccgacgcgt ttcggaacga gcgggagacg cttgtctcct gggtgcggga
     1321 aggggtggat gcaagcctac cgttcctgag gcaggatggt ctctttcatg acgtcgtcga
     1381 cgacccgtcc acgttcgtcg agacgaactt cgcccagatg ttgagctacg tgctctaccg
     1441 ggggatggca gcgggttggc ttggtgccgg ctaccggcaa agcgcagacg ccatgaggcg
     1501 agccgcccat gcgaaggtgg attcctctgg cctggtgcag ggggtctgtg cttccccgta
     1561 cttcgaccgc cctggcaccg cgccggaggg acaggcgttc ttcctcctca tggaggcagc
     1621 cgagcgggac tttcagcggg gtgaggatcc gacccggtag actccaccat cccgcgttgt
     1681 ctgtcgcggt cagcccagac gccgcgatag tgcactccgc gatatcccac tgggatgtgg
     1741 ccgcgaacaa ctaggctccc cttgccgcgc tggccgtcga ggcagccggg cgccgagtac
     1801 gaacggcatg gcgctgacga aacgtcacgc agtctggtac catccggctg gaggaacgag
     1861 atgaaacaga tcacgatcgg ccggggtgag ctcagcggtt cggagatcgc ccttggatgc
     1921 atgaggatga gcggcctggc ggcaaaggat gccgccggcg tcgttcacgc cgcgctggag
     1981 gcgggcataa cctacttcga tcacgccgat gtctacggag gcggcaaatc ggaggaagtc
     2041 ttcgcccagg ccatcggcat gtccccggcg gcgcgagcgg ggatcgtcct ccagtccaag
     2101 tgcggcatcg gcaagggcat gtacgacttt tcgaaagacc acatcgtctc ggcggtgcag
     2161 ggcacgctga agcgccttcg gaccgagtac ctggacgtgt tgctcttgca ccggccggac
     2221 accctgatgg agccccaaga ggtggctgaa gcgttcacgg ccctgcaccg gtccgggcag
     2281 gtccgccatt tcggcgtgag caacttcaac ccgatgcaga tcgagctcct gagccggacc
     2341 gttccctatc ggctcatcat cgaccagctc cagctcagcc ttacggagac gggcatgatc
     2401 gattccggca tcaacgtgaa tacgcatttc gatggatccg tggaccggga cggcggtatc
     2461 ctggaatact gccggctcca gcagatcacc atccaggcgt ggtcacccct tctgcacggc
     2521 tggttcgaag gacccttcct gggaagcgag aagttccccg cgctgaacgc cgccatcgat
     2581 tccgtggcga aggcgcatgg gattcccccc gtggccgtcg ccatcgcgtg gatcctgcgc
     2641 cacccggcgc gcatccagcc cgtggtaggg accatgaacc cgggccgtct gggggatgtc
     2701 tgcaaggcct ccgacgtcgg gctcagccgc aaggaatggt acgaccttta cctggccgga
     2761 gggaagaagt tgccctagga gttgggagcg ccgatattgg tcctgcgcgg ccgtggagcg
     2821 gttcgcgccg tcgcgaggcc cacggtggcc gagggaggat cccatgtccg gcaaggtgaa
     2881 ggtcgcggtc gtcggccttg ggttcggagc ggagttcatt cccctatacc agaggcaccc
     2941 ggacgcggag tgctacgcca tctgccagag agacgaggag aacctcaaga gggtcggtga
     3001 ccggttccgg gtggagcgcc gcttcacccg ttttgaagac ctcctgaggg tgagagagat
     3061 cgatgccatc cacatcgtca cacccattgc ggcgcacggc cccatgacca ttgccgccct
     3121 ggaggccggc aagcacgcct gcagcacggt gccgatggcg acgacggtga aggagtgcct
     3181 gcagatcgcg gaggcccgaa ggaagtcggg caaggtctac atgatgatgg agaccgccgt
     3241 atacacccgg gagttcctgt acgtgaaaga gatggcggac aacggggagc tcggaacggt
     3301 ccagtttcta cgcggctcgc atcagcagaa catgagcctt ccgggttggc cggagtactg
     3361 gtacggcttt cctccgatgc actacgccac ccacgcggtg agcccgctga tggccctgat
     3421 cgacggggag gtggaatccg tagtgtgttt cggatccggg accatccgag aggagtacgc
     3481 gaaacggtac ggatccgcct tcgccatcga gacgtcgctg atgagggtgc ggggatccga
     3541 ccttgcctgc gaagtcaccc ggtccctgtt cgacaccatc cgccagtacc gggagagttt
     3601 tgacgtctac gggacgagac tctccttcga gtgggagcag ctcgccggag agaagccggt
     3661 cctcttctcg ggattcgagg acgccaggcg cgtcgaggtt ccggatttcg ggagccgact
     3721 gcccaaggag atcgctccgt acaccatcca cggggtctac gacgagcagc atgagcacac
     3781 ctcctttgtc cagggcgcgg gacacggagg ttcgcaccct cacctggccc acgagttcgt
     3841 gcgcgccatc gtggagggta gggagtcagc ggtggacgcg tggcgcgcag ccaactggac
     3901 gatggccggc atctgcgccc acgaatccgc catgaagggc ggcgcgcgga tcgacgtccc
     3961 gcggaccggt cttcagggag cgggtacgat ctcctgaaag ctgccgtaaa tctgggtctg
     4021 gcccgcaagg cgaatgttcc aggtcgccgt gttggacgaa gggtgcacga tccggctgag
     4081 gacgggcagg ttgcgaggca cggcctggtc cgccctgcct ccacgaccgc gcagaagctc
     4141 gtatcggggc ggatgccttt tgtcggcagc ttcccgtccc atcttgcggg attcgtgttc
     4201 gcgatgagct tgaccttggc gaggttcttc tccgggtctt cgccgatctg gtgggagatg
     4261 agaaacgcct tcacgtggga atggaacaga cgatccatgg tagccccccc ggatccctag
     4321 tctacctctc cgcgtcgcca ccatccgacg tcctgccgaa cgcgcccagg cctcacggcg
     4381 ctgccgtcac tgaggcccgc ctcccccaag ggctcctaga aggtcgtgca ggggggcatt
     4441 ctcgggcgtg cgggcatctg cgaacgctac cgtgtgactc tgctcgccgt cggtcacggt
     4501 cacctcgtaa ataaacaggt ccgctcccat ctgctccgca ccgacgcgcg aagggagaga
     4561 gaagaagtcc atggcgcgga ccatgtcccg aaggctggcg gccacctggg tattcagggt
     4621 ttcggtgtcc gcccggatca gggtctcgct caggccagca aaacctcccg ttctggtaac
     4681 ggagatcagc atgcaggtac aactcctgga gggccccggg gagccgtctc ccgcaaggcc
     4741 ctcacggtgc atcatgcgcg gttacttctt cttggggcct cggccgttca ggttgcgcag
     4801 cgactcgatc tcatcgagca cgatctgctc cactcccgag tactctggca gctgaagggt
     4861 cccacagcaa gcccttcgat cctgctcgga gtcgggcgcg tcggtgatgt tcgccgccag
     4921 acgggcacct tccgggacat cctcggtgag aggcgtcggg gagaccacca cctctctcac
     4981 ggtgaatcgg ttgcggatga agttgcggta ctgcaccggt ctgccatcgc gcgccgcgat
     5041 ggtgagcatg gcgttggcca catcggcgta cgtggccgcc tgagccgggg cggccagcag
     5101 cgcgcgcagg accaggctca tcaggtaccg gccagcctcg tagaggacga tggcgtcatc
     5161 ccgtgcggcg gggttccgct cgaaggcgaa aatgtccgcg aggatgtcgt agacggcgcc
     5221 cgtgaagacc tgcgacagcg cgtggacctc cgtgccgacc tcgctgagct tcaggtcgtt
     5281 gtccgcgttg cgcagcccgt tcggtctccc cagggcaagc ccgaactgct cagccagatc
     5341 ggacaggaac gtcttgtcgt gcaggcgcgc cttggtctgc gcgatgatcg cctcgacctg
     5401 gtcgagttgg gagagcgcca ggaagatcga ggtgagatcc ccgaatgact cgtgcagccc
     5461 cccggtctgg ggaggattgc cggccgcgcc gatccaaagc ggcttcaggc cgtcgagcac
     5521 ggcgtgtccg gtctcatgcg ccacgatgtc cagggaccga cacgtgtaga tcagcggcga
     5581 tcccggcggg tcgcccggct tggtgaagta gaagaacttg agggccttgt cgttgcggga
     5641 atagtaggcg ttcatcgtga ccccggcgtg agggaacacg ttgatgggat caccgtttcc
     5701 gttgccgttc cactcccagc gaaccgcggt gggctggaga gcgcgctggt acatcgtcag
     5761 gaccatgcgt acgatcgaga aggtgtgcac cgcgtcaaag ctgtccgtgt tgggtggctc
     5821 tatgaaatcc ccgaatacgt tcgcggaaat gaccgggagt cccggcgtgc cgctggtgat
     5881 gtacgcgtcc cgcggcccgt tcaggacggc gcccgggagg tacgcctttc gcacaccgat
     5941 ctcggttacc gatgggtcct gcttgtagat gaggactcgg gatccggcga acgggatcgg
     6001 gatcggtcct ggtatgggac cggggatagg tttgggaatc gggattgggg tgagggtcgt
     6061 cggtctgccg ggctccaggg tggtcttcac ggtcttgcgg tgtagtcgaa actctttggt
     6121 tggcgtcggg acgaacgaca ccggtagggt catggtcttg tcttccatac ggattctcct
     6181 tgcctccttg acggaggcga aacacgatcg tcccaggtac tttctggact gttatctggt
     6241 gcacctcctc tctcctgccc gtggcaacat gcgccgcgat tcctccgcta tggctgtctc
     6301 acgttcagga caaccgctcc tatccccacg acgaccccga tgagcgttga cgacatggtg
     6361 atgagaacct gggcggtctg actggagaaa ccgtcgattg cccagaacac gacggcgaca
     6421 acgagacagg acacgtagag atacgcggcg gcgatctgga tccaatcgag aggcgccgct
     6481 gaggcgccgg cgctgcgttt gtgcccggcc cagggaaagc cccgcaggag ccgctgtgga
     6541 gcgccactgg gaagcagcga gatgccaaag accgccccga aatgtgttgc cagaagacct
     6601 ccgatggcgg taagggcctg agtcacgatc tggggcaggg ctggctccgt tgcgcttcgg
     6661 agactggaca cgcctgccca cacgatccca ccgacatacg ctacggcggc gatcgcaagt
     6721 acgctgagga ccaggggacg tagcttgtcc atggcacccc ctctcaggaa cggcgcgtga
     6781 tgcgcctcat tcaccgctca ccgatgcctt gagcgatcac cccgaggagc atgccctcct
     6841 cgaaggtgac ctggggcgct gatcgcagct tcgccgccaa gtcgctggtg attcccgtcg
     6901 agaactccga ccactgggcg ctggtgagcg ggt
//
LOCUS       DASRZS010000035         6094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3866956, whole genome shotgun sequence.
ACCESSION   DASRZS010000035 DASRZS010000000
VERSION     DASRZS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3866956"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..874
                     /locus_tag="VFH83_00910"
     CDS             77..874
                     /locus_tag="VFH83_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET6484944.1"
                     /translation="MVAYLVDTHAHLSDSSFDADREAVLRRASAAGIAAVLTMAESPE
                     DCKRCLSLATEFPQVLAAAGLHPQAAEAKAADDVVALIRDASDLVAIGEVGLDFWIAQ
                     DEAGRQIQREVFERLTRLSVELGLPLNVHSRSAGRQAIELLMRLGARKVQMHAFDGRA
                     STAMPAAEAGFFFSIPPSIVRSRQKQKLVERLPLSCLLLESDSPALGPDPTGRNEPAN
                     LQVAVRAIAEIKKVPEAEVRAATWENAVRLYGARLEAAAMAAHQRSP"
     gene            923..1390
                     /locus_tag="VFH83_00915"
     CDS             923..1390
                     /locus_tag="VFH83_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484945.1"
                     /translation="MKSLALAASFLFVSLSALAFDFRSVSWMMSRDQVVAAENLSIVS
                     ENGFGGQQELSYQTSLNGFAGSLSYTLENDRLVAASFSFPTDNHGELFRYLKGTLIRQ
                     YGPPSFQSDRLIGWRMNRTEIALAFLSGRICHLSYWEKSYFARINNLSVSASR"
     gene            1505..2263
                     /locus_tag="VFH83_00920"
     CDS             1505..2263
                     /locus_tag="VFH83_00920"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484946.1"
                     /translation="MRVSALRVAVIAVALIAGAGLVWGQSATTIQVKTGPNGVGYLTD
                     SKGMTLYYYTKDADGQSACYGGCAKAWPVFYTSTVTIPPSLDASDFGTITRTDGTMQT
                     TYYGWPLYYWQNDKSPGDMTGEGVGKVWYIMKSPWYSVTVSTNAQLGNYLVDENGMAL
                     YWFTKDSVGQSACMGDCLKAWPAFTATSIVVPSALSVSDFGTIIRADGTIQITYKGYP
                     LYYWVNDKKRGDVTGQNVGKVWFVIDPEKFPPKM"
     gene            2408..3265
                     /locus_tag="VFH83_00925"
     CDS             2408..3265
                     /locus_tag="VFH83_00925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET6484947.1"
                     /translation="MSAKKGSKPQGRATHAGSPRSRHPPGADVFFETPGLRIIHNDLL
                     KTVSVPARSVDLIVTSPPYNVDIHYQSHVDRLGYEEYLEFSRTWLARCLRWLKPDGRL
                     CLNIPLDKNKGGQQSVGADLTSLAKRAGYQYHSTIVWNEGNISRRTAWGSWMSASAPY
                     VIAPVELIVVLYKDVWKKTRAEARSDTTRDEFMGWTNGLWTFNGENRKRANHPAPFPV
                     ELPRRCIKLFSFVGDTVLDPFLGSGTTLVAAAANGRFGIGIEIDESYCRLARERLARE
                     TAVHRGETP"
     gene            3262..4146
                     /locus_tag="VFH83_00930"
     CDS             3262..4146
                     /locus_tag="VFH83_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET6484948.1"
                     /translation="MTGLAEWLKGRQPLLLDGAMGTELDRRGVEGRCLCNLSGPRHVI
                     AVHRDYREAGSHAVITNTLTMNRSFIESHRIGVDVLEVNRAGAALAREAAGPGGLVLG
                     NLSSTGQLLEPYGPVPAAVVLEAFREQARVLAEGGVDGFIIETMIDLREAECALKACK
                     EVSGLPVIVCIAFDTAQRGGRTVMGNSAEQCVRSLTEAGADAVGANCGGVDPAQMAEI
                     VSRMVSISTVPVAAEPNAGLPKLVGGKTVFDMEPRSFALGLAACAKAGATILGGCCGT
                     TPDHIRAFAALGAEPWLV"
     gene            4134..5081
                     /locus_tag="VFH83_00935"
     CDS             4134..5081
                     /locus_tag="VFH83_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6484949.1"
                     /translation="MACLIVPMAQGHVPQAAELFRGAYQRERRCSPLLPPEPLGGEGT
                     LREHLLVDLANPGVVAVDGGRLIGYLCVSARFPFKGLSAALVREHSHSAAPGQEPSLL
                     PELYAALGETLIEQGVQLHIVAHLASDGELKETLFQLGFGAFLTERLRDLSPVEGAAL
                     APIEPLRDPGELVALEMEHRRYYLQSPIFLTKDDTREAALAGLCEDRDSGRTALVYRD
                     HGTPAAYMVVGPCSAEQEGRLLRGSKTAQVCSAYATPSVRGRGIGRALLQGCVDWARC
                     AGYERLFVEHETANLAGRRFWGRHFAPYLHFSMRYVEGR"
     gene            5092..5631
                     /locus_tag="VFH83_00940"
     CDS             5092..5631
                     /locus_tag="VFH83_00940"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6484950.1"
                     /translation="MDFSRVTTPQRVSLLISFLDIQGFLGISQKLNDPLDLFALLDDL
                     AKMIIHDVDASSGRVIKLIGDSALVVYPEEAIDRGVNALLALKTRLEEDLSRRGFSNR
                     LRMTAHFGEAAIGLLGEGQCRHLDVLGDSVNTAAMLERTGHGGRLILSPQAFRRLSSS
                     TRKLFHRFTPPQVYLAEER"
     gene            5638..>6094
                     /locus_tag="VFH83_00945"
     CDS             5638..>6094
                     /locus_tag="VFH83_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484951.1"
                     /translation="MTERIRWSPRIHPDLVRRLYEADGRGVPDQTLCDDVGIRLYLRC
                     QSIVRVRRGQVECPVCQTVFEVPSSSGADPRSCPGAGCSWRITRSEYARSWSKRLLWA
                     GNASEAFEAYFQGYPLARSYSEKILLIDRLIHAFHWDLKADSPNRSAANN"
BASE COUNT         1017 a         1990 c         2005 g         1082 t
ORIGIN      
        1 gcatctcccg aggctggccc aaggtcctct ccagcctgaa gtccctcctg gaggtgggca
       61 agccccttcc gaacctgtgg tagcgtatct cgtcgacacc cacgcgcatc tcagtgactc
      121 gagcttcgac gccgaccgag aggcggtcct gaggcgagca agcgcggcgg gtattgcggc
      181 cgtcctgacc atggcggaga gtccggagga ctgcaaacgc tgtctttcgc tggcaaccga
      241 gtttccgcag gtgctggcgg ctgctgggct ccatcctcag gccgccgagg cgaaggctgc
      301 tgatgatgtg gttgccctca ttcgagacgc ctcggacctt gtggccatag gcgaggtcgg
      361 cctggatttc tggatcgccc aggacgaggc gggacgacag atccagcggg aggtattcga
      421 gcggctcacg cggctttcgg tggagctggg cctcccgctg aacgtgcact cgcgctccgc
      481 gggcaggcaa gccatagagc ttctgatgcg gctcggagcc cgcaaggtcc agatgcacgc
      541 cttcgacggc cgagcgtcca cggcgatgcc ggcggcagaa gccggcttct tcttctccat
      601 ccctccgtcg atcgtccgct ctcggcagaa gcagaagctc gtggaaaggc tgccgctctc
      661 ctgcctgctc ctggaaagcg acagcccggc cctgggaccc gatccgacgg ggcgcaacga
      721 gccggccaac ctgcaggtgg ccgtccgggc catcgcggag atcaagaagg tcccggaagc
      781 cgaggttcgc gccgccacgt gggagaacgc ggtacggctc tatggtgccc ggctcgaagc
      841 ggccgcgatg gcggcccatc agcgttctcc ttgacaccgg gcgagcgaat cctgatattg
      901 atgcaatcgg agaaacgtgt ccttgaaaag tctggcactc gcggcttcct ttctgttcgt
      961 ctccctctct gccctggcgt tcgatttccg ctccgtctcc tggatgatga gccgagacca
     1021 ggtcgttgct gccgagaacc tgtccatcgt ttccgagaac ggattcggcg gccagcagga
     1081 gctttcctac cagacgtccc tgaacgggtt cgccggctcg ctttcctaca ctctggagaa
     1141 tgatcgtctg gtggccgctt ctttcagctt cccgaccgac aatcacgggg agctcttccg
     1201 atatctgaag ggcacgctca tcaggcagta cgggccgccc agctttcaga gcgaccggct
     1261 cataggctgg cggatgaacc gcacggagat cgccctggcc ttcctctccg gccggatctg
     1321 tcacctttcc tactgggaga agtcctactt cgcccggatc aacaacctgt cggtcagcgc
     1381 gtcccgatag ccgactcccg gctccttccc ttccgccaat ccggcccctg gctcatccgc
     1441 gtacctactc cgtgcactcg ggggttggac cccggacgtg ctctctcata ggaggaagag
     1501 acgaatgaga gttagcgctt tacgagtcgc cgtgattgcc gtcgcactga tcgctggcgc
     1561 gggcctggtc tgggggcagt cggcaacgac gatccaggtc aagacgggcc cgaatggcgt
     1621 gggctatctc acggatagca agggaatgac cctgtactac tacaccaagg acgcggacgg
     1681 acagagtgcc tgctacggag gctgcgccaa ggcctggccg gtcttctaca cctcgacggt
     1741 gaccatcccg ccgtccctgg acgcgtcgga tttcggcacg atcacgcgga ccgacggaac
     1801 catgcagacc acctactacg gctggccgct gtactactgg cagaacgaca agagccccgg
     1861 cgacatgacc ggggaaggcg tcggcaaggt ctggtacatc atgaagtccc cctggtattc
     1921 cgtcacggtc agcaccaacg cccagctcgg caactacctc gttgacgaga acggcatggc
     1981 cctctactgg ttcaccaagg attcggtcgg gcagagcgcg tgcatggggg actgcctcaa
     2041 ggcatggccg gcgttcacgg cgacctccat cgtagtgccg tccgcgctca gcgtatccga
     2101 cttcggcacg atcatccggg ccgatgggac catccagatc acctacaagg gctatccgct
     2161 gtactattgg gtgaacgaca agaagcgggg cgacgtcacc ggccagaacg tcggcaaggt
     2221 ctggttcgta atcgaccccg agaagttccc cccgaaaatg tagcttcccc cttctttccg
     2281 catcctgggc cgggtcccgc cgctcgggcc cggccctctt tgccgccacg gcggtcggcg
     2341 cacggcggtg tcatccgcgg ccgaccttga cagacgccgt ctcccgtcgg atcgtgttca
     2401 caacccgatg agcgccaaga agggaagcaa gccccaggga cgagccacgc acgccggttc
     2461 cccgcgatcc cggcatccac cgggtgcgga cgtcttcttc gagactcctg gcctgcgcat
     2521 catccacaac gacctcctca agaccgtctc ggttccagcc cgttccgtgg acctgatcgt
     2581 gacctcgccg ccgtacaacg tggacatcca ctaccagtct catgtcgacc gtctggggta
     2641 tgaggagtac ctcgagttct cccgcacctg gctggcgcgc tgcttgcgat ggctgaaacc
     2701 agatggccgt ctctgtctca acatccctct ggacaagaac aagggcggtc agcagagcgt
     2761 gggggcggac ctcacgagcc tggccaagcg agccggctac cagtaccact cgacgatcgt
     2821 gtggaacgag gggaacatct ctcgccgcac ggcgtggggg tcctggatga gcgcttccgc
     2881 gccctatgtc atcgctcccg tggagctgat cgtggtcctg tacaaggacg tctggaagaa
     2941 gacccgcgcg gaggcccgct cggataccac gcgggacgag ttcatgggat ggacgaacgg
     3001 cctgtggacc ttcaacggcg agaaccgaaa gcgcgcgaac catcccgcgc cctttcccgt
     3061 ggagctgccg cgtcgctgca tcaagctgtt cagctttgtc ggcgacacgg tgctcgatcc
     3121 cttcctgggg agcgggacga cgctcgtggc cgccgcggcg aacggccggt tcgggatcgg
     3181 gatcgagatc gacgaatcct attgcaggct tgcgcgggag cgccttgcca gggagaccgc
     3241 ggtccatcga ggggagacac catgaccgga ctcgcggaat ggctgaaggg gcgccaaccc
     3301 ctgctcctgg acggcgccat gggaacggag ctggaccgcc gtggagtgga agggcgctgc
     3361 ctctgcaatc tatcagggcc tcggcacgtg attgcggtcc accgcgacta ccgggaggcg
     3421 ggaagccacg ccgtcattac caataccctg accatgaatc ggagcttcat cgagagccat
     3481 cggatcgggg tggatgtcct ggaggtgaac cgggccggcg ccgccctggc ccgggaggcg
     3541 gccggacccg gcggcctggt gctggggaac ctgagctcca cggggcagct cctggaaccg
     3601 tacggtcccg tcccggcggc ggtggttctc gaagcgttcc gggagcaggc gcgcgtcctg
     3661 gcggagggag gggtcgacgg gttcatcatc gagaccatga tcgacctgcg ggaggcggag
     3721 tgcgccctga aggcctgtaa agaggtctcc ggcctgccgg taatcgtctg tatcgcgttt
     3781 gacacggcgc aacgcggcgg ccgtaccgtg atgggaaact ccgcggaaca gtgcgttcgg
     3841 tcgctcacgg aagcaggggc ggacgcggtc ggcgccaact gcggaggtgt ggatcccgcg
     3901 cagatggccg agatcgtctc ccggatggtg tccatcagca ccgtgcccgt ggccgcggag
     3961 ccgaacgccg gactgcccaa gctggtcggt gggaagaccg tgttcgacat ggagccgcgg
     4021 agcttcgccc tcgggttggc ggcgtgcgcg aaggccggag caacgatcct gggcggatgc
     4081 tgcgggacca cgcccgacca cattcgggct ttcgccgcgt taggggcaga gccgtggctt
     4141 gtctgatcgt cccgatggcg cagggtcatg ttccgcaggc ggcggagctt ttccgcggcg
     4201 cgtatcagcg tgagcgtcgc tgcagtccgc tcctgcctcc cgagccgctg ggtggcgagg
     4261 ggacgctgag agagcacctg ctggtagacc tggccaaccc cggcgtcgtc gcggtcgacg
     4321 gcggcaggct gatcgggtac ctgtgcgtca gcgcccggtt ccccttcaaa ggcctttccg
     4381 cggccctggt tcgggagcac agtcattcgg ccgccccggg acaagagcca tcacttcttc
     4441 cggagctcta cgccgccctg ggtgagaccc tcatcgagca aggagttcag ctccatatcg
     4501 tcgcgcacct ggcttcggac ggggagctga aggaaacgct ctttcaactc ggcttcggcg
     4561 ctttcctcac ggagcggctg cgtgacctgt cgccggtcga gggcgcggcc ctggcgccca
     4621 tcgagccgct ccgggatccc ggggagctgg tggcgctgga gatggagcat cggcggtact
     4681 acctgcagtc cccgatcttc ctcaccaagg acgacacccg agaggctgcg ctggcagggc
     4741 tctgcgagga ccgagacagc ggcaggacgg cgctggtcta ccgggaccat gggacgcccg
     4801 cggcctatat ggtcgtgggt ccctgttccg cggagcagga ggggcgcctg ctgcggggca
     4861 gcaagacggc ccaggtgtgc tccgcctacg cgacgccgtc ggtccgcggc agggggatcg
     4921 gccgcgcgct cctccagggt tgcgtggact gggcgcggtg cgccggctac gaacggctgt
     4981 tcgtcgagca cgagacggcg aacctggcgg gtcgccggtt ctggggacgg cacttcgcgc
     5041 cgtatctgca cttctccatg cggtacgttg agggcaggta gggggcacac catggatttc
     5101 tcaagggtca cgacgccgca gagggtttcc ctcctcatct cgttcctgga tatccagggg
     5161 ttcctgggca tctcccagaa gctgaacgat cccttggatc tgtttgccct gctcgacgat
     5221 ctggcgaaga tgatcatcca tgacgtggat gccagctcgg ggagggtcat caagctcatc
     5281 ggggactccg ccctcgtggt ctaccccgag gaggcgatcg accgcggggt caacgcgctc
     5341 ctggccttga agacccggct ggaagaggac ctttcccgac ggggattctc aaatcggttg
     5401 cgcatgaccg cgcacttcgg ggaggcggcc atcgggctcc tgggcgaggg gcagtgccgg
     5461 cacctcgacg tcctgggaga ctccgtgaac accgcggcca tgctggagcg caccggtcac
     5521 ggcggcaggc tcatcctctc tccgcaggcg tttcgacgat tgtcctcctc cacgcggaag
     5581 ctcttccacc ggtttacccc gccccaggtc tacctggccg aggagcgctg aaggggcatg
     5641 accgagcgga tccggtggtc cccgcggatt caccctgacc tggtgcggcg tctctatgag
     5701 gccgacggcc ggggcgtgcc ggaccagact ctctgtgacg acgttgggat ccggctctac
     5761 ctccgctgcc agagcatcgt gagagtgcgc cgcggccagg tggagtgccc ggtgtgccaa
     5821 acggtgttcg aggttccgtc ctcatctggt gccgacccac gatcgtgccc cggggcgggt
     5881 tgctcctggc ggatcacccg atcggaatat gcacgaagct ggagcaaacg tctgctctgg
     5941 gccggcaacg cttctgaagc atttgaggcc tactttcaag gctatcctct ggctcggtcc
     6001 tactcggaga agatcctcct catcgaccgt ctgatccacg cctttcactg ggacctgaag
     6061 gctgactccc ccaaccggtc cgccgccaac aacc
//
LOCUS       DASRZS010000036         3460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5022047, whole genome shotgun sequence.
ACCESSION   DASRZS010000036 DASRZS010000000
VERSION     DASRZS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5022047"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..2512)
                     /gene="lon"
                     /locus_tag="VFH83_00950"
     CDS             complement(38..2512)
                     /gene="lon"
                     /locus_tag="VFH83_00950"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET6484952.1"
                     /translation="MAGFLRANRGGGSGGVPDELAILPLRDTVAFPSAVMPLAVGIAR
                     SVKLVEEAAQKDSLIGLVTSRDATVEEPGPQQVHQIGTAGRILRMARTTDGSLQVVVQ
                     GTHRFRVRHWLTPAAYLRARVEPAPEVTESDLETEGLVQGLRDLAHQVIELSPNIPKE
                     ASGFLSSVGDALSLAYLVASNAQLPTAEALAVLEADAVKEKLRILIRHLEHQREVLTI
                     GQKIRSEAKETMEKEQRDYILRQQMKAIQKELGDLDENQSIVAEYTAKLEKAGMPEEA
                     LKEARRELSRIGGMPAQAAEYSMIKTYLDWMVELPWSTVSDDQLDITHARKVLDEDHY
                     DLQEVKDRIVEFLAVRKLLRERGILTLAADEGKAGRAMGAILCFVGPPGVGKTSLGQS
                     IARALGRKFTRMSLGGMRDEAEIRGHRRTYIGSMPGRVMQAVKRAGTRNPVFMLDEID
                     KVGSDWRGDPSSALLEVLDPAQNGTFRDHYLNVDFDLSEVMFITTANQLEPIPAPLRD
                     RMEIIHLDGYTEHEKVHIAQEHLVPRQLAANGLRETEVVFSEESLRKLVADYTREAGV
                     RNLERQIGAICRKSVARIAGGEVSTVEVTPELVRELLRKERFESESSESIEIPGIATG
                     LAVTPVGGDILFIEATRMKGKGEFTLTGQLGDVMRESARIAYSYVRSKATELGVNPEV
                     FSQFDIHVHVPAGAIPKDGPSAGVAMAMAIASLFMARPVRGDVGMTGEVTLRGRVLPI
                     GGVKMKVLAAHRAGLTTVILPKRNERDLEELPQDVREAMRFVAVDRIDEAFAVAIGAA
                     DGLAVGVAAQGDRLIASAGAVPAAAQ"
     gene            2835..3209
                     /locus_tag="VFH83_00955"
     CDS             2835..3209
                     /locus_tag="VFH83_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484953.1"
                     /translation="MRTRLLGIVGVAAVLLTGAVFSASAMPAGNAVAPSDAILQFFAS
                     HGIDTQYIGMSQDKVPADVWAKIVALQSDPDLVTLVQANLRDSFNFDGHLLYEQVGDV
                     YYEAEASMNQKGAPSFNWEGIG"
     gene            3275..>3460
                     /locus_tag="VFH83_00960"
     CDS             3275..>3460
                     /locus_tag="VFH83_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HET6484954.1"
                     /translation="MQNTVTAERRNSRGEENILSLYLKEINRIPLLTREEEERLARRA
                     AKGEQLAKESLIRANLRF"
BASE COUNT          581 a         1145 c         1091 g          643 t
ORIGIN      
        1 ccaccttccg gccggccccg cgcttccaga cgagtcgcta ttgggcggcg gcaggaacgg
       61 cgccggcgga ggcgatgagg cgatccccct gagccgcgac ccccacggcc agcccgtccg
      121 ccgctccgat tgccacggcg aacgcttcgt cgatccggtc caccgccacg aaccgcatgg
      181 cctcgcgaac gtcctgaggc agttcttcca gatcccgctc gttgcgcttg ggcaggatca
      241 ccgtagtcag ccccgcgcga tgggcggcca ggaccttcat cttgacgccg ccgatgggga
      301 ggacgcgtcc ccgcagcgtc acctcgccgg tcatgcccac gtctccgcga accggccgag
      361 ccatgaaaag gcttgcgatc gccatggcca tggcaacgcc cgcggacggg ccatccttgg
      421 gaatggcgcc cgccgggacg tgcacgtgga tgtcgaactg ggagaagacc tcagggttca
      481 ccccgagctc ggttgccttg gacctgacgt agctgtaggc gatccgcgcg ctttcccgca
      541 tcacgtcgcc gagctgcccc gtgagcgtga actcgccctt gcccttcatc cgggttgcct
      601 cgatgaagag gatgtctccc ccgacgggag tcaccgccaa ccccgtggct atcccgggaa
      661 tctctatgga ctcggaggac tcggactcga agcgctcctt gcgaaggagc tcccgaacga
      721 gctccggggt cacttcgacc gtcgacacct cccccccggc gatgcgggcc acgctcttgc
      781 ggcagatggc gccgatctgc cgttccaggt tgcggacccc ggcctcccgg gtgtagtccg
      841 caacgagctt gcgcagcgac tcctcgctga agaccacctc cgtctcccga aggccgttag
      901 ccgccagttg cctcgggacc aggtgctcct gggcgatgtg gaccttctcg tgctcggtat
      961 agccgtccag gtggatgatc tccatgcggt cccgcagggg cgcggggatg ggttccagct
     1021 ggttggccgt ggtgatgaac atcacctcgc tcaggtcgaa gtccacgttg aggtagtgat
     1081 cgcggaaggt gccgttctgg gccggatcca ggacttccag cagggcgctg gatggatccc
     1141 cgcgccagtc gcttccgacc ttgtcgatct cgtcgagcat gaagaccggg ttgcgcgtgc
     1201 cagcccgttt gacggcctgc atgacgcgcc ccggcatgga cccgatgtag gtgcgccggt
     1261 gccctcggat ctccgcttcg tctctcatgc cgccgaggct catccgggtg aactttcgac
     1321 ccagggcccg ggcgatgctc tggccgaggg aggtcttccc gacaccggga ggacccacga
     1381 agcagaggat tgcccccatg gccctgccgg ccttgccctc gtccgcggca agcgtgagga
     1441 tccctcgctc ccggaggagc ttgcggaccg cgaggaactc cacgatccgg tccttgacct
     1501 cctgcaggtc gtagtggtcc tcgtccagga ccttgcgggc gtgcgtgatg tcgagctggt
     1561 cgtcgctcac ggtgctccac gggagctcca ccatccagtc gagatacgtc ttgatcatcg
     1621 agtactcggc tgcctgggcc ggcatcccgc cgattcggga gagctcgcgg cgcgcttcct
     1681 tgagcgcctc ctcgggcatt cccgcctttt ccagcttcgc ggtgtactcg gcaacgatgg
     1741 actggttctc atccagatcc cccagctcct tctggatggc cttcatctgc tgacgcagga
     1801 tgtaatcccg ctgctccttc tccatggtct ccttggcctc ggagcggatc ttctggccga
     1861 tggtgagcac ctcccgctgg tgctcgagat gtcgaatgag gatgcggagc ttctccttca
     1921 cggcatcggc ctcgagcacc gcaagagcct ccgccgtggg aagctgcgcg ttggaggcta
     1981 ccaggtatgc gagggagagg gcgtctccga cggatgagag gaatccggag gcctccttgg
     2041 ggatgttcgg tgagagctcg atgacctggt gggccaggtc acgcagaccc tggaccaggc
     2101 cctcggtctc caggtcggat tccgtgacct cgggggccgg ctccacgcgc gcccgaagat
     2161 acgcggccgg ggtcagccag tggcgcaccc gaaaccggtg agtcccctgg accacgacct
     2221 gcaggcttcc atccgtggtg cgcgccatgc gcaggatccg gcctgcggtg ccgatctggt
     2281 gaacctgctg agggcccggc tcttctaccg tggcatcccg ggaggtcacc aggccgatga
     2341 ggctgtcctt ctgggccgcc tcctccacca gcttgaccga ccgcgcgatc cccacggcaa
     2401 ggggcatgac cgcggaagga aatgcaaccg tgtctcgcag cggtagaatc gccaattcat
     2461 caggaacgcc accggatcca ccgccgcggt tcgctctcag gaatcctgcc attctagtta
     2521 cctcgtcttc tttccggggc aaagatacta gagcatccgg gtgaagccaa ataacgggct
     2581 gttctatgga aaaggatcca ggacccgaat gcggtgctct ggatcgccgc cggcgcgcgg
     2641 gcccctcgcc gggttataaa acgccccata ccgaagccgt tcatgttcgt ctatcgaaaa
     2701 ttcatccccc gtctatggtt cccaaagcct gggagatcga tcctctcttt gtcgaatgcg
     2761 ggtgttgccc gccagtaacg ctcgtcgggg cgcccaggat ctgggcattc ccgacgacga
     2821 ccaaggagtt ccttatgagg acgagactac tcggcatcgt cggtgtcgcg gcggtcctgt
     2881 tgacgggggc ggtattcagc gcatcagcga tgcctgcggg gaatgcagtt gctccaagcg
     2941 acgcaatcct gcagttcttc gcttcgcacg ggatcgacac ccagtacatc gggatgagcc
     3001 aggacaaggt cccggcggat gtgtgggcca agatcgttgc gcttcagtcg gatcccgacc
     3061 ttgtgacgct ggtccaggcc aatcttcggg attcgttcaa ctttgacggg cacctgctct
     3121 acgagcaggt gggggatgtc tactacgagg ccgaggcttc catgaaccag aagggggcgc
     3181 cctccttcaa ctgggaaggc attggctgat ccccgtgcgc tctgaaaaga ccctgccgaa
     3241 cgcgcagggt cttttcgttg agaggagaaa cggaatgcag aacaccgtaa ccgcagaacg
     3301 tcgtaactct cgcggagagg agaacatcct ctccctctac ctgaaggaga tcaaccggat
     3361 ccccttgctc acgcgggaag aggaggagcg actggctcgg agggcggcca aaggcgaaca
     3421 gctcgccaag gagagcctga tccgcgccaa cctgcgcttc
//
LOCUS       DASRZS010000037         4537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5023946, whole genome shotgun sequence.
ACCESSION   DASRZS010000037 DASRZS010000000
VERSION     DASRZS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5023946"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VFH83_00965"
     CDS             <1..431
                     /locus_tag="VFH83_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET6484955.1"
                     /translation="VGNLLAGKTYDFELIRGGTRMTVPVKLAVRPADGSDSLAYRNLW
                     PGMTVVRINDQIRNDPNLSIAKGLDGVVIAVVMDGGNGRDNQSPAAIAGLQTGDVITQ
                     INGKDVRTVADFYKDLADKTRTVTFKINRAGTELTIGITR"
     gene            complement(513..2987)
                     /locus_tag="VFH83_00970"
     CDS             complement(513..2987)
                     /locus_tag="VFH83_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET6484956.1"
                     /translation="MKKWFLWIATGALLAVLLACQTTPTAPQPSTIQPKSGGLAPSGQ
                     GHTTLDLALTFGNADLIQTWKVEIVGTAGTVRTWTGDAKNAPAAVTWDGKNDSGAVAS
                     EGTYTARLSVAYQKTYQPATAQSSSFLLDVTPPTGSIQFDPTQLVPTSQGVQAPTTIT
                     ITASSAVAKMDSWSLAIQDASGNAFRTFTGTWSNTSVPWDGTSTTGAAVTPSTTYTAV
                     ATVQDEFGNSAQIKGSIAVAQAPAAPAAPAAPAQAQTAPPSQIQPRLSGFSPLSDTLP
                     QSIGLLVSVGAPDALKSWRLSVGQNGQPPQRTWTGDASNVPGTVTWDGKTDGGAQAPE
                     GTYTATLSVDYGDAYLAAQAQSKSFVLDVSPPTGRVTANPANLTPNGQGGIQPESFSL
                     TATSKTASVLSWNLSVLGPDNTPVALFNGLDPAAKVSWDGTESGGGTVDPTLVYSLKA
                     QVRDVYGNVGVIQTPLPGGALPPVTGTVSATAQLTGFSPNSDTLPKAQTIALSLGQPS
                     AVTSWSVGISQAGKGAVRTYEGDGSNVPASISWDGKADDGTMAPDGMYTADLTVSYGK
                     TYRAASASSAPFVLDVTPPMGSIALSTPLFSPVEGPSTITLTVSASSPVAKIDSWSMT
                     IYDPTGSAFNSFSGKWPNSQVTWDGKDAKGDLVQSAMDYDAVASIRDEFGNVGQTKVT
                     VPVDILVEKTATGFRILDSRIFFQEFTADYMAVSPDLAKQNVARLDALTEKLKKFPGY
                     RIQIVGHAVSIYWWDPQQAKLEQQDILIPLSKARAEAVKKALVDRGIDPAVLSTDGVG
                     AADQLVPDSDLVNRWQNRRVALYIER"
     gene            complement(3210..3839)
                     /locus_tag="VFH83_00975"
     CDS             complement(3210..3839)
                     /locus_tag="VFH83_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6484957.1"
                     /translation="MIQTIVCDDHPIFREGLKKIVGQSTDIRVAAEASEGRELLEQLE
                     RRRFDMIILDITLPGSSGLELLRELKRVAAPPVLILSMHPEEQYASRAIAAGAGGYLS
                     KSSVPEELLTAIRQVAQGKRYVSRTLAEHLADQLAEGDLRQPAHTRLSQREFEVLRLL
                     AAGRKTKEIAEELYVSSPTVATHRARILEKLHMTSTAELVRYAVENHLV"
     gene            complement(3836..>4537)
                     /locus_tag="VFH83_00980"
     CDS             complement(3836..>4537)
                     /locus_tag="VFH83_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6484958.1"
                     /translation="TRGKKARMALERSREELRSLASHLQALREEERTRVAREVHDELG
                     GLLTTLKMELSLVARRGSRNSCQEEYHSALELIEQSLAMIHRIAADLRPVVLDDFGLF
                     PALEWYLRDFQKRTGISCDLRADPVEWTLDRSSAASLFRVVQEALTNVARHAHATTVT
                     VDLHREGGRLRVSVEDNGVGIDEARLANTRSYGLIGIRERVLSLGGELAIRGAPGRGT
                     TVSLSIPEGVLGGTP"
BASE COUNT          720 a         1515 c         1503 g          799 t
ORIGIN      
        1 tggtgggaaa cctccttgcc ggcaagacct acgacttcga gctcatccgt ggcggcaccc
       61 gcatgacggt acccgtcaag ctggcggttc gcccggcgga cgggagtgac tccctggcct
      121 accgcaacct gtggcccggc atgacggtgg tgcgcatcaa cgaccagatc cgcaacgacc
      181 cgaacctcag catcgccaag ggcctggacg gcgtggtgat cgcggtcgtg atggacggcg
      241 ggaacggaag ggataaccag tccccggccg ccattgcggg gctccagacg ggagacgtga
      301 tcacgcagat caacggcaag gacgtccgga cggtcgctga cttctacaag gacctggcgg
      361 acaagacccg caccgtgacc ttcaagatca atcgcgcggg caccgagctg accatcggga
      421 ttacgcggta gcgcggccga gatcacgcgg taccaacgag ggccgcgtct ccctggggag
      481 cgcggccctt tctttgcacc tgcgttctgt cgttatcgtt cgatatacaa cgccacgcgt
      541 cggttctgcc agcggttgac caggtcgctg tcgggcacca gctgatccgc ggctccgacc
      601 ccgtccgtgc tcaggaccgc cggatcgatc ccccgatcca ccagcgcctt cttgacggcc
      661 tccgctcggg ccttggagag cgggatgagg atatcctgct gctccagctt ggcctgctgc
      721 gggtcccacc agtagatcga gacggcgtgg cccacgatct ggatcctgta ccccgggaac
      781 ttcttcagct tctcggtcag ggcgtccagg cgcgcgacgt tctgcttcgc cagatccggg
      841 ctcacggcca tgtagtccgc cgtgaactcc tggaagaaga tgcgggagtc gaggatgcgg
      901 aagccggtcg cggtcttttc cacgaggatg tccacgggaa cggtgacctt ggtctgaccg
      961 acgttgccga actcatcgcg gatgctcgcc acggcgtcgt agtccatggc ggattggacc
     1021 aagtctccct tggcgtcctt cccatcccag gtcacctggc tgttcggcca cttgccgctg
     1081 aaggagttga atgccgaccc cgttggatcg tagatggtca tcgaccagct atcgatcttc
     1141 gcgaccgggg agctggcgct cacggtcagc gtgatggtgc tcggcccctc gacaggagag
     1201 aagagcggag tcgagagggc gatggatccc atcggcgggg tgacgtccag gacgaacggc
     1261 gcgctggacg cggaggccgc gcgatacgtc ttgccgtagc tcaccgtcag atcggcggtg
     1321 tacatgccgt ccggcgccat cgtcccgtca tcggccttcc cgtcccaact tatcgaggcc
     1381 ggcacgttgc tcccgtcacc ttcgtaggtt cggaccgctc ccttgcccgc ctggcttatc
     1441 ccgacggacc acgatgttac ggccgaaggc tgacccaagg acagcgcgat ggtctgggcc
     1501 ttgggcaggg tgtcgctgtt gggggagaac ccggtgagct gcgccgtggc ggacacggta
     1561 cccgtcaccg gcggcagcgc cccgcccgga agcggagtct ggatcactcc gacgttcccg
     1621 tacacgtcac ggacctgggc cttgagggag tacaccagcg tggggtcgac ggtgccgccg
     1681 ccggactccg tcccgtccca ggaaaccttg gctgcggggt ccaggccatt gaacagcgcc
     1741 acgggcgtgt tgtccggccc caggacgctc agattccagc tcaggacgga agccgtcttg
     1801 gaggtggcgg tcaggctaaa gctctccggc tggatccctc cctgaccgtt cggggtgagg
     1861 ttggccgggt ttgcagtcac gcgaccggtc ggcgggctga cgtcgagcac gaaggacttg
     1921 ctctgcgctt gggccgccag gtaggcgtcc ccgtagtcca cggagagtgt ggcagtgtag
     1981 gtcccctctg gcgcctgggc gccaccgtcc gtctttccgt cccaggttac ggtgcccggg
     2041 acgttcgagg cgtccccggt ccaggtcctc tggggcggct ggccgttctg ccctaccgac
     2101 agcctccagg acttgagcgc gtcaggggcc ccgacagaca ccagcaaacc gatgctctgc
     2161 ggcagcgtat cactgagcgg agagaatccc gagagccgag gctggatctg ggaaggcgga
     2221 gcggtctggg cttgcgccgg cgcggccggc gcggctgggg ctgccggggc ctgtgccacc
     2281 gctatggacc ccttgatctg cgcgctgttc ccgaactcgt cctgaaccgt tgccaccgcg
     2341 gtataggtgg tgctcggcgt aacggcggct ccggtggtcg aggtgccgtc ccacgggacc
     2401 gaggtgttcg accaggtgcc ggtgaaggtc cggaaggcgt tgccggaagc gtcctgtatg
     2461 gcgagggacc agctatccat cttggccacc gcggagctcg cggtaatcgt aatcgtggta
     2521 ggcgcctgca cgccctggct ggtgggaacg agctgggtgg gatcgaactg aatggacccc
     2581 gtgggcggag tgacatccag caggaagctc gagctttgcg ccgttgccgg ctggtaggtc
     2641 ttctggtagg cgaccgacag gcgcgccgtg tacgtgcctt cggaggccac cgcgccgctg
     2701 tcgttcttgc cgtcccaggt caccgccgcc ggagcgttct tggcgtcccc ggtccatgtc
     2761 cgtaccgtgc ccgcggtccc gacgatctcc accttccacg tctggatcag atccgcgttt
     2821 ccgaacgtca gggcgaggtc cagagttgtg tggccctgcc cgctcggcgc aagaccgccg
     2881 gacttcggct ggatcgtgct gggctgcggg gccgttggcg tggtctggca cgcgagcagc
     2941 accgccagga gagcgcccgt tgcgatccaa aggaaccatt tcttcatgcc tacctcctcc
     3001 gggagacgga ctcctcctct cccgttgatc gatgcaaggg cggtacccgt tccgcccaca
     3061 ggaccaaagc taaaaagccc gaacgccggc ctccaaccgg cccattgatg gaagcttacc
     3121 ctctaccgcc gcgcctcctg tcgcacgaac gatgatttta agatcgaatc tgttcgacag
     3181 aaagggggca aacccacgcg ggggccggcc tacaccaggt gattctccac tgcatagcga
     3241 accagctccg cggtagaagt catgtggagc ttctcgagaa tccgggcacg gtgggtggcg
     3301 accgtcgggg agctcacgta cagctcctcc gcgatctcct tggtcttgcg cccggcggcc
     3361 aggagcctca agacctcaaa ctcccgctgg gacagccggg tgtgggccgg ctggcgcagg
     3421 tccccttccg caagctggtc cgccaggtgc tccgccagcg tccggctcac gtaccgcttg
     3481 ccctgggcca cctgtcggat cgccgtcagc agctcctccg ggacgctgga cttgctgagg
     3541 tatccgccgg cgccggcggc gatggcccgg gaagcgtact gctcctccgg atgcatgctg
     3601 agaatcagaa ccgggggagc cgcgacgcgc ttgagctcgc ggagcagctc cagcccgctc
     3661 gatcccggca acgtgatgtc caggatgatc atgtcgaatc gacgccgctc gagctgttcc
     3721 aggagctccc gtccctcaga cgcttcggcc gccacccgga tgtccgtgga ttggcccacg
     3781 attttcttga gcccctcccg gaagatggga tggtcgtcac acacgatggt ctggatcatg
     3841 gggtccctcc caagacgccc tccggtatgc tgaggctgac cgtggttccc cgacccgggg
     3901 cgccccggat ggccagctcg ccccccaagg agagcacgcg ctcgcggatc ccgatcagcc
     3961 cgtacgagcg ggtgttcgcc aggcgcgcct cgtcgatgcc aactccgttg tcctccacgc
     4021 tgacccgaag ccgaccgccc tcgcggtgaa ggtccacggt gacggtcgtc gcgtgggcgt
     4081 ggcgggccac gttggtgagc gcttcctgca cgacacggaa cagcgaggcc gccgaggatc
     4141 ggtccagggt ccattccacc ggatcggcgc gcaggtcaca agagatcccg gtgcgcttct
     4201 ggaagtcccg aaggtaccac tccagggccg gaaagagccc gaagtcgtcc aacacgaccg
     4261 gtcggaggtc cgccgcgatg cgatggatca tggccagcga ctgctcgatg agctccagcg
     4321 ccgaatggta ttcctcctgg caggagttgc gcgatccgcg gcgcgccacc agggagagct
     4381 ccatcttgag ggtcgtcagc agcccgccca gctcgtcgtg cacctcgcgc gccacgcggg
     4441 tccgctcctc ttctcggagc gcctgcaggt gggaagcgag gctcctcagc tcctcccggg
     4501 acctttccag cgccatgcgc gcctttttcc cgcgggt
//
LOCUS       DASRZS010000038         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3869707, whole genome shotgun sequence.
ACCESSION   DASRZS010000038 DASRZS010000000
VERSION     DASRZS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3869707"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VFH83_00985"
     CDS             complement(<1..1024)
                     /locus_tag="VFH83_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484959.1"
                     /translation="MRFPAVPRVAPLAAAALILSGAVVLGVLQVRWVEAATAAEVSRQ
                     RQEIGRGAFQVAAASADEERVLVSVSRISAADFAAGTWDSISSTLGLWYRYTRFPNLL
                     NGAYILRSPRSRGGLAYARSQARFVDAVVPRDVVQAAGAALSAGQPQRGWETVTPGDG
                     DGRRIVVLPVGGPTGDLGAVVLDLAEAVIYRHVVQYFMTQYLPDYPYRLVTADGTAIF
                     STASVKQGDPPEAVVGLEGLNGPGPGLSIGPQSSAAGTGASYVDPLLQSWLQRAQGNA
                     PAFSEPPGRGERADVLLQVFYPNQSLSRAMRRQAAVNILVSLGILAFLVASMAVLSGL
                     YRRSARL"
     gene            1141..>2371
                     /locus_tag="VFH83_00990"
     CDS             1141..>2371
                     /locus_tag="VFH83_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6484960.1"
                     /translation="MSGHLAAITENLFRFEDTCNVYAIRQGNQALLVDFGSGHILEHL
                     GEIGVEQVVAILHTHHHRDQAQGDPLAVARGIPIHVPQHERQLFDQVETFWTTKQLYD
                     VYNVRNTYFTLTRSVPVQGVLADWERFSWKGISLEILPTAGHTVGSISLVGRVDGDLV
                     AFVGDLLYSAGKVQSLYDLQYAYGAMDGVETAILSLNLLEDRGPRMLCPSHGEPMRDV
                     GAAFRQTRDNLRSLFRLQSGGQLAADEIDFTPVAGRLLAAVQACSSFYVILSRDGRRA
                     LLVDYGAPNFSLFQPASHRFEGGDRVRFVHHSLDRLSTQYGVKTIEAVLPSHYHDDHI
                     NGIPYLQRAMGTQVWAYENMKEILEKPGGELIGCVLPDPIQVSRTFTDGERFSWEGLD
                     FEIHYTPGHADYHMALFT"
BASE COUNT          405 a          818 c          785 g          363 t
ORIGIN      
        1 gcagtcgagc gctcctgcga tagagaccag agaggacggc catgcttgcg acgaggaacg
       61 cgagaatccc gagactcacc aggatgttca cggccgcctg gcggcgcatg gcgcgagaga
      121 ggctctggtt cgggtagaag acctggagca gcacgtccgc ccgttcgccg cggcccggag
      181 gctccgagaa cgcaggggcg ttcccctggg cccgctggag ccaggactgc aggagcggat
      241 ccacgtagga tgcccccgtg cctgccgcgg aggattgcgg tccgatcgag agtcccgggc
      301 caggcccgtt caacccttcc agtcccacca ccgcttccgg agggtcgccc tgtttcacgg
      361 aagcggtcga gaagatggcc gtgccatccg cggtcaccag gcggtacggg tagtccggaa
      421 gatactgcgt catgaagtac tggacgacgt ggcggtagat cactgcctcg gcaaggtcca
      481 ggaccaccgc tccgagatcc cccgtgggcc cgccgaccgg aaggacgacg atccgtctcc
      541 cgtctccatc tccaggggtg accgtttccc acccgcgctg aggctgcccg gcggagagcg
      601 cggcgccggc cgcctggacc acgtcccgtg ggacgacggc atccacgaac ctggcctgag
      661 accgcgcgta cgccagtccg cctcgagacc gcggggaccg aaggatgtag gcgccgttca
      721 ggagattcgg gaagcgcgtg tagcggtacc agaggccgag ggtggacgag atcgagtccc
      781 acgtccccgc ggcgaagtcg gccgcgctga tccgcgacac ggacaccagg actcgctcct
      841 catccgcgga ggccgcggca acctggaagg cgccgcgccc gatctcctgc cgttgacggc
      901 tcacctccgc ggcggttgcg gcctcgaccc agcgcacctg gagcacgccg aggacgaccg
      961 cgccggagag gatcagcgcg gcagcggcaa gcggagcgac ccgtggaacg gcggggaacc
     1021 gcatgcctct cagtgtacgc cgggcaccgt ggctcgcaag cccccaccgt gcgcggttgt
     1081 tggcgcgtgt ggctcgcaag cccccaccgg ggaggttgca gcccgtggta cactggcccc
     1141 atgagcggac acctcgctgc gatcacggag aacctcttcc ggttcgagga cacgtgcaac
     1201 gtctacgcca tccgacaggg aaaccaggcg ctcctcgtcg atttcggctc cggccacatc
     1261 ctggagcacc tcggggagat cggcgtggag caggtagtcg ccatcctcca cacccaccac
     1321 catcgggacc aggcccaggg ggatccgctg gccgttgccc gcgggatccc catccacgtg
     1381 ccccagcacg aacggcagct cttcgaccag gtggagacct tctggaccac caaacagctc
     1441 tacgacgtgt acaacgtccg caacacctac ttcacgctga cccgcagcgt gccggtccag
     1501 ggcgttctgg ccgactggga gcgtttctcg tggaagggga tcagtctgga gatcctgccc
     1561 acggccgggc acacggtcgg atcgatatcg ctggtggggc gcgtggacgg ggacctggtg
     1621 gcattcgtcg gagacctcct gtactccgcc ggaaaggtcc agagcctcta cgacctgcag
     1681 tacgcctacg gcgcgatgga cggcgttgag accgccatcc tctcgctgaa tctgctggag
     1741 gatcgcggac cccggatgct ctgcccttcc cacggcgagc cgatgcggga cgttggggcc
     1801 gcgttccgac agacccgaga caacctccgc agcctgttcc ggctgcagag cggaggccag
     1861 ctcgccgctg acgagatcga cttcaccccg gttgccggcc ggctcctggc cgccgtgcag
     1921 gcctgctcct ccttctacgt catcctctcc cgggacggcc ggcgcgcgct cctggtggac
     1981 tacggggcgc cgaacttctc cctgttccag ccggccagcc atcggttcga gggtggtgac
     2041 cgggtccggt tcgtccacca ctccctggat cggctttcta cccagtacgg ggtgaagacc
     2101 atcgaggcgg tgctccccag ccactatcac gacgaccaca tcaacgggat cccctacctg
     2161 cagcgggcca tggggacgca ggtctgggct tacgagaaca tgaaggaaat tctggagaag
     2221 ccgggagggg agctcattgg ctgcgtgctg ccggatccca tccaggtttc ccgcacgttc
     2281 acggacggcg agcggttctc ctgggagggt ctggatttcg agatccacta cacgccgggc
     2341 cacgcggatt accacatggc cctgttcacg a
//
LOCUS       DASRZS010000039         4039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774 k141_23715,
            whole genome shotgun sequence.
ACCESSION   DASRZS010000039 DASRZS010000000
VERSION     DASRZS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23715"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VFH83_00995"
     CDS             complement(<1..980)
                     /locus_tag="VFH83_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484961.1"
                     /translation="MNDRERFLSILAFHPVDRVPNYELGLWGQTIDRWYDEGLPRDAC
                     YHNQFEGEPFFKIDRRAFAHVTVSMVPPFEYEELEEDERYLVARHTNGIVTRALKEGT
                     VRGTRASMDQYIGFPVTDRKSFQDLKKRYDPDSPCRYPQWWDMMVDFWKKRDYPLCLL
                     GNGTAGMYSQLRSWVGTENLSYLFFDDPALAEEMVEFNVEFVLKTTARARREIQFDYF
                     NFFEDFAGKGGPLVSPALFRKFLMPGYKRIIEEFRRSGITQFWLDSDGDPRALIPLML
                     EAGITCLWPLEIASDMDPLALRKCYGTSLALAGGIDKRELAKGRKEIEREL"
     gene            1320..3572
                     /locus_tag="VFH83_01000"
     CDS             1320..3572
                     /locus_tag="VFH83_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6484962.1"
                     /translation="MQKLAVCASGFLLFAVLLGCQTTPTQAPRSAILLQSGGAERDGT
                     QGPSVVELGLSFGGADLVKSWKVDIANAEGSVRSWTGDGKRLPTTLSWDGKSDAGAPA
                     PEGSYVARLSIDYQKTYEAAFAESAGFALDITPQGDMAPFDASRLAPGVYPSWPASSA
                     AKTAGFSPRSDTLARTEKIALSFGQASAVTDWKLTIAQPGQAAVRTFQGDGTRLPAAV
                     IWDGRSDDGTAAPDGTYTAQLSVVYGSASQPVQVTSKPFVLDVTPPSGAVVADPPALT
                     ANEGGEIQAESFSLSASSKVASIQSWTLSVIGPDNKPVASFSGLEPDAHVPWDGKLAN
                     GASVDPAQTYSVLAQVQDAYGNVGVIQGSLNRIAVASVESAPPVRDPAGGKQPASPKQ
                     PEIASPAATEEPSAIPELAGFSPQSDTLARMQTIALSYGQASLVTAWNLAIAQSGRAA
                     ARSFQGDGTKLPASVVWDGLCDDGTPAPEGTYTAQLSVSYRDASRPALATSQPFVLDV
                     TPPKGSISLSAPLFSPREGADTLTLSLAARSSTARIDSWSMDIYDPDGNLFNSLHGQW
                     PTSQAVWDGKGLGGARIESAESYPVVARIRDEFGNVGEAKIIVPVDILVEKTAAGNCI
                     LTSNIYFKDFTADCQHVAPDLAKQNVARLDALAKKLKKFPDYKIRIVGHAVAIYWFNP
                     ALAKREQRDVLIPLSKARAEAIKRALVARGINGAKLSTEGVGASDQSVSDRNLADRWQ
                     NRQVDLYVEK"
     gene            3649..>4039
                     /locus_tag="VFH83_01005"
     CDS             3649..>4039
                     /locus_tag="VFH83_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484963.1"
                     /translation="MKSRGFIALLVALAGVALLSGCKPDPVATGMASLDCSSCHTGSA
                     TSTYGLQMLANEGGYANSGHYNGIRELNTAAAVTSSLELYEFSGSHAMNENSANCSQC
                     HTAQGFLTWLSTGTAGVQASSSPPGCFT"
BASE COUNT          757 a         1294 c         1319 g          669 t
ORIGIN      
        1 agctcgcgct cgatctcctt tcggcctttg gcgagctcgc gcttgtcgat cccgccggcc
       61 agcgctaggg aggtaccgta gcatttgcgc agcgcgaggg ggtccatatc cgacgcgatc
      121 tccaggggcc acaggcaggt gatccccgcc tcgagcatga gagggatgag cgctcgggga
      181 tccccatcgc tgtccagcca gaattgggtg atgcccgatc tgcgaaactc ctcgatgatg
      241 cgcttgtacc cgggcatgag gaacttccga aacagcgcgg gagagacgag cgggccgccc
      301 ttgccggcga aatcctcgaa gaagttgaaa tagtcgaact ggatctcccg ccgcgcgcgg
      361 gccgtggtct tgaggacgaa ctccacgttg aactcgacca tctcctccgc cagcgcgggg
      421 tcgtcgaaga aaaggtagga caggttctcg gttcccaccc agcttcgaag ctgggagtac
      481 atgcccgccg tcccgttgcc caggaggcat agcgggtagt cgcgcttctt ccagaagtcg
      541 accatcatgt cccaccactg gggatagcgg catggcgaat ccgggtcgta gcgcttcttg
      601 aggtcctgaa agctcttgcg gtccgtgacc ggaaagccga tgtactggtc catggaagcg
      661 cgggtgcccc ggacggtccc ctccttcaaa gccctcgtga cgatcccgtt ggtgtgacgc
      721 gccacgaggt agcgctcgtc ctcttccagc tcctcgtact cgaacggggg aaccatgctc
      781 acggtgacat gggcgaacgc gcgtcgatcg atcttgaaga agggctctcc ctcgaactgg
      841 ttgtgatagc aggcgtcgcg cggcaggcct tcgtcgtacc aacggtcaat ggtctgaccc
      901 cagaggccca gctcgtagtt gggtactcgg tccaccgggt ggaacgcaag gatgctcagg
      961 aaacgttcgc ggtcattcat ggcggtcgac tcctgttgtg gagggggcca aggcgcgccc
     1021 ggaacctgga tggcccaacc taccatggat cgaccgaggc gagtaagggg tggcgtggag
     1081 tcggcgatct cccgttttct gcactgaaat ccaccggccg cgaggatacg ctttcccctg
     1141 gtacgcctct cggccaacgg ccaggccaga agttctatcg gccccgcgaa ccatgccatc
     1201 aagcttcagc tccgacgggg tgaaaggggc gggactgcgc caggcgcgct gccaccccat
     1261 gggcggggct ctacgcggcc ccatgggcgg ggctctacgc cgcccgctag gaggagtgca
     1321 tgcaaaaact tgccgtctgt gcgtcggggt tcctcctctt cgccgtcctc ctggggtgtc
     1381 agacaacgcc aacgcaggcc ccgcggagcg ccatcctgct ccaatcggga ggggccgaac
     1441 gcgacgggac gcaggggcct tcggtcgtag agctgggcct ctcgttcggc ggcgcggacc
     1501 tggtcaaaag ctggaaggtg gacatcgcca acgcggaggg gagcgtgaga agctggaccg
     1561 gcgacgggaa gcgccttcca acgacgctga gctgggacgg caagagcgat gccggggcgc
     1621 cggcgcccga aggttcctac gtcgcacggc tgtcgatcga ctatcagaag acctacgagg
     1681 cggcttttgc cgagagcgct ggcttcgccc tcgatatcac gccgcaaggt gacatggcgc
     1741 cgttcgacgc gtcgcggctg gcacccggag tctatccttc ctggccggcc tccagcgcag
     1801 ccaagaccgc cggcttctct ccgagaagcg acacccttgc gcgaacggag aagatcgccc
     1861 tctcgttcgg gcaggcttcc gcggtcacag actggaagct taccatcgcc cagccggggc
     1921 aggcggcggt ccgcacgttc cagggggacg ggacgaggct tcctgccgcc gtgatctggg
     1981 acggacggag cgacgacgga acggcggccc cggatgggac gtacacggcg cagctctcgg
     2041 tggtctacgg gagcgcatct cagccggtgc aggtgaccag caaacccttc gtgctggatg
     2101 tgacgccgcc gagcggcgcc gtggtggccg atccgccggc cctcaccgcc aacgagggcg
     2161 gggagatcca ggcggagagc ttctccctgt cggcctcctc gaaagtcgct tcgatccaga
     2221 gctggaccct cagcgtgata gggcccgaca acaagccggt ggccagcttc agcgggctgg
     2281 aacccgacgc ccacgtcccc tgggatggaa agctcgccaa cggcgcttcg gtcgatcccg
     2341 cccagacgta ctccgtgctt gcccaggtgc aggatgcgta tggaaacgtg ggagtcatcc
     2401 agggctcgct gaacaggatc gcggtggcat ccgtcgagag cgctccgccg gtcagggatc
     2461 cagccggcgg caaacagccg gcaagtccca agcagccgga gatcgcgtcg cccgctgcca
     2521 cggaggagcc atccgccatc ccagaactgg ccggcttctc tcctcagagc gacacccttg
     2581 cgagaatgca gacgatcgca ctttcctacg ggcaggcgtc cctggttacg gcgtggaacc
     2641 tcgccatcgc ccagtcggga cgagccgccg cccgctcctt ccagggagac gggacgaagc
     2701 tgcctgcgtc cgtggtctgg gacgggctct gcgatgatgg gacgccggca cccgaaggga
     2761 cgtacacggc tcagctttcg gtgagctatc gggacgcgtc ccggccggcg ctggccacga
     2821 gccagccctt cgtgctggac gtgaccccgc cgaagggatc gatatccctt tccgctcccc
     2881 tgttctctcc ccgagagggg gccgatacgc tcacgctgag cctcgcggcg cgctcatcga
     2941 ccgccaggat cgatagctgg tccatggaca tctacgatcc ggacggcaat ctgttcaaca
     3001 gcctccacgg ccagtggccg accagccagg ccgtgtggga cgggaaaggc ctcggaggag
     3061 cgcggatcga atcggccgag agctacccgg tggttgccag gatccgagat gagttcggca
     3121 acgtggggga agccaagatc atcgttcccg tcgacatcct ggtggaaaag acggctgctg
     3181 gcaactgcat tctgacctcc aacatctact tcaaggactt caccgcggac tgccagcacg
     3241 tggccccgga tctcgcaaaa cagaacgtcg cccgcctgga tgccctggcg aagaagctga
     3301 agaagttccc ggactacaag atcaggatcg tcgggcacgc tgtcgcgatc tactggttca
     3361 accccgcgct ggccaagcgg gagcagcgcg atgtcctgat cccactatcc aaagcacgag
     3421 cggaggccat caagagagcc ctcgtggccc gggggatcaa cggcgcaaaa ctctcgaccg
     3481 aaggcgtcgg ggcttcggac cagtctgtgt ccgaccggaa cctggctgac cgatggcaga
     3541 atcgccaggt cgacctgtat gtcgagaagt gagctggtga atcgtgggtt ttttagcgat
     3601 ttgggaaaaa gggtctaaac tttggggtac aaaaggcagg gataaaccat gaaatcaagg
     3661 ggattcatag cgctcttggt cgccctggcc ggagtcgccc tcttgagcgg ctgcaagccg
     3721 gaccccgtgg cgaccgggat ggcaagcctg gactgctcct catgccatac ggggagcgca
     3781 acctccacct acgggctgca gatgctcgca aacgaggggg gatatgccaa ctcgggccac
     3841 tacaacggca tacgggagct caacactgca gccgcggtga cgagctcctt ggagctgtac
     3901 gagttctccg gaagccacgc aatgaacgaa aacagcgcca actgctcgca gtgccacacc
     3961 gcgcagggct tcctgacctg gttgagcacc ggcaccgccg gagtccaggc gtcctccagc
     4021 ccgccagggt gtttcacct
//
LOCUS       DASRZS010000040         3842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2717080, whole genome shotgun sequence.
ACCESSION   DASRZS010000040 DASRZS010000000
VERSION     DASRZS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2717080"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1545)
                     /locus_tag="VFH83_01010"
     CDS             complement(127..1545)
                     /locus_tag="VFH83_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET6484964.1"
                     /translation="MTLAVGVPLIGSLLLPLAGAASARLRNGLALAFVLVPLACTIGL
                     APAAVAGTTTALKVSGPLGFDFILKADTLAVLMAFVSSLVGAIIVLYSFDYISHYGNQ
                     NEYYLMAVLFIGAMMGLVFSANLIFLFVFWEITGIASWRLIGFYRKPADVLRADKAFL
                     VTTFGALVMLIAIIAIDGTFGSFDLDAMTGSALPAGLAVLLLVGILSKSATLPFHTWL
                     PDAGVAPSPVTALLHAAVLVKIGVYVYARLFLVVFSTPEAVRTAVMVVAAASALVSAG
                     AALVENDLKRIIAFSTVSQIGFIFLGLASGTAMGAAGALLYILAHGLAKGGLFLTAGI
                     VEQNAGSKDIRQLGGIIRTMPVTAISFLLCAFSIMGIPPFGGFFSKFMVVSGAVGSGQ
                     IALAAVFMGGAVLTILYLSRLFLRVFTGEPRAGRLAPERSPAMVACVVTLAVLSLASG
                     LAVGLLSGTLNSAVAQMAMVGK"
     gene            complement(1697..3742)
                     /locus_tag="VFH83_01015"
     CDS             complement(1697..3742)
                     /locus_tag="VFH83_01015"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6484965.1"
                     /translation="MPGQLFTPASFGRLTIPNRLVRSATIECMCPEPGVLSDAYLSLY
                     EDLARGGAGLIVTGNYFVHPKGVAQPGILVLDSDAVIPQLGRLVAVVKRHGALIFGQL
                     NHGGRFCKPDFVGGTPMAPSPVYERINRVVPRPMTEEDIQTAIEAFASAAVRLERAGF
                     DGVEINAAHGYLVNQFLASRTNRRKDSWGGSLQNRFRFLERIVTRIRECTRFPVSVKL
                     NGRDYVRGGVTLEQSILLAQRLEALGVGGVTVSGGMKDSPFTTMDRGDVPSALIVSGR
                     RGFDRLRGLLFVRIQRGGAEFTEGYFLEDAAAIRRSVSIPVTAVGGFRSKAVMESALE
                     KVDFIGLSRPFIREPDLPRRLREGASESSSCMNCNLCLIRTVLRYDPLNCYWKRGAER
                     QRGGARETAEIREERLTTSDRARIYYRSLVPARKRAAVLFLHGMSEHSGMYPHVIRSL
                     GAAGFLVLAPDQRGRGQSVSRRWRRGDLHSIQRVLSDLDELRRHRSAELDGLPLFVLA
                     ISMGTVIAQRYALERPGVFSGMVLVAPPFGVPSGTSPLMLSLSGLLAAVTPRLPVRPA
                     PAIKAISRERVFQWELECDPYCYHGPLRARAGRELVSTLLLLQDRLKEIRLPLLIQYG
                     TGDRIVSRREPEEIRDRWGGQDRTLSVYEGLYHDVLNEPERQQAIDQIVTWIGTRAG"
BASE COUNT          727 a         1274 c         1269 g          572 t
ORIGIN      
        1 gtcccaacgc cgcgaagact acggtagcca ggttccgcgc aaggcccgcg gccttgggaa
       61 gcaggagaat caccgctgcc cccaggacgg gcagcccgac agggaggaga agccaggcag
      121 gatcgctcat ttgcccacca tcgccatctg ggccaccgcg gagttgaggg tgccagagag
      181 gagacccacg gccagaccgc tcgccaggga gagtacggca agcgtcacca cgcatgccac
      241 catggcggga gagcgctccg gcgcaagcct gcccgcgcgg ggctcacccg taaacacccg
      301 gagaaagaga cgggagaggt acaggatggt gaggacggct ccccccatga acacggccgc
      361 cagagcgatc tggccggatc ccacggcccc ggagaccacc atgaacttgc tgaaaaaccc
      421 gccgaatggg ggaatcccca tgatggagaa ggcgcacagc aggaaggata tcgcggttac
      481 cggcatcgtc ctgatgattc cgccgagctg gcggatgtcc ttgctgcccg cgttctgctc
      541 gacgatcccc gcggtaagga agagaccgcc cttcgccagt ccgtgggcca ggatatagag
      601 gagcgccccc gccgcgccca tcgcggtccc ggaggccaga cccaggaaga tgaatccgat
      661 ctggctcacc gtggagaacg cgatgattcg cttcaggtcg ttctcgacca gggccgcccc
      721 cgcgctgacc agggcgctgg ccgcggcaac gaccatgacg gccgtccgga ccgcctccgg
      781 tgtgctgaag accaccagga agagccgcgc gtacacgtac accccgatct tcaccaggac
      841 cgcggcgtgc aggagcgcgg tcaccggaga cggcgccacg cccgcgtccg gcagccaggt
      901 gtggaagggc agcgtcgcgg acttggagag gatgccgacc agcagaagca cggcaaggcc
      961 cgccgggagc gcactgcccg tcatggcgtc caggtcgaaa gacccgaagg tgccatcgat
     1021 ggcgatgatc gcgatcagca tcaccagggc tccgaaggtg gtgaccagga aggccttgtc
     1081 ggcccgaagc acgtctgccg gctttcggta gaagccgatc aggcgccagc tcgcaatccc
     1141 cgtgatctcc cagaacacga acagaaagat caggtttgcc gagaacacga ggcccatcat
     1201 ggcgccaatg aagagcaccg ccatcaggta atactcgttt tggttgccgt aatggctgat
     1261 gtagtcgaag gagtacagca cgatgatggc gcccaccagg gacgagacga acgccatcag
     1321 caccgccagc gtatccgcct tgaggatgaa atcgaatccc aaggggccgg ataccttcag
     1381 cgccgtggtc gtccccgcca cagcagcggg cgcgaggccg atggtgcagg ccagggggac
     1441 caggacgaag gccagcgcaa gcccatttct gagcctcgcc gacgcggcgc ctgccagggg
     1501 aaggaggagg gagccaatca gcggcacccc aacggccaga gtcatgagtt ttgctgaatc
     1561 catcgctttt tggggctgcg gttcacgtag tggcggggat gggccgtcca gaaccagtcg
     1621 agccgagctt tgatagcata ttgaaccaaa ctcgtccaga gggcaggtac tctgcctgtc
     1681 gaggcctcgg ggccgcctat cccgcccgcg tgccgatcca ggtgacgatc tgatcgatgg
     1741 cttgctggcg ctcgggctcg ttcaggacgt cgtggtagag gccctcgtag acggagaggg
     1801 ttcggtcctg gcctccccag cgatcgcgga tctcctcggg ctcccttcgg gagacgatcc
     1861 ggtcccccgt gccatactgg atgaggagcg ggagccggat ctccttcagg cgatcctgga
     1921 ggaggaggag ggtactcacg agctctcgac cggctcgcgc gcgcagcggg ccgtggtagc
     1981 agtagggatc gcactccagc tcccactgga acacccgctc ccggctgatg gccttgatgg
     2041 cgggagcagg gcggacgggc agccgcggcg ttacggccgc caggagaccc gatagggaga
     2101 gcatcaaggg cgaggtgccg ctgggcaccc cgaagggggg cgccacgagg accattccgg
     2161 agaaaacgcc gggtctctcc agcgcgtatc gttgggcgat gaccgtgccc atgctgatcg
     2221 ccaggacgaa gagcgggaga ccgtccaact cggcgcttcg gtgccgccga agctcgtcca
     2281 ggtccgatag cacccgctgg atcgagtgca ggtccccccg ccgccaccgt cgagacacgg
     2341 actggccccg ccctctctgg tccggtgcca gcaccaggaa ccccgcggcg cccagggagc
     2401 ggatcacgtg ggggtacatc cccgagtgct cactcatgcc gtggaggaag aggaccgccg
     2461 cgcgtttccg cgccgggacc agagaccggt aatagatccg cgcccggtcg gaggtggtga
     2521 gcctttcctc gcggatctca gcggtctctc gtgcgccccc tcgctgtctc tcggctcccc
     2581 gcttccagta gcagttcaag gggtcgtacc gcagaaccgt ccggatcagg cacagattgc
     2641 agttcatgca ggaggacgac tcggaagcgc cctcccgcaa gcggcgaggc agatccggtt
     2701 cgcggatgaa cgggcgcgag agcccgatga agtccacctt ctccagcgcg ctctccatca
     2761 ccgccttgga tcggaagcct ccgaccgcgg ttaccgggat ggagacgctc cgacggatgg
     2821 ccgccgcatc ctccaggaag tacccctccg tgaactcggc cccgcccctc tggatgcgga
     2881 caaagagaag accccggagc cggtcgaacc cccggcgccc ggagacgatc agtgcgctcg
     2941 ggacatcccc ccggtccatg gtggtgaagg ggctgtcctt catcccgccg ctcaccgtca
     3001 ccccgcccac acccagtgcc tccagcctct gtgccaggag gatgctctgc tccagggtga
     3061 cgccgccgcg cacgtagtcc cggccgttca gctttacgct taccgggaac cgcgtgcatt
     3121 cgcggatccg ggtcacgatc cgctccagga accggaaccg gttctgaagc gagcctcccc
     3181 aggagtcctt ccttcggttg gtcctggagg ccaggaactg gttcaccagg tagccgtggg
     3241 cggcgttgat ctccacgccg tcgaaccccg cccgctccag gcgaacggcc gcggaggcga
     3301 atgcttctat ggcggtctgg atgtcctcct cggtcatggg ccgcggtacc acccgattga
     3361 tccgctcgta gaccggggaa ggggccatgg gcgtcccgcc cacgaagtcc ggcttgcaga
     3421 acctgccacc atggttgagc tggccgaaga tgagggcgcc gtgacgcttg acgaccgcaa
     3481 cgagcctccc cagctgcggg atcaccgcgt cggagtccag gaccaggatc cccggctggg
     3541 ccacgccctt ggggtgaacg aagtagtttc ccgtcacgat caggcccgcg ccgccacggg
     3601 ccaggtcctc gtacaggctc aggtaggcgt cgctcaacac ccccggctcc ggacacatgc
     3661 actcgatggt agcggatcgg acgagccggt tcgggatcgt gaggcgacca aaggacgcag
     3721 gggtgaaaag ctgcccaggc atcttctctc cactttggcg gattatggcc gcaagtatgg
     3781 tcgatttctg aaagtttgca aggagcgatc cggcggcccg gcgcctctct tgacggacca
     3841 tg
//
LOCUS       DASRZS010000041         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1950254, whole genome shotgun sequence.
ACCESSION   DASRZS010000041 DASRZS010000000
VERSION     DASRZS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950254"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2336
                     /gene="pnp"
                     /locus_tag="VFH83_01020"
     CDS             <1..2336
                     /gene="pnp"
                     /locus_tag="VFH83_01020"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET6484966.1"
                     /translation="GEEELVLETGKMAKQANGAVFGRLGGSAVIATACAAENEKEDLD
                     FIPLSVDYIEKYYAAGKIPGGFIKREGRPKDKEILVSRLIDRPIRPLFSRLFRREIQV
                     IPMAISTDLVNPPDILAMNAASAAITISDIPFAGPIGAVRMGYVDNRLIVNPTFDQIQ
                     ETKLELVVAGTSKGITMVEGGARQVSESLIIEAIEMAHRTIMELCRIQLELAQKAGKQ
                     KLPLVERPSTFTLGSEVRSWSMPKLQEANFVKGKENRSRAIKAVRTECIEHFKDRLAE
                     TDIKPVKLLFEDMEQEIMRESIANRGIRTDGRTPTQIRPITCEIDLLPRTHGSALFTR
                     GETQALVVTTLGTALDEQIMDNIDGDSRKGFMLHYNFPPFSVGEVGRLGSPGRREIGH
                     GHLAERAISNVLPPKENFPYTVRIVSEILESNGSSSMATVCGSTLSLLNAGVRILKPV
                     AGIAMGLVKEGEKFVVLSDILGEEDHLGDMDFKVAGTEDGITALQMDIKIEGVNAEIL
                     TKALGQAREGRLHILGIMNRTISEPRADISDFAPKVISVKVPTEKIGLIIGPGGKNIK
                     GMSEKTGSQINIDDDGTVTIYNRVKEGAEKAQKMVEAMIEEAVVGKTYTGPVKRIMDF
                     GAFVEILPGKEGLVHISKLAATRVENVNDVVKEGDVLTVTVTEIDHMGRINLATKEAL
                     EANPGLMTERAERRPPRRDGDRPRGGYRDGPRRDGPRYDGPRHDGPRGGHDGPRSDRH
                     PGGYSDRPRRGDGPRGDGPRRDGPPGGHDSPQGGFEE"
     gene            2333..>3285
                     /locus_tag="VFH83_01025"
     CDS             2333..>3285
                     /locus_tag="VFH83_01025"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6484967.1"
                     /translation="MIYTERLASGATLLLEPVDRTDTLCIGFWFLHGSRDESPGQRGF
                     SHLLEHMLFKGTGKRSALAIAQEIDRVGGIINAFTEKETTCVYTIIPREHLRLAFDIL
                     SDMIVGSLLDPTEMDKEKEVIITEIRSIDDSPEEKGHDRYLREMWGDHALAQKITGEV
                     QDVRGAGREALAQFGRQWLVPANTIIAVAGNFEPQEARELASAVYTGAAPMARGPRTA
                     PTWRRRTAFVADRFNQVQIYAGTSYGLDHQVGPYYTSLVFSTAVGESMSSRLFQRLRE
                     DLALCYTVYSFRTFFSDTGLWTIYGNTTPAQTRKFLEELDR"
BASE COUNT          649 a         1071 c         1100 g          465 t
ORIGIN      
        1 tcggggagga ggagctcgtc ctggaaacgg gcaagatggc caagcaggcc aacggcgccg
       61 tgttcggccg gctcggaggc tcggcggtca tcgcgaccgc ctgtgccgcg gagaacgaga
      121 aagaagacct ggacttcatc cccctctccg tcgactacat cgagaagtat tacgccgcgg
      181 ggaagatccc cggcggcttc atcaagcgcg agggcaggcc gaaagacaag gagatcctgg
      241 tctcgcggct tatcgatcgc cccatccggc cgctgttctc caggctgttt cgcagggaga
      301 tccaggtcat cccgatggcc atctccacgg acctggtcaa cccaccagac atcctggcca
      361 tgaacgccgc atccgcggcc atcacgatct ccgatatccc gttcgcgggg cccatcgggg
      421 ccgtgcgcat gggctacgtg gacaaccggc tgatcgtcaa cccgaccttt gaccagatcc
      481 aggaaacgaa gctggagctg gtggtggccg gcacctccaa gggcatcacc atggtggaag
      541 gcggggcccg ccaggtctcc gagagcctca tcatagaggc aatcgagatg gcgcaccgca
      601 ccatcatgga gctgtgccgc atccagctcg agctggccca gaaggccggc aagcagaagc
      661 tcccgctcgt ggagaggccg agcacgttca ccctcggaag cgaggtccga agctggtcca
      721 tgccgaagct gcaggaagcc aacttcgtga agggcaagga gaaccgcagc cgggccatca
      781 aggccgtccg cacggagtgc atcgagcact tcaaggaccg cctggcggaa acggacatca
      841 agcccgtgaa gctcctgttc gaggacatgg agcaggagat catgcgggag tcgatcgcca
      901 accgcggcat ccgcacggac ggcaggaccc cgacgcagat ccgccccatt acgtgcgaga
      961 tcgacctgct tccgcgcacc cacggctccg cgctgttcac tcgcggcgag acccaggcgc
     1021 tggtggtcac cacgctgggc acggcgctgg acgagcagat catggacaac atcgacgggg
     1081 acagccgcaa gggcttcatg ctgcactaca acttcccgcc gttctcggtg ggcgaagtgg
     1141 gcaggctggg atccccgggg cggcgcgaga tcgggcacgg ccacctggcc gagcgcgcaa
     1201 tctccaacgt gctgccgccg aaagagaact tcccctacac ggtgcgcatc gtctccgaga
     1261 tcctggagtc caacgggtcc tcgtccatgg ccacggtgtg cggaagcacg ctcagcctgc
     1321 tcaacgcggg cgtgcggatc ctgaagccgg tggcgggaat cgccatgggc ctggtgaagg
     1381 aaggcgagaa gttcgtcgtc ctgagcgaca tcctcggaga ggaagaccac ctcggggaca
     1441 tggatttcaa ggtggcgggc accgaggacg ggatcaccgc cctgcagatg gacatcaaga
     1501 tagagggcgt gaacgcggag atcctcacca aggcgctggg ccaggcccgc gaggggcggc
     1561 tccacatcct cgggatcatg aaccgcacca tctccgagcc gcgggccgac atctcggact
     1621 tcgcgcccaa ggtcatctcc gtgaaggttc ccaccgagaa gatcgggctg atcatcggcc
     1681 cgggcggaaa gaacatcaag ggaatgagcg agaagaccgg ctcgcagatc aacatcgacg
     1741 acgacgggac ggtcacgatc tacaaccgcg tgaaagaggg cgccgagaag gcgcagaaga
     1801 tggtggaggc catgatcgag gaggcggtgg tgggcaagac ctacaccggc cccgtgaagc
     1861 gcatcatgga cttcggcgcg ttcgtcgaga tcctccccgg caaggaaggg ctggtgcaca
     1921 tctccaagct ggccgccacc cgggtggaga acgtcaacga cgtggtgaag gagggggacg
     1981 tgctcaccgt gacggtcacg gagatcgatc acatgggccg gatcaacctc gccaccaagg
     2041 aagccctgga agccaatccc ggtctcatga cggagcgcgc cgagcggcgg ccccctcgcc
     2101 gggacggaga ccggccccgc ggcggctacc gcgacgggcc gcggcgagac ggtccccgct
     2161 acgacggacc acgccacgac ggaccgcggg gcggtcatga cggcccccgc tcggaccggc
     2221 accccggcgg ctattccgac cggccccggc gcggggatgg cccgcgaggg gacggacccc
     2281 gccgggacgg acccccgggc gggcacgact ccccccaggg cggtttcgag gagtgattta
     2341 cacggagaga ctggcctcgg gggcaacgct gctcctcgag ccagtcgacc gcaccgacac
     2401 cctctgcatt gggttctggt ttctgcacgg ctcgcgtgat gaaagccccg gacaacgggg
     2461 cttttcccat ctgctggagc acatgctgtt caagggcacg gggaagcgct ccgcgcttgc
     2521 catcgcgcag gagatcgacc gggtgggcgg catcatcaac gccttcacgg agaaggaaac
     2581 gacctgcgtc tacaccatca ttccccggga gcacctgcgg cttgcgttcg acatcctctc
     2641 cgacatgatc gtcgggtccc tcctggaccc gacggagatg gacaaggaaa aggaagtcat
     2701 catcaccgag atccgctcca ttgacgactc gccggaggag aaggggcatg accgttacct
     2761 ccgcgagatg tggggggatc acgccctggc gcagaagatc accggcgagg tgcaggatgt
     2821 gcgcggggcc ggccgcgagg cgctcgccca gttcggccgg cagtggctcg tgccggccaa
     2881 cacgatcatc gccgttgccg gcaatttcga gccccaggag gcgcgcgagc tggcctccgc
     2941 ggtgtacacg ggagcggcgc cgatggcacg gggcccgcgc acggcgccga cctggcgccg
     3001 caggacggcg ttcgttgcgg accggttcaa ccaggtccag atctacgcgg gcaccagcta
     3061 cggcctggat caccaggtcg ggccgtacta cacctccctg gtcttcagca cggccgtggg
     3121 agagtccatg agcagccggc tgttccagag actgcgagag gacctcgccc tgtgctacac
     3181 cgtgtactcg tttcggacct tcttctctga cacgggcctg tggaccattt acggaaacac
     3241 cacgccggcc cagacgcgga agttcctgga ggagctggac cgcga
//
LOCUS       DASRZS010000042         3146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5029118, whole genome shotgun sequence.
ACCESSION   DASRZS010000042 DASRZS010000000
VERSION     DASRZS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5029118"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..922)
                     /locus_tag="VFH83_01030"
     CDS             complement(29..922)
                     /locus_tag="VFH83_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6484968.1"
                     /translation="MTVSDMEGKRPSAKLFEAVLILFMVLFGITVLYPFVFLLALSFN
                     QGLDALKGGITLWPRKVTLENYSIIVKDPRLLHAVFISVSRTALGTFLAVLITGYVSF
                     CFTKRQMVGRRFYMTLLILPMYINAGIIPTYLTYRAFGLTNTFLVYIVPNLVWAYNIL
                     IMRTFFQSLPPAFEESAMIDGANEFVVFFRVVFPLSMPVVATIGLFNAVWHWNTWFDT
                     ILLTRKTSLETLMSLLAKMLLEQQSNLISDLRNARRVRFMTPTVLRAAMTIVTTVPIV
                     LVYPFLQKYFVKGVLIGGVKG"
     gene            complement(933..1868)
                     /locus_tag="VFH83_01035"
     CDS             complement(933..1868)
                     /locus_tag="VFH83_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6484969.1"
                     /translation="MTLPPPTSRLGRLWWEFKRQRNLQIVMVPVALWTIAIFYLPMLG
                     NVIAFQDYEVYQGFTGSPWVGLKHFIAFFTNRFTLVLIRNTLAMSMLSLVVGTLAAIL
                     FALLLNEIRSLAFKRVIQTISYLPYFVSMAVCANLFVQLLGRIGALNDILMGARVLSE
                     PFPFLEKPGLFWGILTLQGVWKNVGWNAIIYLAAISGIPEEIYEAAYIDGAGRARRMW
                     SITLPSILPTIAVLLIMNSGYLIMGGFEQQLLMLNPMVLDVGEVLQTYVYKRGISGAQ
                     YSFAAAVGLFQSAVSILIILLVNRASKGLADIALW"
     gene            complement(1872..>3146)
                     /locus_tag="VFH83_01040"
     CDS             complement(1872..>3146)
                     /locus_tag="VFH83_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484970.1"
                     /translation="LGHVFNYWKYDDGKIYRMRGWVWNDPRYSLSFDVNTLYMRYDVL
                     KELGYPKLSRDKSLNSIITIDEYMNILNQVRQKHPEMTPVLMDGWFAAYTMLASTGMS
                     DIGDAVWENGQAKYIFDSKDTAWAIQWLNKLYLDGYVEKGFATLSKEQFQALASSGKV
                     FSTLGRFEGLPEARSSLSAETDEKRLAMFYLTKDATVKHVGINGYYIDGDDSVFITTK
                     AKDPVGIMKFFDWCNTEDGLVLLNAGVEGLSWNWKDNRRVPTDKAMKAYYLWDSTLLK
                     ELGLGTWNGFLPSLAGMDKTGNAYDINQQATFESNKWGQYDMTDWKFFANPNYASEKV
                     AFIDSVKQKDAYDANSKIQAYLRDRITKAIAAPSAADSASQWKDTYAMMKADGLDALN
                     AAKNENWLKAAKFLNKQPVDLLKDVAVIGASD"
BASE COUNT          605 a          925 c         1011 g          605 t
ORIGIN      
        1 ggatccgctc tctccgcgtc acggcgccct atcctttcac gcctccgatg agcactccct
       61 tcacgaagta cttctggaga aacgggtaga cgagcacgat cggcacggtg gtgacgatgg
      121 tcatggcggc ccggagcacc gtgggggtca tgaagcgcac tcgtctggcg ttgcgcaggt
      181 ccgagatgag attggactgc tgttccagga gcatcttcgc cagaaggctc atgagggttt
      241 ccagagaggt cttccgcgtg agcaggatgg tgtcgaacca ggtgttccag tgccacacgg
      301 cgttgaagag gccgatcgtg gccactaccg gcatcgagag cgggaagacc acccgaaaga
      361 agaccacgaa ctcattagcg ccatcgatca tcgcggactc ctcgaacgcg gggggcaggg
      421 actggaagaa ggtccgcatg atcaggatgt tgtaggccca tacaaggttg gggacgatgt
      481 acaccaggaa cgtgttggtc agcccgaagg cccggtaggt caggtaggtt gggatgatac
      541 cggcgttgat gtacatcggg aggatgagga gcgtcatgta gaaccggcgg cccaccatct
      601 gccgcttggt gaaacagaac gagacgtagc cggtgatcag gacggccagg aaggttccca
      661 aagccgtgcg ggacacggag atgaacaccg cgtgcagcag gcgcggatcc ttcacgatga
      721 tggagtagtt ctccagggtg accttgcggg gccagagggt gatcccgccc ttcagggcgt
      781 ccagtccctg gttgaaggac agggcgagca ggaacacgaa cgggtagagc acggtgatgc
      841 cgaagaggac catgaagagg atgaggaccg cctcgaagag cttcgcggag ggccgcttgc
      901 cttccatgtc cgaaacggtc acgccctcct cctcaccata gcgcgatgtc cgccagccct
      961 ttgctggcgc ggttcaccag gaggatgatc aggatgctca ccgcggactg gaaaagcccg
     1021 accgccgcgg cgaacgagta ctgggcgccg ctgatgcctc gcttgtagac gtaggtctgg
     1081 aggacctccc cgacgtccag gaccatcggg ttgagcatca ggagctgctg ctcgaatcct
     1141 cccatgataa gatagcccga gttcatgatg agcaggaccg caatggtggg caggatgctg
     1201 ggaagcgtga tggaccacat ccgccgcgcg cggcccgccc cgtcgatata cgcggcctcg
     1261 tagatctcct ccggaatccc cgagatggcg gccaggtaga tgatcgcgtt ccaccccacg
     1321 ttcttccaga cgccctggag cgtgaggatt ccccagaaga ggcccggctt ttccaggaac
     1381 gggaacggct ccgagaggac ccgcgccccc atgaggatgt cgttcagggc accgatgcgc
     1441 cccaggagct ggacaaagag gttggcgcag accgccatgg aaacgaagta tggcaggtag
     1501 gagatggtct ggatgacccg cttgaaggcc aggcttcgaa tctcgttcag gagcagggca
     1561 aagaggatgg ccgccagagt cccgaccacc agggagagca tgctcatggc cagggtgttg
     1621 cggatcagga ccagcgtgaa ccggttggtg aagaacgcga tgaagtgctt gagcccgacc
     1681 caggggctgc cggtgaagcc ctggtagacc tcgtagtcct ggaacgcgat gacgtttccg
     1741 agcatgggca gatagaagat cgcgatggtc cacagggcta ccgggaccat cacgatctgg
     1801 agattcctct ggcgcttgaa ctcccaccac agcctgccca gccgggaggt ggggggcgga
     1861 agcgtcacga ctcagtccga cgcgccgatg accgccacgt ccttcagaag gtccacgggc
     1921 tgcttgttca ggaacttcgc cgccttgagc cagttctcgt tcttggccgc gttcagcgcg
     1981 tccagcccgt ccgccttcat catggcgtag gtatccttcc attgagaggc ggaatccgcg
     2041 gcgcttggcg cggcgatggc cttggtgatc cgatcgcgca gatacgcctg gatcttcgag
     2101 ttggcgtcgt aggcgtcctt ttgcttcacg gagtcgatga acgcgacctt ctcgctggcg
     2161 tagttggggt tggcgaagaa cttccagtcc gtcatgtcgt actggcccca cttgttgctc
     2221 tcgaaggtgg cctgctggtt gatgtcgtag gcgtttccgg tcttgtccat gcccgcgagg
     2281 gacggaagga acccgttcca ggtccccagc cccagctcct tgagcagcgt ggaatcccag
     2341 aggtagtagg ccttcatggc cttgtcggtg gggaccctcc ggttatcctt ccagttccag
     2401 ctcaagccct cgacgccggc gttcaggagc accagcccgt cctccgtgtt gcaccagtcg
     2461 aagaacttca tgatgccgac cggatccttg gctttggtgg tgatgaacac gctgtcatcc
     2521 ccgtcgatgt agtagccgtt gatccccacg tgcttcacgg tggcgtcctt ggtcaggtag
     2581 aacatggcca ggcgcttctc gtcggtctcg gccgacaagg aggagcgcgc ctccggcagg
     2641 ccttcgaacc ggcccagggt gctgaacacc ttgccgctgg aggccagggc ctggaactgt
     2701 tccttgctga gcgtggcgaa gcccttctcc acgtagccgt ccaggtagag cttgttcagc
     2761 cactggatgg cccacgccgt gtccttgctg tcgaaaatgt actttgcctg gccgttctcc
     2821 cagaccgcgt cgccgatgtc gctcatcccc gtggatgcaa gcatcgtgta cgcggcgaac
     2881 cacccgtcca tcagcaccgg cgtcatctcg ggatgcttct gccgtacctg gttcaggatg
     2941 ttcatgtact cgtcgatcgt gatgatcgag ttgagggact tgtcccgaga cagcttgggg
     3001 tatcccagct ccttgagcac gtcgtagcgc atgtacaggg tgttcacgtc gaagctcagg
     3061 gagtaccggg ggtcgttcca gacccacccg cgcatgcggt agatcttccc atcgtcgtac
     3121 ttccagtagt tgaacacgtg cccgag
//
LOCUS       DASRZS010000043         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1951193, whole genome shotgun sequence.
ACCESSION   DASRZS010000043 DASRZS010000000
VERSION     DASRZS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951193"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VFH83_01045"
     CDS             <1..922
                     /locus_tag="VFH83_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6484971.1"
                     /translation="KLPPGDYVTTRIQQLEQSGGPVNDSELKSLRHQYGLDQPTTVQY
                     LKWIWNMFHGDLGRSFMWDRPVTELIGERLVLTLVISLLSLLLSYLLAIPIGIYSATH
                     QYSFGDYAFTVFGFVGIAIPGFLIALILMFLFYKYFGVSVTGLFSQGFQQAPWSWGKV
                     LDLLKHLPIPVVVVGMSGTAYLIRVMRGCLLDELKKQYVVTARAKGVSERALLFRYPV
                     RIALNPLVSTVGWILPQLISGETIVALILNLPTTGPLLFNALVGQDMYLAGSFIMILS
                     FFTVVGTFVSDMLLMVVDPRIRLEKQGGGV"
     gene            922..2130
                     /locus_tag="VFH83_01050"
     CDS             922..2130
                     /locus_tag="VFH83_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6484972.1"
                     /translation="MAAGNAAPRGADTRHAATVSPPGAHNAQEESYYVASQWKLIGRK
                     FRRHKLSMVSLCVLGLMYLGAILCEFLAPYDPAQRFERYLYCPPQRVHFIDASHRFHL
                     RPFTYGLKQTLDMVSLVRTYQEDTAVSYPVRFFVHGSPYKFWGLVRTSVHFFGVGDAS
                     GGGATATGDTSGGTIFLFGTDKLGRDLFTRNLAASRISLTVGLVGVAISFVLGSLIGG
                     VSGYFGGRLDTFIQRTIEILLSIPTIPLWMGLAAALPPDWPSIKVYFGITIILAIIGW
                     SGLARVVRGRLLELREEDFVMAARISGASSLSIIVRHLLPSFMSYLIVNLTLSIPYQI
                     LGETALSFLGLGLQPPVVSWGVLLHDAQNLATIAFHPWLLLPAVWIVVTVLMFNFLGD
                     GLRDAADPYR"
BASE COUNT          319 a          729 c          673 g          435 t
ORIGIN      
        1 caagctcccg ccgggggact atgtcaccac gcggatccag caactggagc agtccggcgg
       61 tcccgtgaac gactccgagc tcaagtccct ccgccaccag tacggcctgg accagccgac
      121 cacggtgcaa tacctcaagt ggatctggaa catgttccac ggggacctgg gtcgctcgtt
      181 catgtgggat cggccggtga ccgagctgat cggcgagcgc ctggtcctga ccctggtcat
      241 ctccctgctg agcctcctgc tctcctatct cctggccatc ccgatcggga tctactccgc
      301 cacgcaccag tactccttcg gagactacgc gtttaccgtg ttcggctttg tcggcatcgc
      361 catccccggc ttcctcatcg cgttgatcct gatgttcctg ttctacaagt acttcggggt
      421 cagcgtgacc gggctcttct cccaggggtt ccagcaggcg ccgtggagct ggggcaaggt
      481 cctggacctc ctcaaacacc tgcccatccc cgtggttgtc gttgggatgt ccggcaccgc
      541 ctatctgatc cgcgtgatgc ggggatgcct cctggacgag ctcaaaaagc agtacgtggt
      601 gaccgcccgg gccaaggggg tctccgagcg cgcgctgctc ttccgctatc cggtgcgcat
      661 cgccctgaat cccctggtga gcaccgtggg atggatcctg ccccagctca tctcgggaga
      721 gacgatcgtg gcgctgatcc tcaacctgcc aaccacgggg ccgcttcttt tcaacgcgct
      781 ggtcgggcag gacatgtacc tggccggaag cttcatcatg atcctcagct tcttcaccgt
      841 ggtcgggacg ttcgtgtcgg acatgctcct gatggtggtg gatccgcgga tccgcctgga
      901 gaagcaggga ggaggcgtct gatggccgcg gggaacgcag cgcctcgcgg cgcggatacg
      961 cgccacgccg ccaccgtgag ccccccgggc gcgcacaacg cgcaggagga gagctactac
     1021 gttgcctccc agtggaagct gatcgggagg aagttccgaa ggcacaagct ctccatggtg
     1081 agcctgtgtg tgttgggcct gatgtacctg ggcgccatcc tctgcgagtt cctggccccc
     1141 tacgatcccg cccagcgttt cgagcgttac ctctattgcc ctccgcagcg ggtgcacttc
     1201 atcgacgcgt cccaccggtt ccacctgaga ccgttcacct atgggctgaa gcagacgttg
     1261 gacatggtca gcctggtccg cacgtaccag gaggacaccg ccgtctccta cccggtccgc
     1321 ttcttcgttc atggcagccc ctacaagttc tggggcctcg ttcggaccag cgtgcacttt
     1381 tttggcgtgg gggacgcctc tggcggcggg gccaccgcca ccggtgacac ctctggaggg
     1441 accatcttcc ttttcggcac cgacaagctg gggcgggacc tgttcacgcg caacctggcc
     1501 gcttcccgga tctcgctcac cgtgggcctg gtgggagtcg ccatcagctt cgtcctgggc
     1561 tcgctgatcg gcggcgtctc tggctacttt ggcggccggc tggacacgtt catccagcgg
     1621 accatcgaga tcctcctgtc catcccgacg atccccctgt ggatggggct ggccgcggca
     1681 ctgccgccgg actggccttc gatcaaggtc tacttcggga tcacgatcat cctggcgatc
     1741 atcgggtgga gcggcctggc ccgggtggtc cgcggaaggc tcctggagct gcgggaggaa
     1801 gacttcgtca tggccgcgcg gatctccggc gcctccagcc tcagcatcat cgtccggcac
     1861 ctgctcccgt cgttcatgag ctacctgatc gtgaacctca ccctctccat cccgtaccag
     1921 atcctggggg agaccgcgct gagcttcctg gggctggggc tgcagccgcc cgtggtgagc
     1981 tggggcgtgc tgctgcacga cgcgcagaac ctggccacca tcgcgttcca tccgtggctc
     2041 ctcctgccgg ctgtctggat cgtggtcacg gtcttgatgt tcaacttcct gggggacggg
     2101 ctgcgggacg cggcggaccc ctaccgatga gcgccgtttc gatggcgggc gcggaa
//
LOCUS       DASRZS010000044         2816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1951577, whole genome shotgun sequence.
ACCESSION   DASRZS010000044 DASRZS010000000
VERSION     DASRZS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951577"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VFH83_01055"
     CDS             <1..922
                     /locus_tag="VFH83_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6484973.1"
                     /translation="DIYLPSMVESGQINGEQYSLPIDVHPIAMYYNVDMFKAAGLDPN
                     KPPKTYEELVAAAKKLTKGTSVYGVGLEQKNDIQFYGFMSFFYQNNGQLLTPDNKKSL
                     FDGAPARKTLAQLHDLIWKHKITPLGEEDARGDFRNKTVAIVFSGPWDNALESGFPSV
                     AGLNYRTAKVPQFGPTPATWESTHQIAVVKQKDKALEDTGAEFVKFFAENSIIWNQSG
                     MIPVLKAAAGDPRLAKLKEYSKGFMDSLSESKMMPIIPKGYIEMFGASPDRPVMQAIQ
                     AYLMDENPNADQILGDANKAISAILKRFYP"
     gene            1045..1911
                     /locus_tag="VFH83_01060"
     CDS             1045..1911
                     /locus_tag="VFH83_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6484974.1"
                     /translation="MKTRFQVIEPYLYIAPYFVVFILFLVAPAISGMVIGFYDWPVVG
                     DKHYLGLGNFQELFGDPMFIKSVLNTLEYSLMYMPIFMVGGLALALLLNRSFIGKSAM
                     RMIIYAPYVFMIPAIGVMWRWFLDTNYGILNYYLGVLHLGTVKWLTNPHVALKSIVMV
                     ISWEVLGYSMVIFLAGLQEIPAELYEAAEIDGASPAQKLWRITLPFLRPTAFFLFVIG
                     LIGALKTFGQPLMITAGGPLNSSMTVVMALFYQGFKYFRMGYAAAISALLFLGILIVT
                     LLQFRLIRQRNV"
     gene            1915..2754
                     /locus_tag="VFH83_01065"
     CDS             1915..2754
                     /locus_tag="VFH83_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6484975.1"
                     /translation="MSSRRTIGRIGLYLVCAAIALVSLFPTLWLIATSLKPERLIPHW
                     PPLWYAGALTLENYISVITKYDFLYWLRNSTIVSVFGTLLTIFLGSLASYAFSRFRFK
                     GRNLLFMLTVATVILPQEITIIPLYISLTRIGLGNTYLGLILPAIANPVSIFLFRQFF
                     DGFPTELEEAALMDGYGRFRIYYRLVMPLSTAVIMAVAILTFTAVWNDMLWPIIITSS
                     KRAATLPVGVVQHFEMFQGAKMMRYGDQSAASILCIIPTLLLFLFLQKFFVQGIARTG
                     IKG"
BASE COUNT          507 a          957 c          821 g          531 t
ORIGIN      
        1 ggacatctac cttccgtcga tggtggagag cggacagatc aacggtgagc agtactcgct
       61 gcccatcgat gtgcacccca tcgccatgta ctacaacgtg gacatgttca aggccgcggg
      121 gctggacccc aacaagccgc cgaagaccta tgaggagctg gtggctgcgg ccaagaaact
      181 gaccaagggc acctcggtgt acggcgtcgg cctcgagcag aagaacgata tccagttcta
      241 cggcttcatg tcgttcttct accagaacaa tgggcagctc ctcaccccgg acaacaagaa
      301 gtccctcttc gacggggcgc cggcccgcaa gaccctggcc cagctccacg acctgatctg
      361 gaagcacaag atcacccccc tcggggagga agacgcccgc ggcgacttcc gcaacaagac
      421 cgtggccatc gtgttcagcg gaccgtggga caacgcgctg gagagcgggt tcccctccgt
      481 ggcagggctg aattaccgca cggcgaaggt cccgcagttc gggcccacgc cggccacctg
      541 ggagtccacc caccagatcg ccgtggtcaa gcagaaggac aaggcgctgg aggacaccgg
      601 cgcggagttc gtgaagttct tcgcggagaa cagcatcatc tggaaccagt ccggcatgat
      661 cccggtcctc aaggccgccg ccggcgatcc ccgcctggcg aagctcaagg aatactccaa
      721 gggcttcatg gattcgctct cggagtccaa gatgatgccg atcatcccca aggggtacat
      781 cgagatgttc ggcgcgtccc cggaccggcc ggtgatgcag gccatccagg cctacctcat
      841 ggacgagaat cccaacgcgg accagatcct cggcgacgcg aacaaggcca tcagtgccat
      901 cctgaagcgg ttctatccat aagacgactc gcatgatgca atgggtgcgg gaccggcgcg
      961 cgccggtccc gcatcgcgca gggcgcacga ggagcgcccg ggggcgccgg aggagcgccg
     1021 gaaggcgctg aggaggccga gcgaatgaag acgaggttcc aggtcatcga gccgtatctg
     1081 tatatcgctc catatttcgt cgtcttcatc ctcttcctcg tcgcccccgc gatctccggc
     1141 atggtgatcg ggttctacga ctggcccgtg gtgggcgaca agcactacct cggcctgggc
     1201 aacttccagg agctgttcgg cgacccgatg ttcatcaagt cggtgctgaa caccctggag
     1261 tactccctca tgtacatgcc gatcttcatg gtgggcggcc tggcacttgc gctgctcctg
     1321 aaccgctcct tcatcggaaa gtcggcgatg cgcatgatca tctacgcgcc gtacgtcttc
     1381 atgatccccg ccatcggcgt catgtggcgg tggttcctcg acaccaacta cggcatcctc
     1441 aattactacc tgggcgtgct ccacctcggc accgtgaagt ggctcaccaa cccccacgtc
     1501 gccctgaagt cgatcgtcat ggtcatctcc tgggaggtgc tggggtacag catggtcatc
     1561 ttcctcgccg ggctccagga aatccccgcg gagctctacg aggcggcgga gatcgacggg
     1621 gcctcccccg cgcagaagct ctggcggatc acgctgccgt tcctccgccc gaccgccttc
     1681 ttcctcttcg tgatcggcct catcggcgcg ctgaagacct tcggccagcc gctgatgatc
     1741 acggcggggg gacccttgaa ctcgagcatg accgtcgtca tggccctgtt ctaccagggg
     1801 ttcaagtact tccgcatggg ctacgccgcg gcgatctccg ccctcctgtt cctgggtatc
     1861 ctgatcgtca cgctcctgca gttccgcctg atccgccaga ggaacgtcta ggccatgagc
     1921 agccgcagga cgatcggacg gatcgggctg tacctcgtgt gtgcggccat cgcgctcgtc
     1981 tccctcttcc cgaccctctg gctcatcgcc acgtcgctga aaccggagag gctcatcccc
     2041 cactggccgc ccctgtggta cgcgggagcg ctcaccctgg agaactacat ctcggtgatc
     2101 acgaagtacg acttcctgta ctggctgcgc aactccacca tcgtctccgt cttcgggacg
     2161 ctgctcacca ttttcctggg gtcgctcgcc agctacgcgt tctcccggtt ccgcttcaag
     2221 ggccgcaacc tcctgttcat gctcaccgtc gccacggtca tccttcccca ggagatcacc
     2281 atcatcccgc tctacatctc gctcacccgg atcgggctgg gcaacacgta cctgggcctc
     2341 atcctgccgg ccatcgcgaa cccggtgagc atcttcctgt tccgtcagtt cttcgacggc
     2401 ttccccaccg agctggagga agcggccctg atggacggct acgggcgatt caggatttac
     2461 taccggctgg tcatgccgct ttccaccgcc gtgatcatgg cggtggccat cctcacgttc
     2521 accgcggtct ggaacgacat gctgtggccc atcatcatca cctcctcgaa gagggcggcg
     2581 accctccccg tcggggtggt ccagcacttc gagatgttcc agggcgccaa gatgatgcgc
     2641 tacggcgacc agtcggccgc cagcatcctc tgcatcatcc cgacgctcct cctgttctta
     2701 ttcctgcaga agtttttcgt gcagggcatc gcgcggaccg ggatcaaggg atgacgggag
     2761 catgacatcc cgcgagcgtg tccggtcggt cctgaggggc gagaagcccg accgcg
//
LOCUS       DASRZS010000045         3135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5030013, whole genome shotgun sequence.
ACCESSION   DASRZS010000045 DASRZS010000000
VERSION     DASRZS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5030013"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..934)
                     /locus_tag="VFH83_01070"
     CDS             complement(26..934)
                     /locus_tag="VFH83_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484976.1"
                     /translation="MLGGARWDRVRRYPYRLTILVLSRGDRLFRAELLKELCEQDLGE
                     ILWVEGPDQPSDVESLARDFPQVRFLLLGARATAGEWVNVGIEEARAPQVLCLWSDTR
                     LSSFRPAILGDLESHHAVCVVPVSRNPRLEIIPSWQSPGWRRRRFLVTFRAPRKEGEP
                     TLYPFDYCGLYDRQRFAQSGGYDPAIQNPYWQRLDFGLRCLLWGERLCGTLQLTVTYT
                     GSPPTDDATPDQGYKLFFLKCVAVRLRREMGTLPRRRLLDYMIKSDTGPFYAIKEFRS
                     VRSWVWTHRFRFRKDPRQLVERWENG"
     gene            complement(960..2444)
                     /locus_tag="VFH83_01075"
     CDS             complement(960..2444)
                     /locus_tag="VFH83_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HET6484977.1"
                     /translation="MKNTAVINAISLGRHALLPLIDGASAVDRVTAFARSLPGVQDWV
                     MFLSRPLEAAAGMRTVIRDSWTQAELLAQMVDAAAGFEDVFYFYADCPFLDIELARRM
                     HENHRRYWADYTYADGYPYGLSPEILTRETASRLKAAASVAVPERDTLFSLISKDINA
                     YDIETELSPVDQRLLRVSLAADSQRNFLQLQRVVAAGARDAASVCDALQHNPEILRTL
                     PAYFTIQVVERCPHACSYCPYPVVAGDVMRKTGVMPTERFSDLAARISAFSGDAVIGI
                     SLWGEPALHPSIYDLISTVLETPSLRLVIETSGVGWDPSLFGRIKDGFQRQPTWIVSL
                     DATREDLYTQLRGKGFAEAVRSAETLLTLFPRSAWVQAVRMKENEDDLDVFYKSWKAR
                     TDNVIVQKYDPVGGLLADRRVADLSPLQRFPCWHLKRDLSILLDGTVPLCREDVRVTT
                     PLGNAFTEELSVIWERGQSVYRSHIARQYPGICAGCDEYYTYNF"
     gene            2577..>3135
                     /gene="xseA"
                     /locus_tag="VFH83_01080"
     CDS             2577..>3135
                     /gene="xseA"
                     /locus_tag="VFH83_01080"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET6484978.1"
                     /translation="MAVEPLRERVYSVSEITQLIKYTLEDGFPWVTIQGEISNFRPSS
                     TGHWYFSLKDAEAVLSIVMFRGRIEGVRFAPADGMLVTLSGSVSVYPRRGTYQLIAEV
                     MARSGVGDILAMLEERKRRLAAEGLFDEGRKRRLPLHPSRVAVVTSPTGAAIRDILRV
                     LGQRDAGVDVVIVPTPVQGDGADERI"
BASE COUNT          599 a          967 c         1058 g          511 t
ORIGIN      
        1 cctggaccag gataccggtt gccactcagc cgttctccca tcgctcgacg agctgtcggg
       61 gatccttgcg gaaccggaac cggtgggtcc atacccagga acggacggac cggaactcct
      121 tgatggcgta gaacggcccc gtatccgact tgatcatgta atcgaggagt cttcgacggg
      181 gcagcgttcc catctcccgt cggagacgga ccgcgacgca tttgaggaag aagagcttgt
      241 agccctggtc cggggtggca tcgtcggtgg gcgggctgcc ggtgtaggtg accgtgagct
      301 gaagggtccc acacaggcgc tctccccaca ggagacaccg aagcccgaaa tccagccgct
      361 gccagtaagg gttctggatg gcgggatcgt agcctccgct ctgggcgaac ctctgtcgat
      421 cgtagagacc acaatagtca aacgggtaga gcgtgggctc gccctctttt cggggagctc
      481 gaaaggtcac caggaaccga cgccgcctcc agcccggaga ttgccaggag gggatgatct
      541 ccagccgcgg gttgcgggaa accggaacca cgcatacagc gtgatgagac tccagatccc
      601 cgaggatggc tggccggaag gaggacaggc gggtatcgct ccaaagacag aggacctgag
      661 gagcgcgcgc ctcctcgatg cccacgttca cccactcccc ggcggtagcc cgcgcgccca
      721 ggagcagaaa tcggacctgg gggaaatcac gcgccaggct ctccacgtcc gaaggctggt
      781 ccggtccctc cacccagagg atctccccca ggtcctgctc gcaaagctcc ttgagcagct
      841 ccgcccggaa tagccgatcc cccctgctca gcacgagaat ggtcaggcga tacgggtagc
      901 gtcggacccg atcccatcgg gctccgccca gcacggtata ggggatgagt ggacgcggct
      961 cagaagttgt aggtgtagta ttcatcgcac cctgcgcaga tgccggggta ctgccgcgcg
     1021 atgtgggacc ggtacaccga ctgcccgcgc tcccagatga cggacagctc ctccgtgaag
     1081 gcgttgccca gcggcgtggt cactcggacg tcctcccgac acaagggaac cgtgccgtcc
     1141 aggaggatcg agagatcccg cttcaggtgc cagcagggga accgctggag gggagacagg
     1201 tctgccactc tacgatccgc aagcaacccg ccaaccggat cgtatttctg gacaatgacg
     1261 ttgtccgtgc gagctttcca ggacttgtag aagacgtcca ggtcgtcctc gttctctttc
     1321 atccggacgg cttgcaccca cgcggacctc gggaagaggg tcaggagggt ctcagcggac
     1381 cgcaccgcct ccgcgaagcc cttgccgcga agctgggtgt acaggtcctc tctcgtcgcg
     1441 tccagggaga caatccaggt gggctgcctc tggaagccat ctttgatccg tccgaagagc
     1501 gaaggatccc aaccaacccc ggaggtctct atcacgagcc gcaaggaggg ggtctcaagc
     1561 acggtgctga tgagatcgta tatggaaggg tggagagccg gttcccccca gagggagatc
     1621 ccgatcacgg catcgccaga gaaagcggaa atccgggccg caaggtcgga aaaccgttcc
     1681 gtcggcatca cgccggtctt gcgcatgacg tcaccggcaa cgaccggata ggggcagtag
     1741 gagcaggcgt gcgggcaacg ctccaccacc tggatggtga agtacgcggg cagtgtcctc
     1801 aggatctcgg ggttgtgctg gagggcgtcg cacaccgaag cggcatcccg ggctccggcc
     1861 gccacgacgc gctgaagctg gaggaagttg cgctgggagt ccgcggcaag gctcacgcgc
     1921 agaagccgct ggtccacggg agagagctcg gtttcgatgt cgtacgcgtt gatgtctttg
     1981 gagatgagag agaacagggt gtcccgttcc ggcacggcga cggaagccgc tgccttgagc
     2041 cggctcgcgg tttcccgagt caggatctcc ggggaaagcc cgtacggata gccatccgca
     2101 taggtgtagt ccgcccagta ccggcgatgg ttctcgtgca tgcgccgggc aagctcgata
     2161 tccaggaagg ggcagtcggc atagaagtag aacacgtcct cgaagccggc cgcggcatcg
     2221 accatctgcg ccaggagctc cgcctgggtc caggagtctc ggatcacggt cctcatcccg
     2281 gccgcggcct cgaggggccg ggagagaaac atcacccagt cctggacacc ggggagggat
     2341 cgggcaaagg ccgtcacgcg gtccaccgcg ctggcgccat ctatgagcgg caggagggca
     2401 tgcctgccca gagagatggc gtttatgacg gcagtgttct tcatgactcc cggtgatagt
     2461 atcgacagtt tccgaggaag ttcaaggaac gcggcgcgcc ggaatcctgc gcaggtggcc
     2521 ccgaagcggc agctacgacg ggttgccacg ccctacggct gcgggtaggc tgaggtatgg
     2581 cggtggaacc cctccgggag agggtctact cggtatcgga gatcacgcag ctcatcaagt
     2641 acaccctgga ggacgggttc ccctgggtga cgatccaggg agagatctcc aatttccggc
     2701 catccagcac cgggcactgg tatttcagcc tcaaggacgc ggaggcggtc ctctccatcg
     2761 tcatgttccg gggaaggatc gagggggttc gcttcgcgcc cgcggacggg atgctggtca
     2821 ccctgtccgg cagcgtctcc gtgtatccac ggcggggaac ttaccagctc atcgcggagg
     2881 tcatggcacg gtccggtgtc ggggacatcc tggccatgct ggaggaacgc aagcggcggc
     2941 tcgcggcgga gggactcttc gacgagggga ggaaacggag gcttccgctc cacccctccc
     3001 gggtagcggt ggtcacctca cccacgggcg ccgccatccg ggacatcctc cgcgtcctgg
     3061 gccagcggga tgccggggtg gacgtcgtga tcgttcccac gccggtccag ggggacggcg
     3121 cggatgagcg gatcg
//
LOCUS       DASRZS010000046         5065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1565659, whole genome shotgun sequence.
ACCESSION   DASRZS010000046 DASRZS010000000
VERSION     DASRZS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565659"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFH83_01085"
     CDS             <1..445
                     /locus_tag="VFH83_01085"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET6484979.1"
                     /translation="LMAPFLSEAGLPVTDTAMLEGIASLVRERIKRLTDVPAMVQFLF
                     VDPPKPSLEELLPKKTDAQKALAALSRLQTILPELLGPEEDNEAKLKGLAAELGMKVG
                     DMLMPLRVAVTGASVSPPLFQSIRLLGVEKARARTARAISVLAGA"
     gene            497..1867
                     /locus_tag="VFH83_01090"
     CDS             497..1867
                     /locus_tag="VFH83_01090"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HET6484980.1"
                     /translation="MSEEAKPQAAGAEALTMDKLVSLTKRRGFIFPSSEIYGGLASAW
                     DYGPMGVELKKRIQEFWWRGMTQLNDNIVGLDAAIMMHPRVWEASGHVENFTDPLIDC
                     KQCKHRFRADQIPEENLKERKCPDCGAKALTDARKFNLMFTTHMGPVEDEGGLIYLRP
                     ETAQGIYVNFKNVLQTGRVKIPFGIAQVGKAFRNEITTKNFIFRTCEFEQMEMQYFIH
                     PSEDAKWFEHWKEKRFQYYQDLGIRASKLRFHQHGPDELAHYAKLAFDIQYEFPFGWR
                     ELEGIHNRTDYDLKRHAEFSGKDLTYLDEQSKERFVPYIIETSAGLTRTVLVALCDAY
                     EEEVVEGETRVVLRLHPRIAPITVGVFPLVRKDGLAEMGRNLDTELCEEFRTFYDESG
                     AIGRRYRRQDEVGTPFCVTLDYQTKEDQTVTIRFRDSMQQVRVKMAEIAPTIRKAIKE
                     YRRPGA"
     gene            1864..3150
                     /gene="tyrS"
                     /locus_tag="VFH83_01095"
     CDS             1864..3150
                     /gene="tyrS"
                     /locus_tag="VFH83_01095"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET6484981.1"
                     /translation="MSAASTVSDTVQAGAFDVLKERGFVSQCSDEAGLRRLLDTEQVT
                     YYIGFDPTAPSLQMGNLAQIMLMAHLQRAGHRPIALVGGGTTRIGDPSGKTEMRKILP
                     VETIAANAEVFKRQISRFLDFAPGKALMLDNAEWLAPLNYIEFLRDIGRHFSVNRMLT
                     FETYKMRLETGLSFIEFNYQLLQSYDYLVLCRRHGCRLQMGGDDQWGNIVAGIDLIRR
                     VEGVEAYALTSPLVTRSDGQKMGKSEKGALYLDPAMTSPYEFYQYWLNVPDADVRQFF
                     LRFTFLPVEEVTEICSVEGERINDAKRRLAAELTTLVHGAPAAKEAADASRAAFGGGS
                     AGGADGEPGDLSAVPSLEVPRSDLAGGISVVDLFVRSGLCSTKSDARRLVTQGGAYVS
                     RRNITDIDARVTTEAAENGVILLRAGKKRYFRIVLA"
     gene            3187..3729
                     /locus_tag="VFH83_01100"
     CDS             3187..3729
                     /locus_tag="VFH83_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484982.1"
                     /translation="MKRLGKTRFARYLAAMARTVHPIRAIFEVCGTILFAIPFLAGFA
                     VNRSAPLYLYLLYPLLFLVSHFVWFRLPSVDRRVRWILFWVTLTLVAALGAGFIYWSY
                     LEAVARGARLHPLGIFAIPHWVSRRIDVPFPTVALYFLAPSAAGAAVYCAVLALFLRI
                     YRRYRVEEHHRRTGSDGTGS"
     gene            3726..>5065
                     /locus_tag="VFH83_01105"
     CDS             3726..>5065
                     /locus_tag="VFH83_01105"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484983.1"
                     /translation="MSARNAGEARLTVTRLLAAAVVAASLVLGFLHRPAPLTIGDHLY
                     YLFLAANAGFAIALFAREGGEVPSLKRSYLFYRPPEILLALGVVLFLFAFIYATNANM
                     SYVQRFMATHGNIHLAPDSTRERLAACIQYLPVLALDAFWYVYYRVRYPALRRGTLAS
                     SRRAGTWALGAAILSAALYAFAFPSILRTRGLPFLSYIALVPLLVSLEECSLGWGVFY
                     GTCFGVLQTMIGSYWLGTFDLLSLQFVTVVTLLEYIPFLVVSLLALRWSRGLGFLVFP
                     AAWTAFDWLRSQGFLGYPWGMPGASQYSVIPLIQIASFTGVWGVTFLVVLCNSVVAWY
                     LGGGRRFTRFPAPAIVLAAAAVLTLGWAGYGYLRARAAGSAPASVRLALIEQDSDPRK
                     DNYDDTFRTLQDLTNRALAERPDLVVWSETAFVPNIRRWSAEDPKVYPLAALVR"
BASE COUNT          810 a         1706 c         1651 g          898 t
ORIGIN      
        1 cctgatggct ccgttcctgt ccgaagccgg cctccccgtg accgacacgg cgatgctgga
       61 gggaatcgct tccctggttc gtgagaggat caagcgcctc acggatgtgc cggccatggt
      121 ccagttcctc ttcgtggatc cgccgaagcc cagcctcgag gagctgcttc ccaagaagac
      181 ggacgcccag aaggcgctgg ccgccctgag ccggctccag accatcctgc cggagctcct
      241 gggtcccgaa gaggacaacg aggcgaagct caagggtctg gccgccgagc ttggcatgaa
      301 ggtcggtgac atgctcatgc ccctgcgggt ggcggtgacc ggcgccagcg tctccccgcc
      361 gctctttcag agcatccggc tcctgggcgt ggagaaggcg cgcgcccgaa cggcccgggc
      421 catctcggtc ctggcgggag cctgacgcgg cgcgggtcgc ggctgcacgg ccacggacgt
      481 ggccaaggag atgacaatgt cggaagaggc gaaaccccaa gcggcgggtg ccgaagccct
      541 cacgatggac aagctcgtgt cccttaccaa gcgccggggg ttcatcttcc cgtccagcga
      601 gatctatggc ggcctggcct cagcgtggga ctacggtccc atgggggtcg agctgaagaa
      661 gcgcatccag gagttctggt ggcgggggat gacccagctc aacgacaaca tcgttggcct
      721 ggatgccgcc atcatgatgc acccgcgcgt ctgggaagcc tccggccacg tcgagaactt
      781 cacggacccg ctgatcgact gcaagcagtg caagcaccgc ttccgcgcgg accagatccc
      841 ggaggagaac ctcaaggagc ggaagtgccc ggactgcggc gccaaggcgc tcaccgacgc
      901 ccgcaagttc aacctcatgt tcaccaccca catgggaccc gtggaggacg agggcgggct
      961 gatctacctg cggcccgaga cggcccaggg catctatgtg aacttcaaga acgtgctcca
     1021 gaccgggcgg gtgaagatcc ccttcgggat cgcgcaggtg ggcaaggcgt tccgaaacga
     1081 gatcaccacc aagaacttca ttttccggac ctgcgagttc gagcagatgg agatgcagta
     1141 cttcatccac ccgtcggagg acgcgaagtg gttcgagcac tggaaggaga agcgcttcca
     1201 gtattaccag gacctgggga tccgcgccag caagctgcgc ttccaccagc acgggccgga
     1261 cgagctggcg cactatgcca agctcgcctt cgacatccag tacgagttcc cgttcggctg
     1321 gagggagctg gaagggatcc acaaccgcac cgactacgac ctgaagcgcc acgcggagtt
     1381 ctccggcaag gacctgacgt acctggacga gcagagcaag gagcggttcg tcccgtacat
     1441 catcgagacc tcggccggtc tcacgcgaac ggtgctggtt gccctttgcg acgcctatga
     1501 ggaggaggtg gtggaagggg agacccgggt ggtcctccgc ctccatccaa ggatcgcgcc
     1561 catcaccgtg ggcgtattcc cgctggtgcg caaggacggc cttgccgaga tgggccgcaa
     1621 tctcgacacc gagctgtgcg aggagttccg caccttctac gacgagagcg gggccatcgg
     1681 ccgaaggtat cgccggcagg acgaggtggg cacgccgttc tgcgtgactc tggactacca
     1741 gaccaaagag gaccagaccg tcaccatccg gttccgcgat tccatgcagc aggtgcgggt
     1801 gaagatggcg gagatcgccc ccaccatccg caaggccatc aaggagtacc ggaggccggg
     1861 agcgtgagcg cagcgtccac cgtctccgat accgtccagg ccggcgcgtt cgacgtcctc
     1921 aaggagcgcg ggttcgtgag ccagtgctcg gacgaggcgg ggcttcgaag gctcctggat
     1981 accgagcagg tcacctacta catcgggttc gatccaacgg cgcccagtct ccagatgggc
     2041 aacctggccc agatcatgct gatggcccac ctgcagcgcg ccggccaccg gcccatcgcc
     2101 ctggtgggag gcggaacgac acggatcggg gacccttcgg ggaagaccga gatgcggaag
     2161 atccttcccg ttgagaccat cgcggccaac gcggaggtct tcaagaggca gatctcccgg
     2221 ttcctggatt tcgcgcccgg caaggccctg atgctggaca acgccgagtg gctggcgccg
     2281 ctgaactaca tcgagttcct tcgcgacatc ggccgccact tctcggtgaa ccggatgctc
     2341 acctttgaaa cgtacaagat gcggctggaa accggactgt cgttcatcga gttcaactac
     2401 cagctcctcc agagctacga ctacctggtg ctctgccgcc gtcacggatg ccgcctgcag
     2461 atgggcgggg acgatcagtg gggcaacatc gtggccggca tcgatctgat ccgccgcgtg
     2521 gagggcgtgg aggcttacgc gctcacctct ccgctggtga cgcgctcgga cggccagaag
     2581 atgggcaagt cggagaaggg cgcgctgtac ctggatccgg ccatgaccag cccgtacgag
     2641 ttctaccagt actggttgaa cgtgccggac gcggacgtgc gccagttctt cctgcgcttc
     2701 acgttcctcc ccgtggagga ggtgacggag atatgctccg tagagggcga gcggatcaac
     2761 gacgccaagc gccggcttgc cgcggagctc accacgctgg tccacggcgc tccagcggcc
     2821 aaggaggcgg cggacgcctc ccgggccgcg ttcggtggcg gctcggcggg cggcgcggac
     2881 ggggagccgg gcgacctctc cgcggtgccg tcgctggagg tcccacgctc cgatcttgcc
     2941 ggcgggatca gcgtggtgga cctgttcgtc cgttcggggc tctgctcgac caagagcgac
     3001 gcccgccggc tggtcactca gggcggagcc tacgtcagca ggcgcaacat cacggacatc
     3061 gatgcgcggg tgaccacgga ggcagccgag aatggcgtca tcctgcttcg ggccggcaag
     3121 aagcggtact tccggatcgt gctggcctga cgggcccgcc gcggcgcccg gggcacgggg
     3181 gccggcgtga aacggctggg caagacccgg ttcgcgcggt atctcgcggc catggctcgg
     3241 accgtgcacc cgatccgtgc catcttcgaa gtgtgcggga cgatcctgtt cgccatcccg
     3301 ttcctggccg gctttgccgt caatcgttcg gcgcccctgt acctctacct tctctatcct
     3361 ctcctgtttc tcgtgtccca cttcgtctgg ttccgcctgc cttccgtgga ccgaagggtc
     3421 cgctggatcc tcttctgggt cacgctcacc ctcgtcgcgg ccctgggcgc cggcttcatc
     3481 tactggagct acctggaagc ggtggcgcgc ggggcgcggc tccatcccct gggcatcttc
     3541 gccattcccc actgggtaag ccgccgcatc gatgtccctt tccccacggt ggcactgtac
     3601 ttcctcgcgc cttccgcggc cggcgccgcc gtctactgcg ccgtgctggc gctcttcctg
     3661 aggatctacc ggcgctaccg cgttgaagag caccaccgcc ggaccggttc ggacggcacc
     3721 gggtcatgag cgctcggaac gccggcgagg cccgtctgac ggtgacccgg ctcctcgccg
     3781 cggccgtggt cgcggcttcc cttgtgcttg gattcctgca tcgccccgcg cccctgacca
     3841 tcggcgacca tctctactac ctgttcctgg ccgcaaatgc ggggttcgcc atcgccctgt
     3901 tcgcgcggga gggaggagag gttccgtccc tgaagcgctc ctatctgttc taccggccgc
     3961 ccgagatcct gttggccctg ggggtggtgc tcttcctgtt tgccttcatc tatgcgacca
     4021 acgcgaacat gagctacgtc cagcggttca tggcgactca cgggaacatc cacctggccc
     4081 cggatagcac gcgcgagcgg ctggccgcgt gcatccagta tctccccgtg ctcgcgctgg
     4141 atgccttttg gtacgtctac taccgggtgc gctaccccgc gctgcgacgc ggcacgctgg
     4201 cgtccagccg acgcgccggt acctgggccc tcggggcggc catcctctcc gccgccctgt
     4261 atgccttcgc cttcccatcg atcctgcgga cgcgtggcct cccgttcctc tcctacatcg
     4321 cgctggtccc gctgctcgtc tccctggagg agtgcagcct ggggtggggc gtgttctacg
     4381 gcacgtgttt cggcgtcctc cagacgatga tcggcagcta ctggctgggc acgttcgatc
     4441 tgctgagcct ccagttcgtc accgtcgtca cgctcttgga gtacattccc tttctggtgg
     4501 tatccctcct tgccctccgg tggagccgag ggctgggctt cctggtgttc ccagcggcgt
     4561 ggaccgcctt cgactggctg cggtcccagg gattcctcgg ctacccgtgg ggaatgccgg
     4621 gcgcgtcgca gtacagcgtg ataccgctga ttcagatcgc atcctttacc ggagtctggg
     4681 gcgtcacctt cctcgtggtc ctgtgcaact cggtggtggc ctggtacctc gggggcggca
     4741 ggcgattcac ccgctttccc gccccggcaa tcgtccttgc cgcggcagcc gttctgacgc
     4801 tcggctgggc aggctatggg tacctccgtg cccgcgccgc gggaagcgct cccgcgagtg
     4861 tccgtctggc gctgatcgag caggattccg atccccgcaa ggacaattac gacgacacct
     4921 tccgcacgct gcaggacctt accaaccggg ccctcgccga gcggccggac ctggtggtct
     4981 ggtccgagac cgccttcgtg cccaacatac gacggtggag cgccgaggat cccaaggtgt
     5041 acccgctggc ggccctggta cggag
//
LOCUS       DASRZS010000047         5179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4262306, whole genome shotgun sequence.
ACCESSION   DASRZS010000047 DASRZS010000000
VERSION     DASRZS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4262306"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="VFH83_01110"
     CDS             complement(<1..1254)
                     /locus_tag="VFH83_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6484984.1"
                     /translation="MTLRSLLLSGALLMAAATLEADRLTLDALPALVLGHDPQAAISE
                     QTLKVALHTYQGNLSQTLPQIDLSTTYSLGFTPSQESQSTTFTTLFPYLQVADETFTN
                     MGNHALNAKLSVSQILPTAGSLFLSLENTTSASTISSQTIAGAQSNPDNQFAERPKLT
                     IGVTQPIFLNGKVLDLDLFPATIRKAELGYKEQDLATRAQRNLSVAQAVQTYLLVVQL
                     RANVAQTTKSIAVTQGTVAAAEKNYSLGLLAEADLLDARIGLSRQQQGLLELSSSLGR
                     AERALAHLIGRDDLQGMDLSDTVPVVALSVDQDRILDRALANHPLLVQKGMVAEERRL
                     DEVLGGQASASTLSLSFSYAPRYPFSTTVPYMTTFPNSFSDLFASGSGQDYSLSAGLT
                     IHLFDGGKAAQNKAAAQAVSAAAAQS"
     gene            complement(1257..2051)
                     /locus_tag="VFH83_01115"
     CDS             complement(1257..2051)
                     /locus_tag="VFH83_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484985.1"
                     /translation="MIRGVFAVPGALIRPDRAFAGRDVYQLSTTILILALFLCHIVGG
                     RLVQGYFQNAHAKMLAIAEADARMSGLLANAPPEVQTRARTQMLSSVLGRGSSVITSF
                     GIVVSGVEFVGLCAELWLVSLVAAQFFGGQEERHGRPRPSWILFLVAFVPLALRSLLT
                     GVLMTLGNASAQNAITLEDYRRLSIPSLDLYSILHGGLPWPFAAALARFLTDPFFLWS
                     FAILVAGGRAVYRIPLRGAIWLVVVLVIVLSLQTALLARIGVTWEI"
     gene            complement(2151..>5179)
                     /locus_tag="VFH83_01120"
     CDS             complement(2151..>5179)
                     /locus_tag="VFH83_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6484986.1"
                     /translation="LRNYKQLVRLRSASLPEVESAITAVLGGGATSASPGVWTVRLGE
                     EKECSPTAVAQLAWRLREFLSLRRKDLFGSAVLIASIGEERRKEEIIRGLLETGDRVD
                     QLWITPECAGLLSKVLVFEPSGDLFRVIEPRRAPPVVTAVRVSEDRSWAREALIERAL
                     DLLTPRLNDSEARDVVLVHGPPGVGKSEFLRQLSFRLLRKTDTAPIRAYTVFRRRSIL
                     HPFLNSLSPQLLAATPLALTPLERAVWEETGELLKWLRGPSVWAAGPLPDRIEEDFAL
                     AYRLYLLSWVRTAEAKGAPAIFLCEGIESYHPAARRLLARLVEELMASSVGFLPLLSS
                     TAAEPPEELARLNVRPLYLHPLGKREIRALSGHLFSGLDLPEGLARRLRRRSGGRYLT
                     VISYLHYLRKTGSIRAVGDRFQWAPASEEALPDHPQAVSWYLIRSRPNDLYLLLYVLY
                     LAAGLLDRSGLIIFLAEAGFDLSTVQRYLDDLAAFGLVAEPGSPIPRFPALRRNLEGL
                     LGTEGAELRERFISHLWSLWRDGRYNHPVLLFTFFARNGENDRALDVLAEIVRRKLDE
                     NDVAGAAAFCEADRMEFAAPLSTEQQRVLASITITGRLRSALLQNDVAAAHAAQADAE
                     ASDTAPDGPAADDLRIEQAKFLVSRGQIGPAIDELKRSLLGSQDARDQRPRDAAAARG
                     ERASYLWLGSAMLADGRSGEAVEYLRMSTRLSEEARDASAELWSGVTLGATLFIDGRF
                     TQCLAMLEQAVRRARLAYRHQVELYLVFLQGRSHFELGEYEDASQRFQSGLSLATLYH
                     AESALPVFRAWLGRTQLHIGEWEAGAGLLSSLDRTGEVLLFLAEGALLSRDLDGAWNW
                     AEQSLMAADPPLLFSPEGITWWDGFSLVEGKCQRLTRGGAYLRRLIVGVHAYLQGLRG
                     DRANAYQDFRQMTRGERPVEDDPASYLLNFLYSRILPEAGSEEIDDKLTVLGKSLKSL
                     QVRSSRIDAPAQRSSFLWRNHWNRLIMEEARERKLA"
BASE COUNT          903 a         1677 c         1833 g          766 t
ORIGIN      
        1 gctctgggcc gccgcggcgc tcaccgcctg agccgccgcc ttgttctgcg ccgccttgcc
       61 gccgtcgaag aggtggatgg tcaggccggc gctcaggctg tagtcctgtc ccgagccgct
      121 ggcgaacagg tcggagaacg agttgggaaa cgtcgtcatg taggggaccg tggtcgagaa
      181 cggatagcgc ggggcatagg aaaacgaaag gctgagcgta gacgcggagg cctggccgcc
      241 caggacctca tccagccggc gctcttccgc caccatcccc ttctggacca ggaggggatg
      301 gttggccagc gcgcggtcca ggatccgatc ctggtccacc gacagcgcca cgacagggac
      361 cgtgtcactc aaatccatgc cctgaagatc gtcgcggccg atgaggtggg ccagggcccg
      421 ctcagccctt ccgaggctgg aggaaagctc caggagcccc tgctgctgac gcgagagccc
      481 gatgcgggcg tcgagcagat ccgcttccgc caggagaccg agggagtagt tcttctcggc
      541 ggccgccacg gtgccctgcg tcacggcgat gctcttggtg gtctgggcca cgttggcccg
      601 aagctgaacc accaggagat acgtctggac ggcctgagcc acggacaggt tgcgctgggc
      661 gcgggtggcc aggtcctgct ccttgtagcc caactcggcc ttgcggatgg tggcgggaaa
      721 cagatccagg tcgaggacct tgccgttcag gaagatgggc tgggtgaccc cgatggtcag
      781 cttgggcctc tccgcgaact ggttgtccgg attgctctga gcgccggcaa tcgtctgact
      841 ggagatcgtg ctggcgctcg tcgtgttctc cagggagagg aagaggctgc cggcggtcgg
      901 cagaatctgg gacaccgaga gcttggcgtt cagtgcgtga ttgcccatgt tcgtgaaggt
      961 ctcatccgcc acctggaggt aggggaagag cgtggtgaac gtggtggact ggctctcctg
     1021 gctcggggtg aaccccaggg agtaggtcgt ggaaaggtcg atctggggca gcgtctggga
     1081 gaggttcccc tggtaggtgt gcagggccac cttgagggtc tgctcggaga tggcggcctg
     1141 gggatcgtgc cccagcacca gcgccgggag ggcatccagg gtcagccgat cggcttccag
     1201 agtcgcggcg gccatgagga gcgcgccgct cagcaacagg gaacgtaacg tcatggtcag
     1261 atctcccagg tgacgccgat ccgcgcgagg agggcggtct gaaggctcaa cacgattacg
     1321 agcaccacca ccaaccagat ggctccccgc agcgggatgc ggtagaccgc gcggcctcca
     1381 gccaccagga tcgcgaagct ccagagaaag aacggatcgg tcaggaacct cgccagtgcg
     1441 gcggcaaacg gccacggcag accgccgtgc aggatcgagt agaggtccag gctgggtatg
     1501 ctgagacgcc gatagtcctc gagcgtgatc gcgttctgcg cggaggcgtt ccccaaggtc
     1561 atgaggacgc cggtgagcag gcttctcagg gccagcggga cgaaggcaac caggaacagg
     1621 atccaggatg gccggggccg accgtgtcgt tcctcctggc cgccgaagaa ctgggcggcc
     1681 accaggctca ccagccaaag ctccgcgcag aggcccacga actccacgcc ggacaccacg
     1741 atgccgaagg aggtgatgac gctgctgccc ctacccagga ccgaggaaag catctgggtc
     1801 cgggcccgcg tctggacctc cggcggcgcg ttggccagga ggccgctcat gcgggcgtcc
     1861 gcctccgcta tggccagcat cttggcgtgg gcattctgga agtacccctg gaccagtctg
     1921 cccccgacga tgtggcacag gaagagggcc aggatcagga tcgtggtgga cagttggtat
     1981 acatcgcgcc ccgcgaaggc ccggtcgggc cggatcaggg cgccaggcac ggcgaaaact
     2041 ccccggatca tgagcttttc gagagcttcc gcgattaagg gatgtgagtc aagggatctg
     2101 ggaaccgggc ggccgacgcc cgccctgggc ctccgctcag aatggccggc tcaggcgagc
     2161 ttgcgctccc gggcttcctc catgatcagg cggttccagt ggttgcgcca gaggaaggag
     2221 ctccgctgcg ccggggcatc gatccggctg gaacgcacct ggaggctctt gagcgatttg
     2281 cccaggacgg tgagcttgtc gtcgatctct tcggaccccg cctccggtag gatacgggag
     2341 tagaggaagt tcaggaggta ggaggcggga tcgtcctcca cgggcctctc accgcgggtc
     2401 atctgccgga agtcctggta ggcgttggcc cggtctcccc gaagaccctg gaggtaggcg
     2461 tgcacgccga cgatcagtcg ccgaaggtac gcgccgcccc gggtcagtct ctggcatttc
     2521 ccttctacaa gggagaaccc gtcccaccag gtgatgccct ccggcgaaaa gagcaggggc
     2581 ggatccgcgg ccatgaggct ctgctccgcc cagttccacg cgccatccag gtcccgcgac
     2641 aggagcgccc cttccgccag gaagagcagc acttccccgg tccgatcgag gctcgagaga
     2701 agaccggccc ccgcctccca ttcgccgatg tggagctggg tgcgtcccag ccatgcgcgg
     2761 aatacgggaa gcgcgctctc cgcgtggtac agggtggcaa gggagaggcc gctctggaaa
     2821 cgttgggagg cgtcttcgta ctccccgagc tcaaagtggg agcggccctg gagaaacacg
     2881 aggtagagct cgacctggtg acggtacgcc agccgcgccc ggcgcacggc ctgctccagc
     2941 atggccaggc actgggtgaa ccgcccgtcg atgaagagcg tggcgccgag cgtgacgccg
     3001 ctccacagct ccgccgaggc gtcgcgggcc tcctcggaga gccgcgtgga catccgcagg
     3061 tactccacgg cttccccgga gcgcccgtcc gcgagcatgg ccgagcccaa ccagagatag
     3121 gaggcgcgct cgccccgggc ggccgcggcg tccctaggcc gttggtcccg ggcgtcctgg
     3181 ctgccgagaa gagatcgctt cagctcgtcg atggctggcc cgatctgccc gcgggagacc
     3241 aggaacttgg cctgctcgat ccgaaggtca tcagccgccg ggccgtcggg ggccgtgtcg
     3301 gatgcctcgg cgtccgcctg ggccgcgtgg gcggcggcca cgtcgttctg aagcagcgcg
     3361 gagcgaagcc tgcccgtgat ggtgatcgag gcgagcaccc gctgttgctc cgtggaaagc
     3421 ggagccgcaa actccatgcg gtccgcctcg cagaaggcgg cggcgccggc cacgtcgttc
     3481 tcgtcgagct ttctccgcac gatctccgcc aggacgtcca gcgcacggtc gttctccccg
     3541 ttgcgggcga agaatgtgaa gagcagcacg gggtggttgt atcgcccgtc cctccacaaa
     3601 gaccacaggt gagagatgaa gcgctcccgc agctccgctc cctccgtgcc caggaggccc
     3661 tcgagattcc gccgcagcgc cgggaaccgc ggtatgggcg atcccggctc cgccaccagg
     3721 ccgaacgcgg ccagatcgtc caggtagcgc tggaccgtgg agagatcgaa gcccgcttcc
     3781 gccaggaaga tgatgagacc cgagcggtcc aggaggccgg ccgccagata cagcacgtac
     3841 agcaggaggt acaggtcgtt cggccgggag cggatgaggt accacgacac ggcctgcggg
     3901 tggtccggca gcgcctcctc cgaggcggga gcccactgga atcggtcccc gacggcgcgg
     3961 atgctgcccg tcttccgcag gtagtggaga taggagatca cggtgaggta gcgcccgccc
     4021 gacctgcggc ggagccgccg cgccagtcct tccggcagat ccaggccgct gaagaggtgt
     4081 cccgaaagcg cgcggatctc gcgcttgccc agcggatgca ggtacagggg ccgcacgttg
     4141 agccgggcca gctcctccgg aggctcggcg gccgtggaag agagcagagg gagaaatccc
     4201 acgctggagg ccatcagctc ctcgacgagc cgggccagga gccgccgggc cgcgggatgg
     4261 taggactcga tgccttcaca gaggaagatg gccggcgcgc cctttgcctc cgcggtccgc
     4321 acccaggaga gaaggtacag gcgatacgcg agggcgaaat cctcctcgat acggtccggc
     4381 agcgggccgg ctgcccagac tgaggggccg cgcagccact tcaggagctc gccggtctcc
     4441 tcccagaccg cgcgctccag cggggtgagg gcgagcgggg tggccgccag gagctggggc
     4501 gagaggctgt tcaggaacgg gtgaagaatc gatcgacgcc gaaaaacggt gtatgcgcgg
     4561 atgggggccg tgtcggtctt gcgcaggagg cggaagctga gctggcggag gaactccgac
     4621 ttcccgacgc cgggggggcc gtgcaccagc accacgtccc gcgcctcgga atcgttcagc
     4681 cggggcgtga gcagatccag ggcgcgctcg atgagcgcct cccgggccca ggagcggtcc
     4741 tcactgacac ggaccgcggt gacgacgggc ggcgctcgac gcggctcgat cacccgaaag
     4801 agatccccgg agggctcgaa gaccaggacc ttcgacaaca ggccggcgca ctccggagtg
     4861 atccagagct gatccacccg atctcccgtc tccagaagac ctcggatgat ctcctctttg
     4921 cggcgctcct ccccgatgga ggcgatcagc accgcagacc cgaagaggtc cttgcggcgg
     4981 agggacagga actcccgaag ccgccaagca agctgggcca cggccgttgg ggagcattcc
     5041 ttctcctccc ccaggcgcac ggtccagacc ccagggctgg ccgatgtggc gcctcctccc
     5101 agcacggcgg tgatggccga ctccacctcc ggcaagctcg ccgagcgcag ccgcacgagt
     5161 tgtttgtagt tccgcagct
//
LOCUS       DASRZS010000048         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3877725, whole genome shotgun sequence.
ACCESSION   DASRZS010000048 DASRZS010000000
VERSION     DASRZS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3877725"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VFH83_01125"
     CDS             <1..725
                     /locus_tag="VFH83_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6484987.1"
                     /translation="RHLEAAAEAHDALVVFKQATARSVVDSLFSFYQRDIRNAVLWPY
                     DEPVEIEKLQRLRGLGMNLVLFDRTIGVPWADCVTVDNGHAVRTLRAHLAAQGARRIG
                     YLGWENDVLTSNLERETAFVEMEGPDRLVRIPWTKETGLERLIPSLVRSMIDRADALL
                     CGNGVIGIEVGRYVRGAGLSQRVACVDDLPGASDIGLTAYSQPMDRLSNTAYRLLAAQ
                     SASAGGWKAGIYRLEGSLVVRA"
     gene            760..>2057
                     /locus_tag="VFH83_01130"
     CDS             760..>2057
                     /locus_tag="VFH83_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET6484988.1"
                     /translation="MAERRDRILTVDHRYLVFPRWRDAGNPTSDAVSVAIDGEPYFAV
                     GGAGLASHEPDFYTFLDLDHARGREVTVEIEGENADAIDLVRTADRIPSAYVPYGEPE
                     RPLVHFSPLRGWLNDPSGLLYLDGQWHLYYANNRFANRMAGPDNAWAHAVSPDLVHWR
                     EVPLFLCPIRGTRSFWTGGAAIDIANTTGEGRPGSPVVVFSANNGSDAPNAFTQCVFV
                     STDGGATCTLDPRRMYVPLPAEAHRRGGGTRDPMILWWEPERKWVMIVYNHPPGGRPG
                     FYFFESRDLTSWAETSVLEDQFECPNLVVLPVDGNPSDSRWVIWGSSSEYLVGSFDGS
                     RFHPDGQGKLRTHHGPFTASQVFANAPVGRVVQVGWAHCCNFDREFSQMASFPLELSL
                     RSTGAGVRLHAEFVEEIRSLRLDGWQWSDVAIEVGALLIG"
BASE COUNT          344 a          661 c          678 g          374 t
ORIGIN      
        1 tccgacacct ggaagccgcg gcggaggcac acgacgcgct ggtggtcttc aaacaggcaa
       61 ccgcgcgctc cgtcgtcgat tcgctcttca gcttctacca gcgggacatc cgcaacgcgg
      121 tgctctggcc gtacgacgag ccggtggaga tcgagaagct gcagcgactg cgcggactcg
      181 gcatgaacct ggtgctgttc gaccggacca tcggggttcc ctgggccgat tgcgtcaccg
      241 tggacaacgg ccacgcggtg cgaaccctcc gcgcccacct ggctgcccag ggggcgcgtc
      301 gtatcggata cctggggtgg gagaacgacg tcctcaccag caacctcgaa cgggaaaccg
      361 cgttcgtaga gatggaggga cccgaccgac tggttcggat tccctggacg aaggaaacgg
      421 gactggagag gctcatcccc tccctcgtcc gctcgatgat cgatcgcgcc gacgccctgc
      481 tctgcgggaa cggcgtcatt gggatcgaag tgggtcgcta cgttcggggc gcgggcctgt
      541 ctcagagagt cgcctgcgta gacgaccttc ccggcgcctc ggacatcggg ttgaccgcgt
      601 actcccagcc catggatcgg ctctccaaca ccgcctaccg attgctcgcg gcacagagcg
      661 cttccgccgg gggctggaag gccggcatct atcgattgga ggggagcctg gtcgtgcggg
      721 cctgagccgg gcttcgctca agcgggaacg ggggaatccg tggccgaacg aagggaccgc
      781 attctgaccg tggatcatcg gtacctggtc ttcccgcggt ggcgggacgc gggcaacccc
      841 acgtcagacg cggtatccgt tgccatcgat ggcgagccat actttgccgt tggcggcgca
      901 gggctcgcct cccacgagcc cgacttctac acgttcctcg atctggatca tgccagaggc
      961 cgtgaggtca ccgtcgagat cgaaggggag aacgcggacg ccatcgatct ggtgcgcacc
     1021 gcggatcgga tcccgtccgc ctatgttccc tatggcgaac cggagcggcc cctcgtccac
     1081 ttttctccgc ttcgaggctg gttgaacgat ccttccggcc tcctctatct cgatggccag
     1141 tggcacctct attacgccaa caaccggttc gccaaccgga tggcgggacc ggacaacgcc
     1201 tgggctcatg ccgtgagccc ggatctggtc cactggcgcg aagttcccct attcctgtgc
     1261 cccatccgtg gcacacgctc cttctggacc ggcggagctg ccatcgacat cgcgaatacc
     1321 acgggtgagg gccgtccggg gagtccagtc gtggtgttct cggcaaacaa cggaagcgac
     1381 gcacccaacg cgttcactca gtgtgtgttc gtgagcacgg acgggggagc gacctgcacc
     1441 ctggaccccc gaaggatgta tgtcccgctg ccggccgaag cccatcggcg gggcggcggg
     1501 acccgggacc cgatgatcct ctggtgggag cccgagcgga agtgggtgat gatcgtctac
     1561 aatcacccgc ccggaggcag acccggcttc tacttcttcg agtctcgaga cctcacgagc
     1621 tgggcagaga ccagcgtcct ggaggatcag ttcgaatgcc cgaacctcgt cgtcctgccc
     1681 gtggacggca atccctccga ttctcgctgg gtgatctggg gctcgagcag cgagtatctg
     1741 gtcggctcgt tcgacgggag ccggttccat ccggatggtc aaggcaagct gcggacccat
     1801 cacggtccat tcaccgcgtc ccaggtattc gccaatgctc cggtgggaag ggtggttcag
     1861 gtcggttggg cgcactgctg caacttcgat cgggagttca gccagatggc atccttccct
     1921 ctggagttga gcctgcggtc gaccggcgca ggcgttcgac tgcacgcgga gtttgtcgag
     1981 gagattcgtt ccctccgtct ggacggatgg cagtggagcg acgtggcgat cgaggtcggc
     2041 gccttgctga tcggcga
//
LOCUS       DASRZS010000049         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4649517, whole genome shotgun sequence.
ACCESSION   DASRZS010000049 DASRZS010000000
VERSION     DASRZS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4649517"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="VFH83_01135"
     CDS             complement(<1..1159)
                     /locus_tag="VFH83_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484989.1"
                     /translation="MPRHLPIRLLLAAMILCAVPHAVPLWPDAPPPTDAGTPQVVTSP
                     PEAPTTILSTQLGGADVRLDLLGSWTATASFGTGLLLLPGVAPQPLDAFPQLDQGFIF
                     NQTPDLTISLELLKRFFVDANVVGDLGSDAIHLGYQGAPGEVVQLVRVGTADITMDPT
                     PLLAVPNQATGSLGAQASFQSGASTNDVLLRWDVTRRKEKTFIGRNELVEQHVPLDSF
                     VRGRYFFLPDTGLDPNTLQVFIEDPQGTYRSGPTDGSRLYRLAGFDDVVLDTTQGLVS
                     LRNPVKGRVLVHYRKAGFEVGDPAIGVGGLPKDNGTVRDPSASTAFNHAVTYLGQPMS
                     SRFVDLSAAGVGQCLLLWEPGDNSPFEIESSYAFQTKPPDDSSRISFSLVPL"
     gene            1282..1569
                     /locus_tag="VFH83_01140"
     CDS             1282..1569
                     /locus_tag="VFH83_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HET6484990.1"
                     /translation="MVVSLIQFRLALPPMESIKDKRRIVSSIKTKLYQKFHLSVAEVD
                     LQDSMRYAQIGAAYVSNSKTFGESVLHKALRFVESNCEGMLEDAEVFSEVY"
     gene            complement(1606..2046)
                     /locus_tag="VFH83_01145"
     CDS             complement(1606..2046)
                     /locus_tag="VFH83_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484991.1"
                     /translation="MKSQLMEERIRRVSVLFSELSFRVIEDERSHNAYSATFEDDDAF
                     QAGVLIERGSRFLELAYALTFPSTHAQLIRGKLEEMLKTCYEFGCYVSLQQTASEINF
                     SVFSKVYYSGLNYYSLRDSLRDFRDCVGALMDLLDLGKDGSQER"
     gene            complement(2060..>2627)
                     /locus_tag="VFH83_01150"
     CDS             complement(2060..>2627)
                     /locus_tag="VFH83_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET6484992.1"
                     /translation="NHDKAVAGLMDLDWFNPVAREAALWTRRSMSEDLLDRLRRLPQG
                     PVRLDHGILLCHGTPMDEDAYLYDRASMQRTFECLDREHPQVRICLNGHTHVPLVIER
                     APGARSPRILGAPQEVAIDPEATYLINPGSVGQPRDGNPSASFGILDTTKLVYRNVRV
                     AYAVRETQRKIGMAGLPPQLGGRLADGR"
BASE COUNT          490 a          820 c          874 g          443 t
ORIGIN      
        1 ccagggggac caggctgaag gagatgcggg aggagtcgtc cggaggtttg gtctggaagg
       61 catacgagct ctcgatctcg aagggggagt tgtctcccgg ctcccaaagg aggagacact
      121 gccctacccc agcggcgctc aggtccacga accggctcga catcggctga cccaggtagg
      181 tcacggcgtg attgaaggcg gtcgatgcgc tggggtcgcg taccgtgccg ttatccttcg
      241 ggaggccgcc gacgccgatg gccggatccc ccacttcgaa tccggccttg cgatagtgga
      301 ccagcacccg gcccttcacg gggtttcgga gggacaccag cccctgcgtc gtgtccagga
      361 ccacgtcatc gaagcctgcc aggcgataga ggcggctccc gtccgtcgga ccggaacggt
      421 aggtcccctg gggatcctcg atgaatacct gcagcgtgtt cggatcgagc ccggtatccg
      481 gcaggaagaa gtaccggccc cggacgaagc tgtccagggg aacgtgctgc tccaccagct
      541 cgttcctacc gatgaacgtc ttctccttgc gccgggtgac atcccagcgg agaaggacgt
      601 cattcgtgga ggcaccggat tggaagctgg cctgggctcc cagagagccg gtcgcctggt
      661 tcggaaccgc gaggagtgga gtaggatcca tcgtgatgtc cgcggttccc acccgaacaa
      721 gctgcaccac ctcccccggg gcgccctggt accccaggtg gatcgcgtcg ctccccaggt
      781 ctccgacaac gttcgcgtcc acgaagaacc gtttcaacag ctcaagggat atggtaaggt
      841 ccggcgtctg gttgaagatg aacccctggt cgagctgggg gaaggcatcc agcggctgcg
      901 gcgccacgcc gggcagcagc aggaggccgg tcccaaagga ggcggtggcg gtccacgacc
      961 cgagcaggtc cagacggacg tccgcgccgc cgagctgggt cgagaggatc gtggtggggg
     1021 cctccggggg cgaggtgacg acctgagggg ttcccgcgtc cgtcggcggc ggcgcgtccg
     1081 gccagagagg gacggcgtgg gggaccgcgc agagaatcat cgcggccagg aggaggcgaa
     1141 tcggcagatg tcggggcacg cgaggcacag tattcaccag aaccgcccgt cctgcaaacc
     1201 gctgtctgaa tcacggcgcc gccaccaggg gctcaggcat aactataccg cgtgggaatg
     1261 cccgcgcgcg ggagcaaccg gatggtcgta tctctgatcc agttccgact cgccctgccg
     1321 ccgatggagt cgatcaagga caagcggcgg atcgtctcat cgataaagac gaaactgtac
     1381 cagaagttcc acctttcggt ggcggaggtg gacctccagg actcgatgcg atacgcccag
     1441 atcggagccg cctacgtctc caactccaag acgtttgggg aatcggtgct ccacaaggcg
     1501 ctgaggttcg tggagtccaa ctgcgaagga atgctggagg acgcggaagt gttctccgag
     1561 gtgtactgat tcgaccgcgc ggggcgccgc gccttcgcga cccgactacc gttcctgact
     1621 gccgtccttg cccaggtcga ggaggtccat gagggccccc acgcagtccc ggaagtcccg
     1681 aagactgtcg cggagggagt agtagttgag cccggagtaa taaaccttgc tgaacacgga
     1741 gaagttgatc tcactcgcgg tctgctgaag gctgacgtaa cagccgaact cgtagcaggt
     1801 cttcagcatc tcctccagct tgccccggat gagttgggca tgggtggacg gaaaggtgag
     1861 ggcgtacgcc agctccagga atctgctccc ccgctcgatc aggacgccgg cctggaacgc
     1921 gtcgtcatcc tcgaacgtgg cggagtacgc gttgtgggac cgttcgtcct cgatcacacg
     1981 gaaactgagc tcggaaaaga gcaccgatac ccggcggatc ctttcctcca tgagctggga
     2041 tttcatcacc cctcccctcc tacctcccat cagcgagccg gccgccaagc tgcgggggca
     2101 gcccggccat gccgatcttc cgctgagtct cgcgcaccgc gtacgcgaca cgaacgttgc
     2161 ggtagaccaa cttcgtggtg tctaatatac caaaagatgc cgacggattg ccatccctgg
     2221 gctgcccgac cgagcccggg ttgatgaggt acgttgcctc tggatcgatc gccacctcct
     2281 gaggagcgcc caggatccgg ggcgatcgcg cgccgggcgc tctttcgatc accaggggca
     2341 cgtgcgtgtg tccgttcagg cagatgcgca cctggggatg ctcccggtcc aggcactcga
     2401 aggtcctctg catggaagct cggtcgtaga ggtaggcgtc ctcgtccata ggggtgccat
     2461 gacagagaag gataccgtgg tcgagacgca cgggtccctg gggcagccgc cgcagtcgat
     2521 cgaggagatc ctcggacatc gatctccggg tccagagcgc cgcttcccga gccaccgggt
     2581 tgaaccagtc caggtccatc agcccggcta cggccttgtc atggttg
//
LOCUS       DASRZS010000050        17470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1569317, whole genome shotgun sequence.
ACCESSION   DASRZS010000050 DASRZS010000000
VERSION     DASRZS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 17470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17470
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569317"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VFH83_01155"
     CDS             complement(<1..1064)
                     /locus_tag="VFH83_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6484993.1"
                     /translation="MIDTIDLNGSWTLTWQDGERGPGKADGVMGDARARAPQSFSARV
                     PGEVHLDLMAAGILEEPTIGLNCLRARWVEESYWIYRRTFTLGALDAGVRAYLRFERL
                     ELAALVRLNGTEVGRHANAFLPACLEVTAALRSGENLLVVEIEAGLFGASDRPWEGWG
                     ANDGVRLTKRHWLRTVQSSAGWDWSQRLLNVGITGGVRLEICRGVRVDDCALIPELQE
                     SLEEGTLCGRVFVEGLDAGTRPARLTLDVRPLDSSADPLHEEATLAVAPGRQRLQVEA
                     RLLRPRLWWPRGQGEPSLYEARLTLRVGDRVLLDERRTVGFRRVKINQEKVSDGRLFV
                     IEVNGRPVFCKGGDFVPADP"
     gene            complement(1061..2050)
                     /locus_tag="VFH83_01160"
     CDS             complement(1061..2050)
                     /locus_tag="VFH83_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6484994.1"
                     /translation="MSEGSTPFILCENLVKIFKVEDVEVVALQGLDLTVGEREVVAII
                     GPSGSGKSSLMNLLGGLDTPSAGKIRVAGRDLVSMSAKERTRYRRDTVGFVWQNVSRN
                     LIPYLSALENVELPMILSGTFRRKQAHRLLEIVGLGKRKHHPPGRMSGGEQQRVAIAI
                     ALANNPALLLADEPTGSLDRENTRLLLNVFDTVRNELGVTVVIVTHDRSIARAVDRYL
                     EIRDGKTTREAVRKTSGLTPLDAVQEIHDPVGGGSPTASPETHEHYTLLDAAGRLQIP
                     PDLLESHGIKGRVRLVEEDGRIVILPEKQTSTEPAGPEVPRPEEDPGDERGTS"
     gene            complement(2047..4854)
                     /locus_tag="VFH83_01165"
     CDS             complement(2047..4854)
                     /locus_tag="VFH83_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6484995.1"
                     /translation="MYLVAILWITVKRIFHNWKVVGALLGGLVLAAGVMAAIPIYSAG
                     SLQSSFFREWMTQDSFRPPFTLIVTHTNERRGKAEALSSLNRMERSLDRDIRQAVGRA
                     PLARSDYASIGDCVLQPRDAQPDLSFSAPRAELSWMTGVGNLAQIAQGRWYQPRTDGI
                     VEVVVDETTLEQTGLLVGQKLTAWYELQDGELSSAAVNGAVPIPIEVVGFFRARPGFT
                     TKDWIYPPPFFNRLFVEPSVFRDDLMGRRGLRAERWDMEWVFSPEPVQVALLPGIISH
                     LEAAQRRATQVLPNTVYWLSPVDFFRSYNERADRVALFLAALATPLMGMILYYIVLMA
                     GVAVQHRSKEILVMHSRGAGRLQAVLSFFLEWLLLGAVAFLVGPFLGVFIARAMGASS
                     GFLSFVDREAIPAFASPQAYRFSLAASLIALAAGMGPVFATFRHTIVTFSRRGARRTR
                     SLWHRLFLDVILLAVAAVAYRELMWQSTRLAPDETVAADPILFFVPVVFLLGAGLLLL
                     RIYPFLLAGLRWISGWFRGVVWQLSLRRLTRNAVQYFPLLLLLILTLSLGIYSASSAR
                     TLSRNFDDRIRFEVGADLAVKEEWHPPDRPATGEPTSEPPFLKRLDLPGVLSAARVLK
                     DGVDVTVGGQWELANAGVLMAISPAEFEKTAWFRSDFAKAPLVDYLSLLARHHEGVIV
                     STKFYNDQQLTLGQELHFVFHEQPFDAYVAGVVDLWPTLDPSRSPFFILNIDYVLENI
                     RLEPYEVWYRLANHDHVQEMVTDMTDLGVYPTQVRDAEAMITALKHEPYRMGFFGVLS
                     LSFVIAALITVLGFLVYTYFSIRGRLLQFGALRAMGLSRLQLTLLLVLEQLFTLGAGL
                     AAGVFLGSLCSRLFLPFLRARAAELQPVPPFLLISDRTDILYVLAVLGALFVAAVVGL
                     TVILARLKINRALTLGEEVEP"
     gene            complement(4884..5918)
                     /locus_tag="VFH83_01170"
     CDS             complement(4884..5918)
                     /locus_tag="VFH83_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6484996.1"
                     /translation="MSNRRIVGRARSRILAAALSAGVAGLLAGCGFLPKEKAVPEPTL
                     PEPPTTTSAISYPVKRADIAEAVEGTAEVTSVRSVALYFKEPGRIKEINVSAMQKVAE
                     GDLLARLDIGDLEHELRLAQIDQQIAQVTRDRMNVVGAGAFDKRIQDLAIAKANETVS
                     YLQERVDASTIRAPFAGIVETVDAQPSDRVQDYSTIMELTDPTQLELQLKVTRQDYDM
                     IHPGQASEINSSQDQWTPTNVTEVTHLDPTRDVSVRVEQFIVHLRLPKGVPDSGMGRA
                     LAVRVVLQRHRNTLTIPLAALREFEGRTYVRVIEGDARHEQDVRVGIRTQTDAEILEG
                     LQEGQRVIGR"
     gene            complement(5915..6625)
                     /locus_tag="VFH83_01175"
     CDS             complement(5915..6625)
                     /locus_tag="VFH83_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6484997.1"
                     /translation="MTDLLLSQPQDPLVVLEGLSRFFAGAGERVRAVDGVSLTIQSGT
                     LVLIQGKSGSGKTTLLNLMGGLDTPTEGRIRLRGRELTRLSQRELSRLHRGEIGFVFQ
                     AFALMPGLTACENVDLPLRIAGCRAREAQERALRCLERVGLAKRAHHRTEELSGGEQQ
                     RVAIARGLVSQPRLLLADEPTGELDHAMVGTIMTMFREIVRESGMTICLTSHDPAVQP
                     YVDFRYTMADGRLAEEGA"
     gene            6799..7587
                     /locus_tag="VFH83_01180"
     CDS             6799..7587
                     /locus_tag="VFH83_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET6484998.1"
                     /translation="MEGLEKVKGKLASGELVLGTHVSLADSCVTELLGDVGFDLLWID
                     TEHSPLDKSGLVLHLVAARAAGAAAFVRVPWNDPVLAKPVLEMGVDGIIFPMIRTEEE
                     ARRAVSACLYPPEGIRGFGPRRAARYGLVGGADYLRAAASGFWRILQIEHHQAVDNLD
                     AILSVPGVDSIVVGPNDLSGSVGLLGQTGHPRVTELMDRIVETAHRRKVPVGTSIPED
                     PATIQQWIDRGVQWLVTGSDTSYLANGARSLFRTVRGMKRGGRP"
     gene            7584..8564
                     /locus_tag="VFH83_01185"
     CDS             7584..8564
                     /locus_tag="VFH83_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET6484999.1"
                     /translation="MKTDRPEGRKVMVTVSTFGQHSDARAKLEREGCTLVFPPKPPLK
                     EAALLEQLPSFNAIIAGGDEISAPVLSASTRLSIVARFGVGVDKVDVAAATRLGIWVG
                     IATNQEAVADLTLALLLALARRLREADAYVRAGSWGGFAGMDVYGRTIGVIGTGRIGQ
                     AVLRRARAFTPTVLASDPRQDPDAAARLGFTYVPLEELLRRSDFITVNCALTDGTRGL
                     IGEKELSLVKPTVCIVNTARAPIVVEGAIVKALREKRVAGFATDVFEPAPPPRDFPLF
                     VFPNVIVTPWFASYTPDTHRDMELACAENVLRVFRGEPPLYACNQVTPRG"
     gene            complement(8574..9407)
                     /locus_tag="VFH83_01190"
     CDS             complement(8574..9407)
                     /locus_tag="VFH83_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6485000.1"
                     /translation="MRLAFSTLACPDWSWSEAIRAARSYGYEGIEWRTIDGQLVTPSL
                     PNDTARAIASAMRSEGLETCALDASVQMALGPGEERQKAVQEVLEMLEVARALDAGFL
                     RVFIGKYPPQTPDATAIRWVADGLAEALPKASALGIGIALEVHSFEGRGKNVNGTSDS
                     TLCRQVVEACASPSLGILWDVGNPYLEEEPLSETWENVKGHLLYLHVKDQKRLADGAW
                     KYVLNGEGDVDLARMAALLRGQRFTGWLSYEWEKKWHPELADPGVALPHYVSAMRALL
                     K"
     gene            9452..10315
                     /locus_tag="VFH83_01195"
     CDS             9452..10315
                     /locus_tag="VFH83_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485001.1"
                     /translation="MTKKRVSASDVRPSDGAHLLLSTVTLLAALAFAVFFQMSKQPPF
                     REINPFGVDPYDAVGSIAFQVTLVLALLSFARSVRLRMDGPRPGVDGFPRAILRGVRL
                     AVLAILVTLITDCIAVLVNPVPRSRWGDLLLGQLAVLVLVVCGLLVWLAFAMARAPAA
                     VPSSLTPADAIDDLWSLVRALSALAVGSSRRRSVDPLVSDRLFSRLPWIDPRRHPWRF
                     TTLVGLLAGLLGWLGHLREGPPPSLSAGLLVAVISLGVETALAVVALAIVGGYLGLRP
                     SFARGVRRRVP"
     gene            complement(10296..12740)
                     /locus_tag="VFH83_01200"
     CDS             complement(10296..12740)
                     /locus_tag="VFH83_01200"
                     /inference="COORDINATES: protein motif:HMM:NF023636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HET6485002.1"
                     /translation="MGAQEGIRYRGSRLNHLAFPLGGLGAGMLCLEGNGGLNHVSIRN
                     RPELYHEPGLFASVHVRAPGAETGTARILQGPIQSWKIFGPGSDRGNGISGRAYGYPR
                     FAEAEFTARFPFGTVHLADKTLPLRADCTGWSPFIPGDADRSSLPVAALEYTLANPSD
                     QALEVLFGFHVPNFLSLPDAPAAITAAPGGFTLVGKEGQLGVRCLDGDVRVDHCWFRG
                     GWWDPATTLWDTIVSGRIRETQPVSQPPAPGASLWVKAALPAGATSWVRILLAWYFPT
                     TNLQVGDPSRGGDQGTCDCSPDGTHRPWYAGRYSGLSEVMTAWAEGYDGLRQESLRFS
                     DCLFSMEAPSEIIDAVSANLGILKSPTVLRDTNGRAWGWEGCFDSVGCCHGTCTHVWN
                     YAQAMAHLFPALERGLRETEFHCSQDSAGHQNFRTSLPIAYSDKRQIAASDGQLGGII
                     KVYREWRISADEAWLRRMWPAVKLSMDFCIRHWDPREKGVLSEAHHNTYDIEFWGPEP
                     MCSSLYLGALTAYAEMGAALGQDVSRYRRIAGRGRRYLERKLFNGEYFVQEIEYRDLK
                     TPYTVEDGAPPEAVERARREGPKYQYGTGCLSDGVIGAWLGQASGLPPFLAAPKVKRH
                     LKAVFAYNFRPKLLEHANPQRSGYALHDEGGLLLCSWPKGGMPSLPMVYSHEVWTGIE
                     YQVASHLLMNGLSRQALAIVKACRSRYDGTRRNPFDEYECGHWYARALASYALLQAAS
                     GARYDAADKTLFLSPNLRGDVMTFLCTATGYGLAGVRGGRPVLEVWQGSIDVRTVDYQ
                     GESVDASQVVVKAPGV"
     gene            12880..14283
                     /locus_tag="VFH83_01205"
     CDS             12880..14283
                     /locus_tag="VFH83_01205"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HET6485003.1"
                     /translation="MVDASAPEPLRIEGARALIRARIAASRTRVVVIDDDPTGTQTVH
                     GVRVLLSWPVPVLRSLLSSTDPLFYLCTNSRSLEAGAAARLSVQVGRALRTAARREDM
                     TVALLSRSDSTLRGHFPIEVESLASGLGLEPDGIIIAPAFFEAGRFTAEDVHWVERGG
                     TVVPACETEFARDPTFGFRSRDLRSWVEEKTHGAVKASSVLSLSLDLIRRSGPEGVAR
                     RLLEASRGQPVIVNAACYEDLEVTVLGILQAEAAGRRFVYRTAASFAKVRAGMDDRGL
                     LTEAELGVRRGPVLIVVGSYVEATTLQLTRLRESGIAEGVELRTSALESPGRLQRTWR
                     REIETAAGRVSALLASGRCAILYTSRERRETREFLGFGARLMAGLCDVVRRLSAKPGA
                     IVAKGGITSVAVARDALGVRQATVLGQVLPGVPVWRLGAGSRLADVPYVVFPGNVGGP
                     EALAQVVQRLTGSPRPE"
     gene            complement(14308..15420)
                     /locus_tag="VFH83_01210"
     CDS             complement(14308..15420)
                     /locus_tag="VFH83_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET6485004.1"
                     /translation="MKITEVRAIGLRGGTPKGGWTNELTPQECVQTLVSVTTDEGITG
                     WGSSFANDALVKGALAVLEPLYRGENALEPERVSEKLRQNTFWQGRGGSITHAISGID
                     IALWDILGKVSGQPVGRLLGGRYRDRVKPYASLLMDEPRILSRNLKEIARHGFRAFKI
                     GWGPFGRQSDALDEEIVRAAREAIGPEALLMVDAGGSDAFWSRGYKWALRTSEMLSRY
                     QVAWFEEALHPDALEDYVLLRQRSPVPISGGEVLTRRQAFQSWLRAGAFDIVQPDATK
                     AGGITESRRIGWMAEEYGARLIPHGWNTAVGLAADLQLASALAQTNLVEYLVGSPYVD
                     EIVQTPWALDADGMLAIPGKPGLGVELDLKAVARYS"
     gene            complement(15452..16270)
                     /locus_tag="VFH83_01215"
     CDS             complement(15452..16270)
                     /locus_tag="VFH83_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET6485005.1"
                     /translation="MENDEYRYNRLTWQEMNDAIRDQRVVLLPVGSTEQHGPHLPLDV
                     DSFLCESVCLEVGRRNPGLVLVLPTVSYGLNLHHIDFPGTIHIEPEVFIAFCLNVTKS
                     VAYHGFPKILVVNGHGSNTPLADLVARKTTLATSSLCAAVNYYSLAMEAFGRVKETET
                     IAHADEFETSLYLHLAPERVRMDRAVAGEDRNGQTLSSDSTSNYKVRFSDIWGRWTRT
                     GVHGDPRSATAEKGKVVFEAAVAGLAEVAAEWKAWPIEERSDQHALPAQKGIRW"
     gene            16342..17430
                     /locus_tag="VFH83_01220"
     CDS             16342..17430
                     /locus_tag="VFH83_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HET6485006.1"
                     /translation="MSGYNGLGLHLGNLSRVSDALTRSISAENPTGEPGKGGMAASGM
                     GGKAARELGRGWKLSPCVTIQGGQTFEVADIHGSGAIQHIWMTPSGAFRLSILRIYWD
                     DAPTPSVECPVGDFFACGWNQFAQVSSLPICVNPGSGLNCYWEMPFRSRCRITMTNIG
                     SEEMVLFYQVDYTLTEVPSDAAYLHAQFRRANPLPYKEVYTLLDGVVGRGQFVGTYMA
                     WGVHTTGWWGEGEIKFFLDDDDEFPTICGTGTEDYFCGSYGFEVNRGAGKAYGEFTTP
                     YAGMPQVIRPDGLYASQTRLSLYRWHIMDPIRFTKKLRVTMQALGWRSGGRYLPLQDD
                     IASVAFWYQTPQAASVPNLPDRDYLEII"
BASE COUNT         2855 a         5860 c         5830 g         2925 t
ORIGIN      
        1 atggggtccg cgggaacgaa gtcgcctccc ttgcagaaca cgggtcttcc gttcacttcg
       61 atgacaaaga gccgcccgtc ggagaccttc tcctggttga tcttcacgcg ccggaaccct
      121 accgtccgcc gctcgtccag cagcacccgg tcaccaaccc gaagcgtaag acgcgcctca
      181 tagagggacg gctcgccctg cccgcggggc caccagaggc gtggccgcaa gagccgggcc
      241 tcgacctgga gcctctgccg tcccggcgcc acggcaagcg tggcctcttc gtggaggggg
      301 tcagcggaac tgtccagcgg tcgaacatcc aaggtgagcc gggccggccg cgtgccggcg
      361 tccaggcctt cgacgaaaac ccttccgcaa agggttccct cctccagaga ctcctgcagc
      421 tcgggaatca gggcgcagtc gtccacccgc accccgcggc agatctccag acggactccg
      481 ccggtgatcc cgacgttgag gagccgttgg gaccagtccc atccagcaga agactgcacc
      541 gttcgcaacc agtgccgctt ggtgagccgg accccgtcgt tggcacccca cccctcccac
      601 ggccggtcgc tggcgccgaa gagccctgcc tcgatctcca ccaccagcag gttctcaccc
      661 gacctcaggg ctgcggtcac ctcgaggcag gccggcagga acgcgttggc atggcggccc
      721 acctcggttc cgttcaggcg gaccagggcc gcaagctcca ggcgctcgaa cctcaggtag
      781 gcgcgaaccc cggcatccag cgctccgagc gtgaacgtcc gacggtagat ccagtagctc
      841 tcctcgaccc atcgggccct caggcagttc agaccgatgg tcggctcctc caggattccg
      901 gccgccatga gatccaggtg cacctcgccg ggcactcgtg cggagaagga ctgaggagct
      961 cgcgccctgg catcgcccat tactccgtcc gccttgccgg gaccgcgctc tccgtcctgc
     1021 caggtaaggg tccacgagcc gttgaggtcg atggtgtcga tcatgacgtt cctctctcgt
     1081 cacccggatc ttcttccggg cggggtactt ccgggcccgc gggctcggtg gaggtctgct
     1141 tctccggcag gatgacgatc cgaccgtcct cctccaccag gcgaacgcga cccttgattc
     1201 catgggactc cagcaggtcc ggcggtatct ggagcctgcc cgcggcatcc aggagcgtgt
     1261 agtgctcatg ggtctccggg gacgccgtcg gagatccacc gcccaccggg tcatggatct
     1321 cctggacggc atccaggggc gtcaggccgc tggtcttgcg aaccgcctcc cgcgtcgtct
     1381 tcccgtctcg gatctccagg tagcggtcca cggcgcgcgc gatggaccgg tcgtgggtca
     1441 cgatgacgac cgtcacgcca agctcgtttc gcacggtgtc gaagacgttc aggagaagac
     1501 gggtgttctc ccggtccagg gaccccgtgg gctcatcagc cagaaggagc gccgggttgt
     1561 tcgccagggc tatcgcgatg gccacccgct gctgctctcc gccggacatg cgtcccggcg
     1621 ggtggtgttt ccgcttcccc agacccacga tctccaggag tcggtgcgcc tgcttccgac
     1681 ggaacgttcc ggagaggatc atcggaagct cgacgttctc cagcgcgctc aggtagggga
     1741 tgaggttgcg gctcacgttc tgccagacga accccactgt gtcgcgtcga taccgggtgc
     1801 gctccttggc gctcatggaa accaggtccc tgccggccac ccggatcttc ccggcagatg
     1861 gggtatccag acctcccagc aggttcatca ggctggactt gccgctgccg ctgggcccga
     1921 tgatggccac cacctctcgc tcacccacgg tgaggtccag tccctggagc gccaccacct
     1981 ccacgtcttc caccttgaag atcttgacga ggttctcaca gaggatgaac ggggtggaac
     2041 cctcgctcac ggctcgacct cttccccgag ggtgagcgcg cgattgatct tgagccgggc
     2101 caggattacg gtcaggccca ccacggccgc cacgaacaga gcgccgagaa cggccagcac
     2161 gtacaggatg tcggtccggt cggatatgag gaggaacggc ggcacgggct gcagctccgc
     2221 ggcgcgggcg cggaggaacg ggaggaagag acgagagcac agggaaccca ggaagacacc
     2281 ggccgccagt ccggcgccca gcgtgaagag ctgctccagc accaggagca gggtgagctg
     2341 gagacgggag agacccatgg cgcgaagcgc gccgaactgg aggagccggc cccggatgga
     2401 aaagtacgtg tacaccagga accccagcac ggtgatcaaa gccgcgatga cgaagctcag
     2461 ggacaggacg ccgaagaacc ccatgcggta gggctcgtgt ttgagcgccg tgatcatagc
     2521 ctccgcgtcg cggacctggg tgggatacac tccgaggtcc gtcatgtcgg tgaccatctc
     2581 ctggacgtgg tcgtggttgg ccagccggta ccagacctcg tacggttcca ggcggatgtt
     2641 ctccaggacg taatcgatgt tcaggataaa gaacgggctt cgggaggggt ccagggtagg
     2701 ccacaggtcc acgacgccgg ccacgtaagc gtcgaagggc tgctcgtgga atacgaagtg
     2761 cagctcctga ccgagcgtga gctgctggtc gttgtagaac ttcgtgctca cgatgacccc
     2821 ttcgtggtgc cgggccagga gggacaggta atcgaccagg ggcgccttgg cgaagtcgct
     2881 tcggaaccag gccgtcttct cgaactcggc cggggagatg gccatcagga ctcccgcgtt
     2941 ggccagctcc cactgccctc ccacggtcac gtcgactccg tccttcagaa cgcgcgcagc
     3001 cgaaaggacc cccggcagat ccagacgctt cagaaacgga ggctcggagg tgggctcgcc
     3061 ggtggcgggt cgatccggag ggtgccactc ctccttgacc gccaggtccg ccccgacctc
     3121 gaagcggatg cggtcatcga agttccgcga gagcgttcgg gcgctggagg cgctgtagat
     3181 gccgagggaa agggtcagga tgaggaggag caacagggga aagtactgca cggcgttccg
     3241 cgtcaggcgg cgaagggaga gctgccagac cacgccgcgg aaccatcccg agatccagcg
     3301 cagcccggcg agcaggaacg gatagatcct cagaagcagc aggcctgccc ccaggagaaa
     3361 gacgactgga acgaagaaga ggatggggtc ggccgccacc gtctcgtccg gcgccagccg
     3421 cgtggactgc cacatgagct ctcgataggc cacggcggca accgccagga ggatgacgtc
     3481 caggaagagc cgatgccaca aggagcgggt ccgacgggcc ccgcgtcggc tgaacgtaac
     3541 gatggtgtgg cggaaggtag cgaagaccgg ccccatcccg gccgccaacg caatgaggga
     3601 tgccgccagg gagaaccggt aggcctgcgg gctggcgaac gccggaatgg cctcacggtc
     3661 cacgaaggag aggaaacccg aggaggcgcc catggcgcga gcaatgaaca cgcccaggaa
     3721 cggcccgacc aggaaggcca cggcgcccag gagaagccac tccaggaaga agctcagcac
     3781 ggcctggagc cttcccgcgc ctcggctgtg catgaccagg atctccttgc ttcgatgctg
     3841 caccgcgaca cccgccatga gaacgatgta gtagaggatc atccccatga gcggcgtggc
     3901 cagggccgcc aggaagaggg ccacgcggtc ggcccgctcg ttgtaggagc ggaagaaatc
     3961 cacgggggag agccagtaga ccgtgttggg aaggacctgg gtggcgcgcc gttgggctgc
     4021 ctccaggtga gagatgattc ccggcaggag agctacctgg acgggctccg gagagaagac
     4081 ccattccatg tcccagcgct cggcgcgcag cccacggcga cccatgaggt cgtcgcgaaa
     4141 taccgagggc tccacgaaca gccggttgaa gaagggcgga gggtagatcc agtccttggt
     4201 ggtgaacccg ggtctggcgc ggaagaagcc caccacctcg atcggaatgg gaacggcgcc
     4261 gttcacggct gcgctcgaaa gctcgccgtc ctgaagctcg taccaggcgg tcaacttctg
     4321 gcccaccagc agcccggtct gctccagggt ggtctcgtcc accaccacct ccacgatccc
     4381 gtcggtgcgc ggctggtacc agcgtccctg cgcgatctgc gccaggttcc ccaccccggt
     4441 catccagctc agctccgccc gcggagcgga gaacgagagg tccggttggg catcccgggg
     4501 ctgaaggacg cagtccccaa tgctggcgta gtccgatcga gccaggggcg cgcggcccac
     4561 cgcctggcgg atgtcgcggt ccagggagcg ctccatccgg ttgagcgagg agagcgcctc
     4621 cgccttcccg cgccgttcgt tggtgtgagt gacgatgagc gtgaacggcg gacggaagga
     4681 gtcctgggtc atccactcgc ggaagaagct ggactggagc gaaccggcgg agtagatggg
     4741 gatggcggcc atgacgccgg ccgccaggac caggccgccc aacaaggcgc cgacgacctt
     4801 ccaattatgg aagatcctct tcaccgtgat ccacagaatg gccaccaggt acacgggcct
     4861 gccctccgct ccacgcggcc gccctagcgg ccgatcaccc gctggccttc ctgcaggcct
     4921 tccaggatct ccgcgtccgt ctgggtccgg atccccaccc gaacgtcctg ctcgtggcgc
     4981 gcgtctccct cgatcacgcg cacgtacgtc cttccctcga actcgcgaag cgcggccagc
     5041 ggtatggtga gcgtgttacg gtgacgctgg agcaccaccc ggacggccag ggcgcgaccc
     5101 atcccggagt ccgggacacc cttgggcagg cgcagatgga cgatgaactg ctcgacgcga
     5161 acggagacgt cccgggtggg gtccagatgg gtcacctcgg tgacgttcgt gggcgtccac
     5221 tggtcctggc tgctgttgat ctccgaggcc tgtcccgggt ggatcatgtc gtagtcctgg
     5281 cgcgtcacct tgagctgcag ttcgagctgc gtggggtcgg tgagctccat gatcgtggag
     5341 tagtcctgga cccggtcgct gggttgcgcg tccaccgtct ccacgatacc ggcaaagggc
     5401 gcccggatgg tggaggcatc cacgcgctcc tggaggtagc tcaccgtctc gttggccttg
     5461 gcaatggcca ggtcctggat ccgcttgtcg aaggcgccgg cccctaccac gttcatccgg
     5521 tcccgggtca cctgcgcaat ctgctgatcg atctgggcga gccgaagctc gtgctccagg
     5581 tccccgatgt ccagccgcgc caggaggtct ccttccgcca ccttctgcat ggcggacacg
     5641 ttgatctcct tgatccggcc cggctccttg aagtagagag ccaccgagcg gacggaggtc
     5701 acctccgcgg ttccttccac cgcctccgct atgtctgcgc gtttcaccgg atacgaaatg
     5761 gcgctggtag tggtcggagg ctcggggagc gtgggctcgg gcacggcctt ctccttcggg
     5821 aggaatccgc agccggccag gaggcccgct accccggcgg agagtgctgc cgcgagaata
     5881 cgagagcgcg ctcgacccac gatgcgccgg ttgctcacgc gccctcctcc gccaggcggc
     5941 cgtccgccat cgtgtagcgg aagtccacgt agggctgcac ggcaggatcg tggctcgtca
     6001 gacagatcgt cattccactc tcccgaacga tctctcggaa catcgtcatg atcgtcccga
     6061 ccatggcgtg gtccagctca ccggtcggct cgtccgccag aaggagacgc ggctggctga
     6121 ccagtcctcg agcaatcgcc acgcgctgct gctcgccgcc gctcagctcc tccgtgcggt
     6181 ggtgcgcccg cttggccaga cccacgcgct ccaggcatcg aagcgcccgc tcctgcgcct
     6241 ctcgcgcccg acagcctgcg atccgaagcg gaaggtccac gttctcgcac gcggtcaggc
     6301 ccggcatgag ggcaaacgcc tggaacacga acccgatctc gccgcggtgg agacgggaca
     6361 gctcgcgttg ggacaatcgc gtcagctccc ggcctcggag ccggatccgg ccctccgtag
     6421 gcgtgtccag cccgcccatg aggttgagga gcgtggtctt gccggacccg gacttgccct
     6481 ggatcaggac cagggtcccc gactggatcg tgaggctgac cccgtccacg gcgcgcacgc
     6541 gctctcccgc acccgcaaag aagcgagaca atccctcgag gacgaccaga gggtcctgag
     6601 gctggctgag caggagatcg gtcatgctgg gttccagggc tgaaccctgg cagcttatca
     6661 ggatccgagg ggttttggaa tcagcgtcgt ggagcgctcg gaacgccgac gctcgggagc
     6721 cgcgccgact ggcaccggcg cctacgttcg ggtagattgt gccaagggtt gcttgcctcc
     6781 cggaggtgcg ataaggcgat ggaaggactg gagaaggtca agggaaagct ggcgtcggga
     6841 gagctcgtcc tgggaaccca cgtcagcctg gcggactctt gcgttaccga gctcttgggg
     6901 gacgtggggt tcgatctcct gtggatcgat accgagcact cgcccctgga caagtcgggg
     6961 ctggtgctcc acctggtggc ggcccgagcc gcgggagcag ccgccttcgt tcgcgtgccg
     7021 tggaacgatc cggtcctggc caaaccggtg ctggagatgg gcgtcgacgg gatcatcttc
     7081 ccgatgatcc gcaccgagga ggaggcccgc cgcgccgtgt ccgcctgcct gtacccgccg
     7141 gaaggcatcc gcggcttcgg cccgcggcgc gccgcgcgat acggcctggt gggcggagcg
     7201 gactaccttc gcgcggcggc aagcggtttc tggcgcatcc tccagatcga gcaccaccag
     7261 gccgtggaca acctggacgc catcctctcg gtgcccgggg tggactccat cgtagtgggt
     7321 cccaacgacc tctccggttc cgttgggctc ctcggccaga ccggtcatcc tcgggtgacg
     7381 gagctcatgg accgcatcgt cgagaccgct catcgcagaa aggtgccggt ggggacctcc
     7441 atcccggagg atcccgccac gatccagcag tggatcgacc gcggggtgca gtggctggtc
     7501 acgggctccg acacttccta cctggcaaac ggagctcgtt cgctctttcg aacggttcgc
     7561 ggcatgaagc gaggaggcag accatgaaga cggaccgccc agaaggccgg aaggtgatgg
     7621 tcacggtttc gacctttggc cagcactcgg atgcgcgggc gaagctggaa cgcgaaggct
     7681 gcacgctggt gttcccgcca aagccaccgc tcaaggaggc cgcgctgctc gagcagcttc
     7741 cctcgttcaa cgcgatcatc gccggcgggg acgagatctc ggccccggtc ctctcggcct
     7801 cgacccggct cagcatcgtc gcgcgattcg gggtcggcgt ggacaaggtg gacgtggccg
     7861 ctgccacccg cctgggaatc tgggtcggga tcgccaccaa ccaggaagcc gtcgcggacc
     7921 tgacgcttgc ccttctcctg gcgctggcgc gaaggctgcg ggaggctgac gcctacgtcc
     7981 gggccggttc ctggggagga ttcgccggaa tggacgtcta cggaaggacc atcggggtga
     8041 tcggcaccgg gaggatcggg caggcggttc tccgtcgagc gcgggcgttc acgccgacgg
     8101 tgctggccag tgacccccgg caggatccgg acgcggcggc tcgactcggc ttcacctacg
     8161 ttccgctgga ggagctcctg cgcaggtcgg atttcatcac cgtcaactgc gcgctgaccg
     8221 atgggactcg gggactgatc ggagagaagg agctttccct cgtcaagccg accgtctgca
     8281 tcgtcaacac cgctcgggcg cccatcgtcg tggagggagc catcgtgaaa gcgctccgag
     8341 agaagcgggt cgcgggattc gctacggacg tattcgagcc ggcgcctccg cctcgggatt
     8401 ttccgctctt cgttttcccc aacgtcatcg ttaccccgtg gttcgcgtcc tatacgccgg
     8461 acacccaccg ggatatggag cttgcctgcg cggagaacgt gctccgagtg tttcggggtg
     8521 agccgcccct gtacgcgtgc aaccaggtta ccccaagagg gtgatccggg aggtcacttc
     8581 agcagcgcgc gcatcgccga cacgtagtgc ggcaaggcta ccccgggatc cgccagctcg
     8641 ggatgccact tcttctccca ctcataggag agccatccgg tgaaccgctg cccccgcaga
     8701 agcgccgcca tgcgcgccag gtctacgtcg ccctcaccgt tgagcacgta tttccacgcc
     8761 ccatcggcca ggcgcttctg gtctttcacg tggaggtaca ggagatggcc cttcacgttc
     8821 tcccaggtct cggagagcgg ctcctcctcg aggtagggat tgccgacgtc ccagaggatg
     8881 ccgagggagg gggaggcgca tgcctccacg acctgacgac agagggtgct gtcgctcgtc
     8941 ccgttcacgt tcttgccgcg accctcgaag gagtgcactt ccagcgcgat gccgataccc
     9001 agcgccgaag ccttggggag cgcctcggcg agcccatccg ctacccaccg aatggccgtg
     9061 gcgtccggcg tctggggcgg gtacttcccg ataaaaacgc gtaggaatcc cgcgtccagc
     9121 gccctggcca cctcaagcat ttccaggact tcctggacgg ccttctgccg ttcctctccc
     9181 ggtcccaggg ccatctgtac gctggcatcc agggcgcagg tttccaggcc ttccgagcgc
     9241 atcgcgctcg cgatggctcg cgcggtgtcg ttcggaaggg acggggtcac cagctgtccg
     9301 tcgatggttc gccattcgat cccctcgtac ccgtaggatc gggctgcccg aatggcctcg
     9361 ctccagctcc agtccggaca ggccagggtc gagaatgcca gtcgcatgcg tgcctcctgt
     9421 cgtgaatgcc gggtcatgct acagtcccgg cgtgacaaag aaaagagtct cagcctcgga
     9481 tgtccgaccc tccgacggcg cgcatcttct tctgagcacc gtcaccctgc tcgcggccct
     9541 ggcgtttgcc gtcttcttcc agatgagcaa gcagccgccg tttcgagaga tcaacccgtt
     9601 cggcgtcgac ccctatgacg cggtcggctc catcgcgttc caggtcacgc tggtcctcgc
     9661 cctcctgagc ttcgcgcgat ccgtccgcct gaggatggac gggccgcgcc cgggcgtcga
     9721 cggcttccct cgtgccatcc ttcgaggggt acggctcgcg gtgctcgcca tcctggtgac
     9781 gctcatcacg gactgcatcg cggtcctggt caaccctgtc ccgcgctccc ggtggggcga
     9841 ccttctcctg ggtcagctcg cggtgctggt gctcgtagtc tgcggtctcc tggtctggct
     9901 ggcgttcgcc atggcgcggg caccggctgc ggtgccctcc tcgctgaccc ctgcggatgc
     9961 catcgatgac ctgtggtccc tggttcgagc cctgtcggcg ctcgccgtgg gctcatcgcg
    10021 gcggcgatcg gtcgatcccc tcgtgagtga ccgtctcttc tcgcggctgc cgtggatcga
    10081 ccctcggcgg catccgtggc gcttcacgac cttggtcggg ctgctggccg gtttgcttgg
    10141 ctggctgggt cacctgcgcg aaggtccgcc gcccagcctg agcgccggtc tcctcgtggc
    10201 cgtcatctcc ctgggcgtcg agacggcgct ggccgtcgtc gccctggcga tcgtgggcgg
    10261 gtatctgggc ctgagaccct cgttcgcccg cggcgtcaga cgccgggtgc cttgaccacc
    10321 acctgggaag catccaccga ctcgccctgg tagtccacgg tgcgcacgtc gatgcttccc
    10381 tgccagacct ccaggacggg ccgccccccg cgaacgcctg ccagaccata gccggtggcc
    10441 gtgcacagga aggtcatcac gtctcccctc aggttgggcg agaggaagag cgtcttgtcc
    10501 gcggcatcgt atcgggcgcc ggaggcggcc tggagcaggg cataggaggc cagggcccgc
    10561 gcgtaccagt ggccgcactc gtactcgtcg aagggattgc gccgggtgcc gtcatagcgg
    10621 gaacgacacg ccttgacgat ggcgagcgcc tgccgggaga gaccgttcat gagcaggtgc
    10681 gaggcgacct gatactcgat tcccgtccag acctcatggc tgtagaccat cggaagggat
    10741 ggcatgccgc ccttgggcca gctgcacagg agcagcccgc cctcgtcgtg cagggcgtag
    10801 ccggatcgct gcgggttggc gtgctccagg agcttgggtc ggaagttgta ggcgaacacc
    10861 gccttcaagt ggcgtttcac cttgggcgcg gccaggaacg gaggcagccc ggacgcctgt
    10921 cccagccacg cgccgatgac accgtccgag aggcatccgg tcccgtactg gtacttcggc
    10981 ccttcgcgtc gggcgcgttc gacggcttcg gggggggcgc cgtcctcaac cgtgtacggc
    11041 gtcttcaggt cccgatactc gatctcctgg acgaagtact ccccgttgaa gagcttgcgc
    11101 tcgaggtatc ttctgcctct gccggcgatc cggcgatacc gcgagacgtc ctggccgagc
    11161 gccgctccca tctccgcgta cgcggtgagc gcaccgaggt acaggcttga gcacatgggc
    11221 tcgggacccc agaactcgat gtcgtaagtg ttgtggtgcg cctcactcaa gacacccttc
    11281 tcccgagggt cccagtgacg gatgcagaag tccatgctga gcttgaccgc gggccacatc
    11341 ctgcgcagcc aggcttcgtc cgccgagatc cgccactccc gatacacctt gatgatccct
    11401 ccgagctggc cgtcggaggc cgcgatctga cgcttgtccg agtaggcgat gggaagcgag
    11461 gtccggaagt tctggtgtcc cgcggagtcc tggctgcagt ggaactcggt ctcccgaagc
    11521 cctcgctcga gcgccgggaa gaggtgcgcc attgcctggg cgtagttcca gacgtgggtg
    11581 caggtcccgt ggcagcagcc gacggaatcg aagcagcctt cccaccccca tgcccggccg
    11641 ttcgtgtccc gaagcaccgt gggggatttc aggatgccga ggttggcgga gaccgcgtcg
    11701 atgatctccg agggtgcctc catcgagaag agacagtcgg agaaccgaag gctctcctga
    11761 cgcagcccgt cgtagccctc ggcccacgcg gtcatcactt cggagaggcc ggagtatcgc
    11821 cccgcgtacc agggccggtg cgtgccatcc ggggagcagt cacaggtccc ctggtccccg
    11881 ccgcgcgagg gatctcccac ctggaggttc gtggtgggga aataccacgc caggaggatc
    11941 cggacccagc tggtggcgcc ggcgggcaga gccgccttta cccagaggct cgcgcccggc
    12001 gccggcggct ggctcaccgg ctgcgtctcg cgaatccggc ccgagacgat ggtatcccag
    12061 agcgtggtgg cggggtccca ccacccgccg cggaaccagc agtgatccac gcgtacatcg
    12121 ccatccaggc agcggactcc gagctgtcct tccttgccga cgagggtgaa gccccccggt
    12181 gccgccgtga tggcggccgg ggcgtccggc aaggagagga agttggggac gtggaacccg
    12241 aagagcacct ccagggcctg gtcgctcggg ttcgccaggg tgtactccag ggccgcaacc
    12301 ggcaggctgg accggtcggc gtcccccggg atgaacgggc tccagccggt gcagtcggcg
    12361 cgcaggggca gggtcttgtc cgccaggtgg accgtgccga aggggaatcg cgcggtgaac
    12421 tccgcttccg cgaatcgcgg gtatccgtat gccctgcccg agatcccgtt gcctcggtcg
    12481 cttccgggac cgaagatctt ccagctctgg atgggtccct ggaggatcct tgccgtcccg
    12541 gtctcggcgc caggggcccg aacgtgtacg gaggcaaaga gacccggctc gtggtaaagc
    12601 tcgggcctgt tgcggatcga cacgtggttc agcccaccgt tgccttccag gcaaagcatg
    12661 ccggccccca ggcctccgag cgggaacgcg aggtggttga gccgggatcc ccggtatcgg
    12721 atgccttcct gagctcccat gtagtcctcc tcgagggatt caaggcgtgc gcgagcctgt
    12781 ccatgatagg cattccggat gcagccgatc aagcggcctg gtgattgcgg tcgacgaggg
    12841 attgccgcga ccggcaatcc atgataggac gcgtggacca tggtggacgc cagcgctccg
    12901 gagcccttgc gcatcgaagg ggcccgcgcc ctcatccggg ctcgcatcgc ggcctcacgg
    12961 acccgcgtcg tcgtaatcga cgacgaccct acgggcacgc agacggtgca cggggtccgg
    13021 gtgctcctct cctggccggt gccggtgctc cgctcgctgc tttcgagtac ggatcccctg
    13081 ttctacctct gcaccaactc gagaagcctc gaggcaggcg ccgcggcgcg cctgagcgtc
    13141 caggtggggc gcgcccttcg gacggccgcg cggcgggaag acatgacggt agccttgctc
    13201 tctcgaagcg attccacgct ccgcggccac ttccccattg aagtggagag tctcgcctcc
    13261 ggtttgggcc tggaaccgga cggcatcatc atcgcccccg ccttcttcga ggcgggccgg
    13321 ttcaccgcag aggacgtgca ttgggtggaa cgcggcggaa cggtggtccc ggcgtgtgag
    13381 acggagttcg cgcgggatcc cacgttcggg ttcaggagcc gggacctgcg ttcctgggtg
    13441 gaggagaaga cccatggggc ggtgaaggcc agctcggtgc tttccctttc cctcgacctc
    13501 atccggcgat ccggccccga gggcgtggcc cggaggctcc tggaggcgtc ccgaggtcag
    13561 cccgtgatcg tcaacgccgc ctgctacgag gacctcgaag tgacggtcct cggcatcctc
    13621 caggcggagg ccgccggcag gcgcttcgtc tatcggaccg cggcgtcctt cgccaaggta
    13681 cgagccggca tggacgatcg aggcctcctc acggaggcgg aattgggtgt gcgcagggga
    13741 ccggtcctga tcgtcgtggg ctcctacgtg gaggcgacca cgctccagct cactcgcctc
    13801 cgggagtccg ggatcgccga aggcgtagag ctgcgtacca gcgcgctgga gagtccggga
    13861 aggctccaac ggacgtggcg cagggagatc gagaccgcag ccggcagggt gtccgctctc
    13921 ctcgcctccg gacgctgcgc aatcctctac acctcccgcg agcgtcggga gacgagagag
    13981 ttcctcggct ttggcgcgag gcttatggcg gggctctgcg acgtcgtgcg tcggctttcc
    14041 gcaaagccgg gcgcaatcgt tgcaaaggga ggcatcacca gcgtggccgt ggcccgggac
    14101 gcactggggg tgcggcaggc aacggtgctg gggcaggtcc taccgggggt tcccgtgtgg
    14161 cgcctgggag ccggctcccg ccttgccgat gtgccctacg tcgtgttccc gggaaatgtc
    14221 ggcggcccag aggctctggc ccaggtcgtc cagcgcctga cggggtcacc gcgtccggag
    14281 tagcgggcca tggccgccaa ggcgcgctca ggagtagcgg gccaccgcct tcagatcgag
    14341 ctccactccg aggccgggtt ttccgggaat ggcgagcatg ccgtcggcgt ccaacgccca
    14401 cggagtctga acgatctcat ccacgtaggg cgagccaacg aggtactcca ccaggttcgt
    14461 ctgggcaagc gccgaggcca actggaggtc cgcggccaga cccacggcgg tgttccaccc
    14521 gtgcgggatg agccgggctc catactcttc ggccatccac ccgatgcggc gggactccgt
    14581 gatccccccg gccttcgtgg cgtccggctg aacgatgtcg aacgcgcctg cccgaagcca
    14641 actctggaac gcctggcgcc gcgtcagcac ctcgccgccg gagatcggga ccggggaccg
    14701 ctgccgcagg agcacgtagt cttccagcgc atccgggtga agcgcctcct cgaaccatgc
    14761 cacctggtaa cgggagagca tctccgaggt ccgtagcgcc cacttgtagc cgcgggacca
    14821 gaaggcatcg cttccgccgg cgtccaccat gaggagcgcc tccggaccga tggcctcccg
    14881 ggcggccctc acgatctcct cgtccagcgc atcgctctgc ctgccgaagg gaccccaccc
    14941 gatcttgaag gctcgaaacc cgtgtcgggc gatctccttc aggttgcggc tcaggatgcg
    15001 tggctcatcc atgaggagag acgcgtaggg cttgacccgg tcccggtacc ttccccccag
    15061 gaggcgtccc acgggttggc cggacacctt gccgaggatg tcccagaggg cgatgtcgat
    15121 gccgctgatg gcgtgagtga tggaccctcc ccggccctgc cagaacgtgt tctgccgaag
    15181 cttttcgctc acgcgctcag gctcgagcgc gttctcccca cggtacaacg gctccagaac
    15241 ggcaagcgcg cctttgacaa gggcatcgtt cgcaaaggag cttccccacc cggtaatccc
    15301 ctcgtccgtg gtcacggata cgagggtctg gacgcactcc tgcggcgtga gctcgttcgt
    15361 ccacccgcct ttcggcgtgc cgccgcgcag cccgatggcg cgcacttccg tgatcttcac
    15421 cggctcatcc ctcctgctac ccctgacacc cttaccaccg gattcccttc tgggccggga
    15481 gcgcgtgctg gtccgagcgc tcctcgatgg gccacgcctt ccattccgcc gccacctccg
    15541 ccagccctgc caccgcggcc tcgaagacga ccttgccctt ctccgcggtt gcgctccgag
    15601 gatctccgtg gacgccggtc cgcgtccagc gtccccagat gtcgctgaac cggaccttgt
    15661 agttggaggt gctgtcggag ctgagggtct gcccgtttcg gtcctccccg gcaaccgccc
    15721 ggtccatgcg cacccgttcg ggcgccaggt ggagatagag ggacgtctcg aactcatcgg
    15781 cgtgcgcgat cgtctcggtt tccttgacac ggccgaaggc ttccatggcc agagagtagt
    15841 agttcaccgc ggcacagaga gaggaggtgg ccagcgtggt cttccgcgcc acgaggtccg
    15901 ccaggggcgt gttggatccg tggccattga cgacgaggat cttcggaaag ccatggtacg
    15961 ccacgctctt ggtgacgttg aggcagaacg cgatgaagac ctcgggctcg atgtggatcg
    16021 taccgggaaa gtcgatgtgg tgaaggttga gcccgtagga gacggtagga agcacgagca
    16081 ccagcccggg attccttcgc cccacctcca ggcacacgga ctcgcagaga aagctgtcca
    16141 cgtccagcgg gagatgggga ccgtgctgct ccgtggaacc gacggggaga aggacgaccc
    16201 tctggtcgcg gatggcgtcg ttcatctcct gccaggtcag ccggttgtag cggtattcat
    16261 cgttctccat ggcgatccct ccacctaccc accatacgct cctccttgac gccaatccag
    16321 cagcgggtca aggtgggagc catgagcggc tacaacggtt tgggactgca cctggggaac
    16381 ctctcgcgag tctccgacgc gctgacgcga tccatcagcg cggagaaccc cacgggggag
    16441 ccgggcaaag gaggcatggc tgcctcgggt atgggcggca aggctgctcg ggagctgggt
    16501 cggggatgga agctgtcccc ctgcgtcacg atccagggcg gacagacctt cgaagtcgcc
    16561 gacatccacg gatccggcgc catccaacac atctggatga ccccctccgg agcctttcgg
    16621 ctcagcatcc ttcggatcta ctgggacgac gcccccacgc cgtcggtgga gtgtcccgtc
    16681 ggggacttct ttgcctgcgg gtggaaccag ttcgcccagg tctcgtccct tccgatctgc
    16741 gtgaacccgg ggagtggtct gaactgctac tgggagatgc ccttccggtc ccgttgtcgg
    16801 atcaccatga ccaacatcgg atcggaagag atggtgctct tctaccaggt ggactacacg
    16861 ctcaccgagg taccttccga cgccgcgtat cttcacgccc agttccgcag ggccaacccg
    16921 ctgccataca aggaggtcta caccctcctc gacggcgtag tcggtcgggg gcaattcgtc
    16981 ggcacctata tggcgtgggg cgtccacacc accggctggt ggggagaggg agagatcaag
    17041 ttctttctgg acgatgacga cgagttccct accatctgcg gaacgggaac ggaagactac
    17101 ttctgcgggt cgtacggctt cgaggtgaac cggggggcgg ggaaggcgta cggggagttc
    17161 accaccccct acgcgggcat gccgcaagtg atccggccgg acggcctgta tgcgtctcag
    17221 acccggttga gcctctaccg ctggcacatc atggacccga tccggttcac gaagaagctc
    17281 cgggtgacca tgcaggcgct ggggtggagg agcggcgggc ggtaccttcc cctgcaggac
    17341 gatatcgcct cggtcgcgtt ctggtaccag acgccgcagg ccgcctccgt tccgaacctc
    17401 ccggaccggg attacctgga gatcatctga gcgggacgcc ccgcgctcag ggcgccggtc
    17461 cccgcggagg
//
LOCUS       DASRZS010000051         3496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5034838, whole genome shotgun sequence.
ACCESSION   DASRZS010000051 DASRZS010000000
VERSION     DASRZS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5034838"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="VFH83_01225"
     CDS             complement(<1..1219)
                     /locus_tag="VFH83_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6485007.1"
                     /translation="MKRLMVVLLVLAVAGTVFAQGKTLRVYMQGLTPREKLESERVAP
                     PTYLWTAAKDWQAKHPGWTIEFVPEITSGYDEWFTTQLVGGDAPDIIWNQRGWIQRDY
                     KKNWFADLTPVLNQKDPYVPSAASWYDTFLTPAIESGRAPDGKIYLITGDIVGTGIFY
                     NKDLFAKAGITSEPQTWADFMAASKKLKAIGVIPFATSMSKDGGIQLYGSWFSREIQD
                     VLYDSMMDQIKGAKVVRTWKPGENLPTKAMVNAIVKGRYAATQPQFKDMLRILKDWSQ
                     YWPDGFWAINTDANQVYDMWAKGQAAMGWFGSWITKTTLNDPLINFKWGILKKIPSIT
                     KATSQYGGSDFPAMAGVGGVVQYSIPQVTKDRGSFDMAVDWLRYVTSPTVLVAALNDH
                     GGFAPAVKDTKGAS"
     gene            complement(1288..2115)
                     /locus_tag="VFH83_01230"
     CDS             complement(1288..2115)
                     /locus_tag="VFH83_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485008.1"
                     /translation="MRRASLHDLPIHFFLVLFGIITFYPFVFTILTSFKTSYQISHNF
                     WGLPTSAELSNYRDAWTRLYPYLLNSLIVTSATVVGVLFVSSLAAYVFARFDFPGREF
                     LFILIICLLMIPSILLLVPQFLMVKSLRLLNTRWALILPYIAGGQVFAIFILRSFMEA
                     IPNELFDAGSIDGLSTVGAYRHIAIPMSIPVLSTVAILNILGTWNDYVWPLVTISKTF
                     LWTISVGIASFAQRTGSFETRGVMFAGYVIASAPLVILFFFTMRSFIKGISSGALKV"
     gene            complement(2121..3002)
                     /locus_tag="VFH83_01235"
     CDS             complement(2121..3002)
                     /locus_tag="VFH83_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485009.1"
                     /translation="MVKRRECYLLLVPTFALLGIFNYYPAASAIYHSFFNWNGANVQL
                     FNGFANFTDLFSNDDFLFSLVNVLKITIVSILISITFPLLAAVLIYRLRADRAAYVYK
                     VLYVIPLVVPSVVIFLIWKFIYSPTDGILNMLFNGMGLKGLTRTWLGEYSTALYAILF
                     VGFPWVSGFQTLIYLAGLQNISVSITEAATLDGTGSLRRFFSIELPLIASQVKLILIL
                     GMINAIQSFVSILIMTNGGPGKATMVPGLYLYRNAMQYGKMGYACSIGTLLFVVILIL
                     TYINLKYMKSNTDAMGG"
     gene            3292..>3496
                     /locus_tag="VFH83_01240"
     CDS             3292..>3496
                     /locus_tag="VFH83_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET6485010.1"
                     /translation="MRYDKLTVKAQEALQEADSLAHTFSHPALEVEHLLLALVEQKDG
                     VVPPILERIGADASEVSSAIRHAL"
BASE COUNT          695 a         1008 c         1166 g          627 t
ORIGIN      
        1 cgctggcgcc cttggtatcc ttgaccgccg gggcgaagcc gccgtggtcg ttgagggccg
       61 cgacgaggac cgtcgggctg gtgacgtagc gcagccagtc cacggccatg tcgaacgacc
      121 cgcggtcctt cgtgacctgg gggatggagt actggaccac gccgccgacg ccggccatgg
      181 ccgggaagtc cgacccgccg tactgcgagg tggccttggt gatgctgggg atcttcttca
      241 ggatgcccca cttgaagttg atgagggggt cgttcagcgt ggtcttggtg atccagcttc
      301 cgaaccagcc catggccgcc tggcccttgg cccacatgtc atagacctgg ttggcgtccg
      361 tgttgatggc ccagaacccg tcgggccagt actgggacca gtccttgagg atgcgcagca
      421 tgtccttgaa ctgcggctgc gtggcggcgt acctgccctt gacaatggcg ttcaccattg
      481 ccttggtggg aaggttctcc cccggcttcc acgtccggac taccttggcg cccttgatct
      541 gatccatcat ggaatcgtag aggacgtcct ggatctctcg gctgaaccag cttccgtaga
      601 gctggatgcc gccatccttg gacatgctgg tggcgaaggg gatgacgcca atggccttca
      661 gcttcttgga cgccgccatg aagtccgccc acgtctgggg ctcactggtg atcccggcct
      721 tggcgaacag gtccttgttg tagaagatgc ccgtcccgac gatgtcaccg gtgatcaggt
      781 agatcttccc gtcgggggcg cggcccgact cgatggcggg cgtgaggaac gtgtcgtacc
      841 acgaggcggc ggacgggacg taggggtcct tctgattgag caccggcgtc aggtccgcga
      901 accagttctt cttgtagtcg cgctggatcc atccgcgctg gttccagatg atgtccggcg
      961 cgtcaccgcc caccagctgc gtggtgaacc actcgtcgta gccgctcgtg atctcgggga
     1021 cgaactcgat cgtccatccg gggtgcttcg cctgccagtc cttggcagcc gtccacaggt
     1081 acgtcggcgg agccacccgc tcggactcca gcttctcccg gggcgtcagg ccctgcatgt
     1141 agacccgcag ggtcttgccc tgggcaaaga ccgtgccggc taccgcgagc accaggagaa
     1201 cgaccatcag tctcttcata gactacctcc ttgaaaacct ttccgaccgg caaactgccg
     1261 ggtggaatcc acagagtccg gcgccgtcta caccttcagg gcgcccgacg aaatgccctt
     1321 gatgaaagac ctcatcgtga agaagaacag gatgaccagg ggcgcggaag cgatcacgta
     1381 ccccgcgaac atgacccccc gggtctcgaa gctgccggtg cgctgagcga agctcgcgat
     1441 gcctacgctg atggtccaaa ggaaggtctt ggagatcgtc accaggggcc agacgtagtc
     1501 gttccaggtg cccaggatgt tcaggatggc cacggtggag aggaccggga tgctcatggg
     1561 gatggcaatg tggcggtagg cgccgacggt agagagtcca tcgatggaac ccgcgtcgaa
     1621 cagctcgttc gggatggcct ccatgaagct gcgcaggatg aagatggcga acacctgccc
     1681 tccggcaatg tagggcagga tcagcgccca gcgcgtgttc agcaggcgca gactcttcac
     1741 catgaggaac tgcggcacca ggagcaggat ggaggggatc atcaggaggc agatgatcag
     1801 gatgaagagg aactcacggc cggggaaatc gaaccgggcg aaaacgtagg ccgcaaggct
     1861 ggagacgaag agcacgccca ccaccgtcgc gctggtcacg atgaggctgt tgaggaggta
     1921 cgggtacagc cgggtccacg cgtcccggta gttcgacagc tccgcgctcg tgggcaatcc
     1981 ccagaagttg tgggagatct gataggaggt cttgaacgag gtcaggatgg tgaacacaaa
     2041 gggatagaag gtgatgatcc cgaacaggac caggaagaag tggatcggaa gatcgtgaag
     2101 ggaagctctt ctcacggcgc tcatcctccc atggcgtcgg tgttcgactt catgtacttc
     2161 aggttgatgt aggtgaggat gaggatgacc acgaagagca gggtcccgat ggagcaggca
     2221 taccccatct ttccgtactg catcgcgttg cgatacaggt acagccccgg aaccatggtg
     2281 gccttgccgg gtcccccgtt ggtcatgatc aggatgctca cgaagctctg gatggcgttg
     2341 atcatcccga ggatcaggat cagcttgacc tgggacgcga tgaggggaag ctcgatgctg
     2401 aagaaccggc gcagcgagcc ggtcccgtcg agcgtcgccg cttcggtgat ggacaccgag
     2461 atgttctgca atccggcgag atagatcagc gtctggaagc cggacaccca cgggaacccg
     2521 acgaagagga tggcgtagag cgcggtactg tattccccca gccaggtgcg ggtcagcccc
     2581 ttgaggccca tgccgttgaa cagcatgttg agaatcccgt ccgtggggct gtagatgaac
     2641 ttccagatga ggaagatgac gacggacggc accaccaggg ggatgacgta aaggaccttg
     2701 tagacgtagg ccgcccggtc cgcccgcagc cgatagatga gcaccgccgc caggagcgga
     2761 aaggtgatgg agatcaggat gctcacgatg gtgatcttga ggacgttgac caggctgaag
     2821 aggaagtcgt cgttcgaaaa caggtcggtg aagttggcaa acccgttgaa gagctggacg
     2881 ttggccccgt tccagttgaa gaaggagtgg tagatcgccg aggcggccgg atagtagttg
     2941 aagatgccga gcagggcgaa ggtgggcacc agcaggagat agcactcccg tcgcttcacc
     3001 atgcgtcgcc aaacggagtc tttgtgagcc aacggagagg aagccgcgat gctcgacata
     3061 ccggggtccc cttgtgaaac ggtcagggca ggattgtccg ggatgctcga gcgcccttcg
     3121 gacccacggc ccgccggcgc cagcatctgg ctccccggta ccacaggaac cgggcgctgt
     3181 caagcaaaat agtggtagcg cggatcggcg tgcgcgggta ccgcgcgcga gttgaccgcg
     3241 ggagccttcg accgtatctt tcaggtcgaa gaatcccttc aggagaatgc cgtgcgatac
     3301 gacaagttga ccgtcaaggc gcaggaggcc ttgcaggagg ccgactccct tgcccatact
     3361 ttcagtcacc ccgcgctgga ggtggagcac ctccttctgg ccctggtgga gcagaaggac
     3421 ggcgtggtcc cgccgatcct ggagcgcatc ggcgcggatg cgtccgaggt gtcctccgcc
     3481 atccgccacg cgctgg
//
LOCUS       DASRZS010000052         3565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5419048, whole genome shotgun sequence.
ACCESSION   DASRZS010000052 DASRZS010000000
VERSION     DASRZS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5419048"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VFH83_01245"
     CDS             <1..837
                     /locus_tag="VFH83_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6485011.1"
                     /translation="LGILKARDGDAELAIEIFRALQGLFPDDAIIRMNLALAYEERAR
                     QYEKREDEERVEEYHGLAFDAYKAALVADPSEPAIHYNIAHFYLHQRSFEKAKEHLEI
                     FVRHGDNEKRVKESRRIIREIDTQGLLDSLFKRSFDAIRMGKEEEGIQHIHEFLEKRP
                     DVPNGWFLLGWGLRRLGRYEEGKQAFLKALEIGAPHADLLNELAICLMELGDLPEAGK
                     RLREALRLEPENTKVISNLGIVALKAGNTMEALGFFRTVAELDPGDQIARRYISQLTE
                     DS"
     gene            1098..1958
                     /locus_tag="VFH83_01250"
     CDS             1098..1958
                     /locus_tag="VFH83_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HET6485012.1"
                     /translation="MIINHNMSAIFADRQLKLNGEAVQKSMEKLSSGTRITRAGDDAS
                     GLAVSEKMRSQIRGLRQASTNASNGISFIQTTEGYLQESQDIIQRLRELAVQSANGIY
                     TDEDRMQIQVEVSALVDEIDRIASHAQFNGMNMLTGRFSKPTGENTVTASMWLQIGAN
                     MDQRTRVFIGTMTSSGLGVRNIGSKEIISMSTPDGANRSIGTLDSALRTINKQRADLG
                     AYQNRLEYAVKGLDVGAENLQAAESRIRDADMASEMVEYTKNAILNQAATAMLAQANA
                     LPRSVLQLLQ"
     gene            2228..3094
                     /locus_tag="VFH83_01255"
     CDS             2228..3094
                     /locus_tag="VFH83_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HET6485013.1"
                     /translation="MIINHNMSALFANRQLKFTELDVQKDIEKLSSGQRINHAGDDAS
                     GLAVSEKLRAQIRGLNQAGRNIENAVSFVQTTEGYLGETTDIMQRIRELAVQSANGIY
                     TDEDRMQIQVEVSQLVDEVNRIASHAQFNGLNLLTGAFARPAAGANAARELFIQVGAN
                     MDQRKQVFIGTMTARALGLQGIGGTTNATISISTPDQANQVIGTVDQALQRVSRQRAD
                     LGAYQNRFEMAAKGVAVAAENLQAAESQIRDTDIASQMVDYVKNQILVQSGTAMLAQA
                     NLLPRSVLQLLA"
     gene            3322..>3565
                     /locus_tag="VFH83_01260"
     CDS             3322..>3565
                     /locus_tag="VFH83_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HET6485014.1"
                     /translation="MTDVFVKEARPVTDRPPRQQAQPELSRQEIEGQAKALEKTFQAF
                     NHRVKLTVNDSINQVVIKVLDAETDKVIKEIPAEEIQ"
BASE COUNT          756 a         1102 c         1162 g          545 t
ORIGIN      
        1 ctcgggatcc tcaaggcccg cgacggggac gccgagctgg ccatcgagat cttccgcgcc
       61 ctccaggggc ttttccccga cgatgccatc atccggatga acctcgccct ggcctacgaa
      121 gagcgggcgc gccagtacga aaagcgggag gacgaggagc gggtcgagga gtatcacggg
      181 ctggcgttcg acgcctacaa ggccgcgctg gtggcggacc cgtccgagcc ggccatccac
      241 tacaacatcg cccatttcta cctgcaccag cgctccttcg agaaggccaa ggagcacctg
      301 gagatctttg tccgccacgg ggacaacgag aagcgggtca aggaatcccg gcggatcatc
      361 cgggagatcg acacccaggg cctcctggac agcctcttca agcgctcctt tgacgccatc
      421 cgcatgggga aggaagagga aggcatccag cacatccacg agttcctgga gaaacgtccg
      481 gacgttccca acggctggtt cctcctgggt tggggattgc gccgtttggg tcgctacgag
      541 gaaggcaagc aggcctttct caaggcgctg gagatcgggg ccccccacgc cgacctgctc
      601 aacgagctgg caatctgtct catggagctg ggggaccttc cggaggcggg caagaggctt
      661 cgggaagccc ttcggctgga gccggagaac accaaggtga tttccaacct ggggatcgtg
      721 gcgctcaagg cggggaacac gatggaagcc ctgggattct tccgcacggt ggcggagctg
      781 gatccggggg accagatcgc ccggcgctac atctcccagc tcaccgaaga ttcctgaaaa
      841 atacccctaa tcttttaact cccccctgtc gagaaaagga ctgagggtag ccctggccaa
      901 aacccagtgg cggactcgtt tcttccctta ctctgcctat cggcgcacct ggagcgcccc
      961 tcaagcagga tcgggaaggg cgcgcccgtc tcgaggcgga ggtcgggggc ctcggggaac
     1021 cgtgccgggg cacgggaatc ttgggtcgtc gcgggcacgg aagcccgcgg cacaccgcag
     1081 gtttcaggga ggaaaccatg atcatcaatc acaacatgag cgcgatcttc gcggaccgcc
     1141 agctcaagct gaacggcgaa gccgtccaga agagcatgga gaagctctcc tcgggcacga
     1201 ggatcacgcg cgcgggggat gacgcctccg gtctggccgt ttcggagaag atgaggagcc
     1261 agatccgcgg gctgcggcag gccagcacga acgcgtcgaa cggcatctcg ttcatccaga
     1321 caacggaagg ctacctgcag gaaagccagg acatcatcca gcgcctgcgc gagctggccg
     1381 tccagtccgc gaacggcatc tacaccgacg aggaccggat gcagatccag gtggaagtct
     1441 ccgccctcgt cgacgagatc gaccgcatcg cgtcccacgc ccagttcaac ggcatgaaca
     1501 tgctgacggg ccgcttctcc aagcccacgg gggagaacac ggtcaccgcg tccatgtggc
     1561 tgcagatcgg agccaacatg gaccagcgca cccgggtctt catcggcacc atgacctcct
     1621 ccggcctcgg tgtccgaaac atcgggagca aggagatcat ctccatgtcg accccggacg
     1681 gcgccaaccg gtccatcggg accctggaca gcgcgctgcg caccatcaac aagcagcgtg
     1741 cggatctggg cgcctaccag aaccgcctgg agtacgcggt aaaaggcctg gacgtcggag
     1801 cggagaacct gcaggcggcc gagtcgcgca tccgcgacgc ggacatggct tcggagatgg
     1861 tggagtacac gaagaacgcc atcctgaacc aggcggccac ggcgatgctg gcgcaggcca
     1921 acgcgctgcc gcggtccgtg ctgcagctcc tccagtaagc gcgcggaacg gagcgatcgg
     1981 atcgtctgag cttggcatag accgtttcgc tctttgacag gtggaccctc ctttgggggg
     2041 tgttgggacg aggaagtcag gtccgacggg ccgccggccc cagggccggc ttccttgttg
     2101 atcggggaaa acgggaacgg tggggcgtgc gcgcgcgagc gccgcccatg acaagacccc
     2161 gcaacacaca cccgggtagg caatgccgaa ggcagggatg ccttcggagg aagtcaagga
     2221 gggtcctatg atcataaacc acaatatgag cgctctgttc gcgaacagac agctcaagtt
     2281 caccgagctg gatgtgcaga aggacatcga gaagctgtcg agcgggcagc ggatcaacca
     2341 cgcgggcgac gacgcgtccg gactggccgt gtcggagaag ctgcgggcgc agatccgagg
     2401 cctcaatcag gcaggaagga acatcgagaa cgcggtgtcc tttgtccaga ccaccgaggg
     2461 ctacctcgga gagaccacgg acatcatgca acggattcgg gaactggcgg tacagtccgc
     2521 caacgggatc tacacggatg aggatcgaat gcagatccag gtggaagtct cgcagctcgt
     2581 ggacgaggtc aaccggatcg cgtcccacgc gcagttcaac gggctgaatc tcctcaccgg
     2641 ggcgtttgcc cggccagcgg ccggcgccaa cgccgcgcgg gagctcttca tccaggttgg
     2701 agcgaacatg gatcaacgca agcaggtctt catcggaacc atgaccgcgc gcgccctggg
     2761 cttgcagggg atcgggggta cgaccaacgc taccatctcg atctccacgc cggaccaggc
     2821 aaaccaggtc atcgggacgg tggatcaggc tctgcaacgg gtttcgcggc aacgcgccga
     2881 cctgggcgcc taccagaacc gcttcgagat ggcggccaag ggagtcgcgg tagcggcgga
     2941 aaacctgcag gcggccgaat cccagattcg cgacacagac attgcctcac agatggtcga
     3001 ctatgtgaag aaccagattc tcgtgcagtc gggcacggcg atgctcgctc aggccaacct
     3061 gttgccaagg tcggtcctgc agctcctcgc ctaagcagcc aggctgccgg gacgggaacc
     3121 gttcccaacg ggagaggctt ctggacgtcg cctctttcgg atcgcagatg gggctcggga
     3181 ggtcgcgcac tccctacccc ttttcttcgc attgggatcg cagggatgaa cctccggtgc
     3241 tatccagggg ctccggaagc ggaagtacag ggaggtactg ccatgggtat ggagataccg
     3301 gggattctga aaacgaaccc cgtgaccgac gtgttcgtca aggaagctcg gccggtgacg
     3361 gatcgaccgc cgcggcagca ggcccagccc gagctgtcgc ggcaggagat cgagggccag
     3421 gccaaagcgc tggagaagac cttccaggcg ttcaaccacc gggtcaagct aaccgtcaat
     3481 gactcgatca accaggttgt catcaaggtg cttgacgcgg aaaccgataa agttataaag
     3541 gagatccccg ccgaggagat ccagc
//
LOCUS       DASRZS010000053         9486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2340536, whole genome shotgun sequence.
ACCESSION   DASRZS010000053 DASRZS010000000
VERSION     DASRZS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9486
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340536"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VFH83_01265"
     CDS             complement(<1..780)
                     /locus_tag="VFH83_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485015.1"
                     /translation="MRRSGRTSLLALGDRYRIYIILVVVFVVMSLFAPKFFNARNFTT
                     ILNAVAMNATVAIGFTVVMICGQLDLSIGSVITFAGVLTMGLEPTLGFAGSIAVAVAG
                     GALVGLVNGLLVTKARINSFIVTLGTMTVLQGSIYQFSGGNSISVSTDQAFAISDFLG
                     KNLLPLVTPRILITLILLVVVEFVLRRTKAGRNLYITGGSRTTAWLAGINTDRYLIAA
                     FVLSGVTAAIGGIVSDLVSTAATLNLGENSLMYVISATIIGG"
     gene            complement(773..1831)
                     /locus_tag="VFH83_01270"
     CDS             complement(773..1831)
                     /locus_tag="VFH83_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485016.1"
                     /translation="MSAEPSPSASAPRAAVSDTRSIRAGSFSSFMNTAGVYAIALLFL
                     ALGALLQATGAINGFLSPKNLLNIVDAVALVGIVAVGMSFVTYSGNYADMSVPTTMGF
                     TGYICIELLRFNFGLAIAGALVVGILIGALNGFVVGRFKANPIIWTLAVNYVTLGVIR
                     LVWVNKQIYPDMVGSPQHAVNLFDAIYRLRFFNLIGLPLVMLIVLVIVGQFVLTRTSF
                     GVKLKITGSSPRAAKLSGIAVERAILLSFVLVAFTATVAGLAVTSLSRVGAWYNGAGY
                     DFKAVTAVVIGGMTLAGGRGTILGVLGGALIIGILNNIMTLIGIGTFSQDIIRGAIFI
                     IVVGINALSLRRMGRDDA"
     gene            complement(1828..3351)
                     /locus_tag="VFH83_01275"
     CDS             complement(1828..3351)
                     /locus_tag="VFH83_01275"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6485017.1"
                     /translation="MSEPASGYALEMKRVGKRFPGTIAVDDVTFEVRPGEVHALVGEN
                     GAGKSTLMKILAGSFDDYTGEIAINGHVALLHSPTLAKRHGIGMIYQELSLARPISIT
                     ENLLAGRLPRRGPFLDKARARTQARELLAEVGLDLDPSLPIEDISQHEAQLVEIAKVL
                     GNRPSILVMDEPTSALSREEVERLFTIIGELKGRGIAIVYISHHLPEIFRIADRVTVM
                     RDGKKIATRPVGEVSREEIVQMMVGRSVKEFYRRGERSIGEVRLRVRGLSRFGFFHGI
                     DLEARRSEILGICGLSGAGRTELARVLTGLDRSDEGTIELDGQRVRGSSMADFLDQGI
                     SYLTEDRKTEGLAVRLAVDDNILSAIVDRLSSASVFSKARGKPAVERLIAELSIYPPD
                     PSRTVANLSGGNQQKVLLAKWLATQPKVLILDEPTRGVDIGAKMTIHASIEKLAREGC
                     TVLLISSDLPELVGLCDRVMIMRKGHFIREMPYSECSEESLLLAANGDTGHGEGRAS"
     gene            complement(3430..5172)
                     /locus_tag="VFH83_01280"
     CDS             complement(3430..5172)
                     /locus_tag="VFH83_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6485018.1"
                     /translation="MFSSNDRLLISWNTRPKEIPPRPRNPLQFPQTDARHWYDQEYAG
                     WNVQKCGIPDSPGDGSRGKRVAFLRPGLHPYYVAFAEGLERIAERAGVIVRTLTGTMT
                     MESQEELVHSVLAEPPDLLILVPISSEACTRWVQRLHAGGVPVIVSNFIPNDEAYRYV
                     LAWCGPDDWGQYRMLARSFADRLGRRGGYAIVRHIPGTSCYDARCWSVVTELAVVAPQ
                     MRCLAMGEASREGTFDPERADALVTGWLAELGGELRGIVGPDDDILVDGINEALRRAG
                     REDVVRVGAGSTQKGMALVKAGRLHAITFQPAQADGALAMKIAVDWFNGLDIQPINYL
                     PKHIITRENVDDFISRKPEFPAVSLEGLTRAIVEGSERQVERFFEDAYQSLLSAELIT
                     PEFFRGFTIEVLSEVIHLLKMNDLDGRALLSDSESLYKNLFNQKTPRRALEWLMGTAK
                     LAIRELAGARQAETPIARIVRYVERNYAEPLSLKVLAVQFGLSAPYLGRLFHTETGRS
                     FTTYLNELRLRKAEELLRYSNLKSSEIAARTGYSNVNYFYTLFRKYRGYYPSESKARA
                     CGDEEAPVHSLKRK"
     gene            5257..6051
                     /locus_tag="VFH83_01285"
     CDS             5257..6051
                     /locus_tag="VFH83_01285"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HET6485019.1"
                     /translation="MSLFRGLRRTHIIKESLLPEDVWAWLRDDHPILNGLSQKELEQL
                     RRLSTLFLHEKRFEGADGLSLTDGMREVISVQACLPILNLGMDWYENWKTVVVVPDVF
                     VEEHTAFDDAGVAHEWDEDQSGESWDSGPVLLSWKDVEASGWGEGYNVVIHEAVHRLD
                     LLDGAANGRPSLHEGMDPEEWRTVFSSAYRDLKGRTGRGHKRSRIDSYAVEDDAEFFA
                     VTSEYFFEQPRVLRSEYPEVYRLESAFFRQDPAARDRGKGDPQRRS"
     gene            complement(6036..7358)
                     /locus_tag="VFH83_01290"
     CDS             complement(6036..7358)
                     /locus_tag="VFH83_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6485020.1"
                     /translation="MASKTHSSAGLALTGALDRLVEPPLRRNFLAFLGDYVIWGVGIA
                     FVNPSTVLPALVRQLTDSPPVIGLVETVRSGVWFLPQLFVAWLLSGTVIRRRHMVIPF
                     IISRSSMLLIVVVLLLLARTQPLTAAGLLLACLAFYFIMDASGSVPWFDLLSHSLPPH
                     GRTRLMGLGQFLSGGLGIGVGVAVAAILADTRLPFPVNYSLLFILACSLFAVEVFVVS
                     RVRVSRPADRRDRIPLAEFVPRLLGIFRRDLRFRRLLLVRLVVGASGLSTPFYIICAL
                     DRLGMGAASVGIFTAAQIAGGIAGAPFIALLAERRGVTSVIRIGASFALILPIIAISM
                     VTAGLSLSHDLRIAVFGLLFVLNGAMANMTAAGFTNYMLDIAPAEQRPTYVGFANTFN
                     GLILFFPIVGGWILGDSSWVALFAVTAAAALVGLVGTLRMSEPRQLRR"
     gene            complement(7439..8980)
                     /locus_tag="VFH83_01295"
     CDS             complement(7439..8980)
                     /locus_tag="VFH83_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET6485021.1"
                     /translation="MIRTIDALLNRLTMYRLTLYYLMALLGLGLALSFFHLVSPAPLA
                     ILSTTAVLLAACYAANWAAARLLRIPSNPESSLITALILALIVGPVSFMEDPGRVGLL
                     ALAGVASVASKYILAFRRQHAFNPAAVGALVAGVVLGLDVTWWVGSLPMLPLVAIGGL
                     LLAWRVHRMRFVAVFLGAFLVFLLGMGLAQGLTPDLALTTLKLVFGQSALVFFAVVML
                     TEPMTSPKRFPLQILYAALVAFLYQPQLSVLGQNLTPEEALLVGNLFSFLVSPSRKLK
                     LALKERREIGKGIMSFSFPQPAGFRHRLGQYMEWTLPIKNGDSRGSRRYFSIASSPTE
                     PELLIAARFYPRSSRYKEELARMGTGAVVLAGELGGDFVLPRDPMKPLVFIAGGIGIT
                     PFRSMLKYLVDTGQRRDIVLLYSNNTEEEIVFRDVLCDAERATGLKVVYTLTDQDKVP
                     PDWPGRIGLIDAGMLREEVPGLASREVFVSGSPGMVRAMTGVLRSAGVARANLRTDAF
                     SGYSG"
     gene            complement(8977..>9486)
                     /locus_tag="VFH83_01300"
     CDS             complement(8977..>9486)
                     /locus_tag="VFH83_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET6485022.1"
                     /translation="QLTNGFFDVRFQGRFDPSGLVKGWAIQKAADLLEGDGFVSFCIE
                     AGGDIEVRGANEQGKPWVIGVRSPFETSKIIRTVSLQNRGIATSGTYIRGEHIYNPLT
                     GARAAEIASLTVVGPNVYEADRIATAAFAMGRAGAQFVSSLSDLDCYLIEWSGSAAYT
                     PGFARYLTS"
BASE COUNT         1803 a         3028 c         3171 g         1484 t
ORIGIN      
        1 tccgccgatg atcgtcgccg agatgacgta catcagcgag ttctcgccga ggttgagcgt
       61 tgccgcggta gagaccaggt ccgacacgat gccgccgatg gcagccgtga cgccggagag
      121 cacaaacgcc gcgatgaggt agcggtccgt gttgatgccg gcgagccagg ccgtggtccg
      181 gctgcccccg gtgatataga ggttgcgccc ggccttggtg cgccgcagga cgaactccac
      241 gaccaccaga agaatcaggg tgatcaggat ccgcggcgtg accagcggga gcaggttctt
      301 gcccaggaag tcggagatgg cgaaggcctg gtccgtcgac acgctgatcg agttgccgcc
      361 ggagaactgg tagatggagc cctggagcac ggtcatggtg cccagggtga cgatgaacga
      421 gttgatgcgg gccttggtga ccaggagccc gttcaccagc cccaccagcg ctccgccggc
      481 caccgccacc gcgatggaac ccgcgaagcc cagcgtaggc tccagcccca tggtaaggac
      541 accggcaaag gtgatcaccg acccgatgga gaggtcgagc tggccgcaga tcatcaccac
      601 cgtgaagccg atggccacgg tggcgttcat ggccaccgcg ttcaggatgg tggtgaagtt
      661 gcgggcgttg aagaacttcg gcgcgaagag ggacattacc acgaacacga cgaccaggat
      721 gatgtagatc cggtagcggt cccccagggc gagcaggctg gttcttcccg acctacgcat
      781 catccctccc catgcgtcgg agcgacaggg cgttgatgcc gaccacgatg atgaagatgg
      841 ccccccgaat gatgtcctgg gagaaggtcc cgattccgat gagcgtcatg atgttgttca
      901 ggatcccgat gatcagcgct ccgccgagca cccccaggat ggtgccgcgt cctccggcca
      961 gggtcatgcc gccgatgacc acggccgtca cggccttgaa gtcgtaaccg gctccgttgt
     1021 accaggcgcc gacgcgggaa agggaggtca cggcaagacc cgccacggtg gccgtgaagg
     1081 ccaccagcac gaaggagagc agaatggccc gctccaccgc gatgccggag agcttggccg
     1141 cgcgcgggga agagccggtg atcttcagct tcacgccgaa ggacgtccga gtcagcacga
     1201 actggcccac gatcaccagg acgatgagca tgaccagggg cagcccgatg aggttgaaga
     1261 accgaagccg gtagatggca tcgaagaggt tcaccgcgtg ctgcggagag ccgaccatgt
     1321 ccggatagat ctgcttgttg acccacacca ggcggatcac gcccagggtc acgtagttca
     1381 cggccagggt ccagatgatg gggttggcct tgaacctgcc gaccacgaaa ccgttcaggg
     1441 cgccgatcag gatcccgacc accagggcgc cggcgatggc aagaccgaag ttgaaccgaa
     1501 gcagctcgat gcagatatag ccggtgaacc ccatcgtggt tggaaccgac atgtcggcgt
     1561 agttgccgga gtacgtgaca aaggacatgc cgaccgcgac gatcccgacc agggccaccg
     1621 cgtccacgat gttgaggagg ttcttgggtg acaggaatcc gttgatggcg cccgtggcct
     1681 gcagaagggc gcccagcgcc aggaacagca gggcgattgc atagacgccc gccgtgttca
     1741 tgaagctgga gaacgagccg gcccggatgg accgggtatc cgagacggcc gcgcgcggag
     1801 cgctggcgga cgggctgggc tccgcgctca tgacgccctt ccctcgccgt ggccggtgtc
     1861 accgttggcg gcaaggagga ggctctcctc gctgcactcg ctgtagggca tctcccggat
     1921 gaaatgtcct ttccgcatga tcatcacccg atcgcacaga ccgaccagct cagggaggtc
     1981 cgacgaaatc aggaggacgg tgcacccctc ccgcgccagc ttctcgatgg aggcgtggat
     2041 cgtcatcttg gcgccgatat cgactccgcg cgtgggctcg tccaggatga ggaccttcgg
     2101 ctgcgtagcc agccacttgg ccagcagcac cttctgctga ttgccgccgg agaggttggc
     2161 cacggtccgg gaggggtccg gcgggtagat ggagagctcg gcgatcagcc tctctaccgc
     2221 aggcttgcct ctggctttgg agaagacgga cgccgaggag aggcgatcca cgatggccga
     2281 gaggatgttg tcatccaccg cgagacggac cgccaggcct tcggtcttgc ggtcctccgt
     2341 caggtacgag attccctggt ccaggaagtc cgccatggag gagccgcgca cccgttgccc
     2401 gtccagctcg atggttccct catccgagcg gtccagtccg gtgagcacgc gggccagctc
     2461 ggtccgcccg gcgccggaca gaccgcagat gccgaggatc tcggacctgc gggcttccag
     2521 gtcgatcccg tggaagaatc cgaaccgcga aagccctctc acgcggagac ggacctcgcc
     2581 gatggatcgc tctccgcgcc ggtagaactc cttcacggat cgaccgacca tcatctgcac
     2641 gatctcctcc cggctcacct cgccgaccgg ccgggtggcg atcttctttc cgtcccgcat
     2701 gaccgtgacg cggtccgcga tccggaagat ctcgggaagg tggtgggata tgtacacgat
     2761 ggcgatgccc cggcccttca gctccccaat gatggtgaac agccgctcga cctcctcccg
     2821 ggagagggcg ctcgtcggct cgtccatgac gagaatggac ggtcggttcc ccagaacctt
     2881 ggcgatctcc acgagctgcg cctcgtgctg gctgatgtcc tcgatgggca ggctcggatc
     2941 caggtccagg cccacttccg ccagcagctc ccgggcctga gtccgtgccc gtgccttgtc
     3001 caggaacggc ccccgccggg gcaggcggcc ggccaggagg ttctccgtga tgctgatggg
     3061 gcgcgccagg ctcagctcct ggtagatcat gccgatgccg tggcgcttgg cgagcgtcgg
     3121 agagtgcagc agggcaacgt gtccgttgat cgcgatctct ccggtgtagt cgtcgaacga
     3181 gccggccagg atcttcatca gggtcgactt gccggcgccg ttctcgccga ccagggcgtg
     3241 gacctctccg ggccgcacct cgaaggtcac gtcatccacg gcgatggttc cggggaagcg
     3301 cttgccgacg cgcttcatct ccagcgcgta cccggatgcc ggttcgctca ctcttcacgc
     3361 tccttggcgg attgggattt gctgtggcat cctacgcccg cacgcggggc atcgcaacac
     3421 cagaaacttt cactttcgct tcaaagaatg caccggggct tcctcgtcgc cgcaggcgcg
     3481 ggccttggac tccgagggat agtagccgcg gtacttgcgg aagagcgtgt agaaatagtt
     3541 cacgttggag taaccggtcc gggcggcgat ctcgctcgac ttgaggttgc tgtagcgcag
     3601 gagctcttcc gcctttcgca gccgcagctc gttcaggtag gtggtgaagg atctgcccgt
     3661 ctcggtgtga aagagcctcc ccaggtacgg cgcggagaga ccgaactgga cggccagcac
     3721 cttcagggac aacggctccg cgtagttgcg ctcgacgtac cgaacgatcc gagcgatcgg
     3781 cgtctcagcc tggcgcgccc ccgccagctc gcggatggcg agcttggcgg ttcccatcag
     3841 ccactccagc gcccgtctcg gggtcttctg gttgaagagg ttcttgtaga ggctctcgga
     3901 atcggagagg agcgcccgac cgtcaagatc gttcattttc aggagatgga tgacctcgga
     3961 gagcacctcg atggtgaacc ctcggaagaa ctccggcgtg atcagctccg cggaaagcag
     4021 gctctgatag gcatcctcga agaaacgctc gacctgccgc tcgctgccct ccacgatggc
     4081 gcgcgtcaac ccttccagag aaacggcggg gaactccggc ttgcgggaga tgaagtcatc
     4141 cacgttttcc cgcgtgatga tgtgcttcgg caggtagttg atgggctgga tgtcgagccc
     4201 gttgaaccag tccacggcga tcttcatcgc cagggccccg tccgcctgcg ccggctggaa
     4261 cgtgatggca tggagccggc ccgccttgac cagtgccatg cccttctggg tcgagcccgc
     4321 gcccaccctg acgacgtctt ccctccccgc gcggcggagg gcctcgttga tcccatccac
     4381 caggatgtcg tcgtccggcc ccacgatccc gcgcagctcc ccccccagct ccgcaagcca
     4441 tccggttacc aaggcatcgg cccgctcggg atcgaaggta ccctcgcggc tcgcctcccc
     4501 catcgccaga cagcgcatct ggggcgcgac gacggccagc tcggtgacga cggaccagca
     4561 gcgcgcatcg tagcaggagg taccggggat gtggcggacg atggcgtatc ccccgcgccg
     4621 tcccaatcgg tcggcaaagc tccgagccag catccggtac tggccccagt cgtcggggcc
     4681 gcaccaggcc aggacatacc gataggcctc gtcgttgggg atgaagttgc tcacgatgac
     4741 gggcacgccg ccggcatgca gccgctggac ccaccgggta cacgcctcgg agctgatggg
     4801 caccaggatc aggaggtccg ggggttccgc aaggacgctg tggaccagct cctcctggct
     4861 ctccatcgtc atcgtgcccg tgagcgtgcg gacgatgact cccgcccgtt ccgcgattct
     4921 ctcgagacct tccgcgaagg cgacgtagta cgggtggagc ccgggccgca gaaaggcaac
     4981 cctctttcct cgcgatccat cgccggggga atcggggatc ccgcacttct gcacgttcca
     5041 gcccgcgtac tcctggtcgt accagtgtcg ggcatcggtc tgcgggaact ggagcgggtt
     5101 gcggggccgt ggggggatct ccttgggcct cgtgttccac gatatgagga gccgatcgtt
     5161 gctggagaac atcgcctggc gcccatgata gcggaacaca accgggctgc caaccgattg
     5221 accgagtcag cggcgccgac cgacattgga ggcgagatga gcctgtttcg cggactccga
     5281 cgcacccaca tcatcaagga gagcctcctg cccgaggacg tctgggcatg gctgcgggac
     5341 gatcatccga ttctgaacgg gctttcccag aaggagctcg agcagcttcg acggctatcg
     5401 acgctctttc tccatgagaa gcggttcgaa ggcgccgacg gcctttctct tacggacggg
     5461 atgcgggagg tgatctcggt ccaggcgtgc ctgcccatcc tcaacctcgg catggactgg
     5521 tacgagaact ggaaaacggt cgtcgtggtg cccgacgtgt tcgtggagga gcacaccgcg
     5581 ttcgacgatg cgggggtagc ccacgagtgg gacgaggacc agagcgggga gtcctgggac
     5641 agcggcccgg tacttctatc gtggaaagac gtcgaagcgt cgggatgggg cgaggggtac
     5701 aacgtggtca tccacgaggc ggtccaccgc ctggacctgc tggatggcgc tgccaacggt
     5761 cggccatccc tccacgaggg aatggatccc gaggaatggc gaacggtgtt ctcctccgcg
     5821 taccgggacc tcaagggccg gaccggccgc gggcacaagc gctcgcggat cgattcctac
     5881 gccgtggaag acgacgcgga gttcttcgcg gtgaccagcg agtacttctt cgagcagccc
     5941 cgcgttctgc ggtccgagta cccggaggtc taccgcctgg agagcgcgtt cttccgtcag
     6001 gacccggcgg cccgggatcg cggcaagggc gatcctcagc ggcggagctg acgcggctcg
     6061 gacattcgaa gggtcccgac caggccgaca agcgccgcgg cggcagtcac ggcgaacaga
     6121 gcgacccagg acgaatcccc gaggatccat cctccgacga tcgggaagaa caggatgagc
     6181 ccgttgaagg tgttcgcaaa tccgacatag gtgggacgct gctccgcagg cgcaatgtcc
     6241 agcatgtagt tggtgaaccc ggcggccgtc atgttcgcca tggcgccgtt caggacgaag
     6301 agcagcccga acaccgcgat ccgcaggtcg tgggacaggc tcagccccgc ggtgaccatg
     6361 ctgattgcga tgatgggcag gatcagcgcg aacgatgccc cgatccggat gaccgaagtg
     6421 accccgcgcc gctccgccag gagggcgatg aacggcgcac cggcgatccc acccgcgatc
     6481 tgggcagcgg tgaagatccc tacgctggcg gcccccatcc ccagtcggtc cagggcgcag
     6541 atgatgtaga acggcgtgga cagacccgag gcgcccacca ccagccggac gaggaggagc
     6601 cggcggaagc ggagatcgcg acggaagatg cccaggagcc gggggacgaa ctctgccagg
     6661 gggatccgat cccttcggtc cgccggccgc gagacccgga ctcgggatac gacaaagacc
     6721 tccaccgcga agagcgagca ggccagaatg aagagcaagg agtagttgac ggggaacggg
     6781 agacgggtgt ccgccaggat cgccgccacg gcaacgccca ccccgatccc cagaccaccg
     6841 gagaggaact gccccaaccc catcaggcgg gtccggccat gcggaggcag ggaatgcgac
     6901 agcaggtcga accaggggac cgagccgctg gcatccatga tgaaatagaa ggccagacag
     6961 gcaaggagca gaccggccgc ggtcaggggc tgcgttcggg ccaggaggag aagcaccacc
     7021 acgatgagga gcatcgagga tcggctgatg atgaacggga tcaccatgtg ccggcgccgg
     7081 atcaccgtgc cggaaaggag ccaggccacg aagagctgcg gcagaaacca cacgccgctg
     7141 cgaacggtct ccaccagacc gatcacgggc ggagaatccg tgagctgtcg gaccagggcg
     7201 ggcagcaccg tgctgggatt cacgaacgcg atcccgacgc cccagatcac gtagtccccg
     7261 agaaaggcaa ggaagttgcg tcgaaggggc ggttccacca ggcggtccag cgcgcccgtc
     7321 agggcgagcc ccgccgagga atgggtcttg gatgccacgg tcgtgcgggt ataggacaaa
     7381 cgggaggctt tggcaagcct cccgtgtcgg ccgagcgggc ggtccagcgc aggcgacgtc
     7441 agccggagta gccggagaag gcgtccgttc ggaggtttgc tcgagccacg ccggcggaac
     7501 gcaggacgcc cgtcatcgcc cgcaccatcc cgggagaacc ggagacgaat acctcccggc
     7561 tcgccaggcc agggacctct tcccgaagca tccccgcgtc gataaggccg atccggcccg
     7621 gccagtcggg cggaaccttg tcctggtccg taagcgtata gaccaccttg agacccgtgg
     7681 cgcgctccgc atcgcacaat acgtcgcgga acacgatctc ctcttcggta ttgttggaat
     7741 agagcagcac gatgtcccga cgctgtccgg tatccaccag gtacttgagc atgctccgaa
     7801 acggcgtgat gccgattccc ccggcgatga acaccagcgg tttcatcgga tctcttggca
     7861 gcacgaagtc cccgccgagc tcgccggcca gcaccacggc tccggttccc atcctggcaa
     7921 gctcttcctt gtaccggctc gacctggggt agaaccgggc cgcgatcaga agctcgggct
     7981 cggttgggga cgacgcgatg ctgaagtagc gccgactgcc gcggctgtcg ccgttcttga
     8041 tcgggagggt ccactccatg tactgcccga gacgatgacg gaacccggcc ggctggggaa
     8101 aggagaagct catgatcccc ttgccgatct cccgacgctc cttgagcgcc agcttgagct
     8161 tgcggctcgg gctgaccagg aacgagaaga ggttgccgac caggagcgcc tcctccgggg
     8221 taaggttctg tccgagcacg gatagctggg gctggtagag gaaggccacc agggccgcat
     8281 agaggatctg gagcgggaag cgtttggggg aagtcatcgg ctcggtcagc atgaccacgg
     8341 cgaagaacac cagggcgctt tgcccgaaga cgagcttcag cgtggtaagg gcgagatcgg
     8401 gggtcagtcc ctgggcaaga cccatcccaa gcaggaacac caggaacgcg ccgaggaaca
     8461 cggccacgaa tcgcatccga tggactcgcc aggccaggag caaaccgccg atcgcgacca
     8521 gcggaagcat ggggaggctt ccgacccacc aggtcacgtc cagcccgagc actacgccgg
     8581 caacgagggc cccgaccgcg gccgggttga acgcatgctg cctccggaat gccaggatgt
     8641 acttggaggc gacggacgcc actccggcca gcgccaggag cccgacccga cccgggtctt
     8701 ccatgaacga gaccggtccg acgatcagcg ccaggatcag cgctgtgatc agggaagact
     8761 ccggattcga gggaatcctc aggagccgcg ccgctgccca gttggcagcg tagcaggcag
     8821 ccagcagaac cgccgtggtg gacaggatgg cgagcggagc cggcgacacc aggtggaaga
     8881 agctcaaggc cagtccgaga cccagaagcg ccatcaggta gtagagcgtc aggcggtaca
     8941 tggtcaggcg attgagcaat gcgtcgatcg tgcggatcat gatgtcaggt acctcgcaaa
     9001 cccaggggta taggcagccg atccggacca ctcgatcagg tagcagtcca ggtccgaaag
     9061 gctcgatacg aactgcgcgc cggcgcgccc catggcaaag gctgcggtgg cgatccggtc
     9121 cgcctcgtag acgttgggcc caaccacggt gaggctggcg atctcggcgg cgcgcgcgcc
     9181 ggtgaggggg ttgtagatat gctcgcctcg gatataggtc ccggaggtgg cgatcccgcg
     9241 gttctgcagg ctgacggtcc ggatgatttt cgaggtttcg aaggggctgc gaaccccgat
     9301 gacccacggc ttgccctgct cgtttgcgcc gcgcacctcg atgtcgccgc ccgcctcgat
     9361 gcagaagctg acgaagccgt ccccctccag cagatcggcg gccttctgga tggcccagcc
     9421 cttcaccaga ccggatggat cgaatctccc ctgaaacctc acgtcgaaga aaccgttcgt
     9481 gagctg
//
LOCUS       DASRZS010000054         4464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4651920, whole genome shotgun sequence.
ACCESSION   DASRZS010000054 DASRZS010000000
VERSION     DASRZS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4651920"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="VFH83_01305"
     CDS             <1..1624
                     /locus_tag="VFH83_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6485023.1"
                     /translation="GDPKILQDALSGFDRLPPLLRQLRSLSRDPADVRDLQTISQAGE
                     DYQNACAAVIESSRLMVTLENNRSAATQVVLDAARTTSVAGLADARDVTGLSAARLLS
                     AVLILIAGLIAAAVIGIALSIAITRAITRPLAKGVALAQRVAAGDFTQLLQMRGRDEV
                     GMLVRALNGMSVKLRAMVATVRDNAEQVAAASGQISASAQRLSEGAQSQASTLEQTSA
                     SVQELSASVDLVSANAQSQVLAVEEGTASMAQVQKTIEEVSTNLTAISGLAERNVENA
                     VAGARAVQEVVTGIARIEKSSKRISGIVEVISDIADQTSLLALNASIEAARAGEHGRG
                     FAVVADEVSKLADRSAASTKEIEGLIEESVQNVAEGVQTALGSQTAMEQIRDASQNVK
                     QMIGALAASMQAQVTAIRELAASLKNVSEMSGSISAATQEQTVNARQVSRAVENVNEL
                     TQSAASAAEEMSSATEQLSTMAKGLHQMVAHYRIDADDEEEQPALDRDAEPPVPEIDA
                     DALPSEDLVEETAEDGDDVEEKDPEPSPLGLS"
     gene            1782..3092
                     /locus_tag="VFH83_01310"
     CDS             1782..3092
                     /locus_tag="VFH83_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6485024.1"
                     /translation="MKRVFPALVVLGLISTTVFAAGSQEKGGGTAPSGKIGGSVDVLA
                     TWGGSEQDIFNAMVKPFTDRTGVAINYTGTRDLDAILTTRVAAGNPPELSGLPNPGSM
                     AGFARQGKLVDLSTVLDVNAMKSAYAQSWLDLGSVDGKLVGIFTKASLKGLVWYDPKN
                     LQAAGLQVPTTFDQLMSESQTSAAKGVTPWAIGVESGSASGWVGTDWLENIFLHMYGP
                     ATYMAWYQGKLAWTSPEVKSVWQTWGKIVSDPSMVYGGSQYVMSTNFNTAFSPLFQNP
                     PQAYFHFQATFIQSFIQQQFPNLKPVEDFNFFGFPSIASQYAKAVEIAGDLFGMFKKT
                     PQASALLTYLTTAEAQDYWVKAADGISPNRTVSLSDYPDPLSKNAAQILTSADIAVFD
                     ASDMMPAKMNNAFWAAIMNYLAHPDQLDSILVGLEQVRKDAYSM"
     gene            3114..4094
                     /locus_tag="VFH83_01315"
     CDS             3114..4094
                     /locus_tag="VFH83_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015221934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485025.1"
                     /translation="MAAKIIWAVVAVLGVPMALGGYLYAAERAIRGLGPARRSAVRPW
                     VWLAPAFLLVAGLLIYPALTTISLSLRSADSARFIGLANFRFIVTDSSMLIVLRNNLL
                     WLVFFTLITITVGLLIAVLTDRVRYESAAKAVIFLPMAISFVAAGVIWKFMYQFQPAG
                     TAQVGTVNAILTAVAPRFRPQAWLVNESENNPALIIVGIWMWTGFAMVILSAGLKGIP
                     TSVLEAARMDGAGELQIFTRITLPLLAPTLTVVATTLIINVLKVFDIVYVMTNGNLNT
                     EVIANRMYKEMFNYQDFGRASAFAVILLLAIVPVMVINIRRFGRSREGMA"
     gene            4091..>4464
                     /locus_tag="VFH83_01320"
     CDS             4091..>4464
                     /locus_tag="VFH83_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485026.1"
                     /translation="MSSRVLTSARGPAMSPVARLLTRGPLHVVLVVVGLLWLLPSVGL
                     LVSSFRPSSVVSSTGWWMAFLNPLQFTLSNYGYVLGRANMALSFANSLVIAIPAIVMP
                     ILIVAFGVYVFAWMKFLGCNVL"
BASE COUNT          760 a         1488 c         1455 g          761 t
ORIGIN      
        1 gggagacccc aagatcctcc aggatgcgct cagcggattc gaccggctcc cgcccctcct
       61 gcgtcagctt cggtccctca gccgggatcc cgccgatgtc cgcgacctgc agaccatctc
      121 ccaggcggga gaggattacc agaacgcgtg cgcggccgtc atcgagagca gccgcctcat
      181 ggtgaccctg gagaacaacc gctcggccgc gacccaggtc gtgctggacg cggcgcgcac
      241 cacctccgtg gcgggtctgg ccgacgcgcg tgacgtgacg gggctcagcg ccgcgcggct
      301 cctgagcgcc gtgctcatcc tgatcgcggg tctgatcgcg gccgccgtca tcggcatcgc
      361 cctctccatc gccatcacgc gggccatcac ccggccgctg gcaaaaggtg tggccctggc
      421 ccagcgggtc gcggccggcg acttcacgca gctcctgcag atgcgggggc gggacgaggt
      481 gggcatgctg gtgcgcgcgc tgaacgggat gtccgtgaag cttcgcgcca tggtggccac
      541 ggtacgggac aacgccgagc aggtggcggc cgcaagcggt cagatctccg ccagcgcgca
      601 gcggctttcc gagggcgccc agagccaggc gtccacgctg gagcagacga gcgcctcggt
      661 ccaggagctg agcgcgtcgg tggacctggt gtccgccaac gcccagagcc aggtcctggc
      721 cgtggaagag gggactgcct cgatggccca ggtgcagaag accatcgagg aggtctcaac
      781 caacctgacc gccatctcgg ggctcgccga acgcaacgtg gagaacgcgg tggccggtgc
      841 ccgcgccgtg caggaagtgg tgaccggcat cgcccgcatc gaaaagagct ccaagcggat
      901 cagcgggatc gtggaggtca tctccgacat cgcggaccag accagcctcc tggcgctcaa
      961 cgcctccatc gaggcggctc gcgcggggga gcacggccga gggttcgcgg tagtcgccga
     1021 cgaggtgagc aagctggcgg accgaagcgc cgcctccacg aaggagatcg aaggtctcat
     1081 cgaggagagc gtccagaacg tggcggaagg cgtccagacc gccctgggct cgcagaccgc
     1141 catggagcag atacgagacg cttcgcagaa cgtcaagcag atgatcggcg ccctggcggc
     1201 gtccatgcag gcgcaggtga ccgctatccg ggagcttgcc gcctctttga agaacgtgag
     1261 tgagatgagc ggcagcatct ccgcggctac gcaggagcag acggtcaacg cgcgccaggt
     1321 gtccagggcc gtggagaacg tcaacgagct cacccagtcc gcggcgtcgg ccgcggagga
     1381 gatgtcttcc gccaccgaac agctttccac gatggccaag gggcttcacc agatggtggc
     1441 gcactaccgg atcgacgccg acgacgagga ggagcagccc gccctggacc gggacgccga
     1501 gcctcccgtt cccgagatcg acgccgacgc gctgccctcg gaggatcttg tcgaggaaac
     1561 cgccgaggac ggcgatgacg tcgaagagaa ggaccccgag cccagccccc tggggttgag
     1621 ctgagcggcc atccgagtcg ccgagggtcg cctcgccgaa gccgcaatcg gcttgccttt
     1681 ttcaacagcc gttagtaact cagtctagcg cccgtgccgc gttcgtcgcg gccatcgcgg
     1741 cccggtacgg cgcgattctc tatgcgtcag gagggacacc aatgaaaaga gtgttcccgg
     1801 ctctcgtcgt acttggcctc atctccacca ccgttttcgc agcaggttcc caggagaagg
     1861 gcggcggcac cgcgccttcc ggcaagatcg gcggctccgt cgacgtgctg gcaacctggg
     1921 gaggttcgga gcaggacatc ttcaacgcga tggtgaagcc attcacggat cgcaccggcg
     1981 tggccatcaa ctacaccggt acgcgggacc tggatgccat cctcaccacc cgggtggctg
     2041 ccggcaaccc acccgagctc tcggggcttc ccaaccccgg ctccatggcc ggcttcgcca
     2101 ggcaggggaa gctggtggac ctttcgacgg tgctggacgt gaacgccatg aagtcggcgt
     2161 acgcccagtc ctggttggac ctggggagcg tggacggcaa gctggtcggg atcttcacca
     2221 aggcctccct caagggcctg gtgtggtatg acccgaagaa cctccaggcg gccgggctcc
     2281 aggttcccac cacctttgac cagctcatga gcgagtccca gaccagcgcg gccaagggag
     2341 tgacgccctg ggccatcggc gtggagagcg gttccgccag cggatgggtg ggcacggact
     2401 ggctggagaa catcttcctc cacatgtacg gacccgccac ctacatggcc tggtaccagg
     2461 gaaagctcgc ctggacctcg cccgaggtga agtcggtctg gcagacctgg ggcaagatcg
     2521 tctcggatcc gtcgatggtc tacggcggga gccagtacgt gatgtccacg aacttcaaca
     2581 ccgccttctc accgctgttc cagaacccgc cgcaggcata cttccacttc caggcgacgt
     2641 tcatccagag cttcatccag cagcagttcc ccaacctgaa gccggtggag gacttcaact
     2701 tcttcggctt cccgtccatc gcctcgcagt acgccaaggc ggtggagatt gccggtgacc
     2761 tcttcggcat gttcaagaag acgccgcagg cctcggcgct cctgacctat ctcaccaccg
     2821 cggaggccca ggactactgg gtgaaggccg cggacggcat ctcgcccaac cgcaccgtgt
     2881 cgctttccga ctacccggat cccctcagca agaacgccgc ccagatcctc acctcggccg
     2941 acatcgcggt gttcgacgcc tccgacatga tgcccgcaaa gatgaacaac gcgttctggg
     3001 cggccatcat gaactacctg gcgcacccgg accagctgga cagcatcctc gtggggctgg
     3061 agcaggtccg taaggacgcc tactcgatgt aggcgcgccc gatcgagcag ccgatggcgg
     3121 ccaagatcat ctgggcagtg gtggccgtgc tcggtgtccc gatggcgctc ggcggatatc
     3181 tctacgccgc cgagcgcgcc atccggggcc tgggaccggc ccgacgcagc gcggtgaggc
     3241 cctgggtctg gcttgcgccg gccttcctcc tggtggcggg gctcctgatc tatccggccc
     3301 tcaccacgat atcgctgagc ctgcgaagcg ccgattcagc gcgcttcatc gggcttgcca
     3361 atttccggtt catcgtgacg gactccagca tgctgatcgt gctgaggaac aacctgctct
     3421 ggctggtgtt cttcacgctc atcacgatca ccgtggggct cctcatagcc gttcttaccg
     3481 accgcgtccg ctatgagagc gcggccaagg ccgtgatctt cctgcccatg gccatctcgt
     3541 tcgtggcggc gggggtcatc tggaagttca tgtaccagtt ccagccggcg gggaccgctc
     3601 aggtggggac cgtgaacgcc atcctgacgg cggttgcgcc ccggttccgg ccccaggcct
     3661 ggctggtcaa cgagtcggag aacaaccccg cgctgatcat cgtgggaatc tggatgtgga
     3721 cggggttcgc catggtgatc ctgtcggcgg ggttgaaggg gataccgacc tcggtgctgg
     3781 aggcggcgcg gatggacggg gcgggcgagc tgcagatctt cacgcgcatc acccttcccc
     3841 tcctggcgcc gacgcttacc gtggtcgcca cgacgctcat catcaacgtg ctcaaggtgt
     3901 tcgacatcgt gtacgtcatg accaacggga acctgaacac cgaggtcatc gccaaccgca
     3961 tgtacaagga gatgttcaac taccaggact tcggccgcgc cagcgcgttc gcggtcatcc
     4021 ttctccttgc cattgtgccg gtcatggtga tcaacatccg tcgcttcggc cgcagccggg
     4081 aggggatggc atgagcagcc gtgtgctcac ctccgcccgc ggacccgcca tgtcgccggt
     4141 ggcgcggctg ctcacccgtg gaccgctgca tgtcgtcctg gtggtggtag ggctgttgtg
     4201 gctcctgccg tccgtggggc tcctggtgtc ctccttccgt ccatccagcg tggtttcctc
     4261 caccggctgg tggatggcgt tcctgaaccc cttgcagttc acgctgagca actacgggta
     4321 tgtcctggga cgggcgaaca tggcgctcag cttcgccaac agcctggtca tcgccatccc
     4381 tgctatcgtg atgcccattc tgatcgtcgc cttcggggtg tatgtcttcg cgtggatgaa
     4441 gtttctggga tgcaacgtgc tctt
//
LOCUS       DASRZS010000055         4694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_419651, whole genome shotgun sequence.
ACCESSION   DASRZS010000055 DASRZS010000000
VERSION     DASRZS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419651"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..701)
                     /locus_tag="VFH83_01325"
     CDS             complement(9..701)
                     /locus_tag="VFH83_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6485027.1"
                     /translation="MSKIRVLIVDDQKLFVSSLTIVLRGHGKGNIEVVGAAYDGAEAQ
                     ELVAQTNPDIVLMDVRMPRMDGVKATRLIRERFPAVKIVILTTFDDDDYVFQALQNGA
                     VGYILKNIESEELVTAIKAVHNGQFLVSDAVGRKLINHVDLSEVQRAVSPEWESEVRA
                     LLAAFPVLTTREAEVLHLIARNWDNQEISERLYIAGQTVKNYTSRIYAKLGVSDRLHV
                     IQKVAKVMGGAA"
     gene            complement(694..1899)
                     /locus_tag="VFH83_01330"
     CDS             complement(694..1899)
                     /locus_tag="VFH83_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6485028.1"
                     /translation="MSTAFVKRPERISLFVFGFSMVFHGLAYLMLFTPRLGQGIAFPL
                     EYQVLIVGCAACSLVSLVIVSSAGFVAAHVVRAVFLFVVCRIAGPANLAVAGMLLLPY
                     LVETTLYLAAPWSLAINGIVIAANLGFDILRVAQDPTLEQILGLCAAYALYIATTVMG
                     NQLVSYRETIVAHTRRIANLTTANLAFQDHAEHVESESTKRERNRITRELHDITAYAL
                     TNIAMTMNAAKVLFTDNPRELPDLFETARRQAEDALQETRTTLYLLRSVEDRKLKGLH
                     AFVRMAREFQAATGVTVQINYGNVPFSLGPVVDAMIYRLIQQGLTNAFRHGKADTVRV
                     NLWRADAEIRVTISDNGKGGEELQEGIGMQGMRERLGMVGGSVEAHNRPSGFELSASI
                     PVEACDIDE"
     gene            complement(1900..3126)
                     /locus_tag="VFH83_01335"
     CDS             complement(1900..3126)
                     /locus_tag="VFH83_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6485029.1"
                     /translation="MNRVRRSWELARSQRAHLACGSSTNSKMPVFDDSEPALIVRGKG
                     CRVWDVDGNEYIDFRGGLGPVSLGYAVPEINAAIGRQLEDGICFGQPHPLEGEVAGLL
                     HEVVPCAEKVRFLKTGGEAVAACIKIARAATGRDLVVHCGYNGWLSNLARAKGTVPAG
                     IARSQPLKGVPQALSELHLSLPWAAEEEWRQLFAARGGEIAAVVVACDYAEMEKGTRF
                     LPLLRELTRASGSLLVMDEIVTGFRLAFGGAQEYFKVTPDLAAIGKGMANGMPLSAYA
                     GRADLMESACTLGISSTFGGEALSLAAAKATLAFYRQNGVIQHLWEMGRRLWPAAQRI
                     LERRAVAGRILGVPVCPSLTFDRKEQRDAFLKACYRHGVSLYDISYVTWSHRERDIEE
                     TLERMEAAVGELSPDR"
     gene            3251..4222
                     /locus_tag="VFH83_01340"
     CDS             3251..4222
                     /locus_tag="VFH83_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6485030.1"
                     /translation="MRLSVIGAGNHSALHHGAALAEYRRAHPGAIDLAGVCDLDAAKA
                     AEYARRFGFATTYTSASEMIAREKPDGLIVVTQPNVTAAIGPELLSRRIPLLLEKPPG
                     DTSDQTRALLRAAETGGTPHMVSLNRRYIPAVRKAREWLARRGETAAPRITIARMLRA
                     ARREAGFAVGTGIHVIDTVLCLMGAPRRAFTVKSAMESPGSFFFDTILDFGPGRSASI
                     VISPDVGTEEETFEIHGPGYCILIDSMRCSVRILEEKRETLSWAAAESDPFVFRCGSL
                     GETEAFVSAIREGNGFWPTLGDALVSMVACEAVDAGGEVSIPEQRES"
     gene            4219..>4694
                     /locus_tag="VFH83_01345"
     CDS             4219..>4694
                     /locus_tag="VFH83_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485031.1"
                     /translation="MRPFVTLKHAALLLLATVIPAGCFLLPKEEQKLAPPLMEPPQIT
                     YETITARRGTILDDFRVSGMLAYANQENLSFRSRTGRLLKRYVATGDRVKSGQLLAEL
                     DTDTLTYDISIQEIEVQKARLVAQRATLLNQDKIQQQLDALDVRKAEIELESLKGEL"
BASE COUNT          780 a         1592 c         1542 g          780 t
ORIGIN      
        1 ggtttgcttc atgccgcgcc tcccatcacc ttcgctacct tctggatcac gtgcaaccgg
       61 tccgacaccc ccagcttcgc gtagatccgg ctcgtgtagt tcttcacggt ctgtccggcg
      121 atgtacaggc gctccgagat ctcctggttg tcccagttgc gggcgatcag atgcagcacc
      181 tccgcctccc gcgtggtcag gacggggaag gcggccagca gggcccgcac ctcggactcc
      241 cactccgggg agaccgcgcg ctggacctcg ctcaggtcca cgtggttgat gagcttgcgg
      301 cccaccgcgt cggagaccag gaactgcccg ttgtgcaccg ccttgatcgc cgtaacgagc
      361 tcctccgact cgatgttctt gaggatgtac ccgaccgctc cgttctgcag cgcctgaaac
      421 acatagtcat cgtcgtcgaa cgtcgtaagg attacgatct tcaccgccgg gaaccgctcg
      481 cggatcaggc gggtggcctt caccccgtcc atgcggggca tgcggacgtc catgaggacg
      541 atgtccgggt tggtctgcgc aaccagctcc tgcgcctccg ccccgtcata ggcggcgccc
      601 accacctcga tgttcccctt tccgtgtccc cgcagcacga tggtaaggct gctcacgaac
      661 agcttctggt cgtccacgat caggacccgg atcttactca tcgatgtcac aggcctccac
      721 ggggatgctt gccgaaagct cgaaaccgct cggccggttg tgggcctcca cgcttccacc
      781 gaccatgccc agccgctccc gcatcccctg catgccgatc ccctcctgca gctcctctcc
      841 gcccttgccg ttgtctgaga tggtgacgcg gatctccgca tccgcgcgcc acaggttcac
      901 gcggaccgta tccgccttgc cgtggcggaa cgcgttggtg agcccctgct ggatcagccg
      961 atagatcatg gcgtccacca cgggacccaa ggagaagggg acgttcccgt agttgatctg
     1021 gacggtgacc ccggtggcgg cctggaactc ccgcgccatc cggacgaagg catggagccc
     1081 cttgagcttg cggtcctcca cggaccgcag gaggtagagc gtggtccgcg tctcctggag
     1141 ggcgtcctcc gcctggcgac gcgccgtctc gaagaggtcc ggcagctccc gggggttgtc
     1201 ggtgaagagg accttcgccg cgttcatggt catggcgatg ttggtgagcg cgtaggcggt
     1261 gatgtcgtgc agctcccggg tgatgcggtt gcgctcccgc ttggtggact cgctctccac
     1321 gtgctcggcg tggtcctgga acgcaaggtt ggcggtggtg aggttggcga tccgccgggt
     1381 gtgcgccacg atggtctccc gataactgac aagctggttg cccatgaccg tggtggcaat
     1441 gtacagcgca tacgccgcgc agaggcccag gatctgctcc agcgtcggat cctgggccac
     1501 tcgaaggatg tcgaagccga ggttggccgc gatgacgatc ccgttgatgg ccagggacca
     1561 gggggccgcg aggtaaaggg tcgtctccac caggtagggg aggagcagca taccagccac
     1621 ggcgagattg gcgggtcccg cgatccggca gaccacgaac aggaacacgg cgcgcacgac
     1681 gtgcgccgcc acgaatcccg ccgaggagac gatgaccagg gagaccaggg agcatgcggc
     1741 gcagcccacg atcaggacct ggtactccag ggggaacgcg atgccctggc ccaacctcgg
     1801 ggtgaagagc atcaggtagg caagcccgtg gaacaccatc gagaagccga acacgaagag
     1861 ggagatccgt tcgggcctct tgacgaacgc cgtgctcatc taccggtccg gtgagagctc
     1921 cccgacggcg gcttccatcc gctccagcgt ctcctcgatg tcacgctccc ggtgagacca
     1981 cgtcacgtag gagatgtcgt acagggagac gccgtggcgg tagcaggcct tgaggaacgc
     2041 gtcccgctgc tccttgcgat cgaaggtgag ggagggacac accggtacac ccaggatgcg
     2101 ccccgccacc gccctgcgct ccaggatcct ctgcgccgcc ggccaaagcc gccggcccat
     2161 ctcccagagg tgctggatca cgccgttctg ccggtagaac gccagggtgg ccttggcggc
     2221 cgccagggag agcgcctctc cgccgaacgt gctggagatg cccagcgtgc acgccgactc
     2281 catcaggtcc gccctgccgg cgtacgcgga gagaggcatg ccgttggcca tgcccttgcc
     2341 gatggcggcc aggtccggcg tgaccttgaa atactcctgg gcacctccga acgccagtcg
     2401 gaaccccgtc acgatctcat ccatcaccag gaggctgccg gatgccctgg tcagctcccg
     2461 aaggagcggg agaaaccggg tccccttctc catctcggcg tagtcacagg ccacgaccac
     2521 ggccgcgatc tctccgcctc gcgcggcgaa gagctgccgc cattcctcct cggcggccca
     2581 gggcagggag aggtgcagct cggacagtgc ctggggcacg cctttcagcg gctgggatcg
     2641 ggcgatgcct gccggcaccg ttcccttggc ccgagccagg ttggagagcc agccgttgta
     2701 gccgcagtgg accacgaggt cccggccggt cgcggctcga gcaatcttga tgcaggcggc
     2761 caccgcctcc ccgccggtct tgaggaacct caccttctcg gcgcacggca cgacctcgtg
     2821 caggaggccg gccacctctc cctccagggg atgcggctgc ccgaagcaga tcccgtcttc
     2881 gagctgacgg ccgatcgccg cgttgatctc cggtaccgcg tatcccaggg agaccgggcc
     2941 caggccgccc cggaagtcga tatactcgtt cccgtccacg tcccagacgc ggcaaccctt
     3001 gccgcgaacg atcagggccg gctcggagtc atcgaacacc ggcatcttgg agttggtgga
     3061 gcttccgcac gccagatggg ctctctggct gcgggcgagc tcccaggagc gccgcacgcg
     3121 gttcatgggg atcctcctcg cgatctcact gccgtagccg atcgcgggcg ggtcgtcaag
     3181 ggccgagcct caatcgcgtc gggcccggtc gctctggaca gaccgactgc gcctgaggat
     3241 gatgcggctt atgcgtctat cggtcattgg cgcgggaaac cattcggccc tccaccatgg
     3301 cgccgccctg gcggagtacc gcagagctca ccccggtgcg atcgacctgg cgggggtctg
     3361 cgatctggat gcggccaagg ccgcggaata cgcccgtcgg ttcggttttg cgacgacata
     3421 caccagcgcc tcggagatga tcgcgcgcga gaagccggac ggcctcatcg tggtcacgca
     3481 gccgaacgtg acggccgcca tcgggccgga gcttctttcg cggcgcatcc cgctcctcct
     3541 cgagaagccg ccgggggaca catcggacca gacccgcgcg ctcctccgag ccgccgagac
     3601 cggcggtacc ccgcacatgg tttcgctcaa ccgacgctac atcccggcgg tccgaaaggc
     3661 ccgggagtgg ctggcccgtc gcggggagac ggccgcgccc cgcatcacga tcgcgcgcat
     3721 gcttcgggcc gcccgcaggg aagccggttt cgcggtgggt accggcatcc acgtcatcga
     3781 cacggtcctg tgcctgatgg gcgctccccg acgcgcgttc accgtcaaga gcgcgatgga
     3841 aagcccgggg agttttttct tcgacaccat cctggacttc ggcccgggca ggagcgcttc
     3901 gattgtcatc tcgccggatg tgggcacgga ggaggagacc ttcgagatcc acggaccggg
     3961 gtactgcatc ctgatcgaca gcatgcgctg ctcggtccga atcctggagg agaagaggga
     4021 gacgctctcg tgggccgcgg cggaatcaga cccgttcgtc tttcgctgcg ggtccctggg
     4081 agaaacggag gcctttgtca gcgccatccg ggaaggcaac gggttctggc cgaccctggg
     4141 cgacgcgctc gtgtccatgg ttgcctgtga ggcggtggac gccggcggag aggtctcgat
     4201 cccggagcag cgcgagtcgt gaggccattc gtgaccctca agcacgcggc gctcctgctc
     4261 ctggcgacgg tgatccccgc cggctgcttc ctcctgccca aggaggagca aaagctggcg
     4321 cctcccctca tggagccgcc gcagatcacg tatgaaacga tcacggcccg gcgaggaacc
     4381 atcctggacg atttccgggt gagcggcatg ctggcgtacg ccaaccagga gaacctctcg
     4441 ttccgctcgc ggaccgggcg gctgctcaag cggtacgtcg ctacggggga ccgggtgaag
     4501 agcggtcagc ttctggcgga gctggatacc gacaccctca cgtacgacat ctccatccag
     4561 gagatcgagg tccagaaggc gcggctggtg gctcagcggg ccacccttct caaccaggac
     4621 aagatccagc agcagctcga cgccctggac gttcgaaagg cggagatcga gctggagagc
     4681 ctcaaggggg agct
//
LOCUS       DASRZS010000056         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_419911, whole genome shotgun sequence.
ACCESSION   DASRZS010000056 DASRZS010000000
VERSION     DASRZS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419911"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VFH83_01350"
     CDS             <1..723
                     /locus_tag="VFH83_01350"
                     /inference="COORDINATES: protein motif:HMM:NF023646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HET6485032.1"
                     /translation="YSMVKATRKSLEEIYRTLKPYHRVLTLACGGCTSVCLAGGQREA
                     LALNDELAELARRDRLAMQFSCFTTERQCNPEFVGDLKEMVAGCDCMLSTACGVGVQC
                     LAETYPATPAFPALDTLFLGADVDVGLYEERCRSCGECVLAVTGGICPVVRCSKGSFN
                     GPCGGTRCDGTCEVGGGLHCAWNDIYERLKAQNRVQAILEYRPPRNWNDKGPATLVQR
                     GYEARYADRLPKTEPGREGKAK"
     gene            720..1646
                     /locus_tag="VFH83_01355"
     CDS             720..1646
                     /locus_tag="VFH83_01355"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HET6485033.1"
                     /translation="MKSGSTLEKMLDAGHFAVTAELGPPSGSNGDVVRTKAELLRGVV
                     DGVNVTDNQTAIVRMSSLAACSILVQMGLEPVMQMVCRDRNRLAMQSDILGASALGVR
                     NLTCLSGDHQSFGNQQGARKVFDIDSVQLISIVKRIRDEGKLLDVEDELDGKVPMFIG
                     AASNPFAQPYAFRALRLGKKVAAGADYVQTQCIYDVKHFAEFMQAVRDLGLHHKVKIL
                     AGVTPLKSAGMTRYMAEKVPGIIIPPEIIQRMSAAPKGKGSEEGLKICAEIIEQLRAI
                     EGVAGIHIMAIEWEQKVRDIVQRAGLLPRPTV"
     gene            1780..2727
                     /locus_tag="VFH83_01360"
     CDS             1780..2727
                     /locus_tag="VFH83_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET6485034.1"
                     /translation="MQLDANDLQEAVALCDPDGIILEWNRAATEVTGFRAEEVIGYHL
                     DAVLAPSSQETIRDLFNAERTGSVLPGLAVRLQTNYGMEVPAEVTTAPQFSEGSLRGW
                     VLIFRDTTLRVMLREELDRMETLYRGLVERSPDIIYVLDVQGRLLFINDTVETLLGYP
                     KKDLLGRELIDIVHPDDRDRAYWPLRERRRADRATRSLRLRMITGAGAPRRYDLDFVY
                     ISLDSVGLSDPRPPLQNGLAGEGLGTQGVARDITDLVMLQEFAGQVDLILPVCAICRK
                     IRVQSGGQEQWLPLSEYLGIKTGILFSHTYCPDHMPRAE"
     gene            complement(2743..>3313)
                     /locus_tag="VFH83_01365"
     CDS             complement(2743..>3313)
                     /locus_tag="VFH83_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485035.1"
                     /translation="PSLIYRNHVHRTSHQVTARLFKEYRPQVICGGHGLYTNVAPEGY
                     DLFLANAERLTELFDTLLPKDSGILGLEPSWIQIFPYQLSGQPGDLVQGQIRIRNPVP
                     HRAEIRWKWALPDGWEVKPAQGIAHVEPEESTRSPFTISIAQTYRFSHPKQAIALDVT
                     LDGKALGQVAEAVVENLPYGGAGAPPPAR"
BASE COUNT          553 a         1099 c         1109 g          552 t
ORIGIN      
        1 tactccatgg tcaaagcgac ccgcaaatcc ctcgaagaga tataccggac cctgaagccg
       61 taccaccgcg tgctcaccct ggcctgcggc ggctgcacct ccgtgtgcct ggccggcggc
      121 cagcgggaag cgttggcgct caacgacgag ctggccgagc ttgcccgtcg ggaccggctc
      181 gccatgcagt tcagctgctt caccacggag cggcagtgca accccgagtt cgtcggggac
      241 ctcaaggaga tggtggccgg ctgcgactgc atgctctcca cggcttgtgg ggtgggcgtg
      301 cagtgcctgg cggagactta cccggccacg ccggccttcc cggcgctgga cacgctgttt
      361 ctcggcgcgg acgtggatgt gggcctctac gaggagcgct gccgatcctg cggcgaatgc
      421 gtcctggccg tgaccggcgg catctgcccc gtggtccgct gctccaaggg gagcttcaac
      481 gggccctgcg gcggcacgcg gtgtgacggc acctgcgagg tggggggcgg tctgcactgc
      541 gcctggaacg acatctacga gcgcctgaag gcccagaacc gcgtgcaggc catcctcgag
      601 taccggcctc cccgcaactg gaacgacaag gggccggcca ccctggtgca gagaggctat
      661 gaggctcggt acgcggaccg actgccgaag acggagccgg gtcgggaggg gaaggcgaaa
      721 tgaaatccgg cagcaccctg gagaagatgc tcgacgccgg ccacttcgcg gtcaccgcgg
      781 agctgggtcc ccccagcggc tcaaacgggg acgtggtgcg caccaaggcc gagctcctgc
      841 gcggcgtggt ggacggcgtg aacgtcacgg ataaccagac cgccatcgtc cgcatgtcca
      901 gtctggccgc ctgctcgatc ctggtgcaga tggggctgga gccggtgatg cagatggtct
      961 gccgggaccg caaccgcctg gccatgcaga gcgacatcct gggcgcttcc gccctcggcg
     1021 tccgcaacct gacctgcctc tccggggacc accagagctt cggcaaccag cagggcgccc
     1081 gcaaggtctt cgacatcgac tccgtgcagc tcatctcgat cgtcaaacgg atccgggacg
     1141 aaggcaagct cctggacgtg gaggacgagc tggacggcaa ggtccccatg ttcatcgggg
     1201 ccgcctcgaa ccccttcgcc cagccctacg cgtttcgggc cctgcggctg gggaagaaag
     1261 tcgcggcagg cgcggattac gtccagaccc agtgcatcta cgacgtgaag cacttcgcgg
     1321 agttcatgca ggcggtccgc gacctcgggc ttcaccacaa ggtgaagatc ctggccggcg
     1381 tgaccccgtt gaagtccgcg ggcatgaccc gctacatggc cgagaaggtg cccgggatca
     1441 tcatcccgcc ggagatcatc caacggatgt ccgccgctcc caagggaaag ggatccgagg
     1501 aagggctcaa gatctgcgcg gagatcatcg agcagcttcg cgccatcgaa ggggtggccg
     1561 gcatccacat catggcgatc gagtgggagc agaaggtccg cgatatcgtc cagcgggccg
     1621 ggctcctgcc ccggcccacc gtctagcgag cctcccgggg ctgggaagcc gccccggcgc
     1681 tctccatccg taccgctcga aaggttctcc cctgttcctg agcctctcgg cgtgctacag
     1741 ttctggcggt ctgctctcca gggaaaacgg gaggttttga tgcagctgga cgcgaacgat
     1801 ctacaggagg ccgttgcgct ctgcgacccg gacggtatca tcctcgaatg gaaccgcgcc
     1861 gccacggagg tgacggggtt ccgcgccgag gaagtgatcg ggtaccacct ggatgccgtc
     1921 ctggccccca gctcccagga gaccatccgc gacctgttca acgccgagcg cacgggatcc
     1981 gtgctcccgg gcctcgccgt ccgactgcag accaactatg gcatggaggt ccccgcggag
     2041 gtcactaccg cgccccagtt ctccgaggga agcctgcgcg gatgggtcct gatcttccgc
     2101 gacaccaccc tgagagtgat gcttcgcgag gagctggatc ggatggagac tctctaccgc
     2161 gggctggtgg agcgctctcc ggacatcatt tatgtcctgg acgtgcaggg aaggcttctc
     2221 ttcatcaacg acacggtgga aaccctgctg ggatacccga agaaggatct gttgggccgg
     2281 gagctgatcg atatcgtcca tcccgacgac cgggaccgcg cctactggcc tctccgcgag
     2341 cgccggcgcg cggatcgcgc cacgcggagc ctgcggctgc ggatgatcac gggcgccggc
     2401 gccccgcggc gttacgacct cgacttcgtc tacatctccc tggactccgt ggggctgtcg
     2461 gatcccaggc cccctttgca gaacggtctg gccggagagg gcctgggaac ccagggtgtg
     2521 gcccgagata tcacggacct cgttatgctg caggagttcg ccggtcaggt ggacctgatc
     2581 cttcccgtct gcgccatttg ccgcaagatc cgagtccagt ccggaggcca ggagcaatgg
     2641 ctccccctct cggagtacct ggggatcaag accgggatcc tcttctctca cacgtactgc
     2701 cccgaccaca tgccgcgggc ggagtaggcc cgcgggcgca agctaccgcg caggtggcgg
     2761 cgcgcccgcc cctccgtacg gcagattctc tacgacggcc tcagccacct ggcccagcgc
     2821 cttgccgtcc agcgtgacat ccagcgcgat ggcctgcttg ggatgcgaga accgataggt
     2881 ctgcgctatc gagatggtga acggggagcg cgtgctctcc tcgggctcga cgtgcgcgat
     2941 cccctgcgcc ggcttgactt cccagccgtc cggtagcgcc cacttccagc gaatctccgc
     3001 tcggtgcgga accggattcc tgatccggat ctggccctgg accaggtccc ccggttggcc
     3061 tgagagctgg tacgggaaga tctggatcca gctcggctcg agccccagga ttccgctgtc
     3121 cttcggcagg agggtgtcga agagctcggt caggcgctcc gcgtttgcca ggaacaggtc
     3181 gtatccttcc ggcgccacgt tggtgtaaag gccatggccg ccgcagatga cctggggtcg
     3241 gtactccttg aagagccgcg cggtgacctg gtgactggtg cggtggacgt ggtttcggta
     3301 gatgaggctg gga
//
LOCUS       DASRZS010000057         7287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4268797, whole genome shotgun sequence.
ACCESSION   DASRZS010000057 DASRZS010000000
VERSION     DASRZS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4268797"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VFH83_01370"
     CDS             complement(<1..540)
                     /locus_tag="VFH83_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485036.1"
                     /translation="MKNIPVLHASGKSLAECYERALLALYEGGVRIQTQYDKPGDPQS
                     IDATMNLTVEEPWSDPMIHKAFPGGIEDLREYVMELSGAKDHWVKNLDDPADTRWEYT
                     YHQRLADWGTWRQRAPQTAGRPGTGVDQISAVVDKLARQPHTRQAQMITWMPNLDLDC
                     YDPPCLQSLWYRLVDDPDTG"
     gene            635..2173
                     /locus_tag="VFH83_01375"
     CDS             635..2173
                     /locus_tag="VFH83_01375"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET6485037.1"
                     /translation="MAKSRIMVVEDEGVVALQIKEALTNLGYEVAAVAMTGDEAVSKF
                     QETEPDLVLMDVRLRGGVSGIEAAKRIRTRLDVPVVYLTAYADDETLHQAQLTEPYAY
                     VLKPFDEKSLHAVIQMALLKSRRARSALETNWWTTAIASSMMEAVVICDPKGYIKFVN
                     RAAEALLGKTSFEVLEHRLAETVTLQDSVTRSPLTFPVTEPLLEGRSTLKGNCRLLVG
                     ERDVPVEFSASPLKSPEGTQFGVMYVFRETGEREKIQNLVLRELEELAKVQKKVLPSG
                     DTEIQGVSFRWLFYSTPIGGGDGLGFYPLDPGHIGFHALDVIGHGILSALFSLLVHSY
                     LSPRSDRGGILVESIREKPGHRIIPPEEVVKALDRRFYLRDEANPYFTFVYGVMDTAT
                     GVLRLVRAGHPYPILHTAMGQQRALRPDGYAIGLFPGTAIKAEELTLGHGDRLYLYSD
                     GLLDLESPKGERFGPQRLAELIAMQRKKTLEDAIQSIQEGVLSWRGDSALADDVSLLA
                     LERS"
     gene            complement(2225..4327)
                     /gene="glgX"
                     /locus_tag="VFH83_01380"
     CDS             complement(2225..4327)
                     /gene="glgX"
                     /locus_tag="VFH83_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET6485038.1"
                     /translation="MAWVKLCYPPVGPMRTATHTTPGKPIPYGATPGPRGTQFSVFSR
                     NATAVSLLIFDGPQDAAPALVVPLEPGVNKTGDMWHVEVAGVGPGAFYLYRVDGPYDP
                     HRGDRFDPHKPLLDPYVKAIAPSESGGTPKGIVVSDDFDWRGDRPLGRPLRTSVIYET
                     HVRSLTCGPGAGVEHPGTFRGVAEKLPYLRDLGITAVELLPVQEFDERENPRHNPVTG
                     ERLQNYWGYSTIGFFAPKGGYSSAGALGQQVTEFKEMVRDLHAAGIEVILDVVFNHTA
                     EGNETGPTLSFRGLDNSIYYMLDGRDRSRYRNFSGCGNTLNCNHPIVRSLIIDCLRYW
                     VVEMHVDGFRFDLGSVLGRDIDGNLLPNPPILQRIAEEPVLRHTKIIAEAWDAAGAYQ
                     VGWFPGGRWAEWNDRFRDDVRRYWRGDSGMVPGLATRLAGSSDLYLRDGRKPFHSVNF
                     ISSHDGFTLNDLVSYSRKHNEENGEGNQDGSTPDQAANYGVEGPTDDPRIESIRNRQV
                     KNLLATVLLSLGTPMLLGGDEIRRTQRGNNNPWCQNNLISWYDWSFLERHADIHRFCR
                     QLIRFRLAHPAFLRPEFYSGRDASHNRIPDITWLTEEAEPADWAPDRSTLAILIDGNK
                     ADIEADRDDNDILMLFNCRDQPVVFVLAPVPPGKEAWYRVMDTSIARDVASPGEEERM
                     VGETYRMMDRSLAVFLSR"
     gene            4348..5730
                     /locus_tag="VFH83_01385"
     CDS             4348..5730
                     /locus_tag="VFH83_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485039.1"
                     /translation="MDRTTLSREIQSALEEIPLLDAHTHLDATHLAARGLHDLLLYHM
                     VISDLSSAGCPDRSRLPEAPTEEEARSRIERALPYLPAIANTSCFWGLRLILKDLYGW
                     DKPVTAGNWRKLDASIRERSGDPRWPREILKKAGIVRSSTEYWRRHDGSVDELLQYSL
                     EWAFFGRASWGEFDTGLYELEKAWSEGVPSPPSPIATGGRPVPSRTIRTVRDVHEALD
                     AYVDAIPSNVLSTAQHISTDIDFKPVDEKTMAAAIGRRSRAAPAERDIYASYTTEEFW
                     KRLEARRGGVVYQFSLGAEPLPFETSSRIYQRTLVFLAEAIARHPGIRFQCFLGSSHA
                     NQTLCTIARELPNLSLAGYWWHNFFPTVIERVMRERLDMLSTARQIGFFSDAYTLEWS
                     YAKAVMVRRILSGVLADKVLEGQYDRKTAIDTARRILFDSPRELLGMEPAVTSVRAGA
                     QLKTRRPRGG"
     gene            5736..6677
                     /locus_tag="VFH83_01390"
     CDS             5736..6677
                     /locus_tag="VFH83_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6485040.1"
                     /translation="MAAIDVLGLGAVAVDDILFLPSFPKPDSKVPILGRERHPGGLSG
                     TALVAARRMGRAAAYAGTLGDDELSRFILEGFRVEGVSVEHVVRRDGTRPFHSTILVE
                     QANHTRTILLNTQDVAGADPSAPPEAIIRGARVLLVDHIGMEGMVRAARIARSAGIPV
                     VADLERDAGPLAQELIGLVDHLILPFEFARKLSGGASVQDIVQRLWSDRRAAVVITRS
                     EDGSWYQSAEEPGLFFHQPAFRVDPVDTNGCGDVFHGVYAAGLAERLPIAERVRLASA
                     VAALKATQAGGQQGIPTREAAERFLSERSAEARRAPA"
     gene            complement(6725..>7287)
                     /locus_tag="VFH83_01395"
     CDS             complement(6725..>7287)
                     /locus_tag="VFH83_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET6485041.1"
                     /translation="RSEAWELFCKHNATESLRGHALSVEAVMRYMARKYGEDVEKWGI
                     IGLVHDIDYEQFPQEHCKRSPTILSEAGWPEEYIHAVVSHGWGICSDVEPTHRMEKVL
                     FAVDELTGLVKASALMRPSRSVLDMEASSVKKKWKDARFAAGVNRSIIEKGAGMMGVE
                     IGDLITDTILGMRDAAEAIGLKGSVA"
BASE COUNT         1187 a         2426 c         2426 g         1248 t
ORIGIN      
        1 gcccgtgtcg ggatcgtcca cgagccggta ccagagcgat tggaggcagg gcgggtcgta
       61 gcagtcgagg tcgaggttgg gcatccaggt gatcatctgc gcctggcggg tgtgcggctg
      121 tcgggcaagc ttgtccacca ccgcggagat ctggtccacg ccggtccccg gacggccggc
      181 ggtctgcggt gcccgctggc gccacgtgcc ccagtccgcc agccgctggt ggtaagtata
      241 ctcccaacgg gtgtcggcgg ggtcgtcgag gttcttcacc cagtggtcct tggcgcccga
      301 aagctccatc acgtactccc gcaggtcctc gatgccgccg gggaacgcct tgtggatcat
      361 cggatccgac catggctcct ccaccgtgag gttcatcgtt gcatcgatgg actgggggtc
      421 ccccggcttg tcgtactggg tctggatgcg gacccctccc tcatagagcg ccagcagggc
      481 gcgctcgtag cactccgcga gggatttgcc gctcgcatgg aggaccggga tattcttcat
      541 ggacgcctac tctgacacgg cgaagaaccc ctgtcgagaa gcggttgtgc agagcgtcat
      601 ggcggggtag agtgatgcca ggtgcgagga aagtatggcc aaatccagaa tcatggtggt
      661 cgaagacgaa ggcgtcgtgg cgctccagat caaggaggcg ctcacgaacc tcggctacga
      721 agtggccgcc gtggcgatga ccggggacga ggccgtctcc aagttccagg agacggagcc
      781 ggacctggtg ttgatggacg tgcgccttcg gggcggcgtg agcggcatcg aggccgccaa
      841 gcgcatccga acgcgcctgg acgtgcccgt ggtctatctg accgcctacg cggacgatga
      901 gacgctgcac caggcgcagc tcaccgagcc ctacgcctac gtattgaagc cgttcgacga
      961 gaagagcctg cacgcggtca tccagatggc gctcctcaag tcccgacgcg cgcgaagcgc
     1021 cctggagacc aactggtgga ccacggccat cgcctccagc atgatggagg ccgtggtcat
     1081 ctgcgatccc aagggctaca tcaagttcgt gaaccgcgcg gccgaggccc tgctgggcaa
     1141 gacttcgttc gaggtgctgg agcatcggct ggcggagacg gtcacgctgc aggactcggt
     1201 cacgcgcagt cctctcacct ttcccgtgac cgagcccctc ctggagggga ggagcaccct
     1261 caaggggaac tgccgtctgc tggtgggaga gcgcgatgtg cccgtggagt tcagcgcctc
     1321 tcccctgaag agcccggagg gcacgcagtt cggcgtcatg tacgtcttcc gagagaccgg
     1381 cgagcgggag aagatccaaa acctggtcct gcgggagctg gaggagctgg cgaaggtcca
     1441 gaagaaggtc ctgccttccg gcgacacgga gatccagggc gtgagcttcc gctggctctt
     1501 ctactcgacg ccgatcggag ggggagacgg gctgggcttc tatccgctgg acccgggtca
     1561 catcgggttc cacgcgctgg acgtcattgg acacggcatc ctctccgccc tgttctcgct
     1621 tctcgtgcac tcctacctga gcccgcggtc ggaccgcggc ggcatcctgg tggagagcat
     1681 ccgtgagaag ccggggcacc ggatcattcc gcctgaagag gtcgtgaaag ccctggaccg
     1741 gcgattctac ctgcgggacg aagccaaccc gtacttcacc ttcgtctacg gcgtcatgga
     1801 tacggccacc ggggtcctgc ggctggtgcg cgcgggccac ccgtatccca tcctccatac
     1861 ggcgatgggg cagcagcggg ccctgcggcc ggacggatac gccatcggcc ttttccccgg
     1921 gaccgccatc aaggcggagg agctgaccct cggtcacgga gaccggctgt acttgtactc
     1981 cgatgggctc ctggatctcg agagccccaa aggggagcga ttcggaccgc agcggcttgc
     2041 cgagttgatc gccatgcagc gcaagaagac cctggaagac gccatccaat ccatccagga
     2101 aggtgtcctg agctggcgcg gtgacagcgc cctggcggac gacgtctccc tgcttgccct
     2161 cgagcggagc tgagtctcgt cctggttcct cggaccgcgg cgacgccggc ctctgggcgc
     2221 ggcctcagcg ggacaggaac acggccagcg atcgatccat cattcgatag gtctccccaa
     2281 ccatccgctc ttcctctcct ggcgaggcga cgtctcgggc gatgctcgtg tccatcaccc
     2341 gataccaggc ctccttgccg ggaggcaccg gagccaggac gaacaccacg ggctggtccc
     2401 tgcaattgaa gagcatcagg atgtcgttgt cgtcgcggtc cgcctcgatg tcggccttgt
     2461 tcccatcgat gagaatcgcc agggtcgagc gatccggcgc ccaatccgcc ggctccgcct
     2521 cctcggtcag ccaggtgatg tccggaatgc ggttgtggga ggcgtctcga ccgctgtaga
     2581 actcgggcct caggaacgcg gggtgagcca ggcggaagcg gatgagctgc cggcagaacc
     2641 ggtgaatgtc cgcgtgccgc tcgagaaagc tccagtcgta ccaggagatg aggttgttct
     2701 ggcaccacgg gttgttgttc ccacgctggg tgcggcgaat ctcgtctccc cccagaagca
     2761 tcggggttcc cagggaaaga aggaccgtgg ccaggaggtt cttcacctgg cggtttcgga
     2821 tggactcgat ccgcggatca tccgtgggac cttccacgcc gtagttcgcg gcctgatccg
     2881 gcgtggatcc gtcctggttc ccttccccgt tctcctcgtt gtgcttgcgg ctgtagctca
     2941 cgaggtcgtt cagcgtgaac ccgtcgtggg aggagatgaa gttgacgctg tggaacggct
     3001 ttcggccgtc ccgcagatag aggtcggagc ttccggccag tcgggtggcc agcccgggca
     3061 ccatgccgga atctccgcgc cagtaacggc gaacgtcgtc gcgaaaccgg tcattccatt
     3121 ccgcccaacg gccaccggga aaccagccga cctggtaggc gccggcggcg tcccacgcct
     3181 cggcgatgat cttggtgtgc cggagcacgg gctcttccgc gatgcgttga aggatgggcg
     3241 ggttcggcag caggttgccg tcgatgtcgc ggcccagaac ggagcccagg tcgaatcgaa
     3301 acccgtccac gtgcatctcc accacccagt agcgcaggca gtcgatgatg aggctccgga
     3361 cgatgggatg gttgcagttc agcgtgttgc cgcacccgct gaagttccga taccggctgc
     3421 ggtcccgccc gtccagcatg tagtagatgg agttgtccag gccccggaag gagagcgtgg
     3481 gcccggtctc gtttccctcc gccgtgtggt tgaagaccac gtcgaggatc acctcgatac
     3541 cggccgcgtg caggtcccgc accatctcct tgaactccgt cacctgctga cccaaggcgc
     3601 cggcgctgga atacccgccc ttgggcgcga agaagccgat ggtgctgtat ccccagtagt
     3661 tctgaagccg ctcgccggtg acggggttgt gtcgggggtt ctccctctcg tcgaactcct
     3721 gcaccggcag gagctcgacg gctgtaatgc ccaggtcgcg tagatacgga agcttctccg
     3781 cgactccccg gaatgttccc gggtgctcca ccccggcgcc ggggccgcag gtgagcgaac
     3841 gcacgtgggt ctcgtagatc acgctggtcc gaagcggcct tcccagcggc cggtcgccgc
     3901 gccagtcgaa atcgtccgag acgacgatac ccttcggggt gcctccgctc tccgagggcg
     3961 cgatggcctt gacgtacgga tccagcagcg gcttgtgggg gtcgaaccgg tcgccgcggt
     4021 gtgggtcgta ggggccgtcc acgcggtaca ggtagaaggc cccggggccg accccggcca
     4081 cttccacgtg ccacatgtcg ccggtcttgt tcaccccggg ctccaggggc actaccagcg
     4141 ccggtgccgc gtcctggggc ccgtcgaaga tcaacagaga cacggcggtc gcattacggc
     4201 tgaacaccga gaactgcgtg cctcgagggc ctggcgtcgc cccgtaggga atcggcttcc
     4261 cgggcgttgt gtgggttgca gtgcgcatcg gaccgacggg aggatagcat agcttgaccc
     4321 acgccatcaa acaccggaca ataccctatg gaccgcacga cactgagtcg cgaaatccag
     4381 agcgcgctgg aagagatccc cctcctggac gcgcacaccc acctggacgc aactcacctt
     4441 gcggcgcgtg ggctgcacga ccttctcctg taccacatgg tgatcagcga cctgtccagc
     4501 gcgggctgcc ccgaccggtc ccgtctgccc gaggcgccga cggaagagga ggcgcgttcg
     4561 cggatcgagc gggcgctccc gtaccttccg gccatcgcca acacaagctg cttctggggg
     4621 ctgcggctca tcctcaagga tctctacggc tgggacaagc cggtcaccgc gggcaactgg
     4681 cggaagctcg acgcctcgat ccgcgagcgc agcggggacc cgcggtggcc ccgcgagatc
     4741 ctgaagaagg cggggatcgt acggagctcc acggagtact ggaggcgaca cgatggatcc
     4801 gtggacgagc tgctgcagta ctccctggag tgggccttct tcggccgggc gtcgtgggga
     4861 gagttcgata ccggtctcta cgagctggaa aaggcatggt ccgaaggcgt gccgtctcca
     4921 ccgtctccca ttgcgaccgg cggaaggccc gtgccgtccc gaacgatccg cacggtccgg
     4981 gacgtgcacg aagccctgga cgcatacgtg gatgccatcc cctccaacgt cctgtccacg
     5041 gcgcagcaca tctccacgga tatcgacttc aagccggtgg atgagaagac gatggctgcc
     5101 gccatcggcc gaagatcccg ggccgctccg gcggagcgtg acatctatgc ctcctacacc
     5161 acggaggagt tctggaaacg gctggaggcg cggcgcggcg gcgtggtcta ccagttctcc
     5221 ctgggcgcgg agccgcttcc cttcgagacc tccagccgga tctaccaacg gaccctggta
     5281 ttcctggccg aggccattgc ccggcatccc ggtatccgct tccagtgttt cctgggttcg
     5341 agccatgcca accagaccct ctgcaccatc gcccgcgagc tccccaacct gtccctggcc
     5401 ggctactggt ggcacaactt cttccctacg gtgatcgagc gggtcatgcg ggagaggctg
     5461 gatatgctct ccacggcccg gcagatcgga ttcttctccg acgcctacac cctggagtgg
     5521 agctacgcca aggccgtcat ggtccgccgc atcctgagcg gcgtgctggc cgacaaggtc
     5581 ctggagggcc agtacgaccg aaagacggcc atcgacaccg cccggcgcat cctcttcgac
     5641 agcccacgtg agctccttgg catggagccc gccgtcacgt cggtccgggc gggcgctcag
     5701 ctcaagaccc gaaggccgcg gggagggtag gccgggtggc agccatcgat gtgctcggtc
     5761 tcggggccgt cgcggtcgac gacatcctgt tcctccccag cttccccaag ccggacagca
     5821 aggtccccat cctgggcaga gagcgccacc caggcgggct ctccggaacg gccctggtgg
     5881 ccgcgcggcg catgggccgc gccgccgcct atgcggggac cctgggggac gacgagctct
     5941 cccgcttcat cctggaggga ttccgcgtgg aaggggtttc cgtcgaacac gtcgttcgac
     6001 gggatgggac gaggccgttc cactccacca tcctcgtcga gcaggccaac cacacgcgga
     6061 ccatcctgct caacacccag gacgtggcgg gcgcggatcc gtccgctcct cccgaggcga
     6121 tcatccgggg cgcccgcgtg ctcctggtgg atcacatcgg tatggaaggg atggtgcggg
     6181 cggcgcggat cgcccggagc gcgggcatcc cggtggtcgc cgacctggag cgggacgccg
     6241 gacccctggc gcaggagctg atcggcctgg tagaccacct gatcctgccg ttcgagttcg
     6301 cccggaagct gagcggcggc gcctccgtcc aggatatcgt ccagcgcctg tggtccgacc
     6361 ggcgcgcggc cgtggtgatc actcgtagcg aggacgggtc ctggtaccag agcgcggagg
     6421 agccggggct cttcttccac cagcccgcct ttcgggtgga cccggtggac accaacggct
     6481 gcggcgacgt gttccacggc gtatacgccg cgggtctcgc cgagcgcctg cccattgccg
     6541 agcgagtgag gttggcctcc gcggtcgccg ccctcaaggc gacgcaggcc ggagggcagc
     6601 agggcattcc gacgcgggaa gcggctgaac ggttcctctc cgagcgctcg gcggaagccc
     6661 gccgcgctcc ggcctagggc aaccgggagc tgtcaggcta ccgagcctcc cggcggccgc
     6721 gccctcaggc caccgagccc ttcaggccaa tggcttccgc ggcgtcgcgc atacccagga
     6781 tggtgtccgt gatgagatcg ccgatctcca cgcccatcat cccggcgccc ttctcgatga
     6841 tgcttcggtt gacgccggcg gcgaaccgcg cgtccttcca cttcttcttc accgaggagg
     6901 cctccatgtc cagcacgctg cgcgacggcc tcatgagcgc ggaggccttc accagtccgg
     6961 taagctcgtc cacggcgaag agcaccttct ccatccggtg ggttggctcc acatcggagc
     7021 agatccccca tccgtggctg accacggcgt ggatgtactc ctccggccag cccgcctcgg
     7081 agaggatggt cgggcttctc ttgcagtgct cctgggggaa ctgctcgtag tcgatgtcgt
     7141 ggaccagtcc gatgatcccc cacttctcga cgtcctcgcc gtacttgcgg gccatgtacc
     7201 gcatcacggc ctccacggac agggcgtggc ctcggaggct ttcggtggcg ttgtgcttgc
     7261 agaaaagctc ccaggcttcg ctgcggg
//
LOCUS       DASRZS010000058         7563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774 k141_33633,
            whole genome shotgun sequence.
ACCESSION   DASRZS010000058 DASRZS010000000
VERSION     DASRZS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33633"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1126)
                     /gene="gap"
                     /locus_tag="VFH83_01400"
     CDS             complement(65..1126)
                     /gene="gap"
                     /locus_tag="VFH83_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET6485042.1"
                     /translation="MAVKVGINGFGRIGRQVFLAMVDKGLLGKEIDVVAVVDITTEAK
                     YFAYQLKYDSVHGRFQGTLATEKSPGAAEDDILIVNGNKTKCILATKEPAQLPWKDLG
                     VEYVIESTGLFTESDKAKGHLAAGARKVIISAPGKGEVKTLLMGVNDNEYDPKKHDIV
                     SNASCTTNCLAPLVHVILKEGIGIETGLMTTIHSYTATQKTVDGPSKKDWRGGRAAAI
                     NIIPSTTGAAKAVGEVLPAVKGKLTGMSFRIPTPDVSVVDLTFRSARDTSIEEIDSLL
                     KKASQTYLKGILGVADEEVVSTDFIHDNRSSIYDSLATLQNNLKGEKRFFKVVSWYDN
                     EWGYSSRTVDLLMKMAKTK"
     gene            complement(1272..1718)
                     /locus_tag="VFH83_01405"
     CDS             complement(1272..1718)
                     /locus_tag="VFH83_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET6485043.1"
                     /translation="MINLKDYIAKERIAFLEARDKQDALAKLIALSDDPRHVIDFEQF
                     QNAVLDREYIVTTGIGQNVAIPHIKSRWVRDFFISIGVFRDGVEWQSLDEKPVHLAFL
                     IAGPEEHENYLRILAKLTLIIRDKYKRQSIIKATDADAVYRLLEDS"
     gene            complement(1836..2951)
                     /locus_tag="VFH83_01410"
     CDS             complement(1836..2951)
                     /locus_tag="VFH83_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HET6485044.1"
                     /translation="MIRLGDRVVGSGAPALIIAELGTSHQGDLSRARSMIDAAVDAGA
                     DCVKFQLVYATELLHPRSGLVDLPTGRIPLFERFRELERDLSFYAALKTHTESRGALF
                     LCSPFGIQSARELHRLGAEAIKVASPELNHLPLLQEVSAFGVPLFLSSGVSTLADIEQ
                     ALSITGRNNVVLLHCITAYPAPEEQYNVSVLRSLRAVFGVEVGLSDHSLDPELVPTAA
                     AACGAVAVEKHFTLSREGSGLDDPIALEPRDFSRMVAAVRAAERLGPEAAIAGLGARY
                     GRQRVDAVLGSGIKTLAAAERENYRRTNRSIHALREIPQGAVIRSQDVALLRTEKVLR
                     PGLPPELLPRVIGAHAMRLIPDGEGVEWEDILPSQGS"
     gene            complement(2948..4141)
                     /locus_tag="VFH83_01415"
     CDS             complement(2948..4141)
                     /locus_tag="VFH83_01415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6485045.1"
                     /translation="MSTTQKMTPAHRWLFILLWCGFAVYGAVLTTFGATVPRIIQDLG
                     WSYATTGLVLAATSIGFFFSTFAVGLLLEGTNPKGVYVVAVIGTAVALSLFGRSASPW
                     VNIVVCIVSGAGQGVIEVATQYEVVRIEKPGESRLMNLLHAGFSAGAIVGPIGVGALM
                     QSGLSWKIAFPACGVLFVILALWALATRFPAPERGKHHGAAGGVALLRQPVLLLLCVV
                     IILYVGSELGATNWVAEYLVRVIGASGPVSALGVSVLWGGMMIGRTILSRARQVRRQE
                     LLLAGLAVVCVAALAGFLASRSLLPAVVMIFALGLGYSGLYPIVMAIAGRTFRSSAAV
                     GMVSTSAGIGSFSFPFLLAAVSQAAGLRWGFVMLTVLPLGILVASLSMIGRIPAQDQS
                     AGAAG"
     gene            complement(4138..4677)
                     /gene="dcd"
                     /locus_tag="VFH83_01420"
     CDS             complement(4138..4677)
                     /gene="dcd"
                     /locus_tag="VFH83_01420"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010275257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HET6485046.1"
                     /translation="MILSDGTLRRMMAERSIIVEPIEPYQLQPASVDLRLGNHFLKID
                     ENTLESLSLDREPPYVRIDKEEIVIPPHSFLLATTVEYVKIPTNVTAFVEGRSSIGRM
                     GLFIQNAGWVDPGFEGTITLELYNANRLPIRLACGRRICQIVFALMDQPAGRPYAGKY
                     QGQRGTVGSRIDRDTEGSA"
     gene            complement(4674..6140)
                     /locus_tag="VFH83_01425"
     CDS             complement(4674..6140)
                     /locus_tag="VFH83_01425"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET6485047.1"
                     /translation="MTARERDPSLTLLTDFYELSMMQGYFLHQRNPHVVFDLFFRRAP
                     FGGGFSVFAGLETALRFVQDIRFTEEDIAYLRGLGTFRPEFLHYLEGMRFRGDIWAME
                     EGTLAFPGEPLLRVHATLIEAQLLESALLAIVNFQTLIATKAARVRLAANGRSVIEFG
                     LRRAQGIDGALSASRAAFIGGVSATSNTLAGKRFSIPVRGTMAHSWVMVFENEREAFE
                     RYADAYPDNALLLIDTYDTLGSGLQNAVAVGRRLKAAGHRGFGVRLDSGDLEYLSKRV
                     RARLDAEGLADALIVASNDLDEAIIHQLITRGAPIDVWGVGTNLVTGAGDPALTGVYK
                     LAARERERDGIFEPTIKVSNNPEKVTNPGVKQVWRFVTPEGSPLADLICLEDETLETG
                     RPYTFHHPHGDYRSFTLREYGRMTALLKRWMTAGEIVGELPSLPDLQARTLRHLQALD
                     ETYKRLINPHDYKVSLSSRLSELKAGLIRQHLTQGEGS"
     gene            complement(6151..6223)
                     /locus_tag="VFH83_01430"
     tRNA            complement(6151..6223)
                     /locus_tag="VFH83_01430"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6188..6190),aa:Ala,seq:cgc)
     gene            complement(6360..6773)
                     /locus_tag="VFH83_01435"
     CDS             complement(6360..6773)
                     /locus_tag="VFH83_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485048.1"
                     /translation="MARKRVSFDVVVVIQIVLAVFLISLGVVGINDWNSNASQFSRGL
                     TRFFGGTNNPFNLIVAICELAAGVIVFVGVFVSGQARLLFAATLVIAILWAVLIVVTF
                     FTQDAFQPDLFSWLSRLSADLIVLLSLWLVNRKYA"
     gene            6915..>7563
                     /locus_tag="VFH83_01440"
     CDS             6915..>7563
                     /locus_tag="VFH83_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6485049.1"
                     /translation="MTFLAVALILAISLPVAADVLRYSYHAGDQYRLVDHVRETVAIN
                     GQYSHRSEILDRIAVTVNQVAADGMSASHSAQFQTSERAWGSQGSYAWEEDYDSEFVR
                     DGQGHYTIDPSYWMPVVRDVPLFPDHSISPGDTWSAPGYEIHDLRQSFGMPDPFRFPI
                     TVSYQWLRSEKRDGIDCAVIGISYDVFYKTGGKPRTTNTYPTRVTGTSQQTLWWDL"
BASE COUNT         1383 a         2438 c         2437 g         1305 t
ORIGIN      
        1 tacgaaccgg gtcttgcgga ccgcagcaga agcgatccgc aagaccccgc agcggcgggc
       61 tggcctactt tgtcttcgcc atcttcatga gcaggtctac cgtccgggag gagtagcccc
      121 actcgttgtc gtaccaggac accaccttga agaatcgctt ctcgcccttg aggttgttct
      181 ggagggtggc caggctgtca tagatcgagg agcggttgtc gtggatgaaa tccgtggaaa
      241 ccacctcctc atccgcaaca ccgaggatcc ccttcaggta ggtctgggag gccttcttca
      301 gcagggagtc gatctcctcg atggaagtgt cccgcgccga acggaacgtc agatccacca
      361 cggagacatc cggggtgggg atacggaagg acatgcccgt gagcttgccc ttgaccgccg
      421 gcaggacctc tcccaccgcc ttggctgcgc ccgtggtgga ggggatgatg ttgatggcag
      481 ccgcccgtcc accgcgccaa tccttcttgg aggggccgtc caccgtcttc tgggtggccg
      541 tgtaggagtg gatggtggtc atgaggccgg tctcgatccc gatcccttcc ttcaggatga
      601 cgtggaccag gggagccagg cagttggtgg tgcaggacgc gttggagacg atatcatgct
      661 tcttgggatc gtactcgttg tcgttgacgc ccatgagcaa ggtcttcacc tcgcccttgc
      721 caggcgccga aatgatgacc ttcctggcgc ccgccgccag gtggcctttg gccttgtcgg
      781 actccgtgaa gaggccggtc gactcgatca cgtactccac acccaggtcc ttccaaggaa
      841 gctgcgccgg ctccttggtg gccaggatgc acttcgtctt gttgccgttc acgatgagga
      901 tgtcatcctc ggctgccccg ggagacttct ccgtagccag ggtcccctgg aaccggccgt
      961 ggaccgagtc gtacttcagc tggtaggcga agtacttggc ttccgtggtg atgtccacca
     1021 cggccaccac gtcgatctcc ttgccgagca atcccttgtc gaccatggcc aggaacacct
     1081 gtcggccgat ccggccgaac ccgttgatcc ctacctttac cgccatatcg cgtctctcct
     1141 tccgaagtcg ttgttctggt tgcggtgact atagccgatt cgaacctttt gtagaaccgg
     1201 gcgccgtctt ttacgcttct gccgcgtccc ggcccgtccg gagcgccttg ggagctccgc
     1261 cggtcacggc ctcaggaatc ctccagcagc cgatagacgg cgtccgcgtc cgtggccttg
     1321 atgatggact ggcgcttgta cttgtcccgg atgatcagcg tgagcttggc caggatccgc
     1381 agatagttct cgtgctcctc gggacccgcg atcaggaagg cgaggtgcac cggcttctcg
     1441 tccagagact gccactccac gccgtctcga aagaccccga tggagatgaa gaaatcccgt
     1501 acccaccggc tcttgatgtg cgggatggcg acattttgac caatgccggt ggtcacgatg
     1561 tattcccgat cgagcaccgc gttctggaac tgctcgaagt cgatcacgtg acgcggatcg
     1621 tcggagagcg cgatgagctt ggccagggcg tcctgcttgt ctctcgcctc cagaaacgcg
     1681 atccgttcct tggcaatgta gtctttcaga ttgatcatgg cttacttcaa tctacaggag
     1741 gattgccccc cgtgcaagga gcgagcgtcg cgatgcgtgg cacgtcacgc gcgtcgcgat
     1801 gcgtcgcgcg tcgtgcagcc gggggctccg ttcggtcagg acccctgact cggaaggatg
     1861 tcctcccact cgaccccttc gccgtccgga atgagccgca tcgcgtgggc tcctatcacg
     1921 cggggaagca gctccggagg gaggcccggt cgcagcacct tctcggtgcg gagcagcgcc
     1981 acgtcctggg agcggatcac ggctccctgc gggatctcgc ggagcgcgtg gatggatcgg
     2041 ttggtacggc ggtagttctc ccgttccgcc gcagccagcg tcttgatgcc cgagcccagc
     2101 acggcgtcta cccgctgccg accgtagcgc gcgccgagcc cggcaatcgc ggcttccggc
     2161 cccaggcgct ccgcggcccg gaccgcggcc accatgcggg agaagtctct gggttcgagc
     2221 gcgatggggt catccagtcc ggagccttcc cgagacagcg tgaaatgctt ctccacggca
     2281 acagcgccgc aggccgccgc ggcggtgggg acgagctctg gatccagcga gtgatccgac
     2341 aggcctacct ctactccgaa cacggcgcgc aggcttcgga ggaccgagac gttgtattgc
     2401 tcctcggggg ccggatacgc ggtgatgcag tgcaagagca cgacgttgtt ccgacccgtg
     2461 atggagagtg cctgctcgat gtcggccagc gtggagaccc ccgaggacag gaagagaggg
     2521 acaccgaagg cggaaacctc ttgcagaagc ggcagatggt tcagctcggg ggaggcgacc
     2581 ttgatcgctt ctgcccccag ccggtggagc tcgcgcgcgc tttggatgcc aaagggggag
     2641 cagagaaaga gcgctccgcg gctctcggtg tgggtcttca gcgcagcgta gaagctgagg
     2701 tcccgctcca gctcccgaaa gcgctcgaac agcgggatcc tcccggtcgg caggtccacc
     2761 agaccggatc tcgggtggag gagctccgtg gcgtagacga gctggaactt cacgcagtct
     2821 gcgcccgcgt ccaccgcggc atcgatcatc gagcgggcgc gggataggtc gccctggtgc
     2881 gacgtaccca gctccgcgat gatcagtgcc ggagcgccgg agccgaccac tcggtcgcca
     2941 agacggatca tcctgccgct cccgcggatt ggtcctgcgc gggaatccga ccgatcatgc
     3001 tcaaggacgc gaccaggatt cccagcggca gcaccgtgag catcacgaag ccccatcgca
     3061 gacccgcggc ctgggagacg gccgccagga gaaaggggaa gctgaaggag ccgatgccgg
     3121 ccgaggtgct taccatgccg accgcggcgc tggatcggaa cgtccgcccg gcgatggcca
     3181 tcacgatggg gtaaagcccc gagtagccga gaccgagcgc gaagatcatg accacggcgg
     3241 gtagaagcga tcgggaagcc aggaaaccag ccagagcggc cacgcagacc accgccagac
     3301 cagccagcag gagctcctgc ctgcggacct gccgcgctcg ggaaaggatg gtccgtccga
     3361 tcatcatccc accccagaga acggacacac ccaaagcgct caccggcccc gaggctccga
     3421 tgacccggac caggtactcc gccacccagt tcgtggcccc cagctcagag ccaacgtaga
     3481 ggatgatcac cacgcacagg aggagaagga ccggctggcg gagcagcgcc acgccgcccg
     3541 cggcgccatg atgtttgccg cgctcgggcg ccgggaaccg ggtggcaagc gcccagaggg
     3601 cgaggatgac gaagaggacc ccgcaagccg gaaaggcgat cttccaggac agtccgctct
     3661 gcatcagggc gccaaccccg atcgggccca cgatggctcc cgcgctgaag ccggcgtgga
     3721 gcaggttcat gagccgactc tcccccggtt tctcgattcg gaccacctcg tactgcgtgg
     3781 cgacctcgat gacgccctga cccgcccccg agacgatgca gacgacgatg ttgacccaag
     3841 gggaggcgga ccttccgaac agcgagagcg ccaccgcggt gccgatcacg gcgacgacat
     3901 agacgccctt cgggttggtg ccctccagga ggaggcccac ggcgaacgtg gaaaagaaga
     3961 agccgatcga cgtcgccgcg agcacgagtc ccgtggtggc gtagctccac cccaggtcct
     4021 ggatgatccg tggtaccgtg gctccgaacg tggtgaggac cgctccgtag accgcgaagc
     4081 cgcaccagag caggatgaac agccatcggt gcgcgggggt catcttctgg gttgtgctca
     4141 tgccgagcct tcggtgtccc ggtcgatccg gctccccacg gttcccctct ggccctggta
     4201 tttgccggca tacggtcgtc ccgcgggctg gtccatcagc gcaaacacga tctggcagat
     4261 ccgacgccca caggccaggc ggatgggcag ccggttggcg ttgtacagct ccagggtgat
     4321 ggtcccctcg aagccggggt ccacccaccc cgcattctgg atgaaaagcc ccatgcggcc
     4381 gatcgagctt cggccctcca cgaacgccgt cacgttggtg ggtatcttga cgtactccac
     4441 cgtggtggcc agcaggaagg aatggggcgg gatgacgatc tcctccttgt cgatccgcac
     4501 gtacggcggc tcccgatcca ggctcaggct ctccagggtg ttttcgtcga tcttcaggaa
     4561 gtgattgccc agccgcaggt caacggaggc cggctggagc tggtacggct cgatcggctc
     4621 gacgatgata ctgcgctccg ccatcatccg gcgaagagtc ccatctgata ggatcatgaa
     4681 ccttctcctt gggtcaggtg ctgccgaatg agcccggcct tcagctcgga gagacgggaa
     4741 gacagagaga ccttgtagtc gtgcgggttg atcagccgct tgtaggtctc atccagcgct
     4801 tgcaggtgcc gcagggttcg ggcctgcagg tccggcagcg agggaagctc cccgacgatc
     4861 tcccctgccg tcatccagcg cttcaggagc gccgtcatgc ggccgtactc cctcagcgtg
     4921 aacgaacgat agtcgccatg cgggtgatga aaggtgtagg ggcgaccagt ctccagcgtc
     4981 tcgtcctcca ggcagatcag gtccgccagg ggcgagccct ccggcgtgac gaaccgccag
     5041 acctgcttta cgccgggatt ggtgaccttc tcgggattgt tggacacctt gatggtgggc
     5101 tcgaagatac cgtcgcgttc ccgctccctg gccgccagct tgtagactcc cgtgagcgcc
     5161 ggatctccgg ctccggtgac cagattagtc cccacgcccc agacgtcgat gggcgctccc
     5221 cgggtgatga gctgatggat gatggcctcg tccagatcgt tggaggccac gatcagggca
     5281 tccgcgagtc cttcggcatc cagacgggcc cgaacccgct tgctgaggta ctcgaggtct
     5341 ccgctgtcca accgtacccc gaagccccgg tggccggccg ccttcagccg cctgccaacg
     5401 gcgaccgcgt tctggaggcc gcttcccagc gtgtcgtagg tatcgatcag gaggagcgcg
     5461 ttgtcggggt acgcgtcggc gtagcgctcg aaagcctccc gctcgttctc gaagaccatg
     5521 acccaggaat gcgccatggt gcctcgcacc gggatcgaaa agcgcttgcc ggccagggtg
     5581 ttggaggtag cggacactcc gccgatgaat gcggcacggg aggccgaaag ggcgccatcg
     5641 atgccctggg cccgacgcaa cccgaactcg atcaccgaac gcccgttcgc cgccaggcgc
     5701 acccgcgcgg ccttggtggc gatgagggtc tggaagttca cgatggcaag gagggcgctc
     5761 tcgaggagct gcgcctcgat cagcgtcgcg tgcacgcgca ggaggggctc ccccgggaag
     5821 gcgagggttc cctcctccat tgcccagatg tcgccccgga accgcatccc ctccaggtaa
     5881 tggaggaact ccggccggaa ggtccccaac ccccgcagat acgcgatgtc ttcctccgtg
     5941 aagcggatat cctgcacgaa ccgtagcgcc gtctccaggc cggcgaagac ggagaagccg
     6001 ccgccaaacg gggctcgccg gaagaagaga tcgaacacca cgtgagggtt cctctggtga
     6061 aggaagtacc cctgcatcat ggatagctcg tagaaatccg tgagaagggt caggctggga
     6121 tcacgttccc gtgctgtcat ccgatgggaa tggaggatgg gggattcgaa cccccgacct
     6181 acagattgcg aacctgccgc tctaccaact gagctaatcc cccgaggagg gcccaggcgc
     6241 cccgcacccg ggcaacagta gccaccggga atgacgaggt caagggccgg acggaccggg
     6301 tggctgcccg gaagtgtttc cggggcgggc tgccggcgac cggcagcccg cctcgcgcct
     6361 caggcgtact tgcggttgac cagccagagg ctcagaagga cgatgaggtc cgcgctgagt
     6421 cggctgagcc aggagaagag gtccggctgg aatgcatcct gggtgaagaa ggtgaccacg
     6481 atcaacaccg cccagaggat cgcgatcacc agggtcgccg cgaagagcag gcgagcctgc
     6541 ccagagacga atacgcccac aaagacgatc acgcccgccg cgagctcgca gatggcgacg
     6601 atcaggttga atggattgtt cgttccgccg aagaagcgcg tgagtcctcg gctgaactga
     6661 ctcgcgttgg agttccagtc gttgatcccg acaacgccga ggctgatcag gaataccgcc
     6721 agaacgatct ggatcaccac caccacgtcg aaggagactc tcttccttgc catggtagga
     6781 agcttacctc gcggaaggct ccgctgcatc ccagctctcc ggtccagggc ttgccggttt
     6841 cttccgataa tgtgggtaga ctaaccttac gcggcgcagg gaggagcgac atgcggcgtc
     6901 taccaggagc ttccgtgaca tttctggccg tggccctcat tctggctatc tcgttgcccg
     6961 tcgctgcaga cgtgcttcgc tactcctacc atgccgggga ccagtatcgg ctggtggatc
     7021 acgttcggga aaccgtggca atcaacggcc agtactccca tcgatccgag atcctcgatc
     7081 ggatcgccgt gacggtgaac caggtagccg ctgacgggat gagcgccagc cacagcgcgc
     7141 aattccagac ctccgagcgg gcgtggggat cccagggcag ttacgcctgg gaggaagact
     7201 atgactcgga gttcgtgcgc gatggtcagg gacactacac gatcgaccct tcctactgga
     7261 tgccggtggt tcgagacgtg ccgctcttcc ccgaccacag catctctccg ggagacacct
     7321 ggtccgcccc cggctacgag atccacgacc tgcgccagag ctttgggatg cccgacccct
     7381 tccggttccc catcacggta agctaccagt ggctgcggtc ggagaagcgg gacggaatcg
     7441 actgcgccgt gatcgggatc tcctacgatg tgttctacaa gacgggtggg aaaccgcgga
     7501 ccacgaacac gtatcccacg cgggtgaccg gcacttccca gcagactctc tggtgggatc
     7561 tcg
//
LOCUS       DASRZS010000059         3681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5422087, whole genome shotgun sequence.
ACCESSION   DASRZS010000059 DASRZS010000000
VERSION     DASRZS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5422087"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFH83_01445"
     CDS             complement(<1..269)
                     /locus_tag="VFH83_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013056854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET6485050.1"
                     /translation="MRIGVFSVLFQDQPFEKAMDKVAAAGLTAVEIGTGGYPGNHHCP
                     MDELLESPAKRKSYRAAVEGRGLIISALSCQWEPLSPDARLAKDS"
     gene            complement(362..1525)
                     /locus_tag="VFH83_01450"
     CDS             complement(362..1525)
                     /locus_tag="VFH83_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6485051.1"
                     /translation="MESWERRLRMGMVGGGQGAFIGWVHRMAANLDGQIDFTAGCFSR
                     DPENTRITGRQLYLDPSRTYSTWQEMAEKEARLPAEKRIDFVSIVTQTVSHFEIAKAF
                     LERGIHVVLDKPMTYDLAQAEELVRIVERTGLVFCLTHNYTGHPLIRHARELFRSGQM
                     GKVRKVLVEYLQDFLMYPHEKEGMKQAVWRTDPAQAGAVGTLGDAGSHCENLLEYITG
                     ETVSELCADFTTFLPDRVLEEDASLLLRLSGGGRGAMTVSQIAVGEENGLTIRIYGEK
                     GGILWAQENPNYMDIYRYNQPRQTITRSHKEYLNDFATWSTRVPTGHPEGYLEAFANL
                     YCGAVEAIRRYIDGKPMKTLEYKFPTVYDGLRGMRFVTRAGESAKRGSVWVKV"
     gene            complement(1542..2288)
                     /locus_tag="VFH83_01455"
     CDS             complement(1542..2288)
                     /locus_tag="VFH83_01455"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6485052.1"
                     /translation="MASDTLNPNSKLPLYHQLYELFQDKIRAHQWKPGDMIPPESELI
                     ERYRVSRITVRKVLDMLVKEGHIQRERGRGTFVAQPKLEHGMTRIISFTEDMRQRGFR
                     PSTRILFSGMVAAPANIAQALRVAEGEELARIDRLRLADEEPMCVEESFLVHRHMPGI
                     LDHDLTVNSLREVKQRVYGIRWSRAIQTIQAMAATPEIAHLLCIKAGAPLLYFERVTF
                     SQDDIPMEYLRIYYRADRYVLHNELVGGSG"
     gene            complement(2326..3495)
                     /locus_tag="VFH83_01460"
     CDS             complement(2326..3495)
                     /locus_tag="VFH83_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET6485053.1"
                     /translation="MAKPRYTKKVVGVLENPGGPGPMNTLLVVGDVNSDGRPDIVLSG
                     RNGNLAWFENRGQGKPWERHPIDDITNLECGGCLYDLMGKGRPDLINGSDYGSVDLLW
                     WENPGAAGGKWVKRIVAPTGNRQFHDTLIGDVTGDGSLSLVFTNQHGRGGTWVYRVPL
                     PQDPRQSPWPGLEVIVDGITEVNPGRPEGVQPEEGVAIGDVDGDGKNEVVAGTHWYKK
                     VKGAWQRHKFASGYITTKIQIADIDGDGKNEIVLSEGDPCVYGRTRGGKAGWFKPKGD
                     LTALWEEHVLEDNLLDAHSLRVADLCGNGRADILVGEVGFADHATDAYVGKQPQLLIF
                     ENTGKGEFTRHLIDEGTGCHDAWLADLYGRRVLDIVTRPLHGPEKWNLHVYERER"
BASE COUNT          577 a         1250 c         1140 g          714 t
ORIGIN      
        1 tcggagtcct tggccagacg ggcgtccgga ctgagaggct cccactggca gctcaaggcg
       61 ctgatgatga ggccccggcc ttccacggcg gctcgatagc tcttgcgttt cgccgggctc
      121 tccagaagct cgtccatggg acagtgatgg ttgccggggt agccgcctgt accgatctca
      181 acggcggtaa ggccggcggc cgccaccttg tccatggcct tttcgaaggg ctggtcctgg
      241 aacaggacgc tgaaaactcc gatccgcatg ggtgcctccc cttgggcgaa cgccctggcg
      301 tcgcgacaag ccgtatcccc ggcagcgggc gggatagcgc tcccgggcgc tcacctgccg
      361 gctacacctt cacccacacc gacccgcgct ttgcggactc cccggcccgc gtcacgaacc
      421 gcatgccgcg aagcccgtcg tacaccgtgg gaaacttgta ctccaaggtc ttcatgggct
      481 tgccgtcgat gtagcgccgg atggcctcca cggctccgca gtagaggttg gcgaatgcct
      541 ccaggtagcc ctcgggatgt ccggtgggga cccgggtgct ccacgtggcg aaatcgttca
      601 ggtactcctt atgggagcgc gtgatggtct ggcgaggctg gttgtagcgg tagatgtcca
      661 tgtagttggg attctcctgc gcccacagga tcccgccctt ttcaccgtag atccggatgg
      721 tgagcccgtt ctcctctccc acggcgatct ggctcacggt catggcgccc cggccgccgc
      781 cgctcagtcg caggagcaga ctggcatcct cttccagcac ccggtccggc aggaacgtcg
      841 tgaaatccgc gcagagctca ctcaccgtct cgcccgtgat gtattccagc aggttctcgc
      901 agtgcgagcc ggcatccccc agggtgccga ccgcgccggc ctgcgcgggg tccgtccgcc
      961 agaccgcctg cttcatccct tccttctcgt gcgggtacat gaggaaatcc tggaggtact
     1021 ccaccaggac cttgcgaacc ttgcccatct ggccggagcg gaacagctcc cgcgcgtggc
     1081 gaatcaaggg gtggccggtg tagttgtgcg tgagacagaa gacgaggccg gtccgttcga
     1141 cgatgcggac cagttcctcc gcctgggcaa ggtcgtaggt catgggcttg tccaggacca
     1201 cgtggattcc gcgctccagg aacgccttgg cgatttcgaa atgggagacg gtctgggtga
     1261 cgatggagac gaagtcgatc cgcttctctg caggcagacg cgcctccttc tccgccatct
     1321 cctgccacgt ggagtacgtc cggctcgggt caaggtagag ttgccgcccg gtgatccggg
     1381 tgttctcagg gtcccgggag aaacagcccg ccgtgaagtc gatctggccg tccagattgg
     1441 cggccatgcg atgcacccat ccgatgaagg cgccctggcc gcctcccacc atccccatgc
     1501 gaagccttcg ctcccagctc tccatggttt cccccggtgc gctatcccga gccgcccacc
     1561 agctcgttgt gcaggacgta ccgatccgcg cggtaataga tacgcaggta ttccatgggg
     1621 atgtcgtcct gggagaaggt gacgcgctcg aagtacagaa gcggggctcc cgccttgatg
     1681 cacaggaggt gggcgatctc aggcgtggcc gccatggcct ggatggtctg gatggcgcgc
     1741 gaccagcgga tcccgtacac gcgctgcttc acctcacgca gggagttcac ggtgaggtca
     1801 tggtccagga ttcccggcat gtgtcggtgg accaggaagg attcctcgac gcacatcggc
     1861 tcctcgtccg cgagccgcag ccggtcgatg cgggccagct cctccccttc ggcgacccgc
     1921 agggcctggg cgatgttggc cggcgccgcg accatgccgg agaagaggat ccgcgtgctg
     1981 ggccggaacc cgcgctgacg catgtcctcg gtgaagctga tgatgcgggt catgccgtgc
     2041 tcgagcttgg gctgggcgac gaaggtgccg cggcctcgct cccgctgaat gtggccttcc
     2101 ttcacgagca tatccagcac tttgcgcacc gtgatccggc tcacccggta gcgctcgatc
     2161 agctccgact ccggcgggat catatccccc ggtttccact ggtgcgcgcg gatcttgtcc
     2221 tggaacagct cgtagagctg gtgatagaga ggaagcttgc tgttggggtt cagcgtatca
     2281 ctcgccatgg cccctccatg ggaacggctg tccgccgctc tgccatcagc gctcgcgctc
     2341 gtagacgtgg agattccatt tctcgggtcc gtggagcggt cgggtgacga tgtccagcac
     2401 cctgcggccg tagaggtccg cgagccacgc gtcgtggcag ccggtcccct cgtcgatgag
     2461 gtgccgcgtg aactcaccct tgcccgtatt ctcgaagatg agaagctgcg gctgcttgcc
     2521 cacgtaggcg tccgtggcgt ggtccgcgaa ccccacttct cccacgagga tatccgcccg
     2581 tccattgccg cagaggtcgg ctaccctcag ggaatgggcg tccaacaggt tgtcctccag
     2641 gacgtgctct tcccagagcg cggtgaggtc gcccttcggc ttgaaccatc cggccttccc
     2701 gccccgagtc cggccgtaca cgcaggggtc tccctccgag aggacgatct cgttcttccc
     2761 gtctccatcg atatccgcga tctggatctt ggtcgtgatg tatcccgagg cgaacttgtg
     2821 gcgctgccag gcgcccttca ccttcttgta ccagtgggtg ccggcaacga cctcgttctt
     2881 cccatcgcca tccacgtcgc cgatggcaac gccctcttcg ggctgaacgc cctcgggccg
     2941 gcccgggttc acctccgtga tcccgtccac gatgacttcc agccccggcc acgggctctg
     3001 ccggggatcc tggggcagcg gaacgcgata gacccaggtc cctcccctgc catgctggtt
     3061 ggtgaagacc agggacagag acccatcccc ggtcacgtct ccgatcaggg tgtcgtggaa
     3121 ctggcggttg ccggtaggag ccacgattcg cttcacccac ttgcctccgg cggcacccgg
     3181 gttctcccac cacaggaggt ccaccgatcc gtagtcgctc ccgttgatga gatcgggtct
     3241 gcccttcccc atcaagtcat agagacagcc gccgcattcc aggttggtga tgtcgtcgat
     3301 gggatgcctc tcccagggct tgccctggcc ccggttctcg aaccacgcca ggttgccgtt
     3361 ccgccctgag aggacgatgt ccggcctgcc atcggagttc acgtctccaa ccacaagaag
     3421 ggtattcatg ggtcccgggc cacccggatt ctccaggact cccacgacct ttttcgtgta
     3481 cctcggcttc gccacgctct cccccaatcc ccgtcgatcc ctgacgcccg ggccgcaagg
     3541 cctggcgcgt tcggcaacct gtcagaccaa ggccctgggc tcgcttccta tccctgctga
     3601 gtggagccct ccgctggacc gacataatgt gctgacatta tgacgagtct atagcgaccc
     3661 gatggcaccg tcaagagccg c
//
LOCUS       DASRZS010000060         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1961098, whole genome shotgun sequence.
ACCESSION   DASRZS010000060 DASRZS010000000
VERSION     DASRZS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1961098"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="VFH83_01465"
     CDS             <1..1169
                     /locus_tag="VFH83_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6485054.1"
                     /translation="LERRAEREEVRQKDEQRRATILRRELEWLKRGPRARTGKDKGRK
                     QRIQELSDTRAPAQTAMQELTSAHRRQGKKVLEMLAVTKSYDGRQVLRPFTHSFRRGE
                     RIGLIGPNGSGKTTFLKLVAGEVAPDTGSAIRGETTVFAYLPQAGGEGRKNVTVLDFM
                     KELGERIPQERGGSLSAEQFLERFLFPRAMHAVTLDRLSGGELRRLTLVRLLATAPNF
                     LLLDEPTNDLDLDTIRLLEDYLTDFAGCILLVSHDRALLDRLTDALLVFDGAGGARHF
                     TGSYEEYRLTPGSDAEGPAAEPRREPSPPRESSRSPRVGRSRLSFKERKEYEDLLGEI
                     ETLEREQKALEESFQQQPIDPDVRVRDTRRYQEVARTIELRMARWEELAGRAGD"
     gene            1201..>2208
                     /locus_tag="VFH83_01470"
     CDS             1201..>2208
                     /locus_tag="VFH83_01470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HET6485055.1"
                     /translation="MGKPVEPDAKDLARALKQHFGFAGFRPHQSEIVQAVLRGKDVFA
                     SLPTGGGKSLCYQLPAVMRDGLTLVVSPLISLMKDQVDGAREDGIPAAFLNSTLSSDE
                     SRETWRELASGSIRLLYASPERLSIPAFRASLARFGLALIAVDEAHCISEWGHEFRPD
                     YRSLGLLRAEFPEVPIAAFTATATPHVQDDVIQLLGLKSPFVVRASFDRPEIFYRVRS
                     REGDGDAQVLQFIRRHGGEPGIVYRGTRKAVERTAEFLTAAGEDAVAYHAGLEDKLRR
                     SRQERFRKDQVMVVVATIAFGMGIDKPNVRWVVHADLPRSIEGYYQETGRAARDGEDA
                     QA"
BASE COUNT          390 a          704 c          764 g          350 t
ORIGIN      
        1 acctggagcg aagggcggag cgcgaggaag tgcgccagaa ggacgagcag cgccgggcaa
       61 ccatcctgag gcgcgagctg gaatggctga agcgggggcc ccgggcccgc acgggcaagg
      121 acaagggccg caaacagcgt atccaggagc tttcggatac ccgcgcgccg gcgcagacgg
      181 ccatgcagga gcttacctcc gcccatcgcc gccaggggaa aaaggtcctg gaaatgctgg
      241 ccgtcaccaa gagttacgac ggtcggcagg tcctgcggcc cttcacccac agctttcgcc
      301 gtggggagcg catcggcctc atcggcccca acggatccgg gaagaccacg tttctcaagc
      361 tggtggccgg ggaggtggcc ccggacaccg gaagcgcgat ccggggggag acaacggtat
      421 tcgcctatct tccccaggcg ggcggtgagg gaagaaagaa cgtcaccgtc ctcgatttca
      481 tgaaggagtt gggcgagcgt atcccccagg aacggggcgg ctcgctctcc gccgaacagt
      541 tcctggagcg gttcctcttt ccccgcgcca tgcatgccgt cacgctggat cgactctccg
      601 gcggagagct gcgacggctc acgctcgtgc ggcttctggc cacggctccc aacttcctgc
      661 tgctggacga gccgaccaac gacctggacc tcgatacgat ccgcctgttg gaggattacc
      721 tgacggactt cgccggctgc atcctcctgg tctcccatga ccgggccctt ctcgaccggc
      781 tgaccgacgc cctcctggta ttcgacggcg cggggggagc caggcacttc accggtagct
      841 atgaggagta ccgcttgacg ccaggctccg acgccgaagg tccggcggcg gagccgcggc
      901 gcgagccgtc gccgccacgc gaatcgtcgc ggtcgccgcg cgtcggcaga tcgcggctgt
      961 ccttcaagga gcgcaaggag tacgaggatc tccttgggga gatcgagacg ctcgagaggg
     1021 aacagaaggc gctggaggag tccttccagc agcaaccgat cgatcccgat gtcagggtca
     1081 gggacaccag gcgatatcag gaggtcgcgc gaaccatcga gctgcggatg gccaggtggg
     1141 aagagctggc cggccgcgcc ggtgattgag cgcggagact gagcgcggag ggtgggcgcc
     1201 ttggggaaac cggtggaacc ggacgcaaag gatctggcac gggcgctgaa gcagcacttt
     1261 gggttcgccg ggtttcggcc ccaccagagc gagatcgtgc aggccgtcct gcgcgggaag
     1321 gatgtgtttg cctctctgcc cacgggcgga ggaaagtcgc tctgctacca gcttcctgct
     1381 gtcatgcggg acggtctcac gctcgtggtg agccccctga tctcgctcat gaaagaccag
     1441 gtggacggcg ctcgcgaaga cggcatcccc gccgcgtttc tcaacagcac cctctcctcg
     1501 gatgagtccc gggagacgtg gagggagctc gcctccggct ccattcgcct gctctacgca
     1561 tcccccgagc gactgtccat ccccgccttc cgcgcgtccc tggcccggtt cgggttggcg
     1621 ctgatcgccg tggatgaggc acactgcatc tccgaatggg ggcacgagtt ccgtcccgac
     1681 taccgttctc tgggtcttct tcgcgctgag ttccccgagg tcccgattgc cgcattcacc
     1741 gcaaccgcga cgccgcacgt gcaggatgac gtgatccagc ttctggggct gaagagcccg
     1801 ttcgtggtac gcgcaagctt tgacagaccc gagatcttct accgggttcg gtcccgggag
     1861 ggtgacggtg acgcacaggt cctgcagttc atccggcgac acggcgggga gccggggatc
     1921 gtataccgcg gcacgcggaa agcggtggag cgcaccgcgg agttcctcac cgcggcgggg
     1981 gaagacgccg tcgcgtacca cgcgggcctg gaggacaagc tgcggcgttc ccggcaggag
     2041 aggttccgaa aggaccaggt gatggtggtc gtggccacca tcgcgttcgg catgggcatc
     2101 gacaagccaa acgttcgttg ggtggtgcac gcggatcttc cccgcagtat cgaaggctac
     2161 taccaggaaa cgggcagggc ggcccgcgac ggtgaggacg cgcaagca
//
LOCUS       DASRZS010000061         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3115756, whole genome shotgun sequence.
ACCESSION   DASRZS010000061 DASRZS010000000
VERSION     DASRZS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3115756"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFH83_01475"
     CDS             complement(<1..196)
                     /locus_tag="VFH83_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485056.1"
                     /translation="MITRGVLAAFLSATGLGDSVTACVESYQTADRSEREHRFGAICR
                     SVMDAAIPEGLRVGVEHAALA"
     gene            402..737
                     /locus_tag="VFH83_01480"
     CDS             402..737
                     /locus_tag="VFH83_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6485057.1"
                     /translation="MDVSEVLKALADPNRLRILNLLEKRTLCVCDLEAILELNQSNLS
                     RHLAKLKQAGIVSAQKKALFTYYSRRELPEPYGPIVDALCAAIHDDPVWEPDRARLEK
                     RLRTSKNRC"
     gene            801..1592
                     /locus_tag="VFH83_01485"
     CDS             801..1592
                     /locus_tag="VFH83_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017755314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HET6485058.1"
                     /translation="MKHFTKDELRTAVRERYGATARGQGSDCGCSSSCCSPSASSRQT
                     SQALGYTAEEMEAAPEGSNLGLGCGNPQAIASLRPGETVLDLGSGGGFDCFLAARAVG
                     PQGKVIGVDMTADMVALSRRNAGKSGRTTVEFRLGEIEHLPVADSSVDVIISNCVINL
                     SPEKPQVFREAFRVLKSGGRLAISDTVASAPIPEELKDDLASYSACVTGSASVDELHG
                     MLRDAGFANIRIRPREESKQFIKEWSPDQGLENFILSASIEAVKP"
     gene            1610..2041
                     /locus_tag="VFH83_01490"
     CDS             1610..2041
                     /locus_tag="VFH83_01490"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HET6485059.1"
                     /translation="MPKVLFVCTHNSARSQMAEAYLRKLGGGAFEVESAGLEPGALNP
                     YVVRALLEEGIDISGHQTNDVFQFFKQGRRYDFVVTVCSSEAAERCPFFPGRSKRLHW
                     SFPDPSTFAGSDQEIMSQVREVRDAIREKILGFVEELDGAR"
     gene            2046..3104
                     /gene="arsB"
                     /locus_tag="VFH83_01495"
     CDS             2046..3104
                     /gene="arsB"
                     /locus_tag="VFH83_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HET6485060.1"
                     /translation="MREGSGRASPGGRLSVLDRWLTLWIFLAMAAGVLLGYLIPGVEP
                     FINRFQLGTTNIPIAIGLILMMYPPLAKVRHEELGDVFRNLRVLGLSLLQNWIIGPIL
                     MFALAILFLRDHPAYMTGLILIGLARCIAMVIVWNDLARGDTEYAAGLVAFNSIFQVL
                     FYSVYAWLFVTVLLPVFGLPGTTVAVTLGQIAKSVFIYLGVPFLAGFVTRLALVRARG
                     KEWYHRRFVPRIGPITLVALLFTIVVMFSLKGALIVQIPLDVLRIAVPLLIYFAVMFL
                     LSFFLSRALGTGYARTATLSFTAGSNNFELAIAVAVFGIGSGVAFAAVIGPLVEVPVM
                     IGLVRVSLAFRGRLFGLA"
     gene            complement(3135..>3603)
                     /locus_tag="VFH83_01500"
     CDS             complement(3135..>3603)
                     /locus_tag="VFH83_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET6485061.1"
                     /translation="TTSIVSETGVSPSGLIFELTESAAVKDIDHTRKTMGRLAELGVR
                     FIIHDFGTSYASLRWLKTVPIYAIKLDKFFVQNIAGDPDSLAIMRAIISMAHTLNVEV
                     IAEGIETKEQAERLKAMDWRDMPSARCNQVQGFFIARPMTPEDLAAMARPSVP"
BASE COUNT          609 a         1190 c         1119 g          685 t
ORIGIN      
        1 cagcgagggc ggcgtgctcc acgcccacgc gaaggccctc ggggatggcg gcgtccatga
       61 cgcttcggca gatcgctccg aaccggtgct cccgctcgga gcggtccgcg gtctggtagc
      121 tttcaacaca cgccgttacc gaatcgccga gtccggtcgc gctcagaaag gcagcgagga
      181 ccccgcgggt gatcacaacc catccggcac gcgaacgccc atcgacacgg cacgcgcgag
      241 gtttgccgcc ttgccgccga cgcgcgtccc gtcccgggct tccgaaagcc ttatggtctc
      301 catggaagtc tgcggcgaat ctacgacctg cggtcatccg cgtcaaaccc agagggagcc
      361 ccaccatacc cactcccggg gctttccatt actctatgat catggacgtc tccgaggttc
      421 tcaaggcgct tgccgatcca aaccgactgc ggatcctcaa tctgctggag aaacggaccc
      481 tgtgcgtctg tgacctggaa gccatcctgg agctcaacca gtccaacctg agccgccacc
      541 tcgccaagct caagcaggcg gggatcgtga gcgcccagaa gaaggccctg ttcacctact
      601 actcccgcag agagctgccc gaaccctatg gccccatcgt ggacgcactc tgcgcggcca
      661 tccacgatga cccggtctgg gaaccggacc gggcccggct ggagaaacga ctccgcacat
      721 cgaagaaccg ctgctgaccc gttgacaccc gtcattcatt actgtatact catatagtaa
      781 tctagacagg gaggttccgg atgaagcact tcaccaagga tgagctgcgc actgcggtcc
      841 gagagcgcta cggggccacc gcgcgcggtc agggctccga ctgcggatgc tcctcctcct
      901 gctgttcccc gtccgcgtcc tcccggcaga cgtcccaggc cctcggctac acggcggagg
      961 agatggaggc cgcgcccgag ggatcgaacc ttgggctggg ctgcgggaac ccacaggcca
     1021 tcgcttcgct gaggccgggc gagaccgttc tggatctggg gagcggaggc ggattcgact
     1081 gtttcctggc ggcacgggcg gtaggacctc agggcaaggt gatcggcgtg gacatgacgg
     1141 cggacatggt cgcgctgtcc cgcaggaatg ccgggaagtc cgggcgcacc accgtggagt
     1201 tccggctggg tgagatcgag cacctaccgg ttgcggattc ctccgtggac gtgatcatct
     1261 ccaactgcgt gatcaacctt tccccggaga agccccaggt gtttcgagag gccttccgcg
     1321 tgctgaaaag cggcggccgt ctggcaatct ctgataccgt ggcgtccgca cccatccccg
     1381 aagagctgaa ggacgacctg gcctcctatt ccgcgtgcgt gaccggctcg gcgtcggtgg
     1441 acgagctgca cggaatgctg cgggacgccg ggttcgcgaa catccggata cggccgcggg
     1501 aggaaagcaa gcagttcatc aaggaatggt ccccggacca ggggctcgag aacttcatcc
     1561 tttcggcctc catcgaggcc gtgaagccgt aaggctcagg agcgcgggca tgcccaaggt
     1621 actgttcgtc tgcactcaca actccgcgag gagccagatg gcggaggcct atctgcgcaa
     1681 gctcggcggc ggcgctttcg aggttgagag cgcgggcctg gagccgggcg ccctgaaccc
     1741 gtacgtggtc cgcgccctgc tggaggaagg catcgacatc tcgggacacc agaccaacga
     1801 cgtgttccag ttcttcaagc agggcagacg ctacgacttt gtcgtaacgg tctgctccag
     1861 cgaggccgcg gagcgctgcc ctttctttcc cgggcgttca aagcggctcc actggtcctt
     1921 cccggatccg tccacgttcg ccggctccga ccaggagatc atgagccagg tgcgggaagt
     1981 gcgagacgcg atccgggaga agatcctggg gttcgtcgag gagctggacg gtgcccgctg
     2041 agaccctgag ggaaggatcc gggagagctt cgccgggggg caggctttcc gttctcgacc
     2101 gctggctgac gctgtggatc ttccttgcca tggcagcagg agtcctgctt ggctacctca
     2161 ttcccggtgt tgagccattc atcaaccgct tccagcttgg caccacaaac atcccgatcg
     2221 ccatcgggct gatcctcatg atgtacccgc ccctcgcgaa ggtccgccat gaggagcttg
     2281 gcgacgtgtt cagaaacctc agggtgctcg gtctctctct tctccagaac tggatcattg
     2341 gcccgatcct gatgttcgcc ctcgccatcc tcttcctccg ggaccacccg gcgtacatga
     2401 cgggcctcat cctgattggc ctggcacgct gcatcgccat ggtcatcgtc tggaacgacc
     2461 ttgcgcgggg cgacacggaa tacgcggccg gactggtggc gttcaacagc atcttccagg
     2521 tgctcttcta cagcgtttac gcatggctct tcgtgacggt cctcctgccg gtgttcgggc
     2581 tgccggggac gacggtggcc gtcaccctgg ggcagatcgc aaagagtgtg ttcatctacc
     2641 tcggggttcc gttcctcgcc ggattcgtca cgcgactcgc tctggtgcgg gccaggggca
     2701 aggagtggta ccaccgccgg ttcgttcccc ggatcggccc catcaccctg gtggcgctgc
     2761 tgttcaccat cgtcgtgatg ttcagcctca aaggcgcgct gatcgtccag atcccgctgg
     2821 acgtgctccg gattgccgtt ccccttctca tttacttcgc cgtcatgttc ctcctgagct
     2881 tcttcctcag tcgggccctg ggcaccggct acgccaggac ggcgacgctt tccttcacgg
     2941 cagggagcaa caacttcgag ctcgccattg cggtggcggt gttcgggatc ggatcggggg
     3001 tggcgttcgc cgccgtcatc ggccccctgg tcgaggtccc ggtcatgatc ggcctggtcc
     3061 gcgtctcgct tgccttccgc gggcgactgt tcggcctggc ctgaaggcgg tccaaccccc
     3121 cgcgggaccc agggctaagg gacggacgga cgcgccatcg ctgccaggtc ttccggcgtc
     3181 atgggccgcg cgataaagaa gccctggacc tgattgcagc gcgcggacgg catgtccctc
     3241 caatccatgg ccttcaacct ttcggcctgc tcctttgtct cgatcccctc cgcgatcacc
     3301 tcgacgttga gcgtatgggc catggagatg atggcccgca tgatcgcaag actgtccggg
     3361 tcaccggcaa tgttctggac aaagaacttg tcgagcttga tggcgtagat cggcacggtc
     3421 ttgagccatc tcagcgacgc atagcttgtc ccgaagtcat ggatgatgaa ccgcacaccc
     3481 agctccgcca gtcgccccat cgtctttcgc gtgtggtcga tgtccttgac ggcggcgctc
     3541 tcggtcagct cgaagatcaa gccggacggg ctgacgcccg tctcgctcac aatcgacgtc
     3601 gtc
//
LOCUS       DASRZS010000062         4278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4271612, whole genome shotgun sequence.
ACCESSION   DASRZS010000062 DASRZS010000000
VERSION     DASRZS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4271612"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFH83_01505"
     CDS             complement(<1..554)
                     /locus_tag="VFH83_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010272793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485062.1"
                     /translation="MSNTAAAPGVPRPRRRGLLVRALRSWQLYLFLLPTLVYFGIFQY
                     GPLYGAQIAFRDYLGALGIWGSPWVGLENFIRFFKSAYFVNLLVNTLSLSLLMLVFSF
                     PIPVFFALMLNQLGRQRYKRIVQTVSYAPHFISVVVFVGMMFLFASPSTGIINILMAK
                     MGGPKIYYMGSLPWFRPVYVLSDI"
     gene            complement(619..2238)
                     /locus_tag="VFH83_01510"
     CDS             complement(619..2238)
                     /locus_tag="VFH83_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485063.1"
                     /translation="MRHWFVFLVLTVLVVPAFAQVTGEEQAAGFHATGYPIVDKTVTL
                     RVFGQKDPLHRKPFDELPVIKETEKLTNVHIEWIEVPAAAMEEKKNLMLASNDLPDAF
                     SENLPDFDIVTYGAAKVFVPLQGLIQKYMPNYQAVIKQKPDIMKFTTAPDGNIYTFAR
                     LNEGSWMRQLSVHNIYKPWVDKLGRKMPATTDEFYDLMVAMKNADLNGNGKADEIPIA
                     MAWGDLPNGVPRTGPWFVYYAFGLPAQDTGSLWLPISNLFVEDGKVRFAPADPRFRDA
                     TAYLNKLWKAGLIEPEAFTLTYPQMEAKARAMPVTYGVLSVWNLQDEFTPGSDPRANE
                     YVPLPMFTAPGVSRPAIYRQPFPGWNRGHMAITRVNKYPNVTARWIDYIYEPYHTMEW
                     IEGMIGSRLVKDDKGVVTMKAPPAGMTTQEYRMSETNPMVPLAATMELYRTLFPAPIY
                     EMKGAVIDRYYAPYWPKEFWTNPLMSPSDMKQLNAIDTDLRALVTKTQAKWITQGGVE
                     TEWDGYLRQLDSLGLKTMLQVRQAAYDRLNK"
     gene            complement(2283..3794)
                     /locus_tag="VFH83_01515"
     CDS             complement(2283..3794)
                     /locus_tag="VFH83_01515"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HET6485064.1"
                     /translation="MRATIKVDADRVLGEVDRLIFGHFIEHLGRCIYGGVLDEHGRYR
                     DEVIAAMRRIRPSILRWPGGCFADGYHWRDGVGPVEERPTRINPAWRNKDETNRFGTD
                     EFVQYCQRLGAQPYICANVGSGTPEEAAAWVEYCNGTGATAYARMRRANGHEEPFGVR
                     YWGVGNEIDETEHVNEIGCLSAEEYSRAVREYSKLMRRASAGITLVGVGSNRGEVDWN
                     LTVLEKAGQYLDYIAQHGYYGTDDHYSTVAGPVHVEKRIRMLQSAIELAAFTTGLTRR
                     IPIAFDEWNVWYRTRKDPQDLFEEVYELKDALFVAGTFNALQRMCGIVRMANLAQMVN
                     ALGMIHADEAGVVLSPLYHAFDLYANHTGTTVFDTFTVSETFDTHDNGGPRGLGPLSG
                     VPYVDASATLREDSGRLLCLAVVNRHRDEPVECRIRLDGFSVKDSAAVFELTGAGPDA
                     RNTVREPEAVAVKPRPAARVGGDFVHTFAARSATVLEIPLTGGAPGPRSREKS"
     gene            3943..>4278
                     /locus_tag="VFH83_01520"
     CDS             3943..>4278
                     /locus_tag="VFH83_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485065.1"
                     /translation="MATLGAAACAALVACSLSTQPPSVRDWLVFGSDVAHSSAPATAG
                     LTRSSVASLHLQQVMLDGTVDGSAVYVHGASVGGSQHNAFFVTTTYGKTIAIDADDGA
                     VLWEYTPASY"
BASE COUNT          709 a         1392 c         1374 g          803 t
ORIGIN      
        1 cagatgtcgg agagcacgta caccggacgg aaccatggca ggctccccat gtagtagatc
       61 ttcgggccgc ccatcttcgc catcaggatg ttgatgatcc cggtggacgg ggaggcgaac
      121 aggaacatca tccccacgaa caccaccacg gagatgaagt gcggcgcgta ggagaccgtc
      181 tgcacgatcc gcttgtacct ctggcggccg agctggttca gcatcagcgc gaagaacacg
      241 gggatcggga agctgaacac gagcatcagg agactcaggg aaagcgtgtt gacgaggagg
      301 ttgacgaaat aggcggactt gaagaagcgg atgaagttct ccagccctac ccaggggctg
      361 ccccagatcc ccagggcccc gaggtagtcc cggaaggcga tctgggcccc gtacaacggc
      421 ccgtactgga agatgccgaa gtacaccagg gtgggcagga ggaagaggta cagctgccag
      481 ctgcgaaggg cccgcaccag gagcccgcga cgtctcggcc gcggaacacc cggtgcggcg
      541 gcggtgttgc tcactctcac ctcagggtcc ggcgccgagt ggcgccgggc ggggttctca
      601 actcggtccc gttcgcgact acttgttcag cctgtcatag gcggcctggc ggacctgcag
      661 catcgttttc agtccgagac tgtccagttg cctgaggtag ccatcccact cggtctcgac
      721 gccgccctgg gtgatccact tcgcctgcgt cttggtcacg agggcccgga ggtccgtgtc
      781 gatggcgttc agctgcttca tgtcggaggg agacatcaac gggttggtcc agaactcctt
      841 gggccagtac ggcgcgtagt atctgtcgat gaccgctccc ttcatctcgt agatcggcgc
      901 cgggaacagc gtccggtaga gctccatggt ggcggccagc ggcaccatgg ggttcgtctc
      961 cgacatccgg tattcctgcg tggtcatgcc ggccggcggc gctttcatgg tcaccacgcc
     1021 cttgtcgtcc ttcacgagcc tgctgccgat catcccctcg atccattcca tggtatggta
     1081 gggctcgtag atgtagtcga tccagcgggc ggtcacgttc ggatacttgt tgaccctcgt
     1141 gatcgccatg tgcccccggt tccaccccgg gaacggctgg cgatagatcg ccggccggga
     1201 gacgccgggc gccgtgaaca tgggaagcgg cacgtactcg ttggcgcgag ggtcgcttcc
     1261 gggcgtgaac tcgtcctgga ggttccacac cgagagcacg ccatatgtca cgggcatggc
     1321 ccgggccttg gcctccatct gcgggtaggt cagggtgaac gcttccggct cgatcagccc
     1381 ggccttccag agcttgttca ggtaggccgt tgcgtcgcgg aaacgggggt cggccggcgc
     1441 aaacctcact ttcccgtctt cgacgaaaag gttcgagatg ggcagccaga gcgaaccggt
     1501 gtcctgggcc ggcaggccga acgcgtagta cacgaaccag gggccggtgc ggggcacgcc
     1561 gttggggaga tcgccccagg ccatcgcgat ggggatctcg tccgccttgc cgttgccgtt
     1621 caggtcggcg ttcttcatcg ccaccatcag gtcatagaac tcgtcggtgg tagccggcat
     1681 cttccggccg agcttgtcca cccacggctt gtagatgttg tggacgctga gctggcgcat
     1741 ccagctcccc tcgttcaggc gggcgaaggt gtagatgttg ccatccggcg ccgtggtgaa
     1801 cttcatgatg tcgggcttct gcttgatcac ggcctggtag ttcggcatgt acttctggat
     1861 caggccctgc aaggggacga acaccttggc ggccccgtag gtcacgatgt cgaagtccgg
     1921 caggttctcg gagaacgcgt ccggaaggtc gttgctcgcc agcatgaggt tcttcttctc
     1981 ttccatggcg gcggcgggaa cttcgatcca ttctatgtgg acgttggtga gcttctccgt
     2041 ctccttgatg acgggaagct catcaaaggg cttgcgatga agcgggtctt tctgtccgaa
     2101 cacccgcagc gtcaccgtct tgtcaacgat gggatacccg gtggcgtgga aacccgccgc
     2161 ctgctcctcg ccggtgacct gagcgaatgc cggcaccacg aggacggtga ggaccaggaa
     2221 cacgaaccaa tgtctcatgc tgctcctcct cttctcgatt ttctcacacc cgccggctgc
     2281 cgtcaacttt tttcgcggct cctggggccc ggcgcgccgc cggtgagcgg gatttccagc
     2341 accgtcgcgg agcgagccgc gaacgtgtgc acgaaatcgc cgccaaccct tgcggccgga
     2401 cgcggtttca cggcaaccgc ctcgggctcc cggacagtat tgcgcgcatc gggccccgcg
     2461 cccgtcagct cgaagaccgc cgcggagtcc ttcacggaga agccatccag ccggatgcgg
     2521 cactccacgg gttcgtccct atgacggttc accaccgcga ggcacagcag gcgcccggag
     2581 tcctccctca gcgtcgccga ggcatccacg taggggaccc cggacagggg ccccaggccc
     2641 cggggccctc cgttgtcgtg cgtgtcgaac gtctccgaca cggtgaaggt gtcaaagacg
     2701 gttgtcccgg tgtggttggc gtacaagtca aaggcgtggt acagcggcga caggacaacg
     2761 cccgcctcat ccgcgtgaat catccccagg gcgttgacca tctgcgccag gttcgccatc
     2821 cgcacgatgc cgcacatccg ctgcagcgcg ttgaaggtgc ctgccacgaa cagggcgtcc
     2881 ttcagctcgt aaacctcctc aaagaggtcc tgcgggtcct tgcgcgtgcg gtaccagacg
     2941 ttccactcgt cgaaggcaat cgggatacgc cgcgtgagcc cggtggtgaa cgctgccagc
     3001 tcaatggcgg actgcagcat gcggatgcgc ttttccacgt gcaccggccc cgccaccgtg
     3061 gagtagtggt cgtcggtccc gtagtagccg tgctgggcga tgtagtccag gtactggccg
     3121 gccttctcga gcacggtgag gttccagtcc acctccccgc ggttggagcc tacccccacc
     3181 agcgtgatcc cggcggaggc gcgcctcatg agcttggagt actcgcgcac cgcgcgcgag
     3241 tactcttccg ctgagagaca gccgatttcg ttgacgtgct ccgtctcatc gatctcgttg
     3301 ccgactcccc agtagcggac gccgaacggc tcttcgtggc cgttggcgcg gcgcatgcgc
     3361 gcatacgccg tggcgccggt gccgttgcag tactccaccc aggcggctgc ctcctccggg
     3421 gtgccgcttc cgacgttggc gcagatgtag ggctgggcgc cgaggcgctg gcagtactgc
     3481 acgaactcgt cggtgccgaa ccggttcgtt tcgtccttgt tgcgccacgc cgggttgatc
     3541 cgcgtggggc gctcctccac cggacccacg ccgtcacgcc agtgatagcc gtcagcaaaa
     3601 cagccgcccg gccaccgaag gatggagggc cggatgcggc gcatcgcggc aatcacctcg
     3661 tctcggtacc tcccgtgttc gtccagcaca cccccataga tgcatctgcc gaggtgctcg
     3721 atgaagtgcc cgaagatgag cctgtctacc tctcccagga cgcggtccgc gtccactttg
     3781 atggttgccc tcatcgttgc ctccttgccg tccgattgcc gtgccattct ggcgcatgaa
     3841 tcctgtcctg ccaaccgtcg agcccgagtg gacgaggcgg gtccggactc ccgatcttag
     3901 gacggaaagg cgggatgacc cccattgaag ctaccgtacg gcatggcaac cctcggcgcc
     3961 gccgcgtgcg cggccctcgt ggcctgcagt ctctcaacgc agccgccctc ggtccgggac
     4021 tggcttgtct tcggctcgga cgtagcgcac tcaagcgcgc cggccaccgc gggcctcact
     4081 cggagctctg tcgcttcact gcacctgcag caggtgatgc ttgacggaac cgtggatggc
     4141 tccgcggtct acgttcacgg tgcgtcggtg ggaggttccc agcacaacgc gttcttcgtc
     4201 accacgacgt acgggaagac gatcgccatc gacgcggatg acggagccgt gctctgggag
     4261 tacacgccgg caagctat
//
LOCUS       DASRZS010000063         4379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_423582, whole genome shotgun sequence.
ACCESSION   DASRZS010000063 DASRZS010000000
VERSION     DASRZS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423582"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFH83_01525"
     CDS             <1..576
                     /locus_tag="VFH83_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485066.1"
                     /translation="PPRMVPNVQLIEKAVQLIKEDFPSYRIEIKADSVALRDYASMKQ
                     ERVEAIQALTSIFQTGMPMVQALGPKCAQFLLEVGKWLLASTKGSQQIEGVFDRFATE
                     LEAMANQPPPPPPPDPKMQAAQVKAQAEMGKAQANQQQTQLDTQAHVVKTGMDMQVAR
                     TEHQMAMEKLAAETQASIVKQANTAPGVPAV"
     gene            584..763
                     /locus_tag="VFH83_01530"
     CDS             584..763
                     /locus_tag="VFH83_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485067.1"
                     /translation="MTTKQMVDHIEAARQRDIDPDTVKTWIDRGYLKAKAGTDPGRYS
                     IDPDELDDVIAARCG"
     gene            774..977
                     /locus_tag="VFH83_01535"
     CDS             774..977
                     /locus_tag="VFH83_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485068.1"
                     /translation="MYEFQKIHGTPPMNTPGKPTTNKWKAASLAKALKKKTPLSQIED
                     GDDMEMNAMKGGRYSKAGGGIGS"
     gene            979..1194
                     /locus_tag="VFH83_01540"
     CDS             979..1194
                     /locus_tag="VFH83_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007397246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485069.1"
                     /translation="MAKLKAATRNALPARDFAGPDRSYPIEDPGHARAALSRASQNAS
                     PEEQARIRAKVERKFPSIHMAKAIRGK"
     gene            1203..1400
                     /locus_tag="VFH83_01545"
     CDS             1203..1400
                     /locus_tag="VFH83_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485070.1"
                     /translation="MSGKQHRFMEMVAHDPKAAKRVGVPQSVGKDFAEADKGKKFSSK
                     HMANAVRKPQKESATGSGVAM"
     gene            1397..1714
                     /locus_tag="VFH83_01550"
     CDS             1397..1714
                     /locus_tag="VFH83_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485071.1"
                     /translation="MKWIWDEVQQKLVEYDSYRPPERALDAPIMMDRFYENTVAPDGS
                     DIGSRRKHREYMRANGLTTADDYKQTWQRAAEKREAARVGHVPDPRRREAIERAMYRM
                     NKP"
     gene            1730..2875
                     /locus_tag="VFH83_01555"
     CDS             1730..2875
                     /locus_tag="VFH83_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485072.1"
                     /translation="MADLHEALESKYDEIESGIPAEQPAPVEPVAEPEQLEIEPPKEE
                     RPRDESGRFIETKEEKPKAEKPVKAPPAPKPSVTPQPAAGVTTQTPAPEKPVVSQTKP
                     PQSWKPTEREAWNALPPVAQQAVLRREREIQIALQQAAEQSKVGGSFAEAVRPYEVMF
                     RQAGVPAEQYVGDMLRTAHAITQGPPHVQADALAGLIVSSAAHLLQPDRTDANGNPSC
                     PLDRALVARMGNRGAMAPMAAPQPQADPRALVREELAAIQNQAYEQRAQAQAGELAEK
                     LEFFGDVHETMADIIAMWDKQGKTFVTEDDLQRAYTLACSMDPEVSKIMEQRKAAASV
                     ETRQAATQRAKVAASSVRTQPSAAPAAQTAAGRRAALEAAYDDVESR"
     gene            2987..3988
                     /locus_tag="VFH83_01560"
     CDS             2987..3988
                     /locus_tag="VFH83_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HET6485073.1"
                     /translation="MAFANSNVSDLIAAGIESRTGEIADNVLGNNALLAQLRKKGRVK
                     TVSGGTSILQELSFNTNANGGWYSGADLLPVGAQDVISAASFQFKQYAVPVVVTGLEL
                     LQNSGKERTIDLVEARLEVAESTMMNDINTGCYSDGTGSSGKQITGLNAVVPTTAKAS
                     QSDTYGGISRSTWAFWRTSTTTGQDISSATKCQTVMNTQWATQVRGSDRPDLIIMDQL
                     YWADYMASLQAIQRFSDPASADLGFPVVKFITADVVLDPTSGLGVNATGATTKTAYFL
                     NTKYLHFRPHKDRYFVPLSPNKRYALNQDVECQILAFAGNLTCSGAQFQGRSIATTT"
     gene            4001..>4379
                     /locus_tag="VFH83_01565"
     CDS             4001..>4379
                     /locus_tag="VFH83_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485074.1"
                     /translation="MASAYQFIEPGAGFPGISNTGTFNTSSVPAPLAFGLVAGARDPT
                     YGYAEFMFVAGSTNAAGDACLLQAGTAQQLASAGTASQGPVGIAVTAMTATNVYGWVQ
                     VYGVCDYANIGTQGTAAVGLPLYV"
BASE COUNT          902 a         1457 c         1412 g          608 t
ORIGIN      
        1 cctccgagga tggtcccgaa cgtccagctg atcgaaaagg cggtgcagct catcaaggaa
       61 gacttcccgt cctaccgcat cgagatcaag gccgactccg tggccctccg ggactacgcg
      121 tcgatgaagc aggagcgggt cgaggccatc caggcactga cctcgatctt ccagaccggc
      181 atgccgatgg tgcaggcgct gggcccgaag tgcgctcagt tcttgctcga ggtcggcaag
      241 tggctcctcg cgtccacgaa ggggtcccag cagatcgaag gcgtcttcga ccgcttcgcc
      301 acggaactgg aggcgatggc gaaccagcct cctccgccgc ctcctcccga tccgaagatg
      361 caggccgccc aggtcaaggc ccaggccgag atgggcaagg ctcaggccaa ccagcagcag
      421 acgcagctgg atacccaggc ccatgtggtc aagacgggca tggacatgca ggtcgccagg
      481 accgagcacc agatggcgat ggagaagctg gcggcagaga cccaagcgtc aatcgtcaag
      541 caggccaaca ccgcgccggg agtcccggcc gtctgaggta cgcatgacaa cgaagcagat
      601 ggtcgaccac atcgaagcgg caaggcagag ggacatcgac cccgatacgg tcaagacctg
      661 gatcgacagg gggtacctca aggccaaggc tggaacggat ccggggcgat actccatcga
      721 ccccgatgaa ctggacgacg taatcgccgc ccgctgcggc tgagaggtca cgcatgtacg
      781 agttccagaa gatccacggc acgcctccga tgaacacgcc gggcaagccc accacgaata
      841 agtggaaggc ggcgagtctc gcaaaggcgc tcaagaagaa gacgccgctc tcccagatcg
      901 aggacgggga cgacatggag atgaacgcca tgaagggcgg gcgctactcc aaggccgggg
      961 gcggcatcgg gagctaggat ggcaaagctc aaggcagcca cgcgcaacgc cctcccggcc
     1021 agggactttg ccgggccgga tcggtcctac cccatcgagg acccgggcca tgctcgggca
     1081 gccctttccc gcgcgtcgca gaacgcctcc ccggaggagc aggccaggat ccgggccaag
     1141 gtcgagcgca agttcccctc catccacatg gcgaaggcca tccggggcaa gtgatgccca
     1201 gcgtgagcgg caagcagcac cgattcatgg agatggtcgc ccacgacccg aaggcggcca
     1261 aacgggttgg tgtgccacag tcggtgggca aggacttcgc cgaagccgat aagggcaaga
     1321 agttctcctc caagcacatg gcgaacgcag tccggaagcc gcagaaggaa tcggcgacag
     1381 gctcaggggt ggcgatgtga aatggatctg ggacgaggtc cagcagaagc tggtcgagta
     1441 cgacagctac cgtcccccgg agcgcgccct cgatgctccg atcatgatgg accgcttcta
     1501 cgagaacacg gtagccccgg acggctcgga catcggcagc cggcgcaagc atcgcgagta
     1561 catgcgcgcg aacgggctca cgaccgccga tgactacaag cagacgtggc agagggcggc
     1621 agagaagcgc gaggcagcga gggtagggca cgttccagac cccagaaggc gcgaggccat
     1681 cgagcgcgcc atgtaccgca tgaacaagcc gtaacccagg agacccagga tggcggatct
     1741 gcacgaagcg ctggagagca agtacgacga gatcgagagc gggattccgg ccgaacagcc
     1801 ggctcccgtc gagcccgtcg cggagcctga gcagctcgag atcgagcctc cgaaggagga
     1861 gcggcccaga gacgaatccg gccggttcat cgagaccaag gaggagaagc cgaaggcgga
     1921 gaagcccgtc aaggcgcctc ctgccccgaa gccttcggtc actccccagc cggctgccgg
     1981 ggtaacgacc cagacgcccg cgcctgaaaa gccggtcgtc tcccagacca agcctcctca
     2041 gtcatggaag ccgacggagc gggaggcgtg gaacgctctg cccccggtcg cccagcaggc
     2101 cgtgctgcgc cgggagcggg agatccagat cgctctccag caggccgcgg agcagagcaa
     2161 ggtcggaggg tcgttcgccg aagccgtccg cccctacgag gtcatgttcc ggcaggctgg
     2221 cgtccctgct gagcagtacg tgggggacat gctcaggacc gcccacgcca tcacccaggg
     2281 ccctccgcac gtccaggccg atgccttggc cgggctaatc gtctcctctg ctgcccatct
     2341 cctccagccc gaccggacgg acgccaacgg caatccctcc tgtcccctcg accgtgcctt
     2401 ggtggcgagg atgggcaacc gcggggccat ggctccgatg gccgctcccc agccccaggc
     2461 ggatcccagg gcgctagtgc gcgaggagct ggcggcaatc cagaaccaag cctacgaaca
     2521 gcgcgctcag gcccaggccg gggaattggc cgaaaagctg gagttcttcg gcgatgtcca
     2581 cgagaccatg gccgacatca tcgccatgtg ggataagcag ggcaaaacct tcgtcactga
     2641 agacgatttg cagcgcgcct atactctcgc ctgctccatg gacccggaag tgtcgaagat
     2701 tatggagcag agaaaggcgg cagcgtccgt cgagacccgg caggcggcca ctcagcgggc
     2761 gaaagtagcg gcatcgagcg tcaggaccca gccgtcagcc gcacccgcgg cccagacggc
     2821 agccggcagg cgggcggcgc tagaagctgc gtacgacgac gtagagtcac ggtgagagcc
     2881 gtttaggccc actcactgac acaagagccc cggcggcggg gcgtggcgaa gggcgtaggg
     2941 ccctccacgc gcgaagtccg cgaccttttg tcaggtggga gggccaatgg ccttcgcaaa
     3001 cagcaacgtc agcgacctga tcgccgcggg catcgagtcc cgtaccggcg agatcgccga
     3061 caacgttctc ggcaacaatg cgctcctggc gcagctccga aagaaggggc gcgtcaagac
     3121 ggtctctgga ggcaccagca tactccagga attgagtttc aataccaatg cgaacggagg
     3181 ctggtattca ggcgccgatc tgctcccggt tggcgcgcag gacgtgatca gcgcggcctc
     3241 gttccagttc aagcagtacg cggtcccggt cgtggtgacc ggcctcgagc tgctccagaa
     3301 ctccggcaag gagcggacca tcgatctcgt ggaggctcgg cttgaggttg ccgagtccac
     3361 catgatgaac gacatcaaca ccggctgcta ctcggacggc acgggctcga gcggcaagca
     3421 gatcaccggc ctcaacgcgg tagtcccgac caccgccaag gcgtctcagt cggacaccta
     3481 cggcgggatc agccggtcca catgggcctt ctggcgcacc agcaccacta ctggccagga
     3541 catctcatcc gcgaccaagt gccagacggt catgaacacg cagtgggcga cgcaggtccg
     3601 cggttccgac cggccggacc tcatcatcat ggaccagctg tattgggccg actacatggc
     3661 ctccctccag gccatccagc gcttcagcga cccggcttcg gctgacctcg gcttcccggt
     3721 cgtgaagttc atcaccgcgg acgtggtgct cgacccgact tcgggcctgg gcgtgaacgc
     3781 gacgggcgcg accacgaaga ccgcctactt cctgaacacc aaatacctcc acttccggcc
     3841 ccacaaggat cgctacttcg tcccgctctc cccgaacaag cgctatgcac tcaatcagga
     3901 cgtggagtgc cagatcctcg ccttcgccgg caacctgacc tgctctggcg ctcagttcca
     3961 gggccgatcc atcgccacca cgacctaagg agacgagatc atggcttctg cgtaccagtt
     4021 catcgagccc ggcgcaggtt tcccgggcat cagcaacacc ggaaccttca acacctccag
     4081 cgttccggct cccctcgcct tcggcctcgt ggccggggcc cgcgacccca cctacggcta
     4141 cgcggagttc atgttcgtgg cggggtccac gaacgcggcg ggtgacgcct gtctgctcca
     4201 ggcgggcacc gctcagcagc tcgcttccgc cgggaccgca tcccagggtc cggtgggcat
     4261 cgctgtcacg gcgatgaccg cgacgaacgt gtacggctgg gtgcaggtgt acggagtctg
     4321 cgactacgcc aacatcggga cgcagggcac cgcagccgtg ggtctgccgc tctacgtcg
//
LOCUS       DASRZS010000064         4300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1576316, whole genome shotgun sequence.
ACCESSION   DASRZS010000064 DASRZS010000000
VERSION     DASRZS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576316"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="VFH83_01570"
     CDS             complement(<1..1547)
                     /locus_tag="VFH83_01570"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET6485075.1"
                     /translation="MKSHAIWAAGVLLTAALLACKTTPPAQPVAPAALSAAPQSAVQP
                     AADGLAPAGAPGHTTLGMALTFGGADLVKSWTLEIQNAAGTVKSWTGDGTTLPSSVSW
                     DGRNDSGATAPEGSYTARLSVDYQKNYESSIARSPSFVLDLTPPTGSVSFDPSQLTPG
                     PQGIQPTATISIAASSTVARMDSWSLDILAPNGKTFRSFAGKWPTTTATWDGTSTNGM
                     PVAPSTTYSAVATVQDEFGLSAELRDTVAVAEIPNAPQQAMVQPRLSGFSPVSATMAR
                     TIDLLVSIGNQGALKSWKLELLQSELGEEKQWSGDASSVPSVITWDGTMDTGGLAPEG
                     IYTAVLDVDYGEAFQPVQVKSSSFILDVSPPTATLSASPSALTPNGSGGIEPESIGVT
                     ASSKVASIQSWSLDVVGPGSTPVATFSGLDPKARVPWDGSLTGGGQVDPTRSYALQAK
                     IQDVYGNVGLVQVELPGGALPQVTGTVAATARLVGFSPMADSLPQAQTITLSLGQDTS
                     VSAWTLS"
     gene            complement(1557..1784)
                     /locus_tag="VFH83_01575"
     CDS             complement(1557..1784)
                     /locus_tag="VFH83_01575"
                     /inference="COORDINATES: protein motif:HMM:NF022229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET6485076.1"
                     /translation="MEKQIAECARRVIATLAALGEVNVLRLAEHLGERSVLTYQALGW
                     LAHEGRLRYSRRGSQVYVSLGPDRAEAQAPS"
     gene            complement(1887..4271)
                     /locus_tag="VFH83_01580"
     CDS             complement(1887..4271)
                     /locus_tag="VFH83_01580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET6485077.1"
                     /translation="MDIGVRELVETVLPSGDLHSARLARRLMQAGMDAHALVRSDRPD
                     GYQSEVGVSLPVRLGQEEATLRGRIDGLLLSESSVTIEEIKSTAGPLADVAEGGNATH
                     RAQAMVYAAMFCSSRGLDGADVTLTYYSLRDHSTRSFTTGYTRRELSERLSTILDSYA
                     ELIRWERERRADRDRSVRALAFPFASRRKGQDDLMAAVEAALRDGGRLLVRAATGIGK
                     TLAVLYPSIRSLADGSTARIFYLTARGTGRGMAEETLGLLCEQGARLRCVTITARDAI
                     CFQGPQCHTEECPFARGYYDRLGLALADARVGGIFNRQRIEELARRHTVCPFELSLDL
                     TLSADCIVCDYNYLFDPRVSLQRFFARPEGNSVFLVDEAHNLVDRVRAMYSASLSKRA
                     LVAGRRHVDRKLHRQAHQDLGRINDWLLSAKRQCGEENVRYRAAEDAPRDLIELVERS
                     RESLEPVLFDGDAPPLSDGRQELLELYFSLGGFLQTAESFGSAHAAAVQCASGDTVVS
                     LLCLDPSRFIRASTDKGRATVFFSATLTPIASFLRAFGAEPSDPGIDVPSPFPPSNLL
                     VLLDDTVSTRLLDRPSTLDRVAQDLAALCAGDGNYLAFFPSYAYLEAVLPRFQGLAPR
                     ASVLAQRRELTAEDREAFLSRFTVHPDGTLVGFAVLGGIFAEGIDLPGERLVGAAVVG
                     VGLPAVTPERELIRGYYARGEGTGAREAYELPGLARVLQAAGRLIRTETDRGVLLLVD
                     DRYAGRFYQESFPPEWRDHRRVRTASEIARSVDEFRGARPTPQRASIDRPASKD"
BASE COUNT          725 a         1442 c         1481 g          652 t
ORIGIN      
        1 aaggagaggg tccatgcgct caccgacgtg tcctgaccca gggagagcgt gatggtctgg
       61 gcctgcggga gactgtccgc catgggcgag aaacctacga gacgcgccgt cgcggccacg
      121 gtgcccgtga cctgcggaag ggcgccgccc ggaagctcga cctggaccag acccacgttt
      181 ccgtagacgt cctggatctt cgcctggagc gcgtacgacc gggtcggatc cacctggcct
      241 ccgccggtga ggctcccgtc ccacgggacg cgggccttgg ggtccagccc gctgaaggtg
      301 gccacggggg tcgagccggg tccgaccacg tccagggacc agctctggat cgaggcgacc
      361 ttggaggacg cggttacccc gatgctctcc ggctcgatcc ccccgctgcc gttcggggtc
      421 agggcggaag ggctggcgga cagcgtcgcc gtgggtgggc tcacgtccag gatgaacgac
      481 gagcttttca cctgcaccgg ctgaaaggcc tcaccgtagt ccacgtcgag caccgccgtg
      541 tagatgccct ccggcgcaag cccaccggtg tccatggtgc cgtcccaggt gatgacgctc
      601 ggcacggagg aagcgtcccc gctccactgt ttctcttcac ccagctcgga ctggaggagc
      661 tccagcttcc acgacttcag ggctccctgg ttcccgatgg ataccaggag atcgatggtg
      721 cgggccatgg tagcgctcac cggggagaag ccggagagcc ttggctgaac catcgcctgc
      781 tgcggcgcgt tggggatctc cgccacggcg accgtgtccc ggagctcggc cgaaagtccg
      841 aactcgtcct gcacggtggc cacggcgctg tacgtcgtgg acggcgccac cggcattcca
      901 ttcgtcgacg tgccgtccca ggtcgccgtg gtggtcggcc acttcccggc aaagctgcgg
      961 aacgtcttcc cgttcggggc caggatgtcc agggaccagc tatccatccg ggcgacggtc
     1021 gagctcgcgg cgatggagat cgtcgccgtc ggctggattc cttgggggcc cggggtgagc
     1081 tgcgaaggat cgaagctcac ggagccggtc ggtggcgtca ggtccagcac gaagctgggg
     1141 ctcctggcga tggaggattc gtagttcttc tggtagtcca ccgacaggcg cgccgtgtac
     1201 gagccctccg gcgccgtggc gcccgaatcg ttcctgccgt cccagctcac cgaggagggc
     1261 agggtcgtgc cgtccccggt ccagctcttt accgtcccgg cagcgttctg gatttccagc
     1321 gtccagctct tgaccagatc ggcgccgccg aaggtcaggg ccatccccag cgtggtgtgt
     1381 cccggagcgc cggcaggcgc cagaccgtcc gccgcgggct ggacggcgct ctgtggcgcc
     1441 gcggacagag ccgcgggggc caccggctga gccggcggcg tggttttaca ggcaagcagg
     1501 gctgccgtga gcaacacccc ggcggcccag attgcgtggg atttcattcg tgcctcctag
     1561 gacggagcct gggcttccgc acgatcgggt ccgagggaca cgtagacctg actgccccgt
     1621 ctggagtagc gaagccgtcc ctcgtgcgcc agccagccca acgcctggta ggtcaggacg
     1681 ctgcgctccc cgaggtgctc ggccaaccga aggacgttca cctctccgag agcggccagc
     1741 gtggctatga cgcggcgagc gcattccgcg atctgctttt ccaatcgccc aaacccccat
     1801 ccatggagag gctactgatc ctgaagtcga gccagctatc gcgtttgcga tgatcttggg
     1861 atcgaatctg ttctacaggc gggtcctcaa tccttcgatg ccggccggtc gatcgaagcg
     1921 cgctgcggcg tcgggcgggc gcctcggaac tcgtccacgc tgcgggcgat ctcggaggcg
     1981 gtccgaacgc gccgatggtc acgccactcc ggcggaaagc tctcctggta gaaacggccg
     2041 gcgtagcggt catccacgag gagcagcacc cctcgatccg tctccgttcg gatcagcctc
     2101 ccagcggcct ggagcacgcg ggccagaccc gggagctcgt acgcctcccg agcgccggtg
     2161 ccctcgcccc gcgcgtagta gccgcggatc agctcgcgct ccggagtcac ggccggcagc
     2221 cctacgccaa ccaccgccgc gccgaccagc cgctccccgg gaaggtcgat gccctccgcg
     2281 aagatcccgc ccaacacggc gaagcccacc agggtgccgt cggggtgaac ggtgaaccgg
     2341 gagagaaacg cctcccggtc ctccgcggtg agctcgcgtc gctgcgcgag gacggacgct
     2401 cgcggcgcca gtccctgaaa gcgcgggagg actgcctcga ggtacgcata ggaggggaag
     2461 aacgcgaggt agttgccatc gcccgcgcag agcgcggcca ggtcctgggc cacccggtcc
     2521 agcgtcgagg gacgatccag gagccgggtg gagaccgtgt cgtccaacag gaccaacagg
     2581 ttggacggcg ggaagggcga agggacgtcg atcccggggt ccgacggctc ggcgccgaag
     2641 gctcggagga agctggcaat gggggtcagc gtagcggaga agaagacggt ggcccgaccc
     2701 ttgtccgtcg atgcgcggat gaaccgcgag ggatcgaggc acaggagaga gacgaccgta
     2761 tcgccggaag cgcactgcac cgcggcggcg tgcgccgagc cgaaggactc cgcggtctgg
     2821 agaaatccgc ccaggctgaa gtagagctcc aggagctcct gccgtccgtc cgaaagcggc
     2881 ggggcatcgc cgtcgaagag gacgggctcc agggattccc gggaccgctc cacgagctcg
     2941 atcaggtccc gcggcgcgtc ttccgccgcc cggtagcgga cgttttcttc cccgcactgc
     3001 cgcttcgccg acaggagcca gtcgttgatc cgtcccagat cctggtgagc ctggcggtgg
     3061 agcttgcgat ccacatggcg gcgcccggcc acgagagccc gcttggagag agacgccgaa
     3121 tacattgccc ttacgcggtc cacgaggttg tgggcctcat cgacaagaaa cacggagttt
     3181 ccctccggcc tcgcgaagaa gcgctgcagg gaaacgcggg gatcgaagag gtagttgtag
     3241 tcgcagacga tgcagtccgc cgagagggtc aggtccagag agagctcgaa cgggcatacg
     3301 gtatgccggc gggccagttc ctcgatccgc tggcggttga agatcccgcc gacccgggcg
     3361 tcggccagag cgaggccaag ccgatcgtag tatccccgag caaaagggca ttcctcggtg
     3421 tggcactgcg ggccctggaa acagatggcg tcacgggccg tgatggtcac gcaccgcagt
     3481 cgagcgccct gctcgcacag gagtcccaga gtctcttccg ccataccccg gccggtgccg
     3541 cgcgcggtca ggtagaagat gcgcgccgtg gagccgtccg ccagggagcg gatggagggg
     3601 tacaggaccg ccagagtctt gccgatcccc gtggccgcgc gaaccagaag cctcccgccg
     3661 tcgcgaagcg ccgcctcgac cgcggccatg agatcatcct gtcccttccg acgggaggcg
     3721 aatgggaacg caagggcgcg gacggatcga tcccgatccg cccgacgctc ccgttcccag
     3781 cggatcagct cggcatagga gtccaggatc gtggaaaggc gctcggaaag ctcgcgccgc
     3841 gtgtatcccg tcgtgaagct ccgggtggaa tggtcccgca ggctgtagta ggtgagagtg
     3901 acgtccgccc cgtccagacc gcggctggag cagaacatgg ccgcgtagac catggcctga
     3961 gcacggtgcg tcgcgttccc cccctcggcg acatccgcca gaggccctgc agtgctcttg
     4021 atctcttcaa tagtaacgct gctctcggag agaaggaggc cgtcgattct gccacggagg
     4081 gtagcctcct cctggccaag tcgaaccggc agggaaacgc ctacctcgct ctggtagccg
     4141 tccggccgat ccgagcgcac caacgcgtgc gcgtccatcc cagcctgcat cagccgccgc
     4201 gccagccgag cggaatgcag gtctcccgaa ggaaggacgg tctccaccag ctcgcggacg
     4261 ccgatgtcca tccgctattc cggaacgagg agcacctgga
//
LOCUS       DASRZS010000065         7574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774 k141_37430,
            whole genome shotgun sequence.
ACCESSION   DASRZS010000065 DASRZS010000000
VERSION     DASRZS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7574
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37430"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..7562)
                     /locus_tag="VFH83_01585"
     CDS             complement(117..7562)
                     /locus_tag="VFH83_01585"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HET6485078.1"
                     /translation="MQFELFAGVVVGCNSLAISASGFSVTGDLRFPDTFPQGLAGTTC
                     TLSAFSMTWGGVVTKIEAGVASATISLGGFTTEVTNLLFHPGGITLESCIVTLPPTMK
                     SARIGVRNASFDATGAFHGAFVVPTLETDIAGFTLALYDPSLDVPGKQVTFSRAELRM
                     PAMVGGSSIALSGVCLGASGLSFTGGEFRIPDFSIAGGLGFRGVFVKFSLSGASYLIE
                     GGGQAMIPGAGTFGAAVSFVNVSSTYPWGLKRAEFSYQVSGLGLPLGATGLYLNGIRG
                     GLAFGPPEEIPQAVRWMFDSGTRIKVGLSMTDVTGGYSVRADADVWVDVTDWDWAFQG
                     NVALLTGYVRGEVIAALTGKGFYGSFRVSLTFVDGRVDVYVYDDHGTQISGSGSVAFK
                     LLRGCLYDAVVDLGWLGRYEVAIPWSTLQLPGMYAEFGRFRGGAEGFKGYVDVPAFGQ
                     TGVFVPRSGGIVFGGVSQYVLATPSGAKAAGLAPRSPARTARVLSVDQSPMDGATDSY
                     RFVVPGPATAGSAAHARSAARASVPVDPSAEPDGSGSRVERLVFALCYAEGDPVVTAL
                     SPSGRAYSSADPSVETIRTEWGMLVVVQSPDPGDWTLEVAGVLSPDSYQAMVFGKSAR
                     VEVQTRTPAYAAELADTGFTVTGAATNAQAATEVKVFLSRERESYVGQLVATTLVDQA
                     GSFSAAVDSSGLPDGEYFIFTSAGTGDLPDTRSFAPGSILVRHPTSGLRAVDGFTACD
                     NGHGGIDIHFENPAGPRAEGFRLVVEDLSHNTTETILLGFVTDTTIPAFDDGDVLRLT
                     VTAYDQNKTEGPPSRSVTVIMGAHASAVNTVSLAEPTSGVAVTIDSTARAVIRVLSAN
                     AVRTGTALDHAVARLSRAPEGMVVGFDESVWDVSGGTADIACTVYAAEALAPGTYTLV
                     INVRNSGNPSNAFDVSLPVSVSYPAIAVGRVSPESWNNADSTDITVEGRYLFAGTRLY
                     LDQMELAVQSADADRLSATVPAGVTAGAHLVTVVGPGGHQATHVVTVIEPEYRVLGLK
                     TLGSTTAGTTTRYYFTIQALNGFQGSASFSVQTVPSGWDALIDQPSISQGSISTVSVT
                     VPANASPGERTVQIASDRGDLLDLSVQVSAARPDPAVAGLSAYSGFVGDELAIYGSGF
                     DQPISVTLGDRLMPQLASSADLIRVQIPAGASTGLIRVTRGQASSNGRILFVKQAGFV
                     LYGPRSAIRMQAGETQGVSIAISGYAREVGLTVDGGAVIAGADRTSVTPNAVINLEVT
                     VPAGTVSGTFVVHVTATGDGLERTLDVPVVVGNALQIVTTALPDAMEDVSYRQTIEAE
                     NGSGDVSFSLSAGRLPDGLTLTARGVLSGNPDRVGTASFTVEALDAEGHRADCRLSLD
                     VLANGWGQAEMNGGRSRANPIPSPAADWIGWQSAPRTDARTILTGRDRVFRIGQDEVD
                     GLEKATGALVYRLAIATVRAAVAGETLYLLDAEGTLHAVEGRYGNEKWTRDGVAELTT
                     DGTMLCLGTDEGIVLVDEASGTVSAILPGALPAGGLYAWNQGTVLHVDGKALYALAAD
                     GWTLRYSNPEECLADISCDTTGMVLMTAGGRLIVLDQAFREIAHRDIGFVADALALTE
                     GWVIAASGRESAVFHRGTLGPVSDSNWGAPVLAAGRDRYFLADASGVRAINGYSGKLL
                     WSWSGSFVDLALAGGKLFGLGATGQVFCFDAPDNVWPPTTSLEVTPPRPNGENGWYTI
                     QPSFSLSAEDPETFAQGTTYRLGTEPAEAYQRPVSVPEGTTQVVYWSEDSQGWRENDH
                     LATFQVDLGNPVAAGVLSGTVGPIGVYLSAVGVELSAEDPVSGLDRVEYRSGGGAWLP
                     YSSPIGITAEGSYDLDYRARDLAGNVSPTQSLHFTIDLSIPQVDSHGTSQAGIVTLYI
                     EATDTGTGIERVEYGIDGGPLRDYTQPIVLTETGAHDVRYRAVDRAGQWSPWQDRAVT
                     VSAFAPGLLAESLQFHDWQPGRELVLDVQVGERLFAPSEGHDEDNRIKALPAYLVGAN
                     FIRLNRQDRRFDGNDFLHFRVTEDATVYILKHDKSGAVLTGWSLVSRDFPVEPVRYFR
                     GGADVYRRFYRAGEVATIPGTRGGSECWPNLLFVQRSVEQEVRITSPEPGKDLAPLSS
                     VPLAGTNLAQTPGAVRKWEVRCEGGSWQAILGDTVALPYTRATLRCQLRLTLSDASGR
                     ALGAQVEEYSIVNGAELTVYNPGPGWDLPCSSSVELSFRATDIAGRVLSRGEVAWRTS
                     RDRQRWEPVTLQNGLLLSCPAQAGTFYLKASWLEAEGYPREKELAFRVVPAIVPVRIE
                     LGAPVGEEYCLGEVLGVRPSGRTYGFSSDHTDRAESFRTLELVADGHHAMPRIRTDSY
                     IAIEAGERFEVAIGTGRARVGLHVGPLEGRSTDSVRVNGRSINLPPSRQEHIVDVTVE
                     ACARDGVLEIGGSDNLPLMDVTIELLPAGAPPVADVVTVEDLIVLERRHGPDLERGEP
                     R"
BASE COUNT         1320 a         2562 c         2456 g         1236 t
ORIGIN      
        1 aagggtaact cgctgcgatg ggtcgttcgc gatctcttga cctccggacg ccagcacccg
       61 agcggtggcg gtggcgatca tgcatgccag gagaatcggc atacttcggc aacgtctcat
      121 cgtggctcac ctcgctccag atcagggccg tgacgccgct ccagcacgat gagatcctcc
      181 actgtgacga cgtcggcaac gggtggcgcc ccggcaggca gaagctcgat cgtcacgtcc
      241 atcaagggca ggttgtcact gccgccgatc tccagaacac cgtcccgcgc acacgcttcc
      301 acggtgacgt ccacgatgtg ctcctggcgc gaagggggga ggttgatcga tctgccgttc
      361 acccggacgc tatcggtgga tcttccttcc agcgggccga cgtgaagacc gaccctggcc
      421 cgtcccgtac cgatggccac ctcgaatctc tcacccgctt ctatagcgat gtagctgtcc
      481 gtccggatcc tgggcatggc gtgatggccg tccgccacca gctccagcgt tcggaagctc
      541 tccgcgcggt ccgtgtggtc cgaggagaag ccatacgtgc gcccggacgg ccgtacgccc
      601 aacacctcgc caaggcagta ttcttcaccg accggggctc ccagctcgat ccgtaccgga
      661 acgatcgccg ggaccacgcg gaaggcgagc tccttctcgc gtgggtagcc ttcagcctcc
      721 agccagctcg ccttgagata gaaggtccca gcttgcgcgg ggcacgagag caacaaaccg
      781 ttctgaagcg tgacaggttc ccagcgttgc cggtcgcggc tggtgcgcca cgccacttct
      841 cctctggata ggacccgtcc ggcgatgtcc gtggctcgga agctcagctc gacgctcgat
      901 gagcagggga ggtcccaccc ggggccaggg ttgtacaccg tcagctcagc gccgttcacg
      961 atggagtact cctccacctg cgcgcccaaa gcccgccccg aggcgtcaga gagcgtcagc
     1021 ctgagctgac atctcagggt ggcgcgggta tagggcagcg caaccgtgtc ccctaggatc
     1081 gcctgccagg atccgccctc acaccgcacc tcccacttcc ggacggctcc cggagtttgc
     1141 gccagattcg ttccggccag cggcacggag gacaggggcg caagatcctt tccaggctca
     1201 ggagaggtga tccgcacttc ctgctccacg ctcctctgaa cgaagagcag gttcggccag
     1261 cattccgatc ccccccgggt ccccgggatc gtagccacct ccccggcacg gtagaacctt
     1321 cgatagacgt ccgcgccgcc gcggaagtac cggaccggct cgaccgggaa gtcgcggctg
     1381 acgagggacc agccggtcag gaccgcgccg ctcttgtcgt gcttgaggat gtagaccgtt
     1441 gcgtcctcgg tgactcggaa atggaggaag tcgttcccgt cgaatcgacg atcctgccgg
     1501 ttgagccgga tgaagttggc ccccaccagg taggcgggca aagccttgat gcgattgtcc
     1561 tcatcgtgcc cttcgctggg tgcgaagagc ctctcgccca cctggacgtc cagtacgagc
     1621 tcacggcccg gctgccagtc gtggaactgc aggctttccg cgaggagccc gggcgcgaac
     1681 gcgctgacgg ttaccgcgcg gtcctgccac gggctccact ggccggctcg atccacggcc
     1741 cggtaacgga catcgtgcgc gcccgtctcc gtcaacacga tgggctgcgt gtagtcgcgg
     1801 agggggcctc cgtcgattcc atactcgacg cgctcgatgc ctgtgcccgt gtccgttgcc
     1861 tcgatgtaca gcgtgacaat gcccgcctgg ctggtcccgt ggctgtcgac ctgggggatc
     1921 gagaggtcga tcgtgaagtg aaggctctgg gttggtgaga cattcccggc gagatcccgc
     1981 gctcggtagt ccaggtcgta agacccctcc gcggttattc cgatcggact gctgtacggc
     2041 agccatgcgc caccgccgga tcggtactcg acacggtcca gtccggaaac cggatcttca
     2101 gccgagagct cgaccccgac cgctgacagg tacaccccga tgggtccgac cgtgccactc
     2161 aggacgccgg cggctactgg gttgcccaga tccacctgga acgtggccag atggtcgttc
     2221 tcccgccagc cctgggagtc ttcgctccag tagacgacct gggtcgtgcc ctccgggacg
     2281 gacaccggcc tctggtaggc ctccgcaggc tccgtgccga gccggtacgt tgtcccttgc
     2341 gcaaaggtct cgggatcctc ggcgctcaga gagaacgacg gctgtatggt gtaccacccg
     2401 ttctcgccgt tggggcgagg aggcgtgacc tccagcgacg tcgtgggcgg ccagacgttg
     2461 tccggtgcat cgaagcagaa gacctggcca gttgcgccta gcccgaagag cttccctccg
     2521 gcgagggcaa gatccacaaa ggagccgctc cagctccaaa ggagcttgcc ggaatagccg
     2581 ttgatcgccc tcacgccgga cgcatctgcg aggaagtacc gatctcttcc ggcggccagc
     2641 accggggctc cccaattgct gtcggatacc ggccccaggg tcccgcggtg gaagaccgcg
     2701 ctttcacggc cggaggcggc gatcacccac ccctccgtaa gggcgagcgc atccgcgacg
     2761 aagccgatgt cgcgatgagc gatctcgcgg aaagcctggt ccaacactat gagacggccg
     2821 ccggccgtca tgagcaccat gcccgtcgta tcacaggaga tgtcggcgag gcattcctcc
     2881 gggttcgagt accgaagggt ccagccatcc gcggcgagcg cgtagagcgc cttcccgtcc
     2941 acgtggagga ccgtcccctg attccatgcg tacaagccgc ccgccggcag ggcgcccggc
     3001 aggatcgcac tcaccgtgcc ggaggcctca tcgaccagca cgatcccctc gtcagtaccg
     3061 aggcacagca tggtcccgtc cgtggtgagc tccgcgacgc cgtctcgggt ccacttctcg
     3121 ttcccgtacc gcccctccac cgcgtggagc gtgccttccg cgtccagcag atacagcgtc
     3181 tctccggcga ccgcggcgcg gaccgtggcg atggcgaggc ggtagaccag cgcgcccgtg
     3241 gccttctcca aaccgtcgac ctcgtcctgg ccgatccgga agacccggtc acggccggtg
     3301 aggatggtcc gcgcatcggt tctgggcgcg ctttgccacc cgatccagtc agcggctggc
     3361 gacgggatgg gattggcgcg gctgcggcct ccgttcatct cagcctggcc ccagccgttg
     3421 gccagcacgt ccaggctgag ccggcagtcc gcccggtgtc cttccgcatc cagagcctca
     3481 accgtgaaag acgcggtacc aactcggtcg gggttcccgc ttaggacccc acgggcggtc
     3541 agagtgagac cgtcgggtag ccttccggcc gaaagggaga acgagacatc gcccgagccg
     3601 ttctccgcct cgatagtctg ccggtaggag acgtcctcca ttgcatccgg tagtgccgtc
     3661 gttacgatct ggagcgcatt cccaaccacc accggcacat cgagggttcg ctcgaggccg
     3721 tcccctgtgg cggtcacatg gaccacgaag gtccccgaga ccgtacctgc cggaaccgtc
     3781 acctccagat tgatcacggc gttcggagtg acagacgtgc ggtctgcgcc ggcgatgacc
     3841 gcgccaccgt cgacggtgag ccccacttcc cgcgcgtagc ctgagatcgc gatgctcacc
     3901 ccctgggtct ctccggcctg catccggatt gcggaccgcg gcccgtagag gacgaaccca
     3961 gcctgcttca cgaaaaggat ccggccgttg gagctcgcct ggccgcgggt cacccggatg
     4021 aggcccgtcg aggcgccggc ggggatctga acccggatga ggtccgcgga ggaggcgagc
     4081 tgaggcatga ggcgatcgcc caaggtgacg gagatcggct ggtcgaaccc gcttccgtag
     4141 atggcgagct catcccccac gaagcccgaa tacgcggaga ggccggcgac ggccgggtcc
     4201 ggccgtgcgg cgctgacctg gaccgaaagg tcgagcaggt ccccgcggtc actggcgatc
     4261 tggacggtcc gttcgccggg gctcgcgttc gccggcacgg tcacgcttac cgtcgatatc
     4321 gatccttgcg aaatgctggg ctgatcgatc agcgcgtccc agccgctggg gacggtctgc
     4381 acggagaacg aggcacttcc ttggaagccg ttcagcgctt gtatcgtgaa gtagtagcgc
     4441 gtggtggtgc cggcggtggt ggagcccagg gtctttaacc cgagcactcg atactccggc
     4501 tctatgaccg tcacgacatg cgtggcctgg tggcctcctg ggccgactac cgtcaccagg
     4561 tgcgcgcccg ccgtcacccc ggccgggacg gtcgccgaga gccgatcggc atcggccgac
     4621 tgaacggcga gctccatctg gtccaggtac aggcgggtcc ccgcgaagag gtatctgcct
     4681 tccaccgtga tatctgtgga atccgcgttg ttccaagact ccggactcac tctccccacg
     4741 gcgatggccg ggtagcttac agagactgga aggctgacat cgaacgcgtt cgacgggttg
     4801 ccagagttgc ggacgttgat caccagggtg tacgtgccgg gtgcgagggc ttccgcggca
     4861 tagaccgtgc aggcgatgtc cgcggtcccg ccgctgacgt cccacaccga ctcatcgaat
     4921 cccaccacca tgccctccgg cgcgcggctg agtcgggcca cggcatgatc gagggcggtc
     4981 ccggtgcgga ctgcgttcgc agacaggacg cggatgacgg cacgagccgt cgagtcgatc
     5041 gtcactgcca cgcccgacgt cggctccgcc agagaaaccg tgtttacggc cgaggcatgc
     5101 gcgcccatga tcaccgtgac cgaccggctt ggtggcccct cggtcttgtt ctggtcgtag
     5161 gcggtcacgg tcagcctgag gacgtctcca tcatcgaacg cggggatcgt cgtgtcggtg
     5221 acgaaaccga gaaggatggt ttccgtcgtg ttgtgggaga ggtcctcgac cacaagccgg
     5281 aaaccctcgg cacgtgggcc ggccgggttt tcgaaatgga tgtcgatgcc gccgtgcccg
     5341 ttgtcacagg cagtgaagcc atccaccgca cgcaacccgg aagtggggtg acgcaccagg
     5401 atagagccgg gcgcgaagga ccgggtatcg ggaagatccc cagtgccggc gctggtgaag
     5461 atgaaatact ccccgtcggg gagaccggag gaatccacgg cggccgagaa cgacccggcc
     5521 tggtccacca gggtggtggc cacgagttgg cctacgtagg actcgcgctc ccgggagaga
     5581 aacactttca cttcggttgc ggcctgggca ttcgtggccg cgccggtcac ggtgaagccg
     5641 gtgtccgcaa gttcggcggc ataggcgggc gtccgggtct ggacctcgac ccgcgcgctc
     5701 ttgccgaaga ccatcgcctg atagctgtcc ggcgagagga cacccgccac ctccagagtc
     5761 cagtcgccgg ggtcagggct ctggactaca accagcattc cccactcggt ccggatggtc
     5821 tccaccgatg gatcggccga ggagtacgct cgacccgaag gtgagagcgc ggttaccaca
     5881 ggatctcctt cggcgtagca gagcgcgaag acaagccgct cgaccctcga gccggaccca
     5941 tcgggttcag cggaggggtc taccgggacg gacgcccttg ccgcggatct ggcgtgtgcc
     6001 gcagaacctg ccgtcgccgg cccagggacg acgaaccggt aggagtccgt tgccccatcc
     6061 atcggagact gatccacgga cagcacccga gcggtgcggg ccggggaacg cggagcaagg
     6121 cccgcggcct tcgctcccga cggcgtcgcc aacacgtact gcgagacgcc accaaagacg
     6181 atccctccgg agcgaggcac aaacacaccg gtctggccga acgcaggcac atccacgtat
     6241 cccttgaatc cctcggctcc gccgcggaag cggccgaact ccgcgtacat acccggaagc
     6301 tgcagggtgc tccaagggat ggcgacctcg tagcggccaa gccatccgag gtccactacg
     6361 gcgtcgtaga ggcagccgcg caggagcttg aacgccacgc tcccggagcc gctgatctgg
     6421 gtcccgtggt catcgtagac gtatacatcc actctgccat ccacgaacgt gagcgatacc
     6481 cggaacgatc cgtagaaccc cttgccggtc agtgccgcga tgacctcgcc cctcacgtac
     6541 ccggtgagga gcgcgacgtt cccctggaat gcccagtccc agtcggtcac atccacccag
     6601 acgtccgcgt cggcgcggac cgagtagccg ccggtgacgt cggtcatgct gagcccgacc
     6661 ttgatccgcg tgccgctgtc gaacatccac cgcactgctt gcgggatttc ctccggagga
     6721 ccgaacgcca gcccccctcg tatcccgttc aggtagagtc cggtggcgcc caggggaagc
     6781 ccgagccccg agacctggta ggagaactcc gcgcgtttca gaccccaggg atacgtcgag
     6841 gagacgttga caaagctgac cgcggcgccg aacgtgccgg cacccgggat cattgcctgg
     6901 ccgccccctt cgatcaggta gctcgcgccg gaaaggctga acttcacgaa aacgcctcgg
     6961 aatccaaggc ctcccgcgat ggagaagtcc ggaatccgga actcgcctcc cgtgaaggat
     7021 agcccggatg cgccgaggca gaccccgctc agggcgatgc tgctccctcc taccatcgcc
     7081 ggcatgcgaa gctctgcgcg gctgaaggtg acttgcttgc cgggtacgtc caggctgggg
     7141 tcgtagaggg ccagggtgaa tccggcgata tcggtctcca gcgtcgggac tacgaacgcg
     7201 ccgtggaaag cgccagtggc atcgaatgag gcgttgcgca cgccgatccg cgcgcttttc
     7261 atggtcggcg gaagcgtgac aatgcaggac tccagggtga ttccgccggg gtggaaaaga
     7321 agattggtca cctcggtcgt gaaccccccc aggctgatcg tagcggaagc cacacccgcc
     7381 tcgatcttcg tgaccactcc gccccaggtc atgctgaagg ccgaaagggt gcaggtggtt
     7441 ccggccaggc cctgagggaa ggtgtcgggg aaccgaaggt ccccggtgac cgagaacccg
     7501 gatgcgctga tggcaaggga attgcagccg accacgacgc cggcgaacaa ctcgaactgc
     7561 atccgcgccg cgag
//
LOCUS       DASRZS010000066         4381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1963964, whole genome shotgun sequence.
ACCESSION   DASRZS010000066 DASRZS010000000
VERSION     DASRZS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1963964"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VFH83_01590"
     CDS             <1..514
                     /locus_tag="VFH83_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET6485079.1"
                     /translation="HRFTVLTRGQTRTVLMGALLRIYRRRSRLLNSALLRVLARPVSG
                     PVTREFLRDREYGPRIRNLIGRLAAGEDPIFYGAPVVVLIHSGVRIPTPKEDCVLAGF
                     VLSLAALEAGIGACFVTLGQSAINASARCKRILGLPAAEQVHAVVVLGYPVGARQPPS
                     RREVREIRYV"
     gene            complement(599..1723)
                     /locus_tag="VFH83_01595"
     CDS             complement(599..1723)
                     /locus_tag="VFH83_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6485080.1"
                     /translation="MILSKRRVVAAAAALFVLFALPVFAGGTKESGGAPGGTGAKIFI
                     PMVSKGFQFQFWQTVMKGAQAAAAKYGVDMTFEGPPSESDIQTQVQELNNAMAKNPQA
                     ICLAALDTNSVMDQLNQALAKHIPIIGFDSGVPNAPKGAIYATAATNSYAAAGLAADR
                     MFAAIKDKIVAASASSPVMIVDYNQDATSQSVTDRGKGFRDEMIKLITTQTSHTAADI
                     KVTGNPAYIAADSPTSGNQIIIECDVPATTKTTDETNLANAIMNRVQSDHILGIFCSN
                     EGSVSGILSATNDGTALPTQYSGLIVIGFDAGKALKDAIRNKYFFGAITQDPYNIGYK
                     ALELAYMAVKGQPVQNVDTGAKFYDSTNMDNPDIAQLLYD"
     gene            complement(1795..3090)
                     /locus_tag="VFH83_01600"
     CDS             complement(1795..3090)
                     /locus_tag="VFH83_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485081.1"
                     /translation="MGDATAEVSRIQRFRERGLKYILAPGALVFLYVFFGIFGRDYFS
                     YPTLVNIIDAAFYIGFISMGVTFVIISGGIDLSVGTVMMAATLIGGIAFKHGLPMVPS
                     LILIILVGLAFGAFNGILVSRLKMPPFIVTLGTMMISMGVGSIVSNVQSATFPIRGQG
                     GGWFKNLFLYLTPTGGAVPTGAFLLAIVAVICQLMLSNTKLGRYTYAIGSNMEAARLS
                     GVDVKRWQMLVYIIAGGTAGIGGISYAAIYTTVLPAQGQGFELYAIAAAVIGGTSLAG
                     GIGTIWGTMIGVFIMSVLATGLPALSLQAHWQTFFTGVIVIGAVLLDMYRNRMASEVR
                     IETPADLYRASAMEKIESLRKEMASHRASGESLKAAAVRKEIATTRSEMRRTFTRMRA
                     EEKARQAKLRAEERAADREFAEVLRRSSSASSEEREPRS"
     gene            complement(3096..>4381)
                     /locus_tag="VFH83_01605"
     CDS             complement(3096..>4381)
                     /locus_tag="VFH83_01605"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6485082.1"
                     /translation="LEMGIGIIHQELNMMDHLTVAQNIFIGREATRGLFLDKREQNRR
                     TAELFRRLKMNIDPTERLSRLTVGKQQMVEIAKAVSHSLRILILDEPTAALTEAEIAE
                     LFGIMKDLATRGVAMIHISHRLDEIHRIADRVTVLRDGERVDTRATSEVTKQQIINMM
                     VGRVIYEQPKSKSSCAADAPVVLRVNRLNAGRLVRDVSFELRQGEVLGFAGLIGAGRT
                     ETARAIFGADEVQSGTIEIRGHRVSIRSPEDAVGHGLGYLSEDRKRFGLAVNLNVKDN
                     LCMATYDAFVRGLFVHASKVQKVSQEYIERLDIKTPSLEQLLRNLSGGNQQKVVVAKW
                     LIRACDILIFDEPTRGIDVGAKSEIYALMNELVRSGKSIIMISSELPEILRMSDRVVV
                     MCEGRITGVLPIERASQEAIMECATAGRQAVLAGS"
BASE COUNT          702 a         1420 c         1429 g          830 t
ORIGIN      
        1 ccaccggttc acggtactca cgcggggcca gacgcgaacg gtgttgatgg gggcgcttct
       61 gcgcatctac cgtcgtcgga gccggctgct caacagcgca ctgctgaggg tcctggcccg
      121 ccccgtatcc ggtccggtca cgcgggagtt tctcagggac cgggagtacg gcccgcggat
      181 ccgcaacctg atcggtcgcc tggccgccgg ggaggatccc atcttctacg gggcgccggt
      241 ggtcgtgctc atccactccg gcgtgcgcat tccgaccccg aaggaggact gcgtgctcgc
      301 cggattcgtg ttgagcctgg ccgcgctgga ggccgggatc ggagcttgct tcgtgacgtt
      361 gggccagagc gccatcaacg ccagcgcgcg gtgcaagcgc atcctgggac taccggccgc
      421 ggagcaggtc cacgccgtgg tcgtgctggg ataccccgtg ggcgcccggc aacccccaag
      481 ccgccgggag gtccgcgaga tccgctacgt ctgatggaac cgtcgggtcc attccgaaga
      541 cgaaccgggc cggcccgttg gggccggccc ggatctggac ctggcagcga agcctcgctc
      601 aatcgtagag gagctgcgcg atgtccgggt tgtccatgtt ggtggagtcg tagaactttg
      661 cgccggtgtc cacgttctgg accggctgac ccttcaccgc catgtaggcg agctccaggg
      721 ccttgtagcc gatgttgtac ggatcctggg tgatcgcccc gaagaagtac ttgttccgga
      781 tggcgtcctt cagggccttg ccggcgtcga acccgatgac gatgagcccg gaatactggg
      841 tcgggagcgc ggtaccgtca ttcgtcgcgg aaaggatccc gctgacgctt ccctcgttcg
      901 agcagaagat cccgaggatg tggtcggact gcacccggtt catgatggcg ttggccaggt
      961 tggtctcatc ggtggtcttg gtcgtggcgg ggacgtcgca ctcgatgatg atctggttgc
     1021 cgctggtggg gctgtccgcc gcgatgtacg ccgggttgcc cgtcaccttg atgtccgccg
     1081 cggtatggct ggtctgggtc gtgatgagct tgatcatctc gtcgcggaat ccctttccgc
     1141 ggtccgtgac ggactggctg gtggcgtcct ggttgtagtc gacgatcatg accgggctcg
     1201 aggcgctggc cgccacgatc ttgtccttga tcgccgcgaa catacggtcc gcagcgaggc
     1261 cggccgccgc gtagctgttg gtggccgccg tggcatagat ggcgcccttg ggggcgttgg
     1321 gaacgccgga gtcgaacccg atgatgggga tgtgcttggc gagcgcctgg ttgagctgat
     1381 ccatgacgga gttcgtgtcg agcgcggcca ggcagatggc ctgcggattc ttggccatgg
     1441 cgttgttgag ctcctgcacc tgcgtctgga tgtcactctc ggacggggga ccctcgaacg
     1501 tcatgtccac gccgtacttc gcggccgcag cctgggcgcc cttcatgacc gtctgccaga
     1561 actggaactg gaagcctttc gagaccatgg ggatgaagat cttggcgccg gtcccgcccg
     1621 gtgcgcctcc gctttccttg gtcccaccgg cgaacaccgg cagcgcaaag agaacgaaca
     1681 acgccgcggc cgccgcgacc acccgtcgtt tggaaagaat catacaaacc tcctggtttg
     1741 attctggatc cgggcttcgc ccgggtcttc ttggcacctc atgtctcccg cggtctagga
     1801 ccgcggctcc ctttcctctg aagaagcgga cgacgagcgc cgcagcacct ccgcgaactc
     1861 gcggtcggcc gcccgctcct ccgcgcggag cttcgcctgc cgcgccttct cctccgcccg
     1921 catccgggtg aacgtcctcc tcatttccga tcgcgtcgtt gcgatctcct tgcggacagc
     1981 cgccgccttg agcgactccc cggaggcgcg gtgggaggcc atctcctttc gcagggactc
     2041 gatcttctcc atggccgagg cgcggtacaa gtccgccggg gtctcgatgc gcacctcgga
     2101 ggccatgcgg tttcggtaca tgtccaggag cacggcaccg atgacgatca cgccggtgaa
     2161 aaaggtctgc cagtgcgcct gcaggctcag ggccggaagg ccggtagcca ggacggacat
     2221 gatgaacact ccgatcatcg tgccccagat cgttccgatg cctcccgcca gcgatgtccc
     2281 gccgatgacc gccgccgcga tggcgtagag ctcgaaccct tgcccttgcg ctggaagcac
     2341 ggtggtgtag atggccgcgt acgagatccc gccgatcccc gcggtaccgc cggcgatgat
     2401 gtagaccagc atctgccacc gcttcacgtc gacgccggac aggcgggccg cttccatgtt
     2461 gcttccgatg gcgtaggtgt agcggccgag cttggtgttg gacagcatga gctgacagat
     2521 cacggcgacg attgccagca ggaacgcccc ggtgggcacc gcgccgccgg tgggcgtaag
     2581 gtacaggaac aggttcttga accatcctcc gccctggccg cggatgggga acgtggcgct
     2641 ctgcacgttg gaaacaatgg acccgacacc catggagatc atcatggtgc cgagggtcac
     2701 gatgaagggc ggcatcttca gccgggagac caggatgccg ttgaacgccc cgaaggcgag
     2761 ccccaccagg atgatcagga tcagggacgg gaccatgggg agcccgtgct tgaaggcgat
     2821 cccgccgatg agcgttgccg ccatcatcac ggtgcctacg gacaggtcga tcccgccgct
     2881 gatgatgacg aacgtgacgc ccatcgatat gaacccgatg tagaacgcgg cgtcgatgat
     2941 gttcaccagc gtgggatagc tgaagtagtc ccgaccgaag atcccgaaga agacgtacag
     3001 gaacaccagg gcgcccggtg ccaggatgta cttgagcccc cgctcccgaa accgctggat
     3061 gcgactgact tcggctgttg cgtctcccat attcctcagc tcccggccag gaccgcctgg
     3121 cgccccgccg tggcgcactc catgatggct tcctggctcg cccgctcgat gggcaacact
     3181 ccggtgatcc gtccctcgca catgacgacg acccggtcgc tcatgcgcag gatctccgga
     3241 agctccgatg agatcatgat gatggacttc cccgaccgca ccagctcgtt catcagggcg
     3301 tagatctcgc tcttggcgcc cacgtcgatt ccccgcgtcg gctcgtcgaa gatcaggatg
     3361 tcgcaggcgc ggatcagcca cttggccacg accaccttct gctggttgcc cccggagagg
     3421 ttcctcaaga gctgctccag ggacggcgtc ttgatgtcga gccgctcgat gtactcctgg
     3481 ctgaccttct gcaccttgct cgcgtggacg aacaggccgc ggacaaaagc atcgtaggta
     3541 gccatgcaca ggttgtcttt cacgttgagg ttgaccgcca ggccgaagcg cttgcggtcc
     3601 tccgacaggt accccagccc gtggcccacc gcgtcctcgg gggaccggat ggacacgcgg
     3661 tgaccgcgga tctcgatggt cccgctctgg acctcgtccg cgccgaaaat ggcacgcgcc
     3721 gtctcggtgc gtccggctcc gatgaggccg gcgaacccga gcacctctcc ttggcgcagc
     3781 tcgaaactca cgtcccgcac cagtcgcccc gcgttcaacc ggttcacgcg caggacgacc
     3841 ggcgcgtcgg cggcgcagga gctcttcgat ttgggttgtt cgtagatcac gcggccgacc
     3901 atcatgttga tgatctgctg cttggtcact tccgaggtcg ctcgggtgtc cacccgttcg
     3961 ccgtcgcgaa gcacggtgac ccggtccgcg atgcggtgga tctcgtcgag ccgatgggag
     4021 atgtggatca tggcgacgcc ccgcgtggcc aggtccttca tgatgccgaa cagctcggcg
     4081 atctccgctt cggtcagcgc cgccgtcggc tcatccagga tgaggatgcg gaggctgtgg
     4141 gagaccgcct tggcgatctc caccatctgc tgctttccca cggtcaggcg ggagagccgc
     4201 tcggtcggat cgatgttcat cttcagccgt cggaacagct cggcggtacg tcgattctgt
     4261 tcacgtttgt ccaggaagag accccgggtc gcttcgcggc cgatgaagat gttctgggcc
     4321 acggtcaggt ggtccatcat gttcaactcc tgatggatga tcccgatccc catctccagg
     4381 g
//
LOCUS       DASRZS010000067         7238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2345881, whole genome shotgun sequence.
ACCESSION   DASRZS010000067 DASRZS010000000
VERSION     DASRZS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2345881"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VFH83_01610"
     CDS             <1..719
                     /locus_tag="VFH83_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6485083.1"
                     /translation="AMRGVNMPYGYTPSYGGDQSNMSSAFQSLALALIASILLVYLIL
                     VILYESFLTPLIRMMSIPCGVIGAFAALALTGKAINIISFIGLIMLDGLISKNGTLLI
                     DYTNTLVKRGMGLREALVEAGITRLRPIVMTSVTMIVGMLPLALSLGASSEIRSGMAV
                     VLIGGLVTSTLLTPLLLPVVYTLLDDLRGGRGRQDAASGARRWSRRRRAAETVAQGVY
                     DSNGAYDPNGAYDPNGGYEQ"
     gene            716..1972
                     /locus_tag="VFH83_01615"
     CDS             716..1972
                     /locus_tag="VFH83_01615"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6485084.1"
                     /translation="MTVGNHTVQRTLTALAAGAAAAAMLLALAGCQRSSTTAETTARS
                     VSVARAARATIEIRLDYPARIRPRQEIVISPKVAGRVAQVLVDVGQRVTAGQLLFTLE
                     SIDYDAQARQAQAALESAKANLTRTSDSSLSSQVIQAQAAAQQAQVQYDDAKDVAART
                     QKLFDAGTATRQQLDTAQARLSSAQIALDAANQSLSLLQQKGGPQSTGLASTQVDQAQ
                     AASDLAQSQLSNTRIVSPITGLIASRAVDPGELVSSAVPAIVVIDVSTLDAETSVEEG
                     VVNTVHVGEDVTVGVDAANVSGVAGVVQSVSPAADPRTLAYTVKVRLSQPPESVRPGM
                     FARVSFPVDRRVNVLTIPNSAIQSESGVQYVYAVVNGVLRRVDIQTGLSDASLTEVLG
                     GLSEGAVVVTEGQSFLNDGQKVTPAS"
     gene            1991..3763
                     /locus_tag="VFH83_01620"
     CDS             1991..3763
                     /locus_tag="VFH83_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485085.1"
                     /translation="MRLLLSYLRPHRGIVILAVFLAVVNQVFSLLDPQIFRLIVDRYA
                     TRIGSIPLAEFIRGAGLLTLASIGVAMVSRVAKNFQDYFLNVASQSAGAALYADGIAH
                     SLRLPYRAFEDQQSGAVLQQLQKARSDSRDMIGQSVNTLFISALSFVLVVGYSFWVHW
                     SIGLSLVVLSPLLGIVVSSLARRIKVVQGRIFAQTNALAGATTESLRNIELIKSLGLE
                     TQEIDRLNATNSQILELELAKVKTVRTLMFYQGTAINFMRSVLIFQLLILAYFKLITL
                     GELFSLLFYSFAIFSPLGELGNVISKYQETRASLENVRRILAQEPAPSNPAGAVPDGV
                     ESIEFSNVTYRHPGAREPALLGASFQARSGESIAFVGPSGAGKSTLVKLLLGLYAPNE
                     GSILWNGIDSRKVNYDLLRKRVGFVPQSIELFAGTIRDNLTFVRPEATDEECLASLQA
                     AQLAGLLERSRDGLDTRIGEGGLKLSGGERQRLAIARALLRRPDLLIFDEATSSLDTE
                     TEKEITRTINQIIATRPSFVTVLIAHRLSTIAGADRIVVLKKGRVMEQGGHLELLRRK
                     GLYYRLWQQQGLDHEEPEVVAVGA"
     gene            3854..4594
                     /locus_tag="VFH83_01625"
     CDS             3854..4594
                     /locus_tag="VFH83_01625"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HET6485086.1"
                     /translation="MKRFYRVIPACLLILVAAGAFAGGRYEGRFGDWVSGSGYVRTES
                     RSVPDFNAIEVDGMGTVTLSQGLVQSVSVETDDNILPMITTEVIGGVLHLGTLPDTHI
                     THVTKLEYRITAASIEGITIAGSGSVRGVTPLRSERMTLEIRGSGDIGTEVAVRSLEA
                     RIGGSGSISVQGRASELSVGIDGSGSLEARRLASSRATVRIAGSGGASVNAARTLDVN
                     IAGSGSVAYTGAAVLSVQSSGSGSVRRF"
     gene            4688..5938
                     /locus_tag="VFH83_01630"
     CDS             4688..5938
                     /locus_tag="VFH83_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485087.1"
                     /translation="MTSRERLRKAIHHEQPDRPPLDLGSTLVTGIQASAYARLKRALG
                     ITGGAVRVYDPYQMLAEVEDSVKKALGVDTFGIQLPTNFFGYRNENWKPFRMFDGTEV
                     LVPGKFEYDVLPNGDIVQYPKGDRGARPSGRMPQGGFYFDSIVRQEPIDEARLDPKEW
                     VAQTYSLYTEEELRFLEETSRRWFEESDYGLLGNFWGAGFGDIAVVPGPAVLEPKGIR
                     DPEEWYVSSIARKPYVKEIFHLQFELQMKNLALYREAVGDRIDVVVMSGTDFGSQNGP
                     FISPDAYRELFKPLHKAMNDWVHGHTGWKTFYHTCGSIVAYLDDFAEAGVDILNPVQI
                     SAAGMSPEFLKQRYGERFVFWGGAIDVQHTLTFKTAPEVREEARRNTAIFGAGGGFVF
                     NNVHNIQATVPVENILALLQSVTA"
     gene            5966..6403
                     /locus_tag="VFH83_01635"
     CDS             5966..6403
                     /locus_tag="VFH83_01635"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HET6485088.1"
                     /translation="MSNKYAASNNAGRERLEKLISRLSENDYTRSVGTSGWNVAAALG
                     HLAFYDRRASVLIARYRKDGVKPSESDTDVINDALALLCAGVGSAMGRIAREAAIACD
                     RDIDQIPADLLARMETKDSSVRLDRGLHRAHHLDLIEKALGGK"
     gene            complement(6456..7073)
                     /gene="udk"
                     /locus_tag="VFH83_01640"
     CDS             complement(6456..7073)
                     /gene="udk"
                     /locus_tag="VFH83_01640"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0046108 - uridine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET6485089.1"
                     /translation="MKIVGIAGGSGSGKTTVVSRITEIVRDFALVAQDNYYKSAENIT
                     NANIAELKPRSADFDHPDAFDSDLLRDQLEALRAGQAIRMPQYDFVRNRRTEETVLVE
                     PRPLVILEGIMIYTEARIRSLIDLKLYVDTPDDIRFIRRLRRDIRERGRTVDSVIDQY
                     VEKVRPGHYEFVEPTKVFADLIIPEGGHNDRAMEVLIAFLAKIAG"
     gene            complement(7131..>7238)
                     /locus_tag="VFH83_01645"
     CDS             complement(7131..>7238)
                     /locus_tag="VFH83_01645"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004746837.1"
                     /note="this stereospecific enzymes reduces the R isomer of
                     methionine sulfoxide while MsrA reduces the S form;
                     provides protection against oxidative stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HET6485090.1"
                     /translation="GLRYCMDSASLRFVPVADMAKEGYSQYLYLFNAQG"
BASE COUNT         1214 a         2413 c         2399 g         1212 t
ORIGIN      
        1 gcgcgatgcg cggcgtcaat atgccgtacg gctacacccc gagctacgga ggggatcagt
       61 ccaacatgtc cagcgccttc cagtcccttg cgctggcgct gatcgcctcc atcctcctgg
      121 tgtacctgat cctggtgatc ctctacgagt cgttcctcac gccgctcatc cgcatgatgt
      181 ccatcccctg cggcgtgatc ggcgccttcg ccgctcttgc cctgaccggc aaggccatca
      241 acatcatctc gttcatcggc ctgatcatgc tcgatggtct gatctccaag aacggcaccc
      301 tgctcatcga ctatacgaac acgctggtga agcgcggcat ggggctgcgg gaggcgctgg
      361 tggaggccgg catcacccgg cttcggccca tcgtgatgac ctccgtgacc atgatcgtag
      421 gcatgctgcc cctggcgctc tccctgggcg cttccagcga gatccgatcc ggcatggcgg
      481 tggtcctgat cggcggactg gtcacctcca cgcttctcac gccgctgctc ctgcccgtgg
      541 tgtacacgct gctggacgat ctgcgcggcg ggcgcggccg gcaggacgcg gcgtccggcg
      601 cgcgccgctg gagccgccgg aggcgcgccg cggagaccgt tgcccagggg gtatacgact
      661 cgaacggagc ctacgacccc aacggcgcat acgatcccaa cggaggatac gagcaatgac
      721 ggttggcaac cacacggtac agcggacgct cacggcgctt gccgccggcg cggcggcggc
      781 tgcgatgctc ctggcgctgg ccggctgcca gcgttcctcc accaccgcgg agaccacggc
      841 gcggagcgtc tccgttgccc gcgccgcgcg cgccaccatc gagatccgcc tggactatcc
      901 ggcgcgcatc cggccgcggc aggagatcgt gatatcgccc aaggtggccg gccgggtggc
      961 ccaggtgctg gtggacgtgg gccagcgcgt cacggcgggt cagctcctct tcacgctgga
     1021 gtccatcgac tacgatgccc aggcgcgcca ggcccaggcc gccctggaga gcgccaaggc
     1081 caacctgacc cggaccagcg actcgtccct ttcctcccag gtgatccagg cccaggcggc
     1141 cgcccagcag gcccaggtcc agtacgacga cgcgaaagac gttgcggctc ggacgcagaa
     1201 gctcttcgat gccggcacgg ccacccgcca gcagctcgat acggcccagg cgcgcctgag
     1261 ctcggcgcag atcgcgctgg atgccgcgaa ccagagcctg tccctgcttc agcagaaagg
     1321 cggcccccag agcacggggc tggcgtccac ccaggtggac caggcgcagg ccgcatcgga
     1381 cctggcgcag tcccagctct ccaacacccg catcgtctcc ccgatcaccg gactgatcgc
     1441 gtcccgcgcc gtggacccgg gggagctcgt gtccagcgcg gtcccggcga tcgtggtgat
     1501 cgacgtgagc accctggacg cggagacgag cgtggaagag ggtgtcgtga acacggtcca
     1561 cgtaggggag gacgtcacgg tgggcgtgga cgcggcgaac gtgtccgggg tggcgggggt
     1621 ggtgcaatcc gtgagccccg ccgcggaccc gcgcacgttg gcctacacgg tgaaggtccg
     1681 gctgagccag ccgccggagt ccgtccggcc gggcatgttc gcccgggtga gctttcccgt
     1741 ggaccgccgc gtcaacgtgc tcaccatccc gaactctgcc atccagagcg aaagcggcgt
     1801 gcagtacgtc tacgcggtgg tgaacggggt cctgcggcgc gtggacatcc agaccgggct
     1861 ctccgatgcg tccctcacgg aggtcctcgg gggcctcagc gaaggggccg tcgtggtgac
     1921 cgaagggcaa agcttcctca acgacggaca gaaggtgacg ccggcgtcct gagggagcta
     1981 tcttccctcc atgcgccttc tgctctcata cctccgaccc caccggggga tcgtcatcct
     2041 cgcggtgttc ctggcggttg tgaaccaggt gttctccctg ctggacccgc agatcttccg
     2101 cctgatcgtt gaccgatacg ccacgcgcat cggctcgatc ccgttggcgg agttcatccg
     2161 cggcgcgggg ctgctcaccc tggcctccat cggcgtagcc atggtgagcc gggtggcaaa
     2221 gaacttccag gactacttcc tgaacgtggc gtcccagtcc gcgggcgccg cgctgtacgc
     2281 ggacggcatc gcccattcgc tccgcctccc ctatcgggcg ttcgaggacc agcagagcgg
     2341 cgcggtcctg cagcagctac agaaggcgcg tagcgacagc cgggacatga tcggccagtc
     2401 cgtgaacacc ctcttcatct cagcgttgag cttcgtgctg gtggtcggct acagcttctg
     2461 ggtccactgg tccatcggcc tgtccctggt ggtgctgtct ccgctcctgg ggatcgtggt
     2521 gtcctccctg gcccgtcgga tcaaggtggt acagggcagg atcttcgcgc agacgaacgc
     2581 gctggccgga gctaccacgg agagcttgcg caacatcgag ctcatcaaga gcctgggtct
     2641 ggagacccag gagatcgatc ggctgaacgc caccaactcc cagatcctcg agctggagct
     2701 ggccaaggtg aagacggtga ggaccctgat gttctaccag ggaacggcca tcaacttcat
     2761 gcgctcggtg ctcatattcc agctcctgat cctggcctac ttcaagctga tcaccctggg
     2821 agagcttttc agcctcctct tctactcctt tgccatcttc tctccgctgg gagagctggg
     2881 caacgtcatc tccaagtacc aggagacccg cgccagtctg gagaacgtgc gccggatcct
     2941 ggcccaggag ccggcgccct ccaacccggc cggcgcggtg ccggacggcg tggagtccat
     3001 cgagttctcc aacgtgacct atcggcatcc gggcgcacgg gagccggcgc tcctgggcgc
     3061 ttcgttccag gcccgatcgg gggagagcat cgcgttcgtg ggaccttccg gcgctgggaa
     3121 gagcacgctg gtcaagctcc tcctgggtct gtacgcgccg aacgagggca gcatcctctg
     3181 gaacggcatc gattcgcgca aggtgaacta cgacctgctg aggaagaggg tcgggttcgt
     3241 gccccagtcc atcgagctct tcgccgggac cattcgggac aacctcacgt tcgtgcgacc
     3301 ggaggccacg gacgaggagt gccttgccag tctccaggcg gctcagctcg ccggcctgct
     3361 ggagcggagc cgggacgggc tggacacgcg gatcggcgag ggtggcctga agctctccgg
     3421 aggcgagcgc cagcggctgg ccatcgccag ggccttgctg cgccggcccg accttctcat
     3481 cttcgacgaa gccaccagct ccctggacac cgagacggag aaagagatca cccggacgat
     3541 caaccagatc atcgcaacac gacccagctt cgtgaccgtc ctgatcgccc atcggctctc
     3601 gaccatcgcg ggcgcggacc gcatcgtggt gctcaagaag ggccgggtca tggagcaggg
     3661 tgggcacctg gagcttctcc gccgcaaagg gctgtactac cgactgtggc agcaacaggg
     3721 gctggatcac gaggagcccg aggtcgtggc ggtcggggcc tgacgggagc gccgcgggag
     3781 gctcccgggc gcgctcttgc ggtgacactt tcccccaggc tcgtggttag agtggggaaa
     3841 ggaaaaacgg atcatgaagc gattctaccg tgtcattccc gcatgcctgc tcatcctcgt
     3901 cgcggcgggc gccttcgccg gcggccgcta cgaaggtcgg ttcggagact gggtcagcgg
     3961 gtccgggtac gttcggaccg agagccggtc cgtgccggac ttcaacgcca tcgaggtgga
     4021 tgggatgggg acggttacgc tgagccaggg gctggtccag tccgtctccg tcgagacgga
     4081 cgacaacatc ctgcccatga tcaccacgga ggtgatcggc ggggtgctcc acctggggac
     4141 gctgccggac actcatatca cccacgtcac gaagctggag taccggatca ccgcggcctc
     4201 catcgaaggg atcaccatcg cggggtccgg gagcgtgcgc ggggtcacgc cgcttcgttc
     4261 ggagcggatg accctggaga tccggggatc cggcgacatc ggaaccgaag tggccgtgag
     4321 gagcctcgag gcccggatcg gtggatccgg cagcatcagc gtgcaaggcc gcgcctcaga
     4381 gctttcggtg ggcatcgacg ggtccggctc gctggaggcg cgcaggctcg cgagctcccg
     4441 ggcaacggtc cggatcgccg gctccggcgg agcatccgtg aacgccgccc ggaccctgga
     4501 cgtgaacatc gccggatcgg gaagcgtcgc ctacacgggc gccgccgtcc tctcggtcca
     4561 gtcgagcggt tcgggctcgg ttcgacgatt ctagcgatcg acggaatacg gacgccggtc
     4621 ccgcttccgc gggaccgcgc cggctccatc tattcccctg acccattcga gttgtagtat
     4681 ggtccgcatg acgtctcgcg agcgactgag gaaagcgatc caccacgagc agccggaccg
     4741 accgccgttg gatctcggct cgacgctggt caccggcatc caggcgagcg cgtacgcccg
     4801 gctgaagcgc gcgctgggca tcaccggcgg cgcggtccgc gtctatgacc cgtaccagat
     4861 gctcgcggag gtcgaggact cggtgaagaa ggctctggga gtggacacct tcggcatcca
     4921 gcttcccacg aacttcttcg ggtatcgcaa cgagaactgg aagccgtttc gcatgttcga
     4981 cggcaccgag gtgctggtcc ccgggaagtt cgagtacgac gtgctgccca acggggacat
     5041 cgtccagtac cccaaagggg accgcggcgc gcggccgtcc ggccggatgc cccagggcgg
     5101 gttctacttc gacagcatcg tccgccagga gcccatcgac gaggcgcggc tcgatcccaa
     5161 ggagtgggta gcgcagacct actcgctgta caccgaggag gagcttcggt tcctggagga
     5221 gacctcgcgc cggtggttcg aggagtcaga ctacggcctc ctgggcaact tctggggagc
     5281 ggggttcggg gatatcgccg tggtccccgg gcccgcggtc ctggagccca aggggatccg
     5341 ggatcccgag gagtggtatg tctcctcgat cgcccgcaag ccttacgtga aagagatctt
     5401 tcacctgcag ttcgagctcc agatgaagaa tctggcgctg tatcgggagg ccgtgggaga
     5461 ccgcatcgat gtagtagtca tgagcggcac cgacttcggc tcacagaacg gaccgttcat
     5521 ctctccggac gcgtaccgcg agctcttcaa gccgctccac aaggccatga acgattgggt
     5581 ccacggccat accggctgga agaccttcta ccacacctgc ggctccatcg tggcctatct
     5641 ggacgatttc gccgaagcgg gcgtggacat cctcaatccc gtgcagatct ccgcggccgg
     5701 catgtcaccg gagttcctga agcagcgcta cggcgagcgg ttcgtgttct ggggaggcgc
     5761 catcgacgtg cagcacaccc tgaccttcaa gaccgccccg gaggtgcgcg aggaagcgcg
     5821 gaggaacacc gcgatcttcg gcgcgggcgg cggtttcgtg ttcaacaacg tgcacaacat
     5881 ccaggccacg gttcccgtgg aaaacatcct cgccttgctt cagagcgtga ccgcctgacg
     5941 cgcgggcgat atcggaggac tctcgatgag caacaagtat gccgccagca acaacgccgg
     6001 gcgcgagcgg ctggagaagc tgatctcccg tctttcggag aacgactata cgcggagcgt
     6061 cggcacctcg gggtggaacg tcgccgcggc cctcggccac ctggccttct acgaccgcag
     6121 agccagcgtc ctgatcgcgc gctatcgcaa ggacggggtg aagccctcgg agtcggatac
     6181 ggacgtcatc aacgacgcgc tggctctgct ctgcgccggc gttggctccg caatgggaag
     6241 gatcgcccgg gaggcggcca tcgcctgtga ccgcgacatc gaccagatcc cggcggacct
     6301 cctggctcgc atggagacca aggactcgtc cgtccgcctg gaccgtgggc tgcatcgggc
     6361 gcaccacctg gacctgatcg agaaggcgct gggcgggaag tagtcgaaga agcggggcac
     6421 ggtcaggccc gccgcacggt cagcccgggc gaccctcagc ccgcgatctt ggccagaaag
     6481 gcgatcagca cctccatggc ccgatcgttg tgcccgcctt ccgggatgat caggtccgcg
     6541 aacaccttgg tgggctccac gaactcgtag tgtccgggcc gcaccttctc gacgtactgg
     6601 tcgatcacgg agtccaccgt ccggccgcgc tcgcggatgt cgcgccggag cctgcggatg
     6661 aagcggatgt catccggcgt atccacgtag agcttcaggt cgatgaggga ccggatgcgc
     6721 gcctcggtgt agatcatgat cccttccaag atcacgagcg gccgcggctc caccaggacc
     6781 gtctcctccg tccggcggtt gcgcacgaaa tcgtactgcg gcatgcggat ggcttgcccc
     6841 gcccgcaggg cctccaactg gtcccgaagc aggtcgctgt cgaaggcatc cgggtggtcg
     6901 aagtcggccg acctaggctt gagctctgcg atgttggcgt tggtgatatt ctcggcggac
     6961 ttgtagtagt tgtcctgggc caccagcgcg aaatcccgga cgatctccgt gattcgcgac
     7021 accacggtgg ttttcccgga gcctgacccg cccgcgattc cgacgatctt cacgcggggt
     7081 agtatagcgg gcgggcgccc gctataccaa tcccgctccg gtctctccgg ttacccctgg
     7141 gcgttgaaca ggtacaggta ctgcgagtag ccctccttgg ccatatccgc cacgggaacg
     7201 aaccgtagcg atgcggagtc catgcagtag cgcagacc
//
LOCUS       DASRZS010000068         3962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_425819, whole genome shotgun sequence.
ACCESSION   DASRZS010000068 DASRZS010000000
VERSION     DASRZS010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425819"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VFH83_01650"
     CDS             <1..732
                     /locus_tag="VFH83_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6485091.1"
                     /translation="DLNAAKDRFLSIIAHDLRNPLHGISSLTELLLDMGEGLTAEKRL
                     GYLRSINTTALQMAKTLSDLMSWARVQRGAFEWKPEPFELCFIVKEASVLLESAAQTK
                     GVVIRNEVPSDHRVFADPIIVLTIIRNLLSNAVKYTRQGGQVTVSAARIGEAVRLSVS
                     DTGIGMSPAQLAKLFRLDSHFSRPGTDGERGTGFGLLFCQELLTKNGLGLEATSQEGA
                     GSTFAFTLPTAREAGGDSRSCSDVP"
     gene            complement(777..1802)
                     /gene="yjfF"
                     /locus_tag="VFH83_01655"
     CDS             complement(777..1802)
                     /gene="yjfF"
                     /locus_tag="VFH83_01655"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HET6485092.1"
                     /translation="MSRRYLAGVRNLPVLVTLGLFIVMFGVGSIAFPGFFAVQNFLNL
                     FIDNSYLLILAVGMTFVIISGGIDLSVGSVLALAGMVTASLLEKVHMSPVLVVPIVLI
                     MGTALGLFMGYLIQAFDLPPFIVTLAGLYLARGLCYIVSLFTIAVKNPFFVSAAHAQV
                     YLFRDDFLSPGAIIAIVVVLAAFFVSRYTKFGRTVFAIGGSEQSAILMGLPVARTKIA
                     IYALSGFCSALGGIVFTLYTLSGYGLNGSGMELDTIAAVVIGGTLLTGGAGNIFGTVL
                     GVFVYGTIQTLVVFQGTLNSWWTRIVIGFLVFVFCVLQRAFEARRNAAPGASEAGRSS
                     RASAGAS"
     gene            complement(1799..2836)
                     /locus_tag="VFH83_01660"
     CDS             complement(1799..2836)
                     /locus_tag="VFH83_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485093.1"
                     /translation="MSWWRSLSRSNIVWGLVALLALLVFNLFFTPHFFRLEIKDGHLF
                     GHLIDILKNGAPIMLLAIGMTLVIATHGIDISVGSLVAVASGAVAILIGGDLAGNPRY
                     PIVVAMLAALAVCTVAGMWNGMLVSRVRMQPIIATLILFVAGRGIAMLFTNSLQLWLY
                     AKPFALLGQGYLLGFPFDIYIVGFILLVTYLFTRRTAVGMFIEAVGINPTAARFSGIN
                     ARNIIFWVYTFSGLCAGISGLIVCSTVMNADGNNAGRLFELDAILAVVLGGTSLNGGK
                     FYLLGSIVGALIIQTLTVTIYAFGVPSDVAPVVKALVVWAFCLLQSPKFRLMITRAVV
                     RRGSVRRGAPA"
     gene            complement(2838..>3962)
                     /locus_tag="VFH83_01665"
     CDS             complement(2838..>3962)
                     /locus_tag="VFH83_01665"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6485094.1"
                     /translation="VSVDVTRTLSSYSIAIQQLVAIARVLDISSRILILDEPTSSLDE
                     AEVDQLFKVIRSLRDEGLAILFITHFLDQTYQIADRITVLKNGELVGEFRTEALPKLD
                     LIARMLGKELSEFSFAGKPEKSAAAAAGAQEASALNVRNLGLRGSIAPFDLSLAEGEV
                     VGLAGLLGSGRTEMARLIFGIDGATQGEILIRGRRTAVRSPQAAMMAGMGFCSEDRKT
                     EGIFSELTVRENIALALQAKRGVFRYIGIHKQLEMADEMIKTLAIVTPSANNKASQLS
                     GGNQQKVMLARWLATQPSILILDEPTRGIDVGAKLEVMGLILDLSRKGMGILFISSEF
                     EEVVRCSTRVAVLRDKVKIAELKGDDVNEASILRTIAGGN"
BASE COUNT          771 a         1305 c         1272 g          614 t
ORIGIN      
        1 gacctgaacg ccgccaagga tcggttcctt tccattattg cgcacgacct gcgtaacccc
       61 ctccacggca tctcgagcct gacggagctg ctcctggaca tgggggaagg ccttacagcc
      121 gagaagcgcc tgggctacct gcgttccatc aacacgaccg ccctccagat ggcaaagacg
      181 ctgtcggacc tgatgagctg ggcccgcgtc cagcggggcg ccttcgagtg gaagcccgag
      241 cccttcgagc tctgtttcat cgtcaaggag gcctcggtgc tcctggagag cgccgcgcag
      301 acgaagggcg tcgtcatccg gaacgaggtc ccgtcggatc accgcgtctt cgccgacccc
      361 atcatcgtcc tgacgatcat ccgcaacctg ctttccaacg cggtgaagta cacgcgccag
      421 ggcggccagg tgaccgtctc cgccgcgcgc atcggagagg ccgtgcggct gagcgtatcc
      481 gacaccggca tcgggatgag ccccgcgcag ttggccaagc tgttccgact ggattcccat
      541 ttctcgcggc ccggaaccga cggcgagcgc ggaaccggat tcgggctcct cttctgccag
      601 gagctcctga ccaagaacgg gctgggtctg gaggccacga gccaggaagg ggccggcagc
      661 accttcgcgt tcacgcttcc gacggcgcgg gaagcgggcg gcgactccag gagctgcagt
      721 gacgtccctt aggcggtcag cgcgccgagg gaccgaggga tgcccgggcc ggcgacttag
      781 gacgcgccgg ccgaggcccg ggaggagcgc ccggcctcgc ttgcgcccgg cgcggcgttc
      841 ctccgcgcct cgaaggccct ctgcaggacg cagaacacga acaccaggaa gccgatcacg
      901 atacgcgtcc accaggaatt gagcgtgccc tggaacacga cgagggtctg gatcgtcccg
      961 tagacgaaca ctccgagcac cgtcccgaag atgtttccgg ccccgcccgt gaggagcgtc
     1021 cctccgatca cgaccgccgc gatcgtgtcc agctccattc cgctcccgtt gagcccgtac
     1081 ccggagaggg tgtacaacgt gaagacgatc cctcccagcg ccgaacagaa gccgctcaac
     1141 gcgtagatcg cgatcttggt cctggcgacc ggaagcccca tcaggatggc ggattgctcg
     1201 ctgccgccga tcgcgaagac ggtcctgccg aacttcgtat agcgggacac gaagaacgcc
     1261 gccaggacga ccacgatggc gatgatcgca ccgggggaca ggaagtcgtc gcggaagaga
     1321 tacacctgcg cgtgcgccgc ggagacgaag aacgggttct tgaccgcgat cgtgaacagg
     1381 ctgacgatgt agcacaaccc gcgggccagg tacaggccgg ccagggtcac gatgaagggg
     1441 ggcaggtcga aagcctggat gaggtacccc atgaacaggc ccagggcggt ccccatgatc
     1501 aacacgatcg ggaccaccag caccgggctc atatggacct tctccagcag gctcgccgtg
     1561 accattcccg ccagggccag gacggagccc acggagaggt cgatgcctcc ggagatgatc
     1621 acgaacgtca tgcccaccgc aaggatcagg agatacgagt tgtcgatgaa aaggttcagg
     1681 aagttctgga ccgcgaagaa tcctggaaac gcgatggaac cgaccccgaa catgacgatg
     1741 aacagcccca gcgtgacgag cacggggagg ttgcggacgc cggcaaggta tcgtcggctc
     1801 atgcgggtgc cccccggcgg acgcttcccc ggcggaccac cgcacgggtg atcatgagcc
     1861 tgaacttggg ggactggagc aggcagaaag cccacaccac gagcgccttc accacgggcg
     1921 ccacgtcgga gggaacgccg aacgcgtaga tggttaccgt gagcgtctgg atgatgaggg
     1981 cgcccacgat gctgcccagg aggtagaact tcccgccgtt cagggaggtc ccgcccagca
     2041 cgaccgccag gatggcatcc agctcgaaga gccttcccgc gttgttgccg tcggcgttca
     2101 tgaccgtgga gcacacgatc agcccggaga tccccgcgca cagaccgctg aacgtgtaca
     2161 cccagaagat gatgttccgc gcgttgatcc cggagaaccg ggccgccgta ggattgatcc
     2221 ccaccgcctc aatgaacatc ccgaccgcgg tccgccgcgt gaacaggtac gtcacgagca
     2281 ggatgaaccc gacgatatag atgtcgaacg ggaagccgag caaatagccc tgtcccagga
     2341 gggcaaacgg cttggcgtac agccagagct gcagcgagtt ggtgaacagc atggcgatgc
     2401 cgcggcccgc gacgaacagg atcagcgtgg cgatgatggg ctgcatgcgc acccgtgaca
     2461 ccagcatccc gttccacatg cccgccaccg tgcacaccgc caacgccgcc agcatggcca
     2521 ccacgatggg gtaccggggg ttgcccgcca ggtccccacc gatgaggatg gcgaccgctc
     2581 cggacgccac ggccacgagg gaacccaccg agatgtcgat cccgtgggtg gcaatgacga
     2641 gcgtcatgcc gatggccagc agcatgatgg gggcgccgtt cttcaatatg tcgatcaggt
     2701 ggccgaacag gtggccgtcc ttgatctcca ggcggaaaaa gtgcggcgtg aagaacaggt
     2761 tgaagaccag gagggccagg agggcaacca ggccccacac gatgttgctg cggctcaggg
     2821 atctccacca ggacatctca gttccccccc gcgatggtcc gcaggatgct cgcctcgttc
     2881 acgtcatcac ccttcaactc ggcgatcttc accttgtctc ggagaacggc aacccgcgtg
     2941 ctgcaccgca ccacctcctc aaactcggat gagatgaaca ggatgcccat ccccttgcgg
     3001 ctcaggtcga ggatcagccc catgacctcc agcttggcgc cgacgtcgat gccgcgcgtc
     3061 ggctcgtcca ggatcagaat ggacggctgg gtggccagcc accgggccag catcaccttc
     3121 tgctggttgc cgccgctgag ctggcttgcc ttgttgttgg cgctgggagt gacgatggcc
     3181 agcgtcttga tcatctcatc cgccatctcg agctgcttgt ggatcccgat gtaccggaac
     3241 actcccctct tggcctggag ggcaagggcg atgttctccc ggacggtcag ctcggagaag
     3301 atcccctcgg tctttcggtc ctccgagcag aatcccatcc ccgccatcat ggcggcctgg
     3361 ggggagcgga ccgcggtccg ccgaccgcgg atgaggatct ctccctgcgt ggcgccgtcg
     3421 atcccgaaga tcagccgcgc catctcggtc cggcccgagc ccaggagacc ggccagaccc
     3481 accacctctc cttcggccag gctcagatcg aacggcgcga tggacccacg caggccgagg
     3541 ttgcgaacgt tgagcgcgct ggcctcctgg gcgcccgccg cggccgccgc gctcttctct
     3601 ggcttcccgg cgaagctgaa ctcggacagc tcctttccga gcatccgggc gatgaggtcg
     3661 agcttgggaa gcgcctccgt tctgaactct cccaccagct caccgttctt gaggaccgtg
     3721 atccggtccg cgatctggta ggtctggtcg aggaagtgag tgatgaacag gatggccagc
     3781 ccttcatcgc gaagggagcg gatgaccttg aagagctggt ccacctcggc ctcgtcgagg
     3841 ctggaggtgg gttcatccag gatcaggatc ctggaggaga tgtccaggac tcgggcgatg
     3901 gccaccagtt gctggatggc gatggaatac gacgagagcg tccgcgtgac atcgaccgac
     3961 ac
//
LOCUS       DASRZS010000069         5447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3503621, whole genome shotgun sequence.
ACCESSION   DASRZS010000069 DASRZS010000000
VERSION     DASRZS010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3503621"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VFH83_01670"
     CDS             complement(<1..830)
                     /locus_tag="VFH83_01670"
                     /inference="COORDINATES: protein motif:HMM:NF037180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485095.1"
                     /translation="MRLEVVNRPEFPDGRARRLLPSLARDVSLGIRAVSIVDVFLIQG
                     APALTPHLAEEVFCDGVSQRLLVDRFAAEDSPGWDYLVEVTVRPGVTDPVALTAREAL
                     RLCLEDSAESASIQTAVQYLVDGGGQAALDPGELARFFHNPLIQSAKCITRSQWREGT
                     RPPQSYAGSIAPSPSQVRLIELEGLAADSLSALSRERLLALSAEEMLAVQRYYAREDV
                     REDRRAHGLSTAATDVELEMIAQTWSEHCKHKIFNARVRYSEGGREQVIQSLFRTYIR
                     S"
     gene            complement(845..2125)
                     /locus_tag="VFH83_01675"
     CDS             complement(845..2125)
                     /locus_tag="VFH83_01675"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET6485096.1"
                     /translation="MKPDLARLIEGNQLVRGLTASDVRRMAGLFRAERFAVGETISQV
                     GSADRLFLVSEGRVQLSDPITGGVALAQVEAGGFFGERSLFPDQPARRQVARAVTEVR
                     GLSADGVELGALLRRFPRLAGGILANVSLSLARRNDQLAEDLRLDRLDVERKAEERAL
                     EYENSHRRIRRELALAQSIQRNLLPERRKSYPGVTVAAEYLPCDELGGDITGVYQIDE
                     RRLGIYGGDICGHGVYAAMVMSYVKKLIETSFKRMFINRQYVVKPPGAILTSINESFM
                     QEISQSDPEIYFTLFLGVLDTRQLTFEYSSAGIHVPPLVISRGSAQRLFDLSDFPIGH
                     VRGHEYQTSHTTFASGDGFLFVSDGVIEAVRGVESFGMERLMTEAQRIIGEAGVLDLD
                     RLMASVREFLGGEPPLDDMCLLTLSFDPSEDPAR"
     gene            2256..3026
                     /locus_tag="VFH83_01680"
     CDS             2256..3026
                     /locus_tag="VFH83_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET6485097.1"
                     /translation="MADILVFGAHPDDAEFGMGASMVKFVRTGASVALCVLTRGEAGT
                     FGNGEMREQEMREAARRLGGEIEILSFSDCAIFDTYEARVILAEVIRRHRPKLIFAPY
                     HTNPGSHKDGGAHPDHWATGAIVRSATRYARFAGLKEVKGEPWNPEYVLYYMMPRNRV
                     PTLVNDVSEYIEEWESIARSHQSQLAIRDGKVLEFLRKLREQYGSMIGARYGEGFLTD
                     EPVPFELDVFLRTPAWAAGGAGTKGWNEPLGRRPVQRR"
     gene            complement(2977..4077)
                     /locus_tag="VFH83_01685"
     CDS             complement(2977..4077)
                     /locus_tag="VFH83_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485098.1"
                     /translation="MNNPRARQTAATAFAVLACALGCALLAGCSGNRPVPPAEAGGWY
                     QIQAGAFQRVASPSASLAVRFLPWTVQARVADMAYLGDTLYCGINRWGLVSVAVSETG
                     TPTFSYRFDALLFPHRTITALLPRRDRLTVHLYYNSVLNDASPGELANKSVSLVTFVP
                     QRSEFSLLTPPFQRQNPGWEAVGMAAVSQDEYILEWKQADSLQTQFAYTRFRADTLQE
                     TASDQEAFRSALGIPSISGPLVPNPRASLFAAAQTQVSGQAPATALVFSVRSRQAPLT
                     VSYRQVDSADAFVTIPVFEDGERRLALLPDGRVLTTRQDGALRTMTLPALPSGFHYTD
                     LVENAGWLIVAWEEAQFTDVGRAGVLVVRSTL"
     gene            complement(4074..>5447)
                     /gene="proS"
                     /locus_tag="VFH83_01690"
     CDS             complement(4074..>5447)
                     /gene="proS"
                     /locus_tag="VFH83_01690"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET6485099.1"
                     /translation="VLQAKLADYAPVKGCMVIRPRGYALWERMQRALDDMFKETGHEN
                     AYFPLLIPESFLKREAEHVEGFAPELAVVTHGGGEKLEEPLVVRPTSETIIWSMYKNW
                     IQSYRELPLLINQWANVVRWEKRTRLFLRTAEFLWQEGHTAHATAAEAEEETLRMLGV
                     YRQFAEEYMAVPVLHGVKTDSEKFAGAVRTYCIEAMMQDRKSLQAGTSHNLGQNFAKA
                     FDVTYQTNDKKLDYVWATSWGASTRLIGALIMAHSDDKGLVLPPRLAPTQVVIVPIFK
                     NETREAVLKFARGLRDALAPSLRVVLDEDDQATPGWKFSEYELQGIPIRVEVGPRDME
                     KGQVVAVRRDTGEKVFLPVAEAPQRLPSMLEAIQKSLFDRALKFRKENTFRTNDYAQF
                     TKLLDEPGGFIEAGWCGDAACEARVKEETKATIRLIPLEPQDTEGAPCMVCGKKASVI
                     AVFGRAY"
BASE COUNT          919 a         1804 c         1826 g          898 t
ORIGIN      
        1 gaacggatgt acgtccgaaa cagggactga atgacctgct cccggccccc ctccgagtag
       61 cgcacccgcg cgttgaagat cttgtgcttg cagtgctctg accaggtctg ggcgatcatc
      121 tccagctcca cgtccgtggc cgcggtggaa agcccatgcg cccttcggtc ctctcgaaca
      181 tcctctcgag cgtaatagcg ctgcacggcc agcatctctt cggcggagag cgccaggagt
      241 cgctcccggg aaagcgcgct caacgaatcc gcggccagtc cctccagctc gatcaggcga
      301 acctgggaag ggcttggcgc gatgctgccg gcgtagctct gcggcgggcg ggtgccttcc
      361 ctccactggg acctcgtgat gcatttcgcg gactggatca ggggattgtg aaagaaccgg
      421 gcaagctcgc cggggtccag ggccgcctgc cctcccccgt ccaccaggta ctgcaccgcg
      481 gtctggatgg aggcgctctc cgccgaatcc tccaggcaga ggcgcagcgc ctctcgggcg
      541 gtgagggcta cgggatccgt gacgccgggc cgaaccgtga cctccaccag gtagtcccag
      601 cccggggaat cctccgccgc gaaccggtcc accaggagcc gttgcgagac gccgtcacag
      661 aacacctctt cggcaaggtg aggggtaagg gccggcgcgc cctggatgag gaagacgtcc
      721 acgatggata cggcgcggat tcccaaggac acgtcccgcg ccaggctcgg caggagccgg
      781 cgcgccctgc cgtcggggaa ctccgggcgg ttgacgactt ccaggcgcac gaggggcccc
      841 cggctcagcg ggccgggtcc tccgatggat cgaaggagag ggtgaggagg cacatgtcgt
      901 ccaggggcgg ctcccctccc aggaactccc ggacggaagc catgagacgg tccagatcca
      961 ggaccccggc ttccccgatg atccgctgcg cctccgtcat cagccgttcc atgccgaagc
     1021 tttccacgcc tcggaccgcc tcgatcacgc catcgctcac gaagaggaac ccgtccccgg
     1081 aggcgaaggt ggtgtggctg gtctggtact cgtgcccgcg cacgtggccg atggggaaat
     1141 ccgaaaggtc gaacaggcgt tgagcggaac ccctggagat gaccaaaggc ggcacgtgga
     1201 ttcccgcgga ggagtactcg aaggtgagct gacgcgtgtc caggacgccc aggaagagcg
     1261 tgaagtagat ctccggatcg ctctggctga tctcctgcat gaaactctcg ttgatggagg
     1321 tgaggatggc gccgggcggc ttcaccacgt actggcggtt gatgaacatc ctcttgaacg
     1381 aggtctcgat cagcttcttg acgtagctca tgaccatggc ggcgtagacc ccatggccgc
     1441 agatgtctcc gccgtagatc cccagccgcc gctcgtcgat ctggtagacc cccgtgatgt
     1501 ccccgcccag ctcgtcacag ggcagatact cggcggccac cgtgacccca gggtagctct
     1561 tgcgccgctc gggaagcagg ttccgctgga tgctctgggc cagggccagc tcccggcgaa
     1621 tgcgacggtg ggagttctcg tactccaggg cgcgctcctc cgccttgcgc tccacgtcca
     1681 gccggtccag ccgaaggtct tcggcgagct ggtcgtttcg gcgcgccagg gagagggaca
     1741 cgttggccag gatccctcca gccagtcgag ggaaccggcg gagaagcgcc cccagctcta
     1801 cgccgtcggc gctgaggccc cgtacctcgg tcacggcgcg ggctacctgc cgccgcgcgg
     1861 gctgatcggg gaagaggctc cgctcgccga agaagcctcc ggcctcgacc tgagccagcg
     1921 ccacgcctcc cgtgatgggg tcactgagct ggacgcgacc ttcggagacg aggaagagcc
     1981 tgtccgcgga gccgacctgg gagatggtct cccccaccgc gaagcgctcc gcgcggaaga
     2041 gcccggccat ccgccgaacg tccgacgccg tgagaccgcg gacgagctgg tttccctcga
     2101 tcagccgtgc cagatctggt ttcatgatcg ttcgatgcag aatcgccgac cgacgcgccg
     2161 gtgtcaagtt ggtatcgttg gcgacgttgg cgctcgagcc cccgggcgcg cggccttcct
     2221 tgacccatcc caggcgggct catatcttga tcctgatggc ggatatcctg gtctttggcg
     2281 cgcacccgga cgacgcggag ttcgggatgg gcgcgagcat ggtgaagttc gttcgcacgg
     2341 gcgcctcggt ggcgttgtgc gtgctcaccc ggggagaggc cggcaccttc ggcaacggcg
     2401 agatgcggga gcaggagatg cgggaggccg cccgacggct gggcggagag atcgagatcc
     2461 tgtccttcag cgattgcgcc atcttcgaca cgtacgaggc acgggtcatc ctggcggagg
     2521 tgattcgccg ccaccgcccc aagctgatct tcgcccccta ccacaccaac ccgggcagtc
     2581 acaaggacgg cggggcgcac ccggaccact gggcaaccgg cgccatcgtc cggtcggcca
     2641 cgcggtacgc ccggttcgcc gggctcaagg aagtgaaggg cgagccctgg aatcccgagt
     2701 acgtcctgta ctacatgatg ccgcgcaacc gcgtgcccac cctggtgaac gacgtcagcg
     2761 agtacatcga ggagtgggag agcatcgccc gttcgcacca gtcccagctc gccatccggg
     2821 atggcaaggt gctggagttc ctgcggaagc tccgagagca gtatggctcg atgatcgggg
     2881 cgcgctacgg ggagggcttc ctgaccgacg agcccgttcc gttcgagctg gacgtcttcc
     2941 tgaggactcc ggcctgggca gccggcggcg cagggactaa agggtggaac gaaccactag
     3001 gacgccggcc cgtccaacgt cggtgaactg agcttcctcc caggccacga tcagccagcc
     3061 ggcgttctcc accagatccg tatagtgaaa gccggacgga agggccggca gcgtcatcgt
     3121 cctgagcgcc ccgtcctgcc tcgtcgtgag cacgcggccg tcggggagga gcgccagtct
     3181 gcgctcgccg tcctcgaaga cgggaatcgt gacgaaggcg tctgcggagt ccacctgccg
     3241 gtagctcacc gtgaggggag cctgtcgtga gcgcacggag aacaccaggg ccgtggccgg
     3301 cgcctggccc gagacctggg tctgagccgc ggcgaagagc gacgcacggg ggttggggac
     3361 cagcggaccg ctgatcgacg gtatccccag ggcggatcgg aaggcctcct ggtccgaggc
     3421 ggtctcctgg agcgtgtcgg cgcgaaatcg cgtataggca aactgcgtct gaagggagtc
     3481 cgcctgtttc cactccagga tgtactcgtc ctgggacacc gccgccatcc cgactgcctc
     3541 ccagcccggg ttctgccgtt ggaatggcgg cgtgagcagg gaaaactcgg agcgctgggg
     3601 cacgaacgtc acgagagaga cgctcttgtt ggccagctcg ccgggcgagg cgtcgttcag
     3661 cacggagttg taatagaggt ggaccgtgag ccggtcccgc cgaggcagga gcgccgtgat
     3721 ggtccgatgc gggaagagca acgcgtcgaa gcgataggag aacgttggcg tgcccgtctc
     3781 tgacacggcg acggacacca ggccccagcg gttgatgccg cagtagagcg tatccccaag
     3841 gtaggccatg tccgccacgc gtgcctggac cgtccacgga aggaagcgga ccgcgaggga
     3901 tgcggacgga gaggccacgc gctggaacgc gcctgcctgg atctggtacc agccgccggc
     3961 ctctgccggc gggacgggac ggttgcccga gcagcccgcc aggagcgcgc accccagcgc
     4021 gcacgccagc acggcgaacg cggtggcggc ggtctgccgg gccctcgggt tgttcaatac
     4081 gcgcgcccga agaccgcgat gaccgaggct ttctttccgc acaccatgca cggagcgcct
     4141 tccgtatcct gcggttccag aggaatcaga cggatcgtgg ccttggtttc ctccttgacc
     4201 cgggcttcac aggcggcatc cccgcaccag ccggcctcga tgaagcctcc gggctcgtcc
     4261 agaagcttgg tgaactgggc gtaatcgttc gtgcggaagg tgttttcttt tcggaacttc
     4321 agggcccgat cgaacagcga cttctgaatg gcctccagca tggaagggag cctctgtggc
     4381 gcctcggcga ccggaaggaa aaccttctcc ccggtgtccc gtcgtacggc caccacctgc
     4441 cccttctcca tgtcgcgcgg gccgacctcc acgcggatgg ggatcccctg cagctcgtac
     4501 tcggagaact tccagccggg cgtggcctga tcgtcctcat ccaggaccac gcgcagggat
     4561 ggagccaggg cgtcccgcag cccacgggcg aacttcagga ccgcctcccg cgtctcgttc
     4621 ttgaagatgg gcacgatgac cacctgcgtg ggagccagcc ggggcggcag caccagcccc
     4681 ttgtcgtcgg agtgcgccat gatgagcgcg ccgatcagcc gcgtggatgc gccccaggag
     4741 gtggcccaca cgtagtccag cttcttgtcg ttggtctggt aggtgacgtc gaatgccttg
     4801 gcgaagttct gacccaggtt gtgcgaggta ccggcctgga gggacttgcg gtcctgcatc
     4861 atcgcctcga tgcagtacgt gcgcaccgcg ccggcgaact tctccgagtc cgtcttcacg
     4921 ccgtggagga ccggcaccgc catgtactcc tccgcgaact gccggtacac gcccagcatc
     4981 cgcagggtct cctcctccgc ctcggccgcg gtggcgtgcg ccgtgtgtcc ttcctgccag
     5041 aggaactccg cggtgcggag gaagagcctc gtccgctttt cccagcggac cacgtttgcc
     5101 cattgattga tcaaaagcgg cagttcgcgg tacgactgta tccagttttt gtacatgctc
     5161 cagatgatcg tctccgaggt cggacggacg acgagcggct cctcgagctt ttctccgccg
     5221 ccgtgcgtga cgacggcgag ctcgggcgcg aaaccctcga catgttcggc ttcacgcttc
     5281 aggaagcttt ccggaatcag gagcgggaaa tacgcgttct cgtgccccgt ttccttgaac
     5341 atgtcgtcca gggcgcgctg catccgctcc cagagcgcgt agccgcgcgg tctgatgacc
     5401 atgcagccct tgaccggcgc gtaatccgcc agcttcgcct gtagaac
//
LOCUS       DASRZS010000070         3052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3503810, whole genome shotgun sequence.
ACCESSION   DASRZS010000070 DASRZS010000000
VERSION     DASRZS010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3503810"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VFH83_01695"
     CDS             complement(<1..856)
                     /locus_tag="VFH83_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET6485100.1"
                     /translation="MAWDRMFEAFSADLGIDLGTCSTLVHVRGKGIVLCEPSVVAMER
                     GTKKVMAVGTEAKKMLWKTPGDIVAIRPLRDGVIADFEATEKMIRYFIEKVIARRWFV
                     KPRMVIGVPTCITEVEKRAVRDSAEQAGARDVYLIQESMAAAIGANIPILEPAGHMIC
                     DIGGGTTEISVISLGGMVVTNAIRVGGDEFDEAIIKHVRNVHNLIIGESTAENVKITI
                     GNASPDKKIEKMEIKGTDAITGLPRKLEMDSVEVREALQEPINTVIEEIKKTLGQTPP
                     ELAADIVER"
     gene            complement(880..1830)
                     /locus_tag="VFH83_01700"
     CDS             complement(880..1830)
                     /locus_tag="VFH83_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6485101.1"
                     /translation="MILHYDHYRTGQRKRGARTILIPVISVVLVIAIVLVLALHVPSR
                     LFGGGAVRQAPGRLPDLWKAGQYEAVIAAADSILREDPLSPAALTYRGFASFYRAVTE
                     SAGEERSALLDQTILSLRRARLVATPFGGEADYVLGKAYFLKGKYYYDLAISSMESSL
                     AKGYEQKDSNQYIGVAYAQLGEYEKALGYFLTALKADTSDMLLLTIGLTYYQMKRSND
                     AVDYFLRTLNKTQDKGIEEQARFKLAEIYSDRGDLFKAEEQLLDIAKIDTHSAEAHYQ
                     LGEVYAKMNDPVRARAEWRNALIIDPSHYGAKLRYYPAKR"
     gene            complement(1852..2310)
                     /gene="rnpB"
                     /locus_tag="VFH83_01705"
     ncRNA           complement(1852..2310)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFH83_01705"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2374..>3052)
                     /locus_tag="VFH83_01710"
     CDS             complement(2374..>3052)
                     /locus_tag="VFH83_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HET6485102.1"
                     /translation="MDKGATSSAAEARVADINQRLADAEHNREKYEGQMDAIKTQREY
                     EALDKEIKDATEKEQNLRKELQREQTVIEELKNALEKEQMMIQKQEEDVKEEQSKIKL
                     KIKEKNAELKKLQGDEKKITPGLDEDILFKFERIIRSKAGVGIVSLTKGVCTGCYMIL
                     PPQFVNTVHLGAKVEFCPYCSRILFYQEDEEGESLETAILDSETEEEEEEEEEEEEAP
                     EEEDEEL"
BASE COUNT          435 a         1018 c          943 g          656 t
ORIGIN      
        1 cgcgctcaac gatgtccgca gcgagctccg gtggcgtctg gcccagcgtc ttcttgatct
       61 cttcaataac cgtgttgatg ggctcctgga gggcctcgcg gacctccacg gaatccatct
      121 ccagcttgcg cggcaggccg gtgatggcgt ccgtgccctt gatctccatc ttctcgatct
      181 tcttgtcggg ggaggcgttg ccgatggtga tcttcacgtt ctccgccgtg gactccccga
      241 tgatcaggtt gtggacgttg cgcacgtgct tgatgatggc ctcgtcgaac tcgtccccac
      301 ccacgcggat ggcgttggtg accaccatgc cgcccaggga gatgacggag atctccgtgg
      361 tcccgccgcc gatgtcgcag atcatgtggc cggccggctc caggatgggg atgttggcgc
      421 cgatggcggc cgccatggac tcctggatca gatacacgtc ccgcgcgccc gcctgctcgg
      481 cggagtcccg cacggcgcgc ttctccacct cggtaatgca ggtcgggacg ccgatgacca
      541 tccggggctt cacgaaccag cggcgggcga tgaccttctc gatgaagtag cggatcatct
      601 tctccgtggc ctcgaagtcc gcgatgactc cgtcccgcag gggccggatg gccacgatgt
      661 ctcccggggt cttccagagc atcttcttgg cctcggtgcc cacggccatg accttcttgg
      721 ttccgcgttc catcgcgacg acggagggct cgcagaggac gatccccttg cctcggacgt
      781 gcaccagagt gctgcaggtg ccgaggtcga tccccaggtc ggccgaaaac gcttcgaaca
      841 ttctatccca ggccatcagg gtaacctctc cttccgtgcc taccgcttgg cggggtagta
      901 gcggagcttg gctccgtagt gtgagggatc gatgatcagg gcgttacgcc attccgcccg
      961 cgccctcacc gggtcgttca tcttcgcgta cacctcgccg agctggtagt gggcttcggc
     1021 ggaatgggtg tcgatcttgg cgatgtctag gagctgctcc tcagccttga acaggtcgcc
     1081 ccgatcagaa tatatctcag ccagcttgaa ccgcgcctgc tcctcgatcc ccttgtcctg
     1141 ggtcttgttc aaggtgcgca gaaaatagtc gaccgcatcg ttgctcctct tcatctggta
     1201 gtaagtcagc ccgattgtca agagcagcat gtcgctagta tcggctttca gagcggtcaa
     1261 gaagtagccc agggccttct catactcccc gagctgcgcg taggccaccc cgatgtactg
     1321 gttggagtcc ttctgttcat atcccttggc cagggaggac tccatgctgg agatggccag
     1381 atcgtagtag tacttcccct tcaggaagta cgccttgccc aggacgtagt ccgcctcgcc
     1441 cccgaaggga gtcgccacca gacgggccct ccgcagggag aggatggtct ggtccagcag
     1501 ggcggaacgc tcttcccccg cgctctccgt gaccgcgcgg tagaagctgg cgaacccccg
     1561 gtaggtcagg gcggccgggc tgagcggatc ctcacgaagg atggagtccg ccgccgcgat
     1621 gaccgcctcg tactgaccgg ccttccacag gtccggcagc cttccgggcg cctggcggac
     1681 cgcgccgccg ccgaacaggc gggacgggac gtgcagggcc aggaccagga cgatggcgat
     1741 cacgagaacg acggagatca ccgggatcag gatggttcgc gcgccgcgct tgcgctggcc
     1801 ggttcgataa tggtcgtagt gcaggatcat gccgttgcgc gattatatag aaaaaggggc
     1861 aggtcgataa gccgggttct gtcgagggtc accatctctc tgcggcggcg gtcgcccgcc
     1921 gcctccagca gcctacccgc aggatccgcc gaggtccgcc tcggcgtgtc gagcgactcc
     1981 tgctgctcgg ctttgctccg aacgaggttt accgtgcccc ggccgttgcc gtccgggcgg
     2041 tgggctctta ccccgccttt tcacccttcc ctcgacggaa gaccgacccg cccgttgccg
     2101 ggaggagggc ccccgcctct cacggagcgg cgcggagcgc cgccccgata agggggtctg
     2161 ttttctgtgg cactgtctgt gtcgacgctt gccgcaagcg ggaagcatcg gccccggggg
     2221 ttacccggcg ttccgctctg aggagcccgg actttcctcc caggcggggg acgtccgtcc
     2281 cacgccgaag gcggtgacct gacctgcccc tctcgcgcgt cggctcttcc cggcggggga
     2341 agtcggcgcc gacgcgcggg aggggccgga gctctacagc tcctcgtctt cctcctcggg
     2401 cgcctcttcc tcctcttcct cttcttcctc ctcttcctcg gtttcgctgt ccaggatggc
     2461 cgtctccagg ctctcccctt cctcgtcctc ctggtagaag aggatccggc tgcaatacgg
     2521 gcagaactcg accttggcgc ccaggtggac cgtgttgacg aactggggag ggagaatcat
     2581 gtagcacccg gtgcagaccc ccttggtgag ggacacgatc cccacgcctg ccttgctgcg
     2641 gatgatccgc tcgaacttga agaggatgtc ctcgtcgagc ccgggggtga tcttcttctc
     2701 gtccccctgg agcttcttga gctcggcgtt cttctccttg atcttgagct tgatcttgga
     2761 ctgctcctcc ttgacgtcct cttcctgctt ctggatcatc atctgctctt tttccagagc
     2821 gttcttgagc tcctcgatca cggtctgctc ccgttggagc tccttgcgga ggttctgctc
     2881 cttctccgtg gcgtccttga tctccttgtc cagggcctcg tactcccgct gggtcttgat
     2941 ggcgtccatc tggccctcgt acttctcgcg gttgtgctcg gcgtccgcca gcctctggtt
     3001 gatgtccgcc acgcgcgcct cggcggccga gctggtggct cccttgtcca tg
//
LOCUS       DASRZS010000071         5297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4274735, whole genome shotgun sequence.
ACCESSION   DASRZS010000071 DASRZS010000000
VERSION     DASRZS010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4274735"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFH83_01715"
     CDS             <1..529
                     /locus_tag="VFH83_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485103.1"
                     /translation="GRWDNWLMRIADVLFSFPSLLLAIFVMAVLGEKTYNIVLAIGLV
                     YIPQFARISRGAILGIKTSDFVKAARASGAGSGSILFRHLLPNILVPLIVQVSLSLSV
                     AILLESSLSFLGLGVQPPAPSWGNMLSGARKVMVIAPWTALYPGMAIVLLVLGFNLLG
                     DGLRDILDPRLRNVR"
     gene            541..1581
                     /locus_tag="VFH83_01720"
     CDS             541..1581
                     /locus_tag="VFH83_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485104.1"
                     /translation="MDAERILEVEDLETQFRTEEGLVKAVDRVSFVLHRGETLGIVGE
                     SGCGKSVTNLSILQLLPARTARITGGRVLFRGRDLRSAREEQVRVVRGRNIAMIFQDP
                     LTSLNPLLRVWVQIAEALQLHQGMRRREARAKAVEMLKIIGIPDAERRANDYPHQFSG
                     GMRQRVMIAMALSCNPDILIADEPTTALDVTIQAQILDLISSLSARNSTAVILITHDL
                     GVVAGLCQTVCVMYAGHIVEKASVDDLFSDPLHPYTRGLLASIPRVDRAASVGSGGLG
                     EPGGSRRLTTIPGSPPSLIDLPDACPFHPRCPNAMDICRSAFPPERERPGRAPRTVSC
                     WLYAPKGDRAGP"
     gene            1568..2584
                     /locus_tag="VFH83_01725"
     CDS             1568..2584
                     /locus_tag="VFH83_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6485105.1"
                     /translation="MPDRDVLLEVRGLAVHFPIERGVILRRKVGVVRAVDGVDLTVRA
                     GEALGVVGESGCGKSTMAMAIAQLQRPTAGTVVFGGKNLGALSPRELREARMGFQIVF
                     QDPFSSLDPRMRVRDIIAEPLRLYARRGILPMDPAQIEARTRLLMERCGLSPSFGSRY
                     PHEFSGGQRQRIGIARALALGPKLVIADEPVSALDVSIQSQILNLLKDLQEELALTFL
                     FIAHDLAVIEYFCSRVAVMYLGRIVEIAESAVLYERPLHPYTKALLSAVPIPDPAKER
                     SRRRIILKGDVPSASRRIRGCPFRERCPEAMDRCAEIAPPLRQVEARHQVSCLLYDGV
                     GREE"
     gene            2581..3066
                     /locus_tag="VFH83_01730"
     CDS             2581..3066
                     /locus_tag="VFH83_01730"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6485106.1"
                     /translation="MSGGRITARSMRKDDAAACAGMACDSAIGERYGFTRETLCQTLT
                     SAIETGDDLFVAERDGKLAGFAWVDPRGAFSTAPYLRLIAVDPSVRGEGIGSALLAEF
                     ESRTLSVGRDWCLLVSDFNTQAQTFYEAHGYRKAGALPDFARPGITEILMVKKRAGRE
                     A"
     gene            3063..4145
                     /locus_tag="VFH83_01735"
     CDS             3063..4145
                     /locus_tag="VFH83_01735"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6485107.1"
                     /translation="MSTVAEERIRQITGRFLEEGYFRGAVLRVERDGRLLYEGAWGHA
                     LWTETERIAAGPSTLFDLASVSKLFTTTAILRLVTLGRFSLGDPVVALLRPSGGEAGL
                     ERALGNLDVASLLAHSSGILAWYPFYLRRGRPFEEVLADVLEANPPRRAVIYSDLNFM
                     ILGRIIEIATNLPLAAAMDTLVLEPLALDRTTYGAPAAMSAATELGNRIERSMVAERG
                     LSFEGWRDESRPIRGEPNDGNCYYFFGGTAGHAGLFSDARDLCRLGSLYRQRGQVEGA
                     PFLAHGLAEEAMRDHGGSRGLGFQGGEGYPGGGWGHTGFTGTWLYVSTDRDLVIALLT
                     NRLHVPQPRDISAYRREIVLALLAGT"
     gene            complement(4159..4617)
                     /locus_tag="VFH83_01740"
     CDS             complement(4159..4617)
                     /locus_tag="VFH83_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485108.1"
                     /translation="MPFAKTYRFTMQIPEAGRSLGGFLVESSDVSHDERGDGTIRYPV
                     TLVLVGPGGKSAVRKEVSERFGRGRTTFSGFGNPYQLQFGRFEVQSLGARRYRVTAVG
                     TGARIDLERELRRFVAYARARGEAPDDALLTAYLEDYRRDVTRRNPELAY"
     gene            4750..>5297
                     /locus_tag="VFH83_01745"
     CDS             4750..>5297
                     /locus_tag="VFH83_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6485109.1"
                     /translation="MAGRKWRIAGINFDHGHMGDNLRMAFNHPQAEIVGICHEERAAM
                     DRAVANFSIPATRVFTDYRRCLEETKPDIVLLCPATARHAEWVLKVAPYGVHVILEKP
                     FAASLADADRMIGAMRESGKLFAINWPLAWYPPHVTAKRLIDDGAIGEVLEVHYYDGN
                     RGPVFHTADKVELSAEERAREKA"
BASE COUNT          823 a         1785 c         1776 g          913 t
ORIGIN      
        1 cgggcgatgg gacaactggc tcatgcggat cgccgacgtg ctcttctcgt ttccctcgtt
       61 gctcctggcg atcttcgtga tggccgtcct gggcgagaag acgtacaaca tcgtcctggc
      121 catcgggctg gtctacatcc cccagttcgc ccggatcagc cgcggcgcca tcctggggat
      181 caagacgagc gacttcgtga aagcggctcg cgcgagcggc gcgggctctg gctccatcct
      241 ctttcgccac ctgcttccca acatcctggt gcccctcatc gtccaggtct ccctttccct
      301 gtcagtggcc atccttctgg aatcctccct cagctttctc ggcctggggg ttcagccgcc
      361 ggcgccgtcg tggggcaata tgctgagcgg ggcgcgcaag gtcatggtca tcgctccctg
      421 gacggcgctc tacccgggga tggccatcgt gctcctggtc ctggggttca acctcctcgg
      481 cgacggcctg cgcgatatcc tggacccgcg ccttcgaaac gtgcgttgat ggaaacggtc
      541 atggacgccg aacgcatcct ggaagtggag gacctggaga cgcaattccg gaccgaggaa
      601 gggctggtga aggcggtgga ccgcgtctcg ttcgtcctcc atcgcggaga gacgctcggc
      661 atcgtcgggg agtccggctg cggcaagtcg gtgaccaacc tgtccatcct ccagctcctt
      721 cccgcgcgca ccgccaggat cacgggcggt cgggtgctct tccgcggccg ggatctccga
      781 agcgcccgag aggagcaggt gcgggtcgtc cggggacgga acatcgccat gatctttcag
      841 gatcccctca cctccctcaa tccgctcctg agggtctggg tccagatcgc cgaggctctg
      901 cagctccacc aggggatgag gaggcgcgag gcccgggcca aggccgtgga gatgctcaag
      961 atcatcggca tacccgacgc cgagcgccgg gcgaacgact atccgcatca gttctccgga
     1021 ggcatgcggc agcgggtcat gatcgccatg gccctctcct gcaaccccga catcctgatc
     1081 gccgatgagc cgaccaccgc gctggatgtc acgatccagg cgcagatcct ggacctcatc
     1141 tcgagcctct ccgcgcgcaa ctccacggcc gtcatcctga tcacccatga cctgggcgtg
     1201 gtggccggcc tctgccagac cgtctgcgtc atgtacgcgg ggcacattgt ggagaaggcg
     1261 tcggtcgatg acctcttctc ggaccccctc cacccctaca cccggggact ccttgcctcc
     1321 atcccgcgcg tggatcgcgc ggcgagcgtg ggatcgggtg ggttgggcga gccaggtggg
     1381 tcacgccggc tcacgacgat cccgggctcc ccgccgagcc tcatcgacct gccggacgca
     1441 tgccccttcc atcctcgatg cccgaacgcc atggacatct gccgttcggc gttcccgcca
     1501 gagcgggagc ggccgggccg agctccccgg accgtgagct gctggctgta cgcgccaaag
     1561 ggcgatcgtg ccggaccgtg acgtcctcct cgaggtccgc gggctcgcgg tgcacttccc
     1621 gatcgagagg ggcgtcatcc ttcggcgaaa ggtgggagtc gtgcgcgccg tggacggagt
     1681 cgacctcacg gtccgcgcag gggaggcgtt gggtgtcgtc ggtgagtccg gctgcggcaa
     1741 gtccaccatg gccatggcca tagcgcagct ccagcgcccc acggctggca cggtcgtgtt
     1801 cggcggaaag aatctgggag ccctttctcc gcgcgagctt cgggaggcgc ggatgggctt
     1861 ccagatcgtg ttccaggatc cgttctcctc cctggacccg cggatgcggg tgcgggacat
     1921 catcgcggag ccgcttcgtc tctacgcgcg ccggggcatc ctccccatgg accccgcgca
     1981 gatcgaggcg cgcacccgcc tcctgatgga gcgctgcggg ctctctccct cgttcggctc
     2041 gcgctatccc cacgagttct ccggcggcca gcgccagcgg atcgggattg cgcgcgccct
     2101 ggcgctgggg cccaagctgg tgatcgcgga cgagcccgtg tccgcgctgg acgtgtccat
     2161 ccagtcccag atcctcaacc tcctcaaaga cctccaggag gagctcgccc tcacgttcct
     2221 cttcatcgcc catgacctgg ccgtgatcga gtacttctgc tccagggtgg ccgtcatgta
     2281 tctcggccga atcgtggaga tcgcggagtc cgcggtgttg tacgagcggc cgctccatcc
     2341 gtacacgaag gcgctcctgt ccgcggttcc catcccggac ccggccaagg agcgaagccg
     2401 tcggcggatc atcctgaagg gtgacgtccc ctcggcgagc cgacggatcc gtggatgccc
     2461 ctttcgggaa cgatgccccg aggccatgga ccgctgcgcg gagatcgcgc ccccgttgag
     2521 gcaggtcgag gcacggcacc aggtctcctg cttgctctac gatggcgttg ggagggagga
     2581 atgagcggag gccggatcac ggcgcggtcc atgaggaagg acgatgccgc cgcctgcgcc
     2641 ggtatggcgt gcgactccgc catcggtgag cgctacgggt tcacgcggga gaccctctgc
     2701 cagaccctga cctcagcgat cgagaccggg gacgacctct tcgtcgcgga gcgagatggg
     2761 aagctggccg gcttcgcctg ggtggatccg cgcggggcct tttccaccgc tccctacctg
     2821 cgcctcatcg cggtggatcc atccgtccgg ggggagggaa tcggttcggc gctcctggcg
     2881 gagttcgagt cccgcacgct ctcggtgggc agggactggt gcctgctcgt gtcggatttc
     2941 aacacgcagg cgcagacctt ctacgaggcg cacgggtatc ggaaggcggg cgccctgccc
     3001 gacttcgcgc gaccggggat cacggagatc ctcatggtga agaagcgcgc cggtcgggaa
     3061 gcatgagcac ggtcgccgag gagcgcatcc ggcagatcac cggccgcttc cttgaagaag
     3121 gctactttcg aggggcggtg ctccgcgtgg agcgcgacgg ccgtctcctc tacgaggggg
     3181 cgtggggcca cgccctgtgg accgagaccg aacggatagc ggcagggccg tccaccctct
     3241 tcgacctggc atcggtctcc aagctcttta ccactactgc cattctgcga ctcgtaacct
     3301 tggggcggtt ctcccttggc gatcctgtcg tggcgctcct gcgaccttca ggcggagaag
     3361 ccgggctgga gcgtgccctc gggaacctgg acgtggcgtc cctcctggcg cactcctccg
     3421 gcatccttgc ctggtacccc ttctacctgc gccgaggacg gccgttcgag gaggtgcttg
     3481 ccgacgtcct cgaggccaat ccaccgcgcc gtgcggtgat ctactccgac ctcaacttca
     3541 tgattctggg acggatcatc gagattgcca cgaacctgcc gcttgccgcg gccatggaca
     3601 ccctcgttct cgaacccctg gcactggacc gcacgacgta cggtgcgccc gccgcgatgt
     3661 ccgcggccac ggagctcggc aaccgcatcg agcgctccat ggtggcggag cgcggcctga
     3721 gcttcgaggg ctggcgcgac gaatcgagac ccatccgcgg agagccgaat gatgggaact
     3781 gctactattt cttcggcggc acggcgggtc acgccggtct gttctccgac gcccgcgatc
     3841 tctgtcggct ggggagcttg taccgccaga ggggccaggt ggagggcgcg ccgtttctcg
     3901 cgcacgggct tgcggaagag gctatgcgcg accacggagg ctcgcggggg ctcgggttcc
     3961 agggcggaga gggctacccc ggcggcggct ggggccacac gggctttacg ggcacctggt
     4021 tgtacgtgag cacggaccgg gatctcgtga tcgccctgct gaccaaccgt cttcacgttc
     4081 cccagccgcg cgacatctcc gcgtaccggc gcgagatcgt cctggcgctg ctggccggca
     4141 catagccatt gcgtcccgtc agtaggccag ctcggggttc ctccgggtca cgtcgcgtcg
     4201 atagtcttcc aggtacgcgg tgagaagcgc gtcgtctggc gcttcacccc gggcgcgggc
     4261 atacgcgacg aatcgccgca gctcgcgctc caggtcgatc cgcgcgcccg tcccgacggc
     4321 ggtcacgcgg tagcgccggg cccccaggct ctgcacttcg aaccggccga actggagctg
     4381 gtaagggttg ccgaaaccgg agaaggtggt ccggcctcgg ccgaagcgct cgctcacttc
     4441 ctttcgaacg gcggactttc ctcccggacc gacgagcacc agagtgacag ggtaccggat
     4501 ggtcccgtcg ccgcgctcat cgtgcgacac atcgctcgat tcgacgagaa acccgccgag
     4561 ggagcggccg gcctcgggta tctgcatcgt gaaccggtac gttttggcga acggcatacg
     4621 tttctcctgc tcgacatcgt tcgatgctgg aaagcctatg ggataggacg aaccgccgca
     4681 agccatcggg tccgagcggg agttggcagg cgcagggatc catgggagac tggccgtgga
     4741 ggaacgatga tggctggcag gaaatggaga atcgccggga tcaacttcga ccacgggcac
     4801 atgggggaca acctgcgcat ggcgttcaac catccgcagg cggagatcgt cggcatatgc
     4861 cacgaagagc gggcagccat ggatcgagcc gtggccaact tctccatccc ggcgacgcgc
     4921 gtgttcaccg actaccgtcg gtgcctggag gagacgaagc cggacatcgt gctcctctgc
     4981 ccggccacgg cccggcacgc ggagtgggtg ctcaaggtgg ccccgtacgg ggtgcacgtg
     5041 atcctggaaa agccgttcgc cgcaagcctg gccgacgccg accggatgat cggcgccatg
     5101 cgggagagcg ggaagctctt cgcgatcaac tggccgctgg cctggtaccc tccgcacgtc
     5161 accgccaaac gtctgatcga cgacggagcg atcggagagg tgctcgaggt ccactactac
     5221 gacgggaacc ggggcccggt gtttcataca gccgacaagg tggagctgag cgccgaggag
     5281 cgggcccggg agaaagc
//
LOCUS       DASRZS010000072         2987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3504344, whole genome shotgun sequence.
ACCESSION   DASRZS010000072 DASRZS010000000
VERSION     DASRZS010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3504344"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..485)
                     /locus_tag="VFH83_01750"
     CDS             complement(123..485)
                     /locus_tag="VFH83_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485110.1"
                     /translation="MSDIVPRNTVSKQGVHGVVGVAGGIGTIILAGLNPVLGIVVGGV
                     MGVIGLAFARGTKSERVGGIITAALGAGTVLSGLGHLLRHGGSGLFSGLMYGAGILLV
                     GYGGVSLYRFFRNLRKRM"
     gene            611..1456
                     /locus_tag="VFH83_01755"
     CDS             611..1456
                     /locus_tag="VFH83_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485111.1"
                     /translation="MPPLAKRIALLVALFSLVIGLFLLGYNLQLISDPLKSTVVNLWP
                     LLLVIAGIMLVLDSSRKRAITRASGVRSRSYPISVSRDASELELKVQFAYGRLVLGAG
                     GDCIRLVTEQMDPAEPTITQDVVGERAEVSIAMSQPLFPSHFQLRNSWKLELTRDRPL
                     RMSLLLHEADLRMDLRGMDIESLELRSDSGVQEIRVGRPRKKLSGRIYSSGGTLSLLL
                     PARAFAWVKLLNPFCRVDYPQGDLERREDGSLITPARPDALGNVELSIDGPIRNLVLD
                     IDDEP"
     gene            1507..2112
                     /locus_tag="VFH83_01760"
     CDS             1507..2112
                     /locus_tag="VFH83_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485112.1"
                     /translation="MKRSSSVLLCSLLLAAVLSVAPLAAADNPPAQTSQYSLGDQTLT
                     INAGLFVPLFLLPTWTPLVGSSPPHLSLGGDGYLSWAAYVTPHVRLGIDVAGTFSFDP
                     NNNALLMLPFIAKVSYSFNAYPFEIPISLGVGMNVIKYTDLSTIDLLVKPGVALYWIY
                     DSSWSFGLNLGYWFDVQFDTNPANSRVGNFLDISLGALYHY"
     gene            2117..>2987
                     /locus_tag="VFH83_01765"
     CDS             2117..>2987
                     /locus_tag="VFH83_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485113.1"
                     /translation="MAMKLRLILVCLAAAAFVACSPNGGQIYSIIENEKQAVDSTLPS
                     PKTQTVANVVSTGSGGPYYVAMGAIFRGVAKSDGTIDWTGGTGGSAVAVGAPAAGASC
                     NAMAYYGGHLWGGFVTAGTVLGLYESTTVSGAETFAGAQAVTDPSVQGLQIALIQVAN
                     GNLFVVTATVSGTDYVYNLVFYNGTTWFPVTSLTGVSAPITGVGWDGTQYWAVSSGTL
                     QGTQTVAPVIYTGTAASPPAFTAYGAPGSYTIATTDIIHGLFASQTPLGIVILCTKNE
                     GVYYLYGGTWYHLT"
BASE COUNT          510 a         1036 c          876 g          565 t
ORIGIN      
        1 gacaacggcc tcgacctccc gtcccattcc gcccccagcg atgcgctcgg gccgacaggg
       61 aacccagtca gagtatcggt tgcgagggag actccgctga gcgctgacgc gcacgcgggt
      121 gtctacattc tcttgcgaag gttccggaag aaccggtaga gcgaaacgcc gccgtacccc
      181 accaggagga tacccgcgcc gtacatgagg ccggagaaca gaccgctgcc gccgtgacgc
      241 agtagatgcc cgagcccgga caggaccgtg ccggctccga gcgccgccgt gatgatgcct
      301 ccaacgcgct cggacttcgt gccgcgcgcg aaagcgagtc cgatgactcc catcactccg
      361 ccgacgacaa ttccgagtac cggattgagc ccggccagga tgatcgtgcc gattccgcca
      421 gccacaccca cgaccccgtg gactccctgc ttgctgaccg tgtttctcgg aactatgtcg
      481 ctcattcggc acctccgtac tcaaggccaa tgtacccgtt ggcacgagtg atgtccacca
      541 aggcagccga tggcctcgcg cggactcccg ttgagttgac atgccactcc aatgacccta
      601 gagtaccgcc atgccccccc tggccaagag gatcgctctt ctggtcgcgc tcttctcgct
      661 ggtgattggg cttttccttc tgggctacaa cctgcagctc atctccgacc cgctgaagag
      721 cacggtcgtg aatctctggc cgctgctgct cgtcattgcc gggatcatgc tcgtgctcga
      781 ttcctcccgc aaacgcgcca tcacccgggc atcgggtgtg cgaagccgca gttacccgat
      841 ctccgtctcg cgggacgcat cggagctgga gctcaaggtg cagttcgcct atggtcggct
      901 ggtcctgggc gccggcggtg actgcatccg gctggtcacg gagcagatgg accccgccga
      961 gccgaccatc acgcaggacg tggttggaga gcgcgccgag gtctccatcg ccatgagcca
     1021 gccgctcttt ccttcgcatt tccagctccg caattcctgg aagctggagc ttactcgcga
     1081 tcggcccctg cggatgagcc tcctccttca cgaggcggac ctgcgcatgg acctgcgcgg
     1141 gatggatatc gagagcctgg agctgcgaag cgactctgga gtccaggaga tccgcgtggg
     1201 acggccccgc aagaagctct ccggccgcat ctactcctcc gggggaacgc tcagccttct
     1261 cctgccggcg cgcgcgtttg cctgggtcaa gctgctgaac ccgttttgcc gcgtcgacta
     1321 tccccaggga gacctcgagc ggcgcgaaga tgggtcgctg atcactccgg cgcgccccga
     1381 cgctttggga aacgtggagc tctccatcga cgggccgatc cgcaatctcg tcctcgacat
     1441 cgacgacgag ccttgaagaa gccgcacggt gcatggtact ttctgtagaa caccaggaga
     1501 cagttcgtga aacgatcgtc gtccgtcctt ctctgctcgt tgctgctcgc cgccgtcctg
     1561 tccgtggcgc ccctggcggc cgctgacaac ccgccggccc agacctccca gtactccctc
     1621 ggtgaccaga cccttaccat caacgccggg ttgttcgtac ccctcttcct tctgccaacc
     1681 tggacgcccc tggttggctc ctctccaccg cacctttccc tcggcggcga cgggtatctg
     1741 agctgggccg cctatgtcac cccccacgtc cggctgggga tcgacgtggc cggtaccttc
     1801 agcttcgatc cgaacaacaa cgccctgctc atgctgccgt tcatcgccaa ggtttcgtac
     1861 agtttcaacg cctatccctt cgagatcccg atctccctgg gagtcggcat gaacgtgatc
     1921 aagtacaccg atctttccac gatcgacctg ctggtgaagc cgggcgtggc cttgtactgg
     1981 atctacgact cttcctggtc cttcgggctg aacctcggct actggttcga cgtgcagttc
     2041 gacaccaatc cagcgaactc acgcgtaggc aacttcctcg acatctcgct tggtgccctc
     2101 taccattact aaaggaatgg ctatgaagct gcgtctgatc ctggtctgcc tcgccgccgc
     2161 cgcgttcgtc gcctgttcgc ccaacggagg acagatctac tcgatcatcg agaacgagaa
     2221 gcaggcggtc gattccactc tgccctcccc caagacgcaa accgtcgcga acgtcgtaag
     2281 cacgggctcg ggcgggccct actacgtcgc catgggcgcg atcttccggg gtgtggccaa
     2341 gtccgatggt accatcgact ggaccggcgg aaccggcgga agcgcggtgg cagtcggcgc
     2401 gccggctgcg ggagcctcct gcaacgccat ggcgtactac ggcggacacc tatggggcgg
     2461 cttcgtcacc gcgggaaccg tgctgggact ctatgagtcg actacggtga gcggcgccga
     2521 gacgttcgcg ggcgcgcagg ccgtcacgga cccctcggtc caggggctgc agatcgcgct
     2581 catccaggtt gccaacggga acctcttcgt tgtgaccgcg accgtgtctg gcacggacta
     2641 cgtctacaac ctcgtcttct acaacgggac cacgtggttc cccgtgacga gcctgaccgg
     2701 cgtgtccgct cccataacgg gggtcggttg ggatggcacg cagtactggg cggtcagctc
     2761 tggcacgctc cagggaacgc agacggtcgc gccggtcatc tacaccggca ccgcggcctc
     2821 accgccggcg ttcaccgcat acggggcacc ggggtcctat acgatcgcca ccacggacat
     2881 catccacggg ctgttcgcca gccagacccc gctcgggatt gtcatcctct gcaccaagaa
     2941 cgagggtgtg tactacctct acggaggcac ctggtatcat ctgaccg
//
LOCUS       DASRZS010000073         3709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3890410, whole genome shotgun sequence.
ACCESSION   DASRZS010000073 DASRZS010000000
VERSION     DASRZS010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3890410"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFH83_01770"
     CDS             <1..547
                     /locus_tag="VFH83_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET6485114.1"
                     /translation="KGNVVLMTFIYTHCTDLCPFIALKVKDAYQLLGADAKNVVLVAV
                     TTDPKRDIPQVNAAYSRAVGLFDAWHFVTGAPDAVHAVWASYGIGVTVDSGTDAAAPA
                     KTVNQASPAEEEPEPTQGLSSSDLDTAGSIIQAFGGGYDVAHSAPFWIVDKKGMIRLE
                     MDANATPTDIVTNLREMLRQR"
     gene            complement(574..1788)
                     /locus_tag="VFH83_01775"
     CDS             complement(574..1788)
                     /locus_tag="VFH83_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6485115.1"
                     /translation="MSTLGREGRLLFATRIVRLYAYGLVSVILVLYLAATGMSSLRIG
                     LLLTLTLVGDTVISLAITTNADRVGRKGMLLVGSALMVFAGVLFALTQDFVLLLVGAT
                     VGVISPSGNEVGPFLALEQAGLSQIVSDQRRTAVFSWYNLAGSFATALGSLSGGGLVQ
                     VLGSRGAAPLASYRVVVLFYAFLGVVLLVLFLRLGPTIEAARGERSGAGRLGLHRSRR
                     MVFKLSALFSLDAFAGALVVQSIVAYWFQIRFAVQPAVLGGIFFGANLLAGVSALSAA
                     RLASRFGLINTMVFTHIPSNVLTILVPLMPTLPLAVGLLLLRFSISQMDVPTRQSFIM
                     AAVAPDERSAAAGVTGIARTTGASLSPVFTGPMLANPLLLGLPFFLAGGLKIVYDLLL
                     FRAFRRTEAHGP"
     gene            complement(1899..2285)
                     /locus_tag="VFH83_01780"
     CDS             complement(1899..2285)
                     /locus_tag="VFH83_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6485116.1"
                     /translation="MGKYLRPVPRAATTSDVFNAVAEPRRRRILSYLAAREQSVGEIA
                     EALRLEQPSVSKHLKVLRDVDLVRVRRVGRTRYYRTNADSLRPLHAWADTFRRHWQHQ
                     LRRIKGIAEESGQLERNRRHEPPPPE"
     gene            complement(2388..2549)
                     /locus_tag="VFH83_01785"
     CDS             complement(2388..2549)
                     /locus_tag="VFH83_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485117.1"
                     /translation="MRDFHGKKVLEFDGKVVKDDKGKKVTTIEEIQKVIEGDADVALV
                     AMWQFFVRK"
     gene            2836..3690
                     /locus_tag="VFH83_01790"
     CDS             2836..3690
                     /locus_tag="VFH83_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET6485118.1"
                     /translation="MQPLMTRRLDEMTSREVELYVQGGGDLIFVPFGPISGHGAFIPL
                     GIHAHWAQALSLLVAERANGLVHPPIFTCYAGATRSFRATGIFSITEQVSILKRVAIT
                     LKERGFTRVVLMASTTPEWFGGMVAAREVFDETEQPVWMLVGERLLELPEVKSLYDGY
                     PGNFGETQIELASLTVLGRPAVIRYPEWAKAKKPDDPDQPTEIAEDVDRLRKMGTIGF
                     RYYEEGNHGNHGNAGLTHNGMSDVDLAVKVLEKSADLVLPLLESYTRYLKWIKAQPLN
                     FIVPKENL"
BASE COUNT          703 a         1209 c         1218 g          579 t
ORIGIN      
        1 caaagggaac gtggtgctga tgacgttcat ctatacgcac tgcaccgacc tgtgcccgtt
       61 catcgccttg aaggtgaagg acgcctatca gctcctggga gcggacgcca agaacgtcgt
      121 tctggtcgcc gtcactaccg accccaagcg ggacatcccg caggtgaacg cggcctacag
      181 ccgcgcggtg ggcctctttg acgcatggca cttcgtcacg ggcgcgccgg acgccgtcca
      241 tgcggtctgg gcgagctacg ggatcggggt gaccgtggac agcgggaccg acgcggccgc
      301 gccggccaag accgtgaacc aggcctcccc ggcggaagag gaaccggagc ccacgcaagg
      361 tctctcctca tcggatctgg acaccgccgg ctccatcata caggcgttcg gcggcggtta
      421 cgacgtggcc cactcggcgc ccttctggat cgtcgacaag aagggaatga tccgcctgga
      481 gatggacgcc aacgccaccc ccacggacat cgtgaccaac ctgcgcgaaa tgctgcgcca
      541 gcggtagcgc cgcggttccc cggaagggga ggctcagggg ccgtgcgcct cggtcctgcg
      601 gaaggcgcga aacaagagca gatcgtagac gatcttcagg ccgccggcca ggaagaaggg
      661 caggcccagg aggagcgggt tggccagcat cggcccggta aacaccggtg agagagatgc
      721 gccggtcgtg cgggctatcc cggtcacccc tgctgccgcg gacctctcat ccggcgccac
      781 cgcggccatg atgaaggact gccgcgtcgg gacgtccatc tgcgagatgc tgaaccgcag
      841 caggagcaac ccgaccgcca gcggaagggt gggcatcagg ggaaccagga tcgtgagcac
      901 gttcgatgga atgtgggtga agaccatggt gttgatcagc ccgaaccggg aggccagccg
      961 cgccgcgctg agggcggata cgccggccag caggttggcg ccgaagaaga tgccgcccag
     1021 gaccgcgggc tgcacggcga aacggatctg gaaccagtag gcgacgatac tctgcaccac
     1081 gagggcgccg gcaaacgcgt ccagggaaaa gagcgcggac agcttgaata ccatccttcg
     1141 cgaccgatgg agaccgaggc ggccggcccc tgatctctcg ccccgggcag cctcgatggt
     1201 ggggcccagc cgcaggaaca gaaccaggag gacgacaccc agaaacgcgt agaagaggac
     1261 caccacgcgg taggacgcaa ggggggcagc cccccggctg ccgagcacct gcaccagccc
     1321 gccgccgctg agcgagccga gtgccgtggc gaaggaaccc gccaggttgt accaggagaa
     1381 gaccgcggtc cgcctctgat ccgatacgat ctgggacagc ccggcctgct ccagggccag
     1441 gaagggtccc acctcgtttc cgctggggct gatcacgccg accgtggcgc cgaccaggag
     1501 gaggacgaag tcctgcgtga gggcgaagag cacgccggcg aacaccatca gagctgagcc
     1561 caccagcaac attccctttc ggcccacccg gtcggcgttc gtcgtgatgg cgagagaaat
     1621 caccgtgtcg ccgacgagcg tcagcgtgag cagaaggccg atgcgcaggc tgctcatgcc
     1681 ggtggccgcc aggtacagca ccaggatgac ggaaaccaga ccgtacgcgt acagccgcac
     1741 gatcctcgtg gcgaacagaa gccggccttc gcgacccagg gtgctcaggt gcgcctcctc
     1801 agacccgtgg tgccggtgct tcgagctcga tcgtcagggt gaggtcttct atggaacgaa
     1861 gcgacacggg ttcctccttg ggaatcattc tggggaaatc attctggcgg aggcggctcg
     1921 tggcgcctgt tccgttcgag ctggccactc tcctccgcga tccctttgat tcggcggagc
     1981 tggtgctgcc agtgccgtcg gaacgtgtcg gcccacgcgt gcagcggccg gagggagtcg
     2041 gcgttggtcc ggtagtagcg ggtcctcccg acccgccgga ctcgcaccag gtccacgtcc
     2101 cgcaacacct tgaggtgctt ggagaccgat ggctgctcga gcctcaacgc ctcggcaatc
     2161 tcacccaccg actgctcccg cgccgccagg taggagagaa tcctgcgacg ccgaggttcg
     2221 gcgacggcgt tgaagacatc cgaggtcgta gcagcccgcg gcaccggccg aagatactta
     2281 cccatatggg aatatgtcaa acggaaagcg ttggcgcgcg ggtggggggg cggggcggct
     2341 ggcggagcgg accggcgccg accccgtggc ctgcgccggt cggagtctta cttcctcacg
     2401 aagaactgcc acatcgcgac cagcgcgacg tctgcatcac cctcaatgac cttctggatc
     2461 tcctcgatcg tcgtgacctt tttccctttg tcgtccttca ccacttttcc atcgaactcg
     2521 aggaccttct tcccgtggaa gtctcgcacg ttcttgccct cgatctcacc gagcttggcg
     2581 gcattcgcgc ctcggatgag gctcccgtcc agctagccca gccgctgccc ggagcttgtc
     2641 tttcggaatc gcttcccgtc aaaggtatag tcggccactg cgcgcctcct agtctgccct
     2701 aaccataccg ccggcagggt atagcgtcag gagtcgccct tgtgcgcgcc gtggctcgcg
     2761 tgtatccatt agggacgtag caagacagaa gggacagtcg aaccagggcc ggtcacgaca
     2821 cgaagggaga ccatcatgca accgttgatg acgcgcagac tggacgagat gaccagccgc
     2881 gaggtggagc tgtacgtcca ggggggcgga gacctgatct tcgttccctt tggtccgatc
     2941 tcagggcacg gggccttcat ccccctggga atccacgccc actgggccca ggcgctgtcc
     3001 ctgttggtgg cggagcgggc caacggcctg gtgcaccctc ccatcttcac ctgctacgcc
     3061 ggcgccaccc ggtccttccg cgccaccggg atcttttcca tcaccgagca ggtgtcgatc
     3121 ctgaagcgcg tcgccatcac cctcaaggag cgtgggttca cccgagtggt gctcatggcc
     3181 tccaccacgc cggagtggtt cggcggaatg gttgccgccc gcgaggtgtt cgacgagacg
     3241 gagcagccgg tctggatgct ggtgggggag cggctgctcg agctcccgga ggtgaaatcc
     3301 ctttacgacg gctaccccgg caacttcggg gagacgcaga tcgagcttgc cagcctcacg
     3361 gtcctgggga gacccgcggt catccgttac cccgaatggg ccaaggcgaa gaaacccgat
     3421 gatcccgacc agccgacgga gatcgccgaa gacgtggacc gcctgcgcaa gatggggacc
     3481 atcggctttc gctactacga ggaaggcaac cacggcaacc acggaaacgc gggtctgacg
     3541 cacaacggaa tgtcggatgt ggatctcgcc gtgaaggtgc tggaaaagag cgcggacctc
     3601 gtgttgccgc tgctggagag ttacacgcgc tacctgaagt ggatcaaggc gcagccgctc
     3661 aacttcatcg tcccaaagga gaacctgtag cccacacccg cccgttgcg
//
LOCUS       DASRZS010000074         8508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4661947, whole genome shotgun sequence.
ACCESSION   DASRZS010000074 DASRZS010000000
VERSION     DASRZS010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8508
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4661947"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..585)
                     /locus_tag="VFH83_01795"
     CDS             complement(64..585)
                     /locus_tag="VFH83_01795"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET6485119.1"
                     /translation="MRKRVAVVTLVVVLVLVAVGLDQLAKGMARGHLADGRTVFVAGR
                     VLVLRYVENPGAFLELGSRLPYPVRRVALIVIPLAILAWVVAYLASRRDTGTAAVIGL
                     SLVAGGGLGNLVDRLGANGRVSDFINVGIGNLRTGIFNLADLFIMSGCVILLIVELSR
                     RREPAGRSGGTEV"
     gene            757..2148
                     /locus_tag="VFH83_01800"
     CDS             757..2148
                     /locus_tag="VFH83_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HET6485120.1"
                     /translation="MQAKKATSSQRPTADQDPADGAATVELISEFARTYDANPLSTVR
                     QNALAESDASKLVLNQRVAMAIDRNFSTRLDDWGPTDQKSSGRCWLFAATNLLRAGVI
                     KRLKLKSFEFSQSWLMFWDKLEKANYFLEAIRDTADRDVDDRTVVTILGNCVNDGGQW
                     NMFVNIALKYGLVPKSAMPETLSSSSTGSMNLALTGRLRVAARALRALHAAGKPASDL
                     REEKRKALDDVYTILRLHLGNPPDRFDWQWTDDKKKFHRELGMTPKAFRKKYATLPLE
                     DYVCLVHDPRASSPFGRTFTVEYLGNVVEGGQVIYLNVEMATLKEIAMRSLKAGEPVW
                     FGCDCSKMMHRDLAVWDARMFDYEGLYGVPLELSSKEDRLLYKRTAMNHAMLFTGVDE
                     DHGKTRKWRVENSWGEKGDGKGFYVMNDSWFDEHLFEIAAPRSRLPAKLRAALSQAPI
                     VLPAWDPMGSLAR"
     gene            complement(2152..3030)
                     /locus_tag="VFH83_01805"
     CDS             complement(2152..3030)
                     /locus_tag="VFH83_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485121.1"
                     /translation="MKTTKLLDGLCVFIAAAGMVAATVGLFWNDGGQPFPFTTIHGSI
                     AHQYGTGLYRFDTVLIGAGFKGQDAVVLFLGVPLLVLATVFGRTGRLRARLLQTGILA
                     YFLYVYASMAFGAAYNTLFLLYVATFSASLFALYLAFSLTLELHSGYASRLPRLWPAV
                     LLLASGALTALIWLVPLVTAMASGGPPMLLDGYTTMLTDALDLGIVAPLAVVAGVLTL
                     RGSPMGFRLAFPILGLLVMLLPTIVGATVSQVGAGVAFTPGAIIGPVAGFLILGLLGL
                     WVLVTFLLRLDQGSRR"
     gene            3352..4581
                     /gene="tyrS"
                     /locus_tag="VFH83_01810"
     CDS             3352..4581
                     /gene="tyrS"
                     /locus_tag="VFH83_01810"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET6485122.1"
                     /translation="MEMEVREEVELLMQGTEYGDAELAARMASELEERVVQTRREGRP
                     LRVYCGFDPRTSDLHLGHMVPIRKLRQFQELGHEVTFLVGTGTALIGDPSDKTGLRAA
                     MSRQEAMENGRTYAEQAFRMLDPGKTRVRFNHEWLEGLRLEELSRLAAQFTIQQILSR
                     ENFHARWQRGDPVYLHETLYSLMQGYDAFTLQADVQVGGSDQLFNIVTASRRLMSALG
                     ARPNVALIVGILPGTDGSVKMSKSLGNHIRILASPQDMYGKVMSVPDTAMRSFFDLAT
                     PLPPADVAVLFRRVDSGLLHPRDLKMRLAREIVSAFHSPNEARTAEEEFVRVFQRSET
                     PQEVPELRIAGPVALTEALVSGGLLPSRSEARRLITQRGIRVDGVVVESDRMVEETCL
                     VQVGKRRFLRVSRGPSG"
     gene            4665..5270
                     /locus_tag="VFH83_01815"
     CDS             4665..5270
                     /locus_tag="VFH83_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HET6485123.1"
                     /translation="MDEQRSGGPISLGGRFLISEINLTDPNFYRSVILMVSHDDSGAF
                     GLVLNRRSRFTLGELLEEMEGSPAAALPVFVGGPVQQEALFVLHTDVSRFSEGGDSKS
                     PLPGVVFEPMARDTLDYLAKEWATRPEDDRPKIRIYAGYSGWGPGQVEGELKSEAWVV
                     IDGTQEMVFHPDPSKGWAEALASKGPLYQIYLQTGFKPSMN"
     gene            complement(5303..7198)
                     /locus_tag="VFH83_01820"
     CDS             complement(5303..7198)
                     /locus_tag="VFH83_01820"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HET6485124.1"
                     /translation="MTTPKTCYRHDYPIAPAPFTAVTLRDRFWAPRLETNRTVTIPSV
                     LRKCEESGRVDNFRRAGSSLPGPYVGKNPFDDTDVYKTLEGAARSLASHPDPELDAYV
                     DRLIELIARAQEPDGYLYTNRTIDPRSTHPDAGPERWSNLVMSHELYNCGHLYESACA
                     HHLATGKRSFLDVALRSADLVRREFGPNARHDIPGHQIIEMGLARLYRITGDRGYLET
                     ARFFLEQRGHHETRPLYQYEENEGYCQDHLPVLEQREAVGHAVRAVYMYSGMADVAAL
                     APDPAYARALDAIWRSVVESRIYLTGGLGSRHKGEAFGEAFELPNATAYAETCAAIGS
                     VQWNHRLFLLTGESRFYDVLEQTLYNRLLSGVSLKGDEFFYANPLESDGVFPFNQGAV
                     GRQPWFVVSCCPTNLSRFFPSLPGYLYATTETAVYVNLFAGSSARVEVAGQRVEVVQE
                     TDYPWSGSVRITVHPERKARWQLLVRIPGWARGRVLGGSLYRFEGRAPEPARILLNGK
                     PTELTERSGYASLDREWEDGDVVEIDLPMPVCKVIADSRIPDTRGKAAIQRGPIVYCV
                     EGMDADRPIAEVAIGGRASLEAHFEPDVLRGLVVVQGKGFRAVPYYAWANRGPGTMRV
                     WLRNSAG"
     gene            complement(7209..8036)
                     /locus_tag="VFH83_01825"
     CDS             complement(7209..8036)
                     /locus_tag="VFH83_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485125.1"
                     /translation="MRPERTNVALTVILSLLALACVFPFLWMVRSSFLSNIAINTYPP
                     QWIPKVWHFENYLETLRTFPFQRYLLNTLTITVPSLVGVLLTSTLAAYAFARLEFRGK
                     KFWFTMVVASMLMPGSVTIIPIFLGWTFLGLSSGFVPLIVPAFLGGGGFNIFLGRQFL
                     MTIPRDLDEAAWIDGAGHIRTLFQILLPLIKPVLISIFLFTFILYWNDLLGPLIYISR
                     NEQNTISQGLANFRAGFGTDYRAIMAACTMSIAPAVILYFIGQRYFIEGIVLSGLKA"
     gene            complement(8045..>8508)
                     /locus_tag="VFH83_01830"
     CDS             complement(8045..>8508)
                     /locus_tag="VFH83_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485126.1"
                     /translation="VGVAKVQWLGNDLTATPSIIALTVWGLGNFIVIFLAGLQGIPRS
                     YLEAVQIDGGNAWHRFRHVTLPLMSPIIFFNLLMSLVTNLQIFVPARALTKGGPNNST
                     LYMVLLIFREGFERNNFGHAAAVSLLFFLFIGALTGVIFATSRRWMFFEGK"
BASE COUNT         1613 a         2708 c         2835 g         1352 t
ORIGIN      
        1 cgtacaagac cccggttggg cgccttctgt caagcccgcg gcgtgtcggc tccgggcccg
       61 gggtcaaacc tcggttccgc cgctccgacc agccggctcc cggcgccggg agagctcgac
      121 gatcagcagg atgacgcagc cgctcatgat aaagaggtcc gcgaggttga agatcccggt
      181 gcgcaggttg ccgataccca cgttgatgaa atcgctcacc cgcccgttgg cgcccaggcg
      241 atccaccagg ttccccagcc cgcctcccgc caccagggag agcccgatca cggcagccgt
      301 gcccgtatcc cgacgactcg ccagataggc gacgacccag gccaggatgg cgagcggtat
      361 gacgatcagg gccacccgtc tgaccggata cggaagccgg gaacccaact ccagaaacgc
      421 gcccgggttc tcgacgtacc gcaggacgag aacccgaccg gccacgaaca cggtccgccc
      481 atccgccagg tgtccccggg ccatgccttt ggcaagctgg tccagcccga ccgccaccag
      541 aacgagaacg acgaccagcg tcacgaccgc aacgcgtttg cgcacggacc ctccccctcg
      601 atgaatcata cgccctcaga ggcaagcccg cataggcgcc gttcgcctgc gttcgacgag
      661 gcgtcggcga tgcggcccgg atcgactgcc cgcggttcgg atcttgaccg ctcccttcgc
      721 cctctctaga ctgccgctca cgtcaggagg cgtaccatgc aagccaagaa ggccacatcc
      781 tcgcagcggc cgacagcgga tcaggacccg gccgacggcg cggccaccgt ggagctcatc
      841 agcgagttcg cccgaaccta cgatgccaac ccgctctcga cggtgcgtca gaacgcgctg
      901 gcggagtccg acgcgagcaa gctcgtgctc aaccagaggg tggccatggc catcgaccgc
      961 aacttctcca cgcgtctgga cgactggggt ccgacggacc agaaaagctc ggggagatgc
     1021 tggctcttcg ccgccaccaa tctcctgcgc gccggggtca tcaagcggct gaagctcaag
     1081 agcttcgagt tcagccagag ctggctcatg ttctgggaca agctcgagaa ggccaactac
     1141 ttcctggagg ccatccggga cacggcggac cgcgacgtcg atgaccggac cgtggtcacc
     1201 atcctcggca actgcgtcaa cgacgggggc cagtggaaca tgttcgtgaa catcgcgctc
     1261 aagtacggcc tggtgcccaa gagcgcgatg ccggagacgc tcagctcctc ttcgaccgga
     1321 agcatgaacc tggcgctgac gggccgactc cgcgtggcgg cgcgggccct gcgcgccctg
     1381 cacgcagccg ggaaaccggc atcggatctg cgggaagaga aacgcaaggc tcttgacgac
     1441 gtctacacga tcctccgtct gcacctgggc aatccccccg accgtttcga ttggcagtgg
     1501 accgatgaca agaagaagtt ccaccgcgag ctcggcatga cccccaaggc gttccggaag
     1561 aagtacgcga cccttcccct ggaggactat gtctgcctgg tccacgatcc ccgcgcctcc
     1621 agcccctttg gccgcacctt caccgtggag tacctgggaa acgtcgtgga gggtggtcag
     1681 gtcatctatc tgaacgtcga aatggcgacc ctcaaggaga tcgccatgcg ctccctgaag
     1741 gcgggcgagc ccgtgtggtt cggctgcgac tgcagcaaga tgatgcaccg cgatctcgcc
     1801 gtgtgggacg cccgcatgtt cgactacgag ggtctctacg gtgtgcccct cgagctttcc
     1861 tccaaggagg accggctgct gtacaagcgg accgccatga accacgcgat gctcttcacc
     1921 ggcgtggacg aggatcacgg caagacccgc aagtggcggg tggagaacag ctggggggag
     1981 aagggcgacg gaaaggggtt ctacgtcatg aacgattcct ggttcgacga gcacctcttc
     2041 gagatcgcgg cgcccaggag caggttgccc gcgaagcttc gggctgccct ctcccaggcg
     2101 ccgatcgtcc tgccggcctg ggaccccatg ggctccctgg cgcgctgatc cctaccgacg
     2161 cgagccctgg tccagccgca ggaggaacgt gaccagcacc cagagcccaa ggaggcccag
     2221 gatcaggaac cccgccacgg gaccaatgat cgctcccggc gtgaatgcga caccggcacc
     2281 gacctggctc accgtggcgc cgacgatcgt cggcagcagc atgaccagca gtcccaggat
     2341 cgggaacgcc agacgaaagc ccatgggaga gccgcgaagg gtgaggacgc cggccacgac
     2401 ggccaggggc gcgacgatgc cgaggtccag cgcgtcggtg agcatggtgg tatatccgtc
     2461 taggagcatg ggaggtccgc cgcttgccat cgccgtgacg agggggacca gccagatgag
     2521 cgccgtgagc gcgccgctcg ccaggagcag cacggccggc cagaggcgag ggagccggct
     2581 cgcgtacccg gagtgcagct ccagggtgag cgagaaggcg agatacaacg caaagagact
     2641 cgcggagaag gtcgccacgt agagaaggaa caacgtgttg taggcggccc cgaacgccat
     2701 ggatgcgtag acgtagagga agtacgccag gatgccggtc tgcaggagac gggcccgaag
     2761 cctcccggtc cggccgaaca cggtcgccag aacgagcagc gggacaccaa gaaagagcac
     2821 tacggcgtcc tgtcctttga acccggctcc gatcagaacc gtgtcaaacc ggtacaagcc
     2881 ggtaccatac tggtgcgcaa tcgagccatg gatcgtggtg aacgggaacg gctggccgcc
     2941 gtcattccag aacaggccga ccgtggccgc caccattccc gcggcggcga tgaaaacgca
     3001 caggccatcc agaagctttg tggtcttcac ggggcacctc ccgccggaag ataccgcgtt
     3061 gccgatcgct gcgccatctc atcgccaatc tcgtctgcgc tgtcggcttg acatcggtcc
     3121 ttgtggcgtg caaccttccc tcaagcgagc gacaatcgaa tgacggtcag gatccgctac
     3181 tcctattatc atctcccgtt caggcgcccg aggacgtcgg gatgatggta tagcagccgc
     3241 gccatcatcg cccccgggcc agaagcccgg gggctttttt ttggtgcgga gccgaagtgc
     3301 cgcccgtccg tcaccccagc ggcaacgacc gcggaggaga ggaggaaggt catggagatg
     3361 gaggtccggg aagaggtcga gcttctcatg cagggcaccg aatacgggga tgcagagctg
     3421 gccgcccgca tggcgagcga gctggaggag cgggtcgtcc agacccgtcg cgaggggcgc
     3481 cccctgcggg tgtactgtgg attcgacccc cgcacgtccg acctccacct cggtcacatg
     3541 gtccccatcc gcaagctgcg ccagttccag gagctggggc atgaggtgac gttcctggta
     3601 ggcacgggca ctgccctgat cggagacccg tcggacaaga ccggccttcg cgccgccatg
     3661 agccgacaag aggccatgga gaacggcagg acctacgccg agcaggcatt tcggatgctg
     3721 gatcctggaa agacccgggt ccggttcaac cacgagtggc tggagggcct gcggctggag
     3781 gagctgtccc ggctagcggc tcagttcacg atccagcaga tcttgagccg tgagaacttc
     3841 cacgcgcgct ggcagcgagg agacccggtg tacctgcacg agaccctcta ttccctgatg
     3901 caaggctacg acgcgttcac cctccaggcg gacgtccagg tcggcggcag cgaccagctc
     3961 ttcaacatcg tgacggcctc ccgcaggctc atgagcgcgc taggagccag acccaatgtc
     4021 gcgctcatcg tcgggatcct ccccggcacc gacggaagtg tgaagatgtc caagtcgctg
     4081 gggaaccaca tccggatcct cgcctcgcct caggacatgt acggtaaggt gatgagcgta
     4141 ccggataccg ccatgcggtc gttctttgat ctggccaccc ctctgccgcc ggccgacgtt
     4201 gcggttctgt tccgtcgggt cgattcgggg ctgctccatc ctcgggacct gaagatgcgc
     4261 ctggcccggg agatcgtctc cgctttccat tccccgaacg aggcccgaac ggcggaagag
     4321 gagttcgtcc gcgtgtttca gcggagcgag actccccaag aggtccccga gctgcggatc
     4381 gcggggcctg tggcgctgac ggaagctctg gtgtcgggcg gtctgctgcc gtcgcggagc
     4441 gaagcccgca ggcttatcac gcagcgcggg atccgcgtgg atggcgtcgt cgtagaatcg
     4501 gaccggatgg tcgaggagac gtgtctcgtc caggtgggca agcgccgatt cctccgcgtg
     4561 agccgcgggc cctcgggctg acgactgggg ccggcgtgag cgtgtcgccg caaacccggg
     4621 cccgtggttg ccggtccagg gggactgcac tacactttcg ggccatggac gagcagcgca
     4681 gcggcggacc gatttccctc ggcggccggt tcctcatatc ggaaatcaat ctcacggatc
     4741 cgaacttcta ccgctcggtg attctcatgg tatcccacga cgatagcggg gcattcgggc
     4801 tcgtgctgaa ccggcgctcc cggttcaccc tcggtgagct gctggaggag atggagggaa
     4861 gcccggcggc ggccttgccg gtgttcgttg gggggccggt ccagcaggaa gcgctgttcg
     4921 tgctgcacac ggacgtctcg cgcttctccg agggcggtga cagcaagtcg ccgctgcccg
     4981 gcgtcgtctt cgagcccatg gcgcgcgaca ccctggacta cctggccaag gaatgggcga
     5041 ccaggcccga ggatgatcga cccaagatcc ggatctacgc cgggtactcc ggctggggac
     5101 cgggccaggt cgagggagag ctcaagtccg aagcctgggt cgtgatcgac ggcacgcagg
     5161 aaatggtctt tcatcccgac ccgtccaaag gctgggcgga ggccctcgcc agcaagggcc
     5221 ccctgtacca gatctacctg cagacagggt tcaagccgtc gatgaactga cgccctccgc
     5281 ggggtcgact gcccggcgag gctcagccgg cggagtttcg cagccagact ctcatggttc
     5341 cgggaccacg attggcccag gcatagtagg gcacggcccg gaaccccttc ccctggacca
     5401 caaccaggcc tcgcagaacg tcgggctcaa agtgcgcctc cagcgatgcc cggcccccga
     5461 tcgccacttc ggcgatcggc ctatccgcgt ccatcccttc cacgcagtag acgatgggcc
     5521 cgcgctggat ggcggccttc ccccgagtgt ccgggatccg cgagtcggcg atgaccttgc
     5581 agacgggcat ggggaggtcg atctctacga catctccgtc ttcccactcc cgatccagag
     5641 aggcgtagcc gctgcgctcg gtcaactcgg tgggctttcc gttcaagagg atgcgcgcgg
     5701 gctctggggc gcgcccctcg aaccggtaga gggatccgcc caggaccctt cctcgggccc
     5761 agccggggat ccgaaccagg agctgccatc gggccttgcg ctcagggtga accgtgatcc
     5821 gcaccgatcc gctccaggga tagtccgtct cctggaccac ctccacccgc tggcccgcca
     5881 cttcgacccg ggcgctgcta ccggcaaaca gattgacgta caccgcggtt tccgttgtgg
     5941 cgtagaggta gcccggcagg gacgggaaga accgggaaag gtttgtagga cagcaggaga
     6001 ctacaaacca cggttgtcgg cccacggcgc cctggttgaa cgggaagact ccgtccgatt
     6061 ccagcgggtt ggcgtagaag aactcgtctc ctttcagaga gacgcccgag agaagcctgt
     6121 tgtacagggt ctgctccaac acatcataga accgtgactc gccggtgagg agaaacaggc
     6181 ggtggttcca ctggaccgaa ccgatggccg cgcacgtctc ggcgtatgcc gtggcgttgg
     6241 gaagctcgaa tgcctcccca aacgcctcgc ctttatgccg cgagcccaga cctcccgtga
     6301 ggtagatccg cgactcgacc acgctccgcc agatggcatc gagggctcgc gcatacgcgg
     6361 gatccggagc cagcgccgcg acatccgcca tgccgctgta catataaacg gctcgcaccg
     6421 cgtgcccaac ggcctcccgc tgctcgagca cggggagatg atcctggcag tacccctcgt
     6481 tctcctcata ctggtagagc ggtcgtgttt cgtggtgccc ccgctgctcc aggaagaagc
     6541 gcgcggtctc caggtagcct cggtcgccgg tgatccggta cagccgcgcc agccccatct
     6601 cgatgatctg gtggccggga atgtcgtggc gagcgttggg gccgaactcc cgtcggacca
     6661 ggtccgcgct tcgaagcgca acgtcaagga aggaccgctt gccagttgcc aggtgatggg
     6721 cgcaggcgga ctcgtacaaa tggccgcagt tgtacagctc gtggctcatg acgaggttgg
     6781 accatctctc gggacccgcg tcggggtggg ttgagcgagg gtcgatggtc cggttggtgt
     6841 acaggtagcc gtcaggctcc tgcgcccgtg cgatgagctc gatcagacgg tccacgtagg
     6901 cgtccagctc ggggtccggg tgagaagcca gggagcgggc ggcgccctcc agggtcttgt
     6961 acacatccgt gtcatcgaac ggattcttgc cgacgtacgg ccctggcaga ctgctgccgg
     7021 cgcgtcggaa gttgtccacc ctccccgact cctcgcactt tcgcaagacc gaggggatgg
     7081 tgaccgtccg gttcgtttcc agccgcggag cccagaatcg gtcccgcagg gtcaccgcgg
     7141 tgaacggggc cggcgcgatg gggtagtcgt gtcggtagca ggtcttcggt gtggtcatgg
     7201 gtgcctcctt acgccttcag acccgagagc acgatgcctt cgatgaagta gcgctggccg
     7261 atgaagtaca ggatcaccgc aggcgcaatc gacatcgtgc acgcggccat gatggcgcgg
     7321 tagtcggtcc cgaagccggc ccggaagttg gccagcccct gggagatggt gttctgctcg
     7381 tttcgggaga tgtagatcag gggtcccagg agatcgttcc agtagaggat gaacgtgaag
     7441 aggaagatcg aaatgagcac cggtttgatg aggggaagca ggatctgaaa cagcgtgcgg
     7501 atgtggccgg cgccgtcgat ccaggcggcc tcatccaggt cccgcgggat ggtcatcagg
     7561 aactgccggc ccaggaagat gttgaagcct ccgccgccca ggaacgccgg cacgatgagg
     7621 ggcacgaaac cgctggaaag ccccaggaag gtccagccca ggaagatcgg gatgatggtc
     7681 accgagcccg gcatgagcat ggaggcgacg accatcgtga accagaactt cttgccgcgg
     7741 aactccagcc gggcgaaagc ataggcggcg agggtggagg tgagaagcac cccgaccagg
     7801 gacggcaccg tgatggtcag cgtgttgagc aggtaccgtt ggaaagggaa ggtccgcagg
     7861 gtctccaggt agttctcgaa gtgccagacc ttcgggatcc actggggtgg gtaggtattg
     7921 atggcgatgt tggacaggaa ggaggagcgc accatccaca ggaaagggaa gacgcacgcc
     7981 agggcgagga gagagagaat cacggtcagc gccacgttgg ttcgttcggg ccgcatggag
     8041 tgcctcactt tccttcgaag aacatccacc gtcgggaggt ggcaaagatg actccggtga
     8101 gtgctccgat gaagaggaag aacaggagcg acaccgccgc ggcgtggccg aagttgttcc
     8161 gctcgaagcc ttcgcggaag atgagcagga ccatgtagag ggtggagttg ttcggtcctc
     8221 ccttggtgag cgcccgagcg gggacgaaga tctggaggtt ggtgaccagg ctcatcagca
     8281 ggttgaagaa gatgatggga gacatgagcg ggagcgtgac gtgccggaag cggtgccacg
     8341 cgtttccacc gtcgatctgc accgcctcca ggtaggagcg cgggatgccc tggagcccgg
     8401 ccaggaagat gacgatgaag ttccccaggc cccagacggt gagcgcgatg atggatgggg
     8461 tggcggtgag gtcgttgccg agccactgga ccttggcgac acccacca
//
LOCUS       DASRZS010000075         7929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_812707, whole genome shotgun sequence.
ACCESSION   DASRZS010000075 DASRZS010000000
VERSION     DASRZS010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812707"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFH83_01835"
     CDS             <1..414
                     /locus_tag="VFH83_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485127.1"
                     /translation="GLIIAYVTICLPFAAWMSKGYFDSIPKELDEAALMDGARRSTIL
                     YRVILPVTLPGLGATFVFSVLGSWNEFLFALVLTDTVDVRTLPLGIANLIGQWITSWN
                     VLMAAGVIFSIPPLILFLFLEKALVSGLTAGAVKG"
     gene            416..1438
                     /locus_tag="VFH83_01840"
     CDS             416..1438
                     /locus_tag="VFH83_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485128.1"
                     /translation="MTGRERFLAAVHGESTDSLLNIPITMMKAADAVRVPYKRYALDA
                     EAHAAGQIAIARTYDIDHVSGISDPAVEAADLGATVIYRDDAPPAIDDSHTLLADKTR
                     LATLKVPDPRSTPRMGKRIDVVARLHREVGAEKAVEGWIEGPIAQACDLRGMSAVMID
                     FFDDPPFIRDLTAFVFEMEIAFARAQVEAGADYIGVGDAAASLIGPDLYREFAWEHEK
                     RYVREIHRMGVPVRLHICGNITALLPLIREVGADLLDLDSMVSVSDARRDVGADTVLS
                     GNINPVAVVRDGSPTLVTDRLEECLADAGSRRYAVAAGCEIPRDSPDANLHAMTRFAR
                     SHRAAG"
     gene            1446..2498
                     /locus_tag="VFH83_01845"
     CDS             1446..2498
                     /locus_tag="VFH83_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485129.1"
                     /translation="MAAPAPSQPFPRLDFTFAPAWWHARFGMDFSDRRVWMDPIAYVE
                     RDREQRRLLHETFGDVDLGEADPKPRPRAGDEYGHRWMSAFWGCEVVYLVDQAPHATP
                     FPDAWERLAALRIPDVATSPVAQHTLANARVLTERYGRCECAINYGGPLNNAVSVLGE
                     SIYLACAAEPKLADRVLRQMAQAVITAHDEVACPVTGADVATARKGSWGIGNCPVGTI
                     DPQMYTRIVLPVDQWFARQFSGPFNLHHCGIFDRYREAYRPLHFDTMDLGPGSDLRAA
                     RAAYPRTPISAWVDVGVLPRLGRTGIDAMIRGIVNDLGPMELIPAIMVGEAGPEVSDE
                     TVRDFLTAPERLKLPG"
     gene            2503..3588
                     /locus_tag="VFH83_01850"
     CDS             2503..3588
                     /locus_tag="VFH83_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485130.1"
                     /translation="MAISPRQRFLQYVTNPRGALPVVSPFLPHQPVIRAALAHLGLSA
                     GDDFVANEITLARRLGYQPMFMTDFETLLFPWKEERVEGDTVVSTIEVAGARWERKVS
                     RSLGAWGSEDAFPVKEPQDHRTLALICEAVADRTDAIRAYFRAWRQRAGEEGVIVLGH
                     PHVSWLGCQIGPQTMLLHWADDPDLYRSSMEAVYRASLVIMEIALAEGIDFMSESSYG
                     LEMSSPALIEAQDLPYIRRLSTWTHRRGGKFWYHNCGHTRTLIRSGVFDRMGADLIET
                     VAPPPCGDNDLGESRRMLDREIVSKGNFDLGLLREGTVDQVTAATRAMVASVRRFPHV
                     FSTADAVLEGTPPANFIAFVETALREA"
     gene            complement(3676..6057)
                     /locus_tag="VFH83_01855"
     CDS             complement(3676..6057)
                     /locus_tag="VFH83_01855"
                     /inference="COORDINATES: protein motif:HMM:NF015589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HET6485131.1"
                     /translation="MEFNYQFDEFSESFFAGRDYCLLGNGYLRLVVQLKNNFGSSGNA
                     ILLNLLPSDDYRARRFLLLAHHAFKEMPSGVAVRQGGRFYQADSRQIGTESLRYEQTF
                     ERGIPTLRVRWQACYELSRDTTPRITDEQIEDVALAPGNIGLAAKGPSREEPRSPFTV
                     TERIWCPPGLPAVIREVTVKNTSSGVVSGMEVWHFLAPNKLFLPHARLLSDKSGNGGR
                     NETAASGYWGGARQFLGLACLDTGAWHLIAEYPNAIDMAAVRETPGRRSLRDPFMGPH
                     LAAGARLPRLGRGQSATVRFAVAYGKTQQEMTAAARALRAEKTVRVRAEKSWDALTSV
                     VTADARLDTLYRASKAGIRAAISSHEKRGRINAGILQYDAEWSRDHDFIAAAAAMNGQ
                     TDLAQDALEYALENLTDDRGHCFCGAAWAGEDKDYQIDTNGIRLWALWVHHVYTGETR
                     LLRRHAPRIRSLLGVFFDPVRWVPRAGMLASTRDCWERWENCGLVPGFELEHQVWACM
                     GLEKGADLAEILGDTALAARCRARATEIWDAVRSHPRYALMQSGHFMKRRTLAGAFQL
                     TANAYRGARVGYLEPDSSEIQPILTGLASGSSAAARATLRRMEKLWNQDGLWKHGGYT
                     RYNITSDPDQSSGPWPGVTILVARAALAARQWDVWSRAMDWVYRNAEPTWTLFEHYDY
                     VAADKKNRRWYRGGIIPWLSFAEPSILMVQDLLGFRPCRAGIAIQPILPPGVRSLKAR
                     VPYRGHRVEVVVRGRGATCRWVRVDGKPHDGFDSRGAWLEPLDKSVKVELEFG"
     gene            6180..6728
                     /locus_tag="VFH83_01860"
     CDS             6180..6728
                     /locus_tag="VFH83_01860"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6485132.1"
                     /translation="MAEADTFLLGTREGYPPRIGVLVSQLQSSRHYLLRASRDLDIAS
                     LDAPPRGLKNTLGHLLAHLDAAESLFQRITFEGRRFNEQESARYTPYFEFEGGDRSHG
                     RALESYLQDLYETRARTLDGLRQREDVWLDTPKTFMKKPSNILYYWFHYILDEARHTG
                     QIILTRKYLIPAANPDFEPYVP"
     gene            7141..>7929
                     /locus_tag="VFH83_01865"
     CDS             7141..>7929
                     /locus_tag="VFH83_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485133.1"
                     /translation="MVAPTGALYVLCLSQAHLFTLASTDQGKTFLPRPPRMGGHDLTA
                     YRQLSLLGRLLRDPITLFIAEEAGQSGIYGLSFASAAEPSLYGGYRLDDPSEGSVESY
                     RALPLGLDSFAITYLKGGSLRSTIVCGSSASPRLTPIAICDGIDAVVDYDVFERPEPG
                     HTSFWGYYLAAGHHGERILRDFTVVDGAMVESAALETAASLGNQEVQCYFLSDRSARV
                     LWRNGPRVSSFRGMGGQWQKDGETFLSGESFTLFPISDGRAFQVA"
BASE COUNT         1289 a         2697 c         2554 g         1389 t
ORIGIN      
        1 ggcctcatca tcgcctacgt gaccatctgc ctgcccttcg ccgcctggat gtcgaagggc
       61 tacttcgact ccatccccaa ggagctggac gaggccgcgt tgatggacgg ggcgcggcgg
      121 tctaccatcc tctaccgcgt catccttccc gtcacgctgc cgggcctcgg ggccaccttc
      181 gtgttctcgg tcctgggctc ctggaacgag ttcctctttg ccctggtgct cacggacacc
      241 gtggacgtaa ggacgctgcc cctggggatc gcgaacctga tcgggcagtg gatcacctcg
      301 tggaacgtgc tcatggcggc cggggtcatc ttctccatcc cgccgctgat actcttcctg
      361 ttcctggaaa aggccctggt aagcggtctg accgcgggcg ccgtcaaagg gtgacatgac
      421 cggcagagag aggttcctcg ccgcggtcca cggagaatcc acggactccc tgctcaacat
      481 ccccatcacc atgatgaaag cagccgacgc cgtccgcgtg ccgtacaagc gctacgcgct
      541 ggacgcggag gcgcacgccg cgggacagat cgccatcgcg aggacctacg acatcgacca
      601 cgtatccggg atctctgacc ctgcagtgga ggcagcggac ctgggcgcta cggtcatcta
      661 tcgggacgat gctccacccg ccatcgatga ctcccacacg cttctggcgg acaagacccg
      721 gctggcaacc ttgaaggtgc cggacccgcg gtctaccccg aggatgggaa agcgcatcga
      781 cgtggtggcc cgtctgcatc gggaggttgg cgcggagaag gccgtggaag gatggatcga
      841 gggacccatt gcccaggcct gcgaccttcg aggcatgagc gccgtcatga tcgacttctt
      901 cgacgaccct ccattcatcc gggaccttac ggcgttcgtg ttcgagatgg agatcgcctt
      961 tgcgcgggcc caggtggaag ccggcgcgga ctacatcggc gtgggggacg ctgccgcgtc
     1021 cctgatcggt ccggatctgt accgcgagtt cgcctgggag cacgagaagc gctacgtgcg
     1081 ggagatccac cgcatgggtg tcccggtgcg gcttcacatc tgcgggaata tcacggcgct
     1141 ccttcccctg atccgcgagg tgggcgccga cctcctggac ctggactcca tggtctccgt
     1201 ctccgatgcc cgacgggacg tcggggccga caccgtcctc tccggcaaca tcaaccccgt
     1261 tgccgtggtt cgggacggga gcccaacgct ggtcacggac aggctcgagg agtgccttgc
     1321 tgacgcagga agcaggcggt atgccgtggc agccggctgc gagattccac gcgacagccc
     1381 ggatgccaac ctgcacgcca tgacccgctt tgcgcgatcc catcgcgcag caggctgaac
     1441 gcgccatggc agccccggcg ccgtcccagc cgttccccag gctcgacttc accttcgccc
     1501 ccgcatggtg gcatgcgcgg ttcggcatgg acttctccga ccggcgtgtg tggatggacc
     1561 ccatcgccta cgtcgagcgc gaccgcgagc agcggcgcct gctccacgag accttcggtg
     1621 acgtggacct gggggaggcg gatccaaagc cccggcctcg cgcgggtgac gagtacggcc
     1681 accgctggat gtccgcgttc tggggatgtg aggttgtcta cctggtggac caggcgcccc
     1741 atgccacccc atttcccgac gcgtgggaac ggctggccgc gctgagaatc ccggacgttg
     1801 ccacctcccc ggtggcccaa cacaccctgg ccaacgctcg cgtcctgacg gagcgctacg
     1861 gccggtgtga gtgcgccatc aactatggcg gccccttgaa caacgccgtc tcggtcctgg
     1921 gagagtccat ctacctcgcg tgcgccgccg agccgaagct ggcagatcgg gtgctccgtc
     1981 agatggcgca agcggtcatc acggcccacg acgaggttgc ctgccccgtc accggggcgg
     2041 acgtggccac ggcgcgcaag gggtcctggg gcatcggcaa ctgcccggtg gggaccatcg
     2101 atccccagat gtacacgcgc atcgttcttc ccgtggatca gtggtttgcc cgtcagttct
     2161 ccggcccctt caacctgcat cattgcggga tcttcgatcg ctatcgagag gcctatcggc
     2221 cgctgcattt cgacaccatg gaccttggcc ccggctccga ccttcgcgcg gcgcgggccg
     2281 cctacccgcg gacgcccatc tccgcctggg tggacgtggg ggttctgccg cgcctgggac
     2341 gcaccggcat cgacgcaatg atccgcggca tcgtcaatga cctgggaccc atggagctga
     2401 tcccggccat catggttggc gaggcgggac ccgaggtctc ggatgagacg gtccgggatt
     2461 tcctcaccgc tcccgagcgt ttgaagcttc ccgggtgacg ggatggcgat ctcaccacgg
     2521 cagcgctttc tccagtacgt gacaaaccca cggggcgccc tccccgtggt gtccccgttc
     2581 ctcccgcacc aaccggtgat ccgcgcggcc ctggctcacc tggggctttc ggccggggat
     2641 gacttcgtgg ccaacgaaat caccctggcg cggcgtctcg gctaccagcc catgttcatg
     2701 acggacttcg agacgctgct cttcccctgg aaggaagagc gcgtagaggg cgataccgtg
     2761 gtctccacga tcgaagtggc gggagcccga tgggagcgca aggtctcgcg ctctctgggc
     2821 gcgtggggca gtgaggacgc gttccccgtg aaggaaccgc aagaccatcg cacgctggca
     2881 ctcatctgtg aggccgtggc ggaccgcacc gatgccatcc gcgcatactt tcgcgcctgg
     2941 cgccagcggg cgggagagga aggggtgatc gtcctgggtc acccgcatgt ctcgtggctg
     3001 ggctgtcaga tcgggcccca gaccatgctc ctgcactggg ccgatgatcc cgacctctac
     3061 cgatccagca tggaggcggt ctaccgcgcc tccctggtca tcatggagat cgcccttgcc
     3121 gaggggatcg acttcatgag cgagtccagc tacggcctgg agatgagctc tccggccctg
     3181 atcgaggcgc aggacctgcc ctacattcga cggctttcga cgtggaccca ccggcgcggc
     3241 ggcaagttct ggtatcacaa ctgcggccac acccggacgc tgatccgatc cggcgtgttc
     3301 gaccgcatgg gagcggacct catagagacc gtggccccgc ccccctgtgg ggacaacgat
     3361 ctgggcgagt cgcggcggat gctggaccgt gagatcgtga gcaagggcaa cttcgacctt
     3421 ggcctgctgc gggaagggac cgtggatcag gtcacggccg caacgcgcgc catggtcgct
     3481 tcggtgcgcc ggttcccgca cgtcttctcc accgcggatg ccgtgctgga gggaacgcct
     3541 ccggcaaact tcatcgcgtt cgtcgagacg gcactgcgag aggcgtaggg gccgcggcgc
     3601 ttcaggaagc gtgggggccg cagcgcttgc acccaggagc gcgggacgct tcgggtagcc
     3661 cgagggcctc gctgctcagc cgaactccag ctccaccttc accgacttgt ccagtggctc
     3721 gagccacgcc ccgcgggagt cgaacccgtc gtgaggcttg ccgtcgactc ggacccagcg
     3781 acaggtggcg ccccgccctc tcaccaccac ctccaccctg tgtccccggt acggcacgcg
     3841 cgccttcagg gagcgcaccc ccggcgggag gatgggctga atggcgatac cggcacgaca
     3901 cggccggaaa ccgaggagat cctggaccat caggatggat ggctccgcga aggagagcca
     3961 tgggatgatg ccgcctcgat accagcggcg gttcttcttg tccgcggcca cgtagtcgta
     4021 gtgctcgaag agcgtccacg tgggttccgc gtttcggtag acccaatcca tggcacgact
     4081 ccacacgtcc cactgccgcg ccgccagcgc cgcgcgcgcc accaggatgg tgacgcccgg
     4141 ccacgggccc gagctctggt cggggtccga ggtgatgttg taccgggtgt atccgccgtg
     4201 cttccaaagc ccgtcctggt tccagagctt ctccatccgc cgcagggtgg cgcgcgcggc
     4261 ggccgagctc cccgaggcaa ggccggtcag gatcggctgg atctcgctgg agtctggctc
     4321 caggtatccc acccgggccc cgcggtacgc atttgccgtg agctggaacg ctcccgccag
     4381 cgtccttcgc ttcatgaagt gaccggactg catcagcgcg taccgcggat gggagcgcac
     4441 cgcgtcccag atctccgtgg ctcgagcgcg gcatcgcgcg gccagcgcgg tatcgcccag
     4501 gatctccgcc aggtccgcgc ccttctccag acccatgcac gcccacacct ggtgctccag
     4561 ctcgaagccg ggcaccagcc cgcagttctc ccaccgctcc cagcagtccc gggtgctggc
     4621 caacatgccg gcccgcggga cccaacgcac cgggtcgaag aagactccca gcagagaccg
     4681 tatccttgga gcgtggcgtc ggaggagccg ggtctctccc gtgtagacat ggtgcaccca
     4741 gagggcccag aggcggatcc cgttggtgtc gatctggtag tccttgtcct ccccggccca
     4801 cgccgcgccg cagaagcagt ggcctcgatc gtcggtgagg ttctccagcg cgtactccag
     4861 cgcatcctgg gccaggtccg tctgcccgtt catggcggca gccgcggcga tgaagtcgtg
     4921 gtcccggctc cactccgcgt cgtactgaag gatgcccgcg ttgatccttc cccgtttctc
     4981 gtggctgctg atagccgcgc ggatgcccgc cttggaggcg cggtaaagcg tgtccagccg
     5041 cgcgtctgcc gtgaccacgg aggtcagcgc atcccacgac ttctcggctc gtacccgaac
     5101 cgtcttctcc gcccgcagcg cacgcgccgc tgccgtcatc tcctgctgtg tcttgccgta
     5161 ggcaacggcg aacctcacgg tggcggactg accccggccc agtcgaggga gccgggctcc
     5221 ggcggccagg tgcggtccca tgaacgggtc ccgaagggaa cgccgtcccg gcgtttcccg
     5281 tacggctgcc atgtcgatgg cgttcgggta ctcggcgatg agatgccacg cccccgtgtc
     5341 caggcaagcg aggcccagga attgccgcgc gcctccccag tagccactcg cggcagtctc
     5401 attccgcccc ccgttgcccg acttgtccga gaggagccgc gcgtgtggga ggaacagctt
     5461 gttgggcgcc aggaagtgcc agacctccat cccggaaacg acgccagagc tcgtgttctt
     5521 gacggtgacc tcccggatca cggcgggcag acccggcgga caccagatcc gctcggtaac
     5581 cgtgaagggg gagcgaggct cctcgcgtga aggccccttc gccgcgaggc ctatgtttcc
     5641 gggcgccaga gccacgtcct cgatctgttc gtcggtgatc cgcggcgtgg tatcccgaga
     5701 aagctcgtaa cacgcctgcc atcggacccg gagggtcggg atcccgcgtt cgaaggtctg
     5761 ctcgtaccgt agcgactcgg tgccgatctg gcgtgaatct gcctggtaga accggccccc
     5821 ctgtcggaca gccacgccgg agggcatttc cttgaacgcg tggtgcgcca ggagcaggaa
     5881 acgtcgggca cggtagtcat ctgagggaag caggttcagg agaatggcgt ttccgctgga
     5941 gccgaagttg ttcttgagct ggaccaccag gcgcaggtag ccgtttccca ggaggcagta
     6001 gtcgcgcccg gcaaagaagc tctcgctgaa ctcgtcgaac tggtagttga actccatcga
     6061 gccccgggaa caaggctatc gtcggagccg ggggcggtgt caagggaagg ggcctgtggt
     6121 agaaggggtg cggacgcgcc gcctggaccg ttggaccgcc ccgtctctat actgctggca
     6181 tggccgaagc cgacacgttt cttctgggaa cccgcgaagg ctacccgcct cgcatcgggg
     6241 tgctggtcag ccagctccag agctcacgcc actaccttct gcgcgcctcc cgcgatctgg
     6301 atatcgcgag cctggacgcg ccgccgcggg ggctcaagaa cacgctcggc caccttctgg
     6361 cccacctgga cgccgcggag agcctgttcc agcgcatcac cttcgagggc cggcgcttca
     6421 acgagcagga gtcagctcga tacacgccgt acttcgagtt cgaggggggc gatcgatccc
     6481 atggtcgcgc tctggaatcg tatctccagg acctgtacga gacccgggcc cgtacgctgg
     6541 acggtctgcg tcagcgggag gacgtctggc tcgacacccc gaagaccttc atgaaaaagc
     6601 cgtcgaacat cctctactac tggttccact acatcctgga cgaggcccgg cacaccggcc
     6661 agatcatcct gacccgcaag tacctgatcc cggccgccaa tcccgacttc gagccgtacg
     6721 tgccgtgaag agggctcgcc ccgcgccaga ggtatcaccc gaaatggtgc ttgcatttgc
     6781 atcggcccca tgctacaaca gcggcgcatc aaaaagtttc gatgacaaaa aaggaagagg
     6841 gtcgctatga gtgctgcgca ttgttcgacg aaccggtggc ctgctgatcg tagacgcctg
     6901 gacctctctg aaactcagac tcagctcttg aacctgtcct gacgggatgc gcgcgtgtcc
     6961 tacgcatccc cggcgccccg cggcccgcgg gaacgacccc gcgcgctcct ggtctcggcg
     7021 ttcgcgctga cctgtgcgat tctggcgtct gccccggccc ctgccttggc cgatgaggct
     7081 tttcgagcgc tgaatggcgg cgcgcccctc acgcaggggc cggtcacgga agctgtttcg
     7141 atggttgcgc ccaccggcgc gctgtatgtc ctctgcctgt cccaggcgca cctgttcacc
     7201 ctggcatcca ccgaccaagg aaagacgttt ctgccgcggc cgccgcgcat gggcggccac
     7261 gacctgaccg cctatcgaca gctatccctc ctgggacgtc tgctccgaga ccccattacg
     7321 ctcttcatcg cagaagaggc gggccagtca gggatctatg ggctctcctt cgcttccgcc
     7381 gcggagccgt cgttgtatgg tggatatcgg ctggacgatc cgtccgaggg aagcgttgag
     7441 agctaccgcg cgcttccgct ggggttggac agctttgcca tcacctatct gaaagggggc
     7501 tcgctccgga gcacgattgt ctgcggttct tctgctagcc cgcggctcac gcccatcgcg
     7561 atatgcgatg ggatcgacgc agtcgttgac tacgacgttt tcgagcgacc cgagcccggc
     7621 catacttcct tctggggata ctacctggcc gccggccatc acggagaacg gatccttcgc
     7681 gacttcacgg tggttgatgg cgcgatggtg gaaagcgctg cgctggagac cgccgcgagt
     7741 ctggggaacc aagaggtcca atgctacttc ctgtcagacc gttctgcccg cgtcctctgg
     7801 cggaacggcc cacgcgtcag cagttttcga gggatgggag gccaatggca aaaggacgga
     7861 gagactttct tgtccgggga gagcttcacc ctctttccga tcagtgatgg cagagcattt
     7921 caggtagca
//
LOCUS       DASRZS010000076         2642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1968169, whole genome shotgun sequence.
ACCESSION   DASRZS010000076 DASRZS010000000
VERSION     DASRZS010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968169"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VFH83_01870"
     CDS             complement(<1..747)
                     /locus_tag="VFH83_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HET6485134.1"
                     /translation="MESDIKAPDPWKKENKDEGGVHHPQPFFSFNNPQFRFSFWYILV
                     GIVVFFLVNALVSQSFGPQEQTISFSEFKQKIASGEIKQVQMDDSSYLGLTVTKAQEA
                     SSSQQQNQPLTVYKTVRVEDPQFIALLDSNHVEYYAVEKREHPFLNLLLSTVLPIVVM
                     VVIWRFAFKRMGNAGSGVLSFGQNKAKVVAEGDIKVRFGDVAWVDEAKEELVEVVDFL
                     KRPERYTAIGGKIPKGVLLVGSPGTGKTLLA"
     gene            1034..1717
                     /locus_tag="VFH83_01875"
     CDS             1034..1717
                     /locus_tag="VFH83_01875"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET6485135.1"
                     /translation="MIPLGDASRRPRRFPVVTLSIIAVNVFVFILELLGGDDFILRWS
                     LEPAGILTVHGVITVFTSMFMHAGWEHIIGNMLFFWVFAPDIEDAMGRFTFLLFYLLG
                     GVVAAVAEVVVNPSATIPSLGASGAIAAVMGAFLITYPRDRIRTVLFFGFFVRVAYVP
                     AVLMIGLWILTQVFSQVGAMAQVQTGGGVAYMAHIGGFIYGVLTGRPFETHQQRRLQG
                     PDRTERLPW"
     gene            1711..>2642
                     /locus_tag="VFH83_01880"
     CDS             1711..>2642
                     /locus_tag="VFH83_01880"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET6485136.1"
                     /translation="MVMNADRTLHRESRGKRLRARYREIAAVLWDERLLELLRGSGLQ
                     EHLSEREPADKAPGARREGQPVEVRLRHALERLGPVFTKIGQHLATRGDLLPPALLRE
                     LARLQDDVPAEPWPRMKACIEAELGAPLKRLYRSITHTPLAAASLGQVHRATLSDGTP
                     VAVKIQRPGITEAVEIDLEVTHDLASRLAKRVRWARDADLVAIVEDFAAALRAELDYA
                     REGRSLDRFRTAFADDPSLVIPRVYWDRTTSRVLTMDFIDGVPGTQMENSEKTAGIDR
                     AALVRSGVGAYFRMIFQLGFYHADPHPGNLIALPG"
BASE COUNT          451 a          844 c          837 g          510 t
ORIGIN      
        1 ggccaaaagg gtcttgcctg tccccgggga tcccaccagc agcaccccct tgggaatctt
       61 gcccccaatc gccgtgtagc gctcgggccg cttcagaaag tccaccacct ccacaagctc
      121 ttccttggcc tcgtccaccc aggccacatc cccaaagcgc accttgatgt ccccctccgc
      181 caccaccttc gccttgttct gcccgaagct cagcacccca gaacccgcgt tccccatgcg
      241 cttgaacgcg aagcgccaga tgacgaccat gaccacgatg ggcagcaccg tgctgagcag
      301 caggttcaga aacgggtgct cgcgcttctc caccgcgtag tactccacgt ggttgctgtc
      361 caacagggcg atgaactgcg ggtcttccac gcggacggtc ttgtacaccg tcagcggttg
      421 gttctgttgc tgcgagctgg atgcttcctg cgccttggtc accgtcagac cgaggtagga
      481 gctgtcgtcc atctgcacct gcttgatctc cccactggcg atcttctgct tgaactcgct
      541 gaagctgatc gtttgctcct gcgggccgaa ggactgggag accagggcgt tgaccaggaa
      601 aaagacgacg atgccgacca ggatgtacca gaacgagaaa cggaactgtg gattgttgaa
      661 ggagaagaac ggttgcgggt gatgtacccc tccctcgtcc ttgttctcct tcttccaggg
      721 gtccggggcc ttgatatcag attccataga ttcctccttg cttagtcagt tgacttcacc
      781 cccaaaccat atgctcggga cggcatggct gtaaccggcc gaaggtacgg taagggtacg
      841 gtggccgaac gcggtcacgg gctgcgccac gtcagcggcg gtggcatacg gaccgagcgc
      901 ggctagccca cgactgtacc tttggccgat gccaaccggt cgccggccgc tctatcgttg
      961 agccgttcgg cacgaaaaga tatcggaagt cacgaggcac cgattctttc gaagagtgcc
     1021 gggaggtgaa ggaatgattc cacttggaga cgcgtcacgg cggccgcgga ggttccccgt
     1081 cgtaaccctg tcgatcatcg cggtgaatgt cttcgtgttc atcctggagc tgttgggagg
     1141 cgatgatttc atcctgcgct ggtcgctcga gccagccggt attctcacgg tccatggggt
     1201 gatcaccgtc ttcacctcga tgttcatgca tgccggctgg gagcacatca tcggcaacat
     1261 gcttttcttc tgggttttcg ccccggatat cgaggatgcg atggggcgat tcacctttct
     1321 gctcttctat ctcctgggag gagtggtggc cgctgtagcc gaggtggtgg tcaacccctc
     1381 ggctacgatc cccagtctcg gagccagcgg cgcgattgcg gccgtgatgg gcgcgttcct
     1441 gatcacctac ccgagggaca ggatccgtac ggtgctcttc ttcggtttct tcgtgcgggt
     1501 ggcctacgtg cccgccgtcc tgatgatagg gttgtggatt ctcacccagg tcttcagcca
     1561 ggtgggagcg atggcgcagg tccagaccgg aggcggcgtg gcgtatatgg cgcacattgg
     1621 cgggttcatc tacggcgtgt tgacgggccg gccgttcgag acccaccagc agcgtcgttt
     1681 gcagggtccg gacaggacgg aacgcctgcc atggtgatga acgctgaccg aacgctgcat
     1741 cgggagagtc gcggcaagcg gctccgggcg aggtaccggg agatcgccgc ggtgctgtgg
     1801 gatgagcgcc tgttggagct cctccgcggc agcgggctcc aggagcatct gtccgagaga
     1861 gagccggctg acaaggcccc cggtgcccgc cgagaaggcc agcccgtcga ggttcgcctc
     1921 cgccacgcgc tggagcgcct cgggcccgtc ttcacgaaga tcgggcagca cctggccacg
     1981 cgcggcgatc tgcttccgcc cgctctcctt cgagagcttg ccaggctcca ggatgacgtg
     2041 ccggcggagc cttggccgcg gatgaaagcg tgcatcgaag cggagctggg cgcgccgctc
     2101 aagaggctgt atcgaagcat cacccatacg cccctggcgg ccgcctcgct gggccaggtc
     2161 catcgcgcga cgctgtccga cggcactccg gtagccgtga agatccagcg acccggcatc
     2221 acggaagcgg tggagatcga cctcgaggtc acgcacgacc ttgccagccg gcttgccaag
     2281 cgcgtgcggt gggcaagaga cgccgacctg gtcgccatcg tcgaggactt cgcggccgcc
     2341 ctgcgggcgg agctcgacta cgcccgtgag gggcgttccc tggaccggtt ccgcaccgcg
     2401 ttcgccgacg acccctcgct cgtcattccc cgggtctact gggaccggac gacgagccgc
     2461 gtgctcacga tggacttcat cgatggggtg cccgggacac agatggagaa cagcgaaaaa
     2521 accgctggta tcgaccgggc cgccctggtg cggagcggcg ttggagccta cttccgcatg
     2581 atcttccagc tcgggttcta ccatgcggat cctcatcccg gaaacctgat cgcgctgccc
     2641 gg
//
LOCUS       DASRZS010000077         4183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1968405, whole genome shotgun sequence.
ACCESSION   DASRZS010000077 DASRZS010000000
VERSION     DASRZS010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968405"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VFH83_01885"
     CDS             complement(<1..547)
                     /locus_tag="VFH83_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485137.1"
                     /translation="MTDDLSREAQARIEKMVERVARKAGYSGRGTMTYRELLQVKLEQ
                     KRRKLQGKVDRYRRKLSLKPSNTDFAEEIRAYLADGVIDLVKEGRSEDEAMEITLKKF
                     DDAELRGSFDDFAKALGGFGMEEWMKESIKEWTVTNGDAIGLFYAAFTVLGMTLGTLL
                     GYLLGRAWQTTAIGLAAGLLFG"
     gene            complement(537..884)
                     /locus_tag="VFH83_01890"
     CDS             complement(537..884)
                     /locus_tag="VFH83_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016113594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET6485138.1"
                     /translation="MEIEKEMLKGHIDLIILALLNEEDFYGYELGKRVKAQTQEGFEL
                     KEGTLYLAFKRLEEGGLVQSYWGDEAAGGGRRKYYRLLEKGRQRLRAGKKEWEHLKKI
                     VDQFLEEVDANDG"
     gene            1167..1487
                     /locus_tag="VFH83_01895"
     CDS             1167..1487
                     /locus_tag="VFH83_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016123014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET6485139.1"
                     /translation="MNIQFKKGVLELCVLVVLCRKDYYGYELVQAISKNIDIAEGTVY
                     PLLRRLTKEGYFKTYIQESATGPARKYYQITEKGKEYQKVLLEDWNRFISGVNNLIGK
                     EEVA"
     gene            1484..2068
                     /locus_tag="VFH83_01900"
     CDS             1484..2068
                     /locus_tag="VFH83_01900"
                     /inference="COORDINATES: protein motif:HMM:NF019618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HET6485140.1"
                     /translation="MNKETFLSELKRSLGRMPESEKKDVLYDYEEHFRMGTAEGKSED
                     QVAASLGNPRLIGKSYAIDSLLEDRKEGGQAPASFVLRAVFASISLTFFNLVVVLGPF
                     VGLVGVMIGLWATAISLPISGVAAVLAPLAALIIPQWVSLGGLSAFFWIFGGIGVAAL
                     GVLAVIGMYLLSRLFLRATAAYVKLNARIVTRRK"
     gene            2078..3010
                     /locus_tag="VFH83_01905"
     CDS             2078..3010
                     /locus_tag="VFH83_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET6485141.1"
                     /translation="MATNTNLKRVVIVTAAVAVAALGIAGLVALASGGFGAVGAFGRA
                     GNQVDQRKSAALAGVEQIRIEGVSDDVTVKTVDGDSLDAWFHGSVGGSDPANAPKLTV
                     EQDGSAVLVRIERGPVRFGFFWSDLKLDVSVPAAYTGSVSVNVVSAKVDLQDHQYSGV
                     TVSTVSGDVLVGAVKSQAFSARTTSGRVQASSVESSTAELSTVSGEIEVRSLSGATAV
                     HTTSGSVSITYASMPAAVDASSTSGDVLLRFPQDAAFGLDARSTSGDVKCGFPITITE
                     SASGGGRHALAGTVGNGGNKVSVRTVSGSIQVTR"
     gene            3231..>4183
                     /locus_tag="VFH83_01910"
     CDS             3231..>4183
                     /locus_tag="VFH83_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET6485142.1"
                     /translation="MDELGFLTTLVGGLGGALILGYLAQRLKLSPIIGYLLAGIIVGP
                     FTPGFVANHEIAQQFAEIGVILLLFGIGLRFHLREMLAVWRIAVPGAIFQSALSTVAL
                     ALLLTLLGWSWTSGIILGLSISVASTVVMSLVLGARGDLHARIGHIAMGWTVVEDLLT
                     VVMLLLLPIVFGPATEAHPGALASLGIAGLNVVGLVCVVVVLGRWAIPWALGQIAKTR
                     SRELFTLAVLVLALGIAVGSARLFGVSMALGAFLAGMIVGRSDYALRAASEALPMRDA
                     FAVLFFVSVGMRFDPHSLADAPGLVAGMLAVIFLGKPVAALG"
BASE COUNT          674 a         1324 c         1359 g          826 t
ORIGIN      
        1 tgccgaagag caaccccgca gccagcccaa tcgcggtagt ctgccatgcc cgaccaagga
       61 ggtagcccag gagcgtcccc agggtcatcc cgagaaccgt gaacgcggcg taaaacaatc
      121 cgatggcgtc gccgttcgtc acggtccatt ctttgatcga ttccttcatc cattcctcca
      181 tgccgaaacc tcccaacgcc ttcgcaaagt catcaaagct gcccctcagc tccgcatcgt
      241 cgaacttctt cagagtgatc tccatggcct catcttccga acggccctcc ttcacgagat
      301 cgatgacgcc atcggccagg tatgcgcgga tttcctccgc gaagtcggtg ttgctcggtt
      361 tcaggctgag cttgcgtcgg tatcgatcga ccttcccctg cagcttgcga cgcttctgtt
      421 cgagcttgac ctggaggagc tcccggtagg tcatggttcc ccggccggaa taacccgcct
      481 ttcgggccac ccgctccacc atcttctcga tcctggcctg cgcctctcgg ctcagatcat
      541 ccgtcattgg cgtccacctc ctccaggaat tggtccacga tcttcttcag gtgctcccat
      601 tctttttttc cggcgcgtag acgctgccgg cctttttcca ggagcctgta atacttgcgc
      661 cgccctccgc cggctgcctc gtcgccccag tacgactgca ccagcccgcc ctcttcgagc
      721 cgcttgaagg cgaggtagag ggtcccctcc ttcagctcga acccttcctg ggtctgggcc
      781 ttcacgcgct tacccagttc gtagccgtag aagtcctctt cgttcagcaa cgcaaggatg
      841 atgaggtcga tgtggccttt cagcatttcc ttttcgattt ccatggcctc caccaggcgg
      901 ctttcgccgg ccgctaccca cactggtcaa aatacattgt aacataaact aatctatatt
      961 actatgtact ttgctaccct tggatctgat ccggctcact gaagcgaaac ggatgtttag
     1021 cttttcctcg ctgccgggag gatcggcctc agaaaaacag cgagaacggg tattgcattg
     1081 atctgtactg tgcattaaat actagtagct aagaaatggt agttggcata agccactata
     1141 aggcataggc cactacagga ggaaccatga acattcaatt caagaagggg gtgctggagc
     1201 tctgcgtgct ggtggtgctg tgccgcaagg actactacgg ttacgagctg gtccaggcga
     1261 tctcgaagaa catcgacatc gccgaaggca cggtgtaccc gcttctgcgt cggctgacca
     1321 aggaagggta tttcaagacc tacatccagg agtccgccac ggggcccgcg cgcaagtact
     1381 accagatcac cgagaagggc aaggagtacc agaaggtgct gctggaggat tggaaccgct
     1441 tcatcagcgg cgtgaacaac ctcatcggga aggaggaagt ggcatgaaca aggaaacctt
     1501 tctgtcggag ctgaagcgtt ccctgggccg gatgccggag agcgaaaaga aggacgtgct
     1561 ctacgactac gaggagcact tccggatggg gaccgcggaa ggcaagagtg aggatcaggt
     1621 agccgcctct ctgggcaacc cacggctgat cgggaagtcc tacgccatcg actccctcct
     1681 ggaggaccgc aaggagggcg gccaggcgcc ggccagcttc gtgttgcggg cggtcttcgc
     1741 ctccatcagc ttgaccttct tcaacctcgt tgtcgtcctg ggccccttcg tgggcctggt
     1801 gggagtgatg atcggcttgt gggccacggc gatctccctg ccgatctcgg gcgtcgcggc
     1861 ggtgctcgcg ccgctggcgg ccctgatcat cccgcagtgg gtgagcctgg gaggcctgag
     1921 cgcgttcttc tggatctttg ggggaatcgg ggtggcggcg ctgggggtcc tggccgtgat
     1981 cggcatgtac ctgctgagcc ggctgttcct gagggcaacc gccgcatacg tgaagctcaa
     2041 cgcgcgcatc gtgacgcgca ggaaatagga ggacatcatg gcaacgaata cgaatctcaa
     2101 gcgcgtcgtg atcgtgaccg cggctgtggc agtggcggcc ctgggcatcg cgggcctcgt
     2161 ggctctggcc tcgggaggat tcggcgccgt aggggccttt ggccgggccg gcaaccaggt
     2221 ggatcagcgc aagagcgcgg cgctggcggg cgtggagcag ataaggatcg agggggtcag
     2281 cgacgacgtc accgtcaaga ccgtggacgg cgattccctg gacgcctggt tccacggctc
     2341 ggtgggcggg agcgatccgg ccaacgcccc caagctgacc gtcgaacagg acggatccgc
     2401 ggtcctggtg cgcatcgagc ggggccccgt gcgcttcggg ttcttctggt ccgacctcaa
     2461 gctggacgtc agcgtgccgg ccgcctacac ggggagcgtc tccgtgaacg tggtctcggc
     2521 gaaggtcgac ctgcaggacc accagtactc gggcgtgacg gtgtccacgg tctcgggcga
     2581 tgtgctggtc ggcgccgtga agtcgcaggc cttcagcgct cggaccacct cggggcgcgt
     2641 gcaagcctcg agcgtcgagt cgtcaacggc ggagctgtcc accgtcagcg gcgagatcga
     2701 ggtccgttcc ttgagcggcg ccacggcggt gcataccacc tccggaagcg tgtccatcac
     2761 ctatgcgtcc atgccggccg cggtggatgc cagcagcaca tcgggtgacg tgctcctgcg
     2821 cttcccgcag gacgccgcct tcggtctgga tgcgcggtcc acctctggag acgtgaagtg
     2881 cgggttcccg atcaccatca ccgagtccgc ctccggcggc gggcgccatg cgctggccgg
     2941 cacggtcggc aacggcggca acaaggtctc cgtccgaacg gtttcgggca gcatccaggt
     3001 gaccaggtag tccagtctcc gtggcggagg ggcggagggg cggaggtcag gcgggccgaa
     3061 aggcccgctt cctcatgccc gcgggaaccg cccgcggcag cgaagcgtcc gccccgcgga
     3121 atcgacccgg gagcgctttt cggcggtgga tcgtagcacg cggccggagg ttgcccggtc
     3181 gccggaccgg gcgtagattc gcggcatgga gcacgcgcga ggagccctgg atggatgagc
     3241 tcggctttct gacgacgctt gtcggcggcc tgggcggcgc gctgatcctc ggctacctgg
     3301 ctcaacggct gaaactgtcc cccatcatcg gttacctcct ggcgggcatc atcgtcggcc
     3361 cgttcacccc gggattcgtc gccaaccacg agattgcgca gcagttcgcg gagatcggcg
     3421 tgatcctgct gctcttcggg atcggcctgc gcttccacct tcgggagatg ctcgccgtgt
     3481 ggcggatcgc cgtccccggc gcgatcttcc agagcgccct ctccaccgtt gcgctcgcgc
     3541 ttctcctcac gctcctggga tggagctgga cgtcggggat catcctgggt ctttcgatct
     3601 cggtggccag caccgtcgtg atgtccctgg tgctcggcgc tcgcggggac ctgcacgccc
     3661 ggatcggaca catagcgatg ggatggacgg tggtggagga cctgctcacg gtggtcatgc
     3721 ttctcctgct tcccatcgtg ttcgggccgg ccacggaagc gcatcccggc gcgctggcat
     3781 ccctggggat cgccggtctc aacgtggttg ggctggtctg cgtcgtggtg gtcctggggc
     3841 gctgggccat cccctgggcc ttgggacaga tcgcgaagac gcgctcccgg gagctgttca
     3901 ccctagcggt gctcgtgctg gccctgggca ttgcggtggg ctctgcccgt ctgttcggcg
     3961 tgtccatggc gcttggcgcg ttcctggccg gcatgatcgt cggccgatcg gactacgccc
     4021 tgcgcgcggc atccgaggcg ctgccgatgc gcgatgcgtt cgccgtgctg ttcttcgtct
     4081 cggtcggcat gcggttcgac ccgcactcgc tggccgacgc gccggggctg gtcgcgggca
     4141 tgctggcggt gatcttcctg ggcaaaccgg tggcggcgct ggg
//
LOCUS       DASRZS010000078         4778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4276519, whole genome shotgun sequence.
ACCESSION   DASRZS010000078 DASRZS010000000
VERSION     DASRZS010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4276519"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /gene="prmC"
                     /locus_tag="VFH83_01915"
     CDS             complement(<1..822)
                     /gene="prmC"
                     /locus_tag="VFH83_01915"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET6485143.1"
                     /translation="MTARSLLKQGYDKLFYAEVETPLLDAVVLLAHASALTKEKLLAS
                     LPDPVGEGIEDAFQALIDRRCAGTPVSYLRRQKEFYGLDFYVDERVLVPRPDTEVLVE
                     KALRVARGDPRLRRVHDSCAGSGCVGISLQNAMPQLVVSASDLSPQALEVLEMNARRL
                     LSNPFPAYHSDLLERVPGRFDLIVSNPPYLRDDEMEDMRKLSWPEPELALRGGTDGTS
                     VAERLIRSAPERLAPGGWLLLEAAPLQITKLYALMDQAGFHSIDVDQDLGGRDRVI"
     gene            complement(819..1889)
                     /gene="prfA"
                     /locus_tag="VFH83_01920"
     CDS             complement(819..1889)
                     /gene="prfA"
                     /locus_tag="VFH83_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET6485144.1"
                     /translation="MFEKLDKARARHQELEGQLADPEVVKDQKRFREMRQEHSALSEL
                     VAEYDRYRGLAAEISSNTELAETSEDRELREMAKEELRALEERRLASEQRLKSLLVPP
                     DPLDDKNVIVEIRAGTGGEEAALFAADLFRMYSYYSDRQKWKVEIMSSSPTGIGGFKE
                     IISAISGKGVYARLRYESGVHRVQRVPVTEAGGRIHTSTASVAVLPEVEETEIEINPN
                     DLRIDVMRAGGPGGQSVNTTDSAVRITHLPTGIVVHCQDEKSQHKNKAKALRILRARL
                     YEAEEERKHAERAQARKSQIGSGDRAEKIRTYNFPQNRATDHRINLTLYKLDAVVKGE
                     IDEFVEALRLSSQESALAAESP"
     gene            complement(1946..3280)
                     /locus_tag="VFH83_01925"
     CDS             complement(1946..3280)
                     /locus_tag="VFH83_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET6485145.1"
                     /translation="MSVFKTYDIRGVWEQGIDLPFAYRLGRTLLRYRKAKGWLLGYDA
                     RAHSRELYQALASGLLEEGAQVTGVGLCSTPMLHYSQMDGKYEAAVMVTASHNPKEYH
                     GFKVFDATGGSLSYDKGLKDVEALFDKVPEVRTVPTRSFPEKDTLEAYIRFIAGSGQG
                     KVPAGPVVIDASNGSGGKEFRALANALGVDARLLNETPDGTFPNHDPNPLKEESRRQA
                     AEAVVQAGAILGVILDGDGDRILFVDEKGRGIENYFLSCLVAEGILRRQAGTSIVYDL
                     ISSRVLPERVTEMGGRPVVSRVGYTFLYDQMVASRAAFGAETSGHVYFRVSDTYYTES
                     AAYALASLLRFLAQEGRPLSALVDPLRSRYVQSPEINVEVKDKERAMREIERRYAGGK
                     MDRLDGISVEFPDYWFNVRPSNTEPLLRLRLEGRGAAVVDARTKELRALLEQ"
     gene            3500..>4778
                     /locus_tag="VFH83_01930"
     CDS             3500..>4778
                     /locus_tag="VFH83_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6485146.1"
                     /translation="MKYSSLAALKSRGTDGNYQILTYAELYTAVSELGTGLISVGLAA
                     GMHVALVAENGPRWLISDLAILGIGGVDVPVSTGVADTELEQVVAHGDCEMAVVDNAG
                     TLSRLLAMRKRIPRVRRILVLDFTGPKPSAGTGEERVLVYTWEEMIQKGRTRIARGQR
                     TFDLRAASVTPADPATLLYTSGTTGKPKGVMLSHGNFMHNVTTIHASISPRPGSAWLS
                     VLPVWHSFERTVEYCSIYFGSTIAYSQAAEWRLLEDLGTLGPNYLVLMPALLETLAKS
                     IEKRLSFVENLLVRFEKFYLVFSGFIMGRYPRFRREERLLEIFAAVPALLTLSPIKLV
                     SALTLRRKIRSLLGGALRAIVYGGGPLPAYLDRFFAAIGVDVLEGYGLTEASPIVSVR
                     SERAPVLGTAGRPLPSTEVRIVGDKGEVLPPGRK"
BASE COUNT          774 a         1631 c         1542 g          831 t
ORIGIN      
        1 gatgacccgg tcccggccgc ccaaatcctg gtccacgtcg atgctatgga accccgcctg
       61 gtccatgagc gcatagagct tggtgatctg cagaggagcc gcctccagca ggagccaccc
      121 tccgggggcc agccgctccg gcgccgagcg gatcagacgc tcggcaaccg aggtgccgtc
      181 cgttccaccc cgcaatgcca gctcgggctc gggccaggag agcttgcgca tgtcttccat
      241 ctcgtcgtcc cgaagatagg gcggattcga gacgatgagg tcgaaccggc ctgggacgcg
      301 ctccagcagg tcggagtggt acgcggggaa cgggttcgac aggagacgcc gcgcgttcat
      361 ctccagcacc tcgagggcct ggggcgagag gtcggaggcg ctgaccacga gctgcggcat
      421 ggcgttctgc aggctgatcc cgacgcaccc ggatccggcg caggagtcgt ggactcgccg
      481 cagccgcgga tccccgcgcg ccacgcgcag cgccttctcc acgaggacct cggtgtccgg
      541 gcgcggcacc agcacgcgct cgtccacgta gaaatccagg ccgtagaact ccttctgccg
      601 gcgcagatag gagaccgggg ttcccgcgca tcgacgatcg atcagcgcct ggaaggcatc
      661 ctcgatcccc tctcccaccg gatccggcag ggaggccagg agcttctcct tggtcagggc
      721 cgaggcatgg gccaggagca cgaccgcatc caggagcggc gtttccacct ccgcgtagaa
      781 cagcttgtcg tagccctgct tgagcaggga ccgggccgtc atgggctctc cgccgccagg
      841 gccgactcct gcgagctcag gcgcagcgcc tccacgaact cgtcgatctc tcccttgacc
      901 accgcgtcca gcttgtagag ggtcaggttg atccggtgat ccgtcgcccg gttctgcggg
      961 aagttgtacg tgcggatctt ctccgcccgg tcgccggatc cgatctggct cttgcgcgcc
     1021 tgggcgcgct cggcgtgctt gcgctcctcc tcagcctcgt agaggcgggc ccgcaggatc
     1081 cgcagcgcct tggccttgtt cttgtgctgg ctcttctcgt cctggcagtg caccacgatg
     1141 cccgtgggca ggtgggtgat ccgcacggcg gagtccgtgg tgttcacgct ctgccccccc
     1201 ggaccgccgg cgcgcatcac gtcgatccga aggtcattcg ggttgatctc gatctccgtc
     1261 tcctcgacct cggggagaac ggccacggat gccgtggaag tatggatccg accgccggcc
     1321 tccgtgaccg ggacgcgctg gacccggtgc accccgctct cgtaccgcag ccgcgcgtag
     1381 acccccttgc ccgagatggc ggagatgatc tccttgaagc ctccgatccc cgtggggctg
     1441 gaggacatga tctccacctt ccacttctgg cgatccgagt agtagctgta catccgaaag
     1501 aggtccgcgg caaagagcgc cgcctcctcc ccaccggtac ccgcccggat ctccacgatg
     1561 acgttcttgt catcgagggg atccggcggc accaggaggg acttcagccg ctgctccgag
     1621 gccaggcggc gctcctcgag ggcacgaagc tcctccttgg ccatctcgcg aagctcgcgg
     1681 tcctccgagg tctccgccag ctccgtgttg gaggagatct ccgccgccag cccccggtag
     1741 cggtcgtact ccgccaccag ctcgctcagg gcggagtgct cctgccgcat ctcgcggaac
     1801 cgcttctggt ccttgacgac ctccgggtcg gcgagctgcc cttccagctc ctgatgtcgc
     1861 gcgcgcgcct tgtccagctt ctcgaacact cgttgtcccc tgatcccaga ttagcagaaa
     1921 cggcggacca tcgccaccag accggtcatt gctccagaag cgctcgaagc tccttcgtgc
     1981 gagcatccac caccgcggcg ccgcgcccct ccagacggag ccgaagcagc ggctccgtgt
     2041 tgctgggacg gacgttgaac cagtagtccg gaaactccac ggagatcccg tccagccggt
     2101 ccatcttgcc cccggcgtag cggcgctcga tctcccgcat ggcccgctcc ttgtccttga
     2161 cctccacgtt gatctcgggg gactgcacgt accggctgcg caggggatcg accagggccg
     2221 agagaggccg gccctcctgc gccaggaagc gcagcaacga ggccagcgcg tacgccgcgg
     2281 actccgtgta gtaggtgtcg ctcacgcgga agtagacgtg cccggaggtt tccgcgccga
     2341 aggccgcacg ggaggctacc atctggtcgt agaggaacgt gtatcccacg cgggagacca
     2401 cgggccggcc gcccatctcc gttacccgct cggggagcac ccgggatgag atcaggtcat
     2461 agacgatgga ggttcccgcc tggcgccgca gaataccctc cgccaccaga caggagagga
     2521 agtagttctc gatcccccgt cccttctcgt ccacgaacag gatgcgatcg ccgtcgccat
     2581 ccaggatgac gcccaggatg gcccccgcct ggacaacggc ctcggccgcc tgccgccgag
     2641 actcctcttt caaggggttg gggtcgtggt tggggaaggt gccatcgggg gtctcgttca
     2701 ggagcctcgc gtcgacgccc agggcgtttg ccagggcccg gaactccttt cccccggagc
     2761 cgttggaggc atcaatcacg acggggccgg cgggtacctt accctgcccg gaacccgcga
     2821 tgaaccggat gtaggcctca agggtatcct tctccgggaa cgaccgggtc gggacggtcc
     2881 tgacctccgg gaccttgtcg aacagcgcct ccacgtcttt cagccccttg tcgtaggaca
     2941 gggatccccc ggtggcgtcg aaaaccttga acccgtggta ttccttgggg ttgtgagacg
     3001 ccgtgaccat cacggcggcc tcgtacttgc catccatctg ggagtagtgc agcatcgggg
     3061 tgctgcacag gccgaccccg gttacctggg cgccctcctc cagcaggccg ctggccaggg
     3121 cctgatagag ctccctggag tgggcgcgag cgtcgtaccc caggagccag cctttggcct
     3181 tgcggtaccg caagagcgtc ctccccaggc ggtaggcaaa cggcaggtcg atgccctgct
     3241 cccagacccc acgtatatcg taggttttga atacactcat atagatacga tatcaccaag
     3301 gttcggtgga agtgaagctc cggcgcccgg taccgggtgg atcggggtcg gggttgacac
     3361 ctccggagtc gctgggtaag ttaagcacgg cccgctcgcc tttggaatcc tcctggtcgg
     3421 acaacttggg gccccagccc ccgtggtatg gaagggagac atgggaccga ccctaatcgg
     3481 tttgctccag gaatcggtcg tgaagtattc atccctagcg gcactcaagt ctcgggggac
     3541 ggacggcaac taccagattc tgacttacgc agagctctac accgcggtga gcgagctcgg
     3601 caccggcctg atctccgtgg gcctggcggc gggcatgcac gtcgcgctgg ttgcggagaa
     3661 cggcccccgc tggctcatct cggacctggc aatcctggga atcggaggag tggacgtccc
     3721 cgtttcaacg ggggtggcgg acacggagct ggagcaggtc gtcgcccatg gggactgcga
     3781 gatggcggtg gtggacaacg ccggcaccct gtcccggctc cttgccatgc gaaagcgcat
     3841 cccgagggtc cgcaggatcc tggtcctgga cttcacgggg cccaaaccca gtgccggaac
     3901 gggtgaggag cgggttctcg tctacacctg ggaagagatg atccagaagg gacgaacgcg
     3961 gatcgcgcga gggcagcgca cgtttgatct gcgggccgcc agcgtgactc ctgcggatcc
     4021 ggccaccctc ctgtacacct ccgggaccac gggaaagccc aagggcgtga tgctgtccca
     4081 tggcaacttc atgcacaacg tcaccaccat ccacgccagc ataagcccgc ggccgggctc
     4141 ggcctggctt tccgtcctgc cggtctggca ctccttcgag cgcacggtgg agtactgcag
     4201 catctacttc gggagcacca tcgcctactc ccaggcggcg gaatggcgtc tgctggagga
     4261 cctggggacc ctgggaccca actacctggt gctgatgccg gccctcctgg aaacgctcgc
     4321 aaaaagcatc gagaagcggc tctccttcgt agagaacctc ctggtgcgct tcgagaagtt
     4381 ctacctcgtc ttctccggct tcatcatggg ccgctatccc cgattccggc gggaagagcg
     4441 cctgctcgag atcttcgcgg cggtcccggc gctcctgact ctctctccca tcaagctggt
     4501 atccgccctg accctccgca ggaagatccg gtccctcctg ggcggggcac tgagggcgat
     4561 cgtctacggg ggcggtccct tgccggccta cctggatcgc ttctttgccg ccatcggcgt
     4621 ggacgtgctg gaggggtatg ggctgacgga ggcatccccc atcgtcagcg ttcggtccga
     4681 gcgggcaccg gtcctgggga ccgcgggcag gccccttccg tcgacggagg tacggatcgt
     4741 cggggacaag ggcgaagtac tgcccccggg ccgcaagg
//
LOCUS       DASRZS010000079         4288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4663103, whole genome shotgun sequence.
ACCESSION   DASRZS010000079 DASRZS010000000
VERSION     DASRZS010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4663103"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /locus_tag="VFH83_01935"
     CDS             complement(<1..1794)
                     /locus_tag="VFH83_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485147.1"
                     /translation="MRRAALSLLLLIGTASLAQAQQIKEIEFRNQPITDILLALGEMA
                     GRSIVPDETVTGTASYYFTATDFDTALKIFLSTYKLFSWRDGAIYYVSRVLAQSSKDT
                     GAATVDADDVDLRLIIRALSRAIGKTILFDTLPRENLTIHAVDTPPAVVLEILLKRFP
                     DYKVDSSKDYFYVRKVDAAAGPATASGGTGSPVTVAGGRYSLDVDKARFRDVLVDLFA
                     KAGLEYSLFLRSDVILENLHFADKSLDEILRLVMEQANADYTVASGIYYVYDIQRTDV
                     MKKLKTVRQIPLSYVSVQDLPNLFPQEMASQNLYRLDRNSNSIILTGSQEEIGPIEEF
                     VHAIDQPTDGKDYCRFDLSFLKVSDLPSVLPPSLSGIKPIALPQGNSFVMLLSPASEK
                     MLQEYLPLVDRKEPAHAIQLKYVQSDFLLKNLPPSVSKDDVQQTGDATLVFFTGSDDR
                     LRQFQREIDLLDRPAPQIRYELLVVEYQNGQGLDWSLKLNANATPSALPGAFLGSIDR
                     LLNLNFNIVSTFGYLFAIELNLGLTENKANILADTSLTGLSGQEIKFQNTETTRHQEL
                     EVDPDTGKLLPTGVTREVTTGLIIGMNGWVSG"
     gene            complement(1791..2240)
                     /locus_tag="VFH83_01940"
     CDS             complement(1791..2240)
                     /locus_tag="VFH83_01940"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HET6485148.1"
                     /translation="MLPWWRIAAFAAFAIPITLVDLRRMRIPDALSLGGIVVVLALDV
                     LLLKAPALSIVLEAAVGFGVFWLLRLGTRGKLGFGDVKYSVLIALSLGLLGWLTTIAV
                     ASVTGLIAALVLIRRLRVPLSTRIPFAPFLTFGAAVSIVMRQARFLS"
     gene            2678..3790
                     /locus_tag="VFH83_01945"
     CDS             2678..3790
                     /locus_tag="VFH83_01945"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HET6485149.1"
                     /translation="MALRILLASDLHIGMRFVEYEAIQTKLTAERLACLGRIVEEAGR
                     RRADLLVIAGDLFHRVNVPRRDVVQAAETLSAFSGGLVVVLPGNHDFLSADDELWPRF
                     REASGNGVLFLQYPKPYPLSAYDLDACLYPGPCGSIHSKENAVGWVRGTQRDPAVKHH
                     IGIAHGSLEGISPDFNGDYYPMRPAELMEMGLDLWLLGHTHLRYPESPGKNDRIFLPG
                     VPEPDGFDCTHDGYCWDITLSDDGGVSAEPVRTGGFRFVEEKVDVRGTEDLDALESKY
                     AEVEAGSILLRVRLSGRVSHETLAAVGPLGARMADRLLHLDLHAEDLREEITLESIDR
                     DYAQGSFPHLLLSKLAESKDVEGLQLALELLEEVKE"
     gene            3787..>4288
                     /locus_tag="VFH83_01950"
     CDS             3787..>4288
                     /locus_tag="VFH83_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6485150.1"
                     /translation="MILTRVRAAPFGCFKDQEVRFGPGMNVVLGPNEAGKSTIYRAIR
                     HALFVPSKLKKNQNAQYLDPYLPAGGGDSIVVGVDLESAGQKWLLRRRWGARPTSEMT
                     LPAGGKISDESAIQEQLSEILPARQTTFVNVLMTGQAELAGTIAMLREKGKDALHDLA
                     DVLRQAV"
BASE COUNT          794 a         1279 c         1444 g          771 t
ORIGIN      
        1 accggaaacc cagccgttca tcccgatgat gagaccggtc gtgacctccc gtgtcacgcc
       61 ggtcggcagg agcttgcccg tgtccgggtc cacctccagc tcctggtgtc gcgtagtctc
      121 cgtgttctgg aacttgatct cctggcccga gagcccggtc aaggaggtat cggcgaggat
      181 attcgccttg ttctcggtga gccccaggtt cagctcgatg gcaaagaggt agccgaaggt
      241 ggacacgatg ttgaagttga ggttgagcag gcgatcgatg ctccccagga acgcgccggg
      301 aagcgcggac ggcgtggcgt tggcgttgag cttcaagctc cagtccagcc cctggccatt
      361 ctggtactcc accaccagga gctcgtatcg gatctgaggc gcgggccgat ccagaaggtc
      421 gatctcccgc tggaactgcc gaagcctgtc gtccgatccg gtgaaaaaca cgagtgtggc
      481 atcccctgtc tgctgcacgt catccttgga gacggacggc ggcaagttct tcaggaggaa
      541 atccgattgg acgtacttga gctgaatggc gtgggccggc tctttgcggt ccaccagcgg
      601 gaggtactcc tgcagcatct tctcggacgc ggggctgagg agcattacga acgagttgcc
      661 ctgcggaagg gcgatgggct tgatcccgct caaggacggt gggagcaccg aagggagatc
      721 gcttaccttg aggaagctga ggtcgaagcg gcagtagtcc ttgccgtcgg tgggctggtc
      781 gatggcgtgg acgaactcct cgatgggccc aatctcctcc tggctccccg tcaggatgat
      841 gctgttggag ttgcggtcca ggcggtagag gttctgggac gccatctcct gggggaacag
      901 gttgggcagg tcttgcacgg agacgtagga gagcggtatc tggcggacgg tcttcagctt
      961 cttcatcacg tccgtgcgct ggatgtcgta gacgtagtag ataccgctgg ccacggtgta
     1021 gtccgcgttt gcctgctcca tcaccagtcg aaggatctcg tcgagcgatt tgtccgcgaa
     1081 gtggaggttc tccaggatca cgtcggaacg aaggaagagc gaatactcca gccccgcctt
     1141 ggcgaagagg tccaccagga cgtcgcggaa gcgggccttg tcgacgtcca gggagtagcg
     1201 gccgccggcg acggtgaccg gggagcccgt gcccccactc gcggtcgcgg gcccggcggc
     1261 ggcatccacc ttgcggacgt agaagtagtc cttgctggaa tccaccttgt agtcggggaa
     1321 tcgcttcagg aggatctcga ggaccacggc agggggcgtg tccaccgcgt ggatcgtcag
     1381 gttctcccgg ggcagcgtgt cgaagaggat ggtcttgccg atggcgcgag agagggctcg
     1441 gatgatgaga cgaaggtcca cgtcatcggc gtccaccgtg gcggcgcccg tgtccttgct
     1501 gctctgcgcg agcacgcggg aaacgtagta gatggcgccg tcccgccagg agaacagctt
     1561 gtacgtggag aggaagatct tcagggccgt gtcgaagtcc gttgccgtga agtagtagga
     1621 ggcggtgccg gtcaccgtct cgtccgggac gatcgagcgt cccgccatct cgcccagggc
     1681 caggaggatg tccgtgatcg gctggtttcg aaactcgatc tccttgatct gttgcgcctg
     1741 ggcgaggctg gctgtaccga tcaggagcaa cagagacagc gctgctctcc tcatgaaagg
     1801 aacctcgcct ggcgcatgac gatggaaacc gccgccccga acgtaaggaa aggcgcaaac
     1861 gggatccgcg tgctgagggg gaccctgaga cggcggatca agaccagggc cgctatcaga
     1921 cccgtcacgg aggcgacggc gatggtcgtc agccatccta gaagcccgag ggacaacgcg
     1981 atcagaacgg agtatttcac gtcgccgaag cccagcttgc cgcgggtccc gaggcggagt
     2041 agccagaaca caccgaagcc gaccgccgct tccaagacga tagagagtgc cggggccttc
     2101 aggaggagca catcgagggc aaggacgacg acgataccgc caagggaaag cgcgtccggg
     2161 attctcatgc gcctcagatc gaccagagtg atggggattg cgaatgccgc gaacgcggcg
     2221 atgcgccacc acggaagcat ttttattcta ccagttctat gcgattggtt tcttcaacca
     2281 atgatgccgt gtatcagtgc gacgcacttt ctgatggtgc ccgcatgata tcacgagaat
     2341 cgctgatttg tcaaatccaa acgacgcgat tccaggagag gcacgccgcc ctcgccaaat
     2401 ctacggaaac gcaccgtgcg atttcgtcga aatcgagagg gtgggcatga gactcaccgc
     2461 cgtccgagcg gagccaccgc cttgttgttg tggcgtagcg gttcggaggc aacgctagac
     2521 cgttgcgcta ccttgaggag cagcagtggg cgagggacga atgggccttc ctggaatgct
     2581 acggcagcag tctgaaaacc ggcggctgag gggtcctccg ggcggaaagg agaggcacgc
     2641 tttaccctcc cgccgccatt ccctatactc gtgagcgatg gccctgcgca tcctgctggc
     2701 ctctgacctg cacattggca tgcggttcgt cgagtacgag gccatccaga ccaagctcac
     2761 ggccgaacgg ttggcatgcc tcgggcggat cgtcgaggag gccgggcggc gccgggcgga
     2821 tcttcttgtc atcgccggtg atctcttcca tagggtgaac gtgcccaggc gggacgtggt
     2881 gcaggccgcg gagacgctga gcgcattcag cggcggtctg gtggtggtgc tgccgggcaa
     2941 ccacgatttc ctctcggcgg acgacgagct gtggccgcgc tttcgggagg ccagcggcaa
     3001 cggcgtcctc tttctccagt accccaagcc gtaccccttg agcgcctatg acctggacgc
     3061 ctgcctctac ccggggccgt gcgggtccat ccactccaag gagaacgccg tcggttgggt
     3121 ccgcggaacg cagagagacc ccgcggtgaa acaccacatc gggatcgcgc acggaagcct
     3181 cgagggcatc tcgccggact tcaacgggga ctactacccc atgcgccccg cggaactgat
     3241 ggagatgggt ctggacctgt ggctccttgg ccacacccac ctccgttatc ccgaatcgcc
     3301 gggtaagaac gatcggatct tcctgccggg cgtgccagag ccggacgggt tcgactgcac
     3361 ccatgacgga tactgttggg acattacgct ctcggatgac ggcggcgtga gcgcggagcc
     3421 ggtgcgcacc ggcgggtttc gattcgtcga ggagaaggtg gatgtgcgag gcacggaaga
     3481 cctcgacgcg cttgagtcga aatacgcgga agttgaggct ggatccatcc tgctgcgcgt
     3541 tcggctttcc ggacgcgtct cccatgagac gcttgccgcc gttggcccgc tgggggcgag
     3601 gatggcggat cggcttctcc acctggatct tcacgcggag gatcttcggg aggagatcac
     3661 cctggagagc atcgatcgcg actatgccca gggatcgttc ccgcatctgc tcctgtccaa
     3721 gctcgcggag agcaaggacg tcgagggcct ccagctcgcc ctggagctcc tggaggaggt
     3781 caaggagtga tcctcacgcg ggtgcgggcg gcgccgttcg gttgcttcaa ggaccaggag
     3841 gtccggttcg gccccgggat gaacgtggtg ctcggcccca acgaggccgg caagtccacg
     3901 atctatcgcg ccatccgtca cgcgctcttt gttccgtcca agctcaagaa gaaccagaac
     3961 gcccagtacc tcgaccctta cctccccgcc ggcggcgggg attccatcgt ggtgggcgtg
     4021 gacctggagt ccgccgggca gaagtggctg cttcgtcggc gctggggcgc cagaccaacc
     4081 agtgaaatga cgctgcccgc gggcggcaag atctcggacg agtcggccat ccaggagcag
     4141 ctctccgaaa tcctgccggc gcgccagacc acgtttgtca acgtcctcat gaccgggcag
     4201 gccgagctgg ccgggaccat cgcaatgctg cgggagaagg gcaaggacgc cctgcacgac
     4261 cttgctgacg tcctgcgcca ggcggtgc
//
LOCUS       DASRZS010000080         5118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3891555, whole genome shotgun sequence.
ACCESSION   DASRZS010000080 DASRZS010000000
VERSION     DASRZS010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3891555"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..799
                     /locus_tag="VFH83_01955"
     CDS             125..799
                     /locus_tag="VFH83_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485151.1"
                     /translation="MGTSTMSPTLRRGALVIYTSDSPGTQPVTVNFQYNPGELRRTLT
                     SRRPRDAAQPSAAPQDARLVAGPPVEAISLSVELDAADQLADPSSNQDTVDHGLHMPL
                     AALEMLLYPPSAQFEHNQQLAQNGQIQVSPSVLSLVLLDWGESRMVPVMVTSFSVTEQ
                     EFDTQLNPIRAKVDLGLQVLTYLDLRDNSTGVDAFVSYQKEKERLARGYYTGSTPAQT
                     QNLSPD"
     gene            812..1102
                     /locus_tag="VFH83_01960"
     CDS             812..1102
                     /locus_tag="VFH83_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485152.1"
                     /translation="MFLKGSRYASVPENVLVDPRGRVLRYKTTRFLRPSAAQVGHILS
                     DGERVEHVAYFYYRDPERFWRICDANQAMDPEELNHAPGRKIGIPASEDKGG"
     gene            1112..2227
                     /locus_tag="VFH83_01965"
     CDS             1112..2227
                     /locus_tag="VFH83_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485153.1"
                     /translation="MPAISYTLTLNGNPLDAVVLGKLRKVECEEHAGMADLLRLTFAL
                     TQKDDGSGWLVLDDNVFQRLGKLTLSITVGSGGAQDILEAYVVEVTAEFANDPAGNAL
                     SVVAFDPSVLMNLEEKARAWTNMADSDIASSIFGAYGFGTDVSATQPSRTENALTVVQ
                     RGSDIQFLRQLARRNGYECYVTLDPTSGAATGHFHAPRYGASPQGVLSVGMGRDSTIE
                     TLRITYDEARPAQTQTANIDLFSSGVQTAQVTSVSSPTLGRTSTLPSDQPRKVLVNPL
                     GLTDAGELQTYAQAVADRSAVAIRAEGELNAQAYQGVLRARGTVLVRGVGPSLSGTYY
                     VERVLHTFEASSYKQGFTLRRNAVGVDGQETFAMEST"
     gene            2224..2829
                     /locus_tag="VFH83_01970"
     CDS             2224..2829
                     /locus_tag="VFH83_01970"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HET6485154.1"
                     /translation="MSDNLFESAFQRQWFGKFRATVTDNNDPEALGRLKCRVPALGDV
                     ELGWALPCVPYAGSKVGFYMVPDPQANVWVEFEGGDPSRPVWVGAFWTADQKLDNAVP
                     DVKVLKTNAHTISLDDTSGSEKIEIKHRSGTVITIDTNTLTITHSGGASLTMDSSSIA
                     IDNNGPKATLDSSGVSLENGGQKVAVGTSSVTINGTSLEVL"
     gene            2839..3168
                     /locus_tag="VFH83_01975"
     CDS             2839..3168
                     /locus_tag="VFH83_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485155.1"
                     /translation="MPAILQQGAVIQCPHGGTVTAVPTNAVVRVNGAFALLVSDLFLV
                     AGCSFVIGTTPSPCVTVEWQLPATKIAINGTAVLLDSSLGLCKGPTQAVQGTALISGV
                     QTKVDGM"
     gene            3179..3538
                     /locus_tag="VFH83_01980"
     CDS             3179..3538
                     /locus_tag="VFH83_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HET6485156.1"
                     /translation="MPQFMDYPFAIDGRGRVRTTDRDDHIRDMIYLVLFTQPGERLNR
                     PDFGCGLGARPFEPNSRTQAAATQFLVKSALQRWLSDVISVEAVSVEAQDERLVVNVD
                     YVRLDNGQRQSDAFAAL"
     gene            3549..>5118
                     /locus_tag="VFH83_01985"
     CDS             3549..>5118
                     /locus_tag="VFH83_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485157.1"
                     /translation="MTRFHDGIAIQDDARRGDLQQDPTLNGIDYLEVDTSPQGDNERV
                     LRVHFLPDQSPAPQTKLDLLWTDILSAGVMIDISGGTRIRNVQVIERHRVGDVLVLRV
                     DLPGDYSDYTLTIDTDRLDPYYRQVVFNFKAGCPSRFDCKASEYCPPESRPAPAIDYM
                     AKDYGSFRQALIDLIPTLAPDWTERRAADFGISLVELLAYAGDQLSYQQDAVANESYL
                     ETARQRISVRRHARLIDYGMHDGLSATTFLQLTITGLAPVTVAGRAVIRAVALPRALQ
                     QPASRLPLELQDAALREADAVFEVILGHDQKLFLSSQLNTIPLYTWGNRQVFLPRGAT
                     SADLLGNLDLAAAPGESWRLKPGDYLLFEEVKDPQSGQPQDANPEHRQVVRLTSVRGD
                     TDPLFNQAITHVEWANADALSFPLCVAAVNEQTQAVIDSISVARGNLAVAFHGQSIHQ
                     GYPAELPPPGVAPVVPGIDPDASTRGYRFPLEKGPLSILPGPAPDSTGTPTAPQAVHD
                     LQRSSPRLGYPAVMD"
BASE COUNT          930 a         1719 c         1682 g          787 t
ORIGIN      
        1 gcagcggtag ggcaggccga gagagcgcgg gatgccatgg tcgcactggc tgccgaggcc
       61 ccgacccaca agtacggaga acgggcccgc gactacctga gcaggcccga gaaggagtga
      121 gccgatgggc acctcgacga tgagcccgac cctgcgaagg ggcgccctgg tgatctacac
      181 gagcgattcc cctgggacgc agccggtgac cgtcaacttt cagtacaacc ccggtgagct
      241 gcgccgaacg ctgaccagcc gccggccgcg ggacgccgcc cagcccagcg ccgctcctca
      301 ggacgcccgc ctggtggccg gtccaccggt ggaagcgatc agcctttccg tcgagctgga
      361 tgccgccgac cagcttgcgg acccgagcag caaccaggac acggtcgacc acggtctgca
      421 tatgccgctt gccgccctgg agatgctcct gtatccgccc agcgctcagt tcgagcacaa
      481 ccagcagctc gcccagaacg gccagatcca ggtctcgccg tccgtcctgt ccctggtgct
      541 gctggactgg ggggagtccc ggatggtgcc tgtcatggtg accagcttct ccgtcaccga
      601 gcaggagttc gacacccagc tcaaccccat ccgcgccaag gtggacctgg gtctccaagt
      661 gttgacctac ctcgatctgc gcgacaacag cacgggcgtg gacgccttcg tttcctacca
      721 gaaggagaag gagcgcctgg cgcgcggcta ctacacgggc agcaccccgg cgcagaccca
      781 gaacctcagc ccggactaga gaggagaggc catgttcctg aagggcagcc gatacgcctc
      841 cgtacccgaa aatgtgctgg tagacccgcg cggacgcgtc ttgcggtaca agaccacgcg
      901 cttcctgcgc ccttccgcgg cgcaggtggg gcacatcctg agcgatggcg agcgcgtgga
      961 gcacgtggcg tacttctact atcgcgaccc tgagcgcttc tggcgcatct gcgacgccaa
     1021 ccaggcaatg gacccggagg agctcaacca tgcgccgggc cgcaagatcg ggatccccgc
     1081 ctctgaggac aagggcgggt agggggcgga gatgccggcc atctcgtaca ccctgacgct
     1141 caacggcaac cccctggatg ccgtggtcct cgggaagctc cgcaaagtgg aatgtgagga
     1201 gcacgccggg atggcggatc tgctgcgcct gacctttgcc ctgacccaga aggacgatgg
     1261 cagcggctgg ctggtcctgg acgacaacgt gttccagcgg ctgggcaagc ttacgttgtc
     1321 catcaccgtc ggttccggtg gagcgcagga catcctggaa gcctacgtag tggaggtgac
     1381 cgcggagttc gccaacgacc cggctggcaa cgctctcagc gtcgtggcgt tcgacccgag
     1441 cgtcctgatg aacctggagg agaaggcgcg cgcctggacc aacatggccg actccgacat
     1501 cgccagctcg atcttcggcg cctacggttt cggcacggat gtcagcgcga cgcaaccgtc
     1561 gcgaacggag aacgcgctga cggtggttca gcgcggcagc gacatccagt tcctgcgcca
     1621 gctcgcccga cgcaacggct atgagtgtta tgtcacgctg gatccgacca gcggggccgc
     1681 aacgggccac ttccacgcgc cacgctacgg cgcatcgccc cagggggtcc tttccgtggg
     1741 gatgggccgc gacagcacga ttgagacgct gcggatcacc tatgacgaag cgcgcccggc
     1801 ccagacccag acggccaaca tcgacctgtt ctcctccggc gtgcagaccg cccaggtcac
     1861 cagcgtctcg tcacccaccc tggggcgtac ctcgacgctg ccctccgatc agccgcgcaa
     1921 ggtgctggtg aacccgctcg ggctgaccga tgccggcgag ctgcagacct atgcgcaggc
     1981 cgtggcggac cgctccgcgg tcgccatccg agccgaggga gagctgaacg cgcaagccta
     2041 ccagggagtg ctgcgggccc gcggcacggt gctggttcgc ggcgtggggc cgtcgctcag
     2101 cggaacctac tacgtggagc gcgtgctgca caccttcgaa gccagtagct acaagcaagg
     2161 cttcaccctg cggcgcaatg ccgtgggcgt ggacggccag gaaaccttcg cgatggaaag
     2221 cacatgagcg acaacctgtt tgagagcgcc ttccagaggc agtggttcgg gaagttccgc
     2281 gctacggtga ccgacaacaa cgatcctgaa gccctgggtc ggctgaaatg tcgggtccct
     2341 gcccttgggg acgtggagct gggatgggcg ctgccctgcg ttccctacgc gggcagcaag
     2401 gtgggtttct acatggttcc ggacccccag gcgaacgtat gggtggagtt cgagggcggg
     2461 gacccttctc gtccggtgtg ggtgggagcg ttctggacgg cagaccagaa gctggacaac
     2521 gccgtgccgg acgtgaaggt gctcaagacc aacgcgcaca ccatatccct ggacgacacc
     2581 agcggcagcg agaagatcga gatcaagcac cgtagcggca ccgtgatcac catcgacacc
     2641 aacaccctca ccatcactca cagcggcgga gccagcctga ccatggactc ctcctccatc
     2701 gccatcgaca acaacgggcc gaaggcgacc ctggactcct ctggagtgag cctggagaac
     2761 ggcggacaga aggtcgcggt tgggacctcc tcggtgacga tcaacgggac gtctctcgag
     2821 gtcctgtagg aggatccgat gccggccatc ctgcagcagg gagccgtgat ccagtgcccg
     2881 cacggcggaa cggtgaccgc cgtcccgacg aacgccgtgg tgagagtcaa cggcgcgttc
     2941 gctctgctgg tctcggacct gttcctggtg gcgggctgct cgtttgtcat cggcaccacg
     3001 ccctctccgt gcgtgaccgt ggagtggcag cttcccgcca ccaagatagc gatcaacggc
     3061 accgcggtgc tgctggatag cagcctgggc ctgtgcaagg ggcccacgca ggccgtgcaa
     3121 gggacggcat tgatcagcgg cgttcagacc aaggtggacg gaatgtagga ggccggccat
     3181 gccccagttc atggactatc cattcgccat cgacggaagg ggtcgggtcc gcaccaccga
     3241 ccgcgacgac catatccgcg acatgatcta cctcgtgctg ttcacccagc cgggcgagcg
     3301 gctcaatcgg cccgatttcg gctgcggcct gggcgcgcgg cccttcgagc ccaacagccg
     3361 gacccaggcc gccgctaccc agttcctggt caagtcggcg ctccagcgct ggctgtcgga
     3421 cgtgatcagc gtggaagccg tctccgtgga ggcgcaggac gagcgcctcg tggtcaacgt
     3481 cgactacgtg cggctggaca acggccagcg gcagagcgac gcctttgcag ccttgtagga
     3541 acgcaagcat gacccgattc catgacggca tagcgatcca ggacgacgcc cgtcggggcg
     3601 atctccagca agaccccacg ctcaacggga ttgactacct ggaggtggac accagcccgc
     3661 agggcgacaa cgagcgtgtg ctgagggtcc atttcctgcc ggaccagtct ccggcgcctc
     3721 agaccaagct ggacctgcta tggaccgaca ttctgtccgc cggagtgatg atcgatatca
     3781 gcggcggaac gcggatacgg aacgtccagg tgatcgagcg gcatcgggtg ggtgacgtcc
     3841 tggtgctgcg ggtggacctg ccgggggact actccgacta cacgctgacg atcgacaccg
     3901 accggctgga tccttactac cggcaggtgg tcttcaactt caaggcaggc tgcccgagtc
     3961 ggttcgactg caaggcgtcg gaatactgtc cacccgagtc gagacccgcc ccggccatcg
     4021 actacatggc caaggactat ggcagcttcc gtcaggcgct gatcgacttg atcccgacgc
     4081 tggctccgga ctggacggag cggcgcgccg cggatttcgg gatttccctg gtggagctgc
     4141 tcgcgtacgc cggggatcag ctcagctacc agcaggacgc ggtcgccaac gaatcctacc
     4201 tggagacggc ccggcaaagg atatcggtgc gcaggcacgc ccgcctgatc gactatggca
     4261 tgcacgacgg tctgagcgcg accaccttcc tccagctcac gatcaccggc ctcgcgcccg
     4321 tgaccgtggc cggccgggcg gtgatccgcg cggtagcgct gccgcgcgcg ctgcagcaac
     4381 cggcaagccg gctccccctg gagctgcagg atgcggccct ccgggaggcc gacgcggtct
     4441 tcgaagtgat cctgggccac gaccagaagc tgttcctctc cagccagctc aacacgatcc
     4501 cgctctacac gtggggcaac cggcaggtct ttctcccgcg cggggcgacc agcgcggacc
     4561 ttctggggaa cctggacctc gccgccgcgc cgggagaatc gtggcggctg aagccgggcg
     4621 actacctgct cttcgaggag gtgaaggatc cgcagagcgg tcagccgcag gacgcaaatc
     4681 ccgagcaccg ccaggtcgtc cgcctgacca gcgtgagagg agatacggat ccgcttttca
     4741 accaggcaat cacccacgtg gaatgggcca acgccgatgc gctgagcttt ccgctgtgcg
     4801 tggctgcagt caacgagcag acccaggcgg tgatcgacag catcagcgtg gcgcgcggaa
     4861 acctggccgt cgcttttcat gggcagagca tccaccaggg ctatccggcg gagttgccgc
     4921 cgccgggagt cgccccggtg gtcccgggca ttgaccccga cgcctccacg cgcgggtatc
     4981 gcttcccgct cgagaagggt ccgctcagca tcctcccggg gccggctcct gattcgaccg
     5041 gcacgccgac cgcgccccag gccgtgcatg acctgcagcg aagctcgccg cgcctcgggt
     5101 acccggcggt catggacg
//
LOCUS       DASRZS010000081         3121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_814019, whole genome shotgun sequence.
ACCESSION   DASRZS010000081 DASRZS010000000
VERSION     DASRZS010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814019"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VFH83_01990"
     CDS             <1..640
                     /locus_tag="VFH83_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6485158.1"
                     /translation="VDLGAGPLSPTTGKLAENLRGAGYGPRDIDTVLITHAHPDHVGG
                     TLDGAGSLRFPDARYFVWRTEWDYWFSEAAEKVSWEHIDFTAIARRNLAPLKPHLTLV
                     EDEREILPGVAMVNAAGHTPGHVIICFSSRGETLAYAADTAIYPLHLEHPGWLPVFDM
                     LPDQAAASKRRIFDRAAAEKWLVMAQHFPPFPSLGHIQKRDEGWTWVPALGE"
     gene            1074..2693
                     /locus_tag="VFH83_01995"
     CDS             1074..2693
                     /locus_tag="VFH83_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485159.1"
                     /translation="MLHAYVDVDGDGVPGTGEPQGYYPDSQDAMPYQNDASDLSIQMV
                     NRVASGSISVLSYSESYTDYRQITNGTVIDSILVIKPAVNGVSVFVTDDTGHYTLLGV
                     PAGSYTITPAYASSAYARTVTVRSGASSSVTVLTPAWTVMVYVVNGAVSSPPLNNPDV
                     RRALSASLDRAQILSSLGWSDCTPGTNLLPPTQSLGTWADGASAVSCSPALADSLLSN
                     VSPFSLTVSVSSTQSALAFFAGAQPFLAQPTAVTAVTSDVATASEIAARCAAGAFQIA
                     RFGWGFDSNNLLVFYEAVLGTNGACNYGHYSSTALDDLIAEGRAALAVGDLSGYQDCI
                     LELNSKILKDMPIIPLYWIVPDPSPGAGQTPRVIPTVASGAIAIDGSPGDWSGMSPAI
                     ADPVGDSGITGIGTDITALYLARDDTYLYWRMDLADGTANPQFAYEIWIGDETQNWAS
                     GEHDYEVRLRYDSVGSSWEPVIFQKQGTTWQYIGVDPSFGAFASVCEMKVPLSYLGAY
                     GPDVYIRGHIQRTSGDVDPDFTSYLLFDVGS"
     gene            complement(2701..>3121)
                     /locus_tag="VFH83_02000"
     CDS             complement(2701..>3121)
                     /locus_tag="VFH83_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485160.1"
                     /translation="GVGSMPWIFLFLLGCLAAMALVMLAAIWGMNRATHWMVGNKHHL
                     LEGIFDTGDVPQEWSRRTAALLAKASRAAADPRDAARAARIHEQAKASYLHRLDALIR
                     YARDTRIVRDEETRRVLLDRLASVRREWSERKAEELR"
BASE COUNT          526 a         1042 c          952 g          601 t
ORIGIN      
        1 cgtggatctg ggggccggcc ccctgtcccc caccacgggg aagctggcgg agaacctccg
       61 gggcgcgggg tatgggccgc gggacatcga caccgtcctc atcactcacg cccacccgga
      121 tcatgtcggc ggaaccctgg acggcgcggg cagcctccgc ttcccggacg ctcgctactt
      181 cgtctggcga acggaatggg actactggtt ctccgaggct gcggagaagg tgagctggga
      241 gcacatcgac ttcaccgcga tcgcgcggag gaacctcgcc cccctgaagc cccacctgac
      301 gcttgtggag gatgagcggg agatcctgcc cggcgtggcc atggtcaacg ccgccggaca
      361 tacaccgggg cacgtcatca tctgcttctc ttcccgggga gagacgctgg cctatgcggc
      421 ggataccgcg atctacccgc tccacctgga gcaccccggc tggttgcccg tcttcgacat
      481 gctgccggac caggcggccg cgagcaagcg ccggatcttt gaccgggcag cggctgagaa
      541 gtggctggtc atggcccagc acttcccgcc gttccccagc ctggggcaca tccagaagcg
      601 tgatgagggg tggacctggg tgcccgcgtt gggcgaatag cacgagcggg tccgcaacaa
      661 cttctctgcg agcctattgt cttgacagcc cagcaccacc cgtccaatgg ttgtatctgg
      721 ttcagaagta cctgtcagaa gtacctgatt gataattcgt gtttggaggc gagaaagtga
      781 aaaccagcag gtgcggggtt gccgtccgtg cggccgtagt cctggcgttt gtcgcaagct
      841 gctgttcctg cgcatttctg gaggacctcc tcggtttggg aaccgtttcc atagcgggaa
      901 ccgtaagcgt gccgagagac ctgactgtca gccggccgac cacggtgcat gttcgactac
      961 tcacggctgc cctccaggcc gtgtcattcc caggcggcga ctctaccgca tctttcacgg
     1021 gcgccaactg gcttcttcct ttttcgtttc ctaaggtccc cagaggggac tacgtgctgc
     1081 acgcttacgt cgatgtggac ggtgatggtg tgcctggtac cggtgagccc cagggctact
     1141 acccggattc gcaggatgcc atgccgtatc agaacgacgc ttccgacctt tcgatacaga
     1201 tggtcaaccg ggtcgcctcg ggttccatca gcgtgctctc gtactctgag agctacaccg
     1261 actaccgtca gatcacgaac gggaccgtca tcgattccat cctggtgatc aaacccgccg
     1321 taaacggcgt ttccgtgttc gtcacggacg acaccggtca ctacaccttg ttgggcgtgc
     1381 ctgccgggtc gtacaccatc acgccggcgt acgcgtcctc ggcatacgcg cgtaccgtca
     1441 cggtccgcag cggcgcctcc tcctccgtca ccgtgctcac tcccgcgtgg actgtcatgg
     1501 tgtacgtggt caacggcgcc gtctcctctc caccgttgaa caacccggat gtccgcaggg
     1561 ccttgtccgc atccctggac cgtgctcaga tcctcagcag tctgggatgg tcggattgca
     1621 ctccaggcac caatctactg cctccgactc agtcgctcgg aacatgggcc gatggcgcct
     1681 cggctgtttc gtgttcgccg gccctggcag atagcctgct gagcaacgtg tcgcccttca
     1741 gtctgaccgt ctccgtatcc agtacccaga gcgcactggc cttcttcgcc ggggcacaac
     1801 ccttcctggc tcaacccacg gccgtgacgg cggtgacctc cgatgtcgca acggcctctg
     1861 agatcgcggc gcgctgcgcc gcgggagcgt tccagatcgc cagatttggt tggggcttcg
     1921 actcgaacaa tctgctggtc ttctacgagg ccgtcctggg cacgaacgga gcgtgcaatt
     1981 acggtcacta ctccagcaca gctctggacg atctcatagc cgaaggcagg gctgcccttg
     2041 cggttgggga tctctccggc taccaggatt gcatcctcga gctgaacagc aagatcctta
     2101 aggatatgcc gatcatcccg ctctactgga tcgtgccgga tccctccccg ggcgcaggcc
     2161 agacgccccg cgtcatcccg accgtggcat ccggtgccat cgcgatcgac ggcagcccgg
     2221 gagactggtc cgggatgtcg cctgccattg ccgacccggt cggagactcc gggatcaccg
     2281 gcatcggtac cgacatcacc gccttgtacc tggcccgtga tgacacctac ctgtactggc
     2341 gcatggatct ggcggatggc accgcgaacc cgcagtttgc gtacgagatc tggattggcg
     2401 atgagacgca gaactgggcc agcggcgagc acgactatga agtcagactg cgatacgact
     2461 cggtgggttc ttcctgggag ccggtgatct tccagaaaca gggaaccacc tggcagtaca
     2521 tcggcgtgga tccgtccttc ggggcgttcg cttcggtctg cgaaatgaag gtccccctca
     2581 gctacctggg cgcctacgga cccgacgtct acatccgggg ccacatccag cggacgtctg
     2641 gcgacgttga tccggatttc acgtcgtacc tgttgttcga cgtcggtagc tgaaggcgga
     2701 ctaccgcagc tcctcggcct ttcgctccga ccactcccgc cgcaccgagg caagacggtc
     2761 cagcaggacg cgacgcgtct cctcgtccct cacgatgcgg gtgtctcggg cgtagcggat
     2821 cagggcgtcc agccgatgga ggtagctggc cttggcctgc tcgtggatgc gggccgcgcg
     2881 ggcagcatcc cgcgggtccg ccgccgccct cgaagccttc gccagcagag ccgccgtcct
     2941 ccggctccac tcctggggga cgtcacccgt atcgaagatc ccttccagca ggtggtgctt
     3001 gttcccgacc atccagtggg tggcccggtt catgccccag atggctgcca gcatgaccag
     3061 cgccatggcg gccaggcatc cgagcaggaa caggaagatc cagggcatcg atcctacgcc
     3121 c
//
LOCUS       DASRZS010000082         8272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774 k141_43249,
            whole genome shotgun sequence.
ACCESSION   DASRZS010000082 DASRZS010000000
VERSION     DASRZS010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43249"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFH83_02005"
     CDS             complement(<1..293)
                     /locus_tag="VFH83_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019008255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6485161.1"
                     /translation="MAAEPAVWTRDLQKTYVVTEREAGARAALRSLVRRNREQVHAVD
                     GISFELAPGEIVGFLGPNGAGKTTTLKMLSGLLHPTGGSLAVLGHEPWKRERS"
     gene            419..2725
                     /locus_tag="VFH83_02010"
     CDS             419..2725
                     /locus_tag="VFH83_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET6485162.1"
                     /translation="MTTDREKKNLPENQPPEPSRPRDAERAVRLELLAQVGRRTTAIL
                     SLEDLLRSSAQIVAETFSYFMVSIFLAEGNDLVLRACTLPVFSDAIKTLRIGIGKEGI
                     TGWVAAHGEPLNVPDVREEARYHYEIVEELQTRSELAVPILLQGAVIGVLDAQSTRTG
                     AFSGLDAFTLQTVADQLAVAIENARLYGKLREELATRERQERMLLAFHRAGLAMADAK
                     SPEAFLESVGGPLDGIGLSCTVYRTDDAPGMATLAYTSFGKELLGTVEQVLGRSPIGL
                     SFAVQEVEAFRSIAAGGPALFVAAGSVDGSLFPVPHTEAQARIARILSFPNTIVVPLA
                     AEDRLLGFLAAHSPELTPQDIPVLRLFADQVATAWRKAQLVLDLQNSVEELRRAQEQL
                     LQSQKMEAVGRLAGGVAHDFNNHLTAILGYTDLLAETLGPQDTQRMELGEIRKAALLA
                     ADLTRQLLAFSRKQMLQPRVVDFNLLIADVKGMLRRLITEDIRLETVLSRDPVPARAD
                     PSQIQQVIVNLALNGRDAMPGGGVLTIATQVVTDAGASGVDSPESSGEYSVIRVTDTG
                     TGMDRETRSRLFEPFFTTKARGKGTGLGLSTAYGIVTQSGGRITCESEPGRGTTFTIL
                     LPTAHEAAEPVQPQPAQAPRSGDGELVLVVEDESAVRELVQRTLEGNGYRVLTAATGR
                     EGMDLMIANPVDLLLTDLVLPGGMSGLELARYALESEGRIRLLCVSGYSERLAAGDGS
                     ILPPRAFLPKPFTPAGLLARVREVLDSE"
     gene            complement(2765..3790)
                     /locus_tag="VFH83_02015"
     CDS             complement(2765..3790)
                     /locus_tag="VFH83_02015"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET6485163.1"
                     /translation="MSGKRLGIAEALREGIAEEMRRDQRVFCIGEDIGIPGGWGGAFT
                     VTLGLEKEFADRLINTPISEAGLVGAALGAAMMGMRPIADVQYADFLFCAMDQVANQV
                     AKMRYMSGGKLETPMVLRAPVGVTGRGAQHAQMMEPYFIPLPGIKIVCPATAYDAKGL
                     LKAAVRDGNPVLMFEHKLLYGSKGPRSETGALDASSDIPEEDYTVPIGKGIIRREGSD
                     VTIVASLLMMHRAMEAAKLLAQEGISAEVIDPRTLVPFDWDLVKGSVAKTGRVITVEE
                     SPRRGGVGGEIAATLAEEMIDYLIAPVKRVAAPNTPAPFAPPMEKFYVPDAAKIAKAA
                     RDLMAIS"
     gene            complement(3787..4755)
                     /locus_tag="VFH83_02020"
     CDS             complement(3787..4755)
                     /locus_tag="VFH83_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020753732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET6485164.1"
                     /translation="MKAQTPDPKWMLELYRQMVLIRQFEERVKFLFLEGVMPGTIHQY
                     QGQEACAVGVCAALGPQDVVTSTHRPHGHAIARGLSVGSMMAELFGKSTGCCKGKGGS
                     MHMGDLSLGMVPAVAIVGGALPMATGIGLAFKMRSEKRVAVAFMGDGATNEGSFHEAL
                     NMAAIWDLPVVFVVENNLYGASTPVKMVVKTPHISDRASAYGMPGCTIDGNDVLAVHA
                     TASQAVERARGGGGPTLLELETYRITGHSRRDPCLYQPEEERKRAVANEPIGRFAKYL
                     LGSKLAAPGDLDAIQESVKAEVEAAVQKAMSDPDPLPEDTLEGLFV"
     gene            complement(4752..5933)
                     /locus_tag="VFH83_02025"
     CDS             complement(4752..5933)
                     /locus_tag="VFH83_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET6485165.1"
                     /translation="MATPLRMPDLGTVEGAVTLVSWLKAEGDSVALGEPLFEVETDKG
                     VSEIEAAIAGVIVKLVIPAGGKAGPGELIAMIRRPGEPEETAEAPPEGRPATASTPTA
                     AAPAAAILPTVVTPAAAGAVPPTIRALAARYGVELSAVRGSGPGGRILREDVLAARKG
                     PGTGPAPLAAAAQPAPPAAPSRNQAAVARVVSQSHREKPTFHLTMTVDMSRVIEARKS
                     AKPASIAFDAYLVKAVARAIASSPGFRRYQKDEKLLSHDAVDVAVAVSVDDDLYAPAI
                     RRADTKSVRQIAGDIDALAAKARARAIGTAESEGSCFLVSNLGMFPVESFDAIVYPDH
                     AAALAVGAAIPTPVSDGTRIWIAPLARLTLTVDHRLINGAVAARFLAAVKQFLESGDL
                     S"
     gene            complement(5933..6916)
                     /locus_tag="VFH83_02030"
     CDS             complement(5933..6916)
                     /locus_tag="VFH83_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6485166.1"
                     /translation="MYKGNKLGACLPTFGSAADRYVLQGYGRGGNTMEEMLAMSKKVQ
                     GLDGLELVGNWHINDKNIAAVQKLFRDNGLAVAMVVPDLWTQAKWGRGSLAAAEAAVR
                     KEAVAEVKRCMDWAAEAGCAYVDVWPGQDGYDYPFEADFTDAWKWMKDGVAECAAHSK
                     KVKVLVEYKLREPRNRCFISTAAKVILLLQGIPNTGCLLDVGHSLAAGENMAEATALL
                     AEFGILDYIHLNDNYRTWDDDMIASSIHVPEALELFYWLKRVKYQGWLTLDIFPYREE
                     KIPAAEESFAWMRALIDRVEAKGLDAITKVVRKGEGTDSVKLVREMLLGQR"
     gene            complement(7022..>8272)
                     /locus_tag="VFH83_02035"
     CDS             complement(7022..>8272)
                     /locus_tag="VFH83_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HET6485167.1"
                     /translation="LEGEKFIEHFSLIQALALEGSTGTWEKVEEDTTDVRIKLSGKLV
                     GYFEIPNEDPFTKEAVVQLAFPIDAWIDNVPMMLLSIAGNCFAYSKYMRLLDVFMPPN
                     LLKNFKGPKFGVPGLRELTGVKDRPLSLHIIKPKMGMTPEQVGAQVYQTAIGGVDLCK
                     DDEMTSDVYNSNYLDRIKAVNAQLEKAEAKTGKHVIYFCSITHEPAELVKRARKAVAE
                     GARGLLICYSAGLAALREVAEDPKVNVPILLHASHMIAAQPRIAWPVFAKLCRICGAD
                     LMLTPTYWSSIPMVSMEEGIRTSHMKLAPLGHIKPIFPMPCAGVYPGLAPILVGEYGS
                     DIVIPAGGGMLGHPDGYTAGAKAWQQAIAAAMAGQNIIEFAKKPENRELRRAIEKWGV
                     IDRPKTPWLRVAPQFHPHKMKFDE"
BASE COUNT         1256 a         2861 c         2729 g         1426 t
ORIGIN      
        1 aagctccgct cacgcttcca gggctcgtgc cccaggaccg cgagagagcc gccggtggga
       61 tgcagcagtc cggagagcat cttgagcgtg gtggtcttgc ctgccccgtt cggtcccagg
      121 aaccccacga tctccccggg agccagctcg aagctgatgc catccaccgc gtggacctgt
      181 tcgcggttcc ttcggaccag gctccgcagg gccgcgcgag cgcccgcctc cctctcggtc
      241 accacgtagg tcttctggag gtcgcgggtc cagactgccg gctcggctgc catcgggtga
      301 atatacgacc ggtcccatgc gggctcaaga gcgcggagca cggcttgacc cgcttcagcg
      361 tcgatcatac tgttgagggg tccgctgtgg cccttgccgg gcgcgcgtct cttcacgaat
      421 gaccaccgat cgcgagaaga aaaacctgcc cgagaaccag ccccccgaac cgtcacgacc
      481 gcgcgacgcg gagcgggcgg tgcgcctgga gctcctggcc caggtgggac ggcggaccac
      541 ggcaatcctt tccctggaag acctccttcg cagctccgcc cagatcgtcg cggagacgtt
      601 ctcctatttc atggtgtcca tcttcctcgc cgaagggaac gacctcgtcc tgcgggcgtg
      661 cacgctcccc gtcttttcgg acgcgatcaa gacccttcga atcggcatcg gcaaagaggg
      721 catcacgggg tgggtcgcgg cgcatggaga acccctcaac gttccggatg tgcgtgagga
      781 ggcccgctac cattacgaga tcgtcgagga gcttcagacg cggtcggagc tcgccgtgcc
      841 gatcctgctc cagggcgcgg tgatcggcgt cctggacgcg cagagcacgc ggaccggcgc
      901 cttcagcggc ctggacgcct tcacgctaca gacggtggcg gaccagctcg ccgtggccat
      961 cgagaacgcc cggctgtacg gcaagctgcg cgaagagctc gccacgcgcg aacggcagga
     1021 gcggatgctc ctcgcgttcc atcgagccgg gctggccatg gcggacgcga agtcgcccga
     1081 agcgtttctg gagagcgtgg ggggacccct ggatgggatc ggcctctcgt gcacggtcta
     1141 ccggacggac gatgccccgg gcatggcgac tctggcatac accagcttcg ggaaggagct
     1201 tctgggtacg gtggaacagg tcctgggcag atcccccatc gggctctctt tcgccgtcca
     1261 ggaggtcgag gcctttcggt cgatcgcggc cggcggcccg gccctgttcg tcgcggccgg
     1321 atccgtggac gggtccctct tccccgtgcc gcacaccgaa gctcaggcac ggatcgctcg
     1381 gatcctctca ttcccgaaca ccatcgtggt ccccctggcg gcggaggacc ggctcctggg
     1441 gttcctcgcg gcgcactctc ccgagctgac gccgcaggac atccccgtgc tgcggctgtt
     1501 cgcggaccag gttgccaccg cctggcgcaa ggcgcagctc gtccttgacc tccagaacag
     1561 cgtcgaggag cttcgccgcg cccaggagca gctcctccag tcgcagaaga tggaggcggt
     1621 cggcagactg gcgggcggcg tggcgcatga cttcaacaac cacctcaccg ccatcctcgg
     1681 gtacacggat ctccttgcgg agacgctcgg tccccaggac acgcagcgca tggagctggg
     1741 ggagatccgc aaggcggcgc ttctggccgc ggatctgacg cgtcagctcc tggcgttcag
     1801 ccgcaaacag atgctccagc cacgggtagt cgacttcaac ctgctgatag cggacgtgaa
     1861 ggggatgctg cggcgcctga tcacggagga tatccgcctg gagacggtcc tgtcccggga
     1921 tccggtcccc gcccgcgccg atccctccca gatccagcag gtcatcgtga acctggccct
     1981 gaacggacgg gacgccatgc ccgggggcgg tgtcctgacc atcgctaccc aggttgtcac
     2041 ggacgccggg gcgtccggcg tggactcgcc ggagtcctct ggagagtact ccgtgatccg
     2101 cgtgacggat accggcacgg gtatggaccg ggagacgcgc agccggctct tcgagccgtt
     2161 cttcacgacc aaggcccgag gcaaaggcac gggcctgggg ctctcgaccg cttacgggat
     2221 cgtcacccag agcggcggcc ggatcacctg tgagagcgag cccggtcgtg gcaccacgtt
     2281 cacgatcctg cttcccaccg cgcacgaggc tgccgaaccc gtccagccgc aaccggcgca
     2341 agccccgcgg agcggcgacg gcgagctggt gctggtggtg gaagacgagt ccgcggtccg
     2401 cgagctggtc cagcgcacgc tggagggcaa cggctaccgg gtgctcacgg ccgccacggg
     2461 acgggaaggc atggacctga tgatcgccaa tccggtggat ctgcttctca cggacctcgt
     2521 ccttcctgga gggatgagcg ggctggagct tgcgcgctac gcgctggagt cggagggcag
     2581 gatccgtctg ctctgcgtct cagggtattc cgagcgactc gcggcgggtg atgggtcgat
     2641 cctgcctcca cgggcgtttc tgccgaagcc cttcacgccg gcgggtcttc tggcccgggt
     2701 tcgcgaggtc ctggactccg agtaggagcc gatccatcgc ggctctcgag ggagggacgg
     2761 cgccctacga gatcgccatc aggtcccgtg ccgccttcgc gatcttcgcc gcgtcgggca
     2821 cgtagaactt ctccatgggg ggagcgaacg gcgcgggcgt gttgggcgcc gccacccgct
     2881 tcacgggggc gatcaggtaa tcgatcatct cctccgccag ggtcgcggcg atctctcccc
     2941 cgacgccgcc gcgtctgggg ctctcctcta ccgtgatgac ccgccccgtc ttggcaacgg
     3001 agcctttcac caggtcccag tcgaagggca ccagcgtccg agggtcgatg acctccgcgc
     3061 tgattccctc ctgggccaga agcttcgccg cctccatggc gcggtgcatc atgaggagcg
     3121 aggcaacgat ggtcacgtcc gatccttccc gccggatgat ccccttgccg atgggcaccg
     3181 tgtaatcctc ttcggggata tcgctggagg cgtccagggc tccggtctcc gaacgcggtc
     3241 ccttggagcc atagaggagc ttgtgctcga acatcagcac cgggttgccg tcccgcaccg
     3301 ccgccttcag cagccccttg gcgtcgtacg cggtggccgg gcacacgatc ttgattcccg
     3361 gcagcgggat gaagtacggc tccatcatct gcgcgtgctg ggccccgcgt cccgtgaccc
     3421 cgaccggcgc gcgcaggacc atgggggtct ccagcttgcc gccggacatg tagcgcatct
     3481 tggcgacctg gttggccacc tggtccatgg cgcagaagag gaagtccgcg tactgcacgt
     3541 ccgcgatggg gcgcatcccc atcatggcgg cccccagcgc cgcgccgacg agccccgcct
     3601 cggagatggg cgtgttgatc agtcgatcgg cgaactcctt ctccaggccc agggtcaccg
     3661 tgaaggctcc gccccatcca ccggggatcc cgatgtcctc cccgatgcag aacacacgct
     3721 ggtcccgacg catctcctcg gcgatgccct ctcgcagcgc ctccgcgatc cccaacctct
     3781 taccgctcat acgaacagac cctccagcgt gtcctcgggc agcgggtccg gatccgacat
     3841 ggccttctgg accgcagcct ccacttccgc cttcacgctc tcctgaatcg cgtccaggtc
     3901 tcccggggcc gcgagcttgg atcccaacag gtatttggcg aaccggccga tgggctcgtt
     3961 ggccacggcc cgcttgcgct cctcctccgg ctggtagagg cacgggtccc gacgggagtg
     4021 gccggtgatc cgataggtct ccagctccag cagcgtcgga ccgccgccgc cccgggcccg
     4081 ctccacggcc tgggacgcgg tggcgtggac cgcgagcacg tcgttgccgt cgatggtgca
     4141 gccgggcatc ccataggccg atgcccggtc ggagatgtgc ggcgtcttca cgaccatctt
     4201 cacgggggtc gaagccccat acaggttgtt ctccaccacg aagaccacgg gcagatccca
     4261 gatggcagcc atgttgagcg cctcgtggaa cgacccctcg ttggtggcgc cgtcccccat
     4321 gaacgcgacg gccacccgct tctccgatcg catcttgaac gcgaggccga tccccgtggc
     4381 catggggagg gcgccgccca cgatggccac ggcgggcacc atgcccagcg aaaggtcgcc
     4441 catgtgcatg gagccgccct tgcccttgca gcagccggtg gacttgccga agagctccgc
     4501 catcatggat cccacgctca gacctcgggc gatggcgtgc ccgtgggggc ggtgcgtgga
     4561 ggtgaccacg tcctggggcc ccagggccgc gcagacgccg acggcgcacg cctcctggcc
     4621 ctggtactgg tggatggtgc cgggcatgac gccctccagg aagaggaact tcacgcgctc
     4681 ctcgaactgg cggatgagga ccatctgccg atagagctcg agcatccact tcgggtccgg
     4741 tgtctgtgct ttcacgagag gtctcccgat tccaggaatt gcttcaccgc cgccaggaac
     4801 cgcgcggcga cggcgccgtt gatcagacgg tgatccacgg tgagggtgag acgcgccagg
     4861 ggcgcgatcc agatgcgcgt gccgtcggat accggcgtcg ggatggcggc gccgaccgcg
     4921 agcgccgcgg cgtgatccgg atacacgatg gcgtcaaagg actccacggg gaacatcccg
     4981 aggttgctga ccaggaagca gctcccctcc gactcggcgg tcccgatggc gcgggcccgg
     5041 gccttggccg ccagagcgtc gatgtcgcca gcgatctgac ggacgctctt ggtgtccgca
     5101 cggcggatgg cgggggcgta cagatcgtca tcgaccgaga cggcgaccgc cacgtccacg
     5161 gcatcgtggg atagaagctt ctcgtccttc tggtagcggc ggaaccccgg gctcgacgcg
     5221 atggcgcgcg ccaccgcttt caccaggtac gcgtcgaagg cgatggaggc cggcttggcg
     5281 ctctttcgcg cctcgatgac gcggctcatg tccaccgtca tggtgaggtg gaaggtcggc
     5341 ttctctcggt ggctctggct gaccacgcgc gcgacggcgg cctggttgcg ggagggcgcg
     5401 gctgggggag caggctgggc ggccgccgcc aagggtgctg gccccgtgcc cgggcccttt
     5461 ctcgctgcca gtacgtcttc ccgcaggatc ctgccgcccg gcccgctgcc gcggaccgcg
     5521 gacagctcca cgccgtagcg ggccgccagg gcgcggatgg taggaggcac cgcgccggcg
     5581 gcggcaggag tcaccaccgt gggcaggatc gcggcggcgg gtgccgcggc cgttggtgtc
     5641 gaagccgtgg cgggtcgccc ctcgggggga gcctccgcgg tctcctccgg ttctcccggc
     5701 cgccggatca tcgctatgag ctccccggga cccgccttgc cgcccgcagg gatgaccagc
     5761 ttgacgatga ccccggcaat cgccgcctcg atctcgctca cccctttgtc ggtctccacc
     5821 tcgaagagcg gctcgccgag ggcgacgctg tccccctcgg ccttgagcca gctcaccagg
     5881 gtgacggcac cttccacggt gcccagatcc ggcatccgaa gcggcgttgc cattatcgct
     5941 gtcccaggag catctcgcgg accagcttca cgctgtccgt gccctctcct ttgcgcacca
     6001 ccttcgtgat ggcatccagg cccttggcct cgacgcggtc gatgagcgcg cgcatccagg
     6061 cgaaggattc ctcggccgcc gggatcttct cctcacggta cgggaagatg tccagcgtga
     6121 gccaaccctg gtacttcacg cgcttcagcc agtagaagag ctccagggcc tcgggaacgt
     6181 ggatcgacga agcgatcatg tcatcgtccc aggtccgata gttgtcgttg aggtggatgt
     6241 agtccaggat cccgaactcc gccagcaggg ccgtggcctc cgccatgttc tctccggcgg
     6301 ccaggctgtg acccacgtcc aggaggcacc ccgtgttggg gattccctgg agaaggagga
     6361 tcacctttgc cgcggtgctg ataaagcacc ggttgcgcgg ctcccgcagc ttgtactcca
     6421 ccagcacctt gaccttcttc gaatgcgcgg cgcattccgc cacgccgtcc ttcatccatt
     6481 tccaggcgtc cgtgaagtcc gcctcgaacg ggtaatcgta gccgtcctgc ccgggccaga
     6541 cgtccacgta ggcgcatccc gcctccgccg cccagtccat gcagcgcttg acctcggcaa
     6601 ccgcctcctt gcggacggcg gcctccgcgg cggcgaggct ccccctgccc cacttggcct
     6661 gggtccacag gtccgggacc accatggcca ccgccaggcc gttgtcgcgg aagagcttct
     6721 gcacggcggc aatgttcttg tcgttgatgt gccagttgcc aaccagctcc aggccgtcga
     6781 ggccctggac cttcttggac atcgccagca tctcttccat ggtgttgccg ccgcggccgt
     6841 acccctgaag gacgtagcgg tccgccgccg agccgaacgt cggaagacat gcgccgagct
     6901 tgttcccctt gtacatacgg taccccctgg gccgcgttgc gcggcctccc aggccgcatc
     6961 gggctggcct gctgccctat cgggtggcgc cccgcttctc cctcggggcg ccaccgttgg
     7021 atcactcgtc gaacttcatc ttgtgcggat ggaactgcgg cgccacacgc agccacggag
     7081 tcttgggccg atcgatgact ccccacttct cgatggcgcg gcgcagctcg cggttctccg
     7141 gcttcttcgc gaactcgatg atgttctgtc cggccatggc cgccgcgatg gcctgctgcc
     7201 atgccttggc tcccgccgtg tagccgtccg gatgccccag catgccaccg cccgccggga
     7261 tgacgatatc cgaaccgtac tctcccacca ggatgggcgc cagcccagga tacacgcccg
     7321 cgcacggcat ggggaagatg ggcttgatgt gtccgagcgg ggccagcttc atatgggagg
     7381 tgcggattcc ctcttccatg ctgaccatcg ggatggacga ccagtaggtg ggcgtgagca
     7441 tgaggtccgc gccgcagatc cggcagagct tggcgaacac gggccaggcg atgcgcggct
     7501 gagcggcgat catgtgggac gcgtgcagca ggatggggac gttgaccttc ggatcctcgg
     7561 cgacctcccg cagcgcggcc agacccgccg agtagcagat caggagcccg cgggcccctt
     7621 ccgccactgc ctttcgagcg cgcttcacca gctcggcggg ctcatgggtg atgctgcaga
     7681 agtagatgac gtgcttgccg gtctttgcct ccgccttctc gagctgggcg ttgaccgcct
     7741 tgatccggtc caggtagttg ctgttgtaca cgtcggaggt catctcgtcg tccttgcaga
     7801 gatccacgcc tccgatggcg gtctggtaga cctgggcgcc cacctgctcc ggcgtcatgc
     7861 ccatcttggg ctttatgatg tgcagggaca ggggccgatc cttcacgccg gtgagctccc
     7921 gaaggccggg cacgccgaac ttggggccct tgaagttctt caggaggttg gggggcatga
     7981 acacgtccag caggcgcatg tacttcgagt acgcgaagca gttgccggcg atggacagaa
     8041 gcatcatcgg cacgttgtcg atccaggcat cgatggggaa ggcgagctgg accaccgcct
     8101 ccttggtgaa cgggtcctcg ttggggatct cgaagtagcc caccagcttg ccggagagct
     8161 tgatccgcac gtcggtggtg tcttcctcca ccttctccca ggttccggtg gaaccctcca
     8221 gggccagggc ctggatcagg gagaagtgct cgatgaactt ctctccttcc ag
//
LOCUS       DASRZS010000083         6768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_430346, whole genome shotgun sequence.
ACCESSION   DASRZS010000083 DASRZS010000000
VERSION     DASRZS010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430346"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..326
                     /locus_tag="VFH83_02040"
     CDS             192..326
                     /locus_tag="VFH83_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485168.1"
                     /translation="MFRSDLDVQAAAVVLEALTRGRAMLTMVSPDALADERINAEIGR
                     "
     gene            complement(429..650)
                     /locus_tag="VFH83_02045"
     CDS             complement(429..650)
                     /locus_tag="VFH83_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017294231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HET6485169.1"
                     /translation="MENRKEEIVFEVLQEQDGGFVAECLGESIITEADTWEELRNNVR
                     EAVSAYFFDDAPPARIRLHLVRDEVLTVA"
     gene            1210..1617
                     /locus_tag="VFH83_02050"
     CDS             1210..1617
                     /locus_tag="VFH83_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HET6485170.1"
                     /translation="MKIVVLYCQHCVTYGKESPIAPREHDGVVVQTVMMPCSSKVQAS
                     EVLKLLAAGADGVEIVACSDESCRLLVGSRMAEKRLQYAQRLLNEVHVPKERLGFSWK
                     SGVGPEELEAIGLRRAFAVEASTVEAPGGGEET"
     gene            1614..2285
                     /locus_tag="VFH83_02055"
     CDS             1614..2285
                     /locus_tag="VFH83_02055"
                     /inference="COORDINATES: protein motif:HMM:NF023646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HET6485171.1"
                     /translation="MIFSEWKPLDEIVRQLDGRKKVLLVGCATCVAECAAGGEREVET
                     LAPLLSLAFREKGQPIEIATCTLEKQCEWEFVEELSGKATGVDALLSLACGIGVQALA
                     ERFPELPTYPGVNTSALTIREEAGIWASRCAACGDCVLGETFGYCPMARCAKSLMNGP
                     CGGTRENGKCEINETLDCVWNLIVERAELRGELESLGRVRGPKSWSNSRHGGPKRVIR
                     EDLRA"
     gene            2282..3217
                     /locus_tag="VFH83_02060"
     CDS             2282..3217
                     /locus_tag="VFH83_02060"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HET6485172.1"
                     /translation="MKTKVGSNLERVLAAGHFAVTAEVGPPRGPNPEAVLKKVKAVKG
                     IADAYNVTDNQTAVVRMSSIAASRLLLESGLEPVMQMVCRDRNRIAMQSDLLGAWALG
                     LRNCLCLSGDHQVAGAGGKLKGHPGAKNVYDVDSVQLLSILRGLRDEARQEGGDAIDC
                     PAPFFIGAAWTPLGPPEKFRAVRLGKKVAAGADFVQTQAVFDIPRFTEAMRRVVDMGL
                     TERVAILPGIILPRSAGMLRYMNASVPGVRVPEALIQRMSSASDPKEEGFKIAVELIH
                     AVRQIPGVKGVHLQAIEAEHLLPQLAERAGLLPRP"
     gene            3281..6262
                     /locus_tag="VFH83_02065"
     CDS             3281..6262
                     /locus_tag="VFH83_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HET6485173.1"
                     /translation="MSSKYHISVKETAPRFDPAGKLNALEREGCLGCRICVKRTSCVY
                     DVYRKRQFNAAQVVDTTDVLCINCMRCVQECKKNILERARNPQYDRMGDDYWRPDLIA
                     SLWKQAETGKIPVSGAGYRGPFVGPGFDSMWTDMSEIVRPTRDGIHGREYISTVIELG
                     RRPAFLEFDAEGRLLSEEPRFVEIPIPMILQIPDGGFVTPSVRKAISQAAGRLGTIAG
                     VPLEDAIGASSDQKGHLMARFDPTKDDPAALGGVPLAEIPYSDRVMEEAAAIRASNPA
                     VVICIRVPLDEHAVARAVALASAGAEVLHLQADRNGRGLGRRRDDFLIQLVREVHLAL
                     LDHSQRDQLTLLVSGGMAMAEHVAKVLPCGADGVVLDTALLVAMECRLCADCSTRAAC
                     PVEIDRVSDGWGANRIVNLIGSWHSQLIEVMGAMGIREVRRLRGELGRVMFFSDLERE
                     NLAPMFGKRSRPDGQTTVDTFKGNLRSVKQDLGRPDWTRPGMGAEIKPCPSRFRNALG
                     MYKVVRTAACTGCGTCADVCRYGVHVKAGKRILAPKAHLCQGSEVCRSEGRFCVSRCP
                     KNAIRVGLDQTWQAFGDPRWPAELLAASWKQAETGYPPEAGLEYRVGESGGGFDRIAL
                     VFPDERPRDSFKPAEVDLRVALNRRKDGRPDVHIGVPFYGGGMSLGSISLVTMLARAA
                     AYTAVDSFTCTGEGGYPEELQAYDDHVITQVATGLFGVREETIQRVRMVEFKYAQGAK
                     PGLGGHLLGDKNTAAVARMREAVQGVSLFSPFPFHSVYSVEDHKKHVDWIKEVNTRAL
                     VSVKVSGASDVDMVAVGSYYAGAHVVHLDGSYGGTGAAPDIAKKNIAMPIEYAVPKVH
                     RFLLAEGVRDQVTLVASGGVRTAWDIAKVVALGANAVVIGTAEMVALECIRCGACESG
                     RGCARGIATTDPELSVLYSSEWAAQRLINLFHSWSIQLQQILWTLGLRSINDLVGRSD
                     VLEHLDYERRQKPAELPGQAP"
     gene            6259..>6768
                     /locus_tag="VFH83_02070"
     CDS             6259..>6768
                     /locus_tag="VFH83_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HET6485174.1"
                     /translation="MSRSVEQILAARGRLIQGLPAAEGSDRVEEGGCGVVGFAASVPV
                     NGRTVFRACMQMHNRGNGKGGGIAAAGLVPDQLGVSAATLRENYLIQIALLVPQVEGQ
                     VEESFLAPDLEIAHKARVAHLEDHREVGLDVRPPEVTRYFVRVKPASLRRFAETNHLE
                     SLQPRALEDE"
BASE COUNT         1226 a         2078 c         2325 g         1139 t
ORIGIN      
        1 gagggactgc gtacgcgcga cgtggctgcc aggagcggtg taacggtggc cacgttgcac
       61 tactacttcc caacgaaaga acacctgatc cgtggcgtca tggccgccgt cgtggagcaa
      121 ttcaggagac cgagaccgac ccatccggac accgcggccc ctgacccggg caagcagggg
      181 tcgagaaggg cgtgttccgc tcggatctgg acgtgcaggc agccgcggta gttcttgagg
      241 cgctgaccag aggaagggcg atgctgacaa tggtgagccc ggatgcgctc gcggatgaac
      301 gcatcaacgc ggagatcggg cgctagtgtc aaggacgatg tgactgcctc gctcatggac
      361 gactcggtag tcgaatcgcg acacaagcag agccgccaga tcccgcccgc tcagatctcg
      421 cggaagcttc atgcgaccgt gaggacttcg tcgcggacga gatgtagtcg tatcctcgcg
      481 ggtggtgcat cgtcgaagaa gtaggcggag accgcttccc tcacgttgtt tcggagttcc
      541 tcccaggtgt ctgcttcggt gatgatactc tcgcccaagc attcggccac gaagccgcca
      601 tcctgctctt gaaggacctc gaaaacgatt tcctcctttc ggttctccac gaagcaaatc
      661 ctacttgagc tcgaagctgg atgcaagcag cgagggtgga ccaagactgc agcgaccaac
      721 gcaaggctcg cgccaggcct cggtcgtcgc accaagacgc aacggcgtcc gcaccgatcg
      781 tacctggaat atcggagcgc ggaagctccg gggtgtccag tcgattacca ggtccccgga
      841 cggtttgtgg atggccgctc atctctgcag acggcggtgc gtcatggacc gatcagccat
      901 ccgtggggat caggaaatgg cggtccctgg catgttccgc cgacggcagc caggtcattg
      961 cctgtgctga ccaggatggg gtgcttgtcg ggcgtcccgt cgaccctgcc cggagcgcga
     1021 agtaatcctc cacagcagcc gaaagcgcca ggcatgcgcg ccagcgagcg aggcggcctg
     1081 gatacttccc ggggctgaca ggctctcaat gtccctcttg gaggtggccg tctggaaggt
     1141 tgacatttcc agatcctcgt gacctacttt tccaaaatcg gaataaatat gcatgtacca
     1201 actcgaggca tgaagattgt cgtgctctac tgccaacact gcgtcaccta cggtaaggag
     1261 agccccattg caccacggga gcacgacggt gtggtcgtgc aaacggtgat gatgccctgc
     1321 agcagcaagg tccaggcatc ggaggtcctc aagctactgg ctgccggcgc agatggcgtc
     1381 gagatcgtgg cgtgttctga tgagagctgc cggcttctgg tggggagccg catggcggag
     1441 aagcggctgc agtatgcgca gcgcctgctg aacgaagtcc acgttcccaa ggagcggctg
     1501 gggttcagct ggaaatccgg ggtggggccg gaggagcttg aagccatcgg gctcaggcgc
     1561 gcctttgcgg tcgaagcgtc tacggtcgag gcccctggag gaggagaaga aacgtgatct
     1621 tttccgaatg gaaaccgctg gacgaaatcg tgcgacagct cgacggacgg aagaaggtgc
     1681 tgttggtggg atgcgccacg tgcgtggcgg aatgcgcggc cggtggagaa cgggaagtgg
     1741 agaccctggc cccgctcctg agcctggcct tccgggagaa agggcagccg atcgagattg
     1801 ccacctgcac gttggagaag cagtgcgagt gggagtttgt ggaggagcta tccggcaaag
     1861 ctaccggcgt ggacgcgctg ctgagcctgg cctgcggcat cggagtgcag gcgctggccg
     1921 agcgctttcc cgagctgccc acgtaccctg gcgtcaacac ctccgcgctg accatccgcg
     1981 aggaagccgg catttgggcc tcccgatgcg ccgcctgcgg cgactgcgtg ctcggtgaaa
     2041 ccttcggcta ctgccccatg gcccggtgcg ccaagagcct gatgaacggc ccctgcggcg
     2101 ggacccgtga aaacggcaag tgcgagatca acgaaacgct ggactgcgtg tggaacctga
     2161 tcgtcgaacg cgccgagctg cgcggcgagc tggagtccct tggcagggtc cgcggcccga
     2221 agagctggtc caactcccgg catggcggcc ccaaaagggt catccgcgag gatctgagag
     2281 catgaagacg aaggtaggca gcaatctgga gcgcgtgctt gccgccggcc acttcgccgt
     2341 gacggcagag gtgggtccgc cgcgtggccc caatcccgag gcggtcctga agaaggtgaa
     2401 agccgtcaag gggatcgcgg acgcctacaa cgtgacggac aaccagaccg ccgtggtccg
     2461 catgtcgagc atcgccgcat cccggttgct gctcgaatcc gggctcgagc ccgtgatgca
     2521 gatggtgtgc cgcgaccgaa accgtattgc catgcagtcc gatctgctgg gcgcctgggc
     2581 tctggggctg cgcaactgcc tctgcctgtc cggtgaccac caggtggccg gcgccggcgg
     2641 caagctgaag gggcatccgg gcgcgaagaa cgtctacgac gtggactccg tccagctcct
     2701 gtccatcctc cgcgggctgc gggatgaggc ccgccaggaa ggcggggacg ccattgactg
     2761 ccccgccccg ttcttcatcg gagcggcctg gaccccgttg ggtccgccgg aaaagttccg
     2821 ggccgtgcgg ctcggcaaga aagtcgcggc aggcgccgat ttcgtgcaga cccaggctgt
     2881 attcgacatc ccccgtttca cggaagccat gcgccgcgtc gtggacatgg gtctcaccga
     2941 acgggtggca atcctgcccg gcatcatcct gccgcggagc gcgggcatgc tcaggtacat
     3001 gaatgccagc gtgccgggag tccgcgtgcc ggaagctctg atccagcgga tgagctcggc
     3061 atcggatccc aaggaggaag gcttcaagat cgcggtggag ctgatccacg ccgttcggca
     3121 gatcccgggc gtgaaaggtg tgcatctgca ggccatcgag gcggagcatc ttctgccgca
     3181 gttggccgag cgcgccggat tgttgcccag accctaatcg aatcccggcc gccggggccc
     3241 gcccaggggc ctggcgcgcg gtcgcatcgg atggtgaaga atgtcgtcga agtatcacat
     3301 tagcgtcaaa gaaaccgccc ctcgcttcga tccggccgga aagctcaacg ccctggagcg
     3361 ggagggctgc ctgggttgcc ggatctgcgt gaagcgcacc tcctgcgtct atgacgtcta
     3421 tcgcaagcgc cagttcaacg ccgcgcaggt cgtggacacc acggacgtcc tgtgcatcaa
     3481 ctgcatgcgg tgcgtgcagg agtgcaagaa gaacatcctg gagcgcgccc gcaatcccca
     3541 gtacgaccga atgggggatg actactggcg gccggacctg atcgccagcc tgtggaaaca
     3601 ggcggaaacg ggcaagatcc ccgtgtcggg tgccggctat cgcgggccct tcgtcgggcc
     3661 gggcttcgac tccatgtgga ccgacatgtc cgagatcgtc cggcccaccc gagacggcat
     3721 ccacgggcgg gagtacatca gcacggtgat cgagctgggc cgccgaccgg cctttctgga
     3781 gttcgacgcg gaggggcgcc tgctgagcga ggagccgcgt ttcgtcgaga ttcccattcc
     3841 catgatcctg cagattcccg acggcggctt tgtgacaccc tccgtgcgaa aggcgatatc
     3901 ccaggccgcg gggcgactgg gcaccatcgc gggagttccc ctggaagacg ccattggagc
     3961 ctcgtccgat cagaagggtc acctgatggc gcgattcgat ccgacaaagg acgatccggc
     4021 ggccctgggc ggcgtgcccc ttgccgaaat cccctactcg gatcgagtca tggaggaggc
     4081 ggctgccatc cgtgcctcca acccagccgt ggtgatctgc atccgggtcc cgctggacga
     4141 gcacgctgtg gcacgcgcgg tagcgctggc gtccgccggg gccgaagtgc tgcacttgca
     4201 ggcggaccgc aacggcagag ggttgggtcg acgccgcgac gatttcctga tccagctcgt
     4261 gcgagaggtc caccttgcac tgctggacca ttcgcagcgg gatcagctca ccctgttggt
     4321 ctccggcggc atggccatgg ccgagcacgt cgccaaagtc ctcccctgcg gcgcggacgg
     4381 cgttgtgctg gacaccgccc tgttggtggc aatggagtgc cggttgtgcg cggactgctc
     4441 gacacgagct gcctgcccgg tggagatcga ccgggtgtca gatgggtggg gcgccaaccg
     4501 catcgtcaac ctgatcggtt cctggcattc ccaactgatc gaagtcatgg gcgccatggg
     4561 catccgagaa gtccggcggc tgcgagggga gctgggccgc gtcatgttct tctccgatct
     4621 cgaacgggaa aacctggcgc ccatgttcgg caagagatcg cgcccggatg gccagacgac
     4681 ggtggatacg ttcaagggga atctgcgctc cgtgaagcag gacctcggcc ggcccgattg
     4741 gacgaggcca ggcatgggag ccgagatcaa gccctgcccg agccggttcc gcaacgccct
     4801 gggcatgtat aaggtcgtgc ggaccgcggc atgcacgggt tgcggcacgt gcgcggatgt
     4861 gtgtcggtat ggcgtgcacg tcaaggccgg caagcgcata ctggcaccca aggcccacct
     4921 ctgccagggg tccgaggtct gccggagcga gggtcgtttc tgcgtgagcc gttgccccaa
     4981 gaatgccatc cgcgttgggc tcgaccagac gtggcaggcg ttcggcgacc cgcgatggcc
     5041 ggccgagctc ctggccgcct cgtggaaaca ggcagaaacc ggctatccgc cggaggccgg
     5101 cctggagtac cgggtaggcg agtcgggtgg cggattcgac cgcatcgccc tggtgtttcc
     5161 ggatgagagg ccgcgggact ccttcaagcc cgcggaggtc gatctgcggg ttgcgctgaa
     5221 ccggcgcaag gatggccggc cggacgtgca tatcggcgtg ccattctatg gcgggggcat
     5281 gagcctgggg tccatcagcc tggtcaccat gcttgcccgt gccgccgcat acacggccgt
     5341 ggactccttc acctgcaccg gagagggcgg ttacccggag gagctccagg cgtacgatga
     5401 ccacgtcatc acccaggtgg ccacggggct cttcggcgtg agggaagaga ccattcaacg
     5461 ggtgcggatg gtggagttca agtatgccca gggagccaag ccgggcctgg gcggccatct
     5521 cctgggggac aagaacaccg cggccgtggc caggatgcga gaagcggtgc agggcgtttc
     5581 cctgttctct cccttcccgt tccattccgt ctactcggtg gaagaccaca agaagcacgt
     5641 cgactggatc aaggaagtga ataccagggc gctggtctcc gtgaaggttt ccggcgccag
     5701 tgacgtggac atggtggccg tcggcagcta ctacgccgga gcgcacgtgg tgcacctgga
     5761 tggcagctac ggcggcaccg gagcggcgcc ggacatcgcc aagaagaaca tcgcgatgcc
     5821 gatcgagtac gcggtaccca aggttcaccg cttcctgctg gcggaaggcg tccgagacca
     5881 ggtgaccctg gtggcctccg gtggagtgcg tacggcatgg gacatcgcca aggtcgtggc
     5941 cctgggcgcc aacgcggtgg tgatcggaac cgctgagatg gttgccctgg aatgcatccg
     6001 ctgcggagcg tgcgagagcg gacgcgggtg cgcccggggc atcgccacga cggaccccga
     6061 attgtccgtc ctgtattcga gcgagtgggc cgcccagcgg ctgatcaatc tcttccattc
     6121 ctggtccatc caactccagc agatcctctg gacgctcggg ctgcgttcca tcaacgacct
     6181 ggtcgggcgc tcggacgtgc tggagcacct ggactacgag cgccggcaga agccggcaga
     6241 gcttccggga caagcaccat gagcagatct gtggagcaga tcctggccgc gcgaggccgg
     6301 ttgatccagg gacttcccgc ggccgaagga agtgatcgcg tggaagaagg cggctgcggc
     6361 gtcgtggggt ttgccgccag cgtgccggtg aatggccgca ccgtgttccg ggcctgcatg
     6421 cagatgcaca accgcggaaa cggcaaaggc ggcggcattg ccgcggcggg gctggtcccg
     6481 gatcagctag gcgtcagcgc cgccacgctg agggagaact acctcatcca gatcgctctg
     6541 ctggttccgc aggtcgaagg gcaggtagag gaatcctttc ttgctcccga tctggaaatc
     6601 gcccacaagg ccagggttgc ccatctggag gatcaccgcg aggtcgggct ggatgtgcgt
     6661 ccgcccgagg tcacgcgtta cttcgtgcgc gtgaagcccg catccctcag gcggttcgcc
     6721 gaaaccaacc acctggagag tcttcagccc cgcgcactgg aggacgag
//
LOCUS       DASRZS010000084         3591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4277760, whole genome shotgun sequence.
ACCESSION   DASRZS010000084 DASRZS010000000
VERSION     DASRZS010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4277760"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFH83_02075"
     CDS             <1..330
                     /locus_tag="VFH83_02075"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HET6485175.1"
                     /translation="FGFFSLVDIELLLKERLRAGTRTTRVLSIVMIYLASLGIYLGRF
                     WRWNSWDILGNPAELMRDLFDRFTAWRAPYRVLGFTILMGTLLNLMYYPVMRRSRTGG
                     CCGSRSG"
     gene            complement(281..1342)
                     /locus_tag="VFH83_02080"
     CDS             complement(281..1342)
                     /locus_tag="VFH83_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6485176.1"
                     /translation="MGRSHARAGHDAGVNGTRRATYRHAGKLFKADTCDDVFRASLRG
                     EIELQSLARGSYPGTRLDEKSLPGVKSLGFWNATREQDWGLRWHRNEGIELTILSSGS
                     LPYFCGDKRYDLRPYDLTIARPWQPHKVGDPAIGPNKLFFFILDVGVRRPNQDWTWPG
                     WILMNRAERDELTKLLRQNELHVWSECRDFHHCFQEIGRAVEEYPKSKDVTYIALKLN
                     ELFYLLLHMFRTRRIPLNESLTSNFRTVQVFLGELRELLTEDWSIERMAKECGVCTTR
                     FVQYCRQLTNQSPMQHLNTLRLERARALLVERRSMPIIDIAMDCGFATSQYFASCFKR
                     KYHVTPSDYRNTRLSLTVA"
     gene            1488..2513
                     /locus_tag="VFH83_02085"
     CDS             1488..2513
                     /locus_tag="VFH83_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET6485177.1"
                     /translation="MKLKRSLIVVFAALLLVALALPAFAGGTAEKKPGAKLKIAYIRP
                     TNEPYYKYGFDGAQMMADKMGVELLGYISDMKPDRELASVEDAITQKVDGIVLMSVSA
                     TSIQSSVNAAYNAKIPIFMLFGYSEDLKDKMVGSVQGDGKLSGGTIGKWVAKNVPEGE
                     VACIMGLPGRGDAEMYRDAFQAEMEKNPKLKLVASVPGDWNRQKAFNQMQNLITSFPN
                     LKAAFVENEDMALGAIQALKEAGKESQVLIVSQNGAPYGLESISAGGIKATVGWSPSQ
                     EAQIALRMLVENVRDKKTPPKLTITPMTVITRDNVSAATPWEPTAASTDATLKMDLSK
                     LQHDFSK"
     gene            2649..3512
                     /locus_tag="VFH83_02090"
     CDS             2649..3512
                     /locus_tag="VFH83_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6485178.1"
                     /translation="MKVGIDSYSYHRYFGEVYDVQKKPAKDMSYEDFLKRAVQLKVDG
                     VSLETCFFQSTDESYLKRLKEIIDRGPLEVVVAWGHPKGFEGGKKPEELADLRRHFTT
                     CRILGAKVMRVVGSSLDYRNDPHGPQIEKLTGIFKECAKIAGDEGVKLADENHYDFTM
                     DEYLGLFEAVGSPHFGMCFDTGNCLRNGDEPVESARRLGRYIFATHTKDVMPLYGGNP
                     KDWFYYACTPVGKGLVNFPGIVAELEKQGYAGLYAVEIDYPYPSIRDEDQAVKESISY
                     LKTLQKSHPKR"
BASE COUNT          695 a         1121 c         1156 g          619 t
ORIGIN      
        1 ttcggattct tcagcctcgt ggacatcgag ctcctgttga aggaacggtt gcgcgcgggc
       61 acgagaacga ccagggttct ctccatcgtg atgatctacc tggcgtcact cggcatctac
      121 ctcggcaggt tctggagatg gaacagctgg gatattctcg ggaatcctgc cgagctgatg
      181 cgcgatctat tcgatcgatt caccgcgtgg cgggcgccct accgcgtgct cgggttcacg
      241 attctcatgg gcaccctcct gaacctcatg tattacccgg tcatgcgacg gtcaaggaca
      301 ggcgggtgtt gcggtagtcg ctcggggtga cgtggtactt gcgcttgaag cagctcgcga
      361 agtattggct ggtggcgaac ccgcagtcca ttgcgatgtc gatgatcggc atgctgcggc
      421 gttccaccag cagcgcgcgc gcccgctcga ggcgcagcgt gttcaggtgc tgcatcggcg
      481 actggttggt gagctgacgg cagtactgca cgaagcgcgt ggtgcagacc ccgcattcct
      541 ttgccatcct ctcgatgctc cagtcctcgg tgagcagctc ccgcagctcg ccgagaaaga
      601 cctgcacggt ccggaagttg ctcgtcaggg actcgttcag cgggatcctg cgggtgcgga
      661 acatgtgcag cagcaggtag aacagctcgt tgagcttcag cgcgatgtag gtgacgtcct
      721 tggacttcgg gtattcctcc acggcccggc cgatctcctg gaagcagtgg tggaagtccc
      781 ggcactcgga ccacacgtgg agctcgttct gccgcagcaa cttggtgagc tcgtcccgct
      841 ccgcgcggtt catcaggatc catcccggcc atgtccagtc ctggttgggc cggcgcaccc
      901 cgacgtccag gatgaagaag aagagcttgt tggggccgat ggcggggtcg cccaccttgt
      961 ggggctgcca gggacgggcg atggtcaggt cgtacggccg caggtcgtat cgcttgtccc
     1021 cgcagaaata cggcagggag ccgctggaga ggatggtgag ctcgatcccc tcgttgcggt
     1081 gccagcgaag gccccagtcc tgctcccgcg tggcgttcca gaacccgagg gacttcaccc
     1141 ccggaaggct tttttcgtcc agccgtgtcc cggggtagga gccgcgtgcc agcgactgca
     1201 gctcgatctc cccccgcagc gacgcccgga agacgtcgtc gcacgtgtcc gccttgaaga
     1261 gcttcccggc gtgccggtag gtggcgcggc gcgttccgtt gacccccgcg tcatggccgg
     1321 cccgggcatg tgacctgccc atttcatgct cctgtataaa atcgaacatt gttccaaatc
     1381 gaacgtgggg agcgactccg gtacccccat actatacatt gaaaccgcac ggtggtgaag
     1441 gagacacccc gttccgatcc accggcgaca cgtacaagga ggatcccatg aaactgaaga
     1501 ggtccctgat cgtcgtgttc gcggcccttc tcctggtcgc cctcgcgctg ccggccttcg
     1561 ccgggggcac cgccgagaag aagcccgggg caaagctgaa gatcgcgtac atccggccca
     1621 ccaacgagcc gtactacaag tacgggttcg acggggcgca gatgatggcg gacaagatgg
     1681 gagtcgagct gctgggctac atctccgaca tgaagccgga ccgcgagctg gccagcgtcg
     1741 aggacgccat cacgcagaag gtggacggga tcgtgctgat gtcagtgagc gccacctcca
     1801 tccagtcgag cgtgaacgcg gcgtacaacg ccaagatccc catcttcatg ctcttcggct
     1861 acagcgagga cctgaaggac aagatggtcg gttccgtcca gggggacggc aagctctccg
     1921 gcggcaccat cggcaagtgg gttgccaaga acgttccgga aggcgaagtg gcctgcatca
     1981 tgggcctccc cggccgcggc gacgcggaga tgtaccgcga cgcgttccag gccgagatgg
     2041 agaagaaccc caagctgaag ctggtcgcat ccgtccccgg tgactggaac aggcagaagg
     2101 ccttcaacca gatgcagaac ctcatcacga gcttccccaa cctgaaggcc gccttcgtcg
     2161 agaacgagga catggcgctc ggcgccatcc aggcgctcaa ggaggcgggc aaggaaagcc
     2221 aggtcctcat cgtctcccag aacggcgccc cgtacgggct ggagtccatc tcggccggtg
     2281 ggatcaaggc caccgtcggc tggtctcctt cccaggaagc ccagatcgcg ctgcggatgc
     2341 tcgtggagaa cgtccgcgac aagaagaccc cgcccaagct gaccatcacc ccgatgacgg
     2401 tcatcaccag ggacaacgtc tccgccgcca cgccgtggga gcccaccgct gcctcgacgg
     2461 acgcgacgct gaagatggat ctctcgaagc tccagcacga cttctcgaaa taggtcctga
     2521 gcgcgcccgg cgccgcgggg cgtctcgggc gcgcgcgagg ggcgtcgggt ccccgggacc
     2581 cgacaccccg tcttccccgc agttcatccc ggcagccccc tggctgttcc cgcacaaagg
     2641 agcgctccat gaaagtcggc atcgacagct attcctatca ccgctacttc ggcgaggtgt
     2701 acgacgtcca gaaaaagccc gcaaaggaca tgagctacga agacttcctg aagagggcgg
     2761 tccagctcaa ggtcgacggc gtttcgctgg agacatgctt tttccagagc accgacgagt
     2821 cctacctgaa aagactgaag gaaatcatcg accgcggccc gctggaagtg gtggtggcct
     2881 ggggccaccc caagggcttc gaaggcggga agaagccgga ggagctggcc gacctgcgac
     2941 ggcatttcac cacctgccgg atcctcggtg cgaaggtgat gcgggtggtc ggctcctcgc
     3001 tggactaccg aaacgacccc cacggccccc agatcgaaaa gctgaccggg atattcaagg
     3061 aatgcgcgaa gatcgccggc gatgaagggg tgaagctcgc ggacgagaac cactacgact
     3121 tcaccatgga cgagtacctc ggcctgttcg aggcggtcgg ctcgccgcac ttcggcatgt
     3181 gcttcgacac cggcaactgc ctccgcaacg gcgacgagcc ggtggagtcg gcgcgccggc
     3241 tgggcaggta catcttcgcc acccacacca aggatgtcat gccgctctat ggtggaaatc
     3301 cgaaagattg gttctactat gcatgcacgc cggtggggaa gggactggtc aatttccccg
     3361 gcatcgtcgc ggagctggag aagcagggct acgccgggct gtacgcggta gagatcgact
     3421 acccgtaccc gtcgatccgt gacgaggacc aggcggtgaa ggagagtatc tcctacctga
     3481 agacgctgca gaagagccat ccgaagagat agggggacgg tcatgaaagg cttccggaag
     3541 atcgactacg ggaagtccgg cggcgcgcgc cggttcggta tcggggtcat c
//
LOCUS       DASRZS010000085         3238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_430587, whole genome shotgun sequence.
ACCESSION   DASRZS010000085 DASRZS010000000
VERSION     DASRZS010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430587"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1798
                     /locus_tag="VFH83_02095"
     CDS             <1..1798
                     /locus_tag="VFH83_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HET6485179.1"
                     /translation="RYEPLPNGDSAKSAIKQVASARFGVTSHYLVNSRELQIKMAQGA
                     KPGEGGQLAGLKVDGIIAKVRHATPGVTLISPPPHHDIYSIEDLAQLIYDLKSANPEA
                     RVSVKLVSEVGVGTIAAGVAKGRADMVLICGYDGGTGNSPISSIKHAGIPWEIGLAET
                     QQTLVMNRLRDRIRVQTDGQLRTGRDVAIAALLGAEEFGFGTISLVTLGCVMMRKCHL
                     NTCPVGVATQDPRLRARFQGKPEHVIAFMRFIAQDTREVMASLGFRTIEEMVGHVEVL
                     ETRAAVDHWKAKGLDFSRVLAKPEVPAGTSLRCTRGQQHDFSLSLDRELIERARPALE
                     GREKVRLFLSIRNCNRTVGATLSAQISKCHGAKGLPADSITVSFTGSAGQSFGGFLAP
                     GITFELEGDANDYFAKGLSGGKIIVYPPRAATFRPQDNIITGNVNLFGATSGEVYING
                     MAGERFAVRNSGAIAVVEGVGDHACEYMTGGTVVVLGRTGVNFAAGMSGGVAYVLDED
                     QLFDTKCNLEMVDIEPVSDARDQRTLRQIVERHVMYTRSEYATTILRDWGEMLPRFVR
                     VMPIDYKRALERIAKDQAKETDVVRMTEEVYH"
     gene            1798..3222
                     /locus_tag="VFH83_02100"
     CDS             1798..3222
                     /locus_tag="VFH83_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HET6485180.1"
                     /translation="MGHVSGFIDFQRKSYTYLPVKERLQNYKEFTVQQPEEELAKQGA
                     RCMDCGVPYCHAMGCPVYNLIPEWNDLVYRGDWKEALVRLEMTNNLPEMTGRVCPAPC
                     ETSCTLSINSSPVTIKQVELAIVERGFAEGWVIPRPPTTLTGKRVAVVGSGPAGLSAA
                     QQLRRAGHEVTVFERSPRVGGLLRYGIPDFKLEKRVIDRRLAQMEAEGVRFQTNVTIG
                     EDISARYLRANFDVILLAMGAGEPRDLPVPGRDLGGVHFALDYLTLSNQLQNGEIDAS
                     RIISAKDKVVLVIGGGDTGSDCVGTANRQGATKVHQFEILPKPPEWKESTNPSWPLWP
                     NILRTSSSHEEGCERDWSITTQRFLGEDGSVQGGTFNRVEWKRQPNGQMKMVDAPGTE
                     FSLKVDLVLLAMGFVHVRHGKILEELGVEYDPRGNIRCNPDYSTSAPGVFAAGDAHTG
                     ASLVVRAIYHGRESAKAVDRYLRG"
BASE COUNT          583 a         1063 c         1093 g          499 t
ORIGIN      
        1 ccgctacgag ccgctgccga acggagacag cgccaagagc gccatcaagc aggttgcttc
       61 cgcccgattc ggcgtgacgt cacattacct ggtgaactcc cgggagctcc agatcaagat
      121 ggctcagggc gcgaagcccg gggaaggcgg tcagctcgcc gggctcaagg tggacggcat
      181 catcgccaag gtccgccacg ccacgccggg cgtcacgctg atctctcccc ctcctcacca
      241 cgacatctat tccatcgaag acctggcgca gctcatctac gatctgaaga gcgccaaccc
      301 cgaggcgcgc gtttcggtca agctggtctc cgaggtgggc gtgggaacca tcgcggctgg
      361 cgtggccaag ggtcgggccg acatggtcct catctgcggc tacgacggcg ggacgggaaa
      421 ctccccgatc tcgtcgatca agcacgcggg catcccctgg gagatcgggt tggccgagac
      481 gcagcagacg ctggtcatga accggctgcg ggaccgcatc cgggtccaga cggacggaca
      541 gcttcggacg ggccgggacg tggccatcgc ggctctgctg ggggcggagg agttcgggtt
      601 cgggaccatt tctttggtta cgctgggctg cgtcatgatg cgcaagtgcc acctcaacac
      661 ctgccccgtc ggcgtggcca cgcaagaccc gcggcttcgg gccaggttcc agggcaagcc
      721 ggagcacgtc atcgccttca tgcggttcat cgcccaggac acgcgggagg tcatggccag
      781 cctgggcttc cgcaccatcg aggagatggt ggggcacgtc gaggtcctgg aaacccgggc
      841 cgcggtggac cactggaagg ccaagggcct ggacttctcc cgggtcctgg caaagcccga
      901 ggttccggcc ggtacctcgc tgcgctgcac gcgcggccag cagcacgatt tctcactctc
      961 cctggaccgg gaactgatcg agcgggccag gccggccctg gaaggccgtg agaaggtccg
     1021 cctcttcctg tccatccgca actgcaaccg gaccgtcggg gctacgctct ccgcgcagat
     1081 ctccaagtgc cacggcgcca agggcctacc ggcggacagc atcacggtga gcttcacggg
     1141 ttccgcgggc cagagcttcg gcgggttcct ggctcccggg atcaccttcg agctggaagg
     1201 ggacgcgaac gactatttcg ccaaggggct ctccggcggc aagatcatcg tctacccgcc
     1261 gcgggccgcc acgttccgac cccaggacaa catcatcacg ggaaacgtca acctcttcgg
     1321 cgccacctct ggcgaggtct acatcaacgg gatggctggc gagcgcttcg cggtccgaaa
     1381 cagcggcgcc atcgccgtgg tggagggcgt gggagaccac gcctgtgagt acatgaccgg
     1441 cggcaccgtc gtggtgctgg gccggaccgg cgtcaacttc gcggccggca tgagcggcgg
     1501 ggtggcctac gtgctggatg aggaccagct tttcgacacg aagtgcaacc tggagatggt
     1561 ggacatcgag ccggtgagcg acgctcggga ccagcggacg ctgcgccaga tcgtggagcg
     1621 ccacgtgatg tacacccgaa gcgagtatgc cacgaccatc ctgcgggact ggggagagat
     1681 gctgccccgg ttcgtccgcg tgatgcccat cgactacaag cgcgcgctgg agcgcatcgc
     1741 caaggaccag gcaaaggaga cggatgtggt ccgcatgacc gaggaggttt atcactagtg
     1801 ggccatgtca gcggctttat cgactttcag cgcaagtcct atacctacct ccccgtaaag
     1861 gagcggcttc agaactacaa ggagttcacg gtacagcagc cggaggagga actggcgaag
     1921 cagggtgcgc ggtgcatgga ctgcggcgtt ccctactgtc acgccatggg ctgccccgtg
     1981 tacaacctca tccccgagtg gaacgacctg gtctaccggg gggactggaa agaggccctg
     2041 gtgcggctgg agatgacgaa caacctgccg gagatgaccg gcagggtctg tcccgctccc
     2101 tgtgagacct cctgcaccct ctccatcaac tccagcccgg tcaccatcaa gcaggtcgag
     2161 ctcgccatcg tcgagcgcgg gttcgcggag ggatgggtca tcccccggcc gccaaccacg
     2221 ctcaccggca agcgcgtggc cgtagtgggc agcggtcccg cgggactgtc cgctgcccag
     2281 cagcttcgcc gcgcgggcca cgaggtcacg gtcttcgagc gctccccgcg cgtcggcggc
     2341 ctcctgcgct acggcatccc ggacttcaag ctggagaagc gcgtcatcga ccgtcgcctg
     2401 gcccagatgg aggcggaagg ggtccggttc cagaccaacg tcaccattgg ggaggatatc
     2461 tccgcgcggt acctgcgcgc caacttcgac gtgatcctcc tggccatggg ggcgggagag
     2521 cccagggacc tgccggtccc cggacgagac ctcggcgggg tccacttcgc cctggactac
     2581 ctcacgctgt ccaatcagct ccagaacggg gagatcgacg catcgaggat catcagcgcc
     2641 aaggacaagg tggtcctggt gatcggcggc ggggacaccg gctcggactg cgtcggcacc
     2701 gccaaccgcc agggagccac caaggtccac cagttcgaga tcctgcccaa gccaccggag
     2761 tggaaggaga gcaccaaccc aagctggccg ctctggccca acatcctgcg gacctccagc
     2821 tcccacgaag agggctgcga gcgggactgg tccatcacca cgcagcggtt tctaggagag
     2881 gacgggtcgg tccagggtgg gaccttcaac cgcgtggaat ggaagcgcca gccgaacggg
     2941 cagatgaaga tggtggacgc gccggggacc gagttctccc tgaaggtgga cctcgtcctc
     3001 ctggcgatgg ggttcgtgca cgtgcgccac ggcaagatcc tggaggagct tggggtggaa
     3061 tatgacccgc gcgggaacat ccgctgcaac cccgactact ccacctcggc gccgggcgtg
     3121 tttgcggccg gggacgcgca cacgggcgct tccctggttg tgcgcgccat ctaccacggc
     3181 cgcgagtcgg ccaaagccgt agaccggtac ctgaggggct gagggcagcg cggtccct
//
LOCUS       DASRZS010000086         3717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3125498, whole genome shotgun sequence.
ACCESSION   DASRZS010000086 DASRZS010000000
VERSION     DASRZS010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3125498"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..764
                     /locus_tag="VFH83_02105"
     CDS             282..764
                     /locus_tag="VFH83_02105"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET6485181.1"
                     /translation="MGGGRWGRFFAAGELRLALLSLLEDGPKHGYELMKTLESKTGGT
                     YRASAGAVYPTLQQMEDEGLVTSEQRDGKRVYQITEAGRAECAQKAEAIKELWERASG
                     SDDWRDFFGPHVASMAGPAAHVMKAAVRAAARSRDDPDKTARIFTILDQARRDLDAIP
                     "
     gene            872..1375
                     /locus_tag="VFH83_02110"
     CDS             872..1375
                     /locus_tag="VFH83_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6485182.1"
                     /translation="MSGGTTRGQLFLAELDAEVASSRNCLLRVPENLFDWKPHPKSMP
                     LGYLSLLVAEIPLWIALTVEQGEIDFATYKRFQPKSTAELVSHLDECVQRARRALQNA
                     TDEALAKPFRLKRGDKVLMSSPIQESVMQSINHWVHHRGQLTVYLRLNDIPVPSIYGP
                     SADEGTF"
     gene            1417..2418
                     /locus_tag="VFH83_02115"
     CDS             1417..2418
                     /locus_tag="VFH83_02115"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6485183.1"
                     /translation="MRASAVVCSLVAVGLLLISCSPPTRAPEGGAEAAVREYPPDRPY
                     PGSDFIPVDGYRIHLLRWMPAADVSVRGRVLLVHGFGASVYGWRLLGPALAERGYLTV
                     AADYPPFGYSTPSEAEREGTVAGTPGNRARLLWDLVDSEDDRLGATGSDGWILIGHSL
                     GGRIAAWMAGTRPSRMAGLVLIAPAILGDVSGPMAGPILQGWLRANLDPTLKDGARIR
                     SILAQAYGRNPTDEETRGYWAPLLRPHEAESLLAWSRHAAEPVGPPLSAIVTPTLILW
                     GTADRVVKPEGARLAGQIRASRFVDIPGGSHCLMETQFDPTWAAVSEFLDRLQAEGH"
     gene            complement(2415..3293)
                     /locus_tag="VFH83_02120"
     CDS             complement(2415..3293)
                     /locus_tag="VFH83_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6485184.1"
                     /translation="MLTEAQVRQFQRDGFLNAGVMVTETELGELSRDLDDAIRRGPDG
                     FPEGSPRPVLFRDLYASGGDYTAKAAVSVQPVWQIVNIWEAFPSFRRLIHHPSIVAAI
                     SQLTGFTDLQVWHDQVQYKPPRAGGSTHWHQDAPLWPAIEPMTPVSAWIPFDDADVSN
                     GCMWMVPGSHRWGNQRAYLDTLQHLNTAEEFTRFLPFAPPQDAEIGVIQARPCPVRRG
                     EVHFHHSMTWHGSPKNPSERPRRAIAIHYMSSEARYTGKDHAMSRFISVRPGDAMANA
                     GPHFPRVMRSGKMVPA"
     gene            3388..>3717
                     /locus_tag="VFH83_02125"
     CDS             3388..>3717
                     /locus_tag="VFH83_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485185.1"
                     /translation="MKSQTLDLSPATLETYGREGFLVCESVIDRSEVEEAREAISMII
                     RSVHASLLRGSPDVEIQPTPADARANYAGLEVKRVGSEFRLRFEAGTDPRRMGPEEAE
                     KSVRVLYS"
BASE COUNT          619 a         1214 c         1245 g          639 t
ORIGIN      
        1 atgattctat cccgttctcg atccaagttc aggagaattt ggagaaaata gcctggcaga
       61 acggtagagc actgtgggcc aagttgccca gaacgactca tgagaaccgg ctccacatgg
      121 gaagccacgc cacaacagca aaccactgct gcccttctat agtgttactt tagactttac
      181 tttgagtata tctatagcta tctttagata gtactttagg aggtacacca atgcacagag
      241 gttggggaca ctgggggggt ccttggagcg gttggcaggg aatgggaggc ggcaggtggg
      301 gcaggttctt tgctgcaggc gagctcaggc ttgcgctgct gtctctcctg gaggacgggc
      361 caaagcatgg ctacgagctc atgaagaccc tggagtcaaa aaccggcggg acctaccgcg
      421 cgagtgccgg ggcggtctac ccgacgctcc agcagatgga ggacgagggg ctggtcactt
      481 cagagcagag agacggcaag cgggtctacc agatcaccga ggccgggaga gccgagtgcg
      541 cccagaaagc ggaagccatc aaggagctct gggaacgggc aagcggctcc gacgactggc
      601 gggacttctt cggtcctcac gtggccagca tggctggacc ggcggcgcac gtaatgaagg
      661 ccgcggtccg agcggcagcg cggtcccggg acgatccgga caagactgcg aggatcttca
      721 cgatcctgga ccaggcccga cgggacctcg acgccatccc ctgaggccta cggcgcttcc
      781 cttggcgcta cggcgcttcc cttggcgcta ttgcgcttcc cttggcgcta cggcgcttcc
      841 cttgagcgca ccccgtgagt agcttccccg tatgagcggc ggaacgactc gcggtcagct
      901 tttcctcgcg gagctggatg cggaggtggc ttcctctcgg aactgcctcc tccgcgtccc
      961 ggagaacctc ttcgactgga agcctcatcc caagtccatg cctctcggct acctgtccct
     1021 gctggttgcg gagataccgc tctggatcgc gctcaccgtc gaacaaggcg agatcgactt
     1081 cgcgacatac aagcgcttcc agcccaagag caccgctgag ctggtaagcc acctggacga
     1141 gtgcgtccag cgagcgcgcc gagcgcttca gaacgccacc gacgaggccc ttgccaagcc
     1201 tttccgcctg aagcgcggcg acaaggtgct catgagctct cccatccagg aaagtgtgat
     1261 gcagtccatc aaccactggg tccaccatcg gggtcagctc accgtctacc tgcggttgaa
     1321 cgacatcccc gtgcccagca tctacgggcc gtccgccgac gaggggacct tctagccgcc
     1381 tgcccccggc cgacccgcat cggatatact ggacccatgc gcgcatccgc ggtcgtgtgc
     1441 tcgctggtgg cagtgggcct gctgctgatc tcctgctccc cgcccacgcg agcgcccgag
     1501 ggcggcgccg aagcggcggt ccgggagtat ccgccggatc ggccgtaccc aggctccgac
     1561 ttcattcccg tcgatggcta ccggatccac ctgctgcgct ggatgccggc cgccgatgtc
     1621 tccgtccgcg gccgggtgct cctggtgcac ggcttcggcg cctccgtgta cggctggcgc
     1681 ctgctcgggc ccgcactcgc cgagcgcggc tacctcaccg tggccgccga ctacccgccg
     1741 ttcggctact ccactccctc cgaggcggaa cgggagggca cggtggcagg aacccctggc
     1801 aaccgggccc gtctcctgtg ggatctcgtg gattcggagg atgatcgtct gggagcgacc
     1861 ggcagcgatg gctggatcct gatcggccat tcgctggggg gccgtatcgc ggcctggatg
     1921 gcggggacgc ggccgtcccg gatggccggg cttgtcctta tcgccccggc aatcttgggt
     1981 gatgtatcgg gccccatggc cgggccgatc ctgcagggat ggcttcgcgc caacctggat
     2041 ccgacgctga aggacggcgc caggatccgg tccatccttg ctcaggccta tggccggaat
     2101 ccgaccgatg aggagacccg tggttactgg gcgccgcttc tgaggccgca cgaggctgag
     2161 agcctgctgg cctggtcccg gcacgccgcc gagccggtgg ggccgccgct ttccgcgatc
     2221 gtcaccccaa cgctgatcct ctggggaacc gcggaccgcg tggtcaagcc ggaaggggcc
     2281 cgcctggccg gccagatccg cgcctctcga ttcgtcgaca ttccgggcgg gtcccattgc
     2341 ctgatggaga cccagttcga cccgacctgg gcggccgtgt cggagttcct ggatcgcctc
     2401 caggcggaag ggcactaggc cgggaccatc ttgccggagc gcatcacgcg cgggaagtga
     2461 gggccggcgt tggccatggc gtcccccggc cgaacggaga tgaaccggct catggcgtgg
     2521 tccttgccgg tatagcgtgc ctcgctggac atgtagtgga tggcgatggc ccgtctcggc
     2581 cgctccgatg ggttctttgg agatccgtgc caggtcatgg agtggtggaa gtgcacctcc
     2641 ccgcgcctca cggggcaggg gcgcgcctgg atcacgccga tctccgcgtc ctggggcggc
     2701 gcgaacggca ggaaccgggt gaactcctcc gccgtgttca ggtgctggag cgtgtccagg
     2761 tacgcgcgct ggtttcccca ccggtgcgag ccgggaacca tccacatgca gccgttcgac
     2821 acgtccgcgt cgtcgaacgg gatccaggcg ctcaccggcg tcatgggctc gatggcgggc
     2881 cacaggggag cgtcctggtg ccagtgggtg gagccgcccg cccggggagg cttgtactgc
     2941 acctggtcgt gccagacttg caggtccgtg aaaccagtga gctggctgat ggcggccacg
     3001 atcgacgggt gatggatgag ccggcgaaag gaggggaacg cctcccagat gttgacgatc
     3061 tgccacaccg gctgcaccga caccgcggcc ttggcggtgt agtccccgcc gctcgcgtac
     3121 aggtccctga agagcaccgg ccgcggggag ccttcgggga acccgtccgg cccccgccga
     3181 atcgcgtcat cgagatcccg agagagctcg ccgagctcgg tctccgtcac catcacccca
     3241 gcgttcagga atccatcccg ctggaactgc cgcacctgcg cttccgtgag catggaaggg
     3301 aatatactga cgctccgcgt cgggctccaa acgacggctc cttgaccggt ccccctccgc
     3361 gcgcgacctt cagggattgg ggaacgtatg aagagccaga ctctggacct gagtcccgcc
     3421 acgctggaga cctatggccg ggaaggcttt ctggtgtgtg agtccgtgat cgaccgttcc
     3481 gaggtcgagg aggccaggga agcaataagc atgatcatcc gctccgtgca cgcctccctg
     3541 cttcgcggct cgccggacgt ggagatccag cccacgccgg cggatgcccg ggcaaactat
     3601 gccggcctcg aggtcaagcg cgtggggagc gagtttcggc tccggttcga ggcgggaacg
     3661 gatccccgaa ggatggggcc ggaggaggcc gagaagagcg tgcgcgtcct gtactcc
//
LOCUS       DASRZS010000087         3448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_816189, whole genome shotgun sequence.
ACCESSION   DASRZS010000087 DASRZS010000000
VERSION     DASRZS010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816189"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VFH83_02130"
     CDS             <1..1027
                     /locus_tag="VFH83_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004491673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase"
                     /protein_id="HET6485186.1"
                     /translation="GFTQQEIEEAERWACGTMTLEGAPHLSPDHYPVFDTANPNGKIG
                     KRSIAWRAHIEMMAAVQPFVSGAISKTINMPSNASVDDIKGAYLLSWRKMLKAMALYR
                     DGSKLSQPLSSLMPESDAAASAVLTVMNDVKQEGIGLAVSPSQVAKKRNPLPAKRFGY
                     TQKAKIGGHSIFLRTGQYQDGSLGEIFLDMHKEGAAFRSLLNSFAIAVSLGLQYGVPL
                     EEYVDAFTFTRFEPNGMVSGHDHIKMTTSVLDFIFRDLALSYLGRSDLVQVKPDDLIS
                     TAMNRNGTTPTSAEASESLPLDNEVHVARMKGYEGDPCPVCGHFTLVRSGTCMRCDTC
                     GSTTGCS"
     gene            1138..2403
                     /locus_tag="VFH83_02135"
     CDS             1138..2403
                     /locus_tag="VFH83_02135"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6485187.1"
                     /translation="MSEPGSSALGFQKPRLVNRLFYRVMTSLLRGVILRYFRLSPVDA
                     QVVPDVGAGILLSNHSTLFDPIWVYAMLRRPVYFAATEDLFRKRALGRLIRWFGAFPK
                     RKAASDIAALRAIFTLIGTGGLIGVFPEGVRTWDGRNQPLYTGIAKLIQRMAVPVYVC
                     RLEGAYLVYPRWARRWRRLPIRGVFSRLYDSADIPADENRILEDIAEAIRTRDYELPI
                     EPSENRGSGLAVNLTRVLYRCPSCGTLEGLKLVRPYSSNLVECSSCFSSWSVDIGCRL
                     STVDENGRPEGRWTTLADYYDRVRSMPLTPIRSELRLGLAPGERIFLISRPRFLFKQE
                     RFPNLRVFAFGRAFLTSERLLFRTRLGFPLAMPLTSVGALSVDPGDRLHFTYEGKLYR
                     IPFRNESALKWYDTILRQQAEIRARKAVG"
     gene            2454..3026
                     /locus_tag="VFH83_02140"
     CDS             2454..3026
                     /locus_tag="VFH83_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET6485188.1"
                     /translation="MRSRSSVRRYDERSIPEEDVRSIVEAARLAPSAENSQPSRFIAV
                     TSRPALERVARLCFSGIFRPTRFAAKAPLIIALCAERGSLVEAGKSIKDRAMYQLDCG
                     IAGEHLVLRAAELGLGTCWIGWFNRAGARKALGVPFHVRVVALISVGYPAADLPPRPK
                     VRKPLDQILYRDAWGQSFPGAGAVQDDVEK"
     gene            complement(3005..>3448)
                     /locus_tag="VFH83_02145"
     CDS             complement(3005..>3448)
                     /locus_tag="VFH83_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485189.1"
                     /translation="VLDYELPEGVLDVRYRIVVDGLWTVDPTNAQSDTDALGTSISVF
                     TLAQEPPRPIVNPRPHPDGSLTFTFRGTPGKRVALVGDFNNWDPFVDYLSETSPGLFT
                     ITLRVAPGPHWYCYFTEGRRVLDRYNGETGSDPDGTRTSYFSTSS"
BASE COUNT          555 a         1162 c         1141 g          590 t
ORIGIN      
        1 gggcttcacc cagcaggaga tcgaagaggc ggagcggtgg gcgtgcggga ccatgacgct
       61 ggaaggcgcg cctcatctct ctccggatca ctacccggtg ttcgacacgg ccaaccccaa
      121 cgggaagatc ggcaagcggt ccatcgcctg gagggcccac atcgagatga tggccgccgt
      181 gcagcccttc gtgagcggag ccatctccaa gaccatcaac atgccgtcca acgcctccgt
      241 ggacgacatc aaaggggcgt acctgctctc gtggcgcaag atgctgaagg ccatggcgct
      301 ctaccgcgat ggctccaagc tcagccagcc gctgtcttcc ctgatgccgg agtccgatgc
      361 cgccgcgtcc gccgtcctca ccgtcatgaa cgacgtgaag caggagggga tcggcctggc
      421 cgtctcaccg tctcaggtgg ccaagaagcg aaacccgctg ccggccaagc gcttcgggta
      481 tacccagaag gcgaagatcg gaggccattc catcttcctt cgcacggggc agtaccagga
      541 cggatccctg ggcgagatct tcctggacat gcacaaggag ggggcggcgt ttcgatccct
      601 cctcaacagc ttcgccatcg ccgtgtcgtt gggtctgcaa tacggcgtgc cgctggagga
      661 gtacgtggac gcgttcacct tcacccggtt cgagcccaac gggatggtga gcgggcacga
      721 ccacatcaag atgaccacgt cggttctgga cttcatcttc cgcgacctgg cgctctccta
      781 cctgggccgg tccgacctgg tgcaggtgaa gccggacgac ctgatctcca cggccatgaa
      841 ccgcaacggc accaccccga catccgcgga agccagcgag tccctgcccc tggacaacga
      901 ggtccacgtg gcccggatga agggctacga gggggacccc tgtccggtgt gcgggcactt
      961 cacgctggtc cgaagcggca cctgcatgcg gtgcgacacc tgcggctcca ccacggggtg
     1021 tagctgaaag gcggtccctg gggcggcccg cgttcaccgg ggccccgctc cacccgggcg
     1081 ccccggacaa gctcgccgcc ttgatgcggg gcgccggttt tgggtatcct tcgcgcgatg
     1141 tctgagccgg gcagctccgc gcttggcttt cagaagccac ggctggtgaa tcgcctcttc
     1201 taccgagtaa tgacctccct cctgcggggg gtgatcctgc ggtatttccg gctgtctccg
     1261 gtggacgcac aggtggtccc ggatgtgggc gccggcatcc tcctgtccaa ccactccacg
     1321 ctcttcgatc ccatctgggt ctacgcgatg cttcgccgtc ccgtatactt cgccgccacg
     1381 gaggatctgt tccgcaaacg ggccctgggg aggctgatcc gctggttcgg cgcgttcccc
     1441 aagcggaagg cggcatcgga catcgccgcg ctgcgggcca tcttcaccct catcgggacc
     1501 ggagggctga tcggcgtgtt tcccgagggc gtccgcacgt gggacggcag gaaccagccc
     1561 ctctataccg gaatcgccaa gctcatccag aggatggccg tgccggtcta cgtgtgtcgg
     1621 ctcgaaggcg cgtacctggt ctatccgcgg tgggcccgac gctggaggag actccccatc
     1681 cgcggcgtgt tctcgaggct ctacgattcc gccgatattc cggcggacga gaatcggatc
     1741 ctcgaggaca tcgcggaggc catccgcacg cgcgactacg agcttcccat cgagccgtcg
     1801 gaaaaccgcg ggtccggcct tgccgtcaac ctgacgcgcg tgctctatcg gtgcccgagc
     1861 tgcggcaccc tggaaggcct caagctggtg cggccgtact cctccaacct cgtcgagtgc
     1921 tcgagctgtt tctcttcctg gagcgtggac atcggctgcc ggctctccac cgtggatgag
     1981 aacgggcgac ccgaggggcg ctggaccacc ctggccgact actacgaccg ggtccggtcg
     2041 atgcccctca cgccgatccg aagcgagctc cgcctggggc tggcgccggg ggagcgcatc
     2101 ttcctgatct cccggcctcg cttcctcttc aagcaggagc ggttcccgaa cctgcgggtc
     2161 ttcgcgttcg gcagggcgtt ccttaccagc gagcggctcc tgttccgcac ccgcctcggc
     2221 ttcccgctgg ccatgccgct cacctcggtg ggcgcgctgt ccgtggaccc gggcgatcgg
     2281 ctgcacttca cctacgaggg caagctctac cgcatcccct tccgcaacga gagcgcgctc
     2341 aagtggtatg acaccatcct gcggcagcag gccgagatcc gggcgcgaaa ggccgtcggg
     2401 tgagcacccc gccgccggga gtccggcgaa atccgtcgcc ccttttgtcc atcgtgcgaa
     2461 gccggtccag cgtgcgccgc tacgacgagc ggtccatccc cgaggaggac gtgcggtcca
     2521 tcgtggaagc ggcccgtctg gccccttccg ccgagaactc ccagccctcg aggttcatcg
     2581 cggtgacgag ccggccggcg ctggagaggg tggcgcgtct atgcttctcg gggatcttcc
     2641 gcccgacccg gttcgccgcc aaggcgccgc tcatcatcgc cctgtgcgcg gagcgcggca
     2701 gcctggtgga agccggcaag tccatcaagg accgggcgat gtaccagctc gactgcggga
     2761 tcgcgggaga gcacctggtg ctgcgggcgg cggagctggg actgggaacc tgctggatcg
     2821 ggtggttcaa ccgggcgggc gctcggaagg cgctgggagt gccgtttcac gtgcgggtgg
     2881 tcgcgctgat ctcggtggga tacccggcgg cggaccttcc accgcggccg aaggtgagaa
     2941 agcccctgga tcagatcctg taccgggacg cctggggcca gtcgttcccc ggtgccgggg
     3001 ccgttcagga cgacgtggag aagtagctgg tcctggtgcc gtccggatcc gagccggttt
     3061 ccccgttgta ccggtccagg acgcgcctgc cctcggtgaa atagcagtac caatgcgggc
     3121 ccggcgccac gcgcaaggtg atcgtgaaga gcccgggcga tgtctcagac aggtagtcca
     3181 cgaacggatc ccagttgttg aagtctccga caagcgcgac gcgcttgccc ggagtccctc
     3241 ggaacgtgaa ggtgagggaa ccatcgggat gaggcctggg attgacgatg ggtctcggcg
     3301 gctcctgcgc cagggtgaac acggagatcg acgttccgag cgcatccgtg tcggattgag
     3361 cgttcgtagg gtccaccgtc cagagcccat ccacgacgat ccgatagcgg acgtccagga
     3421 cgccctcggg cagctcgtag tccagaac
//
LOCUS       DASRZS010000088         6097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774 k141_46185,
            whole genome shotgun sequence.
ACCESSION   DASRZS010000088 DASRZS010000000
VERSION     DASRZS010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46185"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1274)
                     /locus_tag="VFH83_02150"
     CDS             complement(141..1274)
                     /locus_tag="VFH83_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485190.1"
                     /translation="MSVEVSTVGSRRELKAFVTFPMRLYSGNPYWVPPLIRDEMETFT
                     REKNPAFDEAEARLFLARRDGAVVGRVAAILSHAANRKYNTRNLRFGWFDTIEDFEVA
                     RALLAAVEAWGKELGMQTITGPHGFTDLDPEGLLVEGFGELATISVIYNHPYYPGLLE
                     RCGLAKDVDYVEFQASAPQGTTIPEKMVKMAEWAAKRNGYRLLHYPKISTLRKERAAE
                     LFDLVDEAFEELYGTVPLTPRQKNYYINKYIPFANPDFIEIVVNEQGVMIGFLIAIPS
                     LARAFQKARGRLFPLGFLHILTALRRFDTLDFLLAGVKKEYRGKGVDLIMTIDVFRTG
                     LARGVRKAESNPELELNSKIQNEWKIVSTRQHKRRRIYRKSIA"
     gene            complement(1321..3603)
                     /locus_tag="VFH83_02155"
     CDS             complement(1321..3603)
                     /locus_tag="VFH83_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET6485191.1"
                     /translation="MVVTLTAVATLVLAGFAVRVQINPDFTSLLPQDDKVNQILKEYA
                     GTTEALPDLLVLAATASAPGGDIFAPDQLSGFFQAVGSIAAMPGVQSAMTPFNLVSFD
                     RVNGRLLLQPMAGGDSIPQDIPAFRARLLSTGYARNLVVSSDGTMLIAFFPCEHRDSF
                     KPFMDRVDQVAAGMRARGLTPFLTGTIPLSVRTGMHLASDASRLLALAALIILLSYVV
                     GFRSFRAIALPLLSVILGTVWTVGFMGVMGYPLSLITIVAPPLILIFGNEYNIFTTNE
                     YLRVAGVEGTAPGWIVRASRAVAKPIAMAFLTTVVGFLSLCIGSIRQTRQFALTASFG
                     SLACAFLALFFLPALFALLKDPVVRTHQPDGAFTRLMRSLARFANRYPAIILGLLSAV
                     VILFATTLHLLVFNTDPASYFPQSDPVLQDMQAIYRSAGGYEQVSVSFDAPGRAAGYF
                     QDPAILKKVQGVETALRGIHDISYALSLPDLLQELNRAATGRDELPANRAIITVFSRV
                     ITASGGSSPGAAVLSNLVNKDFSRLTLSFRIYNSTTAHYMDEARFRSLLASMNQVLAA
                     NPIGATPVVWGDLLRNLAFADSLRRTLLLSMALSIVCILALTIWVFRSVRHGLYPIVP
                     LVTGLLLNYSMMALTRIPLDMTTIMVSNIAIGVGVDSAIYLVIQFRRDLARSPGDPGL
                     ALERTLGAIGLPILLSSASIIVGLLVFATASFRPVVYFGMLVLFTLLATTAGTLVTLP
                     ALLAMDTRARLRRAQREARP"
     gene            3768..4508
                     /locus_tag="VFH83_02160"
     CDS             3768..4508
                     /locus_tag="VFH83_02160"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6485192.1"
                     /translation="MKTRIRPALVLLLSALTVPLAAQNTGQTVSVRSGGKDYVSYLAA
                     GPGAEQKPGIVLVHSFNGLEQGYKDMVDRFASDGFVVLAVGWQTFEQSPSDATVERLV
                     RDGVAMLKARSDVDPSRIGLTGFCAGGRYTMLLLPVIDDFAAGVAWYGFPYSGGSAAQ
                     PVRPVDEIDRLSHPLLMIHGSADQASPITNIFNYATALQKASKYFELKVYEGQPHGFM
                     VQNGQLRKDSIGLDAYQEMVTFFHRNLG"
     gene            4510..4836
                     /locus_tag="VFH83_02165"
     CDS             4510..4836
                     /locus_tag="VFH83_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485193.1"
                     /translation="MTDWAAAVSTRLGRTDPARLQNTLMAMSDKICAVALATLPEERR
                     DAVYALIAPTKARRIREEIRLETRRRTARAVRDRIIREFLKSFDAGPKTRPTIYIRPK
                     RDSRLR"
     gene            complement(4851..5522)
                     /locus_tag="VFH83_02170"
     CDS             complement(4851..5522)
                     /locus_tag="VFH83_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6485194.1"
                     /translation="MSAAEAPSLTAELLGRTWLSPRVFQLELARPPGLSFAAGQRVRI
                     HRGPVERDYTLACGPAAPTLRLLVRLVEQGVVSGLLAETRLGASLVLSGPHGYFVFRD
                     ASVASVFVATGTGVAPFVAMAASGVCPSLLLHGVKRPEDLHYRVLLSKAAARYVPCLT
                     EGPHRPEDPDPAYPGRVVAWLRDRLPAGVYDFYLCGNAAMIRDVTLLVDERFPGSRVF
                     AEGFH"
     gene            complement(5519..>6097)
                     /locus_tag="VFH83_02175"
     CDS             complement(5519..>6097)
                     /locus_tag="VFH83_02175"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET6485195.1"
                     /translation="GDKGMTSLFSGERVSKNHDRIEACGALDELGSVLGALASALSAA
                     VLPSAEAATTEGASRLGSEIQRVQGELLSAGAWLATTPGSASRGMLRPFGPEPTGHLE
                     AAVERIQAALPPLEHFILAGGHPVASWAHVARAVCRRAERQALRLAVPASGPDPELAN
                     ILAYLNRLSFYLFVAARYCNQLFGTPERTWQG"
BASE COUNT         1134 a         1923 c         2113 g          927 t
ORIGIN      
        1 agggattcct cgagtcggga gggatcgatg ggggagtcca gttccagcgt caggcgaacc
       61 gtacggtcat gaccaagggc gatcccgacg gcgaaccagt cctgcacggt ggcaggacga
      121 cgaaacagcc ccgtcatggg ctacgcgatg ctcttgcgat agatccgccg ccgcttgtgc
      181 tggcgcgtgg acacgatctt ccactcgttc tggatcttcg aattgagctc gagctcggga
      241 ttggactcgg cctttcgaac gccccgcgcc agtcccgttc ggaacacgtc gatggtcatg
      301 atgagatcca cccccttgcc ccggtattcc ttcttcacgc cggccagcag aaagtcgagg
      361 gtgtcgaatc gccgcagagc cgtcaggatg tgaaggaagc ccagggggaa cagccttccc
      421 cgtgccttct ggaaggcgcg ggccaggctg ggtatcgcga tcaggaaccc gatcatcacc
      481 ccctgctcat tcaccacgat ctcgatgaaa tcggggttgg cgaaggggat gtacttgttg
      541 atgtagtagt tcttctggcg cggcgtgagc ggcacggtcc cgtagagctc ctcgaacgcc
      601 tcatccacca ggtcgaagag ctcggcggcc cgttccttgc gcagcgtgga gatcttggga
      661 tagtggagga gccggtaccc gttgcgcttg gccgcccact ccgccatctt gaccatcttc
      721 tccgggattg tggtgccttg cggcgcggat gcctggaact cgacgtagtc gacgtctttg
      781 gccagcccgc agcgctccag gagccccggg tagtagggat ggttgtagat gacggagatg
      841 gtggccagct cgccaaaccc ctccaccagg agtccctccg ggtccaggtc cgtgaagccg
      901 tggggcccgg tgatggtctg catgcccaac tccttgcccc acgcctcgac ggcggcgagc
      961 agggcccggg ctacctcgaa gtcttcgatg gtgtcgaacc agccgaagcg aaggttgcgg
     1021 gtgttgtact tgcggttggc cgcgtggctc aggatggccg ccacgcgccc caccacggct
     1081 ccgtctcgac gggccaggaa cagacgggct tccgcctcgt cgaacgccgg gttcttctct
     1141 cgggtgaagg tctccatctc gtcccggatg agcgggggca cccagtaggg gtttccggag
     1201 tacagccgca tggggaacgt tacgaaggct ttcagctccc gacgggagcc gaccgtcgag
     1261 acctcgacgc tcatgctgca acctctcttg tcctcaccgt aaaatgtatc gcaaacaccg
     1321 tcaaggacga gcctcacgct gcgcccggcg cagccgggcg cgggtatcca ttgccagcag
     1381 cgcgggcagg gtcaccagcg tgccggcggt ggtggccagg agcgtgaaca gcaccagcat
     1441 gccgaagtag accacgggac ggaacgaggc ggtggcaaaa accaggagcc ccacgatgat
     1501 ggaggcgctg gagagcagga tgggaagccc aatggcgccg agggtccgct ccagcgccag
     1561 gccgggatcc ccgggagagc gggccaggtc ccggcggaac tggatcacca ggtagatggc
     1621 gctgtccacg cccacgccga tggcgatgtt ggacaccatg atggtggtca tatccagggg
     1681 gatgcgcgtg agcgccatca tggagtagtt gaggagaagc ccggtgacca ggggcacgat
     1741 gggatacagc ccgtggcgca cggagcggaa gacccagatg gtgagcgcca ggatgcacac
     1801 gatcgagagc gccatggaca ggaggagcgt ccgacggagc gagtcggcga aggccaggtt
     1861 ccgaagcagg tcgccccaga ctaccggcgt ggcgccgatg gggttcgccg caagcacctg
     1921 gttcatcgac gcgagcaggg accggaaccg cgcctcgtcc atgtagtggg cggtcgtcga
     1981 gttgtagatg cggaagctga gagtgagccg gctgaagtcc ttgttcacga ggttggacag
     2041 caccgcggcc cccggcgacg agccgccgga tgccgtgatc acgcgggaga acaccgtgat
     2101 gatggcccgg ttggccggaa gctcgtccct tcccgtggcg gcccggttca gctcctggag
     2161 caggtccggc agcgacagcg cgtaggagat gtcgtggatc ccacgaagcg ccgtctccac
     2221 gccctgcacc ttcttcaaga tggccgggtc ctggaagtag ccggcagcgc gccccggcgc
     2281 gtcgaaggag accgagacct gctcgtagcc ccccgccgac cgatagattg cctgcatatc
     2341 ctggagcacg gggtcgctct gggggaagta gctggccggg tcggtgttga agaccaggag
     2401 gtggagcgtg gtggcaaaga ggatcacgac cgcgctgagg agacccagaa tgatggccgg
     2461 gtaccggttg gcgaaccgcg ccagggagcg catgagtcgg gtgaacgcgc cgtcgggctg
     2521 atgcgtgcgg accaccgggt ccttgaggag cgcgaacagc gccggcagga agaacagggc
     2581 caggaaggcg catgccaggg agccgaagct ggccgtcaga gcgaactggc gcgtctggcg
     2641 gatggaacca atgcacaagc tgaggaagcc aacgaccgtg gtcaggaacg ccatggcgat
     2701 gggctttgcc acggcgcggc tggcgcgcac gatccatccc ggcgcggtgc cctccacgcc
     2761 ggcaacgcgg aggtactcgt tcgtggtgaa gatgttgtac tcgttcccga agatcaggat
     2821 cagcggcggc gccacgatcg tgatcaggga gagcgggtag cccatcaccc ccatgaaccc
     2881 gaccgtccac accgtgccca ggatcacgga gaggagcggg agggcgatcg cccggaagga
     2941 ccggaacccc accacgtagc tcaggaggat gatgagcgcg gccagcgcca agaggcgcga
     3001 ggcgtcgctg gccagatgca tgccggtccg cacgctgagc gggatggtgc cggtcagaaa
     3061 cggcgtgagc ccccgcgctc gcatgccggc cgccacctgg tccacgcggt ccatgaacgg
     3121 cttgaaggag tcccgatgct cgcacgggaa gaatgcgatc agcatcgtcc cgtcggagga
     3181 gaccacgaga ttgcgggcgt agccggtgga caggaggcgc gcgcggaatg cgggtatatc
     3241 ctgcgggatc gaatccccgc cggccatggg ctgcaggagc agccgtccgt tgacccggtc
     3301 gaaggacacc aggttgaacg gggtcatggc ggattgaaca ccgggcatgg cggcgatgga
     3361 ccctaccgcc tggaagaatc ccgagagctg gtccggcgcg aagatgtctc cgccgggagc
     3421 ggaggctgtt gccgccagca ccaggaggtc cgggagcgcc tccgtcgtgc cggcatattc
     3481 cttgaggatc tggtttacct tgtcatcctg gggaagcagc gaggtgaagt ccggattgat
     3541 ctggacccgg accgcgaacc cggcgaggac cagggtggcg accgccgtca aagtcactac
     3601 catccgcgca tgcttcacgg aaaacgaaag gacgcgttcg acgggggtca tcgtcggcca
     3661 tgaaagccgg tttttgcggc gaaggtcaag gaacggcaag gttgcctcgg cttcctatgt
     3721 accggccgcg gtacccttcc gccaggcccg taggtatctt ctgccctgtg aagactcgca
     3781 ttcgacccgc tttggttctc ctgttgtcgg ctctgaccgt tcccctggcc gcgcagaaca
     3841 ccgggcagac ggtgagcgtc cgaagcggcg ggaaggacta cgtgtcatac cttgccgccg
     3901 gtcctggcgc cgagcagaag ccagggatcg tgctggtgca ctcgttcaac ggcctggagc
     3961 agggttacaa ggacatggtg gaccggttcg cctcggacgg gttcgtggtg ctggccgttg
     4021 gatggcagac cttcgagcag agtcccagtg acgccaccgt ggagcggctg gtgcgggatg
     4081 gggtagccat gctgaaggcg cgaagcgacg tggatccatc ccgcatcggc ctcaccggct
     4141 tctgcgccgg cggccgctac accatgctgc tcctgcccgt catcgacgat ttcgccgccg
     4201 gggttgcctg gtacgggttt ccctacagcg gcgggtccgc ggcgcagccc gtgaggccgg
     4261 tggacgagat cgaccggctg agccatcctc tgctcatgat ccacggtagc gcggaccagg
     4321 ccagccccat caccaacatc ttcaactacg ccacggcgct tcagaaggcg agcaagtact
     4381 tcgagctcaa ggtctacgaa gggcagccgc acggcttcat ggtgcagaac ggccagcttc
     4441 gcaaggacag catcgggctg gatgcctacc aggagatggt gaccttcttc cacaggaacc
     4501 tgggatagcg tgacggactg ggcggccgcg gtttccacgc ggctgggtcg gaccgacccg
     4561 gccagactgc agaacacgct catggcgatg agcgacaaga tctgcgcggt tgccctggcc
     4621 accctccccg aggagaggcg ggacgcggtc tatgcgctca tcgctcccac caaggcgcgc
     4681 cgcatccgtg aggagatccg actggagacc cggcgcagga ccgcgcgcgc cgtgcgggac
     4741 cgcatcatcc gggagttcct gaagagcttc gacgccgggc ccaagacgcg tcccacgatc
     4801 tacatccgac cgaagcggga ctcccggctg cggtgatccc ccgatcgatt tcagtgaaac
     4861 ccttcggcaa agacgcgaga gccggggaag cgctcatcga cgagcaaggt gacgtctcga
     4921 atcatcgcgg cgttgccgca tagatagaaa tcgtaaacac cggcaggtaa gcggtcccga
     4981 agccaggcca ccacgcgccc cgggtatgca ggatccgggt cttccgggcg atggggtccc
     5041 tccgtcagac atggcacgta tcgggcggcc gctttgctca gaagcacgcg gtagtgcagg
     5101 tcctccggcc tcttcactcc gtgaagcaga agggacggac acacgcccga cgccgccatg
     5161 gcaacgaagg gagccacacc ggttccggtt gccacgaaga ccgaggccac ggaagcgtcg
     5221 cgaaacacga agtagccatg gggtccgctc aggaccagtg aggcccccag gcgggtctcc
     5281 gcaaggagtc cggagacgac gccctgctcg accagccgca ccaggagccg gagggtcggg
     5341 gccgcagggc cgcaggccag cgtgtagtct ctctccacgg ggccacgatg gatgcgcacg
     5401 cgctggccgg ccgcgaacga gagaccaggc ggacgggcca gctcgagctg gaagacccga
     5461 ggggagagcc aggtccttcc caggagctcc gcggtgaggg acggtgcctc cgctgcgctc
     5521 aaccctgcca ggtcctctcg ggcgttccga agagctgatt gcagtaccgc gcggccacga
     5581 aaagataaaa ggacagccga ttcaggtacg ccaggatgtt ggccagctca ggatccggac
     5641 cggaagccgg cacggccaga cgcagcgcct gccgctccgc gcggcggcat acggcgcgcg
     5701 caacgtgcgc ccaggaggcc acgggatgcc cgccggccag gatgaagtgt tccagcgggg
     5761 ggagcgccgc ctggatccgt tcaacggcgg cctccagatg accggtaggc tcgggaccga
     5821 acggccgaag catgccacgg gacgcggacc cgggtgtggt ggccagccag gcgcccgccg
     5881 aaaggagctc accctggacc cgctggatct cggagccgag ccggctggct ccttccgtcg
     5941 tagccgcctc cgcgctcggg agcacggcag ccgacagcgc cgaggcgagc gcgcccagca
     6001 cgctccccaa ctcgtccagg gcgccgcagg cttcgatccg gtcgtggttc ttcgacaccc
     6061 gctcgccgct gaagaggctg gtcataccct tgtcccc
//
LOCUS       DASRZS010000089         7015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774 k141_46878,
            whole genome shotgun sequence.
ACCESSION   DASRZS010000089 DASRZS010000000
VERSION     DASRZS010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46878"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1080)
                     /locus_tag="VFH83_02180"
     CDS             complement(46..1080)
                     /locus_tag="VFH83_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485196.1"
                     /translation="MTSRERFDGFLSRTRIDRVPVQLQNLFLAAQAVRTPFSVVCRDA
                     ELVARGHLLEWEKYRQDGVIVDTGTHVAAESLGCAVTYEGDELPKVTGPAIETWDGWR
                     SLSIPDLLAAPSTVAVLDAVRILKREIGDHAVIIGTVDQGPFTLTTQIAGLEKTLLAL
                     ATQEAEAEIQAILELCAQFTLCFGMELAEAGADVIRMGDSIAGTDLISPTMYRAYALP
                     MQRWLAEEFRQVGIVFDFHICGNATPIVADMVSTGAAYIEIDEKTDLRVARAAVRDGC
                     GINGPISPRLLRFGTSGEVEASCRDVLTAWMPQGGLFFGPGCALVKDTPEENLRVLMD
                     SAERLGAYSS"
     gene            complement(1180..3150)
                     /locus_tag="VFH83_02185"
     CDS             complement(1180..3150)
                     /locus_tag="VFH83_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485197.1"
                     /translation="MRRPSVCVITLGASDLRTLLERSGLSVTVVDPGRRASKRRDLSR
                     FDALAILGGTTEQPLLLDPQLRSSVEAGIRAGARVFCEYCESIGGVYTAPPVPTRFER
                     IVVASEEPRIDGLRRGDLLEDQCNTRIAPYEFVTRYGPPLLQYTRALGHDRVESGVSA
                     TADAATVDAAAADAAGLPISERALWFHHPANLLICVFRLCNFVRARFSPTARWRSLVA
                     FVLEWLCGQPVPVDILEPRYHVLAFDPAVPLETQVSLSAQRAVRWFGKAGLLVDGGRG
                     GVMEGLGTEISADGHQQLRTIVRNDCTGETALAFFLHSMLTGRRESARIARRLIDWCF
                     DRMQVKDPGPLQGMMRWTPAAWQVCYADDNARVIIPQLLMNLYGGGRTHQEECVEALR
                     FLVRTTGTDGLRVMRTEGDLLDAQTVRDLSSGPGALPSAHYNAYYLGALLLAAKLTGI
                     EEFRRIGELGLGSIMAVYPNTRRELSETEELCRLILPLALLYWVTRKPEHRDWLYRVT
                     EDLQRFRHPSGAYLEWDTGHAAALSRNVGGEASLLMNNGDPVVDLLYSVNWLPMGFMQ
                     ACLVTGDERFRTLWQDAARFLVSCQIRSKDPLLDGAWTRAFDVELGEVFGSPGDVGWG
                     PWAIESGWTVAEIAAGLSAGLLEPRLRRFYRD"
     gene            3215..5191
                     /locus_tag="VFH83_02190"
     CDS             3215..5191
                     /locus_tag="VFH83_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HET6485198.1"
                     /translation="MKTPDLVQRQIHLDFHTSPLIPGVGQDFDARTFARTMQAAHVES
                     VTVFAKCHHGHLYYATRHPARHPTLPPRLDLLGGQVKALHALGIRAPIYISVLLDEFA
                     AAQHPEWVARRPDGTTVGGGPLQAAWRVMDMAGPYQEYLADQVREVLHRFQPVDGIFF
                     DICFDVESCTPAALSGMTREGLDPESPEDRKRYAASVTQAYLRRFSRMVVESSPRATV
                     YFNSRPLGGLTQDLRWMTHVEIEALPTGGWGYLYFPKNVRYARTFGRPTMGMTARFHK
                     SWADFGGLKPAAALRYEVSQMLAHGSVCSIGDQMHPRGVLDPAAYQLIGDAYAHAEAC
                     EPWTKGARPVAQIGVFMTGLGQARYHEEPGGSNDGAVRMLSQLKHQFDLIGADAPLER
                     YRLVILPDSIPVDEDLAGRLRAFLKGGGSLLLSGTSGLDAQGQPVLREAGVRANGMSP
                     FAVSYVRLGNAISHGAAATDHVMYEPSVRILPAAGASVLAKVVEPYFDRTWRHFSSHH
                     QTPPRPEASRWAAVVRKASVISIAYPIFKLYGTHANLAARSLVENCIRLLLPDRLVEI
                     DAPSAAEVTVTRQGSRRIVHLLYYPADRRTADLDIAEDTVPLRNVPLSLLSEKPPRRV
                     YCAPSGQPLIHDYGNGRVRVVVPVVEGHQMVVVE"
     gene            complement(5241..6239)
                     /locus_tag="VFH83_02195"
     CDS             complement(5241..6239)
                     /locus_tag="VFH83_02195"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HET6485199.1"
                     /translation="MSSFELLSPDAALGAAERAYAISLDGTVDAYPSYVNRVYGLRGD
                     DGARYVAKFYRPGRWTRDAILDEHRFLLDCTQAEIPVIAPLAGANGETLHAVSVADDA
                     GGDSPQTFLFTLFPRFGGRAFEPESDEDLERLGGILGRCHAVGSQREAVGRVVLGREL
                     TRSFVTELLDGALVHPECRDDFSQVCGETLEAIGPLFEGMKRIRIHGDCHRANILVRP
                     DTGLVLIDFDDMMSGPAVQDLWLILPGYASDSGREIELLLEGYERFAQFDRGTLALIE
                     PLRFMRMIYFLAWQARQRADYWFHQTYPDWGTEAFWIKETEDLRVQAEVIRAGLKD"
     gene            complement(6236..>7015)
                     /locus_tag="VFH83_02200"
     CDS             complement(6236..>7015)
                     /locus_tag="VFH83_02200"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET6485200.1"
                     /translation="GSMTEAEVNLRIAQAVVSQLQSAGVQVDLLPATVPPGYDADAFV
                     AIHADSGGRSLSGFKVSTPWRASRASKVLRDSVADTYSQVTGLPMDRYGISYNMRGYY
                     AFSYYRFLHAVAPSTPCMIIETGFLTSALDRQVIVDQPERAARGISEGIISYLGERAT
                     MRPSDLVPLVYAPLEVATDQAALRYYPRDGERVMGRLASGTLVRVFDERDGWAELMVF
                     GHFRLQGWMKLSDLRGVADFYGGTTKARALDPSAHGSPGSS"
BASE COUNT         1216 a         2449 c         2275 g         1075 t
ORIGIN      
        1 cgggattccg cggcggtggc gccccaacgg cggcgaacgt agcggtcagg aggaataggc
       61 tcctagccgc tccgcggagt ccatcaggac ccgcaggttc tcctccggcg tatccttcac
      121 cagggcgcag ccgggaccga agaacagccc tccctgcggc atccaggcgg tcagaacgtc
      181 acggcaggaa gcctcgacct cgcccgacgt gccaaaccgc aggagcctgg ggctgatggg
      241 tccgttgatg ccgcagccat cccggaccgc cgctcgggcc acccgcaggt ccgtcttttc
      301 atcgatctcg atgtaggcgg cccccgtgga gaccatatcc gcgacgatgg gcgtggcgtt
      361 tccgcagatg tggaagtcga acacgatgcc gacctgccgg aactcctccg ccagccaccg
      421 ctgcatgggc aacgcgtagg ctcgatacat cgttggcgaa atgaggtccg tccccgcgat
      481 cgagtctccc atgcggatca cgtccgcccc cgcctcggcg agctccatcc cgaagcacaa
      541 cgtgaactgg gcgcagagct ccaggatcgc ctggatctcc gcctccgcct cctgcgtggc
      601 aagggcgagg agtgtcttct ccaggccggc gatctgggtc gtgagcgtga atggcccctg
      661 gtccaccgtt ccgatgatca ctgcgtgatc gccgatctct cgcttgagga tccgcacggc
      721 gtccaggacg gcgacggtgg agggcgcagc cagcagatcc ggaatggaca ggctccgcca
      781 gccatcccag gtctcgatgg ccggtccggt aaccttgggc agctcatcgc cctcatacgt
      841 cacggcgcaa cccagtgact ccgcggccac atgggtcccc gtgtcgacga tcacgccatc
      901 ctgtcgatac ttctcccatt ccaggaggtg gccccgcgcc accagctcgg cgtcccggca
      961 aaccacggag aagggcgtcc gaaccgcctg agccgccagg aacaggttct ggagctgcac
     1021 ggggacgcgg tcgatcctcg ttcgggagag aaacccatcg aagcgttccc tgctggtcat
     1081 gcgagcccgg ccaagacacg gccctcctgt ggagcgcccg gtatagggtt ccgtgacagc
     1141 gctgtcaagc cgggaagggg agaggctgcg ccaggcagct cagtcccggt agaacctgcg
     1201 cagcctcggc tccagcaggc ccgccgagag gccggcggcg atctccgcga cggtccagcc
     1261 ggactcgatg gcccagggtc cccatcccac gtccccggga gaaccgaaca cttcgccaag
     1321 ctcgacgtcg aaggcgcggg tccacgcccc atccagaagt gggtccttgc ttcggatctg
     1381 gcaagatacg aggaaccgcg cagcatcctg ccagagggtc cgaaagcgct cgtcaccggt
     1441 gacgaggcag gcttgcatga atcccatggg gagccagttc acggagtaca gcagatccac
     1501 cacgggatcc ccgttgttca tgagaagaga cgcctctccc ccaacgttgc gactgagggc
     1561 cgccgcgtgc cctgtgtccc attccaggta agcgccgctc gggtggcgga accgctggag
     1621 gtcctcggtg acccggtaca accagtcgcg atgctccggc ttccgagtga cccaatacag
     1681 cagggccagg ggcagaatca gccggcagag ctcctcggtc tcgctcagct cccgtctggt
     1741 gttcgggtag acggccatga tgctcccgag acccagctcg ccgatccggc gaaactcctc
     1801 gatcccggtg agcttggctg ccaggagaag ggcgcccagg tagtaggcgt tgtagtgggc
     1861 gctgggaagc gcgccgggcc ccgatgagag gtctcggacg gtctgcgcat ccaggaggtc
     1921 cccttccgtc ctcatcacgc gaagcccatc cgtgccggtg gtccgcacca ggaaccgaag
     1981 agcctcaacg cactcctcct gatgcgtacg cccgccgcca tagaggttca tcaggagctg
     2041 cgggatgatg acgcgcgcgt tgtcatccgc gtaacagacc tgccaggcgg ccggagtcca
     2101 ccgcatcatg ccctgaaggg ggcccgggtc cttcacctgc atccggtcga agcaccagtc
     2161 gatgagcctc cgggcaatcc gggcactctc ccgccttccc gtcagcatgg aatgaagaaa
     2221 gaaggccagg gcggtctcgc cggtgcagtc gtttcggacg atggtcctga gctgttgatg
     2281 gccatccgcg gagatctccg tgcccaaccc ttccatcaca ccgcctcggc cgccgtctac
     2341 caggagcccg gctttgccga accaccgaac ggcccgttgg gcggacaggg acacctgggt
     2401 ctccagggga accgccggat cgaaggccag cacgtggtac cggggctcca ggatatccac
     2461 cggcaccggt tgtccgcaca gccactcgag gacaaaggcc accagggagc gccagcgcgc
     2521 cgtgggcgaa aaccgcgcgc ggacgaagtt gcacagcctg aatacgcaga tgaggagatt
     2581 ggccggatgg tggaaccaga gggcccgctc cgaaatgggc aggccggctg cgtccgcggc
     2641 ggctgcgtcc acggtggctg cgtcggcagt ggccgacacc cccgactcca cccggtcgtg
     2701 cccaagcgcc cgcgtatact ggagcagcgg cggcccatag cgggtcacga actcataggg
     2761 cgcgatccgc gtgttgcact gatcctccag aaggtcgccc ctgcgaagcc catcgatccg
     2821 aggctcctcg gaagccacga cgatgcgctc aaaacgcgtg ggtaccggag gcgccgtgta
     2881 aacgccaccg atgctctcac agtactcgca gaagacccgc gcgcccgccc ggatcccggc
     2941 ctctaccgaa gagcgaagct gaggatccag aagcagcggc tgctcggtcg tcccgcccag
     3001 gatggccagc gcatcgaacc tggacaggtc gcggcgcttc gaggctcgcc ggcccggatc
     3061 aacgacggtc accgagagcc cggatcgctc caggagcgtc ctcaggtcgc tcgcgccgag
     3121 ggtaatgacg cagacggatg gccttctcat tcgcgcaaca gtagctctca cgccttgcgg
     3181 cggcaatccc gggccggtag aatccccatc acccatgaag acacccgacc tcgtccagcg
     3241 ccagatccac ctggactttc ataccagccc gctcatcccg ggggtgggcc aggacttcga
     3301 cgcgcgcacc ttcgctcgca ccatgcaggc cgcccacgtg gagtcggtca cggtgttcgc
     3361 caagtgccac cacgggcacc tctactacgc cacccggcac ccggcgcgcc accccacgct
     3421 ccccccgcgc ctggacctcc tgggcggcca ggtgaaagcg ctgcatgcgc tgggcatccg
     3481 cgcccccatc tacatctccg tgctgctgga cgagttcgcc gcggcgcagc acccggagtg
     3541 ggtggcgcgc cggccggacg ggaccaccgt gggcggcggg ccgctgcaag ccgcgtggcg
     3601 cgtcatggac atggcaggac cctaccagga gtacctggcc gaccaggttc gggaagtcct
     3661 tcaccggttc cagcccgtgg acgggatctt cttcgatatc tgtttcgacg tagagagctg
     3721 cacgccggcc gctctctccg gcatgacccg ggagggactg gatccggaga gccccgagga
     3781 tcgaaagcgc tacgccgcct ccgtcaccca ggcatatctg cgccggttct cacggatggt
     3841 cgtggagagc tcccctcggg caaccgtgta cttcaactcg cggcctctcg gcggtctgac
     3901 ccaggacctg cgctggatga cgcacgtgga gatcgaggcc cttcccaccg gcggctgggg
     3961 ctacctctac ttccccaaga acgtccgcta cgcgcggacc ttcgggaggc ccaccatggg
     4021 catgaccgcg cggttccaca agagctgggc ggatttcggc ggcctgaagc cggccgccgc
     4081 gctgcgctac gaggtgagcc agatgctggc ccacggatcc gtctgctcca tcggggacca
     4141 gatgcacccg cgcggcgttc tggatcccgc cgcgtatcag ctcatcgggg acgcctacgc
     4201 tcacgcggag gcatgcgagc cctggaccaa aggggcccgt ccggtagcgc agatcggggt
     4261 tttcatgacg gggctgggcc aggcgcgcta tcacgaggag ccgggcggaa gcaacgacgg
     4321 cgccgtgcgg atgctcagtc agctcaagca ccaattcgac ctgatcggcg cggacgctcc
     4381 tctcgaacgg taccggctcg tgatcctccc ggacagcatc cccgtggacg aggatctggc
     4441 cggccgactg cgggccttcc tgaagggcgg cggctccctc ctcctcagcg gcaccagtgg
     4501 gctggatgcg caaggccagc ccgtgctgag ggaggccggc gtgcgggcca acgggatgag
     4561 cccgttcgcg gtcagctacg tccggcttgg taacgccatc tcgcatgggg ccgcggcgac
     4621 ggaccacgtg atgtacgagc catccgtgcg catcctcccg gccgccggcg cctccgtgct
     4681 ggccaaggtc gtggaaccgt acttcgaccg cacctggagg catttcagct cgcatcacca
     4741 gaccccgcct cgacccgaag ccagccgttg ggccgcggtc gttcgcaagg cctccgttat
     4801 cagcatcgcc tatccgatct tcaagctcta cgggacgcac gccaacctgg cggcccgaag
     4861 tctcgtggag aactgcatcc gcctgctcct ccccgaccga ctggtggaga tcgatgctcc
     4921 cagcgcggcc gaggtcacgg tgactcgcca gggctcccga aggatcgtgc acctgctgta
     4981 ctacccggcg gatcgccgca ccgccgatct ggacattgcc gaggacacgg tgccgcttcg
     5041 caacgtgccg ctgtctctgc tgagcgaaaa gccgccgcgg cgcgtgtact gcgccccgtc
     5101 gggacagccg ctcatccatg attacgggaa cgggcgggtc cgggtggtgg tgcccgtcgt
     5161 ggagggccac cagatggtgg tggtggagta gcaagcagag gacgcggcgg acgggggccg
     5221 ccgacctcct cggcaggccc tcagtctttc agcccggccc ggatcacctc ggcctgcacg
     5281 cggaggtcct ccgtctcctt gatccagaac gcctcggtcc cccagtccgg ataggtctgg
     5341 tggaaccagt agtcggcgcg ctgccgtgcc tgccaggcaa ggaagtagat catccgcatg
     5401 aagcgaagcg gctcgatgag cgccagggtt ccgcggtcga actgcgcgaa ccgctcgtag
     5461 ccttccagga ggagctcgat ctctcgaccg ctgtctgacg cgtagccggg aaggatgagc
     5521 cacaggtcct gcaccgccgg accgctcatc atgtcgtcga agtcgatgag gaccagaccg
     5581 gtgtccggcc gcaccaggat gttggctcga tgacagtccc catggatgcg gatccgcttc
     5641 atcccctcga acagagggcc aatggcttcc agggtctcac cgcagacctg ggagaagtca
     5701 tcccgacact ccgggtggac cagcgctcca tccagaagct ccgtgacaaa ggaccgggtc
     5761 agctcccgcc ccagcaccac ccggccgacc gcttccctct gggaccccac cgcgtggcag
     5821 cgtcccagga tgcctcccag ccgctccaga tcctcgtcgg attccggctc gaacgcccgg
     5881 ccgccaaagc gcggaaacag ggtgaagagg aaggtctgcg gcgagtcgcc gcccgcgtcg
     5941 tccgccacgc tcaccgcgtg cagagtctct ccgttcgcgc cggccagagg cgcgatcaca
     6001 gggatctccg cctgcgtgca atcgaggagg aaccgatgct cgtccaggat cgcatcccgc
     6061 gtccagcggc cagggcggta gaacttcgcc acgtatcggg ccccgtcatc gccccgaagc
     6121 ccgtacacac gattgacgta actgggatag gcgtccaccg tcccgtccag cgagatggcg
     6181 taggcccgct cggcggcgcc cagcgccgcg tccggcgaga ggagctcgaa ggagctcacg
     6241 atgaccccgg tgacccgtgg gctgatgggt ccagcgcccg cgccttcgtg gttccgccgt
     6301 agaagtccgc gactccgcga agatcggaga gcttcatcca gccctggagg cggaagtgcc
     6361 cgaagaccat gagctccgcc cagccgtccc gctcgtcgaa aacacggacc agggtgccgg
     6421 acgccagcct gcccatgacg cgctccccgt cccgggggta gtaccgcagg gcggcctggt
     6481 ccgtggctac ttccagcggc gcgtagacca gggggaccag gtcggagggc ctcatcgttg
     6541 cgcgctcacc caggtaggag atgatccctt cgctgattcc gcgcgccgcg cgctccggct
     6601 ggtccacgat gacctgacga tccaaggccg aggtcaggaa gccggtctcg atgatcatac
     6661 acggcgtgct cggcgccacg gcgtgaagga accggtagta gctgaaggcg tagtagccgc
     6721 gcatgttgta gctgatcccg taccggtcca tcggcaggcc cgtgacctgg ctgtaggtat
     6781 ccgccacgct gtccctgagc accttggacg cacgggaggc gcgccagggt gtggagacct
     6841 tgaacccgct gagactccga cctccggaat ccgcgtggat ggcgacgaag gcatcggcat
     6901 cgtagcctgg cggcaccgtt gccggcagga ggtccacctg gacgccggcg gactggagtt
     6961 gggaaacgac cgcctgggcg attcggaggt tcacctccgc ctcggtcatg gaacc
//
LOCUS       DASRZS010000090         4008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5437112, whole genome shotgun sequence.
ACCESSION   DASRZS010000090 DASRZS010000000
VERSION     DASRZS010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5437112"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VFH83_02205"
     CDS             <1..665
                     /locus_tag="VFH83_02205"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HET6485201.1"
                     /translation="GSVNAMHDSLSPLGGMVPMVNMMLGEIIYGGVGAGMYGILVFLF
                     IAIFIAGLMVGRTPEYLGKKIETREVQLSVLAVIGQTVVILFLAAAAAVSKVGLSSLT
                     NAGPHGLSEILYAYASGVGNNGSAFAGLNANTPFWDATMAFAMLVGRFIVIVPVMAIA
                     GSLGAKTPIPPSVGTFATNNTTFVVLLVLTVLILAGLNFFPALTLGPIVEHFLALKGV
                     VF"
     gene            676..2751
                     /gene="kdpB"
                     /locus_tag="VFH83_02210"
     CDS             676..2751
                     /gene="kdpB"
                     /locus_tag="VFH83_02210"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HET6485202.1"
                     /translation="MRQEIRLMSFPVLRRAVAMAFQKLNPRVEIRNPVMFMVWIGSVF
                     ISVVFIVNAIQGRFDGFNLQIAIWLWFTVLFANFSESIAEIQGKARADALRAMRTKTV
                     ARRIAANNGHEERVASIELRVGDRVICETGDVIPGDGEVVEGIASVDESAITGESAPV
                     IRESGGDRIAVTGGTKVISDRIVVRITAEQGKTFVDRMISLVENATRQKTPGEIALFI
                     LLSAFSVIFLAAVGTLWFFSDYSVKQSGSVVFITTAVLFSLLVCLIPTTIGGLLSAIG
                     ISSVSRLIKKNVLALSGKAVDAAGDIDVMLLDKTGTVTMGNRMAAAFLPAKGVPESEL
                     AETALLASLADETPEGRSIVNLARKNYPIKAASMQEMGGRFVPFSAKTRMSGVDFSDQ
                     RRQIRKGAAEAVRVWVSTAGGSYPPEVQAEVSRISAEGGTPLVVARGSRVLGAVYLKD
                     VVKGGIRDRLLHLRQMGIRSIMITGDNPLTAATIAAEAGVDDYIAEATPQAKLERIRA
                     EQRSGHLVAMIGDGTNDAPALAQADVGVAMSSGTQVAREAGNMVDLDSNPTKLIDIVE
                     VGKEQLMTRGALTTFSIANDVAKFFAILPAMFVTLYATAGSPGPLSALNVMHLASPQS
                     AVLSAVIFNALIIVVLIPLALRGVRYRAIGAIRILRRNLLIYGVGGIIAPFVGIKLID
                     LLVHVLGLA"
     gene            2766..3335
                     /gene="kdpC"
                     /locus_tag="VFH83_02215"
     CDS             2766..3335
                     /gene="kdpC"
                     /locus_tag="VFH83_02215"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014761733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HET6485203.1"
                     /translation="MKPIRTAIIVTIFWTIAVGGIYPLFMTGIGTVFFPHQVGGGLVD
                     RGGKVVGSELIGQAFESDRYFHPRPSTINYDASASGASNEGYTSADLKKNYDQRKADW
                     QKRFGTGEPPMDMLFASGSGLDPDISPQAAEGQVNAVAAARHLSADQASQLLALVEGQ
                     VKAPQLGFLGEPRVNVLELNLALDKTFPG"
     gene            3343..3999
                     /locus_tag="VFH83_02220"
     CDS             3343..3999
                     /locus_tag="VFH83_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET6485204.1"
                     /translation="MRSPAWFALPLVLWLVGCGVNGQGSFYDLAATGSAQQVLAAIQS
                     GADAREQDPLGVTPLMTAAASNPDPYVLQVLMAAGAGVNQRDTMGATPLMYAVKANRN
                     PAIASALLAAGARVEDRDFLDQTPLMLAARWNASPDVLRTLIEAGARIDDRDLFGVTA
                     LMLAAEHTGNPEVVQALLDAGADATPRSWGGKNAYELAGDNPALQDTSALRALASAAR
                     "
BASE COUNT          683 a         1292 c         1345 g          688 t
ORIGIN      
        1 acggctccgt gaacgccatg cacgacagcc tgtccccgct gggcggcatg gtccccatgg
       61 tgaacatgat gctcggggag atcatctacg gtggggtcgg cgcggggatg tacgggatcc
      121 tggtgtttct gttcatcgcg atcttcatcg ccgggctgat ggtgggccga acgccagaat
      181 acctgggtaa aaagatcgag acccgagagg tccagctctc ggtcctggcc gtgatcggtc
      241 agacggtcgt gatcctgttc cttgccgcgg ccgccgcggt gtccaaggtc gggctgtcct
      301 cgctgaccaa cgctgggcct cacgggctct cagagatcct ctatgcctac gcttcgggtg
      361 tgggcaacaa cgggagcgcc ttcgcgggct tgaacgccaa caccccgttc tgggatgcca
      421 ccatggcctt cgccatgctg gtgggtaggt tcatcgtgat cgtgccggtc atggcaatcg
      481 cgggcagcct gggagcaaag acgcccatcc cgccgagcgt aggcacgttc gccaccaata
      541 acaccacctt cgtcgtcctc ctggtcctga cggtcctgat cctggcgggg ctgaacttct
      601 tcccagccct gaccctgggg ccgatcgtgg agcacttcct ggccctcaag ggcgtcgtgt
      661 tctaggagcc gcgtcatgag acaagagata cgtctgatga gctttcccgt cctgagacgg
      721 gccgtcgcga tggctttcca gaagctgaat ccccgcgtgg agatccgaaa cccggtgatg
      781 ttcatggtct ggatcggatc cgtgttcatc tcggtggtgt tcatcgtgaa tgccatccag
      841 ggacggttcg acggcttcaa cctgcagatc gccatctggc tctggttcac ggtcctgttc
      901 gccaacttct cggagtccat tgccgagatc cagggcaaag cccgcgccga cgcgctgcgc
      961 gccatgcgga ccaagacggt ggcccgacgg attgcggcca acaacgggca tgaggagcgc
     1021 gtcgcctcca tcgagctacg ggtgggcgac cgggtgatct gcgagaccgg ggacgtgatc
     1081 cccggggacg gcgaggtggt cgaagggatc gccagcgtgg acgagtccgc catcacggga
     1141 gagtcggcgc cggtcatccg ggagtccggc ggggaccgaa tcgccgtcac cggcggcacc
     1201 aaggtcatca gtgaccggat cgtggtaagg atcaccgcgg agcagggcaa gaccttcgtg
     1261 gaccggatga tctccctggt ggagaacgcc acccgccaga agacgccggg agagatcgcg
     1321 ctcttcatcc tgctctccgc gttctccgtg atcttcctgg cggccgtggg cacgctgtgg
     1381 ttcttctcgg attacagcgt gaagcagtcc ggcagcgtgg tgttcatcac caccgcggtc
     1441 ctgttctctc tgctggtctg cctgatccct accaccatcg gaggcctgct gtccgccatc
     1501 gggatcagca gcgtgagccg gctcatcaag aagaacgtgc tcgctctgag cggcaaagcg
     1561 gtcgacgccg cgggagacat cgatgtcatg ctcctggaca agaccggcac cgtcaccatg
     1621 ggcaaccgga tggcggcggc gttcctgccc gccaaaggcg taccggagag cgagctggcg
     1681 gagacggcgc tcctggcctc cctggccgat gagacacccg aggggaggtc cattgtaaac
     1741 ctggctcgca agaactaccc gatcaaggcg gcgagcatgc aggagatggg cggccgattc
     1801 gttccgttct ccgcgaagac gcgcatgagc ggagtggact tctccgacca gcgtcggcag
     1861 atccgcaagg gggccgcaga ggcggtccgg gtatgggtct ccacggcggg aggctcgtac
     1921 cctccggagg tccaggcaga ggtcagccgc atctcggcgg agggcggaac ccccctggtc
     1981 gtagcccggg gaagccgcgt gctgggtgcg gtgtacctca aggacgtcgt gaaaggcgga
     2041 atccgggatc ggctcctgca cctgcggcag atgggaatcc gttccatcat gatcaccggc
     2101 gacaacccgc tgaccgcggc aaccatcgcg gccgaggccg gcgtagacga ctacatcgcc
     2161 gaagccaccc cgcaggcgaa gctggagcgc atccgggcgg aacagcgatc gggacacctg
     2221 gtggccatga tcggggatgg gaccaacgac gcccccgcgc tggcgcaggc ggacgtcggc
     2281 gtggcgatga gctcgggcac gcaggtggct cgggaagccg gcaacatggt ggacctggac
     2341 tccaacccga ccaagctcat cgacatcgtg gaggtgggca aggagcagct catgacgcgc
     2401 ggagcgctca ccaccttcag catcgccaac gatgtggcca agttcttcgc catcctgccg
     2461 gccatgttcg tcacgctgta cgcgaccgcg ggcagcccgg ggcccctctc ggccctgaac
     2521 gtgatgcacc tggcgtcgcc ccagagcgcg gtgctctccg cggtcatctt caacgcgctc
     2581 atcatcgtcg tcctcatccc gttggcgctt cggggcgtgc ggtatcgggc catcggcgcc
     2641 atccgtatcc tgcgccgaaa cctgttgatc tacggcgtgg gtgggatcat cgcgcctttc
     2701 gtcggcatca agctgatcga cctgctggtc cacgtgctgg gcctggcata agggggaacg
     2761 gaaccatgaa accgattcga acggctatca tcgtgacgat cttctggacc atcgcggtgg
     2821 gaggcatcta tcctctgttc atgacgggga tcggcaccgt cttcttcccc caccaggtgg
     2881 ggggcggcct ggtcgaccgg ggcgggaagg tcgtgggatc cgagctgatc ggccaggcgt
     2941 tcgagtccga ccggtacttc cacccgcgcc cctcgacgat caactacgac gcctccgcct
     3001 ctggcgccag caacgaggga tacaccagcg ccgatctgaa gaaaaactac gatcagcgca
     3061 aggccgactg gcagaagagg ttcggcacgg gagagccgcc catggacatg ctgttcgcct
     3121 ccggcagcgg cctggacccg gacatcagcc cccaggcggc ggagggccag gtgaatgcgg
     3181 tggccgccgc acggcacctc tccgcggatc aagcctccca gctccttgcc ctggtcgagg
     3241 gacaggtgaa ggctcctcag ctcggattcc tgggtgagcc gcgggttaac gtcctcgagc
     3301 tgaatctcgc cctggacaag acgttccccg ggtaggaccg ccatgaggag ccctgcctgg
     3361 tttgcgttgc ctctcgtgct ctggctcgtg gggtgcggcg taaacggcca gggctccttc
     3421 tatgatcttg cggccacggg ctcggcgcag caggtcctgg ctgccatcca gagcggcgcc
     3481 gacgcgagag aacaggaccc cttgggggtg actccgttga tgacggccgc ggcatccaat
     3541 ccggatccct atgtgctgca ggtcctgatg gctgccgggg cgggggtcaa ccagcgggac
     3601 accatgggtg ccacgccgct catgtacgcg gtcaaggcga accggaatcc cgcgatagcg
     3661 agtgctctgc tggccgccgg cgcccgggtg gaggatcgcg acttcctgga ccagacgccg
     3721 ctcatgctgg cggcgcgctg gaacgcgagc cccgacgtct tgcgcacgct tattgaggcg
     3781 ggcgcccgga tcgacgaccg ggatctcttc ggcgtcacgg cgctcatgct tgccgccgag
     3841 cacaccggca atcccgaagt ggtccaggct ctcctggacg ccggcgcgga tgcgacgccg
     3901 cgaagctggg gcggaaaaaa cgcgtacgag ctggccgggg acaatcccgc gctgcaagac
     3961 acgtcggccc tgcgggccct tgccagcgcc gcccgttagg catcggcg
//
LOCUS       DASRZS010000091         4483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1587801, whole genome shotgun sequence.
ACCESSION   DASRZS010000091 DASRZS010000000
VERSION     DASRZS010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587801"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="VFH83_02225"
     CDS             complement(<1..1018)
                     /locus_tag="VFH83_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET6485205.1"
                     /translation="MNAPAPAELPDSRYRKIFEYSAISLWEEDITRLRAWLAGLRRQP
                     GFRLRDHLARRPDLLQEAVKLIDVTDVNAASLRLFEAENKSQLLGPLSVVLDAVSRRA
                     LAETIVAIDEGRSDVEAESSALTLKGNRLEILVRTRIPPAGADYDRMLVTIVDITERS
                     RAEAELAWERSLFDALMGNLPDLVYFKDPSGRFLRISASTARALGLGSPSEGIGKTDW
                     DFFTEEYAIRAHEEEQRVMRTGIPLHDVEDEQSYTDRPNTWALTSIMPLMGPQGAVAG
                     TFGISHDITRRKELELSSRRFAALVEFSDDAIVGMDLDRRITVWNRGAERVYGYTAAE
                     MIGTR"
     gene            1150..1611
                     /locus_tag="VFH83_02230"
     CDS             1150..1611
                     /locus_tag="VFH83_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6485206.1"
                     /translation="MKPTETAVQQTVDVLLDALPAVWDRIRSRFRAAATGTFGITLEQ
                     FHVLRHIRKGYQSVGELAEKRQISRSAISQAVEVLVEKGLVTRQQQSADRRCFRLGLT
                     PHAAAVLEANSHEIRGWMGQTLAGSGVDELKRIQSVLATLKKTFAPEESRA"
     gene            1625..3367
                     /locus_tag="VFH83_02235"
     CDS             1625..3367
                     /locus_tag="VFH83_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485207.1"
                     /translation="MTGPQTILSYVRPYRRWALFSLVMLLAMVVLDLAVPRLVQQIID
                     QGIRRKDLAVVLRTSALMILIAVLDTVATILNSNTSVRVGESVARDLREAVFVKIQRF
                     SYGNLDRFSTGKLMVRLTSDASAVQRLTQITLRIGTRGPLTIIGSILLMFLDSPVLAA
                     SLIPILVVAAAAIVFFSIRMEPQFRAVQQRLDRLNTVLQENIAGARLVKAFVRADHEA
                     ERFGGANEELMGSTVKVMQFMSSMNPALTLLVNLALVLVVWLGGLSTIRGTLSVGQIV
                     AFVNYLLASLNPLIMMTNLSNVWANGLASAKRINEVLDAVPEVPEAPDAVHLDGQAKG
                     SVSFRQVRFHYNGNADLSVLEDICLHAEPGQTVALLGATGSGKSSLVNLIPRFYDASC
                     GSVSVDGIDVRKIQEDSLLEHVAVVPQETILFSGTVRDNIRYGRPEATHGEVEAAAQA
                     AQAHEFILRLPEGYDTHIEERGGNLSGGQKQRIAIARALVTRPSILILDDSTSAVDVE
                     TEGKIHGALAEHLRGCTVFLVAQRISTVLNADTILVIDRGRVVAQGRHAELLQTSPIY
                     REIYDSQLGGGIHA"
     gene            3360..>4483
                     /locus_tag="VFH83_02240"
     CDS             3360..>4483
                     /locus_tag="VFH83_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485208.1"
                     /translation="MRESLPPTPDVRSSMQRRGGRPAKFEKAQNPRRALTRLVLYLKP
                     YPASLGLVTGCVFLYTLLGLVGPYLLGQAIDTFIGGKNVAGLLRTVVLMVIVYGAGNA
                     LQVAANWVMARVAQRALKVMRGDLFAHLQTLSMHFFDTHPAGGLMSRLTNDIDAINQA
                     VSQNVTSLVASALSMVGILIAMFVLNHWLALASLLVVPIMIWFTRFVSRYTRKGFRDL
                     QKSLGELNSVTEEAISGQKVIKAFRRNESVLEAFRAQNEAVFRAGVFANSYALLLMPL
                     TAVLGTFFVIVLAGVGGWLALQGLLSVGMIATFITYGQNFTSPLRQIANLYNSIQAAL
                     AGAERVFEIIDTPSEMTDPPDAPALSSLRGDVSLRGVQFS"
BASE COUNT          751 a         1519 c         1427 g          786 t
ORIGIN      
        1 tccgggtccc gatcatctca gcggcggtgt acccatatac ccgctcggcg ccccggttcc
       61 acacggtgat gcgccgatcg agatccatgc ccacgatcgc gtcatcggag aactccacga
      121 gagccgcgaa ccgtcgggag ctcaactcca gctccttgcg gcgcgtgatg tcgtgcgaga
      181 ttccgaacgt ccccgcgacc gcgccctggg ggcccatcag gggcatgatc gaggtgagcg
      241 cccaggtgtt cggccggtcc gtatagctct gctcgtcctc cacgtcgtgc agggggatcc
      301 cggtccgcat cacccgctgc tcctcctcgt gcgctctgat ggcatactcc tcggtgaaga
      361 agtcccagtc cgtcttcccg atgccctccg agggcgaacc cagccccaac gcccgcgccg
      421 tcgaggcact gatgcgcagg aagcgaccgg aggggtcctt gaaatacacg aggtcaggga
      481 ggttccccat cagggcgtcg aacaggctgc gttcccaggc cagttcggcc tcggcgcggc
      541 ttcgctccgt gatgtcgacg atggtgacca gcatgcggtc atagtcggct cctgccggag
      601 ggatgcgcgt gcggaccagg atctcgagcc ggttgccctt gagcgtcaac gcggaggact
      661 cggcctccac gtcgcttcgg ccctcgtcga tggcaacgat cgtctccgcg agcgcgcgcc
      721 gggataccgc gtccaggacg acggaaagcg ggccgagaag ctgcgatttg ttctcggcct
      781 cgaagaggcg gaggctcgcg gcgttcacgt cggtgacgtc gatcagcttc accgcctcct
      841 ggagaaggtc ggggcgccgc gccagatggt cccgcaggcg gaagcccggc tgacggcgca
      901 ggccggccag ccaggctcgt agacgcgtta tgtcctcctc ccacagcgag atggcggagt
      961 actcgaagat cttgcggtac ctcgaatccg gcagttccgc gggcgctgga gcattcatga
     1021 ggcgctcttc tcccgatctc aggataccct gaaacgcggg cgtcggcacc cacttgcgcg
     1081 aaccccgttc cggaggatat attgtttagt gtcttaacag ataagaccct aaacagaaag
     1141 aggaccgcaa tgaaaccgac cgagaccgcc gtgcagcaga ccgtggacgt cctcctggac
     1201 gccctccccg ccgtctggga ccgcatccgc tcgcggttcc gcgcggcggc cactggaacg
     1261 ttcggcatca cgctggagca gttccacgtg ctccgtcata tccgcaaggg ctaccagtcc
     1321 gtgggggagc tggcggagaa gaggcagatc agccgctcgg ccatcagcca ggcggttgag
     1381 gtgctcgtcg agaaaggcct ggtcacccgc cagcagcaga gcgcggatcg gcgctgcttc
     1441 cggttggggc tgacgccgca tgccgcggcg gtgctggagg cgaactccca cgagatccgc
     1501 ggctggatgg ggcagacgtt ggcgggcagc ggcgtggacg agctgaagcg gatccagagc
     1561 gtcctcgcga cgctcaagaa gacctttgcg cctgaggagt cccgagcatg aacggttgcg
     1621 agccatgacc ggcccccaga ccatcctgtc ctacgttcgg ccctatcgcc gctgggcgct
     1681 cttctctctg gtgatgctcc tggccatggt ggtgttggac cttgccgttc cgcgcctcgt
     1741 ccagcagatc atcgaccagg ggatccgtcg caaggacctg gccgtggtgc ttcggacctc
     1801 ggcgctcatg atcctgatcg ccgtcctgga caccgtggcg accatcctga acagcaacac
     1861 ctccgtgcgg gtcggagaga gcgtggcgcg ggacctccga gaggcggtct ttgtgaagat
     1921 ccagcgcttc tcctacggga atctcgaccg gttctcgacg ggcaagctga tggtgcggct
     1981 gaccagcgac gcgagcgcgg tccagcgcct gacgcagatc accctgcgca tcggcacccg
     2041 cggcccgctc acgatcatcg gaagcatcct actcatgttc ctcgacagcc ccgtactggc
     2101 cgcgagcctg atccccatcc tcgtcgtcgc ggccgcggcc atcgtcttct tcagcatccg
     2161 catggagccc cagttccgcg ccgtccagca gaggctggac cggctgaaca cggtcctcca
     2221 ggagaacatc gccggtgcgc ggctggtgaa ggccttcgtc cgggcggatc acgaggcgga
     2281 gcgcttcggg ggcgccaacg aggagctcat ggggagcacg gtgaaggtca tgcagttcat
     2341 gtcctccatg aaccccgccc tcacgctgct cgtaaacctg gccctggtgc tggtggtatg
     2401 gctcggcggg ctctcgacca tccggggcac cctctccgtg ggtcagatcg tggccttcgt
     2461 gaactatctg ctcgcctccc tgaacccgtt gatcatgatg accaacctct ccaacgtctg
     2521 ggccaacggg ctggcttcgg ccaagcggat caacgaggtc ctggacgcgg tccccgaagt
     2581 cccggaagct ccggacgcgg tgcacctgga cggccaggcc aagggcagcg tgagcttccg
     2641 gcaggttcga ttccactaca acggcaacgc cgatctgtcc gtgttggagg acatctgcct
     2701 tcatgcggag cccggtcaga ccgttgccct cctgggcgcg acgggctcgg gcaagtcgag
     2761 cctggtcaac ctcatccccc gcttttatga cgcgtcgtgc ggctcggtat cggtggacgg
     2821 gatagacgtt cggaagatcc aggaagattc ccttctggag cacgtcgccg tggttccgca
     2881 ggagaccatc ctcttctctg gcacggtgcg ggacaacatc cgctacggtc ggccggaggc
     2941 gacccacggg gaggtggagg cagccgccca ggcggcgcag gcccacgagt tcatcctgcg
     3001 tcttcccgag ggctacgata cccacatcga ggagcgcggc gggaacctgt ccggcggtca
     3061 gaagcagcgc atcgccatcg cccgagcgct cgtcacccga ccgtccatcc tcatcctgga
     3121 cgacagcacc agcgccgtgg acgtggagac cgagggcaag atccacggcg cgctggccga
     3181 gcacctgaga ggctgcaccg tgttcctggt ggctcaacgg atcagcacgg tgttgaacgc
     3241 cgacaccatc ctggtgatcg accggggccg cgtggtagcg cagggaagac acgcggagct
     3301 gcttcagacg agccccatct atcgggagat ctatgactcc cagcttggag gcggcatcca
     3361 tgcgtgagtc cctgcccccc accccggacg tccgctccag catgcagcgg cgaggcggaa
     3421 gaccggcgaa gttcgagaag gcgcagaacc cgaggcgggc cctgacgcgg ctcgtgctgt
     3481 atctgaagcc ctacccggca tcgctcggcc tggttaccgg ctgcgtcttc ctctataccc
     3541 tgctcggcct ggtcgggccc tacctcctgg gccaggccat cgataccttc atcgggggca
     3601 agaacgtggc cgggctcctg cgtaccgtcg tcctgatggt catcgtctac ggggccggca
     3661 acgcgctgca ggtcgctgcc aactgggtca tggcccgcgt ggcccagcgc gcgctcaagg
     3721 tgatgcgggg ggaccttttc gcccacctcc agaccctgtc gatgcacttc ttcgacacgc
     3781 acccggcggg cgggctcatg agccggctca cgaacgacat cgacgcgatc aaccaggcgg
     3841 tctctcagaa cgtgacctcc ctggtggcca gcgcgctctc catggtgggg atcctcatcg
     3901 ccatgttcgt cctcaaccac tggctcgcgc tcgccagcct cctggtcgtg ccgatcatga
     3961 tctggttcac ccggttcgtg tcccggtaca cccgcaaggg attccgcgac ctccagaaga
     4021 gcctgggaga gctcaacagc gtcacggaag aagcgatcag cggtcagaag gtgatcaagg
     4081 cgttccgacg caacgagtcg gtccttgagg ccttccgggc tcaaaacgag gcggtcttcc
     4141 gcgcgggtgt ctttgccaac tcctacgcgc tcctgctcat gccgctgacc gcggtccttg
     4201 gcaccttctt cgtcatcgtc cttgccggtg tgggcggctg gcttgccctg caggggctcc
     4261 tcagcgtcgg gatgatcgcg acgttcatca cgtacggaca gaacttcacc tcaccccttc
     4321 gacagatcgc caacctctac aactccatcc aggccgcgct ggccggagcc gagcgtgtct
     4381 tcgagatcat cgacacccct tcagagatga cggacccgcc ggatgcccct gccctttcga
     4441 gcctccgcgg ggacgtgagc ctgaggggag tccaattctc cta
//
LOCUS       DASRZS010000092         6164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774 k141_49677,
            whole genome shotgun sequence.
ACCESSION   DASRZS010000092 DASRZS010000000
VERSION     DASRZS010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49677"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VFH83_02245"
     CDS             <1..605
                     /locus_tag="VFH83_02245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HET6485209.1"
                     /translation="RFRSEPRLTLICGDFLDTWENAVRDHGRPSKILGNLPYSSASQM
                     VAQILESGIQPQRLLVTVQRELGDRMASPPSTKSYSAFSVLCQACCEVIVRGELNPGS
                     FYPPPNVVSAIVELRPRQDAPSGPALGLLTRITRALFASRRKTLRNNIGRAGLDTGRL
                     LEALDVLGIDGGARAEELSPGDYVRLAERLAGTPTGSAGL"
     gene            complement(583..1461)
                     /gene="dapA"
                     /locus_tag="VFH83_02250"
     CDS             complement(583..1461)
                     /gene="dapA"
                     /locus_tag="VFH83_02250"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET6485210.1"
                     /translation="MKLEGVYTALVTPMASDGSLDEKALRRLVDFQIEGGVQGLVPVG
                     TTGESPTLDGDECKRVIQIVVEQSRGRVPVIAGAGSNATAEAIEYTRDAKEVGAVATL
                     QVAPYYNKPTSDGFLRHFRAIADAVDLPLVVYNIAGRTGKNIDNPTMLELARHRNIVA
                     VKEASGDIPQVMELILRKPGAFSVLSGDDNLVLPIMALGGTGVISVASNLVPDRMARF
                     VNAMLKEDWAAARSMHYELLPLFKALFVETNPIPIKAAMAMKGLIVESYRLPMCPLAP
                     KSRETLAAALKDLRIL"
     gene            complement(1458..2219)
                     /gene="dapB"
                     /locus_tag="VFH83_02255"
     CDS             complement(1458..2219)
                     /gene="dapB"
                     /locus_tag="VFH83_02255"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET6485211.1"
                     /translation="MKALIIGYGSMGREVERVLLDRGHSVAGKIDPAQPGATATALTA
                     ELARGVDVALEFSHAEAVLGNVRIYAEAGLSAVVGTTGWYGKLDEVKGIVGSRIGFLY
                     GSNFAIGAHLFFALAAAATELSNSCPEYDILGWEVHHKRKKDSPSGTALTIAKIITGA
                     SNRKKKVVTERLDRAPAPDELHFASVRGGEVPGTHTVVLDSLFDSIELTHRARGRGGL
                     ALGAVRGAEWLAGRRGVFDVSDFIRDILGGGRSQA"
     gene            complement(2216..3229)
                     /gene="pta"
                     /locus_tag="VFH83_02260"
     CDS             complement(2216..3229)
                     /gene="pta"
                     /locus_tag="VFH83_02260"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET6485212.1"
                     /translation="MNFIDEMRRKAQSDPKRLVLPEGYETRTLKASRIILDGGFAKEV
                     FLLGSRPQIEAEARKQGVNLTGLQIEDPSTSPLRSQYIQSYFELRKHKGISASDAEKT
                     FMGDTQHWGAMMMALGRADAMVSGADSETAKVLRAALTIVKTSPGIKTASSCFVMYLP
                     NTTWGVDGHLIFADCAVVPDPTPEQLADIAVSSAQSCRVFLKADPRVAMLSFSTKGSA
                     THPLVDKVVEALKIVHQKAPDLVADGEMQADAALVASVAAKKAPGSNVAGKANTLIFP
                     DLQAGNIGYKLVQRFAKAEAYGPFLQGFAKPVSDLSRGCSVDDVVNTSVVTLVQSQGK
                     GNG"
     gene            3297..4031
                     /locus_tag="VFH83_02265"
     CDS             3297..4031
                     /locus_tag="VFH83_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HET6485213.1"
                     /translation="MTVGVYGLGRFGSLWAGLLARSFPVCGYTRTADRPTPQGVRRVG
                     EDEVLSQDVLFLCVAISSLEEILQKARARIPPNTLVMDTCSVKSYPVRLMEQHLPEAV
                     GILGTHPMFGPDSARNGIQGLPMILCPCRVAPRLVEQWRSYFLSIGLSVTLMTPEEHD
                     REAAFTQGVTHYIGRVLSDLGVRRSPIGTMGFNKLLEIVEQTCNDPWQLFLDLQRYNP
                     YTGEMRTRLAKSLQSILAAIDGTLSP"
     gene            4127..4756
                     /locus_tag="VFH83_02270"
     CDS             4127..4756
                     /locus_tag="VFH83_02270"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET6485214.1"
                     /translation="MAEIRIKDIDEHEIDTILAEDIDFEGHLTFKKPLMIKGKFKGEI
                     KSTSSLYIGEKAYVEAKTEAGIVSSKGRHRGDIVGHARVELFSTAKVDGDITTPDFVM
                     ESGCKFNGYCNMSGQARKETQPHPQAASSPNAQPVHPPPQNQQQSQQPQSQPQNQQQQ
                     SQQPQAHPAPQAHPAPQGQQGPGGQQSPVNQQGTGNPQGGPGNQQGPQK"
     gene            4753..5391
                     /locus_tag="VFH83_02275"
     CDS             4753..5391
                     /locus_tag="VFH83_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6485215.1"
                     /translation="MTRQLITRLVGLGLILACLAPAFAQDKPDALELYRANRFADAIK
                     VCQQELQDSPRNIDSYVVMGWSQLRLQAYQDALASGQKALTLSPNDPRVVQIVGEAQV
                     FLGQFDVALQNLQQYVALRPGGDRIARVYWLMGECYIRLKQYQNADISISTAVYYEQS
                     NALWWARLGYARELANDLQWASDAYSRALKLDPTLADAQRGKQSVDKKLGGG"
     gene            5388..>6164
                     /locus_tag="VFH83_02280"
     CDS             5388..>6164
                     /locus_tag="VFH83_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HET6485216.1"
                     /translation="MRIGFYTFGCKLNQFETDALVSALRGKGCLIAKEDQPADAYVVN
                     TCTVTARADHKARALVRSLARRHPHSTIVVTGCSAQVEARRLAALADNVVVVPQDRKD
                     RLVELPRLLASGRAPSALLQEIDDAVPPGPFALPAASYGFHARAYLKIQDGCDCSCAY
                     CRVPMARGRSVCLDAEEAIRRAAYLEASGFREIVLTGVNVSSYRFRGMSLGGLLRCVL
                     SATDKARLRVSSLEPEAVSPQLAAELAHPRVCPHFHLPVQS"
BASE COUNT         1047 a         2087 c         1983 g         1047 t
ORIGIN      
        1 accggttccg ctcggagccg cggctcaccc tgatctgcgg ggacttcctg gacacctggg
       61 agaacgccgt tcgggatcat gggcgtccga gcaagatcct gggaaacctg ccctacagct
      121 cggcctcgca gatggtggcg cagatccttg agtccgggat ccaaccgcaa cggctcctgg
      181 ttacggtcca gagggagctg ggggaccgca tggcctcacc gcccagcacc aagagctact
      241 ccgccttctc ggtcctctgt caggcctgct gtgaagtgat cgtccgaggc gagctcaacc
      301 ccgggagctt ctatccaccg ccgaacgtcg tttcggccat cgtcgagctg cgccccaggc
      361 aggacgcgcc gtccggtccc gccttgggcc tgctcacgcg aatcacccgc gcgctcttcg
      421 cgtctcggcg gaagacgctc aggaacaaca tcggccgagc cggactcgac accgggcgcc
      481 tgctcgaggc tctggacgtg ctgggcatcg acggaggcgc tcgggccgaa gagctttcac
      541 caggggacta cgttcggctc gccgaacggc tggccggtac ccctacagga tccgcaggtc
      601 tttgagcgcg gcggccagag tctcccggct cttgggggcc agcgggcaca tgggaagccg
      661 ataggattcc acgatcagac ccttcatggc catggcggcc ttgatgggaa tgggattggt
      721 ctcgacgaac agggccttga agagcggtag aagctcgtag tgcatgctgc gcgccgcggc
      781 ccagtcctcc ttcagcatgg cgttcacaaa ccgcgccatc cgatccggca cgaggttgct
      841 ggccacggag atcacgccgg ttccgcccag tgccatgatg ggaagcacca ggttgtcgtc
      901 tccggagagg accgagaacg cgccgggctt gcggaggatc aactccatga cctgggggat
      961 gtccccgctc gcttccttca ccgccacgat gtttcggtgc cgggccagct ccagcatggt
     1021 tggattgtcg atgttcttcc cggtgcgccc cgcgatgttg tacaccacca ggggcaggtc
     1081 caccgcgtcc gcgatggccc ggaaatgtcg gaggaacccg tcactcgtcg gcttgttgta
     1141 gtaaggagcc acctggagcg tggcaacggc tccgacttcc ttcgcgtccc gggtgtactc
     1201 gatggcctcc gcggtcgcgt tcgagcccgc accggcaatg accggcaccc ggccccggga
     1261 ctgctccaca acgatctgga tgacccgctt gcactcatcg ccgtccagcg tcgggctctc
     1321 cccggtcgtc ccgacgggca cgagaccctg aacgccgccc tcgatctgga aatccacgag
     1381 ccgccgaagc gccttctcgt ccaggctccc gtcgctcgcc atcggggtca ccagcgccgt
     1441 gtaaacgccc tccagcttca tgcctggctc cttccgccgc ccagaatatc ccggatgaaa
     1501 tcgctcacgt cgaagactcc gcgccgaccg gccagccact ccgcgcctcg gaccgcgccg
     1561 agagccagac cgccccgtcc gcgcgcgcgg tgggtgagct ctatgctgtc gaacaaggaa
     1621 tccagcacga cggtgtgcgt gccgggaacc tcgccgccgc gcacggaggc gaagtgcagc
     1681 tcgtccggcg cgggcgcgcg atccaaccgc tccgtgacca ccttcttctt gcggttgctc
     1741 gcgccggtga tgatcttggc gatcgtcagc gccgtccccg acggcgaatc cttctttcgc
     1801 ttgtggtgca cttcccagcc caggatgtcg tactccgggc aggagttgga cagctcggtg
     1861 gcagccgcgg ccagggcgaa gaacaggtgg gctcctatgg cgaagttgct tccatagagg
     1921 aagccgatcc gcgatccgac gatccctttg acctcgtcga gcttgccgta ccagcccgtg
     1981 gttcccacga cggcgctcag cccggcctcc gcgtagatcc ggacgttccc caggaccgcc
     2041 tcggcatggg agaactcgag cgccacgtcc acgccgcgcg ccaactcggc ggtcaaagcc
     2101 gttgccgtgg cccccggctg ggccgggtcg atcttcccgg ctacggaatg cccgcgatcc
     2161 aggagcacac gctccacctc gcggcccatg cttccgtagc cgatgatgag agccttcatc
     2221 cgtttccctt cccctgggac tggaccagcg tgaccaccga cgtgttcacc acgtcgtcca
     2281 cggaacagcc gcgggagagg tcactgacgg gcttggcgaa cccctggagg aaggggccat
     2341 acgcctcggc tttggcgaag cgctgaacca gcttgtaccc gatgttcccc gcctggagat
     2401 ccgggaagat cagcgtgttg gccttgccgg ccacgttgga tcccggagcc ttcttggcgg
     2461 ccacgctggc caccagggca gcgtcggcct gcatctcgcc gtccgccacg aggtccggcg
     2521 ccttctgatg gacgatcttg agcgcctcca cgaccttgtc caccaggggg tgcgtcgcgc
     2581 ttcccttggt ggaaaacgac agcatggcta cccgtgggtc cgccttgagg aagacgcggc
     2641 acgactgggc cgaagagacg gcgatgtccg cgagctgctc cggcgtgggg tcggggacca
     2701 cggcgcagtc cgcgaagatg agatgtccat ccacgcccca agtggtgttc ggcagataca
     2761 tgacgaagca ggaggatgcc gtcttgatgc ccgggctcgt cttgacgatg gtcagggccg
     2821 cgcgcaggac cttcgcggtc tcactgtcgg ccccggagac cattgcgtcc gcccggccca
     2881 acgccatcat catggcgccc cagtgctgcg tatcgcccat gaaagtcttc tccgcgtcgg
     2941 aggcggagat gcccttgtgc ttgcgaagct cgaaatagga ctggatgtac tgcgagcgaa
     3001 gcggggacgt cgaggggtcc tcgatctgca gcccggtgag gttcacgccc tgcttgcgcg
     3061 cctcggcctc gatctggggc cgggagccga gcagaaagac ctccttggcg aaccctccgt
     3121 ccaggatgat gcggctggcc ttcagcgtgc gcgtctcgta gccctccggc aggacgagtc
     3181 tcttggggtc tgactgggcc tttcggcgca tctcgtcgat aaagttcatc ctgggagcct
     3241 cccttcgaca ggttcggagt atagcttttt ccctggaaac tgcaataatc gccgccatga
     3301 ccgttggggt ttacggcttg ggaaggttcg gttcgctgtg ggccggactg ctcgcgcgat
     3361 cgtttcccgt atgcgggtac actcgtacgg cggatcggcc gaccccgcaa ggggtccgaa
     3421 gggtggggga ggacgaggtg ctctcccagg acgtgctgtt cctctgcgtc gccatatcgt
     3481 ccctggagga gatcctgcag aaggcacgcg cccgcatccc gccgaacacg ctggtgatgg
     3541 acacctgctc cgtcaagagc tacccggtgc gcctcatgga gcagcatctc cccgaggcgg
     3601 tgggcatcct cggcacgcac cccatgttcg gtccggactc cgcccgaaac ggcatccagg
     3661 ggctccccat gatcctctgc ccgtgccgtg tggccccgcg cctggtggag cagtggaggt
     3721 cctacttcct gtccatcggt ctgtccgtga ccctcatgac tccggaggag cacgaccgcg
     3781 aggccgcgtt cacccagggg gtcacgcact acatcgggcg agtcctgagt gacctgggcg
     3841 ttcgccgctc tcccatcggc acgatgggct tcaacaagct cctggagatc gtggagcaga
     3901 cctgcaacga tccgtggcag ctcttcctcg atctgcagcg ctacaaccct tacacgggag
     3961 agatgcggac ccgcctggca aaatcgctcc agagcatcct cgcggcgatc gacggaaccc
     4021 tttcgccttg acatcggccc ggcccggaag gtacgctccc ggagtgtgtg ggcggtggac
     4081 aacggccccc ggcgcgcgca tgcgcgccgc aaggagatga acggaaatgg cggagattcg
     4141 catcaaggac atcgacgagc acgagataga caccatcctg gcggaagaca tcgatttcga
     4201 gggtcatctc acgttcaaga agcccctgat gatcaagggc aagttcaagg gagagatcaa
     4261 gtccaccagc tccctctaca tcggggagaa ggcctacgtg gaggccaaga ccgaggcggg
     4321 catcgtctcg tccaagggcc ggcatcgggg agacatcgtc ggtcacgcgc gggtcgagct
     4381 cttctccacg gcgaaggtgg atggtgatat caccacgccg gacttcgtga tggagagcgg
     4441 ctgcaagttc aacggctact gcaacatgag cgggcaggcc cgcaaggaga cgcagcctca
     4501 cccgcaggcc gcctcctcgc cgaatgctca gccggttcac ccgccgccgc agaaccagca
     4561 gcagagccag cagccgcaga gccagccgca gaaccagcag cagcagagcc agcagccgca
     4621 agcgcaccca gccccgcagg cacatccggc tccgcagggg cagcaagggc cgggcggcca
     4681 gcagagtccc gtcaaccaac aaggtaccgg caatccgcag ggcgggccgg ggaaccagca
     4741 ggggccgcag aaatgacgcg acagctcatc acccgccttg tcggcctggg tctgatcctc
     4801 gcctgcctgg cgccggcgtt cgcccaggac aaaccggacg cgctggagtt gtaccgggcc
     4861 aaccggtttg cggacgccat caaggtctgc cagcaggagc tgcaggactc gcctcgcaat
     4921 atcgactcct acgtggtgat ggggtggagc cagcttcggc tccaggccta ccaggatgcc
     4981 cttgccagcg ggcagaaggc gctgaccctc agcccgaacg atccgcgggt cgtccagatc
     5041 gtcggcgaag cgcaggtgtt cctgggtcag ttcgatgtgg ccctccagaa ccttcaacag
     5101 tacgtggcgc tgcgcccggg cggagaccgg attgcccgtg tctactggct catgggggaa
     5161 tgctacatcc gactgaagca gtaccagaac gccgacatct cgatatccac cgccgtgtac
     5221 tatgagcaga gcaacgctct gtggtgggcc cgcctggggt atgcccggga gctcgccaac
     5281 gacctgcagt gggcgtcgga cgcatactcc cgcgcgttga agctcgaccc cacgctggcg
     5341 gacgcccaga ggggcaagca gagcgtggac aagaagctcg gcggcggatg aggatcgggt
     5401 tctacacctt cggctgcaag ctcaaccagt tcgagaccga cgcgcttgtt tccgcgctgc
     5461 ggggcaaggg ctgcctcatc gccaaggagg atcagcccgc ggatgcctac gtggtcaaca
     5521 cctgcacggt gaccgcgaga gcggaccaca aggcgcgggc tctggtacgc tccctggctc
     5581 ggaggcaccc ccattctacc atcgtagtca ccggctgctc ggcccaggtg gaagccagaa
     5641 ggctggcagc gcttgcggac aacgtggtgg tcgtaccgca ggatcgaaag gaccgcctcg
     5701 tcgagctccc gcggctcctg gcttcgggcc gcgcgccgtc ggctctgctc caggagatcg
     5761 acgatgccgt cccgccgggt cctttcgcgc ttcccgccgc cagctacggc ttccacgcgc
     5821 gagcgtacct gaagatccag gatggctgtg actgctcctg cgcttattgc cgggtcccca
     5881 tggcgcgggg acgctcggtg tgcctggacg cggaggaggc gatccgccgc gccgcttatc
     5941 tcgaagcatc cgggtttcga gagatcgttc tcacgggtgt gaacgtctcc agctaccggt
     6001 tccgggggat gagccttggc gggctcctgc ggtgcgtgct gtcggccacc gacaaggcgc
     6061 ggcttcgcgt ctcctcgctc gagccggagg ccgtgagccc gcagctcgcc gcagagctcg
     6121 ctcatccccg cgtttgtccc cacttccacc tgccggtcca gtcg
//
LOCUS       DASRZS010000093         4307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5052864, whole genome shotgun sequence.
ACCESSION   DASRZS010000093 DASRZS010000000
VERSION     DASRZS010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5052864"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFH83_02285"
     CDS             <1..334
                     /locus_tag="VFH83_02285"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HET6485217.1"
                     /translation="EGVLGKYPVYILTLKAKNDEVSYPMMKLSVRKDKPLALKEEDYS
                     LSNRLMRTVLYPPKYIEVAGRTVYSQMLIEDDLNVGERSALSIADVSVARLPDATFSK
                     AFLDQSSK"
     gene            351..1862
                     /locus_tag="VFH83_02290"
     CDS             351..1862
                     /locus_tag="VFH83_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485218.1"
                     /translation="MHRRSVLVASVLVGAAVAVGPVAAQDDVFNSDESTTTTTDQTQN
                     ATPRNDILKEAVPRLTGTFQGTAGAAWNWSDVWNSPFNILEPSSSSLNQQATELDLGF
                     VARPDTDISFTGEIRTTYPFVDQITTGSGATASTLLVPDFTVWSLYSKFTWNDSLFFT
                     FGKQPLKWGTGYFFSPADDVFAQTAVDVTNPTAERQGPLALKVQYPIPKTQDNLYFIA
                     ALPAANNLTTLASLQPQDIAVAAKAEFLFGNTEVAGAGYYQLQQRPRAILMATTGTGN
                     LNFFGEGLAAFPSSQSDPYIEKTAVTWTIGALPRTSDYSVVDRSADTFYKATAGVMYT
                     NQDWNFTAVAQYLYNGQGYDTLSVQDILQAVIDRALGQPAGEPTLNTATLASDFTGLG
                     QIGVHYGVIYLGFTSIANTKTDFSVLGIMNLSDGSGYVNPTVSFTMFTYVKLSLGASA
                     SWGAAGTEFADPTGFASTFPTINGQPNPSYNPNYVAKPTFALTLNASVGTGSF"
     gene            2060..3061
                     /locus_tag="VFH83_02295"
     CDS             2060..3061
                     /locus_tag="VFH83_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HET6485219.1"
                     /translation="MKPPLAEGKLPPELLGRLLAYTSHAPELLVGAQPGEDAAVAAGA
                     PTLVLTTDPITFTADRIGLYVVAVNANDIVAMGGRPIYLTTTLLLAPGATEDDVERVF
                     VDIAAACRRAGMLWVGGHTEVTPVVTRTVVCGQAVGFLSGPPLSTGGARVGDLLCMSK
                     WVGLEGTATIAQERPRETQEVLGAARYREVLGWVESPGICIVEEGKALEGMALSAGHD
                     PTEGGIAMGIQEMCQRSGLGARVQASALPIREETRLLCERYGLDPTGLLSSGVFLFAA
                     PPKVAQEACRILQARGIPACSIGEMLGPGEGVWLERDGARTPLRFSAQDEIVKLGRA"
     gene            complement(3058..>4307)
                     /locus_tag="VFH83_02300"
     CDS             complement(3058..>4307)
                     /locus_tag="VFH83_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6485220.1"
                     /translation="IPPDREYGFNVSYDKSTGYRSKSMLTVPMKDHKGEILGVVQLIN
                     RKENNDVRMPTPQDVERLAIPFSPEQEPLVLSLASQAAVSLENNLLYQEIETLFEGFV
                     QASVQAIESRDPTTSGHSNRVAVYTVGLARAVDNAGIGAYKDVHFSAEHLKEIRYASL
                     LHDFGKVGVREHVLVKAKKLYEPQLELLKMRLRYIEKSTLYALLKKRFEAICSEGMQG
                     YERLKTDIDREEREALAEIDRYLKAIVSANEPTVLNEEPARILDEIYGKSFEEEGEDP
                     IPILTADEYVKLKIRKGSLDEAERKEIESHVTHTFMFLRRIPWTKEMRNIPTIAYGHH
                     EKLDGAGYPRRIQATEIPIQTRMMTVSDIFDALTASDRPYKPAVPARKALDILSEDVQ
                     NNHLDADLVRVFIDARIWEKKES"
BASE COUNT          731 a         1432 c         1388 g          756 t
ORIGIN      
        1 ggagggggtg ctgggcaagt accccgtcta catcctcacg ctcaaggcga agaacgacga
       61 ggtgagctat cccatgatga agctctccgt gcgcaaggac aagcctctgg cgctcaagga
      121 ggaggactac agcctctcca accggctcat gcgcaccgtg ctctaccccc ccaagtacat
      181 cgaggtggcc gggcggaccg tgtactccca gatgctcatc gaggacgatc tgaacgtcgg
      241 ggagcgcagc gccctttcga tcgcggacgt atcggtggcg cgccttccgg atgccacgtt
      301 ttccaaagcg tttctggacc agtcctcgaa gtaggggaga ggggggaacc atgcatcgca
      361 gatccgtact ggtcgcctcc gtcctggtcg gagccgccgt cgccgtgggg ccggtagcgg
      421 cgcaggacga cgtgttcaac tccgatgaga gcacgaccac caccacggac cagacgcaga
      481 acgcgactcc acgcaacgac atcctgaagg aggcggtgcc gcgtctcacc ggcaccttcc
      541 agggcaccgc cggcgccgcc tggaactgga gcgacgtctg gaactcgccc ttcaacatcc
      601 tggagccctc cagcagcagc ctgaaccagc aggcaacgga gctggacctg gggttcgtgg
      661 cgcggccgga cacggacatc agcttcaccg gcgagatccg gaccacctac cccttcgtgg
      721 atcagatcac cacggggagt ggagccacgg cgtccaccct cctggtgccg gacttcacgg
      781 tctggtcgct gtactccaag ttcacgtgga acgactcgct tttcttcacc ttcgggaagc
      841 agcctctgaa gtggggcacc gggtacttct tcagccccgc cgacgacgtc ttcgcgcaga
      901 ccgcggtgga cgtgaccaac cccacggcgg agcgccaggg gccgcttgca ctcaaggtcc
      961 agtaccccat ccccaagacc caggacaacc tctacttcat tgcggcgctt ccggccgcca
     1021 acaacctgac cacgctcgcg tcgctgcagc cccaggacat cgccgttgcc gcgaaggccg
     1081 agttcctctt cggcaacacc gaggtggcgg gagccggcta ctaccagctc cagcagcggc
     1141 cacgggccat cctcatggcc accacgggca cgggcaacct gaacttcttc ggggaaggcc
     1201 tggccgcgtt ccccagctcg cagagtgacc cgtacatcga gaagacggcg gtcacctgga
     1261 cgattggcgc gttaccccgt acgtcggact acagcgtcgt ggatcgaagc gccgatacct
     1321 tctacaaagc gacggccggg gtgatgtaca cgaaccagga ctggaacttc accgcggtgg
     1381 cgcaatacct ctacaacggc caggggtacg ataccctgag cgtgcaggac atcctgcagg
     1441 cggtgatcga tcgcgcgctg ggacaacccg cgggggagcc gaccttgaac accgcgaccc
     1501 tggccagcga cttcaccggg ctggggcaga tcggcgtgca ctacggggtc atctacctgg
     1561 gctttacctc catcgccaac accaagacgg acttctccgt cctggggatc atgaacctgt
     1621 ccgatggatc ggggtacgtg aacccgaccg tgtccttcac gatgttcacg tacgtcaagc
     1681 tttccctggg ggccagcgcc tcctggggcg cggcggggac cgagttcgcc gatcccacgg
     1741 ggttcgccag cacgttccct accatcaacg gccagccgaa ccccagctac aacccgaact
     1801 atgtcgccaa gccgaccttc gcgctcacgc tgaacgccag cgtgggaacg ggatcgttct
     1861 agggcgcggc gcgagcgtcg gccgcggacc cggaatcccg gggcggcacc ttccaccctc
     1921 tcgaaatcga cggaatcgca agctgcgctt tcgtggtaat cgagagggtg ggaaagcgct
     1981 gccgggaaaa ccggtcgacc ctgccggaaa aaccgcgcct gttgcccatc gctccgcctt
     2041 ccggcatcat cgagtcatca tgaaaccacc gctggcggag gggaagcttc cgccggaact
     2101 gctgggccgc ctgctggcct acacttccca cgcgcccgag ctgctggtcg gcgcgcagcc
     2161 gggagaggac gcggccgtgg ccgccggcgc gcccaccctc gtgctgacca cggatcccat
     2221 cacgttcacc gcggatcgaa tcggtctcta cgtggtggcg gtgaacgcca acgacatcgt
     2281 ggccatgggc ggccggccga tctacctcac caccacgctt ctcctggcgc ccggcgccac
     2341 ggaggacgac gtcgagcgcg tcttcgtgga catcgcggcc gcctgtcgac gggcgggaat
     2401 gctatgggtc ggcgggcaca ccgaggtcac gcccgtggtc acgcggaccg tggtctgcgg
     2461 ccaggcggtg gggttcctct ccggtcctcc gctctccacc ggcggcgcca gggtgggaga
     2521 tctcctgtgc atgagcaagt gggtgggtct ggagggcacg gccacgatcg cccaggagcg
     2581 tcctcgggaa acccaggagg tcctgggcgc tgcacggtat cgggaagtcc tgggatgggt
     2641 ggaaagccca ggcatctgca tcgtcgaaga aggcaaggcc ctggaaggga tggccctctc
     2701 ggccggccac gatcccaccg agggcgggat cgccatggga atccaggaga tgtgccaacg
     2761 aagcggtctg ggcgcccggg tccaggcgtc cgccttgccc atccgcgaag agacccgtct
     2821 gctctgcgaa cgctacgggc tggaccccac cggcctcttg agctcgggcg tgttcctctt
     2881 tgccgcgcct cccaaagttg cccaagaggc ctgccgcatc ctccaggcgc gcggaatccc
     2941 cgcgtgctcc atcggagaga tgctcggccc gggtgaggga gtgtggctgg agcgggacgg
     3001 cgcgcggact ccgttgcggt tctcagccca ggatgagatc gtcaagctgg gacgagccta
     3061 gctttccttc ttctcccaga tcctggcgtc gatgaacacg cgcacgaggt ccgcgtccag
     3121 gtggttgttc tgcacgtcct ccgagaggat gtccagcgcc ttgcgcgcgg gtacggcggg
     3181 cttgtagggc cggtcggacg cggtgagcgc gtcaaagata tcggaaacgg tcatcatgcg
     3241 ggtctggatg ggaatctcgg tcgcctggat cctgcggggg tacccggcgc cgtccagctt
     3301 ctcgtggtgg ccgtaggcga tggtggggat gttgcgcatc tccttggtcc acgggatccg
     3361 ccgcaggaac atgaaggtgt gggtgacgtg gctctcgatc tccttgcgct ccgcctcgtc
     3421 caggctgccc ttgcggatct tgagcttcac gtattcgtcc gcggtcagga tcgggatggg
     3481 atcctctccc tcctcctcga agctctttcc gtagatctca tccaggatcc gcgccggctc
     3541 ctcgttgagc accgtcggct cgttcgctga gacgatggcc ttgaggtagc ggtcgatctc
     3601 cgccagcgcc tctcgctctt cgcggtcgat gtccgtcttg agcctctcgt atccctgcat
     3661 cccctcggag cagatcgcct cgaagcgctt cttgagcagg gcgtacagcg tcgacttctc
     3721 gatgtagcgc agccgcatct tcaggagctc gagctgcggc tcgtagagct tcttggcctt
     3781 gaccaggacg tgctcgcgaa cgcctacctt gccgaaatcg tggagcaggc tggcgtagcg
     3841 gatctccttg aggtgctcgg cgctgaagtg gacgtccttg tacgccccaa tgcccgcgtt
     3901 gtccacggcg cgcgccagac ccaccgtgta gaccgccacg cggttggagt gaccggaggt
     3961 ggtgggatct cgggactcga tggcctgcac ggatgcctgg acgaaccctt cgaagagcgt
     4021 ctcgatctcc tggtagagca ggttgttctc cagggacacg gccgcctggg acgccaggga
     4081 gaggaccagg ggctcctgct ccgggctgaa gggaatggcc agccgctcca cgtcctgcgg
     4141 cgtcggcatg cggacgtcgt tgttctcctt gcggttgatg agctgcacca cacccaggat
     4201 ctcgcccttg tggtccttca tgggcacggt gagcatgctc ttggaccgat agcccgtgga
     4261 cttgtcgtag ctgacgttga acccgtactc ccgatccggc gggatct
//
LOCUS       DASRZS010000094         3228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3511892, whole genome shotgun sequence.
ACCESSION   DASRZS010000094 DASRZS010000000
VERSION     DASRZS010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3511892"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="VFH83_02305"
     CDS             complement(<1..1363)
                     /locus_tag="VFH83_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485221.1"
                     /translation="MRPRLWPWITIWAACLALVVSLYVVYSRLPADGMTGDMSSYSSQ
                     GFRLLWVLEPRSDGLRVNDVVVRAGGHSVDEWLSGAPRGPEWNTGGVVTYDVLRDGVL
                     LSIPVRMAPVSLLSIVRRWELQLIAAAGFLLIGTFVFLKRIRELPARILMLFCVFTCM
                     QYLGDGFNFQYAIMPLRWLFWAHFLWENLTYDIALSAACYFAFLYPKVNPLVGKVPGL
                     VPAFLFAVLPAELLISMLAAPGWSHGIAVANQVSWIFLIVMVPLALAAALHSIRTAKD
                     PVSRAQMRWILWSVSVGGVAATLLYVVPLILDVRPILPHPFVMLGLAGLALIFAIGIL
                     RFRLFEIDIIINRTLVYGSLTVVLMALYLVLVQLLSAFAEVVLKRQNDTVVLFLATLC
                     IALAFAPLRQRVQLLIDRAFFRTRLDFQRLLPEMTEELAGSILVEQLAATLQRRLPQA
                     LQIS"
     gene            complement(1360..2262)
                     /locus_tag="VFH83_02310"
     CDS             complement(1360..2262)
                     /locus_tag="VFH83_02310"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HET6485222.1"
                     /translation="MRTVLPALALLLCLCAGAAADVASLGASAGAEPRPSALTVVIDD
                     FERFTPPIHGSTLRSDAWSVIYDKGRTTIDTVSEAAPALTAGSSRVLRIHGESNSYDI
                     AHEVPFHWISQYPVLRWKWKVTQFPVGADISDLSRDDSAAQIYVNFDLKASFLFYPDL
                     LSICYYYGSTEPDGHVFLWEGYGTFVEFICIRTVPKDGIGVWFAEERDVLRDYRKVIQ
                     DFSTDPDTGMRAKFLKAYAHALGAPPRADPARDPELEVHSVALWVDSNDTRSTAESLW
                     DDITFSAPAPGSSSGPEEASESPR"
     gene            2355..3080
                     /locus_tag="VFH83_02315"
     CDS             2355..3080
                     /locus_tag="VFH83_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6485223.1"
                     /translation="MELKGKVILVVGGATGIGRAAAVLCARRDARVIVGDRNRTEGGQ
                     AAAECGGTYIPVDVTDEGSVQAMAASIDQRFGRLDALIHTAGILQGAYVPLEEFSLAT
                     WKSVLEVNTTGSFLCARTVVPLMKKAGKGVIVLVSSGAAVGGSSSFAYGTSKGGVNSL
                     GIVLANALASQNIRVNVVAPGNIDTAMKRSVIAADVDRRGMGDLERAITDSHLGAPEG
                     VARLLAWLVSDDADYVRGLINTR"
BASE COUNT          619 a         1007 c         1128 g          474 t
ORIGIN      
        1 acgagatctg cagggcctgc gggagcctcc gctggagcgt ggcggcaagc tgctccacca
       61 ggatgctgcc ggccagctcc tcggtcatct cgggcagaag ccgctggaag tccagccggg
      121 tccggaaaaa cgcccggtcg atgagaagct gcacccgctg ccgcagcggc gcaaaggcca
      181 gcgctatgca caacgtggcc aggaagagga ccacggtgtc gttctgccgt ttgaggacca
      241 cttcggcgaa cgcgctgagg agctggacca ggaccaggta cagcgccatg agcacgaccg
      301 tgagcgagcc atagaccagc gtgcggttga tgatgatgtc gatctcgaac agccggaacc
      361 gcaggattcc gatggcgaag atcagcgcga ggcccgccag gcccagcatc acgaacggat
      421 gcggaaggat cggccggacg tccaggatca ggggcaccac gtacagcaag gtggctgcga
      481 cgccgcccac ggagacgctc cagaggatcc accgcatctg ggctcgggag accggatcct
      541 tcgccgtgcg gatggagtgc agcgcggccg ccagggccag cggcaccatc acgatcagga
      601 agatccagga cacctggttg gccaccgcga tcccgtggct ccatccggga gccgcaagca
      661 tgctgatgag cagctccgcg ggcaggacgg caaagaggaa cgccggcacg aggccgggga
      721 ccttccccac cagggggttc acctttggat agaggaacgc gaagtagcag gccgccgaga
      781 gggcgatgtc gtaggtgagg ttctcccaga ggaaatgggc ccagaacagc catcgcagcg
      841 gcatgatggc gtactggaag ttgaatccat cccccaggta ctgcatgcag gtgaacacgc
      901 agaagagcat caggatgcgc gccgggagct ctcggatacg cttgaggaag acaaaggtcc
      961 cgatcagcag gaaaccagcg gcggcgatga gctgcagctc ccaacggcgg acgatcgaaa
     1021 ggagcgaaac gggcgccatt cggaccggga tgctcaggag gacgccgtcc cgcaggacgt
     1081 cgtaggtgac caccccgccg gtgttccact ccggaccgcg aggcgcgccg gagagccact
     1141 cgtccacgga gtggccgccg gccctcacca ccacgtcgtt gacgcgcaga ccgtcgcttc
     1201 gcggctccag cacccagaga agccgaaagc cctgggagct gtagctgctc atgtctccgg
     1261 tcattccgtc cgcgggcagc cgggagtaca cgacgtaaag ggagacgacc agcgccaggc
     1321 acgcggccca gatggtgatc cacggccaga gtcgtggcct catcgtgggc tctccgaggc
     1381 ctcttcgggc ccggaggagg agcccggcgc gggagcgctg aaggtgatgt catcccacag
     1441 gctctcggcc gtggagcggg tgtcgttgga gtccacccag agcgccaccg agtggacctc
     1501 gagctccggg tcacgcgcgg gatcagctct gggcggcgcg cccagggcgt gagcgtacgc
     1561 tttcaggaac ttggcgcgca tgccggtatc cgggtccgtg gagaaatcct ggatgacctt
     1621 gcggtagtcc cgaagaacgt cccgctcctc ggcgaaccag acgccgatgc cgtccttcgg
     1681 gaccgttcgg atgcagatga actccacgaa cgtaccgtaa ccctcccaga ggaagacgtg
     1741 gccatccggc tccgtggagc catagtagta gcagatggac agaagatcgg ggtagaagag
     1801 gaatgaggcc ttgagatcga agttgacgta gatctgggcg gcgctgtcgt ccctggacag
     1861 gtcggagata tccgccccca ccggaaactg cgtcaccttc cacttccagc gcaggacggg
     1921 gtactggctg atccagtgga aggggacctc gtgggcgata tcgtaggagt tgctttctcc
     1981 gtggatccgc aggacgcgcg aggagcccgc ggtgagggcc ggcgcggcct cgctcaccgt
     2041 gtcgatcgtc gtcctgcctt tgtcgtagat gaccgaccag gcgtcggatc ggagggtcga
     2101 cccgtggatg ggtggggtga agcgctcgaa gtcgtcgatg accaccgtga gagccgacgg
     2161 tcggggctcc gcgccggcgg atgcgcccag ggatgcaacg tccgctgcgg cgcccgcgca
     2221 gaggcaaagg aggagcgcca gcgccggcaa cacggttctc atccctacag agtctatccc
     2281 ggaaggagcg ccggcgcaaa ccagtatgta tcacacgtag attccgcacc gcggatccgt
     2341 taggattccc cgacatggag ctcaaaggga aagtcatcct ggtggttggc ggcgccacgg
     2401 ggatcggtcg cgccgccgcg gtcctgtgcg cccgacggga tgcccgggtc atcgtggggg
     2461 accgcaaccg gaccgaaggg ggccaggctg ccgccgaatg cggcgggacc tacatccccg
     2521 tggacgtgac ggacgagggc tcggtccagg ccatggcggc gagcatcgac caacgcttcg
     2581 gccgcctgga cgcgctcatc catacggccg gaatcctgca gggggcatac gtgccgctgg
     2641 aggagttctc cctggccaca tggaagtccg tcctggaggt caataccacg gggagcttcc
     2701 tgtgcgcccg gacagtcgtc cctctgatga aaaaggcggg caagggcgtc atcgtgctgg
     2761 tctcttctgg cgccgcggta ggcggcagct cctccttcgc ctacgggacg agcaagggcg
     2821 gggtcaatag cctgggcatc gtgctggcaa acgcgctggc atcccagaac atacgggtca
     2881 acgtcgtggc accgggcaac atcgacaccg ccatgaagcg ctccgtgatc gcggcggacg
     2941 tggatcgacg cggcatggga gacctggagc gagctatcac cgactcgcac ctgggcgccc
     3001 cggaaggtgt ggcgcggctc ctggcgtggt tggtgagcga cgatgccgac tacgtccgcg
     3061 gcctgatcaa cacccgatga agctgggagt cgcgggcatc gtctcggagt ggacccagat
     3121 cggccccgcg caagcccggc gggtccgcgg ctacgggttc cacggtctct ccgtcttctt
     3181 ctccgatccg ctggccgccc aggaagggaa ggtggtcgag cttcgacg
//
LOCUS       DASRZS010000095         4715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2743962, whole genome shotgun sequence.
ACCESSION   DASRZS010000095 DASRZS010000000
VERSION     DASRZS010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2743962"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFH83_02320"
     CDS             <1..528
                     /locus_tag="VFH83_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6485224.1"
                     /translation="AWAMFSVGDGFVNRLLLSLKAIHTEINFLAADRNVWVTMLGYSL
                     WKNLGWTAILYLASITAIDPGLYEAATIDGAGRFRLIWHITVPGILSTYFVLLLLSIA
                     NFINNGLEQYFVFQNPMNKATIEVLDLYVYNQGMVGQQIPFATAVSMLKSLVSVVLLF
                     VANRMSRFVRGQSIL"
     gene            542..1435
                     /locus_tag="VFH83_02325"
     CDS             542..1435
                     /locus_tag="VFH83_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485225.1"
                     /translation="MKASVGRIAFEALVWVAFLLFTLICVFPFYYIFINTISDNKLAE
                     TGKVLFLPVGIHLQNYVQVARIRGLVHAALVSVARTVLGTAFTLLGSSWLGYCLSKRE
                     MWMRKLSYRFVVVTLYFNAGLIPWFVTMKMLGLVNNFWAYVLPAIVSPFYLILFKTFI
                     ESIPEALEESAQMDGAGYVARYLRIILPLSTSILATIAVFASVGQWNSFVDTLFLMTK
                     SNLFTLQFLLYQYLNEVNSIAALMRTSPQYGQTIDPSKLLNATSIRMTISMVVVIPIL
                     IVYPYFQRFFVKGIMIGAIKG"
     gene            1480..3174
                     /locus_tag="VFH83_02330"
     CDS             1480..3174
                     /locus_tag="VFH83_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6485226.1"
                     /translation="MNGRILLSVALVLCLAAGGSVLAAGKQEAPKPGEVVTLSTFSMP
                     ANKSGLQEGWWADILKEKVGVQIELMPSGDQGEQKLQALMAAGELPDVVVFKDKKQVE
                     DAIRARLLVSLDDNLAKLPNVAKNAATALKYYRDSASAGTGKAYAIPNGIGPGEVGDE
                     INWGPFLRWDLYKKLGMPELSTVEDYLPLLKKMQDMQPTNADGQKVYGISMWKDWDNY
                     TMHQAMQPSVLAGVDTGDQLGSALPFLQVDLSTGATKSILDPSSYYLRALKFYYTANQ
                     MGLVDPDSLTQRFDTALAKAEQGRILFAWWPWFVGNYNKPSNTNADSWSGFRPVLPKD
                     YKAFWWGTNPVGSSWPFAIGASTKHLDAALRYVDFMYSMDGLTLLMNGPKGVTWDLDS
                     NGKPAITANGWDIIENQKDLPAGGKIGDGVAVVNSYGLSGATINPATGVALSFGYWPS
                     SKGRNPTNLLKDWQKTTGYKTTSEMLWARKMYTLTPLAMRMVPVVPDDIQQICSRIGD
                     VVKTTSWQMVFAKNDAEYQSLLADMTSKATGLGLQQVLDWDAQAWQKAQDSAKHYQ"
     gene            3241..>4715
                     /locus_tag="VFH83_02335"
     CDS             3241..>4715
                     /locus_tag="VFH83_02335"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET6485227.1"
                     /translation="MKRRRWMFSNRLMLINALVLIAVLYVFAALAAVSLSRLELSRQL
                     QQRREVLAALFDAYDGKHDEFFSIIFPLYDEKENYDTLSELLERPSDDALMADPFFKQ
                     RVTVMMRGLAIRDPDIVAILLYKNATRGRYVYDIRYQNFEAVSPTYPFFPQLSAKPAG
                     RVVYGARELGSGRDARSVYGIAGTLGTRALRMDAGNIMVAYDAAPFTRILQRNQGSEA
                     DRYLILASGGEAVFDSRGAHPGQSSVYRELLDVGLGNRPRVIDGTPYYVQTVRDPGRT
                     SVGVLLVPRSVVRSAAVGISVTIFAICTAVAALSAGLYLLAGLLVSRRVAEVERAMDR
                     IGSHNLGYRIPLRGRDDEFEHISQRFNRMCDELQRNIDRLYVYQLNQKSAELSALQAR
                     INPHFLYNTLEAIRATFEEEGSGEAGEMIVLLADMFRGITRADPIVTLRDELNTCRTY
                     LDLMTRRFSDGLQVEFAVEEQILELGTVRNLLQPVVENCLL"
BASE COUNT          866 a         1500 c         1460 g          889 t
ORIGIN      
        1 gcgtgggcga tgttctccgt gggagacggc ttcgtgaacc ggctgctcct gtccctgaag
       61 gcgatccaca cggagatcaa cttcctggcc gcggaccgaa acgtctgggt caccatgctg
      121 ggctactcgc tgtggaagaa cctggggtgg accgccatcc tctatctggc gtcgatcacc
      181 gccatcgatc ccgggctcta cgaggcggct accatcgatg gggccggccg gttccgcctc
      241 atctggcaca tcacggtgcc gggaatcctt tccacgtact ttgtgctcct gctcctgtcc
      301 atcgccaact tcatcaacaa cggcctggag cagtacttcg tattccagaa tcccatgaac
      361 aaggccacga tcgaggtgct ggatctctac gtctacaacc agggcatggt ggggcagcag
      421 atccccttcg ccaccgcggt gagcatgctg aaatccctgg tgagcgtggt gctgctgttc
      481 gtggccaatc ggatgtcccg gttcgtccgt gggcaatcca tcctctgaac cggaggccag
      541 ggtgaaggcg tccgttggca ggatcgcgtt cgaggcgctc gtctgggtgg cctttctgct
      601 gttcacgctg atctgcgtgt tccccttcta ctacatcttc atcaacacga tcagcgacaa
      661 caagctggcg gagacgggga aggtcctctt ccttccggtc ggaatccatc tgcagaacta
      721 cgtgcaggta gccaggatcc gaggcctcgt ccatgcggcg ctggtctccg tggcgcgcac
      781 cgtgctggga accgccttca cgctcctggg ctcctcgtgg ttgggatact gcctgagcaa
      841 gcgcgagatg tggatgcgca agctctccta ccggttcgtc gtggtcacct tgtacttcaa
      901 cgcggggctc atcccgtggt ttgtcaccat gaagatgctg gggctggtca acaatttctg
      961 ggcctacgtg ctccccgcca tcgtctcacc gttctacctg atcctgttca agacgttcat
     1021 cgagtcgatc cccgaggcgc tggaggaatc cgcgcagatg gatggcgcgg gctacgttgc
     1081 gcgctacctg cggatcatcc tgccgctctc cacgtcgatc ctcgccacca tcgcggtgtt
     1141 cgccagcgtt ggccagtgga actcgttcgt cgacaccctc ttcctgatga cgaagtccaa
     1201 ccttttcacg ctgcagttcc tgctctacca gtacctgaac gaggtgaact ccatcgcggc
     1261 cctcatgcgt acctctcctc agtacggtca gaccatcgat ccgtccaagc tcctgaacgc
     1321 cacctccatc cgcatgacga tctccatggt ggtggtgatc ccgatcctca tcgtctaccc
     1381 gtatttccag cggttctttg tgaagggcat catgatcgga gccatcaagg gatgaaccta
     1441 gatcgtcgat ctagtcctat ttcgcgtaag gaggatccta tgaacggacg tattcttctg
     1501 tcagtggctt tggtcctctg cctggcggcc ggaggctcgg tcctggccgc gggcaagcag
     1561 gaagccccga agccgggaga ggtcgtcacg ctttccacct tctccatgcc ggcgaacaag
     1621 tccggactgc aagagggctg gtgggccgac atcctgaagg agaaggtggg agtccagatc
     1681 gagctgatgc cgtccgggga ccagggggag cagaagcttc aagccctgat ggcggccggg
     1741 gagcttcctg acgtcgtcgt cttcaaggac aagaagcagg tggaagacgc catccgcgcc
     1801 aggctgctcg tgtccctcga cgacaacctg gccaagctgc ccaacgtggc caagaacgcg
     1861 gcaacggccc tgaagtacta tcgcgacagt gccagcgccg gcaccggcaa ggcgtatgcc
     1921 atcccgaacg ggattgggcc cggcgaggtc ggggacgaga tcaactgggg gccgttcctc
     1981 cgctgggacc tgtacaagaa gctgggcatg ccggagcttt ccaccgtgga ggactatctg
     2041 ccgcttttga agaagatgca ggacatgcag ccgacgaacg cggacggcca gaaggtgtat
     2101 ggcatctcca tgtggaagga ctgggacaac tacacgatgc accaggccat gcagccttcc
     2161 gtcctggccg gcgtcgacac gggggaccag ttggggtcgg ctctgccatt cctccaggtg
     2221 gacctgagca ccggcgccac gaagagcatc ctggatccca gcagctacta cctgcgggcc
     2281 ctgaagttct actacacggc aaaccagatg ggcctcgtgg acccggattc cctgacccag
     2341 cgtttcgata cggcgctggc aaaggccgag cagggtcgca ttctctttgc gtggtggccc
     2401 tggttcgttg gcaactacaa caagccatcc aacacgaacg cggatagctg gagcgggttc
     2461 cgcccggtgc tcccaaagga ctacaaggcg ttctggtggg gcaccaaccc cgtgggctcc
     2521 tcctggccat ttgccatcgg ggcatccacg aagcacctgg acgccgccct tcgctacgta
     2581 gacttcatgt actccatgga cggtctgacg ctgctcatga acggccccaa gggcgttacc
     2641 tgggacctgg actcaaacgg aaagccggcc atcacggcca acgggtggga catcatcgag
     2701 aaccagaagg accttcccgc cggcggcaag atcggtgatg gagtggcggt tgtgaactcc
     2761 tacgggttgt ctggcgccac catcaacccg gcaaccggcg tggcgctgag cttcgggtac
     2821 tggcccagct cgaaaggccg caacccgacg aacctcctga aggactggca gaagacaacc
     2881 ggctacaaga caacgagcga gatgctgtgg gccaggaaga tgtacacctt gaccccgctc
     2941 gccatgcgga tggtgcccgt ggtgcccgat gacatccagc agatctgctc ccgaatcggg
     3001 gacgtggtga agaccacctc ctggcagatg gtcttcgcga agaacgacgc ggaataccag
     3061 agcctgctcg cggacatgac gagcaaggca accggcctgg gactgcagca ggtcctggat
     3121 tgggatgcgc aggcctggca gaaggcccag gattccgcga aacactacca gtagaaaggc
     3181 cgaggcggcg gtcggcggtc cctgcgggcc gccgacccct tgggtctcga gcggtcctcc
     3241 atgaagcgac gccggtggat gttctccaac cgactcatgc tgatcaacgc cctggtgctc
     3301 atcgcggtcc tctatgtctt cgcggcgctg gcagcggtgt ccctctcccg actggagctg
     3361 agccgacagc tccagcagcg ccgggaagtc ctggccgcgc tgttcgacgc ctacgacggc
     3421 aagcacgatg agttcttcag catcatcttt ccgctgtacg acgagaagga gaactacgac
     3481 accctgagcg agctcctgga gcgtccttcc gacgacgccc tcatggcgga tccgttcttc
     3541 aagcagcggg tcaccgtgat gatgaggggc ctcgccatcc gggaccccga catcgtggcc
     3601 attctcctgt acaagaacgc gacccgaggc cgatacgtct acgacatccg ctaccagaac
     3661 ttcgaggccg tgtccccgac ctacccgttc tttccccagc tcagcgccaa acccgcggga
     3721 agggtggtgt acggggcgcg ggagctgggc tccggccgtg acgcgcgatc ggtctatggc
     3781 atcgccggaa cgcttggcac ccgggcgctc cgcatggatg cgggcaacat catggtggct
     3841 tacgatgccg ccccgttcac ccggatcctc cagcgtaacc agggatccga ggcggaccgc
     3901 tacttgatcc tggccagcgg cggagaggcg gtcttcgact ccaggggcgc gcacccgggg
     3961 cagagctcgg tgtatcggga gcttctggat gtcggcctcg ggaaccgacc gcgcgtgatc
     4021 gacgggacgc cgtactacgt gcagaccgtt cgggacccgg gacggacctc ggtcggcgtg
     4081 ctcctggtgc cgcggtccgt ggtgcgcagc gccgcggtgg ggatctcggt gacgatcttc
     4141 gccatctgta ccgcggtggc cgctctctcc gcgggcctct atctgctggc gggactcctg
     4201 gtgtcccgcc gcgtggcgga ggtggagcgg gccatggatc gcatcggatc gcacaacctg
     4261 ggctatcgca tcccccttcg cggccgggac gatgagttcg agcacatctc gcagcggttc
     4321 aaccgcatgt gcgacgagct tcagcgcaac atcgatcggc tgtacgtcta ccagctcaac
     4381 cagaagagcg ccgagctgag cgcgctgcag gcgcgcatca atccccactt cctctacaac
     4441 accctggagg cgatccgggc gaccttcgag gaggaaggca gcggagaggc gggggagatg
     4501 atcgtgctcc ttgccgacat gtttcgaggc atcacgcgcg cggatcccat cgtgaccctc
     4561 cgcgatgagc tgaacacctg tcgtacctat ctcgatctca tgacgcggcg gttctccgac
     4621 gggcttcagg tggagttcgc ggtggaagag cagatcctgg agctgggcac cgtgcggaac
     4681 ctcctccagc cggttgtcga aaactgcctc ctgca
//
LOCUS       DASRZS010000096         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1976031, whole genome shotgun sequence.
ACCESSION   DASRZS010000096 DASRZS010000000
VERSION     DASRZS010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1976031"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VFH83_02340"
     CDS             complement(<1..1008)
                     /locus_tag="VFH83_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6485228.1"
                     /translation="MKTRMWQAASPALAAVLLVLLAGCSPQAGQTGQAGASGAATLAR
                     TWREFSKHDSIQMACFEQGWTGPEKDKDMITPEIERRTNLLLKYEPITVSTQEDINQK
                     LNLMVASGDVPEVYFGSADDYSMQVYDKMGEAGLVWDVAGLIKGYKNISTLLAPELTF
                     YRHKDGKANYFIPTQTGRGGDLLHNPPQGLFLRDDLLAKLSMAYPTTPEELATYLQRC
                     TDEIKTIAGKPIIGWTCDEGFGGIEYAFVFPHFPVFTGQYPDAGLTFDVRDNFRVVNY
                     LYTDSPELMRAAKYVSALYRKGLIDREILTQKRAQYEEKISSGRVVAFNGDSWVMNSL
                     SD"
     gene            complement(1074..1967)
                     /locus_tag="VFH83_02345"
     CDS             complement(1074..1967)
                     /locus_tag="VFH83_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485229.1"
                     /translation="MKPTAGQKVFGVFNAGSLLVFCVLCIYPLVLVTALSFNDGMDAM
                     RGGIYLWPRVWSLENYRTVLKENRIITSALISVYRTVAGAGLAVILNALFAYALTKKD
                     LPGRGFFLWFILVPMYFGGGIIPYFLVCKALGLINNILVYVLPAIFGPFYILLIRVGL
                     LDVPASLEESAKMDGAGYATIFFRIYFPLSGPILATVALLSGLGHWNDWFDGTVLVLT
                     SRLWPLQTLLLHIIQGADIMSFFKSRNIAMAGSFMKKVPITVESLKMAMLVLTVAPIV
                     ALYPFLQRYYIKGAMVGSLKG"
     gene            complement(1964..2893)
                     /locus_tag="VFH83_02350"
     CDS             complement(1964..2893)
                     /locus_tag="VFH83_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6485230.1"
                     /translation="MIERRGRTLARAWNYRVLYLFLLPGVLCFLAFSYAPMAGIVMVF
                     QRYDPVSGFLGSPWVGLANFARLFSAPTFMRALRNTLVISLLKLAIEFPLPILFALLL
                     NELRSTRFRRVVQTVTYLPSLISWVIVAGLWYKLLSPDNGVVNQLLMGIGITREPVFF
                     MQSTRWFYPVIIFTDVWKNLGFQSIFYLAAISVVDPELYEAAVMDGAGRVRQALSITL
                     PAMRTTIVLLVILDASSLVNAGFDQLWTMGNLAVREVGDILDTAVLRTLTQGSFQDLP
                     MGAAMGLFKSVVGLALFLLANGIARALRAESVL"
BASE COUNT          590 a          912 c         1009 g          517 t
ORIGIN      
        1 gtcggaaagc gagttcatga cccaggagtc cccattgaaa gccacgaccc ggcccgacga
       61 gatcttctcc tcgtactgtg cccgcttctg cgtgagaatc tcgcggtcga tcagcccctt
      121 gcggtagagg gcgctcacgt acttggcggc ccgcatgagc tctggggagt ccgtgtagag
      181 gtagttgacg acgcggaagt tgtcacggac gtcgaaggtg agcccggcat ccgggtactg
      241 gcccgtgaat acggggaagt gggggaagac gaaggcgtac tcgataccgc cgaacccctc
      301 gtcacaggtc cacccgatga tcggcttgcc ggcgatggtc ttgatctcgt ccgtgcaccg
      361 ctgcaggtac gtggccagct cctccggggt cgtcgggtag gccatggaga gcttcgcgag
      421 gaggtcgtcc cggaggaaga gaccctgggg cgggttgtgc agcaggtcac ccccccgtcc
      481 ggtctgggtg gggatgaagt agttcgcctt cccgtccttg tgccggtaga aggtgagctc
      541 gggcgccagg agggtcgaga tgttcttgta ccccttgatc aggcccgcga cgtcccagac
      601 gagaccggcc tctcccatct tgtcgtacac ctgcatgctg tagtcgtcgg cgctcccgaa
      661 gtagacctcg ggaacgtcgc cgctggccac catgaggttc agcttctggt tgatgtcctc
      721 ctgcgtggag acggtgatgg gctcgtactt cagcagcagg ttcgttctcc tctcgatctc
      781 gggagtgatc atgtccttgt ccttctcggg acctgtccat ccctgttcga agcatgccat
      841 ctggatggag tcatgcttcg agaactcccg ccacgtcctg gcgagagtcg cggcgccgga
      901 cgccccggcc tgcccggtct ggcccgcctg cggcgagcag ccagcgagaa gcacgagaag
      961 cacggccgcc agggccgggc ttgccgcctg ccacattctc gttttcatct ttcactcctc
     1021 ctccttgacg aacatcgcgg ccggcgtccc ggtggaactc ggcctggacc ggcctaacct
     1081 ttcagcgagc ccaccatggc ccccttgatg tagtagcgct gcaggaacgg gtagagcgcg
     1141 acgatgggcg ccacggtcag cacgagcatt gccatcttca gagactccac cgtgatcggg
     1201 acctttttca tgaacgaccc ggccatggcg atattcctgc tcttgaagaa gctcatgatg
     1261 tccgcgccct ggatgatgtg cagcaggagc gtctgcaggg gccagagccg tgacgtcagg
     1321 accaggacgg tcccgtcgaa ccagtcgttc cagtgcccca ggccggacag gagcgcgaca
     1381 gtggccagga tgggccccga gagagggaag tagatccgga agaagatcgt cgcgtagccg
     1441 gccccgtcca tcttggccga ttcctccagg gaggcgggca cgtcgaggag acccaccctg
     1501 atgaggagga tgtaaaaggg accgaagatc gcgggaagca cgtacacgag gatgttgttg
     1561 atgagcccga gcgccttgca gaccaggaaa taggggatga tgccgcctcc gaagtacatg
     1621 ggcacgagga tgaaccagag gaagaagcct ctccccggca ggtccttctt ggtgagcgcg
     1681 taggcaaaca gcgcgttcag gatgaccgcg aggcccgcgc cggccacggt acggtagacg
     1741 gagatgagcg cgctggtgat gatcctgttc tctttcagga ccgtgcgata gttctccagg
     1801 gaccacaccc gcggccacag gtagatccct cccctcattg cgtccatgcc gtcgttgaac
     1861 gagagcgcgg tcaccagcac gaggggatag atgcagagga cgcagaagac gagcagcgag
     1921 ccggcgttga acaccccgaa gaccttctgg cccgccgtcg gtttcacagt accgactccg
     1981 cgcgaagggc ccgtgcgatc ccgttggcca gaaggaagag cgccaggccc accacggact
     2041 tgaacagccc catcgcggct cccatcggga ggtcctggaa gctcccctgc gtgagtgtcc
     2101 tcagcaccgc cgtgtcgagg atgtcgccca cctcccgaac cgcgaggttc cccatggtcc
     2161 agagctggtc gaacccggcg ttgacgaggc tggaagcatc caggatgacc agcaggacga
     2221 tggtggtccg catcgcggga agggtgatgg agagggcctg gcgcaccctg ccggcgccgt
     2281 ccatgacggc ggcctcgtac agctcggggt ccacgacgga gatcgcggcg aggtagaaga
     2341 tggactggaa gccgaggttc ttccagacat cggtgaaaat gatcacgggg tagaaccagc
     2401 gggtgctctg catgaagaag acaggctcgc gcgtgatgcc gatccccatg aggagctggt
     2461 tcacgacgcc gttgtcgggg gaaagcagct tgtaccagag cccggccacg atcacccagg
     2521 agatgaggct cggcaggtag gtcaccgtct gcacgacgcg gcggaaccgc gtggaccgca
     2581 gctcgttgag caggagggca aagaggatgg ggagcgggaa ctcgatggcg agcttgagca
     2641 ggctgatgac cagggtgttg cgcagggcgc gcatgaaggt gggagcgctg aacaggcgcg
     2701 cgaagttggc caggcccacc cacgggctgc ccaggaagcc cgaaacgggg tcgtaccgct
     2761 ggaagaccat gacgatccct gccatgggtg cgtaggagaa agcgaggaaa cagagcaccc
     2821 cgggaaggag gaagaggtac aggacacggt agttccaggc cctcgcgagc gtccgtccgc
     2881 gtctttcgat catgggcgca acccgcttgc gcgatccgtt ctcccgcatg tcacgagacc
     2941 ggcacctccg ctgggtcatc atggttcgat tgcaggcggc accatccact ggatgcgggt
     3001 cagtataaac cctatttcag aaaatgca
//
LOCUS       DASRZS010000097         3625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2746058, whole genome shotgun sequence.
ACCESSION   DASRZS010000097 DASRZS010000000
VERSION     DASRZS010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2746058"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1901
                     /locus_tag="VFH83_02355"
     CDS             195..1901
                     /locus_tag="VFH83_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6485231.1"
                     /translation="MTTQDLWNRVRADLIGGDADIETPFGSRRLTYADHTASGRAVAG
                     IEAYLGRVLELYGNTHTEDDATGMVTSERLRQAESTIKRLVNAGPDHRIIAVGAGTTG
                     AVHRLQQILGVYLPPAGKDQFQRLLTERLGEAGCRELSAWLRSRRPVVFVGPYEHHSN
                     EVSWRECFAEVVEIDLDGHGRLDLEDLRRTLGRDEYRGRRLIGAFSAASNVSGVRTPV
                     HEVARILHEHGAVALFDYAAAAPYERLDMSRDPASTLDAVYFSPHKFVGGPGSCGILV
                     IHKRLYREDLPPTVGAGGTVDFVDFDGQTYASDIEVREKAGTPPILQTLRASLAMELA
                     EALGYDRIASTEASHVRLAHQLLGSHPAIELMGDVGLADRLPIFSFNIRVGDSWLHPR
                     YVTLLLNDLFGIQSRAGCSCAAPYGHRLLHIDSQKSQEIRRTILRGNVGLKPGWARVS
                     FHFLATEAEVRFICRAIRFVADQGSRFLARYRFDVRTGRWRHRDARQPETSFGLAEAL
                     GGGEVRRGGGRLRDDRELDALFESYLTEAERLAAALGAADLRHTEERLIPFVYSQEEG
                     AG"
     gene            2002..>3625
                     /locus_tag="VFH83_02360"
     CDS             2002..>3625
                     /locus_tag="VFH83_02360"
                     /inference="COORDINATES: protein motif:HMM:NF025681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP domain-containing protein"
                     /protein_id="HET6485232.1"
                     /translation="MTLSHAGVSGIFPLFDRPEVLTVLDVRPYSWEEQLTAACLQGLI
                     NRTKPRVYLVYDDVVDRQWLEIYKTDHGIPYREVATFQDLVKEYASELYGFAVCHEEM
                     PHTLNVAQTWGSLHNAVPATQRVAAVLERCGMQKVRDFRGMFKTRVDAYQWAVDNLLP
                     RCNKRILGHNCTEGWYLARTHKFIPHVKDYLVAVQAFTFDLSARKRDRAEYQLFEKIL
                     SSMPEDGALLGWHCAKCWESEYVALAARHGFFVFCNLRSPNYSAHAGVRTPGFRFTQK
                     HTSPTSIKKVEHKVYVTFIHSDGDAIWAENNFYAHNWLDSQRGRFPCTWEMQPYALDL
                     APGQLEYYYRTRSDKDYFIAGPSGAGYTAPIINSQRQRFLTNTQSYLKRCGLRSILIM
                     NRDPYQAFEELDNGAFPMEAARAIPEALGFIHGYGGNSFGQSEFAGEIPYVQTTQFVT
                     DSHDIYEEVSKFARLVSRRPLFVAVHVRESANFASLRSTCDRLDADIFKVVNVDEFLL
                     TIRKAREMGALGELSVASPELDRHYRKRTESWW"
BASE COUNT          625 a         1200 c         1180 g          620 t
ORIGIN      
        1 ctgtgcgtag ctggggatcg agctcattct catcctttcg gtttcatcat aatgtattga
       61 ggttatgaca atttatgggt ttgcgcgaga cctgtcaagt tggcacgatg ccaccctggg
      121 aagagcgtct ccagaaacaa agcacgcaaa ctccagtcgg gtcatggtac agtgtttcct
      181 cggacgatag caccatgaca acgcaagact tgtggaaccg ggtccgagcc gatctgatcg
      241 gcggtgacgc ggacatcgaa acgcccttcg ggtcgaggcg cctgacctac gcggaccaca
      301 cggcatccgg ccgggccgtt gccggcatcg aggcctacct gggccgggtg ctcgagctct
      361 acggcaacac ccatacggag gacgacgcta cgggcatggt cacctccgag cgtctgcggc
      421 aggcggagtc gacgatcaaa cgcctggtca acgcggggcc ggatcaccgc atcatcgccg
      481 tgggggccgg gaccacgggc gccgtgcacc ggctccagca gatcctaggc gtgtacctcc
      541 cgccggcggg caaggaccag ttccagcggc tcctgaccga gcgcctggga gaggccggct
      601 gccgcgagct ttccgcctgg ctgcggtccc gacgccccgt ggtgttcgtc ggcccctacg
      661 agcaccactc caacgaggtc tcctggcgcg agtgtttcgc cgaggtcgtg gagatcgacc
      721 tggacggcca cggtcggttg gacctggagg acctgcggcg cacgctgggc cgggacgagt
      781 atcgcggccg gcgtctcatc ggcgccttct ccgccgcgtc gaacgtctcc ggcgtgagga
      841 cgccggtgca cgaggtggcc cggatcctgc acgagcacgg cgccgtcgcg ttgttcgact
      901 atgcggcggc ggccccgtac gagcgcctgg atatgagccg ggaccctgcc tcgaccctgg
      961 atgcggtcta cttctcgccg cacaagttcg tgggcggccc gggaagctgc ggcatcctgg
     1021 tgatccacaa gcgcctgtac cgcgaggacc tccctcccac cgtcggagcc ggcggcacgg
     1081 tggacttcgt ggacttcgac gggcagacct acgcttcgga catcgaggtc cgggagaagg
     1141 ccggcacgcc tcccatcctg cagacccttc gggcatccct ggcgatggag ctggcggagg
     1201 ctttggggta cgatcggatt gcgagcaccg aggcatccca cgtccgcctg gcccaccagc
     1261 tcctgggatc ccacccggcc atcgagctca tgggggacgt gggcctggcg gatcgcctcc
     1321 ccatcttctc cttcaacatt cgcgtcgggg attcgtggct gcacccgcga tacgtgaccc
     1381 tgctcctcaa cgacctgttc ggaatccaga gccgggcggg ctgctcctgc gccgccccgt
     1441 atgggcaccg cctcctgcac atcgattcac agaagtccca ggagatccga cgcacgatcc
     1501 ttcgcgggaa cgttggcctc aagccgggat gggcgcgggt gagcttccac ttcctcgcca
     1561 cggaggcgga agtccggttc atctgccgcg ccatccggtt cgtggcggac cagggatcgc
     1621 gattcctggc gcggtaccgg ttcgacgttc gtaccgggcg gtggcggcat cgggacgccc
     1681 gccagccgga aacgagcttc ggtctggcgg aggctctggg cggaggggag gtccgccgcg
     1741 gaggcggccg tctccgggac gaccgggagc tggatgcgct attcgagagt tatctcacgg
     1801 aagcggagag gctggcggca gcgctgggcg ccgcggatct ccgccatacc gaagagcggc
     1861 tgatcccctt cgtctacagt caggaagagg gcgccgggta gacgctcccg gccggcaagc
     1921 gactcagaag ttactcctgt ttttggatgc gggcgtccgg ggagtgctat gctttctatg
     1981 aatgccctgg gagggcccgc catgacgctt tcacacgccg gagtctccgg catattcccc
     2041 ctcttcgatc gccctgaggt gttgaccgtg ctggacgttc gtccctactc gtgggaggag
     2101 cagctcaccg cggcctgtct gcagggactg atcaaccgga ccaagccgag ggtctacctc
     2161 gtctacgatg acgtggtgga ccggcaatgg cttgagatct acaagaccga ccacgggatc
     2221 ccctatcggg aggtcgccac gttccaggac ctggtcaagg agtacgcctc ggagctctac
     2281 ggctttgccg tctgccacga ggaaatgccg cacaccctga acgtggccca gacgtggggg
     2341 tctctgcaca acgcggtccc ggcgacacag cgggtcgccg ccgtcctgga gcgctgcggg
     2401 atgcagaagg tgcgggactt ccgcgggatg ttcaagaccc gggtggacgc ataccagtgg
     2461 gccgtggaca acctgctgcc gcgctgcaac aaacggatcc tcgggcacaa ctgcacggag
     2521 gggtggtacc tcgccaggac tcacaagttc atcccccatg tcaaggatta cctggtggcc
     2581 gtgcaggcgt tcacgttcga cctgtccgcg cgcaaacgcg accgggcgga gtaccagctc
     2641 ttcgagaaga tcctctcctc catgccggag gacggagcgc tcctgggctg gcactgcgcc
     2701 aagtgttggg agagcgagta cgtggccctg gccgcgcgcc acgggttctt cgtcttctgc
     2761 aacctccgat ctcccaacta cagcgcgcac gccggcgtgc ggacgccggg gttccgcttc
     2821 acgcagaagc acaccagccc gaccagcatc aagaaggtgg agcacaaggt ctacgtcacc
     2881 ttcatccatt cggacgggga cgccatctgg gcggagaaca acttctacgc ccacaactgg
     2941 ctggacagcc agcggggtcg gtttccgtgc acctgggaga tgcagccgta cgccctggac
     3001 ctggcgcccg ggcagctcga atactactat cggacccgct cggacaagga ctacttcatt
     3061 gccggtccgt ccggcgcggg atacaccgcc cccatcatca acagccagag gcagcgattc
     3121 ctgaccaaca cgcagtccta cctgaagcgc tgcgggcttc ggtccatcct catcatgaac
     3181 cgcgacccct accaggcctt cgaggagctg gacaacggcg cattccccat ggaggcggcc
     3241 cgagccatcc cggaggcgct cgggttcatc catggctatg gcgggaactc cttcggtcag
     3301 agcgagttcg cgggagagat cccgtacgtg cagaccaccc agttcgtcac cgacagccac
     3361 gacatctacg aagaggtatc caagttcgcg cggttggtct cccgtcggcc gctgttcgtg
     3421 gcggtccacg tgcgggagag cgcgaacttc gcctccctgc gctccacctg tgaccgcctg
     3481 gacgccgaca tcttcaaggt ggtgaacgtc gacgagttcc tcctgaccat ccgaaaggcc
     3541 cgcgagatgg gcgcgctcgg cgagctctcc gtggcaagcc ccgagctgga ccgccactac
     3601 cggaaacgga ccgaaagctg gtggg
//
LOCUS       DASRZS010000098         3624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3133961, whole genome shotgun sequence.
ACCESSION   DASRZS010000098 DASRZS010000000
VERSION     DASRZS010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3133961"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VFH83_02365"
     CDS             <1..1221
                     /locus_tag="VFH83_02365"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HET6485233.1"
                     /translation="SYAVLHLDFDGFKTINDRFGHSAGDAFLCEIASRLKHSVREVDL
                     AARVGGDEFVVLLDGFVTRENLEEIVDRIVDNLSVPITFGSHAVVPAASIGIVIGRPD
                     YKDPQSILHDTEIALHRAKSTGRNLRVVFSEEMRLQVDERNLLSAALRTAIASGGISL
                     AFQPIVRLDGAPTGWEVLARWRHETLGPIGPDRFIPIAEETGLILPLGAYILLETLKV
                     AAALREQGLLDGARAGAGFFAVNVSAIQFGQRDFAELVLSSLDRLGLPRAALHLELTE
                     SAIMENRDIATRVIEQLSAEGISFKLDDFGTGYSSLGYLHRIPISCVKIDRSFISRMD
                     ASKGDENSAGMVRGMIALSHELRKTVVAEGIETEPQAAMLREYGCDFGQGWHFGKPMD
                     RGALVESLRAARVR"
     gene            complement(1286..2083)
                     /locus_tag="VFH83_02370"
     CDS             complement(1286..2083)
                     /locus_tag="VFH83_02370"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6485234.1"
                     /translation="MQATFKKLSADEVAGYRTEGYLLYHRQVFDRPKLHRLRTLFEEK
                     IANLKPDERPEQMDVPHFVDARLFEWIFAPEVLDIVEDVIGPDIDLFSTHLIAKPAGG
                     SMRVPWHEDSAYWRDWIDPMEVVTVWLALDGSDEENGCMYVVPRSHAKGFSEYSNVDP
                     DKNLFMTEIRKGQFDESKAVPLVLAEGEASLHHAKLIHGSPANTSKRRRMGYTMRYIA
                     STTRLNPDNPAKQQLYHARGRDHGINRYADPSKSYPELLVGRMTKGH"
     gene            2219..3244
                     /locus_tag="VFH83_02375"
     CDS             2219..3244
                     /locus_tag="VFH83_02375"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET6485235.1"
                     /translation="MTQIEWYRIPVDKGTLKSFTEKSDLKGFLQAGSFLLVFLVTTSV
                     SFYFFWRHMWVAMVISCYLHSVFHQMVGMSAAVHELNHGTVFRTKRINEFFYKLFCFL
                     TWNNPVHFRASHALHHQYTVFRDIDKEVIQEPVKLTMNWRNYISWFTFDYTWFWTLVR
                     GNVLHFLGKGNVDFFSWNPLFAEDDPRRKQMIRWARIVLPTYVALIVVFSVLHLWVLI
                     YLIIFGNFFATFMGRLTFLIQHMGLDENVPDWRMVCHTVKVHPFIGWLYWHMNFHIEH
                     HMYAAVPCYNLPKFHEAIKDQLPEAPNGFINGLKLVGSIRKRQEEDPDYIYVPRFPPT
                     ATPPKTK"
     gene            complement(3294..>3624)
                     /locus_tag="VFH83_02380"
     CDS             complement(3294..>3624)
                     /locus_tag="VFH83_02380"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HET6485236.1"
                     /translation="TFPAGSGPLGIVASPDGRFLYVTNSGGGSGCSYAIGAGGTLTPL
                     VSGFFVTGVFPAGIAITPDGRYLYVASVSGNWVAGFSIGADGHVSPLSTPTFPAGTAP
                     FGIVVVP"
BASE COUNT          620 a         1218 c         1150 g          636 t
ORIGIN      
        1 tcctacgcgg ttctccacct ggacttcgac gggttcaaga cgatcaacga caggttcggt
       61 cacagcgcgg gagacgcgtt cctgtgcgag atcgcctcgc ggctgaagca cagcgtgcgg
      121 gaggtcgacc tcgccgcccg cgtgggggga gacgagttcg tcgtcctgct ggacgggttc
      181 gtcacccggg agaacctgga agagatcgtg gaccgcatcg tcgacaacct gagcgtcccc
      241 atcaccttcg gctcccacgc ggtggtcccc gccgcgagca tcggcatcgt catcggccgg
      301 ccggattaca aggatccgca gtccatcctg cacgacacgg agatcgcgct gcacagggcg
      361 aagtccaccg ggaggaacct ccgggtggtc ttctccgagg agatgcggct ccaggtggac
      421 gagcgcaacc tcctgtcggc cgcgctgcgg accgccatcg cctccggcgg gatctcgctg
      481 gcgttccagc ccatcgtccg cctggacggc gcccccaccg gctgggaggt cctcgcgcgc
      541 tggcgccacg agacgctggg ccccatcggt ccggaccggt tcatccccat cgcggaggag
      601 accggcctga tcctccccct gggtgcgtac atcctcctgg agacgctgaa ggtcgccgcc
      661 gccctgcggg agcagggcct cctcgacggg gcgcgagcgg gggcgggatt cttcgccgtc
      721 aacgtctcgg ccatccagtt cggccagcgc gatttcgcgg agctggtgct ctcctccctg
      781 gaccggctgg ggctcccgcg cgccgcgctg cacctggagc tcacggagag cgccatcatg
      841 gagaaccgcg acatcgcgac gcgcgtcatc gagcagctct ccgcggaggg catcagcttc
      901 aagctcgacg acttcggcac cggctactcg tcactcgggt acctgcaccg gatccccatc
      961 agctgcgtga agatcgaccg cagcttcatc tcgcggatgg atgcgtcgaa gggcgacgag
     1021 aactcggccg gcatggtgcg cggcatgatc gccctctcac acgagctgcg caagaccgtg
     1081 gtggcggaag gcatcgagac cgagccccag gcggcgatgc tgcgggagta cggctgcgat
     1141 ttcggccagg gctggcattt cggcaagccc atggaccgcg gcgccctggt ggaatcgctg
     1201 cgcgccgccc gggtgcgata ggcggcggcc accgcgcgtg gggcggaggg cgcaggcagc
     1261 ccatccccgc gcggctcccg ctctctcagt gccccttggt catgcggccg acgagcagct
     1321 cgggatagct cttcgagggg tcggcatacc ggttgatgcc atggtccctg ccccgtgcgt
     1381 ggtagagctg ctgcttggcg ggattgtcgg gattcagccg cgtggtagac gcgatatacc
     1441 tcatcgtgta tcccatgcgg cggcgcttgc tggtgttggc cgggctgcca tggatcagct
     1501 tggcgtggtg gaggctcgcc tccccctccg ccaggaccag cggcacggcc ttcgactcgt
     1561 cgaactgccc cttgcggatc tccgtcatga agaggttctt gtccgggtcc acgttggagt
     1621 attccgagaa acccttggcg tggctgcggg ggaccacgta catgcagccg ttttcctcgt
     1681 ccgagccgtc cagcgccagc cagaccgtca ccacctccat gggatcgatc cagtcacgcc
     1741 agtaggcgga gtcctcgtgc cagggcaccc gcatgctgcc gccggccggc ttggcgatga
     1801 ggtgcgtgga gaacaggtcg atatcggggc cgatgacatc ctcgacgatg tccagcacct
     1861 cgggggcgaa gatccattcg aacaggcgcg cgtccacgaa atggggcacg tccatctgtt
     1921 cgggacgttc gtccggcttc aggttggcga tcttctcctc gaagagggta cgcagccggt
     1981 ggagcttcgg ccggtcgaag acctgtcgat ggtacagcag gtacccttcg gtccggtagc
     2041 ccgccacctc gtcggcgctg agcttcttga acgttgcctg catctgagcc tccgaagctg
     2101 aatgtaatag tccggcgcgg cagccgcata gcgtcccgcg gtgcgcgccg tcttttcttg
     2161 actcgggcct ctcacgtttc taccttcgcg tgagggagag gaaacacgcg caatggccgt
     2221 gacccagata gagtggtacc ggatccccgt ggacaagggg accctcaagt ctttcacgga
     2281 gaagagcgac ctgaagggat tcctccaggc cggaagcttc ctcctggtct ttctcgtcac
     2341 cacctccgtc tccttctatt tcttctggag gcacatgtgg gtggcgatgg tgatctcctg
     2401 ctacctgcac tccgtgttcc accagatggt gggaatgtcc gccgccgtgc acgagctcaa
     2461 ccacggcacc gtcttcagga ccaagcggat caacgagttc ttctacaagc tgttctgctt
     2521 cctcacctgg aacaacccgg tccacttccg tgccagccac gcgctgcacc accagtacac
     2581 cgtgttccgg gacatcgaca aggaagtcat ccaggagccg gtgaagctca ccatgaactg
     2641 gcgcaactac atcagctggt tcacgttcga ctacacgtgg ttctggaccc tcgtccgcgg
     2701 caacgtgctg cacttcctgg gcaaggggaa cgtggacttc ttctcgtgga accccctgtt
     2761 cgccgaagac gacccgcgac ggaaacaaat gatccgctgg gcccgcatcg tgctgcccac
     2821 ctacgtggcc ctgatcgtcg tgttcagcgt cctgcacctc tgggtcctca tctacctcat
     2881 catcttcggg aacttcttcg ccaccttcat gggcaggctg acatttctca tccagcacat
     2941 gggcctggac gagaacgtgc cggactggcg gatggtctgc cacacggtga aggtgcaccc
     3001 cttcatcggc tggctgtact ggcacatgaa ctttcacatc gagcaccaca tgtacgccgc
     3061 cgtgccctgc tacaacctcc cgaagttcca cgaggccatc aaggaccagc tccccgaggc
     3121 cccgaacgga ttcatcaatg gactgaagct cgtgggatcg atcaggaaac ggcaggagga
     3181 ggaccccgac tacatctacg tcccccggtt cccgccgacg gcaaccccgc cgaagacgaa
     3241 atgagggatg gcgccgccgc cggggcgccg cgggccgacg gcggcgcccc ggcctatgga
     3301 accaccacga ttccgaacgg ggccgtcccg gcgggaaagg tcggcgtgct caggggactg
     3361 acgtggccgt cagccccgat ggagaagccg gccacccaat tgcccgagac gctggcgacg
     3421 tagaggtacc tgccatccgg cgtgatggcg atcccggcag gaaaaacccc ggtgacgaaa
     3481 aagccactca ccaggggcgt gagggtgcct ccggcgccga ttgcgtacga gcacccggag
     3541 ccgcccccgg aattcgtcac gtacaggaac cggccgtcgg gcgacgccac gatgccgaga
     3601 ggccccgatc ctgcaggaaa cgtc
//
LOCUS       DASRZS010000099         3219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2747201, whole genome shotgun sequence.
ACCESSION   DASRZS010000099 DASRZS010000000
VERSION     DASRZS010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2747201"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="VFH83_02385"
     CDS             <1..1437
                     /locus_tag="VFH83_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HET6485237.1"
                     /translation="CGEGCCEGSCTHVWNYAYALPFLFPRLERSMRSLDYRFNEGDGG
                     GMSFRLQLPLGRARSTFRPCADGQFGGVVKAYRDWKISGDTGWLRGIWPALRRSIEFA
                     WSPSNRDRWDADQDGVLEGRQHHTLDMELFGPNSWLTGFYLAALKAGALMAEHLGERD
                     FAHRLESLFQRGKRWTDANLFNGSYYHQRVDLRDRSLLEKYTEGLTLIGEGTVGAYWN
                     HEADEMKYQIAEGCEIDQVLAQWHANLCGLGEIFDPEQTRTALRSIHRNNFKRGMREF
                     ANPCRLYCLNDEAGLVVCDWPDGTYKPVVPLPYAEETQNGYEYAAAIQMIQSGLVDEG
                     LECVAAIRSRYDGEKRNPWNEFECGSNYARSMASFALLPALSGFEYDLVEGMIGFSPR
                     IRRDDFSCLWSLDPGWGVFTHRGRTLTVRLLGGSLTVVRFKSDLLQSPARAAQANGGD
                     LPFDHRPGEVRFASPVRVEAGQALEITT"
     gene            1487..>3219
                     /locus_tag="VFH83_02390"
     CDS             1487..>3219
                     /locus_tag="VFH83_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET6485238.1"
                     /translation="MEETMTVPRQEHPRPQFVRSDWLSLNGAWQFEIDPGNSGLERSL
                     LSRALSGTIQVPFCPESKLSGIGNTDFLNAVWYRRAVTVPAAWKGKRVLLHFEAADYD
                     TTVWVGQTQVGRHRGGFTPFTFEITNEAAPGKEVTLAVRCRDDHRLPKPRGKQSELHD
                     RYSCLYDRTTGIWQTVWLEAVGASYLETPRITPDLGRSLIRVEQPVVRASGARLSVRA
                     VLLDDQGEVARAEVAVGADFTPALDLGVPAGRRRLWQPSDPHLYRLLLSLTDASGAVV
                     DQLESYAGLRSVTIDGNRILLNGRPFFQRLILDQGFYPDSLYTAPDDAALKRDIQLSL
                     DAGFNGARLHQKVFEQRFLYHADRMGYLVWGELPDWGVDRERADASTVAQWLEALHRD
                     YSHPCIVGWCPLNETGQPLKEGIEGLDDLTLALYEATKGSDRTRPVLDASGYSHRVAL
                     PDVFDCHDYEQDVAKFTSNHARTFQGAPFYNGPADRPWSLPYRGQPYLVSEFGGIWWN
                     PKAAENEPSWGYGTRPRTVEEFYARFEGLCAALLANPGVCGYCYTQLTDVFQEQNGVY
                     YFDRTPKFDLA"
BASE COUNT          554 a         1066 c         1057 g          542 t
ORIGIN      
        1 tgcggcgaag gctgctgtga gggctcctgt acgcacgtct ggaactacgc ctatgccctt
       61 ccctttctct ttccccggct cgagcgatcc atgcgttccc tggactaccg gttcaacgaa
      121 ggggacggcg ggggcatgag ctttcgcctc cagctaccgc tgggcagggc ccgctcgacg
      181 ttccggccat gcgcggatgg gcagttcgga ggggtggtca aagcctatcg ggactggaag
      241 atcagcgggg acaccggctg gctgcggggg atctggccgg cactccgccg ttcgatcgag
      301 ttcgcctggt cgccctccaa ccgggaccgc tgggatgccg accaggacgg ggtcctggag
      361 ggtagacagc accacaccct ggacatggag ctgttcgggc ccaactcctg gctcacgggg
      421 ttctaccttg ccgccctcaa ggcgggcgcc ctcatggcgg agcacctggg agagagggac
      481 ttcgcccatc ggctcgagag tctcttccag cgcggcaaac ggtggaccga cgcgaatctc
      541 ttcaacggaa gctactacca tcagcgcgtg gacctgcggg accggtccct gctggagaag
      601 tacacggaag gcctgaccct gatcggagag ggcaccgtgg gcgcgtactg gaaccacgag
      661 gccgacgaga tgaaatacca gatcgccgag ggctgcgaga tcgatcaggt cctggcccag
      721 tggcacgcca acctctgcgg tctgggagag atcttcgatc ccgagcagac gcggaccgcc
      781 ctgcgctcga tccaccgcaa caacttcaaa cgcgggatgc gggagttcgc gaacccctgc
      841 cgcctgtact gcctcaacga cgaggccggg ctcgtggtat gcgactggcc cgatggcacc
      901 tacaagcccg tcgtcccgct tccgtacgcc gaggagacgc agaacggata cgagtacgcg
      961 gcggccatcc agatgatcca gtcggggctg gtcgatgagg gcctggaatg cgtggcggcc
     1021 atccggtccc gatacgatgg agagaaacgc aacccctgga acgagttcga gtgcggcagc
     1081 aactacgccc ggtccatggc gagcttcgcc cttctgcccg cgttgagcgg gttcgagtac
     1141 gacctggtgg aagggatgat cggtttcagc ccccggatcc gaagggacga cttctcctgc
     1201 ctctggtccc tggatcccgg gtggggagtg ttcacccacc ggggccgcac gctgaccgtc
     1261 cgtctgctgg gcgggtcgtt gacggtggtc cgtttcaaga gcgatctcct gcaaagcccc
     1321 gcccgcgctg cccaggcaaa cggcggagac ctgccgttcg accatcggcc cggagaagtc
     1381 cgattcgcgt caccggttcg cgtggaagcg gggcaggcct tggagatcac cacctgagcg
     1441 ccgccccctg gaatccgcgc ctggagaggt cccaggagat attgcaatgg aggagacgat
     1501 gaccgtccca cgacaggagc atcccagacc gcagttcgtc cggtccgact ggctgagcct
     1561 gaacggcgcc tggcaattcg agatcgaccc tggaaacagc gggctcgagc gcagcctgct
     1621 ctcccgcgct ctatcgggca ccatccaggt ccccttctgc ccggagtcga agctgtcggg
     1681 gatcgggaac accgacttcc tgaacgcggt gtggtatcgg cgagccgtga ccgtgcccgc
     1741 ggcctggaaa ggcaaacggg tgctccttca cttcgaagcc gcggactacg ataccacggt
     1801 ctgggtcggc cagacgcagg taggacgcca tcgcggcgga ttcacgccgt tcaccttcga
     1861 gatcacgaac gaggcggcgc cgggcaagga ggtcacgctg gccgtgcggt gccgcgacga
     1921 ccatcgactg cccaagccgc gcggcaagca gtccgagctc cacgaccggt actcctgtct
     1981 ctacgatcgc accaccggca tctggcagac cgtatggctg gaggcggtgg gcgcctctta
     2041 tctggagact ccgcggatca ctccagacct gggacgctcg ctgattcgag tcgagcagcc
     2101 ggtggtcagg gcctctggag cgcggctttc cgttcgtgcc gttcttctcg acgatcaggg
     2161 agaggtggcg cgcgccgagg ttgccgtggg agcagacttc acccccgccc tggacctcgg
     2221 cgtgccggcg ggccgaaggc ggctctggca gccatccgac cctcacctct accggctgct
     2281 cttgagcctg acggacgcct ctggcgcggt ggtggaccag ctggaaagct acgccggcct
     2341 tcggtcggtc accatcgacg gcaaccgcat cctcctgaac ggcaggccct tcttccagag
     2401 gctgatcctg gatcagggct tctacccgga ctccctgtac accgcgccgg atgacgccgc
     2461 gctgaaacgg gacatccagc tctccctgga cgccgggttc aacggggccc gcctgcacca
     2521 aaaggtcttc gagcagcgct tcctctacca tgccgaccgg atgggttacc tggtctgggg
     2581 cgagcttccc gactggggcg tggaccgcga acgggctgat gcgagcaccg ttgcccagtg
     2641 gctcgaggcg ctccatcgcg actactccca tccgtgcatc gtgggatggt gcccgctcaa
     2701 cgagacgggc cagcccctca aggaggggat cgaagggttg gacgatctca ccctcgcgtt
     2761 gtacgaggcc accaaggggt ccgatcggac acgacccgtg ctggatgcct cgggctactc
     2821 ccaccgcgtg gcgctgccgg acgtcttcga ttgccacgac tacgagcagg acgtcgcgaa
     2881 gttcacgtcc aaccacgccc ggaccttcca gggcgcgccg ttctacaacg ggccggcgga
     2941 ccggccgtgg tctcttccct atcgcggcca gccctacctg gtgagtgagt tcggcgggat
     3001 ctggtggaat cccaaggcag cggaaaacga gccttcctgg ggctatggga cccgccccag
     3061 gaccgtcgag gagttctacg cgcgcttcga gggtttgtgt gccgcgctgc tggccaaccc
     3121 cggagtctgc gggtattgct atacccagct caccgacgtg ttccaggagc aaaacggcgt
     3181 gtactacttc gaccgcacgc cgaagttcga cctggccag
//
LOCUS       DASRZS010000100         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_440905, whole genome shotgun sequence.
ACCESSION   DASRZS010000100 DASRZS010000000
VERSION     DASRZS010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440905"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFH83_02395"
     CDS             <1..249
                     /locus_tag="VFH83_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485239.1"
                     /translation="SLSDSVEEPIPFDLADFTDSRYNLRLGRILAHAVRHPSLFPGLA
                     RLQRNTARAAEVAAAVVFALLGQEGGLVEDVRDEVGRT"
     gene            246..845
                     /locus_tag="VFH83_02400"
     CDS             246..845
                     /locus_tag="VFH83_02400"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6485240.1"
                     /translation="MKLVLIRHGQTEWNAQRRTQGQRDSPLTETGLRQADHLGQRLRT
                     LSPRAFFCSDTGRARKTADRITLANPGFPPVRPEPRLRELDFGEWEGLAHDEIRTRYP
                     DLYRIYRSDPARFRAPGGEAFPDVQMRFLSFAEELHPESDGTVIAVTHSGTIRVALLA
                     LTGRPLSEVWALPSVAETSVSVLSWERGAWRIESAAAVW"
     gene            885..3155
                     /locus_tag="VFH83_02405"
     CDS             885..3155
                     /locus_tag="VFH83_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET6485241.1"
                     /translation="MRTLSLNGYWDWHLPHGPTITRRVPSCYTCVGQAVFERDVELDL
                     AEHERAFLCFEGIHYTGTVSLNGAPLGEMLPYVPYRFEVTDRLAGRKNHLVVTVNDIT
                     AEYGPTGGWEDYGGISRDVSIEVHDRILVEDLQWLTTLTPGSEGHPGAHCTLTAWCSS
                     SGVRPSGATLRVTLSRSGAVAHQESRTLDLVGPATATFSFDLPHPDLWSPDAPNLYEL
                     RVSLAAGRLQDERRLSVGIRELRVDGSRFLLNGKEIFLKGVARHEMWGDAGFSLTEEQ
                     IEEDLGLIKRMGANFVRLVHYPHSRKTVEAADRIGLHLSEEPGLWWSDMADPVITGRA
                     LEIVRRTVLRDRNSPSVAAWLFFNECVLKDARDYLVNGRDLCRGLDPSRLISGANCMD
                     SQEARTVFDECGFDFYTQHPYSYEPEALVRAMEVLRGKPLVFTEWGGWYIHNNPNLLR
                     WFKRTIARAAHPAPGQPSLAGLCWWQWQDIFELHRGLPGCMDGVLSDGLVDRYRTRKP
                     MYATMAEIFDLIDRPYDPGWTVEHVGAGCAAVEHASDGHRVRDARSALIDLSPIVSSP
                     SQQAAWEYALSSVRKLEKSPQRKGVRNTGALLPVDVVSPAGLPVVVKAGRPIILAGDC
                     REVEVPIGRRTALLHLLGHTTYYDGFPVRGQGGAVVARYVLLFEDGTTTEVPLRNGME
                     LASASMLARHSRMNPIATEAPRVLRITIDPDWEIYQVNCYRLPLDGRKVLARLRFESL
                     SRDFHPLLYAVVAEDA"
BASE COUNT          510 a         1076 c         1077 g          516 t
ORIGIN      
        1 tccctcagcg actccgtgga ggagccgatc cccttcgacc tggcggattt cacggactcc
       61 cggtacaacc ttcggcttgg ccggatcctg gcccatgcgg tccgccatcc ctcgctcttc
      121 cccgggctgg caaggctcca acggaacacg gctcgagcag cggaggtggc cgccgccgtc
      181 gtcttcgccc tcttgggcca ggaaggcggg ctcgtggagg acgtccggga cgaggtcgga
      241 cgaacatgaa gctggtcttg atccggcacg ggcagaccga gtggaacgcg caacggcgga
      301 cccagggtca gcgggactcc ccgttgacgg agacggggct tcgccaggcc gaccacttgg
      361 ggcagaggct ccgaacgctg agcccgcgcg ccttcttctg cagcgacacg ggccgcgcgc
      421 gcaagaccgc ggaccgaatc acgctcgcca accccggctt tcccccggtg cggccggagc
      481 ctcgtctccg agagctcgac ttcggtgaat gggaaggttt ggcgcacgac gagatcagga
      541 cccggtatcc ggatctctac cggatctatc gcagcgatcc cgcccgtttt cgcgcgccgg
      601 gcggagaggc attcccggat gtgcagatgc gcttcctctc atttgccgag gagctccacc
      661 ccgagtcgga tgggaccgtg atcgcagtga cccacagcgg gacaatccgc gtcgcgctcc
      721 tggccctgac ggggagaccc ctctccgagg tctgggcgct gccgtcggtc gcggagacca
      781 gcgtctcagt cctgagctgg gagcgcggcg cctggcggat cgagtcggct gccgccgtgt
      841 ggtagcatcc gcgaccccgc ccgatgcgcg ggaaggagaa cggcatgagg acactctcgc
      901 tcaacggcta ctgggactgg catctccccc acggacccac gatcactcga agggtaccct
      961 cctgctacac ctgcgtgggg caggcggtgt ttgagcggga cgtcgagctg gacctggccg
     1021 agcacgagcg cgcgtttctc tgcttcgagg gcatccacta cacgggcacg gtgtcgctca
     1081 acggcgcccc gctcggggag atgctcccgt acgtccccta tcggttcgag gtcacggacc
     1141 gtctggcagg tcggaagaac cacctggtgg tgacggtgaa cgacatcacc gcggagtacg
     1201 ggcccacggg cgggtgggaa gattacggag ggatcagtcg ggacgtctcc attgaggtcc
     1261 acgatcggat cctggtcgag gacctccagt ggctcacgac gctcacgccg ggctcggaag
     1321 gccaccctgg ggcgcactgc acgctcaccg cgtggtgctc gtcatcgggg gtccggccct
     1381 ccggcgccac cctgcgggtg accctgtcga gaagcggcgc cgttgcgcac caggagtccc
     1441 gcacgctgga cctcgtggga cccgccacgg cgaccttctc cttcgacctg ccgcaccccg
     1501 atctctggtc gccggatgct ccgaacctct acgagcttcg cgtttccctg gccgccggcc
     1561 gcctccagga cgagcgtcgt ctctccgtgg ggatccggga gctccgggtg gacgggtccc
     1621 gattcctgct gaacggcaag gagatcttcc tcaagggagt ggcccgccac gagatgtggg
     1681 gagacgccgg gttctccctc acggaggagc agatcgagga ggacctgggt ctgatcaaac
     1741 ggatgggagc caacttcgtg cggctggtcc actacccgca cagccgcaag accgtggagg
     1801 cggcggaccg catcgggctc cacctgagcg aggagcccgg gctctggtgg tccgacatgg
     1861 cggatcccgt gatcacgggt cgggcgctgg agatcgtgcg caggacggtg ctgcgggacc
     1921 gcaacagccc cagcgtggcg gcgtggctgt tcttcaacga gtgcgtgctc aaggacgccc
     1981 gagactacct ggtgaacggt cgcgacctgt gccggggcct ggacccgtcg cgcctgatct
     2041 cgggagccaa ctgcatggac tcgcaggaag ctcgaaccgt tttcgacgag tgcggcttcg
     2101 acttctacac ccagcacccc tactcctatg agccggaggc gctggtccgc gccatggagg
     2161 tgctgagggg caagcccctg gtgttcacgg aatggggcgg ctggtacatc cacaacaacc
     2221 ccaacctgct gcggtggttc aagcggacca tcgcgcgcgc cgcgcacccg gcgccgggcc
     2281 agccgagcct ggccggcctg tgctggtggc agtggcagga catcttcgag ctgcaccgcg
     2341 gcctgccggg ctgcatggac ggtgtcctgt ccgatggtct ggtggaccgc taccggaccc
     2401 gaaagcccat gtacgccacc atggccgaga tcttcgacct gatcgaccgc ccgtacgatc
     2461 ccggctggac cgtcgagcac gtcggcgccg gatgcgctgc cgtcgaacat gcgtcggacg
     2521 gacatcgggt gcgggacgcg cgaagcgcgc tgatcgatct ctctccgatc gtatccagcc
     2581 cgtcgcagca ggcggcctgg gagtacgccc tctcatcggt ccgcaagctc gaaaagagcc
     2641 cccagcgcaa gggagtccgc aacacgggag cgctcctgcc cgtggatgtg gtatcgccgg
     2701 caggactgcc cgtggtagtg aaggcgggtc gccccatcat cctcgcggga gactgcagag
     2761 aggtcgaggt cccgatcggc cgaaggacgg ccctcctgca ccttctcggt cataccacgt
     2821 actacgacgg ctttccggtc cgtggccagg gcggcgccgt cgtggcacgc tacgtgctcc
     2881 tcttcgagga tggcacgacc acggaggtgc cccttcgcaa cggcatggag ctggcctccg
     2941 cgagcatgct ggcccgacac tcccggatga atcccatcgc cacggaggcg ccgagggtcc
     3001 tacgaatcac catcgatccg gactgggaga tttaccaggt caactgctac cgcctgccgc
     3061 tggacggccg aaaggtgctg gcgcgcctgc gtttcgagag cctgagccgg gacttccacc
     3121 ccctgttgta cgcggtggtc gctgaagatg cctgaggttc gcgtcgatcg gtccagcag
//
LOCUS       DASRZS010000101        11021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5445193, whole genome shotgun sequence.
ACCESSION   DASRZS010000101 DASRZS010000000
VERSION     DASRZS010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11021
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5445193"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VFH83_02410"
     CDS             <1..988
                     /locus_tag="VFH83_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6485242.1"
                     /translation="PLYEGKILDLNKYLTKQDLADFKPAAIAASTYKGVLCYLPILIN
                     SVSIVFNMDVVQKAGITADWVNTKFLSWDDFLSYCAKVKATGAWPYTMGPADAGIIDE
                     LSNWMYQAGGDWYNADYTKSATNTPQAIRMFKYLVSLWDNGYINPADKDKTETEIEQG
                     VFPAGGIGAVMVQNQNIPNAILKTNPAMHLVYAYPFKDKATSSNGTIAGYGSFNTTKN
                     PEAAAAWIKAITGPEGMTKIDKVVNFIPTRISLDASFKDMMKDPIFNRAVENTEWFVP
                     TVPPSPVGPAAIEEVKSGFQKMILHQASVEDAAADMTARINKLLDDYYKKTK"
     gene            1074..1976
                     /locus_tag="VFH83_02415"
     CDS             1074..1976
                     /locus_tag="VFH83_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485243.1"
                     /translation="MSRKKGLLYQVVTSWPSYVLVLPALAILFVFDIYPIIMTLIMST
                     RFYKNVFDSVYVGLGNYQYVLTDSSFWNAMKVTGIYTLFLVPGLLVFSLLLAVLLNVR
                     TPLRSLFKAIYYIPVICSGILVGVVWKWIYHGQYGVLNYALSWFGAKPRVWLADTRTA
                     LGALIVVGLWKSVGYYMIIYLAGLQSIPAELYESATIDGASKIQSFFRVTLPMLSPIL
                     LTVLILATIGSFQAFDQVYVMTYGGPVESTSTIVWRMYYTAFIKVQPGEGAAISFLAF
                     LVIMAVSLLQIRYFRSGLLGSDVK"
     gene            complement(2016..2960)
                     /locus_tag="VFH83_02420"
     CDS             complement(2016..2960)
                     /locus_tag="VFH83_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET6485244.1"
                     /translation="MARRITVSTVGPATLTGHPGISGQEAVDVMRAHWTRELAQVLPD
                     RPDLIVLPEMCDRFPEHTPKERRAYYRFRGTAMRDFFAKVAAENRCYIAYSSIRELAD
                     GSWRNSTQILDRRGAVAGVYDKNHVVIEETTDDGILCGRDSPRIETDFGVLSCAICFD
                     LNFEEIRRKTLANRPDLIVFSSMYHGGLMQGFWAYFCRAHFVGAIAGAPPCSVLNPLG
                     EIVAQSTNYYDFVTARINLDCCQVHLDYNWERLAAARLKYGPGVTVRDPGNLGVVLLT
                     SETEGVTALQIAREFEIELWEDYHERALAHRRAHTAGA"
     gene            complement(2994..4121)
                     /locus_tag="VFH83_02425"
     CDS             complement(2994..4121)
                     /locus_tag="VFH83_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET6485245.1"
                     /translation="MREPKDTLDTAAVLAASPLMRGLTSAQLEELVSVSAEQTYAAGE
                     VIVASGVTSTGVHLLVTGEAEVLADGDGGPSATRVALLVPGQVFGELSALTGGPAIST
                     VRAAATPTRVRTFPRSGLEALGDPGDITSTLLRNVITVNQKRLSDSNANYVHQLEQTT
                     ALLLQRQTYARFLFVIILLLGICLFVNHWLAGRSSVDIYSPAFAWAYLVIMVLPTLWV
                     AWRARYPLSLFGLTRRNLGRDLLWSSAILVVVLGLATGALLLGGFPLAQRFNLSYLAR
                     YGPLYSLHSALQEFMGRGVMLGMMLKIFGDSSWKQRQLSNLAVSMMFGLIHIHFGFAT
                     VGLMMLFSLLLGTYYFVHRNLAGPILIHIVLGIAAFMVGIL"
     gene            complement(4118..4873)
                     /locus_tag="VFH83_02430"
     CDS             complement(4118..4873)
                     /locus_tag="VFH83_02430"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6485246.1"
                     /translation="MTEAPTRRGLREDLPVLLQLLRGKPRGLSHADGLNAFYGPQARV
                     YDHFRDRLLPGRQALMTALPLTNGARAVDLGAGTARHWLFVEERLGALARLDLVDLCG
                     PLLQVAADRFRARGNVATHLADAETFRAGAPVDVVVFSYSLTMMEDWQAAVRNACGQL
                     RPDGVVAVVDFYTLPLDPPLPLAALSRWDRWFWPRWFAHDGVRLRPELLPTLLSLGKT
                     LRVEQGRTPVPYLPGFRIPWFLWVGRPERRDPS"
     gene            complement(4870..6135)
                     /locus_tag="VFH83_02435"
     CDS             complement(4870..6135)
                     /locus_tag="VFH83_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtaA family protein"
                     /protein_id="HET6485247.1"
                     /translation="MFLPTLVASCAESASLDWAETPGLAARSSGPLSLHAALFRFLYT
                     RNLLYNTCWEDPAVDRKALRLGESDRLLVITSAGCNALDYALEGPARVVAVDTNPRQT
                     ALLELKLAAIRHLSYEDTFSLFGRGWHVDFPAVYRTALRPELSPFARCFWDSHKHWFS
                     RTTGGLFSHGLCGVVARAVQWRLRAYPALGKAARSLFEQPDLESQRDHYRREVGPRFW
                     SPVLRAALSTRFASSLLGIPAPQRGLVRATGPGVSGSARAFIDDLFCTVPARDNYFWG
                     LYVFGEYPPGCCPRYLTRDGFTRLKEGLVDRIEAHTSTVTRYLKGPGPRFTGAVLLDH
                     MDWMVDHPQALAAEWESLYDRMEPGGRILFRSAHALPPYLEDVRVGPSRLPLREWLSF
                     RTEEARQLARMDRVHTYASFHIAEVRESA"
     gene            complement(6354..7082)
                     /locus_tag="VFH83_02440"
     CDS             complement(6354..7082)
                     /locus_tag="VFH83_02440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET6485248.1"
                     /translation="MERDFWPAGKPAAISFSFDDARLSQVDRGLEILARRDVHATFYV
                     SLPELERRLTGWQQAVRAGHEIGNHSLTHPCSGNYPFARHNPLEEMTLEDMRRELDEA
                     SGRIDQLLGVRPVTFAYPCGNAFVGRGRGTQSYVPLVAERFLAGRGYLNGQTNDPWRC
                     DLSQLVSVGLDRAPSSYARNLIEIALDEGSWLVCTAHDIGDGHDGLSVDVAALEAALS
                     LASERGLWIGTVAEVARHVTEKRG"
     gene            7208..8080
                     /locus_tag="VFH83_02445"
     CDS             7208..8080
                     /locus_tag="VFH83_02445"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6485249.1"
                     /translation="MRIGFAGLGIMGSRMAANLARAGHEMTVWNRSAGKEPAGLAAVR
                     RAHTPAEAAREAEVFITMLATPAAVSTVALGDQGFLGALPRGALWIDSSTVDPSFSRT
                     MAEEAGRRGVRFMDAPVSGSRGPAERGELTFLVGASAEDVVAARPLLERMGKLIVHAG
                     PVSMGAALKLVVNLMLANAMAAFCEALTLGEALGLQRGKILDTLLGGPVTAPVLSAKR
                     ERLEARRYEDADFPLKWMHKDLHLAAAAAYENGVALPVSNAVKELFAMAAETGRGEAD
                     FSAIEDFVSRMRAP"
     gene            8159..9979
                     /locus_tag="VFH83_02450"
     CDS             8159..9979
                     /locus_tag="VFH83_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485250.1"
                     /translation="MSTIVADTKIPIRELARFLGRYLRPRLALVLCLGVTLFSGIGLQ
                     LVNPQLLRSFIDGATAGAPLASLVRLALWYIGIAVAQQLVNVLNVLIAENLGWGTTNA
                     LRLDAARHCLGLDMSFHSGHTPGEMIERLDGDVMALSNFFSRFILEVVGSALLVLGIL
                     LVLLREDWRITVALAGFAGVTSVVCLTLARIAVSSWEAERQSSANLYGFLEERLGGTE
                     DIRANNAVAWVLDRFYRLTRDQMQKTITAGLKVNILLNSMWFAFAVGTALAYLVGSAL
                     YRAGSISIGTVYMIVNYTVMLFWPLDRITQQVQDLQKAIASVARIVGLLRLTGRIPGE
                     GEFAVGQGSRPTAGPLGVSFDRVSFSYDEGGKRETVLRDVSFRLAPGKVLGLLGRTGS
                     GKTTLTRLIFRLYDPEHGGIRLHAGHPGAAPDGVDVRGMAVRDLRRRVGLVPQNIQLF
                     NATVRENLAFFDDSVPDARIVEAIRALGLGPWLDALPRGLDTELESGGGGLSAGEAQL
                     LAFTRIFLRDPGLVILDEATSRLDPATESRIEAAVDTLVRDRTAIIVAHRLRTVQRAD
                     EILILEEGRTLESGTRPELAADPGSRFHRLLETGLEEVLT"
     gene            9976..>11021
                     /locus_tag="VFH83_02455"
     CDS             9976..>11021
                     /locus_tag="VFH83_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485251.1"
                     /translation="MSDARAAGVDRPPPGSWRYFWRMIRYSPVHYATIVALRILIFAV
                     SRQGSGLLQREFFNTFAGTARLGLTAGSIAVLVVALAVGNAGVIFTDIIFHFRYWFRT
                     GALLRKNLLTRILERPGAQAVPGSPGEAISRFRDDANTATDFTLNLPFLIGMALFGVV
                     AVVTMLRISATVTLVAYIPFVLVIVIARWFMKKAEKLREANRSAAGKVTGFIGEIFGS
                     AQAVKAATAESGVLARFERLNQSRRRVAIRDSVFNAALNSIIWNFSSVIAGVILLLVA
                     SRLDARTAGAAHLSLGDFSLFLYYLGFTTELTAVLGMTIAFFKQAGVSVGRMLKLLQG
                     ADPATLVKHSPVYTT"
BASE COUNT         1891 a         3644 c         3671 g         1815 t
ORIGIN      
        1 gcccctctac gaggggaaga tcctggatct caacaagtac ctcaccaagc aggacctggc
       61 ggatttcaag cccgcggcaa tcgcggcttc cacgtacaag ggcgtgctct gctatctgcc
      121 catcctcatc aactccgtct ccatcgtgtt caacatggac gtggtgcaga aggcgggcat
      181 cacggcggac tgggtcaaca ccaagttcct gagctgggac gatttcctga gttactgcgc
      241 caaggtcaag gcaaccggcg cctggccgta caccatgggt ccggcggacg cgggcatcat
      301 cgatgagctc tcgaactgga tgtaccaggc cggcggagac tggtacaacg ccgactacac
      361 gaagtcggcc accaataccc cgcaggccat ccggatgttc aagtacctgg tctccctgtg
      421 ggacaacggg tacatcaacc cggcggataa ggacaagacg gagaccgaga tcgagcaggg
      481 cgtgttcccg gccggtggga tcggggcggt catggtgcag aaccagaaca tcccgaacgc
      541 catcctcaag accaacccgg ccatgcacct ggtctacgcc tatccgttca aggacaaggc
      601 gacgtcctcc aacggcacca tcgccgggta tggctccttc aataccacga agaacccgga
      661 ggcggccgcc gcctggatca aggcgatcac cggtccggag gggatgacca agatcgacaa
      721 ggtggtcaac ttcattccca cccggatctc gctggacgca tccttcaagg acatgatgaa
      781 ggaccccatc ttcaaccggg ccgtggagaa caccgagtgg ttcgtcccga cggttccgcc
      841 gtcaccggtc ggaccggccg cgatcgagga ggtgaagtcc ggcttccaga agatgatcct
      901 gcatcaggcc agcgtggagg acgcggccgc cgacatgacg gcgaggatca acaagcttct
      961 ggacgattac tacaagaaga ccaagtaaac aggggcgcga ccgggccggg ccgtcggcgc
     1021 agagcgccga ccttcgggtc ccggtcgcgt cgttcccagg gccgggaggc atggtgagca
     1081 ggaagaaagg gctcctgtac caggtggtca cgtcgtggcc aagctacgtc ctggtgctgc
     1141 ccgccctcgc gatcctcttc gttttcgaca tctacccgat catcatgacg ctcatcatga
     1201 gcacgcgctt ctacaagaac gtcttcgact cggtctacgt gggactgggc aactaccagt
     1261 acgtgctcac ggactccagc ttctggaacg ccatgaaggt gaccggcatc tacacgctgt
     1321 tcctggttcc cgggctgctc gtcttctccc tgctcctcgc ggtgctgctg aacgtccgca
     1381 cgccgcttcg cagcctcttc aaggcgatct actacattcc ggtgatatgc tccggcatcc
     1441 tggttggagt ggtatggaag tggatctacc acggccagta tggggtgctg aactacgcgc
     1501 tctcctggtt cggcgcgaaa ccccgggtct ggctggcgga cacgaggacg gctctgggcg
     1561 ccctcatcgt cgtggggctc tggaagagcg tggggtacta catgatcatc tacctcgccg
     1621 gcctgcagag catccccgcg gagttgtacg agtccgcaac catcgacggc gcctccaaga
     1681 tccagagctt cttccgcgtc accttgccca tgctctctcc catcctgctc accgtgctga
     1741 tcctggcgac catcggctcg ttccaggcct ttgaccaggt ctacgtgatg acctacggcg
     1801 gacccgtgga gagcacgagc accatcgtat ggcgcatgta ctacacggcg ttcatcaagg
     1861 ttcagcccgg agaaggcgcg gccatcagct tcctggcgtt cctggtgatc atggcggttt
     1921 ccctgctgca gatccgctat ttccgcagcg gcctgctggg atccgacgtg aagtaaaggc
     1981 catcggaggc gccaccgccg agcgcccctg agcgtctatg cgccagccgt gtgcgcgcgg
     2041 cggtgcgcca gggcgcgctc gtggtagtcc tcccagagct cgatctcgaa ctcccgggcg
     2101 atctggaggg ccgtgacccc ctcggtctcc gaggtcagga gcaccacgcc gaggttgccg
     2161 gggtcccgca cggtcacgcc gggaccgtat ttgagccgcg cggcggccag ccgttcccag
     2221 ttgtagtcca ggtggacctg gcagcaatcc aggttgatgc gggccgtcac gaagtcgtag
     2281 tagttggtgc tctgggctac gatctccccc agaggattca gcaccgagca cggcggcgcg
     2341 cccgcgatgg ctcccacgaa gtgcgcgcgg cagaagtagg cccagaaacc ctgcatgagc
     2401 ccgccatggt acatcgagct gaacacgatc agatcagggc gattggccag cgtctttcgc
     2461 cggatctcct cgaagttcag atcgaagcag atggcgcacg acaggacgcc aaagtccgtt
     2521 tcgatgcggg gcgagtcgcg gccgcacagg atcccgtcgt ccgtggtctc ctcgatgacg
     2581 acgtggttct tgtcgtagac gcccgccacg gcgccgcgcc ggtccaggat ctgggtggag
     2641 ttccgccagg agccgtccgc cagctcgcgg atcgaggaat acgcgatata gcagcggttc
     2701 tctgctgcca ccttcgcgaa gaagtcgcgc atggcggtgc cgcggaaccg atagtacgcg
     2761 cggcgctcct tgggcgtatg ctcggggaaa cgatcgcaca tctccggcaa gacgatcagg
     2821 tccggccggt ccgggagcac ctgggccagc tctcgggtcc aatgggcccg catgacgtcg
     2881 acggcctcct ggccgctgat gccagggtgg cccgtcagcg tggccggccc gacggtggag
     2941 accgtgatcc ttcttgccat gagtgcgaac atataccgtg cgcgagaggc cggctacagg
     3001 atgccaacca tgaacgcggc aatccccagc acgatgtgga tcaggatcgg gccggccagg
     3061 ttgcggtgca cgaagtagta ggtgcccagg agcaggctga agagcatcat gaggccaacc
     3121 gtggcgaaac cgaagtggat gtggatgagg ccgaacatca tgctcacggc caggttggag
     3181 agctggcgct gcttccagct cgagtccccg aagatcttga gcatcatgcc cagcatgacg
     3241 ccgcgtccca tgaactcctg gagcgcgctg tgcagagagt acagagggcc gtatctcgcc
     3301 aggtaggaga ggttgaacct ctgcgcgagg ggaaagccgc ccaggagcag agccccggtg
     3361 gccagaccca ggaccacgac aaggatggcg ctgctccaca gaaggtcgcg gcccaggttg
     3421 cgccgggtca atccgaacag cgagagcgga taccgcgcgc gccatgccac ccacagcgtc
     3481 ggcaggacca tgatgaccag gtacgcccag gcaaaggccg gggaatagat gtctacgctg
     3541 gaccgcccgg ccagccagtg gttgacgaag aggcagatgc cgaggagcag gatgatcacg
     3601 aacaggaacc gcgcgtaggt ctgccgttga aggaggagcg cggtggtctg ctccaactga
     3661 tgcacgtagt tggcgttgga gtccgaaagc cgcttctggt tcaccgtgat gacgttgcgc
     3721 aggagggtgc tggtgatgtc cccagggtcg ccgagcgctt ccaggcccga acggggaaac
     3781 gtcctcaccc gggtgggcgt tgccgcggcg cgtaccgtgc tgatggcggg gcccccggtc
     3841 agggcggaaa gctcgccaaa cacctggccg gggaccagca gggccacccg cgtggccgat
     3901 ggccccccgt ccccgtccgc cagcacctcg gcctctccgg tcaccagaag atgtacgccg
     3961 gtgctcgtca ccccggaggc gacgatcacc tctcccgccg cgtacgtctg ctccgcggat
     4021 acagagacaa gctcttcgag ctgagcggac gtgagacccc gcatcagcgg gctggcggcc
     4081 agcacggcgg cggtatccag cgtgtccttg ggctctctca cgacgggtcc cttcgttcag
     4141 gacgacccac ccacaggaac caggggatgc ggaagcccgg gagatagggg accggggtgc
     4201 ggccctgttc cacccgaagc gtctttccca gggaaaggag cgtcggcaac agctcggggc
     4261 gcaacctcac cccgtcgtga gcgaaccatc ggggccagaa ccaccgatcc caccgggaca
     4321 acgccgccag ggggagcggg ggatccaagg gcagggtata gaagtccaca acggcaacga
     4381 ccccgtccgg ccgaagctgc ccacaggcat tgcggactgc tgcctgccag tcctccatca
     4441 tcgtcaagga atacgagaag acgaccacgt cgacgggggc cccggcccgg aaggtctccg
     4501 catcggccag atgggtcgct acattgccgc gagcccggaa tcgatccgcg gccacttgca
     4561 ggagggggcc gcaaaggtcc acgaggtcca gtcgcgccag ggcacccagg cgctcttcta
     4621 cgaacagcca gtgtcgagcc gttcccgcgc caaggtccac tgcgcgcgcc ccgttggtga
     4681 gcgggagcgc ggtcatcagg gcctgccgcc ccgggaggag ccggtcgcgg aagtgatcgt
     4741 agacgcgtgc ctggggtccg tagaaggcgt tcagcccgtc agcgtgggac agtccccgcg
     4801 gcttgccgcg caggagctgc agaagaacgg gcaggtcctc ccggagtccc cgccgcgtcg
     4861 gcgcctccgt catgcgctct cccgcacttc ggcgatgtgg aagctggcgt aggtgtgcac
     4921 gcggtccatc cgcgcgagct gccgcgcctc ctcggtgcgg aaggacagcc actcccgcag
     4981 cggaagacgc gaaggaccga cccgaacgtc ctccagatag ggggggaggg cgtgggcgga
     5041 tcggaagagg atccgaccgc ccggctccat gcgatcgtag agggactccc actccgcggc
     5101 caacgcctgc gggtggtcca ccatccaatc catatggtcc aggagcacgg cgcccgtaaa
     5161 cctgggcccc ggccccttca ggtaccgcgt aacggtggag gtgtgagcct cgatccggtc
     5221 cacgaggcct tccttcagac gcgtgaagcc gtcccgggtc aggtagcggg gacagcagcc
     5281 cggcgggtat tcgccgaaca cgtacaggcc ccagaagtag ttgtcccggg caggcaccgt
     5341 gcagaacaga tcgtcgatga aagcgcgggc ggagccggag acaccggggc cggtggccct
     5401 gaccagaccg cgctggggcg ccgggattcc caggagagag gaggcgaagc gcgtggacaa
     5461 cgccgcccgg agaacgggag accagaaccg gggtccaacc tctctgcggt agtggtcccg
     5521 ctgagactcc aggtccggct gctcgaagag agagcgcgcg gccttcccca gggccgggta
     5581 ggcgcgcagt ctccactgca ccgcccgtgc caccaccccg cacaggccgt gggaaaagag
     5641 cccgcctgtc gtccgcgaga accagtgctt gtgagagtcc cagaagcacc gggcaaacgg
     5701 ggaaagctcc gggcgcagcg cggtccgata gacggccggg aagtcgacgt gccacccccg
     5761 gccgaagagg gagaacgtgt cctcatagga gagatgccgg atggcggcga gcttgagctc
     5821 caggagggcc gtctggcgtg ggttggtgtc caccgccacg acccgggccg gtccttccag
     5881 cgcgtagtcc agcgcgttgc agcccgcgct cgtgatgacg agcagccgat cgctctcacc
     5941 cagtcggagt gccttgcggt caacggccgg gtcttcccag cacgtgttgt agagaaggtt
     6001 gcgggtgtag agaaaccgaa acagggcggc atgcagggag agcggtccgg aggaacgcgc
     6061 ggccaagccg ggggtctccg cccagtccag ggaggcggac tctgcgcagg acgcgacgag
     6121 cgtcggtagg aacacaaggc aatgtatgtt cgcaccaccg gcggcgtcaa ccaaaacctg
     6181 ggtgccatcc gttcgtcgtt cggtgggcga cggaagcgtc cggacccctg cgggagcgac
     6241 tccatcaacc gaacggttga tgcccggccc ccatcttcga ccaaacgctc tatccctgca
     6301 accactccag gtgcgctaga atgggtggac cgagtgcgcg gaaacttcca ccttcagccc
     6361 ctcttctccg tgacatggcg cgccacctcg gctacggtgc cgatccacag gccgcgctcc
     6421 gaggcgaggc tcagggcggc ctccagcgcc gcaacgtcca ccgagaggcc gtcgtgtccg
     6481 tcgccgatgt catgcgcggt gcagacgagc caggagcctt cgtcgagcgc gatctcgatc
     6541 aggttccgcg cgtacgacga gggcgcgcgg tcgagcccga cggagaccag ctggctcagg
     6601 tcgcagcgcc acgggtcgtt cgtctggccg ttgaggtagc cgcgtcccgc aaggaaacgc
     6661 tccgccacga ggggcacgta ggactgcgtc ccccgtcccc gtccgacgaa ggcgttgccg
     6721 cacggatagg cgaacgtcac cggccgcacg ccgagcagct ggtcgatcct gcccgaggct
     6781 tcgtccagct cgcgtctcat gtcctccagc gtcatctcct ccagcgggtt gtgacgcgca
     6841 aagggatagt tgccgctgca agggtgtgtg aggctgtggt tgccgatctc gtgacccgcg
     6901 cgcaccgcct gctgccatcc ggtcaggcgc cgctccagct cgggcaggga gacgtagaac
     6961 gtggcgtgaa cgtcgcggcg ggcaaggatc tcgagccctc gatccacctg gctgaggcgg
     7021 gcgtcgtcga aggaaaagct gatcgcggcc ggttttccgg ctggccagaa gtccctctcc
     7081 atggtggcgc cactgtacgg cgaacgcgcc gctccgacaa gccggccggt cgtttgggtg
     7141 acccagagcc gtcctaccgc gtgccggcgg cgcggcttcg gagtatcttg gcctcaaagg
     7201 agagatcatg cgcatcggat ttgccggtct ggggatcatg ggcagcagga tggcggccaa
     7261 cctggctcgg gctggccacg agatgaccgt ttggaaccgg tccgcgggca aggagcccgc
     7321 cggtctcgcg gcggtccgtc gggcgcacac acccgcagag gcggcccggg aggctgaagt
     7381 ctttatcacc atgctggcaa ctcccgcggc ggtgtccacg gtcgcccttg gcgatcaggg
     7441 cttcctcgga gctctgcccc gcggggctct gtggatcgac tcgagcaccg ttgacccgtc
     7501 gttctcccgg acgatggcgg aagaggccgg ccgtcgtggc gtccgtttca tggacgctcc
     7561 cgtctccggt agccgagggc ccgccgagcg aggcgagctc acgttcctgg tgggcgcctc
     7621 ggccgaggac gttgtcgcgg caagacctct gcttgagcgg atggggaaac tgatcgtgca
     7681 cgccggcccg gtcagcatgg gagccgccct gaagctggtg gtgaacctga tgctggccaa
     7741 cgccatggca gcgttctgcg aggcgctcac gctgggagag gccctgggtc tgcagcgagg
     7801 caagatcctg gacacgctcc tgggaggtcc ggtgaccgcg ccggttctct cggcaaaacg
     7861 cgagcggctg gaggcccgcc ggtatgaaga cgccgacttt cccctaaagt ggatgcacaa
     7921 ggaccttcac ctcgccgcgg ccgcggccta tgagaacggg gtggccctac cggtgtccaa
     7981 cgcggtcaag gagctgttcg ccatggccgc ggagaccggc cgaggagagg ctgacttctc
     8041 ggcgatcgag gactttgtga gccgcatgcg agccccatag cccgccgcaa ccaccgcgcc
     8101 cctccgcgct tgattcctcg gtgctccgcg tctcatactg acggcagaac gaagtcctat
     8161 gtccacgatc gttgcggaca cgaagatccc catccgggag ttggcgcggt ttctgggccg
     8221 gtacctcagg ccccggctcg cgctggtgct ctgcctcggc gtgaccctct tcagcgggat
     8281 cgggctgcag ctcgtcaacc ctcagcttct gcgctcgttc atcgatgggg ccaccgcggg
     8341 ggcccccctg gcgtccctgg tccgtctggc gctgtggtac atcgggatcg cggtcgccca
     8401 gcagctggtc aacgtcctga acgtgctcat cgcggagaac ctgggatggg gcacgaccaa
     8461 cgcgctgcgc ctggatgccg cccgtcactg tctgggactg gacatgagct tccactcggg
     8521 acacacgccg ggggagatga tcgagcggct ggatggggac gtgatggcgc tctccaactt
     8581 cttctcccgg ttcatcctgg aggtcgtcgg aagcgctctc ctggtgctgg gcattctgct
     8641 ggtccttctt cgggaggact ggcgcattac cgtcgcgctc gccggctttg cgggggtcac
     8701 gtcggtcgta tgcttgacgc tggcgcgcat cgccgtctcc agctgggagg cggagcggca
     8761 gtcgagcgcc aacctctacg gctttctgga agagcgcctg ggcggcacgg aggacatccg
     8821 ggccaacaac gccgttgcct gggtcctgga ccggttctac cgtctcaccc gcgaccagat
     8881 gcagaagacg atcacggccg gcctgaaggt caacatcctc ctgaactcca tgtggttcgc
     8941 gttcgcggtg gggacggccc tcgcgtacct cgtcggatcc gcgttgtatc gggccggttc
     9001 gatcagcatc gggaccgtgt acatgatcgt caactacacc gtgatgctgt tctggcctct
     9061 ggaccgcatt acccaacagg tgcaggacct gcagaaggcc atcgcgagcg tggcccggat
     9121 cgtcggtctg ctgcgcctga ccggcaggat ccccggggaa ggggagttcg cggttggcca
     9181 gggatccagg cccaccgccg gaccgcttgg cgtctccttc gatcgggtct ccttctccta
     9241 cgacgaaggc ggcaagcggg aaaccgtgtt gcgcgacgtc agcttccgtc tggcgccggg
     9301 caaggtgctg ggtctcctgg gccgcaccgg cagcgggaag accacgctca cgcggctgat
     9361 cttccgtctc tacgatcccg agcacggggg catccgtctt cacgccggtc atccgggcgc
     9421 ggctcccgac ggggtagacg tccgcggcat ggcggtgcga gacctgcgcc gtcgcgtggg
     9481 cctggtgccg cagaacatcc agctcttcaa cgcgaccgtg cgcgagaacc ttgcgttctt
     9541 cgacgactcg gtgcccgacg cgcgtatcgt agaggccatc cgcgcgctgg gactggggcc
     9601 ctggctggat gcgctcccgc gcggcctgga caccgagctg gaatccggtg gcggggggct
     9661 ctccgcggga gaggcgcagc tcctggcctt cacgcgcatc ttcctgcggg accccggcct
     9721 ggtgatcctg gacgaggcga cgtcccggct ggacccggcc accgagtccc ggatcgaggc
     9781 ggcggtggac accctggtgc gggaccgcac ggccatcatc gtcgcgcacc gcctgcgcac
     9841 cgtgcagcgc gccgacgaga tcctgatcct ggaggagggc cggacgctcg agagcggaac
     9901 ccgtccggag ctggcagcgg atccgggctc ccggttccac cggttgctgg agaccggtct
     9961 ggaggaggtg ctgacgtgag cgacgcccgt gccgccggcg tcgaccgtcc gcccccgggc
    10021 tcctggcgct acttctggcg gatgatccga tactctcccg tccactacgc gacgatcgtc
    10081 gcgcttcgca tcctgatctt cgcggtctct cgccaggggt ccggtctgct tcagcgcgag
    10141 ttcttcaaca ccttcgccgg gaccgcgcgc ctgggcctga ccgccgggtc catcgcggtg
    10201 ctggtggtgg cgctggccgt cggcaacgcc ggagtcatct tcacggacat catcttccat
    10261 ttccgctact ggtttcgcac cggcgccctc ttgcggaaga acctgctcac ccgaatcctg
    10321 gaacggcccg gcgcccaggc ggtaccgggc tctcccggag aagcgatcag ccggttccgc
    10381 gatgacgcca acaccgccac ggacttcacg ctcaatctac ccttcctgat cggcatggcc
    10441 ctcttcggtg tggtggccgt ggtcaccatg ctgcgcatca gcgccaccgt caccctcgtg
    10501 gcctacatac ccttcgtgct ggtgatcgtg attgctcgct ggttcatgaa gaaggcggag
    10561 aagcttcggg aggccaaccg gagcgcggcc ggaaaggtca ccggcttcat cggggagatc
    10621 ttcggctctg cccaggcggt gaaggcggcg accgcggaat ccggggttct ggcgcgcttc
    10681 gagcgcctga atcagagccg ccggcgggtc gccatccgtg acagcgtgtt caacgcggcg
    10741 ctcaactcca tcatctggaa cttctccagc gtcatcgccg gcgtcatcct gctcctggtc
    10801 gcctcgcggc tggacgcgcg gaccgccggc gcggcccacc tcagcctcgg cgacttttcc
    10861 ctgttcctgt attacctcgg cttcactacg gagctgaccg cggtactggg catgacgatc
    10921 gccttcttca agcaggccgg cgtctccgtg ggccgcatgc tcaagctcct gcagggcgcc
    10981 gaccccgcga ccctggtgaa gcacagcccc gtatacacca c
//
LOCUS       DASRZS010000102         3791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3519563, whole genome shotgun sequence.
ACCESSION   DASRZS010000102 DASRZS010000000
VERSION     DASRZS010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3519563"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFH83_02460"
     CDS             <1..911
                     /locus_tag="VFH83_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6485252.1"
                     /translation="MVYGIVKLINFAHGDVFMVGAFTSFFAVDQFHLQQWPSLLFPAI
                     APAVSIVVGSLTVILLSMLICLLLAVTIERVAYKPLRDAPRIAALITAIGVSFFLEYF
                     GQLQFVYTARFIPYHSPFDTLSWYIQDGIRPVVQGQPVPDNTVTFSNIFLLIVAASVL
                     VQLFLQFLVRRTKIGIAMRAASFDKPTARLMGINVNAVISFTFATGAAFAGLGGVLYA
                     IAYPSIWTLLGITPGLKAFVAAVLGGIGSLPGALVGALIMGQAENLTASYVSTPMRDV
                     VAFAILIIVLLVRPTGLFGEPSGEKA"
     gene            921..2291
                     /locus_tag="VFH83_02465"
     CDS             921..2291
                     /locus_tag="VFH83_02465"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6485253.1"
                     /translation="MELGYLFTTIVKMAALLAWIFGLLWGLARKQLWQKALTLLLAFG
                     IIQVAFLSFGDFSLFTGFDEGLLFQAISMTMVALGLNLIYGFNGQFSLAQWGFYGLGA
                     YCAADITYRWTNGDASGLFVVGIGVILGGLALVGVGRLLRLRRGIPVLSAFTFYLLAI
                     VAAVAVAVVLGKALAGAIDPMFGTSRSPGVLTSPAVVQIVFVLSVILAGAFAAGVSFL
                     FGLPVLTLGSDYFGIATLGFAIVVQTLMVNTDTLLPFPEMKGARGMIGIPKLTSWIAA
                     FAFLVVVIIIVRNFVRSSTGRAALAVREDEVAAKAMGVDVAGSKLLAFVVGSFVAGIG
                     GGVYAHYLAFLSPSTFSFINGFNPLIIVVFGGLGSMTGTITAAFAWIFFLEGFLRVLL
                     GQMGTSAPAWRFVLYPVTLLLVMLIRPQGLLGSVEWGFLKPDQFKRRLAPQEPPVAAV
                     AGKGAS"
     gene            2293..3093
                     /locus_tag="VFH83_02470"
     CDS             2293..3093
                     /locus_tag="VFH83_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020753763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485254.1"
                     /translation="MAEPTTNVIEIAELTKFFGGLCAVKGFSLSVPQGALWGLIGPNG
                     AGKTTVFNLLTGLIPLSSGEIRFRDERIDGLESFEITRMGIARTFQNIRLFSNLSVLD
                     NVRIAYHAHAGYSLSDSLLRTRKFLGREKDLTEQALDFLSIFKLDGIAEETAKNLPYG
                     EQRRVEIARALACNPALLLLDEPAAGMNPREIVQLMELIHFIRDRFKLTILLIEHQMR
                     VVMGICEFITVMDFGEVIARGTPKEIQGNQTVIEAYLGKGVASGAAGR"
     gene            3077..3781
                     /locus_tag="VFH83_02475"
     CDS             3077..3781
                     /locus_tag="VFH83_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008539876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485255.1"
                     /translation="MLQVDNLNVYYGAIHALQGISFNVNEGEIVTLIGANGAGKSTTL
                     RTISGLLRSRTGSITFQGADISLVPAERIVRMGISHVPEGRKIFAPLSVRENLMMGAF
                     TRDNQGEIQETLQRVFRSFPRLKERVGQYGGTLSGGEQQMLATARGLMSRPSLLLMDE
                     PSMGLSPILVEEIFRIITEINKEGTSILLVEQNAQMALSIAHRAYVLETGRIVLSGSA
                     KEIADNSQVKAAYLGL"
BASE COUNT          586 a         1243 c         1183 g          779 t
ORIGIN      
        1 ccatggtgta cgggatcgtg aagctcatca acttcgccca tggggacgtg ttcatggtgg
       61 gcgccttcac gagcttcttc gcggtggatc agttccacct ccagcagtgg ccgtccctgc
      121 tcttccccgc catcgccccc gcggtctcga tcgtcgtggg ctccctcacg gtcatcctgc
      181 tctccatgct catctgcctg ctcctggcgg tgaccatcga gagagtggct tacaagcccc
      241 ttcgggatgc cccccggatc gcggccctga tcacggccat cggcgtctcg ttcttcctcg
      301 agtatttcgg tcagctccag ttcgtctata cggcccgttt catcccgtat cacagcccct
      361 tcgacacgct gtcctggtac atccaggacg ggatccgccc cgtggtccag ggccagcccg
      421 tgccggacaa cacggtgacc ttctcgaaca tcttccttct catcgtggcg gcttccgtgc
      481 tggtccaact cttccttcag ttcctggtcc gccggacgaa gatcggcatc gccatgcggg
      541 ccgcatcctt cgacaagccg acggcccgcc tcatgggcat caacgtcaac gctgtgatct
      601 ccttcacctt cgccaccggc gcggcctttg cggggctggg cggggttctg tacgccatcg
      661 cctatccgtc catctggacg ctgctgggga tcacgcccgg actcaaggcg ttcgttgccg
      721 cggtgctcgg cgggatcggc agcctccccg gcgcccttgt gggcgccctg atcatgggcc
      781 aggcggagaa cctcacggcg agctacgtct ccactccgat gcgcgacgtg gtcgcgtttg
      841 cgatcctcat cattgtgctt ctggtgcggc ccaccggtct cttcggggag ccctccgggg
      901 agaaggcgta gggggagccc atggaactgg ggtatctctt cacgacgatc gtcaagatgg
      961 cggccctgct cgcctggatc tttggtctgc tctggggtct ggcccgcaaa caactctggc
     1021 agaaagcgct gaccctcctc ctggccttcg ggatcatcca ggtggcgttc ctctcgttcg
     1081 gtgatttcag tctcttcacc ggctttgacg aaggcctcct cttccaggcc atcagcatga
     1141 ccatggtggc gctgggcctg aacctgatct acggtttcaa cggtcagttc agcctggccc
     1201 agtggggctt ctacggtctt ggcgcgtact gcgccgcgga catcacctac cgttggacca
     1261 acggagacgc cagcgggctg ttcgtggtag ggatcggggt gatcctgggc ggccttgcgc
     1321 tggtcggcgt gggaaggctc cttcggctgc gcagaggcat cccggtcctc tcggcgttca
     1381 ccttctacct ccttgccatt gtggccgcgg tcgccgtggc ggtggttctc ggaaaggccc
     1441 tggcgggcgc catcgatccg atgttcggca cctcccgaag cccgggcgtt ctgacgagcc
     1501 cggcggtcgt ccagattgtg ttcgttctgt cggtgatcct ggccggcgct ttcgccgcgg
     1561 gtgtgagctt cctgttcggt ctgcccgttc tcaccctggg aagcgactat ttcggaatcg
     1621 ccacgttggg gttcgccatc gtggtgcaga ccctcatggt caacacggac acgctgctgc
     1681 cgttccctga gatgaagggg gcgcgcggga tgatcggcat ccccaagctc acctcatgga
     1741 tcgccgcgtt cgcgttcctg gtcgtggtca tcatcatcgt ccggaacttc gttcgctcca
     1801 gcacgggcag ggcggctctg gcggtgcggg aagacgaggt cgccgccaag gccatggggg
     1861 tggacgtggc gggctccaag ctcctggctt tcgtcgtggg cagcttcgtc gctggcatag
     1921 ggggcggcgt gtacgcgcac tacctggcct tcttgagccc cagcaccttc agcttcatca
     1981 acgggttcaa cccgctcatc atcgtggtgt tcggcggtct ggggagcatg acggggacca
     2041 tcacggccgc gttcgcgtgg atcttcttcc tggaagggtt cctgagggtc ctgctgggac
     2101 agatggggac gtccgcgccg gcgtggcgct tcgtcctcta ccccgtgacg ctgcttctgg
     2161 tcatgctgat ccgtccccag gggcttctgg gctccgtgga gtgggggttc ctgaagccgg
     2221 accagttcaa gcggcgcctg gcgccgcagg agccacccgt tgccgccgtg gcggggaaag
     2281 gggcgagctg acatggcgga gcccaccacc aacgtcatcg agatcgccga gctcaccaag
     2341 tttttcggcg gtctgtgcgc tgtgaaggga ttctccctct ccgttcccca gggggccctc
     2401 tggggcctga tcggacccaa cggcgccggc aagacaacgg tgttcaacct gctcaccggt
     2461 ctgatccctc tgtcctcggg ggagatccgc ttccgtgatg agcggatcga cggactggag
     2521 tcattcgaga tcacgcggat gggcattgcc cgcaccttcc agaatatccg gctcttctcc
     2581 aatctttcgg tgctggacaa cgtgcgcatc gcctaccacg cccacgccgg ctattcgctc
     2641 tccgacagcc tcctgcggac ccggaagttt ctgggtcggg agaaggatct caccgaacag
     2701 gccctcgatt tcctttccat tttcaagctg gacggcatcg cggaggagac cgcgaagaac
     2761 cttccctacg gagagcagcg acgcgtggag atagcccgag ccctggcctg caatccggcg
     2821 ctcctgcttc tggacgagcc ggcggccggc atgaaccctc gtgagatcgt ccagctcatg
     2881 gagctcatcc atttcatccg ggaccggttc aagctcacca tcctcctgat cgagcaccag
     2941 atgcgcgtcg tgatgggcat ctgcgagttc attacggtca tggacttcgg agaggtgatt
     3001 gcccgcggga cgcccaagga gatccagggc aaccagacgg tcatcgaggc gtatctcggc
     3061 aaaggagtgg ccagcggtgc tgcaggtcga taacctcaac gtctactacg gggccatcca
     3121 cgcgctacag ggcatctcct tcaatgtcaa cgagggggag atcgtcaccc tgatcggcgc
     3181 caacggcgcc ggcaagtcca ccaccttgcg caccatctcc ggactgctgc ggtcccgcac
     3241 cggctcgata accttccagg gggcggacat ctcccttgtc cccgcggagc gaatcgttcg
     3301 aatggggatc agccacgtcc ccgagggccg caagatcttc gctcccctct ccgtccggga
     3361 gaacctcatg atgggcgcgt tcacccggga caaccagggg gagatccagg agaccctcca
     3421 gcgcgtgttc cgaagctttc cccggttgaa ggagcgcgtg ggacagtacg gcggcaccct
     3481 ttccggcgga gagcagcaga tgctggcgac ggcccgaggg ctcatgagtc ggcccagcct
     3541 cctgctcatg gacgagccct ccatggggct ctccccgatc ctggtggagg agatcttccg
     3601 catcattacg gagatcaaca aggaagggac cagcatcctc ctggtggagc agaacgcgca
     3661 gatggcgctg tccatcgctc accgcgccta cgtgctggaa acgggccgga tcgttctctc
     3721 gggaagcgcg aaggagatcg cggacaattc tcaggtgaaa gctgcctatc tgggcctgta
     3781 agcggggcgc g
//
LOCUS       DASRZS010000103         4987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_445228, whole genome shotgun sequence.
ACCESSION   DASRZS010000103 DASRZS010000000
VERSION     DASRZS010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445228"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFH83_02480"
     CDS             <1..307
                     /locus_tag="VFH83_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485256.1"
                     /translation="ERMNPEDKSKLQSLVSDLSGLRSRTPEESKFKDWKEKAEKKVEE
                     LFGKSSDQYSRFRSLKFFDFARRSGMPKDAPLREDERSQFLKGLEDARRLLSRFMDY"
     gene            460..2373
                     /locus_tag="VFH83_02485"
     CDS             460..2373
                     /locus_tag="VFH83_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6485257.1"
                     /translation="MDQTVVQVHWSVAEKYPDSIALMYKNRKGDWESVPFREFARLYE
                     RFGAGMLDFGVERGDHVGIISDNRKEWIIANLGILGLGAADVPRGSDTMPDEVGYILK
                     HSDCAVVLAENADQLRKILSHKKELPHLKTVIVIDEAFKPAAFKEPTLRVKLVRFADI
                     MDRGKKLLEKDPEAWQREMRKGKPGDLATLIYTSGTTGEPKGVMLTHGNFLHNLRTIP
                     AAIDVGPGDLFLSVLPVWHSFERIIENFALSRGATMAYSKPIGKVMMADMAAVKPTVM
                     GSIPRLWEVVRAAIYRKVNEEGGVKKALFQFFVAAGKAHATSTALVRGLYPQFTRRYR
                     ALDFILGVFPFLLLYPIRGLGNLLVFGKVKQQLGGRFRFTVSGGGALPPYVDKFFGAA
                     GVLLLEGYGLTETTPVVSVRLQKHPVPGTIGPLLPEQQVRLIDPETGAEVGPGKKGVI
                     YLKGPNVMKGYYKRPKVTAEVLSPDGWLNTGDLGMLTYQGELRIIGRTKETIVLLGGE
                     NVEPAPIEDTILESDYIDQVMVIGQDQKFLGALVVPNQEALEKYAKGRGLSWAKVDEL
                     LEHPQVLDLITAEIQERINAKRGFRGFERVFRFKMIPKHFEPGVELSGNLKMKRNVIS
                     EIYKRQISEVFAK"
     gene            2406..4034
                     /gene="bshC"
                     /locus_tag="VFH83_02490"
     CDS             2406..4034
                     /gene="bshC"
                     /locus_tag="VFH83_02490"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HET6485258.1"
                     /translation="MFQSIALRDLPMTSALLADCWEGASRFRSLVPRHLGDPRSFGEL
                     ARLLEARRYDRAGLAQVLTEQNQAFGAGAEALRAAGRLRDPSAVIVVTGQQAGLFGGP
                     LYTVNKALTVLGLAARLESQLGRPVVPVFWIASEDGDLAEVDRASILDREGNIQELRL
                     PGDEGARMPVSLVRLGEEVRELLSATSAFLPETGYTEEMLSDLRSAYAPGRSYPQAFG
                     AWMASLFQGRPLVLLDPSDTRLKRMAAGLFRREILEGSPVSLAVREQTAAVQKAGYPA
                     QIELRDGLLTLFYQNPARESLASWAEGWEIKDKNHRFRSAELADLADREPERLSPNAV
                     LRPLFQDTILPTVAVVLGPAELAYWTQLPLAYQRFGIPMPVLVPRASLTVIDARMHKL
                     LGRLELTLPDLLRRGERVLDDLVRRRIPPETFERLADARGQVGRLWEPIIADANRLDP
                     TLKPTASLASARALRQIDWVEKKLVQAARRKDELLRRQVHQVVSALAPRGGLQERTLT
                     VLPFLARAGRGLLDQMEAAVDPFAPEHRAVVLES"
     gene            4071..4793
                     /gene="bshB1"
                     /locus_tag="VFH83_02495"
     CDS             4071..4793
                     /gene="bshB1"
                     /locus_tag="VFH83_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HET6485259.1"
                     /translation="MTVDILAFGAHADDVELAASGTLMRAAAGGARTGVITLTRGEMG
                     TRGTPELRAREFDEAAEAMGLAHHEMLSLPDGRLAPTEEARQLIVREIREHRPRIVLL
                     PYPEDRHPDHPAASKIIQDAAFLAGLRKLETGQEPHRPAELIYYMPSWEFEPSFIVDI
                     SDYIERKSAVLKAYASQVYHPEQAGAWEPTFIASEHFMKLLFARMAHYGRLIGKAYGE
                     PFWIRSKIEISDLLAAFGGRTY"
     gene            complement(4818..>4987)
                     /locus_tag="VFH83_02500"
     CDS             complement(4818..>4987)
                     /locus_tag="VFH83_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019812641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET6485260.1"
                     /translation="LQGYYVWSLLDNFEWAMGYSKRFGIVSVDYHTQQRTWKASAGWY
                     QRVMASNGAEL"
BASE COUNT          883 a         1606 c         1658 g          840 t
ORIGIN      
        1 ggaacgcatg aacccagagg acaagtccaa gctgcaatca ttggtgagcg acctgagcgg
       61 tctgcgctcc cggacccctg aggagtcgaa gttcaaggac tggaaggaaa aggctgagaa
      121 aaaggtcgag gagctcttcg gcaagagctc ggaccagtac agcaggttcc gatccctgaa
      181 gttcttcgac ttcgcgcggc gctcggggat gcccaaggac gcgccgctgc gggaagatga
      241 gcgctcccag ttcctgaagg ggctggagga cgcgcgccgg cttctatccc ggttcatgga
      301 ctactgactt cggcggggcg cgccccgaaa tggcggcttt ttcgccacgg attatccgtc
      361 gaaccggtac taaacttgac cgcaggaatg ggccatgcta ctcttggtgc gcctcattcc
      421 ttatcctagc caccgccatt tgacgaacag ggggaagaca tggaccagac cgtcgtccag
      481 gtgcactggt cggtagccga gaagtacccg gactcgatcg ctctcatgta caagaaccgc
      541 aaaggggact gggaatcggt cccgttccgc gagtttgccc gcctgtacga gcgcttcggg
      601 gctgggatgc tcgacttcgg cgtcgagcgg ggggatcacg tagggatcat ctcggacaac
      661 cgcaaggaat ggatcatcgc caacctgggg atcctgggtc tgggcgctgc ggacgttccc
      721 aggggctccg ataccatgcc ggacgaggtc ggctacatcc tgaaacactc ggactgcgcc
      781 gtggtcctgg cggagaacgc ggatcagctt cgcaagatcc tttcgcacaa gaaggagctt
      841 ccccacctca agaccgtcat cgtcatcgac gaagccttca agcctgccgc gttcaaggag
      901 ccgaccctcc gggtgaagct cgtccggttt gccgacatca tggatcgggg caagaagctc
      961 ctggaaaagg atccggaggc ctggcagcgc gagatgcgca agggaaagcc tggggatctg
     1021 gccaccttga tctacacctc cgggaccacg ggggagccga agggcgtcat gctcactcac
     1081 ggcaacttcc tccacaacct gcggaccatt cccgccgcca tcgatgtcgg accgggggac
     1141 ctctttctct cggtgctccc cgtctggcac tccttcgagc gcatcatcga gaacttcgcc
     1201 ctgtcgcggg gcgccaccat ggcgtattcc aagcccatcg ggaaggtcat gatggcggac
     1261 atggccgcgg tgaagccgac cgtcatgggg tccatcccgc gactctggga agtcgtgcgg
     1321 gcggccatct accgcaaggt gaacgaggaa ggcggcgtca aaaaggcgct cttccagttc
     1381 ttcgtcgcgg ccggcaaggc acacgccacc agcaccgccc tggtccgggg cctgtatccc
     1441 cagttcacgc ggcgctaccg ggcgctggac ttcatcctgg gtgtctttcc gttcctgctc
     1501 ctgtacccga tccgaggtct cggcaacctg ctggtcttcg gcaaggtaaa acagcagctc
     1561 ggcggacggt tccgtttcac cgtctcaggc ggaggcgctc tgccgccgta cgtggacaag
     1621 ttcttcggcg ctgccggcgt cctcttactg gaaggctacg ggctcaccga gaccacgccg
     1681 gtggtttccg tgaggcttca gaagcacccc gtgcccggga ccatcggccc gctcctgccg
     1741 gagcagcagg tcaggctcat cgatccggag acgggggccg aagtgggtcc cggcaagaag
     1801 ggcgtcatct atctcaaggg ccccaacgtg atgaagggct actacaagcg gcccaaggtc
     1861 accgcggaag tcctttcccc cgacggatgg ctcaacacgg gagacctggg aatgctcacg
     1921 taccaggggg agctgaggat catcggcagg accaaggaga ccatcgtgct tctgggcggt
     1981 gagaacgtgg agccggcgcc catcgaggac acgattctgg agagcgacta catcgaccag
     2041 gtcatggtga tcggccagga ccagaagttc ctgggtgccc tggtggtgcc caaccaggaa
     2101 gccctggaga agtacgccaa gggtcgcggc ctgagctggg ccaaggtcga cgagcttctg
     2161 gagcaccccc aggtcctgga cctgatcacc gcggagatcc aggagcggat caacgccaaa
     2221 cgcgggttcc gcgggttcga gcgcgtgttc cggttcaaga tgatcccgaa gcacttcgag
     2281 cccggcgtcg agctgagcgg caacctcaag atgaagcgca acgtgatcag cgagatctac
     2341 aagcggcaga tctcggaagt tttcgcgaag tagatcgaac cggctctttc agggtaggat
     2401 gactgatgtt ccagtccatc gcgctgcgcg atctgcccat gaccagcgcg ctcctggcag
     2461 actgttggga aggagcgtcc cggttccgat cgttggtccc tcgacacctc ggtgatccac
     2521 ggtccttcgg agagctggcg cggctgctcg aggcgcgacg ctacgaccgc gcggggctgg
     2581 cgcaggtcct cacggagcag aaccaggcgt tcggggccgg cgcggaagcc ctgcgggcgg
     2641 ccggtcggct gcgggatccg tccgcggtga tcgtggtcac gggacaacag gccgggttgt
     2701 tcggcgggcc gctgtatacg gtgaacaagg cgttgacggt gctcggcctg gcggcgcggc
     2761 tggagagcca actgggtcgc cccgtagtgc cggtgttctg gatcgcctcc gaggatggtg
     2821 accttgccga ggtggatcgc gcgtccatcc tcgatcggga agggaacatc caggagttga
     2881 ggcttccggg tgacgagggt gcccgcatgc cggtctccct cgtgcggctg ggagaggagg
     2941 tccgggagct cttgagcgcc acgtcggcgt tccttcccga aacgggctat acggaggaga
     3001 tgctctccga tctgcgatcc gcttacgctc ccggccgcag ctacccgcag gcgttcggcg
     3061 cctggatggc ctccctcttc cagggccgtc ccctcgtcct gctggatccc tccgacaccc
     3121 gcctgaaacg aatggccgcg gggctcttcc gccgggagat cctggagggc agcccggtaa
     3181 gcctggcggt ccgggagcag acggccgcgg tgcagaaagc cggctatccg gctcagatcg
     3241 agctccgcga tggcctgctc acccttttct accagaaccc cgcgcgggag agcctcgcct
     3301 cctgggcgga aggatgggag atcaaggaca agaaccacag gttccgatcc gccgagcttg
     3361 cggacctggc ggaccgcgag ccggagcggc tcagccccaa cgccgtgctg cggccgctgt
     3421 tccaggacac catcctgccc acggttgccg tggtgctggg cccggcggag ctcgcctact
     3481 ggacgcagct cccgctggcg tatcagcgtt tcgggatccc gatgccggtc ctcgtccctc
     3541 gagcctccct gaccgtgatc gacgcccgga tgcacaagct cctgggaagg ctcgagctca
     3601 cgctgccgga tctccttcga cgcggcgagc gcgtcctgga cgacctggtc cgacgacgga
     3661 tcccgccgga aaccttcgag aggctggcgg acgcccgtgg ccaggtgggc cggctttggg
     3721 agcccatcat cgcggacgcg aaccggctgg acccgacgct gaagcccacg gcgtccctgg
     3781 cctcggcccg cgcgctccga cagatcgact gggtggagaa aaagctcgtc caggcagccc
     3841 gacgcaagga cgagctcctg aggcgccagg tccaccaggt ggtgtcggcg ctggcgcccc
     3901 gaggcggcct ccaggagcgg actctgacgg tgcttccgtt cctggcgcgc gccgggcgag
     3961 gccttctgga ccagatggag gcggccgtgg atcccttcgc gccggagcat cgggccgtgg
     4021 tgctggaatc atgagcgcgg tgatcgagtc atgaccgcgg cggtgggtcc atgaccgtgg
     4081 atatcctggc gttcggagcg cacgcggatg acgtggagct tgcggccagc ggcacgctga
     4141 tgagggcggc ggccggaggc gcgcgcacgg gcgtcatcac cctcacccgt ggagagatgg
     4201 ggacccgagg gacccccgag ctgcgcgcgc gggagttcga cgaagccgcc gaggccatgg
     4261 gtctggcgca tcacgagatg ctgtctctgc cggacggccg gcttgccccg accgaggagg
     4321 cccgtcagct catcgtccgc gagatccggg agcatcgacc gcgcatcgtt cttctgccct
     4381 accccgagga ccggcacccc gatcacccgg ccgcgtccaa gatcatccag gacgccgcgt
     4441 tcctggccgg cctgcgcaag ctggagaccg gccaggagcc tcatcgtccg gccgagctga
     4501 tctactatat gccgagctgg gagttcgagc cgagcttcat cgtggacatc agcgactaca
     4561 tcgagcgcaa gagcgcggtc ctcaaggcgt acgccagcca ggtctaccac cccgagcagg
     4621 caggggcatg ggagccgacc ttcatcgcca gcgagcattt catgaagctg ctctttgcgc
     4681 gcatggccca ctacgggcga ctcatcggca aggcgtacgg agagcctttc tggatccgca
     4741 gcaagatcga gatcagcgat cttcttgccg ccttcggcgg acggacgtac tagcctgcct
     4801 gggccgccgc gccttcccta cagctccgcg ccgttcgatg ccatgacccg ctgataccag
     4861 ccggcgcttg ccttccaggt tctctgctgg gtgtggtagt ccacggaaac gatcccgaac
     4921 cgtttggagt agcccatggc ccattcgaag ttgtccagga gcgaccagac gtaatagccc
     4981 tggagcc
//
LOCUS       DASRZS010000104         4572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_445831, whole genome shotgun sequence.
ACCESSION   DASRZS010000104 DASRZS010000000
VERSION     DASRZS010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445831"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..998
                     /locus_tag="VFH83_02505"
     CDS             303..998
                     /locus_tag="VFH83_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6485261.1"
                     /translation="MARTVKPAEHEAKRNEILDAAAGLVYSKGYEKMTIQDLLDTLRI
                     SRGALYHYFVSKQQLVEALIERSAGDMERSLLAIVQDPGLSAVEKIQGYFDIATRWKT
                     SQKDLIMGALRSWYSDDNVLLRQKLGEKSRRYVSRLLEPIIHQGVQEGLFATPYPAEI
                     AAIFAGMTLNLSDCLVELMLAEPPEPGTLRRAQALLDAYTVSIERILGAGAGSFRPVN
                     FGVFKDWFSPERG"
     gene            1035..1907
                     /locus_tag="VFH83_02510"
     CDS             1035..1907
                     /locus_tag="VFH83_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015592409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485262.1"
                     /translation="MSVIEVADLTKFYGKARGIVDVSFRQEEGEIFGFIGPNGAGKST
                     TIRLLLSLIHPTSGSARIFGKDVVSEGPRIRRDIGYLPSEVFYYEGMKVIDLLKYSAS
                     FFGGNRTRRIQELSDTMELELGRRISDLSYGNRKKVGIVQGLLHSPKLLFLDEPTSGL
                     DPLMQRRFFDLIRKENERGVTVFFSSHILGEVQRMCNRVGIIREGRIVEISDIRALQQ
                     STYKKVNVSGDHLQPAAFRLPGVEQLEEEDGSLRFFFKGDINALVRAMSTVRVSNVTI
                     EEPTLEEIFMHYYK"
     gene            1917..2726
                     /locus_tag="VFH83_02515"
     CDS             1917..2726
                     /locus_tag="VFH83_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6485263.1"
                     /translation="MRATIYRHELRARFRSVIIWSVAVGAIVFLFSSLFPGFASQAAA
                     LKQMFDRFPPALLQAFGMQNTDLSTVLGFYGFVFVFVQLCLAIQAGNYGFGLVSIEEA
                     ELTADFLLSKPVSRARVMTGKLLAAVTSLAITNLVVWAVSFLSLGLFNGGRPYDAGTL
                     VLVLSSGMIFQVFFLGVGLLISLAVRRVRSVTPFSLALGFGMYFLSAFSSVLSEVKLE
                     YITPFKHFDPAAIVRNATLDTRFVLPNVAVSILAVAVSYGLYIRRDIQAVS"
     gene            2738..3532
                     /locus_tag="VFH83_02520"
     CDS             2738..3532
                     /locus_tag="VFH83_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6485264.1"
                     /translation="MNIFLRELRSNLKSLLIWSGIMILLVLVGLAKFAAYYNNPSSLE
                     ILSSLPKGMVDAMSLQAFNLTTLSGLYGILFVFFGLLGGIAAAMWGSTLISKEERDKT
                     VEFTLVLPVSRARIVTAKALAAAVDCVIFVLVTWVMSVLGAQSYKPDRAFQSFLALEM
                     AAMLVVELVFLAVGLLLGCAMKRYKRSGSVAVAILLVAYFLSVVTGLQTNLGFLRYFT
                     PLKYFDPARMLRTGSLEGGFLALSAGIVVVCLAAAYGVYRRRDLYL"
     gene            3698..>4572
                     /locus_tag="VFH83_02525"
     CDS             3698..>4572
                     /locus_tag="VFH83_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HET6485265.1"
                     /translation="MQRLDLAGEWRLARASTGDAVPAAVPGDTHSALMAAGRIPDPYL
                     GRNELDVQWVGREDWVFERAFQIPRDFLSEASVLLNCDRLDTIADVEINGRRAGHADN
                     MFERWRFEVKRLLKPGRNHIRVRFRSAEKEALAESRRLPYPIPHTTNPVQSMHRNLVR
                     KVQCHSGWDWGPCLMVAGIYGDISLQATSQARIEYVYTEQQHSRNRCRLRVFVECEVI
                     TPGEYPLTVDLDGQQGSHTFTLAKGRHRVSVDVSVADPRLWWPNGLGEQKLYGLTVEL
                     GGDRVQKRIGLRSLEL"
BASE COUNT          788 a         1444 c         1431 g          909 t
ORIGIN      
        1 ggctacggta tcgtctccgg aacggcgctg gtcatggaag ccgacggttc ggtgacggcg
       61 cgcggcggag aggtcgacgt gttccgccgc cgcaagaacg gcgtctccca gctcaagagc
      121 ctgactccca tccctgcgcc agactgaccg ttgacagggg gcgccccctg tcaggcttgg
      181 cctggactct tcgaacgagc cgtctgtagg ttgcgcttgg gtgagccttg atacccgcgc
      241 ttgacaaata aaccgtctga cggtatattt ttctttaggc ggcgaggggc agaggtagat
      301 agatggctcg gaccgtcaag ccagcggaac atgaggccaa gcgcaacgag atcctggatg
      361 ctgcggcggg gctggtctat tccaagggat acgagaagat gacgatccag gacctcctgg
      421 acactctgag gatctcccgg ggcgcgcttt accactattt cgtttccaag cagcagttgg
      481 tcgaggcact gatcgagcgt tccgccggcg acatggaaag gtcgctcctt gcgatcgtgc
      541 aggacccggg tctttccgcg gttgagaaga tccagggcta tttcgacatc gccacgcgct
      601 ggaagacatc gcagaaagac ctcatcatgg gcgcgcttcg aagctggtat tccgacgaca
      661 acgtgctcct cagacagaaa ctgggcgaga aatcgcgtcg ctatgtatcg cgtctcctcg
      721 aaccgatcat ccaccagggt gttcaggaag ggctgttcgc taccccctat ccggcggaga
      781 ttgcggcaat cttcgccggc atgacgctga acctctcgga ctgcctggtg gagctcatgc
      841 tcgccgagcc tcccgaaccg gggacgcttc gcagagcgca ggcgctcctg gacgcctaca
      901 cggtctcgat cgaacgcatc ctgggcgccg gcgccggttc gttcagaccc gtgaacttcg
      961 gtgtcttcaa ggattggttt tcgccggaac gcgggtaaac aaaaaagctc accagcacgc
     1021 aaaggagggt caccatgagc gtcatcgaag tggcggatct gaccaagttc tacgggaagg
     1081 cgcgagggat cgtggacgtc tcgttccgcc aggaagaggg ggagatcttc ggcttcatcg
     1141 gcccgaacgg cgccgggaaa tccaccacca tccgcctcct cctctccctt atccacccta
     1201 cgagcggcag cgcgcggatc ttcggcaagg acgtcgtctc ggagggcccg cggatccggc
     1261 gggacatcgg ctaccttccc tcggaagtct tctattacga gggcatgaaa gtgatcgacc
     1321 ttctgaagta ctccgccagt ttttttggcg gcaatcgaac ccggcggatc caggagctct
     1381 ccgacaccat ggagctcgag ctcggacgcc gcatcagcga cctctcgtac gggaatcgga
     1441 agaaggtggg tatcgtgcag ggccttctgc acagcccgaa gcttctgttt ctcgacgagc
     1501 cgacgagcgg gttggatcca ctgatgcagc gcaggttctt tgacctcatc cgaaaggaga
     1561 acgagcgcgg cgtgacggtc ttcttctcct cccacatcct gggcgaggtg cagcggatgt
     1621 gcaaccgcgt gggcatcatc cgcgaaggca ggatcgtgga gatctccgac atccgcgcgc
     1681 tgcagcagag cacctacaag aaggtgaacg tctccgggga tcatctgcag cccgccgcct
     1741 tccgtctgcc cggggtggaa caactggaag aggaggatgg atcgcttcgg ttctttttca
     1801 agggcgacat caatgccctg gtccgtgcca tgagcacggt tcgggtttcc aacgtgacca
     1861 tcgaagagcc cacgctggaa gagatcttca tgcactacta caagtaagga ggaggcatgc
     1921 gcgcaaccat ctatcggcac gagcttcgtg ccaggttccg atccgtcatc atctggtcgg
     1981 tagcggtggg agcgatcgtg ttcctgttct cctcgttgtt ccccgggttt gcctcccagg
     2041 ccgccgccct caagcagatg ttcgaccgct tcccgcccgc gctgctccag gcctttggga
     2101 tgcagaacac ggatctctct accgtgcttg gattctatgg attcgtgttc gtctttgtgc
     2161 agctctgcct ggccatccag gccggcaact acggatttgg tctggtctcg atcgaggagg
     2221 ccgagctcac ggcggacttt ctgctgagca agcccgtgag ccgcgcgcgc gtcatgaccg
     2281 gcaagctgct tgccgcggtc acgagcctcg ccatcacgaa cctcgtcgtc tgggcggtga
     2341 gcttcctttc gctggggctg ttcaacggag gtcggcccta cgacgcgggc acgctggtcc
     2401 tggtgctttc cagcggcatg atcttccagg tgttctttct gggcgtcggc ctgctcatct
     2461 ccctggcggt ccggcgtgtg cgcagcgtga cgcctttctc cctggcgctg gggttcggca
     2521 tgtacttcct gagcgccttc agctccgtcc tttcggaggt caagctggag tacatcacgc
     2581 cgttcaagca cttcgacccg gctgccatcg ttcgaaacgc cacgctggac acgcgcttcg
     2641 tgcttcccaa cgtcgcggtg agcatcctgg cggtggcggt gagctacggg ttgtacatcc
     2701 gtcgagacat ccaagcggtg tcgtagggga ggcaccaatg aacattttcc ttcgcgagct
     2761 ccgatcgaac ctgaaatccc tgctgatctg gagcggaatc atgatcctgc tggtcctggt
     2821 gggcctggcc aagttcgccg catactacaa caacccctcc tcgctggaga tcctgagctc
     2881 ccttcccaag ggaatggtcg acgccatgag cctccaggcg ttcaacctga caacgctgag
     2941 cggcttgtac ggcatcctgt tcgtcttttt cggcctgctg ggtgggattg ccgccgccat
     3001 gtggggcagc accctgatct ccaaagagga gcgggacaag acggtggagt tcaccctggt
     3061 gctccccgtg tcacgcgcca ggatcgtcac ggccaaggcg ttggccgcgg cggtggactg
     3121 cgtgatcttc gtgctggtca cctgggtcat gtccgtgctg ggggcccaat cctacaagcc
     3181 ggaccgcgct ttccagagct tcctggccct ggaaatggcg gcgatgctcg tggtggaact
     3241 ggtcttcctc gccgtcgggc ttctgctggg atgcgccatg aagcgataca agcgttcggg
     3301 ttccgtggcc gtcgccatcc tgttggttgc ctacttcctt tccgttgtca ccggcctgca
     3361 gaccaacctg ggattcctgc ggtacttcac cccgctgaag tatttcgacc cggccaggat
     3421 gctcaggacc ggcagcctgg aaggcggatt cctcgcgctc tctgccggca tcgttgtggt
     3481 gtgcctcgcc gcggcgtacg gggtctaccg aaggcgggat ctctacctct gacctcgcag
     3541 gccgctgacc ctccagaccg cacccctcgg cgacggtgcg cgccgttgca cctggccgcc
     3601 cggataggct atcctcgggg aaacgaaacc tcggcgtccc ccggccggtc gccttcgcgc
     3661 gcgccgacgg acggtgtcca tctcgaggag gagcctgatg caacgcctag acctggccgg
     3721 tgagtggcgc ctggcgcgcg cctcgacggg agacgccgtt cctgccgcgg tgccgggaga
     3781 cacccattcc gcgctcatgg ccgccgggcg gatcccggat ccgtacctgg gcagaaacga
     3841 gctggacgtg cagtgggtgg gtcgcgagga ctgggtcttc gagcgcgcct tccagatccc
     3901 ccgggatttc ctgtccgaag cgtcggtcct cctgaactgt gatcgcctgg ataccatcgc
     3961 cgatgtcgag atcaacggtc ggcgcgccgg ccacgccgac aacatgttcg agcggtggcg
     4021 gttcgaggtg aaacggctct tgaagcccgg ccgcaaccac atccgggtcc ggttccgctc
     4081 cgcggagaag gaagcgctcg ccgagtcgcg ccgtctcccg taccccatcc cgcacaccac
     4141 caacccggtg cagtccatgc accgcaacct ggtccgcaag gtccagtgcc actccggttg
     4201 ggactgggga ccttgtctca tggtggcggg gatctacgga gacatctccc tccaggcaac
     4261 ctcgcaggcc cgcatcgaat acgtgtacac ggagcagcag cactcccgaa accggtgccg
     4321 tctgcgcgtg ttcgtggaat gcgaggtgat caccccgggc gagtacccgc tcaccgtgga
     4381 tctggacggt cagcagggat cccatacgtt cacccttgcc aagggacgcc accgggtgag
     4441 cgtcgatgtc tcggtagccg accctcggct ctggtggccc aacggcctgg gagagcagaa
     4501 gctttacggt ctcacggtgg agctgggcgg ggaccgggtc cagaagagga tcgggcttcg
     4561 ctccctggag ct
//
LOCUS       DASRZS010000105         3634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3906793, whole genome shotgun sequence.
ACCESSION   DASRZS010000105 DASRZS010000000
VERSION     DASRZS010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3906793"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1909)
                     /locus_tag="VFH83_02530"
     CDS             complement(<1..1909)
                     /locus_tag="VFH83_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6485266.1"
                     /translation="MNITELSVRRPSAIIVGIIFIVGLGVLGYVNLGADLFPSVNTPI
                     ISIRSTYMGAGAEQIDQDVVKPIEDAVSGVNGIDIIRSTSGEGYGYTILQFHLDTDIN
                     AAVIDVQKAIDGIAATLPAEATRPVMQKFDINSQPILILSVSGSLEPDELYNQADRIR
                     LAVESMPGVGAVSLQGGQKRQLRIVLDRTALQYYSVSTNALLGTLKADNLSVPEGEID
                     QPGRQLTVRLTGELADLEDVRNLRIPTLVGSSVRLADLGQISLDYPDATETVRMDGSS
                     SIGIFVRKQSDANVVTTADGVKQALAGLQSTLPPGVTVKVASDATLFVNSSLAETRLN
                     LLESILTTSLVLFLFLRQWRSSLIVLIAIPISLIGTFFAMYALHYTLNIVSLMALGLC
                     IGILVDDSIVILENVDRHIHLGQDPMRAAVDGRKEIGLAAVAITLCDVVVFAPIAFLQ
                     DIVGQFFKEFGLTVVCATLLSLLVSFTVTPMMASRMLAGRRGKRSQRIEPPEGSAEER
                     TKGFGGFFDRTIKGSYRQVLLWALRNRWKVVFGIGILVVASLALLPLGVIQSEFMPGF
                     DQSKLVVDLDLGAGAGYPQTDQKVRIVEKYLLGLPEVTDTFATIGSDLSSSVAEIVVK
                     LKDKGQRRKSQT"
     gene            complement(1920..3341)
                     /locus_tag="VFH83_02535"
     CDS             complement(1920..3341)
                     /locus_tag="VFH83_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6485267.1"
                     /translation="MRTSSAAAVGFLSLLLTCPAAWPDALTLDQAYDLARGASEAIHV
                     KALAVRKAGSAVQEAQARALPHLDLQASTSYLTNPPTGITVTAGEFGTFSPEIPPHTL
                     SPLQSSPISLGTVSLPSSDVQVGAQLHNYFSLAASLSQPLFTWGKIKNAIDLAGIQAQ
                     AAATDLTAQQRDIARQVHRSYFSAQLALQSIPVLVHLRDLAAGAAKDRQTSFDQGTVN
                     RQSLLEAQANLAQVESKLVQAQQSAATALEGLSVLTGADPSATTLSTDFRTGMPAIDE
                     QALLGKAIASSTDLARARSTAGQAGKALDIARGGSLLLPDVSLGVNFNVAGQEDLPYS
                     AWDWNNNTWSWDVVISLGVKLSVFDGLAAARKVEEAQRDVEMAAQGTSQQEKLVRLSV
                     RQAVDAALRADADASDKRAAADLGAERLKNAQASFDNGMASLDDLRGADILYGSARLD
                     LLLALYTREEALADLERITGERL"
     gene            complement(3344..3616)
                     /locus_tag="VFH83_02540"
     CDS             complement(3344..3616)
                     /locus_tag="VFH83_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485268.1"
                     /translation="MRLLQENLERFCALVERLLPPATPRSARGAPRTGGASLSARVSG
                     SVARRRALIRLTVVGVLFTAALADPDCVDSEIDGLKRSVMAMLSGG"
BASE COUNT          615 a         1194 c         1224 g          601 t
ORIGIN      
        1 ccgtctggct cttcctccgc tggcccttgt ccttgagctt caccacgatc tccgccacgg
       61 agctcgagag gtcgctcccg atggtggcaa aggtgtccgt gacctccggc aggcccagca
      121 ggtacttctc cacgatcctt accttctggt ccgtctgcgg gtaaccggcg cccgcgccaa
      181 gatccaggtc caccaccagt ttgctctggt cgaagcccgg catgaactcg ctctggatca
      241 cgcccagcgg caggagcgca aggctggcca ccaccaggat gccgatcccg aacaccacct
      301 tccaccggtt gcgcaaggcc cagaggagca cctgccggta gctgcccttg atggtccgat
      361 cgaaaaagcc gccgaacccc ttggtgcgct cttccgcgga gccttccggg ggttcgatcc
      421 gctgggaacg tttccctctt cggcccgcga gcatccggga cgccatcatc ggcgtgaccg
      481 tgaaggaaac gaggagggag aggagggtgg cgcacacgac ggtgagcccg aactccttga
      541 agaactgacc cacgatatcc tggaggaacg cgatgggcgc gaagaccacc acgtcgcaga
      601 gcgtgatggc cacggccgcc aggccgatct cctttcgacc gtccaccgcc gcgcgcatgg
      661 ggtcctgacc caggtgaatg tgacggtcca cgttctccag gatcacgatg gagtcatcga
      721 ccaggatccc gatgcacaac cccaacgcca tgagggagac gatgttcagg gtgtagtgca
      781 gcgcgtacat cgcgaagaac gtcccgatca gggagatggg gatggcgatc agcacgatca
      841 gcgacgaccg ccactgccgg agaaacagga acaggaccag cgacgtggtc aggatgcttt
      901 ccaggaggtt gagccgggtc tccgcaaggg aggagttgac gaacagggtg gcgtcactgg
      961 ccaccttcac cgtcaccccc gggggcaagg tggactgcag acccgccagc gcctgcttca
     1021 ccccgtccgc cgtggtcacc acgttggcat cgctctgctt gcggacgaag atcccgatgg
     1081 aagaagagcc gtccatgcgg accgtctccg tcgcgtcggg atagtccagg gagatctggc
     1141 ccaggtcagc cagtcggacg ctgcttccca ccagcgtggg gatgcgcaga ttgcggacgt
     1201 cctcgaggtc cgcaagctct ccggtgaggc ggaccgtgag ctgccggccc ggctggtcga
     1261 tctctccttc gggcacggaa aggttgtccg ccttgagggt ccccaggagg gcattggtgc
     1321 tcaccgagta gtactgcagg gccgtgcggt cgaggacgat gcgtagctgc cgcttctggc
     1381 cgccctggag ggaaaccgcg cctacgccgg gcatgctctc gacggcaagc cggatccggt
     1441 ccgcctggtt gtacagctcg tccggctcca ggcttcccga gaccgagagg atcaggatgg
     1501 gctggctgtt gatgtcgaac ttctgcatga ccggccgcgt ggcttccgcc ggcagcgtgg
     1561 ccgcgatgcc gtcgatggcc ttctgcacgt cgatcaccgc ggcgttgatg tccgtatcca
     1621 ggtggaactg gaggatggtg tagccgtatc cctccccgga ggtggaccgg atgatgtcga
     1681 tcccgttgac gcccgagacc gcgtcctcga tgggtttgac gacgtcctgg tcgatctgct
     1741 ccgcgccggc tcccatgtag gtcgatcgga tggagatgat gggcgtgttc accgacggga
     1801 acagatccgc gcccaggttg acgtacccca gcacgcccag gccgacgatg aagatgattc
     1861 cgacgatgat ggccgagggt cggcgtaccg aaagctccgt gatgttcatt ccccagtcct
     1921 cacaaccgct ccccggtaat gcgctcgaga tcggccagcg cctcctcccg ggtgtacaag
     1981 gccaggagca ggtccagccg cgcggacccg tagaggatgt ccgcgccgcg tagatcgtcc
     2041 aggctcgcca tcccgttgtc aaaggaggct tgagcgtttt tcagccgctc cgcgcccagg
     2101 tccgcggcgg ccctcttgtc cgaggcatcc gcgtccgcgc gaagggccgc gtccaccgcc
     2161 tggcggaccg agagccttac gagcttctcc tgctggctcg tcccctgggc cgccatctcc
     2221 acgtcccgct gcgcctcttc gaccttgcgc gccgcggcca gcccgtcgaa cacgctgagc
     2281 ttcacgccga ggctgatcac gacgtcccag ctccacgtat tgttgttcca gtcccaggcg
     2341 gaatagggga gatcctcctg gccggccaca ttgaagttca ccccgagaga aacgtccggt
     2401 aggagcagcg accctcctcg agcgatgtcc agggccttgc ctgcctggcc cgccgtggag
     2461 cgcgccctgg ccagatccgt ggaagaggca atggcctttc ccaagagcgc ctgctcgtca
     2521 atcgccggca tgcccgtccg gaagtccgtg ctcagcgtgg ttgccgatgg gtccgcgccg
     2581 gtcaacacgc tcagcccctc caacgccgta gcggcgctct gctgcgcctg gaccagcttg
     2641 ctctccacct gggccaggtt ggcctgggct tccaggagag actggcgatt caccgtgccc
     2701 tggtcgaagg aagtctggcg gtccttcgct gctccggcgg ccaggtcccg caggtgaacc
     2761 aggaccggga tcgactggag cgcgagctgg gcggagaagt aggagcgatg gacctggcgg
     2821 gcgatgtccc gctgctgagc cgtgagatcc gtggccgccg cctgggcctg gatgccggca
     2881 aggtcgatgg cgttcttgat ctttccccac gtgaagagcg gttgggaaag cgacgccgcg
     2941 agggagaagt agttgtgaag ctgcgcgccc acctggacgt cggaggaggg gaggctgacg
     3001 gtgcccaggg agatgggaga gctttgcagg ggcgacagcg tgtgcggcgg gatctccggg
     3061 ctgaacgtcc cgaactctcc cgccgtgacg gtgatccccg tcggcgggtt ggtgaggtac
     3121 gaggtggagg cctggaggtc caggtgcgga agcgcccggg cctgggcctc ctggaccgcg
     3181 gacccggcct tgcgcaccgc gagcgccttc acgtggattg cctccgatgc gccgcgggcc
     3241 aggtcataag cctggtccag ggtgagcgcg tcaggccagg ccgccgggca ggtgaggaga
     3301 agggagagga aaccgacggc agccgcggat gacgtacgca tgctcatccc cccgagagca
     3361 tcgccatcac ggatcgcttc agtccgtcga tctcggagtc gacacagtcg gggtccgcaa
     3421 gcgccgcggt gaagagcacc ccaaccacgg taagccggat gagcgccctc cgccgcgcca
     3481 cggagccgct cacccgggcc gagagggaag ccccaccggt ccggggggcg ccgcgcgcgg
     3541 atcgcggagt ggcgggaggc aggagccgct ccaccagcgc gcagaagcgc tcgaggtttt
     3601 cctgcagcag cctcatctgg ctggactaga tcgg
//
LOCUS       DASRZS010000106         3185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5447927, whole genome shotgun sequence.
ACCESSION   DASRZS010000106 DASRZS010000000
VERSION     DASRZS010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5447927"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..208)
                     /locus_tag="VFH83_02545"
                     /pseudo
     CDS             complement(1..208)
                     /locus_tag="VFH83_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            323..1042
                     /locus_tag="VFH83_02550"
     CDS             323..1042
                     /locus_tag="VFH83_02550"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6485269.1"
                     /translation="MPAPAIPKYYAISQEIINRIRAGDLEPGSRIPSENEIIRRYRVS
                     NTTARKALLEIEKTGWGLKIKGKGTIVRSQRVVRSVTRILSFTNNMVEAGYRPSTRVL
                     ERRILRGGYAATINGRRYAIRGPVCCIHRLRFADEQPMLYEIRYINLGLCPTIARLDL
                     GSSLYRLYEREYGLELSEIHQMLSAVMIETGIREHFGLEEPIPGFLVEGVTFCGKELI
                     LEMERSVYRGDKYRFAVRALS"
     gene            1104..2087
                     /locus_tag="VFH83_02555"
     CDS             1104..2087
                     /locus_tag="VFH83_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6485270.1"
                     /translation="MRELKVSVGMWYMGATGDRFVKAGYRADRTIEERFLAATKIPGV
                     GGLEMHYPTEINDQNLPKLKRIADDHGLRFVMLTPHLWIDPVYRFGQFSNPDPQLRRR
                     AIDFAKRVTDVGARLNVEFLCYWPAQDGYDYHFQVDYMRRWDTLAEGLAEWADYNPKQ
                     RIVVEYKAYDPRAHILLPNVGQTVTLLNEINRPNLGINIETGHALIMKETLAETFAFA
                     MRYKRLFHTHWNDNSKMYDDDAMVGMVNLWETLELLFWLEELAYDGWFGLDLFPFREM
                     PEDAVAQSIANLRMGYEMLDRVDRKELKACFESSDAIEISRLMRKMLGGKG"
     gene            complement(2106..2249)
                     /locus_tag="VFH83_02560"
     CDS             complement(2106..2249)
                     /locus_tag="VFH83_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485271.1"
                     /translation="MGDLCGNGRADVLVAEIGVADYKTRAYLGRPPHGPEMWNIHVYE
                     RAR"
     gene            complement(2446..>3185)
                     /locus_tag="VFH83_02565"
     CDS             complement(2446..>3185)
                     /locus_tag="VFH83_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485272.1"
                     /translation="CEALAPHLLHSALAGADPSGQVPVTLWMHRGCVPFVSPRDFRDI
                     YWATLRPILQEIWAQGHQVLLYAEGTWAAHLESFAELPDHSVIFHVDRTDLERVRRVL
                     GGRFCLSGGIPNDLLTTGTPAQVRARCRQVIETLGADGGYILDASALIMDDARVENVE
                     AVVEAALEFGVYSRAASPAAWPPGPPPTPSQPTVARTRMPPGVCVPWEEKRLEFPEIT
                     GDESVARRAWEAVDGMGYGFLWTNLTW"
BASE COUNT          577 a         1029 c         1052 g          527 t
ORIGIN      
        1 cgagggccag gaccagcacc ccgctcgcct ggatcatgcg gcggttgaat cgcgcactga
       61 ggagggagct caccgctccc agccccagga ggagtgggac cgtcccgacg ctgaagagaa
      121 acattgaaag cgctccggct aggaggctgc cggtgcccag cgcgtagacc tgcatggtct
      181 gcagagggcc gcagggcatg agcccggatt ggcagcttct attctgccgc aaggatgtga
      241 agatatcttg aaggaggacg atctacgggt gggatccgga gcgttcgttg acaggctccg
      301 gcgcggttcc tatcctcagc ccatgcccgc accagccatc ccgaagtact atgcgataag
      361 ccaggagatc atcaacagga tccgcgccgg ggacctggag ccggggtccc gcatcccatc
      421 ggagaacgag atcatccgtc ggtaccgggt cagcaacacg accgcgcgaa aggcgcttct
      481 ggagatcgag aagaccgggt gggggctgaa gatcaaggga aagggcacca ttgtccgcag
      541 ccagcgggtg gtgcgatccg taacccgcat cctcagcttc accaacaaca tggtggaggc
      601 cggctaccga ccatccacgc gggtcctgga gcgcaggatc ctcaggggag gctacgcggc
      661 caccatcaac ggacgccgtt acgccatccg cgggcccgtt tgctgcatcc accggctgcg
      721 attcgccgac gagcagccca tgctgtacga gatccgctac atcaacctcg gtctctgccc
      781 gacgatcgcc cggctggacc tcggctcctc gctgtatcgc ctctacgagc gggagtacgg
      841 tctggagctc agcgagatcc accagatgct gagcgccgtg atgatcgaga ccggcatccg
      901 ggagcacttc gggctggaag agcccatccc cggcttcctg gtggaagggg tgacgttctg
      961 cggcaaggag ctgatcctgg aaatggagcg gtcggtctac cggggagaca agtaccgctt
     1021 cgcggtccgc gcgctgtcct gacgcctccc gcgcggttac cttctccctg tgccgccgca
     1081 gaggcggagg aaggggtcac cgcgtgaggg agctgaaggt atccgttggc atgtggtaca
     1141 tgggcgcgac cggggatcgg ttcgtgaagg ccggctaccg ggcggaccgc acgatcgagg
     1201 agcgtttcct ggctgccacg aagatccctg gggtgggagg tctggagatg cactatccca
     1261 ccgagatcaa cgaccagaac ctcccgaagc tgaagaggat cgcggacgat catggccttc
     1321 ggttcgtcat gctgacgccc cacctttgga ttgacccggt ctatcggttt ggccagttca
     1381 gcaacccgga cccgcagctt cgacggcggg ccatcgactt tgccaagcgg gtcacggacg
     1441 tgggggcccg actcaacgtg gagttcctgt gctactggcc ggcgcaggac gggtacgact
     1501 accacttcca ggtggactac atgcgccgct gggacacgct ggccgaaggg ctggccgagt
     1561 gggcggacta caacccgaag cagaggatcg tggtggagta caaggcgtac gaccctcgcg
     1621 cccacatcct cctgcccaac gtgggacaga ccgtcacgct gctaaacgag atcaaccggc
     1681 ccaacctcgg catcaacatc gagaccggcc acgccctcat catgaaggag accctggcgg
     1741 agacctttgc cttcgccatg cgctacaaac ggctcttcca cacccactgg aacgacaact
     1801 ccaagatgta cgatgacgac gccatggtgg gcatggtcaa cctgtgggag accctggagc
     1861 tgctcttctg gctcgaggag ctcgcctacg acggctggtt cgggctggat ctgttcccgt
     1921 tccgcgagat gcccgaggat gccgttgccc agagcatcgc caatctccgc atgggctacg
     1981 agatgctgga tcgcgtggac cgcaaggagc tcaaggcttg cttcgagagc tcggacgcca
     2041 tcgagatctc ccggttgatg cggaagatgc tggggggcaa gggctgagga gcgcggcgcg
     2101 gcgcttcagc gcgcacgctc gtagacgtgg atgttccaca tctcggggcc gtgcggcggt
     2161 ctgccaagat aggcgcgggt cttgtagtcc gccacgccga tctctgccac cagcacgtcg
     2221 gccctgccgt ttccgcacag gtcgcccacc cgtagggagt gcgcgtccag gaggccgtcc
     2281 tccagcaggt gctcctccca gggtccggcc gggtcaccct ttggcttgaa ccaccctgcc
     2341 cggcctctct gcggtcttcc gtagatgcag ggatcaccct cgccctcttg acgtcgacag
     2401 ctttcatcgc ggccccttcc gtttcaccag ggtgctgatg cgtccctacc aggtcaggtt
     2461 ggtccagagg aaaccgtagc ccatgccgtc caccgcctcc caggctcgac gcgccacact
     2521 ttcgtcccca gtgatctcag ggaactcgag gcgcttctcc tcccagggaa cgcagacgcc
     2581 gggcggcatc cgagtccgcg ccacggtggg ctgcgacggg gtgggcggcg gcccgggcgg
     2641 ccatgccgcc ggagaggcag cccggctgta gaccccgaac tccagcgcag cctcgaccac
     2701 tgcctcgacg ttctcgaccc gggcatcgtc catgatgaga gcgctggcgt ccaggatgta
     2761 cccaccgtcc gcgccgaggg tctcgatcac ctgtctgcac cgcgcgcgga cctgcgcggg
     2821 tgtgccggtc gtgagaaggt cgttcgggat tccgccgctg aggcagaagc gtccgcccaa
     2881 cacccgtcga acacgctcga ggtcggtgcg gtccacgtgg aagatcacgc tgtggtcggg
     2941 aagctcggcg aagctttcca ggtgggcggc ccaggtgcct tccgcgtata ggagcacctg
     3001 gtgcccctgg gcccagatct cctggaggat gggtctgagg gttgcccagt agatgtcgcg
     3061 aaagtccctc ggcgagacga acggaacgca gccgcggtgc atccagaggg tgacggggac
     3121 ctggcccgag ggatcggccc ccgccaacgc ggagtgaaga aggtgaggcg ccagggcctc
     3181 gcagg
//
LOCUS       DASRZS010000107         4906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3521200, whole genome shotgun sequence.
ACCESSION   DASRZS010000107 DASRZS010000000
VERSION     DASRZS010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3521200"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="VFH83_02570"
     CDS             complement(<1..1616)
                     /locus_tag="VFH83_02570"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HET6485273.1"
                     /translation="MSDQKLSLQTATSTLGFDRDSGRLISFTSKAAPDQQFVASAPDH
                     PVFLLHYVDADGQYQPLDSFSAREHAVTLEGGETSGGLSILYRGVGGMDLDVLGTVTV
                     SPDDPFARWRVSVTNRAGLRIVDVQYPFVVCAYELGGSPGAETIVLPHDFGNLIRAPT
                     FEKLGPDSLYAWQFTWRNGSFNHYPGGWFAQFLAYYNDRAGIFLATQDTEACVKRFHC
                     LHRHPGFRMGVAHVGDWPSSGERTLEYDTVLGSFAGDWYDAADLYRSWSLKQKWAVPL
                     AKRSDVPAWLLGSPVHVTIRPQGILDAGPVLPIDEFLPYEKCVPLLQGIADRVAAPLV
                     AVIMGWERAGSWVYPDCFPPVGGEDSVTRFARMCRQRGWHVGSFCNGTRWVVGHSWNG
                     YDGRPYYQRHNGEASISRESDGRGWVETWDAGWRPSIVQCMGAEPTRRIAREFVERLL
                     AWGLESIQFFDQNCGAATFPCFSDSHGHPSVPGKWMARAMEETVEEFRRAAERAGEPE
                     AIQSVEMCCNEHNLQLFQQSDSRVRPPGHVV"
     gene            complement(1680..3137)
                     /locus_tag="VFH83_02575"
     CDS             complement(1680..3137)
                     /locus_tag="VFH83_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET6485274.1"
                     /translation="MTGGLRIHPSNPKVLEYRGVPRMLVCATEHYGAVINRPFRFERY
                     LQAAAEGSQTLTRLFLLFRELQSDRNPYSTAKPESPDFVAPFVRTGPGRALDGEPQYD
                     LSRWNPEYFDRLHRFLALARDLGVVVEVTLFSNTYAPQIWALNPLNARNNVNGTEEID
                     WPDYLTLRHPVLVRWQHAYLRKVVEETRQYDNILYEVCNEPGGKAPGRATNPSCDEVN
                     AWQREIVRIIRETEAGQRHLISGQEAFTYEPFTQASDLSFTDMPFDIVNVHPLPGTRY
                     RGETYGMGEFMSKQLTLRAVQGFCQATASEPRPLNLDEDNVASQYKDQDGWTIHRKRA
                     WVSLLSLRHYDYIDFSILPLREEGTEESRAAIRAWMKHLSRFIHSVDLPRAACRPGFL
                     SQAPQHVLASVLAVEGRDYCIYLADERELSEPGAGGEIQGAVSFELPAGVWRAACFSP
                     TTGLYSPWIEIGRGGKTEFRVPSFQQDIVIRIRTA"
     gene            3524..3880
                     /gene="trxA"
                     /locus_tag="VFH83_02580"
     CDS             3524..3880
                     /gene="trxA"
                     /locus_tag="VFH83_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET6485275.1"
                     /translation="MTESLTKETFTQKVFDFEKNQEWKFAGSVPAIVDFWATWCGPCR
                     MISPVLEEISKEFDGKLNVYKVNVDEQQELAAVFGVQSIPTLLFIPLNDSPRLAAGAM
                     PKAGLLKLVKDVLKVE"
     gene            complement(3894..4844)
                     /locus_tag="VFH83_02585"
     CDS             complement(3894..4844)
                     /locus_tag="VFH83_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6485276.1"
                     /translation="MGDLIQGIHHVTALAGDPQKCLDFYGGILGLRLVKKTVNFDAPD
                     VYHLYLGDEAGRPGTILTFFPYGTILQGRPGNGMAVCVAFSVPTRARAFWEERLTRFG
                     VRFERVPDGFPSETAIQFSDGDGLALRLSFTETDPRVGYGTGSITSQNAIRGFHAVEL
                     WEESLEPTRALLCDYLGHRLVARSGDTSRFSSSETPGCYVDILVRPKGARGRGGKGTV
                     HHVAFATEDRGTQTTLRGALGGIANPTPIIDRQYFTSVYFHEPGGVLFEVATLGPGFA
                     VDEPLEALGGALKLPPQYEPARGRIESELTPIALDADRYR"
BASE COUNT          831 a         1637 c         1660 g          778 t
ORIGIN      
        1 acgacatgac cggggggacg gacccgggag tccgactgct ggaagagctg gaggttgtgc
       61 tcgttgcagc acatttccac ggactggatg gcctcgggct cccccgcgcg ctctgcggcg
      121 cggcggaact cctccacggt ctcctccatc gcccgagcca tccacttgcc gggcacggag
      181 gggtggccgt ggctgtccga gaaacagggg aaggttgccg cgccgcagtt ctgatcgaag
      241 aactggatgg actccaggcc ccaggcaagg aggcgctcca cgaactcccg ggcgatccgc
      301 cgggtgggct cggcgcccat gcactggacg atgctggggc gccagccagc gtcccacgtc
      361 tcgacccaac cgcggccgtc cgactcgcgg ctgatgctgg cctcaccgtt gtgacgctgg
      421 tagtagggac ggccgtcgta gccgttccag ctatgcccca ccacccaacg ggtcccgttg
      481 cagaaggagc ccacgtgcca gccccgctgc cgacacatcc gggcgaaccg cgtcacggag
      541 tcctctccgc cgacgggcgg gaagcaatcc gggtacaccc aggaacccgc gcgctcccat
      601 cccatgatca ccgccaccag cggtgccgcg acgcggtcgg cgatcccctg gagcaggggc
      661 acgcacttct cgtacggcag gaactcgtcg atgggaagga ccggtccggc atccaggatt
      721 ccctggggcc ggatggtgac gtggaccgga gagcccagga gccaggcggg gacgtcggac
      781 ctcttggcca gcggtacggc ccacttctgc ttcaggctcc agctccgata gaggtccgcc
      841 gcgtcgtacc agtctcccgc aaaggacccc agcaccgtgt cgtactccag cgtacgctcc
      901 ccggaagagg gccagtctcc cacgtgagcc acccccatgc ggaagccggg gtgccggtgg
      961 aggcaatgaa aacgcttgac gcaggcctcc gtatcctggg tggccaggaa gatgccggcg
     1021 cggtcgttgt agtacgccag gaactgcgcg aaccatccgc cagggtagtg gttgaagctg
     1081 ccgttcctcc aggtgaactg ccaggcgtag agggaatccg gccccagctt ctcgaacgtg
     1141 ggggcccgga tcaggttccc gaagtcgtgc ggaaggacga tggtctcggc gcccggcgag
     1201 ccgcccagct cataggcgca taccacgaac gggtactgca cgtcgacgat ccgcagcccg
     1261 gcgcggttgg tcacggaaac gcgccatcga gcgaacgggt cgtcgggcga gaccgtgacc
     1321 gtgcccagga cgtcgaggtc catcccgccg acgccgcggt agaggatgct cagtccgccc
     1381 gacgtctcgc cgccctccag ggtgacggcg tgctcacgag cggagaagga gtccaggggc
     1441 tggtactgcc cgtccgcgtc cacgtagtgc aggaggaaca cggggtggtc cggcgcggaa
     1501 gccacgaact gctggtccgg ggcagccttg ctcgtgaagg agatgaggcg gccggagtcc
     1561 cggtcgaagc ccagggtgct cgtggcggtc tggagactca gcttctggtc tgacatggtc
     1621 gcagtcctcc gtcgatggtg attcgaccga tcgcttgtcc cgcgatcgcc tcatccatgc
     1681 tacgcggtcc ggatgcggat cacgatgtcc tgctggaacg atggaacgcg gaactccgtc
     1741 ttgcccccac ggccgatctc gatccagggc gagtatagcc ccgtggtcgg agagaagcac
     1801 gccgcccgcc agacgcctgc cggcagctcg aaggagacgg cgccctggat ctctccgccc
     1861 gctcccggct cggagagctc ccgctcgtcg gccaggtaga tgcaatagtc ccggccctct
     1921 accgcgagca cggaggccag tacatgttgg ggagcctggg acagaaaacc cggccggcaa
     1981 gccgcgcggg gcaggtccac ggagtggatg aaccgggaca ggtgcttcat ccacgcccga
     2041 atggcggcgc gggattcctc cgtcccctcc tcccgcagag gcaggatgga aaagtcaatg
     2101 tagtcgtagt gccgcaggga gagcagggac acccatgccc gcttgcgatg gatggtccat
     2161 ccgtcctggt ccttgtactg gctggccacg ttgtcctcgt ccaggttgag gggccgcggt
     2221 tccgacgccg tcgcctggca gaacccctgg acggcgcgca gggtgagctg cttcgacatg
     2281 aactccccca tcccataggt ctcgccccgg tatcgggtcc ccggcagggg atggacgttg
     2341 acgatgtcga agggcatgtc cgtgaaggag agatccgagg cctgcgtgaa gggctcgtag
     2401 gtgaacgcct cctggcccga gatgaggtgc cgctgtccgg cctccgtttc ccggatgatc
     2461 cggacgatct cccgctgcca ggcgttgacc tcgtcacagg acggattggt ggcgcgcccc
     2521 ggcgccttgc ctcccggctc gttgcacacc tcgtagagga tgttgtcgta ctgccgcgtc
     2581 tcctccacca ccttgcggag gtaggcgtgc tgccaccgga ccaggacggg atggcgcaag
     2641 gtgaggtagt ccggccagtc gatctcctcg gtgccgttga cgttgtttcg ggcattcagc
     2701 ggattgagcg cccagatctg cggcgcgtac gtgttgctga acagcgtgac ctccaccacc
     2761 acccccaggt cccgggcgag cgcgaggaat cggtggaggc gatcgaagta ctccgggttc
     2821 caccgactca ggtcgtattg cggctctcca tcgagcgcgc gtccggggcc ggtccgcacg
     2881 aagggggcca cgaagtccgg ggactcgggc ttggcggtgg agtacgggtt tcgatcggac
     2941 tgcagctccc gaaagaggag gaagaggcgc gtcagcgttt gagagccctc cgccgcggct
     3001 tgcaggtacc gctcgaagcg gaacggacgg ttgatcaccg ctccgtaatg ctcggtggcg
     3061 cacaccagca tgcgcgggac gccgcggtac tcgagaacct tcggattcga cggatgaatg
     3121 cgcaggccac cggtcacttc ggcctccttg cgccgctcca acgcggcggg taagccgcgg
     3181 gccggcggca cggcgccgcg caggatgggt gtgggctccg cccacagccg aggcgcggca
     3241 ttcaggcgcc ccatcggcgc gcgggatgct gccgatgcgg ccttccacca cctgaaccgg
     3301 attccgcaac cccggtaccc cgccggagcg cggttgtcaa ggccgcttcc cggcaagccc
     3361 ggcgggtccc tcgcgcgagc cgtgccgccc cgcggctacg acacgggcgc gggggggcgc
     3421 gcgccccgct tgacgcgcgg cgcgagacgc gatacattgc tatatatagt tttcactata
     3481 tatcgctgcg ccgcaaccaa cgcatgcggg acaggagtgc gccatgacgg agagcctgac
     3541 caaggagacc ttcacgcaga aggtgttcga cttcgagaag aaccaggaat ggaagttcgc
     3601 gggctcggtc cccgccatcg tcgacttctg ggccacctgg tgcggcccgt gccggatgat
     3661 ctctcccgtg ctggaggaga tctccaagga gttcgacggc aagctcaacg tgtacaaggt
     3721 gaacgtggac gagcagcagg agctggccgc cgtcttcggc gtccagagca tccctacgct
     3781 gctcttcatt ccgctgaacg acagcccccg tctggcggca ggcgccatgc ccaaggccgg
     3841 gctcctcaag ctcgtcaagg acgtgctcaa ggtcgagtag aggcgcgggc ggcctatcgg
     3901 tagcggtccg cgtccagggc gatcggcgtc agctccgact cgatcctgcc ccgcgcgggc
     3961 tcgtactggg gcggcagctt cagcgctccc cccagcgctt ccaggggttc gtccacggca
     4021 aagcccggcc ccagcgtggc cacctcgaag agaacgccgc ctggctcgtg gaagtagacg
     4081 gacgtgaagt actgccggtc gatgatgggc gtggggttgg cgatgccgcc gagggcaccg
     4141 cgaagcgtgg tctgggtccc gcggtcttcc gtggcaaagg ccacgtggtg aacggttccc
     4201 ttgccgcccc gtcctctcgc tcccttcggc cggaccagaa tgtcgacata gcagcccggg
     4261 gtttccgatg aagagaatcg actggtgtct ccgctgcggg ccaccagccg gtgacccagg
     4321 tagtcgcaga gcagcgcccg ggtgggctcc agggattcct cccagagctc taccgcgtgg
     4381 aagccccgga tggcgttctg ggaggtgatc gatcccgtgc cgtatccgac tcggggatcc
     4441 gtctccgtga acgacagccg aagggcaagt ccgtccccat cggagaactg gatggcggtc
     4501 tcggagggga acccatccgg cacgcgctcg aaccggacac cgaagcgcgt cagacgctcc
     4561 tcccagaacg cccgcgcccg ggtcgggacg gagaacgcca cgcagaccgc catgccgttg
     4621 ccggggcgcc cctggaggat ggtgccgtac ggaaagaacg tcaggatggt tcccggccga
     4681 cccgcctcgt ccccaaggta caggtggtag acgtccgggg cgtcgaagtt caccgtcttc
     4741 ttgacgagcc tcagacccag gatgcctcca tagaaatcca ggcacttctg cgggtcaccc
     4801 gccagagccg tcacatggtg aatcccttgg atcaggtccc ccatcgcttt cctttctcca
     4861 cgccgtcaac gactacaaag atcgcgcgga acgcggctac cggcga
//
LOCUS       DASRZS010000108         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_831172, whole genome shotgun sequence.
ACCESSION   DASRZS010000108 DASRZS010000000
VERSION     DASRZS010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831172"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1635)
                     /locus_tag="VFH83_02590"
     CDS             complement(127..1635)
                     /locus_tag="VFH83_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HET6485277.1"
                     /translation="MGEEKASGKKNGGKSGSDSPANGSSPASADQPEPAHPAASGEQP
                     KGSGATEAPSLPAPAPLPVTPPAAQLPAVREPRPQRFLFVSKYGLIHDLAWEVKKEGH
                     DVRYHIMQKSERDVGDGFVEKIERWEDCKEWADLIVFDDCEFGTLADRLRAEGKRVVG
                     GTPYTDRLEMDRDFGQEEMRSAGLTTLPRWEFGSFDAAIAFIKASPGRYVIKPSGRAQ
                     NEKVLSFVGQEEDGLDVLTMLEHYRNGWGSKIKSFQLQKYASGVEVAVGAFFNGTDFV
                     QPAFINFEHKRMFNDDIGPQTGEMGTSGFWMGSSTLFQQTLFRMKDRLAAARYCGYVD
                     INCIANSRGIYPLEFTCRFGYPTINLQMEGMLSRWSELLTGMGRGQPVTLRTKKGFQI
                     CVVIAVPPFPFVDAETFRKFSEDAAVIFRKPVNGGVHPCDIRLVDGDWRLAGSSGYAL
                     VVTGSGSTMDDARREAYNRVKNIVIPNMFYRTDIGERWHRDGDLLQTWGYLL"
     gene            1701..>2348
                     /locus_tag="VFH83_02595"
     CDS             1701..>2348
                     /locus_tag="VFH83_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET6485278.1"
                     /translation="MPVAAIRRYQSERVVENREIAPGLFLLSYPRRHDFVPGQTVALT
                     VDRSFPVRFYSIASGNSEELIEVLYDLVPEGELTPKLAGLRPGDPILASTPFGAFCDW
                     DGPSWWVAGGTGVAPFASMVRSGLVKEKRLVHGSRKVESLYLRRYFQELLGDCYVPCC
                     SREEAEGVYPGRPLDWLTRNYLPTRARYLVCGGSLMVVAVRDSLIEAGIPFGNVVA"
BASE COUNT          370 a          759 c          766 g          453 t
ORIGIN      
        1 cggcgggctc gtcctgacgg gcgttgcagc cgtattcctg ttcagcctgg actggtccaa
       61 ggtgtggccg ctcttcgtca tcctggtggg gttgggagcg ctgctttccg ccttcgatcc
      121 ccgaagtcag agcaagtagc cccaggtctg cagcaggtcc ccgtcgcggt gccaccgctc
      181 tccgatgtcc gttcgataga acatgttggg gatgacgatg ttcttcaccc ggttgtaggc
      241 ctcacgacgg gcgtcatcca tggtggaccc ggacccggtg accaccaggg cgtatcccga
      301 gctgcccgcc agccgccagt ccccgtccac cagccggatg tcgcagggat gaacgccacc
      361 gttcaccggt tttcggaaga tcaccgcggc gtcctctgag aactttcgga acgtctcggc
      421 atccacgaag gggaacggcg gaacggcgat gaccacgcag atctggaagc ccttcttggt
      481 ccgcagggtg accggctgtc cccggcccat ccccgtgaga agctcggacc agcggctcag
      541 catgccctcc atctgcaggt tgatggtggg gtagccgaag cggcaggtaa actccagggg
      601 atagatgccc cgagagttgg ctatgcagtt gatgtccacg tacccgcagt agcgcgcggc
      661 cgccagccgg tccttcatgc ggaagagggt ctgctggaac agcgtgctcg atcccatcca
      721 gaagcccgag gtgcccatct ctcccgtctg gggaccgatg tcgtcgttga acatccgctt
      781 gtgctcgaaa ttgatgaagg cgggctgcac gaagtcggtc ccgttgaaga acgcccctac
      841 cgcgacctcc acgccggagg cgtacttctg gagctggaag ctcttgatct tgcttcccca
      901 cccgttgcgg tagtgctcca gcatggtcag cacgtccagg ccgtcttcct cctggcccac
      961 gaaggagagc accttctcgt tctgggcccg gccgctgggc ttgatcacgt agcggcccgg
     1021 gctggccttg atgaaggcga tggcagcgtc gaaggagccg aactcccacc ggggcaacgt
     1081 ggtcaggccg gcggaccgca tctcctcctg cccgaagtcc cgatccatct ccagccggtc
     1141 cgtgtagggc gtgccgccga ccacgcgctt cccctccgcc cggaggcggt cggccagcgt
     1201 gccgaactcg cagtcatcga aaacgatgag gtccgcccac tccttgcagt cctcccatcg
     1261 ctcgatcttc tccacgaacc cgtctcccac gtcccgctcg gacttctgca tgatgtggta
     1321 acgaacgtcg tggccttcct tcttcacttc ccaggcaagg tcatggatga gcccgtactt
     1381 ggagacgaag aggaacctct ggggccgagg ttcccggacc gccggaagtt gggccgcggg
     1441 cggggtgacc ggcagaggag ccggcgctgg aagcgacgga gcctcggtcg cgcccgaccc
     1501 cttgggctgc tcgccgcttg cggcagggtg tgctggctcg ggctggtccg cggaggctgg
     1561 ggaggaaccg ttggcagggg aatcggagcc ggactttccg ccgttctttt tgcctgaggc
     1621 tttttcctct cccatggtgc agcgaacata cgaacccggc gggcggtcgg caagcgattc
     1681 tttgggtata ttgctttgcg atgcccgttg cagccatcag gagataccag agtgagcgcg
     1741 tagtggagaa ccgcgagatc gccccggggc tttttctgct ctcctatcct cggaggcacg
     1801 acttcgttcc gggccagacc gtggcactga ccgtggaccg gtccttcccc gtgcgcttct
     1861 acagcatcgc cagcggcaat tcggaagagc tcatcgaggt gctctacgac ctggtaccgg
     1921 aaggagagct gacgccgaag ctggccggcc tgcggccggg ggatcccatc ctggcatcga
     1981 ctcccttcgg cgccttctgc gattgggacg ggccgtcctg gtgggtcgcc ggcggcacgg
     2041 gcgtggctcc gtttgcctcc atggtccgct ccggcctggt caaggaaaag cggttggtcc
     2101 acggcagccg caaggtggaa tcgctgtatc tgcggcgata cttccaggaa ttgttggggg
     2161 actgctacgt gccctgctgc tcgcgcgagg aggcggaggg cgtctacccg ggccgtcccc
     2221 tggactggct cacgaggaac tatctcccga cccgggcacg atacctggtc tgcggcggaa
     2281 gcctgatggt ggtggccgtc cgggactccc tgatcgaggc ggggattcct ttcggcaacg
     2341 tggtagcc
//
LOCUS       DASRZS010000109         6339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3522392, whole genome shotgun sequence.
ACCESSION   DASRZS010000109 DASRZS010000000
VERSION     DASRZS010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3522392"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFH83_02600"
     CDS             <1..486
                     /locus_tag="VFH83_02600"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HET6485279.1"
                     /translation="KPIQVLFPDSEAVEAMVREKGLNLFYESIENRKACCEVRKVRPL
                     QRELRKHQAWITGLRRSQSPTRTNLHRVEYDEANAMVKVNPLIDWSRERVWGYVREHG
                     LPYNALHDRGFPSIGCLPCTRAVEPGEDERAGRWWWEQPAQKECGIHVQNGTVARRRS
                     S"
     gene            490..1374
                     /gene="cysD"
                     /locus_tag="VFH83_02605"
     CDS             490..1374
                     /gene="cysD"
                     /locus_tag="VFH83_02605"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HET6485280.1"
                     /translation="MDDHLDKLESQSVFIFREAYRKFRNLAMLWSIGKDSTVLLWLVR
                     KAFFGHVPFPLVHIDTSFKIPEMIRYRDELALKWRLNMVYGQNVEALREKRTFPDGRL
                     TRLECCQALKSEALKHTLSGEWTRFRLDHEQKRYVPDPNNEPYTGVIVGVRADEEGSR
                     SKERYFSPRDRHNDWNVDDQPPELWNQFKTDYAPGTHLRIHPLLDWTELNIWEYIHRE
                     SIPVVSLYFDQGSGKRYRSLGCYPCTRPVESTARNVEEIIEELRTGKFSNIAERSGRE
                     QDKEGAGGLEVLRRRGYM"
     gene            1376..3214
                     /locus_tag="VFH83_02610"
     CDS             1376..3214
                     /locus_tag="VFH83_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET6485281.1"
                     /translation="MGLIRGSGEQPLSSAAPREPFNLVVVGHVDHGKSTLIGRLLADT
                     GSLPKGKLDQVRAACERNAKPFEYAFLLDALADEQSQGITIDTARCFFKYKSRDYIII
                     DAPGHIEFLKNMVSGAARAEAALLLIDALEGVKENSRRHGYMLSMLGIRQVVVVVNKM
                     DLVQYVRSTFDRIVEEYTRFLKNIDVAPKAFIPVAAFHGENLISRTPRMPWYAGPSLL
                     DLMDGFTKEKAQAEKAFRFPVQDVYKFTELGDDRRIVAGRVDAGQASVGDDVVFLPSG
                     KKARIVTVEEFNAQKRTWAGAGSSIGFTVEPQVYIRPGEVMARVGEQAPLVGDRLRVN
                     LFWMGRQPMVQGKRYKLKIATAEVPVWLREVNSVLDASELTSVTNKKQIDLYDVADCV
                     LETFKPIAFDRIVEFATTGRFVIVDAYEIAGGGIVLESLENQGSLIEEHIRNRERGWE
                     RTAITPGMRAGQYGQKSTFVVVTGPREAKVAELAKRLEEKLFDHGRFVYFLGLSNALL
                     GIQSDAGIGDDRAEFLRRLGEIAHLFTDAGAILITALADLDDYELAALVALNLPNDIL
                     VVSIGDTELSSAPVGLRLPVDFDLGSGVKAVEELLRSKSILLEHYL"
     gene            3211..3876
                     /locus_tag="VFH83_02615"
     CDS             3211..3876
                     /locus_tag="VFH83_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HET6485282.1"
                     /translation="MSGFGSYIPVGLRVDGRRVVIIGGDGEAALKVEKLLGAGARITV
                     ISASAVPAIESLAGEGAVTLLRRDYRIGDLAKASAAFVCDARFAAAARAEADRRRVLL
                     NVLDRTELCDFIAMAFVARDGLQIAVHSSGKSAALARRIREDLEERYGETFAELVRTL
                     GELRSAVRQMIPSGETRRDFWLDTVDAALVTRAASGAFDRHDFRSRVMAKASSLNASA
                     LHE"
     gene            3854..5713
                     /locus_tag="VFH83_02620"
     CDS             3854..5713
                     /locus_tag="VFH83_02620"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HET6485283.1"
                     /translation="MRLRSTNSPFTSEQADLLDKLLPSLDPHQMTWLAGYLAGLSDVG
                     SPPTAAGAARVAAGTAAAAAPATPPVTVLYASQTGNCAWLAQETARRLEARGFAVTLT
                     TMAATKPRKLKDVSRLLVLVSTQGDGVPPDNGLLFYEDLLSKKAPALEGLRYSVLALG
                     DVSYEDFCTIGRTVDRRLAELGAQPLHPRVDCDLDFQEPSEGWMSGVIEALAREAQPA
                     VSAGAQAGSAAGGVAVATAGSAASAAAVSNSRFHPFESEILENINLNGQGSGKETHHI
                     ELSLEGAGLAFEPGDSLGIYPQNDPHLVDLLIGEMGWRAEEPVPVSKTEERPLRDALA
                     RHYEITVLTRPLLQAAATFSRDGLRELVRPEKDPELQAYVHGRDLLDLARDFSLKGTP
                     ARDFLRTLRRIPPRLYSIASSRAASPDEVDLVIVAVRYESNGRKRSGVFSVQCAERLE
                     PGDRIPIYVNSNPAFRMPADPDAPIIMIGPGTGVAPFRSFLQERQEKGMTGETWLFYG
                     DRRFKTDFLYQVDWLQWLKSGALTRMDVAFSRDGREKLYVQHRMIERSHDLFRWLEDG
                     ASIYVCGDEKSMAPDVNAALERIIAAEGGMSGEKATEYLARLREANRYQRDVY"
     gene            5733..>6339
                     /locus_tag="VFH83_02625"
     CDS             5733..>6339
                     /locus_tag="VFH83_02625"
                     /inference="COORDINATES: protein motif:HMM:NF015425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase"
                     /protein_id="HET6485284.1"
                     /translation="MSESINGQRKLTDVERLKGGSRYLRGTIAEGFQNRVTGAISEED
                     ASLLKFHGMYQQDDRDLRNDRMRQKLEPAFGFMIRVRIPGGVVSTRQWLLLDRLGREY
                     GGDTMRLTTRQTVQLHGLVKWDVRSVVRALHGELLTSIAACGDINRNILCNPNPCQSS
                     AHEDVYQAVLAINRQLLPKTTAYHEIWIDDEKVADSRAAAED"
BASE COUNT         1123 a         2056 c         2137 g         1023 t
ORIGIN      
        1 aagccgatcc aggtgctttt ccccgactcc gaagcggtgg aggccatggt gcgggagaag
       61 ggcctgaacc tgttctacga gagcatcgaa aaccgcaagg cgtgctgtga ggtgcgcaag
      121 gtgcggccgc tccagcgcga gctgaggaag caccaggcct ggatcaccgg tctgcggcgt
      181 tctcagtccc cgacccgcac caacctgcat cgggtggagt acgacgaggc caacgccatg
      241 gtgaaggtca atccgctcat cgactggtcc cgcgagaggg tctggggcta cgtgcgggag
      301 cacggcctgc cctacaacgc gctgcatgac cgagggttcc cgagcatcgg atgtctgccg
      361 tgcacgcggg ccgtcgagcc gggtgaggat gagcgagccg gccgttggtg gtgggagcag
      421 cccgcgcaga aggagtgcgg cattcacgtt cagaacggca ccgtagctcg gcgcaggagc
      481 tcctagacca tggacgatca cctggacaag ctggaatccc agtccgtctt catcttccgc
      541 gaggcctacc gcaagtttcg caacctggcc atgctctggt ccatcggcaa ggacagcacc
      601 gtgctgctct ggctggtgcg caaggcattc ttcggccacg ttccctttcc gctcgtccac
      661 atcgacacct ccttcaagat cccggagatg atccgctatc gtgacgagct ggcgctgaaa
      721 tggcggctga acatggtgta cggtcagaac gtcgaggccc tgcgggagaa gcggacgttc
      781 cccgacggca ggctcacgcg cctggagtgc tgccaggcgc tgaagagcga ggcgctcaag
      841 cacaccctct ccggcgagtg gacgcggttt cgcctggacc acgagcagaa aaggtacgtg
      901 ccggacccga acaacgagcc ctacacgggg gtcatcgtgg gcgtgcgggc cgacgaggaa
      961 ggcagccgct cgaaggagcg ctatttctcg ccgcgggacc gtcacaacga ctggaacgtc
     1021 gacgaccagc cgccggagct ctggaaccag ttcaagaccg actacgcgcc cggcacgcac
     1081 ctccgcatcc accccctgct cgattggacc gagctgaaca tctgggaata catccaccgc
     1141 gagtccatcc ccgtagtgtc cctctacttc gaccagggct ccggcaaacg gtatcggtcc
     1201 ctgggctgct acccgtgcac gcggccggtc gagtcgaccg ccaggaacgt ggaggagatc
     1261 atcgaggagc tgaggacggg gaagttctcg aacatcgcgg aacggtcggg tcgcgagcag
     1321 gacaaggaag gcgcgggcgg cctcgaggtg ctgcgccgcc gcgggtacat gtaggatggg
     1381 tctcatccgg ggttccgggg aacaaccgct ttccagcgcg gccccgcggg aaccgttcaa
     1441 cctggtcgtc gtcggccacg tggatcacgg caagagcacc ctgatcggcc ggctcctggc
     1501 cgataccggc tctctgccga aaggcaagct cgaccaggtc cgcgccgcct gcgagcggaa
     1561 cgccaagccg ttcgagtacg cgttcctgct cgacgccctg gccgacgagc agagccaggg
     1621 catcaccatc gatacggcgc ggtgcttctt caagtacaag tcccgggact acatcatcat
     1681 cgacgctccc gggcacatcg agttcctgaa gaacatggtg agcggcgcgg cccgggcgga
     1741 ggcggcgctt ctcctgatcg acgccctgga aggcgtcaag gagaactcga ggcgtcacgg
     1801 ctacatgctt tccatgctcg ggatccgcca ggtcgtcgtg gtggtcaaca agatggacct
     1861 ggtgcagtac gtccggtcca ccttcgatcg catcgtcgag gaatacacgc ggttcctgaa
     1921 gaacatcgac gtggctccga aggcgttcat cccggtggcc gccttccacg gggaaaacct
     1981 catctcccgt actccccgga tgccctggta cgccggaccg tctctcctcg atctgatgga
     2041 tgggttcacc aaggagaagg cccaggcgga aaaggcgttt cggttccccg tccaggacgt
     2101 ctacaagttt accgagctcg gcgacgaccg acgcatcgtg gccgggcggg tcgacgcggg
     2161 ccaggcgtcg gtaggggacg acgtggtctt cttgccttcc gggaaaaagg ccaggatcgt
     2221 gacggtggag gagttcaacg cccagaagcg gacctgggcc ggggcgggga gctccatcgg
     2281 gttcaccgtg gagccgcagg tctacatccg acccggtgag gtgatggcca gggtcggcga
     2341 acaggctccc ctcgtcggcg accgcttgcg ggtgaacctc ttctggatgg gccgccagcc
     2401 gatggtccag ggcaagaggt acaagctcaa gatcgccacc gcggaagtcc ccgtctggct
     2461 tcgggaagtc aactcggtgc tggacgcctc ggagctcacc tcggtgacca acaagaagca
     2521 gatcgacctc tacgacgtcg ccgactgcgt cctggagacg ttcaagccga ttgccttcga
     2581 ccggatcgtc gagttcgcga ccaccggtcg gttcgtcatc gtcgatgcgt acgagatcgc
     2641 cggtggtggg atcgtcctgg agtctctgga gaaccagggc agcctgatcg aggagcacat
     2701 ccggaatcgg gagcggggct gggagcgcac cgccatcacc ccggggatgc gggccggaca
     2761 gtacggccag aagtcaacct tcgtcgtggt caccgggccc cgggaggcca aggtcgcgga
     2821 gcttgccaag cgattggagg agaagctctt cgaccatggt cggttcgtct acttcctggg
     2881 cctgtcgaac gcactgctgg gcatccagtc cgatgcgggg atcggggatg accgcgccga
     2941 gttcctgagg cgcctcggag agatcgccca cctcttcacc gacgcggggg ccatcctgat
     3001 tacggccctg gcggacctcg acgactacga gcttgcggcc ctcgtggctt tgaacctgcc
     3061 caacgacatc ctggtggtct ccatcggaga caccgagctg agctcggcgc ccgtggggct
     3121 tcgtctgccg gtcgatttcg acctcggctc gggcgtgaag gccgtcgagg agctgctccg
     3181 ctcgaagagc atccttctgg agcactacct gtgagcggct tcggctcgta catcccggtc
     3241 ggcttgcgcg tggacggcag gcgcgtcgtg attatcggag gagacggcga agcggcgctc
     3301 aaggtggaga agctgctggg ggccggggcg cggatcacgg tcatcagcgc ctccgcggtc
     3361 cccgcgatcg agtcgctggc aggggaaggc gcggtaacgc ttctccgccg cgactaccgc
     3421 atcggcgacc ttgcaaaggc atccgccgcc ttcgtctgcg atgcgcggtt tgccgctgcg
     3481 gctcgcgcgg aggcggatcg ccgcagagtg ctcctgaacg tcctggaccg caccgagctg
     3541 tgcgacttca tcgccatggc ttttgtcgcg cgggacggtc tgcagatcgc ggtgcacagc
     3601 tccggtaaga gcgccgccct ggcccggcgc atccgggagg atctggaaga gcggtacggc
     3661 gagaccttcg ccgagctcgt caggaccctg ggggagctca gatccgcggt caggcagatg
     3721 atcccgtccg gagagacgcg acgcgatttc tggctggata ccgtcgatgc ggcgctggtg
     3781 acccgcgcag cctccggcgc gttcgatcgt cacgacttca gaagccgagt gatggcaaag
     3841 gcgagctccc tcaatgcgtc tgcgctccac gaatagcccc tttacctccg agcaggcgga
     3901 cctgctggac aagctccttc cgtccctgga tccccatcag atgacctggc tggccggcta
     3961 cctggcaggc ctcagcgacg tagggtcgcc gccaacggcg gccggggccg cgcgggtggc
     4021 ggccgggacc gccgcggcgg ccgcgccagc aactcctccg gtcacggtcc tgtacgcatc
     4081 ccagaccggc aactgcgcct ggttggcgca ggagacggcg cgccggctcg aagcgcgggg
     4141 cttcgcggtc accctgacga ccatggccgc cacgaagccg cgaaagctca aggacgtctc
     4201 acgcctcctg gtgctcgtga gcacccaggg tgatggcgtt cctcccgaca acggcctgct
     4261 gttctacgag gaccttctct cgaagaaggc gcctgccctg gaggggcttc gatattcggt
     4321 gctggcgctg ggggacgtgt cctacgagga cttctgcacg atcgggagga ccgttgaccg
     4381 gcggctggcc gagcttggcg cccaacccct gcacccccgg gtcgattgcg atctcgattt
     4441 ccaggagccg tccgagggat ggatgagcgg ggtgatcgag gccctggccc gggaagctca
     4501 gccggcggtc tccgccggag cgcaggccgg atccgccgcc ggcggggttg ctgtcgcgac
     4561 agccggcagc gcggcctccg cggcagccgt ctccaattcg cgcttccatc ccttcgaatc
     4621 ggagatcctg gagaacatca acctgaacgg ccaggggtcc ggcaaggaga cccatcacat
     4681 cgagctgtcc ctggaggggg ccgggctcgc cttcgagccg ggagactcgc tcgggatcta
     4741 tccgcagaac gaccctcacc tcgtcgatct gctgatcggc gaaatggggt ggcgcgcgga
     4801 agagcccgtg ccggtgtcga agaccgagga gcgaccgctt cgggacgcgc tggcgcggca
     4861 ctacgagatc accgtgctca cccggccgct gctgcaggcc gccgcgacgt tcagcaggga
     4921 cggcctgcgc gagctcgtga ggcccgagaa ggacccggag ctccaggcct atgtccatgg
     4981 acgggacctg ctcgacctgg cccgggactt cagcctgaag ggtacgccgg ctcgagactt
     5041 tctgcggacc ctccgcagga tcccaccccg gttgtactcc atcgcctcca gccgcgcggc
     5101 gagccccgac gaggtggatc tggtcattgt ggcggtgcgc tacgaaagca acggccgcaa
     5161 acgcagcggg gtcttctccg tccagtgcgc cgagcgcctg gagcccgggg accgcatccc
     5221 catctacgtg aactcgaacc cggcgttccg catgccggcg gatccggatg ccccgatcat
     5281 catgatcgga ccgggcaccg gcgtggcgcc gttccgatcc tttctccaag agcgccagga
     5341 aaagggcatg acgggagaga cctggctctt ctacggcgac cggcgcttca agaccgactt
     5401 tctctaccag gtcgactggt tgcagtggct gaagagcggc gccttgaccc gcatggacgt
     5461 ggccttctct cgcgacgggc gcgagaagct ctacgtgcag catcgcatga tcgagcgaag
     5521 tcacgacctg ttccgctggc tggaagacgg cgccagcatc tacgtatgcg gtgacgagaa
     5581 gagcatggcc ccggacgtca acgccgcact ggaacgcatc atcgccgcgg aagggggcat
     5641 gagcggcgag aaggcgacgg agtaccttgc gcggctgcgt gaggccaacc gctaccagcg
     5701 ggacgtctac tgaccgcgtc gagagggacg agatgagtga atcgatcaac ggccagagga
     5761 agctgacgga cgtggagcgg ctgaagggcg ggagccggta cctgcggggg accattgccg
     5821 aaggtttcca gaaccgggtt accggtgcga tctccgaaga ggacgccagc ctcctcaagt
     5881 tccacggcat gtaccagcag gacgaccggg acctgcgcaa cgaccgcatg cggcagaagc
     5941 tcgagccggc cttcgggttc atgatccgcg tgcggattcc cggcggcgtg gtgagcaccc
     6001 gccagtggct tctcctggac cgtctgggtc gtgagtacgg cggggacacc atgcgcctca
     6061 cgacgcgcca gaccgtgcag cttcacggtc tggtgaaatg ggacgtgcgc tcggtggttc
     6121 gggccctcca cggggagctc ctgaccagca tcgccgcctg cggagacatc aaccgcaaca
     6181 tcctctgcaa cccgaacccg tgccagtcct ccgcgcacga ggatgtctac caggcggtcc
     6241 ttgccatcaa tcgccagctc cttccgaaga cgactgccta ccatgagatc tggatcgacg
     6301 acgagaaggt ggccgacagc cgcgccgccg cggaggacg
//
LOCUS       DASRZS010000110         5248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1214316, whole genome shotgun sequence.
ACCESSION   DASRZS010000110 DASRZS010000000
VERSION     DASRZS010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214316"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFH83_02630"
     CDS             complement(<1..270)
                     /locus_tag="VFH83_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485285.1"
                     /translation="MRKTTALLTIAILLATGGVMAAFAGGKAETGGGGGTLPPVTLTM
                     IIPGPPQKDQQRVDDAISKYLQPTLNVKLNIQQYDYSGWNDRSNLV"
     gene            complement(318..1169)
                     /locus_tag="VFH83_02635"
     CDS             complement(318..1169)
                     /locus_tag="VFH83_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017689303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485286.1"
                     /translation="MLHALLGLFAILCFVPLINVLSVSFSDQKSILQFGYRLVPKSWS
                     GQAYRFLWDDIAQIGRSYGISAIVTAVGTFASIVLTALYAYPLSRRDFPYRKGFAIFL
                     FITMLFNGGLVPFYVLYTQYLHVRDSLAALILPYLVNPFYVIIMRTYITSSVPDSLIE
                     AAKLDGASEPYILMRIVFPVALPVFATIALFVSIVYWNDWFMSLLFISNPRNVSIQFY
                     MYKTILNIQYMLINSNLAARMDISKIPTETVRMAMAIVGIGPIVFAYPFFQRYFIKGL
                     TLGAVKG"
     gene            complement(1210..2130)
                     /locus_tag="VFH83_02640"
     CDS             complement(1210..2130)
                     /locus_tag="VFH83_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6485287.1"
                     /translation="MAQGFTHTIGKHRALLLMLLAPVLYLLINNYLPMIGVIIAFKNI
                     NYAKGIFRSDWVGFYNFKYLFATRDAFIITRNTLLYNLLFIVLNVVIPVSVAIAISEL
                     RGSRLKRLYQTAFLFPYFISMVVVSYLVLSLLSPDKGLLNETLLPFLRRAPINWYNET
                     IYWPFILAIVYVWKNAGYTVVIYLATIAGIDTQYYDAVAIDGGSKWVQIRYVTLPLLQ
                     PTIAVLTLLAVGKIFNSDFGLFYQVPLQSGTLFPVTQVIDTYVYRALIQSSDLGMASA
                     AGLYQAVVGFVLVFITNAIVRRANPELALF"
     gene            2212..3036
                     /locus_tag="VFH83_02645"
     CDS             2212..3036
                     /locus_tag="VFH83_02645"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HET6485288.1"
                     /translation="MNVFIMTDMEGVAGVLNHDDWVLPTGRWYEKGQRFLTQEVNAAV
                     EGLFAAGARDIVVADAHGAGGIDPEALDPRASLQRGFPDPWPFGLDASFHAVVWIGQH
                     AKAGTPLAQIPHTEWFNTLDARLNGVSVGEFGMLAFCAASLGVPTLFGSGDQAFCEEA
                     RTLVAGIETVAVKRGLTPGSGSEMDTDTYRKRNLAAVHLQPQRARALIREGAERALRR
                     LGREPSAFLPPVLTAPFRLEMDYRDFGSIPAHRTVREHPSDVIRCMNAPQRTVEAG"
     gene            complement(3044..3898)
                     /locus_tag="VFH83_02650"
     CDS             complement(3044..3898)
                     /locus_tag="VFH83_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485289.1"
                     /translation="MLDSHVQRTALGDVLKLRLRSAPFPHASRQNGYRYHDTQYQADP
                     HYVDSTVAVFIPPGIRRDLPVNLVFFFHGWNSSVDDVQQRFDLYRQFAQSGVPALLVL
                     PELAWNAPDSSGGKLEDPGGFTRMVTELLEALRGTGEIGISAPGNIVLAGHSGAYRVI
                     AQILTNADLAANVKEVWLFDALYDFADQYGAWIDRAATRFVSISASDGEQTTDVDKLI
                     ASLREEGVACDLERDDPLIDPWTLHSRVVFLKSDTDHFGVVAARREFRRLLETSPALA
                     AAPPAIAM"
     gene            4230..5042
                     /locus_tag="VFH83_02655"
     CDS             4230..5042
                     /locus_tag="VFH83_02655"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6485290.1"
                     /translation="MPVTSAWVHYGDQIGFFAAPDHAATPLPSVVVIQEVWGVNDQIE
                     DVTRRIAASGYAALAPDVYAVNGQRPEALNRERVEEVVAFGATLPPGSRFDPAAREAA
                     LAKLPENQRQRLSESFAKMFSMPGAMQGFVGSLRRAVRYLREERPESKGRKVGCVGFC
                     MGGGLSALLACEEPDLSAAAVFYGNAPSAEKVAGIRCPVIGFYAGTDQRVNAGIPAFE
                     SAMKKEGKRFEYRIYEGASHGFFNDSGPVYEVNAARDSFARLLRFFADHLVT"
     gene            5053..>5248
                     /locus_tag="VFH83_02660"
     CDS             5053..>5248
                     /locus_tag="VFH83_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HET6485291.1"
                     /translation="MPAVSECLVMAKPIGPRCNMRCGYCYYLSREGFIPRHPGRMPLE
                     TMERYILQRLEASPGPATHFE"
BASE COUNT          975 a         1582 c         1783 g          908 t
ORIGIN      
        1 gaccaggttg cttcggtcgt tccacccgct gtagtcgtac tgctggatgt tgagcttgac
       61 gttcaacgtg ggctgcaggt acttgctgat ggcgtcatcg accctctgct ggtccttctg
      121 cggtgggccg gggatgatca tggtgagcgt gaccggagga agcgtgcctc cgccaccgcc
      181 cgtctccgcc ttgcctccgg cgaacgccgc catgacgccg cccgtcgcca acaggatggc
      241 gatcgtcagg agtgctgtag ttttcctcac tggaaacctc caaaagctag aatgagtctc
      301 cgctgacagc ggatctgcta cccctttacc gcgcccagcg tcagcccctt gatgaagtac
      361 cgctggaaga acgggtacgc aaagacgatg ggcccgatcc ccacgatggc catggccatc
      421 cggacggtct cggtggggat cttcgagatg tccatccggg ctgccaggtt gctgttgatc
      481 agcatgtact ggatgttgag gatcgtcttg tacatgtaga actggatgct cacgttcctg
      541 ggattggaga tgaacagcag gctcatgaac cagtcgttcc aatacacgat ggacacgaag
      601 agcgcgatgg tagcgaacac cggcagcgcc acgggaaaga cgatgcgcat caggatatag
      661 ggctccgagg cgccgtccag cttggccgct tcgatgaggg aatccgggac gctgctggtg
      721 atgtaggtcc gcatgatgat gacgtagaac gggttcacca ggtacggaag gatgagcgcc
      781 gccagggaat cccggacgtg caggtactgg gtgtacagca cgtagaacgg gaccaggccg
      841 ccgttgaaga gcatcgtgat gaagaggaag atggcgaacc ccttgcggta ggggaaatcc
      901 cttcgggaga gcggatacgc gtacaacgcc gtgagcacga tgctggcgaa cgtcccgacg
      961 gcggtgacga tcgcggagat cccgtaggag cgcccgatct gcgcgatgtc gtcccacagg
     1021 aagcggtacg cctgcccgct ccaggacttg ggaaccagcc gatagccgaa ctggaggatc
     1081 gacttctggt ccgagaagga gaccgacagg acgttgatca gcgggacaaa gcacaggatg
     1141 gcgaacaggc ccaacagggc gtgcagcacg acgttggccg gcgtggagat ctcgttccat
     1201 ccgcggcgtc tagaagagag ccagttcagg gtttgccctc ctcacgatgg cgttggtgat
     1261 gaagaccagc acgaacccga ccaccgcctg gtagagcccg gcggcggatg ccatgcccag
     1321 atcgctcgac tggatgagcg cccggtagac gtaggtgtcg atcacctggg tcaccggaaa
     1381 gagcgttccg gactggagag ggacctggta gaacagaccg aagtcggagt tgaagatctt
     1441 cccgaccgcc agcagcgtca gcacggcgat ggtcggctgg aggaggggca gcgtgacata
     1501 gcggatctgc acccacttgc tgccgccgtc gatggctacc gcgtcgtagt actgcgtatc
     1561 gattcccgct atggtcgcca ggtagatcac gaccgtatag cccgcgttct tccagacgta
     1621 cacgatggcc aggatgaacg gccagtagat cgtctcgttg taccagttga tcggcgcacg
     1681 ccgcaggaaa ggcaacaggg tctcgttcag gagccccttg tcgggagaga gcagggacag
     1741 cacgaggtag ctgaccacca ccatcgagat gaagtacggg aacagaaagg cggtctggta
     1801 gagccgcttc agcctgcttc ctcggagctc gctgatcgcg atggccacgc tcacggggat
     1861 cacgacgttc aggacgatga acaggaggtt gtagagcagc gtgttccgcg tgatgatgaa
     1921 cgcgtcccgc gtagcgaaca ggtacttgaa gttgtagaag ccgacccagt cgcttcgaaa
     1981 gatccccttg gcgtagttga tgttcttgaa cgcgatgatg accccgatca tcggcaggta
     2041 gttgttgatg agcagataga gcaccggcgc cagaagcatg agaagcaggg cccggtgctt
     2101 cccaatcgtg tgcgtaaagc cctgtgccac ggtaggggat gctaacgatg ctccctccgc
     2161 atgtcaacga cgcgttccgc atggtatcgt cccctccgcg gaggctgaac gatgaatgtg
     2221 ttcatcatga cggacatgga gggcgtggcg ggcgtgctca accacgacga ttgggtcctg
     2281 ccgacggggc gctggtatga gaagggccag cggtttctga cgcaggaggt gaacgcggcc
     2341 gtggaggggc tgttcgcggc tggcgcgcgg gacatcgtcg tggcggatgc gcacggtgcc
     2401 ggtggcatcg atccggaagc cctggacccg cgcgccagcc tgcagcgcgg gttcccggat
     2461 ccctggccct tcggcctgga cgcgagcttt cacgcggtgg tgtggatcgg gcagcatgcc
     2521 aaggcgggga cccccctggc ccagatccct cacacggaat ggttcaacac tctggatgcg
     2581 cgcctgaacg gcgtctccgt cggggagttc ggaatgctgg ccttctgcgc ggcgtccctc
     2641 ggggtgccga ccctcttcgg cagcggtgac caggcattct gcgaggaagc ccggaccctg
     2701 gttgccggga tcgagacggt cgccgtcaag cgcggcctga ccccgggcag cgggtcggaa
     2761 atggacaccg atacctatcg aaagcgcaat ctggccgccg tgcacctcca gccccagcgg
     2821 gctcgggccc tcatccgcga gggagcggag cgcgcgctgc ggcgtttggg cagggaaccg
     2881 agcgcgttcc tcccacccgt cctcacggcg ccgttccggc tggagatgga ctaccgggac
     2941 ttcgggtcga ttccggcgca tcggaccgtc cgagagcatc cgtcggacgt gatccgctgc
     3001 atgaatgcgc cgcagcgcac ggtagaggcc ggatagccgg tggctacatg gctattgcgg
     3061 gaggggcggc cgccagcgct gggctcgtct ccaggagcct tcggaactcg cgacgggcgg
     3121 ccacgacgcc gaagtggtcg gtgtcgctct tcagaaagac cacgcgggag tgcaacgtcc
     3181 acggatcgat gaggggatcg tcgcgctcca ggtcgcaggc aacgccctct tcccggagcg
     3241 aggcgatgag cttgtccacg tcggttgtct gctccccatc ggaggcgctg atggagacga
     3301 atcgggtggc ggcgcgatcg atccaggcgc cgtactggtc cgcgaagtcg tacagggcgt
     3361 cgaagagcca tacctctttg acgttggccg cgagatccgc gttcgtcagg atctgggcga
     3421 tcacccggta ggccccgctg tgtcccgcaa ggacgatgtt gccgggcgcc gagatgccga
     3481 tctcaccggt cccccgcagg gcctccagga gttccgtgac catccgggtg aaccctcccg
     3541 ggtcctccag cttgccgccg ctggagtccg gggcgttcca ggcgagctcc ggcaggacga
     3601 gaagcgccgg gacgccgctt tgcgcgaact ggcgatacag gtcgaagcgc tgttggacgt
     3661 cgtcgacgct ggagttccag ccgtggaaga agaagaccag gttgaccggc aggtcgcggc
     3721 gaatccctgg tgggatgaag accgccacgg tagaatccac gtagtgcgga tccgcctggt
     3781 actgggtgtc gtggtaccgg tacccgttct ggcggctggc gtgcggaaaa ggcgcggacc
     3841 gcaatctcag cttcagaacg tcgccgagag cggtgcgctg gacgtgcgaa tccagcatat
     3901 gactcacgtg ccggcgcggt cccggtgccc caccgcggtt ctgcgaggag gacaggccgg
     3961 tcaccgcaac gatgaggagc agacccacgc tggttcgagc ggccgagcca acccttctcc
     4021 cggaccctct ggcgggactc tctgagattc ggcgatccat ctggcccaga gtatcggggc
     4081 tgatccgcgc gaaacccata atcgggacga tggttccaag gccgaatctt cacggtcctt
     4141 cgcgtcgaag aaattcgact tgacgcggac cggcggacgg agtctccttg tagcgagcat
     4201 ccccccaggc aacggaaagg aaggcagtca tgcccgtgac gagcgcatgg gtccactatg
     4261 gggaccagat cggcttcttc gcggcccccg accacgccgc gacgcccctg cccagcgtgg
     4321 tggtcatcca ggaggtctgg ggagtcaacg accagatcga agacgtgacc cgaaggatcg
     4381 ccgcctcggg gtacgcggcg ctggctccgg acgtgtatgc cgtcaatggc cagcggcccg
     4441 aagccctcaa ccgggaacgg gtggaggagg tagtcgcctt cggggcaacg ctgcctcctg
     4501 gctctcgctt cgacccggct gcccgggagg ctgccctggc gaagctgccc gagaaccagc
     4561 gccagcggct gagcgagagc ttcgcgaaga tgttctccat gcccggcgcg atgcaggggt
     4621 tcgtcggatc gctccgccgc gcggttcggt acctcaggga agagcggccg gagagcaaag
     4681 gacgaaaggt cggctgcgtg gggttctgca tgggaggcgg tctttccgcg cttcttgcct
     4741 gcgaggagcc ggacctctcc gccgccgcgg tattctatgg caacgctccc tccgcggaga
     4801 aggtggcggg catccgctgc cccgtgatcg ggttctacgc gggcaccgac cagcgtgtga
     4861 acgccggcat accggccttc gagtccgcca tgaagaagga aggaaagagg ttcgagtacc
     4921 ggatctacga gggcgccagc cacggtttct tcaatgacag cggtccggtg tatgaggtga
     4981 acgccgcccg ggattccttt gcgcgtctct tgcggttctt cgcggaccac ctggtgacct
     5041 aggctcccgg ggatgcccgc ggtttccgaa tgtctcgtga tggccaagcc catcggtccc
     5101 cggtgcaaca tgcgctgcgg gtactgctac tacctgtccc gggaagggtt catccccagg
     5161 cacccgggcc gcatgcccct ggaaacgatg gagcggtaca tcctccagag gctggaagcc
     5221 tcaccgggtc ccgctacgca cttcgagt
//
LOCUS       DASRZS010000111         2556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3908765, whole genome shotgun sequence.
ACCESSION   DASRZS010000111 DASRZS010000000
VERSION     DASRZS010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3908765"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFH83_02665"
     CDS             <1..345
                     /locus_tag="VFH83_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6485292.1"
                     /translation="VDLALYDAAIVDGAGHLRRLWHVTLPSLRGIIVVLALLNLSEIL
                     NAGFDQIFNIYSPVVYQSGDIIDTFVYRTGLQNAQYAVAAAAGFFKSIVSLVLLSSSY
                     WMAYRLADYRIF"
     gene            397..1245
                     /locus_tag="VFH83_02670"
     CDS             397..1245
                     /locus_tag="VFH83_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485293.1"
                     /translation="MVFNVIFFFLLAMVCFLPMINVLAVSFSSASAAQAGFVSFWPVH
                     FNVASYKYALGRPQFLTSMWVSLKRILLGGLINMLLTILAAYPLSKDNTKLRMRTVYS
                     WFFVTTMLISGGLIPTFMVINKLGLLDTIWALVLPGALPVFNLIILLNFFRQIPVELE
                     EAAAIDGASHWTILWRIYVPCSTPAIATLTLFVVVGHWNSWFDGLIYMNHTSHYPLQS
                     YLQTVIVQRNAADMQNIDYRDLALMSDRTIKASQVFMGAVPILLAYPFLQRYFVTGIT
                     LGSVKG"
     gene            1261..2037
                     /locus_tag="VFH83_02675"
     CDS             1261..2037
                     /locus_tag="VFH83_02675"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HET6485294.1"
                     /translation="MKIYIHTDLEGISGIDTAGVEEKNHPGYRHAVERLMSDLNAAVD
                     GAFAGGATHVTVLDSHGGGGNFDLSLLDKRADNDPRANKQWWGKLDDTYAGTFFIGAH
                     AMAGTQNGFLDHTDDSLRWLNYYVNNRRMGELGMWAMVAGHFNVPMLMVAGDEAACVE
                     ARQFFDPVECAAVKRGVGRSRAILVDPSEATERIRAAARQALRLVGTARPFKPHLPME
                     VKLELTRSDYCDALAENPRNERLDARTVRKFCASYLELAL"
     gene            2068..>2556
                     /locus_tag="VFH83_02680"
     CDS             2068..>2556
                     /locus_tag="VFH83_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485295.1"
                     /translation="MKDFGASGARWIWNDRGLPRENHYVEFRREIVLDRPPSRDATLY
                     LSSDSNYVLWINGAFVDCGQYSDFPNAKVFDALSVGKHLRQGKNTLCVLVYHYGKTCH
                     VYLKGDPGLVYVLETGGVRIPSDAETWCRTSRAYTSGPVYQINQFILFGFHYGADKED
                     DWT"
BASE COUNT          468 a          804 c          800 g          484 t
ORIGIN      
        1 gtagaccttg ccctctacga cgcggcgatc gtggacgggg cgggccacct gagacggctc
       61 tggcacgtca cgctcccgag cctccgcggc atcatcgtgg tcctggctct gctgaacctc
      121 agcgagatcc tgaacgccgg cttcgaccag atcttcaaca tctacagtcc ggtcgtctac
      181 cagagcggag acatcatcga tacgttcgtt taccggaccg gcctgcagaa cgcccagtac
      241 gcggtggccg cggccgccgg tttcttcaaa tccatcgtgt cgctggtgct gctgtcctcg
      301 tcctactgga tggcgtaccg gctcgccgac taccggattt tctgaggcag gggaagggcg
      361 atgcactaca agggttccat cagccgacgg gtcttcatgg tcttcaacgt gattttcttc
      421 ttcctcctgg cgatggtctg cttccttccg atgatcaacg tactggcggt ctccttcagc
      481 tccgccagcg cggcccaggc agggttcgtg tccttctggc cggtccattt caacgtggcg
      541 tcctacaagt acgccctggg gagaccgcag tttctgacct ccatgtgggt gtctctgaag
      601 cggatcctcc tgggcggcct catcaacatg ctgctcacca tcctggcggc gtacccgctt
      661 tccaaggaca acaccaagct ccggatgcgg acggtgtata gctggttctt cgtaacgacg
      721 atgctcattt ccggcgggct catcccgacg ttcatggtca tcaacaagct gggcctgctg
      781 gacaccatct gggcgctggt gctgcccgga gcgctgccgg tcttcaacct gatcatcctt
      841 ctcaacttct tccggcagat ccccgtcgag ctggaagagg ccgcagcgat tgacggagcc
      901 agccactgga ccatcctgtg gaggatctac gtcccgtgct ccacgcccgc catcgccacg
      961 ctgacgctgt tcgtcgtggt aggccactgg aactcctggt tcgacggcct gatctacatg
     1021 aaccatacct cgcactatcc tctgcagagc tacctgcaga cggtcatcgt gcagaggaac
     1081 gccgccgaca tgcagaacat cgactatcgg gacctggcgc tcatgtcgga ccggaccatc
     1141 aaggcgtccc aggtcttcat gggcgccgtg ccgatcctgc tggcgtaccc gttcctgcag
     1201 cggtacttcg tgaccgggat caccctggga agcgtgaagg ggtaagaggg atctgaaagc
     1261 atgaagatat acatccatac ggacctcgag gggatcagcg ggatcgatac ggccggagtg
     1321 gaagaaaaga accatcccgg gtatcgccac gcggtggagc ggctcatgtc cgacctgaac
     1381 gcggcggtgg acggagcctt tgcgggtggg gcgacgcacg tgaccgtgct ggattcccac
     1441 ggcgggggcg gcaacttcga cctttccctg ctggacaaac gcgcggacaa cgatccacgg
     1501 gcgaacaagc agtggtgggg aaagctcgac gatacgtatg ccgggacctt cttcatcggc
     1561 gcgcacgcga tggccgggac ccagaacggg ttcctggacc acaccgatga cagccttcgc
     1621 tggctgaact actacgtgaa caaccgccgg atgggcgagc tcgggatgtg ggccatggtc
     1681 gcggggcact tcaacgtccc catgctcatg gtggcggggg acgaagcggc ctgcgtggag
     1741 gctcgccagt tcttcgaccc ggtcgagtgc gcggcggtca agcggggggt gggcaggagc
     1801 cgcgccatcc tggtggaccc gagcgaggcc acggagcgca tacgcgcggc ggcccgtcag
     1861 gccttgcggc tggtcggcac ggctcgaccc ttcaagccgc acttgcccat ggaagtcaag
     1921 ctggagctca cgcgcagcga ctattgcgac gcgctggccg agaacccgcg caacgaacgg
     1981 ctggacgcca ggaccgtgcg caagttctgc gccagctacc tggagctcgc gctctagtcg
     2041 ccgccgctga ccctgggaga acaacccgtg aaagactttg gcgcctcggg ggctcgctgg
     2101 atctggaatg accgcggact gccgcgggag aaccactacg tcgagttccg tcgtgagatc
     2161 gtgctggatc ggcctccgtc ccgggatgcc accctctatc tgtcgtcgga ctccaactac
     2221 gtgctctgga tcaacggcgc gttcgtcgac tgcgggcagt acagcgattt cccgaacgcc
     2281 aaggtattcg acgcgctgag cgtcgggaag catctgcggc agggaaagaa caccctgtgt
     2341 gtcctggtct atcactatgg aaagacctgc cacgtctatc tgaaggggga tccagggctc
     2401 gtctatgtgc tggagaccgg gggcgtccgg atccccagtg acgcggagac ctggtgcagg
     2461 acctccaggg catataccag cgggccagta taccagatca accagttcat cctgttcgga
     2521 ttccactacg gcgcggacaa ggaggacgac tggacc
//
LOCUS       DASRZS010000112         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1214654, whole genome shotgun sequence.
ACCESSION   DASRZS010000112 DASRZS010000000
VERSION     DASRZS010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214654"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VFH83_02685"
     CDS             complement(<1..1078)
                     /locus_tag="VFH83_02685"
                     /inference="COORDINATES: protein motif:HMM:NF021632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HET6485296.1"
                     /translation="MSAKVDLRAIEREIEQIREREANPYSAGTRTNLFTLLIFRAEGA
                     SQQPKDRAGAALAHLLGRRPARIITFERSQSLESELSVSGRCSPDRLNRGVCFEEVYI
                     QCGDDGVGSDPGTWAPLLIRDLPTFAWWPDGLATGILPVEDPIAGVSDRVDKVIIDSA
                     AAQRTGADAGEASPAQAPFDAAGMAVGSPAPFDIPTDTRSFPAELGLLLKTRDTKGGS
                     YLVTDFAWRRGQALREQSARAFDPPEHRELISRIRGVRLRGGMRAEAQLYFHWLAQRL
                     RWTRGGSRDGLPVVWDQARRDIRLSHDFEGPLAEGFVVTFSFEGAPDLPVGCTRGGCV
                     SIDDEKSAYRFPTDGEILLQEVDSL"
     gene            complement(1075..2565)
                     /gene="zwf"
                     /locus_tag="VFH83_02690"
     CDS             complement(1075..2565)
                     /gene="zwf"
                     /locus_tag="VFH83_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET6485297.1"
                     /translation="MQENPLRVGLGLPREPQPLSIIIFGASGDLTRRKLMPALFSLYR
                     KGSIPGVKIIGFARREWDVAAFRKVVLESLEGEGGDKEVQEAFVQGVDYVCSPFESST
                     GYQAIAERFPQPASRIFYLATPPDSYESIVDHIHQAGLADQSRGFSRIIVEKPLGRDS
                     ESARMLNQSIARVFPEEQIYRIDHYLGKETVQNLMLMRFGNGIFEPVWSNRYIDHVQI
                     TMSETIGVGTRGPYYESAGALRDMMQNHMLQLLCLVTMEPPVDLAPDSIHNEKVKVLR
                     SITPIRADRVAELSVRGQYERGIVDGEEAPGYRQETGVSRTSSTETFAALRVSLDSWR
                     WAGVPFLLRTGKRLSRRTTEISIHFKRPPLLLFAGREASATPPNVLIFRIQPEEGITI
                     GFNSKTPGPTTDMRLVNMDFAYGSAFAEELPEAYERLLLDAMLGDSTLYMRKDEVESA
                     WSFITEILDAWKSTGRPAVEGYRAGSSGPDAAVRLTGSPDRRWRRL"
     gene            2593..>2781
                     /locus_tag="VFH83_02695"
     CDS             2593..>2781
                     /locus_tag="VFH83_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485298.1"
                     /translation="METRSGFEGTWANPWPGALLQVKEETRSTMEDAFELARSGAASG
                     SVAVAGFQSLGRGRVPGRT"
BASE COUNT          448 a          925 c          934 g          474 t
ORIGIN      
        1 ccagggagtc cacttcctgg aggaggatct cgccgtcggt ggggaatcga taggcgctct
       61 tctcgtcgtc gatcgaaacg cagccccctc gggtgcagcc caccggcagg tccggcgccc
      121 cttcaaagga gaaggtgacg acgaagccct cggcgagggg accctcgaag tcgtggctca
      181 gccggatatc ccgacgggcc tgatcccaga caacggggag accgtcacgg ctcccgcctc
      241 gagtccagcg caggcgctgg gccagccagt ggaagtagag ctgcgcctcc gcgcgcatgc
      301 cgcccctcag ccggaccccg cggatgcggg agatcagctc gcggtgctcc gggggatcga
      361 acgcccgcgc gctctgctct cgaagcgcct ggccccgcct ccacgcgaag tccgtcacca
      421 ggtagcttcc gcccttggtg tcccgcgtct tgaggagcag accgagctcg gcggggaagc
      481 tccgggtgtc cgttgggata tcgaagggag ccggcgagcc gaccgccatg cccgcggcat
      541 cgaagggtgc ctgggccggg gacgcttctc ccgcgtctgc tcccgtccgc tgggcggcgg
      601 cgctgtcgat gatcaccttg tccacccgat ccgagactcc ggcaatcgga tcctccacgg
      661 ggaggatccc cgtggccagg ccgtccggcc accaggcgaa ggtgggcagg tcccggatca
      721 gcaggggcgc ccaggtgccc ggatccgaac ctacgccatc gtccccgcac tggatgtaga
      781 cctcttcgaa acagacgccc cggttgagcc ggtccggaga gcagcggccg ctcacggaca
      841 gctcgctctc cagggactgg gaccgctcga aggtaatgat ccgcgccggg cgacggccca
      901 agaggtgggc cagcgcggcc cccgcccggt ccttgggctg ctgggaggcg ccctcggccc
      961 ggaagatcag gagcgtgaag aggttggtcc gcgtgccggc ggagtacggg ttcgcctccc
     1021 gttcccggat ctgttcgatc tccctctcga tggcgcgaag gtccactttg gcgctcatag
     1081 tctccgccac cttctgtccg ggctgccggt caaccggacc gcggcgtccg ggcccgagct
     1141 cccggcgcgg tagccttcca cggccggccg ccccgtgctc ttccaggcat ccaggatctc
     1201 cgtgatgaag ctccaggcgg actccacctc gtccttccgc atgtagaggg tggaatctcc
     1261 cagcatcgca tccaggagga gccgctcgta ggcctccgga agctcctccg cgaacgccga
     1321 gccgtaggcg aagtccatgt tcaccagccg catgtcggtg gtcggccccg gcgtcttgga
     1381 gttgaacccg atggtgatcc cttcctcggg ctggatgcgg aagatgagca cgttgggggg
     1441 cgtggcgctg gcctcccgac cggcgaaaag gagcagaggc ggtcgtttga agtggatgga
     1501 gatctccgtg gtccgccggg agaggcgctt gccggtgcgc agcaggaacg ggacgccggc
     1561 ccatcgccag gaatccaggc tgacgcgcag ggcggcgaag gtctccgtgg aggaggtgcg
     1621 ggagactccg gtctcctgcc gatatcctgg cgcttcctcg ccgtccacga tgcctcgctc
     1681 gtactggcct cggaccgaga gctccgccac gcggtccgcg cggatgggcg tgatggaccg
     1741 aagcaccttc accttctcgt tgtggatgct gtccggggcc aggtcaacgg gcggctccat
     1801 cgtcaccagg cacaggagct ggagcatgtg gttctgcatc atgtcccgaa gcgcccccgc
     1861 gctctcgtag taaggtccgc gggtgcctac gccgatggtc tcggacatcg tgatctgcac
     1921 gtggtcgatg taccggttgc tccataccgg ctcgaagatc ccgttgccga acctcatcag
     1981 catgaggttc tggacggtct ccttgcccag gtaatggtcg atgcggtaga tctgctcttc
     2041 gggaaagacc cgcgcgatgc tctggttcag catccgggcg ctctcactgt cccggcccag
     2101 gggcttctct actattatac gggagaaccc acgggactgg tccgccagac ccgcctggtg
     2161 gatgtggtcc acgatgctct cataggagtc cggcggagtg gccaggtaga agatccggct
     2221 ggcaggttgg ggaaatcgct cggcaatagc ctggtacccc gtggaagact cgaacggcga
     2281 gcagacatag tccacgccct ggacgaacgc ctcctggacc tctttgtctc ccccctcacc
     2341 ctcaaggctc tccaggacca ccttgcggaa ggcggccacg tcccactccc ggcgcgcgaa
     2401 gcctatgatc ttcacgcccg ggatgctccc cttgcgatag agggagaaga gggccggcat
     2461 cagttttctc cgggtcaggt ctccgcttgc cccgaagatg atgatggaca aaggctgggg
     2521 ttctcggggc aggcccaacc cgactcgtag tgggttctcc tgcattttca aaatggtaca
     2581 taatagcccc ccatggagac aaggagtgga ttcgaaggca catgggccaa tccctggcct
     2641 ggggcgctcc tccaggtgaa ggaagagacg cgttccacga tggaagatgc gttcgagctg
     2701 gcgcgctcgg gcgccgcgtc cgggagcgtc gccgttgccg gcttccagag cctgggacgc
     2761 ggccgggtgc cgggccgcac g
//
LOCUS       DASRZS010000113         4633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2368643, whole genome shotgun sequence.
ACCESSION   DASRZS010000113 DASRZS010000000
VERSION     DASRZS010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2368643"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1786)
                     /locus_tag="VFH83_02700"
     CDS             complement(<1..1786)
                     /locus_tag="VFH83_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485299.1"
                     /translation="MRKRLVLLVLIGLALAAIGWAQETTWINPFTLSAPSSMLSQASS
                     GLFYDELDILFRSPAELSNYKGINLYTSYGNFEHNWILNQGISPFTGTGVVNPFSTTA
                     PTQQGGGANIGTYLFGFTMPVASYRMGAAGGFVLGQTDLLNIGGLTDFWTRSNTGTVD
                     ANANNQPDYTFTNTESYTDLTTNATVNVTGGFDLNPLGVSLWAYLNGQSRNIGGSYAY
                     ARTQGTDAGTPLPADTVSTKNVFFGTADGKPAAYPVPAGTTWTVQANGEMPLSVGGVS
                     MPVTATVLVGGQPYSTVAFAYSPLASFNFTTVNAAGVAAKTSSLTFNEGLGTTNPDTS
                     AGVIPNQTTLTQANLRAAVTADLGTLTGGALALDTKNYANSTFMVGLGGNIDPVLKVS
                     DQLKFRTRGAVSYTIGFQNYKVPGTGSIVYSEANAASATNSAYNLTITDTGSPVTGTL
                     NLIGGQLGGILEFDDPSGVLMLGAGMFYAPSFSLDPITIANRVVTTTTSWTDQTNTDP
                     QAAGLTGIGPGVAQGTRVDTTTTINGTGSTNTITNAMYLPVGAKLGVIKDKLTLAGGY
                     TLTFNVTTTSTTTPAAVGPTTVTTVANTS"
     gene            complement(1940..2800)
                     /locus_tag="VFH83_02705"
     CDS             complement(1940..2800)
                     /locus_tag="VFH83_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET6485300.1"
                     /translation="MARLRLATCQFAVSGDLRVNARAILRQMREARRRGADLAHFSEA
                     CLSGYAGTDLPNLKGFDWDLHAELLADILRLAGELRLWVVVGSSHRLIPPHKPHNCLY
                     IISSAGRLVTRYDKRFCTASDQKHYTPGNAFTTCQVLGVRWGALICYDLRFPELYREY
                     YRLGVRLMLHSFHQAHGKPARKADGGNILGHITPASVFAHAANNYLWISANNSCARVS
                     RWGSFVAQPDGMLAGRLPLHRPGMILTTIDTTREFYDAPGKNRERAIKGLLSSLPPVR
                     DSRSRSRQTI"
     gene            complement(2810..3691)
                     /locus_tag="VFH83_02710"
     CDS             complement(2810..3691)
                     /locus_tag="VFH83_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6485301.1"
                     /translation="MKLCLFSISYAGLWGQERLELAEFIRKASDLGFDSVMIAGKRPH
                     LSPLDTDAARLGEVKARLQECRVDCSVIAAYTDFSGAGAAEVPYLEMQIAYVEALAGI
                     AASLGARVVRVFTAYESADLAPGLVWNRVVTSLRECCDRAAGRGLSIAIQNHHDLAVA
                     TDALLELLSDIDRPNCGLGFDAWSPALRGEDLYAAARKAAPHVLITTNADYVKLPRFH
                     YRPALINYQAAAPDLVRAVPFGEGFIDYAAFFSGLREGGFDGTATYEMCSPLRGGGSL
                     ANLDRCASRYLAWMRAP"
     gene            3821..>4633
                     /locus_tag="VFH83_02715"
     CDS             3821..>4633
                     /locus_tag="VFH83_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6485302.1"
                     /translation="MAYPVIMHVNYCEQGQEIEEICQKAARWGFDGVEFRRKRIGVAE
                     SMEDYLDRIEKGVKAAGLKHVLFGYPGPLLVKTDAAERRREVAEAISFYQLVARRFKV
                     KTVNLLTGTLQNPDKSFSYFDYTRHGSFLVTKEQWDWQVKGCQEMADGLKDVDIRFGF
                     ETHMVYVHDTVDAVRRLVAEIDRPSIGVNLDYGNIIDFQEHPSLEESIEALRGRIHYV
                     HLKNSAPLRGAPGRMGTALAEGEINNRQFVRMLVDAGYKGPICIEAPRAGDRE"
BASE COUNT          846 a         1396 c         1561 g          830 t
ORIGIN      
        1 cggaggtatt ggctacggtc gtgaccgtgg tgggtcccac cgcagccggc gtcgtggtcg
       61 aggtggtcgt gacattgaag gtgagcgtgt agccgcccgc gagggtcagc ttgtctttga
      121 tcacgcccag cttggcgccg accggcaggt acatcgcgtt cgtgatggtg ttcgtggagc
      181 cggtcccgtt gatcgtggtc gtagtgtcga cccgggtacc ttgggccacg cccggcccga
      241 taccggtgag gccggcggcc tgggggtccg tgttggtctg atcggtccag ctcgtcgtcg
      301 tcgtgaccac tcggttggcg atcgtgatgg gatccaggct gaaggaagga gcatagaaca
      361 tgccagcgcc cagcatcagg acgccggagg gatcgtcgaa ctcaaggatt ccgccaagct
      421 gtccaccgat gaggttcagc gtgccggtca cggggctacc ggtatcggtg atcgtcagat
      481 tgtaggcgga gttggtcgcc gacgctgcgt tggcctcact gtacacgatg gagccggttc
      541 cggggacctt gtagttctgg aagccgatcg tgtagctgac cgctccccgg gttctgaact
      601 tgagctggtc gcttaccttg agcaccggat cgatgtttcc gcccaaaccg accatgaacg
      661 tggagttcgc atagttcttg gtgtccagtg cgagagcacc gcccgtcagc gtccccaagt
      721 ccgcggtgac cgcagcgcgc aggttcgcct gggtcagcgt agtctggttc gggataaccc
      781 ccgccgaggt gtccggattg gtggtcccca ggccctcgtt gaaggtcagg gagctggtct
      841 tggctgcaac gcctgccgcg ttcacagtgg tgaagttgaa gctggccagg gggctgtagg
      901 cgaacgctac cgtgctatac ggctggccgc ccaccagaac cgtcgcggtc actggcatcg
      961 aaacgccgcc gaccgaaagg ggcatctctc cgttggcctg gaccgtccac gtcgtgccag
     1021 cgggaacggg ataggcggcg ggcttgccgt ccgcggttcc aaagaacacg ttcttggtgc
     1081 tcaccgtgtc cgcgggaaga ggggtacccg cgtccgtgcc ctgggttctc gcataggcgt
     1141 aggaaccccc gatgttgcgg gactgtccgt tcaggtacgc ccacagagaa acgccgagag
     1201 ggttgagatc gaaaccaccc gtcacgttga cggtagcgtt cgttgtgaga tcggtgtagc
     1261 tctcggtgtt ggtgaaggtg taatccggct ggttgttggc gttcgcgtcg accgtgccgg
     1321 tgttagacct cgtccagaag tcggtcagcc ctccgatatt cagaaggtcc gtctgtccga
     1381 gaacgaaacc gccggcggcg cccatacggt agctggccac cggcatggta aaaccaaaca
     1441 ggtatgtacc gatgttcgcg cctccaccct gctgcgtcgg cgctgtagtg ctgaagggat
     1501 tgacaactcc agttcccgtg aacggagaga ttccctggtt gaggatccag ttgtgctcaa
     1561 agttgccgta gctcgtgtag aggttgatcc ccttgtagtt ggagagctcg gctggcgagc
     1621 gaaagaggat gtccagctcg tcatagaaca ggccggacga cgcctgtgac agcatcgaac
     1681 ttggggcgga caacgtgaag ggattgatcc acgtcgtttc ctgcgcccag ccgatcgccg
     1741 caagggcgag accgatcaaa accagcaaaa ccagtctttt tctcatggtt ccctccttgg
     1801 acagaacctc gttatagaac atcctgttga ggcagatgca tctcaacatt ctcgaattta
     1861 gcacgtatct ctaaaatcac cacaagtttg ggaggcgatc ccacggaagg caggcgcgtc
     1921 tcggaggcta cccggcccgc tagatcgtct gcctggatcg agaccgactg tcgcggacgg
     1981 gtgggaggga agagagcaga cccttgatgg ctcgctcccg gttcttgccg ggggcgtcgt
     2041 agaactcccg agtcgtgtcg atcgtggtga ggatcatgcc agggcgatgg agcggcaaac
     2101 ggccggcgag catcccgtcc ggctgcgcga cgaagctgcc ccaccgagag acccgcgcgc
     2161 agctattgtt ggcgctgatc cacaggtagt tgttggccgc gtgggcgaat acgctcgccg
     2221 gagtgatatg cccgaggatg ttgccgccat ccgctttccg agccggcttg ccatgagcct
     2281 ggtggaagga gtggagcatc agtcgaaccc ccaggcggta gtactcccgg tagagctcgg
     2341 ggaaacgcaa gtcgtagcag atcagggctc cccagcgcac cccaagcacc tggcaggtcg
     2401 taaaggcgtt gcccggcgtg tagtgcttct ggtcgctggc ggtgcagaag cgcttgtcgt
     2461 accgcgtcac cagccggccc gcggagctga taatatagag gcaattgtgc ggcttgtgag
     2521 gagggatcag ccggtggctg gacccgacga ccacccagag gcgcaactct ccagccaacc
     2581 gcaggatgtc cgccaggagc tcggcgtgga ggtcccagtc gaaccccttc aggttcggaa
     2641 gatccgttcc tgcatagccg gagagacacg cttcgctgaa atgcgccagg tcggcgccgc
     2701 gccgacgagc ctcgcgcatc tgccgcagga tggcgcgggc gttgactcgg agatccccgc
     2761 tgaccgcgaa ctggcacgtg gccagcctga gacgcgccac gcggatttcc tagggggccc
     2821 gcatccaggc caggtaccgg gaggcgcatc ggtccaggtt tgccaggctc ccgcctcctc
     2881 ttagcggcga gcacatctcg taggttgcgg ttccatcgaa tccgccttcc cgcaggccgg
     2941 agaagaacgc ggcgtaatcg atgaacccct ccccgaacgg aacggcccgc accaggtccg
     3001 gcgccgcggc ctggtagttg atcagagcgg gccgatagtg gaacctcggc agcttcacgt
     3061 aatccgcgtt ggtggtgatg aggacgtgcg gcgccgcctt tcgggccgcg gcatagaggt
     3121 cttccccgcg aagcgccggg gaccaggcgt cgaaccccag tccgcagttg gggcggtcga
     3181 tgtcgctcag gagctccagg agggcgtcgg ttgccactgc caggtcgtgg tggttctgga
     3241 tggcgatgga aaggccgcgg ccggccgcgc ggtcacagca ctcccgcagg gaggtcacga
     3301 cccggttcca caccaggccc ggcgccaggt ccgcgctctc atacgcggtg aacacgcgca
     3361 ctacgcgcgc gcccaggctg gccgcaatcc cggcaagggc ttccacatag gcgatctgca
     3421 tctccaggta cggcacctcg gcagctcccg cgccggagaa gtccgtgtac gccgcaatca
     3481 cggagcaatc gactcggcac tcctgcagcc gcgccttgac ctcccccagg cgcgccgcat
     3541 cggtgtccag cggcgaaaga tggggacgct tgcccgcgat catcacggaa tcgaagccca
     3601 ggtcggaggc cttgcggatg aactccgcga gctccagccg ctcctggccc cagaggcctg
     3661 cgtagctgat cgagaagagg cacagcttca tgagagtggg agtctgcgcc cccgccagcg
     3721 ccctgtcaac gagcggggct ttgggggccg cttgccgcgg agaggagctt gagcgaaacc
     3781 gggcccggcg gtacaacgct gggtaccgag aggagacgcg atggcatacc cggtgatcat
     3841 gcacgtgaac tactgcgagc aggggcagga gatcgaggag atctgccaga aagcggcccg
     3901 atgggggttc gacggagtcg agttccgccg aaagcgaatc ggagtcgcgg agtccatgga
     3961 agattacctc gaccgcatcg agaagggagt gaaggccgcc ggcctgaaac acgtgctctt
     4021 cgggtatccc ggaccgctgc ttgtgaagac ggatgccgcg gagcgccggc gcgaggtcgc
     4081 ggaggccatc tccttctacc agctcgtggc tcggcggttc aaggtgaaaa cggtgaatct
     4141 cctcaccggc accctccaga acccggacaa gagcttctcc tatttcgact acacccgcca
     4201 cggatccttc ctggtcacca aggagcagtg ggactggcag gtgaagggtt gtcaggagat
     4261 ggccgacggg ctgaaggacg tcgacatccg gttcgggttc gagacacaca tggtgtacgt
     4321 gcatgacacg gtggacgcgg tgcgccgcct ggtggcggag atcgatcggc cgtcgatcgg
     4381 cgtcaacctg gactatggca acatcatcga ttttcaggag cacccgtccc tggaggaatc
     4441 catcgaggcg ctgcgtggtc ggatccatta cgtccacctc aagaactccg ctccgctgcg
     4501 cggggcgccc ggccgcatgg gcacggcgct ggccgaggga gagatcaaca accgccagtt
     4561 cgtgcggatg cttgtggacg ccgggtacaa gggtcccatc tgcatcgagg cgccgcgagc
     4621 gggtgaccgg gaa
//
LOCUS       DASRZS010000114         7803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3909417, whole genome shotgun sequence.
ACCESSION   DASRZS010000114 DASRZS010000000
VERSION     DASRZS010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7803
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3909417"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /gene="rpsM"
                     /locus_tag="VFH83_02720"
     CDS             <1..286
                     /gene="rpsM"
                     /locus_tag="VFH83_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET6485303.1"
                     /translation="ARKICATTGVDPEKRTNDLGPDEVNKLRTVIESDYRVEGRLRTE
                     VSLNIKRLMDIGCYRGIRHRRGLPVRGQRTKTNARTRKGKRKTVAGKRKK"
     gene            399..782
                     /gene="rpsK"
                     /locus_tag="VFH83_02725"
     CDS             399..782
                     /gene="rpsK"
                     /locus_tag="VFH83_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET6485304.1"
                     /translation="MAKTKGKKEKKKNIAEGKAFIQATFNNTIVTITDLMGNTISWAS
                     AGGLGFKGAKKSTPFAAQTTAESAAQKAMEQGLRELVVFVKGPGVGSEAAIRAFGVLG
                     LRVRSIKDTTPIPHNGCRPPKKRRV"
     gene            795..1430
                     /gene="rpsD"
                     /locus_tag="VFH83_02730"
     CDS             795..1430
                     /gene="rpsD"
                     /locus_tag="VFH83_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET6485305.1"
                     /translation="MARNIGPQCRLCRSHGYKLYLKGDRCKGPKCPVTRKKGAPGKGP
                     RSRTKKISDYGVQLHEKQKLKRMYGMLEGQFRQFFSRAVSKRGITGEYLIQMLERRLD
                     NVVYRMHLAPSRKSARQLVLHGHISLNGRRASIPSLLVRENDQIAVREPSTKLVAIKE
                     SLKEYSRSGIVPWLEIDPDRMAGKVRAIPRRSEITDLSDIREQLIVELYSK"
     gene            1441..2589
                     /locus_tag="VFH83_02735"
     CDS             1441..2589
                     /locus_tag="VFH83_02735"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET6485306.1"
                     /translation="MVRKNLLRGFKKPKGITFEHSEVEANYGRFVAYPFERGYGVTIG
                     NTLRRILLSSIQGYAVSAVKVTTYKDDGSPHMIASEYEPIPEVVEDTPDFINNLKHVQ
                     LRLNGDLEEKTILVEAKGARTLTAKDLAVDTEVEVLNGDLVLATLMEKANLEFEVQIN
                     LGRGYVPAERNEKYIEVIGTVPVDAIFSPIQKVKYSVENTRVGQRTDYDKLILEVWTN
                     GTITPDDALAEAAKIAKDHFTIFINFDEAEAGGDDEIDEEEERVRALLDTPVEELELS
                     VRSSNCLRNANIRTIGDLTKKTEEEITKTRNFGKKSLQEIRDKLQARGLDLGMKDYSA
                     LKKQLKRQLAAESAAPAAVAAPAAVVSAGDADDDPVEEPAQGKGIDTP"
     gene            2586..2996
                     /gene="rplQ"
                     /locus_tag="VFH83_02740"
     CDS             2586..2996
                     /gene="rplQ"
                     /locus_tag="VFH83_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET6485307.1"
                     /translation="MNHRSSINRLGRTSAHRKALLKSQAKALFRYERIRTTMPKAKEA
                     RRLAEKLITRAKVDSVHNRRIASRSIDDGGVLNKLFTEIAPRYAQRPGGYTRILKMGK
                     RFGDASEMVILELLDRKERERLRKKKPAEAAKEG"
     gene            3112..6687
                     /locus_tag="VFH83_02745"
     CDS             3112..6687
                     /locus_tag="VFH83_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485308.1"
                     /translation="MRQSLKIAISLLASLLLFAGFAVVAFAGLFDFVQASFFLPRVQE
                     GYREEVLTLGTRIEAFHQANLERFAVVAGKDFAQATLGTSLPPAAVEQWASTLSTLNV
                     LSVRVLSADGSRILFSSLQGDVRSQDATRIAYRNYNEVGDSIAADRLILRPGEAPRVL
                     VDGDHGRFLYVLPVTRPATPAGPEDRGTAVFAVGAVDLLNDLQLTAQVPVTRVSLVGA
                     DGVLLNFPVAEPGPLNARLESAWRGHPGEASFTDAINLTGADGATQGFTIFSLRLKQG
                     GTASLVVMASAFAMSDLMKGLLLATFFCTVFLIIYLLLNLRSDPLEVLRQRVKRFQIQ
                     LIGEMVDRPGGPEWGRLRGEMEARRDEITWHIQRGIGRISRKQKPVIDEYLNKSWTEI
                     LELISRRAEQPRPVAAGSVDVARLEALIQAALQNAHFMLPPQRGPAAVVREAAPEARP
                     AAPQGVATVVAEATAEAEEVQEAEEVQEAESVDEAEPVGEAEPVDEAEPVGEAGPVDE
                     AGPVDKAESVEEAEPIDEAEPVEEAEPIDEAEPVGEAQEAEDAEPAEAIEDAEPVEAI
                     EDAEPVEAVAEAEPVAEAETAEEGDAAELAEPAVDLDTPAEPGAVRELERAPDAEPAE
                     EVEEAETVEEAEEAEAAEIEIEAEPGPVLGSPPGAEPFRGSSDAEREIINADDIVEDL
                     ESAPEDDSDDSAEEPEIIDADAIEEAELIEEAPAQSESSGVGAGARGTTGAKPGSDAW
                     AARLQSSDLLHFESSARGAAAGADGQPSVADLSMDLPIDSPHEVLEELESVAEITPLP
                     PEPFEEGLELLPIVEETEGSAGASAKGDAMPGGAKGASGKDETEGPAKAAEGQQRPTP
                     ASKRPAEPPVEAKPAASPPRQPPAQRAKVGRGREYEVEELEVIDESESESPQMSEAEQ
                     REELEKLTSSGVLKSWSLEELRTVVEANRSAVVMEDGVYRIKKELYAPSEAEGQTTPG
                     SRALREAADQAIRGPGDSPTVPAGGAPGESGGIRDLIHDEDTFDLSKVITPEREGPAE
                     SRAGAKEQTIPLRLGRAGIDYDDFLSLYPRSLSHTTLMKSLVEVSRRVSAVAAVLFLK
                     KPEGYLPDITLGVTEETARKLRFGVAEPFTQRLLTPRRTLALGQGVALTRCLGPKVDP
                     EDHRYMKRLIMVPATFRAQDGYLLLSFALESVSSIEKLLAKLLVH"
     gene            6805..>7803
                     /locus_tag="VFH83_02750"
     CDS             6805..>7803
                     /locus_tag="VFH83_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HET6485309.1"
                     /translation="MSTLLYIILPLAGVLLGWLIRWLYARFQLSSAEQKASHVVGEAL
                     REAEAKKKEAILESKDQLLRERNQLEKETRERRIELQRVERRLLQKEENLEKRVETLE
                     KQERSVGNREREAADREAALEQERQRIGTELERVSGLTRAEAKKLLVQSLENEARHDA
                     QVTINLVEQEAKLTADKKARDILITTIQRLATDVASEATVTTVSLPNDEMKGRIIGRE
                     GRNIRTLETLTGVDIIIDDTPEAVVISCFDPIRREIAKVALSRLISDGRIHPARIEEI
                     VQKVTKEVNQTIYEEGERVLFEVGIHSMGPEAIRNLGRLHYRTSYGQNVLAHSKEVT"
BASE COUNT         1579 a         2408 c         2686 g         1130 t
ORIGIN      
        1 cgcgcgcaag atctgcgcca cgacgggcgt ggacccggag aagcggacca acgacctggg
       61 tccggacgag gtgaacaagc ttcggaccgt gatcgagagc gactaccggg tggaggggcg
      121 ccttcgaacc gaggtctctc tcaacatcaa gcggctgatg gatatcggct gctaccgggg
      181 gatccgccac cgccgcggac tgcccgttcg cggacagcgg accaagacca acgcccgcac
      241 ccgcaagggc aagcggaaga cggttgccgg gaagaggaag aagtagagcg agggcgccgc
      301 gtcccaggac ggacgaaagc ggcgccggcg ttgacattgc ggtgcgtcgg ggcgggcctc
      361 gacccgccgg tgggtgtctc tgttaggagg agtcgacttt ggcaaagacc aagggcaaga
      421 aggaaaaaaa gaagaatatc gcggaaggga aggcattcat ccaggcgacc ttcaacaaca
      481 ccatcgtgac catcaccgac ctcatgggca acacgatttc ctgggcgtcc gcgggaggcc
      541 tggggttcaa gggcgccaag aagtccaccc cgttcgccgc gcagaccacg gcggagtcgg
      601 cggcccagaa ggccatggag cagggtctgc gggagctggt ggtgttcgtc aaaggcccgg
      661 gggtgggaag cgaagccgcc atccgcgcct tcggggtcct gggcctgcgg gtgcggtcca
      721 tcaaggatac cacccccatt ccccataacg ggtgccgtcc gccgaagaag cggcgcgtgt
      781 agggaggcga gacgttggca aggaatatcg gtccgcagtg ccggctgtgc cggagccacg
      841 gctacaagct ctacctcaag ggtgacaggt gcaaggggcc caagtgcccc gtgacgcgca
      901 agaagggggc gcccggcaag gggccccgca gccgcaccaa gaagatctcc gattacgggg
      961 tccagctcca cgagaagcag aagctgaaga ggatgtacgg gatgctggaa ggccagttcc
     1021 gccagttctt cagcagagcc gtgagcaagc gcgggatcac cggtgagtat ctcatccaga
     1081 tgctggagcg ccggctggac aacgtggtct accgcatgca cctggcgccg tcgcgcaaga
     1141 gtgcgcgcca gctcgtcctg cacggccaca tctccctgaa cggccgccgc gcgtccatcc
     1201 cgtccctcct ggtgcgggag aacgaccaga tcgcggtccg cgagcccagc accaagctgg
     1261 tggccatcaa ggagagcctg aaggagtact cgcgctcggg catcgtgccg tggctggaga
     1321 tcgatccgga tcgcatggcg ggcaaggttc gggccatccc gcgccgcagc gagatcaccg
     1381 acctgtcgga tattcgggaa cagctgatcg tcgagctgta ttccaagtga ggtgctccct
     1441 atggtccgaa agaacctgct gaggggtttc aagaagccca agggcatcac cttcgagcac
     1501 agcgaggtag aggccaacta cggccgcttc gtggcctacc cgttcgagcg aggctacggg
     1561 gtcaccatcg gcaacacctt gcggcggatc ctgctgtcct ccatccaggg gtacgcggtc
     1621 agcgccgtca aggtgaccac ctacaaggac gatgggtcgc cccacatgat cgccagcgag
     1681 tacgagccca tccccgaggt ggtggaggac acgcccgatt tcatcaacaa cctcaagcac
     1741 gtccagcttc ggctgaacgg ggacctggag gagaagacca tcctggtgga ggccaagggc
     1801 gcccggaccc ttaccgccaa ggacctggcc gtggacaccg aggtggaggt cctcaacggc
     1861 gacctcgtgc tggccacgct catggaaaag gccaacctcg agttcgaggt ccagatcaac
     1921 ctggggcgcg gatacgtgcc ggcggagcgc aacgagaagt acatcgaggt catcgggacc
     1981 gtcccggtgg acgccatctt ctcgcccatc cagaaggtca agtacagcgt cgaaaacacg
     2041 cgcgtgggac agcgcacgga ctacgacaag ctcatcctgg aggtgtggac caacgggacc
     2101 atcaccccgg acgacgccct ggcggaggcc gccaagatcg ccaaagacca cttcacgatc
     2161 ttcatcaact tcgacgaggc ggaagccggt ggagacgatg agatcgacga ggaggaggaa
     2221 cgggtgcggg ccctcctgga cacgccggtg gaggagctgg agctctccgt gcggtccagc
     2281 aactgcctgc gcaacgccaa catccggacc atcggggatc tcaccaagaa gacggaagag
     2341 gagatcacca agacccggaa cttcggcaag aagagcctcc aggagatccg ggacaagctc
     2401 caggcgcgcg ggctggacct gggcatgaag gactactccg cgctgaagaa gcagctcaag
     2461 cggcagcttg ccgcggagtc cgccgccccg gccgccgtgg ccgcgccggc cgccgtggta
     2521 tccgcgggtg acgccgacga cgacccggtg gaggagccgg cccaagggaa gggaatcgat
     2581 acgccatgaa ccatcgaagc agcatcaacc gcctgggacg gacctcggcg caccgcaagg
     2641 cactgctcaa gtcgcaggcc aaggccctgt tccgctatga gcgcatccgc accaccatgc
     2701 ccaaggccaa agaggcccgc cgtctggcgg agaagctcat cacccgggcc aaggtggact
     2761 cggtccacaa ccgccggatc gcctcgcggt ccatcgatga cggcggggtg ctgaacaagc
     2821 tgttcacgga gatcgctccg cgctacgccc agagacccgg cggctatacg cggatcctca
     2881 agatgggcaa gcggtttggg gacgcctcgg agatggtcat cctggagctg ctggaccgca
     2941 aggagcggga gcgtcttcgc aagaagaagc cggcggaagc ggccaaggaa ggctgacacc
     3001 cgcgtccgtc ccgctccgtc cgctcgccga cgatggcccc ccgtgcgccg gggggcttgt
     3061 tgttcgcgca gaattgcgcc gataatcggg ataaggtaaa tccagttcct catgcggcag
     3121 agcctgaaaa tagcgatcag tctcctcgcc tccctcctgc tctttgcggg ttttgccgtt
     3181 gtggctttcg cgggtctttt cgatttcgtc caggcgagct ttttcctgcc cagggtgcag
     3241 gagggctacc gcgaggaagt cctgaccctg ggcacccgga tcgaggcgtt ccaccaggca
     3301 aacctggagc ggttcgcggt ggtggcgggc aaagacttcg cccaggccac gctcggcacc
     3361 tctctcccgc ctgccgcggt cgagcaatgg gcgtccaccc tgtccacgct gaacgtcctc
     3421 tccgtccggg tcctgagcgc ggacggcagc aggattcttt tcagctctct gcaaggggat
     3481 gtgcgatccc aggacgcgac gcggatcgcc tatcgcaact acaatgaggt cggcgactcc
     3541 attgcggcgg accgcctcat cctgaggccg ggggaggccc cccgggtcct cgtggacggc
     3601 gaccacgggc gctttctcta cgtgctgccg gtgacccgcc cggcaacgcc ggccgggccg
     3661 gaggatcggg gcacggcggt gttcgccgtg ggggcggtcg acctcctcaa cgacttgcag
     3721 ctcaccgctc aggtcccggt gacccgggtg agcctggtcg gcgccgacgg ggtcctcctg
     3781 aacttccccg tggcggagcc cggacccctc aacgcccggc tcgagtccgc gtggcggggt
     3841 caccccggcg aggcttcctt caccgacgcc atcaacctga cgggggcgga cggcgccacg
     3901 cagggcttca cgatcttctc cctgcgcctg aagcagggcg gcacggcgag cctggtggtg
     3961 atggcctccg ccttcgccat gagcgacctg atgaaggggc ttctcctggc caccttcttc
     4021 tgtaccgtat tcctcatcat ctaccttctc ctcaacctga ggtcggaccc cctggaggtg
     4081 ctgcgtcagc gggtcaagcg attccagatc cagctcatcg gggagatggt ggaccgcccc
     4141 ggcggtcccg agtggggcag gctgcgcggc gagatggagg cccggcggga cgagatcacc
     4201 tggcacatcc agcgcgggat cggaaggatt tcccgcaagc agaagccagt gatcgacgag
     4261 tacctcaaca agagctggac cgagatcctg gagctcatct cgaggcgtgc cgagcagccg
     4321 aggcccgttg cggcgggctc cgtggatgtc gcccgtctcg aggcgctcat ccaggccgcg
     4381 ctccagaacg cccacttcat gcttccgcct cagcgcggtc cggctgccgt tgtccgtgag
     4441 gcggcaccgg aagctcggcc tgccgcgccg cagggcgtgg ccaccgtggt agcagaagcc
     4501 actgcggagg ccgaggaggt ccaggaggcc gaggaggtcc aagaggccga gtcggtcgat
     4561 gaagccgagc ccgtcggtga ggctgagccg gttgatgaag ctgagccggt tggtgaagct
     4621 gggccggttg atgaagcagg gccggtcgat aaagctgaat cggtggaaga ggctgaaccg
     4681 atcgatgaag ctgagcccgt ggaagaggct gaaccgatcg atgaagctga gcccgttggt
     4741 gaagcccagg aagctgagga tgcggagccc gcggaagcga tcgaggatgc ggagcccgtg
     4801 gaagcgatcg aggatgcgga gcccgtggaa gcggtagccg aggcggaacc agtcgcggag
     4861 gccgagaccg cagaagaggg cgatgcggcc gaactggcag agcccgcggt cgatctggat
     4921 accccggcgg agccgggggc ggtccgggag ctcgagcgcg ctcccgacgc ggaacccgcc
     4981 gaggaggtcg aggaggccga gacggtagaa gaggccgagg aggccgaagc cgcggagatc
     5041 gagatagagg cggaaccagg gcctgtgctc gggtccccgc cgggggcgga gcctttccgc
     5101 gggtcttccg atgccgagcg cgagatcatc aacgcggatg atatcgtcga ggatctggag
     5161 tcggcacccg aggatgacag cgacgactct gccgaggaac ccgagatcat cgacgccgac
     5221 gccatagagg aggccgagct catcgaggaa gcgccggctc agagtgaatc ctctggggtc
     5281 ggcgccggcg cccgaggaac aaccggagcc aagccgggct ccgacgcctg ggccgcgcgc
     5341 cttcagtcca gcgatctcct ccatttcgag agctcggccc ggggggcagc cgccggagcg
     5401 gatggccaac cctcggtggc ggacctgtcc atggatcttc ccatcgactc gcctcacgag
     5461 gtgctggagg agctggagtc cgtggccgag atcacgcctc ttccaccgga gccgttcgag
     5521 gagggcctgg agctgctgcc gatcgtcgaa gagaccgaag ggtcggccgg ggcttcggcc
     5581 aagggggatg cgatgccagg gggagccaag ggcgcgtcgg gcaaggatga gacggagggg
     5641 cccgcgaagg cagccgaggg gcagcagcga ccaactcctg catcgaagcg accggcagag
     5701 ccgccggtgg aggcaaaacc ggcggcgtcg ccgccgcggc agccgcccgc acaacgggcc
     5761 aaggtcgggc ggggacggga atacgaggtg gaggagcttg aggtgatcga cgagtcggaa
     5821 tccgagtccc cccagatgtc cgaggcagag cagcgggagg agctggagaa gcttacctcc
     5881 agcggcgtgc tcaagagctg gagtctggaa gagcttcgga ccgtcgtaga ggcaaaccgt
     5941 tccgccgttg tcatggagga cggcgtctat cggatcaaga aagagttgta tgccccttcg
     6001 gaggctgagg gccagaccac gccgggctca cgggcgctgc gggaagccgc cgatcaagcc
     6061 atccgcggtc ccggggattc accaaccgtc cccgcggggg gcgcccccgg agagtccggc
     6121 gggatccgcg acctgatcca tgacgaggac acgttcgacc tgtcgaaggt catcacgccc
     6181 gagcgggagg ggccggcgga gtcccgggcc ggcgccaagg agcagaccat tccgttgagg
     6241 ctcggccgcg ccggaatcga ctacgacgat ttcctctccc tttacccgcg ctccttgagc
     6301 catacgaccc tcatgaagtc cctggtggaa gtgtcgcgtc gagtctccgc cgtggccgcc
     6361 gtgctgttcc tcaagaaacc cgaggggtac ctgccggaca tcaccctggg cgtgaccgag
     6421 gagaccgcgc ggaagctccg attcggcgtt gcagagccct tcacccagcg cctgctcacg
     6481 ccgcggagga cgctggccct gggacagggg gtcgccctga cccggtgcct gggcccgaag
     6541 gtggatcccg aggaccatcg gtacatgaag cggttgatca tggtgccggc gaccttccgg
     6601 gcccaggacg gatacctgct cctgtcgttt gcgctggaaa gcgtgtccag catcgagaag
     6661 ctcctggcga aactactggt tcactagctc cgttccgagg gaatgacata ttctcaccgt
     6721 gttatgaagg gagctctccc ttgacaaagc ccggcttcta gcctacgcta taaaagtatc
     6781 tatattacca ggtggaggaa gagtatgagc acattgttgt acatcatact ccctctcgcc
     6841 ggagtgcttc taggatggct aattcggtgg ctttacgcgc gatttcagct ttcatcggca
     6901 gaacagaagg cttcccacgt cgtaggggag gccctcagag aagcggaagc gaaaaagaaa
     6961 gaggcgatac ttgagagcaa ggaccagttg ctgcgggaac gcaaccaact ggaaaaggag
     7021 acgcgggagc ggcggatcga gctccagcga gtggagaggc gcctccttca gaaggaagag
     7081 aacctagaga agcgcgtaga aaccctcgag aagcaagaga gaagcgtagg gaaccgggag
     7141 agggaagccg cggaccgaga agcggcgctc gagcaggaac gacaacggat cggaaccgag
     7201 ctcgagagag tatcggggct taccagggcg gaagcgaaga agctcctggt gcagagcctg
     7261 gaaaacgagg cccgtcacga cgcgcaggtc acgatcaacc tcgttgaaca ggaagccaag
     7321 ctaaccgcgg acaagaaggc ccgcgacata ctgatcacga ccatccagcg cctggccacg
     7381 gacgttgcct ccgaggccac ggtgaccacc gtcagcctgc ccaacgacga gatgaagggc
     7441 aggatcatcg ggagggaagg ccgcaacatc cggaccctgg agacactcac cggggtagac
     7501 atcatcatcg acgacacgcc ggaggcggtc gtcatttcct gtttcgatcc catccgtcgg
     7561 gagatcgcca aggtggcgct gagccggctg atctcggacg gaaggatcca cccggcccgc
     7621 atcgaagaga tcgtgcagaa ggttaccaaa gaggtgaacc agacgatcta cgaggagggc
     7681 gaacgggtcc tgttcgaggt ggggatccac tcgatgggac cggaggccat ccgcaacctc
     7741 gggcggctgc actaccggac cagctacgga cagaacgtgc tggcgcattc caaggaagtg
     7801 acg
//
LOCUS       DASRZS010000115         2957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1215412, whole genome shotgun sequence.
ACCESSION   DASRZS010000115 DASRZS010000000
VERSION     DASRZS010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215412"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFH83_02755"
     CDS             <1..204
                     /locus_tag="VFH83_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HET6485310.1"
                     /translation="VVALIKEEGPGRFSVGLRSTSSVDVGNIAAGFGGGGHRQAAGFD
                     IAGTLASVRQTLLDVFAPILSKP"
     gene            319..1167
                     /locus_tag="VFH83_02760"
     CDS             319..1167
                     /locus_tag="VFH83_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485311.1"
                     /translation="MESLTNKVLLFQKTGQGLQEIVAAVSMRVYQFPRWKLGWDEDAC
                     GEFYLFFHPRLLRLVGRFRDQGKPFESYLASVLAWQLRNFARGRGRAERSWSVALRLQ
                     AEDPDGERSEPPRDTVLVPAAIRGVIRSDADRRNLVYLVLKCSRTLDEATMSALAELA
                     RFDLDRLQCLAEQLRREREPRDRRLETFRLRRNRAFSLARLLETELQGETDPERIAAL
                     RCRVSSARRRMEAAMKRMSRVGLAPTNREIARVLGVPKGTVDSGLYWLKRKLSPVYDP
                     DELRLA"
     gene            1164..1799
                     /locus_tag="VFH83_02765"
     CDS             1164..1799
                     /locus_tag="VFH83_02765"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HET6485312.1"
                     /translation="MMEQLLLASNNEHKHREFIRLFPGVSVVLPRELGLDFDYQEGEE
                     SYLVNAMGKGMSLFRQAQGQGGNGPLPVIADDSGLSVPALRGEPGIRSSRYGSKPGQP
                     VLEAPQRNLYLLDRMDGIQDRRAFFVCCLVLVLGENRYIIVQETVDGTITEAPRGANG
                     FGYDPLFLFPHSGKTMAELTDDEKDRISHRGRAARRMRALAASEGGTTGWT"
     gene            1790..2842
                     /locus_tag="VFH83_02770"
     CDS             1790..2842
                     /locus_tag="VFH83_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET6485313.1"
                     /translation="MDLISTYANGVSELTNFSQSVERGPLMVDVIVNPHAGFFKRRAT
                     VWKHVQELELKLTELRKRYPRRKVEIHTVHFTERPGHARTITEQILQEEEKTQSGIEH
                     LLIGCGGDGTSNEICTALVQASGSLLDRLKLLRLPLGTGNDMADAATFSEAYDLILGS
                     QQAAQTGALVIRTACGGEHYSFNIASIGLDAYIADLTNRFKRVIPGDAYKSLVDLGSL
                     FYDQRVKLQPMDLLLHSTAGASKIDAFLPSMVIVGVSGRRTYGGHIPALPGPQNVCVV
                     GHMSVLKKVRSKKVFYEGRHHELAEVSFHSAQSVEIGYPSRIPMQVDGEVSWLGPQDF
                     PVRMQILDPKIKILRS"
BASE COUNT          503 a          941 c          986 g          527 t
ORIGIN      
        1 gtggtggcgc tgatcaagga agaggggccc ggccggttct ccgtgggtct ccgttcgacc
       61 tcctcggttg acgttgggaa catcgccgcg gggttcggcg gcgggggtca tcggcaggcc
      121 gccgggttcg acatcgccgg gacgttggcc tccgttaggc agacccttct ggatgtcttc
      181 gcccccatcc tctcgaagcc ttgatctgcc gtccccgttc tccagccatt tgccgattgt
      241 aagcgaacgt aaagtctcct gttccccgaa gccggtgccc catggtctcc tggcacgccc
      301 gttgctaccc gggaggacat ggaaagtctc acgaacaaag tcctcctctt ccagaagacg
      361 ggacagggac tgcaggagat cgtcgcggcc gtgtcgatgc gggtctacca gtttccgcgt
      421 tggaagctgg gctgggacga ggatgcctgt ggggagttct atctcttttt ccatcccagg
      481 ctgcttcggc tggtcggccg atttcgggac cagggcaagc cgttcgagtc ctatctcgcc
      541 tcggtgctgg cctggcagct acggaacttc gctcgcggcc gtgggcgcgc ggagcggtcc
      601 tggagcgtgg cgctccggct tcaggccgaa gatcccgacg gtgagaggtc cgagccaccc
      661 cgggatacgg tccttgttcc cgctgccatc cgcggcgtga tccgtagcga cgccgatcgg
      721 cggaatctcg tctatctcgt cctgaagtgc tcccgaacct tggatgaggc caccatgagc
      781 gcgctcgcgg agctggcgcg cttcgacctg gatcggctcc agtgcctcgc ggagcagctc
      841 cgccgggagc gcgagccccg ggaccgtcgg ctggagacgt tcaggctgcg aaggaaccgc
      901 gccttctcgc tggcccggct cctggagacc gagctgcaag gtgagacgga cccggaacgc
      961 atcgccgctc tccgctgtcg ggtgtccagc gcgcgtcgac gcatggaggc ggccatgaag
     1021 cggatgtccc gcgtcggcct ggcgccaacc aatcgcgaga tcgcccgggt gctcggggtc
     1081 cccaagggga ccgtggattc cgggctttac tggttgaaga ggaaactgag cccggtatat
     1141 gatcccgacg agctcaggct ggcatgatgg aacagcttct cctggcctcc aacaacgagc
     1201 acaaacaccg ggagttcatc aggctctttc cgggagtctc ggtcgtgctg ccccgggagc
     1261 tcgggctgga cttcgactat caggaagggg aagagagcta tctggtcaac gccatgggca
     1321 aggggatgtc cctctttcgg caggcccaag ggcagggggg caacgggccc cttcccgtga
     1381 tcgcggacga ctccggcctg agcgtacccg cgctgcgcgg agagccgggc atccgttcct
     1441 cccgatacgg gtcaaagcca ggacagccgg tgctggaggc tccccagcgc aacctctatc
     1501 tcctggaccg gatggacggg atccaggacc gccgcgcgtt tttcgtctgc tgcctggtcc
     1561 tggtgttggg cgagaatcgc tatatcattg tccaggagac cgtggacggg accatcaccg
     1621 aggcgccgcg cggcgccaac ggttttggat acgatcctct tttcctgttc cctcattcgg
     1681 gcaaaacgat ggccgagctc accgatgacg agaaagaccg catctctcac cgggggcggg
     1741 cggcgcgccg gatgcgggct ctggcggcct cggaaggcgg aacaaccgga tggacctgat
     1801 cagcacgtac gcgaacggcg tctcggagct caccaacttc tcccagtcgg tcgagcgggg
     1861 cccgttgatg gtggacgtga tcgtcaatcc tcacgcgggg ttcttcaagc ggcgggccac
     1921 ggtctggaag cacgtccagg agctggagct gaagctgacc gagctgcgca agcgctatcc
     1981 tcgtcggaag gtcgagatcc acacggtgca cttcacggag cggccggggc acgccagaac
     2041 catcacggag cagatcctgc aggaggaaga gaagacgcag tcggggatcg agcacctcct
     2101 gatcgggtgc ggcggcgatg ggacgagcaa cgagatctgc acggctctgg tccaggcgag
     2161 cggctccctt ctggatcggc tgaagctgct tcggcttccc ctgggcaccg gcaacgacat
     2221 ggcggacgcg gccaccttct cggaggccta cgacctcatc ctgggttccc agcaggcggc
     2281 ccagaccggc gccctggtga tccgcacggc ttgcggcgga gagcattact ccttcaacat
     2341 cgccagtatc gggctggatg cgtacatcgc ggacctgacc aaccggttca agcgggtgat
     2401 cccgggggac gcctacaagt ccctggtgga cctgggctcc ctgttctacg atcagcgcgt
     2461 gaagctgcaa cccatggacc tgctgctcca ctctacggcg ggagcatcga aaatcgacgc
     2521 cttcctgccg tccatggtga tcgtcggcgt cagcggtcgg cgcacctacg gcggccacat
     2581 tccggcgctg ccggggccgc agaacgtctg cgttgtgggg cacatgagcg tgctcaagaa
     2641 ggtccgcagc aagaaagtgt tctatgaggg ccggcaccac gagcttgccg aggtcagctt
     2701 ccacagcgcg cagagcgtcg agatcggcta tccttcccgg attcccatgc aggtcgatgg
     2761 tgaggtatcc tggctcgggc cccaggactt ccccgttcgg atgcagatcc tggaccccaa
     2821 gatcaagatc ctgagaagct aaccatggca caaccgaaac cggtatccac ggcgggggcg
     2881 gtccgcccct cggcccttgg acgcgagact gcccttggcg gcgggggcgc gaagctgagc
     2941 gatgcagacc gtcgtcg
//
LOCUS       DASRZS010000116         5539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2754976, whole genome shotgun sequence.
ACCESSION   DASRZS010000116 DASRZS010000000
VERSION     DASRZS010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2754976"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2443)
                     /locus_tag="VFH83_02775"
     CDS             complement(<1..2443)
                     /locus_tag="VFH83_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6485314.1"
                     /translation="MSVEQGFDAPVSSPIPDARRLQSILDSLLEGCAIFDRDWKCFYA
                     NETAARLIGGPRDSLVGLSIMEAFPRIETTKVIQLLRRCMEEAAPARVEAELPLRSGP
                     PQWFDLSCSPVPEGVLLLLCDITERKVGEESLRANEARFRSLVEQAGEAMFVHDFSGR
                     FVEVNPQACRSLGYSRDELLSMTVMDVEQDFDLPAAQREWAKIELERPFTLYGHQRRK
                     DGTTFPVEVRFGRFDSGGQRFFLGMARDITDRREAELRQKHLTEVLRAIRSVNQLIVH
                     EKDPKALLQEACDLLTATRGYESAWVATRDSGGCLHAVAEARLSDTEAFRLMQAGMDR
                     GMVPPCCSQAAARPDGIVLHDLSTDCAGCILAKGYQGMAALVAALRASGRDYGVLVAA
                     LPLALARDEEEQSLFREITEDIGFALQSIESERERLRAVEALSKSEGRLKAALSSMTD
                     AVSLADPQGTFTDFNEAFATFHRFRGKQECARTLAEYPGFLDVFLPNGEPAPLDQWAV
                     PRALRGETVANAEYRLRRKDTGETWIGSYSFAPIRDRSGAIVGCVVVGRDITERKRAE
                     EALRESEERFAKTFAASPVGINIFGLQDGRSRDVNPSFLKIVGYTREEVVGRTAAEMN
                     LFVDPQMRAGWIQDLQAGLPVVNQPARIRRKSGEIRHILASLEVIEVRGDRMGLVIAA
                     DITDREEAEQAHAGLQAQLLQAQKMESVGRLAGGVAHDFNNILTVQRGYCEILKKGLK
                     EDDPISKGLAQIDRCAERAASLTRQLLAFSRRQTLQPKVIEMNVLVRDLDRMLRRLIG
                     EDVELSTVLSRTPTMV"
     gene            complement(2610..2978)
                     /locus_tag="VFH83_02780"
     CDS             complement(2610..2978)
                     /locus_tag="VFH83_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485315.1"
                     /translation="MAESAFSLGPLWTAGSTAQGKTAVLAWVRDVFLRQTNDISGFLF
                     EWLARPERKVTDSSGHLVEVRFLLLRVRLGHKDYVLRFDERDVEVWQDQPELKSKYHS
                     AVVRVIEQAPGSGAGGGPSR"
     gene            complement(3167..4165)
                     /locus_tag="VFH83_02785"
     CDS             complement(3167..4165)
                     /locus_tag="VFH83_02785"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET6485316.1"
                     /translation="MNAMDLHRDALVWDAHRDVAYEAPLSERFLQRWMIGVDLHLPRL
                     ARGGINVQTFALCIGPEIGRDYVAQALRELDQVFTILDAHRDDVVLVTRTADALQAKR
                     QGKIAVFLSFEGAEPILTELGLLRMFHRMGLRAMGLTWNYRNETADGGYEGREGYGLS
                     QFGAAVVKEMNRLGMVIDIAHATPQTMRDVLRASEQPVIHSHGGVRAVNPEHPRTLDD
                     AVLEQIARNGGVFCATTVPQAISTPAEEATLERYLDVIDHAVKVMGADHVGLGADFDV
                     YQSHLPYAIGHWLPGLEEADQWPEVTAGLLRRGHSEVTVRKILGENLQRVYQQVVG"
     gene            complement(4271..5038)
                     /locus_tag="VFH83_02790"
     CDS             complement(4271..5038)
                     /locus_tag="VFH83_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET6485317.1"
                     /translation="MGAIEERLPWRAILDYLAEVETAQTLDRFLVEASCGLEKLIPSD
                     VEVGFFHVNGPCLCQTGAGESVRRAYAEYYQYHLPWFEQGHKGLPSYLVGVQVVDLKR
                     IWGDTEFGTDFLPMTGSWKMMSSTFLSHSYVVNLTRSRLCPDFSEVEQSILSVVTPHI
                     NNLYSIFNKLGSATDQMPPPEMVHDALPVLSGREAEVVALLSRGLTAAEIATRLFISK
                     RTVDAHVAHVYDKLDIKSRSALRHKLSVALRPVGHPL"
     gene            5347..>5539
                     /locus_tag="VFH83_02795"
     CDS             5347..>5539
                     /locus_tag="VFH83_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485318.1"
                     /translation="MRWTLLAVLFAAVAATVSGQGLKMSVGLDAGFIPGVQERQTLAD
                     PSGIYLPAGTQFSEDYSYGA"
BASE COUNT         1000 a         1840 c         1715 g          984 t
ORIGIN      
        1 tcaccatcgt cggggtccgg gagaggaccg tggacagctc cacgtcctct ccgatgagcc
       61 gcctcagcat gcggtccagg tcccgtacca ggacgttcat ctcgatgacc ttgggctgca
      121 gggtctgcct tcggctgaac gcgagaagct ggcgggtcag ggaggcggcc ctctccgcgc
      181 agcggtcgat ttgcgccagt cccttggaga tcggatcgtc ctccttcagg cccttcttca
      241 gaatctcgca gtagccgcgc tgcaccgtaa ggatgttgtt gaagtcatgc gccacaccgc
      301 cggcaagcct gcccacggac tccatcttct gggcctgcag gagctgcgcc tggaggcccg
      361 catgggcttg ctcggcctct tcgcggtccg tgatgtcggc cgcgatgacc agacccatgc
      421 ggtccccgcg gacctcgatc acttccaggg aggccaggat gtggcggatt tcgccggatt
      481 tccggcgtat ccgggcgggc tggttcacca cggggagacc cgcctgaagg tcctggatcc
      541 agccggcccg catctgcgga tcgacgaaga ggttcatttc ggcagccgta cggccgacaa
      601 cctcctctcg ggtgtatccg acgatcttga ggaagctggg gttcacgtcc cgcgaccggc
      661 cgtcctggag accgaagatg ttgatcccta ccgggctcgc ggcgaacgtc ttggcgaagc
      721 gctcttccga ctcccgcaga gcctcctcgg cgcgcttgcg ctcggtgatg tcccggccca
      781 cgaccacgca gccgacgatg gctccggacc gatcgcggat cggggcgaag ctgtagctcc
      841 cgatccaggt ctctccggta tccttgcgcc gcaggcggta ctccgcgttg gcgacggtct
      901 ctccacggag cgcccggggg accgcccact gatccagggg cgcgggctcg ccgttcggca
      961 ggaagacgtc caggaacccg gggtactcgg ccagcgtcct ggcgcactcc tgcttgccgc
     1021 ggaaccggtg aaaggttgcg aaggcctcgt tgaagtcggt gaaggtccct tgtggatccg
     1081 ctaaggacac cgcgtcggtc atgctggaca gcgcggcttt gagacgtcct tcactcttgg
     1141 acagtgcctc cacggcacgc aggcgctccc gctcgctctc aatgctctgc agggcgaagc
     1201 cgatgtcctc cgtgatctct cgaaacaggg actgctcttc ctcgtcacgc gccaacgcca
     1261 gcggcagggc cgcaaccagg actccgtagt cacggcccga tgcccgcaga gccgcgacaa
     1321 gcgccgccat cccctggtac cccttggcca ggatgcaacc ggcgcagtcg gtggacagat
     1381 cgtggaggac aatcccatcc ggccgcgccg ccgcctggct gcagcacggc ggcaccatgc
     1441 cccgatccat gccggcttgc atgagacgga acgcctccgt gtcggatagc cgggcctcgg
     1501 ctaccgcatg gagacacccc ccggagtccc gggtcgcgac ccatgcggat tcgtagcccc
     1561 gggtcgcggt gagcaggtcg caggcctctt ggaggagggc cttcgggtcc ttctcgtgaa
     1621 cgatgagctg gttgacgctg cggatcgccc gcaggacttc tgtaagatgt ttctgacgaa
     1681 gctccgcctc tcttcggtcg gtgatgtcgc gcgccatgcc cagaaagaac cgttgcccgc
     1741 cggaatcgaa ccgcccgaat cgcacctcga ccgggaaggt cgtcccgtcc ttgcgtctct
     1801 ggtgtccata gagcgtgaac ggcctctcca gctcgatctt ggcccactcc ctctgggccg
     1861 cggggaggtc gaagtcctgc tcgacgtcca tgaccgtcat cgagagaagc tcgtctcggg
     1921 aatacccgag gctccggcac gcttgcgggt tgacctcgac aaaccgaccg ctgaagtcgt
     1981 gaacgaacat cgcctctccc gcctgctcca cgagggatcg gaagcgcgct tcattcgccc
     2041 tgagggattc ctcgcccacc ttgcgttcgg tgatgtcaca gaggaggaga aggacgccct
     2101 cagggaccgg gctgcagctc agatcgaacc actggggagg ccccgagcgc agggggagct
     2161 ccgcttccac gcgcgcggga gcggcctcct ccatgcagcg ccgcaggagc tggatcactt
     2221 tggtcgtctc tatccgtggg aaggcttcca tgatcgacag ccccaccagg ctgtcgcggg
     2281 ggcccccgat caagcgggcg gcggtttcgt tcgcatagaa gcacttccag tcacgatcga
     2341 agattgcgca gccctctaga aggctgtcca ggatggactg caagcgcctg gcgtcgggga
     2401 tcggcgagct cacgggtgca tcgaagccct gctcaactga cacaaacgcc tcctttgcga
     2461 actacttcgt aggccggccg cgttcgtcaa cgagcgatct tgtgccacag agattgtatc
     2521 acataaaggt attatgtcaa ggaaagcagt atctaaagcc acctcggatt gggtttctgg
     2581 tgtgagagag tctgcggaga gagctcacct catcgcgatg ggccgccacc tgctcccgag
     2641 cccggcgcct gctcaatgac cctgaccacc gcggaatggt acttcgactt caactcaggc
     2701 tggtcctgcc atacttcgac gtcgcgctcg tcgaagcgaa ggacgtagtc cttgtgcccc
     2761 aagcggacac gaaggagaag gaatcgcacc tccacgaggt gtcccgaaga gtctgttacc
     2821 ttgcgctccg ggcgcgccag ccactcgaag aggaacccgg agatatcatt cgtctggcgc
     2881 aggaatacgt ctcttaccca agccagaacc gcggtcttgc cctgagccgt cgagccggcg
     2941 gtccatagcg gacccaacga gaaagcgctt tcggccacag attccccttg aagagcgggt
     3001 ccccttcacc agaatccgcg gcttcctccc tccgtgccag tccgcaccga ccaggcaagt
     3061 agtatatact tcaatctggc ccgagggttc aacgggggca tctcccaccg gagctgacct
     3121 cgtccgcccg ctcagacgtc ccgtgcccgg gcaggcaacc cccgcctcag ccgaccacct
     3181 gctggtacac gcgctggaga ttctcaccga ggatcttccg gacggtgacc tctgagtgcc
     3241 ctctgcgaag gagcccggcc gtgacctcgg gccactggtc cgcctcctcg agacccggca
     3301 gccagtgccc gatggcatag ggaaggtggc tctggtacac gtcgaagtcc gcccccagac
     3361 ccacgtgatc cgcacccatc accttcacgg catggtcgat gacgtcgaga tatcgctcga
     3421 gggtggcctc ctcggccggt gtcgaaatgg cttgcggcac ggtcgtcgcg cagaagacgc
     3481 cgccgtttcg ggcgatctgc tccagcaccg catcgtccag ggtacgggga tgctcagggt
     3541 tcaccgcgcg gacacctccg tgggaatgga tcaccggctg ctcgctcgcg cgaagcacgt
     3601 cccgcatggt ctgtggcgtg gcatgagcga tgtcgatcac catcccgagc cggttcatct
     3661 ccttcaccac cgccgcaccg aactgggaca ggccgtagcc ctcgcggccc tcgtagcccc
     3721 cgtccgcggt ctcgttgcgg tagttccacg tcagccccat ggcgcgcagt cccatgcggt
     3781 ggaacatgcg caacaggccc aactcggtca ggatcggctc ggcaccctcg aagctgagga
     3841 ataccgcgat cttcccctgg cgcttcgcct gcagcgcatc cgccgtccgg gtcaccagca
     3901 cgacgtcgtc ccggtgcgca tcgaggatgg tgaagacctg atccagctcc cgcagggcct
     3961 gggcgacgta gtccctaccg atctcgggac cgatgcacag cgcaaacgtc tggacgttga
     4021 tccccccgcg cgccagtcgg ggaaggtgca gatccacccc gatcatccag cgctggagga
     4081 agcgctcgga gagaggcgcc tcataggcaa cgtcgcggtg agcgtcccag acgagcgcat
     4141 cgcggtgcag gtccatcgcg ttcatgctct tggagtcctc cccggcctca ctatagccgt
     4201 gagggcggat cgcgcaaggg cagccatgtc gaatgtcctt ccggtcggga tacgggaccg
     4261 gtcgcgggga ctacagcgga tgtccgaccg ggcgaagcgc cacagagagc ttgtgtctca
     4321 gggcgctcct cgacttgatg tccagcttgt catagacgtg ggctacatgg gcgtcgacgg
     4381 tacgcttgct gatgaaaagg cgagtcgcga tctcggccgc cgtcaggccg cgagacaaca
     4441 gggccacgac ttccgcctca cgtcccgaga ggacgggcag cgcgtcgtga accatctccg
     4501 gtggtggcat ctggtccgtc gcggagccca gtttgttgaa gatgctgtac aggttgttga
     4561 tgtggggcgt cacgacagac aggatggact gctctacttc actgaagtcc ggacagagcc
     4621 gcgagcgggt cagattgacg acgtacgaat ggctcagaaa cgtcgacgac atcatcttcc
     4681 aactaccggt catcggcagg aagtctgtac caaactcggt atccccccag attcgcttca
     4741 ggtccaccac ctgtacgccc acgagatagg acgggagccc cttgtggcct tgttcgaacc
     4801 agggaagatg gtactggtag tactcggcgt acgctcgccg aaccgactcg cctgctcccg
     4861 tctggcacag acatgggccg ttgacatgaa agaagccaac ctcgacgtca gagggaatca
     4921 gcttctcgag gccgcaagag gcctcgacaa gaaaacggtc cagcgtctga gcggtttcta
     4981 cctcggcaag gtagtccagg atcgccctcc acgggagacg ttcctctatg gcacccatcc
     5041 taccctccgc gcgagcgaac cgtggccaga ctcgacccag accggcaggt acatgcacca
     5101 gggtaatcca cgcgtcggat atgtcaatat ctccatcacc acaataggta aaaaagaccg
     5161 atgcgaatgt catagcggca gatcagaatc gtccccgtga tccaaaacgt ccattcagaa
     5221 agtcgaaggt tgccggctcg ccacctggcc gcagtcggcg agccggcgct cctccaccgg
     5281 agccctgatt ggcggcgctc gcagggggtc catctccaca accctgcaag acgaggatgg
     5341 gccccggtga ggtggaccct gctcgcggtg ctgtttgccg cggtcgctgc cacggtctcg
     5401 gggcagggtc tcaagatgag cgtagggctg gacgccggat tcattccggg agtccaggaa
     5461 cgccagaccc tggcggatcc gtccgggatc taccttccgg cgggcactca gttcagcgag
     5521 gactatagct atggcgcgg
//
LOCUS       DASRZS010000117         2479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1988274, whole genome shotgun sequence.
ACCESSION   DASRZS010000117 DASRZS010000000
VERSION     DASRZS010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988274"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1566
                     /locus_tag="VFH83_02800"
     CDS             310..1566
                     /locus_tag="VFH83_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET6485319.1"
                     /translation="MANSKIKDPGLAAAGKLRIEWAESRMPVLMALREKHSKARSLKG
                     MKVAGCLHVTKETAVLVRTIIAAGADVSWSGCNPLSTQDDVAAALAADGVSIFAWHGQ
                     SREDFYWAIDQTLAFSPTLTLDDGADLIFRVHSARADLAKGILGGTEETTTGVHRLRA
                     MGDAGKLLYPVVAVNDAATKWDFDNVHGTGQSSIDGILRATSILLAGKTFVVAGYGHC
                     GRGVANRARGMGSLVVVTEVDPIQALRATMDGFEVMPMDEAASIGDVFITATGVKDII
                     VKRHFERMKDGAVVCNTGHYDCEVNIPDLVSLSRSVREIRANNEEHTLGDGRRIYLLA
                     KGRLVNLAAAEGHPSEVMDMSFANQLLSLIRLAKEGRGMEHRVYTIEPSQDREIATVK
                     LSTMGRRIDTLTAAQAAYLTDFASGT"
     gene            1563..1802
                     /locus_tag="VFH83_02805"
     CDS             1563..1802
                     /locus_tag="VFH83_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485320.1"
                     /translation="MTPYRACRTCGKYVPYPELLQRAYCSEDCMRTFASCPNCGRYFT
                     RGKGFDGERCSKDCAVHYQILRRYGPQPVTVVTEV"
     gene            1807..2454
                     /locus_tag="VFH83_02810"
     CDS             1807..2454
                     /locus_tag="VFH83_02810"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET6485321.1"
                     /translation="MNLIFLGPPGAGKGTLAGHVSAEYKIPQISTGDIFRDAIKRETE
                     LGKKVKEIVGRGDLVPDELTVSLVRERLSQADARSGYILDGFPRTIPQAEALQGFQTL
                     DAVINFTIGDELVVKRLSGREICKSCGAIYHVTNMPSKVKGVCDKCGGPLYTRPDDSL
                     ESITNRLEVYRKQTEPLIQYYGKKGLRKDIDSSSTPEKTMVQIQAVLDKIKKPGT"
BASE COUNT          488 a          797 c          804 g          390 t
ORIGIN      
        1 cctcattctc cccttccctt gactgtcaaa gatccagtgc gctcggaccc gccgtttccc
       61 ggctgcccct cacgcacgtt gccccagaga cggtacttcc cccctcacat ggagtgcaga
      121 ggttcgtacc gcacgaaggc aaagaggggt atatacgcat tgcccggacc tgtcaagtac
      181 ctccgctcga aaaaaggaga aagaatttcg gggcacgccg cagcagccgc agggaccgcc
      241 caaaacgccc aaaaccactc gccttgacgc ggcagatgcc tcacactact atcgcgaaga
      301 ggaggtgtca tggcgaatag caagatcaag gatcctgggt tggccgccgc cggaaagctg
      361 cgcatcgagt gggccgagtc ccggatgccc gtccttatgg cgctgcggga aaagcactcc
      421 aaggcccgct ctctcaaggg catgaaggtg gcagggtgcc tgcacgtcac caaggaaacg
      481 gcggttctgg tgcggacgat catcgcggcc ggcgcggacg tctcctggtc cggatgcaac
      541 ccgctgtcca cgcaggacga cgttgcggcg gcgctggcgg cggatggggt ctcgatcttc
      601 gcgtggcacg gccagtccag ggaagacttc tactgggcca tcgatcagac cctggcgttt
      661 tcgccgaccc tcacgctgga cgacggcgcc gacctgatct tccgcgtgca cagcgcgcgg
      721 gcggacctgg ccaaggggat cctgggcggg acggaggaga ccaccaccgg cgtccaccga
      781 cttcgtgcca tgggcgacgc gggaaagctc ctgtaccccg tggtggccgt gaacgacgcg
      841 gccacgaagt gggacttcga caatgtccac ggcacgggcc aatcgtcgat cgacggcatc
      901 ctgcgcgcca cctcgatcct gctggccggc aagaccttcg tcgtggccgg gtacggccac
      961 tgcggacgcg gcgtggccaa ccgggcccgg gggatggggt ccctggtcgt ggtcacggaa
     1021 gtcgatccca tccaggcgct gcgcgccaca atggacggct tcgaggtgat gcccatggac
     1081 gaggccgcat cgatcggcga cgtcttcatc accgccaccg gcgtgaagga catcatcgta
     1141 aagcgccact tcgagcggat gaaggacggc gcggtggtgt gcaataccgg ccactatgat
     1201 tgcgaggtga acatcccgga cctcgtgtcc ctgtcgcgct ccgtgcggga gatccgcgcg
     1261 aacaacgagg agcacacgct gggcgatggg cgccggatct acctcctcgc caagggaagg
     1321 ctggtcaacc tggccgccgc ggaaggacac ccctccgaag tcatggacat gtcgttcgcc
     1381 aaccagctcc tctccctgat ccgcctggcg aaggaaggtc ggggcatgga gcaccgggtg
     1441 tacaccatcg agccgagcca ggaccgcgag atcgccaccg tgaagctctc caccatgggc
     1501 cggcggatcg atacgctcac tgcggcgcag gccgcgtacc tcacggactt tgcgagcgga
     1561 acgtgacccc ctaccgggcg tgccgaacct gtggaaagta cgttccctac cccgagctcc
     1621 tgcagcgcgc gtactgcagc gaagactgca tgcgcacgtt cgcgagctgc cccaactgcg
     1681 gtcgctactt cacgcgcggc aagggattcg acggggaacg ctgctcgaaa gactgcgcgg
     1741 tgcactacca gatccttcgc cggtacggtc cgcagcccgt gacggtcgtg acagaggtgt
     1801 gaagagatga acctgatctt cctgggtccc cccggagccg gcaagggtac cctcgccgga
     1861 cacgtgagcg ctgagtacaa gatcccccag atctcgacgg gagacatctt ccgggatgcc
     1921 atcaagcggg agacggagct gggcaagaag gtgaaagaga tcgtgggacg gggagacctc
     1981 gtgccggacg agctcacggt gagcctggtg cgggagcgcc tctcccaggc ggacgcccgg
     2041 tccggttaca tcctggacgg cttccctcga accatccccc aagcggaagc ccttcaggga
     2101 ttccagaccc tggacgccgt gatcaacttc accatcggcg atgagcttgt ggtcaaacgg
     2161 ctctccgggc gggagatctg caagtcctgc ggcgccatct accacgtgac caacatgccg
     2221 tccaaggtga agggcgtctg cgacaagtgc ggcggtccgt tgtacacgcg gccggacgac
     2281 tcgctggagt ccatcaccaa ccggctcgag gtgtatcgaa agcagacgga gccgttgatc
     2341 cagtactacg gcaagaaggg gctgaggaag gacatcgact cctccagcac gccggagaag
     2401 acgatggtcc agatccaggc tgttctggac aagatcaaga agccgggcac gtagcgttca
     2461 tctcaggggc tggtcgggg
//
LOCUS       DASRZS010000118         4701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2370757, whole genome shotgun sequence.
ACCESSION   DASRZS010000118 DASRZS010000000
VERSION     DASRZS010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2370757"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFH83_02815"
     CDS             <1..283
                     /locus_tag="VFH83_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET6485322.1"
                     /translation="SYGELRCRVLHGVRTSAELYDRHVFDASAFQACVSREDTGDYRG
                     RVTDYLRAHPVDARSHCYLCGNCAMIHDAFDILRGQGVPAARLHTEVYF"
     gene            280..1707
                     /gene="miaB"
                     /locus_tag="VFH83_02820"
     CDS             280..1707
                     /gene="miaB"
                     /locus_tag="VFH83_02820"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET6485323.1"
                     /translation="MKYCLLHLGCQMNQSDAERVRTVIEAMGYQRTESEEEADLLGVV
                     SCSVRQRAIDRAYALVRKWNSWKDHRTLLTFATGCILPVDRERFLKQFDFLFPIDELP
                     QLPDMIRQYGVVTPASGAHVSTREAPPAPADGGGLLTLTDASRYYWQIKPRYSSTIEA
                     YVPIQNGCDKFCTFCAVPYTRGRELSRPSAEVLEEVSSLVERGYRSITLLGQNVNSYG
                     LDRKGAEISFADLLRRIGEMGNGSGRRFWVYFTSPHPRDMTEEVIRAVARYPCLAKQI
                     HLPLQSGDDKVLIRMNRNYRVDEYRAVVQSIRALLPEATLFTDIIVGFTGETEEQFEN
                     TRKAMREFQYSMAFVAQYSPRPGAASYRWPDDVPHEEKKRRLRELSEELRASALAWNR
                     RMVGRSVAVLVDGRDRKPGWLSGRTEGRIPVRFPSADESLIGAIVDVTITETAPLSME
                     GELAIPGAPSAAPRYPALPSSSRHP"
     gene            complement(1640..2551)
                     /locus_tag="VFH83_02825"
     CDS             complement(1640..2551)
                     /locus_tag="VFH83_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6485324.1"
                     /translation="MATITPFLWFDGQAEEAAVFYTSIFEGSKVGQKSYYGPSGPLPR
                     GTVMVVEFQLEGQSFTALNGGPDFHFTPAISFYVRCGTVDTIDALWRKLSDGGTVLME
                     LQRYPFSERFGWVGDRYGVTWQLNLEPMTPGPQPRRAALPQRIVPFLLFVGKQQGRAE
                     EAMNRYTSIFPGSKVELIQRHPAGGAEPEGSVQHARFLLDGQEFMAMDSALQHEFTFT
                     PALSLYVRCQTQAEIDAFWSKLCDGGAEVQCGWLTDRFGVSWQIVPSILAELVGDNTT
                     ARSHRVMDAVMKMVKLDIEELRKAHRG"
     gene            complement(2610..3473)
                     /locus_tag="VFH83_02830"
     CDS             complement(2610..3473)
                     /locus_tag="VFH83_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6485325.1"
                     /translation="MDVHSVVVTGVSTGIGRGISQVLVRHGVHVFGSVRSRKDADSFA
                     RDLGSSVTPLVFDVTDGAAVEAAALTVRQTLAGRTLSGLVNNAGVAVFGPLMHVPIAE
                     VRRQMEVNLLGPLLVSQRFLPLLGADRSLTGPRGRIINIGSIGGKIAFPFLGPYAASK
                     FALEGLSESLRRELMLYGIDVIVVGPGAVATPIWGKGEQADTTGYAATEYGETLRRFG
                     DYMIQNGKTGLPPERIGAVVWSALSSRRPRVRYAVVPRAFSSWVVPRLLPKRMVDRTI
                     ARSLGWRQRAS"
     gene            complement(3555..>4701)
                     /locus_tag="VFH83_02835"
     CDS             complement(3555..>4701)
                     /locus_tag="VFH83_02835"
                     /inference="COORDINATES: protein motif:HMM:NF019368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HET6485326.1"
                     /translation="GEARIGATPSSDLSWTRLPEPKVGGYDIEPDGREDVKVENRWFS
                     LVIDGHHGDIRKLVDRRTGVDVFSGHAAAPIVVDDPTDTWSHGVFSFHEERGRFGAAR
                     VRVVEEGPVRIVVRARSSFGSSTLLQDFILYREIPRITVRAVVDWHEKLSLLKISFPV
                     GVQKPAATYEIPYGHIQRPCNGEEEPGQNWVDVSGEASVGGRPTSYGLSVLNDCTYSF
                     DVRGGDLRLTILRSPVYAHHTPTVLEENGDYVYMDQGRHAFSYELVPHTGSWQDGETV
                     RSAQVLNAPLVALVEHAHAGDLPAAASHIQVDAPNVIATVLKQREDSRDLVLRCYESV
                     GRATEATIDLPLLKRKIPVSLKPCEIKTFVIPREPSKPVAPTDFLED"
BASE COUNT          799 a         1598 c         1537 g          767 t
ORIGIN      
        1 cagctacgga gagctccgat gccgggtgct gcacggcgta cggacctccg ccgagctcta
       61 cgacagacac gtgttcgacg cctccgcctt ccaggcgtgc gtgagtcggg aggacacggg
      121 ggactaccgc gggcgggtca ccgactacct gcgggcgcac ccggtggatg cccgcagtca
      181 ctgctacctc tgcggcaact gcgcgatgat ccacgacgcc ttcgacatcc tgcggggcca
      241 gggcgttccc gccgcccgcc tgcacaccga ggtctacttt tgaagtactg tctcctccac
      301 ctgggctgcc agatgaacca gtccgacgcg gagcgcgtcc gcaccgtgat cgaggccatg
      361 ggctaccaac ggaccgaatc ggaggaagag gcggacctgc tgggcgtcgt gtcctgctcc
      421 gtccgccagc gcgccatcga tcgcgcctac gccctggtcc gcaagtggaa ctcctggaag
      481 gaccatcgga cccttctcac cttcgccacg ggctgcatcc tgcccgtgga ccgcgagcgg
      541 ttcctgaagc agttcgattt cctcttcccg atcgacgagc ttccccagct tccggacatg
      601 atccgccagt acggcgtggt gacgcccgcc agcggggcgc acgtttcgac gcgcgaagcg
      661 ccacccgcgc cggccgatgg aggtgggctc ctcaccctca ccgacgccag ccggtactac
      721 tggcagatca agccccggta cagctccacc atcgaagcct acgtaccgat ccagaacggc
      781 tgcgacaagt tctgcacctt ctgcgcggtc ccctataccc gtgggcgcga gctctcgcgg
      841 ccctccgcgg aggtgctgga ggaggtctcc tccctggtcg agcgcggata ccggtccatc
      901 acgctcctgg ggcagaacgt gaactcctat gggctggatc ggaaaggggc cgagatctcc
      961 ttcgccgatc tgcttcgcag gatcggggag atggggaacg gctcgggccg ccggttctgg
     1021 gtgtacttca cctcgcctca tcctcgggac atgaccgaag aggtgatccg cgcggtggcg
     1081 cggtatccgt gtctggccaa gcagatccac ctgcccctgc aatcgggaga cgacaaggtc
     1141 ctgatccgca tgaaccggaa ctaccgtgtg gacgagtacc gcgcggtggt ccagagcatc
     1201 cgggccctgc tcccggaggc cacgctgttc accgacatca tcgtcggctt caccggggag
     1261 accgaggagc agttcgagaa cacacgcaag gccatgcggg agttccagta ctccatggcg
     1321 ttcgtggccc agtactcgcc gcgaccggga gccgccagct accggtggcc cgacgacgtg
     1381 ccccatgagg agaagaagcg acgactgcgc gagctctcgg aggagctgcg cgcaagcgcg
     1441 ctggcatgga accgacgcat ggtgggccgc tccgtagccg tcctggtgga cggccgcgac
     1501 cgaaagcccg ggtggctctc cggcaggacg gagggacgga tccccgtccg attccccagc
     1561 gccgacgagt cgctgatcgg ggcgatcgtg gacgtgacga tcaccgagac ggcgcccctg
     1621 tccatggagg gggagttggc tatccccggt gcgccttccg cagctcctcg atatccagct
     1681 ttaccatctt catcacggca tccatgaccc gatgggatcg agcggtggtg ttgtccccaa
     1741 ccagctccgc gaggatcgag ggaacgatct gccacgagac cccgaaccgg tcggtgagcc
     1801 agccgcactg gacctcggct cctccgtcgc acagcttcga ccagaacgcg tcgatctctg
     1861 cctgcgtctg acaccgcacg tagagggaga gcgcgggcgt aaaggtgaac tcgtgctgga
     1921 gggcgctgtc catggccatg aactcctggc cgtccagcag gaagcgcgcg tgctgcacgg
     1981 agccctccgg ctcggctcct cccgccgggt gacgctggat cagctccacc ttggagccgg
     2041 ggaagatgga cgtgtagcgg ttcatcgctt cctcggcccg gccttgctgt ttgccgacga
     2101 agaggagaaa cgggacgatc cgctggggga gcgccgcccg ccgcggctgc ggcccgggag
     2161 tcatgggctc caggttgagc tgccaggtga cgccgtagcg gtctcccacc cacccgaacc
     2221 tctcgctgaa gggatacctc tgcagctcca tgagcacggt gccgccgtcg ctgagctttc
     2281 gccacagcgc gtcgatcgtg tccaccgtcc cgcagcgaac gtagaaggag atggcaggag
     2341 tgaagtgaaa atcgggtccg ccgttcagcg cggtgaagct ctggccctcg agctggaact
     2401 cgacgaccat cacggtgcct cgcggaaggg ggccggacgg gccgtagtag gacttctgtc
     2461 ccaccttcga tccctcgaag atggacgtgt agaagacggc ggcttcctcc gcctgtccgt
     2521 cgaaccagag aaatggcgtg atcgtggcca tgaaggctcc tttcctcgac cggcgtggct
     2581 gcgacgggcc cggctgcgag gcgcgccggc tacgacgcgc gctggcgcca gcccaggctc
     2641 cgggcgatcg tgcggtccac catccgcttg ggaagcagcc gcgggacgac ccatgagctg
     2701 aaggctcttg ggactaccgc gtagcggacg cgaggacgtc gggaggaaag cgcggaccat
     2761 accaccgcgc caatgcgctc aggcggaagg ccggtcttcc cgttctggat catgtagtca
     2821 ccgaacctgc gcagggtttc cccgtactcg gtggcagcgt acccggtggt atccgcctgc
     2881 tcgcccttgc cccagatggg ggtggccacc gctccgggac cgaccacgat cacgtcgatg
     2941 ccatagagca tgagctctcg ccgcaggctc tcggagaggc cctccagggc gaacttggac
     3001 gcggcgtacg gtccaaggaa gggaaaggcg atcttgccgc cgatggaccc gatgttgatg
     3061 atccggcccc gcgggcccgt gagagaccga tccgctccca agagcggcag aaacctctgc
     3121 gacaccagga gcggtcccag gaggttcact tccatctgtc ggcggacttc ggcgatgggt
     3181 acatgcatga gaggcccaaa gaccgccacg cccgcgttgt tcacgaggcc gctcaaggtc
     3241 cggcccgcaa gggtttgacg cacggtcagg gcggcggcct ccacggcagc gccatcggtc
     3301 acgtcgaaca cgagcggggt gacggagctc cccagatccc tggcaaagga atccgcgtcc
     3361 ttccgcgagc ggacgctgcc gaagacgtgc accccgtgcc ggaccaggac ctgggagatg
     3421 cccctgccga tgcccgtgga gacgccggtt accaccaccg aatggacgtc catgatgaga
     3481 ggaaggtact gagaatgggg gccccggcga agaggtttcg cctggggacc ccgtggatgc
     3541 gccaggtagg gccctcagtc ctccaggaaa tccgtcggcg ccaccggctt ggagggctcc
     3601 cgcgggatca cgaacgtctt gatctcacac ggcttcaggg agaccgggat cttccgcttc
     3661 agcaagggca gatcgatcgt ggcctccgtg gcccgtccca cgctctcgta gcagcgaagc
     3721 accaggtccc gcgaatcctc cctctgcttc agcacggtcg cgatgacgtt cggtgcgtcc
     3781 acctggatgt ggctcgcggc cgcgggcaga tcacccgcgt gcgcgtgctc taccagggcg
     3841 acgagcggcg cgttcagcac ctgggcgctt ctcacggttt ccccgtcctg ccaggacccc
     3901 gtgtggggga ccagctcgta ggagaaggcg tgcctcccct gatccatgta cacgtagtcc
     3961 ccgttctcct ccagcacggt cggcgtgtgg tgagcgtaga ccggacttcg caggatggtc
     4021 agccgaagat cgccgccccg aacatcgaag ctgtaggtgc agtcgttgag aacgctcagg
     4081 ccataggagg tcggtcttcc accgacactc gcctctccgg atacgtctac ccagttctgg
     4141 cccggttcct cttccccgtt gcacggcctc tggatgtggc cgtaggggat ctcgtaggtc
     4201 gccgcgggct tctgcacgcc caccgggaag ctgatcttca agaggctgag cttctcgtgc
     4261 cagtccacta cggcgcgaac ggtgatccgc gggatctccc gatagaggat gaagtcctgc
     4321 agaagggtcg aggatccgaa gctgctgcgg gcgcggacca cgatccgcac gggcccctcc
     4381 tccaccacgc gcacgcgcgc cgcaccgaac ctgccgcgct cctcgtggaa ggagaacacc
     4441 ccatggctcc acgtgtcggt ggggtcatcc acgacgatcg gggccgcggc gtgtccgctg
     4501 aacacgtcca cgcccgtgcg ccggtcgacg agcttccgga tgtccccgtg gtgaccgtcg
     4561 atgaccaggg agaaccaccg gttctccacc ttcacgtcct cccgaccgtc gggctcgatg
     4621 tcgtagccgc ccaccttggg ctccggcagg cgggtccagg agagatccga tgacggcgtc
     4681 gcgccaatgc gggcctcccc c
//
LOCUS       DASRZS010000119         4549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_835086, whole genome shotgun sequence.
ACCESSION   DASRZS010000119 DASRZS010000000
VERSION     DASRZS010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835086"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VFH83_02840"
     CDS             complement(<1..414)
                     /locus_tag="VFH83_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HET6485327.1"
                     /translation="MHRITIEADLGTQTISRHIYGHFAEHLGRCIYEGIWVGEDSPIP
                     HTGGIRNDVVEALSRLRVPNLRWPGGCFADTYHWRDGIGPRDQRPSMVNVHWGGVTEN
                     NHFGTHEFLDFCDRIGAEPYICGNVGSGSVREMSEW"
     gene            499..1521
                     /locus_tag="VFH83_02845"
     CDS             499..1521
                     /locus_tag="VFH83_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6485328.1"
                     /translation="MEHRLLGRSGLSVPVLALGTATFGGRGEFFKPFGTTGVAEARRL
                     VDICLEAGVTLFDSADGYSGGLAEEILGQALEGRRDRVLISTKATFRSGDDPNDVGSS
                     RFHLIQAVESSLRRLRTDHIDLFELHAFDAMTPVEETLRTLDDLVRAGKIRYIGCSNF
                     SGWHLMKSLAASDRLGLSRYVAHQAYYSLIGRDYEWELMPLALDQGVGTVVWSPLGWA
                     RLTGKYRRGAQRPAVSRINSPAAEGRSVPDEYLYRVVDAIDEVAKETGKTVPQIALNW
                     LLQRPSVSTLIVGARNEEQLRDNLGAVGWKLTPQQVARLDAASETPRPYPYWHQQGFE
                     RNPPPV"
     gene            complement(1559..2653)
                     /locus_tag="VFH83_02850"
     CDS             complement(1559..2653)
                     /locus_tag="VFH83_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6485329.1"
                     /translation="MGVTQKDIADALKISVVTVNRAMNGAGYVSRKLRERILNHAKAV
                     HYVPHKASQVLVRNKVRRIALFSSALPHYFWNDIRTGVSIAAEQIQGFHYEVAYHTIT
                     ERKSGLYVRRLQEEIEAGVQAVGVVNQWIFDMKAIFSTIERAGLPYITLNVDAPESKR
                     LCYIGPDYRAGGRLAAEYVGKALVFRKSPRVLVVTSQDKEPADASAPDINRLRYEGFR
                     EVMRDRFPSVRLDTGTLTMDIRSRDAPEQIQEVLRSHQRKADAVYFIPAFNAQFIDAF
                     QRVGPRSALVVLNDLDASAGQFLSGNALAAVIYQNPVLQGYYAVKVLENLLESGHPPE
                     IRQIHLTHSVLLNENKDLNRNHYLFTGMLE"
     gene            complement(2644..>4549)
                     /gene="yicI"
                     /locus_tag="VFH83_02855"
     CDS             complement(2644..>4549)
                     /gene="yicI"
                     /locus_tag="VFH83_02855"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HET6485330.1"
                     /translation="DADGRHFLRERLSLAVGECVYGLGERFGPLVRNGQSVDIWNEDG
                     GTSTDIAYKNIPFYLTSRGYGVLVNHPEAVSFEVGSEAVSKVQFSVAGERLEYLVVGG
                     GSPKEVLRRYTRLTGRPALPPAWSFGLWLTTSFTTDYNESTVSSFVQGMRDRGIPLHV
                     FHFDCFWMKEFQWCDLEWDRDQFPDPAGFIRRLKDTGLHICLWINPYIAQKSRLFDEG
                     SAAGLLLKRPDGSIFQWDRWQAGMGLVDFTNPKAVAWYREKLRALLRMGVDSFKTDFG
                     ERIPVDVVYADGSDPARMHNFYSYLYNKTVFDLLVEEKGPGKAAVFARSATVGGQSFP
                     VHWGGDSSATFESMAESLRGGISLGLSGFGFSSHDIGGFESTATPDLYKRWSAFGLLS
                     SHSRLHGNESYRVPWLFDEEAVDVLRAFARLKCRLMPYLYAAACQASRDGIPVLRAMV
                     IEFADDPACAYLDRQYMLGDSLLVAPVMDPGGSVGYYLPAGRWTDFLTGEVREGGSWR
                     AEKHGYQSLPLLARPGTLIAVGIDENRVEYDYANQVTFHAFEIPDGCRATATVYADGG
                     AERCSVTVARSGGVLAARAQGAVGPWSLLLRGIRSVRGVSGGQAQEHALGTRIAVAAG
                     GAAVDVRAWE"
BASE COUNT          766 a         1536 c         1485 g          762 t
ORIGIN      
        1 ccactccgac atttcccgga cactgccgga cccgacgttc ccgcagatgt acggctcggc
       61 tccgatcctg tcgcagaagt cgaggaactc gtgggtgccg aagtggttgt tctccgtgac
      121 gccaccccag tgcacgttga ccatgctcgg tctctggtcc cggggcccga tcccgtcccg
      181 ccagtggtag gtgtccgcga aacagcctcc gggccagcgg aggttcggga cccgcagacg
      241 ggacagcgcc tccacgacgt cgtttcggat gccccccgta tgcgggatcg gggagtcttc
      301 accgacccaa atcccctcgt agatgcatct gcccaggtgc tccgcgaagt ggccgtagat
      361 gtgccgtgag atggtctggg tgcccaggtc cgcctcgatc gtgatccggt gcatggaatg
      421 acgctccttc ccgcgcccgc cgcggtcccg gttgcccacg aatggacgca gtagtacctt
      481 gcacgaaaag gagctgccat ggaacaccgt ctgctgggtc gctcgggtct ctccgtgccg
      541 gtcctcgccc tggggaccgc gacgttcggc ggccgcggag agttcttcaa accgttcggc
      601 accacgggag tggcggaggc caggcgcctg gtggacatct gcctggaggc gggcgtcacg
      661 ctgttcgact ccgcggacgg ttactcgggc gggctggctg aggagatcct ggggcaagcc
      721 ttggagggcc gacgggaccg ggtcctcatc tccaccaagg ccacgttccg aagcggcgac
      781 gacccgaacg acgtggggtc ctcccgcttc catctcatcc aggccgtgga aagcagcctg
      841 cggcggctgc ggaccgacca catcgacctg ttcgagctcc acgcgttcga cgccatgacc
      901 cccgtcgaag agacgttgag gacgctggat gaccttgtgc gcgccggcaa gatccgctac
      961 atcggctgct ccaacttctc cggctggcac ctgatgaagt cccttgccgc ttcggaccgc
     1021 ctcgggctct cccgctacgt cgcgcaccag gcctactact ccctgatcgg gcgggactac
     1081 gaatgggagc tcatgccgct ggcgctcgac cagggcgttg ggaccgtggt ctggagcccc
     1141 ttggggtggg cgcgcctgac cggcaagtac cgccgcggcg ctcagcggcc cgccgtgagc
     1201 cgcatcaact ccccggccgc ggaaggacgc tcggtccccg acgagtatct ctaccgtgtg
     1261 gtggatgcca tcgacgaggt ggcaaaggag accggcaaga cggttccgca gatcgcgctc
     1321 aactggctgc tgcagcgacc gagcgtgagc acgctcatcg tcggggcgcg gaacgaggag
     1381 cagctccgcg acaacctcgg cgccgtcggc tggaagctga ccccccagca ggtggcccgc
     1441 ctggacgcgg ccagcgagac accccgcccc tacccctact ggcaccagca gggcttcgag
     1501 cgcaacccgc cgccggtgtg aggggagagc ggatctcctt cgacgggatg gccttcggct
     1561 actccagcat ccccgtgaaa aggtagtggt ttcggttcag gtccttgttc tcgttcagca
     1621 gcacgctgtg cgtcaggtgg atctgtcgga tttcgggcgg atgccccgat tccagcaggt
     1681 tctccaggac cttcaccgcg tagtatccct ggagcacggg attctgatag atgacggcgg
     1741 cgagcgcgtt ccccgaaaga aactggcccg ctgaagcgtc cagatcgttc agcacaacca
     1801 acgcgctcct gggccccacg cgctggaaag cgtcgatgaa ctgggcgttg aacgcgggga
     1861 tgaagtacac ggcatccgcc ttcctctggt gggaacggag cacctcctgg atctgctccg
     1921 gcgcgtcccg ggagcggatg tccatggtca gcgtgccggt atccagtcgg accgatggga
     1981 accggtcccg catcacttca cggaagccct cgtaccgcag ccggttgatg tccggggcgc
     2041 tcgcgtccgc gggctccttg tcctggctgg tcacaacgag cacgcgcggg ctcttgcgga
     2101 acacgagcgc cttgccgaca tactccgccg ccagtctccc acccgcccga tagtccggcc
     2161 cgatgtagca cagccgcttg ctctcgggcg cgtcgacgtt cagggtgatg tacgggaggc
     2221 cggcccgctc gatcgtcgag aagatggcct tcatgtcgaa gatccattgg ttcacaacgc
     2281 ccaccgcctg gacgccggct tcgatctctt cttgaagcct cctcacgtac aggccggatt
     2341 tgcgctcggt gatggtgtgg taggccacct cgtagtggaa gccctggatc tgctcggcgg
     2401 ctatggagac tccggtgcgt atgtcgttcc agaagtagtg cggaagcgcc gaagagaaca
     2461 gggcgatccg gcgcaccttg ttgcggacga gcacctggga ggccttgtgc gggacgtagt
     2521 ggaccgcctt ggcgtggttg aggatccgct cccgaagctt gcgggagacg tagccggccc
     2581 cgttcatggc ccggttcacc gtcaccacgg agatcttcag ggcgtcggcg atgtcctttt
     2641 gggtcactcc catgcgcgaa cgtccacggc ggcgccaccg gcggcgacgg cgatccgcgt
     2701 tcccagcgcg tgctcctggg cctgaccgcc cgagacgccg cgtacggatc ggatccctcg
     2761 caaaaggagc gaccacggcc cgaccgctcc ctgcgcccgg gcggcaagca ccccgccgga
     2821 ccgagccacc gtcacggagc aacgttcggc gccgccgtcg gcgtacaccg tggcggtcgc
     2881 tcggcagccg tccgggatct cgaacgcgtg gaacgtcacc tgattcgcat aatcgtactc
     2941 cacccggttt tcatcgatgc cgaccgcgat gagcgttccg ggtctcgcca ggagcggcag
     3001 gctctggtag ccgtgcttct ccgcccgcca gcttccacct tcccggacct cgccggtgag
     3061 aaagtccgtc cagcgcccgg ccggcaggta gtagccgacg gacccgcccg gatccatgac
     3121 gggcgcgacc aggagcgaat cgcccagcat gtactggcga tcgagatacg cgcaggccgg
     3181 gtcgtcggca aactcaatga ccatggcgcg caggacggga atgccatcac gggacgcctg
     3241 acaggcggcg gcgtacaggt agggcatgag acggcacttc aggcgggcga acgccctcag
     3301 aacgtccacc gcctcctcgt cgaacagcca ggggacgcga taggattcgt tgccgtgaag
     3361 gcggctgtgg gagctcagga gcccgaaggc ggaccatcgc ttgtagaggt ccggcgttgc
     3421 cgtgctctcg aagccgccga tgtcatggct cgagaagccg aagccggaga gccccagcga
     3481 gatcccgccg cgcaggctct ccgccatgga ctcgaaggtg gccgaggagt ctccgcccca
     3541 gtgaacgggg aagctctggc cgcccacggt ggcggaacgc gcaaagacgg ccgccttgcc
     3601 cgggcccttc tcctccacca ggagatcgaa gaccgtcttg ttgtagaggt agctgtagaa
     3661 gttgtgcatc cgcgccgggt cggacccgtc cgcgtacacc acgtccaccg ggatgcgttc
     3721 cccgaagtcg gtcttgaagc tgtccacgcc catcctcagg agcgcccgaa gcttctcccg
     3781 gtaccaggcc acggccttgg ggttggtgaa gtccaccaga cccatgccgg cctgccaccg
     3841 gtcccactgg aagatgctgc cgtccggccg cttcagcaat agcccggcgg cggatccttc
     3901 gtcgaagagc ctcgacttct gggcgatgta cgggttgatc cacagacaga tgtgaagacc
     3961 ggtgtccttg agcctgcgga tgaatccggc gggatccggg aactggtcgc gatcccactc
     4021 caggtcacac cactggaact ccttcatcca gaagcagtcg aagtggaaca cgtggagggg
     4081 gatgccgcgg tcccgcatgc cctggacgaa ggagcttacg gtgctttcgt tgtagtccgt
     4141 cgtgaacgag gtggtcaacc agaggccgaa ggaccacgcg ggaggaagcg cgggccggcc
     4201 cgtcagccgc gtgtagcgac ggagcacctc cttcgggctg ccgccgccca ccacgaggta
     4261 ctccagccgc tctcccgcaa cggagaattg gaccttggaa acggcctcgg accctacctc
     4321 gaaggagacc gcctcggggt ggttcaccag cacgccgtag ccgcggctcg tgagatagaa
     4381 cgggatgttc ttgtaggcga tgtccgtgga agtgccgccg tcctcgttcc agatgtcgac
     4441 ggactgtccg ttcctgacca gcggcccgaa gcgctccccc aacccgtaca cgcactctcc
     4501 caccgccagg gaaagccgct cccgcaggaa gtgccggccg tccgcgtcc
//
LOCUS       DASRZS010000120        11575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3144511, whole genome shotgun sequence.
ACCESSION   DASRZS010000120 DASRZS010000000
VERSION     DASRZS010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11575
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3144511"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..201
                     /locus_tag="VFH83_02860"
     CDS             43..201
                     /locus_tag="VFH83_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485331.1"
                     /translation="MKVYFYDAGGKGVTDISALDLSSENAAEAGWGGLTRFSGNIADV
                     VSELSSEG"
     gene            726..1736
                     /locus_tag="VFH83_02865"
     CDS             726..1736
                     /locus_tag="VFH83_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6485332.1"
                     /translation="MSDAKPRLLIDPSPRTMREIFTDQDLARVRGLYDVVWGRDDPIS
                     QEAFDREKRDLFAVACCGWRYGSIQEMARLRVLLDVCGGPPRPDQLDYASCFERRIAI
                     LNCSPAFGPVVAEMALGMALDAAREISFGDREFRAASEHYLHSGNVHTLSLFGSAVGL
                     IGYGSLARNLQPLLAPFRCRISVYDPWKTEAQLRGAGVTPATLDEVLRGSDVIFVLAQ
                     PTTGNKAMITRRHLELIQPHKVFVLISRSHVVDFDALTDLALQDRFKAAIDVFPEEPL
                     AKDHPIRKAPGAVLSAHRAGALPVALLDIGRMVADDLEAIIRGLPPWRMQRTEPELAQ
                     KL"
     gene            1751..2353
                     /locus_tag="VFH83_02870"
     CDS             1751..2353
                     /locus_tag="VFH83_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006962303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HET6485333.1"
                     /translation="MKVLVIGAKGTIGKAVVDALERRGHEVVRASRTSDIRVDLEDPK
                     SIQALFSSVGKVDAVVGCAGSGGWGPLATLSDQDFAFSLRSKLMGQVNLIRIAQSHVR
                     DGGSITVTSGTLATHPQPGGAALSLVNAGLDGFVRAAALELPRGIRVNVVSPPWVKET
                     LRAMKADESRGLAAADVAKAYLEAVEGRGNGEVLEPTRFL"
     gene            complement(2398..3339)
                     /locus_tag="VFH83_02875"
     CDS             complement(2398..3339)
                     /locus_tag="VFH83_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6485334.1"
                     /translation="MSANAEDERLYRIGIFARKARVSVRTVRFYDLEGLLVSRRRSLA
                     GFRLYDWRDFERLRRILALKALGLPLAEIRRILDTETADLSGILDRQSQELGGRIQVL
                     QGVVDAIRQVQIAATSHAVDWNLILRVAAAIGRESIPMNEFTLGEDRYREASLSVLYP
                     RGRKTVWARLAPALPVIVAVGLVAVQVALRQYVGAAVLAGGFLFLTIATLLVTRGRRG
                     TVQARATRLSTVPYRIRVLETGLELEYDKSSFHMPFENLHIGRRNPRFYHVTHELGLM
                     TYIPAASIGESEREILDRHVTTGPIDWKAKVASSGTS"
     gene            complement(3418..4440)
                     /locus_tag="VFH83_02880"
     CDS             complement(3418..4440)
                     /locus_tag="VFH83_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485335.1"
                     /translation="MQGKSLADLTHEAFDHGRRLVAPLAGFPGVEIVGSTIKLAQQNY
                     GEHYRAIRALVDRFQPDVAFPLMDLAVEANALGRYTVFPQEDSATVPKDRFSIDELER
                     LGRINISFDSRVLGYVEMMRMMSVTLPPAILRGGYAVGPFTLAGLMMGADEAAMATVT
                     EPDLLHRLCHFTTERIQEYVQMLISAGAQVICILEPSAVMLSPQQFSEFSAIYIRHIT
                     TSFKYSGINFVYHTCGNTTHLVEKMVRSGVNGLSLDSREAGVDLPTLSKQVPEDVVVI
                     GNISPTTTMRFGSPSDVQRDVEELLRQMDRFPNFVLSTGCDLPQETPEENLRVFMEAG
                     RAWHKR"
     gene            complement(4511..5182)
                     /locus_tag="VFH83_02885"
     CDS             complement(4511..5182)
                     /locus_tag="VFH83_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6485336.1"
                     /translation="MIDRGVALSAFPFLDSVPSASREQFLSQAIAKRLEPDQILVSGG
                     SECAYLPLVLEGTLRVYKVSSEGRELTLYRIERGESCVLTATCIMNHDGFPAIARAEG
                     PTLVALLPARLLSGLVEKHPAWRAFVFGLYARRLDSMLALVEEVAFRHVDQRIAGYLL
                     SAPSRVGAVRTTHARIALEIGTSREVVSRILKDFESRGILRVSRGAVLILLPEELARY
                     QSSTA"
     gene            5418..6647
                     /locus_tag="VFH83_02890"
     CDS             5418..6647
                     /locus_tag="VFH83_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET6485337.1"
                     /translation="MRYACAIDFGTTNSVVALAREGEPKPQVVASEPSCILITDDRST
                     QRLYAGNDAIAHYRGAADTTRFVKSMKSLLSDPGFQSTRVFGRTYTAAHLVRPVLSHL
                     KALAEGALGERIHRVILGRPVHFSAAWEHDDLAEGRLREAAVLAGFTEITFQKEPIAA
                     GWSFASTLQEERRVLVADLGGGTADFCLVVFRPDGRHQVLAVGGARIGGDDFDGRIMW
                     GRLISSFGYGSTYESWGKMLEVPIHIYRALCRWDRIPFLKESRTWRDLRYIHQGSTDK
                     PGIARLMALIRNDLGFPLYQRIAAAKHELSRAVVSTIRMDQDEVTLREELSRAQFESM
                     IAADVDQILGTASETLTTAGMKSTDVDICFLTGGCSLVPVVASRFARLFPGGRLSTEG
                     DAFTSVASGLAIYGLTA"
     gene            complement(6694..7653)
                     /locus_tag="VFH83_02895"
     CDS             complement(6694..7653)
                     /locus_tag="VFH83_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HET6485338.1"
                     /translation="MKAIVVREFGGPEVLKLEEAAEPLPGAGQVLIRVKAVGVNPTET
                     YQRSGMVRTAKPPYTPGSDAAGVVERVGSDVHGWRPGDRVYTAGTLSGSYAEMVLVRE
                     SQLHALPERVSFSQGAGINIPYATAFHALAHVAHARAGETVLIHGASGGVGIAACQLS
                     RNLALTAIGTAGTDAGRALILREGARHAVDHRAADQAEQIMKLAGGRGVDVILEMLAN
                     VNLGMDLKLLAPKGRVVIIGSRGDVTITPRDLMGRDASVHGMLLWNLSDADALSTYAA
                     LGEGLEQGTLQPVVGVELPLAEAAQAHRRILEPGAHGKIVLIP"
     gene            7809..8816
                     /locus_tag="VFH83_02900"
     CDS             7809..8816
                     /locus_tag="VFH83_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6485339.1"
                     /translation="MITIALVGGAHIHTPNFIQKLSGRPGVTVSRVWDHDAERARRRA
                     EPLRARVAREAAEIWDDPSIQAVVICSETDRHEPLVRAAAKAGKHLFVEKPLGMGARD
                     AAAMAEAIERAGVLFQTGYFLRGTPIHRFLRSQIQAGAFGKLTRYRQTNCHAGSLKGW
                     FDTEWRWMADPRQAGCGAFGDLGTHALDIILWMLGDPLSVTARIASATGRYGDCDEYG
                     EGMLQFANGVMGTLAAGWVDVAHPVPLILSGTEGHAYVANDQLYFQSGRVPGAEGQKP
                     WTDLPDALPHAFDLFLDAVEGKPATLVTAREAALRSAVMEALYTAARESRWVEMPPTA
                     R"
     gene            complement(8821..9321)
                     /locus_tag="VFH83_02905"
     CDS             complement(8821..9321)
                     /locus_tag="VFH83_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485340.1"
                     /translation="MGAETVPFHTIPPAPAAVSAGTILGRLADAIGFRFRWASEGLTD
                     ADLPYKPAPDCMSTGEQLDHICDLLATVSRNVGAPPLPPLAQPATCAPLRARILEISA
                     DLSARFKGMSDGDLSGRTPPLWNMVNGPMADCLTHIGQILSWRRLAGAPPAAADVFRG
                     LPPKGL"
     gene            complement(9335..11023)
                     /gene="ettA"
                     /locus_tag="VFH83_02910"
     CDS             complement(9335..11023)
                     /gene="ettA"
                     /locus_tag="VFH83_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HET6485341.1"
                     /translation="MPKDQEPNKIIYSMVGVNKSYDQKTVLKDISLSYFYGAKIGVIG
                     LNGSGKSTLLRIIAGVEKEFRGQIILAPGYSIGYLEQEPQLPPQSTVREVVEEGVQEV
                     VGLLKEFEEINTRLAEPMDDDAMTAILERQGEVQEKLEAAGGWDLDSRLEMAMDALRC
                     PPGDLKVSVLSGGERRRVALCRLLLQKPDILLLDEPTNHLDAETVAWLEHHLVQYEGT
                     VIAVTHDRYFLDNVAGWILELDRGEGIPWKGNYSGWLEQKGERLRREEKQESDRQKTL
                     QRELEWIREAPRARQAKSKARINSYESLLAQDSGNRMQELQIYIPAGPRLGDLVVECD
                     GLKKAYGDRILFEGMRFAVPPGATVGIIGPNGAGKTTLFRIITRQEKPDEGTMKVGET
                     VKLAFVDQLRESLDPKKSVWELISGGLDLMHVGKYEVNSRSYLARFAFSGSDQQKLVG
                     SLSGGERNRVHLALMLKEAANVVLLDEPTNDLDVNTLRALEEGILAFAGCVLVISHDR
                     WFLDRIATHILAFEGDSAAFWFEGNFSEYMENKRKRLGDAAVQPHRIKYRQLTR"
     gene            complement(11057..>11575)
                     /locus_tag="VFH83_02915"
     CDS             complement(11057..>11575)
                     /locus_tag="VFH83_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6485342.1"
                     /translation="TYDLVIANAFLDIVDYRAALPGLRDLARPGGRLLFSITFDGLTS
                     FEPEIDRELDDRIVATYHRTMDERFVDGRPFGDSRCGRHLFGALPTAGLRIAAAGASD
                     WVVVPVGGRYPADEAYFLGRMLAFFEEALSVRPEIGKAELRVWLERRRAQLGAAELCL
                     IVHQLDVLAQRP"
BASE COUNT         1901 a         3903 c         3845 g         1926 t
ORIGIN      
        1 gagccgccta tcctgggacg gtgaagaaca gaacctaagg agatgaaggt ctacttctac
       61 gatgccgggg ggaagggtgt cacagacata tcggcgctgg atctctcatc ggagaacgct
      121 gccgaggcag gttggggtgg cctcacgagg ttctcgggca acatagcgga cgtcgtctct
      181 gaactcagct ccgagggcta gtgggactgc atgcctccgc ggttccgaca gagcgtgttc
      241 agcgtcgcgg agctcgcgag cggattccgg cccgggcttc acgcttttga atgccgctga
      301 cgcaagacgg atcgtgtgct cgaacactcg gaagatcgct ggcagcgtca atgtcgaccg
      361 atgtctggct cgtttgatcc cggacgaacc gcgatgggac tacgccatcg gtcagtccgc
      421 gtccgctgcc gatattgtcc actgggtcga agtgcaccca tgcaacgcta gtgaggtcga
      481 acaggtgatc aggaagctgg cctggctgaa gacctgggtc ggggtccacg cgcgtaccga
      541 aatgagccag cgatacattt ggatcccatc gggaaaggcc gcgttgcccc cgacaacgcc
      601 acaatacaag cgggccgccg cgcacgggat caagatatcc ccgaggcagt gctatctgtt
      661 ctgattctcc ggcgtatgag tcgcctctct ttgaccagat gcccctcaac ggtatactcg
      721 aacccatgag cgacgcgaaa ccccgactgc tgatcgatcc ctctccgcgc acgatgcgcg
      781 agatcttcac cgaccaggac ctggcccggg tccgcgggct gtacgacgtg gtctggggtc
      841 gggacgaccc gatctcccag gaggccttcg accgcgagaa gagagacctg tttgccgtgg
      901 cctgctgcgg gtggcggtac ggttcgatcc aggaaatggc caggctgcgt gtcctgctgg
      961 acgtctgcgg cggcccgccg cggcccgacc aactcgacta cgcaagctgc ttcgagcggc
     1021 ggatcgccat cctgaactgc tctccggcgt tcggcccggt ggtggccgag atggcgctgg
     1081 ggatggcgct cgacgccgcc cgggagatct ctttcggcga ccgggagttc cgggccgctt
     1141 ctgagcacta cctccactcg gggaacgtgc ataccctcag cctcttcggc agcgcggttg
     1201 gcctcatcgg ctatgggagc ctggcgcgca acctccagcc cctgctcgcg ccgttccgat
     1261 gccgcatctc ggtctacgat ccctggaaga cggaggccca actccgcggc gccggcgtga
     1321 cccccgccac gctggatgag gtcctgcgag ggtccgacgt gatattcgtc ctggctcagc
     1381 ccacgaccgg caacaaggcc atgatcaccc gccggcacct ggagctgatc cagccccaca
     1441 aggtcttcgt gctcatcagc cgatcccacg tcgtggactt cgatgcgctc accgatctgg
     1501 cgctccagga ccggttcaag gccgccatcg atgtgttccc cgaggagccg ctggccaagg
     1561 atcacccgat ccgcaaggcg ccgggcgccg ttttgtcggc gcatcgggcg ggggcgcttc
     1621 ctgtcgcact cctcgatatc ggccgaatgg tggcggatga tctggaagcc atcatccgag
     1681 gactgccccc gtggcggatg cagcgcacgg agcccgagct ggcgcagaaa ctgtaggagg
     1741 tcgggtagaa atgaaggttc tcgtgattgg cgccaaaggc accatcggca aggccgttgt
     1801 ggatgccttg gagcgccgcg ggcatgaggt agtgcgggcc tcgcgcacct ccgatatccg
     1861 tgttgacctc gaggacccca agtcgatcca ggcgctcttc tccagcgtcg ggaaggtcga
     1921 cgcggtggtc ggctgtgccg gaagcggcgg atgggggccc ctggcaaccc tgtccgatca
     1981 ggacttcgcg ttctccctgc gcagcaagct gatggggcag gtgaacctga tccgtatcgc
     2041 ccagagccac gttcgggacg gcggctccat cacggtcacc agcggaaccc tggcgacgca
     2101 cccgcaaccc ggcggcgccg ccctatcgct cgtgaacgcc ggcctggatg ggttcgtgcg
     2161 cgccgcggcc ctggagctgc cccgtggcat ccgggtgaac gtggtgagcc cgccgtgggt
     2221 gaaggagacc ctgcgcgcca tgaaggccga cgagagccgc gggctggccg ccgcggacgt
     2281 tgccaaggcc tacctcgagg ccgtcgaagg caggggaaac ggcgaggtgc tcgagccgac
     2341 ccgcttcctc tgagcgggtc tgaccggttg gggcagcggc cccgtgctcc cggcgtctca
     2401 actcgttcca ctcgacgcaa ccttcgcctt ccagtcgatg gggcccgtcg tcacgtgccg
     2461 gtccaggatc tcccgctccg attcgccgat cgatgcggct gggatgtacg tcatgagccc
     2521 cagctcgtgc gtgacgtggt agaatcgcgg gttgcgtcgg cctatgtgca ggttctcgaa
     2581 gggcatgtgg aaggagctct tgtcgtattc gagctccagt cccgtttcca ggacccggat
     2641 ccggtagggc accgtcgaca gccgcgtggc ccgcgcctgc acggttcccc ggcgccctcg
     2701 ggtcaccagc agcgtcgcaa tggtcagaaa cagaaaacca ccggccagga cggcagcccc
     2761 tacgtactgt cgtagggcca cctgtaccgc gaccaggccg acggccacga tcacgggcag
     2821 ggccggagcc agccgcgccc acaccgtctt tctgccgcgc gggtaaagga cactcaacga
     2881 cgcttcccgg tagcggtcct cgccaagggt gaactcgttc atggggatcg actcccttcc
     2941 gatggcggcg gcgacgcgga ggatgaggtt ccagtccacg gcgtggctcg tcgcggctat
     3001 ctggacctgg cggatcgcgt ccacaacccc ctgcaggacc tggatccggc cgccaagctc
     3061 ctgactctga cggtcgagga tgccggacag gtccgccgtt tccgtgtcga ggatccgccg
     3121 gatctccgcc aacggcagcc cgagggcctt cagggcaagg atccgacgga ggcgctcgaa
     3181 gtcccgccaa tcgtagagtc ggaatccggc gagggaacgg cgacgtgaga ccagaagccc
     3241 ttccaggtcg tagaagcgga ccgtacgcac ggagaccctc gccttcctgg cgaagatccc
     3301 gatgcggtag agcctctcgt cctcagcgtt tgcgctcatc acctccgaac ctaccccctg
     3361 acgttgcgtc agggtcaagc gtttttttcc ctgccaccct acctcgtgtg tcccgcctca
     3421 ccgcttatgc caggcccgcc ccgcctccat gaagacccga aggttctcct cgggtgtctc
     3481 ctgcggcaga tcacagcccg tgctcagcac gaagtttggg aaccgatcca tctggcggag
     3541 gagctcctcc acgtcccgct gaacgtccga agggctcccg aaccgcatcg tggtggtagg
     3601 gctgatattg ccgatcacca cgacgtcctc ggggacctgc ttcgaaagcg ttggaaggtc
     3661 cacccctgcc tcccgggagt ccaggctcag cccgttgaca ccggaccgca ccatcttctc
     3721 gaccaggtgc gtcgtgttcc cacaggtatg gtagacgaag ttgatcccgc tgtatttgaa
     3781 gctggtcgtg atatgccgga tgtagatcgc ggagaactcc gagaactgct gggggctcaa
     3841 catcacggcg ctgggctcca ggatgcagat gacctgcgcc cccgcggaga tcagcatctg
     3901 cacgtactcc tggatgcgct cggtggtgaa gtggcacagg cgatggagca ggtccggctc
     3961 cgtcaccgtt gccatcgcgg cctcgtccgc ccccatcatg aggccggcga gcgtgaaggg
     4021 ccccaccgcg tagcctcccc ggaggatcgc cggtgggagc gtgacgctca tcatccgcat
     4081 catctcgaca tagcccagga cccggctgtc gaagctgatg ttgatccttc ccaggcgttc
     4141 cagctcgtcg atgctgaagc gatccttggg caccgtggcg gaatcctcct gcggaaagac
     4201 cgtgtagcgc cccagcgcgt tggcctccac cgcgagatcc atgagcggga acgccacgtc
     4261 cggctggaaa cggtccacca gggcgcggat ggcccggtag tgctctccgt agttctgctg
     4321 ggccagcttg atcgtgcttc cgacgatttc cacgccgggg aacccggcca gcggcgccac
     4381 caaccgtcgg ccatgatcga acgcctcgtg ggtcagatcg gcgaggctct ttccctgcat
     4441 cacggatcaa cgatagccga tcggggcggc cctgcaaagg gcgccggtac cgctctgcgc
     4501 tgccgcggaa tcacgccgtg gaggattggt agcgcgcgag ctcctcgggg agcaggatga
     4561 ggaccgcgcc gcgggagact cggaggatgc cgcgcgactc gaagtccttg aggatgcggc
     4621 tgacgacctc acgggaggtt ccgatctcca gggcgatgcg ggcgtgggtg gtccggacgg
     4681 ctccgacgcg ggagggcgcc gacagaagat agccggcgat ccgctggtcc acgtgccgga
     4741 aggccacctc ctccaccagg gcgagcatcg agtccagcct ccgagcgtac agcccgaaca
     4801 cgaacgcgcg ccacgcgggg tgcttctcca cgagacccga gaggagccgg gccgggagaa
     4861 gcgctaccag cgtcggaccc tcggcgcggg cgatggcggg gaacccgtcg tggttcatga
     4921 tgcaggtcgc cgtgagcacg caactctccc cgcgctcgat gcggtagagc gtgagctccc
     4981 gtccctcgct gctcaccttg tagacccgca gggtgccctc cagcaccagc gggaggtagg
     5041 cgcattccga gccgccgctg accaggatct ggtccggctc cagccgcttg gcgatcgcct
     5101 gggacaggaa ctgctcgcgg gatgccgagg gcacggagtc caggaacggg aaggcggaca
     5161 gcgccactcc tctgtcgatc atgtcggacc tcgcggccca ggatagtcca ccgagggtga
     5221 gatggatagc gctccggatt gccgatgcgc gatgcgtcac cgtgacatgg ctctgcgcct
     5281 gccggatcag gcggctcggc cgggcgctcc gttgggaccg tcgctcgggt ccccgaggta
     5341 gacctcagag catccctgcg atagcctcag ggacccaggg gttaccgatc aatcgacgtt
     5401 gcggacgggg gagcgaaatg aggtacgcgt gcgcaatcga cttcggaacg accaactccg
     5461 tggtcgccct cgcccgagag ggtgagccga agccccaggt ggtggcctcg gaaccctcgt
     5521 gcatcctgat cacggatgac cgcagcaccc agcggctgta cgcgggcaac gacgccattg
     5581 cgcactaccg aggcgccgcg gacaccacgc ggttcgtgaa atccatgaaa agcctgctct
     5641 cggatccggg gttccagagc acgcgcgtgt tcggtcgaac ctacaccgcc gcgcacctgg
     5701 tgcgcccggt gctgagtcac ctcaaggcgc tggccgaggg ggcgttgggc gaaaggatcc
     5761 accgggtgat cctggggagg ccggtgcact tcagcgcagc gtgggaacac gacgacctgg
     5821 ctgagggtcg gctccgggag gcggcggtcc tggccggctt cacggagatc acgttccaga
     5881 aggaaccgat tgccgcgggg tggagcttcg cctcgaccct tcaggaggag cgcagagtcc
     5941 tcgtggcgga cctgggcggc ggcacggcgg acttctgcct ggtggtcttc cggccggatg
     6001 ggcggcacca ggtgttggcg gtcggcggcg cgcggatcgg aggggacgac ttcgacggcc
     6061 gcatcatgtg gggccggctg atctccagct tcggctacgg cagcacatac gagtcctggg
     6121 gcaagatgct ggaggtcccg atccacatct accgggcgct gtgtcggtgg gaccggatcc
     6181 cgttcctcaa ggagtccagg acctggcggg acctccggta catccaccag ggatccacgg
     6241 acaagcccgg catcgcgcgt ctcatggcgc tgatccgaaa cgacctgggc ttcccgctgt
     6301 accagcgcat cgccgccgcc aaacacgagc tctcccgggc ggtcgtctct accattcgga
     6361 tggaccagga cgaggtcacc ctccgcgaag agttgtcccg cgcccagttc gagtcgatga
     6421 tcgcggcgga tgtggatcag atcctcggca cggcatcgga gaccctgacg accgccggaa
     6481 tgaaaagcac ggacgtcgac atttgcttcc tgacgggcgg ctgttccctg gtccccgtcg
     6541 tggcatcccg gttcgcccgg ctcttcccgg gcggccggct ctccaccgag ggggacgcat
     6601 tcaccagcgt ggcatcggga ctggccatct atgggcttac ggcttgagcg gctgccccgg
     6661 cggccacggc tctcacaccc gccgtcaccc ccgctacggg atgagaacga tcttgccgtg
     6721 ggcgccgggc tccaggatcc ggcggtgcgc ctgtgcggcc tcggcaaggg gcagctcgac
     6781 accgaccacg ggctgaagcg tcccctgctc caggccctcc cccagggccg catacgtgct
     6841 gagcgcatcc gcgtcggaca ggttccagag aagcatcccg tgcaccgatg cgtcccgccc
     6901 catgaggtcc cgcggcgtga tggtcacatc tccccggctt ccgatgatca ccacccgccc
     6961 cttcggggcc aggagcttca ggtccatgcc caggttcacg ttggcgagca tctccaggat
     7021 cacgtcgacc ccgcgcccgc cggcgagctt catgatctgc tcagcctgat ccgcggcgcg
     7081 atgatccacc gcgtgccgcg cgccctcgcg caggatgagc gcgcgccccg catcggtccc
     7141 ggcggtcccg atggccgtca acgcgaggtt tcgcgaaagc tgacacgcgg cgatcccgac
     7201 cccgccgctt gcgccgtgga tgagcacggt ctccccggcg cgggcgtgcg ccacgtgcgc
     7261 gagggcgtga aacgcggtgg cgtacggaat gttgattccg gctccttgag aaaagctcac
     7321 gcgctcgggg agggcgtgaa gctgggactc ccgcaccagg accatctcgg cgtaggagcc
     7381 gctgagggtg ccggccgtat acacgcgatc tccgggcctc catccgtgga cgtcgcttcc
     7441 cactctctcc accacgccgg ccgcgtccga gcccggagtg tagggaggct tggcggtccg
     7501 caccatgccg ctgcgctggt aggtctccgt ggggttcacc ccgacggcct ttacgcgaat
     7561 cagcacctgg ccggcgcccg gcaacggctc cgccgcctcc tcgagcttca ggacctcagg
     7621 acccccgaac tcccgtacca cgatcgcttt catttccatt tcctcgctgc aacgaatgta
     7681 aggggcaaag cgagcccgcg ccaaacgggc gccgccttga ccccggcggc gtccggcggg
     7741 agaataccgc cgtgaagccc agcgaggctg ccggcaacga agtccctatc ccgtcctgag
     7801 gaggcaccgt gatcaccatc gccctggtag gtggcgcgca catccacacg ccgaacttca
     7861 tccagaagct gagcggacgc ccgggagtaa ccgtttcccg agtctgggac catgacgcgg
     7921 agcgggcacg gcgccgagcg gagccgcttc gagcgcgcgt agcgcgagag gccgccgaga
     7981 tctgggacga cccgtcgatc caggcggtgg tcatctgctc cgagacggac cgccacgagc
     8041 cgctggtccg ggccgcggca aaggccggca agcacctgtt cgtcgagaag ccgttgggca
     8101 tgggcgcccg ggacgccgcc gccatggcgg aggccatcga acgagccggc gtcctcttcc
     8161 agaccggcta cttccttcgc ggcaccccca tccaccgctt cctccgctcc cagatccagg
     8221 ccggcgcgtt cggcaagctg acccgctacc ggcagaccaa ttgtcacgcg ggctctctga
     8281 agggatggtt cgacacggag tggcgctgga tggcggaccc gcggcaggcg ggctgcgggg
     8341 cgttcggaga cctggggacc catgccctgg acatcatact ctggatgctg ggggatccgc
     8401 tcagcgtcac ggcgcggatt gcttcggcca cgggccgcta cggggattgc gatgagtacg
     8461 gggaggggat gctgcagttc gcgaacggtg tgatggggac cctggcggcc gggtgggtgg
     8521 atgtagcgca tcccgttccg ctgatcctga gcgggacgga agggcacgca tacgtggcga
     8581 acgaccagct ctacttccag agcgggcggg tgcccggcgc ggaaggccag aagccctgga
     8641 ccgatctgcc cgatgccctg ccgcacgcct ttgacctgtt cctggacgcc gtggagggaa
     8701 agccggccac cttggtcacg gcgcgcgagg cggcgcttcg aagcgcggtg atggaggcgc
     8761 tgtacacggc ggcaagagag agccgctggg tggagatgcc ccctacagcg cgatagagcg
     8821 ctatagaccc ttcggcggca gcccgcggaa gacgtccgcg gccgcgggcg gcgctccggc
     8881 cagccggcgc caggagagga tctggccgat gtgcgtcagg cagtccgcca tcggcccgtt
     8941 gaccatgttc cacaggggcg gggtgcggcc gctgagatcc ccgtcggaca tgcccttgaa
     9001 ccgggcagac aggtccgccg agatctccag gatgcgtgct cggagcgggg cgcaggtggc
     9061 cggctgcgcc agcggcggca gcggcggcgc cccgacgttc ctggacacgg tggccagaag
     9121 atcgcagatg tggtcgagct gctctcccgt gctcatgcag tccggcgccg gcttgtaggg
     9181 cagatccgcg tccgtgaggc cttccgatgc ccagcggaac ctgaacccga tggcatccgc
     9241 cagccggccc aggatggtcc ccgcgctgac ggcggccggc gccgggggga tggtatggaa
     9301 gggaaccgtt tccgcgccca tgggagcgct cctttcagcg cgtgagctgc cggtacttga
     9361 tccggtgcgg ctgtaccgcg gcatcgccca ggcgctttct cttgttctcc atgtattccg
     9421 aaaagttgcc ttcgaaccag aaggcggcgc tgtctccctc aaaggccagg atgtgcgtgg
     9481 cgatccggtc caggaaccag cggtcgtggc tgatcacgag cacgcacccc gcgaaggcca
     9541 ggatcccctc ctccagggcg cgcagcgtat tgacgtccag gtcgttcgtc ggttcgtcca
     9601 ggaggaccac gttcgcggcc tccttgagca tgagcgccag gtgcacgcgg tttcgctccc
     9661 ctccgctcaa ggagcccacc agcttctgct gatcggagcc gctgaacgcg aaccgggcca
     9721 ggtaggatcg ggagttcacc tcgtatttcc cgacgtgcat caggtccaga ccgccggaga
     9781 tgagctccca cacgctcttc ttgggatcca gactctcgcg aagctggtcc acgaaggcga
     9841 gcttcacggt ctcgccgacc ttcatcgtcc cctcgtccgg tttctcctgc cgggtgatga
     9901 tccgaaagag ggtggtcttc cccgcgccgt tgggaccgat gatcccgacg gtggcccccg
     9961 gtggaacggc gaaccgcatc ccctcgaaga ggatccgatc gccgtaggcc ttcttcaggc
    10021 cgtcgcattc cacgacgagg tcccccagtc gcggaccggc ggggatgtag atctgcagct
    10081 cctgcatccg gttgccggag tcctgggcca gaagagactc gtaggagttg atgcgcgcct
    10141 tgctcttggc ctgtcgggcc ctcggcgcct cgcggatcca ttccagctcc cgctgcagcg
    10201 tcttctgccg gtcggactcc tgcttctcct cccgccgcag gcgctcgccc ttctgctcga
    10261 gccagcccga gtagttcccc ttccagggga tcccttcccc gcggtccagc tccaggatcc
    10321 atccggccac gttgtccagg aagtaccgat cgtgggtcac cgcgatgacc gtgccctcgt
    10381 actggaccag gtggtgctcg agccacgcca cggtctccgc atccaggtgg ttcgtcggct
    10441 catccagaag caggatatcc ggcttctgaa gcaggagccg gcacagcgcc acgcgccgcc
    10501 tctctccgcc cgagaggacg ctcaccttca ggtcccccgg agggcagcgc agcgcgtcca
    10561 tcgccatctc cagccggctg tccaggtccc agccgcccgc ggcttccagc ttctcctgga
    10621 cctcgccctg ccgttccaga atggcagtca tggcgtcgtc atccatgggc tcggcgagcc
    10681 tcgtgttgat ctcctcgaac tccttgagga gcccgacgac ctcctggacc ccttcctcca
    10741 ccacctcgcg gacggtgctc tgcggaggga gctgcggctc ctgctccagg tacccgatgg
    10801 aatagccggg cgccaggatg atctggccgc ggaactcctt ctccacgccg gcgatgatcc
    10861 gtagcagcgt gctcttgccg gatccgttca ggccgatgac gccgatcttg gcgccataga
    10921 agtaggaaag tgagatgtcc ttgaggacgg tcttctggtc gtagctcttg ttgaccccga
    10981 ccatggagta gatgatcttg ttgggttcct ggtctttcgg cacgggggcc agcctaaggg
    11041 gagtggggtg ccgagatcaa ggccgttggg caaggacgtc gagctgatgc acgatcaggc
    11101 acaactcggc cgccccaagc tgcgcgcgcc gtcgttccag ccagacccgg agctccgcct
    11161 tcccgatctc gggccgaacg gacagcgctt cctcgaagaa cgccagcatg cggcccagga
    11221 agtaggcctc atccgccggg tagcggccgc ccacgggcac caccacccaa tccgaggcgc
    11281 cggccgcggc aatccgcagc ccggccgtcg gaagggcgcc gaacaggtgc cgcccgcacc
    11341 gactatcgcc gaacggccgt ccgtcgacga agcgctcgtc catggtgcgg tggtacgtgg
    11401 ccacgatccg gtcgtccagc tcgcgatcga tctccggctc gaaggaggtc agtccgtcga
    11461 aagtgatgga gaagaggagc cgtccgccgg gtcgggcgag gtcccggagc ccgggaagcg
    11521 cggcgcggta gtcgacgatg tccagaaagg cattggcgat gaccaggtcg taggt
//
LOCUS       DASRZS010000121         3662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5068124, whole genome shotgun sequence.
ACCESSION   DASRZS010000121 DASRZS010000000
VERSION     DASRZS010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5068124"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VFH83_02920"
     CDS             complement(<1..762)
                     /locus_tag="VFH83_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6485343.1"
                     /translation="MQRTRRVLLSVICAFLVAALPCLALGGREPAVAASPAHPLTFVM
                     VPKGVHPYFEPVYRGFEEAARKYGVVSEVDAPPRFDVALQVKVIEDLIARGVSGIAIS
                     ANDDQGLVEVVHEATQAGIKVITVDAPAPSTEALTYIGTDNESAGYEAGRRMAKEMGG
                     KGSVAILQGGLAATNLNLRTLGFKNALARQAPGIQVVEVADEGGDFALSVSKTEDLLS
                     RYPSLSAIFSVSAEGAPAAAAVLKQRGRSGRVLVAG"
     gene            complement(988..2301)
                     /locus_tag="VFH83_02925"
     CDS             complement(988..2301)
                     /locus_tag="VFH83_02925"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6485344.1"
                     /translation="MRSRGDFSILIVDDEEGMRLGLEKTLSLEGYRVASAATGAEARS
                     AARDNRFDCAFVDLKLPDLSGTDLLSDLREAGTAVVVITAFASVDTAVRAMKLGAVDY
                     LQKPFDNQDIVALADRLCVGPARTAHAETESISGEDFVAASPAMRQILTTLRKVMNSE
                     IPVLLQGESGTGKEMLARFIHQKGDRAGKPFAAINCAAIPPELLESELFGHEKGSFTG
                     AHATVAGKFEAAGAGTIFLDEIGELSPHLQTKLLRALEERSFEPVGSTRPVPLAARII
                     ASTNSDLAAMIREKLFRLDLYYRLKGILVTIPPLRERREDIEPLLGIFLELFRQRYRK
                     PGVEVSREAIRFLQSYQWPGNVRELKNAIESAVLLSEQSRPLLPQDFLLEGAIETPLP
                     ELWKQERETIIEVLRRTGYNRSIAARELGMSRKTLYNKMKRYSIR"
     gene            complement(2298..>3662)
                     /locus_tag="VFH83_02930"
     CDS             complement(2298..>3662)
                     /locus_tag="VFH83_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6485345.1"
                     /translation="RDTIPRDLPGRLSGRPDYLQTTDGIYATFYMPVFRAGDEIGLLV
                     LQKAFNRGYLETLSLRFSIGIALYTQGLYRYSSLPGIEPAGVLWARARPMTDRPFSGT
                     YSYQGQTYRYLGSYFEMGPLAKGFLIVGGASSITAAGWWRNFTGLSIIPLICVAAAIA
                     LFFFWGSEVIRAIRLLLAASLAVGRGNYRVHLPVERRDEFGELYRGFTRMAEQLEENL
                     ARLEESKRLLVTNERMAAVGRFSAGVAHEINNPLGIVLNHVQLLRSGRLADSETEAFL
                     ERMETEIKRMNRLLRTLLHHATDEELSFSDVRLDTVVGEVVQLFTPKLRLKGVRVEVA
                     AFPDRCMVEGDADAIKQVFFNLLYNAIQAIHHDHGLVSIEACPQDGGFRVSVRDNGVG
                     MDESTRASIFQPFVTRKQGYGTGLGLALSQTILKQHGGTISVAGELDVGTTVTLWFPR
                     KEEA"
BASE COUNT          592 a         1227 c         1236 g          607 t
ORIGIN      
        1 ccccgcgacc aggacccggc ccgacctgcc gcgctgcttg agcaccgcgg ccgctgcggg
       61 cgctccttcg gcggacacgc tgaaaatggc gctcagggac gggtatcggg agaggaggtc
      121 ctccgtcttg ctcacggaaa gggcgaagtc tccgccctcg tcggctacct ccaccacctg
      181 gatgcccggc gcctgccggg cgagcgcgtt cttgaagcca agcgtccgca ggttgaggtt
      241 cgtagccgca aggcccccct ggaggatggc aaccgagccc ttgccgccca tctccttggc
      301 catccgccgg ccggcctcgt agccggcgct ctcgttgtcc gttccgatgt aggtgagcgc
      361 ctcggtggag ggcgccggag cgtccaccgt gatcaccttg attccggcct gcgtcgcctc
      421 gtggaccact tccaccaggc cctgatcgtc gttcgcggag atggcgatcc cgctcacccc
      481 gcgagcgatc aggtcctcga tgaccttgac ctggagcgct acgtcgaacc gcggcggcgc
      541 gtccacctcc gacacgaccc cgtacttacg ggcggcctcc tcgaatcccc ggtacaccgg
      601 ctcgaagtac gggtgcacgc ccttggggac catgacgaag gtgagcgggt gcgcggggga
      661 cgcggcaacc gccggctccc gaccgcccaa cgcaaggcag gggagagccg cgaccagaaa
      721 cgcgcagatc acactgagga ggactcgccg cgtgcgttgc accgttttcc cgcgagctcc
      781 ggtcatgcag gaagcgggcc aatcccgcgt ctcggcgctg aaggtatatc ccgtgcgggc
      841 ataaggactt gcggcctggc ccgaacgaag cctccgcccg aacgtggctg tgagtcctgg
      901 cgttgcccga tttgggtaag aatgggtaac cgcgagcccg gcccggtctc cccctgcggg
      961 ccatgcgacg gccgatccca gcggagttca gcggatcgag tagcgtttca tcttgttgta
     1021 cagggtcttg cggctcatcc cgagctccct ggcggcgatg gaccggttgt acccggtgcg
     1081 ccgaagaacc tcgatgatgg tctctcgctc ctgcttccag agctccggca ggggagtctc
     1141 gatggcgccc tccagaagga agtcctgcgg caggaggggc cggctctgct ccgaaaggag
     1201 gaccgcggac tcgatggcgt tcttcagctc ccgcacgttg ccgggccact ggtaactctg
     1261 aaggaaccgt atggcctcgc gggagacttc cacgccgggc ttccgatagc gctggcggaa
     1321 gagctcgagg aagatcccca gcaggggctc gatgtcctcc cgtctctcgc gcaagggcgg
     1381 gatcgtcacc aggatcccct tgagtcgata gtagaggtcc aggcggaaga gcttctcgcg
     1441 gatcatggcc gccaggtcgc tgttggtgga ggcgatgatc cgggcagcca acggcacggg
     1501 acgggtggac ccgacgggct cgaaggagcg ctcctccagg gctcgaagga gcttggtctg
     1561 cagatggggg ctgagctcgc cgatctcgtc caggaagatg gtaccggcgc cagcggcctc
     1621 gaacttaccg gccaccgttg catgggcacc ggtgaaggag cccttctcgt ggccgaagag
     1681 ctcgctctcc aggagctcgg gagggatggc ggcgcagttg atggcggcaa aaggtttgcc
     1741 ggcgcggtcg cccttctggt ggatgaaccg ggccagcatc tccttgccgg tcccgctctc
     1801 tccctggagc aggacgggga tctcggagtt catgaccttg cggagcgtgg tgaggatctg
     1861 ccgcatggcc gggctggcgg ctacgaagtc ctctccggag atggactccg tctccgcgtg
     1921 ggcggtacgg gccggcccca cgcacaaccg gtccgcgagg gcgacgatgt cctggttgtc
     1981 gaacggcttc tgtaggtagt ccaccgcgcc cagcttcatg gcccgcaccg ccgtgtccac
     2041 gctggcgaag gcggtgatca ccaccacggc ggtccccgct tccctcaggt ccgataggag
     2101 gtcggtgccg ctcaggtccg gaagcttgag gtccacgaac gcgcaatcga atcggttgtc
     2161 ccgggccgcg ctccgtgcct cggcgccggt tgcggcgctt gccacccggt agccctccag
     2221 ggagagggtt ttctccagtc ccaggcgcat gccctcctcg tcgtcgacga tcaggatcga
     2281 gaagtctccg cggctcctca cgcctcctcc tttcggggga accagagggt gaccgtggtc
     2341 ccgacgtcca gctctccggc tacggagatc gtcccgccgt gctgcttgag gatggtctgg
     2401 ctcagcgcca gccccaggcc cgtgccgtag ccctgcttgc gcgtaacgaa gggctggaag
     2461 atcgaggccc gcgtggactc gtccatgccc acgccgttgt ccctcacgct cacgcggaag
     2521 ccgccgtcct gggggcaggc ctcaatgctc accaggccgt ggtcgtggtg gatcgcctgg
     2581 atggcgttgt acagcaggtt gaagaagacc tgcttgatgg catccgcgtc tccctccacc
     2641 atgcatcgat ccgggaaggc cgccacctcc acgcggacgc ccttcaggcg gagcttgggc
     2701 gtgaagagct gcaccacctc ccctaccacc gtgtccaggc gcacgtcgct gaaggacagc
     2761 tcctcgtccg tggcgtggtg gaggagcgtc ctcaggagcc ggttcatccg tttgatctcc
     2821 gtctccatgc gctccaggaa cgcctcggtc tccgagtccg ccagcctgcc ggacctcagg
     2881 agctggacgt ggttcagaac gatccccagg ggattgttga tctcgtgcgc caccccggcg
     2941 gaaaaccggc ccaccgctgc catccgctcg ttggtgacca gaaggcgctt gctctcctcc
     3001 agccgcgcca ggttctcctc gagctgctcc gccatgcggg tgaagccccg gtacagctcg
     3061 ccgaactcgt cccgcctctc cacggggagg tgcacgcgat agttcccacg ccccaccgcc
     3121 agagaggcgg ccaggagcag gcggatggct cggatcacct cgctccccca gaagaagaac
     3181 agggcgatgg cggcggccac gcagatgagc gggatgatgg agagtcccgt gaagttgcgc
     3241 caccagccgg ccgcggtgat ggaagaggcg ccgcccacga tgaggaaccc cttggcgagc
     3301 ggacccatct cgaaatagga gcccaggtag cggtacgtct gaccctggta cgagtaggtg
     3361 ccggagaacg ggcggtccgt catggggcgg gcccgggccc acaggacccc cgcgggctcg
     3421 atccccggca gcgacgagta ccggtacaac ccctgggtgt agagggcgat cccgatggag
     3481 aagcgaaggg aaagggtttc caggtagccc cgattgaacg ccttctgcag caccaggagc
     3541 ccgatctcgt cacccgcgcg gaagaccggc atgtagaaag tggcgtagat cccgtcagtg
     3601 gtctgcaggt aatcggggcg acccgagagc cggccgggca ggtcgcgggg gatcgtgtcg
     3661 cg
//
LOCUS       DASRZS010000122         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5454303, whole genome shotgun sequence.
ACCESSION   DASRZS010000122 DASRZS010000000
VERSION     DASRZS010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5454303"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFH83_02935"
     CDS             complement(<1..184)
                     /locus_tag="VFH83_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485346.1"
                     /translation="MARTRRIHVNGDGWSTALLFTPGFVVYLVFMILPILLCLYYAFF
                     DWDGIRETMSFVGLDNF"
     gene            complement(255..1508)
                     /locus_tag="VFH83_02940"
     CDS             complement(255..1508)
                     /locus_tag="VFH83_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6485347.1"
                     /translation="MKRALLVLLILAAAATFTFAQVQKISWQVWVTPNLTREFYDGIV
                     SAFQAKNPNIQVTIIEANANVTPKADDFFKTRLAAGDVPDVVSNLTIPFFADAGLLWQ
                     IPANDPDLKAVPNLQAAAYKGKLYGFNSSVQPQGLMFYNKKLFAQVGITSVPKTWAEF
                     DADCAKLQAAGITPILAGGQWVAGYNFTIFTSPEIYHNDLNWYGDRWAGKVHFTDANY
                     VEAANWFKSLVDKGYFNPGALSIDYSSLQDHFLQGEEAIYPMGSWFTAAEASAQKDFD
                     VGVFFSPTKDGKPHLLQSLSYGTTPAIYAKSKYPDAAWKLVKFAIMDSNFGAKFIQAD
                     GLYSALKPPLTYPMSPLQNALGSLIQTPATTSALYNLKVGDMPPDGITDIYDKVGQAI
                     LAGGVTDVKPLMQQLDDFWNQAKHP"
     gene            complement(1681..2373)
                     /locus_tag="VFH83_02945"
     CDS             complement(1681..2373)
                     /locus_tag="VFH83_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6485348.1"
                     /translation="MRRAAGVDKIRVVIADDQSLITQSFKVLLETVARDIVVVGTAKD
                     GAEAVSVVEREKPDVVLMDVRMPVMDGVQATRLIHERQPESRIIMLTTFDDDTYVHEA
                     IRGGAVAYLLKDISTEELLSSIRAARLGTIMVSPTVAQKLVKPDGQGPVPEQPAEKLL
                     NELSRRETEILRLLVQGLENKEIAERLHAAEQTIKNAVSVIYQKLGVESRKEARRLAL
                     RSGLVRADDALS"
     gene            complement(2370..>3323)
                     /locus_tag="VFH83_02950"
     CDS             complement(2370..>3323)
                     /locus_tag="VFH83_02950"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6485349.1"
                     /translation="NYINVRTTLLASLVFETMVYGKTPVSYGFSLALIAACLFIRRPA
                     PLWDQRIEGISLDNLMFMGFYPLIVMVLGGLLKNAQRLASDRKRLVEQLRKASTSLVE
                     TNIQLQEHVVRSEEQAKLLERERISRELHDTIGYTLMNIIATMKASMELSRTDTARML
                     DFMGQGIEQAQKGLAETRAALRTLRTASSQRLSLVTTLNRLVSAFENTHIVVTASYSN
                     IPWFFGEEIDSTIYRIVQEGITNAIRHGNATVISIHVSWDGERISVTVTDNGSGAKEV
                     AEGIGMKGIRERLMQVSGGVSAGNAFGGFQLYAWIPMKRSA"
BASE COUNT          559 a         1016 c         1104 g          644 t
ORIGIN      
        1 caaagttgtc cagccccacg aagctcatcg tctcacggat cccgtcccag tcgaagaacg
       61 cgtagtacag acagaggaga atgggaagga tcatgaagac caggtagacc acgaaccccg
      121 gcgtgaacag cagcgccgtg ctccatccgt ctccgtttac gtgtatgcgt ctggtgcgcg
      181 ccatggtggc ctcgagggga aaggcacatg gagggccatg tgcctttccg ttgcttcgtt
      241 tgcttcggaa tgaactaggg gtgcttggcc tggttccaga aatcgtcgag ctgctgcatc
      301 aggggcttca cgtccgtgac gccgccggcg aggatggcct ggccgacctt gtcgtagatg
      361 tccgtgatgc cgtcgggcgg catgtcgccc accttcaggt tgtacagcgc ggaggtcgtc
      421 gccggggtct ggatgagact gcccagcgcg ttctgcagcg gggacatggg gtaggtcaag
      481 ggcggcttca gggcggagta cagcccgtcc gcctggatga acttggcgcc gaagttggag
      541 tccatgatgg cgaacttgac cagcttccag gcggcgtcgg gatacttgct cttggcgtag
      601 atggccggcg tcgttccgta ggacaggctt tgcagcaggt gcggcttccc gtccttggtc
      661 ggagagaaga acacgcctac gtcgaagtcc ttctgggccg aagcctccgc cgcggtaaac
      721 cagctcccca tggggtagat ggcctcttcg ccctggagga agtggtcctg gaggctggaa
      781 tagtcgatgg agagtgcgcc ggggttgaag tagcccttgt ccaccaggct cttgaaccag
      841 ttggccgctt ccacgtagtt ggcgtccgtg aagtggacct tgcccgccca gcggtcgccg
      901 taccagttca gatcgttgtg gtagatctcg gggctggtga agatggtgaa gttgtaaccg
      961 gccacccact ggccaccggc aaggatcggg gtgatcccgg ccgcctgcag cttggcgcaa
     1021 tcagcgtcga actcggccca cgtcttgggg acgctggtga tgccgacctg ggcgaagagc
     1081 ttcttgttgt agaacatcag accctggggc tggacgctgg agttgaagcc gtacagtttg
     1141 cctttgtagg cagccgcctg gagattcggg accgccttca ggtcgggatc gttggccgga
     1201 atctgccaga gcagcccggc atccgcgaag aacgggatcg tcaggttgga caccacgtcc
     1261 ggtacgtcgc cggcggccag gcgggtcttg aagaagtcat ccgccttggg cgtgacattc
     1321 gcgttggcct cgatgatggt gacctggatg ttcgggttct tggcctggaa cgccgacacg
     1381 atgccgtcgt agaactcccg ggtcaggttg ggggtcaccc agacctgcca ggagatcttc
     1441 tggacctgag cgaacgtgaa cgtcgccgcg gctgccagga tcagaagcac cagtagggct
     1501 cgtttcatac actctccttt tctgcgtgcg atcgcatggg gcggcgcaac gcgccggttg
     1561 cccgcaagaa ggacgggatc gtccccccta agaggagatc acaccacggg cgagggcgcg
     1621 gcgcaaggac gcagggtacc tggcgagtac cgattccgta ccgaaaaggt accgggggcg
     1681 ctagctcaga gcgtcgtcgg cccggaccag gcccgacctc aacgccagcc ggcgcgcttc
     1741 cttgcggctc tctacgccca gtttctggta gatcacggag accgcgttct tgatggtctg
     1801 ctcggccgcg tggaggcgct cggctatctc cttgttctcc aggccctgaa cgaggagacg
     1861 gaggatctcg gtctccctgc gggaaagctc gttcaagagc ttctcggcgg gttgctccgg
     1921 caccgggccc tgcccgtccg gcttcaccag cttctgggcc acggtagggg agaccataat
     1981 ggtgcccagg cgggcggcgc ggatggagct caggagctcc tccgtagaga tgtccttcaa
     2041 cagataggcc accgctccgc cgcggatggc ctcgtgcacg taggtatcgt cgtcgaaggt
     2101 ggtgagcatg atgatccggg actccggctg ccgctcgtgg atgagtcggg tggcctggac
     2161 cccgtccatg accggcatgc gcacgtccat gagcaccacg tccggcttct cccgctccac
     2221 aacggacacg gcttcggcgc cgtccttggc ggtccccacc accacgatgt ccctggccac
     2281 cgtttccagg aggaccttga agctctgggt gatgaggctt tgatcgtccg cgatcacgac
     2341 ccggatcttg tccacgcctg ccgcccgcct cacgcgctcc gcttcatggg aatccaggcg
     2401 tagagctgga acccgccgaa ggcgttcccc gcgctgacgc cgccggacac ctgcatgagg
     2461 cgctcgcgga tccccttcat gccgatgccc tccgccacct ccttggcccc gctcccgttg
     2521 tccgtgacgg tgacgctgat cctctccccg tcccaggaca cgtggatgga gatgacggtg
     2581 gcgttcccgt ggcggatggc gttcgtgatc ccctcctgga cgatgcggta gatcgtggag
     2641 tcgatctctt ccccgaagaa ccaggggatg ttgctgtacg aggccgtcac cacgatgtgg
     2701 gtgttctcga aggcggagac cagacggttg agcgtcgtca ccagggacag ccgctggctg
     2761 gaggcggtgc gcagcgttcg aagcgcggcc cgtgtctccg ccaggccctt ctgcgcctgc
     2821 tcgatcccct gtcccatgaa gtccaacatg cgggcggtgt cggtccggga gagctccatg
     2881 gaggccttca tggtggcgat gatgttcatg agggtatacc cgatggtgtc gtgcagctcg
     2941 cgagagatgc gctcccgttc caggagcttg gcctgctcct cgctgcggac cacgtgctcc
     3001 tggagctgga tgttggtctc caccaggctc gtgctggcct tgcgaagctg ctcgaccaga
     3061 cgcttgcggt ccgaggccag ccgctgggcg ttcttcagaa gaccgcccag caccatcacg
     3121 atcagggggt agaaccccat gaacatcagg ttgtccaggg agatcccctc gatcctctgg
     3181 tcccagagcg gcgcgggcct tcgaatgaag aggcaggcgg cgatcagtgc cagggagaac
     3241 ccgtagctga ccggggtctt cccgtagacc atggtctcga agaccaggga ggccagcagc
     3301 gtggtccgca cgttgatgta gtt
//
LOCUS       DASRZS010000123         5310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3527447, whole genome shotgun sequence.
ACCESSION   DASRZS010000123 DASRZS010000000
VERSION     DASRZS010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3527447"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1745)
                     /locus_tag="VFH83_02955"
     CDS             complement(<1..1745)
                     /locus_tag="VFH83_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET6485350.1"
                     /translation="MVLFCPYSRRSEELLAKIGIGIDTGGTYTDAVAYDFGSHTLLSS
                     AKAQTTREDLSIGIGNALDALPREQVRAAEMVSLSTTLATNASVEDKGGRARLLFIGA
                     DRWVVERYGPEYGLPQDGEIFYLEANTTTAGEVLVEPDWEAFRRRADPWIQGACGIGI
                     VDRDAMDNGGVLERKGQELVSGAWDLPVVCGHEMFSDLNSLRRGSSVLLNARLVPLIT
                     EFLRAARLALTKRSVTAPVLILRSDGTLMSERFAVHRPVETLVCGPAASVMGGLTLSA
                     EKDSVIIDMGGTTTDVAIIEGGVPRRAREGVDAGKWKTLVKGLSVRSFGLGGDSAVRW
                     DRNGRMRLEPTRLLPLSVAAARWPSVTTKLRGLLAARRSHTLPLHELFCPVRDIAANP
                     AYTAMEREFCRSIQAGPLLFEEAAEAIGTSPYNLDVSRLEEEGVVLRSGLTPTDIMHL
                     RGDFTRFDAEASALGVTFVSACVGLSRDGLLEAVYDAVKRALYGTVVRVLLEDQEPSL
                     TGLDELIDRSWDLAKRRPGAGLLGFRFATPAALVGIGAPIHIFLPAVAEALGTRCVIP
                     ANAGVANALGAIAGT"
     gene            1727..1930
                     /locus_tag="VFH83_02960"
     CDS             1727..1930
                     /locus_tag="VFH83_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485351.1"
                     /translation="MGRRVPRYGGLDFPPDKVLAATVQTRVVNTYATTHVRNKTELGH
                     LLRVDGADPSEGHDSEGLGGALR"
     gene            complement(1939..2856)
                     /locus_tag="VFH83_02965"
     CDS             complement(1939..2856)
                     /locus_tag="VFH83_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6485352.1"
                     /translation="MEPRVRIVDLEDVPAVARALDSPIRRRMLEMLFEHPLTVSRLAK
                     ALSIPQSTCTVNLQVLKEAKLVRASPESTRLLVTSFEEVVIPLRGSQMAADTSAMEID
                     MPIGLYSAYEVHAPCGLLSEDRIIGYLDIAESFLDTHRASAQMIWLSNGYLEYPFPMS
                     PNMVPAGKRIKALAVVAEVCSEYPDYKLDWPSDITVWISGVEVGTWTCPGDMGGTRGR
                     LTPEWWPTHSTQFGFRKSWRIAEDGCFIDGVKVSDVTVSAVPLDRNLTVRIGIKDHAE
                     HKGGFNLFGRKFGNYEHDLILRFEFGPKG"
     gene            3014..4816
                     /locus_tag="VFH83_02970"
     CDS             3014..4816
                     /locus_tag="VFH83_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET6485353.1"
                     /translation="MPCSKFANHGNPWRSNLTVIPRPEHPRPDFERTEWINLNGVWAF
                     EPDSYEVFSGKDRLLHAPLKETITVPFPYQSRLSGIGRTGHHPCVWYRRSFEVPAPWL
                     SQRVQLHFGAVDYEARVWVNGHFVGEHRGGHVPFEFEIQRWLVTGPNELTVRAADAFD
                     ISQPRGKQAWAEPFGCWYTPTTGIWQTVWLESVPEVYTRSIRVDADPSSGEVTVIAVP
                     NQPAAGLTVRSVALRDGAQIAASQAPMAYPSTRLTFRVPNIAPWSPESPALYDLVVTL
                     EEGDRVLDRVRSYFGARTVTLERSSVLLNGKPCYQRLVLDQGFWPDGLYTAPTDEALK
                     ADVELALALGFNGCRKHQKVEDPRFLYWADRLGFLVWGEFPAPYEFSLAAQARFLPEW
                     QTAIQRDINHPSIVAWVPYNESWGIPGVGKDPTVQDWVRTVVGLTRFLDPTRLVVDNE
                     GWEHVDSDLIGYHTYAPTGEQLTRELEVALATARGQGAEGDGGPGLRRLMADGVPLPP
                     RPLMLTEFGGIGFSSGAADGASWGYHGVPGDLDAFRQRFESIFVGLRRIPDICGWVYT
                     QLTDVQQEINGLLTPDRRNKFDPLWVRRWVSGGG"
     gene            4934..>5310
                     /locus_tag="VFH83_02975"
     CDS             4934..>5310
                     /locus_tag="VFH83_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019909528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6485354.1"
                     /translation="MVALLALLCAVGAFAAGGSEKGSSGGPVTLKFTYWGSPQEKAAV
                     DASIQAFTAKYPNIKVNAIHIPTDYDTKITTMVAANEAPDMGYLESATIAFPLAEQGK
                     LVNLQELFDKEPDFAKTILPNLG"
BASE COUNT          926 a         1752 c         1715 g          917 t
ORIGIN      
        1 atggtgcccg cgatggcgcc taaagcgttg gccacccccg cgttggcggg aatcacgcac
       61 cgcgtcccca gcgcctcggc cacggccggt aggaagatgt ggatgggcgc tccgatgcct
      121 acgagcgccg caggagtcgc gaatcggaat cccagaagac ccgccccggg tcggcgcttg
      181 gcaaggtccc agctccggtc gatcagctcg tcgagccccg tcagggaggg ctcctggtct
      241 tccagcagca cccgcacgac ggttccgtac agcgcgcgct tcaccgcgtc gtacaccgcc
      301 tccaggagcc cgtcccggct cagacccacg caggcggaga cgaacgtcac gccgagcgcg
      361 gaggcctcgg cgtcgaaccg ggtaaagtct ccccggaggt gcatgatgtc cgtcggggtc
      421 aggccgcttc gcagcacgac cccttcctcc tcgagccggc tcacgtcgag gttgtagggg
      481 ctcgtgccga tggcctcggc ggcctcttcg aagaggagcg ggccggcctg gatgctcctg
      541 cagaactccc gctccatggc cgtgtacgcg ggattggcgg cgatgtccct gacggggcag
      601 aagagctcgt ggaggggaag ggtgtggctc cttcgggcag ccaggagacc ccgcagcttc
      661 gtcgtgaccg acggccaccg tgccgcggca accgagagcg gcaggagccg cgtgggctcc
      721 agcctcatcc tgccgttgcg gtcccaacgg accgcgctgt ccccgcccaa cccgaaggag
      781 cggacgctca gacccttcac cagggtcttc cacttgccgg cgtccactcc ctcgcgggcc
      841 cgtcggggca ctccgccttc gatgatggcg acgtcggtgg tcgtcccacc catgtcgatg
      901 atcacgctgt ccttctcggc ggacagggtg aggccgccca tgacgctggc ggccggaccg
      961 cagaccagcg tttccacggg ccggtgcacg gcaaaccgct ccgacatgag cgtgccgtcg
     1021 cttcgcagga tgagcaccgg cgcggtgacc gacctcttgg tcagggccag ccgggccgcg
     1081 cgcaggaact cggtgatgag gggaaccagg cgcgcgttca gaaggacgct ggatccccgt
     1141 cgaagggagt tcaggtcgga gaacatctcg tgcccgcaga ctaccggcag gtcccaggcg
     1201 ccgctcacca gctcctgtcc cttgcgctcc agcaccccgc cgttgtccat cgcgtcacgg
     1261 tcgacgatcc cgatcccgca ggcgccctgg atccacgggt cggcccgccg ccgaaaggct
     1321 tcccaatcgg gctccaccag gacctctccg gcagtcgtgg tgttcgcctc caggtagaag
     1381 atctctccgt cctgcggcag accgtattcg gggccatagc gttccacgac ccaccggtcc
     1441 gccccgatga agagcagacg cgcgcgtccc cccttgtcct ccacgctggc gttcgtggcc
     1501 agcgtcgtgg agagagacac catctcggcc gcccgcacct gttcccgcgg cagcgcatcc
     1561 aacgcgttgc cgatcccgat cgaaaggtcc tctcgggtcg tctgggcctt ggcggaggag
     1621 agcagggtgt gggaaccgaa atcgtatgcc acggcatccg tgtacgtccc acccgtatcg
     1681 atgccgattc cgatctttgc caggagctcc tccgatcgtc gcgagtatgg gcagaagagt
     1741 accacgatac ggcggtcttg acttcccgcc ggacaaggtc cttgcggcca ccgtccagac
     1801 gcgcgtcgtc aatacgtacg caacgaccca cgtcaggaac aagaccgagc tggggcatct
     1861 cctgcgggtc gatggagcgg atccatcgga aggtcacgac tccgaggggc tcggtggggc
     1921 gcttcgctga acgtactcct accccttggg gccgaactcg aagcgcagga tcaggtcgtg
     1981 ctcgtagttg ccgaacttgc ggccgaagag gttgaacccg cccttgtgct ccgcgtgatc
     2041 cttgatcccg atgcggacgg tcaggttgcg atccagcgga accgcggaga cggtgacatc
     2101 ggacaccttc acgccgtcga tgaaacagcc gtcctcggcg atccgccacg acttcctgaa
     2161 cccgaactgg gtggagtggg tgggccacca ttccggggtc aggcggccac gggttccccc
     2221 catgtcgcct ggacaggtcc aggtgcccac ctccactccg ctgatccaga ccgtgatgtc
     2281 cgatggccag tccagcttgt agtcggggta ttccgagcag acctcagcca cgacggccag
     2341 ggccttgatc cgtttgccgg caggcaccat gttgggcgac atcgggaacg ggtactcgag
     2401 gtagccgttc gacagccaga tcatctgcgc cgaggcccgg tgggtatcca gaaaggattc
     2461 ggcgatgtcg agatacccga tgatccgatc ctccgacagc agaccgcagg gggcgtgcac
     2521 ttcgtacgcg gagtacaatc cgatgggcat gtcgatctcc atcgcgctcg tgtccgcggc
     2581 catctgagag cccctaagcg ggatgactac ctcctcgaaa ctggttacca ggagccgagt
     2641 gctctccggt gaagcgcgta ccagcttcgc ttccttgagg acctggaggt tcacggtgca
     2701 ggtggactgc ggaatgctca gggctttggc gaggcgagag acggtgagcg gatgctcgaa
     2761 gagcatctcc aacatccgcc ttcggatggg ggagtccagg gctcgggcaa cggctgggac
     2821 gtcctcgaga tccacgatcc ttacccgcgg ttccatgaga gtgattatca actggacatg
     2881 atgacttcgt caatatggca ggactaccca tcacattctg atgaacaagg ccagcaaacg
     2941 acttatcata gcctttccgt gataattggg tcgttttcgc ttgacttcag aaccatccat
     3001 caactatact tgaatgccat gtagcaagtt cgccaaccac ggaaatccat ggaggtcgaa
     3061 tctgactgtc atcccgagac cagagcaccc cagacctgat ttcgagcgta cggagtggat
     3121 caacctcaat ggcgtctggg cgttcgaacc cgacagctac gaggtgttct ccgggaagga
     3181 ccggctgctc cacgcgcctc tcaaagaaac catcacggtt cccttcccct accagagccg
     3241 actctcaggg atcggtcgca cggggcacca tccctgcgtg tggtaccgac gctccttcga
     3301 agtgccggct ccctggctca gccagcgagt ccagctccac ttcggcgccg tggactacga
     3361 ggcgcgcgtt tgggtcaacg gtcatttcgt cggcgagcat cgtggcgggc acgtgccctt
     3421 cgagttcgag atccagcgct ggctggtgac gggaccgaac gagctgacgg tccgtgcggc
     3481 ggacgccttc gacatcagtc agccgcgggg aaagcaggca tgggccgagc ctttcggatg
     3541 ttggtacacg ccgacgacgg gcatctggca gacggtatgg ctggagagcg tgccggaggt
     3601 ttacacgcgg tccatccggg tcgatgcgga cccgagcagc ggtgaagtga cggtcatcgc
     3661 cgtgcccaac cagccggcgg ccgggctcac ggtccgttcc gttgcccttc gggatggcgc
     3721 gcagatcgcg gcgagccagg cgccgatggc ctatccttcc acgcgcctca ccttccgtgt
     3781 cccgaacatc gctccctgga gccccgagtc ccccgccctc tacgacctgg tggtcacgct
     3841 ggaggaggga gaccgggtcc tggaccgcgt gcgcagctac ttcggggcgc gcacggtaac
     3901 cctggagcgg tcctcggttc tcctgaacgg caagccctgc tatcagaggc tcgtgctgga
     3961 ccagggattc tggccggatg ggttgtatac cgctcctacg gatgaggcgc tgaaggcgga
     4021 cgtggagctg gcgcttgccc tcgggttcaa cggatgccgc aagcaccaga aggtggagga
     4081 tccgcgcttt ctctattggg cggatcggct cgggtttctc gtctggggag agtttccggc
     4141 gccttacgag ttctccctcg cggcgcaggc ccggttcctt cccgaatggc agaccgcgat
     4201 ccagagagac atcaatcacc ccagcatcgt ggcctgggtt ccctacaacg aatcctgggg
     4261 catccccgga gtaggaaagg atcctaccgt acaggactgg gtccgcacgg tagtcggcct
     4321 cacccggttc ctggacccga cccggctcgt ggtggacaac gaggggtggg agcatgtcga
     4381 ctcggacctc atcggctatc acacctacgc gcccacggga gagcagttga cgcgggagct
     4441 cgaggtggcg ctggccacgg ctcgcggcca gggggcggag ggcgatgggg ggcccggcct
     4501 caggaggctg atggcggacg gcgtcccgct tccccccagg ccgctcatgc tgacggagtt
     4561 cgggggcatc gggttctcca gcggcgccgc cgatggcgcg agctggggct accatggcgt
     4621 accaggggac ctcgacgcgt tccgtcagag gttcgagagc atcttcgtgg ggctccgccg
     4681 gatccccgac atctgcgggt gggtgtacac gcagctcacc gatgtccagc aggagatcaa
     4741 cggtctgctc accccggatc gccggaacaa gttcgatccc ctttgggtcc gccgctgggt
     4801 ctccggcggc ggatgaccgg aaaggaggca ccagcccggg gcttacgttc cttggattgg
     4861 aggcgggcgc agacgtcccg ctcgacacga acaggaggat ggaatgcatc gctcaggtct
     4921 atttcggatt tcgttggtag cgctgctcgc gctgctctgc gccgtgggcg cctttgccgc
     4981 gggaggctcc gagaagggct cctccggcgg cccggtcacg ctcaagttca cgtactgggg
     5041 cagcccgcag gagaaggcgg ccgttgacgc gtcgatccag gccttcacgg ccaagtaccc
     5101 gaacatcaag gtcaacgcca tccacatccc gacggactac gacaccaaga tcaccacgat
     5161 ggtagcggcc aacgaggctc cggacatggg atacctcgaa tccgccacca tcgccttccc
     5221 gctggccgaa cagggcaagc tggtcaacct ccaggagctc ttcgacaagg agcccgattt
     5281 cgcgaagacc atcctgccca acctcggcta
//
LOCUS       DASRZS010000124         5574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1991365, whole genome shotgun sequence.
ACCESSION   DASRZS010000124 DASRZS010000000
VERSION     DASRZS010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991365"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFH83_02980"
     CDS             complement(<1..713)
                     /locus_tag="VFH83_02980"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HET6485355.1"
                     /translation="MRAVIRAVDFAVPCRRVANEEFSATLDTSDEWIRSHTGIRFRHI
                     ADPSMATSDLAFDACKKALEKANVSPQELDLLLVATVTGDYIGFPSVSCIVQGKLGAS
                     HAGAMDLVAGCTGFIYGLETARGFVLSGFAKNVLVAGADTLSRIINWQDRNTCVLFGD
                     GAGAALVTGEEGDGDRGIIRSFLRADGTGANLLERSVGGTRFPFGNGSDPLGTYLKMD
                     GRQVYNFAVKVVSEGIVEML"
     gene            879..1820
                     /locus_tag="VFH83_02985"
     CDS             879..1820
                     /locus_tag="VFH83_02985"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET6485356.1"
                     /translation="MKTCFLFPGQGAQYPGMARDLFEASPEVRELFSVASGTSGMDAR
                     KLLFESTAEELKATDRTQIAVTLASLCSGWVLRSSGARMDGCAGFSLGEYAALCMAGV
                     ISLKDVFPIVKLRGELMEKAARGLDSSSGAAGMVAVLGIPAERVASVVEPLAKEGAHL
                     ANYNSPGQMVISGTADGLQRAEKALKDAGAKRLIRLAVSGPFHSPLMVEARKAFDEAL
                     KEYPFDDPQVPVYSNVTGRPISTGAEARELCGRQLVSLVRWVTVEERLLADGFDRFLE
                     AGPGTVLAGLLRALRPDLTCAPAGTLEGIQKATGVAA"
     gene            1817..2560
                     /gene="fabG"
                     /locus_tag="VFH83_02990"
     CDS             1817..2560
                     /gene="fabG"
                     /locus_tag="VFH83_02990"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET6485357.1"
                     /translation="MTLKGKRAIVTGGSRGIGNGIVTTFLSYGATVYALSRSQPSNAD
                     ALAEAAKAGGGAFQWRQADVTREEEMSTLLEAIVAEAGGVDVLVNNAGITRDGLIFRM
                     STADWDEVLRANLTAAFIACRSVSRVMIKQRAGSIINISSVSGIMGNAGQVNYSASKA
                     GLIGLSKSLAREVASRGVRVNAIAPGFIETDMTKAIGEKARDALISQIPLARVGAAGD
                     VAALAAFLASDASAYITGQVIAVDGGLSM"
     gene            2575..3816
                     /gene="fabF"
                     /locus_tag="VFH83_02995"
     CDS             2575..3816
                     /gene="fabF"
                     /locus_tag="VFH83_02995"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET6485358.1"
                     /translation="MQRRVVVTGMGTVNPLGNNVKDFWRAIMAAENGIDKLTRFDTAN
                     YSAQIAGQVKDFAPEKVLESRDARRMDRFTQYAMVASIEAMGDAGLSPGQANAERFGV
                     ILGNGIGGIETLEAQFRRVVDKGPKGVHPLFVPMMISNEAPGNVAIRFQAFGPCSCVV
                     TACASSNDAIGHAWQLIRDNIADVMIAGGTEAPLTPLGLAGFCQLQAVSTRNDDPAHA
                     SRPFDKDRDGFVMAEGSGIVVLEELEHAKARGARIYAELAGYAATCDANHLTAPHPEG
                     RGAIKAIGLAIESAGMSPCEVDYVNAHGTSTPINDPIETKAIRAVFGEHAPKLKVSST
                     KSMTGHLLGAAGGIEAIVTVKAIEDQFFPPTRNLDQPDPECDLDYVPHTGARGRIRAA
                     ISNALGFGGHNSVVLFKEYKA"
     gene            complement(3851..4744)
                     /locus_tag="VFH83_03000"
     CDS             complement(3851..4744)
                     /locus_tag="VFH83_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HET6485359.1"
                     /translation="MNTFSEIIDAARRAGPARFVVAQAADATILEALEEARRAGFARP
                     VLVGLPDEIEKEARVQGISLSGWEMVPVAAKTDSAAEAVKTIAHGDGDILVKGLLQTA
                     DLLRAVLNKANGLNTGRTLSHVGVFRFEGSERFYLVTDAAISIAPTYEQKIDIVQNAV
                     DLAHRLGIETPVVAILCAIETVNPDMQATVDAACLAKMAERGAIRGARVEGPLALDNA
                     VSVEAARHKGITSDLAGRADILVAPNIEAANILYKALIFFAKAEEAGIVMGARAPIVL
                     NSRSDSVRNKLYSLALGALAR"
     gene            complement(4945..>5574)
                     /locus_tag="VFH83_03005"
     CDS             complement(4945..>5574)
                     /locus_tag="VFH83_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485360.1"
                     /translation="IPDISVSRAAFREITSADQVDVYEFQAAKGQELYIQMTVPLLDR
                     EKGFAPAFVLVYTGGQPTEFADPAIDKGMVVDPPHAVVDNVLPHNDEQEPSLIGISYD
                     SSADQVFDEPFTGTRYWVRQTLTVKAPMDGAYRIGVYVPNGATGKYVLAPGKREQFGF
                     GDLLTFPGVRMTVRQFCEQPIWPDVLVLGLLGAAALSGIGLGIYALAVL"
BASE COUNT         1001 a         1779 c         1852 g          942 t
ORIGIN      
        1 agcatttcca cgatgccttc ggaaaccacc ttcacggcga agttgtagac ctgtctgccg
       61 tccatcttga gataggtgcc gagagggtcc gagccgttcc caaacgggaa ccgggtcccg
      121 cccaccgagc gctccaggag gttggccccc gtgccgtccg cgcgcaggaa ggagcggatg
      181 atcccgcggt ccccgtctcc ctcttcgccg gtcaccagcg ccgcgccggc gccatcgcca
      241 aagagcacac aggtattccg gtcctgccag ttgatgattc ggctgagcgt gtcggcgccg
      301 gctaccagga cgttcttcgc gaagcccgag agcacgaagc ctcgagccgt ttccagcccg
      361 tagatgaagc ccgtgcaacc cgccaccagg tccatggccc ccgcgtggct ggcgcccagc
      421 tttccctgga cgatgcagga aacggacggg aaaccgatgt agtctcccgt gacggtagcg
      481 accaggagga ggtcgagctc ctggggcgag acgttggcct tttccagcgc cttcttgcat
      541 gcatcgaagg ccaggtccga ggttgccatg gatgggtcgg caatgtggcg gaaccgaatg
      601 ccggtatggg aacgaatcca ctcgtcggag gtatccaggg ttgcggagaa ctcttcgttg
      661 gccactctgc gacacggcac cgcgaagtcc acggccctga ttactgcacg catgccgcca
      721 tagtaatgcg attgaaatga tcggtcaacc gagttgccag aaagtcaaat tcctcttata
      781 ttgatcagca attcagatgt ctaagcagga caaacactta taccagatcg aaccatcgcc
      841 ttgaccagaa aatcgagtcc atggtatttt ggcaggatat gaaaacctgt ttcctgttcc
      901 ccgggcaagg ggcgcagtat cccggcatgg caagggattt attcgaagcc agcccggaag
      961 tgcgagagct gttctcggtg gcatccggca catcgggcat ggacgcgcgg aagctgttgt
     1021 tcgaatcgac tgccgaggag ctcaaggcca cggatcgtac ccagatagcc gttacccttg
     1081 ccagcctatg ctcgggttgg gtgcttcgga gcagcggggc acggatggac gggtgcgccg
     1141 gcttcagcct cggcgaatat gcggctctgt gcatggccgg ggtcatctct ctcaaggacg
     1201 tcttcccgat cgtgaagctc cgaggcgagc tgatggagaa ggcggcccgg gggctggatt
     1261 cctcgtccgg cgccgcgggg atggtcgcgg tgctcggaat cccggcggag cgcgtcgctt
     1321 ccgtggtgga gcctctggcc aaagaagggg cgcacctggc caactacaac agcccgggtc
     1381 agatggtcat ctcgggtact gcggatgggc ttcagcgcgc ggagaaggcc ctcaaggatg
     1441 ctggggccaa gcgcctgatc cgacttgccg tctcggggcc cttccattcg cccctgatgg
     1501 tggaggcgcg caaggcattc gacgaggcgc tcaaagagta cccgttcgac gatccgcagg
     1561 taccggtgta ttcgaacgtg acgggccgcc ctatctccac cggggccgaa gcgcgcgagc
     1621 tgtgcggcag gcaactggtc tccctggtgc gctgggtgac ggtggaggag cggctcctgg
     1681 cggatgggtt cgatcgattc ctggaggccg ggccgggcac ggtgctggcg ggcctgctgc
     1741 gcgcccttcg gccagacctt acctgcgcgc ccgcgggcac gctggagggc atccagaagg
     1801 ccacgggggt cgcggcatga cgctgaaagg caaacgcgcc atcgtgaccg gcggatcccg
     1861 ggggatcggg aacggcatcg tgaccacgtt tctttcctac ggcgccacgg tgtacgccct
     1921 ttcccgttcg cagccgtcca acgcggacgc gctggcggaa gcggccaagg caggcggagg
     1981 cgccttccag tggcgccagg cggatgtgac gcgggaggag gagatgtcca cgctcctgga
     2041 ggccatcgtg gccgaggcgg gtggcgtgga cgtgctcgtg aacaacgctg gaatcacccg
     2101 ggacgggctg atcttccgga tgtctaccgc ggattgggat gaggtgctcc gggccaacct
     2161 gacggcggcg ttcatcgcgt gccgctccgt gtcccgggtg atgatcaagc agcgggcggg
     2221 ctcgatcatc aacatctcct ccgtcagtgg tatcatgggc aacgctggac aggtgaacta
     2281 ctctgcctcc aaggccgggc tgatcgggct gtccaagagc ctggcgcggg aagttgcctc
     2341 ccgcggggta agggtgaacg ccattgcccc cggtttcatc gagacggaca tgaccaaggc
     2401 aattggggag aaagcgcggg acgcgctgat ctcccagatc cctctggccc gggtgggggc
     2461 cgcgggggac gttgcggcgc tggccgcgtt cctggcctcg gacgcatccg cgtacatcac
     2521 ggggcaggtc atcgccgtcg acggcggact gtcgatgtag ggcaaggaga aggtatgcag
     2581 cgtagagtcg ttgtgaccgg catggggacc gtcaatccct tgggcaacaa cgtgaaggac
     2641 ttctggcggg cgatcatggc cgcggagaac gggatcgaca agctcacccg gttcgacacc
     2701 gccaactaca gcgcccagat cgcggggcag gtgaaggact tcgcgccgga gaaggtgctg
     2761 gagtcgcggg acgcgcggcg catggaccgc ttcacccaat acgccatggt ggcctccatc
     2821 gaggccatgg gtgacgcggg gctgtctccc gggcaggcca acgcggagcg cttcggcgtc
     2881 atcctgggga acgggatcgg cggcatcgag acgctggagg cccaattccg aagggtcgtg
     2941 gacaagggcc ccaagggcgt gcatccgctg ttcgtcccca tgatgatctc caacgaggcg
     3001 ccgggcaacg ttgccatccg cttccaggca tttggcccgt gctcctgcgt ggtgaccgcg
     3061 tgcgcctcct ccaacgacgc catcggacac gcctggcagc tcatccggga caacatcgcc
     3121 gacgtgatga tcgccggggg gaccgaggca ccgctcacgc cgctcgggct ggccgggttc
     3181 tgtcagctcc aggcggtgag cacgagaaac gacgatccgg ctcacgccag ccgtccgttc
     3241 gacaaggacc gagacggctt cgtgatggcg gaaggctccg gtatcgtggt gctggaggag
     3301 ctggagcacg caaaggcccg cggggcccgg atctacgccg agctggcggg gtacgcagcc
     3361 acctgtgacg ccaaccacct gaccgctccg caccccgagg gccgcggcgc catcaaggcc
     3421 atcggcctgg ccatcgaatc cgccgggatg tccccgtgcg aggtcgatta tgtcaacgcc
     3481 cacggcacct cgacgcccat caacgaccct atcgagacca aggccatccg ggcggtcttc
     3541 ggcgagcatg cccccaagct caaggtctct tccacgaaat ccatgaccgg acatctcctg
     3601 ggcgcggccg gcggcatcga ggccatcgtc accgtgaaag ccatcgagga ccagttcttc
     3661 ccgcctacgc gcaacctgga ccagccggac cccgagtgcg acctggacta cgtgccgcac
     3721 accggcgcgc ggggacggat acgagcggcc atctccaacg cgctggggtt cggcggacac
     3781 aactcggtgg tgctgttcaa ggagtacaaa gcgtaagggt cgggcgggtg acggggagaa
     3841 gcgcgctccg ctatcgcgcc agcgccccca gcgccagcga atacagcttg tttctcaccg
     3901 agtcgcttcg agagttgagg acgatgggcg cccgagcgcc catcacgatc ccggcctcct
     3961 cggccttcgc gaaaaagatc agcgccttgt agaggatatt ggcggcctcg atgttggggg
     4021 ccaccaggat gtccgcgcgg ccggcaaggt cgctcgtgat ccccttgtgc cgggccgcct
     4081 cgacgctgac cgcgttgtcc agcgccagcg gaccctctac gcgagcgccc cggatggcgc
     4141 cccgctccgc catcttggcc aggcacgccg catccaccgt ggcctgcata tcaggattga
     4201 ccgtctcgat ggcgcacagg atggcgacga ccggcgtctc gatccccagg cgatgcgcaa
     4261 ggtccacggc gttctgcacg atgtcgatct tctgctcgta ggtaggggcg atggagatgg
     4321 ccgcgtcggt cacgaggtag aatcgctcgg acccctcgaa gcggaagacg ccgacgtggg
     4381 agagggtgcg tcccgtgttc agcccgttcg ccttgttgag gaccgcgcgc aggaggtcgg
     4441 cggtctgcag caggcccttg accaggatat cgccgtcccc gtgggcaatc gtcttgaccg
     4501 cttccgccgc ggaatccgtt ttcgcggcga cgggaaccat ctcccaaccg ctcaaggaga
     4561 tcccttgaac ccgcgcttcc ttctcgatct catccggaag gccgaccagc accggccgcg
     4621 cgaacccggc tctgcgggcc tcttccaggg cttccaggat cgtggcgtcc gccgcctgcg
     4681 ccactacgaa ccgtgcgggg ccggcacggc gagccgcgtc gatgatctcg ctgaaggtgt
     4741 tcatgggtcc tcccgcgcca aaaaaaagcc ggacttcgcc ggctttacag aaatccgctc
     4801 gagggtctgc aggtcgcagg gtcgaacacg gattcaatga tggtaatgag ggcggtgccc
     4861 ccaatccacc tctagcgtat accctcggta ccccgttgtc aatgtcgggg agaaaagccg
     4921 ccggcaggga gatcgcgcgt gaatctacag cacggccagc gcatagattc cgagcccgat
     4981 cccgctcagc gcggcggcgc ccagcaggcc caggacaagc acatccggcc agatgggttg
     5041 ctcgcagaac tggcgaaccg tcatccgcac gccagggaag gtgagcaggt ctccgaaacc
     5101 gaactgctcc cgcttcccgg gggcgagcac gtacttcccc gtggcgccat tcgggacgta
     5161 gacgccgatc cggtaggcgc cgtccatcgg cgccttcacc gtgagcgtct gtcggaccca
     5221 gtaccgggtt cctgtgaagg gctcgtcgaa aacctggtcc gcgctcgaat cataagagat
     5281 cccgatgagg gacggctcct gctcatcgtt gtgcggcaac acgttgtcca ccacggcatg
     5341 gggagggtcc acgaccattc ccttgtcgat cgccgggtcg gcaaactcgg tgggctggcc
     5401 tccggtgtac acaaggacga acgcgggcgc aaaccccttc tcccgatcca gcaatgggac
     5461 tgtcatctgg atatagagct cctggccttt cgcggcttgg aactcgtaga catccacctg
     5521 gtctgccgag gttatctccc ggaaggcggc gcgggagacg ctgatgtccg gaat
//
LOCUS       DASRZS010000125         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5454693, whole genome shotgun sequence.
ACCESSION   DASRZS010000125 DASRZS010000000
VERSION     DASRZS010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5454693"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VFH83_03010"
     CDS             <1..743
                     /locus_tag="VFH83_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485361.1"
                     /translation="GQTRKTLEDVSLLAGPGEFIGITGTIGSGKSTLINLITGLLRPP
                     RGTVFVNGIDVRDIEPAALFARIGIVSQSPFLFSRTLAENIAMGKDGTGDEADVQKAA
                     ELAGLAGDIAAFTQGYQQMIGERGITLSGGQKQRTAIARALMKDCPVVILDDSLSSVD
                     AGTEATILSNLRSYRGARTLIVVSHRISPLKGADRIYVLDQGRIVEQGSHGELVEQHG
                     LYERLVRFQQMEKTLEMPTSREPGWSKN"
     gene            743..>2290
                     /locus_tag="VFH83_03015"
     CDS             743..>2290
                     /locus_tag="VFH83_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485362.1"
                     /translation="MAEKQRPLETRLLRRLLPFLAPYAWLGIGALLCIVIVDAAGVLQ
                     PWLVKRAVDTNIAARDLVGLGRTALLLGLALLAGYGFQVVSGYGIQYLGQRLLVDLRM
                     NLFRKVLRLSNDYFDRTPVGQTLTNLSNDLEALREFIADGIVSVAGDLLKVAFIVVAM
                     LLINVELALVAFITIPLFVTATLLFRAGIRAGFRQVRKANGEINVALSETISGVREIH
                     QFGYEAESRASFDRNNRSYLAAYLKVVNAYALFFPAIEIVTYTSMILTLVFTHFWLGT
                     RVQVGEIFAFFAYITMFFMPLRQLAEKFNLLQSAVAATERIFRLMDEPVSVVSPTADL
                     PHPIASPAELVFDEVRFHYSPESPILRGVTFRVAPGEKVAIVGPTGAGKTTLISLVTR
                     LYDVQAGRILIDGIDVKEIPLDRLRRVVATVPQDVFLFTGTVAENISLYDPAVDWRMV
                     EHAAQEVRANRFVAGFPKGYGEAVGEEGRRLSEGQRQLLGLSRAFARDPAIVILDEAT
                     SNIDSETE"
BASE COUNT          376 a          759 c          759 g          396 t
ORIGIN      
        1 cgggccagac ccgcaagacg ctggaggacg tctccctgct cgccgggccg ggggagttca
       61 tcgggatcac cgggacgatt ggcagcggaa agtctaccct gatcaacctc atcacggggc
      121 tcctgcggcc cccccggggc acggtgttcg tgaacgggat cgacgtgcgt gacatcgagc
      181 ctgccgcgct cttcgccagg atcggcatcg tttcgcaatc gcccttcctc ttttcccgta
      241 cgctcgccga gaacatcgcc atggggaagg acgggacggg cgacgaggcg gacgtccaga
      301 aggcggccga gctggccggc ctggcgggcg acatcgcggc tttcacccag ggctaccagc
      361 agatgatcgg ggagcgtggg atcaccctct ccggcggcca gaagcagcgg accgccatcg
      421 cgcgggcgct catgaaggac tgcccggtcg tcattctgga cgactccctg tccagcgtgg
      481 acgccggcac cgaggcgacg atcctctcga acctgcggtc ctaccggggc gcgcggacgc
      541 tgatcgtggt gtctcatcgc atctctccgc tcaagggagc ggaccgcatc tacgtgctgg
      601 accagggcag gatcgtggag caagggtcgc acggagagct ggtggagcag cacggcctct
      661 acgagaggct ggttcggttc cagcagatgg agaagaccct ggagatgccc acctcccgag
      721 agccgggctg gagcaagaac tgatggcgga aaaacagcgt cccctcgaaa cgcggctcct
      781 gcgaaggctg ctgcccttcc tggcccctta cgcctggctg gggatcggag cgctcctgtg
      841 catcgtgatt gtggacgcgg ccggtgtcct gcagccatgg ctggtgaagc gggccgtgga
      901 cacgaacatc gccgcgcggg acctggtggg cctgggacgg accgccctcc tcctggggct
      961 cgcgctcctg gccggctacg ggttccaggt ggtctcggga tacgggatcc agtacctggg
     1021 gcagaggctc ctggtcgatc tgaggatgaa cctcttccgc aaggtccttc gtctctccaa
     1081 cgactacttc gaccgcacgc ccgtcgggca gaccctcacc aacctgtcca acgacctgga
     1141 ggcgctccga gagttcatcg cggacgggat cgtgagcgtg gccggggacc tcttgaaggt
     1201 ggccttcatc gtcgtggcca tgctcctcat caacgtggag ctggccctcg tggcgttcat
     1261 caccattccc ctgttcgtga ccgccaccct gctctttcgc gcagggatcc gcgccgggtt
     1321 ccgccaggtc cgcaaggcga acggggagat caacgtggcg cttagtgaga ccatctcggg
     1381 agtccgggag atccaccagt tcggctacga ggcggagagc cgcgcctctt tcgaccgcaa
     1441 caaccgaagc tacctggcgg cctacctcaa agtggtgaac gcgtacgcgc tgttcttccc
     1501 ggccatcgag atcgtcacgt atacgagcat gatcctcacg ctcgtcttca cccacttctg
     1561 gctcgggacc cgcgtacagg tgggtgagat cttcgccttc ttcgcgtaca tcacgatgtt
     1621 cttcatgccg ctgcgccagc tcgcggagaa gttcaacctg ctgcagtccg cggtggccgc
     1681 cacggagcgg atcttccgac tcatggacga gccggtgagc gtggtctccc cgaccgcgga
     1741 tctcccccac cccatcgcca gccccgccga gctggtcttc gacgaggtcc ggttccacta
     1801 cagcccggag tcgccgatcc ttcgcggcgt aacgtttcgg gtcgcgccgg gggagaaggt
     1861 ggccatcgtc ggccccacgg gcgccggcaa gacgacgctc atcagcctgg tgacccggct
     1921 gtacgacgtt caggccggcc gtattctgat cgacgggatc gacgtgaaag agatcccgct
     1981 ggaccggctc cgccgcgtgg tagccacggt tccgcaggac gtcttcctct tcacggggac
     2041 cgtggcggag aacatctccc tgtacgatcc cgcggtggac tggcgcatgg tggagcatgc
     2101 cgcccaggag gtgcgagcca accggttcgt ggccggcttc ccgaagggtt atggagaagc
     2161 cgtgggggag gaaggccgcc ggctctccga gggacagcga cagctcctgg ggctctcccg
     2221 cgcgtttgcc cgggacccgg ccatcgtgat cctggacgag gccacgtcga acatcgactc
     2281 ggagaccgag
//
LOCUS       DASRZS010000126         8065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1603566, whole genome shotgun sequence.
ACCESSION   DASRZS010000126 DASRZS010000000
VERSION     DASRZS010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603566"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="VFH83_03020"
     CDS             complement(<1..112)
                     /locus_tag="VFH83_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6485363.1"
                     /translation="MDRIYLDNNATTIVDPRVKEAMEPFWSQLYGNPNSLH"
     gene            complement(178..1185)
                     /gene="lgt"
                     /locus_tag="VFH83_03025"
     CDS             complement(178..1185)
                     /gene="lgt"
                     /locus_tag="VFH83_03025"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET6485364.1"
                     /translation="MLAYIPYPSWIHPQIVPFLPVRWYGLMYLIAFAIAYLLFRYQLR
                     EQSIKPEKDVVLDMFFWGIVGLLVGARAFAVTIYDSTGYYLHHPLQIILPVAVVDGKI
                     RLTGIAGMSYHGGLVGATIAIVIFLRVKKISVLQWGDMLVSGIPLGYTFGRLGNFINA
                     ELYGRVTRVPWGMVFPGAESFSSGQPWVQDYARSVGMPLPTTGLVNLPRHPSQLYEAF
                     FEGFVLWLIMWFIVRKRKPYDGFGIACYVIGYAVIRFFIEYVRQPDKGIDWPIRFVHV
                     DNPVYQFTPFNFTTGQILNVLMITGAVVFLYVLRRRARLNAAAPASAPPSARKLRKKL
                     K"
     gene            complement(1204..4161)
                     /locus_tag="VFH83_03030"
     CDS             complement(1204..4161)
                     /locus_tag="VFH83_03030"
                     /inference="COORDINATES: protein motif:HMM:NF015894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein A"
                     /protein_id="HET6485365.1"
                     /translation="MSIRGDLRLEIDELGLEVRVTIQPDAGGAELSPEVLHTVLAGKK
                     VRFGIDEQAIEHAFRALNRSRPEPVSFVAAIGIPPEPTVAEKVTFESLPIPSRLSERA
                     RAVLGKAGQPKPYRLREVKVKREKKVIKKGFLASKETVETVVEKKTVKEPATVDLAVT
                     ALGYATPGRVVARVEPGKQGKEGKSVFGRSILAARPGEDAVLFCDGLERSGTEVKATV
                     TGFLRRGAAWAEVVPFNDHEVKVEASLDKLTCLLTVTPGDPGTPPPEAYEVLEAANRL
                     GFASRTLMPEPQIEALIHGALSGAAPVARKPISLSKDGSVEVVISEDQLSATLNIRKG
                     RGAGKTVTAADVGQAIRASRVAGFNAEELRKTLTTFFAGTDMELTGYPLVTGKPPKTG
                     KEAQIEWIVPFLSQAETRQRIVMLDVKRIPKIPSLEAFPLEKVEAMAEVAAGAPVLRV
                     SPGVGGTPGLDVFGIAIPVSKAGAPQVRIYEGLAMKRDEVVTTVPGILDKASEGMVIL
                     LRVRPHRDAELRVTISDDRMKATVTYIPPAGDGYPIPAEDVRYRIQKGGVVRGINETQ
                     LLQVMDHVNQGKALSDFEVAQGKMPAATPDERITFHAHLATGKASARRDGSVDFKAQD
                     RISSVEKGELIATLHPLIAEQADGWDVTGVVKPVSAEVRLGLQAGRGVRADRDSEGTT
                     RFYSERAGELTRDGAVLSVHESYTVPNDVDLTTGNVDFPGVIHVGGSIRSGFKVAAGQ
                     GLDVAKAVEAALVSAATSIVVGAGIKGEGRAVLRAGTDLTATFAEQATLVATGDVKLR
                     ESCVLCQVTCGGKLILETERGHLVGGRVRAYKGVLAQNIGSAVGVHTVVQFGEDFLLQ
                     EKFTRDQRIMDALQKKLTDLDTVLRQVVKAVEAAKSGTGGGVSVAVPKNLREMQEQRR
                     TLIKRVEELKVSLAAMHEKLRQPVPATVEVRGTLFPGSILECRGKRYEVTTPKNMITL
                     RFDPAQGKIVEAL"
     gene            complement(4267..4689)
                     /locus_tag="VFH83_03035"
     CDS             complement(4267..4689)
                     /locus_tag="VFH83_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485366.1"
                     /translation="MTARRALLLAGSFWELARFFLLTMLVALVLDASGVSGPGVYPWL
                     LLIGSPSLVVAAGALLLSLFPRRYEALIGMLRLGKLLGTFTFLLLVLSRALRAAQSIQ
                     LLVVGRVRVNGDRVAYSIFLVDALFLAFLMAWRPADSA"
     gene            4859..6271
                     /locus_tag="VFH83_03040"
     CDS             4859..6271
                     /locus_tag="VFH83_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6485367.1"
                     /translation="MKLLQTRERVVWTAVTAVLLAALAFFALSPRILAGTSDDETQAY
                     LGTLGDTFRYIRDNYVDQEKAQPKALYEGALKGMFESLGDPNSAYHTAKEWSRVTDTT
                     TGSFGGVGLVISTEKGLGARIDSAIEGTPAYRAGLTAGDLILKIDGKPMADVELNDVV
                     AQLRGVPHSTVTVTVRRGEAAQFDAVMTREVIEVPTVKYAVMPGGIGYLRITQFTAQT
                     AERTREAIRSFDDAGYTSLIVDLRQNPGGLLTAAVDVANLFIDQGLIVERKNNRVASE
                     NVRYSARRNRQMVAPTIPIAVLVDGGSASASEILSGALRDYHRASLFGTRTYGKGSVQ
                     TVQALGDGGFRLTTSRYYTPSGICVDKVGLQPDIEVKEPDLSEDQQKILGDLLNGSSL
                     KDFVKTNPQPTEAQVAEFVSGLHANGVTLSDRYLRRLVRNEVNRTNNNPPKYDLDFDI
                     VLQAAVKALEGHQIATGSAQ"
     gene            6323..7087
                     /locus_tag="VFH83_03045"
     CDS             6323..7087
                     /locus_tag="VFH83_03045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HET6485368.1"
                     /translation="MRQYLLPASYAGQPTLTVSGNDWRHLTRVLRLRVGDTLAARDVH
                     GTRYRMRIGSMGRTSCVVELAPQEAPADASPAPAQTGPRLTLLQCLPKGRKIDLIVRQ
                     ATEAGVSRIVPVLSERSLVRAGEERSARLRRIAEEAMEQSGRDRLPDIEDPRTLESIS
                     RDHWGTGILFYESAAGALPLHHLLAHGPAEVCILIGPEGGFAAAEVELVTEAGFRIAH
                     LGETVLRVETAALYAIGAVMTIVQERNDWKSVPRPE"
     gene            7084..7815
                     /locus_tag="VFH83_03050"
     CDS             7084..7815
                     /locus_tag="VFH83_03050"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HET6485369.1"
                     /translation="MKRIKVLDVPVDCVDPDVAVRVVESLLDNGLRNQIVFLSLRALM
                     RARHDPELLRSLRDAALVLPVSLAVVRGARFLKAGHPCLYNSFEYIIRILSVVEKAKG
                     SVYLLGSRKQILEVAEENLLGSFHGIRVVGRFYGYFPRTVEADIVTAIKKASPSLVLV
                     GTGVPGRDKWVLKHKRDLNPGIYLWAGYCFEIFAGKERQVSRKLHARGLSALSGIRRR
                     PWRVVGIFPYLYYQVLLVGYRLFKL"
     gene            complement(7828..>8065)
                     /locus_tag="VFH83_03055"
     CDS             complement(7828..>8065)
                     /locus_tag="VFH83_03055"
                     /inference="COORDINATES: protein motif:HMM:NF016102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HET6485370.1"
                     /translation="LDRLKARIRESAYPGKEFVDLVGILIGSLLLLTPGFVTDVVGYL
                     LLLPFLRDRLAKVIARKLDSHFREVYEYLKLSGV"
BASE COUNT         1397 a         2698 c         2652 g         1318 t
ORIGIN      
        1 cgtgcaggga gttggggttg ccgtagagct gggaccagaa cggctccatg gcttccttca
       61 cccgagggtc cacgatcgtc gtggcgttgt tgtccagata gatgcggtcc atggtctccc
      121 cactatagcg aacggtggcg gacttgagca atcgaggccg gagacacccg gcggccgcta
      181 cttcagcttc ttgcggagct tccgcgcgct gggcggcgcc gaggccgggg cggcggcgtt
      241 cagccgggcc cgccgccgca gcacgtagag gaagacgacg gccccggtga tcatcaggac
      301 gttgaggatc tgacccgtgg tgaagttgaa cggcgtgaac tgatacactg gattgtcgac
      361 gtggacgaag cggatgggcc agtcgatccc tttgtccggc tgccggacgt actcgatgaa
      421 gaaccggatg acggcgtagc cgatcacgta gcaggcgatc ccgaacccgt cgtagggttt
      481 gcgctttcgg acgatgaacc acatgatcag ccagagcacg aacccctcga agaacgcctc
      541 gtagagctgg gacgggtggc ggggaagatt caccaggccc gtcgtgggca gcggcatccc
      601 cacgctcctg gcatagtcct ggacccaggg ctggccggag gaaaagctct ccgctccggg
      661 aaacaccatg ccccagggaa cgcgggtgac ccgaccgtac agctccgcgt tgatgaagtt
      721 gccgagccgc ccgaaggtgt accccagcgg gatcccactc accagcatgt ccccccactg
      781 gagcacgctg atcttcttga cccgcaggaa gatcacgatg gcgatggtgg cccccaccag
      841 accgccgtga taggacatgc cggcgatgcc ggtgagccgt atctttccgt ccaccaccgc
      901 gacgggaagg atgatctgga gggggtgatg cagatagtag ccggtggagt cgtagatggt
      961 caccgcgaag gcgcgagcgc cgaccaggag cccgacgatg ccccagaaga acatatccag
     1021 gacgacgtcc ttctccggct tgatgctctg ctcccgaagc tggtaccgaa agaggaggta
     1081 ggcgatcgcg aaggcgatca ggtacatcag gccgtaccat cgaaccggaa ggaagggcac
     1141 gatctgcggg tggatccacg acggataggg aatgtaggcc agcatgcgtc tctccgcgcc
     1201 ctttcagagc gcctctacga tcttgccctg ggcggggtcg aagcgcaagg taatcatgtt
     1261 cttgggcgtc gtcacctcgt accgcttgcc gcggcactcc aggatggaac cggggaagag
     1321 ggtcccccgc acctccaccg tggcgggaac cggctgacgc agcttctcat gcatggccgc
     1381 caggctgacc ttcagctcct cgacgcgctt gatcagggtt cggcgctgct cctgcatctc
     1441 ccgaaggttc ttgggaaccg cgacgctgac accgccgccc gtcccgctct tggccgcttc
     1501 caccgccttc accacctggc ggagcaccgt atccaggtcg gtgagcttct tctgaagcgc
     1561 gtccatgatc cgctggtccc gcgtgaactt ctcctggagc aggaagtcct ccccgaactg
     1621 gaccacggta tggacgccga cggcggagcc gatgttctgc gccaggacgc ccttgtacgc
     1681 ccgcacccgc ccgcccacca gatggccgcg ctcggtctcc aggatgagct tgccgccgca
     1741 ggtgacctgg cacaggacgc aggactcccg cagcttgacg tccccggtcg ccacgagagt
     1801 cgcctgctcg gcgaacgtcg cggtgaggtc cgttcccgcg cgcaggacgg cgcgcccctc
     1861 gcccttgatc ccggcgccca cgacgatgct cgtggcggcg gacaccagcg ccgcctctac
     1921 cgccttggcg acgtccaggc cctgtcccgc ggcaaccttg aagccggacc ggatggagcc
     1981 tcccacgtgg atcaccccgg ggaagtccac gttgccggtg gtcaggtcca cgtcgttcgg
     2041 cacggtatag gactcgtgga ccgagaggac ggcgccatca cgggtcagct ctccggcccg
     2101 ttccgagtag aaccgcgtgg tcccctccga gtccctgtcc gctcggaccc cgcggccggc
     2161 ctgcagtccc aggcggacct ccgcggagac gggcttgacg acgcccgtta cgtcccagcc
     2221 gtccgcctgc tcggcgatca ggggatggag cgtggcgatc agctcaccct tctcgacgct
     2281 ggagatccgg tcctgggcct tgaagtccac gctgccgtcc cggcgcgccg atgccttgcc
     2341 ggtggccagg tgggcgtgga acgtgatccg ctcatccggg gtggcagcgg gcatcttgcc
     2401 ctgcgccacc tcgaagtccg agagcgcctt accctggttc acgtggtcca tcacctggag
     2461 gagctgcgtc tcgttgatcc cgcggaccac accgcccttc tggatgcggt agcggacgtc
     2521 ctccgcgggg atggggtatc cgtccccggc tggcgggatg tacgttaccg ttgccttcat
     2581 acggtcgtcg gagatcgtca cgcgaagctc cgcatcgcga tgcggccgga cgcggagcag
     2641 gatcaccatg ccctcgctgg ccttatccag gatgccgggc accgtggtaa ccacctcgtc
     2701 ccgcttcatc gccagcccct cgtagatccg gacctggggc gccccggcct tggagaccgg
     2761 aatggcgatc ccgaagacgt cgaggccagg ggttccgccg acgccgggag agacccgaag
     2821 aacgggcgcc ccggctgcca cctcggccat ggcctccacc ttctccaggg ggaatgcctc
     2881 cagcgagggg atcttgggaa tccgcttcac atccagcatg acgatgcgct gacgggtctc
     2941 ggcctgggac aggaacggga cgatccactc gatctgcgct tccttgccgg tcttgggggg
     3001 tttgccggtc accaggggat acccggtcag ttccatatcc gtgccggcga aaaacgtcgt
     3061 gagggtcttg cgaagctcct ccgcgttgaa ccccgctacc ctgctggccc ggatggcctg
     3121 gcccacgtcc gcggcggtca ccgtcttgcc ggctccccgg ccctttcgga tgttcagcgt
     3181 cgcggagagc tggtcctccg atatgacgac ctccaccgat ccgtccttcg aaagagagat
     3241 cggcttgcgc gccacgggag cggcgccgga gagcgcgcca tggatcaggg cctcgatctg
     3301 cggctccggc atgagcgtac gggaggcgaa tcccaggcgg ttggcggcct ccaggacctc
     3361 gtaggcctcc gggggcggag tgccgggatc tcccggggtt acggtcagga ggcaggtgag
     3421 cttgtccaag gaggcttcca ccttgacctc gtgatcgttg aagggcacca cctcggccca
     3481 ggcggcgccg cggcgcagga agccggtaac ggtcgccttt acctcggtcc cggaacgctc
     3541 cagaccgtca cagaagagca ccgcgtcctc tccgggacga gccgccagga tgctcctgcc
     3601 gaagacgctc tttccttcct tgccctgttt gccgggctcc acgcgcgcca ccacgcgacc
     3661 gggcgtcgcg tagcccaggg cggtgaccgc cagatctacc gttgccggtt ccttgaccgt
     3721 cttcttctcg acgacggtct ccacggtttc cttggaggca aggaatcctt tcttgatgac
     3781 cttcttctcg cgcttgacct tgacttcccg cagccggtac ggcttgggct ggcccgcttt
     3841 ccccaggacg gcacgggcgc gctcggagag acgcgacggg atggggaggc tctcgaaggt
     3901 gaccttctcc gcgacggtcg gctcgggtgg aatcccgatg gccgcgacga agctcacggg
     3961 ctcggggcgg gagcgattca gcgcgcggaa cgcatgctcg atcgcctgct cgtcgattcc
     4021 gaaccgaacc ttcttgccgg ccaggacggt atgcagcacc tcggggctca actcggcccc
     4081 gcccgcgtcg ggctggatcg tgacgcgtac ttctagcccc aactcatcga tctcgagtct
     4141 gaggtcccct cggatggaca tgagctcctc aatagacctc caccctgaag tgtagagcaa
     4201 cggtgatcag gctgcaaggg caaccgtttc ggatgtttcg ggcgcccgtc ccgggatgag
     4261 agcgccctac gcggaatccg ccggtcgcca ggccatcagg aaggcgagaa acagagcgtc
     4321 caccaggaag atcgagtagg ctacgcgatc cccgttcacc ctcacccggc ccacgaccag
     4381 aagctggatc gactgcgcgg cgcgcagcgc gcgcgagagc acgagcagga ggaacgtgaa
     4441 cgtgccgagg agcttcccca gccgcagcat cccgatcaga gcctcgtagc gccgcggaaa
     4501 cagggacagc agcagcgcgc cggccgccac caccaggctc gggctcccga tcagcaggag
     4561 ccacggatag accccaggcc cggagacgcc ggaggcgtcc aagacgagcg cgacgagcat
     4621 ggtaagaagg aagaacctcg ccagctccca gaaggaaccg gcaagcaaca gggcccttcg
     4681 cgctgtcatc cgtgcggcca gtgtgccacc ggcccggcgg ccgggtcaag gctggacctg
     4741 gaggcgctgg ggcggagcct ccctagcggc tgggcgtccc tgccggcaac cccggcaacc
     4801 caattttcgt tgcaaaagcc cggaactcgg gttacactgc aagacgactg gagaccccat
     4861 gaagcttctg cagacccgcg aacgcgtggt ctggactgcg gtgaccgcgg tcctcctcgc
     4921 ggcgttggcg ttttttgccc tctcacctcg tattctggcc ggaacttcgg atgatgaaac
     4981 ccaagcctac ctgggcaccc ttggggacac gttccgctac atccgggaca attacgtgga
     5041 ccaggagaag gcccagccca aggcgctcta cgaaggcgcg ctcaaaggga tgttcgagtc
     5101 gctgggggac cccaactccg cctaccatac ggccaaggaa tggtcccgcg tcacggacac
     5161 caccacgggc tcctttggcg gcgtcgggct ggtgatctcc acggagaagg ggctcggcgc
     5221 gcggatcgac tccgccatcg agggcacgcc cgcctatcgg gccggcctca ccgccgggga
     5281 cctcatcctc aagatcgacg ggaagccaat ggcggacgtc gagctcaacg acgtggtggc
     5341 ccaactgcgc ggcgtccctc attcgacggt cacggtcacc gtgcggcggg gagaggcggc
     5401 gcagttcgac gccgtcatga cccgagaggt gatcgaggtc ccgacggtga agtacgcggt
     5461 gatgccgggc ggcatcggct acctcaggat cacgcagttc accgcgcaga cggccgaacg
     5521 cacgcgcgaa gccatccgct ccttcgacga cgccgggtac acgtcgctga tcgtggacct
     5581 ccgtcagaac ccgggcggtc ttctcaccgc ggcggtggac gtcgcgaacc ttttcatcga
     5641 ccagggtctg atcgtagaga ggaagaacaa tcgcgtggca agcgagaacg tccgctacag
     5701 cgcgcgtcgc aaccgtcaga tggtggcgcc gaccatcccc atcgcggtgc tggtggacgg
     5761 aggctccgct tccgcctcgg agatcctttc cggcgcgctc cgagactatc accgcgcgtc
     5821 cctcttcggt acgcgcacct acggcaaagg atccgtacag acggtgcagg cgctcggaga
     5881 cggcggcttc cggctcacga cctcgcgcta ctacacccca tccgggatct gcgtggacaa
     5941 ggtgggtctg cagccggaca tcgaggtgaa ggagcccgat ctgagcgagg accagcagaa
     6001 gatcctgggg gacctcctga acgggagcag cctcaaggac ttcgtcaaaa cgaacccgca
     6061 gcccacggag gcgcaggtgg cggagttcgt gtcggggctc catgcgaacg gcgtcacgct
     6121 gagcgatcgg tacctccgca ggctcgtccg caacgaggtc aaccggacca acaacaaccc
     6181 gcccaagtac gatctggact tcgacatcgt tctccaggcg gccgtgaagg cgctggaggg
     6241 tcaccagatc gcgaccgggt cggcgcagta gcgccgcggg gtcggccgtg ccgctacggc
     6301 cgccggccgg ggaggcgtcg ccatgagaca gtacctcctg cccgcctcct acgccggtca
     6361 gccgaccttg accgtgagcg gaaacgactg gcgccacctc acgcgcgtgc tgcggctccg
     6421 cgtcggagac acgctcgcgg cgcgcgacgt tcacggcacg cgctaccgga tgcgcatcgg
     6481 gagcatgggg aggacctcgt gcgtggtcga gctggcgccg caagaggcgc cggcggacgc
     6541 gtcgcccgct cccgcccaga cgggaccccg tctcacgctg cttcagtgct tgccaaaggg
     6601 ccggaaaatc gatctcattg ttcgccaggc aaccgaggca ggcgtctccc ggatcgtacc
     6661 cgtattgagc gaacgctccc tcgtgcgggc aggggaagaa cggagcgccc gccttcgcag
     6721 gatcgccgaa gaggcgatgg agcagagcgg ccgtgaccga ctcccggaca tcgaggaccc
     6781 gcgcacgctg gagtccatct cccgggacca ttgggggacg ggcatcctgt tctacgaatc
     6841 ggcggcgggc gccttgccgc tgcatcatct gctggcccat ggtcccgcgg aggtctgcat
     6901 cctcatcggg cccgagggag gcttcgccgc cgccgaggtc gagctcgtga ccgaagcggg
     6961 atttcgcatc gcccacctcg gggagacggt gctccgggtg gaaaccgccg ccctctatgc
     7021 catcggggcg gtcatgacga tcgtgcagga gagaaacgat tggaaatcgg taccgcggcc
     7081 ggagtgaagc gcatcaaggt gctggacgtt cccgtggact gcgtggatcc ggacgtggcc
     7141 gtccgggtgg tggagtccct gctggacaac ggactgcgca accagatcgt gttcctttcc
     7201 cttcgcgcgc tcatgcgggc gcgacacgac cccgagctgc ttcggtcctt gagggacgca
     7261 gccctggtcc tgccggtgtc cctcgcggtg gtccggggcg cgcgtttcct caaggccgga
     7321 cacccctgcc tgtacaacag cttcgagtac atcatccgca tcctgagcgt ggtggagaag
     7381 gcgaaggggt cggtctatct cctgggctcc cgcaagcaga ttctggaggt agccgaagag
     7441 aacctcttgg gctccttcca cgggatccgc gtggtcgggc ggttctacgg gtatttcccc
     7501 cggaccgtgg aagcggacat cgtcacggcc atcaagaagg cctcgccctc tctggtcctg
     7561 gtggggaccg gcgtgccggg acgcgacaag tgggtcctca agcacaagcg ggacctcaac
     7621 cccggcatct acctgtgggc cgggtactgt ttcgagatct tcgcgggcaa ggagaggcag
     7681 gtctctcgca agctgcacgc caggggactg agcgccttgt cggggatccg ccgaagaccc
     7741 tggcgagtcg tcggcatctt cccctatctc tactaccagg tcctgctcgt ggggtaccgc
     7801 ctcttcaagc tgtaggcgcg cgcgccgcta tactccggaa agcttcagat actcgtagac
     7861 ctcgcggaag tgtgagtcca gcttccgggc gatcaccttt gccaggcggt cgcgaaggaa
     7921 cggcagcagg agaaggtacc ccaccacatc ggtgacgaag ccaggcgtga gcaggagcag
     7981 actgccgatg aggatcccca cgaggtcgac gaactccttc cccggatagg cgctctcacg
     8041 gatcctggcc ttgaggcgat ccagg
//
LOCUS       DASRZS010000127         3009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5456056, whole genome shotgun sequence.
ACCESSION   DASRZS010000127 DASRZS010000000
VERSION     DASRZS010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5456056"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VFH83_03060"
     CDS             complement(<1..935)
                     /locus_tag="VFH83_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6485371.1"
                     /translation="MPGKRIRVGVIGYGGTFDMAKVHLGEMRKAGMSPVAVADIDLER
                     LSVARRDFPGIRTYPSARDLLQDSELDLVTIVTPHDTHARLALQCLRAGKHVVCEKPM
                     AITTAECDAMIRAARERKLLLSTYHNRHWDGWILNAMKLIRAGRIGDVVRVDGYMGQW
                     AKPGDWWRSSKSISGGILYDWGVHLLEYTLQMVDSSIVEVSGYARSGVWAKGNAWNKD
                     TIEDEGFIVVRYASGAWSTLLVTALDAAPRAGWFHVTGTRGTLEFDSQGWKLTTVPDA
                     GRGAPVTATLSGRNPDSEWWRFYKNIAAHLTRGEKL"
     gene            complement(962..1609)
                     /locus_tag="VFH83_03065"
     CDS             complement(962..1609)
                     /locus_tag="VFH83_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HET6485372.1"
                     /translation="MKALVVWGGWDGHEPKQCVDIFAPWLRAQGFDVTVSNTLDSYLD
                     SALMGSLSLIVPVWTMGTISREQEQALLGAVERGCGIAGWHGGMGDSFRQNTDYQYMV
                     GGQWVAHPGGIVDYRVKITKPADPIVAGLSEFAMHSEQYYMHVDPANEVLATTTFGGD
                     IHPWIKGTVMPVVWKRLYGKGRVFYTSLGHVAADFSVPEAKTIVQRGLLWASGSR"
     gene            1852..>3009
                     /gene="glmS"
                     /locus_tag="VFH83_03070"
     CDS             1852..>3009
                     /gene="glmS"
                     /locus_tag="VFH83_03070"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET6485373.1"
                     /translation="MCGIFGYVGAGANPEVLIEGIKRLEYRGYDSWGVCVACDDQLTM
                     LRRVGRIGGVDAADLDLGGKGCLSGIAHTRWATHGAPTEANAHPHADCTGKIALIHNG
                     IIENYGSLREKLRAQGHRFRSETDTEVIPHLIEELLKSEPSFDAAFQGALRLLVGAYG
                     IAAMWAEDPGAIYVARHGSPIVLGLGKDRTLVASDPAPLVAHTREVIYLDDGELAILH
                     CDGFETRTLEGASVNKRVQQIAFGLPDIERGGFPHFMLKEISEQPESIHNAYRGRIVR
                     AEGTAKLGGVDEALLRKVQRCHIVACGTSWNAGLIGKYLLENLARVPTQVSYGAEFRY
                     SHPVIEAGTLVIAISQSGETADTLAAVREAKIVGADTIGVCNVVGSTIAREC"
BASE COUNT          495 a         1045 c          972 g          497 t
ORIGIN      
        1 agcttctctc cccgggtcag atgggccgcg atattcttgt agaaccgcca ccactcggag
       61 tccgggttcc gtcccgagag cgtcgccgtg accggcgccc cgcggccggc gtcgggcacc
      121 gtagtgagct tccagccctg actgtcgaac tccagcgtgc cgcgggtccc cgtcacgtgg
      181 aaccatcccg cgcgcggcgc cgcgtccagg gccgtgacca gcagcgtgga ccaggcgccg
      241 ctcgcgtacc gcaccacgat gaacccctcg tcctcgatgg tgtccttgtt ccacgcgttg
      301 cccttcgccc agacgccgct tcgggcgtag ccgctcacct cgacgatgga agagtccacc
      361 atctggagcg tgtactccag caggtgcacg ccccagtcgt agaggatccc gccggagatg
      421 ctcttgctgg atcgccacca gtcccccggc ttcgcccatt gtcccatgta cccatccacg
      481 cggaccacgt cgccgatccg gccggctcgg atgagcttca tggcgttcag gatccagccg
      541 tcccagtggc ggttgtggta cgtggacagg agcagctttc gctcccgagc cgcccggatc
      601 atggcgtcac actccgccgt ggtgatggcc atgggcttct cgcagaccac gtgcttgccg
      661 gcgcgcaggc actgcagcgc caggcgggca tgcgtgtcgt ggggcgtcac gatcgtgacc
      721 aggtccagct cggagtcctg aaggaggtcc ctcgcggacg gataggtgcg gattcccggg
      781 aagtcccgtc gggcgaccga aagccgctcc aggtcgatgt ccgcgaccgc aacgggcgac
      841 attcccgcct tgcgcatctc cccgaggtgg accttcgcca tatcgaaggt cccgccgtac
      901 ccgatcaccc cgacgcggat ccgcttgccc ggcatgatac ccccgtccgg ccgaccgctg
      961 ctcagcgaga gccgctggcc cagaggagac cgcgctggac gatggtcttc gcctccggca
     1021 ccgagaagtc cgccgccacg tggcccaggg acgtgtagaa gacgcggcct ttgccataca
     1081 gacgcttcca gaccaccggc atcaccgtgc ctttgatcca gggatggata tccccgccga
     1141 acgtggtggt cgcaaggacc tcgttcgccg gatccacgtg catgtagtac tgctcggagt
     1201 gcattgcgaa ctccgagagt ccggcgacga tgggatccgc gggcttggtg atcttcaccc
     1261 ggtagtccac gatcccgccc ggatgcgcga cccactgccc gcccaccatg tactggtagt
     1321 ccgtgttctg gcggaaggag tcgcccatgc ccccgtgcca gccggcgatt ccgcagcctc
     1381 tttccaccgc gcccagcagc gcctgctcct gctcccggct gatcgtgccc atggtccata
     1441 ccggcacgat cagggaaagg gagcccatca gcgccgaatc caggtaggag tccagggtgt
     1501 tcgacacggt cacgtcgaag ccctgcgccc gcagccaggg cgcgaagatg tccacgcact
     1561 gcttgggctc atgtccgtcc cagcctcccc agaccaccaa tgccttcatg ccgtctcctt
     1621 cccgggtaac caccgcgttt ccgaactctg accggattga cccccggatc ctctcccaag
     1681 ggcggtatgt tgtcaagcaa accgcctccg cgcagctccg cgccggcggg ccgtccccgt
     1741 gtgcggcgac gacaacgact gcgggccgcg gcctgaccgg tcgggacttg cgttcggcgc
     1801 acccttctgg tagaagttgc gggaccgatt cgaaaaacgg gagagcgttg catgtgtggg
     1861 atattcggtt acgtaggcgc cggcgccaac cccgaggtac tcatcgaggg catcaagagg
     1921 ctggaatacc gagggtacga ttcctggggc gtgtgcgtcg cctgtgacga ccagctcacc
     1981 atgcttcggc gcgtgggtcg gatcgggggc gtcgacgcgg ccgacctgga tctgggcggg
     2041 aagggatgcc tctccgggat cgcgcatacc cgctgggcca cccacggagc gccgacggag
     2101 gcgaacgccc atccccacgc ggactgcacc ggcaagatcg ccctcatcca caacgggatc
     2161 atcgagaact acggctccct gcgggagaag ctgcgagccc aggggcatcg gtttcgcagt
     2221 gagacggaca ccgaggtcat cccccatctc atcgaggagc tgctcaagag cgagccgagc
     2281 ttcgacgccg ccttccaggg cgcgctgcgg ctcctggtgg gagcgtacgg tatcgcggcc
     2341 atgtgggccg aggacccggg ggccatctac gtggcccggc atggcagccc catcgtcctg
     2401 ggtctgggga aggaccggac cctggtggcc agcgacccgg ctcccctggt ggcccacacc
     2461 cgggaggtga tctatctgga cgatggggag ctcgcaatcc tccactgcga cgggttcgag
     2521 acccgaacgc tggaaggcgc ctccgtcaac aagcgcgtac agcagatcgc cttcgggctc
     2581 cctgacatcg agcggggcgg gtttccccat ttcatgctca aggagatctc cgagcagccc
     2641 gagtccatcc acaacgccta ccgcggacgg atcgtgcggg cggagggaac cgccaagctg
     2701 ggcggcgtcg atgaggcgct cctccgcaag gtgcagcggt gccacatcgt tgcctgcggg
     2761 acgtcctgga acgcggggct gatcggcaag tatctgctgg agaacctggc gcgggtgccg
     2821 acccaggtca gttacggcgc ggagttccgc tattcgcatc ccgtgatcga ggcgggcacc
     2881 ctcgtcatcg cgatcagcca gtcgggtgag accgcggata ccctggccgc ggtccgagag
     2941 gccaagatcg tcggggccga caccatcggg gtatgcaacg tcgtgggatc caccatcgcc
     3001 cgcgagtgt
//
LOCUS       DASRZS010000128         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5456606, whole genome shotgun sequence.
ACCESSION   DASRZS010000128 DASRZS010000000
VERSION     DASRZS010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5456606"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="VFH83_03075"
     CDS             <1..1232
                     /locus_tag="VFH83_03075"
                     /inference="COORDINATES: protein motif:HMM:NF036809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HET6485374.1"
                     /translation="RDHPCLALWCGNNEDLGALTWFPASRQNRDRYVVDYDRLNEGTI
                     GRTLDECDPTHPFWPSSPSGGRGDYSDNWHSDGRGDMHFWSVWHEGKPFEAYRGVRPR
                     FCSEFGYQSFPSLATIRGYAAEEDLNVTSPVLEHHQRNPRGNSLITEMFTRLFRFPEG
                     FPNFVYLSQVQQALAIKTAVEYWRSLRPHCMGALYWQLNDLWPVCSWSSLEYGGRWKL
                     LHYAARRFFAPTLLLCYRTEAGLDVWAVNDQREPVKAKARVSFMSFGGKELKGDSIPL
                     SIPAGKSVLVRRYRSDALPADDQAFVRLTLKEGNRVSTNELFLAEPKRCALARALVQA
                     EVVSDGQGLSVVVSSDAPAFYVTLETEGIEGEFEDNGFSLVPGERRSLAFHPRQPTTA
                     AQLRRALGVRHLRDTYS"
     gene            1295..1759
                     /locus_tag="VFH83_03080"
     CDS             1295..1759
                     /locus_tag="VFH83_03080"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HET6485375.1"
                     /translation="MNAEYANIFVGSAVRIFEQEMGSKLSRKSLTLKNAPVPGLPVSI
                     IIGVTGYLTGQVVYSMDEDFAFKVTRALMPNRLPAEAKKFMSSAVAEVANIITGQATI
                     SLAGEHETIHLTPPAVLTGKDLVVDFLALPTICLSLISEFGVLEINIAIAEK"
     gene            complement(1841..2902)
                     /locus_tag="VFH83_03085"
     CDS             complement(1841..2902)
                     /locus_tag="VFH83_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485376.1"
                     /translation="MKDSLALLSEACAGRRPARPPLFDILQNDSVIEHFSGRRLDGSA
                     DWDVMIQATAAALDGTRSLAAPNRLGEQWKDEVGNVHEAVRWTSWVKQHAFTAAEQWL
                     SWIPGHIERLDARSRPAPAEQRNERARQQELVSHLDGGFYIHCTPSTAINTVMFGYRC
                     GLDALPFLWADEKELIQRWMRAVMSETLRYIELTAHAETGPLAMIYSDVAYHGKLMFS
                     PSMMREMGFFEDVEAICDACHRRGLTVIFHSDGYIMEILDGLVSCGIDGLNPLEKAAG
                     MDIYELRRRYPRLILVGGVDVSRLMPFGTPQEVRQETRRIIEETGAEGRLLIGSSTEI
                     WDDIPLANYLAFHDEAMKG"
     gene            complement(2899..>3098)
                     /locus_tag="VFH83_03090"
     CDS             complement(2899..>3098)
                     /locus_tag="VFH83_03090"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HET6485377.1"
                     /translation="FSQRLGRGIGVLFRLSRESVTRAARAGMTAEEVEAALSRGSRSP
                     LPPNVEHEIKDWIRVVREEES"
BASE COUNT          514 a         1055 c         1003 g          526 t
ORIGIN      
        1 tgagggacca cccctgtctc gccctgtggt gcggcaacaa cgaggacctc ggagcgctca
       61 cctggtttcc ggcgtcccgc cagaaccggg atcgctacgt ggtggactac gatcggctca
      121 acgagggaac catcggcagg acgctcgacg agtgtgatcc cacgcacccg ttctggccca
      181 gctcgccgtc cggcggacgg ggggactact ccgacaactg gcacagcgac ggccgcgggg
      241 acatgcactt ctggagcgtc tggcatgagg gcaagccctt cgaagcctac cggggcgttc
      301 ggccgcggtt ctgctccgag ttcggctacc agtcgttccc gtccctggcc accatccgcg
      361 gctacgccgc ggaggaggac ctgaacgtca cctcgccggt gctcgagcac caccagcgca
      421 acccgcgcgg gaactcgctc atcacggaga tgttcacccg gctgttccgc ttccccgagg
      481 ggttccccaa cttcgtctat ctcagccagg tccagcaggc gctggcaatc aagacggccg
      541 tggagtactg gagaagcctg cgtccgcact gcatgggcgc cctgtactgg cagctcaacg
      601 acctgtggcc ggtatgctcg tggtcttcgc tggaatacgg cgggcgatgg aagctcttgc
      661 attacgccgc tcggcggttc ttcgccccca cgctgctgct ctgctaccgc acggaggcgg
      721 ggctggacgt ctgggcggtg aacgaccaga gggagcccgt gaaagccaag gcgcgggtct
      781 ccttcatgag cttcggcggc aaggagctga agggcgactc catacccctc tccatccccg
      841 cggggaagtc ggtgctggtc cgtcggtatc ggtcggacgc tctgccggcc gatgaccagg
      901 ccttcgtccg cctgacgttg aaggaaggaa accgggtctc gacgaacgag ctcttcctgg
      961 cggaacccaa gcggtgcgcg ctggcaaggg ccctggtcca ggcggaggtc gtctcggacg
     1021 ggcaggggct gtccgtcgtc gtctcctcgg acgcccccgc gttctacgtg accctggaga
     1081 cggaaggcat cgagggcgag ttcgaggaca acgggttctc cctcgtacct ggtgagcggc
     1141 gctctcttgc gttccatccg cggcagccga ccacagcggc gcagctccga agggcgctcg
     1201 gcgtccgtca cctgcgggat acgtactcgt gaccgacggt cggcccgcga gcgccggcgt
     1261 gctatactag accgtaccgg agggagaggt tcctttgaac gcggagtacg ccaatatctt
     1321 cgttggaagc gcggtacgga tcttcgaaca ggagatgggc tccaagctct cccgcaaaag
     1381 cctgacgctg aagaatgccc cggtccccgg gttgccggtc tcgatcatca tcggcgtcac
     1441 gggctacctg acggggcagg tcgtctactc catggacgag gacttcgcgt tcaaggtcac
     1501 ccgggccctg atgcccaacc ggctgcccgc ggaggccaag aagttcatga gcagcgcggt
     1561 ggccgaggtg gccaacatca taacgggtca ggctaccatc tccctcgccg gcgaacacga
     1621 aaccatccat ctgaccccgc ccgccgtgct gaccggcaag gatctcgtcg tagatttcct
     1681 ggcactcccg acgatctgcc tgagcctgat ctcggagttc ggggtgctgg agatcaacat
     1741 cgccatcgcg gagaagtaac aacccggggc gcgtagcccg gggcgcgtag cccggggcgc
     1801 gtagcccggg gcgcgcagcc cgcggtcgcc caagcccccc ttaccccttc atcgcctcat
     1861 cgtggaatgc caggtagttg gccagcggga tatcgtccca aatctccgtc gagcttccga
     1921 tcagcagccg tccctcggct cccgtttctt cgatgatccg ccgggtctcc tgccggacct
     1981 cctggggcgt tccaaagggc atcagcctgg aaacatccac gccgcccacc aggatcagcc
     2041 gagggtagcg ccgccgcagc tcgtagatgt ccataccggc cgccttctcc agcgggttca
     2101 ggccgtcgat cccgcaggag accagcccgt cgagaatctc catgatgtag ccgtcggaat
     2161 ggaagatcac ggtgagacct ctgcggtggc aggcatcgca gatggcctcg acgtcctcga
     2221 agaaccccat ctcccgcatc atgctcgggc tgaacatcag cttcccgtgg taggccacat
     2281 ccgagtagat catggccaga ggccctgtct ccgcatgggc ggtcagctcg atgtaccgca
     2341 gcgtctcgct catgacggcg cgcatccagc gctggatcag ttccttctcg tccgcccaga
     2401 ggaagggcag ggcgtccagc ccgcagcggt agccgaacat caccgtgttg atggccgtgg
     2461 atggcgtgca gtggatgtag aagccgccat ccagatggga gaccagctcc tgctggcgag
     2521 cccgctcgtt ccgctgctcg gccggggcag gccgggaccg cgcgtccagc cgctcgatgt
     2581 gaccggggat ccacgacagc cactgctcgg cggcggtgaa cgcatgctgc ttcacccagc
     2641 ttgtccatcg gacggcctcg tgcacgttgc cgacctcgtc cttccactgc tcgccgagcc
     2701 ggttgggcgc ggccagggat cgcgtgccgt ccagggcggc ggccgtggcc tggatcatca
     2761 cgtcccagtc cgcgctcccg tccaggcgcc ggccgctgaa atgctcgatc acggaatcgt
     2821 tctggaggat gtcgaagagg ggcggccgcg cgggacgacg gcccgcgcat gcttcggaaa
     2881 gcagagccag ggagtccttc acgattcctc ctcccggacg acccggatcc agtccttgat
     2941 ctcgtgctcg acgttgggcg gcagaggact gcgggatcct cgggagaggg ccgcctccac
     3001 ctcttccgcc gtcatgccgg cgcgcgccgc gcgggtgacg gattcccggc tcaggcgaaa
     3061 gagcaccccg atgcccctgc cgagccgctg gctgaaca
//
LOCUS       DASRZS010000129        23515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2762236, whole genome shotgun sequence.
ACCESSION   DASRZS010000129 DASRZS010000000
VERSION     DASRZS010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 23515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23515
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2762236"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..3077)
                     /locus_tag="VFH83_03095"
     CDS             complement(123..3077)
                     /locus_tag="VFH83_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HET6485378.1"
                     /translation="MGSIDSVEAGFQRTAALVSYERVRFASQAAFAAELAAQDRAHGR
                     EWTALIDKASALVAAAAQRGDAAALRDSVRNAEQLMAPIGPAARAFTIYCVGHAHIDM
                     NWMWSWPETVSVSVDSVTTVLRLLEEQPQFRFSQSQASVYAILERHRPDLLERVKRQV
                     KEGRWEVTASHWVETDKNMASGESLSRHLLYTRRYMKSLFGLEPEDVPVEWSPDTFGH
                     AATVPTYLVRGGIRYVYLHRPGVHAGTGPAPERPRAFWWVSPDGSRVLVRNDMALGYN
                     GAIGPEMVPRSLIPFWKATGLPMTLFVYGVGDHGGGPTRRDLDRLVDMARWPIYPTVR
                     FSTAKEFFQRLEKEGASLPSIQGELNFEFAGCYTTQTLVKKANRFAEKRLGGAEAASL
                     FAQRLASVPYPTLGLEQAWRDTLFSHFHDILPGSGVRDTRTYTHGLYQQVVAFTSSAQ
                     TQALRAIADLVDTGVEAGPASTAMEAEAFAGPTEGGSGAGFASVDGGISGYVSGPGKG
                     VHALVVFNTTEVERTEIAEATIWDPGWGWEQAAPDAIRFEVEGPDGKKVSAQVLSRDA
                     YWGHAYQRIAFPLTVPGLGYSRILVHEQTATAVERQVTVEPPRATPATAARRTAFPHA
                     CFYSAYERSPDGLENDHLSLELDMRSGGIKSLKHLPTGTVLIQDAAAIVQYAMERARS
                     MSAWLIEHEGRITLPVVTTVEPVQSGPYVASIAVSLKVASSDLKLTYELRAGDPRLHL
                     YLQVTWMERGGPDVGTPVLRLALPFSFASAAKGTRVHYEIPFGSIERTLQHGEEVPAL
                     RWAAVEAGDPGKRVTCVLFNDSKYGHSFLDGELGLTLIRSSFNPDPLPEIGQHEVRCA
                     LFCMPAPFDAAAASRAASAFDQELAVVSTSRHPGRLAPTAGLVRVEGGVLSGLKAAED
                     GAGVVARLYNPSDAPVTARLRFAAELGKVMRAEENDLIERVGEALPVQGDTVNVPIGA
                     RALRSVRVLLSP"
     gene            3216..4217
                     /locus_tag="VFH83_03100"
     CDS             3216..4217
                     /locus_tag="VFH83_03100"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HET6485379.1"
                     /translation="MVLAVFYTLLQLLLLSALGFGLSRFRGYPRVLFQGINRLTLNVG
                     LPVYFFVSISKTSVADIRSGWIFPIAAACLIGTNLAISAPLFRLFRFEEADRRAGMAL
                     SAFGNSSYIPLALMELFPITIPAIAQRFSALTASVYVGTFLLVNSPVQWTLGNWLVAG
                     QGRRPRVTELVTPPFIGIVAGLAVVLLGIQPILFDPSLPLLYAYKAMEKIGSLTLPLV
                     MVCLGAMIADLPFDRESGREMIGMALGVSAVRYLAMPLLFVALYLVVLRPAGITPVQT
                     WVIFLEMHVPPATNLTIMAARAGRNEHHVSFTLLFTYLAYVLVLPVFLLVFLRLTGT"
     gene            complement(4231..5169)
                     /locus_tag="VFH83_03105"
     CDS             complement(4231..5169)
                     /locus_tag="VFH83_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485380.1"
                     /translation="MKETAVAIGTGTHRYQVLDGWAKLPSSLTFGTTHGVVEDSQGRI
                     FIHHTGKQSVVLFDPDGNFLSSWGDEYQGGAHGMFLSREPDGEYLYLSTTGLGFMAKT
                     TLDGREVFRIGTPDRPDIYDAERKFVPTETAVAPDGRIYVADGYGQPWVHVYSKDARY
                     IKSFGGKGDGKGQLDNPHGIKIDTRGPQPLVQVSDRGNSRLQYFTLDGEHVRFVQGDL
                     RKPCTTYQWRDEIYIPDLYSRLTILDRQDRLITHLGDWVDCWKKAGWPNLPRSQWVSG
                     KFSSPHDLHVDAKGNIYVVEWLSEGTGKVTKLLRER"
     gene            5477..6121
                     /locus_tag="VFH83_03110"
     CDS             5477..6121
                     /locus_tag="VFH83_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485381.1"
                     /translation="MKPFVVLVVVVAAAFLLFACTSVAPKAAPQAPAATPEAAAPQAA
                     PEAAAPAAAPEAAAPQAEAPEATAPEAAPQAAAPEAEAQPTAPQEAAPVSVWQATGVT
                     VTKDGDTVDLPPEFTSYPVTARFLDDSHLELSVGEVGFAIPSTYSLDGDNLAISPVDY
                     ENPPQLLIDTMSDMGITELPQTYYFTVSEAADGSGILQADAMGYHMVATWTQVQ"
     gene            6223..7050
                     /locus_tag="VFH83_03115"
     CDS             6223..7050
                     /locus_tag="VFH83_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HET6485382.1"
                     /translation="MNRSVVALLTDFGTEDVYVGVMKAVILSEAPETHLLDLTHQVAP
                     GDVVGAAFRLWQAAPYLPKGVVVLAVVDPGVGTRRRAIALELDGLRCVGPDNGIFTYL
                     LATRRLSALVELTPPPSGVSNTFHGRDLFAAAAARLCSGSPLKRLGEPAADPVLLPLP
                     RLSVDRASIGGEIIAIDRFGNAITSIGAIRTAEEGIALTPWLPGCAEARLSGIGFHVT
                     LSTGESVPLARTFGDVPKGDALTYVGSDGLMEIAVNGGRAADVLGISIADPVELQAL"
     gene            complement(7047..8213)
                     /locus_tag="VFH83_03120"
     CDS             complement(7047..8213)
                     /locus_tag="VFH83_03120"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485383.1"
                     /translation="MTERERILCVLEGGKPDRPPWATRLDLWLEARARAEPRKDHRVS
                     VMSIYRDLGVGRQSYVQITVNRLRGVELLADLDGVPFLRERDPLLRFPLVTDLVPRNR
                     PGVSTFTFTTPVGDCRIQYTTTPEILATGTLPFLTRRSIAEEADFAAVFWILDRLDVL
                     PEYAPLLQREEEIGGDGLAIGMIDRLPFQRVLLDFLGEERTFFEMYDEPSRFSRLLDR
                     LEELRTQSVAVAAASPAVMVEHSDNVDGEMTNPRLFARYCMPALQRSAEAVHGSGKHL
                     GSHMDGDLSALLDLVPESGVDVVESFSPEPLSRLSFRRAWERWRDRVVLWGCLASPRF
                     ESQSPTHLLVSETREVLSRARSAGRIILGIADQAVGPTLEERIRLVTGMVTQQG"
     gene            8426..9655
                     /locus_tag="VFH83_03125"
     CDS             8426..9655
                     /locus_tag="VFH83_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET6485384.1"
                     /translation="MAKVSTLKKARGGSPRKAAAAKTAKPKTRRAAAFAAARLRPVEL
                     LIGTIKGGFALKGSDPGRSWSLTGPYFLGCEVNDLVLDPWDRRTLLMAAKTGHLGPTV
                     FRSKDQGKSWQEARQPPAFPKEPGGKAVDRVFFVTPGHPSQPGVWWAGTVPHALFRST
                     DGGESWAPVQGFNQFVAGLKERTPNLLGETPGGAITHSLLIDPRDAAHMYVSLSTGGF
                     FESRDGGQTWKPMNQGSIAVFLPDPPPEYGQDPHWAVLCPADPDRLYQQNHCGVYRLD
                     RPGEKWDRIGRNLPKEVGDIGFPIVVHPRDPDTVWVFPMDGTDVWPRTSPAGRPAAFR
                     SRDGGATWTRQSRGFPSLGWFSVLRQAMKCDANDPLGLYLGTTSGEVWVSRDEGATWS
                     RVAEHLPRILSIQVAAR"
     gene            9652..9918
                     /locus_tag="VFH83_03130"
     CDS             9652..9918
                     /locus_tag="VFH83_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET6485385.1"
                     /translation="MSVPVHVSTHLHSYTGGLSTIEADGATLAELLVDLDRRYPGLRF
                     RVVDEQDRVRRHMNFFVGGEMTRSLDQRIEPNQEVHILGALSGG"
     gene            complement(9929..10513)
                     /locus_tag="VFH83_03135"
     CDS             complement(9929..10513)
                     /locus_tag="VFH83_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6485386.1"
                     /translation="MNVRLVDVGYRSTHYYLIQPPGGKRLLFDCGWPGTIGTFRSVLK
                     RNGVSIEDIGHILVSHFHPDHAGLVQEVKDAGALLIVTEAQIPFVKPLERIAKPDSGY
                     LPIRPGDNVVVNPEESREFLKGIGLDGEILSTPGHSPDSVSLILDEGLAFTGDLPHQI
                     LLTPEDREAHQSWARLKDRGVHTVYPAHGPPVSI"
     gene            10602..12200
                     /locus_tag="VFH83_03140"
     CDS             10602..12200
                     /locus_tag="VFH83_03140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6485387.1"
                     /translation="MMVDGHSTSYAWQVHTGDICVLPVGAVEQHGDHLPLVTDGVAAE
                     YVARTVAEHLDAALLPAQLYATSLEHTGFRGSVTLRPETLMQIVRDVSDAMERQSFRI
                     LVLVNGHGGNFSLGPVVRDINRMDRALKILLVNLWEYCDPGIAASSASLGMDIHAGEW
                     ERSLMLALAPEDCGAGPGKDMPVQPSPTLLQRDLNTFGVGHFNPAGSVGFPSRATAAK
                     GKAIVASIRRNLLPALDARIERLRANRRYAGSGGIALRTMGEQDVPAGMGLKSAAGWN
                     QTEADWRRFLSLSPGGCQVAVHNGAVVGTAAVLPFGKRLAWLSMILVAPEMRGHGVGT
                     LLARSALAAASGIPLVRLDATPAGQPMYQSLGFTVEYPLARLFTRSAHRAIEATRPAR
                     RVAGRDLAAIRRLDATVFGADRGPLLESLLGDYPELALQIRRDGRVVGYCMGRHGSEC
                     TQVGPVVAESEQDAEALVHAALRVLAGRAVIIDVPTRHQDFSEHLYGLGFGLERPFAR
                     MSRGKGRLPARIERQYAIVGPEFG"
     gene            complement(12213..12596)
                     /locus_tag="VFH83_03145"
     CDS             complement(12213..12596)
                     /locus_tag="VFH83_03145"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HET6485388.1"
                     /translation="MKRRGRIVLIVVGAIVVVIAIGVIAALNGMSYVRHMSVTPLDVS
                     SASDGTHHGEFTRGRFRFAVDVTVADHKITGVTETDKSANDVTRRIAQQIVSQQSLPV
                     DAVSGASLTTKAFSQAVSNALAAGR"
     gene            12868..13803
                     /locus_tag="VFH83_03150"
     CDS             12868..13803
                     /locus_tag="VFH83_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485389.1"
                     /translation="MGRYILGRIAGLLFTFFMVSVMTFSLMHAVPGGPFDENKQPLPP
                     AAKANILRKYGLDRPIYEQYARYMWAALHFDFGIPFQSPTETVTKLIARVWPATLQLA
                     GLVILLSYSLGMLFGILAAINQNTWIDRGVTTLATLGFTVPNFVIGIWLILIVSVRLH
                     WLPTGGWGSPKQFIMPALVYAQAPMALVARYTRVNMLEAMRADYVRTARAKGMSERTI
                     ILRHVLKNALIPLITVIGPQIPNIATGSIFIEAVFRIPGLGRYFISSTYNRDYPMIMA
                     TMLLAAALWGVTYLISDILYTVIDPRVRLTGRRDA"
     gene            13800..14690
                     /locus_tag="VFH83_03155"
     CDS             13800..14690
                     /locus_tag="VFH83_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485390.1"
                     /translation="MSTPAVSAAPARPAVRRTLWRDATRSFSRNRLAMIGFAVVALFV
                     LVAIFANFVAPQPYYRAKLSENLQFPSIRHLLGTDAVGRDLLSRIIYGIRTSLFVGFS
                     VQIVAFAIGVTLGALAGLRGGWVDFVVTRILEIMTAFPGFLFAIFLMGILGNGVLNVI
                     LAISVTSWIDPCRLTRGQLIALREREFVVAAHSYGSSDWRIAIRHLLPHALPPLVIML
                     ALGIPNAIFAEAGLSFLGVGINDPIPSLGKMVSDSMGYISVYWYMGVFPTLTIALAML
                     SFNYVGDGLRDALDPTMYNR"
     gene            14899..16590
                     /locus_tag="VFH83_03160"
     CDS             14899..16590
                     /locus_tag="VFH83_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6485391.1"
                     /translation="MAALPVTAVLFLVMVIAAPAAMAQVKNAWGVTLPADAAPLNQQF
                     LRVLGLEGTTMDFAVSVYKRPANIDMLSTPMVQINKNFEVVPAGALSYAASADGKTWT
                     FKMDPKLTWSDGNPVTADDVVFTFQYQADPKHAWDFAWFWSDIVNWDDAVAGKVPTSA
                     IGVKKVDTYTVQFTTSVPSPYFPSKALYIRPLSRVAFQKYGEYYDNDPKTSVSSSPWI
                     LQEWTKGVRIVLAPNKKYTGREKPYLQYLIYEIGDLGTEFNAYQNGEIDLAFNFSPAN
                     ITTINNDPQLSKEYHPGYGDFRTYYLGFDTTTKPFSDLRVRQAFAKAIDRDTLIKNVV
                     GRQGMAAYSFLMPGFPDANSKALSQENVNKYDVAAAQKLLADAGYPGGKGFPSLEMWL
                     RTENSFNESIAEAVASMIKDNLGIDVQVSQKEQKLFMDTLNAHKLPFYYLSYGFDYLD
                     PSNMLGIWVTGGRHTWSNPQFDSLIKQASSLSGNPAKRDQEFKDAEKILVDDVGGVFL
                     YHVTPGTVYKPYMKGSELLPDKTGVAAWHWPYEEDASTSLPTIYVTNDVPSDRHM"
     gene            16661..17674
                     /locus_tag="VFH83_03165"
     CDS             16661..17674
                     /locus_tag="VFH83_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008185604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485392.1"
                     /translation="MDQALLDVQGLETRFWTREGAVHAVNGISLRLENGETVGIVGES
                     GSGKSVTMLSVLRLLPTPPAEIAAGSVLFQGRDLLTMSDADIRAVRGRQISMVFQDPM
                     TSFNAVLTIGRQITEPLQVHMGMSRAQAWVRAAELLDLVGIPKAKDRLHDFPHQFSGG
                     MRQRAMIAMALACHPNVLIADEPTTALDVTIQAQIVELVKRLRDEMGMAMVWITHDLG
                     IIAGLAQRVAVMYGGYFIEESPVRDLYADPRHPYTIGLLGSLPRVDVGRRRSLTSIDG
                     RPPVLFEKPRYCPFVSRCRYAIARCSQENPALEEVAPGHRVACWVKPRLEKRDGDGTV
                     GKA"
     gene            17731..18654
                     /locus_tag="VFH83_03170"
     CDS             17731..18654
                     /locus_tag="VFH83_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6485393.1"
                     /translation="MAGTIVRRQVGQIHAVDGVSFDLHRGETLGLVGESGCGKSTTGR
                     TILQLYRPTSGSVYLDGVDLARLNRKELRPVRRTMQMIFQDPYASLNPRLTVGEIVGD
                     PLVVYREGSRRQIQEEVRTLLELVRLDPSYANRYPHEFSGGQRQRIGIARALALRPKL
                     VVCDEPISALDVSIQAQIINLLVELQKQLHLTYLFIAHDLSMVRHVCDRVAVMYLGVI
                     MELADSVGLYQDPLHPYTKALLSSVPIPDPVVEEKRKRVILSGEIPSPAHPPSGCRFR
                     TRCPIAQPVCGETTPPLRELRPGHFAACHFA"
     gene            18711..19205
                     /locus_tag="VFH83_03175"
     CDS             18711..19205
                     /locus_tag="VFH83_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485394.1"
                     /translation="MKTLFKAIVPLSFGFILTLSQCSVLKLDVPGAAGTWSGTWNNTT
                     FNSTGSATMVLTVDSAAKTLSTVTTLGGNVFGQGNNPPAQTVTGTYTDSLITLSSTTA
                     LFGAVTLTIDNMGKISGTAHPSGFDSVSVSGTASPTKLDMTYTIYQPAGTVYATGTLT
                     FTKQ"
     gene            complement(19208..19729)
                     /locus_tag="VFH83_03180"
     CDS             complement(19208..19729)
                     /locus_tag="VFH83_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6485395.1"
                     /translation="MKPRSTRTGARPAVTGGIVGGVHTILYSRKPEEVRAFFRDVLGL
                     ASVDAGDGWLIFAAPPGEIAVHPTDGAARHELYLMCSDVTAAVRRLEERGARMAGPIT
                     DQRWGMLARIRLPDGKLLGLYEPRHPTAVRMRGTPRPAGKKRAAAAKQGSAGKKRAAA
                     AKKRGSAKRTRGG"
     gene            19813..21459
                     /locus_tag="VFH83_03185"
     CDS             19813..21459
                     /locus_tag="VFH83_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485396.1"
                     /translation="MPEAIVRVRVDAAEDLGELPRIWNYIGYDECNYTYVPEGRALLA
                     LLGRLGRPVYVRTHHLFCSGNCHGTPKWGSTNVYREDERGQPVYDWTFIDLVFDTILS
                     SGCRPFVELGFMPRDLADPTRYDAASGDWTASAYRRAGWACPPKDYGRWHELVLRTVR
                     HCLERYGEPEVLQWYWELWNEPDIPYWRGTREEFEKLYDYTAAAVKSAHPHARVGGPA
                     VTNPAPGKPGSEFLSSFLSHTSVGTNHCTGGRGTPLDFVTFHAKGGGYSANASRRKES
                     PPSVRTLVESVVSGHQIIDRFPDYRGLECVISECDPDGWAAGGAWDNVNLDFRNTEYY
                     ASYVACSFDRVLKHGLAKGWKVKALSWAFLFVGERCFEGTRTFTTQGIGKAILNLFRI
                     YARLGGRMVKLESSASQEPRCDPDGEAPVVDGVACLGSGRLTVLLYSHHVDWDRNGST
                     AVSLEAVNLPFGTRRCRVRHFRVDAAHSNAHAEWVRQGQPLYPSTAQRRAIKRREGLE
                     TLEPPRAVALLGGTLKLRFTMPVRAVSLLVITPAVRRGES"
     gene            21461..22252
                     /locus_tag="VFH83_03190"
     CDS             21461..22252
                     /locus_tag="VFH83_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6485397.1"
                     /translation="MRFGVCGSTVVPGSSGVEIIEKAGNAGYDYMELSLSHISALTEE
                     TFGALRARVTDSGVPCEACNNFFPPSVRLTGPEVRWSAVQDYTRLALGRALALGARVV
                     VFGSSGAKNVPPGFPKEKALDQIVSALRFVAEEAGPRGITIAIEPLNRKESNIVNDLT
                     EALALMNTVDRPEIRVLVDYYHFALEGDTPEALLRAGPYLRHAHVARVEGRAFPQEPD
                     AGLRSFFRMLSGVGYQARVSVEGATSDFDRDGPQSLRTLRAMASA"
     gene            22339..>23515
                     /locus_tag="VFH83_03195"
     CDS             22339..>23515
                     /locus_tag="VFH83_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6485398.1"
                     /translation="MKRTTKTVAIAAGVLVVGLAAGMLLAQGRSESLATGRTPTQTAS
                     VQALPVADPSSLQSLQLSFREVAQRIIPSVVEINVTEMVNQPTQDFSFPFPFPFGQQQ
                     PQGGSQRVPVSALGSGIIVQHTGNRYFVLTNNHVVDSATQVSVRLNDQRTYKATVLGK
                     DPLKDLAMVAFDTSDSIPVAALGDSNSLQVGDIVLAVGNPFGYTDTVTMGIVSALGRR
                     GPPGQQPSTYTSYIQTDAAINQGNSGGALVNVDGQVVGINTWIAAPNGGNIGLGFAIP
                     IDTAKTDVQQLATKGQVQYGWMGVEISDIQDSATYPGYATDLKLQGVNGAMVMDVYKG
                     SPAAEGGVLPGDYVTAVDSTPIQNADQLTQVVGNLIAGRTYTFSVLRDGTRTDLRVKI
                     "
BASE COUNT         4031 a         7780 c         7596 g         4108 t
ORIGIN      
        1 tgcaggacga catcgcttcg gtcgccttct ggtatcagac gccgcaggcc gcctccttcc
       61 cgacgctccc ggaccgggac tacctggaga tcatctagga gggagcgggg cgcgcgtccc
      121 gctcagggag aaagcagcac gcggaccgag cgcagcgcgc gggcgccgat ggggacgttg
      181 accgtatccc cctggacggg aagcgcctca ccgacgcgct cgatcagatc gttctcctcc
      241 gcgcgcatga ccttgccgag ctccgcggcg aaccgcaggc gcgccgtcac cggcgcgtcg
      301 ctcgggttat acagccgggc taccacacct gccccgtcct ccgcggcctt caacccgctc
      361 agaacgccgc cctccacgcg gaccagcccc gccgtcggcg ccagccgacc cgggtgccgg
      421 ctcgtgctca ccacggcaag ctcctggtcg aacgccgagg ccgctcggga tgccgcggcc
      481 gcgtcgaacg gcgcgggcat gcagaagagc gcacagcgca cctcgtgctg gccaatctcg
      541 ggcaaggggt ccgggttgaa gctgctccga atcagggtca gacctagctc gccgtccaga
      601 aaggaatgcc catacttgct gtcgttgaag agcacgcagg tcacccgctt gccggggtct
      661 cccgcctcca ccgccgccca acgaagggcc ggcacctctt caccgtgctg cagggtgcgc
      721 tcgatggagc caaacgggat ctcgtagtgc acccgggtcc ccttggccgc tgatgcaaag
      781 gaaaagggaa gcgccaggcg caggaccggc gtcccgacgt ccggaccgcc ccgctccatc
      841 caggtgacct ggaggtacag gtggagccga gggtcgccgg cccgtagctc ataggtgagc
      901 ttgaggtcgg aggaagctac cttcagggac acggcgatcg aagcgacata gggaccggac
      961 tgaacgggtt ccaccgtggt gaccaccggc agcgtgatcc gtccctcgtg ctcgatgagc
     1021 caggcgctca tggaccgggc gcgctccatc gcgtactgta cgatcgcggc tgcatcctgg
     1081 atcaggacgg tgcccgtcgg caggtgtttg aggctcttga tgccgccgct cctcatgtcg
     1141 agctccaggc tgagatggtc gttctccagt ccatccgggc tgcgctcgta cgccgaatag
     1201 aagcaggcgt gggggaaggc ggttcgccgc gcggcggtag ccggcgtggc acggggaggc
     1261 tccaccgtca cctggcgctc caccgccgtg gccgtctgct cgtgcacgag gatgcggctg
     1321 taaccgagcc ccggtaccgt cagggggaag gcgatcctct ggtaggcgtg accccaatag
     1381 gcgtcgcgcg acagcacctg agccgacacc ttcttcccat ctgggccttc cacctcgaac
     1441 cggatggcat cgggagcggc ctgctcccag ccccagccgg gatcccagat ggtggcttcc
     1501 gcgatctcgg tacgctccac ctcggtagtg ttgaatacca cgagcgcgtg cacacctttg
     1561 ccggggcccg agacgtagcc gctgatgccg ccgtccaccg aagcgaaccc cgcgccggaa
     1621 ccgccttccg tgggaccggc gaatgcctct gcctccatgg ccgtcgaggc gggccccgcc
     1681 tccactccag tgtccacgag gtcggcgatc gcccgaaggg cctgggtctg ggccgaggag
     1741 gtgaaggcga ccacctgctg atacagccca tgcgtgtagg tgcgcgtgtc ccgaacgccg
     1801 gacccgggaa ggatgtcatg gaagtggctg aagagggtgt ctctccaggc ctgctccagc
     1861 ccgagtgtgg gatagggaac cgaggccagc cgctgggcga acaaggatgc ggcctctgct
     1921 ccgccaagac gcttctccgc gaaccggttc gccttcttga ccagggtctg cgttgtgtag
     1981 cacccggcaa actcgaagtt gagctcgccc tggatagacg ggagagaggc gccctccttc
     2041 tccagcctct ggaagaactc cttcgccgtg gagaaacgga ccgtcgggta gatgggccaa
     2101 cgggccatgt ccaccagccg atccaggtcc ctgcgggtag gtccgccgcc atgatctcca
     2161 accccgtaaa cgaacaacgt catgggaaga ccggtagcct tccagaacgg aatgaggctg
     2221 cggggaacca tctccggtcc aatggcgccg ttgtacccca gggccatgtc gtttcgcacg
     2281 agcacccggg acccgtccgg ggatacccac cagaaggcgc gaggccgttc gggggcgggc
     2341 cccgtcccgg catgcacgcc gggacggtgc aggtagacgt aacggatacc gccacggacc
     2401 aggtacgtgg ggacagtggc ggcgtggccg aaagtgtcgg gagaccactc cacgggcacg
     2461 tcctcgggct ccaggccgaa cagggacttc atgtagcggc gcgtgtacag gagatgacgg
     2521 ctcaggcttt cgccgctcgc catgttcttg tccgtctcca cccagtggct ggcggtgacc
     2581 tcccagcgtc cctccttcac ctggcgcttc acccgctcca ggaggtcggg ccgatgccgc
     2641 tccaggatgg catagacgct cgcctggctc tggctgaagc gaaactgcgg ctgctcctcc
     2701 aggaggcgta gcaccgtggt cacggagtcc acggacacgg atacggtctc gggccaggac
     2761 cacatccagt tcatgtcgat atgggcgtgt ccgacacagt agatcgtgaa cgcacgcgcg
     2821 gccgggccga tcggggccat gagctgctcg gcgtttcgga ccgaatctcg aagcgccgcg
     2881 gcgtctcccc gctgggcggc ggcagccacc agggcggagg ccttgtcgat cagggccgtc
     2941 cactcccggc cgtgcgcccg gtcctgcgcc gccagctcgg cggcaaaggc ggcctgggac
     3001 gcgaagcgca cccgctcgta gctcaccagc gccgcggtcc tctggaaacc agcttcgacg
     3061 gaatcgatgg agcccatggt gtttcctccc gatgggtctc ttctacgggc tttggtcgct
     3121 cccgtcaagg cgaggacgcg agctcaggcg ccaagccagc tcagccaggc tcgcgttgca
     3181 ccacgagagg ggatccggta gactgcgcgg aatccatggt actggcggtc ttctacacgc
     3241 tcctgcagct tcttctgctc tccgctctcg ggttcggtct gtcgcggttc cgcggctatc
     3301 ctcgggtcct cttccagggc atcaaccgac tcacgttgaa cgtcggactg ccggtgtatt
     3361 tcttcgtgag catctccaag accagcgttg cggacatccg ctcagggtgg atcttcccca
     3421 tcgcggccgc ctgcctgatc ggaacgaacc tcgccatctc ggctcccctg ttccggctct
     3481 ttcgcttcga ggaagcggac cgacgcgcgg gcatggcgtt gtccgctttc ggcaactcga
     3541 gctacatccc gctggcgctc atggagcttt tccccatcac cattccggcg atcgcccagc
     3601 gcttcagcgc gctgaccgcc tccgtctacg tgggtacctt cctgctcgtc aacagcccgg
     3661 tgcagtggac gctgggcaac tggctggtgg ccggtcaagg ccgccggccc agggtcacgg
     3721 agctggtcac gccgccgttc atcgggatcg tggcgggtct ggccgtggtg ctcctcggaa
     3781 tccagccgat cctcttcgac ccgagtcttc ccttgctcta cgcgtacaag gcgatggaga
     3841 agatcgggag cctgacgctg ccgctggtca tggtctgcct tggcgccatg atcgcggacc
     3901 tccccttcga ccgggagtcc ggccgggaga tgatcggcat ggccttgggc gtctccgcgg
     3961 ttcgctatct cgcgatgccg cttctcttcg tggcgctgta tctcgtggta ttgaggccgg
     4021 cgggaatcac tccggtgcag acctgggtga tctttctgga aatgcacgtc ccgcccgcga
     4081 ccaacctgac catcatggcc gcgcgggcgg gacgcaacga acatcacgtg tccttcacgc
     4141 tgctcttcac gtacctggca tacgttctgg tgctccccgt tttcctgctc gtattcctgc
     4201 ggctgacggg gacctgagcc gtgccgcggt ctaccgttcc cgcagaagct tcgtgacctt
     4261 ccccgtcccc tccgacagcc actccacgac gtagatgttt cccttggcgt ccacgtgcag
     4321 gtcgtgcggg gatgagaact tcccgctcac ccactgggac cgcggaaggt tcggccatcc
     4381 cgccttcttc cagcagtcca cccagtctcc caggtgcgtg atcagccggt cctggcggtc
     4441 caggatcgtc agtctggaat acaggtccgg gatgtagatc tcgtcgcgcc actgataggt
     4501 ggtgcagggc tttctcaggt ctccctggac gaaccgcacg tgctccccgt ccagggtgaa
     4561 gtactgcaga cggctgttcc cgcggtccga tacctggacc aacggctgcg gtccacgcgt
     4621 gtcgatcttg atgccgtgcg ggttgtcgag ctgacccttc ccgtccccct tgccgccgaa
     4681 gctcttgatg tagcgggcgt ccttcgaata cacgtgcacc caaggctgcc cgtatccgtc
     4741 ggctacgtag attcggccgt ccggagccac cgcggtctcg gtgggcacga acttgcgctc
     4801 cgcatcgtag atgtccggtc ggtccggagt gccgatgcgg aagacctcac gcccgtcgag
     4861 ggtggtcttg gccatgaacc ccagcccggt agtggagagg tagagatact ctccgtcggg
     4921 ctcccggctg aggaacatcc cgtgcgcccc gccctggtac tcgtctcccc aggatgacag
     4981 gaagttccca tcgggatcga agagaaccac ggattgcttg cccgtgtgat ggatgaagat
     5041 cctgccctgg gaatcctcaa cgaccccatg ggtggtgccg aacgtcaacg acgacggaag
     5101 cttggcccaa ccgtccagaa cctggtatcg atgcgtgccg gtcccgattg cgaccgcagt
     5161 ttctttcaca attcgatcct cccgctacct tactatggta ctcagatttg gatgcacgga
     5221 ccatggcggc cacggaaccc acgcggtact ccgcgtcccc gtggcatttg cggccacgga
     5281 acccacgcgg tactccgcct gctccgcgtg atcttctgtg gacaactgcc ccggacaggc
     5341 ttaagatcgt cgtggtttcc cggtggatga gtccccttcg gccgcgcacg cggcggcttt
     5401 tcgtttgtcc cgaatgggca tccgtcggat gtcttgcaga atgtcttggg ctggagtggg
     5461 aggtatgcaa gtgaaaatga aaccgttcgt tgtgctcgtg gtggttgtgg ccgcggcgtt
     5521 cctgctcttt gcgtgcacga gcgttgcgcc caaggcggca cctcaggcac cggcggcaac
     5581 ccctgaggcg gcagcacccc aggcggcgcc ggaggcagcg gcacccgcgg cagctcctga
     5641 agcggcagcc ccccaggcgg aggcgccgga ggcaacggca ccagaggctg cgccccaggc
     5701 agccgctccc gaagcggagg cgcagccgac ggccccgcag gaggcggctc cggtgtcggt
     5761 ctggcaggca accggggtca cggttaccaa agatggggac acggtggacc tgccgccgga
     5821 gttcacgagc tatccggtga ccgccaggtt cctggatgac tcgcatctgg agctcagcgt
     5881 gggggaggtg gggttcgcga tcccgtccac ctactccctg gacggcgaca acctcgcgat
     5941 ctcacccgtg gactacgaga atccgccgca actcctgatc gacaccatgt cggacatggg
     6001 catcacggag cttccgcaga cctactattt cacggtctca gaagccgctg acggaagcgg
     6061 gatcctccag gcggatgcca tgggatacca catggtcgcc acctggaccc aggtgcagta
     6121 gaggcggatc cgatccggcg gcgctttcgc cgctggccac gactcaggcg gcacgggtag
     6181 ggtggatccg ggccgccttc ccttctctgc tatagtgtct acgtgaatcg atccgttgtg
     6241 gcgctattga ccgacttcgg cacggaagac gtctacgtcg gggtcatgaa ggcggtgatc
     6301 ctgagcgagg cgcccgagac tcacctgctc gacctcacgc accaagtcgc ccctggggac
     6361 gtggtgggcg ccgcttttcg gctctggcag gcggctccct atcttccgaa aggggtggtt
     6421 gtgcttgccg tggtggaccc cggcgtcggc acccgacggc gcgcaatcgc cctggagctc
     6481 gacggtctga gatgcgtcgg gccggacaac ggcatcttca cctaccttct ggcaacgcgg
     6541 cggctctcgg ccctcgtgga gctgacgccg cccccgtcgg gagtcagcaa caccttccat
     6601 ggccgcgacc tctttgccgc cgccgcggcc cgcctgtgct ccgggtcccc gctcaagcgg
     6661 ctcggggagc cagcggcgga tcccgtcctg cttccgctgc ccaggctttc cgtcgatcgc
     6721 gcgagcatcg gtggagagat catcgccatc gaccgcttcg gcaacgccat taccagcatc
     6781 ggcgccattc gcacggctga ggagggcatt gcgctgaccc cttggctacc ggggtgcgcc
     6841 gaagcgcgcc tcagcgggat cgggttccac gtaacactct ccacggggga gagcgtccca
     6901 ctggcgcgaa cgttcgggga cgttccgaaa ggcgatgcgc tcacgtatgt cggaagcgat
     6961 ggcctcatgg agatcgccgt caacggcggc cgcgccgccg atgtcctggg catctcgatc
     7021 gcagatcccg tagagctcca ggcgctctag ccctgctgcg tcaccatgcc ggtcacgagg
     7081 cggatccgct cttccagggt gggcccgacc gcctggtcgg cgatccccag gatgatacgg
     7141 cccgccgatc gggcgcggga gagaacctcg cgggtctcgg acaccaggag atgagtcggg
     7201 ctctgcgact cgaatcgagg cgaggccagg cagccccaga gaaccacccg gtccctccat
     7261 cgctcccagg cacggcggaa gctcaatcgg ctcaggggct cgggggagaa ggactcgacc
     7321 acatccaccc cgctttccgg aaccaggtcg agcagcgcgc tgaggtctcc gtccatgtgg
     7381 ctgccgaggt gtttgccgga gccgtggacc gcctccgcgc tccgctggag cgccggcatg
     7441 cagtagcggg cgaaaagccg cggattggtc atctctccgt ccacgttgtc ggagtgctcg
     7501 accatgaccg ccggcgaggc ggccgccacc gcgaccgatt gggtccgtag ctcctccagt
     7561 cggtccagga gccgggagaa ccgagagggc tcgtcgtaca tctcgaagaa ggttcgctcc
     7621 tcgccgagga agtccagaag cacccgctga aatggcaggc ggtcgatcat cccaatggca
     7681 aggccatcgc cgccgatctc ctcctcccgc tggaggagcg gcgcatactc cggcaggaca
     7741 tcgagccgat cgaggatcca gaagacggcg gcgaagtccg cctcctcggc gatggacctt
     7801 cgggtcagaa acgggagcgt tcccgttgcc aggatctccg gcgtggtggt gtactggatg
     7861 cggcagtctc cgacgggggt cgtgaaggtg aacgtgctca ctccgggacg gttcctcggg
     7921 accaggtcgg taaccagggg gaatcggaga agaggatcgc gctctcgaag aaacgggaca
     7981 ccgtcgaggt ccgcaagcaa ctcgacgcct cgaaggcgat tcaccgttat ctgcacgtaa
     8041 gactggcggc ccacgccgag atcccggtag atgctcatga cggagacacg gtggtccttc
     8101 cggggttccg cgcgcgctcg cgcctccaac cagaggtcca ggcgagtagc ccagggcggc
     8161 cggtccggct ttccaccctc aaggacacac aggattcgct cccgttcggt catcctgggc
     8221 ggtatacccg cgcggcgcga gccctgtcaa tcttggccgc gcggggggct gccacggggg
     8281 agaacggccg cgggaggctg ccacggacga ggaccccgcg actgattcct ccttgccgtc
     8341 gccatcctgc ctccgtacct tttcctgttc gcaaatgaag gctccggccg cgccgggccg
     8401 gcgctggatt gagaatggag gaaccatggc gaaggtatcc acgctcaaga aggcaagggg
     8461 cggctcgccc cgaaaggccg ctgcggccaa aaccgcaaag ccgaagaccc ggagagcggc
     8521 ggcattcgcc gctgcccgat tgcgacccgt tgagctcctg atcggcacca tcaagggggg
     8581 gttcgccttg aaggggtcgg atccgggcag aagctggtcg ctgacgggac cctacttcct
     8641 gggctgcgag gtgaacgacc tggtcctgga cccgtgggac cgacggacgc tgctcatggc
     8701 cgccaagacc ggtcacctgg ggccgacggt cttccgatcc aaggaccagg gcaagagctg
     8761 gcaggaggcc agacagcctc ccgctttccc caaggaacct ggtggaaagg cggtagaccg
     8821 cgtctttttc gtcacgcccg ggcatcccag ccagccgggg gtctggtggg cggggaccgt
     8881 gccgcacgcg ctgttccgct ccacggacgg aggggagagc tgggcgccgg tgcagggctt
     8941 caaccagttc gttgccggcc tgaaggagcg aacgcccaac ctcctcgggg agacgccggg
     9001 aggagcgata acccactccc tcctgatcga ccccagggac gcggcccaca tgtatgtcag
     9061 cctttctacg ggcgggttct tcgagagtcg ggacggtggc cagacctgga agcccatgaa
     9121 ccagggaagc atcgccgtgt tcctgccgga cccaccaccg gagtacggcc aggatccgca
     9181 ctgggcggtc ctgtgccccg cggatccgga ccggctgtat cagcagaacc actgcggcgt
     9241 ctatcgcctg gaccggccag gagagaaatg ggatcggatt gggagaaacc ttcccaagga
     9301 ggtcggcgac atcggctttc cgatcgtggt ccaccctcgg gacccggata ccgtgtgggt
     9361 tttcccgatg gacggcacgg acgtctggcc tcggaccagt cccgccggca gacccgccgc
     9421 gttcagaagc cgggacggag gagcgacctg gactcgccag tcccgcggat tccccagcct
     9481 ggggtggttc agcgtgctcc gacaggccat gaagtgcgat gccaacgacc ctctgggtct
     9541 gtaccttggc accacgtcgg gggaggtctg ggtcagccgg gatgaaggcg ccacctggag
     9601 ccgggtagcg gagcatctcc cgcggatcct gtcgatccag gtggccgcgc ggtgagcgta
     9661 ccggtacacg tgtcgaccca cctccactcg tacaccgggg gcctatccac catcgaggcc
     9721 gatggcgcga ccctggcgga gctcctggtg gatctggacc gacgataccc cggccttcgg
     9781 tttcgagtag tggacgagca ggaccgggtt cgccgtcaca tgaacttctt cgtggggggc
     9841 gaaatgaccc gaagcctgga tcaacggatc gagccgaacc aggaggtcca tatcctcggc
     9901 gcgctgagcg ggggatagag cgaggtcatc agatcgagac cggggggccg tgcgccggat
     9961 agacggtgtg gactccccgg tccttgagac gcgcccagga ctgatgggct tcccggtcct
    10021 ccggagtgag caggatctgg tgcggcaggt ccccggtgaa ggcgagcccc tcgtccagaa
    10081 tgagagagac gctgtctggc gagtgaccgg gcgtggagag gatctcgccg tccaggccga
    10141 tcccctttag aaactcccgg ctctcctcgg ggttgaccac cacgttgtct ccgggtcgta
    10201 tcggcagata cccgctgtcc ggcttggcga tgcgctccag gggcttgacg aacgggatct
    10261 gggcttccgt gacgatcagc agggcgccgg cgtccttcac ctcctggacg agcccggcat
    10321 gatcgggatg gaagtgagaa accaggatgt gcccgatatc ctcaatggaa actccgttcc
    10381 gtttcaggac actgcgaaac gtgccaatcg ttccgggcca accgcagtcg aagaggagac
    10441 gctttccacc gggcggctga atgagatagt agtgcgtcga gcgatagccg acgtcgacga
    10501 ggcggacgtt cacgcgcaga ccgtaaagca cggacggcgg tagcgcaagg cggcggcctt
    10561 gacgggaccg gcgggttccc gacagtctgg cacgggggtc catgatggtt gatggccaca
    10621 gcacctcgta cgcatggcag gtccacaccg gtgacatctg cgtcctgccg gtcggcgccg
    10681 tcgagcagca cggagaccat ctcccgctgg tcacggacgg cgtagcggcg gaatacgttg
    10741 cccggacggt cgcggagcac ctcgacgcgg cgctcctgcc ggctcaactc tatgcgacca
    10801 gtctcgagca cacgggcttc cggggctccg tcaccctgag accggaaacc ctcatgcaga
    10861 tcgtccgcga tgtctcggac gccatggaaa ggcagtcctt tcggatcctc gtcctcgtca
    10921 acggccacgg cggcaacttt tccctgggtc ccgtggttcg ggacatcaac cgaatggacc
    10981 gtgccctgaa gatcctgctc gtgaacctgt gggaatactg tgatcccggg atcgcagctt
    11041 ccagcgcgag cctggggatg gacatccatg cgggggaatg ggagcgatcc ctgatgctgg
    11101 ccctggcgcc cgaggactgc ggagccggac cggggaagga catgccggtt cagccgtcgc
    11161 cgaccctctt gcagcgagac ctgaacacgt tcggagtcgg ccacttcaac ccggcaggat
    11221 cggtgggctt cccctcacga gcgacagcgg ccaagggcaa ggccatcgtc gcctccatcc
    11281 gtagaaacct gcttccggcg ctggatgcgc ggatcgagcg ccttcgcgcc aaccgtcgat
    11341 acgccggctc gggaggcatc gccctgcgga ccatggggga gcaggacgtt cccgccggca
    11401 tgggcctgaa gtccgcggcc gggtggaacc agaccgaggc ggactggcgt cgcttcctgt
    11461 ctctcagccc cggtggatgc caggtagccg tccacaacgg cgccgtggtg ggaacggccg
    11521 ccgtgctgcc gttcgggaag cgcctggcgt ggctgagcat gatccttgtg gccccggaga
    11581 tgcgggggca cggggtcggg accctgctgg cgcgctccgc gctggcggcg gcctctggga
    11641 tcccccttgt caggctggat gccaccccgg ccggccagcc gatgtaccag agccttggct
    11701 tcacggtgga gtacccgttg gctcggcttt ttacccgaag cgcccaccgg gcgatcgagg
    11761 caacccgccc ggcccggcgg gtcgcggggc gtgacctggc cgctatccgc cggctcgatg
    11821 caacggtatt cggggcagac cgcggtccgc tcctggagtc cctcctggga gactacccgg
    11881 agctggcgct gcagatccgc cgggacgggc gggtggtcgg ctactgcatg ggccggcacg
    11941 gcagcgagtg cacccaggtg ggacccgttg tcgccgagag cgagcaggat gcggaggcgc
    12001 tggtgcacgc ggctcttcgg gtcctggccg gccgcgcggt catcattgac gtcccaacgc
    12061 ggcaccagga tttctcggag cacttgtacg gtcttggatt cgggctggag cggccctttg
    12121 ccaggatgag ccgaggaaag ggcagactcc ccgctcgaat cgagcggcag tacgccatcg
    12181 tcggcccgga gttcggttga gcagccgcgg atctatctcc ctgcggccaa cgcgttcgac
    12241 acggcttggg agaaggcctt ggtcgtgagg ctggcgccgg aaacggcgtc gaccggcagg
    12301 gattgctggg aaacgatctg ctgggcgatc cgtcgggtca cgtcgttcgc ggacttgtcc
    12361 gtctcggtga cgccggtgat cttgtggtcc gccacggtga cgtcgacggc aaagcggaac
    12421 cgtcctcgcg tgaactcccc atgatgcgtg ccgtcggaag cgctcgaaac gtcgagcggc
    12481 gtaacgctca tgtggcggac gtagctcatg ccgttgaggg cggcgatcac gccgatagcg
    12541 attaccacta cgatcgctcc caccacgatg aggacgatcc tgcctctccg cttcattggc
    12601 tcctccttgc aggttcgatg cgccgggatc tgagtgcaaa ccgtgtcatg gcggcgacgc
    12661 cggcgtcaag acgggacagg tcgtgcacgg gtggtgcaaa tgaacgcttt ggagagtttt
    12721 ggaatcgaac cgaagcaccg tgcgactggc tggaccgcct cggatcgagc agcgaaaagc
    12781 gcaccggacc ggcaaccgtc tatggcgtta tttgacggtc cctcatccgg tgctctacat
    12841 tagttgcaat ctcagaggct gtgacgcgtg ggacgctaca ttctcggtag aattgccgga
    12901 ctgctcttca cgttcttcat ggtctccgtg atgaccttct ccctcatgca cgcggtaccc
    12961 ggcgggccgt tcgacgagaa caagcagccc ctcccgccgg cagccaaggc gaacatcctc
    13021 cgcaagtacg gcctcgaccg ccccatctat gagcagtacg cgcgctacat gtgggcggcg
    13081 ctccacttcg acttcggcat ccccttccaa agcccgacgg agaccgtaac caagctgatc
    13141 gcgcgggttt ggcccgccac gctccaactg gccggcctgg tgatcctctt gtcctacagc
    13201 ctgggcatgc tcttcggcat cctggccgcg atcaaccaga acacgtggat cgaccgcggc
    13261 gtaaccaccc tggctacgct gggattcacg gtcccgaact tcgtcatcgg gatctggctc
    13321 atcctcatcg tcagcgtgcg cctgcactgg ctgccgaccg ggggctgggg gagtcccaag
    13381 cagttcatca tgccggccct ggtatatgcg caggcgccga tggccctggt ggctcgctat
    13441 acgcgggtga acatgctgga ggccatgcgc gccgactacg tccgcacggc gcgggccaaa
    13501 gggatgagcg agcggaccat catcctgcgt cacgtcctga agaacgcgct tattcccctg
    13561 atcacggtga tcggccccca gatcccgaac atcgcgacgg gctccatctt catcgaggcg
    13621 gtgttccgca tcccggggct gggccggtac ttcatctcga gcacctacaa ccgggactac
    13681 cccatgatca tggccaccat gctcctggcg gctgccctct ggggggtcac gtatctgatc
    13741 agcgacatcc tgtacaccgt catcgatccg cgggtccgac tcacgggacg gagagacgca
    13801 tgagcacgcc ggccgtttcc gccgcgcccg cccggcccgc ggtccgccgc accctgtggc
    13861 gggacgccac ccgcagcttc tcccgaaacc ggctggccat gatcggcttc gccgttgtcg
    13921 cgctgttcgt cctggttgcc atcttcgcga acttcgtggc gccgcagccc tactacaggg
    13981 caaagctctc ggagaacctc cagttcccca gcatccgaca ccttttgggg acggacgccg
    14041 taggccggga cctcttgagc cggatcatct acggtattcg aacatccctg ttcgtcgggt
    14101 tctccgtcca gatcgtcgcg ttcgccattg gggtcacgct gggcgctctc gccggactgc
    14161 gcggagggtg ggtggacttc gtcgtcacac ggatcctgga gatcatgacg gcgttccctg
    14221 gattcctctt cgccattttc ctgatgggca tcctgggcaa cggcgtgctc aacgtgatcc
    14281 tggccatcag cgtaaccagc tggatcgatc cctgccggct cacccgcgga cagcttatcg
    14341 cgctgcggga gcgggagttc gtcgtcgccg cgcactccta tggctccagc gactggcgga
    14401 tcgccatccg ccacctgctg ccccacgcgc tgccgccact ggtcatcatg ctggccctgg
    14461 ggatccccaa cgccatcttc gcggaggccg gcctcagttt cctgggggtc ggtatcaacg
    14521 atccgatccc cagcctcggc aagatggtaa gcgacagcat ggggtacatc agcgtgtact
    14581 ggtacatggg tgtgtttcca accctgacca tcgcgctggc gatgctgagc ttcaactatg
    14641 tcggagacgg ccttcgggac gccctggacc cgacgatgta caaccggtag gcgatgcgag
    14701 gtttcgcctg cggcagtgcg cgaaggagag gaggtagaag gcgcggctgg ggaaagcagc
    14761 cccccagggc cccgggaatc cttccgtaga ccggtccacc ctgggctacc ctcggggagc
    14821 ccagggtgga tccatcttcg gatcgtcttc atcccatgac gaatggagga gttcatgcag
    14881 acacgcagaa gaggagtctt ggctgcactg ccggtgacgg ccgtgctgtt cctggtgatg
    14941 gtgatcgccg cgccggccgc gatggcgcag gtgaagaacg cctggggcgt gacgctgccg
    15001 gcggacgcgg cgccgctcaa ccagcagttc ctgcgggtgc tcgggctgga aggcaccaca
    15061 atggatttcg cggtgagcgt ctacaagcgc cctgccaata tcgacatgct gagcaccccc
    15121 atggtgcaga tcaacaagaa cttcgaggtc gtgcccgccg gcgcgctgag ctacgcggcc
    15181 tccgcggatg gcaagacctg gacgttcaag atggacccca agctgacgtg gagcgacggc
    15241 aacccggtga cggcggatga cgtcgtgttc acgttccagt atcaggccga tcccaagcac
    15301 gcctgggact tcgcctggtt ctggagcgat atcgtgaact gggatgacgc ggtggcgggc
    15361 aaggtgccca ccagcgccat cggagtcaag aaagtggaca cctatacggt gcagttcacc
    15421 accagcgtgc cctctccgta cttcccctcc aaggcgctgt acatccgacc cctgagcagg
    15481 gtggccttcc agaagtacgg cgagtactac gacaatgatc cgaagacctc ggtatcctcc
    15541 agcccctgga tccttcagga gtggacgaaa ggggttcgga tcgtcctggc gccgaacaag
    15601 aagtacacgg gccgcgagaa gccgtatctc cagtacctga tctacgagat cggagacctc
    15661 gggacggagt tcaacgcgta ccagaacggg gagatcgacc tggcgttcaa cttctctccc
    15721 gccaacatca cgaccatcaa caacgatccg cagctcagca aggagtacca cccggggtac
    15781 ggtgacttcc gcacctatta cctgggcttc gacaccacca ccaagccgtt cagcgacctg
    15841 agggtgcgcc aggccttcgc caaggcgatc gaccgggaca cgctcatcaa gaacgtggtg
    15901 ggacgtcagg gcatggcggc ctactctttc ctgatgcccg gctttccgga cgccaactcc
    15961 aaggcgctga gccaggagaa cgtaaacaaa tatgacgttg ccgcggctca gaagctgctg
    16021 gcggatgccg gctatccggg tggcaaaggg ttcccgtccc tggagatgtg gctgcgcacg
    16081 gagaactcgt tcaacgagtc catcgccgaa gcagtcgcct ccatgatcaa ggacaacctc
    16141 ggcatcgacg tccaggtatc ccagaaggag cagaagctct tcatggacac gctgaacgcc
    16201 cacaagctgc ccttctacta cctctcctat gggttcgact acctggaccc ctccaacatg
    16261 ctgggcatct gggtgaccgg cgggcgccat acctggtcga acccccagtt cgacagcctg
    16321 atcaagcagg cgagctctct ctccggcaac ccggccaaac gcgaccagga gttcaaggac
    16381 gccgaaaaga tcctcgtgga cgacgtcggc ggggtgttcc tctaccacgt cacgccggga
    16441 acggtctaca agccttacat gaagggctcg gagctgcttc cggacaagac cggtgtcgct
    16501 gcctggcact ggccgtatga ggaagatgcc agcacgtcgt tgcccaccat ttatgtgacg
    16561 aacgacgtcc cttcggatcg acatatgtag tcccgttcgc ccgtaccgcg cgggctctcc
    16621 ttccggaggg cccgcgctgc cgggcagcat cgcaacccca atggaccagg cgctactcga
    16681 cgtgcaaggg ctggagactc gtttctggac gcgcgaaggc gcggtacatg cggtgaacgg
    16741 aatctccctg cgcctggaaa acggtgagac ggtcggcatc gtcggagaaa gcggtagcgg
    16801 caagagcgtg accatgctca gcgtcctgcg gctcctgccg acgcctcccg ccgagatcgc
    16861 ggccggttcc gtcctgttcc agggtcggga tctgctgacc atgagcgacg cggacatccg
    16921 cgccgtccgc ggcaggcaga tcagcatggt gttccaggat ccgatgacct ccttcaacgc
    16981 cgtgctcacc atcggtcgcc agatcaccga gccgctccag gtccacatgg gcatgtcccg
    17041 ggcccaggcg tgggtccggg cggccgagct cctggacctc gtgggcatcc cgaaggcaaa
    17101 ggaccggctg cacgactttc cgcaccagtt ctcgggcggg atgcgccagc gcgccatgat
    17161 cgccatggcc cttgcctgcc atcccaacgt actgatcgcc gatgagccca ccacggccct
    17221 ggacgtcacg atccaggctc agatcgtgga gctggtcaaa cgcctgcgcg acgagatggg
    17281 gatggccatg gtgtggatca cccatgacct cggcatcatc gcgggcctgg cccagcgggt
    17341 cgccgtgatg tacggcggct acttcatcga ggagtccccg gtgcgggacc tgtacgcgga
    17401 tccccggcac ccctacacca tcgggctcct gggcagtctt ccgcgcgtgg acgtgggccg
    17461 gcggcggagc ctgacctcga tcgacggaag accgccggtg ctcttcgaga aacctcggta
    17521 ctgcccgttc gtctcccgct gccgatacgc gattgcgcgc tgcagccagg agaaccccgc
    17581 gctggaagag gtggccccgg gtcatcgggt ggcgtgctgg gtcaagccca ggctggaaaa
    17641 gcgcgacgga gacggaaccg tggggaaggc ctgatcgtgg cggtggacgc gcttctacgg
    17701 gtagagaacc tcgtcaagca tttccccatc atggccggaa cgatcgtccg tcggcaggtt
    17761 ggccagattc atgcggtaga cggcgtctcc ttcgatctcc atcggggaga gaccctcggg
    17821 ctggtcgggg agtcgggctg cggcaagtcc actaccggtc ggaccatcct tcagctctac
    17881 cggcccactt ccggatcggt ctacctggac ggggtggacc tggcacggct caatcgcaag
    17941 gagctgcgcc cggttcgtcg caccatgcag atgatcttcc aagatcccta cgcgagcctg
    18001 aacccgcggc ttacggtcgg ggagatcgtc ggagatcccc tggtggtcta tcgggaggga
    18061 agccgaaggc agatccagga ggaggttcga acgctcctgg agctggtccg gctcgatccc
    18121 tcgtacgcca accgttatcc ccacgagttc tccggcgggc agcgacagcg catcggcatc
    18181 gctcgggccc tggccctgcg tccgaagctc gtcgtctgcg acgagcccat ttccgcgctg
    18241 gatgtctcca tccaggccca gatcatcaac ctgctggtgg agctccagaa gcaactccac
    18301 ctcacgtatc tcttcatcgc ccacgacctc tcgatggtgc gccacgtgtg cgaccgggtg
    18361 gccgtgatgt acctgggcgt catcatggag cttgccgaca gcgtcggcct ctatcaggat
    18421 cccctgcacc cgtacaccaa ggcgctcctc tcctccgtac ccattccgga tccggtggtc
    18481 gaggagaagc gaaaacgggt catcctgagc ggagagatcc cttctcccgc tcatccgcca
    18541 tcggggtgcc ggttccgcac ccggtgtccc atcgcccagc cggtgtgcgg tgagacgacc
    18601 ccaccgcttc gggagctgcg gcccggccac ttcgccgcct gccacttcgc atagatttcg
    18661 gttaccgcct gcggggtgtc tgcttgtatt accctgcgag gaggtcctgc gtgaaaaccc
    18721 tgttcaaggc tatcgttccc ttgtccttcg gtttcatcct caccctgagt cagtgctccg
    18781 tcctcaagct ggatgtcccg ggcgctgccg gaacctggag cggaacctgg aacaacacga
    18841 cgttcaatag cactggatcg gccacgatgg tgctcacggt ggatagcgcc gcaaaaaccc
    18901 tttccacggt cacgaccctc ggcggcaacg ttttcgggca gggcaacaat cccccggctc
    18961 agaccgtcac cggcacctac accgacagcc tgatcaccct gagctccaca acggcgttgt
    19021 tcggcgccgt gaccctgacc atcgacaaca tgggcaagat cagtggcacc gcgcatccca
    19081 gcggattcga ctccgtgagc gtcagcggca cggcgagccc cacgaagctg gacatgacgt
    19141 acacgatcta ccagcccgcg ggcaccgtct atgccacggg tactctgacc ttcaccaagc
    19201 agtaggatca tccaccgcgg gtccgttttg cggacccgcg cttcttcgcg gcggcggcgc
    19261 gcttcttccc ggccgagccc tgcttcgcgg cagccgcgcg cttcttcccg gcagggcggg
    19321 gtgtcccacg catgcggacg gcggtgggat gccgtggctc gtagagaccg agaagcttgc
    19381 cgtccggtag ccggatccgg gctagcatgc cccatctctg atccgtgatc gggccggcca
    19441 tccgagctcc tcgctcctcc agtctccgca cggccgcggt gacgtcggag cacatcaggt
    19501 acagctcgtg gcgggccgcc ccatccgtgg ggtgaacggc gatctccccc ggtggtgcgg
    19561 caaagatcag ccagccatca ccggcgtcta cggacgccag ccccaacacg tctcgaaaaa
    19621 aggcacggac ctcttcgggc ttccggctgt agaggattgt gtgaactcca ccgacgattc
    19681 cgcccgtgac tgccgggcga gcccctgtcc tcgttgaccg cggcttcatg tgcgctcctt
    19741 gcgcctactg tacggaaccg cgccttccga tggaagatgg ggcattcgca agggatcccg
    19801 gaggattgac cgatgcccga ggccatcgtg cgggtccgcg tggatgccgc cgaggatctt
    19861 ggagagttgc ctcgaatctg gaactacatc ggctacgacg agtgcaacta cacgtacgtc
    19921 ccggaagggc gagccctctt ggctctcctg ggaaggctcg ggagacccgt ctatgtgcgg
    19981 acccaccacc tcttctgctc gggcaactgc cacggtaccc ccaagtgggg atccaccaac
    20041 gtctaccggg aagacgagag aggccagccc gtatacgact ggacgttcat cgatctcgtc
    20101 ttcgatacga tcctttccag cgggtgtcgg cccttcgtgg agctgggctt catgccgcgg
    20161 gatctggccg atccgacacg ctacgatgcc gcctcggggg actggaccgc cagtgcctac
    20221 cgccgcgccg gctgggcatg ccctcccaag gactacggcc gctggcatga gctggtcctg
    20281 aggacggtac gacactgcct ggagcgctat ggcgagcccg aggtcctcca gtggtactgg
    20341 gagctttgga acgagccgga tattccgtac tggcgaggga cccgggagga gttcgagaag
    20401 ctctacgact ataccgctgc agccgtgaag agcgcccatc cccatgcacg tgtgggcgga
    20461 ccggcggtga ccaaccctgc ccctgggaag cccgggtcgg agtttctgtc ctccttcctg
    20521 tcccacacct ccgtgggcac gaaccactgc acgggaggcc gggggacgcc cctggacttc
    20581 gtcaccttcc acgccaaagg gggcggctac tccgcgaacg ccagccgccg caaggagagc
    20641 cctccctccg tacggacgct cgtggagtcg gtcgtgtccg gccaccagat catagaccgg
    20701 tttccggatt accggggcct ggagtgcgtg atctcagaat gtgacccgga cgggtgggct
    20761 gccggcggtg cctgggacaa cgtgaacctg gacttccgaa acacggagta ctacgcctcg
    20821 tacgttgcct gctcgttcga ccgggtcctc aagcacggtc tggcgaaagg atggaaggtc
    20881 aaggcgctga gctgggcctt cctcttcgtc ggcgagcgct gcttcgaagg gacgcggacc
    20941 ttcaccaccc aggggatcgg caaggcgatc ctcaacctgt tccgcatcta tgcgcgcttg
    21001 ggggggcgga tggtgaagct ggagagcagc gcctcccagg agccgcgttg cgatccggac
    21061 ggcgaagctc cggtcgtgga cggtgttgcg tgcctgggat ccgggcggct gacggttctc
    21121 ctgtatagcc accatgtcga ctgggatcgg aacggttcca cagccgtctc cctggaggcg
    21181 gtcaacctgc cgtttggcac ccggcgttgc agggtgcggc atttcagagt cgatgccgcc
    21241 cacagcaacg cccacgcgga atgggtccgc caggggcagc cgctgtatcc ttctaccgcc
    21301 caacgaagag ccatcaagcg gcgtgaaggc ctggagacgc tggaaccgcc tcgtgccgta
    21361 gcccttctgg gtggaacgct aaagctccgg ttcacgatgc ccgtaagagc ggtctctctt
    21421 ctggtcataa cgcctgccgt gcgaaggggg gaatcctgag atgcgttttg gagtttgcgg
    21481 atctacggtc gttcccggaa gctccggcgt ggagatcatc gagaaagccg gcaacgccgg
    21541 ctacgattat atggagcttt cgctcagcca catcagcgcc ctcaccgagg agaccttcgg
    21601 agccctccgc gcgcgtgtga cggattcggg agtcccgtgt gaagcctgca acaacttctt
    21661 tcctccgtcg gtccggctca cggggcctga ggtgcgctgg agcgccgtcc aggactacac
    21721 gcggctcgcg ctgggccggg ccttggcgtt gggggcacga gtcgtagtct tcgggagctc
    21781 gggggccaag aatgtccccc cgggcttccc gaaggagaaa gcgctggacc agatcgtgtc
    21841 cgcgcttcgg ttcgtagccg aagaggcggg tcctcgcggc atcaccatcg cgatcgagcc
    21901 gctgaaccgt aaggagagca atatcgtcaa cgaccttacg gaagcactgg ccctcatgaa
    21961 cacggtggat cggcccgaga ttcgggtcct cgtggattat taccattttg ccctggaggg
    22021 cgacacaccg gaggcccttc tgcgggccgg cccctacctc aggcacgcac acgtggcccg
    22081 cgtggagggc agggccttcc cccaggagcc cgacgcgggc ctgcgatcgt tcttccggat
    22141 gctctccggc gttggctacc aggcgcgggt cagcgtggaa ggggcaacct cggatttcga
    22201 ccgggatgga ccgcaatccc tgcggacgct ccgcgcgatg gcctccgctt gagcggtctt
    22261 caggttaaga aggtttaaca cacccgcaaa cggttctaaa cggtgcctcc gtaccttgtg
    22321 gacaggagga cacttcgtat gaagagaaca accaagacgg tggccattgc cgcgggcgtg
    22381 ctcgtcgtcg gcctggccgc tggaatgctt ctggctcaag gacgttccga gagcctcgcg
    22441 acgggccgga cgccgaccca gaccgcttcc gtgcaggcgc tgccggtggc cgacccgtca
    22501 tccctgcaga gcctgcagct ctccttccga gaggtggctc agcggatcat cccctcggtc
    22561 gtggagatca acgtcacgga gatggtcaat cagccgaccc aggatttcag ttttccgttc
    22621 cccttcccgt tcggacagca acagccgcag ggaggttcgc agcgcgtgcc ggtttccgcg
    22681 ctgggatcgg gcatcatcgt ccagcatacc ggcaatcgtt acttcgttct cacgaacaac
    22741 cacgtggtgg atagcgccac ccaggtgagc gtgcggctca acgaccagcg gacgtataag
    22801 gccacggtgc ttggcaagga cccgctgaag gatctggcga tggtagcgtt cgacacctcg
    22861 gactccattc ccgtcgccgc cctcggtgac tccaacagcc tccaggtcgg agacatcgtc
    22921 ctggcggtcg ggaacccgtt cggctacacg gacaccgtga ccatgggtat cgtcagcgcc
    22981 ctgggcagac gcggacctcc gggtcaacag ccctccacgt acaccagcta catccagacc
    23041 gacgctgcca tcaaccaggg caactcgggc ggcgccctgg tgaacgtaga tggccaggta
    23101 gtggggatca acacctggat cgccgcgccg aacggcggga acatcggcct gggcttcgcg
    23161 attccgatcg acaccgccaa gaccgatgtc cagcaactgg cgaccaaggg tcaggtgcag
    23221 tacggctgga tgggcgtcga gatcagcgac atccaggact ccgccaccta ccccgggtac
    23281 gccaccgacc tgaagctcca gggcgtcaac ggggccatgg tgatggacgt ctacaagggc
    23341 tctcccgcgg ccgagggcgg tgtgctgccg ggagactacg tgacggcggt ggactccacc
    23401 ccgatccaga acgcggatca gctcacccag gtggtcggga acctcatcgc ggggcggacc
    23461 tacaccttct cggttctccg ggacggaacc aggaccgatc tccgcgtgaa aatcg
//
LOCUS       DASRZS010000130        10620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4303636, whole genome shotgun sequence.
ACCESSION   DASRZS010000130 DASRZS010000000
VERSION     DASRZS010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10620
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4303636"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..752
                     /locus_tag="VFH83_03200"
     CDS             135..752
                     /locus_tag="VFH83_03200"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HET6485399.1"
                     /translation="MFLALGAPSWAQAKATAASGGSPAAPASPAASAKPGSSQQINES
                     DLTLPDSGAPAAGGAAAPASSGGVSTWDFVRMLLILAAVVGVIYLLFWLLRRGAGRRI
                     QENSLIRVLGSRNLAGNRSLHLVEVGKSVFLVGAADGGVDLISEIKDQESLDAVHLSA
                     AEERPSARRTFQQTLGEIFRPAKSSISLTESVGLLRRQRDRLRKL"
     gene            749..1591
                     /gene="fliP"
                     /locus_tag="VFH83_03205"
     CDS             749..1591
                     /gene="fliP"
                     /locus_tag="VFH83_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612470.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HET6485400.1"
                     /translation="MRRLLSILLILTTLSGVAAPAAYAQAAAAGGSAAPAGRAASGGA
                     AAGTAGVASLPIPFVDLNVRTARNNQEVVLSVQLLLLLTVLAIAPSILILTTCFLRIA
                     IVFDFIRRALSLQSSPPNQVMMGLALFLTLFIMWPTFNDIYQNSLKPFGDGKIGLDQM
                     FSTAEKPLRIFMFRQMGNDVGSIQLFMSMRGLDKPRTLADVPTYVLIPAFVIHEMTTA
                     FKIGILLYIPFIIIDLVVASTLMSMGMIMLPPVMISTPFKLILFVLVDGWGLLTMQLV
                     RSFR"
     gene            1588..1851
                     /gene="fliQ"
                     /locus_tag="VFH83_03210"
     CDS             1588..1851
                     /gene="fliQ"
                     /locus_tag="VFH83_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014073988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HET6485401.1"
                     /translation="MSVGMAIQIVRDGILETMVVAGPILLTALGVGLIIAILQATTSI
                     QEQTLSFVPKIAAILVGLLIFGGFLLNSLVEYTRRLFAMIPNL"
     gene            1848..2639
                     /gene="fliR"
                     /locus_tag="VFH83_03215"
     CDS             1848..2639
                     /gene="fliR"
                     /locus_tag="VFH83_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HET6485402.1"
                     /translation="MIEALLSNIRVFLLVFFRIIAMVELTPLLSSASIPQVAKIGFAF
                     FAAASAFPGVLAAGYPLPASTLDYAFLVLGEVAIGLLIGFLLLLIFSCFQLAGQFFSL
                     QMGFSASEVFDPLSQVEIPLMGEFLNLVAMLVFITMSGMGQFLLVGVQRSFQYLRAVD
                     LVTHRDQVIQLLVGGMGGLFQSALTISFPILGTLLLVSIVLGLMAKAAPQMDVLSMGF
                     PLSIAVSFILLFATLPFLMTAFEGIIEGSFHTLGQFITGVGALRK"
     gene            2636..3820
                     /gene="flhB"
                     /locus_tag="VFH83_03220"
     CDS             2636..3820
                     /gene="flhB"
                     /locus_tag="VFH83_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HET6485403.1"
                     /translation="MTRTFLLAAAPDRSLLLERWVPALDSPYDVSIQWFAAEDEGRTE
                     EPSEHKLRKAREEGKVARSAELSSALVLLAGVGALGILANNILGSSVQMFSFYLSRAA
                     DPEALNATLVHSFLGWLVRIGLPLLVVVFVVAIVGNVIQVGFLFTVKPITPDFSRIVP
                     RFSRWLQRSFASGEALFNFAKAVGKLAVIAGIAYANIRGELPRLAMLASASFVGGIAV
                     ISQIAFRILVEIAIVFLVFSAVDYWFQRRQHLESLKMSKQEVKEERKMYEGDPLTRSR
                     LRERMREIMRRNMLKAVPKADVIITNPTHYAVALEYNRALMAAPTVVAKGADEVARKI
                     REIATENGVPIVENKPLAQALYREVEIGDAIPEKFYEAMSTILAQVYKLTGRSAGFEA
                     AV"
     gene            3854..5950
                     /gene="flhA"
                     /locus_tag="VFH83_03225"
     CDS             3854..5950
                     /gene="flhA"
                     /locus_tag="VFH83_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HET6485404.1"
                     /translation="MADARAVVGNLWQQRSDVLVAVGVVVVIMMLLIPLPAVLLDVLL
                     AANLVLSLLIILTVLYTRHALEFSVFPTLLLIATVFGLALNVSTTRLILSRGSQFNGH
                     IVRAFATFVVGTRGVEGYVIGLIIFVIIIAVQFIVITKGATRVAEVAARFTLDALPGK
                     QMAIEAEYNSGLITEEEATRKKNDLQREADFYGSMDGASKFVSGNVRVGILITVIQMV
                     GGLIVGMTLHGEPLNLALDTYISLSIGDGLVTQLPALLISTATGIIVTRAISDSSFGK
                     DITKQFSSQSRPYLIAAAFLVLLSFLPGFPWYVLIPMGALIGVLGFSLTRRQIAEEEK
                     TKLKEVADREKAAPAEISPVVPLDPLSLEIGYGLIPLVDKEKGAELLDRITRIRRESA
                     LELGLVVPRIRIIDNMRLEPSEYSFKIRGVEVGRGKIKMGHYLAIQPGKVAEEIRGEP
                     THDPAFGLPAVWIVEEEREHAERLGYTVVDPPSIIATHLTEIIKRHAADILGRQEVQS
                     ILDTLKNDYPAVVEEVRKALGVGEIQKVLQGLLQERVSVRNMVAILETLADYGPVTKD
                     IGFLIEKVRQSLGRQICLQYCDDKKQLRVLTLEPALEKKIIDSRLETNRGIVSGLDPA
                     THRRFVTAMTNAVKTVQEAGYHEVVLCSEAARPLVKSATAREIPHLAVLSSLEISADV
                     SVESLGEVRIETDGKG"
     gene            5969..7174
                     /gene="flhF"
                     /locus_tag="VFH83_03230"
     CDS             5969..7174
                     /gene="flhF"
                     /locus_tag="VFH83_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HET6485405.1"
                     /translation="MQYFTIQARSNREAIEKMKSTYGEDARILTHRAVRMGGFLGLFA
                     AEGVEVTGYLSDEGVRRKKVEVEEEKKRILESVKKEQTLQLLLREIQGLRESIQARPP
                     APPEPPAAEELTSLKQLEDLLVANEFAPDFASGLVTRARRELSLETLENFALLQNAAV
                     EWIGECIAIDPPLSGRSPRVVVIVGPTGVGKTTTIAKLAAIYGIGDGRTPARKVRIIT
                     IDNYRIAAKQQIETYAEIMRIPVNLVESAEDLRKNLAVYQDTDLVLVDTIGRSPRDLS
                     KLAEMKEILATCGGRAAVYLALAATTKGSDTEEILRQFEPFGYRSVILTKLDETMRIG
                     NIVSCLTRHRKSIAYLCDGQGVPQDLEEASVVRLLMNMDGFRIDRDTMERRFARPRAD
                     TGDARGRGA"
     gene            7180..8133
                     /locus_tag="VFH83_03235"
     CDS             7180..8133
                     /locus_tag="VFH83_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HET6485406.1"
                     /translation="MADQAEKLRQLVSSSAAGSQGAGTGPEAGSAPEAPTRVKEKSSP
                     ARPPEGRAATRIIAISSGKGGVGKTNIAINLALAYAKAGKKVIVMDADLGLANVNVVL
                     GVIPRYNLYHLIRKQKTLSEILVDTSYGIQIIAGASGFSKIANLSEEERRDFIGELAA
                     LSNADILIIDCAAGVSNNVISFIAAADDALIVTTPEPTAITDAYGIIKIIATEIENLD
                     LGLKLVVNRVKSVVEGKRVAERVINIAGQFLNLKVDYLGFVFDDPVVHTSVVRQKPFF
                     LLEPRSRASVCIRHLVGRLENYDYRDGKGIPVFLKKLFGGK"
     gene            8222..9004
                     /locus_tag="VFH83_03240"
     CDS             8222..9004
                     /locus_tag="VFH83_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485407.1"
                     /translation="MELFREYGKTAGIFAGAAFLLSLLVGALSRNPFGVVLLRALLFG
                     LLFALLGGGYRYLYLRFLSQPSGSGAEKAAAASGSAVDIVLPEEPATYEPSASGEDAE
                     AVEPEEVAEAEASGEEARPAQSVAGAGPTEDSGDLLDAETEGESEDELLPVMEEADSG
                     SAASGDGQSGLDELPDLMDLDAPARPGGRGRAGEQRGAEPRPGEPDAAGSRPAAPGPG
                     RRRATPSPGTTPEEAVRNAVSEEDPASLARAIRTVLKRDGKG"
     gene            9012..9800
                     /gene="whiG"
                     /locus_tag="VFH83_03245"
     CDS             9012..9800
                     /gene="whiG"
                     /locus_tag="VFH83_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HET6485408.1"
                     /translation="MAESVWDGKSEEDLWRVYRRTRDQKVRDYFVRQYAPLVKYVAGK
                     IALGMPQNVEFDDLVGFGIFGLFDAIDKFDPSKHVKFKTYAVTRIRGAIFDELRTIDW
                     VPRSVRQKAREVEETVRRLESSLGRAATDEEIASGMEMEVKEFQQMMLRISGTSMLSL
                     HDIWYTGEDNDKISIAESIESPGSLNPDTIVEKEEIKRVIVEAIKELPDKEKKVLVLY
                     YYEDLTLKEIGEVLEVTESRVSQLHTKAVMRLRAKLTSFKRGIV"
     gene            9951..>10620
                     /locus_tag="VFH83_03250"
     CDS             9951..>10620
                     /locus_tag="VFH83_03250"
                     /inference="COORDINATES: protein motif:HMM:NF026154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HET6485409.1"
                     /translation="MATLIGLEELRDLMRKQSEEDRKNRTITVEGPSVEEALKRASIE
                     LGVPVRRLEFEVAIRGSDGMLGMGRKPWKLSVYEKSQMVKTEQESQADALREEELSAA
                     EAQRPKDRPGEVFVRIQADGVYLKVTRPQGRGPKATELMALERLSLRGVSRFDEGLVS
                     RVVKHADGEYVHVADVQYNPNNDSTVSMDITDGSMKAVVVLSEPGPGGADISAEYLRS
                     VLQSN"
BASE COUNT         1810 a         3417 c         3545 g         1848 t
ORIGIN      
        1 gatgaatgat ctgacatagc gtatccgttt cagattttac tgggaggggg aatctgaaaa
       61 cgcaaggcaa ggcgcgcgct catgcgcgcg caggcagcac gcattggcgt gcgttcgcgg
      121 cggcggcggc cgcggtgttc ctggctctgg gcgctccgtc gtgggcgcag gccaaagcta
      181 cggccgcatc ggggggctct ccggcggcgc ccgcctcacc ggcggcgtcc gccaaaccgg
      241 gctccagtca gcagatcaac gaaagcgacc tgaccctgcc ggactccggc gctcctgccg
      301 ccggcggagc cgccgccccg gcctccagcg gcggcgtctc cacctgggat ttcgtgcgga
      361 tgctcctcat cctggccgcg gtcgtcggcg tgatctacct gctcttctgg ctgctgcgtc
      421 gcggtgcggg tcgtcgcatc caggagaaca gcctcattcg ggtgttgggc tcccgaaacc
      481 tggcgggcaa tcggtccctt cacctggtgg aggtgggcaa gagcgtcttc ctggtgggcg
      541 ccgcggacgg cggcgtggat ctcatctccg agatcaagga ccaggagtcc ctggacgcgg
      601 tccacctcag cgcggctgag gagcgacctt cggcgcgccg caccttccag cagaccctcg
      661 gagagatctt ccggcccgcg aagagctcga tctccctcac ggagagcgtc ggcctgcttc
      721 gccggcagcg ggatcggttg aggaagctat gaggcggctc ctctccatcc tcctgatcct
      781 gacgacgctg agcggagtgg cggcacccgc ggcgtacgcg caggccgcgg ccgccggcgg
      841 atcggcggca cccgcgggcc gcgcggcctc cggcggagcc gcagccggga ccgccggcgt
      901 cgcgagcctc cccatcccct tcgtggatct caacgtgcgc acggcgcgga acaaccagga
      961 ggtggtcctc tcggtccagc tcctgctgct cctgacggtt ctggcgatcg ccccctccat
     1021 cctgatcctg actacctgct tccttcggat cgcgatcgtg ttcgacttca tccgcagggc
     1081 gctctcgctc cagtcctctc ctcccaacca ggtcatgatg gggctggccc tcttcctcac
     1141 cctgttcatc atgtggccga ccttcaacga catctaccag aactcgctga aacccttcgg
     1201 ggacgggaag atcgggctgg accagatgtt ctccaccgcg gagaagcccc tgcggatctt
     1261 catgttcaga cagatgggca acgacgtggg gtcgatccag ctcttcatga gcatgcgggg
     1321 cctcgacaag ccccggaccc tggcggacgt ccccacctac gtgctgatcc cggccttcgt
     1381 gatccacgag atgaccacgg cgttcaagat cgggatcctc ttgtacatcc ccttcatcat
     1441 catcgacctg gtggtggcct ccacactcat gtcgatgggt atgatcatgc tgcctccggt
     1501 gatgatctcc acgccgttca agctcatcct cttcgtgctg gtggacgggt gggggcttct
     1561 caccatgcag ctcgtcagga gcttccgatg agcgttggaa tggcaatcca gatcgtgcgg
     1621 gacggcatcc tggagacgat ggtcgtggcc ggccccatcc tgctgacggc gctgggggtc
     1681 gggctcatca tcgccatcct ccaggcgacc acctccattc aggagcagac cctctccttc
     1741 gtgcccaaga tcgcggcgat cctggtaggg ttgctgatct tcggcgggtt cctcctgaac
     1801 tccctggtgg agtatacgcg gcgcctgttc gcgatgatcc ccaacctatg atcgaggccc
     1861 tgctctccaa catccgcgtg tttctcctcg tgttcttccg catcatcgcg atggtggagc
     1921 tcacgcctct gctctcttct gcgtcgatcc cgcaggtcgc gaagatcggc tttgccttct
     1981 tcgcggccgc ctctgcgttc cccggggtcc tggcggccgg ataccctctg cccgccagca
     2041 cgctggacta cgcgttcctg gtccttggtg aggtcgccat cgggcttctg atcgggtttc
     2101 tcctgctcct catcttctcc tgcttccagc ttgccggcca gttcttcagc ctgcagatgg
     2161 ggttcagcgc ctcggaggtc ttcgacccgc tctcccaggt ggaaatcccg ctcatgggcg
     2221 agttcctgaa cctggtggcc atgctcgtgt tcatcacgat gtccggcatg ggccagttcc
     2281 tgctggtggg agtccagcgc tcgttccagt acctgcgggc cgtggacctg gtgacccatc
     2341 gggaccaggt catccagctc ctggtggggg ggatgggcgg gctctttcag agcgccctga
     2401 cgatctcctt ccccattctg gggacgctcc tcctggtctc catcgtgctg gggctgatgg
     2461 caaaggcggc gccccagatg gacgtgttgt ccatgggctt cccgttgtcc atcgcggtct
     2521 ccttcatcct gctcttcgcc acgctcccgt tcctcatgac ggcgttcgag gggatcatcg
     2581 agggaagctt ccataccctc ggccagttca tcaccggcgt gggggcgctt cgaaaatgac
     2641 ccggaccttc cttctggcgg ccgcaccgga tcgatctctc ctcctggaac gctgggtccc
     2701 tgccctggac tccccctacg acgtctccat ccagtggttc gccgcggagg acgagggccg
     2761 gaccgaagag ccgtccgagc acaagttgcg caaggcccgg gaggagggca aggtagcccg
     2821 atccgcggag ctgagctccg cgctggtgct cctggcgggt gtgggtgccc tggggatcct
     2881 ggccaacaac atcctgggct cctcggtcca gatgttcagc ttctacctct ctcgcgccgc
     2941 ggacccggag gctctgaacg ccaccctggt gcactccttt ctgggctggt tggtccgcat
     3001 cgggcttccg ctcctggtgg tcgtgttcgt ggtggccatc gtggggaacg tgatccaggt
     3061 ggggttcctc ttcacggtaa agccgatcac cccggacttc agccgcatcg tgcctcggtt
     3121 cagccgatgg ctccagaggt ccttcgcctc gggggaggcg ctgttcaatt tcgccaaggc
     3181 cgtcggcaag ctcgccgtca tcgccggcat cgcctacgcc aacatccgcg gggagctgcc
     3241 gcggctggcc atgctggcca gcgcctcctt tgtcggcggg atcgcggtga tctcccagat
     3301 cgcgtttcgg atcctcgtgg agatcgccat cgtcttcctc gtcttctccg cggtggacta
     3361 ctggttccag cgccgccagc acctggaatc cctcaagatg tccaaacagg aggtcaagga
     3421 ggagcgcaag atgtacgagg gcgatccgct cacccgcagc cggctccgcg agcggatgcg
     3481 cgagatcatg cggcgcaaca tgctcaaggc cgtgcccaag gcggacgtga tcatcacgaa
     3541 ccccacgcac tatgccgtgg ccctggaata caaccgcgcc ctgatggcgg cgcctaccgt
     3601 ggtggcaaaa ggcgccgacg aggtggcccg gaagatccgg gagatcgcca cggagaacgg
     3661 cgtccccatc gtggagaaca aaccgttggc gcaggcgctc taccgggagg tggagatcgg
     3721 ggacgccatc ccggagaagt tctacgaggc catgtccacg atcctggccc aggtgtacaa
     3781 gctgacgggt cgatcggccg gattcgaggc ggcggtatga agggtcgcgt agcggtgcta
     3841 ggaggtggtt cgaatggctg acgcccgcgc cgtggtcggg aacctctggc agcagcgcag
     3901 cgacgtgctg gtggccgtcg gcgtggtcgt ggtgatcatg atgctgctga tcccgctgcc
     3961 ggccgtcctg ctcgacgtgc tcctggccgc caacctggtc ctgtccctgc tcatcatcct
     4021 cacggtcctc tacacccgcc acgccctgga gttctcggtc ttccccaccc tcctcctgat
     4081 cgcgacggtg ttcggtctgg ccctcaacgt ctccaccacg cgcctcattc tctcccgggg
     4141 gagccagttc aacgggcaca tcgttcgcgc cttcgccacg ttcgtcgtgg gcacccgggg
     4201 cgtggaaggg tacgtgatcg gcctcatcat cttcgtgatc atcatcgccg tgcagttcat
     4261 cgtcatcacc aaaggcgcca cgcgcgtcgc cgaggttgcc gcccggttca ccctggacgc
     4321 cctgcccggc aagcagatgg ccatcgaggc cgagtacaat tcggggctca tcacggagga
     4381 ggaggccacc cgcaagaaga acgacctgca gcgggaggcg gatttctacg ggtccatgga
     4441 cggcgcgtcg aagttcgtat cggggaacgt gcgcgtcggc atcctcatca cggtgatcca
     4501 gatggtcggc gggctgatcg tcgggatgac gctccacggt gagcccctga atctggccct
     4561 ggacacctac atcagcctct ccatcggcga cggtctggtg acgcaactcc ccgccctcct
     4621 gatctccacc gccacgggca tcatcgtcac gcgcgccatc tcggattcct ccttcggcaa
     4681 ggacatcacc aagcagttct ccagccagtc gcggccctac ctcattgccg cggcgttcct
     4741 ggtcctgctc tcgttccttc ccgggttccc ctggtacgtg ctcatcccca tgggggccct
     4801 gatcggcgtc ctgggcttct cgctgacccg gcgccagatc gccgaagagg agaagaccaa
     4861 gctcaaggag gtggcggacc gggagaaggc ggcgccagcg gagatctcac cggtggtgcc
     4921 cctggatccc ctgtccctgg agatcggcta cgggttgatc ccgctggtgg acaaggagaa
     4981 aggcgcggag ctgctggacc ggatcacgcg gatccgccgg gaatccgccc tggagctcgg
     5041 cctggtggtg ccgcggatcc ggatcatcga caacatgcgc ctggagcctt ccgagtactc
     5101 tttcaagatc cggggagtcg aggtgggtcg aggcaagatc aagatgggcc actatctggc
     5161 catccagccc ggcaaggtgg cggaggagat ccgtggagag cccacccatg accccgcgtt
     5221 cggtctgccg gccgtctgga tcgtggagga ggagcgggag cacgcggaac ggctcgggta
     5281 taccgtggtg gatcccccgt cgatcattgc cacgcacctc acggagatca tcaagcggca
     5341 cgcggccgac atcctgggac ggcaggaggt gcagtccatc ctggatacgc tgaagaacga
     5401 ctaccccgcc gtggtggaag aggtccgcaa ggcgctgggg gtcggggaga tccagaaggt
     5461 cctgcagggg ctgctccagg agcgcgtgtc ggttcgaaac atggtcgcca tcctggagac
     5521 cctggccgac tatggcccgg tgaccaagga catcggattc ctcatcgaga aggtgcgcca
     5581 gtcgctggga cgccagatct gcctccagta ctgcgacgac aagaagcagc ttcgggtgct
     5641 gaccctggag ccggcgttgg agaagaagat catcgattcg cgtctggaga ccaatcgcgg
     5701 cattgtctcc ggcctggatc cggccacgca ccgcaggttc gtcacggcca tgacgaacgc
     5761 cgtcaagacg gtccaggagg ccgggtacca cgaggtggtc ctctgctccg aggcggcacg
     5821 gccgctggtg aagtccgcga cggcccggga gatcccgcac ctggcggtcc tgtccagcct
     5881 ggagatctcg gcggacgtga gcgtggagtc tctgggggag gttcgcatag aaacggatgg
     5941 aaagggataa cgggggaagg gccagcgcat gcaatacttc acgatccagg cgagatccaa
     6001 ccgggaggcg atcgagaaga tgaagagcac ctatggggag gacgccagga tcctcactca
     6061 tcgggccgtt cgcatgggcg ggtttctggg cctgttcgcc gccgaggggg tcgaggtcac
     6121 cggctacctc tccgacgagg gagtccggcg aaagaaggtg gaagtggaag aggagaaaaa
     6181 gaggatcctg gagagcgtca agaaagagca gaccctgcag ctcctcttga gggagatcca
     6241 ggggcttcgg gaatccatcc aggcccgtcc tcccgcgccg cccgagccgc ccgcggcgga
     6301 agagctcacc tccctcaagc agctggagga tctgctcgtg gcgaacgagt tcgctccgga
     6361 tttcgcctcg ggtctcgtca cccgggcgcg gcgcgagctg tcgctggaga ccctggagaa
     6421 cttcgccctc ctccagaacg cggccgtgga atggatcgga gaatgcatcg ccatcgatcc
     6481 gccgctgtcg gggcgctcgc cccgcgtcgt cgtgatcgtg ggaccgaccg gggtcggaaa
     6541 gacgaccacc atcgccaagc tggcggcgat ctacgggatc ggggacggtc ggaccccggc
     6601 ccgcaaggtg cggatcatta ccatcgacaa ttaccgcatc gcggccaaac agcagatcga
     6661 gacctacgcc gagatcatgc ggatcccggt caacctggtg gagagcgccg aggacctccg
     6721 caagaacctg gcggtctacc aggacaccga cctcgtgctg gtggacacca tcggccgcag
     6781 cccccgggac ctgtccaagc ttgccgagat gaaggagatc ctggcgacct gcggcggccg
     6841 cgcggcggtt tacctcgcgc ttgcggccac gaccaagggg agcgacacgg aggagatcct
     6901 tcgccagttc gagcccttcg ggtaccgctc cgtgatcctc accaagctgg acgagaccat
     6961 gcggatcggc aacatcgttt cctgcctgac gcgccaccgc aagtcgattg cctacctctg
     7021 cgacggccag ggggtccccc aagacctgga ggaggccagc gtggtacgat tgctcatgaa
     7081 tatggacggg ttccggatcg accgggacac catggagcgc cgcttcgcca ggccccgtgc
     7141 ggacactggc gatgcgcgag ggagaggggc ctgaaagaca tggcagacca ggcggagaag
     7201 ctcaggcagc tggtcagctc gagcgctgcc ggatcccagg gagcgggaac ggggccggag
     7261 gcgggttccg ccccggaggc gccgacccgc gtgaaagaga aatcgtcgcc ggcgcggcct
     7321 cccgagggtc gcgcggccac ccgcatcatc gccatctcca gcgggaaggg aggcgtcggc
     7381 aagaccaaca tcgcgatcaa ccttgcgctg gcgtacgcca aggcgggcaa gaaggtcatc
     7441 gtcatggacg cggacctggg tctggccaac gtcaacgtgg ttctcggggt gatcccccgc
     7501 tacaacctct accatctgat ccgcaagcag aagacgctgt cggaaatcct ggtcgatacc
     7561 agctatggga tccagatcat cgccggcgcc tcgggtttct ccaagatcgc caacctgagc
     7621 gaggaggagc ggcgggactt catcggtgag ctggcggcgc tctccaacgc ggacatcctg
     7681 atcatcgact gcgcggcggg ggtatccaac aacgtcatct ccttcatcgc cgccgcggat
     7741 gacgcgctca tcgtcaccac tccggagccg accgccatca cggacgcgta cgggatcatc
     7801 aagatcatcg ccacggagat cgagaacctg gacctgggtc tcaagctcgt ggtcaaccgc
     7861 gtcaagtccg tggtggaggg caaacgggtg gccgagcggg tgatcaatat cgccggccag
     7921 ttcctcaacc tcaaggtgga ctatctcggg ttcgtgttcg acgacccggt ggtccacacc
     7981 tccgtggtcc gacagaagcc gttcttcctc ctggagcccc gctcgcgcgc ttccgtgtgc
     8041 atccgccacc tggtgggacg gctggagaac tacgactatc gggacgggaa gggcatcccg
     8101 gtgttcctca agaagctctt cggaggcaaa tgaagccggt tcggagggac gtgacgcatc
     8161 gcccattgtt gactctaggt ctcgtttcac ctactgttag ccagggggga tcggtccggt
     8221 gatggagctc ttcagggagt acgggaagac cgccggcatc ttcgccgggg cggcatttct
     8281 gctttccctg ctggtgggcg ctctgtcgcg caatcccttc ggcgtggtcc ttctgcgcgc
     8341 cctgctgttc gggctcctgt tcgccctcct cgggggcggt taccggtacc tgtatctgcg
     8401 gttcctgtcg cagccttccg gctccggcgc ggagaaggcg gcggccgcct ccggttccgc
     8461 ggtagacatc gtgcttcccg aagagcctgc gacctacgag ccgtcggcct cgggagagga
     8521 cgcggaggct gtcgagccgg aggaggtagc ggaggccgag gcttccgggg aggaggcgcg
     8581 ccccgcgcag tcggtggccg gcgcgggacc gacggaggac tccggagatc tcctggatgc
     8641 ggagacggag ggggaatccg aggacgagct ccttcccgtc atggaggagg cggactccgg
     8701 gtccgccgca tcgggggacg ggcagtccgg actggatgag cttccggatc tcatggacct
     8761 ggacgcgccg gcaaggcccg gcgggcgggg ccgcgccggc gagcaacgcg gcgcggagcc
     8821 gcgaccggga gagcccgacg cggctgggtc ccggccggcc gcgccgggac ccggccgtcg
     8881 gcgggcgacg ccttctcccg gaacgacgcc ggaggaggcg gtccgtaacg cggtgagcga
     8941 agaggatccc gcgagcctgg cccgcgcgat ccgtacggtc ctgaagaggg atggcaaagg
     9001 atagcgcgga tatggccgag agtgtgtggg acgggaaatc cgaagaggac ctgtggcggg
     9061 tgtaccggcg cacaagagac cagaaggtgc gggactactt cgtccgccag tacgcgccgc
     9121 tggtaaagta cgtggcgggg aagatcgccc tggggatgcc gcagaacgtc gagttcgacg
     9181 atctggtggg attcgggatc ttcggtctct tcgacgccat cgacaagttc gatcccagca
     9241 agcacgtgaa gttcaagacc tacgccgtaa cccgcatccg cggggccatc ttcgacgagc
     9301 ttcggaccat cgactgggtg ccccggtccg tgcggcaaaa agcccgagag gtggaggaga
     9361 cggtccgcag gctggaatcc tccctgggcc gcgccgccac ggatgaggag atcgcttccg
     9421 gcatggagat ggaggtcaag gagttccagc agatgatgct ccgcatcagc gggacctcca
     9481 tgctgtctct ccacgacatc tggtatacgg gagaggacaa cgacaagatc tccatcgcgg
     9541 agagcatcga gagcccgggc agcctcaacc cggacaccat cgtggagaaa gaggagatca
     9601 agcgggtcat cgtggaggcc atcaaggagc tgccggacaa ggagaagaaa gtactggtgc
     9661 tatactatta cgaggacctc accctcaagg agatcgggga ggtgctcgaa gtcaccgaaa
     9721 gccgggtttc gcagcttcac accaaggcgg tcatgcggct gcgggccaag ctgaccagct
     9781 tcaaacgcgg aatcgtgtag gccagggatg cgtagcacgc cgcaggcgtg cggagggcgg
     9841 cagctgcgcg cgtagcgcgc agcgagagcc gccgaaggag ccgtgagggc agggatgccc
     9901 gacaggctcc tatgccaggg atgcccgaga ggctcccagg ggaaggggac atggccacgc
     9961 tgatcggact ggaggagctg cgggatctga tgcggaagca gtccgaggag gaccgcaaga
    10021 atcggaccat caccgtggag ggcccgagcg tggaagaggc gctgaagcgc gcctccatcg
    10081 agctgggggt gccggtccgc cggctggagt tcgaggtggc catccgcggc agcgacggga
    10141 tgctgggcat gggccgaaag ccgtggaagc tctccgtcta cgagaagtcc cagatggtga
    10201 agacggagca ggagtcccag gccgatgccc tgcgggagga ggagctgtcc gccgccgagg
    10261 cccagcggcc caaggaccgc ccgggggaag tcttcgtccg catccaggcg gacggggtct
    10321 acctcaaggt gactcgtccc cagggccgag gtccgaaggc gaccgagctc atggccctgg
    10381 agcggctcag cctgcgcggt gtgtcccggt tcgatgaggg gctcgtttct cgcgtggtca
    10441 agcacgcgga cggcgagtac gttcacgttg ccgacgtcca gtacaacccc aacaacgatt
    10501 ccacggtttc catggacatt accgacggct cgatgaaggc cgtggtcgtc ctctcggagc
    10561 cgggccccgg cggggcggac atctcggcgg agtacctccg gtcggtgctg cagtccaacg
//
LOCUS       DASRZS010000131         4413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1606876, whole genome shotgun sequence.
ACCESSION   DASRZS010000131 DASRZS010000000
VERSION     DASRZS010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606876"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..2077
                     /gene="dnaK"
                     /locus_tag="VFH83_03255"
     CDS             152..2077
                     /gene="dnaK"
                     /locus_tag="VFH83_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET6485410.1"
                     /translation="MGRVIGIDLGTSNSAAAVMEGGRPVIIPSAEGAGVSSGKAFPSF
                     VAFTKESDRLVGEPARRQAAINPDGTIYAAKRKMGSDYKFKVYGKEYSPQQISAFILQ
                     KIKQDAESYLGDTVEEVVITCPAYFDDNQRTATKDAGEIAGLKVLRIINEPTAACLAY
                     GLEKAGKELKILVFDLGGGTLDVTIMEMWKEGGFKVVATSGDTQLGGTDMDNTFVDYV
                     VKEFQRSTGIDLKKDTMAMQRVREAAEKAKVELSSTLTTDINLPFITADATGPKHLTM
                     TINRAKLEELVQPIVARCRGPLETALRDATAAFSKDGVQFGAGGADIDKIIMVGGPTR
                     MPIIQKFVEDFFGKKIERGVDPMECVALGAAIQAAIIKGEVKDVLLLDVTPLSLGIET
                     LGGVSTKLIERNTTIPTRKSEIFSTAADNQTEVTIRVLQGERQMAGDNVELGRFNLVG
                     IPPAPRGVPQVEVTFDIDANGIVSVSAKDLGTGKQQSIKITAPKKLSKEEIEKMVHQA
                     EQFASDDAKKKEEVEAINQADTLAYSVEKSLKDYGDKISQAERAEIEAKLNDLKNAIK
                     DKNPERIKKGMDELTKASHKLAEEVYKQAGPKGPSGQDAGPQPGGDGGAGAAGGGGNG
                     KKGDDVIDAEFREEKKQ"
     gene            2116..3234
                     /gene="dnaJ"
                     /locus_tag="VFH83_03260"
     CDS             2116..3234
                     /gene="dnaJ"
                     /locus_tag="VFH83_03260"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET6485411.1"
                     /translation="MSTKRDYYEILGVKRNATHEELKKVYRELALRYHPDRVAADQKK
                     EAENRFKEISEAYAVLSDPQKRALYDQYGHAGVDQKYANEDLYRGTDFNGVFEGLGDL
                     GLGGFFENLFGGGGGFDLFGRGRRGRGGAQEQGGKGRDLEVAVGVTLEEAYRGTEKKV
                     TVPRYDKCSVCGGNGAKPGTGMTTCPDCRGTGRTTVSGGIFQMGQTCKRCGGSGSIPQ
                     TPCDTCHGEGRVRVSRTLTVTVPPGVDNGSRLRMKGEGEAGTRGTGDLYLVVELQRHP
                     TFERQGNDVLTELTVSLPRALLGTEVSVPTLDGGVAMKIPAGTQSGSVFRLKGKGMPE
                     LRGQGVGDELVKVNVEIPRRLSDRQRALVEELAKALGD"
     gene            3285..4232
                     /locus_tag="VFH83_03265"
     CDS             3285..4232
                     /locus_tag="VFH83_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6485412.1"
                     /translation="MVELNEVRKSYGALTAVDGISFRAEPGEIFGLLGPNGAGKSTTI
                     KMIMNILVPDSGSIRLNGNPIREEDKDRIGYLPEERGLYRKVKVGEMLLYLASLKNAD
                     PVAASRRLDEWLERFELTEWKNRTPDALSKGMAQKAQFIAAVLHDPQFIFLDEPFTGL
                     DPVSTDVLREVVLELGGKGKTILFSTHNMEVAERLCSRIFVIDHGRELLSGTLAEVKA
                     RYGQRSIVVEFDGQIDWSRLSGMVANVTRYPRWVEIELAADAQPQELLKRLVDQVAVT
                     RFEVMAPSLHKIFVQMVAEAGNRVAEAGNRVAEAGEARS"
     gene            4229..>4413
                     /locus_tag="VFH83_03270"
     CDS             4229..>4413
                     /locus_tag="VFH83_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485413.1"
                     /translation="MSGKLARIIRMEFKLTAANKVFIVLTILGPFLIAAVTVLPTMLA
                     TSGSTLGSPEMRVAISGP"
BASE COUNT          883 a         1378 c         1525 g          627 t
ORIGIN      
        1 tgacttagcg tcggtcccta tttacatttc catcgaagct gaatgtctct cgcaaaatgc
       61 gaaaaggacc aaggataggt ttcaccggac ggcaacccgc gggagtttcc gcgtgtccgg
      121 aaagcgagag agcaaccaaa ggagcaacac tatgggacgg gtaatcggca tcgacctggg
      181 gacctcgaac tccgccgctg ccgtcatgga gggcggccgg ccggtgatca tcccctccgc
      241 ggagggagcc ggcgtctcct cggggaaggc gttcccctcg ttcgtcgcgt tcaccaagga
      301 gagcgatcgc ctggtgggcg agccggcgcg ccgtcaggcg gccatcaacc ctgacgggac
      361 catctacgcg gccaagcgga agatggggtc ggactacaag ttcaaggtct acgggaaaga
      421 gtacagcccc cagcagatct ccgcgttcat cctgcagaag atcaagcagg acgcggagtc
      481 gtacctgggg gacacggtgg aggaggtggt catcacctgc cccgcctatt tcgacgacaa
      541 ccagcgcacc gccaccaagg acgccggcga gatcgccggg ctcaaggtgc ttcgcatcat
      601 caacgagccg acggcggcct gtctggcgta cgggctggag aaggccggca aggagctcaa
      661 gatcctggtg ttcgacctgg gcggtggaac cctggacgtc accatcatgg agatgtggaa
      721 agagggcggg ttcaaggtcg tggccaccag cggggacacc cagttgggcg gcaccgacat
      781 ggacaacacg ttcgtggact acgtggtgaa ggagttccag aggtcgaccg gcatcgacct
      841 gaagaaggac accatggcca tgcagcgcgt ccgtgaggcc gccgagaagg ccaaggtgga
      901 gctctccagc accctgacca cggacatcaa cctgccgttc atcacggcgg acgccacggg
      961 ccccaagcac ctcaccatga ccatcaaccg ggccaagctg gaggagctgg tgcagcccat
     1021 cgtggcccgc tgccgcggcc cgctggagac cgcgcttcgg gacgccacgg ccgccttctc
     1081 caaggacgga gtccagttcg gcgccggcgg tgcggacatc gacaagatca tcatggtcgg
     1141 cggacccacc cgcatgccca tcatccagaa gttcgtcgag gacttcttcg gcaagaagat
     1201 cgagcgcggc gtggatccca tggagtgcgt ggccctgggc gcggccatcc aggcggccat
     1261 catcaagggc gaggtcaagg acgtgctcct tctggacgtc acgcccctct ccctggggat
     1321 cgagacgctg ggcggcgtca gcaccaagct catcgagcgc aacacgacca ttcccacgcg
     1381 caagagcgag atcttctcca cggcggcgga caaccagacg gaggtcacca tccgcgtgct
     1441 ccagggggag cgccagatgg ccggagacaa cgtggagctg ggacgcttca acctggtggg
     1501 gatccctccg gcgcctcggg gcgtgcctca ggtcgaggtg acgttcgaca tcgacgccaa
     1561 cggcatcgtg agcgtctccg cgaaggacct gggcaccggc aagcagcagt ccatcaagat
     1621 cacggcgccc aagaagctgt ccaaggaaga gatcgagaag atggtccacc aggcggagca
     1681 gttcgcctcg gatgacgcca agaagaagga agaggtcgag gcgatcaacc aggcggacac
     1741 gctggcgtac tcggtggaga agtccctcaa ggactacgga gacaagatca gccaggcgga
     1801 gcgcgcggag atcgaggcca agctcaacga cctgaagaac gcgatcaagg acaagaaccc
     1861 ggaacgcatc aagaagggta tggacgagct caccaaggcg tcccacaagc tcgccgagga
     1921 agtgtacaag caggccggtc ccaaaggccc gtccggccag gacgcgggtc cccagcccgg
     1981 cggtgacggc ggggccgggg ccgcgggcgg gggcggcaac ggcaagaagg gcgacgacgt
     2041 catcgacgcc gagttccggg aagagaaaaa gcagtaacgg aggcgggcgt cggggcaacc
     2101 gacgcccgca catcaatgtc gaccaaacga gactactacg agatcctcgg ggtcaagcgc
     2161 aacgcaaccc atgaggagct gaagaaggtc tatcgcgagc ttgcgctccg ctaccacccg
     2221 gaccgggtcg ccgcggacca gaagaaagag gcggagaacc ggttcaagga gatctccgag
     2281 gcatatgccg tcctctcgga cccgcagaag cgcgcgctgt acgaccagta cggtcacgcg
     2341 ggcgtggatc agaagtacgc caacgaggac ctctaccggg gcaccgactt caacggcgtc
     2401 ttcgagggcc tgggcgatct tggccttggg ggcttcttcg agaacctctt tggcggaggc
     2461 ggcgggttcg acctcttcgg ccgtggacgg cgggggcggg gcggcgctca agagcagggc
     2521 ggcaagggtc gcgacctgga ggtggccgtc ggcgtgaccc tggaggaagc ctaccggggc
     2581 acggagaaga aggtcacggt cccccgatac gacaagtgct ccgtctgcgg cggaaacggc
     2641 gcgaagcccg gcaccggtat gaccacctgc ccggactgcc gcggcaccgg tcgcaccacc
     2701 gtgtccggcg gcatcttcca gatgggccag acgtgcaagc gctgcggcgg ctccggttcc
     2761 atcccgcaga cgccgtgtga tacgtgccac ggagagggac gcgttcgggt ctcgcggacc
     2821 ctcaccgtca cggtcccgcc gggcgtggac aacgggtccc ggctccgcat gaagggggag
     2881 ggggaggccg gcacgcgcgg cacgggcgac ctctacctgg tggttgagct gcagcggcat
     2941 cccaccttcg agcggcaggg caacgacgtc ctgacggagc tcaccgtcag ccttccccgg
     3001 gccctgctcg gcaccgaggt cagcgttccc acgctggacg gcggcgttgc catgaagatc
     3061 cccgccggaa ctcagagcgg gagcgtgttt cgtctgaagg gcaagggcat gccggagcta
     3121 cgcggccagg gcgtggggga cgagctggtc aaggtgaacg tcgagatccc gcgccgtctg
     3181 tcggaccgac agcgcgcgct ggtggaggag ctcgccaagg ccctggggga ctgacccgcc
     3241 gtccggtccg ccgatggtgc ggcccgctga caggagaaaa gtgaatggta gagctgaacg
     3301 aggttcgcaa gagctacgga gccctgaccg ccgtggacgg gatcagcttc cgggccgagc
     3361 cgggggagat cttcgggctc ctgggcccca acggcgccgg caagtccacc accatcaaga
     3421 tgatcatgaa catcctggtc ccggattccg gaagcattcg gctgaacggc aatcccatcc
     3481 gtgaagagga caaggatcgc atcgggtacc ttccagaaga gcggggcctg taccgcaagg
     3541 tgaaggtggg ggagatgctc ctctatctgg ccagcctcaa gaacgcggac ccggtggcgg
     3601 catcgcggag gctggacgag tggctggagc gcttcgagct cacggagtgg aagaaccgga
     3661 cgccggacgc gctctccaag gggatggccc agaaggccca gttcatcgcc gccgtcctcc
     3721 atgaccctca gttcatcttc ctggacgagc cattcacggg gctggacccc gtgagcaccg
     3781 acgtgctccg agaggtggtc ctggagctcg gcggcaaggg gaagaccatc ctcttctcca
     3841 cgcacaacat ggaggtcgcc gagcggctgt gctcgcggat cttcgtgatc gaccacggcc
     3901 gcgagcttct ctccgggacg ctcgcggagg tgaaggcgcg ctacggccag cgcagcatcg
     3961 tagtggagtt cgacggccag atcgactgga gccgactctc cggaatggtc gccaacgtga
     4021 cgcggtatcc ccggtgggtg gagatcgagc tggccgccga tgcccagccg caggagcttc
     4081 tgaagcggct ggtggaccag gtggcggtca cgcggttcga ggtgatggcc ccgtccctgc
     4141 acaagatctt cgtgcagatg gtcgccgagg ccggcaaccg ggtcgccgag gccggcaacc
     4201 gggtcgccga ggccggggag gcgcgctcat gagcggcaag ctggccagga tcatccgcat
     4261 ggagttcaag ctcacggctg ccaacaaggt cttcatcgtc ctcaccatct tggggccctt
     4321 cctgatcgcg gcggtcacgg tcctgcccac gatgttggca acatcgggat cgaccttggg
     4381 aagccccgag atgcgggtgg ccatctccgg tcc
//
LOCUS       DASRZS010000132         5647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4304346, whole genome shotgun sequence.
ACCESSION   DASRZS010000132 DASRZS010000000
VERSION     DASRZS010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4304346"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VFH83_03275"
     CDS             complement(<1..876)
                     /locus_tag="VFH83_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET6485414.1"
                     /translation="MSDKRLPFDRAQIERIIQTHPTPFHIYDERGIRENARALLKGFS
                     WAEGFREFFAVKACPNPHVVQMLKQEGFGADCSSLAELVLSERVGLRGETIMFTSNDT
                     PSAEYVKAKELGAIINLDDISHIRFLKEHAGLPELISMRYNPGRLREGNAIIGKPEEA
                     KYGFTREQLFQGYRQVRDQGVKRFGLHAMIVSNELNLEYHLETARMLFDLVADLSREL
                     GIRFEFVNLGGGFGIPYRPDQTPLDWAAVGAGIRQVYDERIRSRGLHPLRLCTECGRA
                     ITGPYGFLVTRVLHLK"
     gene            complement(940..1584)
                     /locus_tag="VFH83_03280"
     CDS             complement(940..1584)
                     /locus_tag="VFH83_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6485415.1"
                     /translation="MDGPRVHHVRHLTPSAFVVRFDRGGMLFEPGQHLNLGLRGALAM
                     REYSIYSGAADDFLEVLVREVEGGAVSGALKRCSPGDQLSMEGPYGIFVTEERDRSTA
                     PFLFVGSGTGIAPFHSIVRSYPEIRYRLLHGVRTEQERFDFETFERSRYLCCASRSSR
                     GDYAGRVTAWLREHPVDPDSRCYLCGNSDMIYEAFAILRGQGVPRERLFAEVYF"
     gene            1665..2684
                     /gene="moaA"
                     /locus_tag="VFH83_03285"
     CDS             1665..2684
                     /gene="moaA"
                     /locus_tag="VFH83_03285"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011252030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET6485416.1"
                     /translation="MSAIQDRLARPLHDLRISVIDACNFRCPYCMPAAVFGESYQFLK
                     PSELLTFEEITRLARLIAGLGAVKIKLTGGEPLLRPWLADLVRRLSEVPGIEDLALIT
                     NGAHLARMARPLRAAGLRRLTVSLDSLDEDTFSRMNGMAHRVRPVLDGIAAAEDAGFT
                     GIKLNAVVIRGMNDHQPPDLVRRFRGTGHIVRFIEFMDVGNRNGWRADLVVPSRETLE
                     RISREFPVEPVEPNYRGEVASRYRLLDGSGEIGFISSVSQPFCKDCTRLRLSADGTLY
                     TCLFAVKGLDIREPLRGGASDAELLSLLSDVWKRRTDRYSEERAARLSAAAGARKVEM
                     YTVGG"
     gene            2688..2939
                     /locus_tag="VFH83_03290"
     CDS             2688..2939
                     /locus_tag="VFH83_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET6485417.1"
                     /translation="MAGQKSVRVEYFAALREATGVREEAIRSAAETAADLYAEVAAAH
                     GFRFDRSSLGVAVNDAIAPWTTRLRDGDRVAFLTPFAGG"
     gene            2952..3398
                     /locus_tag="VFH83_03295"
     CDS             2952..3398
                     /locus_tag="VFH83_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET6485418.1"
                     /translation="MSDGALFELWDHSIDGSTLARSMADSHAGALVIFEGRVRDTNDG
                     KTVVSLEYEAYPKLSLNEGNAIVREALRGVVGARCVHRVGRLSVGDVAVWVGVVAEHR
                     GEAFQACRFIIDEVKRRVPIWKKEHYADGSSVWIGMGERSVSSKET"
     gene            3462..4445
                     /locus_tag="VFH83_03300"
     CDS             3462..4445
                     /locus_tag="VFH83_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017287846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6485419.1"
                     /translation="MTHILVEDLRKTFRVAVRRSGTLGALAGLVHRRTRTVHALDGVS
                     FRIDQGELVGYIGPNGAGKSTTVKILGGILVPDSGRCEVMGRVPWKNRIENVSRIGVV
                     FGQRTQLWWDLPVIESFDLLRDIYRVPARQYRETLEELVELLELRSLLDVPVRQLSLG
                     QRMRCDLAASLLHSPSLLYLDEPTIGLDAVSKLAVRDFVQRQNRARGTTVILTTHDMD
                     DIEALCNRIILIHQGAILYDGTLDGLRAQVTNERQLIIDLKDGSLRVSDPDAISVEQE
                     GHRARIRFDPSRIAAPELIARMSARFPVADLTVENPPIEEIVARLYRRLGG"
     gene            4449..5240
                     /locus_tag="VFH83_03305"
     CDS             4449..5240
                     /locus_tag="VFH83_03305"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET6485420.1"
                     /translation="MGAYRSLFAARFRALLQYRGAAIGGMACQIFFGLILVMVYEAFY
                     RSGSRSQPMTISQVRSYVWLGQAFLAMLPWNVEGEQRLMIRTGSVAYELLRPVRLYSL
                     WFARSLAWRTAPTVMRAVPILVVAMAFFGLTLPPSPAAAGAFVLVMGGALLLSVAMTT
                     LFTIFLMWTVSGDGFTMLFYAMVILLSGLNIPIPLFPVWLRPIVEALPFRCLVDVPYR
                     MYVGYADPGSAFGLLALQLAWSGVTVGVGFLLMRGGLRRLTIQGG"
     gene            5257..>5647
                     /locus_tag="VFH83_03310"
     CDS             5257..>5647
                     /locus_tag="VFH83_03310"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET6485421.1"
                     /translation="MASDLKLYARYLGISVRSQMEYRASFIMLSVGQFLVTIIEFASI
                     VLLFDRFGGIQGWTLPEVGFFYGLINTAFAITDAAARGFDVFGAMVKGGDFDRLLLRP
                     RGTALQVAGTELTLRRVGRLLQGSLVLA"
BASE COUNT          867 a         1878 c         1903 g          999 t
ORIGIN      
        1 cttcaggtgg aggacgcggg tcaccaggaa tccgtagggg ccggtgatgg cgcgcccaca
       61 ttccgtgcac agccgaagcg ggtgcaggcc gcgggagcgg atccgctcgt cgtagacctg
      121 tcggattccc gcgcccaccg ccgcccagtc cagcggcgtc tggtcgggac ggtatgggat
      181 gccgaacccc ccgcccaggt tcacgaactc gaagcggatc cccagctccc gggagaggtc
      241 cgccaccagg tcgaagagca tccgcgcggt ctccaggtga tactcgaggt tgagctcgtt
      301 ggagacgatc atggcgtgca ggccgaagcg cttgacgccc tggtcccgca cctgccgata
      361 cccctggaag agctgctccc gcgtgaagcc gtacttggcc tcctcgggct tgccgatgat
      421 cgcgttccct tcccgcagcc gcccggggtt ataccgcatg ctgatgagct cgggcagtcc
      481 ggcgtgctcc ttgaggaatc ggatgtggct gatgtcgtcc aggttgatga tcgcgcccag
      541 ctccttcgcc ttcacgtact ccgcggaagg cgtgtcgttg gacgtgaaca tgatcgtttc
      601 gcctctcagc ccgaccctct cggacaggac cagctcggcc agggagctgc agtccgcgcc
      661 aaacccctcc tgcttcagca tctggacgac atggggattg gggcaggcct tgaccgcgaa
      721 gaactcgcgg aacccctccg cccaggagaa tcccttgagg agcgcccggg cgttctcccg
      781 aatgccccgc tcgtcgtaga tgtggaaggg cgttggatgg gtctggatga tccgctcgat
      841 ctgcgcgcgg tcaaacggga gcctcttgtc cgacattcgc atctcctgtg gcgcaggata
      901 acgagcgggt gccaaccgtg tccaccggtg gcccctcgct cagaagtaca cttccgcgaa
      961 gaggcgctct cgggggacgc cctgcccgcg gaggatggcg aaggcttcgt agatcatgtc
     1021 gctgttgcca cagaggtaac accgggagtc cggatccacg ggatgctccc gcaaccacgc
     1081 ggtgacccgg cccgcgtagt cgccccggga agaccgcgac gcgcagcaaa ggtagcgcga
     1141 tcgctcgaac gtctcgaagt cgaaacgctc ctgctcggtc cgaacgccgt gaagcagccg
     1201 gtagcggatc tcggggtaac ttcgcacgat ggagtggaac ggggcgatac cggtcccgga
     1261 tcccacgaag aggaacggcg cggtcgatcg gtcccgttcc tccgtcacaa agatgccgta
     1321 cggaccctcc atcgaaagct ggtccccggg gctgcatcgt ttcagcgcgc cggacaccgc
     1381 cccgccctcg acttcccgga ccagcacttc caggaaatcg tcggcggcgc ccgagtagat
     1441 cgagtactcc cgcatggcca gggcgccgcg cagcccgagg ttcaggtgct gacccggctc
     1501 gaagagcatg ccgccccggt cgaatcgcac gacgaatgcg ctgggggtga gatgccgtac
     1561 gtggtgaacc cgcggcccgt ccacaacggc tatttcgtcg gcgcgggacg aacctgtcaa
     1621 ccgtcgttga cggcaatgcg acgaaaccct atattccgat tgcattgagt gccatccagg
     1681 accgccttgc ccgcccgctt cacgacctgc gcatctcggt gatcgacgcc tgcaacttcc
     1741 gctgtcccta ctgcatgccg gcggccgtgt tcggggaatc ctaccagttc ctcaaaccct
     1801 cggagcttct caccttcgag gagatcaccc ggctggcccg gctcattgcg gggctcggag
     1861 cggtcaagat caagctcacg ggaggcgagc cgctcctgcg cccgtggctt gcggacctgg
     1921 tgcggcggct ctcggaggtg ccggggatcg aggacctcgc gctgatcacc aacggcgcgc
     1981 acctggctcg gatggcccgg ccgcttcgag ccgccggcct caggcgcctc acggtgagtc
     2041 tcgattccct ggatgaggac acgttctccc gcatgaacgg catggcccac cgggtgcggc
     2101 cggtgctgga cggcatcgcg gcggccgagg acgccggatt caccggcatc aagctcaacg
     2161 ccgtggtcat ccgaggcatg aacgaccatc agccgccgga cctggttcgg cggtttcgcg
     2221 ggacggggca catcgtgcgg ttcatcgagt tcatggacgt gggcaaccgc aacggctggc
     2281 gcgcggacct cgtggtccct tctcgggaga ccctggagcg gatctcccgg gagttccccg
     2341 tggagccggt ggagcccaac taccgcgggg aggtggcatc ccgctaccgg ctgctcgacg
     2401 ggagcggaga aatcggattc atctcctcgg tcagccagcc cttctgcaag gactgcaccc
     2461 ggcttcgcct ctccgcggac ggcacgctct acacgtgtct cttcgcggtg aagggcctgg
     2521 atatccgtga gcccctgcgc ggcggggcgt cggacgccga gctcctgtct ctcctctccg
     2581 acgtgtggaa gcgtcggacg gaccggtact cggaggagcg cgccgcgcgg ctcagcgcgg
     2641 cggccggcgc gcgcaaggtg gaaatgtaca cggtgggtgg ttagcgaatg gcaggacaga
     2701 aatcggttcg cgtggagtac ttcgcggcgc ttcgcgaggc caccggagta cgcgaggaag
     2761 cgatccgcag cgccgccgag accgccgcgg acctgtacgc cgaagtggcc gccgcgcacg
     2821 ggtttcggtt cgaccgctcg tccctggggg ttgccgtgaa cgacgccatc gccccgtgga
     2881 ccacgaggct ccgcgacggc gatcgggtgg catttctcac tccctttgcc ggcggctgac
     2941 ggagcggcgg cgtgagcgac ggcgcgctct tcgagctctg ggaccattcc atcgacgggt
     3001 ctaccctggc ccggtccatg gcggacagcc acgccggcgc gctggtcatc ttcgaaggtc
     3061 gcgtgcggga cacgaacgac ggaaagaccg ttgtcagcct ggaatacgag gcctatccga
     3121 agctgtccct gaacgagggg aacgccatcg tacgggaagc gctgcgcggc gtggtcggcg
     3181 cccggtgcgt acaccgcgtg ggccgtctct ccgtgggaga cgtcgcggtt tgggtgggcg
     3241 tggtcgccga gcaccgcggg gaagcgttcc aggcgtgccg gttcatcatc gacgaggtga
     3301 agcgtcgggt ccccatctgg aagaaggaac actacgcgga cggcagctcg gtctggatcg
     3361 ggatggggga gcgatcggtc tcttcgaagg agacgtaggt acccgccgcg agccgtcttg
     3421 gctcgaaccc gttttcgtgc tcatcatctc ccatcaacac catgacccat atcctggtcg
     3481 aggatcttcg gaagaccttt cgcgtcgccg tgcggcgctc cgggactctg ggagctctcg
     3541 cgggactggt gcaccggcgg acgaggaccg tccacgccct ggacggggtg agcttccgca
     3601 tcgatcaggg cgagctggtc gggtacatcg gtcccaacgg ggccggcaag tccaccacgg
     3661 tgaagatcct gggcgggatc ctggtgccgg actcgggccg ctgcgaggtg atgggccggg
     3721 tgccctggaa gaaccggatc gagaacgtgt cccggatcgg cgtggtgttc gggcagcgaa
     3781 cgcagctctg gtgggacctg ccggtgatcg agtccttcga tctcctgcgc gacatctacc
     3841 gggtccccgc gcgccagtac cgagagaccc tggaggagct ggtggagctc ctggagctgc
     3901 gaagcctgct ggacgtcccg gtccgccagc tcagtctggg ccagcggatg cggtgcgacc
     3961 tggcggcctc cctgctgcac tccccgtccc tcctctacct ggatgagccg accattgggc
     4021 tggacgcggt gtccaagctg gccgtgcgcg atttcgtcca gcgccagaac cgggcgcggg
     4081 ggaccacggt catcctcacc acccacgaca tggacgatat cgaggccctg tgcaaccgga
     4141 tcatcctgat ccaccaggga gccatcctct acgatgggac gctcgacggc cttcgcgccc
     4201 aggtgaccaa cgagcggcag ctcatcatcg acctcaagga cgggagcctg agggtcagcg
     4261 acccggacgc catctccgtg gagcaggaag ggcaccgcgc gcggatccgc ttcgacccga
     4321 gccggatcgc tgccccagag ctgatcgctc gcatgagcgc ccgcttcccg gtggcggacc
     4381 tgacggtgga gaacccgccc atcgaggaga tcgtggcccg gctgtatcgc cggctgggcg
     4441 gctgacccgt gggcgcctat cggtccctgt tcgcggctcg cttccgcgcc ctgctccagt
     4501 accggggggc cgccatcgga gggatggcct gccagatctt cttcggattg atcctggtga
     4561 tggtgtacga ggcgttctac cggtccggaa gccggagcca gcccatgacc atctcccagg
     4621 tccgatccta cgtctggctg ggacaggcgt tcctggcgat gctcccgtgg aacgtcgagg
     4681 gggagcagcg gctcatgatc cggaccggct cggtggccta cgagctcctg cgccccgtgc
     4741 ggctgtactc cctctggttc gcccggtccc tggcgtggcg gaccgccccc acggtcatgc
     4801 gggccgtccc gatccttgtc gtcgccatgg ccttcttcgg gctgaccttg cctccgtccc
     4861 ccgccgcggc tggggcattc gtcctggtca tgggcggcgc gctccttctg agcgtcgcca
     4921 tgaccacgct cttcacgatc ttcctgatgt ggaccgtgtc gggcgacggg ttcaccatgc
     4981 tcttctatgc gatggtcatt ctgctctccg ggttgaacat cccgatcccg ctctttccgg
     5041 tctggctgcg gcccatcgtg gaagcccttc cgttccgctg cctcgtggac gtcccctacc
     5101 ggatgtacgt gggatacgcc gacccgggct ccgcgttcgg actcctggcg ctgcagcttg
     5161 cctggagcgg cgtgacggtc ggcgtcgggt tccttctgat gcgcggcggg cttcggcgtc
     5221 tgaccatcca gggagggtga cggcgatgga ggcgacatgg cgagcgatct gaagctctac
     5281 gccagatacc tcgggatctc cgtgcgcagt cagatggagt accgcgcctc gttcatcatg
     5341 ctcagcgtcg gccagttcct ggtgaccatc atcgagttcg cgtccatcgt gctgctcttc
     5401 gatcggttcg gaggcatcca ggggtggacg ctccccgagg tggggttctt ctacggtctc
     5461 atcaatacgg ccttcgcgat cacggatgcc gccgcgcgcg gcttcgacgt gttcggggcc
     5521 atggtgaaag gtggagactt cgaccgactt ctccttcggc cgcgcggcac cgcgctccag
     5581 gtagcgggca ccgagctcac gcttcgccgg gtgggaaggc tcctgcaggg atcgctggtg
     5641 ctggcgt
//
LOCUS       DASRZS010000133         4634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5073739, whole genome shotgun sequence.
ACCESSION   DASRZS010000133 DASRZS010000000
VERSION     DASRZS010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5073739"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFH83_03315"
     CDS             complement(<1..209)
                     /locus_tag="VFH83_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET6485422.1"
                     /translation="MRRTELIMGMPITVVIPERERMDTPVPGTRFITLSQAIEAVFNR
                     FRAVDERFSPYKPDSETCRIDRGEL"
     gene            complement(206..571)
                     /locus_tag="VFH83_03320"
     CDS             complement(206..571)
                     /locus_tag="VFH83_03320"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HET6485423.1"
                     /translation="MKTGIKTGITAAVLASAIGANAFAAGHALSNGTFTSRREYAYYG
                     YVRLKAVVKNGQLTDVQMVEYPSDNRTSRYINSIALPYLIQEAVQAQSSSVDLISGAT
                     FTSEAFTKSLEEALKAAGA"
     gene            complement(723..1757)
                     /locus_tag="VFH83_03325"
     CDS             complement(723..1757)
                     /locus_tag="VFH83_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6485424.1"
                     /translation="MTLLRPFGARDTASRADPFTTARIKLTLLYLALITVIVVVLSFS
                     LYSLHAGEVARSERVRREMVREREGLAELSPNVEDYLEELGRTVLIAGGLTIVVGGAL
                     SYLLAALTLRPIKRSVENEQLFFANAAHDLRTPLSVMRTEAEVALRSRTFGVSEARRA
                     LTSSLEEIGRMSTMVEQMLDLARRRSGAPGQRRFGPVDIGALVGAAASRMGRLASDKG
                     VDLRVEAVAPARIHGDEGAVQRAVVNVLENAITYTPSGGTITVVVKHQGGHVVVSVQD
                     TGIGIAPQDLPRITDPFHRGDQARRAHAGGAGLGLTIVKATMDYHRGTLQASSRPGQG
                     TTISLHFPAA"
     gene            complement(1708..2382)
                     /locus_tag="VFH83_03330"
     CDS             complement(1708..2382)
                     /locus_tag="VFH83_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6485425.1"
                     /translation="MRILVIEDNEKLAASVKTGLEQEGYAVDVALDGEEGLLLVDSLK
                     NGYDAVILDLMLPRKDGLSVCRAMRAHGEQAPVLMLTARDGVRDRVGGLDAGADDYLT
                     KPFAFEELAARLRALLRRPRRAPDVVLSVGDIELDSNTHEVKVLGKAVVLTSKEFRLL
                     ELFLRLPRHVLSRDQITNSLWDQEFDGGSNVVEVHIRNLRRKLEEAGKHDPIETVRGA
                     GYRLKG"
     gene            complement(2391..2708)
                     /locus_tag="VFH83_03335"
     CDS             complement(2391..2708)
                     /locus_tag="VFH83_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485426.1"
                     /translation="MDPLDSADILVQGSGRVLDRPAVGGSRTTAAAAPSPRAQPADAV
                     GSAPRLTILKAEQLLQIVATAQPTPISRQIAAAAYVMESEAQQELVKTRGISSETGRE
                     WFA"
     gene            2912..3490
                     /locus_tag="VFH83_03340"
     CDS             2912..3490
                     /locus_tag="VFH83_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011868797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6485427.1"
                     /translation="MAVAEGLGRKVRTVRESRKLSRQEMAERAALPAELVAQIEGDEL
                     VPSLSLLLRIARVLGVRLGTFLDDQENVGAVVTRSGELPRVTRFSSRIHAGPSSDLDF
                     HSLAVNKSGRHMEPFLIDIHPSSAESVQPSSHEGEEFIHVFEGEVEVLYGKDTYRLGV
                     GDSIYYDSVVSHHVHCSGGDTARILAVVYAPL"
     gene            3506..>4634
                     /locus_tag="VFH83_03345"
     CDS             3506..>4634
                     /locus_tag="VFH83_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011170510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6485428.1"
                     /translation="MQFLDMTLGQMLEKQAAAYPDRDFMVYADRDLRFTYRQFNQRVD
                     RLAKGLLAIGLHKGDHLGIWATNVPDWLTVLFATAKIGVVLVTVNTAYRTHELEYLIQ
                     QSDMDTLCLINGFRDSDYVAMVNELVPELKTCQRGHLCSERFPRLRNVIFLGPEKHRG
                     MFSTPEVLLLGEQVRDEELERASSGLSSAEVINMQYTSGTTGFPKGVMLTHNNILNNG
                     FQIGERQRFTEQDRVCLPVPLFHCFGLVLGIMAIYTHGAAAVVLEAFDPVMALAAVQK
                     EKCTALYGVPTMFIAELNHPMFSMFDTSSLRTGIMAGSPCPIETMKQVIETMHAGEIT
                     ICYGLTEGSPVMTQTSTDDSVDLRVSTVGRALPRIEVRIVDP"
BASE COUNT          782 a         1544 c         1515 g          793 t
ORIGIN      
        1 tcgagctctc cgcggtcgat ccggcaggtc tcgctatccg gcttgtaggg actgaaccgc
       61 tcatccacgg cgcggaaccg gttgaacacc gcctcgatgg cctggctcag cgtgatgaac
      121 cgggtgccgg gaacgggggt atccatccgc tcgcgctcgg ggatgacgac ggtaatgggc
      181 atccccatga tgagctcggt gcgtctcatg ccccggccgc cttcagcgcc tcctccaggg
      241 acttggtgaa cgcctcgctg gtgaacgtgg caccgctgat caggtccacg ctggagctct
      301 gcgcctgcac ggcctcctgg atcaggtagg gcagcgcgat gctgttgatg taccgcgaag
      361 tgcggttgtc gctcgggtac tccaccatct ggacgtcggt gagctgcccg ttcttgacga
      421 ccgccttcag gcgaacgtag ccgtagtagg cgtactcccg ccggctggtg aacgtgccgt
      481 tggacagcgc gtgtcccgcg gcaaaggcgt tggcgccgat ggccgaggcc agcacggcgg
      541 ccgtgatccc tgtcttgatt cctgttttca tctcgtcctc tgttggcttg atgttcggag
      601 ctcaagatga agagaagctg aagcagcggg agataacgga aagttctttc cgaatgggtc
      661 ggcgcgacgc ggaccttctt tccggcgtcc ggcgtccggc gtccggcccc cggcggcggc
      721 cttcaggccg ccgggaagtg gagggaaatg gtagtgccct gtccggggcg gctggacgcc
      781 tggagggtcc ctcggtggta gtccatcgtg gctttcacga tcgtgagccc cagccccgcg
      841 ccgccggcgt gcgcccttcg cgcctggtcg ccccgatgga acggatccgt gatgcgcggc
      901 aggtcctgcg gcgcaatgcc gatcccggtg tcctggacgc tgaccacaac gtggccgccc
      961 tggtgcttca ccaccacggt gatggttccg ccggacggcg tgtaggtgat ggcattctcc
     1021 agcacgttga ccacggcgcg ctgcacggcg ccctcgtccc cgtggatccg ggcgggtgca
     1081 actgcctcca cgcggaggtc cactcccttg tccgaagcga gccggcccat gcgcgacgct
     1141 gcggcgccca cgagcgcgcc gatgtctacg ggaccgaacc tccgctggcc gggggcaccg
     1201 cttcgccggc gcgcaagatc gagcatctgt tcgaccatgg tagacatgcg cccgatctct
     1261 tccagcgagc tcgtgagggc cctacgggcc tccgacacgc cgaaggtccg ggaccgcagg
     1321 gccacctccg cctccgtccg catcacggac aggggcgttc gaaggtcatg ggccgcgttc
     1381 gcgaagaaaa gctgttcgtt ctccaccgac cgcttgatgg ggcgaagggt caaggctgcc
     1441 aggaggtagc tgagcgcccc gccgaccacg atggtcagac cgcccgcgat caggacggtc
     1501 cgcccgagct cctccagata gtcctccacg ttgggggaaa gctcggccag accctcgcgc
     1561 tcgcggacca tctctcgacg cacccgctcg gagcgggcta cctctcccgc gtgcagggag
     1621 tacagggaga agctcaacac aacgacgatg accgtgatca gggcgagata gagaagcgtg
     1681 agcttgatcc gagcggtcgt gaacgggtca gcccttgagg cggtatcccg cgccccgaac
     1741 ggtctcaata gggtcatgct tccccgcctc ctcgagcttc cgcctcaggt tccggatatg
     1801 gacctccacg acgttgcttc ctccatcgaa ctcctggtcc cagaggctgt tggtgatctg
     1861 gtcccgggag agcacgtgcc gggggagtcg gaggaagagc tcgagcaggc ggaactcctt
     1921 ggaggtcagc accaccgcct tgcccaggac cttcacctcg tgggtattgg agtcgagctc
     1981 gatgtcgccc acggagagca cgacatccgg cgctcttcgg ggccggcgca ggagcgcccg
     2041 aagccgggcc gccagctcct cgaacgcgaa tggcttggtc aggtagtcat ccgcgccggc
     2101 atccaacccg cccacccgat cccgcacccc gtctcgggcg gtcagcatga ggacgggggc
     2161 ctgttctcca tgcgctctca tcgcccgaca aacgctcagt ccgtccttgc gcgggagcat
     2221 gaggtccagg atcaccgcgt cgtatccatt cttcagggaa tccaccagca gcaggccttc
     2281 ttccccgtcc agggccacat ccacggcgta gccctcctgc tccaggccgg tcttcaccga
     2341 ggcggcaagc ttctcgttgt cttcgatgac gaggatccgc acggcggggc ctaggcgaac
     2401 cactcccgac cggtctcaga ggagatgccg cgcgtcttca cgagctcctg ttgcgcctcg
     2461 gactccatga cgtatgccgc cgcggcgatc tggcgcgaga taggggtagg ctgcgcggtg
     2521 gcaacgatct gcagaagctg ctcggccttg aggatggtca ggcgcggggc gctccccacc
     2581 gcgtcggcag gctgcgctcg aggcgacggc gcggcggctg ccgtggtccg agaaccgccg
     2641 acggccggcc gatccagcac ccgcccagag ccttggacca ggatgtccgc gctatcgagt
     2701 ggatccatgg gcttccaccg ttacctgcaa tgtaccctca ggcagggttt cgcgcaaggc
     2761 gagacccgca gacccccgca caccgtgatc cgcatggtcc gccgcctggt ggggcggtga
     2821 ggttgatggc ttcgcaggcc gccgtctata atgccttacc ccacgtagca caagcagcct
     2881 gaagggttca ttcgaacgaa ggaggcaggg aatggcagtc gcagaaggac tcggccgaaa
     2941 ggtccggacc gtccgtgaat cgaggaagct cagccgccag gagatggccg aacgcgccgc
     3001 actgccggcc gagctggtcg cccagatcga gggggacgag ctggtcccct ccctgagcct
     3061 gctcctccgc atcgcccggg tgctcggggt gcgcctgggg acattcctgg acgaccagga
     3121 gaacgtgggg gcggtggtga cccgatccgg tgagctgccg cgcgtgacgc ggttctccag
     3181 ccggatccac gcgggcccca gcagcgacct ggacttccac tcgctggccg tcaacaagag
     3241 cgggcggcac atggagccat tcctcatcga tatccatccg tcttccgccg aatcggtcca
     3301 gccgtcgagc cacgaggggg aagagttcat ccacgtgttc gagggagagg tggaggtgct
     3361 ctacggcaag gacacgtatc gcctgggtgt gggggactcc atctactacg actccgtagt
     3421 gtcccatcac gtgcactgca gcggcggcga cacggcccgc atcctggccg tggtgtatgc
     3481 gccgctctga gcgaggaggg accgcatgca gtttctcgac atgacgctcg gccagatgct
     3541 ggagaagcag gcggccgctt acccggaccg ggacttcatg gtctatgcgg accgcgacct
     3601 gcgcttcacc taccgccagt tcaaccaacg ggtcgaccgg ctggccaagg gactgcttgc
     3661 catcggcctc cacaagggcg accacctcgg gatctgggcc acgaacgttc ccgactggct
     3721 caccgtgctg ttcgccaccg ccaagatcgg cgtggtgctg gtgaccgtca acacggccta
     3781 tcggacccat gagctggagt acctgatcca gcagtcggac atggacaccc tctgcctgat
     3841 caacggcttt cgggattcgg attacgtggc catggtcaac gagctggtac cggagctcaa
     3901 gacgtgccag cgcggacacc tgtgcagcga acggttccct cggctgcgga acgtgatctt
     3961 tctgggaccc gagaagcacc gcgggatgtt cagcacgccg gaagtcctgc tcctgggcga
     4021 gcaggtgcgg gacgaagagc ttgagagagc ctcctcgggc ctgagcagcg ccgaggtgat
     4081 caacatgcag tacacctcgg ggaccaccgg tttccccaag ggcgtgatgc tcacgcacaa
     4141 caacatcctg aacaacggct tccagatcgg agaacggcag cgcttcacgg aacaggaccg
     4201 cgtatgcctg cccgtgccgt tgttccactg cttcgggctc gtgctcggca tcatggccat
     4261 ctacacccac ggcgccgcgg cggtcgtcct ggaggccttc gacccggtca tggcgctggc
     4321 ggccgtccag aaggagaagt gcaccgcgct gtacggcgtc ccgaccatgt tcatcgccga
     4381 gctcaaccac ccgatgttct cgatgttcga caccagctcg ctgcgcaccg ggatcatggc
     4441 gggctctccg tgcccgatcg agaccatgaa gcaggtgatc gaaacgatgc acgccggcga
     4501 gatcacgatc tgctacggcc tcacggaggg atcccccgtg atgacgcaga cttccacgga
     4561 tgactccgtg gacctccgag tctccaccgt gggccgggcg ctgcccagga tcgaggtgcg
     4621 aatcgtggac ccgt
//
LOCUS       DASRZS010000134         8693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3919683, whole genome shotgun sequence.
ACCESSION   DASRZS010000134 DASRZS010000000
VERSION     DASRZS010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8693
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3919683"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFH83_03350"
     CDS             complement(<1..574)
                     /locus_tag="VFH83_03350"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6485429.1"
                     /translation="MAVKSRLLRSLIPWFVSRGLTGRASDLKRIIRVARRIPTTVAAR
                     RFFDGLEYSLEHHEAMGELFLRIGRQLSPRGRSRISTNLIYHQFIAGYPIRRKHIEEG
                     NWVPGFFVMSPTMRCNLTCRGCYSAIYPRQEELSFEEMDRILTEAEDLGIYFIVISGG
                     EPYLLSDRLLPLFRKHRKQYFLTYTNATLLD"
     gene            complement(689..2557)
                     /locus_tag="VFH83_03355"
     CDS             complement(689..2557)
                     /locus_tag="VFH83_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET6485430.1"
                     /translation="MIHIGDGSFRDDDGRALMLRGVNVAGSSKVPRSPNGATHLREGF
                     LDHRHVSFVGRPFPLAEAEEHLARLSGWGLTVLRLVVTWEAIEHAGPGIYDEEYLDYL
                     RDLVTLADRHGLAVLIDPHQDMWSRFSGGDGAPGWTLEAIGFDLEALDETGAAFTHQA
                     RGDPLPAMIWASNSGKLAASTMFTLFLGGTDFAPKTRVNNEPVQEYLQRHYVQAIMTL
                     VRRLKGVKGVLGYEAMNEPMPGYIGWKDLRRPGGLITVGDCPSPFQGMALGAGIPQSV
                     DVWRLGSLSLVKSAERLLNPSRRSAWKSGFDCPWRENGVWRASPDGHPELLRPDHFTQ
                     VDGKTVGFNDYWEPFVRRFGAALRVTDPDAKLFIEGSPGQPSPSLEAASGQAVAFAPH
                     WYDDLLLAKKRYLSFLGLDSRTRTVVLGRRAVRRCFADQLASYKRHGSEWGVPSLLGE
                     FGIPFDLNGARAYRTGSFAVQARAMDRSFRAVEDNLLSCTLWNYTPDNTNARGDLWNG
                     EDFSIYSRDQKTDTAPDAGGRALEAVVRPYPRATAGKPLRLRFEPRRRRFFFEFRGHS
                     VSELPTEIFVPRLQYPSGYRVSVSDGDYLVVPDRQALLYTPGGGRSTHWIRILPKR"
     gene            2662..4167
                     /locus_tag="VFH83_03360"
     CDS             2662..4167
                     /locus_tag="VFH83_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6485431.1"
                     /translation="MKEKVIIIGAGVYGLSLGSYLAMNDLDVEILEMHNAAGGVCTSW
                     KRGGYTFDGCIHWLMGSGPGSGLHELWKELHAVQGRRFVEWEEYATVRTRGGERFTVY
                     TDPDRLEAEMLRIGPQDARTIRELCDAIARMSRVDIPVGMETMSLSRKISAGLGALPM
                     LGAMRRWGSVTFQSFCARLKSPALAEALRCLYGAEDDMPDFPVMGLIMMLAFMHKKTA
                     GYAIGGSREFAHAIEQRFCELGGTIRYGTRVDRIIVEGNRAVGVASGMHEHRADVVVS
                     CADGHATLYGMLGGRYLSPRIRTAYGTYQTFPAPIYTGIGIARDLRDTPALLLFPLRK
                     EMVLEDGALRVNKLSVRLFSFDPTMAPAGKTAATVMITTRNHEYWTRLRAVDPGRYRQ
                     EKSRIGELIVDALDQEIGDIQRHVEVVDVSTPATWIRYTGNWKGSFEGFLPTRKTMMQ
                     NLGFTLPGLRGFYMNGHWVSVGGGLPPAAMNGRTLARMICRDLGKEFEVVA"
     gene            complement(4180..6900)
                     /gene="fdhF"
                     /locus_tag="VFH83_03365"
     CDS             complement(4180..6900)
                     /gene="fdhF"
                     /locus_tag="VFH83_03365"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HET6485432.1"
                     /translation="MPMVKLSIDGREVHAMAGTLLLPAAREAGIQIPSLCWHRKLTPT
                     GACRLCLVKIEGTRGLVTSCSTIVKEGMRVTAFDDELETTRRFLLDYLLAEVRCGSDG
                     SFRDEFAELVDRYGLSDPSARTFPVLTSVPRPPLDASSPVLTFDPSRCILCFRCVKAC
                     AEVQGKGVLSVVERGERSVIAAGSGTWSSSECDGCGECIQLCPTGAIVEKPHRSQIDL
                     SRTRTERTTCPYCGVGCQIDLAVQDGRILRADGAEEVPPNDGRLCVKGRFGYDFVHSP
                     DRLTHPLIKENGGFREASWDEALDRAADGLSAIKRRHGADALAGYSSAKCTNEENYLF
                     QKLVRVAFGTNNVDYCTRLCHASTVTGMLRAIGDGAGSNSIVDFETTDCLLVIGNNII
                     ETHPVTATYVKRGRARGQRIVVVDPKETPLVRYADVWLQPRLGTDIALLNGMIRAVIQ
                     GGWVDRDFITRRVSGGMEAFGELERLTEKYTPAYTEAITGVSPELLEEAARLYATSAT
                     AMIATGMGMSQQTTGTHNVFCLINLMLITGKIGRERCGMDPPRGQNNVQGATDVGASP
                     LYLPGYLSISDEGNRRKVAEVWGVPLEEIPAERGLTTVEIVKAAADKKIRGMLIMGEN
                     PVLTDPNSAHAEEALRSLEFLVVQDIFLTETARLADVVLPAACFAEKDGTFANSDRRV
                     LRVRKAVEPPGEAREDLVILQEIARRMGRPIGGYHDASQVFDEIARVAPIMAGIDYSR
                     IESRGIQWPCPTRDHPGTATLFLERFNTPDGLARLNPVDYEPQSERSDDAYPFLLNTG
                     RILYQYHSCTMSRRSPPLVDYANDAYLLMHPCDADRLGLGDGEMVRITSRRGSIEAPL
                     RRSEEVAEGELFMPFHFGEAPVNRLTRDELDPFSRIPPFKLSACRVERRA"
     gene            complement(6914..8620)
                     /locus_tag="VFH83_03370"
     CDS             complement(6914..8620)
                     /locus_tag="VFH83_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET6485433.1"
                     /translation="MDPRDRLLSVLHGVQDASQRNYLPPEQLDEIRRRFSITRAELRG
                     VASYYSLFSLRPRGRHVVRVCVSPICRMIGSLDLLDLLRAELGVDVGQTTADGLFTLE
                     ETQCLGRCAGAPVLMVDGRIYEQRTAEGVRRLLEASRRGAPSAASRAGSARKPAAGER
                     RIALRYVGAVPPLDLDAYRAAGGYTALRKALTQEPASVVAELTRAILRGRGGAGFPAG
                     VKSKAASDTAAVCERYVVCNADEGEPGTFKDRIILEGEPHLLLEGMAICGFAIGAPKG
                     YIYVRGEYGLSIQRLKRAIQDAYAAGLLGKSVLGSGIPFDIEVKVGAGSYLCGEELTL
                     LESLEGKRGYPRIKPPFPAESGLWGMPTLVNNVETLANVPFIVTEGASAYLSIGTPSS
                     PGTKIFCLSGDVNRPGHVEVEMGITLRSLIDDFGGGVKGGGPPAAVLLGGANGAFVSA
                     SMLDLPMDFDALRKAGATLGSGAVIVLGPGRSIPVMLSAILDFFRHESCGKCIPCRVG
                     TARLAERARSLEGLAPEARRAALREMVREAEMMEKTSLCPLGQSPVLPLRSAMAHLAD
                     GI"
BASE COUNT         1483 a         2889 c         2910 g         1411 t
ORIGIN      
        1 cgtccaggag ggtggcgttg gtgtacgtga ggaagtactg ctttcggtgc ttgcggaaga
       61 gcggcagaag acggtccgac aggagatagg gctctccgcc ggagatcacg atgaagtaga
      121 tcccgagatc ctccgcctcc gtcaggatcc ggtccatctc ctcgaagctg agctcctcct
      181 gcctggggta gatcgcggag tagcatccgc ggcaggtcag gttgcatcgc atggtcggac
      241 tcatgacgaa gaacccgggg acccagttcc cttcttcgat gtgcttcctg cgtatggggt
      301 agccggcgat gaactggtga tagatgaggt tggtcgagat ccgggagcgg cctcttggag
      361 agagctgacg tccgatcctg aggaaaagct cccccatcgc ctcgtggtgc tccagcgagt
      421 attccagccc atcgaagaag cgtcgagccg ccacggtggt gggaatgcgg cgggccactc
      481 gaatgatgcg cttgaggtcc gaggcccttc ctgtcagccc gcgtgagacg aaccacggga
      541 tgagcgaccg aaggagacga cttttgactg ccatagggac cacctcaccg tcagcggaga
      601 acggtctccc ttcagcacgg atcgcctgcg acgagatcag tgtacgcccg acgccacggg
      661 tgtcaagaat ccctccgcca gcgagcccct atcgcttcgg caggatgcgg atccaatggg
      721 tcgagcggcc cccaccgggg gtgtacaaca gggcctgccg gtcgggcacc accaggtagt
      781 ctccgtcact caccgacacg cggtagccgc tcgggtattg caggcgtggc acgaagatct
      841 ccgtcggcag ctcggagacg gaatggccgc ggaactcgaa gaagaacctg cgtcgcctcg
      901 gctcgaagcg gaggcggagc ggctttcccg cagtcgctcg aggatagggt cggaccaccg
      961 cctccagggc tcggcccccg gcatccggtg cagtgtcggt cttctggtcc cggctgtaga
     1021 tcgagaagtc ttccccgttc cacagatcgc ctcgggcgtt ggtgttgtct ggcgtgtagt
     1081 tccagagcgt gcacgagagg aggttgtcct cgacggctcg aaagcttcgg tccatggccc
     1141 gtgcctgaac ggcgaagctc cccgtgcgat aggcgcgcgc gccgttcaga tcgaaaggga
     1201 tgccgaactc ccccagcagg ctgggcacac cccactcgga cccgtgccgc ttgtagctgg
     1261 caagctggtc cgcgaagcat cgccggacgg cccggcggcc caagaccacg gtccgcgtcc
     1321 gagagtccaa ccccaggaag gacaggtacc gcttcttggc cagcaggaga tcgtcgtacc
     1381 agtggggggc aaaggcgact gcctgccccg aggcagcttc cagggaagga gacggttggc
     1441 ccggtgatcc ctcgatgaag agcttcgcgt ccggatccgt caccctgagg gcggcgccga
     1501 acctgcgcac gaacggttcc cagtagtcgt tgaacccgac ggtcttgcca tccacctggg
     1561 tgaagtggtc cggccgcagg agctcgggat ggccgtcagg agaagcccgc cagaccccgt
     1621 tctccctcca cgggcagtcg aacccgctct tccacgcgct ccgcctggac gggttgagca
     1681 ggcgctccgc ggacttcacc aggctcaggc tgccaaggcg ccagacgtcg acgctctgag
     1741 gaatcccggc tcccaaggcc atgccctgaa agggagaagg gcaatctcct acggtgatga
     1801 gccctcccgg ccgcctcagg tccttccacc cgatgtagcc cggcatgggc tcgttcatgg
     1861 cctcgtaccc caggacgcct ttcacgccct tcaatcgccg cacgagcgtc atgatggcct
     1921 gcacgtaatg gcgctgcaga tactcctgga ccggttcgtt gttcacccgg gtcttggggg
     1981 caaagtcggt cccgcccagg aacagggtga acatcgtgga ggccgccagc ttgccgctgt
     2041 tggatgccca gatcatggcc gggagcggat ccccgcgcgc ctggtgggtg aacgccgctc
     2101 cggtctcgtc cagcgcctcc aggtcgaacc cgatcgcttc cagggtccag ccgggcgcgc
     2161 catctccgcc gctgaaccgg ctccacatgt cctggtgcgg atcgatcagc accgccagac
     2221 catgccggtc cgccagcgtc acgagatcgc ggaggtagtc gaggtactcc tcgtcgtaga
     2281 tccccggccc cgcgtgctcg atggcctccc acgtcacaac cagccgcagg acggtgagcc
     2341 cccagcccga aagccgggca agatgctcct ccgcctccgc caggggaaac ggacgaccca
     2401 cgaaagagac atgccggtga tcgaggaacc cttcccgaag gtgcgtcgcg ccgttcggcg
     2461 atcttggcac cttgctggac cccgccacgt tcacccctcg aagcatcaag gcgcgcccgt
     2521 catcgtcccg gaacgatccg tcgccaatgt ggatcatggc ttccgttcta ggtctcctcg
     2581 gcctccgggt caagggaccc ggcgtggaga cacggaagca cgcccgcggg gttgaaaggg
     2641 gagatccacc aggaggagcg agtgaaagag aaagtcatca tcatcggcgc cggggtgtat
     2701 gggctctctc tgggaagcta cctggccatg aacgatctgg acgtcgagat cctggagatg
     2761 cacaacgccg cgggcggcgt ctgcacctcc tggaagcggg gcgggtacac gttcgacggc
     2821 tgcatccact ggctgatggg ctcgggtccg ggctccgggc tgcacgagct gtggaaggag
     2881 ctgcatgcgg tccagggacg acggttcgtg gaatgggaag agtacgcgac cgttcgcacc
     2941 cgaggcgggg agcgcttcac ggtttacacg gaccccgacc ggctggaagc cgagatgctg
     3001 cggataggcc cgcaggatgc caggaccatc cgggagctct gtgatgccat agcccggatg
     3061 tccagagtgg acatccccgt gggcatggag accatgagcc tgtcccgaaa gatctccgcg
     3121 ggactcggag cgctgcccat gctcggggcg atgcggcggt ggggatcggt caccttccag
     3181 tccttctgcg cgagactgaa gagccctgcg ctggcagagg cgctccgctg cctctatgga
     3241 gcggaagacg acatgccgga ctttccggtc atgggcctga tcatgatgct cgccttcatg
     3301 cacaagaaga ccgccggcta cgcgatcggc gggtctcggg agttcgccca cgccatcgag
     3361 cagcggttct gcgagctggg cgggaccatc cgctacggca cccgcgtgga ccggatcatc
     3421 gtggagggca accgcgcggt gggcgtggcc agcgggatgc atgagcatcg ggcggacgtc
     3481 gtcgtctcct gcgccgacgg tcacgcgacg ctctatggga tgctgggggg acgctacctc
     3541 agcccccgga tccgcaccgc ctatggcacc taccagacct tcccggcgcc catctacacg
     3601 gggatcggga ttgcgcggga tctgcgcgac acgccggcct tgctcctgtt ccctctgcgc
     3661 aaggagatgg tcctggagga cggtgcgctt cgcgtcaaca agctgtccgt tcggctcttc
     3721 agtttcgatc ccaccatggc gcccgccggc aagacggccg ccaccgtgat gatcaccacc
     3781 cgcaaccatg agtactggac gcgcctgcgg gcggtcgacc cgggccgcta ccggcaggag
     3841 aagagccgca tcggggagct catcgtggac gcgctggacc aggagatcgg ggacatccag
     3901 cgtcacgtgg aggttgtgga cgtctccacg ccggcaacct ggatccgcta caccggcaac
     3961 tggaagggaa gcttcgaggg cttcctcccg actcgcaaga ccatgatgca gaacctgggc
     4021 ttcaccctgc ccggccttcg cgggttctac atgaacgggc actgggtgtc cgtgggcggc
     4081 gggctgccgc cggccgccat gaacggaagg accctggcca ggatgatctg cagggacctg
     4141 gggaaggagt tcgaggtggt ggcgtagggc ggctcgccct tacgcccgcc gttccacccg
     4201 gcacgccgag agcttgaacg gtgggatccg cgagaacgga tccagctcgt cccgcgtgag
     4261 acggttgacg ggagcctccc cgaaatggaa gggcatgaag agctcccctt ccgccacttc
     4321 ctcactgcgg cggagggggg cctcgatgct cccccgccgg gaggtgatcc gcaccatctc
     4381 gccgtctccc aaccccaacc ggtccgcgtc gcaaggatgc atgagcaggt aggcgtcgtt
     4441 ggcgtagtcc accagaggcg ggctgcggcg ggacatcgtg caggaatgat actggtagag
     4501 gatccggccc gtgttgagga ggaacgggta ggcatcgtct gatcgctcgc tctgcggctc
     4561 gtagtctacg gggttgaggc gggccagccc atccggcgtg ttgaaccgct ccaggaagag
     4621 ggtggcggtt cccggatggt cccgcgtagg gcacggccac tggatcccgc gggactcgat
     4681 ccgcgagtag tcgataccgg ccatgatggg ggccacccgg gcaatctcgt cgaacacctg
     4741 ggaggcgtcg tgatacccgc cgatgggtct gcccatgcgg cgcgcgatct cctggaggat
     4801 gacgaggtct tcccgtgcct cccccggcgg ctccacggcc tttcggacgc gcagcacccg
     4861 acggtcgctg ttggcgaagg tcccgtcctt ctccgcgaag caggcggccg gcagcaccac
     4921 gtccgcgagc ctcgccgtct ccgtcaggaa gatgtcctgc accaccagga actccaggga
     4981 ccgcagcgcc tcctccgcgt gcgcggagtt cgggtcggtg aggaccgggt tctcccccat
     5041 gatgagcatc ccgcggatct tcttgtccgc cgctgccttc acgatctcca cggtcgtgag
     5101 gccccgttcc gccggtatct cttcgagtgg aaccccccag acttccgcga ccttccggcg
     5161 gtttccctcg tcggagatgg acaggtagcc gggcagatag agcggagatg ccccgacgtc
     5221 cgtggcgccc tggacgttgt tctgaccccg cgggggatcc atgccgcaac gctcccgccc
     5281 gatcttgccc gtgatcagca tcaggttgat gagacagaag acgttgtggg tgcccgtggt
     5341 ctgctggctc atgcccattc ccgtcgcgat catggcggtg gcggaggtgg cgtagaggcg
     5401 cgccgcctct tccagcagct cgggggagac gccggtaatg gcctccgtat aggctggcgt
     5461 gtacttctcc gtgagccgct ccagctcgcc gaacgcctcc atcccgcccg agacgcgtcg
     5521 ggtgatgaag tcccggtcga cccagccgcc ctggatcacc gcgcggatca tgccgttcag
     5581 aagggcgatg tcggtaccca gccgaggctg gagccagacg tccgcgtagc ggaccagggg
     5641 cgtttccttc ggatccacga caacgatccg ctgcccgcgc gcccgtcccc gcttcacgta
     5701 ggtggcggtc acgggatggg tctcgatgat gttgttcccg atgaccagga ggcagtccgt
     5761 ggtctcgaaa tccacgatgg agttgcttcc ggctccgtcg ccgatggccc ggagcatccc
     5821 ggtgacggtc gaggcgtggc agagccgggt gcagtagtcg acgttgttgg tcccgaaggc
     5881 cacgcgcacc agcttctgga agaggtagtt ctcctcgttg gtgcacttcg cggaggagta
     5941 gccggcaagc gcgtccgcgc cgtgccgcct cttgatggcg gaaagaccgt ccgcggcccg
     6001 gtccagggcc tcgtcccagg atgcctcgcg gaagccgccg ttctccttga tgaggggatg
     6061 ggtcaaccga tccgggctgt ggacgaagtc atacccgaac cggcctttca cgcacagccg
     6121 cccgtcgttc ggtgggacct cctccgcgcc atccgccctc aggattctgc cgtcctggac
     6181 ggccaggtcg atctggcatc ccacgccgca gtacgggcag gtggtccgct ccgtgcgggt
     6241 ccgggagaga tcgatctggg aacggtgcgg cttctccacg atggcgcccg tggggcagag
     6301 ctggatgcac tccccacagc catcacactc ggaggaagac catgtcccgc tgccggcggc
     6361 gatcaccgaa cgttcaccgc gctcgacgac ggagagcacg cctttgcctt gcacctccgc
     6421 gcacgccttc acgcatcgga agcaaaggat gcagcgggaa ggatcgaagg tcagtaccgg
     6481 cgaagaggcg tccagcggcg gacgcgggac cgaggtgaga acggggaacg tgcgcgcgga
     6541 cggatccgaa aggccatagc gatcgaccag ctcggcaaac tcgtctcgga aggagccgtc
     6601 ggagccgcag cggacctccg ccaggaggta gtccaggagg aaccggcgcg tggtctccag
     6661 ctcgtcgtcg aacgcggtca cccgcatgcc ctccttgacg atcgtggaac aggaggtgac
     6721 gaggccccgc gttccttcga tcttcaccag gcacagccgg caggcgccgg tgggcgtcag
     6781 cttgcggtgc caacagaggc tggggatctg gatccccgct tcccgcgcgg ccggcaggag
     6841 gagggtcccg gccatggcgt gaacctcccg gccatcgatc gaaagcttga ccatcggcat
     6901 cgtcgagtct cctctagatc ccgtcggcga gatgggccat ggcgctgcgc agaggaagca
     6961 cgggcgattg cccgagcgga cacaaggacg tcttctccat catctccgcc tcacggacca
     7021 tctctcgaag cgcggcccga cgggcctccg gagccaaccc ttccagcgac cgagcgcgct
     7081 cggcaagcct ggccgtcccc acccgacagg ggatgcactt cccgcaggac tcgtgacgga
     7141 agaagtccag gatggcggag agcatcaccg ggatggagcg acccggcccc aggacgatga
     7201 cggctccgga cccgagcgtg gctccggcct tgcgcagcgc atcgaagtcc atgggcaggt
     7261 ccagcatgga ggcggacacg aaggctccgt tcgcgccccc gagcaggacc gcggctggag
     7321 gacccccgcc tttcacgccg ccgccgaagt cgtcgatcag gcttcgcagg gttatcccca
     7381 tctccacttc cacgtgtccc ggacgattca cgtctccgct caagcagaag atcttggtcc
     7441 cgggggacga aggggtcccg atggaaaggt aggcgctcgc gccctccgtc acgatgaacg
     7501 ggacgtttgc cagcgtctcg acgttgttga cgagggtggg catgccccag agcccggact
     7561 ccgcgggaaa cggcggcttg atgcgtgggt agccgcgctt gccctccagg gattccagaa
     7621 gggtcagctc ctctccgcag aggtaggagc cggccccgac cttcacctcg atgtcgaacg
     7681 gtatcccgct gccaaggacg ctcttgccca ggagcccggc ggcataggcg tcctggatcg
     7741 cgcgcttcag cctctggatg gacaggccat attcgccgcg cacgtagatg tagcccttgg
     7801 gagcgcctat ggcgaaaccg cagatcgcca tcccttccaa caggaggtgc ggctcccctt
     7861 ccaggatgat gcggtccttg aacgtgccgg gctccccctc gtccgcgttg cacaccacgt
     7921 agcgctcgca gacggccgcc gtgtccgatg cggccttgct tttcacgccc gccgggaagc
     7981 ccgctccgcc gcggccccgc aggatggcgc gggtcagctc ggccacgacg gaggcgggct
     8041 cctgggtcaa ggccttgcgg agcgccgtgt agcccccagc cgcgcggtag gcgtcgaggt
     8101 ccagcggcgg cacggcgccc acgtaacgaa gcgcgatgcg ccgctccccc gccgccggct
     8161 ttcgggcgct gcccgcgcgg ctggccgcgc tcggcgcgcc ccggcgggag gcctccagca
     8221 gacggcggac gccctccgcg gtccgctgct cgtagatccg gccgtccacc atcaggacgg
     8281 gcgcgcctgc gcaccggccc aggcactggg tctcctccag ggtgaaaaga ccgtccgccg
     8341 tggtctgccc cacgtccacg cccagctccg cgcgaagcag gtccaggagg tcgagcgaac
     8401 cgatcatgcg gcaaatggga gacacacaga cccggaccac gtgcctgccg cgcggtcgaa
     8461 ggctgaagag ggagtagtag gaggccacgc cacggagctc cgccctggtg atcgagaatc
     8521 ggcgtcggat ctcgtccagt tgttccggcg ggaggtagtt gcgctgggag gcgtcctgga
     8581 ctccatggag gaccgacagg agacggtctc gcggatccat agggaccttc agtatagacc
     8641 gccccccggt cgatgtcaaa gataatcccg gacaggaaaa caccgacacc gat
//
LOCUS       DASRZS010000135         3853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1997961, whole genome shotgun sequence.
ACCESSION   DASRZS010000135 DASRZS010000000
VERSION     DASRZS010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997961"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFH83_03375"
     CDS             <1..909
                     /locus_tag="VFH83_03375"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6485434.1"
                     /translation="ADTIQSHNASREAMLNAYGRFVPHELIRLLNKGTIVDVQLGDQT
                     QREMTVLFSDIIAFTTLSESMTPYENFNFLNSYLRRIGPEIRANNGFIDKYIGDAIMA
                     LFPERPDDAMAAAVGMQEKLREYNVHRAKSGYQPISVGVGVHVGKVMMGTLGEQERMD
                     GSVISDAVNLCSRIQELTRVYATSIITTGATLKSLREPDRFKCRFIDRVRVRGRRETV
                     VLFEVLDGELQEQRELKLSYRNELAHALKLYYGKEFEEALGIISRLIPVNPKDEVLRI
                     YRKRCELLVNLGTPEGWVGVEEVSAR"
     gene            1284..1769
                     /locus_tag="VFH83_03380"
     CDS             1284..1769
                     /locus_tag="VFH83_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET6485435.1"
                     /translation="METATEKRKIGDVIARFPPQREHLISILQEVQGEYGYLSRDSIA
                     TISSYLNLPSSKIFGVATFYNQFRLYAPGRIQVAICRGTACHVKGSLNLLDNCRQLLG
                     IEVGQTDKEGLFSLETVACLGCCSIAPAMMVNGRFFGRLDKKTLETLIDRFRSTGRVE
                     E"
     gene            1766..3583
                     /locus_tag="VFH83_03385"
     CDS             1766..3583
                     /locus_tag="VFH83_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HET6485436.1"
                     /translation="MSQVFIGMGTCGLATGAGQVKTAVEKWAAARGVKMAITATGCIG
                     YCKAEPIMDVVTEGGHRVSYGNVTPDVAGFILDEVFVHKNLKLDGILGQFVNGAGALP
                     DIPRIEDHPYFKKQVKYVLRNCGIIDPESISDYRAHGGFKGLERALGMTQKQVADEML
                     ASGLRGRGGSGFPAGKKWDLARVQPGEKKYVICNADEGDPGAFMDRSILEGDTYSVLE
                     GMMIAGYAIGADEGIIYCRAEYPLAIERIEKAITVLKQEHLLEAPQGKFRFALRVKKG
                     AGAFVCGEETALMASVEGRRGMPRPRPPFPAVSGLFQKPTIVNNVETMANASHIMGEG
                     AESFAAMGTATSKGTKVFALTGKVANAGLVEVPMGITLREIVYDIGGGIPGGKAFKAV
                     QIGGPSGGCIPAEHLDIKIDYESLKSVGAIMGSGGLVVMDEDTCMVDMANYFLQFITS
                     ESCGKCIPCREGTQRLHEILESIVSPYSKMASEEENLLRFNRVLNMESLAQVIKDTSL
                     CGLGQTAPNPVLSTLRFFRSEYEAHIYDRRCPAHACKGLLTYTIDTNACTGCTLCAKK
                     CPTNAIVGEKKKAHYILEEKCIRCGTCKDVCRFNAVNVS"
     gene            3620..>3853
                     /locus_tag="VFH83_03390"
     CDS             3620..>3853
                     /locus_tag="VFH83_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET6485437.1"
                     /translation="MVTITINNRKLQVKPGISILDAAKEAGERIPTLCHIQELFPSGA
                     CRMCVVEVKGKPNLTPSCAFPAEEGMEIQTRSPR"
BASE COUNT          763 a         1214 c         1241 g          635 t
ORIGIN      
        1 gcggatacca tccagagcca taacgccagc cgcgaagcca tgctcaacgc ctacgggcgg
       61 ttcgtcccgc acgagctcat ccgtctgctc aacaagggca ccattgtgga cgtgcagttg
      121 ggggaccaga cccagcggga gatgaccgtc ctcttctccg acatcatcgc cttcacgacg
      181 ctctccgaga gcatgacgcc gtacgagaac ttcaacttcc tcaactccta cctccgacgc
      241 atcggtccgg agatccgggc caacaacggg ttcatcgaca agtacatcgg ggacgccatc
      301 atggccctct tccccgagag accggatgac gcgatggccg cggcggtcgg aatgcaggag
      361 aagctgaggg agtacaacgt ccaccgggcc aagagcggct accagcccat ctccgtcggc
      421 gtgggggtcc atgtgggcaa ggtgatgatg gggacgctgg gggagcagga gcggatggac
      481 ggctcggtga tctccgacgc cgtgaacctc tgctcccgga tccaggagct gacccgcgtg
      541 tacgcgacgt cgatcatcac caccggagcg accctgaagt ccctgcgcga gccggatcgg
      601 ttcaagtgcc gcttcatcga ccgcgtccgc gtccgaggaa gacgggagac cgtggtgctc
      661 ttcgaggtcc tggacggcga gctccaggag cagcgggagc tgaagctctc ctatcgcaac
      721 gagctggctc acgccctgaa gctctactac ggcaaggagt tcgaggaggc gctggggatc
      781 atcagccggc tcataccggt gaacccgaag gacgaggtgc tccggatcta ccgcaagcga
      841 tgcgagctcc tggtgaacct cggcacgccg gagggctggg tcggggtgga ggaagtctcg
      901 gcgcggtagc caaaccggcc gcccaccccg ttctgggtat cggccctcgg tcatccccgg
      961 gcctgtagcc gcccgtgatg gcgccgtccg ccggccgcct cctgatgagg cgctgaccaa
     1021 ctggcaaagc cagcgatcga gactcagtgc cctcgaaaac caatcgccct caatgggttc
     1081 gatttccggg aagcatattg acttttccat cacgagatgg tatagattat attgtgaatt
     1141 agatcacgat taaaacaggc tcaggatctc tcaagtagcc attcactaaa gtcacgatag
     1201 agccaggggc agggcaaggc ttcagtcacc agcctgacca cggctcgcaa taggacgcaa
     1261 ctcgaaccat gggaggcgct ttgatggaaa cagcaacgga gaaacgcaag ataggggacg
     1321 tgatcgccag gtttccgccc cagcgcgagc acctgatctc gatcctgcag gaagtccagg
     1381 gcgagtatgg ctacctgtcc cgggacagca tcgccacgat ctcctcttac ctcaaccttc
     1441 cgtcctccaa gatcttcggc gtcgccactt tctacaacca gttccggttg tacgcgccgg
     1501 ggcggatcca ggtcgctatc tgccgcggaa cggcctgcca cgtaaagggc tccctgaacc
     1561 tcctcgacaa ctgccgacag ctcctgggga tcgaggtggg ccagacggac aaggagggtc
     1621 tgttctccct ggagacggtg gcctgccttg ggtgctgctc gattgccccg gccatgatgg
     1681 tcaacggcag gttcttcggc cggctggaca agaagacctt ggagaccttg atcgatcggt
     1741 tccgcagcac ggggagggtc gaggaatgag ccaggttttc atcgggatgg ggacctgcgg
     1801 gctggcgacc ggcgcgggcc aggtgaagac cgcggtggag aagtgggcag cggctcgcgg
     1861 cgtgaagatg gccatcacgg cgaccggctg catcggctac tgcaaggcgg agccgatcat
     1921 ggacgtggtc accgagggcg gccatcgcgt ttcctacggc aacgtgacgc cggacgtggc
     1981 tggcttcatc ctggacgagg tcttcgtcca caagaacctc aagctggacg gcatcctggg
     2041 ccagttcgtg aacggggccg gggcgcttcc tgacatcccc cggatcgaag accaccccta
     2101 cttcaagaag caggtcaagt acgtgcttcg caactgcggg atcatcgatc ctgagtccat
     2161 ctccgactat cgcgcccacg gcggcttcaa gggactggaa cgcgctctgg gcatgaccca
     2221 gaagcaggtg gccgatgaga tgctggcctc cggccttcgc ggccgaggcg gaagcggttt
     2281 tcccgccggc aagaagtggg acctggcccg ggtccagccg ggcgagaaga agtacgtgat
     2341 ctgcaacgcg gacgagggag accccggcgc cttcatggac cggtccatcc tggagggaga
     2401 cacctactct gtcctggagg ggatgatgat cgccggctac gcgatcggcg cggacgaggg
     2461 gatcatctac tgccgggccg agtacccgct ggccatcgag aggatcgaga aggccatcac
     2521 ggtgctcaag caggagcatc tgctcgaggc gccccagggc aagttccgct tcgcgctccg
     2581 cgtgaagaag ggcgccggag cgttcgtctg cggtgaggaa acggccctca tggcctccgt
     2641 ggagggacgg cggggcatgc cccggccgcg gcctccattc cccgcggtct cgggcctctt
     2701 ccagaagccg accatcgtca acaacgtgga gaccatggcc aacgcctccc acatcatggg
     2761 ggaaggcgcg gagagcttcg ccgccatggg gaccgcgacc tccaagggga ccaaggtgtt
     2821 cgcgcttacc ggcaaggtgg ccaacgcggg cctggtggaa gtccccatgg gcatcacgct
     2881 tcgggagatc gtctacgaca tcggcggcgg catcccgggc ggcaaggcgt tcaaggccgt
     2941 gcagatcggc ggaccgtccg gcggctgcat tccggccgag cacctggaca tcaagatcga
     3001 ctacgagtcc ttgaagtccg tcggcgcgat catgggttcc ggcggtctgg tggtcatgga
     3061 cgaggacacc tgcatggtgg acatggccaa ctacttcctc cagttcatca cctcggagtc
     3121 ctgcgggaag tgcatcccgt gtcgggaagg cacccagcgg ctgcacgaga tcctggagtc
     3181 catcgtctcc ccgtactcca agatggcaag tgaggaggag aacctcctgc ggttcaaccg
     3241 cgtcctgaac atggagagcc tggcccaggt catcaaggac acctccctgt gcgggctggg
     3301 acagacggcg cccaaccccg ttctctccac gctgcggttc ttccgctccg agtacgaggc
     3361 gcacatctac gatcggcgct gtccggccca tgcctgcaag ggcctgctca cgtacacgat
     3421 cgacacgaac gcctgcacgg ggtgcacgtt gtgcgccaag aagtgcccca cgaacgccat
     3481 cgtgggtgag aagaagaagg cccactacat cctggaggag aagtgcatcc ggtgcggcac
     3541 ctgcaaggac gtgtgccggt tcaacgcggt gaacgtttcc tagcgcgccc gcgcgccgcc
     3601 ccgagacaag gagacatcga tggtaaccat cacgatcaac aaccgaaagc tccaggtgaa
     3661 gccgggcatc tcgatcctgg acgccgccaa ggaagccggg gagcggatcc ccaccctctg
     3721 tcacatccag gagctctttc ccagcggggc ctgccgcatg tgcgtggtgg aggtgaaggg
     3781 caagcccaac ctcaccccgt cctgcgcttt tcccgcggaa gaagggatgg agatccagac
     3841 gcgcagcccg cgc
//
LOCUS       DASRZS010000136         4723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1227018, whole genome shotgun sequence.
ACCESSION   DASRZS010000136 DASRZS010000000
VERSION     DASRZS010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227018"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFH83_03395"
     CDS             complement(<1..710)
                     /locus_tag="VFH83_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HET6485438.1"
                     /translation="MTGVLAAIAILIFPGGLFVIVGSLAYEYVDRKLIARFQNRLGPR
                     WFQPLADFVKLLVKEEIVPEGVNRGLFIWLPAVALAAALTAALYVPMAGLSPSWSFRG
                     DVVATIYLLMMMTLCIGLAGANTTDRFSVVGAVRALTQLFSYEAPFLLALLGPAVATG
                     SWRIADIDKYAQGHLWMILIQPLGFLVALVGLMGKLELPPFDAPEAETEIISGALTEY
                     SGRGLAYFRLGRDVELFIG"
     gene            complement(710..2476)
                     /locus_tag="VFH83_03400"
     CDS             complement(710..2476)
                     /locus_tag="VFH83_03400"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET6485439.1"
                     /translation="MSDPTSILKPWSLSVSARGENRLDVEIAPSSLVSAVTEIKRARW
                     GYLSAITGRDCTEADGGGSDGAVSAAAPAPLEILYHFSQGADVLTLRTKVPREGASIP
                     SVCAVLPSAILQERELAETFGIRVIDIPQDERLLLSDDWPDGLYPMRKEHTSEKLREV
                     VTGLTASASGAAAATAPSAVPLDVPRWKPGPAKPVEEKPGRFVIPIGPQHPALKEPAH
                     FELVVDGEVVTDATLRLGYVHRGIEKAAESQTWVQNLYLMERICGICSHIHATAYALG
                     VEKLAGITAPPRAQAIRLIFAEMERLHSHLLWLGVAAHQAGYDTLFMFSWRDRETIMD
                     LLDALSGNRVNYSANLVGGVKYDIEPKQADGIRRGLDFIEPRLQHYLKIVTTDESFLR
                     RTRGVGIFPKEQAEIMGVVGPTARASGVRRDLRVDAPYLGYREFPIVPVFDDACDLAA
                     RFVVRIKELFESCRIIRSALDRLPAGDLTVGVPRRIPEGEAISRVEAPRGELFYYLRS
                     TGGAKPDRVKVRTPSLANWAYVLGVAVGHRLADIPMIVVGIDPCFSCNDRMIRVRRAG
                     EERTMSWEDLRQYGIRFYGGNP"
     gene            complement(2473..3006)
                     /locus_tag="VFH83_03405"
     CDS             complement(2473..3006)
                     /locus_tag="VFH83_03405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HET6485440.1"
                     /translation="MSTTLSHPIRQALDKVALWARINSPWVVHFNSGSCNGCDIEILA
                     TLAPRWDLERFGVKLWGSPRHADILMCTGPVSRQARERLLRTYEQMPSPKFVIAVGSC
                     GITGGAFRACYNIVGDIGDVIPVDVYIPGCPPRPEAIIDGVVQLLTKLKTGAAPKAAL
                     PGTARGPDAPPASGGAT"
     gene            complement(3020..3331)
                     /locus_tag="VFH83_03410"
     CDS             complement(3020..3331)
                     /locus_tag="VFH83_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485441.1"
                     /translation="MIHPFSPPVGFAVYLVVTALLLVVAGAVSARGQASTMKTSMYGS
                     GEAAQRGAASPGYRPFFMTAFFFAILHLGVLIVGSGGFTLVTGVYLIGLILSLVALAL
                     G"
     gene            complement(3333..>4723)
                     /locus_tag="VFH83_03415"
     CDS             complement(3333..>4723)
                     /locus_tag="VFH83_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET6485442.1"
                     /translation="LAADGIPLALAWRDLAAGNAPHFTYGIIALRFDGVSLLLATVVL
                     GLGLLVSLFSGPYIGHEGEQEKYHALLNTMVGAMIGLGCAGDLFNLWVWFETAAVASY
                     MLVAYHTHQPSSVEAGVKYLVQNSVGSMLVLLGIGLVFSQAGTLDLGALRELAGKGTL
                     SRVTFLAAGALFCVGFGVKIAMVPMHTWLPDAHSQAPSGISALLSGVVIETGLVALMR
                     GLSAVAGYSLSWGPILLAFGAINMIVGNLMALRQTQVKRLLAYSSLSHVGYMLLGIGT
                     AVTFGELGGAQGGMFHLLNHALMKGLAFLAAGCLLFALRLSKGDGNPLTSDDLAGASR
                     RYPLAALGLTVGVLGLGGLPPLAGFMSKWQIFAAGFRTGNPWMIALVVFALLNSLLSL
                     GYYAPIVNIMYRHKPSAIVTEGARVPPAMAIPLVVLILAVLVLGLWPSLADPLTAPAG
                     QAVLGLFQAGGM"
BASE COUNT          856 a         1593 c         1577 g          697 t
ORIGIN      
        1 ccgatgaaga gctccacgtc tctccccagc cgaaagtagg ccagcccgcg cccgctgtac
       61 tccgtgaggg ctcccgagat gatctcggtc tccgcctccg gcgcgtcgaa gggcgggagc
      121 tcgagcttgc ccatcaggcc caccagggcc accaggaagc ccagcggctg aatcaggatc
      181 atccagaggt gaccctgcgc gtacttgtcg atgtccgcga tccgccagct tccggttgcc
      241 acggcgggtc ccaggagcgc cagcaggaag ggcgcctcgt acgagaagag ctgcgtcagg
      301 gcgcgaaccg cgccgaccac ggagaagcgg tccgtggtgt tggcgccggc caggccgatg
      361 cacagggtca tcatcatcag gaggtagatg gtggccacca cgtcgccacg gaagctccag
      421 gacgggctca ggcccgccat gggaacatac aacgccgcgg tgagcgcggc cgccaacgcc
      481 accgccggca gccagatgaa gagcccgcgg ttcacgccct ccgggacgat ctcctccttc
      541 acgaggagct tcacgaagtc cgccaggggc tggaaccagc gggggcccag ccggttctgg
      601 aacctggcaa tgagctttcg gtccacgtac tcgtaggcca ggcttcccac gatcacgaag
      661 agcccgcccg gaaagatcag gatcgcgatg gcggccaata ccccggtcat tacggattcc
      721 ctccatagaa gcggatgccg tattgcctca ggtcctccca gctcatggtg cgctcctctc
      781 ccgcgcgccg cacccggatc atccgatcgt tgcaggagaa gcagggatcg atccccacga
      841 cgatcatcgg gatatccgcc aggcggtggc ccacggcgac gcccaggacg taggcccagt
      901 tggccaggct cggcgtccgc accttcacgc ggtccggctt ggccccgccg gtgcttctca
      961 ggtagtagaa gagctctcct cggggcgcct cgacacggct tatggcctct ccttcaggga
     1021 tccggcgggg cacgccaacg gtgaggtctc ccgcggggag ccgatccagc gccgagcgga
     1081 tgatccgaca ggattcgaag agctccttga tgcggaccac gaaccgggcc gcgaggtcac
     1141 aggcatcgtc gaacaccggc acgatgggga actcccgata gccgaggtag ggcgcatcca
     1201 cgcggaggtc ccggcgcact ccagaggctc gcgccgtcgg gccaaccacg cccatgatct
     1261 ccgcctgttc cttggggaag atcccgacgc cgcgagtccg ccgcaggaag ctctcgtccg
     1321 tggtcacgat cttcaggtag tgctggagcc gaggctcgat gaagtccagg ccacggcgga
     1381 tgccgtccgc ctgcttgggc tcgatgtcgt acttcacccc tcccaccagg ttggcggagt
     1441 agttcacccg gttgccgctc aacgcgtcca ggaggtccat gatggtttcc cggtcccgcc
     1501 agctgaacat gaaaagcgtg tcataccccg cctggtgcgc ggcgacgccc agccagagca
     1561 ggtggctgtg caggcgctcc atctccgcga agatcaggcg gatggcctgc gcgcgcggcg
     1621 gcgccgtgat ccccgcaagc ttttccacgc ccagggcgta ggcggtggca tggatgtgcg
     1681 agcagatgcc gcagatgcgc tccatcaggt agagattctg cacccaggtc tggctctccg
     1741 cggccttctc gatgccgcgg tgcacgtacc ccagccgcag cgtggcatcg gtgaccactt
     1801 caccgtcgac cacgagctcg aagtgcgcgg gttccttgag cgctggatgc tgaggcccga
     1861 tggggatcac gaatcggccc ggtttctcct cgaccggctt tgccggtccg ggcttccatc
     1921 gaggcacatc caaagggacg gccgagggtg ccgtggcggc cgcggcgccc gacgcgctgg
     1981 ccgtgaggcc cgtgacgacc tctcggagct tctccgaggt gtgctccttc ctcatgggat
     2041 agagaccgtc gggccagtcg tccgagagca gcaggcgctc gtcctggggg atgtcaatca
     2101 cccggatccc gaaggtctcc gcgagctccc gctcctggag gatcgcggag ggcagcaccg
     2161 cgcagacgct ggggatggag gccccttctc ggggtacctt ggtgcgaagc gtgagcacgt
     2221 cagccccttg agagaaatgg tagaggatct ccaggggcgc gggagcggcc gcggaaacgg
     2281 caccgtccga gccaccacca tccgcctccg tgcagtccct gcccgtgatg gcggacagat
     2341 acccccaccg cgcgcgcttg atctcggtca ccgccgaaac gaggctggag ggggcgatct
     2401 ccacgtccaa ccggttctcg ccccgcgcgc tcaccgagag agaccagggt ttcaggatcg
     2461 acgtggggtc gctcatgtgg cacctccgga agcagggggg gcgtccggtc cccgcgcggt
     2521 cccgggcagc gccgccttgg gcgccgcgcc ggtcttcagc ttggtgagaa gctgcacgac
     2581 cccgtcaatg atcgcttcgg gtcgcggcgg gcagccgggg atgtagacat ccaccggtat
     2641 cacgtccccg atgtccccga cgatgttgta gcaggcgcgg aacgcgccgc cggtgatgcc
     2701 gcaggagccg acggcgatca cgaacttcgg ggagggcatc tgctcgtagg tccgcagcag
     2761 ccgctcccgg gcctggcggc tcacgggccc cgtgcacatg aggatgtcgg cgtgcctcgg
     2821 cgagccccag agcttgacgc cgaagcgctc caggtcccag cgcggcgcca gcgtggccag
     2881 gatctcgatg tcgcagccgt tgcaggaccc gctattgaag tgaacgaccc acggggagtt
     2941 gatccgcgcc cagagagcca ccttgtccag cgcctgccgg atggggtgtg acaacgtagt
     3001 gctcatgcag cctccttgct caaccaagcg cgagcgccac caggctcagg atcagcccga
     3061 tgaggtacac gcccgtgacc agggtgaaac cgccgctccc cacgatgagt actcccaggt
     3121 gcaggatcgc aaagaagaac gcggtcatga aaaacggacg atatccgggt gatgcggcgc
     3181 cgcgctgggc ggcctcgccg cttccgtaca tgctggtctt catggtgctg gcctggcctc
     3241 tcgccgagac cgcgccggca accaccagga ggagcgcggt gaccaccagg tacaccgcga
     3301 aaccgaccgg aggagagaag gggtggatca tgctacatcc ctcccgcctg gaagagaccc
     3361 aggaccgcct ggcccgccgg cgccgtgagg gggtctgcca gggacggcca cagacccagg
     3421 accagcactg ccaggatcag gaccaccagc ggtatggcca tggccggggg gaccctcgcc
     3481 ccttccgtca cgatcgccga tggcttgtgt cggtacatga tgttgacgat gggcgcgtag
     3541 tagccgaggg agagcaggct gttaaggagc gcgaagacca ccagcgcgat catccacggg
     3601 tttcccgtgc ggaaccctgc cgcgaagatc tgccacttcg acatgaagcc agcgagcggc
     3661 gggagaccgc ccaggcccag caccccgacg gtcagtccca gcgctgccaa cgggtagcgc
     3721 cgcgaagcgc ccgcaaggtc atccgaggtg agcgggttgc cgtctccctt cgagaggcgc
     3781 agcgcgaaga gcaggcagcc ggcagccagg aaggccagcc ccttcatgag cgcatggttc
     3841 aggaggtgga acatcccgcc ctgggctcct cccaactctc cgaacgtgac agccgtgccg
     3901 atgcccagga gcatgtagcc cacatggctg aggctggagt aggcgagcag gcgcttcacc
     3961 tgggtctgcc tcagggccat caggttgccc acgatcatgt tgatggcgcc gaacgccagg
     4021 aggatgggcc cccagctcag tgagtagccg gccacggcgg agaggccccg catcaaagcc
     4081 accagcccgg tctcgatcac gacgccggac agcagggcgc tgatgccgct gggagcctgg
     4141 gaatgcgcgt ccggcagcca cgtatgcatc gggaccatgg cgatcttcac gccgaacccg
     4201 acgcagaaga gcgcccccgc ggcaaggaac gtcacgcgag agagggtgcc cttgccggcg
     4261 agctcccgca gcgccccaag gtccagcgtc cctgcctggg agaagaccag cccgatgccg
     4321 agaagcacca gcatggatcc gaccgagttc tgcaccaggt acttcacgcc cgcctctacg
     4381 ctggaaggct ggtgcgtgtg gtaggcaacc agcatgtagg aggccacggc cgcggtctcg
     4441 aaccacaccc agaggttgaa gaggtcgccg gcgcagccca acccgatcat cgccccgacc
     4501 atggtgttga gcagggcgtg gtatttctcc tgctcccctt cgtggccgat gtaaggaccc
     4561 gagaagagcg ataccagcaa tcccaggccc aggacgacgg tcgccaggag gagactgacc
     4621 ccgtcgaatc gcagcgcgat gatgccgtag gtgaaatggg gcgcgttgcc ggcagccagg
     4681 tctctccagg caagggcaag ggggatgcca tccgcggcaa gga
//
LOCUS       DASRZS010000137         4549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5463803, whole genome shotgun sequence.
ACCESSION   DASRZS010000137 DASRZS010000000
VERSION     DASRZS010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5463803"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1658)
                     /locus_tag="VFH83_03420"
     CDS             complement(141..1658)
                     /locus_tag="VFH83_03420"
                     /inference="COORDINATES: protein motif:HMM:NF039926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HET6485443.1"
                     /translation="MKVRAAVLAESLASLVKARPWSPGVIQKLETLVKTADDYDLFRI
                     NPLRFAADRLIPEQEAIDVLLHGTKAGLFQMEWNLVCPTCGDMVESFRTLSRLNSHFH
                     CTVCNLNSEASLDDYIHVTFTISPEVREITFHHPESLSISDFHLRYHFNQSAMLPDGA
                     RFLDVVPSLVKGQAFLAPFARQRFEVETTGGTLTFNDLIHHESAAVLVDGHPAPARQQ
                     AEVIVEGSAISTDPVTVGKGKVVFTVRNRTSSKASMLIINFPPDFVKKRLRFEPYLSG
                     KRLLSTQTFRELFSSEVIKGTEGIGVRDITILFTDLKGSTALYDRIGDLPAFSLVHQH
                     FDRLGQAIRQNNGAFIKTIGDAVMACFLNPGDAVKAALTMIRDISRFNKSATNGQVIL
                     KVGIHKGPCIAVTLNDRLDYFGQTVNIASRVQGLAGAEEIYVTQSVFGFEGVRTLLRG
                     HKVFTGKARLKGIAEEMQVYRICPPAARPPVPAPGARPGGPPLGPGPSATRSPAR"
     gene            1795..2403
                     /locus_tag="VFH83_03425"
     CDS             1795..2403
                     /locus_tag="VFH83_03425"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET6485444.1"
                     /translation="MPFTLPDLPYPFNALEPHIDAKTMEIHHDKHHGTYVTRLNGALE
                     KAPAFQNKTIEDILRSLDSVPAEVRTAVRNHGGGHFNHTLFWKLLSPKGGGKPSGDLA
                     AALDKSFGSFDAFKEQFTTAATNLFGSGWAWLCADGSGKLSIVSTPNQDNPISQGLKP
                     LLGLDVWEHAYYLKYQNRRPEYIAAFWNVVSWSQVAANLSAT"
     gene            complement(2481..2672)
                     /gene="rpmB"
                     /locus_tag="VFH83_03430"
     CDS             complement(2481..2672)
                     /gene="rpmB"
                     /locus_tag="VFH83_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET6485445.1"
                     /translation="MARRCDICGKGTVTGNKVSHAKNRTRRVWKPNLHKIQAMVGRTK
                     TTMKICTRCLRSGKVVKAV"
     gene            complement(2899..3660)
                     /gene="cobS"
                     /locus_tag="VFH83_03435"
     CDS             complement(2899..3660)
                     /gene="cobS"
                     /locus_tag="VFH83_03435"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HET6485446.1"
                     /translation="MRRFFSALSFLTVIPVPQSLKSTRDNGMFAGYPAVGLLLGILYS
                     VLLFCARFLLSMPISTVLLLAVMLFLTGAIHLDGLADCADAFYGHRDRETTLRILKDP
                     RIGTMGGAAIGLSLLARYAALVSIPQTPVLLALPFATAFSRTSVLMAMRALPYVRSTG
                     ILNPTVSVTRPLVALACLVILAILIAFPIPTAAAALALALFWRLSWKRIGGCTGDVLG
                     ATIEIAEIVFLVAFAGAEALGFPLGGVWGLLGQRV"
     gene            complement(3705..>4549)
                     /gene="cobT"
                     /locus_tag="VFH83_03440"
     CDS             complement(3705..>4549)
                     /gene="cobT"
                     /locus_tag="VFH83_03440"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HET6485447.1"
                     /translation="EGVSAYPAEVTGMMVRGFVTGHAGINVLSRHAGIDVQVVDMGCS
                     ADFQDLVAAGSIVDAKIARGTANMRREPAMSREQALLAITRGAEIARKAALAGYRMLG
                     TGDMGIGNTTPSAAIAAVATGRPVRELTGRGTGVDDAGLARKQTVIEQSIQERRPDPR
                     DPLDILSKVGGFEIGGIAGLILGAAQSRIPVVIDGVISTAGAVIAWLLDQRVGGYMFA
                     AHRSVEPAQSAMLAMMRLEPILDLGMRLGEGTGCAVAMPIIEAGAKVISQMATFAEAG
                     VPQG"
BASE COUNT          847 a         1451 c         1476 g          775 t
ORIGIN      
        1 gcaccagcag cccgtggtga aggacggcgc cgtggtcccc gcggcggtgc tgcccctgtc
       61 cctggtgatc gaccaccgga tggtggacgg cgtctatgca tgcaagttcc tgagccggtt
      121 catggagctc ctgtcgaaac ctatcgggct ggtgatcgag tagcggaagg gccgggaccc
      181 aggggcggcc cgcccggccg cgcgcctggt gcggggaccg gcgggcgagc ggccggcggg
      241 cagatccggt agacctgcat ctcctccgca atccccttga gccgcgcctt gccggtgaag
      301 accttgtgcc cccgaagcag cgttctcact ccctcgaacc cgaacacgga ctgcgtgacg
      361 tagatctcct cggctcccgc caacccctgg accctcgagg cgatgttcac cgtctggccg
      421 aagtagtcca gccgatcgtt caaagtcacg gcgatgcacg ggcccttgtg gatgccaacc
      481 ttcaggatca cctggccgtt ggtggcgctc ttgttgaagc gcgagatgtc tcggatcatg
      541 gtcagagcgg ccttgaccgc gtccccgggg ttgagaaagc aggccatcac cgcgtccccg
      601 atcgtcttga tgaaggcgcc gttgttctgg cggatggcct ggcccagccg atcgaagtgc
      661 tggtgcacca gggagaaggc cggcagatcc ccgatccgat cgtagagcgc cgtggaaccc
      721 ttcaggtccg tgaagaggat cgtgatgtcc cggacgccga tgccttccgt ccccttgatc
      781 acctcggagc tgaagagctc gcggaaggtc tgcgtggaca ggaggcgctt gcccgacagg
      841 taaggctcga accgcaggcg ctttttgacg aagtccggcg ggaagttgat gatcagcatc
      901 gaggccttgg acgacgtgcg gtttcggacg gtgaagacga ccttgccctt gcccacggtc
      961 accgggtccg tgctgatggc cgagccctcc acgatcacct ccgcctgctg acgcgcggga
     1021 gccggatgtc cgtccacgag gaccgcggcg ctctcgtggt ggatcaggtc gttgaaggtg
     1081 agcgtgccgc ccgtggtctc cacctcgaac cgctggcggg cgaacggggc caggaacgcc
     1141 tgccccttca cgaggctggg gaccacgtcc aggaaacgcg cgccgtcggg cagcatggcg
     1201 ctctggttga agtggtagcg gaggtggaaa tcggagatgg acagcgactc gggatgatgg
     1261 aaggtgatct cgcggacctc aggggagatc gtgaacgtca cgtggatgta gtcgtccagg
     1321 ctcgcctcgg agttcaggtt gcagacggtg cagtggaaat gggagttgag gcgggagagg
     1381 gtgcggaagc tttccaccat gtccccgcag gtaggacaga cgagattcca ctccatctgg
     1441 aaaaggcccg ctttggttcc gtggagcagg acgtcgatgg cctcctgctc cgggatcagg
     1501 cggtccgcgg cgaaccgcag gggattgatc cggaagaggt catagtcgtc cgcggtcttg
     1561 accagggttt cgagcttctg gatcaccccc ggtgaccacg gccgcgcctt caccagactc
     1621 gccagactct ccgcaagaac cgctgccctg actttcacgg aacgattcct cctgcgaccg
     1681 tttttctgaa accttgcctt tgcctatacc tgactaaaat actacttttt acctgccgtt
     1741 tatgccggcc gcgcgatccg tcgcgcggtt aggcaaagtg attggaggaa taagatgcct
     1801 tttaccctac cggacctgcc ctatcctttc aacgccctcg agccgcacat cgacgccaag
     1861 accatggaga tccaccacga caaacatcat ggcacgtacg tgacccggct caacggggcc
     1921 ctggagaagg cccccgcgtt ccagaacaag accatcgagg acatcctgcg atcgctggat
     1981 tccgtgcctg cggaggtacg aacagccgtt cgcaaccatg gcggcggtca tttcaaccac
     2041 actctcttct ggaagctgct ctccccgaag gggggcggaa agccgtccgg agacctggcg
     2101 gcagcgctgg acaagtcctt cggcagcttt gatgcattca aggagcagtt cacgaccgcg
     2161 gccacgaatc tgttcggaag cggctgggcg tggctctgcg cggatggctc ggggaagcta
     2221 tcgatcgtga gcacgccaaa ccaggacaac ccgatctctc agggcctgaa gcccctcctg
     2281 ggtctggacg tgtgggagca cgcgtattat ttgaagtatc agaaccgacg gcccgagtac
     2341 atcgcggcgt tctggaatgt ggtttcctgg agtcaggtag ccgccaacct gtccgcaacg
     2401 tagttcctgc cggggccccg cggcgacgcg gggcctctat ccgccgggcc aagggaggcc
     2461 cggcccgagg tcccccgagg tcagaccgcc ttgacgacct tgccgctgcg cagacaacgg
     2521 gtacagatct tcatggtggt cttggtcctg cccaccatcg cctggatctt gtggaggttg
     2581 ggcttccaga ccctacgggt ccggttcttc gcgtggctca ccttgttccc ggtgaccgta
     2641 cctttaccgc atatatcgca cctgcgcgcc atggtttccc tcgcctttat tctcgtagtg
     2701 agaccggaac cgaaaaccta accaaaaagg cccagcgtgt aaagggggct cggccccagc
     2761 gctccctgga aatgcaccca ctcaagagat tccgggagca taggcccgac cctctcgaaa
     2821 tctacgaaat cgcaacgtac gttttcgtcg aaaccaggac ctttggaccc gtccgccgcc
     2881 gctcggccct cccccgcctc agacccgctg cccgagaagg ccccaaaccc caccgagggg
     2941 gaacccaagc gcctccgcgc cggcaaatgc caccagaaac acgatttcag cgatctcgat
     3001 ggtagctcca agtacgtccc cggtgcatcc accgatccgc ttccaactga gcctccagaa
     3061 gagcgcaagc gcgagcgccg ccgcggcagt aggtatagga aaggctatca ggatcgccag
     3121 aatcaccagg cacgcgagcg ccacgagtgg tcgggtcact gatacggtgg ggttgagaat
     3181 cccggtgctg cgtacgtagg gcagtgcccg catcgccatc aggactgagg tccgggaaaa
     3241 ggcagtggcg aacgggagcg ccagcagcac cggggtttga gggatggaaa ccagggcggc
     3301 gtaccgagcc aggagggaga gcccgattgc ggcaccgccc atggtgccaa ttctcgggtc
     3361 cttgagaatg cgcagggtcg tctcgcggtc ccgatgacca tagaaggcgt cggcgcaatc
     3421 cgcaagaccg tccaggtgga tcgcgcccgt caggaagagc atgaccgcca ggagaaggac
     3481 ggtagagatg ggcatggaaa ggaggaatcg ggcgcagaag agaagaacgg agtagaggat
     3541 accaagcagc agacccaccg ctgggtagcc ggcaaacatg ccgttgtccc gggtgctctt
     3601 cagagactgg ggaacgggga tgacggtgag gaaggagagc gccgagaaaa atcgccgcat
     3661 ggtgttcaga gatcggggcg gtccacgccg tggctcggac ggtctcagcc ctgggggacg
     3721 ccggcttcag cgaaggtggc catctggctg atgaccttgg cgccggcctc gatgattggc
     3781 attgctaccg cgcagccggt accctcgccc agccgcatgc ccaggtccag aatgggctcc
     3841 agacgcatca tggcgagcat ggcggactga gccggctcca cggagcggtg agcggcgaac
     3901 atgtagccac ccacgcgctg gtccagcagc cacgcaatga ccgcaccggc ggtggagatc
     3961 acgccgtcaa tcaccacagg gatgcggctc tgggcggcgc ccaggatgag ccccgcgatg
     4021 ccgccgatct cgaacccgcc gaccttggac aggatgtcca gcgggtcccg aggatcgggc
     4081 cgccgctcct ggatgctctg ctcgatgacc gtctgcttcc gcgcaaggcc ggcgtcatcc
     4141 acgcccgtgc cgcgccccgt gagctcgcga acggggcggc cggtcgccac ggcggcgatg
     4201 gccgctgaag gggtagtgtt gccgatgccc atgtcccccg tgcccagcat gcggtagccc
     4261 gcaagagcgg ccttgcgcgc aatctcggcg ccccgggtga tcgccagcag cgcctgctcg
     4321 cgggacatgg ccggctcgcg gcgcatgttg gcggtgccac gggctatttt cgcgtcgacg
     4381 atggatcccg cggcaaccag gtcctggaag tccgcgctgc agcccatgtc caccacctgc
     4441 acgtcaatcc ccgcgtgccg ggacaggacg ttgatgcccg cgtggccggt cacgaagcct
     4501 cggaccatca tgcccgtcac ctctgccggg taggcgctca cgccttcct
//
LOCUS       DASRZS010000138         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1228956, whole genome shotgun sequence.
ACCESSION   DASRZS010000138 DASRZS010000000
VERSION     DASRZS010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228956"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VFH83_03445"
     CDS             <1..1069
                     /locus_tag="VFH83_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET6485448.1"
                     /translation="LPLELPEVKTVKPTGTGESPLAAIPEWVNTTCPKCGGPAKRETN
                     TMPQWAGSCWYYLRYLDPGNDGKLADRSKIEYWMPIDLYVGGAEHAVLHLLYARFWHK
                     FLYDIGVVNSPEPFMRLVNQGMILGEDGQKMSKSMGNVINPDDVVKDFGADVMRLYEM
                     FMGPLEVSKPWSTRGIAGVRRFVERVWRLSQLPMTDEEPPEPLLRVLHKTIKKVSHDT
                     GRIEFNTAIAQMMIYLNEIGNLEAMPRSLWTPLVLMLSPYTPHLAEELWERMGKPPSV
                     SNQTWPTWKEELTKDDLVEVVFQINGKVRARENLPMGITDADLQKRALANDRMRELLE
                     GKEVRKVIVVPNKLVNVVAAV"
     gene            1146..>2081
                     /locus_tag="VFH83_03450"
     CDS             1146..>2081
                     /locus_tag="VFH83_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET6485449.1"
                     /translation="MVDDFESQRSNSLRWLCGQPTDALLFVLASGKTVLVPWDINLAN
                     DLGVADQIIPYTDFKRSFREAVIGVLRAAGLPQKAGGPRETDPASDQRPRVAFPARTS
                     HLRYLELSGDLPGALVEATTPGFESFITEKRNVKDPSEIAVVRRAADITNELVDLLEE
                     IVRGSGDGLRELEMAQIIEREALARGAEGLGFETLAAGPARSWAIHPFPAYSNSPFGG
                     PGFSILDFGVRVDGYTSDVTMTFIRGSLSAEQERMVSLVEQAYTAAMGALRSGVSCRE
                     PALRADEVFSAAGQKMPHSLGHGIGLDTHESPVLRT"
BASE COUNT          368 a          688 c          694 g          331 t
ORIGIN      
        1 gctgcccctg gagctgccgg aagtgaagac cgtcaagccc accggcaccg gcgagagccc
       61 cctcgcggcc atccccgagt gggtgaatac cacgtgcccg aagtgcggcg gcccggccaa
      121 gcgggagacc aacaccatgc cccagtgggc gggctcctgc tggtactacc tgcgctacct
      181 ggaccccggc aacgacggca agctggcgga ccgatccaag atcgagtact ggatgcccat
      241 cgacctgtac gtgggcggcg cagagcacgc ggtgctccac ctgctgtacg cccggttctg
      301 gcacaagttc ctttacgaca tcggggtggt gaactcgccg gagccgttca tgcgcctggt
      361 caaccagggc atgatcctgg gcgaggacgg tcagaagatg tcgaagagca tgggcaacgt
      421 catcaacccg gacgacgtgg tgaaggattt cggcgcggac gtgatgcgcc tgtacgagat
      481 gttcatgggg ccgctggaag tctccaagcc gtggtccacc cgggggatcg ccggcgtgcg
      541 gaggttcgta gagcgcgtgt ggcgcctgtc ccagctcccc atgacggatg aggagcctcc
      601 ggagccgctc ctgcgcgtcc tgcacaagac catcaagaag gtgagccacg acaccggccg
      661 catcgagttc aacacggcca tcgcgcagat gatgatctac ctgaacgaga tcgggaatct
      721 ggaggcgatg ccgcgctccc tgtggacgcc gctggtcctg atgctgtctc cgtacacgcc
      781 gcacctggcg gaggagctgt gggaacgtat ggggaagccg ccgtcggtaa gcaaccagac
      841 ctggcccacc tggaaggagg agctgactaa ggacgacctg gtggaggtgg tcttccagat
      901 caacggcaag gtccgggccc gggagaacct gcccatgggc attacggatg cggaccttca
      961 gaagcgggcg ctggccaacg accggatgcg ggaactcctg gagggcaaag aggtccgaaa
     1021 agtgattgtc gtgcccaaca agctggtgaa cgttgtcgcg gccgtctgag gagcaggtcc
     1081 gcgttttccg cggaaggcag gcggctctct ccgcctggct ctccgcgcaa ggtctctttg
     1141 cctgcgtggt agacgacttc gaaagccagc gctccaactc ccttcgctgg ctctgcggcc
     1201 agcccaccga cgcgcttctg ttcgtgctgg cctccggcaa gacggtgctg gttccctggg
     1261 acatcaacct ggccaacgat ctgggcgtag ccgaccagat cattccgtac acggacttca
     1321 agcgatcctt ccgcgaagcg gtgattggcg tgctgcgggc cgccggcctt ccccagaagg
     1381 cgggcgggcc gcgggagacc gaccccgcct cggaccagcg cccaagggtt gcattccccg
     1441 cccgcacatc ccacctgcgt tacctggagc tgtccggcga tctccccggg gccctggtgg
     1501 aagccaccac ccccggcttc gagtcgttca tcaccgagaa gcgcaatgtc aaggacccgt
     1561 cggagatcgc ggtcgtccgc cgggcagccg acatcaccaa cgagctcgtg gacctgctgg
     1621 aggagatcgt ccgaggaagc ggcgacgggc ttcgcgagct cgagatggcc cagatcatcg
     1681 agcgggaggc cctggctcgc ggcgccgaag ggctgggctt cgagaccctg gcagccggac
     1741 ccgcccggag ctgggccatc cacccgtttc ccgcctactc caacagcccg ttcggcgggc
     1801 cggggttctc catcctggac ttcggcgtgc gggtggacgg atacacgagc gacgtgacca
     1861 tgacctttat ccgtggcagc ctctccgccg agcaggagcg catggtctcc ctggtagagc
     1921 aggcctacac cgcggccatg ggagccctgc ggagcggcgt ctcctgtcgc gaaccggcgc
     1981 tccgggcgga tgaggtcttc tccgccgccg gccagaagat gccgcacagc ctgggccacg
     2041 ggatcggcct ggacactcac gagtcaccgg tgctccgcac t
//
LOCUS       DASRZS010000139         4349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3157399, whole genome shotgun sequence.
ACCESSION   DASRZS010000139 DASRZS010000000
VERSION     DASRZS010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3157399"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFH83_03455"
     CDS             <1..200
                     /locus_tag="VFH83_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485450.1"
                     /translation="VIAGGIDVSQLLTLGTPDQVRAECRRAIDATGGRGFLLGSTTEL
                     LPMVKVENVLAMLETARTYLP"
     gene            complement(197..1153)
                     /locus_tag="VFH83_03460"
     CDS             complement(197..1153)
                     /locus_tag="VFH83_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6485451.1"
                     /translation="MDQSPVNVILGSGGAIGTPLALQLLAEREPVVAVSRTGKAPVGA
                     RSVRADLTDAGQTEAAIPQGATVFLLVGLPYDARVWKTQWPRIIRNAATACRSKGARL
                     LFLDNVYLYGAVAGPMTEETVTRPSSEKGRIRAEVAEYLLGEMSSGRVTASIARAADF
                     YGPYATASSVPYVLALEPLLKGRRPRWLIDARKLHSYSYTLDCAKALCLLARAPDSFG
                     QVWHLPTMHPPITGEEFIRIAAKALGRPAAYSILSKPTLALAGLFNRQILELSRMTYQ
                     NDRDYLFDSTKFERRFGFQPTPYPIGIEETLRLAAPSAGQSA"
     gene            complement(1154..1999)
                     /gene="ygiD"
                     /locus_tag="VFH83_03465"
     CDS             complement(1154..1999)
                     /gene="ygiD"
                     /locus_tag="VFH83_03465"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HET6485452.1"
                     /translation="MTPKDLKRESEHLERTPRMPVLFVGHGSPMNAIEENAFSRGWRE
                     VAGTLPRPRAILCVSAHWETDGTLVTAMERPRTIHDFGGFPEELYAARYPAPGSPSLA
                     AETRTAVRSVPVGVDERWGLDHGCWSVLMQMYPEADVPVVQLSLDTGKPGSYHFELAR
                     ELAPLRDRGILIIGSGNLVHNLRRMEIRGGSGDDFNTPYGFDWALDANALFKKLIDEE
                     RYGELGNYRGLGSAVQLAVPTPDHYYPMLYAVGLKQKGETIRYFNDEPVAGSLTMTSM
                     VIGEE"
     gene            2105..3115
                     /locus_tag="VFH83_03470"
     CDS             2105..3115
                     /locus_tag="VFH83_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6485453.1"
                     /translation="MEKRRCGSSGLELPVVGVGVWAFGGGSYWGEQDQGEVDRIVARA
                     LDLGCGFFDTAEMYNEGRSEESLGKALAGRRSQALICSKIPPDHCQPEAVIQHCDASL
                     RRLGTDHVDLYLIHWPLTTVKNPPRLDQTIDAMRSLQAAGKVRFIGVSNYGPRQLEEL
                     RSAGAHPACNELPYSLLARAIEHALMPVCERTGIGIIAYMPLMQGLLTGRYASADDLP
                     ANRARTRQFRGDRPGSRHGEPGFEELTFRAVRAIRGLCDQAGMPMERAALAWCAARFG
                     VTCTIAGARTVAQLEENAKAGETRLSDDLLSALDAATDELKQAMGPSVDLFESAANTR
                     TW"
     gene            complement(3096..3926)
                     /locus_tag="VFH83_03475"
     CDS             complement(3096..3926)
                     /locus_tag="VFH83_03475"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6485454.1"
                     /translation="MIDDPTGGARIEPAIRGGAADDPMARAKIDCSVGFGRWPFARFS
                     EDTPARLDRLLAGNGIQAALVSSIEAILYEEPEEWNRDLARRLRGYPRLLPIPVANPR
                     VRSVASIVAEPGLKAVKLIPNYHGYSLGDDRTVELCEALADRGVPVLVQMRVEDERSH
                     YELLKVPGVPVPDIVTLTRALPNLAVVALGPYFAEAIPLAQAGVYVDISFAETLDTLG
                     QLCKKAPVERILFGSHAPWMYPRAASAKLARSTITEEQREAIGWGTASRLFGITTSGC
                     "
     gene            complement(3923..>4349)
                     /locus_tag="VFH83_03480"
     CDS             complement(3923..>4349)
                     /locus_tag="VFH83_03480"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6485455.1"
                     /translation="REVRLPLLHHSWNTRAMGRISSPGCYQSDSEDIATLAARFPTVT
                     IVAAHLRPNGLRGIWQVRDHRNVLFDTSGGQPITGVIEAAVRLLGSHRVVYGSDAYFP
                     EGRDYASQMACIEEARISESARERILGLNALRVLAEAAA"
BASE COUNT          696 a         1469 c         1482 g          702 t
ORIGIN      
        1 tggtcatcgc cggcgggatc gacgtgagcc agcttctgac cctcggcacg cccgatcagg
       61 tccgggcgga gtgccgtcgg gccatcgatg ccaccggcgg acgcgggttc ctcctgggct
      121 cgaccacgga gctcctgccg atggtgaagg tcgagaacgt cctggcaatg ctggagaccg
      181 ccaggacgta cctgccttaa gccgactgcc ccgcgcttgg cgcggcgagc cgcagcgtct
      241 cctcgatccc tatcgggtac ggtgtgggtt ggaagccgaa ccggcgctcg aacttcgtgg
      301 aatcgaaaag gtagtcccgg tcgttctggt aggtcatccg ggagagctcc aggatctggc
      361 gattgaagag ccccgccagc gccagcgtcg gtttggacag gatggaatag gcggctggcc
      421 tacccagagc cttcgcggcg atgcggatga actcctctcc ggtaatgggc ggatgcatcg
      481 tcggaaggtg ccacacctga ccgaaggaat ccggtgcccg cgccaggaga catagtgcct
      541 tggcgcaatc cagggtgtac gagtaggaat gcagcttcct ggcgtcgatg agccaccgcg
      601 gccggcgccc cttcaggaga ggctccaggg ccaggacata cgggacgctg ctcgccgtgg
      661 cgtagggtcc gtagaagtcc gcggcccgcg cgatggatgc cgtcaccctg ccggagctca
      721 tctctcccag caggtactcg gccacctcgg cgcggatcct tcctttctcg ctggagggcc
      781 gtgtaacagt ctcctccgtc atggggccag ccacggcgcc gtagagatac acgttgtcca
      841 ggaagaggag gcgcgcgccc ttggagcggc atgccgtggc ggcgttgcgg atgatccgag
      901 gccactgggt cttccagacg cgcgcgtcgt agggaagccc caccagcagg aataccgtgg
      961 ccccttgcgg aatggcggcc tcggtctggc cggcgtcggt gagatcggcc cggacggacc
     1021 gcgcccctac cggcgccttc ccggtccggg acaccgctac caccggctct cgctcggcga
     1081 ggagttggag cgccagaggg gtgccgatgg ccccgccgga gccgaggatc acgttcaccg
     1141 ggctctggtc catttactcc tccccgatca ccatcgatgt catggtgagc gacccggcca
     1201 cgggctcatc gttgaagtag cggatcgtct cccccttctg tttcaggccg acggcgtaga
     1261 gcatggggta gtagtggtcc ggcgtgggaa cggcgagctg caccgcggat ccgagccctc
     1321 ggtagttccc cagctcgccg taccgctctt catcgatcag cttcttgaaa agcgcgttgg
     1381 cgtccagggc ccagtcgaac ccgtacgggg tgttgaagtc atcgccgctg ccgccccgga
     1441 tctccatcct gcgaaggttg tgcacgaggt tcccgcttcc gatgatgagg atccctcggt
     1501 cccgcaaggg cgccagctct cgagccagct cgaagtgata gctgccgggc ttaccggtat
     1561 ccaggctgag ctggaccacg ggcacgtccg cctccggata catctgcatc agcacgctcc
     1621 agcatccgtg atccaggccc caacgctcgt cgacccctac gggcacgctt ctgacggcgg
     1681 tccgcgtctc agcagccagg ctcgggctgc ccggagccgg gtaccgggcg gcgtacaatt
     1741 cctcgggaaa ccctccgaag tcgtggatgg tcctgggcct ctccatcgcg gtcaccaggg
     1801 taccgtcggt ctcccagtgg gcggacacgc agaggatggc ccggggtcgg ggaagcgtcc
     1861 ccgccacctc acgccagccg cgcgagaacg cgttctcctc gatcgcgttc atcgggcttc
     1921 cgtgccccac gaagagcacc ggcatccgcg gcgtgcgttc caggtgctcc gactcccgtt
     1981 tcaggtcctt tggcgtcatg gctcctccgt ggctactact cctgcccttg aatatagtag
     2041 atagtgactg cgtcaggaac gcaggggctg cgtcggatac cgagcccggg ggagagaaac
     2101 gcggatggag aagcgacggt gcggatcgtc agggctcgaa ctgccggtgg tcggcgtggg
     2161 cgtctgggcg ttcggcggcg gttcctactg gggagagcag gatcagggcg aggtcgaccg
     2221 gatcgtggcc cgggccctgg acctggggtg cgggttcttc gacaccgcgg agatgtacaa
     2281 cgaggggcga agcgaggagt ccctgggcaa ggccctggcc ggacgacgct cccaggcgct
     2341 gatctgctcc aagatcccgc ccgatcactg ccagccggag gcggtcatcc agcactgcga
     2401 cgccagtctg aggcgcctgg gcaccgacca cgtcgacctg tacctcatcc actggccgct
     2461 caccaccgtg aagaatccgc ctcgcctgga ccagaccatc gatgcgatgc gcagcctgca
     2521 ggccgcgggc aaggtgcggt tcatcggcgt cagcaactac gggccgcgtc agctggagga
     2581 gcttcggagc gccggagccc accccgcgtg caacgagctg ccctacagcc tgctcgcgcg
     2641 cgccatcgag cacgccctga tgcccgtctg cgagcgcacg ggcatcggga tcatcgccta
     2701 catgcccctg atgcagggcc ttctcacggg ccgctatgct tcggcggacg atctgcccgc
     2761 caaccgggcg cgcacccggc agtttcgggg agaccgtccg ggctctcgac acggggaacc
     2821 gggattcgag gagctgacgt tccgagcggt ccgagcgatc cgcgggctct gcgaccaggc
     2881 ggggatgccc atggagcgcg cggctctggc ctggtgcgcg gcgcgcttcg gcgtgacctg
     2941 caccatcgcc ggcgcgcgga ccgtcgcgca gctcgaggag aatgccaagg cgggagagac
     3001 ccggctctcg gacgatctcc tgtcggccct ggacgcggcc accgacgagc tcaagcaagc
     3061 gatgggaccg tccgtggacc tcttcgagag cgccgctaac acccggacgt ggtgattccg
     3121 aagagccggg acgcggtccc ccatccgatc gcctcccgct gctcttcggt gatcgtggat
     3181 cgcgccagct tggcggatgc ggcccggggg tacatccacg gcgcatgaga gccgaagagg
     3241 atccgctcga cgggagcctt tttgcagagc tgtcccaacg tgtccagggt ttccgcaaag
     3301 ctgatgtcca cgtagacgcc cgcctgcgcc agcgggattg cctccgcgaa gtaggggcca
     3361 agcgccacga ccgccaggtt cggaagagcg cgggtcagag tgacgatgtc cggcacgggg
     3421 acgcccggca ccttcaggag ctcgtagtgg ctgcgctcgt cctcaacccg catctggacc
     3481 agcaccggca cgccccggtc tgccagcgct tcgcagagct cgaccgtccg gtcatcgccc
     3541 agagaatacc cgtggtagtt ggggatgagc ttgaccgcct tcaggccggg ctccgccacg
     3601 atggaggcga cgctgcgcac ccgggggttc gccacgggta tcgggagcag ccgcggatag
     3661 ccgcgcagcc ggcgcgccag gtctcgattc cattcctcgg gctcctcgta gaggatggcc
     3721 tcgatgctgg agaccagcgc cgcctggatg ccgttaccgg ccaggagccg gtccagccgg
     3781 gccggagtgt cttcggagaa ccgggcaaac ggccagcggc cgaaccccac gctgcagtcg
     3841 atcttggcgc gggccatcgg gtcgtcagcc gcgccgccgc gaatcgccgg ctcgatcctg
     3901 gcgcctcccg tcgggtcgtc gatcatgccg cggcctccgc caggacccga agcgcattga
     3961 gccccaggat gcgctcccgt gcgctctccg agatccgggc ctcctcgatg cacgccatct
     4021 gcgaggcata gtcccgaccc tccggaaagt aggcatcgga accgtacacc acccggtggg
     4081 agcccaggag tcggaccgcg gcctcgatca ccccggtgat gggctgaccg cccgaggtgt
     4141 cgaagagcac gttgcgatgg tcccgcacct gccagatccc gcgaagtccg ttgggacgca
     4201 ggtgcgcggc cacgatggtc accgtgggga accgggccgc gagggtcgcg atgtcctccg
     4261 agtcgctctg gtagcaaccc ggtgagctga tccttcccat ggcgcgcgta ttccaggagt
     4321 ggtgcaggag cggcagccgg acttcgcgg
//
LOCUS       DASRZS010000140        10030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3926228, whole genome shotgun sequence.
ACCESSION   DASRZS010000140 DASRZS010000000
VERSION     DASRZS010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10030
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3926228"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VFH83_03485"
     CDS             <1..1225
                     /locus_tag="VFH83_03485"
                     /inference="COORDINATES: protein motif:HMM:NF023435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6485456.1"
                     /translation="VIASGENCDILFSAAWSDFGPQATKGAFVELTDLLNKYGQDILK
                     QPYAWILDAGKIGGKLYGLATYEEIAQQRGFFLRKDLVDKYHFTVKPINDPADLEPML
                     KTIQQNEPSMQPLYMQRGHLLSEMGPWDRQSDNEVTAMDVTNPKAQYVNFFDNDYYRN
                     SMNLARRWFVNGYTNKDVATSQANEYDVMRGGNTFAVGGITNAGLQDARFSAMWGVPV
                     VSFSTGKSIISTSLIQGALFVIPIQSKNRERAMMFLDRMHTDPVLVNLLVYGQEGVDY
                     VKKGPTQIDLPPGVKAGTTFSGVYWMMGNESLEYTYGNQDPNFPKLLNDWIQSAVHTP
                     ALGFTYNPEKMKNQMAALSNIGLQYDPELSSGSVDPNGGLYQELLGKLKDAGINDLLA
                     DQNQQLQAFLSASKK"
     gene            1386..3908
                     /locus_tag="VFH83_03490"
     CDS             1386..3908
                     /locus_tag="VFH83_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6485457.1"
                     /translation="MKPGGGSGARGADPAAAGAGTLKKQLVRYLFGVCAVGVAFGVRE
                     ALSLAVGPDFPEYLVFYPTVMIVALVTGLWPALFAIATTAGMIVAFWAIPLRGRLLPL
                     TTSNFVSLALFVGVCAFLSLVAELYRRNREKAAAYDKEEALRESHAVLRQQADLLKLS
                     FDAIIVWRTRGGIESWNRGAEELYGFSEQEALGRDIRDLLRTRYPTAWTEFEGVLRGR
                     RYWEGELNHVTKGGDEVVVFSRLHIGSGPDEGERVLEIDRDITEQKRVQQELQRAHDE
                     LEDKVQRRTSELRKTNRMLRMVSRCDEALVQATDEGDLTAVISQIIHDEGGYPLAWVG
                     ISETRDESRLRCAASAGDHDLGGFDPPAVEGPALEAVRSGAAVISAGEVAREGSVSPR
                     RPMAVAAFPLLDLGGAAFGALVICSDRPAEFDSTQVSLLKELADDLAFGLTSLDARKE
                     RDEAERALELKAAQLRALAGEIVRTEQRERQRIAQLIHDNLQQLLAATLYGLASLSTR
                     SRVRREEELKALREQLRDCITISRSLSSEISHPAFSEPDLVAALEWLVTWTRDKHGLE
                     VHLEPGRAVGAVPGETRIMLLQAARELLFNVVKHANVKSAWVRLDQDPQGLVKITVRD
                     EGVGFDTASLVGVGRGPGGIGLFSVRERLALAGGGLEVTSSPGNGTRVTIWAPAAQPD
                     GGRAAAMDAPPSSGIAPRSAPPRAPLVRPPGEGAAIRILLVDDHVVVRKGIGLQLAQQ
                     QDMQVVGEASDGETGIQLARELKPDIVTMDVNMPGMGGIRAVGVLHAELPELPVIGLS
                     MIDEPTQTKAILDSGAVGYVCKSAGAETLIAAIRAHARRPGA"
     gene            complement(3905..4291)
                     /locus_tag="VFH83_03495"
     CDS             complement(3905..4291)
                     /locus_tag="VFH83_03495"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HET6485458.1"
                     /translation="MRYRHKVTDRQVEWFIGGLLRAGVIAAATIAVAGGIGYLVRHAE
                     DSPQYAIFRAEPDYLRKVWAVLSGAFALRTDALIQAGLLALIAVPILRVAVSMVAFAL
                     ERDLLYSLVTALVLGILLFSLLGGRI"
     gene            complement(4293..5129)
                     /locus_tag="VFH83_03500"
                     /pseudo
     CDS             complement(4293..5129)
                     /locus_tag="VFH83_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502259.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            5330..6250
                     /locus_tag="VFH83_03505"
     CDS             5330..6250
                     /locus_tag="VFH83_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485459.1"
                     /translation="MSRFVESPAAEAVHHVRDAVSLQLLGMSRLAVDPSVRRSLHSVA
                     VRARRQLRAIERLAEADLLHPFCRIALAQEQPAPVPEGRRPRVGVYPIAADPMHWGHI
                     LVGLTAMASLRLDKVVYVIAGMDPRKPAMVAAATRHRLGRSVIDLFEPFLAYSPLALG
                     TDLDGETNFGRLLTLNERQRMDAFYIAGADHYRRTNDRGGPDTIAKLERVVEEQDCRC
                     GDHHRISVVFVRRAGVPECPSPIATFLTVHVLPALDLHFSSTAIRRALCDPTASAALA
                     SLPYACLSDIRGHGLYEHGPECVEHHAAPA"
     gene            6481..6936
                     /locus_tag="VFH83_03510"
     CDS             6481..6936
                     /locus_tag="VFH83_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6485460.1"
                     /translation="MVSLPLPPLRDRMEDIPLLVGHFISRFNRTQGRDITGMTDEALA
                     CLMSHPFPGNVRELENIIERPFILCRAGEIDRGHLPDGLCPTTASAGTSRLATHRSFK
                     QLEAAFVSSALERNGWNKLKTARELGIHKTTLFRKMRALGISGAPDPGR"
     gene            7097..7978
                     /locus_tag="VFH83_03515"
     CDS             7097..7978
                     /locus_tag="VFH83_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HET6485461.1"
                     /translation="MSRIAVAIWQGHVATTLDFAGTLLLVDVQDGTITAQSEVGLEES
                     AVRHLAGLLGGRGIQTVICGAISRPLLRSLQAGGIRVVPFVRGEAQEVLAAFLDGTLE
                     DSRFLMAGCAPGARRTVPEPKAGVVGGAIPAPERIWLKRRSPMPGRDGTGGEQRSSGL
                     RVAIPLDENRLSAHFGHGKRFALMDVDTEAKLVLSRQDFDAPEHVPGAFPRWLAANGA
                     QLVIAGTMGANAQRLLAQAGIQVLMGAPVETPEELVGAYLEGKLRLEPAPCDHGGGHH
                     DADGSTGSHAHGDDPRR"
     gene            7923..8324
                     /locus_tag="VFH83_03520"
     CDS             7923..8324
                     /locus_tag="VFH83_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6485462.1"
                     /translation="MMRTVAPVHTLTVTILVDDDGESSRTEHGLSLWVDADRFRILFD
                     SGSGGKLEENARHLGVDLAEADAFAVSHGHHDHTGGFATVLSKCGKARIYLHPGALGE
                     RYARSRDGSIRAVGCPLTPGEDLARRDPFLH"
     gene            8333..8779
                     /locus_tag="VFH83_03525"
     CDS             8333..8779
                     /locus_tag="VFH83_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6485463.1"
                     /translation="MTRLHPAVFVTGEIQRLAPTEALGDPFYLDSRCSVADPLSDDQA
                     LVVDTQDGAVVLLGCSHSGVGNTLASAMSHLSTRKLRAVVGGMHLEDASLDAMTRVGN
                     ALEELGALTICPCHCTGDKAKEYLRRRFPKAYVHGSVGTVLKLSGA"
     gene            8800..9213
                     /locus_tag="VFH83_03530"
     CDS             8800..9213
                     /locus_tag="VFH83_03530"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET6485464.1"
                     /translation="MKSTSKHVSHCYQSVVDNDRHHGLVLDLPGAKGGDDLGPTALEL
                     AVMALSGCISTIWAIVATNSKVSYDKLIVVVDADKPDDQPTTTAARALVTVSSREPLE
                     KLQRTLDRTMATCPVGKLYEKAGIAIETKLVLNGD"
     gene            9685..9999
                     /locus_tag="VFH83_03535"
     CDS             9685..9999
                     /locus_tag="VFH83_03535"
                     /inference="COORDINATES: protein motif:HMM:NF022179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HET6485465.1"
                     /translation="MKPLTERYDVSTVHNQSEERVYIRVEKALEEHPEVCRCPTCVLD
                     LVAFMLNRVTPRYSTGLLGELHPRKLLERKMGLEIELSLKAGMERLRRHPHHESGSTA
                     GA"
BASE COUNT         1775 a         3309 c         3334 g         1612 t
ORIGIN      
        1 ggtcatagcc tccggagaga actgcgacat cctgttctcc gccgcgtggt cggacttcgg
       61 cccccaggcc accaagggcg ccttcgtgga gctgaccgac cttctgaaca agtacggcca
      121 ggacatcctg aagcagccct acgcgtggat cctggatgcc ggcaagatcg gcggcaagct
      181 ctatggcctg gccacctatg aggagatcgc ccagcagcgg ggattcttcc ttcgcaagga
      241 cctggtcgac aagtaccatt tcacggtcaa acccatcaat gaccccgccg acctggagcc
      301 gatgctcaag accattcagc agaacgagcc gtccatgcag cccctctaca tgcagcgcgg
      361 gcacctgctg tccgagatgg gtccctggga ccgccagtcg gacaacgagg tcacggcaat
      421 ggacgtcacc aaccccaagg cccagtacgt gaacttcttc gacaacgact actaccgcaa
      481 ctccatgaac ctggcgcggc ggtggttcgt gaacggctac accaacaagg acgtcgccac
      541 gtcccaggcg aacgagtacg acgtcatgcg gggcggcaac accttcgcgg tgggcggcat
      601 caccaacgcc gggctccagg acgcgcgctt ctcggccatg tggggagtgc cggtcgtcag
      661 cttcagcacc gggaagtcca tcatctccac cagcctcatc caaggcgcgc tcttcgtgat
      721 cccgatccag tccaagaatc gggaacgcgc catgatgttc ctggaccgca tgcacacgga
      781 cccggtgctg gtcaacctgc tggtctacgg ccaggaaggc gtcgactacg tgaagaaggg
      841 gcccactcag atcgacctgc cgcccggagt caaggccggc accacgttca gcggcgtcta
      901 ctggatgatg ggcaacgagt cgctggagta cacctacggg aaccaggatc cgaacttccc
      961 caagctcctg aatgactgga tccagagcgc ggtccacact ccggcgctgg gattcaccta
     1021 caatcccgag aagatgaaga accagatggc ggccctgtcc aacatcggcc tccagtacga
     1081 tcccgagctc tcctcgggct cggtggatcc gaatggcggg ctgtaccagg agctgctcgg
     1141 caagctcaag gatgccggga tcaacgacct cctggccgat cagaaccagc agcttcaggc
     1201 cttcctgagc gctagcaaga agtagcgcat acgcgggtgg ggcgcagcgc cccacccggc
     1261 ctgtggcggc cgtcaccagc gtggcggccg tcaccgtttc gagaagcacc acccgcacat
     1321 ctcaggaacc tgcgcgttct ctccgctgga ttatgccgcg gccgtctcgg tatattcgag
     1381 tgagcatgaa gcccggggga gggagcggag cccgcggagc agacccggcc gccgccgggg
     1441 ccgggaccct gaagaagcaa ctcgtccggt acctctttgg cgtgtgcgcg gtcggcgtgg
     1501 ccttcggggt gcgcgaagct ctctcgctcg ccgtaggtcc cgatttcccc gaatacctcg
     1561 tattctatcc caccgtgatg attgtcgcgc tggtgaccgg tctgtggccg gcgcttttcg
     1621 ccatcgcgac aaccgccggc atgatcgtcg cgttctgggc gatccccctg aggggccggc
     1681 ttctcccact caccacgtcg aacttcgtga gtctggcgct cttcgtcggg gtgtgcgcgt
     1741 tcctgagcct ggtcgccgag ctctaccgcc ggaatcggga gaaagcggcg gcatacgaca
     1801 aggaagaagc cctgcgggaa agccacgccg tcctgcggca gcaggcggat ctcctgaagc
     1861 tttccttcga tgccatcatc gtctggcgga cccggggcgg gatcgagagc tggaaccggg
     1921 gcgcggagga gctctacgga ttctccgagc aggaggcgct ggggcgcgat atccgcgacc
     1981 tcctgcgaac gaggtatccc accgcctgga ccgagttcga gggggtgctg cgagggcggc
     2041 gctactggga aggggagctc aaccacgtca ccaaaggcgg ggacgaggtc gtcgtcttct
     2101 cccgccttca catcggctcc gggccggatg agggagagcg ggtcctggag atcgaccggg
     2161 acatcaccga acagaaacgc gtgcagcagg agctccagcg ggctcacgac gagttggagg
     2221 acaaggtgca gcgccggact tccgagctgc ggaagaccaa ccggatgctc cggatggtga
     2281 gccgctgtga cgaggcactg gtgcaggcaa cggatgaggg ggatctgacg gccgtcatct
     2341 cgcagatcat tcacgacgag ggcggttacc cgctggcctg ggtggggatc tcggagacca
     2401 gggatgagag ccgccttcgg tgcgccgctt cggcgggcga ccacgacctt ggcgggttcg
     2461 atccgcctgc cgtcgaaggt ccggccttgg aggcggtccg gtcgggagcg gccgtgatct
     2521 ccgccggcga ggtggcgcgg gagggaagcg tctccccgcg gagacccatg gcggttgccg
     2581 cctttcccct gctggacctt ggcggggccg ccttcggggc cctggtcatc tgctcggacc
     2641 gtcccgccga gttcgactcg acgcaggtca gcctcctcaa ggagctggcc gacgacctgg
     2701 cgttcggcct gacctcgctc gatgcgcgca aggagcgcga cgaggcggag cgcgccctgg
     2761 agctgaaggc ggcgcagctg cgggctctgg cgggggagat cgtgcggacg gaacaacggg
     2821 agcgccagag aatcgctcag ctcatccacg acaacctaca gcagcttctg gccgcgacgt
     2881 tgtatggact ggcgagcctc agcacgcgct cccgagtcag acgggaggag gagctgaaag
     2941 ccctccggga gcagcttcga gactgcatca ccatctcacg ctcgttgagc tcggagatca
     3001 gtcatcccgc cttctcagag ccggacctgg tcgccgccct ggaatggctg gtcacctgga
     3061 ccagggataa gcacgggctg gaggtgcacc tggagccagg gagggccgtg ggtgccgtgc
     3121 ccggtgagac gcggatcatg ctgctccagg ctgctcggga gctcctcttc aacgtggtca
     3181 agcacgccaa cgtgaaaagc gcgtgggttc ggctcgacca ggatccccag gggctcgtga
     3241 agataaccgt ccgtgacgag ggggtgggct tcgacactgc ctcgctcgtc ggggtcggga
     3301 gaggtccagg cggcatcggc ctgttcagcg tccgggagag gctggcgttg gccggcggcg
     3361 gcctcgaggt gacgagctct cccggcaacg gaacccgcgt cacgatctgg gcccccgccg
     3421 cgcagccgga tggcgggaga gcggcggcga tggacgcccc gccctccagc ggaatcgcgc
     3481 cgcgcagcgc gccgccacga gcgcctctcg tcaggccccc gggggaaggg gccgccattc
     3541 ggatcctgct cgtggacgac catgtcgtgg tccgcaaagg aatcggcctg cagctcgccc
     3601 agcagcagga catgcaggtc gtgggggagg cctcggatgg cgagaccggc atccagctgg
     3661 cgcgcgagct gaagccggac atcgttacca tggacgtcaa catgccgggg atgggtggaa
     3721 tccgggcggt tggcgtcctg cacgccgagc tcccggagct gccggtgatc ggcctttcca
     3781 tgatcgacga gcccacgcag accaaggcca tcctggattc cggcgcggtc gggtacgtgt
     3841 gcaagagtgc gggcgccgag accctgatcg cggcgatccg ggctcacgca aggcgacccg
     3901 gcgcctagat cctccctccc aggagagaga atagcaggat gcccagcaca agagcggtga
     3961 ccagggagta cagcaggtct cgctccaggg cgaaggccac catggaaact gcaacgcgga
     4021 ggatcgggac cgcgatcagc gcgaggagcc ccgcctggat cagggcgtcc gtccgcagag
     4081 caaaggcccc cgacagcacg gcccagacct tccgcagata gtcgggctcg gcgcggaaga
     4141 tggcgtactg cgggctgtct tcggcgtggc ggacgaggta gccgatgccg ccggctaccg
     4201 cgatggtggc ggccgcgatc acgccggcgc gcagcagccc gccgatgaac cattcgacct
     4261 gtcggtccgt gaccttgtgt cggtacctca tctcacaacc ttccggagaa gcccttgtag
     4321 atcatctcga tgccgaggag cacgatggtg acggcaaacg cgatccgcag agcgcgggtc
     4381 ggagcgccgg tgagcacgcg cgatcctgcc agggatccgg ccagcacgcc gaccatgatg
     4441 ggcaaggaca acactggatc gatatagccg cggctcaact acacccccgc gctcgccgcg
     4501 gctgtcaccc cgatcatgaa gttgctcgtc gtggtggaga ccttgaacgg cagcttcatc
     4561 acgcgatcca tggcgatcac cttgagggcg ccggatccaa tgcccagcag tcccgagagt
     4621 actcccgcca gcgccatcag accgaaaccc gccggaaccg agcgcacatg gtagtgccgc
     4681 ggaccttccg gggtggggta gctcccgtcc atgcgaagca acactcccag cttgctggcc
     4741 tctgccgccc cgccgtcacc gggcggcgcg cgaaagctga ggtaagcgga gtacagcagt
     4801 acgcccccga aaatgacggc gataaccccc ggcgccacgt ggagcgccac gatcgcgccc
     4861 cccagcgctc ccagcgtggt ggcaacctcc aggaacatcc cgacgcgtat gttgctgaag
     4921 ccttcgcgga cgtaggcggc ggccgccccg gacgaggtgg cgatcacgga gaccagcgag
     4981 gcgccgatgg cgtaccggat atccacgccc aggaggagcg acagaagggg tatgatgatg
     5041 accccacccc cgagtccggt caacgacccg atgaacccgg cgaccgtgga gccgcacgcg
     5101 agcaaggccg tcaactgcca ggtattcagg ctacgctccg gcctggccgg ccgctgctac
     5161 ggtaaccacg gcggcgatct ctgggtacgc gccagggcgt gaggttcatg agctgcctag
     5221 aagatacgtg caccgccggg caagtcaaca tctcgaagga ccgcggccgg ggtgcgtaac
     5281 ttttcggata ccggcggtgt tgctctgtgt ggtacgaacg aggcatcgct tgagccgttt
     5341 cgtcgaaagc ccggccgcgg aggcggtcca tcacgtccgc gacgcggtga gtctgcagct
     5401 tcttggaatg tcccgattgg cggtcgatcc gtccgtcaga cggagccttc actccgtggc
     5461 cgtgcgcgcg cgcaggcagc ttcgagcgat cgagcgtctc gccgaggcgg acctcctcca
     5521 cccgttctgt cgcatcgcgc tggcgcagga acagcccgcg cccgtcccgg aagggcggcg
     5581 gccccgcgtt ggggtgtacc cgatcgcggc ggatcctatg cactgggggc acatcctggt
     5641 cgggctcacg gcaatggcct ccctgaggct ggacaaggtg gtctacgtca ttgccggtat
     5701 ggacccgcgc aagcccgcga tggtggcggc cgcaacccgc caccgcctgg gccggtccgt
     5761 gatcgacctg ttcgagccgt tcctcgccta ctctccgctg gccctcggta ccgacctgga
     5821 cggggagacg aacttcggca ggctgctgac gctgaacgag cggcagcgga tggacgcgtt
     5881 ctacatcgcc ggcgcggacc actaccgcag gacgaacgat cgcggcgggc cggatacgat
     5941 cgccaagctg gagcgcgtcg tcgaagagca ggactgccgg tgcggtgacc atcaccggat
     6001 ctccgtggtt ttcgtccgtc gcgcgggcgt gccggagtgc cccagcccca tcgccacgtt
     6061 cctcacggtg cacgtcctcc cggccctgga cctgcacttc tcctcgacgg cgatccgccg
     6121 ggccctctgc gatccgaccg cttcggcggc cctggcttcg ctgccgtacg cgtgcctcag
     6181 cgatatccga gggcacgggc tgtatgagca cgggccggag tgtgtggaac accacgcagc
     6241 gccggcttga ccagacgtcg agccttgccg ctgcgccgtg tccccttgcg ccttcgtgcg
     6301 gccggggtgt acgctgaatg cgagatgctt cgcctgctcg aatggccccg gagatgatgg
     6361 aaggcatcca agccatcatc ctggactcca tcacggaagg ggtcttcacc atcgatggtt
     6421 ccttccggat cacctccttc aacagcgccg ccgagaagat cacggttcta ccggatcaac
     6481 gtggtctcac tccctttgcc tccgctgcgg gaccgcatgg aggacatccc gctcctcgtc
     6541 gggcacttca tctcacggtt caaccggacc cagggccgcg acatcaccgg catgacggac
     6601 gaggcgctcg cctgcctgat gtcgcatcct ttcccgggca acgtgaggga gctggagaac
     6661 atcatcgagc ggcccttcat cctgtgcagg gcgggggaga tcgaccgcgg ccatcttccg
     6721 gacggcctct gcccgaccac cgcctccgcg ggtacctccc gccttgccac gcatcggagc
     6781 ttcaagcagc tcgaggccgc cttcgtctcc agcgccctgg agcgcaacgg ctggaacaag
     6841 ctcaagacgg cacgggaact gggcatccac aagaccaccc tctttcgcaa gatgagggcc
     6901 ctggggatca gcggcgcccc tgaccccggc cgatgagtgt attcctgcaa cccctcctga
     6961 cgtcccagta gcgtttctgc accgacaatg gacggcccgg ccggtgcccg tcccgctttt
     7021 ccttctccca tagcgattta gggttcaagc ctcatttcct gggtctggca cgcatcctgc
     7081 atttcaccag acgcagatga gcaggattgc ggtagccatc tggcaaggtc acgtggcaac
     7141 gacgctcgac tttgccggca ctctcctcct cgtcgacgtc caggatggta ccatcaccgc
     7201 ccaaagcgag gtcgggctgg aggaatccgc ggttcggcat ctcgccggcc ttctgggagg
     7261 acgggggatc cagaccgtga tctgcggggc gatctctcgc ccgctcctgc ggagcctgca
     7321 agccggcggg atacgcgtcg tgccgttcgt gcggggagaa gcccaagagg tcctcgcggc
     7381 gtttctggac ggcactctgg aggacagcag gttcctgatg gcgggctgcg cgcctggcgc
     7441 acgcagaacc gtcccggaac cgaaagccgg ggtcgtgggc ggcgccatcc cggcgcctga
     7501 gaggatctgg ttgaagagga ggtctccaat gcctggaaga gacggaacgg gcggcgagca
     7561 gcgcagttcc ggattgcgcg tagcgattcc gctggatgag aaccggctgt cggcgcactt
     7621 cggtcacggc aagcgcttcg cgctgatgga cgtagacacg gaggcgaagc tggtcctgtc
     7681 gcggcaggac ttcgacgctc cggagcacgt gccgggggcc tttccccggt ggcttgccgc
     7741 caacggcgcc cagctcgtga tagcgggcac gatgggggcg aacgcccagc gtctgctcgc
     7801 ccaagccggc atccaggtgc tgatgggagc gcccgtggaa actcctgagg agctggtcgg
     7861 ggcatacctg gaagggaagc tgcgcctcga gccggcgccc tgcgatcacg gaggtggaca
     7921 tcatgatgcg gacggtagca ccggttcaca cgctcacggt gacgatcctc gtcgatgacg
     7981 acggggagag cagccggacc gagcacggcc tgtccctctg ggtggacgcg gatcggttcc
     8041 gcatcctgtt cgactcgggt tcgggaggca agctcgagga gaacgcccgg cacctgggcg
     8101 tagacctcgc ggaggccgac gcctttgccg tgagtcacgg tcaccatgac cacaccggag
     8161 gcttcgccac cgtcctctcg aagtgcggca aggcccggat ctacctgcat cccggcgcgc
     8221 tgggggagcg ctatgcccga tccagggacg ggtctatacg cgccgtgggg tgccccctga
     8281 cccctgggga ggacctggct cgacgggatc cattcctgca ctgaccagtg gggtgacgcg
     8341 cctgcacccc gcggtcttcg tgaccggcga gatccagcgg ctggcgccca cggaagcctt
     8401 gggggaccct ttctacctgg attcccggtg ctccgtcgcg gacccgctga gcgacgatca
     8461 ggcgctggtg gtcgacaccc aggatggcgc ggttgtgctc ctgggatgct cccattcggg
     8521 agtagggaac acgctcgctt ccgcgatgtc ccacctgtcc actcggaagc tccgcgcggt
     8581 ggtgggcggg atgcacctcg aggatgcgtc gctggacgcc atgacccggg tgggcaacgc
     8641 gctggaggag ttgggcgcgc tcaccatctg tccctgccac tgcacgggag acaaggcgaa
     8701 ggaatacctg cgccgcagat tccccaaggc gtacgtgcac gggtcggtag gcaccgtgct
     8761 gaagctctcc ggcgcctgac gccggaaaag gaggacgcat tgaaatctac gtccaaacac
     8821 gtgtctcact gctatcagtc ggtcgtggac aacgatcggc accacggcct ggtcctggac
     8881 ctgccgggag ccaagggagg ggatgatctg ggccccaccg cgctggagct ggcggtcatg
     8941 gcgctctccg gctgcatctc cacgatctgg gcgatcgtgg cgacgaactc caaggtctcc
     9001 tatgacaagc tcatcgtggt cgttgacgcg gacaagccgg acgatcaacc cacgacaacc
     9061 gcggcgcggg ccctggtcac cgtaagctcc cgggagcccc tggagaagct gcagcgcacc
     9121 ctggacagga ccatggccac gtgcccggtc gggaagctct acgagaaagc cgggatcgcc
     9181 atcgagacaa agctcgtgct gaacggggac tgagtcgcat cctgcgggct cgaagtgatc
     9241 agctctccct gcaacggtac ccgcgtcacg atctgggccc ctgccgagca gcggggtggt
     9301 agaggggcgg ccgcggcgga cgccccggtc tccacgagca tcacgccgcg cggcgcgccg
     9361 ccaccacggt ctcgcggccc tgccaacacc tgtcaatggt ctcaaccagg ctttggcgtt
     9421 ccataagcgc tggcctggaa acgagttaac ccctccaggg tctctatccc atcaagttaa
     9481 gttgatcggg cgtcctgacc gcgtccggga gccgattctt ccgtaattca cacccagtag
     9541 agcaattacc ccatctccgg gggagtggca cgcctcttgc ttgatgaaag tcatcaacgg
     9601 ctatcggttc gccataccgt tttggccccg accgtatggc agtagtccgc ccgagcgggg
     9661 cggacagaca ccggaggaga tcccatgaag ccgctaacag agcggtacga tgtgtccacc
     9721 gtgcacaacc agtcagagga acgcgtctac atccgcgtgg agaaggccct cgaggagcac
     9781 ccggaggtct gccggtgtcc cacgtgcgtc ctcgatctgg tggcgttcat gctgaaccgc
     9841 gtcactcctc gctattccac tggccttctt ggcgagcttc accctcgaaa gctcctggag
     9901 cgcaagatgg gactggagat cgagctttcc ctgaaggccg gaatggagcg gctgaggcgc
     9961 catccgcatc acgagagcgg aagcaccgcc ggcgcctgac gaacgacgcg agatggcctg
    10021 accgagacgg
//
LOCUS       DASRZS010000141         6828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774 k141_79329,
            whole genome shotgun sequence.
ACCESSION   DASRZS010000141 DASRZS010000000
VERSION     DASRZS010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79329"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VFH83_03540"
     CDS             complement(<1..663)
                     /locus_tag="VFH83_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485466.1"
                     /translation="MTNGEEPRPQESSGRVLDAGAPEEGAYSVASQWQLIWWKFRKHK
                     MANAAGIIIAVLYFGAIFADLLSPFLPDTRLTDYKEMPPQRIRIFDGRSLRWPFVYEV
                     QRTTDPKTFKRVYTTDPSKTYPIRLFAKGEQYKLFGVFRVRVHLFGTDGGPLALLGTD
                     MLGRDLFTRILYGSRLSLSIGLVGVFFTLVLGLLLGGLSGYLGGATDTIIQRAIDVIV
                     SIP"
     gene            complement(703..1707)
                     /locus_tag="VFH83_03545"
     CDS             complement(703..1707)
                     /locus_tag="VFH83_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485467.1"
                     /translation="MLGYIGRRLLQIIPLLVLLSILIFAIIQLPPGDFVTKQVQTLKM
                     QGTEVAQSQIDNLRNLYNLDKPMYLQYWFWIKNIVTKGDFGYSMAWNKPVTELIRERF
                     ATTFVVSILTLLLTWVVAIPIGIYSATHQYSPLDYLFTFIGFLGIAVPGFLIALVLLY
                     LVFSKTGVAITGLFSQQYIDAPLSWGKIVDGAKHIWVPVLIIGLSGTGSLIRVTRNML
                     LDELSKQYVITARSKGVPEGRLLWKYPVRIAINPLISTIGWLLPAIISGEALVSIVLN
                     MPSIGPLMLLAMLNQDMYLAGSFILVTSILTVFGTLISDILLAWFDPRIRFGTVRDGS
                     "
     gene            complement(1808..3661)
                     /locus_tag="VFH83_03550"
     CDS             complement(1808..3661)
                     /locus_tag="VFH83_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6485468.1"
                     /translation="MMRKMLLVLLALCLVATVAVAQTKYKESPMLTDLVKAGKLPPVD
                     QRLPSSPKLVNEMPASQLKYEIGKYGGTLRVAALTGRDADVFVFQNEPLVNTPGLLGN
                     EVTGNVLKSYEVSTDQKVFTFHMREGMKWSDGQPVNTEDVRYAWEDVLNNKDLTSDVP
                     SWLKAAGNPNGEPMKLQIVDTYTFKLTSSEPYGGLLLVMALEGWKGYEELIKPAHWLK
                     QFHKKYADPAALQKLIDADGQVKDWIQLHQKKDRPGTTDELTIGAPKLYPWLLVSATQ
                     QQKVYERNPYYFKVDSAGNQLPYIDKLVCTFVQDPEALSLKLVSGDVDYVREALALNK
                     MPLYKQYETNGYKVGIYTSHVTPTDIFINWTYKDPIWRKVVWDLRFRQALSYAIDRKE
                     IIDSVYFGYAQPSKIGNSEYNPAKANALLDAVGMSKKDSDGFRLGPDGKPFSIDFEVS
                     NGLWADLVPLTELVVQYWNKVGVKTTMKGVDTGLWWNDSTANNLKATVVNTQTPMWPM
                     VPKLDWWPHWAPLWTTWWYSGGKQGEEPPTDIKNFLVKLNSIYRVPVSQAPKVGADVL
                     KDIGDKLLYFVHIQNVGQPMIYNAKLGNIGPDPSAFAIAYNFSAEEFFFKQ"
     gene            3993..6632
                     /locus_tag="VFH83_03555"
     CDS             3993..6632
                     /locus_tag="VFH83_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HET6485469.1"
                     /translation="MMDTRFAEPLCSLTPDTPPTGHICPLFWQHREPPARLREEMRAM
                     RSAGIRQCILESRPHPDWLGPGWWESLDAILDEAGKLGMRVWVFDDSTYPSGRAGTLI
                     RDRHPDLMKVYLARRQIDCRGPLNGSAFRVGGWVGDGEKLEAVVAGRRRDPREAALVP
                     GSLVDLTDRVAGGLLRWDVPEGWWRVYVLVRTRNGGEEWTRDYMNPIVPAAGKAFVQE
                     VHEAHYARYGKLFGTTFAGFFTDEPRFGNKPVYDAILGNGDMVIPWSDTLLSELSEGW
                     DGSFRPALPSLWDDAGELSHRARYRFMDTVSRAFGACFSSTIGDWCRAHKVELIGHVV
                     EDNDAHARLGHGAGHFFRALQGQDMSGLDIVYSAWPGLPEGTISSPFGPWDLDFFRWG
                     IGKMAASAAHADPRKAGRVMCELFGAYGWQLGLRDMKWLTDHACARGINFLVPHAFSP
                     RFPDPDCPPHFYAGGNNPQWRLFGHWSSYAERLCHLLTGGQHRAPVALLYHAEAEWAG
                     ECELFRLPLKAMAERQIDCDVVSIDSLADPEVTRIASGSFAVNQESFRCLVVPYAQRI
                     PRRLLDVLTRLEAAKVPVFFTNGLPFGASEGDITPAELGALEANPLTAVVPTDRLGDE
                     LAARGMRDIRAEPEQPDLRTYYYRRRGMDLVFCANEDPHRTVDTLLSFEDARDPVGYD
                     ALNDLVVPVPTRRTGKTVSVRLRLDPYGSRFIVFSESPIRAAAEAPMFSEDLEPAGPV
                     AGPWRLSVAEAGETLAFTARSQPVAAGDLTLVPGLETFGGTAAYETDLTISDHGPFWL
                     DLGYVGESSEVWLDGAFLGTRICPPHLYALGSPSSGKHALRVQVTTTLARRHGDNAFD
                     RAMAQLPNGLCGPVRVMKRKAGG"
     gene            complement(6640..>6828)
                     /locus_tag="VFH83_03560"
     CDS             complement(6640..>6828)
                     /locus_tag="VFH83_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET6485470.1"
                     /translation="CVEETAREAFHLGLKTIVLSDCVSSFDPAMHAATLRNFAMKFGR
                     VMDSRSLAAEIEAAAGLR"
BASE COUNT         1131 a         2168 c         2297 g         1232 t
ORIGIN      
        1 ggggatgctg acgatcacgt cgatggcacg ctggatgatg gtgtccgtgg ccccgcccag
       61 gtagcccgag aggccgccca ggagcagccc cagcaccagg gtgaagaaca ccccgaccag
      121 gccgatggac agcgacaggc gggagccgta gaggatccgg gtgaagaggt cgcggcccag
      181 catgtccgtt cccaacaggg caagcggtcc gccgtccgtg ccgaagagat ggacccgcac
      241 ccgaaagaca ccgaagagct tgtactgctc ccccttggcg aagaggcgga tggggtaggt
      301 cttgctgggg tccgtcgtgt agacccgctt gaacgtcttg ggatccgtgg tgcgctggac
      361 ctcgtagacg aacggccagc ggaggctcct gccgtcgaag atccggatgc gctggggcgg
      421 catctccttg tagtccgtga gccgggtgtc cggaaggaac gggctcagga ggtccgcgaa
      481 gatggcgcca aagtacagca cggcgatgat gatccccgcc gcgttcgcca tcttgtgctt
      541 tcggaacttc caccagatga gctgccactg ggaggcgacg gaatacgccc cctcctccgg
      601 cgcaccggcg tccaggacgc ggcctgagga ctcttgcgga cggggctctt cgccattcgt
      661 cacgatgcga ccctcctgcg cggggatgcc gaccgtcgtc ggctagctgc cgtcccgcac
      721 ggtcccgaac cggatgcggg gatcgaacca ggccagcaga atatcggaga tgagggtacc
      781 gaagacggtc aggatgctgg tcaccaggat gaagcttccg gccaggtaca tgtcctggtt
      841 cagcatggcc agcagcatga gcggtccgat cgaaggcatg ttgagcacga tggacacgag
      901 cgcctctccc gagatgatgg cgggcagcag ccatccgatg gtgctgatga gcgggttgat
      961 ggcgatccgc acgggatact tccagagcag ccgcccctcc ggcactcctt tggatcgagc
     1021 ggtgatcacg tactgcttgg agagctcgtc caggagcatg ttgcgggtca cccggatgag
     1081 cgagcccgtg ccggagagcc cgatgatcag caccggcacc cagatatgct tggctccgtc
     1141 cacgatcttt ccccagctca ggggggcgtc gatgtattgc tgggagaaca gacccgtgat
     1201 ggcgacgccc gtcttggaga agaccaggta cagcagcacc agggctatga ggaagccggg
     1261 taccgcgatc cccaggaagc cgatgaaggt gaacagatag tcgagcggcg agtactggtg
     1321 cgtggcggag tagatcccaa tggggatggc gacgacccag gtcaggagga gtgtcaggat
     1381 ggagaccacg aaggtggtgg caaaccgctc acggatcagc tccgtcacgg gcttgttcca
     1441 cgccatggaa tacccgaagt cccccttggt gacgatgttc ttgatccaga accaatactg
     1501 gaggtacatc ggcttgtcca ggttgtagag gttgcgcagg ttgtcgatct ggctctgcgc
     1561 cacctcggtg ccctgcatct tgagggtctg cacctgcttg gtgacgaagt ctcccggcgg
     1621 gagctggatg atggcgaaga tcaggatgga gagaaggacc agcagcggga tgatctgaag
     1681 caggcgacgc ccaatataac ccagcatggc ctttccttcc cgggtgccgc ccgggtccgg
     1741 ctcttgggcc ggaatggcag gcggcggggg aacccgccgc ctgctcacgt ccgagcgttc
     1801 cggagaccta ctgcttgaag aagaactctt cggccgagaa gttgtaggcg atggcgaacg
     1861 cgctcgggtc gggcccgatg ttgcccagct tcgcgttgta gatcatgggc tgcccgacgt
     1921 tctggatatg cacgaagtac aggagcttgt ccccgatgtc cttcaggacg tcggcaccga
     1981 ccttgggggc ctggctcacg ggcacccggt agatcgagtt cagcttgacc aggaagttct
     2041 tgatgtcggt gggcggttcc tcaccctgct tgccgccgga gtaccaccag gtggtccaca
     2101 ggggcgccca gtgcggccac cagtccagct tgggcaccat gggccacatc ggcgtctgcg
     2161 tgttgaccac ggtggccttc agattgttgg cggtggagtc gttccaccac aggccggtgt
     2221 ccacgccctt catggtcgtc ttgacgccga ccttgttcca gtactggacc acgagctccg
     2281 tcagcgggac aaggtccgcc cagagtccgt tggacacctc gaagtcgatg ctgaagggtt
     2341 tgccgtcggg ccccagacgg aagccgtccg agtccttctt gctcatcccc acggcgtcca
     2401 ggagcgcgtt ggctttggcg gggttgtact ccgagttgcc gatcttggac ggctgggcgt
     2461 agccgaagta cacggagtcg atgatctcct tgcggtcgat ggcgtagctc agcgcctggc
     2521 ggaagcgcag gtcccagacc accttgcgcc agatcggatc cttgtacgtc cagttgatga
     2581 agatgtccgt cggcgtgaca tggctggtgt agatgcccac cttgtagccg ttggtctcgt
     2641 actgcttgta cagcggcatc ttgttgagcg ccagggcttc ccgcacgtag tccacgtcac
     2701 ccgagaccag cttcagggac agcgcctcgg gatcctggac gaacgtgcac acgagcttgt
     2761 cgatgtacgg gagctggttg cccgcggagt ccaccttgaa gtagtacggg ttgcgctcgt
     2821 agaccttctg ctgctgggtc gcggacacga gcagccaggg gtaaagcttg ggcgcgccga
     2881 tggtcagctc gtccgtggtc cccgggcggt ccttcttctg gtggagctgg atccagtcct
     2941 tcacctggcc gtccgcgtcg atgagcttct gcagggcggc gggatccgcg tacttcttgt
     3001 ggaactgctt cagccaatgc gccggtttga tcagctcttc atagcccttc cagccttcca
     3061 acgccatgac cagcaggagg ccgccgtacg gctcgctgga ggtcagcttg aaggtgtagg
     3121 tgtccacgat ctggagcttc atgggctcac cgttggggtt gcccgcggcc ttgagccagg
     3181 acggcacgtc cgaggtgagg tccttgttgt tgaggacgtc ttcccacgcg tagcgcacgt
     3241 cctcggtgtt cacgggctgc ccgtcggacc acttcatgcc ctcccgcatg tggaaggtga
     3301 agaccttctg gtccgtggag acctcgtagc tcttcagcac gttgcccgtg acctcgttgc
     3361 ccaggaggcc cggcgtgttg accaggggtt cgttctggaa gacgaagacg tccgcgtccc
     3421 ggcccgtgag cgcggccacg cggagcgtgc cgccgtactt cccgatctcg tacttgagct
     3481 ggcttgccgg catctcgttc acgagcttgg ggctgctggg cagccgctga tccaccggcg
     3541 gaagcttccc ggccttcacc aggtcggtca gcatgggcga ctccttgtac tttgtctgcg
     3601 cgaccgccac cgtcgcgacc aggcacagtg caaggagaac caaaagcatc ttcctcatca
     3661 tctggacctc cttgatctag ctgatgccgt ctttggattg gatcgacgat ccacttgaag
     3721 ggccatccgt agggattggg acagtgacat cacgcgtgtg cacggtggaa acggatcgta
     3781 gcacgggccg gatgcgaagt caagttttca gcgcctcggt caccgctgcg gagaacgcgc
     3841 ggaggacggg cgcgtgccgg gcgggtcgga cgcggccgcc ccggtatcga tgcaccgcac
     3901 ggccttcgag ccgtacgccg cgcgcgcccg ttggacattt cactcacgat ccccggattg
     3961 tgacacgacg ccggctccgg tacaatcccg ccatgatgga cacgcgattc gcggaacccc
     4021 tctgctccct cacgccggac acccctccca ccgggcacat ctgtccgctc ttctggcagc
     4081 accgcgagcc tccggcccgg ctgcgggagg agatgcgcgc catgcggtcc gccgggatcc
     4141 gtcagtgcat cctggaatcc cggcctcacc ccgactggct cggacccgga tggtgggaga
     4201 gcctggatgc cattctggac gaggcgggca agctgggcat gcgcgtgtgg gtcttcgacg
     4261 attccacgta tcccagcggc cgcgccggca ccctgatccg ggatcggcac cccgacctca
     4321 tgaaggtcta cctggcgcgc cgacagatcg attgccgggg gccgctgaac ggctcggcgt
     4381 tccgggtcgg tggctgggtc ggcgacgggg agaagctcga ggcggtcgtg gccggtcggc
     4441 gccgggatcc gcgggaagcg gcgctggttc ccggctccct ggtggacctg acggatcgag
     4501 tggccggcgg actcctgcga tgggacgtcc cggaaggctg gtggcgggtc tacgtcctgg
     4561 tgcggacccg aaacggcgga gaggagtgga cacgggacta catgaacccc atcgtgccgg
     4621 cggccggcaa ggcattcgtc caggaagtcc acgaggcgca ctacgctcga tacggcaagc
     4681 tcttcggcac caccttcgcg gggttcttca ccgacgagcc gcgcttcggg aacaagccgg
     4741 tatacgacgc catcctgggc aacggggata tggtcatccc gtggagcgac accctcctct
     4801 ccgagctttc ggagggctgg gacggaagct tccgccctgc gctcccgtcg ctgtgggacg
     4861 atgccggcga gctctcccac cgggcgcggt accgattcat ggacaccgtg agccgggcct
     4921 ttggcgcatg cttctcctcg accatcggag actggtgccg cgcccacaag gtcgagctca
     4981 tcggccatgt cgtggaagac aacgacgccc atgcccggct gggtcacggg gcgggtcatt
     5041 tcttccgcgc ccttcaggga caggacatgt cggggctgga catcgtctac tcggcatggc
     5101 ccggcctgcc ggagggaacg atctcctcac cgttcggccc gtgggacctc gacttcttcc
     5161 ggtggggcat cggcaagatg gctgcctccg cggcgcacgc ggacccccga aaggccggcc
     5221 gcgtgatgtg tgagctcttt ggcgcctacg gatggcagct cgggctgagg gacatgaagt
     5281 ggctcacgga ccatgcctgc gcgcgcggca tcaacttcct ggtgccgcac gcgttctccc
     5341 cgcggttccc ggacccggac tgtccgccgc acttctacgc cggagggaac aacccgcagt
     5401 ggcggctctt cgggcactgg tcttcctatg cggagcggct gtgccacctg ctcaccggcg
     5461 gacagcaccg ggcgccagtg gcgctcctct atcacgcgga ggccgaatgg gcgggggagt
     5521 gcgagctctt ccgcctgccg ctcaaagcca tggcggagcg ccagatcgac tgcgacgtgg
     5581 tcagcatcga ttcgctcgcg gaccccgagg tcacccggat cgcaagcgga tccttcgccg
     5641 tgaaccagga gagtttccgg tgcctggtcg tcccctatgc ccagaggatc ccgcgccgat
     5701 tgctggacgt gctgacgagg ctggaagcgg cgaaggtccc ggtgttcttc acgaacggtc
     5761 tccccttcgg cgccagcgaa ggggatatca ccccggcgga gctcggagcc ctcgaggcga
     5821 accccctgac cgccgttgtt cccacggacc gtctcggcga tgagctggca gcccggggaa
     5881 tgcgggacat ccgcgccgag ccggaacagc ccgacctgcg gacctattac tatcgtagaa
     5941 ggggcatgga cctcgtgttc tgcgccaacg aggatcccca tcgaaccgtc gacacgttgt
     6001 tgtccttcga ggatgcgcgt gaccccgtgg gctacgacgc cttgaacgat ctggtggttc
     6061 cggtgccgac ccgtcgcacc ggcaagacgg tctccgtgcg gctccgtctg gacccatacg
     6121 gctcccggtt catcgtgttc tccgagagcc cgatccgcgc cgccgcggag gcgccgatgt
     6181 tctcggagga cctggaaccg gccggacccg tggccggccc ctggaggcta tcggtggcgg
     6241 aggccgggga gacgctggcg ttcacggcgc gctcgcagcc ggtggcggcc ggggacctta
     6301 ccctggtccc ggggctggag accttcggcg ggaccgccgc atacgagacg gatctcacga
     6361 tctccgatca tggaccgttc tggctggatc tcggctacgt cggggaatcg agcgaagtgt
     6421 ggctggacgg cgcgttcctg ggtacccgca tctgccctcc tcacctctac gcgctcggtt
     6481 ctccctcctc ggggaagcat gcgctgagag tccaggtgac caccacgctg gcgcgcaggc
     6541 acggggacaa cgcctttgac cgcgccatgg cccagcttcc gaacggcctc tgcggcccgg
     6601 tgcgggtgat gaagcggaag gcaggcgggt aggggcggcc tatcgcaggc ccgcggccgc
     6661 ctcgatctcc gcggccagcg agcgtgaatc catcaccctg ccgaacttca tggcgaagtt
     6721 cctgagcgtg gccgcgtgca tcgccgggtc gaaggacgac acgcagtcgc tcagcacgat
     6781 ggtcttgaga cccaggtgga aggcctcccg ggccgtctct tctacgca
//
LOCUS       DASRZS010000142         5206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4313651, whole genome shotgun sequence.
ACCESSION   DASRZS010000142 DASRZS010000000
VERSION     DASRZS010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4313651"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VFH83_03565"
     CDS             <1..917
                     /locus_tag="VFH83_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6485471.1"
                     /translation="RRTRAVILVHLAGVPADVKPIARLCKKRGIALIEDCAQSWGAKV
                     DGRWVGTFGQIGAFSFNDYKHVSAGEGGLIVTDDPKLYATAWRGIDKCYDRLTGKRDV
                     YFVAPNYRITELQSAVGIAQLGKVASICSTRHRLGERLSRGLAGIPGIRPHAVAPGGY
                     ATYWYYLIGLEPEVLHVDAETFGRAMNAEGIAGSGRFYMEPVHLAYSYLKGKSAFFHS
                     HYPFDLGRNEVSYERGICPNAEALLDRVYYFPLNEWLTDREIDDTIEAVRKVAAFYRE
                     RGMTARGRIAANKGRRVAAGKKAGARRR"
     gene            complement(982..1272)
                     /locus_tag="VFH83_03570"
     CDS             complement(982..1272)
                     /locus_tag="VFH83_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485472.1"
                     /translation="MMDPISGSTAGSMAPKPGALTPAPAPSPQKLSGTPSSGALSSGT
                     PSVAASSKEDSVELSLTAQIRNLKLQGQSLNQIAIALGLDTKTVESYLGETV"
     gene            complement(1358..2359)
                     /locus_tag="VFH83_03575"
     CDS             complement(1358..2359)
                     /locus_tag="VFH83_03575"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET6485473.1"
                     /translation="MAGRGPEVEIPWYRTRIERETLRSLTRRSDARGFLQVVPHLALV
                     ILSGAAAFYAAAWWPWWTLLPILLAHGSLYAFLANGFHELTHGTVFKTRWLNGVFLWI
                     FSFLTWSSPVLYKASHTRHHLYTLHPPHDQEVVLPMRLTLRGFLLSAVIDPLGFYGTI
                     WNTVRVAFGRLKGPWENALFPQTEPVERRRLFRWARITLAGHAIIVAFSVVTGWWLVA
                     VLTTGAAFYGRWVFFLNTYSQHTGLQDNVPDFRRCTRTIVHTPVLQYLYFHMNYHAEH
                     HMYASVPCYNLRRLRRVIGHDMPKAKGMIGSWRDIIAILRRQRSDPTYQYEYELPRS"
     gene            complement(2419..4287)
                     /locus_tag="VFH83_03580"
     CDS             complement(2419..4287)
                     /locus_tag="VFH83_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485474.1"
                     /translation="MRRKRPDPQAARPRETADRPTDPSEPGRTPSALKSVWMILRASF
                     AAAPGATAATLVLHPIGYLEAPLVALGLRFATNGAVAGDPRLLGLAGVLLVTALCVFH
                     IVNHTAWQLSLGLEDRVAMVLDRRIGELVSGTPGLAAFERPQLADRLEMLRERGWVLG
                     RAMYLLPIELGEIARAAVTFVLLGTVHPALLLLPVFALPSILMDARSERRAREAEEGS
                     VDAIRRSRHLYELATTAGPGRELRVFALGPEILSRHRKEWQRIHAARISALKESTGRS
                     ILGWICYACGFVAAAAFVGWRVWSGAASVGDLVLTLALAAQVNGEARVAVEIVGWVRW
                     VASIGGHLLWLEQQRDRDAHARAGTEMPPGCLRDGIAFEHVSFRYPGTDRWVVRDVSL
                     HLAAGATVAIVGENGAGKTTLVKLLCRFYEPTEGTITVDGRRLPELEREQWLKRVTAS
                     FQDFCRFELLLREAVGIGDLAPAEVDRIRSAVRRADATDVLAALPHDLETQLGSTWPS
                     GVDLSTGQWQKVALARTMMRREPLLLVLDEPTASLDPQTEYSLFERQAQASRGAPGAI
                     TLIVSHRFSTVARADRIVVMREGAVVEQGTHAELMARPGLYAELYSLQARAYRRKT"
     gene            complement(4268..>5206)
                     /locus_tag="VFH83_03585"
     CDS             complement(4268..>5206)
                     /locus_tag="VFH83_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485475.1"
                     /translation="VGAKDWGRTAAWWTRSLYGSDSVTHALALSSFTSSASGIRSGAG
                     DCAGLPRHGVTLDGLAFRYPGATVDLFSDLRLEIPAGRSLAIVGRNGAGKTTIVKLLA
                     RFYDPSGGRILVDGVDLRSLDLAEWRKRLAVIFQDFTHYELAARENIGFGCPKLLSDE
                     RTLAAAASRAEALTLVESLPRGWDTILSRAYEGGAELSGGEWQRIALARALAAIEGGA
                     TLLILDEPSANLDVRAEAALFDRFLDLTRGLTTILISHRLSTVRHADEICVIDEGRVL
                     ERGAHEALLESGGVYAGLFRLQAQSFLEEGRAAEET"
BASE COUNT          894 a         1758 c         1766 g          788 t
ORIGIN      
        1 cccggcgcac gcgggcggtc atcctggtcc acctggccgg cgtcccggcg gacgtgaagc
       61 caatcgcgcg cctctgcaag aagcggggga tcgccttgat cgaggattgc gcccagtcct
      121 ggggcgcaaa ggtggacggc cgctgggtcg ggaccttcgg ccagatcggc gccttcagct
      181 tcaacgatta caaacacgtc agcgccgggg aaggcgggct catcgtcacg gacgatccga
      241 agctctacgc caccgcctgg cgcggcatcg acaagtgcta cgaccggctg accgggaaac
      301 gggacgtcta cttcgtggcg ccaaactacc gtatcacgga gcttcagagc gccgtgggga
      361 tcgcgcagct cggcaaggtt gcgtccatct gctccacgcg gcaccgcctg ggggaacggc
      421 tctcccgggg cctggccggc attcccggca tccgcccgca cgcggtggcg ccgggcggct
      481 acgccacgta ttggtactat ctgatcgggc tggaaccgga ggtgctccac gtggacgcgg
      541 agacctttgg ccgcgccatg aacgcggagg ggatcgcggg cagcggccgg ttctacatgg
      601 agccggtcca cctggcgtat tcctacctca aaggaaagag cgccttcttc cactcccact
      661 acccgttcga cctcggacgc aacgaggtga gctacgagcg ggggatctgt ccgaacgccg
      721 aagcgctcct ggaccgcgtg tactacttcc ctctgaacga gtggctcacg gaccgtgaga
      781 tcgacgacac catcgaggcc gtgcggaagg tggccgcatt ctaccgcgag cgggggatga
      841 cggcgcgggg gaggatcgcg gcgaacaagg ggcgcagggt cgctgcgggg aagaaggcgg
      901 gggcccgccg ccggtaggca gggatgcgag gattgcgtcc cccgcgcctt ttcgccgagg
      961 gacgcgtctc ctccttttgg actagaccgt ctctccgagg taggactcca cggtcttggt
     1021 gtccagaccg agagcgatgg caatctggtt gaggctctga ccctgcagct tgaggttgcg
     1081 gatctgggcc gtgagcgaaa gctctacgct atcctccttg ctggacgccg ccacgcttgg
     1141 cgtccccgag ctgagcgctc ctgagcttgg cgtccccgac agcttctgcg gggaaggggc
     1201 cggtgccggt gtcagggctc ccggcttggg agccatcgag ccggccgtgc tgcctgaaat
     1261 cggatccatc atacgagctc cttcgagaga ccgtccttgg tctctctcag tcatcctatc
     1321 ggcctcgtga ctcttgaact tgcgcggacc cgttccctca ggagcggggt agctcgtact
     1381 cgtactggta ggtggggtcg gagcgctgcc gcctcaggat ggcgatgatg tcccgccagg
     1441 agccgatcat ccccttggcc ttcggcatgt catggccgat cacccgtcgc agccgtctca
     1501 ggttgtagca gggcaccgag gcatacatgt ggtgctcggc atgatagttc atgtggaagt
     1561 acaggtactg gagcacgggg gtatgcacga tcgtgcgggt gcaccggcgg aagtccggca
     1621 cgttgtcctg gagcccggtg tgctgggagt acgtgttgag gaagaagacc caccggccgt
     1681 agaaggcggc cccggtcgtc agcaccgcca ccagccacca gccggtcacg accgagaacg
     1741 ccacgatgat ggcgtgtccc gccagggtga tcctggccca gcggaagaga cgtctccgct
     1801 cgacaggctc ggtctggggg aacagggcgt tctcccacgg ccccttcagc ctcccgaagg
     1861 ccacgcgcac cgtgttccag atcgttccgt agaatcccag cgggtcgatg acggcgctca
     1921 ggaggaagcc gcgaagggtc aggcgcatcg gcagcacgac ctcctggtca tgcggcgggt
     1981 gcagggtgta caggtggtgc cgggtatggc tggccttgta gagcaccggg ctggaccagg
     2041 tcagaaagct gaagatccag aggaagaccc cgttcagcca ccgggtcttg aagaccgtgc
     2101 cgtgggtcag ctcgtgaaag ccgtttgcca ggaacgcata cagggatcca tgcgccagga
     2161 ggatgggcag cagagtccac cagggccacc atgccgcggc atagaacgcc gcagcgccgg
     2221 ataggatcac cagcgcaagg tgggggacca cctggaggaa cccccgggcg tcgctccgcc
     2281 gggtgaggga gcgcaacgtt tcccgctcga tccgggtccg ataccagggg atctcgacct
     2341 caggtccgcg accagccacg acgtatcctc cggacgggga gcgtccaccc gccatcatag
     2401 tcccacgccg ccgacggctc aagtcttgcg acgatacgcc cgggcctgga gggagtacag
     2461 ctccgcgtac agccccggtc gcgccatgag ctcggcgtgg gtgccctgct ccactaccgc
     2521 cccctccctc atcacgacga tccggtccgc ccgcgcgacg gtggagaacc ggtgactgac
     2581 gatcagggtg atcgccccgg gcgcgccacg ggaggcctga gcctggcgct cgaacagcga
     2641 gtattcggtc tggggatcca ggctcgccgt aggctcatcc agaacgagga ggaggggctc
     2701 ccgacgcatc atcgtccgag ccagggcgac cttctgccac tgacccgtgg agaggtccac
     2761 cccagagggc caggtcgatc cgagctgggt ctcaagatcg tggggcagcg cggcaaggac
     2821 atccgtggcg tccgctcgcc gtaccgcgga acggatacgg tccacctccg ctggtgccag
     2881 gtctccgata ccgaccgctt ctcggagcag aagctcgaac cggcagaagt cctggaacga
     2941 ggcggtgacc cgcttcagcc attgctcccg ctccagctcc ggtaggcgac gaccgtctac
     3001 ggtaatggtt ccctcggtgg gctcatagaa tcgacacagg agcttgacca gcgtcgtctt
     3061 gccggcgccg ttctctccca cgatcgctac cgtggcgccc gcggccagat gcaggctcac
     3121 gtcccgcacg acccaccgat ccgtcccggg gtaccggaag gagacgtgct cgaaggctat
     3181 cccgtctcga aggcaaccag gaggcatctc ggtcccggca cgcgcgtgcg cgtcccggtc
     3241 ccgctgctgc tccagccaca ggaggtgtcc cccgatgctc gccacccaac gcacccatcc
     3301 cacgatctcc acggctaccc gggcctcccc gtttacctgg gcagccaggg ccagcgtgag
     3361 cacgaggtcc ccgacgctcg cggcgccgga ccagactcgc catccgacga acgccgcggc
     3421 ggcaacgaat ccgcatgcgt agcagatcca gccgaggatg gatctcccgg tcgactcctt
     3481 caaagcggag atccgggcgg cgtggatcct ctgccattcc ttgcgatgtc gagagaggat
     3541 ctcgggaccc agagcgaaaa cccgcagctc ccgtcccggg cctgccgtgg tcgccagctc
     3601 gtagaggtgg cgtgagcggc ggatggcgtc cacgctcccc tcttcagcct cccgcgccct
     3661 ccgctcggac cgggcgtcca tcaggatcga tggaagggcg aagaccggga gcaggagcaa
     3721 ggcagggtga acggtcccca ggagcacgaa ggtgaccgcg gctctggcga tctctcccag
     3781 ctcgatcggc aggaggtaca tggcgcggcc cagcacccat ccccgctccc gcagcatctc
     3841 cagccggtcg gcgagctgcg gccgctcgaa cgccgcgagc ccgggtgtgc cagagacgag
     3901 ctccccgatg cgccggtcca gcaccatcgc cacccggtcc tccaacccga ggctgagctg
     3961 ccaggcggtg tgattcacga tgtgaaagac gcagagcgcg gtgaccagaa ggacgccggc
     4021 cagtcccagg aggcgcgggt cgccggccac ggcaccgttg gtcgcgaagc gaagcccgag
     4081 cgccacaagg ggagcctcca ggtagccgat ggggtgcagc acgagagtgg cggcggtggc
     4141 cccgggcgcc gcggcaaagg aggcccgcag gatcatccag acggacttca gggcggacgg
     4201 cgttcgcccg ggttccgagg gatccgtcgg tcgatccgcg gtctcgcgcg gtcgcgctgc
     4261 ctgcgggtca ggtctcttcc gccgcacgac cttcctccag gaagctctgg gcctggagtc
     4321 gaaagagccc ggcgtatacg ccgccgctct ccaggagcgc ctcgtgcgct cctcgctcca
     4381 acacacggcc ctcgtcgatc acgcagatct catccgcgtg acggacggtc gacaggcggt
     4441 gagagatcag gatcgtggtg aggccgcgcg tgaggtccag gaaccggtcg aagagcgcgg
     4501 cctccgcgcg gacatccagg ttcgcggaag gctcatccag gatgagcagc gtcgccccgc
     4561 cctcgatggc ggccagtgcc cgcgccaacg cgatccgctg ccactctccc ccggaaagct
     4621 cggcgccccc ttcgtaggca cgcgacagga tcgtgtccca gccgcgcggg agggactcga
     4681 cgagggtgag cgcttccgcg cgcgaagcgg cggccgccag cgtccgctcg tcggagagga
     4741 gcttcggaca cccgaagccg atgttctctc tcgcggccag ctcgtagtgc gtgaagtcct
     4801 ggaagatcac ggccagccgc ttgcgccatt ccgccagatc gagggacctc agatcgacgc
     4861 catccaccag gatgcggccg ccactcggat cgtagaaccg tgccaggagc ttgacgatcg
     4921 tggtcttgcc ggcgccgttt cgccccacga tggccaggga tcggccggcg ggtatctcga
     4981 gacgcagatc ggaaaacagg tccaccgtgg cgcccggata tcggaacgcg agcccgtcga
     5041 gagtgacccc atggcgcgga aggcccgcgc aatccccggc gccgctgcgg atgcccgagg
     5101 ccgagctcgt gaaggaggaa agcgccaggg cgtgcgtgac ggagtccgag ccgtacaggc
     5161 tgcgggtcca ccacgccgcg gtgcgacccc agtccttggc gccgac
//
LOCUS       DASRZS010000143         6633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1615755, whole genome shotgun sequence.
ACCESSION   DASRZS010000143 DASRZS010000000
VERSION     DASRZS010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615755"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..174
                     /locus_tag="VFH83_03590"
     CDS             16..174
                     /locus_tag="VFH83_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485476.1"
                     /translation="MPFGTADQVRAEVTERKRILGRSGGYILGPSHAIQDGTPPENIV
                     ALFDTAAG"
     gene            188..1165
                     /locus_tag="VFH83_03595"
     CDS             188..1165
                     /locus_tag="VFH83_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET6485477.1"
                     /translation="MKIGLTYDLRSEWLKQGYNELETAELDREETVQGVELAIQAAGH
                     STERIGNYRQLFQALSAGKRWDLVFNFCEGMFGLAREALVPAILDAYRIPYTFSDPAV
                     LAVSLQKALTKRVVRDAGVPTPDFHVVESPADLSAVALRYPLFAKPIAEGTGKGITPN
                     SKITSPTELERVCSELLKTYRQPVLVEEYLPGREFTTGIIGTGPKAEAIGTMEVILLD
                     TADANAYTYLNKEYCDDRVRYELATGSVGEECARVALGAWRALGARDAGRVDLRMDAG
                     GRVSFIEANPLAGLHPIHSDLPIICGLVGVSFHELIRRVIESATSRITP"
     gene            complement(1162..2052)
                     /locus_tag="VFH83_03600"
     CDS             complement(1162..2052)
                     /locus_tag="VFH83_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485478.1"
                     /translation="MEDNVGFLEFSDNEFLLSVARALADLEPKFIGVSQVEHPTAAPA
                     RVIVDRLSFCDPFLRSVVRYWGTLGARIINSPFFSFDKLAELALYNELRIGHPRTVLL
                     PGRNRMENLSGVVAPPDWGAVEERIGFPCILKPVDGYAWQDVYRVESPSALRALYGSL
                     SERRVLLVQELIRYTGYYRAFCVGAREVLIVGWKPLPFDRGEYSVVDPVGLGDLGPFI
                     HGKTAELNGKLGLDFNTVEWCVTTDGRPVVIDSNNDVPDVRKEKLPASCYDWIVERFA
                     ALVRRAVAERGEGATGTGPA"
     gene            complement(2083..2544)
                     /locus_tag="VFH83_03605"
     CDS             complement(2083..2544)
                     /locus_tag="VFH83_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485479.1"
                     /translation="MRKAVICAALILLCACGAFAAAGWALGGEGGFYIDGAGGLPASG
                     MLVFHLPQVPLMFGVGVAGSPLAIGFTADYWALKGSFNRFLSGYGGFGGYATFGAPFT
                     AGVRFPFGLQIWPIPQSLEVFLEVAPAVGLSLVPTGFDWHIQAALGLRIWF"
     gene            2703..4496
                     /gene="iorA"
                     /locus_tag="VFH83_03610"
     CDS             2703..4496
                     /gene="iorA"
                     /locus_tag="VFH83_03610"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HET6485480.1"
                     /translation="MTNAESSRALLSGNEAIARGAWEAGVTVATGYPGTPSTEILEAV
                     KASYQDIYCEWSPNEKVALEVAAGASLAGARVIVTMKHVGLNVAADPLLTLAYIGTVG
                     GLVICVADDPGMHSSQNEQDTRHYARFAKVPILEPSDSQEAKDFLALGLEISERHGTP
                     VLLRSTTRVSHSSGSVQLGERKPVPASDGFKKDPKRFVPIPMNARPMRDRLEVRLQAL
                     AGESSASGANRIEWGDRKVGVIAEGIAYQYVRETMPDASVLKIGWVWPFPDPIIREFA
                     AGVERLLVVEELDPILEEHIRSLGIACEGKSKVPAIGELSPDRLGAALGLTMPASRPA
                     AADLPARPPVLCPACPHRGIFYALGRLDVAVTGDIGCYSLGVFPPLKRIDTILCMGAG
                     ISMAHGMQVVHARTSTKEHPRQKVVGIVGDSTFFHSGITGLLDISYNLGAATVIVVDN
                     RTTAMTGHQEHPGTGKTIRGEPTVEASIEDFGRACGMKNIATVDPYDLDQTLKVLRTA
                     VDSDEPWLIVSRSPCPLYTKERVGPARQVDQELCRKCKACLKLGCPAIEWVNDEIHVN
                     ELLCGGCALCEKVCKDGAFALAKAAGACADA"
     gene            4489..5076
                     /locus_tag="VFH83_03615"
     CDS             4489..5076
                     /locus_tag="VFH83_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HET6485481.1"
                     /translation="MRETTSMVLVGVGGQGILLATQVAARAALIAGMDVKTNEVHGMA
                     QRGGSVVAQVRYGRKVYSPLVPDGSADALLSLERIEALRFASFLAPGGLAVVSRQAIV
                     PVTVSSGQATYPSDAEERLRAEFPRLAYIDAVAVAEKVCDIRVANMVLLGALSTALSL
                     PLECWSRAIAQCVKPKFVEMNERAFQEGRSRAPTS"
     gene            complement(5086..5562)
                     /locus_tag="VFH83_03620"
     CDS             complement(5086..5562)
                     /locus_tag="VFH83_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485482.1"
                     /translation="MGGSRTLRTDGLETPRVDGAVRVEGRLRNLLVLDGAVFLVCVLA
                     LYVLYRVPLLLSPLAVALLILGAAALLVLDAVLWTRGGIRSIELEPETLTMYRGRELR
                     ESRLDRRSVISVRIKRRLGRGSVTIVLATGNRIRIRDDPFPAAEFHRLVEALRRWG"
     gene            5667..>6633
                     /gene="carA"
                     /locus_tag="VFH83_03625"
     CDS             5667..>6633
                     /gene="carA"
                     /locus_tag="VFH83_03625"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET6485483.1"
                     /translation="MTKAVLALEDGAFFEGEALGAPGEAFGEVVFNTSITGYQEVLTD
                     PSYRGQIVTMTYPLIGNYGMVPEDDESDRPWLSGFVVREASAIRSNWRSTVGLADWLS
                     QLGVVGIHRIDTRALTRHLRIEGAKHGVISTVDFDRASLASKLQASPRIVGRDLVREV
                     SCEEPYRWAEPLPVPRTPAAGAPVPRTHALPTDPRRHVVVLDCGVKRNILRHLVSAGC
                     AVTVVPATTPAAQILALNPDGLLLSNGPGDPEPVTYVVETARSLLGRLPIFGICLGQQ
                     MLGLAMGGKTYKLKFGHHGGNHPVKDLATGRIAITTQNHGFNVDLE"
BASE COUNT         1164 a         2210 c         2206 g         1053 t
ORIGIN      
        1 agcgtccaga agacgctgcc cttcgggacg gcggaccagg tacgggcaga ggtgacggag
       61 cgcaagcgga tcctaggacg aagcggtggg tacatcctgg gcccgtccca cgcgatccag
      121 gacgggacgc cgcccgagaa catcgtcgcc ctgttcgaca cggcggcggg ctgaccagga
      181 gcatcggatg aagattggcc tcacgtacga ccttcggtcg gaatggctga agcagggcta
      241 caacgagctg gagaccgctg agctggaccg ggaagagacc gtccagggcg tggagctcgc
      301 catccaggcc gcgggccact ccacggagcg gatcggaaac taccgtcagc tcttccaggc
      361 cctctcggcg ggcaagcggt gggacctggt cttcaacttc tgtgaaggga tgttcggcct
      421 ggcccgagag gctctggtgc ccgcgatcct ggatgcctac cggatcccgt ataccttttc
      481 ggatcccgcg gtgctcgccg tctcccttca gaaggccctg accaagcgcg tggttcggga
      541 tgccggtgtg cccacgccgg attttcacgt ggtggagagc ccggcggacc tttccgcggt
      601 ggcgcttagg taccctctct tcgcaaagcc gatcgccgag ggcaccggga aggggattac
      661 acccaactcc aagatcacga gccccaccga gctcgagcgg gtgtgctcgg agctgctgaa
      721 gacctatcgg cagccggtgc tggtggagga gtacctgccg gggcgcgagt tcacgaccgg
      781 aatcatcggc acggggccca aggccgaggc catcggcacc atggaggtga tcctgctcga
      841 cacggcggat gccaacgcgt acacgtacct gaacaaggag tactgcgacg accgggtgcg
      901 ctatgagctg gcgaccggtt ccgtgggaga ggagtgcgcg cgagtggcgc tcggcgcgtg
      961 gcgggcacta ggcgcccggg acgccggacg cgtggacctg cggatggacg ctggcggacg
     1021 ggtgagcttc atagaggcca accccctcgc ggggctccac cccatccact ccgacctccc
     1081 gatcatctgt ggtctggtag gcgtatcctt ccacgagctg atccgccgcg tcatcgagtc
     1141 cgcgacgtcg aggatcaccc cctaggccgg accggtcccg gtcgcgcctt ccccccgttc
     1201 agcaaccgcc cggcgaacca gcgccgcaaa ccgctccacg atccagtcgt agcagctcgc
     1261 cggaagcttc tctttcctca cgtccggcac atcgttgttg gagtcgatga ccacggggcg
     1321 gccgtccgtg gtgacgcacc actccaccgt gttgaagtcc agacccagct tcccgttgag
     1381 ctccgcggtc ttcccgtgga tgaacggccc gaggtccccg agccccaccg gatcaacgac
     1441 ggagtactcc ccgcgatcga aaggcagcgg tttccacccg acgatcagga cctcgcgggc
     1501 gccgacgcaa aatgcccgat agtagcccgt gtaccggatc agctcctgga ccagcaggac
     1561 ccgtctctcg gagagggagc catacagtgc gcgcagggca gacggactct ccacccggta
     1621 gacgtcctgc caggcgtagc cgtccaccgg cttgaggatg cacgggaaac cgatccgctc
     1681 ctctaccgct ccccaatccg gaggcgccac caccccggag aggttttcca tccggttcct
     1741 gcccggcagg agcacggtgc gagggtggcc gatcctcagc tcgttgtaga gggccaactc
     1801 ggcgagcttg tcgaaggaga aaaacgggct attgatgatc cgcgccccga gagtgcccca
     1861 gtaacggacg acggaccgta gaaacgggtc gcagaagctc agccggtcca cgatcacccg
     1921 ggcgggggcc gccgtcgggt gttcgacctg ggagactccg atgaacttcg gctccaggtc
     1981 ggccagcgcc cgcgccacgg aaagcaggaa ctcgttatcg ctgaactcga ggaatcctac
     2041 gttgtcttcc aacccgcatc agccggctcg gcggcggtta ccctagaacc agatacgcag
     2101 ccccagggcg gcctggatgt gccagtcgaa gcccgttggc accagggaaa gcccgaccgc
     2161 cggcgccacc tcgaggaaca cctcgaggga ctggggaatg ggccagatct ggagaccgaa
     2221 cgggaaacgg acgcccgccg tgaagggcgc tccgaacgta gcgtatccgc cgaacccacc
     2281 gtacccggag aggaaccggt tgaagctccc cttcagagcc cagtagtcgg cggtgaaccc
     2341 gatggccagg ggcgatcccg ccaccccgac gccgaacatg agaggaacct ggggcaggtg
     2401 gaacaccagc attccgctgg ccgggagccc gccggctccg tcgatgtaga acccaccctc
     2461 gcctccgagg gcccagcccg cagccgcgaa cgctccgcag gcgcacagaa ggatgagagc
     2521 agcgcagatc accgcttttc gcatgccggg aagtatacca gagacggggg agcgccacca
     2581 gaacggaagc cgacgccgca gggtgcaggc ggcacggccg gatcaggcgc cgaccggcgg
     2641 cctcgccgac ccgccgagat cgattgacac cgcccgtgct tgggggtaca agggaggata
     2701 ccatgaccaa cgcagaatcc tcgcgcgcgc tgctctcggg caacgaggcg atagctcgtg
     2761 gcgcctggga agccggtgtt accgtggcta ccggctaccc ggggacccct tccaccgaga
     2821 tcctggaggc ggtcaaggcc tcctaccagg acatctactg cgaatggtcc cccaacgaga
     2881 aggttgccct ggaggtggct gccggtgctt ctctggcagg agcccgcgtc atcgtgacca
     2941 tgaagcacgt ggggctgaac gttgcggcgg atcctcttct gacccttgcc tacatcggca
     3001 cggtgggcgg gctggtgatc tgcgttgccg acgatccggg catgcattcc tctcagaacg
     3061 agcaggacac gcgccactac gcgcggttcg ccaaggtccc catcctggag ccatcggact
     3121 cccaggaggc caaggacttc ctggcgttgg ggctggagat ctccgagcgg cacgggaccc
     3181 cggtgcttct tcgctctacc acgcgggtca gtcactccag cggctcggtc cagttggggg
     3241 agcgaaagcc ggtgccggcc tccgacggtt tcaagaagga tccgaagcgc ttcgttccca
     3301 tccccatgaa cgcccgcccc atgcgggacc ggctggaggt ccgcctgcag gcgctcgccg
     3361 gggaatcatc cgccagcggc gcaaaccgta tcgagtgggg cgaccggaag gttggtgtca
     3421 tcgccgaggg catcgcctac caatatgtcc gggagaccat gccggacgcc tccgttctga
     3481 agatcgggtg ggtatggccc ttccccgatc cgatcatccg ggagttcgcc gccggagtcg
     3541 aacggttgct ggtggtggag gagctggatc ccatcctgga ggagcacatc cggtccctcg
     3601 ggattgcgtg cgagggcaag agcaaggtgc cggccatcgg ggagctctcc ccggaccggc
     3661 tgggcgccgc cctggggttg accatgcctg cctctcggcc ggccgccgcg gacttgcccg
     3721 cgcgcccgcc ggtcctctgt ccggcgtgcc ctcaccgcgg gatcttctac gccctgggcc
     3781 gtctggacgt ggcagtgacc ggggacatcg gctgctattc cctgggcgtc ttcccgccgc
     3841 tgaagcgcat cgacacgatc ctctgcatgg gcgccgggat ttccatggcg catgggatgc
     3901 aggtagtcca cgcacggacc agcaccaagg agcaccccag acagaaagtc gtcggtatcg
     3961 tgggggattc gacgttcttc cattccggga tcacgggtct cctcgacatc agctacaacc
     4021 tgggcgcggc caccgttatc gtcgtggaca accgaaccac cgccatgacg ggtcaccagg
     4081 agcacccggg caccggcaag accatccgtg gggagcctac cgtcgaggca agcatcgagg
     4141 acttcggccg ggcctgcggg atgaagaaca tcgccacggt ggatccgtac gacctggacc
     4201 agactctcaa agtccttcgc accgcggtgg actcggatga gccgtggctc atcgtgtctc
     4261 gttctccctg tcccctgtac accaaggagc gagtcggccc ggcacgtcag gtggaccagg
     4321 agttgtgccg caagtgcaag gcttgcctga agctggggtg ccctgccatc gagtgggtga
     4381 acgacgagat ccacgtgaac gagcttctct gcggtggatg cgccctgtgc gagaaggtgt
     4441 gcaaggatgg cgcgttcgcc ctggcgaagg cagcgggagc gtgcgccgat gcgtgagacc
     4501 acgagcatgg tcctggtcgg ggtgggcggg cagggcatcc tgctggccac tcaggtagcc
     4561 gcccgcgcgg ccctgatcgc cgggatggac gtcaagacca acgaggtgca cggaatggcg
     4621 cagcgcggcg gctccgtggt cgcccaggtc cgctacggcc gcaaggtcta cagcccgctc
     4681 gtgccggatg ggagcgccga cgcgctcttg tccctggagc gcatcgaggc cctgcggttc
     4741 gccagtttcc tggcgccggg cggactggct gtggtctccc gtcaggccat cgtgcccgtg
     4801 accgtctcct cgggtcaggc cacctatccg tccgatgcgg aggagcgcct tcgggccgaa
     4861 ttcccccggc tggcgtatat cgacgcggtc gccgtggcgg agaaggtatg cgatatccgc
     4921 gttgccaaca tggtgctcct gggagcgctt tccacggcgc tctcccttcc gctggagtgc
     4981 tggtctcggg cgatcgccca gtgcgtgaag ccgaagttcg tggagatgaa cgaacgagcg
     5041 ttccaggagg gccgttcccg ggcgccaacc tcctgagggt cgctcctatc cccagcgccg
     5101 cagcgcctcg acgagccgat ggaactccgc ggccggaaac gggtcgtcgc ggatccggat
     5161 gcggttgccg gtagccagga cgattgtcac cgagcctctg cccagtctcc gcttgatgcg
     5221 aacggaaatc accgagcgac ggtccagcct ggattctctc agttctctac ctcggtacat
     5281 cgtgagagtt tctggttcca gctcgatgga gcggatgcct cccctcgtcc aaagcaccgc
     5341 atccaggacg agcagcgccg cggctcccag gatcaggagc gccacggcta gcggagaaag
     5401 gagcaggggc acccggtaca ggacgtagag cgcgagcacg cataccagga acaccgcgcc
     5461 gtccaggacc agcaggttgc ggagccggcc ttcgacccgc accgcgccat ccacccgcgg
     5521 agtttccaga ccgtccgtgc gcagcgttcg cgagcccccc atgcgagggt tatagcccaa
     5581 cctcgctcgg cttgacaggg gccgccggcg ccgatacttt ggttcgaccc cgctgggggc
     5641 atccagggag aggattcacg gcagaagtga ccaaagcggt tcttgcgctc gaagatggtg
     5701 cgttctttga gggggaggcg ctgggcgctc caggagaggc gttcggcgag gtcgttttca
     5761 acacgtcgat caccggatac caggaagttc ttaccgatcc atcctatcgc ggacagatcg
     5821 tcaccatgac ctacccgctc atcggcaact acggcatggt cccggaggac gatgagtcgg
     5881 atcgcccgtg gctctccggc ttcgtcgtcc gggaggccag cgccatccgt tccaactggc
     5941 gctcgaccgt gggtctggcg gactggttgt cccagctcgg cgtcgtgggg atccaccgca
     6001 tcgacactcg cgcgctcacc cggcatttga ggatcgaggg tgccaagcat ggggtcatat
     6061 ccaccgtcga cttcgaccga gcgtccctgg ccagcaagct gcaggcttcg ccccggatcg
     6121 tgggacggga cctggtgcgg gaggtgagct gcgaggagcc ctaccgctgg gccgagccgc
     6181 tgccagtacc gcggacacct gccgcggggg cgcccgtccc gcggacacac gcgcttccca
     6241 cggatccgcg gcgccacgtc gtggtgctgg actgcggcgt gaagcgcaac atcctccgtc
     6301 acctggtgtc cgcggggtgc gccgtcaccg tggtgccggc aaccactccg gccgctcaga
     6361 tcctggcgct caacccggat ggcctgctcc tgtccaacgg accgggggac cccgaaccgg
     6421 tcacctacgt cgtggaaacc gccaggagtc tcctgggccg gctgccgatc ttcgggatct
     6481 gcctgggcca gcagatgctg ggcctggcca tgggcggcaa gacctacaag ctgaagttcg
     6541 ggcatcacgg cggcaaccac cccgtcaaag atctcgccac cggaaggatc gcgatcacca
     6601 cgcagaacca cgggttcaac gtggacctgg agt
//
LOCUS       DASRZS010000144         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3160817, whole genome shotgun sequence.
ACCESSION   DASRZS010000144 DASRZS010000000
VERSION     DASRZS010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3160817"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VFH83_03630"
     CDS             complement(<1..1380)
                     /locus_tag="VFH83_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6485484.1"
                     /translation="MGKRRGILVLAMLVCGVLFLASGAYGAPQAPTEVRMTILADPGV
                     VDPATNWVYEVGHNMFVTLVGYDYVKNEVTPRGAVSWKVSADNLVWTFSIRKNWNWSD
                     GRPVTAKDYEYSFQEVVNPANASPTASFLFIVKNGEDVSQGKKPLSDLGVKALDDYTL
                     QITLTAPAAWFLSSLSSIGYAVPKWARDKSGKDWTRPENIVVNGPYKVTKWVNDSEVD
                     LTKNPSYYDAANVQVDKMTLYIVASEATAEAMYEKGDIDTVNVPATDLDRVQKDPVLS
                     KEFSSTPQLRNTMFRFYVAKPPFDNLNVRKAFAMAVDRQTLVSTITRGNEIPAYTMTP
                     PGCIGYVAPDAGIGIKFDPKGAAKLLADAGYPGGKGLPPIVYGYNASETNARIAQALQ
                     KMWKDTLGADVTLQGYEGGGYGDVVQSGAVNVYRWGWGMDYTDANNVWTNLFTSDVAF
                     KGTVVPDQLD"
     gene            1620..2606
                     /locus_tag="VFH83_03635"
     CDS             1620..2606
                     /locus_tag="VFH83_03635"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6485485.1"
                     /translation="MASISLTDYIINDLKRRIQSGEDVSSRLSLAELAKDYGVSITPL
                     RGAMNALIAGGFVEKLPNRRLRLGKRRGKSARGGAPSRPVPVPPTPSDWDSALLKEVT
                     LSSLRPHATYMREEVMARKLSAGRSIIRQAFNRFAGVGLIEHVPRRGWLVRPLSPQDM
                     NAYLAVREVLELKALELARGRLELSDLKELIASNGRPGVRAASQLDNRLHDYVIEKSG
                     NRYLRLFFQQYNARYYTELFYHAAPKTSVVGQMVQQHHGILQALVDGDWTRARGLLSE
                     HIRAQGPILMKLLARESVEYERKDAIKQGRGAGRPARGRGKPRRRDRPGEGA"
BASE COUNT          422 a          845 c          878 g          489 t
ORIGIN      
        1 gtccaactgg tcgggcacca cggtcccctt gaacgccaca tcggaggtga acaggttggt
       61 ccacacgttg ttcgcgtcgg tgtagtccat tccccagccc cagcggtaca cgttcacggc
      121 cccggattgg acgacgtcgc cgtagccgcc gccttcgtac ccctggagcg tgacatccgc
      181 gccgagcgtg tccttccaca tcttctgcag cgcctgggcg atccgggcgt tggtctcact
      241 ggcgttgtac ccgtagacga tcgggggcag gcccttcccg cccgggtatc ccgcgtccgc
      301 caggagcttc gcggcgccct tggggtcgaa cttgatgccg atcccggcat ccggagcgac
      361 gtaaccgatg caccccggtg gcgtcatggt gtaggcggga atctcgtttc ctctggtgat
      421 ggtggacacc agcgtctgac ggtcgacggc catggcgaag gctttgcgca cgttcaggtt
      481 gtcgaacggc ggcttggcga catagaagcg gaacatcgtg tttcgcagtt gaggggtgct
      541 ggagaactcc ttgctcagga cgggatcctt ctgcacgcgg tccaggtccg tcgcgggcac
      601 gttgaccgtg tcgatgtcgc ccttctcata catcgcctcg gccgtcgcct ccgatgccac
      661 gatgtacagg gtcatcttgt cgacctggac gtttgccgcg tcgtagtagc tcgggttctt
      721 cgtcaagtcc acctcgctgt cgttcaccca cttcgtgacc ttgtagggac cgttgacgac
      781 gatgttctcc ggccgggtcc agtccttccc ggacttgtcg cgcgcccact tgggcaccgc
      841 gtatccgatg gaggagaggg agctcaggaa ccacgccgcc ggggcggtga gggtgatctg
      901 cagcgtgtag tcatccaggg ccttcacgcc gagatccgaa aggggcttct tgccctggct
      961 cacgtcctcc ccgttcttga cgatgaacag gaagcttgcc gttggagagg cgttcgcggg
     1021 gttcacgacc tcctggaagc tgtactcata gtctttcgcg gtgaccggcc ggccatccga
     1081 ccagttccag ttctttcgga tggagaaggt ccagaccagg ttgtccgccg agaccttcca
     1141 cgagaccgcc ccgcgggggg tgacctcgtt cttcacgtag tcatagccca ccagggtgac
     1201 gaacatgttg tggccgacct cgtagaccca gttcgtcgcg ggatccacga cgccggggtc
     1261 ggcgaggatg gtcatcctca cctcggtggg ggcctgcggg gcgccgtacg cgccggaggc
     1321 caggaacaga acgccgcaca cgagcattgc cagaacgagt atccctcgtc tcttccccat
     1381 aggaacctcc tggatttcct ggattgcgtg cgcgcgggcc caggcccgcc gtgcccgcgt
     1441 cggatcgaga ccctgtgtcc gccctgcctc ctcctttgcg gtaggttttt atcttgttga
     1501 caagatatcg attccatcat accctgtccc cgggggttgt caagagggac ggagcgtctt
     1561 cttgggggga ggccgctgcg gcgccccacg gcgcacgcca gcttcgaaag gcacggtgca
     1621 tggcctcgat cagcctgacg gactacatca tcaacgacct caagcgcagg atccaatcgg
     1681 gggaggacgt ttcgtcgcgg ctctccctcg cggagctggc caaggactac ggggtcagca
     1741 tcacgccgct gcggggagcc atgaacgccc tgattgcggg cgggttcgtg gagaagcttc
     1801 ccaaccggcg gctgcggctg gggaagcgcc gcgggaaaag cgcgcgcggc ggggcgccgt
     1861 cccggccggt gcccgtcccc ccgactccga gtgactggga ttcggctctg ctgaaagagg
     1921 tgacgctttc gagcctgcgt ccccatgcca cgtacatgcg ggaggaggtg atggcccgca
     1981 agctctcggc cggcaggtcc atcatccgcc aggccttcaa ccggttcgcc ggggtggggc
     2041 tcatcgagca cgttccgcgg cgcgggtggc tggtgcgccc tctgtccccg caggacatga
     2101 acgcctacct tgcggtacgc gaggtcctgg agctcaaggc cctggagctg gcgcggggcc
     2161 gcctggagct gtcggacctg aaggagctga tcgccagcaa cggacgcccg ggtgtccgcg
     2221 ccgcctccca gctcgacaac cggctgcacg actacgtcat cgagaagtcg ggcaaccgct
     2281 acctgcggct cttcttccaa cagtacaacg cgcggtatta cacggagctc ttctaccacg
     2341 ctgccccgaa aacctccgtg gtgggccaga tggtccagca gcaccatggg atcctgcagg
     2401 cgctcgtcga cggagattgg acgcgggcgc ggggactgct ctcggagcac atacgcgccc
     2461 agggaccgat cctcatgaag ctgctggcgc gtgagagcgt cgaatacgag cggaaggacg
     2521 cgatcaagca ggggcggggc gcggggcgcc cggcccgtgg ccgggggaag cctcggcgaa
     2581 gggatcgacc aggggagggt gcatgaagcg tctggaggga gtgttcgtcg tgac
//
LOCUS       DASRZS010000145        12489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3161088, whole genome shotgun sequence.
ACCESSION   DASRZS010000145 DASRZS010000000
VERSION     DASRZS010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 12489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12489
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3161088"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VFH83_03640"
     CDS             <1..830
                     /locus_tag="VFH83_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HET6485486.1"
                     /translation="DIIQITPLWKGDRFDDGRPRVDDDIVRRMRFCTTEEAWGYPYER
                     GYKFQWEGDWFMPHPDRVLVGRAVTAVFVPQRPDLHDYLMEYGHAQERRIGAMNSWVI
                     QTVQKGDVVVVDLFHKIFEGTFTGGNLSTAISSRGGAGQVIYGGMRDLQQVETIKDFV
                     TFCKGIDPTGINNVTLVGLNVPCRIGRATCLPGDIVHGTKSGITFVPAHLAQATVEFS
                     EKTRMREAFGFAMVRKGKYDSSQVDTEWTREMEEEFLDWRKKLSPKEVERILWEVSY"
     gene            complement(906..2726)
                     /locus_tag="VFH83_03645"
     CDS             complement(906..2726)
                     /locus_tag="VFH83_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485487.1"
                     /translation="MARRQFFDDEEMQEVSGKTLWRLLRYLAPYKSKVIFSVGLLLLA
                     ALAAQIGPYLVKLAVDVYMPRKDIGGIALIAGLFAAVLLASAWAMRARFSIMVRLGNK
                     VIEDIRDQLFRHVNRLPFKFFDDRPAGKIIHRIMVYVDRLQQLVKHGVVSIIADVFRL
                     VIILGFMFAISVQLSLIALTVTPFMVAFVFFIKGAIHKRWDINQYKSANLNAYAHESF
                     IGIKVTQAFVREEKNSGIMREQLQENYTTWMRAVNLSNVLFPAVLLFNTVSIALCYWF
                     GFRFLGMGKASLGTLIAFSSYIWMITDPIVDLSNFYNDVLVALAAADRIFDYLDTPIT
                     TSDRPGAYTLPPLRGTVEFRDVHFAYDPKVPVLRGLTFSVEAGMTVALVGRTGAGKST
                     IINLISRFYEIQSGQILLDGHSIQDVTLESLRGQVGVMMQDPFVFTGTIADNIRYGRL
                     QASDAEVIEAARAVHAHEFVEEMPGGYEAQLKEAGNNLSVGQKQLLSFARTLLMDPRI
                     LILDEATASIDTRTELLVQQAIRRILQGRTSFVIAHRLSTIRNADIIFVIEGGRIVES
                     GKHDELLRLDGRYRELSATQALDPAATRGRGTRWAVSERS"
     gene            complement(2726..4489)
                     /locus_tag="VFH83_03650"
     CDS             complement(2726..4489)
                     /locus_tag="VFH83_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485488.1"
                     /translation="MSLPKALRFARPHRWWAAASVVSAILVVGTALVPPALSKRLVDG
                     VFVASVNDGNFASRRGLLLVLIGALVAVNALRACMIFLRNTSVEVYSQRVTRDLKQHL
                     YDHIQSQSFDFFHNTRTGELMARMTNDVEMIRSLLTQGLLNGVGGAFFIVGSAIVLSV
                     LNWQLTLVAVVAAPLLFWVAIRLRLDMREPTQAVRQQFSTLNTTVQENISGIRVVKAF
                     MRHAHEHGKFRRDNESLTTKRNTNMGVWARYMPHLEFLSNIASAMVLFAGGWLVIRGR
                     ITLGTWVQFNGYLWMLVQPMRGLGDVANQVAMASASAERIWDILELQPNIASPRTPRR
                     PARIAGDVELRGVCFSAGDRPILKNINLHAPPGSTIAIMGPTGSGKSSLVHLITRFYD
                     PTAGQVLIDGIDARELDLAFLRENVGFVAQETFLFSETLYNNLTYGKEGASLELVEKV
                     ASQTQVDEFIRPLADGYDTIVGERGVGLSGGQKQRASLARALMKQAPILILDDSTSSV
                     DMETEARIQAALRNLDHRATTFIIAHRISSIIHADQIIVLDHGEIVEQGHHEGLLERR
                     GLYAEYYDVQQTQRARGDGGR"
     gene            complement(4565..5239)
                     /locus_tag="VFH83_03655"
     CDS             complement(4565..5239)
                     /locus_tag="VFH83_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HET6485489.1"
                     /translation="MRDDLDLRGTLRFLGQLKQNNNREWFEKNREQYEEAMAQFELLV
                     GRLISDIGKSVDLDGITPRDCIMRIYRDVRFSKDKSPYKTGLGAGIVPGGRKSGRMGF
                     HLHVGPGGATMVGSGLWEPTPQQLTRFRNAISEDAGAFRRIVAAAPFKRHFGEVMGDR
                     LRTAPQGYPADHPAIELLRLKQVCVAEKFDDDTVVSRAFPGLAVESMKVMRPFIAYLE
                     EVARGA"
     gene            5523..7823
                     /locus_tag="VFH83_03660"
     CDS             5523..7823
                     /locus_tag="VFH83_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6485490.1"
                     /translation="MGIGTRLIVLISTVVAIVFAAAAYVSISRAGALAMGDARTIALT
                     TAQLEAAYVNQTVSEAAENASALARVFEAGANAGSVSRDTANAILKRFIERNPGFLAA
                     YVGFEPDAFDGADSRFRGRSGYDDTGRFLPHWSRDHNGSGVEEPLRGYDTLGAGDYYQ
                     LPRQRMMTCIIDPRPYQVLGKSVLMTSVVSPILAPGGGFLGIAGVDLDLSVMQSQLRL
                     NRIGDYADAYLQLFSSNRTVVADQDAASLGKPLAQGARDAGYAANVGRSESFTMVRES
                     GGPSGTVMSVGYPFKVADSGQVWMLVVNIPMSTLTAASSQLTLELAGIAAVAVLIVIA
                     VVFLISRSLIRPLSRGVAFAERVAKGDFRQKLAVPRRDEMGRLTSALNGMSVRLSDTV
                     ATLQESAQQVAASSERITSGARRLADGAQSQATTLEETSAAVEELTASVGQVAEHAHS
                     QAAAVEQGTASMAEVRRLMQEVSSNLNEIAGLAGKSVEQALQGAQAVSDVVDGINLIA
                     SSSGKIGGIIEVIDEIADQTSLLALNASIEAARAGEHGKGFAVVASEVSKLADRSATS
                     TKEIGTLIEESANNVKRGVTTARGSQLAMELIRAASQQEQDTIEKLAALMKQQVTAVE
                     ELTKALDGIAEMSQSISAATEEQTANAQQVSRAVENVNEVTQGTASAAEDMAASTEQL
                     SQMAQKLQRMADQFQIRGLRRANDKPERGGKNGKPPAEPRRDPPPRQKPSSKAERPPT
                     VEPSPELEATTLESRPSQEPSGWEES"
     gene            8192..9346
                     /locus_tag="VFH83_03665"
     CDS             8192..9346
                     /locus_tag="VFH83_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485491.1"
                     /translation="MGDGNRGRFQAIIDENPRAQAACRRVARVIRGEVPDRVPFIDHY
                     WAEFAERYRRERGLPADTSLEERFDHDLWILDPVMGPWPSEARVIRTEADGYVISRDE
                     FGLLLRSHETRQVVPNQLDYRIKERRDLDRFPFEDPADPSRSTAVEAALERTCRRFCP
                     VFKLGGPFSRTWRLRGLSRFLEDIAEDEGFAKEMTARMTDHLIAVGAATVERLDWPRY
                     QMHIADDFACTASPLFSPAAYERIVLPNLKRMVDRFHALGFRVSYESEGNVFPMLELL
                     DQSGVDGLAHMEPRAGMTLKRIRERFGGRFFVMGNICNTMVLPSNDRRRIAAEVLRVL
                     SAARDGHYLGLSAHSVGTDISSDSYDYFYGLMTRFGRYPMDLAELEREAR"
     gene            9394..9855
                     /locus_tag="VFH83_03670"
     CDS             9394..9855
                     /locus_tag="VFH83_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET6485492.1"
                     /translation="MKITLTMDQSEHFRDYLRRNGLAFTTSRRTILTWITEATGHFDT
                     EELRESLARHGRKLSPATIYRTLPLFVRSGIIKETIRTGDKARYEHVYGHEHHDHLEC
                     LRCGRIIEFMDPGLERLQEKVCRKHRFTPVEHRLAIRGYCSRCMPRKGRRG"
     gene            9852..11789
                     /locus_tag="VFH83_03675"
     CDS             9852..11789
                     /locus_tag="VFH83_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HET6485493.1"
                     /translation="MTRPEATAQKVRRVVIVGLPNSGKSTLFNALTGEYDLVANYPMT
                     TVEVKRKECGIEGRPFEIVDTPGLHGLFIQSEEEMVVRSLLFQETSDLFVQCMDANRL
                     KESLVLTADLLDLGVPLVLYVTAVREGANRGTAIDAPALSRALGVPVVESTGPGKAVA
                     EVTRAILSPPPAARAVEIGSRLEQAVHRVEATLPAGSPFPRASALLLLEGDPSFPIPS
                     NGNGAEARTELSGIQAGFAGKAGLLMSSQRSHWVDRVAESVVSHSPRPKSSFSESFAS
                     LCRHPIYGLPMLALFLVLVYLLVVQGAGTLSLLLTTAVTDPVVRAVSHILPVGFWRDL
                     LVGPRGLLTLGLFNSLTTVLPVLSLFFLLFGLMEDIGYLPNLTILTRRAFGKIGLSGG
                     AIIPIVLGFGCKTMATMTARSIPSRREKLIAVALIAFAIPCSAQMGLSMAILGQHGFR
                     YFFLAFLVLGLAEVAVGFLLNKLLPKGPKDRFIQELPPIRLPSLVAVGKKTGYRLLWF
                     LKEAIPIFILAAFVLFVIDRIGILGLLKDALRPLIVGRLGLPLDMVDALLLTLARSEA
                     GAGLILRMSHSGVLNGVQSIVAVVLLTTFSQCFANIGAMLKELGARVGLTIVASIYVA
                     AFLVAGATHALLGLLSGVLKL"
     gene            11786..>12489
                     /locus_tag="VFH83_03680"
     CDS             11786..>12489
                     /locus_tag="VFH83_03680"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET6485494.1"
                     /translation="MNGSNLRDELLECLWQLRERDTSTVHGLQEALGERYRPSDLDSA
                     VADGLARREQERISLTDSGSAAAEKLIRAHRIGERLIHDVLGREFESGACEFEHLVNT
                     EIVDGICTLLGHPRECPHGFAIPEGDCCRASARYAQRTIVPLTELKIGQSARVAYVYT
                     NNDQELHRLASLQIKPGAVIKLHQQYPSYVIDCEGTSIALDGEVAGNVNVWLEGAQSP
                     DASEAAAERRHWAKRR"
BASE COUNT         2140 a         4126 c         4120 g         2103 t
ORIGIN      
        1 aggacatcat ccagatcacg ccgctgtgga agggcgaccg gttcgacgat gggcggccgc
       61 gcgtggatga cgacatcgtc cgccggatgc ggttctgcac gacggaagag gcctggggct
      121 acccctacga gcgcggctac aagttccagt gggaggggga ctggttcatg cctcaccccg
      181 accgcgtcct ggtgggacgc gcggtgactg ccgtgttcgt cccccagcgt cccgacctgc
      241 acgactatct catggagtac ggccacgcgc aggagcgccg catcggagcc atgaactcct
      301 gggtgatcca gacggtccag aagggcgatg tcgtggtggt ggacctgttc cataagatct
      361 tcgaggggac gtttaccggt gggaacctct ccaccgccat ctcctcccgc ggcggcgccg
      421 gccaggtcat ctatggcgga atgcgggacc tgcagcaggt ggagaccatc aaggacttcg
      481 tcaccttctg caagggcatc gatcccaccg ggatcaacaa cgtgaccctg gtgggtctga
      541 acgtcccttg ccggatcggg cgggccacct gtctgccggg ggacatcgtg cacggcacca
      601 agagcggcat caccttcgtc cccgcgcacc tggcgcaggc aaccgtcgag ttctcggaga
      661 agacgcggat gcgcgaagcg ttcggcttcg ccatggtgcg caaggggaag tacgactcca
      721 gccaggtgga caccgagtgg acccgggaga tggaagagga gttcctcgac tggaggaaga
      781 agctcagccc caaagaggtc gagaggatcc tctgggaggt ctcatactag gccaccgggg
      841 cggggcactg ggtcgggggg cactgggtcg ggggccgctg ggcgcaggcc tccgacgacg
      901 ggttgctacg agcgctctga aacggcccag cgtgttccac ggccgcgagt ggcggccggg
      961 tccagggcct gggtcgcgct gagctcgcgg taccttccgt cgagcctcag aagctcgtcg
     1021 tgctttccgc tctcgacgat ccggcctccc tcgatcacga agatgatgtc cgcgttgcgg
     1081 atcgtggaga gccggtgggc gatcacgaac gaggttcggc cctggaggat ccgccggatg
     1141 gcctgctgca ccagcagctc cgtgcgggtg tcgatgctgg ccgtggcctc gtccaggatg
     1201 aggattcgcg ggtccatgag gagcgtccgg gcgaaggaca ggagctgctt ctgccccacg
     1261 gacaggttgt tcccggcttc cttgagctgc gcctcatagc cccccggcat ctcctcgacg
     1321 aactcgtgcg cgtgtaccgc gcgggccgcc tcgatcacct ccgcgtcgct ggcctggagc
     1381 cttccgtagc ggatgttgtc cgcgatggtg cccgtgaaga cgaaggggtc ctgcatcatg
     1441 acgcccacct ggccccgcag ggattccagg gtgacgtcct ggatgctgtg gccgtccagg
     1501 aggatctgcc cggactggat ctcgtagaac cgggagatga ggttgatgat ggtgctcttc
     1561 ccggcgcccg tcctccccac cagggccacc gtcattccgg cctccacgga gaaggtcaga
     1621 ccccgaagca ccgggacctt gggatcgtac gcgaagtgca catcccggaa ctccaccgtg
     1681 cccctcaggg gcgggagcgt gtacgccccg ggcctgtcgc tggtggtgat gggggtatcg
     1741 aggtagtcga agatccggtc cgcggccgcc agggccacca ggacgtcgtt gtagaagttg
     1801 ctgaggtcca cgatggggtc ggtgatcatc cagatgtagc tggagaacgc gatgagggtc
     1861 cccaggctgg ccttccccat cccgaggaac cggaacccga accagtaaca cagcgcaatg
     1921 gacaccgtat tgaagaggag cacggccggg aagagcacgt tggacaggtt caccgcgcgc
     1981 atccaggtgg tgtagttctc ctggagctgc tcccgcatga tccccgagtt cttctcctcc
     2041 cggacaaagg cctgggtgac cttgatcccg atgaagctct cgtgggcata cgcgttcagg
     2101 ttggcgctct tgtactggtt gatgtcccac ctcttgtgga tggcgccctt gatgaagaac
     2161 acaaacgcca ccatgaacgg ggtcacggtg agcgcgatga gggagagctg cacggagatg
     2221 gcgaacatga agcccaggat gatgacgaga cgaaagacgt cggcgatgat gctcaccacg
     2281 ccgtgcttga ccagctgctg cagccgatcc acgtacacca tgatccggtg gatgatcttg
     2341 cccgcggggc ggtcgtcgaa gaacttgaac ggcagacggt tgacgtggcg gaagagctgg
     2401 tcgcggatgt cctcgatgac cttgttgccg aggcggacca tgatcgagaa gcgcgcccgc
     2461 atggcccagg cgctcgccag gagcaccgcg gcgaacaggc ccgcgatcag ggcgatgccg
     2521 ccgatgtcct tccgcggcat gtacacgtcc accgccagct tcaccaggta cggaccgatc
     2581 tgcgcggcca gcgccgccag caggagcaag cccacggaga agatgacctt gctcttgtag
     2641 ggcgccaggt agcgcaggag ccgccacagc gttttgccgc tgacctcctg catctcctcg
     2701 tcgtcaaaga actgtcgtct ggccatcagc ggcctccgtc ccctcgcgcg cgctgggtct
     2761 gctggacgtc gtagtactcc gcgtacaggc cccgccgctc caggagtccc tcatggtggc
     2821 cctgctccac gatctccccg tggtccagga cgatgatctg gtccgcgtgg atgatggagc
     2881 tgatccggtg ggcgatgatg aacgtggtgg cccgatggtc gaggttgcgc agggcggcct
     2941 ggatcctggc ctccgtttcc atgtccaccg agctggtcga gtcgtcgagg atcaggatgg
     3001 gcgcctgctt catgagcgcc cgcgccaggc tggcgcgctg cttctgcccg ccggaaagac
     3061 cgacgccgcg ctcgcctacg atggtgtcgt acccgtccgc caggggccgg atgaactcgt
     3121 ccacctgggt ctggctggct accttttcca ccagctccag cgacgcgccc tccttcccgt
     3181 aggtgaggtt gttgtaaagc gtctccgaga acaggaacgt ctcctgggcc acgaacccga
     3241 cgttctcccg gaggaaggcc aggtccagct cccgggcgtc gatcccgtcg atcagcacct
     3301 ggcccgccgt gggatcgtag aaccgggtga tgaggtggac gagggagctc ttgccgctgc
     3361 ccgtgggtcc catgatggcg atggtggagc ccggcggcgc atggaggttg atgttcttca
     3421 ggatgggtcg gtcccccgcg ctgaagcaga cgcctcgcag ctccacgtct cccgcgatcc
     3481 gcgccggccg ccgcggcgtg cgcgggctgg cgatattggg ctgcagctcc aggatgtccc
     3541 agatcctctc cgccgaggca gacgccatgg ccacctggtt ggccacatcc ccgagaccac
     3601 gcatgggctg caccagcatc cagaggtatc cgttgaactg gacccaggtc ccaagcgtga
     3661 tccggccccg aatgaccagc caaccgccag cgaagaggac catggcggac gcgatgttgg
     3721 agaggaactc caggtggggc atgtaccgcg cccagacccc catattggtg ttgcgcttgg
     3781 tggtgaggga ctcgttgtcc cgccggaact tcccatgctc gtgcgcgtgg cgcatgaatg
     3841 ccttcaccac ccggatgccc gagatgttct cctggaccgt ggtgttgagg gtggagaact
     3901 gctggcgcac cgcctgggta ggctcccgca tgtccagccg caatcggatg gccacccaga
     3961 acaggagcgg cgcggccacc acggccacca gcgtgagctg ccagttcagc accgacaaca
     4021 cgatcgccga accgacgatg aaaaacgcgc ctcccactcc gttcaggagc ccctgggtga
     4081 gcaggcttcg gatcatctcg acgtcgttgg tcatccgcgc catgagctcg cccgtgcgcg
     4141 tgttatggaa gaaatcgaaa gactggctct ggatgtgatc gtagaggtgc tgtttgaggt
     4201 cccgcgtgac gcgctggctg tagacctcca ccgaggtatt gcggaggaaa atcatgcagg
     4261 cgcgcagggc gttcacggcg accagggccc cgatgaggac gaggagcagc ccccgccggc
     4321 tcgcgaagtt gccgtcgttg accgaggcca caaagacacc gtcgaccagg cgcttggaca
     4381 acgcgggagg gacaagggca gtgcctacca cgagaatcgc ggataccaca gaggcggccg
     4441 cccaccaccg gtgggggcga gcaaatcgca gagccttggg gagagacata tctctacgga
     4501 agctcctgca ggaagacgac gcggtacact gagcacaaaa ggtagcgcgg agcgggggtc
     4561 ttgttcaagc gccgcgggca acctcttcga ggtacgcgat gaacggtctc atcaccttca
     4621 tgctctccac cgcaaggcct gggaaagccc tcgatacgac cgtgtcatcg tcgaacttct
     4681 ccgccacgca cacctgcttc aagcgaagca gctcaatggc gggatggtcg gccgggtacc
     4741 cctgcggagc ggtcctgagc cggtctccca tgacttcccc gaagtggcgt ttgaacggag
     4801 ccgcggccac gatccttcgg aacgcgcccg cgtcctcgct gatggcgtta cggaaccgcg
     4861 tgagctgctg aggagtcggc tcccacagtc cgctgcctac catcgtggcc ccaccaggac
     4921 caacgtggag gtggaagccc atgcgcccgg actttcgtcc gcccgggacg atcccggcgc
     4981 ccagcccggt cttgtagggg cttttgtcct tggagaacct cacgtcccgg tagatccgca
     5041 tgatgcagtc ccggggcgtg ataccgtcca ggtcgacgct cttgccgatg tccgagatca
     5101 gccggcctac cagaagctcg aactgcgcca tcgcctcctc gtactgctcc cggttctttt
     5161 cgaaccattc ccggttgttg ttctgcttta gctgacccag gaacctcaga gtgccgcgaa
     5221 gatccagatc gtctctcatc gaacccctcc aggccgctgc ccctgtctct acccgtgaac
     5281 ctaaccagca accagccacc ggtcaaactg ttccatgcgt acggaacact tcgttccata
     5341 ctgggcgcac cggatgctca tccgcttcga caatggtggt cgaacacgct cccaggcgcc
     5401 aaacgggcgc gcaggaaccg ttggggcgcg cgaatccagg tcctttgctg gcattcaggg
     5461 tcacccggtg tctcatcctt ggcatgcgaa ttgctcattc gagtgggggt aacccgaatc
     5521 ccatgggtat cggaacaagg ctgatcgtgc tcatttccac cgtcgttgcg atcgtcttcg
     5581 cggccgcggc ctatgtctcc atctctcggg ccggcgcgct cgcgatgggt gacgcgcgga
     5641 ccatcgcgct gaccaccgct cagctcgagg cggcgtacgt gaaccagacc gtgagcgagg
     5701 ccgcggagaa cgcctccgcg ctggctcgcg tgttcgaggc gggggccaac gccggttccg
     5761 tcagtcggga cacggcaaac gccattctca agcggttcat tgagaggaac ccgggcttcc
     5821 tcgcggccta cgtcgggttc gagccggacg ccttcgacgg cgcggactcc cgcttccgag
     5881 gcaggtccgg gtacgacgac accggccgct tcctgccgca ctggagtcga gaccacaacg
     5941 ggagcggagt cgaggagccg ctgcgcggct acgacacgct gggcgccgga gactactacc
     6001 agctcccccg ccagcggatg atgacctgca tcattgaccc gcggccctac caggtgctcg
     6061 gcaaatccgt cctcatgacc tccgtggtgt ctcctatcct cgcgccggga ggagggttcc
     6121 tggggatcgc cggggtggac ctggacctca gcgtcatgca gtcacagctt cgcctcaacc
     6181 ggatcggcga ctacgcggac gcctacctcc agcttttctc gagcaaccgg accgtcgtcg
     6241 ccgaccagga cgccgccagc ctgggcaagc cgctggcgca gggcgcgcgg gacgcgggct
     6301 acgccgccaa cgttggccgc agcgagtcct tcacgatggt gcgggagtcc ggcggaccgt
     6361 cgggcacggt gatgtccgtc gggtacccgt tcaaggtcgc cgacagcggg caggtatgga
     6421 tgctcgtcgt caacatcccg atgagcacgc tcaccgctgc cagcagccag ctcaccctgg
     6481 agctcgcggg gatcgccgct gtagcggtgc tgatcgtcat cgccgtggtc ttcctcatct
     6541 cccgctccct gatccgtccc ctctcccggg gggtcgcgtt cgccgagcgc gtggccaagg
     6601 gggacttccg ccagaaactg gcggtgccga ggcgcgacga gatgggccgg ctcacctcgg
     6661 cgctcaacgg catgtccgtg cgcctgagcg acaccgttgc cacgctccag gagagcgccc
     6721 agcaggtcgc tgcctccagc gagcgcatca cctccggcgc ccgacgcctg gcggacggcg
     6781 ctcagagtca ggccaccacc ctggaagaga caagcgccgc ggtggaggag ctgacggcgt
     6841 ccgtcgggca ggtggcggag cacgcccaca gccaggccgc ggcggtggag caggggacgg
     6901 cgtccatggc ggaggtgcgg aggctcatgc aggaggtctc cagcaacctg aacgagatcg
     6961 ccggcctggc gggcaagtcc gtggagcagg ccctccaggg cgcccaggcg gtgtccgacg
     7021 tggtggacgg gatcaacctc atcgccagca gctccggcaa gatcggcggg atcatcgagg
     7081 tgatcgacga gatcgcggac cagacgagcc tcctggcgct caacgcctcg atcgaggcag
     7141 cccgcgccgg ggagcatggg aagggatttg ccgtggtggc atccgaggtc agcaagctcg
     7201 cggaccgaag cgctacttcc acaaaggaga tcgggacctt gatcgaggag agcgccaaca
     7261 acgtgaaacg gggagtgacg acggcccgcg ggtcccagct cgccatggag ctgatccggg
     7321 ccgcttcgca gcaggagcag gacaccatcg agaagcttgc cgcgctcatg aagcagcagg
     7381 tcaccgccgt ggaagagctg accaaggccc tggatgggat cgccgagatg agccagagca
     7441 tatccgccgc aacggaggag cagacggcga acgcgcagca ggtgtcccgc gccgtagaga
     7501 acgtgaacga ggtcacccag ggaaccgcct ccgcggcgga ggacatggcg gcatcgaccg
     7561 agcagctctc gcagatggcc cagaagctcc agcggatggc ggaccagttc cagattcgcg
     7621 gcctgcgccg ggccaacgac aaacctgaac gcggggggaa aaacggcaag ccgccggcag
     7681 aaccgaggcg ggacccgccg ccgaggcaga agccgtcgtc gaaggctgaa cggccgccaa
     7741 cggtggagcc gtccccggag ctggaggcca cgaccctgga atcccgtcct tcccaggagc
     7801 cctcaggatg ggaggaatcg tgacggggcg ttgaccgcgc gcccgccgac cttgacaccc
     7861 tcaatggcgg acgggataat cgcgttttgc ggttccggcg ggcacggact gggactgggt
     7921 ggaccaggat ccgtgtttct caacggcccc tggagcccca acccggccca gcccaactgg
     7981 ctggaccagc agtacaacaa cggcaacgcc gtgatcgggc cgatgccgcc gctgtgggag
     8041 ccggactggt tcgtccccta cactttcaag ggaaccgcca ttgagaacgc ttttgcggac
     8101 gccaacaccc tgctctccga agcggaagtg aatatcatcc tgggcaagcg ggagtcggtg
     8161 tcgcgccgac tccccccttt caggagaccc tatgggcgat ggaaaccgcg gccggtttca
     8221 ggccatcatc gacgagaacc ccagggccca ggcggcctgt cggcgggttg cccgggtgat
     8281 ccggggagag gtcccggacc gggtcccctt tatcgaccat tactgggcgg agttcgccga
     8341 gcgataccgt cgggagcgcg gtctgcccgc ggacacttcc ctcgaggagc gcttcgacca
     8401 cgacctctgg atcctggatc ccgttatggg gccctggccg tcggaggccc gtgtgatccg
     8461 caccgaggcg gacggctacg tgatctcccg ggacgagttt ggcctgctcc tccggtccca
     8521 tgagacccgc caggtcgttc ccaaccagct cgactatcgg atcaaggagc gtcgggatct
     8581 ggaccggttc cccttcgagg atcccgccga cccgtcccga agcaccgcgg tggaagcggc
     8641 gctcgagcgc acgtgccgtc ggttctgccc cgtcttcaag ctgggaggtc ccttcagtcg
     8701 cacgtggagg ctgcgcggtc tgagccggtt cctggaggac atcgccgagg acgaggggtt
     8761 cgcgaaggag atgacggccc ggatgaccga ccatctcatc gccgtgggtg cagccacggt
     8821 ggagcggctg gactggcccc gctaccagat gcatatcgcg gacgatttcg cgtgcaccgc
     8881 atcgccgctg ttctcccccg ccgcctacga gcggatcgtt ctccccaacc tgaagcgtat
     8941 ggtggaccgg ttccatgccc tggggttccg cgtcagctac gagagcgagg ggaacgtttt
     9001 ccccatgctc gagctgctgg accagtccgg cgtggacggc ttagcccaca tggagccccg
     9061 ggcggggatg accctgaagc gcatccggga gcgctttggc ggacgcttct tcgtgatggg
     9121 caacatctgc aacacgatgg tgctcccgtc caacgaccgt cgacggatcg ccgcggaagt
     9181 gctgcgggtc ctgagcgcgg cgcgcgacgg ccactacctc gggctctcgg cgcactcggt
     9241 gggcacggac atctccagcg actcctacga ctacttctac ggcctgatga cccggttcgg
     9301 ccgctacccc atggacctgg cggagctgga gcgggaagcg cgctgagcac gccgcccggc
     9361 ttgatctttg cggccttgtg cgatagaatg agaatgaaaa tcactctcac catggaccag
     9421 tcggagcact tccgggacta cctgcgccgc aacggtctcg ccttcacgac gagccgacgg
     9481 accatcctca cgtggatcac cgaggccacg gggcacttcg acacggagga gctgcgcgag
     9541 tccctggcgc gccacggtcg aaagctttcc cccgccacca tctaccgcac gcttcccctc
     9601 ttcgttcgca gcgggatcat caaggaaacc atccggaccg gagacaaggc gcgctacgag
     9661 cacgtctacg gccacgagca ccacgaccac ctggagtgcc tgcggtgcgg gcggatcatc
     9721 gagttcatgg acccggggct ggagaggctc caggagaagg tctgccgcaa gcaccgcttc
     9781 acgccggtcg agcaccgcct ggccatccgc ggatactgct cgcgctgcat gccccgcaag
     9841 gggaggcgcg gatgacgcgc ccggaggcaa ccgctcagaa ggtcaggcgg gtcgtgatcg
     9901 tcgggctgcc caacagcgga aagagcacgc tgttcaacgc gctcaccggg gagtacgacc
     9961 tcgtggcgaa ctaccccatg accaccgtgg aggtgaagcg caaggagtgc ggcatcgagg
    10021 ggaggccctt cgagatcgtc gacacgccgg ggcttcacgg cctcttcatc caatccgagg
    10081 aggagatggt ggtccgatcc ctcctgttcc aggagacgtc ggacctgttc gtccagtgca
    10141 tggacgccaa ccggctcaag gagtccctgg tcctcaccgc cgacctcctg gacctcgggg
    10201 tcccgctggt cctctacgtc acggcggtcc gggaaggggc gaaccggggc acggccatcg
    10261 acgcccccgc gctctcacgg gcgctcgggg taccggtcgt ggagtccacg ggacccggca
    10321 aggccgtggc ggaggtcacg cgcgccatcc tctcaccgcc gccggcggcc cgggccgtgg
    10381 agatcggaag ccggctggag caggcggtgc atcgggtgga ggccactctt ccggcgggaa
    10441 gccctttccc cagggcttcg gccctcctcc tcctggaagg ggacccatcg ttcccgattc
    10501 cgtccaacgg gaatggcgcc gaggccagga ccgagctgag cggcatccag gccgggttcg
    10561 ccggcaaggc cggcctcctg atgagctcgc agcgaagcca ctgggtggac cgcgtggcgg
    10621 agtccgtggt cagccacagc cctcggccga agagcagctt ctccgagtcg ttcgcgtcgc
    10681 tgtgccgcca tccgatctac ggtctgccga tgctcgcgct cttcctggtc ctggtctatc
    10741 tgctggtggt tcagggagcc ggcaccctct cgctcctcct taccaccgcc gttacggacc
    10801 cggtggtgcg ggcggtgtct cacatcctgc ccgtcggctt ctggcgtgac ctcctggtcg
    10861 gaccgcgggg gcttctgacg ctgggtctct tcaactccct caccacggtc ctgcccgtgc
    10921 tctctctctt cttcctgctc ttcgggttga tggaggacat cggctacctc cccaacctca
    10981 cgatcctcac ccgccgggcc ttcgggaaga tcggcctcag cggcggcgcc atcatcccga
    11041 tcgtcctggg gttcgggtgc aagaccatgg caaccatgac ggcgcgcagc atcccgtcgc
    11101 gacgggagaa gctcatcgcc gtggccctga tcgcctttgc catcccctgt tccgcccaga
    11161 tggggctctc catggccatc ctgggccagc atggttttcg gtacttcttt cttgcgttcc
    11221 tggttttggg actggccgag gtcgcggtcg gattcctgct caacaagctc ctgcccaaag
    11281 gacccaagga ccggttcatc caggagctgc cgcccatccg gcttcccagc ctggtggccg
    11341 tcggcaagaa gacggggtac cgcctcctct ggttcctgaa ggaggccatc cccatcttca
    11401 tcctcgcggc gttcgtgctc ttcgtgatcg atcgcatcgg gatcctgggt ctgctcaagg
    11461 acgccctgag acccctgatc gtgggccggc tcggccttcc cctggatatg gtggacgccc
    11521 tgctgctgac ccttgccagg agcgaggccg gcgcggggct gatccttcgg atgtctcaca
    11581 gcggcgtcct gaacggagtg cagagcatcg tggccgtagt cctcctgacc accttctccc
    11641 agtgcttcgc gaacatcggg gccatgctga aggagctcgg ggcacgcgtc ggcctgacca
    11701 tcgtcgcgtc tatttacgtg gcggccttcc tggtcgctgg agccacccat gccctgctgg
    11761 gactcctctc tggagtgctg aagctatgaa cggttccaac ctgcgcgatg agctcctgga
    11821 gtgcctctgg cagcttcggg agcgggacac cagcacggtc cacggactcc aggaggcgtt
    11881 gggagagcgc tatcggccat cggacctgga ttcggccgtt gccgacggcc tggcgcgccg
    11941 ggagcaggag cggatctcgc ttaccgattc ggggtccgcc gccgcggaga agctcatccg
    12001 cgcccaccgg atcggcgagc ggctgatcca tgacgtgctg gggagagagt tcgagtccgg
    12061 cgcctgcgag ttcgagcacc tggtgaacac cgagatcgtg gatgggatct gtaccctcct
    12121 ggggcatccg cgggaatgcc cgcacgggtt cgcgatcccg gagggcgact gctgccgcgc
    12181 ttccgcccga tacgcccaga ggaccatcgt gcccctgacg gagctcaaga tcggccagag
    12241 cgcccgagtg gcttacgtct atacgaacaa cgaccaggag ctccaccgcc tggcttcctt
    12301 gcagatcaag cccggcgcgg tgatcaagct ccaccagcag tacccctcgt acgtcatcga
    12361 ttgcgagggc acgagcatcg ccctggacgg cgaggttgcc ggaaacgtta acgtctggct
    12421 ggaaggcgca cagtcgcccg atgcctcgga ggctgccgcg gagcgtcggc actgggcaaa
    12481 gcgtcgctt
//
LOCUS       DASRZS010000146         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_467942, whole genome shotgun sequence.
ACCESSION   DASRZS010000146 DASRZS010000000
VERSION     DASRZS010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467942"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..867)
                     /locus_tag="VFH83_03685"
     CDS             complement(271..867)
                     /locus_tag="VFH83_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485495.1"
                     /translation="MQHATPTFLYAEYEGAVSLVESYLVDPYDEVRFLSIGGPAEAVK
                     AVLAAAKLKGNQLILFHPKAHANLDRCGREYVGPLHATTTPSRLLCKRLPCGQTHGVL
                     YPDTIPSETPDAFSLVLPKTEEATAPERLLRLIDARTTLPLHPSWAGWLWRFLDENAD
                     ITPLSGAGHWVGWEIHWNEQQVTQELTEAVKARTLEIA"
     gene            complement(949..1998)
                     /locus_tag="VFH83_03690"
     CDS             complement(949..1998)
                     /locus_tag="VFH83_03690"
                     /inference="COORDINATES: protein motif:HMM:NF039653.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6094 domain-containing protein"
                     /protein_id="HET6485496.1"
                     /translation="MARFRSQAKMQYYPTPPPIAEAIARSLTRSGPGVIRAIDPACGE
                     GTALCLATGSLGDPVERYGIELNQERAQAARGVLTRVLRADIRSTRVANNAFGLLFLN
                     PPYDFDVPALDEGAQRLELSFLQAAVRYLAVGGVLVYLIPDRRLPSKIANVLTYHFER
                     IQVFRFPGETYNLYQQIVVFAVRKATPFRDDEALRTLRLIGARTITPPDLPGQVEPAY
                     SVPTSRRVANLLFQNLSVDPQDLVQELERHGLTQVVFRRLHPEGAAYRMRPIMPLRRG
                     HLALVLASGHLNNALVEDPRTGDRLLVKGRTEKDTLRLEEQEDDGGKTITERDILKIV
                     ITALDLRSGELQTMQ"
BASE COUNT          375 a          738 c          854 g          464 t
ORIGIN      
        1 gtcccgcacg ggatcgtgca ggatgttgaa ggtctcggtc acccgctggg ccagcggctc
       61 gccgaagcgc gtgaggaact cctggagagt catgtctggg tctccgtggg ggggaggggg
      121 aggtctgaag gctcggtacg cctcagcgtg tcgcgccgga gacgatcgag tgcgccgcga
      181 ccagtgcgtt cgctccggcc agtcttcagc gatcgtgcga gcgcttggtt cttgaggtgc
      241 cgcaagcttg gcatgccccc gccatggcgt ctacgcgatc tccagcgtgc gggcctttac
      301 ggcctccgtc agctcctggg tcacctgctg ctcgttccag tggatctccc atccgaccca
      361 gtggccagcg ccactcaggg gtgtgatgtc tgcgttctca tcgaggaagc gccagagcca
      421 gcccgcccac gacgggtgga gcggaagggt ggttcgcgca tcgatgagcc gcagcaggcg
      481 ctcgggtgcg gtggcttcct cggtcttcgg gaggacaagg ctgaacgcat cgggggtctc
      541 gctcggtatc gtgtcaggat agaggacgcc gtgagtttgc ccgcagggaa ggcgcttgca
      601 gaggagccgg gagggcgtcg tggtcgcgtg cagcggtccg acgtattcgc gcccgcaccg
      661 gtcaaggttc gcgtgcgctt ttgggtggaa gaggatcaac tggttccctt tgagcttcgc
      721 cgctgccagc acggccttga cggcttccgc cggccctccg atggacagga accgcacctc
      781 atcgtagggg tctaccaggt agctctccac caggctcacg gcgccctcgt attctgcgta
      841 caggaacgtc ggtgtcgcgt gctgcacggg ctcctcccaa gacggggtgc ggtctgcttg
      901 cgggcaagga gggatcgcat tgccgggggg ctagacggct ctgccgcgtt actgcatcgt
      961 ctggagttca ccagaccgga gatccagggc ggtgatgacg atcttgagga tatcgcgctc
     1021 ggtgatcgtc ttgccgccat cgtcctcctg ttcctccagc cggagggtat ccttctcagt
     1081 tcggcccttc acgagcagtc ggtcgccggt ccgaggatct tccaccagcg cgttgttgag
     1141 atgtccggat gccaggacca gcgccaggtg gcctcgccga agcggcatga ttggccgcat
     1201 ccgatacgcg gccccctcgg ggtgcaggcg gcggaacacc acctgggtga ggccgtgccg
     1261 ctccaactcc tggacgaggt cctgtggatc gacgctgagg ttctgaaaca ggaggttcgc
     1321 cacccggcgg gaggtcggta ccgagtaggc tggctccacc tgcccgggga gatccggtgg
     1381 cgtgatcgtc cgggcaccga tcaatcgcaa ggtccgcagc gcctcgtcat ctcggaacgg
     1441 tgtggccttg cgcacggcga acaccacgat ctgctggtag aggttgtagg tttccccggg
     1501 gaatcggaag acctggatgc gctcgaagtg gtacgtgagc acgttcgcga tcttgctggg
     1561 gaggcgccga tcgggaatga ggtagaccag gacgcctccc acggcgaggt agcgcaccgc
     1621 ggcctggagg aaagagagct cgagccgctg cgcgccctcg tcaagcgccg gcacgtcgaa
     1681 gtcgtagggc gggttgagga acaggaggcc gaaggcgttg ttggccaccc gcgtgctccg
     1741 gatgtccgcc cggaggaccc gggtgaggac tccgcgagcc gcctgcgctc gctcctggtt
     1801 gagctcgatg ccgtaccgtt ccacgggatc tcccagggag cctgtcgcga gacacaacgc
     1861 ggtgccttcc ccgcaggccg ggtcgattgc ccggatcacc cccgggcccg accgggtcaa
     1921 ggatcgggcg atagcctccg caattggcgg tggcgttggg tagtactgca tcttcgcttg
     1981 cgaccggaat cttgccactg gcctgactcc ttcgtgaggg gtcgcgctcg gacagggatt
     2041 tccctgggca gggagcgcat gcgattctct tgctccgcta tggggtggtc agggtcggcg
     2101 gggcagacgc gggatgccga agggcggagg tttcttccct gagcctccac cctctccgga
     2161 tatgtggaca gcccttcgtc tttcgatcgt gtgcggtcat tgccgtcgtg aggtggccgt
     2221 ctgcatttgg ttcgaggatc cccacccgct cgtccgttct gtcggtcacg gcgaccggaa
     2281 cgaacaggcc gaaaaagaca tatcgagtga gtagccgcgg aatgacgcct gtggctctcg
     2341 acactgttga tgacggattg cggcccggct gcgagctatc actgtggctg gaaaggtgga
     2401 agccttgact ctttcgacca cggtgcgggc a
//
LOCUS       DASRZS010000147         6189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_468310, whole genome shotgun sequence.
ACCESSION   DASRZS010000147 DASRZS010000000
VERSION     DASRZS010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468310"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="VFH83_03695"
     CDS             complement(<1..1369)
                     /locus_tag="VFH83_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6485497.1"
                     /translation="MSRRKVLLIIAAVLLVVALPAVAQSKVTITVYGDEGQNLTPVEW
                     LKPAAAARGITIETVGVPFTSVYEKLKTEFVGGTGAYDLVIFYPAYMGEFAEFGYLQP
                     LDPLFSIHDPNMADVVPAYRDLYDMYNGKTYALPYDGDILNLYYVKSLFNDAGEKAAF
                     KAKFGQDLKVPRTWDDMAKVAQFFTRKKGDKLAGKTLDQDFYGFAFLGARGFAYAWWS
                     NVFGSLGGAYFDRQMKPQINAPAGVQAISILKNLMPYCPPDVMSFGYTELMNAYLQGR
                     VATMVQWADVWKKANDPKFSTPFILHNAGVSQIPGVKQPDGSIYFRSAAPVGRVMAIP
                     ATSKHPKEAFWVASMLSNDESIKAVTSNDTGEDPYRISHFKNIPAFGKFGSPADAKEY
                     LDVDADNLAHIFPDINIPGSADYLDALDVAVTSALSGTADAAGALNTAAKAWDKITQN
                     LDPAKQ"
     gene            complement(1417..2514)
                     /locus_tag="VFH83_03700"
     CDS             complement(1417..2514)
                     /locus_tag="VFH83_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485498.1"
                     /translation="MGTVELSQVWKIYDNHFEAVKGITATLRDGEFISLLGPSGCGKT
                     STLRMIAGLEAISRGDIRIDGIRVNELEPGDRNIAMAFESYALYPHLTVFENIAYPLR
                     IRRTPEAEIQRQVSAICDSLGISAYRNLRPSSLSGGAQQRTGLARALVRSASLYLLDE
                     VLSHLDANERVMLRGEIRRVQKLMKLTALFVTHDQLEALAMSDRIIVMNDGELQQIGT
                     PEEIYDTPENLFVAGFVGEPAVNTFPAERIREGGRDLLSIAGQKVGLADLRLPAVPDR
                     QKFIIGIRPKHLVLGTAAAPGYLRGQVHTFEVLGDFTLAALTMGTGDLVFKVGDAGLR
                     LALDQPLTFSFDPGKVLFYDVQTTSRIRPGQ"
     gene            complement(2516..3607)
                     /locus_tag="VFH83_03705"
     CDS             complement(2516..3607)
                     /locus_tag="VFH83_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485499.1"
                     /translation="MPEYLEIRNLTKQYARVKGIDDVSFSCKEGSFIVILGPSGAGKT
                     TTLKIIAGILQETSGDVLRRGESLRSVSPQVRNVSMTFEDYALYPTFSIYENIASPLR
                     VRGVAREEVDARVKRTASLLGIDEHLDKLPSQISGGQKQRTALARCLIRDAEIYLLDE
                     PIAHLDAKLRHRMRGEFKRIHKDLGKTMIYVTHDYREALALADTVVVLNKGKVEQIGP
                     PDEVFSRPRNVFVATLLGEPPMNLVHFDEARSVGGALSLSGKDRSVALRGIEAGPAGG
                     TVTLGFRHFSAALQPQAGENCLEGSVYVSEQIEDSRVYSLMVGDDLVKVLTPRDTRFD
                     INQRVSIRLDPRAVYLFDGASGERIGAGA"
     gene            3829..4605
                     /locus_tag="VFH83_03710"
     CDS             3829..4605
                     /locus_tag="VFH83_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET6485500.1"
                     /translation="MNKPERLNAIMQTLLLTKASSVSDLSRQLKVSHMTVRRDLEVLQ
                     ESEQVKILHGNVILHPKRNARDDDSYYSLIAAGAKHPDLKRKIGQLAASLIEPEDTLI
                     IDCGSTTEYLARCLPEEISFTALSYALNIISSTVRRRNCISMFSGGVFHENTLMFESA
                     EGLEVIRHFRATKAFVSASGVSAQLGVTCMNSYERQTKREMIRSSKRKILLVDSSKFG
                     IIRADHFAELSEFDEVVTDGGIPREYVDTIQGMGITLRIA"
     gene            4639..5712
                     /locus_tag="VFH83_03715"
     CDS             4639..5712
                     /locus_tag="VFH83_03715"
                     /inference="COORDINATES: protein motif:HMM:NF025839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HET6485501.1"
                     /translation="MTGKVYLERSVFTDRQRVLLEMGELQAKSLLFSTGVPGLRIENA
                     RGHIVMLPWQGQQVWDAVFDGRRLTMQTVFPEPVPTTHLLESYGAFLYHCGALRMGSP
                     GPQDTHPLHGELPTAPYQEAWLEFGEDEGGRWIGLSGACHYARAFGDTYRAVPLVKLH
                     ENGTVLDVSMTIENRAHFPMDLMYMCHVNFLPAVNGEIIQAAGWDTKDMAVRSSIPAH
                     VKPTKQFLAFLESLSKDPSVTRFIRPEDEYNPEIVFYIRNLRRDAQGLTHMMQKHPEG
                     TSDYVAWDPQVLDHAVRWILVHADQKVMGMALPSTCDPEGYTAEKQKGNVRSIPARGS
                     VTFQVRAGCLDAAETARRISALR"
     gene            5745..>6189
                     /locus_tag="VFH83_03720"
     CDS             5745..>6189
                     /locus_tag="VFH83_03720"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011910289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HET6485502.1"
                     /translation="MSRPKITVVGSNMVDLITYIRRMPKEGETIEAPRFEMGFGGKGA
                     NQAVAAALLGADVAMVTKVGDDLFGPNTKKNLERVGVDTTHVQAVPGVSSGVAPIFVD
                     ESSHNSILIIKGANAHLLPADVDRAKDRILASDIVIMQLEVSLETV"
BASE COUNT         1127 a         1993 c         2001 g         1068 t
ORIGIN      
        1 tctgcttggc cgggtccagg ttctgggtga tcttgtccca ggccttggcc gcggtgttga
       61 gcgcgccggc cgcgtccgcc gtgcccgaga gggcgctggt gaccgcgaca tcgagcgcgt
      121 ccaggtagtc cgcggacccg gggatgttga tgtccgggaa gatgtgggca aggttgtccg
      181 cgtccacgtc caggtattcc ttggcgtccg ccggggaacc gaacttgccg aacgccggga
      241 tgttcttgaa gtgcgagatc cggtagggat cttcacccgt gtcgttcgac gtgacggcct
      301 tgatgctctc gtcgttgctg agcatggacg ccacccagaa cgcctccttg ggatgcttgg
      361 aggtggcggg gatggccatg acgcgcccca cgggggccgc ggaccggaag tagatcgagc
      421 cgtccggttg cttcacgccg gggatctgcg aaacgcccgc gttgtgcagg atgaacggcg
      481 tggagaactt cggatcgttg gccttcttcc acacgtccgc ccactgtacc atcgtggcca
      541 cgcggccctg gaggtacgcg ttcatcagct cggtgtagcc gaagctcatg acgtcgggcg
      601 ggcagtaggg catgaggttc ttcaggatgc tgatggcctg cacgccggcc ggcgcgttga
      661 tctgcggctt catctggcgg tcgaagtacg cgccgcccag ggagccgaac acgttgctcc
      721 accacgcgta ggcaaagcca cgcgcgccga ggaacgcgaa cccgtagaag tcctggtcca
      781 gcgtctttcc ggccagcttg tcgcccttct tgcgcgtgaa gaactgcgcc accttggcca
      841 tgtcatccca ggtgcggggg accttcaggt cctggccgaa cttcgccttg aacgccgcct
      901 tctcaccggc gtcgttgaac aggctcttca cgtagtacag gttcaggatg tccccgtcgt
      961 acggcagcgc gtaggtcttc ccgttgtaca tgtcgtagag gtcccggtag gccggcacca
     1021 cgtccgccat gttgggatcg tggatggaga acagcgggtc cagcggctgc aggtacccga
     1081 actcggcgaa ctcgcccatg taggccgggt agaagatcac caggtcgtag gcgccggtgc
     1141 cgccgacgaa ctccgtcttc agcttctcgt agacagacgt gaacgggacg cccaccgtct
     1201 cgatggtgat tccccgggcg gccgccgccg gcttcaacca ctcgacgggg gtcaggttct
     1261 gaccctcgtc cccgtagacg gtgatcgtga ccttgctctg ggcgacggcc ggcagggcca
     1321 ccaccaggag cacggccgcg atgatcagca ggaccttcct tctgctcata ctctccacct
     1381 ccgacagatg attctcgaag ctcgaaccac gtcgagctac tggccaggac gaatacgcga
     1441 cgtggtctgc acgtcgtaga acagcacctt cccgggatcg aaggagaacg tgagaggctg
     1501 gtccagcgcc aggcgcaggc cggcgtcccc caccttgaac acgagatctc ccgtccccat
     1561 cgtgagggcg gcaagcgtga agtcccccag gacctcgaag gtatgcacct ggccgcgcag
     1621 gtatcccggg gcggcggcgg tgcccagcac gaggtgcttg ggacggatgc cgatgatgaa
     1681 cttctggcgg tccggcacgg ccggcaggcg caggtcggcg agccccacct tctggccggc
     1741 gatggacagc aggtcgcgcc ccccctcccg gatgcgctcc gcggggaacg tgttcaccgc
     1801 cggctctccg acgaagccgg ccacgaacag gttctccggc gtgtcgtaga tctcctccgg
     1861 ggtgccgatc tgctgaagct ctccgtcgtt catgacgatg atccggtccg acatggcgag
     1921 cgcttccagc tggtcgtgcg tgacaaagag cgccgtgagc ttcatcagct tctggacgcg
     1981 gcggatctcg ccgcggagca tgacccgctc gttggcatcc aggtgcgaaa ggacctcgtc
     2041 cagcaggtac agggaggcgg accgcaccag ggcgcgcgcc aggccggtgc gctgctgcgc
     2101 gcccccgctc aaggacgacg gccgcaggtt gcggtacgcg gagatgccga gagagtcgca
     2161 gatggcgctg acctgccgct ggatctcggc ctccggcgta cggcggatcc gcaggggata
     2221 ggcaatgttc tcgaagaccg tgaggtgcgg gtacaacgcg taggactcga aggccatggc
     2281 gatgttgcgg tcccccggct ccagctcgtt cacccggatc ccgtcgatgc ggatgtcccc
     2341 gcgggagatc gcctcaaggc cggcgatcat ccgcagcgtg gacgtcttcc cgcacccgga
     2401 ggggcccagc agggagatga actccccgtc ccgcagcgtg gcggtgatgc ccttcacggc
     2461 ctcgaagtgg ttgtcgtaga tcttccagac ctgggagagc tctaccgttc ccatgtcagg
     2521 cccccgcccc gatccgctcc ccgctggcgc cgtcgaagag gtacacggcc cgcgggtcca
     2581 gccggatgga cacccgctgg ttgatgtcga aacgtgtgtc cctcggcgtg agcaccttca
     2641 caaggtcgtc tccgaccatg agggagtaca cgcgggagtc ctcgatctgc tcggagacgt
     2701 acaccgaccc ctccaggcag ttctccccgg cctgcggctg cagcgcggcg ctgaagtgcc
     2761 ggaagccgag cgtgaccgtt ccaccggcgg gacccgcctc gatcccccgc agggcaaccg
     2821 aacgatcctt gccggacagg gagagcgcac cgccgacgga ccgggcttca tcgaaatgca
     2881 cgaggttcat gggcggctcc cccagcagcg tggccacgaa cacgtttcga ggccgggaga
     2941 acacctcgtc cggcggcccg atctgctcca cctttccctt gttcagcacg accacggtgt
     3001 ccgccagcgc cagcgcctcg cggtagtcgt gtgtcacgta gatcatcgtc ttgcccaggt
     3061 ctttgtggat ccgcttgaac tcccccctca tgcggtggcg gagcttcgcg tcaaggtgag
     3121 caatcggctc gtccaggagg tagatctccg cgtcccggat caggcaccgg gcgagcgccg
     3181 tcctctgctt ctgaccgccg ctgatctggg acggcagctt gtccaggtgc tcgtcgattc
     3241 ccagcaggct cgcggtgcgt ttcacgcgcg cgtccacttc ctcccgcgcc acgccgcgga
     3301 cccggagcgg ggaagcgatg ttttcataaa tggaaaacgt cgggtacagc gcgtagtcct
     3361 cgaacgtcat ggaaacgtta cgcacctggg ggctcacgga ccgcaggctc tctccccgcc
     3421 taagcacgtc tccagaggtt tcctgcagga ttcccgcgat gatcttcagc gtcgtcgtct
     3481 tgccagcgcc ggagggtccg agaatgacga tgaagctgcc ctccttgcaa gagaacgaca
     3541 cgtcatcgat gcccttgacc ctggcatact gcttagtgag attcctgatc tcgaggtatt
     3601 ccggcatcgt ctccagtcct ttcgagtcag ccccaagcgc ttcagtgtgg gaaatcgctg
     3661 gcattatagg ttcggaaatg ttaactgtca agaaaaaaca atgccgcttg tgcagttatt
     3721 aacttcacac tataatattt taacatcctc gcgatacaag caggattctc atgctattat
     3781 ggaagtcatg acgattttga ccagaaaact gccaggaacg ggccgggcat gaacaagccc
     3841 gagcgcctca acgccatcat gcagacgctg ctgctcacca aggcatccag tgtcagcgat
     3901 ctctcgcgcc agctcaaggt cagccacatg acggtccgtc gcgacctcga ggtcctgcag
     3961 gagagtgaac aggtcaagat cctgcacgga aacgtcatcc tccaccccaa gcggaacgcc
     4021 cgggacgatg acagctacta ttccctgatc gcggcaggag ccaagcaccc ggatctgaag
     4081 cggaagatcg gccagttggc cgcctccctg atcgagccgg aagacaccct gatcatcgat
     4141 tgcgggtcca cgaccgagta cctggcgcgc tgcctgccgg aggagatcag cttcacggcg
     4201 ctgagctatg ccctgaacat catctcctcc acggtacgga ggagaaactg catatcgatg
     4261 ttctcgggcg gcgtcttcca cgagaacacg ctcatgttcg agagcgcgga aggactggag
     4321 gtgatccgtc acttccgcgc cacgaaggcg ttcgtgtcgg cctccggtgt cagcgcgcag
     4381 ctcggggtca cctgcatgaa ctcctacgag cggcagacga agcgggaaat gatccgctcc
     4441 tccaagcgca agatcctgct ggtggactct tcgaagttcg ggatcatccg ggcggaccac
     4501 ttcgccgagc tctccgagtt cgacgaggtg gtgacggacg gcggcatccc ccgggaatac
     4561 gtggacacga tccagggcat gggaatcact ctgcggatcg cctgagcggt ccgggcggca
     4621 acggaagagg agggcacgat gacgggcaag gtctacctgg agcgaagcgt gttcacggac
     4681 cggcagaggg tcctcctgga gatgggggag cttcaggcga agagcctgct gttctccacc
     4741 ggcgtgccgg gacttcgcat cgagaacgcg cgcgggcaca tcgtcatgct gccctggcag
     4801 ggccagcagg tatgggacgc cgtctttgac ggccgccggc tcaccatgca gacggtattc
     4861 cccgagccgg tgcccaccac gcacctcctg gaatcctacg gcgccttcct ctaccactgc
     4921 ggcgcgcttc gcatggggag ccccggcccc caggacacgc accccctgca tggggagctg
     4981 cccaccgcgc cctaccagga agcgtggctt gagttcggcg aggacgaggg cggacgctgg
     5041 atcggtctct cgggggcctg ccactacgcg cgggccttcg gcgacaccta ccgcgccgtg
     5101 ccgctggtga agctgcacga aaacgggacg gtcctggacg tatcgatgac catcgagaac
     5161 cgggcccatt tcccgatgga cctcatgtac atgtgccacg tgaacttcct gccggcggtg
     5221 aacggagaga tcatccaggc ggccgggtgg gacacgaagg acatggcggt gcgctccagc
     5281 atcccggcgc acgtgaagcc cacgaagcag ttcctggcgt tcctggaatc gctttccaag
     5341 gacccgtcgg tgacgcgttt catacggccg gaggacgagt acaatcccga gatcgtgttc
     5401 tacatcagga acctgcgccg cgacgcgcag gggctcaccc acatgatgca gaagcacccg
     5461 gagggaacct ccgactacgt ggcgtgggac ccgcaggtcc tggatcacgc cgttcgctgg
     5521 atcctcgttc acgcggacca gaaggtcatg ggcatggccc tgccgtccac gtgcgacccg
     5581 gagggataca ccgcggagaa gcagaaaggg aacgtgagaa gcatcccggc ccggggaagc
     5641 gtcacgttcc aggtccgggc cgggtgcctg gacgcggcgg agaccgcccg ccggatttcc
     5701 gccctgcggt agccgcgggg agtcagcgcc agggaggatg aagaatgagc agaccgaaga
     5761 tcaccgtcgt gggaagcaac atggtggacc tcatcaccta catccgtcgc atgccgaagg
     5821 aaggggagac catcgaggcc ccccggttcg agatggggtt cggtggcaag ggggccaacc
     5881 aggccgtggc cgccgcgctg ctgggagcag acgtggccat ggtcaccaag gtgggggacg
     5941 acctgttcgg gccgaacacg aagaagaacc tggaacgcgt cggcgtcgac acgacgcacg
     6001 tgcaggcggt gcccggggtt tccagcggcg tggcgcccat cttcgtggac gagagctccc
     6061 acaacagcat cctcatcatc aagggcgcca acgcgcatct cctgccggcg gacgtcgacc
     6121 gggcaaagga ccgcatcctc gccagtgaca tagtgatcat gcagctggag gtcagcctgg
     6181 agaccgtct
//
LOCUS       DASRZS010000148         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4704134, whole genome shotgun sequence.
ACCESSION   DASRZS010000148 DASRZS010000000
VERSION     DASRZS010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4704134"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1095)
                     /locus_tag="VFH83_03725"
     CDS             complement(76..1095)
                     /locus_tag="VFH83_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485503.1"
                     /translation="MGGFTLRYLFQRLLMWLLTILIGVTLVFAIPRLGKSDPTTAMLL
                     NMMNMRGYVENADQIIATYKHSFGLDAPWYVQYIRYLGHVARFDYGFSFSRFPTNAWE
                     IVKPALPWTLTLLSIAAVLSLLVGATVGALMGWSKTPRWLRNTLPISLTFTSIPYFMF
                     GILLIFLFAFRIHLFPATGGYSMDVNPGWNMPFIRSVAAHAVLPLVSIVLTSMGAWAL
                     GMRGLMIGVNNEDYFLLAQAKGLSPSRIFFRYGIRNTILPVLTAFALGLGGLVGGSTI
                     VEYVFAYPGTGYTLYRSIQEQDYTVIQTITNLMIIITATAVLVIDLLYPLIDPRITFK
                     KNVAS"
     gene            complement(1202..>2177)
                     /locus_tag="VFH83_03730"
     CDS             complement(1202..>2177)
                     /locus_tag="VFH83_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6485504.1"
                     /translation="RTAIDNGFDSMQDISVGALEAIVAQNPKWQAWFKQKPYAVPDPC
                     ARIISLNSAKQPWNDKDMRKMLSLVMDRQQIVDIAYEGSTTLAAYFWPAYNSMKPYSD
                     LVDKQVYASFLKPDTAKAASILQSKGYTKSGKYWQKDGKDLTIEIQVPEDFIELIRIG
                     DVYVEQLQKFGINATESKLGAVFYTNSSNGDYEAQSNWFACGSVNEPFTTLNQFTGAA
                     APLGQTAKGGPVDNQWRWSNKKYSDIVAQLGVLKVGDPKALELTRQALAILYDELPAF
                     PAAQSRKIVPFNYTYWTGYPTADNYYVFPCNWCNLWAVVMPKLQRAAK"
BASE COUNT          394 a          609 c          760 g          414 t
ORIGIN      
        1 gcacgccggt ggtccggggc gtgggctgcg cctggggtaa gggtctgctc gcggtcgaca
       61 tggtttctgc ctgcctcacg atgccacgtt cttcttgaac gtaatccggg gatcgatcag
      121 cgggtagagc agatcgatga cgaggacggc cgttgcggtg atgatgatca tcaggttggt
      181 gatggtctgg atgaccgtgt agtcctgctc ctggatggac cggtacagag tgtagccggt
      241 tcccggatag gcaaagacgt actccacgat ggtggaccct cccaccagcc cgcccagccc
      301 cagcgcgaag gcggtcagga ccgggaggat ggtgttgcga atgccgtagc ggaagaagat
      361 ccgtgatggc gagagtccct tggcctgggc cagcaggaag tagtcctcgt tgttgacccc
      421 gatcatgagc ccccgcatgc ccagggccca ggcccccatg gacgtgagca cgatggagac
      481 gagcggcagc accgcgtggg cggccacgct gcggataaac ggcatgttcc agccggggtt
      541 cacgtccatg gagtaaccgc cggtcgctgg aaacaggtgg atgcggaacg cgaagaggaa
      601 gatgagcagg atcccgaaca tgaagtacgg gatcgacgtg aacgtcaggc tgatcggcag
      661 ggtgttgcga agccaccgcg gcgtcttgct ccaccccatg agcgcgccga ccgttgcgcc
      721 gaccaggagg gacaggaccg ccgctatgga gagcagcgtc agcgtccagg gcagggccgg
      781 cttgacgatc tcccaggcgt tggtcgggaa acggctgaag gagaagccgt agtcgaaccg
      841 ggccacgtga cccaggtagc ggatgtactg gacgtaccag ggagcgtcga gcccgaagct
      901 gtgcttgtag gtggcgatga tctggtcggc gttctccacg tacccgcgca tgttcatcat
      961 gttgaggagc atggcggtgg tgggatcgct ctttcccaga cgcgggatgg cgaacaccag
     1021 ggtgaccccg atcaggatcg tgagcagcca catcaggagc cgctggaaaa gataccgaag
     1081 ggtgaagccg cccatgagtg ttctcccgtg aaggggttag tgctttgaga aggctctgat
     1141 ggcccccggg aggggagcca tcagaggatc atggaaaggt tgtttcacga agacctggat
     1201 gctacttcgc cgccctctgg agcttgggca tgacgaccgc ccacaggttg caccagttgc
     1261 acgggaacac gtagtagttg tccgcggtcg ggtagccggt ccagtacgtg tagttgaacg
     1321 gtacaatctt gcgcgactga gccgcgggga acgccggcag ctcgtcgtag agaatggcca
     1381 gggcctggcg ggtcagctcc agcgccttgg gatcaccgac cttgagcacg ccgagctgcg
     1441 ccacgatgtc gctgtacttc ttgttgctcc agcgccactg gttgtccacc ggtccgccct
     1501 tggcggtctg gcccaggggg gcggccgcgc cggtgaactg gttcagcgtg gtgaacggct
     1561 cgttgacgga gccgcaggca aaccagttgg actgcgcctc gtagtcgccg ttggaggagt
     1621 tggtgtagaa caccgcgccc agcttggact ccgtggcgtt gatcccgaac ttctggagct
     1681 gctcgacgta gacgtcaccg atgcggatga gctcgatgaa gtcctcgggg acctggatct
     1741 cgatggtgag gtccttgccg tccttctgcc agtacttgcc gctcttggtg tagcccttgg
     1801 actgcaggat ggaggctgcc ttggcggtgt ccggcttcag gaagctggcg tagacctgct
     1861 tgtccaccag gtcggagtac ggcttcatgg agttgtaggc cggccagaag tacgcggcca
     1921 gggtggtgga gccttcatag gcgatgtcca cgatctgctg gcggtccatc accaggctca
     1981 gcatcttgcg catgtccttg tcgttccagg gctgcttcgc cgagttcagg gagatgatgc
     2041 gcgcgcaggg atcggggacc gcgtacggct tctgcttgaa ccacgcctgc cacttggggt
     2101 tctgggcgac aatggcctcc agcgcgccca cggaaatgtc ctgcatgctg tcaaagccgt
     2161 tgtcgatggc ggtgcgg
//
LOCUS       DASRZS010000149         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5471341, whole genome shotgun sequence.
ACCESSION   DASRZS010000149 DASRZS010000000
VERSION     DASRZS010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5471341"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..1652)
                     /locus_tag="VFH83_03735"
     CDS             complement(246..1652)
                     /locus_tag="VFH83_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6485505.1"
                     /translation="MKLHNPERPVLMVDDEPTFLEIMAAGLERVGITNVVRITDSSEA
                     RRLIEAQEFSAITLDLYMPGITGFDLLPVALQAWPATPVIMVSSADKLDVAVGCMRDG
                     AFDYILKPVDHHRLTTSVSHALERWETSREITSLKEHLISGSLAHPEAFAGIVTGDPA
                     MVGLFRYAEAIAPTSLPVLVTGETGVGKELMARAIHALSGRKGSFLAVNVAGLDDALF
                     ADTLFGHLKGAFTSADSTREGMVAKAERGTLFLDEIGDLAPESQIKLLRLLQEGEYYP
                     LGSDRPSASSARYIFATNIDLAQATKERRFRQDLMYRLGSHRMAIPPLRERRGDLPLL
                     VEHILDKAARTIPMEKPKAPDRLFAILRGYGFPGNVRELEGILMDAVVRGKDGGVSLE
                     SVRSAVGAETAGIPDGSVSPEPGGNLFSSMPTLPTIKQAISVLVDEALLRSGGNQTQA
                     AALLGISRPALTKRLSRR"
     gene            1884..>2754
                     /locus_tag="VFH83_03740"
     CDS             1884..>2754
                     /locus_tag="VFH83_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6485506.1"
                     /translation="MGNLSVQTVGVFVAQLDDAYQIAVWRGIESRARELGVGIVCFVG
                     HRIDSPIPSEAACNVAYRLADRRTIDALVVISSAIATSFNDEVIARLFPPRGLPQVSV
                     GLKVPEVPSVTVDGCEGMRAVVSHLIQSHGKSRFALIRGPAGHPEAEQRERAVRSTLH
                     DCGLELDEAMTADGSFLRSLGRRAASDLLARGRPFDALVCVNDLMALGAMEVLREAAI
                     RVPQDVAVVGFDGIEEGRYVTPPLTTVMQPLDALGRSAIDCAVEVMQSRVPQDRVLTG
                     SPVIRQSCGCAPKQ"
BASE COUNT          445 a          912 c          912 g          485 t
ORIGIN      
        1 tacgatggtc aacggcacca ccagaaccag gagccctacc aggaccaatc gtgttccgat
       61 ccgcatgttt ctcagcatcg ctatctcctt ctattcttgt ccctggaaga gcaggatcga
      121 tgccaagcta cgaaaaacac gctaagtcat tatccagtaa tggtttagga agcaagacgg
      181 catccgatta cggatcgagg ccggtaactt ttgtcctgga acgtcagcaa tgtcccggaa
      241 gcctgctacc gacggctcag ccgctttgtg agcgcggggc gggagatccc gagcagcgcg
      301 gcagcctgcg tctgatttcc gccgctgcgc agcagagcct catccaccag aacagaaatc
      361 gcttgcttga tcgtgggcag cgtaggcatg gagctgaaca ggtttccccc aggctcggga
      421 gaaacggagc cgtcggggat gccggcggtt tccgcgccga ccgcggatcg cacgctctcc
      481 agggaaacgc cgccgtcttt gccgcggacg accgcatcca tgagaatccc ttcgagctcg
      541 cggacgtttc cggggaagcc gtagcctcga aggatcgcga agagccggtc cggcgccttc
      601 ggcttctcca tgggtatcgt cctcgcggcc ttgtccagga tgtgttccac gaggaggggg
      661 agatctcccc gcctctcgcg aagcggaggg atggccatgc ggtgggatcc cagccggtac
      721 atcaggtcct gccggaaccg cctctccttc gtggcctggg ccagatcgat attcgtcgcg
      781 aagatgtacc gcgcgctcga ggcgctgggg cggtccgacc ccaggggata gtactcccct
      841 tcctggagca gccgcagaag cttgatctgg gactccggtg ccaggtctcc gatctcgtcg
      901 aggaagagcg tcccgcgctc cgcctttgcc accatcccct cccgggtcga gtctgctgac
      961 gtgaaggcac ccttgaggtg accgaacagg gtgtccgcga acagggcgtc gtccaggccg
     1021 gcgacgttca cggccaggaa cgaccccttg cgtccgctca aggcatggat ggcccgcgcc
     1081 atgagctcct tgccgacccc agtctccccg gtgacgagca ccggcaggga ggtgggcgcg
     1141 atagcctccg cgtagcgaaa gagcccgacc atggcggggt cccccgtgac gatgccggcg
     1201 aacgcctcgg ggtgcgccag ggagcccgag atgaggtgct ccttgaggga cgtgatctcc
     1261 cgcgaggtct cccagcgctc cagcgcgtgg ctgacgctgg tggtgagccg atggtgatcc
     1321 acgggcttca ggatgtagtc gaaggccccg tcccgcatgc agccaacggc gacgtcgagc
     1381 ttgtccgcgg aagacaccat gatgaccggc gtcgcgggcc acgcctgcag cgccaccggc
     1441 aggaggtcga agccggtgat gccgggcatg tagaggtcca gggtgatcgc ggagaactcc
     1501 tgggcctcga tgagccgccg agcctcggag ctatcggtga tccgcaccac gttcgtgatg
     1561 cccacccgct ccagtcctgc ggccatgatc tcgagaaagg tgggctcgtc gtccaccatg
     1621 agcacggggc gctccgggtt gtgcaacttc atctggctac ccttcgggga tccgttcttc
     1681 cctgcacgaa aagcttaccc ctgatgtcgg ccgccgtcat ttgggcggcc attcggggcg
     1741 ccttttcagc ggccttgctg gcggcccggc gggcggaccc atcggcgctt tgaccgatag
     1801 cgggccagtc tgctgcccgc tttgtcctgc cgatcttccg ccggtttcat cgggacggaa
     1861 aaagcgctac gcttccccag cccatgggga acctctcggt tcagacggtg ggcgtctttg
     1921 tcgctcagct cgacgacgcc taccagatag cggtatggcg aggcatcgaa tcccgcgcgc
     1981 gggagctggg ggtcgggatc gtctgctttg tggggcaccg catcgactcc cccatcccct
     2041 ccgaggccgc ctgcaacgtg gcttaccggc tggccgaccg ccgcaccatc gacgccctgg
     2101 tcgtgatctc ctccgccatc gccacttcct tcaatgatga ggtgattgcc cggctgtttc
     2161 ctccccgcgg cctgccgcag gtctccgtgg ggctcaaagt gccggaggtt ccgagcgtca
     2221 ccgtggacgg ctgtgagggc atgcgcgccg tcgtgagcca cctgatccag agccacggca
     2281 aatcccgatt cgcgctgatc cggggcccgg cggggcaccc cgaagcggag cagcgcgagc
     2341 gggccgtccg gtcaacgctc cacgactgcg gactcgagct cgatgaggcc atgaccgcgg
     2401 atggctcgtt ccttcggtcc ctgggacgcc gagcggcgtc ggacctcctg gcccggggaa
     2461 ggccgtttga cgccctggtc tgcgtgaacg acctgatggc gctgggggcc atggaggtcc
     2521 tgcgcgaagc cgcgatccgc gtgccgcaag acgtggccgt ggtgggcttc gacgggatcg
     2581 aggagggccg gtacgtcact ccgccgctca ccacggtgat gcagccgctg gatgcgttgg
     2641 ggcgctccgc catcgactgc gccgtggagg tgatgcagag ccgggtcccg caggaccggg
     2701 tgctgaccgg cagccccgtg atccgccagt cctgcggctg cgcgcccaag cagc
//
LOCUS       DASRZS010000150         5345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774 k141_83485,
            whole genome shotgun sequence.
ACCESSION   DASRZS010000150 DASRZS010000000
VERSION     DASRZS010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83485"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFH83_03745"
     CDS             <1..493
                     /locus_tag="VFH83_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6485507.1"
                     /translation="GMRKRRSGLIVNISSIGGLMGLPFQSTYSASKFGIEGFSESLQI
                     EVRDFGIRVVVVEPGDIATEVTVHRRITRGIAADSPYRKNLDDAMRSQSTNEINGWDV
                     ERVARFIGRIVQSRSPRFRYRPGPFMEVISPTFRRVMPDRLFLKVLASFSGIKPAGRQ
                     APA"
     gene            700..1359
                     /locus_tag="VFH83_03750"
     CDS             700..1359
                     /locus_tag="VFH83_03750"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET6485508.1"
                     /translation="MSLRINDTAPDFTADTTQGKIQFHQWIGDGWAILFSHPKDFTPV
                     CTTELGYMAGLEGEFKKRNCKIIGLSVDPVQSHSKWAKDIEETQGHKVEYPMIGDPDL
                     HVAKLYDMLPADAGTTSEGRTAATNATVRTVFVIGPDKKIKLMLVYPMSTGRNFNEVL
                     RVLDSIQLSARHKVSTPVNWKNGEDVIIAGSVTDEEAKKLYPTGWKAPRPYLRIVPQP
                     K"
     gene            1361..2293
                     /locus_tag="VFH83_03755"
     CDS             1361..2293
                     /locus_tag="VFH83_03755"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET6485509.1"
                     /translation="MSGLETARALGQSPVGSIGNTPLLRLTRVAAALPAGVRLLAKAE
                     WMNPGGSVKDRPAAFMILDGERRGALSPRKTIIDATSGNTGLAYAMIGAAQGYRVKLC
                     VPRNTDPGLLGTLRAYGAELVLTDAAAGMDGASREVQRLVAASPADYFFPDQLNNEAN
                     WKSHYEGTANEIWKQTEGAVTHFVAGVGTGGTLVGTGRRLRELNPASRIVCVQPSSPL
                     HGLEGLRHLPTTIVPRIYDPTLVDETVAVQTEAAQAMAVRLAREEGVFVGVSAGAAVV
                     AALEVASRLQSGVVVTVLPEGGRRFLGQAFWGNV"
     gene            2304..2753
                     /locus_tag="VFH83_03760"
     CDS             2304..2753
                     /locus_tag="VFH83_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET6485510.1"
                     /translation="MKVIVPDAIYQAMRRHGEETYPHECCGFLLGHSRDGEQRIVDAM
                     RQSNERTDSPQNRFRIAPEEFRAAEKAARATGLTLVGIYHSHPDSPARPSEFDREHAW
                     PWFSYIIVSVTGGAAAEANAWQLRDDRSGYERMALEIENGALRGESG"
     gene            2750..4177
                     /locus_tag="VFH83_03765"
     CDS             2750..4177
                     /locus_tag="VFH83_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HET6485511.1"
                     /translation="MTRVEIPTALRGFAGNRDAVDLDGGTVGELLQQLGAQYPQLKKH
                     LFDDEGKLRNFVNVYVNDDDIKHLDKEQTRVSAKDVISIIPAVAGGAANGALTQEQVQ
                     RYSRHLIMPEVAMEGQQKLRHARVLVIGAGGLGSPVLSYLAAAGVGTIGLVDFDIVDF
                     TNLQRQIIYSTSDVGRPKLEAAAERIRALNPDVQLQVHDARLGSDNALTILKGYDLVI
                     DGTDNYPTRYLVNDACVMLGIPNVYGSIFRFEGQATVFCEKDGPCYRCVYPEPPPPGL
                     VPSCAEGGVLGVLPGIIGTIQATEAIKLIIGKGTSLSGRLLVLDAMKMRFRELKLRKD
                     PNCPVCGEHPTVTELIDYEQFCGIRGEEMEAKDLGKEWEISPPALKDKLRSGEGLVIL
                     DVRNPEEAQISRIDGSILIPLGELPQRVSELSTADQIVVHCRMGGRSAKAVEFLRSVG
                     FRKVKNLVGGINAWAEQVDPSMARY"
     gene            complement(4174..>5345)
                     /locus_tag="VFH83_03770"
     CDS             complement(4174..>5345)
                     /locus_tag="VFH83_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET6485512.1"
                     /translation="RIMDRLAQQMKRRGPSLSSRVADRIAAAVSAGPGGEPSPYRAPP
                     DSGSADRVAEELCRLEGGSRALLYCSGMTAINRTLDVLRRPGRSQVIAIGHLYNDTFQ
                     GLVESQGPDEQNLFLGVDEIDTLDARIGARTAAILTETITNPLSDVPDLIELSRIANA
                     HGVPLVVDNTLATPVNCRPLTLGADYVVHSTTKYLNGRNDHGGGAVIVKDPRLVAELE
                     RRQSALDDRMSALEAGALEGNLRSVPQRMDRYHANAARVSALLAEHPAVGRLYFNGHP
                     SHRCYQTARRLLNGPGSVISFTLARADVEAFRAFYDSPLAGILKAPSLGSDLTLLCPY
                     TLLTHSKETDEQLHAIGLPRYLARIAVGCEQDIAPVLESLDAALRRSLMVPEIPA"
BASE COUNT          938 a         1711 c         1857 g          839 t
ORIGIN      
        1 cgggatgcgc aagcgtcgat cgggtctcat cgtcaacatc tcctccatcg gcggtctgat
       61 gggactcccc ttccagtcga cctacagcgc cagcaagttc ggcatcgagg ggttctcgga
      121 gtccttgcag atcgaggtga gggacttcgg catccgggtg gtggtggtgg agccggggga
      181 catcgccacg gaggtcacgg tgcaccgacg gatcaccagg ggcatagccg ccgactctcc
      241 gtatcgcaaa aacctggacg acgccatgcg ctcccagtcg accaacgaga tcaacggctg
      301 ggacgtcgag cgcgtggccc ggttcatcgg aaggatcgtc cagagccggt ccccccggtt
      361 ccgctaccgg ccaggaccgt tcatggaggt aatcagccca accttccgcc gggtgatgcc
      421 ggaccgcctg ttcctgaagg tgctggccag cttcagcggg atcaaaccgg ccggcaggca
      481 ggctcccgcg tagacggcag ccggggtcct ggcacacccc ggcatgcggc gtcaccggct
      541 tcttctattt cgcttgaaaa ccctcccctc cctttatacc ttactaatca ggtcggaata
      601 gtagacaaga cccgagcacc agagaaaggt gcttgaaacg atacgtcggg cgcctccgcg
      661 ggtgagatcc cagaggagcg cccctggaga ggagggcgca tgtccctacg catcaacgat
      721 acggctcccg atttcacggc ggatacgacc caaggcaaga tccagttcca ccagtggatc
      781 ggagacggct gggccattct cttttcccac cccaaggact tcacgccggt ctgcaccacg
      841 gagctgggct atatggcggg tctggagggc gagttcaaga agcgcaactg caagatcatc
      901 ggcctgagcg ttgaccccgt ccagagccat tccaagtggg ccaaggacat cgaggagacc
      961 cagggccaca aggtggagta cccgatgatc ggcgatcccg acctgcatgt agccaagctt
     1021 tacgacatgc tgccggcgga cgcgggcacc acctcggaag ggcggaccgc ggccaccaac
     1081 gccacggtgc ggactgtgtt cgtgatcggc ccggataaaa agatcaagct gatgctggtc
     1141 taccccatga gcacgggaag aaacttcaac gaggtgctcc gcgtgctgga ctccatccag
     1201 ctctccgcca ggcacaaggt gtccacgccg gtgaactgga agaacgggga ggatgtcatt
     1261 atcgccggct ccgtcaccga cgaggaggcc aagaagctct accccaccgg ttggaaggcg
     1321 ccgaggccat acctgcggat cgttccccag ccgaagtagc ttgagcggtc ttgaaaccgc
     1381 gcgggcgctg ggtcagtccc cggttggaag catcggcaac acgccgctcc tccgcctgac
     1441 gcgggtagcg gcagcgcttc cggccggggt acggctcctg gccaaggcgg agtggatgaa
     1501 cccgggggga tccgtgaagg accgacccgc cgcgttcatg atcctggacg gagagcggcg
     1561 cggggcgctc tcgccgcgaa agacgatcat tgacgccacc tcggggaaca ccggtctggc
     1621 ctatgcgatg atcggcgccg cccaggggta ccgggtgaag ctctgcgtgc cgcggaacac
     1681 ggaccccggg ctcctgggca cgctccgagc ctacggcgcg gagctggtgc tgacggatgc
     1741 ggcggcaggg atggacggcg caagccggga agtgcagcgg ctggtggcgg catcgccggc
     1801 tgactacttc ttcccggacc agctcaacaa cgaggccaac tggaagtcgc actacgaggg
     1861 cacggcgaac gagatctgga agcagacgga gggcgccgtg acgcacttcg tggccggcgt
     1921 gggcaccggc ggcacgctgg tgggtacggg tcggcggctg cgggagctca atcccgcgtc
     1981 gcggatcgtc tgcgtgcagc cgtcctcccc ccttcacggc ctggaggggc tgcgccacct
     2041 gcccaccacc atcgtgccga ggatctacga tccgaccctg gtggacgaga cggtggccgt
     2101 gcagaccgaa gcggcccagg ccatggcggt acggctggcg cgcgaggaag gggtctttgt
     2161 gggcgtgtcc gcgggggccg cggtggtggc cgcgctggag gttgcctccc gcctccagag
     2221 cggcgtggtg gtgacggtgc tgccggaagg cggccgtcgg ttcctgggtc aggcgttctg
     2281 ggggaacgtg tgaaggggat cccgtgaaag tgatcgtgcc ggatgccatc taccaggcga
     2341 tgcgtcgcca cggcgaggag acctatccgc acgagtgctg cggctttctg ctggggcact
     2401 cgcgggacgg ggaacagcgg atcgtggatg cgatgcggca gtccaacgag cggaccgatt
     2461 cgccacagaa ccggtttcgg atcgcgccgg aggagttccg cgccgcggag aaggcggcgc
     2521 gcgccacggg tctgacgctc gtcgggatct accattccca cccggacagc cccgcgcggc
     2581 cctcggagtt cgatcgcgag cacgcgtggc cgtggttcag ctacatcatc gtctccgtga
     2641 cgggtggagc ggccgcggag gccaacgcgt ggcagcttcg ggacgaccgc tcgggatacg
     2701 agcggatggc gctggagatc gagaacggcg ccctgagagg agaaagcgga tgactcgtgt
     2761 ggagataccg accgcgttgc gcggctttgc cggcaaccgc gacgccgtgg acctcgacgg
     2821 agggaccgtg ggggagctgc tccaacagct cggagcgcaa tacccgcagc tcaagaagca
     2881 tctcttcgac gacgagggga agctgcgcaa cttcgtgaac gtctacgtga acgatgacga
     2941 catcaagcac ctggacaagg agcagacccg ggtcagcgcg aaggacgtga tcagcatcat
     3001 cccggcggtg gccgggggcg ccgcgaacgg cgcccttacg caggaacagg tgcagcgcta
     3061 cagccggcac ctcatcatgc cggaggtggc catggagggc cagcagaagc tgcgccacgc
     3121 ccgggtcctg gtgatcgggg ccggcgggct gggctctccg gtcctctcct acctggcggc
     3181 ggcgggtgtc gggaccatcg ggctggtgga cttcgacatt gtggatttca ccaacctcca
     3241 gcgccagatc atctactcaa cctcggacgt gggtagaccc aagctggagg ccgccgcgga
     3301 acggatccgg gcgctgaatc cggacgtgca gcttcaggtg cacgatgccc ggctcggctc
     3361 ggacaacgcg ttgaccatcc tgaagggcta cgacctggtg atcgacggga cggacaacta
     3421 tcccacccgg tacctggtca acgacgcctg cgtgatgctc ggcatcccca acgtctacgg
     3481 aagcatcttc cggttcgagg gccaggcgac cgtgttctgc gagaaggatg gtccctgcta
     3541 ccggtgcgtc taccctgagc cgccgccccc ggggctcgtg ccgagctgcg cggagggcgg
     3601 agtgttgggc gtcctgcccg gcatcatcgg gaccatccag gccaccgagg ccatcaagct
     3661 catcatcggc aagggaacct cgctctcggg gcgcctcctg gtcctggatg ccatgaagat
     3721 gcgcttccgc gagttgaagc tgcgtaaaga ccccaactgc ccggtctgcg gagagcatcc
     3781 caccgtgacg gagctgatcg actacgagca gttctgcggc atccgcggcg aggagatgga
     3841 agccaaggac ctgggcaagg aatgggagat cagcccgccc gcgctcaagg acaagcttcg
     3901 ctcgggtgag gggctggtca tcctggacgt gcgcaatccc gaggaagccc agatctcccg
     3961 gatcgacgga agcatcctca tcccgctggg cgagctcccg cagcgcgttt cggagctgag
     4021 cacggcggac cagatcgtgg tccactgccg catgggcggc aggagcgcca aggcggtgga
     4081 gttcctccgt tcggtcgggt tccgcaaggt gaagaacctg gtgggcggga tcaacgcctg
     4141 ggcggagcag gtggacccgt cgatggctcg gtactaggcg gggatctccg gtaccatgag
     4201 agaccgccgc agagcggcgt ccagggattc caggaccggc gcaatgtcct gctcgcatcc
     4261 cacggcgatc cgggccaggt agcggggcaa cccgatggcg tggagctgct cgtccgtttc
     4321 cttggagtgc gtgagcagcg tgtacggaca caggagcgtc agatcggatc ccaggctggg
     4381 agccttgagg atgcccgcca gcggcgaatc gtagaacgcg cggaacgcct ccacgtccgc
     4441 gcgggccagc gtgaagctga tgacgctgcc gggcccgttg aggagccgcc gcgccgtctg
     4501 gtagcaacgg tgcgaggggt ggccgttgaa gtacagtctc cccaccgccg ggtgctcggc
     4561 caggagcgcg gatacccggg cggcgttggc gtggtagcgg tccatccgct gcggcacgct
     4621 gcgcaggttt ccctcgagag cccccgcttc cagcgcggac atccggtcat ccagggcgga
     4681 ctgccttcgc tccagctccg cgaccagccg cgggtccttg acgatcacgg cccccccgcc
     4741 gtggtcgttg cggccgttca gatacttggt cgtgctgtgc accacgtagt ccgcgcccag
     4801 ggtgagcggc cggcagttca cgggcgtggc cagcgtgttg tccaccacca gcggcacccc
     4861 atgcgcgttg gcgatccgtg agagctcgat gaggtccggc acgtcgctca gcgggttggt
     4921 gatggtctcc gtgaggatcg ccgcggtccg ggccccgatc cgggcgtcca gcgtgtcgat
     4981 ctcatcgacc cccaggaaga ggttttgctc gtccggaccc tggctctcca ccaggccctg
     5041 gaacgtgtcg ttgtacaggt gtccgatggc gatgacctgc gaccggcccg gacgccgcag
     5101 gacgtcgagg gtccggttga tcgcggtcat tcccgaacag tacagaagag cgcggctccc
     5161 accctccaga cggcagagct cttccgccac ccgatccgcc gaaccactgt ccggcggagc
     5221 ccgataggga gacggctccc cgcccggacc ggcgctgacg gcggcggcaa tgcggtccgc
     5281 cactcggctg gacaggctgg ggccgcgtcg tttcatctgc tgggccagcc ggtccatgat
     5341 ccggt
//
LOCUS       DASRZS010000151         4190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5472236, whole genome shotgun sequence.
ACCESSION   DASRZS010000151 DASRZS010000000
VERSION     DASRZS010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5472236"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VFH83_03775"
     CDS             <1..634
                     /locus_tag="VFH83_03775"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HET6485513.1"
                     /translation="TSIDTVFRMVREAGIQARIGVTEWNVTGGWWGNDRGKLLTLETA
                     LFAGRYLNLLHRRADVVGLACRSNLTNSMASGMIQTSAAGLYLTPGYHVMRLYATHSQ
                     PVALTISDPPEGVDASACASEDGTRLTIFVVNMKADPVDMPLDLSDLGPGLAPDSAEV
                     VCDTLDRRQPDLINHPSAPDRVRTVALTLHGNAVTLPAYSAAAIECGGDE"
     gene            631..870
                     /locus_tag="VFH83_03780"
     CDS             631..870
                     /locus_tag="VFH83_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485514.1"
                     /translation="MTTYRIATLAVCFLALAARARAEPPATADGLPAFPGSVLQSDDK
                     SQLGPMTRLDRKYSTARPAREVLDFYTEALGLRSP"
     gene            complement(878..1399)
                     /locus_tag="VFH83_03785"
     CDS             complement(878..1399)
                     /locus_tag="VFH83_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485515.1"
                     /translation="MVLAAAVILGAVTAFYFLFFFFFPLGRGAVYFPSSRAHTSAMVE
                     LADPRHGEKAVDLGSGDGRVVIALARAGAEAHGFEINPALVLLSRWNVRRSGLARQAR
                     IHWGSFWRADLSPFHVVTVFQGGFVMRRLERKVLKELRPEARLVSDYWGLPNLKPQSL
                     SGTLFLYRVSDTR"
     gene            complement(1423..2454)
                     /locus_tag="VFH83_03790"
     CDS             complement(1423..2454)
                     /locus_tag="VFH83_03790"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HET6485516.1"
                     /translation="MTETSSYAMGAVPGYRSTRPGIQIGIQLGPDASPDDMRFARQLG
                     VEWVMTSLPEGAEGTEEEYRAVCGRFASEGLKVYRLANHRCHNMEAVTLALENRDAKI
                     AEYLTYLRNLSRVGVRYATYAHMANGIWSTESEPIRGDAVGRAFRLGKPSQGWWIRET
                     YRGQLTHGRRYSEDELWANYEYFIRKVVPVAEGEGIRIGFHPDDPPVYELGGIPRCIF
                     GSFAGYQRALEIAGSPNVGVCLCCGCWLEGGTAMGADLLEAIRAFGSQGKLFKVHFRN
                     VTAPLPEGFVETYLDDGYMDMARVMRALRDVGFDGCVISDHGQTMVGGRYASEAFAIG
                     FMKGIQRSL"
     gene            2529..3782
                     /locus_tag="VFH83_03795"
     CDS             2529..3782
                     /locus_tag="VFH83_03795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6485517.1"
                     /translation="MQPASRRVRKRSGSFNKKGALGLIFVIMLMDIIGITILNPVAPY
                     LVRRYDERALMVTMITIIYAVGQFVAAPLMGKLGDRLGRRPVLLVSIAGQGAGYLIFA
                     LGGSLWVLLAARLVGGITGGNLSTAAAYIADVSAPQERSKNFAMIGTAWSLGLILGPA
                     AGGLMGTLALEAPAYIAAVLSFVNVGLGFFLLQESLPRERRETTPLRGADLNPIVAIV
                     DMGRKPGLGWLLIVMSLFNFAFNGINAVGVLFVIDKFHAATWQVSVMLMLGGGTIAVC
                     NSLLVPRWVPRFGERVTGAVSLLGMAVFDVAIFLVPLLWLVILISMAVSAMSSFTFPT
                     LTTLSTDRVGHSEVGLLLGVTTAIGSLMNILGPLWAGAIYDHVMVGSPYWMGAAVFVT
                     AGLMLLRPATIDRGGRKLYRPVTER"
     gene            3792..>4190
                     /locus_tag="VFH83_03800"
     CDS             3792..>4190
                     /locus_tag="VFH83_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HET6485518.1"
                     /translation="MASQKIPSPLASWYPSPLRSWYRAPTRVVWQSDAGVTHASELLR
                     SGPAQATLEPPFPPCTLTRAAEGAGGLLLDFGTELQGFVELITPISQDKRPGKVRVRF
                     GESASEAMAELGDPGNAGNDHAMRDQVVELP"
BASE COUNT          677 a         1447 c         1382 g          684 t
ORIGIN      
        1 aacgagcatc gacacggtat tccggatggt cagggaggcc gggatccagg cgaggatcgg
       61 cgtcacggag tggaacgtga cgggcggctg gtggggcaac gaccgcggga agctcctgac
      121 gctggagacc gcgctcttcg ccggccgcta cctgaacctc ctccaccgcc gggcggacgt
      181 cgtgggtctg gcgtgccgat ccaacttgac caacagcatg gcctccggaa tgatccagac
      241 gagcgcggcc ggcctgtacc tcacgcctgg ctatcacgtg atgagactct atgccacgca
      301 ctcccagcct gtggcgctca cgatctccga tccacccgag ggagtagatg catccgcgtg
      361 cgcttccgag gatgggacgc ggctcacgat cttcgtggtg aacatgaagg cggatccggt
      421 cgatatgccg ctggatctgt ccgacctggg ccctgggttg gcgcccgaca gcgccgaggt
      481 ggtctgcgac accctcgatc ggcggcagcc ggacctgatc aatcacccga gcgcgccgga
      541 ccgcgtacgg acggtagcgc tgacgctcca cggcaatgcg gtcacgctgc ccgcctactc
      601 ggcggcggcc atcgaatgcg gaggagacga atgacaacgt ataggatcgc tacgctggct
      661 gtctgcttcc tggccctcgc tgcccgcgca cgtgccgagc ctccggctac cgccgatggc
      721 ctacccgcgt tcccaggctc cgtgcttcaa tcggacgaca agagccagct cggcccgatg
      781 acccgtctgg accgcaagta ctccacggcg aggccggcca gagaggtcct cgacttctat
      841 acggaggccc tcggcctaag gtccccgtag gaggatctca ccgggtgtcg gacacccggt
      901 agaggaagag ggtgccggaa agggactggg gcttcaggtt cggcaaaccc cagtagtcgg
      961 aaaccaggcg cgcttcgggg cggagctcct tcaggacctt cctctcgagc ctccgcatga
     1021 cgaagcctcc ctggaagacc gtcaccacgt ggaacgggga caggtccgcg cgccagaagc
     1081 tgccccaatg gatgcgcgcc tgacgcgcaa ggccggacct gcgcacgttc catcgcgata
     1141 gaagcacgag cgcgggattg atctcgaacc cgtgggcctc ggcgccggcg cgcgccagcg
     1201 cgatcaccac gcggccatcc ccggagccca ggtccaccgc cttctctccg tggcggggat
     1261 cggcaagctc gaccatggcg gaggtatggg cgcggctgct ggggaagtag acggcgccgc
     1321 gacccagcgg gaagaagaaa aagaacagga agtagaacgc cgtgacggcg cccagaatca
     1381 cggcggcggc aagcaccatc gcgagtcccc tcggccttcc ccctacaggc tgcgctggat
     1441 gcccttcatg aacccaatgg cgaaggcctc actcgcgtag cggccgccta ccatggtctg
     1501 gccgtggtcg gagatgacgc agccgtcgaa ccccacgtcc ctcagcgccc gcatcacgcg
     1561 ggccatgtcc atgtacccgt catccaggta cgtttcgaca aacccctccg ggaggggcgc
     1621 ggtgacgttc cggaaatgga ccttgaagag cttcccctgg gacccgaaag cgcggatggc
     1681 ctccaggagg tccgcgccca tcgccgttcc gccttccagc cagcagccgc agcacagaca
     1741 cacgccgacg ttcggactgc cagcgatctc cagggcgcgc tggtaaccgg cgaagctccc
     1801 gaagatgcat cgagggatcc cgccgagctc gtagacgggc ggatcgtccg gatggaatcc
     1861 gatgcgtatc ccctccccct ccgccacggg gaccaccttg cggatgaagt actcgtagtt
     1921 cgcccagagc tcgtcctccg agtagcgccg accgtgggtg agctggcccc ggtaggtctc
     1981 acggatccac cacccctggc tgggtttgcc aaggcggaac gcccggccca cggcgtcccc
     2041 tcggatgggc tcggactccg tgctccagat cccgttggcc atatgcgcgt aggtggcgta
     2101 acgaacgccg acccgagaga ggttgcggag gtaggtcaga tactcggcga tcttggcgtc
     2161 ccggttctcc agggccagcg tcaccgcctc catgttgtgg cagcgatgat tggccagccg
     2221 gtagaccttg aggccctcgg aggcgaaccg gccgcacacc gcgcggtact cctcctccgt
     2281 tccttccgcc ccttcgggaa ggctggtcat gacccattcc accccgagct gtctcgcgaa
     2341 gcgcatgtca tccgggctcg cgtccggccc gagctggatc ccgatctgga tccccggccg
     2401 cgtggaccgg tacccgggaa cggcgcccat cgcgtaagaa gacgtctccg tcatcgaggc
     2461 ccccctggga ccggaaggta ccctgccgga gcccgcccgg gaagagactc tgatgtataa
     2521 tgccgcccat gcagccggca tctcgaaggg tgcgcaagcg ctccgggtcg ttcaacaaga
     2581 agggcgcgct cggtctcatc ttcgtgatca tgctcatgga catcatcggg atcaccatcc
     2641 tcaacccggt ggcgccctac ctggtccgcc gctacgacga gcgcgccctg atggtgacga
     2701 tgatcaccat catctacgcg gtggggcagt tcgtggcagc gccgctgatg ggcaagctgg
     2761 gtgaccgcct ggggcgcagg ccggtgctgc tcgtgagcat cgccggacag ggcgccggct
     2821 atctcatctt cgcgctgggc ggctccctgt gggtcctcct ggcggcgcgc ctggtgggcg
     2881 ggatcacggg gggcaacctc tccaccgcgg ccgcttacat cgcggacgtg tccgcgccgc
     2941 aggagcgctc gaagaacttc gccatgatcg gcaccgcgtg gagcctcggc ctgatcctgg
     3001 ggccggccgc cggcggcctg atggggaccc tcgcgctgga ggcgcctgcc tacatcgccg
     3061 cggtcctctc cttcgtgaac gtggggctcg ggttcttcct tctgcaggaa tccctgcccc
     3121 gcgagcggag ggaaaccact cccctgcgcg gcgcggacct caacccgatc gtggccatcg
     3181 tcgacatggg ccgcaagccc ggcctgggct ggctcctgat cgtcatgagc ctgttcaact
     3241 tcgccttcaa cgggatcaac gccgtcggcg tcctgttcgt gatcgacaag ttccacgcgg
     3301 ccacctggca ggtcagcgtg atgctcatgc tcggcggcgg gaccatcgcc gtgtgcaact
     3361 ccctgctggt gccccgctgg gtgccgcggt tcggcgagag ggtcacggga gccgtcagcc
     3421 tgctgggcat ggcggtgttc gacgtggcca tcttcctggt gccgcttctg tggctggtca
     3481 tcctgatctc catggcggtg agcgccatga gctcgttcac cttcccgacc ttgaccacgc
     3541 tgtccaccga ccgggtgggc cacagcgagg tcggcctcct gctcggggta accaccgcca
     3601 tcgggagcct gatgaacatc ctcgggcccc tgtgggccgg cgccatctac gaccacgtca
     3661 tggtcggctc gccgtactgg atgggagcgg cggtgttcgt taccgccggc ctgatgctgc
     3721 tgagaccggc aacgatcgat cgcggcggcc gcaagcttta ccgccccgtc accgaacgat
     3781 agacttgggc catggcctcc cagaagatcc cgtctcccct cgcgtcgtgg tatccgtctc
     3841 cgctgcgctc ctggtaccgg gcgcccacgc gggtcgtgtg gcagagcgat gccggggtga
     3901 cccacgcctc ggagctgctg cggtccgggc ccgcccaggc caccctcgag ccgcccttcc
     3961 ctccctgcac gctgacccga gccgcggagg gcgccggcgg cctgctcctg gacttcggga
     4021 cggagctgca gggcttcgtg gagctgatca cgccgataag ccaggacaag aggccgggca
     4081 aggtgcgcgt tcgcttcggg gagtctgcct ccgaggcgat ggcggagctg ggggatccag
     4141 ggaatgcggg caacgaccac gccatgcggg accaggtggt cgagcttccg
//
LOCUS       DASRZS010000152         4022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3931624, whole genome shotgun sequence.
ACCESSION   DASRZS010000152 DASRZS010000000
VERSION     DASRZS010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3931624"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..535)
                     /gene="tpx"
                     /locus_tag="VFH83_03805"
     CDS             complement(32..535)
                     /gene="tpx"
                     /locus_tag="VFH83_03805"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET6485519.1"
                     /translation="MAQVLFKGSPMHTRGSLPAVGTKAPDFKLTTGDLRDVGLAEYKG
                     KRKILSVVHSLDTGTCAVSTRRFNTEATRLPDTVVLVISNDLPFAQKRFCEAEKIANV
                     VPLSQMRNRDFGKTYGMEITDGPIAGLLARSIIVIDESDKVVYTEQVQETTKEPDYEK
                     ALAAARK"
     gene            complement(613..1974)
                     /locus_tag="VFH83_03810"
     CDS             complement(613..1974)
                     /locus_tag="VFH83_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6485520.1"
                     /translation="MARLAIDGGSKAFRKRTGKLQPKIGIEEFLSIAERFGFSPAALK
                     RIRAAVRVEDLPPGGPHLGRYYGSPGPSKGEQLEALVRNTFGVKHVYAVSSGTGALHA
                     AFIAAGAGPGTEVICPGLGFMATAMAVELSGGVPIFCDVDESLQMDPERIEACITPRT
                     VALAPTHHWGNVADLDPILKVARKRKLKVVEDCAQAPGGKYRGRYVGTLGDIGCFSIS
                     SYKIIGAGESGLVVTGDGRLFDRLRQAAEGGGLWRPDRFAPPRYRGELFPGSNYRLSE
                     LEAAADLIQFHKIDEVCNRYRAVSRRIRGQLRSYAEITPQRINDPDGYIGFQLRFFPK
                     TPALGARITRALVAEGVAARTRGPRHKPDWHVARTMYPIVLKTGAIAGGSVFEDPRYL
                     QRGGSAEYRGICPVAADLFAREVAIDLDEWLSPQDCDAIAEGITKVLSAYCAEQPEGR
                     PWA"
     gene            2219..3076
                     /locus_tag="VFH83_03815"
     CDS             2219..3076
                     /locus_tag="VFH83_03815"
                     /inference="COORDINATES: protein motif:HMM:NF020927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HET6485521.1"
                     /translation="MAKRYTRTEFLARLRAQITQGRPLVMAGAGNGITARCMERGGVD
                     AIGVYTSSYLNAQGHGPLAAMLPIVDANELVYTMGQREVLRQVKEVPVVAGLAGVDPL
                     RDMRLFLEDCRRIGFSGVHNFPSVGWLDGEFRRNLEASDLGMEHEVSLLLMAREMDML
                     TVACAFNEADCERLMRDATPDIFVFHAGLGRAVSGNGRGTDLGEAAARSQRHYAIARR
                     IKPDIILLAQGAALANPEDVQYFLDHTDCHGVQLGCTIDPIAIERPVEERAAAYKAVH
                     FPTTGRPGI"
     gene            3132..>4022
                     /locus_tag="VFH83_03820"
     CDS             3132..>4022
                     /locus_tag="VFH83_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485522.1"
                     /translation="MSDAAGRLRGGFVAQLFALLMRASAPLTTVSILLVLASGVIPAA
                     ITVSTGAVVQAVQREVGGSSLREAARSALPTVLTLAGLFACQQLLGLAGYLATRSLGS
                     RLVLTLRETIMDATIDPPGIAHLEDPATAARIKLATGVEGRSFAPEEMVLGLSEMLST
                     RLRGIASAILLLGFHPWAPILLLASWSLIPFWVARQTRTQMARTESVTMDLRASDYSK
                     DLLTRPGPAKEVRIFGLAGWLLDRFSTQRRRGLEEIWRSRSRGRWTYVPYLALPAAAS
                     ALVFAVLASGAASGAIPLARF"
BASE COUNT          624 a         1365 c         1324 g          709 t
ORIGIN      
        1 ggcaaccgag cggggctgac cccggccctg gctacttccg cgcggccgcc agggccttct
       61 cgtagtcggg ctctttcgtg gtctcttgca cctgctcggt gtagactacc ttgtccgact
      121 cgtcgatcac gatgatcgat cgggccagaa ggcccgcgat gggtccgtcg gtgatctcca
      181 tgccgtaggt cttgccgaag tcccgattgc gcatctggga caggggcacg acgttggcga
      241 tcttctccgc ctcgcagaag cgcttctgcg cgaacggcag atcgttgctg atcaccagga
      301 cgaccgtatc gggaagccgg gtcgcctccg tgttgaaccg gcgcgtggac accgcgcagg
      361 tgcccgtgtc caggctgtgc accacactca ggattttccg cttgcccttg tattccgcca
      421 gaccgacgtc gcgcagatcc ccggtcgtca gcttgaaatc cggcgccttc gtgcctacgg
      481 cggggaggct gccccgcgta tgcatcggcg atcccttgaa cagaacctgt gccatatgcg
      541 ctccttggac caaccagtcc tgggctgaaa gtaagccctt ggtttccgcg gggcaaggaa
      601 gccgcgccgc cgtcaggccc agggacgtcc ctcgggttgt tcggcgcagt aggcggagag
      661 gaccttcgtg atcccttccg cgatggcatc gcagtcctgg gggctcagcc actcgtccag
      721 gtcgatggcg acctcgcggg caaacaggtc cgccgctacc gggcagatcc ctcggtactc
      781 ggcgctgccc ccgcgctgca ggtagcgtgg atcctcgaac acggacccgc cggcgatggc
      841 gcccgtcttg agaacgatcg ggtacatggt acgagcgacg tgccagtccg gcttgtgccg
      901 cgggccgcgg gtccgagcgg caaccccttc cgccacgagc gcccgagtga tccgggcacc
      961 gagggcggga gtcttcggga aaaaccgaag ctgaaacccg atgtacccgt cggggtcgtt
     1021 gatccgctgg ggcgtgatct ccgcgtagga acggagctgc ccgcggatgc gccggctcac
     1081 cgctcggtac cgattgcaga cttcgtcgat cttgtgaaac tggatcagat cggccgccgc
     1141 ctccagctcg ctcagacgat agttcgagcc ggggaagagc tcgccccggt agcgtggcgg
     1201 ggcaaagcga tccggccgcc acaggccgcc tccctccgcc gcctgtcgga gccggtcgaa
     1261 caggcgcccg tcccccgtga ccaccaggcc gctctctccc gctccgatga tcttgtagct
     1321 cgagatgctg aagcagccga tgtcgcccag cgttccgacg tatcgccccc gatacttccc
     1381 gccgggcgcc tgagcgcagt cctccacgac cttgagcttt cgcttgcgag cgaccttcag
     1441 gatgggatcg aggtccgcca cgttccccca gtggtgggtg ggagccaggg ccacggtccg
     1501 cggcgtgatg caggcctcga tgcgctcggg gtccatctgg agggactcgt ctacgtcaca
     1561 gaagatcggg acgccgccgg agagctccac ggccatggcg gtggccatga agccgagacc
     1621 gggacagatc acctccgtcc ccggtcctgc gccggcggcg atgaacgccg cgtgcagcgc
     1681 gcccgtgccc gagcttaccg catagacgtg cttcaccccg aaggtgttgc gcaccagcgc
     1741 ttccaactgc tcccccttgg agggtccggg agagccgtag taccgaccca ggtgaggtcc
     1801 cccggggggc aggtcctcca cgcgcaccgc ggcccggatc cgcttcagcg ccgccgggct
     1861 gaatccaaac cgctccgcga tggacaggaa ctcttcgatc ccgatcttgg gctggagttt
     1921 cccggtcctc tttcggaaag ccttggagcc accgtcgatc gccagtcgtg ccatgccggc
     1981 atcctaaccg atcccgggga tgctgggaaa tggccgaagc attcaccccg cggcgtgtcc
     2041 acccgcagca gctaccctct ggtgcgcgtc cgcggatccg aaacagcgcc ctctggtgcg
     2101 cgtccgcctt gaccgcgagg cggcatccca ctacgctgcc agggagccgg atcagaaagg
     2161 ggatgtaaaa tggtggatcc atcgtgtgcc ttctcgatcg atgccaagga gcgatgagat
     2221 ggcgaagcgc tacacccgga ccgagttcct ggctcgcctt cgcgcccaga tcacgcaagg
     2281 gcggcctctc gtcatggccg gcgcgggcaa cgggatcacg gcgcgctgca tggagcgcgg
     2341 cggcgtggac gccatcggcg tatacacgag cagttacctg aacgctcagg gccacggccc
     2401 tctggctgcc atgcttccca tcgtggacgc caacgagctg gtctacacca tgggtcagcg
     2461 ggaagtgctc cgccaggtca aggaagtacc ggtggtggcg ggtctggcgg gcgtggatcc
     2521 ccttcgcgac atgaggctgt tcctggagga ctgccgccgg atcgggttca gcggtgtgca
     2581 caacttccca tccgtcggct ggctggacgg agagttccgc agaaacctgg aggcctccga
     2641 tctgggcatg gagcacgagg tttccctgct gctcatggcc cgggaaatgg acatgctcac
     2701 cgtggcgtgc gcgttcaacg aagcggactg cgaacggctc atgagggatg ccaccccgga
     2761 catctttgtc ttccacgccg ggttgggccg ggccgtctcg gggaatggca ggggtacgga
     2821 tctgggggaa gccgccgccc gaagccagcg ccactatgcc attgctcgcc ggatcaagcc
     2881 ggacatcatc ctgctggcgc aaggtgccgc actggcgaac ccggaggatg tccagtactt
     2941 cctggatcat accgactgtc atggcgtgca gcttggctgc acgatcgatc ccatcgcgat
     3001 cgagcgaccc gtcgaggagc gggccgcggc ctacaaggcg gtccacttcc cgactaccgg
     3061 acgaccgggg atctagaagg caggacgccc ggtccattcc tcatgtgcgg tccgattgcc
     3121 tacggaggac tatgtccgac gcggcaggtc gcttgcgcgg ggggttcgta gcacagctct
     3181 tcgcgctcct catgcgggcc agcgctccgc tcaccacggt ttccatcctt ttggttttag
     3241 cctccggcgt catcccggcg gcaatcaccg tgtccacggg agcggtggtg caggccgtgc
     3301 agcgggaggt cggggggtcc agcctccggg aggcggcccg ctcagcgctt ccgaccgtgc
     3361 tcacgctggc agggctcttt gcctgccagc agctcctggg tcttgcgggc tacctggcaa
     3421 cgaggagcct cggctcccgg ctcgtgctta ctctgcgaga gacgatcatg gatgccacga
     3481 tcgatccgcc ggggatcgcc cacctggagg atccggccac ggccgcgcgg atcaagctgg
     3541 caacaggggt ggaagggcgg agcttcgcgc cggaggagat ggtcctcggg ctctcggaga
     3601 tgctctcgac ccggttacgc ggaatcgcct cggcgatcct gctcctgggg ttccatccct
     3661 gggctccgat cctgctcctg gcgtcctggt ccctgatccc attctgggtg gcgcgtcaga
     3721 cgcgcactca gatggcacgc acggagtccg ttaccatgga tctccgcgcc tctgactaca
     3781 gcaaggatct cctgacccga cccggtccgg ccaaagaggt gcgaatcttc ggcctggccg
     3841 gctggctgct ggaccggttc tccacccagc gccgccgagg cctggaggag atctggcgct
     3901 ccaggagccg cgggcggtgg acctacgtcc cctacctggc gctcccagcg gcggcctccg
     3961 cgctggtttt cgccgtcctc gcttccggcg cggcatccgg cgccattccc ttggcccggt
     4021 tc
//
LOCUS       DASRZS010000153         4175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4319356, whole genome shotgun sequence.
ACCESSION   DASRZS010000153 DASRZS010000000
VERSION     DASRZS010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4319356"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VFH83_03825"
     CDS             complement(<1..895)
                     /locus_tag="VFH83_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485523.1"
                     /translation="MRARGLLGELWRSAPGKLGLILLGILVVGSVVVVATYPPDFGPA
                     RWSNPALWADYPRSAPPVWVNLFSRERRMAHRLVQLSSPSEVTATNAGATAHYRMRFS
                     LAPGQVPTFLSFSVTDTQFQSQPPVITATLLRPDGDAVTLHREAVSGPRSGESPPYHR
                     NAEDPLRVVVDAEDDSLDALVTLFSDRYAAVVDRSAVEDQFGRMLFSKPVGGGFQTLT
                     GDYVLEVQIQGADPKDRAGSVRLVAGGSVFGALGTDALGRDLAQGLLFGLPIALLIGI
                     STAVVGTLIGMGLGLASGYFGG"
     gene            complement(898..1950)
                     /locus_tag="VFH83_03830"
     CDS             complement(898..1950)
                     /locus_tag="VFH83_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6485524.1"
                     /translation="MLALRTLVVRTLTMIGVLIVVQALVVVSLGATGYSDRMLSAAVG
                     EELRGLRTSLAQTIRDPDKLEQTLQARKIEIERSYGLDRPWYTRLPALVLRVFRLDLG
                     EAHTLRSFTGSSRVADIVRERIPNSMILLTTSLILTAIIGIAVGVRLAQRPGSFLDRF
                     ISSLSAVSFALPTWWLGILFILIFAFQLRVLPTGGMYSAPPPIGALPRFFDLLYHALL
                     PVITLVVASVGPYIYSVRTMTLNVAQADHVAVARAKGMPEATIARRHVLRVAAPPIVT
                     GLVLGFTGTLGGSILVETVFNWQGMGRLYYDAIAGTPDEMVIVALTFIYTLIYVAARL
                     LLEILYVVLDPRVRYE"
     gene            complement(1983..>4175)
                     /locus_tag="VFH83_03835"
     CDS             complement(1983..>4175)
                     /locus_tag="VFH83_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6485525.1"
                     /translation="LKTDAAEQLRGDPRFTLYEAPASTVSLLLNPAPAPQGSLNPFSL
                     VDVRRAMQYLVDRQFMAHDIYRGHATPMVSQVSPTDFDYLTVYDIERSSGITYDPEYG
                     RQMIAKAMQQAGAQMSGGLWSYGGQPVRIKIVGRVEDERRNIADLVRVALEKAGFSVA
                     VTYLPFAAAVDLTYSSDPQTLQWNVYTEGWGRSAPSRYDYANVNSMTAPWMGNMPGWQ
                     QTGFWQYQQAELDTLGKRLYRGEFKTLEERNTIYRRMTELGLQESVRVWLVTVTNSFP
                     ARKELVRASRDLVAGVQAPWTLREAYVPGKTDLTVGNLWVWTERTTWNPIGGFGDVYS
                     SGIWKYLSDPPMWNHPFTGAPIPVRAAYTVQTAGPNGKLAVPPDAVVWNTTAHRWDPV
                     AAGARATSRVVFDYAKYFQSKWHDGSPITMADVIYSIAQGFDMAYDPDKARIETALAV
                     TARPFLDTYRGFRILDDHRLEVYVDYWHFQDSFIASYASPSGLAMPWEILAAMDDLVF
                     NQRRAAYSDTAAARFNVPWLSLVMDRDVGLVRRTLRSFLQNATLPAAYLTVGGKLLTS
                     DADASARYTAALKWADTTGNLVIGNGPFFLARYDPPAQYAELHAFRDPGYPFKPGDWS
                     FGEPPTLSIGPVPALTVTAGQATQVTVTVKGSGTLGLRYLLVDSAKRTVVTAGEASAG
                     SAPGSFVVALPATATAKLSAGLNELALVAYSDAIAQVANRGVDVNVTR"
BASE COUNT          707 a         1416 c         1416 g          636 t
ORIGIN      
        1 tgccgccgaa gtagccgctg gccagcccca gtcccatccc gatcagggtg cccacaacgg
       61 ccgtcgagat tccgatgagg agcgcgatcg gcagcccgaa gaggagcccc tgagcgaggt
      121 ctcgacccag ggcgtcggtg cccaacgccc cgaataccga gccccccgct accagacgca
      181 cggagcccgc gcggtccttc gggtccgcac cctggatctg gacttccagg acatagtccc
      241 ccgtcagggt ctggaatccc cctcctaccg gtttggaaaa gagcatccgg ccgaactggt
      301 cctccacggc cgagcgatcg accaccgctg catagcggtc ggagaagaga gtgaccagcg
      361 catccaggga gtcgtcctcg gcgtcgacca ccacccgcag gggatcttcg gcgttgcggt
      421 ggtacggcgg gctctcgccc gaccgcggcc cgctcacggc ctcccgatgc agcgttacgg
      481 cgtcgccatc cgggcgcagg agcgttgccg tgatcaccgg aggctgggac tggaactggg
      541 tgtcggtgac ggagaacgac aggaacgtcg gcacctggcc gggcgccagg gaaaaccgca
      601 tccggtagtg tgccgtggct cccgcgttgg tggcggttac ctcactggga gaggagagct
      661 gaaccaaccg gtgcgccatg cgcctttctc gcgagaagag gttcacccag accggcgggg
      721 cggaacgagg atagtcggcc cagagcgccg ggttgctcca ccgcgccggt ccgaagtccg
      781 gcgggtaggt ggcaacgacc acgaccgacc ctaccaccag gatcccgagc aggatgagcc
      841 ccagcttccc gggtgcgctc ctccagagct ctccgagcag accgcgggcg cgcatcccta
      901 ctcgtacctc acgcgggggt ccagcaccac gtagaggatc tccagcagaa gccgcgcggc
      961 cacgtagatc agggtgtaga tgaaggtcag ggccacgatc accatctcat ccggcgtgcc
     1021 ggcaatcgcg tcatagtaga ggcggcccat cccctgccag ttgaagaccg tctccaccag
     1081 gatcgatccg cccagcgtgc cggtgaaacc cagcaccagg ccggtgacga tgggaggagc
     1141 cgcgacgcgc aggacgtgtc ggcgcgcgat cgtggcttcc ggcatcccct tggcgcgggc
     1201 cacggcaacg tggtccgcct gggccacgtt cagcgtcatg gtgcgaaccg agtagatgta
     1261 cggaccgacg ctcgccacca ccagcgtgat caccggcagg agggcgtggt agagcaggtc
     1321 gaagaaccgc gggagggcgc cgatgggcgg cggagcgctg tacatgccgc ccgtgggcag
     1381 gacccgaagc tggaaggcga agatgaggat gaagaggatc cccagccacc acgtgggcag
     1441 ggcgaaggac accgccgaca ggctcgagat gaaccggtcg aggaacgagc cgggccgctg
     1501 ggccaggcgc acccctacgg cgatcccaat gatggccgtc aggatcaggg atgtggtaag
     1561 gaggatcatc gagttgggga tccgctcccg cacgatgtcc gctacccggc tggatcccgt
     1621 gaagctgcgc agcgtatgcg cctcccccag gtccagacgg aacacccgca gcaccagggc
     1681 cggcagccgt gtgtaccacg gccgatccag accgtaggag cgctcgatct cgatcttgcg
     1741 ggcctggagc gtctgctcca gcttgtccgg gtcccggatg gtctgagcca gcgacgtgcg
     1801 caggccgcga agctcctccc ccacggccgc gctcaacatg cggtccgagt agccggtggc
     1861 gccgaggctc accacgacaa gggcctgcac cacgatcagc acgccgatca tggtcagggt
     1921 gcgcaccacg agggtccgaa gcgccagcac gtttcccctc cgagagccgc tgggcgccgg
     1981 ctctatcgtg tcacgttcac gtccacaccg cggttcgcca cctgggcgat ggcatcgctg
     2041 tacgccacca gcgccagctc gttcaggccc gcgctgagct tcgcggtggc agtggccggc
     2101 agcgccacca cgaaggagcc cggcgcgctg ccggcgctcg cctcccctgc ggtcacgacc
     2161 gtgcgcttcg cggagtccac cagcaggtag cgcagcccca gcgtgccgct gcccttcacg
     2221 gtcaccgtga cctgggtcgc ctggccggcg gtgacggtga gcgccggtac gggtccgatg
     2281 gaaagggtcg gaggctcccc gaagctccag tcccccggct tgaacggata gcccgggtcc
     2341 cgaaaggcgt gaagctccgc gtattgcgcc ggcggatcgt agcgggccag gaagaacggg
     2401 ccgttcccga tcaccaggtt ccccgtggta tccgcccact tcagggcggc ggtgtagcgc
     2461 gcggaggcat ccgcgtccga ggtcaggagc ttgcccccca ccgtcaggta ggcggcaggg
     2521 agggtcgcgt tctggaggaa cgaccgaagg gtccgtcgga ccagccccac gtcccggtcc
     2581 attaccaggc tcagccatgg gacgttgaac cgggccgcgg ccgtgtcgct gtacgcggcg
     2641 cgcctctggt tgaagaccag gtcatccatc gccgccagga tctcccatgg catggccaat
     2701 cccgacgggc tggcgtagga cgcgatgaag ctgtcctgga agtgccagta gtcgacgtag
     2761 acctccagcc ggtgatcgtc caggatgcgg aatccccgat aggtgtccag gaacgggcgg
     2821 gcggtgaccg ccagggcggt ttcgattctg gccttgtccg ggtcataggc catgtcgaag
     2881 ccctgggcga tggagtagat cacgtccgcc atggtgatgg gcgagccatc gtgccacttg
     2941 gactggaagt acttcgcgta gtcgaagacc accctgctgg tggccctggc gcccgccgcc
     3001 accgggtccc aacggtgggc ggtcgtgttc cagaccacgg catccggggg gaccgccagc
     3061 ttcccgttcg ggccggccgt ctgcacggtg tacgcggcgc ggaccgggat gggcgccccc
     3121 gtgaacgggt ggttccacat cggtggatcg ctcaggtact tccagatccc gctgctgtag
     3181 acatcaccga acccaccgat ggggttccag gtggtccgct ccgtccagac ccacaggttg
     3241 ccgacggtca gatccgtctt gccagggacg tacgcctccc ggagcgtcca gggcgcctgc
     3301 actccggcca ccaggtcacg ggacgcgcgc acgagctcct tgcgcgcggg gaagctgttc
     3361 gttacggtca ccagccagac gcggacgctc tcctgcaagc ccagctccgt catccggcgg
     3421 tagatggtgt tccgctcctc cagggtcttg aactccccgc ggtacagccg cttgcccagc
     3481 gtgtccagct ccgcctgctg gtattgccag aagcccgtct gctgccagcc cggcatgttg
     3541 cccatccagg gcgcggtcat ggagttcacg ttggcgtaat cgtatcggga tggcgcggac
     3601 cttccccatc cctccgtgta gacgttccac tgcagggtct gcggatcgct ggagtacgtc
     3661 aggtccaccg cggccgcgaa ggggaggtag gtgaccgcca ccgagaaccc cgccttctcc
     3721 agcgccacgc gcaccaggtc cgcgatgttc cgccgctcgt cctccacgcg tccgacgatc
     3781 ttgatccgca ccggctgccc gccatagctc cagagacccc cgctcatctg cgccccggcc
     3841 tgctgcatgg ccttggcgat catctggcgg ccgtactccg ggtcatacgt gatgccggag
     3901 gaccgctcga tgtcgtagac ggtgaggtag tcgaagtcgg tcgggctcac ctgggagacc
     3961 atgggggtgg catggccccg gtagatgtcg tgggccatga actgacgatc caccaggtac
     4021 tgcatggcgc gccgaacgtc gaccagggag aagggattga gcgagccctg cggcgcgggt
     4081 gccgggttca agagcaggga gaccgtcgaa gccggagcct catacagggt gaaccgtgga
     4141 tcgccgcgaa gctgctcggc cgcgtcggtc ttgag
//
LOCUS       DASRZS010000154         4602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3932333, whole genome shotgun sequence.
ACCESSION   DASRZS010000154 DASRZS010000000
VERSION     DASRZS010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3932333"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1711
                     /gene="ftsH"
                     /locus_tag="VFH83_03840"
     CDS             <1..1711
                     /gene="ftsH"
                     /locus_tag="VFH83_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET6485526.1"
                     /translation="KIASGEIKQVQMDDSSYLGLTVTKAQETSSSQQQNQAVTVYKTV
                     RVEDPQFIALLDSNHVEYYAVEKREHPFLNLLLSTVLPIVVMVVIWRLAFQRMGNPGS
                     GVLSFGQNKAKVVAEGDIKVRFADVAGVDEAKEELVEVVDFLKRPERYTAIGGKIPKG
                     VLLVGSPGTGKTLLAKAVAGEAGVPFFRISGSDFVEMFVGVGAARVRDLFKQAREKAP
                     CIVFVDELDAIGKSRLNAMSSNDEREQTLNQLLVEMDGFDSTSGVVILAATNRPEILD
                     PALLRPGRFDRTVLVDHPDLAGREEILKIHTRAVKLDSSVDLKVVARMTPGLVGADLA
                     NIVNEAALLAVRSKRERISQADFEEAIEKNIAGLRKKNRLINPKEREVVAYHEVGHAL
                     MAVHTPGADPVQKVSIVPRGFGALGYTLQTPTEERFLLSYDELIGRVDVLLGGRAAEQ
                     VVYGKVSTGAANDLVKATDIVKRMITEYGMSEKFKNVVLSQQRAPLLGAQAEPGVIRE
                     YAESTQTYVDEEIARIVNQRYEAVLGVLGGDKSSLERIARKLLEVETLDEKAFQGILN
                     GAS"
     gene            1738..2202
                     /locus_tag="VFH83_03845"
     CDS             1738..2202
                     /locus_tag="VFH83_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6485527.1"
                     /translation="MYGKILVPLDGSERAEKILPYVEDIARQFKSEVLLLLVIEAVPE
                     PLGPGRASLPAQPDEHDPVLRSSRAYLMGIHDSLSRIGVASSIHVLSGNVVASIVELA
                     SREEVYMVAMASHGRSGLARVYYGSVADGVLHQIDRPLLLVRSVSPPREGAG"
     gene            2223..4490
                     /locus_tag="VFH83_03850"
     CDS             2223..4490
                     /locus_tag="VFH83_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6485528.1"
                     /translation="MSSNQERVGMLIGKLGTWYEDKAWLEKGRMTKRLWPYDSMFSPI
                     RVNRLTIKNRLVMAPMGNINMAEETGRPSAKMVQYYTERAKGGVGLITSGLIPVSHGI
                     DPSVTERGDLSYFPRFDRSRTVYSGWRDIAERCHAHGARFFIQLTPGLGRVGTPECLI
                     NKRRFPVSASWNPNFYIPAVPCRPLTDTQARRIIRRAGQAAADARAAAIDGVYLHGHE
                     GYLLEQMTNRAFNRRKLGAFTDTKAFGVQIVREIRRRTSPDYPIMYRIDLSLVLNETY
                     AERMTSVRGLRKFRNERTVVETLEFMEALVKAGVDLFDVDLGCYDNWWLPHPPTPMAP
                     GCYLEVSRIVKEHFAKKGVRSNAGLAVPVVAVGKLGYPDLAEAALRNGDCDMIMLGRP
                     LLADPEWPNKAFAGRVGSICPCIGDQEGCINEFVEGGHPQCTVNPRTGFEDILSSDLM
                     PASKPKRVAVVGGGPAGMVTACLAARRGHTVTLYEARSELGGMLVPGSKPAIKNDIVN
                     YLDYLRTEVARASEECGLKVQLLKAPTPGELRSAGYDSVVTATGSRQTRPPVPGIDSR
                     NVVYAVDLLRDPELVKGATSVVVVGGGVVGCETAYFLAHELGKKVAVIEMLPLIMKGV
                     CTANRGWLIHFLEERGVPLYNCTKLASVAAGEVTVIRNVSRTVPDPMITWSPLLPENI
                     QNPFATKLKLEEREQRLPADLVVLAAGARPDDSPYYAFVRAHAAAEIRNIGDSDSVGR
                     VLEATRAAYATATAL"
BASE COUNT          864 a         1362 c         1600 g          776 t
ORIGIN      
        1 gaagatcgcc agcggggaga tcaagcaggt gcagatggac gacagctcct acctcggtct
       61 gacggtgacc aaggcgcagg agacatccag ctcgcagcaa cagaaccagg cggtcacggt
      121 gtacaagacc gtccgcgtgg aggacccgca gttcatcgcc cttttggaca gcaaccacgt
      181 ggagtactac gcggtggaga agcgcgagca cccgtttctg aacctgctgc tcagcacggt
      241 gctgcccatc gtggtcatgg tggtgatctg gcggctggcg ttccagcgga tggggaaccc
      301 gggctctggg gtgctgagct tcgggcagaa caaggcgaag gtggtggcgg agggggatat
      361 caaggtccgc ttcgcggatg tggcaggcgt ggatgaggcc aaggaagagc ttgtggaggt
      421 ggtggatttc ctgaagcggc ccgagcgcta cacggccatc gggggcaaga ttcccaaggg
      481 ggtgctgctg gtgggatccc ccgggacggg caagaccctg ttggccaagg cggtggcggg
      541 ggaggccggg gtgccgttct tccgcatcag cggctcggac tttgtggaga tgttcgtggg
      601 ggtgggggcg gctcgggtgc gggacctgtt caagcaggcg cgggagaagg ctccctgcat
      661 cgtgttcgtg gatgagctgg atgccatcgg caagagtcgg ctgaacgcga tgagctccaa
      721 cgacgagcgg gagcagacgc tgaaccagct tctggtggag atggacggtt tcgactcgac
      781 cagcggggtg gtgattctgg cggcgaccaa ccgacccgag attctggacc cggcgcttct
      841 gaggccgggg cgcttcgacc gcacggtcct ggtggaccat ccggaccttg cgggtcggga
      901 ggagatcctg aagatccaca cgcgagcggt gaagctggac tcctcggtgg acctgaaggt
      961 ggtggcgcgc atgaccccgg ggctggtggg cgcggatctg gccaacatcg tcaacgaagc
     1021 ggcgttgctg gcggtgcgct ccaagcggga gcggatcagt caggcggact tcgaggaagc
     1081 gatcgagaag aacattgccg ggctgcgcaa gaagaatcgg ctgatcaacc cgaaggaacg
     1141 ggaagtggtg gcctaccacg aggtgggaca cgcgctgatg gcggtgcaca ccccgggggc
     1201 cgatccggtg cagaaggtat ccattgtgcc gcgagggttc ggggcgctgg gctacacgct
     1261 gcagacgccg accgaggagc ggtttctgct gagctacgac gagctgatcg gcagggtgga
     1321 cgtgcttctg ggagggaggg cggcggagca ggtggtttac gggaaggtgt ccacgggggc
     1381 ggccaacgat ctggtgaagg cgacggacat cgtgaagcgg atgatcaccg agtacgggat
     1441 gagcgagaag ttcaagaacg tggtgctcag tcagcagcgg gcgccgctgc tgggggcgca
     1501 ggcggagccg ggggtgatcc gagagtacgc ggagagcacg cagacgtacg tggatgagga
     1561 gatcgcgcgg atcgtcaacc agcgctacga ggcggtgctg ggggtgttgg gaggggacaa
     1621 gagcagtctg gagaggattg ccaggaagct gctggaagtg gagacgctgg atgagaaggc
     1681 gttccagggg atcctgaacg gcgcttcctg agcgtccgcg caaggggaga agaaaccatg
     1741 tacgggaaga tcctggttcc tctggacgga tcggagcgcg cggagaagat actaccctat
     1801 gtcgaggaca ttgcgcggca gttcaagtca gaggtgcttc tcctgctagt catcgaggcg
     1861 gtgccggaac ctcttggccc tgggcgggca agtcttccgg cacaaccgga tgagcacgat
     1921 ccggtccttc ggagctcccg cgcctacctc atggggattc acgactcact ctccagaata
     1981 ggggtagcct cgagcatcca tgtgctcagc ggaaatgtgg tcgcctccat tgtcgagctc
     2041 gcttctcgag aagaggtcta catggtcgcc atggcaagcc acggaagaag cggactggcc
     2101 agagtatact acggtagtgt cgcggacggg gtgctgcatc agatcgaccg tccgcttctg
     2161 ctcgttcgat ccgtgtcacc accgcgggag ggcgccggct gacccccaga gaaggagccg
     2221 atatgtcgag caatcaggaa agagttggga tgctgatcgg gaagctcggg acgtggtacg
     2281 aggacaaggc ctggctcgag aagggccgca tgacaaagag gctctggccc tacgattcga
     2341 tgttctcgcc gatccgcgtg aaccgcctca ccatcaagaa ccgcctcgtc atggcgccga
     2401 tgggaaacat caacatggcc gaggagacgg gaagaccgag cgccaagatg gtccagtact
     2461 acaccgagcg agccaagggc ggcgtgggtc tcatcacctc cggcctcatc ccggtgagcc
     2521 acggaatcga cccttcggtt acggagcgcg gcgacctctc ttacttccct cgctttgacc
     2581 gctcgcgcac ggtctactcg ggctggcgcg acattgccga gcgctgccac gcccatgggg
     2641 cgcgcttctt cattcagctc acgcccggac tcggccgggt gggcaccccc gagtgcttga
     2701 tcaacaagag gcggttcccc gtctccgcgt cgtggaaccc gaacttctac attcccgccg
     2761 ttccctgccg gcctctcacg gacacccagg cgcggcgcat cataaggaga gctggccagg
     2821 ccgcggcgga cgccagggcc gcggcgatcg acggcgtcta tctgcacggt cacgaggggt
     2881 acctcctcga gcagatgacc aaccgggcgt tcaatcgacg caagcttggc gccttcaccg
     2941 acacgaaggc cttcggcgtg cagatcgtaa gggagatccg gcggcgcacc agccccgact
     3001 atccgatcat gtaccgcatc gacctctccc tcgtcctcaa cgagacctac gctgagcgca
     3061 tgacgtccgt caggggcctg aggaagtttc gcaacgaacg cacagtcgtc gagactctcg
     3121 aattcatgga ggccctcgtg aaggcgggtg tcgacctgtt cgacgtcgac ctgggatgct
     3181 acgacaactg gtggctgccc cacccgccga cacccatggc acccggctgt tacctggagg
     3241 tgtcgcgcat cgtcaaggag cacttcgcga agaagggcgt caggtccaac gccggcctgg
     3301 cggtgcccgt ggtcgccgtt ggcaagctcg gctacccgga cctcgccgaa gcggccctgc
     3361 gaaacgggga ctgcgacatg atcatgctcg gccgtcccct cctggccgat cctgagtggc
     3421 ccaacaaggc cttcgcgggc agggtgggaa gcatctgccc ctgcatcggc gaccaggaag
     3481 gctgcatcaa cgagttcgtg gagggtggac accctcagtg cacggtgaac cctcggacgg
     3541 gcttcgagga catcctctcc tcggacctca tgccagcatc gaagcccaag cgggtcgccg
     3601 tggttggcgg agggcccgcc ggcatggtga cggcctgtct ggccgcacgt cgcgggcaca
     3661 ccgttaccct ctacgaggca cgatccgagc ttggcggaat gctcgtcccc ggatcgaagc
     3721 cggccatcaa gaacgacatc gtgaactacc tcgactacct gaggaccgag gtcgccaggg
     3781 cttcggagga gtgcggcctc aaggtccagc tcctgaaggc cccgacgccc ggggagttgc
     3841 gttccgcggg ctacgacagc gtcgtgacgg ctacgggctc ccgacagacc agaccgcccg
     3901 tcccgggcat cgattccagg aatgtcgtct atgcggtaga cctcctgcgc gaccccgagc
     3961 ttgtgaaggg cgcgaccagc gtcgtggttg tcggcggcgg cgtggtcggc tgcgagacgg
     4021 cgtatttcct ggcccacgag cttggaaaga aggtcgcggt tatcgagatg cttcccctga
     4081 tcatgaaggg ggtgtgcacg gcgaaccggg gatggctcat ccattttctt gaagagagag
     4141 gcgtcccgct ctacaattgc acgaagctcg cctccgtcgc ggccggcgag gtgaccgtga
     4201 ttcgcaacgt gtcccggacc gttcccgacc ccatgatcac gtggtccccc ctcctcccgg
     4261 agaatatcca gaatcctttc gcgacgaagc tcaagctcga ggagagggag cagcggctcc
     4321 ccgccgacct ggtcgttttg gccgcgggcg ctcgtcccga cgattctccc tattacgcgt
     4381 tcgtgagggc gcacgccgcg gcggagatca ggaacatcgg cgactctgat tccgtcggcc
     4441 gcgttctcga ggccaccagg gccgcctacg cgacggcaac ggcactgtga gcgctgggat
     4501 ggaacggttg atgttcgcac gctcgccgca ggggcgcttc tgcgccgata cttcatcgcc
     4561 tgatctcacg aaggagaccc tccttggacc cttcgataga ag
//
LOCUS       DASRZS010000155         3571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1621133, whole genome shotgun sequence.
ACCESSION   DASRZS010000155 DASRZS010000000
VERSION     DASRZS010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621133"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VFH83_03855"
     CDS             complement(<1..603)
                     /locus_tag="VFH83_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6485529.1"
                     /translation="MNLHSRDLGGDGPAVLILHGLFGSSQNWVGMGRRLAAAGRIFAL
                     DLRNHGDSPHAPSMTLDDCVEDLRESLPAAPAVVIGHSLGGLVAMGFAIAHPEQVAGV
                     CTIDIAPRPYPGDHEREYACLETDISGCATRAELDALLAPLLPEERERSFLLMNAVRD
                     GSGFRWRLNVPALKSHTVSRDLGPESGSFAGESLLVACGRS"
     gene            704..1990
                     /locus_tag="VFH83_03860"
     CDS             704..1990
                     /locus_tag="VFH83_03860"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET6485530.1"
                     /translation="MPETARLTLEGKDYDLPVVRGSEGEKAIDVSALRKATGFITLDK
                     GYENTGSCRSAITFIDGEKGILRYRGYPIEDLAEKSTFTEVAYLLLYGELPTTTQLEE
                     FSTFLNESSLIHEDMLHFFVGFPRGSHPMGILATMVASLSTFYPVPEIMDEKAERQIL
                     ANLISQVRTIAAISYKESIGEPIVYPSYRLRYVPNFLTMMFSSPVKETRLDDDVVRAI
                     DLFLILHADHEQNCSTSTVRMTGSSLANVYAVISAGISALWGPRHGGANQEVIRMLER
                     IRDEHGDGTEYVRKAKDRNTPDRLMGFGHRVYKSFDPRAKILKDACHKLLRKPGMNDP
                     LFDIALRLEEIALKDEYFISRNLYPNVDFYSGLILRATGIPYDMFTVLFAIGRTPGWL
                     AHWREMYHGEDFRIMRPRQIYTGATCRHYVDRSARG"
     gene            2014..2874
                     /locus_tag="VFH83_03865"
     CDS             2014..2874
                     /locus_tag="VFH83_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6485531.1"
                     /translation="MLVMAASSVLFSVVGVFVPLAAPANSSIIAAARFVVGIVSIVGF
                     SFIGLGRLTTVNRWWLVLRGVIGGLSVYLYYRGIAKLGLGTGTILNYSYPVFAALLAP
                     LLVKERLTWDVVAAVIVSFGGIWLVVSPSVLQGGNAAGGLPLGLETAFALLGGILAGV
                     AVVAIKKLRETDSPQIIYLAQCFFGLLIVGWPAATSSFSFGAVTWVILLGIGGLATVA
                     QLMMTWAYKHVPATEGSLLGFLVPVISVGLGAAVFGERMRAASLAGSAIVLLCCGYVA
                     FRERIWRLVG"
     gene            complement(2875..>3571)
                     /locus_tag="VFH83_03870"
     CDS             complement(2875..>3571)
                     /locus_tag="VFH83_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET6485532.1"
                     /translation="ASNAYAICIQAGAIIAVFVLYFGRIKGMVLGIFGRDRDGLKLLG
                     NLVVALIPAVLFGVLLEDRIKEYLYGVWPVAGAWLVGGVFILAVLARRKSNEGKALES
                     LGTGSALVIGLVQVLALWPGVSRSLATITAGLFVGLSVSAAVEFSFLLGLLTLGGATI
                     YEALKVGPLIVSTFGWLSPVIGLVVAAVAAFIAVRWMVEYLRTRSLAIFGWYRIGIAI
                     VAGVLVVTGAIAG"
BASE COUNT          628 a         1223 c         1122 g          598 t
ORIGIN      
        1 tgagcggccg cacgccacca ggagggactc ccccgcgaag gaacccgact ccggacccag
       61 gtcgcggctg accgtgtggc ttttgagcgc agggacgttg agacgccatc ggaagcccga
      121 gccgtctctg accgcgttca tgaggaggaa cgaccgttcc cgttcctccg gcaagagggg
      181 agccagcagc gcgtccaact ccgcccgggt ggcgcagccg ctgatgtccg tctccagaca
      241 cgcgtactcc cgttcgtggt cgccggggta gggccgcggc gcgatgtcaa tggtgcacac
      301 tccggccacc tgctcgggat gagcgatggc gaaccccatg gccaccagcc cgcccaggga
      361 gtgaccgatg accacggccg gagccgccgg gagagactct ctcaagtctt cgacgcagtc
      421 atccagggtc atcgacggcg cgtggggaga gtcgccgtgg ttgcgcaggt ccagcgcgaa
      481 gattcgacca gcggcggcca gcctgcgccc catcccgacc cagttctgcg aggagccgaa
      541 caacccgtga aggatgagga ccgccgggcc gtcgcctccg aggtcgcggc tgtgaaggtt
      601 catggaagga gagtagccgg cgccgtgcct cgcggcaacg cggaccggca atgttgactt
      661 tcatcgacga ttgccgtctc atagcgcatc aaaggaggaa cccatgcccg agaccgcccg
      721 gctaaccctg gagggcaaag actacgattt gcccgtcgtg cgcggctccg agggtgagaa
      781 ggccatcgac gtcagcgccc tgcgcaaggc caccggtttc atcaccctgg ataagggcta
      841 cgagaacacg ggttcctgcc gttccgccat tacgttcatc gacggggaga aggggatcct
      901 ccgctaccgc gggtatccca tcgaggatct ggcggaaaaa tcgacgttca cggaggtcgc
      961 ctacctcctc ctgtacggag agctcccaac cacgacgcag ctcgaagagt tctccacgtt
     1021 cctgaacgag tcctccctca tccacgagga catgcttcac ttcttcgtgg ggttcccccg
     1081 cggatcgcac cccatgggga tcctggccac catggtggct tccctctcca cgttctatcc
     1141 ggttcccgag atcatggacg agaaggcgga gcgccagatc ctggccaacc tgatctccca
     1201 ggtgcgcacc atcgccgcca tctcctacaa ggagtccatc ggagagccca tcgtctaccc
     1261 ctcgtaccgg ctgcgctacg tgcccaactt cctgaccatg atgttctcct ccccggtcaa
     1321 ggagacccgt ctggatgacg acgtggtgcg cgccatcgac ctcttcctga tcctccacgc
     1381 ggaccacgag cagaactgca gcacctcgac ggtccgcatg acgggctcca gcctggccaa
     1441 cgtctacgcc gtcatctccg cggggatctc cgcgctctgg ggcccgcgcc acggcggggc
     1501 caaccaggag gtcatccgga tgctggagcg catccgggac gagcacggcg acggcaccga
     1561 atacgtgcgc aaggccaagg accgaaatac tccggaccga ctcatggggt tcggccaccg
     1621 ggtctacaag agcttcgatc ctcgcgccaa gatcctgaag gacgcctgcc acaagctcct
     1681 tcgcaaaccg gggatgaacg acccgctctt cgacatcgcc ctccgtctgg aagagatcgc
     1741 gctgaaggac gagtacttca tctcccgcaa cctctatccc aacgtggact tctactccgg
     1801 tctcatcctc cgcgccacgg gaatccccta cgacatgttc acggtgctct tcgccatcgg
     1861 ccgcacgccg ggctggctgg cgcactggcg cgagatgtac cacggagaag acttccgcat
     1921 catgcggccg cggcagatct acaccggagc cacctgccgc cactacgtgg accgatcggc
     1981 gcgcgggtag tgcttgccac ccaggcgacc gggatgctgg tcatggcggc ctccagcgtc
     2041 ctgttctccg tggtcggggt ctttgtgcct ttggccgcgc ccgccaacag ctcgatcatc
     2101 gccgcggcgc ggttcgtcgt ggggatcgtg tccatcgtag ggttctcctt catcgggctg
     2161 ggccggctca ccacggtgaa ccgctggtgg ctggtgctga ggggcgtcat cggcgggctt
     2221 tccgtatacc tgtactatag agggatcgcg aagctcggtt tagggacagg caccatcctc
     2281 aactacagct atcccgtctt tgcggccctg ctggcgcccc ttctcgtaaa ggagcgcctt
     2341 acttgggacg tggtcgcggc ggtgatcgtc tccttcggcg gtatctggct ggtggtcagc
     2401 cccagcgtcc tccagggcgg aaacgcagcc ggggggcttc ccctcggact ggaaacggct
     2461 ttcgcgctcc tgggcggcat cctggcgggc gtcgcggtgg tggccatcaa gaagctgcgg
     2521 gagaccgact caccccagat catctacctg gcccagtgct tcttcgggct cctcatcgtg
     2581 gggtggccgg cggccaccag ctcgttctcg ttcggggccg tgacctgggt catcctgctg
     2641 gggatcggcg gcctggccac cgtggcgcag ctcatgatga cctgggccta caagcacgtg
     2701 ccggcaacgg agggaagcct cctgggcttc ctggtgccgg tgatcagtgt agggctggga
     2761 gccgcggtct tcggagagcg gatgcgcgct gcatccctgg cggggtcggc gatcgtcctt
     2821 ctctgctgcg gctacgtggc gtttcgagag aggatctgga gactggtcgg gtgactaccc
     2881 ggcgatagcc cccgtgacga ccagcacgcc cgccacgatg gcgatgccga tccgatacca
     2941 gccgaagatc gccaggctcc gcgtgcgcag gtactccacc atccagcgga ccgcgatgaa
     3001 cgccgccacg gcggccacca ccagaccgat caccggggag agccagccga aggtgctgac
     3061 gatgaggggc ccgaccttga gcgcctcgta gatcgtggcg ccgccgagcg tgagcaaccc
     3121 gagcaggaag ctgaactcca cggcggccga taccgaaagc ccgacgaaaa ggccggccgt
     3181 gatcgttgcc aggctacggc tcaccccggg ccacagcgcc agcacctgta ccagaccgat
     3241 cacgagcgcg ctgccggttc ccaggctctc cagcgccttg ccttcgttgg acttgcggcg
     3301 cgccaggacc gccaggatga aaacgccgcc gaccagccac gctccggcca ccggccatac
     3361 gccgtagagg tactccttga tccggtcctc caagagcacc ccgaacagga ccgccggaat
     3421 cagcgccacc accaggttgc cgagcagctt cagtccatcc cggtcccggc cgaagattcc
     3481 gaggaccatc cctttgatcc tgccgaagta cagcacgaac acggcgatga tggcgccggc
     3541 ctggatgcag atggcatagg cgttggacgc g
//
LOCUS       DASRZS010000156         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5474792, whole genome shotgun sequence.
ACCESSION   DASRZS010000156 DASRZS010000000
VERSION     DASRZS010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5474792"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VFH83_03875"
     CDS             complement(<1..849)
                     /locus_tag="VFH83_03875"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HET6485533.1"
                     /translation="MTTTGTRVREDSDLGRIPGLVIFYIGCAVHLLFLVSLLTHWLDP
                     LFFEATTDYGQASDYFGIYQAGDNLTQGRSIYLEGGRPEDSDRHVPYYYFYRYLPPTA
                     TFMALTALLLKPWPSYWVWVVINEALLGLTLFSLRRLRSPPSALRRALAGLTLGFSPL
                     YLELWMGQFSLLMAVLLWVTFWPELRAGAEGGGRIPAAGEGEGPPESWPQAGPKERFR
                     ALLRQPSLWCWSASVALKNYPAFFAFPWLRLGRWRRVFLGAGLVALSCLPYFLLRPHD
                     VGLYRTA"
     gene            1345..>2746
                     /locus_tag="VFH83_03880"
     CDS             1345..>2746
                     /locus_tag="VFH83_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6485534.1"
                     /translation="MASGRRPDIRSSEDTTRKALRESDRNFQTFFDSMGEMALVSRPE
                     GRILRTNPELRLRLGFTERELRRMSLADLQPPDQRADAAVLLECLIRGDVESAPLPLQ
                     TRHGAVMGADARAWRGRWNSQPCFFVLFRDLSLEQEAQQRFERLFRSNPALMAVSSFP
                     DGCFLDVNEAFLQRTGYRRDELIGHTSQELGLFLRPDQHATMVERLRRQGRLVNAEMQ
                     IRCRNGEIRDGLFSGELVQSQGRIHFLTVMADITDLKRADHELRGTVGRLEEATARAR
                     ELAETAAQASLAKSEFLANMSHEIRTPMNGVIGMTGLLLGTSLDAEQRRYAETIRRSG
                     ESLLALVNDILDYSKMEARKLDLESLDFDLSVLLDDFADSLALRAHERGLELVVCPQP
                     GVSVLLRGDPGRLRQVLTNLVGNSLKFTERGEVVVTVGQLTTEADECVLRFSVRDTGP
                     GIAPEKQDVLFDEFTQV"
BASE COUNT          501 a          850 c          946 g          449 t
ORIGIN      
        1 ggccgtgcgg tagagaccga catcgtgcgg gcggagcagg aaatacggca ggcaggaaag
       61 cgccaccagt cccgctccca ggaagacgcg ccgccagcgc cccagccgca gccagggaaa
      121 ggcgaagaag gcgggatagt tcttcagcgc caccgaggcg ctccagcacc agagagaggg
      181 ttgccggagg agcgcacgga atctctcctt cggccccgcc tgtggccatg actccggcgg
      241 cccctctcct tcgccggcgg cgggaatacg gccaccgccc tcggccccgg cccgcaactc
      301 cggccagaat gtcacccaga gaagcacggc catgaggagg gagaactgtc ccatccacag
      361 ctccaggtag aggggactga agccgagcgt cagtccggcc agggcacggc gcagagcgga
      421 aggaggcgat cgcaggcgcc tgaggctgaa aagcgtaaga ccgaggagcg cttcgttgat
      481 caccacccag acccagtagg agggccaggg cttcagcagc agcgcggtca gggccatgaa
      541 cgtggccgtg gggggtagat agcgatagaa gtagtagtaa ggcacgtggc gatcggaatc
      601 ctccggtctt ccaccttcca gatagatgct gcgcccctgg gtgaggttgt ccccggcctg
      661 atagatgccg aagtagtccg aagcctgccc gtagtcggtg gtggcctcga agaagagggg
      721 atccagccag tgggtgagaa ggctgacgag aaagaggaga tggacggcgc agccgatgta
      781 gaagatgacc agaccgggga ttcggccgag atccgaatcc tcccgcaccc tggtcccggt
      841 agtggtcacg gatgtctcct cgggtcgtga aatccggcgg gacggtcgcc gtcacaccgg
      901 tttccggcgc cgaaccggat tggctgagcc cgcgagccgg ggcgggctgt tccgtcgcga
      961 gacgcagcat agcgaaatcc cctcgacggc ggaagcgctc cggacgggcg tccgggccgg
     1021 tctccgtcga ggtcgctcgg gtgcgagctc cgaacttgtt ccggtccggc cacaccatcg
     1081 aggagatagc gaacggccgg ctggcgccca ggcgtacgtc ggaagtccga gctgagtcct
     1141 caacacattg tcctgaagcc acttgtcgcg tcgccacggg tctggttccg gaacgcctgg
     1201 ggagtaaagt ctctcctatc ccggaccgat atggagagtg tctgtattga gtgcggacct
     1261 gagagcatcc tggtgggatt ctgtcccgtc ggcatcaagg cgctcggttc tccgcgtggg
     1321 actttgtcgg tcaaggagag tgacttggcg tcaggaagaa gacccgatat ccgctcgagt
     1381 gaggacacga cacgcaaggc gttgcgcgaa agcgatcgaa acttccagac cttcttcgac
     1441 tccatgggtg agatggccct ggtgtcccgt cccgagggcc ggatcctgcg aaccaatccg
     1501 gagcttcggc ttcgtctggg gttcacggag cgcgagctcc ggcgcatgtc gcttgcggat
     1561 ctgcagccgc cggaccagcg ggcggacgct gccgtgctcc tggagtgtct catccgtggc
     1621 gatgtcgagt cggcgccgct tccgctgcag acccggcacg gcgcggtcat gggagcggac
     1681 gcccgggcct ggcggggaag atggaactcg cagccgtgct tcttcgtgct ctttcgggac
     1741 ctttccctgg agcaggaggc gcagcaacgc tttgaacggc tcttccgcag caaccccgca
     1801 ctgatggcgg tctcgagctt tcccgacggc tgtttcctgg atgtcaacga ggccttcctg
     1861 cagcggacgg gctaccgccg tgacgagctc atcgggcata ccagccagga gctggggttg
     1921 tttctccgcc ccgaccagca cgcgaccatg gttgagcggc tgcgccgcca gggccggctg
     1981 gtcaacgcgg agatgcagat ccgttgccgg aatggtgaga tccgggacgg gctcttctcc
     2041 ggtgaactgg tgcaaagcca ggggcggatt cacttcctga ccgtcatggc ggacatcacc
     2101 gacctcaagc gggccgacca tgagctgcgc gggactgtcg gtcgtctgga agaggccacg
     2161 gcgcgggccc gtgagctggc ggagacggcc gcgcaggcca gcctggccaa gagtgaattc
     2221 ctggccaaca tgagccatga gatccgaacc cccatgaacg gggtcatcgg catgaccgga
     2281 cttctgctgg ggacatccct ggacgcggag cagcgccgat acgccgaaac catccgccgg
     2341 agcggggaga gcctcctggc gctggtcaac gacatcctcg actattccaa gatggaggcc
     2401 cgcaagctcg atctggaatc gctcgacttc gacctgtcgg tcctgctgga cgacttcgcc
     2461 gacagcctcg ccctccgggc tcacgagcgg ggactggaac tcgtggtctg tccccagccg
     2521 ggggtgtccg ttctcctgcg cggcgatccg gggcgactgc ggcaggtatt gaccaatctg
     2581 gtgggtaatt cgctcaagtt caccgaacgg ggagaggtcg tggtcacggt cggccagctc
     2641 accaccgagg cggacgagtg tgtcctccgc ttcagcgtgc gcgacaccgg ccccgggatt
     2701 gcgccggaga agcaggacgt cctcttcgat gagtttacgc aggtgg
//
LOCUS       DASRZS010000157         7839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_859711, whole genome shotgun sequence.
ACCESSION   DASRZS010000157 DASRZS010000000
VERSION     DASRZS010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7839
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859711"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VFH83_03885"
     CDS             <1..988
                     /locus_tag="VFH83_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6485535.1"
                     /translation="SFGQIQQMFKVPDLLTDLSSEPWVSRLVDVSAVTDSGKVYGFPT
                     SLPPVEGVVYNKKIFQQVGVSVPKTYDELLAVCAKIRAAGIDPFTITAKDVWTVGMWV
                     VEMVPLAVKDKPGIWNDLNTGKVKFDSIPAFTDVFTKMKQIVDKGYANKDMFSAGYDI
                     GNDQVANGQAAMEVQGEWAANDIVKKYPDAQVGIFPLPLVDNSVFTSGFVAAFTIPQK
                     SKDIEASKGLIRYFADPAQVKIMAGDGYVPPAVKDVSVPLPPWTQEALTNYALKGKPP
                     IAEMGVASLVSMGQLSTYTTSMLDGTMKVEELMPAWAKYFGEQAAIRKLPGW"
     gene            1076..1951
                     /locus_tag="VFH83_03890"
     CDS             1076..1951
                     /locus_tag="VFH83_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018750140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485536.1"
                     /translation="MRNRRPSLYHPLLVAPGFLVYSVFFLLPVVVGFYYAFTDWNMYL
                     DRISFNGFTNFRRMFVDPISAGSLSHTLIFAVCTTIGKNVFGLALALALNQAVRTRNT
                     LRAVFFSPAVLSLIVVGLLFSSILYPDGILNVFLRTVGLGALARSWLADRTIVMYTIS
                     FVEIWQWSGFHMAIYLAGLQAIPKEFIEASVIDGVNGWQKFARITMPLIISTFNINLV
                     ISLIGGFKVFDLVFILTNGGPGRASEVINTQIYRVFGNGEWGFGTAMNLVLFLVITLI
                     ALTVLNLLRRREVEL"
     gene            1948..2784
                     /locus_tag="VFH83_03895"
     CDS             1948..2784
                     /locus_tag="VFH83_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485537.1"
                     /translation="MTVSARARRALGRGALAVVLWVVSLVILIPFWMMLIDSFKDARE
                     SALPSIALPSTWHFENYAVVFQRGGIGRSLANSVLVTGSSVLLTAVVAAAAALYLARK
                     STRSSRLIYNAFSIGLIAPLSIIPTIKLMQFLHINNTYGSVIVLMCAVNLPVAVFLCT
                     AFVKTIPRELDEASSIDGSNSFTLLWRVILPLMQPVIMTVAILVFMAVWNSFMIPLYF
                     LNNSARWTLPLTVYGFFGRYSHDWNLVDADLIITALPVVIFYMISQRYIISGMTAGAV
                     KG"
     gene            complement(2805..3086)
                     /locus_tag="VFH83_03900"
     CDS             complement(2805..3086)
                     /locus_tag="VFH83_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485538.1"
                     /translation="MPFSERLSTQRREVFESVFSETRGMPQVVRAATALSAFLDQKEI
                     FLSADDLLAGHEQYYDYRVPSEEEPASTPDERALPLQEEIIARTEHAAI"
     gene            complement(3073..4197)
                     /locus_tag="VFH83_03905"
     CDS             complement(3073..4197)
                     /locus_tag="VFH83_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485539.1"
                     /translation="MDRRERVMRQIRGQAVDRVPLMGGWFHGVDNLAALAGVSAERYL
                     ESPVENLARANLALDVDCMIEPIVPTYRGEIRTGEVLDARHAETTAEDLLRDGEAVPP
                     TEAGVLAAYDAAAEERKYREYFRRWTNALNPLILVPNFWDSVPDFHLFDRYGYNAYFS
                     AIALYPEAVGRIFWKMAVEARARNTIILRLIRELELPPMLFTGSDICNTRGPMVSIAF
                     LRQHYFPHERFALQPLVEAGIRVIRHCDGNVMPVVDDFITIGYSGFQGFQYECGVDPY
                     QLAARRSSRGERPLFFAGLNVTRTLPFGTQEDVRREVEYVFDFTDGGAGLFFFTSSSI
                     GPEVPLANVRAGYQYAAAGAGGEARAKHARWPGGARDAVL"
     gene            4315..5574
                     /locus_tag="VFH83_03910"
     CDS             4315..5574
                     /locus_tag="VFH83_03910"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6485540.1"
                     /translation="MRWRVDEHVAAWGTVAVALICRLTALMDFWRETATAGYPAQWPT
                     QFTFLILLSIAVAIVCGLFHDRALALFSVFVLFVIDIVLSVPESDRIVLRFLLQGSDI
                     LIAMLCLRPYLNVVFSGLLVIAIYFLFSPVLVFGVPVLAPAIAMRLGFCAVLLLCGAL
                     GLGLKLYIEESGRRSEELSRLDTTITQLTRANIGFQEYAKDVAERSAETERKRISRDI
                     HDTIGYTLTNIIMMTQEARLKALPFDEALSSLLETTRIQAQEGLRQTRRALRALRELV
                     PGKLNIHQTVTKLAAAFANATGVEVTVEFGNMTAAVEEDTSWVIYRMVQEGMTNALRH
                     GKATRIRILFWIDDDGYLMVDLLDNGRGSVGFEPGIGLSGMQERIEARRGRFQAGTVA
                     GGFRLTAWFPAGKDSDDEQDHQDRTGG"
     gene            5537..6205
                     /locus_tag="VFH83_03915"
     CDS             5537..6205
                     /locus_tag="VFH83_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6485541.1"
                     /translation="MTSRTIKIVLVDDQVLFVESLRRVLENLDPTLVVTGVAHDAREA
                     IEFVSSTGPDIVLMDVRMPGMDGVEAVRYIHERFPDIRIIMLTTFDDDEYVLSALEHG
                     AVGYLLKDIEPEKLIDSIRAVHSGSVLMSEAVASKLTRLTHGDGRGSTRPKPEWFQRL
                     SARERELLRHMVGGLSNREIASAMFIAEQTVRNHISEIYAKIGENDRARIIREYRSLF
                     PEDR"
     gene            6518..>7839
                     /locus_tag="VFH83_03920"
     CDS             6518..>7839
                     /locus_tag="VFH83_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6485542.1"
                     /translation="MRRTKLICLILCVALLATAGTVFAQSGKAAFAYDPSKPVNGGKA
                     ISIKFWTWNGFDYLYKAWAEDYQKIHPNVKIDFTVTPWDDYWKKLPLAISSGQGPDVF
                     AMHNQQTSLVIDGNLAAPYPADWKAGLESDFDFVDSHLIKGNLYYIDSGIMTSMVFYN
                     KKMWAAAGLTDKDIPTTWDQLTAVAKKLTKFDSSGKMVQEGFGLNGYAGYAVDMLSYQ
                     LGSTIYKPGGRTVVVDSAHKKALQRVIDWFDKDKISSRDFPNNNESFAQEKTAMIYMW
                     GWFNGYLVANNPNIQFGIFKLPTWDGKTPPAYDRNNGESTFSVNPKISATNKAVAFDF
                     IHYCIENEDYNRQIALKGSQAPVMKRIKEDKELLADPAVGVTLQELDRTLWPGAFSTG
                     WDPIKTKTVVDGVKTGLGVDQILSMYESQNNSDLAKYKNVYWDQERVY"
BASE COUNT         1418 a         2610 c         2432 g         1379 t
ORIGIN      
        1 ttcctttggc cagatccagc agatgttcaa ggtgcccgac ctcttgacgg acctgagctc
       61 cgagccgtgg gtcagccggc tggtcgacgt ctccgcggtc acggacagcg gcaaggtcta
      121 cgggttcccg acgtcccttc cgcccgtgga aggcgtcgtc tacaacaaga agatcttcca
      181 gcaggtggga gtctcggtcc caaagaccta tgacgagctc ctggccgtct gcgccaagat
      241 ccgcgccgcc gggatcgacc cgttcaccat cacggccaag gacgtctgga cggtgggcat
      301 gtgggtcgtg gagatggtgc ccctggccgt caaggacaag ccgggcatct ggaacgatct
      361 gaacaccggc aaggtgaagt tcgactccat cccggcgttc acggacgtgt tcacgaagat
      421 gaaacagatc gtggacaagg gctacgccaa caaggacatg ttctccgccg gctacgacat
      481 cggcaacgac caggtcgcca acggccaggc ggccatggaa gtccagggtg agtgggccgc
      541 caacgacatc gtcaagaagt atccggacgc ccaggtaggg atcttccccc tgccgctcgt
      601 cgacaactcc gtgttcacgt ccgggttcgt ggctgccttc accatcccgc agaagtccaa
      661 ggacatcgag gcctccaagg gactcatccg gtatttcgcg gaccccgcgc aggtgaagat
      721 catggccggt gacgggtacg ttccgccggc ggtcaaggac gtgtcggttc cgttgccgcc
      781 gtggacgcag gaggccctga ccaactatgc cctcaagggc aagccgccga tcgcggagat
      841 gggcgttgcc tccctggtgt ccatgggcca gctctccacg tacaccacca gcatgctgga
      901 cggaaccatg aaggtggaag agctcatgcc ggcatgggcc aagtacttcg gcgagcaggc
      961 ggccatccgc aagctgccgg gctggtagcc ctcggcaccg atcgacagca caagggcgga
     1021 tgccctcccc caggggggca tccgcctctc gcccctggcc cgtagagagc ccatcgtgag
     1081 aaaccgcagg cccagcctgt accacccgct cctggtggca ccgggcttcc tggtgtactc
     1141 cgtcttcttt ctgcttccgg tcgtggtcgg cttctattac gccttcaccg actggaacat
     1201 gtacctggac cgcatctcct tcaacggctt cacgaacttc cgccgtatgt tcgtggaccc
     1261 gatttccgcc ggctcgctct cccataccct gatcttcgcc gtctgcacga ccatcgggaa
     1321 gaacgtgttc ggtctggcgc tggccctggc gctcaaccag gcggtgcgca cgcgaaacac
     1381 cctgcgcgcg gtgttcttct ctcccgccgt gctcagcctc atcgtggtgg ggctcctctt
     1441 ctcctcgatc ctctaccccg acgggatcct gaacgtattc ctacggaccg tggggctggg
     1501 cgctctggcg cggagctggc tggcggatcg caccatcgtg atgtacacga taagcttcgt
     1561 cgagatctgg cagtggtcgg ggttccacat ggccatctac ctggccgggc tccaggccat
     1621 ccccaaggag ttcatcgagg cctcggtgat cgacggggtc aacgggtggc agaagttcgc
     1681 gcgcatcacg atgcccctga tcatctccac cttcaacatc aacctcgtga tctccctgat
     1741 cggcggcttc aaggtgttcg acctcgtgtt catcctcacc aacggcggcc ccgggagggc
     1801 gagcgaggtg atcaacacgc agatctatcg cgtgttcggc aacggggagt ggggcttcgg
     1861 gacggccatg aacctggtgc tcttcctggt catcacgctg atcgcgctga cggtgctcaa
     1921 cctcctgcgg cggcgggagg tggagctatg acggtctccg cccgagccag aagagccctg
     1981 ggacgcggag ctctggccgt ggtcctctgg gtggtgagcc tcgtgatcct catcccgttc
     2041 tggatgatgc tgatcgactc cttcaaggac gcgcgggagt ccgcgctgcc ctccatcgcc
     2101 ctgccctcca cctggcactt cgagaactat gccgtggtgt tccagcgggg cgggatcggc
     2161 cggtccctgg ccaacagcgt gctggtcacg gggtcctccg tcctgctcac ggccgtggtg
     2221 gcggctgccg ccgctctcta cctggctcgc aagagcacgc gttccagccg gctgatctac
     2281 aacgcgttct ccatcggcct gatcgcgccg ctctccatca tcccgaccat caagctgatg
     2341 cagttcctgc acatcaacaa cacctacggc tcggtgatcg tcctgatgtg cgccgtcaac
     2401 ctgccggtgg cggtcttcct gtgcaccgcc ttcgtgaaga ccatcccgcg cgagctggat
     2461 gaggcgagca gcatcgacgg ctccaactcc ttcacgctcc tctggcgcgt catcctgccg
     2521 ctcatgcaac ccgtgatcat gacggtggcc atcctggtgt tcatggcggt ctggaactcg
     2581 ttcatgatcc cgctgtactt cctcaacaac agcgcccggt ggaccctgcc cctcaccgtg
     2641 tacgggttct tcggtcgcta ctcccacgat tggaacctgg tggacgcgga cctgatcatc
     2701 acggcgctcc ccgtggtgat cttctacatg atctcgcaga ggtacatcat cagcgggatg
     2761 acggcgggag cggtcaaggg ataggcagcg ccaggacaac ccggctagat ggcggcatgc
     2821 tccgtacggg cgatgatctc ctcctggagc ggcaacgccc gctcgtcagg ggtcgacgcg
     2881 ggctcttcct cagagggcac ccggtagtcg tagtactgct cgtggccggc cagcagatcg
     2941 tccgcggaca ggaagatctc cttctgatcg aggaaagccg aaagggccgt cgcagcgcga
     3001 acgacctgag gcatgccccg agtctcggaa aagacgctct cgaagacctc ccgacgctgg
     3061 gtcgacagac gctcagagaa cggcatcccg agcccctcct ggccagcgcg cgtgcttcgc
     3121 ccgagcctct ccgcccgcgc cggcggctgc gtactggtac ccggcgcgaa cgttcgccag
     3181 gggcacctct ggaccgatgg agctgctggt aaagaagaag aggccggcgc ctccgtcggt
     3241 gaagtcgaac acgtactcca cctcccggcg cacgtcctcc tgagttccga acggcagggt
     3301 ccgggtcacg ttcaggcccg cgaagaacaa cggccgctct cctcggctgc ttcgccgggc
     3361 ggcgagctga tacggatcca cgccgcattc gtactggaac ccctggaaac ccgagtagcc
     3421 gatggtgatg aaatcatcca ccacgggcat cacgttcccg tcgcagtgac ggatcacccg
     3481 gattcccgcc tccaccagcg gctgcagcgc gaagcgctcg tgggggaagt agtgctgccg
     3541 caggaaggct atggagacca tggggccgcg cgtattgcag atgtcggagc cggtgaagag
     3601 catcggcggc agctccagct cccggatcaa ccggaggatg atcgtgttcc gcgcgcgggc
     3661 ctcgacggcc atcttccaga agatgcggcc caccgcttcc gggtagaggg cgatggcgga
     3721 gaagtaagcg ttgtacccgt accggtcgaa gaggtggaag tccggcacgg aatcccagaa
     3781 gttgggcacg aggatgaggg gattgagggc attcgtccac cggcggaagt actcccggta
     3841 cttccgctcc tccgcggcgg cgtcataggc cgcgagcacg cctgcctccg tcggcggcac
     3901 ggcctccccg tctcggagca ggtcctccgc cgtcgtctcc gcgtgtcggg catccagcac
     3961 ctccccggtg cggatctcgc cacgataggt gggcacgatg ggctcgatca tgcagtcaac
     4021 gtccagggcg aggttggcgc gcgccagatt ctcgacaggg ctctccaggt atcgctccgc
     4081 ggataccccg gccagcgcgg cgaggttgtc gacgccgtga aaccacccgc ccatgagagg
     4141 cacgcggtcc accgcctgcc cgcgtatctg acgcatcacc cgctcgcgcc ggtccatcag
     4201 gtcgagctcc ttccgtctca gggatttgga cgggctggat tcctatcccc cgcgccgcgg
     4261 cgtgtcaagg ggaccctgga cacgcccccc cgatgcccct atccttttcg cctgatgcga
     4321 tggagggtcg atgagcacgt cgccgcctgg ggcaccgtag cggtggcgct catctgccga
     4381 ctgacggcgc tcatggattt ctggcgggag acggcaacgg ccggataccc cgcccaatgg
     4441 cccacccagt tcacgttcct catcctcctg tcgatcgcgg tggcgatcgt ctgcgggctc
     4501 ttccatgacc gcgccctggc gctgttctcc gtgttcgtgc tcttcgtgat cgacatcgtt
     4561 ctcagcgtcc cggaatccga ccgcatcgtc ctgcgcttcc tgctgcaggg ttccgatatc
     4621 ctgatcgcca tgctctgcct ccgcccctac ctgaacgtgg tgttctccgg cctcctggtg
     4681 atcgccatct acttcctctt cagcccggtc ctcgtgttcg gggtccccgt gctggccccc
     4741 gccattgcga tgcgtctcgg gttctgcgcg gtgctcctgc tctgcggagc gctgggtctc
     4801 gggctgaagc tgtacatcga ggaatccggg cgacggagcg aggagctctc ccggctggac
     4861 accaccatca cgcagctcac ccgcgccaac atcggcttcc aggagtacgc caaggacgtg
     4921 gccgagcgat ccgcggagac cgagaggaaa cgcatctcgc gggacatcca cgacaccatc
     4981 ggctacaccc ttaccaacat catcatgatg acccaggagg cgcggctgaa ggccctgccg
     5041 ttcgatgagg cgctgtcctc cctcttggag accacgcgga tccaggcgca ggagggactg
     5101 cgccagacgc ggcgcgccct gcgcgcgttg cgggagctgg tcccggggaa gctcaacatc
     5161 caccagaccg tcaccaagct ggccgccgcg ttcgccaacg ccacgggggt cgaggtgacg
     5221 gtggagttcg gcaacatgac ggccgccgtg gaggaggaca cctcctgggt catctaccgt
     5281 atggtccagg agggcatgac gaacgccctc cgtcacggga aggcgacccg gatccggatc
     5341 ctgttctgga tcgacgacga tggttacctc atggtggacc ttctggacaa cggtcgcggc
     5401 tccgtggggt tcgagcccgg gattgggctt tccggcatgc aggagcgcat cgaggcccga
     5461 aggggccggt tccaggcggg gacggttgcc ggcgggttcc ggctgaccgc gtggttcccg
     5521 gcgggaaagg attcggatga cgagcaggac catcaagatc gtactggtgg atgaccaggt
     5581 cctcttcgtc gagagcctgc gacgggtcct ggagaacctg gatcccacgc tggtcgtcac
     5641 cggggtggcg cacgacgccc gggaagccat cgagttcgta tcgtcgacgg gcccggacat
     5701 cgtgctcatg gacgtgcgga tgccgggcat ggacggcgtg gaagcggtcc gctacatcca
     5761 cgaacggttc ccggatatcc ggatcatcat gctcacgacc ttcgacgacg atgagtacgt
     5821 cctgagcgcc ctggagcacg gcgcggtggg gtaccttctc aaggacatcg agccggagaa
     5881 gctcatcgac tccatccggg cggtgcactc ggggtcggtg ctcatgtcgg aggcggtggc
     5941 atccaagctc acgcggctca cccacgggga cggccgcggc tccacccggc ccaaaccgga
     6001 atggttccag cgtctcagcg cgcgggagag agagctcctg cggcacatgg tgggcggatt
     6061 gagcaaccgc gagatcgcca gtgccatgtt catcgcggag cagaccgtcc gaaaccacat
     6121 cagcgagatc tacgccaaaa tcggcgagaa cgaccgggcc cggatcatcc gggagtatcg
     6181 gtcgctgttt cccgaggatc gctgagtcgt ctcccgaggc ggcagtaccg cctagtaccg
     6241 aaaaaggtac aaaaacagta ccgcaatacc tttcgctccg accgcgcgag gctctatact
     6301 ggcggcacca catccagaac caacgacaaa cggggcgttt ccaccggttc ccgactcgag
     6361 cgagggggcg gtgcagaccg cccggcattc caagacgccc ccgcccgccg ccgcgcgggg
     6421 gttgcggaca aaggagggaa aggcttccga cggctcgcgc ttccgggatc gaccggatcg
     6481 ttgcccggcc ccgacatcaa ggacgaaagg agcttccatg agaagaacca agctgatctg
     6541 cctcatcctc tgcgttgcgc ttctggcaac cgcggggacg gtcttcgccc agagtggcaa
     6601 ggccgcgttc gcctatgacc cttccaagcc cgtgaacggc gggaaggcca tctcgatcaa
     6661 gttctggacc tggaacgggt tcgactacct ctacaaagcg tgggcggagg attaccagaa
     6721 gatccacccc aacgtcaaga tcgacttcac ggtgaccccg tgggacgact actggaagaa
     6781 gctgccgctc gccatctctt ccggtcaggg accggacgtg ttcgccatgc acaaccagca
     6841 gacctccctc gtcatcgacg ggaacctggc ggccccgtat ccggcggact ggaaggccgg
     6901 cctcgagagt gacttcgact tcgtcgactc ccacctcatc aagggcaacc tctactacat
     6961 cgactccggc atcatgacct ccatggtctt ctacaacaag aagatgtggg cggccgccgg
     7021 tctgacggac aaggacatcc ccaccacgtg ggaccagctc accgcggtgg ccaagaagct
     7081 gaccaagttc gactcctccg gcaagatggt ccaggagggc ttcggcctga acggatacgc
     7141 cgggtacgcg gtggacatgc tctcctacca gctcggctcc acgatctaca agccgggtgg
     7201 acggaccgtc gtcgtggact ccgcccacaa gaaggcgctg cagcgcgtca tcgactggtt
     7261 cgacaaggac aagatctcct cgcgcgactt ccccaacaac aacgagtcct tcgcccagga
     7321 gaagaccgcg atgatctaca tgtggggctg gttcaacggc tacctggtcg ccaacaaccc
     7381 caacatccag ttcggcatct tcaagctgcc cacctgggac ggcaagacgc cgccggccta
     7441 tgaccgcaac aacggcgagt ccaccttctc ggtgaacccg aagatctccg cgaccaacaa
     7501 ggccgtcgcc ttcgacttca tccactactg catcgagaac gaggactaca accggcagat
     7561 cgccctcaag ggctcgcagg cgccggtgat gaagcggatc aaagaggaca aggagctgct
     7621 cgcggatccc gcggtcggcg tcaccctgca ggagctcgac cggaccctgt ggccgggcgc
     7681 cttctccacg ggctgggacc ccatcaagac gaagaccgtt gtagacggcg tcaagacggg
     7741 cctgggcgtg gaccagatcc tgtccatgta cgagagccag aacaactcgg atctggccaa
     7801 gtacaagaac gtgtactggg accaggagag ggtctacaa
//
LOCUS       DASRZS010000158         7172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5089207, whole genome shotgun sequence.
ACCESSION   DASRZS010000158 DASRZS010000000
VERSION     DASRZS010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5089207"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFH83_03925"
     CDS             complement(<1..418)
                     /locus_tag="VFH83_03925"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HET6485543.1"
                     /translation="MSDSGRIIQAARRLKLAVPAFNVPYLPMVEPVVRAIRDEDAFSF
                     VEVARLELFKFEARSLRAVKEEYDRYADPARVRLHLDHVPVLDEDGITVDYLPVIQEA
                     IQLGYDSVMVDGSRLTLDENIAATRRVVEAAAKAGIP"
     gene            complement(484..1344)
                     /locus_tag="VFH83_03930"
     CDS             complement(484..1344)
                     /locus_tag="VFH83_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6485544.1"
                     /translation="MPRVLRLANLSDGFPLVVDRIRHHGATSLHTHDFSELVIILAGR
                     GVHYSTDTAYPVIAGDAFVVTQAHGYRETEDLDLINVLFDAQRLALPLEDARKLPGYH
                     AFFALEPRFRRRHGFRSSLHLSLAELAVVSELVAQMEAELRKKSSGYELMTKSLMLQL
                     IGRLSRAYERMRAVESRPLIRLGTVLSHLERHYAEEVRLEDLAEIAGTSTSSLQRAFR
                     AITGHAPIEYLIRLRLLRACELLRKGELNVTETAYRVGFTDGNYFSRQFRRVMACSPR
                     EFVNRARLAG"
     gene            1455..2948
                     /locus_tag="VFH83_03935"
     CDS             1455..2948
                     /locus_tag="VFH83_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6485545.1"
                     /translation="MFSSRAPARRPVHPSWLVVLVVLCVAFAAFSTPRAAAAPVKLRM
                     FAWEYTPRDRAPSDRWDPPSYLRTLRDSYQRLHPDVSIEFLPYIPLGPDYDTWFVTQF
                     KAGNAPDVGNQLFSEVNRQSNKGWFLDFTPYLDKPDPYVPGNQHWKDIFLPGVITTGT
                     APDGKIYVLPTGITGTALYYNKDIFRKAGVSVPQTWKEFMDVQQKIKDAGFIPFAFNM
                     SGARYASNWALRCLQDMLLDSKLSQIKGVSGPIPRTMTEGSGISQKELVAAIKKGTYS
                     AMDPQWQEQLRLLKEWSKYWDPGFLSLDQNGAYTEFVTGKVAMFWDSSAKVKPLSVDP
                     LRTFDFGTFSFPKVTKESSRYATEISAPAIGGFTANGGDYIVDALAVQRGTADASIDW
                     LMYITAPQNYVPLANDLGSYAPGLKEVKSLDPSLAPFIEAAQKGVFRIENYLRGLTVK
                     YADQYYQTLEEYLAGRKDLPTACAEIQKYMNQAADDLISANGWTDLQ"
     gene            2959..3876
                     /locus_tag="VFH83_03940"
     CDS             2959..3876
                     /locus_tag="VFH83_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485546.1"
                     /translation="MKTKVVALASPPALISLLLVVPAFLVLAVFRYYPVISALWHSLY
                     QWDGFRVGRYIGLANYRELLADPLMGAATTNIVRYVVIRVVLNLVFPLLAAELVFHLG
                     KGRGQSLYKGLFTVPLVVPLMVVLLVWKFIYNPNDGLLNRLLGLVGLGGAARDWLGGF
                     DTALYAISGLGFPWVTGIGISGFAMLLYLAALQAIPGELYDAGAVDGIRGAQRLFALE
                     LPLIGRQLWLVALLTVINTLQSYVPVMVLTMGGPGSASLVPGLYLYLNAFSYDRFGYA
                     CAVGVLMAVVLAVLAWLYTRVSRLGEEAA"
     gene            3873..4700
                     /locus_tag="VFH83_03945"
     CDS             3873..4700
                     /locus_tag="VFH83_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485547.1"
                     /translation="MSGRRAHRAAAHFVLILLAVASLYPFWFLLMTSLKSNDQFYHAY
                     YAPTLPFHWSNYREAWNQISGYFVNSITVTAVSVLASVVLASLAAYAFARYRFPGSGA
                     LYLLILVVLLIPGTLTLIPAFVILRNLHLIGTHRALYAMYVADSQVLGILILRGFFAG
                     VPEELLEAASLDGAGEVQTMARVVAPLARPALLTVAIMTALSSWNDYLWPLIVLPDTR
                     KWTVTLGLVSFRDRYAGMSAWGPLFAGFAIASVPLFVLFFASMRHFVSGLTSGAVKA"
     gene            4746..5546
                     /locus_tag="VFH83_03950"
     CDS             4746..5546
                     /locus_tag="VFH83_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET6485548.1"
                     /translation="MIKLGVNSVLFGGFDFPTAARHIAMAGYDGIELSAIKGMCEHLV
                     LDRWREQVDAIRKTTADNKLALLSMEVTSLEDDRAIPGIEAAQALGIPVVNFGPGGKS
                     KNKEDLDRQAGIIARLAEIAARHGVTLCVKAHVGACVDDTPTTQELMKRVQGPGFGVD
                     MDPSHVYRAGEDPAKALSSVIQRVRHIHIRDCKGKGPGPGTPPDQACGRGDIDLGAFC
                     KVMADAGYDGPVCLEVIGAGQMELAQVSVIAAESYGYLNAVLKRLGKR"
     gene            5567..6988
                     /locus_tag="VFH83_03955"
     CDS             5567..6988
                     /locus_tag="VFH83_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET6485549.1"
                     /translation="MPNRKTNLIFFGIDSLRPDHTSLYGYRRLTTPHMDRYLKDGTVF
                     ENVLSPHIPTTPGYSSMFTGMDAFGTDCVALRHKGQLGSHVATMAEVLGEQGYETVCV
                     GFSGNPASRGFQKYVDFSGWGSWMEGRSPKAENLNAVALPELVRMANAKKPFFLFLRH
                     MDPHAPYLPPRPFERMFYHGNEMDPANRSLDPVYSFKPFCDFFKSWFPPGCTDKDYII
                     AQYDGEVAYMDACIEQLLTTIATLGLEEDTLVVFDSDHGETHYDHGCHFDHHGLYECT
                     VRIPLAFRMPGRVAGGRRYGEPCQMKDIAPTVYDLMGIKTNIRFDGRSLVPLMEGTER
                     VSEPEAYITECTWMRKHGWRTPEWKLIHALEPDFHHKPEVELYNLVSDPGENTNLADK
                     EPEVVKWLEARMQAHIQRREKETGRTNPMYTNLDWHGQGVGPFKTSDQAYNTLYIGSP
                     QAARELQEKALKELEAQRGARPL"
     gene            6985..>7172
                     /locus_tag="VFH83_03960"
     CDS             6985..>7172
                     /locus_tag="VFH83_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6485550.1"
                     /translation="MSAASSTAGGRTAGADGAVRVGVIGCGPIGNLHARLYAGMDHAR
                     LVGVCDVLPERARACGELL"
BASE COUNT         1199 a         2409 c         2346 g         1218 t
ORIGIN      
        1 aggggatccc ggccttcgcc gccgcctcca ccacccttcg cgtggcggcg atgttctcat
       61 ccagggtgag gcgggatccg tcgaccatca ccgagtcgta gccgagctgg atcgcctcct
      121 ggatgaccgg caggtagtcc acggtgatcc cgtcctcgtc cagcacgggg acgtggtcca
      181 ggtggagacg gacacgcgcg ggatccgcgt aacggtcgta ctcctccttc accgcccgaa
      241 ggctgcgcgc ctcgaacttg aagagctcca gacgcgccac ctcgacgaag gagaacgcat
      301 cctcgtcccg gatggcccga accaccggct ccaccatcgg caggtacggc acgttgaacg
      361 ccggtaccgc gagcttgagc ctgcgcgccg cctgaatgat cctcccgctg tcactcaccc
      421 tggtacacct ttcctccgcg cgcccgttgc cgccgggaca ccgcccacca tccctgcacg
      481 gggctacccg gccaggcgcg cgcgattgac gaactcccgc ggactgcacg ccatgacccg
      541 gcggaactgc cggctgaagt agttgccgtc cgtgaacccc acgcggtagg ccgtctcggt
      601 cacgttcagc tcccccttgc gcagcagctc gcaggcccgc agcagacgca ggcggataag
      661 atactcgatg ggcgcgtgtc cggtaatggc ccgaaacgcg cgctgcaggc tgctggtgga
      721 ggtgccggct atctctgcca gatcctccag ccgcacctcc tccgcgtaat gccgctccag
      781 gtggctcagc acggtcccca ggcggatgag agggcgggac tccaccgcgc gcatgcgctc
      841 gtaggcgcgg gagagccggc cgatgagctg cagcatcagc gacttggtca tcagctcgta
      901 cccggagctc ttcttccgca gctccgcctc catctgggcg acgagctccg agaccacggc
      961 gagctccgcc aggctgagat gcaggctgct tcggaagccg tgcctgcggc ggaaccgagg
     1021 ctccagcgcg aagaaggcgt ggtagccggg aagcttccgc gcatcctcca ggggcagggc
     1081 cagccgctgc gcgtcgaaga gcacgttgat caggtcgagg tcctcggtct cccggtaccc
     1141 gtgcgcctgg gtaaccacga aggcgtcacc ggcgatcacc gggtaggcgg tgtccgtgct
     1201 gtagtggacc ccgcgcccgg ccagaatgat gaccagctcg gagaagtcat gggtgtggag
     1261 ggaggtggcg ccgtgatggc ggatgcggtc gaccaccagg gggaagccgt ccgacaggtt
     1321 ggccagcctc agcacgcgcg gcacgggtcg ctccaggctc tcatggcgtg accagatcgt
     1381 gctactattt gccgcaagcg tcaaggcgcg ccggtcgggt ttctggtata ctgaccgcca
     1441 ggaggtgggt ccgtatgttc agttctcgag ccccagcgcg gcggcccgtg cacccgtcct
     1501 ggctcgtcgt gcttgtggtc ctctgcgtcg cgttcgcggc gttctccacc ccgcgggcgg
     1561 cggccgcccc ggtgaagctc cgcatgttcg cctgggaata cacgccgagg gaccgagccc
     1621 cctcggaccg atgggatccg cccagctacc tgcggacgct gcgggactcc tatcagaggc
     1681 tccacccgga cgtcagcatc gagttcctgc cgtacatacc gttgggtccg gattacgata
     1741 cctggttcgt gacccagttc aaggccggga acgctccgga cgtcggcaac cagctcttca
     1801 gcgaggtcaa tcggcagtcg aacaagggat ggttcctgga cttcacgccg tacctggaca
     1861 agccggatcc gtatgtcccc ggcaaccagc actggaagga catcttcctg ccgggagtca
     1921 tcaccacggg aaccgctccg gacgggaaga tctacgtgct gcccacgggc atcacgggga
     1981 ccgccctcta ctacaacaag gacatcttcc gcaaggcggg cgtctccgtc ccgcagacgt
     2041 ggaaggagtt catggacgtc cagcagaaga tcaaggacgc cgggttcatc cccttcgcgt
     2101 tcaacatgag cggggcgcgg tacgcctcca actgggcgct gcgttgcctt caggacatgc
     2161 tcctggactc caagctctcc cagatcaagg gcgtgagcgg accgatcccc cggaccatga
     2221 ccgaggggtc cggtatcagc cagaaggagc tcgttgcggc catcaagaag ggcacctact
     2281 ccgcgatgga tccccagtgg caggagcagc ttcggctcct caaggagtgg tcgaagtact
     2341 gggaccccgg gttcctcagc ctggatcaga acggcgccta cacggagttc gtgaccggaa
     2401 aggtcgccat gttctgggat tcctccgcca aggtgaagcc gctgagcgtg gacccgcttc
     2461 ggaccttcga cttcgggacc ttcagcttcc ccaaggtcac caaggagagc tcgaggtacg
     2521 ccacggagat ctccgccccg gctatcggcg ggttcaccgc caacggtggg gactatatcg
     2581 tggacgcgct cgcggtccag cgcggcacgg cggacgcctc catcgactgg ctcatgtaca
     2641 tcaccgcgcc ccagaactac gtgccgctgg ccaacgacct ggggtcctac gcgccgggcc
     2701 tcaaggaggt gaagtccctg gatcccagcc tggcgccgtt catcgaggcc gcccagaagg
     2761 gggtgttccg gatcgagaac tacctccgag ggctcacggt gaagtacgcc gaccagtact
     2821 accagaccct ggaggagtac ctggccggac gcaaggacct gcccacggcc tgcgccgaga
     2881 tccagaaata catgaaccag gccgcggatg acctgatctc cgccaacggg tggacggacc
     2941 tgcagtaggc ttcctctcgt gaagacaaag gtggtggccc tggcctcccc gccggcgctg
     3001 atcagcctgc tgctggtggt gccggcgttc ctcgtcctcg cggtcttccg ctactacccg
     3061 gtgatctccg ctctctggca ctccctgtac cagtgggacg gctttcgcgt cgggcggtac
     3121 atcgggctcg ccaactaccg tgagctgctc gccgatccgc tgatgggcgc cgccaccacc
     3181 aacatcgtcc gctacgtcgt gatccgcgtt gtcctcaacc tcgtcttccc gctcctcgcc
     3241 gcggagctcg tcttccacct gggcaagggc cgcgggcagt ccctgtacaa ggggctcttc
     3301 acggtgccgc tggtggtgcc cctcatggtg gtcctgctgg tgtggaagtt catctacaac
     3361 ccgaacgacg gcctcctcaa ccggctcctg ggcctggtcg gcctgggcgg agcggctcgg
     3421 gactggctgg gcgggttcga caccgccctc tatgccatca gcggtcttgg cttcccgtgg
     3481 gtcaccggca tcgggatctc gggattcgcc atgctcctct acctcgccgc cctgcaggcc
     3541 atccccgggg agctgtacga cgctggcgcg gtggatggca tccggggcgc tcagcggctt
     3601 ttcgcgctgg agcttcccct gatcgggcgg cagctctggc tggtagcgct cctcacggtg
     3661 atcaacacgc tccagagcta cgtgccggtg atggtcctga cgatgggagg acccggctcc
     3721 gcctcgctgg tacccggtct gtacctgtat ctgaacgcgt tcagctacga ccggttcgga
     3781 tacgcgtgcg ccgtgggcgt gctgatggcc gtggtgctgg cggtgctggc gtggctttac
     3841 acccgcgtct cccggctggg ggaggaggcg gcgtgagcgg acgtcgagcg caccgggcgg
     3901 cggcccattt cgtcctgatc ctgcttgccg ttgccagcct gtatccgttc tggttcctcc
     3961 tcatgacctc gctcaagtcc aacgaccagt tctaccacgc ctactacgcg cccactcttc
     4021 cgtttcactg gtccaactac cgggaagcct ggaaccagat cagcgggtac ttcgtgaaca
     4081 gcatcacggt gacggcggtc tcggtgctgg ccagcgtggt gctggcatcc ctggcggcat
     4141 acgcgttcgc ccgctaccgg ttccccggca gcggcgccct gtatctcctc atcctggtgg
     4201 tgctgctgat ccccggtacg ttgaccctca tcccggcctt cgtgatcctt cggaacctgc
     4261 acttgatcgg gacccaccgt gcgctgtacg ccatgtacgt ggcggattcg caggtcctgg
     4321 gcatcctgat cctgcgcggc ttcttcgcgg gcgtcccgga ggagctcctg gaggccgcgt
     4381 ccctggacgg ggccggggag gtccagacga tggcgcgggt cgtggctccc ctggcacggc
     4441 cggcgctgct cacggtagcc atcatgacgg ccctttcgag ctggaacgac tatctttggc
     4501 cgctcatcgt gctgccggat acgcgcaagt ggacggtcac cctggggctg gtgtcctttc
     4561 gggaccgcta cgccgggatg tcggcgtggg ggccgctgtt cgcggggttc gccatcgcgt
     4621 ccgtcccgct gttcgtcctg ttttttgcca gcatgcgcca tttcgtgtcc ggcctcacct
     4681 cgggcgcggt gaaggcgtag aggaatgcgc ggctggaaga cccgctcgac ctgcaaggag
     4741 gtaccatgat caagctcgga gtgaactcgg ttctgttcgg cggattcgat ttccccaccg
     4801 cggcccggca catcgccatg gcgggctatg atggcattga gctttccgcc atcaaaggca
     4861 tgtgcgagca cctggtcctg gaccgctggc gcgagcaggt ggacgccatc cgaaagacca
     4921 cggcggacaa caagctggcg ctcctgtcca tggaggtcac ctcactggag gacgaccgcg
     4981 ccattcccgg catcgaggcc gcgcaggcgc tcggaatccc cgtggtcaac ttcggccccg
     5041 gagggaagag caagaacaag gaggacctgg accggcaggc gggaatcatc gcgcggctgg
     5101 cggagatcgc ggccaggcac ggcgtgaccc tgtgcgtgaa ggcccacgtg ggcgcctgcg
     5161 tggacgacac ccccaccacg caggagctga tgaagcgggt ccagggcccg ggctttgggg
     5221 tggacatgga ccccagccac gtctaccggg ccggcgagga tccggccaag gcgctttcat
     5281 cggtcatcca gcgcgtgcgg catattcaca tccgggactg caagggcaag gggccgggac
     5341 ccggcacgcc gccggaccag gcctgcggga gaggagacat cgacctgggc gcgttctgca
     5401 aggtcatggc ggacgccggt tacgacggcc cggtctgcct ggaggtgatc ggcgctggac
     5461 agatggagct ggcgcaggtc agcgtcatag ccgcggagag ctacggctat ctcaacgcgg
     5521 tgctcaagcg tctcggaaaa cggtagccat tgaaaggagg gccgaggtgc ccaatcgaaa
     5581 gaccaatctg atcttcttcg ggatcgacag cctgaggccg gaccacacga gcctctacgg
     5641 ctatcgccgg ctcacaacgc cccacatgga ccgctacctc aaggacggga ccgtgttcga
     5701 gaacgtgctg agcccgcaca tcccgaccac ccccggctac tcgtccatgt tcaccggcat
     5761 ggatgccttc ggaacggatt gcgtggcgct gcgccacaag gggcagctcg gttcccacgt
     5821 ggccaccatg gccgaagtcc tgggagagca gggatacgag acggtgtgcg tgggattcag
     5881 cggaaacccg gcctcgcgcg gcttccagaa gtacgtggac ttctccggct ggggctcctg
     5941 gatggagggg cgcagcccca aggcggagaa cctgaacgcc gtggcgctgc cggagctcgt
     6001 gcgcatggca aatgccaaga agcccttctt cctgttcctg cggcacatgg acccccacgc
     6061 cccctacctg ccgcctcggc cgttcgagcg gatgttctac cacggaaacg agatggatcc
     6121 ggccaaccgg tcgctggatc ccgtgtattc attcaagccc ttctgcgact tcttcaagag
     6181 ctggttcccg cccggctgca cggacaagga ctacatcatc gcccagtacg acggcgaggt
     6241 ggcctacatg gacgcttgca tcgagcagct cctcaccacc atcgccaccc tgggcctgga
     6301 agaggacacc ctggtggtct tcgactcgga ccacggagag acccactacg atcacgggtg
     6361 tcacttcgac caccacgggt tgtacgagtg cacggtgcgc atcccgctcg ccttccgcat
     6421 gccgggtcgg gtcgccggag ggaggcgcta cggggagccc tgccagatga aggacatcgc
     6481 ccccaccgtc tacgacctga tgggaatcaa gacgaatatc cggttcgacg gccgcagcct
     6541 ggtccccctc atggagggaa cggagagggt ctccgagccc gaggcctaca tcaccgaatg
     6601 cacctggatg cggaagcacg gctggcgcac gccggagtgg aagctgatcc acgcgctgga
     6661 gcccgacttt caccacaagc ccgaggtgga gctgtacaac ctggtctccg atcccgggga
     6721 gaacaccaac ctggcggaca aggagcccga ggtggtgaag tggctggagg cgcgcatgca
     6781 ggcgcacatc cagcggcggg agaaggagac gggtcggacc aatcccatgt acaccaacct
     6841 ggactggcac ggccagggag tcgggccgtt caagacttcg gaccaggcct acaacacgct
     6901 gtacatcggc tcgccgcagg cagcgcgcga gctgcaggag aaggcgctga aggagctgga
     6961 ggcccagcgc ggcgccagac cgctgtgagc gccgcgtcct caaccgcggg cggccggacc
     7021 gcgggcgccg acggagccgt ccgcgtaggg gtgatcggct gcggtcccat cgggaacctc
     7081 catgcccgcc tgtacgcggg catggaccac gcccggctgg tgggcgtctg tgacgtgctc
     7141 ccggagcgcg cgcgtgcctg cggcgagctc ct
//
LOCUS       DASRZS010000159         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774 k141_88262,
            whole genome shotgun sequence.
ACCESSION   DASRZS010000159 DASRZS010000000
VERSION     DASRZS010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88262"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1819
                     /locus_tag="VFH83_03965"
     CDS             209..1819
                     /locus_tag="VFH83_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HET6485551.1"
                     /translation="MLSGMVNVPLELLFAAEKSHKDIYNQIEVDVLFRGPGEGTWKVP
                     AFWAGEGTFRVRFAPPKPGKYSYETACTDTADAGLHGRTGELEAVPYDGQNPLFRHGR
                     LQLARSKRYLEHADGTPFFWLGDTWWMGLCKRLSWPGGFRTLVADRVQKGFSLVQIIA
                     GLYPDMPAFDPRGANEVGFPWEEGYARIVPAYFDMADLRIDWLIRSGLVPCIVGCWGY
                     FIPWMGVEKMKKHWRNLVARWGAYPVVWCLAGEATMPYYLSTTKEEDAKVQKRAWTEL
                     GRYVRSIDPSHNPITIHPTNRGRDQVEDPSVLDVDMLQTGHGDRTSLPMTVKSVTEEY
                     ARTPTMPVIDGEVCYEGIGEASRQEVQRLMFWASILNGSAGHTYGANGIWQLNTREQP
                     YGPSPHGMSWGDTPWEDAYLLPGSGQLGLAKRLLERFEWWRFEPHPEWVEPHGTETNY
                     YAPYAAGIPGKVRVIYFPLGWESPVVKGIEKGTRYRAYYWNPVNGQQREIGEATADAK
                     GDWPVASGTEPWRLPPLYQDWVVVLQAV"
     gene            complement(1917..>2734)
                     /locus_tag="VFH83_03970"
     CDS             complement(1917..>2734)
                     /locus_tag="VFH83_03970"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET6485552.1"
                     /translation="ETYIERDGNAATARDFGETWKRKLNRDHFFYCMKNGYDLILGGA
                     DARVVGHWNIVTGSTVMCMEPVGTFHLCDPVNAAIDGTAISYMYQRGLDVVAASILAA
                     SVAAAFRPDATVDSVLRAALDAAPRTRMMTFDTRTVDTPYQYLSLCLEVADKYTDVLA
                     ARKELYERCLYYHMIDPLELLGLSYAMFKIAKGDMRLCAIGGTNIGRDSDTISGRGAM
                     LAGTLRGSAGIPKEWIAMFRPESLERIRTNAGRIASLIEHRKLPLLKERQSIR"
BASE COUNT          484 a          868 c          936 g          446 t
ORIGIN      
        1 atcgagcgcg agctcgaccg ccggcttccc ccgctgctgg agcagggggg gttcatcccg
       61 cacatcgacc atgccatacc gccggacatc tcctacgaca acttccgcta ttacctggac
      121 cggaagatga agctgctggg attctgagcg ggaccggccg cgattctgaa aaagggacgg
      181 ccgggatttc cgagaagagg aggcacccat gctgagcgga atggtcaacg tacccctgga
      241 gctgctcttt gccgcggaga agagccacaa ggacatctac aaccagatcg aggtggatgt
      301 cctctttcgc ggccccggag aaggcacgtg gaaggtgccg gccttctggg ccggcgaagg
      361 gaccttccgt gtccgctttg ccccgcccaa gccggggaag tactcgtacg agactgcgtg
      421 cacggatacg gccgacgcgg gtctgcacgg gcgcacagga gagctggagg cggtccccta
      481 cgacggacag aacccgctgt tccgtcacgg gcggctgcag cttgccagga gcaagcgcta
      541 cctggagcac gccgacggca cgcctttctt ctggctgggg gacacctggt ggatggggtt
      601 gtgcaagagg ctttcctggc ccggcggttt ccgcaccctg gtggcggacc gggtgcagaa
      661 gggattctcg ctcgtccaga tcatcgcggg tctgtacccc gacatgccgg ccttcgatcc
      721 gcgaggcgcc aacgaggtgg ggttcccctg ggaagaaggg tatgccagga tcgtgccagc
      781 ctatttcgac atggcggacc tcaggatcga ctggctgatc cggtccgggt tggtaccctg
      841 catcgtcggc tgctggggct acttcattcc gtggatgggc gtggagaaga tgaagaagca
      901 ctggcggaac ctggtggccc ggtggggcgc ctacccggtc gtgtggtgtc tggccggaga
      961 ggccaccatg ccgtactacc tctccactac gaaggaagaa gacgccaagg tgcagaagcg
     1021 ggcatggacc gagctggggc gctacgttcg gagcatcgat ccgagccaca accccatcac
     1081 gatccatcct accaaccgcg ggagagatca ggtggaggat ccgtcggtcc tggacgtaga
     1141 catgctccag accggtcatg gcgatcggac cagcctgccc atgacggtga agtccgtgac
     1201 ggaggagtac gcgcgcaccc cgacgatgcc cgtcatcgac ggcgaggtct gctacgaagg
     1261 gatcggcgag gccagccggc aggaggtcca gcggctcatg ttctgggcaa gcatcctgaa
     1321 cggctccgca gggcacacct acggcgccaa cggcatctgg cagctcaaca cgcgggagca
     1381 gccctacggg ccctccccgc acggcatgag ctggggcgac acgccctggg aggacgcgta
     1441 cctgctgccc ggctcgggcc agctcgggct ggcaaagagg ctgctggagc gcttcgagtg
     1501 gtggcggttc gagccgcacc ccgagtgggt cgagccgcac ggcacggaga cgaactacta
     1561 cgcgccgtat gccgcgggga tccccggaaa ggtccgcgtc atctacttcc cgctgggctg
     1621 ggagagcccg gtggtgaagg ggatcgagaa ggggacgcgg taccgcgcct actactggaa
     1681 ccccgtgaac ggacagcagc gtgagatcgg tgaggctacg gcggacgcca agggagactg
     1741 gccggtagcc tccggcacgg agccctggcg cctgccgccg ctgtaccagg actgggtggt
     1801 ggtcctacag gcggtctagc gtccgcccaa ggggcggtct agcgtccgcc caaggggcgg
     1861 tctagctcgg gaaggtcggg ccggggcggt ggagccgtcc cccggcccga cagggtctat
     1921 cggatggatt gccgctcctt caggaggggc agcttccggt gctcgatcag agaggcaatc
     1981 cgccccgcgt tcgttcggat ccgttcaagg gactccggcc ggaacatcgc aatccattcc
     2041 ttgggaattc ccgcggatcc ccgaagcgtc ccggccagca tcgcgccccg ccccgagatg
     2101 gtgtcggaat cccggccgat gttcgtgcct ccgatggcgc acagcctcat gtcccccttg
     2161 gcgatcttga acatggcgta ggagagaccc aggagctcga gcgggtcgat catgtggtag
     2221 tacaggcacc gctcgtacag ctcctttcgg gcggccagga cgtccgtgta cttgtccgcc
     2281 acctccaggc aaagcgaaag atactggtac ggcgtatcca ccgtccgcgt gtcgaacgtc
     2341 atcatgcgcg tgcgcggcgc ggcatccagg gcggcgcgca ggacgctgtc cacggtggcg
     2401 tccggccgga acgccgccgc cacggaggca gccaggatgg aggcagccac cacgtccaga
     2461 ccgcgctggt acatgtagct gatggccgtg ccgtcgatgg cagcgttgac cggatcacag
     2521 aggtggaagg tgccgaccgg ttccatgcac atcacggtgg acccggtcac gatgttccag
     2581 tgccccacca cgcgcgcatc ggcgccgccc aggatcaggt cgtacccgtt cttcatgcag
     2641 tagaagaagt ggtcccggtt cagcttcctc ttccaggtct ctccgaagtc ccgggccgtt
     2701 gccgcgttcc cgtcccgctc gatgtaggtc tccg
//
LOCUS       DASRZS010000160         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3936082, whole genome shotgun sequence.
ACCESSION   DASRZS010000160 DASRZS010000000
VERSION     DASRZS010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3936082"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFH83_03975"
     CDS             complement(<1..185)
                     /locus_tag="VFH83_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6485553.1"
                     /translation="MAERYRVLVVGMGKRGVHHAAAFHANPRFQVAGLADVDPGKLKD
                     AARFGAPQTGTDPGSLAR"
     gene            complement(233..1357)
                     /locus_tag="VFH83_03980"
     CDS             complement(233..1357)
                     /locus_tag="VFH83_03980"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6485554.1"
                     /translation="MKVGFYGHVRQYHAIKSEIDTRLQEVLESGEYVMGPMLKRFEKE
                     LCDYTGLKYAVGVNSGTDAIWLSLLALGIGPGDECITTANTFFATAEAVWFTGATVVF
                     VDSDPRTNCIDPNLIEKAITRKTRAIIPVHLYGQCADMKAIRAIADRHSLRVIEDNAQ
                     GFGARGNGFRIGELGDAVCTSFIIQKNLGTFGDGGAVVTNNPEIDRIARKLRNHGSDK
                     RSVHSMGYNSRLDDIHAGFLSVKLKHIDAWNAQRRTWSERMTRGLSGARTFTLPYAAP
                     GYYHIYHLYVIETKKPEHRDQLLKFLNDNGIDAKCHYPIAIHQQEGYPWGKQARIVGP
                     LTQSERNAATCVSLPMYPEMTAEEVDFVAAKTMEWDAAHA"
     gene            complement(1454..1526)
                     /locus_tag="VFH83_03985"
     tRNA            complement(1454..1526)
                     /locus_tag="VFH83_03985"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1491..1493),aa:Met,seq:cat)
     gene            1682..>2107
                     /locus_tag="VFH83_03990"
     CDS             1682..>2107
                     /locus_tag="VFH83_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485555.1"
                     /translation="MAERARFLLLACLCVAVTATSAVYFVRWMLKPNTGLVVDYPQVI
                     VGNGRAIFSPRVPFSAAVSSGLLPDRDRLIRVNGAPVASSLDLLRADATIRSYSGFPV
                     VVERPEIGPVSLTVTPWFTPARADWVFVLAFCLALACVAF"
BASE COUNT          312 a          700 c          676 g          419 t
ORIGIN      
        1 cgcgcgaggg acccagggtc cgttccggtc tggggggcgc cgaaccgggc ggcgtccttc
       61 agcttgcccg gatcaacgtc cgcgagcccc gcgacctgga accgcggatt ggcgtggaac
      121 gccgcggcat ggtgcacgcc gcgcttgccc attccgacca ccaggactcg gtatcgctcg
      181 gccatgatcg tcctccgtca cgagtgtcaa cgacgcgcgg cgcgggagag agctatgcgt
      241 gcgccgcgtc ccactccatg gtcttggcgg ccacgaagtc cacctcctcc gcggtcatct
      301 cgggatacat gggcagggag acgcacgtcg ccgcgttccg ttcggactgc gtgagaggac
      361 cgacgatgcg ggcctgcttt ccccacggat acccttcctg ctggtggatt gcgatagggt
      421 agtggcactt ggcgtcgatc ccgttgtcgt tcaggaactt caggagctga tcccgatgct
      481 ccggcttctt cgtctcgatc acgtacaggt ggtagatatg gtagtacccc ggcgccgcgt
      541 acggcagcgt gaacgtccgc gctccgctca gcccgcgggt catccgctcg gaccacgttc
      601 gccgctgggc gttccaggcg tcgatgtgct tgagcttcac gctcaggaac ccggcgtgga
      661 tgtcgtccag ccggctgttg tagcccatgc tgtggacgga ccgcttgtcg gagccgtggt
      721 tgcggagctt ccgggcgatc cgatcgatct cggggttgtt cgtgaccacg gcgccgccat
      781 ccccgaacgt gccgagattc ttctggatga tgaagctcgt gcacaccgca tcgcccagct
      841 ctccgatgcg gaacccgttg ccccgggctc caaatccctg ggcgttgtcc tcgatgaccc
      901 gaagggaatg ccggtccgcg atggcccgga tggccttcat gtccgcgcac tggccgtaca
      961 ggtgcacggg gatgatggcc cgggtcttcc gggtgatcgc tttctcgatg aggttcgggt
     1021 cgatgcagtt ggtccgcgga tcgctgtcca cgaagacgac ggtggccccg gtgaaccaga
     1081 ctgcctccgc tgtggcgaag aacgtgttcg ccgtagtgat gcactcgtcc cccggtccga
     1141 tccccagagc cagcaaggaa agccagatgg cgtcggtacc gctgttgacc cccaccgcgt
     1201 acttcagtcc ggtgtagtcg cagagctcct tctcgaagcg cttgagcatc ggacccatga
     1261 cgtactcacc gctctccagc acttcctgga ggcgcgtgtc gatctcggac ttgatggcgt
     1321 ggtactggcg aacgtgaccg tagaagccta ccttcatggg agcctcctca acgacgtttc
     1381 agatcgatgc tgccgatcgc gaatcagggt cgaaggccag ccctgcgact ggcctcaccc
     1441 ggaagcggga gaatagcggc gaggggactc gaacccttga cactgcggat atgagccgca
     1501 tgctctacca tctgagctac gccgccgttt tgcgagcgcg ggcactatac cagcggcccg
     1561 agtgggtgtc aactccggcg ctctcggctt gacggcttcc tcccggcgaa ctagaatcgc
     1621 ggaccagggc gcgacctgga cccgctccag ccgcgctgat tcaaaatgga gggttgatcc
     1681 cgtggctgag cgagcccgct ttctcctgct ggcatgtctc tgcgtcgcgg tcaccgcgac
     1741 ctccgcggtc tacttcgtcc ggtggatgct gaagccgaac accgggctcg tggtggatta
     1801 cccccaggtc atcgtgggca acggtcgggc gatcttctcg ccgcgggttc ccttctccgc
     1861 ggcggtgtcg tccggcctgc tccccgaccg ggaccggctc atccgcgtga acggagctcc
     1921 cgttgcctcc agtctcgacc tcctcagggc cgacgccacg atccgcagct actccgggtt
     1981 tcccgtcgtg gtcgagagac cggagatcgg tccggtttcg ctgacggtga ccccctggtt
     2041 caccccggcc agggccgatt gggtcttcgt cctggcgttc tgtcttgccc ttgcgtgcgt
     2101 ggcgttc
//
LOCUS       DASRZS010000161         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_861382, whole genome shotgun sequence.
ACCESSION   DASRZS010000161 DASRZS010000000
VERSION     DASRZS010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861382"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VFH83_03995"
     CDS             <1..878
                     /locus_tag="VFH83_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6485556.1"
                     /translation="SCREKAIAVCQGLAEVSMCLPYMVEELEGTLATLQKSHQVAQDR
                     LVQTERLASMGQISAGVAHEINNPLSTILLYSHMLLKAHRDGDAESEDIQMIVNEANR
                     CRYIMRGLLDFARQTRVVKAPADLGTLARDVVDGMVMRLGESPTRVRCEVQEGMPAAS
                     VDGEQVRQMLVNLVQNGIDAVESTGRPGEVHVSVGLSESADLAVLRVRDTGCGMPAEV
                     VKEIFTPFYTTKALGKGTGMGLSIVYGVVKMHAGDISVDSQVGAGTTFTVRIPIGARS
                     REDSHGLGQDSAAGR"
     gene            844..1233
                     /locus_tag="VFH83_04000"
     CDS             844..1233
                     /locus_tag="VFH83_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6485557.1"
                     /translation="MGSDKTVLLVDDDADFVEMNRTLLRENGYEVRVAYSGRQCREEV
                     SARPPDLIILDMVMESSSVGFDLSRDLRNSERTKAIPLVMITSVNDSNPFRLEPDRTW
                     LPVDALLEKPVDPNLLLAVVDRALKGP"
     gene            1262..1657
                     /locus_tag="VFH83_04005"
     CDS             1262..1657
                     /locus_tag="VFH83_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6485558.1"
                     /translation="MSALIYLVDDDRDFLDLLESILSRADYRVVAFSSPQSALAALDT
                     AAELPSILITDLMMKSLDSGFSLAAAIRADPRLRAVRLIIVSAVASQRGFDFHPRSAQ
                     DLAAMHADAFFDKPVVPRALLDKVEELLR"
     gene            1654..>2777
                     /locus_tag="VFH83_04010"
     CDS             1654..>2777
                     /locus_tag="VFH83_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6485559.1"
                     /translation="MTDAPSRILVVDDELGVREGCRKILASEGYDVVTAGDGKAGLEQ
                     FLDRGPFSAMLVDLQMPRMTGLELMREVRGRDEEVVCIIITAHATIDTAVEGTRQGAY
                     SYIPKPFTPDELLLAVKNGLERRALSLEAKRLRHERERRLLELASERSRSNTIIACMT
                     DGILVINREKLVVLRNNAAARILPDCAQRELPFPLSEIASPDVREIISLVLDAPGGFM
                     ILSRELQLGPSTYMVNLSPIIEPDGETSGAVAMLSDVTALKKLETAKSMFVSMVAHEV
                     KSPLAATEGWLNLVLSGTLKDDRSEEKRVLGRALTRVKTLRSMVGELLSLTAIQTGNF
                     TLRREPLALGVVVTAAADAAREKAAERRIAIQVNPGPELRV"
BASE COUNT          469 a          912 c          945 g          451 t
ORIGIN      
        1 catcgtgccg ggagaaagcg atcgcggtct gccaggggct ggcggaggtg agcatgtgcc
       61 tgccgtacat ggtggaggag ctggagggca cgctggccac gctgcagaag tcccaccagg
      121 tggcgcagga ccggctggtg cagaccgagc ggctggcctc catgggacag atctccgccg
      181 gcgtggccca cgagatcaac aacccgctct ccacgatcct cctgtactcc cacatgcttc
      241 tcaaggcgca ccgcgacggg gacgcggaat ccgaagacat ccagatgatc gtgaacgagg
      301 ccaaccggtg ccgctacatc atgcgcgggc tcctggactt cgcccgacag acccgagtgg
      361 tcaaggcgcc ggcggacctg ggcacgctgg ctcgggacgt ggtggacggg atggtcatgc
      421 gtctgggcga gagcccgacg cgggttcgct gcgaggtgca ggaaggcatg cccgcggcga
      481 gcgtggacgg cgagcaggtc cgacagatgc tcgtcaacct ggttcagaac gggatcgacg
      541 ccgtggagag cacggggcgg ccgggagagg tccacgtgag cgtggggctc tccgaaagcg
      601 cggacctggc ggtgcttcgg gtccgggata ccggctgcgg catgcccgcg gaggtggtca
      661 aggagatctt cacgccgttc tacaccacca aggccctggg caaggggacc ggcatgggct
      721 tgtccattgt ctatggagtg gtgaaaatgc acgcgggaga catctccgtg gacagccagg
      781 tcggggccgg cacgaccttc acggtgcgca tccccatcgg cgctcgatcg agggaggata
      841 gccatgggct cggacaagac agtgctgctg gtcgatgacg atgccgactt cgtggagatg
      901 aaccggaccc tgctgcggga gaacggctac gaggtccgcg tggcgtacag cggccgccag
      961 tgccgcgagg aggtgagcgc acgccctccg gacctgatca tcctggacat ggtgatggaa
     1021 agctccagcg tggggttcga cctctcccgg gacctgcgca actccgagcg caccaaggcc
     1081 attcccctcg tcatgatcac ctccgtgaac gactccaacc ccttccgtct ggagcccgac
     1141 cggacgtggc ttccggtcga cgccctgctg gagaagccgg tcgacccgaa cctgctcctg
     1201 gccgtggtgg atcgggccct caagggaccc tgatgcgcgg ccctctgccg gagacgcccc
     1261 catgtccgcg ctgatctatc tcgtggatga cgatcgtgat ttcctcgacc tgctggagag
     1321 catccttagc cgggccgact accgagtggt ggccttcagc agcccccagt ccgcccttgc
     1381 cgctctggac acggcggcgg agctgccctc catcctcatc acggacctga tgatgaagtc
     1441 gctggactcc ggattctccc tggcggcggc catccgcgcg gatccccggc tccgcgcggt
     1501 gcgcctcatc attgtctccg cggttgccag ccagcggggg ttcgacttcc atccgcgctc
     1561 cgcgcaggac ctggcggcca tgcatgccga cgccttcttc gacaaacccg tggtgccgcg
     1621 agcgctcctg gacaaggtcg aggagcttct gcggtgacgg acgctccctc ccggatcctg
     1681 gtggtggacg acgagcttgg ggtccgggaa ggctgccgca agatcctggc atccgagggc
     1741 tacgacgtgg tgaccgccgg cgacggtaag gcggggctgg agcagttcct ggaccggggg
     1801 cccttctccg ccatgctggt ggaccttcag atgccgcgga tgaccggcct ggagctgatg
     1861 cgcgaagtgc gcggtcggga cgaggaggtg gtctgcatca tcatcaccgc ccacgccacc
     1921 atcgacaccg ccgtggaagg cacgcgccag ggcgcctaca gctacatccc caagcccttc
     1981 acgccggacg agctcctcct tgccgtcaag aacggcctgg agcggcgcgc cctgtccctc
     2041 gaggccaaac gtctgcgtca cgagcgcgag cgacgtctgt tggagctcgc ctcggagcgc
     2101 tctcgaagca acaccatcat tgcctgcatg accgacggga tcctggtcat caatcgcgag
     2161 aagctggtgg tgcttcggaa caacgccgcg gcgcggatcc tcccggattg cgcccagcgg
     2221 gagctcccgt ttcccctctc ggagatcgcc agccccgacg tgcgggagat catctccttg
     2281 gtgctcgatg cgccgggcgg tttcatgatc ctctctcgag agctccagct cggcccttcc
     2341 acctacatgg tcaacctcag ccccatcatc gagccggacg gcgagacctc gggcgccgtg
     2401 gccatgctga gcgacgtgac cgcgctgaag aagctggaaa ccgccaagtc gatgttcgtt
     2461 tcgatggtgg ctcacgaggt aaagagcccg ctggctgcca cggagggatg gctcaatctg
     2521 gtcctcagcg gcaccctgaa ggacgatcgg tccgaagaaa agcgcgtgct gggccgggcg
     2581 ctgacgcggg tgaagaccct gcgctcgatg gtgggggagc ttctgagcct caccgccatc
     2641 cagaccggca acttcacgct tcgccgggag cctctggcgc tgggggtcgt cgtgactgcc
     2701 gcggccgacg ccgcgcgaga gaaagcggcg gagcgcagga tcgccattca ggtcaacccg
     2761 gggcccgagc tgcgagt
//
LOCUS       DASRZS010000162         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4324664, whole genome shotgun sequence.
ACCESSION   DASRZS010000162 DASRZS010000000
VERSION     DASRZS010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4324664"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFH83_04015"
     CDS             complement(<1..438)
                     /locus_tag="VFH83_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485560.1"
                     /translation="MATLLVLSILAPLFWLVISSLASLKDLVARPLRWIPSTLGLERY
                     RDIFFGPVGLSFRNALRNSLVVAFLSTAASLVVAIVASYSLSRFPGRGRSSVLYVMLA
                     TYMMPPVAILLSLYITLRQLHLVNTVWGLSVVYCSFLIPFLTWI"
     gene            complement(492..1397)
                     /locus_tag="VFH83_04020"
     CDS             complement(492..1397)
                     /locus_tag="VFH83_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018153113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485561.1"
                     /translation="MRISSTGSARRTAAAWLYLIPATLLVAGIVGYPFLRTILNAFTD
                     APLISQSRPSAWVGIGNFLYAFRNASFQGGIVHTLYFCLLSITLNLVIGVLVALLLNQ
                     KFRGRAFVRTLVILPWALPTVVNAVLWRWIYNPSYGALNALLSQAHIIGEYQSWLGSP
                     GLGTMTMVVLADTWKNYGLVTILVLAGLQTIPVELYEASSVDGSTWWSNFTHITLPGI
                     QPAILIALVLKTIDGLRVFDIVYLMTAGGPASATKTLSFYVYQEAFGFLHLGQASTFA
                     VITVAIIAIFVYLYVRLIRSEGVNG"
     gene            complement(1384..>2512)
                     /locus_tag="VFH83_04025"
     CDS             complement(1384..>2512)
                     /locus_tag="VFH83_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET6485562.1"
                     /translation="NMTAEFQKLNPQVTVSPEYVPYEALHDKIVTAAASGSGYDIVLF
                     DVIWPAEFANQGILRDITNDIPKSMIDQVFAGAWESSRYKNHYYGVPWILDTQYLFYN
                     TEMLQKAGIKAPPKTLAELADQAKILKQKGIVQYPIIWAWQQSENVFLNYAQVFFGFG
                     GTWFDTKNNPTFNSGGGLKALEYMVQTIKDGTSNPASLQAIADDEVRIFGAGQAAFAI
                     NWTYMYNVANTDASQSTVVGKVGIVPAPGDGRNASIVPLNGSMGLGITQSCKNPREAL
                     SYISYLASAPVQAKYAALNLPMWKPYYDNPSQWPKGQDALMKAAKAAFTRLYDRPLLS
                     NYTQFSTIMQKYLQQALLLQVSPKEALDAAAKEVVQEGANQ"
BASE COUNT          482 a          745 c          851 g          434 t
ORIGIN      
        1 gatccacgtg aggaagggaa tgaggaacga gcagtagacg accgacaacc cccagacggt
       61 gttcacgagg tggagctgcc gcagggtgat gtacagggag aggaggatcg cgacgggagg
      121 catcatgtag gtcgcaagca tcacgtacaa caccgaggag cggccgcgcc cggggaaacg
      181 ggaaagcgaa tacgacgcga cgattgccac cacgaggctc gcggcggtgg acaggaacgc
      241 caccacgagg ctgttgcgga gcgcgttgcg gaaggacagg cccaccgggc cgaaaaagat
      301 gtcgcggtag cgctccaggc ccagggtgga cgggatccag cgaaggggcc ttgcgacaag
      361 gtctttcagg ctcgccaggc tcgagatgac cagccagaac agcggcgcga ggatgctgag
      421 gaccagcagc gtcgccatcg catagacgaa gatcgatctc gcgcgccggc gactccttcc
      481 ggggcggcgt ctcatccgtt gacgccctcg gaacggatca gccggacgta gagatagacg
      541 aagatggcga tgatcgccac ggtgatgacg gcgaaggtcg acgcctggcc gaggtggagg
      601 aagccgaatg cctcctggta gacgtagaac gacagggtct tcgtcgcgct cgccggtccc
      661 cccgccgtca tgaggtagac gatgtcaaag acgcgcagcc cgtcgatggt tttcaggacc
      721 agggcgatga ggatggcggg ctggataccc ggcagcgtga tgtgagtgaa attgctccac
      781 caggtggagc cgtccacgct ggacgcctcg tagagctcca ccgggatggt ctgcagaccg
      841 gcgagcacga ggatggtcac caagccgtag ttcttccacg tgtcggcaag caccaccatg
      901 gtcatggtac ccagccccgg gctccccagc cagctctggt actcgccgat gatgtgggcc
      961 tgcgagagga gcgcgttgag agcgccgtag gaggggttgt agatccagcg ccagagcacg
     1021 gcgttgacca ccgtgggcag ggcccacgga aggatcacca gcgtgcggac gaaggcccgt
     1081 ccgcggaact tctggttgag gaggagcgcg acgagcaccc cgatcaccag gttgagcgtg
     1141 attgaaagca ggcagaagta cagcgtgtgg acgatgccgc cctggaacga tgcgttgcgg
     1201 aacgcgtaga gaaagttacc gatgcccacc cacgcggagg gccgggattg cgagatgagg
     1261 ggggcatcgg tgaatgcgtt caggatcgtc cgcaggaaag gatatccgac gatccccgca
     1321 accaggagcg ttgcggggat caggtagagc catgccgccg ccgtccttcg cgcggagccg
     1381 gtgctactga ttcgcaccct cttggaccac ttccttcgcc gcggcatcca gcgcctcttt
     1441 cgggctcacc tggagcagca gcgcttgctg caggtacttc tgcatgatgg tcgagaactg
     1501 ggtgtagttg gagagcagcg gccggtcata cagccgcgta aatgccgcct tcgcagcctt
     1561 catgagagcg tcctgcccct tgggccactg ggacgggttg tcgtagtaag gcttccacat
     1621 cgggaggttc agcgcggcgt acttcgcctg gaccggcgcg ctggcgaggt agctgatgta
     1681 cgacagcgct tcccgcgggt tcttgcagga ctgcgtgatg cccagcccca tcgagccgtt
     1741 cagcgggacg atcgaggcgt tcctgccgtc gccgggggcg gggacaatgc ccaccttgcc
     1801 gaccacggtc gactgcgacg catccgtgtt cgccacgttg tacatgtagg tccagttgat
     1861 cgcgaaggcc gcctggcccg cgccgaagat ccgcacctcg tcatccgcga tggcctgcag
     1921 ggacgcgggg ttggacgtcc cgtccttgat ggtctggacc atgtactcca gggccttcag
     1981 accgcccccg ctgttgaacg tcgggttgtt cttcgtgtcg aaccatgtcc cgccgaaccc
     2041 gaagaacacc tgcgcgtaat tcaggaagac gttctccgac tgctgccacg cccagatgat
     2101 gggatactgg acgatgccct tctgcttgag gatcttcgcc tggtcggcca gctccgcgag
     2161 cgtcttcggc ggcgccttga tcccggcctt ctgcagcatc tcggtattgt agaacaggta
     2221 ctgcgtgtcg aggatccagg ggacaccgta gtagtggttc ttgtacctcg aggattccca
     2281 ggcacccgcg aagacctggt cgatcatcga cttcgggatg tcattggtga tgtcgcggag
     2341 gataccctgg ttggcgaact ccgcgggcca gatgacgtcg aagagcacga tgtcgtagcc
     2401 gctgccgctc gccgccgctg tcacgatctt gtcgtgcagc gcctcgtacg gcacgtactc
     2461 cggggacacc gtgacctggg ggttcagctt ctggaactcc gccgtcatat tg
//
LOCUS       DASRZS010000163         4146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4326044, whole genome shotgun sequence.
ACCESSION   DASRZS010000163 DASRZS010000000
VERSION     DASRZS010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4326044"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2842
                     /locus_tag="VFH83_04030"
     CDS             <1..2842
                     /locus_tag="VFH83_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6485563.1"
                     /translation="TTLQKMLNNLWMVLCLLLGSILAVALISSIPVYTEGVLQRLLTR
                     DLEDFQVKSSVFPGRSELSRTFVMPDEPDKKLPLFRFLDTEIERRLLPALGIPVLTST
                     RQLTLDYLTIYPEGRAPDPKADAKLTYVKLEGMRGFQDHMALVAGRAAAAGETDGTIE
                     AVISQKAMQDLGLYLDRTYDVWDAFYSTGRVARVRIVGVWTIRDPRDPYWFEGLGEYG
                     ESLMIDPALLEQRFIVDNSPNFSLATWHYAFDYHAIRIADVQALMARIQDYQRLASDN
                     RITWDFPMLDILKSYAVRASVLKTTLWFLQAPVLLMLVFFIYMVSQLILESDANEIAV
                     LKSRGASSPQIFARYLIEGTILAVLALAVGPLLGMSICLVLGSSNGFLEFVQRAALPL
                     GFSHGAALYAVLGALLFIAAMLLPALVASRTTIVEHKRKRGRARRGPVWKRFFVDGVL
                     LAVSGYGLYGYYTQQKILKLTGVTGGSLPLDPLLFVISVAFLLGAGMLFLRIYPSLVR
                     GIFWLGRKRWSPVLYTTFVHVGRAMGHEQFLMLFLILSLGLGVYNSVAARTVNRNVED
                     RVRYAVGADITLQPHWEDLAPMPSDRLTGPGGAVQGSDDTVTHQWVEPPFAPYQQIAG
                     TDLATKVYRNDQVTATFGTGQGVVTLMGIVPNEFGKVAWFRSDLLPVHQNAYLNLLGS
                     SPKAVLVSSNLRGPYNLRLGDPIYLTWAGQGLIDAYIYAFIDYWPTYNPLTRTNGRPA
                     SLVVANLAYLQAKMEMEPYQVWLRKSPGAGSAQVFDDIRQKGLQIESLSDSSQRVVAE
                     RNDPMLQGTNGALTLGFIIAMGISFVGFLLYWILSLKGRTLQFGILRAMGLRQSRVVL
                     MLILEQLLISGVAIAVGILVGTVASYLFVPLLQLTASAEAQVPPFAIVSLRSDFLKVL
                     ALAAAMLGSGAVLFRWMIRRIRIHQAIKLGEE"
     gene            2846..3544
                     /locus_tag="VFH83_04035"
     CDS             2846..3544
                     /locus_tag="VFH83_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485564.1"
                     /translation="MASAGGPRAILRAQGATREFRTGSTVVRALWDATLEIEQARLVL
                     LQGPSGSGKTTLINLLGALDHPTRGEIYFDGAPLHSLSEPRRDELRRSRMGFVFQSVG
                     LISLMSAYENVELGLRIAGYDARDRSARAERCLAFVGLARRMHHRPSELSGGEQQRVA
                     IARAIAHRPKVIFADEPTAELDTAMGLQVIARFKALVAEEGVTVVLSSHDPAIADFAD
                     TVIALEDGRVTRVG"
     gene            3618..>4146
                     /locus_tag="VFH83_04040"
     CDS             3618..>4146
                     /locus_tag="VFH83_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6485565.1"
                     /translation="MVCAENLVKIYKSSEIEVVALQGLDLTVKAGELMAIVGNSGSGK
                     STLLNIIGGLDLPSAGRIEVAGLDLTKFRDADFIRFRRETAGFVWQNVARNLVPYMSA
                     LQNVEVPMSIAGARDRRRRARELLDLVGLSHRMHARLGEMSGGEQQRAAIAIGMANTP
                     RLLLADEPTGNMDSVA"
BASE COUNT          656 a         1389 c         1372 g          729 t
ORIGIN      
        1 aacgacgctg cagaagatgc tcaacaatct ctggatggtg ctctgcctgc tcctgggctc
       61 catcctggcg gtcgccctga tctcgagcat ccccgtatac accgaaggag tgctccagag
      121 gctcctgacc cgggacctcg aggacttcca ggtgaagagc tccgtcttcc cggggcgctc
      181 cgagttgagc cggaccttcg tgatgccgga tgagccggac aagaagctcc ccctgttccg
      241 tttcctggac accgagatcg agcggaggct ccttccggcg ctcggcattc cggtcctgac
      301 gtcgacccgc cagctcacgc tggactacct cacgatctac cccgagggac gggcaccgga
      361 tcccaaggcg gatgcgaagc tgacgtacgt gaagctggaa ggcatgcgag ggttccagga
      421 tcacatggcg ctggtcgcgg gccgtgcggc cgcggcgggg gagaccgacg ggacgatcga
      481 ggcggtgatc tcccagaagg ccatgcagga cctggggctg tacctggacc ggacgtacga
      541 cgtctgggac gcgttctact ccaccggccg cgtggcgcgc gtgcggatcg tgggcgtatg
      601 gacgatccgg gacccgcggg atccctactg gttcgaggga ctgggggaat acggggagag
      661 cctcatgatc gatcccgcgc tgctggagca gcgctttatc gtcgacaact ctcccaactt
      721 cagccttgcc acctggcact acgcgttcga ctaccacgcc atccgcatcg ccgacgtcca
      781 ggctctcatg gctcggatcc aggactacca gaggctggcc tcggacaacc ggatcacctg
      841 ggacttcccc atgctggaca tcctgaagag ctacgcggtc cgggcctcgg tcctcaagac
      901 cacgctgtgg tttctccagg cgccggtcct tctcatgctc gtgttcttca tctacatggt
      961 gtcccagctc atcctcgaga gcgacgcgaa cgagatcgcc gtcctgaaga gccgtggcgc
     1021 cagctctccc cagatcttcg cgcgctacct catcgaggga acgatcctgg cggtgctggc
     1081 gctggcggtc ggaccgctcc tggggatgtc catctgcctg gtgctgggct catccaacgg
     1141 attcctcgag ttcgtgcagc gcgccgccct cccgctcggc ttctcccacg gggccgcgct
     1201 gtatgccgtc ctcggcgcgc tcctgttcat cgcggccatg ctcctgccgg cgctggttgc
     1261 ctcacggacc acgatcgtgg agcacaaacg caagcggggc cgcgcccgcc gggggccggt
     1321 ctggaaacgg ttcttcgtgg acggggtcct cctggccgtg tcgggttatg gtctgtacgg
     1381 ctactacacc cagcagaaga tcctgaagct cacgggcgtc accggaggca gcctgccgtt
     1441 ggacccgctc ttgttcgtga tctcggtggc cttccttctg ggtgcgggca tgctctttct
     1501 caggatctat ccgtccctgg tgcgcggcat cttctggctg gggagaaaac ggtggagccc
     1561 cgtcctctac accaccttcg ttcacgtggg cagggccatg ggccacgagc agttcctgat
     1621 gttgttcctg atcctctccc tgggtctggg ggtgtacaac tccgtggccg cgcggacggt
     1681 gaaccgaaac gtggaggacc gggtccgcta cgcggtcggc gccgacatca cgctgcagcc
     1741 gcactgggaa gacctggccc ccatgccctc ggaccggctc acggggccgg gaggcgcggt
     1801 ccagggctct gacgacacgg tcacccacca gtgggttgag ccgcccttcg ccccgtacca
     1861 gcagatcgcc gggaccgatc tggccaccaa ggtctatcgg aacgaccagg tgaccgccac
     1921 gttcggcacg gggcaggggg tggtcaccct gatggggatc gtccccaacg agttcggcaa
     1981 ggtagcgtgg tttcggtcgg atctcctgcc ggtccatcag aacgcgtatc tcaatctgct
     2041 ggggagcagc cccaaggcgg tgctggtctc gtccaatctc cgcggcccgt acaacctgcg
     2101 cctgggggat cccatctacc tgacctgggc cggtcagggc ctgatcgacg cgtacatcta
     2161 cgccttcatc gactactggc ccacgtacaa cccgctgacc cggaccaacg ggcggcccgc
     2221 cagcctcgta gtggcgaacc tggcttacct ccaggccaag atggaaatgg agccttacca
     2281 ggtgtggctg cggaagagcc cgggggcggg cagcgcgcag gtcttcgacg acatccgaca
     2341 gaagggactc cagatcgaga gcctgagcga ttcgtcccaa cgcgtcgtgg ccgagcgcaa
     2401 cgaccccatg ctccagggca ccaacggagc gctcaccctg ggattcatca tcgccatggg
     2461 gatcagcttc gtggggttcc tcctgtactg gatcctctcg ctgaaaggtc ggaccctcca
     2521 gttcggcatc ctgcgggcca tggggcttcg ccagagccgc gtcgtgctca tgctgatcct
     2581 ggagcagctc ctgatctccg gcgtcgccat cgccgtcggc atcctcgtag ggaccgtggc
     2641 aagctacctt ttcgtgccac tcctccagct caccgccagc gccgaggccc aggtcccacc
     2701 gttcgccatc gtctcgctgc gatcggattt cctgaaggtc ctggcgctgg cggcggccat
     2761 gctcgggtcc ggtgccgtgc tcttccgctg gatgatccgg cgcatccgga tccatcaggc
     2821 catcaagctg ggggaggagt aggccgtggc atccgccggc ggtccgcggg cgatccttcg
     2881 ggcccagggc gcaacccggg agttccggac cggttcgacg gtcgtgcggg cgctctggga
     2941 tgcgacgctg gagatcgagc aggcccggct cgtcctcctc caggggccgt ccggctccgg
     3001 caagaccacg ctgatcaacc tcctgggtgc cctggaccac ccgacgcggg gcgagatcta
     3061 cttcgatgga gctccgctgc actccctgag cgagccgcgg cgggacgagc ttcgccgaag
     3121 ccggatgggg ttcgtgttcc aatccgtggg tctcatctcc ctcatgtccg catacgagaa
     3181 cgtcgagctg ggtctgagga tcgccggcta cgacgcgcgg gatcgaagcg cgcgcgccga
     3241 gcgatgcctg gccttcgtgg gtcttgcccg tcgcatgcat catcgtccca gcgagctctc
     3301 gggaggagag cagcagcgcg tggccatcgc ccgagccatc gcgcaccgcc ccaaggtcat
     3361 cttcgcggac gagccgacgg cggagctgga cacggccatg gggctgcagg tgatcgctcg
     3421 gttcaaagcg ctggtggcgg aagagggcgt gaccgtggtc ctctccagtc acgatccggc
     3481 catcgccgat tttgccgaca ccgtgatagc cctggaagac gggagggtga cccgtgtcgg
     3541 atgagatgga cccgcgtccg ccgggagggg cccggccgcg gggcgcccac ccgcccgccg
     3601 cgcccgtggc ggacaccatg gtctgcgcgg agaacctcgt caagatctac aagtcctcgg
     3661 agatcgaggt ggtggcgctc caaggtctgg atctcaccgt gaaggccgga gagctcatgg
     3721 cgatcgtcgg gaactccggc agcggaaaga gcacccttct gaacatcatc ggagggctgg
     3781 acctgccttc ggcggggcgg atcgaggtgg ccgggctgga cctgacgaag ttccgggacg
     3841 cggacttcat ccggttccgc cgggagaccg ctgggttcgt ctggcagaac gtggcccgaa
     3901 acctcgtccc gtacatgagc gccctgcaga acgtggaggt gcccatgagc atcgccggcg
     3961 ctcgggaccg ccgccggcgc gccagagagc tgctggatct ggtcgggctc tcccaccgca
     4021 tgcacgcgcg tctcggggag atgtccggcg gggagcagca gcgcgccgcc atcgccatcg
     4081 ggatggcgaa cactccgagg ctcttgctgg cggacgagcc cacgggcaac atggactccg
     4141 tggcgg
//
LOCUS       DASRZS010000164         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3172656, whole genome shotgun sequence.
ACCESSION   DASRZS010000164 DASRZS010000000
VERSION     DASRZS010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3172656"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            483..2792
                     /locus_tag="VFH83_04045"
     CDS             483..2792
                     /locus_tag="VFH83_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6485566.1"
                     /translation="MLLDHQVAPSPLAAGEGPTETRSAEPLLLPAGRQYTTLRVVLIK
                     PSKYDDEGYVIRFWKGVLPSNTLNVLHGLTADIARRRVFEDVLIQVDTFDETVEKVPV
                     RKIVAWASRPATKVVVCLVGVQTNQFPRALDLGREFRAHGLDVLMGGFHTSGTINMFS
                     EQAPDIQRLFLESIVVVSGEVEGYWEGILADVLNGALKPLYSFAQDLQNLVDIAQAPL
                     PVVSPRTMRHFALASFSTVDTSRGCPFTCSFCSIINVQGRTMRERSTEAIVEFVRTNY
                     RQHGIQFYFFTDDNFARKKQWRETFEGLIRLRTEEGVPVSFMMQVDLAKKPQDFVRLA
                     AAAGCSQVFMGVESVNPQNLQVASKRQNKVAAYQDSIREWHEAGIVVHAAYIIGLPFD
                     TAEQVPRDVQHLMEAIQPDQASFFMLTPIPGSQDHVEMTQRGERMDPDLNKRDSFHAT
                     IRHPHMSPAAWTAAYRDAWKIFYSKEHLIQILSRWKHRPGAYWNLFFVYCWYKNAALI
                     EHEHPMIAGFLRLKDRCIRRPGCAIDAWSVHVWKRTRELLGLIRSWVGLLKEMEEVWL
                     ATRPRSDREVQFFAEIERIQGEVWRALRIPEWQRMYTEAKDGLPAKARALLDPFEELT
                     SVVLLTPEDLDRFLQRWGNLHTRTRALYRRVAGEQGSARRWLDALHRLALEVKDSPKV
                     PEWRQAYARVRASLPSSLPRMSVGFDAANARVVYSRQHLRRVWSRTWEHLRTGQVWRI
                     QPGRLAMTALQEVQLATSFSQQVLSKPQH"
BASE COUNT          599 a         1056 c         1046 g          581 t
ORIGIN      
        1 cagaaaagca ccgggggggc tacggtgcct gccgctccat ctcattcctt ttgaatcccg
       61 gataccacgt ccggaatcaa actagactaa tacagctgga tcgaatgcgg cgatgcagcc
      121 ttggggtggt tccggtggga atcccctgag cattcggcga cgctcagacc ggccggaaag
      181 accccattcc cacgaaccaa gttccgaaat ctcggagttg tcctatccgg gcacccatgg
      241 gctgtcagtg cccaggttta aagcaagcta tcggatttaa tacgatcggg ctggtgcgcg
      301 gcccccgaag attggtgtgg atatatcttt gcatgaactc ccggtgatga acggatgcac
      361 tcacagggaa ctgtgcatct cgcggcgctg aggattgtac gatgcgccgc ccgagcaggc
      421 ggcgatgggc gaaaggcggc gtcggccgtc acggcatgaa gcgccgcgtc agagaggtcc
      481 gcatgttgct tgaccaccag gttgccccgt ccccacttgc cgccggtgag gggcccactg
      541 agacgcgctc ggccgagcct ctgctgctgc cggccgggcg ccagtacacg accctccggg
      601 tggtgctgat caagccatcc aagtacgacg atgaagggta cgtcatccgg ttctggaaag
      661 gggtcctccc gagcaacacc ctgaacgtgc tgcacgggct gaccgcggac atcgcccgac
      721 gccgcgtctt cgaggacgtc ctgatccagg tcgatacctt cgatgagacc gtggagaagg
      781 tgccagtccg gaagatcgtg gcctgggcca gccggcccgc gaccaaggtg gtggtatgcc
      841 tggtcggggt gcagacgaat cagttccccc gggcgctgga cctgggtcgc gagtttcgcg
      901 cgcacggcct cgatgtcttg atggggggct tccacactag cgggaccatc aacatgttct
      961 ccgagcaggc accggacatc cagaggttgt ttctggagtc gatcgtcgtc gtgtcgggcg
     1021 aggtggaggg gtactgggaa ggcatcctcg ccgacgtact gaacggcgcg ctcaagccgc
     1081 tctactcctt cgcccaggac ctccagaatc tggtggacat cgcacaggcg ccgctcccgg
     1141 tggtgagccc ccggacgatg cggcacttcg ccctggcgtc ctttagcacg gtggatacgt
     1201 cgcgggggtg cccctttacc tgcagcttct gctccatcat caatgtgcaa ggccgcacca
     1261 tgcgggaacg gtccaccgag gccatcgtgg agttcgtccg gaccaactac cggcagcacg
     1321 gcatccagtt ctacttcttc acggacgaca acttcgcccg gaagaagcag tggcgcgaaa
     1381 ccttcgaggg actcatccgc ctccggaccg aggaaggcgt cccggtcagc ttcatgatgc
     1441 aggtggacct ggccaagaag ccgcaggact tcgtgcgtct ggcggcagcc gccggctgct
     1501 cgcaggtctt catgggcgtg gagagcgtga atccccagaa cctgcaggtg gcgagcaagc
     1561 ggcaaaacaa ggtcgcggcc taccaggaca gcatccgcga atggcacgag gccgggatcg
     1621 tcgtccacgc cgcctacatc atcggcctcc ccttcgatac cgcagagcag gtgccccgcg
     1681 atgtccagca cctcatggaa gcgatccagc cggatcaggc ctccttcttc atgctcaccc
     1741 cgatccccgg gtcccaggat cacgtagaga tgacgcagcg cggcgaacgg atggacccgg
     1801 acttgaacaa gcgggactcc ttccatgcga ccatccggca tccgcatatg agcccggcgg
     1861 cgtggaccgc cgcctaccgc gacgcctgga agatcttcta tagcaaggag cacctcattc
     1921 aaatattgag ccgctggaaa caccgtccag gcgcctactg gaatctcttc tttgtgtatt
     1981 gctggtacaa gaacgcggca ctgatcgagc acgagcaccc gatgatcgcg ggcttcctac
     2041 gcctcaagga tcggtgcatc cgccgtcccg ggtgcgccat cgacgcgtgg tcggtgcacg
     2101 tctggaagcg gacccgggag ctgctcggcc tgatccgctc ctgggtcggg ttgctgaagg
     2161 agatggaaga ggtctggctc gccacccgcc cccgcagcga ccgggaggtg cagttcttcg
     2221 cggagatcga gcgcatccag ggcgaggtgt ggcgggcgtt gagaatcccc gagtggcaga
     2281 ggatgtatac cgaggcaaag gacgggctcc ctgccaaagc ccgcgcgttg ctcgatccgt
     2341 tcgaagaact gaccagcgtg gtcctgctca cgccagaaga cctcgaccgg ttcctgcagc
     2401 gatgggggaa tctgcacacc cggacgcgcg cactttaccg tcgggtggcc ggggaacagg
     2461 gatcggcgcg gcggtggctg gacgcgctgc accgcctggc cctggaggtg aaggactcac
     2521 ccaaggtacc cgagtggcga caggcttacg cccgcgtgcg tgcctcgctc ccgtcgagcc
     2581 ttccacggat gtccgtcgga ttcgatgcgg ccaacgcccg tgtcgtgtac tcgcgccagc
     2641 atctcaggcg ggtgtggagt cgcacctggg agcatctccg gacgggccag gtctggagga
     2701 tccagccggg ccgcctcgcg atgaccgccc tccaggaggt tcagctagcg acctcgtttt
     2761 cccagcaggt gctatcgaag cctcagcact gaatgggtcg tacacagtgg gcgtcctaca
     2821 ggccgaatcc tcccatctgt gcacccccgg tctcgaaggc cttccgcgca gggggccggg
     2881 tggcgtcctc caccgggggc gccgtccccc gttctgtcag cggatttctg aggcaggaca
     2941 ctcgggttcc tcctgacgaa agacaacgtg ccttttgccc agcccgagaa cgtcgacagc
     3001 ctggaaggag gcggtgagag actggtgagg agcacccaca ttccacgccg ccctccggcc
     3061 cgctcgagtt gatcccgtcc tcgcgccgac cgccccagcg ttatgcatct gagtacgatg
     3121 cggcggcgcc agtccttgac gaatctccga ttggtcgggg caactccgaa atctcgggcg
     3181 ttttccgatg acatgctgcc gagattccac ggccgcatcg ccccaactgc ctgattcaac
     3241 cgcgttgagg tcgcgtcgag ccgccagcag ctcgggttgg at
//
LOCUS       DASRZS010000165         6504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4326810, whole genome shotgun sequence.
ACCESSION   DASRZS010000165 DASRZS010000000
VERSION     DASRZS010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4326810"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFH83_04050"
     CDS             complement(<1..415)
                     /locus_tag="VFH83_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014651200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485567.1"
                     /translation="MRRERGTLREGLQSAAGTRLFLMALPFLAVVFLFSYLPLYGWVY
                     AFFDYRAGLRLRDVRFTGLKYFAALTSNPISSREILRVLRNTFALSLLSILTSPLPVV
                     FAIFLLEIRAQWYRRIVQTLTTLPNFISWILVYSVA"
     gene            519..2111
                     /locus_tag="VFH83_04055"
     CDS             519..2111
                     /locus_tag="VFH83_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6485568.1"
                     /translation="MYRALIVDDEPVVLRGLEKLIPWEEYGVEVGGRATTASKAMEQL
                     SGSAPFDILITDVRMPGMSGLDLIGRARELQPRLCCIVISAHDEFEYARAAIRLGAEN
                     YLLKPISRQELSSTIERTVEKLERERSASTPREDQPAAFRENILTRWLSGTITQQELA
                     ERAGLAGLNPYALEYAVLAAAVLEHPGLEKPEAVSALQRALLPRLAEQGPSDCIVDSS
                     GRLLILIQGSELSARTALVRAAVQEGLKRVHQERGIHAFASLGAVVRGAQRVEVSCRS
                     ALLLQGYRMFCPADSVVSADEVPSSAGPRAGWDFAEIEELIRDGREEQARAYLRGVLS
                     WFRADDGPPLAERKAALTDLILHIVAPVRQAAGGPQGMPASLANLFVSMEEAQTEERL
                     EAWLCSVLETAMSSVRSQSDGTTPIARHLMDYIREHYDQEISLKVFAAENHVNASYLG
                     QLFKRETGMMFTEYLNRIRMRRSEEMIRTEGLRIGEIARRVGFAHVSYFNHAFRKAFG
                     SSPLEYRRMAAGGPPQRPRSDR"
     gene            complement(2313..2659)
                     /locus_tag="VFH83_04060"
                     /pseudo
     CDS             complement(2313..2659)
                     /locus_tag="VFH83_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009592812.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(2720..3208)
                     /locus_tag="VFH83_04065"
     CDS             complement(2720..3208)
                     /locus_tag="VFH83_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6485569.1"
                     /translation="MNEIIYGLGGKPVLFMGQSRYFWGIVVGSDLWKELGWNAIIYLA
                     AIAAIDPELHEAATVDGAGRWRQIIHVTLPGINTIVIVLLILSIGNLITIGFEKRFQL
                     MNPVVHDAAQVLDLYALKYGLQSNGFGRGASINVVNSLVSLGLLFTANGLFRRKTGQS
                     VM"
     gene            3250..3498
                     /locus_tag="VFH83_04070"
     CDS             3250..3498
                     /locus_tag="VFH83_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485570.1"
                     /translation="MGRADFGQLGTGATADVLTPTQILAGGVVAIAGGLVHTAIIKAD
                     HSIWTTGYSSNGELGNGSSSGSLTFVQIRRPSLAPGDP"
     gene            complement(3500..4744)
                     /locus_tag="VFH83_04075"
     CDS             complement(3500..4744)
                     /locus_tag="VFH83_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET6485571.1"
                     /translation="MDSAFLHSYARVLLQVGVNLQPGQNLRILAEPVQREFMLIVAEE
                     AYRLGGRFVRLEYADARLTRIQAQTLESRHLDYVPGFYAKDLQTYLDEEWSLLRLEGQ
                     EDPDILADVDKARLATMRQASSRAGHFFQQQVMTNRLTWCVAPAPTPRWARKVLGDAG
                     GSDPAGVDPVEGLWKLFVPILRLDHSDPVAAWKEHSEHLQRRGRALNAMRLEALRFEG
                     PGTDLTVGLMPRSRFIGGGAVSQKGITFIPNLPTEEVFTTPDARRTEGTLRCTRPVEV
                     LGVPVEGITMEFREGLVRRCDAVRGRDALERYLTIDPNAGRAGEISLVDISSPVHRSG
                     RVYHSILFDENAACHLALGNGYPDAIEGGEKLSEDELRALGCNVSLVHTDFMIGSPEV
                     DVFGVTAGGRRTAILRDGSFVI"
     gene            complement(5195..6376)
                     /locus_tag="VFH83_04080"
     CDS             complement(5195..6376)
                     /locus_tag="VFH83_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET6485572.1"
                     /translation="MNIERLLAPRTRLMGASAIREILKVVSQPGIVSLAGGIPAPESF
                     PMELMGTLAERVLSRYGSRSLQYDRTEGFEPLQEALAARLALRGIAASARSVLVTSGS
                     QGTLDAVGKLLIAPGDAVAVEAPTYLGALQAWNPYEPRYLTVETDDDGAVPESLDRVL
                     SRERVKFVYLVPTFQNPTGRTLPLDRRRSIAQILQRHEALLVEDDPYSDLRYRGTPLP
                     PIQSLAPDNVVYTSTLSKTLAPGLRIGFAIAPAPLDRWLVIVKQGVDLHTSTFGQAMA
                     AEYLSGGHLDRHLPRILDLYRPRQEAMLSALARHLEHGFHWSRPEGGMFIWVEGPAGL
                     DTVAMAPACIRRGVAYVPGTFFYPGEGQGLATMRLNYTMADAATIESAIKTLSEVLRL
                     D"
BASE COUNT         1213 a         2060 c         2187 g         1044 t
ORIGIN      
        1 acgccacgga atagaccagg atccaactta tgaagttcgg cagggtggtc agcgtctgca
       61 cgatgcgccg ataccactgc gcccggatct ccagcaggaa gattgcgaac acgacgggaa
      121 gcggcgaggt gaggatgctc aggaggctca gggcgaaggt gtttcgcagc acccggagga
      181 tctcgcggga ggagatggga ttgctggtga gggccgcgaa gtacttcagg cccgtgaagc
      241 gcacgtcccg aagcctcagg ccggcccggt agtcgaaaaa ggcgtagacc cagccgtaca
      301 gcggcaggta ggaaaagagg aagaccacgg ccaggaacgg gagagccatc aagaagagcc
      361 tggtgccggc cgcgctctga agcccctcgc gcagtgtgcc tcgctcacgc ctcatggtgc
      421 tgcgcgccag tatggcgcgc cggatccttc ggcgaaatag aaataatcct ggaaacttgc
      481 gataatcgtg gaaatcgctc gggaaaggag cctgcgcggt gtatcgagcg ctgatcgtag
      541 acgatgagcc cgtcgtgctg cggggcctgg agaagctcat cccctgggag gaatacggcg
      601 tggaggtcgg cggccgcgct accaccgcct caaaagcgat ggagcagctc tcggggagcg
      661 cgccgttcga cattctgatc accgatgtgc gcatgccggg catgagcggc cttgatctga
      721 tcggcagggc cagggagctt cagcctcgcc tgtgctgcat cgtcatcagc gcccacgacg
      781 agtttgagta cgcccgagcc gccatccgat tgggggcgga gaactacctt ctgaaaccga
      841 tcagccgcca ggagctctcg agcaccatcg agaggaccgt cgagaagctg gagcgggagc
      901 ggtccgcctc gacccccagg gaggaccagc cggcggcctt tcgggagaac atcctcacac
      961 gctggctatc cgggaccatc acgcagcagg agctggcgga acgggccggg ctggccggcc
     1021 tgaaccccta cgcgctggag tacgcggtgc tggcggctgc cgttctggag caccccggcc
     1081 tggagaaacc tgaggccgtg tccgcccttc agcgggcact cttgccgcgg ctggccgagc
     1141 aggggccttc ggattgcatt gtcgacagct cgggccgcct tctgatcctg atccagggca
     1201 gcgagctgag cgcgcggaca gccctggttc gcgccgcggt gcaggagggc ctgaagaggg
     1261 tccatcagga gcgaggcatc catgcgttcg cctctctggg ggccgtggtc cggggggccc
     1321 agcgagtgga ggtcagttgt cgatccgcct tgctgctcca ggggtatcgc atgttctgtc
     1381 cggcagacag cgtggtgagc gcggatgagg tcccctcttc ggccggccca agagcgggat
     1441 gggactttgc cgagatcgag gagctgatcc gggatggccg cgaagaacaa gcccgcgcct
     1501 acctgcgcgg cgtgctttcc tggttccggg ccgatgacgg gccgccgctg gcggaacgca
     1561 aggcagcgct gaccgacctg atcctgcaca tcgtggcgcc ggtccgccag gccgccggtg
     1621 ggccgcaggg catgcccgcg tccctggcga acctgttcgt gtccatggag gaggcgcaga
     1681 ccgaggagag gctggaggcc tggctgtgct cggtcctgga gaccgccatg agcagcgtcc
     1741 gctcccaaag cgacggcacg acacccatcg ctcgtcacct catggactat atccgtgagc
     1801 actacgatca ggagatctcg ctgaaggtct tcgccgcgga gaaccacgtc aacgcctcgt
     1861 atctgggcca gctcttcaag cgggagacgg ggatgatgtt caccgagtac ctgaaccgga
     1921 tccgcatgcg gcggtcggag gagatgattc gaacggaagg tctgaggatc ggcgagatcg
     1981 cccgccgcgt ggggttcgcg cacgtgagct acttcaacca tgcgttccga aaggcgtttg
     2041 gctcctcccc tctggagtac cgacgcatgg ccgccggcgg tcctccccag aggccgagaa
     2101 gcgaccgttg aagcgccccg cgcccgcccg ggcgtagggc gttgcggagg agccaaaagc
     2161 tacttggtcc cccagagggt gatccagtct ttcacgaggg ccgactcggc gtcctgcatg
     2221 gtgccggtga cacgggccag ctccgccagc atcgtgtcat aggtggcatc gaagtccgcg
     2281 ggcttcccca ggatggccat cggaatgtcc ttgacgggcc ggcacagcgg caggacgata
     2341 cgcaggaaaa tgggaaaatc gccggccccg tcgaccttgg cggactcctg cagctcgaag
     2401 ggaagccggt ccatgcacga gcggatcacg agcacgcaga acgcgttcac catccagggg
     2461 aggacgtaca ccaggaagtc gttgaccagc cggaggttgc gcatgagcag gaacaccggg
     2521 atgaggccgc cggacgcgta cagggtgatg gccaggagcc gggaaaggac ccgccgcgca
     2581 cggagtcccg ggactcattc agggaggtcg ccagcacgtt gaggaacggg tagagcgtca
     2641 ccaccagaat caggaccatg agcagggtgt tgacgatggc gaacgacccg atccggctct
     2701 ttggcacccc gcggcccctt tacatcacgc tctgcccggt cttgcggcgg aacagcccgt
     2761 tggccgtgaa gagcaggccc aggctcacca gggagttgac gacgttgatc gatgcgcccc
     2821 gaccgaagcc gttgctctgc agcccgtact tcagggcgta gaggtccagg acctgggccg
     2881 cgtcatggac cacggggttc atgagctgga accgcttctc gaacccaatg gtgatcaggt
     2941 tcccgatgga gaggatgagc agcacgatga cgatggtgtt gatgccgggg agggtgacgt
     3001 ggatgatctg gcgccaccgc ccggccccgt ccaccgtggc cgcctcgtgc agttccggat
     3061 cgatggccgc gatcgcggcc aggtagatga tcgcgttcca acccagctcc ttccacagat
     3121 cggagcccac gacgatgccc cagaagtacc gcgactgccc catgaagagg accggcttgc
     3181 ctccgagccc atagatgatc tcgttcaccg ggacgttgtc cgtcgagagc ggacgggagt
     3241 ctctggggaa tggggcgcgc ggatttcggt cagctcggaa cgggtgcaac ggcggacgtt
     3301 ctgacgccga cgcaaatcct ggctggcggc gtcgtcgcga tcgcgggagg gctcgtccac
     3361 accgcgatca tcaaggcgga tcacagcatc tggaccaccg ggtacagcag caacggtgag
     3421 ctcggcaacg gctcctccag tggatctctg acgttcgtcc agatccgacg ccccagtctt
     3481 gctccgggcg acccgtagcc taaatcacaa acgagccatc ccgcagtatg gcggtgcgcc
     3541 gacctcccgc cgtaacgccg aagacgtcca cctccggaga accaatcatg aaatcggtgt
     3601 gcaccaggga gacgttgcac cccagcgcgc gcaactcgtc ttccgagagc ttctctccgc
     3661 cctcgatggc atcgggatat ccgttcccca aagcgagatg acaggcggcg ttctcgtcga
     3721 agaggatcga gtggtagact cggccggagc gatggaccgg tgaggagatg tccaccagtg
     3781 agatctcacc cgcgcgtccc gcgttcggat cgatcgtcag gtagcgctcc agcgcgtccc
     3841 tccccctcac cgcgtcacag cgccggacca ggccttctct gaactccatc gtgatgcctt
     3901 ccacgggcac cccgagcacc tcgaccggcc gcgtgcagcg aagcgtgcct tcggtccgac
     3961 gcgcatcggg ggtcgtgaag acctcttccg tcgggaggtt cgggatgaag gtgatccctt
     4021 tctgggaaac ggctcctccg cctatgaacc gtgacctcgg catcaggccg accgtgagat
     4081 ccgtacccgg cccttcaaat cgaagagcct ccaggcgcat cgcgttcagg gcgcgaccgc
     4141 gccgctgcag atgctcagaa tgctccttcc aggcggccac cgggtccgag tgatccagcc
     4201 tcaagatggg cacgaaaagc ttccacagcc cttcgacggg gtccacgcct gccggatccg
     4261 atccgccggc gtcgcccaag accttccgtg cccatcgcgg cgtgggcgcc ggtgccacgc
     4321 accaggtgag ccggttggtc atcacttgtt gctggaagaa gtggcccgcc cgggaggacg
     4381 cctgccgcat cgtggcgagc cgcgccttgt ccacgtccgc caggatatca ggatcctcct
     4441 gtccctcgag gcggagcagc gaccactctt catccaggta ggtctgaagg tccttggcgt
     4501 agaatccggg gacgtagtcc agatggcgtg actccagcgt ctgcgcctgg atgcgcgtca
     4561 ggcgcgcatc ggcgtattcg aggcgcacga accgacctcc aaggcgatag gcttcctcgg
     4621 caacgatcag catgaactcg cgctgtaccg gctccgccag gatgcgcagg ttctgaccag
     4681 gctgcaggtt cacgccgacc tggaggagga cgcgggcgta agagtgcaga aaggcgctgt
     4741 ccatggttgg agtgtcgcga agagcgacgg caaagtccag tgcggaggac ttccgcggaa
     4801 gctgcgatcg tcggaagtgc gcacgatcgc taatgggata aaagcgggtt ggtgacggcg
     4861 atctccgcaa accgcgagaa gtcacgatcc gagctccaca acgccgttac gccgcgatgc
     4921 agacagagcg cggcgacccg ggtgtcatgg accatgccac cgctgattcg agcggcccga
     4981 gcgaggcgcc gaagatgacc gaggtatccg gggccttccc cgattgctac gcagcgcggc
     5041 gattccagcc agcaggagat actctccagc gccgtcccaa tcggggtcgg gggtgcgtag
     5101 atccgcggag cccatcgtgc catcaacgtg cccgacggtc ccggtatgga gacccgtatt
     5161 gactctccca ggcaccggtg gccgaagggc tccgtcagtc cagcctgagc acttcggaca
     5221 gagtcttgat ggcggattca atcgttgccg catccgccat ggtatagttg aggcgcatgg
     5281 tggccagccc ctggccctcc ccggggtaga agaacgtgcc cgggacatag gccacgccgc
     5341 gacggatgca ggcaggggcc atggcgaccg tgtcgagccc ggccggacct tccacccaga
     5401 tgaacattcc gccttctggt ctggaccagt ggaagccatg ttccagatgc ctggccaggg
     5461 cggagagcat ggcctcctga cgaggtctat acaggtcaag gatccgcgga aggtgacgat
     5521 ccaggtgccc gccggagagg tactccgcgg ccatcgcctg accgaaggtg ctcgtgtgga
     5581 ggtccacgcc ctgcttcacg atgaccagcc atcgatccag cggcgccgga gcgatggcga
     5641 atccgatccg cagcccaggc gccaacgtct tggacagggt actggtgtac acgacgttgt
     5701 ccggcgccag ggattggatt ggcggcagag gggtgccgcg atagcgcagg tcgctgtacg
     5761 gatcgtcttc caccagcagc gcctcatggc gctgaaggat ctgggcgatg ctccggcgtc
     5821 gatcgagcgg cagagtccgg cccgtgggat tctggaacgt cggcacgaga tacacgaact
     5881 tcacccgctc ccgggagaga acccgatcca gggactccgg cacggcgcca tcatcatctg
     5941 tctccaccgt aaggtagcgc ggctcgtagg ggttccatgc ctgtagcgct cccagatagg
     6001 tgggagcctc cacggccacc gcatctcctg gagcgatgag cagtttcccc acggcgtcca
     6061 gtgtgccctg ggatccgctg gtcaccagga cggaacgagc agacgccgcg atcccccgta
     6121 gcgcaaggcg ggcggcaagc gcctcctgaa gcggctcgaa accctcggtc cggtcgtact
     6181 gcagggatcg agagccgtag cgggagagca cgcgctcggc gagcgttccc atgagctcca
     6241 tcgggaagct ctcgggcgcc ggtatgccgc cggccaggga aacgatgccg ggttgggaca
     6301 cgaccttgag gatctctcgg atggcgctcg cacccatgag ccgcgtccgg ggcgccagca
     6361 gacgctcgat attcacaaag agcctcctgg atggcattgg ctggcgctac ggtgccaccg
     6421 cgcggccgtg ttgtctacgc cgtctgccag agggacggca gcgcactcag gatgtcttgc
     6481 aggcatgccc ggcgttgcgc agcg
//
LOCUS       DASRZS010000166         5201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_864589, whole genome shotgun sequence.
ACCESSION   DASRZS010000166 DASRZS010000000
VERSION     DASRZS010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864589"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VFH83_04085"
     CDS             complement(<1..638)
                     /locus_tag="VFH83_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6485573.1"
                     /translation="MKRSLVVLLALALLVALIPMAFATGTKESATKTYTIAFMPGVAD
                     PFYTTMERGIQAKAKELGVKVVVGDYPTAWGPEYQLPNLDALMAKGGIDLLLIAPTSK
                     DALVAPLKKINDKGIPVITVDTYLGDGDYSKPSDYSFPLAFIGTDNKLGGKQVAEQLA
                     KLVGEKGKVYVCNTNPDTSSVVDRGAGFKEGIAEFPNIQLVGMDWCMDNQQKA"
     gene            873..2189
                     /locus_tag="VFH83_04090"
     CDS             873..2189
                     /locus_tag="VFH83_04090"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6485574.1"
                     /translation="MVPLPVLRELRSRLPSRPLSSFLERTSGTSRPRRLMGLKWFWID
                     GMFSQLADSFSMDYVPLFALAAGATATSIGLLSSAANLASAITFIPGALLAARLRSRK
                     SIVLSSGYGPARLMLLLLAISPFLFQSGPGATLVALIIALNAVRIGAGSLANPAWTSI
                     VADLVPVDVRGRYLSTRNFAAGLCALGASPAAGWIVSQVNARTGRPLAGYQVTLVVAF
                     ALSLGSTYSYWRIPEPPTLTGTRLKRRVSDLVGLLRRNRVFAWLAATSFLWGVACNLC
                     GPFFNIYLVTRLGGTAASVGISAGTFAISGLVGQAYCGRLVDRRGNRWMLVLTGLMLP
                     VFPVSWLLYRAPWHLYLTNLFSGFIWAGYNLASFNLLLELSPAADRESAIGIYQTAVA
                     ASAVVGPILGGLMVQRLGYASVFVTGSVGRLLAMAAFILLVRPRRT"
     gene            complement(2186..3301)
                     /locus_tag="VFH83_04095"
     CDS             complement(2186..3301)
                     /locus_tag="VFH83_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6485575.1"
                     /translation="MKLPDFQIERFFARYEFNTELLLCASDCQSMSVSELCALEPGAR
                     ERLSDLWLGYTESRGAPSLRGEISRLYRSITAGEVLVHAGAEEGIHLCMHAVLEPGDH
                     VIVQSPCYQSLREIALGTGCEVSPWSMREEDGWAPDPDELDRLVRPSTKLIVVNTPHN
                     PTGYHLPEGVLRRLCRIADERRITLFCDEVYRGLEYRDEDRIPSACDLSERAVSLGVL
                     SKAYGLGGLRIGWMATRDQVLYARMESLKDYTTICSSAPSELLAEIALRHGESIIGRN
                     LGLIRANLSLVGDLLRRHPDLMSWQPPKAGPIAFPRLLRGTIGELSARIAKECGVLLL
                     PGTVYGDRENHFRIGFGRANLPQALARLEEWLSRNRD"
     gene            3359..4357
                     /locus_tag="VFH83_04100"
     CDS             3359..4357
                     /locus_tag="VFH83_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485576.1"
                     /translation="MLEADQRPLRATFVFAILASAALTALIAALGPRLAGVPHLPDQG
                     AAWYFWKLPAPTVWGRLTSWGFYALHQLSVWLLVFLAMRKRRNPHALSGITIGFLAVN
                     LFFILLHILQTHLWYDGLAQDVPIWSSQYSVIVMLVIMLFMLNPRRGLLLGRKFPYSR
                     AGLGFVNRWHGVYISWALVYTFWFHPTEGSIGLLVGFFYMFLLLTQLSMAGTRYHVSI
                     AWITALEFTVGLHGPIIAIQNGYASWPMFAAGFLFMTAFTQQYGFKLPPWGRVAVFAT
                     YGAGVAVMYWFRGYARLYEVLFIPAALYGGAIALALLTRLVRGRGTEPALMEPVRP"
     gene            complement(4347..4709)
                     /locus_tag="VFH83_04105"
     CDS             complement(4347..4709)
                     /locus_tag="VFH83_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485577.1"
                     /translation="MQQISRPPETTPTAVESARQESDAVRTAPAQSGASPAYLAGPAE
                     QGVQGGVGTRTPVIPVENDVEGSLQRASSELSRTPPAPTSEEMRRASDAYQAQAAAQN
                     EQAQRREGDGTRTLDVMA"
     gene            complement(4833..>5201)
                     /locus_tag="VFH83_04110"
     CDS             complement(4833..>5201)
                     /locus_tag="VFH83_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485578.1"
                     /translation="RLSRGSLAVYVACPVCELPVSYLVGLCTWLLEQVKGKHAVLETP
                     LEVEASLFRNGGLRYLTLRNHSSETRDVRYRLSLSRRPARIAELMGGTEIHHFRFLEG
                     QVRFSDTVPAGSIRIYEITM"
BASE COUNT          767 a         1753 c         1685 g          996 t
ORIGIN      
        1 gccttctgct ggttgtccat gcaccagtcc atgccgacga gctggatgtt ggggaactcg
       61 gcaatgcctt ccttgaatcc cgcgccgcgg tccaccaccg atgaggtatc gggattggtg
      121 ttgcagacgt agacctttcc cttctccccc acgagcttgg ccagttgctc ggcgacctgc
      181 ttgccgccca gtttgttgtc ggtgccgatg aacgccagcg ggaagctgta atcgctgggc
      241 ttcgagtagt ccccatctcc caggtaagta tccacggtga tgacggggat gcccttgtcg
      301 ttgatcttct tcagcggggc taccagggcg tccttggaag tgggggcgat caggagcagg
      361 tcgatcccgc ccttggccat gagggcgtcg aggttgggga gctggtactc gggtccccag
      421 gcggtgggat agtcacccac gacgactttc acgccaagct ccttggcctt ggcctggatg
      481 ccgcgctcca tggtggtgta gaacggatcg gcgacaccgg gcatgaaggc gatggtgtag
      541 gtcttcgtgg cggattcctt ggtccccgtg gcgaatgcca ttgggatcag cgccaccaga
      601 agcgccaggg cgagcagcac gaccagcgat ctcttcatgg aaacctcctg tcctttcggg
      661 aaactgtttt tgcgaccagg cgcaataagg caagggccat gccagccgga ccgaccaatc
      721 ttcatcgcct ccaattcttt cccagatata cagatggcgc tcatcgatga gatccgacag
      781 cccgtcgact ggttctccag gtatccaggt ccggatactc cggtagccac ctgctgccca
      841 cggtactgcc cgatctgcta tggtaagccg ccgtggttcc ccttcccgtc cttcgcgagc
      901 tgcgcagcag gcttccttcc cgacccctct cgtccttcct ggagcggacc agcgggacct
      961 cgcgcccgcg tcggctcatg gggctgaagt ggttctggat cgacgggatg ttctcccagc
     1021 ttgcggacag cttctccatg gactacgtgc cgctctttgc cctggcggca ggcgccaccg
     1081 ccaccagcat cggactgctc tcttcggccg caaacctggc cagcgccatc acgttcatcc
     1141 ccggcgcact tctggcggcg agactgcgct cccgcaagtc catcgtgctc tcctccggct
     1201 atggccccgc ccgactcatg ctcctcctcc tggcgatctc cccgttcctg ttccagagcg
     1261 ggccgggcgc caccctggtg gcgttgatca tcgcgctgaa cgcggtgcgc atcggagccg
     1321 ggagcctcgc caatcccgcg tggacctcga tcgtggccga cctggtgccc gtcgacgttc
     1381 ggggccgcta tctgtccacc cgcaacttcg cggcgggcct ctgcgccctg ggcgcctccc
     1441 ccgcggccgg gtggatcgtg tcccaggtga acgccaggac cggccgacct ctggcggggt
     1501 accaggtgac gctcgtcgtg gcgttcgccc tgagcctggg ctcgacgtac tcctactggc
     1561 gcatccccga gcccccgacg ctcacgggca cgcgcctcaa gcgcagggtc tccgacctgg
     1621 tcggcctcct gcggcgaaac cgcgtcttcg cgtggcttgc ggccaccagc ttcctctggg
     1681 gcgtggcgtg caacctgtgc ggtccgttct tcaacatcta cctggtcacg cgcctcggcg
     1741 ggaccgcggc ctccgtgggg atcagcgccg gaaccttcgc catctccggg ctcgtcggcc
     1801 aggcctactg cggccggctg gtggaccgcc ggggcaaccg gtggatgctg gtcctcacgg
     1861 ggctgatgtt gcccgtgttc cccgtgagct ggcttctcta ccgggccccc tggcacctgt
     1921 acctgaccaa cctcttctcg ggattcatct gggccggcta caacctggcc agcttcaacc
     1981 ttctcctgga gctcagcccc gcggcggacc gggagtcggc catagggatc taccagacgg
     2041 cggtcgcggc atccgcggtg gtcgggccca tcctcggtgg tctcatggtg cagaggctgg
     2101 gatacgcgtc ggtgttcgtg acgggaagtg tgggccggct tctcgccatg gcggccttca
     2161 tcctcctcgt acgaccgcgg agaacctagt ccctgttgcg gctcagccac tcctccagcc
     2221 gagccagcgc ctgaggcagg ttggccctgc cgaacccgat gcggaagtgg ttctctcgat
     2281 cgccgtagac ggtccccgga aggagaagca cgccgcattc cttggcgatg cgggcggaga
     2341 gctccccgat ggttccgcgc aggagcctcg gaaacgcgat gggtccggcc ttcggcggct
     2401 gccagctcat gaggtccggg tgccgccgca agagatcacc gaccagcgag aggttggcgc
     2461 gaatgaggcc gaggttcctg ccgatgatgg actctccgtg gcgaagggcg atctccgcca
     2521 ggagctcgct gggggccgaa gagcagatgg tcgtatagtc tttgagggat tccatccggg
     2581 cgtacaggac ctggtcccgc gtggccatcc agccgatgcg cagcccgccg agcccatacg
     2641 cctttgacag cacgcccagg gagacggcgc gctcgctcaa gtcacaggcg gacgggatcc
     2701 gatcctcgtc gcggtactcc agccctcggt agacctcgtc gcagaacagg gtgatgcgtc
     2761 gctcatcggc gatgcggcag agtcgccgaa gcacgccctc cggcagatgg tacccggtcg
     2821 ggttgtgcgg agtgttcacc acgatgagct tcgtggacgg ccgcacgagg cggtccagct
     2881 catcgggatc cggggcccag ccgtcctctt cccgcatcga ccacgggctc acctcgcagc
     2941 cggtgccgag ggcgatctcc cgcagcgact ggtagcacgg agactggacg atgacgtggt
     3001 ccccgggctc gaggaccgcg tgcatgcaga ggtggatccc ctcctcggcg ccggcgtgca
     3061 ccagcacctc ccccgcggtg atcgagcgat acaggcggct gatctcgccc ctgagggacg
     3121 gcgcgccgcg gctctcggtg taccccaacc agaggtccga aaggcgctcc cgcgcgcctg
     3181 gttccagcgc gcacagctcg gaaacgctca tcgactggca atccgaggcg cacagaagga
     3241 gctcggtgtt gaactcgtag cgcgcgaaga agcgctcgat ctggaagtcc ggcagcttca
     3301 tagtttctcc cggggggatg ctatataatc cttggcaccc atgctacgga gggtttcgat
     3361 gcttgaggcg gaccagcgcc ccttgcgggc gacctttgtc ttcgcgatcc tggccagcgc
     3421 cgccctgacg gcgctgatcg cggccctggg ccctcggctg gctggtgtcc cacacctgcc
     3481 ggaccagggg gccgcgtggt acttctggaa gctcccggcc ccgacggtct ggggccggct
     3541 gacctcctgg ggcttctacg cgcttcacca gctctccgta tggctcctgg tgttcctggc
     3601 catgcgcaag cggcgcaatc cgcacgcgct gtccggcatc acgatcggct ttctggccgt
     3661 gaacctgttc ttcatcctcc tgcacatcct gcagacccac ctctggtacg acggcctcgc
     3721 gcaggacgtg ccgatctgga gcagccagta ctccgtgatc gtgatgctgg tgatcatgct
     3781 cttcatgctc aacccgcgtc gaggtctgct cctggggcgg aagttcccgt acagccgcgc
     3841 gggtcttggc ttcgtgaacc gctggcacgg ggtgtacatc tcctgggcgc tggtctacac
     3901 gttctggttc caccccacgg agggctcgat cgggctcctg gttggattct tctatatgtt
     3961 cctgctgttg acccagctct ccatggccgg cacgagatac cacgtctcca tcgcgtggat
     4021 cacggcgttg gagttcaccg tggggctcca tgggccgatc atcgccatcc agaatggata
     4081 cgcatcctgg cccatgttcg ccgccggctt cctcttcatg acggcgttca cgcagcagta
     4141 tggtttcaag ctccccccgt ggggacgcgt ggcggtcttt gcgacgtatg gagccggcgt
     4201 ggcggtgatg tactggtttc ggggatacgc gaggctctac gaggtgctgt tcatcccggc
     4261 ggccctctat ggcggcgcca tcgccctggc tctgctcacc cggctggtgc ggggccgcgg
     4321 gaccgagccc gcattgatgg agcccgtcag gccatgacgt ccagggtgcg ggtaccgtcg
     4381 ccctcccgcc gctgagcctg ctcgttctgg gcagccgcct gcgcctggta ggcgtcagag
     4441 gcccggcgca tctcttccga ggtgggagca gggggggttc gagacagctc cgagctggcg
     4501 cgctggaggc tgccctccac gtcgttctcc accggaatca ccggggtccg agtccccacg
     4561 ccgccctgaa cgccctgctc cgccggaccg gccaggtagg cgggagaggc tccgctctgg
     4621 gccggtgcgg tccggaccgc gtcgctctcc tgacgcgcgc tttccaccgc ggtcggagtg
     4681 gtctccggcg gacggctgat ctgctgcatc gggctgcgat tggaaatcgg gctgatcgag
     4741 gaaaccggtt ctatggccat gggactgacc ctcggtagtc aatgtacacc gcgttcgggc
     4801 gctcgtaaac cgtccctccg gggcggcgcc ggtcacatgg ttatctcgta gatcctgatg
     4861 gaccctgccg gaaccgtgtc gctgaatcga acctgtcctt ccaggaatcg gaaatggtgg
     4921 atctcggtgc cgcccatgag ctcagcgatc cgggccggcc gacgggagag ggagagacga
     4981 tagcggacgt cccgcgtctc gctggagtgg tttcggagcg tcaggtagcg caaccctccg
     5041 tttcgaaaga gggatgcctc cacctccagc ggggtctcga ggacggcgtg cttgcccttc
     5101 acctgctcca ggagccaggt gcagagcccg accaggtagg aaacgggaag ctcacacacc
     5161 gggcaggcta cgtagacggc cagggatccc cgtgacagcc t
//
LOCUS       DASRZS010000167         3532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5481323, whole genome shotgun sequence.
ACCESSION   DASRZS010000167 DASRZS010000000
VERSION     DASRZS010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5481323"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFH83_04115"
     CDS             complement(<1..375)
                     /locus_tag="VFH83_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6485579.1"
                     /translation="MSVLALEGGPKVRKGAWPKTGKRFGTEELRQVKQALEQNTLFYL
                     FGTKTRTLCARMTRLCGRKYALACSSGSAAVHGALKACGVGPGDEVITSPVTDAGTIL
                     GVIYEGALPVFADIDPDSYNITA"
     gene            complement(564..1712)
                     /locus_tag="VFH83_04120"
     CDS             complement(564..1712)
                     /locus_tag="VFH83_04120"
                     /inference="COORDINATES: protein motif:HMM:NF025665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HET6485580.1"
                     /translation="MSGECIVAAYYFPNYHPDPRNAQEHGAGWTEWDVLQAARPRYPG
                     HRQPRVPAWGYEDESQPSVMERKIDAAVQHGVDVFLYDWYWYEDGPFLERGLEQGFLG
                     AHNADRMRFAIHWANHDWLDLHPASRRDCAHRSQRILYPGTLTSQGFERMISHVIQRY
                     FRHPSYWRIDDAPYFSFYDLGSLLKSCGGVEGTAQALARFREAARQAGIPRLHLNQVL
                     WNQGVLPRETGIENADALLKRLGFDSFTSYVWVHHVALDRFPEVDYLHALRAYQQYWK
                     VAVSTASLPYFPNLTMGWDSSPRTVQSDRFENVGYPFTPILSGNTPQRFGEALRALLD
                     QMRANGSPRILTVNAWNEWTEGSYLEPDTQHGMGYLEAIRDALRPGAR"
     gene            2223..3430
                     /locus_tag="VFH83_04125"
                     /pseudo
     CDS             2223..3430
                     /locus_tag="VFH83_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
BASE COUNT          570 a         1127 c         1230 g          605 t
ORIGIN      
        1 cgccgtgatg ttgtacgagt ccgggtcgat gtccgcgaag accggcaagg ctccctcata
       61 gatcacgccc aggatggttc cggcgtccgt cacggggctc gtgatcacct cgtcccccgg
      121 tccgaccccg catgccttga gcgcgccatg aacggcggcg gagccggagc tgcaggccag
      181 cgcgtacttc cttccgcaga gccgggtcat gcgcgcgcag agcgtcctgg tcttggttcc
      241 gaacaggtag aagagagtgt tctgttccag ggcttgcttg acttgccgga gctcctccgt
      301 gccgaaccgt ttgcccgtct tgggccaggc ccctttgcgg accttgggcc caccttccaa
      361 cgcaagaacc gacacgatcg tcctcctcgg gatagggatg cgaagtctcc gggtatagct
      421 cgcggtcagg caggtgggcg tgttcgcgcg tagtcgcgca ggctgctcga tgctggtgaa
      481 cggagcttga cggcagtcta tggagaaggt gctggccgcg tcaagggcgg gcgctccggg
      541 ctatcgcgct ccggcgcccc gggctatcgc gccccgggcc gaagcgcgtc tcggatggcc
      601 tccaggtagc ccattccgtg ctgtgtgtcc ggttcgagat aactcccctc ggtccactcg
      661 ttccacgcgt tgaccgtcag gatccgcggg ctaccgttgg cgcgcatctg atcgagcagc
      721 gcgcgaagcg cctcgccgaa gcgctgcggt gtgttgccgg agaggatcgg tgtgaacggg
      781 tagcctacgt tctcgaaccg atcggactgg acggtccgcg ggctggagtc ccaccccatg
      841 gtcaggttcg ggaaataggg caggctcgcg gtgctcacgg caaccttcca gtactgctgg
      901 taggcccgca gcgcgtggag atagtcaacc tcggggaacc ggtccagcgc cacgtggtgg
      961 acccacacgt acgaggtgaa gctgtcgaag cccaaccgct tcagaagagc gtcggcgttc
     1021 tcgatgccgg tctccctcgg cagcacgccc tggttccaga ggacctggtt gaggtgaagc
     1081 cgtgggatgc ctgcctggcg cgcggcctcc cgaaaccgag ccagggcctg cgccgtgccc
     1141 tcgacgcctc cgcaactctt gagcagggag cccagatcgt agaacgagaa atagggcgcg
     1201 tcgtcgatcc tccagtagga tggatggcgg aagtagcgtt ggatcacgtg ggagatcatc
     1261 cgctcgaagc cttgggaggt gagggtgccc gggtagagga tgcgctggga gcggtgagcg
     1321 cagtctcgcc gggaagccgg atggaggtcc agccagtcat ggttggccca gtggatggcg
     1381 aaccgcatgc ggtcggcgtt gtgggccccc aggaatccct gctccaggcc acgctccagg
     1441 aaaggaccgt cctcgtacca gtaccagtcg tagaggaaga cgtccactcc gtgttgcacc
     1501 gcggcatcga tcttgcgctc catgacggac ggttgcgact cgtcctcgta tccccaggcg
     1561 gggacccggg gttggcggtg cccggggtac cggggtctgg ccgcctgcag cacgtcccat
     1621 tcggtccacc ccgccccgtg ctcctgggcg tttctcgggt ccggatggta attcgggaag
     1681 tagtacgcgg ccacgatgca ttcaccactc acggggaagt cctcctgacg gccaggctat
     1741 ggatggccga aggagccacg aggacgggga cgtccgacag gctcctgggc tttcgcccac
     1801 gctacccgaa cccaccgttc catgggaagt acgagaccgg gaacgttgag atgctaccgg
     1861 tggcgcacta tattccgtct aaaggcgaga cggggccgac gcgccccagc cccgcatcta
     1921 ccatacccga ttcgaaagaa ggtggccgca tgcaggaagt ggatccccgc cgcggtgtgc
     1981 cgcgcgcgga gagctacctc gatcctgcct cagagaccta cgcatcgcgc ttcttcgacg
     2041 cgtgcttcga gcgtgagctg acccggtgcg tccgctacgg ggactccgtg accctgggcc
     2101 tgctgtcggt gcgcggcgcg gacgcgtcgg gcggaccctc gccggacctg ggtcgtctga
     2161 tcctgtccag catacgggag gtggacctgg ccgctttccg cggcggggat tgctacacgc
     2221 tgatgctggt caaggcctcc gcggacgtgg ccgccatcgt cggagagcgg gtcctgaacg
     2281 cgctggccga ccgctgcggc accgacgggg ccgcctggct ggggctggca tcctaccctc
     2341 gcgatggctc cagctccgcc gcgctccagg agtgcgcggc acgggcgctc gggaaagccc
     2401 gggaaaccgg ccccaaccgc atctaccatt tccctcggga gggtggcgcc gctgcctcgg
     2461 accagccgag gatcctcgtg gtggacgacg acccccgcaa cgtcaagctc ctggaggccc
     2521 tgctcctgcc gatgggatac gaggtcgtaa aggcgtcgga cggaccggcg gcgctggagg
     2581 cggtccaaca ggccgggatc gatgtggtcc tcctggacgt catgatgccg ggaatgagcg
     2641 ggtacgatgt gtgccgccgg cttaagaaca tggagggcac ccggaccctg ccggtcatcc
     2701 tggtgaccgc gctggaggac gtggactcca aggtgaaggg gatccaggcc ggcgcggatg
     2761 agttcctcac caagccggtg gtgcgggagg agctgctggc gcggacgcgg tccctcgtca
     2821 acctgcaccg gctgagccgc aacctggtga acatcgagag cgcgctgtac tccctggcca
     2881 acgccgtgga ggccaaggac agttacacgc tcggtcatac gcagagagta gcggccctcg
     2941 ccgtggccgt gggcggagcc ctggggctcc gggagaagga cctctccgcg ctccgcctgg
     3001 gcgggatcct ccatgacgtg ggcaagatcg gagtccctga ggcggtgctg aacaagggcg
     3061 gcccctggac gagaaggagt ggaagctcat gcgcgcccac accgagctcg gctataccat
     3121 ctgtcttccg ctcgtggatt ccatcggacc ggccctggac gtgatccgcc accatcacga
     3181 gaagctggac ggctcttcgt atcccgacgg tcttcggggg aacgaactgt cgatgatcgc
     3241 ccgcgtgatg ggcgtcgtgg acgtctacga cgcgctggtt tccgatcggc cgtatcggaa
     3301 ggccatgtcc cgcgagaagg ccctggagat ccttcgcgcc gaggtgcagt ccggcaagat
     3361 cgatggcagc gtcgtgacgg ccctggagcg gattgtcgcc cgggacgagc gcacggcgtc
     3421 cggaggatag ggatgtcgat tcgcgcaaaa ctgctcctgc tgaccggcgt cgcgctggcc
     3481 gcggtggtcg cgggattcgc gcttctggac cgtctggtgc tcttgcgaag ct
//
LOCUS       DASRZS010000168         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2019340, whole genome shotgun sequence.
ACCESSION   DASRZS010000168 DASRZS010000000
VERSION     DASRZS010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2019340"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFH83_04130"
     CDS             <1..354
                     /locus_tag="VFH83_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HET6485581.1"
                     /translation="LRRIFEIFEKHETPVDLIATSEVSVSVTIDTPTAVDAIARDLAE
                     IGTVGVETQKSIVRLVGQDLWKDTVFLARVFSAMGDIPVRMISLGASDINLSLVVPVE
                     STEESVRRLHKEFFG"
     gene            446..1288
                     /gene="dapF"
                     /locus_tag="VFH83_04135"
     CDS             446..1288
                     /gene="dapF"
                     /locus_tag="VFH83_04135"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET6485582.1"
                     /translation="MEIDFLKMQGCGDDVIVVDGARLPPEAVPHLPRLAGRMLDRGTG
                     VGGMFLILLGRGQESTVRCFGPQGNETDLPCNGARCVARYATDSGAVTAERFSLRTAS
                     GGIRIQIIDSANVRVDMGRPFGPEQQAQIEESPTGSFTRSILVDGKGLTYTPVSLGRS
                     YAMVFVPSFSFPLRKKARSIAAEKEFPAATGIGFVQVCSRERLRLRFWEPDPGDPGDA
                     CVCAAAALVASTVNGFTEREVFVRLPGGEVFLQWEEPDNHIWLTGPASYVFTGSYDFE
                     PDQE"
     gene            1294..1734
                     /locus_tag="VFH83_04140"
     CDS             1294..1734
                     /locus_tag="VFH83_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET6485583.1"
                     /translation="MENFIDRFADFLRSVLGGDKEEDTPSSGRQPRSRFVDPDVEEAW
                     QELDDYMRGGDSSARSADARSRDGSRTRSAPRPPNESLRQDYANLEVPFGASIEEVRR
                     SYKNLILKYHPDKHAGNAEEQRVALEITKKINASFERIRASHEG"
     gene            complement(1774..>2602)
                     /locus_tag="VFH83_04145"
     CDS             complement(1774..>2602)
                     /locus_tag="VFH83_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6485584.1"
                     /translation="LSLNAAIEAARAGESGRGFTVVAQQVRKLADRSSGAASEIADLT
                     GLVLDGVRRVSSEAQGALEGIAQLQKELAGLAESVQSVTELSGGTVGALAKADASVGT
                     TLGLVTEASRRIEEASSAAGTLQEIVTQLTRSVARLRAGRAAPATPPDTTAAVSGAPA
                     QESSRPVGIAAAELRDRRASPLDEVNPAPSEEVPAAEALSVEPPPEGAFPAAAPAEEA
                     LPAEEALPAEAAFPAEEAPAAEVAIEELVSLEVSANEAPASASQEPSELVDLEVPKP"
BASE COUNT          436 a          880 c          848 g          438 t
ORIGIN      
        1 ctccgccgga tcttcgagat cttcgagaag cacgagactc cggtcgacct gattgccacg
       61 tccgaagtct ccgtgtccgt cacgatagac acgccgaccg ccgtggacgc catagcccgt
      121 gacctggcgg agatcgggac cgtcggggtg gagacgcaga agagcatcgt ccgcctggtg
      181 ggtcaggatc tgtggaagga caccgtcttc ctggcgcgcg tgttctcggc catgggtgac
      241 atcccggttc ggatgatctc cctgggcgcc tcggacatca acctctctct cgtggtcccg
      301 gtagaatcga cggaagaatc ggttcgacgg ctgcacaaag agttcttcgg ctgatgcctt
      361 cgaacccccc ggtctctgtc gtcccctcgg gccccttgcc gtggggcacg ggccggggta
      421 gcctttcaac caccggcgca gggcaatgga aatcgacttc ctcaagatgc aggggtgtgg
      481 agacgacgtg atcgtcgtgg acggagcccg tctcccgcca gaggcggtgc cccacctccc
      541 tcgcctggcc ggccgcatgc tggatcgcgg gaccggagtt gggggcatgt tcctgatcct
      601 cttgggccgg gggcaggagt cgaccgtgcg ctgtttcggc cctcagggca acgagacgga
      661 tctcccctgc aacggcgccc ggtgcgtggc ccgctacgca acggactcgg gtgccgtcac
      721 cgcggagcgc ttctctctcc gcacggcctc gggcggcatc cgaatccaga tcatcgacag
      781 cgcgaacgtc cgcgtggaca tggggcggcc attcggcccg gaacagcagg cccagatcga
      841 ggagagcccc acgggatcgt ttacccgcag catcctggtg gacggcaagg gactcaccta
      901 tactccggtc tccctcggtc gatcctacgc catggtattc gttccgagct tctcctttcc
      961 gctgcgcaag aaggcgcgtt ccattgccgc cgagaaggag ttccccgcgg cgacgggcat
     1021 cggattcgtg caggtgtgct cccgcgagag gctacggctt cgcttctggg aaccggatcc
     1081 gggagatccg ggggacgcct gcgtgtgcgc ggccgccgct ctcgtggcct ccacggtcaa
     1141 cgggttcacg gagcgggagg tgtttgtccg tctgccgggg ggggaggtgt tccttcagtg
     1201 ggaagagccg gacaaccaca tctggctgac gggacccgcc tcttacgtgt ttacgggtag
     1261 ctacgacttc gaacccgacc aggagtgacc aaggtggaaa acttcatcga ccggtttgcc
     1321 gacttccttc gctccgtgct gggcggagac aaggaggagg acaccccgag cagcgggagg
     1381 caacctcggt ctcggttcgt ggacccggac gtggaagagg cctggcagga gctggatgac
     1441 tacatgcgcg gcggcgattc aagcgcccgc tccgcggatg cgcgctctcg ggatggatcg
     1501 cgaacccggt ccgcgccgcg gcccccgaac gagtccctgc ggcaggacta cgccaacctg
     1561 gaagtgccct tcggggccag tatcgaagag gtccgtcggt cctacaagaa cctcatcctc
     1621 aagtaccacc cggacaagca cgccggtaat gctgaggagc agcgcgtcgc cctggagatc
     1681 accaagaaga tcaacgcgtc cttcgagcgc atccgggcca gtcacgaagg atagaccgca
     1741 gagccgcgcc ggaaaaagca agccaggatg gcgctacggt ttagggactt ccaggtcaac
     1801 gagctcagac ggctcttggg atgccgatgc aggggcctcg ttggctgata cctccagtga
     1861 caccaattct tcaatcgcca cctctgcggc cggcgcttcc tccgccggga acgctgcctc
     1921 cgccggaagc gcttcctccg ccggaagcgc ttcctccgcc ggagccgccg ccgggaacgc
     1981 tccttccggg ggcggttcca ccgacagggc ttctgcagcc ggtacttctt ctgacggggc
     2041 tgggttcact tcatccaggg gcgaggcccg ccggtcccgc aactctgcag cggcaatccc
     2101 cacgggacgc gacgactcct gggcaggagc tcccgacacg gcggcggtag tgtccggagg
     2161 cgtagcgggc gcggcacggc ctgcgcgcaa ccgagccacg gatcgggtga gctgagtcac
     2221 gatctcctgg agcgttcccg cggcactcga tgcctcttcg atgcgccggg aggcctccgt
     2281 taccaggccc agcgtggtcc ccacggaggc gtccgccttg gccagcgcgc ccaccgtgcc
     2341 accggaaagc tcggtgaccg actgcaccga ctccgcaagg ccggccagct ccttctggag
     2401 ctgcgcgatg ccctccaggg cgccctgggc ctccgaggaa acccttctta caccatccag
     2461 gaccagaccc gtgagatccg cgatctccga tgcggccccc gagctgcgat cggcaagctt
     2521 cctcacctgc tgagccacca cggtgaaccc tcggccggac tccccagctc gagctgcttc
     2581 aatggccgcg ttgagggaca gc
//
LOCUS       DASRZS010000169         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_480364, whole genome shotgun sequence.
ACCESSION   DASRZS010000169 DASRZS010000000
VERSION     DASRZS010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480364"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VFH83_04150"
     CDS             <1..900
                     /locus_tag="VFH83_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6485585.1"
                     /translation="TQSDKVVAMFGFSDSDMTMAAAPIAAKAGIVFVTSGATSPKLPD
                     QVPDYLYLACFGDNVQAAAGAEYAINTLKAKTVYLLIDKGMEYTLLLGKYFQERFVEL
                     GGKVVLQDNYQTGDKDFSAQITRLKGQKAKADLLYIASGPDDVGTIVKQFRDAGIKTP
                     IMGGDGYDTTNLVQVGGAGAENVYFTTHSLMDANLGTDAVKKFIAAYQAEYKNPPENA
                     FAGLGYDTVKLLADSIKRAGSADPKAIRTALQATKDLPGVTGSITYQEGSRIPQKGVT
                     VILVKDGKFTLAAEVVPQKVPAP"
     gene            1008..1778
                     /locus_tag="VFH83_04155"
     CDS             1008..1778
                     /locus_tag="VFH83_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET6485586.1"
                     /translation="MKKNPIEVQHLTAADFRQKPFDTVILPLGSLESHGGHLPFGTDA
                     LTAYLLALDIAARLSRVAVLPPVNYGVSEHYKDFSFTVTLGFETQSAVIRDILESVYR
                     EGIRKVFIMNGHDGNISPIEIASRSAKVAHPDFKIVSLDAWWMMIGDLLPKGFFEVWN
                     GLGHGGEGETSICLELFPELCDPSKAAGVVPQLPKNLDVKWLFKELTNTGATGDPTRG
                     SAAKGKKMRQVLVDAMVEALKDLDQRGWDWRSPEVRKG"
     gene            1883..>2475
                     /locus_tag="VFH83_04160"
     CDS             1883..>2475
                     /locus_tag="VFH83_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HET6485587.1"
                     /translation="MADSTVTRSDLQSGLRSVGLRGGDLVFLHASLSAFGRVEGGART
                     VVEALMDTLGSEGTLVAPIFRTFFLDGPDQVWDRERSPSLMGAVTEAVRTWPGALRSK
                     HAIHPIAAVGPLADELTEPDNTTDFSFDSPFSLLLERNARILLLGVTLNRCTMVHLLE
                     ERLLIPYRRWVTRTGTVIQDGNSRRMDYPFLERYPGV"
BASE COUNT          454 a          820 c          815 g          386 t
ORIGIN      
        1 acccagagcg acaaggtggt ggcgatgttc gggttcagcg acagcgacat gaccatggcg
       61 gccgccccca tcgcggcgaa ggccgggatc gtgttcgtga cctcgggggc cacctcgccc
      121 aagctgccgg accaggtccc cgactacctg tacctggcct gtttcgggga caacgtccag
      181 gccgccgcgg gtgcggagta cgccatcaac accctcaagg ccaagaccgt ctacctgctc
      241 atcgacaagg gcatggagta cacgctgctt cttggcaagt acttccagga gcggttcgtg
      301 gagctgggcg gcaaggtggt cctgcaggac aactaccaga cgggcgacaa ggacttctcc
      361 gcccagatca ccaggctcaa gggccagaag gccaaggcgg accttctgta tatcgcttcg
      421 ggacccgatg acgtcggaac catcgtgaag cagttccgcg acgcgggcat caagactccc
      481 atcatgggag gcgacggcta tgacacgacc aacctggtcc aggtcggcgg cgccggcgcg
      541 gagaacgtct acttcaccac gcactcgctg atggacgcca acctcggcac ggacgcggtg
      601 aagaagttca tcgcggccta tcaggcggag tacaagaacc cgccggagaa cgcgttcgcg
      661 gggctggggt atgacacggt gaagcttctt gctgactcga tcaagcgggc gggctccgcg
      721 gatcccaagg ccatccggac cgcgctccag gcgaccaaag acctgcccgg ggtgaccggc
      781 tccatcacct accaggaagg cagccgcatc cctcagaagg gtgtgaccgt gatcctggtc
      841 aaggacggga agttcacgct ggccgcggag gtggtgcccc agaaggtacc ggctccctga
      901 cgccgagtct caaccaaagc acggagcggg ccgcgaaagc ggcccgcgat ctgtcggggc
      961 gcgtcccgca gacgcaacgt ccccaccacc ccaccggagg atagcgcatg aagaagaacc
     1021 ccatcgaggt ccagcacctc acggccgcgg acttccgcca gaagcccttc gacacggtga
     1081 tccttccgct aggctccctg gagagccacg gcggtcacct gcccttcggc accgacgccc
     1141 tcacggcgta tctcctcgcc ctggatatcg ccgcacggct gtcccgggtc gcggtcctgc
     1201 cgccggtgaa ctacggggtc agcgagcact acaaggactt ctccttcacc gtgacgctcg
     1261 gattcgagac gcagagcgcg gtgatccggg acatcctgga atcggtctac cgggaaggca
     1321 tccgcaaggt cttcatcatg aacggccatg acggaaacat ttcgcccatc gagatcgcca
     1381 gccgctccgc caaggtggcg catccggatt tcaagatcgt gtccctggac gcctggtgga
     1441 tgatgatcgg cgacctgctg cccaaggggt tcttcgaggt ttggaacggg ctgggccacg
     1501 gcggagaggg ggagacctcg atctgcctgg agctattccc cgagctctgc gatccctcca
     1561 aggcggcggg cgtggtccct cagctcccca agaacctgga cgtgaagtgg cttttcaagg
     1621 agctcacgaa cacgggcgcg acgggagacc ccacccgggg atccgcggcc aagggcaaga
     1681 agatgcgcca ggtcctggtg gacgcgatgg tggaggccct caaggacctg gatcagcgcg
     1741 gctgggactg gcgatcaccg gaagtgcgta aggggtagag gcgccgaggg ccaaaggccc
     1801 ctagaccgct cgtcccgggc caggaagacc ggcggccgat gccccccttg cctcccggca
     1861 gcggagtcgg caggatcgcg gcgtggcaga ttccaccgtc actcgatcag acctgcagtc
     1921 cggtctccgg tcggtcggcc tccgcggcgg cgacctggtc ttccttcacg cgtccctgag
     1981 cgcctttggc cgcgtggagg gcggggcgcg gacggtggtg gaggcgctga tggacaccct
     2041 ggggtccgaa ggcaccctgg tggcgccgat cttccggacc ttcttcctgg atggccccga
     2101 ccaggtatgg gaccgcgagc gctcgccatc cctgatgggc gcggtgacgg aggcggtccg
     2161 gacgtggccg ggtgcgctcc gcagcaagca cgccatccac ccgatcgccg cggtgggacc
     2221 cctggcggac gagctcacgg agccggacaa caccacggac ttctccttcg acagcccctt
     2281 ctcgcttctg ctggagagga acgcccggat ccttctcttg ggggtcacgc tcaaccgatg
     2341 cacgatggtc catctgctgg aagagcgcct tctgatcccg taccgcagat gggtcacgcg
     2401 gaccggcacg gtcatccagg acggcaactc ccggcggatg gactacccgt ttttggagcg
     2461 ctacccgggt gttga
//
LOCUS       DASRZS010000170         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1248068, whole genome shotgun sequence.
ACCESSION   DASRZS010000170 DASRZS010000000
VERSION     DASRZS010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248068"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VFH83_04165"
     CDS             complement(<1..544)
                     /locus_tag="VFH83_04165"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485588.1"
                     /translation="MSFLISNLATRRIARWLGVLLTLTAATAGYAATSTGPKAYIGLF
                     KDNAVAVLDTATNQLLTTIPIPPGPHGLVVSPDGATVYASSDGDSRVSVIDTSQDAVV
                     ASIPVGTSPHGLAITPDGKTVLAAVFGANAVVFIDTATRTVQGQALVANPHNIAISPD
                     GRTAYVAAQGQDALALVILDV"
     gene            complement(628..1674)
                     /locus_tag="VFH83_04170"
     CDS             complement(628..1674)
                     /locus_tag="VFH83_04170"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6485589.1"
                     /translation="MPSVPLRAAFLGFRHPHVWDAFQRLQERPDVEVVACCEEDPATR
                     ESLKRDGKVTITHTDHAALLAEVETDFVVVGDFYGARGRLVADSLRAARHVLTDKPLC
                     TRLSEYQEIAALAGPGPAGNGRPVLGMMLDMRDSGNFRALRDLVRQGAIGDAQAISMG
                     GQHPLLRGTRPAWYHEKDRHGGTINDIAIHGVDAIRWITGREFDRVLAARAWRGNAPE
                     SSAMKSAAQCMLTLDNGCGVLADVSYLSPDSHRYTLPLYWRMTLWGTRGVIETANNAS
                     GVTLYQEGKTSGEVVPPAPAQPGGYLESFLSQVRGSPIPDALTPAEVLRASYIVLKTQ
                     EAADRGLTNVDLRP"
     gene            complement(1689..2753)
                     /locus_tag="VFH83_04175"
     CDS             complement(1689..2753)
                     /locus_tag="VFH83_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010273411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6485590.1"
                     /translation="MSKLRVGIIGQGRSGHDIHVVALTRHAMDLYDVVAVADPVQERL
                     HSDLLGKDCASYTDYRQLFGRKDIDLIVNASPSHLHVPITIEALKAGFNVLCEKPLAR
                     KASEVDKLVATARKAGKVLAIFQQSRFAPHFQQIRSVIQSGVLGRIVMVKIRYNGFSR
                     RWDWQTLQEYNGGSLLNTGPHPMDQALQLFGTDLMPTVACAMDRANTFGDAEDVVKVM
                     LSGKGRPIIDVEICSCSAYNGATYQVNGTNGSISGSQDHLDWKYFKPEEAPPQKLIRE
                     PLPGRAWCTETLRWHTGSWDIPKDGIELFDRMAKAFYTDLHEALTQGRPLTVRPEHVR
                     RQIEVIEECHRQNPLSRLKA"
     gene            complement(2832..>3224)
                     /locus_tag="VFH83_04180"
     CDS             complement(2832..>3224)
                     /locus_tag="VFH83_04180"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET6485591.1"
                     /translation="TLQRLAVQRSESALLMDTASDITVIRVPSVAGPAAQTHGSYRGR
                     PGTPMAILALHYFPPAPAGKVYQAWVRTNGAWTSMGVAYPDANGMGVVVGEGQAYRVL
                     PQVIEVTEEPRGGSPSPTGPPVIYLERP"
BASE COUNT          483 a         1065 c         1116 g          560 t
ORIGIN      
        1 ccacgtccag gatcaccagc gccagcgcgt cctgcccctg cgccgctacg taggccgtgc
       61 ggccgtccgg gctgatggcg atgttgtgcg ggttggcgac cagcgcctgg ccctggaccg
      121 tccgcgtggc ggtgtcgatg aacaccaccg cgttggcgcc gaacacggcg gccagcacgg
      181 tctttccgtc cggcgtgatt gccagcccgt gcggagaggt ccccaccggg atgctggcaa
      241 ccacggcgtc ctggctggtg tcgatcacgc tgacgcggga atcgccatcg ctgctggcgt
      301 agacggtggc gccgtccggg ctcacgacca ggccatgcgg ccccggcggg atcgggatgg
      361 tggtgagaag ctgattggtc gcggtatcga gcacggctac cgcgttgtcc ttgaagagac
      421 cgatataggc cttcggtccg gtagaggtgg cggcgtagcc cgcggtggcc gcggtgagcg
      481 tcagcaggac gccgagccat cgggcgatcc tccgcgtcgc gaggttcgat atcaggaaac
      541 tcatcggttc cactccttgg tgtgtagagg ccggccttgc ggccgacccg agttatccac
      601 acctacaccg gggagaggag cttggattta cggtcggagg tccacgttgg tgaggccacg
      661 gtcggcggcc tcctgggttt taaggacgat gtagctggcg cgcagcacct cggccggagt
      721 cagagcgtcg gggatgggac tcccccgtac ctgggagagg aaggactcca gatacccgcc
      781 gggctgcgcg ggcgccgggg gcaccacctc gccgctcgtc ttcccttcct ggtagagggt
      841 cactccggac gcgttgttgg cggtctcgat cacgcctcgg gttccccaga gggtcatccg
      901 ccagtacagg ggcagcgtgt accggtggct gtccggcgac aggtaggata cgtcggccag
      961 gaccccgcag ccgttgtcca gcgtgagcat gcactgcgcg gcgctcttca tggcggagga
     1021 ctccggcgcg ttcccgcgcc acgcccgcgc ggccaggacg cggtcgaact cgcggcccgt
     1081 gatccaccgg atggcgtcca cgccgtggat ggcgatgtcg ttgatggtgc cgccgtggcg
     1141 atccttctcg tggtaccagg cgggccgcgt gcctcgaagg agcggatgct gaccgcccat
     1201 gctgatggcc tgcgcgtcgc cgatggcgcc ctggcggacc aggtcccgca aggcgcggaa
     1261 gttcccggag tcgcgcatat ccagcatcat gcccaggacg gggcgtccgt tgcccgccgg
     1321 acccggcccg gccagcgcgg cgatctcctg atactcggag agacgcgtgc acagcggctt
     1381 gtccgtgagc acgtgccttg cggctctcag gctgtccgca accaggcgcc cgcgggcgcc
     1441 gtagaagtcg cccaccacga cgaaatcagt ctcgacctcc gccaggagcg cggcgtggtc
     1501 ggtgtgggtg atggtgacct tcccgtctct cttcagggat tcccgtgtgg cggggtcttc
     1561 ctcacagcag gccacgacct ccacgtccgg gcgctcctgg agcctctgga acgcatccca
     1621 gacgtgggga tgacggaacc ccaggaacgc cgcgcgcagg ggcacggacg gcaccgggcc
     1681 caccggactc aggccttcag acgggacaag gggttctggc gatgacactc ctcgatgacc
     1741 tcgatctggc gtcgcacgtg ctcgggcctc accgtcagag gtcgaccctg ggtcagcgcc
     1801 tcatggaggt ccgtgtagaa cgccttcgcc atccgatcga agagctcgat cccgtccttg
     1861 gggatgtccc agctcccggt gtgccacctc agggtctcgg tgcaccacgc cctgcccggc
     1921 agcggctcgc ggatgagctt ctgaggcgga gcttcctcgg gcttgaagta cttccagtcc
     1981 aggtggtcct ggcttcccga gatgctcccg ttggtcccgt tgacctggta ggtggcgccg
     2041 ttgtacgcgg aacagctgca gatctccacg tcgatgatgg gccggccctt tccgctcagc
     2101 atcaccttca ccacgtcctc cgcgtccccg aaggtgttgg cgcggtccat ggcgcaggcc
     2161 acggtgggca tcaggtcggt gccgaagagc tggagcgcct ggtccatcgg atgcggtccg
     2221 gtgttcagga ggctcccgcc gttgtactcc tggagggtct gccagtccca ccgacgggag
     2281 aacccgttgt agcggatctt caccatcacg atcctgccca gcacgccgga ctggatcacg
     2341 gagcggatct gctggaagtg aggggcaaag cgcgactgtt ggaagatcgc cagaaccttc
     2401 cccgccttgc gggccgtggc caccagcttg tccacctccg aagccttccg cgccagcggc
     2461 ttctcgcaga gcacgttgaa gccggccttg agcgcctcga tggtgatggg aacgtggagg
     2521 tggctcgggg aggcgttcac gatcaggtcg atgtccttgc ggccaaagag ctgtctgtag
     2581 tcggtgtaag aggcgcagtc cttgcccagc aggtccgagt gcagccgctc ctggacgggg
     2641 tccgccaccg ccaccacgtc gtagagatcc atcgcgtgcc tggtgagcgc gaccacgtgg
     2701 atgtcgtgcc cacttctccc ctggccgatg atgccgaccc gaagcttgct cactgcaacc
     2761 tcctcgtctg gatgcgtagc gctgtgatat cccggacccg gcgggtccgt caaggaagga
     2821 ggcgagcggg gctacggtcg ctccaggtag atgaccggag gcccggtggg agaggggctg
     2881 cccccgcgcg gctcctcggt gacctcgatg acctggggga gcacacggta ggcctgtcct
     2941 tcgccgacga ctacgcccat gccgttcgca tccgggtatg ccacgcccat cgacgtccag
     3001 gcgccgttgg tccggaccca ggcctggtag accttccccg ccggggccgg tggaaagtag
     3061 tgcagggcca ggatagccat cggagtcccc ggtcggccgc gataggatcc atgggtctgg
     3121 gccgccggac ctgcgacgga tggcacgcgg atcaccgtga tgtcgctcgc ggtatccatg
     3181 agcagcgcgc tctcggatct ctggaccgcg agccgctgca aggt
//
LOCUS       DASRZS010000171        10188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4715717, whole genome shotgun sequence.
ACCESSION   DASRZS010000171 DASRZS010000000
VERSION     DASRZS010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10188
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4715717"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..116
                     /locus_tag="VFH83_04185"
     CDS             <1..116
                     /locus_tag="VFH83_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HET6485592.1"
                     /translation="GAESEALRNQLEKAVELEDYERAAELRDQIRKIESNV"
     gene            109..1209
                     /locus_tag="VFH83_04190"
     CDS             109..1209
                     /locus_tag="VFH83_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HET6485593.1"
                     /translation="MFEFTGLSDMGGWMRERGEPEAAAAGTTDPSASVVISSRLRLAR
                     NLTGFPYPGRCPADVEQQVRERILGAFRRLPNAEDFAILSLDDLSSLERRILFERNLI
                     SQGYSLEKHKALVLSSDESVAATIGEEDHLRLVGLGNGASLEKLLARLDDLDSALEPA
                     LPYAAASEWGYLNTAITNLGTGLKASLMLHLPALSMSGLIKRAAKASAQLGLSIRGFF
                     GEGSESLGDMYLIANEVTIGLTEREIIGNLGRIAVQLAGYESRTREELMEKRRVDVED
                     RVYRAWGLLTWCRSITLREAIEALSALRMGVGLGLITTVEASTITALLFLSQKSHLQR
                     VLQSHADTVDGKLIDYTRAKIIREALTKTRGG"
     gene            1214..3742
                     /locus_tag="VFH83_04195"
     CDS             1214..3742
                     /locus_tag="VFH83_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET6485594.1"
                     /translation="MFKGLTQRAQKVLTILAQEEAKRFHSDQLLPEHIILALLKDGEG
                     VAVKALQRIKVSIAEMQSEIEKSLPKGKGGLILGDVPPSKRGQKILEDSAEEARNMGH
                     EYIGTEHLLLASIKEQGSIVARYLSRFNVTPNGLREAILQISGSGEYKGSPSAAGKKK
                     AQAPTRKPTPTLDEFGRDLTSFARDNKLDPVIGREKEIQRVIQILARRTKNNPVLIGE
                     PGVGKTAIVEGLAQKIVDGTVPEVLIGKRVLTLDLASLVAGTKYRGEFEERLKRVMKE
                     ILGAGNVVLFIDELHTIIGAGGAEGAIDASNMLKPALSRGELQCIGATTLNEYKKHIE
                     KDAALERRFQPIYVSEPSVEETTEILKGIKRNYEEHHHVVYTPAAIEAASTLSRRYIT
                     ERYLPDKAIDLIDESGSRRRIRNSIRPRELADLEKEIERLNLEKTNLVNTQNYEKAAA
                     VRDMVRQLKTKVDDIKKSWESTVSVEENVVDVEDIQQVISDMTGIPLSRIAQSESEKL
                     LHIEEELHKKVIAQEEAISVIASAIRRSRTGLSSARRPLGSFIFLGPTGVGKTLVAKT
                     LAEFLFGDADALVRIDMSDFMEKHNVSRLVGAPPGYVGYEEGGLLTEKIRRRPFSVIL
                     LDEIEKAHPDVFNILLQVLEEGQLSDNLGHSVSFRNAVLIMTSNLGAREINKDSSLGF
                     QSEESGMNFKDIRNYVMSEVKRAFNPEFINRIDEIVVFHNLDRTHLSQIFDLMLKEIS
                     VRLAEQKITISVKKRAREYLIEKGFDEKFGARPLRRLLQKEVEDPLSLEILKGKFKSG
                     DAIEIEVRGERIAFRKVEPKKILAAPAQPAGLLPGEKEPEKVTT"
     gene            3797..6790
                     /locus_tag="VFH83_04200"
     CDS             3797..6790
                     /locus_tag="VFH83_04200"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6485595.1"
                     /translation="MTRRAAWVLGALLLVTPCFADTAADLLATGKKAFTDGLYPVAAR
                     SFQRILNEFPGSDGATEAEYLLGVAFFYSGDWASAQSTLLGFRTHHPGTALADRTSYW
                     LGAASLKQGNYPAALGYLEQSLAPSGSGTPNPYRLQSLLLTGVAQESLGRDAEAAAAY
                     RLLISDPGAGGLAPEAVFRLAGTAYRAGDFAGASDLYGRVVLSYPGSPFVADSLFFLG
                     ESLLSLGNLADAEQRYTTLLSLYPDSPYREAALFRLADIAWRGSRATDALARLDTLNQ
                     QFPAGAYRGSAYRLRGDILLSQQAYDLALNQYQGAVSLLPQGTERQSALYSMGIAQLM
                     LGKREQAAQSFKAAQVGGSSDIARKAARQAALLDASDDAPDQGIPSLQSYVGGEDQGP
                     GAQGPGAPIAQANGDARTLLASLQEQKGDYDGAAVTWSSLIERAPDPASLPEYLYRRA
                     QDLLRAGRPSAALDDLQRVLRDYPSSAWSAQSSYAVGYAYSLSSEYARALPYFRSAAT
                     GAGTTATAAGAADVAARSRLAVGVCLFDMGDFPRALSSLQDLLANLPAGLPQATVVLY
                     VGRTLYRMERLGEAAQTLGQAASLFAASPPRPAGEAGFLDPVGQAADAVYWQAWALFR
                     LGRFAEARDAFLSIPTRFPSDTRAAEALLRAGICDTLSADDPDAVPLFDRVLASQASG
                     IDDLREQALYEKGWALSRQGRSQDSAADFELLARRYPDGAFAPQAFLKLASTALENKR
                     FADALAGFQRVAKDFPESVAAPRARYLIAETRLESGDAQGAADGFWASIVEGPQHRQD
                     ALDGFASAMSAASSVVLARQYASLASVRMDLPLEARAAVLLDCAEMLVSIAPDEALGI
                     VTEVRKRPPPEPLSGEASLLLGMVYAAKADWQRALDIFAALSESRADVIGARALRERA
                     RALEATGDATDALDAALKISYLFPDQVDLAAEGMYNAARLAVARGDSALAAKIGQSLR
                     AAYPDSPWIARLEALAGMPAGASKP"
     gene            complement(6843..7529)
                     /locus_tag="VFH83_04205"
     CDS             complement(6843..7529)
                     /locus_tag="VFH83_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485596.1"
                     /translation="MNVKRFAVLCILAAAALVLASCATSRLQAYHLYGSALSAQLLVP
                     PEPTMDVHYNVTLDANNPVGSVLSVGSNLAKASQATKAEQAMREALTSVDVPGIVLEE
                     AYSTCVSALQAQRHDAVDGSDFRLVVDIRQYGIQARSMSGAVTLHMALTASIVHVMSG
                     DLVWRRGITINDAANPQMFGLGDIVGNMVTASALSEMTPEQLAVGFKNLAMEAGRSVA
                     RSLDRDLNRY"
     gene            complement(7624..9117)
                     /locus_tag="VFH83_04210"
     CDS             complement(7624..9117)
                     /locus_tag="VFH83_04210"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET6485597.1"
                     /translation="MRQKAPRLPSLLVASLMICAIAGAARPGSVRADERLLVDAPGAL
                     WPVWQAFVARHPLPPGVVAQRAAPAEGQGDTPGAAAASGAPRLELRIGPIPGLRVVAR
                     VAAAPTVTLGDPRMSATLAEVRAGKVPVQPVAAIAPPNVAIPVDGEYPGDPEYPLYDE
                     IDASLAGSSGAAQQWLMKLPVVDAAAPDRDTVDWIGAVGDIMPARGVDSTLLSGAGGV
                     ERVFGDTLPVLRSFSLLLGNLEATATGSGPRANKSYTFRFDARAVSTLVSSGFSYLSL
                     TNNHTFDFGSSGFLETLAAVQAAGAATSGVGENQAAAAEPFVTKVGDTEVRILSFADY
                     PVDQTGFDGRKVAMATPDRPGMLWLDSTGLEVASRGFRPGALNIAMVHGGQEWSHVPT
                     PEQRAMYRSLVDAGATVVIGSHPHVLEGLESYHGGLIAYSVGNFLFPGMQGTDGGEQS
                     VILRLGVYQGAVRYVEYTPVRLSGITVRLDRTEATVRSLQAQTRALR"
     gene            9180..>10188
                     /locus_tag="VFH83_04215"
     CDS             9180..>10188
                     /locus_tag="VFH83_04215"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET6485598.1"
                     /translation="MARRIEIFDTTLRDGNKLPFAVLSPDDRLLLANQLARLGVDVLE
                     AGWPGSSAEESACVRRIASEVRGPRIAALARALDSDVEKALEALRDAQRPQIHVYLSV
                     SPQFLAAVLKETEADAIRSLGRCLSTARMAGVRVQCSLSEAPHGRPEFVRDVCVAARE
                     SGAQVINLADTNGILTPERASELVAQVREYLGGRGAAPDGTDAPVIGIHCHNDLGLAT
                     ANSLSAILAGASHVEVTVGGFGERAGNASLEEIAFLISAYGERYGLAHGVHLQEIART
                     SQLLDSLTGIRTHPNKPIIGQLAFVPVAGGFPGKSLDRSLGALMQEKTIGRGSARIAV
                     PI"
BASE COUNT         1745 a         3390 c         3464 g         1589 t
ORIGIN      
        1 ccggcgcgga gagcgaggcg ctccgcaacc agctcgagaa agccgtggag ctggaggatt
       61 acgagcgggc tgccgagctt cgggaccaga tcagaaagat cgagtccaat gtttgagttc
      121 acgggcctct ccgacatggg cgggtggatg cgcgagcgcg gggaacccga ggccgccgcc
      181 gcgggcacca cggaccccag cgcttccgtg gtcatctcca gccggctccg ccttgcgcgc
      241 aacctgaccg ggttcccgta tccggggcga tgcccggcgg acgtggagca gcaggtgcgg
      301 gagcgtatcc tgggcgcgtt tcggcgcttg ccgaacgccg aggattttgc gatcctatcg
      361 ctggacgatc tctcctccct cgagaggcgc atcctcttcg agcggaatct gatctcccaa
      421 gggtactccc tggaaaagca taaggcgctg gttctgagct cggacgagtc cgtggccgcc
      481 acgatcgggg aggaggacca tctccgcctg gtgggtctgg gcaacggagc gtccctggag
      541 aagctcctgg cgcgtctgga cgatctggat tccgccctgg aaccggcgct cccctacgcc
      601 gcagcgtcgg agtggggata cctgaacacg gcgatcacga acctgggcac gggcctcaag
      661 gcttccctca tgctccacct gccggcgctc tccatgagcg ggctgatcaa gagggccgcc
      721 aaggcttcag cccagttggg gctgtccatt cgggggttct tcggcgaggg gtcggagtcc
      781 ctgggagaca tgtatctgat cgcgaacgag gtcaccatcg ggctcacgga gcgggagatc
      841 atcgggaacc tcggtcgaat cgcggtccag ctcgccggat acgagagccg gacgcgagag
      901 gagttgatgg agaagagacg ggtcgacgtg gaggaccggg tctatcgggc ctggggtctt
      961 ctgacctggt gccgatccat aacgctgagg gaggccatcg aggcgctctc ggcgctgcgc
     1021 atgggcgttg ggcttggtct gatcacgacc gtggaggcgt ccaccatcac ggcgctcctg
     1081 ttcctgtccc agaagtcgca tctgcagcgg gtgctgcagt cccacgcgga cacggtggac
     1141 gggaagctga tcgactacac cagggcgaag atcatccgcg aggcgttgac gaaaacgagg
     1201 ggagggtgac gccatgttca agggactgac acaacgggca cagaaggtgc tcaccatcct
     1261 ggcacaggag gaggccaagc gcttccattc ggaccagctg ctgccggagc acatcatcct
     1321 tgcgctgctc aaggacgggg agggggtcgc cgtcaaggcc ctccagcgca tcaaggtcag
     1381 catcgcggag atgcagtcgg agatcgagaa gagcctcccg aaggggaagg gcggcctgat
     1441 cctgggggac gtgccgccgt ccaagcgggg gcagaagatt ctcgaggatt ccgccgaaga
     1501 ggcccgcaac atgggccacg agtacatcgg gacggagcac ctgctcctgg cctccatcaa
     1561 ggagcagggg agcatcgtgg ctcggtacct gtcgcgattc aacgtgacgc cgaacgggct
     1621 gcgggaggcc atcctccaga tcagcggctc cggagagtac aagggctctc ccagcgcggc
     1681 cggcaagaag aaggcgcagg cgcccacgcg caagcccacg ccgaccctgg acgagttcgg
     1741 gcgggacctc acctccttcg cgcgggacaa caagctggat ccggtgatcg gacgggagaa
     1801 ggagatccag agggtcatcc agatcctggc ccgccgcacc aagaacaacc cggtgctcat
     1861 cggcgagccc ggcgtcggaa agacggccat tgttgaggga ctcgcgcaga agatcgtgga
     1921 cgggacggtt ccggaggtgc tgatcggcaa gcgcgtgctg accctggacc tggcttccct
     1981 ggtggctggc acgaagtacc gcggggagtt cgaggagcgc ctgaagaggg tcatgaagga
     2041 gatcctcggc gcgggaaacg tcgtgctctt catcgacgag cttcacacca tcatcggggc
     2101 gggcggggcg gagggcgcca tcgacgcgtc caacatgctg aaaccggcgc tgtcccgcgg
     2161 tgagctgcag tgcatcggcg ccacgaccct caacgagtac aagaagcaca tcgagaagga
     2221 cgccgccctg gagcggcggt tccagccgat ctacgtcagc gagccgtccg tggaagagac
     2281 cacggagatc ctcaagggca tcaagaggaa ctacgaggag caccaccacg tggtgtacac
     2341 gccggcagcc atcgaagccg cctccacgct ctcccgccgc tacatcacgg agcggtacct
     2401 gcccgacaag gccatcgacc tgatcgatga gtccgggtcc cgccggagga tccgcaactc
     2461 catccgcccg cgggagctcg cggatctgga gaaggagatc gaacggctga acctggagaa
     2521 gaccaacctg gtcaacaccc agaactacga gaaagccgcc gcggtccgcg acatggtccg
     2581 ccagctcaag accaaggtgg acgacatcaa gaagagctgg gagagcaccg tcagcgtcga
     2641 agagaacgtg gtggacgtgg aggacatcca gcaggtcatc tccgacatga ccgggatccc
     2701 tttgtcccgg atcgcccaga gcgagagcga gaagctgctg cacatcgagg aggagctgca
     2761 caagaaggtg atcgcgcagg aggaggccat ctcggtcatc gcctccgcca tccgccgatc
     2821 ccgcaccggc ctctcttccg cccgccggcc gttgggctcg ttcatcttcc tgggtcccac
     2881 cggcgtcggg aagaccctgg tggccaagac cctggcggag ttcctctttg gggacgcgga
     2941 tgcgctggtg cgcatcgaca tgtcggattt catggagaag cacaacgtct cccggctggt
     3001 cggggctccc ccagggtatg tcggatacga ggagggcgga ctcctgacgg agaagatccg
     3061 caggcgcccg ttcagcgtga tcctgctgga cgagatcgaa aaggcgcacc cggacgtctt
     3121 caacatcctc ttgcaagtcc tcgaggaggg acagctctcc gacaacctgg gccactccgt
     3181 gagcttccga aacgcggtgt tgatcatgac ctccaacctg ggggcccggg agatcaacaa
     3241 ggactcctcc cttggcttcc agtccgagga gagcgggatg aacttcaagg acatccgcaa
     3301 ctacgtgatg tccgaggtca agcgcgcctt caaccccgag ttcatcaacc gcatcgatga
     3361 gatcgtggtg ttccacaacc tggaccgcac ccatctctcc cagatcttcg acctgatgct
     3421 caaggagatc tcggtccggc tggcggagca gaagatcacg atctccgtaa agaagagggc
     3481 ccgggagtac ctgatcgaga aggggttcga cgagaagttc ggggcccggc cgctgcggcg
     3541 cctgctgcag aaagaggtcg aggacccgct gtccctcgag atcttgaagg gcaagttcaa
     3601 gagcggggac gccattgaga tcgaggtgcg cggggagagg atagcgttcc gcaaggtgga
     3661 gcccaaaaag atcctcgccg ctcccgccca gcccgcgggc ctcctcccgg gggagaaaga
     3721 accggagaag gtcacgacct aggccatgga tggccgaagg agccgtgagg gcagggatgc
     3781 ccgataggct gctagcatga cccgccgcgc ggcctgggtc ctgggcgccc tcctgctcgt
     3841 gaccccgtgc tttgccgaca cggccgcgga cctgcttgcc accggaaaga aggcgttcac
     3901 ggacggcctc tatccggtgg cggctcgctc cttccagcgc atcctcaacg agttccccgg
     3961 gtccgacggg gcaacggaag ccgagtacct cctgggcgtc gcgttcttct actcgggaga
     4021 ttgggcctca gcgcagagca cgcttctggg atttcggacc catcatcccg ggaccgctct
     4081 cgcagaccga accagctact ggctgggcgc ggcctccctg aaacagggga actatccggc
     4141 cgcactgggg tacctggagc aatcgctcgc cccgagcggc tccggaacgc caaacccgta
     4201 tcggctgcag tctctgctgc tcacgggggt cgcccaggag tccctgggcc gggacgcgga
     4261 ggcggctgcc gcctaccggc tcctgatctc ggatcccggc gcgggggggc tcgcgcccga
     4321 agcggtcttc cgcctcgccg gcaccgcgta ccgcgcgggg gacttcgccg gagccagcga
     4381 cctctatggc cgcgtagtgt tgagttaccc gggctccccc ttcgtggcag actccctgtt
     4441 cttcctggga gagagcctgc tttccttggg gaacctcgcg gacgctgagc aacgctacac
     4501 gacgctcctg tcgctttacc ccgattctcc ctaccgcgag gcggcgctgt tccgactcgc
     4561 ggacatcgcc tggcgtggct cccgggcaac ggatgccctt gctcgcctgg acaccctgaa
     4621 ccagcagttc ccggcgggcg cgtaccgcgg cagcgcttac cggctccgcg gtgacatcct
     4681 cctgagtcag caggcctacg acctggcgct gaatcagtat cagggcgcgg tctctctcct
     4741 gcctcagggc acggagcgac agtccgccct gtactccatg gggatcgcgc agctcatgct
     4801 cggcaagcgc gagcaggccg cgcaatcctt caaggcggcg caggtcggcg gatcgtctga
     4861 catcgctcgg aaggcggcgc gccaggccgc cctcctggac gcctcggacg atgccccgga
     4921 tcagggcatt ccatccctgc aatcgtatgt gggcggagag gaccagggtc cgggagccca
     4981 gggccccggc gcgccgatag cccaggccaa cggggatgcg cggaccctgc tggcctcgct
     5041 gcaggagcag aagggggatt acgacggcgc ggcagtcacc tggtcctccc tgatcgagcg
     5101 cgcgccggac ccggcttccc ttccggagta cctgtaccga cgggcacagg accttctccg
     5161 cgccggccga ccctcggcgg ccctcgatga tctccagcgc gtcctgcggg actacccgag
     5221 ctcagcctgg tccgcgcaaa gctcctacgc cgtcggatac gcctactccc tttccagcga
     5281 gtatgccagg gcactgccgt actttcgttc tgcggcaacc ggcgcaggga ccacggcgac
     5341 cgcagcgggc gccgcggacg tggcggcccg aagcaggttg gcggtcggag tctgcctctt
     5401 cgacatggga gactttccgc gcgcgctttc cagtctccag gacctgctgg ccaatctgcc
     5461 tgccggcctt ccccaggcca ccgtcgtgct gtacgtcggg aggaccctct accggatgga
     5521 gcggctcggg gaggcggcgc agaccctggg gcaggcagcg tccctcttcg ccgcctctcc
     5581 gccgcggccg gccggggagg ccggcttcct cgatccggtg gggcaggccg cggacgcggt
     5641 ctactggcag gcctgggcgc tcttccggct cgggaggttt gcggaagcgc gggacgcgtt
     5701 tctgtcgatc ccgacccgat tcccgtcgga cacccgcgcc gccgaggcgc tcttgcgggc
     5761 cggcatctgc gacacgctga gcgccgacga cccggacgcc gtcccgctct tcgaccgagt
     5821 cctggcaagc caggcgtcgg ggatcgatga cttgcgcgag caggcgctgt atgaaaaggg
     5881 ttgggccctc tcccgccagg gcaggtccca ggacagcgcg gccgacttcg agctcctggc
     5941 gcgtcggtac ccggacggcg ccttcgctcc ccaggctttc ctgaagcttg ccagcacagc
     6001 cctggaaaac aagcggtttg ccgacgcgct ggcgggcttc cagcgggtcg cgaaggactt
     6061 cccggaatcg gtggcagcgc cccgcgcccg gtacctgatt gcggagacga ggctcgaatc
     6121 cggcgacgca cagggcgcgg ccgacgggtt ctgggcgtct atcgtggagg gaccccagca
     6181 tcgtcaggac gccctggacg ggttcgcctc cgccatgagt gcggcctcca gcgtcgtgct
     6241 cgcccgccag tacgcctccc tggcatcggt ccggatggat ctcccgctgg aggcccgagc
     6301 cgctgtgctc ctggactgcg cggagatgct cgtctccatt gcgccggacg aggcgctcgg
     6361 gatcgtgacc gaagtgagga agcgtcctcc tcccgagccg ctgtcgggtg aggccagcct
     6421 gctcctgggc atggtgtacg ccgcgaaggc ggactggcag cgcgcgctgg acatcttcgc
     6481 cgcgctgtcg gaatcccgag ctgacgtgat cggggcccgc gccctgcggg aacgggctcg
     6541 tgccctggag gccaccggag atgccaccga tgctctggat gccgctttga agatctccta
     6601 tctcttcccg gaccaggtgg acctggccgc ggaggggatg tacaacgcgg cccgattagc
     6661 cgtggcgcgc ggggattccg cgttggccgc gaagatcggt cagagcctgc gggcggcgta
     6721 ccccgacagc ccgtggatag ccaggctcga agcgctggca ggcatgcccg ccggcgcttc
     6781 gaagccgtga cgacgtccgc tgggggcggg ccgaggggcc gcaacgggca tcgccggtcc
     6841 gcctagtacc ggttgaggtc ccgatccaac gatcgcgcta cggaccgccc ggcctccatg
     6901 gccagattct tgaaccccac ggcgagctgt tccggcgtca tttcggaaag cgcggatgcg
     6961 gtcaccatgt tgccgacgat gtcccccagg ccgaacatct ggggattcgc cgcgtcgttg
     7021 atggtaatgc cgcgccgcca caccaggtct ccgctcatga cgtgaacgat gctcgcggtg
     7081 agagccatgt gcagcgtgac ggcaccggac atcgagcgtg cctggatgcc gtactggcgg
     7141 atgtcgacga ccagtcggaa gtcggagccg tcgacggcgt cgtgccgctg ggcctgcagc
     7201 gcggagacgc aggtggagta ggcctcttcc aggacgatgc ccgggacgtc cacggacgtg
     7261 agcgcctctc tcatcgcctg ctccgctttc gtggcctggc tggccttggc gaggttgctc
     7321 cccacgctga ggacgcttcc taccgggttg ttggcgtcca acgtgacgtt gtagtggaca
     7381 tccatcgtgg gctccggagg caccaggagc tgggccgaca gcgcggagcc gtagagatga
     7441 tatgcttgca gccggctcgt cgcgcaggaa gccaatacca gcgcggcggc cgcgaggatg
     7501 cagagcacgg cgaaccgttt cacattcatg gggaaacctc ctcttctcga cgcctagtat
     7561 aaccccagcg tcctcgggac ggggcactcc ggtcaggcgt ttcactggcc gagaccgcgg
     7621 tcctcagcgt agggcccgag tctgagcctg caaggagcgc accgtggctt ccgttcggtc
     7681 cagccgcacg gtgatcccgg acaggcgcac cggagtgtac tcgacatagc gaacggcccc
     7741 ctggtacacg ccgagacgca ggatgacgga ctgctccccg ccgtccgtcc cttgcatgcc
     7801 cggaaagagg aagttcccga ccgagtaggc gatcagaccc ccgtggtacg actccagccc
     7861 ttccagcacg tgagggtgag atccgatcac cacggtggcc ccggcgtcca ccagcgaccg
     7921 gtacatcgcg cgttgctcgg gggttgggac atggctccac tcctgcccgc cgtgaaccat
     7981 ggcgatgttc agggcgccgg gtcgaaatcc ccgggatgcg acttccaggc cggtcgagtc
     8041 gagccagagc atgccgggcc ggtccggcgt cgccatggct accttccgac catcgaagcc
     8101 cgtctggtcc accggatagt ccgcgaacga gaggattcgc acctccgtgt cccccacctt
     8161 cgtcacgaag ggctccgcgg ccgcggcctg gttttcgccc accccggatg tcgccgcgcc
     8221 ggctgcctgg accgccgcga gcgtctcgag gaatccgctg ctcccgaaat cgaaggtgtg
     8281 attgttggtg agggacaggt aggagaagcc cgagctcacc agggtggaca cggcgcgagc
     8341 gtcgaaccgg aacgtgtagc tcttgttggc ccgagggcca gaacccgtgg ccgtcgcctc
     8401 gaggttgccc aacagcaggg agaacgaccg aagaacggga agcgtgtccc cgaacacccg
     8461 ctccactccc cctgccccgg agagcagggt ggagtcgacc ccacgcgccg gcatgatgtc
     8521 ccctacggca ccgatccagt ccaccgtatc cctatcgggg gccgcggcgt ccacgacggg
     8581 gagcttcatg agccactgct gcgctgcgcc gctggatccc gccagagagg catcgatctc
     8641 gtcgtagagc gggtactccg ggtccccggg gtactcgccg tccacgggga tggcgacgtt
     8701 cggcggggct attgcggcaa ccggctggac cgggaccttg ccggcgcgga cctctgccaa
     8761 ggtcgccgac atccgggggt cccccagcgt caccgtgggc gccgcggcga cgcgcgcaac
     8821 aacccggagt ccggggatcg gcccgatccg aagctcaagc cgaggcgcgc cggaagcggc
     8881 cgccgcgccg ggcgtgtctc cttgcccctc tgcgggcgcg gcgcgctggg caacgacgcc
     8941 cggcgggagt gggtgccgcg ccacgaacgc ctgccagacg ggccacagcg cgcctggcgc
     9001 gtcgaccagc aggcgttcgt ccgcccggac ggagccgggc cgcgccgcgc ccgcaatggc
     9061 gcagatcatc aaggaggcga ccagcaggga cgggagccga ggagcctttt ggcgcatagc
     9121 agggaacaac atacctccgg tcggattgcg cgtgcaatcc ggaagggcta tcatgccgca
     9181 tggctcgaag gatcgagatc ttcgacacca ccctccggga cggcaacaag ctccccttcg
     9241 ccgtgctctc gccggatgac cgtttgctgc tggccaacca gcttgcccgc cttggcgtcg
     9301 acgtgctgga ggcgggttgg cccggctcct cagccgaaga gagcgcctgc gtgcggcgga
     9361 tcgcctccga ggtccgcggc cccaggattg ccgcgctcgc ccgggcgctg gatagcgacg
     9421 tggagaaggc cctggaggcc ctacgggatg cgcaacgtcc gcagatccac gtctacctgt
     9481 ccgtgtctcc tcagttcctc gcggccgtgc tgaaagagac ggaagccgac gccatccgta
     9541 gccttggccg atgcctgagc accgcccgga tggccggggt ccgggtgcag tgctccctca
     9601 gcgaggctcc ccacggccgt cccgagttcg tgcgggacgt atgtgtggct gcccgagagt
     9661 ccggagccca ggtcatcaac ctggcggaca ccaacggcat cctcacgccc gagcgggcgt
     9721 ccgagctggt agcccaggtg cgggaatacc tgggcggccg aggcgctgcc ccggatggga
     9781 cggacgcgcc ggtgatcggc atccattgcc acaacgacct gggccttgcg acggccaact
     9841 cgctctcggc gatcctggcg ggcgcttccc acgtggaagt gaccgtggga ggcttcggcg
     9901 agcgggcggg aaacgcatcc ctggaggaga tcgcctttct catctcggcc tacggcgagc
     9961 gctacggact ggcgcatggc gttcacctgc aggagattgc caggacctcc cagctcctgg
    10021 acagcctgac cggcatacgc acgcacccca acaagccgat catcgggcag cttgcgttcg
    10081 tgcccgtggc cggagggttc ccggggaagt cgttggaccg aagcctgggc gcgctcatgc
    10141 aggagaagac cattggcagg ggatccgctc ggatagccgt gccgataa
//
LOCUS       DASRZS010000172         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1630286, whole genome shotgun sequence.
ACCESSION   DASRZS010000172 DASRZS010000000
VERSION     DASRZS010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630286"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1993)
                     /gene="htpG"
                     /locus_tag="VFH83_04220"
     CDS             complement(134..1993)
                     /gene="htpG"
                     /locus_tag="VFH83_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HET6485599.1"
                     /translation="MAKHQFQAEVNQLLTLIIHSLYSHPEIFLRELVSNASDALDKLK
                     YLTLTQDEYKGMPFAPRIDILYDTKDRKTLTVTDSGIGMNDTDLEENLGTIARSGTRE
                     FIQAMAGDAAKGTSLIGQFGVGFYSAFMVADRVEVVTRKVGDEKAWKWTSDGKGEFDI
                     AEAERDGAGTTVTLFLNDAGREFADRWRIEEIIRKYSNHIAFPIHLHYQEEGQHGGKV
                     DKVSQVNSALALWRRPKGDLKEEDYKEFYKTLTHDDEEPLLWIHLSVEGALAYSTLFY
                     IPKRAPFDLFRYDYQPGVKLYIKRVFITDSEKELLPVYLRFVRGIIDSEDLPLNVSRE
                     ILQKNRVMENIRSSSVRKILQELEHLAKDKTRYGEFWNEFGRVVKEGVVQDSEHRDQL
                     LELLRFKTTGSTGWTSLAEYRDRMQKDQKAIYYITGVREASLRDSPLLETYKNRGLEV
                     LVMDDEIDEIVSGAVGSYLETPLKAVNRSSTADDLKSEQDVGREAAIEPLVKKVKKAL
                     GDLVKDVRASHRLADSPSCVVVDENDPTVQMQQILKLMGNRDFPDFKPILEINPSHPI
                     VTSLAGVEDEDVIADASRLLLDQALLIEGAEIKDPAGFVKRLNRTLEKSLKKG"
     gene            complement(2079..>2391)
                     /locus_tag="VFH83_04225"
     CDS             complement(2079..>2391)
                     /locus_tag="VFH83_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485600.1"
                     /translation="ATDSTLKGIRTSFDQQAVTVTAEVTGALKKAFTDAILRVYFWGM
                     FVVVAGFIVTLFLPELALRKRSGASVVGASEGGAAPSAGPVTPGPAASAPPPTPTSEE
                     A"
BASE COUNT          395 a          776 c          747 g          473 t
ORIGIN      
        1 cctggcgtac gaaagcactc ggacgatgag cgcccagctc accttccgca gagcgccctt
       61 tccaggctcc tcgctccaga gcatgaaccg gttgccgtgc atcaggctcc cccttcgggg
      121 tgagccaccg ggctcagccc ttcttcagag acttctcgag cgtccggttg agccgtttga
      181 cgaaccccgc cggatctttg atctcggcgc cttcgatgag gagcgcctgg tccaggagga
      241 gccggctggc atcggctatg acgtcctcat cctccacccc ggccaggctg gtcacgatgg
      301 gatgggaggg gttgatctcc agaatcggct tgaagtccgg gaagtcgcgg tttcccatga
      361 gcttgaggat ctgctgcatc tgcacggtgg gatcgttctc gtccaccacc acgcacgacg
      421 gtgaatccgc caggcgatgg gaggcccgga cgtccttgac caggtccccg agggccttct
      481 tcaccttctt gaccagcggc tcgatggcgg cctcccggcc cacgtcctgc tcgctcttca
      541 ggtcatccgc ggtagaggac cggttgacgg ccttgagggg ggtttcgagg tagctaccga
      601 cggcgccgga cacgatctcg tcgatctcgt catccatgac caggacttcc agaccccggt
      661 tcttgtaggt ctccaggagg ggagaatcgc gcaggctggc ttcccggacc ccggtaatat
      721 agtagatagc cttctggtcc ttctgcatcc ggtcccgata ttccgccagg ctggtccatc
      781 ccgtggagcc cgtggtcttg aatcgcagga gctcgaggag ctggtcgcgg tgctcggaat
      841 cctgcaccac gccctccttg accaccctcc cgaactcgtt ccagaactcc ccgtagcgcg
      901 tcttgtcctt ggccaggtgc tccagctcct ggaggatctt gcggacggag gaggaacgga
      961 tgttttccat cacccggttc ttctggagga tctcccggct gacgttgagc ggcaggtcct
     1021 ccgagtcgat gattccccgc acgaaccgca gatagacggg caggagctcc ttctcgctgt
     1081 cggtgatgaa tacccgcttg atgtagagct tcacgccggg ctgatagtcg tagcggaaca
     1141 ggtcgaaggg cgcccgcttg gggatataga acagcgtgga gtaggcaagc gcgccttcca
     1201 ccgagaggtg gatccacagg agcggctcct cgtcgtcatg ggtgagggtc ttgtagaact
     1261 ccttgtagtc ctcctccttg agatcgccct tgggtctccg ccacagggca agggcggagt
     1321 tcacctggga gaccttgtcc accttcccgc cgtgctgacc ttcctcctgg tagtggaggt
     1381 ggatggggaa ggcgatgtga ttggagtact tgcggatgat ctcctcgatg cgccaccgat
     1441 cggcgaactc gcggccggcg tcgttgagga acagggtgac cgtggtgccc gccccgtccc
     1501 gctccgcctc cgcgatgtcg aactccccct tgccgtcgct ggtccacttc caggccttct
     1561 cgtctcccac cttccgcgtc accacctcga cccggtccgc aaccatgaag gcggagtaga
     1621 aacccacgcc gaactgcccg atcaggctgg tccccttggc ggcgtccccc gccatggcct
     1681 ggatgaactc ccgcgtgccg gaccgggcga tcgttccgag gttctcctcc agatccgtgt
     1741 cgttcatccc gatgccggag tccgtcaccg tgagcgtctt tcggtccttg gtgtcgtaga
     1801 ggatgtcgat ccgcggagca aagggcatcc ccttgtactc atcctgggtc aaggtaaggt
     1861 acttgagctt gtccagggcg tccgaggcgt tggacaccag ctcgcgcaga aagatctccg
     1921 ggtgcgagta cagggaatga atgatcaggg tgaggagctg attgacctca gcctggaact
     1981 gatgcttcgc catggccgcc tctccgttga acgatatctg ccgcgtgcgt ggatgacccg
     2041 gcaaacgtaa cgagaaatcc gaggcgcgta aaggccgtct atgcttcttc ggaggtcggc
     2101 gtgggcggcg gcgccgaggc cgccggcccg ggcgtgacgg gacccgctga cggcgcggcg
     2161 ccgccctccg aagcccccac cacggaggcg cccgagcgct ttcgaagcgc gagctccggc
     2221 aggaacagcg tcacgatgaa cccggcgacc acgacgaaca tgccccagaa gtagaccctc
     2281 aggatggcgt ccgtgaacgc cttcttgagc gcgcccgtga cctccgcggt caccgtgacc
     2341 gcttgctggt cgaagctggt ccggatgccc ttgagcgtcg aatccgtggc c
//
LOCUS       DASRZS010000173         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_866946, whole genome shotgun sequence.
ACCESSION   DASRZS010000173 DASRZS010000000
VERSION     DASRZS010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866946"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFH83_04230"
     CDS             <1..258
                     /locus_tag="VFH83_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET6485601.1"
                     /translation="YITRRMGEKYKVLATLGKGELIGEMSFFDKSVRSATVIAKGELD
                     ALEFTRENFADIYAASPQWTRRLLVSLARRIHAMVDRLQKT"
     gene            complement(287..733)
                     /locus_tag="VFH83_04235"
     CDS             complement(287..733)
                     /locus_tag="VFH83_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485602.1"
                     /translation="MDRRPSVARLVRERDVYYTIRWSALKKSDKYDIVRSVPSEAGIY
                     EIYYMDDYEKLCLFHVGKSWYGGLRNEIRLRTDQELENDAHRRSILEEYDSYYRWSLI
                     SSSDDMADIIFFYGRTYLPGVAIARGSGRYERIFVKEVDSDKIVTI"
     gene            complement(723..1601)
                     /locus_tag="VFH83_04240"
     CDS             complement(723..1601)
                     /locus_tag="VFH83_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6485603.1"
                     /translation="MTDKASHNGATAAELVGVGLWIGGKPILRGITWQVGPGEKWAVL
                     GLNGSGKTTLLKLLSGYRFPSRGTVRILGETLGSTDVRALRKRVGWVHGDLAPEFPLF
                     MSAAEVVASGTRGVIAVYDDGPEARSAAVRDALRSVAALHLAGRPFHQLSTGERQRVL
                     IARALAARPELLLLDEPCAGLDPVAREGLLRSLEHLFETRPSLTVVSVTHHVEEIVRG
                     YSRVLLLGRGRVAGVGPRDQVMREETLAGIFGPECRVSRRDGRYSLSFRRDGSGSGGR
                     TRTGSARGRRGGNGGS"
     gene            1703..2530
                     /locus_tag="VFH83_04245"
     CDS             1703..2530
                     /locus_tag="VFH83_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HET6485604.1"
                     /translation="MVSVTVRLYEELGGFLPESRRGTPFTVQCREGTTAKALVEDLGV
                     PHTEVDLLLVNGESVGFGRVLEEMDRVSVYPVFEAWDISGVSKVRPVPLRETRFVLDV
                     HLGKLARFLRMMGFDAAYGTASEDDDLVLQARREARILLSRDRGLLKRRAVTHGYLVR
                     STDPRRQLAEVLHRFDLARQLQVLSQGAGIPGQGLHATGAARCAICNAPLVRVSADSV
                     AREIPPQVATRYADFSRCPQCSRVFWRGTHWERMQMLAREALAESTRRSGVACDPNS"
     gene            complement(2520..2723)
                     /gene="rpmE"
                     /locus_tag="VFH83_04250"
     CDS             complement(2520..2723)
                     /gene="rpmE"
                     /locus_tag="VFH83_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HET6485605.1"
                     /translation="MKTETHPRYEYTTITCACGNVIQTKSTAKDIKVEICSKCHPFFT
                     GKQHLVDTAGRVERFKRRYNIKN"
     gene            complement(2766..>3282)
                     /locus_tag="VFH83_04255"
     CDS             complement(2766..>3282)
                     /locus_tag="VFH83_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET6485606.1"
                     /translation="VEHTRDVVILLDSITRLARAYNQTVPTSGKILSGGVDSNALHKP
                     KRFFGAARNIEEGGSLTIVATALIETGSRMDEVIFEEFKGTGNMEVILDRKLADRRLF
                     PAINIKKSGTRKEELLLKEEELQKMWVLRKVINPMEDQEIIELLIDRMRKSKNNDAFL
                     KSMNTSSVTDA"
BASE COUNT          529 a         1083 c         1064 g          606 t
ORIGIN      
        1 tacatcaccc gccggatggg agagaagtac aaggtcctgg ctaccctggg caagggagag
       61 ctcatcgggg agatgagctt cttcgacaag tccgtgcgct cggcgacggt gatcgccaag
      121 ggggagttgg acgcgctgga gttcacccgg gagaacttcg cggacatcta cgcagcgagt
      181 ccccagtgga cccggaggct gctggtgtcc ctggccagac gcatccacgc catggtggat
      241 cgcctgcaga agacctgagg cggccgcccg aactgatcgc ccagccctag atggtgacga
      301 tcttgtccga gtccacttcc ttgacgaaga tccgctcgta gcgccccgac cctcgggcaa
      361 tggcgactcc cggcaggtac gtccgcccgt agaagaaaat gatgtccgcc atgtcgtcgc
      421 tgctgctgat gagggaccat cggtagtagc tgtcgtactc ctccaggatc gagcgccgat
      481 gggcgtcgtt ctccagctcc tggtcagtcc gcaggcggat ctcgtttcgc agcccgccgt
      541 accaggactt cccgacgtga aagaggcaca gcttctcgta gtcgtccata tagtagatct
      601 cgtagattcc cgcttccgag ggcacgcttc gaacgatgtc gtacttgtcg ctcttcttca
      661 gcgcggacca acggatcgtg tagtagacgt cccgttctcg gacgagccgg gcaaccgacg
      721 gcctacgatc caccgttacc ccctcgccgt ccgcgtgccg aaccggtccg tgtccgcccg
      781 ccgcttcccg aaccgtcgcg gcggaagctc agactgtagc ggccgtctcg gcgtgagacc
      841 cggcactccg ggccgaagat gcctgccaac gtttcctcgc gcatgacctg gtcccgaggc
      901 ccgacccccg ctaccctccc ccgacccagg agaaggactc tggaatatcc gcggacgatc
      961 tcctctacgt ggtgcgtcac ggagacgacg gtgagcgagg gcctggtctc gaagaggtgc
     1021 tccagggatc ggaggagccc ctcacgcgcc accgggtcca gcccggcgca cggctcatcg
     1081 aggaggagca gctcgggtct tgccgccaga gctcgcgcaa tgaggacccg ctggcgctct
     1141 cccgtggaaa gctggtggaa gggacggccg gccagatgga gggccgccac cgaccgaaga
     1201 gcgtcccgca ccgcggcgga gcgcgcctcg gggccatcgt catagaccgc gatgacgccc
     1261 cgggtcccac tggccaccac ctccgcggcg ctcatgaaga gcgggaactc gggagccagg
     1321 tccccatgga cccaacccac ccgcttgcgc agcgcgcgga cgtcggtgga gcccagggtc
     1381 tcccccagga ttcggacggt gcctcgggac ggaaagcggt agccggagag cagcttcagg
     1441 agcgtcgtct tcccgctgcc gttcaggccg agcacggccc acttctcccc cggccccacc
     1501 tgccacgtga tgccgcgaag gatgggcttg ccgccgatcc agagacccac gcccaccagc
     1561 tccgccgcgg tagcgccgtt gtgtgaagcc ttgtctgtca tttccggaca cgctcggtgg
     1621 cagcgtacca cgatccggcc aaccgagtca ggccgacgtc ggagcgcttt ccaagccggg
     1681 ctccgttcag tacggttgtc tcatggtgtc ggtgaccgtg cgcctgtacg aggagctggg
     1741 cgggttcctc ccggagagcc gccgcgggac gccattcacc gtccagtgcc gcgagggaac
     1801 cacggcaaag gcgcttgtcg aggatctcgg ggttccccac accgaggtcg atctcctgct
     1861 ggtgaacggg gagtccgtcg gcttcggacg cgtgctggaa gaaatggacc gggtgagcgt
     1921 ctacccggtg ttcgaggcct gggacatttc gggtgtctcc aaggttcggc cggtcccgct
     1981 gcgggagacc aggttcgtcc tcgacgtcca cctggggaag ctggctcggt tcctgcggat
     2041 gatgggattc gacgcggcct atgggaccgc gtcagaggac gacgacctgg ttctccaggc
     2101 gcggcgggag gcgcggatcc tgctcagccg ggatcggggc ctcctcaagc ggagggcggt
     2161 gacccacggc tacctggtcc ggtccaccga cccgcgccgg cagcttgcgg aggtcctgca
     2221 ccgattcgac ctggcgagac agctccaggt tctcagccaa ggggcgggga tccctgggca
     2281 ggggctccac gccaccggcg ccgcccgctg cgccatctgc aacgcgccgc tggtccgggt
     2341 cagcgcggac tccgttgccc gggagatccc gccacaggtg gcgactcgtt acgcagactt
     2401 ttcccgctgc ccccagtgtt cccgcgtctt ctggcgcggg actcactggg agcgtatgca
     2461 gatgctggcc cgtgaggcgc tggcggaatc cacgcgccgc tcaggcgtgg catgcgaccc
     2521 taattcttga tgttgtagcg cctcttgaac cgctcgacgc ggccggcggt gtcaacgagg
     2581 tgctgcttgc ccgtaaagaa cgggtggcac ttggagcaga tttccacctt gatgtccttg
     2641 gcggtggact tcgtctggat gacgttcccg cacgcgcagg tgatcgtcgt gtactcgtat
     2701 ctcggatgag tctcggtctt catggtttcc tccaaagatc cctttcggtc cggcccgagg
     2761 acagctcagg cgtcggttac cgaggacgta ttcatggact tcaggaaggc atcattgttc
     2821 ttgctcttgc gcatcctgtc aatgagcagc tcgatgatct cctggtcctc catggggttg
     2881 atgaccttgc ggagcaccca catcttctgg agctcttcct ctttgagcag gagctcttcc
     2941 ttgcgggtgc ccgacttctt gatgttgatg gcggggaaca gcctccggtc cgccagcttg
     3001 cggtccagga tgacctccat gttgcccgtt cccttgaact cctcgaaaat cacctcgtcc
     3061 attcggctgc ctgtctcgat aagggccgtg gccacgatgg tgaggctgcc accttcctcg
     3121 atgttgcggg cggcgccgaa gaacctcttg ggcttgtgga gcgcgttgga gtccacaccg
     3181 ccggagagga tcttcccgga ggtggggacc gtctggttgt aggcccgcgc caaccgggtg
     3241 atggagtcca gcaggatcac cacgtcccgc gtgtgctcca cc
//
LOCUS       DASRZS010000174         4974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1630808, whole genome shotgun sequence.
ACCESSION   DASRZS010000174 DASRZS010000000
VERSION     DASRZS010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630808"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VFH83_04260"
     CDS             <1..984
                     /locus_tag="VFH83_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485607.1"
                     /translation="EIRNLRTYFRLDEGLLKAVDGVSLSVGRRKTLGVIGESGCGKSV
                     TAQSVMQIVPAPGRIVDGAILLHREDGTVTDLAKLSSHGSEIRAIRGKEISMIFQEPM
                     TSLSPVHTIGNQIMESCLLHITRDRREAKERSIEMLRKVGIPNPGQRFDAYPHQLSGG
                     LRQRAMIAMALSCNPYLLIADEPTTALDVTVQAQILDLMRGLQESLGMAMLYITHDLG
                     VISELADEVAVMYLGQEVEYARVRDIFHQPLHPYTSMLLRSIPKVGLKARTRLEAIAG
                     NVPIPMNLPRECPFLSRCPKAMPGICDRDVPPLTEQAPGHFVRCYLYGAKE"
     gene            988..2013
                     /locus_tag="VFH83_04265"
     CDS             988..2013
                     /locus_tag="VFH83_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6485608.1"
                     /translation="MTDQTLLEVQSLKKYYPVEQGLFRRHVATVKAVDGLDLTINAGE
                     TLGLVGESGCGKTTAGRCILRAIEPTSGKVFLRLNGQMVEVTALNREALYGIRRHAQM
                     IFQDPFSSLDPRMTVLDIISEPLVANGLAHGSEVVDRVLELMKLVGLEAKHLKRYPHA
                     FSGGQRQRIGIARALAPNPQLVVADEPVSALDVSIQAQILNLLQDLQSALKLTYLFVA
                     HDLSVIEHISDRVAVMYVGRLVETAETEELFLRPLHPYTEALLSAVPKPDPDIKMERI
                     ILTGEVANPANPPSGCYFHPRCRYAKDICSKEAPSWQEVRPAHWVSCHFARELRLRGV
                     TEESHTP"
     gene            2035..3573
                     /gene="xylB"
                     /locus_tag="VFH83_04270"
     CDS             2035..3573
                     /gene="xylB"
                     /locus_tag="VFH83_04270"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010269019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HET6485609.1"
                     /translation="MGLLMGIDLGTSSVKALLMDEAGRVLGVSSAEYPILIPEPGAAE
                     QEPQAWWKAAALAVREAIHKSEQPAARIKGIGFSGQMHGMVPLDSAGDVVRPAIIWCD
                     QRSRPQVDRIYKAVGKSKMDRLVLNPLYPGFLLSSLAWMRDREPALFRRLASVLLPKD
                     YLRYRLTGTIGTEESDASSTLAFDVSRRRWAPELVQDLGFDPDCFPPCARSTDVVGTV
                     SAAASADTGLAVGTPVIAGGSDQPMQAVGNGLVAPGAISVTIGTGGQIYSVSEEAVYN
                     PRLNTHTFCNVVPDRWYVMAATLSAGLSLSWFRESVLREKSFETLSREAEGVPPGSEG
                     LLFLPYLAGERTPHLDPDARAVFFGLTLKHTRAHLARAVMEGVALSLRDGYDVLCALG
                     VRVDRIIGSGGGARSALWMQILADVLGRPIHTTQTTEQASFGAAVVAGVGVGVYPSIP
                     DACSRLVRMNRRVTEPIEKNVRLYEKRHGTFRALFESTRGLFASFVETESDGVARSAP
                     GRRT"
     gene            3573..>4974
                     /locus_tag="VFH83_04275"
     CDS             3573..>4974
                     /locus_tag="VFH83_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485610.1"
                     /translation="MASSPAQPVLEQASIRVEPGDLDSTTGCTVTGAVRSVRLSGPER
                     DASTARGGSQNAGAFDGGLLTARVSPPARAPGERRELSFYLRARDPSGRWEAPLLSEY
                     GPSGHLRFAVVATAAAIVFELRTDWRDTPLRMEVPLSLIGGTTWHDIVGRYNGPHAEL
                     LVDGALVDEEWPIGALPGRERDRILIAGREEEGRAAGGFQGLLGTVAVWDRWLADPEV
                     EALCGGAAAVAARGWTLEEPSDRVQYRYDRGGGFVGDCLPFFHEGTYHFYHLFDRRHH
                     GSKFGLGAHQWAHSSSRDLVHWQHHPLAIPITEEREGSVCTGSMFFHDGTWYGFYATR
                     LPDRSEHLSLATSGDGIHFTKTEPNPFASPKPPYRHGPFRDPHVFRDEKTGLFHMMVT
                     AELERPAVPGRGGCLAHLVSRDLRHWEQEEPFLVTGYGDQPECSELYLWRGWYYLVFS
                     HYGLAHYRMARSPFGPW"
BASE COUNT          795 a         1658 c         1669 g          852 t
ORIGIN      
        1 gagatccgaa acctgcggac ctacttccgg ctggacgagg gcctgctcaa ggcggtagac
       61 ggggtcagcc tctcggtggg gcgtcggaag accctcgggg tgatcgggga gagcggctgc
      121 gggaaaagcg tgacggccca gtcggtgatg caaatcgtgc cggcccccgg ccggatcgtg
      181 gacggggcca tcctgctcca tcgggaagac ggcacggtga cggaccttgc gaagctgtcc
      241 tcgcacggga gcgagatccg ggccatccgc ggcaaggaga tctccatgat cttccaggag
      301 ccgatgacca gcctctctcc ggtgcatacg atcggaaacc agatcatgga gtcgtgcctg
      361 ctgcacatca cgcgggaccg aagggaggcc aaggagcggt ccatcgagat gctgcgcaag
      421 gtggggatcc ccaaccctgg ccagcggttc gacgcctatc cgcatcagct ctccggaggg
      481 cttcggcagc gcgccatgat tgccatggcg ctgtcctgca acccgtacct cctgatcgcg
      541 gatgagccga ccaccgccct ggacgtgacc gtgcaggcgc agatcctgga cctcatgcgc
      601 ggtcttcagg agagccttgg catggccatg ctgtacatca cccacgacct cggcgtgatc
      661 tccgagctcg ctgatgaggt ggcggtgatg tacctgggcc aggaggtgga gtacgccagg
      721 gtccgggata tctttcacca gcccctgcac ccctacacct cgatgctgct gcgctccatc
      781 ccgaaggtcg ggctgaaggc ccgcacgcgg ctggaagcga ttgccggaaa cgtcccgatt
      841 cccatgaacc ttccccgcga atgcccgttc ctctctcgat gtccgaaggc gatgcccggc
      901 atctgcgacc gcgatgtgcc tccgctcacc gagcaggcgc ccggccactt cgtgcgctgt
      961 tacctctatg gcgccaagga gtagggaatg accgatcaga ccctcctgga ggtccagtcc
     1021 ctcaagaagt actacccggt cgagcagggc ctgtttcggc gccacgtcgc gaccgtcaag
     1081 gcggtggatg ggctggacct gaccatcaac gccggggaaa ccctgggtct ggtgggagaa
     1141 agcggctgcg gaaagaccac cgcgggtcgc tgcatccttc gtgccatcga gcctacctcc
     1201 ggcaaggtct tccttcgcct caacgggcag atggtggagg tcacggccct gaaccgcgag
     1261 gccctctacg gcatccgacg tcacgcgcag atgatcttcc aggacccttt ctcttccctg
     1321 gacccgcgga tgacggtgct ggacatcatc tccgagcccc tggtggcaaa cggtctggcc
     1381 cacggttccg aggtggtcga ccgggtcctc gagctcatga agctcgtggg attggaagcc
     1441 aagcacctga aacggtatcc ccacgcattc agcggggggc agcggcagcg tatcggtatt
     1501 gcccgcgccc tggcgcccaa tccccagctc gtggtcgcgg atgagccggt gtccgcgctg
     1561 gatgtgtcca tccaggcgca gatcctcaac ctcctgcagg acctgcaatc cgcgctcaag
     1621 ctcacgtacc tcttcgttgc ccacgacctc agcgtgatcg agcacatctc ggatcgcgtc
     1681 gcggtcatgt acgtgggccg gctggtggag accgcggaga ccgaagagct cttcctgcga
     1741 ccgctccacc cttacacgga ggcgctcctc tccgcggttc cgaagccgga tcccgacatc
     1801 aagatggagc gcatcatcct gacgggtgag gtggccaacc ctgctaatcc cccctccggc
     1861 tgctacttcc atccccgctg ccgctacgcc aaggacatct gctccaagga agcgccgtcg
     1921 tggcaggagg tacggccggc ccactgggtg agctgtcact tcgcccgcga gctgcggctt
     1981 cgcggcgtca ccgaagaatc ccataccccg tagctcgacg gatcgggagc gcgcatgggc
     2041 ctgttgatgg gcatcgatct ggggacctcc agcgtgaagg ccctgctcat ggacgaggcg
     2101 ggccgggtgc tcggcgtgtc cagcgccgaa taccccatcc tgatcccgga gccgggggcg
     2161 gcggagcagg agcctcaggc gtggtggaag gccgcggcgc ttgcggttcg ggaagccatc
     2221 cacaagtccg agcagcccgc ggcccggatc aagggcatcg ggttctccgg ccagatgcac
     2281 ggcatggttc ccctggattc cgccggggac gtggtgcggc cggccatcat ctggtgtgac
     2341 cagcgctccc ggccccaggt cgaccggatc tacaaggcgg tgggcaagag caagatggac
     2401 cgcctcgtct tgaacccgct gtatcccggg ttcctgcttt cgtctctggc gtggatgaga
     2461 gaccgggagc cggccctctt ccggcggctg gcctcggtgc tgctcccgaa ggactacctg
     2521 cgctaccgac tgaccggaac catcgggacc gaggagagcg atgcctcctc gacgctggcg
     2581 ttcgacgtat cccggcgccg ctgggctccc gagctggtcc aggacctggg gttcgatccg
     2641 gactgcttcc ctccctgcgc ccgatccacc gatgtcgtcg ggacggtcag cgcggcggcc
     2701 tccgccgaca ccgggcttgc cgtcgggacc ccggtgatcg ccggcggatc cgaccagccc
     2761 atgcaggcgg tcggcaacgg tctggtggcc cccggcgcca tttccgtgac catcgggacc
     2821 ggaggacaga tctactccgt ctcggaagag gccgtgtaca acccgcgctt gaatacccat
     2881 accttctgca acgtcgtgcc ggaccgctgg tacgtgatgg cggccacgct cagcgccggt
     2941 ctgtcgctgt cctggttccg cgagagcgtg cttcgggaga agagcttcga gacgctctcc
     3001 cgtgaggcgg agggggtgcc accgggaagc gagggtttgc tcttcctccc gtaccttgcc
     3061 ggagagcgga cgcctcacct ggatccggat gcccgggcgg tctttttcgg gctcaccttg
     3121 aaacataccc gggctcacct ggcgcgcgcg gtaatggagg gggtagccct gtcgctgcgg
     3181 gacggttacg acgtgttgtg cgccctcggt gtgcgggtgg accggatcat cggatcgggc
     3241 ggaggcgcgc gaagcgcgct gtggatgcag atccttgcgg acgtcctggg tcgcccgatc
     3301 cataccaccc agactacgga gcaggcgagc ttcggagccg ccgtagtggc gggggtgggg
     3361 gtgggcgtgt atcccagcat ccccgacgca tgctcccggt tggtgcgcat gaaccgtcgc
     3421 gtcacggagc ccattgagaa gaacgtccgt ctctacgaga agcgccatgg gacgttccgg
     3481 gccctcttcg aaagcacgag gggcctcttc gcgtcgttcg tggaaaccga atccgatggc
     3541 gtcgcgcgga gcgcgcccgg aaggagaacc tgatggcatc gtcccctgcg cagcctgtcc
     3601 tggagcaagc gtcgatccgg gtggaaccgg gggacctcga ttccaccacg ggatgcaccg
     3661 ttaccggcgc ggtacggagc gttcggcttt ccggccccga acgcgacgcc tccacggcgc
     3721 gaggcgggag ccagaacgcc ggagcgttcg acggaggatt gctgacggca cgggtgagcc
     3781 cgccggcgcg tgccccgggc gagcggcggg agctctcctt ctacctgagg gcacgggatc
     3841 cctcgggtcg ctgggaggcg ccgcttctct cggagtacgg tccgtccggg cacctgcggt
     3901 tcgccgttgt agcgaccgcc gcggccatcg ttttcgagct gcgcacggac tggcgggaca
     3961 cacctctccg catggaggtg ccgctctccc tcatcggagg aacgacctgg cacgacatcg
     4021 ttgggcgcta caacggcccg cacgcggagc tgctggtgga tggcgcgctg gtggacgagg
     4081 agtggcccat tggcgcgctg cccggacgcg agagggaccg gatcctgatt gccggccggg
     4141 aggaggaggg cagagcggcc ggcgggtttc aaggcctgct gggcaccgtg gcggtctggg
     4201 atcgctggct tgccgatccc gaggtcgagg ccctctgcgg cggcgcagcc gcggtggccg
     4261 cgcgggggtg gacgctcgag gagccttcgg accgggtcca gtatcgctac gaccgcggcg
     4321 gcgggttcgt cggtgactgc ctgccgttct tccatgaagg gacctatcac ttctaccacc
     4381 tcttcgaccg gcgccaccac ggcagcaagt tcgggttggg agcccaccag tgggcgcact
     4441 cctccagccg ggacctcgtg cactggcagc accatcccct cgccattccg atcaccgagg
     4501 agcgggaagg atccgtctgc acagggtcca tgttcttcca cgacgggacc tggtacggct
     4561 tctacgctac ccggctgccc gatcgttcgg agcacctcag cctggccacg agcggcgacg
     4621 gcatccactt caccaagacc gagccgaatc cgttcgcctc tcccaagcct ccttaccggc
     4681 acggaccgtt tcgggatcct cacgtctttc gggacgagaa gaccggtctc ttccatatga
     4741 tggttaccgc ggagctcgag cggcccgccg tgcccggccg aggcgggtgc ctggcccacc
     4801 tggtctctcg ggatcttcgc cactgggagc aggaggaacc cttcctggtc accggttacg
     4861 gcgaccagcc cgagtgctcc gagctctatc tctggagagg gtggtactac ctggtgttca
     4921 gccactacgg gctggcgcac taccggatgg cccgaagtcc gttcggaccc tgga
//
LOCUS       DASRZS010000175         5240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3942837, whole genome shotgun sequence.
ACCESSION   DASRZS010000175 DASRZS010000000
VERSION     DASRZS010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3942837"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFH83_04280"
     CDS             <1..600
                     /locus_tag="VFH83_04280"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET6485611.1"
                     /translation="TSGGLWLPPWDTTQFNGLVTGADGTATGRSDTVAATQLRDMVIA
                     AGDSRVAAGAVVEFLFEIQAGSGVGQNLLTARVEDTGAADWYRHVEPWALTIRAIITQ
                     QAGVQILNNVINPDKGEVASLEYRQTSATAGPVTVTVFDLSGDVITVLARVASQGPGD
                     YVAVWDGKNRGGRAVARGIYFLRVVAPGIDETRKVLVVR"
     gene            complement(677..1750)
                     /locus_tag="VFH83_04285"
     CDS             complement(677..1750)
                     /locus_tag="VFH83_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485612.1"
                     /translation="MATLLWKENWDRTRARFNAWWKHEGLILCVTAPRQRQVQPIPHP
                     TRPKDIRDVWLDPHYRLAQAEYEMSWTYYGGESFPYFDTQIGPGNLATFIGSEPGYDS
                     ETVWFNPCIEDPDHHPPLSFRTDNPHWKKQMAIIEKGVTSAAGRYLVGIPDLIENVDI
                     LTSLRGMERLLEDMIDRPEFVEKRVSEINRIYFEVYDLIGSRVKDPWGGTTWAAFRVW
                     GEGRTAKLQCDASAAFSPAMLRRFVVPALREECQWLDHSLYHLDGTQCICHLDELLAI
                     DELDGIEWTPQAGRPDGGSPEWYELYRRILRAGKNVQAVSVKPSEVIPLLDATGPKGM
                     YIMTQASSEAEARKLEERVEAYR"
     gene            complement(1781..2212)
                     /locus_tag="VFH83_04290"
     CDS             complement(1781..2212)
                     /locus_tag="VFH83_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485613.1"
                     /translation="MKTRHWLFLVGLVVLVAWTITGCSLLGVSIDSRISMFFSDLNGD
                     RTKAYLNLDPNSTTYGTVGQTTTVWDSNFPVADEPFSYTITSTAPYSASGVSITISGL
                     AASGGSYLFIFVNRGSTFDNYCISDIRSVAGSTTTSIFGLP"
     gene            complement(2223..3242)
                     /locus_tag="VFH83_04295"
     CDS             complement(2223..3242)
                     /locus_tag="VFH83_04295"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET6485614.1"
                     /translation="MKGALLVSSFLLAACLLQALPTEITYTEGDASERLKSGRARDAA
                     IGDVLNTGDSLKTGVDGRAELDQKGVILKISPNTVFTLLERYQSGSTTSDSVISVALG
                     SVKLHYDKLTGKEPLVRTGGATAGARGTDFSVFAGADGSSLIVVDSGEVTVQAAGGSV
                     NLSPNEGVEVPLGQPPGQKITVHSDQVDYRTWNDGKLASMLADPAAAMGTIESAMAGY
                     IANVKDYAAQFEESDQRLLAEQQKRRDIFNQQGKDVAAKYQSDVVTPLAIQTGYLVLN
                     VRYYSLAALSLRRYVGGRLYVFLKSRYITNLADPAWTGFLDRYQAVLGSFEESIVPHL
                     VEGDI"
     gene            complement(3243..3722)
                     /locus_tag="VFH83_04300"
     CDS             complement(3243..3722)
                     /locus_tag="VFH83_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485615.1"
                     /translation="MSRSPRSRTAIRVLLVVAACAGVLAGCASLQRSVDQGDVREVTD
                     LINSGQAAKLSAMSATPFLLDGEIVALRSDVAALWDNVVKAGFRLSDAGLARSVAVAA
                     DSYREFAGTLEVKAFFDSQVKPGTRILELRTAEGTRVLLLLRDAWFKRTLIGFKGPY"
     gene            complement(3800..4783)
                     /locus_tag="VFH83_04305"
     CDS             complement(3800..4783)
                     /locus_tag="VFH83_04305"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6485616.1"
                     /translation="MNYRLLGKTGMKVSEISFGAWAIGSSWGPVDDRQSLAALHRAVD
                     LGVNFFGTADVYGAGRSERLIGQLLRERKEPLYVATKAGRRLDPHTAERYTPGAIRHF
                     VEDSLRNLRIDALDLVQLHCPPTAVYYQPELFDGMDGLVKAGKIKNYGVSVEKVEEAL
                     KAIEDPGVKTVQLIYNLFRQRPAERFFPIARQRWIGIIVRVPLASGLLSGRMSRSTRF
                     RPDDHRQFNRKGQAFDRGETFAGVDFGAGLDAVEELKGILPPGVTLPQFALRWILMQD
                     AVTCAIPGAKTPQQVEENCAASRVPRLSRKVMGAAARVYDQRVRALVHQRW"
     gene            complement(4793..>5240)
                     /locus_tag="VFH83_04310"
     CDS             complement(4793..>5240)
                     /locus_tag="VFH83_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485617.1"
                     /translation="VHFLDMKNVIHGAGGFAEEPGTASGAGLAESDVSGPIIAIWNSQ
                     QTLRWWHVGLVAAGETLYLGDAITGLSMLSRPTPGKLTRSDIHRYAFFTHVTLMAAQI
                     ALGFLETDALSRGAHDEMLAYTAAHATIGVAIPAVMLFAGLENVLP"
BASE COUNT          906 a         1662 c         1778 g          894 t
ORIGIN      
        1 acgagcggtg ggctgtggct gccgccgtgg gatacgacgc agttcaacgg gctggtgacg
       61 ggggcggacg ggacggcgac gggacggagc gacacggtgg cggcgacgca gctacgggac
      121 atggtgatcg cggctgggga cagtcgggtg gcagcgggag cggtggtgga gtttctcttc
      181 gagatccagg cgggaagcgg ggtgggacag aacctgctga ccgcgcgggt ggaggacacg
      241 ggagcggcgg attggtatcg gcacgtggag ccgtgggcgc tgacgatccg agcgatcatt
      301 acgcagcagg ccggggtgca gatcctgaac aacgtgatca acccggacaa gggggaggtg
      361 gccagcctgg agtatcggca gaccagcgcg acggcggggc cggtgacggt gacggtgttc
      421 gatctgtcgg gggacgtgat cacggtgctg gcgcgggtgg cgtcgcaggg gcctggggac
      481 tacgtggcgg tgtgggacgg gaagaaccgc gggggccggg cggtggcgcg ggggatctac
      541 ttcctacggg tggtggcgcc ggggatcgac gagacgcgca aggtgctggt ggtccgctga
      601 gcgcatcggg ccggtgaccc gacccgcctg agcgacaggt cgggccgggg tccgcggatg
      661 gggccggagt cgcctgctat cggtacgctt ccactctctc ttcgagcttt cgggcttccg
      721 cttcgctgga ggcctgagtc atgatgtaca tgcccttggg cccggtagcg tcgagaagcg
      781 ggatcacctc cgaaggcttc acgctcactg cctggacgtt cttgccggcc cgtagaatcc
      841 gccggtagag ctcgtaccac tcggggctgc cgccgtcggg cctccccgcc tggggagtcc
      901 actcgatgcc gtccagctcg tcgatggcca gaagctcgtc caggtgacag atacattggg
      961 tcccgtccag gtggtagagg gagtggtcca gccactggca ctcctcccgg agcgccggca
     1021 ccacgaagcg tcgaagcatg gcgggagaga aggcggcaga agcgtcacac tggagcttcg
     1081 cggtgcggcc ttccccccag acgcggaacg ccgcccaggt ggtgccgccc cagggatcct
     1141 tgacccggga cccgatgagg tcgtacacct cgaagtagat ccggttgatc tccgaaaccc
     1201 gcttctccac gaactccggc cggtcgatca tgtcttccag gagccgctcc atcccgcgga
     1261 gtgaggtcag gatgtccacg ttctcgatca ggtccgggat ccccaccaga tagcgtcccg
     1321 cggcagaggt gacccccttc tcgatgatcg ccatctgctt cttccagtga gggttgtcgg
     1381 tccgaaagct tagaggcggg tggtggtccg ggtcctcgat gcacgggttg aaccagacgg
     1441 tctcggagtc atatcccggt tcggagccga tgaaggtggc gagattccct ggcccgatct
     1501 gcgtgtcgaa atacggaaag gactctcctc cgtagtaggt ccaggacatc tcgtactcgg
     1561 cctgcgccag gcgatagtgc ggatccagcc agacgtcgcg gatgtccttg ggccgagtgg
     1621 gatgggggat cggctggacc tggcgctggc gcggcgccgt gacgcagagg atgagcccct
     1681 cgtgcttcca ccaggcgttg aacctggccc gagtcctgtc ccaattctct ttccagagca
     1741 gggtagccat gagacctcct gatgggtgcc gcgacgaagc ttagggcaga ccgaatatcg
     1801 acgtggtcgt ggagccggcc accgatcgga tgtcgctgat gcagtaattg tcgaaggtcg
     1861 atccccggtt cacgaagatg aagaggtagg aaccgcccga cgcggcaagg ccggagatcg
     1921 taatgctcac gccggaagcg ctgtagggcg ccgtggaggt gatcgtatac gagaaaggct
     1981 catcggccac ggggaagttg ctgtcccaga cggtagtggt ctgcccgacg gttccatagg
     2041 tggtcgagtt cggatccagg ttcagataag ccttggttcg atccccgttc aggtccgaga
     2101 agaacatcga gatccgtgaa tcgatgctca ctcccagcaa gctgcacccg gtgatggtcc
     2161 atgccaccag gaccacaagc ccgaccagga aaagccagtg tctcgtcttc atcaggttct
     2221 cctcagatgt ctccctcgac caggtgaggg acgatggatt cctcgaacga ccccagcacc
     2281 gcctgatacc gatccaggaa tccggtccat gcgggatccg caaggttcgt gatgtagcgt
     2341 gacttcagaa acacgtagag cctgccgcct acataccgcc gaagggaaag ggcggcaagg
     2401 gaatagtagc ggacattgag gacgaggtat cccgtctgga tggcgagggg cgtaaccacg
     2461 tcactctggt actttgcggc gacatccttg ccctgctggt tgaagatgtc ccgccgcttc
     2521 tgctgttccg caaggagccg ctggtccgac tcctcgaact gcgcggcata gtcctttacg
     2581 ttggcgatgt acccggccat ggcgctctcg atggtgccca tggcagccgc ggggtccgcc
     2641 agcatcgacg ccagcttgcc gtcattccac gtccgatagt ccacctggtc ggaatgcacc
     2701 gtgatcttct ggcccggcgg ctgacccaac ggcacctcga cgccttcatt cggcgaaagg
     2761 ttcacggagc cgccggctgc ctgaacggtg acctcgccgg aatccaccac gatgagcgag
     2821 gagccgtcgg ccccggcgaa cacgctgaag tccgtcccgc gcgccccggc cgtggcgccg
     2881 ccggttcgaa ccagcggctc cttgcccgtc agcttgtcgt agtggagctt cacggagccg
     2941 agcgcgacgg agatcaccga atccgaggtc gtggagccgg actggtagcg ctccaggagt
     3001 gtgaagaccg tgttggggct gatcttgagg atcacccctt tctggtccag ctcggcccgc
     3061 ccgtccacgc cggtcttgag agagtcaccg gtattgagga catctccgat ggccgcatcg
     3121 cgggctctgc cggacttcag cctctccgac gcatccccct ccgtataggt gatctccgta
     3181 gggagcgcct ggagaaggca ggcggccaga aggaacgagg aaaccagaag ggcacccttc
     3241 atctagtacg gccccttgaa cccgatcagc gtccgcttga accatgcatc tcgaaggaga
     3301 agcaacacgc gcgtcccttc cgccgttcgc agctccagga tccgggtacc cggcttcacc
     3361 tgagagtcga agaacgcctt gacctccagg gtcccggcga actcccgata actgtccgcg
     3421 gctacggcga cggaccgcgc caggccggca tcggagaggc ggaagccggc cttgaccacg
     3481 ttgtcccaca gcgcggccac gtcggatcga agcgccacga tctcaccatc caggaggaac
     3541 ggggtagcgc tcatcgcgga gagcttcgcc gcctggcctg agttgatcag gtccgtgact
     3601 tctcggacat cgccctggtc cacggagcgc tgcagggagg cgcagcccgc caggaccccc
     3661 gcgcacgcag caacgaccag aagcacccgg atggcggttc gggaccgcgg gctacgagac
     3721 atcgtttcca ttgtagccgg gttggggatg aaatcaagcg ggcagcgacc cggcccggtc
     3781 cgcccctcgc agtcccccct taccagcgct ggtgcaccag ggcccgcacc cgctggtcgt
     3841 agacccgcgc ggcggcgccc atgacctttc gcgagagccg cggcacgcgg gaggctgcgc
     3901 agttctcctc cacctgctgc ggggtcttgg cgccagggat ggcgcaggtc acggcatcct
     3961 gcatcaggat ccaccgaagc gcgaactgcg ggagcgtcac gcccggcggc aggatccctt
     4021 tgagctcctc cacggcgtcc agcccggcgc cgaagtcgac gcccgcgaac gtctctccgc
     4081 ggtcgaaggc ctggcccttg cggttgaact ggcgatgatc gtccggccgg aacctcgtgg
     4141 accggctcat ccgtccgctg agcagcccgc tggccagcgg cacgcgcaca atgatgccga
     4201 tccaccgctg gcgggcgatg ggaaagaacc gctcggccgg gcgctgacgg aagaggttgt
     4261 agatgagctg gaccgtcttc acgccggggt cctcgatggc cttcagggcc tcttccacct
     4321 tctccacgct gaccccgtag ttcttgatct tgccggcctt caccagcccg tccattccat
     4381 cgaacagctc cggctggtag tacacggcgg taggcgggca gtggagctgt accaggtcca
     4441 aggcgtcgat ccggaggttg cgaagactgt cttccacgaa gtggcgtatg gcccccggcg
     4501 tgtaccgctc cgccgtgtgc gggtccaggc gccttccggc cttggtggcc acgtagagcg
     4561 gctctttccg ctcccgaagg agctggccga tgagccgctc gctcctcccc gcgccgtaga
     4621 cgtccgcggt accgaagaag ttcaccccaa ggtccacggc gcggtgcaac gcggccaagg
     4681 actggcggtc atccaccggt ccccaggagg agccgatggc ccaggcgcca aagctgatct
     4741 ccgacacctt catgccggtc ttgcccagga gccggtagtt catggcagcc tcctatggaa
     4801 gaacgttctc cagacccgcg aagagcatga cggcggggat ggcgacgccg atggtcgcgt
     4861 gggccgccgt gtacgccagc atctcgtcgt gggccccccg ggagagcgcg tccgtctcca
     4921 ggaagcccag cgcgatctgg gcagccatga gcgtgacgtg cgtgaagaag gcgtaccgat
     4981 ggatgtcgct tcgcgtaagc ttgcccggtg tgggcctcga aagcatcgag agtccggtga
     5041 tggcgtctcc caggtagagc gtctccccgg cggcgaccag gcccacgtgc caccagcgca
     5101 gcgtctgctg gctgttccag atggcgatga tgggtccgga gacgtcgctc tcggcgaggc
     5161 cggctcccga cgcggtgcct ggttcctccg cgaaaccgcc cgcgccgtgg atgacgttct
     5221 tcatgtccag gaagtggact
//
LOCUS       DASRZS010000176        11082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2022263, whole genome shotgun sequence.
ACCESSION   DASRZS010000176 DASRZS010000000
VERSION     DASRZS010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11082
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022263"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1007
                     /locus_tag="VFH83_04315"
     CDS             45..1007
                     /locus_tag="VFH83_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6485618.1"
                     /translation="MTDGPALLAVQNLVKHFPSPHGPVRAVDGISFRVQEGETLGLVG
                     ESGCGKTTTGRLLLRLEEPGSGAALFEGRDIFGIPKKGLREYRRAVQMVFQDPFASLD
                     PRMTASEIVVEPLVVYGLCRRRERKDRANELLSMVGLDPSHANRFPHEFSGGQRQRIG
                     IARALAVGPRLVVCDEPVSALDVSIQAQIINLLKDLQQARGLTYIFIAHDLSVVKHIS
                     DRVVVMYLGKVMEIASKRELFARPLHPYTRALLASIPVPDPDYPGESFVLEGDAPSPI
                     RPPSGCRFRTRCPVAFDRCGREEPPLVEVGDGHLAACHRVPPAS"
     gene            1114..2364
                     /locus_tag="VFH83_04320"
     CDS             1114..2364
                     /locus_tag="VFH83_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6485619.1"
                     /translation="MRRTRIAMLLAVALSLTGAIAAQADVTITYWQYFYESKVNLMND
                     LIQKFEAQNPGITVEQVTFPYESYQQKVAAAIPAGEGPDVINLFYGWLPLWVKSGYLQ
                     ALPAFDFSSAYFARSFYPFVAPSVNIGGQNYAVPTAVRTLALIYNKRLFREAGLDPNS
                     PPRTLDELVTDARKLSKYDAQGNLVQSGLLMQPNGQGHVWLREVLFRQFGAVPYSADS
                     RTVTYNSPAGLQAFQWYMDRITKDKVGYPNFSTDDVTAFTAQKAAMNIDGSFRIATLN
                     AVKNLEWGVAELPTYKGIKSNFASFWANAITSGVSGQKLDAAVKFLKFLTSPEVQEVW
                     LQKVGELPATPSLSDEYKTDPVVSVFLKGLAYAHATSYVDEAGQRTILVDAVDQVNLK
                     KADPAAVLKDSAAKEQKLIDDFWK"
     gene            2447..3334
                     /locus_tag="VFH83_04325"
     CDS             2447..3334
                     /locus_tag="VFH83_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485620.1"
                     /translation="MTLAARRAATAYAFLFVPLVFFLGVRFGPTLYMLAMSLTDWGLL
                     RKTLHFVGVANYRGILADPVFLRSLANTAEYTVFGVPIVLVLSLCIALLLDRVSRGSG
                     VYRLIVVLPYVTPVVAVAWVWRWMFQTPPLGVINAMYVAVGLRAHEFLNSPTQALPSI
                     LAVNVWVELGYCTIVFLAGIQTIPASITEAARIDGASGGQILRRIVLPLLLPVTLFLA
                     VMEGIQFLRIFTQVYTMSSQALGGPLDSTKSMALYIYQKAFTNFDMGVAASASLVLFV
                     IILGVTLVQLRLFDRKLSY"
     gene            3345..4169
                     /locus_tag="VFH83_04330"
     CDS             3345..4169
                     /locus_tag="VFH83_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485621.1"
                     /translation="MNRRTPGTASAYLVLTLLSVTMLFPFLWMLLSSFKDATQIYEMR
                     LLPSAPTLSNYLYLFTSRNSRFPQWFLNSAIVGIATTVSVLFFDSLVGYTLAKFRFPG
                     RRVVFLLIISTLMIPTEMLVIPWYAMARALKWVNTYWGVMFPGMISAFGVFLMRQFMG
                     TMPDDLIDAARVDGKGELRIFLTVALPLVTPALAILAIFNFIGNWNAFLWPLIAVARP
                     RMFTLPVGLANFSGEAGSNWHYIMTGATVSMAPLVVVFLIFQKQIVKGIAMTGLKG"
     gene            complement(4175..5422)
                     /locus_tag="VFH83_04335"
     CDS             complement(4175..5422)
                     /locus_tag="VFH83_04335"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HET6485622.1"
                     /translation="MSGAAAARFRVRAVHCDHGASDEEVYRSLRRATDPLDRAWERLG
                     KARRIAIKLNQDWPPDRVVMHAGHRQQLVSDPVARAVLRLLRERTRAELVAVDIGVEG
                     MYAGLTDGSSTLLQDVFHEFDVPFVECSREGTIWTSVPGGGRMFDRYPLPRPVVESDA
                     LVSVQKLKSHQFMGITLCLKNLFALMSIQPAGRPRTYYHHLVRLPYVLADLGRILDPA
                     LNILDGLVTQAGREWGPGDHPRICDTLAAGDHAVATDACGAHLMGHDPAADWLTPPFH
                     RDRNALAVAAEGGLGTVELKEIDFQSEVTAPVGEFFARTVDPNETVVSWRRTTAEQAL
                     FYRDRAPDLRGRYAGQYILLQMGQVRWSGDAADIRESRRVLAGEHPEQALWMKYVEHE
                     DSEGEHYEVYEETLRRMRGMGLA"
     gene            complement(5423..6526)
                     /locus_tag="VFH83_04340"
     CDS             complement(5423..6526)
                     /locus_tag="VFH83_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6485623.1"
                     /translation="MALNVAIVGMGPIGTTHATCYTSNKLAKLVAVCDIVKEKAQSAA
                     EKFGARAFTSEVEMLKAMPEIDVVSVTTSGYDNGSMHFEPAMIALDHGKNVLVEKPIC
                     ADVRDARELVAFASRKKLYLGCDLNHYFTECADRAKKLQKDGGVGELVYCMHKVGFNG
                     SETKYTGPGSPRWQTPYSHVKAFCAHPFSVMRYFCGDVVQIQAFLDRPGVRKSAGDPM
                     LSINSIHMRFINGGVGYLISQRGDAMFGLGGWWSYEHAGTKGTFCIENCVEKLHYWNV
                     GKVKDPGMASPPPETHDTGIKDFGATFPRRINAFLEDVSNKVPVEHIRASGRDALATL
                     EYTFAAMKSYENGGALVTPEPLPPLHGDISVVY"
     gene            6701..7195
                     /locus_tag="VFH83_04345"
     CDS             6701..7195
                     /locus_tag="VFH83_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HET6485624.1"
                     /translation="MTSLFSAPGLLEVVIGRGCLTAYEATHLSSGSYVKVSDRVPGDE
                     VYLFFNSHLVGTGEVCVIDGVLAVRLRRITWNPSPIPFAGDAEELFGVLESRIRLASV
                     PFSLNDLSGIGPYGIISLGKKLTEERVAELTVAGVPVATGVVCFGNDGKWALLIDEVY
                     GRDA"
     gene            7192..7905
                     /locus_tag="VFH83_04350"
     CDS             7192..7905
                     /locus_tag="VFH83_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485625.1"
                     /translation="MRTDILVSGSLVEPPFVKGLNPVVYDWARPDMFSKDQIWALSLL
                     HERYAVSLGESLPVAPASCHVHAVDQQTFGELRASVGTGSACVAAQAEPGSTGVPAPD
                     GRRFFAQSKDSAVVFGLKAAQRIERDARALEAASPFSVILFFAAMGGGFARLLSDATL
                     FEGRFLPLLRDAWKQRVRMPGLVASPVGESGGKASGASDIPDSAMILLVQLEMSDTKE
                     LMTMVYPSDHLRKVLRFLS"
     gene            7926..8627
                     /locus_tag="VFH83_04355"
     CDS             7926..8627
                     /locus_tag="VFH83_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6485626.1"
                     /translation="MAQSESADWFERLPRKTLREAERFLRRVTLRREKALYYQEDDAS
                     AAYCVRSGELRLVKWRPDGASFVLGKGFPGDWLGLAEAVMEGPYLCDAVSVSDSELTM
                     VRSSDLPYLMGIEGLGPSLARELAGGYYPLHDVLESGTPELRIVHHLSRLLARSRDAG
                     HTAGTPLLVTTQEEIAEATGLSRETVNRHLNLLQKDGMIEVLRGKISVTRPGALLPGW
                     PAAPASPGGVSPTKH"
     gene            8845..9960
                     /locus_tag="VFH83_04360"
     CDS             8845..9960
                     /locus_tag="VFH83_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015842603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HET6485627.1"
                     /translation="MKRWFFVLIALVVLLVPSMVFATGTKESAGGKLSVGVILPTKDE
                     ERWTQDQQRFQDAFTKAGYNVQILFSQGDSAKEKANVESLISNGVKAIIICPVDGSAA
                     AAAADEAHKAGVKVISYDRLIRDTASVDYYVTFDSIAVGAAWAQYLVDRAAQNGGKGL
                     HLYLYAGAASDNNAFIFFQGAWGVIQPKIADGTFVIENSSEAVALQGKATLTRDEEAK
                     IIGQVTTNWNPNDAKNIAESNLTAAPDSAKGVCYVLSPNDQTARAIGDAFAADKAVTK
                     YYTTGQDADKVSIQYIIDGKQSMTVLKDVRTLVADAIATATDYLKGMTPPQTNTYNNG
                     KIDVPAKPSAIVTVTKDNVKAAIVDSGYYPASTFTGL"
     gene            10052..>11082
                     /locus_tag="VFH83_04365"
     CDS             10052..>11082
                     /locus_tag="VFH83_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019380229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6485628.1"
                     /translation="MGTPILEMKNITKTFPGVKALSNVSFSVAPQEIHCLVGENGAGK
                     STLMKVLSGVHPHGEYTGDIVFDGKTQVFHGIADSERVGIAIIYQELALVPDMTVYDN
                     IFLGHEISRGITVDLNETIKKAAEMLGKVRLSVSPETPVKDLGVGKQQLVEIAKALSR
                     NVKLLVLDEPTAALNEDDCENLFSILRELKGHGVTCVLISHKLKEVIRIADTVTVLRD
                     GQTICTLDAHKGEVSEAVLIRNMVGRDIDNVYPPRSHTIGTEEVLRVQGWSAYSLKAG
                     RSVLRDVSFNVRRGEIVGFAGLMGSGRTELARSIFGNPDGYRVEGAMFVHGAPVHYHH
                     PHDAIEDGVA"
BASE COUNT         1931 a         3635 c         3553 g         1963 t
ORIGIN      
        1 aacccacggc cgccgagcgc tcgagcctcc gacggaggag cctcatgacc gacggcccgg
       61 cgctcctcgc cgtccagaac ctggtcaagc atttcccttc gccgcacggc ccggttcgcg
      121 ccgtggatgg gatctccttc cgggtgcagg agggagaaac cctcggcctg gtgggggaga
      181 gtggctgcgg aaagacgacc accgggcggc tcctgctgcg gctggaggag ccgggctctg
      241 gggcggcttt gttcgaaggc cgcgacattt tcggcatccc gaagaaaggc ctgcgggagt
      301 atcggcgcgc ggtgcagatg gtcttccagg atcccttcgc gtctctggac ccccgcatga
      361 ccgcctcgga gatcgtggtc gagccgctgg tggtctacgg cctgtgccgc aggcgcgaga
      421 gaaaggaccg ggcgaacgag ctcctttcca tggtagggct ggacccctcc cacgccaatc
      481 gcttcccgca cgagttctca ggcggccagc gccagaggat cgggatcgcg cgagcgctcg
      541 ccgtcggacc gcgcctggta gtgtgcgacg agccggtatc cgccctcgac gtatccatcc
      601 aggcgcagat catcaacctc ttgaaggacc tgcagcaggc ccgggggctc acctacatct
      661 tcatcgccca cgacctctcc gtggtcaagc acatctcgga tagagttgtg gtcatgtacc
      721 tgggcaaggt catggagatc gcctccaagc gggagctctt tgcgcggccg ctccatccct
      781 acacgcgggc gctcctcgcc tccatccccg taccggatcc ggactacccg ggggagtcct
      841 tcgtcctgga aggggacgcg ccgtccccca tccgtccccc ctccgggtgc cgcttccgca
      901 cgcgttgtcc ggtggcgttc gaccgctgcg gtcgggagga gccgccgctg gtggaggtcg
      961 gggacgggca cctggccgcc tgtcatcggg tgccgccggc gtcctgacgc ccacgcgccc
     1021 gctgcggccc tcgttccttg gcggggccgg atggaagcgg taccttagca gcatgccgtt
     1081 cagacccccc ctcggaccca atggaggaaa cccatgagaa ggacgcgaat cgcgatgttg
     1141 cttgccgtcg ccctcagcct gacaggagcc atcgcggctc aggccgacgt cacgatcacc
     1201 tactggcagt acttctacga atccaaagtc aacctgatga acgatctcat ccagaagttc
     1261 gaggcgcaga accccggcat cacggtggag caggtgacgt ttccctacga gagctaccag
     1321 cagaaggtgg ccgcggccat tccggcgggc gaagggccgg acgtcatcaa cctcttctat
     1381 gggtggcttc ccctctgggt gaaatccggg tacctgcagg cgcttcccgc gttcgacttc
     1441 agctccgcat acttcgccag atccttctat ccattcgtgg cgccgtcggt gaacatcgga
     1501 gggcagaact acgcggtgcc aaccgcggtc cgcaccctcg ccctgatcta caacaagagg
     1561 ctcttccggg aagcgggact ggatcccaat agcccgccca ggacgctgga tgagctcgtc
     1621 acggacgcca ggaagctctc caagtacgac gcccagggga acctggtgca gtccgggctc
     1681 ctcatgcagc ccaacggcca gggacacgtg tggctgcggg aggtgctctt ccgccagttc
     1741 ggggcggtcc cctacagcgc ggacagccgc acggtcacct acaactcacc ggccggcctg
     1801 caggcattcc agtggtacat ggaccggatc accaaggaca aggtcggcta cccgaacttc
     1861 tcgacggacg acgtcacggc cttcaccgct cagaaggcgg ccatgaacat cgacgggtcc
     1921 ttccgcatcg cgaccctgaa cgcggtgaag aacctggagt ggggagtggc cgagcttccc
     1981 acgtacaagg ggatcaagtc caacttcgcc tcgttctggg ccaacgccat cacgagcggc
     2041 gtctcgggcc agaagctgga cgcggcggtc aagttcctca agttccttac ctctcccgag
     2101 gtccaggagg tgtggctgca gaaggtcggc gagcttcccg ccacgccgag cctgtccgac
     2161 gagtacaaga cggatcccgt ggtcagcgtg ttcctgaagg ggctggcgta tgcccatgcg
     2221 acctcttacg tggatgaggc gggacagcgg acgatcctcg tcgacgcggt ggaccaggtg
     2281 aacctcaaga aggcggatcc cgccgcggtg ctcaaggatt ccgcagcgaa ggagcagaag
     2341 ctcatcgacg acttctggaa gtaaccgagg cccctgggga cgccggctcc gcggacgccg
     2401 gccccgggcg cggtttccag cgcgagggat ggggaaaggg gaaggcatga cactggccgc
     2461 cagacgcgcc gcgaccgcat acgcgttcct gttcgtgcct ctggtgttct tcctgggagt
     2521 tcgcttcggc cccacgctct acatgctggc gatgagcctc acggactggg gtctccttcg
     2581 caagacactc cacttcgtag gggtcgccaa ctaccgcggg atcctcgccg atcccgtctt
     2641 cctgaggtcc ctcgccaaca ccgccgagta cacggtgttc ggcgtcccga tcgtccttgt
     2701 cctgagcctg tgcatcgccc tcctcctgga ccgggtgtcc cgggggagtg gcgtctatcg
     2761 gctgatcgtt gtcctaccgt acgtgacccc ggtggtcgcc gtcgcgtggg tctggcgatg
     2821 gatgtttcag acgccgcccc tgggcgtcat caacgcgatg tacgtagccg tgggcctccg
     2881 cgcccacgag ttcctcaaca gccctaccca ggcgctcccc tcgatcctgg ccgtcaacgt
     2941 ctgggtcgag ctgggctact gcaccatcgt cttcctggca gggatccaga cgatccctgc
     3001 ctccatcacg gaagcggcac gcatcgacgg cgcatcggga gggcagatcc tgcgccgcat
     3061 cgtccttccg cttctcctgc cggtgaccct cttcctggcc gtgatggagg ggatccagtt
     3121 cctgcggatc ttcacccagg tgtacaccat gtcgtctcag gcgctgggag gaccgctgga
     3181 ctccaccaag tccatggcgc tgtacatcta ccagaaggcc ttcaccaact tcgacatggg
     3241 tgtggcggcc agcgccagcc tcgtgctctt cgtcatcatc ctgggcgtca ccctcgtcca
     3301 gctgagactc ttcgatcgga agctcagcta ctaggaggcc ggcgatgaac cgacgaacgc
     3361 cagggaccgc gtcggcctac ctggtattga cgctcctctc ggtgacgatg ctgttcccct
     3421 tcctctggat gctcctgtcg agcttcaagg acgccacgca gatctacgag atgaggctcc
     3481 tgccgtcggc tcccacgctc tccaactacc tctacctctt cacctcgagg aactcgcggt
     3541 ttccgcagtg gttcctcaac agcgccatcg tcgggatcgc caccaccgta tcggtgctgt
     3601 tcttcgattc cctggtcggg tacacgctcg ccaagttccg gttcccgggg aggagagtcg
     3661 tctttctcct catcatcagc acgctcatga tccccacgga gatgctggtg atcccctggt
     3721 acgccatggc acgggccctc aagtgggtga acacgtactg gggcgtcatg ttcccgggca
     3781 tgatcagcgc gttcggcgtc ttcttgatgc ggcagttcat ggggaccatg ccggacgatc
     3841 tcatcgacgc ggcgcgggtc gacggcaagg gagagcttcg gatcttcctg accgtcgccc
     3901 ttcccctggt cacgccggcc ctggccatcc tggccatctt caacttcatc gggaactgga
     3961 acgcgttcct ctggccgctc attgccgtgg ccaggcccag gatgttcacg ctgcccgtcg
     4021 gacttgcgaa cttctccggg gaagcgggga gcaactggca ctacatcatg acgggcgcga
     4081 cggtctccat ggcgcccctg gtcgtcgtgt ttctcatctt ccagaagcag atcgtcaaag
     4141 ggatcgcgat gaccggattg aaggggtagg gcgctcaagc aaggcccatc cccctcatgc
     4201 gtcgaagcgt ctcttcgtat acctcgtagt gctcgccctc cgagtcctcg tgctccacgt
     4261 acttcatcca gagcgcctgc tccggatgct cgccggccag gacccgtcgg ctctcgcgaa
     4321 tgtccgcggc gtcgccggac catcgtacct gccccatctg cagcaggatg tattgacccg
     4381 cgtatcgccc ccggagatcc ggcgcgcggt cccggtagaa gagcgcctgc tcggcggtgg
     4441 tgcggcgcca gctcaccacc gtttcattcg ggtcgacggt tcgggcaaag aactccccta
     4501 cgggcgccgt cacctccgac tggaagtcga tctccttcag ctccacggtg cccagaccgc
     4561 cctccgccgc cacggccagg gcgttgcggt cccggtggaa cggcggggtc agccagtctg
     4621 ccgccgggtc gtggcccatg aggtgggcgc cacaggcatc cgtggccacg gcgtgatccc
     4681 cggcggccag ggtgtcacag atccgagggt ggtcgccggg cccccactcc cggcctgcct
     4741 gggtcaccag cccgtccagg atgttcagcg cggggtccag gatccgtccc aggtccgcca
     4801 gcacgtaggg caagcgaacc aggtggtggt agtaggtgcg cggccgaccc gcgggctgga
     4861 tcgacatcag ggcaaagagg ttcttcaggc agagcgtgat gcccatgaac tggtggctct
     4921 tgagcttctg cacggatacc agggcgtcgg actccaccac ggggcgggga agcggatacc
     4981 ggtcgaacat ccgaccaccg ccgggaacgg aggtccagat ggtcccctct cgagagcact
     5041 cgacgaaggg cacgtcgaac tcgtgaaaga cgtcctgaag gagcgttgag gagccgtccg
     5101 tcaggccggc gtacatcccc tcgacgccga tgtccaccgc caccagctcc gcgcgcgtcc
     5161 gctcccgaag caggcgcaag accgcgcggg ccaccggatc gcttaccagt tgctgccgat
     5221 gccccgcgtg cattaccacg cggtccggcg gccagtcctg gttgagcttg atggcgatcc
     5281 gacgcgcctt gcccagccgt tcccacgcgc ggtccagcgg atcggtggcg cggcggaggc
     5341 tgcggtagac ctcctcatcg gaggcgccgt gatcgcagtg cacggcgcga acccgaaacc
     5401 gcgccgcggc ggcgccgctc acctagtaga cgacgctgat gtcgccgtgc agcgggggaa
     5461 gcggctcagg ggtcaccagc gccccgccgt tctcgtagga cttcatcgcc gcgaaggtgt
     5521 actccagggt tgccagggca tcccgcccgg aggcgcggat gtgctccacc ggcaccttgt
     5581 tcgacacgtc ctccaggaac gcgttgatgc ggcgagggaa ggtggcgccg aagtccttga
     5641 tcccggtgtc atgggtctcc gggggagggg aggccatacc gggatccttg accttgccga
     5701 cgttccagta gtgcagcttc tccacgcagt tctcgatgca gaaggtcccc ttggttcccg
     5761 cgtgctcgta gctccaccag ccgcccaggc cgaacatggc gtctccccgc tggctgatga
     5821 ggtagcccac gccgccgttg atgaagcgca tgtggatgga gttgatggag agcatcgggt
     5881 caccggcgct ctttcgaacg cccgggcgat ccaggaacgc ctggatctgc accacgtcgc
     5941 cgcagaagta gcgcatcacg gagaaggggt gagcgcagaa ggccttgacg tgggagtacg
     6001 gcgtctgcca tcgaggcgag cccggccccg tgtacttggt ctcggagccg ttgaagccaa
     6061 ccttgtgcat gcagtagacc agctcaccga cgcccccgtc cttctggagt ttcttggccc
     6121 gatccgcgca ttcggtgaag tagtggttca ggtcgcagcc caggtacagc ttcttgcggg
     6181 aggcaaaggc cacgagctcc cgggcgtcac ggacgtccgc acagatcggc ttctcgacca
     6241 ggacgttctt cccgtggtcc agggcgatca tggcgggctc gaagtgcatg gaaccgttgt
     6301 cgtagcccga cgtggtgacg gagacgacgt cgatctcggg catggccttg agcatctcga
     6361 cctcggaggt gaaggcccgg gcgccgaact tctccgcggc ggactgcgcc ttttccttca
     6421 cgatgtcgca gacggcgacc agcttcgcca gcttgttcga cgtgtagcag gtggcatgcg
     6481 tggttccgat cgggcccata cccacgatcg cgacgttcag agccataggg tgtacctcct
     6541 ctgcgctttc aggcagatta gtgcgttcgg ggcctcagag gcaagccgcg ccggaggtct
     6601 ggaccgagtg gatgaaggag ggtgacaggc ttgcggactg tgactgcctt cacacaccgc
     6661 agtcatagac ccgcgacgaa cggcgcgaga tactgctcgc atgacgagcc tgttcagcgc
     6721 accgggcctc ctggaggttg tgatcggcag gggttgcctc actgcctacg aggccaccca
     6781 tctctcctcg gggtcctacg tcaaggtctc cgaccgagtc cccggggacg aggtctacct
     6841 cttcttcaac tcgcacctcg tgggcaccgg tgaggtctgt gtcatcgacg gcgtcctggc
     6901 ggttcggtta cgcaggatca cctggaaccc atctcccatt cctttcgcgg gagacgcgga
     6961 agagctcttc ggggtgctgg agtcgcgcat ccggctggcc tccgtcccgt tctccctgaa
     7021 cgacctgtcg gggatcggcc cttacgggat catctccctc gggaagaagc tcacggagga
     7081 acgggtggcg gagctcacgg tggcgggcgt gcccgtagcg actggagtgg tgtgcttcgg
     7141 aaacgacggg aaatgggccc tcctcatcga cgaagtgtac gggagagatg catgagaacg
     7201 gacatcctgg tctccggttc gctcgtggag cctcccttcg taaaggggct gaatcccgtg
     7261 gtgtacgact gggcccgacc ggacatgttc tccaaggatc agatctgggc gctctcgctg
     7321 ctccatgaac ggtacgccgt gtccctcggg gaatcgcttc cggtcgcgcc ggcctcctgc
     7381 cacgtgcacg ccgtggacca gcagaccttc ggggagctgc gcgcctctgt gggaaccggg
     7441 agcgcctgcg tggcggcgca agccgagccg ggaagcacgg gggtcccggc gccggatggt
     7501 cgtcggttct tcgcgcagag caaggattcc gccgttgttt tcggcctgaa ggcggcgcag
     7561 cggatcgagc gggacgcgcg ggcgctggag gcggcgtcgc ccttctccgt gatcctgttc
     7621 tttgccgcga tgggcggagg gttcgcgagg ctcctgtccg acgccaccct gttcgaggga
     7681 cgcttcctgc cgttgctgcg ggacgcctgg aagcagcggg tgcgcatgcc gggtctggtg
     7741 gcgagtccag tcggggagtc cggcgggaag gccagcgggg cgtccgacat ccccgacagc
     7801 gccatgatcc ttttggtaca actggagatg agcgatacca aggagctgat gacgatggtc
     7861 tacccgtcgg accacctgcg gaaggtcctc cgcttcctga gctagggcgg tcgagctcga
     7921 tgccgatggc gcaatcggaa tcagcggact ggttcgagag gctgcccagg aagacgctcc
     7981 gagaggcgga gcgttttctg cggcgggtaa cgctccgacg ggagaaggcg ctgtactacc
     8041 aggaggacga tgcaagcgcc gcctactgcg ttcggtccgg agagctgcgc ctggtcaagt
     8101 ggcggcccga cggcgcctcc tttgtcctgg gcaaggggtt cccgggcgac tggctgggcc
     8161 ttgcggaggc ggtcatggaa gggccgtacc tgtgcgatgc cgtttcggtc tcggactccg
     8221 agctcaccat ggtccgctct tcagacctgc cgtacctgat gggcatcgag ggcctcgggc
     8281 cctctctggc gagggagctg gccggcggct attatccgct ccacgatgtc ctggagagcg
     8341 gcaccccgga gctgcggatc gtccaccatc tttcacgctt gctagcgcgt agccgcgacg
     8401 cgggccacac cgccggcact ccgctcctgg tcaccacgca ggaggagatc gcggaagcga
     8461 ccgggctgag tcgagaaacc gtcaaccgcc acctgaatct gctgcagaaa gatggtatga
     8521 tagaagtgtt acgaggcaag atctccgtaa cacgccccgg ggctctcctt cccggatggc
     8581 ccgcggcacc ggcttcaccg ggtggtgttt ctccgacgaa acactgactg tatacccggc
     8641 gaaacgctat ggctgcgcat ggcccgtcct ggctcggtac ctgttacgtc cggtaaacac
     8701 ccgccgttcg tcccgaaacc gaccgaaggt tgcgccgcaa acgaagcatc ccaactcatt
     8761 acgaaataat gagttgcgtg ttatcccgat ggtcgggtct cttggcatga cagttgcagt
     8821 cctcctgata aggaggcctc actaatgaag agatggtttt ttgtcctgat cgccctcgtc
     8881 gttctgcttg tcccttccat ggtatttgcc acgggaacca aggagagcgc cggtgggaag
     8941 ctttccgtag gcgtcattct tcctacgaag gatgaggagc gctggacgca ggaccagcag
     9001 cggttccagg acgctttcac gaaggccggc tacaacgtgc agatcctgtt cagccagggc
     9061 gactccgcga aggagaaggc caacgtcgag tctctcatct ccaacggcgt caaagccatc
     9121 atcatctgcc ccgtggacgg ctccgcagcc gccgcagcag cggatgaggc ccacaaggcc
     9181 ggggtcaagg tcatctccta tgaccgcctg atccgcgaca cggcgtccgt cgactactac
     9241 gtgaccttcg acagcatcgc ggtgggagcc gcgtgggcgc aatacctcgt cgacagggcg
     9301 gcgcagaacg gcgggaaggg actccacctg tacctgtacg ccggcgccgc ttccgacaac
     9361 aacgcgttca tcttcttcca gggcgcctgg ggcgtcatcc agcccaagat cgcggacgga
     9421 acgttcgtga tcgagaactc cagcgaagcg gtggcgctcc agggcaaggc caccctgacc
     9481 cgggacgagg aagcgaagat catcggccag gtcaccacga actggaaccc caacgatgcc
     9541 aagaacatcg ccgagagcaa cctgaccgcg gctccggatt cggcgaaggg cgtctgctac
     9601 gtcctctccc cgaacgacca gaccgctcgg gccatcggtg acgcgttcgc cgcggacaag
     9661 gccgtgacca agtactacac gacgggccag gacgcggaca aggtgtcgat ccagtacatc
     9721 atcgacggca agcagtccat gacggttctc aaggacgtgc gcaccctcgt ggccgacgcc
     9781 atcgccactg ccacggacta cctcaagggc atgacgccgc ctcagaccaa cacgtacaac
     9841 aacggaaaga tcgacgtccc tgcgaagccg tccgccatcg tcacggtgac caaggacaac
     9901 gtgaaggccg cgatcgtcga ctccggctac taccccgcga gcaccttcac gggactctag
     9961 acacaacgta tactctggtc cgcgttcgat agaaccaggg gacggcgggc gtcgcgcccg
    10021 ccgtcactct agacggatcg aggggagcag catgggtacg cccattctcg agatgaagaa
    10081 catcacgaag acgttcccgg gggtgaaggc gctgagcaac gtgtccttct ccgtggcgcc
    10141 tcaggagatc cactgcctgg tcggtgagaa cggcgccggc aagtcgaccc tcatgaaggt
    10201 gctgagcggc gtccatcccc acggcgagta caccggagac atcgtcttcg acggaaagac
    10261 ccaggtgttc cacggcatcg cggacagcga gcgggtcggc atcgccatca tctaccagga
    10321 gctggccctc gtccccgaca tgacggtgta cgacaacatc ttcctgggcc acgagatctc
    10381 ccgcgggatc accgtggacc tgaatgagac gatcaagaag gccgccgaga tgctcgggaa
    10441 ggtccgtctc agcgtgagcc ccgagacccc cgtaaaggat ctgggcgtcg ggaagcagca
    10501 gctcgtggag atcgccaagg cattgagccg aaacgtcaag ctgctggttc tggacgagcc
    10561 cacggcggcg ctcaacgagg acgactgcga gaacctcttc tcgatcctga gagagctgaa
    10621 gggccacggc gtgacctgcg tcctcatctc ccacaagctc aaagaggtga tccgcatcgc
    10681 ggacaccgtg accgtcctca gggacggtca gaccatctgc acgctggatg cccacaaggg
    10741 agaggtgagc gaggcggttc tcatccgcaa catggttgga cgggacatcg acaacgtgta
    10801 tcctccccgg tcgcacacca tcgggaccga ggaggtcctg cgtgtccagg gctggagcgc
    10861 ctacagcctg aaggcgggcc gcagcgtcct gcgggacgtt tccttcaacg tccgccgggg
    10921 tgagatcgtg ggattcgccg gcctcatggg gtcgggccgc acggagctgg cgcgaagcat
    10981 cttcggcaat ccggacggct atcgggtgga gggagcgatg ttcgtccatg gggcgcccgt
    11041 ccactaccac catcctcacg acgccatcga ggacggggtg gc
//
LOCUS       DASRZS010000177         6560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5486220, whole genome shotgun sequence.
ACCESSION   DASRZS010000177 DASRZS010000000
VERSION     DASRZS010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5486220"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFH83_04370"
     CDS             complement(<1..416)
                     /locus_tag="VFH83_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485629.1"
                     /translation="MALLLVLALALVPVGAGAQTNGTAPTRAVLGLTGIAESADLVGR
                     VPEAGALQGAVVSLFNRSYGRSIGLAVGPITGVAGTVGLQIDAVGDAVQAITTLSAGT
                     SARSLASTVPFDAPGALVSTITSDLAYLWFSLRSFTT"
     gene            complement(416..1963)
                     /locus_tag="VFH83_04375"
     CDS             complement(416..1963)
                     /locus_tag="VFH83_04375"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET6485630.1"
                     /translation="MRERMLRDLLPGIPVQEEHGSRERRVTGLAYDSREVEPGFLFVA
                     MDGQHTDGHRFIPQAVSRGAAAVVHASPLAEYAPTATYVRVASPRRVLSPIAAAFYGN
                     PSLELGVIGVTGTDGKSSTVWFIAQILARLGYRAGFLSTVSFQVADRMVKNPFRQSTP
                     EASEIHGMLREMVETGRQYAVVEATSHGLSERMNRLADVAFDAAVFTNVTHEHLEFHG
                     TFEQYRSDKANLFRALDRTPSKTGRGGGAPLLRAAVVNADDPSAEYFRASTTVHCSSY
                     GLRARADLSAREIRADITGSRFLMRWEGKEAAARVAIPGPFWVENSLAACLTVSRALG
                     LDPLSLAPEIQGLRSVQGRMDYVDQGQPFALIVDYAHTPGAFEKLFPWIREHTPGRIT
                     VVFGSAGERDREKRRMQGRVASRHCDTLVLTDEDPRGEDRMAILEQIAEGCESKTRGK
                     DLFLVPDRPEAIRFAVSRAGSGDTILLLGKGHEGSIIGPTGPAPWDERAEAEGALREL
                     GYGGAGR"
     gene            2075..2440
                     /gene="queD"
                     /locus_tag="VFH83_04380"
     CDS             2075..2440
                     /gene="queD"
                     /locus_tag="VFH83_04380"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HET6485631.1"
                     /translation="MYTVRIEEGFAAAHFLTRYHGKCERLHGHNYKVFVTASGSTLDE
                     GGMLVDFDILKAALRKVIQEVDHTSLNDHPAFSDGNPSAERIAQFIYQKMAGLVPQAR
                     LAMVEVFETDRNRATYSPD"
     gene            2444..4174
                     /locus_tag="VFH83_04385"
     CDS             2444..4174
                     /locus_tag="VFH83_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET6485632.1"
                     /translation="MELREPYLREQLIAYIGSKRSLLPFLHGVLAPLVERIRESRPTP
                     AGSVSFLDPFAGSGAVARLARVMGLRVHANDWEPYTYVINSCHLGIQPSEARRLFRAS
                     GGMTRALSELNALTRPTRGDRYISRHYAPRSTANADWRTERLFYTTENALRIDAARAE
                     IENRYPGTPDRASAWREKVLLLAPLLYEAATHTNTSGVFKACHRGFGGHGGDALHRIL
                     APIVVRAPVLWEAAPASTIRCLDAARFLREQTGDLCYLDPPYATHQYGSNYFMLNTIA
                     LWDRPPVSAARGADGRLVQKAGIREDWRRTRSAFCYRSTALEAMREVVAAADCRYLVV
                     SYSNEGLIGLEELCDLLSETGELSFTARDNVKYPGGKQSLHRTTRNLELALVVDRCAR
                     GPRARGRASAGHVSAGGLLRHVAMRNLLGRSFDPVRVQEAFPTDRGAILVRAGGRRLR
                     LPMRHFWRMEPGTDELGLEGEELDRFLERLSSCEVRDAREELAIVLDLVREETDRREG
                     GRLRREALRLLRRLAHRKNRQLFVRSLAEVQVHAAREESDDGFLAGLARIQEVASRRG
                     LGTPAEGQRP"
     gene            complement(4162..5151)
                     /gene="hflC"
                     /locus_tag="VFH83_04390"
     CDS             complement(4162..5151)
                     /gene="hflC"
                     /locus_tag="VFH83_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HET6485633.1"
                     /translation="MRKTVLILVLIGVVLLIASLLGPYYIVMEGEQAVVVQFGKIVRV
                     ETGAGLKYKVPFIESVVKYSKKIESWDGDAQRIPTSENQFIWVDTTARWRIVDPKKFY
                     ESVGSMTQAQGRLDDVIDSEVRKIISRNPLSEAVRDSNVISQIKRSNVLESVAGAAES
                     SGALSNISTLAQIQYNDINKGRRALSIEMLNEARTIMPQYGMELVDVIIRQIKYSDEL
                     TQSVFDRMIKERNQIAQAFRSDGEGEKARWLGQTTRELKQIQSDATRKAKEIMAKADA
                     DALAIRNKAYAQDPGFADYWMALQEYQTILPKLGKTMTTDADFYKYLYSRYGR"
     gene            complement(5157..6182)
                     /gene="hflK"
                     /locus_tag="VFH83_04395"
     CDS             complement(5157..6182)
                     /gene="hflK"
                     /locus_tag="VFH83_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HET6485634.1"
                     /translation="MSDGGARRTVRKLTPAILIIAVAVVVGLILVFSSFFVVDQKEEA
                     VVLNLGKFSRMVGPGLHFKAPFGVERNYNVPTQRILKEEFGFRTIRSGVVSEYSNADY
                     SDESVMLTGDLNIVDVEWIIQYQITDARQWLFDVENQEKTIRDISQSAINMLVGDRTI
                     FDVIGAERANIEVQGQQLMNDLYKSYGLGIRVTTVKLQNIVPPKGPVQDAFEDVNKAI
                     QDRSRLINEGKEAYNQAIPKASGQADQTIQEAQGYATERVNQARGDVSRFLAVLGEYR
                     QAPDVVRTRLYYEMYDTVFGTSDGTDLVDKNLKNFLPLKSLGSGGAATVPAPAAPAAP
                     AAQGGSQ"
     gene            complement(6278..>6560)
                     /locus_tag="VFH83_04400"
     CDS             complement(6278..>6560)
                     /locus_tag="VFH83_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6485635.1"
                     /translation="EAQTGREAVEKASQVSLDVVVMDMRMPDLGGIEATRAIHDRFPA
                     VRVIGFSALEDPAQVAAMREAGAVGYVVKSDPPGRLLATIRACFEPAGA"
BASE COUNT         1053 a         2209 c         2164 g         1134 t
ORIGIN      
        1 gtggtgaagg agcgtagaga gaaccagagg taggcgagat ccgaggtgat ggtcgacacg
       61 agcgcgccgg gggcgtcgaa gggtacggtg gacgcgaggg atcgcgccga cgtgcccgcg
      121 ctcagcgtgg tgatcgcttg aaccgcgtcg ccaaccgcgt cgatctgaag gccgaccgtg
      181 cccgccacgc cggtgatcgg cccaacggca aggccgatcg atcggccata ggatcgattg
      241 aacagggaaa ccaccgcgcc ctgcagggca ccggcttctg gaaccctgcc gaccaggtcc
      301 gctgactccg cgatcccggt gaggccaagg acggctcggg tgggcgcggt gccgtttgtc
      361 tgggctccgg ccccgacggg caccagcgcc agcgccaaca ccagaagcag ggccatcagc
      421 gcccggcccc tccgtacccc agctcgcgga gcgcaccttc cgcctcggcg cgctcgtccc
      481 agggcgcggg gcccgtcggt ccgatgatgc tgccttcgtg tccctttccc agcagaagga
      541 tggtgtcacc gcttcccgcg cgggagacgg cgaaacggat ggcctcgggc cggtccggta
      601 ccaggaagag gtcctttccc cgggtcttgc tttcgcagcc ctcggcgatc tgctccagga
      661 tggccatccg gtcctcaccc ctggggtcct catccgtcag gaccagggtg tcgcagtggc
      721 gggacgccac ccggccctgc atccttctct tctcgcggtc ccgttcccca gcggaaccga
      781 agaccaccgt gatccttccg ggggtgtgct cgcggatcca ggggaaaagc ttctcgaagg
      841 ctcccggggt atgggcgtaa tccacgatga gggcgaaggg ctgaccctga tccacgtagt
      901 ccatccgacc ctggacggag cgaagcccct ggatctccgg cgcgagggaa agcggatcca
      961 ggcccagagc tcgagagacg gtcaggcacg ccgcgaggga gttctcgacc cagaacggtc
     1021 cggggatggc aacccgcgcc gccgcctcct ttccttccca ccgcatgagg aaccggctgc
     1081 cggtgatgtc cgcccggatc tcccgcgcgc tcaggtccgc gcgcgcccgc aagccgtagg
     1141 acgagcaatg cacggtggtg ctggcccgga agtactccgc gctgggatcg tccgcgttca
     1201 cgaccgcggc gcgcagaagg ggagcgcctc ccccgcggcc cgtcttggag ggcgtacgat
     1261 cgagggcccg aaagaggttg gccttgtccg agcggtattg ctcgaaggtc ccatggaact
     1321 ccaggtgctc gtgggtgacg ttggtgaaca ccgcggcgtc gaacgccacg tcggcgagcc
     1381 ggttcatccg ttccgagagc ccatgggagg tggcctcgac caccgcgtac tggcggcccg
     1441 tctccaccat ctcccgaagc atgccgtgga tctcgctggc ttccggtgtg gactggcgga
     1501 acgggttctt caccatgcgg tccgccacct ggaacgatac ggtggaaagg aagcccgccc
     1561 ggtagcccaa ccgcgccagg atctgggcga tgaaccacac cgtggagctc ttcccgtccg
     1621 tcccggtcac gccgatgacc ccgagctcca acgacgggtt cccgtaaaac gcggcggcga
     1681 tgggggagag gacccgccgg gggctggcca cccgcacgta cgtcgccgtc ggcgcgtatt
     1741 ccgcgagcgg gctggcgtgc acgacggcgg cggcgccacg gctcaccgcc tgagggatga
     1801 accgatgacc gtcggtatgc tggccgtcca tggccacgaa caggaacccc ggttcgactt
     1861 cgcgggagtc gtaggccagt cccgtgaccc tccgctccct ggacccgtgc tcctcctgga
     1921 ccggaatccc cggcaagaga tcgcgaagca tcctctcgcg catcgatcga tgctaaccgc
     1981 gcggagaggg gctttcaaga ccgccgcttc acggtgatac cgccgctccc gcgcgattgt
     2041 ttcccgcccc gggaatcgat atgctggcca ggtcatgtac acggtccgca tcgaggaagg
     2101 cttcgcggct gcccacttcc tgacccgcta tcacgggaag tgcgagcgcc tccacggcca
     2161 caactacaag gtcttcgtga ccgccagcgg ctcgaccctg gatgagggag ggatgctcgt
     2221 ggacttcgac atcctcaaag ccgccctgcg gaaggtgatc caggaggtgg accacaccag
     2281 cctcaacgac catcccgcgt tctccgacgg caaccccagc gcggagcgca tcgcgcagtt
     2341 catctaccag aagatggcgg ggctggtgcc ccaggcgcgg ctcgcaatgg tggaggtgtt
     2401 cgagacggac cgcaaccggg ccacgtattc cccagactga gggatggagc ttcgcgagcc
     2461 ttatctcaga gaacagctca tcgcctacat cggcagcaag cggtccctgc tcccgttcct
     2521 gcacggcgtg ctcgcgccgc tcgtagagcg catccgggaa tcccgcccga caccggcggg
     2581 ctcggtgagc ttcctggatc cctttgccgg cagtggggcg gttgcccggc tggctcgcgt
     2641 catgggcctg cgggtccacg cgaacgactg ggagccctat acctacgtca tcaactcctg
     2701 ccacctcggc atccagccga gtgaggcacg gcgcctgttc cgcgcaagcg gaggcatgac
     2761 ccgcgcgctc tccgagctga acgcgctgac gcggcccacc cggggagacc ggtacatcag
     2821 ccgacactat gctccccgaa gcacggccaa cgcggactgg cggacggaga ggctcttcta
     2881 caccacggag aatgcgcttc ggatcgatgc cgcgcgcgcc gagatcgaaa accgataccc
     2941 ggggacaccg gaccgagcga gcgcctggcg cgagaaggtt cttctcctgg cgccgctcct
     3001 gtacgaggcg gcgacccaca ccaacacctc cggggtgttc aaggcctgtc atcggggctt
     3061 cggcggccac ggaggagacg ccctgcaccg gatcctcgcg cccattgtcg tgcgcgcgcc
     3121 ggtcttgtgg gaagccgcgc cggcctccac gatccggtgc ctggatgcgg cccgcttcct
     3181 gcgggagcag accggcgacc tctgctacct cgatccgccg tacgccacgc accagtacgg
     3241 cagcaactac ttcatgctca acaccatcgc gctctgggac cgaccgccgg tcagcgccgc
     3301 gcgcggggct gacggccggc tggttcagaa ggccggcatc cgcgaggact ggcgccggac
     3361 ccgctccgcc ttctgctatc gttccacggc tctggaggcc atgcgggagg tggtggccgc
     3421 tgcggactgc cgctacctcg tggtgagcta cagcaacgaa gggctcatcg ggctcgagga
     3481 gctctgcgac cttctgagtg agaccgggga gctatccttc accgcccggg acaacgtgaa
     3541 gtatcccggt ggaaagcaga gtctccatcg cacgacacgg aacctggagc tggcgctcgt
     3601 ggtggaccga tgcgcccggg gcccgcgggc ccgtggccga gcctcggccg gccacgtctc
     3661 ggctggcggc ctgcttcggc acgtcgccat gcgaaacctt ctgggccgct ccttcgaccc
     3721 ggtccgcgtg caagaagcct tccccacgga tcgaggcgcc atcctggtcc gggcgggcgg
     3781 tcgccggctc cggctgccca tgcggcactt ctggcgcatg gagcccggca cggatgagct
     3841 cgggctggaa ggggaggagc tggaccggtt cctggagcgt ctgtcctcct gtgaggtccg
     3901 agatgcccgg gaggagctgg ccatcgttct ggatcttgtc cgtgaggaaa cggatcgtcg
     3961 ggagggaggc aggcttcggc gagaggcgct gcggctcctc aggagactgg cgcaccgcaa
     4021 gaaccggcag ctcttcgttc ggagcctggc ggaggtccag gtccatgccg cgcgcgagga
     4081 atccgacgac ggattcctcg cggggctcgc ccgcatccag gaggtcgcct cccggcgcgg
     4141 gttggggacg cctgccgagg gtcagcgtcc gtagcggctg tacaggtact tgtagaagtc
     4201 cgcgtccgtg gtcatggtct tgccgagctt cggcagaatg gtctggtact cctgcaaggc
     4261 catccagtag tccgcgaagc cggggtcctg ggcgtatgcc ttgttgcgga tggccagcgc
     4321 gtccgcgtcg gccttggcca tgatctcctt ggccttgcgg gttgcgtccg actggatctg
     4381 cttcagctcc cgggtggtct ggccgagcca ccgcgccttc tcgccctcac cgtcggatcg
     4441 gaaggcctgg gcgatctggt ttctctcctt gatcatccgg tcgaagacgc tctgggtgag
     4501 ctcatcggag tacttgatct gccggatgat cacgtccacc agctccatcc cgtactgcgg
     4561 catgatggtc cgcgcctcgt tcagcatctc gatggacagc gcccggcggc ccttgttgat
     4621 gtcgttgtac tggatctgcg ccagcgtgga gatgttgctc agggcaccgg agctctccgc
     4681 ggcgccggcc acgctctcca gcacgttgga gcgcttgatc tggctgatca cgttggagtc
     4741 ccgcaccgcc tcgctcagcg ggttgcggga gatgatcttt cggacttcgg agtcgatgac
     4801 atcgtccagc cgcccctgag cctgggtcat ggatcccacc gactcgtaga acttcttggg
     4861 gtcgacgatg cgccagcgcg cggtggtatc cacccagatg aactggttct ccgaggtggg
     4921 gatgcgctgc gcgtctccgt cccacgactc gatcttcttg gagtacttca cgacgctctc
     4981 gatgaagggg accttgtact tgaggccggc gcccgtctcc acgcgcacga tcttgccgaa
     5041 ctgcacgacg acggcctgct ccccttccat cacgatgtag taggggccga gcagagaggc
     5101 gatcagcagg actactccga tcagtaccag gatgagaacg gtcttcctca tggggttcac
     5161 tgggagcctc cttgggccgc gggcgcggcg ggcgcggctg gcgccgggac ggtggcagcc
     5221 ccaccgctgc cgaggctctt cagcggcaga aagttcttca ggttcttgtc caccaggtcc
     5281 gttccatccg aggtgccgaa cacggtgtcg tacatctcat agtagaggcg ggtgcggacc
     5341 acgtccgggg cctggcggta ctctccgagc acggccagga accgggagac atctcccctg
     5401 gcctggttca cgcgctccgt ggcatagcct tgcgcctcct ggatggtctg gtcggcctga
     5461 ccgctggcct tggggatggc ctggttgtag gcctccttgc cctcgttgat cagccggctt
     5521 cggtcctgga tggccttgtt gacgtcctcg aaggcgtcct gcacgggacc cttgggcggc
     5581 acgatgttct ggagcttgac cgtggtcacc cggatcccca acccatagga cttgtacagg
     5641 tcgttcatga gctgctgacc ctgcacctcg atgttggctc gctccgcgcc gatgacgtcg
     5701 aagatggtcc ggtcccccac cagcatgttg atggcggact gggagatgtc acggatcgtc
     5761 ttctcctggt tctcgacgtc gaacagccac tggcgggcgt ccgtgatctg gtactggatg
     5821 atccactcca cgtccacgat gttcagatcc ccggtgagca tgacggattc gtcggaatag
     5881 tccgcgttgg agtactccga gaccacgccg gatcggatgg tccgaaaccc gaactcctcc
     5941 ttcagaatcc gttgggtggg gacgttgtag ttcctctcca cgccgaaggg cgccttgaag
     6001 tgcagacccg gtcccaccat ccgggagaac ttgcccaggt tgaggacgac ggcctcttcc
     6061 ttctggtcca cgacaaagaa gctggagaac acgaggatca agcctacgac cacggccacc
     6121 gcgatgatca ggatggccgg ggtgagcttg cggacggttc tccgcgcacc accatcactc
     6181 atgacacctc cttttggtcc ttgaggatcg aatgcactat agcacgggaa cggtcgtcgg
     6241 tgcgctattg ggcagtccgc gctggagcgg ctcagcctca agcgccggcg ggctcgaagc
     6301 aggcccggat ggtggcgagg agtcggccgg gaggatcgct cttcaccacg tagccgacgg
     6361 cgcccgcctc cctcatcgcg gccacctgcg ccgggtcctc cagcgccgaa aacccgatga
     6421 cccggacggc ggggaaccgg tcgtggatgg ctcgggtcgc ctcgatccca ccgaggtccg
     6481 gcatccgcat atccatgacg acgacatcca gcgacacctg ggaggctttc tccacggctt
     6541 cccgaccggt ctgcgcctcc
//
LOCUS       DASRZS010000178         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_484447, whole genome shotgun sequence.
ACCESSION   DASRZS010000178 DASRZS010000000
VERSION     DASRZS010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484447"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="VFH83_04405"
     CDS             <1..1280
                     /locus_tag="VFH83_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET6485636.1"
                     /translation="WAPDGSLYLVSDRTGWWNLYRWEESAARIAPLYPLEAEFGRPQW
                     RFGVSTYGFVSDRELVSSYIQNGQAHLISLNTRTLQTRPVELPYTEILTLHTGGASAP
                     KAVPSPVLLLGGSPTIPTELARLDPASGRREVIRRSRSLSLPAAWFSVAEPVEFPTDS
                     GKTAHGFFYPPGNPDFAAPRGERPPLLVMSHGGPTGSTSRILRYGIQYWTSRGIAVLD
                     VDYGGSTGYGRAYRERLKGQWGIVDVADCVNGARWLVDRGLVDGRRLAITGGSAGGYT
                     TLCALTFHDTFTAGASYYGVSDLEVLARDTHKFESRYLDGLVGPYPERKDLYLARSPI
                     HHTDRLARPMILLQGLEDRVVPPNQAQMMYDAVRARGLPVAYLTFPGEQHGFVKAENN
                     QRAVEAELYFYSRVFGFDLAEKVEPVAIENLPG"
     gene            1313..2464
                     /locus_tag="VFH83_04410"
     CDS             1313..2464
                     /locus_tag="VFH83_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485637.1"
                     /translation="MAPTSSRDRVLQALAFRPTDYVPCCFSAFSALRQKCASQREFVE
                     RQLAMGLDVVVDFGELPVAHHPSVRNRQWREEVPGSPYPVLHSQYTTPAGTLSRTVHT
                     SADWEHGNEVPFLSDFNIARSSKQLVSGADSLEALAYLLAMPTEAEKKAYAERAAASR
                     RLAAEKGLVAMAGVSQHGDFACWLSGIQELALMAYDHPDFLSRYLALIERWNRERLAV
                     VLSQKPDILVRRGWYENADFWSPDIYRRFLLPGLRRDAEQAHAAGARLGYIVSCSSMP
                     LLDSFLEAGVDVLMGVDPAQDRMMDLRVLKQRTQGRMALWGGVCGYLTMEKGSAEDIR
                     RQVRNALSVLGPGSGLLLAPVTNVRADTPTTRRNVEAMVDEWKRVRTAG"
     gene            complement(2510..>2714)
                     /locus_tag="VFH83_04415"
     CDS             complement(2510..>2714)
                     /locus_tag="VFH83_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485638.1"
                     /translation="VTGLLTDTCFGIYVFHTPVLVGASMLLRSVTLYPLAKALVAGAC
                     TWVITLVVAWLVRRIPTIGKLFA"
BASE COUNT          440 a          931 c          908 g          435 t
ORIGIN      
        1 agtgggcgcc cgatggcagc ctctatctcg tctcggaccg caccggctgg tggaacctgt
       61 accgctggga agagtccgct gcccggatcg ctcctctata ccccctggag gccgagttcg
      121 gacggccgca gtggcgcttc ggggtgtcca cctatggctt cgtctcggac cgcgagctgg
      181 tgagctccta catccagaac ggacaggctc acctgatcag cctgaatacc cgcacgttgc
      241 agactcgtcc cgtcgagctt ccctataccg agatcctcac cctccacacc ggtggtgcct
      301 cggcgccgaa ggcggtcccc agcccggtcc tgctcctggg cggatcgccg accatcccga
      361 ccgagctggc ccggctcgat cccgcctcgg gacgacggga agtcattcga cggagtcgtt
      421 ccctctccct ccccgccgcc tggttctccg tggcggagcc ggtggagttc ccgaccgaca
      481 gcgggaagac cgcgcacggc ttcttctatc cccctgggaa cccggacttc gccgctcctc
      541 gaggcgaacg gccgcccctg ctggtgatga gccacggcgg cccgaccggc tccacctcgc
      601 gcatcctccg ctacggcatc cagtactgga ccagccgcgg gatcgccgtc ctggacgtgg
      661 actacggcgg cagcacgggc tacggccggg cgtaccgcga acgtctgaag ggtcagtggg
      721 gcatcgtgga cgtggcggat tgcgtcaacg gcgcccgctg gctggtggac cgcggcctgg
      781 tggacggcag acgcctggcg atcacgggtg gcagcgccgg tggctacacg acgttgtgcg
      841 cgctcacctt ccacgacacg ttcaccgccg gggcgagcta ctatggtgtg agcgacctgg
      901 aggtcctggc gcgggacacc cacaagttcg agtcccggta cctcgacgga ctggtagggc
      961 cgtaccccga gcggaaggat ctctacctcg cgcgctcccc gatccatcac accgatcgcc
     1021 tggctcggcc gatgatcctg ctccagggac tggaggatcg cgtcgtcccg ccgaatcagg
     1081 cccagatgat gtacgacgcg gtgcgcgccc gtggtctgcc cgtggcgtac ctgacgttcc
     1141 ccggcgagca gcatgggttc gtgaaggccg agaacaacca gcgtgccgtg gaggcggagc
     1201 tgtacttcta ctcccgcgtg ttcgggttcg atctcgccga gaaggtagag ccggtggcga
     1261 tcgagaacct gccgggttga ccgaccgtcc gcgggggacg taccctccgc cgatggcacc
     1321 cacgagctcc cgagatcgcg tcctccaggc cctggcgttt cggcccaccg actacgtccc
     1381 ctgctgtttc agcgcctttt ccgccctgag acagaagtgc gccagccagc gggagttcgt
     1441 ggagcggcag ctcgccatgg gtctggacgt ggtggtcgat ttcggggagc tcccggtggc
     1501 ccatcacccc agtgtccgaa accgacagtg gcgggaggag gtccccggtt cgccctaccc
     1561 cgtgctccac agccagtaca ccacgccggc cgggaccctg agccggaccg tgcataccag
     1621 cgcggactgg gaacatggga acgaggtgcc gttcctgtcc gatttcaaca tcgcccgctc
     1681 ttcgaagcag ctggtgagcg gcgcagacag cctggaggcg ctcgcgtacc tgctggccat
     1741 gcccacggag gcggagaaga aggcctacgc cgagcgggcc gctgcctcgc gtcgacttgc
     1801 cgcggagaaa gggctggtgg cgatggcggg cgtgagccaa cacggagact tcgcgtgctg
     1861 gctctccgga atccaggagc tcgcgctcat ggcgtacgac catcctgatt tcctctcgcg
     1921 ctacctcgcc ctgatcgagc ggtggaaccg cgagcggctg gccgtggtgc tctcccagaa
     1981 accggacatc ctggtgagaa ggggctggta tgagaacgcc gacttctggt ccccggacat
     2041 ctatcggcgg ttccttctgc cggggctgag gcgggacgcg gagcaggccc acgcggcggg
     2101 cgcgcgtctg ggctacatcg tgagctgctc ctccatgccc ctcctggact ccttcctgga
     2161 agccggcgtg gacgtcctca tgggagtgga tcccgcccag gaccggatga tggacctgcg
     2221 agtcctgaag cagaggaccc agggtcgtat ggcgctctgg ggcggggtat gcggctacct
     2281 cacgatggag aagggaagcg ccgaggacat ccgccgccag gtccgcaacg ctctgtcggt
     2341 cctgggtccc ggaagcggac tgctccttgc gcccgtcacc aatgtccgag cggacacgcc
     2401 gaccacccgg cgcaacgtcg aagcgatggt cgacgagtgg aagagagtcc gaaccgcggg
     2461 ctgatcggct ggggggacct ccccacagcc gacccggacg ggaccaggct caggcgaaga
     2521 gctttccgat cgtcgggatc cgtcgcacca gccaggccac gaccagcgta atcacccagg
     2581 tacaagcgcc ggccaccagc gccttggcca gcggatagag cgtcacggaa cggagcagca
     2641 tgctcgcgcc caccagcacg ggcgtgtgga acacgtagat cccgaagcag gtgtccgtca
     2701 ggaggcccgt gacg
//
LOCUS       DASRZS010000179        11735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5487223, whole genome shotgun sequence.
ACCESSION   DASRZS010000179 DASRZS010000000
VERSION     DASRZS010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11735
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5487223"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1887)
                     /locus_tag="VFH83_04420"
     CDS             complement(127..1887)
                     /locus_tag="VFH83_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET6485639.1"
                     /translation="MQHSWLLPLVVLLPWIGALAVLATGRAVRLGTGLAIAVAAADAV
                     LSIVLIPMATSVTAFTIPIGGVFGDFSFSPDGLGVFLAAVAAVIGCLAVIFSVDYMRG
                     EENLSRYYSLILFFIGAMAGLVLSTNFLMLVVFWEITALCSYALISFHNDDPKAVAGG
                     IKALIVTGLGGVGLLAGAFLIYAGLGTFDIPTFLARASTLRPALLAWAGFGFLAAAAA
                     KSAQFPFQTWLPDAMEAPTPISALIHAATMVNAGVYLLARFYPAFSPVPGWTTAVIAV
                     GVLSALLAGVMALASTDLKRVLAYSTVSQLGFMVYAIGTGGIFASQFHLLSHAVFKAL
                     LFLGAGAVIHSLGTRDMREMGGLSKTMPLTASVFILGAFALAGIPFVNGFWSKELLLE
                     GGLANGPLWAYVIMLVTAGLTALYTVRCVWMVFLAPPAREREHVHDAGPAMQVALIPL
                     AVGTLTTWLLAGPFQRLLVSTLPFHFPGGGEAQTSWRIFGEILSAPATYLALGVVALG
                     LLAWLLRKPLAGALRRMERARQGAESGLGFESINRGISRAIQGAGEGLRVTQSGLLNW
                     NVAAILMAIVAVLIAVAWGG"
     gene            complement(1891..2205)
                     /locus_tag="VFH83_04425"
     CDS             complement(1891..2205)
                     /locus_tag="VFH83_04425"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HET6485640.1"
                     /translation="MSGISVWTVGLVAVFALVAIGLYGLLIVRNLIKIVVALQIFAKG
                     TILVMVVAGGAQGQPAIGSSLAITVIVADTVVTVVALALAMQVKRVHGTLDARVISSL
                     KR"
     gene            complement(2202..2705)
                     /locus_tag="VFH83_04430"
     CDS             complement(2202..2705)
                     /locus_tag="VFH83_04430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET6485641.1"
                     /translation="MILQTLVLALMLACAILAIAAKRLLISALWLAGCSALLAVSLSL
                     MGAPEVGVIELSVGAGLVTVIFVFAISIAGEEPVVHASLVPRPLAWVLVAAFLALLAW
                     LTLPWVLSPQTPVASLPVIGAVWQVRALDTLLQVVLIFCGVLGSLGLLSDPRKREPER
                     HTTGGAP"
     gene            complement(2868..3599)
                     /locus_tag="VFH83_04435"
     CDS             complement(2868..3599)
                     /locus_tag="VFH83_04435"
                     /inference="COORDINATES: protein motif:HMM:NF040399.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HET6485642.1"
                     /translation="MNDGLLRFARYAFPPNRLGYCGTDDNAALLGYLSESRSDGGLLD
                     LARKFEGAYPYLRLIAATNGLQDPFDDRVVQAYWIGNELLEKVGASALYRSLKERFKT
                     RMDSSAFQWMTAGLADGTTPHHNFHVLEVYRRAGLMRDDRAAVALDRMDQCRISWGRV
                     TAVEGGEAIVERSPLELRDGKLGLGRPVPVRVTRRLGAGEDGVVPGRIVSIHWSWICD
                     ALDARGLHRLQRETFRAVHHTNATL"
     gene            complement(3596..4534)
                     /locus_tag="VFH83_04440"
     CDS             complement(3596..4534)
                     /locus_tag="VFH83_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6485643.1"
                     /translation="MERGSPGPGATPTSPDPRRGYILALLTMAISGVSIYVNSLGVRL
                     FSDSTLYTALKNAVVGAALLLPLAFSPRVRQAYRRLSRTEWTLLLLVALIGGSVPYAL
                     YFRGLQLSTPVTASLIDHTQFLFVAALAALFLRERFRITVWVALVVLLVGLTLGISVR
                     AVRLDAGVPLLGTATLLFAVDFVVMKQLLRSVDALTVMTFKMTLGSVLLFAFVAATGH
                     AAMVPALSARQLAFVAVTGLILLAFTVSSVLGLRHASATAVTAIPAGSPVITTALVVT
                     AQHAEIPPWRLLGLLLALVSVLAVFLLGRYQERRAA"
     gene            complement(4573..6345)
                     /locus_tag="VFH83_04445"
     CDS             complement(4573..6345)
                     /locus_tag="VFH83_04445"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6485644.1"
                     /translation="MNQQTILMIGSDPQHHSLLRALLTYGRFRVLEAESAEAGIRAAR
                     EVRPDCLLMSLSLCEEDAGAAVRSIREDEALKAIPLIAVSPEPVDGGERKALNAGCDG
                     YFAQPVDAKSFLDNILQFLSVAEARRASERPLRWRGRVLVADSDAQIMRLLADLLRGE
                     GYEASEAAGGERLLEAVSARSPDLVLLDAEMSGLDGFEVTRRIKANLGTLQIPVILMS
                     YPRGGGRWTRGLEAGADEFLVKPVDPAELLVRVQSMIRLKRYAEQLAQFARESQLGPP
                     LAADLQAEPAQPTVMLLGPEASESGPFEQALEERGCDTVRAANEVDALSTAAQRAVDV
                     ILLRVPWPGAGPLGLCRLLKEQDSMRNAQVIVLCPRNDPQSRIAALEHGADDYLTVPC
                     DLAELGTRVLLLARRKSHLDALQARYRSALSAASMDGLTGLYNNTAFKRLLDLEVKRS
                     RRHSISVALILLDVDDFKRVNDGLGHLAGDTVLAEIGAILRQAGREIDLPARYGGDEF
                     AIGLPHTDTRGAMVVAERARKAILTHGFLGEGGAADPVTVSIGVASFPADADDLEELI
                     RATDACLYNAKLKGKNRTCAPDGN"
     gene            complement(6342..8423)
                     /locus_tag="VFH83_04450"
     CDS             complement(6342..8423)
                     /locus_tag="VFH83_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HET6485645.1"
                     /translation="MEQPWYRKAWRRAVLDMHIPDDDERFLTRYDGRQWAELVARSGA
                     ASAVVYAMGHVGLALYPSNVIPPHKGLGGRDLFAEGVDGCHERGIAVSAYLSVIFDTW
                     AYRHNPGWKMIGVDGLAVAEQSRYGTCCPNSPYRDYIAAFTRELCERYSFEGIRFDMT
                     FWPHVCYCPHCRARFQNEVGGALPTRVNWEDPTWVAFQRKREQWLAELAELLTRTARQ
                     AKPGVSVEHQASTYPLNWKFGVSERLVPANDFLQGDFYGDAPSGSIARKLFSNMSPQR
                     PAAFETSIATNLANYTAMKPLELVRTKAHAALADATAFVFIDSVDPLGTLNPAVYHRM
                     ARVFQETRVYEPYLGGQRVEDVVVYLGLTSKCDLADNGKRVDDPTLSTRMPHVESVAA
                     VASVLFAHHVPFGIITRRNLAELSRWRTIVVPNAILMDDEEAAAIREFVRAGGNLYAS
                     RFTSLIGIDGRRRPDFLLADVFGSHYGGETAERFTYMAPTRDGEGLFGDWTAEHPMGI
                     GTRQITVRAEADVTILATLTLPFTNPDDPIHFASIHNNPPGVALKSPSLLEHRFGKGR
                     AIWAAGDIELAEGAEGVTFRLLSRLAEPFTVQTDAPRVVEITTFWQEDRARFLLSMVN
                     FPKELPAVPVLGTQVALHIGARRPRRVLVLPEEKPLPFQVADGFVRFGAPRLDTFLMI
                     AVDCETGERVP"
     gene            complement(8439..11594)
                     /locus_tag="VFH83_04455"
     CDS             complement(8439..11594)
                     /locus_tag="VFH83_04455"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET6485646.1"
                     /translation="MERSLENDFAKPGPWFRGQPFWAWNGALEPQELRRQIRLMKRMG
                     MGGFFMHSRIGLSTPYLRDEWFRSIEVCIDEAQKQNMLAWLYDDDRWPSGAAGGLVTR
                     NPRYRKRSLLMEELKAPAQLRWTAEVVAAFRSTVKGTTASGVQPIPRGRRPSSLSHGE
                     RILSFRVLVDPVSSWYNDATYLDTLNPAAVRRFIAVTHEEYRRHVGRHFGKTLPGIFT
                     DEPNHGQKFDRDNNTGSPLDLPWTEGLPAAFKKRYGYDLIPHLVELYLDVDGQQVTPA
                     RHDFHDCLTALFVDSFSRQIGAWCEEHGLMHTGHVLMEDRLSSQACVVGSAMRFYEPM
                     QAPGIDLLTERWRAYATAKQVSSVAHQLGKRWRISESYGATGWDFPFVGHKAIFDWQF
                     ALGINLRCQHLAWYTMRGEAKRDYPAAISAQSPWWELYPVVEDYFSRVATLLSRGEEV
                     RDLLVIHPVESTWTMIRRGWLAEKEVQDYDRVLVDLEDTLLGAHLDFDYGDEEMLSRL
                     AAVSRRKGTPLLVVGRACYAAVVVPPLRTIRSSTLMLLAEFQAAGGLVVFTGEPPRYV
                     DAAASDAPADLAAQCVAAPPAGPQLAAAVDKAARRVSVTDAEDRELASILYQLREDKD
                     AYYLFLCNTSLTDEDRGRDIFDTGRISEHTLAFPNVTVRARLACDGEPLELDPSTGEI
                     FQADSLRAEDGAWQIATSFPANGSRLFLFPRKPEAQKRERRPPLVDHRRIPLEDGRWT
                     ILRSEANVLVLDRPSYRVDGGSWQGPEEVLRVDGALRAAVGVPPRGGAMVQPWARKRT
                     RSPRRVEVELHYRFEVEAIPTGELSLCVEDPSRYAISLNGAEICSDGDNGFWVDPSLR
                     RLPVDPALLLHGENTLTLRGPYDEEHPGLEIVYLLGEFGVEVKDTEVFMIDAPRSLAL
                     GSWIHQGLPFYSGSVCYCRRVAIERSPEERIVVEVPEYRGVAARVLVDGRPAGVTAWD
                     PPEVDITSLLPEAPGSVEIRIEVIGHRRNSHGPLHHAASWPAWTGPAEFTTKGELWKD
                     GYSLVPCGLLAQPEVVARRPG"
BASE COUNT         2059 a         3951 c         3946 g         1779 t
ORIGIN      
        1 cgggtgctgg cggggatgcc gctcccggag tagccgtcca tgagacgctg gaccacgcgg
       61 ccgatgatgt agatgagcgg cgacgaggcg atcgggagga ctaccagata cagccagggt
      121 tgcatctcat cctccccatg ccacggcgat cagcacggcc acgatggcca tgaggatcgc
      181 cgcaacgttc cagttcagga gtcccgactg ggtgacccga agcccttccc ccgctccctg
      241 gatggcgcgg gagatccctc ggttgatgct ctcgaatccc agcccggatt ccgctccctg
      301 ccgggcgcgt tccattcgtc tcagcgctcc ggccagtggc ttgcggagca gccacgccag
      361 gagccccagg gcgaccacgc ccagcgccag ataagtggcc ggcgcggaga gaatctcccc
      421 gaagatccgc caggatgtct gggcctctcc tccgccgggg aagtggaacg gcagcgtgct
      481 gacgagcagc cgctggaacg gaccggccag gagccacgtg gtgagcgtgc cgaccgcgag
      541 cgggatgagc gcgacttgca tggccgggcc tgcgtcatgc acgtgctcgc gctcccgggc
      601 cggcggtgcc aggaacacca tccagacgca acgaacggtg tacagcgccg taagcccggc
      661 cgtaaccagc atgatcacgt acgcccacag cggcccattg gcgagcccgc cttccagcag
      721 gagctccttg ctccagaacc cgttcacgaa ggggattccg gcaagcgcga atgctcccag
      781 gatgaagacc gaggcggtca gaggcatggt cttggagagt cctcccatct cccgcatgtc
      841 tcgggtgccg agggagtgaa tgacggcgcc ggcgcccagg aagagcagcg ccttgaacac
      901 ggcgtggctg aggaggtgga actggctggc gaagatcccg ccggtgccga tggcgtacac
      961 catgaaaccg agctggctga cggtggagta ggccaacacg cgcttcagat cggtcgatgc
     1021 cagcgccatc acgccggcca gcagcgccga aagcaccccc acggcgatca cggccgtggt
     1081 ccagcccggc acggggctga acgccgggta gaaccgggcc agcaggtaga ctccggcgtt
     1141 caccatggtg gcggcgtgga tgagcgcgga gatcggcgtc ggagcctcca tggcgtccgg
     1201 cagccaggtc tgaaagggga actgcgcgct cttcgccgcc gccgccgcca ggaacccgaa
     1261 gcctgcccac gccaggaggg ccggccggag cgtggatgcc cgggccagga acgtggggat
     1321 gtcgaaggtg cccagaccgg catagatcag gaacgcgccc gccaggagcc cgacgccgcc
     1381 caggcccgta acgatcagcg ccttgatgcc gcccgccacc gccttggggt catcgttgtg
     1441 gaaggagatc agggcgtagg agcacagcgc ggtgatctcc cagaagacca ccagcatcag
     1501 gaaattggtt gagaggacca ggcccgccat ggccccgatg aagaagagga tcagggagta
     1561 gtagcgggag aggttttcct ccccgcgcat gtagtccacc gagaagatca ccgccagaca
     1621 gccaatgacg gccgccaccg cggccaggaa gacacccagc ccatccgggg agaagctgaa
     1681 atcgccgaac actccgccga tggggatggt gaacgcggtg acgctggtgg ccatgggaat
     1741 gaggacgatg ctgaggacgg catccgccgc ggcaacggcg atggccaggc cggtgcccag
     1801 ccgaacggcg cgcccggttg ccagcaccgc cagggcgccg atccagggaa ggaggacgac
     1861 cagggggagc agccaggagt gttgcatggg ctacctctta aggctggaga tgacccgggc
     1921 gtccagcgtt ccatggacgc gcttcacctg catggccagg gccagggcta caaccgtgac
     1981 caccgtatcc gccacgatca ccgtgatggc caggctggag ccaatggccg gctgcccctg
     2041 cgcgccgccc gccaccacca tcaccaggat cgtgcccttg gcgaagatct ggagagcaac
     2101 cacgatcttg atgaggttgc ggacgatcag aagcccgtag agcccgatgg cgaccagggc
     2161 gaacacggca accagaccca cggtccagac ggagatcccg ctcatggcgc acctcccgtc
     2221 gtgtggcgtt ccggctctcg cttccgcgga tccgagagca ggccgaggga gccgaggacg
     2281 ccgcagaaga tgagaaccac ctgaaggagg gtatccagcg cgcgcacctg ccacacggcg
     2341 ccgatcaccg gcagggaagc caccggcgtc tgcggggaca ggacccatgg cagcgtgagc
     2401 cacgccagca gcgccaggaa cgcggctacg agcacccagg cgagtggtcg ggggaccagg
     2461 ctcgcgtgga ccacgggctc ttcaccggcg atgctgatgg cgaacacgaa gatgacggtg
     2521 acgagccccg cgcccacgga gagctcgatc acccccactt cgggggcgcc catcagcgag
     2581 agactgaccg ccaggagtgc gctgcagcct gcgagccaca gcgctgagat gaggagccgt
     2641 tttgcggcga tcgccaggat cgcacacgcg agcatcaggg caaggacgag agtctgtagg
     2701 atcatacaga aaccctcctc acgagaacgt ctggcgtact gggcggctac ccgcagcgcg
     2761 ctatcgttcg atgcctgact atgttgtgcc agcggctaca gattgtgaat tgacgtcatt
     2821 atagcacggg cgctaccgct gtcaaggaaa accttggcgt gcggaagcta cagcgtggcg
     2881 ttggtgtgat ggacggctcg gaacgtctcc cgctgcagac ggtgcagtcc tcgcgcgtcg
     2941 agcgcgtcgc agatccagct ccagtggatc gagacgatcc ggcccggcac tacgccatct
     3001 tccccggctc caagccgccg cgtgacccgc accgggaccg ggcgccccaa ccccagcttc
     3061 ccgtcgcgaa gctcgagcgg ggatcgttcg acgatcgctt ctcctccctc gaccgccgtc
     3121 actcgacccc agctgatgcg gcactgatcc atccgatcca gggccaccgc cgcgcgatcg
     3181 tcccgcatga gacccgccct gcggtacacc tccaacacgt ggaagttgtg gtgaggtgtg
     3241 gtcccgtccg ccaggccggc ggtcatccac tggaacgcgc tcgagtccat gcgcgtcttg
     3301 aaccgctcct tcagagagcg gtacagcgca gaggcgccga ccttctccag cagctcgttt
     3361 ccgatccagt acgcctggac cacgcggtcg tcgaacggat cctgcaaacc gttggtcgcg
     3421 gcgatcaacc gcagataagg ataggccccc tcgaacttcc tcgcaagatc gagcagcccg
     3481 ccgtcggatc gactctcgct gaggtatccc agcagcgcgg cgttgtcgtc cgtcccgcaa
     3541 tagccgagcc ggttgggcgg gaacgcatag cgcgcgaagc gcaggaggcc gtcgttcatg
     3601 cggcccgccg ctcctggtaa cgaccgagga ggaatacggc cagcaccgaa acgagcgcga
     3661 ggaggagccc gagcagccgc cacgggggga tctccgcatg ctgggcggtc actaccagcg
     3721 cggtggtgat gacgggcgag ccggcgggaa tggcggtgac cgccgtagcg gaagcgtggc
     3781 ggagcccgag aacggagctg acggtaaagg ccaggaggat caggccggtg accgccacga
     3841 acgcgagctg ccgcgcggag agcgccggca ccatcgcggc gtgccccgtg gcggctacga
     3901 acgcgaagag cagcacggat cccagcgtca tcttgaaggt catcacggtg agggcgtcta
     3961 cggaccgcag gagttgcttc atgaccacga agtccaccgc gaagaggagc gtggccgtcc
     4021 cgagcagcgg cactcccgca tccagcctca cggcgcgaac gctgatcccg agagtgagcc
     4081 cgaccaggag cacgaccagc gcgacccaca cggtgatccg aaagcgctcc ctcaggaaca
     4141 gcgccgcgag cgcggccacg aacaggaact gggtgtggtc gatgagggag gcggtcaccg
     4201 gggtgctgag ctggagcccc cgaaagtaca gggcataggg cacgctcccg ccgatgagcg
     4261 cgaccaggag caggagcgtc cactcggtcc gacttagccg ccggtacgcc tggcgaacgc
     4321 gcggggagaa cgccagcggg aggaggagcg ccgcccccac gaccgcgttt ttcaaagcgg
     4381 tgtacagcgt ggagtccgag aaaagccgga cgccaaggct gttgacgtag atcgagaccc
     4441 cgctgatcgc catggtcagc agggcaagga tatagcccct tcgaggatca ggactcgtcg
     4501 gcgtagcgcc cggacccggg gatccgcgtt ccatgggtcc aaagataacg atcgaggagg
     4561 acggtcgcct gcctaattgc catccggcgc gcaggttcgg ttcttgccct tcagcttggc
     4621 attgtacaga caggcgtccg tcgcgcggat cagctcttcc aggtcgtccg cgtccgcggg
     4681 gaagctcgcc accccgatgc taacggtcac cgggtccgcc gctcctccct cgcccaggaa
     4741 accgtgggtg aggatggcct ttcgagcccg ctccgccacc accatggcgc cccgggtatc
     4801 cgtatgcggc agcccgatgg cgaactcgtc gcctccatag cgcgccggca ggtcgatctc
     4861 gcggccggcc tgccgaagga tggcaccgat ctccgccagc accgtgtcgc ccgccaggtg
     4921 cccgagaccg tcgttgacgc gcttgaagtc gtccacgtcc agcaggatca gcgccacgct
     4981 gatggagtga cggcgggacc tcttcacctc caggtccagg aggcgtttga acgcggtgtt
     5041 gttgtacagc cccgtgagcc cgtccatgga cgcggcgctg agcgccgagc ggtaccgggc
     5101 ctgcagggcg tccaggtgcg acttccttcg cgccagcagc aggacccggg tgcccagctc
     5161 cgccaggtcg cagggcacgg tcaggtagtc gtcggcgcca tgctccaggg cggcgatgcg
     5221 actctgaggg tcattgcgag ggcatagcac gatgacctgg gcattccgca tcgagtcctg
     5281 ctccttgagc agccggcaga ggcccagcgg ccccgcgccc ggccacggca cgcgaagcag
     5341 gatcacatcc acggcgcgct gggccgcggt ggacagcgca tccacctcgt tggccgcccg
     5401 aaccgtgtcg caccctcgct cctccagggc ctgctcgaaa ggaccggact cgctggcctc
     5461 cggaccgagg agcatcaccg tgggctgcgc gggctccgcc tggaggtccg cggccagcgg
     5521 cgggccgagc tgactctccc gggcgaactg ggccagttgc tccgcgtacc tcttgaggcg
     5581 gatcatggac tggacccgga ccaggagctc ggccgggtcc acgggcttca ccaggaactc
     5641 gtccgcgccc gcctcaaggc cccgcgtcca gcggccgccg cctcgggggt aggacatgag
     5701 gatgacgggg atctgcaagg tgccaaggtt ggccttgatc ctgcgcgtca cttcgaatcc
     5761 atcgagcccg ctcatctccg catccagcag gacgagatcg ggggaccggg cggagacggc
     5821 ttccaggagc ctctcgcctc ccgcagcctc cgaggcctca tatccttctc ctcgaaggag
     5881 gtccgccagc aagcgcatga tctgggcgtc cgagtccgcc accaggaccc ggcctcgcca
     5941 cctcaggggt ctctcgctgg cgcgacgcgc ttcggccacg gacagaaact gcaggatatt
     6001 gtccaggaag ctcttggcgt ccacgggttg ggcaaagtat ccatcgcagc cggcgttcag
     6061 cgccttgcgc tcccctccgt ccaccggctc gggagaaacc gcgataagcg ggatggcctt
     6121 gagggcctcg tcttcccgga tcgagcggac cgcggcgccg gcgtcctcct cgcacaagga
     6181 gaggctcatg aggagacagt cggggcgcac ctcgcgcgcg gcccggattc cggcctccgc
     6241 gctctccgcc tccagcaccc ggaacctgcc gtaggtgagg agcgcccgca ggagactgtg
     6301 gtgctggggg tcgctgccga tcatcaggat cgtctgctgg ttcatgggac ccgttctccg
     6361 gtctcgcagt cgacggcgat catgaggaac gtatccaggc gcggcgcgcc gaaccggacg
     6421 aagccgtccg cgacctggaa cggcagcggc ttctcctccg gaaggaccag cacgcgccgc
     6481 ggacgccgcg cgccgatgtg gagggccacc tgggtaccca ggaccggcac ggcaggaagc
     6541 tccttgggga agttgaccat gctgagcagg aaccgggcgc gatcctcctg ccagaaggtg
     6601 gtgatctcca ccacgcgcgg cgcgtccgtc tgcaccgtga acggttccgc caggcgggag
     6661 agaaggcgga aggtcacccc ttcggctcct tcggccagct cgatgtcccc ggcagcccag
     6721 atggcgcgtc ccttgccgaa ccggtgctcc aggagcgaag gactcttcag cgcgacgccg
     6781 ggagggttgt tgtggataga ggcgaagtgg atcggatcgt cgggattggt gaatgggagc
     6841 gtgagcgtcg ccaggatggt gacatccgct tcggcccgga ccgtgatctg cctcgtgccg
     6901 atccccatgg ggtgctccgc ggtccagtca ccgaagaggc cctctccgtc ccgggtcggt
     6961 gccatgtagg tgaaccgctc tgcggtctcc ccgccataat gcgagccgaa cacatcggca
     7021 aggaggaagt cggggcgccg ccgcccgtcg atcccgatga gggacgtgaa ccggcttgcg
     7081 tacaggttgc ctccggcccg cacgaactcg cggatggccg cggcctcctc atcgtccatc
     7141 aggatcgcgt tgggaacgac gatggtcctc catcgcgaaa gctccgccag gttccgccgg
     7201 gtgatgatcc caaagggaac gtggtgcgcg aacagcaccg aggccactgc cgccacggat
     7261 tccacgtggg gcatccgagt ggatagcgtg ggatcgtcca cgcgcttgcc gttgtcggcg
     7321 agatcgcact tggaggtcag gcccagatag acgaccacgt cctccacgcg ctgcccgccg
     7381 aggtagggct catagacgcg ggtctcctgg aataccctcg ccatgcggtg gtagaccgcc
     7441 gggttcagcg ttccgagcgg gtccacgctg tcgatgaaga cgaaggccgt ggcgtccgcc
     7501 agggccgcgt gcgccttggt tcgaaccagc tccaggggct tcattgccgt gtagttggcc
     7561 aggttggtcg cgatggacgt ttcaaaggcc gccggccgct ggggactcat gttcgagaag
     7621 agctttcgag cgatggaccc ggagggggca tctccgtaga agtcgccctg caggaagtcg
     7681 tttgcgggga ccagccgctc gctcacgccg aacttccagt tgagcgggta ggtggaagcc
     7741 tggtgctcca cgctgacgcc cggctttgcc tgacgcgccg tccgggtcag aagctccgcg
     7801 agctccgcca gccactgctc acgcttcctc tggaacgcca cccaggtcgg atcctcccag
     7861 ttgacccggg tggggagagc tccgccgacc tcgttctgga accgggctcg acaatggggg
     7921 cagtagcaga cgtgcggcca gaaggtcatg tcgaaccgga tgccctcgaa ggagtaccgc
     7981 tcgcatagct cccgggtgaa cgccgcgatg tagtcccggt acggcgagtt gggacagcag
     8041 gttccgtagc ggctttgttc cgcgaccgcg agcccgtcca cgccgatcat cttccatccc
     8101 gggttgtgcc ggtatgccca cgtgtcgaaa atgacgctca ggtacgcgga gaccgcgatc
     8161 ccgcgttcgt ggcagccgtc cacgccttcc gcgaacaggt cacgtccgcc aagccccttg
     8221 tgcggcggaa tgacgttgga ggggtacagc gccaggccca cgtgacccat ggcgtatacc
     8281 accgcggagg ccgcgccgga gcgcgccacc agctcggccc attggcggcc gtcataccgg
     8341 gtgaggaacc gctcgtcgtc gtcggggatg tgcatgtcga ggacggctct gcgccacgct
     8401 ttgcggtacc agggctgctc catgaccccc ccttgagctt atccgggcct gcgggcgacg
     8461 acctccggtt gcgccaggag gccgcacggt accaggctgt acccgtcctt ccagagctct
     8521 cccttggtgg tgaactccgc gggaccggtc caggccggcc acgaggcggc gtggtgcagt
     8581 gggccgtgag agttccttcg gtgcccgatc acctcgatgc ggatctccac ggagcccggc
     8641 gcctctggca ggaggctcgt gatgtcgacc tccggcgggt cccacgcggt gacgcctgcc
     8701 ggccttccgt ccaccagcac ccgagcggcc acgccccgat actccgggac ctccaccacg
     8761 atacgctctt ccggcgatcg ctcgatggcg actctgcggc agtagcagac cgagccggag
     8821 tagaacggca acccctggtg gatccagctt cccagcgcca ggctgcgggg cgcgtcgatc
     8881 atgaacacct cggtgtcctt gacctccacg ccgaactctc ccaggaggta gacgatttcg
     8941 agacccggat gctcctcgtc gtacggacct ctcaacgtga gcgtgttctc gccatggagc
     9001 agaagggcgg gatctaccgg cagcctccga agggacgggt ccacccagaa cccgttgtct
     9061 ccgtcggagc agatctctgc cccgttcaat gagatcgcgt accgcgaagg gtcctcgacg
     9121 cagagggaga gctcaccggt ggggatggcc tccacctcga agcgatagtg cagctctacc
     9181 tccacccgcc gcggcgagcg ggtccgtttt cgcgcccacg gttgtaccat ggcgccaccc
     9241 cgcggcggga cccctaccgc cgcccgcagc gcgccatcca cccgcagtac ctcctcgggt
     9301 ccctgccagg atccgccgtc cacccggtag ctggggcggt ccaggaccag cacgttggct
     9361 tccgatcgaa ggatggtcca tcgaccgtcc tccagcggga tgcggcgatg gtccaccaac
     9421 gggggtcggc gctcccgttt ctgcgcctcc ggcttccttg ggaaaaggaa gagccgcgag
     9481 ccgttggcgg ggaaggaggt ggcgatctgc catgccccgt cctccgcccg cagggaatcc
     9541 gcctggaaga tctcgcccgt ggaagggtcg agctccaggg gctcaccatc acaggccagg
     9601 cgagcgcgga cggtcacgtt ggggaaggcc agcgtgtgct cgctgatgcg gcccgtgtcg
     9661 aagatgtcac ggccacgatc ctcgtccgtg aggctggtat tgcagaggaa caggtagtag
     9721 gcgtccttgt cctcccgaag ctggtacagg atggacgcca gctcgcggtc ttcggcgtcc
     9781 gtgacggaga cccgtcgggc agccttgtcc accgcggcgg cgagctgcgg gcccgcgggc
     9841 ggcgcggcca cgcactgggc cgccaggtcc gccggagcat ccgacgccgc ggcgtccacg
     9901 taccgcggag gctctccggt gaagaccacc aggccgccgg cggcctggaa ctccgccagc
     9961 agcatcagtg tcgaggaccg gatggtccga agcgggggaa ccacgaccgc ggcatagcag
    10021 gcacggccga cgaccaggag cggcgtgccc ttgcgccgag acaccgcggc gagccgggag
    10081 agcatctcct catcgccata atcgaagtcg aggtgcgccc ccaggagcgt gtcctccagg
    10141 tccaccagga ctcggtcgta atcctggacc tccttctccg ccagccatcc gcggcggatc
    10201 atggtccacg tgctctccac cggatggatc accaggaggt cccgcacttc ctctccccgg
    10261 ctcaggaggg tggccacccg ggagaaatag tcctccacga cgggatacag ctcccaccac
    10321 ggagactgcg cggaaatggc ggcggggtag tcgcgcttgg cttccccccg catggtgtac
    10381 caggccaggt gctggcagcg caggttgatg cccagggcga actgccagtc gaagatcgcc
    10441 ttgtgcccga cgaacgggaa atcccagccc gtggcgccgt agctctcgct gatccgccac
    10501 cgcttcccga gctggtgagc cacggagctg acctgcttcg ccgtggcata ggcgcgccag
    10561 cgctccgtca gcaggtcgat gcccggggcc tgcatgggct cgtagaaccg catggcggat
    10621 cccaccacgc acgcctggct ggagagacgg tcctccatca gcacgtggcc ggtgtgcatc
    10681 agcccgtgct cctcacacca ggctccgatc tgtcggctga aggaatccac gaagagcgcg
    10741 gtgaggcagt cgtggaaatc gtggcgcgcc ggagtgactt gctgcccgtc gacgtccagg
    10801 tacagctcca ccaggtgcgg gatgaggtcg tagccgtagc gcttcttgaa cgccgccggc
    10861 aacccctccg tccacggcag gtccagcggc gagccggtgt tgttgtctcg atcgaacttc
    10921 tgcccatggt tgggctcgtc ggtgaagata ccggggagcg tctttccgaa gtggcggccc
    10981 acgtggcggc ggtattcctc gtgggtcacc gcgatgaacc ggcgcaccgc agccgggttc
    11041 agggtgtcca ggtaggtggc atcgttgtac cagctcgaga cgggatctac caggacccgg
    11101 aacgacagga tgcgctctcc atgggaaagg gaggaaggcc gtctgccgcg cggtatgggt
    11161 tggactcccg aggcggtggt gcccttcacc gtcgagcgga acgcggctac cacctccgcg
    11221 gtccatcgaa gctgcgccgg ggccttgagc tcctccatca gcagcgagcg ctttcggtac
    11281 cggggattgc gggttaccag gccgccggcg gctcccgaag gccaacggtc gtcatcgtac
    11341 agccaggcga gcatgttctg cttctgcgcc tcgtcgatgc agacctctat cgaacggaac
    11401 cactcgtccc gcaggtaggg agtggacaga ccgatgcggg aatgcatgaa gaagccgccc
    11461 atccccattc gcttcatcaa gcggatctgg cgtcgcagct cctgaggctc cagggccccg
    11521 ttccacgccc agaagggctg accccggaac caggggccgg gcttcgcgaa gtcattctcc
    11581 agactacgtt ccaactctca cgctcctctt caggatgacg tccctggcgc gacgctcgtg
    11641 ttcattcggc caacacctgt tcctcacgct cgcggaagta gcgggccgtc tgctggacga
    11701 gctcttccag ggccgcgggg tcgggcttgc cgttc
//
LOCUS       DASRZS010000180         4759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4332385, whole genome shotgun sequence.
ACCESSION   DASRZS010000180 DASRZS010000000
VERSION     DASRZS010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4332385"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /locus_tag="VFH83_04460"
     CDS             complement(<1..2004)
                     /locus_tag="VFH83_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6485647.1"
                     /translation="MRALVPLLYALKRIASSYALALCILAGFTASVALSICIPLYSTA
                     VSTRMLSEELAKQPLPPYAFLFTFNGPLKKDHDRTEFTRLDGSFDREVPQALGLPALS
                     RVRHVSVDTMQLLPIQGSGYSDPSSPLDWINLGSITGLETHLELIEGAAPHPTSAGQP
                     VEVIVTQPEAEKLGLEVGDRFLLAIDKEEAGRHLQLAVPVRVSGVWRPKDQDRSFWFY
                     QPSAYDQMVLTDEASFWSATRGATDGVPFDVTWYLVFDGSRFRVDQTQRLLSGIARVK
                     AELASFESRPSLIYSPEESLHKYSAAGSQLTILLFAFALPVFALIFYLIFLLAHIAVE
                     RRKNEIALLRSRGTSRRRVVWTQLLQTLLLVAAAIPLGAALGMVFAQAMERTRTFLQF
                     EIRTVPPVRISGSAVAVAAVAAACTLVGAILPSVRASRDTIVSYKRERARSGRRPAWE
                     RFYLDVALFVVTAYGYYTLRSRGTLMAFLSGGAVDDPFKNPFLFLVPTLFLVSVSLIL
                     LRLFPVLMEGIAGALRRLRGAPLVMAARAIARSPGSNRGLLLLVMLTVGLAGYTASMA
                     LTLDRHTTTDVYYSMGADLVLNENGAYAAVDATGSGDSTASTGSTTASGQTSGAPASG
                     ASDAERWEYPPISDYTELPGVHGGTRVGTYKAEVRWTGKLETGK"
     gene            complement(2001..3320)
                     /locus_tag="VFH83_04465"
     CDS             complement(2001..3320)
                     /locus_tag="VFH83_04465"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET6485648.1"
                     /translation="MNRLIVPALAVVILASCYRAPVAKDDQLLAAAQASPQVEEATAV
                     VARGDVTLQTRFLGTVEPVDEKMLFFRVDGRIRVLRVKEGDRVRAGQVLAELEMSDLE
                     DQIAQATVALEKTRARVDASQQRSRDLQEAQIKEQIAELTLQQAQTEDPSAEVAIAKA
                     ALDKADLAVADAKLANQIQQKRDSAQAVQRAQLDEQVAAQSYQLALQAQQSHQYQLKI
                     LEQQVELARVAVDRLKDPADPAAAADVSIAELTVNQLQDRAAMYRIVAPTDGQVLSLT
                     KAAGDEASAFQTVAIVADPSRLEVSASLMADQVIRLHVGQVVSLQLGEAGGQKVPGRI
                     RRIPFASQGSSSPAEADQTTRISFAAPPVEDLKPGDPVRITVVLDQRKDVLYIPKSAA
                     RSFRDRTFAVVVDGENRRRVLLQIGLEGDDDVEVMSGLQEGQRVVLQ"
     gene            3393..>4759
                     /locus_tag="VFH83_04470"
     CDS             3393..>4759
                     /locus_tag="VFH83_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485649.1"
                     /translation="MSEIKAYLVPFSHMDLFWLGAQEECLSRGNRIISEAMDLAEQYP
                     EFRFLIEDLVFIDHFLRCHPERKAALKGLLDRGRIEIGPKWAGIDQTPQLGEDLVRNS
                     LYALQYLERELGYASRTMHTGDLPGWTPQYPQILTRLGVPYAVYTRTGPTDVSLFQWK
                     GLDGTRLLVWYSLNGYTWAWHDGGLEVSAEKAKANGLEETMTKVLAQGVSPIFVHWGV
                     DLILPGRKLPENLREWNRTSPHTMAFATPTQYFDAAPKTGLRELTGEVPSAWPYSEPQ
                     YPESMVYTYPAVSGLLDAESYSALALVHGHTTAYPGEKIREGWLKTLEAMDHNNNGNG
                     YDQTRERKREYFQSAARTAVRIREDMLRRMAEKVESPFGTSGYPVVVFNPTGWRRSDI
                     VRTHATFHGDISAFEITPYRRISLVDHEGNPVPFQTSDIREGVARELWLSFVAKDVPA
                     NGYRT"
BASE COUNT          799 a         1590 c         1627 g          743 t
ORIGIN      
        1 cttccccgtc tccagcttcc cggtccagcg gacctccgcc ttgtaggtcc ccacccgggt
       61 tccgccatgg acgcccggca gctccgtgta gtcggagatc ggagggtact cccagcgctc
      121 cgcatcggac gcgccagagg cgggggcgcc ggaggtctga ccgcttgcgg tcgtgctgcc
      181 ggtggaggcc gtggaatcgc cggaccccgt ggcgtctacc gccgcgtagg ctccgttctc
      241 gttcagcacg aggtcggcgc ccatggaata gtacacgtcc gtcgtggtgt gccggtccaa
      301 cgtcagcgcc atggaggcgg tgtagccggc aagccccacc gtcagcatga ccaggagcag
      361 gaggccccgg ttggatcccg gggatcgcgc gatggcccgc gccgccatga ccaggggagc
      421 gccccgaagc cgccgcaatg cgcccgcaat cccctccatg agcaccggga agagccgaag
      481 gagaatcagg gataccgaga cgagaaacag cgtggggacg aggaacagga aggggttctt
      541 gaacgggtcg tccaccgccc cgccgctcag gaaggccatg agcgtcccgc ggcttcgaag
      601 cgtgtagtat ccgtacgcgg tcacgacgaa gagcgctaca tccaggtaga accgctccca
      661 ggcgggtctt cgaccggacc gcgctcgctc ccgcttgtag ctcacgatgg tgtcccggga
      721 ggcgcggacc gagggcagga tggcgcctac cagcgtgcac gcggcggcta cggcggcaac
      781 ggctactgcg gatccggaga tacggaccgg cggcaccgtc cggatctcga actggagaaa
      841 cgtccgggta cgctccatcg cctgggcaaa gaccatcccc aacgcggcgc ctagcggaat
      901 ggccgccgca accaggagga gggtctggag gagctgggtc cagaccacgc gccgccggct
      961 ggttccgcgg ctgcgcagga gcgcgatctc gttcttccgt ctctcgacgg cgatatgcgc
     1021 caggaggaag atgaggtaga agatgagcgc gaacaccgga agggcaaacg cgaagaggag
     1081 aatcgtgagc tggctgccgg cggcgctgta cttgtggagg gactcctccg gagagtagat
     1141 gaggctggga cggctctcga aggatgccag ctccgccttc acgcgcgcga tcccgctcag
     1201 gaggcgctgg gtctggtcca cgcggaagcg gctcccgtcg aagaccaggt accaggtcac
     1261 gtcgaacggg acgccgtcgg tggcgccgcg cgtggccgac cagaagctcg cctcgtcggt
     1321 caggaccatc tggtcgtagg cgctcggctg gtagaaccag aaggatcggt cttggtcctt
     1381 gggcctccaa accccggaga cccgcacggg aaccgcgagc tggaggtgcc tccccgcctc
     1441 ttccttgtcg atggccagca ggaaccgatc ccccacctcg agccccagct tctccgcctc
     1501 gggctgcgtc acgatgacct ccaccggctg cccggcggaa gtgggatgcg gagccgcgcc
     1561 ctcgatcagc tccaggtgcg tctcgagccc ggtgatcgac cccaggttga tccagtccag
     1621 aggggaggac gggtcggagt atcccgagcc ctggatgggc aggagctgca tcgtgtccac
     1681 ggaaacgtgc cgcacccgtg agagcgccgg aagccccagg gcctgcggca cttcccggtc
     1741 gaaggagccg tcgaggcgcg tgaactccgt ccgatcgtgg tccttcttaa gcggcccgtt
     1801 gaacgtgaag aggaaggcat acggagggag cggctgcttt gccagctcct cggaaagcat
     1861 ccgcgtgctg acggcggtgg agtagagagg gatgcagatg gagagggcca cgctggcggt
     1921 gaatccggcc aggatgcaga gggcaagggc gtagctgctg gcaatccgct tcagcgcgta
     1981 gagcagcggg accagcgccc tcactgcagg accacccgct gtccttcctg gaggccggac
     2041 atcacctcca cgtcgtcgtc cccttccaat ccgatctgca agaggacgcg gcggcggttc
     2101 tccccgtcca ccacgacggc gaacgtccga tcgcggaagc ttcgggccgc gctcttgggg
     2161 atgtagagca cgtccttgcg ctggtccagg accacggtga tccggacggg atcccccggc
     2221 ttcaggtctt ccaccggcgg agcggcgaag ctgatccggg tcgtctggtc cgcctccgcc
     2281 ggggaggacg atccctggga ggcaaacgga atccggcgga ttctccccgg caccttctgg
     2341 ccccccgcct ctccgagctg gagcgagacg acctggccca cgtgcagccg gatcacctgg
     2401 tccgccatga ggctcgcgct cacttccagg cggctcgggt cggccacgat ggccacggtc
     2461 tgaaaggcgg aggcctcatc ccccgcggcc ttggtcaggg acaggacctg gccgtcggtc
     2521 ggcgccacga tgcggtacat cgccgcgcgg tcctggagct ggttcacggt gagctctgcg
     2581 atggaaacgt ccgccgcggc ggccggatcc gcggggtcct tgagccggtc caccgccacc
     2641 cgggccagct ccacctgctg ctccaggatc ttgagctggt actggtggga ctgctgggcc
     2701 tgaagagcga gctggtagct ctgggccgcc acctgctcgt cgagctgcgc gcgctggacg
     2761 gcctgggccg aatcccgctt ctgctggatc tggttggcga gcttcgcgtc cgccacggcg
     2821 aggtccgcct tgtccagggc ggccttggcg atggcgacct ccgcggaggg atcctccgtc
     2881 tgcgcctgct gaagggtgag ctcggcgatc tgctccttga tttgggcttc ctggaggtcg
     2941 cggctcctct gctgggaggc gtcgacgcgc gcgcgcgtct tctccagggc cacggtggcc
     3001 tgggcgatct ggtcttccag gtccgacatc tccagctccg ccaggacctg gcccgcgcgg
     3061 acccgatcac cttccttcac ccgcaggacg cggatccttc cgtccacgcg gaagaagagc
     3121 atcttctcgt ccaccggctc cacggtcccc aggaaccgcg tctgcagggt cacgtccccg
     3181 cgggccacca ccgcggttgc ctcctccacc tggggcgatg cctgggcggc ggcgaggagc
     3241 tgatcatcct tggccacggg cgcgcggtag cagctcgcca ggatgaccac cgcgagcgcc
     3301 ggcacaatga gacgattcat gctgaatcct cgccgatggt ataatacgcg agcccgcgac
     3361 ctgcaaggcg gctcgatgga cggaggcgaa gcgtgagcga gatcaaggcc tacctggttc
     3421 ccttctctca catggacctc ttctggctgg gcgctcagga ggagtgcctc tcccggggca
     3481 accggatcat ctcggaggcc atggacctgg cggagcagta ccccgagttc cggttcctca
     3541 tcgaggatct cgtgttcatc gatcacttcc tgcggtgcca ccccgagcgc aaggccgctc
     3601 tgaaggggct cctggatcga ggccggatcg agatcggacc caagtgggcc ggcatcgacc
     3661 agacgccgca gctcggagag gacctggtcc gcaacagcct gtacgcgctg cagtacctgg
     3721 agcgcgagct tgggtacgcg tccaggacca tgcacacggg cgaccttccc ggctggacgc
     3781 cgcagtatcc gcagattctc acaaggctcg gcgtgcccta cgcggtgtac acccgcaccg
     3841 ggcccacgga tgtctccctg ttccagtgga agggtctcga cgggacgcgg ctcctggtct
     3901 ggtattcgct gaatggctac acctgggcgt ggcacgacgg cgggctcgaa gtcagcgcgg
     3961 agaaggcgaa ggccaacggc ctggaagaga ccatgaccaa ggtcctggcc cagggagtca
     4021 gccccatctt cgtccactgg ggcgtggacc tgatcctgcc gggccgcaag ctcccggaga
     4081 acctgcggga gtggaaccgg acctctccgc atacgatggc gttcgcgaca cccacgcagt
     4141 atttcgacgc ggcacccaag accggcctgc gcgagctgac cggcgaggtg ccctccgcct
     4201 ggccgtacag cgagccgcag tatccggagt ccatggtcta cacgtacccc gcggtctcgg
     4261 gcctcctgga cgcggagagc tactcggcgc tggccctggt ccatgggcac acgacggcgt
     4321 atccggggga gaagatccgc gaggggtggc tcaagacgct ggaggccatg gaccacaaca
     4381 acaacgggaa cggctacgac cagacccggg agcgaaagcg cgagtacttc cagagcgcgg
     4441 cgcgcaccgc cgtccgaatc cgtgaggaca tgctgcgccg catggcggag aaggtggagt
     4501 ctccgttcgg gacctccggg tatcccgtgg tggtgttcaa ccccacgggg tggcggcgct
     4561 cggacatcgt ccggacccac gcgaccttcc atggagacat cagcgcgttc gagatcacgc
     4621 cgtatcggcg gatatccctg gtggaccacg agggcaaccc ggttcccttc cagacctccg
     4681 acatccgtga aggcgtggcc agagagctct ggctgagctt cgtggcgaag gatgtccccg
     4741 cgaacggcta ccgcacgta
//
LOCUS       DASRZS010000181         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1252816, whole genome shotgun sequence.
ACCESSION   DASRZS010000181 DASRZS010000000
VERSION     DASRZS010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252816"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VFH83_04475"
     CDS             <1..716
                     /locus_tag="VFH83_04475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6485650.1"
                     /translation="NRMGPDPNAAGLSRKHLFAAIDASLRRLGMDYVDLYQIHRSDPQ
                     TPIDETLDALDDLVRSGKVRYIGASSMPAWLFMKALALSERHGWARFVSMQNHYNLVY
                     REEEREMIPLCADQGIGVIPWSPLARGFLAGNRSPEKSGPTTRSKSDEFAHHLYYQPD
                     DFAVVDRLKTVAAQVGKSMPQVALAWMLSKPFLTAPIVGASKMAHLEQAVEAVSVKLS
                     AEHVKSLEEAYRPHPVMGH"
     gene            complement(775..1818)
                     /gene="holA"
                     /locus_tag="VFH83_04480"
     CDS             complement(775..1818)
                     /gene="holA"
                     /locus_tag="VFH83_04480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET6485651.1"
                     /translation="MSPPSREAAGRPGSGRPGAVQLFLGPEEGQKAEHIEKLRQQIAS
                     RMKGDPEVSRFYASETRMVQILLVARNLSLFSPHRLLIVSNIEEVRRAEDVQALVEYI
                     QSPAPDATVILLGSGYNGDVDRRILSAIPKESQRIFWELFENQKQGWIMAFFRQRSIV
                     IDPAAVEYILDMVENNTRDLRTECERLAQFFGTGSTIGLEAVEQYLYHSKEENVFTLF
                     DRLCERDFPASIEVLDKILLAREADPTQLSAGLLAQFRKLGSLKRMIEESFEPSEAFT
                     RLRILSKRNQRTYQEGSRHFSGADLQSIVLLLATFDERLRLTKAEAHGLLLHLVIYYI
                     TRRAGQGAWRMER"
     gene            complement(1815..>2283)
                     /gene="lexA"
                     /locus_tag="VFH83_04485"
     CDS             complement(1815..>2283)
                     /gene="lexA"
                     /locus_tag="VFH83_04485"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET6485652.1"
                     /translation="KALEKKGFLKSHENRSRALEVVRGAETDEEAVAPVPLLGNVAAG
                     KPLFAEENLQGYVYVPAAALGKATHFALRVRGDSMKDAGIMDGDVVVVSQQATADNGD
                     IVVAMVDEAVTLKRFFAEKNRVRLKAENPKYPPIFTQNVRILGKLSFLMRTYA"
BASE COUNT          378 a          755 c          743 g          407 t
ORIGIN      
        1 tcaaccggat gggaccagat cccaacgctg cgggtctgtc tcggaaacac ctcttcgcgg
       61 cgatcgacgc gtcgctgcgc aggctgggca tggactacgt cgatctctac cagatccacc
      121 gttcggaccc tcagacgccg atcgacgaga ccctggatgc gctcgacgac ctggtgcgct
      181 ccgggaaggt ccgctacatc ggagcctcca gcatgccggc ctggctgttc atgaaggccc
      241 tcgccctctc ggagcgtcac ggctgggccc ggtttgtcag catgcagaac cactacaacc
      301 tggtgtatcg ggaggaggag cgtgagatga tccccctctg cgcggaccag ggcatcggcg
      361 tcatcccgtg gagcccgctt gcccgcggct tcctggccgg caaccgctcg ccggagaaga
      421 gcgggccgac cacccgctcc aagtcggacg agtttgccca ccacctctac taccagccgg
      481 acgacttcgc ggtcgtggat cggctgaaga ccgtggccgc ccaggtgggc aagagcatgc
      541 cccaggtcgc cctggcgtgg atgctgtcca agccgttcct tacggcaccc atcgtcggag
      601 cgagcaagat ggcgcacctg gagcaggccg tggaagccgt ctcggtgaag ctcagcgcgg
      661 agcacgtaaa gagcctggag gaagcctacc gccctcatcc cgtgatgggg cactgatcgg
      721 gagggcgatc aggagaaggc gtggtgccgc gggggccagc gccggcgagc gaccctaccg
      781 ctccattcgc caggcgcctt gccccgcccg gcgggtgatg taatagatca cgaggtgcag
      841 gagcagtccg tgcgcctccg ctttggtgag ccgcagccgc tcgtcgaacg tcgccaacag
      901 gagcacgatg gactgcaggt cggctccgga gaagtgtcgg ctgccctcct ggtaggttcg
      961 ctgattgcgc ttgctcagga tgcgcagccg ggtgaacgcc tccgagggct cgaagctctc
     1021 ctcgatcatg cgcttcaggg agcccagctt tcggaactgg gcgaggagcc cggcggagag
     1081 ctgggtggga tccgcctccc gcgccagcag gatcttgtcc agcacctcga tggaggccgg
     1141 gaagtcccgc tcgcagagac ggtcgaagag cgtgaatacg ttctcttcct tcgaatggta
     1201 aaggtactgc tccacggcct ccaggccgat cgtcgagccc gtgccgaaga actgggccag
     1261 ccgctcacac tccgtacgca ggtcccgggt gttgttctcc accatgtcca ggatgtactc
     1321 caccgccgcc ggatcgatga cgatcgagcg ctggcggaag aacgccatga tccatccctg
     1381 cttctggttc tcgaacagct cccagaagat gcgctgggat tccttgggga tggccgacag
     1441 gatgcgtcgg tccacgtctc cgttgtagcc gctgcccagg aggatcacgg tggcgtccgg
     1501 cgccgggctc tggatgtatt ccaccagcgc ctggacgtcc tctgcccgcc tcacctcttc
     1561 gatattggag acgatcagaa gccgatgggg ggagaacagg ctcaggttac gggctaccag
     1621 gaggatctgg accatgcggg tttccgatgc gtagaaccgg gagacctccg ggtctccctt
     1681 catccgggag gcgatctgct ggcgcagctt ctcgatgtgc tcggccttct ggccttcctc
     1741 cgggccgaga aagagctgga ccgcaccggg acggcccgag ccagggcggc cggccgcctc
     1801 acgcgacgga gggctcacgc gtaggttctc atcaggaagg atagcttgcc caggatccgg
     1861 acgttctgcg tgaagatagg cgggtacttg gggttctcgg ccttgagccg cacgcggttc
     1921 ttttccgcaa agaaccgctt gagggtcacc gcctcgtcca ccatggccac gacgatgtcg
     1981 ccgttgtccg ccgttgcctg ctgggagacc accaccacgt ccccgtccat gatgccggcg
     2041 tctttcatgc tgtctccgcg cacccgaagc gcgaagtgcg tggcctttcc cagggcggcg
     2101 gcgggcacgt atacgtaccc ctggaggttc tcctccgcga agagcggctt gccggcggcg
     2161 acgttgccca ggagcggcac gggcgccacg gcctcctcat cggtctccgc ccctcggacg
     2221 acttccagcg ctcgggatcg attctcgtgg ctcttgagga accccttctt ctccagcgcc
     2281 ttg
//
LOCUS       DASRZS010000182         2180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5101075, whole genome shotgun sequence.
ACCESSION   DASRZS010000182 DASRZS010000000
VERSION     DASRZS010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5101075"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFH83_04490"
     CDS             complement(<1..896)
                     /locus_tag="VFH83_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485653.1"
                     /translation="MADRDPIKVYDARWEVDEFDRNEVARLVEATLMYGRLLGVDAVT
                     ICRDARVGCAALAELAMDIALGSGFEVLLCPDPVSTPQSYFVTFQASAAHPRTMGLTF
                     TASHNPAAYVGLKVVVPPVQAIGLDCGPLGGLRKVRELYHGGEPLPGGGRGRLHMQSL
                     AGDYVEFSMDAAGLAPGSLAGLTVVFDFFNGSAGPEVMDALQRSGASVVPLHLVPDGT
                     FPAGSPNPTSAGKMAAAVARAREAGRAVVVGTDGDGDRLVFGDGTGVLSAGIAAIPIL
                     SLTVPEKRGEREKVLYDPKVSPL"
     gene            complement(905..2110)
                     /locus_tag="VFH83_04495"
     CDS             complement(905..2110)
                     /locus_tag="VFH83_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET6485654.1"
                     /translation="MRKSARAVQGIRDSNQKKILALLIEKRQLSKQEIARETSLSIPT
                     VTTNVSRLLELGLAAEAGVSASTGGRKPMIIRFLPDSRYAFGVDFSSNHLTSSNLVRV
                     VLVNLDAQIRSQSSFPYGDFSSVGQIITHVKKLTAKMLAERRIPRHLVMGIGFSLPGT
                     VNERKLLLEQAPNLPPDLGMNALDFRRFQALFPWPILVENEANASAYAELVLGGARRK
                     RSLVFLSINRGIGAGIVVGGHVFKGSNKRAGTVGHITVESKGVRCTCGRRDCWEIYAA
                     SGALIRNYRKVSGKKLRDTRQFHRLLEDADPAAEKIWDEYLEYMAIGINNIILSLDPH
                     SIIIGGEISRFGDRLLAPLRAKVFAQNTFYRDDDLEIAFSRLGGDASILGVALLPFHG
                     LVHQGAKVI"
BASE COUNT          340 a          737 c          732 g          371 t
ORIGIN      
        1 agggggctga ccttcggatc gtagagcacc ttctcccttt cgccgcgctt ctccggcacg
       61 gtgagcgaca ggatggggat tgccgcgatc ccggcgctga gcacgccggt gccgtcgccg
      121 aagaccaggc gatccccgtc gccgtcggtg cccaccacga cggcacgccc ggcttcccgt
      181 gcccgcgcga cggcggcggc catcttgccc gcgctggtgg gattgggaga gccggcaggg
      241 aaggtcccgt cggggaccag gtgcagaggc acgacagagg cgcctgaccg ctgcagggcg
      301 tccatcacct ccggtcccgc ggaaccgttg aaaaagtcga agacgaccgt gaggcccgcc
      361 aggcttcccg gggcgagacc cgccgcgtcc atggagaact ccacgtagtc acccgccagg
      421 ctctgcatgt gcagccgtcc gcggcccccg cccggcaggg gctccccgcc gtggtagagc
      481 tcgcggacct tccgcagtcc tcccaggggt ccgcagtcca gcccgatggc ctggaccggg
      541 ggaaccacca ccttcagccc gacgtacgcg gccgggttgt gcgacgcggt gaacgtgagg
      601 cccatggtgc gcgggtgggc ggccgatgcc tggaaggtca cgaagtagct ctgcggggtg
      661 ctcaccgggt ccgggcagag gagcacctcg aagcccgatc ccagggcgat gtccattgcg
      721 agctccgcga gggcagcgca gcccacccgc gcgtcgcggc agatggtcac ggcatccacg
      781 ccgaggagcc tgccgtacat cagggttgct tccaccagcc gggcgacctc gttgcggtcg
      841 aactcgtcga cctcccagcg cgcgtcgtag actttgatcg ggtcccggtc cgccatgccc
      901 cggctcagat gaccttcgcg ccctggtgga ccaggccgtg gaatggcagc agggcgacgc
      961 cgaggatgga tgcgtctcca cccagccggg agaaagcgat ctccaggtcg tcgtcccggt
     1021 agaacgtgtt ctgtgcgaac accttggcgc gcaggggcgc gagcagccga tcgccgaagc
     1081 ggctgatctc gcccccgatg atgatcgagt gcgggtcgag gctgaggatg atgttgttga
     1141 tcccgatggc catgtactcg aggtactcgt cccagatctt ctccgccgcg ggatccgcgt
     1201 cctccagcag ccggtggaat tgccgggtgt ccctgagctt cttccccgag accttgcggt
     1261 agttgcggat cagggcgccg gaggcggcgt agatctccca gcagtccctg cgcccgcagg
     1321 tgcagcggac ccccttggac tccacggtga tgtgccccac ggtccccgcg cgcttgttgc
     1381 tccccttgaa cacgtgtccg ccgaccacga ttcccgcccc gattcccctg ttgatggaga
     1441 ggaacaccag gctgcgcttc ctgcgggccc ctcccaggac cagctccgcg taggcggacg
     1501 cgttcgcctc gttctcgacg aggatcggcc aggggaacag ggcctggaac ctgcggaagt
     1561 ccagggcatt catgccgaga tccggtggaa ggttgggcgc ctgctccagg aggagcttcc
     1621 gctcgttgac cgttcccggc agggagaagc cgatgcccat cacgaggtgc cgggggatcc
     1681 tccgttccgc gagcatcttc gccgtcagct tcttcacgtg cgtgatgatc tggcccaccg
     1741 aggagaagtc gccgtacggg aagctggact gcgacctgat ctgggcgtcg agattgacga
     1801 ggacgaccct gaccaggttg ctcgacgtga ggtggttgga ggagaagtcc acgccgaacg
     1861 cgtagcggga gtccgggagg aagcggatga tcatgggctt cctcccgccc gtggaggccg
     1921 agacgcccgc ttccgcggcc aggccgagct caagcagccg ggacacgttc gtggtcaccg
     1981 tggggatgct gagggaggtc tcccgcgcga tctcctgctt gctgagctgc ctcttctcga
     2041 tcagcagcgc caggatcttc ttctggttcg agtccctgat tccctgcacc gcgcgcgcgc
     2101 tcttcctcat ctgtcctccc acgggggact ccctgcttgt accagcagga cctcactttg
     2161 tcaatcgtct ttaaaaagta
//
LOCUS       DASRZS010000183         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1634387, whole genome shotgun sequence.
ACCESSION   DASRZS010000183 DASRZS010000000
VERSION     DASRZS010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634387"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VFH83_04500"
     CDS             <1..510
                     /locus_tag="VFH83_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET6485655.1"
                     /translation="GGNGLSFAAHAVRLGAAVWQVGSRRCQTAALGLGVRAFLDYQKE
                     DSAETLGAACPDGFDLIVDAVGKAGAMDRFLPLLAAGGTLAIYGLDDFQRISVSPRLA
                     RGTFTVYGGGYDEAETHQRVSEMALQGLLSSGAWYNAARPYPLSRINDAFADLWNRKA
                     VKALIQLSA"
     gene            752..1954
                     /locus_tag="VFH83_04505"
     CDS             752..1954
                     /locus_tag="VFH83_04505"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET6485656.1"
                     /translation="MLNLRHVKRHAGTVPQPFVDQVDPATYSRSVAYTVSRGSFGLLA
                     SAVNAAVTLAVVLTGALGFLDRLVSAVPIHRYLQGELFIAVLAVLLWIIGLPFALIST
                     FSIEARYGFNRTTPRLYLVDTLKSLGISVVLGTIVLLGLFWFMDSTGRFWWIWAFGAM
                     TVLELILTILGPLVIAPLFNKFTPIAEGELKDHILALARKLGFRTSGIFVVDASRRSR
                     HSNAYFTGIGRVKRIVLFDTLVNTSTEEEILSVLAHEIGHEKKNHVRKGLAISLVLSL
                     AGFWILSLLLPWAPFYQAFGLQHPSYPGILVLLALCSGPFTFVLQPLFSMRSRHQEYE
                     ADRFAVQGVGSAAGLKSALLRLSKDNLSNLTPHPLYSFIHYSHPTPAERIAALDRAEA
                     ALKLASPR"
     gene            complement(1873..2193)
                     /locus_tag="VFH83_04510"
     CDS             complement(1873..2193)
                     /locus_tag="VFH83_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485657.1"
                     /translation="MTKRAGSSVANRTGDPLPLDNSYLIQVARNTLETIKSYVDKIGI
                     HIADQRPVHDNEVILGEIGSAPPTPVSARPMPPCRPQRGDASLSAASARSSAAILSAG
                     VGCE"
     gene            2293..2460
                     /locus_tag="VFH83_04515"
     CDS             2293..2460
                     /locus_tag="VFH83_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485658.1"
                     /translation="MCDLLRFRHRLGRDLASEALRDYLKRPRRRINTMLEYAAKPHVL
                     GRLRASLEALL"
     gene            2457..>2715
                     /locus_tag="VFH83_04520"
     CDS             2457..>2715
                     /locus_tag="VFH83_04520"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HET6485659.1"
                     /translation="MKTSVRARLEAKARERGTTFHYASLLYMQEGFLARLAASPHADK
                     LILKGGFLLFSTRAPMGRTTRDIDFLGEGLPNEETSLVRIVA"
BASE COUNT          403 a          936 c          862 g          514 t
ORIGIN      
        1 ggcggcaacg gcctttcctt cgccgcgcac gcggtccgac tcggcgcggc cgtctggcag
       61 gtcggttcgc ggcgctgcca gaccgcggcg ctcgggctgg gggtgcgggc attcctggac
      121 tatcaaaagg aagacagcgc ggagacgttg ggggcggctt gcccggacgg cttcgacctg
      181 atcgtcgatg ccgttgggaa ggcgggtgcg atggatcggt tccttccgct cctggctgcc
      241 ggaggcacgc tcgccatcta cggactggat gacttccagc gaatctccgt ctcgccgaga
      301 ctcgcccgag ggaccttcac cgtctacggg ggcggatacg acgaggcgga gacgcaccag
      361 cgggtctccg agatggctct tcagggactg ctctcctccg gggcctggta caacgcggcc
      421 cggccctatc cgctttcccg gatcaacgac gcgttcgccg atctgtggaa tcggaaagcg
      481 gtgaaggcgc tcattcagct ttcggcttga gccgggcctc gcagactgcg ggccctccca
      541 ggcggctgcc tccgggcggc ggccctccct ggcggcggtt gccgacctcc gggcggcggt
      601 ccctccctgg cggcggttgc cgaccaccgg gcgggcttgt agcttaggat gagattggga
      661 cgaaaggaga acgccgctga gccccaccac gatccttgtc ctgttcctga tcctcttcgc
      721 gctcgactac ctctgggaca cgagcctcag catcctcaac ctccgccacg tcaagcggca
      781 cgcgggcacc gtcccacagc cgttcgtcga ccaggtggat ccggccacct attcccgctc
      841 ggtggcctac acggtgagtc gcggctcctt cgggctcctg gccagcgccg tgaacgcggc
      901 ggtgactctg gcggtggtgc tcaccggcgc ccttgggttc ctggaccgcc tcgtctccgc
      961 ggtacccatc caccgctacc tccagggcga gctcttcatc gccgtcctcg cggtgctcct
     1021 ctggatcatc ggtcttccgt tcgccctgat ctcgacgttc tcgatcgagg cccgttacgg
     1081 cttcaaccgg accaccccac ggctctacct ggtcgatacc ctcaagagcc tgggtatctc
     1141 cgtggtgctc ggcaccatcg tgctcctcgg acttttctgg ttcatggaca gcacgggccg
     1201 gttctggtgg atctgggcct tcggcgcgat gacggtcctg gagctgatcc tgacgatcct
     1261 gggcccgctg gtgatcgcgc ctttgttcaa caagttcacg ccgatcgcgg agggcgagct
     1321 caaggaccac atcctggccc tggcgcgcaa gctgggcttc cgcacgagcg gcatcttcgt
     1381 ggtggatgcc agcaggcgat ctcgccactc caacgcgtac ttcaccggca taggccgggt
     1441 aaagcggatc gtcctcttcg acacgctggt gaacacgagc acggaggagg agatcctctc
     1501 ggtcctggcc cacgagatcg gccacgagaa gaagaaccac gtgcggaagg gactcgccat
     1561 ctccctggtc ctgtccctgg cgggattctg gattctaagc ctgctccttc cctgggctcc
     1621 cttctaccag gcattcggcc tccagcatcc cagctacccc gggatcctgg tcctcctggc
     1681 gctctgctcg ggacccttta cgttcgtcct gcagcccctc ttttccatgc gctcgcgcca
     1741 ccaggagtac gaggccgacc gatttgccgt gcagggcgtg ggaagcgcgg cggggctgaa
     1801 gtccgcgctc ctgcggctct ccaaggacaa cctgagcaac ctgacgccgc acccgctgta
     1861 cagcttcatc cactactcgc accccacgcc ggcggagagg atcgcggcgc tggaccgcgc
     1921 ggaggccgcg ctcaaactcg cgtcgccgcg ctgaggccgg cacgggggca tgggtctcgc
     1981 tgaaaccggc gtgggcggcg cggagccgat ctctcccagg atcacttcgt tgtcatgaac
     2041 gggccgttgg tcggcgatat ggatcccgat cttgtccacg tagctcttta tggtctccag
     2101 cgtgttgcgc gcgacctgga tcaggtagct gttgtccaga ggtagtgggt ctccggttcg
     2161 gttggcgacg gacgatccgg cccgcttggt catcacggat ccttctgagg ttcgcacgtt
     2221 cctgcgccat cgccccggtc agaacgccgt tcgtaggacg gtacgctccg gctgtacgac
     2281 gccgaacgga ccgtgtgcga cctcttacga ttccggcatc gcctgggcag ggacctggcc
     2341 tccgaagccc ttcgggacta cctcaagcga ccgcggcgca ggatcaacac gatgctggag
     2401 tacgcggcaa agccgcacgt gctcggtcgt ctgcgcgctt ccctggaggc gctcttgtga
     2461 aaacctccgt ccgcgctcgc ctggaggcca aggcccggga gcgcgggacc accttccact
     2521 acgcaagcct gctgtacatg caggagggct tcctggcccg gcttgcagcg tccccgcacg
     2581 cggacaagct catcctcaag ggaggcttcc tccttttctc gacgcgagcg cctatgggac
     2641 gcaccacccg ggacatcgac ttcctgggtg aggggctgcc gaacgaagag accagcctgg
     2701 tccgcatcgt agcgt
//
LOCUS       DASRZS010000184         3680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3561320, whole genome shotgun sequence.
ACCESSION   DASRZS010000184 DASRZS010000000
VERSION     DASRZS010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3561320"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /gene="mrdA"
                     /locus_tag="VFH83_04525"
     CDS             complement(<1..1535)
                     /gene="mrdA"
                     /locus_tag="VFH83_04525"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET6485660.1"
                     /translation="MQSAERLQQRRFMVLTGLFVAGIGLLVLYLFWLQIVRGFEYRQR
                     AKDVSQRETPIPAQRGEIYDRNADDPLVFNVDSFAVDVIPGDVPPAQLPALFARLAAL
                     LSMPVSQIESKVPPKTYHLYQPIEIKAGVQLDTISRLAEHIEDFTGVSWHNKPIRSYV
                     ESSTLAHVIGYVGDITTDELQVLYNKGYEPGTTLGKAGVEKEYDDILRGKDGTRYRVV
                     DVTERQLSSTQEDVVPPQPGRNVVLTIDRNIQKLAEQALGPRNGAVVVLKPTTGEVLA
                     LVSYPSFDPNRFYAPDSDAYLRQLSMDPSFPYIDRAIQSVYPPASTFKVIMTAGIIDD
                     GTIPINRAVLCTGKFVLGDRTAYCWVKTGHGYEDLFGGLAQSCDVYFWTTGYQLGPDK
                     ILAYARDFGVGSLTGIDLPEERSGLLPTPDWKEKVKHVAWVGGDTLNLAIGQGFVTFS
                     PLQMANMIAMVANQGVVYKPHLLKEITSSTGQVLSVTKPEVLHQSAISKETFRMVQDA
                     MRGV"
     gene            complement(1535..2050)
                     /gene="mreD"
                     /locus_tag="VFH83_04530"
     CDS             complement(1535..2050)
                     /gene="mreD"
                     /locus_tag="VFH83_04530"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET6485661.1"
                     /translation="MNRDLRVTVVTVVLLVVCVLLQSTILGRVALRGVRPDLALIVLV
                     FVSLRRGSMVGQVSGFVTGFLEDLMNVSPLGFHSLLRAVLGFLFGLLSGNVFVDPFLM
                     PMILTVVATILKGLLAGIVSAVFGVTASGFITFAGRLWIEVGYNGLLAPFLFALLGLF
                     RVFKQAEKEAA"
     gene            complement(2047..2937)
                     /gene="mreC"
                     /locus_tag="VFH83_04535"
     CDS             complement(2047..2937)
                     /gene="mreC"
                     /locus_tag="VFH83_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET6485662.1"
                     /translation="MPGLFEGFIARHRRGVTLAALVFVSLLCLAVSNRSIVVRPKEIG
                     LSVMGFFQKGVTGFFAWFGDTAGSVRQLGEARAELADATARLQQMDQVTRENVDLRRQ
                     NALLMNQLGFAQTLPPARIGAEVIARDTDNLFSTITINKGSRGGVQKGMTVVAYQQDL
                     EGLVGKVVAVGPGSSEILPLYDPQCMVSARLDRTRDEGLVSGEGKDSGTVLMRYVKSI
                     AKDAIEYGDLVVTSGLGGLYPKGINIGRIRDIRAQAYETSLEIEVEPVINFDRLEYVF
                     LFAGSSPVDQAPAATGAALP"
     gene            complement(2942..>3680)
                     /locus_tag="VFH83_04540"
     CDS             complement(2942..>3680)
                     /locus_tag="VFH83_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET6485663.1"
                     /translation="FVKPRMVIGVPTCITEVEKRAVRDSAEQAGARDVYLIQESMAAA
                     IGANIPILEPAGHMICDIGGGTTEISVISLGGMVVTNAIRVGGDEFDEAIIKHVRNVH
                     NLIVGESTAENVKITIGNASPDKKIEKIEIKGTDAITGLPRKLEMDSVEVREALQEPI
                     NTVIEEIKKTLGQTPPELAADIVERGIVMTGGGSLLKGFPKLISKETGVPVILAENPL
                     LCVALGAGKFLESLVKEKNSKYFNVKT"
BASE COUNT          652 a         1212 c         1180 g          636 t
ORIGIN      
        1 acgccgcgca tggcgtcctg caccatccgg aacgtctcct tgctgatggc cgactggtgg
       61 aggacctccg gcttcgtcac ggacagcacc tggcccgtgg aggaagtgat ctccttcagg
      121 aggtgcggct tgtagaccac cccctggttg gccaccatgg cgatcatgtt ggccatctgg
      181 agcggagaga acgtcacgaa accctgcccg atggccaggt tgagggtgtc cccgccgacc
      241 caggccacgt gcttgacctt ctccttccag tccggcgttg ggagaagccc cgagcgttcc
      301 tcggggagat cgatgccggt caacgagccg accccgaagt cccgcgcata cgccaggatc
      361 ttgtcgggtc cgagctggta cccggtggtc cagaaataga cgtcgcagga ctgagccagg
      421 cccccgaaca ggtcctcgta cccgtgcccg gtcttgaccc agcagtacgc cgtgcggtcc
      481 cccaggacga acttgccggt gcagaggacg gcccggttga tgggaatggt tccgtcgtca
      541 atgatgccgg ccgtcatgat gaccttgaag gtggacgcgg gcggatagac ggattggatg
      601 gcccgatcga tgtacgggaa ggacgggtcc atcgagagct ggcggagata cgcgtcgctg
      661 tccggagcgt agaaccgatt gggatcgaag gatgggtagc tcaccagggc gagcacctcc
      721 cccgtggtgg gtttgaggac caccaccgcg ccgttgcgcg gccccagcgc ctgctccgcg
      781 agcttctgga tgttgcggtc gatggtcagg acgacgttgc gccccggctg cggaggcacc
      841 acgtcctcct gggtgctgga gagctggcgc tccgtgacgt ccaccacccg atagcgggtg
      901 ccgtccttcc cgcggaggat atcgtcgtac tccttctcga ccccggcttt ccccagcgtg
      961 gtaccgggct catagccctt gttgtaaagg acctggagct cgtccgtggt gatgtccccg
     1021 acgtagccga tcacgtgagc cagagtgctg gactccacgt agcttcggat gggcttgttg
     1081 tgccagctca cgccggtgaa gtcctcgatg tgctcggcca ggcgggaaat cgtgtcgagc
     1141 tgcacgcccg ccttgatctc gatcggctgg tacagatggt aggtcttggg cggaaccttc
     1201 gactcgatct gcgatacggg catggagagc agggcggcca gacgcgcgaa gagcgccggt
     1261 agctgcgccg gcgggacgtc ccccgggatc acgtccaccg cgaaggagtc cacgttgaag
     1321 accaggggat catcggcgtt ccgatcgtag atctccccgc gctgcgcggg gatgggcgtc
     1381 tcccgctggg agacgtcctt ggcgcgctgt cggtactcga atccccggac gatctgcagc
     1441 cagaacaggt acagaaccag gaggccgatc ccggccacga agagtcccgt cagcaccatg
     1501 aaacgccgct gctgcaggcg ttcggcgctt tgcatcaagc cgcttctttc tccgcctgct
     1561 tgaacactcg gaacagcccc agaagcgcaa acaggaacgg tgccaggagc ccgttgtacc
     1621 ccacctcgat ccacagcctg cccgcgaagg tgatgaaccc ggacgcggtc acgccgaaca
     1681 ccgcggaaac gatgccggcc agaaggccct tcaggatggt agccaccacc gtcaggatca
     1741 tgggcatgag gaacgggtcg acgaatacgt tgcccgagag aagcccgaag aggaacccga
     1801 gcaccgcccg cagcagggaa tggaacccca gcggcgagac gttcatgagg tcctcgagga
     1861 agcccgtcac gaaccccgat acctgcccca ccatggagcc gcgccgcagc gacacgaaga
     1921 ccaggacgat gagcgccagg tccggacgga cgccgcgcag cgcgaccctt cccaggatcg
     1981 tggactgcag caggacgcag accaccagga gcaccacggt aaccacggtg acgcgaaggt
     2041 cgcggttcat ggcagcgccg ctcccgttgc cgccggagcc tggtcgaccg gcgaggagcc
     2101 ggcaaagagg aacacgtact ccagtcgatc gaagttgatc accggctcca cctcgatctc
     2161 cagcgacgtc tcatacgcct gggcccggat atcccggata cggccgatgt tgatcccctt
     2221 cgggtatagc ccacccagcc cggaggtgac caccaggtcc ccatactcga tggcatcctt
     2281 ggcgatgctc ttgacgtacc gcatgaggac cgtgccgctg tccttccctt ccccgctgac
     2341 cagaccctcg tcccgggtcc ggtccagccg cgcggagacc atgcactggg gatcgtacag
     2401 gggcaggatc tccgaagagc ccggtcccac cgccaccacc ttgcccacca gcccctccag
     2461 gtcctgctgg taggccacca ccgtcatccc cttctggacc ccgcctcggg atcccttgtt
     2521 gatggtgatg gttgagaaga ggttgtccgt gtcgcgggca atcacctccg cgccgatccg
     2581 cgccggaggc agggtctggg cgaagccgag ctggttcatg aggagggcgt tctgcctacg
     2641 caggtccacg ttctcccgcg tgacctggtc catctgctgg agacgggcgg tggcgtccgc
     2701 cagctcggcg cgcgcctccc cgagctgacg gacggagccg gccgtgtctc cgaaccaggc
     2761 gaagaagccg gtcacgcctt tctgaaagaa gcccatgacc gagagcccga tctccttggg
     2821 ccgtaccacg atgctccggt tggacaccgc caggcagaga agggagacga acaccagggc
     2881 agccagggtg acgcctcggc gatgacgcgc gatgaagcct tcgaacagcc cgggcatgag
     2941 atcaggtctt cacgttgaag tacttggagt tcttttcctt cacgaggctt tccaggaact
     3001 tgccggctcc gagcgccacg cagaggagag ggttctccgc aaggatgacg gggactccgg
     3061 tctccttgga gatcagcttg gggaaaccct tgaggagcga cccgccgccc gtcatcacga
     3121 tcccgcgctc cacgatgtcc gcggcaagct cgggcggggt ctgacccagg gtcttcttga
     3181 tctcctcgat cacggtgttg atcggctcct ggagcgcttc ccgcacctcc acggaatcca
     3241 tctcgagctt tcggggcagc ccggtgatcg cgtcggtgcc cttgatctcg atcttctcga
     3301 tcttcttgtc cggcgaagcg ttgccgatgg tgatcttcac gttctccgcg gtggactctc
     3361 ccacgatgag gttgtgcacg ttgcgaacgt gcttgatgat ggcttcgtcg aactcgtccc
     3421 cgcccacgcg gatggcgttg gtcaccacca tgccgcccag ggagatcacg gagatctccg
     3481 tggtcccgcc gccgatgtcg cagatcatgt ggcccgcggg ctccaggatg gggatgttgg
     3541 cgccgatggc ggcggccatg gactcctgga tgaggtacac gtcacgggcc cccgcctgct
     3601 ccgcggagtc ccgcacggcg cgcttctcca cctccgtgat gcaggtcggg acaccgatga
     3661 ccatgcgggg cttcacgaac
//
LOCUS       DASRZS010000185         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3946942, whole genome shotgun sequence.
ACCESSION   DASRZS010000185 DASRZS010000000
VERSION     DASRZS010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3946942"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFH83_04545"
     CDS             <1..600
                     /locus_tag="VFH83_04545"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET6485664.1"
                     /translation="LTYHHVPLSLRGVKIVITNSKVPHGLVDSEYNKRREECGRCVEL
                     LSAKKPGTALRDYTPADVRESMGVIPEATRKKCLHVVEENQRVLEAEDALRRKDLIAF
                     GKLMNRSHESLRDLYEVSCPELDWLVKRAWETEGVYGSRLTGAGFGGCTVTLIDEDAI
                     PRYREHISPYERIFGFHPELYICKPSAGARIMENRMQRA"
     gene            597..1340
                     /gene="surE"
                     /locus_tag="VFH83_04550"
     CDS             597..1340
                     /gene="surE"
                     /locus_tag="VFH83_04550"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET6485665.1"
                     /translation="MRILLTNDDGVGSDGIQALLRVLSPGNDVWVVAPESQKSGGSHS
                     ITLRDSIRVHRLDEQVYACRGTPADCVMVALLGLAPRGIDLVVSGINQGPNLGTDILY
                     SGTAAGARQGALMGVPSVALSMGGYGPPFDYEGAARFARRNLCLFHELGTDDHFLNVN
                     VPMEGADTASTVITFPSRRIYRHELHTYAAPDGDIYCFLGGDLPDAHPEEGSDCMELS
                     RGHISISPIHAHPRNWASIEARYREVVFQ"
     gene            1337..2032
                     /locus_tag="VFH83_04555"
     CDS             1337..2032
                     /locus_tag="VFH83_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6485666.1"
                     /translation="MNEKLEHAVKLYHSHRFEQAMAELKEMEAEGGDNAELDYYIGLT
                     LTQLGKYEDALLALEKVVASHFSFLHITQARMVLGYIYSVTGRFRLAEFEFAKVNELG
                     VQSSQAMAALGYVLYAQKKVRESVEMLNRALEIEPKNANALNSLGFIYAEEKMEPDKA
                     VYLCRQAVDLAPRNAAYLDSLGWAQFRQTNYVDARANLRRALDLAPGNKEIASHLRAV
                     MDALKEVSTPIRG"
     gene            2129..2290
                     /locus_tag="VFH83_04560"
     CDS             2129..2290
                     /locus_tag="VFH83_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485667.1"
                     /translation="MKIESVALAGTQNATSADLPIMKVEAIKEILYLGIRGDIKLDAG
                     KSHTVDTYA"
     gene            2309..>3260
                     /locus_tag="VFH83_04565"
     CDS             2309..>3260
                     /locus_tag="VFH83_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HET6485668.1"
                     /translation="MKRATPLWGLLLLAPALLSAQKIDFDAARSADQLRLGVQAFHRG
                     AFGDAITALEKSISYQPGNVLAQTWLGRSQFRAGFEAEAQRTWQQIVDGGQGTPLLRD
                     WISVLALRRGLGRELSGDARWVVSTQLDGMQKGGYPFRRPTSVRPRPDGSFWVVAFGS
                     DEVIHFDANYRLIATYRGGVQAFDRPYDVAEAPDGTLFVSEYGANRVAKCSASGQRIK
                     TFGTTGRGDGMLLGPQYLALDGRGYLWVTDWGNGRVERFDLDGNYVQAVSGIDGPTGV
                     AVHNDRLYVSERSKGRILVYDLSGNALGSIGGGTLQAPEGI"
BASE COUNT          583 a         1056 c         1071 g          550 t
ORIGIN      
        1 ctgacctatc accacgtgcc tctttccctg cgcggcgtga agatcgtgat cacgaactcc
       61 aaggttccgc acgggctggt ggactcggag tacaacaagc gccgggagga atgcggccgg
      121 tgcgtggagc tcctctccgc caagaagccc ggtacggccc ttcgggacta tacgccggcg
      181 gacgtgcggg agagcatggg ggtgatcccc gaggcgactc gcaagaagtg cctccacgtg
      241 gtggaggaga accagcgcgt gctggaggcg gaggacgcgc ttcgccgcaa ggatctgatc
      301 gccttcggca agctcatgaa ccgctcccac gagagccttc gggacctcta cgaggtctcc
      361 tgcccggagc tggactggct cgtgaaacgg gcgtgggaga cggagggagt ctatggctcc
      421 cggctcaccg gcgccgggtt cggcggatgc acggtcacct tgatcgacga ggacgccatc
      481 cctcgctatc gggagcacat ctcgccttac gagcgcatct tcggtttcca cccggagctg
      541 tacatctgca agccgtcggc gggcgcccgg atcatggaaa acaggatgca gcgggcatga
      601 ggatcctgct caccaacgac gacggcgtgg gcagcgacgg gatccaggct cttctccgag
      661 tgctctcccc cggcaacgac gtctgggttg tcgcgcccga aagccagaag agcggcggct
      721 cgcactccat cacgttgaga gactcgatcc gtgtccatcg cctggatgag caggtgtacg
      781 cctgtcgcgg cacgcccgcg gactgcgtca tggtggcgct gttggggctg gccccccggg
      841 gaatcgacct cgtggtgtcc ggcatcaacc agggtcccaa cctgggaacc gacatcctgt
      901 actccggcac cgcggcgggg gcccgccagg gagccctcat gggggtgccc tccgtggcgc
      961 tctccatggg cggctacggc ccgcccttcg actacgaggg agccgcgcgg tttgcccgcc
     1021 gcaacctgtg cctctttcat gagctgggca ccgatgatca cttcctgaac gtcaacgtcc
     1081 cgatggaggg cgccgatacc gccagcacgg tgatcacgtt tccgtcccgc aggatctatc
     1141 ggcacgagct tcacacctat gcggcgccgg acggcgatat ctattgcttt ctgggcgggg
     1201 atctccccga tgcccatccc gaggagggct ctgactgcat ggagctttcc cggggccaca
     1261 tctcgatctc tcccatccat gcgcacccgc gcaactgggc gtcgatcgag gcccggtacc
     1321 gggaggtggt gtttcaatga acgagaagct cgagcacgcc gtcaagctct atcactccca
     1381 tcgtttcgag caggccatgg cggagctcaa ggagatggag gcggagggcg gggacaacgc
     1441 ggagctggac tactacatcg ggctcaccct cacccagctc gggaagtacg aggacgccct
     1501 cctggccctg gagaaggtcg tggcgtccca cttcagtttt ctccatatca cgcaggcccg
     1561 catggtgctg ggatacatct actcggtcac gggccgcttc cgcctggcgg agttcgagtt
     1621 cgccaaggtg aacgagctcg gggtccagtc gtcccaggca atggcggctc tcggctacgt
     1681 gttgtacgcg cagaagaagg tgcgggagag cgtcgagatg ctgaacaggg ccctggagat
     1741 cgagccgaag aacgccaacg ctctcaactc cctcgggttc atctatgccg aggagaagat
     1801 ggaaccggac aaggccgtct acctctgcag gcaggccgtg gaccttgccc cccggaacgc
     1861 ggcgtacctg gactccctgg gatgggccca gttccggcag accaactacg tcgacgcgcg
     1921 cgccaacctt cgccgggcgc tggacctggc gccgggcaac aaggagatcg cctcccacct
     1981 gcgcgccgtc atggacgcgc tcaaggaagt ctccactccc attcgaggct gacggcgcgc
     2041 aacgcgtccg cgttccggcc gcggaccgcc gcaaaaaaaa ggtcatcgcg cctctcaaac
     2101 ccgtcgaaac tcattacagg agcaggtcat gaagattgag agcgttgctc ttgccgggac
     2161 ccagaacgcg acctccgccg accttcccat catgaaggtg gaggcgatca aggagatcct
     2221 gtatctgggc atccggggcg acatcaagct cgacgccgga aagagccata cggtagatac
     2281 atacgcctga ttctcctata ctcccgtcgt gaagcgtgcg accccgctct ggggacttct
     2341 ccttctggca cccgccctcc tgtccgccca gaagatcgat tttgacgctg cccgatccgc
     2401 ggaccagctt cgtctggggg tacaggcctt ccaccgcggc gccttcggcg atgccatcac
     2461 cgctctcgag aaatccatca gctatcagcc gggaaacgtc ctggcccaga cctggctggg
     2521 acgttcccag tttcgggccg ggttcgaggc ggaggctcag cgcacctggc agcagatcgt
     2581 agacggcggg caagggacgc ctctccttcg ggactggatc agcgtccttg cgctccgccg
     2641 gggactgggc cgagagctct cgggagacgc gagatgggtc gtcagcacgc agctggacgg
     2701 aatgcagaag ggaggctacc cgtttcgccg gcccaccagc gtgcgtccgc gcccggacgg
     2761 atcgttctgg gtcgtggcct tcggctccga cgaggtcatc cacttcgacg cgaactaccg
     2821 cctgatcgcg acgtatcgag gcggcgtcca ggccttcgac cgaccctacg acgtggcgga
     2881 ggccccggac gggaccctgt tcgtctcgga gtatggcgcc aatcgagtcg ccaaatgcag
     2941 cgcctccgga cagagaataa agacgttcgg gaccacgggt cggggagacg ggatgctcct
     3001 gggaccgcag tacctggcgc tggacggccg ggggtacctc tgggtgacgg actggggcaa
     3061 tgggcgggta gagcggttcg acctggacgg gaactacgtg caggccgtct ccggcatcga
     3121 cgggcctacg ggggtggcgg tccacaacga ccgtctctac gtttccgagc gctccaaggg
     3181 gcggatcctg gtctatgacc tgagcggcaa cgcgctggga tcgatcgggg gcggcacgct
     3241 gcaggcgccg gagggcatct
//
LOCUS       DASRZS010000186         8999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3180560, whole genome shotgun sequence.
ACCESSION   DASRZS010000186 DASRZS010000000
VERSION     DASRZS010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3180560"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..102
                     /locus_tag="VFH83_04570"
     CDS             1..102
                     /locus_tag="VFH83_04570"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET6485669.1"
                     /translation="MYKLTTERIRQIQKRAIKRLRHHSRSHALRVYL"
     gene            233..2017
                     /locus_tag="VFH83_04575"
     CDS             233..2017
                     /locus_tag="VFH83_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6485670.1"
                     /translation="MKRFLRRWPILLVAILLTAGGVVLAALQVQWARSAARAEEERLY
                     ASLTRGARQAAIEAEDEIGVIESMALSAAAGYSTQDWSRLTASLRLWQRYAPFPELLH
                     GLYVVQTGEPAQVFEYSRAESRLISAALPVNVQDALQGTLASGPERWRTTVLRGAYRI
                     TVVPALGNGLPSRPGRSVVAIVMVLDTRVLYEKILPRLVATDLGDCPFRIVAADARSI
                     IAQSPGLPAGRHPDVAISVTSAPFGGTTMPWESFARPPNGDLPQPQRLRAAPFDPFLQ
                     SWLQRVQGNASAARAIPEVVVPAEPEATLQIFYPGASIDATARLRQAVNVGVSLGILS
                     LLIASVFVLMRLYAGSLAVRAREQEFVASVTHELRTPLAVIQATSQNLTRGVVSDPSR
                     RLRYAEVIYDQVRRLSRMLEGVLLYAGFQSAKARPPALAEFDARVLIQDILQPLSHLA
                     SERGSILRLEFRDLPARIRSDRMALGIVLENLVVNGIRHADPGEVRVRVAVDEARILS
                     VVVEDDGPGIPFREQARIFGPFVRGERSVREQVPGSGLGLHLVQRVVTILGGTVRLQS
                     PYPIARGASHPGCRFQVTLPFEEVEHHD"
     gene            2010..2801
                     /locus_tag="VFH83_04580"
     CDS             2010..2801
                     /locus_tag="VFH83_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6485671.1"
                     /translation="MTERPRLSPNAGNARAAPAPTRGPASAHGARILVVEDEEGLVLA
                     LEDALRKEGFEVRSSMDGITGQAEAASGGIDLVILDVMLPGRDGFQVCQRLRAGGMSM
                     PILMLTARSTAIDTVMGLHLGADDYLSKPFDMAVLIARVNALLRRTGAGGASLRANGD
                     ADATVLWFGEFALDSLRMQLTRAGAVIPLNAQEYRLLEVFLRSPDRVLSRDTLLCEAW
                     GYNTETTTRTVDVHVGWLRQKLGEKNRPRHILTVRGVGYTFVSEP"
     gene            2903..3268
                     /locus_tag="VFH83_04585"
     CDS             2903..3268
                     /locus_tag="VFH83_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6485672.1"
                     /translation="MILVVDDEKSIRTLVTQLLQADGYAALSARDAQEALEIVRDSPS
                     DIALVITDFDLPGLNGVDLCTELNRRRPGIGLIVLSGEPREIPGGLPFIGKPFGRATL
                     MANVRHVLSAGMARDSASA"
     gene            4133..5350
                     /locus_tag="VFH83_04590"
     CDS             4133..5350
                     /locus_tag="VFH83_04590"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485673.1"
                     /translation="MRPGSASARECRRKILILTMGMGSGHNVPAASVAEAIARYHPSV
                     DAPVVDLADDARPRYGRLEKRCWRFCLRHPEILKPFYYATHRSIRFVRVLDTASRRKA
                     SSDVLDLLELERPDVILCTHYGPANIISRLRKDHPGLPPVVVLITDPFEPHALWLVEP
                     SELYLLSALGRAEELSAAGIPAERIRNIDFPLRLCFTSDTATAWKPRPAPLQGKNAKL
                     RVTLAAGGEGIGRLRAQVDALLKANLDLFLVVLCGRNREVREALLKLVQERRSARTTV
                     VPVGFTDDVHGILSATDITMGKSGTSFTLESLFCGKPFLITQTLANEEGCRDFVLRNG
                     AGWFEPTIPAQVELVRRLVTDRALLREVSRNCRALGLRNGSRQIADLLTRMARDRARF
                     GAVPPCPGPRAAH"
     gene            5558..7888
                     /locus_tag="VFH83_04595"
     CDS             5558..7888
                     /locus_tag="VFH83_04595"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6485674.1"
                     /translation="MVRKGSRSLFVRFFLSYTAVLLVPLAFATIGYLRLLGVLEHRTA
                     QEEYVRFTKYTELLDQRFRELEATATQLALSPSVRGLLDRDSPFVDGTVIPRILELQE
                     QLSSLALTNTFVVNVHVFLKRAETVVSSYHTFFGLPSYYGRYLSYGGLDIEQWTRLIY
                     QGPHQREYLPAQTVTVRTKQEESTTQRMVLYVQSLPMSAYSVPRGAIVAFLPEQEALG
                     YLGWTGPGSAAAYLIMDANGRPIAGDPLLASEGARASGAEATRAMVRGIRVLLSQVQS
                     PESHWRYLEAVPATVMSGREAPETLVMVALLLAALIAGVLLSSLVASRTSRPIANTLR
                     LLVGGEESSRSAVSRNELDFLQDSVVRLVESDRAFRQSLREQEPRFRIAFLERMLTGG
                     FETEEEARLYLSQTSLPPLPWPVRVLLVRINERRSPRDAEALSRITAAKAVVHSRVGS
                     YFSSHGYVHDADFDLVAVLVEESQWAGIGPFAASLLDMLAGEADTGFSFGVGEPAGSL
                     REIARAAREAREALAYKPSSGGVVFHEEIPTDSELYYPLDFAPKFISFTMAGDEEQIR
                     GLLEELRERNCVRRRASLAMQRQLEYAIRDTLTRLIDRIQERDPACAQGMRQTLFERF
                     DASTTMEELFGAATAVVSAACRTVNVAKKSHNVLLRDRILDCLNARYADPSVYLPSVA
                     EEFGITKEYLSQFFKEQTGENFSVYLERLRIRRAKDLLSETDWRLSRIARQCGYNSLP
                     VFRQAFKRLEGVTPSQFRAMAASHPGGARQPGPPPS"
     gene            8051..>8999
                     /locus_tag="VFH83_04600"
     CDS             8051..>8999
                     /locus_tag="VFH83_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6485675.1"
                     /translation="MSTGVDLSTMGLVGSREPAPGRRRGVNGVRLIRKHWQYYLLFAV
                     PLLYVIIFGYVPMGGVLLAFKTYRISDGILRSPWAGLRYLEQFFSSPRFWDLLRNTLS
                     LSVYSLVAGFPIPILLALMLNETNSLKFKKTVQMVTYAPYFISTVVMVSIIMQFLDPR
                     IGLANRVIVLLGGQARNFMGQESLFQSIYVWSGIWQNTGYAAIIYIAALSGIDPTLYE
                     AAYIDGASRLQKVIHIDLPGILPTIVILLILSIGSIMNVGFEKVFLMQNPLNLGSSEI
                     IATYVYKIGILSAQFSFGTAVGLFNSVVNLVLIASVNQVA"
BASE COUNT         1484 a         2942 c         2942 g         1631 t
ORIGIN      
        1 ctgtacaagc tgaccacgga gcgcatccgc cagatccaga agagggccat caagcgactc
       61 cggcatcatt cgcgcagcca tgcgctgcgc gtgtacctgt aggggccggg gccacgattc
      121 caccgactcg tgtaaaggcg gagtaaaaac tcggtaaaac cacgcaaaag ctcgccgttg
      181 tgggggctat ccggcttgca aatcagaggc agccgggtag attggcgccg tgatgaaaag
      241 attcctccgt cgctggccca tccttctcgt ggccattctt ctgacggcgg gaggcgttgt
      301 cctggccgcg ctccaggtcc agtgggcccg ctcggcggcg cgcgccgagg aggagcggct
      361 ctacgcctcc ttgacgcgag gcgcccgcca ggcggccatc gaagcggagg acgagatcgg
      421 cgtcatcgaa tccatggcgc tcagcgccgc agccggctat tcgacccagg actggagtcg
      481 gctgacggct agtctgcggc tgtggcagcg gtatgcgccg tttccggagc tgttgcacgg
      541 tctgtacgtc gtgcagacgg gcgaacctgc gcaggtgttc gaatactcgc gcgccgaatc
      601 ccgtctgatc tctgccgctc tgccggtgaa cgtccaggat gcactgcaag ggacgctggc
      661 atctggaccc gagcggtgga ggaccacggt actcaggggc gcgtaccgga tcaccgtggt
      721 gccggccctg ggcaacggtc ttccttcccg acccgggcgg tcggtcgtgg cgatcgtgat
      781 ggtgctggat acgcgggttc tctatgagaa aatcctgccg cggttggtgg cgaccgatct
      841 gggggattgt ccgttcagga tcgtcgccgc ggacgcgagg tcgatcattg ctcagtcccc
      901 tggtctgccg gctggccgcc atcccgacgt cgcgataagc gtgaccagcg cgccgtttgg
      961 cggcaccacc atgccttggg agtcgttcgc gagaccgccc aatggtgatc ttccccaacc
     1021 tcaacggctg cgggccgcgc cgttcgatcc ctttctccag tcgtggctgc agagagtcca
     1081 gggaaatgcg agcgccgctc gcgcgatccc cgaggtcgtc gttcccgccg agccggaggc
     1141 gaccctccag atcttctatc ccggagccag catcgacgcc acggccagac ttcgccaggc
     1201 cgtgaacgtg ggtgtgagcc tcggcatcct ctcccttctc atcgcgagcg tcttcgtgct
     1261 catgcgcctc tatgcgggca gtctcgcggt gagagcgcgc gagcaggagt ttgtggcctc
     1321 cgtcacccac gagctgcgca cgccgctcgc cgtcatccag gccacctccc agaacctcac
     1381 ccggggagtg gtcagcgacc catcccggcg actgcggtac gcggaggtga tctacgatca
     1441 ggtccgccgg ctctcgcgca tgctggaagg cgtgcttctg tatgcgggat tccagtcggc
     1501 aaaggcgcgg ccgcccgccc tggcggagtt cgatgcgcgc gtcctgatcc aggacatcct
     1561 gcagcctctc tcccatctcg cctccgagcg cggatctatt ctgaggcttg agttccggga
     1621 cctgccggcg cgcattcgct ccgaccgcat ggctctgggc atcgtcctgg agaacctcgt
     1681 cgtgaacggg atccgccacg ccgaccccgg agaagtccgc gtccgggttg cggtggatga
     1741 ggcccggatt ctctctgttg tcgtcgagga cgacggcccg ggcatcccgt tccgcgaaca
     1801 ggctcgaatc ttcgggcctt tcgtgcgcgg cgagcgcagc gtgcgcgagc aggtgccggg
     1861 cagcgggctg gggctccatc tcgttcagcg ggtcgtgacg atccttggag ggaccgtccg
     1921 cctccagagt ccgtatccga tagctcgcgg cgcgagccac ccgggctgtc gcttccaggt
     1981 cacgctgccc ttcgaagagg tggagcatca tgactgaacg gccgcgcctg tcgccgaatg
     2041 ctgggaacgc gcgcgcggcg ccggccccca cgcggggacc ggcctccgcg catggcgccc
     2101 ggatcctcgt ggtagaagac gaggaggggc tcgtgctcgc cctcgaggat gcgctccgca
     2161 aggagggatt cgaggtgcgg tcctccatgg acggcatcac cggccaggcc gaggcagcaa
     2221 gcggcggaat cgacctcgtg atcctcgacg tcatgcttcc cggccgcgac gggttccagg
     2281 tgtgtcagag gctgcgggcg ggcgggatgt cgatgcccat cctgatgctc accgcgcgca
     2341 gcaccgcgat tgacacggtc atggggctgc atctcggggc ggacgactat ctgagcaagc
     2401 ccttcgacat ggccgtcctg atcgctcgcg tcaacgctct cctgcggcgc accggggccg
     2461 gcggagcgtc gttgcgggcg aacggcgatg ccgacgcaac ggtcctctgg tttggggagt
     2521 ttgccctcga ttccctgcga atgcagctca cgcgggcagg cgccgtgatc ccgctcaacg
     2581 cgcaggagta ccgccttctg gaggttttct tgcgcagccc ggaccgcgtg ctgagccggg
     2641 acacgctgct ctgcgaagcc tggggctaca acaccgaaac gacgacgcgg accgtggacg
     2701 tgcacgtggg gtggctgcgc cagaagctcg gagagaagaa tcgaccgcgc cacatcctga
     2761 cggtccgtgg cgttggatac accttcgtgt ccgagccctg aagcgctccg atcctggatt
     2821 ggaatcgaag ccgcgaagga ggcgcgtctt gcagcctgca catcgacatc atgcgtccga
     2881 cggcagtggc tcaccccacg cggtgatact ggtagtcgac gatgaaaaga gcattcggac
     2941 cctggtcacg caactcctac aggcggatgg ctatgcggcc ctttccgcgc gcgacgcgca
     3001 ggaagccctc gagatcgttc gtgatagtcc gagcgacatc gcgctggtga tcacggactt
     3061 cgatctgccg gggttgaatg gcgtggatct ctgcacggag ctgaatcggc ggcgacctgg
     3121 gatcggactg atcgtcctgt cgggggagcc gcgggagatc ccgggcggcc tgccgttcat
     3181 cgggaaaccg tttgggaggg cgacgctgat ggcgaacgtc cgccatgtcc tgtcggcggg
     3241 gatggcacgg gattccgcgt cagcctgagg cgtactttac accggcttta cacccatttt
     3301 gcacgagttt agccccggct tcatcctcca cctggacgga agcgctaccc tgggatcgag
     3361 attgagaggg acgacagacg tggaacgcgg aagccgacac gacgcgacag taggacgaag
     3421 cttcggcccg gggcaaagtc tcggggcacg gagtgagggt cacgatcctc ctcctcatca
     3481 cttcccattg cgcacctcgc gccgctcaag gagggcggcg gtatcgcaga ggtccaacca
     3541 tgtcgggacg tgaccctcac tcagtgcttc gaggcgtcgt cccgtcggcc ctgtgcctgg
     3601 tcgtgtccgg ttcgtgcgcg tcgctgcccg gccgccgaga aggggagggc gggatctcac
     3661 gcaacgagac gctctacctg acgccgggcc agagctcccg gatcatccca atcctgcggg
     3721 cgctgaagca tcggctgacc gtgtcggggt ccggcctttg ggaagcacca ctggccgttg
     3781 atgccgagct cacccgcgag cagagggccg actgcaagcg gtacgcctca gcgctcgggg
     3841 agccaccgcg cggcgccggg cagtcatcgg gacctcgcgg agggtgggga gcccttggag
     3901 gtccggggtg tggcggagga cttgggcggg gcggtggaat gaatcctgga gcgggaccgg
     3961 gtcccggtgg gcaggggccc ggcggtgaga cagagccacc tccgccccat gcggatgagt
     4021 cggccatccg ctcggagcgg ttggagcgtc aggcccgaga ggtggaagcc ttcatcgcgg
     4081 tactccagag cggcctcgat cggatcgcct cggcatcggc aacggtgggc tcatgagacc
     4141 cggatccgcg tccgcccggg aatgccgcag gaagatcttg atcctcacga tgggaatggg
     4201 atccggacac aacgttcccg cggcttccgt ggccgaggct atcgcccgat accatccgtc
     4261 cgtagacgcc ccggtcgtgg acctggcaga cgacgcccgg ccccggtacg gccggctcga
     4321 gaagcggtgc tggaggttct gcctgaggca tccggagatc ctcaagccgt tctactacgc
     4381 aacccatcgg agcatccgat tcgttcgcgt gctggatacc gcgtcgagac ggaaggcgtc
     4441 ctctgacgtc ctggatctgc tggagttgga gcgccctgac gtcatcctgt gcacgcatta
     4501 cggaccggcg aacatcatct cccgattgcg aaaggaccat cccggcctgc caccggtggt
     4561 cgtgctgatc acggacccgt tcgagcccca tgcgctctgg ctggtggaac cgtccgaact
     4621 gtacctgctg tccgccttgg ggagggcgga ggagttgagc gccgctggga taccggccga
     4681 acgcatccgc aacatcgact tcccgttgcg gctgtgtttc acctccgaca cggcgaccgc
     4741 ctggaagccg cgccccgcac ccctccaggg gaaaaacgcc aagctccgtg tcacgctggc
     4801 cgccggcggc gaggggatcg ggaggctgcg ggcccaggtg gacgcgctgc tgaaggccaa
     4861 cctcgacctc ttcctggtcg tgctctgtgg ccgcaaccgg gaggtacggg aggcgctgct
     4921 gaagctcgtc caggaacgca gatcagcccg gaccacggtg gtaccggtcg gctttaccga
     4981 tgacgtgcac ggtatcctgt ccgcaaccga catcaccatg ggcaagtcgg gcacctcatt
     5041 cacgctggag tctctgtttt gcggaaagcc gttcctgatc acgcagacct tggccaatga
     5101 ggagggatgc cgggacttcg tgttgcgaaa cggcgccggc tggttcgagc ccaccatacc
     5161 tgctcaggtc gagctggtga gacgactcgt tacggatcga gcgctcctgc gtgaggtcag
     5221 ccgcaactgc agggcgcttg ggctgcgcaa cggctctcgg cagatcgccg accttctcac
     5281 ccgcatggcg cgcgataggg cgaggttcgg ggccgttcct ccgtgccctg gcccgcgcgc
     5341 agcgcattga ggtcgcccag ccgctacgag agctcctcca accgggccgg gatccccgtc
     5401 gagtcgttcc agggcgatcg cccgcgcaga tcatgtgtct gggccacgca ccgtatcctg
     5461 gccgcatggg cttcatgtcc caggccggtc accgcaccga aggcctggac taccaggggt
     5521 tccggacgcg tgctacactc gccgggggac actcgccatg gtcaggaagg gctctcgatc
     5581 gctgttcgta cggttcttcc tctcctatac cgcggtcctt ctggtacccc tggcatttgc
     5641 aaccatcggg taccttcgcc ttctgggagt cctggagcat cgaacggctc aggaggagta
     5701 cgtccgcttc acgaagtaca ccgagttgct ggaccagcgc tttcgcgagc tggaggcgac
     5761 ggccacccag ctcgcgctca gcccgagcgt acgaggtctc ctggatcggg acagcccctt
     5821 tgtcgacggg accgtcatcc cgcgaatcct ggagcttcaa gagcagcttt cctccctggc
     5881 gctcaccaac acgttcgtgg tcaacgtcca cgtattcctc aagcgcgcag agacggtggt
     5941 gagctcctac cacaccttct tcggcttgcc ctcctactat ggcaggtacc tcagctacgg
     6001 aggactcgat atcgagcagt ggacacggct catctaccag ggcccgcacc aacgggagta
     6061 cctgcctgcc caaacggtca ccgtgaggac caaacaggag gagagtacca cgcaacggat
     6121 ggtcctttac gtgcagtctc ttccgatgag cgcctacagc gttccgcgcg gtgcaatcgt
     6181 tgcgttcctg cccgaacagg aggccctggg gtacttgggg tggaccggtc cggggtccgc
     6241 cgccgcctat ctgatcatgg acgcgaacgg acgccccata gccggcgacc cgctgctggc
     6301 cagcgaaggt gcccgtgcct ctggggcgga ggcaacccgg gcgatggtgc gcggcatccg
     6361 agtcctgctc agccaggtgc agtcgccgga gtcccactgg aggtacctgg aagctgtacc
     6421 ggccacggtc atgagcggac gtgaggctcc tgagaccctc gtcatggtcg cgctgctcct
     6481 tgcggcgctc atcgcgggcg tgctgctgtc ttccctggtc gcctcccgga ccagccggcc
     6541 catcgccaac acgcttcggc tcctggtggg gggagaggag tcctcccgaa gcgccgtgag
     6601 tcgaaacgag ctggacttcc ttcaggactc ggtggttcga cttgtggaga gcgaccgcgc
     6661 tttccggcag agccttcgag agcaggagcc ccggtttcgc atcgctttct tggagcggat
     6721 gctcaccgga gggttcgaga cggaggagga agcccggctc tatctctccc agaccagtct
     6781 cccacccctg ccatggccgg ttcgcgtgct actggtgcgg atcaacgagc ggcgttcccc
     6841 gcgtgacgcg gaggcgctgt cccgcatcac ggcggccaag gcggtcgtcc acagccgcgt
     6901 gggctcgtac ttctcctccc atgggtacgt acacgacgcg gacttcgacc tggtggcggt
     6961 cctggtggag gagtcccaat gggctggcat cggtcccttt gccgcgtcgc ttctggacat
     7021 gctggccggg gaggccgaca ccggcttctc cttcggagtc ggagagcctg cgggaagcct
     7081 tcgggagatc gctcgcgcgg cccgcgaggc gcgcgaggcg ttggcctaca agccgtcgtc
     7141 tggcggggtc gtgttccacg aggagatccc caccgacagc gagctgtact atccgctgga
     7201 cttcgcgccc aagttcatct ccttcacgat ggcgggagac gaggagcaga tccgtggcct
     7261 cctggaagag ttgcgggaga gaaactgcgt gcggcgccgg gcttcgctgg ccatgcagcg
     7321 ccaactggaa tacgccatcc gtgacaccct taccaggctc atcgatcgga tccaggagcg
     7381 ggatcccgcc tgcgcccagg ggatgcggca gacgctgttc gagcgatttg acgcgtccac
     7441 caccatggag gagttgttcg gagcggcaac cgcggtcgtc tcggctgcct gcaggaccgt
     7501 caacgtggcc aagaagagcc acaatgtgtt gctgagggat cggatcctgg actgtctgaa
     7561 cgcgcgctac gccgatccat cagtgtacct gccgtcggta gcggaagagt tcggcatcac
     7621 caaggagtat ctgtcccagt tcttcaagga gcagaccgga gagaactttt ccgtatacct
     7681 ggagcggctt cgcatccgtc gggctaagga cctgctgtcc gaaacggact ggaggctctc
     7741 gcggattgcc cgccaatgcg gatacaacag cctccccgtc ttccgtcagg cgttcaaacg
     7801 tctggagggt gtcacgccgt cccaattccg agccatggcc gcatcccacc cgggcggtgc
     7861 ccgccagccc ggacctcccc cttcttaaca gatcctcctc ggccttaagg actcgacccc
     7921 ctcgggcggc gaattctcaa ccggagttcc ctctccttaa gaaccggctc tttacggagg
     7981 gccgtgcgtg gtccacggtt gggacggtcg ccgagaaggg ccgacctgga gtccctgcaa
     8041 ggagccgcga gtgtcaacgg gtgtggacct gagcacgatg ggcttggtgg gcagccgcga
     8101 accggccccg gggcgtcgca gaggggtgaa cggcgtcagg ctgattcgca aacactggca
     8161 gtactacctg ctgttcgccg tcccgctcct atacgtcatc atcttcgggt acgtgcccat
     8221 gggcggcgtt ctcctggcgt tcaagaccta ccgcatctcc gacggcatcc tccgaagccc
     8281 ctgggcgggg ctccggtacc tggagcagtt cttctcgtcg ccacggttct gggacctgtt
     8341 gaggaacacc ctgagtctca gcgtctactc cctggtggcc ggctttccta tccccatcct
     8401 gctggcgctc atgctgaacg agaccaacag cttgaagttc aagaagaccg tgcagatggt
     8461 gacgtacgcg ccctacttca tctccacggt ggtcatggtc tccatcatca tgcagttcct
     8521 cgatccgcgc atcggactgg ccaaccgggt gatcgtgctc ctcggaggcc aggcgcgcaa
     8581 cttcatgggc caggagagcc tgttccagtc catctacgtc tggtcgggca tctggcagaa
     8641 caccggctac gcggccatca tctatatcgc cgccctctcc ggcatcgatc ccaccctcta
     8701 cgaggcggcc tacatcgatg gtgcctcgcg cctgcagaag gtgatccaca tcgacctgcc
     8761 ggggatcctg cctaccatcg tgatcctcct gatcctgagc atcggcagca tcatgaacgt
     8821 gggcttcgag aaagtcttcc tcatgcagaa cccgctgaac cttggcagct cggagatcat
     8881 cgccacctat gtatacaaga tcgggattct cagtgcgcag ttcagcttcg gcaccgccgt
     8941 gggactgttc aacagcgtgg tgaacctggt cctgatcgcg tcggtcaacc aggtcgccc
//
LOCUS       DASRZS010000187         4750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3947545, whole genome shotgun sequence.
ACCESSION   DASRZS010000187 DASRZS010000000
VERSION     DASRZS010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3947545"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VFH83_04605"
     CDS             <1..900
                     /locus_tag="VFH83_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485676.1"
                     /translation="SLLDINSLYQKGYADIGDTTGSDEGRKAKMSDGKVFSCLGYANL
                     ISDVQATLSKDNDERRFVMFYLTKDPSVKYVYMNGAWTDSAAGIHLNSKLSDERTARA
                     MRFLDYLMGPEGSLLVCSGVEGISYSKDPKTGWYTPTPEVFNGYRTWDGSILRKTGVG
                     GWTNVLPNIAGLNEQGHAWDINAEYGFLQDKWVIYNNADWKSFAFNWPITPYSELDSD
                     TQAAALDASSKIGAYADDRLVNIALSKSPDVLKSEYAKFVSQMKTDGLDAFEAAWTDN
                     WKAYAKSKGRAPEHFFVTVDEAK"
     gene            919..1860
                     /locus_tag="VFH83_04610"
     CDS             919..1860
                     /locus_tag="VFH83_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6485677.1"
                     /translation="MSGGVEEARGRRPGRGRRFLSQWRFQLVLVPVTIWALVFFYLPA
                     AGNIIAFQDYRLDKGYLRSSFVGLKHFADFLQNQLTLVLLRNTLAMSFLGLISGTVAA
                     IVFAILLNEIVRSAPKRVVQTVSYLPYFVSMAICANLFIQLLSRTGPINQMLLSAGLS
                     DKPIPFLEIGPLFWWILTLQGVWKNVGWNAIIYIAAITSIDPQIFEAAYADGAGRTRR
                     IWHITLPLLLPTVAILLILNSGYLIMGGFEQQLLMFNPVVMDYAEVLQTYVYKRGIGH
                     QQYSFAAAVNLFQSVVSVTIILVVNRLFKRFTESSIW"
     gene            1868..2758
                     /locus_tag="VFH83_04615"
     CDS             1868..2758
                     /locus_tag="VFH83_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485678.1"
                     /translation="MNQAQASATIGEKAFRVFLFVFFLLFSVTVVYPFVYLLALSFNE
                     GTDALRGGITILPRMFTVENYRIIFENPQLLHGVLNSFLRTVIGACSATFFSGFLGYT
                     FTKRKLVGYRFYITILVIPMYFTAGIIPTFLVYRSLGLINNFLVYIVPNLVWAYNIII
                     MRTFISSLPQALEESAFIDGANEYTIFFRIILPLSLPAFATIVLFDAVWHWNSWFDTI
                     LYAPREGLETLMSILARMLLELRAREIAGLKASKHAMNITSETLRAAMTIATTLPIVL
                     VYPFLQKYFVKGVLIGAVKG"
     gene            complement(2783..3211)
                     /locus_tag="VFH83_04620"
     CDS             complement(2783..3211)
                     /locus_tag="VFH83_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET6485679.1"
                     /translation="MRGRLRQSRAARDRPISGINITPFTDVVLVLLIIFMIATPALVR
                     TGITVNLPKVTSADTQQPQTVTISIDTVGNVYLNQAKVALTDLRNQITAMIKGRTDVP
                     VVILGDKDVKYDVVVQVLQIVKAAGINHLSLAVDLTKTTP"
     gene            complement(3201..3821)
                     /locus_tag="VFH83_04625"
     CDS             complement(3201..3821)
                     /locus_tag="VFH83_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET6485680.1"
                     /translation="MFAGKTFADILAMGGPTMYVLLFCSVLSIAVIIERLLNYGAKAR
                     VKRAVFMQSIREELGRKDIKKAIELCNDCEAPFAKVALAGLQRAGHTERFVAGVMERQ
                     TTLEEVKLERLTGVVGTIGNTAVYIGLFGTVLGVIRAFQDISAAGMGSMNVVIGGVAE
                     ALITTATGLAVAVPAVIFYNYFTRRIEAFVTDMELCASEVADLVAR"
     gene            complement(3850..4680)
                     /locus_tag="VFH83_04630"
     CDS             complement(3850..4680)
                     /locus_tag="VFH83_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET6485681.1"
                     /translation="MYRCVSPGAIGIDLSWEACLPLARDSGFEGIDVPLEEGIPGERY
                     REGLSKFHLKPGGTGFPVRVSEDRTAFEAQISKLPAIAARAQAAGVTRFATWILSWSD
                     TLPYRENFKLHAAQLGQAARILQDHGCRLALEFLGPRSLREGHRYTFAHTIEQMLELG
                     DAVGKNVGLLLDAYHWYTSVSTLDQLLELENRQVVYVHINDAPGVPVEKQQDQVRRIA
                     GEGVAIDLPGFLSALRKIGYDGPVVPEPFVPELAQMPPDKAMARVAEGLKRVWDAPAR
                     "
BASE COUNT          857 a         1566 c         1416 g          911 t
ORIGIN      
        1 agcctcctgg acatcaactc cctctaccag aaggggtatg ccgacatcgg agataccacg
       61 ggcagcgacg aggggcgcaa ggccaagatg tccgacggca aggtcttcag ctgtcttggc
      121 tacgccaacc tgatctccga cgtccaggct accctgtcca aggacaacga cgagagacgg
      181 ttcgtgatgt tctacctgac caaggatccg tcggtgaagt acgtgtacat gaacggagcc
      241 tggacggact ccgcggccgg gatccacctc aactccaagc tgagcgatga acgcaccgcg
      301 cgggcaatga gattcctgga ctacctgatg gggccggagg gctcgctcct ggtctgctcc
      361 ggggtcgaag ggatcagcta ttcgaaggat cccaagaccg gctggtacac cccgaccccg
      421 gaggtcttca acgggtatcg cacgtgggac gggagcatcc tccgcaagac cggagtcggc
      481 gggtggacca acgtgctgcc caacatcgcc ggcttgaacg agcagggcca cgcctgggac
      541 atcaacgccg agtacggctt cctccaggac aagtgggtca tctacaacaa cgcggactgg
      601 aagtccttcg cgttcaactg gcccatcacg ccgtacagcg agctggacag cgacacccag
      661 gcggccgccc tggatgccag ctccaagatc ggcgcctacg cggatgaccg cctcgtcaat
      721 atcgcgctct ccaagagccc ggacgttctg aagtccgagt atgcgaagtt cgtctcccag
      781 atgaagaccg acggcctgga cgctttcgag gccgcctgga ccgacaactg gaaggcctac
      841 gccaagagca aaggaagggc gcccgagcac ttctttgtca cggtagacga ggccaagtag
      901 gatatcgagg caagcacggt gtccggcgga gtagaggaag cacgcggccg caggccgggc
      961 cgcgggagac ggtttctctc tcagtggcgg ttccagctcg tcctggtccc ggtgacgatc
     1021 tgggcgctcg tcttcttcta cctgccggcc gccggcaaca tcatcgcctt ccaggactat
     1081 cgactggaca aggggtacct gcgcagcagc ttcgtggggc tgaagcactt cgccgatttt
     1141 ctccagaacc agctcaccct ggtgctgctg cggaacaccc tggccatgag ctttctgggg
     1201 ctgatctcgg gcacggtcgc ggccatcgtt ttcgccatcc tgctgaatga gatcgtccgc
     1261 tcggcgccca agcgggtggt tcagaccgtc tcctatctcc cctacttcgt ctccatggcc
     1321 atctgcgcca atcttttcat ccagctcctg tcccggacgg gtcccatcaa ccagatgctc
     1381 ctgtccgcgg gcctctcgga caagccgatc cccttcctgg agatcgggcc cctgttctgg
     1441 tggatcctca ccctgcaggg cgtgtggaag aacgtggggt ggaacgcgat catctacatc
     1501 gccgccatca cgagcatcga cccccagatc ttcgaggcgg cctacgcgga cggagccggc
     1561 cggacccgaa ggatctggca catcacgctc ccgctgctcc tgcccaccgt tgccatcctg
     1621 ctcatcctca actcgggata cctgatcatg ggcgggttcg agcagcagct cctcatgttc
     1681 aaccccgtgg tcatggacta tgcggaggtc ctccagacct acgtctacaa gcgggggatc
     1741 ggtcaccagc agtattcttt tgccgcggcg gtgaacctgt tccagtccgt ggtgagcgtc
     1801 accatcatcc tggtcgtgaa ccggctgttc aagcggttca cggaaagcag catctggtag
     1861 gcagcggatg aaccaggctc aggcgtccgc tacgatcggt gagaaggcct tccgggtgtt
     1921 tctgttcgtg ttcttcctgc tgttcagcgt caccgtcgtg taccccttcg tctatctgct
     1981 ggccctgtcc ttcaacgaag ggacggacgc cttgaggggc gggatcacca tcttgccgcg
     2041 gatgttcacc gtggagaact accggatcat cttcgagaat ccccagctac tccacggcgt
     2101 gctcaactcg ttccttcgga ccgtgatcgg cgcctgctcg gcgacctttt tctcggggtt
     2161 cctgggctac acctttacca agcgcaagct ggtaggatat cggttctaca tcacgatcct
     2221 ggtcatcccc atgtacttca cggccggcat catcccgacg ttcctggtct atcgctccct
     2281 cgggctgatc aacaatttcc tggtgtacat cgtccccaac ctggtctggg cgtacaacat
     2341 catcatcatg cggaccttca tctccagcct gccccaggcc ctggaggagt cggcgttcat
     2401 cgacggagcc aacgagtaca ccatcttctt ccgcatcatc ctgcccctgt ccctgcccgc
     2461 gttcgccacc atcgtgctct tcgatgcggt ctggcactgg aatagctggt tcgacacgat
     2521 cctctacgcc cctcgggagg ggctggagac gctgatgagc atcctggcaa gaatgcttct
     2581 ggagctgcgg gcgcgggaaa tcgccgggct aaaggcctcc aagcatgcga tgaacatcac
     2641 ctcggagacg cttcgagccg ccatgaccat cgcgacgacg ctccccatcg tccttgtgta
     2701 cccgttcctt cagaagtact tcgtgaaggg cgtgttgatc ggggccgtca agggttgacg
     2761 gccggcgcgt gacgcgccgg gttcagggcg tggtcttggt caggtccacc gccagggaga
     2821 gatggttgat gccggcggcc ttcacgatct gaaggacctg caccaccacg tcgtacttta
     2881 cgtccttgtc tcccaggatc accaccggga catcggtcct ccccttgatc atggcggtga
     2941 tctggttgcg gaggtccgtg agggccacct tcgcctggtt cagatagacg ttgcccaccg
     3001 tgtcgatgct gatggtgacc gtctgaggct gctgggtgtc cgcgctggtg accttgggca
     3061 ggttcaccgt gatgccggtg cggaccaggg cgggcgtcgc gatcatgaag atgatgagca
     3121 acaccaggac cacgtcggtg aacggggtga tgttgatgcc cgagatgggg cggtcccggg
     3181 cggccctgga ctgccgcagt ctaccgcgca acgaggtccg ccacctctga ggcgcacagc
     3241 tccatgtccg tgacgaacgc ctcgatcctt ctggtgaagt agttgtagaa gatgaccgcg
     3301 ggcaccgcca cggccaggcc ggtagccgtg gtgatcagcg cctccgccac gcccccaatg
     3361 accacgttca tgctccccat ccccgcggcg gagatgtcct ggaaggcgcg gatgacgccc
     3421 aggaccgtgc cgaagaggcc gatatagacc gccgtgtttc cgatcgtgcc gaccaccccg
     3481 gtgaggcgct ccagcttcac ctcctccaac gtggtctgcc gctccatgac gccggccacg
     3541 aagcgctccg tgtggcccgc gcgctggaga cccgcgaggg ccaccttggc gaacggcgcc
     3601 tcgcaatcgt tgcacagctc gatggccttc ttgatgtcct tgcgtcccag ctcttcgcga
     3661 atgctctgca tgaacacggc ccgcttcacc cgggccttgg ccccgtagtt gaggagccgc
     3721 tcgatgatca ccgcgatgga cagcaccgag cagaagagca gcacgtacat cgtggggccg
     3781 cccatggcca ggatgtcggc gaatgtcttg ccagcaaaca tgaatccccc ttaacggagc
     3841 actatagcat caacgggcgg gagcgtccca cacgcgtttg aggccctccg ccaccctcgc
     3901 catcgccttg tccggcggca tctgggccag ctccgggacg aaaggctccg gcaccaccgg
     3961 accatcgtag ccgatcttcc gaagggccga cagaaagccg ggcaggtcga tggccacgcc
     4021 ctcccccgcg atcctgcgga cctggtcctg ctgcttctct actggaacac cgggcgcatc
     4081 gttgatgtgc acgtagacta cctggcgatt ctcgagctcc aggagctggt ccagggtgct
     4141 gaccgacgta taccagtggt acgcgtccaa cagcagcccc acgttcttcc cgacggcgtc
     4201 ccccagctcc agcatctgtt ctatagtatg ggcgaacgtg tagcggtggc cctcccgcag
     4261 ggaccgggga cccaggaact cgagggccag gcggcaccca tggtcttgaa ggatccgcgc
     4321 ggcctgaccg agttgggccg cgtgaagctt gaagttctcc cgatagggca gggtgtccga
     4381 ccaggagagg atccacgtag cgaaccgtgt cacgcctgcc gcctgggccc gagcggcgat
     4441 ggccggcagc ttcgagatct gggcctcgaa agccgtacgg tcctcactga ccctgacggg
     4501 gaatcccgtt ccacccggct tcagatggaa cttggaaagc ccctcccggt agcgttcgcc
     4561 cggaatgcct tcttccagcg gcacatcgat cccttcaaac ccgctatccc gggccagcgg
     4621 aaggcacgcc tcccaggaca ggtcgatccc aatcgctcca gggctgacac atcggtacac
     4681 ggcggttcct cctcgcgtga tcatcttacc actggcgttg gcccgtggct attctcggat
     4741 gtcacccggt
//
LOCUS       DASRZS010000188         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5103082, whole genome shotgun sequence.
ACCESSION   DASRZS010000188 DASRZS010000000
VERSION     DASRZS010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5103082"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..882
                     /locus_tag="VFH83_04635"
     CDS             31..882
                     /locus_tag="VFH83_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485682.1"
                     /translation="MRSLHAAETRVNVLLLLFFLVLCVAFLFPIYSLLLASFRPERDL
                     MRFGITVKTLAPGDLSLKYLASLLTARAGAYTVWYRNSLILVVMQTGLSLFFSSLVGY
                     GLAVYEFKGRRLLFSLVLLVLVVPIQILILPLYSLLIFFKSMNTFWGIVAPFVVSPFA
                     VFFFRQYAIGIPTDFIDAGRIDGLREGGIFFRVIAPLMKPAFGAMAILTALQSWNNFL
                     WPLIVLRTGDMFTLPIGLASLLSPYGNNYDMLISGSLMATLPILIVFFIFQRYFIAGL
                     TTGGIKG"
     gene            893..>2113
                     /locus_tag="VFH83_04640"
     CDS             893..>2113
                     /locus_tag="VFH83_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010898022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HET6485683.1"
                     /translation="MLSARIRLDREFVRSTIDDRLFGSFIEHLGRAVYGGIYEPGHPT
                     ADPDGFRGDVLGLVRELAVPIVRYPGGNYVSAFNWEDSVGPVRDRPRRLDLAWRSVEP
                     NTVGLDEFARWARRAGSEVMVALNLGTRGIDAARNLVEYCNHPGGSRWSDLRRAHGTA
                     EPHGFKVWCLGNEMDGPWQVGHKTADEYGRLAWEVAKALKLFDPTLQLVACGSSHSRM
                     PTFPDWEATVLDHCYESVDFISLHSYFQNHEDDLPTFLGESVEMDRFIRTVIGTVDFV
                     KARKRSRRTVNLSFDEWNVWFHSEKADKSVAPWTVGPPLLEDIYTFEDALVVGCCLIT
                     LLRHAERVRIACLAQLVNVIAPIMTETGGPSWRQTIFYPFLHASTFGRGEVLETLVKS
                     PVYENRKHGEVPYLE"
BASE COUNT          318 a          727 c          663 g          405 t
ORIGIN      
        1 cctgttcggg ttcttccgga gggaggaggc atgaggtccc tgcacgccgc cgaaaccagg
       61 gtgaacgtcc tgctcctcct cttcttcctc gtgctctgcg tggccttcct cttccccatc
      121 tattcgctgc tgctggcgag cttccgcccc gagcgcgacc tgatgcgctt cgggatcacg
      181 gtcaagacgc tggcgccggg agacctctcg ctcaagtacc tcgcgagcct gctcacggcc
      241 cgggccggcg cctacaccgt gtggtaccgc aacagcctca tcctggtcgt catgcagacc
      301 gggctgagcc tgttcttctc ctccctggtg ggctacggcc tcgccgtcta cgagttcaag
      361 ggccggcggc tcctgttcag cctggtcctg ctggtgctgg tggtccccat ccagatcctg
      421 attctcccgc tgtacagcct gctgatcttc ttcaagtcca tgaacacctt ctggggaatc
      481 gtcgccccgt tcgtggtttc cccgtttgcc gtgttcttct tccggcagta cgccatcggc
      541 attcccacgg atttcatcga cgcgggacgg atcgacggcc tgcgggaggg cggcatcttc
      601 ttccgcgtga tcgcgccgct catgaagccc gcgttcggcg ccatggccat cctcacggcc
      661 ctgcagagct ggaacaactt cctgtggccg ctgatcgtgc tgcgcacggg tgacatgttc
      721 accctgccca tagggctcgc cagcctcctc tcgccctacg gcaacaacta cgacatgctc
      781 atctcgggct cgctcatggc gaccctcccg atcctcatcg tcttctttat cttccagcgc
      841 tacttcattg ccggcctcac cacgggcggc atcaagggat aggagacctc gcatgctgag
      901 cgcccgtatc cggctcgacc gggagttcgt ccgttccacg atagatgacc gcctgttcgg
      961 ctcgttcatc gagcatctcg ggcgggcggt ctacggcggc atctacgagc ccggccaccc
     1021 caccgcggac ccggacggat tccgcggaga cgtgctcggg ctggtccggg agctcgcggt
     1081 gcccatcgta cgctaccccg gcggcaacta cgtctcggcg ttcaactggg aagacagcgt
     1141 ggggcccgtg cgcgaccggc cgcgccgcct ggacctggcg tggcggagcg tggagcccaa
     1201 caccgtgggg ctggacgagt tcgcccgctg ggcgcgccgg gccggctcgg aggtgatggt
     1261 ggccctgaac ctgggcacgc gcgggatcga cgccgcgcgc aacctcgtgg agtactgcaa
     1321 ccaccccggc ggctctcggt ggagtgacct tcggcgggcc cacggtacgg cggagcccca
     1381 cggattcaag gtctggtgcc tgggaaacga gatggatggc ccctggcagg tgggccacaa
     1441 gaccgcggac gagtacgggc gcctggcctg ggaagtggcc aaggcgctga agctcttcga
     1501 tccgacgctc cagctcgtgg catgcgggag ctcccactcg cggatgccga ccttcccgga
     1561 ctgggaagcc acggtcctgg atcactgcta cgagtcggtg gacttcatct cgctgcacag
     1621 ttacttccag aaccacgagg acgacctgcc gacgttcctc ggcgagtcgg tggagatgga
     1681 ccgcttcatc cggacggtga tcggcacggt ggacttcgtg aaggcccgaa agcgcagccg
     1741 gcgcacggtg aacctctcct tcgacgagtg gaacgtgtgg ttccattcgg aaaaggccga
     1801 caagtcggtg gcgccctgga ccgtcggccc cccgctcctt gaagacatct acacgttcga
     1861 ggatgccctg gtggtaggct gctgcctgat caccctgctg cgccatgccg agcgcgtgcg
     1921 gatcgcctgc ctcgcccagc tcgtcaacgt gattgcgccg atcatgaccg agaccggcgg
     1981 tccctcctgg cggcagacca tcttctaccc cttcctgcac gcgtccacgt tcggccgggg
     2041 agaagtgctg gagaccctgg tgaagtcccc ggtgtacgag aaccgcaagc acggagaggt
     2101 tccgtacctc gag
//
LOCUS       DASRZS010000189         5449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3948540, whole genome shotgun sequence.
ACCESSION   DASRZS010000189 DASRZS010000000
VERSION     DASRZS010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3948540"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VFH83_04645"
     CDS             <1..526
                     /locus_tag="VFH83_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485684.1"
                     /translation="LWFFIFSPTYGLANGFLAWFGIPAQQWLWDVHLVKPSLVIMAVW
                     SFGQAMIIFLAGLQGVPLALYEAADIDGASAWRRFRSITLPMLSPVIFFNLVIGIIGS
                     FQVFTSVLVMTNGEPANASLMVVLYIYRNAFQNFKMGYASLLSWVLLVIVLILTLIQF
                     RLSRGWVYYEGERQ"
     gene            523..1389
                     /locus_tag="VFH83_04650"
     CDS             523..1389
                     /locus_tag="VFH83_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485685.1"
                     /translation="MTSAASFFSSRSGKRVVAHGATYLVLVAIAFVSLIPLLYMVSTS
                     LKPNGTEYEFPIRWIPARIAWENYPKAFTSVSTLVFLKNTLIITAVSLIGELLTASLV
                     AYGFARLRFPGRNVLFALMLSTLMLPYIVVMIPLFVLFRYLHWINTLLPLTVPAFFGG
                     GPLYIFLLRQFFMTLPTELDDAAKIDGAGFFRVWWTILMPLARPALVTVAILSIVFHW
                     NDFAAPLIYINTNDKMTLSLGIRLFRDQYTTHFNLTMAFSTMMTLPIITVFFLFQKYF
                     IQGISTTGLTGR"
     gene            1535..2815
                     /locus_tag="VFH83_04655"
     CDS             1535..2815
                     /locus_tag="VFH83_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET6485686.1"
                     /translation="MKAYRLALCAILILAVASLAGAAAKTKIRATVWLGDAELKALGS
                     MTDAYLKMHPNADVEWINIVGGGNYGRDKLQTMIAGGDAPDLMMLNTGQFEQLASRGA
                     LKPLDDIIKGEKFDLSLYWPQAIGGISYQGKIYGLPRDMSNVILYYNKDLFDKSGVAY
                     PNNNWTWNDLLAAAKKLTLDTNHDGKIDQWGFAVNNIVWVWAGFVWGNGGDILNADRT
                     KVLLNDPNTIDALNFYFGLLTKEHVSPPPGALPEQSWAGDWMLTQNVAMGLFGPWWRP
                     SLVTMDKPFRWDVAYPPKSPKTGKRGSVVYTDMWGMSSSTGIQAQTWDFMKYLTSKDG
                     QQLWTDLIGARSISPVKSVAQSDKWLHYGGSTGEIILDSLSFSQVPPINFGNGNEVET
                     IWDQEFGAVIAGQQTVEQAVAKIIPQVQAVLSGN"
     gene            complement(2894..3778)
                     /locus_tag="VFH83_04660"
     CDS             complement(2894..3778)
                     /locus_tag="VFH83_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6485687.1"
                     /translation="MPTVSIRGAHIHYQIHGTGVPVAFTPGGFWGLDDAVPFAEALSL
                     LGCRVVIHDRPNCGASDIVLDDSRTVYDLFADYLHGLLAELRLLPCVIAGASAGAVTS
                     LAFARRHPESVRGVVLISPPTDDPGAWQAIARSTFQQPARLAELGGMAAVAEISLGFF
                     DFPSRIAANPRNRDALLSMDPKRFAAVLRRWGESAASGREHVAGLSDAEIAAIAAPAL
                     VICGPDPRNGLHPRHTAERLHELIAGSRLIVPYEALPTEVVEKVRAQVAEGGGRLYDA
                     ALAPFVRDFALSLGAAPA"
     gene            3933..4964
                     /locus_tag="VFH83_04665"
     CDS             3933..4964
                     /locus_tag="VFH83_04665"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET6485688.1"
                     /translation="MSPAPIDWYRVPLSREQLRSYTEKSSLRGWIQACSVLLLFAATS
                     TSAFLLFRARLWLPMVIVCYFHSFFLHTIGIGGGIHELSHGTVFRGKRTNEFFFHLYC
                     FLAWNNPVHFRASHLLHHQYTLYAGQDKEVVQVPVAQKLGLLSWVSAFTFDYRSFAHM
                     VRVNVLHAVGNANPDFYFWDPLFPEGDPRRKALCRWALFMVLAYVALTVVLAALGLWV
                     LIYVLVLGSFFGTALARVVTVLQHSGLASSVPDWRLVCHTMDLNPVFRFFYWQMSFHT
                     EHHMYAAVPFHALPRFSREAAGRMQAHIPGIRAGVALLRRIRSDQENDPGYVYSPRFP
                     VTADPPRRV"
     gene            4984..>5449
                     /locus_tag="VFH83_04670"
     CDS             4984..>5449
                     /locus_tag="VFH83_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6485689.1"
                     /translation="MVRPSVADWFAGNVLEYVDGYRNRPDASFPFHDHPCHEIVYHPQ
                     GRGKTTLQDGREIPFEQGGVVFYKPFDRHSQTVSTPALDVVIQFTGKNLPPPMQVSRG
                     FRPFPQHWVNDTMMELSRRAHKLPREERLLLNHRVSTLVMALFTLGESYEQTR"
BASE COUNT          928 a         1825 c         1665 g         1031 t
ORIGIN      
        1 cctctggttc ttcatcttca gtccgaccta cggtcttgcc aacggcttcc ttgcctggtt
       61 cgggatcccg gcgcagcagt ggctgtggga cgtgcacctg gtgaagccgt ccctggtgat
      121 catggcggtc tggtccttcg gccaggccat gatcatcttc ctggcgggtc tgcagggcgt
      181 gccgctggcc ctctacgagg cggccgacat cgacggggcc agcgcgtggc ggcggttccg
      241 ctccatcacg cttcccatgc tctctccggt gatcttcttc aacctcgtga tcggaatcat
      301 cggctcgttc caggtgttca ccagcgtgct ggtcatgacc aacggcgagc cggccaacgc
      361 ttccctgatg gtcgtgctct acatctaccg aaacgccttc cagaacttca agatgggata
      421 cgcctcgctg ttgtcgtggg tgctgctggt gatcgtcctg atcctcacgc tgatccagtt
      481 ccggctctct cgcggctggg tctactacga gggggagagg cagtgacctc cgcggcgagc
      541 ttcttctcca gcaggtccgg gaagcgggtc gttgcccacg gtgcgacgta cctggtgctg
      601 gtggccatcg ccttcgtctc cctgatcccc ctgctctaca tggtcagcac gtcgctcaag
      661 cccaacggca cggagtacga gttccccatc cgatggatcc cggcccggat cgcctgggag
      721 aactatccga aggccttcac ctcggtctca acgctggtct ttctcaagaa caccctgatc
      781 atcaccgcgg tatccctgat cggggagctc ttgaccgcgt ctctcgtggc gtacggcttc
      841 gcccggctgc ggttccccgg ccgaaacgtg ctcttcgcgc tcatgctgag caccctgatg
      901 ctgccgtaca tcgtcgtgat gattccgctg ttcgtcttgt tccgatatct gcactggatc
      961 aacacgctcc tgccccttac cgtgccggcc ttctttgggg gaggccctct gtatatcttc
     1021 ctgctccgcc agttcttcat gacgctcccg acggagctgg atgacgccgc caagatcgac
     1081 ggcgccggct tcttccgcgt ctggtggacc atcctgatgc ctctggcgcg gcccgcgctg
     1141 gtcaccgtcg cgattctctc catcgtcttc cactggaacg acttcgccgc gcccctgatc
     1201 tacatcaaca cgaatgacaa gatgacgctc tccctgggga tccggctgtt tcgggaccag
     1261 tacaccactc atttcaacct gaccatggcc ttctccacca tgatgacgct gccgatcatc
     1321 acggtgtttt tccttttcca gaagtacttc atccagggga tatccacgac cggcctgacg
     1381 ggccgatgac ccattcgccc ggcggctcct cggaccggag cccggaaagg aggattggac
     1441 aggaccggcg gagggaggcc gccgagggca agctcttacg cggaggcggg tctcgatgca
     1501 agcccggctt cgctgaagga tgaggaggtt ccctatgaaa gcgtatcggt tggccttgtg
     1561 cgccatcctg atcctggcgg tggcatccct cgcgggcgcc gctgcaaaga ccaagatccg
     1621 cgcaacggta tggctcgggg acgccgagct caaggctctg ggctccatga cggacgccta
     1681 cctgaagatg catcccaacg cggatgtgga gtggatcaac atcgtcggcg gcggcaacta
     1741 tggccgcgac aagctgcaga ccatgatcgc cggaggtgac gcgccggacc tcatgatgct
     1801 gaacaccggg cagttcgagc agctggcctc ccgaggcgcc ctgaagcctc tggacgacat
     1861 catcaagggg gagaagttcg atctcagcct ctactggccc caggccatcg gcgggatcag
     1921 ctaccagggc aagatctacg gcctgccgag ggacatgagc aacgtcatcc tgtattacaa
     1981 caaggacctg ttcgacaagt ccggtgtcgc ctatcccaac aacaactgga catggaacga
     2041 tctgctggcg gcggcgaaga agctcacgct cgacacgaac cacgacggca agatcgacca
     2101 gtggggcttc gcggtgaaca acattgtctg ggtgtgggcg ggattcgtgt ggggcaacgg
     2161 cggtgacatc ctcaacgccg accggaccaa ggtcctcctg aacgatccca ataccatcga
     2221 cgccctgaac ttctacttcg ggctcctgac caaggagcac gtctcgccac ctcctggggc
     2281 actcccggag cagagctggg caggggactg gatgctcacc cagaacgtgg ccatggggct
     2341 gtttggcccc tggtggcgcc ccagcctggt gaccatggac aagccgttcc ggtgggacgt
     2401 ggcctatccg cccaagtcac ccaagacggg caagcgcggc tccgtcgttt acacggacat
     2461 gtgggggatg tcttccagca cggggatcca ggcccagacc tgggacttca tgaagtacct
     2521 gaccagcaag gacggacagc agctctggac ggacctgatc ggggcccggt ccatctctcc
     2581 cgtgaagtcc gttgcgcaga gcgacaagtg gctgcactac ggcggctcga ccggtgagat
     2641 catcctggat tctctgtcct tctcccaggt gccgcccatc aacttcggga acggcaacga
     2701 agtggagacc atctgggacc aggagttcgg cgcggtgatc gccgggcagc aaaccgtgga
     2761 gcaggcggtg gccaagatca tccctcaggt acaggcggtc ctgtccggga actgatgtcc
     2821 gaaagttggg tagtgtccag gggggcggtt ccgtccgtcc ccctgcgttt ccgcctcgga
     2881 tggccgcttc agctcaggcc ggtgcggccc caagactcag cgcaaagtct ctgacaaagg
     2941 gcgccaacgc cgcgtcatac agccttccac ccccctcggc gacctgggcc cgtaccttct
     3001 ccacaacctc cgtcggcagg gcctcgtacg ggacgatgag tctggacccc gcaatgagct
     3061 cgtgcagccg ctcagcggtg tgacgagggt ggagcccgtt ccgcggatcc ggaccgcaga
     3121 tcacgagcgc cggcgctgcg atggctgcaa tctcggcatc cgaaagcccc gctacgtgct
     3181 ccctgccgct cgccgctgat tcgccccagc gcctcagaac cgctgcgaac cgcttcgggt
     3241 ccatcgagag aagggcgtct cggttcctgg ggttcgccgc gatccgactc ggaaagtcga
     3301 agaagccgag ggagatctcg gcaacggcgg ccatgccgcc aagctccgcc agccgcgccg
     3361 gctgctggaa ggtcgagcgc gctattgcct gccaagcgcc ggggtcatcc gtcggcgggc
     3421 tgatgaggac gacgccccga acggactcgg ggtgtctgcg agcgaaggcc agcgaggtga
     3481 cggcaccggc cgacgcccca gcgatcacgc agggcagcag ccgaagctcg gcgagcaggc
     3541 catggaggta gtccgcgaag aggtcgtaca cggtgcggga atcgtccagc acgatatccg
     3601 atgcgccgca gttgggcctg tcgtggatta cgacccggca gccgaggagg ctcagggcct
     3661 cggcaaaggg aaccgcatcg tccaaccccc agaatccgcc gggagtgaac gccaccggca
     3721 cgccggtccc atggatctgg tagtggatgt gggcgccacg tatggagacg gtgggcatag
     3781 gaacctcctg cgcggcacgg gccccgcctt ccgcgccaac cctctcaccc ggtcaccgtg
     3841 acgtcaagcg tccgcagagg tcagcggatc ccgcgggtcg gccgaacgcc gacttgacac
     3901 ggctcactcc tcgtcagagg ctcccgggta ccatgtcccc agcaccgatc gactggtacc
     3961 gcgttcccct gtccagggag cagctccgct cctacacgga gaagagcagc ctccgcggct
     4021 ggatccaggc ctgcagcgtg ctcctgctgt tcgccgccac gtccacctct gcctttctgc
     4081 tgttccgagc gcgcctgtgg ctccccatgg tgatcgtctg ctacttccac tccttcttcc
     4141 tgcacaccat cgggatcggc ggtgggatcc atgagctcag ccatggcacg gtgttccgag
     4201 gcaagaggac gaacgagttc tttttccacc tttactgctt cctggcctgg aacaacccgg
     4261 ttcacttccg agccagccat ctcctgcacc accagtacac gctgtacgca ggccaggaca
     4321 aggaggtggt ccaggttccc gtggcccaga agctcggcct tctgagctgg gtgagcgcat
     4381 tcaccttcga ctaccggagc tttgcccaca tggtccgggt caacgtcctg cacgcggtcg
     4441 gaaacgcgaa tccggacttc tacttctggg acccgctgtt ccccgagggc gatccgcggc
     4501 gcaaggccct gtgtcgatgg gcgcttttca tggtccttgc ctacgtggcg ctgaccgtgg
     4561 ttctggccgc gctcggcctc tgggtcctga tctacgtgct ggtcctgggg agttttttcg
     4621 gcaccgcgct ggcccgggtg gtgaccgtcc tgcaacattc cgggctggct tcgagcgtcc
     4681 cggactggcg cctggtctgc cacacgatgg acctcaaccc cgtgttccgc ttcttctact
     4741 ggcagatgag cttccatacg gagcaccaca tgtacgccgc cgtgccgttt cacgcgcttc
     4801 cccggttcag tcgggaagcc gccggccgga tgcaggccca tatccccggg atcagggcag
     4861 gcgtggccct gctgaggcgc atccgctcgg accaggagaa cgatccgggc tacgtctact
     4921 ctccgcgctt cccggtcacc gcggatcctc cccgtcgagt ctgaggggtt cgaggagacg
     4981 cgcatggttc gtccgtccgt tgcggactgg ttcgccggca acgttttgga atacgtggac
     5041 ggataccgca accgccccga cgcatcgttt cccttccacg atcatccctg ccacgagatc
     5101 gtgtatcacc cccagggccg cgggaagacg acgctccagg acggccgtga gatcccgttc
     5161 gagcagggtg gggtggtgtt ctacaagccc tttgaccgcc actcccagac cgtcagcacg
     5221 ccggcgctgg atgtcgtaat ccagttcacg ggcaagaacc tgccgccgcc aatgcaggtg
     5281 agccggggct tccggccctt cccccagcac tgggtgaacg acacgatgat ggagttgtcc
     5341 cgccgcgccc acaaactgcc ccgcgaggaa cggctgctgc tgaaccatcg cgtgtccact
     5401 ctggtcatgg ccctgttcac cctcggagag tcctacgaac agacccggg
//
LOCUS       DASRZS010000190         2774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5491208, whole genome shotgun sequence.
ACCESSION   DASRZS010000190 DASRZS010000000
VERSION     DASRZS010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5491208"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VFH83_04675"
     CDS             complement(<1..688)
                     /locus_tag="VFH83_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET6485690.1"
                     /translation="MNYYEILGVPQNSTTDEIKRSFRRRAKELHPDVRPGEGGEEGMR
                     ELLAAYEVLSDIEKRLEYDRALRTAFRSRGFDYREFLRGRPDDPVSQSKLIFHDLLTN
                     HQDDAIELYHRLTTERGFQLERYLNREDYMDCAFLLAEVFEGHDRLREAYELYRRVFY
                     FEQEKPYFRHFTEEVIDRLRILLCSKMLPGLEPLEAVGRLKEMIRLDFSRKDNAFFYK
                     KLAEVYSSLGQ"
     gene            845..1618
                     /locus_tag="VFH83_04680"
     CDS             845..1618
                     /locus_tag="VFH83_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485691.1"
                     /translation="MFGFLVKKAFFDLWDNLFRILIMNLGFIAVGAIFFLLAPLLTPV
                     PVLFFATIAIGIGVLAVYTGAASRMCSEIADYRQPGFSDFYRYVQETFPSSLLLSLVI
                     LAYVFIISVAFGFYGGLKSLAGPLAIALLFWVTVAGVFAVQYFFPIQSRMDRKFRKIA
                     RKCFLLFFDNPGFSIGLLFGALVTLVISVFTALLLPGITAVLLWWNVAFKLRLYKYDW
                     LEENPGANRKRVPWDALLIEDRERVGKRTIRGMIFPWKE"
     gene            complement(1664..2077)
                     /locus_tag="VFH83_04685"
     CDS             complement(1664..2077)
                     /locus_tag="VFH83_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485692.1"
                     /translation="MRVAVVFFPGRSREKLMSLAKALGRGIEKQGNHVDVFDGVQDSN
                     TKLTIYQYLVVATEAVGTFGGKIPDTVKTFLSGAGAVAGKKSYAFIPKATFGAAKSLA
                     ALMRCMEGEGMVLKNSDIVRSAVEAEEIGRRLHVS"
     gene            complement(2037..>2774)
                     /locus_tag="VFH83_04690"
     CDS             complement(2037..>2774)
                     /locus_tag="VFH83_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485693.1"
                     /translation="TESHLATLRHRYDERRLREAATRRAHLDYQRIPIGGAAGSGRVK
                     FLLAQMHQTYYFGFSESACILAGTVLEQGLVCRLGDCLTTRGPLSVTRSGARRWLQNR
                     DDLLELELVDMMDLARAEGILRDGRTMLLAHHIRWIRNMVVHERIPLFRARDEEHLEL
                     VVTKSRRGRPRTTRISLDRSEVRELEALSDAAGEERGSRGLAAQLTAYYCVSRTRMVL
                     RSLFTQPHTETRKDDESSGSLFPWAQS"
BASE COUNT          453 a          911 c          842 g          568 t
ORIGIN      
        1 tctgccccag cgacgaatag acctcggcga gcttcttgta gaagaacgcg ttgtccttgc
       61 gggagaaatc gaggcggatc atctctttca gtctcccgac ggcctccagc ggctcgagac
      121 ccggcagcat cttcgagcac aggaggatcc ggagccggtc gatgacctcc tcggtgaagt
      181 gacggaagta cggcttctcc tgctcgaagt agaagacccg gcggtacagc tcgtatgcct
      241 cccgaaggcg gtcgtggcct tcgaagacct ccgccaggag gaaggcgcag tccatgtagt
      301 cttcccgatt caggtatcgc tccagttgga agcctcgctc ggtcgtcagc cgatggtaga
      361 gctctatcgc atcgtcctgg tggttggtca gaaggtcatg gaagatgagc ttggactggc
      421 tgaccgggtc gtccggcctg ccgcgcagga actcccgata gtcgaacccg cgcgagcgaa
      481 acgcggtgcg gagcgcccgg tcgtactcca ggcgcttctc gatgtcgctc aggacctcgt
      541 aggcggcgag gagctcgcgc atcccctcct caccaccctc accgggccgc acgtccgggt
      601 gcagctcctt ggccctgcga cgaaacgacc gtttgatctc gtccgtggtg ctgttctgcg
      661 gtacacccag gatctcgtag tagttcatag ggaccaaagg gtttctgcca aaaggtctcc
      721 gaatgatagt atccggccga gggcggtgtc aagacttcgg accgttggga tggaccggtt
      781 cggcccaaaa aactccaggt gccgcgcatt tgacctgcaa gacattcggt gctatggtcg
      841 aaccatgttc ggcttcctcg tcaagaaggc tttcttcgat ctatgggaca acctgttccg
      901 gatcctgatc atgaacctgg ggttcatcgc ggtcggcgcg atcttcttcc tgctcgcgcc
      961 cttgctgacc ccggtgcctg tcctgttctt cgcgacgatc gcgatcggga tcggggtcct
     1021 cgccgtgtac acgggggcgg caagccgtat gtgctcggag atagcggact atcgccagcc
     1081 gggcttctcc gacttctacc gctacgtcca ggaaaccttc ccttccagtc tccttctttc
     1141 gctggtcata ctcgcgtacg tcttcatcat ttccgtagcg ttcggattct acggcggtct
     1201 gaagagcctg gccggcccgc ttgccatcgc cctcctcttc tgggtcaccg tggcaggggt
     1261 cttcgcggtt cagtacttct ttccgatcca gtcccgcatg gaccgcaagt tccgaaagat
     1321 cgcgcggaag tgcttccttc tctttttcga caaccccggg ttttcgatcg ggctcctgtt
     1381 cggcgccctg gtcaccctcg tgatctccgt gttcacggcg ctgcttctac ccggcatcac
     1441 ggcggtcctg ttatggtgga acgtcgcctt caagctgcgg ttgtacaagt acgactggct
     1501 ggaagagaac cccggtgcca accgcaagcg ggtcccctgg gacgccctgc tcatcgagga
     1561 tcgggaacgg gtcgggaaac gtacgatccg cggcatgatc ttcccctgga aggagtgacc
     1621 gccgccgggt ccgggcgcga ccgctcgggc ggcgcccgtc ccttcagctc acgtgcagcc
     1681 gccttccgat ctcctccgcc tccaccgcgg agcggacgat atccgagttc ttgagaacca
     1741 tcccctcccc ttccatgcat cgcatcaggg ccgcgagaga cttggcggca ccaaaggtgg
     1801 ccttgggaat gaaggcatag cttttcttgc cggcaacggc cccggccccg gacaggaagg
     1861 tcttcaccgt atccggtatc ttgcccccga acgtccccac ggcctccgtg gcgaccacca
     1921 ggtactggta gatcgtcagc ttcgtgttgg agtcctgaac gccgtcgaac acgtcgacgt
     1981 ggttgccctg tttctcgatg ccgcgcccca gggccttggc cagcgacatc agcttttcac
     2041 gactgcgccc agggaaaaag actaccgcta ctctcatcgt ccttcctcgt ctccgtgtgg
     2101 ggttgggtga acagactccg tagaaccatc cgcgtgcggc tcacgcagta ataggcggtg
     2161 agttgggcgg cgaggccccg ggaaccgcgc tcctccccgg cggcatcgga gagggcttcc
     2221 agctcccgca cctcggagcg gtccagactg atccgggttg tgcgcggacg ccctcgtcga
     2281 ctcttggtga ccaccagctc caggtgctcc tcgtcccggg ctcgaaacag cggtatccgc
     2341 tcgtggacca ccatgttccg gatccatcgg atgtgatgcg ccaggagcat ggtgcggccg
     2401 tctctcagga tcccctccgc gcgcgccagg tccatcatat cgaccagctc cagctccagc
     2461 aggtcgtcgc ggttctgcag ccagcgtcgc gccccggatc gggtgaccga gagagggccg
     2521 cgggtggtca ggcagtcccc gagccggcag accaggccct gctccaggac cgtgcccgcg
     2581 aggatgcagg cgctctccga aaacccgaag tagtaggtct ggtgcatctg ggccagcagg
     2641 aacttcaccc ggccgcttcc ggcggcccca ccgatgggaa tgcgctggta atcgaggtgc
     2701 gcgcgccggg ttgctgcctc gcgaaggcgc cgctcgtcgt atcggtgcct cagcgttgcc
     2761 agatggctct cggt
//
LOCUS       DASRZS010000191         3426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_103595, whole genome shotgun sequence.
ACCESSION   DASRZS010000191 DASRZS010000000
VERSION     DASRZS010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103595"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFH83_04695"
     CDS             complement(<1..581)
                     /locus_tag="VFH83_04695"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009332837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6485694.1"
                     /translation="MTYRTLGRSGIRVSTVGLGCMSLGLDRARGESIVHRALDLGVTL
                     FDTADLYDHGVNEELVGAALRGARHEVVIATKVGNRWRADGSGWDWDPSARHVLQGVH
                     DSLRRLGTDYIDLYQHHGGTIEDPFDETVEAFERLVREGLIRAWGISSIRPNVVRRYA
                     SRLAQGGAPHLTSEMVQYSLLDRRPEEEILGTAR"
     gene            complement(596..2101)
                     /locus_tag="VFH83_04700"
     CDS             complement(596..2101)
                     /locus_tag="VFH83_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6485695.1"
                     /translation="MAKRVVIIGAGIGGLAAGCYARMNGYDAQILESHTTPGGLCTSW
                     KRKGFTVDGSCHWVTGSGPASPYYTIWNEIGAIQDRTYIDYDYFVRFQDANGRVFSLY
                     TDADRLEAHMKELSPQDAAPTEELCRMIREFSRFSMPIRKPPELMNLMDGLLLMRKFG
                     PFRKLFALAGAFSAEGFSSRFKDPLLREAIHNALFGASPSLFPLIMTMGTMHARTAGY
                     PLGGSLEFARAIERRYLSLGGTVRYGARVSKVVERDGKAVGVELSGGETVEADWVISA
                     CDMKWTLQGLLDGTRMDPVHRELLQTGVTIPPAIQVSFGVDMDLSDRETGFNQGFRLN
                     QPLCIGGKTIEWYNYKAYSFDPSMAPKGKSTVVTIFLGDWKYWEKLKANPILYKEEKA
                     RIAQDCARAMDQRFPGFSSKVEMSDVATPLTFERYTGNWKGSYMTWILSPEFQRKHRY
                     VPKTVPGLDRFYMASMWTNPPGGLPGAASAGRGVIQLLCARDRRRFVTSTP"
     gene            complement(2188..>3426)
                     /locus_tag="VFH83_04705"
     CDS             complement(2188..>3426)
                     /locus_tag="VFH83_04705"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HET6485696.1"
                     /translation="VEPGPPVVLSHVRAVLVARDLEYVGSFRCDDERLNAIWRTGAYT
                     VQLTMQDYLWDGIKRDRLVWLGDMHPEVSTINAAFGACPVVPRSLDLIRDVTPGTRWM
                     NGISSYSMWWVLIQEDWWMHHGDRAYLEDQRGYVRELLRRLSGFVGKDGSETLDGHRF
                     LDWPTSGDPRAVHEGLQAMMVLTMDAGARLSRALREEETATLCSETAGRLRRHQPEPG
                     GRKSPAALAALAGLREAREVSDGVLKPGGPRDLSTFYGFYVLQALAKAGDIDTALSFV
                     STYWGGMLDLGATTFWEDFDLAWSRNAARIDDLVPSGKRDIHGECGDYCYKGFRHSLC
                     HGWASGPTAWLSQHVLGIRPQEPGFSSTLVRPQLGRLRWAEGSYPTPLGPIRVRHEKA
                     PDGSTRSEIAAPPGVRITQK"
BASE COUNT          552 a         1189 c         1105 g          580 t
ORIGIN      
        1 cgcgccgttc ccaggatctc ctcctccggc ctgcgatcca ggaggctgta ctgcaccatc
       61 tccgaggtga gatggggcgc cccgccttgc gccagccgag acgcatacct tcggaccacg
      121 ttcgggcgga tcgacgagat cccccacgcg cggatgagcc cctctcggac gagccgctcg
      181 aacgcctcca ccgtctcatc gaacgggtcc tcgatggtcc ccccgtgatg ctggtagaga
      241 tcgatgtagt cggtccccag ccgtcgcagg ctatcatgga cgccctggag gacatggcgg
      301 gccgacggat cccagtccca gcccgatccg tccgcgcgcc agcggttccc caccttggtg
      361 gcgatcacca cctcatggcg agctccgcgc agggcggctc ccaccagctc ctcgttgacc
      421 ccgtggtcgt agagatccgc cgtgtcgaag agcgtcacgc ccaggtcaag cgcccgatgg
      481 acgatgctct ccccccgggc cctgtcgagc cccagcgaca tgcatcccag tcccaccgtg
      541 gaaacccgga taccggaccg gcccagcgtt cggtacgtca tggtcgaggc tccccctagg
      601 gtgtgctcgt aacgaaccgc ctgcggtccc gagcgcagag aagctggatc accccgcgtc
      661 ccgcgcttgc ggcgccgggg aggcctccgg gcgggttggt ccacatgctg gccatgtaga
      721 accgatcaag gcccggcacg gtcttgggga catagcggtg cttcctctgg aactcggggc
      781 tcaggatcca ggtcatgtag gaccccttcc agttgccggt gtaccgctcg aaggtgaggg
      841 gggtcgccac gtcgctcatc tccaccttgg agctgaagcc ggggaaccgc tggtccatgg
      901 cccgggcgca gtcctgggcg atgcgcgcct tctcttcctt gtagaggatc gggttggcct
      961 tcagcttctc ccagtacttc cagtctccga ggaagatggt cactacggtg gacttcccct
     1021 tgggggccat ggacggatcg aacgagtagg ccttgtagtt gtaccactcg atggtcttgc
     1081 cgccgatgca cagcggctga ttcagtcgga atccctggtt gaatccggtc tctcgatcgg
     1141 agaggtccat gtccacgccg aagctcacct gaatggcggg cgggatggtg actcccgttt
     1201 gcaggagctc ccggtgcacg gggtccatgc gcgtgccatc cagcagaccc tggagggtcc
     1261 acttcatgtc gcacgccgag atgacccagt ccgcctcgac ggtttcgccg ccggacagct
     1321 cgacccccac cgccttcccg tcccgttcga ccaccttcga gacgcgtgcg ccgtagcgga
     1381 ccgtcccccc cagggaaagg taccgccgct cgatggcgcg ggcgaactcg agggatccgc
     1441 cgagggggta gccggccgtc ctggcgtgca tcgtccccat ggtcatgatg agcgggaaca
     1501 gcgacggaga ggcgccgaac agcgcgttgt ggatggcctc ccgcaggagg ggatccttga
     1561 accgtgatga gaacccttcg gcgctgaacg ccccggccag ggcgaagagc ttccgaaacg
     1621 gcccgaactt gcgcatgaga aggaggccgt ccatgagatt catgagctcg ggtggcttgc
     1681 ggatgggcat ggagaaccgg gagaactccc ggatcatgcg gcagagctct tccgtgggcg
     1741 cggcgtcctg gggcgagagc tccttcatgt gggcttccag ccggtcggcg tccgtgtaca
     1801 aggagaacac ccggccgttg gcgtcctgga agcgcacgaa gtagtcgtag tcgatgtagg
     1861 tccggtcctg gatggccccg atctcgttcc agattgtata gtagggggag gcgggtcccg
     1921 agcccgtcac ccagtggcag cttccgtcaa ccgtgaagcc ctttcgcttc catgaagtac
     1981 acagcccacc gggcgtggtg tgggattcca ggatctgcgc gtcgtaaccg ttcatcctcg
     2041 cgtagcaacc cgccgccagc ccgccaatac ccgctccgat gatcacgacc cgtttcgcca
     2101 tctcgacctc ctgacacccg tcggatcgtc gctcaggacc gccccggggt ccttacttca
     2161 acccggggca ccccgctttg tgtcctgcta cttttgagtg atcctgactc ccggcggcgc
     2221 ggcgatctcc gatcgggtcg acccgtccgg cgccttctcg tgccggaccc ggatcggccc
     2281 cagcggggta ggatagcttc cctccgccca ccgcagccga ccgagctgag gccggaccag
     2341 ggtcgaggag aaacccggct cctgcggtcg gatcccgagg acgtgctgac tgagccacgc
     2401 ggtcggaccc gacgcccagc cgtggcagag gctgtgccga aagcccttgt agcagtagtc
     2461 cccacactcg ccgtggatgt ccctcttgcc ggacggcacc aggtcgtcga tgcgggcggc
     2521 gttccgggac cacgcgaggt cgaagtcctc ccagaacgtc gtggctccga ggtccagcat
     2581 ccctccccag taggtggaga cgaagctcag cgccgtgtcg atatcccccg ccttggcgag
     2641 cgcctgcaga acgtagaaac cgtagaaggt cgagaggtcc cgcggaccgc ccggcttcag
     2701 gaccccgtct gacacctcgc gcgcttcgcg caaccccgcc agggcggcga gggctgccgg
     2761 ggacttccgg ccgcccggct ccggctgatg gcgccgcagg cggcctgccg tctccgagca
     2821 gagcgtagcg gtctcctcct cccggagcgc gcgggacagg cgggcaccgg cgtccatcgt
     2881 cagcaccatc atcgcctgca ggccttcgtg cacggcccgg gggtcgccgc tggttggcca
     2941 gtccaggaag cgatgaccgt ccagcgtctc ggagccatcc ttccccacga agcccgaaag
     3001 ccgtcgaagc agctcccgga cgtacccgcg ctgatcctcc agataggcgc ggtccccgtg
     3061 gtgcatccac cagtcctcct ggatgaggac ccaccacatc gagtaggagc tgatgccgtt
     3121 catccatcgg gtgcccggag tgacgtcgcg gatcaggtcc aggctccgcg gcacgaccgg
     3181 gcaggcgccg aacgcggcgt tgatggtgct gacctcgggg tgcatgtccc ccagccagac
     3241 caggcggtcg cgcttgatgc cgtcccagag gtagtcctgc atggtgagct ggacggtata
     3301 ggctccggtg cgccagatcg cgttcagccg ttcatcgtca cagcggaagg agccgacgta
     3361 ctccaggtcc cgggcaacca gcaccgccct cacgtggctg aggaccaccg gcggtcccgg
     3421 ttccac
//
LOCUS       DASRZS010000192         6836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4723789, whole genome shotgun sequence.
ACCESSION   DASRZS010000192 DASRZS010000000
VERSION     DASRZS010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4723789"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFH83_04710"
     CDS             complement(<1..291)
                     /locus_tag="VFH83_04710"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HET6485697.1"
                     /translation="MKKFINHPKRFVDDMLEGIYRAHPDQVTAAAGDPRCLVRVSSPR
                     KGKVGLATGGGSGHLPLFLGYVGEGMLDGCSVGGVFQSPSADQMLTVTRAISG"
     gene            792..1796
                     /locus_tag="VFH83_04715"
     CDS             792..1796
                     /locus_tag="VFH83_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485698.1"
                     /translation="METVLEIKGLRTYFNLDEGTLKAVDNVTFQVGTKETLGVIGESG
                     CGKSVTAQSILRIVPPPGKIVDGRILLRRDGGEPVDLTRFDPAGRDIRAIRGKEISMI
                     FQEPMTSLSPVHTIGNQIMEAILLHRTRDTAEARELAIEMLGRVGIPNPTLRIDSYPH
                     QLSGGLRQRAMIAMALSCSPKLLIADEPTTALDVTVQAQILDLMRHLQEQYGMAILYI
                     THDLGVIAEIANKVAVMYLGKVVEYAETREIFHKPLHPYTVNLLKSIPTMGKKARVRL
                     LAIKGTVPIPLNLPKQCGFFSRCPDAMPGKCDVAVPELVEVEPGHWARCFLHNTKREE
                     "
     gene            1804..2817
                     /locus_tag="VFH83_04720"
     CDS             1804..2817
                     /locus_tag="VFH83_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6485699.1"
                     /translation="MEERSRLLDVQGLKKYFPIEKGALRRVSGAIKAVDDVNLFIDAG
                     ETLGLVGESGCGKTTLGRCVLRAIEPTSGKTFFRMDDGNLLDINTLDKKGMRAIRRSM
                     QMIFQDPYSSLDPRMTVLDIIGEPLSVNGIAHGAELEDRVKELVKVVGLEVKYLKRYP
                     HAFSGGQRQRIGIARALALNPKLVVADEPVSALDVSIKAQILNLLQDLQQQYQLTYLF
                     VSHDLSVIEHISDRVAIMYVGKMAELAETEELFNHPRHPYTEALLSAHPKADPDIKMD
                     RIILSGEVANPANPPSGCYFHPRCRYAKDICKEKAPAWEEVAPQHFAACHFAKELSLR
                     GVG"
     gene            2879..3220
                     /locus_tag="VFH83_04725"
                     /pseudo
     CDS             2879..3220
                     /locus_tag="VFH83_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            3413..3931
                     /locus_tag="VFH83_04730"
     CDS             3413..3931
                     /locus_tag="VFH83_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6485700.1"
                     /translation="MHAIGKVHIQQLSDILLDCVEGGASVSFMHPFSRERAAAFWRMV
                     AQGVAHGERVVLVAEDAAGLCGTVQLILAQPENQPHRADVAKMLVHRRTRRHGVGSAL
                     MRAVEETAREHGKTLLVLDTVTGGAAARLYERLGWIRVGDIPGYALFPTGEPCGTTYY
                     YRALGPLTGPQA"
     gene            3933..4850
                     /locus_tag="VFH83_04735"
     CDS             3933..4850
                     /locus_tag="VFH83_04735"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014061349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET6485701.1"
                     /translation="MNDDHRLDPVNDAQVAMHGDELPIDIVLVRRLLAEQLPHLAGRP
                     LAMVRSTGTVNAICRIGNDLSVRLPRVAGWASDLTRELEWLPKLAPLLSLPIPTPVAE
                     GRPTSWYPYPWAVYRWLDGSPYEEALVRDERETARDLAGFIRELRRIDIAGAPRGGRA
                     PLRDLSDATISAMKGSGDRIDVTAALAAWSRSTQTRPWDGNPVWIHGDLLPSNLLVKG
                     GRLCAVIDFGGAGIGDPAADVVPAWSVFSRVGREAFREALDVDDDTWARARGYALHQA
                     ALIIPYYPRTNPAFVAMAERTVAEVLADP"
     gene            complement(5005..6282)
                     /locus_tag="VFH83_04740"
     CDS             complement(5005..6282)
                     /locus_tag="VFH83_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET6485702.1"
                     /translation="MERPSFYWGIGIENCWMAEHDEENRSPKRLLDVFLQMQHYTMWR
                     EDLDRAAALGVNALRYSVPWYRANPRPGVYDWSWIAQPLEYLGRLGIIPIIDLIHYGT
                     PLWMENGVLNHEFPERFAEYASAFARRFRGLVDHYTPHNEPQVSAVFGGLSAYWPPYL
                     RGVDGWLKVGLNVADGMTATAQALRAELPSAVLVSAECLMAPATARVEEALRISVTPQ
                     ERADLEYQVLSFPSSLAYGKVKPASALGRALIRAGVAEDRLERFLQGLAAPDIIGHNY
                     YPGSFAGPEDAMEPGLGRGRSDLAERLRQAHAFFQRPLYLTETSTGFSADQKADWMRA
                     AASVLAELRSGGMPIVGMNWWPLFETIQWDYRDNSKTVAECIRRGGWNNGLYLIEEKP
                     DGTLERQKTAAADAYRQLISAGPPGGRAPGAGR"
     gene            6351..>6836
                     /locus_tag="VFH83_04745"
     CDS             6351..>6836
                     /locus_tag="VFH83_04745"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET6485703.1"
                     /translation="MDIPDDYLERVYAGVLGKLIGVYLGRPFEGWKHERILRELGEIA
                     YYVHERLGVPLVVTDDDISGTLTFVRALEDYGYDRSITAEKIGRTWMNYLIENRTILW
                     WGGMGNSTENTAYLNLKHGIPAPESGSIGRNGALVAEQIGAQIFIDGWALVAPGDPAL
                     AA"
BASE COUNT         1192 a         2207 c         2319 g         1118 t
ORIGIN      
        1 gccgctgatg gcccgggtta cggtaagcat ctggtccgcg ctgggggact ggaacacccc
       61 gcccaccgag cacccgtcca gcatgccctc accgacgtac cccaggaaca gcggcaggtg
      121 tcccgaaccg ccgcccgtgg ccaggccgac cttgcccttc ctcggggacg agaccctcac
      181 caggcaccga gggtcgccgg cggcggccgt cacctggtcc ggatgggcgc ggtagatgcc
      241 ctccagcatg tcgtccacga agcgcttggg atggttgatg aacttcttca tgcggcctcc
      301 cagggcagga tacccgcgcg ccgcgcaacg atcaagcttt tgcgtcgagg agatcttccg
      361 caagtgcggc ccgaacgagc gacctgggct gcctccgatc gtggcggacc ccgatcggct
      421 gggcctggtg gtgaccaacc tgatgcagaa cgccctgcgc tacgctccgg acggtcgccg
      481 ttttccgacg tccccgtgat cattgctcct ctcctacctc gcgcgcaacg ccggtcgcgt
      541 catccctccc cagaccctgg cgacgaacgt ctggggcttg agctacgtgg agagctcgac
      601 catcaagatg tgcgtgcgcc ggcttcggaa gaaactggcg acgataccct ggcccccgag
      661 gcagcggcag gacgttacgg aagcttgctt gcgctggaga ttccgtgcat actcgcgcgg
      721 gacggggtca ggtgggctcc gcgtccctcc ctcgggacgt ccggcaatcc gtccgctgag
      781 gaggcgcgct ggtggagacg gttctcgaga tcaaaggcct gaggacgtac ttcaacctgg
      841 atgagggaac cctcaaggcc gtggacaacg tcacctttca agtcggaacg aaggaaaccc
      901 tcggggtcat cggggaaagc ggctgcggca agagcgtgac cgcccagtcc atcctgcgca
      961 tcgtgcctcc ccccggcaag atcgtggacg gacggatcct gctgcgtcgg gacggcggcg
     1021 agccggtgga cctcacccga ttcgatccgg ccggaagaga catccgggcc attcggggca
     1081 aagagatctc catgatcttc caggagccca tgaccagcct ctcccccgtg cataccatcg
     1141 gcaaccagat catggaggcc attctgctgc accggacgcg agacaccgcg gaggcccggg
     1201 agctggccat tgagatgctg ggacgcgtgg gaatccccaa ccccacgctg cgcatcgaca
     1261 gctacccgca ccagttgagc ggcgggctcc gccagcgggc catgatcgcc atggcgctat
     1321 cctgcagccc caagctcctg atcgcggacg aaccgaccac ggcgctggac gtgacggtgc
     1381 aggcgcagat cctggacctg atgcgccacc tgcaggagca gtacgggatg gccatcctgt
     1441 acatcacgca tgacctcggg gtgatcgcgg agatcgccaa caaggtggcg gtgatgtacc
     1501 tggggaaagt cgtcgaatat gccgaaaccc gcgagatctt ccacaagccc ctgcatccgt
     1561 acacggtgaa ccttctcaaa tcgatcccca ccatgggcaa gaaggcgcgg gtgaggctac
     1621 tggcgatcaa ggggacggtg cccatcccgc tgaacctgcc caagcagtgc gggttcttca
     1681 gccgctgtcc ggacgcgatg ccggggaagt gcgacgtggc ggtgcccgag ctggttgagg
     1741 tggagccggg gcactgggcg cgctgctttc tgcacaacac gaagcgggag gagtgatcgg
     1801 accatggaag agcggagccg tctgctggac gtccaggggc tgaagaaata cttcccgatc
     1861 gagaagggtg cgctgcggcg ggtgagcggg gccatcaagg ccgtggatga cgtgaacctg
     1921 ttcatcgacg cgggggagac gctggggctg gtgggagaga gcggctgcgg gaagactacg
     1981 ctgggccgat gcgttctccg cgcaatcgag cccacctccg ggaagacctt tttccggatg
     2041 gacgacggca atctgctgga catcaacacg ctggacaaga agggcatgcg ggccatccga
     2101 cggtccatgc agatgatctt ccaggacccg tattcgtcgc tggacccgcg gatgaccgtg
     2161 ctggacatca tcggggagcc gctgtcggtg aacgggatcg cccacggcgc ggagctggag
     2221 gaccgggtca aggagctggt aaaggtggtg gggctcgagg tgaaatacct caagcgctac
     2281 ccgcacgcct tcagcggagg gcagaggcag cggatcggga ttgcccgagc cctggctctg
     2341 aaccccaagc tggtggttgc ggatgagccg gtctccgcgc tggacgtctc gatcaaggcg
     2401 cagatcctga acctgcttca ggacctgcag cagcagtacc agctcacgta cctgttcgtg
     2461 tcgcacgacc tcagcgtgat cgagcacatc tcggaccgtg tggcgatcat gtacgtcggc
     2521 aagatggcgg agctggcgga gacggaggag ctcttcaacc atccccggca tccgtacacg
     2581 gaggcgctcc tctccgcgca ccccaaggcg gacccggaca tcaagatgga ccgaatcatc
     2641 ctgtccgggg aggtggccaa ccccgccaac ccgcccagcg gatgctattt ccatccgcgg
     2701 tgccgctacg cgaaggacat ctgcaaggag aaggcgccgg cgtgggagga ggtggcgccg
     2761 cagcatttcg ccgcgtgcca cttcgccaaa gagctgagcc tgcgcggggt gggatgacgg
     2821 ctcggcggcg ctgcgctgct tgccgcccaa gacagccgtc gtgcaggctg gcaaggccat
     2881 gagcatcgag ttcgttcgac tgacgcaggt gccggcttcg gacctggtgt cgctgatgag
     2941 ggacccgagg ctgcggaagc acatgccgct tctgggctcc accttcgatg aggcggcgtg
     3001 ccgagcgttc gttcagcaaa aggaagaagc ctgggacgcg catggatacg gggtgtgggg
     3061 cttctacgtc gatggacgct tcgtcggttg gggcgggctg caggacgaga acggcgatcc
     3121 cgatctggcg ctggtgctgc atccggaaca ctggggcaag gggaggatgc tgttccagga
     3181 gatcgcgcgt cgagctttcg gagagatggg ctttgactac gtcacggcat tgttgcccct
     3241 gacgcggaca accctgttgg ccctggagcg cctcggcttc gagcgggacg ggcaggtgga
     3301 catcggcggt cagacctttt cgcgactcaa gctgacgcgt gcacgccaca ttgccttgcg
     3361 gggtgatcga ggcagcgccg atgggaggga ctctccctgg tcggtccatc ggttgcacgc
     3421 aatagggaag gtccacatcc aacagctatc cgacatcctg ctggattgtg tcgaaggtgg
     3481 ggcctccgtg agcttcatgc atccgttctc ccgggaacgc gccgcggcct tctggcgcat
     3541 ggtggcgcag ggcgtggccc atggcgaacg ggtcgtcctg gttgccgagg atgcggcagg
     3601 cttgtgcggc acggtacagc tcatccttgc gcaaccagag aaccagcctc atcgggccga
     3661 cgtagccaag atgctggtgc accgtcgcac gcgacggcac ggcgtggggt cagccctgat
     3721 gcgcgccgtt gaagagaccg cccgggagca tggaaagacc ctcctggtgc tggacaccgt
     3781 gaccggtgga gcggcggcgc gcctctacga gcgcctgggg tggatccgcg tgggcgacat
     3841 ccccggctac gcgcttttcc cgacgggtga accgtgcggc accacctact actaccgcgc
     3901 cctggggcct ctcacgggac cgcaggcata gagtgaacga cgatcatcgg ctggatccgg
     3961 tcaacgacgc tcaggttgcg atgcacggcg acgagcttcc catcgacatc gtgctggtcc
     4021 ggcggttgct ggctgagcag ttgccccatc tcgccggccg accgctcgcc atggttcgtt
     4081 cgactggcac ggtcaacgcg atctgcagga tcggaaacga cctctccgtt cggctgccgc
     4141 gggtagctgg gtgggcaagc gacctgacga gggagctcga gtggctgccg aagctcgcgc
     4201 cgctcttgtc ccttcccatc cccacgccgg tagccgaggg tcggccgacg agctggtacc
     4261 cctacccctg ggcggtgtac cggtggctcg acggctcacc gtatgaggaa gccctggtgc
     4321 gggacgaacg ggagacagcc cgcgacctcg ccgggttcat acgcgagctc aggcggatag
     4381 acatcgcagg ggcgccgcgg ggcggccgcg cgcctttgag ggatctgagc gacgcaacga
     4441 tttccgcgat gaagggcagt ggggacagaa tcgacgtcac ggccgccctc gcagcctggt
     4501 cgcgatccac ccagacccgg ccatgggacg ggaatcccgt ctggatccat ggtgacctgc
     4561 tcccatccaa cctcctggtg aaaggcggca ggctctgcgc cgtgatcgac ttcgggggag
     4621 ccgggatcgg agatcctgcc gccgacgtgg ttccggcgtg gagcgtgttc agcagggttg
     4681 gcagagaagc gtttcgagag gcgctggacg tcgatgatga cacgtgggcc cgggctcgcg
     4741 gatacgcgct gcatcaagcc gcgctgatca taccgtacta cccgcgaacc aaccccgcgt
     4801 ttgtggccat ggcagagcgc accgttgccg aggtgctggc ggacccgtag cagcggccct
     4861 tcctggcgcg gctcgtgtcg gcagcgggtc ccgagggcga gtacctgcta tcgtacctga
     4921 acaagccggg atggtccgag gcggaggtca gcgatctgta tggcaggttg aaggcgacca
     4981 cggcgggtcc gccatgctga cgctctatcg gccggcccca ggagcgcgac cccccggggg
     5041 cccggcggag atgagctgcc ggtaggcatc cgcggccgcg gtcttctgtc gctccagcgt
     5101 gccgtcgggc ttctcctcga tcaggtacag gccgttgttc cagccgccac ggcggatgca
     5161 ctccgccacg gtcttgctgt tgtcgcgata gtcccactgg atcgtttcga agagcggcca
     5221 ccagttcatt ccgacgatcg gcatcccgcc gctccgcagc tccgccagca ccgatgccgc
     5281 ggcccgcatc cagtccgcct tctgatcggc gctgaatccc gtgctggtct ccgtcaggta
     5341 caacggcctc tggaagaacg cgtgggcctg gcgaagcctc tccgccagat cggatcggcc
     5401 ccgccccagc ccgggctcca tcgcgtcctc aggccccgcg aagctgccgg ggtagtagtt
     5461 gtgtccgatg atgtcgggcg ccgccaaccc ctgaaggaac cgctccagcc ggtcctcggc
     5521 aaccccggcg cggatcagcg cgcggcccag ggcgctggcc ggtttcacct tcccgtaggc
     5581 cagagaggac gggaacgaca ggacctggta ctcgagatcg gcccgctcct gcggcgtcac
     5641 gctgatccga agcgcctctt ccacccgcgc agtcgccggg gccataaggc actcggccga
     5701 caccaggacc gcggacggaa gctcagcgcg aagggcctgg gccgtggccg tcattccgtc
     5761 cgcgacgttc agaccgacct tcagccaccc gtcgacgcct cgcaggtacg gcggccagta
     5821 cgcggaaagc ccgccgaaca ccgcgctcac ctgcggctcg ttgtggggcg tgtagtgatc
     5881 caccaggccc ctgaaccgcc gggcaaaagc ggaggcgtat tcggcgaagc gctcggggaa
     5941 ctcgtggttg agcacgccgt tctccatcca gagcggcgtc ccgtagtgga tgagatcgat
     6001 gatggggatg atcccgagcc gtccgaggta ttccagcggt tgggcgatcc agctccagtc
     6061 atagacaccg ggccggggat tggcccggta ccaggggacg ctgtaccgca gcgcgttcac
     6121 gccaagggcg gcggcccggt ccaggtcctc gcgccacatg gtgtagtgct gcatctggag
     6181 gaacacgtcc agaagccgct tgggcgatcg gttctcctcg tcgtgctccg ccatccagca
     6241 gttctcgatg ccgatgcccc agtagaacga cggccgctcc atggtacccc tgggctcgta
     6301 ccctacactt tcttgacagg gcggggaagg cgtcctagga tgcagccacc atggacatcc
     6361 ctgacgatta cctggagcga gtctacgcgg gcgtgctggg caagctgatc ggcgtctacc
     6421 tgggccgccc tttcgaggga tggaagcacg agcgcatcct gcgagagctc ggggagatcg
     6481 cctactacgt ccacgagcgg ctgggggtcc ccctggtggt cacggatgac gacatcagcg
     6541 gcaccctcac cttcgtgcgc gccctggaag actacgggta cgaccgctcc atcaccgcgg
     6601 aaaagatcgg tcggacctgg atgaactacc tgatcgagaa ccggacgatc ctttggtggg
     6661 gcgggatggg caactccacc gagaacaccg cgtatctgaa tctcaagcac ggcatccccg
     6721 cgccggagag cggctccatc ggccgcaacg gcgccctggt ggcggagcag atcggcgctc
     6781 agatcttcat cgacggctgg gcgctggtgg cgccgggaga tcccgccctg gcagcg
//
LOCUS       DASRZS010000193         2395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_875992, whole genome shotgun sequence.
ACCESSION   DASRZS010000193 DASRZS010000000
VERSION     DASRZS010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875992"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFH83_04750"
     CDS             <1..287
                     /locus_tag="VFH83_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HET6485704.1"
                     /translation="CPYFVVLTDPTTAGVLASFAMLGDVILAEPGALMRFAGARVVAG
                     TIREKVPENFPLSQYHAERGFIDLVVPRQEMKKTLGFLLATHAPASGGKV"
     gene            284..1261
                     /locus_tag="VFH83_04755"
     CDS             284..1261
                     /locus_tag="VFH83_04755"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017152990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HET6485705.1"
                     /translation="MSDSSNTNLREKLMELKTLAAKSNIDIGRELAELDAKLHAASHP
                     ENEAWRRVELARHPERPTTLQYAERMFDDFLELHGDRAFGDDPALVGGIAVFGGRPIT
                     FFGHQKGHNMKENLRRNFGMAHPEGYRKALRLAHQAAKFGRPILTFVDTPGAYPGVSG
                     EDRGISQAIAENMKAFCELPVPIVVTVLGEGGSGGAIGIGLGDTILMMENAYYSVITP
                     EGCASILLRDASKARLSASLLKLTPQDMLRFGVIDRVIPEPPGGAHQDLATAAATLKA
                     AIAESLEALSHKGTEALLAQRHEKFLSMGMFQEPEARRRSLLQRLRDFF"
     gene            1300..1983
                     /locus_tag="VFH83_04760"
     CDS             1300..1983
                     /locus_tag="VFH83_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET6485706.1"
                     /translation="MSRFIWFDLGYTLLYEPRELAYQRVLAEHGHAFTLEALERAFHV
                     SDKLVMRLYPEVFGHDPETFMPMFLGLLNHRLGVRLELCRAWQRLKEERLALDVRWLP
                     FPNVKTVLADLERRGVRMGVITNWDPTARAILQGHGLDCFFEQIVISSEVGREKPDPA
                     IFELAMRRAAVGAAESLYVGDNYYVDAVGARGVGMRYLIVNRFGTLGVEEIREPVLIH
                     DVSDVISRL"
     gene            1993..>2395
                     /locus_tag="VFH83_04765"
     CDS             1993..>2395
                     /locus_tag="VFH83_04765"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485707.1"
                     /translation="MRLLLFSLRRLGRLLLTVLLISTIVFFVIRVIPGDPALVIAGVD
                     ASPDAVAAIRVRIGTDKPLAVQYGTWLLDVASLRFGTSLATGQPVAALVFQRFPLTLS
                     LAMLGLLLSVAIAVPLGIASAVRRWSGWDYLG"
BASE COUNT          373 a          797 c          797 g          428 t
ORIGIN      
        1 gctgcccgta cttcgtggtc ctcacggacc ccaccacggc aggcgtcctg gcttccttcg
       61 ccatgctggg ggacgtcatc ctggccgagc cgggcgcgct catgcggttc gctggcgctc
      121 gggtggtggc gggcaccatc cgggagaagg tccccgagaa cttcccgctc tcccagtatc
      181 acgcggagcg tgggttcatc gacctggtgg tgcctcgcca ggagatgaaa aagaccctgg
      241 gcttcctcct ggcgacccat gccccggcat cgggaggcaa ggtatgagcg actccagcaa
      301 caccaacctc cgagagaagc tcatggagct caagaccctg gcggcgaaga gcaacatcga
      361 tatcggccgt gagctggcgg agctggacgc caagctgcac gccgcctccc atccggagaa
      421 cgaggcctgg cgccgcgtgg agctggcccg ccatcccgag cggcccacca cgctccagta
      481 cgccgaacgg atgttcgacg acttcctgga gctccacggg gaccgcgcct ttggagacga
      541 cccggccctg gtcggcggga tcgcggtctt cggcggccgc cccatcacct tcttcggcca
      601 ccagaagggc cacaacatga aggagaacct gcggcgcaac ttcgggatgg cccaccccga
      661 gggctaccgc aaggcgctcc gcctggcgca ccaggcggcc aagttcggcc ggcccatcct
      721 cacgttcgtc gacacgccgg gggcctaccc gggcgtgagc ggggaggacc gcggcatcag
      781 ccaggccatc gcggagaaca tgaaggcgtt ctgcgagctg ccggtcccca tcgtagtcac
      841 ggtcctggga gaggggggat cgggcggcgc catcggcatc gggcttggcg ataccatcct
      901 gatgatggag aacgcctact actcggtcat cacgcccgag gggtgcgcct ccatcctcct
      961 tcgggacgca tccaaggcca ggctctccgc gtccctcctc aagctgaccc cccaggacat
     1021 gctccgcttc ggagtgatcg accgcgtcat ccccgagccc cccggcggag cgcaccagga
     1081 tctggccacc gcggccgcga cgctgaaggc cgcgatagcg gagtccctgg aggcgctttc
     1141 tcacaagggc accgaggcgc tccttgccca gcgtcacgag aagttcctga gcatggggat
     1201 gttccaggag ccggaggctc gccgcaggag ccttctgcag cggctgcggg acttcttcta
     1261 gtcgcgcgca ggccggtcct ggaccgcgag cgaaacccca tgagccgttt catctggttc
     1321 gacctgggct atacccttct gtacgagcct cgtgagcttg cctaccagcg ggtgctggcg
     1381 gaacacgggc acgctttcac gctggaagcg ctggagaggg ccttccacgt ctcggacaag
     1441 ctcgtcatga gattgtatcc ggaggtcttc ggccacgacc ccgagacgtt catgcccatg
     1501 ttcctcggcc tgctcaatca ccggttgggt gttcgcctgg agttgtgccg ggcctggcag
     1561 aggctcaagg aggagcggct cgctctggac gtgcgctggc tcccgttccc caacgtaaag
     1621 acggtgctgg ccgatctgga gcggcgtggc gtgcgtatgg gcgtcataac caactgggac
     1681 ccgacggcgc gcgcgatcct ccaggggcac gggctggatt gcttcttcga gcagatcgtg
     1741 atctcctccg aggtggggcg cgagaagccg gatcccgcca tcttcgagct ggccatgagg
     1801 cgagccgccg tgggggcggc ggagagtctc tacgtggggg ataactatta cgtcgatgcc
     1861 gtgggggccc gcggggtcgg catgcggtac ctcatcgtga atcggttcgg gactctgggg
     1921 gtcgaggaga tccgggagcc cgtcttgatc cacgacgtct ctgatgtgat cagccggctg
     1981 taggcaaccg tcatgaggct gctccttttc agcctgagaa ggcttggaag actgctcctc
     2041 acggtcctgc tgatttccac catcgtcttt ttcgtgatcc gcgtgatccc gggcgatccg
     2101 gccctggtca tcgccggggt ggacgccagc cctgatgccg tggccgccat ccgggtcagg
     2161 attggcacgg acaaacctct ggcggtccag tacggcacct ggttgctgga cgtcgcctcc
     2221 ctccggttcg ggacctccct ggccaccggg cagccggttg cggcgctcgt gttccagcgg
     2281 ttcccgttga cgctgagcct cgccatgctt ggacttctcc tttccgtggc tattgcggtt
     2341 ccgctgggaa tcgcgtccgc ggtccggcgg tggtccggct gggactacct gggca
//
LOCUS       DASRZS010000194         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_876617, whole genome shotgun sequence.
ACCESSION   DASRZS010000194 DASRZS010000000
VERSION     DASRZS010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876617"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFH83_04770"
     CDS             <1..248
                     /locus_tag="VFH83_04770"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HET6485708.1"
                     /translation="VRKTEDYSYSGQHLRTTAFPVYQQVGTRYVPQTVVILDELRGAK
                     INGAFVKEKTTYTIERPSLQPLPDATFTKAWLENLSE"
     gene            241..1650
                     /locus_tag="VFH83_04775"
     CDS             241..1650
                     /locus_tag="VFH83_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485709.1"
                     /translation="MNKWIAVAAAFLLAAGRLLAQDAAPSSPSSDDIDNLFDGNGTSG
                     NNGGTTSSGSPGTDAGTATPPAGPDVRADDPTRDNRLHFLSDLSIYGLGGVGWKTDSN
                     LGEGFDGLSGEGSGSLSASLGMDFRPAPELRVRGTLSYNFPQAGTQLSQLEVDYSVRN
                     AVFFSLGIFGYTWGNAQFFQYGNLPARALPGWSGVANVPLWQQLNIITTTVVANYPAS
                     VKMYVPFGVNGFTFISRFDITNYGFPDKTAPDPRYAGYGLQLDLVTGPVQWTLSGYAQ
                     QLLAPRTSLDIKTSILGFDVALEGIIAYPVNLETSDPQQFFTAIPTSGGGIFVGGFLQ
                     RIYPTAVVSLFREWTDPHIRLYAEYGYNGERDATTSWLADATGPGGHNTALALRFSNL
                     FSSDISLNAVWQQNWSDWSGLVSAFLEISPVALTTIQLGPVFVFGPENGVVPNARLVP
                     GDKRLELVLLVKVSDSFRQ"
     gene            1771..>2183
                     /locus_tag="VFH83_04780"
     CDS             1771..>2183
                     /locus_tag="VFH83_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HET6485710.1"
                     /translation="MQATQSRYGTTSDGRVVTEFVLESARGVRVHIIDLGATVTAVMM
                     PDRAGKSANICLGHDNLAGWEKNTTFFGCIVGRVANRIRDGRFELDGKVYSVAKNVLG
                     KHHLHGGIKGFDKYVLKAKLFKKGDSAGVRFTHTSP"
BASE COUNT          385 a          757 c          661 g          380 t
ORIGIN      
        1 tggtccgcaa gaccgaggac tacagctact cggggcagca ccttcgcacc acggcgttcc
       61 ccgtctacca gcaggtgggc acccgctacg tcccgcagac cgtggtcatc cttgacgagc
      121 ttcgcggcgc aaagatcaac ggggcctttg tgaaggaaaa gaccacctac accatcgagc
      181 ggccatccct gcagcccctg ccggacgcga cgtttaccaa ggcgtggctg gagaacctga
      241 gtgaataaat ggatcgccgt cgctgccgcc ttcctgcttg cggccggaag actcctggcg
      301 caggatgcgg ccccgtcctc cccgtcttcc gacgacatcg acaacctgtt cgatggcaac
      361 gggaccagcg ggaacaacgg cggaaccacc agctcgggat ctcccgggac ggacgcggga
      421 accgcgacgc ctcccgcagg gccggacgtg cgcgcggacg atcccacgcg ggacaaccgc
      481 ctgcacttcc tgagcgatct ctccatctac gggcttggcg gcgtggggtg gaagacggac
      541 tcgaaccttg gcgaggggtt cgacgggttg tccggtgaag gcagcggctc cctttccgcc
      601 agtctcggga tggacttccg gcccgccccc gagctgcgcg tccgagggac actgagctac
      661 aacttcccgc aggccggtac ccagctcagc cagctcgagg tggattactc cgtccgcaac
      721 gcggtgttct tcagcctggg tatcttcggc tacacgtggg gcaacgcgca gttcttccag
      781 tatggcaacc tgccggcccg cgcgctgccg gggtggagcg gggtggccaa cgtcccgttg
      841 tggcagcagc tcaatatcat caccacgacc gtggtggcca attatcccgc ctcggtgaag
      901 atgtacgtcc cgttcggcgt caacgggttc acgttcatct cgcggtttga catcacgaac
      961 tacggttttc cggacaagac ggcgcctgac ccccgctacg ccggctacgg tctgcagctc
     1021 gacctcgtca ccgggcccgt ccagtggacc ctgagcggct acgcccagca actgctggcg
     1081 ccccgcacgt cgctggacat caagacgagc atcctcgggt tcgacgttgc cctggaagga
     1141 atcattgcct accccgtcaa cctggagact tccgatccgc agcagttctt tacggcgatc
     1201 ccgacttcgg gcggcgggat ctttgtcgga ggctttctcc agaggatcta tcccaccgcc
     1261 gtggtgagct tgttccgaga gtggactgat ccccacatcc gcctgtacgc ggagtacggc
     1321 tacaacggcg agcgggatgc aaccacgtcc tggctcgcgg atgccaccgg ccccggcggg
     1381 cacaacaccg cactcgcgct gcgtttctcc aacctcttct cgagcgacat ctccttgaac
     1441 gccgtctggc agcagaactg gtccgactgg tctgggcttg tatctgcctt cctcgagatc
     1501 tcgcccgtgg ccctgaccac catccagctc ggcccggtgt tcgtgttcgg ccccgagaat
     1561 ggcgtggtcc cgaacgcccg cctcgtgccc ggggacaagc gcctcgagct ggtgctcctg
     1621 gtcaaggtca gcgacagctt ccgccagtag cctctcaccg cgggccagct gccgctggcc
     1681 cagcaccctc ccccgtctcc ccaaccgatg gactctggac gccccggtcc tgcatactcc
     1741 tcccataccc gctttcgcag gaggaacgtc atgcaggcaa cacaatcgcg atacggcacc
     1801 acgtccgacg gcagggtcgt cacggagttc gtcctggaaa gcgcgcgcgg cgtgcgtgta
     1861 cacatcatcg accttggggc cacggtcacc gcggtgatga tgcccgaccg cgcgggcaag
     1921 tcggccaaca tctgcctggg ccacgacaac ctggccggct gggaaaagaa caccaccttc
     1981 ttcggctgca tcgtgggccg cgtggccaac cgcatccgcg atggtcgttt cgagctggac
     2041 ggcaaggtgt acagcgtggc gaagaacgtg ctgggaaagc accacctgca cggcggcatc
     2101 aagggtttcg acaagtacgt gctcaaggcg aagctcttca agaagggaga ctccgccggg
     2161 gtgcgcttca cgcacaccag ccc
//
LOCUS       DASRZS010000195        12615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5494414, whole genome shotgun sequence.
ACCESSION   DASRZS010000195 DASRZS010000000
VERSION     DASRZS010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 12615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12615
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5494414"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VFH83_04785"
     CDS             <1..1094
                     /locus_tag="VFH83_04785"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET6485711.1"
                     /translation="EDGMLVEVGLAGICGTDIHIMENADRPEWSKGLPFTLGHEISGR
                     VAKIGKKAQEGLFCDDKLVEGDKIAFYAFLPCGNCWWERRLGPDHNLLCTQPKPGYFM
                     NPDQWPHFTSGWSEYLYIHPGSWVWKAPDGMPFEQIVLTEPFSMGIRGVAKATALPAW
                     KNLDMLSFGGTVAVLGAGAIGVLTAVAAKIAGAGQVILIGGPRKNLDIAKQIGAADVF
                     IDIDSTQPAERVEQVKKISEGGVGADVVIEAAGVPVAFIEGLDMVRKEGTYVEMGLLI
                     DDGRTVPLNVARSIVQKDLTLYGVTNQPAHDFTKALRCMKNFKNRFDFSKVVTDVFAL
                     KDVEKAYTKAKHPQEKGIKIALKGKGYGK"
     gene            1108..2094
                     /locus_tag="VFH83_04790"
     CDS             1108..2094
                     /locus_tag="VFH83_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6485712.1"
                     /translation="MKYKQLGGSGIKVAEICLGTMTFGREADESTARTIIGQYLEAGG
                     NFIDTADVYGAQRGDTETVVGKLLKGRRKDIILATKTGFGAGMNNEGMSRRHLINAVD
                     GSLSRLQTDWIDVFYLHIYDRSTPFEETLSTLDALVKSGKIRYIGVSNFKAYQIMKML
                     WVSDARSLVRFVVYQPQYNLLEREIEREFVPICQTEGLGMVTWAPLAGGFLSGKYKPS
                     TTEGRLRRVGRPDTDSWENRVNDFSLGISKAVDAIAQKRKVPCSQVALNWLRSKPWVT
                     APIIGARTAEQYAENAGCLGWELDAGEGETLDKVSTTPKPYPYHFVDWLQRG"
     gene            2107..3921
                     /locus_tag="VFH83_04795"
     CDS             2107..3921
                     /locus_tag="VFH83_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485713.1"
                     /translation="MLSFYTKHEALNVPVLEERWAGAPRTGYEQMAAWIRERVAEKGA
                     GRKTVRVALDGYLGVAWDPLVRTLLARLADLGVRCVYQAEIRSCFQPQVAVEARVQGC
                     FANDPPWGRVYDGTLADFMDAGALTRVRGELDRISASTRREATIALVYGTGAAVAQLL
                     DAFDLVGYVDLTKKEMLRRFENQEALLLGAETAEPNEKRVARRLPYVDFPVLHPHKRA
                     VLKRMDWIILADSTEAPVLVPSDAYRAILASVANQPFRTLPYYFPRIWGGTRMVKLRG
                     LPMKRCSFCIEVAPRNQSVRILVGNTVMEVQFLNLIWGNPLDLMGKASVDRFGEYFPI
                     TLNYDDNYHGGSLAIQNHPGRQYMRERFNEFDTHDEMYYVVRAWKGAKTYHGLRDGCN
                     LEELRALAARAEKEGVPFDYEAYVNSWDFPGTVHDLYLHPAGTIHASGVNQLVLEIDF
                     DGSREGQEYTFHLYDYLRKDLDGTLRGIHLDHFFHELRAERTRSWIPGNLKQEPKLLR
                     SERRGAAEYVIGDYHEMYYRVHRLEFRTRITDATNGQFHILSLVAGDRIQIRSLEHPE
                     RSYEIAYSESVIVPASVGAYEVVNRGRGACKVTKAFLR"
     gene            3938..4912
                     /locus_tag="VFH83_04800"
     CDS             3938..4912
                     /locus_tag="VFH83_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6485714.1"
                     /translation="MTAVPPRGLVAADMGGSFLKFAVVVSEEPEHATADRTFGRVPMS
                     DTEERSVADVLADAVGQALRTADRLGIPVSGIGVCSPGPFDWHRAVSLMKHKFLSIQG
                     QELWPLVRARLPRVAAIAMRLVPDSYAFAAGERDHGAARDYSRCLYITLGTGIGSALS
                     ENGRIFLHLPDKSPDGSIWCAPYRDGITEDYVSKKAILSGYAGARASAAPRDRAPAAP
                     PGDRAPGLDVADIAARARAGEEAALDVFGRFGRHLGAALRAYASGFDPQCIVIGGGLA
                     PCFPLFESELKRETREMPGLSAIAVSTLGEIGPLLGIAATYEEGYREP"
     gene            complement(4885..5964)
                     /locus_tag="VFH83_04805"
     CDS             complement(4885..5964)
                     /locus_tag="VFH83_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6485715.1"
                     /translation="MATIYDVARESGYSPTVVSHALNNNPRVSEKARAAVLVAAKRLH
                     YRPNLLARELGLNKTKLIGIIVRDIIYPFYPSIVEGIEETAFSHGYTTILCNSAGSIE
                     REAIYLDVLTRRKAAGIIVGPVQSGPDNTVLFQELKESGVPIVFVDGNLKRVKTDFIL
                     TDGVKGAYKAVRHLVELGHNRIGCVEPASGEPNRLAGYRKALEDSHIDFREEYVARRK
                     GDRGDQGKIGNEVDGYEGTRQLLQLRYPPTAIFAINDVMAFGAEKAIREFGLRIPADI
                     SLVGFDDVLFSSFLGVPLTTVKQPTHAIGRTAATILISRIEQQGRQKRRRHISIEPDL
                     VVRESTGPAPRKARRRAQGSRYPSS"
     gene            6487..8229
                     /gene="thrS"
                     /locus_tag="VFH83_04810"
     CDS             6487..8229
                     /gene="thrS"
                     /locus_tag="VFH83_04810"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET6485716.1"
                     /translation="MDEAHQEKLLRIRHSFSHVMAEAVLQMFPRARLAIGPAIDEGFY
                     YDFELPRALATEDLAVLEERMRAIIAGDHEFRRHSVTREEARRRFADQPYKLELIAEL
                     PEGEDISTYTQDTFTDLCRGPHVSSTRELPPDAFKLTSVAGAYWRGDEKNPMLQRIYG
                     TAWETRKELETWLKRREEIEKRDHRRLGKDLDLYSVHEEAGAGLVYWHPKGARIRLAI
                     EDFWRREHLRHGYELVYSPHIGKSWLWETSGHLGFFKESMYAPLTIDEASYYLKPMNC
                     PFHLMIYKDRQRSYRELPLRWAELGTVYRYERSGVLHGLLRVRGFTQDDAHIICPPER
                     IEDEILEVLRFSLKIWKAFGFQDIKAYLATRPAHAVGNDESWQKAIASLRRAAEVEQV
                     PLVMDEGGGAFYGPKIDLKVRDALGREWQMTTIQFDFNEPELFGITYVAADNTPRRPF
                     MVHRALLGSMERFFGVLIEHYAGAFPGWLSPVQVMAIPVSAAFVPYAAEVVGALKARD
                     VRAEVDGGDDRMNAKIRNAQNQKIPYMLVLGEKEQSARTVSLRTRSGEQRNQIPLAEL
                     VSLIEAEVRPPTLD"
     gene            8277..9611
                     /locus_tag="VFH83_04815"
     CDS             8277..9611
                     /locus_tag="VFH83_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HET6485717.1"
                     /translation="MIEMIVKRDGRVVPFARRKITQAVYRAAVACGGRDQAEAELVTD
                     DVLALLQKRTYQGTWPAVEEVQDLVEKCLIERGHARTAKAYILYRYEHALKRAGRESL
                     TYSRENIPYRKLWEALSWATDHDCVTLARLTSIIQSGALPDLIQASEDFYEQELESAA
                     EKILARRDELRIVIIAGPSSSGKTTTTLKVRDKLQAAGMRTLPISLDNYFFDLKMHPR
                     VGENDYDFETPQALDLALINRHLHALASGEAVEVPRYNFKTGQREGISGTLRLETDQV
                     LLIDSLHGLFPEMTSAVAEEKKFRLYIETLSQIKGPDGRYLRWADIRMLRRVVRDMQF
                     RGYSPRQTIRHWHLVRRSELRYIVPELRRAHAIVNSFLPYELPIMKARVGESLRGLAE
                     EFGRDPEAQDAQERAARVMAVFEQLPAVQDEGLVPGRSLLREFIGGSTYRYE"
     gene            9627..10478
                     /locus_tag="VFH83_04820"
     CDS             9627..10478
                     /locus_tag="VFH83_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485718.1"
                     /translation="MDGKSMVSALLASPLPFAVLGGIFVGMAVSRATRRTDRSRDPAR
                     ARTGKWVGFCVYLSLAVVLGILAVFLPGARKIADIRVAYVAAAAAILSFAGTRFRLAL
                     GIPIVVILVLAVTTGALFLRSIRAFTGETTIATVRVISAGQDSMRLQLLPRDGEPAMV
                     TMQGEYFAPIVKVVIFNDLWVFLGAHTWYRFEGLTSFRMVKEGDGYRLRQGTTDYYLP
                     RAAGISERLWSLFEANERSIPGVKTVQIEMDLKRAEPTEPAAQELHSYEIRVQNDGGV
                     EVVPVNG"
     gene            complement(10475..11293)
                     /locus_tag="VFH83_04825"
     CDS             complement(10475..11293)
                     /locus_tag="VFH83_04825"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET6485719.1"
                     /translation="MRPVPGIRRSVLAHGIAFLVLQEAIIAALTFGYGMELPRAALFA
                     AVALAYHALLTGVLLARWEDFRTEPAGLRLERVNLANTLSFGRLSSIPTILYMVMQAP
                     AYRVLPVTLPFVCVVFATDFVDGMVARRRGEITFVGRYLDSSSDYVTIIAMSILFYIN
                     GLIPLWFFILVLARLVLFALGMAVLALREGKANPLATFLGKVSIFALMVLYLAEIAGV
                     FGVPWIGNPTVVSILEYVVAAVVVVSMGDKVVFLWGRFAEVAVGRGKRRENTGS"
     gene            11427..11999
                     /locus_tag="VFH83_04830"
     CDS             11427..11999
                     /locus_tag="VFH83_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET6485720.1"
                     /translation="MVPMVLTVLRMVLAPVFFGFYALASAGSPSFVIGVWLVFAAIEI
                     SDVLDGHLARSLRQETEAGKVLDPLADSVSRLTYFLCLTGTGIMPLWVLLILIYRDLG
                     VAYIRVMVSRSSVLLPARLSGKLKAWVYAFTGGVGIAVFSVRRLGWLPSLQNVAGTIS
                     LGLCVLAALIAVWSLADYAAFFLRNFRRTS"
     gene            12254..>12615
                     /locus_tag="VFH83_04835"
     rRNA            12254..>12615
                     /locus_tag="VFH83_04835"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2230 a         3981 c         4284 g         2120 t
ORIGIN      
        1 aggaagacgg catgctggtg gaggtgggtc tcgccgggat ctgcgggacg gacatccaca
       61 tcatggagaa cgcggatcgg cccgagtgga gcaagggtct gcccttcacc ctgggtcacg
      121 agatctcggg ccgcgtggcc aagatcggga agaaggcgca ggaggggctc ttctgcgacg
      181 acaagctcgt ggagggggac aagatcgcct tctacgcgtt cctgccctgc gggaactgct
      241 ggtgggagcg ccgcctgggc ccggaccaca acctgttgtg cacccagccc aagcccggct
      301 acttcatgaa cccggaccag tggcctcact tcacctcggg ctggagcgag tacctctata
      361 tccatccggg aagctgggtc tggaaggctc cggatggcat gcccttcgag cagatcgtcc
      421 tcaccgagcc gttctccatg gggatccgcg gcgtcgccaa ggccaccgcg ctgccggcct
      481 ggaagaacct ggacatgctg agcttcggcg gcacggtagc cgtcctgggc gctggggcca
      541 ttggcgtgct caccgccgtg gcggccaaga tcgccggcgc cgggcaggtg attctcattg
      601 ggggccccag gaagaacctg gacatcgcaa agcagatcgg cgcggcggac gtgttcatcg
      661 acatcgattc cacccagccg gcagagcgcg tcgagcaggt gaagaagatc tccgagggcg
      721 gtgtgggggc ggacgtggtc atcgaggcgg ccggagtccc ggtggcgttc atcgaggggc
      781 tggacatggt tcgcaaggag ggcacctacg tcgagatggg actcctcatc gacgacggaa
      841 ggacggtccc gctgaacgtg gctcggagca tcgtccagaa ggacctcacg ctgtacgggg
      901 tgaccaacca gcccgcgcat gacttcacca aggccctgcg gtgcatgaag aacttcaaga
      961 accgcttcga cttctccaag gtggtcacgg acgtgttcgc cctgaaggac gtggagaagg
     1021 cctacaccaa ggcgaagcat ccgcaggaga agggcatcaa gatcgccctc aagggcaagg
     1081 gatacggaaa atagccggga ggatggcatg aagtacaagc agctcggcgg ctccgggatc
     1141 aaggtcgcgg agatctgcct ggggaccatg acctttggtc gggaagcgga cgaatcgacc
     1201 gcccggacca tcatcggtca gtacctggag gccggaggaa acttcatcga cacggcggac
     1261 gtgtacggcg cgcagcgcgg ggataccgaa accgtggtcg gcaagctcct caagggcagg
     1321 cgtaaggaca tcatcctggc gaccaagacg ggcttcggcg ccggcatgaa caacgagggg
     1381 atgtcccgcc gccatctgat caacgccgtg gacggcagcc tctcccgtct ccagacggac
     1441 tggatcgacg tcttctatct gcatatctac gaccgttcca cgccgttcga ggagacgctc
     1501 tcgacgctgg atgccctggt gaagtccggg aagatccgct acatcggcgt ctccaacttc
     1561 aaggcgtacc agatcatgaa gatgctctgg gtgagcgacg cgcgctccct ggtgcgcttc
     1621 gtggtctacc agccccagta caacctgctc gagcgcgaga tcgagcggga gttcgtcccg
     1681 atctgccaga cggagggcct gggcatggtc acctgggcgc cgctggccgg cggctttctc
     1741 tccgggaagt acaagccatc caccacggaa ggcaggcttc ggagagtggg ccggccggac
     1801 acggatagct gggagaaccg ggtcaacgac ttcagcctgg gtatctccaa ggccgtggac
     1861 gccatcgccc agaagcgcaa ggtgccatgc tcccaggtgg ctctgaactg gctgcggtcc
     1921 aagccgtggg tgacggcgcc catcatcggg gccaggaccg cggagcagta cgccgagaac
     1981 gccgggtgcc tggggtggga gctcgatgcc ggggaagggg agacgctcga caaggtcagc
     2041 accaccccca agccgtatcc ctaccacttc gtggattggc tccagagggg ctgatcggga
     2101 gaccggatgc tcagcttcta tacgaaacac gaagcgctca acgtgcccgt gctggaggag
     2161 cgctgggccg gcgctcctcg gaccggctac gagcagatgg ccgcctggat ccgggagcgc
     2221 gtcgcggaga agggggcggg gcgaaagacg gtccgcgtcg ctttggacgg ctacctggga
     2281 gtggcctggg accctctggt gcggacgctg ctggcccgtc tcgcggacct tggagtccgg
     2341 tgcgtctacc aggcggagat tcggtcgtgc ttccagccgc aggtggcggt ggaggcccgg
     2401 gtccagggct gcttcgcgaa cgacccgcca tggggcaggg tctacgacgg gacgctggcg
     2461 gacttcatgg atgccggcgc cctgacccgg gtccgcgggg agttggatcg gatctcggcg
     2521 agcaccaggc gcgaagcgac catcgcgctc gtctacggga ccggggcggc cgtggctcag
     2581 ctcctggatg cgttcgacct cgtcggctac gtggacctca ccaagaagga gatgctccgc
     2641 aggttcgaga accaggaggc gctcctcctg ggcgcggaga ccgcggaacc caacgagaag
     2701 cgggtggccc gtcgccttcc gtacgtggac tttcccgtcc tgcacccgca caagcgggcg
     2761 gtcctcaagc ggatggactg gatcatcctt gcggacagca ccgaggcgcc cgtgctggtt
     2821 ccctcggatg cctaccgagc catcctggcc tcggtcgcaa accagccgtt tcggacgctg
     2881 ccgtactact tcccgcgaat ctggggcggc acccgcatgg tgaagctgcg cgggctcccg
     2941 atgaagcgct gctccttctg tatcgaggtg gctcccagaa accagagcgt ccggatcctg
     3001 gtggggaaca ccgtgatgga ggtgcagttc ctcaacctga tctggggtaa tcccctggac
     3061 ctcatgggaa aagcctccgt cgatcggttc ggagagtact tccccatcac gctcaactac
     3121 gacgacaact accacggcgg aagcctggcc atccagaacc acccgggccg gcagtacatg
     3181 cgggagcggt tcaacgagtt cgatacgcac gacgagatgt actacgtggt ccgggcctgg
     3241 aagggcgcga agacctacca cggtctgcgg gatgggtgca acctggagga gctgcgcgcc
     3301 ctggcggcgc gggccgaaaa ggaaggcgtg ccgttcgact acgaggccta cgtcaatagc
     3361 tgggattttc ccggtacggt gcacgacctc tatctccacc ccgcgggcac gatccacgca
     3421 tccggcgtga atcagctcgt gctggagatc gacttcgacg gcagccgtga gggccaggag
     3481 tacaccttcc atctctacga ttacctgaga aaggacctgg acggcacgct ccgcgggatc
     3541 cacctggatc acttcttcca cgagcttcgc gccgagcgta cgcggtcctg gatcccgggg
     3601 aacctcaaac aggagccgaa gctgctccgg tcggagcgac gcggcgccgc ggaatacgtg
     3661 atcggcgact accacgagat gtactaccgc gtccatcgcc tggagttccg cacccggatc
     3721 acggacgcca ccaacggcca gttccacatc ctgagcctgg tagccggaga ccgcatccag
     3781 atccgatccc tggagcatcc cgagcgctcg tatgagatcg cctactccga gagcgtcatc
     3841 gtccccgcct cggtcggcgc gtacgaggtc gtgaatcgag gccgcggcgc gtgcaaggtc
     3901 accaaggcct tcctccggta gggacggcgc gacggatatg acggcggtgc ctccgcgggg
     3961 cctcgttgcc gccgacatgg gcggctcctt tctgaaattc gcggtggtgg tgtcggagga
     4021 gcccgagcac gcgacggcgg acaggacttt cggccgtgtc cccatgagcg acaccgagga
     4081 gcgctcggtt gccgacgtcc tggcggatgc cgtgggccag gcgctgcgga ccgcggaccg
     4141 tctgggcatc ccggtgagcg ggatcggcgt ctgctctccg gggccgttcg actggcatcg
     4201 cgccgtgagc ctcatgaagc acaagttcct gtccatccag ggacaggagc tctggccgct
     4261 ggtccgcgcg cggctgccgc gcgttgcggc gatcgcgatg cgcctggtcc cggactccta
     4321 cgcattcgcg gccggggagc gggatcatgg ggcggcccgg gactactccc ggtgcctcta
     4381 catcactctc gggaccggca tcgggtccgc cctttccgag aatggccgca tcttcctcca
     4441 cctgccggac aagtctcccg atggatcgat ctggtgcgcc ccgtatcgcg acggcatcac
     4501 ggaggactac gtctccaaga aggccatcct gagcgggtat gccggcgcga gggcgtccgc
     4561 cgccccgagg gaccgcgcgc ccgccgcccc cccaggggac cgcgcgcccg ggctggacgt
     4621 cgccgacatc gccgcgcgag cccgagcggg agaagaggcg gcgctcgacg tgtttggacg
     4681 gttcggccgg cacctgggcg ccgccctgcg agcatacgct tcgggattcg atccccagtg
     4741 catcgtgatt ggcggcgggc tggctccctg ctttccgctg ttcgagtccg agctgaagcg
     4801 ggagacccgg gagatgccgg gactctcggc catcgccgtc tccaccttgg gagagatcgg
     4861 gccgcttctg gggatcgccg ctacctacga ggaagggtac cgcgaaccct gagcgcgtcg
     4921 gcgcgccttg cggggcgcgg ggcccgtgga ctcccgaacc acgaggtccg gctcgatgga
     4981 gatgtgacgc cggcgcttct gccggccctg ctgctcgatc cgcgagatca ggatggttgc
     5041 cgcggtcctc ccgatggcat gcgtgggctg cttcacggtg gtgagcggga cgcccaggaa
     5101 cgaggagaac agcacgtcgt cgaatccgac cagggagatg tccgcgggga tgcgaagccc
     5161 gaactcgcgg atggccttct ccgcgccaaa ggccatcacg tcgttgatgg cgaagatggc
     5221 cgtgggcggg tagcggagct ggagcagctg ccgggtcccc tcgtagccgt ccacctcgtt
     5281 cccgatcttc ccctggtctc cccgatcgcc tttgcggcgc gccacgtact cctcccggaa
     5341 atcgatatgg gagtcctcca gcgccttgcg gtagccggcc aggcgattgg gttcgccgga
     5401 tgccggctcc acgcacccaa tccggttgtg gcccagctcc accaggtgtc gaacggcctt
     5461 gtacgcgccc ttgactccgt ccgtgaggat gaagtccgtc ttgacccgct tgaggttgcc
     5521 gtccacgaac acgatgggca cgccgctctc tttgagctcc tggaacagca ccgtattgtc
     5581 cggaccggac tggacgggtc caacgatgat ccccgccgcc ttgcgtcgcg tcaggacgtc
     5641 cagatagatg gcctctcgct cgatgctgcc ggccgagttg cagaggatcg tggtgtagcc
     5701 gtggctgaag gcggtctcct cgatgccttc cacgatcgac ggatagaagg ggtagatgat
     5761 gtcccggacg atgatcccga tgagcttcgt cttgttgagc cccagctccc gcgccaggag
     5821 gttggggcgg tagtggaggc gcttcgccgc gaccaggacg gccgcccgcg ccttctcgct
     5881 gacgcgaggg ttgttgttga gcgcgtggga gacgaccgtc ggggagtatc cactctcacg
     5941 cgcaacgtcg tagatcgtgg ccatgctgtc cgcgccgcag tatccccagg caaaccgctt
     6001 tgctcggtcg ggacatcttc cccgtgaggc ctatcccgtg tcaagggatc cctgacagcg
     6061 gtgatagcgg tgagatagcg gagatggcgg acggttcctt gacatcccgg atcctttcgt
     6121 cgatagtcta gccaaagcgt cgaaggagac gagtaagcgg ggtgccgtgt cccgagagag
     6181 ggcgctcgat ccgatccatg gcggaagcct gagcggagcg gacggctgga aggcgcctgg
     6241 caagggtccc gttgaaaacc actccctagc tgcggtcgga aaggctggat aggggtccac
     6301 ccggtgggcc gaccaggttc agccgagtat gcgccgccgg tcgcccgccg tgaatctggg
     6361 ctcatcgagg cgcgtgcggc gctcatcgcc atcctgtgcg gagagcggcc gcccgaaaca
     6421 aggtggaacc gcgggaagcc cctgtgcggc ttttcgtcct tgaggatgcg ttcgggaggt
     6481 attcccatgg acgaggcgca ccaggagaag ctgcttcgca ttcgccattc cttctcccat
     6541 gtcatggccg aagcggtgct gcagatgttt ccacgggcca ggctcgccat cggccccgcg
     6601 atcgacgagg ggttctacta cgatttcgag ctgccccgcg cgctggcaac cgaggacctt
     6661 gccgtcctcg aggagaggat gcgggccatc atcgccggcg accacgagtt ccgtcggcat
     6721 tccgtgaccc gggaagaggc gcgccgccgg ttcgcggacc agccctacaa gctggagctg
     6781 atcgccgagc tgccggaagg ggaggacatc tccacttaca cgcaggatac cttcaccgac
     6841 ctgtgccggg ggcctcacgt ctccagcacc cgggagctcc cgccggatgc gttcaagctc
     6901 acgtccgtag ccggcgccta ctggcgcggg gacgagaaga acccgatgct gcagcggatc
     6961 tacggaaccg cctgggagac ccgcaaggaa ctggagacgt ggctcaagag gcgggaggag
     7021 atcgagaagc gggaccaccg ccggctcggc aaggacctgg acctgtactc ggtgcatgag
     7081 gaggccggcg cgggtctcgt ctactggcat ccgaaaggcg cgcgcattcg cttggccatc
     7141 gaggacttct ggcgccggga gcacctgcgc catgggtacg agctggtgta ttccccgcac
     7201 atcggcaagt cctggctttg ggagacctcg ggccacctcg ggttcttcaa ggagagcatg
     7261 tacgcgccgc tcaccatcga cgaggcgagc tactacctga agccgatgaa ctgcccgttc
     7321 cacctcatga tctacaagga ccggcagcga tcctaccgtg agcttcccct tcggtgggcc
     7381 gagctcggga cggtgtatcg gtacgagcga tcgggcgtgt tgcacggtct cctgcgggtg
     7441 cgcgggttca cccaggacga cgcccacatc atctgccccc cggaacggat cgaggatgag
     7501 atcctcgagg tcctgcggtt ctccctcaag atctggaagg cattcgggtt ccaggacatc
     7561 aaggcgtatc tggcaacccg gcccgcgcat gcggtgggca acgacgagag ctggcagaag
     7621 gccatcgcgt ccttgcgccg cgccgcggag gtcgagcagg tgcccctggt gatggacgaa
     7681 gggggagggg cgttctacgg ccccaagatc gacctcaagg tccgggacgc cctggggcgg
     7741 gaatggcaga tgaccacgat ccagttcgac ttcaacgagc cggagctctt cggcatcacc
     7801 tacgtggcgg cggacaacac cccccggcgg ccgttcatgg tgcaccgcgc gctcctgggt
     7861 tcgatggagc gcttcttcgg ggtcctgatc gagcactacg ccggggcgtt ccccggatgg
     7921 ctttcgccgg ttcaggtcat ggccatcccc gtctcagcgg ccttcgtgcc ctatgccgcg
     7981 gaggtggtag gggcgctgaa ggcgcgggat gtccgcgcgg aggtggacgg cggtgacgat
     8041 cgcatgaacg ccaagatccg caacgcccag aaccagaaga tcccctacat gctcgtcctc
     8101 ggggagaagg agcagtcggc gcgaaccgtt tcgctgcgga cacgaagcgg ggaacagcgc
     8161 aaccagatcc cgttggcgga gctcgtgtcc ctgatcgagg cggaagtgcg gccgcccacg
     8221 ctggactgac ggtccgcggc gggaggcggg ggtcggaggg gcggaaggat ggggcgatga
     8281 tcgagatgat cgtcaagcgg gacggccgcg tcgttccgtt cgctcgccgc aagatcaccc
     8341 aggcggttta ccgagccgcg gtggcctgcg gaggccggga ccaggcggag gccgagctgg
     8401 tgaccgatga cgtcctggcg ctcctccaga agcgcaccta ccagggaacc tggccggccg
     8461 tggaggaagt gcaggacctg gtggagaagt gtctgatcga gcgtggccac gcccggaccg
     8521 ccaaggccta catcctctac cgctacgagc acgcgctgaa gcgcgccggg cgggagagcc
     8581 tgacctactc tcgggagaac atcccgtacc gaaagctctg ggaggcgctg tcctgggcca
     8641 cggaccacga ctgcgtgacc ctggcccgcc tcacctccat catccagagc ggcgcgctgc
     8701 cggatctcat ccaggcctcg gaggacttct acgagcagga gctggagagc gccgcggaga
     8761 agatcctggc ccgaagagac gagcttcgga tcgtcatcat cgccgggccg tcttcctcag
     8821 gcaagacgac gaccacgctc aaggtgcgcg acaagctcca ggcagcgggg atgcgcacgc
     8881 tccccatctc cctggacaac tacttcttcg atctgaagat gcatccccgg gtcggggaga
     8941 acgactacga tttcgagaca ccgcaggcgc tggacctggc cctgatcaac cggcacctcc
     9001 acgccctggc atccggagag gccgtggagg tcccgcggta caacttcaag accggccaac
     9061 gggaagggat ctccgggacg ctccggctgg agacggacca ggtgctcctc atcgattcgc
     9121 tccacggcct tttcccggag atgacgagcg ccgtggcgga agagaaaaag ttccggctgt
     9181 acatcgagac gctctcccag atcaagggtc cggacggccg ctacctgcga tgggcggaca
     9241 tccgcatgct ccgccgcgtg gtgcgggaca tgcagttccg cggctactcg ccgcggcaga
     9301 ccatccgcca ctggcacctg gtgcggcgct cggagctccg ctacatcgtg ccggagcttc
     9361 gccgcgccca tgccattgtc aacagcttcc tgccgtacga gcttccgatc atgaaggccc
     9421 gcgtgggcga aagcctgcgg ggactggccg aagagttcgg ccgggatcct gaggcgcagg
     9481 acgcgcagga gagggccgcc cgcgtgatgg cggtcttcga gcaactcccc gccgtgcagg
     9541 acgaggggct ggtgccgggg cggtcgctcc tgcgtgagtt catcggcggc agcacgtatc
     9601 ggtacgagta gcggccggag gtggtcgtgg acgggaagtc gatggtcagt gctctccttg
     9661 ccagtccgct cccctttgcc gtcctgggcg gcatcttcgt cgggatggcc gtgagtcgcg
     9721 ccacgcgtcg gaccgaccgc tcccgggatc ccgcccgggc gcgcacggga aagtgggttg
     9781 ggttctgcgt ctacctgtct ctggccgtgg tcctcggcat cctggccgtg ttcctgccgg
     9841 gcgcccgcaa gatcgcggat atccgcgtgg cgtacgttgc ggcagcggcc gccatcctgt
     9901 ctttcgcggg cacccgcttc cgcctggcgc tcggtattcc tatcgtggtc atcctggttc
     9961 tggccgtgac gaccggcgcc ctcttcctga ggagcatccg cgcattcacc ggggagacca
    10021 ccatcgccac cgtaagggtc atctcggcgg gccaggattc gatgcggctt cagctcctgc
    10081 cccgcgacgg tgagccggcc atggtcacca tgcaaggcga gtacttcgcg cccatcgtca
    10141 aggtggtgat cttcaacgac ctgtgggtct tcctgggcgc ccatacctgg taccggttcg
    10201 aggggctgac ctccttccgg atggtgaaag agggcgacgg ctaccggctt cgccagggaa
    10261 cgacggacta ttaccttccg cgcgccgcgg gcatctcgga gcggctctgg tcactcttcg
    10321 aggcgaacga gcgcagcatc ccgggtgtga agaccgtcca gatcgagatg gacctcaaac
    10381 gcgccgagcc gacggagccc gccgcccagg agctccacag ctacgagatc cgagtccaga
    10441 acgacggtgg tgtggaagta gtgccggtga acggctagga tcccgtgttc tcccgccgct
    10501 tccctcggcc caccgccacc tcggcgaacc ttccccacag gaagacgacc ttgtctccca
    10561 tggagaccac cacgacggcc gcaacgacgt attccaggat ggacaccacg gtggggttgc
    10621 cgatccaggg gacaccgaac acaccggcga tctccgcgag gtagaggacc atcagggcga
    10681 agatcgagac cttcccgagg aacgtggcga gcgggtttgc cttcccctcg cgtagcgcca
    10741 ggaccgccat acccagagcg aagaggacca gacgggccag caccaggatg aagaaccaca
    10801 aggggatgag cccgttgata tagaagagaa tggacatggc gatgatcgtc acgtagtcgc
    10861 tgctggagtc caggtacctg ccgacaaacg tgatctcccc ccggcggcgc gccaccatgc
    10921 cgtccacgaa gtcggtcgcg aacacgacgc agacgaacgg gagcgtgacc ggcaggaccc
    10981 gatacgcggg ggcctgcatc accatgtaca ggatcgtcgg gatggagctg agccggccga
    11041 acgagagggt gttggcgagg ttgacgcgct ccagccgcag gccggccggt tccgttcgga
    11101 agtcctccca tcgcgcgagc aggacgcccg tgagcagggc gtgatacgcc agcgcgacgg
    11161 cagcgaacag cgcggccctg ggaagctcca taccgtagcc gaaggtcagg gcggcgatga
    11221 tcgcctcctg cagaaccagg aaggcgatgc cgtgggccag gacacttcgg cggattccgg
    11281 ggactgggcg catcaggcgg agcctaccac ggcgcccgcc cggccgaaaa ggccgcctct
    11341 tccccattga cagccgggga atcggcgcgc cgacggaatc cggccgatgc gttgacgaaa
    11401 gcgcgtcgga cactatgatg gcgcccatgg ttcccatggt cctgacggtc ctgcgaatgg
    11461 tcctggcccc cgtgttcttc ggcttctacg ctcttgcctc ggcggggtct ccgtcattcg
    11521 tgatcggagt gtggctggtg tttgccgcga tcgagatctc cgatgtcctg gacgggcacc
    11581 tcgctcggag cctgcggcag gaaaccgagg ccggcaaggt cctggacccg ttggccgact
    11641 cggtaagcag gctcacctat tttctttgct tgacgggaac tggcatcatg cccctttggg
    11701 tcctcctcat tctgatctat cgggacctcg gcgtagccta catacgggtc atggtttccc
    11761 gctccagcgt cctgctgccg gcgcggctca gcggcaagct caaggcgtgg gtatacgcct
    11821 tcactggcgg cgtggggatc gcggttttct cggtgcgccg tctcgggtgg cttccgtcct
    11881 tgcagaacgt tgccgggacg atctccctcg ggctatgcgt gcttgcggcc ctgatcgcgg
    11941 tatggtctct cgccgactac gcggcttttt tcttgcggaa tttccgtaga actagttgac
    12001 acgttctggt gcacctgtat atattctgat tcgcctcact gtggcagtgt actctcagta
    12061 ctcaagttga ggggggaagt accgtctctg gggcaacgtg cgtgaggggc agccgggaaa
    12121 cggcgggtcc gagcgcactg gatctttgac agtcaaggga aggggagaat gaggtgagaa
    12181 cgggctccgg tccgctccaa ggggagtgga cttagatact gatagaaaac ctgtccgcaa
    12241 ggacaggcct tatatcacgg agagtttgat cctggctcag aacgaacgct ggcggcgcgt
    12301 tttaagcatg caagtcggac ggcaagttgg gtagcaatac ctgacctaga gtggcgaacg
    12361 ggtgagtaac acgtaggtga tctgcctctg gatgggggat agcccgtgga aacacggggt
    12421 aataccgcat gaggccttac gggtaaagcc gtgaggtgaa aggtgcaaat gcatcgtctg
    12481 gggatgagcc tgcggtccat tagttagttg gcggggtaac ggcccaccaa gacgacgatg
    12541 gatagccggc ctgagagggt gtacggccac actggaactg agacacggtc cagactccta
    12601 cgggaggcag cagct
//
LOCUS       DASRZS010000196        12813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2411907, whole genome shotgun sequence.
ACCESSION   DASRZS010000196 DASRZS010000000
VERSION     DASRZS010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 12813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12813
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2411907"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFH83_04840"
     CDS             complement(<1..530)
                     /locus_tag="VFH83_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485721.1"
                     /translation="MLSLVLKVSKGVPALPPAIREEKGLIVQRINHIDSDRDGAPLVA
                     WLLDTDFLTQHHKALSAGGVADGSVLAWNPDGETDVSRQIPGGLLYDEVASLDRPTLF
                     LRTLRNLYRRLHLEREVAEKDRLLKARESQNSDLLQIGIALSAERNNDKLLGYILKQL
                     RLMTAADSGTLYLLER"
     gene            complement(569..2341)
                     /locus_tag="VFH83_04845"
     CDS             complement(569..2341)
                     /locus_tag="VFH83_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485722.1"
                     /translation="MTRVPRKAVVLVIALLLPAARLLAQPVPDETLDQLYGRAREAIT
                     RQAYESAVGILKEAKTQYPASPKPSVALGDLYYDQELYALALTEYRDAEKKGADDFQT
                     LTQISRSYGKLNREPQSIDYLKRILKRFPESTDTVDDLGWMYFKTHQLRQGEEVLLDG
                     INRLGLSRSLAMTLGTVYSGMNQYDRSRQYYQKAIDEALGAQDRTFAAIAYYNLALLE
                     HNYYQYNSALRSTEDSIAMEDRPSGHLARGELFESRMEFDSAWQEYQQAYAKDDTPLS
                     KVNMAILLRKFGRLELASRYAEDVLSSRDLSWMVYYGTDTTRHVKDMHELLQQIDQGL
                     AQVQLHRPTAGPLDRLKALWAAVRYAVEAWQHRQRFRLLSVSVGDDYLAQGSNEDAWW
                     EYYRGNEGYPEVALKYLELARSSEVQRSPHAAVFYQMEEGRLRRSVPLLRDALPGFDP
                     YWEKGAIAEDLTALIPLLNGPGNRSTRRDDIVRLYQINPGALPQAGLGLPLHVEFTGN
                     GWKARERAIILRLISRSGSEIDASAPYSLRFTRGPGGEVRWIVMDPSKGAAVREGTSG
                     AGGSLRARCSRLVQSVLDELYAVH"
     gene            complement(2338..4476)
                     /locus_tag="VFH83_04850"
     CDS             complement(2338..4476)
                     /locus_tag="VFH83_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HET6485723.1"
                     /translation="MPRKSELLRGGLLLLLRNLLLYFLLVNAYFQLRETLDLTFQTVV
                     FLLAILTALLLAASRLRFLPSLVLAAAVPLGLRALFFLVFHLQRVIASGPATDFVFLY
                     FDKDFFPALVVWGVAWLLNFLARRHPPFLLVEALIDAALLLAVFWSQAGYKLTLYPHP
                     SIFAWALAVFIVIQIFALLVSGEARGRGPARVRAARARSVLSFAWIVVPLLLVFLLFL
                     LDRYGEGAVKAGGGLMKPTLFRFDFAPYIRLESEIRTSDETVLLFRTEGKAERFLLRR
                     FVLSGYEKNQGFFMERGKGMDEYIPIVPDAPQTYPDPGYRGRLSVSQEYYFITLDPSS
                     LIALNYPVSVTPLQNWKSSSFLRVYRTQSRAIRSLQPEHLVVAQPAMPADQLGYYTSA
                     SADKQVADLAREITAGMVGYDAKVTAIERYLRTNYWYSLKPGIAEDGDQLHHFLFVSK
                     KGYCSYFAFAMALMCRSLGIPARVAVGFYVDPQSEVLNFYQVRAFQAHAWVEVYYGNL
                     GWLEFDPTSDRLAPGEEFTNPPGPDRERMAKLIAEILNNQTNIEEWQPTRSAAARSLA
                     GIADTIARLAAVVARLWYFTLPILYVLYLVGSKLLPRLPGLASRDPRRRVKARYRLEL
                     VRLAGVGLARRAGESPLEHARRVEAATGACLEPLTRSYLQAAFGPSFEAPQESRARQA
                     AMSFAGSYRQRIGAPLRVTGLLNPVVGRVP"
     gene            complement(4460..5776)
                     /locus_tag="VFH83_04855"
     CDS             complement(4460..5776)
                     /locus_tag="VFH83_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6485724.1"
                     /translation="MNLLRRPWHSIRIAVPLTPRGAVVTGVAAALLVEGILRADLAGL
                     FWGASFVVLPVYAVAAGHLLRFRLRRRSRSGKDSLSLELPGDGLDRGKPAEARLSIRL
                     PRSFPPGFSVRALLPLRWQQRRLTGVEAALSTGENHRLIPFTAAHRGAFQSDGIRLQV
                     SDLLQLTRADVLVARHSTVTVFPGVTAAQRARRTAEESEEAAPRARSRRRTEELLEAR
                     KYYPGDDPRRINWKVYAHMGELFLRIGEETPPPESRVLFVLDASANPLVPDSVAADYL
                     DSLVDAYSSAVVDLLAQDVDLLAMVPGVLKCASFTRESQATLLGALAGVWWASSSGPF
                     ELPSRRGLHVAVYSSPGSPGLSRIIEAARARGYTTSLFLKGLPPEPPARRFSLSRLLL
                     VPSAPRRDPPADGATRGQREALVRALTADLTSYQGPPWMVRYAAEV"
     gene            complement(5789..6778)
                     /locus_tag="VFH83_04860"
     CDS             complement(5789..6778)
                     /locus_tag="VFH83_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6485725.1"
                     /translation="MGDSSAPSRPPLSFEASHGLARRLVEGMAAVIRGKREVLEHLVT
                     AILAGGHVLIEDVPGLGKTTLAKTIARLVSRTRRGSPVVFRRIQFTPDLLPYDITGVD
                     VFDPASKSFVFTPGPVFANVLLADEINRTTPKVQSALLEVMAEGQVTVGNKTYPMDPF
                     FFVIATQNPVEMEGVYPLPLAQIDRFLMKVHVGYPDLEVEVGIVRDDPVSTVMPSVKP
                     VCGRDEILAARDAVARVFCDEKLLRVAVALSAATRKHPAVGLGASPRGSLMLVRAARA
                     YALVRGREYVIDQDLIDLAPFVITHRLRLKDPRADGADLVREMIMAELARTSY"
     gene            complement(6798..7256)
                     /locus_tag="VFH83_04865"
     CDS             complement(6798..7256)
                     /locus_tag="VFH83_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485726.1"
                     /translation="MSEAVLEKVSVPLSGDPEILSLIAEVREVFENHFDRTWFSLVLD
                     GMPIDLRTVRDIRSLVSLTEIFPGEEQHLYQGVLELEKFCNALKRLLLPVLKERLGIS
                     WLKPKWRVRDPQQYILRKFVAFTFPYNLERLRRMTQRLKALLLSHYPSLR"
     gene            complement(7338..8099)
                     /locus_tag="VFH83_04870"
     CDS             complement(7338..8099)
                     /locus_tag="VFH83_04870"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HET6485727.1"
                     /translation="MILKLISCEVFYREACLCVATSPHRVDLEFTEKNAHERSDYLRS
                     VLQARIEAAEAGETPYDAILLGFGLCGNGLLGIRASKTRLVLPRAHDCCTIFLGSRTA
                     FKRHFEDNPSLPFSSVGYMERGGSWVHDADTVKVPGLDKSLEDYIALYGEENARYIME
                     TLTTSTKTAVGDTADNRVVFIDVPELSHLGYEEKCRKEAEAIGKQFVKLPGDLRLMRN
                     LVHGSWDPEEFLVVQPGQKIGGVYDWDAVVRAEDA"
     gene            8205..9044
                     /locus_tag="VFH83_04875"
     CDS             8205..9044
                     /locus_tag="VFH83_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6485728.1"
                     /translation="MANVMAGRTCLVTGGTSGVGRAIASGLARLGADVLLVSSSVERG
                     SRAAADLRRETGGTVESLTADLSRMDSVRALSVAFRQRRTVLHVLSLNAAVLPMKRRL
                     TVDGLDSVFATNYLGQFLLTRLLMDLLRKSAPARVITVSGTPGSLRRGDVRYDDISLE
                     IGWNPLRATLQAAFAKVVFTYELARRLSGTGVTANTFHPGLVKSNLPRSLPWFLRGPA
                     ALAELLMSTECPTGVYLASAPEVKEVTGAFFVGRSARRFDPPAAGPDAGKRLWALSEK
                     LAG"
     gene            complement(9054..11138)
                     /locus_tag="VFH83_04880"
     CDS             complement(9054..11138)
                     /locus_tag="VFH83_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485729.1"
                     /translation="MRRRTVTLAAAALLAAGLLAPFVSGAQAASPAAAEKTVTIPISS
                     MVLFTSGVTYFQHDGVVDGNAQMELTFSASQINDLLKSLVVRDLDGGSITSVTYSSRD
                     PITRTLKTFAVDLTSDPTLSNLLVQTRGEAVEVTLTNASKVTGTIISVETRQEQTSPK
                     GVVAGDFVNISTAAGVRSIALQDVEGIRFLRKEIQDDLAQALLVLSASHGVEKKKLVL
                     HFSGAGKRRVRIGYILESPVWKTSYRLVLAETGQHLLQGWALVENTTDTDWSQVGLKL
                     VSGRPITFTMDLYQPLYIQRPEVQLELYQSLVPKTNEMAMDQEAPMEEATAAGAPAPA
                     SAPAAKSMAQPAGPRAALGFAAPAPESFNPAQGVNAAATGSAVGELFQYVIDKPVTLP
                     RQQSAMLPILNQLVDGARYSLYNPATEPTHPLNAVRLKNASALHLMQGPITVFDGGSY
                     AGDAQITDLPPGAEQLITYALDLDTEIQSTQGASPTTLISVRIGKGVFYFSNRLQRER
                     DYSIANRGAKDRTVLIEQPIASDWDLTRPDKPLERTRDNYRFAVPVAAGKSGSLAVVE
                     TRLVSQSIALSSMGSDQIAVYLQAPVVSQAVKDALQTLVSLQQKLADTVSQRQRQEAR
                     VNEIANDQSRIRQNMDRLDKTSDLYKQYVKTLTDQETELSSLRTAIATLRDQEASQRS
                     DIASYIQSLDVS"
     gene            complement(11140..11748)
                     /locus_tag="VFH83_04885"
     CDS             complement(11140..11748)
                     /locus_tag="VFH83_04885"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET6485730.1"
                     /translation="MGIRLRYNAPVILTFTIIAAVVLLIDQLTGGSLIRTYFTIYPGF
                     NARSLLSWFTLFSYVIGHQSWNHLISNFSFILLIGPILEEKYGSVPLLLMMLVTAVST
                     GILNILLVRTGLLGASGIVFMLILLSSFTNIRQGDIPITFILIVALYLVREFVNALTP
                     STISQLAHIAGGVIGGLFGFVFAARESTARALPSTTPGEKED"
     gene            complement(11777..>12813)
                     /locus_tag="VFH83_04890"
     CDS             complement(11777..>12813)
                     /locus_tag="VFH83_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HET6485731.1"
                     /translation="EGDESGHEGDEIVHVLESAVPAGPVRCVVDAARRLDYMTQHTGQ
                     HILSQALVRAGDLPTVSVHFGEQTTTIELAVPEVRAEVLDAAEELANGAIRRNLGVRV
                     HEIDRSELSKYPLRRTPPKAQRLRIVEVDGFDWAACGGVHVRNAGELLLIKVVGQERI
                     RGRVRIHAVIGARALADYRRKTALVQALSRALTCGEENMLSRVEELSARGKEMAQEIR
                     RLTTRLAAADADQAASSARAVGGAAIVRRGLQGAGADYLKLFAEGVIARPGRVVIVTD
                     RTAEAFQWLVAQSVGDRLDLGGLLPPLYAIGGCKGGGKGARVQGRGTRPDAAEAFAQA
                     VEEEIARRLS"
BASE COUNT         2116 a         4195 c         4453 g         2049 t
ORIGIN      
        1 tcgcgctcca gcaggtagag ggtgcccgag tccgcggcgg tcatcagtcg aagctgcttc
       61 aggatatagc ccagaagctt gtcgttgttg cgctccgcgg aaagcgcaat gccgatctgc
      121 aggagatcgg agttctgaga ctcccgcgcc ttcagcagtc ggtccttctc cgccacctcc
      181 cgctccaggt ggagccgacg gtagaggttt cggagcgttc gcaggaagag cgtcggccgg
      241 tccagggacg cgacctcgtc gtagaggagg ccgcccggga tctgccggga gacgtccgtc
      301 tcgccgtccg ggttccacgc caggacggag ccgtcggcca ctccgcccgc cgacagcgcc
      361 ttgtgatgct gcgtcaggaa gtcggtgtcc aggagccacg ccaccagggg ggccccgtcc
      421 cggtcggagt cgatgtggtt gatgcgctgt acgatcagac ctttttcttc acgaatcgcg
      481 ggcgggagcg cgggaacgcc tttcgatact ttcagaacga gagatagcac caggactcct
      541 ttgggcctac cattatccta ctgatagttt agtgaaccgc gtacaactca tcaagaacgg
      601 attgcaccag acgggagcat ctggcgcgga ggctgccgcc ggcccctgac gtgccttcgc
      661 ggaccgcggc gcctttcgag ggatccatca cgatccaccg gacctccccg ccggggccgc
      721 gcgtgaaccg caggctgtat ggggccgaag cgtcgatctc cgagccggac cgggaaatca
      781 gccggaggat gatggcgcgc tcccgcgcct tccagccgtt gccggtgaac tcgacgtgga
      841 gcggcagccc caggccggcc tggggaaggg cgccggggtt gatctggtag agtcggacga
      901 tgtcgtcccg ccgagtcgag cggttgccag gaccgttgag aagcgggatg agcgcggtca
      961 ggtcctccgc gatggcccct ttttcccagt agggatcgaa cccgggaagg gcgtccctca
     1021 ggagcgggac ggaccggcga agccggccct cttccatctg gtagaacacc gccgcgtgag
     1081 gagagcgctg gacctccgag ctccgcgcca gctccaggta cttgagcgcc acctcggggt
     1141 agccctcgtt gccgcggtag tactcccacc aggcgtcctc gttggagccc tgggccaggt
     1201 agtcgtcccc cacgctcacg gagaggagtc ggaagcgctg gcggtgctgc caggcttcga
     1261 cggcatagcg gaccgcggcc caaagcgcct tgagccgatc caggggaccc gccgtggggc
     1321 gatggagctg gacctgggcc agcccctggt cgatctgctg caggagctcg tgcatgtcct
     1381 tcacgtgtcg cgtcgtgtcg gtgccgtagt agaccatcca ggagaggtcc cgggaggaaa
     1441 ggacgtcctc cgcgtagcga ctggccagct ccaatcgtcc gaacttcctg agcaggattg
     1501 ccatgttcac cttggagagc ggcgtgtcgt ccttggcgta cgcctgctgg tattcctgcc
     1561 aggcggagtc gaactccatg cggctctcga aaagctcgcc gcgcgccagg tgcccggacg
     1621 gacggtcctc catggcgatg gagtcctcgg tggaccgaag ggccgagttg tactggtagt
     1681 agttgtgctc caggagcgcc aggttgtagt acgcgatggc cgcaaaggtc cgatcctgcg
     1741 cgcccagcgc ctcgtcgatc gccttctggt agtactgacg ggatcggtcg tactggttca
     1801 tgccggagta gacggtgccc agggtcatgg ccaggctgcg gctcagcccc agccggttga
     1861 tcccgtccag gagcacctcc tccccctggc ggagctgatg ggtcttgaag tacatccatc
     1921 ccaggtcatc caccgtgtcc gtgctctcgg ggaaccgctt gaggatgcgc ttcagatagt
     1981 cgatggactg gggttcccgg ttgagcttgc cgtagctacg ggagatctgc gtcagggtct
     2041 ggaagtcatc cgcgcccttc ttctcggcat cgcggtactc cgtcagcgcc agcgcgtaga
     2101 gctcctggtc gtagtagagg tcgccgaggg ccacgctcgg tttcggggag gcgggatact
     2161 gggtcttcgc ctccttgagg atgccgacgg cggactcgta ggcctgcctg gtgatcgcct
     2221 cccgcgcccg cccgtagagc tggtcgagcg tctcgtcggg aacgggttgc gccaggagcc
     2281 gggccgctgg caggagaagg gcgatgacga ggacgacggc cttccggggc acccgcgtca
     2341 cggaactctc cctaccacgg ggttcagaag ccctgtcacc cgaaggggcg ccccgatgcg
     2401 ttgtcggtag gagccggcaa aggacatcgc cgcctggcgc gcgcggctct cctggggcgc
     2461 ctcgaagctc gggccgaacg cggcctgaag gtatgaccgg gtgagcggct ccaggcaggc
     2521 acccgtcgcg gcctccaccc gccgcgcgtg ctccagcggc gactccccgg cgcgccgcgc
     2581 cagcccgacg ccggccagcc ggaccagctc cagacggtag cgtgccttca cccggcgtcg
     2641 ggggtcgcgg ctggcaaggc caggcaacct gggcaggagc ttggagccga cgaggtacag
     2701 cacatagagg atgggcaggg tgaagtacca caaccgggcc accacggcgg caagcctggc
     2761 gatggtatcc gcgatgcccg ccagggagcg cgcggccgcc gatcgcgtgg gctgccactc
     2821 ctcgatgttg gtctggttgt tcaggatctc cgcgatcagc ttggccatcc gctcgcggtc
     2881 gggccccggc gggttggtga actcctctcc gggcgccagc cgatccgagg tgggatcgaa
     2941 ctccagccat cccaggttgc cgtagtagac ctccacccac gcgtgcgcct ggaaggcgcg
     3001 cacctggtag aagttgagca cctcggactg gggatccacg tagaagccca ccgccacccg
     3061 ggccggtatg cccaggctgc ggcacatcag cgccatcgcg aacgcgaagt aggagcagta
     3121 acccttcttc gagacgaaga ggaagtggtg tagctggtcg ccgtcctccg cgatgcctgg
     3181 cttgagggag taccagtagt tggtccgaag gtaccgctcg atggccgtga ccttggcgtc
     3241 gtagccgacc atgccggcgg tgatctctcg cgccaggtcg gccacctgct tgtccgcgct
     3301 cgccgaggtg tagtagccga gctggtccgc gggcatggcc ggctgcgcca cgacaaggtg
     3361 ctccggctga agggagcgga tcgcccgaga ctgggtccgg tagacgcgga ggaaggagga
     3421 agacttccag ttctgaagcg gggtgacgct caccggatag ttcaaagcga tgagcgatga
     3481 cgggtccagc gtgatgaagt agtactcctg gctcacggac aggcggcccc gatagccggg
     3541 gtccgggtag gtctgcggcg cgtccggcac gatagggatg tactcgtcca tccctttgcc
     3601 gcgctccatg aagaagccct ggttcttctc gtagccggag agcacgaacc gacgcagaag
     3661 gaagcgctcc gccttgccct cggtccggaa gaggagcacg gtctcgtcgc tggtgcggat
     3721 ctcgctctcc aggcggatgt acggggcgaa gtcgaaccgg aacagcgtgg gcttcatcag
     3781 gccgccgccc gccttcacgg cgccctcccc gtagcggtcc aggaggaaga ggagaaacac
     3841 gagcaggagc gggaccacga tccacgcgaa gctgagcacg gatcgcgcgc gggccgctcg
     3901 cacccgggcc ggcccccggc cccgcgcctc gccggagacc aggagcgcga agatctggat
     3961 gacgatgaag accgcgagcg cccaagcgaa gatggacggg tgagggtaga gcgtgagctt
     4021 gtagcccgcc tgggaccaga acacggccag gagcagcgcc gcgtcgatca gcgcctccac
     4081 gaggaggaac ggcgggtggc ggcgggccag gaagttgagg agccacgcca cgccccagac
     4141 cacaagcgcc gggaagaagt ccttgtcgaa gtagaggaag acgaagtcgg tggctggccc
     4201 ggaagctatg acacgctgca gatggaacac caggaagaag agcgcgcgca ggccaagcgg
     4261 gacggccgcg gccaggacca gggacggaag gaagcggagc cggctcgccg ccaggaggag
     4321 cgcggtcagg atcgccagga ggaagaccac ggtctggaag gtcaggtcca gggtctcgcg
     4381 gagctggaag taggcgttca caaggaggaa gtagaggaga agattccgaa gaaggaggag
     4441 caggcctcct cgcagaagct cagacttccg cggcataccg caccatccag ggaggcccct
     4501 ggtaactcgt caagtccgcc gtcagggcgc gcaccagcgc ctcccgttga ccgcgggtgg
     4561 cgccgtcggc aggagggtcg cggcgcggcg ctgacggcac cagcagaagc cgtgacaggg
     4621 agaatcggcg cgcgggcggc tccggtggca aacccttcag gaacaggctc gtcgtgtagc
     4681 cgcgggctcg cgccgcctcg atgatccgcg acaggccagg agagcccgga gaggagtaga
     4741 ccgccacgtg cagtccccga cgggagggaa gctcgaaggg accgctggag gaggcccacc
     4801 agacgccggc cagagcaccc aggagggtcg cctgggactc tcgagtgaaa gaagcgcact
     4861 tgaggacgcc gggcaccatg gccagcaggt ccacgtcctg ggccaggagg tccaccaccg
     4921 cggaggagta ggcgtccacc aggctgtcca ggtagtcggc ggcaacggag tccggcacca
     4981 ggggattcgc gctcgcgtcc agcacgaaca ggacccgcga ctccggcggc ggagtctcct
     5041 cgccgatgcg gaggaagagc tcgcccatgt gggcgtacac cttccagttg atccggcgcg
     5101 gatcgtcccc cgggtagtac ttgcgggctt cgagcagctc ctccgtccgg cgccggctcc
     5161 tggctcgggg cgcggcctcc tcgctctcct ccgcggtccg gcgggcccgc tgcgcggcgg
     5221 tcacgccagg gaacaccgtg accgtggagt ggcgggccac caggacgtcg gcgcgcgtaa
     5281 gctggaggag gtcgctcacc tggagacgga tcccgtcgct ctggaacgcg ccgcgatgcg
     5341 ccgccgtgaa agggatcagg cggtggtttt cccccgtaga gagcgccgcc tccacgccgg
     5401 tcaggcgcct ctgctgccac cgcagaggca ggagcgcgcg gaccgagaag cctggcggga
     5461 aagacctggg gagacggatg gacaggcggg cctccgccgg cttgccccgg tccaggccgt
     5521 ctccaggtag ctccagggac agggagtcct tgccggaccg ggaccgccgg cggaggcgga
     5581 accgcaggag gtggccggcg gcaacggcgt agacgggaag caccacgaaa ctggcgcccc
     5641 agaagaggcc ggccagatcg gcgcggagga tgccttctac cagaagggcc gccgccaccc
     5701 cggtcacgac cgctcctcgg ggggtgaggg gaaccgcgat ccgtatcgag tgccagggtc
     5761 gtcgcaggag gttcatcgag gcatcggatc agtagctggt acgcgcgagc tccgccatga
     5821 tcatctcccg gaccaggtct gccccgtcgg cgcgcgggtc cttgagccgc aggcgatgcg
     5881 tgatcacgaa aggcgccagg tcaatgaggt cctggtcgat cacgtactcc cgaccgcgca
     5941 ccagcgcgta agcccgggcc gcgcgcacca gcatcaggct gccgcgggga gaggcgccca
     6001 ggcccaccgc cgggtgcttt cgggtggcgg cggagagcgc cacggccacc cgcaggagct
     6061 tctcgtcgca gaacacgcgg gccaccgcgt cacgcgccgc caggatctcg tccctgccgc
     6121 acaccggctt taccgacggc atgaccgtgc tcaccggatc gtcccggacg atgcccacct
     6181 ccacctcgag gtccggatag cctacgtgca ccttcatcag gaaccggtcg atctgcgcca
     6241 acgggagcgg gtagacaccc tccatctcca ccgggttctg cgtggcaatc acgaagaaga
     6301 acgggtccat ggggtaggtc ttgttgccga ccgtgacctg accttccgcc atgacctcca
     6361 ggagcgcgga ctgcaccttc ggggtggtgc ggttgatctc gtcggccagg aggacgttgg
     6421 cgaagaccgg gcccggcgtg aaaacgaagc tcttgctggc gggatcgaac acgtccacac
     6481 cggtaatgtc gtagggaagg aggtccggag tgaactggat tcggcggaat accacgggac
     6541 tgccgcgtcg ggtccgggag accagccggg caatggtctt ggccagggtg gtcttcccca
     6601 gaccgggaac gtcttcgatc agcacgtggc cgcccgccag gatggccgtg accaggtgct
     6661 ccaggacttc ccgcttgcct cggatgaccg cggccatccc ctccaccaga cgccgtgcca
     6721 ggccgtggga cgcctcgaag ctcagggggg gccgcgacgg ggcggaagag tcacccatcg
     6781 tctgaaactg tagcacctca gcggagggat ggatagtgcg agagcagaag cgccttgagc
     6841 cgctgcgtca tgcggcgcag ccgttccagg ttgtacggga acgtgaaggc gacgaacttc
     6901 ctcaatatgt actgctgcgg gtcgcgcacc cgccatttcg gcttgagcca ggagatcccc
     6961 agccgctcct tgagcacggg caggaggagt cgcttcagcg cgttgcagaa cttctccagc
     7021 tccaggacgc cctggtacag gtgctgctct tctccgggaa agatctcggt cagggaaacc
     7081 agggaccgga tgtcccgcac ggtccgcagg tcgatcggca tgccgtccag gaccagggag
     7141 aaccaggtcc ggtcgaagtg gttctcgaac acttcccgca cctcggcaat gaggctgagg
     7201 atctcggggt cacccgagag tggtacgctg accttctcca gtacggcttc cgacacccga
     7261 catcactccg tcctctacca atgtcggaac cggaagggaa tggcttgatg agcgtgatgc
     7321 gggtcggggg cctgcctcta ggcgtcctcc gcccggacca cggcgtccca atcgtagact
     7381 ccgccgatct tctgtcccgg ctggactacc aggaactcct cggggtccca cgagccgtgc
     7441 accaggttgc gcatcagccg aagatccccc ggcagcttga cgaactgctt gccgatggcc
     7501 tcggcctcct tgcggcactt ctcctcgtag cccaggtgcg acagctccgg cacgtcgatg
     7561 aagacgacgc ggttgtccgc cgtgtctccg acggccgtct tcgtggacgt ggtgagcgtc
     7621 tccatgatgt agcgcgcgtt ctcctccccg tagagggcga tgtagtcttc cagactcttg
     7681 tcgagccccg gcaccttgac cgtgtccgcg tcatggaccc acgagccgcc gcgctccatg
     7741 tatcccacgg aggagaacgg caggctgggg ttatcctcga agtgtcgctt gaacgccgtc
     7801 cgggacccca ggaagatggt gcagcagtcg tgcgccctgg gcagcaccag gcgcgtcttg
     7861 ctcgcccgga tgcccaacag cccgttcccg cacaggccga agcccaggag gatcgcatcg
     7921 tagggagtct cgccggcctc cgccgcctcg atccgggcct ggaggacgga acgcaggtag
     7981 tcgctgcgct cgtgcgcgtt cttctccgtg aactccaggt ccacccggtg gggcgaggtg
     8041 gcaacgcaga gacaggcctc ccgatagaag acctcgcagg agatgagctt cagtatcatg
     8101 gcggacggtg taccacgccc gggccgcgag tgtcacccgg tgggtgtcgc gcgggcgccg
     8161 cccgtttgac gagcggaaga gccctcccgt acctttggac gcatatggcg aatgtcatgg
     8221 cgggcagaac ctgcctggtg accggcggaa cctcgggcgt gggaagggcg attgcgagcg
     8281 ggctggcccg gctcggcgcc gacgtgctcc tggtgagcag cagcgtcgag cggggatcac
     8341 gggccgccgc ggacctccga cgggagaccg gcggcacggt ggaaagcctg accgcggacc
     8401 tctctcggat ggactccgtc cgagccctct ccgtcgcgtt ccgacagcgg cgcacggtgc
     8461 ttcacgtgct ttccctgaac gccgcggtcc tccccatgaa gcggcggctc accgtggacg
     8521 gcctggacag cgtctttgcc accaactacc tgggtcagtt cctgctgact cggctgctga
     8581 tggaccttct ccgcaagagc gcgccggcgc gggtcatcac ggtgtccggc acccccgggt
     8641 cccttcggcg cggggacgtc cgctacgacg acatcagcct ggagatcgga tggaacccgc
     8701 tgcgggctac gctgcaggcc gcgttcgcca aggtggtctt cacctacgag ctggcccgcc
     8761 ggctttcggg gaccggggtg acggccaaca cgttccaccc cggactcgtg aaatccaatc
     8821 tgccgcggag cctcccctgg ttcctgcgcg gtccggccgc gctcgcggag ctcctgatgt
     8881 ccacggagtg ccctaccggc gtctacctgg cctccgcgcc ggaggtgaag gaggtcacgg
     8941 gcgccttctt cgtggggaga agcgcccgtc ggttcgaccc gccggccgcc ggcccggatg
     9001 ccggcaagcg cctctgggcg ctgagcgaga agctcgccgg gtgagggtga ggctcagctc
     9061 acgtccagag actgtatgta gctcgcgatg tccgatcgct gggaagcctc ctggtcccgg
     9121 agcgtggcga tggccgtccg aaggctcgag agctccgtct cctggtccgt gagcgtcttg
     9181 acgtactgct tgtagaggtc cgaggtcttg tccagccgat ccatgttctg ccggatgcgg
     9241 ctctggtcat tcgcgatctc gtttacccgg gcctcttgcc tctgacgttg ggagacggta
     9301 tccgcgagct tctgctgcag ggagaccagg gtctgcaggg cgtccttcac ggcctggctc
     9361 accacgggcg cctggagata gacggcgatc tggtccgacc ccatgctgga gagggcgatg
     9421 ctctggctca ccagccgcgt ctccaccacg gcaagagagc cgctcttccc ggcagccacc
     9481 ggcacggcga accggtagtt gtcccgggtg cgctccagcg gcttgtccgg gcgggtcagg
     9541 tcccagtctg acgcaatggg ctgctcgatc aggacggtgc ggtccttggc gccgcggttg
     9601 gcgatggagt agtcccgttc ccgctgcaac cggttggaga agtagaagac gcccttgccg
     9661 atccgcacgg agatgagggt cgtcggggaa gctccctgcg tggactggat ctcggtgtcc
     9721 agatcgagcg cgtacgtgat gagctgctcg gcgccggggg gcaggtccgt gatctgggcg
     9781 tccccggcgt agcttccccc gtcgaacacc gtgatggggc cctgcatgag atggagcgcg
     9841 ctcgcgttct tgagccgcac cgcgttgagc gggtgcgtcg gctccgttgc cgggttgtac
     9901 agcgagtagc gcgcgccgtc cacgagctgg ttcaggatgg ggagcatcgc ggactgctgc
     9961 cggggcagcg tcaccggctt gtcgatgacg tactggaaga gctctcccac cgcggagccg
    10021 gtggccgccg cgttcacgcc ctgggccggg ttgaaggact ccggagcggg agcggcgaac
    10081 cccagggcgg cgcggggccc ggcgggctgc gccatgctct tggccgcggg cgcggaggcc
    10141 ggggcaggcg cgcccgccgc ggtcgcctcc tccatcggcg cctcctggtc catcgccatc
    10201 tcgttggtct tggggaccag ggactgatag agctcgagct ggacctccgg ccgctggatg
    10261 tacagcggct gatagaggtc catggtgaac gtgatgggtc gcccggacac cagcttgagc
    10321 cccacctggc tccagtccgt gtccgtcgtg ttctcgacca gggcccaacc ctggaggagg
    10381 tgctgtccgg tttcggcgag gaccaaccga tagctggtct tccacacggg agactccagg
    10441 atgtagccga tccgaacccg ccgcttgccg gctcccgaga agtgcaggac cagcttcttc
    10501 ttctccacgc cgtgggaggc ggagagcacg aggagggcct gcgcaaggtc gtcctggatc
    10561 tcctttcgca ggaaccggat cccttctacg tcctggagcg cgatggaccg gacgccggcc
    10621 gcggtgctga tgttgacgaa gtccccggcc accacgccct tggggctggt ctgctcctgc
    10681 cgcgtctcca cgctgatgat ggtgccggtg accttgctgg cgttcgtgag ggtcacctcg
    10741 accgcttccc ctcgagtctg gacgagcagg ttggagaggg ttgggtccga ggtcaggtcc
    10801 acggcgaagg tcttcagggt ccgggtgatc gggtcccgtg aggagtaggt cacgctcgtg
    10861 atggagccgc cgtccagatc ccggaccacc aggctcttga ggagatcgtt gatctggctg
    10921 gcgctgaagg tcagctccat ctgggcgttg ccgtccacca cgccgtcgtg ctggaagtag
    10981 gtcacgccgc tcgtgaagag caccatgctg gagatgggga tggtgacggt cttttcggcc
    11041 gccgccgggg aggccgcctg cgcgcctgaa acgaacggtg ccaggaggcc ggccgccagc
    11101 aaagccgcgg cggcaagagt cacagtccgg cgcctcatgc tagtcctcct tctcccccgg
    11161 ggtggtggat ggcagagccc gagcggtgct ctctcgggcc gcaaagacga atccgaacag
    11221 gccgccaatg actcctccgg cgatgtgggc gagctgggag atggtgctgg gggtcagggc
    11281 gttcacgaac tcccgcacca ggtacagggc cacgatcagg atgaacgtaa tggggatgtc
    11341 cccctgacgg atgttggtga aggagctcag gaggatgagc atgaacacga ttccgctggc
    11401 tcccaggagg ccggtccgca ccaggaggat gttgaggatc ccggtggaga ccgcggtcac
    11461 cagcatcatc agcaacagcg gcacggagcc gtacttctcc tccaggatcg gcccgatcag
    11521 gaggatgaag gagaagttgc tgatgaggtg gttccagctc tggtgcccga tgacatagga
    11581 gaacagcgtg aaccacgaca ggagagaccg cgcgttgaag ccggggtaga tcgtgaagta
    11641 cgtgcggatc aggcttccac cggtgagctg gtcgatcagg agcaccaccg ccgcgatgat
    11701 ggtgaaggtc aggatcaccg gcgcgttgta gcggagtcga atgcccatgc ctatcctcct
    11761 cggtcccagt ataccgctag gacaaccggc gcgcgatctc ttcttccacc gcctgggcaa
    11821 acgcctccgc ggcgtccggc cgggtccccc tcccctggac ccgcgcgccc ttgcctccgc
    11881 ccttgcagcc gccgatggcg tacagcgggg gcaggagacc gcccaggtcc agacggtcgc
    11941 cgacggattg cgccaccagc cactggaatg cctccgccgt ccggtccgtg acgatgacca
    12001 cgcgcccggg tctggcgatc accccttcgg cgaagagctt gaggtagtcc gcgccggccc
    12061 cctgcagtcc gcggcggacg atggccgcgc caccgacggc ccgcgcggag gacgcggctt
    12121 gatcggcgtc cgcggccgcc agccgggtcg tgaggcggcg gatctcctgc gccatctcct
    12181 tgccgcgcgc ggagagctcc tccacgcgcg agagcatgtt ctcttcgccg caggtcagcg
    12241 ctcgggagag cgcctggacc agcgcggtct tgcgccgata gtccgccagc gctcgcgccc
    12301 cgatgaccgc gtgaatgcgc acccttcccc ggatccgctc ctgccctacc accttgatca
    12361 ggagcagctc tcccgcgttc cttacgtgca ctccgccgca ggccgcccag tcgaagccgt
    12421 ccacctccac gatccgaagc cgctgggcct tgggaggcgt tcgccgcaag gggtacttcg
    12481 agagctcgga ccggtcgatc tcgtgcaccc gcacgcccag gttccgccgg atggcgccgt
    12541 tggccagctc ttccgcggca tccagcacct ccgccctcac ctccggcacg gccagctcga
    12601 tcgtggtggt ctgctcgccg aagtgcacgg acacggtggg aaggtccccg gcgcggacca
    12661 gcgcctggga gaggatgtgc tgacccgtgt gctgggtcat gtagtccagg cggcgcgccg
    12721 catccaccac gcaccgcacc ggaccggcgg ggacggcgga ctccagcacg tggacgatct
    12781 catcgccttc atggcccgac tcatcgccct cct
//
LOCUS       DASRZS010000197         6050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_107544, whole genome shotgun sequence.
ACCESSION   DASRZS010000197 DASRZS010000000
VERSION     DASRZS010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107544"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFH83_04895"
     CDS             complement(<1..321)
                     /locus_tag="VFH83_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6485732.1"
                     /translation="MHRDILAQLLSDIGRVRMAVIGDFCLDAYWLLDASLAEMSIETG
                     LETRAVRSQRYSLGGAGNVVSNLVALGVKAVSVFGVVGDDPFGREMLRILSAASVDTR
                     GMRFQ"
     gene            416..2569
                     /locus_tag="VFH83_04900"
     CDS             416..2569
                     /locus_tag="VFH83_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HET6485733.1"
                     /translation="MTTVHMIGNAHIDPAWLWRLTAGVGEVLSTCRTVCDLLDREPGF
                     IFTRSDVWVYERVESLDPELFQRILRHIAEGRWAVVGGWYIQPDCNLPRGDSFQKHIE
                     VGRRYSKDRLGVTATVGYNVDSFGHAASLPRILSDNGYDSYVMMRPGAHEMVLPSALF
                     RWQSPPEAGGGGSVVVWRIPSAYGTGAADLSDQVRAALAAAVPGIDHVMCFYGVGDHG
                     GGPTLEQIRWIREHERSFDGARLLFSHPRAFFDAVRGSAGSLPLVVGELQMHAVGCYT
                     VGRRIKEEMRRAEHGIHMAETAVRAFPDSAPVEAAAELGRAWSHALFNQFHDIYGGTC
                     IPPACDDAVAQLASARETARSVAYNTLFRHAVSLPPARRQRVVAFNGSQRPFKGFIRW
                     EPWMGWGKPFDGWISDENDSPVLSQVVSSSSVSGLWGALLWPAQMGPGSLRQFRLQSK
                     PGPRLPASDVRAGESIIESGSWTAGPGSGGSLLAIQNRVGGPLIAAPGIRLVLMEDLS
                     DTWSHGVRGYEGKTVGEFSVQAVTLEESGPVRATLRVDAKAENSSLTLFARVYSGDPR
                     LELELLIDWRERLRLAKLVFTLAGPVASRLDGIPGGALNRAQDGRELPLLDWSLAGLP
                     GGRSLGFAAPDCSALDGRGDALRFTLLRSPVYAWHIPTVLPPGRAYPFMDQGEHRFRF
                     LLVPDASPSVVEDLALSEHRAPLCIDWTKGMGDGA"
     gene            2566..3978
                     /locus_tag="VFH83_04905"
     CDS             2566..3978
                     /locus_tag="VFH83_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6485734.1"
                     /translation="MTYAEIMARTRSLAARPALPPDPARFQRVLAEFMARTGGSARRF
                     QQAGQVLPRGNEHAWAPSVPYPLFMERGQGSRVRDVDGNEYVDYILAGGPLILGHNPE
                     GLRRSMQELIGKRTWFHGYHDEMEILAGQKILQHFPGIQRVRFTTSGTEANAAAARIA
                     RSFTGKKKIAKYLAGYHGWSDAFVTDLEIPGSGRILSQGIPAEVLDLTVLVPPNDLEA
                     LDRALAAHAADGGIAAVFCEPIGAESGLVPFREGFHRDAMEIAHRHGALYVFDEVVTG
                     LRMGLGGAQAALGVKPDLTTLGKALMGGWPGCGAVGGRADVMETASAGVMPDGKPFAY
                     LAGTFTGNTLSAAAAYYTFLELEKPGVLDGMFAAATDYVEKLNRLFAARGCGFFAYAF
                     GGILRIEMAAAHGVPLDGPGAFQEIVQRRTLIEQYSVVLHARGVLSRNGRDMVSCSHT
                     TQDNDLGVAAFASLIDILGA"
     gene            3986..4795
                     /locus_tag="VFH83_04910"
     CDS             3986..4795
                     /locus_tag="VFH83_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6485735.1"
                     /translation="MMVQSKGRVLVTGISRGIGKAIAQALSQDGWHVIGTSRDPTAVP
                     DAQRLPGVEYLPLDLCQERSITDLANQVGAVDVLVSNGGASMISPAEETPLNKVRDLF
                     EADFFGPVLLTQALLPDMRRREGGRLIYIGSMRGEAPSPFSAFYSAAKAALRSFAQCL
                     RMEVRRYGLRVSVIAPWHIRTALALEKLHADTSAYAEAVDRVRKKRDEGIAEASDPVV
                     VARLVQRILASRRPRAFYSVGRMGRSQAFLVKHLPRSLVEAMSRRRFEPKG"
     gene            complement(4810..5904)
                     /locus_tag="VFH83_04915"
     CDS             complement(4810..5904)
                     /locus_tag="VFH83_04915"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HET6485736.1"
                     /translation="MPTELLFARYQAATMEPKDTIGAKWSRLLDQLDLGSAVRGRRTA
                     IKTHLGGGVGFTTIHPFLLRTLVTKVKEAGARDVFVTDTHYGVRSAVERGYTEEVLGC
                     KLLPIAGPEDKEIVTKAVEPAFKALSSLNLGAEVMRAEALVDLSHVKGHGACGFGGAS
                     KNLSMGCVDQKTRGALHGLEGGLEWDPQECTLCKTCAENCPNAAISFPKDQFEVFYHN
                     CKLCQHCVLICPQKAITVKGGEFADFQKGLALATTAVLRGFPPGAVVFINMVMNVTVF
                     CDCWGITTPSLVPDVGILAGRDIVAIEQASLDLVRVENLIPGSLPPTWKLQEGNHLFQ
                     RIHGKDPFIVVQQLQEMGWGSRDYALREVC"
BASE COUNT          970 a         1982 c         2060 g         1038 t
ORIGIN      
        1 ctggaaccgc atgccccggg tatcgacact ggcggcggag aggatccgca gcatctcccg
       61 gccgaacgga tcgtctccca ccacgccgaa gacgctcacg gccttcacac ccagggccac
      121 caggttgctc accacgttgc cggcgccgcc cagggagtag cgctgacttc ggaccgcccg
      181 ggtttccaga cccgtctcta tcgacatctc ggcgagggaa gcgtccagga gccagtaggc
      241 gtccaggcag aagtcgccga tgacggccat gcggacacgg ccgatgtccg agagcagctg
      301 cgcgaggata tcccggtgca tggtgagtcg gaagtgtagc ggcgacggac gggcggatca
      361 aggctggccg cccggaccgg ttgccccgcg gagagggttt ctctatagtc ctgccgtgac
      421 aacggtacac atgatcggga acgcccatat cgatcccgcg tggctctggc ggttgacggc
      481 cggcgtgggc gaggtcctgt cgacctgccg gaccgtgtgt gaccttctgg atcgggagcc
      541 tggcttcatc ttcacccggt cggacgtctg ggtctacgaa cgcgtggaga gcttggaccc
      601 cgagctgttc cagcggatcc tccgtcacat cgccgagggc cgatgggcgg tggtcggcgg
      661 atggtatatc cagccggact gcaaccttcc ccggggggat tccttccaga aacacatcga
      721 ggtgggccgg cggtactcga aggaccgctt gggcgtcact gccacggtag gctacaatgt
      781 ggacagcttt ggccacgccg catcgcttcc ccggatcctg agcgacaacg gatacgacag
      841 ctacgtcatg atgcgtccgg gcgcccacga gatggtcctc ccaagcgcgc tcttccgctg
      901 gcagtcgccg ccggaagccg gaggcggggg ctcggtggtc gtctggcgaa tccccagcgc
      961 ctacggcacc ggagctgccg atctttccga ccaggttcgc gcggcgctgg cggccgcggt
     1021 gcccgggatc gaccacgtca tgtgcttcta cggcgtgggg gaccacggcg gcggcccgac
     1081 cctggagcag atccgctgga tccgagagca cgagcgttcc ttcgacgggg cgcggctctt
     1141 gttctcccac cctcgggcgt tcttcgacgc cgtgcggggc agcgccggct ccctgccgct
     1201 ggtagtggga gagctccaga tgcacgccgt gggctgctac accgtagggc ggcggatcaa
     1261 ggaagagatg cgccgcgccg aacatgggat tcacatggcg gagaccgcgg tgcgcgcgtt
     1321 cccggactcc gctccggtcg aagcggcggc ggaactgggc cgggcgtgga gccacgcgct
     1381 cttcaaccag ttccacgaca tctacggcgg gacctgcatc cctccggcgt gcgacgacgc
     1441 cgtggcccag cttgcgagcg ccagggagac cgctcgttcc gttgcgtaca acacgctatt
     1501 ccgccacgcg gtcagcctgc cgcccgcccg ccgacagcgg gtcgtggcct tcaacggatc
     1561 ccagcggccc ttcaaaggat tcatccggtg ggagccctgg atgggctggg gcaagccctt
     1621 tgacggctgg atctcggacg agaacgattc gcccgtcctt tcccaggtgg tttcttcctc
     1681 ttccgtgtcc ggcctctggg gcgcgcttct gtggcccgcc cagatgggac ccggatccct
     1741 tcggcagttt cggcttcaaa gcaagcccgg gccgcggctt cccgcttcgg atgtgcgcgc
     1801 gggggagtcg atcattgaga gcggttcgtg gacggcgggt ccaggctcgg gcggctcgct
     1861 gctggccatt cagaacaggg tcggcgggcc gctcatcgcc gccccgggga tccgcctcgt
     1921 cttgatggag gatctatccg atacctggtc ccatggggtt cggggatacg agggaaagac
     1981 ggttggagag ttctcggtcc aggcggtcac gctggaggaa tcagggcccg ttcgcgccac
     2041 gttgcgggtc gatgccaaag ccgagaacag ctcgctcacc ctgtttgccc gggtatactc
     2101 gggagatcct cggctggagc tcgagcttct catcgactgg cgcgaacggc ttcgcctcgc
     2161 gaagctcgtc ttcacccttg ccggtccggt tgcctctcgg ctcgacggga ttcccggcgg
     2221 cgctctgaac cgcgctcagg atggaagaga gcttcccctc ctcgactgga gcctggccgg
     2281 gctccccggc ggacggtccc tcggcttcgc agcgccggat tgctccgcgc tggacggtcg
     2341 gggggacgcg ctccgcttca cgctcctgcg ctccccggtc tacgcctggc acatcccgac
     2401 ggtgctgcct ccgggacgcg cctacccgtt catggaccag ggggagcatc ggttccggtt
     2461 cctcctggtc cctgacgcct ccccctccgt cgtggaggac ctggcgttgt cggagcatcg
     2521 ggcgccccta tgcatcgact ggaccaaggg catgggagac ggagcatgac gtacgccgag
     2581 atcatggccc ggactcgctc gctcgcggct cgccccgcgc tgccgccgga tcccgcgcgg
     2641 ttccagaggg tgctcgcgga gttcatggcg cggaccggcg gctccgcgcg acgcttccag
     2701 caggcggggc aggtcctccc ccgcggcaac gagcacgcct gggcccccag cgtgccctac
     2761 ccgctcttca tggaaagagg gcaaggcagc cgggtgcggg acgtggacgg caacgagtac
     2821 gtcgactaca tcctggccgg cggaccgctg atcctgggac acaaccccga ggggctgcgg
     2881 cggagcatgc aggagctcat cggcaaacgc acgtggttcc acggctacca cgacgagatg
     2941 gagatcctgg cgggccagaa gatccttcag catttccccg gcatccagcg agtccgcttc
     3001 acgacttccg gcaccgaggc caacgcggcg gcggcccgca tcgcgcgctc gttcaccggg
     3061 aagaagaaga tcgccaagta cctggccggc tatcacggct ggagcgacgc gttcgtgacg
     3121 gacctggaga tccccggcag cgggcgcatc ctctcccagg gcatcccggc ggaagtgctg
     3181 gacctgaccg tgctggttcc gccgaatgac cttgaggccc tggaccgcgc gctggcggct
     3241 cacgcggcgg atggcgggat agcggcggtc ttctgcgagc ccatcggcgc cgagtccggc
     3301 ctggttccgt ttcgggaagg gtttcaccgg gacgccatgg agatcgccca ccggcacggc
     3361 gcgctgtacg tgttcgacga ggtggtgacc ggactccgca tgggtctggg aggagcccag
     3421 gccgctctgg gcgtcaagcc cgatctcacc acgctgggca aggcgctcat gggcggatgg
     3481 ccgggttgtg gagcggtggg ggggagggcg gacgtcatgg agaccgccag cgcgggcgtg
     3541 atgccggacg gcaagccctt cgcctatctg gcggggacct tcaccggcaa caccctttcc
     3601 gcggcagccg cttactacac gttcctggag cttgagaagc cgggcgtcct ggatgggatg
     3661 ttcgcggcag cgaccgacta cgtggagaag ctgaaccggc ttttcgccgc tcggggctgc
     3721 gggttcttcg cgtacgcctt cggcgggatc cttcggatcg agatggccgc cgcgcacggg
     3781 gtcccgctgg atggcccggg ggcattccag gagatcgtcc aacgcaggac cctgatcgag
     3841 cagtactccg tggtgctgca cgcgcgggga gtcctgagcc ggaatggccg ggacatggtc
     3901 tcctgcagcc acacgacgca ggacaacgat ctgggggtag ccgcctttgc atccctcatc
     3961 gacatcttgg gcgcgtagga gcggcatgat ggtccagagc aagggcaggg tcctggtcac
     4021 ggggatctcg cggggcatcg gaaaggcgat tgcccaggca ctgagccagg acggctggca
     4081 cgtcatcggg acctcccgag atccgaccgc cgttccagat gctcaacgct tgcccggcgt
     4141 cgagtacctg ccgctggatc tgtgccagga gcgatccatc accgacctgg cgaaccaggt
     4201 gggcgcggtg gacgttctgg tgagcaacgg cggcgccagc atgatcagtc cggccgagga
     4261 aacgccgctg aacaaggtga gggacctgtt tgaagcggac ttcttcggcc ccgtcctcct
     4321 cacccaggcg ttgctgccgg acatgagacg ccgtgagggc ggcaggctga tctacattgg
     4381 gtccatgcga ggcgaggctc cctcgccgtt ctccgctttc tactcagcgg ccaaggcagc
     4441 gctgcgctcc ttcgcccaat gcctccgcat ggaggtgcgg cgctacggtc tgcgggtctc
     4501 ggtgattgcg ccgtggcaca ttcggaccgc cctggcgctg gagaagctcc acgccgacac
     4561 ctcggcatac gctgaagcgg tggatcgggt gcgaaagaag cgggatgagg gaatcgcgga
     4621 ggccagcgac ccggtggtgg ttgcgcggct ggtgcagcgc atcctcgcct cccggcgccc
     4681 cagggcgttc tattccgtgg ggcgcatggg tcggtcccag gcgttcctgg tcaagcacct
     4741 cccccggtct ttggttgagg cgatgtctcg tcgaaggttc gaaccgaagg gataacgccg
     4801 aaccgcgcct cagcagacct cgcgaagcgc atagtcgcgg ctgccccacc ccatctcctg
     4861 gagctgctga accacgatga aggggtcctt gccgtggatg cgctggaaga gatggtttcc
     4921 ttcctgcagc ttccaggtcg ggggaagcga tccggggatc aggttctcca cccgcaccag
     4981 gtccagagac gcctgctcga tggccacgat gtcccggccc gccaggatac ccacgtccgg
     5041 cacgagggac ggtgtggtga tgccccagca gtcacagaac accgtcacgt tcatcaccat
     5101 gttgatgaag acgaccgcgc ccggcgggaa tccgcggagc accgcggtgg tggcgagcgc
     5161 aaggcccttc tggaagtcgg cgaactcccc gcctttcacc gtaatggcct tctgggggca
     5221 gatcaggacg cagtgctgac acagcttgca gttgtggtag aagacctcga actggtcctt
     5281 gggaaaggag atggccgcgt tcgggcaatt ctcggcgcag gtcttgcaga gggtgcactc
     5341 ctgggggtcc cactccagcc caccctccag gccgtgaagg gctccgcgcg tcttctgatc
     5401 cacgcagccc atggacagat tcttcgaggc gccgccgaac ccgcaggcgc catggccctt
     5461 gacgtgggac aggtctacca acgcttcggc ccgcatcacc tctgccccca ggttcaggga
     5521 ggaaagagcc ttgaatgccg gctcaacggc cttggttacg atctccttgt cctcgggccc
     5581 ggcgatcggc agaagcttgc agccaaggac ctcctcggta tacccgcgct ccaccgctga
     5641 gcgcactccg tagtgcgtat cggtgacgaa gacgtcccga gcccccgcct ccttcacctt
     5701 ggtgacgagg gtgcgcagga ggaacggatg gatcgtggtg aaaccgacgc caccccccag
     5761 atgggtcttg atggcggtgc ggcggccgcg gaccgcggag ccaaggtcga gctggtccaa
     5821 gagtcgcgac cacttggcgc caatcgtgtc ctttggctcc atggtggcag cctggtaccg
     5881 agcaaagagt agttctgtgg gcatatgaaa acctcatacc agagttgtca gggttgtgca
     5941 agcaccatta tctccattct tgcacgggtt ttcgcgcgac acaccgtggg tgaacgcgcg
     6001 caaaggtacg cacctacgga ggctgttgcg cgccgaatcc cagtgtcgtt
//
LOCUS       DASRZS010000198         2971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5496918, whole genome shotgun sequence.
ACCESSION   DASRZS010000198 DASRZS010000000
VERSION     DASRZS010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5496918"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFH83_04920"
     CDS             complement(<1..365)
                     /locus_tag="VFH83_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HET6485737.1"
                     /translation="MEGTRVKLAIAPIAWTNDDLPELGGGNTFEQCVSEMALAGFQGS
                     EVGNKYPRDPRVLNHHLGLRGLTICNAWFSAYLTTKPYQEVESEFLAQCDFLYAVGAR
                     IIGAAEQGRSIQGIQTQPV"
     gene            complement(375..1382)
                     /gene="iolG"
                     /locus_tag="VFH83_04925"
     CDS             complement(375..1382)
                     /gene="iolG"
                     /locus_tag="VFH83_04925"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007132241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HET6485738.1"
                     /translation="MNVGLIGAGRIGKIHAENIAHHLGRARLTRIADIAADPLREWAR
                     GLGVEKVSTDYREVLEDPRIDAVIVTSSTDTHADLVVAAAERGKHVFCEKPIALTVPN
                     VRRALAAVSRAGVKLQVGFNRRFDHNFSRVQKLVREGSIGQPQIVRITSRDPAPPPPQ
                     YVAVSGGIFLDMMIHDFDMARFLSGSEVTEIFARGAVLIDPAIGAAGDVDTAVVSLRF
                     ENGALGTIDNSRKCDYGYDQRVEVFGSKGSLACSNDTPSSVVLSTTGGALAEKPLYFF
                     LERYRQAFVDEMKSFVDAVLDGTPTPVNGNDGLASLLLGLAAKKSLAEGRSVTVSEVA
                     G"
     gene            1464..>2971
                     /locus_tag="VFH83_04930"
     CDS             1464..>2971
                     /locus_tag="VFH83_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HET6485739.1"
                     /translation="MATSTQKQVPARNQGLRAPKKDTQGLSVSLVTGADEPALRSLIG
                     KSVAVSSGKGGVGKTITACNLAIYYARRGLRVGLVDVDPLSDVATLLDLQESEQALEG
                     GAARDDGLEGNLLPVFKGLEVVFPAQKLERTEISGLMGRIYRRHLKEIDKRYDLVIFD
                     MPAGMDHEENLAYLPFMKRLILVTTPEPTSHASAGAYVREVQRLYPGTPIKVWHNRYS
                     SRMKDGFHPSDVAGNYNRFVDPEERLSAEQCAALEDFAFVPEDPAMDLLKGEPDPTIH
                     VLACMKDSLDYAHGRLLSQAARRLGFSKGVHEIIATYVQHHPRLDNPEKYLSGLSEHL
                     NAIIQAVADPHALNVTNQPARAKPFNEQERQTILTFLQRVQQSALRKQILDLEEVLQE
                     RVRAMESSRGPFAGGVPVGQDPGLDRELARFLMALGKSARGNTLVRNQGVLLLFYFCL
                     HKLFQSKTLLKLLKGLIPRRANRRGRMVRDRLQQIRNLVEREPQYRQRYLKT"
BASE COUNT          522 a         1007 c          949 g          493 t
ORIGIN      
        1 aaaaccggct gggtctggat gccctggatg gatctgccct gctccgcggc gccgatgatg
       61 cgcgcgccga ccgcgtagag gaaatcgcac tgagccagaa actcgctctc tacctcctgg
      121 tatggcttcg tggtgaggta ggcgctgaac caggcgttgc agatggtgag cccgcgaagt
      181 cccaggtggt ggttcaggac cctcgggtcc cgcggatact tgttccccac ctcgctgccc
      241 tggaaaccgg ccagggccat ctccgacacg cactgctcga acgtgttccc cccgcccaac
      301 tcgggcaggt cgtcgttggt ccaagcgatc ggcgcgattg ccagctttac ccgagtgccc
      361 tccatggcga gctcctatcc cgcgacctcg gagaccgtta cggaccggcc ctcggcaaga
      421 gacttcttcg ccgcaagccc caggagcagg ctggccaggc cgtcgttccc attcacgggg
      481 gtaggcgtgc cgtccaggac ggcgtcgacg aaggacttca tctcgtccac aaacgcctgt
      541 cgatagcgtt ccaggaaaaa gtacagcggc ttctccgcga gcgctccgcc cgtggtggag
      601 agcaccacgg aggacggcgt gtcgttcgag caggccaggc tgcccttgga tccgaagacc
      661 tccacgcgct ggtcgtagcc gtagtcacac tttcgggagt tgtcgatggt gccaagggcg
      721 ccgttctcga agcgaaggga gaccacggcg gtgtccacgt ctcccgcggc gccgatcgcc
      781 gggtcgatca ggaccgcgcc ccgagcaaag atctcggtca cctcggagcc ggacagaaag
      841 cgcgccatgt cgaaatcgtg gatcatcatg tccaggaaga ttccgccgga caccgccacg
      901 tattggggtg ggggaggcgc cggatcccga gaggtgatgc gaacgatctg cggctgccct
      961 atcgatcctt cgcggaccag cttctggacc cgactgaagt tgtggtcgaa tctgcggttg
     1021 aagccgacct gcagcttcac gccagcccgg gagacggcgg ccagggcccg gcgcacgttg
     1081 ggaacggtga gcgcgatggg cttctcgcag aagacgtgct tgccacgctc cgccgcggcc
     1141 accaccaggt ccgcgtgggt gtccgtggag ctggtgacga tcaccgcgtc gatccgcgga
     1201 tcctccagca cctcccgata gtccgtgctg accttctcca cgcccaatcc gcgcgcccac
     1261 tcccgcaggg gatccgccgc gatatcggcg atcctcgtga gcctggccct gccaagatga
     1321 tgtgcgatgt tctccgcatg gatctttccg atcctgcccg cgccgatcag cccgacgttc
     1381 atcccggccc cctctggctc tgtgatagca ggttgacgcc cacccggcaa ggcaggctat
     1441 gatgggcggt cgtcgagcgg cggatggcta cgagcacgca gaaacaggtt cccgcacgga
     1501 accagggcct acgcgccccg aagaaggaca cccaagggct ttccgtcagt ctggtgacgg
     1561 gcgcggacga gcctgctctc cgctccctca tcggcaagtc ggtggccgtg agctcgggca
     1621 agggaggcgt cggcaagacc atcacggcct gcaacctcgc catctattac gcccgtcgcg
     1681 ggcttcgggt gggcctcgtc gacgtggacc ccctctcgga cgtggccacc ctcctggacc
     1741 tgcaggaatc cgagcaggcc ctggaaggcg gggcggccag ggacgacggc ctggagggca
     1801 atctgcttcc ggtcttcaaa ggcctggagg tcgtcttccc ggcccagaag ctggaacgga
     1861 cggagatcag cggtctcatg gggcggatct accgccggca tctcaaggag atcgacaagc
     1921 gctacgacct ggtgatcttc gacatgcccg cgggcatgga ccatgaggag aacctggcgt
     1981 atctcccgtt catgaagcgg cttatcctgg tgaccactcc ggagcccacc tcccacgcgt
     2041 cggccggcgc ctacgtgcgc gaggtccagc gcctctatcc cgggacgccc atcaaggtgt
     2101 ggcacaaccg gtactccagc cgcatgaagg acgggttcca cccctccgac gtggcgggga
     2161 actacaaccg gttcgtggac ccggaggaga ggctctccgc ggagcagtgc gctgcgctgg
     2221 aggacttcgc gttcgttccg gaagaccctg ccatggacct tttgaagggc gagccggatc
     2281 ccaccatcca cgtgctcgcc tgcatgaagg acagcctgga ctacgcgcac ggccgtctcc
     2341 tctcccaggc ggcgcgcagg ctgggcttct ccaaaggcgt gcacgagatc atcgccacgt
     2401 atgtccagca tcatccccgg ctggacaacc ccgagaagta cctgagcgga ctctcggagc
     2461 acctcaacgc catcattcag gcggtggcgg acccgcacgc gctgaacgtc accaatcagc
     2521 cggcccgcgc gaagccgttc aacgagcagg agcgccagac catcctcacg ttcctgcagc
     2581 gcgttcaaca gagcgcgctg cggaaacaga tcctggacct ggaggaggtc ctccaggagc
     2641 gcgtccgggc aatggagtcc tcccgcggtc cgtttgccgg gggtgtcccc gtggggcagg
     2701 atccgggatt ggaccgcgag ctggcgcggt tcctgatggc cctcgggaag tcggcgcggg
     2761 ggaacacgct ggtccgaaac cagggagtgc tcctgctctt ctacttctgc ctccacaagc
     2821 tcttccagtc caagacgctc ctgaagctcc tgaaggggct gatcccccgg cgggccaatc
     2881 gccgcggccg catggtccgc gaccggctcc agcagatccg aaacctcgtg gagcgggagc
     2941 cccagtatcg ccagcgctac ctcaagacca t
//
LOCUS       DASRZS010000199         7152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5497035, whole genome shotgun sequence.
ACCESSION   DASRZS010000199 DASRZS010000000
VERSION     DASRZS010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5497035"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VFH83_04935"
     CDS             complement(<1..870)
                     /locus_tag="VFH83_04935"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HET6485740.1"
                     /translation="MASPDPGLSLYLHVPFCTDKCLYCDFFSVPRGSVPPSVITRVVE
                     QTVELAQTLWDASGRAPLRTLFIGGGTPSCLPPSLLRRLLGPFVGQGVVEWTVESNPE
                     TLSQAFLDECEAAGVTRLSLGIQSLSSELLQRVRRRATREQSLSAIDLLRRKWRGQVN
                     LDFITGIPGQTQDDVGECLSVIQPQWPDHVSLYQLTVEPGTPLEAGVADRSIRLNEPE
                     KDEDLWLAGREGLLSRGFRQYEVSNFARPGRECRHNVRYWMIQPYAGAGPGAVSTIPS
                     AWASRLAGLMLPTA"
     gene            complement(857..1609)
                     /gene="lepB"
                     /locus_tag="VFH83_04940"
     CDS             complement(857..1609)
                     /gene="lepB"
                     /locus_tag="VFH83_04940"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET6485741.1"
                     /translation="MIGSVERYSPAAGKRRAGPLGGRLLRVVLIGLLLYLVVSRFIAG
                     SYRIESVSMEPSLLPAQRVIVSLIAYGPRVPYSLARLPGMGLPQRGDLVVVQPPFVSD
                     ASLVLKIAEPVASFFSLQKLTLFRDPFGSPVTSYMVKRVVGLPGDTIRISHFSVSVRP
                     RGGTDFVPEQQMVPVTYTVQGSMPARGWSEQLPLSGTRNGDLLLDDQQYFVLGDNRPS
                     SSDSRSWGPVRLERIVGKVVYRYWPPAAIGIP"
     gene            complement(1616..2674)
                     /gene="lepB"
                     /locus_tag="VFH83_04945"
     CDS             complement(1616..2674)
                     /gene="lepB"
                     /locus_tag="VFH83_04945"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET6485742.1"
                     /translation="MAEPAPGGSVSWESWELWLVRVTERYLSWRRKRRLERKARQKKR
                     NQVVDWLDAILSAVVIVLVINQYLLQAYQIPSPSMEPSLLVTDRIFVNKIVYGPELIP
                     GMLKIPGPWKPQRGDVIIFESPEYISSGPVVDILQRIIYMVTLSLVDIDKDENGQPKH
                     HFLIKRAIGMPGDRIRMNEGNVEILPPGASAWIAEPDMKKAMGFDYQNVRIPSVSGDY
                     PVFKEAGIAAGLVGEKLRATTEEEAAVKKYYRTTSDSSGTVTGVGPMMQLADDIYVQM
                     WTDATRWAIEPDNLAAGAEWRMLAEGYYVPEGRLFPMGDNRDNSHDARYFGPVRLEKV
                     LGKALFRYWPLYRIGGVR"
     gene            2750..4381
                     /locus_tag="VFH83_04950"
     CDS             2750..4381
                     /locus_tag="VFH83_04950"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET6485743.1"
                     /translation="MSKKDRNEPPSTAADAGVRLKPVLGVRPGQYLTVIYAVVLVLAV
                     YLLLFVPGLRHRGAYLSLQSFPEHAAVTVDGRYAGSTPCTVFLPHGSRTVMVSRPFYT
                     AVTLTRKVAGRVFATLLVPDRSRASVSLDVADVNGLAAGALADFQKNPQIPQIVSQAA
                     WAAAANSRDPLYDLVARSLLVTTDESQLREILVAAGRVSARTGFLTAGSLLDMVQRAA
                     AFAESADNAPAWLLLTLSRANQTHIAASPWVQQYLSGYRDVLSRYYQPGAPVSGPAGG
                     GPVVAGIAFRGIPGGDLVMGRDDELDTLGKTIDRLLAHPVRVAPFFLASSEVTNAEFQ
                     AFVAENPDWAPSNGAALIQKGVVTDTYLAQWKSGAPAAGTANTPVTSVSWYAAEAYCQ
                     WLTRRVQASLPGYQARLPSESEWEWAARGGLRGMPYPLGGKPGGAVFFSPGITGPSAS
                     GTSEPNGYGLRDMLGNVWEWCSDPFSLNASVLSSRDPKSSAQLEASLPDAPDRAVRGG
                     SWADQPGADKVYTRGSQPAQWCTPYLGFRVALGRR"
     gene            4425..4886
                     /gene="smpB"
                     /locus_tag="VFH83_04955"
     CDS             4425..4886
                     /gene="smpB"
                     /locus_tag="VFH83_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET6485744.1"
                     /translation="MAKSEGIKILAVNKKARFNYSIDESFECGVVLVGTEVKSMKEGR
                     FSFADAYASVENDELWLRDLHVTPYAFGNRINHEPLRKRKLLVHRKEIKRLKRITDEK
                     GLTLVPLKFYLSRGYVKCELGIARGKKLFDKRDDIRKRDQKRELEREFRVK"
     gene            4990..5339
                     /gene="ssrA"
                     /locus_tag="VFH83_04960"
     tmRNA           4990..5339
                     /gene="ssrA"
                     /locus_tag="VFH83_04960"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5318..5500)
                     /locus_tag="VFH83_04965"
     CDS             complement(5318..5500)
                     /locus_tag="VFH83_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485745.1"
                     /translation="MVAHHGILEDGIDFIGKPYSPAEQTAWVRQVLDRKLGARDGIGA
                     NGARARRAPRDGGGGS"
     gene            complement(5567..5805)
                     /locus_tag="VFH83_04970"
                     /pseudo
     CDS             complement(5567..5805)
                     /locus_tag="VFH83_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003547236.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(5914..6084)
                     /locus_tag="VFH83_04975"
     CDS             complement(5914..6084)
                     /locus_tag="VFH83_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485746.1"
                     /translation="MKDTESRFPIANEANAALLCLSSDHAAVGRKDSAFYPAEGAARF
                     RADEVRVIETAA"
     gene            6389..>7152
                     /locus_tag="VFH83_04980"
     CDS             6389..>7152
                     /locus_tag="VFH83_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HET6485747.1"
                     /translation="MLKNIKIGTKLVTVGALLIAIPLAVVAFLSVQRASLAVMSIESE
                     QLARAARLIATSINNVFLEEQKVALNNATDNSIIETAVAVHGSAANAPKGKSAPSAAD
                     LIAVSTARIKATTGLGKDYESIMVVGTDGVAFASTDPAYVGGNFSDRAYIHEALAGKA
                     TAGTAVISKVTNKPVVPVAAPIKDGRNVVGAYVLVLDAQFLNDLVTGEKVGKTGYAYV
                     VDNTGLVIAHPVAANLFKTNLAEMDGTKAFTKRMIAG"
BASE COUNT         1267 a         2348 c         2294 g         1243 t
ORIGIN      
        1 ggccgtcggg agcatgaggc ctgcaaggcg ggacgcccag gctgacggga tcgtggagac
       61 ggcgcccggt ccggcccccg cgtagggctg gatcatccaa taccggacgt tgtggcggca
      121 ctcccttccc ggccgggcaa agttggagac ctcgtactgg cggaagcccc gggacaggag
      181 cccctcgcgc ccggcgagcc acaggtcctc gtccttctcc ggttcgttca gacgaatgga
      241 acggtcggca acgccggctt ccagcggcgt tcccggttcc accgtgagct ggtacagcga
      301 gacatgatcg ggccattgcg gctgaatcac ggaaaggcat tcccccacgt cgtcctgtgt
      361 ctgtcccggg atgccggtga tgaaatcgag gttgacctgc ccgcgccatt tcctgcgcag
      421 cagatcgatc gccgagagag actgctcgcg ggtcgcgcgc cgccggacgc gctgaaggag
      481 ctcggagctg agggattgga tccccaggct gagccgggtc acgcccgccg cctcgcactc
      541 gtcgagaaac gcctggctca gtgtctcggg attggattcc acggtccatt cgacaacgcc
      601 ctggcccacg aacgggccga ggagccgccg caggagagaa ggcggcagac agctcggggt
      661 gccgcccccg atgaacagcg tgcgcagggg tgcacgcccg gaagcgtccc agagggtctg
      721 tgccagctcc acggtctgct ccacgacacg ggtgatcacg gacgggggga cagagcctcg
      781 cggcaccgag aagaagtcgc agtacagaca cttgtcggtg cagaagggga cgtggagata
      841 gagggagaga ccggggtcag gggatgccaa tggcagccgg cggccagtag cggtagacca
      901 ccttccccac gatccgctcc aggcgcaccg gtccccagga cctgctgtcg ctggagctcg
      961 gtcggttgtc cccgagcacg aagtactgct ggtcgtccag gaggaggtcc ccgtttcttg
     1021 ttccggaaag aggaagctgc tccgaccagc cccgggccgg catcgagccc tggaccgtgt
     1081 acgtgaccgg caccatctgc tgctccggga cgaagtcggt gccgccccgg ggccgtacgg
     1141 acaccgagaa atgggagatg cggatcgtgt ctcccggcag ccctacaacg cgcttcacca
     1201 tgtagctcgt gaccggcgaa ccaaaggggt cacggaaaag cgtgagcttc tgcagggaga
     1261 agaagcttgc caccggctcg gcgatcttca ggaccaggga tgcatcggag acaaagggcg
     1321 gttggaccac caccaggtcc ccgcgctgcg gcagtcccat tcccggcagc cgtgccaagc
     1381 tgtagggaac ccgcggtccg tacgctatga ggctgacgat cactcgctgc gcggggagca
     1441 gagatggctc catggacact gactcgattc gatagcttcc ggcgatgaac cgtgaaacga
     1501 ccaggtacag gagcagcccg atgagcacga cgcgcagcag tcgcccgccc aggggccccg
     1561 cccgtctctt tcccgcggcc ggcgagtatc gctccaccga gccgatcatc cagtcctacc
     1621 tgacgccccc gatgcggtac agaggccagt agcggaacag cgccttgccc agcacctttt
     1681 ccagtcgtac cggtccgaag taccgggcat cgtgcgagtt gtcccggttg tcacccatgg
     1741 gaaacaggcg gccctctgga acgtagtagc cttccgcgag catccgccat tccgctccgg
     1801 ctgccaggtt gtccggctcg atggcccatc gcgtggcatc ggtccacatc tgcacgtaga
     1861 tgtcgtccgc gagctgcatc atcggtccca cgccggtcac cgtgccgctg gaatccgacg
     1921 tggtgcggta gtacttcttg accgcggctt cctcttcggt ggtggcccgt agcttctccc
     1981 cgacaagccc ggccgcgatc cccgcctcct tgaacacggg ataatcgccg gagacggaag
     2041 ggatccgaac gttctggtag tcgaacccca tcgccttctt catgtcgggt tctgcaatcc
     2101 aggcggaggc cccgggcgga aggatctcca cgtttccttc attcatacgg atgcggtccc
     2161 ccggcatccc gatggcgcgc ttgatcagga agtggtgctt gggctgcccg ttctcatcct
     2221 tgtcgatgtc cacaagggag agcgtcacca tgtagatgat gcgctggaga atgtcgacaa
     2281 ccggaccgct ggagatgtat tccgggctct cgaagatgat cacgtctccg cgctgcggct
     2341 tccagggccc ggggatcttc agcatgccgg ggatcagctc cgggccgtag acgatcttgt
     2401 tcacgaagat gcggtccgtg accaggagac tcggttccat ggagggggac gggatctggt
     2461 acgcctgcag gaggtactgg ttgatgacga gaacgatcac gaccgcggag agaatggcat
     2521 ccagccagtc caccacctgg ttgcgcttct tctgccgggc tttgcgctcc agccgacgct
     2581 tgcgccgcca gctcaggtac ctttcggtga cccgtacgag ccacagctcc cacgattccc
     2641 atgaaacaga gccaccgggt gccggttccg ccatcacgtt ctccggcgga acagtagcac
     2701 gcacccagaa tcgttgacaa gccggaaggg cgtccctata ctcagccgca tgtcgaagaa
     2761 ggaccggaac gagcctccct ccaccgcagc tgacgcgggt gtccgcctga aaccggtcct
     2821 tggggttcgt ccgggccagt acctcaccgt catctacgcc gtggtcctgg tcctggccgt
     2881 gtacctcctg ctgtttgttc ccggcctgcg ccaccggggc gcctatcttt ccctgcagag
     2941 cttcccggag cacgccgcgg tcacggtgga cggccggtac gcggggagca ccccgtgcac
     3001 ggtgtttctg ccccatggct cgcgcaccgt catggtctcg cggccgttct acacggccgt
     3061 cacgctgacg cgaaaggtcg ccggacgcgt gtttgcaacg ctgctcgtgc cggatcgctc
     3121 ccgcgcctcg gtatcgctcg acgtggcaga cgtgaatggt ctggccgccg gcgccctggc
     3181 cgacttccag aagaacccgc agattcccca gatcgtctcg caggccgcct gggccgccgc
     3241 tgccaacagc cgtgacccgt tgtacgacct tgtcgcccgg agccttctgg tgacgaccga
     3301 cgaatcgcag cttcgcgaga tccttgttgc ggcagggcgg gtctcggccc gtaccgggtt
     3361 tctcacggcc ggctccctgc tggacatggt ccagagggcc gcggcattcg cggagtccgc
     3421 ggacaacgcg ccagcctggc tgttgttgac gctgagccgc gccaaccaga cgcacatcgc
     3481 cgcaagcccg tgggtccagc agtacctgtc cggctaccgg gatgtcctct cccggtacta
     3541 ccagccgggc gcgccggtga gcgggcccgc gggcggcggg cctgttgtgg cgggcatcgc
     3601 attccgcgga atacccggag gcgacctcgt catgggcagg gacgatgagc tggacactct
     3661 gggcaagacg attgaccggc ttctcgccca ccccgtccgc gtggcccctt tcttccttgc
     3721 atcttctgaa gtgacgaacg cggagttcca ggccttcgtt gcagagaacc cggattgggc
     3781 gccgtccaac ggcgcggcgc tcatccagaa gggcgtcgtc acggacacgt acctggcgca
     3841 gtggaaatcg ggggcaccgg cagcgggaac ggccaacacg ccggtgacgt cagtgtcgtg
     3901 gtatgccgca gaggcgtact gccagtggct gacgcgccgg gtccaggcgt ccctgccggg
     3961 gtaccaggcc cggctgccct ccgaaagcga gtgggaatgg gcggcccggg gcggacttcg
     4021 agggatgccc taccccctgg gcgggaagcc cgggggtgcc gtgttcttct ccccggggat
     4081 cacaggcccg tcagcctccg gaacgtcgga gccaaacgga tacgggcttc gcgacatgct
     4141 tggcaacgtg tgggagtggt gctcggaccc gttcagcctg aacgcaagcg tgctctcgtc
     4201 ccgggacccg aaatccagcg cccagctcga ggcatccctg ccggatgctc cggaccgcgc
     4261 cgtgcgggga ggctcctggg cggatcagcc gggagctgac aaggtttaca cccgcggctc
     4321 ccagcccgcg cagtggtgca cgccgtacct gggattccgc gtggcgctgg ggcgtcgata
     4381 ggcgggaaag gcgcgccggc cgcgcgcgcg gtgagcgtga cgtcatggca aagtcagaag
     4441 gaatcaagat cctcgccgtc aacaagaagg cgaggttcaa ctactccatt gatgaaagct
     4501 tcgagtgcgg cgtcgtgctg gttggcaccg aggtgaagtc catgaaggag ggccgcttct
     4561 cgttcgcgga cgcctacgct tccgtggaga acgacgagct gtggctgcgg gacctgcacg
     4621 tgacgccgta cgcatttggc aaccgcatca accacgagcc gcttcgcaag cgaaagctgc
     4681 tggtgcatcg caaggagatc aagcgcctca agcggatcac ggatgagaag ggattgaccc
     4741 ttgttccact gaagttctat ctttcccggg gatacgtgaa gtgcgagctg ggcattgccc
     4801 ggggcaagaa gctctttgac aagcgtgacg acattcgaaa gagagaccag aagagggagc
     4861 tggagcgcga gttccgcgtg aagtagcgcg ccagatctcg gatccagcgg cgcgatgtgc
     4921 tcgccctctc gctgccccgc tgcgcgggtc agctgacggg cttcgcgcct cgcaaatagc
     4981 gagtcgctcg ggggtgcaac ggtctcgact ggggtcggtt cggccctggg ctgcaggtgg
     5041 agcgccagtc tcctaaaaat ggcaacacac aactgccaac aacgcagatt tcgctctcgc
     5101 cgcttaattg cgacgacggg tccctggagc gtggtaccga gcatccaggg gggcctgtaa
     5161 caaaccggga ctcacctgac tgcttgcctg tgagcggtta gggacactac agcaggcttt
     5221 ggcgatgaac gggacgccgc ctcccaccct cgtcgccgac gggctaacgg gcggataaac
     5281 ctgtagaggt tcttggtcaa cactccagga cgcgggttca actcccgcca cctccatcgc
     5341 gcggggcccg acgtgcgcgg gccccgttgg cgccgatgcc gtcacgtgcc cctagcttcc
     5401 tgtccagcac ttgccggacc caggcggtct gctccgcggg cgagtacggc ttgccgatga
     5461 agtcgatgcc gtcctccagg atcccgtggt gggccaccac tcctccagcc tctcctccgc
     5521 gctcccgagc cgccattctg cctggcgatt ccaaacaccg ttgccggccc aggcccgtgc
     5581 ctttcccttt cggcttggtg gagaagaaag gctcgaagac ccgcgccctg acctcctcac
     5641 tccattcccg tgccgctgtc ggacacaagg agcgccacgt actcaccgat ggctgcccgg
     5701 gtgctgttgc gagtagcgct catcgaggta cacgttcctg gtctccaccg tcaggtggcc
     5761 gtcgcccggc atggcgtccc gcgcgttgat cgccaggttc acaaggacct gttccgcgct
     5821 ctgccgcggc gcggtagtgc gccggtatct ccgtgtcggc cagcggcggg aatgcttgcg
     5881 ccagcgtctt tccccaccac cgtgccctga gagtcacgca gcggtctcga taacccggac
     5941 ctcgtcggcg cggaagcgcg ccgccccttc ggcgggatag aaagcactgt cctttctgcc
     6001 caccgcggcg tggtcggagc tcaagcacag caaggcggcg ttagcctcgt tcgcgatggg
     6061 gaagcggctt tccgtgtcct tcacctctgc ccgcgaacga gactgaccac cgccggatcc
     6121 tgcccgtccg ccggcagttc cagggcaccg cccccggccc tcaccgatcc actgttccat
     6181 ctgctgttcc agtgacgaac aaacgcgcgt tcgacgagtg tcgcaatgcc gcacgattac
     6241 atttgatatg tccattctct ttcccaagca actccaatat cagcatatga agtgacggct
     6301 cgagtcaccg gactcgtaaa taccgtttgg catgggaatt gttctcctat gcagcgaagc
     6361 aacccatttg aagcagtcgg aggtcaatat gctgaaaaac atcaaaattg gaacgaagct
     6421 ggtcaccgtc ggtgccctgt tgattgccat ccccctggca gttgtcgcgt tcctgtccgt
     6481 ccagcgcgca agcctggcgg tcatgtccat tgagtccgag cagctagcac gggcagcccg
     6541 gcttatcgcc acttctatca acaacgtgtt cctcgaggag cagaaggtcg ctctcaacaa
     6601 cgcaacggac aattcgatca tcgagacggc ggttgccgtg catgggtccg cggcgaacgc
     6661 cccgaagggc aagtcggcac ccagcgcggc ggacctgatc gcggtgtcta ccgcccggat
     6721 caaggccacg acggggctcg gtaaagacta cgaaagcatc atggtcgtcg ggaccgacgg
     6781 cgtggcgttc gcgtcaacgg accctgcgta cgtgggtgga aacttctcgg accgagcgta
     6841 catccacgag gcgctcgccg gcaaagccac ggcgggaacg gccgttatca gcaaggtcac
     6901 gaacaagccg gtcgtccccg ttgccgcgcc catcaaggac ggacggaacg tcgtgggcgc
     6961 gtacgtgctg gtcctggacg cgcagttcct gaacgatctc gtgaccggag agaaggtggg
     7021 caagaccggc tatgcctacg tcgtggacaa taccggcctg gtcatcgcac accctgttgc
     7081 cgccaacctc ttcaagacga acctggcgga gatggacggg acaaaggcct tcaccaagcg
     7141 catgatcgcc gg
//
LOCUS       DASRZS010000200         3321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_109980, whole genome shotgun sequence.
ACCESSION   DASRZS010000200 DASRZS010000000
VERSION     DASRZS010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109980"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..2981
                     /locus_tag="VFH83_04985"
     CDS             153..2981
                     /locus_tag="VFH83_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6485748.1"
                     /translation="MATSEPSPQGRWLRYSLLALLIAATAALTRLAWSDRGGLTASAF
                     QRRMPLKNPVSVAWDGKDHLYALDDRFFRILELTADGRVVRQFYAHSVDPRHYEYWEE
                     LAVDGDGVLYAAKVEYFIDSELIDSEQICRYPPGGAEQILYTVHHEGDTEAYDSRLLT
                     LQIKGGWLYFDVRAPDQVALWRLPLAGGNAEQVLVIPAKKEDVYNLAGTAPGQLWITS
                     YSGDRVWRLTTGGKLQDAAFQPAAPQVPPIVLADKVVTAPGGDLFITDLFNQCIYRVR
                     PGQGLELWLGKSDLPDHPERALFKDIWATPDGGLAAVDVIWRQTGARRPAAGEGGRLV
                     LFDPARRLLHEVTSPVPSLSLWLRLLQPWGAVFGAGLCFIGLLVFVYLVILGRRLSVM
                     LKLILAFVPLVVGSIAFVSTTTFSRSSAKVEEEIRYRLASLAQTGQLLINGDAVDRIR
                     RPADYLGEDYNAVAQQLQKLINSAKDPWNQRLFADVAKLYNGMFYIMDDSASTYGVLY
                     PLPVAPSQEYKAALQGTGPRTYEYTDTDGTFLEAAAPIRNHQGTAVAVLYVGSSKDDL
                     DLLQAEFRRGVIQSTALATALLLVAITGVSLFLLLSVNRLRGAVGRMAGGEWGAEVTI
                     RSRDEIGELGQGFNTMSRYLRGYISEITSMSDAYARFVPREFLRFLSKEKVADIELGN
                     QVEMDMAILFADIRSFTTISEKMRPQENFNFLNSYLSRMGPVVRRHRGFIDKYLGDGI
                     MALFPAGAESAVRAAVDMQRELVTYNRHRARSGYQPIAVGIGVHSGKVMLGILGEKER
                     MEGTVIADAVNLASRLEGLTKIYGAWIIVAEDVVKALGSRAPEHRFLGRVAVKGRSEP
                     VSICEVFAADGPRDIQLKRRTRKVFEEGVRLYLEGNLVEARRYFGTVHQVHPEDLVAA
                     YYASQSEGGNGTDAPRDAVSAEEATLVL"
     gene            2978..>3321
                     /locus_tag="VFH83_04990"
     CDS             2978..>3321
                     /locus_tag="VFH83_04990"
                     /inference="COORDINATES: protein motif:HMM:NF018685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HET6485749.1"
                     /translation="MKPRIFDILAVALSLGAIAAFSVYAYSGRTSSAEVVIEASGSQW
                     IYPLNVDRQEAVHGPLGDTIVVIRDGKASVQDSPCPDKLCVHMPAISRPGQWIACLPN
                     KVFVRVRGTSGE"
BASE COUNT          576 a         1052 c         1090 g          603 t
ORIGIN      
        1 gctgtcgggc acgggagttc ctctcttttc gggaatcgta gttcagcgaa cctaccgggt
       61 caagcccggc cgatcactcc ggtcacccct gcattcccgc cgcaagagaa aatggcggga
      121 ttggagaggc cggagcggta caatgaaggt acatggctac atcggaaccg tctccgcagg
      181 gccgttggct gcgatacagc ctcctggctc tcctcattgc cgcaaccgcg gcccttacgc
      241 ggctggcgtg gagcgaccgc gggggcctca ccgcctccgc gtttcaacgc cggatgcctc
      301 tcaagaaccc ggtgtccgtt gcctgggatg gcaaagacca cctgtacgcg ctggacgatc
      361 ggttcttccg catcctggag ctcacggcag acgggagggt ggtgcggcag ttctacgccc
      421 actccgtcga tcccaggcac tatgagtact gggaggagct cgcggtggat ggagacggcg
      481 tgctctacgc ggccaaggtc gaatacttca tcgacagcga gctcatcgac tcggagcaga
      541 tctgccgcta ccctccgggc ggcgccgaac agattctcta caccgtgcat cacgagggag
      601 acaccgaagc ttacgactcg cgtcttctca cgcttcagat caagggcggc tggctgtact
      661 ttgacgttcg ggcaccggac caggtggccc tgtggcgcct ccccctggcc ggaggcaacg
      721 ccgaacaggt cctcgtgatt cccgcaaaga aagaggatgt gtacaacctc gcggggacgg
      781 ctccaggtca gctatggatc acgagctaca gcggtgaccg cgtgtggcgc ctcaccaccg
      841 gcggcaagct ccaggacgcc gccttccagc cggcagcccc ccaggtgccg cccatcgttc
      901 tggcggacaa ggtagtgacg gctcctggcg gtgacctttt catcacggac ctgttcaacc
      961 agtgcatcta ccgagtccgg cccggccagg gcctcgagct ctggctcggc aagagcgacc
     1021 ttccggacca tcctgagagg gcgctcttca aggacatctg ggcgacgccg gatgggggcc
     1081 ttgccgccgt ggacgtgatc tggcggcaga caggagcccg ccgtcccgcg gccggcgaag
     1141 ggggccgcct cgtcctgttc gatcccgcgc gacggctgct gcacgaggtg acctcccccg
     1201 tgccatcact ctccctgtgg ctgaggctcc tccagccctg gggcgcggtc tttggggccg
     1261 ggctgtgctt catcggtctc ctggtctttg tctatctcgt catcctgggc cgcaggctca
     1321 gcgtcatgct gaaactgatc ctggccttcg ttcctctggt ggtgggctcc atagcctttg
     1381 tttccaccac taccttcagc cgttcctccg cgaaggtgga ggaagagatc cggtaccggt
     1441 tggcttccct ggcccagacc ggccagctcc tgatcaacgg ggacgccgtg gaccggatcc
     1501 gtcgtcccgc cgattacctg ggggaggact acaacgcggt agcccagcag ctccagaagc
     1561 tgatcaactc ggccaaggat ccgtggaacc aacggctgtt tgccgatgtc gccaagctgt
     1621 acaacgggat gttctacatc atggacgatt ccgcctcgac ctacggggtc ctgtatcccc
     1681 tcccggtggc gccttcgcag gagtacaagg ccgcccttca aggcaccggg cctcgaacct
     1741 acgagtacac cgacacggac ggaacgttcc tggaggcggc ggcgccgatc cgcaatcacc
     1801 aggggaccgc cgttgccgtc ctctacgtgg gatcctcaaa ggatgatctg gacctcctgc
     1861 aggcggagtt ccgacgggga gtgatccaga gcaccgccct ggccacggcg ctgctcctgg
     1921 tggccatcac gggtgtgtcc ctgttcctgc tgttgtcggt caatcgtctg cgcggggccg
     1981 tagggcggat ggcgggggga gaatggggag ccgaggtcac cattcgttcc cgggacgaga
     2041 tcggcgagct tggccagggt ttcaacacca tgagccggta cctccgcggc tacatcagcg
     2101 agatcacgtc gatgagcgac gcatacgctc ggttcgtccc gcgggagttc ctgaggttcc
     2161 tttccaagga gaaggttgcc gacatcgagc tgggaaacca ggttgagatg gacatggcca
     2221 tcctcttcgc cgacatccgg tccttcacca cgatctccga gaagatgcgg ccccaggaga
     2281 acttcaactt cctgaactcg tacctgagcc ggatgggccc cgtggttcgg cgccatcggg
     2341 ggttcataga caagtacctg ggagatggta tcatggccct gttcccggcg ggcgctgaga
     2401 gcgccgtgag ggccgcggtg gacatgcagc gggagttggt aacgtacaac cgccatcgag
     2461 cccgaagcgg gtaccagccc attgcggtgg gcatcggcgt ccattcgggg aaggtcatgc
     2521 tggggatcct gggcgagaag gagcggatgg aaggcacggt gatcgcggac gccgtgaacc
     2581 tggcctcccg cctggaaggt ttgaccaaga tctacggggc atggatcatc gtggcggaag
     2641 acgtagtgaa agcccttgga tcccgggcgc cggagcacag gttcctgggt cgggtagcgg
     2701 tgaagggcag gagcgagccg gtgtccatct gcgaggtctt cgcggccgac ggtccccggg
     2761 acatccagct caagcggcga acgcgaaagg tcttcgagga gggagttcgg ttgtacctgg
     2821 aaggcaacct ggtggaagcg aggcggtact tcggcaccgt gcatcaggtc cacccggagg
     2881 atctcgtggc ggcctattat gcctcccaga gcgagggcgg gaacggaacc gacgccccac
     2941 gggacgccgt cagcgccgag gaggcgaccc tggtcctgtg aagccccgga tcttcgatat
     3001 cctggcggtg gccctctccc tcggtgccat cgccgccttc tccgtatatg cgtactcggg
     3061 tcggaccagc tcggccgagg tggtcattga ggcctccggc tcccagtgga tctacccgct
     3121 gaacgtggac cgccaggaag cggtccacgg cccgttgggg gataccatcg tggtgatccg
     3181 ggacggcaag gcgtctgtcc aggattcacc gtgcccggac aagctgtgcg tgcacatgcc
     3241 cgccatctcc cgacccggcc agtggatcgc gtgtctgcca aacaaggtgt tcgttcgcgt
     3301 caggggaacc agtggcgagc a
//
LOCUS       DASRZS010000201        18155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1644246, whole genome shotgun sequence.
ACCESSION   DASRZS010000201 DASRZS010000000
VERSION     DASRZS010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 18155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18155
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644246"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VFH83_04995"
     CDS             <1..868
                     /locus_tag="VFH83_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485750.1"
                     /translation="TLTLEPYRSPRRRRPARTVVYQTVIQILLAIGAVVMIFPFYFMI
                     TSSFKAENRINLVPPEWWPTHWITDNYAQLFTMIDVTRLFMNSLVVAAVVTAVTMYTS
                     GIVGYLFAKFQFKRKEVYFLVILATMMIPWPVTMVPVYQICLKLGMINTYWGIIVPYL
                     FSTFGIFMIRQFMYHIPTDLLDAARIDGAGEFQLYHRVVIPNAMASISALGIFTFLWN
                     WDNFLWPMITLMDERLFTLPLGIAMFAGRWWTQLGPVLSGATVSVVPVLIVFLIFQKN
                     ITEGLTMTGIKG"
     gene            complement(927..1754)
                     /locus_tag="VFH83_05000"
     CDS             complement(927..1754)
                     /locus_tag="VFH83_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET6485751.1"
                     /translation="MADTPLRVMVIGAHPDDPDICCGGSAIQWAARGHVVKFLSVTNG
                     DTGHHAIGGIELARRRYHEAQASARVAGIQEYQILDNHCGELEPTVANRKVVIRLMRE
                     FAPDLVITHRPNDYHPDHRYTSQLVQDASYIITVPNMVSLTDILKAPPLICYVYDTFQ
                     RPYPFHPDVVVGTDTVIEKKLDMLDCHVSQMYEWLPFNRGVLDQVPSDPRERRKWLPA
                     QYMARFEEVAAACRAGLIQAYGGKRGESMRTAEAYEFCEYGARPTPELVRRAFPLFD"
     gene            complement(1837..2661)
                     /locus_tag="VFH83_05005"
     CDS             complement(1837..2661)
                     /locus_tag="VFH83_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485752.1"
                     /translation="MRSRIGPPVRFLSLALLSVLMLTPLAWALAASFRPPEIPFASGS
                     VFFGSSLSTANYAKALSLAPFARYYLNTILQVLAIIGVQVGTASLAAFAFARYRFRGE
                     RALFTVILLQMMIPTAALLVPNFATIRFLKLYDTIPGIALPFFGSAFATFLLRQTFLS
                     LPRDLADAASIDGCRWDQELRHVYLSTSRASLAAIVLSSLSYHWNDFLWPLVITQSDR
                     ARPLTAGLVRFTQMGEIGAQWGLLSAATMIVTLPLFVAFVVFQRQFVRGYVSSGIK"
     gene            complement(2651..3493)
                     /locus_tag="VFH83_05010"
     CDS             complement(2651..3493)
                     /locus_tag="VFH83_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485753.1"
                     /translation="MAPTLVFVLVFTVLPLVESIVGSLYKHKLNIPRYQVPVFSGLTN
                     FSDLFRDPEFLRVVTNTGFYALILIPVLLVAALGLALLLESRIRGLPFFRLAVFHPTV
                     LPLVSAATIWMFLFTPGYGLWNRLLQLFGYTGPQNWTANPSLALPAVALVAFWKNVGF
                     LMLFFLAGLQNLDRSLLEAARLDGAGGLRLLTGIILPLIRRQTLFVTTVAFIGAFQTV
                     DHVFVLTQGGPSGMSTLPLYYLWQLRFDRMDIGRGDAVTVLLIAALLAFTVTNTLLSE
                     RHEK"
     gene            complement(3546..4829)
                     /locus_tag="VFH83_05015"
     CDS             complement(3546..4829)
                     /locus_tag="VFH83_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6485754.1"
                     /translation="MRKLALVTAIFAVFFGSALAPSQVVAAPVEVSLAYPVAVDAPIT
                     AMLKGWAQDYMAKNPDVKVTLIFSGGYPDVKTAVQTAIQGGAKPPTLAVMLATDVYDL
                     VNARYVDSLDDLVAQAPGGAAFVKDFLPAYMGNSQYKGKLWSLPFQRSAVVLYYNADL
                     FAAAKLSPPRSWDEMGRDAQALTVDGGKTRWGIEIPTENPYWTFQPLAIGSGKNVFSD
                     DVTVNFNAPEVVDAVQFYLDLSQKYRSTPAGVQASWGTSTQNFAAGKTAMIIHTTGSL
                     AGILKTAKFKVGVMAVPGKLPGTLASVTGGGNVYLTAGHTPEERKAAFALIQYILDPS
                     RVATFSQATGYIPHRASATATAEYKAWLEKVPQAADAVAALASAQPELATQNLNGVKT
                     IFNNYLQVAFNGGKSPKDAMDAAENESNRVLADYR"
     gene            complement(4889..5761)
                     /locus_tag="VFH83_05020"
     CDS             complement(4889..5761)
                     /locus_tag="VFH83_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6485755.1"
                     /translation="MNRVGIYYAYWTHDWDADFVPFVTRVKRLGFDALEVNSGTVAGM
                     TRSHRERLRSAAQDEGVALSSCIGLPAKFDPSSADLSVRRRGVRFLAKQIEAAAATGI
                     SAISGILYGAWPAAMPAGETDKRPYWDRSVKSMREACRVAADRGVCLNMEVVNRFEQF
                     LLNTAVEAVRYATEVGSPACRILLDTFHMNIEEDGFRSAIRAAGPRLGHVHLGETNRR
                     PPGRGKMPWGEIFGALRSIGYRGSLVMEPFLMPGGEVGRDIRVYRDLRDGKDLDREAA
                     RACRFVRAGLSRQN"
     gene            complement(5762..6685)
                     /locus_tag="VFH83_05025"
     CDS             complement(5762..6685)
                     /locus_tag="VFH83_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017277490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET6485756.1"
                     /translation="MYFIGVSTAGSMIMKVFPVWSRILGLGDCELRGIDLALHDDPRS
                     YRAVVEFIRDDPLSLGALVTTHKIDLLHAARDLFDELDGFAALMGEVSAIAKSGGRLT
                     GAAKDPISSGLALRAFVPAGYWEKNGACALLLGAGGSSIALSSYLADRARGADRPARI
                     FVTNRSAGRLDAIQAIHRAQSVDIPVRYELTPRPEDNDAVLSQLPPGSLVANATGLGK
                     DGPGSPITDGALFPTDAFAWDFNYRGELAFLRQARAQEASRRLRVEDGWLYFVYGWLS
                     VIAEVFHRDIPLRGPEFDRLRAAADQERPRA"
     gene            complement(6727..7317)
                     /locus_tag="VFH83_05030"
     CDS             complement(6727..7317)
                     /locus_tag="VFH83_05030"
                     /inference="COORDINATES: protein motif:HMM:NF018288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HET6485757.1"
                     /translation="MSATESRRGPDLPFGVDFDLVQGIMKEPSSHVVRRASGMRGYYR
                     DARALEALIAQGDPVHYETFERAVTEKRGNLMFCISRTNAGTVGQECFMTKGHYHAVP
                     ETAEIYLCLRGDGWMVMKLPDGTPARERLTVGRLVYVPPFWAHRTVNTGDEALVSLCI
                     YPAEAGHNYGDIEKEGFPQRVYRRNGKVEFVSETRE"
     gene            complement(7314..8849)
                     /locus_tag="VFH83_05035"
     CDS             complement(7314..8849)
                     /locus_tag="VFH83_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET6485758.1"
                     /translation="MTYLGVDIGTQGVKAGLYDAEGRCRAEAFRKSELHQPAAGVVEE
                     DPEKQLASVCECLAECVGRAAAVGGAPGIAVDGIAIDGQMAGIIGVGADGRCVTPYDS
                     WLDTRCAPWITHMRREAGAEIVRKTGGPASFNHGPKVLWWKHERPESFSRIRAFVQPG
                     GYAAMRLCGMDASHAFIDASYLHFSGFADASGPRWDPELCRVFGVDPGLLPRIVLSHE
                     VIGGLSRGMAAACGLPPGVPIVAGCGDTAASFLSCGAAQAGVCADVAGTASVFAATTS
                     AFRPDVEEQILCCGRSATPGLWHPYAYVAGGGMNLEWFRERFAGDVEGGLDRLNDLAA
                     AVEPDADDPMFIPHLGGRVSPSQPDLRGAWVGLAWGHGPGHLYRAVLEGVALEYGLYL
                     RRLESLFGAGTVRQVRVTGGGEKSVVWNRIKADTLGVPVVQVAGAGGAPRGAALLAAY
                     GVGAVADLPAAAARWVATGTVTEPDARRVPVARRRIDRYAELLRMMNRWSEAGRGAGR
                     EKT"
     gene            complement(8934..10049)
                     /locus_tag="VFH83_05040"
     CDS             complement(8934..10049)
                     /locus_tag="VFH83_05040"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET6485759.1"
                     /translation="MSEQRTPMAHVRESLKVAGYRCPIPPDRLRELTRRSDAQGALQT
                     IGFLALVVALGLVTWHLYRQRLWGWMALALFAYGTVFSFTPGPHELLHGTVFRTKWLN
                     GLFHRIYCMIQWHNPHEYKRSHTYHHVYTLHPEGDREVVLPITPSLHPLRILELLTFD
                     VTAFWIVLSRTVTLAFTGRFHNEWSGAVFADDATARRLAVRWARLQLLFHALVIAASI
                     VLRLWLLPVLVTIGQYVANLWMYLVLMPMHTGLRSNVTDFRKCVRSITLDPFTEFLYW
                     HMNWHTEHHMYAAIPCYNLKRMSRAIAADMPRPRTLIGAWVEMRATYQRQKRDPTFEF
                     DTPVPPPRAKAPDPKDPLRSAIGDLAPKGLEGVGRSA"
     gene            complement(10128..12494)
                     /locus_tag="VFH83_05045"
     CDS             complement(10128..12494)
                     /locus_tag="VFH83_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET6485760.1"
                     /translation="MSDPLYRDASRSPKARAADLLARMTPAEKTAQLGSCWSFELLSG
                     FTFSSEKAEARLANGIGQVTRLGGATNLKPDRCAALANQIQRHLREHTRLGVPAIVHE
                     EICSGLMSWGATLFPQAIGVAAGWNPELVERMAAAIRGQMRAIGAHQGLAPLLDVCRD
                     PRWGRTEETFGEDPYLVSLLGCAYVRGIQGGDAPDGVVATAKHFVGYGGAEGGMNWAP
                     AKIPPRELAEVYLAPFEAAVRMARLGSVMNSYGEIDGVPCGASRWLMSQLLRDEWGFD
                     GIVVSDYWAIPMLERYHHVARSGAEAAGFALRAGIDVDLPSTSSYGESLTQALDAGLV
                     GMADVDRAALRVLTAKFRLGIFERPFVPEGRVLELFDTPAQNECAARLARQSIVLLSN
                     NGILPLSPDTRSIALIGPSADDWRNLIGDYAYPCHVEVLAATKEDNPFGMPIPDDLGD
                     VSRSLRIPTVLEALREVSDGGVRITHARGCDVKTEREDGIPEAVRLARESQVAILVLG
                     DRAGLTTECTSGESRDCADLRLPGVQERLLEAILQTGTPVIVVLAVGRPYALDAFSDR
                     VAALLCAWLPGQAGGGAVADALVGRFSPSGKLPISFPRSAGQVPVYNYHTPSGGRSHW
                     TGDYVDRPASPLYPFGHGLSYATFAYKDLCVSPGEVTGRGTARVSFSIRNMGTRAAAE
                     IAQLYVGYRPEGTPVTRPVRQLKGFHRVVLEPGQEVNVEIELATDELAFPDLDMKLRL
                     YPGSVDVLVGSSSEDIRLKGSLRIQGPSTDIDPRTRRLFAESREVARG"
     gene            complement(12491..13489)
                     /locus_tag="VFH83_05050"
     CDS             complement(12491..13489)
                     /locus_tag="VFH83_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485761.1"
                     /translation="MSDNALLRADHLTRVYTLGRIFAGQKIVAVNDASFVLPADSPQV
                     FTLAGESGSGKTTIAKMILGFVEPSQGSISYKGADITRIRGRAQRMQFMREIQPVFQN
                     PFETFNPLKKVENYLFETAVNFGMAPSRSAARRPVQAALERVGLNLDAVLGKYPNEFS
                     GGQLQRISVARSLITNPSVLIADEPVSMVDASLRMSIVNLFKELRAEHRVSVVYITHD
                     LATAYYVSDRIAIMLRGTIVEIGDVEKVLDHPEHPYTRILKESVPEPDPALKWNQDIA
                     MADGEQAEYRLAACKFAGRCPHVMEVCRAVEPPLAEHDGRLVRCHLYSGGVTGARA"
     gene            complement(13479..14477)
                     /locus_tag="VFH83_05055"
     CDS             complement(13479..14477)
                     /locus_tag="VFH83_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485762.1"
                     /translation="MPVLKVENLRAHYLTRLYGLQSHVKAVDGITLDVEENEIYGIAG
                     ESGCGKTTLIKTLTGLVQPPLTIMGGSVRYTLENREIDILSLRRESEARQVRGRLVSY
                     IPQGSMSVLNPVRKIRKSFWDFMSAHRRFAGRGEFQDLVEAHLRSLGLSPAVLKAFPH
                     QLSGGMRQRVTIALATILRPRLILADEPSTALDVIMQRGVIQLLKRIREEMQNTVILV
                     THDMAIHANICDRVAIMYAGEIVEEAPVSDLFKGARHPYTRFLLRSLPSIGDKTPRVS
                     APGAPPSLTDPPSGCRFHPRCPEAKELCHVRNPRLVRIGPRHRVACFVDEDTRYER"
     gene            complement(14481..15344)
                     /locus_tag="VFH83_05060"
     CDS             complement(14481..15344)
                     /locus_tag="VFH83_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485763.1"
                     /translation="MRTVGNLLRHDGRFRFGFVVTAALVALAILSLFIPWDPKLWNVF
                     PRDRPISWTNPLGTNSMGQDIFWQTTVAIRNSLILGLVAAAISRVIALVIGLVAGYRG
                     GILDRILMTVNDSFVIIPIFPLLILVSAILKGHMNVTILGFVLGLFGWQWDARLFRSM
                     VLTLREREFTNTAVLSGMKAVRIVFSEHLPFVVPLVMAASLNNILWAIGMEVTLAVLG
                     LSSLEIPTLGTMIYWAMQYQAIFLGIWNWILTPVVLCVLLILALYLLSVSISEYLDPR
                     TRLMRIRLREE"
     gene            complement(15362..16369)
                     /locus_tag="VFH83_05065"
     CDS             complement(15362..16369)
                     /locus_tag="VFH83_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485764.1"
                     /translation="MQFLKRYLLPRLVQFVVVIFIGATVVFIIPRLTPNDPIERQLAN
                     LTTRGAYLDSATIGKIKQTLRELYGLEGTFLQQYGLFWERLFHGDFGPSFSHFPTPVT
                     TLIRNSMPWTIGLMLITTFLSWAIGSLLGGLAGSFRRAKWARAIEIFSMTIRPVPYYI
                     MALALVILFAYVFPVFPMIGGYSVKLKGAVTLKYVLDVVRHAFLPAISLVLTGFGTWF
                     LQMRSVTFTVVAEDYVTYAEAAGVPRGKIVTQYITRNAILPQVTGLALSLGFIFGGAL
                     ITEQVFAYPGIGTLLYNAVISSDYNVIMGITVYSIVSIAVAVLVIDLIYPLLDPRVRH
                     R"
     gene            complement(16434..>18155)
                     /locus_tag="VFH83_05070"
     CDS             complement(16434..>18155)
                     /locus_tag="VFH83_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6485765.1"
                     /translation="AGWVGNDKGMQQLVADPLWTVEYTLGQNVPTLASDPPIYNKDFT
                     RMTAKLRKGVTWSDGVPFTADDVVYTVQVQMANKGMTYYAQMTDNVAKVSKTDDYTVV
                     FDLKKPNAKFDVFFNDRWGALRILPKHIWEKQADPMTFQFYPPVGTGPYVLKDYDTDG
                     YWFTYERRADWDKSPAGMLYGKPAPTYVQFVYYGDPDKKVIAESQHQLDMCDLTPEAF
                     VAAQKKVPTVRGLYKDFPWYEILHPCTTGGVFNCQVAPYNDADVRWALNLALNIPQMV
                     MTAYNGATALAPAYVPATLPFYKWYYQPMQQWLKDFTITVGGKPYKPYDPDMPNTLAR
                     QAKAAGYPVPTTQAGINEMFGYGGWKYDPAAAETLLKGKGFSRDKNGKWLLPDGKPWS
                     IHINAPTNPAHPSFKWGFQLGDQWRKFGIDVNVDANEQSSTVDSNGSFDVSGAWPMWA
                     NGGDWDLFSVMQQYHSKYLVPLGTALAGGETARFSTPELDKIIDQMEQVPSLDPKSQQ
                     LSLAGLKVLVKYQPGISLGSYVSVLGWDEYYWTNYPGGGNQYTQPHYHWPNFKFMLPR
                     LKPTGKQ"
BASE COUNT         3178 a         5971 c         6032 g         2974 t
ORIGIN      
        1 caccctgacc ctggagccct accgctcccc cagacgccgc cgccccgcgc ggaccgtggt
       61 ctaccagacc gtcatccaga tcctgctggc catcggcgcc gtggtgatga tcttcccgtt
      121 ctacttcatg atcacctcct ccttcaaggc ggagaaccgg atcaacctgg tcccgccgga
      181 gtggtggccc acccactgga tcacggacaa ctacgcccag ctcttcacga tgatcgatgt
      241 gacccgcctc ttcatgaaca gcctggtggt ggcggccgtc gtcaccgcgg tcacgatgta
      301 cacgagcggg atcgtgggtt acctcttcgc caagttccag ttcaagcgca aggaggtcta
      361 cttcctcgtt atccttgcca ccatgatgat cccgtggccg gtgaccatgg tgcccgtcta
      421 ccagatctgc ctgaagctgg ggatgatcaa cacctattgg gggatcatcg tcccgtacct
      481 cttcagcacc ttcgggatct tcatgatccg ccagttcatg tatcacatcc cgactgacct
      541 cctggacgcg gcgcggatcg acggagccgg ggagtttcag ctctaccacc gcgtggtgat
      601 ccccaacgcc atggcctcca tctccgcgct tgggatcttc acgttcctct ggaactggga
      661 caacttcctc tggcccatga tcacgctcat ggacgagcgg ctcttcacgc ttcccctcgg
      721 gatagccatg ttcgccggga gatggtggac ccaactgggc ccggtgctgt ccggcgccac
      781 ggtgagtgtg gtgcctgtgc tgatcgtctt cctgatcttc cagaagaaca tcacggaagg
      841 tctgacgatg acggggatca agggctgacc cccgggcgcg ggcggcccgc gccgccccgc
      901 ggcggaccgc ctcgccccgc agccgctcag tcgaagagcg ggaacgccct gcggaccagc
      961 tccggcgtcg gccgcgcgcc gtattcacag aactcgtacg cctccgcggt ccgcatggac
     1021 tccccccgtt tcccgccgta tgcctggatg agtcccgcgc ggcaggccgc cgccacctcc
     1081 tcgaacctcg ccatgtactg cgcgggaagc cacttccgcc gctcccgcgg gtcggacggc
     1141 acctgatcca gaacgcctcg gttgaagggc agccactcgt acatctgcga cacgtggcag
     1201 tccagcatgt ccagcttctt ctcgatcacg gtgtccgtgc cgaccaccac gtcgggatgg
     1261 aacgggtacg gccgctggaa ggtgtcgtac acgtagcaga tgagcggagg cgccttgagg
     1321 atgtccgtga gggacaccat gttcggaacc gtgatgatgt aggacgcatc ctgaacgagc
     1381 tgcgacgtgt accgatgatc ggggtggtag tcgttcggcc gatgggtgat cacgaggtcc
     1441 ggcgcgaact cccgcatcag ccggatcacg accttgcggt tggcgacggt tggctccagc
     1501 tccccgcaat gattgtccag gatctggtac tcctggatgc cggcgacccg ggcggaggcc
     1561 tgcgcctcgt ggtaccgccg gcgggcaagc tcgatcccgc cgatggcgtg atggccggtg
     1621 tcgccgttgg taacggacag gaacttcacc acgtggccgc gcgcggccca ctggatggcg
     1681 gaaccgccgc aacagatgtc cggatcgtcc ggatgggcgc cgatgaccat aacgcgaagg
     1741 ggtgtgtctg ccatggtgcc cggagtatga cacgccgggc cggtggatca agccgcgaag
     1801 atcggccccg ccggtccgcc ctcgagggcc gctcccctac ttgatccccg aagacacgta
     1861 cccccgcacg aactgcctct ggaacacgac aaacgccacg aacaggggga gcgtgacgat
     1921 catggtggcc gcggagagga gaccccactg agccccgatt tctcccatct gcgtgaaccg
     1981 gaccaggccc gccgtcagag ggcgggcgcg atcggactgg gtgatcacca ggggccacag
     2041 gaagtcgttc cagtggtagg agagggagga caggacgatg gcggcgagcg acgcgcggga
     2101 ggtggagagg tagacgtgcc tcagctcctg gtcccagcga cacccgtcga tggacgcggc
     2161 atccgcgagg tcccgcggca gcgagaggaa ggtctggcgc agcaggaacg tggcgaacgc
     2221 cgacccgaag aacgggaggg cgattccggg gatggtgtcg tagagcttga ggaaccggat
     2281 ggtcgcgaag ttcggcacca ggagcgccgc cgtagggatc atcatctgga gaaggatgac
     2341 ggtgaagagc gcccgctccc cccggaaccg ataccgcgcg aaggcgaagg ccgccaggct
     2401 ggccgtgccc acctgtaccc caatgatggc cagcacctgg aggatcgtgt tgaggtagta
     2461 ccgggcaaag ggcgccagcg agagggcctt ggcgtagttg gccgtgctca gcgaggagcc
     2521 gaagaagacg ctgccggaag cgaaggggat ctccggcggt cgaaacgacg cggcgagcgc
     2581 ccacgccagc ggcgtgagca tgaggacgct cagaagcgcc agggagagaa accgtaccgg
     2641 cggtccgatc ctacttctca tggcgctcgc tcagcagcgt gttcgtgacc gtgaaggcca
     2701 ggagcgccgc gatcagcagc accgtcaccg cgtctcctcg gccgatgtcc attcggtcga
     2761 accggagctg ccagagatag tacagcggca gcgtgctcat cccgctgggt ccgccctggg
     2821 tcaagacaaa tacgtggtcc acggtctgga acgccccgat gaacgccacg gtcgtcacga
     2881 acaaggtctg gcgccggatc agcggaagga tgatcccggt caggagccga agaccaccgg
     2941 caccgtccaa ccgtgccgcc tccagcagcg agcggtccag gttctggaga cccgccagaa
     3001 agaagagcat gaggaacccg acgttcttcc agaacgccac cagcgccacg gccggcagag
     3061 ccagggacgg atttgccgtc cagttctggg gacccgtgta cccgaagagc tggaggagcc
     3121 tgttccacag cccgtagccc ggcgtgaaga ggaacatcca gatggtggcg gcgctcacca
     3181 ggggcaggac cgtgggatgg aagacggcca ggcggaagaa tggcaggccc cggatcctgc
     3241 tctccagcag caaggccagg ccgagcgcgg ctaccagcag gacggggatg aggatcagcg
     3301 cgtagaatcc cgtgttggtc accaccctca ggaactcggg gtcgcggaac agatcggaga
     3361 agttggtcag acccgaaaac acgggcacct ggtaccgggg gatgttcagc ttgtgcttgt
     3421 agaggctgcc gacgatggac tcgacgagcg ggagtacggt gaagacgagg acaaagacca
     3481 gggtcggcgc cacgagcgca tagggaagga tccggtgtcg cttcgtggcg ccagcccctg
     3541 ggcgcctagc ggtaatccgc caggacccgg ttggattcgt tctcggcggc gtccatcgcg
     3601 tccttcggag acttgccgcc gttgaaggcc acctgcaggt agttgttgaa gatggtcttc
     3661 acgccgttca gattctgggt agccagctca ggctgggcgg atgccagcgc ggccacggcg
     3721 tccgcggcct gcggcacctt ctccaaccat gccttgtact cagccgtggc cgtggcggag
     3781 gcgcgatgcg gaatgtagcc ggtcgcctga ctgaaggtgg cgacccgaga cggatccagg
     3841 atgtattgga tgagcgcaaa cgccgccttg cgctcctctg gagtgtgtcc ggcggtaagg
     3901 tagacgttcc cgccaccagt gacgctggcc agggtccccg gaagcttgcc gggcactgcc
     3961 atcaccccga ccttgaactt cgcggtcttc aggattcccg cgaggctccc ggtggtgtgg
     4021 atgatcatgg cggtcttccc ggccgcgaag ttctgggtgg atgtccccca gcttgcctga
     4081 acgcccgcgg gagtggaccg gtacttctgg ctcaggtcca ggtagaactg caccgcatcg
     4141 acgacctcgg gcgcgttgaa gttcaccgtc acgtcatcgg agaacacgtt cttgccggag
     4201 ccgatggcga gcggttggaa ggtccagtac ggattctccg tggggatctc gatcccccag
     4261 cgggtcttcc cgccgtccac cgtcagggct tgcgcgtccc ttcccatctc gtcccagctc
     4321 ctgggcgggg acagctttgc ggccgcgaag aggtccgcgt tgtagtacag gaccaccgcc
     4381 gatctctgga acgggaggct ccagagcttc cccttgtact gggagttgcc catgtaggcc
     4441 ggcaggaagt ccttcacgaa cgccgccccg ccgggggcct gcgccaccag gtcgtccagg
     4501 ctgtcgacgt atcgagcgtt gacgagatcg tagacgtccg tagccagcat cacggccagc
     4561 gttggcggct tggcgcctcc ctggatcgcg gtctggaccg ccgtcttcac gtccgggtac
     4621 ccaccggaga agatcagcgt gaccttcacg tccgggttct ttgccatgta gtcctgggcc
     4681 cagcccttga gcatcgcggt gatcggcgcg tcgacggcca ccggatacgc gaggctgacc
     4741 tccacgggcg cggccaccac ctgggatggc gccaaggcgg atccaaagaa gaccgcgaag
     4801 atcgcggtca cgagcgcaag ctttcgcata ccccctcctt cgatgtcctc agacgtcggg
     4861 gaagcatcgc ccggaatcgc tgtccaagtc aattctggcg gctcaatccc gcccggacga
     4921 acctgcaggc gcgggcagcc tctcgatcca gatcctttcc gtccctcaga tcccggtaga
     4981 cccggatgtc ccgacccacc tccccgcccg gcatgagaaa cggttccatg acgagggagc
     5041 cacggtaacc gatggagcga agcgcgccga agatctcacc ccagggcatc ttcccccggc
     5101 cgggaggtcg gcggttcgtc tcccccaggt gcacgtggcc cagcctcggg ccggccgcgc
     5161 ggatggcgga ccgaaagcca tcctcctcga tgttcatgtg gaacgtgtcc aggaggatcc
     5221 gacaggcggg gctgcccacc tccgtcgcgt accgcactgc ctcgaccgcg gtgttgagca
     5281 ggaactgctc gaagcggttg accacctcca tgttcagaca taccccgcga tccgcggcga
     5341 cccgacacgc ttcccgcatg ctcttgacgc ttcggtccca gtagggccgc ttatccgtct
     5401 cccccgccgg catcgcggcc ggccaggcac cgtagaggat cccgctgatg gcagagatgc
     5461 ccgtcgccgc ggccgcctcg atctgcttcg ccaggaaccg cacgccgcgg cgacggacgg
     5521 aaaggtccgc cgaggatgga tcgaacttcg ccggaagccc gatgcaggag ctgagcgcca
     5581 ctccctcatc ctgggcggcg gaccgcagcc gctcccgatg agagcgggtc atccccgcaa
     5641 ccgtcccgct gttcacctcc agcgcgtcga agccgagcct cttcacccgt gtcacgaacg
     5701 gaacgaagtc cgcgtcccag tcgtgcgtcc agtacgcgta gtagattccg actcggttca
     5761 tctaggccct cgggcgctcc tggtccgccg cggcgcgaag ccgatcgaac tccggtccac
     5821 gcaacgggat gtccctgtgg aacacctccg cgatcacgga caaccagccg tagacgaagt
     5881 acaaccagcc atcctccacg cgcagccggc gggatgcctc ctgggctcgc gcctggcgca
     5941 gaaaggccag ctccccgcga tagttgaagt cccaggcgaa cgcatcggtc ggaaagagag
     6001 cgccatccgt gatcggggag cccgggccgt ccttccccag acccgtcgcg ttcgccacga
     6061 gggagccggg aggaagctgg ctcagcaccg cgtcgttgtc ctcgggtcgg ggcgtcagct
     6121 cgtagcgtac cgggatgtcg acggactgcg cccggtggat ggcctggatg gcatcgaggc
     6181 ggcccgcgct tcggttggtg acgaatatcc gcgccggccg gtctgcgccg cgagcccgat
     6241 cggcaagata ggagctcaac gcgatgctcg agcccccggc tcccaggagg agggcgcacg
     6301 ccccgttctt ctcccagtag ccagccggca caaaggcccg cagggccagg ccgctggaga
     6361 tgggatcctt ggcggctccc gtcaggcgcc caccgctctt ggcgatggcg ctcacctccc
     6421 ccatcagcgc ggcgaagcca tccagctcgt cgaacaggtc gcgggcggcg tgcagcagat
     6481 cgatcttgtg ggtcgtcacg agcgcgccga gggacagggg atcatcgcgg atgaactcca
     6541 cgaccgcccg atagctccgc gggtcgtcgt ggagcgccag atcgatcccc cgaagctcgc
     6601 agtcccccag ccccaggatc ctgctccaga ccgggaacac cttcatgatc atcgacccag
     6661 cggtgctcac gccgatgaag tacatggtgg gccgtcgcgc cggctggagg gaaggcgggc
     6721 gggtggtcac tcccgggtct ccgagacgaa ctccaccttg ccgtttcttc ggtagacgcg
     6781 ctgcgggaac ccctccttct cgatgtcgcc gtagttgtgg ccggcttcgg ccgggtagat
     6841 gcagaggctc accagcgcct cgtcccccgt gttgacggtg cggtgcgccc agaacggggg
     6901 cacgtacacc agccgtccga ccgtcagcct ctcccgcgcg ggtgtgccgt ccggcagctt
     6961 catcaccatc cagccgtcac cgcgaagaca caggtagatc tccgccgttt cgggcaccgc
     7021 gtggtagtgg cccttggtca tgaagcattc ctgtcccacc gtccccgcgt tggtgcggct
     7081 gatgcagaac atcaggtttc cgcgcttctc ggtgacggca cgctcgaagg tctcgtaatg
     7141 cacggggtcc ccctgggcga tgagcgcctc cagcgcccgt gcatcccggt agtatccccg
     7201 catgcccgag gcccgccgca ccacgtggct cgagggttcc ttcatgatcc cctggaccag
     7261 atcgaagtcc acgccaaagg gaaggtccgg tccccgtctc gattccgttg cgctcacgtc
     7321 ttctcccttc ctgctccgcg tccggcttcc gaccagcggt tcatcatccg caggagctcg
     7381 gcatagcgat cgatcctgcg ccgggcgacg gggacgcgcc gcgcgtccgg ctcggtgacg
     7441 gtccccgtcg cgacccagcg tgccgcggcc gccggcaggt ccgccaccgc gccgacgccg
     7501 tatgccgcaa gaagcgcggc accgcgcggc gcccctccgg cgcccgccac ctgcacgacc
     7561 ggcaccccga gggtgtcggc cttgatccgg ttccagacga cgctcttctc tccgccgccc
     7621 gtgacgcgga cctgtcgaac ggtgccggcg ccgaacagcg attccagcct gcgaaggtag
     7681 aggccgtact ccagggccac cccttccagg acggcgcggt agaggtgacc gggcccatgg
     7741 ccccacgcca gccccaccca ggcgcctcgg agatccggct ggctggggct caccctcccg
     7801 ccaagatgcg ggatgaacat cgggtcatcc gcgtcgggct ccacggccgc ggccaggtcg
     7861 ttcagtcgat cgagtccgcc ctcgacgtcc cccgcgaacc gctcgcggaa ccattcgagg
     7921 ttcatgccgc cgccggcgac ataggcgtac ggatgccaca gacccggcgt ggcggatcgg
     7981 ccgcagcaga ggatctgctc ttcgacgtcc ggccggaagg ccgacgtggt ggccgcgaag
     8041 accgaggcgg tgcccgccac atcggcgcag acaccggcct gagcggcgcc gcaggacagg
     8101 aagctggcgg ccgtgtcccc acagccggcg acgatgggca ccccgggcgg cagcccgcag
     8161 gccgccgcca ttccgcgcga caggccgccg atcacctcgt gggacaagac gatcctcgga
     8221 aggaggcccg ggtcgacgcc gaacaccctg cagagctcgg ggtcccaacg gggaccggat
     8281 gcgtcggcga aaccggagaa gtggaggtag ctcgcgtcga tgaaggcgtg agacgcgtcc
     8341 atcccgcaga gcctcatcgc cgcgtatccc ccgggctgca cgaaggctcg gatccgggag
     8401 aagctctcgg gtctctcgtg cttccaccaa agcaccttgg gtccgtggtt gaagctggcg
     8461 gggccgcccg tcttgcggac gatctccgcg ccggcttccc gcctcatatg cgtgatccag
     8521 ggcgcgcatc gagtgtccag ccaggaatcg tacggcgtga cacaccgacc atccgccccc
     8581 acgccgatga tcccggccat ctggccgtct atggcgatcc cgtcaacggc gatcccgggt
     8641 gcgcccccaa cggccgcggc gcgccccacg cattcggcca ggcactcgca gaccgaggcg
     8701 agctgtttct cgggatcctc ctcgacaacg cccgcggcgg gttggtgcag ctcggacttg
     8761 cgaaacgcct ccgcccggca acggccctcc gcgtcgtaga ggcccgcctt caccccttgg
     8821 gtcccgatgt ccactccgag gtacgtcatg tccgtgggaa ggagcgcgcc cggcgtcccc
     8881 gggccgatcg cgcctgcctt cggacggtac tttctgacgt ttctaccgaa tgttcaagcc
     8941 gatcggccca cgccctccag ccccttgggc gccagatcgc caatggctga ccgcaggggg
     9001 tccttcggat ccggcgcctt ggcgcgcggc ggaggcaccg gagtgtcgaa ctcgaaggtg
     9061 ggatctctct tctgccgctg gtaggtggcg cgcatctcca cccaggcgcc aatgagcgtc
     9121 cgcggccgcg gcatgtcggc cgcgatggcg cgggacatcc gcttgaggtt gtagcaggga
     9181 atggccgcgt acatgtggtg ctcggtgtgc cagttcatgt gccagtagag gaactcggtg
     9241 aagggatcca gcgtgatcga acgcacgcac ttgcgaaaat cggtgacgtt gctgcgcagg
     9301 cccgtgtgca tgggcatgag caccaggtac atccagagat tggccacgta ctggccgatg
     9361 gtgaccagca ccggcagcag ccagagccgc aggacgatgg aggcggcgat gaccagcgcg
     9421 tggaagagga gctggagccg ggcccaacgc acggcgagac ggcgcgccgt cgcgtcgtcg
     9481 gcgaacacgg cgccggacca ctcgttatgg aagcgcccgg tgaaggccag cgtcaccgtc
     9541 cgcgacagca cgatccagaa cgccgtgacg tcgaaggtca acagctccag gatccggagc
     9601 ggatgcaggg aaggcgtgat gggaagcacc acctcgcgat ctccctccgg gtgaagcgtg
     9661 tagacgtgat gataggtgtg gctgcgcttg tactcgtgcg ggttgtgcca ttggatcatg
     9721 cagtagatcc ggtggaagag cccgttcagc catttggtcc gaaagaccgt gccgtgcagg
     9781 agctcgtgag gcccgggcgt aaaggagaac accgtgccgt aggcgaagag ggccagggcc
     9841 atccagcccc acagacgctg ccggtagagg tgccaggtca ccaggccaag ggcgacgacc
     9901 agcgccagga agccgatcgt ctggagcgcg ccttgggcgt cgctgcgccg ggtgagctcg
     9961 cgaagccggt ccggcgggat ggggcatcga tacccggcca ccttcaggga ttcccgcaca
    10021 tgcgccatgg gggtccgctg ttctgacatc ctcgtctcct ccggcgccaa tgtactgtgg
    10081 tcggatcggc ggaggaaacg gctcagccgc gggaccgggt gcccggatca cccccgcgcg
    10141 acctcacggg actcggcgaa cagccttcgg gtccgcggat cgatatcggt agacggcccc
    10201 tgaatccgca aggatccctt cagacggatg tcctcggacg aagagcccac caggacgtca
    10261 acgcttcccg gatagagccg aagcttcatg tccagatcgg gaaaggcgag ctcgtcggtc
    10321 gccagctcga tctcgacgtt gacttcctgt ccaggctcca gcacgaccct atggaagccc
    10381 ttgagctgcc gcaccggccg ggtcacgggg gtgccctcgg gccgataccc gacgtaaagc
    10441 tgagctatct ccgctgccgc ccgggtgccc atgttgcgga tcgagaagga gacccttgcc
    10501 gttccgcggc ccgtcacctc ccccggagaa acgcaaaggt ccttatacgc gaaggtggcg
    10561 taggaaaggc cgtgcccgaa cggatagagc ggtgaggcgg gacgatcgac atagtctccc
    10621 gtccagtgcg accgcccgcc cgacggggtg tgatagttgt acaccggcac ctgtcccgcg
    10681 gaacgcggga aggagatggg aagcttgccg ctggggctga atcgaccgac cagcgcatcg
    10741 gccaccgcgc ctccgcctgc ctgacccggc agccaggcgc agaggagcgc cgcgacccgg
    10801 tcgctgaacg catccagcgc gtacggccgc ccgacggcaa gcaccacgat caccggggta
    10861 cccgtttgca ggatcgcctc aaggagcctc tcctgcacac cggggagcct gaggtccgca
    10921 cagtcccgcg attcgccaga ggtgcattcc gtggtgaggc cggcacggtc cccgagaacc
    10981 agaatggcca cctgagactc ccgggccaag cggaccgctt cggggatgcc atcttcccgc
    11041 tcggtcttca cgtcgcagcc gcgggcatgc gtgatccgaa cgccgccgtc cgatacctcc
    11101 cgcagcgctt ccaggaccgt cgggatgcgc agagaccggc tcacgtcccc cagatcatcg
    11161 gggatcggca tcccgaaggg gttgtcctcc ttggtggcgg caaggacctc cacgtgacag
    11221 gggtacgcgt agtcgccgat caggtttcgc cagtcgtccg cggacggccc gatcagggcg
    11281 atggatcgcg tgtccgggga gaggggcagg atgccgttgt tggacagaag gacgatggat
    11341 tgccgggcaa gccgggcggc gcactcgttc tgggcggggg tgtcgaagag ctccaggaca
    11401 cgaccctccg gaacaaacgg ccgttcgaaa atgccgaggc ggaacttggc ggtcaggacg
    11461 cgaagcgcgg cacggtccac gtccgccatg ccaacgaggc cggcatcgag agcctgggtc
    11521 aacgactccc cgtagctgct cgtggagggc agatcgacgt cgatgcccgc gcgaagcgcg
    11581 aagcctgccg cctccgcgcc agaccgcgct acgtggtgat accgctccag catggggatg
    11641 gcccagtagt ccgacaccac gatcccatcg aagccccact catccctcag aagctgggac
    11701 atcaaccaac gggaggcacc gcagggcacc ccgtcgatct cgccatagga gttcatcacg
    11761 gatcccaatc gggccatccg aaccgcggcc tcgaaggggg ccaggtagac ttcggccagc
    11821 tcccgcggcg gtatcttggc gggagcccag ttcattccgc cctcggcccc gccgtacccc
    11881 acgaagtgtt tggcggtcgc gacgaccccg tccggcgcat cgcctccctg gatgccccgg
    11941 acatacgcgc accccagcag ggatacgagg taagggtcct caccaaaggt ttcctcggtc
    12001 ctcccccacc ttgggtcccg gcacacgtcc agcaggggcg cgagcccctg gtgcgcgccg
    12061 atggcgcgca tctggccgcg gatggcggcc gccatccgct ccaccagctc ggggttccac
    12121 ccggccgcca cgccgatggc ttgcgggaag agcgtcgcgc cccagctcat gagccccgag
    12181 cagatctcct catggacgat ggctgggacg ccgagccgcg tatgctcccg gagatgcctc
    12241 tgaatctggt tggccagggc ggcgcaacgg tccggcttca gattggtggc tccgccgagg
    12301 cgggtcacct gccctatccc gttcgccagc ctcgcctccg ccttctcaga cgagaaggtg
    12361 aacccggaga gaagctcgaa cgaccagcag ctcccgagct gcgccgtctt ctccgccgga
    12421 gtcattcggg ccagcaggtc ggccgcgcgc gcctttggcg aacgggacgc gtctcggtac
    12481 agagggtcgc tcatgcccgt gcccccgtga ctcctccgga atacagatgg catctgacca
    12541 gccggccgtc gtgctcggca aggggcggct ccacggcccg gcagacctcc atgacgtggg
    12601 gacagcggcc ggcgaacttg caggcagcca gtcgatactc cgcctgctcg ccgtccgcca
    12661 tcgcgatgtc ctggttccac ttcagcgccg gatccggttc cgggacggat tccttcagga
    12721 tccgtgtgta cggatgctcc ggatgatcga ggaccttctc cacgtcgccg atttccacga
    12781 tggtgccccg cagcatgatg gcgatccggt cgctcacgta gtacgccgtg gcaaggtcgt
    12841 gggtgatata gaccacgctc acccggtgct ccgcgcggag ctccttgaac aggttgacga
    12901 tggacatccg cagggaggcg tccaccatcg acacgggctc atcggcgatc agcaccgaag
    12961 ggttggtgat caaggagcgc gccaccgaaa tcctctggag ctggccgccg gagaactcgt
    13021 tggggtactt ccccagcacc gcgtccaggt tcaaccccac gcgctcgagc gccgcctgga
    13081 cgggccgacg ggcggccgat cgggagggag ccatgccgaa gttcacggcg gtctcgaaga
    13141 ggtagttctc caccttcttc aggggattga aggtctcgaa ggggttctgg aataccggct
    13201 ggatctcccg catgaactgc atccgctggg cgcggccccg gatccgggtg atgtccgctc
    13261 ccttgtacga gatgcttccc tggctcggct ccacgaaccc caggatcatc ttggcgatgg
    13321 tggtcttccc gcttccgctc tccccggcca gggtaaagac ctggggggaa tcggctggca
    13381 ggacgaagct cgcgtcgttg accgcgacga tcttctgccc cgcgaagatg cggccgagcg
    13441 tgtagacccg cgtgaggtgg tcggcccgca ggagggcgtt atcgctcata gcgcgtgtcc
    13501 tcatcgacaa agcaggccac ccgatgccgg ggaccgatgc ggacgagccg cgggttgcgc
    13561 acgtggcaga gctccttggc ctcggggcag cgtggatgga accggcaccc ggacggcgga
    13621 tcggtaaggg acggcggggc cccgggcgcg ctcacgcgcg gcgtcttgtc tccgatgctc
    13681 ggcagcgatc gcaggaggaa ccgggtgtag gggtgccgtg ctcccttgaa caggtcggag
    13741 acgggagcct cttcgacgat ctcccccgcg tacatgatgg ccacccggtc gcagatgttg
    13801 gcatggattg ccatgtcgtg cgtcacgagg atcacggtgt tctgcatctc ctcccggatg
    13861 cgcttgagga gctggatcac gccgcgctgc atgatgacgt ccagggccgt ggacggctca
    13921 tcggccagga tcagccgcgg ccgcaggatg gtggccagcg caatcgtcac ccgttgacgc
    13981 atgccgccgg agagctgatg ggggaaggcc ttcaggaccg ccggagaaag cccgagggac
    14041 cgcagatgcg cctccacgag gtcctggaac tcgccgcgtc cggcaaagcg gcgatgcgcg
    14101 ctcatgaagt cccagaagga cttgcggatc ttgcggaccg ggttgaggac gctcatggat
    14161 ccctggggaa tgtacgacac cagcctgccc cgcacctgcc gggcctcgga ttcccgccgc
    14221 aggctcagga tgtcgatctc tcggttctcc agcgtatagc ggaccgagcc gcccatgatg
    14281 gtgagcgggg gctggaccag gccggtgagc gtcttgatga gcgtggtctt cccgcagccg
    14341 gactctcccg cgatcccgta gatctcgttc tcctcgacgt ccagcgttat cccgtccacg
    14401 gccttcacgt ggctctgcag gccatacagg cgcgtgaggt agtgggcgcg caggttctcg
    14461 accttcagga ccggcatcgc ctactcctcc ctcaggcgga tccgcatgag cctggtccgt
    14521 ggatcgaggt attcactgat gctcacggag agcaggtaca gcgcgaggat caggagcacg
    14581 cagaggacca ccggagtgag gatccagttc cagatcccca ggaagatggc ctggtactgc
    14641 attgcccagt agatcatggt ccccagggtc gggatctcga ggctggagag gcccaggacc
    14701 gccagcgtca cctccatgcc gatggcccag aggatgttgt tcaggctcgc ggccatgacc
    14761 aggggaacca cgaacgggag gtgctcggag aacacgatcc gcacggcttt catgccggaa
    14821 agcaccgcgg tgttcgtgaa ctcgcgttcc cgaagggtca gcaccatgga acggaagagg
    14881 cgggcatccc actgccagcc gaacaggccc agcacgaagc ccaggatcgt gacgttcatg
    14941 tggcccttga ggatggcgga gaccaggatc aggagcggga agatcgggat gatcacgaag
    15001 ctgtcgttca cggtcatcag gatccggtcc aggatcccgc cgcggtagcc ggccaccagc
    15061 ccgatcacga gcgcgatcac ccgggagatg gccgccgcca ccagcccgag gatcagggag
    15121 ttgcggatgg ccaccgtggt ctgccagaag atgtcctggc ccatggagtt ggtgcccagc
    15181 gggttcgtcc acgatatcgg ccggtcccgc ggaaacacgt tccacagctt ggggtcccag
    15241 gggatgaaga gcgaaaggat ggccagggcc accagggcgg cggtgaccac gaacccgaag
    15301 cggaacctgc cgtcgtggcg caggagattg ccaacggtac gcagcgcgcg catgggtcct
    15361 cctagcggtg ccgcacccgc gggtccagga gcgggtagat caggtcgatg acgaggaccg
    15421 ccaccgcgat ggaaacaatg gaatacacgg tgattcccat gatcacgttg tagtcgctgc
    15481 tgatgacggc gttgtacagg agcgttccga tccccgggta ggcgaacacc tgctcggtga
    15541 tcagcgcgcc gccgaagatg aaccccaggc tgagggccag gccggtgacc tgggggagga
    15601 ttgcgttacg ggtgatgtac tgagtgacga tcttgccgcg gggcaccccg gcggcctcgg
    15661 cgtaggtgac atagtcttcc gccacgacgg tgaacgtcac cgatcgcatc tggaggaacc
    15721 acgtgccgaa cccggtgagc acgagggaaa tggcgggcag gaacgcgtgg cggaccacgt
    15781 ccaggacgta cttgagcgtg accgcgcctt tgagcttcac cgagtagccg ccgatcatgg
    15841 ggaacaccgg aaagacgtac gcgaacagga tcaccagggc cagcgccatg atgtagtacg
    15901 ggacgggccg aatggtcatg gagaagatct cgatggcgcg ggcccacttt gcccgacgaa
    15961 aggagccggc cagtccccca aggaggctgc cgatggccca ggagagaaac gtggtgatga
    16021 gcatcagccc gatggtccag ggcatggagt tgcggatgag cgtggtgacg ggcgtgggaa
    16081 agtgagagaa ggacgggccg aagtctccgt ggaagagccg ttcccagaac agaccgtact
    16141 gctggaggaa cgtgccctcc aggccgtaca actcccgcaa ggtctgcttg atcttgccga
    16201 tcgtggccga gtccaggtag gcgccccgcg tggtcaggtt ggcgagctgc cgctcaatgg
    16261 gatcgttggg agtcagacgg gggatgatga acaccaccgt ggcgccgatg aaaatgacca
    16321 cgacgaactg cacgagccgc ggcaggagat atcgcttgag aaactgcatg cgaaggtccc
    16381 ttcaggaagg aatgcccctc ccgcacggga ggggctgatt cgtcctctgg gtcttactgc
    16441 ttgcccgtcg gcttcagtcg ggggagcatg aacttgaagt tgggccagtg atagtggggc
    16501 tgcgtgtact gattgccccc gcccggatag ttggtccagt agtactcatc ccagccgagg
    16561 acggacacgt aggatcccag gctgatgccc ggctgatact tcaccaggac cttcaacccg
    16621 gcgaggctga gctgctggct cttgggatcc agggagggca cctgctccat ctggtcgatg
    16681 atcttgtcca gctccggcgt ggagaaacgc gcggtctctc cgccggccag ggccgttccc
    16741 aatggaacca ggtacttgga gtggtactgc tgcatcacgc tgaacaggtc ccagtcaccg
    16801 ccgttggccc acatcggcca ggcgccggaa acgtcgaagg acccgttgga gtccaccgtg
    16861 gaggactgct cgttggcgtc cacgttcacg tcgatgccga acttcctcca ctggtcgccg
    16921 agctggaagc cccacttgaa cgacggatgc gccgggttgg tgggggcgtt gatgtggatg
    16981 ctccagggct tgccgtccgg tagaagccac ttgccgttct tgtcccggga gaatcccttg
    17041 cccttcagga gcgtctcggc cgccgccgga tcgtacttcc atccgccgta gccgaacatc
    17101 tcgttgatcc ccgcctgggt ggtgggcacc gggtagccgg cggccttggc ctgcctggcc
    17161 agggtgttgg gcatgtcggg atcgtacggc ttgtagggct tgccacccac cgtaatcgtg
    17221 aagtccttca gccactgctg catgggctgg tagtaccact tgtagaacgg cagcgtagcc
    17281 ggaacgtacg ccggtgccag cgccgtggcg ccgttgtacg cggtcatgac catctggggg
    17341 atgttgaggg ccagattcag ggcccagcgc acgtccgcgt cgttgtacgg ggcaacctgg
    17401 cagttgaaca ctccgccggt ggtgcagggg tgcaggatct cgtaccaggg gaagtccttg
    17461 tacagcccgc ggaccgtggg gaccttcttc tgggcggcga caaaggcctc cggtgtcagg
    17521 tcgcacatgt cgagctgatg ctgggattcc gcgatgacct tcttgtccgg gtcgccgtag
    17581 tagacgaact gcacgtaggt gggcgccggc ttgccgtaga gcatcccggc ggggctcttg
    17641 tcccagtccg cgcgccgctc gtaggtgaac cagtagccgt cggtatcgta gtccttgagg
    17701 acgtacgggc cggtgccgac cgggggatag aactggaagg tcatagggtc tgcctgcttc
    17761 tcccagatgt gcttcgggag gatacgcagg gctccccagc gatcgttgaa gaacacgtcg
    17821 aacttggcgt tgggcttctt gaggtcgaac acgacggtgt agtcatccgt cttggagacc
    17881 ttggccacgt tgtccgtcat ctgggcgtag taggtcattc ccttgttggc catctggacc
    17941 tggaccgtgt agaccacgtc gtccgcggtg aaggggacgc cgtcgctcca cgtcacgcct
    18001 ttgcggagct tcgccgtcat ccgggtgaag tccttgttgt agatcggcgg atccgaggcc
    18061 agcgtaggca cgttctggcc cagagtgtac tcgaccgtcc acagcgggtc cgcgacgagc
    18121 tgttgcatgc ccttgtcgtt gccgacccac ccggc
//
LOCUS       DASRZS010000202         3070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2803245, whole genome shotgun sequence.
ACCESSION   DASRZS010000202 DASRZS010000000
VERSION     DASRZS010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2803245"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..2297
                     /gene="metG"
                     /locus_tag="VFH83_05075"
     CDS             72..2297
                     /gene="metG"
                     /locus_tag="VFH83_05075"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET6485766.1"
                     /translation="MERRLITSALPYVNNIPHLGNLIQVLSADVFARYCRLAGYETLY
                     ICGTDEYGTATETRALEEGITPRELCDRYHAVHRDIYQWFQIAFDHFGRTSVPVHTEI
                     TQHIFQRLHENGYITERVMHQPYCEKDRRFLADRYVLGTCPHCGYADARGDQCENCGK
                     LLDPTELIEPRCSVCSSRPVIRETKHLFIDLKAILPKLRNWIESAAEKGFWARNAIQM
                     TYAWMRDGLKERAITRDLSWGIPVPLDGYRNKVFYVWFDAPIGYISITAELTPEHWKS
                     WWYAPRSVKLFQFIGKDNIPFHTVIFPSSQLGTGEDWTTLYHMSSTEYLNYEGGKFSK
                     SKGVGVFGTDAKETGIPADVWRFYIYYNRPERSDALFTWKDFQEKVNSELIGNLANLV
                     NRTLQFVSRFYQGALPASEPDPDFWTEVRAAETEISGLLEKVELRDAFRRIFALSSFG
                     NKRFQDGEPWKGVKENPQATARLIWNLTYLVRDLSILIRPYLPITSDRIGKTLGLAPT
                     TWKDLGDPKGIERVEKPELLFARLEDKEVEALRQRFSGTQKERAQAAEGAAAALGGTS
                     PTAAPAPTPPSLSPAEISAGFRGQVDLRVARIIEVKRHPKAEKLYIETVDLGTEQRTI
                     VSGLVPFYKEEELLGHNVVLVANLKPAVLRGVESNGMLLAASAGDTVEVLFLDDARPG
                     DRVAPSGAASEPAPAQIDIDRFLSLPITVEDSRVVTGDAALECGGRPLVTARVSKGRV
                     K"
     gene            2243..>3070
                     /locus_tag="VFH83_05080"
     CDS             2243..>3070
                     /locus_tag="VFH83_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HET6485767.1"
                     /translation="MRRQAARHGSGVEGQGEVIEASPAPLSALDRSAELLQTGFWGSF
                     KQAHGWKASAFSVRPEAGTPFDLLVLSRRIARAASLAYIPFGPRADPGDARGTFLSEL
                     AAAIRPELPADTLLVRFDLPWAKEGGAPSEGSRRPMVRHASMDVQPPNTVIVDLSPEP
                     DAILAAMKPKTRYNIRLAEKKGVKVREALPGELDAWYELYRETGRRDRIAIHSASYYR
                     ELLQAPYPGDRPSIVLLVATHEGDLLAGNIVSFWKESATYLYGASSGKKRNLMPTYAL
                     "
BASE COUNT          555 a         1013 c          978 g          524 t
ORIGIN      
        1 cggttcgaat cgcgacttgc gggccggttt cttgacgggc attggcgcct cggctacagt
       61 tgcatccact tatggaacga cggttgatca catccgccct gccttacgtg aacaacatcc
      121 cgcacctggg gaacctcatc caggtgctgt ccgcggatgt gttcgcccgc tactgtcggc
      181 tggccggcta cgagacgctg tacatctgcg gaacggacga gtatggaacg gccaccgaga
      241 cccgcgcgct ggaggagggc atcaccccga gggagctgtg tgaccgctac cacgccgtcc
      301 atcgggacat ctaccagtgg ttccagatcg cgttcgacca cttcggcagg acctcggtgc
      361 ccgtgcacac ggagatcacc cagcacatct ttcagaggct ccacgagaac ggctacatca
      421 ccgagcgcgt gatgcaccag ccctactgcg agaaggaccg acggttcctg gcggaccggt
      481 acgtcctggg gacctgcccg cactgcggct atgcggacgc ccgcggcgac cagtgcgaga
      541 actgcggcaa gctcctggat cccacggagc tgatcgaacc gcgctgcagc gtgtgctcct
      601 cccgacccgt gatccgggaa accaaacacc tgttcatcga cctcaaggcc atccttccca
      661 agctccgcaa ctggatcgag tccgcggcgg agaaaggctt ctgggcccgc aacgccatcc
      721 agatgaccta tgcctggatg cgggacggtc tgaaggagcg cgccatcacg cgggatctga
      781 gctgggggat tccggtcccg ctggatgggt atcgcaacaa ggtgttctac gtctggttcg
      841 acgcgcccat cgggtacatc tccatcacgg cggagcttac gccggagcac tggaagagct
      901 ggtggtacgc ccccaggtcc gtcaagcttt tccagttcat cgggaaggac aacatcccgt
      961 tccacacggt gatcttcccc agcagccagc tcggcaccgg tgaggactgg accacgctct
     1021 accacatgtc ctccacggag tacctgaact acgagggcgg gaagttctcc aagagcaagg
     1081 gcgtgggagt gttcgggacc gacgccaagg aaacgggcat ccccgcggac gtatggcggt
     1141 tctacatcta ctacaaccgg ccggagagat cggatgccct gttcacgtgg aaggacttcc
     1201 aggagaaggt caactcggag ctgatcggca accttgcgaa cctggtcaac cggaccttgc
     1261 agttcgtgag ccgtttctac cagggagccc tgcctgcctc ggagccggat cccgacttct
     1321 ggaccgaggt ccgcgccgcg gagacggaga tcagcggcct tctggagaaa gtggagctgc
     1381 gggacgcgtt ccgccggatt ttcgccctct cctctttcgg caacaagcgg ttccaggacg
     1441 gcgagccctg gaagggagtg aaggagaatc cccaggctac ggcccgcctc atctggaact
     1501 tgacctacct cgtccgggac ctgtccatcc tcatccgccc gtatctcccc atcacttcgg
     1561 atcgcatcgg caagacgctg ggtctggccc cgaccacctg gaaggacctc ggggatccga
     1621 agggaatcga acgggtggag aagccggagc ttctcttcgc ccggctggag gacaaggagg
     1681 tggaggctct acggcagcgc ttctccggca cgcagaagga gcgcgcccag gccgccgaag
     1741 gggccgccgc cgctttggga gggacgtcgc ccacggcagc cccggctccg actcctccgt
     1801 ccctatcacc ggcggagatc tccgccggct tccggggcca ggtcgatctc cgcgtggccc
     1861 ggatcatcga ggtcaagcgc catcccaaag cggagaagct gtacatcgag accgttgacc
     1921 tgggcacgga gcagcggacc atcgtctcgg gtctcgttcc cttttacaag gaagaggagc
     1981 tcctgggaca caacgtggtg ctggtggcca acctcaaacc agcggtgttg cggggcgtgg
     2041 agagcaacgg gatgctcctg gcagcctctg ccggggatac ggtggaggta ctgttcctcg
     2101 atgatgccag gccgggagac cgcgtcgccc cgagcggagc cgcatcggag cctgcccccg
     2161 cgcagatcga tatcgatcgg ttcctgtccc tgcccatcac ggtggaggat tcccgagtcg
     2221 tgacgggaga cgccgccctg gagtgcggcg gcaggccgct cgtcacggct cgggtgtcga
     2281 agggcagggt gaagtaatcg aggcgtcccc cgcgccgctt tcggccctgg accgaagcgc
     2341 cgagctgctg cagaccggct tctgggggag cttcaagcag gcgcacggct ggaaggcttc
     2401 cgctttctcg gtacgaccgg aggcggggac cccgttcgac ctcctggtcc tttcccgacg
     2461 gatcgcgcgg gctgcttcgc tggcctatat acccttcggg ccgcgcgccg accccggtga
     2521 tgccaggggg accttcctgt cggagttggc cgcggcgatc cgacctgagc tgcccgcgga
     2581 cacgctcctg gttcggttcg atctgccctg ggcgaaagag ggcggcgcgc cttcggaggg
     2641 ctcccgcaga ccgatggtgc gccacgcctc catggacgtg cagccgccca acaccgtgat
     2701 cgtcgatctc tctccagaac cggatgccat cctggcggcc atgaagccaa agacccggta
     2761 caacatccga ctggccgaga agaagggcgt gaaggttcgc gaggcgctgc ccggcgagct
     2821 cgacgcatgg tacgagcttt accgcgaaac ggggcggcga gaccggatcg ccatccactc
     2881 cgcctcctac tatcgcgagc tgctgcaggc cccgtatccc ggcgatcgcc cttccatcgt
     2941 gcttctcgtg gcaacccatg agggcgatct gttggcgggc aacatcgttt ccttctggaa
     3001 ggaaagcgcc acctacctgt acggcgcctc atcgggcaag aagcgcaacc tcatgcccac
     3061 ctacgcgctg
//
LOCUS       DASRZS010000203        11582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1647229, whole genome shotgun sequence.
ACCESSION   DASRZS010000203 DASRZS010000000
VERSION     DASRZS010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11582
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647229"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFH83_05085"
     CDS             <1..249
                     /locus_tag="VFH83_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET6485768.1"
                     /translation="FRAHNLLGDQPPAGPFDVIFCRNVMIYFDKPTQRRLVDEYFARV
                     LDPKGYLFVGHSESLSGTSEKFRYLRELKAPIYQRKEE"
     gene            257..1348
                     /locus_tag="VFH83_05090"
     CDS             257..1348
                     /locus_tag="VFH83_05090"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HET6485769.1"
                     /translation="MIRVMVVDDSPLVRKIASDILNSDTDVTVACTAPTAEIALAKLD
                     RYQPDAITMDMEMPGMGGVEAIRRIMRQRPTPIIVLSAHARQGADLTLRALEAGAVDF
                     MLKPSSNLSGGLDAVAAELIEKVKSASTVEVKAPEREEGWDAQPKTVERKPAPRIVRS
                     CDDRFDLVAIGTSTGGPVALKTVLSGLPAEFPLPIVIVQHMPPVFTKAFAERLNSTCA
                     VAVKEAEPGDVLRPGLAIVAAGNYHLTVSRSHGEARVQLNQDPLVSGHRPSVDVLMRS
                     VAQEYGARAIGVIMTGMGKDGSDGLRELRKKGGHIVAQDKASSVIFGMNREVILNGDA
                     NEVVDLTEIPRVLAELSCAKAYAKEAAAL"
     gene            1368..1898
                     /locus_tag="VFH83_05095"
     CDS             1368..1898
                     /locus_tag="VFH83_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET6485770.1"
                     /translation="MERMNRRDEVEGENSQLISFAVGDEEYGLEILLVKEVIRMREIT
                     RLPKQPTFIKGIINLRGDVIPIVDLREKFGLERHVYSATTRVIVVDVEEKLVGMVVDA
                     ARQVVRVPADQIDPPPAIVGGMSTDYIKGVGKLDERMIILLNIDRILSREEKLQLQKM
                     AGASEGAAEMEPALQA"
     gene            1975..5169
                     /locus_tag="VFH83_05100"
     CDS             1975..5169
                     /locus_tag="VFH83_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6485771.1"
                     /translation="MSTVIASGESDRGATLTPAAVPSAPAAASQASAATSPAKGPATE
                     TELGEEARLTVHRVLEAFDASKTGLESLHKDLFHPSPRVVTSALSALSKLRDQRSMRF
                     VARLLTSANEQIRCAAVRTTAEIRHPQAARILFDLLKITTGDATRRAALEALAVVAPE
                     TPQLRASLQEYSRSSTVSGSTRALATGLLLRTGGLKEPGDLLDSAEEEVIESLFTQAQ
                     TSPEIADHVVRDGISHYHRLSAKHRALLLSLAAPFTVPGSADLLLWAVSDADQDVRRA
                     AYQLLAQPSTKPSNLETILKTIADSVDPTPALEDEAIGALQRMEPREEQSAMGEKLLG
                     RIQELFQTVSREDRRVVSDSHELGWLISRSKEYVEYYLDEDVRHALVQYLKGVGGYSK
                     ARLLQSLRETAVRVEVRHFDGYRALADLIEEPKRHGIALVVRELAIARLGRRKPLSLL
                     IRNLWISRLLRTTGSPEAQEQLFLEIYTWSSQAKIFRLSEAALFALSRVNLARTAQLC
                     RECMRTPVVSKLLAIASVHLLKDLDWSSMESSVLGLLTGADDTNVLLNLFEALASSDR
                     SLNGEILKTLLGVVRLGPNRQVVQEAAELLAGQPGSEALDAVKEAFDRAEPWRQAILL
                     SIVERRVREGNVSNREGLIEFLYKVLRQQFSSLDGRAAILLWRLGDDYAQQLLRDLSR
                     RGGLEQRTELVRGLKGFVTEEMAELLAPLARVESSSLQEVLRDTLLGAEDAGAQRRIC
                     ELVLTTQDPSEEDDEADAGDVQVDLAKEKRAYQFEKTFIQELAVLFTDIVGYSKKAQS
                     LTSMQLTSMIQDYEGVLLPTVSTHQGELIKRMGDGHLFVFRSPLNAVLAAIRVQKALK
                     RFNSYREESTRLAIRAGIHWGKVVRKEGDVLGNNVNIASRLESNARPGSVLISDAVQA
                     MLGGHIHCREIGFIRVKNIAEPIKVHEPYELVLDFPEELDPLKALKKDGGEHSAEEAA
                     GAAEPSEQPGEGTRPPQSAAPAHGDPKPAEGNGQRATRSLDGETARYLTETFTCLNAI
                     CRAAEANQRPMSDVRKELATRWAGLREMLQRPGA"
     gene            5282..6040
                     /locus_tag="VFH83_05105"
     CDS             5282..6040
                     /locus_tag="VFH83_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HET6485772.1"
                     /translation="MQNVLLAWFSVNGTTTAIAERIAAGLAKAGCAVTPIRIGKEKPP
                     DLAAFDTVGIGTPTYVFRPPFVVTDFLRSLPDLTGKQSFAFVLHGTDRGACANQVRKT
                     LARKHARDLGLYHSFGSDLFNGYLRRGVLFSPDTPRAQDLDAAERFGTSLAERGPGGA
                     RVPEPMDGPTHLIYAIERLSASRALTGLLWSRAFHADERCDNCGVCIAACPMHNITAQ
                     KDARPRWETHCLLGERDGIPFVRVTHSGGVTRRL"
     gene            6193..6822
                     /locus_tag="VFH83_05110"
     CDS             6193..6822
                     /locus_tag="VFH83_05110"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET6485773.1"
                     /translation="MESHTVTGGKDPIRLFRSDFLEFFTHVHPAVVLAVWLPVAAAFL
                     TWSALQSAPRVWLSVPVCWVAGLASWSLVEYVLHRFIFHFRPRTPRQERLSFLVHGVH
                     HAQPLVKTRLVMPPVVSVPLALFFFGLSWLIGSRLLGKPVWADSYFAGLVVGYVAYDM
                     LHYSMHHFQLRSPLLSALRKHHMQHHAVTWDKRFGVSSPVWDHVFGTEP"
     gene            6921..8264
                     /locus_tag="VFH83_05115"
     CDS             6921..8264
                     /locus_tag="VFH83_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6485774.1"
                     /translation="MADPDWDSLTQETVRNLSRLLQAQTANPPGNEEPAVEVIRQILE
                     ANGFPSRDLAIVESAPHRVNLVARLPGDGSLRPLLLSGHTDVVPVERERWARDPFGGE
                     VVDGEIWGRGALDMKGSLAMYLEVFLMALRSKARFRRDLILAAVADEEAGFTHGSKFL
                     TDHHRELIEAEYGITEAGAITVYVGKARLYPIQVAERGICWLRMTASGKPGHGSMPHP
                     DNAILHLAEAIEKIRRVRHLPVHLTSTFLKMMEAASRQVAFPLSLVPSLLRSPGLAGF
                     LLNRLPEESRGLLSAMVANTVTPTILKAGVDTNVIPSVAEALLDCHKLPGQTSQDVMR
                     EIGAITGNSVTLEVVRESPGTEFPVDSPFYRTLEAAARRMDPQGIFVPMLMPAATDAS
                     HYQRAGVSVYGFTPGVLPPEFPTFQIGHGHNERLPVSSIRSGLPALWEVLTNTCA"
     gene            8281..9294
                     /locus_tag="VFH83_05120"
     CDS             8281..9294
                     /locus_tag="VFH83_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485775.1"
                     /translation="MTSELLILICQTAVVYLLVLGMHSLRRRTGLSLFYALLGGLTAI
                     MSWVTDAGLSVHVGGVTFVVGSTVFYTALLLGVFVVYVFDGPLSARTAILGVAVVSTL
                     TPLVSAFLHLQAWAAGSRLLPFIPTPSLRINAASVAATLVDMVFLAVAWEYLGRPRIH
                     RWLRVYLTLLGVLCLDVVLFNTGAFAGTGSFLTIVQGTLISRVSMSVVALPFLLVYLE
                     WQSRWKGEAIENRPVLAILRQVAKIEAELSRAQVEIELRKAAERQRDETIRELRQALS
                     EVKTLRGLLPICSRCKRIRDDSGYWNRIETYIRDHTDARFTHGICPDCARELYPDVPI
                     DPE"
     gene            complement(9302..10534)
                     /locus_tag="VFH83_05125"
     CDS             complement(9302..10534)
                     /locus_tag="VFH83_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6485776.1"
                     /translation="MKKAGTVRRTRDNATLLNTILEKQERLHHIRDLNTLLDSVLQET
                     RSLTDADAGSIFLVEGKKLLFSYVQNDSLFKKDFLSNKYIYTNNSIDIDEKSIAGYVA
                     STGESLMIEDAYQLDQDYPFSFNPYFDEISSYRTVSMLIVPIRTSTNNMVGVLELINR
                     LSSAGDPIPFSSDQRFYITQFAFYAGIAIERALLLRDVVFKMVRTTELRDPQETQPHV
                     SRVGSYAVELYQKWAERHGVPREEITNFKDILRISSMLHDIGKVGVSDAILKKREDLT
                     AEEYESIKYHTVFGAQFFENPTSEWDTLAYEVALNHHERWDGGGYPGVVKDIHAKGLK
                     FGPGKKGSAIPLSARIVTLADVYDALICKRAYKDAWEEDRVLFYIKEQSGRQFDPELV
                     KVFFEIYEVIRAIREKWA"
     gene            complement(10531..11436)
                     /locus_tag="VFH83_05130"
     CDS             complement(10531..11436)
                     /locus_tag="VFH83_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET6485777.1"
                     /translation="MADFEILDSKYGSLVPSNSFLEKLWTGARWTEGPVFFADLDCLL
                     FSDIPNNRMLRWSEGAPVTVFRSPSNNANGNTRDRQGRLVTCEHGTRRVTRTEPDGTV
                     TVLADAYRGKRLNSPNDVVVKSDGSIWFSDPPYGILTDYEGHKSAQEQDRCYVFRLDP
                     STGTLAAVADDFERPNGLAFSPDESILYVSDTSHADRPGGPPHIRAFSVKDGKTLTGG
                     RVFADMRQGRSDGFRLDTDGNIWTSAGAGVHCYTPAGDLVVRIPIPEAVSNVAFGGPK
                     RNRLFITGFTSLYSIFLGVNGVQRP"
BASE COUNT         1942 a         3767 c         3880 g         1993 t
ORIGIN      
        1 ttccgggcgc acaacctgct gggagaccag ccgcccgccg ggccgttcga cgtcatcttc
       61 tgccgcaacg tgatgatcta cttcgacaag ccgacccagc gcagactcgt cgatgagtat
      121 ttcgcgcggg tgctcgaccc caaggggtac ctgttcgtgg gtcattcgga gtcgctgagc
      181 ggaaccagcg agaagttccg gtatctgcgg gagctgaagg cgcccatcta ccagaggaaa
      241 gaggagtgag ccgaacatga tccgagtgat ggtggtggac gattcgccgc tggtgcgcaa
      301 gatcgccagt gacatcctga acagcgatac ggacgtgacg gtcgcctgca cggcgcccac
      361 cgcggagatc gccctggcca agctggaccg ataccagccg gacgcgatca cgatggacat
      421 ggagatgcca ggaatgggcg gggtggaagc aatccgcagg atcatgcggc agcggcccac
      481 gcccatcatc gtgctgagcg cccacgcccg ccagggggcg gacctcaccc tgcgggcgct
      541 cgaggccggg gcggtggatt tcatgctcaa gccgtccagc aacctctccg ggggtctgga
      601 cgcggtggcc gcggagttga tcgagaaggt gaagagcgcc agcaccgttg aggtgaaggc
      661 gcccgaacgg gaggaaggct gggacgctca acccaagacc gtggagcgca agccggcccc
      721 tcggatcgtc cgttcctgcg acgaccgctt cgatctggtg gccatcggca cctccaccgg
      781 cgggccggtc gcgctgaaga cggtcctctc gggtctgccg gcggagtttc cgctgcccat
      841 cgtgatcgtg cagcacatgc ccccggtatt cacgaaggcc tttgccgagc ggctcaactc
      901 cacctgtgcg gtggcggtga aggaggcgga gcccggcgac gtgctgcgcc ccggcctggc
      961 catcgttgcc gccggcaact accacctcac ggtgagccga tcccacgggg aagcgcgggt
     1021 ccagctgaac caggacccgc tggtgtcggg tcatcgcccg tcggtggacg tgttgatgcg
     1081 ctccgtggcg caggagtacg gcgcccgagc catcggcgtc atcatgacgg ggatgggaaa
     1141 ggacgggtcg gacggtctgc gcgagctcag gaagaagggc ggccacatcg tggcccaaga
     1201 caaagccagc tcggtgatct tcggcatgaa tcgggaggtc atcctgaacg gagacgccaa
     1261 cgaggtggtg gacctgacgg aaatcccaag ggtccttgcg gagctttcct gcgccaaggc
     1321 gtacgccaaa gaggccgcgg cgttgtagag acgaggaggt cgacatcatg gagcgaatga
     1381 accggagaga cgaagtggag ggcgaaaaca gccagctcat cagcttcgcc gtcggcgacg
     1441 aggagtacgg gctggagatc ctgctggtga aagaggtgat ccgcatgcga gagatcacgc
     1501 ggctgcccaa gcagccgacg ttcatcaaag ggatcatcaa ccttcggggg gacgtcatcc
     1561 ccatcgtgga cctgagggag aagttcggcc tggagcggca cgtgtactcc gccaccaccc
     1621 gggtgatcgt ggtggacgtg gaggagaagc tggtcgggat ggtggtggac gccgcccgcc
     1681 aggtggtgcg ggtccccgcg gaccagatcg atccgcctcc ggccatcgtc ggcgggatgt
     1741 ccacggacta catcaagggc gtgggcaagc tcgatgaacg gatgatcatc ctgctcaata
     1801 tcgaccggat cctcagcagg gaggagaagc tgcagctcca gaagatggcc ggcgcctcgg
     1861 agggcgccgc ggagatggag ccggcgcttc aggcctgatg agctcgagcg gcggtcccgt
     1921 ccggggccgc cgtccatggg gcgttccaga gatcaggaag gcagtggttc tgacatgagt
     1981 acggtcatcg cctcggggga gtcggatcgc ggggcgacgc tcaccccggc cgcggtacct
     2041 tcggcgccgg cggcggcttc gcaagcgtcg gccgcgacat ctccggcaaa gggccccgcc
     2101 actgaaacgg agctggggga agaggcgcgg ctgaccgtcc accgcgtgct ggaggcgttc
     2161 gacgcgtcga aaacgggtct ggaatccctc cacaaggacc tcttccatcc aagcccccgg
     2221 gtggtgacct cggcgctgag cgccctcagc aagctgcggg atcagcgtag catgcgattc
     2281 gtggcacgcc tgctcacgag cgccaacgag cagatccgct gcgccgcggt gaggaccacc
     2341 gcggagatcc gtcaccctca ggccgctcgc atcctgttcg acctgctcaa gatcaccacc
     2401 ggggacgcca cccggcgggc tgccctggaa gcccttgcgg tcgtcgcgcc cgagactcct
     2461 cagctgcgcg ccagcctcca ggagtattcg cggtccagca cggtctcggg ctcgacccgc
     2521 gcgctggcga cgggactgct gctgcgcacc gggggcctga aggagcctgg ggacctcctg
     2581 gactccgccg aagaggaagt gatcgagagc ctcttcaccc aggcgcagac cagcccggag
     2641 atcgccgacc acgtggtccg ggacgggatc tcccactacc accgtctctc ggcgaagcac
     2701 cgcgctctgc tgctctccct ggccgcgccc ttcacggtgc cgggctccgc ggacctcctt
     2761 ctgtgggcgg tgagcgacgc ggaccaggac gtgcggagag ccgcctacca gctcctggca
     2821 cagccttcga cgaagccgtc caacctggag acgatcctca agaccattgc ggacagcgtg
     2881 gatcccaccc cggccctgga ggacgaggcc atcggcgccc tgcagcggat ggagccgcgc
     2941 gaagagcaga gcgccatggg ggagaagctc ttgggccgca tccaggagct gttccagacc
     3001 gtgagtcgcg aagaccgacg cgtcgtgagc gacagtcacg agctgggatg gctgatctcg
     3061 cggtccaagg agtacgtgga gtactacctg gacgaggacg tccgccacgc cctggtgcag
     3121 tacctgaagg gggtcggcgg ctacagcaag gcccgtctcc tccagtccct gcgggagacg
     3181 gcggtgcggg tggaggtccg gcacttcgac ggctaccgcg ccctggcgga cctcatcgag
     3241 gagcccaagc ggcacgggat cgcgctggtg gtccgtgagc tggccattgc ccgactgggc
     3301 cgccgaaagc ctttgagcct cctgatccgc aacctctgga tcagcaggct gctgaggacc
     3361 acgggctccc cggaggccca ggaacagctc ttcctggaga tctacacctg gagctcccag
     3421 gccaagattt tccggctttc cgaggccgct ctctttgcgc tgtcccgggt caacctggcg
     3481 aggacagcgc agctctgccg cgagtgcatg cggactcccg tggtgagcaa gctcctggcc
     3541 atcgcctccg ttcatctcct gaaggacctg gactggagct ccatggagtc ttcggtgctc
     3601 gggctcctta cgggcgcgga tgacacgaac gtcctgctca acctgttcga ggcgcttgcc
     3661 agcagcgatc gcagcctgaa cggggagatc ctcaagacgc ttctgggcgt ggttcgcctg
     3721 ggccccaacc ggcaggtcgt ccaggaggcg gcggagctcc tggcgggcca gccgggatcg
     3781 gaggcgctgg acgcggtcaa ggaggccttc gatcgcgcgg agccctggag acaggcgatc
     3841 ctgctctcca tcgtcgagcg cagggtccgc gaggggaacg tcagcaaccg cgaggggctg
     3901 atcgagttcc tgtacaaggt cctccgccag cagttcagct ctctggacgg gcgcgcggcc
     3961 atcctgctct ggcgtctggg agacgactac gctcagcagc tcctgcggga cctttcccgt
     4021 cgaggcggcc tggagcagcg cacggagctg gttcgcggcc tgaagggatt cgttaccgag
     4081 gaaatggcgg agctcctggc cccgctggcg cgcgtggaaa gctcgagcct gcaggaggtc
     4141 ctgcgggaca cgctcctggg cgcggaggat gccggcgccc agcggagaat ctgcgagctc
     4201 gtgctgacca cccaggaccc cagtgaggag gacgacgagg cggacgccgg ggacgtgcag
     4261 gtagacctgg cgaaggagaa gagagcctac cagttcgaga agacgttcat ccaggagctg
     4321 gcggtgcttt tcaccgacat cgtcggatac tccaagaagg cccagagcct cacctccatg
     4381 cagctcacct ccatgatcca ggactacgag ggcgtgctgc ttccaaccgt cagcacccac
     4441 cagggcgagc tgatcaagag gatgggcgac gggcacctct tcgtcttccg ttcccccttg
     4501 aacgcggtgc tggcggccat acgcgtccag aaggccctca agcggttcaa cagctaccgc
     4561 gaggagagca cgaggctggc cattcgggcc gggatccact ggggcaaggt agtccgcaaa
     4621 gagggggacg ttctcggcaa caacgtcaac atcgcctccc gcctggagtc caacgcccgc
     4681 ccgggctccg tcctgatttc tgatgccgtg caggcgatgc tggggggcca catccactgc
     4741 agggagatcg gcttcatccg cgtgaagaac atcgcggagc cgatcaaggt ccacgagcct
     4801 tacgagttgg tactggattt ccccgaggag ctggatcccc tcaaggcgct caagaaggac
     4861 gggggagagc attcggcgga ggaagctgcc ggagcggccg aaccgtccga gcagcccggc
     4921 gaagggacgc gaccgcctca gagcgccgcg cccgcccacg gcgatcccaa gcccgcggag
     4981 ggcaacgggc agcgcgcgac acgttccctg gacggagaga cggcgcggta cctgacagag
     5041 acgttcacct gtctcaacgc gatctgccgc gctgctgaag cgaaccaacg accgatgtcc
     5101 gacgtccgca aggagctggc tacgcgatgg gcgggtctgc gggagatgct gcagcgcccc
     5161 ggcgcgtgac cttgggcgcg tgaccctcgg ttgacggcag gccggcccgg gcccatacta
     5221 cccacgacgg gtccgcccgt tgcatccgtg ccttccccgg ggcgcccgcc ggaggaaggt
     5281 catgcagaac gtactgctag cctggttctc ggtgaacggc acgacaaccg ccatagccga
     5341 gcggatcgcc gcgggactcg cgaaggccgg ctgcgccgtc acccccattc gcatcggcaa
     5401 ggagaagcca ccggacctcg cggcgttcga cacggtgggg atcgggacgc ctacgtacgt
     5461 ctttcgaccg ccgttcgtgg tcacggattt tctacggtcc ctgccggatc tcacggggaa
     5521 acagtccttt gcattcgtcc tgcacgggac ggaccgaggc gcctgcgcca atcaggtgcg
     5581 aaagaccctg gcgcggaagc acgccaggga cctcgggctc taccactcct tcgggtccga
     5641 cctgttcaac ggctatctga ggcggggcgt gctcttttcg ccggacacgc cgagggcgca
     5701 agacctcgac gcggcggagc ggttcggtac gagcctggcg gagcgaggcc ccggtggggc
     5761 gcgcgtcccg gagccgatgg acgggcccac gcacctcatc tacgccatcg agcggctgtc
     5821 cgccagccgg gcgctcaccg ggctgctctg gagccgcgca ttccacgcgg acgagcggtg
     5881 cgacaactgc ggcgtctgca tcgccgcctg cccgatgcac aacatcacgg cccagaagga
     5941 tgcccgaccc cgttgggaga ctcattgcct cctgggcgaa cgagatggga ttccctttgt
     6001 cagggtcacc cactccggcg gggtcacgcg gcgtctgtag cgcgggcgtt tcggcgcggc
     6061 gactgcccat cgatcgctcg gagggagccg cgtggtttct tgaccgctac gctcgctggt
     6121 cgtattttga gtacttgggc agcctttggt ctcgagagca gacgctcggg gactcggctg
     6181 cgggagcgtg tcatggagag tcacaccgtc accggaggca aggatcccat ccgcctgttt
     6241 cggtccgatt tcctggagtt cttcacgcac gtgcatccgg cggtggtgct ggcggtgtgg
     6301 ctccccgtgg ccgccgcctt tctgacgtgg tccgcgctgc agagcgctcc cagggtgtgg
     6361 ctttccgtgc cggtttgctg ggtagcgggg ctggcgtcgt ggtccctggt agagtacgtc
     6421 ctgcatcggt tcattttcca cttccggccg cgaaccccgc gtcaggagcg actctccttt
     6481 ctggttcatg gggtgcacca cgcccagcct ctggtgaaga cccgtctggt catgccaccg
     6541 gtggtgagcg ttcccctggc tctcttcttc ttcggattgt cctggctcat tgggtcccgg
     6601 ctcctgggca agccggtctg ggctgattcc tactttgccg ggctcgtggt cggttatgtc
     6661 gcctacgaca tgctccacta ctccatgcat cattttcagc ttcgatcgcc gctgctctcc
     6721 gctctgcgga agcaccacat gcagcatcac gcggtgacat gggacaagcg gttcggagtc
     6781 tcctcccccg tctgggatca cgttttcggc accgagccct agggcaaccg gtccgaagcg
     6841 ccagggtccg ctgctttcgt ggcctcgctt ccgccgtggt acaatgaggt agtccacctt
     6901 gttcgcggaa ggagtcagcg atggccgatc cggactggga ttcacttacc caggagactg
     6961 tacggaatct gtcccggttg cttcaggcac agaccgccaa cccgccgggc aacgaagagc
     7021 ccgccgtaga ggtgatccgg cagatcctcg aggcaaacgg gtttccctcc cgtgacctcg
     7081 cgatcgtgga atccgcgccc catcgggtca acctggtggc gcggctcccc ggggatggct
     7141 ccctccggcc gttgctcctc tccgggcata ccgatgtagt gcccgtggaa cgggagcgct
     7201 gggcccggga tcctttcggg ggcgaggtcg tggatggcga gatctggggg cgcggcgccc
     7261 tggacatgaa aggctccctt gccatgtacc tggaggtctt cctgatggcc ctccgaagca
     7321 aggcgcgttt cagacgggac ctgatcctgg ccgcggtggc ggatgaggag gccgggttca
     7381 cgcatggctc gaagttcctc accgatcacc accgggagct gatcgaggcg gagtacggga
     7441 tcaccgaggc cggggcgatc acggtctacg tcggcaaggc ccgactgtac cccatccagg
     7501 ttgcggagcg gggcatatgc tggctcagga tgacggcgag cgggaagccc ggccacgggt
     7561 ccatgcctca tccggacaac gcgatcctgc atcttgccga ggccatcgaa aagatccgcc
     7621 gcgtccgcca cctccccgtc catctgacat cgacgttcct gaagatgatg gaggccgcga
     7681 gccgccaggt ggcctttccc ctgagtctcg taccgagcct gctccgcagt cccggactcg
     7741 ccgggttcct cctgaaccgg ctgccggagg agagccgcgg cctcctgtcg gccatggtcg
     7801 cgaacacggt gaccccgacc atcctcaaag cgggcgtcga tacgaacgtg atcccctcgg
     7861 tcgccgaagc gctgctggac tgccacaagc tcccgggcca gacctcgcag gacgtcatgc
     7921 gggagatcgg ggccatcacc ggaaacagcg taacgctgga ggtggtccgc gagtcgcccg
     7981 gcaccgagtt cccggtggat tcgcccttct accgcacgct ggaagccgcc gcccgccgca
     8041 tggacccaca agggatcttc gtgccgatgc tcatgccggc cgccaccgat gccagccact
     8101 accagcgcgc cggggtcagc gtctacggat tcactcccgg cgtgctgccg ccggagtttc
     8161 cgaccttcca gatcgggcac ggtcacaacg agcggctgcc ggtctcctcc atccgaagcg
     8221 gcttgccggc gctttgggag gtattgacca acacctgcgc gtaaggggat ccattgcggc
     8281 atgacgagtg agctgctgat cctgatctgc cagaccgcgg tggtatacct gctggtgctc
     8341 gggatgcact ccctgcgccg gcggacgggg ctgtccctct tctacgcgct gctgggcgga
     8401 ctgaccgcca tcatgtcctg ggtcacggac gccggtctct ccgtccacgt gggcggcgtc
     8461 accttcgtgg tcggatccac ggtcttctac accgccctgc tcctcggggt cttcgtcgtc
     8521 tacgtattcg acggccctct ctcggcgcgg accgcgatcc tcggcgtggc agtggtctcc
     8581 acgctgacac ccctggtttc cgccttcctg cacctccagg cgtgggcggc cggcagcagg
     8641 ctgcttccgt tcatccccac gccgagcctt cggatcaacg ccgcctcggt tgcggccacc
     8701 ctggtcgaca tggtgttcct ggccgtggcc tgggagtatc tgggacggcc gcgcatccac
     8761 cggtggctcc gcgtgtacct gaccctgctc ggtgtcctct gcctggatgt ggtcctcttc
     8821 aacacgggag ccttcgcggg caccggctcc ttcctgacga tcgtacaggg aaccctcatc
     8881 agccgggtct cgatgtccgt cgtggccctt cccttcctgc tggtctacct ggagtggcag
     8941 agccgatgga agggagaggc cattgagaac cgccccgtgc tcgcgatcct gcgccaggtg
     9001 gcgaagatcg aggcggagct gtcccgcgcc caggtcgaga tcgagttgcg gaaagccgcg
     9061 gaacggcaac gggatgagac catccgtgag ctgcgccagg cgctgtccga ggtgaagacg
     9121 ctgcgcggtc tcctgcccat ctgcagccgg tgcaaacgga tccgggacga cagcggctac
     9181 tggaatcgga tcgaaaccta catccgggac cacacggacg cgcgtttcac tcatgggatc
     9241 tgccccgact gcgcccggga gctgtacccg gacgtgccga tcgatccgga gtgaggtccc
     9301 ctcaggccca cttctctcgg atcgcccgga tcacctcgta gatctcgaag aagactttca
     9361 ccagctctgg gtcgaactgc cggccggact gctccttgat gtagaagaga acccgatcct
     9421 cttcccaggc gtccttgtag gcccgcttgc agatcagcgc atcgtagacg tcggccagcg
     9481 tgacgatccg cgcggagagc gggatcgcgg agcccttctt gccgggaccg aacttcagcc
     9541 ccttggcgtg gatgtctttc acgactcccg ggtacccgcc gccatcccag cgctcgtggt
     9601 ggttcagggc aacctcgtag gccagggtat cccattccga ggtggggttc tcgaagaact
     9661 gcgcaccgaa caccgtgtgg tacttgatgc tctcgtattc ctccgcggtc aggtcctcgc
     9721 gcttcttgag gatcgcgtcc gacacgccga cctttccgat gtcatggagc atcgaggaga
     9781 tgcgcaggat gtccttgaag ttggtgatct cctcccgcgg caccccatgc cgctccgccc
     9841 acttctggta cagctccacc gcgtaggagc cgacccggct cacgtggggc tgggtctcct
     9901 gcgggtcccg cagctccgtg gtgcggacca tcttgaacac cacgtcgcgc aggagcagcg
     9961 cgcgctcgat ggcgatcccc gcatagaagg cgaactgggt gatgtagaac ctctggtccg
    10021 agctgaaggg gataggatcc ccggcggagg aaagacggtt gatcagctcc aggacgccca
    10081 ccatgttgtt ggtgctcgtg cggatcggga cgatgagcat ggatacagtg cggtacgagg
    10141 agatctcgtc gaaatagggg ttgaaggaga acgggtagtc ctggtcgagc tggtaggcgt
    10201 cctcgatcat cagggactct cccgtggacg cgacatagcc ggcgatcgac ttctcgtcga
    10261 tgtcgatcga gttgttcgtg tagatgtact tgttcgagag gaagtctttc ttgaagagcg
    10321 agtcgttctg cacgtagctg aagagcagct tcttcccttc caccaggaag atggagccgg
    10381 catccgcgtc cgtcagggac cgggtctcct gaaggacgct gtccaggagc gtgttcaggt
    10441 cccgaatgtg gtggagccgt tcctgtttct ccaggatcgt attgaggagg gtggcattgt
    10501 cccgggtccg ccggacggtc cccgccttct tcatggccgt tgcacgccgt tgacaccgag
    10561 gaagatggag tacagggacg tgaagccggt gatgaagagc cggttgcgct tgggaccgcc
    10621 gaaggctacg ttggataccg cctcggggat cgggatccgg accaccaggt ctcccgcggg
    10681 cgtgtagcag tgcacgccgg cgccggcgct ggtccagatg ttcccgtcgg tgtccaggcg
    10741 gaacccgtcc gagcgcccct ggcgcatgtc cgcgaagacc cgcccgccgg tcagggtctt
    10801 gccgtccttc acgctgaaag cgcggatgtg gggcggtccc ccgggccggt cggcatggct
    10861 tgtgtcggag acgtacagga tcgattcgtc cggggagaat gccagcccgt tgggccgctc
    10921 gaagtcgtcg gccaccgcgg caagggtacc ggtggaaggg tccagccgga agacgtagca
    10981 ccggtcctgc tcctgcgctg acttgtgccc ctcgtagtcc gtcaggatcc cgtagggcgg
    11041 atcggagaac cagatgctcc catcggactt caccaccacg tcgttcggag agttcagcct
    11101 cttaccgcgg taggcgtccg ccaggaccgt gacggtcccg tccggctcgg ttcgggtcac
    11161 gcgccgcgtt ccgtgctcgc aggtgaccag gcgcccttgc cggtcccggg tgttcccgtt
    11221 cgcgttgttg gacggggatc gaaacaccgt gacgggagcg ccttcggacc agcgcagcat
    11281 ccggttgttg gggatatcgc tgaacaggag acagtcgagg tccgcgaaaa acaccgggcc
    11341 ttccgtccac cgcgccccgg tccagagctt ctccaggaag gagttgctcg gaaccaggct
    11401 gccgtacttg gagtcgagaa tctcgaagtc cgccatggaa cctcctggct tcgcacggtg
    11461 ctccgtgcgg cctgatcgac gggcgcgggc cgctcaaccg cagccgcgcg ccggctcgat
    11521 cacggtatca tccccggcgg ctatcggcca gaggcgctca tcgaggcacc cttccaaccg
    11581 ag
//
LOCUS       DASRZS010000204         3453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2038540, whole genome shotgun sequence.
ACCESSION   DASRZS010000204 DASRZS010000000
VERSION     DASRZS010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2038540"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VFH83_05135"
     CDS             <1..1170
                     /locus_tag="VFH83_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6485778.1"
                     /translation="ELVPGIAGGSGTEQAAIPERGSFLAASPWRRIVIAVFGPLFNAV
                     FAILAFTVIWWAGFAIPSSDNRVILASDYTLDSFATPPPATVAGLRTGDRITAINGVP
                     VEKFQDIVETVSVAPGKRFLFTVQRDVQGASQVLTLPIAPELDRSSGAGRIGIYAWVD
                     PVVDTVAPNSSAAVAGLRKGDRLISVDGKPVLNTIDLEQALQGKPAKLAVELDRGGER
                     LNDTLVVNYDEKGSTNLGIGFASKIYHSLRVGPGGAFVHSLEETGNTIGLTLKGFGLL
                     FRGIQLRNAVAGPLRITYYIGSAAASGLQMGLGAGVVAFFRLLSFLSVVLFLMNLLPI
                     PAMDGGQVVLFLVEVARGKPVRSQLIWRLQLLGFSLLILLSLMVTFSDILFFAGR"
     gene            1174..1818
                     /locus_tag="VFH83_05140"
     CDS             1174..1818
                     /locus_tag="VFH83_05140"
                     /inference="COORDINATES: protein motif:HMM:NF025711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HET6485779.1"
                     /translation="MDLQKRRIACFCENSFDAEVPKSADLAADPQVEEQILNGDFMAV
                     TCPACGKRLTPEFPFHLVGRGSPLRPQGWDAFLVPEEDRVAFLRGKLDYAPGTPTRVV
                     VGFPELAEKVLVFGQGLDDRVIEVMKYYLLTGSAARSEAEMDPVFSYHGQEGGRHVFH
                     VSGLRKGEVAVARLGTELYQKIAADIERRVTEDPFKDFCAPPWVSLRKVSGEPQ"
     gene            1815..>3453
                     /locus_tag="VFH83_05145"
     CDS             1815..>3453
                     /locus_tag="VFH83_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485780.1"
                     /translation="MIRRVLALLGAALLIACPTFPTGTAESGVRSVVTTGHTGAVLGL
                     RYDSGRHMLFSSGDDGTVRVWDMDSGALAHCLQVTRSNTRAMAIDPSSARLAVVVTDG
                     IRSFALSVWDWNEERQLYRVALTEEPQFLRFSPKGTYLLYGTSSWQGLHLLNASDGTQ
                     RAFHPEGFGIVAFANVSDAEDALLSYQVSGKLSSWDLATGDLTREIRTVPYLSMARIS
                     ASGQVLAGYTGQSLVAIDTQTGSSLGQVAVPSVSSLDISAAGDELATVAAGKLTRWAI
                     ASGAIRPAARQPQLTASAPAPTQVCYGGDGLFVGDGSGEVRGVPTSGAERLIGKNELS
                     SVSGFDARGGMLAVASSDWIRVFCGDTAPAGGSDQAPGFVYSMECPNPFRGPVGLTFV
                     SPTRLLAWRRDGAPPILAFVDIPQSGASLPEKGAFRTLTSAFRAPLTSFHLLGSLAVG
                     VESTGTVRCVDLSTGSSIFDMRVPGVSSAVPVSPTAVLAGRSAVLATDSSLLRIDITT
                     GETVGVKDPNVFTYGLEDDLGTAAGSGAAAGSGSTPAS"
BASE COUNT          489 a         1155 c         1190 g          619 t
ORIGIN      
        1 gagctggtcc cggggatcgc cgggggcagc gggacggagc aggccgcgat cccggagaga
       61 ggctcgttcc tggccgcctc cccctggaga cggatcgtca tcgcggtgtt tggaccgctg
      121 ttcaacgccg tcttcgcgat cctcgccttc accgtcatct ggtgggcggg gtttgccatt
      181 ccttccagcg acaatcgggt gatcctggcc tcggactaca cgctggattc cttcgccacg
      241 ccgccgccgg ccaccgtggc ggggctccgg acgggagacc ggataaccgc gatcaacggt
      301 gtccccgtcg agaagttcca ggatatcgtg gagaccgtct cggttgcgcc gggaaaacgg
      361 ttcctgttca cggtgcagcg cgacgtgcaa ggcgcgtccc aggtgctgac cctgccgatc
      421 gccccggagc tggaccgatc ctccggcgct ggccggatcg ggatctacgc gtgggtggat
      481 cccgtcgtgg atacggtggc ccccaactcc tccgcggcgg tggcaggcct gcgcaagggg
      541 gaccgtctca tcagcgtgga cgggaagccg gtgctcaata cgatcgacct ggagcaggcg
      601 ctccagggca agccggccaa actggcggtg gagctcgatc ggggagggga gcggctgaac
      661 gacacgttgg tcgtgaacta cgacgagaag ggctcgacca acctcggcat cggcttcgcg
      721 tcgaagatct accattctct gcgcgttggt ccgggcggcg ccttcgtgca cagcctggag
      781 gagacgggga acacgatcgg gctcacgctg aaagggttcg gcctgctctt ccgaggcatc
      841 cagcttcgaa acgccgtggc cggtccgctg cggatcacct actacatcgg gagcgcggcg
      901 gccagcggac ttcagatggg gctcggcgcc ggcgtggtcg cgtttttccg cctcctctcc
      961 ttcctgagcg tcgtcctctt tctgatgaac ctgctcccca tccccgccat ggacggggga
     1021 caggtcgtcc tcttcctggt tgaggtggcg cgggggaagc cggtgcgctc ccagctcatc
     1081 tggcgcctgc agctcctcgg gttctccctg ctcatcctgt tgagcctcat ggttaccttc
     1141 agcgacatcc tcttcttcgc aggacggtga gtcgtggatc tccaaaagag gcgcatcgcg
     1201 tgcttctgcg aaaacagctt cgatgccgag gttccgaagt ccgcggacct ggcagcggat
     1261 ccccaggtgg aggagcagat cctcaacggg gacttcatgg ccgtgacctg cccggcctgc
     1321 gggaagcggc tcacgccgga gttcccgttc cacctcgtcg gccgcgggtc gccgctgcgg
     1381 ccccaggggt gggacgcctt cctggtgcca gaggaggatc gggtcgcgtt tctgcgcgga
     1441 aagctcgatt acgcgccagg gacccccacc cgcgtggtgg tcggttttcc cgagctcgcg
     1501 gagaaggtgc tggtcttcgg gcagggtctg gacgaccggg tgatcgaggt catgaagtac
     1561 tacctcctca ccgggtcggc ggcgcgctcc gaagcggaga tggatccggt cttctcctac
     1621 cacgggcagg agggggggcg gcacgtgttc cacgtctcgg ggttgcgaaa gggagaggtg
     1681 gcggtggcgc ggctggggac cgagctctac cagaagatcg cggcggatat cgagcgcagg
     1741 gtcacggagg atccgttcaa ggacttctgc gctcccccct gggtgtccct tcgaaaggtc
     1801 tcgggggagc cgcagtgatc cgccgggttc tcgctctgct cggcgccgcc ctcctgatcg
     1861 cctgcccgac gttccccacg ggcacggcgg agtccggggt caggagcgtg gtgaccaccg
     1921 gccatacggg cgcggtgctg gggctgcggt atgacagcgg gcgacacatg ctcttcagct
     1981 ccggcgatga cgggaccgtt cgcgtctggg acatggattc cggcgccctg gcccactgcc
     2041 tccaggtgac ccgctccaat acgcgggcca tggccatcga cccatcatcg gcgcgactgg
     2101 cggtcgtggt gacggacggc attcggtcct tcgcgctctc cgtctgggac tggaacgagg
     2161 agcgacagct ctatcgggtg gcgctgaccg aggagccgca gttccttcgg ttctccccca
     2221 agggcaccta tctcctgtac gggacctcct cctggcaggg cctgcacctc ctgaacgcct
     2281 ccgacggaac gcagcgtgcc ttccatccgg aagggttcgg gatcgtggcg ttcgccaacg
     2341 tcagcgacgc ggaggacgcg cttctctcct accaggtctc cggcaagcta tcctcgtggg
     2401 acctcgccac gggggatctg acccgggaga tccgcaccgt tccgtatctc tccatggccc
     2461 gaatctcggc aagcgggcag gtgctggccg ggtacacggg tcagtccctc gtcgccatcg
     2521 acacgcagac gggctcttcc ctgggccagg tagccgtacc ctcggtcagc tcccttgaca
     2581 tatccgccgc cggcgacgag ctggctacgg tggccgcggg gaagctcacg cgatgggcca
     2641 tcgcgagcgg ggccatccgt cccgccgcgc ggcagccgca gctcaccgcg agcgcgccgg
     2701 cgcccacgca ggtctgctac ggcggggacg gcctcttcgt gggagacggg agcggtgagg
     2761 tccgcggagt tccgacgagc ggcgccgagc ggctgatcgg gaagaacgag ctctccagcg
     2821 tctcggggtt cgacgctcga ggcgggatgc tggcggtggc ttcctcggac tggattcggg
     2881 tcttctgcgg tgacacggct cccgcgggcg gctcggacca ggcgccgggc ttcgtctatt
     2941 ccatggaatg cccaaacccg tttcggggcc ccgtggggct gaccttcgtg tcgcccaccc
     3001 gcctgcttgc ctggcggcgc gacggcgccc cgccgatcct tgccttcgtg gacatccccc
     3061 agtccggcgc ctcgctcccc gagaagggag cgttccgcac cctgacaagc gccttccgcg
     3121 ctcccctcac gtcgttccac ctcctgggat ccctcgccgt cggcgtggag tcgacgggaa
     3181 ccgtgcgctg cgtggacctg tcgacgggct cgtcgatctt cgatatgcgg gtaccggggg
     3241 tctcgtcggc ggttcccgtt tctccgaccg ctgtcctggc aggccgatcg gcggtcctgg
     3301 ccacggacag ctcccttctg cggatcgaca tcaccacggg tgagaccgtc ggcgtcaagg
     3361 atccgaacgt tttcacgtat ggtctggagg acgatctggg cacggccgct ggctcgggag
     3421 cggctgccgg gtccggcagc acaccggcct cca
//
LOCUS       DASRZS010000205         7882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1266382, whole genome shotgun sequence.
ACCESSION   DASRZS010000205 DASRZS010000000
VERSION     DASRZS010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266382"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..600)
                     /locus_tag="VFH83_05150"
     CDS             complement(181..600)
                     /locus_tag="VFH83_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485781.1"
                     /translation="MKRFAVAFALVSALSIVLAAAAFADTNSDLANAVKAVLDQSKPT
                     GWLADAQVGEQPNQIYTDAQGLVGQPVDMLTKEMGSAQTMSDGKGGMWYYYKIDLTNS
                     TESMYSASANAAPNHSAFEWFDVGSDGTVASDSVSFG"
     gene            920..2425
                     /locus_tag="VFH83_05155"
     CDS             920..2425
                     /locus_tag="VFH83_05155"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET6485782.1"
                     /translation="MAILYPEPSHTFGEYLLIPNLTRKDCTPDQVRLDAPLVRFRDGE
                     TSAIRLNIPFASAIMQAVSDDAMAIALARSGGISFIFVSQPITRQAEMVERVKRFKAG
                     FVVSDSNIRPEATLQEVLALRERTGHTTMAVTADGTPNGRLLGILTSRDYRLSSTALT
                     ERVGDLMTRLPDLIYGKEGISLSEANDLIWEHKLNCLPIVAPDGTLAQLVFRKDYAEH
                     KENPRELVDGAKRLVVGAGINTRDYRERVPALLAAGADALCIDSSDGYSEWQADTIQW
                     IHKTYGDAVKVGGGNVVDAEGFRFLADAGADFVKVGIGGGSICITREQKGIGRGQATA
                     VIEVAKARDEYHRETGIYVPICSDGGVVLDYHITLALAMGADFVMMGRFFARFDEAPG
                     RRLRVGGTFVKEYWGEGSNRARNWQRYQHNGQSLAFEEGVDAYVPYAGKLKDNIDSTL
                     DKIRSAMVSCGAVSVCELQKKARITLASAVSIREGGAHDIQLKENEILPTS"
     gene            2433..3131
                     /locus_tag="VFH83_05160"
     CDS             2433..3131
                     /locus_tag="VFH83_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HET6485783.1"
                     /translation="MKPTLLDCGPVLVDGRQVAERLGLSPDPDLEAELDSLAAQASRV
                     ARPRAVYADAFVEERTEDETVIGGIRFRSRVLGVNLRDVHRVFPFVVTSGLEVEAWSQ
                     EITDPLMRFHADTVKELVLYHAAERLAAHIDHAFALKGATAMNPGSLEDWPLSEQAPL
                     FRLLGDVKAMIGVTLTESFLMHPVKSVSGIRFPAEASFESCMLCPRESCPNRRAAYDP
                     QLFQTRYQGAVGHG"
     gene            3124..4260
                     /locus_tag="VFH83_05165"
     CDS             3124..4260
                     /locus_tag="VFH83_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HET6485784.1"
                     /translation="MDEGRGAAPAADFGSWQEQLANSIRTEGELEAFIRLTPAERDGI
                     RLAGARYRWGITPYYAGLMDPIDPTCPIRRQVIPSIEELSDDAGTADPIGEEAGSVTP
                     TLIRLYPDRVAWRVSGVCSAYCRFCFRRRLASDPPEDFSERALQTALDWIGTTPEIRD
                     VLITGGDPFLLGDDRLRDLLGRLRAIEHVEVIRIGTRTPVTLPHRITGQLCEMLRAFH
                     PLWINTHFNHPKELTPLAISACARLADAGIPLGNQSVLLRGVNDDEPTMRRLVHGLVK
                     ARIRPYYLFQCHLNRGTAHFRTSVEQGRDIVGALRGSTSGFAVPTFVVDTPYGKVPIQ
                     PQTILGREGDAILLRAWDGRVWREPNRAPGSEPDARDAQGRPDA"
     gene            complement(4257..5024)
                     /locus_tag="VFH83_05170"
     CDS             complement(4257..5024)
                     /locus_tag="VFH83_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET6485785.1"
                     /translation="MVRLISWNVNGIRAAIAKGGLSAVARQRPDVVCFQEVRAAPDQI
                     GRVLRPLSFRCHAIPRRAGYSGTAIFSRVEPIRWFAGIGERTSDAEGRVVTAEFPSLY
                     VVSVYVPNSQRGLPRLGYRLRWDARFRKFAAELAAAKPVLFCGDLNVAHREVDIARPR
                     ANRMNAGFTDRERRSFSRLLSTGFVDSFRHLHPDETARYSWWSFATNARERNIGWRID
                     YVCLSSAAAPRLRSAFILDRIRGSDHCPVGVELEGEL"
     gene            complement(5033..5800)
                     /locus_tag="VFH83_05175"
     CDS             complement(5033..5800)
                     /locus_tag="VFH83_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HET6485786.1"
                     /translation="MGLMSGKRGVIFGVANDKSIAWGISRRLHEEGASLAFTYLNEVF
                     EKRVRPLAESIGSELILRCDVSKDAEIEEVFTEIEQTWGSLDFLVHAVAYAGREELRQ
                     PFHQTSRDGFRQALDISAYSLVAVTRAALPLMKNGGSILTLTYLGSQRAVPNYNVMGV
                     AKAALEATVRYLAAELGEKAVRVNAISAGPIRTLAASGISNFSEIFGIMESRSPMHRS
                     VSQEEVGNSALYLLSELASGVTGDIHFVDTGYNIVGL"
     gene            5916..6620
                     /locus_tag="VFH83_05180"
     CDS             5916..6620
                     /locus_tag="VFH83_05180"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET6485787.1"
                     /translation="MSQPPSSEIARGLRAGIPIAVGYVPIAVAFGFLASQGRLSLAQA
                     AAMSLFVYAGASQFAAVGMLAGGASALAITLATLVLNFRHFLMSVSLSRRISEGGSPR
                     RSWVPALALGVTDETFVVASTDGAPTAPYFLGLGLMAYCAWNAGTLIGAGFSALIPPG
                     LAHGLGITLYAMFIAILLPGIRKSWVNGIVAVAGGFLAWCASVILPGLPGGWRIVIAI
                     IVASALGAALGGEDAA"
     gene            6617..6919
                     /locus_tag="VFH83_05185"
     CDS             6617..6919
                     /locus_tag="VFH83_05185"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET6485788.1"
                     /translation="MIWLTIAGMALVTYVPRLIPMALGRDLPLPTWIRRWLSFFPYAA
                     LGALIFPGILTAIQGRPWVSLAAGATAALVALRAKNAIIPVLAAVAVALALGAVVR"
     gene            7012..>7882
                     /locus_tag="VFH83_05190"
     CDS             7012..>7882
                     /locus_tag="VFH83_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET6485789.1"
                     /translation="MALLGGSTKIFVLDTNVILHDHDCINSFEEHNVVIPITVLEEID
                     RFKKGNEIINFNAREFSRKLDSMGGEQLLNEGIHLKSGGMVIVETNVRPDGLLKEVFW
                     EDKPDHRILSLAFNLAQEHGRDRVILVSKDINARMKAKGIGLRAEDYETGKIGNIDEL
                     YKGKQILEEVEGGLIDELFQEGVLARERLSLARDPYPNEYFVIRNSHKSILAVYDSQQ
                     GGFRHIQKKRAYGIEPRNAEQTFSLDALFDDDISLVTLAGKAGTGKTLMAMAAALEKR
                     SSYKQIYLARPIVP"
BASE COUNT         1353 a         2614 c         2580 g         1335 t
ORIGIN      
        1 tggaacggca tgggcaccct gaaggtgtac tgggacggct ccttcgcgtc gaccagctcg
       61 aacacgatcg agatcaactt tatccacaac ttctaggact atcgtgcatg aagaaagccg
      121 cccgaacgag ggcggctttc ttttgtcttg gcggggagat gcggcgctcc cgcgtcgagc
      181 tcaaccaaag gaaaccgagt cgctggcgac cgtcccatcc gaaccgacgt cgaaccactc
      241 gaacgcgctg tggttgggcg cggcattggc cgacgcggag tacatggatt cggtcgagtt
      301 ggtcaggtcg atcttgtagt agtaccacat tccgcccttg ccatccgaca tcgtctgagc
      361 ggatcccatc tccttggtca gcatatccac cggctgaccc acgagcccct gggcatcggt
      421 atagatctgg ttgggctgct cccctacctg ggcatccgcc agccacccgg tgggcttgct
      481 ctgatcgagc accgccttca ccgcgttcgc caggtcacta ttcgtgtccg cgaacgcggc
      541 agcggccagg acgatcgaga gagcggaaac gagggcaaat gcaactgcga atcgtttcat
      601 cgaatgtatc ctcctgcaag tcgaatcccg tgttccaccc aacgggtgac aatagtaggg
      661 taccggtcgg aggagggcgc attccattac atggcataaa acttccatga aagaactgtt
      721 atcaaatgaa gggtgttatg aaatgaagct tgttatgaaa tgaagaggcg ggccggttcg
      781 ccatgcgccc gatcggccca ggcatggacg gccgtttcgg catacctacg ggctcgcgcc
      841 cttgatcact gcggtccttt cgcgatagtc ttccttggca tgagagtcac cactacgagg
      901 acccgcgcgg tgaacggcca tggccatctt gtatcctgag ccctcccaca ccttcggcga
      961 atacctgctg atcccgaacc tgactcgcaa ggactgcacg ccggaccagg tccggctgga
     1021 tgccccgctg gtccggttcc gggatgggga gacctccgcc atccggctca acattccgtt
     1081 cgcctccgcc atcatgcagg cggtctccga cgatgccatg gccatcgccc tggctcgatc
     1141 gggaggaatc tcgttcatct tcgtttcgca gcccatcacg cggcaggcgg agatggtgga
     1201 gcgggtgaag cgcttcaagg cggggttcgt ggtgagtgac tccaacatcc ggcccgaggc
     1261 gaccctccag gaggtcctgg cgctgaggga gcgcacgggg cacaccacca tggcggtcac
     1321 cgcggatggg acgcccaacg ggcgcctgct gggcatcctc accagccgtg actaccgtct
     1381 ctcttccacg gccctcacgg agcgtgtcgg ggacctgatg acccggctgc cggatctcat
     1441 ctatggaaaa gagggcatca gcctctccga ggccaacgac ctgatctggg agcacaagct
     1501 caactgtctg cccatcgtgg ccccggacgg gaccctggcg cagctcgtct tccgcaagga
     1561 ctacgccgag cacaaggaga acccgcggga gctggtggac ggcgccaagc gcctggtggt
     1621 aggcgccggc atcaacacgc gggactaccg cgaacgcgtg cccgcgctcc tggccgccgg
     1681 agcggatgcg ttgtgcatcg actcctccga cggctattcc gagtggcagg cagacacgat
     1741 ccagtggatc cacaagacct atggcgatgc ggtgaaggtg ggcggcggca acgtggtgga
     1801 cgcggagggc ttccgtttcc tggcggatgc gggggcggac ttcgtgaagg tcggcatcgg
     1861 cggcgggtcc atctgcatta cgcgggaaca gaagggaatc ggccggggcc aggcgaccgc
     1921 ggtgatcgag gtggcgaaag cccgggacga ataccatcgg gagaccggga tctacgtccc
     1981 catctgttcc gacggaggag tggtgctgga ctaccacatc acgcttgctc tcgccatggg
     2041 cgcggacttc gtgatgatgg gccggttctt tgcccggttc gacgaggctc ccggccgccg
     2101 cctgcgcgtg ggcggaacct tcgtcaagga gtactggggc gaggggtcca accgcgcgcg
     2161 caactggcag cgctaccagc acaacggcca gtccctggcc ttcgaggagg gcgtggacgc
     2221 ctacgtcccc tacgcgggca agctcaagga caacatcgac tcgaccctgg acaagatccg
     2281 aagcgccatg gtgagctgcg gcgccgtctc ggtctgcgag ctgcagaaga aggctcgcat
     2341 caccctggca tccgccgtga gcatccgcga gggcggcgcc cacgacatcc agctcaagga
     2401 gaacgagatc ctcccgacct cgtgacatcg gcatgaaacc cacccttctg gattgcggcc
     2461 ccgttctggt ggacgggcgg caggttgcgg agcgtctggg gctctcgccg gacccggacc
     2521 tggaggcgga gctcgattcg cttgccgccc aggcaagccg cgtggctcgg cctcgagcgg
     2581 tctatgccga cgccttcgtg gaagagcgga ccgaggatga gacggtcatc ggcgggatcc
     2641 gctttcgcag ccgggtgctg ggcgtgaacc tgcgggacgt ccaccgcgtg ttccccttcg
     2701 tggtgacctc cgggttggag gtggaggcct ggagccagga gatcaccgac ccgctgatgc
     2761 ggttccacgc ggacacggtg aaggagctgg tgctttacca tgccgcggag cggctggccg
     2821 cccacatcga ccatgccttc gcgctcaagg gcgcgaccgc gatgaaccca ggatccctgg
     2881 aggactggcc cctctccgag caggccccgc tcttccgtct cctgggggac gtgaaggcca
     2941 tgatcggagt taccctgacg gagagcttcc tgatgcatcc ggtgaagtcc gtctccggca
     3001 tccgcttccc cgcggaggcc agcttcgaaa gctgcatgct ctgcccccgg gagagctgcc
     3061 ccaaccgtcg agcggcgtat gatccgcagc tcttccagac ccgttatcag ggggccgtgg
     3121 ggcatggatg aggggcgggg cgctgcgccg gccgcggact tcgggtcctg gcaggagcag
     3181 ctcgccaaca gcatccgcac cgaaggagag cttgaggcat tcatccggct cacgcccgcg
     3241 gaacgggacg ggattcggct ggcgggcgct cggtaccgct ggggcatcac cccctactac
     3301 gccgggctca tggatcccat cgatccgacg tgccccatac gccgccaggt catcccatcc
     3361 atagaggagc tctccgacga tgccggaacc gcggacccca tcggagaaga agccggcagc
     3421 gtgaccccga cgctcatccg cctctatccg gaccgggtgg cgtggcgtgt ctccggcgtg
     3481 tgctccgctt attgtcggtt ctgcttccga cgccggctcg cctccgaccc gccggaggac
     3541 ttctccgagc gcgcgctcca gaccgccctg gactggatcg ggaccacgcc ggagattcgg
     3601 gacgtcctga tcaccggcgg ggacccattc ctcctgggcg acgatcgtct gagggacctt
     3661 cttggcaggc ttcgcgccat cgagcacgtg gaggtcatcc ggatcgggac ccggacgccg
     3721 gtcacgcttc cccatcggat cacgggtcag ctctgcgaga tgctgcgggc gttccatccg
     3781 ctttggatca acacccattt caaccacccc aaggagctga ccccactggc catttccgcg
     3841 tgcgcccggc tggcggacgc gggcattccc ctggggaacc agtcggtcct tctacgcggc
     3901 gtgaacgacg acgagcccac catgcggcgc ctggtgcacg gactggtcaa ggcgcgcatc
     3961 cgtccgtact acctcttcca gtgccacctg aatcggggca ccgcccactt ccgcacctca
     4021 gtggaacagg gacgggacat cgtgggagcg cttcggggca gcaccagcgg gttcgccgtc
     4081 cccacgttcg tcgtcgatac cccgtacggg aaggtcccga tccagccgca gaccatcctg
     4141 ggccgcgagg gggacgccat cctgctcagg gcctgggacg gccgggtctg gcgggagccg
     4201 aatcgagcgc cgggttccga gccagacgct cgggacgcgc agggccgtcc ggacgcctaa
     4261 agctcgccct ccagctccac ccccacgggg cagtgatccg accctcggat ccgatccagg
     4321 atgaacgcgc ttcgaaggcg aggcgccgcc gcactggaga ggcacacgta atcgatgcgc
     4381 cagccgatgt tccgctcgcg cgcgttcgtc gcgaagctcc accagctata gcgcgccgtc
     4441 tcgtccggat gaaggtggcg aaagctgtcg acgaacccgg tggacagcag tcgggagaag
     4501 cttcgccgtt cccgatcggt gaagccggca ttcatccgat tggctcgggg tcgggcgatg
     4561 tccacctccc ggtgcgcgac gttcaggtcc ccgcagaaga gcacgggctt ggcggccgcg
     4621 agctcggcgg cgaacttccg aaaccgtgca tcccaccgca ggcggtagcc gagccgggga
     4681 aggccccgct ggctgttggg cacgtacacg ctcaccacgt acagcgaggg gaactccgcc
     4741 gtcaccacgc ggccctcggc gtcggacgtc cgctcaccga tgccggcgaa ccatcggatg
     4801 ggctcgaccc gggagaagat ggcggtgccg gaatacccgg cccgacgcgg tatggcgtgg
     4861 caacggaacg acagcggtct caggacccgg ccgatctggt cgggcgcggc ccgcacctcc
     4921 tggaaacaga ccacgtctgg tcgctgtctg gccacggcgg agaggccacc cttggcgatg
     4981 gcggcccgga tcccgttgac gttccaggag ataagccgaa ccacggagag ggctacaggc
     5041 ccacgatgtt gtagccggtg tctacgaagt ggatgtcccc ggtcacgccg cttgccagtt
     5101 cggagagcag gtacagggcg gagttcccga cctcctcctg ggatacggac cggtgcatgg
     5161 gggatcggct ctccatgatc ccgaagatct cgctgaagtt ggagatgccg gaggcggcca
     5221 gggtccggat gggaccggcg gagatcgcgt tcacgcgaac ggccttctcc cccagctccg
     5281 cggccaggta gcggacggta gcctccagcg cggcctttgc cacgcccata acgttgtagt
     5341 tgggcaccgc ccgctgcgag ccgaggtacg tcagggtgag gatggagcct ccgttcttca
     5401 tgagcggaag cgcggcgcgg gtgaccgcca ccagggagta agcgctgatg tccagcgcct
     5461 ggcggaaacc gtcacggctg gtctggtgga agggctggcg gagctcctcc cggccggcgt
     5521 aggccaccgc gtgcaccagg aagtccaggc tcccccaggt ctgctcgatc tcggtgaaga
     5581 cctcctcgat ctccgcgtcc ttggagacgt cgcaccgcag gatgagctcg gagccgatgc
     5641 tctccgccag cggccgtacg cgcttctcga agacctcgtt caggtacgta aaggccagcg
     5701 aggctccctc ctcgtgcaga cgtcgggaga tcccccaggc gatgctcttg tcgttggcga
     5761 ccccaaagat cacgccccgc ttgccgctca tcaggcccac gcgcataccc tccgccagga
     5821 ttgttggtag gagtatagcg accctccccg tgaggagcaa cgaaggacgg cccggtctgg
     5881 aacagaacgc cgggcccggg ttactatgcc cccgcatgag ccaaccccca tcctcggaga
     5941 tcgcgcgcgg gctgcgcgcg gggataccca ttgccgtggg gtacgttccc atcgccgtcg
     6001 cgttcgggtt tctggcctcg cagggaaggc tgagccttgc ccaggccgcg gccatgtccc
     6061 tcttcgtcta cgccggcgcc agtcagttcg cggcagtcgg gatgctggcg ggcggcgcat
     6121 cggcgctggc catcacgctg gccaccctgg tgctgaactt ccgccacttc ctgatgagcg
     6181 tctctctatc ccgccgaatc tcggaaggcg gatctccccg tcgctcctgg gtgccggcgc
     6241 tcgccctcgg cgtgaccgac gagacgttcg tggtggccag cacggatggc gcgccgaccg
     6301 ccccctattt cctgggtctg ggcctcatgg cgtactgcgc ctggaacgcg gggacgctga
     6361 tcggcgccgg cttctcggct ctgatccctc ccggcctggc ccacgggctg gggatcacgc
     6421 tctacgcgat gttcattgcg atcctcctgc ccggcatccg caagtcctgg gtgaacggga
     6481 tcgttgccgt ggcaggcggc ttcctggcct ggtgcgcgtc cgtgatcctg ccggggcttc
     6541 ccgggggatg gcgcatcgtg atcgccatca tcgtggcctc cgccctgggg gcggcgctgg
     6601 gcggcgagga cgcggcatga tctggctcac gatcgccggc atggcgctgg tgacctacgt
     6661 cccgcggttg atcccgatgg ctctgggtcg agacctcccg ctgccaacct ggattcgccg
     6721 ctggctttct ttcttcccct acgcagctct gggcgcgctc atctttcccg ggatcctgac
     6781 ggcaatccag ggcaggccct gggtcagcct ggcggcagga gccaccgcgg cgctggtggc
     6841 tctgagggcc aagaacgcga tcatccccgt gctggcggcc gttgccgttg ccctggcgct
     6901 cggggccgtg gtccgctgag gccggcattc gaccgggcct ccgtccgaca agggcacgag
     6961 cgccgctcgc gagtccttga acccgtggcc gtgtcgccct acattaggtg catggcactg
     7021 ctgggaggct ccaccaagat cttcgtcctg gatacgaacg taatccttca cgatcacgac
     7081 tgcatcaact ccttcgagga gcacaacgtc gtcatcccga tcacggtcct cgaggagatc
     7141 gaccggttca agaagggcaa cgagatcatc aacttcaacg ctcgggagtt ctcccggaag
     7201 ctggactcca tgggaggcga gcagctcctg aacgagggga tccacctcaa gagcggcggg
     7261 atggtgatcg tggagaccaa cgtccgtccg gacgggctcc tcaaggaggt attctgggag
     7321 gacaagcccg atcaccgcat cctctccctg gcgttcaacc tggcacagga gcacggcagg
     7381 gatcgggtga tcctcgtctc caaggatatc aacgcccgga tgaaggccaa ggggatcggg
     7441 cttcgggccg aggactacga gaccggcaag atcggcaaca tcgacgagct gtacaagggc
     7501 aagcagatcc tggaggaagt ggaaggcggg ttgatcgacg agctcttcca ggaaggcgtc
     7561 cttgcccgcg agcgcctctc cctggcccgg gacccctacc ccaatgagta cttcgtcatc
     7621 cgaaacagcc acaagagcat cctggcggtc tacgattccc agcagggcgg attccggcac
     7681 atccagaaga aacgcgccta cgggatcgag cctcgcaacg cggagcagac cttctccctg
     7741 gacgccctgt tcgacgacga catctccctg gtcaccctgg ccggcaaggc gggcacgggg
     7801 aagacgctca tggcgatggc cgcggcgctg gaaaagcgat ccagctacaa gcagatctac
     7861 ctggcgcggc cgatcgtgcc tc
//
LOCUS       DASRZS010000206         6282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2806375, whole genome shotgun sequence.
ACCESSION   DASRZS010000206 DASRZS010000000
VERSION     DASRZS010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2806375"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..957)
                     /locus_tag="VFH83_05195"
     CDS             complement(64..957)
                     /locus_tag="VFH83_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HET6485790.1"
                     /translation="MANAPSRTRNTYHIEEKPFYLPIADEVEVFERAFAEQIPLLLKG
                     PTGCGKTRFVEYMAWSLGQKLFRVAKGAGELNADVKDYRSGVGSLYTVACHEDLTVRD
                     LTGRYIMKGGEAVWVDGPATTAVKEGAILYLDEVVEARKDVTVVIHPLTDYRRILPLE
                     TLGVQIKAPNTFMVVVSYNPGYQSIRKNLKQSTKQRFAALSFSYPPPEKEAVIVEREG
                     GVESAAAEKLVMLANDIRNISTIDLEEGVSTRSIVHAAKLIKRGIPAKRACSIAVAQT
                     ITDEKEEIAAIDVIIDKIFRS"
     gene            1019..1813
                     /locus_tag="VFH83_05200"
     CDS             1019..1813
                     /locus_tag="VFH83_05200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6485791.1"
                     /translation="MGIEVAGAVALVTGAGSGVGEAVAVGLAARGMRVALVGRSTEKL
                     EAARARIQARGGASLVAPCDVGDAAQVSDLKRAVLTGLGCPRVLVNAAGRYGECLPIA
                     DSDPLDWTATLQVNLVGAYLVCNAFLKEMISQGWGRIINVTSAGSLPEPTGIASAYQL
                     SKVGLNHFTRQLARELDGTDVTANAIHPGEVKSPMWEAIRSDAAGRGEPGRGALDWAA
                     WVERTGGDPPEKTLERILELLDPGSEAVSGQFLWIRDGLQKPRPTW"
     gene            complement(1819..2226)
                     /locus_tag="VFH83_05205"
     CDS             complement(1819..2226)
                     /locus_tag="VFH83_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET6485792.1"
                     /translation="MPATISSELVEFIKGKPGWVATASKDGVPNVAIKGSLRVLDEGN
                     LAFADLFSLKTRKNLLENPQVAIMVYEESSRKGYMFKGRAEMLDSGPVFTEMKEQIKQ
                     RMPQLPPPRYVVKVRVDAIFDQSVGPNAGKQIG"
     gene            2468..3022
                     /locus_tag="VFH83_05210"
     CDS             2468..3022
                     /locus_tag="VFH83_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6485793.1"
                     /translation="MNEQIRQIAERIRGLREIARLSVETCAQELGVPVQTYRSYESGE
                     SDIPASFLYQVAGRFHVEMSSLLTGEEPRLRMYSVTRAGRGVKVERRRDYGYQSLAFN
                     FMDKKMEPFLITVEPRPESEPMPTNAHPGQEFNYLLEGVLKVVIEGHEVLLSPGDSLY
                     FDSRHPHGMKALGTTPARFLAVIL"
     gene            3035..4678
                     /locus_tag="VFH83_05215"
     CDS             3035..4678
                     /locus_tag="VFH83_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6485794.1"
                     /translation="MLERFLARQDFTSYGDFREGFRILVPEHFNFAWDVVDSIAKAEP
                     HRPALVWCDEAGASARFSFAQVAEMSIRAATFFLSIGIRRGDPVMLVLKRRYEYWFCL
                     LGLHRIGAVAIPATHLLTRKDIVYRNNAAAVRAVICVNDAKVLDNVDASLPESPTLKH
                     RIALGVKRNGWLDLNAELERADSKLSRQPTENLDTSLLYFTSGTTGMPKMVQHDFAYP
                     LGHILTARYWQNVRDGGLHLTVADTGWAKAAWGKIYGQWLCGTAVFVYDYDRFVPAQM
                     LDVVSKNGVTTFCAPPTIYRFFIKEDFKRFDLSALRYCTVAGEPLNPEVYSRFLDLTG
                     LKLMEGYGQTEMTVAAGTYPWMEPKPGSMGLPSPGYDLDLIDEDGRVCEVGEKGQIVV
                     RTDQRTPVGMFHGYYRDPELTSRAWHDGVYRTGDVAWRDEDGYLWFVGRTDDVIKSSG
                     YRIGPFEVESALIEHAAVLECAVTGVPDPDRGMVVKASVVLAKGYAASPELVTELQEH
                     VKRTTAPYKYPRIIEFVTELPKTISGKIRRVEIREKDVQ"
     gene            complement(4737..>6282)
                     /locus_tag="VFH83_05220"
     CDS             complement(4737..>6282)
                     /locus_tag="VFH83_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET6485795.1"
                     /translation="SIQSLLALRPDSARVRRDGELVEVKPEQVVVGDLIVIRPGERVP
                     LDGTVVSAGGLVDTSALTGESVPRQVRTGSEVLAGFISSDTPLELRVARPVGDSSAAK
                     IIRLVQDAAHRKARTERFISSFARWYTPAVVGAAALVAFLPPLLVPDASLRVWVSRAL
                     TMLVISCPCALVISVPLGYFGGVGGASRRGILVKGAAYLDALAGVRTVVFDKTGTLTR
                     GVFRVREVFPRNGTPKEDLLRYAAVAEAHSNHPIAVSIREAYKGDGLARGDGLGNGGG
                     EYREVSGEGVLARVDGHEVAVGNDRLLHREGVAHDTCRVDGTAVHVTVDGVYAGYLTL
                     GDEIRHGARQAIADLRARGVNRTVLLTGDDPSVASAVAADLGIEESHGGLLPSDKVSH
                     LERIMAQAAPRARTAFVGDGINDAPVLARADVGIAMGGSGSDAAVETADVVLMTDSPS
                     KVVETIDRARATRAIVVQNIAFALGVKGVFLALGAFGIATMWEAVIVDMGVAVVAVLN
                     AARAIR"
BASE COUNT         1016 a         2118 c         2090 g         1058 t
ORIGIN      
        1 tttcgtgccc gtgccggtcg ccgccgtgcc tgtcgccgcc gtggctgcca tgacaccggt
       61 ccctcaggag cggaagatct tgtcgatgat cacgtcgatg gccgcgatct cctccttctc
      121 atcggtaatg gtctgggcca ccgcgatgga gcaggcgcgc ttggcgggga tcccgcgctt
      181 gatgagcttg gccgcgtgga cgatgctgcg ggtgctgacg ccctcctcca ggtcgatcgt
      241 ggagatgttg cggatgtcgt tggccagcat cacgagcttc tccgccgcgg cgctctccac
      301 cccgccctcc cgctccacga tcacggcctc tttctccgga ggcggatagg agaagctgag
      361 ggcggcgaac cgctgtttgg tggactgctt gaggttcttc cggatgctct ggtagccggg
      421 gttgtaggag accaccacca tgaacgtgtt cggcgccttg atctggaccc ccagcgtctc
      481 caacggcagg atcctccggt agtccgtgag cggatggatc accacggtca cgtccttgcg
      541 cgcctccacc acctcgtcca gatagaggat ggccccctcc ttgaccgcgg tggtggccgg
      601 gccgtcgacc cagacggcct ccccgccttt catgatgtag cgtcccgtca ggtcccggac
      661 cgtcaggtcc tcgtggcacg ccaccgtgta gagggagccc acgcccgacc ggtagtcctt
      721 gacgtcggcg ttcagctccc cggctccctt tgccacccgg aacagcttct gccccaggga
      781 ccaggccatg tactccacga accgagtctt cccgcagccc gtgggcccct tcaggagcag
      841 ggggatctgc tccgcgaagg cgcgctcaaa gacctcgacc tcgtcggcga tgggcaggta
      901 gaagggtttc tcttcgatat gatacgtgtt gcgggtacgg cttggggcgt ttgccacgga
      961 ccgacctccg tcttttgcgc gagtgtactc cgggacgggc gcggtagtac agttgcgcat
     1021 gggtatcgag gtggcaggcg ccgtggcgct cgtgacgggc gccggcagcg gcgtcgggga
     1081 agcggttgcg gtgggcctgg ccgcgcgcgg catgcgcgtg gcgctggtgg gccggtccac
     1141 ggagaagctc gaggcggcgc gggcgcgcat ccaggcgcgc ggcggcgcat ccctggtcgc
     1201 cccgtgcgat gtgggcgatg ccgcccaggt gtcggacctg aagcgggcgg tgttgacggg
     1261 tctaggatgc ccccgcgttc tggtcaacgc cgccggccgc tacggcgaat gtctgccgat
     1321 cgccgacagc gaccctctgg actggacggc caccctgcag gtgaacctgg tcggcgccta
     1381 cctcgtctgc aacgcattcc tgaaagagat gatctcccag ggctggggca ggatcatcaa
     1441 cgttacctcg gcgggatccc tgcccgagcc taccgggatt gcctcagcct accagctcag
     1501 caaggtaggg ctcaaccatt tcacgcgcca actggcgcgg gagctggatg gcaccgacgt
     1561 caccgccaac gccatccatc cgggcgaggt aaagagcccc atgtgggaag ccatccgctc
     1621 cgacgcggcc ggtcgcggtg agccgggcag gggcgcgctg gattgggccg cgtgggtaga
     1681 gcgcacgggc ggcgatccgc ccgagaagac cctggagcga atcctggagc tgctggatcc
     1741 gggatccgag gccgtctcag gccagttcct ctggatccgg gacgggcttc agaaaccccg
     1801 tccgacctgg tgaggcccct acccgatctg cttgccggcg ttcggaccca cggactggtc
     1861 gaagatcgcg tccacgcgaa ccttcacgac gtaccggggc ggcgggagct ggggcatgcg
     1921 ctgcttgatc tgctccttca tctccgtgaa gaccgggccg ctgtcgagca tctcggcgcg
     1981 ccccttgaac atgtagccct tccgcgagct ttcctcgtag accatgatcg ccacctgggg
     2041 gttctccagg aggttcttgc gggtcttcag ggagaagagg tccgcgaatg ccaggtttcc
     2101 ctcgtccagg acacggaggg atcccttgat cgccacgttg ggcacaccgt ccttcgaggc
     2161 ggtggccacc cacccgggct tccccttgat gaactccacg agctcggaag agatcgttgc
     2221 aggcatgggc gctccttggt tgagatctga atccagtata gccagcggct tctccgaagg
     2281 gcaacggagg gtcccaggtg gggcgtaacc ttttccctcc cgcgttgttt accttttgat
     2341 tcctcagaga acctccttct gcgagcctcg cgtcgcgata agcggcgcgg ggccgttttt
     2401 ttgggcctat gattccgcgc ccccatgagg taagctgtcg ccacctttgc cccatcggga
     2461 ggctcccatg aacgaacaga tccggcagat cgccgagcgc atccgtggac tccgggagat
     2521 cgcgcgcctc tccgtcgaga cgtgcgccca ggagctgggc gtgcccgtcc agacctaccg
     2581 gtcctacgag agcggggagt cggacatccc ggcgagcttc ctgtaccagg tggcgggccg
     2641 attccacgtg gagatgtcct ccttgctcac cggagaagag ccgcggcttc ggatgtactc
     2701 ggtgacccgg gccggccgcg gcgtcaaggt ggaacggcgt cgagactacg ggtaccagag
     2761 cctggcgttc aacttcatgg acaagaagat ggagccgttc ctgatcaccg tggagccgag
     2821 gccggagtct gagcccatgc cgacgaacgc ccatccgggc caggagttca actacctcct
     2881 ggagggggtc ctcaaggtgg tgatcgaggg gcacgaggtg ctcctttccc ccggtgattc
     2941 cctgtacttc gactcgcgcc atccgcacgg gatgaaagcg ctggggacca cccccgcgcg
     3001 gttcctcgcg gtcatcctgt agaaaggcac tcccatgctc gagcgattcc tggctcgtca
     3061 ggacttcacc tcctacggcg actttcggga gggctttcgg atcctggtgc cggagcattt
     3121 caactttgcc tgggacgtgg tggactcgat tgccaaggcc gagccccatc ggccggcgct
     3181 ggtctggtgt gacgaggccg gcgccagcgc ccggttcagc ttcgcccagg tcgcggagat
     3241 gtccatccgc gccgcgacct tcttcctctc cattgggatc cgacgcgggg acccggtgat
     3301 gctcgtcctg aagcgacgct acgagtactg gttctgtctg ctcggtctgc atcgcatcgg
     3361 ggccgtggcc atcccggcca cacatctgct cacccgaaag gacatcgtct atcggaacaa
     3421 cgcggcggcg gtgagagccg tcatctgcgt gaacgacgcc aaggtcctgg acaacgtgga
     3481 tgcctctcta ccggagtccc ctaccctgaa acaccgcatc gcgcttggcg tcaagaggaa
     3541 cggctggctg gacctgaacg cggagctgga gcgggccgac agcaagcttt cccggcagcc
     3601 gacggagaac ctggacacca gcctcctgta tttcacctcg gggacgacgg gcatgcccaa
     3661 gatggtccag cacgatttcg cgtacccgct gggccacatc ctcacggcca ggtactggca
     3721 gaacgtgcgg gacggcgggc tgcacctgac cgtggcggat accggctggg ccaaggcggc
     3781 gtgggggaag atctacggcc agtggctctg cgggaccgcg gtgttcgtct acgactacga
     3841 ccggttcgtg cccgcccaga tgctggacgt ggtctcgaag aacggggtca ccacgttctg
     3901 cgcgcctccc acgatctacc gattcttcat caaggaagac ttcaagcgtt tcgatctctc
     3961 cgccctccgc tactgcaccg tggcgggcga gcccctcaac ccggaggtct actcccggtt
     4021 cctcgacctc acggggctga agctcatgga aggctacggc cagacggaga tgaccgtcgc
     4081 cgcgggcacc tatccgtgga tggagccgaa gcccggctcc atggggcttc cgtcacccgg
     4141 gtatgacctc gacctgatcg acgaggatgg cagggtctgt gaggtcgggg agaagggcca
     4201 gatcgtcgtc cgcaccgacc agcgaacgcc cgtcgggatg ttccacgggt actacaggga
     4261 ccccgagctg acctcgcggg cgtggcatga cggcgtgtac cggacggggg atgtagcgtg
     4321 gcgggacgag gacggctacc tgtggttcgt cgggcgcacg gatgacgtca tcaagagctc
     4381 ggggtatcgc atcggcccgt tcgaggtgga gagcgccctc atcgagcacg cggcggtcct
     4441 ggagtgcgcg gtcaccggcg tgccggatcc ggatcgcggc atggtggtca aggcttccgt
     4501 ggtcctggcc aagggctacg ccgcgagccc ggagctggtc actgagctgc aggagcacgt
     4561 caagcgcacg accgcgccct acaagtaccc gaggatcatc gagttcgtga ccgagcttcc
     4621 caagacgatc agcggcaaga tccgcagggt ggagatccgc gagaaggacg tgcagtaggc
     4681 ggggccgccc aggtggggcc acccagtcgg ggtcgcctga gaatggtcgc caggggctac
     4741 cggatagcgc gcgccgcgtt caatacggcc acgacggcca cgcccatgtc cacgatcacc
     4801 gcctcccaca tcgtggcaat cccgaaagcg cccagggcca ggaacacgcc cttcacgcca
     4861 agggcaaagg cgatgttctg caccacgatg gcccgggtgg ctcgagcgcg gtcgatggtc
     4921 tccaccacct tggaggggct gtccgtcatc aagaccacat ccgcggtctc caccgcggca
     4981 tccgagccgg agccgcccat ggcgattccc acgtcggcgc gcgccaggac gggcgcatcg
     5041 ttgatcccgt cgccgacgaa ggccgtgcgg gcgcgcggcg ccgcctgcgc catgatccgc
     5101 tccagatggc tcaccttgtc cgagggcagg agcccgccat ggctctcctc gattcccagg
     5161 tcggcggcaa cagccgacgc caccgacgga tcgtccccgg tcagcagcac cgtacgattc
     5221 accccgcgag cccgcaggtc cgctatggcc tggcgcgcgc cgtggcggat ctcgtcccca
     5281 agggtcaggt accccgcgta gacaccgtcg acggtcacgt ggaccgcggt gccatccacc
     5341 cggcaggtgt cgtgcgccac gccttcccga tgcagcagtc ggtcgtttcc gaccgccacc
     5401 tcgtggccgt ccacgcgggc cagcacccct tcgccgctca cttcccgata ctcgccaccc
     5461 ccgttaccca ggccgtcgcc cctggcgagc ccatcgcctt tgtaggcttc ccggatcgag
     5521 acggcaatcg gatggttgga gtgggcctcc gcgacggccg cgtatcgcag aagatcctcc
     5581 ttcggggtgc cgttgcgcgg gaagacctcc ctcacccgaa agacgccgcg cgtgagcgtg
     5641 ccggtcttgt cgaagaccac ggtgcggact cccgcaagcg cgtcgaggta ggcggcgccc
     5701 ttgacgagga tcccccgacg ggacgcgccg cccacgccgc cgaagtagcc aaggggcacg
     5761 ctgatcacca gggcgcacgg gcaggagatg accagcatgg tcagcgctcg ggagacccac
     5821 acgcggaggg aggcgtcggg cacgaggagc ggcggcagga acgccaccag cgccgccgcg
     5881 cccaccaccg ccggggtgta ccaccgggcg aagctgctga tgaaccgctc cgtccgggct
     5941 ttgcggtggg ccgcatcctg gaccaggcgg atgatcttgg cggcgctcga atcccccacg
     6001 ggacgggcaa cgcgcagctc gagcggcgtg tcggagctga tgaagccggc caggacttcg
     6061 gaccccgttc gtacctggcg aggcacggac tctccggtga gcgcggaggt atcgaccagc
     6121 ccgcccgcag agaccaccgt gccgtccagc gggactcgct cgcccggtcg gatcacgatc
     6181 aagtccccca cgaccacctg ctcgggcttc acctccacga gctcgccgtc ccgccgaacc
     6241 cgggctgagt ccgggcgcag agccaggagc gactggatgg ag
//
LOCUS       DASRZS010000207         4798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1268075, whole genome shotgun sequence.
ACCESSION   DASRZS010000207 DASRZS010000000
VERSION     DASRZS010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268075"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VFH83_05225"
     CDS             <1..1071
                     /locus_tag="VFH83_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET6485796.1"
                     /translation="AILRVDMCGVCGTDKHIFRGDASKVRGKSLFPFIEGHEIIGTIV
                     EIGSEAAKVMESSREVLKEGDRVAVAVEVNCGKCWYCKNMYDNITCENQVMAYGVHPG
                     SETSPHLRGGFAEYLYIFPNSTLFKIPDGMSTDAAVFVEEMAVAYSALARATSPYPAI
                     KEGFGPGDSVAILGNGPLGALHGIMAKIQGAGLSIATDLSDTRLDLVKSLYADTTINA
                     SRTPRAERIRMIKEMTGGIGPDLVVESAGEPDVLLEALEMVRKGGTVIEVGNWVDTGQ
                     TVPLNVMQHISSKNLHIHGLYHCGYKWAPVLKVMDEHGDRFPFEKLITHRFTLQQVVE
                     NLSIVTDPNKCMKVEVVPHQKG"
     gene            1102..2265
                     /locus_tag="VFH83_05230"
     CDS             1102..2265
                     /locus_tag="VFH83_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET6485797.1"
                     /translation="MADQSIGVAEYQERFRSVQAVLKERGIDVLVTFGSESEPANLLY
                     LANYWPAFETGAVVVPQEGRPALVIGPEARTFAEDSRVIPDLFQVLEHRESSEPDYPD
                     KKLATYEEVFQFTLGGRKPRRVGISGMAFISVPVHQAIVRACGGAEIVKADEVLIRLR
                     MKKSANEIRLLRESAAITLKAFEAGMKNLRPGMEEVEVAGFITAEMYRNRAESFAYSP
                     FVLSGARTNQAISRASHRVIGKNEPIQFSLGCKYYGYASSIGRPLCVGRMPARYRELV
                     EVGIKAQDLVIASLKPGIPAKEVYGKYWDYLTRAGYASFFLYGPCHGTGIMECEHPFL
                     EANSGYLLEEGMTFQVDVFLGNPEFGLRFEDGAVITRSGAEPFTSAYRSVIEL"
     gene            2274..2657
                     /locus_tag="VFH83_05235"
     CDS             2274..2657
                     /locus_tag="VFH83_05235"
                     /inference="COORDINATES: protein motif:HMM:NF018767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HET6485798.1"
                     /translation="MKRTGGSRTWYVADGWMPLQKPTEKAPGYEGHEAIMILNTSPRD
                     AYVSMDVYFEEKDPVEGIQLRVPAKRIKCFRMDRPGDIGGLKLDRLYQYALRFRSNVP
                     VVVQYGRMDVTQTNLAYIGTMGHAE"
     gene            2647..3849
                     /locus_tag="VFH83_05240"
     CDS             2647..3849
                     /locus_tag="VFH83_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET6485799.1"
                     /translation="MPSDARSARVHLAVDNCFASKRWTRPYQWMELVRDLGLRCVEAS
                     ADTEADPLYCGEDYMARWAEEVSRSGDATGVRVVNLYSGHGTYTTLGLGHTDERVRRR
                     MLELWIKPMVRTAASLRAGLGFYCHAFDDEVLQDPGTYAAKMDELNGELAEVAAFAAA
                     EGVTVGVEQMYSPHQYPWTIEGARDLILRVSQAGGHPLYITIDTGHQTGQRRFRRPDA
                     STIVERASSGPAASASAPWLGSRDAQARFERCAADGRGVSSGDLAFLAEDMDRHPFLF
                     SSPRDSDPYAWIEDLGRFSPIVHLQQVTGASSSHLPFTEANNRSGAIQPDKVLASLRA
                     SCESPVPPGFPPPPEDLYLTLEIFAGTAETPSEILAKLRESARFWRRYVPEDGLPLAE
                     LAGVPGRP"
     gene            3905..>4798
                     /locus_tag="VFH83_05245"
     CDS             3905..>4798
                     /locus_tag="VFH83_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6485800.1"
                     /translation="MAEMNLKTIARIAKVDHSTVSRALRGSPVVKQSTRERILEIARA
                     YHYVPNEIARSLKTKKTKIVGLIVSDITNPFFTEIINATEAYLARNSYNIILCNTNYS
                     VAKEEKFLHILFSRSVDGIILSPTSLEHLHTEFFGRYRIPSVLLDVKVRNLGTNSVYV
                     DQELGAFTAVRYLIEKGHKRIAFLAGPETLSSSQQAIKGYLKAHRKAEVPIDEELILR
                     IPQDYDAAYAATQKLLKRSRKITAIFSLSDFLCIGVYRALQEANLRIPDDIAVLGYDD
                     LSLMRFLRPALTTVRQPNADIG"
BASE COUNT          870 a         1550 c         1571 g          807 t
ORIGIN      
        1 gccatcctcc gggtggacat gtgcggggtc tgcggcaccg acaaacatat cttccggggg
       61 gatgccagca aggtgcgcgg caagtccctg tttccgttca tcgagggtca cgagatcatc
      121 gggaccatcg tcgagatcgg cagcgaagcg gccaaggtga tggagtcctc ccgagaggtc
      181 ctgaaagagg gcgatcgggt ggcggtggcc gtggaggtga actgcgggaa gtgctggtat
      241 tgcaagaata tgtacgacaa catcacctgt gagaaccagg tgatggcgta cggcgtccat
      301 cccggctcgg agacatcgcc gcacctgcgc ggcggcttcg ctgagtacct gtacatcttt
      361 ccgaactcca cgcttttcaa gattccggac ggaatgtcca cggacgccgc cgtctttgtg
      421 gaggaaatgg cggtggccta cagcgcgctg gctcgcgcca cctctcctta ccctgctata
      481 aaggaaggat tcggtcccgg agactcggtg gccatcctgg gcaacggccc gttgggcgcc
      541 ctccacggga tcatggccaa gatccagggg gcgggactct ccatcgccac ggacctttcc
      601 gacacgcggc tggacctggt gaaaagcctg tacgcggaca ccaccatcaa cgcttcccga
      661 accccgaggg cggagcggat ccgaatgatc aaggagatga ccggcgggat cgggccggac
      721 ctcgtcgtcg agtccgcggg agagccggac gtgctcctgg aggccctgga gatggtccgc
      781 aagggcggca cggtgatcga ggtgggcaac tgggtggata ccggccagac ggttcctctc
      841 aacgtgatgc agcacatctc ttccaagaac ctccacatcc acggcctgta tcactgcggg
      901 tacaagtggg ctccggtcct gaaggtcatg gatgagcacg gagatcgctt ccccttcgag
      961 aagttgatca cccaccggtt cacgctccag caggtcgtgg agaacctctc catcgtcacg
     1021 gaccccaaca agtgcatgaa ggtggaagtg gtcccccacc agaaggggta gccggctccg
     1081 gcggcacgaa aggaacagag catggcggac cagtccatcg gagtagcgga gtaccaggag
     1141 cgattccgca gcgttcaggc tgtcctgaag gagcgcggga tcgacgtcct ggtgacgttc
     1201 ggctcggagt ccgagccggc caacctgctg tacctggcca actactggcc ggcgttcgag
     1261 accggcgccg tggtggttcc ccaggaggga cgcccggccc tggtcatcgg accggaggcc
     1321 cgaaccttcg ccgaggacag ccgggtcatc ccggatctgt tccaggtcct ggagcatcgc
     1381 gagtcctccg agccggacta cccggacaag aagctcgcca cctatgagga ggtcttccag
     1441 ttcaccctcg gcgggcgcaa gccccggagg gtcggcatct ccgggatggc cttcatcagc
     1501 gtgccggtgc accaggccat cgtgcgcgcc tgcggcgggg cggagatcgt caaggcggac
     1561 gaggtcctga tccggcttcg gatgaagaag agcgccaacg agatccggct ccttcgagag
     1621 tcggccgcca tcacgctcaa ggcgttcgag gccgggatga agaaccttcg ccccggcatg
     1681 gaagaggtgg aggtggcggg cttcatcacg gcggagatgt accgcaaccg ggcggagagc
     1741 ttcgcctact ctccgttcgt tctctctgga gcgcggacca atcaggccat cagtcgcgcc
     1801 tcccaccggg tcatcgggaa gaacgagccc atccagttca gcctcgggtg caagtactac
     1861 ggctacgcgt cctccatcgg ccggcccctc tgcgtgggcc gcatgcccgc tcggtaccgc
     1921 gagctggtgg aggtcgggat caaggcccag gacctggtga tcgcctctct caaaccgggg
     1981 attcccgcca aagaggtcta tggcaagtac tgggactatc tgacccgcgc cgggtacgcg
     2041 agcttcttcc tctacggtcc ctgccacggc accgggatca tggagtgcga gcatccgttc
     2101 ctggaggcca atagcgggta cctcctggag gaaggcatga cgttccaggt ggacgtcttc
     2161 ctggggaatc ccgagttcgg gctccggttc gaggatgggg ccgtcatcac gcggagcggc
     2221 gcggagccgt tcaccagcgc gtatcgctcg gtgatcgagc tgtaggggga gccatgaagc
     2281 gcaccggtgg atccaggacc tggtacgtcg cggatggctg gatgccgctc cagaagccaa
     2341 ccgagaaggc accggggtac gagggacacg aggccatcat gatcctcaac acaagccctc
     2401 gggacgccta cgtctcgatg gacgtgtact tcgaggaaaa ggatcccgtg gaggggatcc
     2461 agctccgggt cccggcgaag cggatcaagt gcttccgcat ggaccgcccg ggtgacatcg
     2521 gcggcctgaa gctggaccgg ctctaccagt acgcccttcg gttccgctcc aacgtcccgg
     2581 tcgtggtgca gtacggccgc atggacgtca cccagaccaa cctcgcctac atcgggacca
     2641 tgggccatgc cgagtgacgc gcggtctgcg cgcgtccacc tggccgtaga caactgcttc
     2701 gcctccaagc gctggaccag gccgtaccag tggatggagc tggtccggga cctcgggttg
     2761 cggtgcgtgg aggccagcgc ggacacggag gcggaccccc tctactgcgg tgaggactac
     2821 atggctcgct gggcggagga ggtgtctcga agcggtgatg ccaccggcgt ccgggtggtg
     2881 aacctctact cagggcacgg gacctacacg accctggggc tcgggcacac cgacgagcgt
     2941 gtgcgtcgcc ggatgctgga gctgtggatc aagccgatgg ttcggaccgc ggccagcctg
     3001 cgggcggggc tcgggttcta ctgccacgcg ttcgacgacg aggtcctgca ggacccgggt
     3061 acctacgcgg ccaagatgga cgaactgaac ggcgagctgg cggaggtcgc cgccttcgcc
     3121 gcggcggagg gtgtcacggt cggcgttgag cagatgtaca gtccgcacca gtacccgtgg
     3181 accatcgagg gagcgcggga tctgatcctg cgggtcagcc aggccggcgg ccatcccctg
     3241 tacatcacca tcgacacggg tcaccagacg gggcagcgca ggttccgtcg gcccgacgcc
     3301 tcgaccatcg tcgaacgggc gagctctggt cccgctgcgt cggcgtcggc accgtggctg
     3361 gggtcccgcg atgctcaggc gcgtttcgag aggtgcgcgg cggacggtcg gggcgtctcc
     3421 agcggggacc tggccttcct ggcggaggac atggaccgtc acccgttcct cttctcttct
     3481 ccgcgggact cggatcccta cgcctggatc gaggacctgg gtcggttctc tccgattgtg
     3541 cacctccagc aggtgacggg cgcctcctcc tctcacctcc cgttcaccga ggccaacaat
     3601 cgaagtggcg ctatccagcc ggacaaggtc ctggcctccc ttcgcgcatc gtgcgagagt
     3661 ccggtgccgc cgggttttcc gccccctccc gaggatttgt acctgaccct ggagatcttc
     3721 gccggcacgg cggagacccc ttcggagatc ctcgccaagc tgcgggagag cgcgcggttc
     3781 tggcgccggt acgtgccgga ggacggcctt ccgctggcgg agttggccgg cgttcccggt
     3841 cggccttgac cggcccattg agccggtgta gtctgtggaa aacgcgccct agaacagggg
     3901 actcatggcg gagatgaacc tcaagaccat agcgcgaatc gcgaaggtcg atcattcgac
     3961 ggtatcccgc gccctgcgcg gcagccccgt ggtgaagcag tccacccggg agcggatcct
     4021 ggagatcgcc cgcgcctacc actacgtgcc caacgagatc gcgcgctccc tgaagacgaa
     4081 gaagaccaag atcgtggggc tgatcgtctc ggacatcacg aacccgtttt ttacggagat
     4141 catcaacgcc accgaggcgt acctggcccg caacagctac aacatcatcc tctgcaacac
     4201 gaactacagc gtagcgaagg aggagaagtt cctgcacatc ctcttctcgc ggagcgtgga
     4261 cggcatcatc ctgtccccca cgtccctgga gcacctgcac accgagttct tcggccgcta
     4321 ccggatcccc agcgtccttc tggacgtcaa ggtccgcaac ctcggcacca acagcgtcta
     4381 cgtggaccag gagctggggg cattcacggc ggtccgatac ctcatcgaga aggggcacaa
     4441 gcgcatcgcg ttcctcgccg gacccgagac cctctcctcc tcccagcagg ccatcaaggg
     4501 ctacctcaag gcccatcgca aggcggaggt gcccatcgac gaggagctga tcctgaggat
     4561 cccgcaggac tacgacgcgg cctacgccgc gacgcagaag cttctcaagc gcagccgtaa
     4621 gatcacggcg atcttctctc tcagtgattt tctctgcatc ggggtctacc gcgcgctcca
     4681 ggaagccaac cttcggatcc cggacgacat cgcggtcctg ggctacgacg acctgagcct
     4741 catgcggttc ctgaggcccg ctctcacgac ggtccgtcag cccaacgcgg atatcggc
//
LOCUS       DASRZS010000208         6353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4349113, whole genome shotgun sequence.
ACCESSION   DASRZS010000208 DASRZS010000000
VERSION     DASRZS010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4349113"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VFH83_05250"
     CDS             complement(<1..993)
                     /locus_tag="VFH83_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6485801.1"
                     /translation="MPATATRLGYWIDNGIRASNTAKYMDCFDPSTGEVIAQAPQCTA
                     QEVDAAVASAAAAFPEWAATPASKRVQVLFRMKALLDQHLDELTRMVAMENGKVWDEA
                     MGDVLRVTEVVEFACGIPQLLQGPALMNVTAGHDTTQYREPVGVFAGIAPWNFPAMIP
                     MGWMAPLCIATGNTMVIKAASFVPQSSLRIVELWKEAGLPKGVLNVVTCSRNEAEILL
                     KHPDVKGVTFVGSTAVGKHIYATAAANGKRVQALTEAKNHALVLRDAALERSAAGVVN
                     SACGCAGERCMALPVVVVEEAIADSFVDALARRMKALKIGPAYDKTSQLGPVVND"
     gene            1096..1860
                     /locus_tag="VFH83_05255"
     CDS             1096..1860
                     /locus_tag="VFH83_05255"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6485802.1"
                     /translation="MESFRPVRKQKASDLIVEEIWKMILGGKLKPGDRLPPEHRLVER
                     FQVSKVTLREALQTLETYGHITRKRGPGGGSIVLDIAPTKGIGLLANYLSLSNLSIDE
                     LMEARLRIDPMVAGLAASRITQDGIERLQRLLCEHEEDFRLHGGSKRGWEFYILLAEL
                     TGNRALRVIEELLIRLFMDAEFSLSIGDIGMTPEETAYNAAVLPVNRRIADAVVAGDP
                     DRASREMVEAIRGFSSRIAEYAAHSLRKPGAVTLPQ"
     gene            complement(1883..2809)
                     /locus_tag="VFH83_05260"
     CDS             complement(1883..2809)
                     /locus_tag="VFH83_05260"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET6485803.1"
                     /translation="MNLEVFVLGTGGMMPLPGRGLTSVLLRREGDLFLFDCGEGTQIG
                     LRRLNLRWKKITAIFISHTHADHVTGLPGILMLSSQVDREEPLYIYGPPKIREFVDAM
                     RRTLEMYINYEIVVREVEGGSTIVQGEGFAIRSHSLSHTKPCLGYCLEEAERPGVFHP
                     ETALELGVPRGPLWSRLQDGETVSLPNGTSVQPCQVLGKPRKGRKFCFATDTIAAHGL
                     AEFARGSDLFICEGMFADDLADTAAEKKHLTASQAARIAKEGGVKQLGLIHYSPRYSE
                     RELKKLLQEARDIFPDSFLTKDLQHIELPFEE"
     gene            complement(2824..5823)
                     /locus_tag="VFH83_05265"
     CDS             complement(2824..5823)
                     /locus_tag="VFH83_05265"
                     /inference="COORDINATES: protein motif:HMM:NF019966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET6485804.1"
                     /translation="MDARQDRSFEVLSQRPLPEYRADATHLRHTATGCEILHLKTLDA
                     ENLFAFCFTTPPRDDTGVSHIIEHSVLSGSRRFPLKEPFSALMKGSMNTFLNALTYPD
                     RTVYPAASCNRADFFNLLSVYGDAVFSPLLRKETFMQEAWRLEEAEEGGPNESASDGL
                     RYAGVVYNEMKGAYSSPDSIVGEWAIRSLFPDTPYQFDSGGDPRFIPSLTLESARQFH
                     ARYYHPSNCRIFLYGNIPLSDILARLQDEFLSGFTAQSIDARIALQRRWPSPRRLEKT
                     FPVKPQTPLEGRSIVTMTWLLPPVTDASALVLHEVLSEILIGSAGSPLRKRLVDSGLG
                     EDLSPVSGLETDLQEMIFAAGLRATEPDREDRIVALILETLTSLASQGLDPRLVQSMI
                     NRVEFRHREIRGGGGPYALRLMGRALRGWIHGMDPIASLEFVGAMTQLKARLASDHRL
                     LEDRILADLVRNSHRLTLVVRPDPEQGEREASEEGDRVRTAVSGLSPEEKGNLREEAD
                     RFRAYQLAPDTPEALALLPTLKRSDIPTAIERIPSEETRTRSGIPLSLHDIFTNEIIY
                     LDLCFPTDSLSDEDALLLPLLGKAITGGGLPDMSYDQVALELYRLTGGFSSSLDSGGV
                     VGRPGAGVEFAFFRMRCLLDNLPAAAALTGRLLFSADFRDKARLRDLLLEMRNDLKSA
                     LVPGGHQFAMLRAASRISRTVAREEQWRGVTQLLYLEEIAKDIDGRLPELSERLESVR
                     SRLLSRAVLRVNATADRGAFKAIAEAAEGIAAALPSAQSPGGAGAAKRPAGGAPSYDG
                     GAAPRGESLLSSAAVGYVATAVPGYFYDDPFNGPVAVLGHLLSTGYLWEKVRMEGGAY
                     GAFSFPRNTDGLFLFGSYRDPKIVSTVRAFREGLARLVQGGVTEAEVDQGVIGTIGRE
                     DRPMDPGEKGFVSMQRSLHGIRDDLRQTRRDRLLATSLKDLSEAAQGLLAGFDRSFTA
                     VIANRQSLDGAAEELPELRSAVVDLPE"
     gene            5880..>6353
                     /locus_tag="VFH83_05270"
     CDS             5880..>6353
                     /locus_tag="VFH83_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET6485805.1"
                     /translation="MSDPLRHRLQRHALFRRSIREFFAERGYVEVDTPSLAPFLIPEP
                     AIDVFATRLHRRRGSETDLWLAPSPELWMKRLLGAGSGNIFQLARAFRNGDLDSPIHN
                     PEFTLLEWYTMDSGYEEAIGITEDLFRHLLDRGVGGPRAERLRPPFHLLRMSEAFQ"
BASE COUNT         1104 a         2091 c         2077 g         1081 t
ORIGIN      
        1 gtcgttcacc acgggcccga gctggctcgt cttgtcatag gccggcccga tcttgagagc
       61 cttcatgcgg cgcgccagag cgtccacgaa agaatccgcg atggcctctt ccaccacgac
      121 gaccggcagc gccatgcagc gttccccggc gcagccgcac gcggagttca ccactccggc
      181 ggcggaccgc tccagcgccg cgtccctcag gaccagcgcg tggttcttgg cctcggtcag
      241 agcctggacc cgcttgccgt ttgccgcggc ggtcgcgtag atgtgcttgc cgacagcggt
      301 cgagccaacg aacgtcaccc cttttacgtc ggggtgcttc agcaggattt ccgcctcgtt
      361 gcgggagcag gtgacgacgt tgagcacacc cttggggagg cctgcctcct tccagagctc
      421 cacgatccga agcgagctct gaggcacgaa gctggcggcc ttgatcacca tggtgttccc
      481 cgtggcgatg cagagagggg ccatccagcc catggggatc atcgcgggga agttccacgg
      541 cgcgatgccg gcaaacactc cgacgggctc gcggtactgc gtagtgtcgt gccccgcggt
      601 cacgttcatg agcgcggggc cctgcagaag ctgcgggatc ccgcaggcga actccaccac
      661 ctccgtgacc cgcaggacgt cccccatcgc ctcgtcccag acctttccgt tctccatggc
      721 aaccatgcgc gtcagctcgt ccaggtgctg gtccaggaga gccttcatcc ggaacaggac
      781 ctggacgcgt ttgctggccg gcgttgcggc ccattccggg aatgcggccg cagcggatgc
      841 cacggcggcg tcgacctcct gggcggtgca ttggggtgcc tgggcgatca cctctcccgt
      901 ggaggggtcg aaacaatcca tatatttcgc tgtattcgat gcgcggatac cgttgtcgat
      961 ccagtagccg agcctggtgg cagttgctgg catggaatcc tcccgatgcg ctcgggcaag
     1021 cgcctccgtc tgcccgattg agctaatcat tagtttttta tactataggg gacggagagt
     1081 caaggagacc atcccgtgga gagtttcagg ccggtccgca agcagaaggc gtctgacctc
     1141 atcgtagagg agatctggaa aatgatcctc ggggggaagc tcaagccagg agacaggctt
     1201 ccgccggagc accggctggt ggagcggttc caggtgagca aggtgacgct tcgagaggcc
     1261 ctccagacgc tcgagaccta cggacacatc actcgaaaac gcgggcctgg cggcgggagc
     1321 atcgtcctcg acatcgcgcc caccaagggg ataggcctcc ttgccaacta cctcagcctc
     1381 tccaatctct ccatcgacga gttgatggag gcgcgcctgc ggattgaccc gatggtcgcc
     1441 gggctggccg cctcccgcat aacgcaggac ggaattgagc ggctccagag gctcttgtgc
     1501 gagcacgaag aggacttccg gctgcacggc ggctccaaac gcggctggga gttctacatc
     1561 ctccttgccg agctcacggg caaccgggcg ctgagggtga ttgaagagct tctgatccgg
     1621 ctcttcatgg atgccgagtt ctccctttcg attggcgata tcgggatgac tccggaggag
     1681 acggcctaca atgccgccgt gctccccgtg aaccgccgta ttgcggacgc ggtggtcgcg
     1741 ggggatccgg accgcgccag ccgcgagatg gtggaagcca tccggggctt ttcgagcagg
     1801 atcgcggagt acgcggcgca tagcttgcgg aaacctggag ccgtcaccct tccccagtag
     1861 gagcccgccg cccgggagat cgctactctt cgaaaggcaa ctcgatgtgc tggaggtcct
     1921 tggtcaggaa ggagtccggg aagatgtccc gcgcctcctg gaggagcttc ttgagctctc
     1981 gctcggagta ccgggggctg tagtggatca gaccgagctg tttcacgccc ccctccttgg
     2041 cgatcctggc tgcctgggag gcggtcaggt gcttcttctc cgcggccgtg tccgccagat
     2101 cgtccgcaaa catcccttcg cagatgaaaa ggtccgatcc gcgggcgaac tccgccagcc
     2161 cgtgggcggc aatcgtatcc gtggcaaagc agaacttccg tcccttgcgg ggcttcccca
     2221 acacctggca cggctggacg ctggttccgt tcggcaggct caccgtctca ccgtcctgca
     2281 gccgggacca caggggacct cgcggcacgc cgagctccag cgccgtctct gggtggaaga
     2341 cccccggccg ctccgcctct tccaggcagt atcccagaca cggcttggtg tgcgacagcg
     2401 aatggctccg gatggcgaag ccctctccct gcacgatggt cgagccgccc tccacctcgc
     2461 gcaccacgat ctcgtagttg atgtacatct cgagcgttcg gcgcatcgcg tccacgaact
     2521 cgcggatctt cggcggcccg tagatgtaca ggggctcctc ccgatccacc tgcgaggaca
     2581 gcatgaggat ccccgggaga ccggtgacat ggtccgcatg ggtgtgagag atgaagatgg
     2641 cagtgatctt tttccagcgg aggttgaggc gccgcagccc gatctgcgtg ccctccccgc
     2701 aatcgaacag gaagagatcc ccttccctgc ggaggagcac cgaggtcagc cctctccccg
     2761 gcaagggcat cattccaccg gtgcccagca cgaaaacttc gaggttcatc ctgcagccgc
     2821 tctctattcc gggaggtcga ccacggccga gcggagctcc ggcagctcct ccgccgctcc
     2881 gtccagcgac tggcgattgg cgatcaccgc cgtgaagctt cggtcgaacc ccgccaggag
     2941 cccctgggca gcctccgaaa gatccttcag gctcgtggcc aggagccggt cccgtcgcgt
     3001 ctggcggaga tcatcccgta tcccgtggag ggagcgctgc atgctcacga accccttttc
     3061 cccggggtcc atgggccggt cctctcgccc aatggtgccg atgacgccct ggtccacctc
     3121 tgcctcggtg acgccaccct ggacgagtct cgccaggcct tctcgaaacg cccggaccgt
     3181 cgagacgatc ttggggtcac ggtaggagcc gaacaggaac agaccgtcgg tgttcctggg
     3241 gaaggagaac gcgccgtagg ctcccccttc catgcgcacc ttctcccaga ggtagcccgt
     3301 cgagaggagg tggccaagaa ccgccacggg tccattgaac ggatcgtcgt agaagtaccc
     3361 cggcacggcc gtggcaacgt atccgaccgc cgcgctggac aggagggact ccccacgcgg
     3421 cgcggcgccg ccgtcatagg agggcgctcc accggccggc cgcttcgccg cgccggcgcc
     3481 tcccggcgat tgcgcggaag gcagcgcagc cgcgatgccc tccgccgcct cggcaattgc
     3541 cttgaacgcg ccgcggtcag ccgtggcgtt cacccgcagc accgcgcggc tcagaaggcg
     3601 ggagcgcacg gactcgagcc tctcggagag ttccgggagc cttccgtcga tgtctttggc
     3661 gatctcctcg aggtagagaa gctgcgtcac tccacgccac tgctcttccc gagcgacggt
     3721 ccgggagatc ctgctggccg cgcgcagcat ggcgaactgg tggccgcccg ggaccagggc
     3781 ggacttcagg tcgttgcgca tctccaggag caggtcacgc aaccgggcct tgtcccggaa
     3841 atcggcggag aacaggagtc ggcccgtcag ggcagcggcg gcaggcaggt tgtccagcag
     3901 gcaccgcatg cggaagaagg cgaactcaac gcccgcgcca ggcctgccga ccacgccacc
     3961 ggagtccagg gaagaggaga agccgcccgt caggcggtac agctcgaggg ccacctggtc
     4021 gtagctcatg tccgggagcc caccgccggt gattgccttg cccaggagcg gcagcagcag
     4081 ggcatcctcg tccgaaaggc tgtccgtcgg gaagcacaga tccaggtaga tgatctcgtt
     4141 tgtaaagatg tcgtgaaggg acaacgggat gccggagcgg gtcctggttt cctccgaggg
     4201 gatcctctcg attgccgtcg ggatgtcgga tcgcttcagg gtcggcagaa gcgcgagagc
     4261 ctccggggtg tccggcgcga gctggtacgc gcggaacctg tcggcctcct cccggagatt
     4321 ccccttctcc tccggggaga gcccggacac cgcggtacga acgcgatccc cttcctcgct
     4381 ggcctcccgc tctccctgct cggggtcggg ccgcaccacg agcgtcaacc ggtggctgtt
     4441 ccgcacgagg tccgcaagga ttcggtcctc cagaaggcgg tggtccgagg ccagacgcgc
     4501 cttgagctgg gtcatggcgc ccacgaactc cagcgacgcg atgggatcca tcccatggat
     4561 ccagccccga agcgcgcgcc ccatgagacg cagcgcgtag gggccgcccc cgccgcggat
     4621 ctcgcggtgg cggaactcga cccggttgat catcgactgg accagcctcg gatcgaggcc
     4681 ctgggaggcc agggaggtca gggtctccag gatcagcgcg acaatacggt cctcacggtc
     4741 cggctcggtc gcgcgaaggc cagcggcgaa gatcatctcc tggagatccg tctccagccc
     4801 gctgaccggc gaaaggtcct ctcccagtcc ggaatccacc aggcgcttgc gaagcggaga
     4861 cccggcgctg ccgatcagga tctctgaaag cacctcatgc aggaccaggg cggaggcatc
     4921 cgtcacgggc ggcaggagcc aggtcatggt gacgatggac cggccctcca gtggggtctg
     4981 cggctttacc gggaacgtct tctccagccg gcggggagaa ggccaccgcc gctgaagagc
     5041 gatcctggca tcgatgctct gggccgtgaa gccggagagg aactcatcct gaagcctggc
     5101 caggatgtcg gagaggggga tgttcccata gaggaagatc ctgcagttcg acgggtggta
     5161 gtaccgagcg tgaaactggc gggccgattc gagggtgagg gacgggatga acctggggtc
     5221 cccgccggag tcgaactggt agggggtgtc cggaaacaga gaacgaatgg cccactcgcc
     5281 gacaatggaa tcggggctcg agtaggcgcc cttcatctcg ttgtagacga caccggcgta
     5341 ccgcaggccg tctgatgccg actcgtttgg accgccctct tccgcctcct ccaggcgcca
     5401 cgcttcctgc atgaacgtct ctttccgcag gagcgggctg aacaccgcgt ccccgtacac
     5461 ggacaagagg ttgaagaagt ccgcccgatt gcagctagcg gcgggataca ccgtacggtc
     5521 gggataggtc agcgcgttca ggaacgtgtt catgctgccc ttcatgaggg cggagaacgg
     5581 ctccttcagc ggaaagcgac gggaaccgga cagtaccgag tgctcgatga tgtgggacac
     5641 gccggtgtcg tcccgcggcg gcgtcgtgaa gcagaacgcg aagaggttct ccgcgtccaa
     5701 ggtcttcaga tggaggatct cgcacccggt cgccgtgtgg cgcaggtgcg tggcatccgc
     5761 ccggtactcc ggcagcggac gctgggaaag aacctcgaag ctgcgatcct ggcgagcatc
     5821 catggcgcta aagaatatac ggttcgggtc tcggcgtgtc tatgttatac tgctccggca
     5881 tgagcgatcc gctccgacac aggcttcagc gccatgccct ctttcgccgt tccatccgtg
     5941 agttcttcgc cgaacggggc tatgtcgagg tggacacgcc tagcctggcg cctttcctca
     6001 tccccgaacc ggcgatcgac gtcttcgcca cccggctgca tcgacgccgc ggctccgaaa
     6061 cggacctgtg gctcgcccct tcccccgagc tgtggatgaa gcggctcctg ggcgcgggca
     6121 gcggaaacat cttccagctg gcccgggcct ttcgcaacgg ggacctggac agtccgatcc
     6181 acaacccgga gttcacgctc ctggagtggt acacgatgga cagcggttac gaagaggcca
     6241 tcggcatcac cgaggatctc ttccgtcacc tcctggaccg aggcgtcgga gggccgcgcg
     6301 cggaaaggct gcggccgccg ttccatctac tccggatgag cgaggcgttt cag
//
LOCUS       DASRZS010000209         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5506777, whole genome shotgun sequence.
ACCESSION   DASRZS010000209 DASRZS010000000
VERSION     DASRZS010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5506777"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="VFH83_05275"
     CDS             complement(<1..1048)
                     /locus_tag="VFH83_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6485806.1"
                     /translation="MNGRFVLFLATRTLRGRSGTARYLRGSVLGIALSLVPLIVVMEV
                     STGMIDGITARLLEVGTYHLQVPMLPDSSGATLTETAQRVAAVPGVSLAVPERQGNAL
                     LVSTHGAAGVTVRFVPPDLPATDPGFRSYVTLDSGAASLSDPRGILLSKALAQTLGVG
                     TGQEVSLLTTWGQSLSGPPRLTQVHVSGVYETGYQELDKTLAYASLALADSVLSPRAA
                     SAFVGVKVADPFADLSAISRAVSAAAGHGARAVSWREIEYSRLASFRTTKALLLFIMG
                     LIVLVASVNVSSSVLMIVFERRHDLGILKSVGAGPRSLALSFLLSGFATGLIGTALGI
                     GLGLLVAVNVNEVIS"
     gene            complement(1045..1731)
                     /locus_tag="VFH83_05280"
     CDS             complement(1045..1731)
                     /locus_tag="VFH83_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485807.1"
                     /translation="MSDPLPLVSVTSLARHFDNGLERLEVLKGVNLDVMPGSTVAVSG
                     ESGCGKSTLLGLIGGLDRPSSGRILVDGREIGSLDEIQLSRYRNRDVGFIFQFHFLLR
                     DFTALENVIMPGMLGTLSTRRLRERGRSLLAEVGLESRMHAWPLELSGGERQRVAVAR
                     ALVNEPRLILADEPTGNLDERNARTVEEMLFALVERHARTMMLVTHDVHLARRAQRCF
                     RLSHGELTPV"
     gene            complement(1724..>2254)
                     /locus_tag="VFH83_05285"
     CDS             complement(1724..>2254)
                     /locus_tag="VFH83_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6485808.1"
                     /translation="KSFFDALFVEKLMMMVLVGLIFVVVGFNVYHSLRRSVHERMEEI
                     AVLKAVGVPPQRIQGVFILQGLFIGLAGGVIGLIVGLALAVNVNGVFDVVQGIVNGVL
                     GMARLLAMPFLALGGEDFAIFSPAYFYLSSVPSHVYPTEAFLVVFFAVLSCAGAALGA
                     SRAVSRFRPAEVLRYE"
BASE COUNT          440 a          763 c          754 g          297 t
ORIGIN      
        1 ccgagatcac ttcgttgacg ttcacggcca ccaggagacc cagcccaatg cctagagcgg
       61 ttccgatcag tccggtggcg aagccggaaa ggaggaacga gagcgccagg gagcgcggcc
      121 cggcacccac gctcttcagg atgcccaggt cgtgccgccg ctcgaatacg atcatgagga
      181 cggaagagga gacgttcacg gaagctacca ggacgatcag gcccatgatg aaaagcagga
      241 gagccttggt ggtccgaaag ctcgccaggc gcgagtactc gatctctcgc cagctcacgg
      301 cccgggcgcc gtgtcccgcc gcggcggaaa cggcgcggga gatcgccgag agatcggcga
      361 acggatcggc gactttgacg ccgacgaaag cgctggccgc ccttggagag agcacggaat
      421 ccgccagggc cagagacgca taggccagcg tcttgtccag ctcctggtag cccgtctcgt
      481 agacgccaga aacgtggacc tgggtgagcc ggggcggccc actcagggac tggccccagg
      541 tcgtgaggag gctcacctcc tggcccgttc cgaccccaag ggtctgggcc agcgccttgc
      601 tgaggaggat gccgcgcggg tctgagaggg acgccgcgcc agagtccagg gtgacgtagg
      661 accggaaccc cgggtcggtt gcgggcagat ccggcggcac aaagcgcacc gtgaccccgg
      721 ccgcgccatg cgttgagacc aggagggcgt tgccctggcg ctccggcacc gccaagctca
      781 ctccgggcac cgctgccacg cgctgggccg tctccgtgag cgtcgccccg gaggaatccg
      841 gcagcatggg cacctggaga tggtacgtac ccacctccag caggcgtgcc gtgatgccat
      901 cgatcatccc cgtcgagact tccatcacga cgatgagcgg caccaggctg agagcgattc
      961 ccaggaccga gccgcgaagg tatctcgccg tcccgcttct gccccgaagc gttcgcgtgg
     1021 cgagaaagag cacgaaccgg ccgttcatac tggtgtgagc tccccgtggc tcagccgaaa
     1081 gcagcgctgg gcccggcgag ccaggtggac gtcatgggtc accagcatca tggtccgggc
     1141 gtggcgctcc acgagcgcga agagcatctc ctcgacggtc cgggcgttgc gctcgtccag
     1201 gttcccggtc ggctcgtcgg ccaggatgag ccgcggctcg ttcaccaggg cgcgagccac
     1261 cgctacccgc tgccgctcac cgccggaaag ctccagcggc caggcatgca tccggctctc
     1321 cagcccgacc tccgccagaa gcgaccggcc gcgctcgcgg agccggcgcg tcgacagggt
     1381 tcccagcatt cccggcatga tcacgttctc cagggccgtg aagtcccgca ggagaaagtg
     1441 gaactgaaag atgaagccga cgtcgcggtt gcgatagcgg ctgagctgga tctcgtccag
     1501 cgaaccgatc tcccgcccgt ccaccaggat ccgtcccgag ctgggacgat ccaggcctcc
     1561 gatcaggccc agcagggtgc tcttgccgca gccgctctct ccggatacgg cgacggtgga
     1621 gccgggcatc acgtcgaggt tcacgccctt caggacctcc aggcgctcga gaccgttgtc
     1681 gaagtgccgg gccagcgacg tgacggacac gagcgggagc gggtcactca tagcgcagca
     1741 cctccgcggg gcggaaccgc gagaccgccc gggaagcccc cagcgccgcg ccggcgcagg
     1801 agaggacggc aaagaacacc accaggaacg cctccgtcgg atacacgtgg gagggaaccg
     1861 acgagagata gaagtaggcc ggggagaaga tggcgaagtc ctcgccgccc aacgccagga
     1921 acggcatggc cagcaggcgg gccatcccca ggacaccgtt cacgatcccc tgaacgacgt
     1981 cgaacacgcc gttgacgttc acggccagcg ccagaccgac gatcagtccg atgacccctc
     2041 cggccaggcc gatgaagagc ccctgaagga tgaacacgcc ctggatccgt tggggcggga
     2101 caccgacggc cttcagcacc gcgatctcct ccatccgctc atggacgctg cggcggaggg
     2161 agtggtacac gttgaagccc acgaccacga agatgaggcc gacgaggacc atcatcatga
     2221 gcttctccac gaacagcgcg tcgaagaagg actt
//
LOCUS       DASRZS010000210         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3197443, whole genome shotgun sequence.
ACCESSION   DASRZS010000210 DASRZS010000000
VERSION     DASRZS010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3197443"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFH83_05290"
     CDS             complement(<1..237)
                     /locus_tag="VFH83_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485809.1"
                     /translation="MHSRLLLPLLLCPLVLLGGCWNGRLLFFFEQPYWVSLGDDRPLR
                     LGLDREALRDGYLPSLVIPAVTEDPLTRLTRELSR"
     gene            320..1291
                     /locus_tag="VFH83_05295"
     CDS             320..1291
                     /locus_tag="VFH83_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HET6485810.1"
                     /translation="MVSYKELGLSNTRGMFKKAMAGKYAVPAYNFNNMEQLQAIIQGC
                     INSKSPVILQVSKGARKYANQVLLQYMAQGAMEMIKASGVSIPVALHLDHGDSYELCV
                     SCIESGFSSVMIDGSAHPYEKNVELTKKVVDFAHKHDVSVEGELGVLAGMEEEVSSEK
                     SHFTNPNDVEDFVKKTGVDSLAISIGTSHGAYKFKVKPGEPMPTLRFDILEEVERRIP
                     GFPIVLHGASSVIPEYVEMINRYGGKIEGAAGIPEDQLRKAARSAVCKINIDSDGRLV
                     MTAMIRKAFAEDPKNFDPRTYLGPAREELTKLIERKNRDVLGSAGQM"
     gene            1327..1947
                     /locus_tag="VFH83_05300"
     CDS             1327..1947
                     /locus_tag="VFH83_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6485811.1"
                     /translation="MEEKKERAIEKARDFFHRHGYRGASLSALIAELGISKPTFYNYF
                     RNKQELFYTVMLETFNEFQYRYNQQARAAANGMQKLDLFITTLAWFLDTYPIFRDLYK
                     PGNDLMSRWEQSRYCREFYAESVEIVRSILEQGVEEGIFSPALDPPASAPLVYAVALS
                     ALTADPRQHRAPNGPEYKIDAVALVGLLGRGVLSREDLPGPGGDSP"
     gene            complement(1952..>2463)
                     /locus_tag="VFH83_05305"
     CDS             complement(1952..>2463)
                     /locus_tag="VFH83_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485812.1"
                     /translation="LILILRTIIGFSYTGAWLFICLFLRDQFLPLFVSLTGFYLFRKL
                     LAVPTSEEGAFLVVFAYLAGFLSLINLTDALRYWGAWDASTLFLLPAARISQALIVAM
                     IARRYYPWEGRDAALFGCIIAILAAGLTLSAYLLLLNRPGWAIVLSIAPVLGSIVGFS
                     LRFPRAVRG"
BASE COUNT          540 a          746 c          805 g          372 t
ORIGIN      
        1 gcgcgaaagc tcgcgggtaa ggcgggtcaa cggatcctcc gtcacggccg ggatgaccag
       61 ggacgggaga tagccgtcgc ggagcgcctc acggtcgaga ccgagccgca gaggtcggtc
      121 gtcccccagg gagacccagt acggctgctc gaagaaaaag agcaggcggc cgttccagca
      181 tccgccgagc aacacgagcg ggcacaggag gagcgggagc agcagtcggg aatgcacggg
      241 tgatcgggta cctcggccct tgacagtgta gggtcatggg cggatattag tccagaggtt
      301 ttccccaaaa ggagacggaa tggtcagcta caaggaactc ggcctgtcca acactcgagg
      361 catgttcaag aaggcaatgg cgggcaagta cgccgtgccc gcgtacaact tcaacaacat
      421 ggagcagctc caggccatca tccagggctg catcaacagc aagtctcccg tcattctgca
      481 ggtctccaag ggtgcccgga aatacgccaa ccaggtcctg ctgcagtaca tggcccaggg
      541 cgccatggag atgatcaagg cgagcggcgt ctccattccg gtggcacttc acctggatca
      601 cggggattcc tacgagctgt gcgtctcctg catcgagagc ggcttctcgt cggtcatgat
      661 cgacggctcg gcccacccgt atgagaagaa cgtggagctc accaagaagg tcgtagactt
      721 cgcgcacaag cacgacgtgt ccgtggaggg ggagcttggc gtcctggccg gcatggaaga
      781 agaggtatcc tcggagaagt cccacttcac caacccgaac gacgtggaag acttcgtcaa
      841 gaagaccggc gtggacagcc tggccatctc catcggcacc tcccacggcg cctacaagtt
      901 caaggtgaag cccggagagc ccatgcctac gctgcgcttc gacatcctgg aagaggtgga
      961 gcgtcggatc cccggtttcc ccattgtcct gcacggcgca tcctcggtga tcccggagta
     1021 cgtggagatg atcaaccgct acggcggcaa gatcgaggga gcggcgggga ttcccgagga
     1081 ccagctccgc aaggcggccc gttccgccgt gtgcaagatc aacatcgact cggacggcag
     1141 gctggtgatg acggccatga tccgcaaagc cttcgccgag gatcccaaga acttcgaccc
     1201 gcggacctac ctgggcccgg cgcgggagga gctgaccaag ctgatcgagc ggaagaatcg
     1261 cgacgttctg gggagcgccg gtcagatgta ggcccggtcc gattcggcgg cctcgcggcc
     1321 attcatatgg aagagaaaaa agagagggcc atcgagaagg cccgagactt ctttcatcgt
     1381 catggctacc gcggggcctc gctctccgcg ctgatcgcgg agctaggtat ctccaagccc
     1441 accttctaca actacttccg caacaaacag gagctgttct acacggtgat gctggagacg
     1501 ttcaacgagt tccagtaccg ctacaaccag caggcccggg cagcggccaa cggtatgcag
     1561 aagctggacc tcttcatcac cacgctggcc tggttcctgg acacctaccc catcttccgg
     1621 gacctgtaca agccgggcaa cgacctgatg tcccgatggg agcagtctcg ctactgccgg
     1681 gagttctacg cggagagcgt cgagatcgtc cgatcgatcc tggagcaggg cgtggaggag
     1741 ggcatcttca gcccggcgct ggatccgccg gcctcggcgc ccctggtgta cgccgttgcc
     1801 ctctcggcgc tcacggcgga cccgcgccag catcgtgctc ccaacgggcc ggagtacaag
     1861 atcgacgcgg ttgccctggt gggcctgctg ggccgaggcg tcctcagtcg cgaagacctg
     1921 ccaggcccgg gcggcgatag cccctgagcc tctatccccg gaccgccctg gggaatcgga
     1981 gagagaagcc gacgatagag cccagcacgg gggcgatcga gagaacgatg gcccacccgg
     2041 ggcggttcag aagcaacaga taggctgaca gcgtgagtcc ggccgccagg atcgcgatga
     2101 tgcacccgaa tagagcggca tcccgacctt cccacgggta gtagcgcctt gcgatcatgg
     2161 cgacgatgag cgcctgagag atgcgcgcgg ccgggaggag aaagagcgtc gaagcgtccc
     2221 aggcacccca gtaccgcagg gcgtccgtca ggttgatcag ggacagaaac ccagcgaggt
     2281 acgcgaagac gacgaggaat gccccctcct ccgaggtcgg gacggcgagc agcttcctga
     2341 acagatagaa gcccgtgaga gagacgaaga ggggcaggaa ctggtcacgc agaaaaaggc
     2401 agataaagag ccaggcgccg gtgtaggaga aaccgatgat ggtccggaga atcaggatca
     2461 gca
//
LOCUS       DASRZS010000211         3910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2425356, whole genome shotgun sequence.
ACCESSION   DASRZS010000211 DASRZS010000000
VERSION     DASRZS010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2425356"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFH83_05310"
     CDS             <1..226
                     /locus_tag="VFH83_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485813.1"
                     /translation="TTSAYMEETGFTPGLRQYGVCDDTWSVLTYPGMLFERKVGGGRL
                     LINLLSQNRSIYLRCLAYLGEQGSARSSTR"
     gene            533..1786
                     /locus_tag="VFH83_05315"
     CDS             533..1786
                     /locus_tag="VFH83_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6485814.1"
                     /translation="MRRTLVAALAALVLMGMGGSLLFAADKAASISVWSWKSGDFWEK
                     VIIPTMKDKYNISVDNLVIGDYGQYTTKLKVAASAGELPDVLGLDPPDYLLYMRDYLA
                     PLDAYYVRDFGSNWKNRYVKSLLDQALLASNNGEMLAVLMGISEYFPWYNTKVFKDLG
                     ITQLPRNLNDLAAASKKLRAAGYGPLAFGGADEWQSNDLFRDIAADFDPKAVENAEKG
                     TMKWTDPVFVNVLKTVKRMVDLGIFQDGVWGDHMYMTLYDLFNQGKFGMARLGSWHAT
                     VTTPDFEAPMGISGFDYNGDGKVAPSIGSPDVAWTITKDSPNKDAAWKLIRWLGFDGG
                     EWLTTMVGLTSQNKDFSQSHLPDKMKPYWDLTFKFAQTAVRRNSLYAELSTAQAKAVQ
                     GVGTGTMTPEQAAKQLQSDFDAIPR"
     gene            1859..2737
                     /locus_tag="VFH83_05320"
     CDS             1859..2737
                     /locus_tag="VFH83_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485815.1"
                     /translation="MTRTRRSTRDVEGYLFVAPALLFFLAFIVYPIVFNVGISFFTWR
                     GIGAKFTYVGLGNYLRFFTSPITSRVGRNTGLLLAVMVPAHIAVGLIVAYLLNLRVRL
                     TAIYRILFFIPLVIPGVVTASVFGMLLEPSFGVLNTLLRSLRLGILARNWLGDTTLVI
                     WSIIVVTSWSFVGYAMVVYLGGLQGIPDEIREAAVLDGTNEPQMFLFVVFPLLKGCHA
                     TLVILGVLGVFRFFDIVWILTEGGPAQTSEVPATLIYRSAMMESQTGYAGAISVVVLI
                     TCIFLAVLLLGSFRRR"
     gene            2752..3600
                     /locus_tag="VFH83_05325"
     CDS             2752..3600
                     /locus_tag="VFH83_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485816.1"
                     /translation="MRTPSGRGARRAVSVHVILLVLVIPYASVFGFWAIHSFQRAATQ
                     AVQSEASGFQSAPGGLLNYLRVLQDPLIPRMLKNSVVVTGGSLILLFLSSSLAGFAIS
                     KLNFRGKNQIFVLLLTGLMLVPAVILTPTFQLLKFTRLLNTYFALIGPYVALEIPIGT
                     LIMKNFTDEIPGELLEAARIDGARSILVWYRIIVPLSVPAFIVVGLLGFLAFWNDYLL
                     AISFMTKSAMVMATMAPSRFMIRYGGDTSLILAASMVISLPALVIFIVLQRYLVSGMI
                     AGAIKA"
BASE COUNT          665 a         1265 c         1216 g          764 t
ORIGIN      
        1 caccacctcc gcgtacatgg aggagaccgg gttcacccca gggctgcgcc agtatggtgt
       61 gtgcgatgac acgtggtccg tccttaccta tcccgggatg ctcttcgagc ggaaggtcgg
      121 cgggggcagg ctgctcatca acctgctgag ccagaaccgg tcgatttacc tgcgctgtct
      181 tgcctacctg ggtgagcagg gatccgctcg gtcgtcgacc cgatgaaggc cacgagggaa
      241 ccggccttga cttccggctg cggaatgcta ccgttgaccg ggccaagcgc gtaacttcat
      301 tacgcgtcgg attcgagagt gcatggtgcc aggagacctc ctgaggttcc gctcgtcatc
      361 ccatccccgc ggccacccgg cttggctcgt ccaccccata gacaacgacc gcacccagaa
      421 cgacccgccc ggagagcgga acgggtccgt gacgggacgc gccggcagac cgtggctttg
      481 ggagaccgct gacctcagag cgaggcatgg gccaacgaga ggagggtcgt tcatgagaag
      541 gactctggtg gccgcgcttg cggcgctggt gctgatgggc atggggggat cgcttctctt
      601 cgccgccgac aaggcggcgt cgatcagcgt ctggagctgg aagtccggcg acttctggga
      661 gaaggtgatc atcccgacga tgaaagacaa gtacaacatc tcggtagaca acctcgtgat
      721 cggcgactac ggccagtaca ccaccaagct caaggtcgcc gcgtccgccg gcgagctgcc
      781 ggacgtgctg ggtctggatc ccccggacta tctcctgtac atgcgggact acctcgcgcc
      841 tttggatgcc tattacgtca gggattttgg ctcgaactgg aagaaccggt acgtcaagtc
      901 gctcctggac caggcgctcc ttgcctcgaa taacggggag atgctcgcgg ttctcatggg
      961 gatcagcgag tatttcccct ggtacaacac caaggtcttc aaggacctgg gaatcacgca
     1021 gctacctcgg aacctgaacg acctggccgc ggcttccaag aagctgcgcg cggcgggata
     1081 cgggcccctg gcgttcgggg gggcggatga gtggcaatcc aacgacctgt tccgggatat
     1141 tgccgctgac ttcgatccca aggccgtcga gaacgccgaa aaggggacga tgaagtggac
     1201 ggacccggtc ttcgtcaacg tcctgaagac ggtcaagcgg atggtcgacc tggggatctt
     1261 ccaggacggc gtttggggcg accacatgta catgaccctc tacgacctgt tcaaccaggg
     1321 caagttcggc atggcgcgtc tcggatcgtg gcacgccacg gtgaccactc ccgatttcga
     1381 ggcgcccatg gggatctcgg ggttcgacta caacggtgac gggaaggtgg cccccagcat
     1441 cggaagcccc gacgttgcct ggacgatcac caaggactcg ccgaacaagg acgccgcgtg
     1501 gaagctcatc cgatggctcg ggttcgacgg cggagaatgg ctgacgacga tggtcggcct
     1561 gacgtcccag aacaaggact tctcccagtc gcacctgccc gacaagatga agccgtactg
     1621 ggacctgacc ttcaagttcg cccagaccgc cgtgcgccgc aactcgttgt atgccgagct
     1681 gtccaccgcc caggcaaagg cggtccaggg cgtcggcacc ggcaccatga cgcctgagca
     1741 ggccgcgaag cagctccagt cggacttcga cgcaatcccc cggtagccaa acccgtctcg
     1801 cgccgtcgcg ctcggctctc gagcgcgacg gcttctcgct cccggcagga ggcatcgtgt
     1861 gaccaggacc cgcagatcga cacgcgacgt ggagggctac ctctttgtgg cccctgccct
     1921 gctcttcttc ctggcattca tcgtctaccc catcgttttc aacgtgggga tcagtttctt
     1981 cacctggcgc ggtatcgggg cgaagttcac ctacgtgggg ctcggcaact acctccggtt
     2041 cttcacgagc cccatcacct cgcgcgtcgg caggaacacg ggtctgctcc tggcggtgat
     2101 ggtgcccgcc catatcgccg tcggcctgat tgtggcctac ctgctgaacc tgcgggtccg
     2161 gctcacggcg atctaccgga tcctcttctt cattccgctg gtgatccccg gcgtggtcac
     2221 ggcgtccgtg ttcgggatgc tcctggagcc gtcctttggc gtcctgaaca cgctcctgcg
     2281 ttctctccga ctcggcatcc tcgcccgaaa ctggctggga gacacgaccc tggtgatctg
     2341 gagcatcatc gtcgtcacga gctggtcctt cgtgggatac gccatggtgg tatacctggg
     2401 agggttgcag ggcattccag acgagatccg tgaggccgcg gtcctggacg ggacgaacga
     2461 gccccagatg ttcctctttg tcgttttccc gctcctgaag ggctgccatg ccacgctggt
     2521 gattctcggg gtgcttgggg tcttccgctt cttcgatatc gtctggatcc tgaccgaggg
     2581 cggtcccgcg cagacctccg aggtgccggc gaccctcatc taccgcagcg ctatgatgga
     2641 gagtcagacc ggctacgcgg gagcgatctc ggtcgtggtg ctgatcacgt gcatcttcct
     2701 ggccgtcctc ttgctgggaa gctttcgcag gcgttagggg agagagacgc catgagaacg
     2761 ccttccggcc ggggcgcgcg ccgcgccgtc agcgtgcacg tcatcctgct cgtgctcgtg
     2821 atcccgtacg cctccgtgtt cggattctgg gcgatccact cgttccaacg ggccgccacg
     2881 caggccgtcc agtccgaggc ctccggcttc cagagcgcgc ccggcggcct cctcaactac
     2941 ctgcgggtgc tccaggatcc gctcatcccc cggatgctca agaacagcgt ggtcgtgacc
     3001 gggggctccc tgatcctcct ctttctctcc tcctcgctgg cgggctttgc catctcgaag
     3061 ctcaactttc gagggaagaa ccagatcttc gtgctcctct tgacggggct catgctcgtg
     3121 cccgcggtca tcctcacccc cacctttcag ctcctgaagt tcacgaggct gctcaacacc
     3181 tacttcgccc tgatcgggcc ctacgtcgcg ttggagatac cgatcggcac gctcatcatg
     3241 aagaacttca ccgacgagat cccgggagag ctcttggagg cggcgcgcat cgacggagcg
     3301 cgttccatcc tggtctggta ccggatcatc gtgccgttgt ccgttcccgc gttcatcgtg
     3361 gtcggattgc tgggcttcct ggcgttctgg aacgactacc tcctcgcaat ctcgttcatg
     3421 accaagagcg ccatggtgat ggcgaccatg gcgccctcgc gattcatgat ccgctatggc
     3481 ggcgacacct cgctcatcct ggccgcgagc atggtcatca gcctcccggc cctggtgatc
     3541 ttcatcgtcc tgcagcgata cctggtgtcc ggcatgattg ccggcgctat caaggcgtag
     3601 cggttgaagc ggcaaccggc gcggttgcga aagagactgc ccccaggccc tgcccatggt
     3661 aggattcgcc ccaagtttca ccaaggactg tcccttacgg tgacgtggcg ccttgcactg
     3721 gggagcttga tgccgttgga caggacgatc tgaagaccgc ccgtcggaac ggggacggtg
     3781 gagcggtgac ccaggctcgc ggtggccgaa ggcgtctgga tctgccccag cgctgggaat
     3841 cgcggaaagc ctcggattct cgatctccgg ctctacggcg gcctacacga acaacgccgt
     3901 gggcaacggc
//
LOCUS       DASRZS010000212         5230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1273127, whole genome shotgun sequence.
ACCESSION   DASRZS010000212 DASRZS010000000
VERSION     DASRZS010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273127"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..839)
                     /locus_tag="VFH83_05330"
     CDS             complement(291..839)
                     /locus_tag="VFH83_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011746488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485817.1"
                     /translation="MRIAYADPPYPGCAKLYRDQADYAGEVDHAELVRRLEAEFDGYV
                     LHTSSVALPLVLPLLSAPYRVMAWVKPFAAFKRNVSVAYAWEPVVVRACRKPVVAHRV
                     IMRDWFAESITLRKGLTGAKPPNLIRWAFEMVGAQLGDDLADLYPGTGIVGVTWDEWC
                     RERQAEVPAPPASGTREPGGET"
     gene            complement(880..1326)
                     /locus_tag="VFH83_05335"
     CDS             complement(880..1326)
                     /locus_tag="VFH83_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HET6485818.1"
                     /translation="MSLDLVPCTMKDAMEFVAQHHRHHRKPAGGLFAVGVADGADLVG
                     VAIVGRPIARMLMDGWTAEVIRVCVLEGHRNACSMLYGACWRAARALGWRKLVTYTLP
                     EEGGASLRAAGWRCLGEAGGGKWSRANRPRVDDHPTQVKWKWEPPR"
     gene            complement(1329..1478)
                     /locus_tag="VFH83_05340"
     CDS             complement(1329..1478)
                     /locus_tag="VFH83_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485819.1"
                     /translation="MSDALREFESATMTACHDLSGAYERRTAEEAKAALEVAILAALR
                     AARRE"
     gene            complement(1523..1858)
                     /locus_tag="VFH83_05345"
     CDS             complement(1523..1858)
                     /locus_tag="VFH83_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018865117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RyR domain-containing protein"
                     /protein_id="HET6485820.1"
                     /translation="MTNEQIAKVCHEANRAYCATLGDTSQVPWEQAPEWQRTSAVNGV
                     EHARDPAAKPSDSHDGWLKEKAATGWKYGPVKDPEKKEHPCFVPFADLPEEQKRKDAL
                     FLAVARALM"
     gene            complement(2101..2370)
                     /locus_tag="VFH83_05350"
     CDS             complement(2101..2370)
                     /locus_tag="VFH83_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485821.1"
                     /translation="MLQDTPLPDDRCPTCGHAFDTATPMPSNDRSVPAPGDLAVCIAC
                     GQILVFNTQMKYEIFLWNEELDPETKGLLRRAQDFIRARLPRIEF"
     gene            complement(2559..2969)
                     /locus_tag="VFH83_05355"
     CDS             complement(2559..2969)
                     /locus_tag="VFH83_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485822.1"
                     /translation="MTTKFQKITPEVEEILQESVFRCAFKIKKTHRKACSYLPAHVAL
                     ATLRAATIGLVKDIEDELRELEGISAPQIAEAIAVSDRMAEKFLAEIRQKKAESKAMR
                     AAQNQAIEAVKATLMQAAGVPQESKSKKGFFPDA"
     gene            complement(2966..3490)
                     /locus_tag="VFH83_05360"
     CDS             complement(2966..3490)
                     /locus_tag="VFH83_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485823.1"
                     /translation="MNRIPAVALCRDVLRWKPNASGFVHLDPSEVDYLQSLAREAMAK
                     AAKRPAKKLATGRMKPTRASKKAMEKAVYAAVILRDPVCQVSRGAFGPCEGFLEIDHQ
                     WGRGKAPTSVGNCRRLCEKHHHMKTDSVPDRIAWLDDFRYWALNKGYLTEADKARKMV
                     MLESAQHPEREKAP"
     gene            complement(4088..5041)
                     /locus_tag="VFH83_05365"
     CDS             complement(4088..5041)
                     /locus_tag="VFH83_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485824.1"
                     /translation="MAAALTEEREQRALLRKYVAEFMIPGDDYGTIPGTERKGLFKPG
                     AEKLLDLFRCRPEYEFLTREVNHETGLYYYEIRCKAVSRETDTVLNEGVGSASSYESK
                     YRYRNALRKCPACGKETIRRSKFKMRSEDHGTPPCWWCDARNGGCGKEYTHNAPEIVK
                     QEAGKIQNPDLADMANTVLKMAKKRAQVDCAIALARVSDLFTQDIEDLPDLAGAAESL
                     QEPVEPPERQKPPPRASPPAEPPSEAPSGPSEPPREKNPVEKLWDKAVAKWGDEGTKA
                     RWRAAAAKVFDGKRPPPSKDWREVDVLDMEAVLFEPTDVPF"
BASE COUNT          755 a         1793 c         1659 g         1023 t
ORIGIN      
        1 gccttgaggg cgcggagttc acggcgcagt gcatcgccgg ttggattctt tccgcttacc
       61 gtcttgatgc tggcgtagtc ctcgacaagc tcggtcagcc gtggcgctat cttgtcagcc
      121 atgctcccct ccaggcgtag gtggatccgc ggctagttcg gcgtgcatct ttcgccgggc
      181 cttgcgacgc aacgccttcc gttcgggccc agccacgccc tcgaagcggt cgcgccagtc
      241 ccggcagcgg agttcggtcc cctcctgctc cttgcgcttg aattcgtctt tcacgtctcc
      301 cctccaggct cccgggtccc gctcgctggc ggggcgggga cctcggcttg tcgttcacgg
      361 caccattcat cccaagtgac gccgacgatg cccgttccgg ggtagagatc cgctaggtcg
      421 tcgcccagct gcgcacccac catctcgaag gcccagcgga tcaagttcgg cggcttggcg
      481 cccgtgagcc ccttccgcag cgtgatcgac tccgcgaacc aatcacgcat gatcacccga
      541 tgcgcaacga ccggcttgcg gcaggctcgg actaccaccg gctcccaggc gtaggcgacc
      601 gacacgttgc gcttgaacgc ggcgaagggc ttgacccagg ccatcacgcg ataaggcgca
      661 gagaggaggg ggaggacgag cgggagcgcc acgctggacg tgtgcagcac gtagccgtcg
      721 aattccgcct ctaggcgcct caccagctcg gcgtggtcca cctcgccagc gtagtcggct
      781 tggtcccggt acagtttcgc gcaccccggg tacggtggat ctgcgtaggc gatcctcacc
      841 ggggcgtctc cttggtctcg gggacggggg gcacatggcc taccttggcg gctcccactt
      901 ccatttaacc tgcgtcgggt gatcatcgac gcgtggccgg ttcgctctcg accacttgcc
      961 gcctcccgcc tcacctaggc acctccagcc cgccgcccga agcgaagcac ccccctcctc
     1021 agggagcgta taggtgacca gcttccgcca ccccagcgcc cgagcagctc gccagcaggc
     1081 gccgtagagc atcgaacagg cgttgcggtg accctccagc acgcacaccc ggatgacctc
     1141 tgccgtccac ccgtccatga gcatccgggc aattggccgg cccacgatgg cgacgcccac
     1201 gagatcggcc ccgtccgcca cacccaccgc gaacagtccg ccggcgggct tgcggtgatg
     1261 gcgatggtgc tgcgccacga actccatggc gtctttcatc gtgcagggga cgaggtcgag
     1321 gctcacggct attccctcct tgctgcccgc agggccgcga ggatcgcgac ctctagggcg
     1381 gccttggctt cctcggcggt gcgtctctca taggcgccgg ataggtcgtg gcacgcggtc
     1441 atcgtcgccg actcgaactc gcgcagcgcg tcgctcacct cctccgggag cgcctccgtc
     1501 tctcgctccg gtacgggggc ggttacatca gcgcgcgggc cacggccagg aagagagcgt
     1561 ccttccgctt ctgttcctcc gggaggtccg cgaagggcac gaagcacggg tgctccttct
     1621 tctccgggtc cttcaccggc ccgtacttcc atcccgtcgc cgccttctcc ttgagccacc
     1681 catcgtggct gtcggagggc ttcgccgccg gatcccgggc gtgctccact ccgttcacgg
     1741 cgctcgtccg ctgccactcc ggagcctgct cccacgggac ctggctcgtg tcgcccagcg
     1801 tcgcgcagta cgcccgattc gcctcgtgac acaccttcgc gatctgctcg ttcgtcatcg
     1861 gatgctcctt cctgcggtgg gtgctgcgtt gaaagctctc ggggcgccgc gctctccggg
     1921 ctcgcggcca ggacgaggcg aagctccttc gccagtcgga acatgaccgg gccctcatag
     1981 gttcggaccg aatgaagcgg ggcggcgatc cgctcccact cctgcgccag attcagcatc
     2041 gtctctctca gttcagactc gctcatatct ctggctccct ccggtgctcg gggtgctccc
     2101 ctagaactcg atcctgggta gacgggcccg gatgaagtcc tgggccctac gcaggagtcc
     2161 cttggtctcc gggtccagct cttcgttcca gaggaagatc tcgtacttca tctgggtgtt
     2221 gaagacgagg atctggccgc aggcgatgca taccgccagg tctccgggag ccgggacgga
     2281 tcggtcgttg ctaggcatcg gggtagcggt atcgaaggcg tggccgcagg tggggcagcg
     2341 gtcgtccggt agaggcgtgt cctggagcat ggcgttctcc cttggtgagt gggcggggtc
     2401 gggcccattc ccgacccggt cctttggtcg ctggccgcgg tggagtacgg gccatatgag
     2461 ggccgaatgc tgcacccgcc caggtagcac agagaactgg cctgggaatc cacggctacg
     2521 acctgacgag gacgtcgccc atgtccccga gaactcccct aggcgtcggg gaagaagccc
     2581 ttcttcgact tggactcctg cgggacgccg gcagcctgca tcagcgtcgc cttgaccgcc
     2641 tcgatggcct ggttctgggc ggcgcgcatg gccttggact ctgccttctt ctgccggatc
     2701 tcggccagga acttctccgc catccgatcg gagaccgcga tggcctcggc gatctggggg
     2761 gcggagatcc cttccagctc ccgcagctcg tcctcgatgt ccttgacgag cccgatggtg
     2821 gctgcccgga gggtggccag ggcaacgtgg gccggcaggt aggaacaggc cttccggtgg
     2881 gtcttcttga tcttgaaggc gcaccggaac acggactcct ggagaatctc ttcgacctcg
     2941 ggcgtgatct tctggaactt cgtggtcatg gtgccttctc cctttctgga tgctgcgccg
     3001 actcaagcat aaccatcttc cgcgccttgt cagcttctgt caagtagccc ttgttcaacg
     3061 cccagtaacg gaaatcatcc agccaggcta tccggtccgg gacactgtcc gtcttcatgt
     3121 ggtggtgctt ctcgcagaga cggcggcaat tgcccacgct ggtcggcgcc ttcccccggc
     3181 cccactggtg gtcgatctcc aggaagccct cgcatggccc gaaggctccc cgggagacct
     3241 ggcacactgg gtcgcgcagg atgacggccg cgtagacggc cttctccatg gccttcttgc
     3301 tggccctggt gggcttcatc cggcccgtgg ccagcttctt cgctggacgc ttggccgcct
     3361 tggccatggc ctcccgggcc aggctttgga ggtagtcaac ctcggacggg tccaggtgaa
     3421 cgaacccgga ggcattcggc ttccaccgga gaacgtcccg gcacagggca acggcgggga
     3481 tgcggttcac gggcgcctcc agggaaagag tccggggttc gacctgggga ggtccacaga
     3541 ggagaaagcc agaaggcctc cgtggcggac ccaagtccta tttaccggca ccccggatgg
     3601 ccggtccagc gccccgctat ggaaccaccg gagtctgtcc atgagcgcgt tcccggtggt
     3661 gcttgaagca gtaccactct acctccagcg gcttcgagta gtcagcgtgg tgcgcctgtg
     3721 agtttggcga gccgcagatc gcgcagggca atcgaagaac aaggcccttg atcatggccc
     3781 accggaggcg tgctctagcc cggaagcggt cggggtgccg acggcgagca gtacgctggt
     3841 actcagcggc gcgttgcttc ctttcgggcc tctggttgcg ttctctctcg tagaggaccc
     3901 gcatccccct ggtctccgcg tagtgccgtt ggacatcact ccgggtgcat tctttgcatt
     3961 ttccgaggtg cccatctgcc attcccggat gtctgtagaa ctcttccaac ggcttggctt
     4021 gcccacactt gaagcacgtc ttcatggacc caaacgctac tacactccgg tcacgccgtc
     4081 aacccttcta gaaggggaca tctgtcggct cgaacagcac ggcctccatg tccagcacgt
     4141 ctacctctct ccagtccttc gacggcgggg ggcgcttgcc atcgaagacc ttggccgcgg
     4201 ctgccctcca gcgcgccttg gtgccttcgt ccccccactt ggcgacggcc ttgtcccaca
     4261 gcttctccac cgggttcttc tctcgaggag gctcgctcgg accgctaggc gcctcgctgg
     4321 gaggctctgc gggaggggag gctctgggag gcggcttctg gcgctccgga ggctccacgg
     4381 gctcctgtag cgactccgcg gcccccgcca ggtcaggcaa gtcctcgatg tcctgggtga
     4441 aaaggtctga gacgcgggcc aaggcgatgg cacagtccac ctgggccctc tttttggcca
     4501 ttttcaggac cgtgttggcc atgtccgcga ggtccgggtt ctggatcttc ccggcctcct
     4561 gcttcacgat ctccggggcg ttgtgggtgt actccttccc acagcccccg ttccgggcgt
     4621 cgcaccacca gcacggcggt gtcccgtggt cctcggaccg catcttgaac ttggaccggc
     4681 ggatggtctc cttcccgcag gcggggcact tccggagagc gttccgatac ctgtacttgg
     4741 actcgtagct ggaggccgag cccacaccct cgttgagcac ggtgtcggtc tcccgggaga
     4801 cggccttgca ccggatctcg tagtagtaga gccccgtctc gtggttcacc tcccgggtca
     4861 ggaactcgta ctcgggccgg cagcggaaga ggtccagtag cttctccgcg cccggcttga
     4921 agagcccctt ccgctccgtg ccggggatgg tcccgtagtc gtcgcccggg atcatgaact
     4981 cggccacgta cttccggaga agagcgcgct gctcacgctc ctcggtgagt gctgcggcca
     5041 tcatcagggc ccgaggggtg cgaacggcga gcttgtcccc tggaagcgaa ggcatgggct
     5101 gctccgggtg ggtaagggca tccgttggcg cggtggccac gctgtccgga ccctcctgca
     5161 ccggctggtc acttcgtcgc gaggtcgtca tcgagcttct ccggtgtgac gtagggtgtc
     5221 tccccggcct
//
LOCUS       DASRZS010000213         3091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3199690, whole genome shotgun sequence.
ACCESSION   DASRZS010000213 DASRZS010000000
VERSION     DASRZS010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3199690"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VFH83_05370"
     CDS             <1..997
                     /locus_tag="VFH83_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HET6485825.1"
                     /translation="GDPSPRVVRGPGYAATEDAVALSADKSIQELLQSRRPVVVTDAP
                     AEPGSLRPRSFVAAPIQIGETVVGVLRVDGMQPRRFGPRDATRLEAFTHHAAAAIANA
                     RLYEKAQAEILERRLTEQKIVSSLKEKEVLLKEIHHRVKNNLQVISSILYLQSRTITD
                     SRVLEAFQDSRTRVKSMALVHEMLYRSPDLARIDLAAYGQSLANFLSSTQAAANQHVS
                     MQVEIEPIALSVDAAIPCGLILNELVTNCLKHAFTGRDQGTIRISVVANGANTFVLRV
                     ADDGVGLPDGFDFQKTESLGLQIVGTLVSQLTGTIELERGQGTCFKITFPDPDAK"
     gene            1123..2403
                     /locus_tag="VFH83_05375"
     CDS             1123..2403
                     /locus_tag="VFH83_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET6485826.1"
                     /translation="MRSPSRLLVCALLCMVLVSPVFAAKVHLTFLYFGNPDEQKQWAN
                     TCKMYSDSQDKIEVEYIPCANDAYTQKILTLFASHDSPDTFYARNGETPVLLKNNRLL
                     DLTQLIKADPTFKLTDYVNFDQIDKPYIRDGHIYAIHDNDNPMVLYFNRTMLDSLGMP
                     NPYDLYTKGQWTWDQFTKIVTKVRDSSTPAKPLYTFIQDAWFGPNYLWAVSNGVTSFY
                     KNGKAAYSDPAVLEALTWNLQRLNDKTILYGASSGKGMDGSQLFQAGQIAFMSAGRWM
                     VPTYKRITGFKWDVVPFPKGPKATQYYSYTPGYTAFAISAETKHPNEAWQFFKWWVGK
                     AGQTQNWAKGGNCLPTIKGLEQTVLDDVAYYPHPQVFLDIQKVSIWPDIAYAMNPKAT
                     KIVDDAFQQVYMLQGDPKKVMTQLDSDLNSGAAD"
     gene            2493..>3091
                     /locus_tag="VFH83_05380"
     CDS             2493..>3091
                     /locus_tag="VFH83_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485827.1"
                     /translation="MNPRRKRRLRETASAYLLLSPQLIGLLAFGALPILIALGISFTE
                     WDVIGPMKFVGLTSIVKELRHPYFLKSLINTLEYVGMNVPLSIGCALIASLLLIDARF
                     KVLFRALYFMPVVTSVVAGVMVWQWLYHPDFGPLNFLIHRVLGFPGKILWLGSTKTAM
                     LSIVMMNTWQTLGYGLVLFLAGLQAVPAVYYEAAIMDGAG"
BASE COUNT          586 a         1039 c          923 g          543 t
ORIGIN      
        1 gggcgacccg tcgccgcgcg tggtgcgggg gccgggctac gccgccacgg aggatgccgt
       61 tgcgctctcc gcggacaaga gcatccagga gctgctgcag tcccggcggc ccgtcgtggt
      121 gaccgacgcc ccggcggaac cggggtctct gcggccccgg tctttcgttg ccgctcccat
      181 ccagatcggg gagaccgtgg tgggcgtgct gcgcgtggac gggatgcagc cgaggcggtt
      241 cggcccccgt gacgccaccc gactggaggc attcacgcac cacgcggccg cggccatcgc
      301 caacgcccgc ctgtacgaga aggcgcaggc cgagatcctg gagcggcggc tgacggagca
      361 gaagatcgtc tcttcgctga aggagaagga ggtgctgctc aaggagatcc atcaccgggt
      421 gaagaacaac ctccaggtga tctcgagcat cctctacctc cagtcgcgca ccatcaccga
      481 cagccgggtg ctggaggcgt tccaggacag ccgcacccgg gtgaagtcca tggcgctggt
      541 gcacgagatg ctctaccggt cgccggatct cgcccggatc gatctggccg cctatggcca
      601 gagcctggcc aacttcctgt cctccacgca ggcggccgcc aaccagcacg tttccatgca
      661 ggtggagatc gagccgatcg ccctctcggt ggatgctgcc attccgtgcg gtctcatcct
      721 gaacgagctg gtcaccaact gtctcaagca cgcgttcacg ggccgggatc aggggaccat
      781 ccgcatcagc gtggttgcca acggcgccaa caccttcgtc ctgcgggtgg cggatgatgg
      841 ggtggggctc ccggacggct tcgacttcca aaagaccgag tccctgggat tgcagatcgt
      901 cgggaccctg gtgagccagc tcacgggcac gatcgagctc gagaggggcc agggcacctg
      961 cttcaagatc accttcccgg atcctgacgc gaagtagacg tccggccgcc tgcccgcgcg
     1021 agaaccgcgc ggccgtccca cggttttttc ttgacacctc ctgggttcaa tgttatagcg
     1081 gaggcgagcc aaaccgtacg aaatcgaaca aaggaggttc ctatgagatc accttcccgt
     1141 ctcctggtgt gcgcgctcct gtgtatggtg ctcgtttcgc cggtgtttgc ggcgaaagtg
     1201 cacctgacgt tcctgtactt cggaaaccct gacgagcaga agcagtgggc caatacctgc
     1261 aagatgtata gcgacagcca ggacaagatc gaggtggagt acatcccctg cgccaacgac
     1321 gcctacacgc agaagatctt gaccctgttc gcctcccatg actctccgga caccttctac
     1381 gcccgaaacg gcgagacgcc ggtgctcctg aagaacaacc ggcttctcga cctcacgcag
     1441 ctcatcaagg cggaccccac tttcaagctc accgactacg tcaacttcga ccagatcgac
     1501 aagccctaca tcagggacgg gcacatctac gccatccacg acaacgacaa ccccatggtg
     1561 ctgtacttca accggaccat gctggacagc ctggggatgc ccaacccgta tgacctgtac
     1621 accaagggcc agtggacgtg ggaccagttc acgaagatcg tcacgaaggt cagggactcc
     1681 agcaccccgg cgaagcccct gtacacgttc attcaggatg cctggttcgg ccccaactac
     1741 ctctgggcgg tctccaacgg cgtaacctcg ttctacaaga acgggaaagc agcttacagc
     1801 gacccggccg tcctggaggc gctcacctgg aacctccagc gcctgaacga caagaccatc
     1861 ctctacggcg cctcctcggg caagggcatg gacggcagcc agctcttcca ggcagggcag
     1921 atcgccttca tgagcgccgg ccgctggatg gtccccacgt acaagcggat cacgggtttc
     1981 aagtgggacg tggttccgtt ccccaagggc cccaaggcca cccagtacta ctcctacacc
     2041 cccggctaca cggcgttcgc catcagcgcc gagaccaagc atcccaacga ggcgtggcag
     2101 ttcttcaagt ggtgggtagg aaaagccggc cagacccaga actgggccaa aggcggcaac
     2161 tgcctcccca ccatcaaggg cctggagcag accgtcctgg acgatgtcgc ctattacccg
     2221 catccgcagg tgttcctcga catccagaag gtctccatct ggcccgacat cgcctacgcc
     2281 atgaacccca aggccaccaa gatcgtggat gacgccttcc agcaggtgta catgctccag
     2341 ggggatccca agaaggtcat gacccagctc gacagtgacc tcaatagcgg cgcagcggac
     2401 taggcctgcc gccgcccatc tcggtgcttc cggccgggga ccttcccgtc cccggccttt
     2461 cttccatcgg actccagtca gcaggtgaga ccatgaaccc caggcggaag cgaagactga
     2521 gagagaccgc gagcgcgtat ctgctgcttt ctccgcagct catcgggctc ctggcgttcg
     2581 gagcgctgcc catcctgatc gcgctgggca tcagcttcac ggagtgggac gtcatcgggc
     2641 ccatgaagtt cgtgggtctg acgagcatcg tcaaggagct gcgccacccc tatttcctga
     2701 agtccctgat caacacgctc gaatacgtcg ggatgaacgt gcccctgtcc atcggctgcg
     2761 cgctcatcgc ctccctcctc ctgatcgacg cgcggttcaa ggtcctcttc cgtgccctgt
     2821 acttcatgcc cgtggtcacc agcgtggttg cgggcgtcat ggtctggcag tggctctacc
     2881 atccggactt cggcccgctc aacttcctga tccaccgggt cctggggttc ccggggaaga
     2941 tcctctggct gggctccacg aagaccgcga tgctctccat cgtgatgatg aatacgtggc
     3001 agacgctggg atacgggctg gtgctcttcc tggcgggtct ccaggcggtg ccggccgtct
     3061 actacgaggc ggcgatcatg gacggcgccg g
//
LOCUS       DASRZS010000214         8556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2048641, whole genome shotgun sequence.
ACCESSION   DASRZS010000214 DASRZS010000000
VERSION     DASRZS010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8556
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048641"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VFH83_05385"
     CDS             complement(<1..643)
                     /locus_tag="VFH83_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485828.1"
                     /translation="MKTLASPIHDLAASYRLFLVDFPEELQSQHLEVPPRERAATFGG
                     LRRLRERIGAVYALAPAMAPRGERWLDREFCFRAIEAPVRMLWALGTCGEQTRGPWGS
                     TLRADTADLEARRRQAGVRDVRWATRVLERAGFGLRYRDNTEKEIPEYRGSSSVSLLS
                     PGDDGSMLRALAYYARRVPRNPKTRRGAILEVFLRADLRPLLPGYAAVLPHLPA"
     gene            804..1820
                     /locus_tag="VFH83_05390"
     CDS             804..1820
                     /locus_tag="VFH83_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6485829.1"
                     /translation="MSDSSGPLLEVAGLKKHFPIKKGFLKRTVGYVRAVNGIDFYIRR
                     GETLGLVGESGCGKTTTGRMIVRALKPTAGQILFHTNGTTTDLAPLEFRELKPFKKHI
                     QMIFQDPNSSLNPRMTILEIIGEPLIVNGVARGQELEDRVRELLKTVGLRAQFLRRYP
                     HAFSGGQRQRIGIARALALNPDLIVADEPVSALDVSIQAQILNLLQDLQEEFRLSYLF
                     IAHDLSVVEHISNRVAVMYVGQLVEMASTDELFPRPRHPYTEALLSAVPVPDPINKRK
                     RLILPGEVADPANPPPGCIFHPRCKYAREICSNEVPHLEEIEQEHFVSCHLARELSLK
                     GVKK"
     gene            1817..2875
                     /locus_tag="VFH83_05395"
     CDS             1817..2875
                     /locus_tag="VFH83_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014509950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6485830.1"
                     /translation="MSSPATVAQNTLIEVQNLKTHFVLDEGLLKAVDGVSLSIERGKT
                     LGLVGESGCGKSVTAQSIMRIVPPPGRIVQGKILYSRPGDGGSAGTVDLASLEASGTD
                     MRRIRGKDITMIFQEPMTSFSPLHTIGNQIMEAILLHRSRDPAEAREIATDMLAKVGI
                     PNPGQRIDQYPHELSGGMRQRAMIAMALSCNPSLLIADEPTTALDVTVQAQVIELMEG
                     LRQELGMSMLYITHDLGVIAEVADEVAVMYLGRIIERGSVMDIFYHAAHPYTRALLQS
                     VPRMGRRGQRRRLQAIRGTVPIPINLVDRCAFRTRCQHFERSKCMGSEPALVEIEKGH
                     WVRCVRYDELGEGDGALP"
     gene            complement(2888..4027)
                     /locus_tag="VFH83_05400"
     CDS             complement(2888..4027)
                     /locus_tag="VFH83_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485831.1"
                     /translation="MREKSRLTEDQLEELEQEDREEEEYYVASQWKLIWWKLRRHKLA
                     MGAAVALILLYLIVVFAEFLSPYTPLTRYSDYVYAPPNRVRLIGNGFLRPYIYGYNKT
                     VDPATYRRIFVIDPATKYPVKFFAHGERYKFWGLFRTDVHLVTSDGFPIFLLGTDRLG
                     RDVFTRILYGTRISLSIGLVGITFTFIFGLLLGGLSGFFGGVTDTIIQRSIDLLISIP
                     TIPLWMALAAALPRDWPVVRIYFGITVIFSIIGWTGLARVVRGKLLSLREEDFVIAAQ
                     VAGSSNLRIIGRHLIPSFASYIIVSLTLSVPNMILGETALSFIGLGMQPPAVSWGTLL
                     QDAQDLSALVHYPWLLTPCIFVVIAVLAFNFLGDGLRDSADPFAR"
     gene            complement(4024..5022)
                     /locus_tag="VFH83_05405"
     CDS             complement(4024..5022)
                     /locus_tag="VFH83_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485832.1"
                     /translation="MLSYIGRRILQLIPLLLVLSVLIFISIILPPGDYLSIYINQLKT
                     SGTQPGADEIARLTRQYGLDQSLPERYLLWMWNMVRYGSLGRSFEWNKPVTDLIGEQI
                     GLTILLSILSLLVTYALAIPIGIYSATHQYKIGDYTFTFLGFVGLSVPPFLLALALVY
                     FSFRYLGISVTGLFSPAYVDAPWSLAKFIDMAKRIWIPVLIVGLSGTAYTIRVMRACL
                     LDELRKQYVITARAKGVDERTLLFKYPVRVAINPIVSTVGWLLPALISNTPLVAIVLN
                     IPTTGPMLLRALLDRDMYLAGSFLLILSVMTVIGTLISDVLLAVLDPRIRYGGIGE"
     gene            5364..7259
                     /locus_tag="VFH83_05410"
     CDS             5364..7259
                     /locus_tag="VFH83_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6485833.1"
                     /translation="MAKRVLLALLALAILLPAAFAQAPGKYPIPATFKQSPMLDDLVK
                     SGKLPPVKDRLPKHPLVVGLGTDMSATDLPDWQVGKYGGTERMPRIWPTADYGMFIQD
                     NQPIIRGVGLAQEPFYGNVVESYDVSADQKTFTFHMRDGMKWSDGQPVTSDDVRFTYE
                     DFLLNKDLTPVFPTWLRSGNVGTGDPCKMAFPDPYTFSITFSQPYGAFPTMLADTLGW
                     RGYTELIKPKHYLKNYHTKYTALSKMAAEIQKDGFKEGEWWQLFNKKDCTNWEVFTED
                     AIGFPSLSPWIAKTITQQTIIMERNPYFWKVDTAGNQLPYIDTLRSDLLSDVKNVTMK
                     IISGEVDHSYEFGTLPQLPLYKENQAKAGYNMNFYLMHRTEADFLLNFTYPDPVWKKV
                     VSDVRFRQALNKAVKYSDIVENVYFGFAKVPYTVPGSYDPAGANKLLDEMGLNKKDAD
                     GFRLGPDGKRFTIPIEIQAYFDTIPKCAEIVAENWKAVGLYTTVKVNEASLQNSRQSA
                     NQVAVYFQWPLRADVWWAVWITPWDSSPLWQLWFNTGGKQGEEPPAAMKECYDLLQKM
                     YVVSPAEREKLHTQFLKNVADNLWYGITAEDIQYLVMTNKNMGNVAKQGFGIASQFSG
                     EQFFFKQ"
     gene            7342..>8556
                     /locus_tag="VFH83_05415"
     CDS             7342..>8556
                     /locus_tag="VFH83_05415"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET6485834.1"
                     /translation="MDTSFDLKVKGSLAGVVIGTELGMQRLLIGEPRVSAFDAVKALA
                     ASLPWRAAPEQTPKRHSFCALTPLMASVARTYLKRDGRITAEDWGSELAQDPALSENP
                     AFWLLDVHTTIEILREGMNARVSGLYAMPCGDACAAAVPVGIYHAGDADAAYVDGVEV
                     ASVMQRAPGSDWAALTACAVAEALKPDATAQSVVDRTLRVALSRARDVYYELNHLVQS
                     ARSFFRPPAKPEWSREAFCTFFAQANASERHGWLGHNPIGWALALLSAFPEEPEALVK
                     ASVILEAYPSIRSPIVGALVGALWGQRAFPEDWWSHAAAGIEPLAAGMVRLAKKRLEA
                     ETAIITQIEELARPRNGADSLLFDKVYGCILAGAIGNAMGSVVEGKHWDEIDREHPQG
                     ILTVLDPSRLEGE"
BASE COUNT         1657 a         2723 c         2774 g         1402 t
ORIGIN      
        1 acgcgggaag gtgggggagc acggccgcgt agcccggtag aagcggtctc aggtccgccc
       61 tcaggaacac ctccaggatc gcgccccttc gcgtcttcgg gtttctcggc acccgccgtg
      121 cgtagtacgc gagggctctg agcatcgagc cgtcatcccc gggggagagg aggctcaccg
      181 atgaggagcc ccggtattcc ggaatctcct tctcggtgtt gtcgcggtac cgcagtccaa
      241 acccggcgcg ctccaggacc cgcgtggccc aacgcacgtc ccggacaccc gcctgacgac
      301 gacgggcttc caggtctgcc gtgtccgcgc gcagcgtgga cccccaggga ccgcgggtct
      361 gctcgccgca ggttcccagc gcccacagca tccggaccgg cgcctcgatg gcgcggaagc
      421 agaactcccg atccagccac cgctcgcctc gcggggccat cgcgggagcc aacgcataca
      481 ccgcgccgat ccgctctcgg agccgccgca gtcccccaaa ggtcgcggca cgctctcggg
      541 gtgggacctc cagatgctgg gattgcagtt cctccgggaa gtcaaccaga aagaggcgat
      601 aggatgcggc gaggtcatgg atgggagagg ccaacgtttt catgggacgc tcctgcgtcg
      661 acggaacgtt tcatccgcgg ttccgcggac accccacggg gaatcatacc agaagcgggg
      721 cgccggtgga aggaaaaaag tttacttccc ggcccggagc ctgtataatg gcgccgcttc
      781 gttgtcaggc gaaggagggt tgaatgtcgg atagctcggg cccgctcctg gaggttgccg
      841 gcctcaagaa gcacttcccc atcaagaaag gcttcctcaa gcggaccgtc ggctacgtgc
      901 gagcggtcaa cggcatcgac ttctacatcc ggaggggcga gacgctgggc ctggtggggg
      961 agagcgggtg cgggaagacg acgacgggcc gcatgatcgt ccgagccctc aagcccaccg
     1021 cgggccagat tctcttccat accaacggga cgacgaccga cctggctccc ctggagttcc
     1081 gggagctcaa gcccttcaag aagcacatcc agatgatctt ccaggacccg aactcgtcgc
     1141 tcaacccgag gatgacgatc ttggagatca tcggcgagcc gctcatcgtc aacggcgtgg
     1201 cgcgcggcca ggagctcgag gaccgggtcc gggagctgct gaagaccgtc gggctgcgcg
     1261 cgcagttcct gcgccgctac ccccacgcgt tcagcggggg ccagcggcag cggattggaa
     1321 tagcccgcgc gctggcgctg aacccggacc tcatcgtcgc agacgagccc gtctcggcgc
     1381 tggacgtttc catccaggcg cagatcctga acctgctcca ggacctccag gaggagttca
     1441 ggctcagcta cctcttcatt gcccacgacc tgagcgtagt ggagcacatc agcaaccggg
     1501 tggccgtcat gtacgtcggc cagctcgtcg agatggcttc tacggacgag ctcttcccgc
     1561 ggccccgcca tccatatacc gaggcgctgc tgtccgcggt acctgttccc gatcccatca
     1621 acaagcgcaa gcgcctgatc cttcccggag aggttgcgga tcccgccaat cctccgccgg
     1681 ggtgcatctt ccatccccgg tgcaagtacg ctcgggagat ctgcagcaat gaggtgcccc
     1741 atctggagga gatcgagcag gagcacttcg tgtcctgcca cctggcgcgc gagctttccc
     1801 tcaaaggggt gaagaaatga gctctccggc aacggtcgcg cagaacacgc tcatcgaggt
     1861 ccagaacctc aagacgcact tcgttctgga cgagggactc ctcaaagcgg tggacggcgt
     1921 cagcctgtcc atcgagcggg gcaagaccct gggcctggtc ggggagtccg gatgcggcaa
     1981 gagcgtgacg gcgcagtcca tcatgcggat cgtgccgcct ccggggagga tcgtgcaggg
     2041 gaagatcctc tacagccggc cgggcgatgg cggttccgca gggacggtgg acctggcctc
     2101 tctggaggcg tccggtaccg acatgcggag gatccggggg aaagacatca cgatgatctt
     2161 ccaggagccc atgacctcgt tctcaccctt gcacaccatc ggcaaccaga tcatggaggc
     2221 catcctcctg catcggagcc gggacccggc cgaggcgcgg gagatcgcta cggacatgct
     2281 ggccaaggtg gggattccca acccaggcca gaggatcgat cagtaccccc acgagctctc
     2341 cggcgggatg cgccagagag ccatgatcgc catggcgctc tcctgcaatc cgtccctgct
     2401 catcgcggac gagccgacca ccgccctgga cgtaaccgtg caggcccagg tcatcgagct
     2461 catggagggg cttcgccagg agctggggat gtccatgctc tacatcaccc atgacctggg
     2521 ggtcatcgcc gaggtggcgg acgaggtcgc cgtcatgtac ctgggtcgga tcatcgagcg
     2581 gggaagcgtc atggacatct tctaccacgc ggcgcacccc tacacgcggg ccctgctgca
     2641 atccgtcccg cggatgggca gacgcggaca acgccgtcgt ctgcaggcca tccggggcac
     2701 ggtccccatc cccatcaatc tggtggatcg ctgcgcgttc cgcacccgat gccagcactt
     2761 cgagcgatcc aaatgcatgg gatccgagcc ggccctggtg gagatcgaga aggggcactg
     2821 ggtgcgctgc gtgcgttacg acgagcttgg ggaaggggac ggggctctgc cctgagctcc
     2881 ctgccgttca gcgcgcaaag gggtccgccg agtcccgaag cccatctccc aggaagttga
     2941 acgccaggac cgcgatcacc acgaagatgc agggggtcaa caaccagggg tagtgcacca
     3001 gggccgagag gtcctgcgcg tcctgcagaa gcgtgcccca gctcacggcg ggcggctgca
     3061 tgcccagacc gatgaaggag agcgccgtct ctcccaggat catgttgggg acgctgagcg
     3121 tcagggacac gatgatgtag ctcgcaaaag acgggatcag gtgacggccg atgatccgca
     3181 ggttgctcga gcccgccacc tgggcggcga tgacgaagtc ctcctcccgc agcgacagga
     3241 gcttgcctcg caccacgcgg gccagaccgg tccagccgat gatcgagaag atgaccgtga
     3301 tgccgaagta gatgcggacc acgggccaat ctcgcgggag cgccgcggcc agtgccatcc
     3361 acaacgggat ggtggggatg gagatcagca ggtcgatcga ccgctggatg atggtgtcgg
     3421 taaccccgcc gaagaacccc gacagtcccc ccagaagcag cccgaagatg aaggtgaacg
     3481 tgattcccac cagaccgatg gagagggaga tccgggtgcc gtacaggatc cgggtgaaca
     3541 cgtcccgccc cagacggtcc gtgcccagca ggaagatcgg aaacccgtcc gaggtcacca
     3601 ggtggacgtc ggtgcgaaag aggccccaga acttgtagcg ctcgccgtgc gcaaagaact
     3661 tcaccggata cttggtggcc gggtcaatca cgaagatccg ccgatacgtg gcgggatcga
     3721 cggtcttgtt gtagccgtag atgtagggcc gcaggaatcc gttgccgatg agacgcaccc
     3781 ggttgggcgg cgcgtagaca tagtcgctgt agcgggtgag gggcgtgtag gggctcagga
     3841 actccgcgaa tacgacgatg aggtacagca ggatcagggc caccgcggcg cccatggcca
     3901 gcttgtgcct gcgcagcttc caccagatca gcttccactg cgaggcgacg tagtactcct
     3961 cctcctcacg gtcctcctgc tccagctctt cgagctggtc ctcggtaagc cggctcttct
     4021 ccctcattcc ccgattcctc cgtagcggat gcgcggatcg agcacggcca gcaggacgtc
     4081 cgagatgagc gtgccgatga cggtcatgac gctcaggatg agcaggaagc tgccggccag
     4141 gtacatgtca cggtccagaa gcgcgcgcag aagcatggga cccgtggtcg ggatgttgag
     4201 cacgatggcc accaggggcg tgttcgagat cagggccgga aggagccatc ctacggtgct
     4261 cacgatcggg ttgatggcga cgcggacggg gtacttgaag aggagcgtcc gctcatccac
     4321 gcctttggct cgggcggtga tcacgtattg cttgcgaagc tcatccagaa ggcaggcgcg
     4381 catcacacgg atggtgtacg ccgtgccgga caaccccacg atgagcacgg ggatccagat
     4441 ccgcttcgcc atgtcgataa acttggcaag gctccacggg gcgtcgacgt acgccgggga
     4501 gaagagcccg gtcaccgaga tgccaaggta tcggaacgag aagtagacca gggccagcgc
     4561 cagcaggaac ggggggacgg agaggcccac gaaccccagg aaggtgaagg tgtagtctcc
     4621 gatcttgtat tggtgcgtgg ccgagtaaat gcctatcggg atggctaacg cataggtgac
     4681 gaggagcgag aggatggaga gcaggatggt gagcccgatc tgctcgccga tgaggtccgt
     4741 caccggcttg ttccactcga acgaccttcc caggctgccg tagcggacca tgttccacat
     4801 ccacagcagg taccgctcgg gcagggactg gtccaggcca tactgacggg tcagtcgcgc
     4861 gatctcatcc gcgccgggct gagttcccga ggtcttgagc tggttgatgt agatggacag
     4921 gtagtcgccc ggcgggagaa tgatgctgat gaagatgagg accgaaagca ccaggagcag
     4981 cggaatgagc tgtagaatcc gcctgccgat gtagctgagc atgagagctc ccttcggtgg
     5041 atctcggggc gacgccgtcg atagcgccgg gcgatcagtc ccggcatctt actcgacgca
     5101 aattccactg tcaagaaaaa tcccgggcgg gcggcgcgcc gtcgccgcac agcctgccgg
     5161 cgccgggttg agcgggcgaa aacagaaaaa gcttgacttc ccgcattgcc tgtcctacaa
     5221 tcgacaccct cgggtctgac cttagcaatc gaaacaaggt cggaggcgcg ttgcgtttgg
     5281 atgccgtttc cttgcccgag gtcgacccct tggctctctt ctggatcgtt gatccaggtt
     5341 gatcgcatca aggaggtgga atcatggcaa aaagggtcct gctagccttg ctggcgctcg
     5401 ccatcctctt gccagcggcc ttcgcccagg ctcccggaaa gtatcctatc ccggcgacat
     5461 tcaagcagtc gccgatgctg gacgacctgg tgaagtctgg caagcttccc ccggtcaagg
     5521 accggcttcc caaacatccc ctggttgtcg gactgggcac ggacatgagt gccacggacc
     5581 tccccgactg gcaggtcgga aagtacggag gcacggagcg catgccgcgc atctggccta
     5641 cggcggacta tggcatgttc atccaggaca accagccgat catccgcggg gtggggctgg
     5701 cgcaggagcc gttctacggc aacgtcgtgg agtcctacga cgtctccgcg gaccagaaga
     5761 ccttcacctt ccacatgcgg gacggcatga agtggtccga cggacagccc gtcacctcgg
     5821 atgacgtgcg gttcacgtac gaagacttcc tgctcaacaa ggacctgacg ccggtcttcc
     5881 ccacctggct gcggtccggc aacgtcggga ccggggatcc ctgcaagatg gcgttccccg
     5941 acccgtacac cttcagcatc accttcagcc agccttacgg cgcgttcccc accatgctcg
     6001 cggacacgct gggctggcgc ggctacaccg agctgatcaa gcccaagcac tacctcaaga
     6061 actaccacac caagtacacg gcgctctcca agatggcggc ggaaatccag aaggacggct
     6121 tcaaggaggg agagtggtgg cagctcttca acaagaagga ctgcacgaac tgggaagtct
     6181 tcacggagga cgccatagga ttccccagcc tttccccgtg gatcgccaag acgatcaccc
     6241 agcagacgat catcatggag cgcaacccgt acttctggaa ggtcgatacc gcgggcaacc
     6301 agctccctta catcgacacg ctgcgctcgg atcttctctc ggacgtgaag aacgtcacga
     6361 tgaagatcat ctcgggtgag gtggaccact cctacgagtt cggtacgctg ccccagcttc
     6421 cgttgtacaa agagaatcag gccaaggccg gctacaacat gaacttctac ctcatgcacc
     6481 gcacggaggc agacttcctg ctgaacttca cctatccgga tccggtctgg aagaaggtcg
     6541 tgagcgatgt ccgcttccgc caggccctca acaaggcggt gaagtactcg gacatcgtcg
     6601 agaacgtgta cttcggcttc gccaaggtcc cctacacggt gccaggctcg tatgaccctg
     6661 cgggcgccaa caagctcctg gacgagatgg gcctgaacaa gaaggacgcc gatggattcc
     6721 ggctgggacc ggacggcaag cgcttcacca tccccatcga gatccaggcc tacttcgaca
     6781 cgatccccaa gtgcgccgag atcgtggcgg agaactggaa agccgtaggc ctgtacacca
     6841 cggtgaaggt gaacgaggcc tccctgcaga actcgcggca gagcgcgaac caggtggccg
     6901 tgtacttcca gtggcccctg cgcgccgacg tctggtgggc ggtgtggatc accccgtggg
     6961 actccagccc gctctggcag ctctggttca acacgggcgg caagcagggt gaggagccgc
     7021 cggctgccat gaaggagtgc tacgacctcc tgcagaagat gtacgtggtc tcccccgccg
     7081 agcgcgagaa gctgcacacc cagttcctca agaacgtggc ggacaacctc tggtacggga
     7141 tcaccgcgga ggacatccag tacctggtca tgaccaacaa gaacatgggc aacgtggcca
     7201 agcagggctt cgggattgcc tcccagttct cgggggagca gttcttcttc aagcagtagg
     7261 acgatcgatt ctcatgagat gacggtgccg ggtcgtctga cgacccggca ctttttttgg
     7321 cccgtttcgc agggggaaac catggacaca tcattcgatt tgaaggtgaa gggtagtctg
     7381 gccggagtcg tgatcggcac cgagctcggc atgcagcgtc ttctcattgg ggagcctcgg
     7441 gtctcggcct tcgacgccgt caaggccctc gccgcctcgc ttccgtggcg cgccgcgccg
     7501 gagcagaccc ccaagcgcca ctcgttctgc gcgctcacgc ccctcatggc gtcggtcgcg
     7561 cggacgtacc tcaagcggga cggccgcatc accgcggagg actggggaag tgagctggcc
     7621 caggaccccg ccctctccga gaacccggcc ttctggctcc tggacgtaca caccaccatc
     7681 gagatcctgc gggaagggat gaacgcccgc gtgagcggtc tgtatgcgat gccctgcggg
     7741 gatgcgtgcg ccgcggccgt gccggtcggt atctaccatg ccggggacgc ggacgcagcc
     7801 tacgtcgacg gggtggaagt ggcttccgtg atgcagcgag cgcccggctc ggactgggcg
     7861 gcgctcaccg cctgcgccgt agcggaagcg ctcaagccgg acgccaccgc tcagtccgtg
     7921 gtggaccgca cgctgcgggt ggccctgtcc cgcgcccggg acgtctacta cgagctcaac
     7981 cacctggtgc agtccgcgcg gagcttcttc cgcccccccg ccaaaccgga gtggtcccgg
     8041 gaggcattct gcacgttctt cgcccaggcc aacgcgtccg agagacacgg ctggctgggg
     8101 cacaatccga tcggctgggc gctggctctg ctgagcgcct tccccgagga gcccgaggca
     8161 ctggtcaagg cttccgtcat cctcgaggca tacccgtcca tccgctcgcc gatcgtcggc
     8221 gccctggtcg gggcgctctg gggacagagg gcctttccgg aggactggtg gtcgcacgcg
     8281 gcagccggca tcgagccgct tgcggcgggc atggtcaggc tcgcgaagaa gcggctggag
     8341 gcagagaccg ccatcatcac ccagatcgag gagctggcac gacctcgcaa cggggcggac
     8401 agtctcctgt tcgacaaggt ctacgggtgc atcctggccg gcgccatcgg caacgcgatg
     8461 gggtccgtcg tggaaggcaa gcactgggat gagatcgacc gggagcatcc ccaagggatt
     8521 ctcactgtcc tggaccccag ccgtctggaa ggcgag
//
LOCUS       DASRZS010000215         5606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2815786, whole genome shotgun sequence.
ACCESSION   DASRZS010000215 DASRZS010000000
VERSION     DASRZS010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2815786"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFH83_05420"
     CDS             <1..315
                     /locus_tag="VFH83_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485835.1"
                     /translation="LQESGAYLEECRRQGLYSPVANERIERVLEICQVFLGETDESST
                     GRRYGIAEGSSEADAGSPSERFSRDIVECAGVIKQRTTEDLLGQSGAERLSILKELEE
                     KK"
     gene            312..1964
                     /locus_tag="VFH83_05425"
     CDS             312..1964
                     /locus_tag="VFH83_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485836.1"
                     /translation="MSRARSMVLVAAVFLAVVAPGGFALSADLKSVETDIVVRTDGKA
                     TFYESLDWSASGGQMHGFYFQGAAVTPVFDLSQSFADLTGKVRVPLAITDLGGGKYDV
                     VLAGGRGFSGSAMYYLTYGGDLSGPGLLTWSKSDQFGQLFDFDWAAVQWDEPLEHRTT
                     RIVLPVVVAGQTVTQDDLTKLGFRTEPYVNNENSIDSYGTKGADGKYYLTLRFHQENV
                     PTRGAQRLQFYLTLAAVPMQSGVLSEAASGAAGASGSSSQNAAPAEQPAPEVVRTPLS
                     LAVAVPILAVLLVLVLLLYWLKTRGYGKTLEKVEGIRWAGDNWIPPKLFSGTYQVKGK
                     IIKDLHPIEAALLLEMPLQRVVGIMLEGLKRQGIIQVVTEDPLQIKILTARKAEHEYE
                     ELFLQSFDTKGLVLSGLLADFFEKVLARLQEKIWDCDIDATKEFYRKKLEQKEETEED
                     RRDPHYYYWSSYRWMFWNAAVYSTIRLPHDFTAGYQTFMASASCFKGCFAPPQTQTGG
                     VGACYSACHNACHSACHHACHSACHSACHSACHSACVSGGAH"
     gene            1961..3388
                     /locus_tag="VFH83_05430"
     CDS             1961..3388
                     /locus_tag="VFH83_05430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6485837.1"
                     /translation="MRRLGGGPRAALRSLAWIDEFFRKARGHIYAREEDGVVILPPNQ
                     VYKANATGISILRHFLRGGRVRGIPGIEEADRARDVAGFVSDLRSRYLGEPAPAGDTV
                     PYDFQYTTLPILGEIAVTYRCNNACLFCYAGCGAGSCAPPPGSTARSPAATPSELAGG
                     REMSLSECRRIIRLFKEEAKIPFFSFTGGEPLLRPDLEEMIRYAVGIGLQVNLITNAT
                     LADAPRAASLYRAGLRTAQVSVESPDEERHDHLTARPGSWKRTLAGLACLQAAGVSVQ
                     TNTTVTAANAEDTPRMPAFLKGLGVSRFAMNLYLPPGASAEEAGPHAAGLFVPYTRAG
                     SIIEAVRAAARAHGLTFYWYSPIPHCHYNTIAKGLGNKSCAAMDGLLSVSSAGDVLPC
                     SSYPQPMGNLLRQGFPEIWFSSRAQFFKQKRYAPTECGGCESFTACQAACPLYWRYAG
                     TSEIRNPAAGVTAGARTAAPAPAAS"
     gene            3407..4303
                     /locus_tag="VFH83_05435"
     CDS             3407..4303
                     /locus_tag="VFH83_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485838.1"
                     /translation="MEMKYVIPKVSFPARLLALAGFGAAGMALQVLVPNGFGFLPGAV
                     VMVPGVLFLIARNFRNKPMDIGQEDWQPASVLEFDRIKRNLQMTRQKRYAVIYRPGFG
                     IFILIVLLAFALILSAGGRAFGATVFFDAALLLIGFLLSGNVSLWTPQELAFRMAVFE
                     PILSTEQAEGGDIIITPYLRLDKDREGHQVPEDIRLMVEPRRKPADFLGVQLQVAVNK
                     GPNGNVPYLYSVFLCRGKGATYQALAADHYGAFVREPGGDKEYGYVVVRQQTSGTGYQ
                     TDASDVRRLYAMVKEKLLKLQR"
     gene            complement(4321..4701)
                     /locus_tag="VFH83_05440"
     CDS             complement(4321..4701)
                     /locus_tag="VFH83_05440"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HET6485839.1"
                     /translation="MAYIEWTDEFVVDNELINLQHKTLIGIVNSLHDAVTHGEDSDIV
                     DSSLSGLIDYTHFHFKAEEDLLTGSAELDLQKKEHDRMARQVMALNEKYHQGSATMIF
                     EILDLLHDWLSNHVKRLDKRSFSG"
     gene            complement(4712..5125)
                     /locus_tag="VFH83_05445"
     CDS             complement(4712..5125)
                     /locus_tag="VFH83_05445"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HET6485840.1"
                     /translation="MAFIQWSDQLSVSVRTIDEQHKRLIGIVNDIHATVVAGAERTAL
                     NRIFDELISYTVYHFRTEEEFFEKYRYTDRELHKSQHNELTQKALELQETYQQGSATV
                     SYEVLDFLHDWLVTHIIGSDRQFGKFLAARGAAAV"
     gene            complement(5150..>5606)
                     /locus_tag="VFH83_05450"
     CDS             complement(5150..>5606)
                     /locus_tag="VFH83_05450"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6485841.1"
                     /translation="ALGTIGSDDRLNATVIGDPVNLASRVETLTKVFGADLILTDSIS
                     RQLARPGRYNLREIDTVRVKGKQTPIVLYEAFDTNPPELVEKKKMMMGDYERALSLYK
                     QGSFQPAMDLFTACKDFCPEDKIPDIYIRRCNSLIRIPPGKDWAGISTL"
BASE COUNT          929 a         1835 c         1849 g          993 t
ORIGIN      
        1 ctgcaggagt ccggcgccta cctggaggag tgccgccgcc agggattgta ctcccccgtc
       61 gccaacgagc gcatcgagcg cgtcctggag atctgccagg tgttcctggg ggaaaccgat
      121 gagagctcca cgggtcgccg ctacggcatt gcggagggaa gctcggaggc cgacgccgga
      181 tctccctcgg agcggttctc gcgagatatc gtggaatgcg ccggtgtcat caagcagcgg
      241 accacggaag acctgctggg gcagagcgga gcggagcggc tttccatcct gaaggaactg
      301 gaagagaaga aatgagtaga gcgcgttcga tggtcctggt tgccgcggtg ttcctcgccg
      361 tggtggcgcc cggcggtttc gccctgtccg cggacctgaa gtccgtggag acggacatcg
      421 tggtgcggac ggacggcaag gcgacgttct acgagagcct ggactggagc gcctccggcg
      481 gccagatgca cggcttctac ttccagggag ccgcggtcac accggtgttc gatctcagcc
      541 agagcttcgc ggacctcacc ggcaaggtcc gggtcccgct ggccatcacc gacctgggtg
      601 gcggcaagta cgacgtggtc ctggcgggcg gtcgcgggtt ctcgggcagc gcgatgtact
      661 acctcaccta tggtggagac ctgtccggcc ccgggctcct cacgtggagc aagtcggacc
      721 agttcggcca gctcttcgat ttcgactggg cggcggtcca gtgggatgag cctctggagc
      781 accggaccac gcggatcgtg ctgccggtcg tggtagccgg ccagaccgtg acccaggacg
      841 atctcaccaa gctgggcttt cggacggaac cctacgtaaa caacgagaac agcatcgatt
      901 cctacggcac caagggcgcc gacggcaagt actacctcac cctccggttc caccaggaga
      961 acgtgcccac tcggggcgcg cagcgcctgc agttctacct gaccctcgcg gcggttccga
     1021 tgcagagcgg cgtgctttcc gaagccgctt cgggcgcggc gggcgcctca ggctcctcca
     1081 gccagaacgc ggcgccggcg gagcagcccg ctcccgaggt ggtccgtacg ccgctgagcc
     1141 tggcggtggc cgtccccatc ctggcggtcc ttctggtcct ggtcctcctt ttgtactggc
     1201 tgaagacccg cggctacggc aagaccctgg agaaggtgga aggaatccgg tgggccgggg
     1261 acaactggat cccgcccaag cttttctccg gcacctacca ggtcaagggc aagatcatca
     1321 aagacctgca cccgatcgag gcggcgcttc tcctggagat gccgctgcag cgcgtggtgg
     1381 gcatcatgct ggaaggtctg aagcgccagg gcatcatcca ggtggttacg gaggacccgc
     1441 tccagatcaa gatcctgacg gcgcgcaagg cggagcacga gtacgaggag ctcttcctcc
     1501 agagcttcga caccaagggg ctggtgctct ccgggctcct ggcggacttc ttcgagaagg
     1561 tgctggccag gctccaggaa aagatctggg actgcgacat cgacgcgacc aaggagttct
     1621 accgcaagaa gcttgagcag aaggaagaga ccgaggagga ccggcgcgat ccgcactact
     1681 actactggtc atcctaccgg tggatgttct ggaacgccgc cgtgtactcg accatccgcc
     1741 tgcctcacga tttcaccgcg ggctaccaga cgttcatggc ctccgcgtcc tgtttcaagg
     1801 gatgcttcgc gccgccccag acccagaccg gcggtgtggg cgcctgctac tccgcctgcc
     1861 acaacgcgtg ccactcggcc tgtcaccacg cctgccattc ggcgtgtcac tccgcctgcc
     1921 actcggcatg ccactccgcc tgtgtatccg gaggcgcgca ttgagacgac tcggcggcgg
     1981 gccgcgtgcc gcgctgcgct ctctggcctg gatcgacgag ttcttccgca aggcgcgagg
     2041 ccacatctac gcccgggaag aggacggcgt ggtgatcctg ccgcccaacc aggtctacaa
     2101 ggcgaacgcc accggcatct cgatccttcg gcattttctg cggggaggcc gcgttcgagg
     2161 gatcccgggg atcgaggagg ccgaccgcgc gcgggacgtg gctggattcg tgagcgacct
     2221 gaggtcccgc tacctggggg aacccgcccc agccggtgac acggtgccgt acgacttcca
     2281 gtacaccacg ctgcccatcc tgggtgagat cgccgtgacc tatcggtgca acaacgcctg
     2341 cctcttctgc tacgcgggct gcggggccgg ctcctgtgct ccgccgccgg gatcgacggc
     2401 gcgctcgccc gccgcgacac ccagcgagct ggcgggcggc cgggagatga gcctgtcgga
     2461 atgccggagg atcatccgcc tcttcaagga agaggcgaag atccccttct tctccttcac
     2521 cggcggcgag ccccttctgc ggccggacct ggaagagatg atacgctacg cggtcgggat
     2581 cggcctgcaa gtgaacctga tcaccaacgc gacgctggcc gacgcgccgc gcgccgcctc
     2641 gctgtaccgg gcgggtctga ggacggcgca ggtcagcgtc gagagtccgg acgaggagcg
     2701 gcacgaccac ctcacggcgc gcccgggctc ctggaagcgc acgcttgccg ggcttgcctg
     2761 cctccaggcg gcgggcgttt cggtccagac caacaccacg gtgacggctg ccaacgcgga
     2821 ggacacgccg cggatgccgg ccttcctgaa ggggcttggc gtgagccgct tcgccatgaa
     2881 cctctacctt ccgccggggg ccagcgccga agaggccggg cctcacgccg ccgggctctt
     2941 cgtcccgtac acccgagccg ggtcaatcat cgaggcggtc cgggcggcgg cccgagcgca
     3001 cgggctgacg ttctactggt actcccccat cccgcactgc cactacaaca ccatcgccaa
     3061 ggggctggga aacaagtcgt gcgccgcgat ggacgggctc ctgtccgttt cctcggccgg
     3121 agacgtcctt ccctgctcct cctacccgca gcccatgggg aacctgcttc ggcagggctt
     3181 cccggagatc tggttttcgt cccgagcgca gttcttcaaa cagaagcgat acgcgcccac
     3241 ggagtgcggc ggctgtgaaa gcttcaccgc ctgccaggcc gcctgcccct tgtactggcg
     3301 ctacgcgggc acctcggaga tccgcaatcc tgcggcgggg gtgacggccg gggcgcggac
     3361 cgccgcgcct gcccctgccg cgagctgagg gaatggagat cgagccatgg agatgaagta
     3421 cgtaatcccg aaggtgagct tcccggcgcg tctcctggcg ctggctggct tcggcgccgc
     3481 gggcatggcg ctgcaggtgc tcgtgcctaa cggcttcggg tttctgcctg gggcggtggt
     3541 catggttccg ggggtcctct tcctgatcgc ccgaaacttc cgcaacaagc ccatggacat
     3601 cggccaggag gactggcagc ccgcctcggt gctggagttc gaccggatca agcggaacct
     3661 ccagatgacc cgacagaagc gctacgccgt catctatcgc cccgggttcg ggatcttcat
     3721 cctgatcgtg ctcctcgcct tcgccttgat cctctccgcg ggaggaaggg ccttcggggc
     3781 aacggtcttc ttcgacgcgg cgctgctcct catcgggttc ctcctgagcg gaaacgtgtc
     3841 cctctggacg ccccaggagc tggcgtttcg catggcggtg ttcgagccga tcctgtcgac
     3901 ggagcaggca gagggcggag acatcatcat cacgccgtac ctcaggctgg acaaagatcg
     3961 ggaaggccac caggtccccg aggacatccg gctcatggtg gagccccgac gaaagcccgc
     4021 ggacttcctg ggcgtgcagc tccaggtggc ggtcaacaag ggccccaacg ggaacgtgcc
     4081 gtacctgtac tcggttttcc tctgccgcgg gaagggagcc acctatcagg ccctggcggc
     4141 ggaccactat ggcgctttcg tgcgagagcc gggcggggac aaggagtacg ggtacgtcgt
     4201 ggtccgccag cagacctccg gtaccgggta tcagacggat gcctcggacg tccgccgact
     4261 ctacgccatg gtcaaggaga agctgctgaa gctgcagcgg taagcgcccg cgcccaccct
     4321 tcagccggag aacgacctct tgtccagccg cttcacgtgg ttactgagcc agtcgtggag
     4381 caggtccagg atctcgaaga tcatggtcgc gctcccctgg tggtacttct cgttcagtgc
     4441 catcacctgg cgagccatgc ggtcatgttc cttcttctgc aggtcgagct ccgcgctgcc
     4501 ggtcaggagg tcttcctcgg ccttgaagtg gaagtgggta tagtcgatca ggccggagag
     4561 ggacgagtca acgatatcgg agtcctctcc atgcgtcacg gcgtcgtgca gggagttgac
     4621 gatgccgatg agcgtcttgt gctgcaggtt gatgagctcg ttatccacta cgaactcgtc
     4681 ggtccactcg atgtacgcca tggtcggtcc cctacacggc cgccgcgccg cgcgccgcca
     4741 ggaacttccc gaactgccga tcggacccga tgatatgggt caccagccag tcgtggagga
     4801 agtccaggac ttcgtaggat acggtggcgc tgccctgctg ataggtctcc tgcagctcca
     4861 gcgccttctg ggtcagctcg ttgtgctggg acttgtgcag ctcgcgatcc gtgtagcggt
     4921 acttctcgaa gaactcctcc tccgtgcgga aatgatagac ggtgtagctg atcagctcgt
     4981 cgaagatccg gttcagcgcg gtccgctccg cgccggccac cacggtggcg tggatgtcgt
     5041 tgacgatgcc gatcagccgc ttgtgctgct catcgatggt gcgcacggag acgctgagct
     5101 ggtcggacca ctggatgaac gccatgactg ccgctccttt gctccggcct tacagcgtgc
     5161 tgatgccggc ccagtccttg ccgggcggga tgcggatcag cgagttgcag cgccggatat
     5221 agatgtccgg gatcttgtcc tcgggacaga agtccttgca cgccgtgaag aggtccatcg
     5281 cgggctgaaa cgagccctgc ttgtagaggg acagggcacg ctcgtagtcc cccatcatca
     5341 tcttcttttt ctcgaccagc tcgggagggt tggtgtcgaa cgcctcgtag agcacgatcg
     5401 gggtctgctt ccccttcacg cgcaccgtgt cgatctcccg caggttgtac cgtcccggcc
     5461 gggccagctg acgggagatg gaatccgtca ggatcagatc cgctccgaac accttggtga
     5521 gcgtctccac ccgggaggcc aggttcaccg ggtccccgat gacggtggcg ttgagccgat
     5581 cgtcggaacc gatcgtgccc agggcg
//
LOCUS       DASRZS010000216         6398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2430177, whole genome shotgun sequence.
ACCESSION   DASRZS010000216 DASRZS010000000
VERSION     DASRZS010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2430177"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VFH83_05455"
     CDS             <1..1044
                     /locus_tag="VFH83_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET6485842.1"
                     /translation="RLARSRAFSSLEEAFSTVLDSSEGAGLGIVILVLMMKKIGLSED
                     CFSIDAEDGETVASITIPFSEVHMEQLDALTQLLVKEVETLPQFPDNIVNLQRLIADP
                     NAEITDIAREISVDPSMTADLLKLVNSAQFMLPKRVDNIVEAVKLVGIKGVRNLLYSY
                     GTQKVLGEKYSEMRSLWQDSYRCAFYAFSLARGFKRRKEILDDVYVGGILHDLGQIVV
                     ASLHPDLLERITRVCREKGISSELLEDFSVGLNHAEVGALIARKWNFPEQLVGAIRYH
                     HEPLECPAGLREIVYPVYLAAAICDIERERIVFNQIHPHVLQDFNIETPEQLEKIQTQ
                     LAKAFEAQRTKFQ"
     gene            1157..1387
                     /locus_tag="VFH83_05460"
     CDS             1157..1387
                     /locus_tag="VFH83_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxx-star family glutaredoxin-like
                     (seleno)protein"
                     /protein_id="HET6485843.1"
                     /translation="MAITIYTTPTCSYCKVAKEFFRTQHVPFTEYNVASDQRKADEMV
                     RKSGQMGVPVIEINGKVIVGFNRPEIEKALRC"
     gene            1439..1786
                     /locus_tag="VFH83_05465"
     CDS             1439..1786
                     /locus_tag="VFH83_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485844.1"
                     /translation="MYKAILGTVTDVRPELLDQRLRVVAAVRTQQGELLQAHMPDREV
                     AAVLPRSVLLGAARTAPPTLLDTAQPILARMTVGRLVRLWRYQERWFFSFQGWKGVRF
                     VEESTQPAMAGPG"
     gene            complement(1767..2555)
                     /locus_tag="VFH83_05470"
     CDS             complement(1767..2555)
                     /locus_tag="VFH83_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HET6485845.1"
                     /translation="MFPLTKLGELPPRTRLRKISRILQSLEVGLMGGVPPDPAYVARL
                     AELANDAASRRMGSPLLPSGLASLPPAALLRALNQARHGILRELGSEPAEWDLVSPES
                     GRLDRSQVAVLPLAVYLEDLRSPYNVGSIFRTAEAFGVQRILLSPRTPLPTHPRAERT
                     ARGAGQAIPWETADLDRLRGEAGIFALEVGGTPMERFAFPNRGLVLVGSEELGLSPEA
                     LSLADAGGGRVTIPMAGAKRSLNVAVAFGILMHAWYVCVTQGRP"
     gene            complement(2563..3711)
                     /locus_tag="VFH83_05475"
     CDS             complement(2563..3711)
                     /locus_tag="VFH83_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET6485846.1"
                     /translation="MPDVLFHVPTRVVFGLDTVNRIGQIASELGARALLVTEAILYEG
                     KVINKVQDILDKKGLPYITFDEVVPNATSRTVDDGVRLARGSHAEVIIGLGGIRTLSI
                     AKSIAMTAPAPQDIDEYLSGVQPSERPLAYVEIPTTCRDPFLFVDEYLMVDGRDRSPR
                     IGRTPHGITSAALIDPKLSLTLPAKYTATTILDTLLGCVEGYLSSRATFLSDTYLTRA
                     IEILGGIIREGLQNVDDIRIRMSASMAGLLQALGLSTSKLGIGSALAFAINARYMAPK
                     SWVSTIMLPFAMQFAVNVRGDRLVEVGRLLRLDVGEGSPAEAPLRTIEAVRRIVSTLS
                     LPTRLREFDFSLDEMVEVAGATRSFDMMNYLPRVVSSEDIYELIKSAY"
     gene            complement(3787..4071)
                     /locus_tag="VFH83_05480"
     CDS             complement(3787..4071)
                     /locus_tag="VFH83_05480"
                     /inference="COORDINATES: protein motif:HMM:NF016223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HET6485847.1"
                     /translation="MTVERIGPLDPASNLPKSDRPAKTKAKSDVDSINVSQEARSKAE
                     VFNAMEAAKGSPDVRVDRINEIKRKLQDPSYPSQEVIEKTAEGIMKSFGV"
     gene            complement(4160..4663)
                     /locus_tag="VFH83_05485"
     CDS             complement(4160..4663)
                     /locus_tag="VFH83_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6485848.1"
                     /translation="MKIGILSDTHDNLPNIRKAVELFSKNGVEAVIHAGDFCSPFTLA
                     EFKPLSSRGVKMHAVFGNNDGDHVLLIKRGTDICAFSEGACFVTLDGKRIVAVHYPEM
                     GEDLYRHGTYDLVIYGHDHKQRLEGAERRLLNPGTCSGYLAEQATVALADTRTMEIEI
                     VRIGPGA"
     gene            4740..5540
                     /gene="proC"
                     /locus_tag="VFH83_05490"
     CDS             4740..5540
                     /gene="proC"
                     /locus_tag="VFH83_05490"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET6485849.1"
                     /translation="MKRIGIVGYGVMGEAFATALARAMPQVGLLVYDARRERGEAATR
                     GWTGPAAMAVARNADEVFSGTDATLLCVKPQDMAALADEVRGSSKGRRIVSILAGRTI
                     QKIGEAFATDQVCRFMPNLAAVTGSATVGVSFHPKADAETRELGLSIAKAAGTAVEIP
                     EKLMSAITGVSGSGLAYVFAFAHAMALGGVASGFDYSTALSLAVSTLEGAASLLRKGT
                     HPQELISRVTSPGGTTIQGVRALERGGFTAAVMEAVEAAARKASDFEA"
     gene            5608..>6398
                     /locus_tag="VFH83_05495"
     CDS             5608..>6398
                     /locus_tag="VFH83_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET6485850.1"
                     /translation="MLDLKFIRDNVDAVKRNVANRKVSANPDRVVELYDRRNAILHQI
                     EALRASRNANADRMKGKLPPEERAPLIEEGKRLKDAIAEQEKQQEETERQLQAEALNI
                     PNMSHPDVPVGEGEAGNRQLRSWGSVPKFAFKPLDHVEIGQKLDMIDFESGTRVSGQK
                     FYYLKNQAVWLELALVRFALDILAKEGFTIMATPDIAREEIIAAHGFNPRGPESNIYG
                     LEGTDMCLVATAEYTLGGFHAGKVLTAEQLPLCYAGLSHCFRREAG"
BASE COUNT         1135 a         2062 c         2111 g         1090 t
ORIGIN      
        1 cgtctcgcgc gctcgcgcgc cttctcctcg ctcgaggagg ccttctccac agtcctcgac
       61 agctccgagg gcgccggtct cggcatcgtg atcctcgtgc tcatgatgaa gaagatcggg
      121 ctcagcgagg actgtttctc catcgacgca gaggacgggg agaccgtggc gagcatcacg
      181 atcccgttct ccgaggtgca catggagcag ctcgacgcgc tgacgcagct gctcgtgaag
      241 gaagtggaga cgcttcccca gttccccgac aacatcgtga acctccagag gctcatcgcc
      301 gacccgaacg cggagatcac cgatatcgcc cgggagatca gcgtcgatcc ctcgatgacc
      361 gccgacctcc tgaagctcgt caactccgcg cagttcatgc ttcccaagcg cgtggacaac
      421 atcgtggagg cggtgaagct cgtggggatc aagggcgtgc gcaacctgct gtactcgtac
      481 ggtacccaga aggtgctggg agagaagtac agcgagatgc gctccctctg gcaggactcc
      541 taccgatgcg ccttctacgc cttctccctg gcacggggct tcaagcggcg caaggagatc
      601 ctggacgatg tgtacgtcgg cgggatcctt cacgacctgg ggcagatcgt ggttgcctcc
      661 ctccatccgg atctcctgga gcgtatcacc cgcgtctgcc gggagaaggg catctcctcc
      721 gagctcctgg aggacttctc cgtcgggctc aaccacgccg aggtcggcgc gctgatcgcg
      781 cgcaagtgga acttccccga gcaactggtc ggcgccatcc gctaccacca tgagccgctg
      841 gaatgtcccg cggggctccg ggagatcgtc tacccggtct acctggccgc ggccatctgc
      901 gacattgagc gtgagcggat cgtcttcaac cagatccatc cgcatgtcct tcaggacttc
      961 aacatcgaga cccccgagca gctcgagaag atccagaccc agctcgccaa agcattcgaa
     1021 gcccagagaa ccaagttcca gtaagccgtc acccgtgcgg accgcagcgg cctgaccaac
     1081 aggcctgatc ggcggccctt gacgcccccg gatctatata gtagttttca tatgtagttt
     1141 ccccaggagg catcggatgg cgataacgat ctacacgact cccacgtgca gctactgcaa
     1201 ggtggcgaag gagttcttcc gcacccagca cgtcccgttc accgagtaca acgtggcctc
     1261 cgaccagcgc aaggcggacg agatggtccg caagagcggc cagatggggg tgcccgtgat
     1321 cgagatcaac ggcaaggtga tcgtcggttt caatcggccg gagatcgaga aggccctgag
     1381 gtgctgacgc aagccgcccg tccgttcgtc ccgtctaggt tcctggagga aagacgggat
     1441 gtacaaggct atcctgggga cggtcaccga cgtccgtcct gagcttctgg accagcgtct
     1501 acgcgtggtt gccgcggtaa ggacccaaca gggagagctt ctgcaggcgc acatgccgga
     1561 tcgggaggtg gccgcggtgc tgccgcgctc ggtactgctg ggggccgcgc ggacggctcc
     1621 gcccacgctc ctggatacgg cgcagcccat cctcgcgcgg atgaccgtgg gccgcctggt
     1681 acggctctgg cgctaccagg agcggtggtt cttctccttc cagggctgga agggcgttcg
     1741 gttcgtggag gagtcgaccc agccggctat ggccggccct gggtgacgca aacataccag
     1801 gcgtgcatca ggatcccgaa ggccacggcg acgttcaggg accgcttggc gccggccatg
     1861 ggtatggtga cacggccgcc gccggcatcc gcaagggaaa gcgcctccgg gctcaggcct
     1921 agctcctcgg agccgaccag caccagtccg cggttgggaa atgcgaaccg ctccatgggc
     1981 gtgccgccca cctcaagggc aaagatccca gcttccccgc gcaggcggtc caggtccgca
     2041 gtctcccagg ggatggcctg cccggcaccg cgggcggtcc gttccgcgcg cgggtgggtg
     2101 gggagcgggg tcctgggaga cagaaggatc cgttggacgc cgaacgcctc ggcggtacgg
     2161 aagatggacc cgacgttgta cgggctgcgg aggtcctcca ggtaaacggc caacggcagc
     2221 accgcaacct ggctgcggtc aaggcggccc gactcgggag acaccaggtc ccattcggcc
     2281 ggctcggaac ccagctcccg caggatgccg tggcgcgcct ggttgagcgc ccgcaggagg
     2341 gcggccggag gcaaggaggc gagccccgag gggagaagcg gggatcccat ccgtcggctg
     2401 gccgcgtcgt tggcgagctc ggccaggcgc gccacgtacg cggggtccgg gggcacgccg
     2461 cccatcagcc ccacctccag ggactggaga atgcgagaga tctttcgcaa acgggtacgc
     2521 ggcggaagct cgccgagctt cgtcaggggg aacaccagct cctcagtatg cggacttgat
     2581 cagctcgtag atgtcctcag aggagaccac gcgcggcagg tagttcatca tgtcgaagct
     2641 ccgggtggcg cccgccactt ccaccatctc gtcgagagag aagtcgaact cgcgaagccg
     2701 ggtaggaagg ctgagggtcg aaacgatccg tcgcaccgcc tctatggtcc gcaggggcgc
     2761 ctccgcgggg gacccttctc cgacgtccag tcgcagcagg cggcccacct ccaccagtcg
     2821 gtcgccgcgg acgttcaccg cgaactgcat ggcaaagggc agcatgatcg tggagaccca
     2881 tgacttcggc gccatgtagc gggcgttgat ggcgaaggcc agggccgagc cgatgcccag
     2941 cttgctcgtg gagagcccca gggcctggag gagcccggcc atgctcgcgc tcatccggat
     3001 gcggatatcg tcgacgttct ggagcccttc gcggatgatc ccgcccagga tctcgatggc
     3061 tcgggtcagg tacgtgtcgg acaggaaggt ggcgcgggag gacaggtacc cctcgacgca
     3121 gcccagcagc gtgtccagga tggtcgttgc ggtgtacttg gccggcagcg taagggagag
     3181 cttagggtcg atcagcgcgg cgctggtgat tccatgcggc gttcggccga tcctggggct
     3241 tcgatcccgg ccgtccacca tgaggtactc gtccacgaag aggaacggat cccggcaggt
     3301 cgtcgggatt tccacgtacg ccagggggcg ctcggaaggc tggacgccgg agaggtactc
     3361 gtcgatgtcc tggggagccg gcgcggtcat ggcgatgctc tttgcaatgg agagggtgcg
     3421 gatcccaccc aacccgatga tgacctccgc gtgggagcct cgggccaggc gcaccccgtc
     3481 gtccacggtc ctgctggtgg cgttggggac cacctcatcg aaggtgatgt agggcaggcc
     3541 cttcttgtcg aggatgtcct ggaccttgtt gatcaccttg ccttcataga ggatggcctc
     3601 cgtcaccagg agcgcccgcg cgcccagctc gctcgcgatc tgcccgatgc ggttgaccgt
     3661 gtccagcccg aagacgaccc gcgtgggaac gtgaaacagg acatctggca tggttcactc
     3721 ccttatacaa aaaaagcgga gaatcccgaa ggatacctcc gccttccacc ccagaacgcg
     3781 ccgggctcag acgccgaagg acttcatgat cccttccgcg gtcttctcga tgacctcctg
     3841 ggaaggatac gagggatcct ggagcttgcg cttgatctcg ttgatccggt ccacgcgaac
     3901 gtccggcgag cccttggccg cctccatggc gttgaagact tccgccttgg aacgggcctc
     3961 ctgagaaacg ttgatcgaat cgacgtcgct cttggccttc gtcttcgcgg gccggtcgct
     4021 cttgggtagg ttggatgctg gatcgagcgg gcctattctc tcaacggtca taggagcttc
     4081 ctttcactac ctttatcgac accccggaaa accaacttga acccttttcg accagggtac
     4141 cgccccgccc ggcggctcgt caagcgcccg ggccgatccg gacgatctcg atctccatgg
     4201 tccgggtgtc cgcaagggct accgtggctt gctccgcaag gtaaccggag caggtccctg
     4261 ggttcagcag tctgcgttcg gcgccctcca ggcgctgctt gtggtcatgg ccgtagatca
     4321 ccaggtcata cgtgccgtgt cggtacaggt cttcgcccat ctccggatag tgtaccgcca
     4381 cgatgcgctt tccatccagg gttacgaaac acgcgccctc gctgaaggcg caaatgtccg
     4441 tcccgcgttt gatcaggagc acgtgatccc cgtcgttatt gccaaaaacg gcatgcattt
     4501 tcacgccgcg gctggaaagc ggcttgaact cggcgagcgt gaagggcgag cagaagtcac
     4561 cggcgtggat gaccgcctcc acgccgtttt ttgaaaagag ctcgaccgcc ttgcggatgt
     4621 tcgggaggtt gtcgtgggta tccgagagga ttccgatctt catcgcgctt tgcctccgat
     4681 cgtcggcgtg ctgctcctgt ttctttacac gggaaaagcc ccttgctaag ctaatgccta
     4741 tgaagaggat cggcatagtg ggctatggcg taatgggaga ggcctttgcg accgcgctcg
     4801 cgagggcgat gccgcaggtg gggctgctgg tctacgatgc ccggcgcgag cgcggggagg
     4861 cggcaacccg cggctggacc ggccccgcgg ccatggccgt ggcgcgcaac gccgacgagg
     4921 tgttctccgg gacggacgct acgctcctgt gcgtgaagcc gcaggacatg gccgcgctcg
     4981 ccgacgaggt ccgaggctcc tcgaaaggcc gaaggatcgt ctcgatcctg gccggtcgca
     5041 ccatccagaa gatcggcgag gcgtttgcca ccgatcaggt ttgccggttc atgcccaacc
     5101 tcgcggccgt cacgggatcc gcgaccgtcg gcgtctcgtt ccatccgaag gcggacgcgg
     5161 agactcggga gctgggcctc tcgatcgcga aggccgccgg gacggccgtg gagatccccg
     5221 agaagctcat gtccgcgatc acgggagtct ccggctccgg gcttgcctac gtttttgcgt
     5281 ttgcccatgc catggcgctt ggcggcgtgg cttcgggctt cgactactcc accgcgctgt
     5341 cccttgcggt ctccaccctg gagggcgcgg ccagcctgct gcggaagggt acccatcccc
     5401 aggagctcat cagccgtgtc acctcgccgg ggggcaccac gatccagggt gtgcgcgcgt
     5461 tggagcgcgg cgggttcacc gccgccgtca tggaggcggt ggaggcggcg gcaaggaagg
     5521 catcggactt cgaggcgtag agtcctggcc ccgcgaaatc gaaccgccgg cgttgccacg
     5581 cccggctcgc ttcgctatga tcgccccatg ctagacctca agttcatccg ggacaacgtc
     5641 gacgcggtca aacgcaacgt cgccaaccga aaggtgagcg cgaaccctga ccgcgtggtc
     5701 gagctctacg atcggcggaa tgccatcctc caccagatcg aggcgctgcg cgcctcgcgg
     5761 aacgccaacg ccgaccggat gaagggaaag ctgccaccgg aagagcgcgc gccgctgatc
     5821 gaggaaggca agcggctgaa ggacgccatc gcggagcagg agaagcagca ggaggagacc
     5881 gagcgccagc tccaggcgga ggcgctcaac atccccaaca tgtcccaccc ggacgtgccc
     5941 gtgggggaag gggaggcggg caaccggcag ctccggagct ggggctcagt cccgaagttc
     6001 gccttcaaac ccctggacca cgtggagatc gggcagaagc tggacatgat cgacttcgag
     6061 tccggcaccc gggtgtccgg ccagaagttc tactatctga agaaccaggc cgtgtggctt
     6121 gagctggctc tcgtccgctt tgcccttgat atcctggcca aggaagggtt caccatcatg
     6181 gcgacccccg acatcgcccg ggaggagatc atcgcggccc atgggttcaa ccctcgcggg
     6241 cccgagagca acatctacgg gctcgaaggg acggacatgt gcctggtggc caccgccgag
     6301 tacacgctgg gaggcttcca cgccgggaag gtgctgaccg ccgagcagct gccgctgtgc
     6361 tacgccggcc tctcccactg cttccgccga gaggccgg
//
LOCUS       DASRZS010000217         3969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_510142, whole genome shotgun sequence.
ACCESSION   DASRZS010000217 DASRZS010000000
VERSION     DASRZS010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510142"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFH83_05500"
     CDS             <1..290
                     /locus_tag="VFH83_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485851.1"
                     /translation="LMQWKADFWAAVDARDSALAIEQALVRDYGGSHALFLTDAHNWT
                     GVPSRALARMFYPEVTTWKRPVEGTETLVSIERARSLLGYEPRYSASRFFE"
     gene            283..1488
                     /locus_tag="VFH83_05505"
     CDS             283..1488
                     /locus_tag="VFH83_05505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6485852.1"
                     /translation="MSKPQRFLTRPIVTLLAIQLVTGVLVSPQYTFFPIYLQELGFSA
                     VALSLIVALQRLTALFTSIGGGALADGMGAKRVLIVSQLAYFAGTVVFSLSGAWQISL
                     FWAVSGIGLGLNSMSSQSYLLEKASPARLGLMTALLYLGYTIGAAIGNPLVGALLQRA
                     GYARLAPVAVVPAAATLVFTGLALPRSAVAAASVGGWKARLVADVKGYRTVAARPGLT
                     LLGILRFLPTFSYGMLLVFVPLQLKGAGATKELVALYATVNSICASLAQLLVGRIADK
                     AGWRAPLAVVFATFTVSAILVGVLPGNLWVVFVAAVIAMSAAWSLSTLGPMLVSDAVA
                     QGERGRALGFIQLFWCVAMIAGGLIGGSLYELWIGLPMTVGGVVTGSALLLLPTLSRR
                     LRARRAEAR"
     gene            complement(1494..2585)
                     /locus_tag="VFH83_05510"
     CDS             complement(1494..2585)
                     /locus_tag="VFH83_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485853.1"
                     /translation="MRWEYSEWDQVLQDLLKSFQDLMSLFNYLLLMAAGDVDKVFQWM
                     RYLQERGSLGEEVDLEEFRRQLEQQRIIERLKEGGYSLTAKGEQVIRKDSLNQIFQSL
                     QKSGFGQHRVPATGNGDERLTETRPWRFGDPVTQIDALGTISNAVKRAGLDEIQLAEE
                     DLEVYEAEHLSACATMLLIDISHSMILYGEDRITPAKQVALALTELILTRYPKDVLRV
                     GTFGDDAKEIAISDIPYLQVGPFHTNTRAGLEMGQSILEASRQSNRQIFMVTDGKPTA
                     ITENGEVYKNPYGFDPKILAQTLEAAAACRKAGITITTFMLARDPELVSFVDELTRLN
                     RGRAYYASADKLGEFLFADYIRNKRRVLH"
     gene            complement(2593..>3969)
                     /locus_tag="VFH83_05515"
     CDS             complement(2593..>3969)
                     /locus_tag="VFH83_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HET6485854.1"
                     /translation="IGYDTTVIPGLVNAILSQHDFILLGLRGQAKTRILRGLVRFLDE
                     QLPVIADSEVNDDPFAPVSRRGREMLASKGKDTPIEWVPREARYREKLATPDVTIADL
                     IGDIDPIKAATRKLAYADEDVIHFGIIPRTNRGIFAINELPDLPPRIQVGLLNIMEEK
                     DIQIRGFPIRMPLDILMVYSANPEDYTNRGNIITPLKDRIDSQIITHYPNDRDAAMRI
                     TAQEAWTARESGIDLTMPDWFRELVEEVAFAARKSEYVNQASGVSARMSISLLENVYS
                     NMERRGILRGEKRVYPRIADLHSAVTAITGKVELVYEGEQQGATIVARKIIGEAVKST
                     FRRKFPDPAPKKKKKDGGEEQGRAENSPFQPILNWFSKGNTVETSDDMAYDEYVKRLQ
                     MVNGLKEVTAKHLELKETDGLPLGMEFVLEALHQHSMVSKREEGVKTAYRDMLKAMFD
                     HMATDEES"
BASE COUNT          565 a         1295 c         1311 g          798 t
ORIGIN      
        1 agctcatgca gtggaaggca gacttctggg cagcggtgga cgcccgggac agcgccctgg
       61 ccatcgagca ggccctggtg cgggactacg ggggcagtca cgcgctgttc ctgacggacg
      121 cgcacaattg gacgggagtg ccgtctcggg cgcttgcccg gatgttctac ccggaggtga
      181 ccacctggaa gcggccggtc gagggaaccg agacgctggt gagcatcgag cgcgcgcggt
      241 cgcttctggg ctacgagccg cggtattccg cgtcacgctt ctttgagtaa gccccagcgc
      301 ttcctcaccc ggccgatcgt cacgctcctg gccatccagc tcgtgaccgg ggtcctggtc
      361 tctccccagt acacgttctt cccgatctac ctccaggagc tgggcttctc cgcggtcgcg
      421 ctttcgctga tcgtggcgct tcagcgattg accgcgctct tcacctccat cggcggcgga
      481 gcgctggcgg acgggatggg cgccaagcgg gtcctgatcg tcagccagct cgcctatttc
      541 gccggcaccg tcgtgttctc cctaagcggc gcctggcaga tctcgctgtt ctgggccgtg
      601 agcgggatcg ggctgggcct gaactccatg tccagccaga gctacctgct ggagaaggcc
      661 agcccggcgc gactggggct catgaccgcg ctcctctacc tggggtacac catcggtgcc
      721 gccatcggaa acccgcttgt cggcgcgctg ctgcagaggg ccgggtacgc caggctggcg
      781 ccggtagccg tggtgccggc cgccgccacg ctcgtgttca cgggtcttgc gctgccccgg
      841 tccgccgttg cggccgcgtc cgtcggtgga tggaaagctc ggctggtagc cgacgtgaag
      901 ggataccgaa cggtggcggc tcgcccaggg cttaccctcc tggggatcct ccggttcctc
      961 ccgacgttca gctacgggat gctcctggtg ttcgtccccc tacagctcaa gggggccgga
     1021 gccaccaagg agctcgttgc cctctacgcc acggtgaaca gtatctgcgc gtccttggcc
     1081 cagctcctgg tgggtcgcat cgcagacaag gcgggatgga gggcgcccct ggcggtcgtc
     1141 ttcgccacct tcaccgtctc ggcaatcctc gtcggggttc tccccggcaa tctctgggtg
     1201 gtcttcgtcg ccgcggtgat cgcgatgagc gcggcctggt ctctctccac cctcggcccg
     1261 atgctggtgt ccgatgccgt ggctcaggga gagcggggcc gggctctggg attcatccag
     1321 ctcttctggt gcgtggccat gatcgcgggc ggcctgatcg gcgggtctct ctacgagcta
     1381 tggatcggtc ttcccatgac cgtgggaggc gtggtgacgg ggtcggcgct cctgctgcta
     1441 cccacgctgt cgcgacggct cagggcgagg cgcgccgaag cccgctagta ccgctagtgc
     1501 agcacgcgcc tcttgttgcg gatgtagtcc gcgaagagga actccccgag cttgtccgcc
     1561 gaggcgtagt aggcgcgtcc ccggttcagc cgggtcagct cgtctacgaa gctcaccagc
     1621 tcgggatccc gggccagcat gaacgtggtg atcgtgatcc ccgcctttcg gcaggctgcc
     1681 gcggcctcca gcgtctgggc caggatcttg ggatcgaagc cgtaggggtt cttgtagact
     1741 tccccgttct ccgtgatggc ggtgggcttg ccgtcggtca ccatgaagat ctggcggttg
     1801 gactggcggg aggcctccag gatggactgc cccatctcca gtccggcccg cgtgttcgtg
     1861 tggaaggggc cgacctgaag gtacgggata tcgctgatcg cgatctcctt ggcgtcatcc
     1921 ccgaaggtgc ctacccgaag cacgtccttg gggtaccggg tgaggatgag ctccgtcagc
     1981 gccagggcca cctgctttgc gggcgtgatc cgatcctcac cgtacaggat catgctgtgg
     2041 ctgatgtcga tcaggagcat ggtggcgcac gcggagaggt gctccgcctc gtacacctcc
     2101 aggtcctctt ccgcgagctg gatctcgtcc agcccggccc gcttcaccgc gttggagatg
     2161 gtccccagcg cgtcgatctg ggtcaccggg tccccgaacc gccagggccg ggtctccgtg
     2221 agccgctcat cgccgttgcc cgtggccggg acccggtgct ggccgaaccc cgacttctgc
     2281 aggctctgga agatctggtt gagcgaatcc ttgcggatga cctgctcgcc ctttgccgtc
     2341 agcgagtagc cgccttcctt cagccgctcg atgatgcgct gctgctcgag ctgccggcgg
     2401 aactcctcca ggtccacttc ctcacccagg gagccgcgct cctggagata gcgcatccac
     2461 tgaaagacct tgtccacgtc cccggccgcc atgagcagca ggtagttgaa caggctcatc
     2521 aggtcctgga agctcttcag cagatcctgg aggacctggt cccattcgga gtattcccaa
     2581 cgcattaggg gcctacgact cctcgtcggt tgccatgtgg tcgaacatag ccttcagcat
     2641 gtcgcggtag gcggtcttca cgccctcctc ccgcttggat accatggagt gctggtggag
     2701 cgcttccagc acgaactcca tccccagggg aaggccatcg gtttccttca gctccaggtg
     2761 cttggccgtg acctccttca gcccgttcac catctgcagg cgcttgacgt actcgtcata
     2821 cgccatgtcg tccgaggtct ccacggtgtt gcccttggag aaccagttca ggatgggctg
     2881 gaagggggag ttctccgcgc gcccctgctc ctctccgccg tccttcttct tctttttcgg
     2941 cgccggatcg gggaacttgc ggcggaaggt gctcttgacc gcctccccga tgatcttgcg
     3001 ggccacgatg gtggcgccct gctgctcccc ttcgtagacc agctccacct tgccggtgat
     3061 ggcggtgacg gcggagtgca ggtccgcgat ccgcgggtac acccgcttct cgcccctcag
     3121 gataccccgc cgctccatgt tggagtagac gttctccagg agagagatgc tcatgcgcgc
     3181 cgagacgccg gaggcctggt tcacgtactc gctcttgcgg gccgcgaagg cgacctcctc
     3241 caccagctcg cggaaccagt ccggcatggt gaggtcgatc ccgctctccc gggcggtcca
     3301 ggcctcctgc gccgtgatgc gcatggcggc gtcccggtcg ttgggatagt gggtgatgat
     3361 ctggctgtcg atccggtcct tgaggggcgt gatgatgttc ccccggttgg tgtagtcctc
     3421 cgggttggcc gagtacacca tgaggatatc cagcggcatg cggatgggaa atccgcggat
     3481 ctggatgtct ttctcttcca tgatattgag caatccgacc tggatgcggg ggggaaggtc
     3541 cggcagctcg ttgatggcga agatcccccg gttggtccgg ggaatgatcc cgaagtggat
     3601 gacgtcctca tccgcgtagg ccagcttgcg ggtggcggcc ttgatgggat cgatgtcccc
     3661 gatgaggtcg gcgatggtca catccggggt ggccagcttc tcccggtagc gggcctcccg
     3721 cggcacccac tcaatggggg tgtccttgcc tttggaggcc agcatctccc ggccccgccg
     3781 gctcaccggc gcgaaaggat catcgttgac ctcggagtcc gcgatgaccg gaagctgctc
     3841 gtccaggaag cgaaccaacc ctcgaaggat gcgggtcttg gcctggcctc gcagtcccag
     3901 gaggatgaag tcgtgctggg agagaatggc gttcaccagg cccgggatca cggtggtgtc
     3961 gtagccgat
//
LOCUS       DASRZS010000218         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_124862, whole genome shotgun sequence.
ACCESSION   DASRZS010000218 DASRZS010000000
VERSION     DASRZS010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124862"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VFH83_05520"
     CDS             complement(<1..757)
                     /locus_tag="VFH83_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6485855.1"
                     /translation="MQYQKVGRSNLTASRICLGTMHFGGVADEATCHRIMDRALDLGI
                     TCFDTANVYGMPAARGRSEEIIGRWFAQKGGRRERVVLATKLYAKMVEGDVPNEEPGI
                     SAHKIRRHAADSLRRLQTDHIDLYQVHHIDRHIPLEEFWGAFELLRQRGDILYVGTSN
                     FPGWGLAKFQMAASFRGILGIVSEQSQYNLLNRWPELEVIPAARDFGIGIMAYMPLAG
                     GLLTGKRSAPAGSRTARVEGEYKRSLAGDAVFTE"
     gene            complement(762..1640)
                     /locus_tag="VFH83_05525"
     CDS             complement(762..1640)
                     /locus_tag="VFH83_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485856.1"
                     /translation="MPETDARAPGKLVRPFSARRVIGAQVLPRIVLILICLVYFAPFY
                     WMVITSFKDNLQLSAFPPIFWPHPFVWRNYLDAVNYIPFFRFFINSLILTAGATVGAV
                     LSNSIIAYGFSRIEWKGREVLFYVAIATVFIPFPVTLVALFDIFAKIHWINTFRPLIV
                     PTFFGQAFYMFLIRQFLMGIPKELSDASRIDGANEFTIFRLIILPITKPAIAVVAIFA
                     AVNAWNDFLTPLIYLSEQSRYPLSIGLQFFKAEHTVAFSLLMAASTLVVLPVIVLFLF
                     FQRFFIEGVTVGAIKG"
     gene            complement(1657..2547)
                     /locus_tag="VFH83_05530"
     CDS             complement(1657..2547)
                     /locus_tag="VFH83_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485857.1"
                     /translation="MSTDRRNLRNGLLFISPWLIGFAAFQVYPILYSLRLSFTEYSGF
                     GAWVDTGVQNYTRLLRDPLFWKAAYNTLYYTVLAVPIGVVVAIVLALAMNQRVREISA
                     YRAILYLPSILPLFALSFVWVVFVNPRYGMLSYVLNVVGIPAPDWIGDPAWTKLSIVI
                     LAQLGAGGPALIFLAGIRAIPRDLFESASLDGAGPFRRFFSITLPLITPVILYDVILG
                     LSYGMQVFTQAYIITSAGQYTAGPLNSLLFYVFYIYKTAFQYTQMGYAAALVWVLFIV
                     SMILALAVFRWARSWVHYEV"
BASE COUNT          542 a          793 c          903 g          428 t
ORIGIN      
        1 actcggtgaa caccgcgtcc ccggcgaggg atcgcttgta ttccccctcg actctcgcgg
       61 tccgagaccc cgccggagcg ctccgcttgc cggtgagaag ccctcccgcc agaggcatgt
      121 acgccatgat tccgatcccg aagtcccggg ccgccggaat gacctccagc tccggccagc
      181 ggttcaggag gttgtactgg gactgctcgg agacgatccc caggattccc cggaaggagg
      241 ccgccatctg gaacttggcc aacccccagc cggggaagtt gctggtgccg acgtaaagga
      301 tgtctccccg ctggcggagc agctcgaagg cgccccagaa ctcttccagc gggatgtggc
      361 ggtcgatgtg gtggacctgg tagaggtcga tgtggtccgt ctggaggcgc cggagggagt
      421 ccgccgcgtg tcggcggatc ttgtgcgccg agatccccgg ctcttcgttg gggacatcgc
      481 cctccaccat cttggcgtac agcttggtgg ccaggaccac gcgctcccga cggccgcctt
      541 tctgggcgaa ccagcggccg atgatctcct cactgcgtcc gcgggcggcg ggcatgccgt
      601 agacgttggc ggtgtcgaag caggtgatgc ccaggtccag ggcccggtcc atgatccggt
      661 ggcaggtggc ctcgtccgcc actccgccga agtgcatggt tcccaggcag atccggctcg
      721 ccgtcaggtt ggatcgaccg accttctggt actgcatgac gctaaccctt gatggctccg
      781 accgtcacgc cctcgatgaa gaacctctgg aagaacagga acagcacgat gaccggcagc
      841 accacgagcg tcgatgccgc catgaggagg ctgaacgcca ccgtgtgctc cgccttgaag
      901 aactgcaggc caatggagag cgggtagcga ctctgctccg agaggtagat cagcggggtc
      961 aggaagtcgt tccaggcgtt caccgccgcg aagatggcca ccaccgcgat ggcgggcttc
     1021 gtgatgggca ggatgatgag gcggaagatc gtgaactcgt tcgcgccgtc aatgcgcgac
     1081 gcgtcggaga gctccttggg aatccccatg agaaactgcc gaatgaggaa catgtagaac
     1141 gcctggccga agaacgtggg cacaatcagg ggtcggaacg tgttgatcca gtggatcttc
     1201 gcgaaaatgt cgaacaacgc cacgagcgtc acggggaacg ggatgaagac ggtcgcaatg
     1261 gcgacgtaga acaggacctc ccggcccttc cattcgatgc gggagaatcc gtaagcgata
     1321 atggagttgg aaaggaccgc cccgacggtg gctcccgcgg taaggatcaa ggaattgatg
     1381 aagaaccgga aaaagggaat gtagttcacc gcgtccaggt agtttcgcca cacgaacggg
     1441 tgaggccaga agatgggagg gaaggcgctg agctggaggt tgtccttgaa cgacgtgatg
     1501 accatccagt agaagggcgc gaaatagacc agacagatca ggatgagcac gatcctggga
     1561 agaacctgcg ctccgatcac ccgtcgggcg gagaaaggac ggaccagttt cccgggggcc
     1621 cgggcgtcgg tttccggcat cctcttcgct cctttcctac acttcgtagt ggacccagct
     1681 tcgggcccac cggaagaccg cgagcgccag gatcatgctg acgatgaaaa ggacccacac
     1741 gagagccgca gcgtagccca tctgcgtgta ctggaaggcg gtcttgtaga tgtaaaagac
     1801 gtaaaacagg agcgagttca ggggtcccgc cgtgtactgc cccgcgctgg tgatgatgta
     1861 cgcctgggtg aagacctgca tgccgtagct caatcccagg atgacgtcgt acaggatcac
     1921 cggcgtgatc agcggcaggg tgatgctgaa gaaccggcgg aacgggccgg ccccgtccaa
     1981 ggacgcggac tcaaagaggt cccgggggat cgcacggatc ccggcgagaa agatcagggc
     2041 gggtccgccg gcgccgagct gcgccaggat gacaatggag agcttggtcc aggcgggatc
     2101 gccaatccag tccggggcgg gaatgcccac gacgttcagc acgtagctca gcatcccgta
     2161 gcgggggttc acgaacacca cccagacaaa ggagagggca aacaggggaa ggatggaggg
     2221 gaggtagagg attgcccggt acgcggaaat ttccctgacg cgctggttca tcgccagggc
     2281 cagcacgatg gccaccacca ccccgatggg caccgcgagc accgtgtagt acagcgtgtt
     2341 gtacgccgcc ttccagaaca gcgggtcacg cagcagccgc gtgtagttct gaaccccggt
     2401 gtctacccac gcgccgaacc ccgagtactc ggtgaagctg agccgcagcg agtacaggat
     2461 gggatagacc tggaatgcgg cgaagccgat gagccacggg ctgatgaaca ggagcccgtt
     2521 cctgaggttc cgtcgatcgg tgctcatgcc gcctccctgg ttcgcggtgg gtatgtgtgg
     2581 agccatggtg ccggtgcgcg gctctccgcc gccgcgcgcc ggcccgtgac gtcacccccg
     2641 gcgcgcgagc cgggggtgac acggtg
//
LOCUS       DASRZS010000219         8254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2431531, whole genome shotgun sequence.
ACCESSION   DASRZS010000219 DASRZS010000000
VERSION     DASRZS010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2431531"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /gene="fusA"
                     /locus_tag="VFH83_05535"
     CDS             <1..336
                     /gene="fusA"
                     /locus_tag="VFH83_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526182.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET6485858.1"
                     /translation="STVIAFEAAGALAFDGACRKASPILLEPIMAVDVLTPTEFLGEV
                     ISNLNARSGLILNVENKPGADHVHAQSPLVSMFGYSTALRSATQGRATFTMEFSHFAE
                     KVGGFGDSR"
     gene            455..2518
                     /locus_tag="VFH83_05540"
     CDS             455..2518
                     /locus_tag="VFH83_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET6485859.1"
                     /translation="MSVSTRSLRNVAFAGHVGTGKTTLVEAILAAGGVIPKAERIETG
                     RTVSDFTDEEISRKISIHTSLSHVSAGDVKINILDTPGSADFVGEVAAAFRVAECAGL
                     VVGADVGVQIETLKVWRRLLRLELPRFAFVNKMEKERADFSASVADLSDKLKATFVPV
                     TLPIGAGAGFTGVVDLVDQKAYTDGKAGDVPAGMKAEVEAARARLIESAAEGDDALIE
                     KFFAEGSLTPEEIRKGLAGGMKSGKLVPVLCGAGLTGAGVACLLDFISYAVSAPGGTV
                     KGKAGDAETEREVAESGPASCFVFKTAMDQFTGKLSFFKVLSGKIGTDTELVNAREGK
                     KEKITKLYTTQGKRLEETPDLSAGDIGLITKSASLRTNDTLHAPDAAILYPPLELPTP
                     VHALAISAKAKKDEDKMNQMLQRTMEEDLTFQVSYEKETKETVISGMGELHISMILDK
                     IRENQKIDMETRIPRVAYRETIQGKADAEYTHKKQTGGHGQYAKVSLEVNPIPRGERY
                     KFTNAIFGGAISRGYIPGVEKGVVEAMEAGVLAGYPVVDVEARVVDGKEHPVDSSELA
                     FKLAAREAFRECMRNAKPVLLEPVCNLTVFVDEQYLGAVLSDLSSKRGKVSGQTPIGG
                     GILEIKAQVPQAELMRYAIDLKALTAGTGSFELEFDHYSPISGRIAEDVIKAAQAAKT
                     EEKKE"
     gene            complement(2596..3003)
                     /locus_tag="VFH83_05545"
     CDS             complement(2596..3003)
                     /locus_tag="VFH83_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485860.1"
                     /translation="MLQFYFLSITANVLSGLTLAGDAIGRRMPGFAELNARLSGRRGK
                     LIVGLASLLVGFCTLFVPAEPPLILGDLLPSAVGMVMGIALLFEVFRDMATLPAEQSE
                     RSERVPVGYRTTLGIIGLVTAGVHFFLPDRIIL"
     gene            complement(3061..3621)
                     /locus_tag="VFH83_05550"
     CDS             complement(3061..3621)
                     /locus_tag="VFH83_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485861.1"
                     /translation="MKPPMLRVRRDAGRDSPGLYRVGSEGEPAAGERDRRREDERARE
                     PRRSGRRGAVVGSLLAGRLTFIAVLVLVIGAFALLRFAGNRKPDRATLSGWQVSLSAT
                     VVDGSLSASLSFRPRGRQARPAAAASVRFFLPETGGEARVGETLVQDPVVARVVLPLT
                     GVEKRLQADVRIAGATATLSASIRQP"
     gene            complement(3637..5190)
                     /locus_tag="VFH83_05555"
     CDS             complement(3637..5190)
                     /locus_tag="VFH83_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET6485862.1"
                     /translation="MKVVSSAAMAAIDQRTQTEYSIPGALLMEDAGTKAWLLLRQDYD
                     GGVPSGRVVVLAGKGNNGGDGFVMARQAAVDGVRRLTVVLAGGRPPESGDAGRMLAAC
                     QRLPLEILAWPDQADAVRSALGEAELVLDCIAGTGIRGPLKAPLSDLVSVVNSGSAAR
                     IVAIDVPSGVGDEFRTGMPAVRAGKTLTMGLPKLCLYLPNARTLCGEIQVVPVGFPPA
                     LVDDPEIPGEMLQLSGWRRLARPIPADTYKNRRGHLSVFAGSVGTTGAAWLAASAAAR
                     CRLGLVTLFVDRDVYPVAGSRMASVMCRPWDSAPEGPPGWDPAAFSAVLVGPGWGLTE
                     AKAQWLRRLLTLPIGGVLDADGITLLSRFREERGVGLHGRWVLTPHPGEFSRLLSVPR
                     DRVLDDPVGQALAAASRMEAVIVLKGHCTVVAAPDGKYWILDAPNPALATGGAGDVLA
                     GIVAAGVAGGLTPVDAARFGVALHANVARLAWRRNGWFLSEDLVPMLSRLLGRADAGR
                     RRGDAPGSR"
     gene            complement(5194..6414)
                     /locus_tag="VFH83_05560"
     CDS             complement(5194..6414)
                     /locus_tag="VFH83_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485863.1"
                     /translation="MVSHFRSLRRFLLVIPLLLVLAACGRKGLEILVSEPLFSLNLGR
                     LDNQIDLFRPGEAAREGENHMVMRDGWFYVANGPARKVMVFSSYGDLLFLLYNPQTNP
                     TPTLLGSPTTGAEEASTRGAVAYPFNDIGQIAVSSDKTLYVEDSVADAKAVQDTERGV
                     LQSRVILRFDRKGKPLGYIGQEGPGGTPFAFIYALYVTNRDDLVVVSRLPASWETFWY
                     SSDGKLLYRREVDQAHLPVQASGTLVPTIVNILPDLQSPVLYLCINLYRADPDSVSSS
                     GQDTVLARAYRLNVATGRYESYVEFPQNPPTRQKEGLQTTVIPSPPSDLMGVSSDGSL
                     FLLAFTDANLYTLQILDRSGRIVASRRMVIEDSELAYREVHLSSTGILYGLLADQTKA
                     RITWWRSDQLAGRE"
     gene            6478..>8254
                     /gene="secA"
                     /locus_tag="VFH83_05565"
     CDS             6478..>8254
                     /gene="secA"
                     /locus_tag="VFH83_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET6485864.1"
                     /translation="MLQGVLTFFLGSKHEHDLKSLAPFVQRIDALELEVMGYPDEKFP
                     AKTVEFRQRLAAGERLDDLLPEAYALVREAARRRLGERIFDVQMMGAVVLHQGKITEM
                     KTGEGKTLASVPAAYLNSLTGMGVHVITVNDYLAERDAAWMGPIYGLLGVSVGVILSQ
                     MDNDQRKISYSKDITYGTNNEFGFDYLRDNMCWSPSDKVQRGHAYAIVDEIDNILIDE
                     ARTPLIISGMAEDDTFKFAEVNRLVEQLEECEKDPATGTYPDGDAQPKGDFKLEEKNK
                     RVTFTDQGMNHIESLLMKRGLIRDSLFVSENFEYIHYFTQALRAHRLYAKDVDYVVQD
                     GKVQIVDEFTGRILYGRRYSEGLHQAIEAKERIKIAARNRTLATITFQNYFRMYGKLA
                     GMTGTADTEAQEFAKIYNLDVVVIPTNRPVVRDDADDEIYLTEEEKLTAICNAIGELH
                     AKGQPVLVGTISIEKSERLSRMLTGRGIRHEVLNAKNHAREALIIAEAGTRGSVTIAT
                     NMAGRGTDIKLGGNPEFRARKKAGTQATEDVYRHALTEEYGRWQQDYQGVRGLGGLHV
                     LGTERHEARRIDNQLRGRSGRQGDPG"
BASE COUNT         1569 a         2728 c         2756 g         1201 t
ORIGIN      
        1 tccacggtga tcgcgttcga ggcggcggga gcgctggcgt tcgacggggc ctgccgcaag
       61 gcgagcccca tcctccttga accgatcatg gccgtggatg tcctgacgcc aacggagttc
      121 ctgggagagg tcatctccaa cctgaacgcc cgctcggggc tcatcctgaa cgtggagaac
      181 aaacccggcg cggaccacgt gcacgcccaa tcgccgctgg tgtccatgtt cggctattcg
      241 acggccctgc gcagcgccac ccagggaagg gcgacgttca ccatggagtt ctcccatttc
      301 gccgagaagg tcggcggctt cggcgactct cgttgaggat cgccggcgaa ccgcgggtag
      361 gcggcgcctg acgccatccg tagttccctt tgcttgacaa aagggggcta gcgaactaca
      421 gtacgcgaac cctcgacacg taaggagtca cacgatgagt gtttccacac gctccctgcg
      481 aaacgtagcc ttcgccggtc acgtcggaac gggcaagact acgctggtcg aggcgatcct
      541 cgccgccggg ggagtcatcc ccaaagccga gcggatcgag accggtcgaa cggtcagcga
      601 tttcaccgat gaggagatct cccgcaagat ctccatccac acctccctga gccacgtctc
      661 cgcgggggac gtgaagatca acatcctgga caccccgggc tcggccgact tcgtcggtga
      721 ggtggccgcc gcgttccgcg tcgccgagtg cgccggtctg gtggtcggcg cggatgtggg
      781 cgtccagatc gagaccctca aggtgtggcg ccgcctgctc cgcctcgagc tgccgcgctt
      841 cgcgttcgtc aacaagatgg agaaggagcg cgcggacttc tcggcgagcg tggcggacct
      901 ctcggacaag ctcaaggcca ccttcgttcc cgtcacgctg cccatcggtg ccggagccgg
      961 attcacgggg gtcgtggacc tggtggacca gaaggcctat acggacggaa aggccgggga
     1021 cgtgcccgcg ggcatgaagg ccgaggtgga agcggcccgg gccaggctca tcgagagcgc
     1081 ggcggaaggg gacgacgccc tcatcgagaa gttcttcgcc gaagggagcc tgacgccgga
     1141 agagatccgc aaaggcctgg ccggcggcat gaagagcggg aagctggtgc ccgtcctctg
     1201 cggcgccggg ctcaccggag ccggtgttgc ctgcctgctt gacttcatct cctacgcggt
     1261 ttccgccccc ggcggcacgg tgaaggggaa agccggcgac gcggagacgg agcgtgaggt
     1321 ggccgagtcc ggtcccgcct cctgcttcgt gttcaagacc gccatggacc agttcacggg
     1381 caagctctcc ttcttcaagg tcctctcggg gaagatcggg acggacaccg agctggtgaa
     1441 cgcgcgcgaa ggaaagaagg agaagatcac caagctgtac acgacccagg gcaagaggct
     1501 ggaggagacg ccggacctga gcgccggaga catcgggctc atcaccaaga gcgcttccct
     1561 gcggaccaac gataccctcc acgcaccgga tgccgcgatc ctgtaccctc ccctggagct
     1621 tcccaccccc gtccatgccc ttgccatctc ggcaaaggcc aagaaggacg aggacaagat
     1681 gaaccagatg ctccagagga cgatggaaga agacctgacc ttccaggtct cctacgagaa
     1741 ggagaccaag gagacggtca tttcgggaat gggagagctc cacatctcga tgatcctgga
     1801 caagatccgc gagaaccaga agatcgacat ggagacccgg atcccgcggg tggcctaccg
     1861 ggagaccatc cagggcaagg cggacgcgga gtacacccac aagaagcaga ccggcggcca
     1921 cggccagtac gccaaggtgt ccctggaggt caaccccatt cctcggggag agcgctacaa
     1981 gttcaccaac gccatctttg gcggcgcaat ctccaggggg tacatcccag gcgtcgagaa
     2041 gggcgtagtc gaggccatgg aagccggcgt cctggccggc taccccgtgg tggatgtaga
     2101 ggcccgggtg gtggacggca aggaacaccc cgtagactcc tcggagctgg cattcaagct
     2161 cgctgctcga gaggccttcc gcgagtgcat gcggaacgcc aaaccggtgc tgctggaacc
     2221 ggtgtgcaac ctgaccgtgt tcgtcgacga gcagtacctg ggcgcggtgc tatcggacct
     2281 cagctccaag cgggggaagg tgtccgggca aacgcccatc ggcggaggga tcctggagat
     2341 caaggcacag gtgccccaag ccgagctgat gcgatacgcc atcgacctga aggcgcttac
     2401 cgcgggcacc ggttcattcg agttggagtt cgaccactac agcccgatct ccgggaggat
     2461 cgccgaggac gtgatcaagg ctgcccaggc cgcaaagacg gaggagaaga aggagtaggc
     2521 agggcgcggc ttcgccgtcc gtggctacag ccgcgctttc gccttccctg gctcgcgcgg
     2581 cttcgccgtc cgtggctaca aaatgatgcg gtccggcagg aagaagtgca ctcccgcggt
     2641 caccaaccct atgatcccca gtgtagttcg gtagcccacc gggacccgtt cactccgctc
     2701 ggattgctcg gcggggaggg tggccatgtc tcggaacacc tcgaacagga gcgcaatccc
     2761 catcaccatc cctaccgcgg acggaagcag gtctcccagg atcaggggcg gctcggcggg
     2821 aacgaagagg gtgcagaacc ccaccagaag ggaagccagc ccgacgatca gcttgccgcg
     2881 tcggccggac agccgtgcat tgagctcggc gaaccctggc atccgacggc cgatggcgtc
     2941 cccggccagc gtgagtcccg agagtacgtt tgcggtgatc gagagaaagt agaactggag
     3001 catccgtttt ggcgcggctc tatgccgctc cctcgatgat acggcccggc gccatcgagg
     3061 tcaaggttgc cggatcgacg cgctcagcgt cgccgtggcg ccggcaatcc gtacgtcggc
     3121 ctggagccgt ttctcgacac ccgtgagggg caacaccacc cgggccacga ccggatcctg
     3181 caccagggtc tcccctaccc tcgcttctcc tccggtctcc gggaggaaga atcggaccga
     3241 cgccgcggcc gcagggcgcg cctgacggcc tcgaggacgg aagctgagcg acgcggagag
     3301 agatccgtcg accacggtgg cggagaggga gacctgccaa ccggagaggg tggcacggtc
     3361 cggtttgcgg ttccccgcaa atcgcaggag cgcaaaggcc ccgatcacca ggaccagaac
     3421 cgcgatgaac gtcagccgcc cggcaaggag ggacccgacg acggctccac gacgcccgga
     3481 gcgccgcggc tctcgtgctc gttcgtcttc ccgtctccgg tcccgttctc cagccgcggg
     3541 ctcaccttcc gagccgacgc ggtagagtcc cggggaatcg cgcccggcat ccctgcgaac
     3601 ccgaagcatc gggggcttca cgttccggcc gctccttcag cggcttcccg gggcgtcgcc
     3661 gcgccgccgg ccagcgtccg cgcgacccag gagacgcgag agcatgggaa cgaggtcttc
     3721 cgagagaaac cagccgtttc gtcgccaggc caggcgcgcg acattcgcgt gcaatgccac
     3781 gccgaaacgc gccgcatcca cgggcgtcaa cccccccgcc acaccggcgg ccacgatgcc
     3841 ggcaagcacg tcgccggccc cgccggtggc cagagctgga ttcggcgcgt ccaggatcca
     3901 gtacttccca tcgggcgcgg ccacgaccgt gcagtgtcct ttgagaacga tcacggcctc
     3961 catccgggaa gccgccgcga gtgcctgacc cacgggatca tccagcaccc ggtcccgagg
     4021 caccgacagg agacgggaga actccccggg atgcggcgta aggacccatc gaccgtggag
     4081 ccccacgcca cgctcttccc gaaaccgtga gaggagcgtg atgccgtcgg cgtccaggac
     4141 tccaccgatg ggaagcgtca agagacgccg aagccattgg gctttggctt ccgtcaaccc
     4201 ccacccgggc cctaccagga cggcggagaa ggcggcaggg tcccagcccg gcggaccctc
     4261 tggagcactg tcccacgggc ggcacatgac ggaggccatc cgagatcccg cgacgggata
     4321 gacgtcccgg tccacgaaga gagtgaccag gccgaggcgg caccgggccg cggcgctggc
     4381 ggcaagccac gcggcgcccg tggtgccgac cgacccggcg aacacggaca ggtgacccct
     4441 tcggttcttg taggtatccg cggggatggg gcgagcaagc cggcgccagc cggagagttg
     4501 cagcatctcg ccggggatct cggggtcatc caccagcgcc ggcgggaatc ccacgggaac
     4561 cacctggatc tctccgcaga gggtccgcgc gttgggcaga tacaggcaca gcttgggcag
     4621 ccccatggtg agggtcttgc ccgcgcgcac cgccggcatg ccggtgcgga actcgtcccc
     4681 caccccactg ggcacatcga tggccacgat ccgtgccgcg gagccggagt tcaccacgga
     4741 gacgaggtcg gaaaggggcg ccttcagcgg accgcggatg cccgtcccgg cgatgcagtc
     4801 caggaccagc tcggcctccc ccagcgcgga gcgcacggca tccgcctggt ccggccaggc
     4861 caggatctcc agagggagtc tctggcaggc ggccagcatc ctcccggcat ccccgctctc
     4921 gggcggtcga ccgccggcca gcacgaccgt gagccgtcgg acgccatcga cggctgcctg
     4981 ccgcgccatg acgaacccgt ctccaccatt gtttcccttg ccggccagca ccaccacccg
     5041 gccggacggc acgccgccgt cgtagtcctg acgaagcagg agccaggcct tggtcccggc
     5101 gtcttccatg agaagcgcgc ccggaatcga gtactcggtc tgggtgcgtt ggtcgattgc
     5161 cgccatggcg gcggagctca cgaccttcac cgactactcc cggccggcca actggtccga
     5221 ccgccaccag gtgatccggg ccttggtctg gtccgccagg agcccgtaca ggatgccggt
     5281 ggaggagagg tgcacctccc ggtacgccag ctccgagtcc tcgatcacca tcctccggct
     5341 ggcgacgatc cgcccggacc ggtccaggat ctggagggtg tacaggttgg catccgtgaa
     5401 ggcgagaagg aacagcgagc cgtccgagct cacgcccatg agatcgctgg gcggggaagg
     5461 gatcacggtt gtctgcaggc cttccttctg acgcgtcggc ggattctgag ggaactccac
     5521 gtaggactcg tagcgcccgg tcgccacgtt cagccggtag gcgcgcgcca ggaccgtgtc
     5581 ctggccggag gagctcacgc tgtcggggtc ggcgcgatag aggtttatgc agaggtagag
     5641 cacgggggac tgaaggtccg gaaggatgtt cacgatcgtc ggcaccaggg tgccgcttgc
     5701 ctgcaccggc agatgcgcct ggtccacttc ccgccgatag agcagctttc catccgacga
     5761 ataccagaac gtctcccagg acgcgggcag acgcgatacc acgaccaggt cgtcccggtt
     5821 cgtgacgtac agggcgtaga tgaaggcaaa gggggttccc ccgggtccct cctggccgat
     5881 gtaccccagg ggcttcccct tgcggtcgaa cctcaggatg acccggctct ggagcacgcc
     5941 ccgctccgtg tcctggaccg ctttcgcgtc cgcaaccgag tcctccacgt agagcgtctt
     6001 gtcgcttgac accgcaatct gaccgatgtc gttgaacggg taggccacgg cgccccgagt
     6061 gctggcctcc tccgccccgg tggtggggct tcccaggagc gtgggggtcg gattggtctg
     6121 ggggttgtag agcaggaaga gcagatctcc gtacgaggag aacaccatga ccttgcgggc
     6181 cggcccgttg gcgacgtaga accaaccgtc ccgcatcacc atgtggtttt ccccctcccg
     6241 ggcggcctcc cccggccgaa agaggtcgat ctggttatcg agccggccca ggttgaggct
     6301 gaagagaggc tcactgacga ggatctcgag ccccttgcgc ccgcaggcgg ccagcacgag
     6361 cagcaacggg atcaccaaga ggaaccggcg gagactacgg aagtgggaga ccatgggcgg
     6421 tggcatggta gccagcttcc cttgacctgt caatctcttg ctgaatacaa tggtcgcatg
     6481 cttcaaggcg ttctcacgtt ctttctcggc tccaagcacg agcatgacct gaaatccctg
     6541 gcaccctttg tccagcgcat cgatgcgctg gagctggagg tcatgggata cccggacgag
     6601 aagttcccgg ccaagaccgt ggagttccgc cagcggctgg cggcgggaga gcgcctggat
     6661 gacctgttgc cggaggcata cgccctggta cgggaggcgg cgcggcgccg tctgggggag
     6721 cggatcttcg acgtgcagat gatgggcgcc gtggtgctgc accagggaaa gatcacggag
     6781 atgaagaccg gcgaaggaaa gaccctggcc agcgtcccgg cggcctacct caacagcctc
     6841 accggcatgg gagtgcacgt catcacggtc aacgactacc tggcggagcg cgacgccgcc
     6901 tggatggggc ccatctacgg tctcctgggg gtcagcgtgg gggtgatcct cagccagatg
     6961 gacaacgacc agcgcaagat ctcctactcc aaggacatca cctacgggac caacaacgag
     7021 ttcgggttcg actacctgcg ggacaacatg tgctggagcc cgtcggacaa ggtccagcga
     7081 ggccacgcat acgccatcgt ggacgagatc gacaacatcc tgatcgacga ggcgcgcacg
     7141 ccgctcatca tctccggaat ggcggaggac gacaccttca agttcgcgga ggtgaaccgg
     7201 ctcgtggagc agctcgagga gtgcgagaag gatccggcca cgggcacgta tcccgacggc
     7261 gacgcccagc cgaagggaga cttcaagctg gaggagaaga acaagcgggt caccttcacc
     7321 gaccagggga tgaaccacat cgagagcctg ctgatgaagc gcggtctcat ccgcgactcc
     7381 ctgttcgtga gcgagaactt cgagtacatc cactacttca cccaggccct gcgggcccac
     7441 cggctctacg ccaaggacgt ggactacgtg gtccaggacg gcaaggtcca gatcgtggac
     7501 gagttcacgg gacgcatcct ctacggccgg cggtactcgg aagggcttca ccaggccatc
     7561 gaggccaagg aacggatcaa gatcgcggcg cgcaaccgga ccctggccac catcaccttc
     7621 cagaactact tccgcatgta cggcaagctg gcggggatga cgggcacggc ggacaccgag
     7681 gcccaggagt tcgccaagat ctacaacctg gacgtcgtgg tcatcccgac caaccgtccc
     7741 gtggtccgcg acgacgcgga cgacgagatc tacctgaccg aagaggagaa gttgacggcc
     7801 atctgcaacg ccatcgggga gctgcacgcc aagggccagc cggtgctcgt cggcaccatc
     7861 tccatcgaga agtcggagcg gctctcacgg atgctcaccg ggcgaggcat ccgccacgag
     7921 gtgctgaacg cgaagaacca cgcccgcgag gcgctcatca tcgccgaggc cgggacccgg
     7981 gggtcggtga ccatcgcgac caacatggcg ggacgcggga cggacatcaa gctgggcggg
     8041 aacccggagt tccgggcccg caagaaggca ggcacccagg cgacggagga cgtgtaccgc
     8101 cacgcgctca ccgaggagta cggccgctgg cagcaggact accagggggt gcggggcctg
     8161 ggcggcctgc acgtgctggg gaccgagcgc cacgaggccc gacgcatcga caaccagctc
     8221 cgcggaaggt ccggccgcca gggggacccc gggt
//
LOCUS       DASRZS010000220         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2050066, whole genome shotgun sequence.
ACCESSION   DASRZS010000220 DASRZS010000000
VERSION     DASRZS010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050066"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VFH83_05570"
     CDS             <1..514
                     /locus_tag="VFH83_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485865.1"
                     /translation="LAYQCYPDPDASRRDVLFQDGTVAFRWKDTASGLLLTALRAGRE
                     AETYNLVASSPTPYERICGISFSILKDPSDQDTVIGWLYLLKPSNPTGSTTIKVNREA
                     PMWVQPQGYKKELLDRDGKVAFRWVDRSFGIVMSRIHEGKVVARYNLVHIVYPDPEGG
                     LDIGPESPGE"
     gene            893..>2666
                     /locus_tag="VFH83_05575"
     CDS             893..>2666
                     /locus_tag="VFH83_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6485866.1"
                     /translation="MRNLKLLTKLLGGFVIVSLIILVVGYFGLNGARTLSTNAKTIGS
                     EILPKTNSLQDIKEAQTEFEAQENGLMGKNLDGAALMDAYSAIDAAKKRQDDAVKVYE
                     ALPRNKDENALWEQIVPALDTFWKAHLDFMAIEKEYELGRPPELAEKLATQSIEVEAP
                     QRNALSDLLDQALELDKKQAATAVSRAAATTGQVSLVAMVGLIAGPTIALALGILLAL
                     SIIRPLARGVTFAQVISGGDFTQRIDLRRKDEVGVLAGALNEVAAKLGGMVSTIRTNA
                     SQVASSSEQISVSSQRLSEGAQSQASTLEQTSAAVQELTASVDQVAEHARSQAAAVEE
                     GGATMSRVEASIKEVSESLNQISNLAGKSVEDAARGSAAVQDVVSGINLISASSEKIG
                     GIVSVISDIADQTNLLALNAAIEAARAGEHGRGFAVVADEVSKLADRSASSTKEIEAL
                     IRESVAHVAQGVETANGSRAAIGQIQEASRQVQEMIARLSRSMSTQMQAVRDLSRSLA
                     NVREMSQSISAATGEQTTNARQVATAVENVNDVTQGAASAAKEMSASTVQLASMAKDL
                     QGLMAQFKVDHSSGGEEADAAQVPA"
BASE COUNT          502 a          876 c          876 g          412 t
ORIGIN      
        1 cctggcgtac cagtgctatc cggatcctga cgcttccagg agggacgttc tcttccagga
       61 cggcacagtc gcgttccgct ggaaggatac cgcctccggg ctgctcctga ccgccctgcg
      121 agccggccgc gaagcggaga cttacaacct ggtcgcatcg tctccgaccc cgtacgagcg
      181 catctgtgga atctccttca gcatcctgaa agacccgtcg gaccaggaca cggtcatcgg
      241 gtggctctac ctgctgaagc cgtccaaccc cacggggtcg acgaccatca aggtgaaccg
      301 ggaggcgccc atgtgggtcc aaccgcaggg ctacaagaag gagcttctgg acagggacgg
      361 gaaagtggct tttcgctggg tggaccgttc cttcgggatc gtgatgtcga gaatccacga
      421 gggcaaggtg gttgcgcgat acaacctcgt acacatcgtc tacccggatc ccgaaggtgg
      481 cctggatatc ggtccagaaa gccccgggga gtagcagggc gatgaccgct tcccgcgtcc
      541 gtcacggccg ccgaacggcg gaagttccag ttgcgccaat tgttccaaaa tggtgcttgt
      601 acaggctcac acttggaacg gtggaacaag cgcgctcgtt cgccgaccgc cgctgagacc
      661 ctccacgcac ccagagttgc tcgtttttcc gccgcccccg cgctgaacgc gccggcgccc
      721 cttcccacgg ctttctcgtt gcgacttggc acgttgcgtg ctacgtcgaa ggggtcaccg
      781 ccgccagccc ggcggtcggc catgcgcgcg gcggatggtc gcaccctcac cccccagtgt
      841 ctgcgggagg cgcgtggccg ggtccatggc ggttgcgctc aggaggtccc gcgtgcggaa
      901 cttgaagctg ctcacgaagc ttctcggcgg tttcgttatc gtttccctga tcatcctggt
      961 ggtcggctac ttcgggctca acggcgcgcg gacgctttcc accaatgcga aaaccattgg
     1021 cagcgagatt ctccccaaga ccaacagtct ccaggacatc aaggaggcgc agaccgagtt
     1081 cgaggcgcag gagaacggcc tcatgggaaa gaacctggac ggggccgctc tgatggacgc
     1141 ctactccgcg atagatgccg ccaagaaacg acaggacgac gcggtaaaag tctacgaggc
     1201 gctgccgcgc aacaaggacg agaatgccct ctgggagcag atcgtcccgg ccctggacac
     1261 cttctggaag gcgcacctgg acttcatggc aatcgagaag gaatacgagc tgggtcggcc
     1321 gccggagctg gccgagaagc tcgccacaca gtccatcgag gtcgaggctc cccagcggaa
     1381 cgccctctcg gatctcctcg accaggcgct cgagctggac aagaagcagg ctgctaccgc
     1441 ggtctcccgc gccgcggcca cgacgggcca ggtctccctg gtggcgatgg tggggctcat
     1501 cgccgggccg accatcgcac tggccctggg cattctcctt gcgctctcga tcatccgccc
     1561 cctcgcccgg ggggtcacgt ttgcccaggt aatctccggt ggtgacttca cccagcgcat
     1621 cgacctgcgt cgaaaggacg aggtaggagt gctggccggc gccctcaacg aggtggcagc
     1681 gaagctcggg ggcatggtgt ccaccatccg cacgaacgcg tcccaagttg cctcctccag
     1741 cgagcagatc agcgtaagct cccagcgcct ctccgaaggc gcccagagcc aggcgtcgac
     1801 actggagcag acgagcgccg ccgtccaaga gctcacggcg tccgtggacc aggtggcgga
     1861 acacgcacgg tcccaggccg cggcggtgga ggagggaggc gcgaccatgt cccgggtaga
     1921 ggcctccatc aaggaagtgt cggagagcct gaaccagatc tcgaacctcg ccggcaagtc
     1981 cgtggaggac gcggcacggg ggagcgctgc cgtgcaggac gtggtctcgg ggatcaacct
     2041 gatctcggcg agctcagaga agatcggcgg catcgtcagc gtcatctcgg acattgcaga
     2101 ccagaccaac ctccttgcgc tcaatgctgc catcgaggct gcgcgggccg gagagcacgg
     2161 ccggggcttc gcggtggtgg cggacgaggt gagcaagctc gcagatcgaa gcgcctcgtc
     2221 caccaaggag atcgaggcgc tcatccgcga gagcgtggcg cacgtggccc agggagtgga
     2281 aaccgccaac gggtcgcggg cggccatcgg ccagatccag gaagcctccc gccaggtcca
     2341 ggaaatgatc gcacggcttt ccaggtcgat gtcgacacag atgcaggccg tgcgcgacct
     2401 gtcacggtcc cttgccaacg tgcgggagat gagccagagc atctcggccg ccaccgggga
     2461 gcagaccacc aacgcccgcc aggtcgcaac cgcggtggag aacgtcaacg acgtgacgca
     2521 gggcgccgcc tccgcggcga aggagatgtc ggcctccacc gtgcagctcg cctccatggc
     2581 aaaagacctg cagggactca tggcgcagtt caaggtggac cactcctccg gcggcgagga
     2641 ggcggacgcc gcgcaggttc ccgcgg
//
LOCUS       DASRZS010000221         4024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_512027, whole genome shotgun sequence.
ACCESSION   DASRZS010000221 DASRZS010000000
VERSION     DASRZS010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512027"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..3670)
                     /locus_tag="VFH83_05580"
     CDS             complement(242..3670)
                     /locus_tag="VFH83_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6485867.1"
                     /translation="MKTTRELQNRIFDRINPAVRFSVSRYVLAIGLFVAMLIFGGIAT
                     LSLGVDQFPSSSIPVAVVNTSYPGASPSVIDEQVTQVIESAVSTMGGITDISSTSSSG
                     SSMVMIDFDQSTDQVSDASQVASLVSAAAGRLPSGAGTPSVLTMNPNATPILQFGIAG
                     PGVSLAEVNDYTTTMLEPKLQRVSGVANVSLSGAPSREFQVLLDPSRLQAYGLGATAV
                     VNAIASSSVTSPIGTITTSKNALTFSTENVPSDKEAVESIVVDTTSGVRVRDLANVRD
                     RATSSTYTRINGKPVVLVSIEQTATSNAVAVVSDVRRILSQVVLPTGYEIIYSNDTTA
                     PIQASIHSTYRELILTTIVVALIVLLFLGKLNTAVSVILAIPIALSASPVLYRLCGFT
                     LNLVSMLALIVAIGIVVDDSIVVAENVERYRAMGYHPRESVLKGASEIFSAVVAATLS
                     ILAVLLPVSFLGGMIGSFLRQFCLGLAAAVLFSLLEAVLFLTVRLAYLTDVGRRRDWG
                     ELRRSFTRPGEAARWGLVAWRRPVGIVVAVGLAAACLLTHHLLLLPCLLLYPAALMLL
                     RYLGLVLLCLFEALSMTLHGWTEAGLGWVREAYARSLGGLLRRGVVVLLVTLTVVAGI
                     LVFLAPRLTFSFLPQTDNGTLSLNGSMRRGATLSVTNAAAASLERYLLSQPEVRVVQT
                     TVGGGGMFGGGSSYENFSMRVTLVDVGKRAGTFTLIARYRIRLNQILQAQDPSASVMM
                     MAGGGFGGGGSALSLALLSSDREALSARSARVVEAIKSNPYVQDVTSSVSETTVERQF
                     IPDPVRLQDAGVTAGDVATLLQTYATGVTAANVEVGGLSYPITVRIDPLFLSDGQALL
                     NLPLYSPKLQATVPVGQLGSVVLRESPVSIRRTNRLYSASLSINLKQGAPTALSFQNQ
                     LAQDLTAQGLLDTTLTLGSGSLMGAGALAQQMSSTAIWAFLLAFFLAYLVMAAQFNSW
                     RYPVYLLLPVPLAIVGALAFAIVLGGGLDIFGVLGMLLLIGLSAKNAILYLDFVVERL
                     DKMPLLEALVDSARLRFRPIIMTTLTVLVISGPLVFAQGQGSEFGQKLGVVMFGGILS
                     SAILTFFVVPTAFYVFERKRTRPVQASDLPDRTETRQEDRAPAPVPWAPGSKAT"
     gene            complement(3667..>4024)
                     /locus_tag="VFH83_05585"
     CDS             complement(3667..>4024)
                     /locus_tag="VFH83_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485868.1"
                     /translation="TSPSVPVNGVVQMVAAVPSSLPLPFGSIGSVCYSVMRASGSLVP
                     AASLLSNETTNFVWVVVDNAVAMRPVSLLAESGDMAAVSGLGAGSQVVLNPAPGLLDG
                     TRVRIVTPDSAKAGSR"
BASE COUNT          756 a         1252 c         1418 g          598 t
ORIGIN      
        1 agcggcgcgg gaccgactcc gccgacgtgc ctcgccgtcg cggtatcccg ggagcaacac
       61 accataggct cccggatggt ggcggacttc ctccggctcg acgggtggga cgttgtgttc
      121 ccacggggaa acctcggcat ccgtcacgtg atcgagatgc tgagcaccga cctccgaacc
      181 ttttggcgct gtccgtcact ctggaggaga acgtcgcgga ggaggcgaac ctgacctcga
      241 ctcaggtggc tttcgaccct ggcgcccacg gcaccggggc aggtgcccga tcctcctggc
      301 gcgtctccgt ccgatcgggg aggtcagagg cctgcacggg tcgcgtccgc ttgcgctcga
      361 acacgtagaa ggcggtcggc accacgaaga aggtcaggat cgcggaggaa aggatccctc
      421 caaacatcac aacgccgagc ttctgaccga actcggagcc ctgaccctgc gcgaagacca
      481 gggggcccga gatcaccagt accgtcaggg tggtcatgat gatgggacgg aagcgaagcc
      541 gggccgagtc gacgagggcc tccaggagcg gcatcttgtc caggcgttcg accacgaagt
      601 ccaggtagag gatggcgttc ttcgcggaca gaccgatgag gagcagcatt ccgagcacgc
      661 cgaagatgtc cagaccgccg cccagcacga tggcgaacgc cagcgccccg acgattgcca
      721 gcgggacggg caggagcaga tagaccggat agcgccagga gttgaactgg gccgccatga
      781 cgagataggc gaggaagaag gccaggagga atgcccagat cgccgttgag gacatctgct
      841 gcgccagcgc tcccgcgccc atcaagctgc ccgagccgag ggtcagcgtg gtgtccagca
      901 gcccctgggc cgtcaggtcc tgggcgagct ggttctggaa gctcagggcg gttggtgccc
      961 cttgcttcag gttgatggac aacgaggcgc tgtacaggcg attggttcgc cggatgctga
     1021 ccggcgactc ccgaaggacg acgctcccaa gctgcccgac gggtacggtg gcctggagct
     1081 taggggagta cagcggcagg ttgagcagcg cctggccgtc ggagaggaag agcgggtcga
     1141 tcctgaccgt gatcggatag gaaagcccgc cgacctccac gttggcggcc gtaacgccgg
     1201 tggcgtatgt ctgcaggagg gtggcaacgt cgccggcggt gactcccgca tcctgcaggc
     1261 ggaccggatc gggaatgaac tgccgttcga ccgtcgtctc agagacgctg gaggtgacgt
     1321 cctggacgta gggattgctc ttgatggctt cgaccaccct ggcgcttcgg gcggacaacg
     1381 cctccctgtc cgaggaaagc agagccaggg aaagagcgga gcccccgccg ccgaatcctc
     1441 ctcccgccat catcatgacg gaggcggatg ggtcctgggc ctggaggatc tggttcaacc
     1501 ggatccggta gcgcgcaatg agggtgaagg tgccggcccg cttgcctacg tctaccaggg
     1561 tgacgcgcat ggagaagttc tcgtaggagc ttccgccccc gaacatcccg ccgccgccga
     1621 ccgtggtctg caccacccgc acctccggtt ggctcaggag gtaccgctcg agggaggcgg
     1681 ccgcggcgtt ggtcacggag agcgtggcgc cccggcgcat gctcccgttg aggctcaggg
     1741 tgccgttgtc cgtctgcggc aggaagctga aggtgagccg cggcgcgagg aagacgagga
     1801 tccccgccac cacggtgagc gtcaccagga ggacaaccac cccgcgcctc aggagcccgc
     1861 caagggacct cgcgtaggcc tcccgtaccc atcccaaccc cgcctcggtc cacccgtgga
     1921 gcgtcatcga aagtgcctcg aagaggcaca gcaggacgag gccgaggtaa cgcagaagca
     1981 tcagcgcggc gggatagagc agcagacaag gcaggagcag aaggtgatgg gtcagaaggc
     2041 aggccgcggc cagccccacc gccacaacga tcccgaccgg cctgcgccac gcaaccaggc
     2101 cccagcgggc ggcctcgcca gggcgcgtga agctgcggcg tagctcgccc cagtcgcggc
     2161 gccgcccgac atcggtgagg taggccagac gcacggtgag gaacagaaca gcctcgagga
     2221 gtgagaacag gaccgccgcg gccagtccaa ggcagaactg gcgcagaaaa ctgccgatca
     2281 tgccgcccag gaagctcacc ggcaggagca cggccaggat cgacagggtt gccgccacga
     2341 cggcgctgaa gatctcactg gcacccttga ggacggattc tcgcggatgg tagcccatgg
     2401 cgcggtagcg ctcgacgttt tcggccacga cgatcgagtc atccaccacg atgccgatgg
     2461 ccacgatcag ggccagcatg gagaccaggt taagggtgaa accgcacagc cggtacagga
     2521 cgggagaagc cgagagggca atcgggatcg caaggatgac cgacacggcg gtgttcagct
     2581 tcccgagaaa caggaggacg atcagcgcca ccacgattgt cgtgaggatc agctcccggt
     2641 aggtgctgtg gatggatgcc tggatgggag cggtggtgtc gttgctgtag atgatctcgt
     2701 agccggtggg caggaccacc tggctcagga tccgccgcac gtcggacacg accgccacgg
     2761 cgttggaggt cgcggtctgc tcgatggaga ccaggacgac cggcttcccg ttgatccggg
     2821 tgtaggtgga ggaggtcgcg cggtcccgca cgttcgccag atcgcggacc cggaccccgg
     2881 aggtcgtgtc cactacgata gactccaccg cctccttgtc ggatgggacg ttctccgtag
     2941 agaaggtcag cgcgttcttg ctggtcgtga tggtgccgat ggggctggtc accgaggagg
     3001 aggcgatggc gttcactacc gcggtggcgc cgagcccgta cgcctggagg cggctgggat
     3061 cgaggagaac ctggaactcc cttgaggggg cgccgctcag cgacacgttg gccacgcccg
     3121 agacgcgctg gagcttgggc tcgagcatcg tggtggtgta gtcgttgacc tccgcgaggc
     3181 tcactccggg tccggcaatc ccaaactgga ggatcggcgt ggcgttcggg ttcatggtca
     3241 ggacggaggg cgtcccggct cccgagggca gccgtcccgc tgccgcggaa accagggatg
     3301 ccacctggct ggcatcgctt acttggtcgg tggactggtc gaaatcgatc atcaccatgc
     3361 tggacccgct ggagctcgtg gagctgatgt ccgtgattcc acccatggtg gagacggcgc
     3421 tctcgataac ctgggtcacc tgctcgtcga tcacgctcgg tgaggcgccc gggtaggacg
     3481 tgttcaccac ggcgacagga atgctggacg atgggaactg gtctacgccg agactcaggg
     3541 tggcgattcc cccgaagatg agcattgcga cgaacagccc gatggccagc acgtagcggg
     3601 agacggagaa gcgcactgcg ggattgatgc ggtcgaagat ccggttctgc agctctcgcg
     3661 tcgtcttcat cgggagcctg ccttcgcgga gtctggggtg acgatcctga cccgggtgcc
     3721 gtccaggagt ccgggcgccg ggttgaggac cacctgcgag ccggccccaa gccccgacac
     3781 cgcggccatg tctccgctct ccgcgaggag actcaccggc cgcatggcca cggcgttgtc
     3841 cacgacgacc caaacgaagt tggtggtctc gttgctcagg agcgacgcgg ccggcaccag
     3901 ggagccgctg gcgcgcatga cggagtagca gaccgacccg atggacccga aggggagcgg
     3961 gagcgagctc ggaacggcgg ccaccatctg caccactccg ttcacgggaa cgctcggcga
     4021 ggtg
//
LOCUS       DASRZS010000222         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1279629, whole genome shotgun sequence.
ACCESSION   DASRZS010000222 DASRZS010000000
VERSION     DASRZS010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279629"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VFH83_05590"
     CDS             <1..654
                     /locus_tag="VFH83_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485869.1"
                     /translation="SLSRSTSTVQLGGLTTSLVAQQMLPVSPIGTVLGTAQEFLPSTL
                     QVSYVTGGQPLWFWKDRIRFNPSINSSWNLNLQQFTNNSFTFSLNLDLSIYKFLELTF
                     SSVSNNTKTYRYFPALANQVNEQWVNPITDLLDSFDFFNDQVRSESAFKIRSLAFKII
                     HHLQDWDVTVQYQGTPTLSTVTGKYQWSPSFAINVAWVAVSQVNSNIKGDYTGVTLR"
     gene            698..1657
                     /locus_tag="VFH83_05595"
     CDS             698..1657
                     /locus_tag="VFH83_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET6485870.1"
                     /translation="MERPRTVVVSDETILPEICGANDSNLKVFEEILGVRVFSRGNEI
                     LLDTVDDSVERTFTLLLEQLEDHIRLGQLPGPDLIRSIHGSLASGDTDRTELLKSTLV
                     VVPGGQRKVYPRTLNQAHYLHAIETGELTFGVGPAGTGKTYLAVALALKQVLGQQRRK
                     LIITRPVVEAGESLGYLPGDLTQKIHPYLRPLYDAIESIVPMETFRRMEDNGTIEIAP
                     LAYMRGRTLADAFIVLDEAQNTTREQMKMFLTRIGENSRTVITGDITQIDLPRKSESG
                     LLTVIPILKPIREIRFVFFEERDVVRNALVRKIVHAYETAHEE"
     gene            1647..>2040
                     /locus_tag="VFH83_05600"
     CDS             1647..>2040
                     /locus_tag="VFH83_05600"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485871.1"
                     /translation="MKSSRFRLLGRLGEQVRRLRLERWTVISVTVAFAAALAIVLIST
                     QSQSFSLGRGAASWEAGAVADMDYVVERDFQYVDDKATQAKRDAREKLVPPVYTINSV
                     VTAATLDTFDRFTQTVTRLIRQGTTEDKL"
BASE COUNT          403 a          684 c          595 g          358 t
ORIGIN      
        1 tcgctctccc gctccaccag caccgtgcag ctaggcggtc tgaccacgtc gctcgttgcc
       61 cagcagatgc tgcccgtcag ccccatcggg acggtcctgg gcacggcgca ggagttcctg
      121 ccatccacgc tgcaggtaag ctacgtcact ggcggacaac ccctgtggtt ctggaaggat
      181 cgaatccggt tcaacccctc cataaacagc tcctggaacc tgaacctcca gcaatttacc
      241 aacaactcgt tcaccttctc cttgaacctc gacctgtcca tctacaagtt cctcgagctc
      301 accttctcct ccgtgagcaa caacaccaag acctaccggt actttccggc gcttgcgaac
      361 caggtcaacg agcagtgggt gaaccccatt accgacctcc tggactcctt cgacttcttc
      421 aacgaccagg tgcggagcga gtcagccttc aagatccgca gcctcgcctt caagatcatc
      481 caccacctcc aggactggga cgtcaccgtc cagtaccagg ggacccccac cttgagcacc
      541 gtgaccggca agtaccagtg gtcccctagc ttcgccatca acgtggcatg ggtcgctgta
      601 tcccaggtaa acagcaacat caagggcgac tacaccggcg tcacgctgcg ttgacgcgcc
      661 ccggctattt ccactacgat taggggcagg aagctgaatg gaaagaccac gaacggtcgt
      721 tgtcagtgac gaaaccatcc ttcccgagat ctgcggggca aacgacagca acctgaaggt
      781 tttcgaagag atcctgggcg tccgggtttt ctcccggggc aacgagatcc tgctggacac
      841 ggtcgacgat tccgtggagc gcacctttac gctgctcctg gagcagcttg aagaccacat
      901 tcggctgggt cagctccccg ggccagacct gatccgctcc atccacggct cgctggccag
      961 cggcgacacg gatcgtacgg agctcttgaa gagcaccctg gtggtggttc cgggcggcca
     1021 gaggaaggtc tacccgcgta cgctgaacca ggcgcactac ctccacgcca tagagacggg
     1081 ggagctcacc ttcggagtgg gtccggccgg cacagggaag acctacctgg cggtggcgct
     1141 ggccctcaag caggtcctgg gccagcagcg gcgcaagctc atcatcaccc gcccggtggt
     1201 ggaggctggg gagagcctgg gctaccttcc gggggatctg acccagaaga tccaccccta
     1261 ccttcggccc ctctacgatg ccatcgagtc catcgtgccg atggagacct tccgtcggat
     1321 ggaagacaac gggaccatcg agatcgcgcc cctggcgtac atgagagggc gcaccctcgc
     1381 ggacgccttc atcgttctgg acgaggccca gaacacgacc cgggagcaga tgaaaatgtt
     1441 cctgacccgc attggcgaga actcgcggac cgtcatcacg ggggatatca cccagatcga
     1501 cctgccccgg aagagcgagt cgggcctgct caccgtgatc cccatcctga agccgattcg
     1561 cgagatccgc ttcgtattct tcgaggagcg ggacgtggtg cgcaacgcgc tggtgcgaaa
     1621 gatcgtccac gcctacgaaa ccgcgcatga agagtagccg cttccgtctg ctcggccgtc
     1681 tgggcgagca ggtgagacgc ctgcgcctgg agcggtggac cgtcatcagc gtgacggtgg
     1741 cgtttgccgc cgccctggcc atcgtgctca tcagcaccca gtcccagagc ttctccctgg
     1801 gtcgaggcgc cgcctcctgg gaggcgggcg cggtggcgga tatggactac gtcgtcgagc
     1861 gcgatttcca gtacgtcgat gacaaggcca cgcaggcgaa gcgcgacgcc cgggagaagc
     1921 tggtgcctcc cgtgtacacg atcaactcgg tcgttaccgc cgccacgctc gataccttcg
     1981 accggttcac gcagaccgtg actcggctga tccgccaggg gaccaccgag gacaagctgt
//
LOCUS       DASRZS010000223         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_125991, whole genome shotgun sequence.
ACCESSION   DASRZS010000223 DASRZS010000000
VERSION     DASRZS010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125991"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFH83_05605"
     CDS             complement(<1..270)
                     /locus_tag="VFH83_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HET6485872.1"
                     /translation="MKVNAKYVNPFIDAAMRVVTQIAGIEVRRGHLSYKSKVEPSFGV
                     SIIVGIYGYLVGQVVYSLDTNLATRLVDKLLTGKSPQEKNILFVDT"
     gene            complement(272..634)
                     /locus_tag="VFH83_05610"
     CDS             complement(272..634)
                     /locus_tag="VFH83_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006837510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6485873.1"
                     /translation="MPKRIMIVDDANFMRMIVKDTLVPQGFEICGEAANGEEAVKLYQ
                     KLKPDLVTMDITMKQKNGLEAAQEILAKDPQARIVMVTALGQEKMLMDSLAIGVKDFV
                     VKPFEAHRIISAVQKALA"
     gene            970..1719
                     /locus_tag="VFH83_05615"
     CDS             970..1719
                     /locus_tag="VFH83_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485874.1"
                     /translation="MDESTTGPGQALRSDRLNLFETIIPGLAHEINNCNQAILLTAEV
                     LHGVSRSLQRIADRYLEDHGDFVAGGVQYSSIREELQGHFEDIITAAQSIRRLVTDLR
                     AFTGGGHRATDPVNLNRVLENARTLLTNTIRKSTDAFSLELGTEMPDISVDFAWLLQM
                     VLLLILNACRGHADRRDPLGIVTRWSSESRKAVCEVTYHGAESTTESRDAAWATVSWM
                     SEQIGADFLLARDAEGITRITLRIPSERSGG"
     gene            1724..>2674
                     /locus_tag="VFH83_05620"
     CDS             1724..>2674
                     /locus_tag="VFH83_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6485875.1"
                     /translation="MARQNPAKRPVLIVDDDRAVLLNVRMALESGAIGNLTTCDDSRE
                     VLTILSRQEIDLLLLDLNMPHRSGTDLLPEIREQYPDLPVIIVTGSTEVGLAVDCMKL
                     GVFDYLVKPIERSKLLATVGRALEIRELRRENTSLKSRLIDGKLDHPEAFGPIVFRDE
                     KMRSVLMYVESIAESRQTVLITGETGVGKELIATAIHALSGRKGPLVTVNVAGYDESM
                     FSDALFGHLRGAYTGADKDRSGLIERSEGGTLFLDEIGDLKPESQVKLLRLLESREYY
                     PLGSDAPRRTDARIVVATNRDLQRSVDDGAFRKDLYFRLET"
BASE COUNT          509 a          830 c          845 g          490 t
ORIGIN      
        1 ggtgtccacg aacaggatgt tcttctcctg gggagacttg ccggtgagca gcttgtcgac
       61 cagccgggtg gccaggttcg tgtccaggct gtacaccacc tggccgacca ggtagccgta
      121 gatcccgacg atgatggaga cgccaaagga gggctccacc ttcgacttgt aagagaggtg
      181 accgcgtcgc acctcgatcc cggcgatctg agtaaccacc cgcatggcgg catcgatgaa
      241 cgggttcacg tacttggcgt tgactttcat gctatgccag ggctttctgg accgcggaga
      301 tgatccgatg cgcttcgaag ggcttcacca cgaagtcctt cacgccaatg gccagggaat
      361 ccatcagcat cttctcctgt cccagcgccg taaccatgac gatgcgggcc tgggggtctt
      421 tggccaggat ctcctgggcc gcctccagtc cgttcttctg tttcatcgtg atgtccatgg
      481 tcaccaggtc cggcttcagc ttctggtaga gcttcacggc ctcctcaccg ttggccgcct
      541 caccgcagat ctcgaacccc tgcggcacca gggtgtcctt cacgatcatc cgcatgaagt
      601 tcgcgtcgtc gacgatcatg atccgttttg gcatcgcgtt cctcctcgcg ctccggcacg
      661 gcacaaacga ttggaccgcc gctcttcctt tccgcggcgg tcccccatgg tctgcaagct
      721 gcatgccaga ccggcggtag gctgctaagt gtttgagagg gaaggaattg atgcgctgtc
      781 gctgccgagg ccgtttcttt tcaggaagta aaggaacatt gcagaccttt ttctgcggct
      841 gggatatctt cttttctcgt atgcacttaa ctccggctca aggtaagttt aggtgtatac
      901 gatgcggggc cgcctgccgc aaaggccacg ctcgaactcg gaacccgaat cgaggatcag
      961 actagtatca tggatgagag cactactggc cccgggcagg cactgcgcag cgaccggctc
     1021 aatctcttcg agacgatcat ccccggcctg gcccacgaga tcaacaactg caaccaggcc
     1081 atcctgctca cggcggaggt gctccacggc gtgagccgaa gcctgcagcg gatcgccgac
     1141 cgctacctgg aggaccacgg ggatttcgtg gccggggggg tgcagtactc atccatccgt
     1201 gaggagctgc aagggcattt cgaagacatc atcaccgcgg cgcaatccat ccgacggctc
     1261 gtcaccgatc tgcgcgcctt cacgggaggc ggccataggg ccacggaccc cgtcaacctg
     1321 aaccgggtcc tggagaacgc ccggacgctc ctcaccaata ccatccgcaa gagcacggac
     1381 gccttctctc tggagttggg cacggagatg ccggacatct ccgtggattt cgcctggctg
     1441 ctgcagatgg ttctcctcct gatcctgaat gcctgtcggg gacacgcaga ccggcgggat
     1501 ccgctgggca tcgtgacgcg gtggagctcc gagtcgcgca aggctgtctg tgaggtgacc
     1561 tatcacggcg ccgagtcgac gacggagagc cgggatgcag cgtgggcaac cgtatcctgg
     1621 atgagtgaac agattggagc ggacttcctg ctggcgcggg acgcggaggg aatcacccgg
     1681 atcaccctcc gcataccgtc ggagaggtcc ggggggtagg cggatggcac gacagaaccc
     1741 ggcgaagcgc cccgtcctta tcgtggatga cgatcgggcc gttcttctca acgtacggat
     1801 ggccctggag tccggcgcaa tcggcaacct caccacctgc gacgactcgc gagaggtgtt
     1861 gacgatcctg agccgccagg agatcgacct gctccttctc gacctgaaca tgccccatcg
     1921 gtcggggacg gacctcctgc cggagatccg agagcagtac ccggacctgc cagtgatcat
     1981 cgtcaccggg agcaccgagg taggcctggc cgtggactgc atgaagctcg gggtcttcga
     2041 ctacctggtg aagcccatcg agaggagcaa gctcctggca acggtcggcc gtgccctgga
     2101 gatccgggag ctgcggcggg aaaacacctc gctcaagagc cgcctgatcg acggaaagct
     2161 cgaccacccg gaggcgttcg ggccgatcgt ctttcgggac gagaagatgc gatccgtgct
     2221 catgtacgtc gagtcgatcg ccgagagccg ccagacggtc ctcatcaccg gagagaccgg
     2281 tgtgggaaag gagctgatcg caacggccat ccacgcgctc agcgggagaa agggcccgtt
     2341 ggtgacggtg aacgtcgccg gctacgacga gagcatgttc tccgacgcgt tgttcggaca
     2401 cctgaggggc gcgtacaccg gcgcggacaa ggaccgcagc ggtctgatcg agaggtcgga
     2461 aggcgggacg ctctttctgg acgagatcgg ggacctcaaa ccggaatccc aggtcaagct
     2521 cctgcggctc ctggagagcc gggagtacta cccgctgggc tcggacgcgc cgagacgcac
     2581 cgacgcccgc atcgtggtgg ccaccaaccg ggacttgcag aggtccgtcg acgacggagc
     2641 gttccgcaag gacctgtact tccgactgga gacg
//
LOCUS       DASRZS010000224        11687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2433817, whole genome shotgun sequence.
ACCESSION   DASRZS010000224 DASRZS010000000
VERSION     DASRZS010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11687
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2433817"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFH83_05625"
     CDS             <1..444
                     /locus_tag="VFH83_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HET6485876.1"
                     /translation="FFITHGFKENAEAQEKRLLAGSLSDISKILYLEIIDTTFSIDGV
                     LGAFAFTLSVPLIIAGNGLGAFLVREITIGNLARIKRYHYLKNGAMYSILLLGTVMMC
                     DAFGLETPPWVSPAATFGIVAFFFLRSVQEARRGAKPVARDREGG"
     gene            445..1212
                     /locus_tag="VFH83_05630"
     CDS             445..1212
                     /locus_tag="VFH83_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HET6485877.1"
                     /translation="MEEVREVIARGRSFLRANWAQFTHVDSDQERGVPAPPLQKPLPP
                     DAQVVTLPDPGSLALGSMPVSEAIRNRKSRRKYSAEFLSREELSFLLWATQGVRRRSP
                     INTLRTVPSGGARHALETYLYLTRVAGIGEGLYRYLPLDHALLVLPGPEGLAGRLDEA
                     LFEQSWDAAAVFVWAAVPYRMEWRYSWVSHKIIALDAGHVCQNLYLACEALGCGTCAI
                     GAYDQEKLDALLGLDATEEFSVYAAPVGRQIDRAATP"
     gene            1621..1914
                     /locus_tag="VFH83_05635"
     CDS             1621..1914
                     /locus_tag="VFH83_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009109279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET6485878.1"
                     /translation="MAKKIYVGNMNYNTSEDTLRQLFAQYGEVMSVNVITDRYTGRAK
                     GFGFVEMASEDAARAAMTALNGKEVDGRQLKVNEANEKPRDASRGGYGGGRGY"
     gene            complement(2022..3770)
                     /locus_tag="VFH83_05640"
     CDS             complement(2022..3770)
                     /locus_tag="VFH83_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HET6485879.1"
                     /translation="MTDSAVSASGLDRDAPKSDFIRDAVQEDLRSGRFDRVRTRWPPE
                     PNGYLHIGHAKAICIDFGIAESFGGKCNLRFDDTNPTKEEQEYVDGIKEDTRWLGFDW
                     EDREFFASDYFEQLYQWAEKLIRDGKAYVCDLSEQEIREYRGTDVTDKGPVTVTPPGR
                     DSPYRNRSPEESLDLFRRMRAGEFPDGARTLRARIDMAHPNLHMRDPVMYRIRHAQHH
                     RTGDAWCIYPMYDYQHPLSDSIEGVTHSLCSLEYEIHRPLYDWFLENLGIFHSRQIEF
                     ARLNVTHTVLSKRWLLELVKDGRVRGWDDPRMPTIAGLRRRGYTPEAIRDFVSRVGTA
                     KTSSTVEVALLEHCLREDLNRRSPRVMAVLRPVKLVIENWPSGQVEEIEAVNNPEDPA
                     AGTRKVAFTGTLYIESDDFREVPPPKYYRLSPGQQVRLRYAYIIRCTGLRKDPTTGAV
                     EEIRCTYDPATRGGNAPDNRSVKATIHWVSAEHALSAEARLYDHLFSAEYPQEVPPGV
                     DWKDTLNPGSLEVISDCKVERSLSAARIGERFQFERLGYFCVDPDSSERLVFNRTISL
                     KDTWAKIEKKAGLKGS"
     gene            complement(3882..4289)
                     /locus_tag="VFH83_05645"
     CDS             complement(3882..4289)
                     /locus_tag="VFH83_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET6485880.1"
                     /translation="MVMGQIPEPVLSALLDALPIEFSIVDGNDKVMAWNRHETRLFRR
                     VETVLGRDVRQCHPSKSLKMVEQIISEMRQGSRDSAEFWLDMRLPDQEQPEKVLIQYF
                     ALRDSTGRYLGCLECSQKLNRLQRIQGQKRLLD"
     gene            complement(4534..5175)
                     /locus_tag="VFH83_05650"
     CDS             complement(4534..5175)
                     /locus_tag="VFH83_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485881.1"
                     /translation="MRSPKKYLDVFVLSGLLTSFFYGFLNPLYVSVILSRVDGRIIAL
                     GSFLASGLPVLIGAAMANPRVFRRLYAVLPVIMAIELAASAASVLVAAVNVMAYYLAS
                     MFILGTVGTSVVYLLQKEKEVRYRRGRAAFDRRCAMADSIGSLGGSAVSVATVVALRD
                     PLVVAVLGAVQTLVVYGVFLLLYRKVPARKARSGDLEGHPWNPAFADPQGLAA"
     gene            complement(5385..6038)
                     /locus_tag="VFH83_05655"
     CDS             complement(5385..6038)
                     /locus_tag="VFH83_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET6485882.1"
                     /translation="MEQDLLHLIDAGRRDAVADLLESEPATASIRAPDGVSPVLYALY
                     RGQRDIADLLLTAGAPVDVFVASALGLDGRLERILADRRDLATAYSPDGWTPLHLAAF
                     FGSERAAGRLLEAGADPSAVAHNAQANHPLHSACAGRRPPVAILLLDHGAEPDYQVAG
                     VTPLMIAAANGLAVVVTRLLQAGADLSRRSPQGKTAMDYAVSGGWLDVQQILRRGRS"
     gene            6158..6922
                     /locus_tag="VFH83_05660"
     CDS             6158..6922
                     /locus_tag="VFH83_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HET6485883.1"
                     /translation="MTPKKNYLIDMDGVLVRGSTPVPGADGFLGRLKERALEYLVLTN
                     NPQYTQNDLSYRLGSVGLPIEPRRIFTSAMATASFLKSQESNRRVFVIGESGLTGPLH
                     EAGFIITDIDPAFVVLGETPSLSYQQITAAIRLISSGVRFIATNPDANGPGEGGTVPA
                     CGALAALIEKASGVSPFFVGKPNPLMMRTALNYLGVHSEHTVMIGDRMDTDIVAGVES
                     GMETILVLSGVTARSEVARFPYRPASIQDSVADIEV"
     gene            complement(6958..7842)
                     /gene="folD"
                     /locus_tag="VFH83_05665"
     CDS             complement(6958..7842)
                     /gene="folD"
                     /locus_tag="VFH83_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HET6485884.1"
                     /translation="MAARIIDGTEISGRIRAEVQSGVEALKKERGITPGLAVVLVGDN
                     PASKSYVKGKNKAAHEIGIYSEQHNLAATTTESELLAVVDRLNHDPKIHGILVQLPLP
                     DHIDEQKIIAAIVPDKDVDGFHPVSLGRIMTGTPGFRPCTPLGIQKLLSYSGVTVAGS
                     HVVVVGRSNIVGKPIANMLMQKEADADATVTVCHSRTRDLPSVTRLADILIAAIGRPE
                     FVTADMVKEGAVVIDVGINRIEDPTHPKGQRMVGDVKFDEVSRKASAITPVPGGVGPM
                     TITMLLYNTLQSAKAGKA"
     gene            complement(7857..8660)
                     /locus_tag="VFH83_05670"
     CDS             complement(7857..8660)
                     /locus_tag="VFH83_05670"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET6485885.1"
                     /translation="MLIIGELINSTRKQIRAAVEKADGAYLQDLAKRQAAAGANWIDV
                     NAGAFPNDEVEKMRWLITTIREVTDAPLSVDSPRAAAVEAGLAMAGKEPFLNSISAES
                     DRYKTLIPLVKKYGARVVALSLDDSGMSDDFDKVWQVADGLIRRLEDDGVPPDHIFVD
                     PLIRPVSTNGEYGMGALSIVERITAKHPQVHKTCGLSNVSYGLPKRKLVNQVFMAMAI
                     AKGMDSAIIDPLDARMMATVAAAEALAGKDQFCMNYITAEREGKLEGMA"
     gene            8798..9961
                     /locus_tag="VFH83_05675"
     CDS             8798..9961
                     /locus_tag="VFH83_05675"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET6485886.1"
                     /translation="MENFTFQNPTRILFGRGAELQAGAEAARHGSRVLLHYGAGSIKA
                     SGLYDRVCASLRDAKAPFVELGGVKPNPRLSLVREGIRLCREQKLDFILAVGGGSVID
                     SAKAIAVGVPYTGDVWDFYTGKAKVERSLPVGVVLTIPAAGSESSNGSVVTNDDGALK
                     RDTGAECMRPVFAALNPELTFTLPPYQTACGAADIMAHVMERYFTPAKGVDFTDRLCE
                     ATLRTIIDTVPRALAEPRDYDARANILWASTIAHNDLLGTGRVGDWASHMIEHELSGL
                     YDVAHGAGLAVVFPAWMRHVYRNDTGRFARFACQVWGVEPCFEDPERTALEGIRRTAA
                     FFHQIGLPTTLKELGVPQARLAEMAHKAVWRGPLGNFRKLDERDVLEVYRLAG"
     gene            complement(9981..11228)
                     /locus_tag="VFH83_05680"
     CDS             complement(9981..11228)
                     /locus_tag="VFH83_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET6485887.1"
                     /translation="MYERNVAHIEELVREQNDWRGHCINLIASENVTSRRVRGVMGSD
                     FSHRYAEGHPGERYYQGTEIIDEIESLLKQHMKALFRCKHSDVRPISGTIANDAVFSR
                     YIRPGDVAMVNSTPGGGHISHHRAGSVGKYTKNIVSFPLTADGYHIDVEKTLDLMKAL
                     EPKVLIMGKSLFLFPEPVKEIAPVCKRRGITLIYDGAHVLGLIAGHQFQDPLGEGAPL
                     VTASTHKTFFGSQRGLILANVDDEEWRRIDKGAFPGSSSNHHLETLVTMTMATYEMLE
                     FGVEYARQTVANAKALARKLHQLGFRVQGEQFDFTETHQVAVDMGDFGGGDTAARDLK
                     DNHLIVNMNLLPFETLDKATAPAGIRMGVQEMTRVGMREPEMEHIAELFKKCLVDKKF
                     VGDEVKEMRSKYQEVCYSFDGKE"
     gene            complement(11250..>11687)
                     /locus_tag="VFH83_05685"
     CDS             complement(11250..>11687)
                     /locus_tag="VFH83_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET6485888.1"
                     /translation="TRVAAMGGSQGGGLTLACASLEPRIAKAMPVFPFLCDYRRVWEM
                     DQAKRAYEELAWYFRAFDPQHRREEEIFTRLGYIDAQYLAPRIKAEVVMATGLMDEVC
                     PPSTQFAAYNRMTCKKRTIIYPDFAHESITGVEDEVFEFLAGL"
BASE COUNT         1999 a         3750 c         3776 g         2162 t
ORIGIN      
        1 ttcttcatca cgcacgggtt caaggagaac gcggaagcgc aggagaaaag gctcctggcg
       61 ggaagcctgt cggacatcag caagatcctc tacctggaga tcatcgatac gaccttctcc
      121 atcgacggcg tgctgggggc attcgccttc accctgtcgg tgcctctgat catcgctggg
      181 aacggtttgg gcgcgttcct ggtccgggag atcaccatcg gaaacctggc ccgcatcaag
      241 cggtatcact acctcaaaaa cggcgcgatg tactcgatcc tgctgctggg gaccgtgatg
      301 atgtgcgacg ccttcgggct ggagacgccg ccgtgggtct cgccggccgc gacctttggc
      361 atcgtggcgt tcttcttcct tcgatcggtt caggaggcgc gcaggggagc gaaacccgtt
      421 gcgcgagaca gggagggtgg atagatggag gaggttcggg aggtcatcgc gcgggggcgc
      481 tcgttcctcc gggcgaactg ggcccagttc acgcacgtag attcggacca ggagagaggg
      541 gtgccggctc cgccacttca gaagcccctg ccgccggacg cccaggtggt gacgctgccg
      601 gacccgggat ctctcgccct cggctcgatg cccgtgagcg aagcgattcg gaaccgcaag
      661 agccgcagga agtactccgc cgagttcctc tctcgggagg agctgtcgtt tctcctctgg
      721 gcaacccagg gggttcgaag gcgctcgcca atcaacaccc tgcgcacggt tccctcgggc
      781 ggggctcgcc acgcgctgga gacctatctc tatctgacac gggtcgctgg aatcggggag
      841 gggctgtatc gataccttcc cctggatcac gcactactgg ttctccccgg gcccgagggt
      901 ctggccgggc ggctggacga agcgctcttc gaacaatcct gggacgccgc ggcggtgttc
      961 gtctgggcgg ccgtccccta ccggatggag tggcgctact cctgggtttc ccacaagatc
     1021 atcgccctgg acgccggcca tgtctgccag aacctctacc ttgcgtgcga ggctttgggg
     1081 tgtggaacgt gcgccatcgg ggcgtacgat caggagaaat tggatgcttt gctcggattg
     1141 gacgcgaccg aggagttttc cgtgtatgcg gcgcccgttg gcaggcagat tgaccgtgcg
     1201 gcgacgccat gatttcggcc caatcgagat ttttgcttga cggcgggccg acgttcgtat
     1261 atgatgcggg ttgtattccg gtcgctcttg ggatggtttc gttgaaacag gactgacgtg
     1321 cgcaggaata ccgcgttctg aactagctgg cgcgcaaggg acggactggc acgcgcggga
     1381 ttgcatcaaa aactgtgaat gcagcccaag ggtgaatcag gaagacgttc gggtgacggg
     1441 atcactgtct cgaaggtgta cttctctcaa tcctaccgat caggttcccc catttgccag
     1501 gacagcgttc aatctcgcag ctttgcccgt gatggagcgg gcaggggcgc ggtgagcctg
     1561 gaatcaggcc cacacactct aggatgagcg cgggaacacc gcgcaaaagg gggaggcgac
     1621 atggcaaaga aaatctatgt cggcaacatg aactacaaca cgagcgaaga cacgctgcgg
     1681 cagctcttcg cgcagtacgg cgaggtgatg tcggtaaacg tcatcaccga ccgctacacg
     1741 ggccgggcca agggcttcgg tttcgtggag atggcgtctg aggacgccgc gcgggcagcc
     1801 atgactgcac tgaacggcaa ggaggtcgac ggccgtcagt tgaaggtgaa cgaggccaac
     1861 gagaagcctc gggatgccag ccgcggcggc tacggaggcg gcaggggcta ctaggaacct
     1921 gaccgcaggg acgggcggca cggatccctt atggatccgc attcgagaga cccccggggt
     1981 ctccgaggga cgccgcccgt tcctcccttt ctcccttccc gtcagctccc cttcagaccc
     2041 gccttcttct cgatcttggc ccacgtatcc ttcagcgaaa tagtgcggtt gaaaacgagc
     2101 ctctccgagg agtccggatc cacgcagaaa tagccgagtc tctcgaactg gaagcgctcc
     2161 ccgattcgcg cggcggacag ggaccgctcc accttgcagt ccgatatcac ctccagggat
     2221 cccgggttga gcgtatcctt ccagtccaca cctggtggta cctcctgggg atactccgcg
     2281 gagaacaggt ggtcgtagag tcgcgcctcc gcgctcaggg catgctcggc cgagacccag
     2341 tggatggtcg ccttcaccga gcggttgtcg ggagcgtttc ctccccgggt agccggatca
     2401 taggtgcagc ggatctcttc gacggcaccc gtggtcggat ccttccgaag acccgtgcat
     2461 cggatgatgt aggcatagcg gagcctcacc tgttgaccgg gagagagccg gtagtacttg
     2521 ggcggcggca cctcgcggaa gtcatcggac tcgatgtaga gggtcccggt gaacgcaacc
     2581 tttcgcgtcc cggccgcggg gtcttccggg ttgttcaccg cctcgatctc ctccacctgg
     2641 cccgacggcc agttctcgat caccagcttc acgggacgaa gcaccgccat gacccgcggg
     2701 cttcgacggt tgagatcctc ccgcaagcag tgctccagaa gtgccacctc cacggtgctg
     2761 gaggtcttcg cggtgccgac ccgggatacg aagtcccgga tggcttcggg tgtgtagccg
     2821 cgccggcgga gacccgcgat ggtcggcatc cgaggatcgt cccatcctcg gacccgccca
     2881 tccttcacga gctccaggag ccaccgcttg ctgaggaccg tgtgtgtcac gttcaaccgg
     2941 gcgaactcga tctggcggct gtggaagatc ccgaggttct cgaggaacca gtcgtagagg
     3001 ggccgatgga tctcgtactc caggctgcac agggaatggg taaccccttc gatggaatcc
     3061 gagagggggt gctgatagtc gtacatcggg tagatgcacc aagcgtcccc ggtgcggtga
     3121 tgctgggcgt ggcggatgcg gtacatcacg gggtcccgca tgtgcagatt ggggtgcgcc
     3181 atgtcgatgc gcgcccgcaa cgttcgggct ccgtcgggga actccccggc tcgcatcctg
     3241 cggaacaggt ccaggctctc ttcgggggac cggtttcgat atgggctgtc ccgtcctgga
     3301 ggagtgacgg tcaccggccc cttgtccgtg acgtcggtgc cgcggtattc gcggatctcc
     3361 tgttcgctca gatcgcagac gtacgccttt ccgtcccgga tcagcttctc cgcccactgg
     3421 tagagttgct cgaagtagtc cgaggcgaaa aactcccgat cctcccaatc gaagcccagc
     3481 cagcgcgtgt cctccttgat gccgtcgacg tactcctgct cttccttggt agggttggtg
     3541 tcgtcgaagc gaaggttgca ctttcctccg aagctctcgg cgatcccgaa gtcgatgcag
     3601 atcgccttgg cgtgtccgat atgcaggtag ccgttcggtt cggggggcca acgggttctc
     3661 acccgatcga aacgaccaga tcgcaggtcc tcctgcacgg catcccggat gaaatcgctc
     3721 ttgggggcat cgcggtccag gccggatgcg gatacggcgg aatcggtcac agcattctcc
     3781 ttcccaacgc ttcaggggcg cggtacgacc atagtggggg agacgggggg gcaggtgtca
     3841 aggaggaacg agagagaaga cggagccgat ttcgccagcc gtcagtccag gagcctcttc
     3901 tgcccttgaa tccgctgaag gcgattcagc ttctgggagc actccaggca tccaaggtag
     3961 cggccggtgg aatcccgcag cgcgaagtac tggatcagca ccttctccgg ctgctcctgg
     4021 tcggggagcc tcatgtcgag ccagaactcg gcggagtctc gcgatccctg tctcatctcg
     4081 gagatgatct gctcgaccat cttcaggctc ttgctgggat ggcattggcg cacgtcgcgg
     4141 cccagaacgg tctccacacg gcggaagagc cgggtctcat gccggttcca ggccatgact
     4201 ttgtcgttac cgtcgacgat cgagaactct atcggcaggg catccaggag cgccgagagg
     4261 accggctccg gaatctgtcc cattaccatg gaatgatcct ccacttgggg cggcgtagag
     4321 ccccgccctt ggggcggcgt agagccccgc ccttcgggcg gcgtagagcc ccacccttgg
     4381 ttggtgtagc gttgtgagcc ggcgcagttt accagaaggt gtccacgcga tcaaccgcgc
     4441 gcggctcggg tggctcgggt cactcgggtg ccaacgcgca tggccctgga aacggacgcg
     4501 cgcctcggga agggcatgcg cctggggtca ccttcaggct gccagccctt gtgggtcggc
     4561 gaaggccgga ttccatgggt gtccctccag atcgccactg cgagccttgc gggcgggaac
     4621 ctttcggtac aagagaagga acacgccgta caccacgagg gtttggacgg cccccaacac
     4681 ggcgaccacc aggggatcgc gaagcgcgac cacggtggct acgctcaccg cggagcctcc
     4741 aagggatccg atggaatccg ccatggcgca ccgacggtca aaggcggccc tcccacgtcg
     4801 atagcgaact tccttctcct tctgaagcag atatacgaca gacgtgccga cggtccccag
     4861 gatgaacatc gaggccaggt agtaggccat cacgttgacc gccgccacca ggacggaagc
     4921 cgcggacgcg gcaagctcga tggccatgat cacgggcagg acagcgtaca gcctccgaaa
     4981 gactctcggg ttcgccatcg ccgcgccgat cagaacgggg aggccggaag ccaggaagga
     5041 tcccagggca atgattctcc cgtcaaccct ggagaggatt acgctcacgt acagaggatt
     5101 gaggaacccg taaaagaagc tggtcagaag cccgctgagg acgaatacat ccaggtactt
     5161 cttgggactg cgcatgttcc acctcccgtc gcgggagatg aacggcatcc cccgcgtgct
     5221 aggtcaacct acccccaggg tcatcggtat gttccataca cgctcctgtg cgtctctggt
     5281 ccgccctcag gcggcccctc tgagtgtagc accggagtgg gtgctccggc aactcgatgg
     5341 cctccctccc ctcctggcgg cgctgccggg tacagcgggt ttcatcagga tctgccgcga
     5401 cgcaggatct gttggacatc gagccacccc cctgagaccg cataatccat cgctgtcttg
     5461 ccctgtgggc tgcgccgcga caggtcagcg cccgcctgca gcaaccgggt caccacgacg
     5521 gccagtccgt tggcggccgc gatcatgagc ggagtgacgc ccgcgacctg gtaatccggc
     5581 tcggcgccat ggtccaggag aaggatcgcc accggcggcc tcctgccggc gcaggcgctg
     5641 tgcagcggat gattggcctg ggcgttgtgg gccaccgcgg aagggtcggc gcctgcttcc
     5701 aggagtctcc ccgcggctcg ctcgctccca aaaaacgcgg cgagatggag cggagtccac
     5761 ccgtcggggg aatacgcggt ggccaggtcg cgccgatccg ccaggattct ctccaggcgc
     5821 ccgtcgagcc ccagggcgga ggccacgaac acatccaccg gggcgcctgc cgtcagaagg
     5881 aggtcagcga tatcccgctg cccgcgatag agggcgtaca ataccgggct caccccatca
     5941 ggcgcccgaa tgctggccgt tgccggctcg gactccagca ggtccgcgac ggcatcccgc
     6001 cgaccggcat cgatgaggtg aagtagatcc tgttccatct caagctcaat atacgaatgg
     6061 gggctgcctc cgtcagggta tcccgcctgc aggcgcctct cagcctgtcc cgccatcctt
     6121 gcccgtggcc cggaggcgcg agtacaatgg ttgggaaatg acgccaaaga aaaactacct
     6181 gatcgacatg gacggcgtgc tggtgcgcgg ttccactccc gtccccgggg ccgatgggtt
     6241 cctgggccga ctcaaggagc gggccctcga gtacctggtc ctgacgaaca atccccagta
     6301 cacccagaac gatctctcct accggctcgg cagcgtcggt cttccaatcg agccgcgacg
     6361 aatcttcacc tccgcaatgg caacggcgag cttcctcaag tctcaagaga gcaaccgaag
     6421 ggtctttgtg atcggagaaa gcgggctcac gggtcccctg cacgaggccg gcttcatcat
     6481 cacggacatc gatccagcct tcgtcgtcct cggcgagacc ccatccctta gctatcagca
     6541 gatcaccgcg gccatccgcc tcatctcgag cggcgtccgg ttcatcgcca ccaacccgga
     6601 tgccaacgga cccggagagg gaggcacggt ccccgcctgc ggagccctgg cagccttgat
     6661 cgagaaggcg tccggagtga gcccgttctt cgttggcaag ccgaacccgt tgatgatgcg
     6721 gaccgcgctg aactatctcg gggtgcactc cgagcacacg gtgatgatcg gggatcgcat
     6781 ggacacggac atcgtggccg gagtcgaaag cgggatggaa accatcctgg tcctgagcgg
     6841 agtcaccgcg cgctcggagg ttgcgcgttt cccgtatcgt cccgcaagca tccaggactc
     6901 cgtggcggat atcgaggtct agcgggaccc ctgcggctcg gccgcccagg gcgcgggcta
     6961 tgccttgccg gccttggcgg actgcaaggt gttgtacaga agcatcgtga tggtcatggg
     7021 accgacgccg ccggggaccg gcgtgatggc agacgccttg cgcgagacct cgtcgaactt
     7081 cacgtctccc accatccgct gacctttggg atgcgtcggg tcctcgatgc ggttgatccc
     7141 gacgtcgatg accaccgccc cttccttcac catgtccgcg gtcacgaact ccgggcgccc
     7201 gatggccgca atgaggatgt ctgccagccg cgtgacggac ggcagatccc gggtccgtga
     7261 atggcagacg gtgaccgtgg cgtccgcatc cgcctccttc tgcatcagca tattggcgat
     7321 cggcttgcca acgatgttgc ttcggccaac caccaccacg tggctccccg ccaccgtgac
     7381 cccggagtag gagaggagct tctggatgcc caggggcgtg cacggtcgga agccaggcgt
     7441 accggtcatg atgcgcccca ggctcacggg gtggaacccg tctacgtcct tgtcggggac
     7501 gatcgccgcg atgatcttct gctcgtcgat gtgatccggc aggggaagct gcaccaggat
     7561 gccatggatc ttcgggtcgt ggttgagccg atccacgacc gcaaggagct ccgactccgt
     7621 agtcgtcgcc gccaggttgt gctgctcgga gtagatgccg atctcgtgcg cggccttgtt
     7681 cttccccttg acgtagctct tcgatgccgg gttgtctccc accaggacta ccgccagccc
     7741 cggcgtgatc cctcgctctt tcttgagggc ttcgacaccg gactggacct cggcgcggat
     7801 acggcccgag atctccgtac catcgatgat ccgtgctgcc atatcaggtc tcctcctcag
     7861 gccatgcctt cgagcttgcc ctcgcgctcg gcggtgatgt agttcatgca gaactggtcc
     7921 tttcctgcca gggcctccgc ggcggccacg gtggccatca tacgcgcgtc caggggatcg
     7981 atgatcgcgg agtccatgcc cttggcgatg gccatggcca tgaagacctg gttcacgagc
     8041 tttcgcttgg gcagcccgta ggagacgttg gagaggccgc aggtcttgtg gacctgcggg
     8101 tgcttcgcgg tgatccgctc gacgatggag agagcgccca tgccgtactc cccgttggtg
     8161 ctcaccggcc ggatgagcgg atccacgaag atgtggtccg gaggcacccc gtcgtcttcc
     8221 agccgtcgga tcaggccatc cgcaacctgc cagaccttgt cgaagtcatc gctcattccg
     8281 ctgtcatcca ggctcagcgc gaccacccgg gcgccatact tctttaccag ggggatgagg
     8341 gtcttgtagc ggtcggactc ggccgagatg gaattgagga agggttcctt cccggccatg
     8401 gcgagcccgg cttccacggc agcggctcgt ggcgagtcga ccgacagggg ggcgtccgtg
     8461 acttcccgga tcgtcgtgat gagccagcgc atcttctcca cctcgtcgtt ggggaaggcg
     8521 cccgcgttca cgtcgatcca gttcgctccg gccgctgcct gccgcttggc caggtcctga
     8581 aggtaggcgc cgtccgcctt ctcgaccgct gcgcggatct gcttgcgggt ggagttgatg
     8641 agctcgccga tgatgagcat gggaatcacc tcttatgcgg ggaatcgagg catcatagtg
     8701 gcgcctgccc ctgagagtca aggagagggc ggttctctcg cggctctcgg gacgcctcgg
     8761 ttgaccgcgg ccggttcccg ccctacactc gggggtcatg gagaacttca ccttccagaa
     8821 cccaacccga atcctcttcg gccgcggcgc ggagctccag gcgggggcgg aggcggcacg
     8881 gcatggaagc cgcgtgctcc tccactacgg cgcgggaagc atcaaggcgt ccgggctcta
     8941 cgaccgggtc tgtgcctccc tgcgggacgc caaggcgccg ttcgtggagt tgggcggcgt
     9001 gaaacccaac ccccgcctga gcctggtgcg ggaagggatc cggctttgcc gggagcagaa
     9061 gctcgacttc atcctggcgg taggcggcgg aagcgtgatc gactccgcga aggcgatcgc
     9121 ggtcggcgtc ccttacaccg gcgacgtgtg ggacttctac acgggcaagg ccaaggtgga
     9181 gcggtcgcta cccgtgggtg tcgtgctgac catccccgcc gcgggaagcg agtccagcaa
     9241 cggttcggtg gtaaccaacg atgacggagc gctcaaacgg gacacggggg cggaatgtat
     9301 gcgtcccgtg ttcgcggccc tcaacccgga gcttaccttc accctgccgc cctaccagac
     9361 tgcgtgcgga gccgcggaca tcatggcgca cgtgatggag cgctacttca cccccgccaa
     9421 gggcgtcgac tttacggacc ggctgtgcga ggccaccctt cgcaccatca tcgacaccgt
     9481 gccccgcgct ctggcggagc cccgggacta tgacgctcgg gccaacatcc tctgggccag
     9541 caccatcgcc cacaacgacc ttctggggac cggtcgggtc ggagactggg cttcccacat
     9601 gatcgagcac gagctttccg ggctctacga cgtggcccac ggagccggtc ttgccgtggt
     9661 gttccctgcc tggatgcgcc acgtctaccg caacgatacc gggcggttcg cccggttcgc
     9721 gtgccaggta tggggcgtgg agccgtgctt cgaggacccc gagcgcacgg ccctggaagg
     9781 catccggcga accgcggcct tcttccatca gatcgggctt cccacaacgc tgaaggagct
     9841 cggtgttcca caggcccggc tcgccgagat ggcgcacaag gccgtctggc ggggccccct
     9901 cggcaacttc cgcaagctcg atgaacgcga cgtgctggag gtctatcggc tggccgggta
     9961 ggcaggcgcg cgggagggcg ctactctttg ccgtcgaagg agtagcatac ctcctggtac
    10021 ttcgagcgca tctccttgac ctcgtctccg acgaacttct tgtccacgag gcacttcttg
    10081 aagagctccg cgatgtgctc catctcgggc tccctcatcc cgacacgggt catctcctgg
    10141 acccccatgc gaatccccgc cggggcggtg gccttgtcca gcgtctcgaa ggggagaagg
    10201 ttcatgttga cgatcaggtg gttgtccttg aggtcccggg cagccgtgtc gccgccgccg
    10261 aagtctccca tgtccaccgc cacctggtgc gtctccgtga agtcgaactg ctcgccctgg
    10321 acccggaacc ccagctggtg gagctttcgg gccagcgcct tcgcgttggc caccgtttgc
    10381 cgcgcgtact ctacgccgaa ctccagcatc tcgtaggtcg ccatggtcat ggtgaccaga
    10441 gtctccaggt ggtggttcga tgaggagccg ggaaaggccc ccttgtcgat ccgccgccat
    10501 tcctcgtcgt ccacgttggc caggataagc ccacgctggg aaccgaagaa cgtcttgtga
    10561 gtgctggcgg tgaccagcgg cgccccttca cccagaggat cctggaactg gtgcccggcg
    10621 atgagaccca gcacatgggc cccgtcgtag atgagggtga tcccgcggcg tttgcacacc
    10681 ggcgcgatct ccttcacggg ctcggggaag aggaacaggc tcttgcccat gatcaggacc
    10741 ttgggctcca gggctttcat caggtccagg gtcttttcga cgtcgatgtg gtacccgtcc
    10801 gcggtgagcg ggaagctcac gatgttcttg gtgtatttgc cgacggaccc ggcgcgatgg
    10861 tggctgatgt gcccgccccc tggcgtggag ttgaccatgg cgacatcccc aggccggatg
    10921 tagcggctga agacggcgtc gttggcgatg gtgccgctga tggggcgcac gtcgctgtgc
    10981 ttgcagcgga agagggcctt catgtgctgc ttcagcaggc tctcgatctc gtcgatgatc
    11041 tccgtgccct ggtagtagcg ctcccccgga tgaccttccg cgtagcggtg ggagaagtcc
    11101 gaccccatga cgccgcgcac ccgccggctg gtcacgttct cactggcgat caggttgatg
    11161 cagtggcccc gccagtcgtt ctgctcccgg accagctcct cgatgtgggc cacgtttctc
    11221 tcgtacatga atggtttcct ctcggcgcgt tacagcccgg ccaggaactc gaagacctcg
    11281 tcctcgacgc cggtgatgct ctcatgcgcg aaatccggat agatgatcgt gcgcttcttg
    11341 cacgtcatgc ggttgtaggc ggcgaactga gtcgacggcg ggcagacctc gtccatcaga
    11401 cccgtcgcca tgaccacctc cgccttgatc ctgggggcca ggtactgcgc gtcgatgtac
    11461 cccaaccggg tgaagatctc ctcttcacgc cggtgctggg gatcgaacgc tcggaagtac
    11521 caggccagct cctcgtaggc gcgtttggcc tggtccatct cccagacgcg tcggtagtcg
    11581 cacaggaacg ggaacacggg catggctttg gcgatgcgcg gctcgagcga ggcgcaggcc
    11641 agcgtcagcc cgccgccctg cgacccgccc atcgccgcca cgcgcgt
//
LOCUS       DASRZS010000225         4819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5516092, whole genome shotgun sequence.
ACCESSION   DASRZS010000225 DASRZS010000000
VERSION     DASRZS010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5516092"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFH83_05690"
     CDS             <1..807
                     /locus_tag="VFH83_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6485889.1"
                     /translation="ADGTFFTDEDNRARRNVVVLGKDVVTNLFGDQEPVGRSIKIAGA
                     NFRVVGVLEQKGAGGGFFSQDDTAIIPILTMYTRFRHQKDVRSIGMTVQDKDQIDAVK
                     SEITDLLRTRHKIQDGADSDFAIVSQQDVLQTVQGVSQVMTLLLGSIAGISLLVGGIG
                     IMNIMLVSVTERTREIGIRKALGARRRDILAQFLVEAIILSGLGGLLGWALGALGANL
                     ISKFGNMAVAITVGTVGLAIGFSIAVGLFFGIYPARKASRMDPIQALHFE"
     gene            820..1635
                     /locus_tag="VFH83_05695"
     CDS             820..1635
                     /locus_tag="VFH83_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485890.1"
                     /translation="MIVKRVASGLLAAALLLVGHALFAQAPAPGQTPIQGFTVQGLIT
                     QLSLYLPEDVTRGMFAGFGGRGLGAAAGGAGAGGGAGAAAGGAGGAAGAGGGAQGGGV
                     GSGAGGAGAGGAGGPGAGAQRFTFNFSRDPTLFLTKDQITSLIPILTALRENPMPTPS
                     KARQVQASVDALLTVAQKAEYGQFQKAVEQARQSFQQNRGGGQSFPDFRTMTPEQRQQ
                     FLDSLPADQRQRLQAFLSRSGQQGQLTPLELRQRQLDAFVKALQERLKQIQGA"
     gene            1658..2350
                     /locus_tag="VFH83_05700"
     CDS             1658..2350
                     /locus_tag="VFH83_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6485891.1"
                     /translation="MQASILIIEDVREMADLIRLYLVKEGMACSIAESAEAGLSAFAA
                     DRFDLVILDINLPGIDGFEFLQRIRKTSSVPVMIVSARDTDEDMILGLGIGADEFVTK
                     PFSPKVLVARVRAMLRRASDLKSSPNAVKFGDFLLDIDGYLLKRGGEKIPLSTKEFEV
                     LTFLVTHPGRAMTPDTIYSEVWRNQYGDITAVAVYIQRLRKKIETDPSSPMFIETVHG
                     MGYRFNAEAIHA"
     gene            2347..3732
                     /locus_tag="VFH83_05705"
     CDS             2347..3732
                     /locus_tag="VFH83_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6485892.1"
                     /translation="MKLRTQFVLLVGGIIAVPFLVAAFVLLVQYRMDRDRQPLPNYGM
                     ITQWIRRQVPWAVNHHEYAALRASRPPGLDIMLVDGTNTISFSTIPEIGAGISATSGA
                     LLEYIRRNSDSFHFQIDATRNPEASGNALMILKVPRIRPEEQTFRNQFLAIVTYSAIA
                     LLVFSSLMSFLILRSLNRSILKLEGATRRIAEGDLDFELSARGNDQISSLTRSFDSMR
                     RALKEEYARRSRFIMGVSHDLRTPLTLIQGYVEAINDGLAPEPDSQKRYLSIILDKTR
                     ALEGMITDLIEFVRMETGQWRMTHRDVTIQTFLTENARRFAEDAFILKRDFGWSINIP
                     PEAAVRMDAGLFSRALENLVGNAIRYTSPEGKINLSARLDGEHVLMSISDTGIGIPPE
                     DLSRIFDPFYRGTNSRREQGFGLGLTTVKSIIESHGWAIGVSSQLGQGTTFTIRMPLA
                     TSTPPAAGAAA"
     gene            3729..4145
                     /gene="arfB"
                     /locus_tag="VFH83_05710"
     CDS             3729..4145
                     /gene="arfB"
                     /locus_tag="VFH83_05710"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HET6485893.1"
                     /translation="MTRTELARIVAARAELSYARSGGPGGQNVNKVATKAIARLPLDS
                     LDFLSDAERRLVASRLANRITGGGWITVAAQDTREQSRNRELAVQRMAELISRALVRP
                     KARRPTHPGMAAREARLRSKRKHALRKKDRSPTPLD"
     gene            4182..>4819
                     /locus_tag="VFH83_05715"
     CDS             4182..>4819
                     /locus_tag="VFH83_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015232610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET6485894.1"
                     /translation="MPNEASQVETVAYDIVVIGSGLAGMRAALQAARAADGRLKIALV
                     SKQHAMRSHSVSAEGGISGVLFPGEADDSTDLHAADTVKGGDFLGDQPAIETLVAEAP
                     KEIRFFDHLGTPWNRSVEGKITQRAFGGMTVPRTVFAADKTGFFMLHALYDTLLGFPG
                     IDILHEHYATDLLLSGSTLKGFLVLDLATGGLKFLAAKAGIIATGGYARMYN"
BASE COUNT          828 a         1614 c         1609 g          768 t
ORIGIN      
        1 gccgacggga cgtttttcac ggatgaggac aatcgcgccc gacgcaacgt cgtggtcctg
       61 ggcaaggacg tggtcacgaa cctcttcgga gaccaggagc cggtcggcag gagcatcaag
      121 atcgcgggcg ccaacttccg cgtggtcggc gtgctggagc agaagggggc gggaggcggg
      181 ttcttcagcc aggacgacac cgccatcatc cccatcctga cgatgtacac gcggttccga
      241 caccagaagg acgtgcggag catcgggatg accgtgcagg acaaggacca gatcgacgcc
      301 gtgaaatcgg agatcaccga cctcctgcga acccgccaca agatccagga cggggcggac
      361 agcgacttcg ccatcgtaag ccagcaggac gtgctgcaga ccgtgcaggg cgtgagccag
      421 gtcatgacgc tcctcctggg aagcatcgcc ggtatcagcc tcctggtcgg tgggatcggg
      481 atcatgaaca tcatgctggt gtccgtgacc gagcgcaccc gggagatcgg gatccgaaag
      541 gcgttgggcg cccggcgtcg ggacatcctt gcccagttcc tggtggaggc catcatcctc
      601 tccggcctgg gcgggcttct gggctgggcg ctcggagcgc tgggcgcgaa cctcatcagc
      661 aagttcggca acatggccgt ggccatcacc gtgggcacgg tgggtctggc catcgggttt
      721 tcgatcgccg tggggctgtt cttcgggatc tacccggcgc gcaaggcgtc ccggatggac
      781 cccattcagg ccctgcactt cgagtaggag agggagggaa tgatcgtgaa acgcgttgct
      841 tcaggccttc tggccgccgc gctccttctg gtcgggcacg cgctctttgc ccaggcgccc
      901 gctccgggcc agacgcccat ccagggcttc accgtgcagg ggctcatcac ccagctctcc
      961 ctctatctgc ccgaggacgt gacccggggg atgtttgcgg gtttcggcgg acgaggcctg
     1021 ggcgccgccg ccggcggcgc gggcgcggga gggggagcgg gcgcggccgc gggaggagcc
     1081 ggcggcgcgg ccggcgcagg tggcggagcg cagggaggcg gtgtgggctc gggggcaggt
     1141 ggcgcgggag ccgggggggc cggcgggccg ggcgccggag cgcagcgctt caccttcaac
     1201 ttctctcggg acccgaccct cttcctcacg aaggaccaga tcacgagcct catccccatc
     1261 ctgaccgcgc tccgggagaa cccgatgccc acgccgtcca aggccaggca ggtccaggca
     1321 agcgtggatg ccctgctgac cgtcgcgcag aaggccgagt acgggcagtt ccagaaggcg
     1381 gtcgagcagg cgcgtcagag cttccagcag aaccggggcg gcggtcagag cttcccggat
     1441 ttccgaacca tgacccccga gcagcgccag cagttcctgg acagcctccc ggcggaccag
     1501 cgccagaggc tccaggcgtt cctgagccgc tcggggcagc aggggcagct taccccgctg
     1561 gagctccgtc agcggcagct ggacgcgttc gtcaaggcgc tccaggagcg gctgaagcag
     1621 atccagggag cctgaggcag gtccagggag gaatggagtg caggcgtcga ttctgatcat
     1681 tgaggacgtg cgggagatgg cggacctgat ccgtctctat ctcgtcaagg agggcatggc
     1741 gtgctctatt gccgagtccg cggaggccgg cttgtccgcc ttcgcggcgg accggttcga
     1801 cctggtcatc ctggatatca acctgccggg gatcgatggg ttcgagttcc tccagcgcat
     1861 ccgcaagacg agcagcgttc cggtgatgat cgtctccgcg cgggacaccg atgaggacat
     1921 gatcctgggg ctggggatcg gggcggacga gttcgtcacc aagccgttct ctcccaaggt
     1981 gctggtggcg cgggtgcgcg ccatgctgcg tcgagcctcg gacctcaagt cgagccccaa
     2041 cgcggtgaag ttcggggatt tcctcctgga catcgacggt tacctgctca agcgaggcgg
     2101 tgagaagatc ccgctctcca ccaaggagtt cgaggtgctt acgttcctgg tcacccatcc
     2161 gggtcgggcc atgactccgg atacgatcta ctccgaggtc tggcgcaacc agtacggtga
     2221 catcacggcg gtggcggtct acatccagcg gctgcgcaag aagatcgaga cggacccgtc
     2281 ctcgcccatg ttcattgaaa cggtccacgg catggggtac cgtttcaacg ccgaggccat
     2341 tcacgcatga agctgcgaac gcaattcgtc ctgctggtcg gcgggatcat cgccgtcccg
     2401 ttcctggtgg ccgcattcgt cctcctggtc cagtaccgca tggaccggga ccgccagccc
     2461 ctgcccaact acgggatgat cacccagtgg atccgtcgcc aggtgccctg ggcggtcaac
     2521 caccacgagt acgccgcgct gcgggcgagc cgaccgcccg gcctggacat catgctggtg
     2581 gacggcacca acaccatctc cttctccacc atcccggaga tcggcgcggg gatctccgcc
     2641 accagcggcg ctctcctgga atacatccgc aggaacagcg acagcttcca cttccagatc
     2701 gacgccacgc gcaacccgga ggcgagcggc aacgccctca tgatcctcaa ggtgccccgc
     2761 atccgtcccg aggagcagac cttccgcaac cagttccttg ccatcgtcac ctacagcgcc
     2821 atcgcgctcc tggtgttctc ctccctcatg agcttcctga tcctgcggtc cctgaaccga
     2881 tccatcctca agctggaagg cgcgacgcgg cgcatcgccg agggggatct ggacttcgag
     2941 ctctccgcgc gcggcaacga ccagatctcc tccctcacgc gctccttcga cagcatgaga
     3001 cgcgcgctca aggaggaata cgcccgcagg tcgcgcttca tcatgggggt gtcccatgac
     3061 ctgaggacgc cgctcacctt gatccaggga tacgtggagg cgatcaacga cggcctggcc
     3121 cccgagccgg attcccagaa gcgctacctg tccatcatcc tggacaagac gcgggccctg
     3181 gaggggatga tcaccgacct catcgagttc gtgcgcatgg agacggggca gtggcgcatg
     3241 acccatcgcg acgtcaccat ccagaccttc ctcacggaga acgcccgacg cttcgcggag
     3301 gacgcgttca tcctcaagcg ggacttcggg tggtccatca acatcccgcc ggaggccgcg
     3361 gtccgcatgg acgccggcct gttctcccgc gccctggaga acctggtggg caacgccatc
     3421 cgctacacct ctcccgaggg caagatcaac ctcagcgcgc ggctggatgg cgagcacgtc
     3481 ctcatgtcca tctcggacac gggcattggc atcccgccgg aggacctgtc gcgcatcttc
     3541 gatcccttct accgcgggac caactcccgc cgggaacagg ggtttgggct cggtctcacc
     3601 acggtgaagt ccatcatcga gagtcacggg tgggccatcg gcgtgtcttc ccaactcggc
     3661 cagggcacca cgttcaccat ccgcatgccg ctggccacca gcacgccgcc ggcggcggga
     3721 gcggcggcat gacgcggacg gagctggcgc ggatcgtcgc cgcgcgggcg gagctcagct
     3781 acgcccgctc cggcggtccc ggcggacaga acgtgaacaa ggtggccacc aaggccatcg
     3841 cccggcttcc gctcgattcc ctggacttcc tgagcgacgc cgagcgaagg ctcgtggcct
     3901 cgcggctcgc caatcggatc accggcggcg gctggatcac cgtggccgcc caggacacgc
     3961 gggagcagtc gcgaaaccgc gagctcgcgg tccagaggat ggcggagctg atctcccgcg
     4021 ccctggtgcg gcccaaggcg cgcaggccga cccatccggg gatggccgcg cgggaagcgc
     4081 gcctgcggtc caagagaaaa cacgcgctgc gcaagaagga ccggagcccc acgcctctcg
     4141 attgattgat ccaagccccg gatcgtgagt atgcttgccc tatgccaaat gaggcgtccc
     4201 aggtcgaaac cgtagcgtac gacatcgtgg tcatcggcag cgggctggcc ggcatgcgcg
     4261 ccgccctcca ggcggccagg gccgcggacg gcaggttgaa gatcgccctc gtctccaaac
     4321 agcacgccat gcgaagccac tcggtctccg cggagggcgg gatctcgggc gtcctttttc
     4381 ccggagaggc ggatgactcc acggacctgc acgcggcgga cacggtgaag ggcggggact
     4441 tcctggggga ccagccggcc atcgagacgc tggtggcgga ggctccgaag gagatccggt
     4501 tcttcgacca cctgggcacg ccctggaacc ggtcggtgga ggggaagatc acccaacggg
     4561 cgttcggcgg tatgacggtc ccgcgcaccg tgttcgccgc ggacaagacc ggcttcttca
     4621 tgctgcacgc cctgtatgac acgctcctgg gctttccggg gatcgacatc ctccacgagc
     4681 actacgccac ggatctgctg ctcagcggct ccaccctcaa gggcttcctg gtgctggacc
     4741 tggccaccgg gggtctgaag ttccttgccg ccaaggcggg catcattgcc acgggcggct
     4801 atgcccggat gtacaactt
//
LOCUS       DASRZS010000226         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2820574, whole genome shotgun sequence.
ACCESSION   DASRZS010000226 DASRZS010000000
VERSION     DASRZS010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2820574"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFH83_05720"
     CDS             complement(<1..328)
                     /locus_tag="VFH83_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter"
                     /protein_id="HET6485895.1"
                     /translation="MEQPFDPGITQKFDGAIRRIINPDGSFNVRRRGRRLSDLHLYQL
                     LVRLSWPAFLLVILLTFLGITVAFTGLYFAVGLGGLQGIPDGTPLQRFLQVFFFSIQT
                     LTTVGYG"
     gene            419..1657
                     /locus_tag="VFH83_05725"
     CDS             419..1657
                     /locus_tag="VFH83_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6485896.1"
                     /translation="MESRSSPAAEPTTRAAQTTVVPILIAISLSHMLNDTLQAVIPAI
                     YPLVKDAFHLNFAQIGLITFTFQLTASLLQPAVGALTDRRPQPLSLATGMTFTLTGLV
                     LLSLTASYGLLLVSVALIGLGSSVFHPESSRVAHMAAGQRRGLAQSVFQVGGNFGSSL
                     GPLLAAVVLASRGTSRVIWFTPVALVGIVLLAWIGGWYRGRLAETGGRATQVRRPDGP
                     QLSPGRVTLSVAILLVLIFSKYFYLTGMTSYYTFYLIDKFHLGVQAAQFHLFLFQFAV
                     AAGTVIGGPVGDRIGRKYVIWGSILGVAPFTLVLPYVGLSWTTALAFPIGVILASAFP
                     SILVYAQELLPGNIGLISGLFYGFAFGMGGIGSAVLGAIADHTSIQHVYRLTAFLPLL
                     GLITVFLPNLRRAAPKPAAR"
     gene            1750..>2009
                     /locus_tag="VFH83_05730"
     CDS             1750..>2009
                     /locus_tag="VFH83_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485897.1"
                     /translation="MSSVRFASRGELAGFEWDNQRKDWKRPPVDKQVLKDLSTRSTWN
                     GIARVAYFMLLLAIPLLAALYVSRLNLWLAIPVLYVYYFFYG"
BASE COUNT          313 a          664 c          625 g          407 t
ORIGIN      
        1 ttccatagcc gaccgtggtg agcgtctgga tgctgaagaa gaacacctgg aggaagcgct
       61 gaaggggcgt gccgtccgga atcccctgca gcccgcccag acccacggcg aagtacagcc
      121 ccgtgaacgc cacggtgatt cccagaaagg tgagcaagat tacgaggagg aacgcgggcc
      181 acgaaagacg gaccagtagc tggtagaggt gcagatcgga cagacgacgt cccctgcggc
      241 gcacgttgaa cgagccatcc ggattgatga tcctgcggat ggcgccgtcg aacttctgag
      301 tgatccctgg gtcgaatggc tgctccatcg gaagaggata ccgacacccg gccgaccgga
      361 cgagtgccgg tccagttgtt tcagccaccg gcgggtggtt atcttcacgg taccgatcat
      421 ggaatccaga tcctctcccg cggcagagcc gaccacccgg gcggcgcaga cgactgtggt
      481 ccccatcctt attgcgatca gcctgtcgca catgctgaac gacacgctgc aggccgtcat
      541 cccggccatc tatccgctcg tcaaagacgc gttccacctc aacttcgccc agatcgggct
      601 catcaccttc acctttcagc tcaccgcttc ccttctccag cccgcggtgg gcgcgttgac
      661 ggaccgcagg ccgcagcctc tgtctctggc gacgggcatg acgttcaccc tcacaggcct
      721 ggtgctcctg tccctgacag cgagctacgg actgctcctg gtgtccgtcg ctctgatcgg
      781 gctgggctct tccgtgttcc atccggaatc ctcccgggtg gcgcacatgg cggccggtca
      841 gcggcgaggg ctggcccagt cggtattcca ggtgggcggc aacttcgggt cgtcgttggg
      901 cccgctgctt gctgcggtgg tgcttgcttc tcgggggacc tcccgggtca tctggttcac
      961 accggtggcc ctggttggga tcgtgctcct ggcctggata ggaggctggt atcgcggaag
     1021 gctcgcggaa accggaggac gggctaccca ggtcagaaga ccggacggcc cgcagctatc
     1081 cccgggccgt gtaaccttgt cggtcgcgat cctgcttgtc ctgatcttct cgaagtattt
     1141 ttacctgacc gggatgacga gctactacac cttctacctg atcgacaagt tccacctggg
     1201 ggtgcaggcc gcccagttcc atctgtttct gttccagttc gcggttgcgg ccggcacggt
     1261 gatcgggggg cccgtcggag accggatcgg ccgcaagtac gtgatctggg gctccatcct
     1321 gggcgtagct ccgttcaccc tggtgcttcc ttacgtcggg ctgtcttgga cgacggccct
     1381 cgccttcccc atcggggtca tcctggcttc cgccttcccg tccatccttg tctacgcgca
     1441 ggagcttctg ccggggaata tcgggctgat ctccggcctt ttctacggct tcgcgttcgg
     1501 catgggcggc atcgggtccg ccgtcctcgg cgccatcgcc gatcacacca gcatccagca
     1561 cgtctaccgc ctgacggcgt tcctgccgct tctcggcctg atcacggtgt tcctgccgaa
     1621 cctgcgacgc gcggcgccga agcccgcggc ccggtgaccg gggagggacc gggcccatgc
     1681 gtttgctggc ctccacggtg gcatagtaca ttcgtctcag tgtccgccgg acaccaggag
     1741 ggaaacgcca tgtcgagtgt acggttcgcg tcgcgggggg agctggccgg cttcgagtgg
     1801 gacaaccagc gcaaggactg gaagcgccct cccgtggaca agcaggtcct gaaggatctg
     1861 tccacgcgca gcacgtggaa cgggatcgcc cgcgttgcct acttcatgct ccttctggcg
     1921 atccccctcc tggctgctct ctacgtgagc cggctcaacc tgtggctggc cattccggtg
     1981 ctctacgtct actacttctt ctacggctt
//
LOCUS       DASRZS010000227         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5131245, whole genome shotgun sequence.
ACCESSION   DASRZS010000227 DASRZS010000000
VERSION     DASRZS010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5131245"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..287
                     /locus_tag="VFH83_05735"
     CDS             117..287
                     /locus_tag="VFH83_05735"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HET6485898.1"
                     /translation="MSADSTKVKDVVCGMMVDPSTASARRIYETKKYHFCSLGCAKAF
                     DADPKRYITAGT"
     gene            313..921
                     /locus_tag="VFH83_05740"
     CDS             313..921
                     /locus_tag="VFH83_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6485899.1"
                     /translation="MLRDGTDVHRAKGLVILIHGRGASAEDILSLAPELGLEGFAWWA
                     PQATNSTWYPYRFVEPPERNEPWLSSALALVAGMVQEAGELGMPKERIWIAGFSQGAC
                     LSLEFAARSGTRWGGVAAFSGGLIGDRIYRENYPADLGGCPVFIGSSDPDPHIPVPRV
                     RESAVVLRELKASVVERIYDGMGHTISADELEEARAHVFGGA"
     gene            complement(926..1447)
                     /locus_tag="VFH83_05745"
     CDS             complement(926..1447)
                     /locus_tag="VFH83_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485900.1"
                     /translation="MLASTFRRLVTMDTSDFLESFLRCLEDARVPYCVVGGQAVNAYV
                     EPLVSLDLDVVVAAAEVGRIEEQLRSRFRIESFPHSLNISQAGSDLRVQIQTDPRYAG
                     FPERARTGNVLGRQMPVAAVDDVLRGKVWAASDPTRRGSKRQKDLADIARLLERFPGL
                     RAEVPENLLSKLL"
     gene            complement(1511..2659)
                     /locus_tag="VFH83_05750"
     CDS             complement(1511..2659)
                     /locus_tag="VFH83_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485901.1"
                     /translation="MTSKERVKIVLDHGKPDRVPFFEQGVASNVASDILGRPACTGGG
                     RFRRDGIEAALAGDDAYRDYLVRGATDWGDLVEALDFDVVTLPWFGGGAPCNRIDENT
                     YLFRSDDGTDWSVVRFQPESDTLHTVDSAARQGDLEWLDTRVRALEDAHRARKPITAD
                     SYPELQYAIQRFAAKRAIGGGAGIAIPMEPVWLEAIAARPDLIERYLDIQADVGCEAI
                     AVQAGMGIDLVWGGGDFVNNAGPLYSPEHFRALMLPRVRRLTETTHRFGLKYIFRTDG
                     NVWPVARYLFQESGVDGYGEIDRQAGMDLGQIRARMPHLILWGNVDCGRTLTAGTLEE
                     VRAETRACIDKAGPGGGYILGSSNVIHSQVPTASFTAMIDECRSYGVY"
BASE COUNT          474 a          885 c          874 g          440 t
ORIGIN      
        1 caagcagcac atgaacacct gcgtgaagga cgcgatccag aagaatcgtg ggagtgaaaa
       61 gatcgacgaa atcactatga tcctcgagaa gtatctcgag agatgaggag tcaccgatga
      121 gcgcggattc cacaaaggtc aaagacgtgg tgtgcggcat gatggtggat ccttccaccg
      181 cttccgcacg gaggatctac gagaccaaga agtatcactt ctgctccctg gggtgcgcca
      241 aggcgttcga cgccgacccc aagcgataca ttaccgccgg gacgtagcct gcgtgacgct
      301 catgagcgcc ggatcctccg agacggcacg gacgtccaca gggcaaaagg tctggtgatc
      361 ctgatccacg ggcggggggc atcggcggag gacatcctgt cccttgcccc cgagctcggt
      421 ctggaaggat tcgcctggtg ggcgccccag gccaccaact ccacctggta cccctaccgg
      481 ttcgtggagc ctcccgagcg caacgagccg tggctgtcct ccgcgctggc cctggtggct
      541 ggcatggtcc aggaggcggg cgagctcggg atgccgaagg aacggatctg gatcgccggc
      601 ttctcccagg gcgcgtgtct gagcctggag ttcgcggcgc gaagcggaac gcgatggggg
      661 ggcgtggccg ccttcagcgg cggcctcatc ggggatcgga tctatcggga gaactatccg
      721 gcggaccttg gggggtgccc ggtgttcatc gggtccagtg acccggaccc gcacatcccc
      781 gttccccgcg tgcgggagtc cgcggtggtc ctccgggagc tgaaggcctc ggtcgtcgaa
      841 cggatctacg acgggatggg gcacacgatc agcgccgacg agctggagga agcgcgggcc
      901 cacgtgttcg gtggggcctg aggactcaga gcagcttcga aagcaggttc tcgggaacct
      961 cggcgcggag ccccgggaac ctctccagca gccgcgcgat gtccgcgaga tccttctggc
     1021 gcttgctgcc gcgacgggtc ggatccgagg ccgcccaaac cttaccgcgg agaacgtcgt
     1081 ccaccgccgc caccggcatc tggcggccaa gaacgttccc ggtcctggct ctctctggaa
     1141 aaccggcgta ccgagggtcg gtttgaatct gcacgcgaag atctgaaccg gcctgcgaga
     1201 tgttcaggct gtgggggaat gactcgattc tgaatcgcga tcgaagctgc tcctcgatac
     1261 ggccgacctc cgcggctgca acgacgacgt ccagatccag gctgaccaac ggctcgacat
     1321 aggcgttcac cgcctgccct cctacaacgc aatacggcac gcgagcgtct tccagacacc
     1381 gtagaaaaga ctccaggaag tcgctcgtgt ccatggttac gagccgtcgg aatgtcgaag
     1441 cgagcatcgc atgtccccca gcgctcctcg gataagcata ggagcgacgc gcccgacgat
     1501 caagccgccc tcaatacacc ccgtaggacc tgcactcgtc gatcatggcc gtgaagctgg
     1561 cggtgggaac ctgggagtgg atcacgttcg aggagcccag gatgtaccct ccgcccgggc
     1621 ctgccttgtc gatgcaggcc cgcgtctccg cgcggacctc ctccagggtg cccgcggtca
     1681 gcgtcctgcc gcaatccacg tttccccaga ggatgagatg gggcatccgg gcgcggatct
     1741 gtccaaggtc catgcccgcc tgtcggtcga tttccccgta cccgtccact ccggattcct
     1801 ggaacaaata gcgcgcaacc ggccagacgt tgccatccgt gcggaagatg tacttgagac
     1861 cgaagcggtg cgtggtctcc gtgagacggc ggacgcgcgg gagcatcagg gcgcgaaagt
     1921 gttcgggaga gtacagcggc cccgcgttgt tcacgaagtc cccaccgccc cacaccaggt
     1981 cgatgcccat gcccgcctgg acggcgattg cctcacagcc gacgtccgcc tggatgtcga
     2041 ggtagcgctc gatgaggtcc ggccgggcgg cgatggcttc cagccacacc ggctccatcg
     2101 ggatggcgat gccggccccg ccgccgatgg cgcgcttcgc cgcgaagcgc tggatcgcgt
     2161 actgcagctc ggggtaggaa tccgcggtga tgggcttgcg ggcgcggtga gcgtcctcca
     2221 gcgcgcggac ccgcgtgtcc agccactcca ggtcaccctg gcgcgccgcg ctgtccacgg
     2281 tgtgcagcgt atcgctctcc ggctggaacc ggaccacgga ccagtccgtt ccgtcgtcgg
     2341 atcggaagag gtaggtgttc tcatcgatcc gattgcacgg cgcgccgcct ccgaaccacg
     2401 gcagcgtgac cacgtcgaag tccagcgcct ccaccaggtc tccccagtct gtggcgcccc
     2461 ggaccaggta gtcgcggtac gcgtcatcgc cggccagcgc ggcctcgatg ccgtcccggc
     2521 gaaagcgccc gccccccgtg caagcgggcc gacccaggat gtcgcttgcc acgttggagg
     2581 cgactccctg ctcgaagaag gggacgcggt ccggtttccc atgatccagg acgatcttca
     2641 cccgttcctt cgatgtcatg acgtaacctc cgc
//
LOCUS       DASRZS010000228         2398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_900353, whole genome shotgun sequence.
ACCESSION   DASRZS010000228 DASRZS010000000
VERSION     DASRZS010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900353"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFH83_05755"
     CDS             complement(<1..445)
                     /locus_tag="VFH83_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485902.1"
                     /translation="MREPHGAVTPQPHAAIRGGDRARRTRRRTIRYTLLILFAVVAVL
                     LPFWTVLINSAKILREASSLGIGLPTHWQIGPNYNEVITKGKLFRGLRNNLLIVIPAT
                     AGIVLLGSAAAWAFARSRSRRVSLLYWMCISGILIPPAIVTSVVVL"
     gene            complement(442..1347)
                     /locus_tag="VFH83_05760"
     CDS             complement(442..1347)
                     /locus_tag="VFH83_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485903.1"
                     /translation="MASAPARSAIRARGHLWPFLLPAVLLVGLFYILPNLLNFVLGLT
                     NWSSAHSDIRFNNFRNFVDLYQDKTIWSSIWTTVRFAITVMVVENAVALTLALALRES
                     TPLNLVLRLVFFLPVLISPLAAGFIFSGLLAPSGTYNGIFTALGKLVGLAPIDFGWLG
                     SVTVTLYVVACIHAWKFGGVHMFVYVAGLMAIPEELTEAARVEGAGPWHTFAHVTIPL
                     LGPALTFNITLTLIGALSIFDVVLATTKGGPGRVTEVMNMIVYRQFGTGAFGFAVAIA
                     AVLFFIILLVAVPLVTYLRRREVEL"
     gene            complement(1423..>2398)
                     /locus_tag="VFH83_05765"
     CDS             complement(1423..>2398)
                     /locus_tag="VFH83_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6485904.1"
                     /translation="LIDQSDMPFVKNTVAGLLDRAGNYRGKYYAAIIDYPFIDGVFYN
                     KQVFQRLGLKIPTNYQELLAVCAAIKQKAPNVAPIYTGGGDQWPLQVLAFIMWNDDIK
                     ADARMIDDLNANRIKFTDPRFVAGIAKEKEILDKGYYNKDILTAKFTDEQSHLMDGSA
                     AMVVNGSWIVGSLLDGYGLDKVNQTVGFFGLSARSNIVSWQTVGLGEFYRPKTGDDAH
                     EAAAKDFINWVTGSYYKTYLDNSKQFPVMKGYAVPASGVAQASIDANNALLKSSVAQY
                     QQTLQAAYGPFESFLSAMVAGQQTPLQVAQSLDTEFERSARQQGLPGF"
BASE COUNT          462 a          716 c          827 g          393 t
ORIGIN      
        1 gcaagaccac cacggaggtg acgatggccg gcgggatcag gatcccggag atgcacatcc
       61 agtacagcaa cgagacgcgg cggcttcggc ttcgagcgaa cgcccaggca gcggcggagc
      121 ccaggaggac gatcccggcc gtggccggga tgacgatgag gaggttgttg cgaaggccac
      181 ggaagagctt gcccttggtg atgacctcgt tgtagttggg gccgatctgc cagtgggtgg
      241 gaagcccgat gcccagcgag ctcgcctctc gcaggatctt cgccgagttg atgaggaccg
      301 tccagaacgg caggaggacg gccacgaccg cgaaaaggat gagcagggtg tagcggatgg
      361 tcctgcggcg cgtccgacgg gcccgatcgc cgccccggat cgcggcatgc ggctgcgggg
      421 tcacggcgcc gtgcggctct ctcacagctc cacttcccgc cgtcgcagat aggtgaccaa
      481 cggcaccgcc accagcagga tgatgaagaa gagcacggcg gcaatggcca ccgcgaaccc
      541 gaatgcgccg gtcccgaact gccggtagac gatcatgttc atgacctccg tcacgcggcc
      601 cgggccgccc ttggtggtgg caaggaccac gtcgaagatg gacagcgcgc cgatcagcgt
      661 gagcgtgatg ttgaacgtga gggccggccc caggagcggg atggtcacgt gggcgaaggt
      721 gtgccacggt cccgcgccct ccacccgggc ggcctccgtg agctcctccg ggatggccat
      781 gagccccgcg acgtagacga acatgtgaac gcctccgaac ttccaggcgt ggatgcaagc
      841 caccacgtag agggtgacgg ttacgctgcc cagccagccg aagtcgatgg gggcaaggcc
      901 aaccagcttc ccaagcgccg tgaagatccc gttgtacgtt ccggagggtg ccagcagacc
      961 ggagaagatg aacccggccg ccagagggga gatcaggacg ggcaggaaga agaccagccg
     1021 caggaccagg ttcaggggcg tggactcccg cagcgccagc gcaagggtca gcgcaacggc
     1081 gttctccacc accatgacgg tgatggcgaa ccggacggtg gtccagatgg agctccagat
     1141 ggtcttgtcc tggtagagat ccacgaagtt gcggaagttg ttgaagcgga tgtcggaatg
     1201 ggcggaagac cagttggtga gccccagcac gaagttgagg aggttaggaa ggatgtagaa
     1261 gagaccaacc agcaggacgg cgggcagcag gaagggccag aggtgcccgc gggcgcggat
     1321 ggccgaacgt gccggcgcgg atgccatgat cgtaccccta accgaagggg cggaaggagg
     1381 atgcccttcc gccccatacg gagctgacgc ggatggaatc gactagaagc ccgggagccc
     1441 ttgctgccga gcgctgcgct cgaactcggt gtccagggac tgggccacct gcagcggcgt
     1501 ctgctgcccg gccaccatcg ccgagaggaa gctctcgaac ggaccgtagg cggcctggag
     1561 cgtctgctgg tactgcgcca cggagctctt gagcagggcg ttgttggcat cgatggacgc
     1621 ctgggccacg ccggaggccg ggaccgcgta gcctttcatc acggggaact gcttggagtt
     1681 gtccaggtag gtcttgtagt acgaaccggt cacccaattg atgaagtcct tggctgccgc
     1741 ctcatgcgcg tcatctcccg tcttgggtcg gtagaactca cccaggccga ccgtctgcca
     1801 ggagacgatg ttgctgcgcg cagacagacc aaagaagccc acggtctggt tgaccttgtc
     1861 aagcccgtaa ccgtccagca ggctgccgac gatccagctt ccattcacca ccatggccgc
     1921 ggacccgtcc atcaggtggc tctgctcgtc ggtgaacttg gccgtgagga tatccttgtt
     1981 gtagtagccc ttgtccagga tctccttctc cttggcgatc cccgcgacga acctcgggtc
     2041 ggtgaacttg atccggttgg cgttcaggtc atcgatcatc ctggcatccg ccttgatgtc
     2101 gtcgttccac atgatgaagg ccaggacctg gagcggccac tggtcgccgc cgcccgtgta
     2161 gatgggagcg acgttcggag ccttctgctt gatggcggcg cacacggcca gaagctcctg
     2221 gtagttcgtg gggatcttca gacccagccg ctggaacacc tgcttgttgt agaacacgcc
     2281 gtcgatgaac ggatagtcga tgatggcggc gtagtacttg ccgcggtaat tgccggctcg
     2341 gtccaggagg ccggccacgg tgttcttcac gaacggcatg tcggactggt cgatgagg
//
LOCUS       DASRZS010000229         2979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_900893, whole genome shotgun sequence.
ACCESSION   DASRZS010000229 DASRZS010000000
VERSION     DASRZS010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900893"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VFH83_05770"
     CDS             <1..718
                     /locus_tag="VFH83_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485905.1"
                     /translation="CYSPALAIRLGDQTRPPHVAAGVVTNDVNIDRDHAILVLTGPNQ
                     GGKTTYLQSVGLAQVMAQAGLFAPGSSATVSPVDAIFTHFPVEERPEFDDGRLGEEAG
                     RISLIFQQATSSSLVLLNESLASTSHGEALALAVDVVRALEASGGRAIFITHLHELAA
                     EARRLSEGNGAQSRVVSMVALTEELSGMEGSPADTVRSTFRIVEGASTGSSHASAIAR
                     RYGISYQQLLQALRSRGVIA"
     gene            complement(865..2034)
                     /locus_tag="VFH83_05775"
     CDS             complement(865..2034)
                     /locus_tag="VFH83_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6485906.1"
                     /translation="MPPTIVYITGFRQHAGKTVTSLGLLSRLRKIMDPSRLAYLKPVG
                     QELVTLPDGTQIDKDARLLQEFSGIPDFDVTSVSPVRLGSGFTQEYLDSQDHIQQTRQ
                     LEDAILSCLERLRNKEVIIAEGSGHPGVGGIVGLSNADVAGLIGAGVIFLSGGGIGKA
                     LDMLEVDLSYFLYKRIRVKGILFNKVIPNKIASMAHYITEDLLNAKYGAFGGPLRILG
                     FLPEIGDLGRPSMRTLLDRYDGAEPVWPAVGRAWETPCGQTRIITADNDALKIEEYLR
                     PGDVVVLAASSRRRAGRIIGKFRASPEGERIGGIVLTCGKTDRIDQLVRQDIAEAGMP
                     AMLVQDDTATAEQRILEIYENTKLQVYDRGKIAEIESLFAEHFDMDRFLETFRAS"
     gene            complement(2048..>2979)
                     /locus_tag="VFH83_05780"
     CDS             complement(2048..>2979)
                     /locus_tag="VFH83_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HET6485907.1"
                     /translation="GAGHLQGTINGWGERCGNMNLCTLIPTLCLKLGVQAGVCANLKQ
                     LTSLSRFVAEKANIIPDKRQPYVGDAAFSHKGGQHADVISKAPELMEHIDGAEVGNER
                     RVLLSELAGKSTIIGKLSRYGDFDKNSEIVTGLLATLKDLEEKGYEYEAAEASFDLVM
                     RRALGLYRPLLELSNYHLENYKAGETPAKTVGRIFVRVNADLLMGAAVGDGPLDTLHG
                     ALRDALLPLYPFLERLKLTDYRVRVLNPERGTAARVRVFITTSDGEETWDTVGVDENI
                     IEASWQALLDGIEYYYNTMADAGAGIATAAASS"
BASE COUNT          466 a         1020 c          963 g          530 t
ORIGIN      
        1 ctgctacagc ccggctctcg ccatccgact gggggaccag acgcggcccc cccacgttgc
       61 ggcgggagtg gtcaccaacg acgtgaacat cgaccgggat cacgccatcc tggtccttac
      121 cggacccaac cagggcggga agaccaccta cctgcagtcc gtggggctcg cgcaggtcat
      181 ggctcaggcg gggctgttcg ccccgggcag ctccgccacg gtgagtcccg tcgacgccat
      241 cttcacgcac ttccccgtgg aggagagacc cgagttcgat gacgggaggc tgggggagga
      301 ggcgggccgg atctcgctca tcttccagca ggcaacctct tccagcctcg ttctcctgaa
      361 cgagtccctg gccagcacca gccatggcga agcgctggcg ctggcggtag acgtggtccg
      421 ggccctggag gcctccggag gccgggccat cttcatcact cacctgcacg agctcgcggc
      481 cgaggcgcgc cgcctgagtg agggcaacgg ggctcagagc cgggtggtca gcatggtggc
      541 ccttacggag gagctgtcag ggatggaagg ctccccggcc gacacggtcc gaagcacctt
      601 ccggatcgtg gaaggcgcct ccacgggctc cagccacgcc agcgccattg cccgccgcta
      661 cggcatcagc taccagcagc tcctgcaggc ccttcgctct cgcggagtca tcgcttgagc
      721 cgggttcccg gctgaaacgc cggcgttgcc cgcccacgat ggccgaggcc atccgcctct
      781 accgcgctct cgggtttcgc gagatcgacc cgtacgtcca caaccccgtc cggggagcgc
      841 tctttatgga gaaggcgctg taggtcagct cgcgcggaag gtctccagga accggtccat
      901 atcgaaatgc tcggcgaaca ggctctcgat ctccgcgatc ttcccccggt cgtagacctg
      961 gagctttgtg ttctcgtaga tctcgaggat ccgttgctcc gcggttgccg tgtcatcctg
     1021 caccagcatc gcgggcatgc ccgcctccgc gatgtcctgg cgcacgagct ggtcgatccg
     1081 atcggtcttc ccgcacgtca ggacgatgcc gccgatccgc tcgccttccg gggaggcccg
     1141 gaacttcccg atgatgcggc ccgcccggcg ccgcgagctg gcggccagga cgacgacgtc
     1201 tccggggcgc aggtactctt cgatcttcag ggcgtcgttg tccgcggtga tgatccgggt
     1261 ctgcccgcac ggcgtctccc acgcccggcc gaccgcgggc cacacgggct cagccccgtc
     1321 gtaccggtcc aggagcgtgc gcatggaggg tctccccagg tcgccgatct cgggaaggaa
     1381 ccccaggatc cggaggggtc ccccgaacgc cccgtatttg gcgttcagca ggtcctccgt
     1441 gatgtagtgg gccatggagg cgatcttgtt cgggatcacc ttgttgaaga ggatcccttt
     1501 cacccggatt ctcttgtaga ggaagtagga gagatccacc tccagcatgt ccagagcctt
     1561 gccgatgcct ccgccggaga ggaagatcac gccggccccg atcagccccg ccacgtccgc
     1621 gttggacagc cccacgatcc cgcctacccc gggatggccg gacccttccg cgatgatcac
     1681 ctccttgttc cgcaggcgct ccaggcagga gaggatggcg tcctcgagct gccgcgtctg
     1741 ctggatgtga tcctgggaat ccaggtactc ctgggtgaac ccgctgccca ggcgcacggg
     1801 agagaccgac gtgacgtcga aatcaggaat cccggaaaac tcctggagca gccgggcgtc
     1861 cttgtcgatc tgggttccgt ccggcagggt caccagctcc tgtcccaccg gcttcagata
     1921 ggcgaggcgg gacggatcca tgatctttcg cagccgtgac agcaggccca gcgatgtcac
     1981 ggtcttgccc gcgtgctggc ggaagccggt gatgtagacg atggtgggcg gcacggcggt
     2041 tccctcccta ggacgacgcg gcggccgttg cgatccccgc cccggcatcc gccatcgtgt
     2101 tgtagtagta ctcgatcccg tcgaggagcg cctgccagga ggcctcgatg atgttctcgt
     2161 ccactccaac cgtgtcccag gtctcctcac cgtccgacgt cgtgatgaag acccgcacgc
     2221 gggccgcggt gcctctctcc ggattcagga cgcggacccg gtagtccgtg agcttcagcc
     2281 tttccagaaa cgggtacagc gggagcaggg cgtcccgcag cgcgccgtgc agcgtgtcca
     2341 ggggaccgtc tcctaccgcc gcgcccatca ggaggtcggc gttgaccctc acgaagatcc
     2401 gtccgacggt cttggccgga gtctcaccgg ctttgtagtt ctccaggtgg tagttgctga
     2461 gctccaggag ggggcgatag aggcccaggg ccctgcgcat caccaggtcg aaggaggctt
     2521 cggcagcctc gtactcgtaa cccttctcct ccaggtcctt gagggttgcc aggagcccag
     2581 tcacgatctc ggagttcttg tcgaaatccc cgtatcggga gagcttcccg atgatcgtgg
     2641 atttgccggc aagctctgag aggagcacgc ggcgctcgtt ccccacctcc gccccgtcga
     2701 tgtgctccat cagctcgggc gctttgctga tcacgtccgc gtgctggccc cccttgtggg
     2761 agaaagcggc atcgccgaca tagggctggc gcttgtccgg aatgatgttc gccttctccg
     2821 ccacgaaccg ggagagcgag gtgagctgct tcaggtttgc gcagaccccg gcctggaccc
     2881 cgagcttcag gcacaacgtg gggatcaggg tgcagaggtt catgttcccg cagcgctcgc
     2941 cccagccgtt gatggtgccc tgcaggtgcc ccgcgccgg
//
LOCUS       DASRZS010000230         4711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3207472, whole genome shotgun sequence.
ACCESSION   DASRZS010000230 DASRZS010000000
VERSION     DASRZS010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3207472"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="VFH83_05785"
     CDS             complement(<1..1537)
                     /locus_tag="VFH83_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET6485908.1"
                     /translation="MVTFSQINKHYGTQDVLCNVSCEILDNQKIGLIGPNGVGKTTFV
                     RLLTGEEEPSSGSIVRSPHTVIGLVPQHLDAPEGATVESYLLGGHAALANALREQEER
                     LARTPQEEIEAALREYQRARDAFDAAGGEEAPARMEAVLSGMGLPDMMRRTVATLSGG
                     ERNVLSLAKALTRRPSLLVLDEPGNHLDFAGLAWLERFLQEFDGAVLVVSHNRYLLDR
                     VVNRIFELGDNRLTQYDGNYSQYRLTRLRRLVVQQADYVANQKRLAELEALVARFAQI
                     ARTHPNPAWGRRLHARKTQLAKAQAEAVEKPHLDLRRISLGTQVERTRADIALQCNRY
                     SRGFGGTLLYQEADLTITCGERVGLVGPNGCGKTTFLRDVVERGNWDSREIRIGPSLS
                     IGYCAQHQETLDPDLTVLDQVLALGFYTRKEVFASLSRFLFTWEDLDKKVGDLSGGER
                     NRLQLARIMMLKATFLILDEPTNHMDIVSREAIEESLASFPGTILLVSHDRYLLDRIA
                     TTIV"
     gene            1920..3200
                     /locus_tag="VFH83_05790"
     CDS             1920..3200
                     /locus_tag="VFH83_05790"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HET6485909.1"
                     /translation="MRFVITPYAVAEWCAALAAVSVGVSVWKWRSSPGGTVFFLMMVA
                     AFVWAGGSGFEASVVGISGKLLFSTLAYVGTVSAPPLQLVFSLSYRRHGARPAWWVIC
                     GLWLIPAATLALAATNGAHGLVWSRLVPEPGSTIVLYEHGAWYWVSLVYDAILSLAAT
                     AVIGWTAFRAQGMYVAQAVVLLSALALPWIGVILYLTQANPFPGLELSPIAFILSAPL
                     LLLGLRRYRLLDVVPVARDLLVESMADGLIVLDDFERILDANPAARTLFGIEHEAFGR
                     NLGEVVPFLAPILPLKGKDPQTRMELAVPGDPVRHVDTRSTALGSHGSRTGYLLVIRD
                     LSERRRLELEREKLIQELKAALADIKTLEGLLPICSSCKKIRDDNGYWRTVEKYFADR
                     SEIQFTHSLCPDCVRKLFPDAAESILRDPSTAGG"
     gene            complement(3225..4298)
                     /locus_tag="VFH83_05795"
     CDS             complement(3225..4298)
                     /locus_tag="VFH83_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485910.1"
                     /translation="MAIPVQQRIDRMARWLQRENERPLIGFYVGSMYPLHLYHGSRQH
                     LSDGPVAPDDVVVEDYLEDGDRLHEIHERAGGDLLWSAAPFFGIPWVEASLGCRVVAD
                     FTTGSTRASPPPGFQDASQIPPFHESNPWIVRMLEFIPALQRRAAGRYPVGVTLMRGI
                     SDLLSALYGGEGFVFRMLEHPEDVRAAVQKLTEYWIEFGRCLLNRLPLFQGGTGSWFY
                     GLWMPGKAIWMQEDASALLSPALYEEFILPADKRIAASFEHTIVHLHPARFIPAKPLA
                     RTGIDAIELHVDHGGPTARDLLPVHREILGHKPLFIFGEIPLADIDSLLEQLPWKGLA
                     LDLVVSSVEEAHQISDHLEQVIR"
     gene            complement(4337..>4711)
                     /locus_tag="VFH83_05800"
     CDS             complement(4337..>4711)
                     /locus_tag="VFH83_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET6485911.1"
                     /translation="TFSVLRNGAKNDLRVKIGARNEASLASQYKNLWPGMTVVDITDQ
                     VRQQLGIAQDVRGVVVGYVSDQSTPAAIAGLQVGDVITAINGSTVTTIREYYQALNDL
                     SHRTVTFSLVRQGTDVSVGLTR"
BASE COUNT          807 a         1531 c         1500 g          873 t
ORIGIN      
        1 caacgatcgt ggtcgcgatc ctgtccagca gatagcgatc gtgggagacg agcagaatcg
       61 ttccggggaa cgacgccagg ctctcttcaa tggcctctcg ggaaacgata tccatgtggt
      121 tggtcggctc atccaggatc aggaaggtcg ccttgagcat catgatccgc gcgagctgaa
      181 gacgattgcg ttcgccgccg gacagatccc ccaccttctt gtccaggtct tcccaggtga
      241 agagaaagcg tgagagcgag gcgaagacct cctttcgggt atagaacccc agggccagga
      301 cctggtccag gacggtgaga tccgggtcca gggtctcctg gtgctgggcg cagtatccga
      361 tagagaggct cggaccgatg cggatctccc ggctgtccca attcccgcgc tccaccacgt
      421 cccggaggaa cgtcgtcttg ccgcatccgt tggggcccac cagcccgacc cgctcgccgc
      481 aggtgatggt caggtccgcc tcctggtaga gcagcgtccc tccgaacccg cgtgaatagc
      541 ggttgcactg cagcgcgatg tccgcccgcg tccgttccac ctgggtcccc agcgagatcc
      601 gtctcagatc gagatgcggc ttctccacgg cttcggcttg agccttggca agctgcgtct
      661 tcctggcatg gaggcgtcgc ccccaggcag gattgggatg ggtgcgggcg atctgcgcga
      721 accgggccac aagcgcctcg agctcggcca ggcgcttctg attggccacg taatccgcct
      781 gctgcaccac gagtcgccga agacgagtca atcgatactg ggagtagttg ccgtcgtact
      841 gcgtcagccg gttgtccccc agctcgaaga tcctgttcac cacacggtcc aacagataac
      901 ggttgtggga gaccaccagg accgcgccgt cgaactcctg aaggaatcgt tccagccatg
      961 ccagaccggc aaaatccaga tggttgccgg gctcatccag gaccaggagc gaaggccggc
     1021 gcgtgagcgc cttggccaac gagagcacgt tccgctctcc tccggagaga gtcgccaccg
     1081 tccggcgcat catgtcgggc aagcccatac cggacaggac tgcctccatc cgggctggtg
     1141 cttcttcgcc gcccgccgca tcgaaggcgt ctctggctcg ctggtattcc cgaagcgccg
     1201 cctcgatctc ctcctgcggc gtgcgcgcca gccgctcctc ctgctctcgg agcgcgtttg
     1261 ccagagccgc gtggccgccc agcagatagg actccaccgt ggcgccttcc ggcgcgtcca
     1321 ggtgctgggg gacaagcccg atgaccgtgt gcgggctgcg gacgatcgaa ccggaggacg
     1381 gctcctcctc tcccgtgagc aggcggacga aggttgtctt gcccactccg ttgggtccga
     1441 tcaagccgat tttctggttg tccaggatct cgcacgatac gttgcagagc acgtcttgcg
     1501 tgccgtagtg cttgttgatt tgagaaaacg taaccatggc gtctcccctt ctccgtttcc
     1561 tccgcccggc cgcttgcaga gaggggaccc gcgtgcgtcc tttcttcaga caaaaagaaa
     1621 gccgcaggct acctcttgga agcggcccgc ggcttgcctt acccgatcga gggcacgatc
     1681 agatcggccc agcggacaga cctctcccgt cggatgaagc gatgaatccg acccgagcgc
     1741 agtaggggag acaggtcatg cccgaagctc cgatcctttc acggcttgca gggtatcagc
     1801 ctgcgaggaa gccgtcaatg cgcctgcagg gcaattccag gcgccgggaa cggccgcgga
     1861 acggcgagac gaccgcgacg gcaattgccc ttgagggagg gatcgcatac gatggcgcga
     1921 tgcgtttcgt gatcaccccc tacgcggtcg cggagtggtg cgctgccctg gccgccgtga
     1981 gcgtcggggt cagcgtctgg aaatggaggt ccagccccgg aggcaccgtc ttcttcctca
     2041 tgatggtggc ggcgttcgtc tgggctgggg gctcgggttt cgaggcgtcc gtggtcggca
     2101 tttccggtaa gcttctcttt tcgacgctcg cgtacgtcgg cacggtgagc gccccgcccc
     2161 tccagctcgt cttttccctg agctatcggc ggcatggcgc gcgtcccgcc tggtgggtga
     2221 tctgcggact ctggctcatc ccggccgcta cgttggccct ggcggcaacc aacggagccc
     2281 acgggctggt ctggagccgc ctggtcccgg agcccgggtc cacgatcgtc ctgtacgagc
     2341 acggcgcatg gtactgggtg tccctcgtct acgacgcgat cctttctctc gccgctaccg
     2401 ccgtgatcgg ctggaccgcg ttccgtgccc agggcatgta cgtagcccag gcggtagtct
     2461 tgctgtccgc cctggcgctc ccgtggatcg gggtgatcct ctacctcacc caggccaacc
     2521 cttttcccgg cctggagctt tcccccatcg ccttcatcct gagcgcgcca ttgctgctct
     2581 tgggacttcg gcgttaccgg ctcttggacg tggtgcccgt ggctcgcgat ctcctggtgg
     2641 agagcatggc ggacgggctg atcgtcctcg atgacttcga gcggatcctg gacgccaatc
     2701 cggcggcgcg aacgctgttc gggattgagc acgaggcttt tggcaggaat ctgggcgagg
     2761 tcgtcccctt ccttgccccg atcctcccgc tgaagggaaa ggacccgcag acacggatgg
     2821 agctcgccgt accgggggat ccggtccggc acgttgacac ccgatccact gctctcggct
     2881 cccacggctc tcgcaccggg tacctgctgg tcatccgcga cctgtcggag cgcagacggc
     2941 tggagctgga acgagagaag ctcatccagg aactgaaggc tgccctggcg gacatcaaaa
     3001 ccctggaagg gctgcttcct atctgttcgt cctgcaagaa gatccgcgac gacaacggat
     3061 actggcgaac ggtcgagaag tacttcgcgg accgatcgga gatccagttc acccacagcc
     3121 tgtgtccgga ctgcgtgcgc aagcttttcc cggatgcggc ggaaagcatc ctgcgggatc
     3181 cgtcgaccgc gggaggctag cgccccgggc tatcgcgccc cgggctatcg tatcacctgc
     3241 tcgaggtggt ccgagatctg gtgggcttcc tcgaccgagg agactacgag gtccagggcc
     3301 agccccttcc aggggagttg ctccagcagc gagtcgatgt ccgcgagggg tatctcgccg
     3361 aagatgaaga gcggcttgtg gcccaggatc tcccgatgga ccgggaggag gtcgcgagcc
     3421 gtaggcccgc cgtggtccac gtggagctcg atcgcgtcga ttccggtacg ggcgagcggt
     3481 ttcgccggta tgaagcgagc gggatgaagg tggacgatcg tgtgctcgaa cgaggcggcg
     3541 atccgtttgt cggccggcaa gatgaactct tcgtacagcg ctggtgaaag aagcgcggag
     3601 gcgtcctcct gcatccagat tgccttgccc ggcatccaca gtccgtagaa ccaggaaccg
     3661 gtcccgccct gaaagagcgg aagccggttc agcaggcatc tgccgaactc gatccagtat
     3721 tcggtcagct tctgtaccgc ggctcggacg tcctcgggat gctccagcat ccgaaagacg
     3781 aacccttcac cgccatacag cgccgacaag aggtcgctga tgccgcgcat cagggtgaca
     3841 cctaccggat accgtcccgc ggctcgcctc tgcagagcgg ggatgaactc cagcatccgg
     3901 acaatccacg gattggattc gtgaaagggc gggatctggc tcgcgtcctg gaacccgggc
     3961 ggcggggatg cgcgcgtcga cccggtcgtg aaatcggcaa cgacgcggca gcccaacgag
     4021 gcttccaccc atgggatgcc gaagaacggc gccgcggacc aaaggaggtc gcctccggcc
     4081 cgctcatgga tctcgtgcaa ccggtcaccg tcctccaggt agtcctccac aacgacatcg
     4141 tctggagcca cgggaccatc ggacaggtgc tgccggctgc catggtagag gtgaagggga
     4201 tacatcgagc ctacatagaa cccgatgagc ggccgctcgt tctcccgctg gagccatcga
     4261 gccatgcggt cgatccgctg ctgcacgggg atagccaccc gtgcagcata ctcgcttctc
     4321 tcggctggat cgagagctag cgtgtcagtc caacgctcac gtccgttccc tgccgtacca
     4381 gcgagaacgt cacggtgcga tgggagaggt cgttcagcgc ctggtaatac tcgcggatgg
     4441 tggtgacggt cgaaccgttg atggcggtga tcacgtctcc gacctggagc cccgcaatgg
     4501 cggccggcgt gctctgatcc gagacatagc cgaccactac tccgcggacg tcctgcgcaa
     4561 tgcccaactg ctgtcggacc tggtccgtga tatccaccac ggtcatcccc ggccacaggt
     4621 tcttgtactg ggacgccagg ctcgcctcat tcctggcgcc gatcttcacc cgaagatcgt
     4681 tcttcgcgcc gtttcggaga accgagaacg t
//
LOCUS       DASRZS010000231         3362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2051961, whole genome shotgun sequence.
ACCESSION   DASRZS010000231 DASRZS010000000
VERSION     DASRZS010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2051961"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VFH83_05805"
     CDS             <1..802
                     /locus_tag="VFH83_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6485912.1"
                     /translation="LILQILDPTEGEVYFRGKPLSRMDPPELREYRRRVQMIFQDPFA
                     SLDPMMTLHEIITEPYDIFHLYTRTERRDRSADLLRRVGLDPSYGGRYPHELSGGQRQ
                     RVAIARALTLQPRAIIADEPTSALDVSVKAQIINLLEKLRSEEGLAIVFISHDLSMVQ
                     HISDRIIVMYLGKIVESGPTAQIFDAPSHPYTRALLDAIPRPDPRRRRARVAIRGEAV
                     GGLGIERGCVFYPRCSLRTAECLAAQPPLVETAPGRNVACIHPLGYRE"
     gene            813..1748
                     /locus_tag="VFH83_05810"
     CDS             813..1748
                     /locus_tag="VFH83_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485913.1"
                     /translation="MALYILKRVWSLLVSFLVVSVVIFAMMHSVPGGPFDPTYMPLSD
                     AAKHLILQKYGLDKPLYVQYLKYMWSFVRGDFGIPYQSPGETVTDLLARAWPPSLLLG
                     GLGMVLGTPIGMLLGIAAAVRRNSIIDYIASLISTIGISVPIFVTSLILMLLFSVWLH
                     WLPSNGWGGPVNWILPIASYSVVPIATFARYTRSTILDSLNKPFVVVLRSKGLSRWSV
                     VMKHVLKNAAIPMITIFFPMFIGIATGTVFVEKMYRVPGLGNYFVSSIFARDYPMEMA
                     LALVLTLLVGAAYMITDILYTLLNPTIRLHAGRLR"
     gene            1745..2605
                     /locus_tag="VFH83_05815"
     CDS             1745..2605
                     /locus_tag="VFH83_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485914.1"
                     /translation="MTPRSHGPLFYAFERFRDNKAAVGAGIVLGIIVLSAIFAPLLTP
                     TGPNEQAYLVRILGFPSAQNWFGVDAVGRDLYSRIVFGARVSVGVGTVSALVSLVIGV
                     PLGALAGYRGGKADWVVMRFVEIFSVIPPLLVALLVAALVGGGVVNVVLIASAFGWVQ
                     VCRLVRGQVMAQRQQTFVVAARAMGASPWYLIRRHLIPNSISPIIIGFVLAIPQAMML
                     EAMLSFLGAGIRPPIPSWGQMISDGLYYMFHYWHLAVFPTLALAVTVLSTSVFGDGLR
                     DALDPTLKGR"
     gene            complement(2695..>3362)
                     /locus_tag="VFH83_05820"
     CDS             complement(2695..>3362)
                     /locus_tag="VFH83_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485915.1"
                     /translation="GSNDATRARYNGSLKIGLASSTDGTHWTKHGVVLDGTSPSAAPL
                     PGQGRIRAVDGWRHVGYCGWVTRQDDTYVMLFNDNRFEFNISRATASAPTGPWTLDPA
                     NPVLTGTATPWANKLEDCTVVRAGSWWLVFVDSLGRSLPGAPCGLPVYRTRDLIRGPF
                     ELFELFVPLPDSRGLGRFDSDSIGTPGACLTPDGRILLMYSGPQDQDGAEIGAVFIEI
                     PGD"
BASE COUNT          541 a         1078 c         1068 g          675 t
ORIGIN      
        1 cctcatcctg cagatcctgg atccgaccga gggtgaggtg tacttccgcg gaaagcctct
       61 gtcccgaatg gatcctcccg agctgcggga atatcgccgg cgggtgcaga tgatcttcca
      121 ggacccgttt gcgtccctgg accccatgat gaccttgcat gagatcatca cggagcccta
      181 cgacatcttc cacctgtata cccggaccga gcgccgggac cgaagcgcgg atctgttgcg
      241 gcgggtgggg ctggatccca gctatggcgg ccgctatccc cacgagcttt ccgggggcca
      301 acgtcagagg gtggccatag cccgggcgct gaccctccag ccgcgagcca tcatcgcgga
      361 tgagccgaca tcggccctgg acgtgtcggt gaaggcgcag atcatcaatc ttctggagaa
      421 gctcagatcc gaggaaggtc tggccatcgt cttcatctcc cacgacctga gcatggtcca
      481 gcacatcagc gaccggatca tcgtcatgta cctggggaag atcgtggagt cggggccgac
      541 ggctcagatc ttcgatgcgc cgtctcaccc ctacacccgg gccctgctgg acgccatccc
      601 gcgtccggat ccccgcagac ggcgcgcgcg cgtcgccatt cgcggagagg cagtgggtgg
      661 cctggggatc gagcggggct gcgttttcta tccgcgctgc agcctgcgaa cggcggagtg
      721 cctggcggcc cagccccccc tggtggaaac ggcgcctggc agaaacgtgg cgtgcatcca
      781 ccctctggga taccgggagt gaggtgaacg agatggccct ctacatcctc aagcgcgtct
      841 ggtctctgct ggtgtccttc ctcgtcgtat cggtggtcat cttcgcgatg atgcactcgg
      901 ttcccggagg gccgttcgac ccgacgtaca tgccgctctc cgacgcggca aagcacctga
      961 ttctgcagaa gtacggtctg gacaagccgt tgtatgtcca atacctgaaa tacatgtgga
     1021 gcttcgtccg aggggatttc ggcattcctt accagagccc cggggagacg gtcaccgatc
     1081 tgctggcaag ggcgtggccg cccagtctcc tgctgggagg gctcggcatg gtcctgggca
     1141 cgccgatcgg aatgctcctt gggatcgccg ccgccgtgcg ccgcaacagc atcatcgact
     1201 acatcgcgtc gctcatctcc accatcggga tcagcgtgcc catcttcgtc acgagcctga
     1261 tcctcatgct gctcttctcg gtctggctgc actggctccc cagcaacggg tggggagggc
     1321 ccgtgaactg gattctgcct atcgcctcct actccgtggt tcccatcgcg acctttgcgc
     1381 gttacacgcg gtcgaccatc ctggacagtc tgaacaagcc gttcgtcgtg gtcctgagat
     1441 ccaagggact gtctcggtgg tccgtggtga tgaagcacgt tctcaagaac gccgccattc
     1501 cgatgatcac catcttcttt cccatgttca tcgggattgc gacgggtacg gtcttcgtcg
     1561 agaagatgta tcgggtgccc gggctcggca actatttcgt ctcgagcatc tttgcccggg
     1621 actatccgat ggagatggcc ctcgccctgg tgctgaccct gctggtgggg gccgcctaca
     1681 tgatcaccga tatcctctac accttgctca acccgacgat tcggctccat gcgggaaggc
     1741 tgagatgacg cccagatccc acgggccgct tttctatgcc ttcgagcgat tccgcgacaa
     1801 caaggctgcc gtgggagccg gaatcgttct gggcatcatc gtcctgagcg ccatcttcgc
     1861 tcccttgctg accccgaccg gcccgaacga acaggcgtat ctggttcgga ttctgggatt
     1921 ccccagcgcg cagaactggt tcggtgtcga cgccgttgga agagacttgt actcccggat
     1981 cgtcttcggc gcacgggtat ccgtgggcgt ggggaccgtg tccgccctgg tcagccttgt
     2041 catcggcgtg ccgctgggag cgctggccgg ctaccgcgga ggcaaggccg actgggtggt
     2101 gatgaggttt gtggagatct tctccgtcat cccgccgctc ctggtagccc tcctggtggc
     2161 ggcgctcgtg ggcgggggtg tggtcaatgt cgtcttgatc gcttctgcgt ttgggtgggt
     2221 ccaggtgtgc cgtctggtcc ggggtcaggt gatggcgcaa cgccagcaga ccttcgtggt
     2281 ggctgcccga gccatgggcg ccagtccgtg gtaccttatc cgacgtcacc tcatccccaa
     2341 cagcatttcg cccatcatca tcggcttcgt gctcgcgata ccgcaagcca tgatgctgga
     2401 ggccatgctg agctttcttg gcgcgggcat tcggcccccc ataccaagct ggggccagat
     2461 gatcagcgac gggctatact acatgttcca ctactggcac ctggccgtct tcccgacgct
     2521 cgcgctggcc gtcaccgttt tgtccacctc tgttttcggc gacgggttgc gggatgcgct
     2581 ggatccgacc ctcaagggcc gatagccgac ttttccatcg ggatggagcg gccggtccgt
     2641 ccgaacgagg acgggagcgg ctgcaattcc taggtatccc tggtgtccgt gttctcagtc
     2701 cccggggatc tcgatgaaca cggcgccgat ctcggcgcca tcctgatcct gaggcccgct
     2761 atacatcagc aggattctcc cgtcgggcgt gaggcacgcg cccggagtgc cgattgaatc
     2821 gctgtcgaaa cggcccagcc cccggctgtc cggcaggggc acgaaaagct cgaacagctc
     2881 gaatggacct cggatgaggt cccgggtgcg atagacggga aggccgcagg gtgcgcccgg
     2941 cagcgagcgc ccgaggctgt ccacgaacac gagccaccag gaacccgcgc ggacgaccgt
     3001 gcagtcctcc agcttgttcg cccagggcgt tgccgtgccg gtgaggaccg ggttcgccgg
     3061 gtccagcgtc cagggaccgg tgggcgctga cgccgtggcg cggctgatgt tgaactcgaa
     3121 ccggttatcg ttgaaaagca tgacgtaggt atcgtcctgc ctcgtgaccc atccgcagta
     3181 gccgacatgg cgccagccat ccacggcgcg gatacggccc tgcccgggaa ggggcgccgc
     3241 gctcggtgac gtgccatcca ggacgacgcc gtgcttcgtc caatgggtgc cgtccgtaga
     3301 ggaggcgagg ccgatcttca gagacccgtt gtaccgcgct ctggtggcgt cgttggagcc
     3361 gt
//
LOCUS       DASRZS010000232         7309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5518449, whole genome shotgun sequence.
ACCESSION   DASRZS010000232 DASRZS010000000
VERSION     DASRZS010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5518449"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFH83_05825"
     CDS             <1..235
                     /locus_tag="VFH83_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485916.1"
                     /translation="ADVRLVRDSVELFKGKIASLRRFKDDAREVEEGFECGIGLENFH
                     DIKVGDTLEVYETRELAKKLEGSRDGGSKAPAT"
     gene            264..554
                     /gene="rbfA"
                     /locus_tag="VFH83_05830"
     CDS             264..554
                     /gene="rbfA"
                     /locus_tag="VFH83_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET6485917.1"
                     /translation="MIFSGEIKDPRVERLTTVTEVNVSRDLGHAKVWVSRYGDREAVS
                     ASVDALNHAAGFIQGVLSKRISVRTFPRLTFLRDDSMENGFRIAKKLRELMH"
     gene            551..1453
                     /gene="truB"
                     /locus_tag="VFH83_05835"
     CDS             551..1453
                     /gene="truB"
                     /locus_tag="VFH83_05835"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET6485918.1"
                     /translation="MTDGIVLLDKPAGLTSFQCLDRIKRALGTRRVGHAGTLDRFAEG
                     LLIALTGTMTRLCPFASEMDKEYVAEVTFGRGTDTLDPEGATTAEGRVPDAGEIAEAL
                     GRFRGPILQSPPAYSAVHIDGQRAYQAARKGQTVVLAARPVTVHALELSGFTPPRAVL
                     RIVCSKGTYVRSLARDIAEVIGTCAYVSRLRRTRSGGFLVGQAVRPDSFDSNRDLLPP
                     RAFFDAAPGLGTLEVEEGTARKVRHGVPLDDRDFAMPPGREGPFGVFSRGGHLLAVVE
                     RRAGAFRYSAVFSEAADTDPGEPR"
     gene            1450..2226
                     /locus_tag="VFH83_05840"
     CDS             1450..2226
                     /locus_tag="VFH83_05840"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HET6485919.1"
                     /translation="MRLIPWAKLGRPGWEVPVPVRLTIGVFDGVHLGHRRLIRGILDD
                     AAGRLPLVVTFEQNPQQLLAPGSFPGSITTYAQKLERLEALGVQAVVAIDFSNELSKL
                     SGKAFLGALRENLTIEKIVVGYNFRYGKGRSAGTDDLRETFAGTGTDVVVAEPVLWGD
                     SAVSSSRIRKTVQEAELAAAREMLAAPFSVDLRGLSLETLGSGCFLVQRRILSQILPP
                     IGTYRVSCEGEAGTGRVTVTVDDLVLEGTLAWDTRPIAFD"
     gene            2262..2531
                     /gene="rpsO"
                     /locus_tag="VFH83_05845"
     CDS             2262..2531
                     /gene="rpsO"
                     /locus_tag="VFH83_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET6485920.1"
                     /translation="MPLTKEAKLEVVKTFGVSEKDTGNTEVQVALLTRRVEELTEHFK
                     RHPKDNNSRRGLLKLVGQRRRLLGYLRRRNLTKYREIIERLNLRK"
     gene            2747..5017
                     /gene="pnp"
                     /locus_tag="VFH83_05850"
     CDS             2747..5017
                     /gene="pnp"
                     /locus_tag="VFH83_05850"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET6485921.1"
                     /translation="MEQRVSLRIGEEELILETGKMAKQANGAVFARLGGSAVLATACC
                     ADREKEDLDFIPLSVDYIEKYYAAGKIPGGFIKREGRPKDKEVLVSRLIDRPIRPLFS
                     RLFRREIQVIPIAVSTDQVNPPDIVAMNAASAAITVSDIPFEGPIGAVRVGYVDGALV
                     INPSFDQIDRSTVELVVAGTEKGITMVEGGASQVGESLLIEAIQMAHVTIKELCRIQR
                     ELARTAGKIKLPLVERPSTFTLGSEMRAWAQDRMEKACFVKGKMERSAAIKEVREAAK
                     VQFADRLAETDIRPVHALFEDMDQQILRKSVVERGIRTDGRRPDEIRPITCEVDVLPR
                     THGAALFTRGETQALVVTTLGTALDEQIMDNIDGDSKKRFMLHYNFPPFSVGEVGRLS
                     SPGRREIGHGHLAERAIERVLPSKEVFPYTVRIVSEILESNGSSSMATVCGSTLSLLN
                     AGVRILKPVAGIAMGLVKEGERYVVLSDILGEEDHLGDMDFKVAGTEDGVTALQMDIK
                     IEGVSAEILTKALEQARQGRLFILSVMGKTISQPRTDISDFAPKVISIKVPTEKIGLI
                     IGPGGKNIKAMSEKSGSTINIDDDGTVTIYNHQKDGAEKAQRMVEALIEEAVVGKVYT
                     GPVKRIMDFGAFVEILPGKEGLVHISRLAATRVNAVTDVVHEGQELTVKVTEIDHMGR
                     INLATLEALEANPDLATERPERRSPPRSYGGSRGGPRGSDRRPGGFSDRPPRRDGGHD
                     RPRGPSSGPRPPRREG"
     gene            5014..6315
                     /locus_tag="VFH83_05855"
     CDS             5014..6315
                     /locus_tag="VFH83_05855"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6485922.1"
                     /translation="MIHTEKLASGATLLLEPVDRTDTLCIGFWFLHGSRDESPAERGY
                     SHFLEHMLFKGTSRRSALAIAQEIDRVGGLINAFTEKEATCVYVILPKEHLRLAFDVL
                     SDMTVDSVLDPAEMEKEKAVIVNEIRSVDDSPEEKGHDRFLVETLGDHPLSRKITGEV
                     EEVQSIDRPSLERFYRQRLVGANTVIAVAGNCDPSEVRDLAERVFPSASPASLPKRSL
                     PAWTRRVSVVPDRFNQAQIYAGTAYPLDHDIGHYYTSLVFSTAFGESMSCRLFQRLRE
                     ELALCYTVYSFRSFFSDTGMWTIYANATPAQTRTFLGALDAELCRLLAEPLSEKEISD
                     AKSHLGGSMILSREDMETRMKRLARQFTMMERILTFEESVAALQRVTTEEAAAFARRC
                     LGPDTFSLLTYGCRGISNVRGFDFSFQAAGVAPSARRRGPG"
     gene            6325..6762
                     /gene="dut"
                     /locus_tag="VFH83_05860"
     CDS             6325..6762
                     /gene="dut"
                     /locus_tag="VFH83_05860"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET6485923.1"
                     /translation="MDEPVVFCVTRSGSLPEYRSAGAAGADLRASLEKEIVLSPGERR
                     LVPTGLSAAIPEGWEGQVRPRSGIAVRYGVTVLNAPGTIDSDYRGEIQVLLVNLGQED
                     FVIRSGDRIAQLVIAPTSRAVFQARESIDETERGSGGFGSTGT"
     gene            6716..>7309
                     /locus_tag="VFH83_05865"
     CDS             6716..>7309
                     /locus_tag="VFH83_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485924.1"
                     /translation="MKPKEGAEALARPGRDRILSLLTAAVLMGVVTGSPASAQAASAD
                     PAAAMVDAARQAGARGAASEGLDDLRAMRSRYPDSPLIAASYAVGVDLALSSGDLYLA
                     RFYMDRLYAIAPSGVDTFRRAQAIASYYYQRKSYAEALPYYRQAVLSGGAAGTAAATA
                     PGVSAPDLATVRLRAAELDAYHQGDEQEARRFFSAISR"
BASE COUNT         1316 a         2309 c         2442 g         1242 t
ORIGIN      
        1 cgccgacgtc cggctggttc gagactcggt ggagttgttc aaggggaaga tcgcatccct
       61 gcgcaggttc aaggacgacg ccagggaggt cgaagagggg ttcgagtgcg gcataggcct
      121 ggagaacttc cacgacatca aggtaggcga caccctggag gtctacgaga cccgcgagct
      181 ggccaagaag ctcgaaggga gccgggatgg cggaagtaag gctccggcga cttgagagcc
      241 tcctccggga ggagatctgc tccatgatct tctccggaga gatcaaggac cctcgggtgg
      301 agcgtctgac gaccgtgacg gaggtcaacg tcagccgcga cctcggacac gcaaaggtct
      361 gggtctcccg atacggagac cgtgaggctg tgtcggcgag cgtggatgcc ctcaaccacg
      421 ccgccggctt catccagggc gttctctcca agcggatatc ggttcgcacc tttccccggc
      481 tgacgttcct ccgggacgac tcgatggaaa acggtttccg gatcgccaag aagcttcggg
      541 aactcatgca ttgacggatg gcatcgttct tctcgacaag cccgcaggcc tgacatcctt
      601 tcaatgcctc gaccgaatca agcgcgccct gggaacgcgt cgggtcggcc atgcggggac
      661 gctggaccgg ttcgccgaag ggcttctcat cgcgctgacc gggacgatga cccggctgtg
      721 tccgtttgct tccgagatgg acaaagagta cgtggcggag gtgaccttcg gcaggggcac
      781 agacaccctg gatccggaag gcgcgacgac ggcggagggc agggtccccg acgcgggtga
      841 gatcgcggaa gctctaggcc gcttccgggg acccatcctt cagtccccac cggcttactc
      901 cgccgtgcac atcgacgggc agcgcgccta ccaggcggcc cgaaagggac agacggtggt
      961 attggcggcc cggcccgtca ccgttcatgc gctcgagctc tccgggttca cgccaccccg
     1021 ggccgtcctt cggatcgtgt gctccaaagg gacgtacgtc cgctcgctgg cgcgcgacat
     1081 cgctgaagtg atcggcacct gcgcctatgt ttccagactg cgtcggaccc gtagtggcgg
     1141 attcctggtg ggtcaggcgg ttcgcccgga ttccttcgac tcgaatcggg atctccttcc
     1201 gccgcgcgcc ttcttcgacg cagcgcccgg tcttggcacc ctcgaggtag aagagggcac
     1261 cgcgcgcaag gtccgtcacg gcgtccctct cgacgatcgc gacttcgcga tgcctcctgg
     1321 ccgggaaggg ccgttcggtg tgttctcccg gggcgggcat cttctggccg tggtggagcg
     1381 aagggccggt gcattccgct actcggcggt gttctccgaa gcggccgaca cggatccggg
     1441 ggagccgcgg tgaggttgat cccctgggcg aagctgggac gccccggctg ggaggttccg
     1501 gtcccggttc ggctcaccat aggggttttc gacggggtgc accttgggca ccgacggctc
     1561 atccgaggga tcctcgacga tgccgcgggg cgattgcccc tcgtagtcac cttcgagcag
     1621 aatccgcagc agcttcttgc gcccggttcg tttcccggct ccatcacgac ctatgcgcag
     1681 aagctcgagc ggctggaagc gctcggcgtc caggcagtcg tagcgattga cttttccaat
     1741 gaattgagta agctttccgg aaaggcgttc ctgggcgctc tgagggaaaa cctcaccata
     1801 gagaagatcg tcgtcgggta caactttcga tatgggaagg ggaggagcgc cggaactgac
     1861 gacttgagag agacgtttgc cggcacgggg acggatgtgg ttgttgccga gccggtgctt
     1921 tggggcgaca gcgccgtgtc gagctcccga atacggaaga cggtgcagga agcggagctc
     1981 gccgccgcgc gagagatgct cgcagccccc ttcagcgtgg atctgcgtgg gctttcgctg
     2041 gagacgttgg gctccggttg cttcctcgtc cagcgacgaa tcctgagtca gattctgcca
     2101 cccatcggca cgtaccgcgt atcgtgtgag ggagaggccg gaacgggacg cgttacggtg
     2161 accgtcgacg accttgtgct ggaggggacg ctggcctggg acacccggcc aatagcattc
     2221 gactgaaaac tggttcgaaa cgatcggaag gagtaccgag aatgccactg accaaggaag
     2281 ccaagctgga agtcgtgaag acgtttggcg taagtgagaa ggacacggga aacacggagg
     2341 tgcaggtcgc tctcctgacc cgccgcgtag aggagctcac ggagcacttc aagcggcatc
     2401 ccaaggacaa caattcgcgg cgcggtctcc tcaagctcgt cggacagcgg cgtcggttgc
     2461 tcgggtatct tcgccggagg aacctcacga agtaccgcga gatcatcgaa cgcttgaacc
     2521 ttcgcaaata ggcgcgcccg tttgtcaatg aacgtcgtgc gcacggtcga cgcagctctg
     2581 cgggaggggt ctgaccgccc cctgcggcag ctcaccgcgc gcgctgagcg acgccgatag
     2641 gatgcgccgg acggccggcg cggaatgcgc gcaagagcgc gcggactgaa gtgcaccggg
     2701 tacggtgcag attcgaatgc accgacacgg tgcagaggta ggagcaatgg aacaacgagt
     2761 gtcattgaga atcggagagg aggagctcat cctcgagacg ggcaagatgg cgaaacaggc
     2821 aaacggtgcc gtgttcgccc ggctcggcgg atccgcggtg ctcgccaccg cgtgttgcgc
     2881 ggatcgtgag aaggaagacc tcgacttcat acccctctcc gtcgactaca tcgagaagta
     2941 ctacgccgcc gggaagatcc ctggaggatt catcaagcgc gagggaagac ccaaggacaa
     3001 ggaggtcctg gtctcccgct tgatcgaccg tccgatccgg cccctgtttt ccaggctgtt
     3061 ccgccgggag atccaggtca tccccattgc ggtctccacg gaccaggtga acccgccgga
     3121 catcgtggcc atgaacgcgg cgtccgcggc cattaccgtt tccgacatcc ccttcgaggg
     3181 gcccatcggc gcggtgcggg tcggttacgt ggatggagcg ctcgtgatca atcccagctt
     3241 cgatcagatc gatcggagca ccgtggagct ggtggtggcc ggtacggaga aggggatcac
     3301 gatggtggag ggcggggcca gccaggtcgg cgagtccctt ctgatcgaag ccatccagat
     3361 ggcccatgtc accatcaagg agctgtgccg gatccagcgg gagctcgccc gcacggcggg
     3421 caagatcaag cttcccctgg tggaacggcc ctctaccttc acgctggggt ccgagatgcg
     3481 ggcctgggcg caggaccgga tggagaaagc ctgtttcgtc aaaggcaaga tggagcgctc
     3541 cgctgccatc aaggaagtcc gtgaggcggc caaggtccag tttgcggacc gattggcaga
     3601 gacggacatc cgaccggtgc acgccctgtt cgaggatatg gaccagcaga tcctgaggaa
     3661 atccgtcgtc gaacgcggga tccgtacgga cggcagaagg ccggatgaga tccgcccgat
     3721 cacctgtgag gtcgacgtgc ttccccgcac ccacggtgcc gcgctcttca cgcgcggaga
     3781 gacccaggcg cttgtggtga ccacgctcgg cacggcgctg gacgagcaga tcatggacaa
     3841 catcgacgga gacagcaaga agcgcttcat gctccactac aacttcccgc cgttctccgt
     3901 cggcgaggtg ggacgattga gctctccggg ccgccgggag atcgggcacg gtcacctggc
     3961 cgagcgcgct atcgaacggg ttctcccgtc taaagaggtc tttccgtaca cggttcggat
     4021 cgtgtcggag atcctggagt ccaacggctc ctcttccatg gcgaccgtct gcggcagcac
     4081 gttgagcctg ctgaacgcgg gcgttcggat cctgaagccg gtggcgggca tcgccatggg
     4141 actggtcaag gaaggcgagc ggtatgtcgt cctcagcgac atcctcggtg aggaagacca
     4201 cctgggagac atggacttca aggtggcggg aacggaggac ggggtgaccg ccctccagat
     4261 ggacatcaag atcgaaggtg tcagcgcgga gatcctgacc aaggctctcg agcaagcgcg
     4321 acagggacgg ctctttatcc tttccgtcat gggcaagacc atttcccagc cccgcaccga
     4381 tatctcggac ttcgccccca aggtgatctc gatcaaggtc ccgacggaga agatcgggct
     4441 catcatcgga ccgggcggga agaacatcaa ggcgatgagc gagaagtccg gctccacgat
     4501 caacatcgac gacgacggga ccgtcaccat ctacaaccat cagaaggatg gggccgagaa
     4561 ggcgcagcgg atggtggagg ccctcatcga agaggccgtg gtgggcaagg tttacaccgg
     4621 gcccgtcaag cggatcatgg atttcggggc tttcgtcgag atcctccccg gcaaggaagg
     4681 cctggtccat atctcccgcc ttgcggcaac gcgggtcaac gccgtgacgg acgtggttca
     4741 cgagggccag gagctcaccg tcaaggtcac ggagatcgac cacatgggca ggatcaacct
     4801 ggccacgctg gaggccctgg aagccaaccc ggacctcgcc acggaacggc cggagcgccg
     4861 gtccccccct cggtcgtacg gcggttcccg gggcggccct cgcggatccg accgcagacc
     4921 cggcggattc tccgatcggc ctccccggcg ggacggcggt cacgaccgcc cgcgcggacc
     4981 gtccagtggc ccgcggccgc cacggagaga agggtgatcc acaccgagaa gctggcaagc
     5041 ggagctacgc tgctcctgga gccggtggac cggacagata ccctctgcat cggcttctgg
     5101 tttctccatg ggtcccggga cgagtccccc gccgagcggg ggtactcgca tttcctggag
     5161 cacatgctct tcaagggcac ttcgcgaagg agtgccctcg ccatagcgca ggagatcgat
     5221 cgggtcggcg gcctgatcaa cgccttcacc gagaaggagg caacctgcgt ctatgtcatc
     5281 ctccccaagg agcatctgcg gctggccttc gacgtgctct ccgacatgac ggtggactcc
     5341 gtgctggatc ccgccgagat ggagaaggag aaggccgtca tcgtcaacga gatacgctcg
     5401 gtagacgatt ccccggaaga gaagggccac gaccggttcc tggtcgagac gttgggtgac
     5461 catcccctgt cccggaagat caccggcgaa gtcgaagagg tccagagcat cgaccgacca
     5521 agcctggagc ggttctaccg ccagcgcctg gtaggggcca acacggtcat cgcggtggcc
     5581 ggcaactgtg acccgtcgga ggtgcgggac ctcgcggaga gagtcttccc gtccgcgtct
     5641 ccggcctcct tgcccaagcg ctctctgccc gcctggaccc ggagggtgtc ggtagtgccg
     5701 gatcggttca accaggccca gatctacgcc ggcacggcct atcccctgga ccacgacatc
     5761 ggccactact acacgtccct cgtcttcagc accgctttcg gtgagtccat gagctgccgg
     5821 ctcttccaga ggctgcggga agagctggcc ctgtgctaca ccgtctactc gttccgttcg
     5881 ttcttctcgg ataccgggat gtggacgatc tacgccaacg ccacgccggc ccagaccagg
     5941 acgttcctgg gtgccctgga cgccgagctg tgccggcttc tcgccgagcc gctctcggag
     6001 aaggagatct cggacgccaa gagccacctc ggtggcagca tgatcctctc ccgagaggac
     6061 atggagaccc ggatgaagcg gctggcccgt cagttcacca tgatggagcg gatcctgacc
     6121 ttcgaagaat cggtagcggc gctccaacgg gtgaccacgg aggaggcggc tgcgtttgcg
     6181 cggcgctgcc tcggccccga caccttcagc ctgctgacct acggctgtcg ggggatctca
     6241 aacgtccgtg gcttcgactt cagtttccag gccgcgggcg tcgctccgtc cgcgaggcgg
     6301 cgcggcccgg ggtgacagac cgccatggac gaacccgtcg tcttctgcgt gactcgaagc
     6361 gggtcgctcc ccgagtaccg ctctgccggc gccgccggag ctgacctcag ggcgtcgctg
     6421 gaaaaggaaa tcgtgctgag ccccggcgag cgccgtctcg tcccgaccgg actttcggcg
     6481 gcgattcccg agggatggga gggtcaggtc cggccccggt ccgggattgc ggtccgctac
     6541 ggtgtgacgg tgctcaacgc ccctgggacg atcgacagcg actaccgagg cgagatccag
     6601 gtcctgctgg tcaacctggg gcaggaagac ttcgtgatcc gctcgggcga ccggatcgct
     6661 cagctcgtga ttgctccgac ctctcgcgcc gtgttccagg cgcgggagtc gatcgatgaa
     6721 accgaaagag ggagcggagg ctttggctcg accgggacgt gaccgcatcc tatcgctgct
     6781 gacggcggcg gtcctcatgg gcgtcgtcac ggggagcccc gcttcggcgc aggcggcctc
     6841 cgcggatcca gccgcggcca tggtggatgc cgcgcgccag gccggggccc gcggggctgc
     6901 ctccgagggt ctggacgact tgcgcgccat gcgatcccgc taccccgatt cgcccctgat
     6961 cgccgcctcc tatgccgtcg gagtcgacct ggcgctctcc tcgggagatc tctacctggc
     7021 tcggttctac atggatcgcc tctatgccat tgcccccagc ggggtcgaca ccttccgcag
     7081 ggcccaggcg attgcctcct actactacca gcggaagtcc tacgcggagg ctttgcccta
     7141 ttaccggcag gcagtgctat ccgggggcgc tgccggcacg gccgccgcga ccgccccagg
     7201 ggtctccgcg ccggacctgg ccacggtccg cctgcgcgcc gccgagctcg acgcgtacca
     7261 ccagggagac gagcaggagg ctcgtcggtt cttttccgcg atctctcgc
//
LOCUS       DASRZS010000233         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4362856, whole genome shotgun sequence.
ACCESSION   DASRZS010000233 DASRZS010000000
VERSION     DASRZS010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4362856"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..795
                     /locus_tag="VFH83_05870"
     CDS             115..795
                     /locus_tag="VFH83_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485925.1"
                     /translation="MLLTAMLIVAAAGAFGQDLRISAGLDAGFVPTVYENQTVADSAG
                     TCFPAGTQLREDYSYSAARMGAFVDLTHGILSVAYRTSVTPLNASISVLGQTASGQAA
                     LSLSALETRALVKIPFQMVGLTVLVLAGPEYTACLSGSIGGIALDDASKQDLSDFSLV
                     GGFGVDLVSGEKWFLRSSVMAGYSLTSKRSSQYYAGVAYVSSSRWRLEIDLGAGWLVV
                     GRNAAERH"
     gene            839..1408
                     /locus_tag="VFH83_05875"
     CDS             839..1408
                     /locus_tag="VFH83_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485926.1"
                     /translation="MNRSWLLAGLAGLIGAGLLLVSCASIPKPETESDTLVVGSFAVD
                     YPDGFYNLPPRTLVSNIRLDFMNRSTGKEFWVITDFDGRYSFLSSGGQSYELKSFAYD
                     LRDGNTSFSGSSSISYRFYAPAHSVLYLGDIHFREEKPTQKTTVSRETYWTFTDSISR
                     ADKSDEMKTYLQKAAKDTPWPSYVFVRAS"
     gene            1502..2248
                     /locus_tag="VFH83_05880"
     CDS             1502..2248
                     /locus_tag="VFH83_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485927.1"
                     /translation="MPSIIRGPRLRALLGLVPLLFALVVCACSVSQTVVVKSDGSGTL
                     TMHVEMSTVLHDYIASLSEATGQKAQSPSGQVFNPDDIRKSFAAQQGITVQKVSSPSP
                     NILDVSLAFTSLGDVFSQNKSIKGTGALLYTDTEGTRTIRLHLDRTNYTQLSAVFPLL
                     ADPMIASMGPQVNDKISDSEYLEMIKFTLGDEAPGLVQKSFVTITIKPEGQILSQTGG
                     TLSGGAVVFKIPLLRLLVLDKPLDYTVSYK"
BASE COUNT          417 a          734 c          699 g          433 t
ORIGIN      
        1 tcgtcatggt aggctagcat catcgccgtg atccaaaacg tcgattcaga atgccgaaag
       61 gcaccggctc gccctttggt gttcgtcggc gagccggcgt cttttcgccg gaaggtgctc
      121 ctgacggcga tgttgatcgt agcggcggcc ggcgccttcg ggcaggacct gagaataagc
      181 gctgggctcg acgctggctt cgtccccacc gtctacgaga accagaccgt ggcggattcg
      241 gccggcacct gctttcccgc gggtactcag ctccgcgagg actacagcta cagcgcggcg
      301 cggatgggtg cgttcgtcga tctcacccat gggatcctct cggtggcata tcggacctcc
      361 gtgactccgc tcaacgccag catctccgta ctgggccaga cagcctcggg ccaggcagca
      421 ctgtccctct ctgcgctgga gacgcgggca ttggtgaaga ttccattcca gatggtcggg
      481 ctgaccgtcc tggtcctggc cggcccggaa tacaccgcct gcctctccgg ctcgatcggc
      541 ggcatagcgt tggacgatgc gtcgaaacag gacctctccg acttctccct cgtcggaggc
      601 ttcggagtgg acctcgtctc gggcgagaag tggttcctgc ggtcgtccgt gatggctggc
      661 tacagcctca ccagcaaaag gagttcgcag tactacgcag gcgttgccta cgtgagctca
      721 tcgcgttgga ggttggaaat cgatctcggc gcaggatggt tggtcgtcgg cagaaacgcg
      781 gcggagcggc actgaggctg acctccagcg cgccgcggcc cgacagggag gagtggaaat
      841 gaaccgaagc tggttgctgg cgggactggc gggcctgatc ggtgccggac tcctgctcgt
      901 ctcctgcgcg agcatcccca agccggagac ggagtcggat acgctcgtgg tgggctcctt
      961 cgcggtggat tacccggatg gtttctacaa cctgccccct cggacgttgg tgtcaaacat
     1021 tcgattggac ttcatgaatc gcagcaccgg caaggagttc tgggtcatca cggatttcga
     1081 cggccgctac agcttcctta gctccggcgg ccagagctac gagctcaaga gcttcgcgta
     1141 cgacctgcgg gacggcaaca cctcgttctc gggctcctcc tcgatctcgt accgcttcta
     1201 tgctccagct catagcgtcc tgtatctcgg cgatattcac ttccgcgagg agaagcccac
     1261 gcagaagacg acggtctccc gggagacgta ctggaccttc accgactcga tcagccgagc
     1321 agacaagtcg gacgagatga aaacgtatct gcaaaaggcg gcgaaggata ccccttggcc
     1381 ctcctacgtc ttcgttcggg catcctgagc cgctgggagc cagcaggagc tccgatccgc
     1441 gagtagcgcc ccgcatcccc tggggttgac gcgccacgaa gcgcggcata ccgtgtcgcg
     1501 catgccatcc atcatccgtg gcccgaggct ccgagcgctc ctcggtcttg ttccgctcct
     1561 ctttgcgctc gtcgtttgcg cgtgctcggt cagccagacg gtcgtcgtga agagcgacgg
     1621 ctccggaacg ctgacgatgc acgtcgagat gtccacggtc ctgcacgact acattgccag
     1681 tctctccgaa gccacggggc agaaagccca gagccccagc ggccaggtct tcaatcccga
     1741 tgacatcagg aagagctttg ccgcccaaca ggggatcacg gttcagaagg tcagctctcc
     1801 gagcccgaat atcctggacg tgagccttgc cttcacctcc ctgggcgacg tcttctccca
     1861 gaacaagagc atcaagggaa ccggcgctct cctgtatacg gataccgagg ggaccaggac
     1921 tatccgcctc cacctggacc gcaccaacta cacgcagcta tccgcggtgt tcccgctgct
     1981 cgcggacccc atgatcgcca gcatgggacc gcaggtgaac gacaagatca gcgactccga
     2041 gtacctggag atgatcaagt tcacgttggg tgacgaggcg cccggcctgg tccagaagtc
     2101 cttcgtgacc atcaccatca agccggaagg gcagatcctc tcccagaccg gcggcacgct
     2161 gtccggcggc gcggtagtgt tcaagatccc gctgctgcgc ctgctcgtcc tggacaagcc
     2221 gttggactac accgtgagct acaagtagcg gtcgggaggc gtcgggcgca tgatcgtcat
     2281 cga
//
LOCUS       DASRZS010000234         3064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2056587, whole genome shotgun sequence.
ACCESSION   DASRZS010000234 DASRZS010000000
VERSION     DASRZS010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2056587"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VFH83_05885"
     CDS             complement(<1..732)
                     /locus_tag="VFH83_05885"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET6485928.1"
                     /translation="MDRVRIGLVGFGTVGESFYRLLATNASGFAHGLGAEVEVPYVGV
                     RDASRPRNVNAPTKVIAGWKPILEDPSIPIVVELAGGVETTLPLVREALSRGKHVITA
                     NKALLSAHGKELFELAADRGVELRFEAAVCGGIPIVKVLRESLLGNRIRSIVGIVNGT
                     TNYILTRMTEDKLSFADALSDAQKRGFAEADPTLDVSGRDAAQKIGLLAGLAFGCWVP
                     PEDVLCEGLASVSPKDIEFCKSSGFT"
     gene            complement(720..>3064)
                     /gene="dnaE"
                     /locus_tag="VFH83_05890"
     CDS             complement(720..>3064)
                     /gene="dnaE"
                     /locus_tag="VFH83_05890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET6485929.1"
                     /translation="RGSGAGSLVAYSLRITDIDPLKYGLLFERFLNPERVSLPDFDID
                     FCHRRRDEVIAYVTKKYGEDKVGQVITFGTLGARAVIRDVARTLDLPYDEADAIAKLV
                     PEGPKVDLTSALKESQRLVEVSARGPQYKELMDISLKLEGLHRHPSTHAAGIVIGRKP
                     LTTYVPLYRDPKTEIVSTQYTMDFLEECGLIKMDFLGLKTLTVIEDTLDLIRKRGIPI
                     ELKEIPEDDPATYRMFCEGKSTGAFQFESSGMQGVLRKAKPGRIEDLIALNALYRPGA
                     MEHIDSFVESKSGRKRIEYPLPALEPVLKETYGVPVYQEQVMQIAQVVAGFTLGQADV
                     LRRAMGKKKPEEALKMRPQFISGAKEKGYDEKTAVRIFDLLFAFTGYGFNKSHSAAYA
                     IPAYHTIYLKANYPAEFIAANCTNDMHDTDRLAQLIHEAREMGITVLPPDVNSSLRDF
                     TVEDGKIVFGLLGIKNVGAGSVDAIIAERNANGRFKSFVDFFDRVDAREVNHKAAESL
                     IISGAFDGFHETRATLLHNLQRVMDLSAHGQEARRYGQASLFGGATEEPAARVELEKK
                     PELPTAQLLEEERQNLGFFFSGHPLDPFREIIQRSVTVDLSAKEGLSNDRSCTLVGML
                     HDVREIRTRNNRTMAFAQLEDYRGSIELILFSDIYERHRELIASDAIVGVQGKIDTTR
                     GDPKVKVDEVMDPRSIPQKPVQAVHVRLRDEVGTEESLHEMREYLLERRGDCALYLHV
                     GGDGSPETVVQASSQIRVAGTEEVLSDLRGYPQVADAWTE"
BASE COUNT          483 a         1037 c          984 g          560 t
ORIGIN      
        1 ggtgaatccg ctggacttgc agaactcgat gtccttcggg gacacgctgg ccagcccctc
       61 gcagagcacg tcctccggcg ggacccagca gccgaaggcc agacccgcca ggagcccgat
      121 cttctgggcc gcgtcccgcc cgctcacgtc cagcgtgggg tccgcctccg cgaagccccg
      181 cttctgggcg tccgaaagcg catccgcgaa ggacagcttg tcctcggtca tgcgggtcag
      241 gatgtagttc gtggtcccgt tgacgatgcc cacgatgctt cggatccggt tccccagcag
      301 gctctcccga aggaccttga cgatcgggat cccgccgcac accgctgctt cgaatcggag
      361 ctccacccct cgatccgcgg cgagctcgaa gagctccttg ccgtgggcgg acagcagcgc
      421 cttgttggcc gtgatgacgt gcttgccgcg ggacagggct tcccggacca ggggcagcgt
      481 ggtctccacg ccgccggcca gctccaccac aatggggatg gagggatcct ccaggatcgg
      541 cttccagccg gcgatcacct tcgtgggggc gttgacgttg cgaggcctgg aggcgtcccg
      601 caccccgacg taaggcacct ccacctccgc ccccagaccg tgggcgaacc cggaggcgtt
      661 ggtggccagg agccggtaga agctctcccc gaccgtcccg aacccgacca gaccgatcct
      721 tactcggtcc atgcatccgc cacctgcgga tacccgcgca ggtccgagag cacctcctcc
      781 gtgccggcga cgcggatctg ggacgacgcc tggaccaccg tctccgggct cccgtctccc
      841 cccacgtgca gatacagcgc gcaatctccg cggcgctcca gcagatactc ccgcatctcg
      901 tgcaggctct cctccgtgcc cacctcgtcc cgcagccgca cgtgcaccgc ctggaccggc
      961 ttctgcggga tgctccgcgg atccatgacc tcgtcgactt tcaccttcgg atctcccctc
     1021 gtggtgtcga ttttgccctg aacgccgaca atggcgtccg acgcgatgag ctcgcgatga
     1081 cgctcgtaga tatcggagaa gaggatcagc tcgatgctcc cccggtagtc ctcgagctgg
     1141 gcgaacgcca tggtcctatt gttgcgggtc cgaatctcgc gcacgtcgtg gagcatcccc
     1201 accagcgtgc aggaacgatc gttggaaagc ccttccttgg cggagagatc caccgtgacg
     1261 gaccgctgga tgatctcccg aaagggatcc aggggatgtc cggagaagaa gaaccccagg
     1321 ttctgccgct cctcctccag gagctgggcg gtggggagct caggcttctt ctcaagctcc
     1381 acgcgggccg ccggctcctc cgtggcgccg ccgaagagcg aggcctggcc atagcggcgc
     1441 gcctcctggc cgtgcgcgga gagatccatg acccgctgca ggttgtgcag gagcgtggcg
     1501 cgcgtctcgt ggaagccgtc gaacgctccg ctgatgatca ggctctccgc cgccttgtgg
     1561 ttgacctccc gagcgtccac gcggtcgaag aagtccacga agctcttgaa ccgcccgttg
     1621 gcgttgcgtt ccgcgatgat ggcgtccacc gagcccgcgc ccacgttctt gatccccagg
     1681 agcccgaaga cgatcttccc gtcctccacg gtgaagtccc gcaaggagga gttcacgtcc
     1741 ggcggaagga cggtgatgcc catctcccgc gcctcgtgga tgagctgcgc caggcggtcc
     1801 gtgtcgtgca tgtcgttggt gcagttggcg gcgatgaact cggcgggata gttggccttg
     1861 agatagatcg tgtggtaggc cgggatcgca tacgcggccg aatggctctt gttgaacccg
     1921 tagccggtga aggcgaagag aagatcgaag atgcggaccg ccgtcttctc gtcgtagccc
     1981 ttctccttgg cgccggagat gaactggggc ctcatcttca gcgcctcttc gggcttcttc
     2041 ttccccatgg cccgccgcag gacgtccgcc tggcccaggg tgaacccggc cactacctgg
     2101 gcgatctgca tgacctgctc ctggtagacg ggcaccccgt aggtctcctt gaggaccggc
     2161 tccagcgccg gcaaggggta ctcgatgcgc ttgcggccgg acttgctctc cacgaagctg
     2221 tcgatgtgct ccatggcgcc ggggcggtag agggcgttca gcgcgatcag gtcctcgatc
     2281 cggcccggct tcgcctttcg aagcaccccc tgcatcccgg agctttcgaa ttggaaggcg
     2341 ccggtgctct tgccctcaca gaacatccga taggtggccg gatcgtcctc cgggatctcc
     2401 ttgagctcga tggggatgcc gcgctttcgg atgaggtcca gcgtgtcctc gatgacggtg
     2461 agcgtcttca gacccaggaa gtccatcttg atgagcccgc actcctcgag gaagtccatg
     2521 gtgtactggg tggagacgat ctcggtcttg gggtctcgat agagcgggac gtaggtggtc
     2581 aacggcttgc ggccgatgac gatccccgcg gcatgcgtgg aggggtgtcg gtgcagaccc
     2641 tccaacttca ggctgatgtc catcagctcc ttgtactggg gcccgcgggc ggagacctcc
     2701 accagacgct ggctctcctt gagggcggag gtcaggtcca ccttgggtcc ctcgggcacc
     2761 agcttggcga tggcgtccgc ctcgtcgtag gggaggtcca gcgtccgggc cacgtcccgg
     2821 atgaccgccc gcgcccccag cgtaccgaag gtgatgacct gcccgacctt gtcctcaccg
     2881 tacttcttgg tcacgtaggc gatgacctcg tcccggcgtc tgtggcagaa atcgatgtcg
     2941 aagtccggga gggacacccg ctcggggttg aggaaccgct cgaagagcag accgtatttc
     3001 agggggtcga tgtccgtgat ccgcaggctg tacgccacga gggaaccggc gcccgacccc
     3061 cgcc
//
LOCUS       DASRZS010000235         6183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2057572, whole genome shotgun sequence.
ACCESSION   DASRZS010000235 DASRZS010000000
VERSION     DASRZS010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2057572"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="VFH83_05895"
     CDS             <1..1245
                     /locus_tag="VFH83_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HET6485930.1"
                     /translation="VPVVTLKPAERFQKILGFGGAVTDASCYLFSQLDPAVRDALVAE
                     LFSPTGFGFNVGRITVAQCDFGVVSYSYNDHPGDLAMDHFSIDYDRGYIIPLIRQARE
                     LNGELFLLSSPWSPPGWMKTGGLMTGGWMRGEFLGPFAQYYLRFLQEYAKAGVKIQAL
                     TTQNESETDQLGLMPACLWHPELEMAFLRDHLSPLLRRQGLGDIELWVMDHNYIMWRR
                     AAWMLEDAGLRSVVRGIAWHPYEGEETAMTRVHELHPEVDAHMTEWGGPFDEDMEAVC
                     TRGLRFIDMMRNWSRSIFCWNLALDERGKPHIGPFVQFDQNNGGGLLQIHSASREVRR
                     SSMLYALGHFSKFVKRGAIRIGSDCDVGGIRQVAFINPDGEHVVVLINEGAATEVSLV
                     VGDRFTLVPLTPRSMSTVLFRL"
     gene            complement(1316..2737)
                     /locus_tag="VFH83_05900"
     CDS             complement(1316..2737)
                     /locus_tag="VFH83_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6485931.1"
                     /translation="MGDESRIIPMRRYVLLAVALLVAGACFRAGAQDWPTFGWDVSRS
                     SAPPGSVGLTPGQIGSLKRQQVTLDGTVDSSAIYLRGVSIKGAPHDAFFVTTTYGKTI
                     AIDADTGTVLWEYTPDSYNSLAGSYRITNATPVADDDRTYLYAAAPDGVVRKLAVADG
                     SVVWSTAVTRLPQREKIASPLSFYKGRVLVATDGYIGDRPPYQGHVAILDASNGALLH
                     VWNSLSSDRHELLDPSSVPESDSGIWGRAGVVIDPATGDLYVATGNGTWDGKVNWGDA
                     AIELDPDAQRIVGNFTPVNTSDLNVEDLDLGSTSPVLLGGGLVAQGGKDRVIRILDWR
                     AMQGSAPHRGGEASSVATPSGDRLFTAPAVLHAGSTTWLFAADSGATSAWTLSGTTLQ
                     PRWRVEQGGTSPVVADGMLFVYDPHGGLYVYEATAGRLLSKLSCDLGHWNSPIVGDGR
                     IALPEGNANRHSTNGVLDIWRLP"
     gene            2855..3937
                     /locus_tag="VFH83_05905"
     CDS             2855..3937
                     /locus_tag="VFH83_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HET6485932.1"
                     /translation="MDFLVGFFAPWIIYAVIFVLHVALPARRVAGYVKDASTDRPFAY
                     RLNGPVVLAVTVGLGAIVGGTGLLPWDWLYLHRWAGLAGSCAAGLIFSVIVVLNAPAS
                     GRGFLADFYLGRWENRQFLRGRVDAKMLLYLVGAVMLALNLLSFSAHHLMRYGAAASP
                     GVVVYAALFLWFVVDYLVFEQVHLYTYDLFAERVGFKLGWGCLTFYPYFYAVGLWFTA
                     DLPDPGTPSLVIVICAAVFFLGWTLSRGANMQKFLFKTNPEGALLGVLKPRAIRDGER
                     ALLCSGFWGVARHINYLGEILMATGLTLVLGWPGHLLAWLYPLYYVGLLLPRERSDER
                     RCARKYGELWAEYVRRVPRRIVPGIY"
     gene            complement(3941..4801)
                     /locus_tag="VFH83_05910"
     CDS             complement(3941..4801)
                     /locus_tag="VFH83_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6485933.1"
                     /translation="MQKTVLITGATSGIGLAGAEALARRGWRVLVHGRSDPRVAQALE
                     KLKARVPTGAFEGVSADLGSLSSVAGLAEQVKARTTSLDALWNNAGAVASARATTADG
                     LELQMQVNYLAPYALSLLLLPLLRAAPQGRIVITSSMAHLFAPRRITDWLLTEPGRYR
                     PMAVYGQSKLATILFTQELARRLSNGTVTVNAYHPGFVRSEFGVSRDGGKGASSFSFA
                     HFLAITPEKGADTGVYLVDDPAASRSTGRYWAKRRTRNPSRQATPQAAARLWEESESA
                     VKKVLGALPV"
     gene            5037..>6183
                     /locus_tag="VFH83_05915"
     CDS             5037..>6183
                     /locus_tag="VFH83_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6485934.1"
                     /translation="MIRGARKGIGLLIVLVLLVAGATVSFAAGGKEAGGAKGTVTYWD
                     SVNVADPNDPNVVWQQGNMDAFKAKYPNINVERTIVSNGDEYLNKITTAMAAGNAPDI
                     FQTWLSGRLEPFVTAGRVIPLDDIVNGDSDLKDTITPAFLDTGTFNGHVYALATTLTA
                     EVVFYNKAIFSQYGLSVPATFGDLMKIVSTLKANGIVPFAMGNKDPWVGSIPFMAIFD
                     RVGGQQIYKDIMLAHTSKWNDPAFVQAAKYLLQLRDAGAYPDNFNAMDYNEAVQLFKD
                     GKAGMWFNGTWELASLLKAIPDKDLGFFNFPDIPGGKGTAKSFLLNKDEGYAISSNAK
                     NKDGAVLLLKLMFSKDRQKIRAEAGTLVTTRNLGIDESKLPAITVALL"
BASE COUNT         1032 a         2034 c         2002 g         1115 t
ORIGIN      
        1 gtgccggtcg tgactctcaa gccggcggaa cgcttccaga agatcctcgg tttcggcggc
       61 gctgtcaccg atgcatcctg ctacctcttc agtcagctcg atcccgcggt gcgggacgcg
      121 ctggtggcgg agctcttctc ccccacggga ttcggcttca acgtgggccg gatcacggtg
      181 gcgcagtgcg acttcggcgt ggtgagctac tcgtacaacg atcatccggg cgacctcgcc
      241 atggaccact tctccatcga ctatgaccgc gggtacatca tccccctcat ccggcaagcg
      301 cgcgagctga acggcgagct gttcctcctc tcctcccctt ggagcccgcc gggttggatg
      361 aagaccgggg gcttgatgac cggcggctgg atgcgcggcg agttcctcgg tccgtttgcc
      421 cagtattacc tgcgcttcct gcaggagtac gccaaggccg gggtcaagat ccaagccttg
      481 accactcaga acgagagtga gacggaccag ctcggcctga tgcccgcctg cctgtggcac
      541 ccggagctgg agatggcgtt cctccgcgat cacctgtctc cgctgcttcg gcgccaggga
      601 ctgggggata tcgagctctg ggtgatggac cacaactaca tcatgtggcg gcgagcggcg
      661 tggatgctcg aggacgcggg cctgcgctcc gtggttcgcg gcatcgcgtg gcacccctac
      721 gagggggaag aaaccgcgat gacccgggtc catgagcttc accccgaagt ggacgcgcac
      781 atgaccgagt ggggcggccc gttcgacgag gacatggagg cggtctgcac ccgcggcctg
      841 cggttcatcg acatgatgcg caactggagc cggagcatct tctgctggaa cctggccctc
      901 gacgagcgcg gcaagccgca catcgggccc ttcgtgcaat tcgatcagaa caacggcggc
      961 gggctgctcc agatccattc ggcgagccgg gaggtcaggc gcagctcgat gctgtacgcc
     1021 cttggccact tcagcaagtt cgtaaagcgg ggtgccatcc ggatcggatc cgactgcgat
     1081 gtcggtggta tccgccaggt ggccttcatc aacccggatg gcgagcacgt ggtcgtgctg
     1141 atcaacgagg gggcggcgac cgaggtcagc ctggtggtcg gtgaccgttt cacgctggtc
     1201 cccctgaccc ctcggtccat gagcaccgtg ctgttcaggc tctagccgtt gacaagccgg
     1261 cgcgatagcg catggtgggc gtagcggccc cgaagcgcgt cccccgaagc gcgccctacg
     1321 gcagccgcca gatgtccagg actccgttgg tcgagtgacg gttggcgttg ccctccggca
     1381 gcgcgattct cccatccccg acgatggggc tgttccagtg acccaggtcg cacgatagct
     1441 tcgatagcag ccgcccagcg gtagcctcgt agacatacag cccgccgtgg gggtcgtaga
     1501 cgaagagcat gccgtcggcc accacggggc tggtcccccc ctgctccacc ctccatcggg
     1561 gctggagcgt agttccgctc agagtccacg cggaggttgc gccgctgtcg gcggcaaaga
     1621 gccaggtagt gctgcccgca tgcagcaccg cgggggccgt gaagaggcgg tccccggacg
     1681 gcgtcgcgac tgagctggcc tctccgccgc gatgcggagc gctcccctgc atggcgcgcc
     1741 agtcgaggat tcggatgacg cgatccttcc cgccctgcgc taccagcccg ccgcccagca
     1801 ggaccggcga ggtggagccc aggtcgaggt cctccacgtt gagatccgac gtgttgacgg
     1861 gcgtgaagtt ccccacgatc ctctgggcat ccgggtccag ctcgatcgcg gcatcacccc
     1921 aattgacttt gccgtcccag gtgccattgc cggtcgctac gtagaggtca ccggtcgctg
     1981 gatcgatgac cactcccgct cgtccccaga tcccggagtc gctctcggga accgaggacg
     2041 gatccagcag ctcgtgccgg tcgctggaga gcgagttcca cacgtgcaga agcgcgccat
     2101 tcgaagcgtc cagaatggcc acgtgcccct ggtacggcgg gcggtccccg atgtagccgt
     2161 ccgtcgccac gagcacgcgc cctttataga aggaaagcgg agaggctatc ttctcacgct
     2221 gggggagcct ggtgaccgcg gtggaccaga ccacgctccc gtccgccacg gccagcttcc
     2281 gcactactcc gtccggcgcg gcggcataga ggtaggtgcg gtcatcgtcg gcgaccggtg
     2341 tggcgttcgt gatccgatag ctgccggcca ggctgttgta gctgtccggc gtgtactccc
     2401 agagcacggt cccggtgtcc gcgtctatgg cgatggtctt cccgtacgtc gtggtgacga
     2461 agaaggcatc atggggtgct cccttgatgc tgactcctcg cagatagatt gccgagctgt
     2521 ccacggttcc gtcgagtgtc acctgttgcc gtttcagaga gccgatctgg ccaggggtca
     2581 gccccaccga acccggaggc gcgctggagc ggctgacgtc ccacccgaac gttggccagt
     2641 cctgcgctcc ggccctgaag caagcgccgg caaccaggag cgccaccgca agaagcacgt
     2701 accgcctcat cgggatgatc ctgctttcat cgcccatggc gcgaaagtac gaagcccgcc
     2761 gtcatggggc aatgcgcgcc gttgacggac gtccgctacg gcagtaggct gtctatgtac
     2821 ttcaaatccc ctgaggaccg gagaggacat cacgatggac ttcctggttg ggttcttcgc
     2881 gccctggatc atctacgcgg tcatcttcgt tctgcacgtc gcgctgcccg cgcggcgcgt
     2941 cgccggatac gtgaaggacg cgagcacgga tcggccgttt gcgtaccggt tgaacggccc
     3001 cgtggtgctc gcggtgacgg tcgggctggg ggcaatcgtc ggagggaccg gcctcctgcc
     3061 gtgggactgg ctgtacctgc atcgctgggc cggcctcgcc ggctcctgcg ccgctgggtt
     3121 gatcttcagc gtcatcgtgg tcctgaacgc ccctgcctcg ggtcgcggtt tcctggcgga
     3181 cttctatctc ggccgatggg agaatcgcca gttcctacga ggccgcgtgg acgccaagat
     3241 gttgctgtac ctcgtgggcg cggtcatgct cgctctgaac ctgctgtcat tcagcgcgca
     3301 tcatctgatg aggtacgggg cggcggcgtc tccaggagtg gtggtgtacg ccgcgctctt
     3361 cctgtggttc gtggtggatt acctcgtctt cgagcaggtg cacctctata cctatgacct
     3421 cttcgcggaa cgggtgggct tcaagctggg ttggggctgc ctcaccttct acccgtactt
     3481 ctacgcggtg ggtctatggt tcacggcgga ccttccggat ccggggacgc cctccctggt
     3541 catcgtgatc tgcgccgcgg tcttcttcct gggatggacc ctctcccgag gggccaacat
     3601 gcagaagttc ctcttcaaga ccaaccctga aggcgctctt ctgggcgtcc tcaagccgcg
     3661 cgcgatccga gatggtgagc gcgccctcct gtgcagcggg ttctggggcg ttgcccggca
     3721 catcaactac ctgggtgaga tcctgatggc cacggggctg acgctggtcc tcggctggcc
     3781 gggacacctt ctggcgtggc tctaccccct ctactatgtg gggctgcttc tgccgcggga
     3841 gcgcagcgac gagcgccgct gcgcccgcaa gtacggcgag ctgtgggccg agtacgtgcg
     3901 gcgggtccct cggaggatcg taccggggat ctactgaacg ctagaccggc agcgcgccca
     3961 ggaccttctt gacggcgctc tcggattcct cccagagtcg agcggccgcc tggggtgtcg
     4021 cctgccggga cgggtttcga gtcctccgct tcgcccagta gcgacccgtc gatcgggacg
     4081 cagctggatc gtccaccagg tacacgccgg tatcggcgcc cttctccggg gtgatcgcca
     4141 gaaagtgggc gaaggagaac gagcttgccc ctttgccgcc atcacggctc acgccaaact
     4201 cagagcgaac gaaccccggg tggtaggcgt tcaccgtcac ggtgccgttc gagagtcggc
     4261 gagccagttc ctgggtgaaa aggatggtgg ccaacttcga ctggccgtag accgccattg
     4321 gccgataccg cccgggttcg gtcaggagcc aatccgtgat gcgccgcggc gcgaacaggt
     4381 gggccatcga gctggtgatg acgatccgcc cttgcggcgc ggcccgaaga agcggaagga
     4441 gcagcaggct cagcgcgtac ggcgccaggt agttcacctg catctggagc tccagaccgt
     4501 cagcggtcgt tgcgcgggca gaggccacgg cgccggcgtt gttccacagg gcgtcgaggc
     4561 tcgtggtccg cgccttcacc tgctccgcca gtccggcaac cgacgacagg gaaccgagat
     4621 cggccgacac tccctcgaac gcgcccgtgg ggaccctggc cttcagcttc tcgagcgcct
     4681 gagcgacccg cgggtccgat ctcccgtgga cgaggaccct ccagcctcta cgcgccagcg
     4741 cctccgcgcc cgccaggcca atgccggatg tggcgcccgt gatcagaacc gtcttctgca
     4801 ttgcactctc ctatggcagc accaaggtac gaaagccgtg gccaccggac aacgtgccgg
     4861 cgccccgctc gggcagactg acggaccgcg cgcccctatc cggcatcacc ggtgcgatgc
     4921 tatctttggt gcggcacagc cgcatgagct caggcatccg aatccgtcgg attcggctct
     4981 cagtccggga acgtcgctcg agaggcgggt tcccactcat cgacaagggg gtttgtatga
     5041 tccgaggtgc aaggaagggc atcgggctct tgatcgtcct ggtgctgctg gtcgcgggag
     5101 ccacggtctc cttcgcagca ggcggcaagg aggccggcgg cgccaagggg accgtgacgt
     5161 actgggactc ggtgaacgtc gccgacccga acgatccaaa cgtggtctgg cagcagggga
     5221 acatggacgc gttcaaggcg aagtatccca acatcaacgt ggagcgcacc atcgtctcca
     5281 acggagacga gtacctgaac aagatcacga ccgccatggc cgccggcaac gcgccggata
     5341 tcttccagac ctggctgtcc gggagattgg agccgttcgt cacggccgga cgggtcatcc
     5401 cgctggatga tatcgtcaac ggcgattccg acctgaagga caccatcacc ccggcgttcc
     5461 tggataccgg caccttcaac ggtcacgtct acgccctggc caccacgctg accgcggagg
     5521 tcgtcttcta caacaaggcc atcttctccc agtacgggct ctcggtgccg gccacgttcg
     5581 gcgatctgat gaagatcgtt tccaccctga aggccaacgg catcgttccc ttcgccatgg
     5641 gcaacaagga tccgtgggtg gggtccatcc ccttcatggc gatcttcgac cgcgtgggcg
     5701 ggcagcagat ctacaaggac atcatgctgg cccacacctc caagtggaac gatccggcat
     5761 tcgtgcaagc cgcgaaatac ctgctgcagc tccgcgacgc cggcgcctat ccggacaact
     5821 tcaatgccat ggactacaac gaggccgttc agttgttcaa ggatggcaag gccggcatgt
     5881 ggttcaacgg gacctgggag ctggccagcc tcctgaaggc cattccggac aaagacctcg
     5941 ggttcttcaa cttccccgac atcccgggcg gcaagggaac ggcaaagagc tttctcctga
     6001 acaaggatga gggatacgcc atctcctcca acgccaagaa caaggatggg gccgtcctgc
     6061 tcctgaagct catgttctcg aaggaccggc agaagattcg cgcggaggcc ggcacgctgg
     6121 ttaccacgcg caacctgggc atcgacgaaa gcaagctccc ggccatcacc gtggccctcc
     6181 tca
//
LOCUS       DASRZS010000236         2366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4367441, whole genome shotgun sequence.
ACCESSION   DASRZS010000236 DASRZS010000000
VERSION     DASRZS010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4367441"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /gene="ilvB"
                     /locus_tag="VFH83_05920"
     CDS             <1..1671
                     /gene="ilvB"
                     /locus_tag="VFH83_05920"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HET6485935.1"
                     /translation="EIVFGFPGGVVIPVFDALFGERGLRLVLARHEQGAVHAADGYAR
                     STGKVGVCLVTSGPGATNTVTGLATANFDSVPLVVISGQVTRNMIGNDAFQEADIVGI
                     SRSVTKHNYLVGDRAILGRILREAFFIARSGRPGPVLVDIPKDVLMESSSEPLPETVS
                     IRGYNPVYRGHPLQIRKAATALAQAHKPLFYIGGGLNISGAAEAFRALVQKVDVPTVA
                     SLMGISVLPSDHPRFLGMIGMHGTYAANHAVQNCDLLFAIGTRFDDRATGDIAKFAPH
                     ASVVHVDIDPSSISRNVAVEIPIVGDARLVLEDLLPQAQAPRIEEWIRQTDDWKRSHP
                     LTEVEGKSRLSPTLVLRRIAEEFPEAIITTEVGQNQMWTALYYSFHRPRSFLTSGGLG
                     TMGYGFPAAIGAQLGNPERRVIDIAGDGSIQMNIQELATAITERVPVIVAILNNGYLG
                     MVRQWQEVFYERRYSGVCLERTGECPPDCAAPGDGCPPYTPDFVKLAQAYGAEGIRVS
                     TPAQVDEALRRAKAITDRPIFLDFQIEREANVWPMVPSGAGIHEMLNA"
     gene            1681..2217
                     /gene="ilvN"
                     /locus_tag="VFH83_05925"
     CDS             1681..2217
                     /gene="ilvN"
                     /locus_tag="VFH83_05925"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET6485936.1"
                     /translation="MGTMTHIISLLVENHQGVLARISGLFSGRGYNLESLTVGVTTDP
                     SVSRITLACGGDDAVVEQIKKQLNRLIDVIKVTELTGVPSIHRELMLIKVSAKGRQRG
                     EIFQVADVFKAQVMDVGHDTMALELTGAPEKIEDFVALLLPYGVVEIARSGVVSMERS
                     RKVPVPAGTSRRDEKEEE"
     gene            2214..>2366
                     /locus_tag="VFH83_05930"
     CDS             2214..>2366
                     /locus_tag="VFH83_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485937.1"
                     /translation="MTVERIMIFDTTLRDGEQSPGASMSVEQKLEVALQLARLGVDAI
                     EAGFPIS"
BASE COUNT          414 a          762 c          806 g          384 t
ORIGIN      
        1 gagatcgtgt tcggcttccc gggtggggtc gtcataccgg tgttcgacgc cctcttcggc
       61 gagcgtggcc ttcggctggt gctggcccgt cacgagcagg gagcggtcca cgccgccgac
      121 ggctacgccc gatccacggg caaggtgggc gtgtgcctgg tgaccagcgg tcccggcgcc
      181 accaacacgg tcaccggtct ggccacggcc aacttcgact cggtccccct ggtggtgatc
      241 tccggccagg tcacccgcaa catgatcggc aacgacgcgt tccaggaggc cgacatcgtc
      301 ggcatctcac gctccgtgac caagcacaac tacctggtgg gagaccgggc catcctggga
      361 cgcatcctga gagaggcgtt cttcatcgcc aggagcggga gacccggccc ggtcctggtg
      421 gacatcccca aggacgtgct catggagtcc tcctcggagc cgcttcccga gacggtctcc
      481 atccggggct acaacccggt gtaccgcggg catcccctcc agatccgaaa ggccgccacg
      541 gcgctggcgc aggcgcacaa acccctgttc tacatcggcg gcgggctcaa catctcgggc
      601 gccgcggagg cgttccgcgc cctggtccag aaggtcgacg tgccgaccgt ggcctccctg
      661 atgggcatca gcgtgctccc cagcgaccat ccgcggttcc tcggtatgat cgggatgcac
      721 gggacgtacg cggccaacca tgccgtgcag aactgtgacc tcctctttgc catcggcacg
      781 cgattcgacg atcgagccac cggggatatc gccaagtttg cgccgcacgc ctcggtggtc
      841 cacgtggata tcgatccctc ctccatctcc cgaaacgtgg cggtggagat cccgatcgtc
      901 ggggacgccc gcctggtgct ggaagacctt ctcccgcagg cgcaggcgcc gcgcatcgaa
      961 gagtggatcc gacagacgga cgactggaag cggagccatc ccctgacgga ggtggagggc
     1021 aagagccgcc tgtccccgac cctggtgctg aggaggatcg cggaggagtt ccccgaggcg
     1081 atcatcacga ccgaggtcgg gcagaaccag atgtggacgg cgctgtacta ctccttccac
     1141 cggccgcggt ctttcctgac ctccggggga ttgggaacca tggggtacgg attcccggcg
     1201 gccatcgggg cgcagctcgg gaatcccgag cgccgcgtca tcgacatcgc cggagacggc
     1261 tccatccaga tgaacatcca ggagcttgcg accgccatca ccgagcgggt gccggtgatc
     1321 gtggccatcc tcaacaacgg ctacctgggc atggtccgcc agtggcagga agtgttttac
     1381 gagaggcgct actccggcgt ctgcctggag cgcaccgggg agtgcccgcc ggactgcgcc
     1441 gctccgggag acggctgccc cccgtacacg ccggatttcg tgaagctggc gcaggcatac
     1501 ggcgcggaag gcatccgggt gagcacgccc gcccaggtgg acgaggcgct ccgacgggcc
     1561 aaggcgatca cggaccggcc catcttcctg gatttccaga tcgagcggga ggccaacgtg
     1621 tggcccatgg tgccctccgg cgccggaatc cacgagatgt tgaatgcgtg aggagggtgc
     1681 atgggaacca tgacgcacat catctccctc ctggtggaga accatcaggg ggtgctggcg
     1741 cggatctcag gcctcttctc gggccgtggg tacaacctgg agagcctgac cgtcggcgtc
     1801 accacggacc cctcggtgtc ccggatcacg ttggcctgcg gcggcgacga cgccgtggtg
     1861 gagcagatca agaagcagct caaccggttg atcgacgtga tcaaggtgac ggagctcacg
     1921 ggagtgcctt ccatccatcg ggagctgatg ctgatcaagg tgagcgccaa gggccgtcag
     1981 cggggagaga tcttccaggt cgccgacgtg ttcaaggcgc aggtaatgga cgtgggccac
     2041 gacaccatgg cgctcgagct gaccggtgcg ccggagaaga tcgaggattt cgtggcgctt
     2101 ctcctcccct acggggtcgt ggagattgcc cgaagcgggg tggtctcgat ggagaggagt
     2161 cggaaggtcc ccgtgccggc cgggacgtcc cggcgagacg agaaagagga ggaatgaccg
     2221 tggagcggat catgatcttc gataccacgc tgagggacgg ggaacagtcc cccggcgctt
     2281 ccatgagcgt agagcagaag ctcgaagtag ccctacagct tgcccggttg ggggtggatg
     2341 ccatcgaggc cgggtttccc atctcc
//
LOCUS       DASRZS010000237         6274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1290529, whole genome shotgun sequence.
ACCESSION   DASRZS010000237 DASRZS010000000
VERSION     DASRZS010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290529"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="VFH83_05935"
     CDS             complement(<1..1057)
                     /locus_tag="VFH83_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET6485938.1"
                     /translation="MAKLNGKVLIAQGGGPTAVINQSLVGAVLESRKFPEVTRVYGAT
                     NGVRGIVNEDFLDLSQETTHNLEQVAMTPSSGLLSTRDKPDAAYCKEIFKVCQAHDVR
                     YFFYIGGNDSADTVHIVNQNAETAGYDMRCIHIPKTVDNDLVINDHTPGFGSAARFVA
                     QAFMGFNLDNRALPGVFIGIVMGRNAGFLTAAATLGRKYPDDGPHYCYVPERPFSQEQ
                     FLTDVKSAMDRFGRCVVSVSEGIQDGDGKPFMLKLTGRTEADAHGNVSMSGSGALGDM
                     LSDLVKEKLKIKRVRADTLGYMQRSFMGVVSDVDAHEAREVGEKAAQFAIWHDQDGSI
                     TIQRTGNYSVDYRLVALE"
     gene            complement(1305..1607)
                     /locus_tag="VFH83_05940"
     CDS             complement(1305..1607)
                     /locus_tag="VFH83_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485939.1"
                     /translation="MWQRTIRPTFGQLARQLQYLSTEGLAAVLSRRRHDERTGAAATR
                     LGLLSSEQVDRILRIQRARHRPIGRYFVEKQLLPVTELTRALRDLRRHNERCGTAA"
     gene            2516..2776
                     /locus_tag="VFH83_05945"
                     /pseudo
     CDS             2516..2776
                     /locus_tag="VFH83_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
     gene            complement(2791..3540)
                     /locus_tag="VFH83_05950"
     CDS             complement(2791..3540)
                     /locus_tag="VFH83_05950"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6485940.1"
                     /translation="MEGGDPASLKLYSNLASWFHLVSSPADYGEEAEFYTRTLRGAVS
                     PAPRTVLELGSGGGNNALHMKAHFQMTLSDLSAEMLDLSRTINPECEHIVGDMRTLRL
                     GKRFDAVFIHDAICYMTDEASLKSAIETAVIHCREGGAVLLAPDEVKETFRSTTYQGG
                     HDEGDHGLRFLEWTWDPDPADTTYVADWAYLLRDPSGSVHVEHERHTLGLFDRASWLR
                     VMRELGLKASSLRFDRTGSAPGPTEAFLGLR"
     gene            complement(3544..4398)
                     /locus_tag="VFH83_05955"
     CDS             complement(3544..4398)
                     /locus_tag="VFH83_05955"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6485941.1"
                     /translation="MERRALGKTGEKLSIVGFGGIIVMNEQPADAARVVSQAVDRGIN
                     YFDVAPSYGNAEERLGPALKPYRRKSFLACKTGKRTRVESEAELQNSLKLLQTDHLDL
                     YQHHSVTTMQDVETIMGPGGAMETFVKAKEKGLVRFLGFSAHTEEAALAMLDRFPFDS
                     ILFPFSWVPWLAQDFGRRVMDAAVKKDVARLALKTLAKRKWADGEEKTWPKCWYKPVD
                     TYEEAVSATRFTLSKPITAAVSPGHEQFLWWLCDAAEHLRPLSERDEAELSQRAHDLA
                     AIFPQAAV"
     gene            complement(4498..>6274)
                     /locus_tag="VFH83_05960"
     CDS             complement(4498..>6274)
                     /locus_tag="VFH83_05960"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HET6485942.1"
                     /translation="GSAGTDVRIRHGEVLNPDGTLYRTNLRIVPDRPTWGAQQEDHYL
                     CRGSGDECFEPHFTYHGFRYVEVSGLEKAPVPWDIVGRVLHSTSPEVGTLECSSPVLN
                     RVLAAIQWTQRGNMQGVPTDCPQRDERLGWAGDIQVFSWTAMFNRDMAAFFTKWIRDL
                     REGQAADGRLPDFAPHPYDPNARFSGNPGWSDAALLIPWRMYVAYGDREILAEAFEAA
                     SRWVEYSLRSNPDLIWRDQGACTPAYYGDWLNSDTFVDIPGLPRKGGEVPKEVYATAY
                     FAYSASIAGKMAQVLGKTEEANRYTRLASRVRAAFLKEFVASDGKIKGDTQAGYALAL
                     HFDLLPAALRKKALARLVSALEPYRGGLSTGFLSTVPMMLELVRGGHVEEAYRLLLRR
                     EMPSWCYMVEHGGTTVWERWDGFVEGRGFQNPGMNSFNHYAIGSVGEWMWRVIAGINP
                     LEEAPGCSTVRINPIPGGGLTWARASWRSIRGPLAIEWNRSAETFTMDVTIPPNMSAE
                     IVLPGATAALTTESQTPLARAVGVSLKGRRAEGLVLQVGSGAYHFEADLKTAAPARTK
                     AAVARAKRPAAPRRRKASAPPRRKR"
BASE COUNT         1007 a         2089 c         2094 g         1084 t
ORIGIN      
        1 cctccagcgc caccaggcgg tagtcaaccg agtagtttcc cgtgcgctgg atcgtaatgg
       61 agccgtcttg gtcgtgccag atggcgaact gggcggcctt ctccccgacc tcccgcgcct
      121 cgtgcgcgtc cacgtcggag accacgccca tgaacgaccg ctgcatgtag ccgagggtgt
      181 cggcccgcac gcgcttgatc ttcagcttct ccttgacgag gtccgagagc atgtccccca
      241 gcgccccgga gcccgacatc gagacgttgc cgtgcgcatc cgcctccgtg cgtccggtca
      301 gcttcagcat gaacggcttg ccgtccccgt cctggattcc ctcgctcacg gacactacgc
      361 agcggccgaa ccggtccatg gccgatttca cgtccgtcag gaactgctcc tgggagaagg
      421 ggcgctcggg cacgtagcag tagtgaggcc cgtcgtccgg gtacttgcgt cccaacgtgg
      481 cagccgcggt gaggaagccg gcgttgcgtc ccatgacgat cccgatgaac acccctggca
      541 gcgcgcggtt gtccagattg aagcccatga aggcctgcgc cacgaaccgg gccgctgatc
      601 cgaagccggg cgtgtggtcg ttgatgacca ggtcgttgtc aacggtcttg gggatgtgga
      661 tgcagcgcat gtcgtagccg gccgtctcgg cgttctggtt gacgatgtgg accgtgtcgg
      721 cgctgtcgtt gcccccgatg tagaagaagt agcgcacgtc gtgcgcctgg cagaccttga
      781 agatctcctt gcagtacgcc gcgtccggct tgtcccgggt ggacagcaga ccgctggatg
      841 gcgtcatggc cacctgctcc aggttgtggg tggtctcctg ggaaaggtcc aggaagtcct
      901 cgttgacgat gccgcgcacg ccgttggtgg cgccatagac cctggtgacc tctgggaact
      961 tgcgcgattc caggaccgcg cctaccaggg actggttgat gacggcggtg gggccgccgc
     1021 cctgcgcgat caataccttg ccgttcagct tcgccatggg acatggacct cctgagtggg
     1081 ttatgccggc atgatagcgg gctcaggcgg gtgatgcaag gtgagcgggt gctcgaggtg
     1141 cccaggggcg cccaacgtac cgccgaaaag actcgcgctc tgtcttttcg agtgattccg
     1201 gcggcaagag ccgccaccct caccaaaacg ccgatagcgc tgggaaaacc aacgattctt
     1261 gcgttttcgt gagggtcgag gccccgtgcc tggaaaatcc gtccctaagc cgcggtcccg
     1321 cagcgctcgt tgtgccgccg aaggtcccgc agcgcgcgcg tgagctcggt caccggcagg
     1381 agctgcttct cgacgaaata acggccgatc ggccgatggc gcgcccgctg gatgcgcagg
     1441 atccggtcca cctgctccga gctcaggagc cccagccgcg tagcggccgc gcccgtgcgc
     1501 tcgtcgtggc gccggcggct caagaccgcg gcgaggccct ccgtggacag gtactgtagc
     1561 tggcgagcca gctgtccgaa ggtcgggcgg atggtccgct gccacaccag ggaatggatc
     1621 aggtcctgcc acgagatgcg gccacagtag aagaggtact caccaaggcg aagggagtgc
     1681 ggtggcagag agccacggta gaatagagcg gcgtcgcgac ccggggcctc ggctcgcgaa
     1741 gcctcggcgc gcgaagcttc ggctcgcgga gcctgccgcg gtgtctctgc ccggggagcc
     1801 tctgcccgcg gagcctgccg aggattggct gccggacgcg tccgggcggc gggacgtggc
     1861 tggggagctg gacgtggcct tgctgccgcc ctcgcggcgc gcggtctgct ggcggcccgc
     1921 tgcggccggg tgctgagcac gtgtcgcatc accagctcgt acgcttggga cgcctccaca
     1981 aaccgcctgc cgtccgctgg tccggatctt ccgccccgcc gcgccgccgc gtccgggtgg
     2041 acggagatgg ccagccggcg gtacgccctt cggatcaccg acgggtcgac ctcatccagg
     2101 gctccgatcc ctaggaccgc gcatgcgtcg agcagcttgc tctccatatc caccattatc
     2161 ggctcgatgc gccgattccg gcaagcccaa accggccatt ccatccggac cggctctgct
     2221 ccgcaagatc cgggtggcga ggccggagag accgtactac cctgcaagca tgaacacaaa
     2281 gctgacggta tacaccggtg gcgacatcgg catccggtat gggtttcatc caacgctatt
     2341 cggggaggcc atgatcgccg tggcggatca gggcatttgc gctctttggt tcgtggggga
     2401 cggctcccgc gcggaggcga tggcgcgtct gacaaaggag tggagcctcg cggacatcga
     2461 ggcggaccag gaaggcacag cgggaattgc gaagcggatc ttcacacatc tgcctatggg
     2521 agagctcaag gttcacgtgc aggggaccac gttccaggtc aaggtctggg aagctttgct
     2581 ttccctggac ccggggcagg tcaccaccta tgagaccatg gcccagcgcg tgggggatcc
     2641 ctccgccctt cgggccatgg gaagcgcaat cggccgaaac ccggtggcgt tcgtgatccc
     2701 gtgccatcgc gtgatccgca aatccggtga gctcggtggg taccgctggg ggctggaccg
     2761 caaacgcgaa atcctggact gggagtccgc ctaccgcaga cccaggaacg cctccgtggg
     2821 gccgggcgcc gaacccgtgc gatcgaatcg gagggaggat gccttcagcc caagctcgcg
     2881 catgacccgc agccaggagg cgcggtcgaa cagccccagg gtgtgacgct cgtgctcgac
     2941 gtggaccgag ccgctcggat cccgaagaag gtaggcccag tcggccacat aggtggtatc
     3001 cgccgggtcg ggatcccagg tccattccag gaagcgcagg ccatgatccc cttcgtcgtg
     3061 gccgccctgg taggtggtgg accggaaggt ctctttcacc tcgtccgggg ccagaagcac
     3121 ggccccgcct tcccggcaat gaatcaccgc ggtctcaatg gcgctcttca gggatgcctc
     3181 atcggtcatg tagcagatgg cgtcatggat gaacacggcg tcgaaccgct tccccagtct
     3241 gagggtccgc atgtctccga cgatgtgctc gcactccggg ttgatcgtcc ggctcaggtc
     3301 gagcatctcc gcggagaggt cgctcagggt catctggaag tgcgccttca tgtgcagcgc
     3361 gttgttgccg ccgccgcttc ccagctccag gaccgtccgg ggcgccggtg acacggcgcc
     3421 tcggagcgta cgggtgtaga actccgcctc ttccccgtag tctgccgggg aggacacgag
     3481 atgaaaccac gaggcgaggt tggagtaaag cttcagcgag gccgggtcgc cgccctccac
     3541 ggcctagacc gcggcctgag ggaagatggc ggccaggtcg tgcgccctct gggatagctc
     3601 cgcctcgtcg cgctcggaga ggggccgcag atgctcggcc gcgtcgcaca accaccagag
     3661 gaactgctcg tgccccgggc tcaccgcagc ggtgatcggc ttggaaagcg tgaagcgggt
     3721 cgccgagacg gcctcctcat acgtgtccac cggcttgtac cagcacttcg gccaggtctt
     3781 ctcttcaccg tccgcccact tccgcttggc aagggtcttg agcgccagcc gcgccacgtc
     3841 cttcttgacg gccgcgtcca tgacccttcg gccgaagtcc tgggccagcc acggcaccca
     3901 gctgaacggg aagaggatgc tgtcgaacgg gaaccggtcc agcatggcca gggccgcctc
     3961 ctccgtatgg gcggagaacc cgaggaagcg gaccagaccc ttctccttgg ctttcacgaa
     4021 ggtctccatg gcgccaccgg ggcccatgat ggtctccacg tcttgcatgg tggtaaccga
     4081 gtgatgctgg tacaggtcga ggtgatcggt ctgaaggagc ttgagagagt tctgaagctc
     4141 cgcttccgac tccacccggg tgcgcttgcc ggtcttgcag gccaggaagc tctttcggcg
     4201 atagggcttc agagccggcc ccaatcgttc ctcggcgttt ccatacgagg gcgcgacgtc
     4261 gaagtagttg atgccgcggt ccaccgcctg gctcaccacc cgggcagcat ccgcaggctg
     4321 ctcgttcatc acgatgatgc cgccgaaccc cacgatggac agcttttccc cggtcttccc
     4381 gagcgctcgt cgttccatgg tgcctccccg attgacttgg gttcaggata ctgccgcgcg
     4441 ctcccgcccg gcaaccgagc gcggcctctt gccggccctt cccggcgcag cggccggtca
     4501 gcgcttgcgg cgcggaggag cggaggcctt cctgcgcctg ggggcggcgg gcctctttgc
     4561 ccttgctacg gcggctttcg tccgcgccgg cgccgccgtc ttgaggtccg cctcgaaatg
     4621 gtacgcgccg gatcccacct gcaggacgag cccctcggct ctacggccct tcaggctcac
     4681 cccgacggcc cgggccaacg gggtctggct ttcggtggtc agggctgcgg tggcccccgg
     4741 caggacgatc tccgcgctca tgttcggcgg gatcgtgacg tccatcgtga aggtctcggc
     4801 ggaacggttc cactcgatgg ccaacgggcc ccggatggag cgccaggaag cgcgcgccca
     4861 cgtgagccct ccgcctggaa tgggattgat gcgtaccgtc gagcagcccg gcgcctcctc
     4921 cagagggttg atgccggcga tcacccgcca catccactct ccgacggagc cgatggcgta
     4981 gtggttgaac gagttcatgc cggggttctg gaatccgcgg ccttcgacga acccgtccca
     5041 tcgctcccag acggtggtcc caccgtgttc aaccatgtag caccatgaag gcatctcgcg
     5101 cctcaggagc aggcggtagg cctcttccac gtggccgccg cggaccagct ccagcatcat
     5161 gggcacggtg ctcagaaagc cggtagaaag accacctcgg tagggctcga gcgcggacac
     5221 gagccgggcc agggctttct tccgaagagc ggcgggcagg aggtcgaaat gcagtgccag
     5281 ggcgtagccc gcctgcgtat cgcccttgat cttcccgtcc gacgcgacga actctttgag
     5341 aaaggcggcc ctgacgcgtg atgccagcct ggtgtaccga ttggcttcct cggtcttccc
     5401 gaggacctgc gccatcttcc cagcgatcga agcggagtag gcgaagtagg cggtggcata
     5461 cacttccttc gggacttcgc ctccctttcg ggggagaccc gggatgtcca cgaaggtgtc
     5521 cgagttcagc cagtcgccgt agtacgccgg cgtgcaggcg ccctggtctc gccagatcag
     5581 gtccgggttc gatcgaaggg aatactcgac ccaccggctg gccgcctcga aggcctccgc
     5641 caggatctcg cgatccccgt aggccacgta catccgccac gggatgagca gcgcggcgtc
     5701 cgaccatccg gggttgccgc tgaagcgcgc gttggggtca taggggtgtg gggcgaagtc
     5761 cggcagtctc ccgtccgccg cctgaccctc ccgcaggtcg cggatccact tcgtgaagaa
     5821 ggcggccatg tcccggttga acatggcggt ccacgagaag acctggatgt ccccggccca
     5881 ccccagcctc tcgtcccgct gcgggcagtc ggtcgggacg ccctgcatgt tgccgcgctg
     5941 ggtccattgg atggcggcca ggacccggtt gagcacgggg ctggaacact ccagggtccc
     6001 gacctccggc gacgtggagt gcagcacccg acccacgatg tcccagggga ccggggcctt
     6061 ctccagaccg ctcacctcca cgtagcgaaa gccgtggtac gtgaagtggg gctcgaagca
     6121 ctcgtctccc gagccgcggc ataagtagtg gtcctcctgc tgcgcgcccc aggtaggccg
     6181 atccggcacg atgcgcaggt tggtccgata cagcgtgcca tccgggttca gcacctcgcc
     6241 gtgacggatc cgaacgtccg tcccggcgga gccg
//
LOCUS       DASRZS010000238         2613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2829949, whole genome shotgun sequence.
ACCESSION   DASRZS010000238 DASRZS010000000
VERSION     DASRZS010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2829949"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFH83_05965"
     CDS             <1..614
                     /locus_tag="VFH83_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6485943.1"
                     /translation="HTLRRKGAAYWYRVLLILPTVVPGLVTLFIWIIFFHRDPSIGLF
                     NSIGTAIFGKDAIVQWLSDPKVTFWSLVLIGFPWAGGFAMLIYLAGLQTIPLELYDAS
                     MIDGASSFRTFRSLEVPLIRGQLKLFVVLAIIGSLQNFTIPYIMTGGGPRFTTMVPGL
                     LVYRRAFSENLFGYASAIAFVLFLVTMSLSYLNIRFIQSKVEY"
     gene            629..1501
                     /locus_tag="VFH83_05970"
     CDS             629..1501
                     /locus_tag="VFH83_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6485944.1"
                     /translation="MANPPHETAPMVGSPVPPRRRIRLQFLPHAVLLILVVTTFYPLV
                     FTLLASLKSNAQFYSTFWVPTAPFHWENYLKAWKELSRGFLNSVVVSSVSVTGVVLMS
                     GLAAWTFARYRWPGKEVLYYLILGLMMVPAFLTLVPLYWMVRELRLTGSLAAVFLPYL
                     AGGQVFPIFLMRAFFENLPGELFDSARLDGAGEITIFLRIGMPLCVPIVITVAIMDVM
                     GTWNDFFWPLLAVGQNRDLYTVMVRTFNLTGLMPDYGLLTSAYVIISVPLILMFIFGM
                     RYYISGLTSGAVKM"
     gene            1513..>2613
                     /locus_tag="VFH83_05975"
     CDS             1513..>2613
                     /locus_tag="VFH83_05975"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HET6485945.1"
                     /translation="MDATTLRRVEGYARLRPLPPGRVVPGGWLRHYLQVTADAWPLLY
                     ARNRDPEVYGKFWTRNKTSKVTFDENNVTQVLCDYTAYFAEGLLHYAQLLPESALARE
                     ADDWVSRLLSSQDADGYVGAFDPGARWQHWLEVFSQSVVEDAILHRYEGTGDRRLLEA
                     CERMARLQMSAWYRPAADSVAGIWTGHGTVVIRSLLKLYELTGDAGYLGCARDILQKH
                     GKTSEFLTSRDALENVHNAVGTEHVGFPALLYEFTGDPSLLVASVSGWEAESRLHLSV
                     DGTPHGNESMLFKGPLHNCEHCGTVEWIYASNALARMTGEVRFADAVERAIHNAYPAA
                     RSADGLSVAYMHTPNQIAATEWSQPHGWTSPDW"
BASE COUNT          407 a          895 c          804 g          507 t
ORIGIN      
        1 tccacacgct ccgccgcaag ggcgcggcat actggtaccg cgtgcttctg atcctgccga
       61 ccgtggtgcc gggccttgtc acgctcttca tctggatcat cttcttccat cgggacccct
      121 cgatcgggct gttcaacagc atcggcacgg ccatcttcgg caaggacgcc atcgtccagt
      181 ggctgagtga ccccaaggtc accttctggt cgctggtgct catcgggttt ccctgggcgg
      241 gcgggttcgc catgctgatc tacctggcgg ggctccagac catcccgctg gagctctacg
      301 atgcgtcgat gatcgacggc gcctcctcct ttcgcacctt ccggtccctg gaggtgccgc
      361 tcatccgcgg gcagctcaag ctcttcgtgg tcctggcgat catcggcagc ctgcagaact
      421 tcacgattcc ctacatcatg acgggcgggg gtccccggtt caccaccatg gtgcccggcc
      481 tgctggtgta ccgccgcgcg ttttcggaga acctcttcgg atacgcgtcg gccatagcct
      541 tcgtcctctt cctggtcacg atgagcctga gctacctgaa catccggttc atccagtcga
      601 aggtcgaata ctgaaggggt ggatccagat ggcgaaccca ccccacgaaa cggcccccat
      661 ggtcggctcc ccggtccctc cgcgccgcag gatccgtctc cagttccttc ctcacgcggt
      721 gctcctgatc ctcgtggtga ccaccttcta tccgctggtg ttcaccctgc tggcttccct
      781 caagagcaac gcccagttct acagcacctt ctgggtgccg accgccccct tccactggga
      841 gaactacctc aaggcgtgga aggagctctc ccgaggcttt ctcaactccg tcgtggtgtc
      901 ctcggtgagc gtgacggggg tcgtcctcat gtccgggctg gcggcctgga cgttcgcgcg
      961 atatcggtgg ccggggaagg aagtcctgta ctatctcatc ctgggcttga tgatggtacc
     1021 ggccttcctg acccttgtcc cgctgtactg gatggtccgg gagctccggc tgacgggcag
     1081 cctggccgcg gtcttcctgc cttacctggc gggaggccag gtgttcccga tctttctcat
     1141 gcgggccttc ttcgagaacc tgcctgggga gctcttcgac agcgcccgtc tggacggcgc
     1201 gggagagatc accatcttcc ttcggatcgg catgcccctg tgcgtgccca tcgtgatcac
     1261 cgtagccatc atggacgtca tggggacctg gaacgacttc ttctggccgc tcctggccgt
     1321 gggacagaac cgtgacctgt acacggtcat ggtccgaacg ttcaacctga cggggctgat
     1381 gccggactac gggcttctga cctccgctta tgtcatcatc agcgtccccc tgatcctgat
     1441 gttcatcttc ggcatgcggt actacatcag cggcctgacg agcggcgccg tgaagatgtg
     1501 agaggaggaa ccatggacgc cacgaccctg cgacgagttg aaggatatgc ccgtctgcgg
     1561 ccgcttccgc ccggccgcgt cgtgcccggc ggctggcttc ggcactacct ccaggtgacc
     1621 gcggacgcgt ggcccctgct gtacgcgcgc aaccgtgacc ccgaggtcta cggaaagttc
     1681 tggacccgca acaagacctc caaggtcacc ttcgacgaga acaacgtgac ccaggtgctc
     1741 tgcgactaca cggcgtactt cgccgaaggt ctcctccact atgcgcagct tctccctgag
     1801 agcgcgctgg cccgggaagc ggacgactgg gtctcccgtc tcctgtcctc ccaggacgcg
     1861 gacggctacg tgggcgcctt cgatcccggc gcccgctggc agcactggct ggaggtgttc
     1921 tcccagtccg tggtggagga tgccatcctg caccgctacg agggcacggg ggaccggcgc
     1981 ctcctggagg cctgcgagcg gatggcccgg ctccagatga gcgcctggta tcgaccggcc
     2041 gcggacagcg tggccggaat ctggaccggc cacggcacgg tggtcatccg gtccctcctc
     2101 aagctctacg agctcacggg agatgcgggc tacctgggct gcgcccggga catcctccag
     2161 aagcacggaa agacgagcga gttcctcacg tcgagggacg ccctggagaa cgtccacaac
     2221 gcggtgggta ccgagcacgt cggcttcccg gcgctgctct acgagttcac gggggatccg
     2281 tccctcctgg tggcgagcgt ctccggctgg gaggccgagt cccggctgca tctctccgtg
     2341 gacggcacgc cgcacggcaa cgagtccatg ctcttcaagg gtcccctcca caactgcgag
     2401 cactgcggca ccgtggaatg gatctacgcc agcaacgcgc tggcccgaat gaccggcgag
     2461 gttcggttcg cggacgccgt ggagcgcgcc atccacaacg catatcccgc ggcgcgttcg
     2521 gcggacggcc tctccgtggc atacatgcat accccgaacc agatcgcggc gacggagtgg
     2581 agccagcccc atggctggac cagtccggac tgg
//
LOCUS       DASRZS010000239         4168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_135877, whole genome shotgun sequence.
ACCESSION   DASRZS010000239 DASRZS010000000
VERSION     DASRZS010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135877"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2045)
                     /gene="mgtA"
                     /locus_tag="VFH83_05980"
     CDS             complement(<1..2045)
                     /gene="mgtA"
                     /locus_tag="VFH83_05980"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HET6485946.1"
                     /translation="MRRSTAGAVATERFLLDACTASAEECLSRLSTRDTGLTEEEVEE
                     SRRRFGRNEVSVKKTGIFGRIYHRFANPLVIQLLVIAGVSFLMGDIRAGSVVAGMIVI
                     STVLSFVQEERSGRAVEKLQAMVSTTANVLRDGKQSEIPLAEIVPGDIVILDAGSLVP
                     ADLRLIATKDFFVSQSALTGESMPVEKSVAGSSTNGASALEQPNACFQGSNVLSGTAR
                     GVVVNTGERTYFGAITRKLGAQRIHTSFDKGIAGFTWLMIRFMVVMVVVVFLIIGITK
                     HSWLDALLFALAVAVGLTPEMLPMIVTVNLAKGAIAMSRKKAIVKRLASIQNFGAIDI
                     LCTDKTGTLTQDRVVLEKHVDVTGRDSDDVLRYAYMNSYYQTGLRNLLDRSVLSHTEF
                     EVERKCRKVDEIPFDFTRKRMSVVVDYEGDHVLICKGAVEDVFKVCASYQVDDEVFPL
                     IDVIRQDVLEDYEGLSADGYRVLAIAYREFERTKEVFSAADEGELILLGYIAFFDPPK
                     ESSAQALKALKDAGVMVKILTGDNDLVTRKVCKDVGIDVEHLVTGPQLQGLSAEALVT
                     TAQSATVMARLSPTQKEQVIQALQGAGHVVGFLGDGINDAPALKRADVGISVDTAVDV
                     AKESADIVLLEKSLLVLDDGIIEGRKVFGNIVKYIRMGASSNFGNMFSVLGGALFLP"
     gene            complement(2088..3230)
                     /locus_tag="VFH83_05985"
     CDS             complement(2088..3230)
                     /locus_tag="VFH83_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485947.1"
                     /translation="MSITPCRPPWKGRLTARERFNNQMHYRGVDRCFNMEFGYWDENF
                     TLWDIFSKNGIRTNEEADRFFSFDRFEVVSPPFWMHPQFESRVVSETATTRVLINKDG
                     LFAESPRDGHDTIPHFVKASIVTPEDWKRAKAERFRLDDPARKVDVEAIRRLHPANRD
                     YPLGVDCGSMIGRVRDVLTLEGLAYAVYDYPQMVEDMVETSCQLVEAFLDQVLGKIDF
                     DFASGWEDIAFKNGPIVSVGFFKDVIVPRYKRISRKLHAAGIDLWYTDCDGDVRPILP
                     LFLEGGINCLFPFEVNSCTHPRDLLEAYGTDLRIMGGVDKIELGKGPGAIRAYLDTLV
                     PIVTRGGYIPFCDHRCPPNVKPADYLFYLDLKEKMFGLGSALYPEP"
     gene            3421..>4168
                     /locus_tag="VFH83_05990"
     CDS             3421..>4168
                     /locus_tag="VFH83_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6485948.1"
                     /translation="MIAMFLAASASLGFSQQTAPASQQTAAGPAPAATGSEPAVTLDQ
                     AVAAAMVSAPGLTVARVALDTARSQLTEVQATNGLSLAARGGYSHQGDLTGGSAATGA
                     DASAAAAASGSGVRGDTVQGGLDLSGPSTSVGLTAQHSITPAPSDQVSGLSLSASQTV
                     FDGYAGGRASALVQQAQDTYRIAQVAFDAAQKGVTYQVKQAYYTLLTDQETLQVRQVT
                     VQQAQENLAYYQGLLQAGRVTSLEVLLVQVT"
BASE COUNT          709 a         1358 c         1365 g          736 t
ORIGIN      
        1 aaggggagga agagcgcgcc gcccagaacg ctgaacatgt tgccgaagtt ggagctggcg
       61 cccatccgga tgtacttgac gatgttcccg aatactttcc gcccctcgat gatcccatcg
      121 tccagcacca ggaggctctt ctccagaagg acgatgtcgg ccgactcctt ggccacgtcc
      181 accgcggtgt ccacggagat ccccacgtcc gcgcgcttca gggccggcgc gtcgttgatc
      241 ccgtccccca ggaaccccac cacgtgaccc gcgccctgga gcgcctggat gacctgctcc
      301 ttctgggtgg gggagagccg cgccatcacg gtggcggact gtgcggtggt taccagcgcc
      361 tccgcggaga gaccctggag ttggggaccg gtgaccaggt gctccacgtc gatcccgacg
      421 tccttgcaga ccttgcgggt gaccaggtcg ttgtcacccg tgaggatctt gaccatgacg
      481 cctgcgtcct tgagcgcctt cagcgcctgg gcggaggact ctttgggcgg atcgaagaac
      541 gcgatgtagc ccagaaggat cagctcgccc tcatccgcgg cggagaagac ctccttggtg
      601 cgctcgaact cccggtaggc gatggccagc acccggtagc cgtccgcgct caggccctcg
      661 tagtcctcca ggacatcctg gcggatcacg tcgatcaagg ggaagacctc gtcgtccacc
      721 tggtaggacg cgcagacctt gaacacgtcc tccacggcgc ccttgcagat caggacgtgg
      781 tctccctcgt agtccaccac cacggacatg cgcttgcgcg tgaagtcgaa cgggatctcg
      841 tccaccttgc ggcacttgcg ctccacctcg aactccgtgt gggaaagcac ggaccggtcc
      901 aggaggttgc gcagccccgt ctggtagtag ctgttcatgt aggcgtagcg cagcacgtcg
      961 tcgctgtcgc ggcccgtgac atccacgtgc ttctccagca ccacccggtc ctgggtgagg
     1021 gttcccgtct tgtcggtgca gaggatgtcg atggcgccga agttctgaat ggaggccagc
     1081 cgcttgacga tggccttctt gcgcgacatg gcgatggcgc ccttggccag gttcacggtc
     1141 acgatcatgg gaagcatctc gggggtcagg ccgaccgcga ccgccagagc aaagaggagc
     1201 gcgtcgagcc agctatgctt ggtgatcccg atgatgagga agaccaccac caccatgacc
     1261 accatgaagc ggatcatgag ccaggtgaag cccgcgatcc ccttgtcaaa gctggtgtgg
     1321 atgcgctggg cgcccagctt tcgcgtgatg gccccgaagt aggtcctctc tccggtgttg
     1381 accaccaccc cgcgcgcggt cccagagagc acgttgctcc cctggaaaca ggcgttcggc
     1441 tgctccagag cggaggcgcc gttcgtggag gagccggcca cgctcttctc caccggcatc
     1501 gactccccgg tgagcgcgct ctggctgacg aaaaagtcct tcgtggcgat gagacgcagg
     1561 tccgccggca ccagggagcc ggcatccagg atcacgatgt ctccgggtac gatctccgcc
     1621 agggggatct cggactgttt cccgtcccgc aggacgttgg cggtggtgct gaccatggcc
     1681 tggagcttct ccacggcccg accggatcgc tcttcctgga cgaaggagag caccgtgctg
     1741 atgacgatca tgccggccac caccgacccc gcgcggatgt cccccatcag gaaggagacg
     1801 ccggcgatca ccaggagctg gatgaccagg gggttggcga aacgatggta gatccggccg
     1861 aagatgccgg tcttcttgac gctgacctcg ttgcggccga accggcggcg gctctcctcg
     1921 acctcttcct cggtgagacc ggtatcccgc gtggagaggc ggctcaggca ctcctctgcc
     1981 gaggcggtgc aggcgtccag caggaagcgt tccgtggcta cggcgccggc ggttgaccgt
     2041 ctcatccgca aggcctcccg tccgccacga acgcgcgtct catcggtcta cggttccggg
     2101 tacagcgccg agcccagacc gaacatcttc tccttgagat cgaggtagaa gagatagtcc
     2161 gccggcttga cgttgggcgg gcagcggtga tcgcagaacg ggatgtaccc gccccgcgtt
     2221 acgatgggta ccagggtgtc cagatacgcg cggatggccc cgggtccctt tccgagctcg
     2281 atcttgtcca cgccgcccat gatgcgcagg tccgtcccgt acgcctccag gaggtcccgg
     2341 gggtgggtgc aggagttgac ctcgaagggg aagaggcagt tgatcccgcc ctccaggaac
     2401 agcggaagga tgggccgcac atcgccatca cagtcggtgt accagaggtc gatcccggcc
     2461 gcgtggagct tgcggctgat cctcttgtag cggggcacga tgacgtcctt gaagaacccg
     2521 accgacacga tgggtccgtt cttgaacgcg atgtcttccc atccggatgc gaagtcgaaa
     2581 tcgatcttgc cgagcacctg atccaggaac gcttcgacga gctggcagga ggtctccacc
     2641 atgtcctcta ccatctgcgg gtagtcgtac acggcgtagg ccaggccctc cagggtcagg
     2701 acgtcccgga cccgtccgat catggatccg cagtccacgc ccagggggta gtcccggttg
     2761 gcgggatgga gacgacggat ggcctccacg tccaccttcc gagcggggtc gtccaaccgg
     2821 aagcgctccg ccttggccct cttccagtcc tccggcgtga cgatggacgc cttcacgaag
     2881 tgcgggatgg tatcatgacc gtcccgcggc gactcggcga agaggccgtc cttgttgatg
     2941 aggacgcgcg tggtggccgt ctccgagacg acccggctct cgaactgcgg gtgcatccag
     3001 aacggcgggc ttaccacctc gaagcggtcg aagctgaaga accggtccgc ctcttcgttc
     3061 gtgcggatcc cgttcttgct gaagatgtcc cagagcgtga agttctcgtc ccagtagccg
     3121 aactccatgt tgaagcatcg gtccacgccg cggtagtgca tctggttgtt gaaccgctcc
     3181 cgggcggtaa ggcggccctt ccatggcggt ctgcagggag tgatgctcat ggcagcgaaa
     3241 gttagcacaa ggcggagggg cctgtacagg gggaacctcg actcccatgg cgatttcccg
     3301 cgcttaacac gaagttatcc gcctttcatg ggccttctat ggtgggccgg cagcacgcgg
     3361 accatattca ccatggaggg tgaaggtgtg agtgcaaaag gaacgcggag atcgttgctg
     3421 atgatcgcga tgttcctggc cgcgagcgcg agcctgggct tttcccagca gacagcgccc
     3481 gcctcccagc agacggcggc gggtccggcc ccggcggcga caggctcgga gccggccgtg
     3541 acgctggacc aggcggtggc cgcggccatg gtgtccgcgc cgggcctcac cgttgctcgc
     3601 gtggctctgg ataccgcgcg gtcccagctc acggaggtcc aggcgaccaa cggtctctcc
     3661 ctcgccgcgc gcggcggata ctcgcaccag ggggatctca cgggaggatc tgcggcgacc
     3721 ggtgccgacg cctctgcggc ggctgccgca agcgggtccg gtgttcgcgg tgacaccgtc
     3781 cagggcggtc tcgacctctc ggggccatcc acgagcgtgg gcctcacggc ccagcatagc
     3841 atcacgccgg cgccgtccga ccaggtgagc ggcctgagcc tctccgccag tcagacggtt
     3901 ttcgacggat acgcgggcgg tcgcgcctcc gctttggtgc agcaggctca ggatacgtat
     3961 cggatcgcgc aggtggcatt cgacgccgct cagaaaggcg tcacctacca ggtcaagcag
     4021 gcgtactaca cgctgcttac cgaccaggag acgctgcagg tccggcaggt caccgtccag
     4081 caggcgcagg agaaccttgc ctactatcag ggtctcttgc aggccgggcg cgtcacgtcc
     4141 ctggaggtgc tcctggtaca ggtcacgc
//
LOCUS       DASRZS010000240         5116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3983683, whole genome shotgun sequence.
ACCESSION   DASRZS010000240 DASRZS010000000
VERSION     DASRZS010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3983683"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VFH83_05995"
     CDS             complement(<1..601)
                     /locus_tag="VFH83_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6485949.1"
                     /translation="MKRVAGFLALLLAVPAVAALAAGSGEKAAGSSAAQPAPVSVMTY
                     MGHVATDEQYKAIHDYIEKQTGVKFTYTDVKDADSYTTQITAAIAAQQAIDLFTVGNK
                     VSLANYRSSGAIQDITDNVNTYAPNIKKLFNNPPGWSSLPPGEMWKAVTSNGRIWAIP
                     QASGTNVGVVLQVRKDWRVKLGLPPITTFDQFEQFLRAVK"
     gene            complement(823..1689)
                     /locus_tag="VFH83_06000"
     CDS             complement(823..1689)
                     /locus_tag="VFH83_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET6485950.1"
                     /translation="MTRYIAAYDTEMSAASRPGPHVPTCLEACRRIVEVHRAYRMPAT
                     FFIVGRTLEESPSEYRALLDDPLFEVASHSYSHRMLLDHPICGPAADRAQVEREIRQG
                     KAVVEDVFGRPCVGFRPGCGFVEGLRGATVALDLVAEAGYRYVSSVLWGADFSLPAPL
                     AEPWSYASDGHPEINEYPAHGWHENLLKDNNRVFGQEPQRVVLFPTPFAAAVPPHPVR
                     TPQEEIQYNNGYFIDAAIAAHSSYVTLIWHPWSLALSDPAMRVLEMTFQLVRDRGLPA
                     TTFAEMDRSRRA"
     gene            1820..3397
                     /locus_tag="VFH83_06005"
     CDS             1820..3397
                     /locus_tag="VFH83_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6485951.1"
                     /translation="MTRRAGVLVGAIAMIAVLGGAAFAAPVSGGTLKIAVLSLESLEL
                     YKTAANDITDAQSVIFDSLFQIGKDNFQPIPCLASGWTNPDPTTWVFTIRKGVLFQDD
                     NPVFPKGKSREVTADDVVYSINFMMKTSTAWTLGPVTSVKALDRYTVEIKTDSPQPFL
                     VNDPNRLCRVMIVPKEAIDQLGADGFAKYPIGTGPYKFKSFSPDSGLLLVRNPLYRLP
                     VYPDAVEMVVIPDPVAQTLALQSGEVDVIKHVFNLEMVDTLQKDPNLIVTRAPGGSYR
                     GIGFNVTVPPYNVLAVRKGLSQILDIDAAYKAVIGNKGERAYGQVPPWVAFGYDPSLK
                     DLWSYNPADGLKLLMANGFSKVAGDQFLEFNGKPFQIQIKTLAGSQVQALTILATQLR
                     EQGINASVLTEDTAVWADDLVKGNTGMFFDYSFAGTTGFHSLFHGSMIGSANTHFYNN
                     PAVNTLLDQALTKVNIDELSVLWKKAQRLVMQDVVGIPLYFEYSYAAVNRKVNDFVPP
                     WGGLNLVSIENNVWLSR"
     gene            3476..4423
                     /locus_tag="VFH83_06010"
     CDS             3476..4423
                     /locus_tag="VFH83_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485952.1"
                     /translation="MGRYLLRRVLTTIPVLFGIVFIVYAMISFVPGNTISLMLSNESV
                     NVANVEKLKIELGLDKPWYTRFVLSMGKLVRGDLGRSMQSNRPVADMIRERIGNTFRL
                     TVFSCLVALLVSVPLGVLAAARRRSVFDYLATALALLGVSTPSFYLGLLLILLFGLKL
                     HWLPIRGLPSYNATFLGQIRYLILPSVTLGSALTGILTRLTRASMLEALGQDFARTAR
                     AKGQSQAGVLFRHALPNAFLPVLTTIGLQFGSLLGGAVIIETIFSLPGLGLLALNAVK
                     FRDVPLIQGTVLVFAVCFIAVMLVVDILYVFVDPRIRLD"
     gene            4425..>5116
                     /locus_tag="VFH83_06015"
     CDS             4425..>5116
                     /locus_tag="VFH83_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6485953.1"
                     /translation="MARSDRRRVAVPRLLRVFMRHRLGIVGALLLLGFILLVLFAPFV
                     AGADPYKMDLSGTFQPAGSHGHILGTDNYGRDTFSRLLFGGRTSLLIGIVVVALAAVL
                     GTVLGLLAGYYGGWVDFTVMRLVEVFYAFPFLILVIAVMAILGPSIFNVMWVLGLVNW
                     PSYARLVRGQVLSLKKTEFIQAARTTGARDARIIFQHIMPNSITPIIVQATLGMPSAI
                     LSSAALGFLGFGV"
BASE COUNT          832 a         1687 c         1648 g          949 t
ORIGIN      
        1 acttgaccgc gcgcaggaac tgctcgaact gatcgaacgt agtgatgggc ggcaggccca
       61 gcttcactcg ccagtccttg cggacctgca gcaccacgcc cacgttggtc ccgctggcct
      121 ggggaatggc ccagatcctg ccgttggagg tcacagcctt ccacatctcg cccgggggca
      181 ggctcgacca gccgggtggg ttgttgaaca gcttcttgat gttcggcgcg tacgtgttga
      241 cgttgtccgt gatgtcctgg atggctccgc tggatcggta gttggcgagt gacaccttgt
      301 tccccaccgt gaacaggtcg atcgcctgct gggcggctat ggccgccgtg atctgggtgg
      361 tgtaggaatc cgcgtccttc acgtcggtgt acgtgaactt cacgccggtc tgcttttcga
      421 tgtagtcgtg gatggccttg tactgctcgt cggtggcgac gtggcccatg taggtcatga
      481 cgctcaccgg cgccggctgg gctgcggagg agccggccgc tttctcgccg ctgcccgcgg
      541 caagcgccgc caccgccggc accgccagca ggagcgcgag gaaacccgca actcgtttca
      601 tgttttcccc ctttgtgggc gtcttggcag ccctcagccg gcccgaccgg tgcccggctt
      661 gggggcgcat gggacaccgc gtgctggatc ctaggatacc cagaggtggt gcagggcgcc
      721 aactaccgtg acagagacgc ccgcggtgcc cttctgcgtg cctccggccc gcggtaggat
      781 tccggtgtcg gagcctcgat gacttcgtgc ggcggttgat ccctaggcgc gccggctgcg
      841 gtccatctcc gcgaaggtag tcgccgggag gcctcggtcc cgcacgagct ggaaggtcat
      901 ctccaggaca cgcatggctg gatcgctgag agccaggctc cacggatgcc agatcagggt
      961 cacgtagctc gagtgcgccg cgatggcggc atcgatgaag tagccgttgt tgtactggat
     1021 ctcttcctgg ggcgtccgga cgggatgtgg gggcactgcc gcggcgaatg gcgtcgggaa
     1081 caggacgacc cgttgcggct cctggccaaa gactcggttg ttgtccttca acagattctc
     1141 gtgccagccg tgggcggggt actcgttgat ctcggggtgt ccatccgacg cgtagctcca
     1201 gggctcggcg aggggagcgg ggagggagaa atcggcgccc cagagaacgc tggacacgta
     1261 ccggtagccg gcctccgcga caaggtccag ggcgacggtc gcgccgcgca ggccttccac
     1321 gaacccgcat ccaggacgaa agccgacgca ggggcgcccg aatacgtcct ccacgaccgc
     1381 cttcccctgg cggatctccc gctcgacctg cgcgcggtcg gcggctggcc cgcagatggg
     1441 atgatcgagg agcatgcggt gggagtagct gtgggaggcc acctcgaaga gcgggtcatc
     1501 caacagcgcg cgatattccg acggcgactc ctccagggtt cgccccacga tgaagaacgt
     1561 agcgggcatc cggtaggccc gatgcacctc cacgatccga cggcaggcct cgaggcaggt
     1621 cgggacatgc ggcccgggac gggacgccgc ggacatctcc gtgtcgtagg ctgcgatata
     1681 ccgcgtcatg gaggaagcgt agcggtgccg agagggtgcc gcaaggcgcg ctcgcgcttg
     1741 cgccccggag agctttcccc tataatccgc gccatcgcgg ctccctgagc cgcaggcgat
     1801 acgcaggaag gaaaggaaaa tgacgagaag agcaggagtt ctggtcggtg ccatcgcgat
     1861 gatcgcggtc ctcggcggcg cggcattcgc ggctcccgta tccgggggaa ccctcaagat
     1921 cgccgtcctg agcctcgaga gtctcgagct ctacaagacc gcggcaaacg acatcaccga
     1981 cgcacagagc gtcatcttcg attcgctgtt ccagatcggc aaggacaact tccagcccat
     2041 cccgtgcctg gcatccgggt ggaccaaccc ggatcccacg acgtgggtct tcaccatccg
     2101 caagggcgtc ctcttccagg acgacaaccc ggtcttcccc aagggcaaga gccgcgaggt
     2161 cacggcggat gacgtggtgt actcgatcaa cttcatgatg aagaccagca cggcgtggac
     2221 gctgggaccg gtgaccagcg tcaaggcgct ggaccgctac acggtggaga tcaagaccga
     2281 cagtccgcag ccgttcctcg tgaacgaccc caaccgactc tgccgggtca tgatcgttcc
     2341 caaagaggcg atcgatcagc tcggagcgga cggattcgcg aagtatccga tcggcaccgg
     2401 gccgtacaag ttcaagagct tctctccgga ctcgggcctg ctcctggtga ggaaccccct
     2461 ctaccggctt cccgtctatc cggatgccgt ggagatggtg gtgatcccgg atcccgtggc
     2521 ccagaccctg gccctgcagt ccggcgaggt cgacgtgatc aagcacgtgt tcaacctgga
     2581 aatggtggac accctccaga aggatcccaa cctcatcgtg acccgggcgc ccggtgggtc
     2641 gtatcgaggc atcgggttca acgtgaccgt gccgccgtac aacgtcctgg cggtccgcaa
     2701 gggcctgtcc cagatcctgg acatcgacgc ggcctacaag gcggtgatcg gcaacaaggg
     2761 cgagcgggcc tacggtcagg tgcccccgtg ggtggccttc ggctatgacc ccagcctcaa
     2821 ggacctgtgg agctacaacc ccgcggacgg actcaagctc ctcatggcaa acgggttctc
     2881 caaggtcgcc ggtgaccagt tcctggagtt caacggcaag cccttccaga tccagatcaa
     2941 gaccctggcc ggctcccagg tgcaggcgct cacgatcctg gccacccagc tccgggagca
     3001 agggatcaac gcgagcgtcc tgaccgagga cacggccgtg tgggcggatg acctggtcaa
     3061 gggaaacacc gggatgttct tcgactacag cttcgcgggg accacgggct tccactccct
     3121 gttccacggg agcatgatcg gctccgccaa cacccacttc tacaacaatc ccgcggtgaa
     3181 caccctcctg gatcaggcgc ttaccaaggt gaacatcgac gagctctccg tgctctggaa
     3241 gaaggcgcag cggctggtca tgcaggacgt cgtggggatt ccgttgtact tcgagtacag
     3301 ctacgcggcg gtcaaccgga aggtcaacga cttcgttccg ccgtggggtg ggctgaacct
     3361 cgtgtccatc gagaacaacg tctggctcag taggtagcgc tccggcagcg ggcggccgcc
     3421 tcgtgcgcgc gggacaccga gcgccggggg gccgttcgcg tccgcagaga cccgaatggg
     3481 ccgatacctc cttcgcagag tcctcacgac catcccggtg ctgttcggca tcgtgttcat
     3541 cgtctatgcc atgatctcct tcgttcccgg caacaccatc agcctcatgc tgagcaacga
     3601 gtcggtgaac gtggccaacg tggagaagct gaagatcgag ctgggcctgg acaagccgtg
     3661 gtatacccgg ttcgtcctct ccatggggaa gctggtccgc ggggacctcg ggcgctccat
     3721 gcagtccaac cggccggtgg cggacatgat ccgggagagg atcgggaaca cgttccgcct
     3781 caccgtgttc tcctgtctcg tggccctcct ggtctcggta ccgctgggcg tgctcgccgc
     3841 ggccaggcga agatcggtct tcgactacct ggcaacggcc ctggcgctgc tgggggtctc
     3901 cactccttcc ttctacctgg ggcttctctt gatcctgctg ttcgggttga agctccactg
     3961 gcttcccatc cgagggctgc cctcctacaa cgccacgttc ctgggccaga tccgctacct
     4021 gatcctcccg tcggtcaccc tgggcagcgc cctcacgggc atccttacgc gcctgacccg
     4081 cgcgagcatg ctcgaggcgc tgggccagga cttcgcgcgc acggcgcggg caaagggtca
     4141 gagccaggcg ggcgtcctct tccgccacgc gcttcccaac gcctttctgc cggtgctcac
     4201 gaccatcggc ctgcagttcg gatccctcct gggcggcgcc gtcatcatcg agacgatctt
     4261 ctccctcccg ggcctcggcc ttctggcgct gaacgccgtg aagttccgcg acgtcccgct
     4321 gatccagggg acggtcctgg tcttcgcggt ctgcttcatc gcggtgatgc tcgtcgtgga
     4381 catcctctac gtgttcgtgg acccgcggat ccggctggac tgatatggcg cgttcggacc
     4441 gcaggagagt cgccgttccc cgccttctgc gggtcttcat gcgccatcgg ctggggatcg
     4501 tcggagcgct ccttcttttg ggcttcatcc tcctggtcct cttcgcgccg tttgtggcgg
     4561 gagcggatcc ctacaagatg gacctcagcg gaaccttcca acccgccggg agccacggtc
     4621 atatcctggg caccgacaac tacgggcggg acacgttctc gcggctcctg ttcggcggca
     4681 ggaccagcct cctgatcggg atcgtggtcg tcgcgctggc cgccgtcctg gggaccgtgc
     4741 tggggctcct tgccggttac tacggcggtt gggtggactt caccgtcatg cgcctggtgg
     4801 aggtcttcta cgcgttccca ttcctgatcc tggtcatcgc ggtgatggcg atcctggggc
     4861 ccagcatctt caacgtcatg tgggtcctgg ggctggtgaa ctggcccagc tacgcgcggc
     4921 tggtgcgcgg tcaggtcctc tccctgaaga agaccgagtt catccaggcc gcccggacga
     4981 ccggcgcccg ggatgcccgc atcatcttcc agcacatcat gcccaactcc atcacgccca
     5041 tcatcgtcca ggccaccctc gggatgccca gcgccattct ctcctccgcc gcgttggggt
     5101 ttctgggttt cggcgt
//
LOCUS       DASRZS010000241         6327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4755448, whole genome shotgun sequence.
ACCESSION   DASRZS010000241 DASRZS010000000
VERSION     DASRZS010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4755448"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1734
                     /locus_tag="VFH83_06020"
     CDS             <1..1734
                     /locus_tag="VFH83_06020"
                     /inference="COORDINATES: protein motif:HMM:NF017078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6485954.1"
                     /translation="YRDLEVRQMGRSVDLVTTDIAQELRRIESTGQDWAYWDETARFV
                     DGRNPTFEKVNLADSALSGLDLGFIIFADTRGRVVLAKVINQRTGQAIPASRSFVEEA
                     TEGGALLRPMSGTGIAGVIKVGSRAALIDALPILNSRRELPSHGVLLLGTIIDDREVT
                     GISGRVRFPIEAVPQSDPSVRSLFGRSPPSPPGARELVVRPIDGRTVAGYTPIRDLQG
                     GVGLILRITASREIYAEGVRAALYSTLALVVMGLVTFGVIHLLLGTLILQRLSRITAF
                     MGLLGTGGPLMLRLGTGGRDELAQLSRAVDGLLDTVQKTTDDLREATAQAVVANRVKT
                     EFLANMSHELRTPLNHIIGFTELVADGTAGPVSATQREYLGDVLDSSRHLLSIINDIL
                     DLSKVESGKLELQPARIAVRGLVESSMRMVTEKASKHGVELSLDLSEAPQEVVADERA
                     LKQVLFNLLSNGVKFTPDGGQLRVQVRRCPPASGSGARVEFAVTDTGIGIPPDDLKRL
                     FAPYTRGQASAAAGYEGTGLGLALSKRLVELHGGRIWAESEGPGLGSSFRFVLPLERR
                     QALAVEMSVRR"
     gene            1731..3512
                     /locus_tag="VFH83_06025"
     CDS             1731..3512
                     /locus_tag="VFH83_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6485955.1"
                     /translation="MSGARILVIEDDPLNRKLLRTLLNLGRYQVLEAAEAETGIRMAR
                     EMPPACILMDLRLPGMDGYAATRILKADPALSKVPVVAVTAAVMSGDERRALDAGCDG
                     YIAKPIDTRTFLDNIRQFLHATSQAAAESAQAARKPRVLVVDDEPPVRKVLSDLLVRE
                     GYQVLEARSGTEALQSAHEENPDLILLDVVMADIDGFEVTRRLKGSLRTLGIPIILVT
                     GLSDRGSRTRGIEVGADEFLTKPVDSLELMVRIKSMLRLKVAQDRLLRGVRREGAADE
                     PGAAGEPHDTVVLVAERDGDAARSMECILHGTGCRLSWAADTEDVLAQVSQREVDILV
                     LGTLGSDGSKERELCRLLKEEERTRNLQIIRVSSSHEPGERAQALEDGADEVLSLPLD
                     PDELLVRVRQHAGRKARLDSLLARYRSAANAAASDGLTGLVNNTEFKRLLDIELKRSR
                     RYAYKVSLLFLDLDDFKKKNDTLGHLVGDLFLTEAARVLKSGTRDIDLSARYGGDELA
                     VLLPHTDEAGAAVVAERLRAAIADHPFPGGTAGVEEHVTASIGVACFPGESASPEELL
                     RLADERLYQAKREGKNRIRGPVVQSTG"
     gene            complement(3577..4959)
                     /locus_tag="VFH83_06030"
     CDS             complement(3577..4959)
                     /locus_tag="VFH83_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET6485956.1"
                     /translation="MPTVELSQVEIPDFGAPTVEPEIGAAEYQARITRAKEMARDRGI
                     DILLVYGDREHWSNLTFFTGYDPRFEESLLILGPSDPPILLVGNEGMGFSRKSPIPLD
                     RRLYQQLSLMGQPRGGSKPLEKLLVEAGLRKGREIGIIDWKYFTSEVGPDHELWLCAP
                     DFLVRAIRAVTGTDPRNVTDIAMHPATGLRCINSVDQLAAFEFNSTCTTRAMRRTLEA
                     LRPGMTEYEAVEIAAWNGMPASCHPVMASGPRADWVGSPSMRRLQEGDPVLFSLSGWG
                     SLCARAGFLVRDSGGLPPAIRDYVQKLAAPYFGAVVEWYQAIGIGTPGGELARIIQSR
                     LGDPFFGVSLNPGHLIHLDEWVHSPIAPGSQIPLASGMAIQVDIIPATGGPYFTSNIE
                     DGIALADERLRTELASRWPEVWRRIQARRAFMKDRLGISLKPEVLPFSSIPACLAPFL
                     LSPRMLLRVT"
     gene            5059..>6327
                     /locus_tag="VFH83_06035"
     CDS             5059..>6327
                     /locus_tag="VFH83_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET6485957.1"
                     /translation="MQVKTTLTPESLERGLRDLWALSAEKIRATDRGYDASRGAPVFT
                     SGGVWTSRGWTEWTQGFQYGSAILQYDATGDAEFLESGRRNTLEKMAVHVSHVGVHDH
                     GFNNVSTYGNLRRLMNEGRIPENPSERSFYELALKVSGAVQAARWTAIPDGGFIYSFN
                     GPHSLFVDTLRSLRALAVAHSLGHQLMAENDRKVSLLERLLLHARATARYSIYYGEGR
                     DGYDVPGRVAHESIFNVNDGAYRCPNSQQGYSPFSTWTRGLAWAILGFAEELEYLETV
                     GDTELDPLGGREAARSVLRRAAEVTSEFYIANTASDGIPYWDTGAPGLAHMGNWRERP
                     ADPYNAHEPVDSSAAAIACQGLMRLGHWLRSQGEKTGERLFAAGLTVLARLLEEPYLS
                     RDERHQGLLLHSVYHRPNGWDFIPKGRRVPC"
BASE COUNT         1053 a         2078 c         2191 g         1005 t
ORIGIN      
        1 tatcgggacc tcgaagtccg gcagatggga cgcagcgtgg acctggtcac caccgacatc
       61 gcccaggagc ttcggcgcat cgagtccacc ggccaggatt gggcgtattg ggacgagacg
      121 gcgcggttcg tcgacggccg caatccgacg ttcgagaagg tcaacctcgc cgactccgcg
      181 ctctcgggcc tcgacctcgg cttcataatc ttcgccgata cccgggggcg ggtagtcctg
      241 gccaaggtga tcaaccagcg aaccggtcag gcaatcccgg catcccggtc cttcgtggaa
      301 gaggcgaccg aagggggtgc tctcctgcgg cccatgtcgg gcaccggcat cgccggggtc
      361 atcaaggtcg gatctcgcgc cgccctgatc gatgcgttgc cgatcctgaa cagccggcgg
      421 gagctgccgt cgcatggcgt gctcctgctc gggacgatca tcgacgaccg agaggtcacg
      481 ggtatctccg ggcgggtgcg gttccccatc gaggcggtgc ctcagagcga cccgtccgtc
      541 cgatccttgt tcgggagaag cccgcccagc ccgcccgggg cgcgggagct cgtagtccgc
      601 cccatcgacg gcaggaccgt ggccgggtac acgcccatcc gagacctcca gggcggcgtc
      661 gggctgatcc tgcgcatcac ggcttcccgg gagatctacg cggagggcgt ccgcgccgcg
      721 ctttactcta ccctggcgct ggtcgtgatg gggctggtca cctttggcgt catccatctt
      781 ctcctgggca ccctgatcct gcagcgcctc tcgcggatca ccgcgttcat ggggctcctg
      841 ggcaccggcg gtccgctcat gctgcggctc ggcaccggtg gccgggacga gctggcccag
      901 ctatcccgag cggtggacgg tcttctggac acggtccaga agaccacgga cgacctgcgc
      961 gaggccaccg cgcaggcggt cgtggccaat cgagtcaaga cggagttcct ggccaacatg
     1021 agccacgagc tgcgcacccc tctcaaccac atcatcgggt tcaccgagct ggtcgcggac
     1081 gggacggcgg gccccgtctc cgcgacccag cgggaatacc tgggggacgt gttggacagc
     1141 agccggcatc tcctttcgat catcaacgac atcctcgacc tttccaaggt cgagtccggc
     1201 aagctcgagc tgcagcctgc ccgcatcgcg gtcaggggtc tcgtggagag cagcatgcgg
     1261 atggtcacgg agaaggcctc caagcacggt gtcgagctct cgctggatct gagcgaagcg
     1321 ccgcaggagg tggtggcgga cgagcgggcg ctgaaacagg tgctgttcaa cctcctgtcc
     1381 aacggagtga agttcacgcc ggatgggggt cagctcaggg ttcaggtccg ccggtgtccg
     1441 cccgccagcg ggagcggcgc gcgggtggag ttcgcggtga cggataccgg catcgggatt
     1501 ccgccggatg acctgaagag gctcttcgcg ccgtacacgc gcggccaggc cagcgcggca
     1561 gccggctacg aggggacggg gctgggcctg gcgttgtcca agcggctggt ggagctgcac
     1621 gggggaagga tctgggccga aagcgagggc cccggcctgg ggagctcctt ccgcttcgtg
     1681 ctccccctcg agcggcgaca ggccctcgcc gtagagatga gcgtccgccg atgagtggag
     1741 cccggattct cgtgatcgag gatgatcctc tcaatcggaa gctcctgcga acgctgctga
     1801 acctgggcag ataccaggtg ctggaggcag cggaagccga gacgggcatc cggatggcgc
     1861 gggagatgcc gcccgcctgc atcctgatgg atctgcgtct ccctgggatg gacggctacg
     1921 ccgccacccg catcctgaag gcggatccgg cactgagcaa ggtccccgtg gtggccgtca
     1981 cggctgccgt gatgagcggc gacgagcgac gcgcgctgga cgcgggctgt gacgggtaca
     2041 tcgccaagcc catcgacacc cggaccttcc tggacaacat ccgccagttc ctgcatgcca
     2101 cctcccaggc tgccgccgag agcgcgcagg cagcgcggaa gccccgcgtg ctcgtcgtgg
     2161 acgacgagcc cccggttcgc aaggtgctct cggatctcct cgtacgggag ggctaccagg
     2221 tgctggaggc tcgctcgggg accgaggcgc tacagagcgc ccatgaggaa aaccccgacc
     2281 tgatcctcct ggatgtcgtg atggcggaca tcgacgggtt cgaggtgacg cgacggctga
     2341 agggaagcct gaggaccctt ggaatcccga tcattctggt cacgggcctc tcggaccgcg
     2401 gatctcggac ccggggcatc gaggtcggcg cggatgagtt cctgacgaag ccggtggatt
     2461 ccctggagct gatggtgcgg atcaagtcca tgctgcggct gaaggtcgcg caggatcggc
     2521 tgcttcgagg cgtccgccgg gaaggggctg ccgatgagcc cggcgccgcg ggggagccac
     2581 acgacacggt tgtcctcgtt gccgagcgcg acggagacgc cgcgcggagc atggaatgca
     2641 tcctccatgg aacgggatgc cgattgtcgt gggccgcgga cacggaggac gtcctggccc
     2701 aggtgagcca gcgcgaggtg gacatcctgg tcctggggac actcggatcc gacgggtcga
     2761 aggagcgcga gctctgtcgg cttctgaaag aggaggagag aacgcgcaac ctgcagatca
     2821 tccgggtttc ctcttctcac gagccggggg agcgggccca ggcgttggag gacggcgccg
     2881 acgaggtcct ctcccttccg ctggacccgg atgagctcct ggtccgggtc cgccagcacg
     2941 ccgggaggaa ggcgcgcctc gattccctcc tggcgcgcta ccgctccgcg gcgaacgcgg
     3001 cggccagcga cgggcttacc ggtctggtca acaacacgga gttcaagcgg ctcctggaca
     3061 tcgagctgaa gcgctcacgt cgctacgcgt acaaggtgtc gctcctgttc ctggatctgg
     3121 acgatttcaa gaagaagaac gatacgctgg gacacctggt cggagacctc ttcctcacgg
     3181 aggcggcccg ggtcctgaag agcggaactc gggacatcga tctgagcgcg cggtatggag
     3241 gggacgagct ggccgtcctc ttgccgcaca ccgacgaggc gggcgcggcc gtggtggcgg
     3301 agcgccttcg cgcggccatc gcggaccatc ccttccccgg cgggaccgcc ggcgtggaag
     3361 agcacgtcac cgccagcatc ggcgtggcct gcttccccgg ggaatccgcg tcccccgagg
     3421 agctcctgcg gctcgcggat gaacggctct accaggccaa gcgcgagggc aagaaccgga
     3481 tacgcggccc ggtggtgcag tccaccggat agggggcggc cccggccgtc ccccggtggc
     3541 aggcgcctat cccatcgtcc cgccgggacc gcctcctcac gtcacgcgca acaacatccg
     3601 agggctgagc aggaacggcg cgagacacgc ggggatgctc gagaagggca gcacctccgg
     3661 cttcagcgag atgccgagtc gatccttcat gaaggcgcgc cgcgcctgga tccttcgcca
     3721 tacctcgggc caccgggagg cgagctccgt gcgcagccgc tcgtcagcca gcgcgatccc
     3781 gtcctcgatg ttgctcgtga agtaagggcc tccggtggcg gggatgatgt ccacctggat
     3841 ggccatgccg gaggcgaggg ggatctggga ccccggcgcg atcggagagt gcacccattc
     3901 gtccaggtgg atgagatgcc ctgggttcag ggagaccccg aagaacggat ctcccagccg
     3961 gctctggatg atccgagcga gctcgccgcc gggagtgccg atgccgatgg cctggtacca
     4021 ttccacgacc gctccgaagt acggtgccgc cagcttctgc acgtaatccc ggatggccgg
     4081 gggaagcccg ccggaatccc gaaccaggaa gccggccctc gcgcaaagcg acccccagcc
     4141 ggagaggctg aacagcaccg ggtcgccttc ctggagccgt ctcatggatg ggctgccgac
     4201 ccagtcggcc cggggtccgc tcgccatcac gggatggcag gaggcgggca tgccgttcca
     4261 cgccgcgatc tccactgcct cgtattcggt catgccgggt cgcagggctt ccagggtccg
     4321 cctcatggcg cgcgtggtgc aggtggagtt gaactcgaac gcggcaagct ggtccacgct
     4381 gttgatgcat cgaagtcccg tggccgggtg catggcgatg tcggtgacgt tgcgaggatc
     4441 cgtgccggtg accgcccgga tggctcgtac cagaaagtcc ggggcgcaaa gccaaagctc
     4501 gtgatccggt ccgacctccg aggtgaagta cttccagtcg atgatgccga tctccctgcc
     4561 tttcctgagg cccgcttcga ccagaagctt ctccaggggc ttgcttcccc ctcggggctg
     4621 gcccatcagg ctgagctgct ggtacagccg gcggtccagg gggatggggc tcttgcggga
     4681 aaaccccatg ccttcgttcc ccaccaggag gatcggcgga tcggatggcc ccaggatcag
     4741 aagggattcc tcgaaccgtg gatcatagcc ggtgaagaag gtcaggttgg accagtgctc
     4801 acggtctcca tagaccagca ggatgtcgat cccgcgatcc cgcgccatct ccttggcccg
     4861 cgtgatgcga gcctggtact ccgccgcgcc tatctccggc tccacggtcg gcgcgccgaa
     4921 atccggtatc tccacctggg aaagctcgac ggttggcatg gccgcactgt atcacatcac
     4981 agccgcgtgg acacagccga cgggcagcgc cgcaccgtcc gctggactcc gggcgcggcc
     5041 accggctact ctctgcggat gcaggtcaag acaaccctca ccccggagtc cctggagcgg
     5101 ggtctcaggg acctgtgggc gctctccgcg gagaagatcc gcgcaacgga tcgcgggtat
     5161 gacgccagcc gaggggctcc ggtattcacc agtggaggcg tgtggacatc acgcgggtgg
     5221 accgaatgga ctcaaggctt ccaatacggg tctgccatcc tccagtacga cgccacgggg
     5281 gacgccgagt tcctggaatc cggccgccgc aacacgctgg agaagatggc ggtgcacgtc
     5341 tcgcacgtcg gcgtgcacga ccacggcttc aacaacgtga gcacctacgg caacctgcgc
     5401 cggctgatga acgagggacg catcccggag aatccttccg agcgttcctt ctatgagctt
     5461 gccctcaagg tgagcggcgc cgtgcaagcc gcgcgctgga ccgcgatacc ggacggcggc
     5521 ttcatctact ccttcaacgg gcctcactcg ctgttcgtgg atacccttcg gtcgctgcgg
     5581 gccctcgccg tggcccacag cctgggtcac cagctcatgg ccgaaaacga ccgaaaagta
     5641 tccctgctgg agcgcctcct cctgcacgcg cgggcgacgg cccgatactc gatctactac
     5701 ggcgagggac gggacggtta cgacgtgccg ggtcgcgtgg cgcacgagtc gatcttcaat
     5761 gtcaacgacg gagcctatcg gtgtcccaac tcccagcagg gatactcgcc gttcagcacg
     5821 tggactcggg ggctggcgtg ggccatcctc gggttcgccg aggagctgga atacctggag
     5881 accgtgggag acaccgagct cgatcctctg ggaggtcgtg aggcggcgcg ctcggtgctg
     5941 cgccgcgccg ccgaggtgac ctcggagttc tatatcgcca acaccgcgag cgacggcatc
     6001 ccgtattggg ataccggggc gcccgggctg gcgcacatgg gcaactggcg ggagcggccg
     6061 gcggacccct acaacgcgca cgagcccgtg gacagctccg cggcggccat cgcctgtcag
     6121 gggctcatgc ggttggggca ctggctgcgg tcgcagggag agaagacggg agagcggctc
     6181 ttcgcggccg gcctcacggt cctggcccga ttgctggaag agccttacct cagtcgggac
     6241 gaacgccacc aggggctgct cctgcactcc gtgtaccatc gcccgaacgg ctgggatttc
     6301 attccgaagg gccgcagggt tccgtgc
//
LOCUS       DASRZS010000242        13791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4370437, whole genome shotgun sequence.
ACCESSION   DASRZS010000242 DASRZS010000000
VERSION     DASRZS010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 13791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13791
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4370437"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..801)
                     /locus_tag="VFH83_06040"
     CDS             complement(124..801)
                     /locus_tag="VFH83_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HET6485958.1"
                     /translation="MERRGYRYLNLVTGVFVTCLVVSNIIAVKIVSVAGIVLPAAVVV
                     FPISYIVGDVLTEVYGFGPARQVIWIGFACNAVAVLAMWVGGRLPAAGFWDGQAAYQR
                     ILGYTPRLLGASFMAYLVGEFLNSVVLAKMKMATKGRWLWTRTIGSTLVGELADSAIF
                     ITLSFAGTAGAPDLARLILAQWLFKSAYETVATPLTYWVVGFLKRTESVDHYDYYTDF
                     NPFRLKG"
     gene            complement(859..1404)
                     /locus_tag="VFH83_06045"
     CDS             complement(859..1404)
                     /locus_tag="VFH83_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HET6485959.1"
                     /translation="MAQPEIEGKYFLSYEHIHLSVRALAERILSSGFDPDLMVAIGSG
                     GFIPARILKTFIRRPVFTVGISYYAPDHSHADTPRAIQWIDEVEQKLAGKRVLLVDEV
                     DDSRATLEYCLRELLRHRPAEIAVAVLHNKRKDKKGKLPPEVRRYFAGEELDDKWICY
                     PWDAIDITAHEKISRSGALSR"
     gene            complement(1428..2135)
                     /locus_tag="VFH83_06050"
     CDS             complement(1428..2135)
                     /locus_tag="VFH83_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485960.1"
                     /translation="MLATVLAVLLAATTIAGADPAPLPDRTFSLPKSEVNELFLAYLT
                     GVIAADAYVALDHEQLASMFPELGGDPSQPFRILAGVARVRGEPGPAELVFSLSGEVH
                     VPIPFGLLWYHPISVHASRVVRLGETRYPVRMAGGRQGAPASLAPAFEYRVVDGEAHL
                     HVDSWLVFLTGGLLDEFSVRGAALFRYQGDWYGLVAGVNPHHRVLCWAFDLTRMRLVL
                     SPPEGFLDVAMSLAQQP"
     gene            2254..2607
                     /locus_tag="VFH83_06055"
     CDS             2254..2607
                     /locus_tag="VFH83_06055"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET6485961.1"
                     /translation="MAEASQGYDSDLLRGNTDSLLLFLINEHGQTYGYRLIKEIEERS
                     GGYFRFKEGTVYPALRKLENEGLIRGAWEKLPNGQERRYYTMTDQGREFLQQKLNMWK
                     SFASAMALVMKPSAG"
     gene            2623..3603
                     /locus_tag="VFH83_06060"
     CDS             2623..3603
                     /locus_tag="VFH83_06060"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HET6485962.1"
                     /translation="MDRQVGLFLDEVRSHLHLDLVTERRVLNELYTHFQEKVGDLQDQ
                     GMADGEAIREALASFGDARSIARLMDEAYGRGSWLEALISSQPHLIVAALFATHIWRY
                     PVLLGAAFAAIVIIALMGWRQGTPNWLYSWVGYAVLPLLIFSYVSVDPVAQAITYVFR
                     GRGLTVPLWQLAGLALLYGFTLWLIATASVRVARRDWILVSLMLLPLPVLLVWMVTIT
                     RLDGFYQSFLRGMESGISRWDGGMAYFCLVLGVTTVVFVRMRQRALKVLAIIVVGMIC
                     GALALRNLWGDVGLLSLVGISIGLFLFLTIPFLLRALMGSEGHGKTPLAF"
     gene            3720..4424
                     /locus_tag="VFH83_06065"
     CDS             3720..4424
                     /locus_tag="VFH83_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET6485963.1"
                     /translation="MMARYFYKVLRHLFFPSRLRGRANLTPGEPVIMVANHVGSFGPV
                     SVITSMPVRMYPWVAHEVTDLKTVSRRIQDEFFEQELHLRPPVSAFLARVAGRICVAL
                     MRDIGAIPVYQQSKKIRTTVQLSLRLLEQGKPILVFAEDSTRKINEALCEFCTGFIHL
                     AKLYYDKTKKAILFLPVGVNRKVGGILIGKPIRFDAANPFPREKLRLKSELEASVFTL
                     YQELERDLGEHRVRPA"
     gene            complement(4428..6125)
                     /locus_tag="VFH83_06070"
     CDS             complement(4428..6125)
                     /locus_tag="VFH83_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HET6485964.1"
                     /translation="MRWLDVTQFGASGRKADDARPAIQQAVDACAENGGGVVYLPPGE
                     YTSGTIHLRSHVRFTIEAGATLYSSRDRSDFDTPQEGRVSLFYGEDLENITLEGRGVV
                     DGQAEYEWRLADLDDRSIKENLDELLKTGRQPLRAFLRKDCHGRLVLLERCVDVRIEG
                     LSFVRSPSWTIHPYGCRRLVIDGIYIRTSLREGVWADGIDPDGCSDVRISNCTIETGG
                     DAIAFYSTSTLGPALPCQNSTITNCRLSSASSAIKFCDANENGVRNVAISNCVITDSN
                     RGIAFMSFDGGSVSDVVISNCTIDCRRFDWFWWGDGDPLYFMSRRRYSELHPELGRPD
                     APAGAIRNVSIRDVVARGAGRCLLRGHPERWLEGIRLDNVRLSLVADPEAAYNKEGSG
                     LTIRYARDIALRNVEVSLGDPSPARWKSPLSLEDSVDVLVEGFRGRVPAGSAEPAIAL
                     KNASGIVIRNSRVDPRGATLLLAGIGTRDIVVGTGVIPPEGRIEEEALPGATVEPPRA
                     IDIRRTVVRKTALRRKPAAKGHKSFRKKPKKKAAPKARGKPSSHRAARSPGTGKKHR"
     gene            complement(6193..8031)
                     /locus_tag="VFH83_06075"
     CDS             complement(6193..8031)
                     /locus_tag="VFH83_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485965.1"
                     /translation="MPNPPRRYLVVTVVYLAVVLQLLILAVFARGFSRTIGDAQISGR
                     ASAFSLFGTRDLRDLTVSWNGLSLRFSRATRPPLQGLEPFSGGADIVFADGARLRVLA
                     AADAAASLSISGGTSAGTTASIQIPYSLSGVPEGFSPDRLSWGRAGKHYVLSLPKGAT
                     LDSSAQSVTLTGIPAAATARLLLSQAGAPAPAAVNGMAVAQPPARAVPSRLPDEKSMT
                     TPDQLAAALGGFADAAYKSWTAARLAATGDAWKLPDGSTGFSDGIGVGLAAESIARGT
                     WQAGLQLLDGAFARWQAQNPGATVPLLSSAYAGDLRTFSMNLQARAAAQLDQARSLLA
                     KSDNSLLAMTGLIPLLADHGGGDLLPEALAFLTSRNAGALDPVSSLGLLEGLLDASEL
                     SDPPAAVATTLRQLVDRRIVPLVVATDAGLYYQSPAGRVDVLSSIRAGALLLRAGAVL
                     GSTLDSAVGRDLVASSVAMADSSAALPATLTLKSGQIASRDGALPPESVYGLLPLGRF
                     VPREIPLDRQIGAGISLWTAADLVSADLSGNVATLVLSFPVGIPHYFLVQGVRSFTQI
                     QLHGIPWHSDPTYYRYSDGWSFDGSSGTLSMKLTGRQPQERVVLEF"
     gene            8307..9482
                     /locus_tag="VFH83_06080"
     CDS             8307..9482
                     /locus_tag="VFH83_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HET6485966.1"
                     /translation="MEQSVTRVALAGRQIAIVGTAHVSPESVAEVQRIIETERPDRVC
                     VELDDGRYRSLTEGSGWQNLNIGKVLREGKGFLLLANLVLSSFQKRLGSDVGVRPGAE
                     MLAAVETAKSLGLPVTLCDREISTTLRRAWARTRLWGKSKMLAAMLASVFTSEKLTPE
                     EIEKLKQKDILQEMMDELAGYLPAAKEVLIDERDLYLAERIRSAEGSSVVAVVGAGHV
                     PGIVNRLQSATEPADLSALESIPSRGLLGKSLPWIIPGLVVVLLGLGFLKSGWQMSLN
                     LALKWLLANGGLAALGSLLALPHPLTVLIAFVGAPIATLNPFVGIGMFTGVAEAFLRK
                     PRVRDFENLATDVESFRGFYRNRVTHILLVFFLSSIGGMIGNFIAIPWITALVSGGR"
     gene            complement(9487..10905)
                     /locus_tag="VFH83_06085"
     CDS             complement(9487..10905)
                     /locus_tag="VFH83_06085"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6485967.1"
                     /translation="MVMVSHPTTGLFQAARVEAGADLPGQTPSHGGSRALYYPEPRTR
                     LHRVAAGFPEESNLKAPSNPSNPWKRTLYAISAAEVLAIAGFSTSSPIVPFFLQDLGV
                     ADPMRLKVLTGLVQALPSLSLVFFSPIWGSMADNYGRKPMVLRAMIGGVVVMVLQGLV
                     TAPWQLVVLRTIQGCITGTVAAATILVASIAPKEEIGYSLGFLQMSIFIGASLGPLVG
                     GVIADLFGRRVNFFATAVLLLAAAVLVGRFVDDNFTPPLNKKPVLKSMLPDFRPLASS
                     TALLSLMGVIAADQIAGSVLGPFLPLFIQKISAGSERVGSMTGLVLGLGAVSSALAAV
                     SIGRISFRLGYKRTLVTCMVGASIFMIPQAFSRTPLQLLILRVVSCFFVGGNLPSANA
                     LIAQRTPQGKRGGILGLSSSIASGSNAIGPAIGATIAVAAGFSAVFLTTAGILAAAGI
                     AILLYIREPRSAVTQEQTAHPA"
     gene            10938..11618
                     /locus_tag="VFH83_06090"
     CDS             10938..11618
                     /locus_tag="VFH83_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HET6485968.1"
                     /translation="MKRFVVLAGNIGAGKSTLVRLLCAMLGWKPYYEPVAENPFLEDF
                     YRDMSRWAFHSQVFFLSHRVRSHAVLLGDPYPVVQDRSLYEDAEVFARNLFLHGRLTE
                     REWTTYQELYRTVSALLPAPDLVVYLRASVPTLRRRIDQRGRGLEAGITDGYLSELNE
                     LYESWIGHFTLCPILTVPGDTMDFVAQSRDLESIAAAVVEHLRGSQGILFPEADAGAS
                     RGGEGPAG"
     gene            complement(11622..>13791)
                     /locus_tag="VFH83_06095"
     CDS             complement(11622..>13791)
                     /locus_tag="VFH83_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6485969.1"
                     /translation="DVPVVVLVRLRDTAAAGRALDRGTVQPLLVSRIGPADLALAIRG
                     TRTAAAREAQLARARDQAQLAARSRAEFLADMSHEIRTPLHAIIGHTELLGETHLDAE
                     QKEYTATVQASAEVLLGLVNDILDISRIDAGRLQPERIDFYLPAVVETAVEMSALAAH
                     RKGLELTTFFPPRLPTLVRGDPVRLRQVLVNLVSNAVKFTHGGEIDATVTVLREDEQS
                     ALVEFSVRDTGIGIPAAKIPTLFQSFTQLDSSSSRKYGGSGLGLAISRRLVEVMGGEI
                     GVESTEGKGSRFWFTLPFGKQRVSDQYSAVGADFFGGLRVLVIDDNETARTILVRYLQ
                     SWGCFAEQAPSGPAALELLRARAGGVEAFRLALVDLRMEGMDGWQLASEISADKTINA
                     TSLVLLAPAGFGAEEARLKLLRWFNGYLVKPVRKARLLETIFTVASGTFDLEPVAAED
                     TPEEVLPAGPARVLVADDVASSRELFRAVLEKGGYTVETVENGREAVDRASKEPFDVA
                     FLDVNMPLVGGPEAARALREAGVRTPIIGITASIRAEERELCLASGMNAFLAKPFRRA
                     ELAAVLRTWLPVARAAQSPESWEPEPAATPVVDSEILDFPAAVSAFMGREEVVRGLVK
                     GFLRHLGTRVPDMRAALAQGDAEAIRFEAHAVKGTALNLRANRLAQAAAALERAGRDR
                     KMDEAASILDQVAAASTELSAYVDAGLSLAHSPGSGGSRR"
BASE COUNT         2450 a         4427 c         4547 g         2367 t
ORIGIN      
        1 tctggactgc ggttggggaa cgcttccagt gcgcgtgagg gtctggcctt cttccgcttc
       61 agtatggtca gacccgcccc cagcgggtct accttccggg ctggctgctt cgtttccgcc
      121 tcctcacccc ttcaatcgaa aggggttgaa atccgtgtaa tagtcgtaat ggtctacgct
      181 ttcggtacgc ttcaggaaac ccacgaccca gtaggtgagc ggcgtggcga ccgtctcgta
      241 ggccgacttg aaaagccatt gggcaaggat caggcgggcc agatccggtg ccccggccgt
      301 gccggcaaag gacagcgtga tgaagatggc ggaatccgcc agctctccga ccagcgtgct
      361 gccgatggtt cgggtccaca gccatcggcc cttcgtggcc attttcatct tggccagcac
      421 cacggagttg aggaactctc ccaccaggta cgccatgaag gaagccccga gcagccgggg
      481 ggtgtagccg agaatgcgct ggtaggcggc ctggccgtcc cagaagccgg cggccggcag
      541 cctcccgccc acccacatgg ccagcaccgc caccgcgttg caggcgaatc cgatccagat
      601 cacctgccgg gccgggccga atccgtagac ctccgtgagc acgtccccca cgatgtagct
      661 gatcgggaag acgaccacgg cggcgggcaa cacgatgccg gcgacggaaa cgatcttgac
      721 cgcaatgatg ttcgagacga ccaggcaggt cacgaaaaca cccgtgacca ggttcagata
      781 ccggtagccg cgtcgctcca cgccgacgcc tccatcctgc gagctcacgg aagcagcccc
      841 gggacgaggc ggcgcgtctc agcgggacaa cgcgccggaa cgggagatct tctcatgggc
      901 agtgatatcg atggcatccc acgggtaaca gatccatttg tcatccagct cctccccagc
      961 gaagtaccgg cggacctcgg gaggcagctt ccctttcttg tcttttcgct tgttgtgcag
     1021 cacggccacc gcgatctccg ccggtcgatg gcgcagcagc tcccgaagac agtactccag
     1081 ggtggcgcgg gagtcgtcca cctcgtccac caggagcacc cgttttccgg ccagcttctg
     1141 ttccacctcg tcgatccact ggatggcccg gggggtatcc gcgtgagagt gatcgggcgc
     1201 gtagtaggag atcccgacgg tgaacaccgg ccgacggatg aaggtcttga ggattcgagc
     1261 cggaataaac ccgccgctgc cgatcgccac catcaggtcc gggtcgaatc ccgaagagag
     1321 aatacgctca gcaagagccc gaacgctgag gtggatgtgc tcgtaggaga ggaagtattt
     1381 cccctcgatt tccggctgag ccatgcaggc ggagagaata agggcaatca cggctgttgc
     1441 gcaagcgaca ttgcgacatc caggaatccc tcgggcggcg agagcaccag tctcatccgg
     1501 gtcagatcga acgcccagca gagcactctg tggtgcgggt tcacgcccgc caccagaccg
     1561 taccagtcac cctggtatcg gaagagcgcg gcgccgcgga ccgagaactc atccaggagg
     1621 cctcccgtca ggaagacgag ccaggagtcc acgtgcaggt gggcctctcc gtcgacgacg
     1681 cggtattcga acgccggtgc gagggaagcg ggcgctccct gtcgacctcc cgccatgcgg
     1741 accggatacc gcgtctctcc aaggcgaacc acccggctcg catgcacgga gatggggtga
     1801 taccagagaa gaccgaaggg gatcgggacg tgcacctccc cggataggga aaacacgagc
     1861 tccgccgggc ccggctctcc tcgaacccgc gccaccccag ccaggatccg aaatggctgg
     1921 gaggggtctc ccccgagctc cgggaacatg gaggccaatt gttcgtgatc cagcgccacg
     1981 tatgcatccg ccgcaatgac ccccgtgaga taggcaagaa acagctcgtt cacctcgctc
     2041 ttcgggaggc tgaaggttcg gtcgggcagc ggcgcgggat cggcacccgc gatggtggtc
     2101 gctgcaagca gaactgccag gaccgtggcc agcacccgga cagtatagca tcgaccgggc
     2161 gtccgggtgc gcgccgcgga gacgggatcc cagccgggca tgtcttgaca gcgttgcggg
     2221 ggcgtcacta ccatgaatct gagaggtgtt tgcatggcgg aggcttcgca gggttacgat
     2281 tccgaccttc ttcgagggaa caccgattcg ctgctgttgt tcctgatcaa cgagcacggc
     2341 cagacctacg gataccggct catcaaggag atcgaggagc gaagcggagg ttacttccgg
     2401 ttcaaggaag gcaccgtcta tcccgcgctg aggaagctcg aaaacgaggg cctgatccgg
     2461 ggcgcttggg agaaactccc caacgggcag gagcgtcgct actacaccat gacggaccag
     2521 gggcgggagt tcctccagca gaagcttaac atgtggaaga gcttcgcgag tgccatggcg
     2581 ctggtgatga agccctcggc aggctgaagt ggttggtggg ccatggaccg gcaggtgggt
     2641 cttttcctcg atgaggtacg ctctcaccta cacctggatc tggtcaccga acgtcgggtc
     2701 ctgaacgagc tgtacaccca cttccaggag aaagtgggcg atctccagga ccaggggatg
     2761 gcagacgggg aggcaatccg ggaagctctg gcgtccttcg gggatgcccg ttcgatcgcg
     2821 cggctgatgg acgaggcgta cgggagaggg agttggctgg aggcgctcat cagcagtcag
     2881 ccccatctca tcgttgccgc cctctttgcc acccatatct ggcggtatcc cgtcctgctg
     2941 ggcgcggcgt tcgctgccat cgtcatcatc gccttgatgg gctggcggca gggtaccccc
     3001 aactggctct actcctgggt agggtatgcc gtcctgcccc ttctgatctt cagctatgtt
     3061 tcggtggatc cggtcgcgca ggccatcacg tatgtctttc gcggccgggg gctaaccgtc
     3121 ccgttgtggc agctcgccgg actggccctc ctgtacgggt tcaccctgtg gctgatcgcg
     3181 accgcctccg tgcgagtggc gcgccgggac tggatcctgg tttcgctcat gttgcttccc
     3241 ctgcccgtgt tgctggtctg gatggttacc atcacgaggc tggacggatt ttaccagagc
     3301 ttcctacgcg ggatggagtc tgggatcagt cgatgggacg gggggatggc gtacttctgc
     3361 cttgtcctcg gggtgaccac ggtcgtcttc gtccgcatgc ggcaacgcgc cttgaaggtc
     3421 ctggcgatca tcgtggtcgg gatgatctgc ggcgccctgg cgctgcggaa cctttggggg
     3481 gatgtcgggc tgctttccct cgtgggcatc tcgatcgggc tcttcctctt cctgacgata
     3541 ccattcctcc tgagggccct gatgggctcg gaaggccacg gcaagacgcc gctggccttt
     3601 tgagccccag tcgctcttct tcccgtatcg gtccaccacc cagtccgggc gagcttgaca
     3661 cctggtatgg gcctcggtag tattgcatcg gctgcctatg taacgacagc gtccgttcca
     3721 tgatggcacg ttacttctac aaggtgcttc gccatctctt cttcccgtcc cggctgcgcg
     3781 gccgggccaa cctgaccccc ggggaaccgg tcatcatggt agcgaaccac gtcgggtcct
     3841 ttggtcccgt ctccgtgatc acttccatgc ccgtccgaat gtacccgtgg gtggcgcatg
     3901 aggtcacgga tctgaagacc gtctcccgtc gcatccagga cgagttcttt gagcaggagc
     3961 tgcacctgcg gccacccgtc agcgcctttc tggcccgagt agcggggagg atctgcgtcg
     4021 ccctgatgcg ggacatcggg gcgatccccg tctaccagca gagcaagaag atccggacca
     4081 ccgttcagct cagcctgagg ctgttggagc aggggaaacc gattctcgtc tttgcggaag
     4141 actcgacccg gaagatcaac gaagccctct gcgagttctg caccgggttc atccacctgg
     4201 ccaagctcta ctacgacaag accaagaagg ccattctctt tctgccggtc ggggtgaacc
     4261 gcaaggtcgg aggcattctg atcggcaaac cgatccggtt cgacgccgcc aatcccttcc
     4321 ctcgggagaa gctacggctg aagagcgagc tggaagccag cgtcttcacc ctgtaccagg
     4381 agctggaacg ggatctggga gagcacaggg tccgtcccgc ctgaccgtta tcggtgcttc
     4441 ttgccggtcc cgggggaccg ggcggcgcga tggctcgatg gctttcctcg cgccttcggc
     4501 gcggccttct tcttgggttt cttgcgaaaa ctcttgtgcc ccttggcggc gggcttccgc
     4561 cggagggcgg tcttgcggac gacggtccgc cggatgtcga tggctcgggg cggctcgacc
     4621 gtcgcacccg gcagtgcctc ctcctcgatt cggccttcgg gtggaatcac tccggtaccg
     4681 acgacgatgt ccctggttcc gatgcctgcc agaagcaggg tggcgccccg cgggtccacc
     4741 cggctgtttc ggatcacgat cccgctcgca ttcttcaacg cgattgcggg ttcggccgat
     4801 cccgccggga cgcggcctcg aaagccttcc accagcacgt ccacggaatc ttccagggac
     4861 aaggggctct tccatcgcgc aggcgaagga tcgccaaggc tgacttcgac gttgcgaaga
     4921 gcgatgtccc tcgcgtaccg gatggtgagc ccgcttcctt ccttgttgta ggcggcttcg
     4981 ggatcagcga ccagggacag ccggacgtta tccaggcgga ttccttccag ccagcgttcg
     5041 gggtgccccc gcaggaggca tcggccggca ccgcgcgcta cgacgtcccg gatcgacacg
     5101 ttgcggatgg ctcccgccgg cgcgtccggc cggccgagct cggggtgcag ctcggagtag
     5161 cgtcggcggg acatgaagta caacgggtcg ccgtcgcccc accagaacca atcgaaacgc
     5221 cggcagtcga tcgtgcaatt ggagatcacc acgtcgctca cggaaccgcc gtcaaagctc
     5281 atgaaggcga tcccccggtt ggagtccgta atgacacagt tgctgatggc gacgttgcgc
     5341 acgccgttct cgttcgcgtc gcagaacttg atggcactgg aggccgagga gagacggcag
     5401 ttcgtgatcg tgctgttctg acagggcagc gccggcccca gggtggaggt ggagtagaac
     5461 gcgatggcgt cgccgccggt ttcgatcgta cagttcgaga tccgtacgtc ggagcatccg
     5521 tccggatcga taccgtcggc ccagactcct tcccggagac tcgtgcggat atagatgccg
     5581 tcgatcacca ggcgccggca cccatagggg tggatggtcc atgatgggga acgcacgaac
     5641 gagagccctt cgatgcgcac gtcgacacag cgctccagga gcaccaggcg gccgtggcaa
     5701 tccttgcgga ggaacgcccg tagcggttgc ctgccggtct tcagaagctc atccaggttc
     5761 tccttgatgg aacgatcgtc cagatcggcg agacgccatt cgtattccgc ctggccatcc
     5821 accaccccgc gtccctccaa cgtgatgttc tccaggtcct caccatagaa cagagaaacg
     5881 cggccttcct ggggtgtgtc gaagtcggat cggtctcgag aggagtacag ggtggcaccc
     5941 gcctcgatgg tgaagcgtac gtggcttctc aggtggatcg tgccggaggt gtactctccg
     6001 ggcggcagat agaccacgcc gccgccgttc tcagcgcatg catccaccgc ttgctggatc
     6061 gccggccgcg cgtcgtccgc cttcctgccg ctcgctccaa actgcgtgac atccaaccat
     6121 ctcatgggga cactgtagcg tggctttggc gcgccgtaaa tggcgggccc gtccgaccga
     6181 tacgcccgag ggctagaact cgagtacgac gcgctcctgc ggctggcggc cggtcagctt
     6241 catggacagg gtcccgctgg atccgtcaaa agaccatcca tcggagtacc ggtagtaggt
     6301 ggggtcgctg tgccacggga ttccgtggag ctggatctgc gtgaatgatc gcacgccctg
     6361 gaccaggaag tagtggggaa taccgacggg gaaggaaagc accagcgtgg cgacgttgcc
     6421 cgagagatcg gccgagacca gatccgcagc ggtccagagg gagatgccgg cgccgatctg
     6481 gcggtccagc gggatctccc gcggaacaaa gcggcccagc ggcaggagcc catagaccga
     6541 ctccggcggc agcgcgccat cccgggaggc gatctggccg gactttaggg tgagggtcgc
     6601 cggcaacgcc gctgaggagt ccgccatcgc tacgctggag gcgaccagat cccgacccac
     6661 ggcggaatcg agcgtggagc caaggaccgc tcccgcgcga aggaggagag cgccagcacg
     6721 gatcgaggaa agaacatcca cccggccggc agggctctgg tagtacaatc cggcatccgt
     6781 ggcgaccacc agcggcacga tccgtcgatc gaccagctgc cggagggttg tggcgacggc
     6841 ggcaggcggg tccgagagct cgcttgcgtc caggagacct tcgagaagac ccaggctcga
     6901 gaccgggtcg agggcgccag cgttgcggct tgtcagaaac gccagggctt ccggtagcag
     6961 gtcgccgccc ccgtgatccg ccaggagcgg gatcagacca gtcatggcca ggaggctgtt
     7021 gtcggatttg gccagaaggg accgtgcctg gtcgagttgg gcggcggcgc gggcctggag
     7081 attcatcgag aaagtccgaa ggtctcccgc ataggcggag gagaggagcg ggaccgtggc
     7141 gccaggattc tgcgcctgcc agcgagcgaa ggcgccgtcc agaagctgca gaccagcctg
     7201 ccaggtcccc cgtgcgatcg attcggccgc aagcccgacg ccaatcccgt cggagaaacc
     7261 ggtgctgccg tccggcaact tccaggcatc tccggtggcg gccagacgcg cggcggtcca
     7321 gctcttgtac gctgcatccg cgaaccctcc gagggcggcg gcaagctgat ccggcgtggt
     7381 catggacttc tcgtccggca gtcgggacgg gaccgcacgg gccggcggct gcgcaaccgc
     7441 catcccgttg acggctgcag gcgccggcgc gcctgcctga ctgagaagca gccgagcagt
     7501 cgcggctgcc gggatccccg tcagggtgac cgactgcgcg gagctgtcca gggtggctcc
     7561 tttggggaga gacaggacgt aatgtttgcc tgccctgccc cagctcagac ggtccggcga
     7621 aaacccttcc ggcacgccgg atagactgta cggaatctgg atggaggcgg tcgtgccggc
     7681 actcgtcccg ccgctgatag acagtgacgc cgcggcatcc gcggccgcca gaactcgaag
     7741 gcgagcgccg tcggcgaaga cgatgtccgc ccctccggag aatggctcga ggccctgcag
     7801 gggaggtcga gtggcgcggg agaaccgcaa agaaagtccg ttccaggaaa cggtgagatc
     7861 ccgaaggtct cgggtgccga agagggaaaa cgcggaggct cggccggaga tctgcgcatc
     7921 tccgatggtc cgggagaacc cacgcgcgaa gactgccagg atcaggagct gcagcactac
     7981 cgccagatac acgaccgtga ctaccagata gcgtcttggc gggttcggca tcggctgcgg
     8041 attctaccaa gggggggtga aatatgcaac caacttcacg ttcccagccg gggcgatgct
     8101 ctaccgggat accctggctt tccacgatct ggcggccgac gtcgcgctat cgaaagtccc
     8161 ccgaccatcg tggtaccatg acgcggcgct catctcgttc ggagtgcccg ggcaagcgcg
     8221 ctttccatgg cgcggcgatc ggtcccgtga aggatgcccc cctcggcatg gaggcctctg
     8281 cagtggaagg ctcggcagga gaaggaatgg agcagtcagt tacccgggtt gcgcttgcgg
     8341 gcagacagat cgccatcgtc ggcaccgccc acgtatcccc cgagagcgtg gcggaggtgc
     8401 agcggatcat cgaaacggag aggccggatc gagtctgtgt cgagctggat gacggacgct
     8461 accgctcgct cacggaggga agcggatggc agaatctgaa catcgggaag gtcctgcggg
     8521 agggtaaagg cttcctgctg ctcgccaacc tggtcctctc ctcgttccag aagcggttgg
     8581 ggtccgacgt cggggtgaga ccgggcgcgg aaatgctggc cgccgtggag acggcaaaga
     8641 gcctcggcct gccggttacg ctgtgcgacc gcgagatctc cacgaccctg cgacgggcgt
     8701 gggcacggac ccggctctgg ggcaagagca agatgcttgc cgcgatgctc gcctcggtct
     8761 tcacgtcgga gaagctcacc ccggaggaga tcgagaagct caagcagaaa gacattctgc
     8821 aggagatgat ggacgagctg gcagggtatc ttccggcagc caaggaggtc ctcatcgacg
     8881 agagagacct ctacctggct gagaggatac gctcggcgga aggctcgtcg gtagtggccg
     8941 tggtcggtgc cggtcacgtt cccggtatcg tgaatcggct gcaatcggca accgagcctg
     9001 ccgatctgtc cgcgctcgag agcatcccct cccgcggact gctcggcaaa tcccttccct
     9061 ggatcatccc cggcctggtg gtcgtgctcc tggggcttgg gttcctcaag tccggctggc
     9121 agatgagcct caatctggcc ctgaagtggc ttctggccaa cggcggtctg gcggccctgg
     9181 ggtctctcct ggctctgccc catccgctca ccgtgctcat cgcgttcgtg ggtgcgccca
     9241 tcgctacgct caacccgttc gtcggtatcg gtatgttcac gggggtggcg gaggctttcc
     9301 tccgaaagcc ccgagtccgg gatttcgaga atctggccac ggacgtggag agcttccgcg
     9361 gcttctaccg caaccgagtc acccacatcc tcctggtctt cttcctctcc agcatcggcg
     9421 gcatgatcgg aaacttcatc gccattccct ggatcaccgc gctggtgagc ggaggtcgat
     9481 agggactcag gccgggtgcg cggtctgctc ctgcgtcacg gccgatcgcg gctcgcggat
     9541 gtagagcagg atcgctatgc ccgccgcggc gaggatgccc gccgtggtga ggaacacggc
     9601 ggaaaagccg gcggcaacgg cgatggtcgc gccgatggca gggccgattg cgttgctgcc
     9661 ggaggcgatg gagctggaga gccccaggat tcccccgcgc tttccctgag gcgttcgttg
     9721 cgcgatcagc gcgttggcgg agggcaggtt cccgcctacg aagaagcagc tcacaacgcg
     9781 cagaatcagc agctgcagcg gcgttcgaga gaacgcctgc gggatcatga agatcgaagc
     9841 acctaccatg caggtgacca gggtgcgctt gtatccaagg cgaaagctga tcctgccaat
     9901 gctcacggcg gccagcgcac tggagaccgc tcccaaccca aggaccagtc cggtcatcga
     9961 gcccacccgc tcggagccgg cgctgatctt ctggatgaac aacgggagga agggcccgag
    10021 caccgagccc gcgatctggt ccgcggcgat gacccccatc agcgacagga gcgccgtgga
    10081 ggatgccaga ggccggaaat ccggcagcat gctcttcagc accggcttct tgttgagtgg
    10141 gggcgtgaag ttgtcgtcta cgaatcggcc caccaggacc gcggcggcca ggaggagcac
    10201 tgcggtggcg aagaagttca cccgtctgcc gaagagatcc gcgatcaccc cgcctaccag
    10261 cggtcccagg gaggcgccga tgaagatcga catctggagg aagccgaggc tgtagccgat
    10321 ctcctccttc ggagcaatcg acgccaccag gattgtcgcg gcggcgaccg ttcccgtgat
    10381 gcagccctgg atggtcctca acaccacgag ttgccagggg gcggtgacca gaccctggag
    10441 caccatcact accacgcccc cgatcatggc gcgaaggacc atgggctttc tcccgtagtt
    10501 gtccgccatg ctgccccaga tcggagagaa gaagaccaga ctcagggacg ggagggcctg
    10561 caccagaccc gtgaggacct tcagcctcat gggatccgca acgccgagat cctggagaaa
    10621 gaagggaacg atcggcgatg aggtcgagaa tccggcgatg gccagcacct cggcggcgct
    10681 gatggcatac agcgttcgtt tccaggggtt ggaaggattg gacggtgctt tcaggttcga
    10741 ctcctcggga aaaccagcgg ctactcggtg caggcgggtg cgcggctcgg gatagtatag
    10801 ggcgcgggag cctccatgag aaggggtttg tcccggcaga tcggcgccag cctcgaccct
    10861 ggcggcctgg aaaagacccg tggtagggtg gctcaccatg accatgttgg tgacgggccc
    10921 gagcagaggt tcattcggtg aagcggttcg tcgttctggc cggtaacatc ggggcgggaa
    10981 agtccacctt ggtccggctc ctctgcgcga tgctggggtg gaagccgtat tacgagccag
    11041 ttgccgagaa tcccttcctt gaggacttct accgcgacat gagccgctgg gccttccatt
    11101 cccaggtgtt cttcctttcc caccgggtcc ggtcgcatgc cgtgctgttg ggagacccct
    11161 accccgtggt ccaggaccgc tccctctacg aagacgccga ggtctttgcc cgaaacctgt
    11221 ttctccatgg tcggctcacc gagcgggagt ggaccaccta ccaggagctg tatcggaccg
    11281 taagcgcgct tcttcccgcg ccggacctcg tggtgtacct gcgcgcctcg gtgccgacgc
    11341 ttcgaaggcg aatcgaccag cggggacgcg ggctcgaggc gggcattacg gacggttatc
    11401 tgtccgagct caacgagctg tacgagagct ggatcgggca cttcaccttg tgccccatcc
    11461 ttaccgtgcc cggcgatacc atggacttcg tcgcgcagag ccgggatctg gagtctatcg
    11521 ccgccgccgt ggtcgaacac ctgcggggct ctcagggaat cctgtttccc gaggcggatg
    11581 ccggcgcctc gcggggaggc gaaggcccgg cggggtaggc cctacctcct ggagccgcca
    11641 gaaccgggcg aatgggcgag cgagagcccg gcatcgacgt atgcggagag ctccgtggag
    11701 gccgctgcga cctgatccag gatcgaggcc gcctcgtcca tcttgcggtc ccgacccgcg
    11761 cgctccaaag ccgctgctgc ctgcgcgagc cggttggcgc gcagattcaa ggcggtgcct
    11821 ttgaccgcgt gggcctcgaa ccgaatggcc tccgcgtccc cctgggcgag cgcggcgcgc
    11881 atgtccggta cccgcgtccc caggtgcctc aggaacccct tgacgagccc ccggaccact
    11941 tcctctctcc ccatgaaagc ggaaaccgcg gccgggaagt ccaggatttc ggagtccaca
    12001 accggcgtcg ccgccggttc gggctcccac gactccgggc tctgcgccgc acgggccacc
    12061 ggcagccacg tgcgaaggac ggccgcgagc tcagctcgcc gaaagggctt cgccaggaat
    12121 gcattcatgc cgcttgccag gcagagctcc cgctcctcag cccggatgct cgcggtgatg
    12181 ccgatgatcg gcgtgcgcac cccggcttcc ctcaaagccc gcgcagcctc cggacctccg
    12241 accaggggca tgttcacgtc caggaacgcg acatcaaagg gttctttgct ggctcgatca
    12301 acggcctctc ggccgttctc gaccgtctcc acggtatagc cgcccttctc caggacggcc
    12361 cggaagagct cccggctgga ggccacgtcg tccgccacga gtacccgcgc gggaccggcc
    12421 ggcaggacct cctccggggt gtcctccgcg gccaccggtt ccaggtcgaa cgtgcccgag
    12481 gcgaccgtga aaatcgtctc cagaagccgc gccttgcgca ccggcttgac cagatacccg
    12541 ttgaaccagc gcaggagctt caggcgcgcc tcttcggcgc cgaaccccgc gggcgccagg
    12601 agcacgagac tggtcgcgtt gatcgtcttg tccgcgctga tctcgctggc cagctgccag
    12661 ccgtccatcc cttccatgcg aaggtccacg agcgcgagcc ggaacgcctc gaccccaccc
    12721 gctcgggcgc gcagaagctc caaggccgcc ggtccgctcg gggcctgctc ggcgaaacaa
    12781 ccccaggact ggagatatcg aaccaggatc gttcgagccg tttcgttgtc atcgatcacc
    12841 aggacgcgaa gcccgccaaa gaagtccgcg ccgacggccg agtactggtc gctcacccgc
    12901 tgcttcccga acggcagggt aaaccagaat cgagagccct tcccctcggt gctctccaca
    12961 ccgatctcac cgcccatcac ctccacgagc cgtcgggaga tggccaggcc caatcccgaa
    13021 ccgccgtact tgcgcgagga ggaggagtcg agctgggtga aactctggaa cagagtcggg
    13081 atcttcgcgg ctgggatccc gattccggtg tcccggacgg agaactcgac cagcgccgat
    13141 tgctcgtcct ctcgaaggac cgtgacggtc gcatcgatct cgccgccatg ggtgaacttc
    13201 acggcgttgc tcaccaggtt caccaggacc tgccgcaggc gcaccgggtc gccccggacc
    13261 agggttggga gtcggggcgg aaagaacgtg gtgagctcca gtcccttgcg gtgggccgca
    13321 agggcgctca tctcgacggc cgtctcaacg acggcgggga ggtagaaatc gatccgttcc
    13381 ggctggaggc ggccggcgtc gatgcgggag atgtccagga tatcgttcac aaggcccaga
    13441 agcacctcag cggaggcctg cacggttgcc gtgtactcct tctgttcggc atccaggtgc
    13501 gtctcgccga ggagctccgt atgcccgatg atcgcatgca gaggggtccg gatctcgtgg
    13561 ctcatgtcgg ccaggaactc cgcgcgggag cgagcggcca gttgcgcctg gtcccgggcg
    13621 cgagccaatt gtgcctcccg ggccgcagcc gtacgggtgc ctcggatggc cagcgccagg
    13681 tccgcgggtc cgatccgaga gaccaggagc ggctggaccg ttcctcggtc cagcgctctc
    13741 cccgcggcgg ccgtatcacg caggcgcacc aggacgacga ccgggacatc g
//
LOCUS       DASRZS010000243         3072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_908769, whole genome shotgun sequence.
ACCESSION   DASRZS010000243 DASRZS010000000
VERSION     DASRZS010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908769"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFH83_06100"
     CDS             <1..384
                     /locus_tag="VFH83_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6485970.1"
                     /translation="AIQVDPGPELRVLADRDALGILFGNLVENAVKYGKEGGRVEVSL
                     ERSGRYATVRVQDDGIGISPEDSQKVFDEFYRARTEQTAAIAGTGLGLSLVKRLAELH
                     EGTVRVQSTLGEGSEFRVDLPLLTY"
     gene            521..2821
                     /locus_tag="VFH83_06105"
     CDS             521..2821
                     /locus_tag="VFH83_06105"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET6485971.1"
                     /translation="MPSLANATAFEVVALWVVLLIALLGLAYALLLRRQVMRSDKGTP
                     KMIEVWNAIRQGADAYLGRQLRVILPLIVVLAAVLFLSVYVVPPSDESLKRFSQFSSD
                     TVRLIIGLARACAFVMGATFSLMVGQFGMRMAVQGNVRVASASRRSFAEALRIAYRTG
                     TITGMLTDGLGLFGGTVIFLIFGIASPDVLLGFGFGGTLLALFMRVGGGIYTKAADVG
                     ADLVGKVEAGMPEDDPRNAAVIADLVGDNVGDCAGMAADIFESYEVTIVSGMILALAL
                     VALTGDFKWLVFPLLVRGIGVFSSIIGTYLVRGSRKEGAPGNALGQINRGFYSSAAIS
                     VVAFGALAFFYMHEWRAFLSVLVGIVLAIVLDEVTKYFTHTEHGPVKSIAASSRTGSA
                     TLILRGLGIGFESAVWQILVIALTIGAALLIYWGQPVSYVLYGVAMTGIGMLTLTGNN
                     VAMDSFGPISDNANGIGEMAALDPKARQIMADLDAVGNTTKAITKGVAIGSAVIAAVS
                     LFGSFLTDVSKVQAQMGILENLRMISTGIRVSVPSVFIGMLIGGAVPWLFSSLTINAV
                     ARAASLIVVEVRRQFRLPGLMEGKIQPDYAQAVGICTAAAQRELIGLSLISILTPLIV
                     GMLLGVEALGGFLAGAILSGQLLAVFMSTAGGAWDNAKKTIEDGLYGGKGSEAHKASV
                     VGDTVGDPLKDTSGPALNPMLKVINLVSVLAAPILIKFTGASVGLVVALVVCVIVVFG
                     AVVFSKRGTLNLRKRSADAGFYAEGS"
BASE COUNT          458 a          987 c         1018 g          609 t
ORIGIN      
        1 gccattcagg tcgacccggg gcccgagctg cgagtgctcg cggatcgcga tgcgctcggg
       61 atcctcttcg ggaatctcgt ggagaacgcc gtgaagtacg ggaaggaggg tggaagggtc
      121 gaggtgagcc tggagaggtc gggacggtat gccacggtcc gcgttcagga cgacggcatc
      181 gggatctcgc cggaggactc ccagaaggta ttcgacgagt tctaccgagc ccgcacggag
      241 cagaccgctg ccatcgcggg gacgggtctg ggcctcagcc tggtgaaacg cctggcggag
      301 ctgcacgagg ggaccgtccg ggtgcaatcc acgctggggg aggggagtga gttccgggtg
      361 gacctcccgc tcctgaccta ttgacacccc ctctacccct ttgttacatg tgtctacaat
      421 tccgctctct gctcgcgttc tgtcccaaag cctgacagga gcatatgtgc gcgctggccc
      481 gccccgcggc gctcacggat aggaaggagg ggtattcgac atgccaagtc tggccaacgc
      541 gacggccttt gaggtggttg ccctctgggt cgtccttctc atcgccctgc tgggccttgc
      601 gtacgccttg ctcttgcgcc gccaggtcat gcgctcggac aagggcacgc ccaagatgat
      661 cgaggtatgg aacgccattc ggcagggcgc ggatgcctat cttggccgtc agctccgcgt
      721 gatcctcccc ctgatcgtcg tcctggccgc ggtcctgttc ctctccgtgt acgtggttcc
      781 ccccagcgac gaatccctca agcgcttctc ccagttcagc tcggacaccg tgcgtctgat
      841 catcggtctg gcccgcgcct gtgcattcgt catgggcgcc accttctcct tgatggtcgg
      901 ccagttcgga atgcggatgg ccgtccaggg caacgtacgc gtggcatccg cctcccggcg
      961 cagcttcgct gaggcgctgc ggattgccta ccgcaccggt accatcaccg ggatgctcac
     1021 cgatggcctg gggctcttcg gcggaacggt catcttcctc atcttcggga tagcctcccc
     1081 ggacgtgctc ctgggcttcg gtttcggcgg gaccctgctg gctctcttca tgagggtagg
     1141 aggcgggatc tacaccaagg ccgcggacgt cggcgccgac ctggtcggca aggtcgaggc
     1201 gggcatgccc gaggatgacc cccgcaacgc ggcggtcatc gcggacctgg tgggagacaa
     1261 cgtcggagac tgcgcgggaa tggccgcgga catcttcgag tcctacgagg tgaccatcgt
     1321 ctccggcatg atcctggcgc tggcgctggt ggctctgacc ggggacttca agtggctcgt
     1381 tttcccgctc ctggtgcgcg ggatcggagt gttctcctcc atcatcggga cctacctggt
     1441 gcggggaagc aggaaggagg gggctcccgg caacgcgttg gggcagatca accgggggtt
     1501 ctacagctcc gcggcaatct ccgtcgtggc cttcggcgcg ctcgccttct tctatatgca
     1561 cgaatggcgg gcgttcctgt cggtcctggt gggcatcgtc cttgccatcg tccttgacga
     1621 ggtgaccaag tacttcaccc acaccgagca tggtccggtg aagagcatcg ccgcttcgtc
     1681 gcgaacgggc tcggccacgc tgattctccg cggtctgggg atcgggttcg agtccgccgt
     1741 gtggcagatc ctcgtcatcg cgctcaccat cggtgcggcc cttctcatct actggggtca
     1801 gcccgtgtcg tacgtgctct acggagtggc catgacgggc atcgggatgt tgaccctgac
     1861 cggcaacaat gtggccatgg actccttcgg gcccatctcg gataacgcca acgggatcgg
     1921 cgagatggcc gctctggacc ccaaggcccg gcagatcatg gcggacctgg acgcggtcgg
     1981 gaacaccacc aaggccatca cgaagggagt ggccatagga tccgcggtca ttgcggccgt
     2041 gtccctgttc ggctcgttcc ttacggacgt ctccaaggtg caggcccaga tgggaatcct
     2101 ggagaacctg cgaatgatct cgacgggaat ccgggtttcg gtgccctccg tgtttatcgg
     2161 gatgctgatc ggcggtgccg tgccctggct gttctcctcc cttaccatca acgcggtcgc
     2221 tcgcgccgcc tctttgatcg ttgtcgaagt gcgtcggcag ttccgcctcc ccggcctcat
     2281 ggagggaaag atccagcccg actacgccca ggcggtggga atctgcaccg cggcggctca
     2341 gcgggagctc atcggtcttt cgctgatctc catcctgacc ccgctgatcg tcggcatgct
     2401 tcttggggtc gaagcgttgg gcggcttcct ggccggcgcc attctctcgg gtcagctcct
     2461 ggcggtgttc atgtctacgg cgggcggcgc gtgggacaac gccaagaaga ccatcgagga
     2521 cggcctgtac ggcggcaagg gctccgaggc gcacaaggct tccgtggtcg gtgacacggt
     2581 gggtgatccc ttgaaggaca cctcgggccc cgccctgaac cccatgttga aggtcatcaa
     2641 cctggtctcc gttctggcgg ctcccatcct catcaagttc acgggtgcca gcgtgggcct
     2701 cgtggttgcc ctggtggtct gcgtcatcgt ggtcttcggc gccgttgtgt tctccaagcg
     2761 gggaaccctc aacctccgca agagaagcgc ggacgccggc ttctacgccg aggggtcttg
     2821 acggagaaag cccccctctt catgctgagg gccgcctgcc agcgcccccg ttcgccgggc
     2881 ctttgggtgg tacgccaccc cctcgggtcc cgcggacggg ggcgctttgc gccatccctg
     2941 gctagcgccc cctcggcgtc ctgcctacgg gggcgcggcg cgtagaagga cggatgggca
     3001 aactagggac tctctctcgg cgggtcaaag aggtggtgat cgggaaggcc cgatccgtcg
     3061 aggaccctga gc
//
LOCUS       DASRZS010000244         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4370612, whole genome shotgun sequence.
ACCESSION   DASRZS010000244 DASRZS010000000
VERSION     DASRZS010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4370612"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1089
                     /locus_tag="VFH83_06110"
     CDS             31..1089
                     /locus_tag="VFH83_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HET6485972.1"
                     /translation="MTGELSDELHEVPLDRENQDLKEILEIAFQITAQLEIDNVIKNV
                     VWSLVSKFQLETVTVLLPGDDDEDHVQIVSYRGLKPEVLPFHLPSILPVLSFMEKDEY
                     SQIQFSYFRENFSDQTMVDALARLEPDVLVPLRTDKGAIGVILLPRFGPDAEYGLLEL
                     QYITRIARFAAIAIENASLFRQATTDRMTGLFSHHFFEKTLEEELERARRYKTTFSLV
                     MFDIDHFKAFNDAYGHLQGDRIIREIARLITKSIRQVDFPARYGGEEFAVILPAVDIR
                     GALVVAERLRKRVEQYAFASSEGGDPLHVTISLGVTEFDPESAYAPTEVIREADRALY
                     RSKELGRNRVSTSTPRPG"
     gene            1109..>2514
                     /gene="aroF"
                     /locus_tag="VFH83_06115"
     CDS             1109..>2514
                     /gene="aroF"
                     /locus_tag="VFH83_06115"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET6485973.1"
                     /translation="MIVVLERTIRDQDRARIRDFLVNKGYTIREIVGEEETILGAVGI
                     KSVDVREVELLPGVARVIPITSPYKLASREFRVEDTIISFGDPAHPVRLGGNRIVTIA
                     GPCAVESRQQVLECAAAVRQSGAVLLRGGAWKPRTSPYAFQGLGEEGLAYLKEAGEAT
                     GMPIVSEIVSPEHVEVMSDYVDVLQVGARNMQNFELLKKVGALGKPVLLKRGLAATIQ
                     DLLMSAEYLLAAGTDKVILCERGIRTFETATRNTLDLSAIPVVKKLSHLPILVDPSHG
                     TGERAKVSPMALAAVAAGADGITVEVHTDPENALSDGPQSLYPEQFEKLMRDIEALAP
                     VLGKELARLPMKADRTAGKPMAVLAPAQPGSATLVAFQGERGAFGEQAIRQWFASEVT
                     SLPVSEFRSVFDAVLDGSARYGVVPIENSLTGSIHQNYDLLLQYPDVRIVGEQKVRIV
                     HNLIALPGSSMEKIRRVYS"
BASE COUNT          435 a          852 c          811 g          416 t
ORIGIN      
        1 ccgccaatcg ggctatactg gaagccacct atgaccggcg aactgtccga tgagctgcac
       61 gaggtgccgc tggatcggga gaaccaggac ctgaaagaga tcctggagat cgccttccag
      121 atcacggctc agctcgagat cgacaacgtg atcaagaacg tcgtatggag cctcgtgtcc
      181 aagttccagc tcgagacggt gacggtgctc ctgcccgggg acgacgatga ggaccacgtc
      241 cagatcgtct cctatcgcgg gctgaagccc gaggtcctgc cgttccatct cccgtccatc
      301 ctcccggtgc tgtccttcat ggagaaggac gagtacagcc agatccagtt ctcctacttc
      361 cgcgagaact tctccgacca gaccatggtg gacgcgctgg cccgcctgga accggacgtc
      421 ctggtgcctc tgcggacgga caagggagcc atcggcgtca tcctgcttcc gcgcttcggc
      481 ccggacgccg agtacggtct cctggagctg cagtacatca cgcggatcgc gcggtttgcc
      541 gccatcgcca tcgagaacgc cagcctgttc cgtcaggcca ccacggaccg catgacgggt
      601 ctcttctccc atcacttctt cgagaagacc ctggaggagg agctggagcg ggcgcgccgt
      661 tacaagacca ccttcagcct ggtcatgttc gacatcgacc acttcaaggc gttcaacgac
      721 gcctacggcc acctccaggg ggaccgcatc atccgagaga tcgcccgtct catcaccaag
      781 tccatccgac aggtggactt ccccgcccgt tacggcgggg aggagttcgc cgtcatcctg
      841 cctgcggtgg acatccgggg cgcgctcgtg gtggcggagc gcctccgcaa gcgggtggag
      901 cagtacgcct ttgccagctc cgagggcggg gatccgctcc atgtgaccat ctccctgggg
      961 gtgaccgagt tcgacccgga gtctgcctac gctcccacgg aggtgatccg ggaagccgac
     1021 cgcgcgctct accggtccaa agagctcggg agaaaccggg tgagcacctc cacgccgcga
     1081 cccggctgag agcggatcgg agccttccgt gatcgttgtc ctcgagcgga ccatccggga
     1141 tcaggatcga gcccgcatcc gcgacttcct ggtgaacaag ggctatacca tccgagagat
     1201 cgtcggcgag gaggagacca tcctcggcgc cgtagggatc aagtccgtgg acgtgcggga
     1261 ggtggagctg cttcccggcg tcgctcgcgt catccccatc acctcgccgt acaagctcgc
     1321 ctcccgcgag ttccgcgtgg aggacaccat catctccttc ggagaccccg cccaccccgt
     1381 gcggctcggc ggcaaccgca tcgtgaccat cgccggcccg tgcgcggtgg agtcccgaca
     1441 gcaggtcctg gagtgcgccg ccgcggtccg ccagtccggc gcggtgctcc tgaggggagg
     1501 cgcctggaag ccgcgcacct cgccttacgc cttccagggg ctgggagagg aaggcctggc
     1561 gtacctgaag gaggcggggg aggcgaccgg gatgcccatc gtctccgaga tcgtctcacc
     1621 cgagcacgtc gaggtgatga gcgactacgt ggacgttctg caggtcggcg cgcggaacat
     1681 gcagaacttc gagctcctca agaaggtggg cgcgctgggc aagccggtgc ttctgaagcg
     1741 cggtctggcc gcgaccatcc aggacctcct catgtccgcg gaatacctgc tggcggcggg
     1801 gacggacaag gtcatcctct gcgagcgcgg catccgcacc tttgagacgg caacccgcaa
     1861 caccctggac ctctcggcca tccccgtggt gaagaagctg agccaccttc ccatcctggt
     1921 ggatcccagc cacggcaccg gagagcgcgc caaggtcagc cccatggccc ttgccgccgt
     1981 ggcggccggc gcggacggca tcacggtgga agtgcacacg gaccccgaga acgcgctctc
     2041 cgacggcccg cagtcgctgt atcccgagca gttcgaaaag ctcatgcgcg atatcgaggc
     2101 gctggcgccg gtgctcggca aggagctggc ccggttgccg atgaaggcgg atcggacggc
     2161 ggggaaaccg atggcggtcc ttgctcccgc ccagcctggc tccgctaccc tggtggcgtt
     2221 ccagggggag cggggcgcgt tcggcgagca ggccatccgc cagtggtttg cctcggaggt
     2281 gaccagcctg cccgtgtcgg agttccgctc cgtgttcgac gccgtgctgg acgggagcgc
     2341 gcgctacggc gtggtgccca tcgagaactc cctcacgggc tccatccacc agaactacga
     2401 cctcctgctc caatatcccg acgtgcggat cgtgggtgag cagaaggtcc gcatcgtgca
     2461 caacctcatc gccctgcctg gctcgtctat ggaaaagatc cggcgcgtgt actc
//
LOCUS       DASRZS010000245         8666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_909229, whole genome shotgun sequence.
ACCESSION   DASRZS010000245 DASRZS010000000
VERSION     DASRZS010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8666
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909229"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1004
                     /locus_tag="VFH83_06120"
     CDS             39..1004
                     /locus_tag="VFH83_06120"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6485974.1"
                     /translation="MSGETTVAGVASWASAVRESVRVAFRGSPQTVDRLLVALLCRGH
                     VLIEDVPGVGKTVLARALALSVGGIFRQVQCTPDLLPADVLGVSLYNQATGAFEFRDG
                     PIMTNVLLVDEINRATPRTQSAFLEAMAEGQVSVEGRSIALPDPFLLVATESPVESEG
                     TFPLPEVQKDRFFLCLHLGYPSREDELAVMVQQRRPRSPLEEVRPAAGIEELREMQRR
                     VLSVHVSDHLRAYILALVSATRSDPRLVLGVSPRGSLALYKGGQALAALRGRSFVTPE
                     DIRDMALPVLQKRLILKSEPAGRGLTEENVVQELLSRTPIPPLED"
     gene            1159..1962
                     /locus_tag="VFH83_06125"
     CDS             1159..1962
                     /locus_tag="VFH83_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6485975.1"
                     /translation="MTARERSNPGYRQLQAQLEQAEALIRRLRDVKKDALGPKGQSAL
                     TRLSAVAAHLERANNALRQTVKSVGDGLKPAARGTVKRSSTVRATSGRAAPGRTSTRQ
                     APKRGSSEAERKIRVIIADDHRIFREVLTRVLNLCSDIEVIGEAEDGEVAVTLSTRLK
                     PDVVLMDASLPGLSGVEATRRILTTTSASRIVGLSARDDGDAAMLMVQAGAVAYMTKG
                     GQTSQLLDAIRFAAGRPGRLPAGQKAPDIRVDGAPGAGLSEAAASTTAK"
     gene            complement(1928..2758)
                     /locus_tag="VFH83_06130"
     CDS             complement(1928..2758)
                     /locus_tag="VFH83_06130"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6485976.1"
                     /translation="MKHEEHSWKTSDGIEIFAQRWAPDGPPRAAVALVHGLGEHSTRY
                     AHVAERLTSDGFALTAFDHRGHGRSGGPRVFAPSYEAVMGDIDHHLEDCRSRFPKVPV
                     FLYGHSWGGALVLYHSYKRNAGLAGVVASSPGLQSGIPQPALKIAAGKVLSRIAPTLR
                     IPTGFAFEGLTRDKQLLDAAAKDPLMKSGISARLGIDILAAGEWIRGHDTFPYPLLLM
                     QGTADRYVDPATNIEFARRLNGTVTLKTWEGCLHELQNETVRDQVIEFISRWLKQQLR
                     "
     gene            2901..3965
                     /locus_tag="VFH83_06135"
     CDS             2901..3965
                     /locus_tag="VFH83_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485977.1"
                     /translation="MRRMTNRQRLLAVLHGRELDRVPFIQYDDCAAPNNEIWPVLGRN
                     AIGRLRWIDACATATPSCRWSVEEFSREGLRGRRTTLRAPRGTLVEEALLEPTYGAAS
                     IKKHFVSGKDDWEVLLSCLKDVVVIADAERLAAADREIADDGLPHVAVQRTPYQQLWI
                     QWVSLEDLVPALVDWPDITAAVIAELIRIQREIFRAVTRLPVPYIVFPDNITAPPIGT
                     RYFRELCVPLYDELGEMLADRDVPVFVHMDGDLKPLWPAIGDSRVQGLDSLSPPPDND
                     TSTADAVRNWPRMKVFVNFPSSVHVQDPDRVYRVAQEILEQAGHTGRLSIQISENVPP
                     GVWRRSFPAIVRAIDEFGRP"
     gene            4080..5327
                     /locus_tag="VFH83_06140"
     CDS             4080..5327
                     /locus_tag="VFH83_06140"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET6485978.1"
                     /translation="MAELNPFKIAQKQLDAAAATLGLDEATHQLLRWPQRELQVSFPV
                     RMDDGSMRIFHGYRVQYNGARGPTKGGLRWHPDETIDTVRALSAWMTWKTAVVDLPLG
                     GGKGGVTCNPKELSAGEKERLARAYIRAVGRFLSDTQDVPAPDVYTSPDIMAWMMDEY
                     ETIVGRHCPGVITGKPIALGGSAGRGDATARGGIYVTREAAARLQLDLKGKAMAIQGF
                     GNAGQHAALLGSELLGLKLVAASDSQGGVYNARGLDVKALVDYKQRVGSLAGFTAAEP
                     ITNEKLLELDVTVLFPAALENVITDANAGRVKARIVCELANGPTTPEADVVLAKNNLF
                     VIPDFLANAGGVTVSYFEQVQNAYNYYWNLDLVYSRLDEKMTKAFHDVYEMKQRHQVN
                     MRDAAYLVSVSRVAEACKLRGWV"
     gene            5324..7633
                     /locus_tag="VFH83_06145"
     CDS             5324..7633
                     /locus_tag="VFH83_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET6485979.1"
                     /translation="MTTGGPPGGLSVVRGYNLFRTISRYPKLLPQMRAAFLEVLIERG
                     AVSREGLAQQVTQQLAADGVADTESARADYTDALIDLLFANTLSAEEAENWINLIRKR
                     DLCQELGRVVAAEHSDSAAIWQALRAFCDIPKGELYLAREEAEGIRVSLLAYYISSQL
                     PFIGIAKNHVTIRDIEAVLGHTLGHVRYPGKLGGKAAGMIVAEKILLPILEHRDPEFE
                     RYIATPETWYISSGVFSDFIDRNHFYLFHTHKYRTREAIDDQYQQIGSLFEAASFPPD
                     VVEEFRSLIAQIGEHPIIVRSSSYLEDNFGYAFSGKYQSIFLANQGSEEERLVAFIQG
                     VKTVLASMYGPDPIIYRRDHGLLDYNERMAMIVQKVVGRRFGDWFFPFAAGVMYSFNS
                     WRWSPRIRKTDGLVRLVFGLGTRAVDRVGGDYPRMIPLSHPLLRPEVGAEQVCKYSQR
                     MVDVINLAKGTFETVDFRTLASATRHPDLHAAVSVVEDGEVRAPQFRLQELPPASLCL
                     TFDNLLTRSSFVHLVKKVLSTVQKAYGRPVDIEFAWDGEKLYILQCRSLSMRREVERV
                     TIPADVPEEDRLFLTHAGLSSAVITDIEYLVYVDPRAYDRLATVEAKQRVGWAVGQIN
                     KRLEGKRFVLLGPGRWGSNDINLGVRVTYADINKTRLLVEIAFARDGYTPEVSYGTHF
                     FQDLVEADIAILPLYPDASGNELNEGLLLGSPNALASLDSSLGDLCGVIHVIHVPTVR
                     PGQLLHVYLDGESQEGVGRFGPAVEAKLP"
     gene            complement(7652..>8666)
                     /locus_tag="VFH83_06150"
     CDS             complement(7652..>8666)
                     /locus_tag="VFH83_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485980.1"
                     /translation="PDWLGFSPWGQTLDRWRTESGIADLSVPRYFGLDPGFVVPKLEM
                     GPLPHFEERVVSEDPEFITAIDYRGILRRDRRDGASMPEWIGHPVKGPEDWARYKAER
                     LQGPGTRVPDLTAFRCAAERDGACVQLGEFPWGVFGTLRDIVGAEECLLAFYDYPEMV
                     QDIIDTNVQLWLRVYEEAVGQIRVDHIHIWEDMSGRQGSLISMEMLERFMMPSYDRIV
                     AFARSHGIPLVSVDTDGQVAELVPAMMRHGINVFMPFEVQAGNDVLRYRGLYPGLGIM
                     GGLDKSALAKGKAEIHRELDRAEKMLARGGWVPGFDHLIPPDVPWASFEYAVKELRRL
                     VFG"
BASE COUNT         1444 a         2919 c         2917 g         1386 t
ORIGIN      
        1 taccgggagc tgtcgcgcat ggtcgacgga gtcgtggcat gagcggagag acgacggtcg
       61 ccggcgtggc ctcgtgggca tccgcggtcc gcgaatcggt ccgggtcgcg ttccggggat
      121 ctcctcagac ggtggatcgg ctcctggtgg ctctcttgtg tcgcgggcac gtgttgatcg
      181 aggacgtgcc gggcgtcggg aagacggtgc tggcccgggc gctcgccctg agcgtcggcg
      241 ggatcttccg tcaggtccag tgcacgccgg acctgcttcc cgccgacgtg ctgggagtct
      301 cgctctacaa tcaggcaacg ggcgcctttg agttccgcga cggaccgatc atgaccaacg
      361 tgctgctggt cgacgagatc aatcgcgcca cgcccaggac ccagtccgcg tttctggaag
      421 ccatggccga gggccaggtc agcgtggaag gccggagtat cgccttgccg gatcccttcc
      481 tgctggttgc caccgagagc ccggtcgaga gcgaggggac cttcccgctg cccgaggtcc
      541 agaaggatcg cttcttcctc tgcctgcacc tggggtaccc aagccgggag gacgagctgg
      601 ccgtgatggt gcagcagcgc aggccgcgaa gccccctgga ggaggtccgt cccgccgccg
      661 gcatcgagga gcttcgggag atgcagcggc gggtgctctc ggtgcacgtg agcgaccacc
      721 tgcgcgccta catcctggcg ctggtgagcg ccactcggtc cgaccctcgg ctggtccttg
      781 gcgtctcccc gcgcggctcc ctggcgctct acaaaggcgg tcaggccctc gctgctctgc
      841 gtggaaggag cttcgtgacg ccggaggata tccgggacat ggcgctcccg gtgttgcaga
      901 agaggctgat cctcaaatcc gagcccgccg ggcgcggtct caccgaggag aacgtggtcc
      961 aggagcttct ctccaggacc cccatcccgc ccctggagga ctaaggggaa ccccgacccc
     1021 atggtcccgg cctccgcctg ggccgcccgg cgccacggcc atcccgccgc ctggtttttt
     1081 ctcgtcggcc ttgccagcgc ctttgccgca tgcgataata atggtgggaa ctcgttggca
     1141 ggcaaggaga atggtgatat gacggcacgg gagcggagca atccgggcta ccggcagctg
     1201 caagcgcagc tcgagcaggc ggaggcactc atccgccggc tgcgagacgt caagaaggat
     1261 gctcttggcc ccaaggggca atcggcgctc acccggctat ccgccgtggc ggcgcacctg
     1321 gagcgagcca acaatgccct gcgccagacc gtgaagagcg ttggggacgg cctcaagccc
     1381 gccgcccgcg gcacggtcaa gcgctcgtcg accgtaaggg ccacgtcagg ccgggccgcg
     1441 cccggccgca cctcgactcg ccaagcccca aagcgcgggt ccagcgaagc cgaacggaag
     1501 atccgggtca tcatcgcgga cgatcatcgg atcttccgcg aggtcctgac gcgggtcctc
     1561 aacctgtgct ccgatatcga agtgatcgga gaggcggaag acggggaggt ggccgtcacg
     1621 ctctccacac ggctgaagcc ggacgtggtc ctgatggatg cgagcctgcc gggcctgagc
     1681 ggcgttgagg ccacccgacg gatcctgacc accaccagcg cctcccgcat cgtgggcctc
     1741 tcagcgcggg acgatgggga cgctgcgatg ctcatggtgc aggccggggc cgtggcctat
     1801 atgaccaagg gcgggcagac ctcccagctc ctggacgcca tccgcttcgc ggcggggcgg
     1861 cccggcaggc tgcctgccgg tcagaaggcg cccgacattc gagtggatgg ggctccgggg
     1921 gcggggctca gcgaagctgc tgcttcaacc accgcgaaat aaactcgatt acctgatcgc
     1981 gcacggtctc gttttgcagc tcgtgcaggc atccctccca tgtcttgagg gtgaccgtcc
     2041 cgttgagacg cctcgcgaac tcgatgtttg ttgcggggtc cacgtagcgg tccgcggttc
     2101 cctgcatgag gaggagcgga tacgggaagg tgtcgtgacc gcggatccac tctcccgcgg
     2161 ccaggatatc gatgcccagt cgcgcggaga tcccggattt catcagggga tccttggccg
     2221 ccgcgtccag gagctgcttg tcgcgggtca gcccctcgaa cgcgaaaccg gtgggaatcc
     2281 ggagggtcgg tgcgatgcgg gagagcacct taccggccgc aatcttcagc gcgggctgtg
     2341 gtatccctga ctggaggccg gggctggacg cgacaacgcc ggcgagcccc gcgttccgct
     2401 tgtaagaatg gtaaagcacc agcgctcctc cccagctatg gccgtagaga aagaccggta
     2461 ccttggggaa gcggctgcgg caatcctcca ggtgatggtc gatgtcgccc atgaccgctt
     2521 cgtaggacgg ggcaaacacc ctgggaccgc cggaccgtcc atgcccgcga tggtcgaagg
     2581 cggtgagcgc gaacccatcc gacgtcagcc gctcggccac gtgggcgtag cgggtgctgt
     2641 gctcgcccag gccatggacc agcgccaccg ccgctcgcgg cggcccgtcc ggcgcccacc
     2701 gctgggcgaa gatctcaatg ccgtcggacg tcttccagga atgctcctcg tgcttcatgc
     2761 tcgctcctgc gcgtactccc aacggtactg gcggcgatgg ggcggcggca agctcgccgc
     2821 cggaatgcag gctcgcagcc gggcggcggg ctcgccgccg gactgttgac cgggcgcggg
     2881 gccaacggaa tactcgtgcc atgaggcgga tgaccaaccg acagaggctg ctcgccgtgc
     2941 tgcacggccg ggagctggac cgggtgccct tcatccagta tgacgactgc gcggcgccga
     3001 acaacgagat ctggcccgtg ctcggccgca acgccatcgg caggctgcgc tggatcgacg
     3061 cgtgcgccac ggcgaccccg tcctgtcggt ggagcgtgga ggagttcagc cgggaaggcc
     3121 tgcgtggccg ccgcaccacc cttcgagcgc cgcggggcac gctggtggag gaggcgctcc
     3181 tggagcccac gtacggcgcc gcctccatca agaagcattt cgtctcggga aaggacgact
     3241 gggaggtcct gctctcctgc ctgaaagacg tggtggtgat cgccgacgcg gagcgcctgg
     3301 cggccgcgga ccgcgagatc gcggatgacg gcctcccgca cgtcgccgtg cagcgcaccc
     3361 cctatcagca gctctggatc cagtgggttt ccctggagga cctggtcccc gccctggtgg
     3421 actggcccga catcaccgcc gccgtgatcg cggagctgat ccggatccag cgggagatct
     3481 tccgcgccgt cacgcggctg ccggtcccct acatcgtgtt tccggacaac atcacggcgc
     3541 cgcccatcgg aacacggtac ttccgagagc tctgtgtgcc gctctacgac gagctgggag
     3601 agatgctcgc ggaccgggac gtgcccgtct ttgtgcacat ggacggtgac ctcaagccgc
     3661 tgtggcctgc catcggagac tcccgggtcc agggcctgga ttccctctca ccgccgccgg
     3721 acaacgacac cagcacggcg gatgcggtcc gcaactggcc ccgcatgaag gtgttcgtca
     3781 acttcccgtc gtcggtccac gtgcaggatc ccgaccgggt ctaccgcgtc gcccaggaga
     3841 tcctcgagca ggccgggcac acgggtcggc tgtccatcca gatctcggag aacgtgccgc
     3901 ccggagtctg gaggaggagc ttccccgcga tcgtccgcgc catcgacgag ttcggaaggc
     3961 cctgaaccgc ggaatctctt gatgcctcca gccgggcgga ctatagtgga agctggtacc
     4021 gcgcccggat gctggatcgc aggcaccccg acacggcgcg gctcaaggag taacacccaa
     4081 tggctgaact gaaccccttc aagatcgcgc agaaacagct cgacgcggcc gccgccacgc
     4141 tcggactgga tgaggcgact caccagctcc tgcgctggcc gcagcgggag ctccaggtga
     4201 gcttcccggt ccgcatggac gacgggtcga tgcgcatctt ccacggctac cgcgtgcagt
     4261 acaacggggc ccgcgggccc accaagggcg gtctccgctg gcatcccgac gagacgatcg
     4321 acacggtccg cgcgctctcc gcgtggatga cctggaagac ggcggtcgtc gaccttccgt
     4381 tgggcggggg gaagggtggg gtcacctgca atcccaagga gctctccgcg ggggagaagg
     4441 agcgcctggc gcgcgcctac atccgcgcgg tgggccggtt cctttcggat acccaggacg
     4501 tcccggcgcc ggatgtctat acgtcccccg acatcatggc gtggatgatg gacgagtacg
     4561 agaccatcgt gggtcggcat tgtcctgggg tcatcaccgg gaagcccatt gcgctgggcg
     4621 gatccgccgg acggggagac gccaccgcgc gcggcggcat ctatgtgacg cgagaggcgg
     4681 cggcaaggct ccagctcgat ctcaagggca aggccatggc catccaggga ttcgggaacg
     4741 ccggccagca cgccgcgctc ctgggatccg agctcctggg gctgaagctc gtcgcggcat
     4801 cggactccca gggaggggtc tacaacgctc gcgggctgga cgtgaaggcc ctggtggact
     4861 acaagcagcg ggtcggcagc ctggccggtt tcaccgccgc cgagcccatc accaacgaga
     4921 agctcctgga gctggacgta accgtgctct tccccgcggc cctggagaac gtgatcacgg
     4981 acgccaacgc gggccgcgtg aaggccagga tcgtgtgcga gctggccaac ggccccacca
     5041 ccccggaagc cgacgtcgtg ctggccaaga acaacctgtt cgtcatcccc gatttcctgg
     5101 cgaacgccgg cggggtcacc gtctcctact tcgagcaggt tcagaatgcc tacaactact
     5161 actggaacct ggacctggtc tacagccgcc tggacgagaa gatgaccaag gcgttccacg
     5221 acgtgtacga gatgaagcag cgccaccagg tgaacatgcg ggacgccgcg tacctggtgt
     5281 ccgtgagccg ggtcgcggag gcctgcaagc ttcgcgggtg ggtgtgacca cgggcgggcc
     5341 ccccggcggt ctcagcgtcg tccggggcta caacctcttt cggaccatct cgcgctatcc
     5401 caagctgctg ccccagatgc gggcggcgtt cctggaggtc ctgatcgagc gcggggcggt
     5461 gagccgggag ggcctcgccc agcaggtgac ccagcagctc gcggcggacg gcgtcgcgga
     5521 caccgaatcc gctcgcgcgg actacacgga cgcgctgatc gacctgctct tcgccaacac
     5581 cctgagcgcg gaggaggcgg agaactggat caacctgatc cgaaagcgcg acctctgcca
     5641 ggagctgggc cgggtggtgg ccgcggagca ctcggattcc gcggccatct ggcaggcgct
     5701 ccgcgccttc tgcgacattc ccaagggcga gctgtacctg gcgcgggagg aggcggaagg
     5761 catccgcgtc tccctcctcg cctactacat ctccagccag ctccccttca tcggcatcgc
     5821 gaagaaccac gtgaccattc gggacatcga ggccgtgctg ggccacaccc tcgggcacgt
     5881 gcgctatccc ggcaagctgg gcggcaaggc cgcggggatg atcgtggcgg agaagatcct
     5941 cctccccatc ctggagcacc gggaccccga gttcgagcgc tacatcgcga cccccgagac
     6001 ctggtacatc agctccggag tgttctccga tttcatcgac cgcaaccact tctatctgtt
     6061 ccatacccac aagtaccgca cccgggaggc cattgacgac cagtaccagc agatcgggag
     6121 tctcttcgag gcggcctcct tcccgccgga cgtcgtggag gagttccgat cgctgatcgc
     6181 ccagatcggg gagcatccca tcatcgtgcg gtcctccagc tacctggagg acaacttcgg
     6241 gtacgcgttc tccgggaagt atcaatccat cttcctggcc aaccagggct cggaggagga
     6301 gcgcctggtc gcgttcatcc agggggtgaa gaccgtcctg gcgagcatgt acggaccgga
     6361 ccccatcatc taccgccggg accacggcct gctggattac aacgagcgga tggccatgat
     6421 cgtgcagaag gtggtgggaa ggcgcttcgg cgactggttc ttccccttcg cggccggggt
     6481 gatgtactcc ttcaactcct ggcggtggag cccgcggatc cgcaagacgg acggactggt
     6541 gaggctggtc ttcgggctgg gcacccgcgc ggtggaccgg gtgggcgggg actacccccg
     6601 gatgatcccg ttgagccatc cgctcctgcg gcccgaggtg ggcgcggagc aggtctgcaa
     6661 gtactcgcag cggatggtgg acgtcatcaa cctggccaag ggcacctttg agaccgtcga
     6721 cttccggacg ctggcctcag ccacccggca cccggacctg cacgcggcgg tctccgtggt
     6781 agaggacggc gaggtgcgcg ctccgcagtt ccggctccag gagctgccgc cggcctctct
     6841 ctgcctcacg ttcgacaacc tgttgacgcg atcctcgttc gtgcacctgg tgaaaaaggt
     6901 gctgtcaacg gtccagaagg cgtatggcag gccggtggac atcgagttcg cctgggacgg
     6961 cgagaagctg tacatcctgc aatgccgatc gctctccatg cggcgggagg tggagcgcgt
     7021 gaccataccg gccgacgtgc cggaagagga tcggctgttc ctgacgcacg ccgggctttc
     7081 cagcgccgtg atcacggaca tcgagtacct ggtgtacgtg gatcctcgcg catacgaccg
     7141 gctggctacc gtggaggcca agcagcgcgt gggctgggcg gtcggccaga tcaacaagcg
     7201 tctggaaggg aagcgcttcg ttctcctggg ccccggacgc tggggcagca acgacatcaa
     7261 cctgggcgtg cgggtgacct acgcggacat caacaagacc cggctcctgg tggagatcgc
     7321 tttcgcgcga gacggctaca cccccgaggt ctcctacggc acgcacttct tccaggacct
     7381 ggtggaggcg gatatcgcga tccttcccct gtatccggat gcgtcgggca acgagctgaa
     7441 cgagggcctg ctcctgggat cccccaatgc gctcgcttcc ctggattcca gcctgggaga
     7501 cctgtgcggg gtgatccacg tcatccacgt gcccacggtc cgccccggtc agctcctcca
     7561 cgtctacctg gatggcgagt cccaggaagg ggtgggtcgg ttcgggcccg cggtggaggc
     7621 gaagcttccc tgagcgcgcg gaaggggccg gtcagccgaa caccagccga cgcagctcct
     7681 tcaccgcgta ctcgaagctg gcccacggca cgtcgggcgg gatcaggtga tcgaagcccg
     7741 gtacccagcc gccccgagcc agcatcttct ccgcgcggtc cagctcccgg tggatctccg
     7801 ccttcccctt ggccaacgcg ctcttgtcca acccgcccat gatgccgagg ccgggataga
     7861 gcccgcggta gcggagcacg tcgttgcccg cctggacctc gaacggcatg aagacgttga
     7921 tcccgtggcg catcatggcc ggcaccagct cggccacctg gccgtccgtg tccacggaaa
     7981 ccagcggtat gccatgggag cgcgcgaagg ccacgatccg gtcgtaggag ggcatcatga
     8041 accgctccag catctccatg gagatcagcg aaccctggcg gcccgacatg tcctcccaga
     8101 tgtggatgtg gtcgacccgt atctgcccga ccgcctcctc gtagacgcgc agccagagct
     8161 ggacgttggt gtcgatgatg tcctgcacca tctcggggta gtcgtagaag gccaggagac
     8221 actcctcggc gcccacgatg tccctcaggg tgccgaagac gccccacggg aactctccaa
     8281 gctgaacgca ggccccgtcc cgctcggccg cgcaacgaaa ggccgtgagg tccgggaccc
     8341 tcgttcccgg cccctggagc ctctccgcct tgtaccgagc ccagtcctcc ggccccttca
     8401 ccggatggcc gatccactcc ggcattgacg ccccgtcccg gcgatcccga cggaggatcc
     8461 cgcggtagtc gatggccgtg atgaactccg ggtcctcgct aaccaccctc tcctcgaagt
     8521 ggggcagggg acccatctcg agcttcggga cgacgaagcc gggatccagg ccaaagtacc
     8581 gggggaccga gagatcggcg atccccgatt ccgttcgcca gcgatcgagc gtctgtcccc
     8641 agggggagaa accgagccag tcggga
//
LOCUS       DASRZS010000246         5608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_525684, whole genome shotgun sequence.
ACCESSION   DASRZS010000246 DASRZS010000000
VERSION     DASRZS010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525684"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VFH83_06155"
     CDS             <1..914
                     /locus_tag="VFH83_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6485981.1"
                     /translation="KGTGHVLGIEIQTERYSAVALDLEGRIVQSRSIPFSWDGRHLST
                     VFLEIVSEATQALDISGDGLLGIGVGLSGIVDSSTGTIRQSLPLGVTSPLDFYSSSAR
                     LIGKPLCIDNDANCCCWAQLVRGRDARPREFLFILGEFRKERAGDLGGGGLSVGLGLV
                     LGGRVHLGASSSAGEFRSVFRTSETGSQFSVEPAVMERAENDPSAFERVARELGRNVA
                     LLVNTLDLSHVFIGGNIERRQRELVPILEKEIESNWPYGRKSLSIEASELGELVVAYG
                     AAAMFLERLFSLPQIQEHSVPVGVSAG"
     gene            complement(921..1217)
                     /locus_tag="VFH83_06160"
     CDS             complement(921..1217)
                     /locus_tag="VFH83_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HET6485982.1"
                     /translation="MRDFRAFMTYGKFDDVVIQMALREIDAPTLAVALADQDETVQEI
                     VLRNMSQRAARCLSEDIRQNASAPQGPDRESIQDAQRYFAGLVEWADRQYARTG"
     gene            complement(1277..2467)
                     /locus_tag="VFH83_06165"
     CDS             complement(1277..2467)
                     /locus_tag="VFH83_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HET6485983.1"
                     /translation="MDESSARARSGLDYLRQRGREGAGERPSASLRRPEPPSVSPDRR
                     FPGWVHESEFVYRRRQTFPGLVVPSWPSRISPEAVSPGGLLFFDTETTGLSGGAGTMI
                     FLFGVGWSEGPDFVVEQLFLSDFPGEPEFLLGIKQLLPSDRALVSYNGRSFDAHLLRS
                     RFLMNRIDEESWLHVDLLYHARRLWKSVSSDCSLRGIESAILGVDRGDDVPGMEIPGI
                     WYDFLRTGRPDRLSLVFDHNVTDITSLARIYGTLGRLLGGELAAAPVDQGALGRWLLG
                     PDPESGIAILQKAYEDGDSRAGLSLGLHFKREGRWEEALRVWQSAVERSHSLLASVEL
                     AKHLEHRARRPDLALQVIERLLGWGLPLGKQDRTAIAHRLQRLRRKVEGRTASPAEGP
                     EEPS"
     gene            complement(2467..4764)
                     /locus_tag="VFH83_06170"
     CDS             complement(2467..4764)
                     /locus_tag="VFH83_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6485984.1"
                     /translation="MLADMIGSLKRDREFMDRVTHWEVVPAQPGRYVDIPAGVDSRIR
                     AALASRGITRIYSHQAETWEAVRQGKSVVIVSPTASGKTLAYNLPVLQSILEDPDTRA
                     LYLFPTKALSQDQQSELNEVVLGGEIPVKVFTYDGDTPTSIRISARDEGRIVITNPDM
                     LHTGILPNHTKWIKALRHLRFVVIDEMHSYRGVFGSHMTSVIRRLKRIAAFYGAKPQF
                     ICCSATIGNPHELARRILEEDVQLIDRNGAPSGERHFVLYNPPLVDRVQGIRRSVVLE
                     SQRLATRLLKAGVKTIVFARSRIRTELIATYIRDSLRNFYTDDGGIRVESYRGGYLPN
                     ERRAIEKGLRDGSIQGVVSTNALELGIDIGGLDASIIAGFPGTISSTWQQAGRSGRRA
                     TVSLSILVASSSATDQFVIQHPEYFFSRSPECGYIDPGNVFIQTDQLKCAVFELPFED
                     SEGFGEGTADLLGYLSEQGVVRHAASKWYWSDQAYPAENVSLRTSTPENVIIVDTTRG
                     RDEVIGEMDLSSAKLLIFDHAIYLHLGEQFMVTKLDIANRRCFVETTDTDYWTDSIMK
                     SDIKVLSQDDQREVAGIPVVSGDILVRTQATKFKKLKYHTNENIGYGDINLPADEMHT
                     RAAMLLFDEVSRPGRAFAAMNETDQGVVIQRLGVLLRTVSPVFLLCDPRDLGVSERVR
                     DPHFERPCLYVFDQYPGGIGLSEGFIRQLPQIVRGAREVVDRCPCARGCPSCIGAPED
                     EPAESSDPKAAVRGFLRDWALALGE"
     gene            4865..>5608
                     /locus_tag="VFH83_06175"
     CDS             4865..>5608
                     /locus_tag="VFH83_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET6485985.1"
                     /translation="MDKIVSIVLAGGKGQRLHPLTAKRSKPAVPFGGKFRLVDIPISN
                     CMHAGFREIYILTQYNSASLHRHINNTYRFDAFGRGFVEVLAAVQTPESEEWYQGTAD
                     AVRQNFLHFADHSPTHYLILSGDQLYRMDLHAFFDAHVQSKSDVTVACLPVTKEKASS
                     LGILQLDERYHITRFVEKPPADSDMSSLAVPRRIREREELDIEGDAYLASMGIYVFSA
                     PALAKALDNDLTDFGSQVIPASIEKMKVSG"
BASE COUNT          937 a         1856 c         1859 g          956 t
ORIGIN      
        1 acaagggcac cggtcacgtc ctgggcatcg agatccagac cgagcggtac tccgcggtgg
       61 ccctggacct ggagggcagg atcgtccagt cccgctccat cccgttctcc tgggacggga
      121 gacacctttc caccgtgttc ctggagatcg tttcggaggc aacccaggcg ctggacatct
      181 cgggggacgg tctcctgggt atcggcgtgg ggctctccgg catcgtggat tcgtcgacgg
      241 gcaccatccg ccagtccctg ccgctggggg tcacgtcacc cctggacttc tactcctcca
      301 gcgcgcggct catcggcaag cctctgtgca tcgacaacga tgccaactgc tgctgctggg
      361 cgcagctcgt ccgtggaagg gatgcgcgac cgcgggagtt cctgttcatc ctgggcgagt
      421 tccgaaagga gcgcgcgggg gacctgggcg gcggggggct gtccgtgggg ctgggcctcg
      481 tgctgggcgg gagggtccac ctgggcgcaa gcagctcggc gggagagttc cgaagcgtct
      541 ttcggacttc ggaaacggga agccagttct ccgtggagcc cgccgtgatg gagcgcgcgg
      601 agaacgatcc ctccgcgttc gagcgggtag cgcgggagct gggccgcaac gtcgcgctcc
      661 tggtcaacac gctggacctc tcccacgtct tcatcggcgg caacatcgag cgcaggcagc
      721 gtgagctggt cccgattctg gaaaaggaga tcgagtccaa ctggccttac ggccgcaaga
      781 gcctcagcat cgaggcgtcc gagctcggag agctcgtggt ggcctatgga gccgcggcca
      841 tgttcctgga gcggctgttc tccctgccgc agatccagga gcactcggta ccggtgggag
      901 tcagcgcggg gtagcggtcc tcatcccgtc cgggcgtact ggcggtcggc ccactccacc
      961 aggccggcga agtaccgctg cgcgtcctgg atcgattccc ggtccggtcc ctggggcgcg
     1021 gaagcattct gtcggatgtc ttcggagagg catcgggccg cccgctggga catgtttctg
     1081 agaacgatct cctgaaccgt ctcgtcctga tccgcgaggg ccacggcgag cgtcggggcg
     1141 tcgatctccc gcagggccat ctggatcacc acgtcgtcga acttcccgta ggtcatgaac
     1201 gctctgaaat cgcgcatcgc gtttcctacc tctggggaca atgtagtgca ccgcgatgcg
     1261 gaaagcttgg cgcggctcag gacggctcct caggtccctc ggccggcgag gctgttctcc
     1321 cttcgacctt tcgtcgcagc cgctggagcc gatgggcgat cgccgtccga tcctgcttcc
     1381 ccagcggcaa tccccagccc aggaggcgct cgatgacctg cagggcaagg tccggcctgc
     1441 gggcccgatg ctccaggtgc ttggcgagct ccacggatgc aagcaggctg tgggagcgct
     1501 ccacggcgga ctgccatacg cgcagcgcct cctcccatcg tccctcccgc ttgaagtgca
     1561 gacccaggct caagcccgct cgggagtcgc catcctcgta cgccttctga agtatggcga
     1621 ttccggactc ggggtccggc cccaggagcc atctcccgag cgcaccctgg tccacgggcg
     1681 cggcggcaag ctcgccgccg agcagcctgc ccagggtgcc gtagatgcgg gccagggacg
     1741 tgatgtccgt gacgttgtgg tcgaagacca gggacaaccg gtccggcctg ccggtccgca
     1801 ggaagtcgta ccagatgccc gggatctcca tgccgggcac gtcatcccca cggtcaaccc
     1861 ccaggatggc cgactcgatt ccgcggaggc tgcagtcgga gctgaccgac ttccagagac
     1921 gacgcgcgtg gtacagcagg tccacgtgga gccaggactc ctcatcgatc cgattcatca
     1981 ggaaccggga gcgaaggagg tgagcgtcga agctcctgcc gttgtaggag accagcgccc
     2041 ggtcggaggg gagaagctgc ttgattccca gaaggaactc gggctcaccg gggaaatcgg
     2101 agaggaagag ctgctccacg acgaagtccg ggccttcgga ccagccaacc ccgaacagga
     2161 agatcatggt tccggccccg cccgagagcc ccgtcgtctc cgtgtcgaag aagaggaggc
     2221 caccgggaga cacggcttcc ggggagatgc gcgagggcca cgagggcacc accaggccag
     2281 ggaacgtctg acggcgacgg tagacgaact cgctctcatg gacccaaccg gggaaccggc
     2341 ggtccgggga taccgagggt ggctcggggc gccgcagcga ggcggacggc cgctcccctg
     2401 ccccctcccg gcctcgctgt ctcaggtagt ccaggccact gcgcgcgcgg gcggatgact
     2461 cgtccatcac tcccccagtg ccagcgccca gtcgcgcaga aagccccgca ccgcggcctt
     2521 gggatccgag ctctcggcgg gctcgtcctc cggcgcgccg atgcacgacg ggcacccgcg
     2581 cgcgcagggg cagcggtcta cgacctcacg cgccccgcgc acgatctgcg ggagctgccg
     2641 gatgaaccct tccgagagcc cgatgccgcc cggatactgg tcgaagacgt agaggcacgg
     2701 ccgctcgaag tggggatccc gcacgcgctc ggaaacgccc agatccctcg gatcacacag
     2761 gaggaacacg ggggatacgg tgcgcaggag gacgcccagc cgctggatca ccacgccctg
     2821 gtccgtctcg ttcatcgccg cgaaggcgcg cccgggccga ctgacctcgt cgaacagcag
     2881 catggccgcc cgcgtgtgca tctcgtccgc cgggaggttg atatcgccgt agccgatgtt
     2941 ctcgttggtg tggtacttca gcttcttgaa cttcgtggcc tgggtccgaa ccaggatgtc
     3001 tcccgagacc acggggatac cggcgacctc tctctggtcg tcctgcgaga ggaccttgat
     3061 gtccgacttc atgatggagt cggtccagta gtcggtatcc gtggtctcca cgaagcagcg
     3121 ccggttggcg atgtccagct tcgtcaccat gaactgctcc cccaggtgga ggtagatggc
     3181 gtggtcgaag atgaggagct tggccgatga caggtccatc tctccgatca cctcgtccct
     3241 gccccgcgtg gtgtccacga tgatcacgtt ctccggcgtg ctggtgcgca gggatacgtt
     3301 ctccgcgggg taggcctggt ccgaccagta ccacttggat gccgcgtgac ggaccacgcc
     3361 ctgctccgaa aggtatccca ggagatccgc ggtgccttcg ccgaatccct ccgagtcctc
     3421 gaagggaagc tcgaacacgg cgcacttgag ctggtcggtc tggatgaaca cgttgcccgg
     3481 atcgatgtag ccgcactctg gggaccggga gaagaagtac tcggggtgct ggatcacgaa
     3541 ctggtccgtg gcggaggatg acgccaccag gatggagagg gagaccgtgg cacgccgacc
     3601 ggatcgtcca gcctgctgcc aggtggagga gatggtgccg ggaaagcccg cgatgatcga
     3661 cgcatccagc ccgccgatat cgatgcccag ctccagcgcg ttggtggaga ccacgccctg
     3721 gatgctccca tcgcgaagcc ccttctcgat ggcccgacgc tcgttcggga gatagccgcc
     3781 ccgatagctt tccacccgga tgccgccgtc gtccgtgtag aagttcctga gggagtcccg
     3841 gatataggtg gcgatgagct cggtgcggat ccgggaccgg gcgaacacga tggtcttcac
     3901 gcccgccttg agcagccgcg tggccagccg ctggctctcc agcaccacgc tgcggcggat
     3961 cccctggacc cggtccacca acggcgggtt gtacagcacg aagtggcgct cgccggaggg
     4021 cgcgccgttc ctgtcgatga gctgcacgtc ctcctccagg atccggcggg ccagctcatg
     4081 ggggttgccg atggttgccg agcagcaaat gaactggggc tttgccccgt agaacgcggc
     4141 gatccgcttc agacggcgga tcacgctcgt catgtgggac ccgaacacgc cccgatagga
     4201 gtgcatctcg tcgatcacca cgaaccgcag gtgccgcagc gccttgatcc acttggtgtg
     4261 gttggggagg atgcccgtgt ggagcatgtc cgggttggtg atgacgatcc ggccttcgtc
     4321 ccgggccgag atccggatgg aggtgggcgt gtctccatca taggtgaaga ccttgacggg
     4381 gatctcccca cccagcacca cctcgttcag ctcggattgc tggtcctggg agagcgcctt
     4441 ggtggggaaa aggtacaggg cccgggtatc cgggtcttcc aggatggact ggagcaccgg
     4501 gaggttgtac gccagggtct tgccggaggc tgtcgggctc acgatgacca cgctcttgcc
     4561 ctggcggacc gcctcccagg tctccgcctg gtggctgtag atccgggtga tgccgcggga
     4621 agcaagggcg gcgcggatcc gcgagtccac gccggcgggg atgtccacgt acctgcccgg
     4681 ttgggccggg accacctccc agtgggtcac acgatccatg aactctcggt cccgcttcag
     4741 ggatccgatc atatcggcga gcatgctccc ctccctgggt tgcttcccct ctatagtatc
     4801 atgcgcggcc ggacttgaca cctgccgttg cgcggaacga tgcttttccc cgggggaagc
     4861 gaagatggac aaaatcgttt cgatcgtact tgccggcggc aaggggcaac gccttcaccc
     4921 gcttacggca aagcgctcca agccggcggt cccctttggc ggcaagttcc gcctggtgga
     4981 cattcccatc tccaactgca tgcacgcggg gtttcgagag atctacatcc tgacccagta
     5041 caactccgcg tccctccacc ggcacatcaa caacacctac cggttcgacg cgttcggccg
     5101 cgggttcgtg gaggtgctgg ccgccgttca gacccccgag tccgaggagt ggtaccaggg
     5161 cacggcggac gcggtgaggc agaacttcct gcacttcgcc gaccactccc cgacgcacta
     5221 cctcatcctg tcgggggacc agctctaccg catggacctc catgcgttct tcgacgccca
     5281 cgtgcagtcc aagtccgacg tgacggtggc ctgcctgccg gtcaccaagg agaaggcgag
     5341 cagcctcggc atcctgcagc tcgatgagcg ctaccatatc acgcgcttcg tcgagaagcc
     5401 gcccgcggac tccgacatgt cctccctggc ggtgcctcgc cggatccgcg aacgggaaga
     5461 gctggacatc gagggagatg cgtacctggc ctccatgggc atctacgtct tctccgcccc
     5521 cgcgctcgcc aaggccctgg acaacgacct cacggacttc ggctcccagg tgatcccggc
     5581 ctccatcgag aagatgaagg tctccggg
//
LOCUS       DASRZS010000247         8683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1676076, whole genome shotgun sequence.
ACCESSION   DASRZS010000247 DASRZS010000000
VERSION     DASRZS010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8683
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676076"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VFH83_06180"
     CDS             complement(<1..358)
                     /locus_tag="VFH83_06180"
                     /inference="COORDINATES: protein motif:HMM:NF018257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HET6485986.1"
                     /translation="MAVAGAVLMGAPIWGQSMMSASNAKLVPYAGAEKILVIDAATKS
                     ATINLVGSEGSAAYGMNFNNFAKGGMVVRVPLGWSVTVSLVVNSNLKHSAEIVPWAQR
                     EGEDFTPSFAGSDPKDA"
     gene            complement(562..1638)
                     /locus_tag="VFH83_06185"
     CDS             complement(562..1638)
                     /locus_tag="VFH83_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6485987.1"
                     /translation="MQLQYKPDFEQVKTLWNAYWEGEIVRRPLVVAAVPRPGRPRGNL
                     GDRYFHTVHRNDEHLMKQIDLILESTEFLAEAIPHFGPDLGPDQFAAFLGSDFAFSPD
                     SPETNWVRQIVDDWDAVLPLRLQPDNPHWNKLLEVSRLLARHSQGRYLISVADLHGNM
                     DALLALRGSERLCMDFYDHPLEVERAIQDVRKLYQPIYNALFEAGAMGGDRGSVGWAP
                     FWCAGRFATIQCDFLALLSPELSRKYVIPALREEAAFLDRCVYHLDGPGCLPHLDDLL
                     AIEDIDVIQWVPGAGKPLMHEWMDVLKKCQAAGKGLQLYDIHDLATVKRIARELKPEG
                     LLYIVDGCDRDEALRIIDWLEKNT"
     gene            complement(1671..2903)
                     /locus_tag="VFH83_06190"
     CDS             complement(1671..2903)
                     /locus_tag="VFH83_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485988.1"
                     /translation="MAALKELARVSWQPPAATPAACGPYGDHPRLGDEEKREVWRAYR
                     AGTPTRVPVTLGINNRVMLQLPELNPDRLTYREVFTDPAAMLLAQLRTQQYIRSCLGW
                     FHDTETSTPDAWRVSAHYQNISEAAFFGCPLQFREGEVPDTLPAYAGDARNAVFDVDI
                     DHPTERGFFAEGIRMTHRMEEVARKSCFLGIPIVVEPYLPLGTDGPLTVAMNLCGPEI
                     LTDLLDHPSYASQLFDLIVTAAYKRNREMRGYWGVEEKPGEEVGLADDSISLLGHQMY
                     RGALLPWHRAWYDRVDPQRNRKRTIHLCGDAQRHFATIHRELGVTSFDTGFPVDFARL
                     REELGPGVEILGGVEVATLLGGAPQAVFERARAILESGILRGGRFILREGNNLPPGVP
                     WSNLAAMYAAAFRHGTYG"
     gene            2993..3844
                     /locus_tag="VFH83_06195"
     CDS             2993..3844
                     /locus_tag="VFH83_06195"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET6485989.1"
                     /translation="MKEAFLERMKDLPVMPEVAARVLSLKEAGVGFRELESIIKVDPA
                     LTARILKVANSALYARQKEITSLQMAISLLGFTNIRNLVLLVTASSFFPRMKRSAFHQ
                     AFWRHSILSAFLSRNFTQACAGGSAAEEAFIAGLLHDIGQAALFSHAPEQYQRALETE
                     KLGAMSLETIEEQLFEVNHRDLGGALLRKWNFPDLYADAAEQHESLNVTSAHKTVILL
                     VTVACLLAARIGGQVLTPARADLLAQIMPYTCFAAISPESLAQEQAWQLSQDPLYREC
                     QELFGIR"
     gene            complement(3851..7669)
                     /locus_tag="VFH83_06200"
     CDS             complement(3851..7669)
                     /locus_tag="VFH83_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6485990.1"
                     /translation="MRRRSANPLRWAATLAVTGTLCLAALGPSAPAAWSADTPAAKGL
                     EPAFPVENQNLSFTEAEDAVSTNFAREPVLNFGVSGFRALQLNQGSGLQGVGSFYADY
                     VVSLPSTGTWELWYGGSPPGPKDDLYASYASPLTVVVDSGTPLPVTRETVAVVGNYSP
                     SFYWNRVGDLSLSEGRHTIRFAVTEKRRADGRYVFYLDCFFLVRKDGDKRLLDGPLPS
                     VFPSNLDDRSIDTPFLSLDDALIRVRDNPSSVAPLVYVSGLYTMLGDYLNALKYLNRA
                     ALLEPSNSNVLVLIAKNRIWKGDIPEGLTAYRTVLNRDPKQRELWLEAGKVAAWNGQY
                     DQSIGFYRDALAAFPGDLDATVNLGLTYLWAGRGDDAEASFREAQRIAGSDPDRLKEL
                     GRIYRISGYPDRALQAYSAAQSAAPQDLESYLLAIDTLLAAGRKAEADLLRKRIMDTF
                     APSDRLSTYLDSFQQRAGLKDQILAEDEDRLKNNPDDLVLRQILAQSYFWNGLKDKAI
                     AEYRHILANYAYHALTDSQNQNSELLLVIDRSYLLADYFNRVPAMIRAQRDSLGAQSG
                     KLAQAQAARDRARTELASAQTAQSKAKAGKDADAAQTAVTAAQGKLQAAEDAVQKETD
                     ALGQAAASAAGLVAHLQKLSLGVIADTGTTGALSQQDKEAEAAFARSIQANHWRYDRG
                     SWQAELAQDTGSNDLARLVSARVSLADRMVSQARATMAVDIGTRQATTAAYTLVQSSL
                     WAGKIADASHQIEQLSGDPGSSLLPRYFPEIVSLDQTLSAPVPPARADADPPNPATVA
                     SQLGSLLQTVSNQRGQLQRNLSLLHTLYRHSVIRADYAFEQAVSSIRNELGDYYLAGD
                     PPALDAAIGQFRRVLSVDPGNLDATFRLAKVYEWKQDWKSALDAYKAVYTADPGYENV
                     SGLYNRLSRLHASTLSSQAYALADTQHVQWHSEADWSTPFDSALGFDLTYQTDAMRIQ
                     RANVTGGTDASSYQVQDLSMGLPIDLYLANVKLTPWVGGQLVGDSLYQKTGSVVPTDN
                     QFETITAALPYARLDASVGAWNALFLSGTLRWGVEPETLDPSRGAQAWDGSAEANLST
                     QLSFLNAWPFRDTSLRTYARVDYIPAGPQVHENLMYTALQEVTINLLKGGSPYGVVSL
                     TGNVTFQHADYLEPYLYWAPPDVLLAGASLTGSEWIGAPNGDVLGLSLRVYGGSYQQS
                     IFTAPTHELKAELEADITLTTGSSTWSLAILGNGTYNFALAGTPNPWDYWSGYARLGY
                     SVSLPDLLAP"
     gene            7705..>8683
                     /locus_tag="VFH83_06205"
     CDS             7705..>8683
                     /locus_tag="VFH83_06205"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET6485991.1"
                     /translation="MKVSGTPVRKAAEILAFTAVVCVISSFFPTNPGFLEGFFNPFVT
                     LSLFIAVSYGTYYGFLCLAYGALGVALIIPLARSLLSGAGFALPSGWWADLGRRAALP
                     LPVSILEVYILGIMRDALTRRDRTARERLITLSRDKGLLKRQVRALTTANRELEERIS
                     GQEDSITSLFSRIQVLGSLNLGKTVGAILEITTRFLGATRCSIWAHQPEEKSLRFVAG
                     IGERPEDSPAVLADEGTIEGWVVRNSSMFSVKMLLTNDALSRMDAGRNILTMPILAGR
                     RTWGVLNIEEMPFAKYNLYSERLLQVIMALAGPALERAIEFESVVRQEDV"
BASE COUNT         1406 a         2867 c         2916 g         1494 t
ORIGIN      
        1 cggcgtcctt cggatccgag ccggcgaacg acggcgtgaa gtcctcgccc tcccgctggg
       61 cccacggcac gatctcggcg ctgtgcttca ggttggagtt caccacgagg ctcacggtta
      121 cgctccagcc gaggggaacc cgcaccacca tgccgccctt ggcgaaattg ttgaagttca
      181 ttccgtacgc cgcgctgccc tcggagccca ccaggttgat ggtggcgctc ttggtggccg
      241 cgtcgatcac caagatcttc tcggcgcccg cgtagggcac cagcttcgcg ttggaggcag
      301 acatcatgct ctggccccag atcggggctc ccatcaggac ggcacccgcg accgccatcg
      361 ccaccgcgaa ggatggcaga ggccgccggg cgccggatcg tcgtgctgca aacaggcgca
      421 tccgcacccc tttcttttca gatcgtcggc gcggcgcgtc cagcggccgg gccgacgttc
      481 caaaggtaat cacgggaggg cgcggaggga acgatcaggc gggaccgccg ggcacggccg
      541 cacggcgcgg ggtccgactg cctaggtgtt cttctccagc cagtcgatga tccgaagcgc
      601 ctcgtcgcgg tcgcagccgt ccacgatgta caggaggccc tccggcttca gctctcgggc
      661 gatccttttc acggtggcca ggtcgtggat gtcgtagagc tggagacctt tgccggcggc
      721 ctggcacttc ttcaggacat ccatccactc gtgcatcagc ggcttgcccg cgccgggcac
      781 ccactgaatc acgtcgatgt cctcgatggc caggaggtcg tccagatgcg gcaggcagcc
      841 gggtccgtcc aggtggtaca cgcagcgatc caggaaggcg gcctcttccc tcagcgcggg
      901 gatcacgtac ttgcgggaga gctcggggct cagcaaggca aggaagtcgc actggatggt
      961 cgcgaacctg cccgcgcacc agaacggcgc ccagccaacg gacccgcggt ctccgcccat
     1021 ggcgcccgcc tcgaagagcg cgttgtagat cggctgataa agcttgcgca cgtcctggat
     1081 ggcccgctcc acctccagag ggtgatcgta gaagtccatg cacaaccgtt ccgagccccg
     1141 cagggccagg agcgcgtcca tgttgccgtg caggtccgcc acgctgatca aatagcggcc
     1201 ctgcgagtgg cgggccagca ggcgggagac ttccagcagc ttgttccagt gcgggttgtc
     1261 gggttgcagc cgcagcggga gcacggcatc ccagtcgtcg acgatctgcc tcacccagtt
     1321 cgtctccggg ctgtccggcg agaacgcgaa gtccgatccc aggaaggcgg cgaactgatc
     1381 cggccccaga tccggcccga agtgcgggat ggcctccgcc aggaactccg tggactccag
     1441 gatgaggtcg atctgtttca tgaggtgctc gtcgttgcga tgcaccgtgt ggaagtatcg
     1501 atcccccagg ttcccgcggg gacggccggg cctcgggacc gccgcgacca ccagcggacg
     1561 cctgacgatc tccccttccc agtaggcgtt ccacagagtt ttcacctgct cgaaatcggg
     1621 cttgtactgt agctgcatgg ccgggatctt cgcgggaacg cgaccggctg tcaaccgtag
     1681 gtgccgtgac ggaacgcggc cgcgtacatg gccgccaggt tgctccaggg cacaccgggc
     1741 ggaaggttgt tcccctcccg caggatgaag cggccgcccc ggaggattcc ggattccagg
     1801 atggctctgg cccgctcgaa caccgcctgc ggcgcgcccc cgaggagcgt ggcaacctcg
     1861 acgccgccca ggatctccac cccgggcccc agctcctcgc gcagccgggc gaagtccacc
     1921 gggaaccccg tgtcgaagct ggtgacgccc aactcccgat ggatcgtcgc gaagtgcctc
     1981 tgagcatccc cgcatagatg gatggtccgc ttgcgattgc gctgaggatc cacgcggtca
     2041 taccaggccc ggtgccaggg cagcagggct ccccggtaca tctggtgacc caggagggag
     2101 atggaatcat cggccagtcc cacctcttcc ccgggctttt cctcgacgcc ccagtaccct
     2161 cgcatctcgc gattgcgctt gtaggcggcg gtgacgatga gatcgaaaag ctgcgaagca
     2221 taggacgggt ggtccagcag atccgtcagg atctccgggc cgcagaggtt catcgccacc
     2281 gtcagcggtc cgtcggtccc cagcggcagg tagggctcca ctacgatggg gatccccagg
     2341 aagcagctct tgcgcgccac ctcctccatt cggtgggtca tgcggatgcc ctccgcgaag
     2401 aatcccctct ccgtcgggtg gtcgatgtcc acgtcgaaga ccgcgttgcg ggcgtctccc
     2461 gcgtaggcgg gcagcgtgtc cggcacctcg ccctcgcgga actgcagtgg gcagccgaag
     2521 aaggcggcct ccgatatgtt ctggtagtgg gcgctgacgc gccacgcgtc cggagtggag
     2581 gtctccgtgt cgtggaacca ccccaggcac gagcggatgt actgctgcgt gcgtagctgg
     2641 gccaggagca tggccgcggg atccgtgaac acttcgcggt aggtgagccg gtccgggttg
     2701 agctcgggga gctggagcat gacccggttg ttgatgccga gggtcaccgg cacacgggtc
     2761 ggcgtgccgg cccggtaggc gcgccagacc tctcgcttct cctcgtcccc aaggcgcggg
     2821 tggtcaccgt agggtccgca agcggccggc gttgccgccg ggggctgcca cgagacgcgc
     2881 gccagttcct tcaacgcagc cataccgtgc tccgctggaa agtgtagcac gcggcgacgg
     2941 cggggaccta atgcggggag cgtgtcattc cgatacagta gggggagaga acgtgaaaga
     3001 ggcctttctc gagcgcatga aggacctgcc ggtcatgccg gaggtggcag ccagggtcct
     3061 gagcctcaag gaagccgggg tcggcttccg ggagctggag tccatcatca aggtggaccc
     3121 ggccctgacc gctcggatcc tcaaggtggc aaactccgcg ctctatgccc gacagaagga
     3181 gatcaccagc ctccagatgg ccatctccct gctggggttc accaacatcc ggaacctggt
     3241 gctcctggtc accgcttcca gctttttccc ccggatgaag cgctccgcgt tccaccaagc
     3301 cttctggcgc cactcgatcc tcagtgcctt cctgtcgcgc aacttcaccc aggcctgcgc
     3361 tgggggttcc gcggcggaag aggcgttcat cgccggactg ctgcacgaca tcgggcaggc
     3421 cgcgctcttc agccacgcgc cggagcagta ccagcgggcg ctcgagacgg agaagctcgg
     3481 agccatgtcc ctggagacca tcgaggagca gctctttgag gtgaaccacc gggatctcgg
     3541 cggcgcgctg cttcgcaagt ggaacttccc cgacctctac gccgacgcgg ctgaacagca
     3601 cgagtccctc aacgtcacct cggcgcacaa gaccgtcatc ctgctggtca cggttgcctg
     3661 cctgctggcg gctcgcatcg gaggccaggt cctgaccccg gctcgcgcgg acctccttgc
     3721 ccagatcatg ccatacacgt gcttcgccgc catctcgccg gaatccctgg cacaggagca
     3781 ggcgtggcag ctctcgcagg accccctcta ccgggagtgc caggagctct tcgggatccg
     3841 gtagtcccct ctacggggcc agcaggtccg gcaggctcac cgagtacccc agccgggcat
     3901 accccgacca gtagtcccac ggattgggcg tccccgcaag cgcgaagtta taggtgccgt
     3961 tccccaggat cgccaggctc cacgtggagc tccccgtagt cagggtgata tccgcttcga
     4021 gctcggcctt cagctcgtgg gtgggcgccg tgaagatcga ctgctggtag cttccgccgt
     4081 agacgcggag gctcagcccc agcacatccc cgttgggcgc gccgatccac tccgaccccg
     4141 tcaggctggc gccggccagc agcacatcgg gcggagccca gtacaggtac ggctccaggt
     4201 aatccgcatg ctggaaggtg acgttcccgg tcagcgacac gacgccgtac ggggagccgc
     4261 ctttcaggag gttgatcgtg acctcctgaa gcgccgtgta catcaggttc tcgtggacct
     4321 ggggacccgc cggaatgtag tccacccggg cgtacgtgcg aagcgacgtg tcacggaacg
     4381 gccaggcgtt gaggaaggag agctgcgtgc tgaggttcgc ctccgcggat ccgtcccacg
     4441 cctgcgcgcc tcgagagggg tccagcgtct cgggctccac gccccagcga agggtcccgc
     4501 tcaggaacag cgcgttccag gcgcccacgg atgcatccag cctggcatac ggcagcgccg
     4561 ccgtgatggt ctcgaactgg ttgtcggtcg gcacgacgga cccggtcttc tggtacaggc
     4621 tgtctcccac gagctggccg ccgacccagg gggtcagctt cacgttggcc aggtagagat
     4681 cgatcggaag acccatggac aggtcctgga cctggtagga ggaggcgtcc gtgccgccgg
     4741 tcacgttcgc cctctggatg cgcatggcgt ccgtctggta ggtgaggtcg aacccgagcg
     4801 cgctgtcgaa cggcgtgctc cagtccgctt cggagtgcca ctgcacgtgc tgggtgtccg
     4861 ccagggcata cgcctgggag gagagggtgg acgcgtggag acggctcagc cggttgtaca
     4921 gcccggacac gttctcgtat ccggggtccg ccgtgtacac ggccttgtag gcgtccagcg
     4981 ccgacttcca gtcctgcttc cactcgtaga ccttggccag tcggaaggtg gcatccaggt
     5041 tgccggggtc caccgacagg accctccgga actgcccgat ggcggcatcc agggccgggg
     5101 gatcaccggc aaggtagtag tctcccagct cgtttcgaat ggaggagacc gcctgctcga
     5161 acgcgtaatc ggcgcggatc accgagtggc gatacagggt gtgcagcagg gaaaggttcc
     5221 gctggagctg tccgcgctgg ttcgatacgg tctgaaggag cgatcctagc tgggacgcca
     5281 ccgtggcggg attcggcgga tccgcgtccg cgcgggcggg cgggaccggc gccgaaaggg
     5341 tctgatccag agacacgatc tccgggaagt accgcggcag cagcgagctc ccggggtcac
     5401 cggagagctg ctcgatctga tgggaggcgt cggcgatctt gcccgcccag agcgagctct
     5461 gcaccagggt gtacgcggcg gtcgttgcct ggcgcgtgcc tatgtccaca gccatcgtgg
     5521 cgcgggcctg ggaaaccatc cggtcggcaa gggagacgcg cgcggacacg agccgcgcca
     5581 ggtcgttgga cccggtgtcc tgggcgagct ccgcctgcca cgaaccccga tcgtacctcc
     5641 aatggttcgc ctggatggag cgcgcgaacg cggcttccgc ctccttgtcc tgctgggaaa
     5701 gggcgcccgt ggtgccggtg tccgcgatga cgcccaggga gagcttctgg aggtgggcca
     5761 cgaggccggc ggccgaagct gccgcctggc ccagagcgtc cgtctccttc tgcacggcgt
     5821 cctccgcggc ctgaagcttc ccctgggcag cggtgaccgc cgtctgcgcc gcgtcggcgt
     5881 cctttccggc cttggccttg gactgcgccg tctgggcaga tgccagctcg gtcctggcgc
     5941 ggtccctggc cgcttgcgcc tgagccagct tgccggattg cgcgcccagg gaatcccgct
     6001 gggcacggat catggcgggg acccggttga aatagtccgc caggagatag ctgcggtcga
     6061 tgaccaggag gagctccgag ttctggttct gggagtccgt cagggcgtga taggcgtagt
     6121 tggccaggat gtggcgatac tcggcgatgg ccttgtcctt cagaccgttc cagaagtagc
     6181 tctgggccag gatctggcgg agcaccaggt cgtccggatt gttcttgagc cggtcctcgt
     6241 cctcggcaag gatctggtcc ttcagacccg ccctttgctg gaaggagtca aggtacgtcg
     6301 agagacggtc ggaaggcgcg aaggtgtcca tgatgcgctt gcgcagcagg tccgcctcgg
     6361 cctttctccc ggcagccaac agcgtgtcga tggccaggag gtaggactcc aggtcctgag
     6421 gcgccgcgct ctgcgccgcg gagtaggcct ggagcgcccg gtccggatac ccgctgatgc
     6481 gatagatcct gcccagctcc ttgagccgat ccgggtcgga gcccgcgatc cgctgggcct
     6541 cccggaagct ggcttcggcg tcatcgcccc gtcccgccca caggtacgtc aaccccaggt
     6601 tcaccgtcgc gtcgaggtca ccggggaacg ccgcaagggc gtcccggtag aatccgatgg
     6661 actggtcgta ctgcccgttc caggccgcca ccttgccggc ctccagccaa agctcgcgct
     6721 gcttgggatc ccggttgaga acggtccgat acgccgtgag cccttctgga atgtccccct
     6781 tccagatccg gttcttggcg atcagcacca ggacgttcga gttggacggc tcgagcaacg
     6841 cggcgcggtt caggtacttg agcgcgttca ggtagtctcc cagcatggtg tacaggccag
     6901 agacgtacac cagcggcgcg acgctcgatg ggttgtcccg gacgcggatg agcgcatcgt
     6961 ccagggacag gaacggcgtg tcgatggacc ggtcgtccag gttcgacggg aacacgctgg
     7021 gcagcggtcc gtccaggagc ctcttgtccc cgtccttgcg caccaggaag aagcagtcca
     7081 ggtagaagac gtaccgtccg tcggctcgcc gcttctcggt gaccgcgaag cggatggtgt
     7141 gccgtccttc cgagaggctc aggtctccca cccggttcca gtagaaggaa ggggagtagt
     7201 tgcccaccac ggccacggtc tcccgggtca ccggcagggg cgttccactg tccaccacca
     7261 cggtgagcgg tgacgcatag gacgcgtaca ggtcgtcctt gggtcccggc ggcgagccgc
     7321 cgtaccagag ctcccaggtg cccgtggacg ggaggctgac cacgtagtcg gcatagaacg
     7381 atccgacgcc ctgcagaccg ctgccctggt tgagctgaag ggcgcggaat ccggagaccc
     7441 cgaagttgag cacgggctcc cgggcgaagt tggtggagac ggcgtcctcc gcctcggtga
     7501 aggagaggtt ctggttctcc accgggaatg ccggctccag tccctttgcg gcgggcgtgt
     7561 ccgcgctcca ggccgccgga gcggacgggc ccagcgccgc aagacagaga gtccccgtca
     7621 ccgcaagcgt ggccgcccat cgaagcggat tcgcggatcg ccttctcatg acatcgtctc
     7681 cccgttcatg ctactgtctc tttcgtgaaa gtcagcggta caccggtccg aaaggctgcg
     7741 gagatcctgg ctttcaccgc cgtagtctgc gtgatcagct cgttcttccc taccaatcca
     7801 ggcttcctgg aggggttctt caatccgttc gtcaccctct ccctctttat cgcggtttcc
     7861 tacggtacgt attacggttt cctctgcctg gcgtacggcg ccctgggggt ggcgctgatc
     7921 atccccttgg cgcgcagcct cctctccgga gcgggatttg ccctgccctc gggatggtgg
     7981 gccgacctgg gtcggcgggc ggcgctgccc ttgccggtct cgatcctgga ggtctacatc
     8041 cttgggatca tgcgagacgc cctcacgcgg agggatcgca cggcacggga acgcctcata
     8101 accctatccc gcgacaaggg gcttctcaag aggcaggtcc gcgcgctcac cacggccaac
     8161 cgtgagctgg aagagcgcat ctccggccag gaggacagca tcacctcgct gttctcgcgg
     8221 atccaggtgc tgggctccct caacctgggc aagacggtag gggccatcct ggagatcacg
     8281 acgcgtttcc tgggggccac gcgttgctcc atctgggcgc atcagccgga ggagaagagc
     8341 cttcggttcg tggcgggaat cggggagcgg ccggaggaca gccccgcggt gcttgcggac
     8401 gagggcacca tcgagggatg ggtggtgcgc aacagcagca tgttcagcgt caagatgctg
     8461 ctcaccaacg atgccctgtc ccggatggac gccggacgga acatcctgac catgcccatc
     8521 cttgccgggc gccggacgtg gggcgtgctc aacatcgagg agatgccgtt cgccaagtac
     8581 aacctgtact cggagcggct gctccaggtg atcatggccc tggcgggccc ggccctggag
     8641 cgtgccatcg agttcgagag cgttgtgcgt caggaggacg taa
//
LOCUS       DASRZS010000248         6834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5141771, whole genome shotgun sequence.
ACCESSION   DASRZS010000248 DASRZS010000000
VERSION     DASRZS010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5141771"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFH83_06210"
     CDS             <1..578
                     /locus_tag="VFH83_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6485992.1"
                     /translation="ITWSKTGWSDLLKSVHEGRYDIAANGITITADRKEQVDFSDPYL
                     VSEQRMVARSDEGRFSSAAQLGSGTTLLIGAQTGAAGFYAAAYNVLDGDEKSARIKLF
                     PSAALAVKALLAGTVDCVLLEEAAARGFVNAGGGKLKVVGEALAREPLGLAVTPGSEL
                     TASIDAAIRSMKDDGTLERLATRWFYEKGTP"
     gene            575..1717
                     /locus_tag="VFH83_06215"
     CDS             575..1717
                     /locus_tag="VFH83_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6485993.1"
                     /translation="MTPRLRLLRALRREPVDRIPVSTYEFHPFGGCWAAEEPSYRPLL
                     ELQRERGDTFVFAPSATALLGDPNQIRTGVERTQREGITRQTVPTPRGDLTSVSRTDH
                     GVATSWTLKHFIETDEDIERFLSIPYRFVAPDLPEVRRLEAVAGEQGLLLFSIGDPLG
                     VITGLCDYTFFVTRIATDAGLARELLDRGSRFIRDTVQWINAGFRDVGVRFWGPEYCG
                     APLMDPRKHFRTLVVERLAPLVGLVHAGGNVALVHCHGRLDALLEMILELGADALEPL
                     EVLPATTADLTMADIRRRVGDRMCLAGGMQAVDLDTGTPELIRRRVRAIVEQAGTQGL
                     ILLPTSTPLGIPLPSRVVENYRAMFEEAGAVGPSGAARPTGAAAGP"
     gene            1912..3579
                     /gene="ilvB"
                     /locus_tag="VFH83_06220"
     CDS             1912..3579
                     /gene="ilvB"
                     /locus_tag="VFH83_06220"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015614608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HET6485994.1"
                     /translation="MKLSGASLLIELLKRQGVEIVAGIPGGANLPLYDAQGQSSIRHI
                     LVRHEQAGGFVAQGMARSTGTAAACFATSGPGATNLLTAIADAKLDSIPIVAVTGQVP
                     SKLIGTDAFQEVDTYGMSIPVTKHNFLVRSAAELLEVVPEAFRVALSDRPGPVLIDLP
                     KDVQQAAIEVDGWPEPGARETPSGPDGAVMARMARTIAAAERPLLYIGGGAVASAAEE
                     EIRTLSASCSIPVVSTLMGLGVMPLEDPLYLGMIGMHGSRATNFAVDRCDLLIALGVR
                     FDDRATGSIAGFAPKAKVIHLDIDEAEIGKIRRPDISAACDVKQALRRLLHLVGTADR
                     PAWRAEIADLRAKYSLPEPTDESHPGTLMKRLAAAAPENAVVTVDVGQHQMWAAQWWP
                     VRHARSFLTSGGLGTMGFALPAAMGAALAHPDRPAVVLVGDGSMLINIQELATIAENE
                     LDVRICVLNNGHLGLVRQQQELFYGKRYVASRFASRPDFAAIARGFGIRGCTLTLPHD
                     QTALARALSERGPVLLDIALPETENVLPMVPPGKGNTEAIWTQEASS"
     gene            3576..3872
                     /locus_tag="VFH83_06225"
     CDS             3576..3872
                     /locus_tag="VFH83_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET6485995.1"
                     /translation="MTVLPNTIVKLNVKNHPGAMSHITGLFSRRGFNLEGILCGPVNG
                     GGDESVIYLLVRNDDRLEQIVKQLAKLYDVRSVTVQDDFDHTVFNRIEQLLGDV"
     gene            complement(3915..4547)
                     /locus_tag="VFH83_06230"
     CDS             complement(3915..4547)
                     /locus_tag="VFH83_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET6485996.1"
                     /translation="MIAESVLQSRVLVLNSLYQPINVTSLKKALCKLVKSVAEVVHVD
                     DGKYSSYSLVTWAQVSDLRRQMQDLGTEDELIGSGGFSLVVPRIIRVLTYSKSRKHPV
                     RLNRKNIFARDKRTCQYCGKRFPSERLNLDHVVPRSQGGQEAWENLVCACFRCNSRKG
                     GRTPFQAGMKLIRQPVRPPATTLRVTIGDRKYKDWEAFVSDAYWEIDLQD"
     gene            4867..>6834
                     /locus_tag="VFH83_06235"
     CDS             4867..>6834
                     /locus_tag="VFH83_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6485997.1"
                     /translation="MKKTTVFLLCFVLASAFVAVAASPTAPKGVQEWNSPADYQKATG
                     KKIPRYNESPMLKDLVAKGALPSVDKRLPAEPLVVQPLEEIGQYGGQIVGYSTTQGLG
                     HIDWGTTLALFQLDGLLRPGPDSTGLVPNVCSAYEMAKDGKSIVIHLRKGIRWSDGVP
                     FSADDILFTYNDIIMDEKVALQTGMAAQKSYYSPTSDPAKIEKIDDFTVRFTFPAPYP
                     IFPLQMVVECGIEGKILFPKHYLQKFHIKYNPDADTLAKSKGLNHWWELFPTMADVQT
                     GPQNTTVGWPTLRAYVLKEVQPGYLIGERNPYYWKVDTAGNQLPYVDKIMVIQNLDPQ
                     VVTSKIIAGDFDYANGAIEDLQLLKKNEGAGKYTTYLWKNALTSVFGFEFNLTYKDDP
                     VLASIFRDVRFRRAMSLAINRSEINDVAFNGLAVPGQASCMPSMTRYYEDSFGKAYAQ
                     YDVSQANSLLDQMGLAKKDGDGFRLRSDGKRLSVLIEYADGFSAPIFELTKEYWEKVG
                     VEVTLKPESAKLIFERYPTNVVQVGGWQDDRGTTEDSLIQYPSRYVPCGWPPYNNEWP
                     LWLQWAQSNGKQGEEPIAEVKKNLDALRTMGTTTSDAERVAAAKQILRSQAENLWVIG
                     TVGYPPFIRIVRSTIHNVPTDGFMGTWGDEAY"
BASE COUNT         1224 a         2193 c         2264 g         1153 t
ORIGIN      
        1 ggatcacctg gagcaagacc gggtggagcg accttctgaa gagcgtacac gaggggcgct
       61 acgacatagc ggccaacggg atcacgatca cggcagaccg caaggagcag gtggacttct
      121 ccgatccgta cctggtcagc gagcagcgca tggtggctcg ctctgacgaa ggcaggttct
      181 cgagcgcggc gcagctcggc tctggcacga ccctcctcat cggcgcccag acgggagccg
      241 cgggcttcta tgcggcggcc tacaatgtgc tggatggaga cgagaagagc gcccggatca
      301 agcttttccc ttcggctgcg ctcgcggtca aggcgctcct ggccggaacc gttgactgcg
      361 tcctgctgga agaggccgcc gcgcgcgggt ttgtgaacgc cggcggcggg aagctgaagg
      421 tcgtgggaga ggcgctggcg cgggagcctc tggggcttgc cgtcacgccg ggctccgagc
      481 tgaccgcttc gatcgacgcc gcgatccgca gcatgaagga tgatggaacg ctggaacgcc
      541 tggccacccg gtggttctac gagaaaggaa cgccgtgact ccccgcctga ggctcctccg
      601 cgcgctgcgg cgagaacccg tggaccgcat cccggtcagc acgtacgagt ttcacccctt
      661 cggcggctgc tgggccgcgg aggagccttc ctatcggccg ctgctcgagc ttcagcggga
      721 gcggggagac acgttcgtgt tcgccccgtc tgccaccgcg ctcctcgggg atccgaacca
      781 gatccgcacc ggcgtggagc ggacccagcg ggagggaatc acccgccaga cggtacccac
      841 cccgcggggg gacctcacct ccgtgagccg gacggaccac ggggtagcta cgagctggac
      901 cctcaagcac ttcatcgaga ccgatgagga catcgagcga tttctgagca tcccgtaccg
      961 gttcgtggcg ccggacctcc cggaggtaag gcggctggaa gcggtcgccg gggagcaggg
     1021 tcttctcctg ttcagtattg gggatccgct gggggtcatc accgggctgt gtgactacac
     1081 cttcttcgtc acgcggatcg ccacggacgc cggattggct cgagagctcc tggaccgcgg
     1141 ttcgcggttc atccgcgaca cggtccaatg gatcaacgcc ggctttcggg acgtcggcgt
     1201 acggttctgg ggcccggagt actgcggcgc cccgctgatg gatccccgaa agcacttccg
     1261 taccctggtg gtggaacgac tggccccgct ggtgggattg gtccacgccg gcgggaacgt
     1321 tgccctggtc cactgccacg gtcggctgga tgcgctcctc gagatgatcc tggagctcgg
     1381 tgccgatgcg ctggagccgt tggaggtgct tccggccacc acggcggact tgacgatggc
     1441 cgatatccgg cgccgcgtcg gtgaccgcat gtgccttgcc ggcggcatgc aggcagtgga
     1501 cctggacacg ggcaccccgg agctgatccg ccggcgcgtg cgcgcgatcg tggagcaggc
     1561 gggcacccag ggcttgatcc tcctgcctac ctcgacgccg ctcgggatcc cccttccctc
     1621 ccgagtcgta gagaactatc gggcgatgtt cgaggaagcg ggagcggtag ggccttcggg
     1681 agccgcaaga ccgacagggg ccgcagccgg gccttgaccg tcgtgcctcc cctgggtacc
     1741 tttggcgtgc acatggaaac caggcgcctc ggacaggcac ggctcgcgct cctcgtagta
     1801 gtagtactcg cagggggcac gaccgtgtgt gcaccgtagg cttatcgaag gaaacggata
     1861 agcccctctc ggtggacaca ccgagggggg cttttttttg caggaggagt catgaaactc
     1921 agcggcgcgt ctctcctcat cgagctcctg aagcgccagg gggtcgagat cgtggcgggg
     1981 atcccggggg gcgccaacct tccgctctac gacgcccagg gtcagagcag catccgccac
     2041 atcctggttc gacacgaaca ggctgggggg ttcgtggccc agggcatggc ccgatccacg
     2101 ggcaccgcgg ccgcctgttt cgccacctcg ggaccggggg cgaccaacct cctgacggcc
     2161 atcgcggatg ccaagctcga ctcgatcccg atcgtggcgg tgacggggca ggtcccctcc
     2221 aagctgatcg gcaccgacgc gttccaggaa gtggatacct acgggatgag catcccggtc
     2281 accaagcaca acttcctggt ccgaagcgcc gccgagctcc tggaggtcgt tcccgaggcc
     2341 tttcgggtgg ccctctccga cagacccggg ccggtcctga tcgatctgcc caaggacgtg
     2401 cagcaggcgg ccatcgaggt ggatgggtgg ccggagcctg gcgcgcggga gaccccgtcc
     2461 ggaccggatg gcgccgtgat ggcgcggatg gcgcgcacca tcgccgccgc cgagcgcccg
     2521 ctcctgtaca tcggcggagg cgcggtggcc tccgccgccg aggaggagat ccgcacgctt
     2581 tccgcttcct gctccatccc ggtggtgtcc acgctcatgg gcctgggcgt gatgccgctg
     2641 gaagaccccc tctatctcgg gatgatcggg atgcacggga gccgggccac caacttcgcg
     2701 gtggaccgct gcgacctgct catcgcgctc ggggttcggt tcgacgaccg ggcaaccggc
     2761 tccatcgccg gcttcgcgcc caaggccaag gtcatccacc tggatatcga cgaggcggag
     2821 atcggcaaga tccgaagacc cgacatctcc gcggcctgtg acgtgaagca ggcgctccga
     2881 aggctgctgc acctggtggg gaccgcggac cgtcctgcct ggcgcgcgga gatcgcggac
     2941 ctgcgcgcca agtactcgct gccggagccg accgatgagt cccatcccgg cacgctgatg
     3001 aagaggctgg cggccgcggc tccggagaac gccgtggtaa cggtggacgt gggccagcac
     3061 cagatgtggg ccgcccagtg gtggccggtc cgccacgcgc ggagcttcct cacctccggc
     3121 ggtctgggaa ccatggggtt cgccctaccc gcagccatgg gagcggcact ggcccacccg
     3181 gaccgtcccg ccgtggtcct ggtgggggac ggctcgatgc tcatcaacat ccaggagctg
     3241 gccaccatcg cggagaacga gctggacgtg cgcatctgcg tgctcaacaa cgggcacctg
     3301 ggcctcgtgc gccagcagca ggagctgttc tacggcaagc gctacgtggc ctctcggttc
     3361 gcgtcccgac cggacttcgc ggccatcgcg cgcgggttcg gcatccgagg atgcacgctg
     3421 accctgccgc acgatcagac cgccctggcg cgcgcccttt cggagcgggg acccgtcctt
     3481 ctggatatcg cgctgccgga aacagagaac gtcctcccca tggtgccgcc ggggaagggg
     3541 aacaccgagg ccatctggac ccaggaggca agctcatgac cgtcctgccg aacaccatcg
     3601 tgaagctgaa cgtgaagaac caccccgggg cgatgtccca catcacgggg cttttctccc
     3661 ggcgcggctt caacctggag gggatccttt gcggtccggt gaacgggggc ggggacgaga
     3721 gcgtgatcta cctgctggtg cgaaacgacg accggctcga gcagatcgtg aagcagctag
     3781 ccaagctcta cgacgtgcgg tccgtcacgg tgcaggacga tttcgaccac accgtcttca
     3841 accggatcga gcagctcctg ggggacgtgt aacgacgcgg gggccgaagc ggccgcgcgc
     3901 cgcgcgccgg cgcctcagtc ctggaggtcg atctcccagt aggcgtccga cacgaacgcc
     3961 tcccaatcct tgtacttgcg gtccccgatg gtgacccgaa gcgtggttgc tggcggccgc
     4021 accggctgcc ggatgagctt catccccgcc tgaaaaggcg tccgcccgcc ctttcgggag
     4081 ttgcagcgga agcaggcgca caccaggttc tcccacgcct cctgacctcc ctggctgcgg
     4141 gggaccacgt ggtccaggtt gagcctctca ctggggaacc gtttgccgca gtactggcag
     4201 gtccgcttgt cccgggcaaa aatgttcttg cggttcagcc gcaccggatg tttgcggctc
     4261 ttcgagtagg tgagcacgcg gatgatccgg ggaaccacga ggctgaaccc gccgctcccg
     4321 atgagctcgt cctccgtgcc caggtcctgc atctgccgcc gaaggtcgct cacctgggcc
     4381 caggtgacca ggctgtagct gctgtacttc ccgtcatcga cgtgcaccac ttccgccacg
     4441 gacttcacca gcttgcacag cgccttcttc aggctcgtga cgttgatggg ctggtagagg
     4501 ctgttgagga cgaggactcg ggactgtagc acgctctcgg ctatcatgct gtggctcctg
     4561 cgcatacccg gacccggagt tgcgcgcggc ggccaagcat actccctccg aggcgcggca
     4621 tgccagaggt caagcgcggg cgggcgaggg aagcgacctg cgcctcgccg acggaaccgg
     4681 tcgcagacgg aacctgtcgc ggccggggaa tttgacagcg gccgacagct tgtgatacga
     4741 tcgctttgct attcgttgtg cacagtatgt gtgccaactc aaacctgatc cgacacgctc
     4801 gtccctctta tcgtcattag cgaaagtgcg cgtatgcgca tcttattccg ggatagggag
     4861 atgggtatga agaaaaccac ggtgtttttg ctgtgcttcg ttctggcatc ggcctttgtg
     4921 gccgtggccg cgagccccac cgcgccaaaa ggggtgcagg aatggaactc gccggcggac
     4981 taccagaagg caacggggaa gaaaatcccc agatacaacg aatcgccgat gctgaaggac
     5041 ctggtggcca agggcgccct tccctccgtg gacaagaggc tgccggcgga acccctggtt
     5101 gttcaaccgc tggaggagat cgggcagtac ggcggacaga tcgtcggcta cagcacgacc
     5161 caggggttgg gtcacatcga ctggggcacc acgctggcgc tgtttcagct cgacggtctc
     5221 ttgaggccgg gcccggatag cacgggcctc gttccgaacg tctgctccgc gtatgagatg
     5281 gccaaggatg gaaagtccat cgtcattcac ctgcgcaagg gcatccggtg gtcggatggg
     5341 gttcctttct cggcggacga catcctcttc acctacaacg acatcatcat ggatgagaag
     5401 gtggcgctgc agacggggat ggccgcgcag aagagctact acagcccgac cagcgatccg
     5461 gcaaagatcg agaaaatcga cgatttcacc gtcaggttca cgttccccgc gccctatccg
     5521 atcttcccgc tgcaaatggt cgtggagtgc gggatcgaag ggaagatcct gttccccaag
     5581 cactacctgc agaagttcca catcaaatac aacccggatg cggacacgct tgccaagtcg
     5641 aagggtctca accactggtg ggagcttttc ccgaccatgg cggacgtcca gaccggtccc
     5701 cagaacacga cggtcggctg gccgaccctg cgcgcgtatg tcctgaagga agtgcagccc
     5761 ggctatctca tcggagagcg aaacccgtat tactggaagg tggacaccgc aggaaaccag
     5821 cttccctacg tcgacaagat catggtcatc cagaacctgg atccccaggt ggtgacctcg
     5881 aagatcattg ccggtgattt cgactatgcg aacggggcta tcgaggacct ccagcttctg
     5941 aagaagaatg aaggcgccgg gaagtacacc acctacctgt ggaagaacgc ccttacgtcg
     6001 gtcttcggtt tcgagttcaa cctgacctac aaggatgacc cggtcctggc ctccatcttc
     6061 cgtgacgtga gattccgccg cgccatgtcc ctcgcgatca accgcagcga gatcaacgat
     6121 gtcgccttca acgggcttgc ggttcccgga caggcgtcgt gcatgccttc catgacccgg
     6181 tactacgagg actcctttgg caaggcgtat gcccagtacg atgtcagcca ggcgaacagc
     6241 ctgctcgacc agatggggct tgccaagaag gacggcgacg ggttccgcct gcggtcggac
     6301 ggaaagcggc tcagcgttct catcgagtat gcggacggtt tcagcgcgcc gatcttcgag
     6361 ctgaccaagg aatactggga gaaggtgggc gtcgaagtca cgctgaagcc cgagagcgca
     6421 aagctgatct tcgagcgcta tcccaccaac gtagtgcagg tcggcggctg gcaggatgac
     6481 cgcggcacca cggaggactc cctcatccag tacccaagcc ggtatgtgcc gtgcgggtgg
     6541 cccccgtaca acaacgaatg gcctctgtgg ctgcagtggg cgcagtcgaa cgggaagcag
     6601 ggagaggagc ccattgccga ggtgaagaaa aacctcgacg ccctgaggac catgggtacc
     6661 acgaccagtg acgccgaacg ggtagccgcg gcgaaacaga tcctgcgttc ccaggccgag
     6721 aatctctggg tgatcgggac ggtcgggtat ccgccgttca tccggatcgt gcgctcgacg
     6781 atccacaacg tccctacgga cggcttcatg gggacctggg gagacgaagc gtac
//
LOCUS       DASRZS010000249         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_139024, whole genome shotgun sequence.
ACCESSION   DASRZS010000249 DASRZS010000000
VERSION     DASRZS010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139024"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            657..1724
                     /locus_tag="VFH83_06240"
     CDS             657..1724
                     /locus_tag="VFH83_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HET6485998.1"
                     /translation="MSGAHAPHSEETALLFPTGGYLWPGMGADLTASAAREILGRFES
                     ALEPCGVRPGRLFRLMEGEGQARREWTGTDWTWIGDFPLSMVAQTALGVALARARIES
                     LGPPRALAGESMGELAAYCVAGVLTVEETALLTFRWARDLQIASDQLGLRMAVLEDVP
                     EPELESWLADIPARIVVADAPGLLVVALPRSRLSFLDVEARRRGGRILVSNNPCAAHD
                     PQLAAAPGIWTAHEEFLQGLDFRSPTIPLWSTLDPGEMLHDPKDLQANRRETTVRRVH
                     WGETLMRMASAGIRHFEQFGPSASGYPLKRLRRLETSLQHVRISLISTEASALHLRGR
                     PTPPGTMVPRSGMKSSRGSPA"
     gene            1866..>2343
                     /locus_tag="VFH83_06245"
     CDS             1866..>2343
                     /locus_tag="VFH83_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pepsin-like aspartyl protease"
                     /protein_id="HET6485999.1"
                     /translation="MAGKPIRIAISNVLLGSDYTGRLYIGSRQEEVNLLLDTGSSTLA
                     VEHRSYDPQKDTKATVTDMVQEVQYGSGAWIGSVVKTDVAAGAGGETVDLPGVALAVA
                     YHESANMFGRSQGILGLAYQSLDDALVLTRPTVPPTYTPNEIRSGRRTFIEPYFTQL"
BASE COUNT          376 a          771 c          792 g          404 t
ORIGIN      
        1 tctcggtcca gtcggcccgg gcaaatcggg ccagctcctg gggaaagcga tcggcgttca
       61 tcgactcctc cggcgatgga ctctgcggtt gccaacgggc aagggaagcc aggaggtccg
      121 gcggtcacgg ccgaactgcc atcttgacgg aatcggagcc ggatgggatc ccggagttgg
      181 ccgcgcggtg aacgctggcc tgggacggac gcgtccaggg cggagtctcc cggctcccgc
      241 cgatcgccag accgatcccc agacagagac ggattcgctt cctatcaact tcctctcggg
      301 gtgtttcctc gattgggagc tggaacgccg atccggaccc tgggtgggcg gctgccgacc
      361 tctccgatgc cttcatttct tcgccgctgt cattatagcg cataatatta tacataagcg
      421 tcaatcgtga tccgtcatgg cccccaccgt cgcctcggcc gcgattcggc ttgattccag
      481 cgcccgtgcc tcccggctgc cctcggcggt tcgcttctgc cggaggagcg gagttgatcg
      541 tgcttgtctc gctgatcgct tggctcccgc gtggcgcgag gttagagtct ctccgaaggc
      601 gcgccatcga ccggaatgcg gcgccgggtg gaggggacgg cgggagagcc tggatcatgt
      661 cgggagctca tgctccacac tccgaagaga cggcgctgct ctttcccacc ggcggatacc
      721 tctggccggg catgggcgcg gacctgactg cctccgcggc ccgcgagatc ctgggccgat
      781 tcgaatccgc gctcgagccg tgcggcgtgc ggccgggccg gctcttccgc ctgatggaag
      841 gcgaaggcca ggcgcgacgc gaatggacag ggacggactg gacctggatt ggcgactttc
      901 cgctgagcat ggtggcccag accgcgctgg gggtggccct ggcccgcgct cggatcgaga
      961 gcctcggtcc tccgcgtgcg ttggcggggg agagcatggg cgagctggcg gcctattgcg
     1021 tggccggggt cctcaccgtg gaggagaccg cgctgctgac cttccgctgg gccagggatc
     1081 tgcagatcgc ctccgaccaa ctgggattga ggatggcggt gctggaggac gtcccggagc
     1141 cggagctgga atcctggctg gccgacatcc cggcgcggat cgtcgtggcc gacgctccgg
     1201 ggcttctggt cgtggcgctg ccccggtcac gcctgtcgtt cctggatgtg gaggcccggc
     1261 ggcgcggcgg gaggattctc gtctccaaca atccctgcgc cgcccacgat ccgcaactgg
     1321 ccgcggctcc cggaatctgg acggcgcacg aggaatttct tcaaggcctc gatttccgtt
     1381 caccgaccat cccgctctgg tccacgctcg atccgggaga gatgctgcac gatccgaagg
     1441 atctgcaggc caaccggcgt gagaccaccg ttcggcgggt tcactgggga gagacgctga
     1501 tgcgaatggc gagcgcggga atccggcact tcgagcagtt cggtccctcg gccagcggat
     1561 acccgttgaa acggctccgc cgcctggaaa cgtctctcca gcacgtgcgg atctcgttga
     1621 tctcgacgga agcatccgcc ctccaccttc gcggcaggcc cacgccgccc ggaaccatgg
     1681 ttccgcgatc tgggatgaag agctcgcgcg gatctcctgc ctgaccggcc tcgaaacctg
     1741 ttgcacggcc ccactcgctg cgtcagggtc tggcaaggct cctcggcgtc cgaatcgtga
     1801 accggataga cgtgaccgcg aggggccatc gcacggcaag gaacatttcc gaaggaggag
     1861 acgtcatggc tgggaagccc atccgcatcg ccatcagcaa cgtgctgctc ggcagcgatt
     1921 acacgggtag gttatacatc ggctcgcggc aggaggaggt caatctcctg ctggacaccg
     1981 gcagcagcac gctggcggtg gaacatcgca gctacgatcc gcagaaggat acgaaggcga
     2041 ctgtcaccga catggtgcag gaggtgcaat acggcagcgg cgcctggatc ggctcggtgg
     2101 tcaagaccga tgtggccgcc ggcgccgggg gagagacggt ggatctgccg ggtgtggccc
     2161 tggccgtggc ctaccacgaa tcggccaaca tgttcggccg ttcccaggga atcctcggcc
     2221 tggcctacca gtccctggac gacgccctgg tcctgacccg gccgacggtg ccgcccacct
     2281 acaccccgaa cgagatccgc tccggacgcc gcaccttcat cgagccctac ttcacccagc
     2341 tcg
//
LOCUS       DASRZS010000250         3114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4373599, whole genome shotgun sequence.
ACCESSION   DASRZS010000250 DASRZS010000000
VERSION     DASRZS010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4373599"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFH83_06250"
     CDS             <1..301
                     /locus_tag="VFH83_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486000.1"
                     /translation="QRYLKTMRTIHTVVMKQLGTVAQELGLQVLLLKDQHSRPDARAY
                     LKLLASFLHETVYSGLSVIVGFDYRAATLAFQAGAEKLLASMEIRPASPGATLQP"
     gene            complement(292..972)
                     /locus_tag="VFH83_06255"
     CDS             complement(292..972)
                     /locus_tag="VFH83_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET6486001.1"
                     /translation="MLSIYDRARRTAKRSIAASQDLRISVALDRPVGRPHTTAVKDGR
                     TLDCVVPAAGRSQRMGTWKPLLTFGSATIIQTVVARAASACRRVIVVAGYRGDELARL
                     LGGTSGVEVVQNPEWELGVFSSIRCGAAEVGSERFYITLGDMPWIRPEVYAVLEEPPS
                     ADAVFPVFGGRRGHPVLVSQSVRQAVLETDPTVPTMRQVLARFAAREVEWTDDSILRD
                     IDTPEDYG"
     gene            1029..2237
                     /locus_tag="VFH83_06260"
     CDS             1029..2237
                     /locus_tag="VFH83_06260"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008487810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HET6486002.1"
                     /translation="MSRYSQEFIRAIPKTDLHVHLDGSLRLGSLLEMARERRVKLPSQ
                     TEEGLDELVFKSSYANLNEYLECFTWTLKVLQDAEGLERSAYELAKDNQAEGVRYLEV
                     RFAPQLHMSSQLSFDAVVGAVDRGLRRARDEANRALPADEPAFDYGIIVCAMRSFTAE
                     FSDYYHNLCRVLSYSSQAEIIRAASLELARAAVQLRKESNLQVVGFDLAGAEHGFPAS
                     AHEESYEYVHRNFMKKTVHAGEAYGPESIFQAIAKLHADRIGHGLHMFDAEMIYSGSV
                     QDKRAYVENLVNYIAENRTTIEVCLTSNLQTSPSLRSVSDHSVREMLRNKLSLTFCTD
                     NRLVSHTTVSRELSLALENFSIPPDALKDIIIYGFKRSFYYHPYAMKRKWVRQVIDYY
                     ERLERTYGVA"
     gene            complement(2285..2452)
                     /locus_tag="VFH83_06265"
     CDS             complement(2285..2452)
                     /locus_tag="VFH83_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486003.1"
                     /translation="MAELYTAGKLAESLGISAGKVKKLIEELKIKEDSMKGVCKYYGE
                     AALKKLKAAAK"
     gene            complement(2468..2941)
                     /locus_tag="VFH83_06270"
     CDS             complement(2468..2941)
                     /locus_tag="VFH83_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486004.1"
                     /translation="MRLWQRRDREEDPQEFWKATAEKRGGAVGFTTFATLLGSPADRY
                     RGLPGLLYTIGDSVWFEDFERDNWFSRIVTTKTKFEKTEMSFQRQEVRSARLVSRNAA
                     LRCIAGGLEPDRIPTLSRLGKLLHSPAVQVRMNDGSSLFLEVMRAPEFVALFASP"
BASE COUNT          535 a         1084 c          979 g          516 t
ORIGIN      
        1 tcagcgctac ctcaagacca tgcggacgat ccataccgtg gtcatgaaac agctcgggac
       61 cgtcgcgcag gagctggggc tgcaggtcct ccttctgaag gaccagcact cccgtccgga
      121 tgcgcgggcc tatctgaagc tccttgcctc gttcctgcac gagacggtct acagcggtct
      181 cagcgtgatc gtaggattcg actaccgggc cgccacgctg gcgttccagg ccggcgcgga
      241 gaagctcttg gcctcgatgg agatccgccc ggccagcccc ggcgccacgc ttcagccgta
      301 gtcctccggc gtatcgatgt cccgtaggat cgaatcatcc gtccactcga cctcccgcgc
      361 ggcaaaccgg gccaggacct gacgcatggt cggcaccgtg gggtcggtct ccaggaccgc
      421 ctggcgcacc gactggctca ccaggaccgg gtggccacgc cggccgccga acaccgggaa
      481 caccgcatcc gccgagggag gctcctccag aacggcgtac acctcggggc gtatccaggg
      541 catgtccccc agcgtgatgt agaaccgttc gctgcccacc tcggcggcgc cgcatcggat
      601 ggaggagaac acgccgagct cccattcggg attctggacc acctcgaccc cgctcgtgcc
      661 cccgaggagg cgcgccagct cgtctccccg atagccggcg accacgatca ctcggcggca
      721 cgcgctggcc gcccgtgcga cgacggtctg gatgatcgtg gcgctcccga acgtcaggag
      781 cggcttccac gtccccatcc gctgcgagcg gccggcggcc ggaaccacgc agtccagggt
      841 gcgcccgtct ttcacggcgg tagtgtgcgg gcgccccacg gggcggtcaa gggcgacgga
      901 gatccgcagg tcctggctcg cggcgatgga ccgtttggcc gtccggcggg cgcggtcata
      961 gatcgaaagc aaggacccaa tctcgccttg accgctgcga tggcgagcgg tatcgtcacg
     1021 gcaaacccat gagccgatac agtcaggagt tcatccgggc aatccccaag acggacctgc
     1081 acgtccacct ggacgggtcc ctccgtttgg ggagcctgct ggagatggcg cgggagcggc
     1141 gggtcaagct gccctcgcag accgaagagg gtctcgacga gctggtgttc aagagctcgt
     1201 acgcgaacct gaacgagtac ctggaatgct tcacgtggac cctgaaggtc ctccaggacg
     1261 cggaggggtt ggagcgctcc gcctacgagc tggccaagga caaccaggcc gagggcgtgc
     1321 gctacctcga ggtccggttc gctccgcagc tccacatgtc atcccagcta tccttcgacg
     1381 cggtggtggg cgcggtggac cgggggctgc gccgggcccg ggacgaggcc aaccgcgcgc
     1441 tccccgccga cgagccggcg ttcgactacg ggatcatcgt ctgcgccatg cgctccttca
     1501 cggcggagtt ctccgactac taccacaatc tctgtcgggt gctgtcgtac tccagccagg
     1561 cggagatcat ccgcgccgcc agcctggagc tggcgcgggc cgccgtccag ctccgcaagg
     1621 agagcaacct gcaggtcgtt gggttcgacc tggccggggc cgagcacgga ttcccggcga
     1681 gcgcgcacga ggaatcctac gagtacgtcc atcggaactt catgaagaag acggttcacg
     1741 ccggggaagc gtacgggccg gagtccatct tccaggccat cgccaagctc cacgcggacc
     1801 gcatcggcca cggcctgcac atgttcgacg cggagatgat ctactccgga agcgtccagg
     1861 acaaacgggc ctacgtcgag aacctggtca actacatcgc ggagaaccgg accacgatcg
     1921 aggtctgcct gacgagcaac ctgcagacct caccgtccct gcgctccgtg agcgatcatt
     1981 ccgtgcggga gatgctccgc aacaagctgt ccctcacgtt ctgcacggac aaccgcctgg
     2041 tgtcccatac cacggtgagc cgcgagctct ccctggcgct ggagaacttc tccatcccgc
     2101 cggatgccct gaaggacatc atcatctacg ggttcaagcg gtccttctac taccatccct
     2161 atgcgatgaa gcggaagtgg gttcggcagg tgatcgacta ctacgagcgg ctcgagcgca
     2221 cctacggcgt ggcctgaggc cgggaccttc cgcccggggg cctcagaccc gccggagccc
     2281 cgcgctactt cgcggccgct ttcagcttct tgagcgccgc ttctccgtag tacttgcaga
     2341 ctcccttcat ggaatcctct ttgatcttca gctcctcgat gagcttcttc accttacccg
     2401 cggatatgcc gaggctttcc gcgagctttc ccgccgtgta cagctctgcc atggttccct
     2461 ccggatgtta tggtgatgcg aagagggcca cgaactccgg ggccctcatt acctccagga
     2521 agagcgacga gccgtcgttc atccgcacct ggaccgccgg gctgtgcagg agcttgccaa
     2581 gcctcgagag cgttgggatc cgatccggct ccaggccgcc cgctatgcac cgaagcgccg
     2641 cgtttcggga caccagccgc gcggagcgaa cctcctggcg ctggaagctc atctcggtct
     2701 tctcgaactt ggtcttggtg gtgacgatac gggagaacca gttgtccctc tcgaagtcct
     2761 cgaaccagac ggagtcgccg atggtgtaga ggaggccggg caacccccgg taccggtccg
     2821 cgggcgagcc cagcaaggtc gcaaaggtcg tgaaccccac ggcaccgccg cgcttttccg
     2881 cggtagcctt ccagaactcc tgggggtcct cctcccggtc ccgccgctgc cacagccgca
     2941 tggcccacca tagcgcggcg ggagcaaaga ggcaaggcaa aacgcaccgg cggacggcgc
     3001 atgcgacgcc agcgcattgt gacccgcagc cggccgttgt atcatccgcc atgcccggcc
     3061 ttctctcctg cccggtggag acgtacctgc ccctgctcct caagctctgg cgcg
//
LOCUS       DASRZS010000251         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_140049, whole genome shotgun sequence.
ACCESSION   DASRZS010000251 DASRZS010000000
VERSION     DASRZS010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140049"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..1051
                     /gene="cysA"
                     /locus_tag="VFH83_06275"
     CDS             5..1051
                     /gene="cysA"
                     /locus_tag="VFH83_06275"
                     /EC_number="7.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HET6486005.1"
                     /translation="MDLDVARGELVALLGPSGCGKTTLLRVIAGLEIPDGGRVELDGE
                     DATRADARHREVGFVFQHYALFRHLSVFENVAFGLRVRPRATRPPRAEVARRAQRLLE
                     LVQMEWAAHRFPTQLSGGQRQRVALARALAVEPRVLLLDEPFSALDAKVRLELRRWLR
                     RLHDEIQVTTVFVTHDQEEALELADRIVIINQGEIVQAGKPEEVYDHPANAFVWSFLG
                     NVNVFHGRLNGSEARFGALRLSLNDRAPEDRREVHGFIRPHDIRVSARRESPHDLPAT
                     LVTSRMSGKMQMVEIRMDGVEQALEALVERKVFQSWNLAPGGPLWVRLERISLHPTKE
                     DGASSPDESFDWVI"
     gene            complement(1094..2152)
                     /locus_tag="VFH83_06280"
     CDS             complement(1094..2152)
                     /locus_tag="VFH83_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6486006.1"
                     /translation="MDVNYSIKDIARLAGVSVATVARALKGKPDINRETSDRVLAIAK
                     RYNYQPNLLARGLVTQKTFTVGIIVPAIDNPFFPALIRGIEATLWESRYNVILSDTGY
                     DPAKEEAALREFRSRRVDGVIISPINKIAVSPWLAEVAASAFPMVFLTRTDYPGANAV
                     FADDRGGAHAITRHLLSLGYRRIVYLGNRMSAWANEERITGYTNALSEAGVGLEGTLI
                     RLSEGNSSESACACTREVLAEGLRADAIFSFDDIMAIGVKRALAEAGLQVPRDIALVG
                     FDNIDIISLPEIDLTTVDIPKRELGIEAARLLIDLIGRTRDGDRTDRRGAVAPKTVIL
                     DTTLVIRGSCGSRLRRGS"
     gene            complement(2152..3030)
                     /locus_tag="VFH83_06285"
     CDS             complement(2152..3030)
                     /locus_tag="VFH83_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020619327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6486007.1"
                     /translation="MRIQKGEMPFHVVNTSLLILLALTCVIPFIHVLALSFSSGLAAD
                     KGIVGLWPVYPTVAAYQYTFQDTGFIRAFGITVLRVILGVTIAMILNVLTAYPLSKTA
                     RQFPGRSLLSWFFVVTMLVNGGLIPTYLIVSATRLRNTIWALVVPGAVWAFNVMLLLN
                     FFRQLPKELEESAYLDGAGPWRILFQIYIPLSTAVLATLVIWTTVGHWNEWFAGLIYM
                     DSIRNYPLQTYLQGIIVEPRFDLLDISQIELLSRLSRRTFNAAQIIIATVPVITVYPF
                     LQRYFVKGMQLGGLKG"
     gene            complement(3042..>3300)
                     /locus_tag="VFH83_06290"
     CDS             complement(3042..>3300)
                     /locus_tag="VFH83_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6486008.1"
                     /translation="PVIVLVAILNLGNVLNAGFDQIFNLYNALVYSTGDILETLTYRL
                     GIGSFQYDLATAVGMFKSGVSLVLVSTSYFIAWKFADYRIF"
BASE COUNT          583 a          984 c         1173 g          560 t
ORIGIN      
        1 agggatcgac ctggacgtgg cgcgtgggga gctggtggcg ctcctggggc cgtcggggtg
       61 cggcaagacc acgctgctgc gggtcatcgc cgggctggag atcccggacg gcggacgcgt
      121 cgagctggac ggggaggacg caacccgggc ggatgcccgt caccgcgagg tgggcttcgt
      181 cttccagcac tatgcgctgt tccggcacct gagcgtgttc gagaacgttg ccttcggtct
      241 gcgcgtccgc ccccgcgcca cgcggccgcc tcgcgcggaa gtggcgcgcc gagcgcagcg
      301 gctcctggag ctggtgcaga tggaatgggc ggcgcatcgg ttccccactc agctatccgg
      361 cggacagcgc cagagggtcg ccctggcccg cgcgcttgcg gtcgagccga gggtcctgct
      421 gctcgacgag ccgttctcgg ccctggacgc caaggtgcgt ctggagctgc gccgttggct
      481 ccgtcgactc catgacgaga tccaggtcac cacggtcttc gtcacccacg atcaggagga
      541 agccctggag ctggcggacc ggatcgtcat catcaaccag ggggagatcg tgcaggccgg
      601 caaaccggag gaggtgtacg atcatccggc caacgcgttc gtgtggagct tcctgggcaa
      661 cgtgaacgtc ttccacggcc ggctgaacgg atccgaagcc cggttcggcg ctctgcggct
      721 gtctctcaac gatcgcgccc cagaagacag gcgcgaggtc catgggttca tccgacctca
      781 tgatatccgt gtctcggcgc gccgcgagag ccctcacgac cttccggcga cgctcgtgac
      841 cagtcgcatg agcgggaaga tgcagatggt ggagatccgg atggacggcg tcgagcaggc
      901 cctggaggcc ctcgtcgagc ggaaggtgtt tcagtcctgg aacctggcgc ccggaggacc
      961 gctctgggtg cgcctggagc ggatatcgct gcatcccacg aaggaggacg gggcgtcgtc
     1021 gcccgacgag tcgtttgact gggtgatctg aaaggaggcg cgacgtccct tcagtcgcgt
     1081 atcgggttcg gcatcaactt ccgcggcgca gtcgcgagcc gcacgagccc cggatgacga
     1141 gcgtggtgtc gagaatgacc gtcttcggcg caacggcgcc gcgccggtcc gtccggtcgc
     1201 catcccgagt tcgcccgatc aggtcgatga ggagccgcgc cgcctcgatg cccagctccc
     1261 gcttcggaat gtccaccgtg gtcaggtcga tctcgggcag cgagatgatg tcgatgttgt
     1321 cgaatccgac gagagcgatg tcgcgcggga cctgcagccc ggcctccgcg agggcgcgtt
     1381 tgacgccgat ggccatgatg tcgtcgaagg agaatatcgc gtccgcccgg aggccctccg
     1441 cgagcacttc ccgcgtgcac gcgcaggcgc tctcgctgct gttgccctcc gacagccgga
     1501 tcaacgtgcc ctcgagaccg acgcccgcct ccgaaagcgc gttggtgtag ccggtgatcc
     1561 gctcctcgtt ggcccaggcg ctcattcggt tgcccaggta gacaatgcgg cggtacccca
     1621 gggagaggag gtggcgggta atggcgtgcg cgcccccgcg gtcgtcggcg aaaaccgcgt
     1681 tggcgccggg gtagtccgtc ctcgtcagga agaccatcgg gaaggcgctg gcggccactt
     1741 cggccagcca cggactcacg gcgatcttgt tgatgggtga gatgatcacc ccatccacgc
     1801 ggcgcgagcg gaactcgcgg agcgcagcct cttccttggc cggatcgtag ccggtgtccg
     1861 agaggatgac gttgtagcgg gattcccaga gggttgcttc gatccccctg atgagcgcgg
     1921 ggaagaaggg attgtcgatg gcggggacga tgatcccgac ggtgaaggtc ttctgggtca
     1981 ccagcccgcg ggccaggagg ttgggctggt agttgtacct cttcgcgatg gcgagaaccc
     2041 gatcgctggt ctcgcggttg atgtccggct tgcctttgag ggctcgggcg accgtggcca
     2101 cggagacgcc ggccaaccgg gcgatgtcct tgatggaata gttgacgtcc atcacccctt
     2161 cagacccccg agctgcattc ccttcacgaa gtatctctgg aggaaggggt agacggtgat
     2221 cacggggacg gtggcgatga tgatctgcgc cgcgttgaag gtgcgccggg agagccggga
     2281 gagcagctcg atctggctga tgtcgagcag gtcgaagcgc ggctcgacga tgattccctg
     2341 gaggtacgtc tggagtgggt agttccggat cgagtccatg tagatgagcc cggcgaacca
     2401 ctcgttccag tgcccgacgg tcgtccagat caccagcgtg gccaacaccg ccgtggagag
     2461 cgggatgtag atctggaaca ggatgcgcca gggaccggcg ccgtcgaggt atgcggactc
     2521 ctcaagctct ttggggagct ggcggaagaa gttcaagagg agcatgacgt tgaaggccca
     2581 cacggctccg ggaacgacga gggcccagat cgtgttgcgc agacgcgtgg cgctcacgat
     2641 cagataggtg gggatcagcc cgccgttgac gagcatcgtg accacgaaga accaggagag
     2701 gaggctccga cccgggaatt gccgggcggt cttggagagc ggataggcgg tcagaacgtt
     2761 caggatcatg gcaatggtca cgccgaggat gacgcgcagg accgtgatgc cgaaggcgcg
     2821 gatgaagccc gtatcctgga acgtatactg gtaggccgcc acggtggggt agaccggcca
     2881 cagaccgaca atccccttgt ccgccgccaa tcccgatgag aacgagaggg ccagcacgtg
     2941 gatgaacggg ataacgcagg tgagggccag gaggatgagc aggctcgtgt tgacgacgtg
     3001 gaacggcatt tcgcctttct ggattctcat ggccttctct ctcagaagat ccggtagtcg
     3061 gcgaacttcc acgcgatgaa gtacgaggtg gagaccagca cgagggagac accggacttg
     3121 aacatcccta cggcggtggc caggtcgtac tggaagctcc cgatcccgag ccgataggtg
     3181 agggtctcca ggatgtcacc cgtcgagtag accagggcgt tgtacaggtt gaagatctgg
     3241 tcgaagccgg cgttgaggac gttgcccagg ttcaggatgg ccacgagcac gatcaccggc
//
LOCUS       DASRZS010000252         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2451269, whole genome shotgun sequence.
ACCESSION   DASRZS010000252 DASRZS010000000
VERSION     DASRZS010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2451269"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VFH83_06295"
     CDS             <1..1155
                     /locus_tag="VFH83_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET6486009.1"
                     /translation="LPPSELKVKADHLRAELAKAGASGVLIVQEGAMRWLTGTRHQIS
                     DVAADAASPVQALVLSKGSGLDITFLTTRIEMPRVQDQLGPIYERAEGIKAAYSTDRP
                     SLPVGVSAPGSPGYADLIGGIVMPLVGGLEGDPYARLSWLIAATTALLAKSAHEIEPG
                     MNGAQVRARVLANLLEHDIESNLVLVALAGQEGHLHPLYDARYRIERDGWVKLVAGAR
                     LAEMIVSATVQVKFGKPPSKEAAEGYRALQEGTVEYADCYRAGAVEGDIYTEVGRRFA
                     DLERAHALPGFAAAAYAHHMGGPTSPIGNRDYLVEKGGKHVIRAWTQFAINPVEPKAN
                     TKVELQGIVMPQGAPHVPDLSTFTPPALLTFREVVSKGGARATVADILQR"
     gene            complement(1152..>3628)
                     /gene="clpB"
                     /locus_tag="VFH83_06300"
     CDS             complement(1152..>3628)
                     /gene="clpB"
                     /locus_tag="VFH83_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HET6486010.1"
                     /translation="EQKDGVVPPILERIGADPSEVSSAIRRLLADQPTVRGDAAQRSM
                     SARLANALNKAEQEAEALKDEYTSTEHLLLAILETDGEGRDLLRARGVTREAVLEALK
                     SVRGNQRVTDQNPEARYQSLEKFTRDLTELARKEKLDPVIGRDEEIRRVMQVLARRTK
                     NNPVLIGEPGVGKTAIVEGLARRIVSGDVPDSLKDKKLLALDLGALVAGTKFRGEFEE
                     RLKAVIKEITQSEGSIILFIDELHTLVGAGAAEGAMDASNLLKPALARGELRAIGATT
                     LDEYRKRIEKDPALERRFQPIFTSQPTVEDTIAILRGLKERYEVHHGVRIRDDAIIAA
                     AVLSDRYITNRFLPDKAIDLIDEAASRLKMEIESQPTELDRAERRILQLNIEKQALSK
                     ESDPASKERLGALEKELADLGTKRDAMKLQWGTEKGIIEEIRKRKAELEELSIEETRY
                     EREGNLSKAAEIKHGRLPETQRRLEEKSKELEALHGETRLLREEVSEEDIARIVSNWT
                     GIPVTKMLASEKVKLLKLEDILSKRVVGQQPAIVAVSDAIRRNKSGLSDAGRPVGTFL
                     FLGPTGVGKTELAKTLADFLFSDEKAVTRIDMSEYMEKHTVSRLIGAPPGYVGYEEGG
                     QLTEAVRRRPYSVILFDEIEKAHPDVFNVLLQLLDEGRLTDGQGRTVDFRNALIIMTS
                     NIGGDLIQNAAKIEDIRDAISALLKATFKPEFLNRIDETIIFNRLGKDEILKIVDIQL
                     DLLKNRLKDKKVTLEATPKAKAFLAEAGFDPQFGARPLKRTIQSLVQNPLAKRILEGQ
                     IAEGDAVTVDRGADGIVFKKKSGAAP"
BASE COUNT          523 a         1235 c         1178 g          692 t
ORIGIN      
        1 ctcccgccgt cggagctgaa ggtcaaggcg gaccacctgc gcgctgagct ggcaaaggcg
       61 ggcgcttccg gcgtgctgat cgtccaggag ggagccatgc gctggctcac gggaacccgg
      121 caccagatca gcgatgtcgc cgcggacgcc gcaagcccgg tgcaggccct tgtcctgtcc
      181 aaggggagcg ggctggacat cacgttcctt acgacccgga tcgagatgcc tcgcgtgcag
      241 gatcagctcg gaccgatcta cgagcgcgcg gaaggcatca aggctgccta ctccacggac
      301 cggccgtccc tccccgtcgg cgtttctgcg cccggttccc cgggctacgc ggacctcatc
      361 ggggggatcg tgatgccgct ggttggtggc ctggaaggag atccgtatgc gcggctctcc
      421 tggctgatcg cggcgaccac cgcgcttctg gccaagagcg cgcacgagat cgagccgggc
      481 atgaacgggg cgcaggtccg tgcccgcgtg ctggccaacc tgctggagca cgacatcgag
      541 tccaacctgg tgctggtggc cctggccgga caggaggggc atctccatcc tctgtatgac
      601 gcgcgctatc ggatcgagcg cgacggctgg gtgaagctgg tggccggcgc gcgtctggcg
      661 gagatgatcg tatccgcgac ggtccaggtg aagttcggca agccgccgtc caaggaggcc
      721 gcggaagggt atcgggccct ccaggagggg accgtcgagt acgccgactg ctaccgggcc
      781 ggtgcggtcg aaggagacat ctacaccgag gtgggccgca ggttcgcgga cctggagcgc
      841 gcgcatgcgc tgccgggctt tgccgcggcc gcctacgctc accacatggg gggacccacg
      901 agccccatcg gcaaccgcga ctacctggtg gagaagggcg gcaagcacgt catccgggcg
      961 tggacccagt tcgccatcaa ccccgtcgag ccgaaggcca acaccaaggt cgagctccag
     1021 gggatcgtga tgccgcaggg cgcgccccac gtgccggacc tctccacctt cacgccgccg
     1081 gcgctcctca cgttccgtga ggtggtttcc aagggcggcg cgcgcgcgac ggtggcggac
     1141 atcctccagc gctaaggggc cgctcccgac ttcttcttga acacgatccc gtccgcgccg
     1201 cggtcgacgg tcaccgcatc gccctccgcg atctggccct ccaggatgcg cttggccagg
     1261 gggttctgca cgaggctctg gatggtccgc ttcaggggcc gggcgccgaa ctgaggatcg
     1321 aagccggctt ccgccaggaa cgcctttgcc ttcggcgttg cctccagcgt caccttcttg
     1381 tccttgagcc ggttcttcag aaggtcgagc tggatgtcga cgatcttgag gatctcgtcc
     1441 ttgcccagtc ggttgaagat gatggtctca tcgatgcggt tcaggaactc gggcttgaag
     1501 gtggccttga gaagcgccga gatggcgtcc cggatgtcct cgatcttggc ggcgttctgg
     1561 atcaggtccc cgccgatgtt gctcgtcatg atgatcaggg cgtttcggaa atccaccgtg
     1621 cggccctgcc catcggtgag cctgccttca tccaggagct gcagaaggac gttgaacacg
     1681 tccgggtggg ccttctcgat ctcatcgaac aggatcacgg agtagggcct tcgccggacc
     1741 gcttcggtga gctggcctcc ctcctcatac cccacgtacc cggggggcgc gccgatcagc
     1801 cgtgagacgg tgtgcttctc catgtactcg ctcatgtcga tgcgggtcac cgccttctca
     1861 tcgctgaaga ggaagtccgc cagggtcttg gcgagctcgg tcttgcccac ccccgtggga
     1921 cccaggaaca ggaaggtgcc gaccgggcgg ccagcgtccg acagcccgct cttgttccgc
     1981 cggatggcat cggaaaccgc gacgatggcc ggctgctgtc ccaccacgcg cttggacagg
     2041 atgtcctcca gcttgaggag cttcaccttt tccgatgcca gcatcttggt gacggggatg
     2101 cccgtccagt tggagacgat ccgggcgatg tcctcctccg acacttcctc tctcaggagc
     2161 cgggtctcac cgtgcaaggc ttccagctcc ttgctcttct cttccagcct gcgctgcgtc
     2221 tccggcagcc tcccgtgctt gatctccgcc gccttgctga ggttgccctc ccgctcgtag
     2281 cgcgtctcct cgatcgaaag ctcctccagc tccgccttgc gctttcggat ctcctcgatg
     2341 atccctttct cggtgcccca ctggagcttc atggcgtccc gtttggtgcc caggtccgca
     2401 agctccttct ccagcgcgcc gagccgttcc ttcgaggccg ggtcgctctc cttggagagc
     2461 gcctgcttct caatgttgag ctgcaggatc ctgcgctccg cgcggtccag ctccgtgggc
     2521 tggctctcga tctccatctt gagccggctc gccgcttcgt cgatcaggtc gatggccttg
     2581 tcgggcagga accggttggt gatgtagcgg tcggagagga cggccgcggc gatgatggca
     2641 tcgtcccgga tccgcacccc gtggtgcacc tcgtagcgct ccttgagccc gcggaggatg
     2701 gcgatggtgt cctccacggt gggctgggag gtgaagatgg gctggaacct ccgctccagc
     2761 gccgggtcct tctcgatcct ctttcggtac tcgtccagcg tggtcgcgcc aatggccctg
     2821 agctcaccgc gcgccagcgc cggtttcagg aggttggacg cgtccatggc cccttccgcg
     2881 gcgccggcac ccaccagggt gtgaagctca tcgatgaaga ggatgatgct cccctcgctc
     2941 tgggtgatct ccttgatcac cgctttgagg cgctcctcga actctccgcg gaacttcgtc
     3001 ccggccacca gggcgcccag gtccagggca aggagcttct tgtccttcag ggagtccggc
     3061 acgtccccgg agacaatgcg tcgggccagg ccttccacga tggcggtctt tccaacgccc
     3121 ggctccccga tcaacaccgg gttgttcttg gtcctgcggg ccagcacctg catgacccgg
     3181 cgtatctcct cgtccctgcc gatgaccggg tccagcttct cctttcgggc aagctcggtg
     3241 aggtcccggg tgaacttctc cagggactgg tagcgcgcct cggggttctg gtccgtgacc
     3301 cgctggtttc cacggacgct cttgagcgct tccagaaccg cctcccgggt cacgccgcgg
     3361 gccctcaaga ggtcacggcc ctccccgtcg gtttccagga tggccaggag caggtgctcg
     3421 gtcgaggtgt actcgtcctt gagcgcctcg gcctcctgct ccgccttgtt cagcgcattg
     3481 gcaagacgcg ccgacatcga tcgctgcgcc gcgtccccac gcacggtggg ctgatccgcc
     3541 agcaggcggc ggatggcgga cgagacctcg gacgggtccg cgccaatgcg ctcgaggatc
     3601 ggcggaacca cgccgtcctt ctgttcca
//
LOCUS       DASRZS010000253         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_141113, whole genome shotgun sequence.
ACCESSION   DASRZS010000253 DASRZS010000000
VERSION     DASRZS010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141113"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VFH83_06305"
     CDS             <1..1078
                     /locus_tag="VFH83_06305"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6486011.1"
                     /translation="TLQASLNNNFLVGEIGISGFQAGLLECVRETCGILALGLFAIIA
                     GLAEPVIATFALLFVTAGLTSYSFAPSYGFVMLMSLTWSMGFHVWTPLPNSMTLALAE
                     PGRAGARLGRVGAMGALGSGIGLVAAFALTEFGVRIRPLYILAGGATLLAAAACLRIP
                     RHLKAPGPSFVYRRRYLRYYMLNFLEGWRKQIAICFGGFLLVRIYHAPLLEILILNAA
                     IQTIGYFLSPRVGKLIDRIGERSILTFYYVTVTILFLGYAFIKDKYLLYAIYILDNAT
                     FVFNTGLTTYVNKIAPKSEHRPTLSMGVAANHVAAVCMPLLGGILWTTLGYQWAFLVG
                     LPAAAASIAIVSGLPGGTRQVTST"
     gene            1152..>2212
                     /locus_tag="VFH83_06310"
     CDS             1152..>2212
                     /locus_tag="VFH83_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6486012.1"
                     /translation="MTSRERVLRAVNFQTPDRVPIDLGGTKSSGIAASTYDRVKKLVG
                     IASPTRVWDARFMISMVEPEVLTRFHADVLPVDLTAIESARHPDREWVPRTLFDGTKT
                     LLPPGTSIQEDTEGNWVLLDADGAPTSYRLPRGGYYFDDLSFNKGDRIDPSLFQPQTD
                     IDDVSLRLLSDNARRLYRETDYALLGWGYGVCFLGLSLVTDRKNNVTQGLPDQWMMML
                     MTEKETCHEMMGRSVDAAIQCLKLVKEAVGDTCFAWGIASDDSGTQRREFIHPDLWAE
                     MIKPHYARLCSWVHANTSMKTYFHCCGSIPNLIPHLIDAGVDILNPVQTSAAGMEPET
                     LKERFGGRIVFWGGGCDTQS"
BASE COUNT          362 a          736 c          721 g          393 t
ORIGIN      
        1 gaccttgcag gcgagcctga acaacaactt cctggtcgga gagatcggca tctcggggtt
       61 ccaggcgggg ctcctggagt gcgtgcggga gacctgcggg atcctggcgc tggggctttt
      121 cgccatcatc gccgggctgg cggaaccggt gatcgccacc ttcgccctgc tcttcgtgac
      181 ggcgggtctc acatcgtact ccttcgctcc cagctacggg ttcgtgatgc tcatgagcct
      241 gacctggagc atggggttcc acgtctggac gccgcttccc aactccatga cgctggcgct
      301 ggcggagccg ggccgcgcgg gcgcgcgcct gggaagggtc ggggccatgg gagcgctggg
      361 ctccgggatc gggctggtgg cagcctttgc cttgacggag ttcggtgtgc ggatccggcc
      421 gctgtacatc ctggccggcg gggcgacgct cctggccgcc gcggcctgtc tgcgcatacc
      481 gcggcacctc aaggcgccgg gacccagctt cgtgtaccga cgccgctacc tgcgctacta
      541 catgctgaac ttcctggaag gctggcgcaa gcagatcgcc atttgctttg gcgggttcct
      601 cctggtccgc atctaccacg cgccgctcct ggagatcctg atcctgaacg cggccatcca
      661 gaccatcggg tatttcctct ccccgcgcgt tgggaagctt atcgaccgga tcggggagcg
      721 gagcatcctt accttctact atgtgacggt gaccatcctg ttcctgggct acgccttcat
      781 caaggacaag tacctcctct acgcgatcta catcctggac aacgccacgt tcgtcttcaa
      841 taccggcctt accacctacg tgaacaagat cgcgcccaag agcgagcacc gacccactct
      901 gagcatgggg gttgcggcca accacgtggc cgccgtctgc atgccgctcc tcggcgggat
      961 cctttggacc acgctgggct accagtgggc attcctggtg ggtctgcccg ctgccgcggc
     1021 cagcatcgcc atcgtctccg ggctgccggg gggaaccagg caagtgacat cgacgtgatc
     1081 ccggagccgg ccccatgggg ccgagcagcc gagggaagcc gggcgcatcg gaaccggcgg
     1141 ggaggcagat tatgacgtct cgcgagcgcg ttctgagggc cgtcaacttc cagaccccgg
     1201 accgggtgcc catcgacctg ggtgggacca agtcatccgg gatcgccgcc agcacgtacg
     1261 accgggtgaa gaagctggta gggatcgcca gccccacccg ggtctgggac gcgcggttca
     1321 tgatctccat ggtggagccg gaggtcctca cccggtttca cgcggacgtc ctccccgtgg
     1381 acctcacggc aatcgagagc gcgcgccacc cggaccggga atgggtgccc cgcacgctct
     1441 tcgacggcac aaagaccctg ctcccgccgg ggacctccat ccaggaggac accgagggca
     1501 actgggtcct cctggacgcg gacggcgcgc cgacctccta tcgactcccg cgcggcgggt
     1561 actacttcga cgatctgtcg ttcaacaagg gcgatcggat cgacccgtcg ctgttccagc
     1621 cgcagacgga catcgacgac gtgtcgctgc gcctcctgtc cgacaacgcc cgccgcctgt
     1681 accgcgagac ggactacgcg ctcctggggt gggggtatgg ggtatgcttc ctgggtctga
     1741 gcctggtcac ggaccgcaag aacaacgtga ctcagggcct tccggaccag tggatgatga
     1801 tgctgatgac ggagaaggag acctgtcacg aaatgatggg tcgctccgtg gatgccgcca
     1861 tccagtgtct gaagctcgtg aaggaagcgg tgggggacac ctgcttcgcc tggggcatcg
     1921 catcggacga cagcggtacc cagcgccggg agttcatcca tcccgacctt tgggcggaga
     1981 tgatcaagcc gcactacgcg cggctgtgct cgtgggttca cgccaacacg tcgatgaaga
     2041 cctacttcca ctgctgcggc tccatcccca acctgatccc gcacctgatc gatgccggcg
     2101 tggatatcct caacccggtc cagacctccg cggcgggcat ggagcccgag acgctcaagg
     2161 agcggttcgg cgggcgcatc gtgttctggg gcggcggttg cgacacgcag tc
//
LOCUS       DASRZS010000254         3310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_141628, whole genome shotgun sequence.
ACCESSION   DASRZS010000254 DASRZS010000000
VERSION     DASRZS010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141628"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..1486
                     /locus_tag="VFH83_06315"
     CDS             5..1486
                     /locus_tag="VFH83_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET6486013.1"
                     /translation="MFDIYTRINSTYADLKALLTRIIESATELTEGEASSLMLVDPEN
                     NKLYFEIALGPKGPSAKQYSLNMGEGIAGWVAEKNTPLIVNDANSDPRYYPDIAQKIG
                     FPATSILAVPMRVKERMVGVIEILNKRGGKRFTEEDLQWLEVFATQAAIAVQNARSFE
                     RVRNELAVLQDKVQVGQGFHTLIGQSKAIQDKLEIARKAAQTDSSVLLLGESGVGKEL
                     FAEQIHLASPRAGRPLIRVNCAAIPESLLESELFGHVKGAFTDAIADRRGRFELADGG
                     TLFLDEIADMPLALQAKLLRVLQSKTFEKVGSSDSIRVDVRIIAATNHNLEREVTAGR
                     FRADLFYRLNVLPITIPPLRERREDVPLLAEFFLKKHCRETKKQLLGFSDDAMAQLLS
                     YSWPGNVRELENAVERAVVFTRDERIRADALTLSGGAGFVANGSGPKTLHEALTAFKK
                     QFIARALESNGWHQTRTAKELSIQRSYLSRLVRELNIMKERGV"
     gene            1492..2211
                     /locus_tag="VFH83_06320"
     CDS             1492..2211
                     /locus_tag="VFH83_06320"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6486014.1"
                     /translation="MQPVQQQAGKQAPPEHLGRRISIFLSHTIDRFRIALWVILIAAA
                     VFLVGYFIYTEVAKKLAADSTVLAESAQAAYDKWQAENDATKKAEDQKDLQSQLDRLI
                     GRYPRQYGGQRGLFLRANVEYETKGWDAAAKDYQLLARRFPNSYLAPISEFNAAIALE
                     EKGDVDGALALYKSIPQSYKSSTMAPRALFDAGRLEEQKNAWDEAQKEYAAIDTDYPQ
                     SDWNQLAKDRLIQLKVLGKIK"
     gene            2267..>3310
                     /locus_tag="VFH83_06325"
     CDS             2267..>3310
                     /locus_tag="VFH83_06325"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HET6486015.1"
                     /translation="MAQKKWTARFLETKYFGLVIGLVVFVLLFLLTFQTTIVGYVEQK
                     LLDFNFRVKSTVGSARVQEGVSVVQANPRISPDILIVGIDDKALARFGRWPFPRFRHA
                     DLINNLARIKNQDDRERALFVDVFFIEPSEVPEDDALLVSSIKSNGRVFLETVLTLDE
                     NPPGTDEEFFGRESLLESTYGTITNVRGDWLKTNTFFGVQSPLKPYSRATYGYGHANF
                     REDPDQVYRSQPLVVKLSQLEKEIPLDKLTVNEPIDRSHFERLAWIDKQNETHEVPYP
                     LTAGVLEGLNRQMTRNAPLKVVEASDTQAAASYYVVRKYRDTFLPSITLSLALEYMHK
                     KLSDIEVVMGQYIR"
BASE COUNT          697 a         1025 c         1026 g          562 t
ORIGIN      
        1 agtcctgttc gatatctaca cccggatcaa ttccacgtac gcggacctga aggcgctgct
       61 caccaggatc atcgagtccg ctacggagct gacggagggg gaggcatcct ccctcatgct
      121 ggtggatccg gagaacaaca agctctactt cgagatcgcg ctggggccca agggtcccag
      181 cgccaagcaa tactcgctca acatggggga agggattgcg ggctgggtag cagagaagaa
      241 caccccgctg atcgtgaatg acgccaacag tgacccgcgc tattaccccg acatcgccca
      301 gaagatcgga ttcccagcga cctccattct tgccgtcccc atgcgggtca aggagcggat
      361 ggtgggcgtg atcgagatcc tgaacaagcg aggcggtaag cgtttcacgg aagaagacct
      421 ccagtggctg gaggtctttg ccactcaggc cgccatcgcc gtccagaacg cgcggtcctt
      481 cgagcgggtg cgcaacgagc tcgcggtcct ccaggacaag gttcaggtcg gccaggggtt
      541 ccacacgctc atcgggcaga gcaaggccat tcaggacaag ctggagatag cccgtaaggc
      601 ggcgcagacg gactcttcgg ttcttcttct gggagaaagc ggggtaggga aggagctctt
      661 cgcggagcag atccacctgg cgagcccccg ggcgggtcgg cccctcatcc gcgtcaactg
      721 cgccgccatt ccggagagtc tcctggagag cgagctcttc ggccatgtga agggcgcctt
      781 cacggacgcc atcgcggacc gacgcggacg gttcgagctg gcggacgggg gcacactctt
      841 tctggacgag atcgcggaca tgcccctggc gctccaggcc aagcttcttc gggtgcttca
      901 gagcaagaca ttcgagaagg tcggctcgag cgactcaatc cgtgtggacg tgcggatcat
      961 cgcggccacc aaccacaacc tggaacgcga ggtgacggcg ggacggttcc gcgctgatct
     1021 cttctaccgc ctgaatgtcc tccccatcac cattccgccc cttcgggagc ggcgggagga
     1081 cgttcccctc cttgcggagt tctttctcaa gaagcactgt cgggagacca agaagcagct
     1141 cctgggattc agtgatgatg ccatggcgca gctcctgtcc tactcctggc ccgggaacgt
     1201 ccgcgaactg gagaacgccg tcgagcgagc ggtcgttttc acccgggacg agcgtatccg
     1261 cgcggatgct ctgaccctca gcggcggagc ggggttcgtg gccaacggca gcggccccaa
     1321 gacgctccac gaggctctta cggcgttcaa gaagcagttc attgcccggg ccctggagtc
     1381 caacggatgg caccagaccc ggaccgccaa ggagctctcg atccagcgca gttacctctc
     1441 acgcctcgtg agggaactca acataatgaa ggaaagaggg gtatagagcc tatgcagcca
     1501 gtgcagcagc aggcggggaa gcaggcgcct ccggaacacc tgggccgcag gatcagcatc
     1561 ttcctgtctc acacaatcga ccgattccgc atcgcccttt gggtgattct catcgcggcg
     1621 gcggtattcc tggtgggtta cttcatctac acggaagtgg cgaagaagct cgccgcggat
     1681 tctacggtgc tcgccgagag cgcccaggct gcctacgaca aatggcaagc cgagaacgac
     1741 gcgaccaaga aggcggagga ccagaaggac ctccagagcc agcttgaccg tctgatcggg
     1801 cgataccccc gccagtacgg cgggcagcgg gggctcttcc tccgggctaa cgtggagtac
     1861 gagaccaagg gatgggatgc cgcggcaaag gactaccaac tgctggcgcg acggttcccc
     1921 aacagctacc ttgcgccgat cagcgagttc aacgccgcca tcgcccttga ggagaaaggc
     1981 gacgtcgacg gagcgctggc tctctacaag tcgataccgc agtcctacaa gagctccacg
     2041 atggcgccgc gagccctttt cgacgccggg cggctggaag aacagaagaa cgcctgggac
     2101 gaggcgcaga aagaatacgc ggcgatcgac acggattacc cgcagagcga ctggaaccag
     2161 ctcgccaagg accggttgat ccagctcaag gtgctgggaa agataaagta gcgcggaagg
     2221 ctcaacagag acaccgcggc gggtgccgca gcggagggga gggcgcatgg cgcagaagaa
     2281 gtggacggcc agattcctgg agacgaagta cttcggactc gtcatcgggc ttgtcgtctt
     2341 cgtgctcctt ttcctcctca cattccagac caccatcgta ggctatgtcg agcagaagct
     2401 cctggacttc aacttccggg tgaagtcgac cgtcggaagc gcgcgggttc aggagggcgt
     2461 gtccgtggtc caggccaacc cccggatttc accggacatc ctgatcgtgg gcatagacga
     2521 caaggctctg gccaggttcg gacgctggcc gttccctcgc ttccgacacg cggacctcat
     2581 caacaacctg gctcggatca agaaccagga cgaccgggag cgggcccttt tcgtcgacgt
     2641 cttctttatc gagccgtcgg aggtcccgga ggacgacgcc ctgctggtct ccagcatcaa
     2701 gtccaacgga agggtcttcc tggagacggt actgaccctg gatgagaacc cgcccggcac
     2761 ggatgaggag ttcttcggac gagaaagcct cctggagtcc acctacggga ccatcacgaa
     2821 cgtccgtgga gactggctca agacgaacac cttcttcggc gtgcagtctc ccctcaagcc
     2881 ctactcacga gccacatatg ggtacggcca cgcgaacttc cgtgaggacc cggaccaggt
     2941 ctatcgcagc caaccgctgg tggtgaagct ttcccagctg gaaaaggaga tcccgctgga
     3001 caagctcacc gtgaacgagc ccatcgatcg ctcccatttc gaacgcctcg cctggatcga
     3061 caagcagaac gagactcacg aggttccgta tccgttgacc gcgggtgtcc tggaaggtct
     3121 caaccgtcag atgacccgca acgcgcccct caaggtagtt gaggccagcg acacgcaggc
     3181 ggcggcttcc tactacgtgg tgcgcaagta cagagacacg ttcctccctt ccatcaccct
     3241 gtccctggcg ctggagtaca tgcataagaa gctctcggac atcgaggtgg tgatgggcca
     3301 gtatattcgg
//
LOCUS       DASRZS010000255         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5534217, whole genome shotgun sequence.
ACCESSION   DASRZS010000255 DASRZS010000000
VERSION     DASRZS010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5534217"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFH83_06330"
     CDS             complement(<1..626)
                     /locus_tag="VFH83_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6486016.1"
                     /translation="MTDRAIVVTGASTGIGRECVRLLAAEGYRVFAGVRTSQDAQALL
                     QEVPRGLTPVFLDVTDAASIRAAFQTVSQELKDGLFGLVNNAGITVAGPLEVLPIDAL
                     RRQIEVNVIGTVAVTQAFLPLLRKAGGRIVLMGSILGRVALPFVGAYSISKFALEAVT
                     DSMGMELAGSGVSVSLMEPGNIATPIWAKSKQGFLDTTNDAPGMALYRA"
     gene            complement(645..1409)
                     /locus_tag="VFH83_06335"
     CDS             complement(645..1409)
                     /locus_tag="VFH83_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET6486017.1"
                     /translation="MAKVRYEEMLPHEIVAARTRCPIAYLPIGTIEWHGEHNPVGLDA
                     VKIHALAMRCAETAGGLVFPALFYGEPRDHYQMEASHDEDGQIRGTMRLPEGSFAPGF
                     MGEGQTEADVRYVELLIHVLKQLRSLGFKVMIIMAGHYPLLAHANAAAALYGLQAAGT
                     TAWACTGYELVRGEIPDAGDHAAAWETSLMMVLRPDLVDLSRLSPDPAVPPIGIRGRD
                     PRTHASVEYGRKGVDACVKAITGKALALLDQVRTTD"
     gene            complement(1552..1977)
                     /locus_tag="VFH83_06340"
     CDS             complement(1552..1977)
                     /locus_tag="VFH83_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007991867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET6486018.1"
                     /translation="MRFMVIVKATEASEAGVMPNTKLLTEMGKFNERLATAGVMLEGA
                     GLQASARGARVRFSGGKTTVMDGPFAETKELIAGYWIWKAASRQEAIDWVRQAPFGEG
                     DVIEIRQIAEADDFGEEFTPELREQEARIGKQIRASGTK"
BASE COUNT          329 a          738 c          634 g          360 t
ORIGIN      
        1 gcccggtaga gcgccatgcc gggagcatcg ttcgtcgtat ccaggaatcc ctgtttcgac
       61 ttggcccaga tcggcgtggc aatattgccg ggctccatga gggacaccga cacgccggat
      121 cccgcgagct ccattcccat cgaatcggtt accgcctcca gggcgaactt ggagatggag
      181 tacgcgccga caaagggcag agcaacgcgg cccaggatcg atcccatgag cacgatcctg
      241 ccgccagcct tgcgaagcag cggaaggaat gcctgcgtta ccgccaccgt gccgatgacg
      301 ttcacctcga tctgacggcg aagcgcgtcg atgggcagga cctccagcgg acccgccacg
      361 gtgatccccg cgttgttcac gagaccgaac aatccgtcct tcagctcctg ggatacggtc
      421 tggaacgcgg cgcggatgct tgccgcgtcc gtcacgtcca gaaagaccgg cgtgaggccc
      481 cgcgggactt cctggagcag cgcctgcgcg tcctgggacg ttcggacccc ggcaaacacc
      541 cggtatccct ccgcggcgag aaggcgaacg cattccctgc caatgccggt ggacgcaccg
      601 gtcacgacga tggcgcgatc cgtcatgcgt ggttccgctc ggcctcagtc ggttgtgcgg
      661 acctggtcca ggagcgcgag cgccttgccg gtgatcgcct tgacgcaggc gtccacgccc
      721 tttcggccgt actcgaccga ggcgtgcgtc cgggggtctc ggccgcggat cccgatgggg
      781 ggaaccgcgg gatccggtga cagacgggac aggtcgacca ggtcgggccg aaggaccatc
      841 atcagggacg tctcccaggc cgcggcatga tcgccggcgt ccgggatctc tccacggacc
      901 agctcgtacc ctgtacaggc ccaggccgtc gttccggcgg cctgcaggcc gtacagcgcg
      961 gccgccgcgt tggcgtgcgc caggagcggg tagtgacccg ccatgatgat catgaccttg
     1021 aacccgagac tgcgaagctg cttcagcacg tggatcagaa gctccacgta gcgaacgtcg
     1081 gcctccgtct ggccttcccc catgaacccg ggcgcgaagc tcccctccgg cagcctcatc
     1141 gtgccacgga tctgaccgtc ttcgtcatgg gacgcttcca tctggtaatg gtcccgcggc
     1201 tccccgtaga aaagcgcggg gaagaccagt ccgcccgccg tctccgcgca ccgcatggca
     1261 agcgcgtgga tcttcaccgc gtccaggccc acagggttgt gctccccgtg ccattcgatc
     1321 gtccctatgg gcagataggc gatggggcac cgcgtccgcg cggcgacgat ttcgtgggga
     1381 agcatctctt cgtagcgaac cttcgccatg ccttcacctc tcaccaagga cctgggtttc
     1441 cgctgagagg tcagtgtatc cgcccctggg caacggcgca atgcgcgccg ccacgatccc
     1501 gcgatcgccg cgacccgcgc gctcgcgccg tctccaggct ctcgatcccg cctacttcgt
     1561 cccgcttgcg cggatctgct tgccgatccg cgcttcctgc tcgcggagct ccggtgtgaa
     1621 ctcctcgccg aagtcgtccg cctccgcgat ctggcggatc tcgatcacgt ctccttctcc
     1681 gaatggcgcc tggcggaccc agtcgatggc ctcctgccgg gaagcagcct tccagatcca
     1741 gtagccggcg atcagctcct tggtctccgc gaacggcccg tccatcaccg tggtcttgcc
     1801 gccggagaac ctcacgcggg cgcctcttgc gctggcctga agacccgcgc cctccagcat
     1861 cacgcccgcc gttgccagcc gctcgttgaa ctttcccatc tcggtgagga gcttcgtgtt
     1921 cggcatcacg ccggcctccg aggcttccgt tgccttgaca atgaccatga accgcatctg
     1981 cgtacctcct gtgcatccgg tagtcgaacg acggggtccg gatcgacacg ccagctcact
     2041 cggcgagctc ctccagccga c
//
LOCUS       DASRZS010000256         3325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5535231, whole genome shotgun sequence.
ACCESSION   DASRZS010000256 DASRZS010000000
VERSION     DASRZS010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5535231"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFH83_06345"
     CDS             complement(<1..473)
                     /locus_tag="VFH83_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6486019.1"
                     /translation="MTGRKGGLTGFLTVSSFLAPSLIGFIVFLFGPMVASIIFSLNDW
                     NILSPMKFVGLGNYTHLLHDKLWWTSLGNTFWYSLLNIPLVIAVSIVFALVVTRRGRL
                     SSTIFRTIYFLPVVVSEVAVAIMWKWILSTNYGFLNAGLESLGLPKVGWLTDGRVA"
     gene            complement(545..1828)
                     /locus_tag="VFH83_06350"
     CDS             complement(545..1828)
                     /locus_tag="VFH83_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET6486020.1"
                     /translation="MRKSIGLWGVCLVVLAALLIVAPSATAGGSSEKTTGNVTLTWAG
                     WDPISKYQPLIDQYAKNKPSVTIQYTSYPDYKTKMLTSAASGTLPDILGIGTGYVLDF
                     GAAGILKEVGPALKDDPSYDFNDIWPSALPFITYNGKIYAAPSDGGTYAMVYNKKMFD
                     AAGVPYPTDKWTWDDFLAAAKKLTITQGGKTRYATFVLANGFDGMYGYVRQNGGDIFN
                     ADHTKSMLDQPAAWQAMQWVKDLDTKYHVAPPIESTSNIPLPIASMINSGTIAMGRVG
                     MWETHVLKDSDILDWAATPMPAGVAGHSEILYLNGLGVAASSKVGNDAVDFLKFATSK
                     TGEEILLRHTSDPQIAVRKSLKEVAISSFEAKRNAGAWIDAMAYSQYYPLIPKLSEVI
                     GVADREMSKALIEGQPAQQVFATIAADQNAVLSGQ"
     gene            complement(1900..>3325)
                     /locus_tag="VFH83_06355"
     CDS             complement(1900..>3325)
                     /locus_tag="VFH83_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486021.1"
                     /translation="GTPERHFLIGVFASPHFLQFGRDYSGAPDQWVYAYSSAGDDGIA
                     TWSAGDQTYLGRVPRDKILEQSSWEFFCGSGPRDVRWSRNVAEARPVFSYPKKTGENE
                     VVFHPGLGRYLLLNWAYIDCTNHTIGSLHSELSVFEAPKPWGPWETVHVLRDWGHNCD
                     YQPRLPAKWMDPMGPGAWLVSAGNFRQIGGRIHYGFGTVPVRFGTGDFGAAAGPAAGG
                     PATAGGSAPSELGDFSALPLSDTEIELSWLPVQGVGFYRIERDGVKLKDIAPCYPARF
                     IDTGLPPAGRHEYRVHAFSPAGDAMESAGPSAAQTLPATEGRSFGVNLAGPGLFDGET
                     LWLGQDSQTRFSVVTAGAFQDSRIRTLPLRPPPGALEGILRGFRGEVSGVKVVFRGLS
                     GKKLKVWVYVVDPRLCGTKVQFGVLLQGVLVETYRTSLPGSDNWGAMGPYDVQADSSG
                     EVILEGRNFTGLGGIAAARIQVLG"
BASE COUNT          610 a         1085 c         1040 g          590 t
ORIGIN      
        1 gcgacccggc cgtccgtcag ccagccgacc ttgggaagac ccagggactc cagaccggcg
       61 ttcaggaacc cgtagttcgt gctgaggatc cacttccaca tgatggccac ggccacttcg
      121 gataccacca cgggcaggaa gtagatggtg cggaagatgg tggagctgag ccggccgcga
      181 cgggtcacca ccagggcgaa gacgatggaa acggcgatga ccagcgggat gttgagcagg
      241 gagtaccaga aggtgttgcc gagggaagtc caccacagct tgtcgtgcag caggtgggtg
      301 tagtttccca gcccgacgaa cttcatcgga gagaggatgt tccagtcgtt caggctgaag
      361 atgatcgagg cgaccatggg cccgaacagg aagacgatga agccgatcag gctgggagcc
      421 agaaaggatg agacggtgag gaaaccggtg agcccgccct tcctgccggt catgagcgaa
      481 tctggcctcc cctggcgcgt cggcgcaccg ccccgcggcg ggcgccgaca tgccgctgaa
      541 ccatctactg tcccgagagc accgcattct ggtccgcggc gatcgtggcg aaaacctgct
      601 gggccggctg accctcgatc agggccttgc tcatctcccg gtccgcgacc ccgatcacct
      661 cgctcagctt cgggataagg ggataatact ggctgtacgc catggcgtcg atccaggcgc
      721 ctgcgttgcg cttggcctcg aagctggaga tcgcgacttc tttcagggac ttgcgaacgg
      781 cgatctgagg atccgaggtg tgccgcagga gaatctcctc cccggtcttg gatgtggcga
      841 acttgaggaa atccacggcg tcgttgccga ccttgctgga tgcggcgact cccaggccgt
      901 tgagatagag gatttccgag tgccccgcga cacccgctgg catgggcgtg gcggcccaat
      961 ccagaatatc gctgtccttc aggacgtggg tctcccacat cccaacccgg cccatggcga
     1021 tcgtcccgga attgatcatg ctggcgatgg gaagggggat gttgctggtg gactcgatcg
     1081 gcggcgccac gtggtacttc gtgtcgaggt ccttgaccca ttgcatggcc tgccaggcgg
     1141 cgggctggtc cagcatagac ttcgtgtggt cggcgttgaa gatgtctccg ccgttctggc
     1201 ggacatagcc gtacattcca tcgaagccgt ttgccaggac aaaggttgca taccgggtct
     1261 tgccgccctg ggtgatcgtc agcttcttgg ccgcggccag gaaatcgtcc caggtccact
     1321 tgtccgtggg gtacggaact cccgcggcgt cgaacatctt cttgttgtac accatcgcat
     1381 aggtgccgcc gtcactgggc gccgcgtaga tcttcccgtt gtaagtgatg aacggcagcg
     1441 cgctgggcca gatgtcgttg aagtcatagc tggggtcgtc cttcaacgcg ggtccaacct
     1501 ctttcaggat accagccgcg ccgaagtcca ggacgtaccc ggtcccgatg cccaggatgt
     1561 ccggcagcgt ccccgatgcc gcgctcgtga gcatcttcgt cttgtagtcg ggatacgacg
     1621 tgtactggat ggtcaccgat ggcttgttct tggcatactg atcgatcaaa ggctgatact
     1681 tgctgatggg gtcccatccc gcccacgtca gcgttacgtt ccccgtggtc ttctccgagg
     1741 aaccgccggc ggtcgcgctc ggcgcgacga tcagcagcgc cgcgagcacc accagacaca
     1801 ccccccagag cccaatgctt ttcctcatat tcgaactcct tcgtgaaccg tttcgcacag
     1861 tcgcattctc gagccgatca ccctggcgaa tcccgccagt cagcccagga cctggatcct
     1921 ggccgcggcg attccgccaa gccccgtgaa gtttctacct tccagtatca cctccccgga
     1981 gctgtcggcc tggacatcgt acggccccat cgctccccag ttgtcgctcc ccggcaggga
     2041 ggtccggtag gtttccacca ggacgccctg gagcaacacg ccaaactgga ccttcgtgcc
     2101 gcagagcctg gggtccacaa cgtagaccca gaccttgagc ttcttccccg acaaccctcg
     2161 gaacaccacc ttcacgccgg acacctctcc tcggaatccc cggaggattc cttccagcgc
     2221 ccccgggggc ggacggagag gcagcgtccg aatccgcgag tcctggaagg cgcccgcggt
     2281 cacaacggag aacctggtct gcgagtcctg cccgagccag agggtctccc catcgaacag
     2341 ccccggtccc gcgaggttga cgccgaagga gcgaccctcc gtcgccggca gggtctgcgc
     2401 cgcgctcggc ccagcggact ccatggcgtc gccggccggt gagaaagcat ggacgcgata
     2461 ctcgtggcga ccggcaggag gcaggcccgt gtcgatgaac cgggccggat agcagggcgc
     2521 gatgtctttg agctttacgc catcacgctc gatgcgatag aaccccacgc cctggactgg
     2581 cagccaggaa agctcgatct ccgtgtcact gaggggcaag gcactgaagt cgcccagctc
     2641 ggacggggcg gagccgccag ccgttgccgg cccgccagcc gccggtccgg cggccgcccc
     2701 gaagtcgccc gtaccgaagc gaacggggac cgtcccaaac ccgtagtgga tcctcccgcc
     2761 gatctggcgg aagttgccgg cggataccag ccaggcgccc gggcccatgg ggtccatcca
     2821 cttcgccggc aaccgaggct gatagtcgca gttgtgtccc cagtcccgca gcacgtgaac
     2881 ggtctcccac gggccccacg gtttgggagc ctcgaatacg gaaagctcgc tgtgcaagga
     2941 gccgatcgtg tggttcgtgc agtcgatgta ggcccaattc aggaggaggt accttcctag
     3001 cccgggatgg aagacgacct cgttctcccc ggtcttcttg gggtaggaga aaaccgggcg
     3061 tgcttccgcc acattccgag accaccgaac atcccgcggt cccgagccgc agaagaactc
     3121 ccatgacgac tgttccagga tcttgtccct ggggaccctc cccaggtagg tctggtcccc
     3181 ggcgctccag gtagcaatcc catcatcacc cgcggaagag tacgcgtaca cccactggtc
     3241 cggcgcgccc gagtagtccc tgccgaactg caggaaatgg ggagaggcaa acacgccgat
     3301 gaggaagtgt cgctccgggg ttcct
//
LOCUS       DASRZS010000257        12261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_916933, whole genome shotgun sequence.
ACCESSION   DASRZS010000257 DASRZS010000000
VERSION     DASRZS010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 12261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12261
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916933"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2965)
                     /locus_tag="VFH83_06360"
     CDS             complement(<1..2965)
                     /locus_tag="VFH83_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET6486022.1"
                     /translation="MPFLLIVDLTSFGLSLMVSTSLALIVAGGDLRSPLNRSFLLFAL
                     LETAWAVLSLLLRLALWLGQGAPEHLLWGGTLSFALMGPLLLLFAARQARLRSRWATY
                     VAIAAVALIAAAAYPLFQGHLISGPTLSAAGLIRYELAPLGYLGALIPGSCFVLSVVV
                     FVTSRREGSQRFMAVSAVLLLLGLAVGALERLGLPLMSVATLTSVSVLGWVIVRRQLF
                     NPLRELTGDLRERAHRQELVAKVSQSTTATLEMNELLLQAARLVQSSFQYFRVGIFLI
                     EGDQLVLMASTYPAAQAYSGKFRLTVGKEGICGWVAASGQSLLVGDVSKEPRYVKLVE
                     SVTARSELAVPIRRGKKVIGVLDVQSALPNAFTASDLFTLQTIADQLSSAIENARLYE
                     ETRRRAERLSLVNRISSAAGSVLDLADLLETVYREMTHIFGADAFYLALYDPVTNSLD
                     YRLQVDEGLRIPQEREPVGVGLTSRVIVERRPLLVNDPASSTEHPPPETWGTGKVPIS
                     WIGVPMLSRDRLVGVISVQTYREHHYDAEDLQLAATIADQVAVAVENARLYEEARQEL
                     AVRQRAEKVLRESEEKFRNLAEQSPNMIFIWSSGKVVYANRQCSVMMGYTRAEFYDPA
                     FDFMVLTASGFESIVMDNLARHRRGEEVPPYEYGLVTRQGRRIDAILTSKLIRYAGAP
                     AILGTITDITTRKHAELLLHSLNSAALAMERALHPAEIFPLALRELASQGLQCAVRPG
                     AGAAGPGGRSGPGTGPDRAPGRGLAAGSAPPERAGAACRPLAPIGPLDHRQRPRHPGA
                     AGRERRGVRPPGGGRGARLRGPADPDRLRSPGGGCLAQDPAHAGSGAEPPAAPPDPGA
                     VPPCPEDGGNRPVRRRHRARVQQSPHGDFRALQPPGGCPDGQRIRAGAARPDPCGHQA
                     GVHPYRSPLDLQSKADEPSGGAGSEQGGIQRGRSARAGHRRGHRAHRPAHGEPGLPAR
                     GRRADGADHHQPGRKR"
     gene            complement(3035..3517)
                     /locus_tag="VFH83_06365"
     CDS             complement(3035..3517)
                     /locus_tag="VFH83_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET6486023.1"
                     /translation="MSLTRHPMRRAEREITRPEDLAEVFREAEVLFLALQDDPAPYVI
                     PLSFGWEPGRLYVHTAREGRKLDLVRRNPAVGFSACAGQQVVAAETACDLSVQGRSVV
                     GAGRARILEQEAERRHAMDVIVRHYAPGALETAYRPASLARADLIEISIESLRGKRMG
                     "
     gene            complement(3650..4702)
                     /locus_tag="VFH83_06370"
     CDS             complement(3650..4702)
                     /locus_tag="VFH83_06370"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6486024.1"
                     /translation="MIRLGIIGTGGMANAHAKSYQPMKGLSLTACCDIDEKRAREFSE
                     RWSIPRWYTDYREMLGAETLDAVDVVTVDAAHAPVTLAAIGKGLSVMCEKPLATSLAD
                     ARKMRDAAKKRGVLTMVNFSYRNSSGAQAASQLIRDGGIGRVIHVESSYLQSWLAQNA
                     WGDWRTQTGMTWRLSTRHGSAGVLGDIGCHIYDLTALLCGDIAEITCLLSTFDKGIKG
                     NRIGEYVLDANDSFVSTIRLAGGGLGTVHSSRWATGHLNSLRVRVYGDRGAIEVDLDR
                     SYTSYRICRGANLKKAQWNEVSCKRTPTQYERFIHVLKTGKPDVNDFANGAKVQAYLD
                     ASLRSDETRQPVRVKL"
     gene            complement(4823..5191)
                     /locus_tag="VFH83_06375"
     CDS             complement(4823..5191)
                     /locus_tag="VFH83_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HET6486025.1"
                     /translation="MYCASCGKELPAQAAFCPACGAAATGATNAAHGGAGTQGNLRPY
                     PSRSGVDVSQKSRLAALLLCLFLGWLGVHRFYVGKVGTGVLMIFTFGGLGIWTLIDLI
                     MIIVGGFKDADGKFVLDWQV"
     gene            complement(5253..6410)
                     /locus_tag="VFH83_06380"
     CDS             complement(5253..6410)
                     /locus_tag="VFH83_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6486026.1"
                     /translation="MKRDTVKLFRTIENLYTIKDLDSLLENVLTEARKFVNADAGTIY
                     LANQGRLFFSFVQNDTLFRGETKDRYITSDVGMPIDRSSLAGYVAASGETILIDDVYH
                     IHSNVSYSFNPDYDQKTSYRTQSILGVPLRTRDNEVVGVLQLINAKNERGEVVAFSMQ
                     DRLFIAQFALNAADAIEKARLSREMVLRMVELAELRDPYETSQHAKRVAAYAVELYEQ
                     WALRRGMAAREIRRVRDVLRTAAILHDVGKVAVSDVILKKREALTYDEKLAMRMHTVL
                     GARLFKRTSSLWDYMSAEVALNHHENWDGSGYPGPGKQGAEIPLSARVVSIADVYDAL
                     ISQRAYKMGWRPEHALRYIRYQAGRKFDPELVGLFLRMGDVLNAIARKYSY"
     gene            complement(6455..7144)
                     /locus_tag="VFH83_06385"
     CDS             complement(6455..7144)
                     /locus_tag="VFH83_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HET6486027.1"
                     /translation="MALRYRCILIDHDDTAVDSTSAVHYPAHLEALRVLRPNRIPPTV
                     EQWLLWNFDPGIMEYLVDELAMTKEELDVEFRIWRSFTSDRTPPFFPGFLDLLADYRA
                     AGGLVVVVSYSERDIIERHYRAASDPPFQPDLIFGWDPDESRRKPNPWPVQEALRLLD
                     CAAEESLIVDDLKPGILLSQASGVAFAAAGWGHRVPQIESYMRANASVYCDSVEALRR
                     HLFPVPDHARP"
     gene            7262..8224
                     /locus_tag="VFH83_06390"
     CDS             7262..8224
                     /locus_tag="VFH83_06390"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HET6486028.1"
                     /translation="MRREKVGRFTAAAARCALCLLFAGLLVSCSINRLAVRTVAGFLS
                     SSGTSTVFTGDDDPELVRDALPFALKTYESLLAQDPGNAPLALTTGSAFASYAYAFVQ
                     IPSDELPADQVDRQQVGHERAKRLFLRARRYVLQGLDVRRPGFSAALEGQSADAALRL
                     VHRDDIDYLYWTAATWLGAFSADPYDFSLIVAIPRAVTILQQVLSWDPGYGEGGAQQI
                     LISFYGTAPANLGGSEEKARESYGRAVQYSKGHRAGPYIALATAVSVKRQDAAEFRDL
                     LARALAVDPNAAPADRLQNTIDQRRARWLLDNMDRFFLDQGDNG"
     gene            8221..9228
                     /gene="dctP"
                     /locus_tag="VFH83_06395"
     CDS             8221..9228
                     /gene="dctP"
                     /locus_tag="VFH83_06395"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET6486029.1"
                     /translation="MRRCVTLVAALTLGLWAAGTGAALTIKLGSLAPAGSPWELGLKR
                     MAAEWDRISQGSVTVRVYTGGIAGDEPDMVRKMRIGTLNAALVTVSGLQGIFNGVKTL
                     SYPLLMKNDEELQSVLQHMKPYFTQKLVSRGFVPIVWSVGGWVYFFSRYPVVSPDDLR
                     KQKLWVWSGDPDEIQAYQNAGFQTVTVAATDLMTSLQSGMVDALVTSPLLAAANQWFA
                     VASNMSTMRLGPLWGAAIVSTRTWAEVPADLQPRLIDAAQRIFDALAPDLLKQDQDAI
                     EVMKRYGLKVNPITPPASAQWDTLLNRTFSGLVGKLYDQESFDTVKAYLDEYHAAHPQ
                     R"
     gene            9197..11215
                     /locus_tag="VFH83_06400"
     CDS             9197..11215
                     /locus_tag="VFH83_06400"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET6486030.1"
                     /translation="MSTTPRIPSAERRGFTLAALARGSVSTVVVLLLALLAALPLVQV
                     VARELSRAGIRGSDAYLQHLVLWVAFAGSIVTAREGRHLSLSVASDRLPRRVQRIARA
                     AASAVSGFVGTVLTFASLGFAAVGFSPSARAGAIPVWILVMIMPLSYAAVTVRLVARA
                     AAPLEAEGGTTAAGGGRSPAASGGPGAGSIAALMVALVGVLLGIAWSLSAISDSLQIA
                     ATAPGALGRAAQGLGNAASAGSEVLAPIVAAAHLPLIALILMGTLLGAPIFVLLGGLA
                     VLLFLHGGGPLEVIPNQAYTMLSRPVIPALPLFTLAGYIISESKAGERLVRLFRGLLG
                     WLPGGLAIMSVVICAFLTTFTGASGVTILVVGSLLLFVLTEGRLSRSFSSGLLTAAGS
                     VGLLFPPSLPVILYGVVAQVSIEQLFVGGILPGVFLVLTLAALGIRRSIRDKVPRVPF
                     DPAEALRGLGASVFEVLLPVLILVLFLRGIMTLVETAAFSVAYVLVVEVVIHRDIPVR
                     RLPEVFLKGAVVVGGVLTILAVANGLSYYTVDAQIPSQLAAWLRSSVASKYLFLLILN
                     LSLLVVGAFMDIFSAITVVAPLVIPMAAAYGINPVHLGIIFLANLELGYLAPPVGLNL
                     QFASYRFAQPMATVVRNVLPFLVVQIVAVLVITYVPGLTTVLLPLTRR"
     gene            complement(11224..>12261)
                     /locus_tag="VFH83_06405"
     CDS             complement(11224..>12261)
                     /locus_tag="VFH83_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6486031.1"
                     /translation="PTEAELEQTYGVSRITVSRAILELQNQDLVYRVKGSGTFISNQA
                     VKAGSGRASAGMSIISAIFPQGEQNGAHDMLIGIESECAKDGFYVTIHNSRNKSYVER
                     ATIGNLLAQGCGGLIVFPCYATHQNAGIYSELLIQCFPTVFVDRHVDYLDIPLVACDN
                     EGAMRDLVGRLIERGHTRIGFFCNSIETVSSEHERFQGYCKALVAARLPVQPSLIFRR
                     YKGLDKSAEIFDVDEVTHKHYADAALSHFLEAKERPTCVVAVNDILAIALMKAALAKG
                     MKVPGDLAITGFDDLYLSEHVEVPLTTVRQPFERIGVAAARLLLDRLKRAPQPQADVR
                     LPAEIIFRQSA"
BASE COUNT         1886 a         4065 c         4186 g         2124 t
ORIGIN      
        1 ggcgtttacg gccaggttgg tgatgatctg ctccatctgc ccggcgtccg cgcgcaggta
       61 gaccgggctc tccgtgagct ggacggtgag ctcgatgtcc tcgccgatga ccggcgcgag
      121 cagaccggcc gcgctggata ccaccttgtt cagatccagc tccaccggac ggttcatctg
      181 ctttcgactg gagatcaaga ggcgaccggt aagggtggac gcccgcttga tggccgcacg
      241 gatctggccg agccgcgcct gcgcggattc gttgtccgtc agggcatccg ccaggaggct
      301 ggagtgcccg gaaatcaccg tgaggagatt gttgaactcg tgcgcgatgc cgccggcgaa
      361 ccggccgatt gcctccatct tctgggcatg gaggaactgc tcctgggtct ggcggagctg
      421 ctggaggctc cgctccactt cctgcatgag ccgggtcttg cgccaggcag ccgccgcctg
      481 gtgagcgaag gcgatcagga tctgctggtc ctcgaagtcg agctccccga ccaccaccag
      541 gaggccgaac acctcttcgc tcacgaccag cggcgccagg atggcgcggg cgctgtcggt
      601 ggtcgagcgg cccgatcgga gcgagaggcc ggcatgccgc tccagcacgc tcaggagggg
      661 cggatccagc agcgagaccg cggcctggcg cgcgatcagg acctgtgcca gggccggagc
      721 gtcctccagg gccagcggct cccgctcctg gtcggacggc gcactgcagc ccctgggatg
      781 cgagctcgcg aagggcgagg gggaagatct ccgcggggtg gagcgcccgc tccatggcca
      841 gggccgcgga gttgagagag tgcaggagga gctcggcatg cttgcgcgtc gtgatgtccg
      901 tgatggtgcc caggatggcc ggagccccgg cgtagcggat gagcttcgag gtgaggatgg
      961 cgtcgatgcg ccgtccctgc cgcgtgacca acccgtattc gtacggaggg acctcctcac
     1021 cgcgtcgatg gcgcgccagg ttgtccatca cgatcgactc gaagcccgac gcggtgagga
     1081 ccatgaagtc gaaggcgggg tcgtagaact cggcccgggt gtaccccatc attacggagc
     1141 actgccggtt ggcgtagacg acctttcccg atgaccagat gaagatcatg ttgggggact
     1201 gctcggcgag gtttcggaac ttctcttcgg actcgcgaag gaccttctcg gcgcgctgcc
     1261 ggaccgcgag ctcctgtcgg gcctcctcgt acagccgggc gttctccacg gccacggcca
     1321 cctggtccgc gatggtggcg gcaagctgca ggtcctccgc gtcatagtgg tgctcgcggt
     1381 aggtctgaac gctgatcacc cccaccaacc ggtcccgcga gagcatgggg acgccgatcc
     1441 aggagatggg caccttgccg gtgccccagg tctccggcgg gggatgctcc gtcgagctgg
     1501 ccggatcgtt caccaggagg ggcctgcgct cgacgatcac gcgggaggtc agccccacgc
     1561 cgaccggttc ccgctcctgc gggatccgca gtccctcgtc cacctggagg cggtagtcca
     1621 ggctgttggt gacggggtcg tagagggcca ggtagaaggc atccgcgccg aagatgtgcg
     1681 tcatctcgcg gtagaccgtc tccaggaggt ccgcgaggtc gagcaccgag ccggccgcgg
     1741 aggagatccg gttcaccagg gagagcctct cggcgcgccg gcgggtctcc tcgtagaggc
     1801 gcgcgttctc gatggcgctg gagagctggt ccgctatggt ctgcagcgtg aagagatcgc
     1861 ttgccgtgaa cgcgttcggc agggcgctct gcacgtccag cacgccgatc accttcttgc
     1921 cgcggcggat ggggaccgcc agctcggacc gggcggttac cgactccacc agcttcacgt
     1981 agcgcggctc cttgcttacg tcgccgacca gcagggactg cccgctcgcc gcgacccacc
     2041 cacagatccc ctccttgccc acggtgagcc ggaacttccc ggagtacgcc tgggccgccg
     2101 ggtaggtgga ggccatcagc acgagctggt ctccctcgat caggaagatc cccacgcgga
     2161 aatactggaa ggacgactgg acgagacggg cggcctggag caggagctcg ttcatctcaa
     2221 gggtggcggt cgtgctctgg ctcaccttcg ccacgagctc ctggcgatgc gcccgttcgc
     2281 gaaggtcccc ggtgagctcc cgcagcgggt tgaagagctg gcggcgcacg atgacccatc
     2341 ccagaacgga gacgctggtc agcgtggcca cggacatgag cgggaggccc agccgctcca
     2401 gggcgcccac cgcgaggccc aggaggagga gcacggcgct cacggccatg aaccgctggg
     2461 agccttcccg ccgggaggtg acgaagacga ccacggagag cacgaagcag gagccgggaa
     2521 tgagcgctcc gagatagccc aacggggcga gctcgtagcg gatgagtccc gccgccgata
     2581 gggtggggcc cgagatcagg tgtccctgga agagcggata agcggcggcg gcgatcaagg
     2641 ccacggccgc gatggcgacg taggtggccc atcgggaccg cagtcgggcc tggcgcgccg
     2701 cgaacaggag gagcagcggc cccatcaggg cgaaactgag cgtccctccc cacaagagat
     2761 gctccggggc gccctggccc agccacaggg cgagccgcag gagaagggac agcacggccc
     2821 acgcggtctc caggagggcg aagagcagga aggaacggtt gagcgggctg cgcagatcgc
     2881 caccggccac gatcaaggcc agggacgtgg ataccatgag ggagaggccg aagctcgtca
     2941 ggtcgacgat caggaggaat ggcatggtcg gactgtcacc tcctaccgca ttggccgacc
     3001 gatcgggccc agggtcccat ccgtcggctc ggcctcaccc catccgcttt ccgcggaggg
     3061 actcgatgga gatctcgatc aggtctgctc gggccagcga ggcgggccgg taagccgtct
     3121 ccagcgcgcc tggggcgtag tgacgcacga tgacgtccat ggcgtggcgg cgctccgcct
     3181 cttgctccag gatccgggct cggcccgcgc cgacaacgct ccgcccctgg accgagagat
     3241 cacacgcggt ctccgcggcc acgacctgct gcccagcaca ggcgctgaag cccacggcag
     3301 gattccgccg cacgagatcc agcttccgtc cttctcgcgc cgtatgcacg tagagcctcc
     3361 cgggctccca accaaagctc agcgggatca cgtatggcgc gggatcgtcc tgcagcgcca
     3421 gaaagagcac ctccgcctcc cgaaagacct ccgcgaggtc ctccggccgg gtgatctcac
     3481 gctcggcgcg gcgcatgggg tgacgggtca ggctcatccg gtcgcgttct ccctcaacgc
     3541 cgccgcacgc aagccggaag cgtttcttcc ataatcatag cacacggagt gatacgccgg
     3601 agaaggcgag gtggtttcct ccccctcttg cccggttgca gccgatggtc tacagcttta
     3661 cccgcaccgg ttgccgggtc tcgtccgagc ggagactagc atccagatac gcctggacct
     3721 tggcgccgtt ggcgaagtcg ttcacgtcgg gcttgccggt cttgagcacg tggatgaacc
     3781 gctcgtactg ggtcggggtc cgcttgcagc tcacctcgtt ccactgggcc tttttcagat
     3841 tggcgccgcg gcagatccgg tagctggtat agctgcggtc caggtccacc tcgatggccc
     3901 cccggtcccc atacacgcgg acccgaaggc tgttcaggtg ccccgtggcc catcggctgg
     3961 agtgcaccgt ccccagaccc ccgccagcca ggcggatcgt ggagacgaag ctgtcattgg
     4021 cgtcgaggac atattccccg atccggttgc ccttgatgcc tttgtcgaag gttgaaagga
     4081 gacaggtgat ctcggcgata tccccgcaca gcagggcggt gagatcgtag atgtggcagc
     4141 cgatgtctcc gaggaccccg gcgctcccat ggcgggtcga aagccgccag gtcattccgg
     4201 tctgcgtccg ccagtccccc caggcgttct gggcaagcca gctctggagg tagctggact
     4261 ccacgtggat cactcggccg atgccgccgt cgcggatgag ctgggaagcc gcctgggccc
     4321 cgctggagtt gcggtaggag aagttgacca tggtgagcac accacgcttc ttggcggcgt
     4381 cccgcatctt gcgggcgtcg gcgagggagg tggccagcgg cttttcgcac atcacggaca
     4441 gtcccttgcc gatggcagcc agcgtgaccg gggcgtgggc ggcatcgacc gtcaccacat
     4501 ccacggcgtc cagtgtctcg gcgcccagca tctcgcggta gtccgtatac cagcgcggaa
     4561 tgctccatcg ctcggagaac tcgcgggcgc gcttctcgtc gatgtcgcag caggccgtca
     4621 gggacaggcc cttcatgggc tggtagctct tggcgtgggc gttcgccatc ccgccggttc
     4681 cgatgatccc caggcgtatc atgcggccac tgtattccga cggaacggcg cggtcaatcc
     4741 gatggacccg gttcagctca tgattccgcg atccggctcg cggaatgcta cgcggccctc
     4801 ccgtcagcgg gagcccgacg ggtcagacct gccagtcgag cacgaacttc ccgtccgcgt
     4861 ccttgaagcc gccgacgatg atcatgatca ggtcgatgag cgtccagatt cccagcccgc
     4921 cgaaggtgaa gatcatcagc accccggtgc cgaccttgcc tacgtagaac cggtgcaccc
     4981 cgagccatcc caggaagagg cacaggagca gggccgcaag acggctcttc tggctgacat
     5041 cgactccgct tcgggacggg tacggacgaa ggttcccttg cgtgccggcc ccgccatggg
     5101 ccgcgttcgt ggcgcccgtg gcggcagcgc cacaggccgg acagaacgcg gcctgagccg
     5161 gcagctcttt cccgcagctc gcgcagtaca tacggattgc ctccttccgc aatggaacac
     5221 cacaaggctt ccccggtgtc cccgggtgac cttcagtagg agtacttgcg cgcgatggcg
     5281 ttgagcacgt cacccatgcg gaggaacaga cccaccagct ccgggtcgaa cttccgtccg
     5341 gcctggtaac ggatgtagcg caacgcgtgc tcggggcgcc accccatctt gtaagcccgc
     5401 tgggagatca gcgcgtcgta gacgtcggcg atggagacca cgcgcgccga cagggggatc
     5461 tccgcgccct gtttgcccgg gccggggtag ccgctgccgt cccaattctc gtggtggttg
     5521 agcgccacct ccgcggacat gtagtcccag agcgagctgg tccgcttgaa gagccgggcc
     5581 cccaggaccg tgtgcatccg catggccagc ttctcgtcgt aggtgagggc ctctcgcttc
     5641 ttgaggatga cgtcgctcac cgccaccttc cccacgtcgt ggaggatggc cgcggtccga
     5701 agcacgtcac gcacccgacg gatctcgcgc gccgccatcc cgcggcggag ggcccactgc
     5761 tcgtacagct ccaccgcgta cgcggccacc cgcttggcgt gctggctggt ctcgtagggg
     5821 tcccgcagct ccgccagctc caccatccgc agcaccatct cgcgggaaag acgcgccttc
     5881 tcgatggcgt ccgccgcgtt cagggcgaac tgggcgatga agagccggtc ctgcatggag
     5941 aaggcgacga cctcgcccct ctcgttcttg gcgttgatga gctgaaggac ccccaccacc
     6001 tcgttgtcgc gcgtacgaag gggcaccccc aggatggact gcgtgcggta gctggtcttc
     6061 tggtcgtagt ccgggttgaa ggagtagctg acgttgctgt ggatgtggta gacgtcgtcg
     6121 atgaggatgg tctctccgct ggcggccacg tagccggcca gcgagcttcg atcgatgggc
     6181 atccccacgt cgctggtgat gtagcggtcc ttggtctctc ctcggaacag ggtatcgttc
     6241 tggacgaagc tgaagaacag ccggccctgg ttggccaggt agatggtccc cgcgtcggcg
     6301 tttacgaact tgcgcgcctc ggtgagcacg ttctcgagca gcgaatccag gtccttgatg
     6361 gtgtacaggt tttcgatggt tcggaataac tttacggtat cgcgcttcat cgatgtgggc
     6421 cgcttctata ctataacggt gggacccgtg attctcaagg tcgagcgtgg tccgggaccg
     6481 ggaacaggtg ccttctcagc gcctcgacgc tgtcgcagta cacggaagcg ttggcccgca
     6541 tgtagctttc gatctggggc acccggtggc cccatccggc ggcggcgaac gccacgccgc
     6601 tggcctggct gaggaggatc cccggcttca gatcgtcgac gatcagcgac tcctccgcgg
     6661 cgcagtccag gagccgcagc gcctcctgga ccggccaggg gttgggcttg cgccggctct
     6721 cgtccgggtc ccagccgaaa atgaggtccg gctggaaggg cggatcgctt gcggcgcgat
     6781 agtggcgctc gatgatgtcc cgctcggaat aggagaccac gaccaccaac ccgcccgccg
     6841 cccgatagtc cgcaagcaga tcgaggaatc ccgggaagaa cggtggggtc cggtccgagg
     6901 tgaagctccg ccagatccgg aactccacgt cgagctcctc cttggtcatg gccagctcgt
     6961 ccaccaggta ttccatgatg cccggatcga agttccacag gagccactgc tccacggttg
     7021 gcgggatccg gttgggtcgc aggacgcgga gagcctcgag gtgggcagga tagtgcaccg
     7081 cggaggtgct gtccacggcc gtatcgtcgt gatcgatgag gatgcagcga tagcgcaggg
     7141 ccatggtgtt gcacgactgt agggctcatc ccgcggcacg tcaatccggc tcgagatgag
     7201 gtgagcggcg gaagaggagg ccgtattctc tagccgttgg cctttcggct atattatgcc
     7261 cgtgaggagg gaaaaagtgg gccggttcac cgccgccgcg gcgagatgcg cgttgtgtct
     7321 gcttttcgcc ggcctcctcg tctcctgctc gatcaaccgc ctggccgtgc ggaccgtggc
     7381 gggctttctg tcttcgagtg gaacgagcac cgtgttcacc ggcgatgacg atcccgagct
     7441 ggtgcgggat gctctgccgt tcgccctgaa gacgtacgag tcgctcctgg cgcaggatcc
     7501 cggcaacgcg cccctggccc tgaccacggg cagcgccttc gccagctacg cctacgcgtt
     7561 tgtccagatt ccctccgacg agcttcccgc ggaccaggtg gatcgccagc aggtcgggca
     7621 tgagcgggcc aagcggctct ttctgcgggc ccggcggtac gtcctgcagg ggctggacgt
     7681 tcgtcgcccc gggttcagcg ccgccctgga agggcagagc gccgacgccg cgctccgtct
     7741 ggttcatcgg gacgacatcg actatctcta ttggaccgcg gctacctggc tgggggcctt
     7801 cagcgcggac ccctacgact tctccctgat cgtcgcgatc ccaagggccg tgacgatcct
     7861 gcagcaggtg ctctcctggg accccggcta cggggaagga ggggcgcagc agatcctgat
     7921 ctccttctat gggacggcgc ccgcgaacct gggcggcagc gaggagaagg ctcgggagag
     7981 ctacggccgc gccgtgcagt actccaaggg tcacagggcc ggtccgtaca tcgcgcttgc
     8041 caccgccgtc agcgtgaaac ggcaggacgc cgcggagttc cgcgaccttc tggccagggc
     8101 gctggccgtg gatccgaacg cggcgcccgc cgaccggctg cagaacacca tcgaccagcg
     8161 tcgcgcgcgc tggttgctgg acaacatgga tcggttcttc ctggatcaag gggataacgg
     8221 atgagacggt gcgtgacgct ggtcgcggcg ctgaccctcg gcctttgggc ggccgggacg
     8281 ggcgccgccc tcaccatcaa gctgggcagc ctggcgcccg ccggctctcc gtgggagctg
     8341 ggtctgaagc ggatggccgc ggagtgggac cggatttccc agggctccgt gaccgtgcgc
     8401 gtgtataccg gcggcatcgc cggcgacgag ccggacatgg tgcgcaagat gcggatcggg
     8461 accttgaacg ccgcgctggt gacggtgagc gggctgcagg ggattttcaa cggcgtgaag
     8521 accctctcgt acccgctgct catgaagaac gacgaggagc tgcagtccgt cctgcagcac
     8581 atgaagccgt atttcaccca gaagctggtg agccgagggt tcgtccccat cgtgtggtca
     8641 gtgggcggct gggtgtactt cttcagccgg tacccggtcg tgagcccgga tgaccttcgc
     8701 aagcagaagc tctgggtctg gtctggagac ccggacgaga tccaggcgta ccagaacgcg
     8761 ggcttccaga cggtcaccgt ggcggccacg gacctcatga cgtccctgca gagcggcatg
     8821 gtggacgcgc tggtgaccag tccgctcctg gccgcggcca atcagtggtt tgccgtggcc
     8881 agcaacatga gcacgatgcg gctcggcccg ttgtgggggg ccgccatcgt gagcacgcgc
     8941 acgtgggctg aagtgccggc cgacctgcag cccaggctga tcgacgccgc ccagcggatc
     9001 ttcgacgccc tggccccgga cctcctcaag caggaccagg acgccatcga ggtcatgaaa
     9061 cgctacggtc tgaaggtcaa cccgatcacg cccccggctt ccgcgcaatg ggacacgctc
     9121 ctgaaccgga ccttctccgg gctcgtcggg aagctgtacg accaggagtc gttcgacacg
     9181 gtgaaggcct acctcgatga gtaccacgcc gcgcatcccc agcgctgagc ggcgcggctt
     9241 caccctggcc gcgctggcgc gcggctccgt cagcactgtg gtggttctcc tcctcgcgct
     9301 gctcgcggcg ctcccgctcg tccaggtggt ggcgcgcgag ctgtcccggg cgggcatccg
     9361 cggctcggac gcctacctcc agcacctggt gctctgggtc gcgtttgccg ggagcatcgt
     9421 gacggcgcgc gagggccgcc acctcagcct gagcgtggcc tccgatcgtc tgccccgtcg
     9481 cgttcagcgc atcgcgcgcg ccgccgcgtc ggcggtgagc ggctttgtcg gtaccgtgct
     9541 gaccttcgcc tccctcgggt tcgccgcggt cgggttctct ccgagcgcgc gtgccggggc
     9601 catccccgtc tggatcctgg tgatgatcat gccgctcagc tacgcggcgg tgaccgtgcg
     9661 cctggtggcg agagccgcgg cgcctctgga ggcggagggt gggacgaccg cggcgggcgg
     9721 ggggcgaagc ccggccgcga gcggcggccc gggggccggc tcgatcgcgg ccctgatggt
     9781 ggccctggtg ggcgtcctcc tggggatagc gtggtccctg tcggccatct ccgactccct
     9841 tcagatcgcg gccacggccc ccggagctct gggacgcgcg gcccagggac tggggaacgc
     9901 tgcctcagcc ggcagcgagg tcctggctcc gatcgtcgcg gccgcccacc tgcctctgat
     9961 cgcgctcatc ctgatgggca cactcctggg agcgcccatc ttcgtgctcc tgggaggcct
    10021 cgcggtgctg ctcttcctcc acgggggcgg tcccctggag gtgatcccga accaggccta
    10081 caccatgctc tcccgaccgg tgatcccggc gcttccgctc ttcaccctgg ccggctacat
    10141 catttccgag agcaaggcag gggagcggct ggtgaggctg ttccgagggc tgctgggctg
    10201 gctgccgggc gggctggcca tcatgagcgt cgtcatctgc gcctttctca ccaccttcac
    10261 gggggcctcc ggggtgacca tcctggtggt ggggagcctg ctcctgttcg tcctcacgga
    10321 gggccggctc tcacggtcgt tcagctcggg tctgctgacc gcggccggca gcgtcggtct
    10381 gctctttccc ccgagcctcc cggtcatcct ttacggcgtg gtggcgcagg taagcatcga
    10441 acagctgttc gtcggaggga ttctccccgg ggtcttcctg gtgctcaccc tcgcggcgct
    10501 cgggatccga cgcagcatcc gcgacaaggt gccgcgggtg ccgttcgatc ccgcggaagc
    10561 cctgcgcggc ctgggggcgt cggtcttcga ggtcctgctc cccgtcctga tcctggtcct
    10621 cttcctgcgc gggatcatga ccctcgtgga gaccgcggcc ttctcggtgg cctacgttct
    10681 ggtggtggag gtggtcatcc accgcgatat ccccgtccgc cgtctgccgg aggtgttcct
    10741 caagggcgcg gtggtcgtgg gaggagtcct caccatcctc gcggtggcca acgggctctc
    10801 ctactacacg gtggacgccc agatcccgtc gcagctcgcc gcctggctgc ggtcttccgt
    10861 cgcgtcgaag taccttttcc tgctgatcct gaacctgtcc ctgctggtgg tgggcgcgtt
    10921 catggacatc ttctccgcca tcaccgtcgt ggcgcccctg gtcatcccca tggccgcggc
    10981 ctacggcatc aaccccgtcc acctgggcat catctttctg gccaacctgg agctgggcta
    11041 cctggctccg cccgtcgggc tcaacctcca gttcgcatcc tatcggttcg cccagcccat
    11101 ggcgaccgtg gtgcgcaacg tgctcccgtt cctggtggtt cagatcgtgg cggtgctggt
    11161 catcacctac gtgccggggc tgacgacggt cctgctgccg ctgacccgcc gctgagcggt
    11221 ggatcaggcg ctctgccgaa agatgatctc cgcgggcagg cggacgtccg cctgaggctg
    11281 gggggcccgc ttcagccggt ccagcaggag ccgtgcggca gccaccccga tccgttcgaa
    11341 gggctggcgc acggtggtga gcggcacctc cacgtgctcc gagaggtaca ggtcgtcgaa
    11401 cccggtgatc gccaggtctc ccggaacctt catccccttg gccagggcgg ctttcatgag
    11461 ggcgatggcc aggatgtcgt tgaccgcgac cacgcaggtc ggccgctcct tcgcttccag
    11521 gaaatggctc agggcggcgt cggcgtagtg tttgtgggtg acctcgtcca cgtcgaagat
    11581 ctccgcgctc ttgtccaggc ccttgtagcg gcgaaagatc agggacggct ggacggggag
    11641 ccgggccgcg accagggcct tgcagtaccc ctggaaccgc tcgtgctcgg aggaaaccgt
    11701 ctcgatcgaa ttgcagaaga aaccgatgcg ggtgtggccc cgttcgatga ggcggcccac
    11761 gaggtcccgc atggcgccct cgttgtcgca cgcgaccagc gggatgtcca ggtagtcgac
    11821 gtggcgatcg acgaacacgg tggggaagca ctggatcagg agctcgctgt agatgcccgc
    11881 gttctgatgc gtcgcgtagc aggggaagac gatcagcccg ccgcacccct gcgccaggag
    11941 gttgccgatg gtggcgcgct ccacgtagga cttgtttcgc gagttgtgga tcgtgacgta
    12001 gaagccgtcc ttggcgcact cgctctcgat cccgatcagc atgtcgtggg cgccgttctg
    12061 ctccccctgg ggaaagatgg cggagatgat cgacatgccg gcggacgccc gtccgctgcc
    12121 cgccttgacg gcctggttgg aaatgaacgt cccggagccc ttcacccggt agaccaggtc
    12181 ctggttctga agctcgagga tcgcgcggct cacggtgatc cggctgaccc cgtaggtctg
    12241 ctcgagctcg gcctccgtgg g
//
LOCUS       DASRZS010000258         3943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3992708, whole genome shotgun sequence.
ACCESSION   DASRZS010000258 DASRZS010000000
VERSION     DASRZS010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3992708"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1500
                     /locus_tag="VFH83_06410"
     CDS             157..1500
                     /locus_tag="VFH83_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HET6486032.1"
                     /translation="MKPVRFCFVLLFIFLTAAAFSQDRAAAVLTLDGPIDPVSARYLL
                     RGLQRAQRDGAALVVIQVDTPGGLGTSMDQIVQAILASPVPIVVYVSPQGARAASAGV
                     FVAMAASVAAMAPGTHIGAAHPVDASGADIPGAMGEKVLNDAVAQLKSLAQLRGRDPA
                     WPDEAVRQSQSITADQAVSEHVADLQAASLSDLLAALDGRVVPTAQGAVTLRTTGIEV
                     HRSPMTFIDRFLGVLINPDLAYILLVIGIFGLIFEFSFPGMIAPGVAGALALVLAFLS
                     FGNLPTNIGGLVFIVLSVVLFVVDIKAPTHGVLTAGGVVAFVLGSLLLFPPWRTPTLP
                     TLPVVHVSPVVIAVMTFLVVLFFAVVLAQGVRAQERQVSFGEETIVGMVGRAASELGP
                     DGYVQAGGEQWSARSAEGAIRAGELVEVVGREGLKLVVRRGPTGPAKDTGKEAVV"
     gene            1497..2366
                     /locus_tag="VFH83_06415"
     CDS             1497..2366
                     /locus_tag="VFH83_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014289172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET6486033.1"
                     /translation="MSYVLVGVIVVLVIIILSASIKVVREYQRLVVFRLGRSVGARGP
                     GLVVLIPIVDKPVWTDLRELFLEIPRQTCITKDNAPIDIDFLIYWRVIDPSLSVIQVA
                     NFAGASQGIATTTLRAVIGDIPLDDVLAKREDINRVLRTKLDEVTERWGVKVTTVEIR
                     EIVPPKDIQDAMNRQMSAERNRRAMVIESEGKRQSAILVAEGEKQSAILRAEGDRQSA
                     ILRAEGFALALEKIFGTAKTVDYKTIMLQYFDTLKTMGAGASTKFIFPLEFMNLLKPL
                     AGMAEEAVRKTQG"
     gene            2475..3038
                     /locus_tag="VFH83_06420"
     CDS             2475..3038
                     /locus_tag="VFH83_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486034.1"
                     /translation="MAELVRCKSCGYVMPASRVGARCPACGVDRKMMEPWKDPVSEGR
                     RIRLGFDLHPILDHFSVSFAACAFALVLFELVLPDLFRQTVTSTARAFIGALPVAIVG
                     SFLSGLFDGGTRFRRTTTPLLVRKQILAVAFFVLALGTAGLTFFVGPYIQWVRAVDAV
                     LLAGCVVLAALLGLIGKSLNTAIFPGA"
     gene            complement(3056..3862)
                     /locus_tag="VFH83_06425"
     CDS             complement(3056..3862)
                     /locus_tag="VFH83_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET6486035.1"
                     /translation="MTTNDIRNHLLSVASWVDPKSTVDTVKIGDPDLPIARVGVGWMA
                     TIQDLNVAHRDGCQLFITHEPTFNDHFDPMDGMCRRTEPGRRKAEFLRSTGMVVLRCH
                     DAWDAWPRIGIRDSWARGLGLGEPVGENGEAESQRGWHAVYEIPPVTLREFARMVAMR
                     VKPLGQDGVEVLGDPQRIIRRPALGVGCGGPDQDMVQLGADVLIVCYDGASYWKDRER
                     FVELGVAVLMVEHGTSEMWGMENLARYLTETFPQAAFQYYSGHARAWHVA"
BASE COUNT          576 a         1323 c         1316 g          728 t
ORIGIN      
        1 ggtcatgttc ggcttcttcc agcgtttcat cgtgcagggg ttggcagcgg ccgcggtgaa
       61 aggctgagag ccggtcggga cgagccctac gggccccagg cgctgtagcg cccggggccg
      121 gcttggcagt atactcttgg caggaagggt tccgccgtga agcctgtccg tttctgcttc
      181 gttctcctct tcatcttcct gaccgccgcc gcgttctctc aggatcgtgc cgccgctgtt
      241 ctcaccctgg acggccccat cgatccggtt tcggcgcgct acctccttcg cggtctccaa
      301 cgcgcgcaac gggacggggc ggcgctcgtg gtgatccagg tggatactcc gggcgggctg
      361 ggaacctcaa tggaccagat cgtccaggcg atcctggcct ccccggtgcc tatcgtggtc
      421 tacgtctccc cgcagggggc tcgtgccgcc tcggcgggag tcttcgtggc catggccgcc
      481 tcggtcgccg ccatggcgcc gggaacgcac atcggcgcgg cgcaccccgt ggacgccagt
      541 ggcgcggaca ttccgggagc catgggggag aaggtactga acgacgcggt ggcgcagctg
      601 aaatccctgg cccagctccg gggacgggat cctgcctggc cggatgaggc ggtacggcag
      661 agccagtcca tcaccgcgga ccaggccgtg tcggagcacg tggcagacct gcaggcggcc
      721 agcctttcgg acctgctggc ggcgctggac ggccgcgtgg tccccaccgc gcaaggggcg
      781 gtcacgctgc gcaccacggg gatcgaggtg caccgctcgc cgatgacctt catcgaccga
      841 ttcctggggg tcctgatcaa ccccgatctg gcctacatcc tcctggtgat cgggatcttc
      901 gggctcatct tcgagttctc cttcccgggc atgatcgcgc cgggagtggc cggtgccctc
      961 gccctcgtcc ttgccttcct ttccttcggc aaccttccga cgaacatcgg tgggttggtc
     1021 ttcatcgtgc tctcggtcgt gctcttcgtc gtggacatca aggctcccac ccacggcgtg
     1081 ctgacggcgg gcggggtggt tgccttcgtc ctcggatccc tcctgctctt tccgccatgg
     1141 cggaccccca cgcttcccac gcttcccgtg gtgcacgtct cccccgtggt gatcgcggtg
     1201 atgacgttcc tggtggttct cttcttcgcg gtggtgctgg cgcaaggcgt ccgtgcccag
     1261 gagcggcagg tcagcttcgg ggaggagacc atcgtgggga tggtgggccg cgccgccagc
     1321 gagctggggc ccgacggcta cgtgcaggcc gggggcgaac agtggagcgc gcgctccgcg
     1381 gaaggcgcaa tacgcgccgg agagctggtc gaggtggtgg gtcgggaggg tctgaagctg
     1441 gtggtgcgtc ggggcccgac ggggccggcc aaggataccg ggaaggaggc ggtcgtatga
     1501 gctacgttct tgttggcgtc atcgtcgtgc tggtgatcat catcctctcg gcttccatca
     1561 aggtggtgcg ggagtaccag aggctggtgg tcttccgtct cgggcggtcc gtcggggccc
     1621 ggggacccgg cctggtcgtc ctgatcccga tcgtggacaa gccggtatgg accgacctgc
     1681 gggagctgtt cctggagatc cctcgccaga cctgcatcac caaagacaac gcacccatcg
     1741 acatcgactt cctgatctac tggcgcgtca tcgatccgag cctctcggtg atccaggtgg
     1801 caaacttcgc cggcgcgagc caggggatcg ccaccacgac gttgcgcgcc gttattggcg
     1861 acatccccct ggacgacgtc ctggccaagc gcgaagacat caaccgggtc ctccgcacga
     1921 agctcgacga ggtgaccgag cgctggggcg tgaaggtcac tacggtggag atccgcgaga
     1981 tcgtaccgcc caaggacatc caggacgcca tgaaccgcca gatgtccgcc gagcgcaacc
     2041 gccgcgccat ggtcatcgag agcgagggaa agcgtcagtc cgccatcctg gtggcggaag
     2101 gggagaagca gtccgccatc ctgcgggccg agggcgatcg tcagtccgct atcctgcggg
     2161 cggaagggtt tgccctggcg ctggagaaga tcttcggcac cgcgaagacg gttgactaca
     2221 agacgataat gctgcagtac ttcgataccc ttaaaaccat gggcgccggc gcttccacca
     2281 agttcatttt ccccctggag ttcatgaacc tgttgaagcc gctggccgga atggcggaag
     2341 aggcggtccg caagacccag gggtaggcgc gaccgcgccc cttgaggccg cagcaggccg
     2401 gcctacgtac ggccgggccg cgtacggccg ggccgcgtgc ggctgctctt cccgtttcgg
     2461 ggtcaggagg ggatatggcg gagctggtgc gctgcaagtc gtgtggatac gtcatgcccg
     2521 caagccgcgt cggggcgcgg tgtcccgcct gcggtgtgga ccgcaagatg atggagccct
     2581 ggaaggaccc ggtctccgag gggcggcgga tccgactggg gttcgacctc catcccatcc
     2641 tggatcactt cagcgtgtcc ttcgccgcct gcgccttcgc gctcgtgctc ttcgagttgg
     2701 tgctccccga tctgttccgt cagaccgtga cctcgacggc ccgggctttc atcggcgccc
     2761 tgccggtcgc gatcgtgggc tcgttcctgt cggggctctt cgacggaggg acccggtttc
     2821 gccggactac cacgcccctc ctggtccgga agcagattct ggccgttgcc ttctttgtcc
     2881 tggccctggg tacggccggt ctcaccttct tcgtgggacc gtacatccag tgggtccgcg
     2941 ccgttgacgc cgtccttctg gccggatgcg ttgtgttggc agcgctcctg gggttgatcg
     3001 gaaagagcct caacacggcg atcttccccg gggcctgagc gggctgctca gagcgtcagg
     3061 cgacgtgcca cgcccgcgcg tggccgctgt agtattggaa ggccgcctgc gggaaggtct
     3121 ccgtcaagta ccgggccagg ttctccatgc cccacatctc ggacgtcccg tgttccacca
     3181 tcagcacggc cacgccgagc tccacgaagc gctcgcggtc cttccagtag ctcgccccat
     3241 cgtagcacac gatcaggacg tcggccccaa gctgcaccat atcctggtcg ggtcccccgc
     3301 aacccacgcc cagggcgggc cggcggatga tccgttgggg gtctcccagg acctcgaccc
     3361 cgtcctgtcc caggggtttg acgcgcatcg ccaccatccg agcgaactct ctcagggtga
     3421 ccggcgggat ctcgtaaacc gcgtgccagc cgcgctggga ttccgcctca ccattctccc
     3481 ccaccggctc gcccaggccc aatccccggg cccacgaatc gcggatgccg atccgcggcc
     3541 aggcgtccca ggcgtcgtga cagcgcagca cgaccatgcc ggtgctccgg aggaactcgg
     3601 ccttcctccg accgggctcg gtccgacggc acatcccatc catcggatcg aaatggtcgt
     3661 tgaaggtcgg ctcatgggtg atgaagagct ggcagccgtc ccggtgcgcc acgttcaggt
     3721 cctggatcgt cgccatccag ccaaccccga ccctggcgat cgggagatcg gggtcgccga
     3781 tcttcacggt atcgaccgtg ctcttcgggt cgacccagga cgctaccgac agcaggtggt
     3841 ttcgaatatc gttggtggtc accagcgtgt cctccttcgg agccgcgggg cggccccaac
     3901 gaaaaaggcc tcccctgggg gaggcctccg tatcttccga acg
//
LOCUS       DASRZS010000259         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_917621, whole genome shotgun sequence.
ACCESSION   DASRZS010000259 DASRZS010000000
VERSION     DASRZS010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917621"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VFH83_06430"
     CDS             complement(<1..1039)
                     /locus_tag="VFH83_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6486036.1"
                     /translation="MKRTVVGLSAFALVVAVLLLGVPIAVMAAPVNITVWDYIDPSKL
                     QGTDAFQLQLQKEFDQKFPNIKVTHAQIPAVSSNDQWPVAITTDAASGQGATIYGQAL
                     NSSMAVGLGVAGVLRDLSPYVNSWADWKDVYDSMKRDVTQAGTSHVYSVPCWGVDVVL
                     LAYRTDFAKAAGLDVTKGPQTWEDLRAWAKKLTDDKTNRAGFSVLADSISDWWFEYFV
                     WQAGGDLTTVNSKGQITLNLTSQPVIDAINFYRTLAYTDGSTQKNPLEGFGDLLNDWY
                     QDRLAMTIFYPSWISWQVPAGLKLDKVNLAVLPRNKTSATAFSMQTYAISSKATQPQA
                     DAAFEYMKYMLG"
     gene            complement(1098..1547)
                     /locus_tag="VFH83_06435"
     CDS             complement(1098..1547)
                     /locus_tag="VFH83_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486037.1"
                     /translation="MRVVAHYYQQFDADPTRDAPAQTVGGWKSDVIEIAEERTALVVM
                     HAWDTGTAEAYPGWYRAVDYLPRANRIGREVLPGLLAAVRASRMRPFHVVGGGEYYKD
                     FPGYLRAVELAGPRVPPLPRIDADPTLNALRRFREQRFREALRQRFP"
     gene            complement(1554..>2290)
                     /locus_tag="VFH83_06440"
     CDS             complement(1554..>2290)
                     /locus_tag="VFH83_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET6486038.1"
                     /translation="DLGYDMALVSLGALRDDAEDALAAHCKAVAEIMPICGFYLQRAV
                     GGRRLSYRFWRRFAEIENVVAVKIAPFDRYATIDVVRAIAESGREEIALYTGNDDAIV
                     ADLLARFRFVVSGPAGERRTVERRIVGGLLGHWSVWTRAAVELLERCRAVQGDPVPAD
                     LMRLGPEVTDANAAFFDAANGYAGCIAGLHEVLRRQGLFEGIWCLDPLETLSDGQAEE
                     IDRVYAAYPHLNDDAFVASNRDAWLA"
BASE COUNT          397 a          773 c          726 g          394 t
ORIGIN      
        1 tgcccagcat gtacttcatg tattcgaacg cggcgtccgc ctggggctgc gtggccttgg
       61 agctgatggc gtaggtctgc atggagaacg cggtggcgga ggtcttgttt cggggcagga
      121 ccgccaggtt caccttatcc agcttgaggc cggctggaac ctgccacgag atccagcttg
      181 ggtagaagat ggtcatggca agacggtcct ggtaccagtc gttgagcagg tcgccgaacc
      241 cctccagggg attcttctgg gtagagccgt ccgtgtaggc cagggtccgg tagaagttga
      301 tggcgtcgat gacgggttgg gaggtgaggt tcagggtgat ctgacccttg ctgttgaccg
      361 tggtcaggtc gccaccggcc tgccagacga agtattcgaa ccaccagtcg ctgatggaat
      421 ccgcgaggac gctgaagccg gcccggttgg tcttgtcgtc cgtcagcttc ttggcccacg
      481 cgcggaggtc ctcccaggtc tggggtccct tggtgacgtc cagccccgcg gccttggcaa
      541 aatccgtccg ataggcgagc agcacaacgt ccactcccca gcagggcacg gagtatacgt
      601 gggaagtccc ggcctgggtt acgtccctct tcatgctgtc atagacgtcc ttccagtccg
      661 cccaggagtt gacgtacggg gagaggtccc gaaggacgcc cgccacgccc aaccccaccg
      721 ccatcgagga gttgagcgcc tgaccgtaga tcgtggcgcc ctgaccggag gccgcgtccg
      781 tggtgatggc caccggccac tggtcgttgg aagacaccgc cgggatctgg gcgtgggtga
      841 ccttgatgtt cgggaacttc tggtcgaact ctttctgcaa ctggagctgg aacgcatccg
      901 tcccttgaag cttgctggga tcgatgtagt cccagacggt gatgttcacc ggcgcggcca
      961 tcaccgcgat gggcacgccg aggagcagca cggcaaccac gagcgcaaac gcgctcaacc
     1021 cgaccaccgt ccgtttcatg tgcatcgcac ctcctttgaa agagatgcgc ggattgtaga
     1081 cccgcgtcac ggaggagtca aggaaaacgt tgccgaagcg cctcccggaa ccgttgttcg
     1141 cggaacctgc gcagcgcatt cagggtggga tccgcatcga tccggggcag aggcgggacg
     1201 cgaggaccag caagctccac ggctcgcaga tacccgggga aatccttgta gtactcccca
     1261 ccccccacca cgtggaacgg cctcattcga gacgcccgca cggcggcgag caggcccgga
     1321 aggacctccc ttccgatccg gttcgcgcgc ggcagatagt ccacggcgcg gtaccagccc
     1381 ggatacgcct cggcggtccc ggtatcccag gcatgcatca ccacgagcgc cgtgcgttcc
     1441 tccgcgatct cgatgacgtc gctcttccac ccgccgacgg tctgcgccgg cgcatcccgc
     1501 gtcgggtccg catcgaactg ctggtagtag tgggcgacta ctcgcatcgc tatttacgcc
     1561 agccaggcgt cgcggttgct cgccacgaac gcgtcgtcat tcaggtgcgg gtaggcggcg
     1621 tagacccggt cgatctcctc cgcctgccca tcactgaggg tctccagcgg atcaaggcac
     1681 cagatcccct cgaagagccc ctggcggcgc agcacctcgt gcagaccggc gatgcagccc
     1741 gcatacccat tcgccgcatc gaagaacgct gcgttggcgt cggtcacttc cggccccagc
     1801 cgcatcaggt cggcgggaac cgggtcccct tggaccgcgc ggcatcgctc cagcagctcc
     1861 acggccgcgc gggtccacac ggaccagtga cccaggaggc cgcccacgat ccttcgctcc
     1921 acggtgcgcc gttctcccgc ggggcccgac accacgaacc ggaaccgcgc caggagatca
     1981 gccactatgg cgtcgtcgtt gccggtgtac agggcgatct cctccctgcc cgactccgcg
     2041 attgctcgaa ccacgtcgat ggtcgcgtac cggtcgaacg gagcgatctt tacggccacc
     2101 acgttctcga tctccgcgaa gcggcgccag aaccggtagg agagccggcg cccgcccacc
     2161 gcccgctgga gatagaaccc gcagatgggc atgatctccg ccacggcctt gcagtgcgcc
     2221 gccagcgcgt cctccgcgtc atcacgaagc gcacccaggc tgaccagcgc catgtcgtat
     2281 ccgaggtccg
//
LOCUS       DASRZS010000260         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2839276, whole genome shotgun sequence.
ACCESSION   DASRZS010000260 DASRZS010000000
VERSION     DASRZS010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2839276"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2181
                     /gene="metE"
                     /locus_tag="VFH83_06445"
     CDS             <1..2181
                     /gene="metE"
                     /locus_tag="VFH83_06445"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET6486039.1"
                     /translation="LQRAAGIDVVPCGDFSLYDHVLDTAVMLGVVPHRFGAPDASLAG
                     YFTMARGASGGGREISPLEMTKWFDTNYHYLVPELSPGQGFRLNPQKLVAEWSEARAL
                     KIPARPVVLGPVTFLSLAKQTEGADGTLSHLDRVLPAYAELLRHLAAQGADWVQIDEP
                     ILVMDLEATAREAFRKAYQALGNSSPAKLLLTTYFGDLRDNLDLALKLPVAGLHLDLV
                     RGPTQLDAALRGLPAQMSLSLGVVDGRNIWRTDLEKALGIVEKAAQTLGSDRVWVSSS
                     CSLLHSPEDLTEETGLDPELKSWLAFARQKLAEIAVLARGANHGRSAIQAELEESTRA
                     RESRRASPRTSNPAVRQRLAGLGPQMSRRASRYPQRREAQSRVLKLPLFPSTTIGSFP
                     QTHEVRAARAGLRSGKLTLAEYEDFLRKEIERTVRLQESLGLDVLVHGEFERNDMVEY
                     FGEMLAGFAFTRNGWVQSYGSRCVKPPVIYGDVERTQPMTVRWSSYAQSLTDRPMKGM
                     LTGPVTILAWSFVRDDQPREATCRQIALAIRDEVQDLEKAGIKVIQIDEPALREGLPL
                     RHADWSGYLGWAVESFRIASSGVRDETQIHTHMCYSEFNEIVPSIVAMDADVISIESA
                     RSRMELLGAFRQFNYPNAIGPGVYDIHSPRVPSTQEVVGLLQKAVEVLAPEQLWVNPD
                     CGLKTRQWSEAEPAVRALTEAARRMREQHAGNGSRPPKAEAAVR"
     gene            2260..>2608
                     /locus_tag="VFH83_06450"
     CDS             2260..>2608
                     /locus_tag="VFH83_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET6486040.1"
                     /translation="MGTMLYEKGVFLNRCFEEVCLSDPNLVLGIHREYLDAGAEVLTT
                     NSWGANLPKLTSRGLAERFAEINEKAVALARKAAEDAGRAGSVHVAGSIGPLGVKLAP
                     LGNLSVPDAEDLFQ"
BASE COUNT          425 a          885 c          883 g          415 t
ORIGIN      
        1 ctgcagcggg cggcgggcat cgacgtcgtc ccgtgcggtg acttctcgct ctacgaccac
       61 gtcctggaca ccgccgtgat gctgggagtg gtcccgcacc ggttcggcgc gccggatgcg
      121 tccctggccg gctacttcac gatggcgcgc ggggcctccg gcggagggcg ggagatctct
      181 ccgctggaga tgaccaagtg gttcgacacc aactaccatt acctggtccc cgagctgtcg
      241 cccggacagg gattccgtct caatccgcag aagctcgttg ccgaatggtc ggaggcgcgg
      301 gcgctcaaga tcccggcgcg ccccgtggtg ctgggcccgg tgacgttcct gtccctggcc
      361 aagcagacgg aaggagcgga cggcacgctt tcccacctgg accgggtgct gccggcgtac
      421 gctgagctgc ttcgccacct ggccgcccag ggcgcggact gggtccagat cgacgagcca
      481 atcctggtga tggacctgga ggcgacggca cgggaggctt tccgcaaggc atatcaggct
      541 ctgggaaact ccagcccggc caagcttctt ctcacgacct acttcggcga tctacgggac
      601 aacctggacc tggctctcaa gcttcccgtg gcaggcctcc acctggacct ggttcgaggg
      661 ccgacgcagc tcgacgcggc gctccggggg ctgcccgccc agatgtccct gtcgctgggc
      721 gtggtggatg ggcgcaacat ctggaggacg gatctggaga aggccctggg catcgtggag
      781 aaggccgcgc agactttggg gagcgatcga gtctgggtct cctcctcgtg ctctctcctg
      841 cactcccccg aggacctcac cgaggagacg ggtctggatc ccgagctcaa gagctggctg
      901 gcgttcgctc gacagaagct ggcggagatc gccgtcctgg cgcgcggcgc caaccacggc
      961 cgatccgcca tccaggcgga gctggaggag agtacccggg cccgagagtc ccgccgcgct
     1021 tctccccgga cgtcgaaccc ggcggtccgc cagcgcctcg ccgggcttgg cccgcagatg
     1081 tcccgccgcg ccagccgcta cccccagcgc cgagaagccc aaagccgcgt gctcaagctg
     1141 ccgctcttcc cgtccaccac gatcggctcc ttcccgcaga cccacgaggt cagggcggcg
     1201 cgcgccggct tgcggtccgg caagctcacc ctggccgagt acgaggattt cctccgcaag
     1261 gagatcgagc gcaccgtccg gctccaggaa tccctcggtc tggatgtgct ggtgcacggg
     1321 gagttcgagc gcaacgacat ggtggagtac ttcggggaga tgctggccgg cttcgcgttc
     1381 acccgcaacg gctgggtcca gagctatggc tcccggtgcg taaagcctcc cgtcatctac
     1441 ggggacgtgg agcgaactca gcccatgacg gtccgatggt cttcctacgc ccagtccctg
     1501 acggatcggc caatgaaggg catgctcacc ggaccggtga ccattctggc ctggtccttt
     1561 gtccgggacg accagccccg ggaggccacc tgccggcaga tcgccctcgc cattcgggat
     1621 gaggtccagg acctcgagaa ggccgggatc aaggtcatcc agatcgacga gccggccctg
     1681 cgggaaggtc tcccgttgcg ccacgcggac tggagcggct acctgggatg ggcggtggag
     1741 tccttccgaa tcgcgtccag cggcgtgcgc gacgaaacgc agatccacac gcacatgtgc
     1801 tactccgagt tcaacgagat cgtgccctcc atcgtagcca tggacgctga cgtgatctcc
     1861 atcgaatccg cgcggtcacg gatggagctg ctgggggctt tccgtcagtt caactatccc
     1921 aacgccattg gccccggagt ctatgacatc cactcgccac gggttccctc cacgcaggaa
     1981 gtggtgggcc tcctccagaa agcggtggag gtgctggcac ccgagcagct ctgggtcaat
     2041 ccggactgcg ggctgaagac gcggcagtgg agcgaggcgg agcctgctgt ccgggcgctc
     2101 acggaggccg cgcgccgcat gcgggagcag cacgcgggca acgggtcccg gccgccgaag
     2161 gccgaagcag ccgtgcgctg atcggccagg ggggacgcca cgccagagga cttccgcgcc
     2221 cgactggaag ggacctccgt catcctcttc gacggagcca tgggcaccat gctctatgag
     2281 aagggggtgt tcctcaatcg gtgcttcgaa gaggtatgcc tgtccgatcc caacctcgtg
     2341 ctcggcatcc atcgggagta cctggacgcc ggcgcggagg tcctgaccac caatagctgg
     2401 ggcgccaatc tccccaagct gaccagccgc gggctggcgg aacggttcgc cgagatcaac
     2461 gagaaggcgg tggctcttgc ccgcaaggcc gcggaggacg cgggccgggc cggctcggtc
     2521 cacgtggccg gctccatcgg ccccctcgga gtgaagctgg cgccgctggg aaacctgagc
     2581 gtcccggacg cggaggacct cttccaga
//
LOCUS       DASRZS010000261         3819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2069376, whole genome shotgun sequence.
ACCESSION   DASRZS010000261 DASRZS010000000
VERSION     DASRZS010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069376"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VFH83_06455"
     CDS             <1..440
                     /locus_tag="VFH83_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486041.1"
                     /translation="GFAFSYLYHIILPLIGAFLGGIGGSLRLLRATMLDQLGQPYITA
                     LRSRGIPEARIYLGHAFRNTLNPFITGSSNLLADLFSGSLILEIIFSYPGIGRLMYEA
                     VLQKDINLVMTNIMFISFLILIGMLLSDVALALVDPRIRYTHA"
     gene            437..1519
                     /locus_tag="VFH83_06460"
     CDS             437..1519
                     /locus_tag="VFH83_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486042.1"
                     /translation="MRRFWFMLIHRPIALASVIILAALYAGMIFCEFLAPYGPNEQFV
                     GHSDEPPNLIWYSAVLGFRPQVQTLVLVNEITRQYAALQGQYTAVTALAIGPDVRLWA
                     FLPIRRHLFGTPLFSNPGSNAEPVFLFGTDNLGRDIFSRILYGSRISLTVGFVGVAIS
                     LVLALLLGGLAGYLGGFWDWITMRITEFIILIPGLYLILFLRSILSTNLTSGQSYALI
                     TVILSFVGWPGTARVIRGMVHAIKRNDFVANAELESMPPLAILFTHIVPQMSSILIVS
                     VTLGVPGFILGETVLSYLGLGITDPAVSWGSMINRDATTISNLVSFPWLLAPGGFLLA
                     VTVSFNFLGELLRDVVDPNWKERLRG"
     gene            1512..2546
                     /locus_tag="VFH83_06465"
     CDS             1512..2546
                     /locus_tag="VFH83_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486043.1"
                     /translation="MDDGQGRSAPVLSAQGLSVSFPTAAGRLYAVRQVDFEIRRGESL
                     GIVGESGSGKSVTASALLGLLGKDAVVGGRVRLEDVEVFDLPPDRLRGIRGTRMSMIF
                     QEPSRSFDPISSIGKAFEETLRVADPSLSREACRDRACRLLTEVHIPRAEERLRNFPH
                     QFSGGMLQRIMIALALADNPQVLIADEPTTALDVTIQSQIIALLKELQTERGLALLFI
                     SHNLALVGQIADRILVMYGGLVLEEGSVEEVLNHPRSPYTRALLDALPGWGSHYSRGR
                     LSTIPGSVPDPTAPEPGCPFAPRCPLVTEQCRTSVPVLVREQGGRQVEVGPEERSAGS
                     VYRCIVAGVK"
     gene            2543..3613
                     /locus_tag="VFH83_06470"
     CDS             2543..3613
                     /locus_tag="VFH83_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486044.1"
                     /translation="MTAPSLSRTEGRSALQIRVRDLAKVYKLEAGIFASAQRRVWAVN
                     GVSFDIRAGETYGLVGESGSGKTTIARMLVQLVEQTAGTIEYYPKESRSSEVTESRDL
                     PPPPWGVHRNDRRLLRRYRSEVKYIFQDPSSALDPRMSAFRILSSGSRFGDRENDSPR
                     PPRAELRERATDVLRAVGLREADLDRRPSEFSGGQRQRLSLARALFSRPTTLICDEVV
                     SALDVSVQGQILNLLVDLKRALSLTILFIAHDLTVVSYMADRVCVLYGGSVMEEAPSA
                     SLVQRPLHPYTQLLYRTIPRLGGMPPDRDQTAAATFNPTRAPVGCPFVGRCPREEAVC
                     RTERPELRVVAPEHRVACHVVT"
BASE COUNT          622 a         1266 c         1184 g          747 t
ORIGIN      
        1 tcgggtttgc cttctcctat ctctatcaca tcattttgcc gttgatcgga gctttcctgg
       61 gcggcatcgg cggctccctg cgcctccttc gagcaacgat gctggaccag ctcggtcagc
      121 cctacatcac cgccctgaga tcccgcggga tcccggaggc ccggatctac ctgggccatg
      181 cattccgcaa caccctcaac ccgttcatca cgggatcctc caacctcctg gcggacctgt
      241 tctccggatc gctcatcctg gagatcatct tctcctaccc cggcatagga cggttgatgt
      301 acgaagcggt gcttcagaag gacatcaacc tcgtgatgac gaacatcatg ttcatcagct
      361 tcctcatcct catcgggatg ctgctgtcgg acgtggcgtt ggccctggta gacccacgca
      421 tacggtacac gcacgcatga gacgattctg gttcatgctt atccaccgac ccatcgcgct
      481 ggcgtccgtc atcatcctgg cggcgctcta tgcggggatg atcttctgcg agttcctggc
      541 accttacggg cccaacgagc agttcgtggg gcattccgat gaaccgccaa acctgatctg
      601 gtactctgcc gtgctggggt tccgccctca ggtccagacc ctcgtcctgg tcaacgagat
      661 cacccgacag tacgccgccc tccagggcca gtacacggcg gtcacggccc ttgccatcgg
      721 acccgacgtc cggctctggg ctttcctccc gattcgccga catctctttg gaacgcccct
      781 gttctcgaat ccgggcagca acgccgagcc ggtcttcctc ttcggcacgg acaacctggg
      841 cagagacatc ttcagccgga tcctctacgg ttcccgcatt tccctcacgg tggggttcgt
      901 gggagtcgcc atctcgctgg tgttggctct cctcctgggt ggacttgcgg ggtatctggg
      961 cgggttctgg gattggatca cgatgcggat caccgagttc atcatcctga tcccgggact
     1021 ctacctcatc ctgttcctgc gctcgattct cagcaccaac ctcacctcgg gtcagtccta
     1081 cgcgctcatc accgtcatcc tctccttcgt gggatggccc ggcacggcgc gggtgatccg
     1141 agggatggta catgccatca agcggaacga tttcgtggcg aacgcggagc tggaatccat
     1201 gccgcccctg gccatcctct tcacccacat cgtgccccag atgtcgtcca tcctcatcgt
     1261 gagcgttacc ctgggggtcc ccggcttcat cctcggcgag acggtgctct cctacctggg
     1321 cctggggata acagaccccg cagtgagctg gggcagcatg atcaatcggg atgcaaccac
     1381 catctcgaac ctggtctcct tcccatggct cctggcgccc ggaggtttcc ttcttgcggt
     1441 gacggtatcc ttcaactttc tgggggagct tcttcgggac gtggtggacc cgaactggaa
     1501 ggagcgcctc cgtggatgac ggtcagggac gttccgcccc ggtcctgagc gcgcaggggc
     1561 tttccgtgag cttccccacg gctgcgggcc gcctgtacgc ggtccgccag gtggacttcg
     1621 agatccgtcg aggggagtcc ctgggcatcg tgggggagtc cggctcgggc aaaagcgtta
     1681 ccgcttcggc ccttctcggc ctcctgggga aggacgccgt ggtgggcggc agggtccggt
     1741 tggaggacgt cgaggtcttc gacctgccgc cggacagact gcgtggcatc cgaggaaccc
     1801 gcatgtccat gatcttccag gagcccagcc ggtccttcga tcccatcagc agcattggca
     1861 aggcgttcga ggaaacgctt cgcgttgccg acccttccct ttcgagagag gcgtgccggg
     1921 accgggcctg caggctcctc accgaagtcc acattccgcg ggccgaggag cgcttgcgaa
     1981 actttccaca ccagttctcg gggggcatgc tgcagcggat catgatcgcc ctcgctctgg
     2041 cggacaatcc ccaggtcctc atcgcggatg aaccgacgac agccctggac gtcaccatcc
     2101 agtcgcagat catcgcgctg ctcaaggagc ttcagaccga gcgaggtctg gcgctcctct
     2161 tcatctccca caacctggcc ctggtagggc agatcgccga tcggatcctc gtgatgtacg
     2221 gaggactggt cctggaagag ggttccgtgg aagaggtgct gaaccacccg agaagtcctt
     2281 atacccgcgc actgcttgac gctctccccg ggtggggcag tcactactcc cggggtcgcc
     2341 tctccacgat ccccggttcg gtgcccgatc ccacggcacc cgagccgggc tgcccctttg
     2401 cgcctcggtg ccccctggtc accgaacagt gcaggacctc cgtgccggtc ctggtgagag
     2461 aacaaggggg caggcaggtc gaagtcggcc cagaggagcg ctcggcaggc agcgtgtacc
     2521 gatgtatcgt ggcaggggtg aaatgaccgc cccttcgctc tcacggaccg agggacggtc
     2581 cgccctccag atccgcgtgc gcgatctcgc caaggtgtac aagctggagg ctggcatctt
     2641 cgcctcggcc caacgcagag tctgggccgt caacggagtg agcttcgata tccgcgcagg
     2701 ggagacctac gggttggtcg gtgaatcggg atccggaaag acgacgatag ctcggatgct
     2761 cgttcagctg gtggagcaaa ccgcaggaac catcgagtat taccccaagg agagccgcag
     2821 ctcggaggtc acggaaagcc gcgaccttcc ccctccgccg tggggtgttc accgaaacga
     2881 ccgtcgtctg ttgcgacgct atcggtccga ggtcaagtac atcttccagg atccctcttc
     2941 ggcactggac ccgcggatga gtgcctttcg gatcctgtcc tccggatcga gattcggcga
     3001 ccgggagaac gactccccaa gaccgccgcg cgcggagctg cgggaacggg caacggacgt
     3061 gctgcgcgcc gtcgggcttc gcgaggcgga cctggatcgg cgtccgtcgg agttctccgg
     3121 tggacagcgc cagaggctct ccctggcgcg cgcgctgttc tcccgcccga ccacgctgat
     3181 ctgtgacgag gtggtaagcg ctctggacgt gtctgtgcag ggtcagatcc tgaacctgct
     3241 ggttgacctg aagcgggcgc tcagcctgac gatcctgttc atcgctcacg accttaccgt
     3301 ggtctcttac atggcagacc gggtgtgcgt cctttatggc gggagcgtca tggaggaagc
     3361 cccgtccgcc agcctggtgc agaggccgct tcacccgtac acgcagcttc tctaccgtac
     3421 catcccgagg ctcggaggca tgcccccgga ccgggatcag accgcggctg ccaccttcaa
     3481 cccgacccgc gcaccggtag gctgcccctt cgtgggcagg tgcccgcggg aagaggccgt
     3541 ctgccgcacg gagaggccgg agcttcgagt ggttgcccca gagcatcggg tggcctgcca
     3601 tgtcgtgacc tgaagcggat agcctggggg agctgcccgc cagggagtca acggccgccg
     3661 tagcgaagcg cggtctccag gtagcgcagg gcgttcgcgt tgaaccgttc cgtatgctgc
     3721 tggtggggcg tcgcggaggg agccagaacg aagcgggggc cgccctcgtc gatagcgctc
     3781 cgtacggccc gctcgatctc agaggggctg gccatgtcc
//
LOCUS       DASRZS010000262         3894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_918703, whole genome shotgun sequence.
ACCESSION   DASRZS010000262 DASRZS010000000
VERSION     DASRZS010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918703"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VFH83_06475"
     CDS             complement(<1..451)
                     /locus_tag="VFH83_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486045.1"
                     /translation="MAETLTSRTITATYSVTDGALRAESIQNRLTGRTVPLVSSSEVA
                     VVLSSVVDRVAEPRRVDDFRVVETVRRQDAGVVWLLRSADSGLEVEVHVDLQDSTRIK
                     WVELINPGQREVLVLDVVVDDFRLDTALEGGGQGQPAFIASELFAAIE"
     gene            complement(489..2318)
                     /locus_tag="VFH83_06480"
     CDS             complement(489..2318)
                     /locus_tag="VFH83_06480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HET6486046.1"
                     /translation="MTTEKLPHWDLRNVYPSLDSAELAADTAALASTLDEIDRNLAAW
                     IAEPDTASTVSRLDTGVELFNRALLLSSTLRIFVESFVTTNSYDTAAAKKLSELEMVQ
                     VRLQQSTVRLQGWLRGLGKTLPEALKPAGGAGGATGPVGALARDHAFILLEAAEQGRY
                     LMSEPEELLSAELSLTGANAWSKLQGTVTSQIVVDFELDGVTRPMPITAIVNLRTHPE
                     EAVRHRAYDVEMREWHKVREPLAACMNGIKGVANTLWKRRGRMDCLHASLDQGRIDRA
                     TLEALLDAMRGSLPIFRRYFAAKARRIGKERLAWWDLFAPSGRASQTYSFSQARELVL
                     ETFGRFASDLRSFAEQAFARSWIDAEPRDGKRGGAFCAAVPRVKESRILCNFDGSLEQ
                     VSTIAHELGHGYHNHCAYAASRTELQQSTPMTLAETASILCEQIVNDALLARATDPQE
                     RLGILENALINDSQVIVDIYSRFLFEKEVLERRERSELSADDLCQIMERAQAAAYGEG
                     LDERYRHRYMWTWKPHYYIPELSFYNYPYAFGLLFGTGLYARFLSQGAGFIPDYQKLL
                     AGTGEAPAAELANRFGIDITRRTFWDGSLAIIARRVEDYCSIS"
     gene            complement(2315..3418)
                     /locus_tag="VFH83_06485"
     CDS             complement(2315..3418)
                     /locus_tag="VFH83_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HET6486047.1"
                     /translation="MSADTRIHLRESFFDFTERLLLAHGELEARAFRWDTGVAGLRIS
                     NARGSMVVLPFQGQQVWDAAFDGRTLTMKSMVDRPYPTREYLATYGGFVIHCGVTAMG
                     VPTKEDSHPLHGELPNAPYATAFIEAGTDNGGPYLAVGGEYLHVVAFNHKYLARPLAR
                     LRAGSTMIEVALEVENLKRTPMEILYLAHVNFRPVDNGKLVFSAPCTAQTVRVRTSIP
                     PHIHPPAGFAEFLQDLARHPERHCVLSPGLAFDPEVVLYLDYRADAQGWARSLLVHPD
                     GYASCIRHRPSQLDHGVRWISRTPDQDCLGIVLPATAEPEGYTAEKRKGNVKTLAPGA
                     TVRWELEAGLLAPREAQEEERAIQRILAGTAAS"
     gene            complement(3440..>3894)
                     /locus_tag="VFH83_06490"
     CDS             complement(3440..>3894)
                     /locus_tag="VFH83_06490"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HET6486048.1"
                     /translation="PGELLEWLNRRLCEKLSASPDLLVTFGACLLDTAACTMSYANAG
                     HVPIHVVRGDTVTTLRAQGIGLGFDEEGHFPAQRYDLHSGDIIVLHTDGLQELSRGTL
                     LGDEAFGQVLVHSAKADDFHQAVLTELRGHTEGGLFSDDATLLSARLR"
BASE COUNT          616 a         1309 c         1312 g          657 t
ORIGIN      
        1 gctcgatggc cgcgaagagc tccgaggcga tgaacgccgg ttgcccctgg ccgccgcctt
       61 ccaacgcggt gtcgagccgg aagtcgtcca ccacgacgtc caggaccagg acctcccgct
      121 ggcccggatt gatgagctcc acccacttga tccgggtgga gtcctgcagg tcgacgtgga
      181 cctccacctc cagtcccgag tcggcgctcc gcagcagcca gacgacccca gcgtcctgcc
      241 gccgcaccgt ttccacgacg cggaagtcat cgacacgccg aggctccgcc acccggtcca
      301 cgacggagga gagcacgacc gccacctccg acgatgacac caaggggacg gtccgtccgg
      361 tgagccggtt ctggatgctc tcggcgcgca acgcgccgtc cgtcaccgag taggtggccg
      421 tgatggtcct gctggtcagg gtctccgcca tcttcgtccc tgcctttctg agcgctcttg
      481 ctcggatatc aagagatgct gcagtagtct tccacgcgcc gcgcgatgat cgccaggctg
      541 ccgtcccaga aggtccggcg ggtgatgtcg attccgaacc gattggccag ctccgccgcg
      601 ggcgcctccc cggttcccgc cagcagcttc tggtagtccg ggatgaagcc ggcgccctgc
      661 gataggaacc gcgcgtacag ccccgttccg aagagcagac cgaacgcgta cgggtagttg
      721 tagaagctca gctccggaat gtagtagtgg ggtttccagg tccacatgta gcgatgtcga
      781 tagcgctcat ccagaccctc tccgtaggcc gccgcctggg cgcgctccat gatctgacag
      841 aggtcgtccg ccgagagctc ggagcgttcc cggcgctcca ggacctcttt ctcgaagagg
      901 aaccgcgagt agatgtcgac gatgacctgg gagtcgttga tcagggcgtt ttccaggatg
      961 ccgaggcgct cctgggggtc cgtggcccgg gccaggagcg cgtcgttgac gatctgctcg
     1021 cagaggatgg aggcggtctc cgcgagggtc atcggcgtcg actgctggag ctcggtccgg
     1081 gaggcggcgt acgcgcagtg gttgtgatag ccatgaccca gctcgtgcgc gatggtggac
     1141 acctgctcca ggcttccgtc gaagttgcag aggatccgcg actccttgac cctgggcacc
     1201 gcggcgcaga aagccccgcc gcgcttcccg tctcggggct ccgcgtcgat ccaactccgg
     1261 gcgaacgcct gttcggcgaa ggagcgaagg tccgaggcga accgcccgaa ggtctccagc
     1321 accagctcgc gggcctggga gaagctgtac gtttgggagg cgcggcccga tggggcaaag
     1381 aggtcccacc acgccagccg ctcctttccg atccgtcggg ccttggcggc gaagtaacgc
     1441 cggaagatcg gaaggcttcc ccgcatggca tccaggagcg cctccagcgt ggcccggtcg
     1501 atcctgccct ggtccaggga cgcgtgcaga cagtccatcc gtccgcgccg cttccacagg
     1561 gtgtttgcca cccctttgat cccgttcatg caggcggcca ggggctctcg gaccttgtgc
     1621 cactcccgca tctccacgtc gtaggcccgg tggcggaccg cctcctccgg atgcgtgcga
     1681 aggttcacga tggccgtgat gggcatgggg cgcgtgacgc cgtccagctc gaagtccacc
     1741 acgatctgcg aggtcacggt cccctggagc ttgctccagg cgttggcgcc cgtcaggctc
     1801 agctccgcgc tgaggagctc ttccggctcg ctcatgaggt agcgcccctg ctcggccgcc
     1861 tccaggagga tgaaggcatg gtcccgggcc agagcgccca ccgggccggt cgcgcctccc
     1921 gccccgcccg cgggcttcag agcttccggc agcgtcttcc ccagaccccg gagccatccc
     1981 tggagccgga ccgtggattg ctgcagacgc acctgcacca tctccagctc ggagagcttc
     2041 ttcgcggcgg ccgtgtcgta cgagttggtg gttacgaagg actccacgaa gatgcgcagc
     2101 gtagaggaga gaagcagagc gcggttgaag agctccacgc cggtgtcgag ccgggagacc
     2161 gtggaagccg tgtccggctc cgcgatccag gcagccaggt tgcggtcgat ctcatccagc
     2221 gtcgaggcca gcgccgcggt gtccgccgca agctcggcgg aatccagcga cgggtagacg
     2281 ttccgaagat cccagtgagg aagtttttcc gtggtcatga tgccgcggtg cctgccagga
     2341 tgcgctggat agctcgttcc tcttcctgcg cctctcgagg agccagcagc ccggcctcca
     2401 gctcccatcg gacggtggcg ccgggcgcaa gggtcttcac gtttcctttg cgtttctccg
     2461 ccgtatagcc ctccggttcc gccgtggccg gcagcacgat ccccaggcag tcctggtccg
     2521 gagtgcggct gatccagcgc acgccatggt cgagctgaga cggcctgtgc cggatgcagc
     2581 tcgcgtatcc gtccggatgg accaggaggc tgcgcgccca cccctgagcg tccgcccggt
     2641 agtccaggta aaggaccacc tccgggtcga acgccagccc tggagagagg acgcagtggc
     2701 gctccggatg acgcgccagg tcctggagaa actcggcgaa ccccgccggc gggtggatgt
     2761 gcggagggat ggacgtgcgg acgcgcaccg tctgcgcggt gcacggggcg ctgaatacca
     2821 gcttgccgtt gtccacgggg cggaagttga cgtgcgccag gtacaggatc tccatgggcg
     2881 tccgcttgag gttctccacc tccagcgcca cctcgatcat ggtcgaaccg gctcgcagcc
     2941 gagccagcgg acgcgccagg tacttgtggt tgaacgccac cacgtgaagg tactccccgc
     3001 ccaccgcgag gtacgggccg ccgttgtcgg tgccggcctc gatgaatgcg gtcgcgtacg
     3061 gggcgttggg gagctccccg tgcaaggggt ggctgtcctc tttggtgggc actcccatgg
     3121 cggtcacccc acagtggatg acgaaccctc cgtaggtggc caggtattcc cgggtggggt
     3181 aaggccggtc caccatggat ttcatggtca gggtccgtcc gtcgaacgcc gcgtcccaga
     3241 cctgttgtcc ctggaagggc aggacgacca tggacccccg ggcattgctg atccgtaacc
     3301 ccgccacgcc ggtatcccaa cggaacgcgc gggcctcgag ctctccgtgg gccaggagga
     3361 gcctctcggt gaaatcgaag aacgactctc tcagatggat cctggtgtcc gcgctcatgg
     3421 ggcctccttc ggtccgcggc taccgcagcc gcgcgctgag cagggtcgcg tcgtcggaga
     3481 acaagccgcc ttccgtatgg ccgcgcagct cggtgaggac ggcctggtga aagtcgtccg
     3541 cctttgccga gtgcaccagg acctgcccga acgcctcgtc accgagcaag gtgccgcggg
     3601 aaagctcctg caggccgtcc gtatggagaa cgatgatgtc tccggagtga aggtcgtacc
     3661 gctgggcggg aaagtggcct tcctcgtcga accccaggcc gatcccctgc gcccgaaggg
     3721 tggtcacggt gtctcctcgg accacgtgga tggggacgtg gccggcgttg gcgtagctca
     3781 tcgtgcacgc cgcggtgtcc aggaggcacg cgccgaaggt caccagcaga tccggagagg
     3841 cggagagctt ctcgcagagc cgccggttga gccactccag gagctccccg gggt
//
LOCUS       DASRZS010000263         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5537771, whole genome shotgun sequence.
ACCESSION   DASRZS010000263 DASRZS010000000
VERSION     DASRZS010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5537771"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VFH83_06495"
     CDS             complement(<1..733)
                     /locus_tag="VFH83_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6486049.1"
                     /translation="MPDSLKDSVIEPGDPTYPQVKSTFFRGGAPGIVFQAKNTGQVVE
                     ATAFARSHAHVPLGIRSGGHGIRGRSTNDGGIVIDLSRMAKIEVLDRSSRLVRVEPGA
                     RWMHVAAALAPLGWAVSSGDSGAVGVGGLATAGGIGLLGRAHGLTIDHLRAVELVLAD
                     GAVVRASDRERADLFWAMRGAGSQFGIATSFEFQAAEVRDVGWAQMVFNAEDIAGLLE
                     KFGAIVESSPRDLTSFLILTPPRLGQ"
     gene            complement(844..1560)
                     /locus_tag="VFH83_06500"
     CDS             complement(844..1560)
                     /locus_tag="VFH83_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET6486050.1"
                     /translation="MSKIVILGATGSLGRHVVQQAVAANHRVSVLTRSEPGVAAGIPE
                     SVVVHRADLSEMSASDLAAILGDHEVVINAAGNVAQGQRFVDLVDHIVSSLEQIPETE
                     RPVAWFLAGLGLLDIDGHGRKGVDVPMIGQNYWPHRSNYDRIRQTDLDWRILCPGPMV
                     DERPLGLTRLRISLDRLPLDIPDKIQSMPDAVLLRFLRDHMSETVVPYQDAAALMLTN
                     IAPGGEMSRHRVGLALPSEG"
BASE COUNT          463 a          887 c          814 g          463 t
ORIGIN      
        1 gctgacccaa gcgcggcgga gtgaggatca ggaagcttgt caggtccctc ggcgagctct
       61 ccacgattgc tccaaacttc tccagcaggc cggcgatgtc ttcggcgttg aagaccatct
      121 gggcccatcc cacgtctctc acctccgcgg cctggaactc gaacgaggtc gcgattccga
      181 actgagagcc agcgcctctc atcgcccaga agagatccgc gcgttcccgg tcactggcac
      241 gtaccaccgc gccgtccgca aggaccagct cgacggcgcg caggtgatcg atcgtcaggc
      301 cgtgtgcgcg tccgagcagg ccgatccccc ccgcggttgc caggccgcca accccgacgg
      361 cgccactgtc accagagctc accgcccatc ccaacggagc cagggcagca gcgacgtgca
      421 tccaacgggc tccgggctcg acgcgaacga gccgggagga ccgatccagg acttcgatct
      481 tcgccattcg ggatagatcg atcacgatgc ctccatcgtt tgtggaccgg ccgcggatgc
      541 cgtgcccccc gcttcggatc cccagcggca catgcgcgtg ggaacgcgcg aacgcggtcg
      601 cctcgacgac ctgccctgtg ttcttcgcct ggaaaacgat gccgggagct ccgccgcgga
      661 agaacgtgga cttgacttgc ggataggtgg gatctcccgg ctcaatgacg ctgtctttga
      721 gcgagtccgg caccgaatcg taggcgattc cagggagccg gttcgcccga tcgtccgggc
      781 gcctcgacgg tccgggatcc tggcccgatg gagacccccg gtaatcttga tggctcatcc
      841 ccgttacccc tccgaaggca gagccaaccc cacgcggtgg cgggacattt cgccgccggg
      901 agcgatgttc gtaagcatca gggcggccgc gtcctggtac ggtacgacgg tttccgacat
      961 gtgatcgcgg aggaaacgca gaaggacggc gtccggcatg gactggatct tgtccggtat
     1021 gtccagggga agtcggtcca acgagatgcg caacctggtg agtcccaggg gacgctcatc
     1081 gaccatgggc cccggacaca ggattctcca atccaggtcg gtctggcgga tacggtcata
     1141 attggagcga tgaggccaat agttctggcc gatcatcggc acgtcgacac cctttcgacc
     1201 gtgcccgtcg atatccagca ggcccaggcc cgccaggaac caggccaccg gcctctcagt
     1261 ctcggggatc tgttcgaggc tggaaacaat gtgatcgacc aggtcgacaa acctttgtcc
     1321 ctgcgcgacg tttccggccg cgttgatcac cacttcgtga tctcccagga tcgctgcgag
     1381 gtcagatgcc gacatctcgg acagatccgc ccggtgcaca acgacgcttt ctggaattcc
     1441 ggccgccacg cccggctcgc ttcgagtcag caccgacacc ctatggtttg ccgcaaccgc
     1501 ctgctggacg acgtgcctcc cgagggagcc cgttgcgccc aggatgacta tctttgacat
     1561 gatcgtggtt cctcctttga agccatgctt ttcagacacc ccattctcag gacacgcggc
     1621 ctagcgcccg attcgccagg tagacagcgg cgccgccaga aacggccgca cgatttcggg
     1681 atcgaattgc gatccggccc gactgcgatg caggacgccg tgtcgaaggc aagaccccgt
     1741 gtcgtcaaca tggcggcgac aaggtcgcgg acctgttcat ggccttccag gagaaggacc
     1801 gccggcccac cggttcgaac cgcggtggct tccgcgctcc ggaccccgcc cctcgcaccg
     1861 acgcttggcg tcatggcctc cccaggttca tcgcttaccc acatcgtatt gctctgcctc
     1921 cctgcaggaa tatcactgaa aatcaccggt gccgtgaacc gtaccaaggt ccagaagtgg
     1981 gaggggtgat cggcgggtac cctgccgggg cggagatgac cgtgagcttg tgcgcggcac
     2041 ctcggtccag ctgctggtag agccgcggcc ggtggaggac gttcttaccg tggactgcat
     2101 cgcctcgcgt cgtgggcagc agcttaccct cttgatatcg aatcaccagg ttccccgttc
     2161 gcccattcgc gcggagcacg tgcacctcca cctccggggc ctcgcggggg ttcgctcggt
     2221 ctccctggag cggattgacg agcagcacgg gaacccgaga agggcctgga tcgagatggg
     2281 cagcccccct actccgaccg cgcctaactc cgcgtgctcg ctgacgcctc ggagctgcgt
     2341 cgggagacgg ttgagtggac acgcgacgag ggcggcgcct cgatcaacct gctggtccca
     2401 ccgcactccg tcgtcggcct ttcgctggaa gcctcatagg tcggcgatcc tcggcccgtg
     2461 catccgcccc tgtttgcttc gacggcgcgt gtgtacacgg cgtccagaga ggcccttgcg
     2521 gggaacgaca tctctgtccg cgaaggaccg tagaaagcgt gcgatgcctc tgggtgaaag
     2581 ttgtacgccc agttcacgag ctaccgcccg gtagcgagga cgacgtc
//
LOCUS       DASRZS010000264         4795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4766550, whole genome shotgun sequence.
ACCESSION   DASRZS010000264 DASRZS010000000
VERSION     DASRZS010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4766550"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFH83_06505"
     CDS             <1..341
                     /locus_tag="VFH83_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486051.1"
                     /translation="ELYRRVFYFEQEKPYFRHFTEEVIDRLRILLCSKMLPTLEPQEA
                     IPLLEELIELDFSRKDNAYFCKKLAEIHASRGDHREALRCLHQGLQWNRKLPGVKKLM
                     EKMERIGHPA"
     gene            497..1552
                     /locus_tag="VFH83_06510"
     CDS             497..1552
                     /locus_tag="VFH83_06510"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6486052.1"
                     /translation="MKRLVAIAAMLCVLAATGWAQVLDKPAATVRLIRTDVITVGQLQ
                     KVVASLQTSAGRLLTQDERKLALNTMVEGALIEQAADRDKIVVSDAELKSAVDGYRKS
                     LATAANLGRDLTDAEFAQYLKNNNISNDEFTKQVRDSRLRLDYVMAKKKGLLDGVKTV
                     TDQEASDYYDAHKSAFFVDDIVVLRHIFVDTRTLTAKDEKDKAAKRADDILKELKAGA
                     SFSDLVLKYSDDAASKYKGGEIGALSRSDTQRRQLFGTAFFDAVFKLKQGETSGVIPS
                     NLGFHIVQVANRYNAQLLSLDDKIPPTNQATVREYIKALILNQRQNDAMTAAASDLVA
                     DLKKQAEIHTYDDNLTW"
     gene            1581..2003
                     /gene="nusB"
                     /locus_tag="VFH83_06515"
     CDS             1581..2003
                     /gene="nusB"
                     /locus_tag="VFH83_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET6486053.1"
                     /translation="MGSRRKGRVIAFQSLYRHDLSGAGLSELLEFSWLDEERTTHPDT
                     LAFARLLIQGALENLPEIDELIKGQLENWDFKRLNRVDLAILRLGVYCLLHQSDIPAT
                     VTIDEAIDISKAFGTEDSYRFVNGVLDGVRKRLAATRI"
     gene            2007..3656
                     /locus_tag="VFH83_06520"
     CDS             2007..3656
                     /locus_tag="VFH83_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486054.1"
                     /translation="MARGTRLFLPVAAAILVAGGVGLFFHFRLPRAVPDEIRAAIGRM
                     DQAIAGGYFSTALDELDRLPALPSDEGTLLSLAKRAFQVSGASGDYAPLARFMERALA
                     ANGSSPRLRLIGAYADLRSGRLAMAQRLVREGLSPRDLGEGIRAEAALRGGQAWNGSD
                     PLARQLLGLEANANPAAFVQAAASTGDARLTLDAALLEMGAGAGSQAQMAARALPDGP
                     AFDEPAALIDYDVGAAGAAVTRIQRALVDRPSAERELLLADALHAAGAPVRAEAALAA
                     AVASQPDLTWTAYANLAYLAAARGALADAARRVAEGLTALPDSQELAVAAARVRVQAG
                     DRQGAAAALSALVEAHPQNVPAAFLLLELRSPAMSPGSYRASLWKLFDRAPYDGRTYA
                     ALLQALVSAQDWAGAAIAVRQHRLATDQTDTATLELEGLVAAMRDDLPSAVASLKAAV
                     GLAADAAARYDLALVLLKEGNTAEARAQLSQAQQEASSVADPARRMRLASRIAAALGM
                     TRVADGDLSGGHADLVRSVSLDPSNLRARLLVMNLEPGGQQ"
     gene            3716..4180
                     /locus_tag="VFH83_06525"
     CDS             3716..4180
                     /locus_tag="VFH83_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET6486055.1"
                     /translation="MPGKNDVTNCIIGEGSIFDGRFYVNGSILIEGKFQGDIKTDDQL
                     IVGPTGKVKTDIVARKVTVAGTLIGNITASEEVSLLHTGKILGNINTPKLTVEPGVVT
                     EGKVTITSGQSEEAKKIIEDSFGTETEEAFSTIIKDRKRDKTRDKEREEPAK"
     gene            4187..>4795
                     /gene="map"
                     /locus_tag="VFH83_06530"
     CDS             4187..>4795
                     /gene="map"
                     /locus_tag="VFH83_06530"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET6486056.1"
                     /translation="MIRLKNDREVDGIRESGRLLARTLQELRPLVVEGVSTRELDSFA
                     RSYIESHGGKPSFLGYMDYPASLCVSVNDEVIHGIPGGRRLKGGDVVSLDLGVNLNGY
                     FSDAAVTVPVGETSRKRRWLLEVTEECLRLAVQQAVPGNRVSDVSRAVFDHAVRNRCG
                     VVREYCGHGVGFSQHEDPQVPNYVGAGPNPRLKPGMVLAIEPM"
BASE COUNT          847 a         1569 c         1607 g          772 t
ORIGIN      
        1 acgagctgta tcgccgggtc ttttatttcg agcaggagaa gccgtacttc cggcacttca
       61 ccgaagaggt catcgaccgg cttcggatcc ttttgtgctc gaagatgctg cccaccctgg
      121 agcctcagga ggccatcccc cttctcgagg agctgatcga gctggatttc tctcgcaagg
      181 acaatgccta tttctgcaag aagctcgcgg agatccatgc ctcacgcggc gaccatcggg
      241 aggcgctgcg ttgcctccac caggggctgc agtggaatcg aaagctgccg ggagtcaaga
      301 agctgatgga gaagatggag aggatcggcc atccggcata gccagacgat gctcgggcgc
      361 cagcggcgtg tccagtctgc gggcggccgg aggccaccgg ttcgtggcgc ggcttgacaa
      421 tccgggtgcc ctccgcttta ctgaacgtgg actgcctcac cacccgtggg cgaaaccaga
      481 ggagcggtat ctcaagatga aaagactggt ggccatcgcg gccatgctat gcgtcctggc
      541 ggcgaccggt tgggctcagg tgctggacaa gcccgcggcc accgtgcgcc tcatccggac
      601 cgatgtgatc acggttgggc agctccagaa ggtcgtggct tccctccaga cctccgccgg
      661 gcgcctgctg acccaggacg agcgcaagct cgccctgaac acgatggtcg agggggcgct
      721 gatcgagcag gccgcggacc gggacaagat cgtggtctcg gacgccgagc tcaagtcggc
      781 tgtggatggt taccgcaagt ccctggccac ggccgcgaac ctgggccgag acctgacgga
      841 cgcggagttc gcgcagtacc tgaagaacaa caacatcagc aacgacgagt tcaccaagca
      901 ggttcgggac agccggctcc gcttggatta cgtgatggcg aagaagaagg gtctcctgga
      961 cggcgtgaag acggtcacgg accaggaggc cagcgactac tacgacgctc acaagagcgc
     1021 cttcttcgtg gacgatatcg tggtgctccg tcacatcttc gtggatacgc ggaccctgac
     1081 ggccaaggat gagaaagaca aggcagccaa gcgggcggac gatatcctta aggagctgaa
     1141 ggccggtgcc tctttctcgg atctcgtgct gaagtactcc gacgatgccg cctccaagta
     1201 caagggcggc gagatcgggg cgctctcgcg gtccgacacg cagcgccgtc agctcttcgg
     1261 gaccgcgttc ttcgacgccg tgttcaagct caagcagggg gagaccagcg gcgtcatccc
     1321 ctccaatctc ggcttccata tcgtccaggt cgccaaccgc tacaacgcgc aacttctcag
     1381 cctggacgac aagatccccc cgaccaacca ggcaacggtg cgggagtaca tcaaggctct
     1441 cattctcaac cagcggcaga acgacgcaat gaccgccgcg gccagcgacc tggtggcgga
     1501 tctgaagaag caggcggaga tccacaccta cgacgacaac ctgacctggt agaccggctg
     1561 ccacggcgtg gcggaatgcc atgggatccc ggaggaaagg cagagtcatc gcgttccagt
     1621 ctctctatcg ccatgacctc tccggcgccg ggctgtccga gctgctggag ttctcctggc
     1681 tggatgagga gcgcaccacc cacccggaca cgctcgcctt cgcgcggctc ctgatccagg
     1741 gcgcgctgga gaatcttccc gagatcgacg agctcatcaa gggccagttg gaaaactggg
     1801 acttcaagcg actgaatcgt gtcgatctgg cgatccttcg cctcggcgtg tattgcctgc
     1861 ttcaccagag cgacatcccc gccacggtca ccatcgacga ggccatcgat atctccaagg
     1921 cctttggcac cgaggattcc taccgattcg tcaacggagt actggacggc gtgcgcaaga
     1981 ggctggcagc caccaggatc tgagaaatgg cgcgcgggac ccgtcttttc ctgccggtcg
     2041 ctgccgccat ccttgtggca ggcggagtgg gtctgttctt ccatttccgc ctgccccgag
     2101 cggttccgga tgagatccgc gctgccatcg gccgcatgga tcaggccatc gcgggaggct
     2161 atttttccac cgctctagac gagctggacc gccttcccgc cctcccatcc gatgagggaa
     2221 cactcctgag cctggccaag cgcgccttcc aggtcagtgg ggcctccgga gactatgcgc
     2281 ccctggcgcg cttcatggaa cgcgccctgg cggccaacgg ctcgagcccg cggctgcgac
     2341 tcatcggagc gtacgctgac ctgcggtccg gccgactcgc catggctcag cgcctggtac
     2401 gggaggggtt gagcccacga gacctcgggg aagggatccg cgcggaggcc gctcttcgag
     2461 gcggtcaggc gtggaacgga tccgatcccc ttgcccggca gcttctcggg cttgaggcga
     2521 acgccaatcc agccgcgttt gtccaggcgg ccgcgagcac gggggacgcc cgcctgactc
     2581 tggacgcggc cctcctggag atgggagccg gcgccggcag ccaggcgcag atggcggccc
     2641 gtgccctccc ggacggcccc gcattcgacg agccggcggc gctcattgac tacgacgtcg
     2701 gggcggcggg cgcggcggtc acccgtatcc agcgcgccct ggtggatcgt cccagcgccg
     2761 agcgggagct gcttctggcg gacgcgctgc atgccgcggg cgcgccggtt cgtgccgaag
     2821 ccgcgctcgc cgccgcggtc gccagccagc cggatctgac ttggacggcc tacgcgaacc
     2881 tggcgtacct cgccgccgcc cgcggcgcgc tggcggacgc ggcccgcagg gtcgccgagg
     2941 ggctgaccgc tctcccggac tcccaggagc tggccgtggc ggcggcgcgg gtccgggtcc
     3001 aggccgggga ccgccagggc gcggccgcgg cgctctccgc tctcgtggag gcccatccgc
     3061 agaacgtgcc ggcagcgttc ctgctcctgg agctccgttc accggcgatg tcgcccggct
     3121 cctatcgggc aagcctctgg aagctcttcg atcgagctcc gtacgacgga cggacctacg
     3181 ccgcgctcct gcaggcgctc gtctccgcgc aggactgggc cggcgcggca atcgcggtac
     3241 ggcagcatcg cctggccacg gatcagacgg acacggccac gctcgagctc gaagggttgg
     3301 tggccgccat gcgggatgac ctgccgtccg cggtcgcttc cctgaaggcc gccgtcggcc
     3361 ttgcggccga tgcggcggcg cgctacgatc tggccctcgt cctcttgaag gaagggaaca
     3421 ccgcggaggc acgggcgcag ctctcccagg cgcagcagga ggcttcctcc gtggccgacc
     3481 cggcgcggcg gatgcgcctg gcgtcgcgga tcgccgcggc gctgggcatg acgagggtgg
     3541 ccgacggcga cctgtccggc ggccacgccg acctcgtgag gtcggtaagc ctggatccct
     3601 ccaatctcag ggccaggctg ctggtgatga atcttgaacc ggggggacaa cagtgatagg
     3661 ttttccacgc gctgcgggcg gccgggccgg cccctccgcg gcgaaaggag agtcgatgcc
     3721 agggaagaat gacgtcacga actgcatcat cggtgaagga tcgatcttcg acggtcgctt
     3781 ctacgtcaac ggctccattc tcatcgaagg aaagttccag ggcgacatca agacggacga
     3841 ccagctcatc gtgggcccga ccggcaaggt gaagacggac atcgtggccc gcaaggtgac
     3901 ggtggccggg acgctgatcg ggaacatcac ggcctcggag gaggtgagcc tcctccatac
     3961 gggcaagatc ctcggcaaca tcaatacgcc caaactgacc gtggagccgg gggtcgtcac
     4021 ggaaggaaag gtgaccatca cctccggtca gtccgaggaa gccaagaaga tcattgagga
     4081 ttccttcggc acggagacgg aggaggcgtt ctccacgatc atcaaggacc gaaagaggga
     4141 caagacgcgt gacaaggagc gggaggagcc cgcgaagtag gtccccatga tccggctcaa
     4201 gaacgatcgc gaggtcgacg ggatccggga atccggccgg cttctcgcca ggactctgca
     4261 ggagctgcgg cccctggtcg tggagggggt ctccacgcgg gagctggact cctttgctcg
     4321 ttcctatatc gaatcccacg gcgggaagcc ctcgttcctg ggctacatgg actatcccgc
     4381 ttccctctgc gtctcggtca acgacgaggt catccacggc atccccgggg gcagacgcct
     4441 caagggagga gacgtggtga gcctggacct gggcgtgaat ctcaacgggt acttctccga
     4501 cgccgccgtc acggttcccg tcggcgagac gtcccgaaag cgccggtggc tgctggaggt
     4561 gaccgaagag tgcctgaggc tggccgtcca gcaggccgtg cccggcaacc gggtcagtga
     4621 tgtctcccgt gccgtcttcg atcacgcggt gcgcaaccgc tgcggcgtgg tccgcgagta
     4681 ctgcgggcac ggcgtcggct tttcccagca tgaggatccc caggtcccga actacgtggg
     4741 ggcgggcccc aacccgcggc tgaagcccgg catggtgctg gccatcgagc cgatg
//
LOCUS       DASRZS010000265         4778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5150649, whole genome shotgun sequence.
ACCESSION   DASRZS010000265 DASRZS010000000
VERSION     DASRZS010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5150649"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="VFH83_06535"
     CDS             <1..1244
                     /locus_tag="VFH83_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET6486057.1"
                     /translation="RQRFFGVPFPVWYSKRAGEEGKIITVDVSELPVDPLVDTPKGYS
                     KDEVIADSDVMDTWATSSISPQINAKSLSCNKLFPADLRPQAHEIIRTWAFYTIAKAL
                     LHEKKIPWHHVLVSGWILDPDRKKMSKSKGNVVTPIEHLERFTADGVRYWAGCARLGT
                     DTAFEEKMLQVGKRLVTKIYNAGKFVLSQQGPRSRITSEIDRAFLAKLSACVQRTTEA
                     MEAYESARALSETETFFWTSFTDAYIELVKGRAKGSPPADAASQGSAVEGLRLALSVL
                     LRLFAPVLPYVTEEVWSWAFAIETRFQSIHLAPWPSAADFQAFSLPSDWNGFEAPAAA
                     LAAIHRHKTLSGMSVGSGLGMVVLAANARTVASLRPSVADLLGAARAAACELEERPDL
                     EDNQIEVVRAEPASDGGGPKG"
     gene            1241..3181
                     /locus_tag="VFH83_06540"
     CDS             1241..3181
                     /locus_tag="VFH83_06540"
                     /inference="COORDINATES: protein motif:HMM:NF027452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HET6486058.1"
                     /translation="MREAAPSIRVIGDQPAIALAAQELRRCLGRMTGRLPTVDPAETF
                     DPRQDALWIGLADAFPGEKLPKPNDPRLDDGIFIDTSGARGILCGPNPRSVLLAAYRF
                     LGELGARWVRPGMDGELLPRTDLDGRIVRVRETPSYRHRGVCIEGAVSLQHVADMIEW
                     LPRVGMNSYFIQFREGFTFFDRWYTHAGNPVAQPEEFTLDRARELVRQAARLIRERDL
                     IFHAVGHGWTCEPLGIPGLGWDYPAPPIPATAYPFLALVNGKRDLWQGVPLNTNLCYS
                     NPTVRRMIVEDILRYAQTHPEVDVLHAWLADGSNNQCECDGCKGTRPSDYYVRILNEL
                     DEQLTANGMRTRIVFLIYVDLLWPPETERLVHPDRFVLMFAPITRTYSSAFATAGPLP
                     PLAPFERNRLAFPRNVGENVAYLKAWQAQYQGDGFDFDYHLMWDHYADPGYSGVARVL
                     HEDIQRLEELGLNGLMSCQVQRAFVPTGLPMTVMGWTLWDKSRDLDAMAADYYAAAFG
                     VDGDRCRAYLEELTRLFDPPYLRGEKPLQNSLSAEGLESIPAVVQAFRTVIQANLERP
                     DLDPCRAASWRILERHGGLVELLSKALAARARGSQEEAARHGESVRALAWRLEPELHP
                     WLDTYLYTRVLSSRLEAKPPGT"
     gene            complement(3200..4561)
                     /locus_tag="VFH83_06545"
     CDS             complement(3200..4561)
                     /locus_tag="VFH83_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET6486059.1"
                     /translation="MNLIVIMLDSLRQDHVGAYGAGVVYAGVEPCRTPNIDRFAREAV
                     VFENAYPMGLPTIPARTELLTGQATLPFKPWEPLSAREITMAEILSQEGYTCGLVTDN
                     YHYRAPGMNFHRGFHAYEHIRGQEYDPWTSSSSRRSLDGYVNARYTEQFRGLVARFLA
                     NTDDFAREEDWFAAKVVESSCEWLRRNRDQRDVFLWLDSFDPHEPWDPPRRFDVYTDP
                     AYGGPRLILPMGGDAADWASPEEIRHIRGLYAGEVASVDHWLGRLFSCLQELGYYEDS
                     AIVLLADHGHPLGDHGKFLKGADRMHGELLHVPFMVRLPGGRGARRTGALVQFPDLLP
                     TVLELIGYGNCDAALHGRSFAPVLRGTADVHRDAVISGYHGGRDRCIRDLRWSWVERP
                     DGPDELYDLEADPRETQNLVDRHPQEARRLAARFGNLFRDAPRREVKGLQGRYELASG
                     VVP"
     gene            complement(4596..>4778)
                     /locus_tag="VFH83_06550"
     CDS             complement(4596..>4778)
                     /locus_tag="VFH83_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HET6486060.1"
                     /translation="VYDALRSKRSYKPAFDHETSVRIITEGDGRTMPDHFDPRILSVF
                     REMAEEFAKVYDRLST"
BASE COUNT          826 a         1557 c         1557 g          838 t
ORIGIN      
        1 cccgacagcg atttttcggt gttcccttcc ccgtttggta ttccaagcgc gcgggcgagg
       61 aaggaaaaat tattacagtg gatgtaagtg agcttccggt tgatccgcta gtcgataccc
      121 ctaaggggta tagtaaagat gaggtgattg ctgacagcga tgttatggac acgtgggcga
      181 cgtcgtcgat ttcgccgcag attaatgcca aatcgcttag ctgtaataag ttattcccgg
      241 ccgatttgcg cccgcaggcg catgaaatta ttcgcacctg ggcgttttac accatcgcca
      301 aagcgttgct ccacgaaaag aagattccct ggcaccatgt gctggtgtcc gggtggatcc
      361 tggatcccga tcgcaagaag atgtccaaga gcaagggaaa cgtagtcacc ccgattgagc
      421 acctggagcg gttcaccgcc gacggggtgc gctactgggc cggctgcgca cgcctgggaa
      481 cggatacggc gttcgaggag aagatgctcc aggtcggcaa gcgcctggta accaagatct
      541 acaacgcagg caagttcgtg ctctcccagc agggaccccg cagccgaatc accagcgaga
      601 tcgatcgcgc ctttctggcc aagctctccg cctgcgtcca gaggaccacg gaggctatgg
      661 aagcgtacga gtccgccagg gccctctccg agaccgagac tttcttctgg acgtcgttca
      721 ccgacgcgta catcgagctc gtcaaaggcc gggccaaggg atcgcctccc gccgacgccg
      781 cgtcccaggg gtccgcggtg gaagggctcc ggctggccct gagcgtcctg ctgcggctgt
      841 tcgctccggt ccttccgtat gtcaccgagg aggtctggtc ctgggccttc gccatcgaga
      901 cccggttcca gagcatccac ctggcgccat ggccgagcgc ggctgatttc caggcgttct
      961 ccctcccgtc ggactggaat gggttcgagg cgccggccgc tgctctggct gccattcatc
     1021 gccacaagac tctgtccgga atgtccgtcg gctcggggct cggcatggtc gtcctcgccg
     1081 ccaacgcgag gaccgtcgca tccctccgtc cctccgtagc cgacctcctc ggggcggccc
     1141 gggccgccgc ctgcgagctg gaggagcgac cggatctgga ggacaaccag atcgaggtag
     1201 tccgcgccga gccggcgtcc gatgggggag gcccgaaagg atgagggagg cggctccatc
     1261 catccgcgtc atcggggacc agccggcgat tgcgctggcg gcccaggagc tccgtcggtg
     1321 tcttggccgg atgacggggc gcttgccgac cgtggatccc gccgagacgt tcgatccccg
     1381 acaggacgcg ctgtggatcg gactggcgga cgcctttccc ggtgagaagc tcccgaagcc
     1441 aaacgacccg aggctggatg acggcatctt catcgacaca agcggagcgc gcggcatcct
     1501 gtgcggcccc aatccgcgca gcgtgcttct ggccgcgtat cgattcctcg gggagctcgg
     1561 ggcgcgttgg gtgaggccgg gaatggacgg cgagctcttg ccccggaccg atctggacgg
     1621 ccgcatcgtc cgcgtgcggg agacgccatc ctatcggcat cgcggggtgt gcatcgaggg
     1681 ggcggtttct ctccagcatg tggcagacat gatcgagtgg cttccccggg tcgggatgaa
     1741 ctcctacttc atccagttcc gggaagggtt caccttcttc gaccgatggt acacgcatgc
     1801 ggggaacccg gttgcgcagc cggaggagtt cactctggac cgcgcccggg agctggtgcg
     1861 ccaggccgcc cggctcatcc gggagcggga cctcatcttc cacgcggtcg gccatggatg
     1921 gacctgcgag ccgctgggga ttccgggcct gggctgggac tatccggcac cgccgatccc
     1981 ggcgacggcg tatccattcc tggctctggt gaacggcaag cgggaccttt ggcaaggcgt
     2041 tcccctcaac acgaacctct gctactccaa tcccacggtt cgccggatga tcgtcgagga
     2101 catcctccga tacgcgcaga cccatcctga ggtggacgtg ctgcacgcgt ggctggctga
     2161 cgggtcgaac aaccaatgcg aatgcgacgg gtgcaaggga acccgaccat cggattacta
     2221 tgtccggatc ctgaacgagc tggacgagca gctcaccgcc aacgggatgc ggacccggat
     2281 cgtgttcctg atctatgtgg atctcctgtg gccgcccgag acggaacggc tcgtccatcc
     2341 ggatcgcttc gtcctgatgt tcgcgccgat tacccgaacc tactcttccg cgttcgccac
     2401 cgcgggaccg ctaccgccgc tcgctccctt cgagcggaac cgtcttgcgt tccccaggaa
     2461 cgtcggggag aacgtggctt atctcaaagc gtggcaggcg caataccagg gcgacggatt
     2521 cgatttcgac taccatctca tgtgggatca ctacgcggat cccggctact ccggcgtggc
     2581 ccgggtgctg cacgaggaca tccagcgcct ggaagagctc ggtctgaacg gtctgatgag
     2641 ctgccaggtc cagcgcgcat tcgtgcccac cggtctcccg atgaccgtca tgggctggac
     2701 gctgtgggac aagagccgtg acctcgacgc catggccgcg gattactacg ccgcggcctt
     2761 cggagtggac ggcgaccgct gtcgcgccta cctggaagag ctgacgcggc tcttcgatcc
     2821 gccgtatctc cgcggggaga agccgcttca gaacagcctg agcgcggaag gtctggagag
     2881 catcccagcc gtggttcagg cattccggac cgtgatccag gccaacctcg agcgcccgga
     2941 tctggacccc tgccgcgccg catcctggcg gatcctggag cgacacggcg ggctggtaga
     3001 gctgctgtcg aaggcccttg ccgcgagagc ccgcgggtcc caggaagagg cggctcgtca
     3061 cggggagtcc gtgcgcgccc ttgcgtggcg gctggagccc gagcttcacc cctggctcga
     3121 cacgtacctt tatacccggg tgctctcctc ccgcctggag gcaaaacccc ccggcacctg
     3181 accgccggtg gggcccgatt cagggtacga cgcccgaggc gagctcgtat cggccttgca
     3241 ggcctttgac ctcccgtcgc ggagcgtccc ggaacaggtt cccgaatcgc gccgccagcc
     3301 gtctcgcttc ctgcgggtgg cggtccacca ggttctgggt ctcccgggga tctgcctcca
     3361 ggtcgtacag ctcgtctggg ccgtccggtc tttccaccca agaccagcga aggtcacgga
     3421 tgcatcgatc acgaccgccg tggtagccgc tgatgaccgc gtcccggtga acgtccgccg
     3481 taccccggag gactggcgcg aagctacgtc cgtgaagggc cgcatcgcaa ttgccatagc
     3541 cgatgagctc cagaacggtg ggcaaaagat ccggaaactg gaccagcgcc ccagtccgtc
     3601 gggcgccgcg tccccctggc aaccggacca tgaacgggac gtgcaggagc tcgccgtgca
     3661 tccgatctgc gcccttgagg aacttcccgt gatctccgag ggggtggccg tggtccgcaa
     3721 ggagcacgat cgcggaatcc tcgtagtagc ccaactcctg gagacacgaa aacagccggc
     3781 ccagccagtg atccacgctg gccacctccc ccgcgtacag gccgcgaatg tgacggatct
     3841 cctcggggct cgcccaatcg gcggcatcgc cgcccatcgg aaggatcagc cgcgggccac
     3901 cgtaggcggg atcggtatac acgtcgaaac ggcgcggcgg atcccagggc tcatgggggt
     3961 cgaaggagtc cagccacaga aacacgtccc gctggtcccg gtttcgccgc agccactcgc
     4021 aggatgattc tacgaccttc gcggcaaacc agtcctcctc ccgggcgaag tcgtccgtgt
     4081 tcgccaggaa gcgggccacc agccctcgga actgctcggt gtaccgggca ttcacgtagc
     4141 cgtccaggga acgacgagac gacgaagagg tccacgggtc gtattcctgg ccgcggatgt
     4201 gctcgtaggc gtgaaagccg cgatggaagt tcatcccggg ggcgcggtag tggtagttgt
     4261 ccgttaccag tccgcaggta tacccctcct gcgagaggat ctcggccatg gtgatctctc
     4321 gagctgagag cggctcccag ggcttgaacg gcagagtcgc ctggccggtg agaagctcgg
     4381 tgcgagcggg gatggtgggg aggcccatgg ggtaggcgtt ctcgaacacc acggcctccc
     4441 gagcgaaccg gtcgatgttc ggcgtcctgc agggctccac tccggcgtag acgactcccg
     4501 ccccgtaggc gcctacgtgg tcctggcgaa ggctgtccag catgatcacg atgaggttca
     4561 tgggctctct ccgggcgccg cgccgcgccg accgctcagg tggagaggcg gtcgtagacc
     4621 ttcgcaaact cctccgccat ctcccgaaac acggacagga tccgaggatc gaagtgatcc
     4681 ggcatggttc ggccatcccc ctcggtgatg atccgaacgc tggtctcgtg gtcaaacgca
     4741 ggcttgtagc tgcgcttgga gcgcaaggcg tcgtagac
//
LOCUS       DASRZS010000266         6072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2841710, whole genome shotgun sequence.
ACCESSION   DASRZS010000266 DASRZS010000000
VERSION     DASRZS010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2841710"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VFH83_06555"
     CDS             <1..946
                     /locus_tag="VFH83_06555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6486061.1"
                     /translation="RQTVTPRVIDLNETARGMLKMLQRMIGEEITLELAAESPLWPVR
                     VDPSQIDQMLANLCVNARDAIHGVGAIRIETGNRSFDEAYCSTHPRLVAGDFVLLRVS
                     DDGDGMDQETLSHLFEPFFTTKAVGQGTGLGLATVYGAVRQNGGVIDVQSEPGHGSRF
                     SIYLPRYQGGAQAGSRTETGGRAEPASSPRDGHERILLVEDEPSLLRLTEQMLVRQGF
                     AVLAARTPSRCLELAAGASEIQLLLTDVVMPEMNGSELAKRVRSLHPGIRCLFMSGYT
                     ADTISDHGVLDEKLAFIHKPFSMEDLATKIRETLEGEP"
     gene            1083..1436
                     /locus_tag="VFH83_06560"
     CDS             1083..1436
                     /locus_tag="VFH83_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486062.1"
                     /translation="MHSRYLKILGVVSVFSMITALSLFAGPSDIVMDRSVTPTSDIQY
                     YYAVHGIVQTLKNMVSAELRNEFDLYGDPATISMTLGTTTFTLPASNLVALQITTPDS
                     TQNGANPASTNQNVG"
     gene            1656..3350
                     /locus_tag="VFH83_06565"
     CDS             1656..3350
                     /locus_tag="VFH83_06565"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6486063.1"
                     /translation="MSDHVRELEERLAETTDLRATIDLLNDLAWEVRDTDPVRSLSLS
                     ETALRRSTSAPFEAQPYREGWVKSLRGLAHSNRRAGNLSVSLSQSMDALAFLESAPIP
                     DVTVDILRNVTIILGSLGNYAEALESGLKALRLAQSAGDRIREAAVLGSMGVVYVHAK
                     HGEESLRTFRQVLQINRELGQTREEALTLNNQSLAFRAVGDNANALAASLEALRLAKE
                     SGFGALVVTATGTVGESYLASGEAPKACEYFDRYLTAARSAGSKRDEAWALMLLGETH
                     VRRSSVASARECLARALEITQEVGLRSEEARCRKLLADLHEKQGDLAEALAQFRLYHE
                     LSESIFSEQTASRIANLQVIRQVEMARRDAEIQYLKTIELQREIEERKKSQRALEELA
                     AIDPLTGAFNRRQFLLLAEQEIREAAPGRRPVTLIMVDVDHFKSINDRHGHSIGDRVL
                     TEIAHLIRESLRKGEAVCRMGGDEFAILLPRTSAAQGQLIAQRVLEKVVSCPFQGNGG
                     SFSVTASFGVAEVSAAVPGTLEMLVDRADKAVYAAKRSGRNRVSTCASPMAGFLLP"
     gene            3519..4871
                     /locus_tag="VFH83_06570"
     CDS             3519..4871
                     /locus_tag="VFH83_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET6486064.1"
                     /translation="MKRSRRGSLVLLAVAALAILALAGCQRAPTEVTLGLLNASGMAW
                     ANTVELMQKQAEAYSAETGNKIKVEETLVPYANLHEKMLTEFVGKTGAYDMVSVVGDW
                     MAEFIKGGFLQPFDDYLKKDAPEGYPAQFPPALMEMQTGPDKKIYGLPFHDGPIMFYW
                     RKDLIADSANQAAFKKRFGYDLAAPKTWQQYLDQAQFFTKKGQMWGTMVAAKQGSQQL
                     TYDFYILLFNFGGKIFDDSWHPVFNSDQGVQALQFYVDLIDKYKVVDPAVTTFDVPDK
                     QAPILEGRVAMDWDWSHISMLSAQPDKSKVVGKLDYGTMPVLKEGMTPYTFDDYWVWT
                     IPSSSKHKDAAYKFIKWQVSKKNDALTYSTGQGIGVRYDNYRDPDLLAKYPYMAGIDK
                     ALSVNYFTPPKIPEFAKVNDAIGLECSNAIAGKKSVKQALDDAFKAVDDVMKQGGYYK
                     "
     gene            4998..5894
                     /locus_tag="VFH83_06575"
     CDS             4998..5894
                     /locus_tag="VFH83_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6486065.1"
                     /translation="MEPNAPRLRQPLIPPRIRIPLIMMAPAVVIMVGVGIFPLLYSLR
                     LMFTKWTMSTEPAPVWNGLRTIGRLFHDARLANSLMVTVQFTVGAVLLEMVLGLLLAL
                     LLTRRTRFIQLARTILLIPMMMTPVVVGMFWRFILNGDFGIVKYVLSLVGITAPVWTG
                     SVQWAMPTVIAIDAWQWTPFVFLILLAGLESLPTDVMEAARVDGATAWSRFWLITFPM
                     LLPFVFISALLRFIDAFRAFDNVFILTGGGPADATNLASLYVYWHGLRFFDMAYASTL
                     SYVVVMIVLVASYVFIRLERND"
BASE COUNT         1108 a         1967 c         1909 g         1088 t
ORIGIN      
        1 ccgtcagacc gtaacgccgc gcgtgatcga cctcaacgag accgcgcggg gaatgctcaa
       61 gatgctgcag cgcatgatcg gagaggagat caccctggag ctggccgcag agtcccccct
      121 gtggccggta cgcgtggacc cctcccagat cgaccagatg ctggcgaacc tctgcgtgaa
      181 cgcccgggac gccatccacg gggtcggtgc gatccgcatc gagaccggca accgaagctt
      241 cgatgaggcc tactgctcca cgcatcctcg cctcgttgcg ggagacttcg tcctcctgcg
      301 ggtgagcgac gacggcgacg gcatggacca ggagacgctc tcgcatctgt tcgagccgtt
      361 cttcactacg aaggcggtgg gacagggcac ggggttgggg cttgccaccg tttacggggc
      421 cgtcagacag aacggcggcg tgatcgacgt ccagagcgaa ccgggccatg ggtctcgatt
      481 cagcatctac ctcccgcggt accagggagg cgcgcaggcc ggcagccgga cggaaacggg
      541 cggccgagcc gagccggcct catcccctcg ggacgggcac gagaggatcc tcctggtgga
      601 agacgagccg tccctgctgc gattgaccga gcagatgctg gtccgacagg gctttgcggt
      661 cctggcggct cggaccccga gccggtgcct ggagctggcc gccggggcga gtgagatcca
      721 gcttcttctg accgacgtgg tgatgcccga aatgaacggg agcgagctgg cgaagcgggt
      781 gcggtccctg caccccggca tccggtgcct cttcatgtcc ggctacaccg cggacacgat
      841 ctccgatcac ggcgtgctgg acgaaaagct ggccttcatc cacaagcctt tctccatgga
      901 ggatctcgcc acaaagatcc gcgagaccct ggagggcgag ccttaaccgt tcgttcatta
      961 tagagcgggg cattctcaca ggtttcacgt ccagtttatt ggatccggcg ctcgccgtcg
     1021 cgaacctaga cgtgcgggtg ttgcccgctg tttcatcgac ccttttccaa ggagcatctg
     1081 tgatgcactc tcgatatctc aagatcctcg gtgtggtctc tgtcttctcc atgataacgg
     1141 cgctctcgct cttcgccggc cccagcgata tcgtcatgga ccggtcggtc acccccacca
     1201 gcgacatcca gtactactat gccgttcacg ggatcgttca gaccctgaag aacatggtct
     1261 ctgccgagct gcgcaacgag ttcgacctct atggcgaccc ggcgaccatc agcatgacgc
     1321 tgggaacgac caccttcacg ctgccggcca gcaacctcgt ggcgctccag atcaccacgc
     1381 cggactccac gcagaacggc gccaaccccg cgtccaccaa ccagaacgta ggatagcctc
     1441 gaccgccggt cactcctctc ttgcttgccg caagcagagg agtggctgga tctcaagagc
     1501 gtcctcgatc ggcagtgctc cgagaccctg ggatgggacc ggatccagga acgagttgtg
     1561 gagagcgcgg ccgggcagta gaattcattc cgcgtgggcc cgcgtgccgg gcagactccg
     1621 ggagcggttt gcacctgcag aatccggaac gatcgatgag cgaccatgtc agagaactcg
     1681 aagagcgact ggcggagacc actgacctcc gggccacgat cgacctcctg aacgatctgg
     1741 catgggaggt gcgggacacg gatcccgtcc gttccctgag cttgagcgag acggcgctcc
     1801 gccggtccac ctccgcgccg ttcgaagctc agccgtatcg ggaaggatgg gtcaagagcc
     1861 ttcggggtct cgcgcacagc aatcggcggg ctgggaacct ttccgtgtcg ctctcccagt
     1921 cgatggatgc gctggccttc ctggagagtg ccccgattcc cgatgtgacc gtcgacatcc
     1981 tgagaaacgt gacgatcatt ctcgggtccc tcggaaacta cgccgaggcc ctcgagtccg
     2041 gcctgaaggc gctgcgcctt gcccagtccg cgggagaccg gatacgcgag gccgcggtcc
     2101 tggggtcgat gggcgtggtc tacgtccatg caaagcacgg agaagagagc cttcgcacct
     2161 tccgccaggt gctgcagatc aaccgagagc tgggtcagac ccgggaggag gctctcacgc
     2221 tgaataacca gagcctcgcc tttcgcgcgg tcggggacaa cgcgaacgcc ctcgccgcga
     2281 gcctcgaggc gcttcgtctc gcgaaggagt ccggcttcgg ggcccttgtg gtgaccgcga
     2341 ccggcaccgt tggcgagtcc taccttgcca gcggcgaggc cccaaaggcc tgcgaatact
     2401 tcgaccgata cctgaccgcc gcgcgatcgg cgggctccaa gcgcgacgag gcctgggcgc
     2461 tgatgctgct cggagagact cacgtccggc ggagctccgt tgcctccgcc cgcgaatgcc
     2521 tggcccgggc tctggagatc acccaggagg tcggtctgcg ctcggaggag gcccggtgcc
     2581 gcaagctgct ggccgacctg catgagaagc aaggagacct cgcggaggcg ttggctcagt
     2641 tcaggctgta ccacgagctc agcgagtcga tcttcagcga gcagaccgcg agccggattg
     2701 cgaacctcca ggtcatccgc caggtcgaga tggcgcgaag ggacgccgaa attcagtacc
     2761 tgaagacgat cgagctccaa cgcgagatcg aggaacgcaa gaagagccag cgggccctcg
     2821 aggagcttgc cgctatcgat cccctgaccg gggccttcaa ccggcgccag ttcctcctgc
     2881 tcgcggaaca ggagatccgc gaggcggctc ccgggcgaag gcccgtgacc ctgatcatgg
     2941 tggatgtgga ccacttcaag tcgatcaatg accggcacgg acactccatc ggcgatcgcg
     3001 tgctcaccga gattgcgcac ctgatccggg agagtctccg caagggggag gccgtctgcc
     3061 gcatgggcgg agacgagttt gcgatcctgc ttcccaggac ctctgccgca cagggtcagt
     3121 tgatcgccca gcgcgtgctg gagaaagtcg tctcctgtcc gttccagggc aacggcggct
     3181 ccttctcggt gacggcgagc ttcggagtgg cggaggtcag cgccgcggtc ccggggacac
     3241 tcgagatgtt ggtggatcgc gccgacaagg ccgtgtacgc ggccaagcgc tccgggagaa
     3301 accgcgtctc gacgtgcgcc tccccgatgg cgggcttcct cctgccctga gacgggtccc
     3361 cccaggacgt ctccccggtc tccccggact tcggcttgac aggtggttgg gccggcctga
     3421 tactccggtt gtctttgatc gcgtttcctg gtgggtcagg tttcggtcta ctccgttgga
     3481 gtagtcagct ttgtccgtac ctagaaggag gcacccgaat gaagaggagt aggagaggct
     3541 ccctggtcct gctcgccgtc gccgcgctgg ccatcctggc cctggccggc tgccagcggg
     3601 cccccacaga ggtaaccctc ggccttctga acgcttccgg gatggcgtgg gccaacaccg
     3661 tggagctgat gcagaagcag gcagaagcgt acagcgcgga aacgggcaac aagatcaagg
     3721 tcgaggaaac cctggtcccg tacgccaacc tgcacgagaa gatgctgacg gagttcgtcg
     3781 gcaagaccgg cgcctatgac atggtctccg tggtcggcga ctggatggcc gagttcatca
     3841 agggcggatt cctgcagcct ttcgacgact atctgaagaa ggatgcccct gagggctacc
     3901 ccgcgcagtt cccaccggcc ttgatggaga tgcagacagg cccggacaag aagatctacg
     3961 gcctgccctt ccacgatggc ccgatcatgt tctactggcg caaggacctg attgccgact
     4021 ccgccaacca ggccgctttc aagaagagat tcggctatga ccttgcggcc ccgaagacct
     4081 ggcagcagta cctggaccag gcgcagttct tcaccaagaa gggccagatg tggggcacga
     4141 tggtggccgc caagcaaggc agccagcagc ttacctacga cttctacatc ctgttgttca
     4201 acttcggcgg gaagatcttc gacgatagct ggcatcccgt attcaacagc gaccagggcg
     4261 tgcaggcact ccagttctac gtagacctga tcgacaagta caaggtggtg gaccccgcgg
     4321 tcacgacgtt cgacgtcccc gacaagcagg cccccatcct cgaaggccgc gtggcgatgg
     4381 actgggactg gagccacatc tccatgctgt cggcccagcc ggacaagtcc aaggtggtgg
     4441 gcaagctgga ctacggcacg atgccggtgc tcaaggaggg catgaccccg tacacgttcg
     4501 acgactactg ggtctggacc atcccttcca gcagcaagca caaggacgcc gcctacaagt
     4561 tcatcaagtg gcaggtgagc aagaagaacg acgcgcttac ctacagcacg gggcaaggta
     4621 tcggagtgcg gtatgacaac taccgcgacc ccgacctgct cgccaagtac ccgtacatgg
     4681 cgggaatcga caaggcgctg tcggtcaact acttcactcc gcccaagatc cccgagttcg
     4741 ccaaggtcaa tgacgccata gggctggaat gctccaatgc catcgccggc aagaagagcg
     4801 tgaagcaagc cctggatgac gcattcaagg ccgtcgacga cgtcatgaag cagggtggct
     4861 actacaagta ggacccgctt cgtcaaacga tgggcgcgcg agcgcgcgcc catcgggtcc
     4921 gtgcaggagc ggcgcaggac gaagccgcgc gggccggccc ccgtgtgcgc acggggcgcg
     4981 cgatggacga tacccggatg gaaccgaacg caccgcggct gaggcagccg ctgatcccgc
     5041 cccgcattcg catccccctg atcatgatgg cgcccgccgt ggtgatcatg gtgggcgttg
     5101 ggatcttccc gctgttgtat tccctgcgcc tgatgttcac gaagtggacg atgtccacgg
     5161 agcccgctcc cgtctggaat ggccttcgca ccatcggtcg gctgttccat gacgcgcggt
     5221 tggccaactc cctgatggtc accgtgcaat tcaccgtggg cgccgttctt ctggagatgg
     5281 tcctcggatt gctcctggcg ctgctcctca ccaggcgcac gcgcttcatc cagctcgcgc
     5341 gtaccatcct cctgatcccg atgatgatga cccccgtggt ggtggggatg ttctggcggt
     5401 tcattctcaa cggtgacttc gggatcgtga agtacgtgct ctctctcgtg ggtatcaccg
     5461 ctccggtgtg gaccggatcc gtgcaatggg ccatgccgac cgtcatcgcc atcgacgcgt
     5521 ggcagtggac cccgttcgtt ttcctgatcc tgctggcggg actggagagc ctccctaccg
     5581 acgtgatgga ggccgcgcgc gtggacggcg cgacggcttg gagccgcttc tggctcatca
     5641 ccttcccgat gctccttccg ttcgtcttca tctccgcgct cctgcgcttc atcgacgcct
     5701 tccgggcctt cgacaacgtc ttcatcctga cgggcggcgg tcccgccgac gccaccaacc
     5761 tcgccagcct ctacgtctat tggcacggac tgcggttctt cgacatggcg tacgcctcca
     5821 cgctgtccta cgtggtggtg atgatcgtcc tggtggcctc ctatgttttc atccggctgg
     5881 aaaggaacga ttgacgtgag tgcacgacac ctcggccggc tgcgcacgca gatcgggcac
     5941 ggcatccaga acgtcatcgt catcgtggtc atgattctga cccttgcccc gtttctctgg
     6001 gtggcctcca actcgatcaa gccgcgtccc ctgatctacg agtacccgcc gaagctgttg
     6061 ttcaagccga cc
//
LOCUS       DASRZS010000267         3150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_145119, whole genome shotgun sequence.
ACCESSION   DASRZS010000267 DASRZS010000000
VERSION     DASRZS010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145119"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VFH83_06580"
     CDS             <1..425
                     /locus_tag="VFH83_06580"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6486066.1"
                     /translation="YLFEDGSRFNHPVFLEKLQLYPRIRFAMAHLSWPWCDECLAVMG
                     RMQAASRDGGFPWQSFIDTTPGTPPYIRRQAVGNAIDFCGPERLLFGSDSMLPGDFTY
                     QRTVRTADLALYRELGLQPAQIERVMSGTADQLFPAKT"
     gene            431..2224
                     /locus_tag="VFH83_06585"
     CDS             431..2224
                     /locus_tag="VFH83_06585"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HET6486067.1"
                     /translation="MMRPRGKSPSPARKANPTDAFVRPWFEETVGKPAKRRRHPYPRE
                     LFREREVFPLLVLDGGAFFWLNDRMNGRADGRAILEGLASGKLLEPWSKDGVLDWELP
                     LRRHGGSTLRTPLEERVWVHRLYFLLPIAQEFFRTGDESWARTWIRYFRAWAERHPRS
                     ADEAALRDDFVWFDMQVCWRLLVLIHSVYLLSGSSKLTRADWGFVYAAIIEHANHALA
                     EATSEIARGAQGANHSLQRGAALLYAGVLFPELALATECVDAGRTILTWQMREQVLSD
                     GGSVEGSPSYSHFIARYYAEAWALLRKNRMGSIEGLETTIQRQYEWLFHAASPAGLSL
                     QIGDSYALDARADLKTASRLFPLSIAGARRSTIFRESQFAVLRNARFDLYVDSMKMRE
                     DHQHVGRPNLLLFADGEPLLVDSGCCNYDWMIQEHYLRGAWAHNVVLVPALQEPMLDV
                     YVHNAELRQRYSDIAITEFVTGDRGSRIAVTSTVRQARGSYTWERQVVLEPDRVRILD
                     RVLSEDEVDCRQIFHLARTNVALAEGGRVAVMRHAGKDASWEHVEPARFFTLSYQPAV
                     DERNALVISPTLICEARGNPCRFEVVLTLSR"
     gene            2270..2773
                     /locus_tag="VFH83_06590"
     CDS             2270..2773
                     /locus_tag="VFH83_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET6486068.1"
                     /translation="MDQKLSILFARRSIRKYTSAPIGAADVRSILEAGMAAPSANDRQ
                     PWQIVAVQDRTMLRELADAHPYGRMLADAPLGIAVCGDPSVSTWWEVDCAAATENMLV
                     AAAGLGLGGVWLGVHGDAERERSIRKVLGVPDRIGILSLIAIGHPAEHKEPRTQFDPS
                     KVHTDRW"
     gene            complement(2776..>3150)
                     /locus_tag="VFH83_06595"
     CDS             complement(2776..>3150)
                     /locus_tag="VFH83_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486069.1"
                     /translation="CPWGPRELDEALTRIFAQSYPMLAPSIDVFTPLIYARKSGRGPD
                     WGAGFLRDARQFVPADRKVLLILDALDAPDCLTATAASEIPSWGLQLYGGAEVFSDPA
                     KAKVFRKAVEAIRSATAGGSGA"
BASE COUNT          520 a         1061 c         1056 g          513 t
ORIGIN      
        1 tttacctctt cgaggacggc tcgcggttca accatcccgt tttcctcgag aagctccagc
       61 tctatccccg gatccggttc gccatggcgc acctctcctg gccgtggtgc gatgaatgcc
      121 ttgccgtgat gggacggatg caggcagcct cccgcgacgg cggctttcca tggcagagct
      181 tcatcgacac cacgccgggg acgccgccgt acatacggag acaggcggtt ggaaacgcga
      241 tcgacttctg cggtcccgag cggcttctct tcggctccga cagcatgctc cccggtgact
      301 ttacctatca gcggacggtg cggaccgcgg acctggcgct gtatcgggag ctcgggttgc
      361 agcccgcgca gatcgagcgc gtgatgtccg gtaccgccga ccagctcttt cccgcgaaga
      421 cctgagggcg atgatgcggc ccagaggcaa atcccccagc cccgcgcgga aggccaatcc
      481 gacggatgca ttcgtccggc cttggttcga agaaacggtc gggaaaccgg caaagcggcg
      541 aaggcacccc tacccccggg agctctttcg agagcgcgag gtattcccgc tcctggtcct
      601 ggatggcgga gccttcttct ggctcaacga ccggatgaac ggccgggccg atggaagggc
      661 aatcctggaa ggcctggcat ccgggaagct cctcgaacca tggtcgaagg acggagtgct
      721 cgactgggag ctgccgcttc gccgccacgg cggctccacc ctgaggacgc cgttggagga
      781 gcgggtctgg gtgcaccgac tctacttcct gttgccgata gcgcaggagt tctttcgtac
      841 cggagacgag tcgtgggcgc gcacctggat ccggtacttc cgagcctggg ccgaacgcca
      901 tccccggtcg gcggatgagg cggccctcag agacgacttt gtctggttcg acatgcaggt
      961 gtgctggcgc ctcctggttc tcatccacag cgtctatctc ctgagcggct cgtcgaagct
     1021 cacgcgggcg gactggggat tcgtttacgc cgccatcatc gagcacgcca accacgccct
     1081 tgccgaggcc acgagtgaga tcgcccgcgg cgcccagggc gcgaatcatt cgctgcagcg
     1141 cggggccgcc ctcctgtacg cgggggtgct cttcccggag ctcgctctcg ccaccgagtg
     1201 cgtggacgcc ggccggacca tcctgacctg gcagatgcgg gagcaggtcc tttccgacgg
     1261 cggaagcgtc gagggcagcc cgagctacag ccacttcatc gcccgctact acgcagaggc
     1321 atgggcgctc ttgcggaaga accgcatggg gtcgatcgag gggctggaga ccacgatcca
     1381 gcgccagtac gagtggttgt tccacgcggc cagccccgcg ggcctgagcc tccagatcgg
     1441 ggactcctac gcgctggatg cccgggcgga tctcaagacc gcgtcccgac tgttccctct
     1501 gagcatcgcg ggagcccgaa gatcgacgat cttccgcgag agccagtttg cggtcctgag
     1561 gaatgcccgg ttcgatctct acgtggactc catgaagatg cgcgaggacc accagcacgt
     1621 gggccggccc aacctgctgc tcttcgccga tggggagcca ctcctggtgg actccggctg
     1681 ctgcaactac gactggatga tccaggagca ctacctgcgc ggcgcctggg cgcacaacgt
     1741 ggtcctggtg ccggcgctcc aggagccgat gctcgatgtc tacgtgcaca acgccgagct
     1801 gagacagcgg tactccgaca tcgccatcac cgagttcgtg accggggacc gcgggtcccg
     1861 catcgccgtc accagcaccg tccgtcaggc ccgtggcagc tacacctggg aacgccaggt
     1921 cgtcctcgag cccgaccgcg tgcggatcct ggaccgtgtc ctctccgagg atgaagtgga
     1981 ctgccgccag atcttccacc tggcacggac caacgtggcg ctggcggagg gcggccgggt
     2041 ggcggtgatg cggcacgcgg gcaaggacgc ttcgtgggag cacgtggagc ccgcgcggtt
     2101 cttcacgctc agctaccagc ccgcggtgga cgaacgcaac gccctcgtca tctcccccac
     2161 gctgatctgc gaggcgcggg gcaacccgtg ccggttcgag gtggtcctga cgctgtcccg
     2221 ctgaggcacg gccctggtcc aagccgactc gacgtcgtag gatccgacca tggaccagaa
     2281 gctgagtatc ctctttgcgc gacggagcat ccgcaagtac acctcggcgc ccatcggcgc
     2341 cgccgatgtg cgcagcatcc tggaggcggg gatggccgcc ccgtcagcca acgatcgcca
     2401 gccgtggcag atcgtcgcgg tccaggatcg gacgatgctc cgcgagctgg cggatgccca
     2461 cccctatggg cggatgctgg cggatgcgcc cctgggcatc gcggtctgcg gtgacccatc
     2521 ggtctccacg tggtgggagg tcgactgcgc ggcggcgacg gagaacatgc tggtggcggc
     2581 ggccggactg ggactgggcg gcgtctggct gggtgtccac ggggacgccg agcgggagag
     2641 gtcgatccgc aaggtcctcg gagtccccga ccggatcggc atcctcagcc tgatcgccat
     2701 cggtcatccc gccgagcaca aggaaccgcg cacccagttc gatccgtcca aggtccatac
     2761 cgatcggtgg taggtctacg cgccggagcc cccggccgtg gcggaccgaa ttgcctccac
     2821 ggccttacgg aagaccttgg ccttcgcggg gtccgagaag acctcggcac caccgtagag
     2881 ctgaagcccc cagctcggga tctctgaggc ggccgtggcc gtgagacagt ccggagcatc
     2941 cagggcatcc aggatgagca ggaccttccg atccgctgga acgaactggc gggcatccct
     3001 caggaagccc gcgccccaat ccgggccgcg ccccgacttc cgcgcataga tgagcggcgt
     3061 gaagacgtcg atggagggag cgagcatcgg gtagctctgg gcgaagatcc gcgtgagcgc
     3121 ctcgtcgagc tccctcgggc cccacgggca
//
LOCUS       DASRZS010000268         5891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4768234, whole genome shotgun sequence.
ACCESSION   DASRZS010000268 DASRZS010000000
VERSION     DASRZS010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4768234"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VFH83_06600"
     CDS             complement(<1..476)
                     /locus_tag="VFH83_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6486070.1"
                     /translation="MVRRWILVLACAAALSAPLAAQTRVLRVLTHSSFAASQSVLDAF
                     QQANDVKLQFISGGDAGEALNKAILARGNPLADVFYGVDNTFLSRALEADILQPYDSP
                     SLAQIPAGLRLDASNRLLPVDFGYVDLNYDPKYFADKGLTTPQTLEDLTLPAYRGLL"
     regulatory      complement(581..697)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(724..1593)
                     /locus_tag="VFH83_06605"
     CDS             complement(724..1593)
                     /locus_tag="VFH83_06605"
                     /inference="COORDINATES: protein motif:HMM:NF042560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6675 family protein"
                     /protein_id="HET6486071.1"
                     /translation="MAGRAAAGALAIAFPIRYRQDSMRLPLAAIRIGRSRPLAGLVLV
                     VLLLPAPPCGSDPLTRFVEPASLSALRSGDTLVSRLAADGAPRLLPRFGAPEGIAPDH
                     LTLGVEMLRLLVGNGEPFESPQGMLRLYNSLHAVSTLKGIPYYSVTRGTTQVLFSESF
                     AVAAPQGNLRIPDPVFDSIPLTDVLYTFQADQSFGRNTYREVFSAAEDHLSVRIENLT
                     NIMVLFVPIIRPHDLVSYVALMPAGNELAFYGLAYLTTGFPIGDRHSREESLLNRLKA
                     MEAWLAGRVGATP"
     gene            1547..2653
                     /gene="murG"
                     /locus_tag="VFH83_06610"
     CDS             1547..2653
                     /gene="murG"
                     /locus_tag="VFH83_06610"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET6486072.1"
                     /translation="MGNAIASAPAAARPAMIAIAGGGTAGHVFPGIAVAEELGRNVVW
                     IGSSGGVERGLVTEAGIPFHGIPAGKLRRYLSLRNLTDVLRVAAGVFASLRILRKERP
                     RLLFSKGGYVSVPPVVAARLFRIPAFTHESDFDPGLATRINLRLCERVLVSYPQTLEF
                     LPPRYREKAIVTGNPVRAAFYAADPRKGRSFVGCDAETPLLLVLGGSLGSSSVNRLIE
                     AARPRLTERWFVVHQMGAREARPAREARYHAAAFFQAELPDLMAAARIVVCRAGANTL
                     AELAALGRPSVLIPLPASGSRGDQIRNAELFRSRGAASVLREEEATADALVSLLDSLL
                     AEPGRLVSMGTQAKGLSAGVPAREIARLIRARIS"
     gene            2653..3972
                     /locus_tag="VFH83_06615"
     CDS             2653..3972
                     /locus_tag="VFH83_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486073.1"
                     /translation="MFENIIGQTETLHGLSEEIAAGILPRAILFAGPAYSGKLSTALE
                     VARILTCIGDNGAWTCECSSCRQQKELSHPQTVLLGSRYSEVEIAASAGSLLASRRPV
                     TQYLFLRAVRKLTRRFDPSIFDAEDTRVRATREKAAAIEELLSAVEPGRELGSERELT
                     AQVDEILGACLQLLALQRADGVSIAQVRRLASWAHLGAGGQQKVAIIENADRLQDSAR
                     NALLKLLEEPPQSVHFLLLSTRRAAIAPTILSRLRPYAFRQRDAAEEREVLARVFHVE
                     DAPYDSLRAFFMAWRALSPERVSILAKRFLELVDAEGNGGRADILNELAELTADSSGK
                     RERGARRTAASQREAALAFLEELTVQLSARLRAAPDAPESAEQGSEAIREAVTRIDAL
                     NISPPLVLESLFYRMAEDARSLTGKPAAEAGSADAAAGAPTIRSGDP"
     gene            3969..5135
                     /locus_tag="VFH83_06620"
     CDS             3969..5135
                     /locus_tag="VFH83_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6486074.1"
                     /translation="MRRFIQRSLDKLPKMDVDQIRGLIVSLAQENDLLVMLLESMTDG
                     IIVTDREHRVSFYNKSAERLFPFDRDEVRERVLWECIADRELSAFFAVNLRNQEKVVD
                     REFTFTEGNLRTLSVSLLPLVRDGSVQGNVVHIEDVTEKRSKEARLRRAESLASLTTL
                     AAGVAHEIKNPLGSMGIHLQLIRKKMSGKKCIEAKDIDQHLSVMGEEVDRLNRIVVDF
                     LFAVKPMDTRLEDGDINVVIQELLEFVRPEMDQAGVTLQADLAVGLPTLRLDPRYIKQ
                     ALLNLIKNAVAAMPGGGTLRIATERVGEEVRLRVSDTGTGIPDDIMDKIFEPYFTTKP
                     FGTGLGLTIVFKIVKEHFGDITVTSRASEGTTFTISLPVPQTEKILIDYKGEAR"
     gene            5132..>5891
                     /locus_tag="VFH83_06625"
     CDS             5132..>5891
                     /locus_tag="VFH83_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6486075.1"
                     /translation="MKLTILVADDEKNIREGLKEALMLDGYDVLLAADGKEALAAVNR
                     GEVDLIITDLKMPRLSGEELLRAVAAEYPTVPVIILTGHGTIESAVQAMHDGAYDFLT
                     KPVNLDRLSLLVKRALSTRELALQNRAMQEELERRSGFASIIGKSAEMRQVFEMVRQV
                     APSRSSVLITGESGSGKEMIAEALHYNSPRKDKPFIKVHCAALTETLLESELFGHEKG
                     AFTGAVARRRGRFELAHLGTLFLDEIGEVNQNVQI"
BASE COUNT         1026 a         1900 c         2007 g          958 t
ORIGIN      
        1 aggagcccgc ggtaggccgg cagcgtgagg tcttcaaggg tttgcggcgt cgtgagaccc
       61 ttgtccgcga agtacttcgg gtcgtagttg agatcgacgt agccgaagtc caccggcagg
      121 aggcggttgg atgcgtcgag acgcaggcct gccggtatct gggccaggga cggggagtca
      181 tagggctgca ggatgtcggc ttccagcgct ctgctcagga acgtgttgtc cacgccatag
      241 aacacgtctg ccagaggatt tccccgcgcc aggatggcct tgttgagggc ctctcccgcg
      301 tcgcctccgg atatgaactg gagcttgacg tcgttggcct gctggaaagc atccaggacc
      361 gactgggacg ccgcgaacga tgagtgcgtg agaacccgca ggacccgcgt ctgcgcggcc
      421 agcggcgctg acaacgcggc cgcgcaggcc aagaccagga tccatcttct gaccataaag
      481 cccccctcaa gcggcgcata tagattcccg gcacagccgc cagtgacggg attcgggcca
      541 cctactgccg tggcctggct acggggtacc gtagctctgt tgtggggagc actgtcggtt
      601 tccctgcgct ggtattaccc agatcaagtt caacgggtat gttctcaggc tcccagccgg
      661 ccctccgccg taccgggaac caccccacga ccagtaccaa tgtattgccg cagacgcctg
      721 cggtcaagga gtcgcaccga ccctgccggc cagccacgcc tccatcgcct tcaggcgatt
      781 cagcagagac tcttcccggc tgtggcggtc cccgatgggg aacccggtgg tcaggtaggc
      841 gaggccgtag aacgcaagct cgtttccggc cggcatcagg gccacgtagc tcaccaggtc
      901 gtgcggccgg atgatcggga cgaacaggac catgatgttc gtgaggttct cgatgcggac
      961 cgagaggtga tcttcggcgg ccgagaacac ttcccgatag gtgttgcggc cgaagctctg
     1021 gtcagcctgg aatgtataga ggacatcggt gagcgggatg gagtcgaaga ccgggtccgg
     1081 gatccgaagg ttcccctggg gagctgctac ggcgaaagat tcggagaaca ggacctgggt
     1141 ggtgccgcgc gttacggagt agtaggggat gcccttgagg gtgctcaccg catgaaggct
     1201 gttgtacagg cggagcattc cctgcggcga ctcgaacggc tctccgttgc caaccagcag
     1261 gcgaagcatt tccactccaa gggtgagatg gtccggcgcg atgccttcag gggcgccaaa
     1321 cctgggaagc agacgaggag ccccatcggc cgcaaggcgt gagacgaggg tgtcgccgga
     1381 tcggagggcg gagaggctgg cgggctccac gaatcgggtc agggggtcgg acccgcaagg
     1441 aggggcggga agcagaagca caaccaacac gagtcccgcc aacggcctcg aacgcccgat
     1501 gcggattgct gctaacggga gtctcatgct atcctgacgg taccgtatgg gaaacgctat
     1561 tgcaagcgca cccgccgccg cgcggcccgc catgatcgcg attgccgggg gcggaacggc
     1621 cggccacgtc tttccgggga tcgccgtagc ggaggagctt ggccggaacg tggtctggat
     1681 cggctccagc ggcggcgtgg aacggggtct cgtcacggaa gctgggatcc ccttccacgg
     1741 gattcccgcg ggcaagctcc gccgctatct ctcgcttcgc aacctcaccg acgtcctgcg
     1801 cgtggccgcg ggagtctttg cgtcccttcg gatcctgagg aaggagcggc cgcggctcct
     1861 gttctccaaa ggcgggtacg tgagcgtacc ccccgtggta gccgcccgcc tgtttcggat
     1921 tccggcgttc acccatgagt ccgacttcga tccgggcctg gccacccgga tcaaccttcg
     1981 cctctgcgag cgtgtgctgg tttcctatcc ccagacgctg gagttcctgc cgccgcgcta
     2041 tcgggagaag gcgatcgtca ccgggaaccc cgtgcgcgcc gcgttctatg cggccgaccc
     2101 gcgcaagggc cggagcttcg tcgggtgtga cgctgagacg cccctccttc tggtcctggg
     2161 tggcagcctg ggatcgagct ccgtgaatcg cctcattgaa gccgcaaggc ctcgactgac
     2221 cgagcgatgg ttcgtggtcc accagatggg agcgcgagag gcgcggcccg cccgagaggc
     2281 gcgctatcac gcggcggcgt tcttccaggc ggagctgccg gacctcatgg ccgccgcccg
     2341 gatcgtggtg tgccgggcgg gcgccaacac gctggccgag cttgccgccc ttggccgtcc
     2401 gtccgtcctc ataccgcttc cggcgagcgg gagccgggga gaccagattc gaaacgcgga
     2461 gctcttccgt tcccggggcg cggcaagcgt gctgagggaa gaggaggcca ccgcggacgc
     2521 cctcgtctcg ctgctggact ccctgctggc cgaacccgga cgtctggtgt ccatgggcac
     2581 gcaggccaag gggctcagcg ccggggtccc ggcccgagag atcgcacggc tcatccgcgc
     2641 ccgcatatcc tgatgttcga gaacatcatc ggccagacgg aaaccctcca cggcttgtcg
     2701 gaagagatcg ccgccggcat cctcccgcgc gccatcctgt tcgccggtcc cgcctacagc
     2761 gggaagctgt ccacggcgct cgaggtggcg cgaatcctga cctgcattgg ggacaacggg
     2821 gcctggacgt gtgagtgctc gtcgtgccgg cagcagaagg aactgtcgca cccgcagacg
     2881 gtgctcctgg gctcgcgcta ctccgaggtg gagatcgccg ccagcgccgg ctccctcctg
     2941 gccagccgaa ggccggtcac gcagtacctg ttcctccgcg ccgtccgcaa gctgacccgc
     3001 cgcttcgatc cgtcgatctt cgatgccgag gacacgcgcg ttcgcgcaac cagggagaaa
     3061 gcggcggcca tcgaggagct gctctccgcc gtcgagccgg gccgcgagct cggctcggag
     3121 cgcgagctca ccgcccaggt ggacgagatc ctgggagcct gtctgcagct tctggcgctc
     3181 cagcgcgccg atggcgtcag catcgcccag gtccgtcgac tcgcctcgtg ggcgcacctg
     3241 ggagccggcg gccagcagaa ggtggcgatc atcgagaacg cggaccgtct gcaggactcc
     3301 gcccggaacg cgctgctcaa gctcctggag gagccgcccc agagcgtgca cttcctactc
     3361 ctgtccactc ggcgggcagc catcgccccg accatccttt cgagactccg accctacgct
     3421 ttccgtcagc gggatgccgc cgaagagcgg gaggtgctgg cgcgcgtgtt ccacgtagag
     3481 gacgcacctt acgacagcct gcgagcgttt ttcatggcct ggagggcgct cagcccggag
     3541 cgcgtctcta tcctggcgaa acgcttcctg gagctggtgg atgcggaagg caacggcggc
     3601 agggcggaca tcctgaacga gctggccgaa ctgaccgcag actccagtgg aaagcgcgag
     3661 cggggggcgc ggcggaccgc ggcctcccag cgcgaggccg ctctggcgtt cctggaggag
     3721 ctgaccgtac agctctccgc gcgcctgcgg gccgcgcccg acgcgccgga gagcgccgag
     3781 cagggctccg aagctatccg cgaggcggtg acccgcatcg atgcgctcaa catcagcccg
     3841 ccgctggtgc tggagtctct cttctaccgc atggcggagg atgcgcggtc cctgacggga
     3901 aagcccgcgg cggaggccgg ctcggcggac gcggcggcgg gcgctccgac gatcaggagc
     3961 ggagatccat gagaaggttc atccagcggt ctctggacaa gctcccgaag atggacgtcg
     4021 accagatccg tgggctgatc gtttccctgg cgcaagagaa cgatctgctg gtcatgctgc
     4081 tggagtccat gacggacggg atcatcgtca cggaccggga gcatcgggtg agcttctaca
     4141 acaagtccgc ggagcggctg ttccccttcg atcgggacga ggtgcgggag cgcgtgctgt
     4201 gggagtgcat tgcggaccgc gagctctccg cgtttttcgc cgtgaacctt cggaaccagg
     4261 aaaaggtcgt agaccgcgag ttcacgttca ccgagggcaa cctgaggacg ctgtccgtta
     4321 gcctgctgcc gctcgtgcgg gatggaagcg tccagggcaa cgtggtacac atagaggatg
     4381 tgacggagaa gcgctccaag gaggcgaggc tgcgccgggc ggagagtctg gcctctctca
     4441 ccaccctcgc cgccggggtc gcgcacgaga tcaagaaccc tctgggctcc atggggatcc
     4501 acctgcagct catccgcaag aagatgtccg gcaagaagtg catcgaggca aaggacatcg
     4561 accagcacct gagcgtcatg ggggaagagg tggaccggct gaaccgcatc gtcgtggact
     4621 ttctcttcgc ggtgaagccc atggacaccc ggctggagga cggggacatc aacgtcgtca
     4681 tccaggagct cctggagttc gtccgtcccg agatggacca ggccggcgtg accctccagg
     4741 ccgaccttgc cgtcgggctt ccgacgcttc ggctggaccc acgctacatc aagcaggccc
     4801 tcctcaacct gatcaagaac gcggtagccg cgatgcccgg gggcggcact cttcgcatcg
     4861 ccacggaacg ggttggggag gaggtgcgcc tgcgggtttc ggatacgggc accggaatcc
     4921 ccgacgacat catggacaag atcttcgagc cgtacttcac caccaagcct ttcggtaccg
     4981 ggctggggct caccatcgtg ttcaagatcg tcaaggagca cttcggcgac atcacggtca
     5041 cctctcgtgc ctccgagggc accaccttca cgatctctct ccccgttccc cagacggaga
     5101 agatcctcat cgactacaag ggggaggcgc gatgaagctg accatcctgg tggccgacga
     5161 cgaaaagaac atccgcgagg gcctcaagga ggccctgatg ctggacgggt acgacgtgct
     5221 tcttgcggcc gacgggaagg aggcgctggc cgcggtgaac cgcggagagg tggacctcat
     5281 catcacggac ctcaagatgc ctcggctctc cggcgaggag ctcctgcggg cggtggcggc
     5341 cgagtatcca acggtgccgg tgatcatcct gaccgggcac ggcaccatcg agtccgcggt
     5401 tcaggcgatg cacgacgggg cgtatgactt cctcaccaag cccgtgaacc tggatcggct
     5461 gtcgcttctg gtgaagcggg cgctctccac ccgggagctg gcccttcaga accgcgccat
     5521 gcaggaggag ctggagcgcc gcagcggctt tgccagcatc atcggcaaga gcgccgagat
     5581 gcgccaggtg ttcgagatgg tccgccaggt ggctccttcc cgcagctcgg tcctcatcac
     5641 gggggagagc ggctccggca aggagatgat cgccgaggcc ctgcattaca actccccgcg
     5701 caaggacaag ccgttcatca aggtgcactg cgccgccctc acggagacgc tcctggagag
     5761 cgagctcttc ggtcacgaga agggcgcctt caccggggcg gtggcccgga ggcggggcag
     5821 gttcgagctc gcgcacctgg gcacgctctt cctggacgag atcggcgagg tgaaccagaa
     5881 cgtccagatc a
//
LOCUS       DASRZS010000269         5403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1687614, whole genome shotgun sequence.
ACCESSION   DASRZS010000269 DASRZS010000000
VERSION     DASRZS010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687614"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFH83_06630"
     CDS             complement(<1..416)
                     /locus_tag="VFH83_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486076.1"
                     /translation="MKPLLTAAVLCLASAGVMAQNVPVAPSGVVEYQGRTQVVVTIDN
                     LDLLAEGLGRLEPLDGHTGFGYRSVTVGGYYRVIPNLKLGAFYRLQAGARHDDDWIQT
                     GPNPDDWGWQDTTSRWENVLMLDASPRFLLSFLPGA"
     gene            complement(432..1106)
                     /locus_tag="VFH83_06635"
     CDS             complement(432..1106)
                     /locus_tag="VFH83_06635"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HET6486077.1"
                     /translation="MRPRHLRTVALALALLGCAPCLSWAISGRIVYLEGDVAVLSGGD
                     SRSAQIGDAIQQGDTVSTAALSLAVLDLANGTTVKLREKTTLAIDTLGDSAAVTLKAG
                     GVFTSIAHHLVGTFDVKASNAIAGVRGTQFFVAYGRTIDAVPDLWLCVQEGSVQVDLP
                     TAGQTVVVPAGRGITIVGGARLTVPRRYPWTRKLNWNVDPAAGSVVDRTSLDDAYLDL
                     LNQDYD"
     gene            complement(1188..2276)
                     /locus_tag="VFH83_06640"
     CDS             complement(1188..2276)
                     /locus_tag="VFH83_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6486078.1"
                     /translation="MNGKNIAREISRLMRDGPDRYYPGTPLVDWFQRAGDADLWRINS
                     HALANTLGVDRARLLATFIRLVKDGVVDLNWDFHCTDCNAVAGTHRHLNEATAGNHCP
                     LCNLDFRNTLDRNVEVTFTPTVRLWSVDKRFLAEQTRRTVELHGKKAIQLPPLFVSGM
                     DCLHVPLFREIFETETLSLRESLSVGQVCIMFTDIKGSTALYEKRGDSAAYALIRDHF
                     DILFRRVEQNDGVIVKTIGDSVMASFKRPVDGVAAAIAIQKAFTAFNQRENIKDEILV
                     KIGLHAGSTIMVNLNNRLDYFGQTVNIAARIQGTADGGDIIISSDIRKDRASIERMRG
                     HVRGLARRVEKLKGIAEAQEVFALRFAS"
     gene            2408..2941
                     /locus_tag="VFH83_06645"
     CDS             2408..2941
                     /locus_tag="VFH83_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HET6486079.1"
                     /translation="MQRQSIPFDRLRLPVVSLWDRQWLLLATGDFEAGEYNCMTVGWG
                     SFGVMWGRPHAVVVVRPTRFTWRLMERSDSFTLTAFAEEHRRKLSYCGSHSGRDGDKA
                     KACGLTACAAGHVRAPAFEEAELIVECRKIYQHDFDPARFLDPTIETNYPKKDYHRVY
                     YGQILAVSGIAAYRMEE"
     gene            2946..4079
                     /locus_tag="VFH83_06650"
     CDS             2946..4079
                     /locus_tag="VFH83_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6486080.1"
                     /translation="MDMTLPLKHPKPDSSYFLDVVMGRRSGPSPLVEYLVDDVVMKPI
                     VEGLLGREWAGEGSVGTAHEVRRGVVDRAAQTRYLDTYIDFWLRMGYDFVRFERDVGF
                     PERHLETSDTAPGSAKRRAWSDQHTGSIMSWKDYEKYPWPRLESFDFFPFEYINSHLP
                     DGMGMITCHGGGLYEHLSWIMSYEGLCLALYDAPDLVKAVAERVGSLLVGFYRHLLDL
                     DRVIAIFQGDDMGFKTGTLLAPEALRTYCLPWQRRLAAMSHERGRPYFLHSCGNLASI
                     MEDLIEDVRIDGKHSYENTIMPVTEFQQKYGTRIAVLGGLDINILTRSTPQEIRKQTA
                     NLIEVCGAHGRYAVGSGNSVPSYIPVENYLAMVDEAVKRRGLS"
     gene            complement(4116..5021)
                     /locus_tag="VFH83_06655"
     CDS             complement(4116..5021)
                     /locus_tag="VFH83_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HET6486081.1"
                     /translation="MPLPAPRSQLGRSESLLLCLTAYLISAMAAVAAGWAVGRFLGWH
                     SAVWMAAAGDLAATVVVFAFSAALDNSSVYDPYWSVAPVFIAGFWLASYGPARPRALL
                     ALACVALWALRLTANWLRRWRGLSDEDWRYADYRRLGAGYWPVSFLGFHLFPTIIVFL
                     GCLPLTAALAWNARPVGALDAVAVAVTLAAILIESTADRQLRAFLDSPREPGSTLETG
                     LWALTRHPNYFGEVLFWWGLYLFGLAADPSRWWTIVGPLGITALFLGVSVPMMDRRMT
                     AGHPGYAEQMRLRSAFLPLPPRREG"
     gene            complement(5039..>5403)
                     /locus_tag="VFH83_06660"
     CDS             complement(5039..>5403)
                     /locus_tag="VFH83_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HET6486082.1"
                     /translation="QARVFVMDQTALSAIGNAYADEILFAAGIHPKTSCASLSAEERR
                     KLYDSIRSVMAWGIEEVRKADRPIEEKVRDHMRVRNRKDQPCPVCGGTIRRAGVLGYD
                     SFFCPTCQPAVRKQAIPW"
BASE COUNT          954 a         1764 c         1797 g          888 t
ORIGIN      
        1 ttggcccccg gcaggaagct caggaggaac cgcgggctgg catccagcat cagcacgttc
       61 tcccaccggg aggtggtgtc ctgccagccc cagtcatccg gattgggacc cgtctggatc
      121 cagtcgtcat cgtggcgcgc cccggcctgg agacggtaga aggcgcctag cttgaggttg
      181 gggatgaccc gatagtagcc gccgacggtc accgagcggt agccgaagcc cgtatgaccg
      241 tccagcggct cgaggcggcc cagcccctcc gccagcaggt ccaggttgtc gatggtgaca
      301 accacctggg tccttccctg gtattccacc acgccggagg gcgccaccgg aacattctgc
      361 gccatcactc cggcggaggc caggcaaagg acggctgccg tcagcaaggg cttcatgtcc
      421 ccgcctttct cctaatcgta atcctggttc agaagatcga ggtaggcgtc atccaggctc
      481 gtccggtcca cgacgctgcc ggccgccgga tccacgttcc agttcagctt tcgagtccaa
      541 gggtagcggc gcggaaccgt gagccgggct cccccgacga tggtgatccc gcggcctgcc
      601 ggcaccacca ccgtctgtcc cgcggtgggc aggtccacct ggacggatcc ctcctgcaca
      661 cacaaccaga ggtccggcac ggcgtcgatg gtacggccgt aggccacgaa gaactgcgtg
      721 cctctcacgc cggcgatggc gttcgatgcc ttcacatcga aggtccccac caggtgatgg
      781 gcgatggagg tgaagactcc gcccgccttc aacgtcacgg cggccgagtc tccgagcgtg
      841 tcgatggcga gggttgtctt ttcgcgcagc ttcaccgtgg ttccgttggc gagatccaga
      901 acggccaggg agagcgccgc ggtggatacc gtgtccccct gctggatggc atcaccgatc
      961 tgagcgctgc ggctatcacc tccgctcagg accgcgacgt ccccttccag gtaaacgatc
     1021 ctgccgctga tagcccagga caggcatggc gcacacccca gaagggcaag cgccagagca
     1081 acggtccgga ggtgcctcgg ccgcatcgga tcctccccgc cgagaatcta cggctccggg
     1141 gagccggcgg caaccgtgga aggtgcggcc ctggctggtc ggccccctca ggaggcgaac
     1201 cgaagcgcga acacctcctg cgcctcggcg atccccttga gcttctccac gcggcgggcg
     1261 agtccgcgca cgtgtccccg catccgctcg atggaggcgc ggtccttgcg gatgtcggaa
     1321 gagatgatga tgtcgccgcc gtccgccgtt ccctggatcc gcgccgcgat gttgacggtc
     1381 tgcccgaagt agtcgaggcg gttgttgagg ttcaccatga tggtgcttcc ggcgtggaga
     1441 ccgatcttga ccaggatctc gtccttgatg ttctcccgct ggttgaaggc ggtgaacgcc
     1501 ttctggatgg cgatggcggc cgctacgccg tccacgggac gcttgaagga ggccatgacg
     1561 gagtccccga tggtcttgac gatgacccca tcgttctgct ccacgcgtcg aaagaggatg
     1621 tcgaagtggt cgcggatcag cgcgtaggcc gcggagtccc cccgtttctc gtacaaggcg
     1681 gtggagccct tgatgtccgt gaacatgatg cagacctggc ctacgctcag gctctcccga
     1741 agggagagcg tctccgtctc gaagatctcg cggaagagcg ggacgtgcag gcagtccatg
     1801 ccggagacga acaaaggggg aagctggatg gccttcttgc cgtgcagctc gaccgtgcgg
     1861 cgggtctgct cggcaaggaa gcgcttgtcc acggaccaga gccggacggt cggcgtgaag
     1921 gtgacctcca cgttgcggtc cagcgtgttg cggaagtcca ggttgcagag ggggcagtgg
     1981 ttgcctgcgg tagcctcgtt gaggtggcga tgggttccgg caaccgcatt gcagtccgtg
     2041 cagtggaagt cccagttgag gtccacgacg ccgtccttca ccagccggat gaacgtggcc
     2101 aggagcctgg cgcggtccac gcccagggtg tttgccagcg cgtggctgtt gatccgccag
     2161 agatccgcgt cgccggccct ctggaaccag tccaccaggg gcgtccccgg gtagtagcgg
     2221 tccggaccgt cccgcatcag cctcgagatc tcccgagcga tgttcttccc gttcattgct
     2281 tccctcctgc gtagttccta cggtggaata tcgcgcttgg atgtcacgga agcatcacga
     2341 aaatcatcaa gcggctggca atgccaccct ccggctgata gactcaacag caaaggagat
     2401 catttcattg cagcgacaga gcataccgtt cgaccggttg cggcttcccg tggtgtctct
     2461 ctgggatcga cagtggctcc tcctggcaac gggggacttc gaggccgggg agtacaactg
     2521 catgacggta ggctggggga gcttcggggt catgtgggga cgccctcacg ccgtggtggt
     2581 ggttcggccg acccggttca cctggcgcct catggagcgc tcggacagct tcacgctcac
     2641 cgcgttcgcg gaggagcacc gccggaagct ctcctactgc ggcagccact ccggtcggga
     2701 cggagacaag gctaaggcct gcgggctgac cgcttgcgcc gccggccacg ttcgggcgcc
     2761 ggcgttcgag gaggcggagc tgatcgtgga gtgccgcaag atctaccagc acgacttcga
     2821 cccggcccgc ttcctggacc ccaccatcga aaccaactat cccaagaagg attaccaccg
     2881 ggtgtactac gggcagatcc tcgcggtatc ggggatcgcc gcgtaccgga tggaggagta
     2941 acggtatgga catgacgctc ccgctgaaac acccgaagcc cgactccagc tacttcctgg
     3001 acgtcgtgat ggggagaaga agcggccctt cgccgctggt ggaatacctg gtggacgacg
     3061 tggtgatgaa gcccatcgtg gaggggctcc tgggcaggga gtgggcgggt gagggatccg
     3121 tcggcacggc ccacgaagtg cgccgggggg tggtggaccg tgccgcccag acacggtacc
     3181 tcgacaccta catcgacttc tggcttcgca tggggtatga cttcgtgcgg ttcgagcggg
     3241 acgtcggctt cccggaacgc cacctggaga cctccgacac ggcgcccggc tcggccaagc
     3301 gacgcgcctg gtccgatcag cacaccggtt cgatcatgag ctggaaggac tacgagaagt
     3361 atccctggcc ccgcctggag agcttcgact tcttcccgtt cgagtatatc aacagccatc
     3421 taccggacgg catgggcatg atcacctgcc acggcggagg cctgtacgag cacctgtcct
     3481 ggatcatgtc ctacgagggt ctctgcctgg cgctctacga cgcgccggac ctggtcaagg
     3541 cggtggcgga gcgggtcggg agcctcctgg tcgggttcta tcggcacctg ctggacctgg
     3601 accgtgtcat cgccatcttc cagggcgacg acatgggctt caagaccgga acgctcctgg
     3661 cccccgaggc gcttcggacc tactgcctgc cgtggcagag aaggctggcc gcgatgtccc
     3721 acgagcgtgg acgaccgtac ttcctgcact cctgcggcaa cctggccagc atcatggagg
     3781 acctcatcga ggacgtgagg atcgacggca aacactccta cgagaacacc atcatgccgg
     3841 tgaccgagtt ccagcagaag tacgggaccc ggatcgcggt tctcggaggg ctggacatca
     3901 acatcctcac ccgatccacg ccgcaggaga tccgcaagca gaccgcgaac ctcattgagg
     3961 tctgcggcgc ccacggccga tacgcggtgg gatccgggaa ctccgtgccc agctacatcc
     4021 ccgttgagaa ctacctggcg atggtggatg aagcggtgaa gcggcgcggg ctttcctgag
     4081 gctgccgtcc gccgcgtcgg ggaggcagcc gggccctatc cttcgcgcct cgggggcaac
     4141 ggcaggaacg ccgaacgcag ccgcatttgc tccgcgtagc ccgggtggcc cgcggtcatc
     4201 cgacgatcca tcatgggcac gctcacgccc aggaagagag ccgtgatacc gaggggaccc
     4261 acgatggtcc accaccgtga cgggtctgcc gccagaccga acaggtagag cccccaccag
     4321 aagaggacct ctccgaagta gttgggatgc cgcgtgaggg cccagagacc cgtctccagc
     4381 gtggagcccg gctcacgagg ggaatccagg aacgcccgaa gctgtcgatc cgccgtggac
     4441 tcgatcagga tggcggccag ggtcactgcc acggcaacgg catccagcgc gcccacgggt
     4501 cgggcattcc acgccagcgc cgcggtcagg ggcagacacc ccaggaacac gatgatcgtg
     4561 gggaagaggt ggaagcccag gaagctcacg ggccagtagc cggccccgag ccgccggtag
     4621 tccgcgtagc gccagtcctc gtccgagagc ccgcgccagc gccgcagcca gttggccgtg
     4681 agccgcagcg cccagagcgc cacgcacgcg agcgccagca gggcccgagg ccgagccggg
     4741 ccgtacgatg ccagccagaa gccggcgatg aataccggcg ccacgctcca gtacggatcg
     4801 tacacgcttg agttgtcgag cgccgcgctg aatgcgaaca cgaccaccgt ggccgccagg
     4861 tcacccgcgg ccgccatcca gaccgcggag tgccaaccca ggaaccggcc aacggcccaa
     4921 ccggcggcca ccgctgccat ggcggagatg aggtacgccg tgaggcacag tagcagcgat
     4981 tcggaacggc cgagctggga tcggggcgcg ggcagcggca tgatgcttcc tctcgactct
     5041 accagggaat ggcctgcttg cggaccgccg gctggcacgt ggggcagaag aaggaatcgt
     5101 accccaggac gccggcgcgt cggatcgtcc ctccgcacac ggggcagggc tggtcctttc
     5161 ggtttcgcac ccgcatgtgg tctcggactt tctcctcaat gggccgatcc gcctttcgca
     5221 cctcctcgat gccccaggcc atcaccgagc ggatgctgtc gtagagcttg cgccgctcct
     5281 ccgcggagag gctggcgcag gaggtcttgg ggtggatccc ggcggcaaag aggatctcgt
     5341 ccgcataggc gtttccgatg gcgctcagag cggtctggtc catcacgaat acgcgagcct
     5401 ggt
//
LOCUS       DASRZS010000270         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_921766, whole genome shotgun sequence.
ACCESSION   DASRZS010000270 DASRZS010000000
VERSION     DASRZS010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921766"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1744
                     /locus_tag="VFH83_06665"
     CDS             239..1744
                     /locus_tag="VFH83_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET6486083.1"
                     /translation="MKKYLGDILIEKGYISRHDLSRALTYQMHKVVGKDTRKAGAAGF
                     LLDVARTKYNNRDEFYLGKILTELKLLPETKVREALAIQAALPDEKPAARLQALNQVM
                     RRMNSSYNLIDLLNQILVLASTLVEAEATSLIVNDHVTDNLVILMPVGQDAESVRELV
                     IPKDKGIAGWVYRNSRSVIVNEVGKDPRFYAAIDAASGYTSRQILCVPLSVKGRKLGA
                     IEALNKLPVPGAEGSSGFSTEDQIVLEMFAAQAGVAIESTHLTLALSRAQEDLALQRG
                     QMGSVQRTQAAAQVADSFLGEMRRSLIPLQGYAQRMQQAAKDDRVEKYRTYLDEEMDR
                     LITHADNVVRFLRDAFVPARRTIDLREILHELESRLWVSCRVAGIAFEAVAEGDLVVN
                     VDPELTLTVLDNLFRNSRDAMTDGGIFSIHVVRLDDRTVGLEVSDTGTGILVDPPSLI
                     FEAFFSSGKVHAAGLGLCIARKIVEVHGGTLDLGQKPVGSGALFIIRLPVT"
     gene            complement(1751..>2138)
                     /locus_tag="VFH83_06670"
     CDS             complement(1751..>2138)
                     /locus_tag="VFH83_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6486084.1"
                     /translation="VDTLICNTSELEGLVGSGPHGDVERAAHAILGQGVRSVVVTLGG
                     KGCLSVGPQGVTRQPAFPVQAVDTVGAGDCFSGVFAAALAQGADLTAALRLASAAAAI
                     SVTRKGAQPSMPNREEIEEFLRSRPA"
BASE COUNT          385 a          721 c          704 g          328 t
ORIGIN      
        1 gacaagggag actgggtcat ctacaagatc gatcgcctga gctcggatcc tctgcccgcc
       61 ccgcagtagg ctccagaccg tagcggaccg ctcctggtac ccggcgggcg cttcgggccc
      121 cagggggccg ctccggcatc ctgtggacgc ataacgcctt cgaccggcgg cagtgcgtca
      181 agtcggggct catttgtcga ttccgggctg gcgcgctata atcaggccaa aggctcccat
      241 gaagaagtac ctgggcgata ttctcatcga gaagggctac atcagccgcc acgacctgag
      301 ccgtgccctc acctatcaga tgcacaaggt ggtaggcaag gacacccgaa aggccggcgc
      361 cgcgggtttc ctgttggatg tagccaggac caagtacaac aaccgtgacg agttctacct
      421 cgggaagatc ctgaccgagc tcaagctgct tccggagacc aaggtgcgcg aggcgctggc
      481 catccaggca gcgctgccgg acgagaagcc ggccgcccga ctgcaggccc tcaaccaggt
      541 catgcgtcgg atgaacagct cgtacaacct gatcgacctc ctcaaccaga tcctggtcct
      601 ggcctccacc ctggtggagg cggaagcgac gagcctcatc gtcaacgacc acgtgacgga
      661 caacctcgtg atcctcatgc cggtgggcca ggacgcggag tcggtccgag agctggtcat
      721 ccccaaagac aagggcatcg ctggatgggt gtatcgaaac tcccggtccg tgatcgtcaa
      781 cgaggtgggg aaggacccga ggttctacgc ggccatcgac gcggccagcg gctacaccag
      841 tcgacagatc ctgtgcgtcc cgctctccgt caaaggcagg aagctcggcg ccatcgaggc
      901 gctgaacaag ctgccggtgc cgggcgccga gggctcatcg ggattctcga cggaggacca
      961 gatcgtgctg gagatgttcg ccgcgcaggc gggcgtcgcc atcgagagca cccacctgac
     1021 gctggccctg agccgggctc aggaagacct ggcgctgcag cgggggcaga tgggctcggt
     1081 gcagcggacc caggccgcgg cgcaggtggc ggactccttc ctgggggaga tgcgccgttc
     1141 cctgatcccg ctccagggat acgcgcagcg gatgcagcag gcggccaagg acgaccgcgt
     1201 ggagaagtac cggacctacc tcgatgagga gatggaccgg ctcatcaccc acgcggacaa
     1261 cgtggtccgg ttcctgaggg acgcctttgt ccccgcgcgc cgcaccatcg acctgaggga
     1321 gatcctccac gagctcgagt cccgcctgtg ggtgagctgc cgcgtggcgg ggattgcctt
     1381 cgaggcggtg gcggaaggcg acctggtggt caacgtggat cccgagctga ccctcaccgt
     1441 gctggacaac ctcttccgca acagccgcga cgccatgacg gacggaggca tcttctcgat
     1501 ccacgtggtt cgcctggacg atcggacggt gggcctggag gtaagcgaca cgggaacggg
     1561 catcctggtg gatccgccca gcctcatctt cgaggccttc ttctccagcg gcaaagtgca
     1621 cgccgcgggg ctcggactct gcatcgcccg gaagatcgtc gaggtccacg gcggaaccct
     1681 ggacctgggt cagaagccgg tcgggtccgg cgcgctcttc atcatccggc tgccggtgac
     1741 ctgagccgac tcaggccgga cggctcctca ggaactcctc gatctcctcg cggttcggca
     1801 tggacggctg agcgcccttt cgggtgacgg aaatggcagc cgcggcggac gccagtcgaa
     1861 gcgccgccgt caggtccgct ccctgggcca aggcggcagc gaagaccccg ctgaaacagt
     1921 ctcccgctcc gaccgtatcc accgcctgga ccgggaacgc cggctggcgc gtcacgccct
     1981 gaggcccgac gctgaggcac cccttcccgc caagggtcac cacgaccgat cgcacgccct
     2041 gacccaggat ggcgtgcgcc gcccgttcca cgtccccgtg cgggccggaa ccgaccagcc
     2101 cctccagctc gctggtgttg cagatgagcg tgtccacc
//
LOCUS       DASRZS010000271         7311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2844074, whole genome shotgun sequence.
ACCESSION   DASRZS010000271 DASRZS010000000
VERSION     DASRZS010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2844074"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VFH83_06675"
     CDS             <1..855
                     /locus_tag="VFH83_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET6486085.1"
                     /translation="EVPFRLDVAVPILAAREVDAQEREIGPAVVLGGGLITRFGPHQI
                     RSFALKLASPATAAYRSRPVSLPFDRSVASFDGERANVGFNGQGEALPAELLPGEIAY
                     GSIRFSLGAAGHGRSNALTARGQTIRLPEGRFGRLYILASSADGDRSVSFRIGQTPVS
                     IVIQDWSGFIGQWDDRLWRIVGSDGPGSRFRDDALQGFAGITPGFIKRVPVAWYASHH
                     HSADGGNEPYAYAYLFAYCFDLPSGTSTFTVPNDDSVRILAATASEEAPPSRPARPLY
                     DVLGTADP"
     gene            complement(894..967)
                     /locus_tag="VFH83_06680"
     tRNA            complement(894..967)
                     /locus_tag="VFH83_06680"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(931..933),aa:Arg,seq:ccg)
     gene            1037..2602
                     /locus_tag="VFH83_06685"
     CDS             1037..2602
                     /locus_tag="VFH83_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET6486086.1"
                     /translation="MEPFKRVLWLVIDGLGFEEARRYIQVRPNGPLARLAREGYLGPS
                     TPSSPVCQTPSALVALFSGAQPAQSGVWGYRMPDPRRPRASMLGFYAPVEGLSTVWGE
                     LEERGQGYSVMNAAFRNDPVWSRGKSARFAYDGYRLWKKPTAIRTSSGRQRMDLLGVE
                     MRLDLDATGVGITKGGRLRCQVPLDGTARLQVTPGKEARVFSLGGAGLLICPLTLPGV
                     RGSSPPDGARDGFCDGDAFRFVRSWMDGHGVSTSSRSGGMDVSVAAEMGPTNASFSQK
                     AELMLAAARDPQARLVIGYLPNVDELNHSYADLIEAEWPEGRGSVLLLRTLGLVDTLL
                     DRLMSGAEPGTLLVLSSDHGTVPHRHELYLNELLARAGLVHRCKDGYDPARSVAYYHP
                     ADCGQIVAGGRRAADPLPAIRRALDLASRELHAPIGLQEAGPGDPYLAFAYPLADVEI
                     TGRAPKPGMPILRRKPGGNHLSPLTPTPWIQAVLGAWSPGRNLGAGECPTENVGLKSF
                     VLRALDIASSGRS"
     gene            2609..3190
                     /locus_tag="VFH83_06690"
     CDS             2609..3190
                     /locus_tag="VFH83_06690"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET6486087.1"
                     /translation="METIILASGSPRRRDLLGRVHIPVKVISPNVPEDYSGSAPASEM
                     VARIALSKVEAVLELFKTESPRWVLGVDTVVVVDGNILGKPQDAAAAEVMLGRLSGKV
                     HEVYSGLALLVRRGIAVEQQTVRTEVKFREMTASEMRYYVDTGDWVGAAGGYRIQERG
                     GFFVEWIRGSYSNVVGLPIEAFYGILVRNGFQF"
     gene            3259..4218
                     /gene="rpsB"
                     /locus_tag="VFH83_06695"
     CDS             3259..4218
                     /gene="rpsB"
                     /locus_tag="VFH83_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET6486088.1"
                     /translation="MAVVTMKNLLESGVHFGHQTKRWDPRMKKYIFAERNGIHIIDLQ
                     KTIVAIKDAYEAVRKSVLAGKAVLFVGTKKQAQQTVEREAKRCNMFYVNNRWLGGMLT
                     NFATIKKSLLRLKKLEKMEIDGTFENLTKKEIARLLKERAQLEKNLGGIKEMKELPGI
                     VFVIDTRKEDIAVAEARKVNVPIVAVVDTNCNPEGITFPIPGNDDAIRAINLFAQIIA
                     NAVIEAENEVGLEVVETLQEEKGEEEFAEAIDKGEFAAQSKYDQEEEKVAVAAALGAA
                     SDESATGFTDKDYSNYNPDEAAKKDEPPAVEEPEIDEDKLYKE"
     gene            4230..5072
                     /gene="tsf"
                     /locus_tag="VFH83_06700"
     CDS             4230..5072
                     /gene="tsf"
                     /locus_tag="VFH83_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET6486089.1"
                     /translation="MEVKASDVKQLREKTGAGMLDCRNALAKTEGDFGKAERLLKEQG
                     LAAAVKKSERVTNSGKIFSRILPDRGILLELTCETDFVARNSLFLELGDAILSAVASQ
                     NLSGPTPELELMVQEAVGKIKENMQLRRIVTLSVGADEALMEYVHDDRIGVMLRFTVG
                     KPELKQDPRAKALFFDCALHAAAFAPLFLSRDKVGADFLKEQEEIFTKQAQGLNKPAN
                     VLQGIIKGKVNKMLSEICFLDQPFVKDDKRSVAKVLQDFSKEIGAPVAIADYRYVKLG
                     ENRS"
     gene            5086..5646
                     /gene="frr"
                     /locus_tag="VFH83_06705"
     CDS             5086..5646
                     /gene="frr"
                     /locus_tag="VFH83_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET6486090.1"
                     /translation="MTRDKIRSQAEEKMKKAIQSLQDEFKTLRGSRASQSLFEKIRVD
                     YYGKKTPLNQVATISIPEARMVIIQPWDRSVLSEIEKAIQKSELSVNPNNDGKVIRIN
                     IPPLTEERRKDLVKVAKNTAEQSRVAVRNVRRDANEEIKKAKSDSELSEDEAKKATDE
                     IQKLTDRSIEEVNKILEAKEKEILEV"
     gene            5643..6365
                     /gene="uppS"
                     /locus_tag="VFH83_06710"
     CDS             5643..6365
                     /gene="uppS"
                     /locus_tag="VFH83_06710"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET6486091.1"
                     /translation="MSPSPDWSVLDPERLPRHVGIIMDGNGRWARARGKARTEGHLEG
                     VKAAKRVVAAAAEARLPFLSLYTFSTENWNRAQEEVSYLMFLVRSYLRKEYAFYRENG
                     IRIVHSGDAQGLPRDVRDELEEVMRDTAGFTGLTVNLSINYGGRDEILRAFRRASAAE
                     GEGGLTEDGFRRYLDLPEFPDPDLIIRTSNEHRLSNFLLWQSAYAELYFSPKLWPDWD
                     QGDLYEALVEYGRRARRFGGAP"
     gene            6362..7210
                     /locus_tag="VFH83_06715"
     CDS             6362..7210
                     /locus_tag="VFH83_06715"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET6486092.1"
                     /translation="MNWRGFLSRIALVAVAFPVLAALIFLVPQASHLGFNIVVLIVSV
                     IGGLETAALFHARGIPTSRYLAAALSATIPAGTYLEVVGLVPVGWMGVWVPFLLAVLL
                     VRGILFQGRKELPSILAFVSSSTLTLLYPGFFMSWIVRLSGLQQPSVTILFFLCLVFG
                     NDMSAYFAGSLWGESTRLGLPISPQKSVVGFGAGLVGSLIIVSFFLPVRGFLPYPPAG
                     SYLLGVVVGLVVMLGDLLESGLKRSAGVKDSGIVIPGRGGVLDSVDSMVLSAPLFYYA
                     FVLAGR"
BASE COUNT         1335 a         2270 c         2416 g         1290 t
ORIGIN      
        1 gaggtcccgt tccgcctgga cgttgcggtc cccatcctgg ccgcgcggga ggtcgatgct
       61 caggagcgtg agatcggacc agcggtcgtc ctcggagggg ggctgatcac gaggtttggg
      121 ccgcaccaga tccgttcctt cgcgttgaag ctcgcatccc ccgcgacggc ggcctatcgc
      181 tcgcggcccg tttcccttcc gttcgaccga tccgttgcga gcttcgatgg agagcgggcc
      241 aacgtcggct tcaacggcca gggagaggcc ctgcccgctg agctgctacc gggggagatc
      301 gcctatggga gcatccgctt ctcgctgggc gccgcgggcc acggacggtc caatgccctg
      361 accgcccgcg gccagaccat ccgtctcccc gaggggcggt tcgggcggct ctacatcctg
      421 gcgtcgtcgg cggatgggga ccggagcgtc agcttccgga tcgggcagac gccggtctcg
      481 atcgtcatcc aggactggag cggtttcatc gggcagtggg atgaccggct gtggcggatc
      541 gtcggtagcg acggtcccgg gagccggttc cgggatgacg cgcttcaggg cttcgccgga
      601 atcacgcccg gattcatcaa acgcgtgccc gtcgcctggt acgcatccca ccatcattcc
      661 gctgacggcg gcaacgagcc ctacgcgtat gcctacctgt ttgcttactg ctttgatctg
      721 ccatctggga cgagcacgtt cacggtcccc aatgacgatt cggtgcggat cctcgcggca
      781 acggcttcag aggaggcacc gccaagcaga ccagctcgac ctctctatga tgttctggga
      841 accgctgatc catgaagaag gcccgcaggg tgcccgccct tgcggcgaag tagtgggcaa
      901 tgcgcgattc gaacgcgcgg cctatggctc cggaggccat cgctctatcc aactgagcta
      961 attgcccaaa cgcgccagac gtgcaattct gccgcttgcg ctgagtgaac gatactcgca
     1021 aggcggccat ttgtcaatgg aaccattcaa gagagtactc tggctggtca tagacggcct
     1081 cggcttcgaa gaagccaggc gctacatcca ggtccgaccg aacgggccgc tggctcgtct
     1141 ggcgcgggaa ggctatcttg ggccttccac tccttcctca cccgtctgcc aaaccccgtc
     1201 cgcgctcgtg gctctcttct ccggggccca gccggcccag agcggggtat ggggctaccg
     1261 gatgccggac cctcgtcggc cccgggcctc gatgctcggt ttctacgcac cggtggaggg
     1321 tctgagcacc gtgtggggag agctcgaaga gcgaggccag ggctactccg tcatgaacgc
     1381 ggccttccga aacgacccgg tatggtctag gggaaagagc gcccgcttcg cctacgacgg
     1441 gtaccgtcta tggaagaagc cgaccgccat ccggacctcg tccggccgtc agcggatgga
     1501 cctcttgggt gtcgagatgc ggctggacct ggatgcgacc ggcgtcggca tcacgaaggg
     1561 cggacggttg cggtgccagg tcccgctcga cggcactgcg cgtctccagg tgactcccgg
     1621 gaaggaggca cgggtgttct ccttgggtgg ggccggcctg ctgatctgcc cgcttacgct
     1681 tcccggcgtg cgcgggtcct ctcctcccga tggcgcgcgt gacggcttct gcgacggcga
     1741 cgcatttcgg tttgtccgat cctggatgga cggccatggc gtctcgacat cctcccgctc
     1801 gggagggatg gatgtctcgg tggctgccga gatggggcca accaatgcca gcttcagcca
     1861 gaaggcggag ctgatgctcg cggcggccag ggatccgcag gcgcgtctcg tcatcggcta
     1921 cctcccgaac gtcgacgagc tgaaccactc ctacgcggat ctcatcgagg cggagtggcc
     1981 cgagggccgg ggatcggtgt tgcttctgcg gaccctgggg ctcgtcgata ccctgctgga
     2041 ccggctgatg tcgggcgcgg aacccggcac cctcctggtc ctctcatcgg accatggcac
     2101 ggtgcctcat cgccatgagc tttacctgaa cgagctcctg gcgcgggccg gcctggtgca
     2161 ccgatgcaag gatggctacg atccggcccg atccgtggcc tactatcatc ccgcggactg
     2221 cgggcagatc gtggcgggcg gcaggcgtgc cgctgatccg cttccggcga tccgccgggc
     2281 gctggacctg gcttcgcgcg agctccatgc gcccatcggc ctccaggagg caggtccagg
     2341 ggatccatac cttgctttcg cttaccccct ggccgacgtc gagatcacgg gtcgggcgcc
     2401 gaaaccgggc atgccgatcc tccgccggaa gccgggcggg aatcaccttt cccctctcac
     2461 accgaccccc tggattcaag cggtgttggg ggcctggtcg ccgggccgaa acctgggagc
     2521 cggggaatgc ccgacggaga acgttgggct caagagtttc gttcttcgcg cgctggacat
     2581 cgcgtcgagc gggaggtcct gaaccatcgt ggaaaccatc atccttgctt ccgggtcgcc
     2641 cagaaggcgg gatctccttg gccgcgttca cattccggtc aaggtgatct ctcccaacgt
     2701 gccggaggat tactccggct ccgccccagc gtcggagatg gtggcgcgca ttgcgttgag
     2761 caaggtggaa gcggtcctgg agctcttcaa gaccgaatcc cctcgatggg ttctcggcgt
     2821 ggacaccgtc gtggtggtgg acgggaacat ccttgggaag ccccaggacg ccgcggccgc
     2881 cgaggtgatg cttggccggc tctccggcaa ggtccatgag gtgtactcgg ggctggcgct
     2941 cctggtccgc cgcggcatcg cggtggagca gcagaccgtc cgcaccgagg tcaagttccg
     3001 tgagatgacc gcctccgaga tgcgctacta cgtcgacacg ggcgactggg tcggggcagc
     3061 gggcggctac cgcatccagg agcgcggcgg attcttcgtg gagtggatcc ggggctctta
     3121 cagcaacgtc gtaggcttgc caatagaggc gttttatggc atcttggtcc ggaacggctt
     3181 tcaattctag ccctttcatc tcacccgcca gtctcggatg ggtgtaaaag ccaaggagag
     3241 aggggaagga gggtcatttt ggctgtcgtt accatgaaaa acctgctcga atccggtgtg
     3301 catttcggcc accagaccaa gcgctgggat ccgcggatga agaagtacat cttcgcggag
     3361 cgcaacggca tccacatcat cgatctgcag aagacgatcg tggccatcaa ggatgcctac
     3421 gaggcggtgc gcaagagcgt cctggccgga aaggccgtgc tgttcgtggg caccaagaaa
     3481 caggcccagc agaccgtgga acgggaagcg aagcgctgca acatgttcta cgtgaacaac
     3541 cggtggctcg gcggaatgct caccaacttc gcaaccatca agaagtccct cttgaggctg
     3601 aagaagctcg agaagatgga gatcgatggg acgttcgaga acctcaccaa gaaggagatc
     3661 gcccgactgc tcaaggagcg cgcccagctg gagaagaacc tgggcgggat caaggagatg
     3721 aaggagctgc cggggatcgt ttttgtcatc gacacccgca aggaggacat cgccgtcgcc
     3781 gaggcgcgca aggtcaacgt gccgatcgtg gcggtggtgg acaccaactg caaccccgaa
     3841 ggcatcacgt tcccgattcc gggaaacgac gacgccatcc gcgccatcaa cctgttcgcc
     3901 cagatcatcg ccaacgcggt gatcgaggcg gagaacgagg tgggcctgga ggtcgtggag
     3961 acgcttcagg aggagaaggg cgaggaggag ttcgcggagg ccatcgacaa gggagagttc
     4021 gccgcgcagt ccaagtacga tcaggaggaa gagaaggtcg cggtggccgc cgccctgggc
     4081 gccgcctcgg acgagtcagc gaccggcttc acggacaagg actactccaa ctacaacccg
     4141 gatgaggcgg ccaagaagga cgagcccccc gcggtcgaag agcccgagat cgacgaagac
     4201 aagctataca aggagtgaga ttcagtccca tggaagtaaa ggcatcggat gtcaagcagc
     4261 tccgggagaa aaccggggcg ggaatgctgg attgccgtaa cgcgctggcc aagaccgaag
     4321 gggacttcgg caaggcggag cgcctgctca aggagcaggg gctggccgcc gccgtgaaga
     4381 agagcgagcg cgtcaccaac agcggcaaga tcttctcgcg catcctcccg gaccggggca
     4441 tccttctgga gctcacctgt gagacggact tcgtggcccg caatagcctg ttcctcgagc
     4501 tcggggacgc catcctgagc gcggtagcct cccagaacct cagtgggcca acgccggagc
     4561 tcgagctcat ggtccaggag gccgtgggca agatcaagga gaacatgcag ctgcgccgga
     4621 tcgtcacgct ctccgtcggg gccgacgagg ccctcatgga gtacgtgcat gacgaccgca
     4681 tcggggtcat gctgcgcttc acggtcggca agcccgagct gaagcaggac ccgcgggcga
     4741 aggcgctgtt cttcgactgc gcgctgcacg cggcggcgtt cgccccgctc ttcctctcca
     4801 gggacaaggt gggggcggac ttcctcaagg agcaggagga gatcttcacc aagcaggccc
     4861 aggggctgaa caagcctgcc aacgtcctgc aggggatcat caagggcaag gtgaacaaga
     4921 tgctttcgga gatctgcttc ctggatcagc ccttcgtcaa ggacgacaag cgcagcgtcg
     4981 cgaaggtgct gcaggatttc tccaaggaga tcggcgcccc ggtggccatc gcggactacc
     5041 gatacgtgaa gctcggcgaa aaccgttcct gaaggagggt cgggaatgac tcgggacaag
     5101 atccgaagcc aggcagagga gaagatgaag aaggccatcc aatccctgca ggacgagttc
     5161 aagaccctga ggggaagcag ggcgtcccag tccctgttcg agaagatccg ggtggactac
     5221 tacggcaaga agacgccgct caaccaggtc gccacgatct ccatccccga agcccgcatg
     5281 gtcatcatcc agccatggga tcgctccgtc ctttcggaga tcgagaaggc cattcagaag
     5341 tcagagttgt ccgtcaatcc caacaacgac ggcaaggtga tccgcatcaa cattccgccg
     5401 ctcaccgagg agcgccgcaa ggacctggta aaggtcgcca agaacacggc ggagcaatcc
     5461 cgggtcgccg tgcgcaacgt ccgtcgggat gcgaatgagg agatcaagaa ggccaagtcg
     5521 gactccgagc tcagtgagga cgaggccaag aaggccaccg acgagatcca gaagctgacg
     5581 gaccgctcca tcgaggaggt gaacaagatc ctcgaggcca aggaaaagga aatcctggag
     5641 gtgtgagccc ctcgccggac tggagcgtcc tggatcccga gcggcttcct cgccacgtcg
     5701 ggatcatcat ggacggcaat ggccgttggg ccagggcgag ggggaaggct cgtacggagg
     5761 ggcatctgga aggcgtgaag gccgccaaac gcgtggtggc cgcggccgcg gaggcccgtc
     5821 ttcccttcct gagcctctac accttctcca cggagaactg gaaccgcgcc caggaagagg
     5881 tgtcctatct catgttcctg gtgcgctcgt atctgaggaa ggagtacgcc ttctaccggg
     5941 aaaacgggat ccgcatcgtg cacagcgggg atgcccaggg gcttccccgg gacgtgcggg
     6001 atgagctgga agaggtcatg agagacacgg caggattcac cggtctcacg gtcaaccttt
     6061 cgatcaacta cggcggacga gacgagatcc tgagggcgtt ccgcagggcc tccgccgcgg
     6121 agggggaagg cgggttgacg gaggacggct tccgcaggta cctggacctt cccgagttcc
     6181 cggatcccga cctgatcatc cgaaccagca acgagcaccg cctgtccaac ttcctcctgt
     6241 ggcagagcgc ttacgcggag ctgtacttca gcccgaagct ctggcccgac tgggaccagg
     6301 gcgacctcta tgaggccctg gtggagtacg ggcgccgagc ccggcgcttt ggaggagccc
     6361 catgaactgg cggggcttcc tgtcccgcat agctctggtg gccgtggcgt tccctgtgct
     6421 ggccgccctc attttcctgg ttccgcaggc cagtcacctc ggattcaaca tcgtggtgct
     6481 gatcgtcagc gtcatcggag ggctggagac ggccgccctc ttccacgccc ggggcatccc
     6541 aacgtcccga tacctggccg ccgcgctcag cgccaccatc cccgcgggga cgtacctgga
     6601 ggtggtgggt ctggtcccgg tgggatggat gggggtatgg gtgccgttcc tcctcgcggt
     6661 cctcctggtg cgaggcattc tcttccaggg acgcaaggag cttccatcga tcctggcctt
     6721 cgtgtcctcc tccacgctca ccttgctgta ccccggtttc ttcatgagct ggatcgtgcg
     6781 tctctcgggc ctccagcaac cgtccgtcac gatcctgttc ttcctctgtc tggtcttcgg
     6841 aaacgacatg tccgcctact tcgccgggag cctctggggc gagtccacgc ggctgggcct
     6901 ccccataagc ccgcagaaga gtgtggtcgg cttcggggcc ggactggtcg gctccctgat
     6961 catcgtgagc ttctttctcc cggttcgcgg cttccttccg taccctccgg ccggcagcta
     7021 cctgttgggc gtcgtagtcg gcctcgtcgt gatgctcggc gacctgctgg aaagcgggct
     7081 gaagcgctcg gccggagtca aggattccgg gatcgtcatt ccgggacggg gcggggtcct
     7141 ggattcggtt gactccatgg tgctgagcgc cccacttttc tactatgcgt ttgtactggc
     7201 gggtcggtag gggatgagat gacggctctc accatcctcc tgggaatcat cggtctcggt
     7261 ctcatgatgt tcatccatga gctgggccac tttgtcgccg caaagatcaa c
//
LOCUS       DASRZS010000272         5272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5541991, whole genome shotgun sequence.
ACCESSION   DASRZS010000272 DASRZS010000000
VERSION     DASRZS010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5541991"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VFH83_06720"
     CDS             <1..930
                     /locus_tag="VFH83_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6486093.1"
                     /translation="VEELSASVEQVAGNAQSQATSGEQGSSAIAQVRTSIEEMSRNLS
                     GISQLASQSVEKSVEGAQAVQKVVEAITQISQGSDRIAGIVTVISDIADQTNLLALNA
                     SIEAARAGEHGRGFAVVAEEVSKLADRSASSTKEIEALIKESVTSVTSGVRIAQGSHE
                     AMGQIRGASQQVKSMIVELSRAMDSQVATLKDLVSAIQNIGDMSQSISAATEQQTVNA
                     KEVAKAMESVSDLTQSSASAAEQMSASTEELTGMARQLRDMIAGFKTGVNELRQAETA
                     RQAGNGQRVVQPASPAEDGQEPTTYREEARLAG"
     gene            1057..2328
                     /locus_tag="VFH83_06725"
     CDS             1057..2328
                     /locus_tag="VFH83_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6486094.1"
                     /translation="MRKASKVLCCLPMLLCIVLLGLGSNAWAAPQKVVFWKAPHSDQE
                     AVYWKPILDKFMKENPGITVEHLVTPWDTWTEKYTAAFAGGNPPDVSYMTEWYPTFAE
                     ANQLADLSTYVSADTKKAIGKGNWDYATYKGKLIGIPFLAVDSIVFYNKDIFAKEGVS
                     IPKTWDEFLAVAKKLTKDTNGDGKIDQWGTAFGLKPEVNIHQFMPFVIAAGQTYLSAD
                     EKQLGFANPDGIKAIKYITDFIVKEKVAPTIDMETDQNWASNLFYSGKVAMVIGQNIF
                     ESDVKTNAPNLKLGAFEIPASSVKDPRVARANYGGLGLLSMAQASKNKDLAWKLIDFI
                     MRPENASMYLSKVNFLSPVESANKIMYPSDEIINVASAATQSMVNYPVNPKWKEIDTT
                     LKLMLEEIIRGAKPADKAVMDANEKAKAILK"
     gene            2420..3301
                     /locus_tag="VFH83_06730"
     CDS             2420..3301
                     /locus_tag="VFH83_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6486095.1"
                     /translation="MPFFRRRKVRESLSAYGFILPFAVFCCAFAILPVLSVFKLSFQT
                     GGFLDKPVWVGLSNYAGIFADPQYLGYFLNNLVYIAFSIPFGQFFAFTLALLLKEKTR
                     ASAFFESVFFLPLLLSMVAAGTIITYVFNLNGPVNYVLGLFRLEPVNWLGQPFTAKIT
                     IVIAELWKGATFYTFVYIAALRAVPAEYFEVGHMEGARWLQMLRRVTLPLVKNTILFC
                     FVMTTIWQLQIFDSIYVMTSGGPMNTTMSVVFSIYQTTFKHFHVGPGSALSILFLIVI
                     LVISLIQMSLSRSDVEY"
     gene            3315..4160
                     /locus_tag="VFH83_06735"
     CDS             3315..4160
                     /locus_tag="VFH83_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6486096.1"
                     /translation="MERTGRSTRAIARTGKWTLLVIGVLVVLIPFWLLGISSLRQSTV
                     LLEYPPRFWPERATLANYIALFDPERYDFIAWFVNSVIVAGASTLGTVLVCSLAGYAF
                     AKKSFPGKRVIFAIALGTMMIPSAVTLIPLFLIVKGLGLINTLTGIIVPSLGAAFGVF
                     LMRQFISTLPSSLLESAQMDGCRELGVYARIVVPMTTPALAVLSIFTFLNQWNSLVFP
                     LIIADSENKKMLTVGIAGLKSMASSPWGLMMAASLLSFVPIFLVFLFMRERFVAGLTA
                     GAVKG"
     gene            complement(4197..5060)
                     /locus_tag="VFH83_06740"
     CDS             complement(4197..5060)
                     /locus_tag="VFH83_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET6486097.1"
                     /translation="MRTPPSPRRPEREHLLPFADSLKRLPFWLLGIILLGVLTLWSIV
                     SRSDYRVIFRATAGGLLTTLWVSLVAFGFAIALGLLLGLCRVSPFRPIREVATFIVEI
                     VRGVPMLVILYYIAFIGAPGLVQAVNFLLGPLTRAGILPPITVRSISFSWRAIIALTL
                     GYGAFLSEIFRAGIESIPAGQWEAAYALGLRRGQAMSRIVLPQAIRNVLPPLGNEFVA
                     MIKDSALVSALGVQDVTQMGKVYAAGTFKFFETYNIVAFLYLFLTITLSMLVRLLERR
                     LKTGRTATHLR"
     gene            complement(5134..>5272)
                     /locus_tag="VFH83_06745"
     CDS             complement(5134..>5272)
                     /locus_tag="VFH83_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013383086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HET6486098.1"
                     /translation="EDYGFIFKLGSDLVVPFNAALKSMKADGTLDSFTNTWFTVYGTQ
                     P"
BASE COUNT         1010 a         1687 c         1668 g          907 t
ORIGIN      
        1 gtggaggagc tcagcgcttc cgtggagcag gtggccggca acgcccagtc tcaggccacc
       61 tccggggagc agggatccag cgccatcgcg caggtgcgca catcgatcga ggagatgtcc
      121 cgcaacctct cggggatctc ccaactcgcc tcccagtcgg tggagaaatc cgtggaagga
      181 gcccaggccg tccagaaggt ggtggaagcc atcacgcaga tctcccaggg gtccgaccgg
      241 atcgcgggca tcgtcacggt gatttccgac atcgcggacc agaccaacct cctggccctc
      301 aacgcgtcga tcgaagcggc gcgcgccgga gagcacggcc gcggcttcgc cgtcgttgcc
      361 gaggaggtga gcaagctggc cgaccgcagc gcatcctcaa ccaaggagat cgaggccctg
      421 atcaaggaga gcgtcaccag cgtgaccagc ggggtccgaa tcgcccaggg ctctcacgag
      481 gccatggggc agatccgcgg cgcgtcccag caggtgaaga gcatgatcgt ggagctctcg
      541 agggccatgg atagccaggt ggccacgctg aaggacctgg tgagcgcgat ccagaacatc
      601 ggagacatga gccagagcat ctccgcggca acggagcagc agaccgtcaa cgccaaggag
      661 gtcgcgaagg ccatggagag cgtgagcgac ctcacgcaga gctcggcctc cgccgcggag
      721 cagatgtcgg cgtccacgga ggagctgacg ggcatggccc ggcagctgcg ggacatgatc
      781 gcggggttca agaccggggt gaacgagctt cggcaggctg agaccgcccg ccaggccggg
      841 aacggacaga gggtcgtcca gccggcttcc ccggctgagg acgggcagga gccgacgacg
      901 taccgagagg aggcgcgcct ggcggggtag cccatgtaat gttgacttcc gggaggtttg
      961 ggcacagaat cgtaccggcc cgcgtgcggg cggcccgcgt acgggcggcc cgcatgcctg
     1021 ccacgtcagg gcgtctgacg aaaggagaaa ggaaccatga gaaaagcgag caaggtcctc
     1081 tgctgcctgc cgatgcttct gtgcatcgtc ctcctgggac tcggatcgaa tgcctgggcg
     1141 gccccgcaga aagtggtctt ctggaaggcc cctcacagcg accaggaagc cgtctactgg
     1201 aagccgatcc tcgacaagtt catgaaagag aaccccggca tcacggtgga gcacctcgtc
     1261 acgccctggg acacctggac ggagaagtac accgcggcat tcgccggcgg caacccgccg
     1321 gacgtgtcgt acatgaccga gtggtacccg accttcgccg aggccaatca gctcgcggac
     1381 ctgagcacct acgtgtccgc ggacaccaag aaggccatcg ggaagggcaa ctgggattac
     1441 gcaacctaca agggcaagct gatcggaatc cccttcctgg cggtggactc catcgtcttc
     1501 tacaacaagg acatcttcgc caaggaaggc gtctccattc cgaagacctg ggacgagttc
     1561 ctggcggtgg ccaagaagct taccaaagac accaacggtg acgggaagat cgaccagtgg
     1621 gggaccgcct tcggtctgaa gcccgaggtc aacatccacc agttcatgcc gttcgtcatc
     1681 gccgcggggc agacgtacct cagcgcggat gagaagcagc tcggattcgc caatccggac
     1741 ggcatcaagg ccatcaaata catcacggac ttcatcgtga aggagaaggt ggcgcccacc
     1801 atcgacatgg agaccgacca gaactgggcc agcaacctgt tctactccgg caaggtggcc
     1861 atggtgatcg ggcagaacat cttcgagtcg gacgtgaaga caaacgcccc caacctcaag
     1921 ctgggcgcct tcgagattcc ggccagctcg gtgaaagacc cccgcgtggc tcgcgccaac
     1981 tacggcggtc tgggactcct cagcatggcc caggcaagca agaacaagga cctggcctgg
     2041 aagctcatcg atttcatcat gcggcccgag aacgccagca tgtacctctc caaggtcaac
     2101 ttcctgtccc ccgtggagtc ggcgaacaag atcatgtatc cgagcgacga gatcatcaac
     2161 gtcgcctccg ccgccaccca gtccatggtg aactaccccg tgaaccccaa gtggaaggag
     2221 atcgacacca cgctgaagct gatgctggag gagatcatcc gcggcgccaa gcccgcggac
     2281 aaggccgtca tggacgccaa cgagaaggcg aaggccattc tgaagtagcc atgcgtttct
     2341 cggagagact cccccgggcc cgccggcccg gggcgtctcc cggtcgtcgc cgatccaacg
     2401 ggagacgaga agagccgtga tgccgttttt caggcgccga aaggtccgag agagcctttc
     2461 cgcgtacggc ttcattcttc cgtttgccgt cttctgctgc gccttcgcca tcctcccggt
     2521 cctttccgta ttcaagctga gctttcagac gggagggttc ctggacaagc ccgtgtgggt
     2581 cgggctcagc aactacgccg ggatctttgc cgatccccag tatctgggct atttcctgaa
     2641 caacctcgtc tacatcgcct tctcgattcc cttcgggcag ttcttcgcct tcaccctggc
     2701 gctcctcctg aaggagaaga cccgcgcgtc cgcgttcttc gagagcgtgt tcttccttcc
     2761 cctgcttctc tccatggtag ccgccggcac catcatcacc tacgtgttca acctcaacgg
     2821 cccggtcaac tacgtgctgg ggctcttccg cctggagccg gtgaactggc tgggtcagcc
     2881 gttcaccgcc aagatcacga tcgtgattgc cgagctgtgg aagggcgcca cgttctacac
     2941 gttcgtctat atcgccgccc tgcgcgccgt ccccgccgag tacttcgagg tggggcacat
     3001 ggagggggcg cggtggctgc agatgctgcg tcgggtgacg ctccccctgg tcaagaatac
     3061 catcctcttc tgcttcgtca tgaccaccat ctggcagctt cagatcttcg attcgatcta
     3121 cgtcatgacc tccggtgggc ccatgaacac gacgatgagc gtcgtcttct cgatctacca
     3181 gaccacgttc aagcatttcc acgtcggccc gggctcggcc ttgtcgatcc tcttcctcat
     3241 cgtgatcctg gtgatatcgc tcatccagat gagcctttcg agaagcgatg tcgagtactg
     3301 agggggggcg tcggatggag aggaccggcc gaagcacccg ggcgattgcc cggaccggga
     3361 agtggacgct gctcgtcatc ggcgtcctgg tggtgctgat ccccttctgg ctgctgggca
     3421 tctcgtccct ccgccagtcc accgtgctgc tggagtatcc cccccgcttc tggcccgaga
     3481 gggccacgct tgccaactac atcgcgctgt tcgatcccga gcggtatgac ttcatcgcct
     3541 ggttcgtgaa cagcgtcatc gtggcgggcg cctccacgct cggcacggtg ctggtgtgct
     3601 ccctggccgg ctacgccttc gccaagaaga gcttccccgg caaacgggtg atcttcgcga
     3661 ttgccctggg cacgatgatg atccccagcg ccgttacgct catcccgctc ttcctgatcg
     3721 tgaagggact cggtctgatc aacacgctca cggggatcat cgtcccatcc ctgggcgcgg
     3781 ccttcggggt cttcctgatg cgccagttca tctcgaccct tccctcttct ctgctggagt
     3841 cggcccagat ggacgggtgt cgggagctgg gggtctatgc gcggatcgtc gtgccgatga
     3901 ccacgccggc gctcgccgtg ctcagcatct tcaccttctt gaaccagtgg aacagcctgg
     3961 tcttcccgct catcatcgcc gactcggaga acaagaagat gctcacggtc ggaatcgccg
     4021 gcctgaagag catggcgtcc agcccgtggg ggctcatgat ggccgcctcg ctgctgtcct
     4081 tcgtcccaat cttcctcgtg ttcctcttca tgcgagagag gttcgtagcc gggctcaccg
     4141 ccggcgccgt gaaaggctga tgcgacggcg gcctgcgttc cgcgcctgcc ctggcgttat
     4201 ctcaggtgtg tcgcggtccg tccggtcttg agcctgcgct ccaggagtcg gacgagcatg
     4261 gagagggtga tcgtcaggaa caggtagagg aaagccacga tattgtaggt ctcgaagaac
     4321 ttgaacgtgc cggcagcgta caccttcccc atctgcgtca cgtcctgcac ccccagggcg
     4381 ctcaccagcg cggagtcctt gatcatggcg acgaactcgt tgcccaacgg cggcagcacg
     4441 ttgcggatgg cctggggcaa gacgatgcgg gacatggcct ggcctcgtcg cagacccagc
     4501 gcgtatgctg cctcccactg gcccgcgggg atcgactcga tgcccgcccg aaagatttcc
     4561 gacagaaacg cgccataccc gagggtgagc gcgatgatgg cgcgccagga aaagctgatg
     4621 ctgcggaccg tgatcggcgg aaggatcccc gcccgggtaa gcggccccag gaggaagttc
     4681 accgcctgga caagaccggg cgctccgatg aacgcgatgt agtagaggat gacgagcatc
     4741 gggacgccgc ggacgatctc cacgatgaac gtcgccacct cgcggatcgg tcggaacggg
     4801 gacacgcggc agagccccaa caggagaccc agggcgatgg cgaaaccgaa ggccacgagg
     4861 gagacccaga gcgtggtcag gagcccgccg gcggtcgcac gaaagatgac gcggtagtcc
     4921 gagcggctca cgatgctcca gagggtaagc acgcccagga ggatgatgcc caggagccag
     4981 aagggaagcc tcttgagcga gtccgcaaac ggaaggagat gctcccgctc gggtcggcgg
     5041 ggagacgggg gtgttctcat ggggccggcg ggttccccac gggcgggggt gacgggatcc
     5101 ggcggatccc gccccgcccg cgaggaagga tcgtcacggc tgggttccgt agaccgtgaa
     5161 ccacgtgttg gtgaacgagt ccagcgttcc gtccgccttc atgctcttca acgccgcgtt
     5221 gaacgggacc accaggtccg atcccagctt gaagatgaag ccgtagtcct cg
//
LOCUS       DASRZS010000273         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4000714, whole genome shotgun sequence.
ACCESSION   DASRZS010000273 DASRZS010000000
VERSION     DASRZS010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4000714"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1312
                     /locus_tag="VFH83_06750"
     CDS             29..1312
                     /locus_tag="VFH83_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6486099.1"
                     /translation="MLSFLRAQLRSTREVIAGFKGNARALLICEPLWGIPYNLYATYA
                     SVYMLALGCTETQVGLIASCALVSAIVFSLVGGWIIDRMGRKRATLFFDILSWSIPGV
                     LLATSRGFTMFLLAALINSLVRVVNISWDCLMVEDTPPAQRVHFYTWIAVAGILAGFI
                     SPVSGPVVDRFGLVPAMRALYAFAAISMTVMFFWRNSMTHETEIGAMKMRETRHGDAG
                     TTASEYARITGQLLRNPLTLTALVLNLLLTIQMTLKGTFLSILLIRRLGFSAGSISIF
                     PAINSAVVLLVYSLAMPAVGRMKIHRPLAFGLVCSALSFLLLAASPSRSMPFVIASTI
                     LGALGGSIAGPLSAALVANSVADADRAKVMSLFNALVIAACSPFGYVGGLLSTVSERL
                     PFVLGTATFVPTIALAIVLPVIQRRYARKVESAAA"
     gene            complement(1317..>2302)
                     /locus_tag="VFH83_06755"
     CDS             complement(1317..>2302)
                     /locus_tag="VFH83_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6486100.1"
                     /translation="LCERLGVSRITVRSALLSLAQQGILSRRSGSGTFVNWDLSRTGT
                     IVFFRCEHGTGSHEMRNDLVYSEVLEGIQSEACTGGVHLIFSYVREEGDESQRVLKGL
                     SAKADGFIFGEMRNESFYREVASAYTPIVLINPEFSSYSGDSVDYDNLAGAIGATEHL
                     VELGHRYIGFINGRMPTRHAAERLDGYRIALARHGLPFDPTLVAGGVNWQSETGCEAV
                     KELVERNPEITAVFAANDALAVGAIQGLHDQGLDVPMDVSVIGFDDMVIASYSEPKLT
                     TMRVDRQEMGKAAARRLLELLRSPSEQAVKTLFVPKLIVRESCIAPDAPAD"
BASE COUNT          335 a          812 c          707 g          448 t
ORIGIN      
        1 ctgacccgag cgaccctctc ggcaagccat gctctcattc ctgcgcgctc agctccgctc
       61 cacccgtgag gtcatcgccg gcttcaaggg aaacgcccgc gcgctcctga tctgcgaacc
      121 gctctggggc attccctaca acctctacgc cacctatgcg tcggtgtaca tgctggccct
      181 ggggtgcacg gagacgcagg tcgggctgat cgccagctgc gccctggtgt cggcaatcgt
      241 cttctccctg gtgggcggct ggatcatcga ccggatgggc cgcaagcgcg ccaccctgtt
      301 cttcgacatc ctctcctgga gcatccccgg cgtgttgctg gcaacctccc gagggttcac
      361 catgttcctg ctggccgcgc tcatcaactc cctggtgcgc gtggtcaaca tctcctggga
      421 ctgtctgatg gtggaggaca cgccgccggc gcagcgggtg cacttctaca cctggatcgc
      481 ggtggccggc atcctggccg ggttcatttc tcccgtgtcc gggccggtgg tggaccgatt
      541 cggcctggtg cccgccatgc gggccctgta tgcctttgcc gcgatctcca tgaccgtcat
      601 gttcttctgg cgcaactcga tgacccacga gaccgagatc ggggccatga agatgcggga
      661 gacccgccac ggggatgccg ggaccacggc ctccgagtac gcgcggatca ccggccagct
      721 cctccgcaat cccctgaccc tgaccgcgct ggtcctgaac ctgcttttga ccatccagat
      781 gacgctcaag ggcacgttcc tgtccatcct gctcatccgg cggctggggt tctccgccgg
      841 aagcatttcc atctttcccg cgatcaactc cgcggtggtg ctgctggtct actccctggc
      901 catgcccgcg gtgggtcgga tgaagatcca ccggcctctg gccttcggcc tggtgtgctc
      961 cgctctcagc ttcctccttc tggcggcgag ccccagccgc agcatgccgt tcgtgatcgc
     1021 ttccaccatt ctgggcgcgc tgggcggctc cattgccggg ccgctctccg cggcgctggt
     1081 tgccaactcc gtagcggatg cggaccgtgc caaggtgatg tccctcttca acgcgctcgt
     1141 gatcgcggcc tgctcgccct tcggctacgt cggcggcctg ctctccacgg tatccgagcg
     1201 gctcccgttc gtcctgggca cagcgacctt tgttcccacg atcgccctgg cgatcgttct
     1261 gccggtgatc cagcgacggt acgctcggaa ggtcgagagc gcggcggcgt gaccgtctag
     1321 tcggcgggcg cgtccggggc gatgcaggac tctcggacga tcagcttggg aacgaagagc
     1381 gtcttgaccg cctgctcgct cggggaccga aggagctcga gcagcctccg tgcggcggcc
     1441 tttcccatct cctgccggtc cactcgcatc gtcgtgagct tcggctcaga gtaggatgcg
     1501 atcaccatgt catcgaaccc gatgacgctt acgtccatcg gaacgtcgag cccctggtcg
     1561 tgcagaccct ggatggcgcc gaccgccaga gcgtcgttcg cggcaaatac cgcggtgatc
     1621 tcgggattcc gttccaccag ctccttcacg gcctcgcacc cggtctcgct ttgccaattg
     1681 accccgcccg ccacaagggt cggatcgaac ggcagcccgt gccgtgccag cgcgattctg
     1741 tacccgtcca ggcgctccgc ggcgtgtcgg gtcggcatcc ttccgttgat gaacccgatg
     1801 taccggtgtc cgagctctac caggtgctcg gtcgcgccga tggccccggc caggttgtca
     1861 tagtccaccg agtcccccga gtagctggag aactccgggt tgatcaacac gatgggcgta
     1921 taggcgctcg ccacctcccg gtagaacgat tcgttccgca tctccccgaa aatgaagccg
     1981 tccgccttgg ccgagaggcc cttgaggacc cgctggctct cgtccccttc ctcgcggacg
     2041 taggagaaga tgaggtgcac gccgccggtg cacgcctcgg actggatgcc ctcgagcacc
     2101 tccgaataga cgaggtcgtt ccgcatctcg tggctgccgg taccatgctc gcagcggaag
     2161 aatacgatgg tccccgtgcg agacagatcc cagttgacga acgtgccgct ccccgagcgc
     2221 cggctcaaga tgccctgctg ggcgagggag aggagggccg accgcacggt gatgcggctc
     2281 acgcccaggc gctcgcacag ct
//
LOCUS       DASRZS010000274         3348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1308881, whole genome shotgun sequence.
ACCESSION   DASRZS010000274 DASRZS010000000
VERSION     DASRZS010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308881"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VFH83_06760"
     CDS             <1..712
                     /locus_tag="VFH83_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6486101.1"
                     /translation="TAAARLTTSHEPIYYGWKEEAAMLVKERMTRNPIFIRPETPVTE
                     AQALMKREKIHHLPVLDKEEKLVGIVTEKDLLYASPSVATTLSVYEMTSLLAKLKVDK
                     IMTREVVTVNEDVPLEEAARIMADRRIGGLPIVRGRALVGIITESDLFRIFIELLGAR
                     RPGVRVSVTVPNEKGELARLASAVTSAGGNIIALGTLPGEDPATGQCTLKVDGIAREA
                     LISALTPVVRSVDDIRET"
     gene            712..1938
                     /locus_tag="VFH83_06765"
     CDS             712..1938
                     /locus_tag="VFH83_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET6486102.1"
                     /translation="MEQKRLTLSQRGEEVPLSPIRKLTPLADAAKKRGIEIHHLNIGQ
                     PDIQTPQPIIDAYRGFDAKVLAYSPSLGIPSLRESISGYYRRYGLPVQPEDVAVTVGG
                     SEAIFFALSAVLDPGDEILVREPFYANYIAFAAMISAKVVPIPASFDNGFALPSREEI
                     ARRITPRTKAILYISPENPTGVVYDQQEMDTLRELAERFGLYLIADEVYREFIYDDHV
                     RFSSVLDLKGFEQQAILIDSISKRFSSCGARIGCIVSFNRGVMELVDKFAQARLCPPT
                     VGQLAADAAYRMDPSYFLPIRDEYRKRRDVLVEGLRRIPGVDLHVPEGAFYLMARLPV
                     EDAEDFCSFLLRDFEAGGKTVMLTPGAGFYSTPEKGRDEVRIAYVLEAPKIVESTAIL
                     AQALKRYGTRRKVQRA"
     gene            1950..3305
                     /locus_tag="VFH83_06770"
     CDS             1950..3305
                     /locus_tag="VFH83_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA2 domain-containing protein"
                     /protein_id="HET6486103.1"
                     /translation="MAKEAKPLIVIGHKNPDTDSICAAIAYARLSSEVLKEPASPYRA
                     GNVNLQTQFVLKRFEVESPPLATDVRSRIDDIMIPKSQLITLHERETLERACDLLTNH
                     HFSFLPVVDAAGRSLGKVTALRLVGVLRKLAAACREEGEPLGERERNVLAGTIGDLVD
                     RQSLSFKPSDVVKDVQREIGKSNEGGFLIQDADGMLKGVITRINFLEDSRLRVVMVDH
                     NEVAQAVDGIEEADVVQVIDHHRLGARTTTTPITFINRVVGSTCTIISDLYRNNGAVP
                     PKKDAGLMLSALLSDTLVLRSPTTTGLDRDVARWLGGLAGVDVERYGTEMIAAGSALE
                     GVDPRKIITQDQKVYTEAGRKFSLSQVETVSFDPVLALKEPLAAELEKLIQAEGLSFA
                     CLMITDLTRETSLLLCRGEARVLGAIEYPRREEKLFEMKDVLSRKKQLLPYFANLLKA
                     I"
BASE COUNT          598 a         1123 c         1091 g          536 t
ORIGIN      
        1 caccgccgct gctcggttga ccacgtcgca tgagcccatc tactatggat ggaaggagga
       61 ggccgccatg ctggtgaagg aacgcatgac ccgcaacccc atcttcatcc ggccggagac
      121 gcccgttacc gaggctcagg ccctgatgaa gcgggagaag atccaccatc ttcccgtcct
      181 ggacaaggag gagaagctcg tcgggatcgt gacggaaaag gacctcctct atgcctcccc
      241 gtcggttgcc accacccttt ccgtttacga aatgacctcg ctgctggcca agctcaaggt
      301 ggacaagatc atgacccgcg aggtggtgac ggtgaacgag gacgttccgc tggaggaggc
      361 ggcccggatc atggcggacc gccggatcgg cgggctgccc atcgtgcggg gccgcgccct
      421 ggtggggatc atcacggaga gcgacctctt ccgtatcttt atcgagctgc tcggcgccag
      481 gcgcccgggc gtgcgggtgt ccgtcacggt tcccaacgag aagggcgagc tcgcccggct
      541 cgcctccgcc gtgacctccg cgggcggcaa catcatcgcc ctggggacgc tcccgggaga
      601 ggaccccgcc acgggccagt gcacgctcaa ggtggacggc attgcccggg aggcgctgat
      661 cagcgccctc acgccggtcg tccgcagcgt cgatgatata agagagacct gatggagcaa
      721 aagagactga ccctttccca gcgcggggag gaggtacccc tctctcccat ccgcaagctt
      781 acgcctctgg cggacgcggc gaaaaagcgc ggcatcgaga tccaccacct gaacatcggg
      841 cagccggaca tccagacgcc ccagcccatc atcgacgcct atcgcgggtt cgacgccaag
      901 gtgctggcct acagcccgtc cctcgggatc ccgtccctgc gggagtcgat ctcgggctac
      961 taccgccgct atggcctccc cgtgcagccc gaggacgtgg ccgtcaccgt tggggggagc
     1021 gaggccatct tcttcgccct ctccgcggtg ctggaccccg gggatgagat cctggtgaga
     1081 gagccgttct acgcgaacta catcgcgttc gccgccatga tctccgccaa ggtggtgccc
     1141 atccccgcct ccttcgacaa cgggttcgcg ctcccctccc gggaggagat cgctcgacgg
     1201 atcacgcctc gcaccaaggc catcctgtac atctctccgg agaaccccac cggggtggtc
     1261 tacgaccagc aggagatgga taccctccgg gagctggcgg agcgcttcgg gctgtacctc
     1321 atcgccgatg aggtctaccg cgagttcatc tatgacgacc acgtgcggtt ctcctccgtg
     1381 ctggacctca aggggttcga gcagcaggcc atcctgatcg actccatctc caaacgcttc
     1441 tcctcctgcg gggcgcgcat cgggtgcatc gtctccttca accggggcgt aatggagctg
     1501 gtggacaagt tcgcccaggc gcggctgtgc ccgcccacgg tggggcagct cgccgcggac
     1561 gccgcctatc gcatggaccc ttcgtacttc ctgcccatcc gcgacgagta ccgcaagcga
     1621 cgcgacgtcc tggtggaggg gctgcggagg atcccgggcg tggacctcca tgtccccgaa
     1681 ggcgccttct acctgatggc gcgtcttccc gtggaagacg cggaggactt ctgcagcttc
     1741 ctgctgcggg acttcgaggc gggcggcaag accgtaatgc tcactccggg ggccggcttc
     1801 tactccacgc cggaaaaggg ccgcgacgag gtccgaatcg cctacgtcct ggaagccccc
     1861 aagatcgttg agtccacggc catcctggcc caggcgctca agcggtacgg gactcgtcga
     1921 aaggtccaac gggcgtaggg ggacaaccca tggcgaaaga ggccaagccg ctcatcgtca
     1981 tcggccacaa gaacccggac acggactcca tctgcgcggc catcgcctac gcgaggctgt
     2041 cttccgaggt gctgaaggag ccggcgtcgc cctatcgggc gggcaacgta aacctgcaga
     2101 cccagttcgt cctgaagcgc ttcgaggtcg agagcccccc tctggccacg gacgtccgct
     2161 cgcggatcga cgacatcatg atcccgaagt cccagctcat aacgctgcat gagcgcgaaa
     2221 cgctggaacg ggcctgtgac cttctcacga accaccactt ctccttcctg ccggtggtgg
     2281 atgcggccgg caggtccctg gggaaggtca cggcgctgcg gctggtcggt gtgcttcgga
     2341 agctggcggc cgcgtgtcgg gaagagggag agcccctggg ggagcgcgag cgcaacgtcc
     2401 tggccgggac catcggggac ctggtggacc gccagtccct cagcttcaag cccagcgacg
     2461 tcgtgaagga cgtgcagcgg gagatcggca agagcaacga aggcgggttc ctgatccagg
     2521 acgccgatgg catgctgaag ggcgtcatca cccggatcaa cttcctggag gacagccggc
     2581 tgcgggtggt catggtggac cacaacgagg tggcgcaggc ggtggacggc atcgaggaag
     2641 cggacgtggt gcaggtcatc gaccatcacc gcctgggagc gcgcaccacc accaccccca
     2701 tcacgttcat caaccgcgtg gtgggctcaa cgtgcaccat catctccgac ctctaccgca
     2761 acaacggggc ggtcccgccg aagaaggatg ccgggctgat gctctcagcg cttctctcgg
     2821 acaccctggt ccttcgctcc ccgaccacga ccggcctgga ccgcgatgtg gcgcgctggc
     2881 tggggggcct cgccggggtg gacgtggaac gctacggaac cgagatgatc gccgccgggt
     2941 cggcgctgga gggcgtggac ccccgcaaga tcatcaccca ggaccagaag gtgtacaccg
     3001 aggccgggcg caagttcagc ctgagccagg tggagaccgt cagcttcgat ccggttctcg
     3061 ccctgaagga gccgctcgcc gcggagctgg agaagctcat ccaggcggaa gggctctcgt
     3121 tcgcctgcct catgatcacg gatctgaccc gggagacgag cctgctcctc tgccgaggag
     3181 aggccagggt cctgggggcc atcgagtacc cgcggcggga agagaagctc ttcgagatga
     3241 aggatgtcct ctcccgcaag aagcagcttc tgccctactt cgccaacctc ctcaaggcga
     3301 tctgagtcga gccggaccgg cgggtccagc cgtgtcccga gggggccg
//
LOCUS       DASRZS010000275         3840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3232986, whole genome shotgun sequence.
ACCESSION   DASRZS010000275 DASRZS010000000
VERSION     DASRZS010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3232986"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VFH83_06775"
     CDS             complement(<1..1024)
                     /locus_tag="VFH83_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486104.1"
                     /translation="MPSEEVVRPEAILSRALTAFADLDAKDGGPQADGGPKADARGCT
                     DARAWVENHVPLLDCPDAAIQTSYYFRWWTYRKHIRKTDLGYIVTEFLPEVPWAGYAN
                     SIVAAAGHHLYEGRWLRGSRDFLADYVSFWFRKEADSFRYRTWLADAVRAYACVTGDL
                     ETVRQVLPALIDRQRKLQGLHAHSSGLYWSDDDHDAMEYSISGNGLRPTLNSYLFGDA
                     RAIAEMASWLGEAGIARDLEAEAERLRALVLRLWQKPASFFKTVHAETQDSDVGLLPD
                     AVAKAHDARELIGFIPWYFGLPPDEEEYGAAWSQLLDEDGFQAPFGLATAERRHPRFM
                     FKADHEC"
     gene            complement(1029..1934)
                     /locus_tag="VFH83_06780"
     CDS             complement(1029..1934)
                     /locus_tag="VFH83_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486105.1"
                     /translation="MTRGASNRWIKHQGNPVLGGEYGTCFDLSMLRLDGRYRMYFSWR
                     PRKAIALTESADGFTWSKPTIVLQGSGGDSWDSDVNRPSVILREGRFEMWYTGQDTSG
                     TERGSRIGYATSPDGLSWKQREQPILEACGGWEGGNLMCPHVLYDDAESIYKMWYSGG
                     ERYEPDSIGYATSTDGVAWKRWAANPVFVPDSTHPWEAYKVTAGQVIKTKSGYLIFYI
                     GFQDIDTARIGIARSSTGVDGWRRSRANPIISPDPGAWDGEACYKPFAMRAEHGWMLW
                     YNGRSGHLEQIGAATLEGDELGFDD"
     gene            1963..2871
                     /locus_tag="VFH83_06785"
     CDS             1963..2871
                     /locus_tag="VFH83_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET6486106.1"
                     /translation="MDYSACGFQTGMDENRENMDKNALPGRWREPARNSETEAFLLGL
                     ANQEIRHIGTWRIVGDSGWRLQTHSHAFYEFLYFVRGKTKVHLDRSGFTIHPCNLVIY
                     PPGVTHHEYPNFHGDRHDVICAHFDIGANPQGTGPIVANDPHGHLIWLFERLHDEAGA
                     DGHDISRDLLRVILRHTAKVVAASGGRGGSIIGLATLYLQNYIDQSINVERIARAART
                     SPAYLGRTFARVLGTSPTHFAVLMKMETAKRKLSLTDDSVAEIGRTVGYEDPFYFSRL
                     FRKVTGMSPREFRERARVETTPSSAR"
     gene            complement(2914..3678)
                     /locus_tag="VFH83_06790"
     CDS             complement(2914..3678)
                     /locus_tag="VFH83_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6486107.1"
                     /translation="MMPILVSNPNPFGGTVIDPAALPRDPEEFRASLAFSLASWREER
                     IKVAWLEVPVDLSSLIPVAVAAGFSFHHAVEGYAMLTARLVEGAFIPPYATHYVGAGG
                     VVVNERQELLVVCERHRRSPQPSYKLPGGALHPGEDLQVCVQREIVEETGIRTTFEAL
                     IGFRHWHGYRYGKSDIYCICRLAPLDQEIRMQAEEIEECLWMPLTEFLASPFVHVFNR
                     LVVRAALASPGIRPTRIEGYSDATREIFLPPDVPTD"
BASE COUNT          701 a         1247 c         1218 g          674 t
ORIGIN      
        1 ggcactcgtg atcagccttg aacatgaagc gtgggtgccg gcgttcggcg gtggcgaggc
       61 cgaagggagc ctggaagccg tcctcatcca ggagctggct ccacgcggcg ccatattcct
      121 cctcatcggg agggagaccg aagtaccacg ggatgaagcc gatcagctcg cgagcgtcgt
      181 gggccttcgc cacggcgtcg gggagaagtc cgacgtccga atcctgcgtt tcggcgtgga
      241 ctgtcttgaa gaaagacgcc ggcttctgcc agaggcgcag aacgagcgcc cggagtcttt
      301 ccgcttccgc ttccagatcc cgggctatgc ctgcctctcc cagccacgat gccatttcgg
      361 cgattgccct cgcatcgccg aagaggtagg aattgagcgt gggccgcagc ccgttgccgc
      421 tgatggaata ctccatggca tcatggtcat cgtccgacca gtacagcccg ctggagtgag
      481 cgtggagacc ctggagcttc cgttgccggt cgatgagggc aggcagaacc tggcgcacgg
      541 tctccaggtc ccccgtgacg caggcatacg cgcgcacggc gtccgcaagc cacgtccggt
      601 aacggaatga gtctgcttcc tttcggaacc agaacgagac gtagtccgca aggaaatctc
      661 gtgagcctcg cagccagcgc ccttcgtaga ggtggtgccc cgcggccgcg acgatggagt
      721 ttgcgtaccc ggcccaggga acctcgggga ggaactccgt gacgatgtac ccgaggtccg
      781 tcttcctgat gtgcttgcgg tacgtccacc agcggaagta gtagctggtc tggatcgcgg
      841 catcgggaca gtcgagcagg ggcacgtggt tctcgaccca cgcgcgggca tctgtgcacc
      901 cgcgggcgtc ggccttcgga ccgccgtcgg cctgcgggcc tccatccttc gcgtccaggt
      961 ccgcgaatgc cgtcagggca cgggagagga tggcctcggg ccgaaccacc tcctcggacg
     1021 gcatcgtttc agtcgtcgaa cccaagctca tcgccttcca gcgtggctgc cccgatctgc
     1081 tccaggtgcc ccgatctgcc gttgtaccag agcatccagc cgtgctcagc ccgcatcgcg
     1141 aaaggcttgt agcaggcttc cccgtcccag gccccggggt ccggggagat gatggggttt
     1201 gccctggagc gtcgccaacc gtcgaccccg gttgaggacc tggcaatccc gatgcgggcc
     1261 gtgtcgatgt cctggaatcc gatgtagaag atgaggtagc cgctcttcgt cttgatgacc
     1321 tgcccggccg tcaccttgta ggcctcccaa gggtgggtgg agtcagggac aaagaccggg
     1381 tttgccgccc atcgcttcca cgcaacgcca tccgtgctcg tggcgtatcc gatggagtcg
     1441 ggctcgtagc gttcgccgcc cgagtaccac atcttgtaga tcgactccgc atcgtcatag
     1501 aggacgtggg ggcacatcag gttcccgcct tcccaccctc cacaggcctc cagaatcggt
     1561 tgctcgcgtt gcttccagga gaggccatcg gggctggtcg cgtagccgat tctcgagccg
     1621 cgttcggtcc cgctggtgtc ctgccccgtg taccacatct caaagcgccc ttcccgcagg
     1681 atgaccgatg gcctgttgac atcgctgtcc caggagtcgc ccccggagcc ctgcagcacg
     1741 atggtgggtt tggaccacgt gaatccgtcg gcgctctccg tcagggcgat ggccttgcgc
     1801 ggccgccagg agaaatacat ccggtacctt ccgtccagcc tcagcatgga gagatcgaaa
     1861 caggtgccgt actccccgcc cagtacggga ttgccctggt gcttgatcca tcggttgctt
     1921 gcgccgcgcg tcatttcagg ctccctttcg aggaaatcgg gtttggatta tagtgcctgc
     1981 ggattccaga cgggaatgga cgagaaccgc gaaaacatgg acaagaacgc actccctggg
     2041 cggtggcggg agcccgcgcg aaactccgaa acggaggcat ttctcctggg gctggccaac
     2101 caggagatcc ggcacatagg aacctggagg atcgtggggg actccgggtg gcggctgcag
     2161 acgcactctc atgcgttcta tgagttcctc tatttcgtgc gggggaagac aaaagtgcac
     2221 ctggaccgat ccgggttcac gatccatccc tgcaacctgg tcatctaccc gcccggggtc
     2281 acgcaccacg agtacccgaa ctttcacgga gatcgccatg acgtcatctg tgcccacttc
     2341 gacattgggg cgaacccgca aggaacgggc cccatcgtgg ccaacgatcc gcatgggcac
     2401 ctcatctggc tctttgaacg gctgcatgac gaagcggggg cggatgggca tgacatttcc
     2461 cgagaccttc tgcgcgtcat cctcaggcac acggcgaagg tcgtcgctgc ctcgggtggc
     2521 agaggcggca gcatcatcgg ccttgccacc ctctacctcc agaactacat agaccaatcg
     2581 atcaatgtcg agcggatcgc tcgggcggcg cgcaccagcc ccgcatacct cggcaggacc
     2641 ttcgccaggg tgctgggaac aagccccacg catttcgcgg ttctcatgaa gatggagacc
     2701 gccaagcgga agctatccct taccgacgac tccgtggcgg agatcggtcg caccgtgggt
     2761 tacgaggatc ccttctattt ctcgaggctc ttccgcaagg tcaccggcat gagcccccgg
     2821 gagtttcgag aaagagccag ggtcgagact acgccgtcga gcgcccgctg agcgcgagta
     2881 aggggcaggg agcggcgagc tcaggagatg cgctcaatcg gtcggcacgt ccggcgggag
     2941 gaaaatctcg cgcgtcgcat ccgaataccc ctcgatgcgg gtaggacgga tcccggggct
     3001 ggccagcgcc gccctcacga ccaggcggtt gaagacgtgg acgaaggggg acgcaaggaa
     3061 ctccgtcagc ggcatccaga ggcactcttc gatctcctcg gcctgcatcc ggatctcctg
     3121 gtcgagcggc gcaaggcgac agatacaata gatgtcggat ttcccgtagc gatatccgtg
     3181 ccagtgccgg aagccgatca acgcttcgaa cgtcgtgcga atcccggtct cctcaacgat
     3241 ctcccgttgg acgcagacct gcaggtcctc gccaggatgg agagcaccgc ccgggagctt
     3301 gtaggaaggc tgcggcgagc gccgatgccg ctcgcagacg accagcagct cctgtcgctc
     3361 gttgaccact accccgccgg cgcccacgta gtgcgttgca tagggcggga tgaaggcgcc
     3421 ttccaccagc cgcgcggtga gcatagcgta gccttccacg gcgtgatgga agctgaaccc
     3481 ggccgccacg gcgaccggga tcaacgacga cagatcgacc gggacctcca gccatgccac
     3541 cttgatccgc tcttcccgcc acgaagcgag ggagaaagcg agactcgctc gaaactcctc
     3601 aggatcacgc ggcagagcgg cggggtcgat cacggtgccg ccgaacggat tggggttact
     3661 gaccaggatc ggcatcatgc aaccttcttg gggagagggc cggccgagga ctcgccggca
     3721 cccccctgtg gggtcaaccg gcgtatccgt cgtcgatctc gcgatcgagc gccccgctct
     3781 cctcggtgaa gcccacgacg catgctacgc ggcacaccct gcggccgtca acccgaacgc
//
LOCUS       DASRZS010000276         3176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4002015, whole genome shotgun sequence.
ACCESSION   DASRZS010000276 DASRZS010000000
VERSION     DASRZS010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4002015"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFH83_06795"
     CDS             <1..573
                     /locus_tag="VFH83_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6486108.1"
                     /translation="VTPDDVEAGAHAGSYLVANGHRRIAFVGIKGHTPSELRLKGFLR
                     SAAQSAVAVPPDLLRWCEGADSQWRSGAHAATAEMLSLGAARPTAVFYFNDEAASQGY
                     AAIREAGLSVPGDISVVGFDDSQISRALYPSLTTFLHPKDRIGEMAARILLDRIEDPD
                     SSLPVRVTYTCKLIERDSVRNLAKEKHLNG"
     gene            566..2971
                     /locus_tag="VFH83_06800"
     CDS             566..2971
                     /locus_tag="VFH83_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486109.1"
                     /translation="MADPYKEDVEPAQRRVEAWWHGQIVDRAVVQAAAPRPGAVAQTA
                     DAGDSRSQEQLQSYFMDPEIVIPRLTLRLRRTWFGGEAFPVMFPVSIGMVAILANYLG
                     CPLRFVGTETTWHDPVIQDWDHAPSFQYDPDHPLWRGSRALLQAAVDRSDGYFVGCPD
                     LNGPTQALGLMRDNERLALDFYDHPDRIKPALAKINQAWYRYWRECAAITQGTGGNFY
                     WMGFWSEKTSIDLQSDFSCMLSVEQFNEYFLPFIEEQTRLADRTMYHLDGPGAIRHAD
                     ALLALPRLTGIQWIQGAGGGPVLQYLPLLKKIQDAGKLVSFFCEKKELERIFEELSPE
                     GMFPIVTDCESPYESQEVVRRVERLSARGHARPARHGGNGRGQIPPAEGDVLRGLAER
                     KARIASTALNQGRREDWYRHNALQGTRPMILAEIQGVMDETVPDSALVCAHPVARRLE
                     RSLRHDLYLHEAVRDDFVMEPWIDIPWKVAATPYVPESELSIHVPKRDDGKIGARRWD
                     PPIRDISRDFGKLRKREFSVDREGTLAEKADLEAVFEGILPVRIRGAYWWTFGMTWTI
                     IDLIGLESLMLFMYDDPEGLHRLMRFVHDDHVDFAAWLEENGLLNLNNENDYIGSGSR
                     GHVRELPGPGLPPQQAGSPSRPARSKDLWLLLESQETVGVGPEQFEQFVFPYQLDLAV
                     RFGLVYYGCCEPVHSRWKVIQKIPNLRSVSVSPWADEAFMAEALHGKYVYSRKPNPAH
                     VSTSIFDETAIRADIRHTFEVARGCDIEIVMKDVHTLNGHPERLGRWVQIAREESDRV
                     FGS"
     gene            complement(2980..>3176)
                     /locus_tag="VFH83_06805"
     CDS             complement(2980..>3176)
                     /locus_tag="VFH83_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET6486110.1"
                     /translation="LWGGGVNTQRTLPLGSVAEVEREVSEVVPVMAKDGGFVFCAIHN
                     ILAEIDPAKVVAMYRAASRT"
BASE COUNT          552 a         1047 c         1058 g          519 t
ORIGIN      
        1 gtgaccccgg acgacgtgga ggcaggcgcc cacgccggct cgtacctggt ggccaacggg
       61 caccggcgga tcgcattcgt cggcatcaag ggacacaccc caagcgagct tcggctgaag
      121 ggattccttc gctccgcggc gcagtccgct gtggcggtcc ctccggatct ccttcgctgg
      181 tgcgagggcg cagactccca gtggcggtcc ggcgcccatg ccgccaccgc cgagatgctt
      241 tccctggggg ccgcccggcc gacggccgtg ttctacttca acgacgaggc ggcatctcag
      301 ggctacgccg cgatccggga ggccggtctc tccgtgccgg gggatatctc tgtggtgggt
      361 ttcgatgact cgcagatcag ccgcgccctg tatccgtccc tgacaacgtt cctccatccg
      421 aaggatcgga tcggagagat ggccgcgcgg atcctgttgg atcggatcga ggacccggat
      481 tcctccctgc ccgtacgggt gacctatacc tgcaagctga tcgagcggga ttcggttcga
      541 aacctcgcaa aggagaaaca cctgaatggc tgatccgtac aaggaagacg tcgagccggc
      601 gcagaggcgc gtcgaagcgt ggtggcacgg acagattgtc gatcgggcgg tcgttcaggc
      661 cgcggcaccc cggccgggag ccgtcgctca gaccgccgac gcgggagact cccgaagcca
      721 ggagcagctc cagagctact tcatggaccc ggagatcgtg atcccgcggc tcacgcttcg
      781 gctgcgccgc acctggttcg ggggagaggc ctttccggtg atgttcccgg tgtccatcgg
      841 tatggtggcc atcctggcga actacctggg ctgccccctg cggtttgttg gcacggagac
      901 cacgtggcac gaccccgtga tccaggactg ggatcacgcc ccgtcgttcc agtacgaccc
      961 ggaccacccg ctgtggcgcg gctcccgcgc gctcctgcag gcggccgtcg accggtccga
     1021 cggctacttc gtaggctgcc cggacctcaa cggtccgacc caggccctgg ggttgatgcg
     1081 ggacaacgag cgcctggccc tggacttcta cgaccacccg gaccggatca agccggccct
     1141 cgcgaagatc aaccaggcat ggtatcgcta ctggcgggaa tgcgcggcca taacgcaagg
     1201 caccggggga aacttctact ggatgggctt ctggtcggag aagacgtcca tcgacctcca
     1261 gtcggacttc tcctgcatgc tgtccgtgga gcagttcaac gagtacttcc tcccgttcat
     1321 tgaggagcag acccgtctgg cggaccgcac gatgtaccac ctggacggac ccggcgccat
     1381 tcgccacgcg gacgctctcc tggcgctccc gcggctcacg gggatccagt ggatccaggg
     1441 agccggcggg ggtcccgtgc tccaatacct gccgctgctc aagaaaatcc aggatgccgg
     1501 gaagctcgtg tccttcttct gcgagaagaa ggagctcgag cgcatcttcg aggagctgtc
     1561 accggagggg atgttcccca tcgttaccga ctgcgaaagc ccgtacgagt ctcaggaggt
     1621 ggttcgacgg gtggagcggt tgagcgcgcg gggccacgcg cgcccagcac gccacggcgg
     1681 gaacgggcga ggtcagatcc cgccggcgga gggggacgtg ctccgcgggc tggcggagcg
     1741 caaggcccgc atcgcatcca cggctctgaa ccaggggcgc cgcgaggact ggtaccggca
     1801 caacgccttg cagggcacgc gacccatgat cctcgcggag atccaggggg tcatggacga
     1861 gacggtgccg gactccgccc tggtgtgcgc gcacccggtg gcacggcgcc tggagcgcag
     1921 cctgcggcat gacctgtacc tgcacgaggc ggtgcgggac gacttcgtga tggagccctg
     1981 gatcgacatt ccgtggaagg tggcggcgac accctacgtg ccggaatcgg agctctcgat
     2041 ccacgtcccc aagcgggatg acgggaagat cggggcccga cggtgggacc cacccattcg
     2101 agacatcagc cgggacttcg gcaagctccg caagcgggag ttcagcgtgg accgggaggg
     2161 cacgctcgcg gagaaggcgg acctggaggc ggtcttcgaa gggatcctgc cggttcgcat
     2221 ccgaggcgcc tactggtgga cgttcggcat gacctggacg atcatcgacc tgatcggcct
     2281 ggaaagcctc atgctcttca tgtacgacga ccccgaagga ctgcatcgcc tcatgaggtt
     2341 cgtccacgat gaccacgtgg atttcgccgc gtggctggaa gagaacgggc ttctgaacct
     2401 gaacaacgag aacgactaca tcggatccgg cagccgcggc cacgtccggg agctgcccgg
     2461 acctgggctc ccgccccagc aggccggctc acccagccga cccgccaggt caaaggacct
     2521 gtggctgctg ctcgaatccc aggagaccgt cggcgtcggc cccgagcagt tcgagcaatt
     2581 cgtcttcccc taccagctcg atctggccgt tcgattcggt ctggtctact acggctgctg
     2641 tgagccggtc cacagccggt ggaaggtgat ccagaagatc cccaatctgc gaagcgtctc
     2701 ggtttccccg tgggcggacg aggcattcat ggcggaggcc ctccacggta agtacgtcta
     2761 ctcccgcaag ccgaaccccg cgcacgtgag cacgtcgatc ttcgatgaga cggcgatccg
     2821 cgcggacatc cgacacacgt tcgaagtggc ccgtggctgt gacatcgaga tcgtcatgaa
     2881 agacgtccac accctgaatg gccacccgga gcgcctggga cggtgggtgc agatcgcgcg
     2941 agaggagtcg gaccgagtct tcggctccta gcccgtgcgc tacgtccggg aggcggcccg
     3001 atacatggca acgaccttgg cggggtcgat ttccgccagg atgttgtgga tggcgcagaa
     3061 tacgaagccg ccgtccttcg ccatcaccgg tacgacctcc gagacctctc gctcgacctc
     3121 cgcgacgctg ccaagcggca gcgtgcgttg cgtgttcacg ccgccgcccc acagcg
//
LOCUS       DASRZS010000277         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1692485, whole genome shotgun sequence.
ACCESSION   DASRZS010000277 DASRZS010000000
VERSION     DASRZS010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692485"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFH83_06810"
     CDS             <1..556
                     /locus_tag="VFH83_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486111.1"
                     /translation="ADPRFHARRSARLRVYRYYTVSSPVLLPHLRNYRHWVRRSLDVD
                     RLNAMAGVLVGEHDFSAFAAEGDANQSRVRLVTTSSFETKGDTVVYTIAATSFLWKMV
                     RTILGTFLMLEEQGRGPSDLTTILLSGRRSEAGGTAPARGLFLERVFYDAEEPEHGRA
                     LESAESGGGLPFGGVLQGASGPGD"
     gene            471..1256
                     /locus_tag="VFH83_06815"
     CDS             471..1256
                     /locus_tag="VFH83_06815"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET6486112.1"
                     /translation="MAALWKALNLVEDYLSGGFSRAHPVLAIDESAPAAAGVAGTARP
                     AAEGLQPAPGGSDPVSPISPMSPIAPIAETPEARLALLQAIAAEVSGCARCGLSANRK
                     TTVPGEGSLDPPVLFIGEGPGADEDRTGRPFVGAAGQYLDTWLPPIGLERQRCFIANV
                     VKCRPPQNREPHPEEITACFPYLLRQVEALRPKVICCLGRIAAQRLLGTTQSLGELRG
                     RKHQWKGIPVVVTYHPSAVLRDKTSLRKPVWEDLKLLKSILTD"
     gene            1273..>3160
                     /gene="priA"
                     /locus_tag="VFH83_06820"
     CDS             1273..>3160
                     /gene="priA"
                     /locus_tag="VFH83_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET6486113.1"
                     /translation="MGGFVDVVFNLPVKRQFAYRLPEGCLAPVGTRVSAPFSSRTLTG
                     LVVAERDLPPDTVAEIREIKRVVDGRPLIDERLLELARWMSQLYLCSLGQAVFTMIPG
                     GKRESEPDEMPPEEDARGYRLAEQQLSAIRAISEGTADTSYLFGVTGSGKSDVYLHAA
                     QDVTAKGRGVIYLVPEITLTHQVVRLFRTQYGHRLAVLHSALSPSQRLSEWLRILDGG
                     VDVVIGARSAVFAPVPRLGLIVIDEEHEGSYKSGSTPRYHARQVAQHRVSVEGARLVM
                     GSATPSLEAYERMSRGKLLSLRMPDRLSGGRMPEVEIVDMRRHTGPLSAPLLDQIART
                     RAEGRQAILFLNRRGFSYLFHCRSCGYEMTCKHCSVSLTYHKERERMICHYCGFSARP
                     ISVCPQCGSLDVGYSGYGTEGIEEELARLFPQLVARRLDTDAVRKRKVLRQALTDFRD
                     GKIHVLIGTQMVAKGLDFPGVKLVGIVNADTGFQLPDFRAAERTFGLLVQVSGRAGRA
                     LPDGRVLIQTFRPTTPAIVMAQQGKLEEFYAAELETRRLLGFPPYSRLIRLVMRGRNQ
                     KKAADAIGALARALASRLGDAAEVLGPAECPLARISGNYRYQIILRTTRFTEAHARAS
                     AELDA"
BASE COUNT          492 a         1057 c         1071 g          540 t
ORIGIN      
        1 ggcggatcca cgcttccatg cccggcgctc ggcccggctg cgcgtgtacc ggtactacac
       61 cgtctccagt ccggttctcc tcccccatct gcgaaactat cgtcactggg ttcgcagaag
      121 cctggacgtc gaccgactca acgccatggc cggcgtcctg gtcggcgagc acgacttttc
      181 cgcgttcgcc gcggagggag acgccaacca atcccgggta cgccttgtga ctacctcgtc
      241 cttcgagaca aaaggggaca cggtggtgta caccatcgcc gcaacctcct tcctctggaa
      301 gatggtgcgg accatcctgg ggacgttcct catgctggag gagcaggggc ggggaccctc
      361 ggatctcacc accattctct tgtccggccg caggtcggag gcgggtggaa cggcgccggc
      421 ccgtgggctg ttcctggaaa gggtgttcta tgacgcagaa gagccggaac atggccgcgc
      481 tctggaaagc gctgaatctg gtggaggact acctttcggg ggggttctcc agggcgcatc
      541 cggtcctggc gattgacgag tccgcgccgg cagccgccgg cgttgccggg actgcccggc
      601 ctgccgcgga aggcttgcag ccggcgcctg gcggatctga tccggtctcc ccgatctctc
      661 cgatgtcccc gatcgccccg atcgccgaga cgccggaggc gcgtctggcc ctccttcagg
      721 caatcgcggc cgaggtctcc gggtgtgccc gctgcggcct ttccgccaat cgaaagacca
      781 cggtgccggg cgaaggctcg ctggacccac cggtgctctt catcggcgag ggccccggtg
      841 cggacgagga tcggaccggc agacccttcg tgggagccgc gggtcagtac ctggacacct
      901 ggctgccgcc catcggtctg gaacgacagc gttgcttcat cgccaacgtc gtgaaatgcc
      961 ggccacccca gaaccgcgag ccgcaccccg aggagatcac ggcctgtttc ccgtatctgc
     1021 ttcggcaagt ggaagcgctg cggccgaagg tgatctgctg cctggggcgc atcgcggccc
     1081 agaggctgct gggaacaacg cagagcctgg gagagctgcg gggccgcaag caccagtgga
     1141 agggcatccc ggtagtggtc acgtatcacc ccagcgccgt ccttcgggac aagacgtccc
     1201 tgcgaaaacc cgtctgggag gacttgaagc tcttgaagag catcctcacc gactgaaggg
     1261 tcctggcgcc ggatgggcgg tttcgtcgac gtcgtcttca atcttcccgt caagcgccag
     1321 ttcgcctacc ggctgccgga ggggtgcctg gcgccggtgg ggactcgcgt ctcggcgccc
     1381 ttctcgtccc gaacgcttac cggcctggtg gtggccgagc gggatctgcc gccggacacc
     1441 gtggccgaga tccgagagat caagcgggta gtggatggcc ggccgctgat cgacgaaagg
     1501 ctcctggagc tggcgcggtg gatgtctcag ctctacctct gctccctcgg ccaggccgtc
     1561 tttacgatga tcccgggagg gaagcgcgag agtgagccgg acgagatgcc gccggaggag
     1621 gacgcccggg gataccggct tgccgagcag cagctctccg ccattcgcgc gatttccgag
     1681 ggcacggcgg acaccagcta tctcttcggg gtgaccggct ccggcaagag cgatgtgtac
     1741 cttcacgcgg cccaggacgt cacggcgaag ggcaggggcg tcatctacct ggttcccgag
     1801 atcacgctga cccaccaggt ggtccgtctc ttccgaacgc agtacgggca tcgactggcc
     1861 gtgctgcact ccgcgctctc cccgtcccag cgcctgtcgg agtggctgcg catcctggac
     1921 ggtggggtgg acgtggtgat cggcgcccga agcgccgtgt ttgcgcccgt gccccggctg
     1981 ggcctcatcg tcatcgatga ggagcatgaa ggctcctaca agtccggctc cacgccgcgc
     2041 tatcacgcgc ggcaggtggc gcagcatcgc gtctccgtcg agggagcgcg cctggtcatg
     2101 ggaagcgcca ctccttccct ggaggcgtac gagcggatga gccgggggaa gctcctttcc
     2161 ctgcggatgc cggaccggct gagcggcgga cggatgccgg aggtggagat cgtcgacatg
     2221 cggcgccaca ccgggccgct ctcggcgccc ctgctggatc agatcgcgcg cacccgcgcc
     2281 gaaggtcggc aggccatcct gttcctgaac cgccgcgggt tctcgtatct cttccactgc
     2341 cgctcctgcg gttacgagat gacctgcaag cactgctccg tctcgctcac gtaccacaag
     2401 gagcgggagc ggatgatctg tcattactgt gggttctcgg cccgacccat ctcggtatgt
     2461 ccccaatgcg gttccctgga cgtcggctac tcgggctacg gaacggaggg catcgaagag
     2521 gagctggccc gtctcttccc tcagctcgtc gcaagacgcc tggacacgga cgcggtccga
     2581 aagaggaagg tgctccgtca ggccttgacc gatttccgtg atgggaagat ccacgttctg
     2641 atcgggacgc agatggtggc caagggactg gatttccccg gtgtgaagct ggtgggcatc
     2701 gtgaacgcgg acacggggtt ccagcttccg gatttccgcg ccgcggagcg caccttcggg
     2761 ctcctggtgc aggtctcagg gcgggccggc agggccctgc ccgacggacg ggtcctgatc
     2821 cagaccttcc gccccaccac gccggccatc gtcatggccc agcagggcaa gctggaggag
     2881 ttctacgcgg cggagctgga gacccgccgc ctgctcggat tcccgcccta cagtcggctc
     2941 atccggctgg tgatgcgtgg ccgcaaccag aagaaggcgg ccgacgccat cggcgcgctc
     3001 gcccgcgcgc ttgcctcgag gttgggggac gcggcggagg tcctggggcc ggcggagtgc
     3061 ccccttgcca ggatttccgg caactaccgc taccagatca tcttgcgcac cacgcggttc
     3121 accgaggccc acgcgcgcgc cagcgcggag ctggacgcct
//
LOCUS       DASRZS010000278         3618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2465017, whole genome shotgun sequence.
ACCESSION   DASRZS010000278 DASRZS010000000
VERSION     DASRZS010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2465017"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2099
                     /locus_tag="VFH83_06825"
     CDS             <1..2099
                     /locus_tag="VFH83_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HET6486114.1"
                     /translation="YENRESQVEMLKLVCRAFNGDELCAAEAGTGVGKSLAYLLPSLS
                     WALQNGERVVISTNTINLQQQLVEKDIPLVKRILDQDPKVVLVKGRGNYLCAHRLSEA
                     LEEMSLFEDRDPELLSIRDWARTTETGSRTDLSFYPSDEVWSRVCSEADTCLGLRCGH
                     RDGCFVLKARREASSAQVLVANHHLLFADLALRIAGSGFEDPAVLPPFRRIVFDEAHN
                     IEKAATSFFSQSFSRFAIARFLGRLYRRKRGKITGLFPQLARHLGRSELTQRIPSLIF
                     AALERGEEVDEACLALMGEEGSLLLDARTWPTFLNACSGALGNLSFAIQETVQAFSNA
                     IEAVEAAEKGPETENTLWDCRVQLTRLSGIVELCNRFRSGDPGRNDIFWLESVRRKRA
                     APEAESGSVPATATERSVRLIMTPLDIGPMMRDAVYEPIKSAVFTSATLTVGGSFSFW
                     SNRIGLDRNVGREPLARIFPSPFNYREQVLLGVPTDAPTPDSPPFAAYVARFVTEAVL
                     ASRGAALVLFTSYSLLRETHAAALATLAQAGIRILRQGEDDRARLLEAFRSKRSNVLF
                     ATDSFWEGVDAPGDTLELVVLCRLPFRVPSEPVLKARMIAIEARGGNPFSELSLPDAV
                     VRLRQGFGRLMRRHDDTGVVLVLDSRIVSRTYGAVFMDSLPVTRRSIAPSSEVLADVR
                     EFFSRRESEGEEGEGG"
     gene            complement(2129..2392)
                     /locus_tag="VFH83_06830"
     CDS             complement(2129..2392)
                     /locus_tag="VFH83_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET6486115.1"
                     /translation="MKVTPLGDRVLVKLVEMETKTAGGILIPQTAQEKTQEGVVLAVG
                     EDESIKVKPKDRVIYDKYAGTTIKVGGEEQLILKASDILAKVE"
     gene            2577..3257
                     /locus_tag="VFH83_06835"
     CDS             2577..3257
                     /locus_tag="VFH83_06835"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HET6486116.1"
                     /translation="MPAIRWDLQVKRGLWALSKHHPSEAVQLLQQALENCPPSRTQEL
                     HRICFYLGIALRRLGFSQSAIKSWISCQRLNKRGHTRKMLLRLTNGYGMERQDTVEGD
                     DWQAFYSIQAARYLLNKNKRTFSVEAEKDMIGDLIRDSWNQLQASEALTDKSSCEKLE
                     VFRSVVIVFPTTVAAEPKAGESVIAVNFRTRRKVALTDRCICGSGLPFVQCCGRTPGK
                     EELLSGIF"
     gene            3427..>3618
                     /locus_tag="VFH83_06840"
     CDS             3427..>3618
                     /locus_tag="VFH83_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HET6486117.1"
                     /translation="MATAVVVDRERKNTRTDENILSLYLKEINRIPLLTREEEERLAR
                     RAAKGEQLAKESLIRANLRF"
BASE COUNT          669 a         1135 c         1120 g          694 t
ORIGIN      
        1 tctatgagaa ccgcgagagc caggtggaaa tgctcaagct ggtatgccgc gcgttcaacg
       61 gagacgagct gtgcgcggcg gaggccggca ccggagttgg gaaatctctg gcgtacctcc
      121 tgccttcgct ctcctgggct ctccagaacg gggagcgcgt ggtgatctcc acgaacacca
      181 tcaacctgca gcagcagctc gtggaaaagg acattccgct ggtgaagcgc atccttgacc
      241 aggatcccaa ggtggtcctg gtcaagggca gggggaacta tctctgcgcc caccgcctct
      301 cggaagccct ggaggagatg agcctcttcg aggatcgcga tcccgagctt ctctccatcc
      361 gggattgggc caggaccacc gagacgggca gccgcacgga tctctcgttc tacccgagtg
      421 acgaggtgtg gtcccgggtc tgttccgaag cggacacgtg cctggggctg cggtgcggac
      481 accgcgacgg ctgtttcgtc ctgaaggcgc gtcgggaggc ttcctcggcc caggtgctgg
      541 ttgcgaacca tcacctcctc ttcgcggacc ttgccctgcg gatcgccgga tccggattcg
      601 aggaccccgc ggtcctgccc ccctttcgac ggatcgtgtt cgacgaagcc cacaacatcg
      661 aaaaggcggc cacgtccttc ttctcccaga gcttctcccg attcgccata gcccgattct
      721 tgggtcgcct gtaccgtcgc aagcgcggaa agatcacggg gctctttccc caactagccc
      781 gccatctcgg cagaagcgag cttacccagc ggataccgtc cctcatcttc gcggcgctgg
      841 agcgagggga ggaggtggat gaagcgtgcc tcgcgctgat gggcgaggaa ggctccctgc
      901 tcctggacgc acggacctgg ccgacctttc tcaacgcctg ctcaggggcg ctcgggaacc
      961 tctcctttgc gatccaggag acggttcagg ctttctccaa cgcaatcgag gccgtggagg
     1021 cagcagagaa gggtccggag acggagaaca cgctctggga ttgcagggtc cagctcactc
     1081 gcctgtcggg cattgtcgag ctgtgcaatc gctttcgaag cggtgaccct ggacgcaatg
     1141 acatcttctg gctcgagtcc gttcgacgga agcgcgcggc gccggaagcg gaatccggca
     1201 gcgtccccgc cacggcgacc gaacggagcg tgcgcctgat catgaccccc ctggacatcg
     1261 ggccgatgat gcgggacgcc gtctatgagc cgatcaagag cgcggtattc acgtcggcga
     1321 ccttgacggt cggcggcagc ttctcgttct ggtccaaccg catcgggctc gaccgcaacg
     1381 ttggacggga accgctcgcc cgcatcttcc cgtctccctt caactaccgc gagcaggtcc
     1441 tcctgggggt gccgacggac gcgcccacgc cggactctcc ccccttcgcc gcctacgtgg
     1501 cccgattcgt caccgaagcc gtcctggcga gccgaggcgc cgcgctggtg ctcttcactt
     1561 cctatagcct gctgcgggaa acccacgcgg cggcgttggc caccctcgcg caagcgggga
     1621 ttcgcatcct cagacaggga gaggacgatc gggcccgcct gctggaggcg tttcgatcca
     1681 agcgctccaa cgtcctgttc gccacggact ccttctggga aggggtcgac gctccaggag
     1741 acacgctgga gctcgtggtc ctgtgccggc ttccgtttcg ggtgccgagt gagccggtgc
     1801 tgaaggcgcg gatgatcgcg atcgaggccc ggggagggaa ccccttcagc gagctgtctc
     1861 tgccggacgc ggtcgtccgc ctgaggcaag ggttcgggag actcatgcgc cgccacgacg
     1921 acacgggtgt cgtgctggtg ttggattcgc ggatcgtctc caggacctac ggcgcggtct
     1981 tcatggatag cctgccggtg acccgcaggt ccatcgcccc gtcgtccgaa gtccttgccg
     2041 atgtacggga gttcttttcg cggagggaat cggagggaga agagggggag ggcggatagg
     2101 gccgccctcc ggctcgttgt ggagtgtgct actctacctt cgccaggata tcggatgcct
     2161 tgagaatcag ctgctcttcg ccgcccactt tgatggtcgt gccggcgtac ttgtcgtaga
     2221 tgacgcggtc cttcggcttc accttgatgg actcgtcttc gccaacggcc aggacgacgc
     2281 cttcctgggt cttctcctgc gcggtctggg gaatcaggat gcctccggcc gtcttggtct
     2341 ccatctccac cagcttcacg agaacccgat cgcccagcgg tgttaccttc atgtccagtt
     2401 gtcctccctt agactgtgta cggtgtacca agtatcactc caggcgcaaa ctcctgggtc
     2461 tagtgtgatt ttgagccgaa tataaccaga gcgccgcgga cgagtcaaga acgtagtgga
     2521 acgtctcgga tcgggccgct tgtcgccacg ccagcgctac ggtaccttat cggcggatgc
     2581 cggcgattcg ttgggacctg caagtgaagc gggggctatg ggccctttcc aaacaccatc
     2641 cgtcggaagc ggtacagctt ctgcagcagg ccctcgagaa ctgcccgccg tcccggacgc
     2701 aggagctcca ccggatctgc ttctacctcg gaatagcgct gagaaggctg ggcttctccc
     2761 agtccgcgat caagagctgg atctcctgcc aacggctcaa caagaggggg cacacccgca
     2821 agatgctcct tcgcctcacc aacgggtacg ggatggagcg gcaggatacg gttgaggggg
     2881 acgactggca ggccttctat tccattcagg cggcccggta ccttttgaac aaaaacaagc
     2941 ggaccttctc ggtggaagcg gagaaggaca tgatcggcga tctgatccga gacagctgga
     3001 accagctcca ggcatccgaa gcgctgactg acaagagcag ctgtgagaag ctggaagtct
     3061 tccgcagcgt cgtcattgtt ttcccgacca cggtagccgc ggagccaaag gccggggaat
     3121 ccgtcattgc ggtcaacttc cgtacgcgca ggaaagtagc cctcacggac cgctgcatct
     3181 gcggctccgg gttaccattc gtccaatgct gtgggcgcac acctggaaaa gaagagctcc
     3241 tgagtggtat attttgatgc agaaagcccg ccccttttcc attttgggtc agaatgacac
     3301 aagcttaaca tctccttcat aatccctcaa gaccctagct ctaattgttt attcgctaat
     3361 gacttgcaag acaccctttg gagccccatg gcatgcccct tgcttaacaa tggtcaagag
     3421 gtgaccatgg caactgcagt cgtagtggat cgcgagcgga agaacacgag gactgacgag
     3481 aacatcctct ccctctacct gaaggagatc aaccggatcc ccttgctcac gcgggaagag
     3541 gaggagcgac tggctcggag ggcggccaaa ggcgaacagc tcgccaagga gagcctgatc
     3601 cgcgccaacc tgcgcttc
//
LOCUS       DASRZS010000279        28484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1694137, whole genome shotgun sequence.
ACCESSION   DASRZS010000279 DASRZS010000000
VERSION     DASRZS010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 28484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28484
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694137"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VFH83_06845"
     CDS             complement(<1..972)
                     /locus_tag="VFH83_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486118.1"
                     /translation="MLRFRFLGIVAAVLFVVALPAMAATTVGIAGEVTYGAITNGTAA
                     SDAWVNAYMNITGTLDPNNSIELALQGTNMPTILGTGAETGQMVAPNITNLFLKSDLA
                     GDFGLDPKTVDPVLYAGYGVFDLPGYGLTQYGPEPIAAVGVDNGYNDGMFGSEIGAGY
                     GLIAMNTAIMGGLVNVALAASGTELRSSHQQALVGAWGAVGPVSYEVGWAQRGTASGY
                     VPVGLKFSYTVGPVALAAMGNYLQNLNTGGISNWSAGAQAVLMSNYTVDFSVMSYQPA
                     YEPTVSAMKGTGDVIVNITSNFGALASVYLNFDPNAASAFDTLEVSAW"
     gene            complement(1784..2368)
                     /locus_tag="VFH83_06850"
     CDS             complement(1784..2368)
                     /locus_tag="VFH83_06850"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486119.1"
                     /translation="MKQKIAIIGKGHVGSALERGLKGAGYETRMTGKGPDVQKTAAWG
                     DVVIWAVPFGVSAALASELGGTLSGKVLVDVTNVLGAQGQLALGYTTSGAEELQKKVP
                     AAKVVKAFNNVFAQNMASGTVQGQKIGCFVAADDAAAKSVVLQMARDIGFDAVDAGPL
                     ANARLLEPLGYLMIQIGYVINKGLGTAVGMSLTR"
     gene            2480..3541
                     /locus_tag="VFH83_06855"
     CDS             2480..3541
                     /locus_tag="VFH83_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6486120.1"
                     /translation="MADYRIGGILQIGVGVPETAGALRWCRRVLGTDVAVFDDVGEAS
                     LMLRYTGGAAQPRRAVLAVNLQGGGGLEVWQFTRRVPRPPADPVEVGDLGILAARIKS
                     RDPRRAYEALRAARTPSLGPLSEDPVGRPAFFIRDPLGLAYQVVEAEDWFAPGTHPTG
                     GIAGAMIGVAQVDRSLELYQGILGYDRVLYDVQGSFPDLSALPGGGGRFRRILLTHAA
                     PRTGPFSGLLGASCLELVQCLDRTPRRIFEDRFWGDLGFIHLCFDVGEMDSLKLRLET
                     AGFPFTVDSGGAFAMEDSSGRFAYIEDPDGTLLELVETYRLAIVKRLGWFLDLRRRPA
                     GQALPRWMLRALRMNRIRV"
     gene            complement(3546..4397)
                     /locus_tag="VFH83_06860"
     CDS             complement(3546..4397)
                     /locus_tag="VFH83_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6486121.1"
                     /translation="MPDSEIITTEQRGFFDRQGYLVVQGLFSGDEARSLSDHYMELRA
                     RGKYPGDSSGVSIGSNDPLKRYPRMIHMHRWDPISMDWLTDRRIAQTLTALLDREPYA
                     VQTMLYFKPPGARGQALHQDQYYLRVQPGTCVAVWLALDPCTEENGCLRVVPGSQGWP
                     LLCTQPADTTQSFTDVTVPVPAGTPVHSVIMEPGDTLFFNGQVVHGSGPNRSGSRFRR
                     SLIGHYIAGEARKVAQFYHPALRMDGSPVTLDTSEGGGTCGVWVDQKGKQVIEMVGSG
                     APTKASE"
     gene            4571..5620
                     /locus_tag="VFH83_06865"
     CDS             4571..5620
                     /locus_tag="VFH83_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6486122.1"
                     /translation="MAKARMGIGVLSWAHGHVGAYADRIRGFDDARLVSCWDDDPQRG
                     KANAAKFAMTFRSDRDEVLSDPDVECVMIGAETNKHAELCEAAASAGKAILLQKPIAT
                     TLEDADRIIRAVERSGVWFSLAWQMRCDPQNIHMRRLVRDGAIGRVGIVRRRHCIGLL
                     FNPSFLEGPSRWHTDRAANRGMWADDASHACDWLVWMFGRPRSVIAEIDNVLTHVAPD
                     DAGFAVFRYADGMMAEVHNASVTLASENTTEIYGDKGVIVQNHGDLVSCAVKPPHPLG
                     VKLYQAEKAALGWQDQGLAVPVSHGERISGVARPFVDALRSGQTLCSAQEARISLEMI
                     TASYTAAETGRRVMV"
     gene            5691..6350
                     /locus_tag="VFH83_06870"
     CDS             5691..6350
                     /locus_tag="VFH83_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486123.1"
                     /translation="MRKGLVLAVVLMALGAAAAFAFSSDELNKITFVNSTGSDIETLF
                     LSPGDSDHWGPDIIGADSVLANGDSLGFYVHYPETSFKFDIMASDTDGNTFEVYDYKL
                     TDGKEAKITFTKKNLNTSKPDFTFATLTITNKSSHELDYLFISPEDSNAWGVDLLNED
                     TTLSQGDSEKVMVPIGNAKVTYNLMAADDSDREYTFDLVLDPAKGKSFKASVEDSDLN
                     R"
     gene            complement(6358..7365)
                     /locus_tag="VFH83_06875"
     CDS             complement(6358..7365)
                     /locus_tag="VFH83_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6486124.1"
                     /translation="MTPRQRVQASLDHRQPDATPYHVTFTEPVRDAMRAYYGNQDFEA
                     GLGNCLEIIRTGLPMIEVTGRPGIWQDEFGVQWDRTIDADIGTVCNQPVRPETLREFR
                     FPDPHAPQRFAHFAPALEANAGRRFAIGTIGFTLFERAWTLAGMENVLASMVDDKGFV
                     NELLDRILDFDLEVTRKALSYDIDGMRFGDDWGQQRGLIMGPVLWRELIKPRIARLYA
                     LVHAAGKRVFIHSCGKVDELFPDLIEVGVDVFNPFQPEVMDVEEMKRLHGERISFFGG
                     ISTQRTLPYGTVEQVRTEVRTLIRTVGNGGGYIAAPAHDIPKDAKPQNVAAMLEVLQT
                     Q"
     gene            7598..12289
                     /locus_tag="VFH83_06880"
     CDS             7598..12289
                     /locus_tag="VFH83_06880"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET6486125.1"
                     /translation="MGKRIAAAALMLASALVAFAGGSPESSTYTPVNGLENWDTSVDL
                     NGKKEGKYNLIVRATDEAGNVRYAGPFNIVVDPNSDLPLAHISYPTPGARVSSSLNVV
                     GTCIDDDGVQQVMVSLDGAEAQQAKGTGFWSMSFDVQQIDDGVHILSARGVDINGKVG
                     PAQNVTFNVDKNPPSTRILSHASGSLVSGQVTFTGEVQDANGVESLQMSTDGAKSYRA
                     VPLSLDKTRRTGTFRLGIDTRKTPDGPQIIWFRATDRMGSVGLYTFLYFVNNEAPSLE
                     ILSPSDKAVVHGKLIIAGKASDKIGLKSLSWDMGGDGKGTIDLVPGNPFWTQPLDLSA
                     RKAGTVQITYTLENLTGIRQTRKVTLRVDPEAGRPRITVASPSRGARFFGPVAVYGFA
                     SDDTAVDHVEYSLDGAAWTKVPARTGFSFQLSDVAAGSHKLSLKAVDVDGMPSTVAEA
                     PFTETSAPAVITVSGFQAAAGILPFAPGMLWAGDKDAKVTGSIVVSGPSVKAEYSLPG
                     ASGRSLSLKQGSKPAEKLFEVSLPKSLTAGKIDLSIKATDGLGVTSEYRTFLFKGSVS
                     GGPGIVLVDSRITPEGAIALNDQPLVGYLIGGAVQSATFDPPTQLLQVQTDGQLFRVT
                     AAASGVTEPTRVQITSSDGSAYSTDPLRFITDRDPPQLSVDRPSAGDWTVGSVDLAGS
                     ARDVGGLAQVAYAVDSGDPQPLTVAAGDSGGSFHDTVSLASVEDGDHLILVSATDKAG
                     NVTRLAIPFRKDTTPPTLTLVAPRAQDPVNGTLSLVGAVEDEGQVALVELSQDGKTFQ
                     QVGRERQFKVDVNLSALGDKANELQFRCTDAAGNVAVVRPQLTLDLAADVPVCQIQLP
                     APGQVMRSDFELSGMAFDDDGVASILYRIDGGPFVTLPGGNSYRAQISLADVTDGPHT
                     VDVKAVDIGGLESPLVSSQFIVSKSDPVSTLDSPDITAHLRGEIELKGSSHDPNGIAT
                     VEISLDNGQSFLQVDGKESWRYRLNTGMLSDGTHEVMVRAVDTTGAVGLFATTINVDN
                     TPPTLVVDSPKDGDVYTQNLTLNGRTFDNLGPASLAVTMAPLDGPAKDGPKVLEAKLT
                     AGGILRQEVDLKDVPPGRYNIQLQASDQAGNRTVVSRNVTRQASQEAESVVLYFPTGG
                     ERVSGPFTVSGRVFSRATTAEVTARVLVDGQLLDQVPLQERGTFHLEVVEGALSESSH
                     TITVEAQLPGGDRLLSDTRLIRYETSGPWVKITAPTPGAYITSRPYLKGEAGWLEATT
                     TPDAGATPGASATAPSPSSGSAGKPKVDPGHALKVVEVSMDNGSSFARAEGTASWRFR
                     LETQDMTNGELRLLVRSTYANGDTAVTRTILTVDTRPPQVTLLQPSEGERFNENVELV
                     GTASDESGLKDVGVSFRTGDKANYEVPSFIQGLYLDTHLLGATYWDAGMGLTFFSDAV
                     KLQAQVGMSPPGRFSGLVIGGKLLANVATLPFSYLFGPDWSFFSMSLAVGADFSYFTM
                     SGDTVSLTDTGAMLAGIVAQLEFAKFRIPGWTFANTYSLYTELQVWFISSDVQATTVP
                     RLSFGVRVGLL"
     gene            12400..13293
                     /locus_tag="VFH83_06885"
     CDS             12400..13293
                     /locus_tag="VFH83_06885"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6486126.1"
                     /translation="MDSSSRYRVLSPEQERQFLEKGYLVVEGCLDPELARRWTDRAYQ
                     RLGCSRDDPESWPPGITWMDHETRMPVRTVSPRAWAAICDVAGGEERLETKVLGLPNS
                     GHFTTMNSFEWSDAFIVNFRNGSELPWIPPSPALSGWHKDGSYFRHFLDSREQSLLTV
                     VLWSDVQPQGGGTFIAPDSVRIVARYLYEHPEGVEPNGFGRLIDQCKEFVEISGPTGT
                     FAILHPFMLHASSRNHSGRPRFMSNPPIILKDHQNLNRSDPREFSLLERATLHALGME
                     RCDFRPTAPRRSDWWVVGEGS"
     gene            13290..14084
                     /locus_tag="VFH83_06890"
     CDS             13290..14084
                     /locus_tag="VFH83_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486127.1"
                     /translation="MKILWGVVACALALLCGCVDTTTNITLKPDGSGTIDKTIVLSKH
                     LAEFVLKSGMSGDAASVERSMLNENQLKGAAGQMGTGVSCVSVDKITAEKGNGYHVVY
                     AFKDISKVKINTNPASDLNMPAGGAAAAPAQYLTFKFASGSPATLTVVAPKPADAKPT
                     TPARQMSQADADKMYATMKPLYADLRILFTITVAGKIASTNAAYADGSTVTLTDMDFA
                     KILADDATVKKLRAAQSQSMDQVRDIVKGVPGVKLETQDTVTVRFE"
     gene            complement(14100..14366)
                     /locus_tag="VFH83_06895"
     CDS             complement(14100..14366)
                     /locus_tag="VFH83_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486128.1"
                     /translation="MSFLLALFAVIEHINKSELTTSGKKLVMAYLKDAGGATYSEKAR
                     ATVHRYAMTVLPSLDSIRTKSKTMRLDALDRLVLKLEREATTVR"
     gene            complement(15066..15635)
                     /locus_tag="VFH83_06900"
     CDS             complement(15066..15635)
                     /locus_tag="VFH83_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6486129.1"
                     /translation="MLVPGIWDSAEAYHRMAARLSAAGWSVFAVSLHPSDGSVRMEQS
                     AAELERLIEEKLGVQRPFDLVGFSMGGIIARYYAQRMNGLDRILRLVLISTPNHGTRT
                     AYLESLSGVTELRPGSTLLADLAVDEGRLASIPVTSIWSPYDLAIEPPTSSRMPFGRE
                     VVLPVLSHAWMLTDDRTIQAVIEALEQPR"
     gene            complement(15745..16938)
                     /locus_tag="VFH83_06905"
     CDS             complement(15745..16938)
                     /locus_tag="VFH83_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6486130.1"
                     /translation="MTIAERIGRLGSETAFAVSAEAAEFAAKGNTVYPFHLGDLNLPT
                     PQDVMDASVKAMRDGKTGYCPTAGIPQLREAVAADVAASHHVPYTLENVAIQPGGKPT
                     IGKFFLALMNPGDEVLYPNPGYPIYESQIEFLGGKALPYWYVEGKDGFRLDFQSIEKQ
                     ITPKTRLLVLNDPHNPMGSECSPVELERIAELVLKHGLYVLCDEAYYDMRYSGTGISL
                     ASLPGMAERCVILYTFSKKFAMTGWRLGATIGPREVIEVIAKLNVNDESCSNHFIQYG
                     ALAGLRGNQEGPRRILSILRERRDVAVDILNTIPGIRCFRPEVTFYLFPNVTGAMRRI
                     GLLDYTRFRQTVLRETGVSFCTRAHFGRVLPGEREFYIRLAYSGIDAPLIRDGLARLK
                     KFLDR"
     gene            17261..17548
                     /locus_tag="VFH83_06910"
     CDS             17261..17548
                     /locus_tag="VFH83_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET6486131.1"
                     /translation="MNKKLYVGGLAYSVNDDQLRQLFASHGSVQSAKVITDRDTDRSR
                     GFGFVEMGTQEEAEKAIAALNGTQFEGRTLTVNISRPREERPARSGGRDRY"
     gene            complement(17647..18255)
                     /gene="pdxT"
                     /locus_tag="VFH83_06915"
     CDS             complement(17647..18255)
                     /gene="pdxT"
                     /locus_tag="VFH83_06915"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET6486132.1"
                     /translation="MRIGVLALHGDFAEHLAMLGSLATQRTADWGSVVAVEVKLPQQL
                     DSLDGLILPGGESTTIGKLMVDYGLVEPLRARVRSGLAVFGTCAGAILLARDLGGSTQ
                     PRLGVMDLSIRRNAYGRQIQSFETDVAVSGIGGPPLRAVFIRAPAIVGCGPSVRVLAS
                     LPNGSIVAARQGRLLAAAFHPELTGDSRLHQYFLSMVEGQAA"
     gene            complement(18258..19139)
                     /gene="pdxS"
                     /locus_tag="VFH83_06920"
     CDS             complement(18258..19139)
                     /gene="pdxS"
                     /locus_tag="VFH83_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET6486133.1"
                     /translation="MEQSTWITKKGLAQMLKGGVIMDVVTPEHARIAEEAGACAVMAL
                     ERVPADIRAAGGVARMSDPEMILQIMEAVTIPVMAKCRIGHTVEAQILQAIGIDYIDE
                     SEVLTPADDAHHVDKHAFQVPFVCGCRDLGEALRRIAEGAAMIRTKGEAGTGNVVEAV
                     RHMRAVQGALRHLRSRGAEELMAEAKALNASYDLVVLVAQEGRLPVVNFAAGGVATPA
                     DAALMMHLGADGVFVGSGIFKSENPARRAAAIVRAVTHFQDPAILAEVSKGLGEAMAG
                     VEISTLAPDKLLAVRGG"
     gene            19249..20799
                     /locus_tag="VFH83_06925"
     CDS             19249..20799
                     /locus_tag="VFH83_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET6486134.1"
                     /translation="MRADDLMIRLTPAPGRGTPTTRPRSTLGSAAVPRRPYHRQIFDG
                     IREAILAGRLRPADRLPATRELANELGVARNTAARAYEDLLAAGYLEARVGAGTYVSS
                     GIRPPVGRGSTPPVGRGSTPLLGPPAPRSAQVPARAADPLRFDFLPGIPDWDAFPRAL
                     WHRLLGRALRARPSELRRYGDPAGYFPLREAIARYLAVSRGTVATPEQVVIVSGSQQA
                     LDLIARRVLSGRDTAAIEDPGYPEARRLLSAAAARLCTVPVDDQGMVVERLATAAGSD
                     PRLVYLTPSHQFPTGATLSLARRLALLDWAGRRDALIVEDDYDSEFRAPGRTIESLQG
                     LDRGHRVIYIGTFSTVLFPPLRVGYVVLPPSMVEPFVLTKWQADRQTPTLEQIALTDF
                     ILEGHFERHLRRMRRLVAARRRAMLAAITDRLGDVEVAGADSGMHLMMRLPPLTGHAA
                     REVEERVSAAAAELSIAVYPVAPCRSGPVRQAAFALGYAAVSEEIIREGISAFAGAIR
                     RVYRDGHR"
     gene            complement(20883..21854)
                     /locus_tag="VFH83_06930"
     CDS             complement(20883..21854)
                     /locus_tag="VFH83_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET6486135.1"
                     /translation="MNSKVRIVVLDGYALNPGDNPWDPVSALGEITVHDRTPEAEVVR
                     RAGSAEVVLTNKTPLSQRSIEALDRLRFVSVLATGYDVVDVKAARARGIPVANVPEYG
                     TESVAQFVFALLLELASGVGEHNRAARDGRWTASGDFSVTVQPLVELAGKTMGIVGFG
                     RIGRRVGEIAHGFGMKVVALDPFRERPPEYQPFSWADRAEDLFQQSDVLSLNSSLNAS
                     NARFVDRSLLSHAKPTAFLINAARGGLVNEADLAEALNQGHLAGAALDVVSQEPIRND
                     NPLLTAKHCLLTPHIAWASLEARRRLMAATAANVRAFLSGNPQNVVN"
     gene            22026..22661
                     /locus_tag="VFH83_06935"
     CDS             22026..22661
                     /locus_tag="VFH83_06935"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HET6486136.1"
                     /translation="MTANTLRIQKLSLVGGRLCLDFTNTVSWRGAENPRDLLQSYEDL
                     VTWGVRADLLDSTSAGALLARASSEAQAAELALAKAKALRESIAGIYDAVASGGNPKD
                     DDLSRLTTFVRDAFSRVQLCRSESGYDIRFERAGALDQLLPPIAWSAAELLRDPALTR
                     VKCCSGRTCGWLFVDSSKNHSRRWCEMKDCGNREKARRHYHKTRIQGTGAT"
     gene            22714..23997
                     /locus_tag="VFH83_06940"
     CDS             22714..23997
                     /locus_tag="VFH83_06940"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HET6486137.1"
                     /translation="MSFLITGYSVALWGSAAVSTVTAAVAYRRRAAPGGRALALMLVC
                     VAVWGSMAGFESAAVGLPLKIRFSQLTYVGVVGVAPLFLLFSVAYTGRKPFRLGISLL
                     LWVVPLVTVALAFTNGSHGLVWKSFTADPSPQRNLVYYGHGPWYWVSLGYYVALVLGA
                     TVIILHASIRAPRIYLRQTLVMIAGVVVPWLGEVLYVSPRNPFPGLDLTQIGFAVTGI
                     LLLYGFSRLRLFDVVPMARSLLVERMRGGLVVLDLKGRLVDLNAAARQMLRAEDAAVG
                     KPAALALPALAAHLPAPGTAPEDQTREIPMPGTEDRRLELAVSSLRDPMGRSGGVLIV
                     LQDVTARHRMEQEREKLIGELTKALADVRTLQGLLPICASCGKIRNDRGYWENIEHYL
                     AAHTGAQFTHGLCEECIHMLYPELDEPPPSADAPP"
     gene            complement(23994..24788)
                     /locus_tag="VFH83_06945"
     CDS             complement(23994..24788)
                     /locus_tag="VFH83_06945"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6486138.1"
                     /translation="MTALKKLTAEETARYKTEGYLVYRHPVLTDARFRSLKDHFEKKL
                     AALDPGKRGEHMDVPHFLDTALFEWLFDDEVLDLVEDILGPDIDLFSSHFISKSAGDG
                     LRVPWHEDSAYWRDMLTPMEVVTVWLALDSSDEGNGCMYVIPRTHDNGFSQYYNVDPN
                     KNLFMTEIRKGQFDEKKAVPIVLAENEASLHHAKLIHGSPANTSTRRRTGYTMRYIAS
                     STRLTPENLARHQLYHARGKDYGINRYGDPQKTYPDLMKARTLKGH"
     gene            complement(24899..25654)
                     /locus_tag="VFH83_06950"
     CDS             complement(24899..25654)
                     /locus_tag="VFH83_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018752455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HET6486139.1"
                     /translation="MAFLQCDFFSATLALSTSMNVVLPLGEGVKRPYRTLYLLHGLSD
                     DHTIWARRTSIERYADAHGVAVVMPAAGRSFYVDMANGARYGAFFEEELPSVTRSLFP
                     LSPRREDTFVAGLSMGGYGAFRLALRRPDLFCAAGSFSGALNMAKVVEESDEERKAEW
                     QRIFGDAGGVAGSDRDLFHLADALPPAARARLRLYQCCGTEDFLYQDNLLFRDHARRL
                     GLDLSFSDGPGDHEWGYWDRQVKDFLDWLLKDS"
     gene            25653..26870
                     /locus_tag="VFH83_06955"
     CDS             25653..26870
                     /locus_tag="VFH83_06955"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HET6486140.1"
                     /translation="MAKPTGNAARRAMPVVEWPSLEGCMRITRTIVACVCGLLILVVA
                     ALVGSIAFMRSTRRADFTHQRDRIEALASARPDALAPAGPETPGPGAGPAKAGDTPAV
                     DRAVRDDLRLNQIRMVATHNSYHVQSDPLRLFLIGLFEPVWPARLRYSHAPLSEQFDH
                     GVRSVELDVRNQGSRFVIAHVPLVDDRATCPDFLLALREIRLWSERHPDHLPIVVLLE
                     LLNDWSFLDPRLRDFDRPALDRLDAEIHDAFPAPELLTPDNVRAGAVTLEAAVVGLGW
                     PRIGEIRGRVMFVMHDNETYRRLFVEGNPTLAGRSLFTCAPSGNPDAAVRVLDDPVAD
                     GPTIASLTHTGYLVRTRADADLDIEPAVAQAAVASGAQIVSTDYPPSEPRTDTGYRFS
                     FPGGRMVTARMGP"
     gene            27032..28270
                     /locus_tag="VFH83_06960"
     CDS             27032..28270
                     /locus_tag="VFH83_06960"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6486141.1"
                     /translation="MSNSFSAPAPACRVLVVDDEKWIRHALWVYLSRDGSVVETARSA
                     VAALTLASRRNYDVLLVDLEMPGTDGLSLLGELTRSCGQALCIALAASPEEPRPAEAL
                     KLGAASCLLKPLDGEALAEIARWIAVRNGLTRAPLGHRVTDPEREVVAESPTSQAAFL
                     QVSRAAPLSGPILLEGESGCGRGFLARLAHLWGPGSSGRLVRLDCPRASESLLESALE
                     SARGGGLYLEEIGKLPRVLQGRLAAALADRDGRGGRHTRLIASTSQDLRRETRLGRFH
                     GELYRRLDETRIRVPRLRDRPQDILPLACRFLRQSDLRSGKPAHGISADAVRFLQTQR
                     WRGNLHELIDAVACAAACAEGVDVTARDLRAYRARSNLLWRPYLANARNGLSRVIRRM
                     VGSGTPEEAKAGRKACTRYA"
BASE COUNT         4807 a         9489 c         9319 g         4869 t
ORIGIN      
        1 ccaggccgaa acttccagcg tatcgaacgc cgacgccgcg ttgggatcga agttcaggta
       61 gaccgacgcg agggcgccga agttgctcgt gatgttgacg atcacgtcac ccgtgccctt
      121 cattgccgac acggtgggct cataggccgg ctgataggac atcaccgaga aatccacggt
      181 gtagttgctc atgagcacgg cctgcgcacc cgcggaccag ttggatatcc ctcccgtgtt
      241 caggttctgc aggtagttgc ccatcgccgc cagcgccacg gggccgaccg tgtagctgaa
      301 cttcagtccg acggggacgt acccggaggc tgttcccctc tgcgcccaac ctacctcata
      361 gctgaccggg cccacggcgc cccaggcgcc gaccagggcc tgctgatggc tcgagcgcag
      421 ttcggttccc gatgccgcca gcgccacgtt gaccaggcct cccatgatgg cggtgttcat
      481 cgcgatcaat ccgtatcccg ccccgatctc gctgccgaac atgccatcgt tataaccatt
      541 gtctactccc acggctgcga tgggctcggg tccgtactgc gtcaagccgt aacccggcag
      601 atcgaacacc ccatagccgg catagagaac agggtccacc gtcttgggat ccagcccgaa
      661 atcgccggca aggtccgact tcaggaagag gttggtgata ttcggggcga ccatctgtcc
      721 cgtctcggcg cccgtgccca ggatcgtcgg catgttggtc ccctgcaacg ccagctcgat
      781 cgagttgttg ggatcgaggg tccccgtgat attcatgtag gcgttgaccc acgcgtcgga
      841 cgccgccgtg ccgttcgtga tggcgccgta ggtgacttcc cctgcaatcc cgaccgtcgt
      901 ggctgccatg gcaggaagcg cgacgacgaa cagcaccgct gccacaatcc ctagaaacct
      961 gaacctcagc atgcgtagcc tcccgaaaca attgctaccg gagccaacgg ctccccattc
     1021 ataacccgcg ggacacgagg ctctccgtgt cgcggatcga gcgagggtca cgagggagcg
     1081 cgctctggtt gttgagaccg ggcaccactg catgtcgaag tggggcttcc tctgaccacg
     1141 aaagacttgc ggtggttgcc gggcggcacg gtcgctgccc gaaacgcaaa ccgcaagagc
     1201 gccgcacggt tgtttcccct gcatccccct ggtcagaccg atctacgtga ggccaacggc
     1261 cccttatcct attcatgccg gcatggaccg gcccgcctgc gagataacct ccctcgtgga
     1321 atcgtgagga ggcggcgcgg cactgagccg gccgcgacct ccctcggcgc ccttctcgac
     1381 ccaccgggcc aacggcccgt tcgacacggg tcgatgggaa gacgccgcgc agcttcccgc
     1441 tgcgagccgg atgagggccg cgatacgcgg tgcgccgcgg ggtgaagccg cgcgctctat
     1501 cgtccgcgcc cacggaaagg gaggaagtga atgcgataac gatgccccga acgcggccct
     1561 cacccggccc acgctggtac gacaactgcc cttccgcacg ttcgatcttg cgccggagca
     1621 atcccgaata ccataaatgg cggactagat ttcgatgaac gccggattct ataaaggtgg
     1681 gttataaaaa ctgctggtta taaaacggcc cgacgggcgt cgaaggcgca cttcacccga
     1741 gcgttgccga gagtgagatc gcgtgacggt gaggccgcgc cgactatcgc gtgaggctca
     1801 tcccaacggc ggtgcccagc cccttgttga tcacgtagcc gatctggatc atgaggtagc
     1861 cgagaggctc caaaagacgg gcgttggcca gaggcccggc atccaccgcg tcaaaaccga
     1921 tgtccctggc catctggaga acgacgctct tggcagcggc gtcatccgcg gccacgaagc
     1981 agccgatctt ctggccctgc accgtgccgc tcgccatgtt ctgagcgaag acgttgttga
     2041 atgccttgac caccttggca gcaggcacct tcttctgcag ctcttcggcg ccgctcgtgg
     2101 tgtagcccag ggcaagctgc ccctgcgctc ccaggacgtt ggtcacgtca accaacacct
     2161 tcccagagag ggtgcctccc aactccgagg ccagagcggc gctcacgcca aacgggacgg
     2221 cccagatcac cacgtcgccc cacgcggcgg tcttctgcac gtcgggcccc ttgcccgtca
     2281 tgcgcgtctc gtacccagct cccttgagcc cgcgctccag cgcgcttccc acgtggccct
     2341 tgccgatgat ggcgatcttc tgcttcatga tggatctcct cccgctcggg aagatacgca
     2401 aaagtctgca cggcgggcga tggggcgatg agggggaggc gcgacgacgg tgtcgaattg
     2461 cagtagattg atccggccca tggcagacta tcgcatcgga ggcatcctgc agatcggggt
     2521 tggagtcccg gagacggccg gagcgctgcg ctggtgccgc cgcgtcctgg gaacggacgt
     2581 cgccgtgttc gacgacgtcg gggaggcatc gctgatgcta cggtacaccg gaggcgccgc
     2641 gcagccccgg cgcgccgttc tggcggtcaa ccttcagggc ggcggtggac tggaagtctg
     2701 gcagtttacg cgccgtgtcc cccggcctcc cgctgacccc gtggaggtcg gtgacctggg
     2761 catcctcgcc gcgcggatca agtcccgcga tccccgccgg gcgtacgaag cgctgcgcgc
     2821 cgccaggacc ccgtccttgg ggccgctgtc ggaggatccc gtcggtcggc cggccttctt
     2881 catccgagac ccccttgggc tggcctacca ggtcgtggaa gcggaggact ggttcgcacc
     2941 ggggacccac cccacggggg ggattgctgg cgctatgatc ggggtagcgc aggtcgatcg
     3001 ctcgctggag ctctaccagg ggatcctcgg gtacgaccgg gtgctctacg acgttcaggg
     3061 gagcttccca gatctctccg cgcttccggg cgggggagga cggtttcgaa ggatcctcct
     3121 cacgcacgcc gccccccgga cgggaccgtt cagcggcctc ctcggcgcga gctgcctgga
     3181 gttggtgcag tgtctcgatc gaacgcctcg ccgaatcttc gaggatcggt tctggggaga
     3241 cctgggattt atccatctct gcttcgacgt gggagagatg gactccctga agctgcggct
     3301 ggagaccgcc ggattcccgt tcacggtgga cagcgggggc gcctttgcca tggaggactc
     3361 cagcggcagg ttcgcctata tcgaggatcc cgatggcacc cttctcgagc tcgtggagac
     3421 ctaccggctg gccatcgtga agcggctggg ttggttcctg gatctgcgcc ggcggccggc
     3481 gggccaggcc cttccccgct ggatgctgcg cgcgcttcgg atgaaccgga tccgcgtctg
     3541 agtctctact cgctcgcctt ggtcggcgct cccgagccca ccatctcgat gacctgcttt
     3601 cccttctgat ccacccagac gccgcaggtg ccgccccctt cactggtatc cagggtcacc
     3661 ggcgatccat ccattcgcag cgccgggtgg tagaactggg ccaccttgcg ggcttccccg
     3721 gcgatgtagt ggccgatcag ggacctgcgg aacctgctcc cgctgcggtt cggtccgctc
     3781 ccgtgaacca cctgcccgtt gaagaacagc gtgtcccccg gctccatgat caccgagtgg
     3841 accggtgtgc ccgcgggcac gggcacggtg acgtccgtga agctctgcgt agtgtccgcc
     3901 ggctgtgtgc agaggagcgg ccagccctgg ctccccggca ccacgcgcag acagccgttc
     3961 tcttccgtgc acgggtccag ggccagccac accgccacgc aggtccccgg ctgaacccgc
     4021 aggtagtact ggtcctggtg gagcgcctgg ccgcgcgcgc cgggtggctt gaagtacagc
     4081 atggtctgca ccgcgtaggg ctcgcggtcc aggagcgcgg tcagcgtctg ggcgatcctg
     4141 cggtcggtaa gccaatccat gctgatcggg tcccatcgat gcatgtggat catccggggg
     4201 taccgtttca acgggtcgtt gctcccgatg ctcaccccgg agctgtcgcc aggatacttc
     4261 ccccgggcgc gaagctccat gtagtgatcc gacaggctcc tcgcctcatc cccggagaaa
     4321 agcccctgga ccaccaggta tccctgccga tcgaagaacc ccctctgttc agtcgtgatg
     4381 atctcggaat caggcatgtg gcactccttc ccaaacggct cccccgctcc ccggctttgg
     4441 agaagatagc cagcccggag gccaggggca aaaccggggc ttccagggga gtccgccgct
     4501 ggctacactt gccgcgatcg ctcgggccgt cgtcccgtgc cggtgtttct ctcgaacaag
     4561 gaggctttcc atggcaaagg cgcggatggg aatcggtgtg ctgagctggg cacatgggca
     4621 tgtcggcgcc tacgcggatc ggatccgagg tttcgacgac gctcggctgg tgtcctgctg
     4681 ggacgatgat ccgcagcgtg gcaaggccaa cgcggccaag ttcgccatga ccttccgttc
     4741 ggaccgcgac gaggtcctct ccgaccccga cgtcgagtgc gtgatgatcg gggcggaaac
     4801 caacaagcac gcggagctct gcgaagcggc ggcatcggcc gggaaagcca tcctgctgca
     4861 gaagcccatc gccacgacgc tggaggacgc ggatcggatc atccgggccg tggagcgctc
     4921 cggggtgtgg ttctctctgg cgtggcagat gcgctgtgat ccccagaaca tccacatgcg
     4981 caggctcgtc cgcgacgggg cgatcggtcg ggtggggatc gtccgccggc gtcactgcat
     5041 cggcctgctg ttcaacccat cgttcctcga aggaccgagc cgctggcata cggatcgggc
     5101 ggccaaccgc gggatgtggg cggatgacgc ctcccatgcc tgcgattggc tggtctggat
     5161 gttcggaagg ccgcggtccg tgatcgcgga gatcgacaac gtgctcacgc acgtggcgcc
     5221 ggatgacgcc gggtttgccg tcttccgcta cgccgacggg atgatggcgg aggttcacaa
     5281 cgccagcgtc acgctggcct cggagaacac cacggagatc tacggggaca agggggtcat
     5341 cgtgcagaac cacggagacc tggtctcctg cgcggtgaag ccgccccatc cgctcggtgt
     5401 caagctctac caggccgaga aagccgcgct gggctggcag gaccagggtc ttgcggtgcc
     5461 cgtgagccac ggggaacgga tcagcggcgt tgcgcgtccg ttcgtggatg ccctgagatc
     5521 gggccagacc ctgtgctcgg cccaggaggc gcggatctcc ctggagatga tcacggcgtc
     5581 gtacacggcc gcggagaccg gcaggagggt gatggtgtag agcggcgtcc ctgaaccggg
     5641 ccacccctcg cgctatactt ctggttcgtg accgaaaagg gaggttctgc atgcgcaagg
     5701 ggctggtact cgcggttgtt ctgatggcgc ttggcgccgc ggcggcgttc gcgttcagct
     5761 cggatgagct caacaagatc acctttgtga actcgacggg gagtgacatc gagaccctgt
     5821 tcctgtcccc gggggacagc gatcactggg gcccggatat catcggggcc gactcggtcc
     5881 ttgcgaacgg tgactccctc gggttctacg tccactatcc cgagacgtcc ttcaagttcg
     5941 acatcatggc ctcggacacc gacggcaata ccttcgaggt gtacgactac aagctcaccg
     6001 atggcaagga agcgaagatc accttcacca agaagaatct caatacgtcc aagccggact
     6061 tcaccttcgc cacgctcacc atcaccaaca agtcctctca cgagctggac tacctgttca
     6121 tctctcccga ggattccaac gcctggggcg tggacttgct caacgaggac accaccctga
     6181 gccagggaga ctccgagaag gtcatggtgc ccatcgggaa cgcgaaggtg acctacaacc
     6241 tcatggccgc ggacgacagc gaccgcgagt acaccttcga tctcgtcctg gatccggcca
     6301 agggcaagag cttcaaggcg agcgtggagg acagcgacct caaccggtag gacgcgccta
     6361 ctgggtctgg agcacctcca gcatggcggc cacgttctgt ggcttcgcgt ccttgggaat
     6421 gtcgtgagcc ggggcggcga tgtagccgcc cccgttgcct acggtgcgga tcaacgtccg
     6481 cacttccgtc ctgacctgct cgacggttcc atacgggagc gtgcgctgcg tgctgatccc
     6541 gccgaagaag ctgatccgct cgccgtggag gcgcttcatc tcctccacgt ccatgacctc
     6601 gggctggaac gggttgaaga cgtcgacccc gacctcgatc agatccggga acagctcgtc
     6661 caccttgccg cagctatgga tgaacacgcg cttgccggcg gcgtgcacca gcgcgtacag
     6721 cctggcgatc cgcggcttga tgagctcgcg ccagaggacg ggccccatga tcagcccccg
     6781 ctgctgtccc cagtcgtcac cgaagcgcat cccgtcgatg tcataggaca gggccttgcg
     6841 ggttacctcg aggtcgaaat ccaggatccg gtcgaggagc tcgttcacga accccttgtc
     6901 gtcgaccatg gacgccagga cgttctccat accggccagg gtccacgcgc gctcgaagag
     6961 cgtgaagccg atggttccga tggcgaaccg ccggcccgcg ttggcctcga gggcaggagc
     7021 gaagtgagcg aaccgctgcg gggcgtgcgg atcggggaag cgaaactccc gcagagtctc
     7081 gggacgcacc ggctgattgc acacggtccc gatgtccgcg tcgatcgtcc tgtcccactg
     7141 gactccgaac tcgtcctgcc atatgccggg cctgcccgtc acctcgatca tcggtagtcc
     7201 cgtccggatg atttccaggc agttgcccag cccggcttcg aagtcctggt ttccgtagta
     7261 cgcgcgcatg gcgtcccgca ccggttcggt gaaggtgacg tggtagggcg ttgcgtccgg
     7321 ttggcgatga tcgagagaag cctggacgcg ctggcggggt gtcatgggca ttcctccggg
     7381 ttccttcact gtgcgacccg gtcccggacc tgtcaatgac cggattcccc ggtgagagaa
     7441 tccgaccacc gggggctaca ttgagaccga ttcggaaaca ttccactcga ggtgcggggg
     7501 caggcgggca tgcgtggtgg gacccgcggc cccgccgttc gtttgtgccc tcgggatggg
     7561 ggagctcagg ctctggatcc ccgtgaggag tcgttctatg ggtaaacgga tcgccgccgc
     7621 ggccctcatg ctggcttcgg cgctcgtcgc gttcgcgggc ggaagcccgg agtccagcac
     7681 gtacacgcca gtgaacggct tggagaactg ggacacctcg gtcgacctca acggcaagaa
     7741 ggaagggaaa tacaacctga tcgtccgcgc cacggacgag gccgggaacg tccgctacgc
     7801 gggaccgttc aacatcgtgg tcgatcccaa ctcggatctc ccccttgccc acatctcgta
     7861 tcccaccccg ggcgcccgcg tcagctcctc cctcaacgtc gtcggcacgt gcatcgatga
     7921 cgacggcgtc cagcaggtga tggtctccct ggatggcgcc gaggcccagc aagccaaggg
     7981 caccgggttc tggtcgatga gcttcgatgt ccagcagatc gacgacggcg tgcatatcct
     8041 ttccgctcgc ggcgtggata tcaacggcaa ggttgggccg gcgcagaacg tcacgttcaa
     8101 cgtggacaag aatccgccga gcacccggat cctgtcgcac gccagcggct ccctggtatc
     8161 cggccaggtc accttcacgg gggaagtcca ggatgcgaac ggagtggaat cgctgcagat
     8221 gagcacggac ggagccaaga gctaccgggc ggtgcccttg agcctggaca agacgcggcg
     8281 caccggcacc ttccgcctgg ggatcgacac gcgaaagacg ccggacggac cccagatcat
     8341 ctggttcagg gccaccgacc ggatgggctc ggtgggcctg tacaccttcc tgtacttcgt
     8401 gaacaacgag gctccttccc tggagatcct ttctccctcg gacaaggccg tcgtgcatgg
     8461 caagctcatc atcgccggga aggccagcga caagatcggc ctcaagtccc tgagctggga
     8521 catgggaggc gatgggaagg gcaccatcga cctggtgccg ggcaatccct tctggacgca
     8581 gccgctggac ctgagcgcgc gaaaagccgg gacggtccag atcacctaca ccctggagaa
     8641 cctcaccggc atccgccaga cccgcaaggt cacgctccgc gtggaccccg aggccgggag
     8701 accccggatc acggtcgcct ctccctctcg cggcgcacgc ttcttcgggc cggtggccgt
     8761 ctacggattc gcctccgatg acaccgcggt ggaccatgtc gagtactcgc tcgatggagc
     8821 cgcgtggacc aaggtccccg cccgcacggg gttcagcttc cagctttcgg acgtggcggc
     8881 ggggagccac aagctttccc tcaaggcggt ggatgtcgac gggatgccca gcaccgttgc
     8941 ggaggctccg ttcacggaga cctccgctcc cgccgtcatc acggtgagcg ggtttcaggc
     9001 ggccgcgggg atcctgccgt ttgcgcccgg catgctgtgg gccggcgaca aggatgcaaa
     9061 ggtcaccggc tccatcgtgg tcagcggacc gtccgtgaag gcggagtaca gtctccccgg
     9121 cgcatcgggc cgttccctgt cgctgaagca gggctccaag ccggccgaga agcttttcga
     9181 ggtaagcctt cccaagtcgc tcacggccgg aaagatcgac ctgtccatca aggcgaccga
     9241 cggcctcgga gtcacctcgg agtaccgaac cttcctgttc aagggctccg tctcgggggg
     9301 tcccggcatc gtcctggtgg attcccgaat caccccggaa ggcgcgatcg cgctgaacga
     9361 tcaacccctg gttggctacc tgatcggggg cgccgtccag agcgccacgt tcgatccacc
     9421 tacccagctc ctccaggtgc agaccgacgg ccagcttttc cgggtgaccg cggcggcctc
     9481 cggcgtgacc gagccgaccc gagtgcagat cacgtcatcc gatgggagcg cctatagcac
     9541 ggatcccctc cggttcatca cggaccgcga ccctccgcag ctcagcgtgg atcggccctc
     9601 cgcaggagac tggaccgtcg gttccgtcga tctcgcgggc tcggcccggg acgtgggtgg
     9661 tctggcccag gttgcgtacg cggtggacag cggagaccct cagcccctga ccgtcgcggc
     9721 gggagactcc ggaggctcgt tccacgacac ggtgtccctc gcgtccgtcg aagacgggga
     9781 tcacctgatc ctggtgagcg ccacggacaa ggccggcaac gtcacccgtc tggccattcc
     9841 cttccgaaag gatacaaccc ctccgactct gaccctggtg gctccgcgag cgcaggaccc
     9901 ggtgaacggg acgttgagcc tcgtgggggc cgtggaggat gagggccagg tggccctggt
     9961 ggagctcagc caggacggca agacgtttca gcaggtcgga cgcgagcgcc agttcaaagt
    10021 ggacgtgaac ctctccgcgc tcggggacaa ggcgaacgag ctgcagttcc gttgcaccga
    10081 cgcggctgga aacgtcgcgg tggttcgtcc ccagctcacg ctggaccttg ccgcggacgt
    10141 tcccgtatgc cagatccagc tgcccgctcc tggccaggtg atgcgaagcg acttcgagct
    10201 ctcgggaatg gcgttcgacg acgacggcgt cgcgtcgatc ctctaccgca ttgacggcgg
    10261 acccttcgtc acgctgccgg gaggaaacag ctaccgcgcc cagatctccc tggcggacgt
    10321 caccgacggt ccccataccg ttgacgtcaa ggctgtcgac atcggcggcc tggagagccc
    10381 gctcgtcagc tcccagttca tcgtctcgaa gtccgatccg gtctccacgc tggactcccc
    10441 ggacatcacc gcccacctgc gcggagaaat cgagctcaag ggaagcagcc atgaccctaa
    10501 cggcatcgcc accgtggaga tctccctgga caacggtcag agcttcctgc aggtggacgg
    10561 gaaggagtcc tggcgctatc gcctgaacac ggggatgctt tccgatggga cgcacgaggt
    10621 gatggtccgc gccgtggata ccacgggcgc ggtgggtctc tttgcgacga ccatcaacgt
    10681 ggacaatacg ccgcctaccc tggtggtgga ctccccgaaa gacggggatg tctacacgca
    10741 gaacctgacc ctgaacggac ggaccttcga caatctgggc ccggcatccc tggcggtcac
    10801 catggctccc ctggacggtc cggccaagga cggtccgaag gtcctggaag ccaagctcac
    10861 cgcgggagga atcctccgac aggaggtcga tctcaaagac gtgccccccg ggcggtacaa
    10921 catccagctc caggcatccg accaggccgg aaatcggacc gtggtttctc ggaacgtgac
    10981 ccgacaggcc agccaggagg cggagagcgt cgtgctctat ttccccaccg gaggggagag
    11041 ggtaagcgga ccgttcacgg tgagcggcag ggtcttctca cgtgccacca cggcggaggt
    11101 gaccgcgcgg gtcctggtcg acggccagct cctggaccag gttccactcc aggagcgtgg
    11161 cacattccac ctggaggtgg tggagggggc gctctccgaa agcagccata cgatcacggt
    11221 ggaggcacag ctacccggag gggaccgtct gctatcggat actcggctca tccgatacga
    11281 gacctccgga ccctgggtca agatcaccgc gcctacgcca ggagcgtaca tcacctcccg
    11341 cccgtatctc aaaggggagg ccggatggct ggaggccacg acaaccccgg atgccggcgc
    11401 tacccctggc gcttccgcca ccgctccttc gccctccagc ggctcggccg gcaaacccaa
    11461 ggtggatcct ggccatgcgc tcaaggtcgt cgaggtcagc atggacaatg gcagctcgtt
    11521 tgcgcgcgcc gagggcacgg ctagctggcg gttccgcctg gagacccagg acatgaccaa
    11581 cggggagctc cggctcctgg tacggtctac ctacgcgaac ggcgacacgg ccgtgacccg
    11641 gaccattctt accgtggata cccgtccgcc ccaggtgacg ctgctgcagc cctccgaagg
    11701 cgagcggttc aacgagaacg tggagctcgt gggtaccgcg tcggatgagt cgggcctcaa
    11761 ggacgtcggg gtgagcttcc gaacggggga caaggcgaac tacgaggtcc cctccttcat
    11821 ccaggggctc tatctggata cgcacctgct gggggcaacc tactgggacg ccgggatggg
    11881 cctcaccttc ttcagcgacg cggtcaagct gcaggcgcag gtcgggatgt ctccgccggg
    11941 ccggttctcc gggctggtca taggcgggaa gctcctggcc aacgtggcca cgctcccctt
    12001 cagttacctc ttcgggccgg actggagctt cttctccatg tccctggccg tgggcgcgga
    12061 cttttcctac ttcacgatgt ccggcgacac ggtcagcctg accgacaccg gcgcgatgct
    12121 tgccgggatc gtggcgcaac tggagttcgc caagttccgg attccgggat ggaccttcgc
    12181 caacacgtac tccctctaca cggagctgca ggtatggttc atctcttcgg acgtgcaggc
    12241 aacgaccgtt ccccgtctga gcttcggggt gagggtgggc ctcctgtaga tccgggacct
    12301 ccgggcggag gagccttccg cccggagggg cctcccgcgg atttcttgac gcaccgaggc
    12361 ggcgctggta cgctcctgag gcctctggag gtgcgtctcg tggattcctc atcccggtac
    12421 cgggttcttt cccccgagca ggaacgccag ttcctcgaga aggggtatct ggtggtagag
    12481 gggtgtctgg accccgagct tgcccgacgt tggaccgatc gtgcctacca gaggctggga
    12541 tgcagccggg acgatccaga gagctggccg cccggcatca cgtggatgga ccatgagacg
    12601 cggatgccgg tccgcaccgt atccccgcgc gcctgggcgg ccatctgtga cgtggcgggc
    12661 ggggaagagc ggctggagac caaggtcctg ggtctcccca actcggggca cttcaccacg
    12721 atgaactcgt tcgaatggtc cgatgccttc atcgtcaact tccgaaacgg ctccgagcta
    12781 ccctggatcc cgccgtcccc cgccctttcg ggctggcaca aggacggcag ctacttccgc
    12841 cacttcctgg acagccgcga gcagtccctc ctcaccgtcg tgctctggtc cgatgtccaa
    12901 ccgcagggcg gggggacctt cattgccccc gattccgtgc ggatcgtggc gcgctacctc
    12961 tacgagcacc ccgaaggggt ggagcccaac ggattcggac gcctcatcga ccagtgcaag
    13021 gagttcgtgg agatcagcgg gcccaccggc accttcgcca tcctgcaccc gttcatgctt
    13081 catgcctcct cgcgcaacca ttccggcaga ccgaggttca tgagcaatcc cccgatcatc
    13141 ctcaaggacc atcagaacct gaaccggtcc gaccctcggg agttctcgct cctggagcgc
    13201 gccacgctgc acgcgctcgg catggagcgt tgcgattttc ggcccaccgc gccgcgtcgt
    13261 tcagactggt gggtcgtggg ggagggctca tgaagatcct ttggggcgtc gtggcgtgcg
    13321 cgctggcatt gctctgcggc tgcgtcgaca ccaccacgaa catcacgctc aaaccggacg
    13381 ggagcggcac catcgacaag acgatcgtgc tcagcaagca cctcgccgag ttcgtcttga
    13441 agtcaggcat gagcggagac gccgcgtccg tggaacggag catgctcaac gagaaccagc
    13501 tcaagggcgc cgcgggtcag atggggaccg gcgtgagctg cgtgtcggtg gacaagatca
    13561 ccgcggagaa gggcaacggc tatcacgtcg tgtatgcgtt caaggacatc tccaaggtga
    13621 agatcaacac caacccggca tcggacctga acatgccggc cggcggcgcg gcggccgcac
    13681 ccgcccagta ccttacgttc aagttcgcct cgggatctcc cgccacgctc accgtcgttg
    13741 cccccaagcc ggccgacgcc aagcccacca cgcccgccag gcagatgagc caggccgacg
    13801 cggacaagat gtacgccacc atgaagccgc tgtacgcgga cctgcggatc ctgttcacca
    13861 tcaccgtggc gggaaagatc gcctcgacca acgccgcgta cgcggacggg tccacggtca
    13921 ccctcacgga catggacttc gccaagatcc tcgcggacga cgccacggtc aaaaagctga
    13981 gggccgcaca gagccagtcc atggaccagg taagggacat cgtgaagggc gtccccggcg
    14041 tcaagctgga gacccaggac acggtgacgg tgcgcttcga gtagacgcgg aggccgtccc
    14101 tatcggacgg tcgtcgcttc ccgttccagc ttgaggacca ggcggtccag agcgtccaac
    14161 cgcatggtct tggacttggt tcggatgctg tccaggctcg gcaggacggt catggcgtac
    14221 cggtggaccg tggcccgcgc cttctcggag taggtggccc cgccggcgtc cttgaggtag
    14281 gccatgacga gcttcttgcc cgaagtggtc agctcggact tgttgatgtg ctcgatcacg
    14341 gcgaaaaggg ccaggaggaa gctcatttcg ctcaggattt cctccttggg gattcgattc
    14401 atagcgtaac cgcaaggcgg atgacggtca agaaaccgct ctccttgact ccgcgacttg
    14461 attcctgtat gctcctatcc gtattccagc gggacagcgg cggcgaaaga gggcttccgg
    14521 cgcgtgtggc gtgagtcccg gtcagccgcc gaggagcgag tctgaaagcc cgaaaggtga
    14581 tgcatggatc gaatcatgac gttactgcct ctcctggacg ccggacgccc ctcccaggta
    14641 ccagaaactc gaacggctgc tcagccttct tgacggcacg ccgcctggcg tgcttccgcg
    14701 ccccgacgag tgagctcgca accgtagaat ctacaggagg accacgtggg tagatctccc
    14761 tatcaaggcg aaaaaagacg caaggagctc gctcgtctga agaagcagga agagaagaga
    14821 cagaagcgct tcaacaagaa acacggcacc tccgaatccg ctgagggagc cgggtcggaa
    14881 ggccagcccg tggatgcgcc ggaggccggt atagcccccg aaggtggctc ctcgccgtcc
    14941 gatggacggg actctacgcc gtcggatgga tcgccggtgg atcagacctc tcaaccggaa
    15001 ccggccccgc aggcgtccga cgagagtcag cgaggcggcc agcagtccta ggtaacgcca
    15061 agccgctatc ggggctgctc gagcgcttcg ataaccgcct gaatcgttcg atcatccgtc
    15121 aacatccacg catgggacag aaccggcagc acgacctccc ggccaaaggg catgcgggag
    15181 ctcgtaggtg gctcgatcgc cagatcgtaa gggctccaga tcgacgtaac ggggatcgaa
    15241 gcaagccgcc cctcgtccac agcgaggtcc gcgaggagcg tactgcccgg ccgcagctcc
    15301 gtcaccccgg agagtgactc cagatacgcg gtccgggtac cgtgattggg agtggagatc
    15361 aggaccagac gaaggatccg atccagcccg ttcatgcgct gcgcgtagta tcgcgcgatg
    15421 atcccaccca tgctgaaacc caccaggtcg aacggccgtt ggacgcccag cttctcctcg
    15481 ataagccgct cgagctctgc cgcggattgt tccatgcgga cggatccgtc gctgggatgc
    15541 agagacacgg cgaagacgga ccatcccgcg gcggagagcc gggcggccat gcggtggtag
    15601 gcctcagcgg aatcccagat cccgggcacc agcacgaccg cctcacccga tcccaagttc
    15661 gaggacggag ccggtgccga cacgcatgcc gacagcgcga ggctcagaac gagcaggccg
    15721 gctcgggagc cgcggcgcat ggtcctatcg gtcgaggaac ttcttgagcc gggcgagccc
    15781 atcccggatc agcggcgcgt cgattcccga gtacgccagc cggatgtaga actcccgttc
    15841 tccggggagc acgcggccga aatgggcgcg cgtgcagaag gagacgccgg tctcccgaag
    15901 caccgtctgg cgaaaccggg tgtagtccag gagcccgatc cggcgcatcg cgcccgtgac
    15961 gttgggaaag aggtagaagg tgacctccgg ccggaagcag cgtattcccg ggatggtgtt
    16021 caggatatcc acggccacgt ctctgcgctc ccgcaggatg ctcaagatcc gacgcgggcc
    16081 ctcctggttc ccccgaagcc ccgccagcgc gccgtactgg atgaaatggt tggagcagga
    16141 ttcgtcgttc acgttgagct tggcgatgac ctcgatgacc tcccgcgggc cgatggtggc
    16201 tcccagtcgc catccggtca tggcgaactt cttggagaag gtgtacagga tcacgcatcg
    16261 ctccgccatg ccgggcaggg acgccaggga gatcccggtc cccgagtagc gcatgtcgta
    16321 gtaggcctcg tcgcagagca cgtacaggcc gtgcttcagc accagctccg cgatgcgctc
    16381 gagctcgacg gggctgcact ccgaccccat ggggttatgg gggtcgttga gcaccagaag
    16441 ccgggtcttg ggcgtgatct gcttctcgat gctctggaag tccaggcgga agccgtcctt
    16501 cccttcgaca taccagtacg ggagcgcctt gccgcccagg aactcgatct gcgattcgta
    16561 gatgggatag ccggggtttg gatagaggac ttcgtcgccg gggttcatga gggccagaaa
    16621 gaacttgccg atggtgggct tgcctcccgg ctggatcgcc acgttctcca gggtgtaggg
    16681 cacgtggtgg gaggcagcga cgtcggcggc aaccgcctcg cgaagctgcg ggatgccggc
    16741 ggttgggcag tagccggtct tgccgtcccg catggccttg accgaagcgt ccatgacgtc
    16801 ctgcggcgtg gggaggttca ggtccccgag gtggaaggga tagaccgtgt tgcccttggc
    16861 cgcgaactcg gccgcctccg cggacacggc aaacgccgtc tcgctgccca gccgtccaat
    16921 gcgttccgct atcgtcatgt gtcgtgctcc tgcgccccgc gggcagggtc cgcggggccg
    16981 atcggcgtat ccgctggcct ggactccgtg tccacccgcc ccctcagaaa gagcttgacc
    17041 ttttccgggg gtacgggtat tctacctccg agacttctta aagaaggctt cgagaggtct
    17101 acggtaacac aaaaaagaag ccccgtcatt cggacctttc ttttaccacc ccgagggaca
    17161 atcgaccgga cagcggcgtc caggaacacc cgccatgttc tgctcctcct atggtgcgta
    17221 gcaaagggcg atgatgacag atctccaagg aggagacagg atgaacaaga agctttacgt
    17281 tggcggcctc gcctacagcg tgaacgacga ccagttgcgg cagctcttcg cgagccacgg
    17341 gagcgtgcag tccgccaagg tcatcacgga ccgtgacacg gaccgttccc gcgggttcgg
    17401 tttcgtagaa atgggcaccc aggaggaggc cgagaaggcg atcgccgccc tcaacgggac
    17461 ccagttcgaa ggccggaccc tgacggtgaa catctcgcgc ccgcgtgagg agcgcccggc
    17521 ccgcagcggc ggccgcgacc gctactagcc ggcaacgcgg cgacacaggt cgccggaaaa
    17581 aggagggggg caggtcggtc cccctttttt ttgcccgggg ctctacgccg ccccttgggc
    17641 ggggctctac gccgcctggc cttccaccat ggagaggaag tactggtgca gccggctgtc
    17701 tcccgtcagc tccgggtgga acgctgcggc caggagtcgc ccctggcgcg ccgcaacgat
    17761 gctgccgttc ggcaggctgg ccagcacccg gaccgacggc ccgcagccaa cgatggccgg
    17821 cgcgcggatg aatacggctc tcaggggcgg accgccgatc cccgacaccg cgacgtccgt
    17881 ctcgaaggac tggatctgcc gaccgtaggc gttccgtcgt atgctgaggt ccatcactcc
    17941 tagccgtggc tgggtggagc ccccgaggtc gcgcgccagg aggatggctc ccgcgcaggt
    18001 cccgaacacg gccaggccgg accggacccg ggctctcagc ggctccacca gcccatagtc
    18061 caccatgagc ttgccgatgg tggtgctctc cccaccggga aggatgagcc cgtccagcga
    18121 gtcgagctgc tgtggcagct tcacctctac cgcaaccacg gatccccagt cggcggtccg
    18181 ctgcgtggcg aggctcccga gcatggccag gtgctcggca aagtctccat gcaaggcaag
    18241 cactccgatg cgcacgatca cccgccccga acggccagaa gcttgtccgg cgcgagcgta
    18301 gagatctcca cccccgccat cgcctctccc agacccttgg acacctccgc cagaatggcg
    18361 ggatcctgga agtgggtcac ggcccgtacg atggccgctg cccggcgagc gggattctcc
    18421 gacttgaaga tccccgaccc gacgaacacg ccgtcggcgc ccaggtgcat catcagggcg
    18481 gcgtccgccg gcgtggcaac gccgccggcc gcgaagttca cgaccggcaa gcggccttct
    18541 tgcgcgacga gcaccaccag atcatacgag gcgttgagcg ccttggcctc cgccatgagc
    18601 tcctcggcgc cccgggagcg caggtggcga agcgcgccct gcaccgcgcg catatggcgc
    18661 accgcctcca cgacgttgcc ggtgccggcc tcccccttgg tgcggatcat ggccgctccc
    18721 tccgcgatac gccgcagcgc ttcccccagg tcgcggcatc cacatacgaa cggcacctgg
    18781 aacgcatgct tgtccacgtg gtgggcgtcg tcggcggggg taagcacctc ggactcgtcg
    18841 atgtagtcga tgccgatggc ctggaggatc tgcgcctcca ccgtgtggcc gatgcggcac
    18901 ttggccatga ccgggatggt cactgcctcc atgatctgga ggatcatttc ggggtcgctc
    18961 atccgagcca cgccaccggc ggcgcggatg tcggccggga cgcgctccag cgccatgacg
    19021 gcgcaggccc ccgcctcttc cgcgatccgc gcgtgctcgg gggtgacgac gtccatgatg
    19081 accccgccct tcagcatctg ggccaggccc ttcttggtga tccaggttga ctgctccata
    19141 ccgactcctt ggtcccaaga tcgcccagcc agtggtataa tcaaagagcc atttttctta
    19201 tgatcgattg ggccactttc cgacccgcga tcggtatcgg ggggttgcat gcgcgccgac
    19261 gacctcatga tcaggctgac gccggccccg gggcggggaa cgccgacgac ccggccgcgc
    19321 tctacgctgg gcagcgcggc cgttccgcgc aggccatacc atcgccagat cttcgacggc
    19381 atccgcgagg ccatcctggc cggtcggctg cgcccggccg accgcctccc cgccacgcgg
    19441 gagctcgcga acgagcttgg tgtggcacgc aacacggctg cccgggcgta tgaggacctg
    19501 ctggcggccg gctacctgga ggcccgcgta ggcgccggca cctacgtgtc ctcggggatc
    19561 cgtccacccg tagggcgggg ctctacgcca cccgtagggc ggggctctac gccgctcctg
    19621 gggcccccgg cgccccgtag cgcgcaggta cctgcgcggg cggcggaccc gctacggttc
    19681 gacttcctgc cgggcatccc ggactgggac gcgttcccgc gggcgttgtg gcatcggctt
    19741 ctggggcgcg cgctacgggc gcggccgtcg gagctccgcc gttacggaga ccccgcgggc
    19801 tatttcccgt tgcgcgaggc cattgcgcgt taccttgcgg tctcccgcgg gaccgtcgcc
    19861 acgcccgaac aggtggtcat cgtgagcggg tcccagcagg ccctggacct catcgccagg
    19921 cgggtcctga gcggaaggga tacggctgcc atcgaagacc ccggctaccc cgaggctcgt
    19981 cggctcctct ccgcggcagc ggcgcgtctc tgcaccgtgc ccgtagatga ccagggcatg
    20041 gtcgtcgaga ggctggcgac cgccgcgggg tcggacccca ggctggtcta cctgacgccg
    20101 tctcaccagt tccccaccgg cgccacgctc tcgctggcgc ggcggctggc gttgttggac
    20161 tgggcgggca gacgggacgc gctgatcgtc gaagacgact atgacagcga gttccgcgcc
    20221 cccggccgaa ccatcgagtc tctccagggc ctggatcgcg gccaccgcgt catctacatc
    20281 ggcacgttct ccacggtgct ctttcctccg ctgcgggtgg gatacgtcgt tctgccccca
    20341 tccatggtgg agccgttcgt cctcacgaag tggcaggcgg atcgacagac ccccacgctg
    20401 gagcagatcg cccttactga cttcatcttg gagggccatt tcgagaggca cctccgacgc
    20461 atgcgtcggc tggtcgccgc gcgacgccgg gccatgctcg cggccatcac ggaccgcctg
    20521 ggggacgtgg aggtcgccgg cgccgactcg gggatgcacc tcatgatgag gcttccacca
    20581 ttgacgggcc acgccgcgcg ggaggtggag gagcgggtct cggcggcagc ggcggagctg
    20641 agcatcgcgg tctatcccgt ggcgccctgt cgatcgggtc ccgtgcggca ggcggcgttc
    20701 gctctgggct acgccgcggt gtccgaggag atcatccgag aagggatctc cgcgttcgcg
    20761 ggggccatcc gccgggtcta tcgagacgga caccgctgat cgaccgagcg tggttccgca
    20821 gggcaggccc gcgccgccct ggttcgccct caccgcggtc cggagccagg gtcgtcagcg
    20881 gatcagttca ccacgttctg cggattgccg ctgagaaagg cacggacgtt tgccgcggtg
    20941 gcagccatga ggcgcctccg tgcctccagg ctggcccagg cgatgtgcgg ggtgaggagg
    21001 cagtgcttcg ccgtcaggag cgggttgtcg ttgcggatcg gctcctggga caccacgtcc
    21061 agagcggcac cggccaggtg cccctggttg agggcctccg cgagatccgc ctcgttcacc
    21121 agtccaccgc gagccgcgtt gatgagaaag gcggtgggct ttgcgtggct gaggaggctg
    21181 cgatccacga accgggcgtt cgaagcgttg agggagctat tgaggctcag gacgtcggac
    21241 tgctgaaaga ggtcctcggc tcgatctgcc cacgagaacg gctggtactc cgggggccgc
    21301 tctcggaagg ggtccagagc cacgaccttc atgccgaatc cgtgggcgat ctcccccacc
    21361 cgcctgccga tcctcccgaa gcccacgatc cccatggtct tgccggcgag ctcgaccagc
    21421 ggctgcacgg tgaccgagaa atccccgctg gccgtccacc gcccgtcgcg cgcggcccgg
    21481 ttgtgctccc cgaccccgct ggccagctcc aggagaagag cgaacacgaa ctgggctacg
    21541 gactccgtcc cgtactccgg gacgttggca accggaatcc cgcgcgcgcg ggccgctttg
    21601 acgtccacga cgtcgtaccc cgtagcaagg acggacacga accgaagccg gtccagggcc
    21661 tcaatggagc gctgcgacaa cggcgtcttg ttggtgagca ccacctcagc cgagccggcc
    21721 cgtcggacca cttcggcttc cggagttcga tcgtgaacgg tgatctcgcc gagcgccgag
    21781 accggatccc atggattgtc tccagggttc aacgcgtacc catccaggac gacgattcta
    21841 accttcgagt tcatctgcac cgcctcaccg gcacaaagat ggaccaaaag ccgctgctcc
    21901 ggcaacggcg cctcacaacg atagcggaac gcccgagcag gcaacgaggt agcctgcgat
    21961 ggttgcttga ctcacgtctg agcggtcatt acgcttttgc aggttataac gtacaggagc
    22021 ctgaggtgac tgcaaacaca ctgaggattc agaagctgag tctggtaggg ggaaggctat
    22081 gtctcgactt caccaatacg gtgagctggc gaggcgccga gaaccccagg gacctcttgc
    22141 agagttacga ggacctcgtg acctggggcg ttcgagccga tcttctcgac tccaccagcg
    22201 caggcgccct gctggcgcgt gccagctcgg aggcacaagc cgcggaactg gcgctggcca
    22261 aggcaaaggc cctccgggaa tccatcgccg gaatctacga tgccgttgcg agcggcggga
    22321 accccaaaga cgacgacctg tcgcgcctga caacctttgt gcgggatgca ttctcccgcg
    22381 tccagctctg ccgctcggag agcggctacg acattcgctt cgagcgggct ggcgccttgg
    22441 atcaactcct tccgcccata gcatggtccg ccgcggagct gctacgggac ccggcgctga
    22501 cccgcgtcaa gtgctgcagc ggaaggacgt gcggatggct cttcgtggac tccagcaaga
    22561 accatagccg tcgatggtgc gagatgaagg attgcgggaa ccgcgagaaa gcgcgacggc
    22621 actatcacaa gacccgaatc cagggaaccg gggccacgta gcgacggtcc ttgctcgtcc
    22681 gtcccctcgg cgctatgatc gcccaggtct gacatgtcgt ttctcatcac cggatactcc
    22741 gtggctctgt ggggttcggc ggcagtctcc accgtgaccg cggccgtggc gtatcggcgg
    22801 agggccgcgc cgggcggccg ggcactggcc ttgatgttgg tctgcgtggc cgtctggggc
    22861 tcgatggccg gcttcgagtc cgcggcggtc gggcttcccc tcaagatccg gttctctcag
    22921 ctcacgtatg tcggggtcgt cggcgtggcg cccctctttc tcctcttttc cgtcgcctac
    22981 accggcagaa agcccttccg cctcggcatc agcctgctgc tctgggtcgt gccgctcgtc
    23041 accgtggcgc tcgcgttcac gaacggttcg cacgggctgg tctggaagag cttcacggcg
    23101 gacccatccc cgcagaggaa cctggtctac tacggccacg gaccctggta ctgggtctcg
    23161 ttggggtact acgtcgcgct ggtgctcggg gcaacggtca tcatattgca tgcttcgatt
    23221 cgagcgccgc ggatctacct tcgccagacc ctggtcatga tcgcgggagt cgttgtcccc
    23281 tggctgggcg aggtgctcta cgtctcaccc cgcaacccgt tccccggtct cgacctcacc
    23341 cagatcgggt tcgccgtcac cggcatcctt ctcctgtacg gcttctcgcg gctgcgtctc
    23401 ttcgacgtgg tccctatggc gcggtccctg ctcgtggaac ggatgcgggg cggcctggtg
    23461 gtcctcgacc tgaagggccg gctcgtggat ctgaacgccg ccgctcgaca gatgctgcgg
    23521 gcggaagacg ccgcggtagg gaaaccggcg gccctggcgc tgccggctct tgcggcgcat
    23581 ctgccggcgc cgggcacggc cccggaggac cagacccgcg agatccccat gcccgggacg
    23641 gaggatcgac ggctcgagct cgccgtctcc tccctgcggg atccgatggg gaggtccggc
    23701 ggagtcctga tcgttctcca ggatgtaacg gcccgtcacc ggatggagca ggagagggag
    23761 aagctcatcg gagagctcac caaggctctg gcggacgtgc ggaccttgca gggcctcctg
    23821 ccgatctgcg cctcgtgcgg gaaaatccgt aatgaccggg gctactggga gaacatcgag
    23881 cactacctcg ctgcccacac cggcgcgcaa ttcacccacg gcctgtgcga ggaatgcatc
    23941 cacatgctct accccgagct ggatgagcct ccgcccagtg ccgacgcgcc tccctagtgc
    24001 cctttcaggg tccgggcctt catgaggtcc ggataggtct tctgcggatc gccgtaccgg
    24061 ttgatgccat agtctttccc ccgggcatga tagagctggt gccgcgccag gttctccggg
    24121 gtcagacgcg tggaggacgc gatgtatcgc atcgtgtacc cggtccgcct gcgcgtgctg
    24181 gtgttggccg ggctgccgtg gatcagcttg gcgtggtgga ggctggcctc gttctccgcg
    24241 agcacgatgg gcacggcctt cttctcgtcg aactggccct tgcggatctc cgtcatgaac
    24301 aggttcttgt tggggtccac gttgtagtac tgggagaagc cgttgtcgtg ggtgcggggg
    24361 atcacgtaca tgcacccgtt gccctcatcc gagctgtcca acgcaagcca gacggtgacc
    24421 acctccatgg gggtgagcat gtcccgccag tacgcggagt cctcgtgcca ggggaccctc
    24481 aggccgtcgc cggccgactt ggagatgaag tggctggaga acaggtcgat gtccggaccc
    24541 aggatgtcct ctaccaggtc caggacctca tcgtcgaaca gccattcgaa gagcgcggtg
    24601 tccaggaagt gcgggacgtc catgtgctct cctcgcttgc cggggtccag ggccgcgagc
    24661 ttcttctcga aatggtcctt cagggagcgg aaccgggcgt cggtaaggac cgggtgtcga
    24721 tagacaaggt aaccttccgt cttgtaacgc gcggtttcct ccgcggtgag cttcttcagg
    24781 gccgtcatga gcacctccac ggaccaaggt actgccgccg cctgccgcgg tgaagcgtgg
    24841 ggttgggacg tgtcgaagac cggtcaaccc gagggttctg gagagaggct gggtcggctc
    24901 aggagtcctt gagaagccaa tcgaggaagt ccttgacctg gcgatcccag tagccccact
    24961 cgtggtcccc cggcccgtcg ctgaagctca ggtccaaccc gaggcgccga gcatggtcgc
    25021 ggaagaggag gttgtcctgg tacaggaagt cttcggttcc gcagcactgg tacagtcgca
    25081 gccgggctcg ggccgccggc gggagcgcat cggccagatg gaagagatcc cgatccgacc
    25141 cggcaacgcc gcccgcgtct ccgaagatcc gttgccactc ggccttccgt tcttcatccg
    25201 attcctcgac gacttttgcc atgttcagcg ccccggagaa gctgcctgcg gcgcagaaaa
    25261 gatccggcct gcgcaaggcc agccggaagg cgccgtatcc acccatcgag agcccggcca
    25321 cgaaggtatc ttcgcgcctc ggagacaacg gaaagaggga tcgagtgacg gacggaagct
    25381 cctcttcgaa gaaggccccg tagcgagctc cgttcgccat gtcgacgtag aagctgcgtc
    25441 ccgcggcggg catcaccacc gccaccccgt gtgcgtccgc gtaccgctcg atcgaggtcc
    25501 gacgcgccca gatggtgtga tcgtccgaga gcccgtgcag gagatagagg gtacggtaag
    25561 gccgcttcac tccctccccc aggggaagga cgacgttcat cgacgtcgag agggcgagcg
    25621 tggcgctgaa gaagtcgcac tggaggaagg ccatggccaa gcctaccgga aacgcggccc
    25681 ggcgcgcaat gcccgtggta gaatggccgt cgctggaggg atgcatgcga atcacccgga
    25741 ccatcgttgc gtgcgtgtgc gggctcctca tcctggtcgt agccgcgctg gtggggtcca
    25801 tcgcgttcat gcggtccacc cgccgcgcgg acttcaccca ccagcgggac cggatcgagg
    25861 cgctggcatc cgcgcggccg gacgcccttg cccccgccgg gccggagacg ccgggccccg
    25921 gcgcagggcc agcgaaagcc ggcgacacac cggcggtgga ccgcgcggtt cgagacgacc
    25981 tgcggctcaa ccagatccgg atggtcgcga ctcataacag ctaccacgtg cagtcggacc
    26041 cgcttcgcct gttcctcatc gggttgttcg agccggtgtg gcccgcccgg cttcgctaca
    26101 gccatgcccc gctcagcgag cagttcgatc atggcgtgcg cagcgtcgag ctggacgtcc
    26161 gcaaccaggg ttcgaggttc gtcatcgccc acgttcccct ggtggatgac cgcgccacct
    26221 gccccgactt cctcctggcg cttcgggaga tccggctctg gtcggagcga catcccgacc
    26281 atctccccat cgtcgtgctt ctcgagcttc tcaacgactg gagcttcctg gacccgcggc
    26341 ttcgagactt cgatcgaccg gcgctggatc ggctggacgc ggagatccac gacgcctttc
    26401 cggctccgga gctcctgacc ccggacaacg ttcgcgctgg cgccgtgacc ctggaagccg
    26461 cggtggtggg tctgggctgg ccgcggatcg gagagatccg gggccgcgtg atgttcgtca
    26521 tgcacgacaa cgagacctac cgccggttgt tcgtggaggg caacccgacg ctggcaggac
    26581 gatcgctctt tacctgcgcg ccatcaggca acccggatgc ggccgtccgc gtcctggacg
    26641 atcctgtcgc ggacggaccg accatcgcgt ctctgaccca cacgggatac ctggtgcgca
    26701 cccgcgcgga cgcggatctg gacatcgagc ctgccgtggc tcaggcggcg gtggcgagcg
    26761 gcgcgcagat cgtctccacg gactacccgc cgtccgagcc ccgtaccgac acgggttacc
    26821 ggttcagctt cccgggcggg cgcatggtga cggcccgcat gggaccatga cctcgccacc
    26881 gggtgccgat cccggccttc gtgatccgca cgatgaccac gggccctggg cggccgtccc
    26941 ggtttggaat cgcccgtagc ggatgctaca ctttgcgtcg ggtctccccg cgtcacgcct
    27001 cgcgggagaa acccgatagc gtatggtgaa catgagcaat tcgtttagcg cgccggctcc
    27061 ggcctgcagg gtcctggtgg tcgacgacga gaagtggatc cgacacgctc tgtgggtcta
    27121 tctctcgcgg gacggctctg tcgttgaaac ggcccgaagc gccgtcgcgg ccctgacgtt
    27181 ggcatcccga aggaactacg acgtgctcct ggtggacctg gagatgccgg gcacggacgg
    27241 cctctctctc cttggtgagc tgacgaggtc ctgcggccag gcgctgtgca tcgcccttgc
    27301 cgcttctccc gaggagccga ggcccgcgga ggcgttgaag ctgggcgccg cctcgtgcct
    27361 gctcaagccg ctggatgggg aggcccttgc cgagattgcc cgatggattg cggtccgcaa
    27421 cggcctcacc cgagcgcctc tcggccaccg ggtgacggat ccggagcggg aggtcgttgc
    27481 cgagagtccg acctcccagg cggcgttcct ccaggtcagc cgagccgcac cgctttccgg
    27541 gcccatcctt ctggaagggg agagcggctg cggaaggggc ttcctggcgc gtctggctca
    27601 cctgtggggt cccggctcct cgggtcgact ggtccggttg gattgcccga gggcctcgga
    27661 aagcctgctg gaaagcgcgc tggagagcgc ccgtggcggc ggcctctacc tggaggagat
    27721 cgggaagctg cctcgcgtgc ttcaaggaag actggccgcg gcgctcgccg accgggatgg
    27781 ccgtggcggg aggcacacgc gcctcatcgc ttcgacgtcg caggacctga gacgggaaac
    27841 ccgcttggga cggttccacg gggagctcta tcggcgcctc gacgagacac ggatccgtgt
    27901 accccggctt cgggatcggc ctcaggacat ccttcccctg gcgtgccggt ttctgcggca
    27961 atcggacctt cgttcgggta agcccgcgca cggcatatcc gcggatgccg tgaggttcct
    28021 gcagacgcag cggtggcgcg ggaacctcca cgagctcatc gacgcggtgg cttgcgccgc
    28081 cgcctgtgcc gaaggggtcg atgtcacggc tcgggatctc cgtgcatacc gcgcgcggtc
    28141 gaatctcctc tggcgccctt acctcgcgaa cgcccgaaac gggttgtccc gagtgatccg
    28201 acggatggtc ggttcgggaa ctccggagga ggccaaagcc ggcaggaagg cctgcaccag
    28261 gtacgcgtag gacttctgct ccccgccccc gggcagggcg gggttgaacc agccccggtc
    28321 ctggctcagg agaactcgat cctcgaatcc ggcgtccaac aggcgatgga tccgatcgac
    28381 gaaccctcca tcggtttgcg ggttcccgat ggcgtcgtac tcgatccagg ctccctgtcg
    28441 ggccgccgcc agattcgtct ggaagtcttg ctcgacgtgc gcat
//
LOCUS       DASRZS010000280         3135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4389421, whole genome shotgun sequence.
ACCESSION   DASRZS010000280 DASRZS010000000
VERSION     DASRZS010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4389421"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VFH83_06965"
     CDS             complement(<1..936)
                     /locus_tag="VFH83_06965"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET6486142.1"
                     /translation="MKNFELFLPTRYLFGTGVVQKAGAETGKLGRRALLVTGKSSASR
                     SGLLATVENLLKAEGLFVCSLAGVDPNPRLSTCVAGARLCKEHAVDVVVAVGGGSVLD
                     ASKTIAALALDSGEPWDFFTGARPITRALPLVDVLTVAATGSEYGPYAVITNEKTGEK
                     LGTFGEAIIPRVSIVDPSLTFTVPARHTAFGAVDIISHHLEAYLACEEAPPIQDELTE
                     AVIRSVIVNTGKALTNPRDLEARSCLLWANTLACGVFMVGRGAMRFDAHAVEHELSAA
                     YDVPHGAGLAVILPALMRWRVEKDPTRVERFARQVF"
     gene            1129..2109
                     /locus_tag="VFH83_06970"
     CDS             1129..2109
                     /locus_tag="VFH83_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486143.1"
                     /translation="MASVQTIRGPIDSKQLGVTLIHEHVSFGAVSRQQIDGPYIDKAF
                     DFNLDLLKKARDVGINTIVELTPWPNVRKIVELNDRLPELNFILCTGAYLETSHHPIV
                     KMGETEFHDHMVRNITRGYEGFESTGVKAGIIKVAGNLSKLTEWEKRNFRLAARVQRE
                     CRVPIATHACAGCREQMTVLKEAGANIAATFYSHVEAEFGWDGRTVPQEAEYLADVAR
                     AGGYLHFNNFDFEFDTPFPDMMYLINHLETHGFGEKIFFSIDTNIEIDDDGKIWMEAE
                     KKHPETGKRNYAYAITNAVPMLMSAGVSLQRITKYLVDNPRRYFEAAALS"
     gene            complement(2102..>3135)
                     /locus_tag="VFH83_06975"
     CDS             complement(2102..>3135)
                     /locus_tag="VFH83_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6486144.1"
                     /translation="ERILAQIGHERTDFVPYGGFGLDDELELRITGKLGGQRWLAEVH
                     GHDHIHNINHANAVFLDDWAAVKVGAEHVDRFGTTWRVDHRAYHITRPALIEASLSGY
                     RFPVAPQFLPDGWRWAAQGEIERERDRFLILCLNGIFEGSWGIRGFESLLEDFLTSPE
                     FAEELLEGMTNLLLGLLDTYLELPIDGILMYDDWGGQRGSLFGRERWVRFLKPRVQRV
                     YGRIHAAGKIAIAHCCGNITELIPDLIDAGLDVLESVQPEAMDPYALKREFGKDLTFW
                     GGLGTQGLIPFGAPDQIRQEVRRLCAEMGEGGGYILAPAKALQPETPTANALAVIEAF
                     LEEAGVQLS"
BASE COUNT          592 a         1008 c         1011 g          524 t
ORIGIN      
        1 gaatacctgc cgcgcgaacc gctccacgcg ggtggggtcc ttctccacgc gccatcgcat
       61 cagggccgga aggatgacgg ccaatccggc gccatgtggc acgtcgtagg cggcgctgag
      121 ctcgtgctcg acggcgtggg catcgaagcg catggccccg cgccccacca tgaacacgcc
      181 gcaggccagc gtgtttgccc agagcagaca ggagcgcgcc tccaggtccc tgggattggt
      241 cagagccttc cccgtattga cgatcacgga tcgaatcacc gcctcggtga gctcgtcctg
      301 gatcgggggc gcctcctcac aggccaggta ggcctccagg tggtgggaga tgatgtcgac
      361 ggctccgaag gccgtgtgcc gcgccggcac cgtgaacgtc agggaaggat ccacgatcga
      421 gacccggggg atgatggcct cgccgaacgt gccgagcttc tccccggtct tctcgttggt
      481 gatcacggcg tacggcccgt attcggagcc ggtcgcggca accgtgagca cgtccaccag
      541 gggcagcgcc cgggtgatgg ggcgggcgcc ggtaaagaag tcccaaggct caccgctgtc
      601 cagggccagg gccgcgatgg tcttggaggc gtcgagcacg ctcccgccac ccacggcaac
      661 gaccacgtcc acggcgtgtt ccttacagag gcgggcgccc gcaacgcagg tggaaagccg
      721 aggattggga tccaccccgg cgagcgagca gacgaacagc ccttcggctt tcagcagatt
      781 ctccacggta gccagcagtc cgctgcggga cgcgcttgac tttccggtga ccaggagcgc
      841 ccgccgcccg agcttgcctg tttcggctcc cgccttctgt acgacgccgg ttccgaacag
      901 gtagcgtgtg ggcaggaaaa gctcgaagtt cttcatgctc gcatctccgg gatcgccatg
      961 gatgccacag tttagcagac atgagtgaga ttgtaaagag aacggggtac aaacttgaca
     1021 aactgggggc tgacctgata cggtttcata gaagtaagcg ccgatcgtgt ttcattaacc
     1081 attgttcctt ccagaatcga aggaaccagg cagaagggag agagcgccat ggcgtcggtt
     1141 cagacgattc gcggcccgat cgactctaag cagcttgggg ttaccctgat tcacgagcac
     1201 gtctctttcg gggcggtttc ccggcagcag atcgatggcc cctacatcga caaggctttt
     1261 gacttcaacc tggacctgct gaagaaggcc cgggacgtcg ggatcaacac cattgtcgag
     1321 ctgaccccct ggcccaatgt gcgcaagatc gtcgagctga acgaccggct tcccgagctc
     1381 aacttcatcc tgtgcacggg ggcatacctg gagacgtcgc accatcccat cgtcaagatg
     1441 ggagagaccg agttccacga ccatatggtg cggaacatca cgcgaggata tgagggcttc
     1501 gagtcgacgg gcgtgaaggc cggcatcatc aaggtggcgg gaaacctctc caagctgacg
     1561 gaatgggaga agcgcaactt ccggctggcg gcccgggtgc agcgggagtg cagggtcccc
     1621 atcgccacgc atgcctgcgc cgggtgtcgg gagcagatga ccgtcctcaa ggaggctggc
     1681 gccaatatcg ccgcgacgtt ctactcccac gtcgaagcgg agttcggatg ggacgggcgg
     1741 acggtgccgc aggaagcgga gtacctggcc gacgtggcgc gggcgggcgg atacctgcac
     1801 ttcaacaact tcgacttcga gttcgacacc ccgttccccg acatgatgta cctgatcaac
     1861 cacctggaga cccacgggtt cggagagaag atcttcttca gcatcgacac caacatcgag
     1921 atcgacgacg acggcaagat ctggatggag gcggagaaga agcaccccga gacgggcaag
     1981 cggaactacg cgtatgccat cacgaacgcc gtgcccatgc tgatgtccgc gggggtctct
     2041 ctccagcgga tcacgaagta cctggtggac aacccgcgca ggtacttcga ggccgcggcg
     2101 ctcagctaag ctgaacgcct gcctcttcca ggaacgcctc gatgaccgcc agggcgttcg
     2161 ccgtcggagt ctccggctgc agcgccttgg ccggcgccag gatgtagcct ccgccctcgc
     2221 ccatctcggc gcacagacgc ctcacctcct ggcggatctg gtccggagcc ccgaagggga
     2281 tcagaccctg agtgcccagc ccgccccaga acgtgaggtc cttgccgaac tcacgcttca
     2341 gcgcgtatgg gtccatggcc tcgggctgca cgctctccag gacgtcgagg ccggcatcga
     2401 tcaggtccgg aatcagctcc gtgatgttgc cgcagcaatg ggctatggcg atcttgccgg
     2461 ccgcgtggat ccggccgtag acgcgctgga cgcgaggctt gaggaagcga acccaccgct
     2521 ctctgccgaa gaggcttccc cgctgtccac cccagtcgtc gtacatcagg atgccgtcga
     2581 tgggcagctc gaggtaggtg tccagcagcc ccaggagcag gttggtcatg ccttccagga
     2641 gctcctcggc gaactccggg ctggtgagga aatcctccag caggctttcg aacccacgga
     2701 tgccccaaga gccctcgaag atgccgttga gacagaggat gaggaaccga tcgcgctccc
     2761 gctcgatctc cccctgagcg gcccatcgcc aaccgtccgg caggaactgc ggcgccaccg
     2821 ggaaccgata gccggacagg gacgcctcga tcaacgcggg gcgcgtgatg tggtaggcgc
     2881 gatggtccac gcgccaggtg gtgccgaacc ggtccacgtg ctccgcaccg accttgaccg
     2941 cggcccagtc gtccaggaag accgcgttgg cgtggttgat gttgtggatg tggtcgtggc
     3001 cgtggacctc cgccagccac cgctgtcctc cgagcttgcc ggtgatccgc agctccagct
     3061 cgtcgtccag accgaatccc ccgtagggga cgaagtcggt ccgctcgtgc ccgatctgcg
     3121 cgaggatccg ctctc
//
LOCUS       DASRZS010000281         8140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1311765, whole genome shotgun sequence.
ACCESSION   DASRZS010000281 DASRZS010000000
VERSION     DASRZS010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311765"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VFH83_06980"
     CDS             complement(<1..861)
                     /locus_tag="VFH83_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HET6486145.1"
                     /translation="MRVRCWLQDSLLRIYPTGNRPADQADGQPWLEAGRGERLSFQLA
                     IESLGRGDPARVSVSAAAPPGIAARVRRLGFVPVPYHNTETPDGELDSMDHIPGYVPD
                     PLFEESQTLLPAGQTAAFWITVRVDRDAKPGRFSIPLSVLCQGESVAELTAEVIVHEA
                     RIAPRKDFPVTHWFYADAILDWYRLSGFEERFWTLLRQYLVNVAEHGQDTIYVPALTP
                     PLDGVKRPTQLVAVQAAGERYAFDWSLVQRYVRLARDCGIDRFEWPHLFTQWGVANAI
                     RVYHGRGEDER"
     gene            complement(872..1777)
                     /locus_tag="VFH83_06985"
     CDS             complement(872..1777)
                     /locus_tag="VFH83_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486146.1"
                     /translation="MTAERERRPEARTRRRWGDRYDGRRVRSLNPLYQMTPYIMRSRV
                     DAQVYFEDQVEIGPAERWLRQMRDQGSAKLGLLHVLIAASVRTLSQKPRLNRFVAGQR
                     IFARNEILISLALKKKLQEDSPETTIKLAFLPTDTIFDVADRINAAVAENKDLETQNE
                     TDATARLFMMAPGFLVRFLVWLFRTLDFYGKLPRAIHRASPFHTSIFVTDLGSLGIRP
                     IYHHLYEFGTTSVFVAFGIKERRHVTDRNGAIVERRSVGIKVVGDERICDGHYFASAF
                     RYLTTLLRDPRQLESPPERVVDDEE"
     gene            1882..2241
                     /locus_tag="VFH83_06990"
     CDS             1882..2241
                     /locus_tag="VFH83_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HET6486147.1"
                     /translation="MDFKRFFTPEEAERALPLVRQIVEDILGTARRIRELPEEDSAAR
                     RLEAELQEHLLEIQELGCFFKDWNFSVGLVDFPAIIDGETVFLCWRSDERSLEWYHGI
                     DDGYQGRRRLPGVAAAN"
     gene            2269..4539
                     /locus_tag="VFH83_06995"
     CDS             2269..4539
                     /locus_tag="VFH83_06995"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET6486148.1"
                     /translation="MRTRFTLNGSWRMLIGERHLRAVDVPSSYRPVGVATLERELPAV
                     APAKGRRVYLCFDGVANTGEVAVDGTHLGPMLPFVPYTFDITRHGAPAHLVRVTLTDL
                     GATFGYSPGWESYGGIIRDAFLVVADEAAFSDVYVQTWVASDLETARCAVRVEYRNFA
                     STAVRGTLRAELTLEGDRVAAEPEPRADLLMPPDGQNGVTTLEFGVSGIRLWSPERPT
                     LYRLTLSLEDEDGRRIDTEEVSVGFRDFRVDGRRFLLNGRPIFLKGVCRHDLWQDRGY
                     TLTPAQMEQDILAIKRLGANYIRLVHYPHHRGIIDLAEKHGILVTEEPGLWNIQVFAK
                     DRTRLEQHKRDSLEVMERTIRRDRGSPAVIAWLLGNECHMEDLGLEFLKRAAALCRSL
                     DSRRPVSFSHLYTKDLADGKKPYAEAGLDFYDYHPYTPSVETFAGVMKVFDDKPLVFG
                     EWGGWFVRDSEGLLRMFGDAFARASAFGSSDALHLNGIAYWEWADMRQYGRGYPGCEA
                     GILTEGLVDEARNPKPDYQVMKDIFTAIDAGGPLPSRAPLTPRVTMPASLDGYAPAGK
                     LVCIDLGPVVEEAAQRKAWESLEPGHRNPFPVVPAIGPIPFRPSGADGRPVVLGSLAP
                     AAVIAVGAPVRAFHALGHVAVHGHPLVGSVGQRVAQYIIRYDDGAEETVDLLNAVHLA
                     RQNAVYEGTRIEPVAFDAPVVLREVRDPDYRHLELRYYRLVPGRPGALIRSLRFATDS
                     GDEFRPLLYALTVERG"
     gene            complement(4572..7577)
                     /gene="pgmB"
                     /locus_tag="VFH83_07000"
     CDS             complement(4572..7577)
                     /gene="pgmB"
                     /locus_tag="VFH83_07000"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HET6486149.1"
                     /translation="MDQASVYPVEPWKIREIGFNPSLALRNETVFALGNGNLGIRGSF
                     EEPFSPSAPGTYLNGFYDHTPILYGEVGYGWARNRQVMLNVADARCISLWIDGEPFDL
                     ATGTLLDYERCLDLSEGTVVRSLRWRSPFGSELILRSLRLVSLVRPSVAAIEWAVTPV
                     GKQASLVIESSIEGEVENRSSSDDPRVGAGFPKPPLTTVSRAASGWESILVQETRATR
                     MALACAVSHRLEAGGRGAQVTASAFAQRAVLRLEARCAPGTPLRLVKYIAYRTTLDAS
                     RDSIEEQARRQANLARTAGLSALQAEQKEEVARFWSAADVRIEGDDALQQGLRFNLFC
                     LFQYAGRNGKTSIAAKGLSGQGYEGHYFWDTEIYVFPFFLFTQPTIARSLLRYRVGIL
                     GSARARAAEMSQRGALFPWRTIGGEEASAYFPAGTAQYHIDADIAYAARQYVLATGDR
                     GFLLDGGAELLFETARLWADLGTYDPQRDGTFRINEVTGPNEYSVLVDNNLYTNLMAR
                     ENLSFAAEVARTLRTDRPDEYTRIAGALRLQDAEIEAWERAARGMRIPVDPARGIHAQ
                     DDSFLGRAPWDFERTPPDRYPLLLHYHYLVIYRHQVLKQPDVVLAQVLLGNRFTLAEK
                     KRNFDYYDPLTTGDSSLSPCIQSVAAAELGYAEKAYEYFSRTARMDLDDVNGNVSDGV
                     HTAAMAGTWFSVIRGFAGMRDYDARLSFAPRLPRSWNSLRFRLRFQSRLLEVTLTSRE
                     ATYHLLEGEPLSILHEGRELRLEPERAQSLSLVPVLECVVFDLDGVLTDTAELHYQAW
                     KRLSDEIGLPFDRTVNEELKGVGRQDSLRIILRHAGVSKDTGEVELLAEIKNGYYREL
                     LQTITPARILPGMAELLRELRRRGIKTALASVSRNAAQILERLDIASSIDFVLEAGTV
                     LKGKPDPEIFLRAAEGLGVPPRNCAGVEDARTGLQAIRAAGMFAVGIGADLPEAHWKL
                     PDTRGLTADELGRRFLEYQEAALGSARAS"
     gene            7670..>8140
                     /locus_tag="VFH83_07005"
     CDS             7670..>8140
                     /locus_tag="VFH83_07005"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6486150.1"
                     /translation="MVAPLAQQINTQGYTVLRGFLEPGVVESVRQAASGLVDALAAER
                     VGSGAVPRDFAEEPFETRLIRLFEGDMDQAPKQFRENLHLPGVFPLFFHPRLLDVVQG
                     ILGPEIRLYPNYTVRPKLPEWKGTEVLWHQDAAYTESWTPGAPVTSMHMVNVWTP"
BASE COUNT         1343 a         2637 c         2780 g         1380 t
ORIGIN      
        1 acgctcgtcc tccccgcgcc cgtgatagac ccgtatggcg ttggccaccc cccactgcgt
       61 gaaaaggtgc ggccactcga atcgatcgat gccgcagtcc ctggcgaggc ggacgtaccg
      121 ctggaccagg gaccagtcga aggcgtagcg ctccccggct gcctggaccg ccacgagctg
      181 cgtcggtcgc ttcacgccgt ccagaggtgg ggtcagggcc ggcacgtaga tggtatcctg
      241 accgtgctcc gcgacgttca cgagatactg gcgcagcagg gtccagaacc gctcctcgaa
      301 ccccgagagt cggtaccagt ccaggatggc gtccgcgtag aaccaatgcg tgacggggaa
      361 gtccttccga ggcgcaatgc gcgcctcgtg cacgatcacc tccgccgtga gctccgctac
      421 gctctccccc tggcagagaa ccgagagcgg gatcgagaat cggcccggct ttgcgtcccg
      481 atccacgcgc acggtgatcc agaacgcggc cgtctggccg gccggcagga gcgtctggga
      541 ttcttcgaac agggggtccg gcacgtaccc gggaatgtgg tccatgctgt cgagctcccc
      601 gtcgggagtc tcggtgttgt gatacggtac gggcacgaag cccaggcgtc ggacccgggc
      661 ggcgattccc gggggagcgg cggccgagac ggagacccgc gcggggtcac cgcgccccag
      721 gctctcgatg gcgagctgga acgacagacg ctcgccgcga ccggcttcga gccacggttg
      781 gccgtcggcc tgatccgcag gccggtttcc cgtcgggtag atccgaagca gggagtcctg
      841 gagccagcac cgcactcgca tggttgttct ttcactcctc gtcgtcgacc acccgttcgg
      901 gtggagattc gagctgccgg ggatcccgga gaagggtcgt caggtatcgg aaggcgctcg
      961 cgaagtagtg cccgtcacag atccgctcgt cgccgaccac cttgatgccg acagacctcc
     1021 gttcgacgat cgcgccattg cggtccgtca cgtgtcgacg ctccttgatg ccgaaggcga
     1081 cgaacacgga ggtcgtcccg aactcgtaaa ggtggtggta gatggggcgg atcccgaggg
     1141 agccgaggtc cgtgacgaag atgctggtat ggaaggggct ggcgcgatgg atggcccgag
     1201 gcagcttccc gtagaagtcg agcgtccgaa agagccagac caggaatcgg accaggaacc
     1261 ccggggccat catgaacagc cgcgcggtgg cgtccgtctc gttctgggtc tccaggtcct
     1321 tgttctccgc cacggcggcg ttgatccggt cagccacgtc gaagatcgtg tccgtgggca
     1381 ggaaggcgag cttgatcgtg gtttccgggc tgtcctcctg gagcttcttt ttcagggcaa
     1441 gggagatgag gatctcgttg cgcgcaaaga tgcgctggcc ggccacgaac cggttgaggc
     1501 gaggcttctg ggagagggtc cgcacggagg cggcgatgag cacgtgcaga agccccagct
     1561 tcgcgctccc ctggtcgcgc atctgccgca gccagcgctc ggcgggcccg atctccacct
     1621 ggtcctcgaa gtagacctgg gcatcgaccc gggatcgcat gatatagggt gtcatctggt
     1681 agagcgggtt gagggagcgt acccgtctgc cgtcatagcg gtccccccag cgccttcgag
     1741 ttcgggcctc gggacggcgt tcgcgctctg cggtcatcag ttgcggcctc ttgggggcag
     1801 tataggcgcg aacgccgtcc cggcatagac cgcctcattc ctcttgacgc cgcgggccgt
     1861 cggggcgaga attcccttgc catggatttc aagcggttct tcacaccgga ggaggcggag
     1921 cgggcgttgc cgctggtgag gcagatcgtg gaggacatcc tcggcacggc gcggaggatt
     1981 cgcgaactgc cggaagagga ttccgcggcc cggcgcctgg aggcggagct gcaggagcac
     2041 ctgctggaga tccaggagtt gggctgcttc ttcaaggact ggaacttctc ggtcggtctc
     2101 gtcgacttcc ccgcgatcat cgacggggag acggtattcc tctgctggcg cagcgacgaa
     2161 cgctccctgg agtggtacca cggcatcgac gacgggtacc agggccggcg ccggcttccg
     2221 ggggtcgccg cggccaactg actctcgcca tcagggaggc accgggccat gcgcacgcgg
     2281 ttcacgttga acggttcgtg gcggatgctg atcggggagc ggcatctgcg cgcggtcgac
     2341 gtcccttcct cctaccgccc ggtcggcgta gcgaccctgg agcgggagct tccggcggtc
     2401 gctccggcca aggggcggcg ggtctacctc tgcttcgatg gcgtggccaa caccggagaa
     2461 gtcgccgtgg acgggaccca tctcgggccc atgcttccct tcgtaccgta caccttcgac
     2521 atcacccgtc atggggcgcc cgcccacctg gtccgggtca ccctcacgga cctgggagct
     2581 acgttcgggt actcgccggg atgggagagt tacggcggga tcatccggga cgcctttctg
     2641 gtcgtcgcgg acgaggctgc cttctccgac gtctacgtgc agacctgggt cgcttccgac
     2701 ctggagacgg cgcgatgcgc ggtaagggtg gagtaccgca acttcgcgag cacggcggtc
     2761 cgaggcaccc ttcgagcgga gttgaccctg gaaggggacc gggtggccgc ggagcccgag
     2821 ccgagggcgg acctgctcat gccaccggat ggccagaacg gcgtcaccac gctggagttc
     2881 ggggtcagcg ggattcgcct ctggtccccg gagcgtccca cgctctaccg gctcaccctg
     2941 agcctcgagg acgaggacgg ccggcggatc gacacggagg aggtctccgt cgggtttcgg
     3001 gacttccggg tggatggcag acggttcctg ctcaacggcc ggccgatctt cctgaagggg
     3061 gtgtgccggc acgacctctg gcaggaccgg ggctacacgc tgaccccggc gcagatggag
     3121 caggacatcc tggcaatcaa gaggctgggc gccaactaca tccggctggt ccactacccc
     3181 caccatcgcg gcatcatcga cctggcggag aagcacggga tcctggtgac cgaggagccg
     3241 ggcttgtgga acatccaggt cttcgcaaaa gaccggactc gtctggagca gcacaagagg
     3301 gacagcctgg aggtcatgga acggaccatc cgacgggatc gaggatcgcc ggccgtgatc
     3361 gcctggctcc ttggcaacga gtgccatatg gaagacctcg ggctggagtt cctgaagcgc
     3421 gccgcggccc tctgccgatc cctcgattcg cggcgaccgg tctcgttctc ccacctctac
     3481 accaaggatc tggccgatgg caagaagccg tacgccgaag cggggctgga tttctacgat
     3541 taccatccgt acacgcccag cgtcgagaca ttcgctggag tcatgaaggt attcgacgac
     3601 aagcccctgg tcttcggcga gtggggaggt tggttcgttc gggacagtga gggactcctg
     3661 cgcatgttcg gcgatgcctt cgcccgggca tccgcgtttg gctcctcgga cgcgctccac
     3721 ctgaacggca ttgcctattg ggagtgggcg gacatgcggc agtacggaag gggctacccg
     3781 gggtgcgagg ccggcatcct taccgaagga ctggtggatg aggcgcgcaa ccccaagccg
     3841 gactaccagg tcatgaagga tatcttcacg gcgatcgacg ccggtggtcc gcttccttcc
     3901 cgagccccgc tgacgcctcg ggtcaccatg ccggcctccc tggacggtta tgctcccgcg
     3961 ggcaagcttg tctgtatcga cctgggtccg gtagtcgaag aggcggcgca gcggaaggcc
     4021 tgggaatcct tggaacccgg ccaccgcaat cctttcccgg tggttcccgc catcggacca
     4081 atcccgtttc gtccctccgg agcggacggc aggccggtcg tgctggggag tctcgctcca
     4141 gccgccgtga ttgccgtggg cgccccggtg cgggcctttc acgccctggg tcacgtcgca
     4201 gtccacgggc accccctcgt cggctccgtg ggtcagaggg tggcgcagta catcatccgg
     4261 tacgacgacg gagccgagga aacggtggac ctcctcaacg ctgttcacct tgcccggcag
     4321 aatgccgtct acgagggaac ccggatcgaa cccgtggcct tcgacgctcc ggtggtgctc
     4381 cgcgaggttc gggacccgga ttaccgccac ctggagcttc ggtattatcg cctggttccg
     4441 ggtcggcctg gcgccctgat caggagcctg cgctttgcca ccgacagcgg tgacgagttt
     4501 cggcccttgt tgtacgcgct gaccgtggag cgcggctgag gttcgggcgg cgcccagcgc
     4561 gccatccggt gctacgacgc gcgggcagag cccagcgccg cctcctggta ctccaggaaa
     4621 cggcggccca gctcgtccgc ggtcaacccg cgcgtgtccg ggagcttcca gtgcgcctcg
     4681 ggcagatccg cgccgatccc gaccgcgaac attccggctg cccggattgc ctggaggccc
     4741 gttcgcgcgt cctcgacgcc ggcgcagttt cgcggcggca ctccaagccc ctccgccgcc
     4801 cggaggaaga tctcaggatc cggcttcccc ttgagcacgg tccccgcctc caagacgaaa
     4861 tcgatggagg acgcgatatc gagcctctcg aggatctgcg cggcgttgcg gctcaccgaa
     4921 gccaacgcgg tcttgatgcc gcgccggcgc agctcccgca gcagctcggc catgcccggc
     4981 aggatccggg ccggggtaat ggtctggagc agctcgcggt aatacccgtt cttgatctcg
     5041 gccagaagct ccacctcccc ggtgtccttg ctgactccgg catgacggag gatgatccga
     5101 aggctgtcct gtctgcccac gcctttcagc tcctcgttca ccgtgcgatc aaagggcaga
     5161 ccgatctcgt cggacagccg cttccatgcc tggtagtgca gctctgcggt gtccgtgaga
     5221 acgccgtcca ggtcgaacac gacgcactcc aggaccggga ccaggctgag ggattgggcg
     5281 cgttccggtt cgaggcgcaa ctcccggcct tcgtggagaa tcgagagcgg ttctccctcc
     5341 agcaggtggt aggttgcctc gcgagatgtc agcgtgactt ccagaagtcg cgactggaac
     5401 cgaagacgga agcgaaggct gttccacgat cgaggcagcc gcggggcaaa ggagagcctc
     5461 gcgtcgtagt cccgcatgcc agcgaagccc cggatcacgg agaaccaggt gccggccatc
     5521 gcggcggtgt ggacgccgtc agagacgttt ccgttcacgt cgtccaggtc catccgggcg
     5581 gttcgggaga agtactcgta agccttctcg gcgtacccca gctccgcggc ggcgacactc
     5641 tggatgcacg gggaaagcga cgagtctccc gtggtgagcg gatcgtagta gtcgaagttg
     5701 cgcttcttct ccgccagggt gaaccgattg cccaggagca cttgcgccag gaccacatcg
     5761 ggctgcttga gcacctggtg gcgatagatg accaggtagt gatagtggag caggagaggg
     5821 tagcggtccg ggggagtccg ctcgaagtcc cacggtgccc gacccagaaa cgagtcgtcc
     5881 tgggcgtgga ttccccgcgc gggatcgacc gggatccgca tgccccgcgc cgcccgctcc
     5941 caggcctcga tctccgcgtc ctgcagccgt agggccccgg cgatgcgggt atactcgtcg
     6001 ggccgatcgg tccgaagggt tcgggcgacc tctgccgcga acgacaggtt ctcgcgggcc
     6061 atcaggttgg tgtagaggtt gttgtcgacc agcacgctgt actcgttggg gcctgtcacc
     6121 tcgttgatcc gaaaggttcc gtccctctgg ggatcgtagg tccccaggtc ggcccagaga
     6181 cgggccgtct cgaagagaag ctcagcccct ccgtcgagaa gaaacccgcg gtcccccgtt
     6241 gccagcacgt actggcgagc cgcataggcg atgtcggcgt cgatgtggta ctgggccgtg
     6301 cccgccggga aatacgccga ggcctcctct ccgccgatgg tccgccaggg gaagagcgcc
     6361 ccgcgctggc tcatttcggc agcccgggcg cgcgccgagc ccaggatccc cacccgatag
     6421 cgaagcaggc tccgagcgat cgttggctgc gtgaagagga agaaggggaa cacgtagatc
     6481 tccgtgtccc agaagtaatg gccctcatac ccctgaccgc tcaggccctt ggccgcgatg
     6541 ctggtctttc cgttacggcc cgcgtactgg aagagacaga acaggttgaa ccgaagcccc
     6601 tgctgcagcg cgtcgtcgcc ctcgattcgg acgtcggccg cgctccagaa ccgggcaacc
     6661 tcctccttct gctccgcctg aagcgcgctg agcccggcgg tccgcgccag gttggcctgt
     6721 cgtcgagcct gttcctcgat tgaatcccgg gaagcgtcca gggtggtgcg gtaagctatg
     6781 tacttgacca gccgaagcgg cgtacccggc gcgcagcgag cctcgagccg cagaacggcg
     6841 cgctgggcga aggcggacgc ggtgacctgc gccccgcggc cgcccgcctc cagacggtgc
     6901 gagacggcgc aggccagcgc catgcgcgtg gcgcgtgtct cttgcaccag gatggactcc
     6961 caccccgacg ccgcgcgcga gacggtggta aggggcggct tggggaaccc ggccccgacc
     7021 cgcggatcgt cagaggagga gcggttttcc acctcgccct cgatcgacga ttcgatcacc
     7081 agggaggcct gcttgcccac cggtgtgacc gcccactcga tggcagccac ggaaggacga
     7141 accagggaca ccaggcgcag gctgcgcagg atcagctcgc tcccgaacgg agaccgccag
     7201 cgaagggacc ggactacggt gccctcggac aggtcgaggc accgctcata gtcgagaagg
     7261 gtgccggttg ccaggtcgaa cggctccccg tcgatccaga gggatatgca gcgcgcgtcg
     7321 gcaacgttca gcatcacctg ccggttgcgc gcccacccgt atcccacctc accgtacagg
     7381 atgggggtat ggtcgtagaa gccgttgagg taagtccccg gagcgctcgg ggagaatggc
     7441 tcttcaaagc tcccccgtat cccaaggttg ccgtttccca gggcgaagac ggtctcgttg
     7501 cggagcgcca gggacgggtt gaacccgatc tcacggatct tccagggctc aacggggtag
     7561 accgacgctt gatccatagg gacctcggcg ggattgtaca cgaggagggt ggcccggtcg
     7621 attggacgtt gggaggtcac tgccgtacat tgccgacagg agaggtggcg tggtcgcccc
     7681 gttggcacaa cagatcaaca cgcaggggta tacggtgctg cgagggttcc tggagcccgg
     7741 cgtcgtggag tcggttcgtc aagcggcctc cggcctggtg gacgcgctgg cagcggagcg
     7801 ggtcggatcc ggggcggtcc cgcgcgactt cgcggaggag cctttcgaaa cgcgcctgat
     7861 ccgtctgttc gaaggagaca tggaccaggc gccgaagcag tttcgggaga acctccacct
     7921 tcccggcgtg tttccgctgt tcttccatcc tcggctcctg gatgtcgtcc aggggatcct
     7981 tggccccgag atccggctat accccaacta cacggttcgg cccaagttgc cggagtggaa
     8041 ggggaccgag gtgctctggc accaggatgc cgcttacacg gagagctgga cgccgggcgc
     8101 ccccgtgacc agcatgcata tggtgaacgt gtggaccccg
//
LOCUS       DASRZS010000282         5052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_927689, whole genome shotgun sequence.
ACCESSION   DASRZS010000282 DASRZS010000000
VERSION     DASRZS010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927689"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VFH83_07010"
     CDS             complement(<1..852)
                     /locus_tag="VFH83_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET6486151.1"
                     /translation="MTYKSVVASRRGGPEVLQVIENDLREPKPAEAQVRVLATSVGRT
                     DVNYRYGYSPLAPKPPFVPGYEIVGVVEAVGAGVTRVSVGDRVAALTGHGGYTEVIYL
                     GQEHLVRVPPSLAPEDVATLVLNYATAYQMLHRVAKAKAGDRMLVIGASGGVGTALLE
                     LGRLAGLEMFGVASGAKRGAVSALGAVAIDYHTEDFVQAIRRVHPDGVDFVLDGVGGD
                     SSGRSLAVLRRGGKLIGYAAPTGASSILVGLLKMALVNLLPNGKSAHFFGISALYARD
                     KRPFMEDL"
     gene            complement(849..1226)
                     /locus_tag="VFH83_07015"
     CDS             complement(849..1226)
                     /locus_tag="VFH83_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486152.1"
                     /translation="MDRSIPILIPAGLFVLTLAFGVWLGRAGKPYGGALLNVHKLAAL
                     AAVVVTAVLTYQVLRGSPGPTNAIALLVFAGVCFLALFATGALLSATSIDRTILSVVH
                     GILTVLALIAAGIAFYLLLGRNP"
     gene            complement(1246..1899)
                     /locus_tag="VFH83_07020"
     CDS             complement(1246..1899)
                     /locus_tag="VFH83_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486153.1"
                     /translation="MNRATRAIVSTLGPASALAGLDHGLFEALQGNTPTPGLFVQAIG
                     PGQRMWPNGTEDAFTLVPRFLVSGILAIVLSVAVAVWSVGFVHRKRGPAVLLLLYVLL
                     FLVGGGVAQVAFFIPVWLVSTRVNTPLSWWRKVLSERTRVAIGKAWRGSLIVACVLFL
                     LGLEIAIFGFVPGLTEPSAILAVNWSVVAASFVLLLFTFVAGFAHDIQQMGPRTRAS"
     gene            2003..3058
                     /locus_tag="VFH83_07025"
     CDS             2003..3058
                     /locus_tag="VFH83_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET6486154.1"
                     /translation="MSDRVSPPQSIEVSVTVLTQMFRYLADRKVDKDALLRSVGVDPA
                     SIASPDAYLPIETYLRIQDGAAEYTGDPCFGLHTGEFVEPGSWSILGYMMMNCKTLGE
                     AFEKSARYSRVIGNLIEGRAVIRLNRVKLVFFTPPQAPTMSRHCFEATFASTVRMMRT
                     LTGVKLSPLEVTFIYPKPASISEYERILCCPVRFAQRENSMTVAASIGRTPILAANPG
                     LLEYFERYAQEFLLEMDRKSESARRVTRIILSRLDDEALSIAKVAREMAVSVRTLQKR
                     LESEGVAFSDLLRATRERLAKKYLHENYSVEEITYLLGFSEPSVFRKAFKKWSGVTPG
                     EYRKADPAGLPRREYAS"
     gene            complement(3071..3715)
                     /locus_tag="VFH83_07030"
     CDS             complement(3071..3715)
                     /locus_tag="VFH83_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6486155.1"
                     /translation="MKEKAPYHHPQLRKALLKAALVELKQHGAADLSLRRIAALANVS
                     HAAPYRHFRNKQEILAALVWDTQEAFTTALRSARESASTPAARLFAIGEAYLKFARKN
                     PERLTLMFSEAGIAAMSSYPVEPTPEALLRYNSFGVLEDTVKECQEAGVLNAGSDSGP
                     LSILVWSVVHGLSAIEREGFLASLGSQRGLSVEQAHAMVMQEFRTLVERGTRNG"
     gene            3797..4348
                     /locus_tag="VFH83_07035"
     CDS             3797..4348
                     /locus_tag="VFH83_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET6486156.1"
                     /translation="MRILLLNGNPDPGNAAWERSLDAWARTQVREGNAVSRKDLRDLR
                     IRFCTGCWSCWYTTPGLCAIRDDMESLFPEILGSDLTVWASPLVLGNVSALVKKTQDR
                     FIPLLHPFFELDRWEMHHRRRYPKDIDMGLIVGKGPTDTDEDLQIVRTLHERLARNGR
                     GRLVLCQTIEYATEEMDHEKIGA"
     gene            4329..5012
                     /locus_tag="VFH83_07040"
     CDS             4329..5012
                     /locus_tag="VFH83_07040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET6486157.1"
                     /translation="MKRLALNGSPRGTKSNSRKIIGWMVEGMREAGAEEPQVLDLART
                     RELPEQRDAFLEADEVVLVFPLYTDSTPGIVKNFLDSLADAPADRLRGKRLALVVHSG
                     FPEAAHSQPVVRYLERVCTRLGMLHCGTAIRGGSEGLHLMPEQMTRKTHVLFSTLGRC
                     LAAEGRFDREAVSRLGRVVHLGWFQRLSMTLLKPTGLPDLYWIMMLKQHGAWKRRFDQ
                     PYASALARA"
BASE COUNT          970 a         1603 c         1625 g          854 t
ORIGIN      
        1 cagatcttcc atgaagggcc gcttatcccg ggcgtacaga gcgctgattc cgaagaagtg
       61 tgccgatttg ccgttgggaa gcaggttcac gagcgccatc ttcagcagcc caacgaggat
      121 cgatgaggct ccggtgggcg ccgcgtatcc gatcagcttg ccgccccggc gtagcacggc
      181 gaggctgcgg ccgctggaat cgccgcccac accatcgagc acgaaatcga cgccatcggg
      241 gtgcacgcgg cggatcgcct ggacgaaatc ctcggtgtgg tagtcgatcg caactgcgcc
      301 caaggcgctg acggcgccgc gcttggcgcc ggatgcgact ccgaacatct ccaggcctgc
      361 caggcggccc aactcgagca gcgcggtgcc gactccgccg ctggcaccga tcacgagcat
      421 ccggtcgccc gccttggcct ttgccactct gtgcagcatc tggtaggcgg tggcatagtt
      481 caagaccagc gtggccacgt cttccggtgc caggctgggt ggcacgcgca caaggtgctc
      541 ctgccccaga tagatcactt cggtgtaccc tccgtgaccg gtcagggccg ccacccggtc
      601 gccgacggat actcgggtca cgccggcgcc gacggcctcg acgaccccca cgatctcgta
      661 ccccgggacg aatggcggct tcggagcgag aggcgagtac ccgtagcggt agttcacatc
      721 cgtgcgcccg acgctggtgg ccagcaccct cacctgcgcc tcggccggct tcggctcgcg
      781 aagatcgttc tcgatgactt gcaggacctc gggtcctccg cggcggctcg caacgacgct
      841 cttgtaggtc aaggattcct ccccaggaga aggtagaagg cgatccccgc ggcaatgagc
      901 gccagaaccg ttaggattcc gtgtaccacg gacaggatcg tgcgatcgat gctcgtggcg
      961 ctcaacaggg caccggttgc gaagagggcc aggaagcaaa cgcccgcgaa gaccaggaga
     1021 gcgatggcgt tggtcggtcc gggtgacccg cgcaggacct ggtacgtgag caccgccgtc
     1081 acgaccaccg cggccagagc ggccagcttg tggacgttca ggagtgcgcc gccgtatggc
     1141 ttgccggctc gtcccagcca aacgccaaag gccagcgtca gaacaaaaag accggctgga
     1201 atgagaatcg gaatgcttct gtccatgtgg attcctcccg gaggctcagg acgctcgtgt
     1261 ccgtgggccc atctgctgga tgtcatgcgc aaaacccgcg acaaaagtga aaaggagaag
     1321 aacaaaggac gccgcaacga cggaccagtt gacggcgagg attgccgacg gctcagtcaa
     1381 tcccggtacg aatccgaaga tggcgatctc cagacccagg aggaaaagca cacaggcgac
     1441 aatcaaggaa ccccgccagg ctttaccgat cgcgaccctc gtccgttcgg atagaacctt
     1501 ccgccaccag gatagaggcg tattgacccg ggttgacacg agccacacgg ggatgaagaa
     1561 cgcaacctgc gcgacccctc ccccaaccag gaacaagagg acatacagca gaaggagaac
     1621 ggccgggccc cgcttgcgat ggacaaaccc gacggaccag accgcgacgg cgacgctcaa
     1681 gacgatggcc aggataccgc ttaccaggaa tcgcgggacc agcgtgaacg cgtcttcggt
     1741 gccgtttggc cacatccgct ggccgggccc gatcgcctgg acaaagagac caggggttgg
     1801 ggtgttcccc tgaagggcct cgaacaggcc atggtccagt ccggcgagcg cggaggcagg
     1861 gcccagcgtg gaaacgatgg ctcgggtggc gcgattcatt gcagctccat catcggcatc
     1921 ctaactccgg cgcaaggatc tccctatgac ctttcccgca gaaaagatgc tttttcacgc
     1981 aaagagcgct aagatggcct cgatgagcga tcgcgtttcc ccgccgcagt ccatcgaagt
     2041 ctcggtgacc gtcctgactc agatgttccg gtatctcgcc gaccggaagg tggataagga
     2101 cgcgctcctt cgctccgttg gggtcgaccc cgccagcatc gcctcaccgg acgcgtatct
     2161 ccccatcgag acgtaccttc gaatccagga cggggctgcc gagtacaccg gggacccgtg
     2221 cttcgggctg cacaccggag agttcgtgga gcctgggagc tggtccatcc tcggctacat
     2281 gatgatgaac tgcaagaccc tgggagaggc tttcgagaaa tcggcccggt actcgcgggt
     2341 tatcggcaat ctcatcgagg gccgagccgt catccgcctg aaccgggtca agctggtctt
     2401 cttcacaccg ccccaggcgc cgacgatgtc gcgtcactgc ttcgaagcaa cgttcgccag
     2461 caccgttcgc atgatgagga cgctgacggg ggtcaagctg agtcccctgg aagtgacgtt
     2521 catctacccg aaacccgcgt ccatctccga gtacgagagg atcctctgtt gcccggtccg
     2581 gtttgcccag cgtgagaact cgatgaccgt ggccgccagc atcgggagaa ctcccatcct
     2641 ggcggccaac ccgggcctgc tggagtactt cgagcggtac gcgcaggagt tcctgctcga
     2701 aatggaccga aagagcgaga gcgcgcggag ggtcacgcgg atcatcctct cccggctgga
     2761 tgacgaagcg ctatcgatcg ccaaggtcgc gcgggagatg gcggtgagcg tccggacgct
     2821 gcagaagcgt ctggagtcgg agggggtcgc gttcagtgac ctgctgcggg ccactcgaga
     2881 aaggctcgcc aagaagtacc tgcatgagaa ctactcggta gaagagatca cctacctcct
     2941 gggattctcg gagcccagtg tgttccgcaa ggccttcaag aaatggtccg gcgtcacgcc
     3001 cggggagtat cggaaggccg acccggctgg acttccgcgc cgcgaatacg cctcctgacg
     3061 cccaccgagg ctagccattc cgcgtccccc gctcgacgag cgttcggaac tcttgcatca
     3121 ccatggcgtg cgcctgctcc acgctgaggc ccctctgact gcccagggac gcgaggaaac
     3181 cctcacgctc gatcgccgac agcccgtgca ccacggacca gaccaggatg gaaagcgggc
     3241 ccgaatcgct tcccgcgttg agaaccccgg cctcctggca ttccttgacc gtgtcctcca
     3301 gaacgccgaa ggagttgtac cgcagtaagg cctccggtgt cggctccacc gggtaggagc
     3361 tcatggcggc gataccagcc tcagagaaca tcagggtgag ccgctccggg ttcttccggg
     3421 cgaacttcag gtatgcctcg ccgatggcga agagccgagc cgccggggtg ctcgcggact
     3481 cccgagcgct tctcagcgcc gtggtaaagg cctcctgggt atcccagacc agcgcggcca
     3541 ggatctcctg cttgttgcgg aagtggcgat agggggccgc gtggctcacg ttggccaagg
     3601 cagcgatgcg ccgcaaggac agatccgcgg ccccgtgctg cttcagctcc acgagggcgg
     3661 ccttcaggag cgccttgcga agctgcgggt gatggtaggg cgccttctcc ttcatgtcga
     3721 aagaaaaata gcagaagatg ttgacagcgt caacatctca tgatatgttt ccagtgtaaa
     3781 cataggagga ggtcgtatgc gcattcttct gctgaatggg aacccggatc ctggaaacgc
     3841 cgcgtgggaa cggtcgttgg acgcctgggc ccggacccag gtccgggagg ggaacgccgt
     3901 ctctcgcaaa gaccttcggg acctcaggat ccggttctgc accggctgct ggtcctgttg
     3961 gtacaccact ccgggactct gcgccattcg cgacgacatg gagagcctgt tccccgaaat
     4021 ccttggatcg gacctcacgg tgtgggccag cccgctggtg ttggggaacg tctcggcgct
     4081 cgtcaaaaag acgcaggacc gcttcatccc gctcctgcat ccgttcttcg agctggaccg
     4141 ctgggagatg caccaccgcc ggcgctatcc caaggacatc gacatggggc tgatcgtcgg
     4201 gaagggaccg acggacacgg atgaggatct gcagatcgtc agaacccttc acgaacggct
     4261 tgcgcggaac ggccgggggc ggctggtgct gtgccagact attgagtacg cgacggagga
     4321 aatggaccat gaaaagattg gcgcttaacg gatccccccg gggaacgaag tccaattcga
     4381 ggaagatcat cggctggatg gtcgaaggca tgcgtgaggc cggcgccgaa gagcctcagg
     4441 tcctggacct ggcgcgcacc cgggagcttc ccgaacagcg ggatgccttc ctggaggcgg
     4501 acgaggtggt cctggtgttt cccctctata ccgactctac gccagggatc gtgaagaact
     4561 tcctggattc ccttgccgat gcgcccgccg accggctgcg cggcaagcgt ctggctcttg
     4621 tcgtgcactc gggcttccct gaggcggcgc actcccagcc tgtggtccga tacctggaac
     4681 gagtctgcac gcgtctgggg atgctgcact gcgggacggc tatccgcggc ggatcggagg
     4741 ggctgcacct catgccggag cagatgaccc gcaagaccca cgtcctcttt tcgacgttgg
     4801 gtcggtgcct ggctgcggag ggccggttcg atcgggaagc agtgagccgt ctgggtcggg
     4861 tggtccacct gggatggttc cagaggctgt cgatgaccct tctgaagccg accggtctgc
     4921 cggacctcta ctggatcatg atgctgaagc agcacggggc ctggaagcgg cgcttcgacc
     4981 agccctacgc ctcggccctc gccagggcgt agagctcggt ggttcccatc ccggcgtgct
     5041 ggtcgccagc cg
//
LOCUS       DASRZS010000283         2647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_543806, whole genome shotgun sequence.
ACCESSION   DASRZS010000283 DASRZS010000000
VERSION     DASRZS010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543806"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFH83_07045"
     CDS             <1..226
                     /locus_tag="VFH83_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486158.1"
                     /translation="FGSAILATVFQRRTAGYAASDLQVVSNAFNVSFWWAIGFAVVAA
                     IPTLFLTKPGKPEPETTPKEAEQRQVASGT"
     gene            complement(294..587)
                     /locus_tag="VFH83_07050"
     CDS             complement(294..587)
                     /locus_tag="VFH83_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009216168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HET6486159.1"
                     /translation="MDRFLGIAVGCAALLIIGVFHPIVIKSEYHFGKNVWPAFLVLGA
                     GSLAASVLVTGALASSILGVLGFSLFWSIRELFEQARRVEEGRFPANPKRKRP"
     gene            complement(672..971)
                     /locus_tag="VFH83_07055"
     CDS             complement(672..971)
                     /locus_tag="VFH83_07055"
                     /inference="COORDINATES: protein motif:HMM:NF020581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HET6486160.1"
                     /translation="MLRRILELLAADRPMRMDELSRALGAREAEVESQLEQLTRLGFV
                     EDLASKAACGTESACAPESACARCGSRAGCAAASPSRIWALTEKGRRSALNGKPA"
     gene            complement(973..>2647)
                     /gene="feoB"
                     /locus_tag="VFH83_07060"
     CDS             complement(973..>2647)
                     /gene="feoB"
                     /locus_tag="VFH83_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HET6486161.1"
                     /translation="NLYLVAELLLLPSPVLLVLNMMDVAEKEGITVEPNVLESALGIP
                     VVPMAAARGKGIDALKEMLESMVLGRAEYTPNAPPILSAHKLVLEQLRALVANAVPAG
                     FPADWVALKLLEGDDELSEIMRQSLPADAWKTVAGLLYQHEDAVLDIAGARYQWIGRM
                     VRAAVVEPPTSRMGLTARLDRFLTHPLWGTLAFLLLLGGVFFLTFSIGGPLQSLLSGL
                     IHGLGDALRLWMKPMPAWIVELLASGVLGGVGMVLTFLPILAIFYAALGFLEDTGYMA
                     RGAYLTDRLMHLMGLHGKSFLPILLGFGCNVPAVLGTRIIESKRARLQTILLVPFVPC
                     TARMAVVAVLAPIFFGSSAFLVTWGLVGGNILILAVLGLLLHRFAFEDEHVAFVMELP
                     LYHLPNLKTIGIYVWQNLVGFLRKAGSIILVASLVVWGVSYFPTGVITTSYLGMVGKW
                     LEPVSRLMGIPWQVFIALLTSFAAKENTIATLSVLYGDIGRNLPMVVSTAAGLGLLVF
                     QMLFVPCAGTIASIRQESRSLGWTSFSVLLMLALSFGIGLAVYQVGRLM"
BASE COUNT          532 a          852 c          855 g          408 t
ORIGIN      
        1 ttttgggtcg gctattcttg caacggtctt tcagcgccgg acggcaggct acgccgcctc
       61 cgatcttcag gtggtctcca acgcgttcaa cgtttcgttc tggtgggcga ttggttttgc
      121 cgtagttgct gcaatcccca ccctgttcct gacaaagcca ggaaaaccag aaccggaaac
      181 gactccaaag gaagcagaac agcggcaggt ggcgagtggt acatgacgtc ctgcgctcgg
      241 atggtcagac agcccccacc gcggtaccct tgcgcgtact cagggggaac ccctcagggc
      301 cgcttgcgct tcggattggc agggaagcgg ccctcctcca cgcgccgagc ctgttcgaag
      361 agctcgcgga tggaccagaa cagcgagaag ccgaggactc cgaggatcga tgaggcaagg
      421 gcgccggtaa cgagcaccga cgccgcgagg ctgcctgctc ccaagaccag aaacgccggc
      481 caaacgtttt tcccaaaatg gtactcgctc ttgatgacga tgggatggaa gaccccaatg
      541 ataagcaggg ctgcacaccc caccgctatg ccgaggaatc ggtccatcgg ttatccttcc
      601 gccgttgttg gcaatgagtc gaccggatcg catgcggagc tggccatctc gccgtccgtc
      661 ggctgtcgac gctatgcggg tttcccgttg agcgcggatc ggcggccttt ttcggtcagg
      721 gcccagatcc ggctcggcga ggccgcggcg cacccggcac gggagccaca ccgagcgcat
      781 gcgctctcgg gggcgcacgc gctttcggtg ccgcacgcgg ccttgctggc gaggtcctcc
      841 acgaagccca accgagtcag ttgctccagt tggctctcga cctccgcctc gcgggcgccc
      901 agcgcccggg acagttcgtc catgcgcatc ggccggtcgg ctgccagcag ctccaggatc
      961 cggcgcagca tcctacatca gcctcccgac ctggtagacg gccaggccga tgccgaagga
     1021 cagcgcaagc atgaggagca cgctgaagct ggtccaaccc agagagcggc tctcctggcg
     1081 aatgctggcg atcgttcccg cgcaggggac gaagagcatc tggaagacca ggagcccgag
     1141 cccggctgcc gtggagacca ccatcggcag gttccgaccg atgtcaccgt agagcacgga
     1201 aagcgtcgcg atggtgttct ccttggcagc aaagcttgtg aggagcgcga tgaacacctg
     1261 ccatgggatg cccatcaggc ggctgaccgg ctccagccac tttccgacca tccccaggta
     1321 gctggtcgtg atcactccgg tcgggaagta ggagacgccc cagacgacca gggacgcgac
     1381 caggatgatc gacccggcct tgcgcaggaa tcctacgagg ttctgccaca cgtagatccc
     1441 gatggtcttg aggttcggca agtgataaag gggaagctcc atgacgaacg cgacgtgctc
     1501 gtcttcgaag gcgaagcgat ggagaaggag tcccaggacg gcgagaatca ggatgttgcc
     1561 acccacgagg ccccaggtaa ccaggaacgc ggaagagccg aagaagatcg gagccaacac
     1621 cgccaccacc gccatgcgag ccgtgcacgg aacgaacggc accagaagga tggtttgaag
     1681 ccgcgcccgc ttcgactcga tgatccgcgt gcccagcacc gcggggacgt tgcacccgaa
     1741 gccaagaagg atggggagaa agctcttgcc atggaggccc atcaggtgca tcaaccggtc
     1801 ggtgaggtag gcgccgcgcg ccatgtagcc ggtgtcctca aggaacccca gcgccgcgta
     1861 gaagatcgcg aggatcggaa ggaaggtcag gaccatgccc actccgccca ggacgccgga
     1921 cgcaagcagc tccacgatcc atgctggcat cggtttcatc cagaggcgga gggcatcgcc
     1981 cagaccgtgg atgagcccgc tcaacaggga ctggagcggc ccgccgatcg agaacgtcag
     2041 gaagaacacg ccccccagga gcaggagaaa ggcgagggta ccccagaggg gatgggtcag
     2101 gaagcgatcc agccgtgccg tcagtcccat gcgggacgtc gggggctcta cgaccgcggc
     2161 gcggaccatc cggccgatcc actggtatcg tgcgccagcg atgtccagga ccgcatcctc
     2221 gtgctggtag agcagcccgg cgacggtctt ccaggcatcg gcgggaaggc tctggcgcat
     2281 gatctcggac agctcgtcgt cgccctccag cagcttcagt gcaacccagt ccgcgggaaa
     2341 cccggccggg accgcgttgg ccaccagcgc cctcagttgc tccaggacga gcttgtgcgc
     2401 ggaaaggatg ggaggagcat tcggagtgta ctccgcccgc cccagcacca tgctctccag
     2461 catctccttc agggcatcga tcccctttcc ccgcgcggcc gccatcggca cgacggggat
     2521 tcccagggcg gattccagca cgttgggctc gacggtgatg ccctccttct ccgccacgtc
     2581 catcatgttc agcacgagaa ggactgggga cgggagcagg agcagctccg cgaccagata
     2641 gaggttg
//
LOCUS       DASRZS010000284         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_153911, whole genome shotgun sequence.
ACCESSION   DASRZS010000284 DASRZS010000000
VERSION     DASRZS010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153911"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1703)
                     /locus_tag="VFH83_07065"
     CDS             complement(<1..1703)
                     /locus_tag="VFH83_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486162.1"
                     /translation="MLRRAWLLLLLMAAPIAPLAAQVEASAVVRDWEALTRLAPRPEG
                     SANEKLTVAYVESRLADLGAGYTPFDFQESDFEHSFSTCLRVDIPGQSRDSVLVVAPL
                     NSPPDAAEGQDGSINLALALDLVARARQTPYPLGLTVLFLGAEYGDSDAYPMGTALFL
                     RDFQPGYRVAVLYLDLRAAPTAVVLNGGGRGIVSPYWLVNRCVDALRSADVPFALRAE
                     EYQLDRMGLPEGRSIIEPYLNAGFPTMGIQGEYGPADSAAPAERLAQLGTFLSRFLEA
                     GRAGIADEWDRHYLLFQIGSLSLIIPERVYLVCLLGGLGGMLLISLIFRRGLRKYMRT
                     LVRGAWAIVPIAGLSFVFLFAGTCVLQGILSLRGFPDLWTHAPLEFLGLKIAIALLLY
                     GALYPFMRRLPFPRRGSFYSAAAILFLLIDIVVVSAFNVSFTYYFLWALFFVFLSGMV
                     PNRYAKVLLFLPAPFWGLRALFVVFSLPSPSFGHFLLLSPVWGDLLVAGVCLPFILML
                     LRFGRLFPGRGLLRRGVREIGFAAVLFAGTYVLQGILSLRAFPDLWTHAPLEFLGLKI
                     A"
     gene            1791..2300
                     /locus_tag="VFH83_07070"
     CDS             1791..2300
                     /locus_tag="VFH83_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET6486163.1"
                     /translation="MEVLAEILQRRSVRSFKPDPVEKEPIERILEAGRLAPSAKNRQE
                     WRFVVIQKKEVRERIMSAAFNQEYVGQAPVILAICTTNIDYRMPNGHLSYPIDLALAA
                     AQILLQAVHEGLGTCCITTFDEQEVREILTVPFSMRVPLLILIGHPEAMPEPTPRKSL
                     KQITSRDHW"
     gene            2329..>2674
                     /locus_tag="VFH83_07075"
     CDS             2329..>2674
                     /locus_tag="VFH83_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486164.1"
                     /translation="MVYCVAELRNYKQLVRLRSASLPEVESAITSVLGGGAASAGPGV
                     WAVRLGEERECSPTAVAQLAWRLREFLSLRRKDLFGYSVLIASISEERRKEEILRGLL
                     ETGDRMDQLWISP"
BASE COUNT          556 a          858 c          902 g          358 t
ORIGIN      
        1 atggcgatct tgaggcccag gaactccagc ggcgcatggg tccacaggtc cgggaaggcc
       61 cgcagggaga ggatgccctg caggacgtag gtgccggcaa agaggaccgc ggcaaacccg
      121 atctcccgaa cgccgcgccg caagaggccc cgcccgggga acagccggcc gaacctcaac
      181 agcatcagga tgaacggcag acatacgccc gccaccagca gatcgcccca aaccggggac
      241 aggaggagga agtggccgaa gctgggggag ggcagggaga agaccacgaa cagcgcgcgc
      301 agaccccaga agggggcggg caggaacagc aggaccttgg cgtaccggtt ggggaccatt
      361 cccgagagga acacgaagaa cagggcccag aggaagtagt aggtgaagga gacgttgaag
      421 gcggagacca cgacgatgtc gatgagcagg aagaggatgg ccgcggccga gtagaagctt
      481 ccccggcgcg ggaacggcag tctccgcatg aacgggtaca gcgcgccgta caggaggagc
      541 gctatggcga tcttgaggcc caggaactcc agcggcgcat gggtccacag gtccgggaag
      601 ccacgaagag aaaggatgcc ttgcaggacg caagtgccgg caaacaggaa cacgaacgac
      661 aggccggcga tggggacgat ggcccacgct ccgcgcacca gggtgcgcat gtacttgcga
      721 agcccccgtc ggaagatcag cgagatgagg agcatgccgc ccagaccgcc gagcagacac
      781 accaggtaga cgcgctcggg gatgatcagg gagagcgacc cgatctggaa aagcaggtaa
      841 tgacggtccc actcgtcggc aatgccggcc cggccagcct ccagaaaccg gctcaggaac
      901 gtccccagct gcgccaggcg ctccgccggc gcggcggagt ccgccggtcc atattcaccc
      961 tggatcccca tggtggggaa acccgcgttc agatacggct cgatgatgga cctgccttcc
     1021 ggcagaccca tgcggtcgag ctggtactct tcggcgcgca gggcgaacgg cacatcggcg
     1081 gagcgcagcg catccacgca tcggttcacc agccagtaag gactgacgat cccgcgtcct
     1141 ccgccgttca gcaccaccgc cgtcggcgcc gcgcgaaggt ccaggtacag cacggccacc
     1201 cggtagccgg gctggaagtc cctcaggaaa agggcggtcc ccatcgggta ggcgtccgaa
     1261 tcgccgtact cggcgcccag gaagaggacc gtaaggccca acggataggg ggtctggcgc
     1321 gcccgagcca ccaggtccag ggcaagggcg aggttgatcg aaccgtcctg tccctccgcc
     1381 gcatccggtg ggctgttgag gggagccacc accaggaccg aatccctcga ctgcccgggg
     1441 atgtccaccc gcaggcatgt cgagaaagaa tgctcgaaat cggattcctg gaaatcgaac
     1501 ggcgtgtagc cggcgcccag atccgccagg cggctctcga cgtacgccac cgtcagcttc
     1561 tcgttggccg agccttccgg gcggggggcc aggcgggtga gcgcctccca atcccgcacc
     1621 accgcgctgg cttccacctg cgccgccagt ggggcgatgg gcgcggccat caggagcagg
     1681 aggagccagg cgcgacgcag cattcgggaa tcagcatagg ggaagggccc gcgctcgtca
     1741 actaaacctt gaaaaaccga cctgaatcga ctacgctagc gtagggaacc atggaagtac
     1801 tggcggagat ccttcagcga cgaagcgtgc gatccttcaa gccggacccc gtggagaagg
     1861 agccgataga acgcatcctg gaagcgggtc gtctcgctcc ctccgcaaag aaccgccagg
     1921 agtggcgctt cgtcgtaatc cagaagaagg aagtccggga gcggatcatg tcggcggcct
     1981 tcaaccagga gtacgtcggc caggccccgg tgatcctggc gatctgcacc accaacatcg
     2041 attaccggat gcccaacggg cacctttcct atcccatcga ccttgccctt gccgccgcgc
     2101 agatcctgct ccaggcggtc catgagggcc tggggacctg ctgcatcacc acgttcgacg
     2161 agcaggaggt tcgggagatc ctcacggttc cgttctccat gcgggtgccc ctgctcatcc
     2221 tcatcggcca cccggaagcg atgccggagc ccacgccccg aaagtccctg aaacagatca
     2281 cctcgagaga tcactggtag agggcgcccg gagctcccag acagcgatat ggtgtactgc
     2341 gttgccgagc tgcggaacta caaacaactc gtgcggctgc gctcggcaag cctgccggag
     2401 gtggagtcgg ccatcacctc cgtgctggga gggggcgccg cctcggccgg ccccggagtc
     2461 tgggccgtgc gcctggggga ggaacgggag tgctccccaa cggccgtggc ccagcttgcc
     2521 tggcgccttc gggagttcct gtccctgcgc cgcaaggacc tcttcgggta ttcggtgctg
     2581 attgcctcca tctcggagga gcgccggaaa gaggagatcc tccgcggcct cctggagacg
     2641 ggagatcgga tggatcagct ctggatctct ccgc
//
LOCUS       DASRZS010000285         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4392133, whole genome shotgun sequence.
ACCESSION   DASRZS010000285 DASRZS010000000
VERSION     DASRZS010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4392133"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /gene="fusA"
                     /locus_tag="VFH83_07080"
     CDS             complement(<1..1575)
                     /gene="fusA"
                     /locus_tag="VFH83_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET6486165.1"
                     /translation="MADLNRIRNIGIIAHIDAGKTTTTERILFYTGKTHRMGEVDDGE
                     ATMDWMDQEQERGITITAAATTCYWRDRQITIIDTPGHVDFTAEVERSLRVLDGAVAI
                     FCAVGGVEPQSETVWHQADRYGVPRIAYINKLDRIGADFTGVLEEMKAKLGTTPLPLM
                     LPIGSEAELEGVIDLVEMREIRWNQDDQGQTFQFSPIAAAREEIARHWRKNLLDHLTH
                     LSDELIELYLGGADIPPQLLRAVLRLGTLKRAFTPVLCGASLRNMGVQPLLDSVVDLL
                     PSPLDIPPTHGHHLKKNADVEVPCKPEAFALGLVFKLQTNREAGNLCYLRMYSGRFKE
                     GQTVYNVNKKKRERAGRLLRMHANHSQQLDQVEAGDIAVVIGLKGSQTGDTLGVEAFP
                     ILLEKMHFPEPVISVAIEPRTLSERAKLSQALATLALEDPTFSVKENEETGELIISGM
                     GELHLDVLVTRIFREFKVEARVGKPQVSYRETISAETTHTERYHRMIAGKEHSATITI
                     TVKPLARGEGNRFASDL"
     gene            1716..>2101
                     /locus_tag="VFH83_07085"
     CDS             1716..>2101
                     /locus_tag="VFH83_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET6486166.1"
                     /translation="MAAPAATAQAQVLAWVNQARQRTGLPPVESDDLLSTTAQAWSRY
                     LAATGLLSHTGSDGSRVLDRYRAVGGTDARVGEILGAGRNLGEIERAWLGSDSHRQLV
                     LEPFWTNVGWGSSKTSSGEEVWVVVF"
BASE COUNT          334 a          663 c          688 g          416 t
ORIGIN      
        1 aagatcgctc gcgaaccggt tgccctctcc gcgcgccaac ggcttcacgg tgatggtgat
       61 cgtcgcggaa tgctccttgc ccgcgatcat ccgatggtag cgctccgtgt gcgttgtctc
      121 cgcgctgatg gtctcccgat aggagacctg tggcttgccg acacgcgcct ccaccttgaa
      181 ctcccggaag atccgggtga ccagcacgtc caggtgcagc tctcccatgc cggagatgat
      241 gagctccccg gtctcttcgt tctccttcac ggagaaggtc gggtcctcca gggccagcgt
      301 ggccagtgcc tgggacagct tggctcgctc ggagagcgtc ctgggctcga tggccaccga
      361 gatcacgggc tcggggaaat gcatcttttc cagcaggatg gggaatgcct ccactccgag
      421 ggtatccccg gtctgggatc ccttcagacc gatcaccacg gcgatgtccc cggcctcgac
      481 ctggtcgagc tgctgggagt ggttggcgtg catccgaagg agccgcccgg cccgctcgcg
      541 cttcttcttg ttgacgttgt agaccgtctg gccctctttg aaccgaccgg agtacatccg
      601 aaggtagcag agattgccgg cctcccggtt ggtctggagc ttgaacacca gaccgagcgc
      661 gaacgcctca ggcttgcagg ggacctcgac gtccgcgttc ttcttcaggt gatgaccgtg
      721 ggtcggcggg atgtccagcg gagacggcag gaggtccacg acggagtcca gcagcggctg
      781 gacacccatg ttgcgaaggg acgccccgca gagcacgggc gtgaaggcgc gcttcagagt
      841 ccccagccga agcaccgcgc gaagaagctg gggagggatg tcggcgccac ccaggtagag
      901 ctcgatgagc tcgtcggaga ggtgggtaag gtggtccagc aggttcttcc gccagtgccg
      961 ggcgatctcc tcccgcgccg cggcgatcgg ggagaactgg aacgtctggc cctggtcgtc
     1021 ctggttccat cggatctcgc gcatctccac gaggtcgatc accccttcga gctccgcctc
     1081 ggagccaatg ggcagcatca acggcagcgg cgtggtgccg agcttggctt tcatctcctc
     1141 cagcacgccg gtgaagtcgg cgccgatccg atccagcttg ttgatgtagg cgatccgggg
     1201 caccccgtac cggtccgcct ggtgccacac cgtctccgac tggggctcca cgccgccgac
     1261 cgcgcagaaa atcgccacgg cgccgtccag cacgcggagg gatcgctcca cctccgccgt
     1321 gaaatccacg tgtcccgggg tgtcgatgat ggtgatctgc ctgtcgcgcc agtagcaggt
     1381 cgtcgcagcc gcggttatcg tgatgccgcg ttcctgctcc tggtccatcc agtccatcgt
     1441 ggcttcgccg tcatcgacct cgcccatccg gtgggtcttg ccggtgtaga acaggatacg
     1501 ctcggtggtc gtggttttcc ccgcgtcgat atgtgcgata atgccgatat tgcggatgcg
     1561 gttgaggtct gccattcgtt ttccggcgtg ctcccaagaa gaatcaggga tttgttcctt
     1621 gaactagtcc gtttctacta tataaaatcc gccgtatcgt ggatagagag cgtatccggc
     1681 tccgtctctg cggtttgctc atcctttgct gctgcgtggc tgcgccagcg gccaccgctc
     1741 aggcacaggt ccttgcctgg gtcaaccagg ctcgccagag gaccggtttg ccgccggtcg
     1801 aatccgacga cttgctttcc accacggctc aggcgtggtc ccggtatctc gccgccacgg
     1861 gtttgctcag ccacaccgga agcgatggct cgcgagtcct ggatcgctac cgggcagtcg
     1921 ggggcaccga tgccagggta ggggagatcc tgggcgccgg aaggaacctg ggcgagatcg
     1981 agcgggcctg gttggggagc gactcccatc gccagctggt cctcgagccc ttctggacga
     2041 acgtcgggtg ggggagcagc aagacctcga gcggagagga agtctgggta gtggtgttct
     2101 g
//
LOCUS       DASRZS010000286         3453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3237926, whole genome shotgun sequence.
ACCESSION   DASRZS010000286 DASRZS010000000
VERSION     DASRZS010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3237926"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VFH83_07090"
     CDS             <1..841
                     /locus_tag="VFH83_07090"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HET6486167.1"
                     /translation="ALLERAGAIANRCVLEDRPVRIVEATREELSRYPLRRDPGEDRD
                     VQRLIEIEEFDWSACGGTHARRTGEVGPIQILGAEKVRGLWRIRFLAGYRTLRYLAAA
                     HRILSDVAREHSLHPLAIPEAARQWKAEAVALTKEVSRLRAERGAAEARRLFDLTPAE
                     PSGVRVVRLYSENLGSEELRSLANTLVSLGPAIVLAGGREEDSQFWVFARSPELPGTA
                     ATWDAAGALKSWLSGLSGRGGGNMRFAVGTCRSPMHLETSQVTEDLHRFGVPTGTGDQ
                     PVR"
     gene            988..1566
                     /locus_tag="VFH83_07095"
     CDS             988..1566
                     /locus_tag="VFH83_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486168.1"
                     /translation="MKKLLVVLCILSLAGTAMASNRPQITVGGPGNPIMNGPRDNTCQ
                     YGFIDTAIGSGWTWGLGQQLGIQCTGPMLITGVGCYSEFIVQAGNVDVNVNGNNIAVV
                     QPVAGNNQWTINPPYQVGAGPVCVMLCPEGNFWGVTGEDYNSPPYGNSYWSSSCACSN
                     AFTDNNLTIWVNYTNSVPTQSTSWGRIQAMYR"
     gene            1939..2727
                     /locus_tag="VFH83_07100"
     CDS             1939..2727
                     /locus_tag="VFH83_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486169.1"
                     /translation="MFHKKMLAVLLATGLAGTALAGPTSSSPGIVHPTHPSGTPTNNT
                     VALLETTTTAVGEALSSLGIGFDDYQGPPFPTDLSQYVTVFLAMDGGLLEASDIQSLA
                     NYVSAGGVLSFIGGSCWQDYVQAMNTYLVQNDLDNYCWATVASTPDVRIVDANNGLAQ
                     GLPPTYNFSNASASYYQFRATDPRIFVAADNGDGYHMLFAKRVGNGTFGYCINSPYLS
                     YWSGADLTWLTQVVSNLYLNIGIGPVPTRKVSWGSLKAAFQRAN"
     gene            complement(2825..3166)
                     /locus_tag="VFH83_07105"
     CDS             complement(2825..3166)
                     /locus_tag="VFH83_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET6486170.1"
                     /translation="MSDCRQHQDSDRSRPPSPGPDPSACVFRAVVSGIVQGVSYRAFA
                     QRQAQALDLAGYARNLADGRVEVVALGPRAALESLLERLRSGPALSRVDGLEVDWDHQ
                     GEAPRPFAVRR"
BASE COUNT          569 a         1171 c         1114 g          599 t
ORIGIN      
        1 ggcactgctc gagcgcgcgg gggccatcgc caaccgctgt gtcctggagg atcgtcccgt
       61 ccgcatcgtc gaggcgacgc gcgaagagct ttcacgctat ccgttgcgga gggatccggg
      121 agaggaccgg gacgtccagc gcctgatcga gatcgaggag ttcgactggt ccgcgtgtgg
      181 aggtacccat gcccgccgca ccggggaggt cggtccgatc cagatcctgg gcgcggagaa
      241 ggtgcgagga ctctggcgca tccgctttct ggccgggtac cggaccctgc gctatctggc
      301 cgcggcgcac cgcatcctca gtgacgtggc ccgggagcat tcgctgcacc ctctcgccat
      361 ccccgaggcc gcgcgccagt ggaaagccga ggcggtggcc ctgaccaagg aggtgagccg
      421 gctccgggcc gagcgcggtg cggcggaggc ccggcggctc ttcgacctca cgccggcgga
      481 gccctcggga gtccgcgtcg tgcgcctcta ttccgagaac ctgggatcgg aggaactccg
      541 ttcactggcg aatacgctgg tctcccttgg ccccgccatc gtcctcgcgg gtgggcgaga
      601 agaagactcg cagttctggg tcttcgcccg ctccccggag ctgccgggaa cggcagcgac
      661 ctgggatgcg gcgggtgcgc tcaagtcctg gctctccggg ctttctggac gtggcggtgg
      721 aaatatgaga tttgccgtag gcacgtgtcg cagtccgatg catcttgaga ccagtcaagt
      781 gacggaggat ctccaccggt tcggcgtacc tacgggcact ggagatcaac ccgtacgatg
      841 agaagattgc cgatcgttct ccattagatt tgacttgcta tccggtatca tttccggcat
      901 actattcccg ggctagtcca accgggtatt cccattggga ccccgttggg aatactcgta
      961 caggggttca ctgttaggga ggtttccatg aagaaactgc tagttgttct ttgcatcctg
     1021 agccttgccg gcactgccat ggcatcgaac agaccgcaga tcaccgtcgg ggggcccggc
     1081 aacccgatca tgaatggtcc gcgggacaac acgtgccagt acggcttcat cgacacggcc
     1141 atcggcagcg gctggacctg gggtctgggt cagcagttgg ggatccagtg caccggtccg
     1201 atgctgatca ccggcgtcgg ctgctactct gagttcatcg tccaggcggg caacgttgac
     1261 gtcaacgtca atggcaacaa catcgcggtt gtccagccgg tcgccggcaa caaccagtgg
     1321 accatcaacc cgccgtacca ggtgggcgcc ggtccggtct gcgtcatgct ctgcccggag
     1381 gggaacttct ggggagtcac gggcgaagac tacaacagcc cgccctacgg caacagctac
     1441 tggtccagca gctgcgcctg ctccaacgcc ttcaccgaca acaatctgac catctgggtc
     1501 aactacacca actccgttcc gacccagagc acgagctggg gtcggatcca ggcgatgtac
     1561 cgctagtcgt atctccttcg catcaccagg gtttttggat ccgctcccag ggctcctggg
     1621 ggcggatctt cttttcgcgc ctggccgggt gtggctgccc gccgatcccg cggacgctcc
     1681 cggcgatggt ccggacttcc cgccggctgc cgccgacgag ggtcgcggag cttcctcgcg
     1741 gcctagctcc cggcatttcc cctgcctccg tccccgggat ggtgctacga ttgatctcgc
     1801 ttggtgtatc ctccggccgc gcccgcgaac gtggaatcca ctccccgagg aactccgtct
     1861 cttcctgcgc tgccggtctg cgtacgcgag gggcctttga gcccaacgcg cccggccagg
     1921 cggaagggag gtgaacgcat gttccacaag aagatgctcg cggtcctgct ggccacgggc
     1981 ctggcgggga ccgctctcgc cggccccacg tccagctctc cgggcatcgt ccatcccacg
     2041 catccctcgg gcaccccgac caacaacacg gtcgcgttgc tcgagaccac gaccacggcc
     2101 gtcggcgagg ccctgagcag cctcggcatc ggatttgacg actaccaggg accgccgttt
     2161 cccaccgacc tctcgcagta cgtcacggtg tttctggcca tggatggcgg cctgctcgaa
     2221 gcctcggaca tccagagcct ggccaactac gtgagtgccg gtggggttct ctccttcatc
     2281 ggcgggagct gctggcagga ctacgtccag gccatgaaca cctacctggt gcagaacgac
     2341 ctcgacaact actgctgggc cacggtcgcc agtacgcccg acgtgcgcat cgtcgacgcg
     2401 aacaacgggc tcgcacaggg gctgccgccg acctacaact tctccaacgc cagcgcgtcc
     2461 tactaccagt tccgggccac cgatccccgg atcttcgtgg ccgcggacaa cggcgacggc
     2521 tatcacatgc tctttgccaa gagggtcggc aacgggacct tcggctactg catcaacagc
     2581 ccctatctct cctactggtc cggggccgac ctgacctggc tcacgcaggt ggtctcgaat
     2641 ctgtacctca acataggtat cggacccgtg ccgacgagga aggtctcctg gggcagcctc
     2701 aaggccgcct tccagcgagc caactgatcg aaagcgctcg aggattcgcg actccgacgg
     2761 tcatacccag gacggcggga tcgctcccaa ggagagcccc ggttcaccca ggaccggggt
     2821 cttctcatct ccggacggcg aagggccggg gcgcctcgcc ctggtggtcc cagtccacct
     2881 cgaggccgtc cacccgcgag agggccggtc cggagcgcag gcgttccagc agggactcga
     2941 gcgcggctcg cggtcccagg gccacgacct ccacccggcc gtcggcgaga ttgcgggcgt
     3001 agccggccag gtccagcgcc tgggcctggc gctgggcgaa ggcccggtag gacaccccct
     3061 ggacgatgcc ggagacgacg gcccggaaga cgcaggcgga cgggtccggt cctggggacg
     3121 gcggccggga tcggtcggaa tcctggtgct gacgacagtc agacatgagc gcatctcctt
     3181 tcccaagggg aagcttcccg cccatggtaa catcggaacg gtccgtgcag cgcgggcccg
     3241 ggcgcgtctt cccctcatcg ttcccgggcg cggattcgca ggaggcggcg gtgtcaatgg
     3301 aatggattcg aacctcgttt ccgcaggcgc ggaccagcgc gcccggcgga gcgtatgctc
     3361 gcatccgcca cccgctgcgc gcggaagcgg ccgaaggggc gctcttcgcg ggactgccgg
     3421 cggacaccct cttcttcgat ctggagacgc tcg
//
LOCUS       DASRZS010000287         7157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2082666, whole genome shotgun sequence.
ACCESSION   DASRZS010000287 DASRZS010000000
VERSION     DASRZS010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2082666"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VFH83_07110"
     CDS             <1..1036
                     /locus_tag="VFH83_07110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6486171.1"
                     /translation="TMAMGIAIFALWISSVRRTRTGVFAAVAVSFWIVAGATDALGAL
                     SSQPLVPLPLLEYGAFFFSLSVLSVTLRDYFALFDVAERRRLDLEQAKSEAEAANRAK
                     SAFLAKMSHELRTPLNHIIGFTELVTSRGAGPLNDVQGEYLGDALDSGRHLLALVNDI
                     LDLSKVEAGKVTLTLREVDLGTLLSESVTVVQDRAAHQGLRLEARLDGPPVRILADEL
                     RLKQVLYNLLSNAVKFTPAGGSITLEMRRIQTPEHGDSVEVSVRDTGIGIRSEDLSRI
                     FNPFEQVKAEPGRSTQGTGLGLSVSRELVRLHHGEIRAESPGLGRGSTFVVLLPLPPA
                     VADGGPGQPV"
     gene            complement(1024..2541)
                     /locus_tag="VFH83_07115"
     CDS             complement(1024..2541)
                     /locus_tag="VFH83_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6486172.1"
                     /translation="MDREALLARVLSAPADFTDASTGLCGAPFFHAVLTREMARVRRT
                     GDPMAVGVVRVDGAADPKPAGSRGESTGPALGTIIRSHIREIDLATDCGGGLYLVQFC
                     GSRSESARSAAERIRAAVQGSGQGRTTVSVGLACFPDDGVETDLVITRAVDACLHAAN
                     AGGNRVFHFEKPAHSPSTAKARVLVVDDDARNLKLMCALLENQGFELVTATSGEQALE
                     VVRDLDVDLLLLDVMMPGVDGFDVCRRIKGTEATRQIPVVLLTALADSESRLKGVEAG
                     ADDFISKPANREELLARTRSLIRVKRLNRDLVSLENALVSLANAVEAKDHYTLGHTQR
                     VSTLAVAVGARLGLGDVELKGLRLGGILHDVGKIGVPEEVLNKPGPLSESEWVLMKSH
                     AELGYKICLPLLESIGPALDVIRHHHEKLDGSSYPDGLKGSGVSRMARIMAVVDCYDA
                     MVTDRPYRNGMPKEKALGILAQEVAQGRLDGEVLGVLESLVTGSRIGPDAPSQTG"
     gene            complement(2564..3988)
                     /locus_tag="VFH83_07120"
     CDS             complement(2564..3988)
                     /locus_tag="VFH83_07120"
                     /inference="COORDINATES: protein motif:HMM:NF045261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6486173.1"
                     /translation="MRSRFLKFAGVNGTEASPFPRELSYRFQFARLMLLAVAPLFYAY
                     AVHFYVIRLYLDSGFMLAGGLITSVSTFLGVRIGQQRRAVRFYYGVALSIILILLLHD
                     ADVVWRNGRLEYLVWLFLYPPLTFFLLGEKIGVYAVLVVSVVMAFVLLFPIPGVSVQV
                     NLTNLKIQFGVAFLSAIAGAYLYERTRRIALEELGTRQERLLRSERRLRETVDELRAS
                     NQKSEQLAVEARQASQAKSDFLAAMSHELRTPLHHIIGFTELVLEGVDGPLTDGQREC
                     LGDVVFSSRHLLSLIEDILDLSKVEAGKPQIHPSPVDLRALVERSVVMVRDRAQRHRI
                     TIGIELPAVPAVLVADQRMMRQIAYNLLSNAVKFTPDDGRVSLTLAVEANGGELEAAE
                     LRVTDTGVGIRPEDLERIFEPFVQVGATPGGGAEGTGLGLPLTRKLVELHGGRVWAES
                     EGLGKGSTFRVRLPAGRPSAGCQT"
     gene            4198..5550
                     /locus_tag="VFH83_07125"
     CDS             4198..5550
                     /locus_tag="VFH83_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013333.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET6486174.1"
                     /translation="MRSPAIQFGIHDIRLHVPSPRIGLESIVSRRVLEDPRLARHLER
                     ACRTTGQKAIRFPEPWEDSITMAAQASLSLMQGNPNLDPKSIRHLAVGTESGMDHSKA
                     ASAWLQGMLHRAGFALPASLSSFQVQHACAGGTMAMLAAGGLLLAGGRPEQTAIVAAS
                     DIARYETASTAEVTQGAGAAAMLLGSSPRLLSIDLATVGYFSADVDDFFRPIGAPTAR
                     VKGSYSMKCYWDSLEAAFLDHCRLAGEDPTERLAGTDFFALHTPFRNMPETALNRLLE
                     RRLGLDAEGIRTFLLSRGFYEGIDPIAEIGNLYTASLWTVVAFLLANRYQTLGEDIVG
                     KRMLLASYGSGNTMVVMSARVEAQAPRVIESWNLERVFESGRDASFEEYARWAEGSPS
                     VREYDALIDQAALPSGSFFLAGIREDGYREYRKAEELGTWIEQRDASRSLRRPLALQD
                     "
     gene            5507..6259
                     /locus_tag="VFH83_07130"
     CDS             5507..6259
                     /locus_tag="VFH83_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6486175.1"
                     /translation="MHPAVSEDPLRSRTEGGGEGWATAPRSRPARHVAWASLLFRSYL
                     RSLLKRSFHGLRLLGEAPEPPADLPIVLIGNHNTWWDGFLPYYVNTVILHRTFYILML
                     EEQLARYPFFRLLGAFGIRPGHPRSAMQTLRYCSSLLDRPDTLLCLFPQGVLVPFRAR
                     PLGFQRGLETILSIHGGPVTLLQFAMRCEFGGNRLPEAYLLFNPARVIDPRDFGGMEK
                     LERDQELLMDRLETEIARATPGRLLLGPIPKE"
     gene            6264..>7157
                     /locus_tag="VFH83_07135"
     CDS             6264..>7157
                     /locus_tag="VFH83_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6486176.1"
                     /translation="MKGIAASIIMQRLGGNRHRVNTVRDDLPLRLLQPKSVAVIGAGI
                     AGLTAATLLADRGFRVTVFEANAYLGGKAGSWIQELPDGFRARIDHGFHGFFRQYYNL
                     RTWMERMGSLGHLVPIEDYRISSPGHGVFSFKGISRTPLLNMLSLRTTGLYRLSDMMR
                     KPESRRLLAFLSYHPEGTFARYDEVSFESFSAAAGIPASMRMMFNTFSRSFFADPDLL
                     STAELIKSFHFYFLSNDLGLLYDYLDADFETGFTTPARRYLEQRGADILLSNPVDRVD
                     RVGAKLEIRGAPFDFAVLAADA"
BASE COUNT         1266 a         2408 c         2190 g         1293 t
ORIGIN      
        1 caccatggcc atgggcatcg cgatcttcgc gctctggatc tcctccgtcc gccgcacccg
       61 gaccggagtc ttcgccgctg tggccgtttc cttctggatc gtcgccggag ccacggacgc
      121 cctcggagcc ttgagcagcc aaccgctggt gccactcccc ctgctggaat acggcgcctt
      181 cttcttctcg ctctccgtgc tctcggtgac gcttcgagac tacttcgcgc tcttcgatgt
      241 cgccgaacgc aggcggctcg acctcgagca agcgaagagc gaagccgaag ccgccaaccg
      301 cgccaagagc gccttcctcg caaagatgag ccacgagctt cggacgcctc tcaaccacat
      361 catcggtttc acggagctgg tcacctctcg gggggccgga cccctgaacg acgtgcaggg
      421 agagtacctc ggggacgccc tggattccgg acgacacctc ctggctctcg tcaacgacat
      481 cctggatctt tcgaaagtcg aggcagggaa agtgacgctc accttgcggg aggtggatct
      541 ggggaccttg ctctccgaga gcgtcaccgt ggtgcaggac cgggccgccc accaggggct
      601 tcgtttggag gctcgcctcg acgggccgcc ggtccgcatc ctggcggacg agctccggct
      661 gaaacaggtg ctctacaacc tgctctccaa cgcggtgaag ttcacgcccg ccggcgggag
      721 tatcacgctc gagatgcgca ggatccagac ccccgagcac ggggacagcg tggaggtcag
      781 cgttcgcgat accggcatcg gcatccgctc ggaggacctt tcccgtatct tcaatccctt
      841 cgaacaggtc aaggcggaac cgggccgttc gacgcagggc accggtctgg gcttgtcggt
      901 cagtcgagag ctcgtgcgcc tgcaccacgg cgagatccgc gccgagagcc caggcctcgg
      961 aagaggctcc acctttgtcg tgcttctccc actgcctccg gcagtcgcgg acgggggacc
     1021 cggtcagccc gtctgagacg gcgcatccgg cccgatgcgg gatcctgtca ccagactctc
     1081 gagcacccca aggacctcgc cgtccagtcg cccctgggca acctcctgtg ccaggatccc
     1141 cagggccttc tccttgggca ttccgttgcg atacgggcgg tcggtgacca tggcatcgta
     1201 gcagtcgacg acggccatga tgcgggccat ccgcgagacc cccgacccct tgaggccgtc
     1261 cgggtaggag gatccgtcca gtttttcgtg gtggtggcgg atcacgtcga gggccggacc
     1321 tatggattcc agcagcggca gacagatctt gtagccgagc tcggcgtggc tcttcatgag
     1381 cacccactcg gattcgctca agggtcctgg cttgttcagg acctcctcgg gcacgccgat
     1441 cttgccgacg tcgtggagga tccctccgag ccgcaggcct ttgagctcga cgtcccccaa
     1501 ccccaggcgg gccccgacgg caacggcgag cgtggaaacc cgttgggtgt ggcccaaggt
     1561 atagtgatcc tttgcctcca cggcgttggc cagggagacc agcgcgttct ccaggcttac
     1621 caggtcccgg ttcagccgct tcacccggat caacgaccgg gtgcgcgcca gcagctcctc
     1681 ccgattggcg ggcttggaga tgaaatcgtc cgcccccgcc tcgacgccct tcagacggga
     1741 ctccgagtca gccagggccg tcaggagcac caccggaatc tgtcgggtgg cttccgtgcc
     1801 cttgatcctg cggcacacgt caaagccatc cacaccgggc atcatcacat ccaggagcag
     1861 caggtcgacg tcgaggtccc gcaccacctc gagagcctgc tccccagagg tcgccgtaac
     1921 caattcgaag ccctggttct cgagcagggc gcacatcagc ttgaggttcc gagcatcatc
     1981 atccacaacc agcacacgtg ccttcgcggt ggacggcgaa tgggccggct tttcgaagtg
     2041 gaagacgcgg ttcccgccgg cgttcgcggc atgcaggcag gcatctaccg ccctcgtgat
     2101 cacgaggtcg gtctcaaccc cgtcgtccgg aaagcaggcc agacccacgc ttacggtcgt
     2161 gcggccctgg cccgatccct gcaccgcggc gcgaatgcgc tcagccgcgg agcgggcgct
     2221 ctccgagcgg gacccgcaga actgtaccag gtaaagtccc ccgccgcagt ccgtcgcgag
     2281 atcgatctcg cggatatgcg agcggatgat ggtgccgagc gctggaccgg tcgattctcc
     2341 ccgcgatccc gcaggtttgg gatcggcggc accgtccacc cggacgacgc caaccgccat
     2401 ggggtccccg gttcggcgaa ctcgcgccat ctctctcgtc aggacggcgt ggaaaaaggg
     2461 ggcgccgcag agtccggtcg aagcgtccgt gaagtcggcg ggagccgaga gaaccctggc
     2521 aagcagtgct tctctgtcca tatgcctcca ggagccccgc cggtcaggtc tggcagccag
     2581 cggaaggccg acccgccggc agccgaacgc ggaaggtgct gcccttgccg aggccctcgc
     2641 tctccgccca caccctgccc ccgtggagct ccaccagctt gcgcgtcagg ggaaggccca
     2701 gcccggttcc ctccgcgcca ccgcctggag tagcgccgac ctgcacgaag ggctcgaaga
     2761 tcctctccag gtcttccggt cgaatcccca ctccggtgtc cgtcacgcgg agctccgcag
     2821 cctcgagctc gcctccattc gcctcgacgg cgagcgtcaa cgagaccctc ccgtcgtccg
     2881 gagtgaactt cacggcattg gacaacaagt tgtaggcgat ctgcctcatc atccgttggt
     2941 ctgccacgag caccgccggc acggctggca gctcgatccc gatcgtgatc cggtggcgct
     3001 gcgcccggtc cctgaccatg acgacgcttc gctccacgag cgccctcaga tccacgggcg
     3061 acgggtggat ctgaggctta cccgcttcca ccttggagag atcgaggatg tcctcgatca
     3121 gggagagcaa gtgcctgctg ctgaacacca cgtctcccag gcattcccgc tggccatccg
     3181 tcagcggacc atcgacgcct tccagcacca gctccgtaaa tccgatgata tggtgcagcg
     3241 gggttcggag ctcgtggctc atggccgcca ggaagtcgct cttggcctgg ctcgcttgcc
     3301 gcgcctccac cgccagttgc tcggacttct ggttggacgc ccgaagctcg tcgacggtct
     3361 cccgcaggcg gcgctcagag cgcaggagtc gctcctgcct tgtccccaac tcctccaggg
     3421 caatgcgccg ggtcctttcg tacaggtacg ctcccgcgat cgcgctgaga aaggctaccc
     3481 caaactggat cttcaggttg gtcagattga cctgcacgct tacgcccggg atcgggaaga
     3541 ggagcacaaa cgccatcacg acggagacga ccaggacggc atacactccg atcttctcgc
     3601 ccaggaggaa gaacgtgagc ggcgggtaga gaaaaagcca caccaggtac tccagcctcc
     3661 cgtttcgcca gaccacgtca gcgtcgtgca gcaggagtat caggatgatg ctcaaggcga
     3721 ctccgtagta gaaccgtacc gccctccgct gctgaccgat cctgacgccc aggaacgtcg
     3781 acaccgaggt gatcagcccg cccgccagca tgaatcccga gtcgaggtag agccgaatca
     3841 cgtagaagtg gacggcgtag gcgtagaaaa gcggcgcaac ggccaggagc atcagccggg
     3901 cgaattggaa gcgataggac agttcccggg gaaacgggga tgcctccgta ccgttgacgc
     3961 ccgcgaactt gaggaatctt gagcgcacgc gttcgagtag gttcaccgct tcagtgccct
     4021 cgcctccaac cgatggattc agatggattt ccacctaaag agtagcccgg gcgggctcgt
     4081 tttccactgg atttggcatc cagtcgaccg gtatattcac gaatagagtt acctgtggcg
     4141 ttttatgttg aatgaatact catttcgtgg tattatcaat ccagaacagg agcaaagatg
     4201 agatcgccag cgattcaatt cggcatccac gatatcaggc tgcatgttcc ctcacctcgc
     4261 atcggtctgg aaagcatcgt cagtcgacgc gtcctcgagg atccgcgact cgccaggcac
     4321 ctcgagcgcg catgccgcac caccggacaa aaggcgatcc gcttcccaga gccctgggaa
     4381 gactcgatta ccatggccgc ccaggcctcc ctttccctga tgcagggcaa tccgaacctt
     4441 gacccaaaga gcatccgcca tctggcggtg ggcaccgaga gcgggatgga tcactccaaa
     4501 gctgcgtccg cctggcttca gggtatgctg catcgggcag ggtttgccct gccggcctcc
     4561 ctttccagct tccaggtcca gcacgcatgc gccggaggga ccatggcgat gctcgctgcc
     4621 gggggtctgc ttttggccgg cggccggcca gagcaaaccg cgatcgtcgc ggcctccgat
     4681 attgcccgtt acgagaccgc ctccaccgcg gaggtcactc aaggagcggg ggccgcggcg
     4741 atgctccttg gctcctcgcc aaggctcctg tcaatcgacc ttgccaccgt tggatacttc
     4801 tcagccgacg tggatgactt cttcaggccg atcggcgcgc ccaccgcgcg cgtcaaggga
     4861 agctactcca tgaagtgcta ctgggacagc ctggaggcag cctttctgga ccactgccgg
     4921 ctggcagggg aagaccccac cgaacgcctt gccggcacgg acttcttcgc ccttcacaca
     4981 ccgtttcgca acatgccgga gacggcgctg aacaggctcc tggagcgacg actagggctg
     5041 gatgcggagg ggatccggac gttcctgttg tcgaggggct tttacgaagg gatcgacccc
     5101 atcgccgaga tcggcaacct gtacaccgcc tccctctgga ccgtggtggc ctttctgctg
     5161 gccaaccgat accagaccct cggtgaggac atcgtgggaa agcggatgct gctggcctct
     5221 tacggctcgg ggaacacgat ggtcgtcatg agcgcgcggg tagaggctca agctccccgg
     5281 gtcatcgaga gttggaacct ggaacgggta ttcgaatccg ggcgtgatgc ttccttcgag
     5341 gagtacgcgc gttgggcgga gggctccccg agcgttcggg agtacgatgc gcttatcgat
     5401 caagcagcgc tgccatccgg ctccttcttt ctggcgggga tacgggagga cggctaccgt
     5461 gagtatcgca aggccgagga gctcggcacc tggatcgagc aacgagatgc atcccgcagt
     5521 ctccgaagac cccttgcgct ccaggactga aggcggggga gagggttggg cgacagcccc
     5581 tcgaagccgc ccggcgcgac acgtcgcctg ggccagcctt ctgtttcgga gttatctgcg
     5641 gagtcttctc aagaggagct tccacggcct gcgactcctc ggcgaagcgc cggagccgcc
     5701 ggcggatctc cccatcgtgc tgatcggaaa ccacaacacc tggtgggacg ggtttctccc
     5761 ctactatgtc aacaccgtga tcctccaccg gaccttctac atcctgatgc tcgaggagca
     5821 gctcgctcgc taccccttct tccgtctgct cggcgccttt gggatccgac cagggcatcc
     5881 tcgcagcgcc atgcagacgc tgcgctactg ctcctctctc ctggacaggc ccgatacgct
     5941 gctgtgtctg ttcccccagg gcgtcctggt gccgtttcgc gcgcggccgc tgggcttcca
     6001 gcgaggcctg gagacgatcc tgtcgattca tgggggcccc gtgactctgc tgcagttcgc
     6061 catgcggtgc gagttcggag gcaatcggct gccggaggcc tacctcctct tcaacccagc
     6121 ccgcgtcata gatccccgcg acttcggcgg catggagaag ctggagcgcg accaggagct
     6181 tctcatggat cgcctggaaa cggagatcgc gcgcgcgacg ccgggaagac tgcttctggg
     6241 ccccatcccc aaggagtgaa ggtatgaaag ggatcgcggc ctccatcatc atgcagagat
     6301 tgggcgggaa ccggcatcgg gtgaacacgg ttcgtgacga tcttccgctg aggctgcttc
     6361 agccgaaatc cgtggccgtg atcggcgcgg gaatcgccgg tctgacggcc gctaccttgc
     6421 ttgcggaccg cggattccgg gtcaccgtgt tcgaagcgaa cgcgtacctg ggaggcaagg
     6481 ccggctcgtg gatccaggag cttccggacg ggttccgagc gcggatcgac cacgggttcc
     6541 acgggttctt ccgccagtac tacaaccttc ggacatggat ggagagaatg ggctccctgg
     6601 ggcacctcgt gccgatcgaa gactaccgca tctccagtcc tggccacgga gtcttctcct
     6661 tcaagggcat cagcaggacc ccgctcctca acatgctgtc gctccgaacg acgggcctct
     6721 atcgcctgag cgacatgatg aggaaaccgg agtcgcgccg cctgctcgct ttcctcagct
     6781 accacccgga gggcaccttc gctcggtatg acgaggtctc gttcgagagc ttctccgccg
     6841 cagccggtat ccccgcgtcg atgcggatga tgttcaacac gttcagccga tccttctttg
     6901 ccgacccgga ccttctgtcg accgccgagc tcatcaagag ctttcatttc tacttcctca
     6961 gcaacgatct ggggctgctc tatgactacc tcgatgcgga cttcgagacc gggttcacga
     7021 cacccgccag acggtacctc gaacagcgcg gcgcggacat cctgctttcc aatccggtcg
     7081 atcgcgtgga ccgggttggc gcgaagcttg aaatccgggg agcgcccttc gacttcgccg
     7141 ttctcgccgc cgatgca
//
LOCUS       DASRZS010000288         3303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3622188, whole genome shotgun sequence.
ACCESSION   DASRZS010000288 DASRZS010000000
VERSION     DASRZS010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3622188"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VFH83_07140"
     CDS             <1..995
                     /locus_tag="VFH83_07140"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET6486177.1"
                     /translation="FLQQVFRAVDAYTRKPVVLPVSKILMEGENVQLADLLLHTRDQG
                     RLPIDCSLAPLRDENGSIRGMVLAFRSVAERWKMQEIVGREVRQAIELQRSLLPPQGT
                     SLPGLQMRWFFFPAALAAGDLFNLFSIDPTHVGLYMVDAPGHGIASAVNSLLLHQFLS
                     IVPGQGTYLKLLDADALDPIQVAQKLASRLSTGGDLQSFSLVYGIIETESGSVTMVRT
                     GLPVPLVLRAKGPVEPATAWEPERREEFPPQFTGHRFQMGAGDRLFLYSDGLVECANP
                     DLEPFSQDRLAAQIEKVRGARLGDAVAALEDQARKWRGRTDFDDDICLMALQVG"
     gene            1105..1779
                     /locus_tag="VFH83_07145"
     CDS             1105..1779
                     /locus_tag="VFH83_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6486178.1"
                     /translation="MKKGIRLLIADDHTIVRKGIKVLVATEQDMQVIGEAENGAEAVE
                     KAGSLKPDVVLMDLVMPEMDGIEATRRITADHPGIKILVLTSFAADDKVFPAVKAGAL
                     GYLLKDSSPEQLLEAIRQVHRGEPSLEPSIARKVLQELSHPAKGAPTTDPLTEREMDV
                     LKLIAQGLSNKEIASRLFVAEWTVRTHVSNILGKLHLASRTQAALYALRSGLASLEDV
                     PAPEEP"
     gene            1790..>3303
                     /locus_tag="VFH83_07150"
     CDS             1790..>3303
                     /locus_tag="VFH83_07150"
                     /inference="COORDINATES: protein motif:HMM:NF044362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET6486179.1"
                     /translation="MNPIIPVDTLQAAQADHQAWRARALRTVLVVIAIGGVPALVSLI
                     LDAVLRDQATSLLAVYSVTYVALVALVFVPGLGLHARAWLFFVLAYILASASFARVGL
                     LGSGRLYLVLLPAVAIILLTPRAGFVCAGISLGVYALFAGLASAGALTPWVTEKQNPL
                     DLAFWVEAGAALVMFLLTLTLLLERLVRRHIQTLALSGRMTEELERAYRAMEMRVRDR
                     TRELGLLNSVAAVASGQVELGEVLRVSLERTMSAFGYEAGGAYGLDERTGTLVMLAHK
                     GLSEAFARQMEKLPLERALAGKELSLEQPLMWDVEEYPQGEMRRFIEAEGLTRVIGVP
                     LAARGRLVGGLVLNSRVLRQVSAEEGSLLIAVGQQIGLAMENARLLEAERLGREEVNR
                     RREVAEGLRETLAVLNSNKPLQEALDLIISQACRLMTCDASSLMQLDPVAGTLRIRAA
                     CGLDLDQVSAVELSLGPAGSRRALAWRQPVTVPDVTAMVEWQKQESNPDFAEDL"
BASE COUNT          487 a         1134 c         1125 g          557 t
ORIGIN      
        1 tctttctcca gcaggtcttc cgtgccgtgg acgcctacac gcggaagccc gtggtgctgc
       61 cggtcagcaa gatcctcatg gagggggaga acgtgcagct cgcggacctg ctcctgcaca
      121 cgcgggacca ggggcggctc cccatcgact gcagcctggc tccccttcgg gacgagaacg
      181 gaagcatccg cgggatggtg ctggcgttcc gcagcgtggc ggagcgctgg aagatgcagg
      241 agatcgtcgg ccgcgaggtg cgccaggcca tcgagctgca gaggagcctc ctccctcccc
      301 agggcacctc gctccccgga ctccagatgc gatggttctt cttccccgcc gcccttgccg
      361 cgggggacct cttcaacctg ttctcgatcg atccgacgca tgtgggtctc tacatggtgg
      421 acgccccggg gcacgggatc gcctccgcgg tcaactcgct tctgctccat cagttcctct
      481 ccatcgtgcc cgggcagggg acgtatctga agctgctgga cgcggacgcc ctggacccca
      541 tccaggtagc ccagaaactc gcctcccggc tctcgaccgg cggcgacctc cagtccttct
      601 ccctggtgta cgggatcatc gagaccgagt cgggatcggt gaccatggtc cgcaccgggc
      661 tgccggtgcc cctggtgctt cgcgccaagg gaccggtgga gcccgcgacc gcctgggagc
      721 cggagaggcg ggaggagttc cctccccagt tcacgggaca ccgcttccag atgggcgccg
      781 gcgaccgcct gttcctgtat tcggacggac tggtggagtg cgccaacccg gacctcgagc
      841 ccttctctca ggaccggctc gcggctcaga tcgagaaggt ccgcggcgcc cgtctcggag
      901 acgccgttgc cgccttggag gaccaggccc ggaagtggcg cggccgcacc gacttcgatg
      961 acgatatctg cctgatggcg ctccaggtcg gctgaagacc gagtcccagg gccatccgtt
     1021 cccgccaccg cccgcctctc gtgttctgcc gcttccccag ggcgtgagcg ggtgtgatat
     1081 actcggccca ccgagaggaa ctccatgaag aagggcatcc gtctgctgat cgcggacgat
     1141 cacacgatcg tgcgcaaggg catcaaggtg ctcgttgcca ccgagcagga catgcaggtc
     1201 attggggagg cggagaacgg ggcggaggcc gtggagaagg ccggttcgct caagccggac
     1261 gtcgtcctga tggacctcgt catgccggag atggacggga tcgaggccac gcgccggatc
     1321 accgccgatc accccggcat caagatcctg gtgcttacca gtttcgcggc cgatgacaag
     1381 gtgttccccg cggtaaaggc gggcgcgctg ggttacctgt tgaaggactc cagcccggag
     1441 cagctcctgg aggccatccg ccaggtgcat cgcggcgagc cgtccctcga gccttccatc
     1501 gcgcgcaagg tcctgcagga gctgtcccat cccgccaagg gcgcccccac gacggatccg
     1561 ctcaccgagc gggagatgga tgtgctcaag ctgatcgccc aggggctcag caacaaggag
     1621 atcgcctccc ggctgttcgt cgccgagtgg accgtgcgga cccacgtcag caacatcctg
     1681 ggcaagctgc acctggccag tcggacccag gcggctctct atgccctgag gtcgggcctg
     1741 gcctctctgg aagacgtgcc cgctcccgag gagccatagg ccccgcgcca tgaaccccat
     1801 cattccggtg gacacgctcc aggcggcgca ggcggatcac caggcgtgga gggcccgcgc
     1861 ccttcgcacg gtcctggtgg tgatcgccat cggcggcgtg cccgcgctcg tctccctgat
     1921 cctggatgcg gtcctgcggg atcaagcgac ttcccttctc gccgtgtatt cggtcacgta
     1981 cgtggccctc gtcgcgttgg tgttcgtgcc ggggctgggt ctgcacgccc gggcgtggct
     2041 cttcttcgtc ctcgcctaca tccttgcctc cgccagcttc gcgcgcgtcg ggctcctggg
     2101 cagcggccgc ctttacctcg tgcttctccc cgcggtggcc atcatcctcc tcaccccccg
     2161 ggccgggttc gtatgcgccg ggatcagcct cggggtatac gctcttttcg ccgggcttgc
     2221 cagcgcgggc gccttgacgc cctgggtcac cgagaagcag aacccgctcg acctcgcgtt
     2281 ctgggtggag gcgggtgccg ccctggtcat gttcctcctg acgctcaccc tgctcctgga
     2341 acgcctggtg cgcaggcaca tccagacgct cgccctcagc ggtcggatga cggaagagct
     2401 cgagcgggcc tatcgggcca tggagatgcg ggtccgggac cggacgcggg agctgggcct
     2461 cctcaactcc gtggccgcgg tcgccagcgg ccaggtggag ctgggagaag tgcttcgcgt
     2521 ctccctggaa cggaccatga gcgcgtttgg ctacgaggcc gggggcgcct acgggttgga
     2581 tgagcgcacc ggaaccctgg tgatgctggc tcacaagggc ctctccgagg cctttgcccg
     2641 gcagatggag aagcttcccc ttgagagggc cctcgccggc aaggagctga gcctggagca
     2701 gcccctgatg tgggacgtgg aggagtatcc ccagggagag atgcggcggt tcatcgaggc
     2761 cgagggtttg acgcgggtca tcggcgttcc cctggccgcc cgcggccggc tggtcggcgg
     2821 cctggtgctg aactcccggg tgctccgcca ggtcagcgcg gaggaaggat ccctcctgat
     2881 cgccgtgggc cagcagatcg ggctggccat ggagaacgcg cgcctcctgg aggcggagcg
     2941 gctgggccgt gaggaggtca accgccgacg ggaagtggcg gaggggctgc gggagacgct
     3001 ggccgtcctc aactccaaca agcccctgca ggaggcgctc gacctcatca tcagccaggc
     3061 ctgccggctc atgacctgtg acgcgtcctc tctcatgcag ctcgaccccg tggccggaac
     3121 gcttcgcatc cgcgccgcgt gcggcctgga cctggaccag gtctccgcgg tcgagctctc
     3181 cctgggcccg gcgggctccc gccgcgcgct ggcgtggcgg cagccggtca ccgtgccgga
     3241 cgtgacggcc atggtcgaat ggcagaagca ggagtccaat cccgatttcg cggaagacct
     3301 gag
//
LOCUS       DASRZS010000289         3881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2083568, whole genome shotgun sequence.
ACCESSION   DASRZS010000289 DASRZS010000000
VERSION     DASRZS010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2083568"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VFH83_07155"
     CDS             complement(<1..602)
                     /locus_tag="VFH83_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6486180.1"
                     /translation="MRLAGKVALITGAGRGIGEGIARAFVAEGAKVFVTDRNAEEGEA
                     LVREISRSSAGVWFLKLDVSVEQDWKDAMARVKERTGRLDVLVSNAGINIRDPIEQMT
                     EANLDAMLAVNVKGPFLGMKHAIPLLREAGGGSILCTSSICGLIGHRYTPEAYTMVKG
                     AVTLLARSVAVRYAKDNIRCNCVHPSTVETSLTGMMLSDPA"
     gene            complement(606..1550)
                     /locus_tag="VFH83_07160"
     CDS             complement(606..1550)
                     /locus_tag="VFH83_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET6486181.1"
                     /translation="MPYQANLREAWGQALLELARKKPAVVALEADLGKSTRSILMKAE
                     LPERYFEMGIAEQNMASTAAGLALAGKMPFIHSFAVFASGRAYDQLRNSICIPNLDVR
                     VCGSSAGLSDFGDGKTHQAVEDVAIMRALPNMTVLCPVDGIETALMVEALGRHHGPAY
                     VRINRNDLPFLYPPEEPYRIGKVLQLRDGDRAVVFACGVMVSRALQAAERLAASGIPT
                     RVVNVSTLKPLDREAIIRHAAGMQAIVTAEEHSVIGGLGSAVLEALRRERHAPLEMVG
                     IPDSFGVSASDYESLLGRFGLTAAAIEKAARGLLEGRA"
     gene            complement(1561..2406)
                     /locus_tag="VFH83_07165"
     CDS             complement(1561..2406)
                     /locus_tag="VFH83_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET6486182.1"
                     /translation="MQVRQTPAQLRQTAARLRLDVLRMIGYGKAGHLGGSFSLAEIVA
                     ALYFHVLRYDPKDIRNPDRDRFLLSKGHAVLIQYAALVELGVIPPEEMDRVKTVEGML
                     QGHPDMERTPGIEAVTGSLGQGLSIAVGMALALRLDRRPSRVYVIMGDGELSEGQLWE
                     AAMAATAHQVDNLTAIVDRNRIQATGPTIEVLPNPFIDDKFKSFGWSVTSMDGHDPSD
                     ILRALEAAGSVRGAPSLIIANTVKGKGVSFAENTAVFHNGILSKEQFEQARAELEAQL
                     RAAQG"
     gene            complement(2423..2995)
                     /locus_tag="VFH83_07170"
     CDS             complement(2423..2995)
                     /locus_tag="VFH83_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET6486183.1"
                     /translation="MADKNTLAGKIRSTREARSMTPEQLAERADLTVELVQNIESGAL
                     IPSLTPLIRIARALGARLATFLDDHEQTGPVISRVGERDAVMRVSGREQPSRSDLEFY
                     ALSANKASRRMEPFLVDIHPLSAQAPRLSSHEGEEFVYVLTGEIEISYGRDTYRLRTG
                     DCIYYDSIVPHHVHSVGTGDARVLAVIYAP"
     gene            complement(3097..>3881)
                     /locus_tag="VFH83_07175"
     CDS             complement(3097..>3881)
                     /locus_tag="VFH83_07175"
                     /inference="COORDINATES: protein motif:HMM:NF019469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1646 family protein"
                     /protein_id="HET6486184.1"
                     /translation="LRVVVGARVMAVTLVVAIGILSSIITAIIASLILVEIVRSMHLS
                     RKVETRLVIMACFSIGMGAALTPIGEPLATIAISKLAGEPYHAGFGFLLQRLWMYIVP
                     GIVVCGVIASFVMRTGTGQGDQDPPEEPERLREVFVRTGKVYLFVFGLVLLGTGFTPL
                     IDRIAGSVSHLVLFWANIVSAVLDNATLTAAEIVPAMALHQIVAALMGLLIAGGMLVP
                     GNIPNIIAAGRLKIDARAWAVFGLPVGLALMVACFAMLLLAR"
BASE COUNT          668 a         1312 c         1284 g          617 t
ORIGIN      
        1 gcgggatcac tcagcatcat gccggtcagg gaggtctcca ccgtgctggg gtggacgcag
       61 ttgcagcgga tgttgtcctt ggcgtagcgg accgcgacgc tccgggccag gagcgtgacc
      121 gcgcccttca ccatggtgta cgcctccggc gtgtagcgat gcccgatgag cccgcagatc
      181 gaagaggtgc agaggatgga tccgccgccc gcctcgcgca acaggggaat cgcatgcttc
      241 atccccagga agggtccctt cacgttcacg gccagcatgg cgtccaggtt ggcttcggtc
      301 atctgctcga tgggatcccg gatgttgatc ccggcattgc tcaccaggac gtccagccgg
      361 ccggtccgct ccttgacccg ggccatcgcg tccttccaat cctgttccac ggagacgtcc
      421 agcttgagaa accagacgcc ggcggaggat cgggagatct cccggaccag cgcctccccc
      481 tcctcggcgt tgcggtccgt gacgaagact tttgcgcctt ccgcgacgaa cgcgcgtgcg
      541 atgccttccc caatgcctcg acccgcgccc gtaatcagcg ccaccttccc ggccagccgc
      601 atgcctcacg ccctcccttc cagcagcccg cgcgccgcct tctcgattgc ggcggcggtg
      661 aggccgaatc gcccgagaag cgactcgtag tccgaggcgg agacgccgaa ggaatccggg
      721 atcccgacca tctcgagcgg agcgtgccgc tcccggcgaa gcgcctccag aacggcagac
      781 cccagaccgc cgatcaccga gtgctcctcc gccgtgacga tggcctgcat cccggcggcg
      841 tgtcggatga tggcttcccg atccaggggc ttgagcgtgc tcacgttcac cacgcgcgtg
      901 gggataccgg atgcggccag gcgttccgcc gcctgcaagg cccgggagac catcacaccg
      961 caggcgaaca ctacggcgcg gtcgccgtcg cggagctgga ggacctttcc gatccgatac
     1021 ggctcctcgg gcggatagag gaagggcagg tcgttgcgat tgatgcgcac gtacgcgggt
     1081 ccgtgatggc ggcccagcgc ctctaccatc agggccgtct cgatcccgtc caccggacag
     1141 aggacggtca tgttggggag ggcgcgcatg atagccacgt cctccaccgc ctggtgggtc
     1201 ttgccgtcac caaagtccga gaggccggcg ctcgagccgc agactcgcac gtccaggttc
     1261 gggatgcaga tggagttgcg aagctgatca taggcgcggc cggaggcgaa cacggcaaac
     1321 gagtggatga acggcatctt gccggcaagg gcaagacccg cggccgtgga cgccatgttc
     1381 tgctccgcga ttcccatctc gaagtagcgc tcgggaagct ccgccttcat gaggatggac
     1441 cgggtggact tgccgaggtc cgcctccagc gccaccaccg cgggcttctt tcgggccagc
     1501 tccaggagag cctgtcccca cgcctcccgc agattcgcct ggtagggcat gtcccctcct
     1561 ctacccctgg gcggcccgaa gctgggcctc cagctccgcg cgcgcctgct cgaactgctc
     1621 cttgctcagg atcccgttgt ggaagaccgc ggtattctcg gcgaaggaga ctcccttgcc
     1681 cttgacggta ttggcgatga tcagagacgg cgccccgcgc acggaacccg ccgcctccag
     1741 ggcgcggagg atgtccgacg gatcgtgccc atccatgctg gtcacgctcc agccgaagct
     1801 cttgaacttg tcgtcgataa agggattggg gaggacctcg atcgtcggcc cggtggcctg
     1861 gatgcggttt cggtccacga tggccgtgag gttgtccacc tggtgagcgg tggccgccat
     1921 ggcggcctcc cagagctggc cctcggaaag ctccccgtct cccatgatca cgtacacccg
     1981 actgggtcgt cgatccaggc ggagcgccag ggccatgccg acggcgatgg agagcccctg
     2041 gcccagggag ccggtcaccg cctcgatacc gggcgtccgt tccatgtcgg gatgcccttg
     2101 cagcatgccc tccaccgtct ttacgcgatc catctcctcc gggggaatga cgccgagctc
     2161 caccagcgcc gcgtactgga tcaggacggc gtggcccttg gagagcagga accggtcgcg
     2221 atcggggttg cggatatcct tggggtcgta gcgcaggacg tggaagtaga gcgcggccac
     2281 gatctccgcc agggagaacg agccgcccag gtgccctgcc ttgccgtagc cgatcatccg
     2341 aagaacgtcc agacggagcc gtgctgccgt ctgtcgcagc tgagcgggcg tttgccgcac
     2401 ctgcacctcg cacctcccct cgctacgggg cgtagatcac ggccaggacc cgcgcatccc
     2461 cggtccccac gctgtgcaca tggtgcggga cgatgctgtc gtagtagatg cagtccccgg
     2521 tccgaagccg gtacgtgtcg cggccgtagc ttatctcgat ctcgccggtc aggacgtaca
     2581 cgaactcctc tccctcatgg gaggagagac ggggtgcctg cgccgagaga ggatggatgt
     2641 cgaccaggaa aggctccatg cgtcggctcg ccttgttcgc ggaaagcgca tagaactcca
     2701 ggtcactgcg agagggctgt tcccgcccgg agacccgcat cacggcgtcc cgctccccca
     2761 cgcgggagat cacggggccg gtctgctcgt ggtcgtccag gaacgtagcc agccgcgcgc
     2821 cgagggcgcg ggcaatacgg atcagcggcg tcagggacgg aatgagcgcc ccggattcga
     2881 tgttctgcac cagctcgacg gtcaggtcgg cgcgctcggc gagctgctcc ggggtcatgc
     2941 tgcgcgcctc acgggtggac cggatctttc ctgccagcgt gttcttgtca gccacgatac
     3001 acctcgggcc aattcctcca cagaacccgc tttagatcaa gcgaccgggg cgcgtggccc
     3061 ggggcgcgta gcccggggcg cgcagcccgc cgacgcctat cgcgccagga gcagcatcgc
     3121 gaagcaggcg accatgaggg ccagccctac cggcagcccg aagaccgccc aggcgcgggc
     3181 gtcgatcttc agcctgcccg cggcgatgat gttcgggatg ttgcccggca ccaacatgcc
     3241 accggcaatg agcagtccca tgagcgcagc cacgatctgg tggagagcca tggccggcac
     3301 gatctccgcc gcggtgaggg ttgcgttgtc caggacggcg gacacgatgt tcgcccagaa
     3361 caacaccaga tgcgacaccg agcccgcgat gcggtcgatg aggggcgtga agcccgtacc
     3421 cagaagcacc aggccgaaca cgaagaggta gaccttgccc gtgcgtacga agacttcgcg
     3481 aagcctctcg ggctcctccg gcgggtcctg gtctccctgg ccggtgcccg tgcgcatgac
     3541 gaaggaggca atcacgccgc acacaacgat gcccgggacg atgtacatcc acagccgctg
     3601 gaggaggaag ccaaagccgg cgtgataggg ctcgccggcg agcttggaga tggcgatggt
     3661 ggccaacggc tccccgattg gggtgagggc ggcacccatc ccgatggaga agcaggccat
     3721 gatgaccagg cgcgtctcca cctttcggga gaggtgcatc gagcggacga tctccacgag
     3781 gatcagagaa gcgatgatgg ccgtgatgat gctggagagg atgccaatgg cgacgaccag
     3841 cgtcaccgcc atgacccgcg cgccgacaac cacacgcagg c
//
LOCUS       DASRZS010000290         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2472448, whole genome shotgun sequence.
ACCESSION   DASRZS010000290 DASRZS010000000
VERSION     DASRZS010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2472448"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFH83_07180"
     CDS             <1..522
                     /locus_tag="VFH83_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6486185.1"
                     /translation="ATDWIGRMQAALRDGAGVRALGDAPRGADPQDWVGRMAAARSGE
                     LRPGSVLEVERVQSALIQACSDRDLSAVLALFTPDATVRSGGRTYTGILQIRDYWAQT
                     AAFRSGSPWVGYAAPVSVRIETDPDRASVMFDWVWVNPETSRVAGLSRSEGVLVRVGR
                     TWMIEDLSSEGMG"
     gene            579..1718
                     /locus_tag="VFH83_07185"
     CDS             579..1718
                     /locus_tag="VFH83_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6486186.1"
                     /translation="MNRLGLVAAILTVSAVAAGHAAELKVAVLAPLSGPLAPFGMSTR
                     NGALLAVDQANARGGVLGLRVRAIVEDSQCAALPAEEAAKKVIHLDRVGFVIGEVCSK
                     ASLPVSDIANAARVIQISPASTNPAVTVDAHGSTKAYVFRACFIDPFQGTIGATFAFR
                     DLKARRAFILVDPNDAYGSGLTASFEAAFVRLGGRIAGKGTYGKDQTDFAAVLSTVRR
                     ARPDIVYLPDYFRIVNLVTRQARGMGIHAPFLGGDGWDSTELDARTVAEGYFTDHFSP
                     FDPRSEVRDFLVAYGSAFQTDAGLPRVPDGLAALAYDATGMLLEAIREAGSADTDRVR
                     SALERISYPGVSGTITFDRQHNPQKPAVILAVRAGRVDYYTTIQP"
     gene            1930..>2337
                     /locus_tag="VFH83_07190"
     CDS             1930..>2337
                     /locus_tag="VFH83_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6486187.1"
                     /translation="MNLIRYSPQPVDLFDNVSRWFDGFLSEPVFGRSSPAVDLRENDA
                     QYLMEVELPGLAEKDIEVKLESNLLTISSRKEEKTEEKKNGYLLRERRSAHFSRSFVL
                     PEDVEREKIAAEFKNGILTLTFPKVPAAQPKTIE"
BASE COUNT          415 a          712 c          782 g          428 t
ORIGIN      
        1 gccacggatt ggatcggcag gatgcaggcc gcgctgcggg acggggccgg cgtgcgggcg
       61 ctgggagacg cgccgcgggg cgcggatccg caagactggg tcggccgcat ggccgccgcc
      121 cgcagcggag agctgcggcc cggctcggtg ctggaggtgg agcgggttca gtcggctttg
      181 atccaggcgt gcagcgaccg ggacctgtcc gccgtgctcg cgctgttcac gccggatgcc
      241 accgtgaggt ccggtggacg aacgtatacc ggcatcctcc agatccgcga ctactgggct
      301 cagaccgcgg cattccgctc ggggagccct tgggtcgggt atgccgctcc ggtgtccgtc
      361 cgcatcgaga cggaccccga ccgggcctcc gtgatgttcg attgggtgtg ggtcaacccc
      421 gaaacgagtc gggtggcggg gctttcccga tccgagggag tgcttgtacg ggtcggccgt
      481 acgtggatga tcgaggatct gagctccgag ggtatgggat aggaccgggg aggggccggg
      541 ctcgctcccc catgaggtgt gaccggaaag gggacgggat gaatcgtctg gggcttgtag
      601 cggcaatcct tacggtttcc gcggtggcgg cggggcacgc cgccgagctg aaggtggcgg
      661 tccttgctcc cctttccggg cctcttgccc cattcggaat gtccacgaga aacggcgctc
      721 ttctcgcggt cgatcaggca aacgcgcgcg ggggcgtgct gggattgaga gtgcgtgcca
      781 tcgtggagga cagccagtgc gcggctctac ccgcggagga ggccgcgaag aaggtcatcc
      841 acctcgaccg tgttggcttt gtcatcgggg aggtctgttc gaaggcctcc ctgccggtct
      901 ccgacatcgc caacgcggcg agggtgatcc agatcagtcc ggcgtctaca aacccggccg
      961 tgacggtgga cgcccacggt tcgacgaagg cgtacgtgtt tcgagcctgc ttcatcgatc
     1021 ctttccaggg gacaatcggt gccaccttcg cgttccggga cctgaaggcg cgcagagcgt
     1081 tcatcctggt cgatcccaac gatgcctacg ggagcggcct tacggcatcc ttcgaggccg
     1141 cgtttgtacg cctgggtggg aggatcgccg gcaaagggac gtacgggaaa gaccagaccg
     1201 acttcgccgc cgtcctgtcc acggtgaggc gagcacggcc ggatatcgtg tacctgccgg
     1261 actacttccg gatcgtgaac ctggtgaccc ggcaggccag ggggatggga atccacgcgc
     1321 cgtttctggg gggagacggg tgggattcca ccgagttgga tgcccggacg gtcgccgaag
     1381 gctactttac cgaccatttc tctccgttcg accctcggtc cgaggtccgg gacttcctgg
     1441 tggcctacgg ctcggcattt cagaccgacg ctggcctgcc cagggttccg gatggactcg
     1501 cggcgctggc ctacgacgcc acaggaatgc tcctggaggc gatccgcgag gccggctccg
     1561 ccgacaccga ccgcgtgcgc tcagcgctcg agcgcataag ttaccccggc gtttccggaa
     1621 cgatcacgtt tgaccggcag cacaaccccc agaagcccgc cgtgattctc gccgtcaggg
     1681 ccggtagggt agactactac acgacgatcc agccctgaga gacagattgg gtcgccaaga
     1741 cacacaatgc caccaatgga cacaatgtga gtctttttga cacttaatgt agactgacct
     1801 gagaccaggg ccaacgcagt gggtatatgt aaatactttc tataattgta cttaagctaa
     1861 caggtctcct cttggcatgc ctgatgcatc tatagggccg tgaacttgaa aaccaatagg
     1921 aggtacgata tgaaccttat ccgttattcc ccccagccgg ttgacctgtt cgacaacgtc
     1981 tcccggtggt tcgatggctt cctctcggag ccggtcttcg ggcgctcctc tcccgcggtt
     2041 gatctgcgtg agaacgacgc ccagtacctg atggaggtcg agcttcccgg acttgccgag
     2101 aaggacatcg aggtgaagct ggagagcaac cttctcacca tctcctcccg caaggaggag
     2161 aagaccgagg agaagaagaa cggctacctg cttcgtgaga ggcgctcggc ccacttcagc
     2221 cgcagcttcg tgctgcccga ggacgtggag cgggagaaga tcgccgcgga gttcaagaac
     2281 ggcatcctga cgctgacctt cccgaaggtc ccggccgccc agcccaagac catcgaa
//
LOCUS       DASRZS010000291         5788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2086548, whole genome shotgun sequence.
ACCESSION   DASRZS010000291 DASRZS010000000
VERSION     DASRZS010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2086548"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VFH83_07195"
     CDS             complement(<1..1023)
                     /locus_tag="VFH83_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6486188.1"
                     /translation="MSTILIIDDEPGIRTVLRDVLQDEGHTVFLAEDGIRGLAELAAN
                     PTDLVFLDVWLPSLGGIDVLKRIREEHPDVEVIMISGHANVSLAVQATKMGAYDFLEK
                     PLSLDRTMTVVRNALAMEDLRRENRTLKNSLFMEDRMIGSSTGITAVRELIEQAARSD
                     SRILILGENGTGKELVAREVHARSGRAGGPFVELNCAAIPESLIESELFGHEKGAFTS
                     AVQRRRGKIEAAHRGTLFLDEIADMSLVTQAKVLRVLQEMRFERIGGEESISVDVRVI
                     SATNKDMGEQIRKGRFREDLYFRINVVPILVPPLRERLEDMPALVSYFMEKFKRPSAQ
                     EPKSVSE"
     gene            complement(1020..2789)
                     /locus_tag="VFH83_07200"
     CDS             complement(1020..2789)
                     /locus_tag="VFH83_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6486189.1"
                     /translation="MRGRPDRATIAENTSRGILILVVLFVLLIGVIIAFSRVFITAAT
                     GAEPAVASIALAVAVALPVVFLAITVYQLVKRLRRPMQGLRLRLTIFFILISVLSAGP
                     QALLAVTFINSAMGTWFRSSIGTALRGASTIAFDYQQEKQQALLSFLNAPHVTARMSQ
                     FAAAPEPAWRSIRELNTVIDSLQAFSEDGKEVAFRGDPRARLPDFASALVANGRDDRP
                     DVTILRAVRTLTVDARRLTVVVSVVLPASLRLNARQITESLTLFAALDRFRTLFQVVL
                     VGFFFLFSLPIFFITIQVSLLLTERILSPIVHLEEATLRVASGDFSTRILTRPRDELA
                     HLVDSFNSMVSELDRSRRKLLQAERITAWQEIAQRLAHEIRNPLTPIKLSAQRILKKH
                     EQNAVDLAEVLPPAVSSIIQEVENLERLLREFGEFAKLPVPKPSRVNLREMLTEVASV
                     YASLSSTVRISMDEVPPETVLHVDRNQMKRVFANLFTNAIQAMPKGGELAVRADLVRK
                     ARDSYFRIAVSDTGIGIPEENRDSIFDPYFTTKPDGTGLGLAIVQRIVFDHKGNVWAE
                     SNGGSGTTFFIDLPAPDAGGPAA"
     gene            complement(2786..3262)
                     /locus_tag="VFH83_07205"
     CDS             complement(2786..3262)
                     /locus_tag="VFH83_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486190.1"
                     /translation="MDLTVSRRSGALHAGVRFHGGPVDALAGSLRDGLESRITFTIRL
                     YERRAVPFLFGSDRLVMERTVVRSALWDFLDQSFVVESDTGTRSSYATVDALLEGFFT
                     LPDMVLPAPASSQGPRRYVVARAQFEPVALAPPLSLVTLVGAAATDTTPWARQEAP"
     gene            complement(3336..3602)
                     /locus_tag="VFH83_07210"
     CDS             complement(3336..3602)
                     /locus_tag="VFH83_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET6486191.1"
                     /translation="MVERTVTIRNRAGMHARPAALLVKTASGFSSQIFIEKDSERVNG
                     KSIMGVITLGATYNTPLKVIADGPDEVQALDAIERLFEAHFEEE"
     gene            complement(3609..4595)
                     /gene="hprK"
                     /locus_tag="VFH83_07215"
     CDS             complement(3609..4595)
                     /gene="hprK"
                     /locus_tag="VFH83_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HET6486192.1"
                     /translation="MSPQSKEKFRVLDLLQLDLRENDALDLKCIGGRPGLIREITVTE
                     INRPGLELGGFYENFAHQRLQIFGRGENAFLQKLAKEGRREELGKLFTYPIPCCVFTH
                     SLQPTRDFTEIAEKAECPTLQTGLSSSEFTMRILRVLSTIFAPKQAVHGTLVEVFGIG
                     VLLQGDSGVGKSEAALSLIERQHRLIADDLVEIRRISGNILMGSGAQVVSHHMEIRGL
                     GIINITHLFGVGAIRDRKQIQLVVQLETWNAEKNYDRIGAEENTVDILGVHVPCVIIP
                     IKPGRNVPVIIETAARNERLKKMGYYSAREFNRNVMNWLETESTRSQFLDGK"
     gene            complement(4604..4930)
                     /gene="raiA"
                     /locus_tag="VFH83_07220"
     CDS             complement(4604..4930)
                     /gene="raiA"
                     /locus_tag="VFH83_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET6486193.1"
                     /translation="MNVEIKGIHLEVTERIRDYIDAKMPRLEFAKDLIVDFLLNLTKE
                     KNLFKADVTVNFRWGASIHVGVQGFDLNRAIDDLFDRLDSKIEKEKSRIQDHHKSKEP
                     AREEKI"
     gene            complement(4927..>5788)
                     /gene="rpoN"
                     /locus_tag="VFH83_07225"
     CDS             complement(4927..>5788)
                     /gene="rpoN"
                     /locus_tag="VFH83_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HET6486194.1"
                     /translation="VKDVRESLLVQIRHHPTPHRLATVVVQDWFEALEKQKLKEIERG
                     LRINEATLKEVIAFLRSLDPLPGRTYSRDVPRYVIPDVQVRLQDGEFVIILNDEMVPV
                     LGLNPQFEEMAKNRAALKDAKTRQFVSRTIQDARWFIRTIGQRNETLLKVCRAIVEFQ
                     REFFLRGPKYLRPLTLKDIAAEVGVHEATVSRITSAKHMQTEWGVLSLKHFFSGSVAS
                     TGATGERFSKEGVKEIIREIIAQEGGEGLSDQKIVEALARRGVSIARRTVAKYRGELA
                     IDSSYQRRRT"
BASE COUNT          968 a         1885 c         1887 g         1048 t
ORIGIN      
        1 ctccgagacg gacttgggtt cctgggcgga cgggcgcttg aacttctcca tgaagtagga
       61 gaccagcgcc ggcatgtcct ccaggcgctc ccgcaaggga ggcacgagga tcggcaccac
      121 gttgatgcgg aagtacaggt cctcgcggaa gcggcccttg cggatctgct cccccatgtc
      181 cttgttggtc gcggagatca cgcgcacgtc cacgctgatc gactcttctc ccccgatccg
      241 ctcgaaacgc atctcctgca ggactcgaag gacctttgcc tgcgtgacca gggacatgtc
      301 ggcgatctca tcgagaaaga gggtaccacg gtgcgcggcc tcaatcttgc cccgtcgtct
      361 ctgaacggcg ctggtgaagg cgcccttctc gtggccgaag agctcgctct cgatcaggct
      421 ctccgggatg gcggcgcagt tgagctccac gaaagggccg ccggcccggc cgctccgagc
      481 gtggacctcc cgggccacca gctccttgcc ggtcccgttc tctcccagga tcaggatccg
      541 cgagtcgctg cgcgccgcct gctcgatcag ctcccgcacg gcggtgatgc cggtgctgga
      601 gcctatcatc cggtcctcca tgaagaggct gttcttcagc gtccggttct cccggcgcag
      661 gtcctccatg gccagggcgt ttcgcaccac ggtcatcgtt cggtccaggg agagcggctt
      721 ctccaggaag tcgtacgctc ccatcttggt cgcctggacg gcgagagaga cgttggcgtg
      781 tcccgagatc atgatgacct cgacgtcggg gtgctcctcc cggatccgct tgagcacgtc
      841 gatgcccccc aggctgggaa gccacacgtc caggaacacc aggtccgtgg ggttggccgc
      901 cagctcggcc agaccgcgga tcccgtcttc cgccagaaag accgtgtgac cctcgtcctg
      961 gagcacgtcc cgaagcaccg tgcggatgcc cggctcgtcg tcgatgatca ggatcgtgct
     1021 catgccgccg gtcctcccgc gtccggggcc ggcagatcga tgaagaaggt ggttcccgac
     1081 cctccattgc tctccgccca gacgttgccc ttgtggtcga agacgatgcg ctgcacgatg
     1141 gcgagcccca ggccggtgcc gtccggcttg gtcgtgaagt acggatcgaa gatgctgtcc
     1201 cggttctcct ccgggatccc gatgcccgtg tcgcttaccg cgatgcggaa gtagctgtcg
     1261 cgcgccttgc gcaccaggtc cgcgcgcacg gccagctccc cgcccttcgg catggcctgg
     1321 atggcgttgg tgaacaggtt ggcgaacacc cgcttcatct ggttgcggtc cacgtgaagc
     1381 acggtttccg ggggcacctc gtccatcgag atgcggaccg tcgaggagag actcgcgtag
     1441 accgacgcta cctccgtgag catctccctc aggttcacgc ggctgggctt ggggaccggt
     1501 agcttggcga actcgccgaa ctcgcgcagg aggcgctcga ggttctccac ctcctggatg
     1561 atgctggaca cggcgggggg aagcacctcc gccagatcca ccgcgttctg ctcatgcttc
     1621 ttgaggatcc gctgggccga cagcttgatc ggggtgagcg ggttgcggat ctcatgcgcc
     1681 agccgttggg cgatctcctg ccaggccgtg atgcgctccg cctggagcag cttgcggcgg
     1741 gaacgatcca gctccgagac catgctgttg aaggaatcca ccaggtgggc aagctcgtcc
     1801 cgaggacggg tcaggatgcg ggtgctgaaa tccccgctgg caaccctcaa cgtggcctcc
     1861 tccaggtgga cgatggggct gaggatccgc tccgtgagga ggaggctcac ctggatggtt
     1921 atgaagaaaa tcggaaggga gaacaggaag aagaacccga cgaggaccac ctggaagagc
     1981 gttcggaatc ggtccagggc ggcaaacagc gtcaggctct cggtgatctg acgggcgttg
     2041 agtcgcaggg aggccggcag gacgacgctg accaccacgg tgagccgacg cgcgtccacc
     2101 gtgagggtcc ttaccgcgcg aaggatggtc acgtccggcc ggtcatctcg gccgttggca
     2161 accagcgcgg aggcgaagtc cggcaggcgc gcccgggggt ccccccggaa cgctacctcc
     2221 ttcccgtcct cggagaacgc ctgcagcgag tcgatcaccg tattgagctc ccggatggac
     2281 ctccaggccg gctccggtgc agcggcaaac tgggacatcc gggcggtcac atggggtgcg
     2341 ttcaggaagc tcaacagggc ctgctgcttt tcctgctggt agtcgaacgc aatggtggag
     2401 gcgccgcgga gggccgtgcc gatagaggat cggaaccagg tgcccatggc cgagttgatg
     2461 aaggtcaccg ccagcagggc ctgggggccc gcggaaagaa cgctgatgag gatgaagaaa
     2521 atcgtgaggc gcagccgcag cccctgcatg ggcctccgca ggcgtttcac cagctggtag
     2581 acggtgatgg ccaggaacac aacgggtaga gccaccgcca ccgccagggc gatgctggcg
     2641 acggccggct cggccccggt ggctgccgtg atgaagaccc gggagaaggc aatgatgacg
     2701 ccgatgagga gcacgaacag caccaccagg atgaggatgc cccgggaggt gttctcggca
     2761 atcgtggcgc ggtccggccg ccctctcatg gcgcctcctg tcgagcccag ggcgtggtgt
     2821 ccgttgccgc cgcgcccacg agggtcacca gggagagcgg cggagcgagg gccaccggct
     2881 cgaactgggc gcgcgccacg acgtagcgcc gggggccctg ggaggaggcc ggcgccggaa
     2941 gcaccatgtc tggcagcgtg aagaacccct ccagcagcgc gtccacggtg gcgtacgagc
     3001 ttcgggtgcc cgtgtcggac tccaccacga agctctgatc caggaaatcc cagagcgcgc
     3061 ttcgcacgac ggtccgctcc atcaccaggc ggtccgatcc gaagaggaac ggaacggcgc
     3121 gccgctcgta cagccggatg gtaaacgtga tgcgggactc caggccgtcc cgaagcgaac
     3181 cggccagcgc atccaccggg cccccgtgga agcgcacccc cgcgtgcagg gcgccggagc
     3241 gccggctcac ggtgaggtcc acgctctggg cggcggcgag atacgggggc agcccgacca
     3301 ggaccagcgc ggcgaggaga ccggccgcgg ccggactact cctcctcgaa gtgcgcttcg
     3361 aacagccgtt cgatggcgtc aagcgcctgc acctcgtccg gtccatccgc gatgactttc
     3421 agcggggtat tgtacgtggc ccccagggtg atgaccccca tgatcgactt tccgttcacc
     3481 cgctcgctgt ccttctctat gaagatctgg gagctgaagc cgctggccgt cttgaccagg
     3541 agggcggcag ggcgggcgtg catgcccgcg cggttgcgaa tcgtgacggt gcgctccacc
     3601 atgtctgctc atttcccgtc caggaactgg ctccgcgtgc tctcggtctc cagccagttc
     3661 atgacgttgc ggttgaactc ccgggcggag tagtagccca tcttcttcag ccgctcgttt
     3721 cgagccgccg tctcgatgat cacgggcacg ttgcgtccgg gcttgatggg gatgatcacg
     3781 cagggcacgt gcacccccag gatgtccacc gtgttctcct ccgcgccgat gcggtcgtag
     3841 ttcttctccg cattccaggt ctccagttgg accacgagct ggatctgctt tcggtcccgg
     3901 atggcgccta ccccgaagag gtgggtgatg ttgatgatcc cgaggccccg gatctccatg
     3961 tggtggctca ccacctgggc gccgctcccc atgaggatgt tgccgctgat ccttcggatc
     4021 tccacgaggt catccgcgat cagtcggtgc tgtcgctcga tgagggagag ggcggcctcg
     4081 ctcttgccca ccccgctgtc cccctggagg agcacgccga tcccgaagac ttccaccagc
     4141 gtgccgtgga cggcctgctt gggcgcgaag atggtggaga gcacccgcag gatgcgcatg
     4201 gtgaactccg aggaggagag acccgtctgg agggtggggc actccgcctt ctcggcgatc
     4261 tccgtgaagt ctcgagtggg ctgcaggctg tgggtgaaca cacagcaggg gatggggtag
     4321 gtgaagagct tccccagctc ctcccgccgg ccctccttgg cgagcttctg gaggaacgcg
     4381 ttctccccgc ggccgaagat ctgcaagcgc tggtgggcga agttctcgta gaacccgccc
     4441 aactccagtc ccgggcggtt gatctccgtg accgtgatct cccggatgag acccggcctt
     4501 ccgccaatgc acttgaggtc cagcgcgtcg ttctcccgaa ggtcgagctg gagcagatcg
     4561 aggacgcgaa acttttcttt ggactgaggt gacatcgacc ctcctagatc ttctcctctc
     4621 gggcgggctc cttgctcttg tggtggtcct ggatgcggga cttctccttc tcaatcttgg
     4681 agtccagccg gtcgaacagg tcgtcgatgg cgcggttcag gtcgaacccc tgcacgccta
     4741 cgtggatgga ggcgccccat cggaaattga cggtgacgtc cgccttgaac aggtttttct
     4801 ctttggtgag attgaggagg aaatccacga tgagatcctt cgcgaactcc aatcggggca
     4861 tcttggcgtc gatgtagtcg cgaatccgct ccgtcacttc cagatgtatc cccttgatct
     4921 ccacgttcat gtccgtctcc tctgatagga cgagtctatg gcaagctcgc cgcgatactt
     4981 ggccaccgtc cggcgggcaa tcgatactcc ccggcgggcc agtgcttcca cgatcttctg
     5041 gtccgacaga ccctcccctc cctcctgggc aatgatctcg cgaatgatct ccttcacacc
     5101 ctccttggag aagcgctctc ccgtggcgcc ggtgcttgcc accgagccgg agaagaaatg
     5161 cttcagggaa agcacccccc actccgtctg catgtgcttg gcggaggtga tcctggagac
     5221 cgtggcctcg tgcaccccca cctctgccgc aatgtccttc aacgtcaggg gcctcagata
     5281 cttggggccc cggaggaaga actcccgctg gaactccacg atggcccgac agaccttgag
     5341 cagggtctcg ttgcgctggc cgatcgtgcg aatgaaccac cgcgcgtcct ggatggtccg
     5401 ggagacgaac tgccgcgtct tggcgtcctt cagcgccgcc cggttcttcg ccatctcctc
     5461 gaactgggga ttcaagccca gcaccggcac catctcatcg ttcaagataa tgacgaactc
     5521 cccatcctgg agcctcacct ggacgtccgg gatcacgtag cggggaacgt cgcgggagta
     5581 ggtgcggccg gggagggggt ccagggaccg caggaacgcg atcacctctt tcagggtggc
     5641 ctcgttgatc cgaaggccgc gctcgatctc cttgagcttc tgtttctcca gcgcctcgaa
     5701 ccagtcctgc accaccacgg tcgccaggcg gtgaggggtc ggatggtgac ggatctgcac
     5761 gaggaggctc tcccgcacgt ccttcacg
//
LOCUS       DASRZS010000292         3771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2858736, whole genome shotgun sequence.
ACCESSION   DASRZS010000292 DASRZS010000000
VERSION     DASRZS010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2858736"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            696..1424
                     /locus_tag="VFH83_07230"
     CDS             696..1424
                     /locus_tag="VFH83_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HET6486195.1"
                     /translation="MAKKIRVTVWNENRHEKSDQKIAAVYPKGIHGAIAEGLGADKDL
                     EIRTATLDEPSHGLTESVLASTDVLTWWGHMAHGDVSDAIAAKVQQRVLEGMGMVFLH
                     SAHMSKPFMRLMGTSCLLRWREAGEKERLWVVEPGHPIVDGLGPFFEIPHEEMYGERF
                     DIPNPESTVFISWFAGGNVFRSGCTFSRGKGRIFYFRPGHETFPTYYQKEVRQVIHNA
                     VRWVAPGDGPVFKLGDSEPPLEKL"
     gene            1432..2322
                     /locus_tag="VFH83_07235"
     CDS             1432..2322
                     /locus_tag="VFH83_07235"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6486196.1"
                     /translation="MAEPKIGWIGTGVMGRSMAGHLLKAGCPVSVFTRTPEKAAELLK
                     AGAVWCSTPAEAARGAEVVFTMVGFPSDVEQVYLGADGILSGARAGSLVCDMTTSEPS
                     LARRIHAEAKARSIASLDAPVSGGDVGAREARLAIMVGGEREAFERALPLFRRMGETI
                     ALMGGPGAGQHTKMVNQITIAGTMIGVVESLLYAQKAGLGLDAVIDIIGKGAAASWSL
                     NNLGRRIAKGDFNPGFYIKHFVKDMGIALQEARAMRLSLPGLALVNQLYISAQAQGLE
                     NLGTQGLYRVLAAMNGVQAP"
     gene            complement(2310..3698)
                     /locus_tag="VFH83_07240"
     CDS             complement(2310..3698)
                     /locus_tag="VFH83_07240"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HET6486197.1"
                     /translation="MLIRYDRLREALASPPARRARFRPAARLLEVFAGRFPGPSHRLV
                     AFGFVPGRIEVLGRHTDYAGGRTLVCAVDRGFLFAAAANRLGRVRLYEESSEFEPVDF
                     PLRPSITPEIGRWANYPMTMARRLARNFGSRLTGADIVLHGTLPVGSGMSGSSALMMT
                     TFTAIAALNRLSERETFRANLPSPVDLAVYLACAENGQGYHGLSGGAGVGTFGGSEDH
                     AAILMGKAGVLSLFAFRPASLLQEIPWPPHWRMIVAFSGVRAEKTREALEKYNLVSRR
                     ASLAVTRYNERTGRDLASLREIVADARAGTDRQWLERLDEGAEAGLFLADRVRQFMLE
                     DQRHLPAALRSIAARDIRGMGSALTQAHRASKRYLWNIAPEIDWLQRAAVRLGAAGAS
                     GFGAGFGGSILALADARGARDVARSWKGEYEKRHPERSRDASFFVAAPAPGIQLWTPD
                     GPSRLVDLVYGA"
BASE COUNT          694 a         1199 c         1280 g          598 t
ORIGIN      
        1 cgaagtacag cccggtggcg tccgctatgg cgccggcgtt accgctggag agggcgttga
       61 ctcgagagat cgtgacgaac acggtgaccg cgaagaccac gatcaccagg atcgaggtga
      121 gcaggaccag cttcgtgccg atcctcatcc tgttcaatgc gttcatggct tgtctcctcc
      181 aagagcccag cgaggttagt gggcgcttat cgaagcctgc actcaggatg cctgacttaa
      241 atgctaaggt acaagagacg tgccaagaga cggattatcg gctaattgtc aacgctgcaa
      301 ttgattggcg cgggggcgtc cgcacggttc ctgtttacat tggctcaacc tttctttaga
      361 tccagacgcc tccaagcgct atctcctcct cagctatata cttgggtgat ccttcaatga
      421 aagtttaggc ttggcgctgt tgagcgcgag acgcatcgcg tgaacgtgcg caaaggtacc
      481 acccgcagta ggggtgcagc tcggcggaaa ggcgggcctg cgcgaggcct gtggtgggcg
      541 gcgagtgacg acggtcaggt gccctgcggg ggggacgccg ggctgcgcta acacccttgc
      601 agcgcggcgc gattgaccct cccatcgatt tcctctatct agtaagcgca tcgcgcagaa
      661 ccatcacagg aagcccgcag cgacaggagg aatccatggc taagaagatc cgtgtcaccg
      721 tctggaacga gaatcgccac gagaagagcg atcagaagat agccgccgtc tatccgaagg
      781 ggatccacgg cgccatcgcc gaaggcctgg gagcggacaa agacctggag atccgcacgg
      841 ccaccctgga tgagccctca cacggcctga ccgaatcggt gctggccagc acggacgtgc
      901 tcacctggtg gggccacatg gctcacggcg acgtgagtga cgccatcgcc gccaaggtcc
      961 agcagcgcgt cctcgaagga atgggcatgg tgttcctcca ctcggcacac atgtcgaagc
     1021 ccttcatgcg gctcatgggg acgagctgcc tgcttcggtg gcgtgaggcc ggtgagaagg
     1081 agcgtctttg ggtggtggag ccggggcatc ccatcgtgga tgggctgggc cccttcttcg
     1141 agatccccca cgaagagatg tacggagagc gcttcgacat ccccaatccc gagtccacgg
     1201 tgttcataag ctggttcgcg ggaggaaacg tgttccgctc gggctgcacc ttcagccggg
     1261 gcaagggccg gatcttctac ttccgccccg gtcacgagac cttccccacg tactaccaga
     1321 aggaagtccg ccaggtcatt cacaacgcgg tgcgctgggt ggccccggga gacggtccgg
     1381 tgttcaagct tggagattcg gagccgcccc tggagaagct gtaggattcc catggctgaa
     1441 cccaagatcg gatggatcgg gaccggtgtg atgggccggt ccatggccgg gcacctgctg
     1501 aaagcgggct gcccggtcag cgttttcacc cggacccccg agaaagccgc tgaattgctg
     1561 aaggcgggcg ccgtctggtg ctccacgccg gcggaggcgg cgcgcggcgc ggaggtggtc
     1621 ttcacgatgg tcggcttccc atccgatgtg gagcaggtgt acctgggcgc ggacgggatc
     1681 ctgtcaggcg cgcgcgccgg ctccctggtg tgtgacatga ccacgtcgga gccgtcgctg
     1741 gcgcgtcgga tccatgcgga ggccaaggcg cgctccatcg cgtccctgga cgcgccggta
     1801 tccggcggag acgtgggggc gcgcgaggcc aggctcgcga tcatggtagg cggcgagcgg
     1861 gaggcattcg agcgcgcgct gccgctgttc cgacggatgg gagagaccat cgcccttatg
     1921 ggcgggccgg gcgccgggca gcacaccaag atggtgaacc agatcaccat cgcgggaacc
     1981 atgatcggcg tggtggagtc tctgctgtac gcgcagaagg ctggactggg gctggacgcg
     2041 gtcatcgaca tcatcggcaa aggcgccgcc gcgtcctggt ccctcaacaa cctgggacgc
     2101 aggatcgcca agggggactt caaccccggc ttctacatca agcacttcgt caaggacatg
     2161 ggcatcgcgc tgcaggaggc gcgggccatg cggctgtcgc tgccaggcct cgctctggtg
     2221 aaccagctct acatctcggc gcaggcgcag gggctggaga acctcggaac gcagggcctt
     2281 taccgggtgc tggccgccat gaacggcgtt caggcaccgt agacaaggtc tacgagccgg
     2341 ctcggtccgt cgggcgtcca gagttggata cctggcgccg gtgccgctac gaagaaggag
     2401 gcatctcgag agcgctcggg atgcctcttt tcgtactccc ccttccagct ccgcgccacg
     2461 tcccgggcgc ctcgcgcgtc ggcaagagcc aggatgcttc ccccgaagcc cgcgccaaac
     2521 cccgaagcgc ccgcggcgcc caggcggacg gcggcgcgct gcagccaatc gatctccggg
     2581 gcgatgttcc acaggtatcg cttcgaagcg cggtgggcct gcgtgagcgc ggagcccatc
     2641 ccgcggatgt cccgcgcggc gatggagcga agggcggcgg gcaggtggcg ctggtcttcc
     2701 agcatgaact gccgaacgcg gtcggcaagg aacagcccgg cctccgctcc ctcatccaac
     2761 ctctcgagcc attgacgatc ggtccccgcc ctggcatccg cgacgatctc ccgcagactg
     2821 gcgaggtcac gcccggttct ctcgttgtac cgggtcacgg cgagggaagc gcgccgtgaa
     2881 acgaggttgt acttctccag cgcctcccgg gtcttctccg cgcggacgcc ggagaaggcc
     2941 acgatcatcc gccaatgcgg gggccagggg atttcctgca acagggaggc gggtcggaac
     3001 gcgaacaggg agagcacccc agcctttccc atcaggatgg ccgcgtggtc ctcggagccc
     3061 ccaaacgtgc cgacccccgc tccgcccgac agaccgtgat acccctgccc gttttcggcg
     3121 cacgccaggt agaccgcgag gtccacggga gacggcaggt tcgcccggaa cgtctctcgc
     3181 tccgaaagcc ggttcagagc ggcgatggca gtgaaggtgg tcatcatgag cgccgaggat
     3241 ccactcatcc cgcttcccac cggaagggtg ccatgcagca caatgtccgc gccggtcaga
     3301 cgggacccga agttcctggc cagccgccgg gccatggtca tggggtagtt ggcccatcgg
     3361 ccgatctcag gcgtgatcga cggccgcagg ggaaaatcca cgggctcgaa ctcgctggac
     3421 tcctcgtaca ggcgcacacg gcccagacgg ttggccgcgg cggcgaagag gaagccacgg
     3481 tcgaccgcgc agaccagggt ccgaccgccg gcgtagtccg tgtgccggcc gagcacctcg
     3541 atcctccccg gcacgaagcc gaaggctacg agacgatggg aggggcccgg gaaccggccg
     3601 gcaaaaacct ccagcaggcg cgccgccggg cgaaagcggg cgcgtcgcgc tgggggactc
     3661 gccagagctt cccgaagccg gtcgtatcgg atgagcatgg gtagatggag catagaacga
     3721 cgagagaaac cgcgcaacgc cgtgcggacg gaaaccctca ccgggtgacc a
//
LOCUS       DASRZS010000293         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_162260, whole genome shotgun sequence.
ACCESSION   DASRZS010000293 DASRZS010000000
VERSION     DASRZS010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162260"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..>3233)
                     /locus_tag="VFH83_07245"
     CDS             complement(64..>3233)
                     /locus_tag="VFH83_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET6486198.1"
                     /translation="GDLQRCLIVCPGSLAEQWQDEMNQRFHLPFEILTNDRLEASRTG
                     NWFLEADQIIARLDKLARDDELQQKLAAPDCRWDLVVCDEAHKMSATFFGGEVSYTKR
                     FQLGRVLSTLTRHFLLMTATPHNGKEEDFQLFMSLLDGDRFEGKFRDGVHTSDASDLM
                     RRMVKEQLLKFDGRPLFPERIAKTVPFKLSEAEAELYAEVTSYVREEFSRAEALQNDK
                     RAGTVGFALTILQRRLASSPEAIYQSLLRRRERLESKLKELELIQRGFAVGEGLGGGR
                     SLDPDDLEDLEEAPDSEVEQAEEEVLDQATAARTIVELRAEIVTLRHLEALAGMVRRS
                     GNDTKWKQLAGLLSELLSGAPGSTDAADKEPRPKPSPRQKIVIFTEHRDTLNYLRLRI
                     TTLLGREDAVVTIHGGMGREERLSNQEAFKFQPEVQVLVATDAAGEGINLQRAHLMVN
                     YDLPWNPNRLEQRFGRIHRIGQTEVCFLWNLVATETREGDVYHRLLEKLDEARKALGG
                     QVFDVLGKLEFEGKSLRELLIQAVREGERPEVRERLNRVVENAVDRRKLQGLLEEHAL
                     VHDSMDVSRVRKIREDMERAEARRLQPHFIESFFIEAFSRLGGTMRQREPRRYEITHV
                     PAIVRSRDRLIGTREAVLSHYERATFEKDLIAPPGQPLATFVCPGSPLLDSVIDLTLE
                     RNRDLLKRGTVLVDEGDSSTEPRIFFYIESPIQDASTTRSGERRVVSKRVFHIEMDAR
                     GRTQGHPPRPLPRLPALAEGDPCIEAILARPECAWISRELEGSAQSYAIMHIVPEHVE
                     EVKTGRLERIAKTEAAVKDRLGKEINYWDHRAEELKAQEQAGKPNARLNSMGARKRAD
                     LLQGRLQKRIEELKLEAQIAPLPPVILGGFVVVPVGLVRAMKGEEPVVAADSALLPII
                     HVSAETPASAAADKQAAAARARAIVMEVERSLGFEPVDRETEKVGYDIESRVPGTGKL
                     RFIEVKGRVAGAQTLTVTHNEIRFSLNKPEDFILAIVEFVEGGEGGAHRMHYLREPFA
                     REPDFDVASVNYYYAQLLARAGKPS"
BASE COUNT          489 a         1134 c          985 g          625 t
ORIGIN      
        1 tcagaagcgc catggctggt ttcgttccgt acggtgccct gtccttgagg cggacccccg
       61 ccctcacgac ggcttccccg cccgcgccaa cagttgggcg tagtagtagt tcacactcgc
      121 gacgtcgaag tccggctccc gcgcaaacgg ctcgcgcagg tagtgcatgc gatgggcccc
      181 gccctcgcca ccctccacga actcgacaat cgccaggatg aaatcctccg gcttgttgag
      241 cgagaaccgg atctcgttgt gcgtcacggt gagcgtctgc gcacccgcca ctcgtccctt
      301 gacctcgatg aaccgcagct tccccgttcc cggcacccgg ctctcgatgt cgtagcccac
      361 cttctcggtc tcgcgatcca cgggctcgaa gcccaggctg cgctccacct ccatgacgat
      421 ggcgcgggcg cgagccgcgg cggcttgttt gtccgcggcc gcgctcgccg gcgtttccgc
      481 ggaaacgtgt attatcggaa gtagggcact gtcggccgcc accaccggct cctccccctt
      541 catcgcccgc accagcccga ccggcaccac gacgaacccg cccaggatca cgggaggcag
      601 gggagcgatc tgcgcctcca gcttcagctc ctcgatgcgc ttctgcaatc gtccctggag
      661 cagatcggcg cgctttcggg cccccatgga gttcaggcga gcgttcggtt tgccggcctg
      721 ctcctgagcc ttcagctctt ccgcgcgatg gtcccagtag ttgatctcct tgccgagccg
      781 atccttcacg gctgcctcgg tcttggcgat ccgctcgagc ctgccggtct tcacctcctc
      841 cacgtgttcg ggcacgatgt gcatgatcgc gtaggactgc gccgaaccct ccagctctcg
      901 gctgatccag gcgcactccg gccgagccag aatggcctcg atgcatgggt caccctcggc
      961 caaggccgga agtcgaggta gcgggcgtgg tggatgtcct tgggtgcggc ctcgcgcatc
     1021 catctcgatg tggaagaccc gcttggagac cacgcgccgc tcgccagacc tggtcgtgct
     1081 ggcatcctgg attggactct caatatagaa gaagatccgc ggctcggtgc tggaatcgcc
     1141 ctcatccacc aggaccgtgc ctcgcttcag caggtcgcgg ttgcgctcca gcgtcaggtc
     1201 gatcacggag tccaggagcg gggacccggg acacacgaag gtggccagcg gctgccccgg
     1261 cggggcgatc aggtcctttt cgaaggtggc tcgctcgtag tgggagagca cggcctctcg
     1321 ggtgccgatc agccggtccc ggctacggac gatggccggc acgtgcgtga tctcgtagcg
     1381 acgcggctcc cgctgccgca tggtcccacc gagtcgactg aacgcctcga tgaagaagga
     1441 ctcgatgaag tgcggttgaa gcctgcgagc ctcggcccgc tccatgtcct ctcggatctt
     1501 tcgaacccga ctgacgtcca tggagtcgtg caccagggcg tgctcctcca gcaggccctg
     1561 cagcttgcgt cggtccaccg cgttctccac cacccggttg agcctctcgc ggacctctgg
     1621 ccgctccccc tccctcaccg cctggatgag cagctcccgc agggacttgc cctcgaactc
     1681 cagcttgccc agcacgtcga agacctggcc gcccagcgcc ttgcgcgcct cgtcgagctt
     1741 ctccagcagc ctgtggtaca cgtcgccttc acgcgtctcg gtggcaacca ggttccaaag
     1801 gaagcagacc tcggtctggc cgattcgatg gatgcggccg aagcgctgct ccagccggtt
     1861 ggggttccag ggcaggtcgt agttcaccat caggtgggcg cgctggaggt tgatgccttc
     1921 ccctgctgca tccgtggcca ccagcacctg cacctcgggc tgaaacttga acgcctcctg
     1981 gttcgaaagg cgctcctcgc gccccattcc gccgtggatc gtgaccaccg cgtcttcccg
     2041 acccaggagc gtggtgatgc gcaggcgcag gtagttcagc gtgtcgcgat gctcggtgaa
     2101 gatcacgatc ttctgtctgg gcgatggctt gggcctcggc tccttgtccg ccgcgtccgt
     2161 cgagcccgga gcgccggaca gcagctccga cagcagaccg gcaagctgct tccacttcgt
     2221 gtcgttgccg ctccttcgga ccatgcccgc cagcgcctcc aggtgcctca gcgtgacgat
     2281 ctctgccctg agctctacga ttgtcctggc ggcagtagcc tggtccagga cctcttcctc
     2341 ggcctgctcc acctcggagt ccggcgcttc ctccaggtct tccagatcgt ctgggtccag
     2401 gcttcggcct ccgccaaggc cttcaccaac cgcaaagccc ctctggatca gctccagctc
     2461 cttcagcttg ctctccaggc gctcccggcg cctgagcagg gactggtaga tggcttctgg
     2521 cgatgatgcc agccgccgct gcagaatcgt gagcgcgaac ccgaccgttc ccgctcgctt
     2581 gtcgttctgc agcgcctctg cgcggctgaa ctcctcccga acgtaggagg tcacttccgc
     2641 gtacagctcc gcctctgcct ctgagagctt gaaggggacg gtcttcgcga tgcgctcggg
     2701 gaagagcggc cgcccgtcga acttcaggag ctgctccttg accattcgtc gcatcaggtc
     2761 gctggcatcg cttgtgtgca ccccatccct gaacttgccc tcgaagcgat cgccatcgag
     2821 cagggacatg aaaagctgga agtcctcctc cttgccgttg tgcggggtgg cagtcatgag
     2881 gaggaagtgt cgcgtcaggg tggacagcac gcggcccagt tggaagcgct tggtgtagct
     2941 gacctctccg ccgaagaagg tggcggacat cttgtgcgcc tcgtcgcaga ccaccaggtc
     3001 ccaccggcag tcaggcgcag ctaacttctg ctgaagctcg tcatcacggg cgagcttgtc
     3061 cagcctggcg atgatctggt ccgcttccag aaaccagttg cccgttcggg aggcctccag
     3121 gcggtcgttg gtcaggatct cgaagggcag gtggaaccgc tggttcatct cgtcctgcca
     3181 ctgctcggcc aggctcccag ggcagacgat caggcagcgc tgcaggtctc cgc
//
LOCUS       DASRZS010000294         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3244837, whole genome shotgun sequence.
ACCESSION   DASRZS010000294 DASRZS010000000
VERSION     DASRZS010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3244837"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VFH83_07250"
     CDS             complement(<1..749)
                     /locus_tag="VFH83_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HET6486199.1"
                     /translation="MTRSAPAFELYQVDAFTDTPFKGNPAGVCLLSHQRPDDWMMGLA
                     AEMNLSETAFVREQSDGYGLRWFTPKTEVSLCGHATLASAHVLWEEGIVASGTEIRFQ
                     TLSGTLPAHQDGKLIELDFPTKEVEPAPTLPDLNKALGVTPTFTGAYEREKGSTYLLE
                     VGSEEAVRAIEPDYARLVATGGRTVIVTARSSDGDFDFVSRFFAPVVGINEDPVTGSA
                     HCYLAPYWGRKLGKRDMIGHQVSARSGVVRCR"
     gene            complement(746..1543)
                     /locus_tag="VFH83_07255"
     CDS             complement(746..1543)
                     /locus_tag="VFH83_07255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET6486200.1"
                     /translation="MSTPQDYASISDASKPNAGRIYDYMLGGSHNFEVDRQAAQAILN
                     MIPGAPNMVRLVRWFLGEAVRRMCDAGFRHFVDFASGLPTVDHIHYVAPAGSKVIYSD
                     IDPVTVAYGQDIVKDNPNIRYLACNAATPEILLQSKDLTELMGKQRKVTVGFNGIAWF
                     LPDDQVAHALRTAYDWAAPGSRLFLTDNDVSSLNPQMRELMEIYKNMGQPSFPRSVKT
                     LKELAQPWKVSEPGPLALEEWVGIGKRVTNEIQNIIGTGIVGCIFEK"
     gene            complement(1608..>2418)
                     /locus_tag="VFH83_07260"
     CDS             complement(1608..>2418)
                     /locus_tag="VFH83_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6486201.1"
                     /translation="ALMAYHKGACIIGFTPSFVPFRVLFQFVMLLVRVVDWLLYRIQI
                     EGRENLRDSCRAVLVSNHTLVLDPGVIAHAIRPRRTYFTMLEETALIPYLGTFVRLLG
                     GIPIPESAGALRSLGTAADTALRTLGLIHFFPEGECYQWSQELAPFHPGAFLLACRLR
                     LPVIPITTVLRERRWFGRSSFRVGRRTIRLLPLVKVVIGEPVDPRSAGALAAGSLRGS
                     ARELAERVRGTMQRTIDRERGSKSLYRGMLPRLVRQAAPDAELSSATQRPA"
BASE COUNT          440 a          786 c          773 g          419 t
ORIGIN      
        1 cggcagcgca cgacccccga cctcgcggac acctggtgcc caatcatgtc tcgcttgccc
       61 agcttcctgc cccagtaggg ggcaaggtag cagtgcgcgg atccagtcac ggggtcttcg
      121 ttgatcccga ctaccggggc gaagaaacgc gagacgaagt cgaagtcacc gtcggaggac
      181 cgcgccgtca cgatgacggt ccgccccccc gtggcaacca gccgcgcgta gtccggctcg
      241 atggcccgga cggcttcctc ggatcccact tccaggagat aggtgctccc cttctctctc
      301 tcataggcac ccgtgaacgt gggggtgacc ccaagggcct tgttcagatc cgggagcgtc
      361 ggggccggct ctacctcttt cgtcgggaaa tccagctcga tgagcttccc atcctggtgt
      421 gctgggagtg tcccgctcag cgtctggaag cggatctccg tccctgacgc cacgatcccc
      481 tcttcccaga gtacgtgcgc gctggccagg gtggcgtggc cgcaaaggga tacttccgtc
      541 ttgggagtga accagcgcag cccgtagccg tcgctttgct cccgcacgaa cgcggtttcg
      601 gacagattca tttcggccgc cagacccatc atccagtcgt cgggcctctg gtgagacaaa
      661 aggcacacac cggcgggatt gcccttgaac ggagtgtccg tgaatgcgtc tacctgatag
      721 agctcgaagg ccggcgccga tctggtcact tctcgaagat gcaccctacg atgccggttc
      781 caatgatgtt ctggatctcg ttcgtgacgc gcttgccgat ccccacccac tcctccaggg
      841 cgagcggccc gggctcggac accttccagg gctgcgccag ctccttgagg gtcttgacgc
      901 tccgaggaaa ggacggttga cccatattct tgtagatctc catcaactcg cgcatctgcg
      961 ggttcaggga ggagacgtcg ttgtccgtca ggaaaagccg ggatccagga gccgcccaat
     1021 cgtacgcggt cctcagggca tgggccacct gatcatccgg gaggaaccag gcgatgccgt
     1081 tgaagcccac ggtcaccttg cgctgcttgc ccatgagctc ggtcaggtcc ttggactgca
     1141 acaggatctc cggcgtcgcc gcgttgcagg ccaggtaacg gatattcggg ttgtccttta
     1201 cgatgtcctg cccataggcc acggtcaccg ggtcgatatc ggagtagatg accttggatc
     1261 cggccggcgc cacgtagtgg atgtggtcga ccgtcggcag gccagaggcg aagtccacga
     1321 agtggcggaa ccccgcgtcg cacatccgcc gcaccgcctc tcccaggaac cagcggacca
     1381 ggcggaccat gttgggcgcg cctggaatca tgttcaggat ggcctgggcc gcctggcggt
     1441 ccacctcgaa gttgtggctt cccccgagca tgtagtcgta gatcctgccg gcattgggct
     1501 tactcgcgtc agagatgctg gcgtaatcct gcggtgtgga catcggatcg ctccttacct
     1561 gtattatgtg agaaaaagga agtctggcaa ggacgatttt cggcccgtta cgccggccgt
     1621 tgggtggccg aggagagctc cgcgtccggc gccgcctgtc gaaccagccg cgggagcatg
     1681 cctcggtaca ggctcttgga gccacgctcg cgatcgatcg tccgctgcat ggtgccgcgc
     1741 acgcgctcgg caagctcccg ggccgatccc cgcagcgagc ccgcggccag ggccccggcc
     1801 gaccgcggat ccacgggctc gccgatcacc accttcacca gcggaagcag gcgaatggtc
     1861 cgtcgaccca cgcggaagga ggaccggccg aaccatcgcc gctcacgcag aaccgtggtg
     1921 atgggaatca cgggcaaccg aagcctgcac gccaggagga acgctcccgg gtggaacggc
     1981 gccagctcct ggctccactg atagcactcg ccctcgggga agaagtggat gagccccagc
     2041 gtcctaaggg ccgtgtcggc cgccgttccg agggaccgca gggcgcccgc actctcgggg
     2101 atcgggatgc cgcccaacag acgaacgaac gtgcccaggt acgggatcag cgcggtctcc
     2161 tccagcatcg tgaagtaggt gcgtcgggga cggatggcat gggcgatcac gccgggatcg
     2221 aggaccaggg tgtgattgct caccagcacc gcgcggcaag agtctctcag gttctcccga
     2281 ccctcgatct ggatccgata cagaagccag tcgaccacgc gcaccaggag catcacgaac
     2341 tggaagagga cccggaaggg gacgaaggaa ggagtgaacc cgatgatgca cgcgcctttg
     2401 tggtatgcca tcagtgca
//
LOCUS       DASRZS010000295         3593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3628446, whole genome shotgun sequence.
ACCESSION   DASRZS010000295 DASRZS010000000
VERSION     DASRZS010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3628446"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFH83_07265"
     CDS             complement(<1..801)
                     /locus_tag="VFH83_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6486202.1"
                     /translation="MESARQICIIDNERNASVIGDFLQANGCAVARCTSCGAECRQEG
                     RHPDAILLNGDDLSDGRGALEAIRNREHLFQVPLIVYGARGERARMVKLLQRGATDYL
                     NAPLLKAELLEKIRVHTRTQALLRSFSRFVPEVYLRMMKKKDLANVALGDFAVVPLSV
                     LFTDVRSYTELSEKLAPQDIFRFLNSLFSRMEPVIKRNHGFVDKYIGDAILALFPGPT
                     DDCLDAGLNMMSELRRYNEHRASSGYPPVQMGLGMHQGDVALGTIGSDD"
     gene            1006..2274
                     /locus_tag="VFH83_07270"
     CDS             1006..2274
                     /locus_tag="VFH83_07270"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HET6486203.1"
                     /translation="MKGLKAGLSEVVITPPVGVRLSGYADRTGPSTGILDELYAKALV
                     LDNGERRLALVVCDVLGLSRSICEAIRDRVQGRTGIDGSDVLVAATHTHCGPDIDRLP
                     EASLDHLVAQAGGAVWAASRSLREANLGFGEGTCLAGVSRRNPRSPRVPYHLYSYPQG
                     TMDPRVLVMTVRGAQGERLGAVVNYPCHPVTLGWEEMNLSKDFVEYTCAVLKGAWGPD
                     AVPIFLQGCAANVNPRWVYDKPDADPILPPDWPQALEDRLRETRRLGHLLGGEALKAA
                     ETNTRYEAAVVLDARRVEVELPARSDLDLSDDAEESRPAGRYPGLRNRLRAQPRGFTT
                     EVQVLRMGDAFLIGLPGEVFVEYQIELRETLAGHRVFVSELAGDSIGYLPTPAAVIEG
                     GYEPTATYAVPEAGAALVQAALRAAREMVR"
     gene            2271..>3593
                     /locus_tag="VFH83_07275"
     CDS             2271..>3593
                     /locus_tag="VFH83_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HET6486204.1"
                     /translation="MTSLFYKPQDAWAADFIPLYSRGTYQLFYLLDWRDRDRHGEGTP
                     WYRISTRDFVSFQEHGEMLSRGTAAEQDLYVFTGSVLEAEGAFHIFYTGHNPHLRKAG
                     KPEQGVMHAVSSDLASWRKVPEDTFYAPAGRYEAHDWRDPFVFWNEEAGEYWMLLAAR
                     GTAGPPRRRGCTALCSSRDLRRWEVREPLWSPGLYFTHECPDLFRMGDWWYLLYSTFT
                     ERFVTHYRMSRSLRGPWLVPESDTFDGRGLYAAKTAGDGNRRFLFGWNATRDKESDTG
                     AWQWGGSLVVHELTQRPDGALAVKIPETVDRAFGPAVPVRFVSGLGRWKAHGNAVAIT
                     GTDSWGCVSAGALPPACRISATVRFSAGTQGCGILLRTSDDLETGYYVRLEPARRRLV
                     MDSWPRPGDKPFWLELERPVPLDAGSPHRIVVLVEGSICEVYLDDRVAM"
BASE COUNT          566 a         1159 c         1254 g          614 t
ORIGIN      
        1 atcgtcggaa ccgatcgtgc ccagggcgac gtcgccctgg tgcatcccga gccccatctg
       61 gacgggcggg taccccgagc tggcgcggtg ctcgttgtag cgccgcagct cgctcatcat
      121 gttcagcccg gcgtccagac agtcgtcggt ggggccggga aagagggcca ggatggcgtc
      181 ccctatgtac ttgtccacga agccgtggtt gcgcttgatg accggctcca tgcgggaaaa
      241 gaggctgttc aggaaccgga agatgtcctg gggcgcgagc ttttcggaga gctccgtgta
      301 ggagcgcacg tcggtaaaga gcacggagag gggcaccacg gcgaagtcgc ccagggccac
      361 gttggcgagg tccttcttct tcatcattcg cagatagacc tcgggaacga accggctgaa
      421 cgagcgaagg agcgcctggg tccgcgtgtg gacccggatc ttctccagga gctccgcctt
      481 cagaagcggc gcgttgagat agtccgtggc cccgcgctgc agcagcttca ccatccgggc
      541 gcgctccccg cgggcgccgt agacgatgag aggaacctgg aacagatgct cccgattccg
      601 gatggcctcc agggcccctc tcccgtcgct cagatcatcg ccgttgagga ggatggcgtc
      661 ggggtgacgc ccctcctgcc ggcattccgc tccgcagctc gtgcaccgcg ccacggcgca
      721 gccgttggcc tggagaaagt cgccgatcac ggacgcgttc ctctcgttgt cgatgatgca
      781 gatctgcctt gcgctttcca cgtgcgtgct ccggttctgg tgctcgaagc taacgtatcg
      841 cgcggtcccg cggagttcca aagggttgcc gactttgttc gctttttccc gtagccgcag
      901 gccagaccat ggcgcccccg ccgcctccgg atcatcccgg agccttgccg gggagggaag
      961 aacgaacctt tttggtatat tggagaccgg tcgcaggagg gcgtcgtgaa gggactcaag
     1021 gcaggtctgt cggaagtcgt gatcactccg ccggtcggag ttcggctctc gggatacgcc
     1081 gacaggacgg gaccatcgac ggggatcctg gacgagctgt acgccaaggc cctcgtgctg
     1141 gacaacgggg agcgtcggtt ggcgctggtg gtctgcgacg tcctgggtct gagccgctcc
     1201 atctgcgagg ccatccggga ccgtgtacag gggcggaccg gcatcgatgg gtcggatgtc
     1261 cttgtggctg ccacgcacac gcactgcggc ccggatatcg atcggctgcc ggaggccagc
     1321 ctggaccacc tggtggcgca ggccggaggc gccgtgtggg ccgcctcgcg ttcccttcgc
     1381 gaggcgaacc tggggttcgg cgaaggaacg tgccttgcgg gcgtcagccg gcgcaacccg
     1441 cgctctcccc gcgttccgta tcacctgtac tcctatccgc aaggaaccat ggatccacga
     1501 gtcctcgtga tgacggtccg cggagctcag ggcgagaggt tgggcgccgt ggtaaactac
     1561 ccctgccatc cggtgacgct gggctgggaa gagatgaacc tttcgaagga cttcgtggaa
     1621 tacacctgtg ccgtgttgaa gggcgcctgg ggtccagatg ccgtccccat cttccttcaa
     1681 gggtgcgctg ccaacgtcaa cccacgctgg gtgtacgata aaccggatgc ggaccccatc
     1741 cttccgccgg actggccgca ggctcttgag gaccgactcc gcgagacgcg gcgtctcgga
     1801 cacctgctcg gaggcgaggc gctcaaagcg gccgagacca acacccgcta cgaggccgcc
     1861 gtggtcctgg atgcccgacg ggtggaggtc gagctcccgg cgcgctcgga cctcgacctt
     1921 tcggacgacg cagaggagtc gagacccgca gggcgctacc cggggcttcg gaaccggttg
     1981 cgggcccagc cgcgaggctt cacgacggag gtccaggtac ttcggatggg ggacgcattt
     2041 ctcatcgggc tccccgggga ggtgttcgtg gagtaccaga tcgagctccg ggagacgttg
     2101 gcgggacacc gggtgttcgt atcggagctt gccggagact ccatcggcta cctccccacg
     2161 ccggccgccg tgatcgaggg agggtacgag cccaccgcca cctacgctgt tcccgaggca
     2221 ggcgcggccc tggtccaggc ggcgctccgc gccgcgaggg agatggtccg atgacgagcc
     2281 tgttctacaa gccccaggat gcgtgggcgg cagacttcat cccgctgtac agccgcggga
     2341 cctaccagct cttctacctc ctggactgga gggaccgtga ccggcacggc gaggggacgc
     2401 cctggtatcg gatcagcacc cgggacttcg tgagcttcca ggagcatggg gaaatgcttt
     2461 cccgcgggac ggcggcggag caggatctgt atgtgttcac cggcagcgtg ctggaagcgg
     2521 agggcgcctt ccacatcttc tacacgggcc acaaccctca cctgaggaag gcggggaaac
     2581 ccgagcaggg agtgatgcac gccgtaagct cggacctggc ctcgtggcgc aaggtgccgg
     2641 aggatacctt ctacgcgccc gccggtcggt acgaggcgca cgactggcgc gacccgttcg
     2701 tgttctggaa cgaagaggcc ggcgagtact ggatgctcct ggctgcccgc ggcacggccg
     2761 gccccccgcg ccggcgcggg tgcacggcgc tgtgctcgtc gcgggacctt cggcgctggg
     2821 aggtccgaga gccgctctgg agcccgggcc tctacttcac gcatgaatgc ccggacctct
     2881 ttcggatggg cgactggtgg tatctcctct actccacgtt caccgagcgg ttcgtcacgc
     2941 actaccggat gagtcgatcc ctgaggggcc cctggctggt tcccgagagc gacaccttcg
     3001 acggaagagg cctgtatgcg gccaagaccg cgggagatgg aaaccgccgg ttcctgttcg
     3061 gatggaacgc cacgcgggac aaggagtcgg acacgggcgc ctggcagtgg ggcgggagcc
     3121 tcgtggtcca cgagctgacg caacggccgg atggcgcgct cgccgtgaag atccctgaaa
     3181 cggtggatcg cgccttcggt ccggcggtgc cggtccggtt cgtctccggc ctggggcggt
     3241 ggaaggccca cggaaacgcg gttgccatca ccggcaccga ctcctggggc tgcgtgtccg
     3301 cgggcgcgct cccgccggcg tgccgcatct ccgccacggt ccgattctcc gcgggcaccc
     3361 aggggtgcgg gatcctgctt cgaacgagtg acgacctgga gaccggctac tacgtgcgcc
     3421 tggagccggc gcgtcggcgc ctggtcatgg attcctggcc ccggccggga gacaagccct
     3481 tctggctcga gctcgagcgg ccggtgccgc tggacgccgg ctctccccat cggatcgttg
     3541 tcctcgttga gggctcgatc tgcgaggtgt acctcgacga tcgagtggcc atg
//
LOCUS       DASRZS010000296        15538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4785538, whole genome shotgun sequence.
ACCESSION   DASRZS010000296 DASRZS010000000
VERSION     DASRZS010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 15538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15538
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4785538"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFH83_07280"
     CDS             complement(<1..509)
                     /locus_tag="VFH83_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486205.1"
                     /translation="MSTFATVLWKDLRVRFSSRAELVFFIVLPVVFIAVLSGTMGTSP
                     TLGERAPLVLVYDTVHSGPSSGMFSALAAVPGLQAREVDDPTELIEKADADLLLWLTP
                     STTTDKPFTVTVRLSPWRSSAAQSAKRVEAALGGARVEAGGSDARSGTGPRPPSSAGV
                     PSDAADANAG"
     gene            complement(509..1267)
                     /locus_tag="VFH83_07285"
     CDS             complement(509..1267)
                     /locus_tag="VFH83_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486206.1"
                     /translation="MSVVLIAMQELKRVLRDPSGLLLTLALPLALTVAVGFISGRVPL
                     GRPPSDGFDALSYVAPGMALFAMMFSVRQAAKTTVDDADRGITRRLRSAPIGEISIAV
                     GRTLSGVVMVAAQLLLLVGVSSALYGLRWGSPLPVLLLCLALSLSAGGWAALLVSLGR
                     TSRGVGTLGTALTLVFAILSRSFAAVVPSAPWMDALSRITPNHWGLHGFSALALSGTL
                     GSIAPDLLRLVLMSVTLWILAPLAGRLMTWRRKT"
     gene            complement(1264..2217)
                     /locus_tag="VFH83_07290"
     CDS             complement(1264..2217)
                     /locus_tag="VFH83_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486207.1"
                     /translation="MSLVEVNDLGKSYGSLAAVQGLTFQIEEGEVFSLLGPNGAGKST
                     TIGMLCGLVDPSAGDARIGGISIRGRSRQLRRTCGIVPQEIALYEELSVRDNLVFWGR
                     LYGLRGKTLGTRVQAALSRFGLADRATSVVRTLSGGMKRRLNIAAGILHGPRLVFMDE
                     PTVGIDPQSRRAILDLILELKAQGSTVLYTTHYMAEAEELSDRVGIIDHGRLLAMGSC
                     KELAQGIGGQESVLLQLGNEEDGGRLVQELEGMPGVKAASDGSRVTVAAARVSQILPR
                     IVEAAASIRASLRDIEIREPNLETVFLAYTGRALTASGGEA"
     gene            2345..3322
                     /locus_tag="VFH83_07295"
     CDS             2345..3322
                     /locus_tag="VFH83_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486208.1"
                     /translation="MSRMAIDPSRLTVPGNMLLLGEYAVLEEGGLGIAVAVERRVRFH
                     ARPSDRFRIEGVWPGGAMRWTGPQDGNPVITETVASVTSWAEHSGIPAHLPGATLRVD
                     STELFDASGRKAGFGSSAAVTVGLTCQLTDLLLVRAARAPADADALVGLALESHRRAQ
                     GGVGSGYDVLSSFHGGVGAFRGGRHPTWEASRISGLLDLALFRGKAPISTADSVRRYQ
                     EWKRRDPAAAVELLERSNAAVSGFLAATTASMAFRWFSAAREIGLALGERIGVSAGIE
                     PPPGVDPAQCKAVGAGNELGVYVFEPGSRMPPGRELERLSVADRGLRRG"
     gene            3326..4183
                     /locus_tag="VFH83_07300"
     CDS             3326..4183
                     /locus_tag="VFH83_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HET6486209.1"
                     /translation="MTPVSVGDGELDAPVRELAAFLAGRRTVALSGAGMSTDSGIPDY
                     RGPNGSLRFRQPMQYREFTGSEAARRRYWARSYLGWDRIRAARPNPGHLALARIEKTG
                     SLVGLITQNVDGLHQAAGSLRVLELHGSLAEALCLDCGQREARDHLQDRLREANPEWN
                     PGIIAFAPDGDAELERQSESSFRVPPCLRCGGTLKPDVVFFGESVPRERVEAAWSLFS
                     EAERLLVVGSSLTVFSGFRFVERAVRLGMPVAIVNRGPTRGDPNAAIRIEGSLSEVLP
                     AVSEALASG"
     gene            complement(4200..4958)
                     /locus_tag="VFH83_07305"
     CDS             complement(4200..4958)
                     /locus_tag="VFH83_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET6486210.1"
                     /translation="MTVFAFSCHPDDIEFMMAGTLLLLKDAGCALHYMNLADGSCGGT
                     THDARQTARIRREEAMESARLLGAAFHPSLTRDIEVFYQDELIRRAAAVIREVAPDIL
                     LLPSPEDYMEDHMITARIGVTAAFCRGMPNYASIPPRAPIRKDVVLYHAMPAGLVDGL
                     RRAVQPDFYVDVEPAIDRKERLLACHRSQKLWLDESQGFDSYLQQMRDLSTRVGQMSG
                     RYRFAEGWRRHLHLGFSAQERDPLRELLARSTTA"
     gene            complement(4955..5872)
                     /locus_tag="VFH83_07310"
     CDS             complement(4955..5872)
                     /locus_tag="VFH83_07310"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6486211.1"
                     /translation="MSDEVGELRNSQDALSDAEELRRRLRRDGYIFLRGLLAREDVVE
                     LRRALLEVCQRHGWLSAGSQLLDARVSRPPTQEGSAEFMPVYVEIQKMEAFHRFAHQA
                     ALIDPIARLLDEPVFVHPMKIARISFPNNVQQTTPSHQDYVHIQGSFETYTSWIPLGD
                     VPRSLGGLAILAGSHKLGILQPRAAYGAGGLGLDVDSLGLPWHGTDYRAGDVLLFPST
                     AIHKALPNLSQDRLRLSVDYRYTGVTHAVGEGSLKPHYHWQVPELTYENIYRDWKSED
                     LKFYWKKLPLHVVPLTDAWAKMARGDDKR"
     gene            6009..6839
                     /locus_tag="VFH83_07315"
     CDS             6009..6839
                     /locus_tag="VFH83_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6486212.1"
                     /translation="MNPRIGVLASLKAEGSSFQPVLDLGLRVCQLSGWEPELWSDALA
                     ERVKQESTSSGVRMSAFWAGWPGPKVWDFVSGPATLGIVPKEYCEMRVRALQKAAGFA
                     SRLGVTAIITHLGFIPENPSDPAFAEVVSAVRRIAVRLQALGMEFWFETGQETPVTML
                     RLIRSVGTGNLGVNLDPANLILYGKGDPVDSLDVFGSLVRNVHAKDGLYPTDPMKLGI
                     EVRLGEGKVRFPQFIRRLAEVGFRGELIIEREISGDQQTRDIRQAALYLDGLLKEAGG
                     "
     gene            6836..7825
                     /locus_tag="VFH83_07320"
     CDS             6836..7825
                     /locus_tag="VFH83_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6486213.1"
                     /translation="MIRVGFIGTGNISATHLRYLASRDDVTIAALCDTDEQRLAGRSR
                     EFGGTPFSDFRRMLDAVALDAVWVCTPQRVRREPLLACADRGLAVFCEKPVEHNAEAA
                     AEISAELSRLNARVQVGYLFRSLPVVRRLREEMAGDTIRLVASYYGCSVGIDPKLPNW
                     FCEKEKSGGPLVDQATHNLDLLRSLFGEVVRVQGTANNPLHPKAPGYTIDEVITVGLR
                     FANGILGSHNHTWLGDGWRNEILLSGERRFYRLDLTRGRLTVEEAQDTRVFHQDATPI
                     HHYQDEVFLRELVTGTGAANPCTYADGLCTLRLTLACNAAVERGDGAAVAVIA"
     gene            7853..12103
                     /locus_tag="VFH83_07325"
     CDS             7853..12103
                     /locus_tag="VFH83_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET6486214.1"
                     /translation="MPAPPSIILLNDALKKTLLSMEESQKRRLREKLEFLEQGIWDSG
                     VKVKKLRGLSGKVIFEARVSRGDRILFTLGRHEGRAAVYLWAFTTHDDLDAAARRIQP
                     ANAPFLDFTPQSEEELPEVEIERLDSSYFSQEDLSTQVPDDCGPQKWLVLDEGEWRRL
                     LLSPDADRFEIFLCLTQEQEQVLLQDPPLLLSGTAGSGKTTISVYYLLHARYARSRRI
                     YLTFGPALADLARRMVSGLTDQREPIAGEQPPLILTFRELLFRIVQERSQEFPPEREV
                     QLAEFTRILATHPDARGYDSELVWEEIRSIVKGAKLPLNPGRFSRLVDGFLSRDLVPR
                     ERIELREYLAGLESLEVGAQVERFIQKRTGLGTYAGLLTRMQAAGAYADGAVAETLQE
                     AVRLVTRRASDFSSPLLSLEEYLALGRKRAPTFVADRRRIYGLALHYQERLGSGRLWD
                     EIDLCRAALQSLEESGHQGAYDLVVCDEVQDFTDVQVSLLFRLARDPRSVVLTGDPRQ
                     IINPSGFRWEEVKNKFYERGLPVPDLSRLSLNFRCAGPLVRLANALLEMKQAFVGTAD
                     TDAREQWKFGGRPPLLVSRASEEEILGELKTGSAGRIVLTRTEPERDRLKRALSTELV
                     FTIQDAKGLEFDTVLLWKFASEAASLPIWRGMQAGRPDPAQVPRIRHEISLLYVAVTR
                     ARNSLILYDGKAPSDVWTSPHLVEHLFRTEDTDRLAELWRVLSTPQEWERQGDYFFER
                     HYYPAARECFRNAGTVAKAEAAEGFLLARAGEHALAAPLLARNGHARQAAESYEAAGM
                     PQQAAELWDQVGETRRARLCRIRALEAAGQWSQAAAAWEQEGQRDRAFPCWEKAGNHQ
                     WVGEYLLSAGKIEPALKALERSGDVARQAECWRKLGKLERAADLYARTGDVARAISLY
                     REVGATEKLLRCLQRIGDYRGVAVEYERSGDWARAVDAYRRLAESSAPGRKALEDEIS
                     EQPRQRKTMIRSALRLSALGREREAGPLYRAAGLFEPAVRAFQAVQDYEQLAECLSLL
                     GRHREAAAAAEKSDLPDARREDLVFRHLERHLQADPRRESRTALELEEEADRLLAEGK
                     LVQALVRYRALGNGPGALEAYVRLGRDEEAILEFLAGGSPALARKYVEAKEINVSAEF
                     LRTVAMRPDPEEGTRQAAAPAERSPSERRQMLKALFRKALRRLPQAEAEDLLDECSEA
                     VFGFPILHEASGADWDWLLSMKQYNTIVQMVAYIPATRRGPSEAVRPFVSRLETEAMR
                     TGDPALLTCHALATDPDRVAEAASGVSLGARTVEVLGRCRTRYREAVELQLGRNNIDA
                     AIDYCRLAEDYGLAGSLHERMGEMREAINSFLLAKDYAAAMRCARASGKEPLVARVYE
                     RQGDLKAAVAVWRRLGRPREVERVLRRLEKSGGRNKEP"
     gene            12108..12980
                     /locus_tag="VFH83_07330"
     CDS             12108..12980
                     /locus_tag="VFH83_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET6486215.1"
                     /translation="MTVTASYGVIVLGTVLWDAGAILQKQAIEKLGSGRLRVVRLLGS
                     PRWMLGLAANAVGWGLFVLGLDSVPVSVARTVTGGSYVVLALFSTVFLRTPLKLPEWL
                     ALICVTAGIVILGAQGQAGRVTADTLLPSVIRLGLGLGAIIVVCGSTLLATRGAGSII
                     AFAATSGLLSGAGDLMVKLITILAREEARPLAGVGVALAALSGVGMVLFYVTGIYMLS
                     RAYQVGTVVAGMVISDFFARVAAIFLGAVCLSEPLVAAGRAGPLYAVGFLMVLGGSLL
                     LGRFSSRPVRIASS"
     gene            13013..13951
                     /locus_tag="VFH83_07335"
     CDS             13013..13951
                     /locus_tag="VFH83_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET6486216.1"
                     /translation="MKTFEVWAMGELLVEIMRPRRGMSLSEAGDFLGPFPSGSTGIFI
                     DTIARLGHSAAMISGVGDDDFGRCILERFRRDGVDCSRILVVPNQATGVAFVTYFPDG
                     SRRFLYHWDNTPAVMARRPDPDLIQEPRFLHVMGCALMPNDLFRAEVFETVKLFASKG
                     ARVSFDPNIRFELLGGRTAESIIGPVIEHSSVLLPGERELQLLSGIEDIDAAARRLLS
                     RPRMEIIVVKRGSRGCTVYSRDGRIDVPPYRVQEVDPTGAGDCFDAGFICGLLEGKSL
                     EESARVAAAAGALNAAAFGPMEGDISPRSIAAVQAR"
     gene            13965..15332
                     /locus_tag="VFH83_07340"
     CDS             13965..15332
                     /locus_tag="VFH83_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HET6486217.1"
                     /translation="MSLTSGDRPVSPLVEIVASQKRGEARGLGSVCSSLRPVLEAAVQ
                     QALEDGAPLLVESTCNQVNQFGGYSGLTPVAFVALVRSLANASGLPGARLVLGGDHLG
                     PYPFRGEPAQSAMAKAAEMVRAYIAAGYRKLHLDASMRLADDPGEPRGPLDVQRIAER
                     CADLCSAAEAAWAEARSEGGTDAEEPPVYVIGTDVPTPGGSNEVESAVHVTTASELQE
                     TIETTRRRFLAGGLVSAWERVIAVVVQPGVEFGDQAIVAYDRRRSGGLIEAVRKTPRL
                     VMEGHSTDYQTPRALRELVEDGVAILKVGPALSAAAREAVFLLAHVEEEICRLRAGLE
                     PSGIGDALEEAMIANPVHWQGHYTGDENQRSFARRFSLLDRVRYYWGVPEVERAMARL
                     FSNLRAAPIPLPLLSQYLPQQHGRIRAGALTNDPGAMLRDRIREVLRGYSWAAGFQPG
                     PKEGA"
BASE COUNT         2529 a         5019 c         5364 g         2626 t
ORIGIN      
        1 cccgcgttgg cgtccgccgc gtcggacggg actcctgccg aggatggggg gcgcggtccg
       61 gttccggacc ttgcgtcgga cccgcccgcc tcgacgcgtg ccccccccag cgcggcctcc
      121 acccgctttg cggactgcgc cgcggatgac ctccacgggg acagtcggac ggtaaccgtg
      181 aagggtttgt ccgtggtcgt cgaaggcgtg agccacagca gcaggtcggc atccgccttc
      241 tcgatgagct ccgtgggatc gtccacttct cgcgcctgaa gccctgggac cgccgccagg
      301 gccgagaaca tgccgctgct gggccccgag tggaccgtgt catagaccag gaccagcggc
      361 gcccgctcac ccaacgtagg ggacgtcccc atcgttcccg agagcacggc gatgaacacc
      421 acgggcagca cgatgaagaa caccagctcg gcccggctag agaaccgaac tcgcaggtct
      481 ttccagagaa ccgtggcgaa cgtactcatc aggtcttcct tctccaggtc atgagcctcc
      541 cggccagagg cgcaaggatc cagagcgtca cgctcatgag caccagcctc aggaggtccg
      601 gggctatgga gccaagggtt ccgctcaacg cgagcgccga gaaaccgtgc agcccccagt
      661 gattgggagt gatccgcgaa agcgcatcca tccacggcgc actggggacg accgccgcga
      721 agctgcggct caggatggcg aagaccaggg tgagggcagt ccccagcgtc ccgactcccc
      781 gcgacgtgcg acccagggaa accaggagcg ccgcccaccc gccggccgaa agcgacaacg
      841 ccagacagag gagcaggacg ggcagaggag atccccaccg caggccgtac agcgcgctcg
      901 agacaccgac caggaggagc agctgggcgg cgaccatgac cacaccggag agggtgcgcc
      961 cgaccgcgat gctgatctct ccgatgggag ccgaacggag ccgacgggtg atcccccgat
     1021 ccgcgtcatc caccgtggtc tttgctgcct ggcgtaccga gaacatcatc gcgaagagcg
     1081 ccatccccgg tgcgacgtac gacagggcat cgaagccgtc cgagggtggc cgtccgagcg
     1141 gcacgcggcc cgagatgaag ccgacagcca cggtcagggc cagcgggagc gcaagggtca
     1201 ggaggagccc cgacggatcc ctcaggactc tcttcagctc ttgcatcgcg atgaggacga
     1261 cgctcatgcc tcacctcccg aggcggtcag ggcgcgtccc gtgtaggcca ggaagaccgt
     1321 ctcgaggttc ggctcccgga tctcgatatc gcgaagcgac gcccggatcg aggcggccgc
     1381 ctccacgatc cgcggcagga tctggctcac gcgggccgcg gccaccgtca ctcgggaacc
     1441 gtcgctcgcc gccttcacac ccggcatgcc ctccagctcc tggaccaacc ggccgccgtc
     1501 ctcttcgttt cctagctgca gaaggacgga ctcctgaccc ccgatcccct gagccagctc
     1561 tttgcagctg cccatggcca ggagcctgcc gtggtcgatg atcccgaccc ggtcggagag
     1621 ctcctcggct tccgccatat agtgcgtcgt gtagaggacc gtggaaccct gggccttcag
     1681 ctccaggatc aggtccagga tggcacgacg gctctgaggg tcgatcccca cggtcggctc
     1741 gtccatgaac accagccgcg gaccgtgcag gatgccggcc gcgatattca agcgacgctt
     1801 catgccgccg gagagcgtcc gcaccacgct ggtcgcccga tccgcaaggc cgaagcgcga
     1861 gagggcggcc tggacccgcg tacccagcgt cttgcctcga agtccgtaca gcctccccca
     1921 gaagaccagg ttgtcccgga ccgaaagctc ctcgtacagc gcgatctcct gtgggacgat
     1981 cccgcatgtc cgccggagct gccggctcct tccccggatg gagatgccgc cgatgcgggc
     2041 atccccggcg ctggggtcta ccagcccgca gagcatgccg atggttgtgc tctttccggc
     2101 tccgttcggg cccagcaggc tgaacacctc tccttcttcg atctggaagg tcaacccctg
     2161 taccgccgca aggctcccgt aactcttccc gaggtcgttg acctccacca ggctcatgat
     2221 cgcctcctcc gagatgctct caatgtgctc gaccggcccc ccgagcagga agtgccgaag
     2281 gtcatgaaaa ggtcacatga cccgctcctt tttcggccac ccgccggggt atagaatctc
     2341 ggcaatgtcc aggatggcga tcgacccgtc tcggctgacg gtgccgggga atatgcttct
     2401 cctcggggaa tacgcggtac tcgaggaggg gggcctcggg atcgccgtag cggtcgagcg
     2461 gagggttcgc ttccacgcga ggccatccga ccgctttcgg atcgagggcg tatggccggg
     2521 cggagccatg cgctggacgg ggccccagga cggcaacccg gtgatcaccg agaccgtggc
     2581 cagcgtcaca agctgggcgg agcactccgg gatccccgcg cacctgccgg gcgcaaccct
     2641 gcgcgtcgat tccaccgagc tttttgacgc gagcggccgc aaggcgggct tcggatcgag
     2701 cgcggcggtg acggtagggc tcacatgcca gctcacggat ctgctcctgg tccgtgcggc
     2761 ccgcgcgccg gcggacgcgg acgcgcttgt cggcctggcc ctggaatcac accgacgggc
     2821 tcaggggggc gtgggaagcg gctatgacgt gctgagctcg tttcatggcg gcgtgggagc
     2881 tttccgagga gggcgccatc cgacatggga ggcgagccgt atcagcgggc tcctcgatct
     2941 cgccctgttt cgaggaaagg cgccgatctc cacggcggat tcggtgagac ggtaccagga
     3001 atggaagagg cgcgaccccg ccgcggccgt ggagctcctg gagcggtcga acgcggccgt
     3061 ctccggtttt ctggctgcaa ccacggcatc catggccttt cggtggttct ccgccgcgcg
     3121 agagatcggt ttggccctcg gcgagcggat cggagtgtcg gccggtatcg agccgccgcc
     3181 gggtgtggac ccggctcagt gcaaagcggt cggggctgga aacgagcttg gggtctacgt
     3241 cttcgaaccg ggctcacgga tgcccccagg tcgtgagctg gagcgcctga gcgtggccga
     3301 ccgtggtctt cggcgggggt gagcgatgac cccggtatcg gtgggtgacg gggagctgga
     3361 cgcaccggtt cgggagcttg ccgcattcct ggcgggccgg agaaccgttg cgctgagcgg
     3421 cgccggtatg agcacggact ccggcattcc cgactaccgc ggccccaacg ggtccctccg
     3481 gtttcgccaa cccatgcagt accgggagtt caccgggagt gaggcggcgc gccggcgcta
     3541 ctgggccagg agctatctcg gatgggaccg gatccgcgcg gcccggccca accccgggca
     3601 tctggcgctg gccaggatcg agaagacggg ctccctggtt ggtctcatca cgcagaacgt
     3661 cgacgggctg caccaggcag cgggaagcct ccgggtgctg gagctgcacg gatcccttgc
     3721 cgaagcactc tgcctggact gcggccagcg ggaggctcgg gaccacttgc aggaccggct
     3781 ccgagaggcg aatccggagt ggaacccagg catcatcgcc tttgcgccgg acggggacgc
     3841 ggagctcgag cgtcagagcg agtcctcctt ccgggtgccc ccgtgtctgc gctgcggcgg
     3901 aacgctgaag ccggatgtcg tgttcttcgg tgagagcgtg cccagggaac gcgtggaggc
     3961 ggcgtggtcc ctgttctccg aggccgaacg gctcctggtc gtcggctcct cgttgacggt
     4021 gttctccggt tttcggttcg tggagcgcgc ggtcaggctg gggatgcccg tggccatcgt
     4081 gaaccgcggc ccgacgcgag gggacccgaa tgccgccatc cgaatcgagg ggagtctgag
     4141 cgaggtgctc cccgccgtat ccgaggccct ggcctccggc tgaggcgtca ctccctgcct
     4201 caagcggtcg tcgatcgcgc cagcaactcc ctgagaggat cccgttcctg ggcggaaaat
     4261 ccaagatgca ggtgtcgtct ccagccttcc gcaaaccggt agcgtccgga catctgtccc
     4321 acgcgggtcg acaggtctct catctgctgc aggtagctgt cgaacccctg gctctcgtcc
     4381 agccagagct tctggctgcg atggcaggca agcagtctct ccttgcggtc gatcgcgggc
     4441 tccacgtcca cgtagaaatc cggctgcacc gcgcggcgca gaccgtcgac cagccccgcc
     4501 ggcatggcat ggtagagcac cacgtccttc cggatcggtg cgcggggcgg gatggatgcg
     4561 tagttcggca ttccgcggca gaacgcggcc gttacgccga tacgggccgt gatcatatgg
     4621 tcctccatgt agtcttcggg ggaaggcagc aggaggatgt cgggagcgac ctcccggatg
     4681 accgccgcgg ctcgtcggat cagctcgtcc tggtagaaga cctcgatgtc ccgcgtgagg
     4741 ctcgggtgaa acgccgcgcc cagaagtcgc gcggattcca tggcctcctc acggcgaatc
     4801 cgggcggtct gccgggcgtc atgggtcgtc ccgccgcaac taccgtccgc caggttcatg
     4861 tagtgaaggg cgcaccccgc gtccttcagg aggagcaggg tgccggccat catgaactcg
     4921 atgtcgtccg gatgacagga gaacgcgaag acggtcatct cttgtcgtca ccgcgcgcca
     4981 tcttggccca ggcgtcggtg agcggaacga cgtgcagggg cagcttcttc cagtagaact
     5041 tcagatcctc gctcttccag tcccgataga tgttctcgta ggtgagctcg gggacctgcc
     5101 agtggtagtg cggcttcagg ctgccctcgc cgacggcgtg cgtgacgccg gtataccgat
     5161 agtccacgga cagacgaagc cggtcctggc tcaggttggg cagcgccttg tggatagcgg
     5221 tgctgggaaa gaggagcacg tcgccggccc ggtaatcggt gccatgccac ggcaggccga
     5281 ggctgtccac atccagtcca agcccgccgg cgccataggc ggcgcggggc tgcaggatcc
     5341 cgagcttgtg cgagccggcg aggatggcca ggccgccgag gctccgcgga acgtctccca
     5401 aggggatcca gctcgtatag gtctcgaagg aaccctggat gtggacgtag tcctggtgcg
     5461 acggcgtggt ctgctggacg ttgttaggga aggagatccg ggcgatcttc atggggtgca
     5521 cgaagacggg ctcatccagg agcctggcga tgggatcgat cagcgcagcc tggtgagcga
     5581 agcggtgaaa cgcttccatc ttttggatct ccacgtacac cggcatgaac tcggcggagc
     5641 cttcctgggt cgggggacgg ctgaccctgg catccaggag ttgggagccg gctgagagcc
     5701 agccgtgtcg ctggcagacc tccaggagcg ctcggcgaag ctccactacg tcctcccggg
     5761 ccagcaggcc ccggaggaag atgtacccgt cccgtcggag cctgcgtcgc agctcctcgg
     5821 cgtcgctcag ggcatcctga gagtttctca gctcaccaac ctcgtcggac atgctgactc
     5881 cttgccgcgg gcggtagagg ctccgcgggt tcaaacatcg gtaggacgcc gaggcccgtc
     5941 aaggggacgg ggcgggcctt gccccccctg ggaaaaagga gcacggtggt ttcttgagga
     6001 gcgttggcat gaatccccgg atcggtgtgt tggcctcgct gaaggcggaa ggatccagct
     6061 ttcaacccgt gctggacctc ggcttgcgcg tctgtcagct cagcgggtgg gagccggagc
     6121 tctggtcgga tgccctcgcc gagcgggtga agcaggagtc cacttccagc ggagtccgca
     6181 tgagcgcctt ctgggccggt tggcccggac ccaaggtgtg ggatttcgtc tccgggccgg
     6241 ccacccttgg gatcgtgcca aaggaatact gcgagatgcg ggtgcgcgcg cttcagaaag
     6301 ccgccgggtt tgccagccgt cttggagtca cggccatcat cactcacctg gggtttatcc
     6361 cggagaatcc ttcggacccg gcgttcgccg aggtggtctc cgcggtgcgt cggatagccg
     6421 ttcgactgca ggcgctgggc atggagttct ggttcgagac cggccaggag acgccggtga
     6481 ccatgcttcg gctcattcga agcgtaggga cgggaaacct cggcgtcaac ctggacccgg
     6541 ccaacctcat cctgtatggc aagggcgatc ccgtagacag cctggatgtc ttcggctctc
     6601 tggtccgcaa tgtccacgcc aaagacggcc tctatccaac ggaccccatg aagctgggca
     6661 tcgaggtaag gctcggggaa gggaaggtcc ggttcccgca gttcatccgt cgcctcgcgg
     6721 aggtggggtt tcggggtgag ctcatcatcg agcgggagat cagcggggac cagcagaccc
     6781 gcgacatccg gcaggcggcg ctctacctcg atggactgct gaaggaggca ggcggatgat
     6841 acgcgtggga ttcatcggga ccggcaacat ctccgcaacc caccttcggt acctggcctc
     6901 tcgtgacgat gtcaccatcg cggcgctttg cgacaccgac gagcagagac tggcggggcg
     6961 ctcccgggag ttcggcggca cgccattctc ggacttccgc cgcatgctgg acgcggtcgc
     7021 gctggatgcc gtgtgggtct gcaccccgca gcgcgtacgg cgcgagccgc tcctggcgtg
     7081 cgccgaccgg gggctggcgg tgttctgcga gaaaccagta gagcacaacg cggaggcagc
     7141 cgcggagatc tccgcagagc tttcgaggct gaacgcccgg gtccaggtgg gctacctctt
     7201 tcgttccctt ccggtggtgc gtcgactgcg ggaggagatg gcgggagata ccattcggct
     7261 cgtagcctcg tactacggct gttccgtcgg catcgacccc aagcttccca actggttctg
     7321 cgagaaggag aagtcggggg gccccctggt ggaccaggcc acccacaacc tggatctgct
     7381 tcgcagcctc tttggagagg tcgttcgcgt tcaagggacc gccaacaacc cgctccatcc
     7441 caaggccccg gggtatacga tcgacgaggt gatcacggtc gggctcaggt tcgccaacgg
     7501 catcctcggc tcccacaacc atacctggct gggggacggc tggcggaacg agatccttct
     7561 gagtggggaa cggcggtttt accgtctgga cctcacccgg ggccgcctga cggtggagga
     7621 ggcgcaggac acccgagtct ttcaccagga tgccaccccc atccatcact accaggacga
     7681 ggtctttctt cgggagctcg tgacgggtac gggcgccgca aacccgtgca cctacgccga
     7741 cggtctgtgt accctgcggc tcacgctcgc ctgcaatgcg gccgtggagc gcggcgacgg
     7801 agcggcagtc gccgtgatcg cctgaagcgt aagcggaccc gagcggccac cgatgccagc
     7861 gcctccctcc atcatcctgc tcaacgacgc cctgaagaag accctcctct ccatggagga
     7921 gtcccagaag cggcgtttgc gggagaagct ggagtttctg gaacagggga tctgggactc
     7981 cggcgtcaag gtgaagaagc tgcgcgggct ctccgggaag gtcatcttcg aggcgcgcgt
     8041 cagtcggggc gatcgcatcc tctttacgtt gggcagacac gagggtcgag ccgcggttta
     8101 tctctgggca ttcaccacgc acgacgacct ggacgccgcg gcccgccgca tccagccggc
     8161 aaacgctccg tttctggact ttacgcccca aagcgaggag gagctgcccg aggtcgagat
     8221 cgagcgcctc gactcctcgt acttcagcca ggaggacctc tccactcagg tcccggacga
     8281 ctgtggaccg cagaagtggc tggtcctgga cgaaggggag tggagaaggc tgctgctctc
     8341 accggacgcg gatcgcttcg agatcttcct ctgtctcacg caggagcagg agcaagtgct
     8401 gctccaggat ccgccgcttc tcctgtcggg taccgccggc agcggcaaga cgaccatctc
     8461 cgtctactac ctgctccatg cccggtacgc gcggagtcga cggatctacc tgaccttcgg
     8521 tccggcgctg gccgacctgg ctcggcgcat ggtgagcggt ctgacggacc agagggagcc
     8581 catcgcgggc gagcagccgc ccctcatcct gacgtttcgc gagctgctct tccggattgt
     8641 ccaggagcga tcccaggagt tcccgccgga acgggaggtt cagctagcgg agttcacccg
     8701 gatcctggcc acccatcccg acgctcgagg gtacgattcc gagctggtct gggaagagat
     8761 ccgttccata gtaaaaggcg ccaagctccc gttgaacccg gggcggttct cccgcctggt
     8821 ggacgggttc ctttcccgcg acctggtccc tcgcgagcgc atcgagctgc gggaatacct
     8881 cgccgggctg gagtcgctgg aggtaggggc ccaggtggag aggttcattc agaagcggac
     8941 cggtctaggg acctacgccg gacttctgac ccggatgcaa gccgcggggg cttacgcgga
     9001 cggggcggtg gccgaaacgt tgcaggaggc cgttcgactc gtgacccgac gagcaagcga
     9061 cttcagctcg ccgctcctgt cgctggagga gtatctggcg ctggggcgca agcgcgcccc
     9121 gaccttcgtc gcggatcgtc gtcggatcta tggcctcgcc ctccactacc aggaacggct
     9181 cggttcgggg aggttgtggg acgagatcga cctttgccgc gcggcgcttc agtccctgga
     9241 ggaatccggg caccaggggg cctacgacct ggtggtctgc gacgaggtcc aggacttcac
     9301 cgacgtgcag gtctccctgc tctttcgcct tgcgcgggat ccgcgttcgg tggtcctcac
     9361 cggagacccc cggcagatca tcaatcccag cggcttccgc tgggaagagg tgaagaacaa
     9421 gttctacgag cgggggctcc cggtaccgga cctctcgagg ctcagcctca acttccgctg
     9481 cgcgggaccc ctggttcgcc tggccaacgc cctgctcgag atgaaacagg ccttcgtcgg
     9541 caccgccgac acggatgccc gcgagcagtg gaagttcggc ggacgccccc cgctcctggt
     9601 gagccgggcc tccgaagagg agatcctggg cgagctcaag accgggagcg ccggccggat
     9661 cgttctcacc cgcacggagc cggagcggga ccggctgaag cgagccctct ccacggagct
     9721 cgtcttcacc atccaggacg ccaagggcct ggagttcgat acggtgctcc tctggaagtt
     9781 cgcctcggag gcagcctctc tgcccatctg gcgcggtatg caggcgggcc gaccggatcc
     9841 ggcccaggtg cccaggatcc gccacgagat cagccttctc tacgtcgccg tgacccgcgc
     9901 gcgaaactcc ctgatcctct acgacggcaa ggctccgtcc gacgtctgga ccagccccca
     9961 tctggtcgag cacctcttcc gcaccgagga cacggatcgc ctcgcggagc tgtggcgcgt
    10021 tctctctacg cctcaggagt gggagcggca gggagactac ttcttcgagc gacactacta
    10081 tcccgcggcg cgggagtgct tccgaaacgc cggaaccgtg gcaaaggcgg aggcggccga
    10141 ggggttcctg ctggcgcgcg ccggagagca cgcgcttgcc gcccctctcc ttgcgcgcaa
    10201 cggtcacgcc cggcaagcag ccgagagtta cgaggccgcc ggcatgccgc agcaggcggc
    10261 ggagctttgg gaccaggttg gagagacgcg ccgcgcacgg ctgtgccgga tccgcgcgct
    10321 ggaggcagcc ggtcagtggt cccaggcggc ggccgcctgg gagcaggagg gccagcgcga
    10381 tcgggccttt ccctgctggg agaaggccgg aaaccatcag tgggtgggtg aatacctcct
    10441 gtctgcgggc aagatcgaac cagccctgaa agcgctcgag aggtcgggag acgttgctcg
    10501 acaggcggag tgctggcgaa agctcgggaa gctcgagcgc gccgccgacc tgtacgcgcg
    10561 gaccggcgat gtggcccgtg cgatctccct gtaccgagag gtgggggcta ccgagaagct
    10621 cctgcgctgt ctccagcgca tcggagacta tcggggtgtt gccgtggagt acgagcgctc
    10681 cggggactgg gcaagggccg tcgatgcgta ccgtagactg gcggagtcct cggcgcctgg
    10741 ccgcaaggcg ctggaggacg agatctccga gcaaccccgg cagaggaaga cgatgatccg
    10801 gtcggcgctt cgcctgtcgg ccctcgggag ggaacgtgaa gcgggaccgc tgtaccgggc
    10861 ggccggcctg ttcgagccgg cggtccgggc tttccaggcc gtccaggact acgagcagct
    10921 tgcggaatgc ctttcccttc tgggtcggca ccgcgaggcg gcggccgccg ccgagaaatc
    10981 ggacctgccg gacgctcgac gggaagacct cgtcttccgt catctggaac gtcatctcca
    11041 ggccgacccg cggcgcgagt ctcgaaccgc tctggagctg gaggaggagg cggaccggct
    11101 gcttgccgaa ggcaagctgg tgcaagcgct ggtgcgctac cgagcgcttg gaaacggacc
    11161 gggggctctg gaagcctacg tccggctggg acgggacgag gaggcgatcc tggagttcct
    11221 ggcaggcggc tcgccggccc tggcgcgcaa gtacgtcgaa gccaaggaga tcaacgtctc
    11281 cgccgagttc ctgaggacgg tagccatgcg gccggaccct gaggagggga cgcgccaggc
    11341 cgcagcgccc gcggagcggt caccctcgga aaggcggcag atgctcaaag ccctgttccg
    11401 aaaagctctg cgcagactac ctcaggccga agccgaggat cttctggacg agtgctccga
    11461 ggcggtcttc gggttcccga tcctgcacga ggcttcgggg gcggattggg actggctcct
    11521 gtccatgaag cagtacaaca ccatcgtcca gatggtcgca tacattccgg cgacgcgccg
    11581 tgggccgagc gaagcggtac ggccctttgt gtcgcggctg gaaaccgagg ccatgcggac
    11641 cggcgacccg gcgcttctga cctgtcacgc cctggctacg gatccggatc gggtggcaga
    11701 ggcggcctcc ggcgtgagcc ttggggcgcg tacggtcgaa gtgcttggtc gctgccgaac
    11761 gcgataccgg gaggccgtgg agcttcagct cgggcgaaac aacatcgacg ccgcaatcga
    11821 ctactgtcgg ctcgccgaag actatgggct ggccggctcc ctgcacgaga gaatgggtga
    11881 gatgcgggag gccatcaact ccttcctctt ggccaaggac tacgccgcgg cgatgcgctg
    11941 tgcgcgggcc tcgggaaagg agccgctggt ggcgcgcgtg tacgagcgcc agggggatct
    12001 gaaagcggcc gtggcggtct ggcgaaggct tggccggccc cgcgaggtgg agcgggtgct
    12061 ccggaggctc gagaagtcgg gcggcaggaa caaggagcct tgatcggatg accgtgacgg
    12121 ccagctacgg cgtgatcgtg ctcgggaccg tcctgtggga tgccggggcc atcctgcaga
    12181 aacaggcgat cgaaaagctc ggttccgggc gccttcgggt ggtccggctc ctggggtccc
    12241 ctcggtggat gctcgggctc gcagccaacg cggtcgggtg gggtctgttc gtgcttggcc
    12301 tcgattcggt cccggtgtcc gtggcgcgca cggtcacggg aggcagctac gtggtcctgg
    12361 cgctgttctc cacggtcttc ttgcgcacgc ccttgaagct gccggagtgg ttggcactga
    12421 tctgcgtcac cgccgggatc gtgatcctgg gcgcgcaggg gcaggccggg cgcgtgacgg
    12481 cggacacgct ccttccgtcc gtgatccgcc tcgggctcgg gttgggagcg atcatcgttg
    12541 tctgcggatc gacattgctg gccactcgtg gggccggctc catcatcgct tttgccgcga
    12601 cctcggggct tctttccggc gccggggacc tgatggtgaa gctcatcacg attctggcgc
    12661 gcgaagaggc gcgcccgctg gctggcgttg gggtggcgct ggcggcgttg tccggggtcg
    12721 ggatggtcct tttctacgtc accggtatct acatgctctc ccgggcgtac caggtaggga
    12781 cggtggttgc cggcatggtc atctccgatt tctttgcccg cgtcgcggcg atcttcctgg
    12841 gcgcggtttg cctctccgag cctcttgtcg cggcgggaag agctggacct ctgtacgccg
    12901 tgggcttcct gatggtattg ggaggatccc ttcttctggg ccggttctct tcacgcccgg
    12961 tccgaatcgc ctcgagttga cacctccggt gatctcgtct aaaccggatt ccgtgaaaac
    13021 cttcgaggtt tgggcgatgg gagagctcct ggtggagatc atgcgccccc ggcgcgggat
    13081 gtccctctcg gaggccggtg attttctggg tccgttcccc agcggttcca ccggtatctt
    13141 catcgatacc atagcgcgcc tcgggcactc ggcggccatg atcagcggcg taggagacga
    13201 cgatttcggt cggtgcatcc tggagcggtt ccggcgggac ggggttgatt gcagccggat
    13261 cctggtggtg cccaaccagg cgaccggggt tgcgtttgtc acgtacttcc ccgacggctc
    13321 tcgaaggttt ctctaccact gggacaacac gccggccgtc atggccagga ggccggatcc
    13381 ggacctcatt caagagcctc ggtttctcca cgtgatggga tgcgccctca tgccgaacga
    13441 cctctttcgc gctgaggtct tcgagacggt caagctcttc gcatccaagg gcgcccgggt
    13501 aagcttcgac ccgaacatcc ggttcgagct cctgggcgga cgcaccgcgg agagcatcat
    13561 cggaccggtc atcgagcaca gttccgtgct gctgccgggg gagcgggagc tgcagctcct
    13621 ctccggaatc gaggacatcg acgccgccgc gcgccggctt ctctctcggc cgcgcatgga
    13681 gatcatcgtg gtcaagcgcg gaagtcgggg atgcaccgtg tactcgcgtg acggcaggat
    13741 cgacgtgccg ccataccggg ttcaggaggt ggacccaacg ggcgccggag actgtttcga
    13801 tgccggtttt atctgtggcc ttctggaagg gaagtccctt gaggaaagcg cccgcgtggc
    13861 ggctgctgcc ggggctctga acgcggccgc ctttggccct atggaagggg acatctcccc
    13921 gcggagcatc gccgcggtgc aggcccgctg aggtgaagac cgcgatgtct ctcacgagtg
    13981 gcgaccggcc cgtgtccccc ctcgtggaga tcgtcgcctc ccagaaacgc ggggaggcgc
    14041 ggggactcgg atccgtgtgc agcagcctcc gtcccgtgct ggaagccgct gtccagcagg
    14101 cactggagga cggcgcgccg ctcctggtcg aatccacgtg caaccaggtc aatcagttcg
    14161 gcgggtactc cggtctcaca ccggtggcct tcgtggccct ggtccgatcc ctggcgaacg
    14221 cttcgggtct gcccggcgcc cgactcgtcc tgggcggaga tcacctcggg ccgtatccgt
    14281 tccgcggaga gccggcccag agcgccatgg cgaaggcggc cgagatggtg cgggcctaca
    14341 tcgctgccgg ataccgcaag ctgcatctgg acgccagtat gcggcttgcc gacgatccgg
    14401 gggagccgcg aggtcccctg gacgtgcagc ggatcgccga gcggtgcgcg gacctctgct
    14461 cggcggcgga agcagcctgg gcggaggctc ggtccgaagg cgggaccgac gccgaagagc
    14521 cgccggtcta tgtgatcggc accgatgtgc ccactcccgg tggaagcaac gaggtggaat
    14581 ccgcggtcca cgtcaccacg gcctcagagc tccaggaaac gatcgagacg acccgacgac
    14641 ggttcctggc tggggggctc gtttcggcct gggagcgcgt cattgccgtg gtggtgcagc
    14701 cgggcgtgga gttcggcgat caggcgatcg tcgcctatga ccgccgcagg tcgggcgggt
    14761 tgatcgaggc cgtgcgcaag acaccgaggc tcgtgatgga gggtcactcc acggactacc
    14821 agacaccgcg agcgctgaga gagttggtcg aagatggagt ggcgatcctg aaggtgggac
    14881 cggcgctgag cgcggcggct cgcgaggcag tgttcctcct ggcccacgtg gaggaagaga
    14941 tatgtcggct acgcgcgggg ctcgaaccat cggggattgg ggacgccctc gaggaggcca
    15001 tgatcgccaa tcccgtccac tggcagggac actacacggg ggatgagaac cagcgttcct
    15061 tcgccaggag gttcagcctc ctggaccggg tccgctacta ctggggagtc cccgaggtag
    15121 agcgagccat ggcccggctc ttctcgaacc tccgcgcggc gccgatccct ctcccgctcc
    15181 tgagccagta tctgccccag cagcacggcc gaatccgtgc gggtgccctg accaacgatc
    15241 ccggcgcgat gctccgggat cggatccgcg aggtgctccg gggctactcg tgggccgcgg
    15301 gcttccagcc tggaccgaag gagggtgcgt gagctgccgt cctgggctcc ctgaattaac
    15361 gtaatcctaa cagaaccttc accctcgaat aacatcgccc tcctagattc gccatgatcg
    15421 attcgcaaac ctatcgttga aggaggattc ccatgaaggg tctacggatc agcctcgcgg
    15481 cgctcctgct gctgcttgcg gcaggcttcg gattcagcca ggcgattgag ctcgtcgg
//
LOCUS       DASRZS010000297         4937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2860101, whole genome shotgun sequence.
ACCESSION   DASRZS010000297 DASRZS010000000
VERSION     DASRZS010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2860101"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VFH83_07345"
     CDS             <1..999
                     /locus_tag="VFH83_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET6486218.1"
                     /translation="IAWDRLSGVIDADQRFVCAGASLRSRVLGALPGLSDGRFLGEPQ
                     GRDTLAAMALSAAVIGRNDPDAVLGVFTSDQIIEPVDAFRDVVVRGYDLAESMPDALV
                     TFGVKPVEPSPSYGYLELGDAVGGAAGKRSGARIVRRFREKPDRGTARSYLEAGPERY
                     LWNSGMFVWRASRFLELTRRYQPDVADAVERIRDSWDGPRREAVLREGYPGVRRISVD
                     FAVMEPASVDPSARVLAVPMDLSWRDIGSWPSYAEICRRDADGNAYAAETALLQESHG
                     TFVFSSDPSHLVAGVGLEDLMVVHTPRVTLVCRRDRAEEIKKLVEKLGKELGPGYQ"
     gene            complement(1102..1944)
                     /locus_tag="VFH83_07350"
     CDS             complement(1102..1944)
                     /locus_tag="VFH83_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6486219.1"
                     /translation="MVVFVTGATGYIGSAVVRELLKTGHTVVGLSRSARGAEALREAG
                     AEVRRGDLADLDGLRIGAAGADGVIHLAFDHEGAYADFAAALRTDLLAIQAMGGAIEG
                     SGKPFVVTNHMNGAASEKAALELNGVRAIAVSLSPSVHGEGDKHGFVPRLIGIARAKG
                     FSAYVGDGSNRWPAIHRLDAANLYRLALEKAPNGSRLSGRGDEGVPFRDIAELIGRKL
                     NVPARSIPREEAEAHFGFLGRVAATDIPAFHGSSEETRTLLSWRPVHPGLIADMEEGH
                     YFRS"
     gene            2036..2443
                     /locus_tag="VFH83_07355"
     CDS             2036..2443
                     /locus_tag="VFH83_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6486220.1"
                     /translation="MKVQELAEKSGLTAYTIRFYEKEGLLDGRHVRRDANNYRNYAAE
                     AMERLGLIKKFQSIGCSIAELKEVLHDKDSQTRTNREIIAWIRQKIREVEGKKNEYDQ
                     ILATLNRMLEYRTGVGRNRRQRRTSSSHAGKAP"
     gene            complement(2468..3517)
                     /gene="nadA"
                     /locus_tag="VFH83_07360"
     CDS             complement(2468..3517)
                     /gene="nadA"
                     /locus_tag="VFH83_07360"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HET6486221.1"
                     /translation="MPQATITAQGYYERMKARLSQVVPDFELKYKAELAWKIDQLKRE
                     RNAVILGHNYMEPALYHSIPDFTGDSLELCRKAAGTDADIIVFCGVRFMAETAKILNP
                     TKTVLIPSPRAGCSLAEAITAKDVRDLKARFPGVPVVTYVNTYADVKAESDYCCTSGN
                     AAKVVASLKTDRVIFLPDEYLARNVANETGKRIIFPTRTPRSSADADLDYQMIGWTGR
                     CEVHEKFTVEDIANIRKQFPGVVVLAHPECSPEVVAASDFSGSTAAMIRYVESVAAPR
                     YLLLTECSMGDNVAAANPNREMLRLCSVRCPHMNEITLEQTLASLEQTRYVVDVPEET
                     RVRALRAVERMLSIG"
     gene            3707..4522
                     /locus_tag="VFH83_07365"
     CDS             3707..4522
                     /locus_tag="VFH83_07365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6486222.1"
                     /translation="MPALLRYTLAVLLLAAAAVVDWATGAELSVQFFYLLSIGLMAWF
                     DSFPAAAVLAVISTGVVLFLNLHDLEPVSRGSPLPYWNALIAAATFLTFALVTSALSR
                     AQRRLKELARLDPLTGIANRRSFMEKATAELVRSRRSGRPFSLMYVDVDNFKRVNDTL
                     GHSAGDTLLRTVSSELCIATRETDVVARLGGDEFAVLLPETEPAGGKDAAEKIRGRLL
                     ASATRGGWPVTFSVGLVCCDRAPRSLAALVDTADRLMYSIKNSGKDGLAAQVL"
     gene            complement(4594..>4937)
                     /locus_tag="VFH83_07370"
     CDS             complement(4594..>4937)
                     /locus_tag="VFH83_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6486223.1"
                     /translation="YTNVPADQAQKNLQTLARLAQIFKKYNQYKISIEGHAVMVNWDN
                     PEAGKKEQDSVLIPLSKARAEAVKTALARQGIDASRMTTVGLGGSVPIVPFSDLDNRW
                     KDRRVEFVLVR"
BASE COUNT          827 a         1631 c         1631 g          848 t
ORIGIN      
        1 atagcctggg accggctctc gggtgtgatc gacgcggatc agcggttcgt ctgcgccggc
       61 gcgtctctcc gatcccgcgt gctgggtgcg cttccgggcc tgagcgacgg acggttcctg
      121 ggcgaacccc agggacggga cacgctggca gccatggcgt tgtccgccgc cgtcatcggg
      181 cggaatgatc cggatgcggt cctgggagtc ttcacctcgg accagatcat cgagccggtc
      241 gatgcctttc gagacgtcgt ggtccgaggc tacgatctgg cggagagcat gccggatgcg
      301 ctcgtcacct tcggggtcaa gcctgtcgag ccctccccga gctacggcta cctggagctg
      361 ggggacgcgg tgggcggcgc ggccggcaag cgttccgggg cccggatcgt tcgtcggttc
      421 cgagagaagc cggaccgcgg aacggcccgg tcctacctgg aggccggccc cgagcggtac
      481 ctgtggaaca gcggcatgtt cgtctggaga gcctcgcgct tcctcgagct gacgcgccgt
      541 taccagccgg acgtcgctga cgcggtggag cgcatccgcg actcctggga tggtccgcga
      601 agggaagcgg tcctgcggga ggggtacccc ggtgtgcgcc ggatcagcgt ggacttcgcc
      661 gtcatggagc cggcgtccgt ggacccgagc gctcgggtgc tcgccgttcc aatggacctg
      721 agctggcggg acatcgggag ctggccatcg tacgcggaga tctgccggcg cgacgcggac
      781 gggaacgcct acgccgctga gacggccctt ctgcaggaga gccacggtac gttcgtcttc
      841 tccagcgatc cgagccacct ggtggctggt gtcgggctcg aggatctcat ggtggtccac
      901 acgccgcgcg tcacgctggt ctgccggcgg gatcgagcgg aagagatcaa gaaactggtc
      961 gaaaagctcg ggaaagagct cggaccgggg taccagtagc cacccagccg cgagcaacgc
     1021 gccaacggcc tcggggtagt cccccggcca tccacaggcg gcccgtgcgc ggggccgccc
     1081 aaccgatcat gatcgggaga ctcacgatct gaaatagtga ccctcttcca tgtcagcgat
     1141 aagtcccgga tgcaccggcc gccaactcag gagcgtacgg gtctcctcac tcgagccgtg
     1201 aaacgcaggg atgtcggtgg ccgcgactct gccaagaaag ccgaaatgag cctccgcctc
     1261 ctcgcgggga atgcttcggg ccggcacgtt gagcttccgg ccgataagct cggcgatgtc
     1321 ccgaaagggc acaccctcgt cgccccgacc gctcaaccgc gagccgtttg gcgctttctc
     1381 cagcgcgagg cggtagaggt tggcggcatc gagccggtgg attgccggcc agcggttgga
     1441 gccatctccc acgtaggccg aaaagccctt cgcgcgggca atgccgatca gcctcggcac
     1501 gaaaccgtgc ttgtcccctt caccgtgcac ggacggggag aggctgacgg ctatcgccct
     1561 gaccccgttg agctccagcg ccgccttctc ggatgccgcc ccgttcatgt gattggtgac
     1621 cacgaaaggc ttgcctgagc cttcgatcgc gccgcccatg gcctggatgg ccaggagatc
     1681 ggtcctgagc gcggcggcga aatccgcgta ggcaccctca tggtcgaatg ccaggtggat
     1741 gacgccgtcc gctcctgctg ccccgattcg aagcccgtcc aggtcggcaa ggtctccgcg
     1801 ccgcacctcc gcgccggctt ccctcagcgc ctcagcgcct cgggctgaac ggctgagtcc
     1861 cacgaccgtg tggccggttt tgagaagctc ccggacgacg gcggagccga tatatcccgt
     1921 tgcgccggtt acgaacacta ccatgctgaa atcctcctga agccgctgcg gttaaaaccc
     1981 ttgcagaagg taaaccttag agtaaactcc aaggcaaggg atttccggga gtgctatgaa
     2041 ggtccaggag ctggccgaaa agtcgggatt gaccgcctac accattcgct tctatgagaa
     2101 ggaaggcctc ctggacggcc gacatgtccg cagggatgcc aacaactacc gcaactatgc
     2161 cgcggaggcc atggaacggc tggggctgat caagaagttt cagagcatcg gctgttccat
     2221 cgccgagctc aaggaagtcc tccacgacaa ggattctcag acgcgcacca atcgagagat
     2281 catcgcatgg attcgtcaga agatacgtga ggtcgaaggc aagaagaacg aatacgacca
     2341 gattcttgcg accctcaacc ggatgctgga atacagaacg ggggtgggtc gcaatcgccg
     2401 gcagagacga acgtcctcaa gtcatgcggg gaaagcgccg tgaccggaac cacgccaatc
     2461 tggcgtctca cccgatggag agcatcctct ccacggcgcg aagcgcgcgc acccgggtct
     2521 cctccggcac atccaccacg tagcgggtct gctcgagcga cgccagcgtc tgctccaggg
     2581 tgatctcgtt catgtgcggg catcggacgc tgcacagccg aagcatctcc cggttcggat
     2641 tggcggccgc cacgttgtcc cccatgctgc actccgtgag caggaggtag cggggcgcgg
     2701 ccacgctctc cacgtagcgg atcatggccg cggtactgcc ggagaagtcg gaggcggcca
     2761 ccacctcggg gctgcactcc ggatgcgcca ggaccacgac gcccgggaac tgctttcgga
     2821 tgttggcgat gtcctccacg gtgaacttct cgtgcacctc gcagcggccc gtccacccga
     2881 tcatctggta gtccaggtcc gcgtccgccg aggagcgcgg cgtacgcgtc gggaaaatga
     2941 tgcgcttgcc ggtctcgttg gcgacgttgc gggccaggta ctcgtccggg agaaagatca
     3001 cccgatcggt cttcagggac gccaccacct tggccgcgtt gccggaggtg cagcagtagt
     3061 cgctctccgc tttcacgtcc gcgtaggtat tcacgtaggt cacgacgggc accccgggga
     3121 accgggcctt caggtcccga acgtccttgg ccgtgatggc ctccgccagg gagcaacccg
     3181 ctctgggaga ggggatcagc acggtcttcg tgggattcag gatcttggcg gtctccgcca
     3241 tgaatcgcac gccgcaaaag acgatgatgt cggcatccgt gccggccgcc ttgcggcaga
     3301 gctccaggga gtcacccgtg aagtcgggga tggagtgata cagcgcgggc tccatgtagt
     3361 tgtgccccag gatgacggca ttccgctccc gcttgagctg gtcgatcttc caggccagct
     3421 cggccttgta cttcagctcg aagtcgggga ctacctggct cagccgagcc ttcatgcgct
     3481 cgtagtagcc ctgggcggtg atggttgcct gcggcatgtt ggtgctcctg tctcaacggt
     3541 acctacccgc cgagcctcgg gtcaaggacg aggcagaggc ccgagtccgc cgctccgcca
     3601 ctccgccgcc ccgcccttgc gcggcccgcc gggcccgccg ccacgccctt gcgccggact
     3661 ccgggcggtg ttagattcta cggaaatgtc gcagcgacaa ccacaaatgc cggcgctcct
     3721 ccgatacacg ctggcagtcc tgctcctggc ggccgccgcc gtggtggact gggcaacggg
     3781 ggccgagctc tccgtacagt tcttctacct gctctcgatc gggctgatgg catggttcga
     3841 ctcgtttccc gcggcagccg tcctggcggt gatctcgacg ggcgtggtgc tcttcctcaa
     3901 cctgcacgac ctggagcccg tctcccgggg aagcccgctc ccctactgga acgcgctgat
     3961 cgcggcagcc accttcctga ccttcgctct ggtcacctca gcgctgagcc gcgcgcagcg
     4021 ccgcctgaag gagctggcgc gcctggaccc ccttacgggg atcgccaatc gccgaagctt
     4081 catggagaag gccaccgctg agctggtccg ctcccgacgg tcggggcggc ccttctccct
     4141 gatgtacgtc gacgtggaca acttcaagcg cgtgaacgac acgctgggac acagcgcggg
     4201 ggacacgctc cttcggaccg tgagcagcga gctgtgcatc gccacacgag agacggacgt
     4261 ggtggcgcgt ctgggcgggg acgagttcgc cgtccttctg ccggagaccg agccggccgg
     4321 cggcaaggac gcggcggaga agatccgcgg tcggcttctg gcaagcgcca cccgtggggg
     4381 ctggcccgtg acgttcagcg tgggactggt ctgctgcgac cgggcgccga gaagcctggc
     4441 cgcgctcgtc gatacagcgg accgactgat gtactcgatc aagaactccg gaaaggacgg
     4501 tctggcggcc caggtgctct aggccgggcc gcgtaggccg ggccgcgtag gccgcgccgc
     4561 gcaggcctcc agccgtccgt ctccggaagg gaatcaccgc accaggacga actccacgcg
     4621 acggtccttc caccggttgt ccaggtcact gaacggcaca ataggcaccg agccgcccag
     4681 accgaccgtg gtcatccggc ttgcgtcgat gccttgtctg gccagggcgg tcttgaccgc
     4741 ctccgcgcgg gccttggaca gcgggatcag gaccgagtcc tgctccttct tccccgcctc
     4801 cggattgtcc cagttcacca tgaccgcgtg accctcgatg ctgatcttgt actggttgta
     4861 cttcttgaag atctgggcca gacgggccag ggtctgcagg ttcttctgcg cctggtccgc
     4921 gggcacgttc gtgtagt
//
LOCUS       DASRZS010000298         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_554774, whole genome shotgun sequence.
ACCESSION   DASRZS010000298 DASRZS010000000
VERSION     DASRZS010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554774"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1857)
                     /locus_tag="VFH83_07375"
     CDS             complement(166..1857)
                     /locus_tag="VFH83_07375"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HET6486224.1"
                     /translation="MSDPVTGRSASGSGEVREPQRDGTLRRGTDPARRRRIERAVVTA
                     LVIADLALALLVSGRYFFRADLSRNAAYTLSAASKSVLAALPERVTITYYVSDLLRNR
                     YPFPSEVEDLLAEYAGWSGGRISITSEDPSKSRRPLNLAALGVSGQQMQVVQRDQVNL
                     ATVYSGIVLQYLDRSSTIGFVSDLSTLEYDLTSRIRALVANRKDTLGLLVGDARKSLS
                     QDYGLLARSLAAQYQIQEVQRGGSIPPEVSVLFVLGTRDIDPPSMLAIDQYLMGGGKA
                     LFAVDPVDVDLGGGLAATVNKNQAVESALAAYGVVVRPQLVLDVLNQRLSFSTGQGSY
                     RIINYPHWITISGKDVDQTQPVTARFAGLDLYWPSPLELQSRPGVRGLALVRTSADAW
                     VQTARFSTNPMLPDTLQAPDSVDRAQYTVAAILSGTLPSAFAGQPLPTRPGEKPAPGP
                     RFLASSAPTRLIVVGNAGFASNMIQYTQAEYNLTFLSNCADWLLQQEDLLGIKTRAQV
                     DTRLDAIRDPLAKANVMRTAIVINVGLVPALIAAYGFVRLILRRRAKHLQPGRPA"
     gene            complement(1857..>2292)
                     /locus_tag="VFH83_07380"
     CDS             complement(1857..>2292)
                     /locus_tag="VFH83_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486225.1"
                     /translation="VVGKFLAVLFLLVLVTVLSLPIPLSLARLGAFDAGQIVAQYVGV
                     LLIGAAGIAVGLFISSLATNQVTAFVLGALALVAITLMGQLPRALSLSGWLADVLAYL
                     ALSQHYDSFRKGLLDSRDVLYFALICALFLFLNLRVLLRRKS"
BASE COUNT          447 a          742 c          774 g          329 t
ORIGIN      
        1 cgctcagcgt cacccgggca acggcatccg tctggagccc ggagaacagc gtccgttcgg
       61 atgcgcggcg agacgtcgcc aacggcgagc ggaacgcgcc gatcacgaaa gcagcgagca
      121 ggacgacgaa aaggccgccg agcgccagaa cgcgaatgcg gaagctcatg cgggcctccc
      181 cggctgcagg tgcttggccc gtctgcgcag gatgaggcgg acgaagccgt atgccgcgat
      241 cagcgccggc accagcccca cattgatcac gatagctgtc cgcatgacgt tggccttggc
      301 caggggatcc cggatcgcat ccagccgggt gtctacctgc gcccgggtct tgatgcccag
      361 gagatcctcc tgctgaagga gccagtccgc gcagttcgaa aggaacgtca ggttgtattc
      421 cgcctgcgtg tactgaatca tgttggaagc gaagcccgcg ttacccacga cgatcaaccg
      481 tgtcggcgcc gaggaggcga gaaagcgagg gccaggggcc ggcttctccc ccggacgggt
      541 cggcaggggc tgacccgcga acgcgctcgg gagcgttccg ctgaggatgg ctgccaccgt
      601 gtactgcgcg cggtccacgg aatccggcgc ctggagggtg tcggggagca tcgggttggt
      661 ggagaaccgg gcggtctgaa cccaggcgtc cgcggaggtc cggaccagcg caaggccccg
      721 gacgccaggg cggctctgga gctcgagcgg actgggccag tacagatcca acccggcgaa
      781 gcgcgcggtt accggctggg tctgatcgac atccttcccg gaaatcgtga tccagtgagg
      841 atagttgatg atccggtagc tcccctgccc cgtgctgaag gagagtctct ggttcagcac
      901 gtccaggacg agctgcgggc gcaccacgac gccgtaggca gccagcgcgc tttccacggc
      961 ctgattcttg ttcaccgtgg ccgccagccc accgccaagg tccacatcca cggggtcgac
     1021 cgcaaacagc gctttgccgc cgcccatcag gtactggtcg atggcgagca tggaaggggg
     1081 gtcgatgtca cgggtgccga gaacgaagag gacggagacc tccgggggaa tcgaacctcc
     1141 ccgctgcacc tcctggatct ggtactgcgc ggcaagggac cgcgccagaa gaccgtagtc
     1201 ctgcgacagg ctcttgcgcg cgtctcccac caggagcccg agggtatcct tgcgattggc
     1261 caccagggcc cggatccggg atgtgaggtc gtactccagc gtgctcaggt ccgacacgaa
     1321 gccgatggtg gagcttcgat ccaggtactg caggacgatg cccgaataga ccgttgccag
     1381 gttgacctgg tcccgctgga ccacctgcat ctgttggccg ctcaccccga gagccgccag
     1441 gttcagcggg cgcctggact tcgacgggtc ttcggacgtg atcgagatcc ggccgccgga
     1501 ccaaccggcg tattccgcca ggagatcctc cacctcggac ggaaatgggt accggttccg
     1561 cagcaagtcg gacacataat acgtgatcgt gacccgctcc gggagcgcgg caagcaccga
     1621 cttcgaggcc gcggagagcg tataggccgc gttgcggctc agatctgccc gaaagaaata
     1681 ccggccgctc accaggaggg ccaacgccag gtccgcgatc accagagcgg tgacaaccgc
     1741 ccgctcgatc cggcgccttc gggccgggtc cgtgccgcga cgcagggtcc cgtccctttg
     1801 cggttcgcgg acctcaccgc ttcccgaggc ggaccgcccg gtgacgggat cgctcatcac
     1861 gatttcctcc gcaagagcac gcgaaggttc agaaacagga acagggcgca gatgagcgca
     1921 aagtagagga cgtcccggct atcgagcaac cccttgcgga aactgtcgta atgctgggaa
     1981 agggccaggt aagccaggac gtccgccagc caaccgctca acgacagggc tcggggaagc
     2041 tgccccatca gggtgatggc cacaagggcc agggcgccca ggacgaatgc cgtgacctgg
     2101 ttcgtggcca gggaggagat gaagagaccg accgcgatgc cggccgcgcc gatcaggagc
     2161 accccgacgt actgggcaac gatctgtccc gcgtcgaacg ctcccagcct cgccaggctc
     2221 aacgggatgg gcagcgagag caccgtcacc agcacgagga ggaaaagcac ggcaaggaac
     2281 ttgccgacga cc
//
LOCUS       DASRZS010000299         4695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1323221, whole genome shotgun sequence.
ACCESSION   DASRZS010000299 DASRZS010000000
VERSION     DASRZS010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323221"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFH83_07385"
     CDS             <1..606
                     /locus_tag="VFH83_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486226.1"
                     /translation="IVGVLLIGTSFGINNGPSASVTAAELAAFGAAHSGAIMVGAWLQ
                     AVGPVLIIFFAFALVHQAGLAGKLSGWMTLFGAVVLMMVSLTECVFYVAALFPLPESQ
                     ALIATDIAHAVQHFYFIVAAPALFLPLGIVVITSGVLPRVLGWLAVVLAAAFAVLGIT
                     SLHSLVLSAATTSLAAVQALWWLAAAIALMARKPRAAEGQQ"
     gene            603..1487
                     /locus_tag="VFH83_07390"
     CDS             603..1487
                     /locus_tag="VFH83_07390"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-polyphosphate phosphotransferase"
                     /protein_id="HET6486227.1"
                     /translation="MKIPTEQYRVAEHEKVDLGKRQTRVAPVCQSRKEYEQLLEDHVR
                     SLSSLQNLHYAADRCAVLLVFQAMDAAGKDGTIRHVLSGVNPQGCQVYSFKQPSAEEL
                     QHDFLWRAERCLPERGRIGVFNRSYYEDVLVVRVHPELLANAGIAASSAADEQLWKDR
                     YHSIRHFEKHLARSGTHIVKFYLHLSAEEQRQRFLQRIEDPERNWKFSEADIRERRFW
                     GDYMRAYEQCIQETSLEEAPWYIVPADDKETARLIVSQIVLDALEALKLSYPETPPAR
                     KRELESFRKALETETHPA"
     gene            1905..3038
                     /locus_tag="VFH83_07395"
     CDS             1905..3038
                     /locus_tag="VFH83_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6486228.1"
                     /translation="MVTIRDVAERAGVSTTTVSHALNGTRFVNPEKAARIFSAARELG
                     YTPNPAARVLRTGATRIIGALGPSSLDPFFAEAIDGIEDVCSSRGYDLLLGYAQYAVG
                     TIWGKTDVERSAEVEFLRAVMDGKSDALHPSGAQLASAPSREMSKVSRFLARGAKGLI
                     LNLYQPDDVVLKVLQGASAKIALFHRRIEGLEADRFGSDDYGGTMQAMEALLALGHRR
                     IGMIYAYSQPGHGVRARFLAYRDALQKRGIEVDLTLLLNGGHSPAVAATATETLLALA
                     DPPTAILYWGDGMALPGMDAARRAGRSIPQDLSIVGFDDLPFASLISPRLSTIRQEVV
                     GLGAVAAARLIDRIEGKIQGPAETVSLPMRFVARESTGPAPGR"
     gene            3124..3546
                     /locus_tag="VFH83_07400"
     CDS             3124..3546
                     /locus_tag="VFH83_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6486229.1"
                     /translation="MAMNPVVHFEMAARDGSRMAKFYAGVFGWKTNQLGAEMGNYITA
                     QTTESAESGLPKEPGRINGGFFTRTGEDQGTSVVIAVENIRDAMKRVAAAGGKVLGGQ
                     KPGEPDDIPGVGLYSAFIDTEGNRVGMLQPAPMPGSNR"
     gene            3746..>4695
                     /locus_tag="VFH83_07405"
     CDS             3746..>4695
                     /locus_tag="VFH83_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET6486230.1"
                     /translation="MMEGIQAIILDSITEGVFTIDDSFRITSFNRAAEKITGIPRERA
                     VGKPCMEILRASICEGRCALDRTMRTGQPAIGKTVFILDGEGSRKRISITTALLKDEG
                     GKIVGGVETFRDLSVIDSLRRELHKEYSFEDILSRSHHMRKLFDILPNVAESGTTVLL
                     EGESGTGKELFARAIHSLSPRSKKPFVAVNCAALPDTLLESELFGYRAGAFTDARKDT
                     PGRFARAEGGTLFFDEIGDISPAMQVRLLRFLQERTYEPLGASQPVSADVRVIAATNR
                     KLSDLVRGGVFREDLFYRINVVCLSLPPLRERMEDIPLLVG"
BASE COUNT          821 a         1473 c         1576 g          825 t
ORIGIN      
        1 atcgtgggcg tgctcctgat tggaacgtcc ttcggcatca acaacggacc ctcggcctcg
       61 gtcacggccg cggagctcgc ggcgttcggc gcggcgcact ccggggccat catggtgggg
      121 gcgtggctgc aggccgtagg gccggtcctc atcatcttct tcgcctttgc cctggtgcac
      181 caggccgggc tcgcggggaa gctctccgga tggatgacgc tctttggcgc cgtggtcctc
      241 atgatggtga gcctcacgga atgcgtcttc tatgtcgccg cgctcttccc gctgccggag
      301 tcgcaggcgt tgatcgccac cgacatcgcc cacgcggtgc agcacttcta cttcatcgtc
      361 gccgcgcccg cgcttttcct gccgctgggg atcgtggtaa tcacttccgg agtccttccc
      421 cgggtcctgg ggtggctggc cgtggtcctc gcggccgcct tcgcggtgct ggggatcacg
      481 tctctgcaca gcctggtgtt gtcggccgcg acgacgtccc tggccgcggt gcaggcgctg
      541 tggtggcttg ccgcagcgat cgcgctgatg gccagaaagc cgcgcgcggc tgaggggcaa
      601 caatgaagat accgacggag cagtaccggg tggccgagca cgaaaaggtg gatcttggca
      661 agcggcagac ccgcgtcgcg ccggtgtgcc agtcccgcaa agagtacgag cagctcctgg
      721 aggaccacgt tcgatctctg agctcgctcc agaacctgca ttacgcagcc gatcgctgcg
      781 cggtgctcct ggtcttccag gccatggatg ccgccggaaa ggacggcacg atccgccacg
      841 tgctgtccgg cgtgaatccg cagggctgcc aggtgtactc cttcaagcag cccagcgccg
      901 aggagctgca gcacgacttc ctctggcggg ccgagcggtg ccttcccgag cggggacgca
      961 tcggggtctt caaccgctcc tactacgagg acgtgctggt ggtgcgtgtg cacccggagc
     1021 ttctggcgaa tgcgggcatc gcggcgtcct ccgccgccga cgagcagctc tggaaggacc
     1081 gctaccactc gatccgccac ttcgaaaagc acctggcccg cagcgggacc cacatcgtca
     1141 agttctatct ccacctctct gccgaggagc agcgccagag gttcctccag cgcatcgagg
     1201 atcccgagag gaactggaag ttcagcgagg ccgacatccg cgagagacgg ttctggggcg
     1261 actatatgcg agcctatgag cagtgcatcc aggaaaccag cttggaagag gctccctggt
     1321 acatcgtgcc ggcagatgac aaggaaaccg cacggctgat cgtctcccag atcgtcctgg
     1381 acgcgctgga ggcgctcaag ctctcctatc ctgagacccc gcccgcgcgc aagcgggaac
     1441 tggagtcctt ccgaaaagcc ctggagaccg aaacacaccc tgcctgaggg gctctgagcg
     1501 cgccaagtgt tgggcgcttt ttcacttgac gaccgctctg ccatcgcagt agcttagcca
     1561 atcgattgcg caaacgattg gccaatcgat tgcctggtgc tagagaggag agccggtatg
     1621 ctggaaggtc aggggggtat gggtcagatg ctgctcggac agagcgatga catacacaga
     1681 cttgctgaaa ggaccgctga gccctggcgg ttcgccgatc tcgccgcgcc gtcgacgtcg
     1741 agaggtagaa gggttgaagg gctcagaggc ttgcgtggta ttctggcgtc cctggtggtc
     1801 ccgttggccc gctcgcggac tcggggcagc ccacagacca cctgctgttc ggagccctga
     1861 ggccggacgg attgtctgca agggagcgtg gagtcggagg tgccttggta accataaggg
     1921 acgtagcgga acgagcgggg gtctctacca ccacggtctc tcatgcgctg aacggcacgc
     1981 ggtttgtcaa cccggagaaa gccgccagga ttttctccgc cgcccgcgag ctgggctata
     2041 cgccgaaccc agccgcccgg gtgctgcgaa cgggggccac gcgcatcatc ggagccctgg
     2101 gaccaagctc actggatcct ttctttgcgg aagccatcga cgggatcgag gacgtgtgtt
     2161 cttcgagagg atacgacctg cttcttggat atgcccaata cgccgtcggc acaatctggg
     2221 gaaagaccga tgtcgaacgc tcggcggagg tcgagtttct ccgagccgtg atggacggca
     2281 agtcggatgc gctacacccc tcgggggcgc aactggcctc cgcgccttcc cgggagatgt
     2341 cgaaggtctc caggttcctg gctcggggtg ccaaagggct gatcctcaac ctctaccagc
     2401 cggatgatgt cgtactcaag gtccttcagg gcgcgtcggc caagatcgcg ttgttccatc
     2461 gccggatcga aggtctggaa gccgatcggt tcgggtcgga cgactacggc ggcaccatgc
     2521 aggccatgga ggcgttgctg gccctggggc accgcaggat cggcatgatt tacgcctact
     2581 ctcagccggg gcacggagtg cgcgcgcgat tcctggccta ccgggatgcg cttcagaagc
     2641 ggggcatcga ggtcgatctg acgctcctgc tgaacggcgg tcattcgccc gcagtagccg
     2701 ccacggcgac cgaaacgctt ctggcactcg ccgatccacc aacggcaatc ctctactggg
     2761 gcgacgggat ggcgctgccg ggaatggacg ccgcgcggcg ggcgggccga tccatccccc
     2821 aggacctttc gatcgtcggc ttcgacgatc tcccctttgc gagcctgatc tccccaaggc
     2881 tttccaccat tcgccaggag gtggtgggtc tgggcgcggt agccgctgca cggctcatcg
     2941 atcgtatcga agggaagatc caggggcccg cagaaaccgt gagcctgccc atgagattcg
     3001 tggctcggga gagcaccggc ccggcgcccg gccggtagcg acccacggcg gccaccgatg
     3061 cgagtgacgc cggtggccgg gcggacgcgc cagggttatc ttcgggagga agggagctca
     3121 gcgatggcga tgaatccggt ggttcatttc gagatggcgg cccgagatgg ctcgcggatg
     3181 gcgaagttct acgccggcgt gttcggttgg aagacgaacc agcttggtgc cgagatgggg
     3241 aactacatca cagcccagac gaccgaatcc gccgagagcg gactccccaa agagccggga
     3301 aggatcaacg gcggcttttt cacgcgtacg ggggaagacc agggcacgag cgtggtgatc
     3361 gcggtggaga acattcggga cgccatgaag cgcgtcgccg cggccggcgg caaggtcctg
     3421 ggcggccaga agccgggcga accggatgat atccccgggg tcgggttgta ctcggcgttc
     3481 atcgatacgg agggcaaccg tgtcggaatg ctgcagcccg ctcccatgcc gggctccaac
     3541 cggtaggact gcctccgctc gcccactgtg gaccgccgcc ccggtaacct ctagcctcgg
     3601 gcggtgtccg tccggggcgg agtcggagcg cggacggact gtgcccaggg acgcggggat
     3661 aacggccgcc ggtccccttg cggcttcccg gtcctgaggt gtacgctcac cgagcaatgc
     3721 ctcgcctgct cgagtggccc ccgcgatgat ggaaggcatc caagcgatca tcctggattc
     3781 catcacggaa ggggtcttca ccatcgatga ctccttccgc atcacctcct tcaaccgcgc
     3841 tgcggagaag atcactggaa tcccccgaga acgcgccgtg ggaaaaccat gcatggagat
     3901 cctccgcgcg agcatctgcg aaggtcggtg cgccctggac cggaccatgc ggacgggcca
     3961 gccggcgatc gggaagaccg tgttcatcct ggacggcgag ggcagccgga agaggatcag
     4021 catcacgacg gctctgctga aggacgaagg agggaagatc gtagggggcg tggagacctt
     4081 ccgggatctg agcgtgatcg atagcctccg gagggagctg cacaaggagt acagcttcga
     4141 ggacatcctg agccgcagtc accacatgcg caagctcttc gatatcctgc cgaacgtggc
     4201 ggaatcgggt accacggttc tgctggaagg ggagagcggg acgggcaagg agctgttcgc
     4261 cagggcgatc cactccctca gtccgcggag caagaagccc ttcgttgccg tcaattgcgc
     4321 cgcgctaccg gacaccctcc tggaatccga gctgttcgga taccgggcgg gcgcctttac
     4381 ggacgcccgg aaggacacgc cgggtcgctt cgccagggcg gagggcggga cgttgttttt
     4441 cgatgagata ggcgatatct ctccagcaat gcaggtgcgg ctgctgcggt tcctccagga
     4501 gcggacctac gagcccctgg gggcgtccca accggtatcc gcggacgttc gcgtcatcgc
     4561 cgccaccaac cgcaagctct cggatctcgt tcgcggcgga gtcttccgcg aggacctctt
     4621 ttaccggatc aatgtcgtct gtctctctct gcctccgttg cgcgagcgca tggaggacat
     4681 ccctctcctg gttgg
//
LOCUS       DASRZS010000300         7535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1323328, whole genome shotgun sequence.
ACCESSION   DASRZS010000300 DASRZS010000000
VERSION     DASRZS010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323328"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VFH83_07410"
     CDS             <1..230
                     /locus_tag="VFH83_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET6486231.1"
                     /translation="QLAMDALSDGDSPLTGRERDVLEAVGRGESIAATARRLFLSEGT
                     VRNYLSMAIQKVGAANRIEAWKTARDKGWLA"
     gene            318..698
                     /locus_tag="VFH83_07415"
     CDS             318..698
                     /locus_tag="VFH83_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET6486232.1"
                     /translation="MFKKIEAIIREQALGEVREALAAIGIVGMNVFEIRGRGRHGDQE
                     HSSIYTYRHGDMLPKIQLNIVLSERNVEPTVAAIMKAARTDTEGDGIIFVYPVEDVFR
                     VRTGERGGDALSYPDDIDARRSPD"
     gene            667..1161
                     /locus_tag="VFH83_07420"
     CDS             667..1161
                     /locus_tag="VFH83_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET6486233.1"
                     /translation="MTSMPAARRIDEVNGDSGVYLFALELGGSRSITIGALGRIRFEK
                     GWYLYVGSARRGLSARLARHERTGPGKAMKWHIDFLRAHARAVRSFPVPTRRDLECRL
                     ARDVQRLTAAPVPRFGSSDCACPSHLFRLAGDPRGDPRIAALLDRYRNRTASGDPVPQ
                     RQGG"
     gene            1164..1628
                     /locus_tag="VFH83_07425"
     CDS             1164..1628
                     /locus_tag="VFH83_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6486234.1"
                     /translation="MPRNHAWFVFLALVGAHLLFAGVSAIAESAPGAAGTGAGSQPSY
                     QDPDQLQVLITTRPEPYYLVDVRTPQEYNLGHVPTAMNIPYDVIAAQPPTSDKSALII
                     VYCESGGRSAKAKQSLDNLGYLRVIDFGGVSRWKGSSITGADAGDCPCRVHY"
     gene            1798..3264
                     /locus_tag="VFH83_07430"
     CDS             1798..3264
                     /locus_tag="VFH83_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET6486235.1"
                     /translation="METSASWNELRKHTTPQWFRDAKFGIYTHWGVYSVPARGPNATW
                     YPYNMYRENNEQYEYHKKTYGPPEKYGYREFISQFTAEKFDPDQWAELFRESGAQFAG
                     PVGEHHDGFCMWDTAYSEWNARKMGPKRDVVAELERAIRSQGLRFMVALHHAENWWFF
                     PHWRKEFDTTDPRFEGLYGKAHDVDMDPKKALRLRPHELWPIMEKPTKEFLDVWLGKT
                     REVIDRFRPDLLWFDFALDYVQEHYKRAMFSYYYSKAEEWKKEVVITYKWHHAVPGSA
                     VIDLELGRFSEMTYHDWITDTTVDDGSGWGYLKDTRYKSATSLIQYLVDNVSKNGYML
                     LNVGPKPNGEIPTEAVSILKAMGAWLRVNGEAVYGTTPWMLFGEGPTVMKKAGPFNED
                     QTLAYTPEDFRFTVKGDTLYAICLNVPRKSVTIKSLYQRLYPSEIVSVSILGTEERPP
                     WTFNPDGLTISVPSNLPLEHAVVFKIARKEPFPIGGGR"
     gene            complement(3311..4276)
                     /locus_tag="VFH83_07435"
     CDS             complement(3311..4276)
                     /locus_tag="VFH83_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6486236.1"
                     /translation="MGVRKEQFVSALEHRVPSRHVPLWELHFHLWNAFCGGGFVSGRE
                     YQKLSAAERDRALRDDARIMIDVADRLGFSGVTIPDAPWDCVYTLPPEDRLALAACLR
                     AESPDFLITAGCGGVMAIPDSPEYVEFCYRLVDAPDEVEEQCEARLQKGLESLRRYAD
                     CGIEAAYVAADMADNRGPFFSDAQMDRFILPYLARWAEGARKAGIYPILHTDGNITSL
                     LADLASTGICGIQALDPVAGMDIRRVKAAMERRLCLCGNVDCGALILSTPQRLYELTR
                     ELLLSCKPGGCFVLGASNAVVDTTPKENYEAVVRAWRDHGSYRSA"
     gene            complement(4421..6121)
                     /locus_tag="VFH83_07440"
     CDS             complement(4421..6121)
                     /locus_tag="VFH83_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486237.1"
                     /translation="MTRRIGWCVLIVAIIVVGPLFAGGQKEGASSSQAAVSAPGELPL
                     VKTPTTLTALIEPFGNITDITTNAYTKWMEQKTGVHLDFQVVPQTADSSSKIALILSS
                     GKLPDMIIGAAMPSGWQETYGGPKGVLLPLNDLIQQHGFYLKQVIQQDPAYLADSVLS
                     DGQIYMLGAWEECYHCSVAEKMWMNTDWLKKLNLKMPTTTAEFENVLKAFKAGDPNGD
                     GQADEIPLSGALNTWHSTLDGFLMNAFIYDDGQNRMNVDASGKVIPAFSQPQFREGLK
                     YMHQLYAEGLLDPQAFVQNLSQLQQLVMGPHVLVGAAMVGHRGMMTTNEYSPQSFAYE
                     VVPPLKGPNGVQFSGYFPYSPHTDGSDAAITRACKNPALAFKWLDYQFSQDASISNWN
                     GIQGVDWNPAKPGEIGRDGKPALFEYVNGPQNNPVAQSQQNRAWVHTGPYFMPAHWFG
                     GGAIEQGKFEYETFLYGASDPYVPFAPKIVMPNAMPFLPSDAKIVATMQQQMNDFVYS
                     AVAKFTTGQLDITSDSDWQQYLDQLNSMGLPDYTKAYQNGFSLWKKDVDAGLGMLTSK
                     "
     gene            complement(6241..7092)
                     /locus_tag="VFH83_07445"
     CDS             complement(6241..7092)
                     /locus_tag="VFH83_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020620647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6486238.1"
                     /translation="MLNGVFLGIVCVLVVYPLVYIVSNSFSDPNEVIAGSVWLYPVRP
                     SVSAYNETLTYASIWTGYINTIFYTITGTLVSVALTLLAAYPLSRTDFRGRSVYLFIV
                     FFPMIFSGGIIPTYLLVRSLGMVDTRWAMILPFAVSSWNVIITRTYYQYTISPSLLDA
                     ATIDGCSDLRFFSSVVLPLSGAITAVNVLFYAVGIWNQFFWPFLYLNRQQLFPLQLIL
                     QKILILNSMDTKGGSISVEAEDKLHLLYATMKYAVIIVASLPVLCVYPFVQKYFVRGV
                     MIGALKG"
     gene            complement(7158..>7535)
                     /locus_tag="VFH83_07450"
     CDS             complement(7158..>7535)
                     /locus_tag="VFH83_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014370468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6486239.1"
                     /translation="SIIYLAALASVDPELHEAATVDGASRFQRTVHVDFPGILPTVVI
                     VLILSLGQIMNLGFEKAYLMQNPLNLTSSEIIQTYVYKVGMSGVPQFSYAAAIGLFNS
                     VINLVLVLGANQVARRLGGVSLW"
BASE COUNT         1452 a         2335 c         2421 g         1327 t
ORIGIN      
        1 cccagcttgc catggatgcc ctcagtgatg gggatagccc cctgaccggg cgggagcgcg
       61 acgtcctgga ggccgtgggc cgcggggaga gcattgccgc caccgcccgg cggctcttcc
      121 tctcggaggg caccgtgcgc aactatctct ccatggcgat ccagaaagta ggagccgcga
      181 atcggatcga ggcgtggaaa accgcgcggg acaaagggtg gctcgcgtaa gcgcacgacg
      241 cccgcgcagc gcgcacgacc ctcgggcaga agccggcacg agggcgcatc ggcgcgcacg
      301 acgaaggagg acatccggtg ttcaagaaga tcgaggcgat catccgcgaa caggcgctcg
      361 gggaggtgcg ggaggcgctg gccgccattg ggatcgtcgg catgaacgtg ttcgagatcc
      421 gcggccgcgg ccgccacggg gaccaggagc actcgtccat ctacacctac cggcacggag
      481 acatgctgcc aaagatccag ctcaacatcg ttctcagcga gcggaacgtc gagccgaccg
      541 tggcggccat catgaaggcc gcccgcacgg acacggaggg agacggcatc atcttcgtct
      601 atcccgtcga ggacgtcttt cgggtacgga cgggggagcg gggaggagac gccctctcct
      661 atcccgatga catcgatgcc cgccgctcgc cggattgacg aggtgaacgg cgactccggc
      721 gtctatctgt tcgccctgga gctgggcggc agccgatcca tcaccatcgg ggcgctgggg
      781 cgcatacgct ttgagaaggg gtggtacctg tacgtgggct cggcccgacg cggactctcc
      841 gcgaggttgg cgcgtcacga gcgcacgggc ccgggaaagg cgatgaagtg gcacatcgac
      901 ttccttcgcg cccatgcccg tgcggtgcgc tcattccccg ttcccacacg ccgtgacctg
      961 gagtgccggc tggcgcgaga cgtgcagagg ctcaccgccg cgccggtgcc gcgctttggt
     1021 tcctcggact gcgcctgccc ttcgcacctc tttcgcctgg caggagaccc gcggggggat
     1081 ccgcgcatcg cggcgctgct ggaccgctac cgcaaccgga ccgcttcggg agacccggtg
     1141 ccgcaaaggc agggaggttg aaaatgccgc gaaaccatgc ctggttcgtg tttcttgccc
     1201 ttgtcggcgc tcacctgctc ttcgcgggcg tttcggccat cgcggagtcc gcccctgggg
     1261 ctgccgggac tggggctggg tctcagcctt cgtaccagga ccccgaccag ctacaggttc
     1321 ttatcactac caggccagag ccgtactacc tggtggatgt tcgcactccc caagagtaca
     1381 acctgggtca tgtcccaacg gcgatgaaca tcccgtacga cgtgatcgcc gcccagcccc
     1441 caacctcgga caagtcggcg ctcatcatcg tgtactgcga gtccggtgga cgctccgcca
     1501 aggcgaagca atccctggac aacctggggt acctgcgcgt catcgacttt ggcggcgtgt
     1561 cccgctggaa gggatcgtcc atcacgggcg ccgatgccgg tgactgcccg tgccgggtcc
     1621 attactagcg gcgtggcgct gaggcaacga gccgcgctga agcacacggc cgcactgagg
     1681 tacggcgacg cactggagca cgggtcgggg cggtcacctg cagaagggcc gtgttgacaa
     1741 gatcgcccac cagtattacc ctagttagta atctatattt acgaacggga gggccccatg
     1801 gaaacgtcgg cgtcctggaa cgaactgcga aagcacacca ctccccagtg gttcagagat
     1861 gcgaagttcg gcatctacac gcactggggc gtatacagcg tgcccgcgcg gggacccaac
     1921 gcgacgtggt acccgtacaa catgtaccgg gagaacaacg aacagtacga gtatcacaag
     1981 aagacgtacg gtcccccgga gaagtacggc taccgggagt tcatctccca gttcaccgcg
     2041 gagaagttcg atccggatca gtgggcggag ctgtttcggg agtccggagc gcagttcgcg
     2101 gggccggtcg gcgaacacca cgatggattc tgcatgtggg acaccgctta ctccgaatgg
     2161 aacgccagga agatgggccc caaacgggac gtggttgccg agctcgagcg ggccatccgc
     2221 tcccagggac tgcgcttcat ggtggccctg catcacgcgg agaactggtg gttcttcccg
     2281 cactggcgga aagagttcga caccacggat ccgaggttcg agggcttgta cggaaaggcg
     2341 catgacgtcg acatggatcc gaagaaggcc cttcgactgc gcccgcacga gctctggccg
     2401 atcatggaga agcccaccaa ggagtttctg gatgtgtggc tcgggaaaac ccgcgaggtc
     2461 attgaccggt tccgcccgga cctcctgtgg ttcgatttcg ccctcgacta cgttcaagag
     2521 cactacaagc gagcaatgtt ctcctactac tactccaagg ccgaggagtg gaagaaagag
     2581 gtggtgatca cctacaagtg gcaccacgcc gtccccggca gcgccgtgat cgatctggag
     2641 ctcggacggt tcagcgagat gacctaccac gactggatca cggacaccac ggtggacgac
     2701 ggttccggat gggggtatct gaaggacacc cggtacaaga gcgccacctc cctcatccag
     2761 tatcttgtcg acaacgtcag caagaacggg tacatgctcc tcaacgtggg gcccaaaccg
     2821 aacggtgaga ttccgacaga agcggtctcc atcctcaagg cgatgggcgc gtggctcagg
     2881 gtcaacgggg aggcggtcta cgggacgaca ccgtggatgc tctttggaga gggtccgacg
     2941 gtcatgaaga aggccggccc cttcaacgag gaccagacgc ttgcctacac cccggaggac
     3001 ttccggttca ccgtcaaggg cgacacgctg tacgcgatct gtctcaacgt gccgcgcaag
     3061 tccgtgacga tcaagagcct ctatcagcgg ttgtatccct cggagatcgt ctccgtgtcg
     3121 attctcggaa ccgaagagag gccgccgtgg acgttcaatc cggatgggct gacgatctcc
     3181 gtgccgtcga acctcccgct ggagcacgcc gtagtgttca agatcgcgcg gaaggaaccc
     3241 tttccgatcg gcggaggccg atgacggctg gggagacgcg gattccgcgc ccctccgagg
     3301 cggagacccc tcaagccgaa cgatagctgc cgtggtcccg ccaggcgcgc actacggcct
     3361 cgtagttctc cttgggagtg gtgtccacca ccgcgttgga tgcgccaagc acgaagcatc
     3421 cgccgggctt gcaggatagc agcagctcgc gggtgagctc gtagagccgc tgcggagtgg
     3481 agaggatcag cgctccgcag tcgacgtttc cgcacaggca gagccggcgt tccatcgcag
     3541 ccttgacccg ccggatgtcc atacccgcca ccgggtccag ggcctgaatg ccgcagatcc
     3601 cggtagaggc cagatcggcc agaagactcg tgatgtttcc atccgtgtgc aggatggggt
     3661 agatgccggc cttgcgggcg ccttcggccc accgcgcgag gtacggcaga atgaaacggt
     3721 ccatctgggc gtcgctgaag aacgggccgc gattgtccgc catgtcggcc gcgacgtacg
     3781 cggcttcgat gccgcagtcc gcgtatcgcc tcagcgactc gagccccttc tgaagccggg
     3841 cttcacactg ctcctcgact tcatccgggg cgtccaccag acggtagcag aactcgacgt
     3901 actcaggtga atcggggatg gccatgaccc cgccgcagcc cgcggtgatg aggaaatccg
     3961 gcgactcggc tcggaggcag gcggcgagcg ccagccgatc ctccggcggc aaggtgtaga
     4021 cgcaatccca cggcgcgtcc gggatggtca cgccgctgaa gcccaagcgg tccgcgacat
     4081 cgatcatgat gcgcgcatcg tcgcgcaaag cacggtcccg ttccgcggcg ctcagcttct
     4141 ggtactcccg accggagacg aaccccccgc cgcagaatgc attccaaagg tggaaatgca
     4201 gctcccaaag cgggacgtgg cgggaaggca ctcgatgctc gagcgccgac acgaactgct
     4261 ccttgcggac tcccacggat tcgctccttc cggacggcct tcgacggttg cactggggat
     4321 cttcgagagc gccccggcga atcgccaggc gactgccggg gccactctgt ccgttacgga
     4381 tcggtagcgc ccgatcccga cggatcgctt ctggatgacc ctacttgctc gtcagcatgc
     4441 ccaggcccgc atccacgtcc tttttccaca ggctgaaccc gttctggtag gccttggtgt
     4501 agtccggcag gcccatcgaa ttgagctggt ccaggtattg ctgccagtcc gagtcgctgg
     4561 tgatgtcgag ctgtcccgtc gtgaacttcg cgaccgcgga gtagacgaaa tcgttcatct
     4621 gctgctgcat cgtggcgacg atcttggcat cgctggggag gaacggcatg gcgttcggca
     4681 tgacgatctt cggggcaaac ggcacgtacg gatccgacgc cccatagagg aacgtctcgt
     4741 actcgaactt gccctgctcg atggcgccgc cgccgaacca gtgagcgggc atgaagtacg
     4801 gccccgtgtg cacccaggcc cgattctgct gggactgggc aacggggttg ttctgcggac
     4861 cgttcacgta ctcgaagagc gccggcttcc catcccgacc gatctccccg ggcttcgccg
     4921 ggttccagtc cacgccctgg atcccgttcc agttggatat ggaggcgtcc tggctgaact
     4981 ggtagtcgag ccatttgaag gccaacgcgg ggttcttgca ggctctggtg atcgccgcgt
     5041 cgctgccgtc cgtgtgggga gagtagggga agtagccgga gaactggact ccgttggggc
     5101 ccttcagggg tggcacgact tcataggcga agctctgcgg gctgtattcg ttggtggtca
     5161 tcatcccgcg gtgaccgacc attgccgcgc cgaccaggac gtggggaccc atgaccagct
     5221 gctgcagctg cgacaggttc tggacaaagg cctggggatc cagcagccct tccgcgtaaa
     5281 gctggtgcat gtacttcaga ccctcacgga actggggctg ggagaaggcc gggatgacct
     5341 tcccgctggc gtccacgttc atgcggttct ggccgtcgtc atagatgaag gcattcatca
     5401 gaaagccgtc cagcgtgctg tgccaggtgt tcagcgctcc ggagagcggg atctcgtcgg
     5461 cctggccgtc gccgttcggg tcaccggcct tgaacgcctt caggacgttc tcgaactccg
     5521 ccgtggtggt cggcattttc agattcagct tcttgagcca atccgtgttc atccacatct
     5581 tctctgccac ggagcagtga tagcactctt cccatgcgcc caacatgtag atctgcccat
     5641 ccgacaggac gctgtccgcc agatacgccg ggtcctgctg gatgacctgc ttgaggtaga
     5701 acccatgctg ctggatgagg tcattcaggg gaagcagcac gcccttgggc ccgccgtagg
     5761 tctcctgcca acccgaaggc atggcggcgc cgatgatcat gtccggcagt ttgccgctgg
     5821 acaggatgag cgcgatcttg gagctggaat ccgcggtctg gggcaccacc tggaagtcca
     5881 ggtggacgcc ggtcttctgc tccatccatt tcgtgtacgc gttggtggta atgtcggtga
     5941 tattgccaaa gggctcgatg agagccgtta acgtcgtggg tgtcttcacg agcggaagct
     6001 ctcccggcgc ggacaccgct gcctgggatg atgaggcacc ctctttctga ccgccagcaa
     6061 acagcggtcc aacgacgatg attgccacga tcaagacgca ccagccgatt cgtctggtca
     6121 tattctctcc tcctgaggaa ctgctcttgg atcggttgct ccgtcctgga gccaccccgt
     6181 gccgactcgg gccttcaacg gacgccgtct gccactcgcc acctccttcg cccctccggg
     6241 ctaacccttc aatgccccga tcatgacgcc tcgaacgaag tacttctgca cgaacggata
     6301 cacgcacagg accggcaggc tcgccacgat gatcacggcg tatttcatgg tcgcatagag
     6361 cagatgcagc ttgtcctcgg cttccacgct gatcgatccg cccttggtgt ccatggaatt
     6421 gaggatgagg atcttctgga gaatgagctg caggggaaag agctgctgtc ggttcagata
     6481 gagaaacggc cagaagaact gattccagat cccgacggca tagaacagga cgttgaccgc
     6541 cgtgattgcg cccgagagcg gcagcacgac gctggagaaa aagcgcagat cggaacagcc
     6601 gtcgatcgtt gccgcgtcca gaagagacgg cgagatggtg tactggtagt aggttcgggt
     6661 gatgatcacg ttccacgagg aaacggcgaa gggaaggatc atcgcccagc gcgtgtcgac
     6721 catgccgagg ctgcgaacca gaagataggt cgggatgatt cctccgctga agatcatggg
     6781 gaagaaaacg atgaacaggt agacgctgcg cccgcggaag tccgtcctgg acagcgggta
     6841 ggccgccagc agcgtaaggg cgacgctgac cagagtcccg gtaatcgtgt agaagatggt
     6901 gttgatgtac ccggtccaga tggaggcata ggtgagcgtc tcgttgtacg cggagaccga
     6961 cggtctgacc gggtacagcc agacggagcc cgcaatgacc tcattcggat cgctgaacga
     7021 gttgcttacg atgtacacca gcgggtagac cacgagcacg cagacaatcc cgaggaacac
     7081 gccgttcagc acacgaaagc ccacatctcc gccggttcga cgcatcgcca acgacgttcg
     7141 ggacacgctc tccccctcta ccacaggctg accccgccga gccttcgggc cacctggttg
     7201 gcccccagga ccaggacgag gttgatgacg gaattgaaga ggccgatggc ggccgcgtac
     7261 gagaactgag gaacaccgga catcccgacc ttgtagacgt acgtctggat gatctccgag
     7321 gacgtgaggt tcaacgggtt ctgcatcaga taggccttct cgaatccgag attcatgatc
     7381 tggccgaggg acaggatcag cacgatgacg accgtgggaa ggatccccgg gaagtccacg
     7441 tgcacggtcc gttggaagcg gctggcgccg tccacggtgg ccgcctcgtg cagctccggg
     7501 tcgacggagg ccagggcggc gagatagatg atgga
//
LOCUS       DASRZS010000301         4968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_939818, whole genome shotgun sequence.
ACCESSION   DASRZS010000301 DASRZS010000000
VERSION     DASRZS010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939818"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VFH83_07455"
     CDS             complement(<1..681)
                     /locus_tag="VFH83_07455"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HET6486240.1"
                     /translation="MSELEIQKLVAGGDGLAFADGKAVFVPGVLPGERVRVRVASRRR
                     DYDRAILEEVLVPSPARVKPPCRLAGICGGCDWLHIDPQEQRNQKLAILRESLRRTGH
                     VDLPDLAVEAGPELGYRNRVQLHRSSTGRLGYMAAESARVIEVEECPVAAPSVNTLFG
                     GTVPPPEGLDRFIAFGGDGWVAREGADDARDLEVVVAGKRILFSVGCFFQSNLAALQS
                     LVPYVLEGL"
     gene            complement(678..1220)
                     /locus_tag="VFH83_07460"
     CDS             complement(678..1220)
                     /locus_tag="VFH83_07460"
                     /inference="COORDINATES: protein motif:HMM:NF020483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HET6486241.1"
                     /translation="MSDPQSNGKVGERTQIRFLTPQNCRIHLGNLGALHVTVKGEGIF
                     GGIYTAYAFPVAYPNGYISIVQTAGDQDTEVGVIRDLSEFPEGDAALVRQALQRRYFI
                     HTVTRLKSITMQYNMLYLEVETDKGELAFFMRWAQDRAVDYGLTGKVLIDVDDNRYLI
                     PDLEKLSTRERVDFQRFIYW"
     gene            complement(1217..3580)
                     /locus_tag="VFH83_07465"
     CDS             complement(1217..3580)
                     /locus_tag="VFH83_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486242.1"
                     /translation="MSQNRSALLNHLLPADLQEALRRDGVDPGGLWLSAQTDLNLAGS
                     YEAVYLVADATRVLTVGLPHNGQKSTIRQIIQKDHLSEVRTRQGIGGGFVEALVDGIY
                     VEILAYSNARADTFHKAAKKLEDWRKGREVYIGPEDDEDPRKCPKCGMTLEFKGDICR
                     RCINQGAVASRVLRLMKPYSGYAAVMLTVLMVIIALQMVPARLIGLLVDNVVSVKAAS
                     QTALSLPLPLWGTVSRTILAAHPGLDQHILGLLFLVGVLFLTQVVMGAMNMVNGRLVS
                     RVGTQITSDLRKQLFRRLNQLSVDYYDRHNVGTLVSRVSYDTEAMKDFVRQAIQGLLA
                     QMVVLVVTGVMLFSISWKLALWTLLPAPIVIFSSTFYWRRIYPRYYRVWEGWSRMNGS
                     LSSILSGMRVVKAFGQENREEGRYGKTNDYVRDSTRGVEYTTSVFNPVMGLVFGMGGL
                     IVWVVGGRGVIQGQGLSLGSLMAFLNYLAMFYAPMTNLSQLTDWVTRFLTAAQRVFEI
                     MDTSPQVTPSARPRRLEDVKGHIVFDHVTFGYNRHEPVIKDVSFEIQPGEHLGIVGKS
                     GSGKTTLVNLLARFYDVEEGRILLDGEDVREIDPDDLRRAIGIVLQESFLFRGTIYAN
                     LTYGNSEATPDQVLAAAKAANAHDFIIRHPLGYDTYIGERGAGLSGGERQRASIARAL
                     LYDPKVLILDEATSNVDTESEQLIQEALTRFTAGRTSIAIAHRLSTLKTSDRIIVMEY
                     GQIVEMGTHEELLALGGLYHKLVKIQTELSREPNVDSLAVTKELTKQ"
     gene            complement(3588..4217)
                     /locus_tag="VFH83_07470"
     CDS             complement(3588..4217)
                     /locus_tag="VFH83_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET6486243.1"
                     /translation="MNHLDELIQRYPTLSSCQGAVRAAYDALARCYAAGGKVLICGNG
                     GSAADADHIVGELMKGFLSLRPLPEDARRRLEAADAEMGPTLARKLQAGLPAINLSAA
                     SALGTAFANDVSAELSYAQAALAYGKPGDVLWGISTSGNAANVRAALCAAHAAGMITL
                     GLTGKGGGKMARLCDVLIDVPATRTFEVQELHLPIYHCLCAMIEGRFFP"
     gene            complement(4266..>4968)
                     /locus_tag="VFH83_07475"
     CDS             complement(4266..>4968)
                     /locus_tag="VFH83_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6486244.1"
                     /translation="PGWDNVPLTDELERALGCRAYLQNDANACALAEWKYGAGRGVSS
                     MVFLTFGTGMGAGLILGGRLYEGVNDLAGEVGHIRLEEDGPLGYGKRGSFEGFCSGGG
                     IARLARDLAERSLAAGEPVAFCPDTRSLDLITTQAVGQAAEAGDPLAIEVLAKSGRYL
                     GKGLSILIDVLNPERIVIGSIFVRCERFLRPAMEREIGVEALAPAARVCAVVPAALGE
                     SLGDYACLSVAMQGS"
BASE COUNT          837 a         1646 c         1654 g          831 t
ORIGIN      
        1 aaggccttcc agcacgtagg ggaccaggct ctgcagggcc gccaggttgg actggaagaa
       61 gcagccgacg gagaagagga tccgcttgcc ggccaccacc acctcgaggt cccgagcgtc
      121 gtccgcgccc tctcgggcca cccaaccgtc gccgccaaag gcgatgaagc ggtccagccc
      181 ttcgggcgga ggcaccgtcc caccgaacag ggtgttgacg gagggcgcgg ccacggggca
      241 ctcctccacc tcgatcacgc gggcgctctc cgcggccatg tagcccagac ggccggtcga
      301 ggagcgatgg agctgcaccc ggttgcggta gcccagctcc ggccccgcct cgacggccag
      361 gtccggaagg tccacgtggc ccgtgcggcg aagcgactca cggaggatgg caagcttctg
      421 attccgctgc tcctgggggt cgatatgcag ccagtcgcag ccgccgcaga tcccggcgag
      481 ccggcacggc ggcttcaccc gcgcgggtga aggcacgagc acctcttcca ggatggcgcg
      541 gtcatagtcc cttcggcgcg aggccacgcg gacgcggacc cgctcccccg gaaggacacc
      601 gggaacgaag acggccttgc cgtccgcgaa ggccagaccg tcgccgccgg ccaccagctt
      661 ctggatctcg agctcgctca ccagtagatg aagcgctgga agtccacgcg ctcccgagtc
      721 gacagcttct ccagatccgg aatcaggtaa cggttgtcgt ccacgtcgat gagcaccttg
      781 cccgtcagac cgtagtctac ggccctatcc tgggcccacc gcatgaagaa cgcaagctct
      841 cccttgtcgg tctccacttc caggtagagc atgttgtact gcatggtgat ggacttgagc
      901 cgcgtcacgg tgtggatgaa gtaccgacgc tgcagggcct ggcgcaccag cgcggcgtcc
      961 ccctcgggga actccgagag gtcccggatg acgcccacct ccgtgtcctg gtctccggcg
     1021 gtctggacga tggagatata cccgttcggg taggccaccg gaaacgcgta ggcggtgtag
     1081 atgcctccga agatcccctc ccctttcacg gtgacgtgaa gggcgccgag gttccccagg
     1141 tggatgcggc agttctgcgg agtcaggaat cggatctgag tgcgctcgcc aaccttgccg
     1201 ttgctctggg ggtcgctcat tgcttcgtga gctccttggt gacggccaga gagtccacgt
     1261 tcggctcgcg ggagagctcg gtctggatct tcaccagctt gtgatacagc ccgcccagcg
     1321 ccagcagctc ctcgtgcgtc cccatttcca cgatctggcc atactccatg acgatgatgc
     1381 ggtcggaggt cttcagcgtg gaaagccggt gcgcgatggc aatggacgta cggccggccg
     1441 tgaaccgggt cagggcctcc tggatgagct gctcgctctc ggtgtccacg ttggaggtcg
     1501 cctcgtcgag gatgagaacc ttcggatcgt agagcagcgc tcgggcgatg gaggcccgct
     1561 gccgctcgcc accggagagg ccggccccgc gctcgccgat ataggtgtcg tagcccagcg
     1621 ggtggcggat gatgaagtcg tgggcgttgg cagccttggc ggcggccagt acctggtccg
     1681 gggtggcctc gctgttgccg taggtgaggt tggcgtagat ggtgccgcgg aagaggaacg
     1741 actcctggag cacgatcccg atggcgcggc gcaggtcatc cggatcgatc tcccgcacgt
     1801 cctccccgtc cagcaggatg cgcccttcct ccacgtcgta gaaccgggcc agcaggttga
     1861 ccagggtcgt cttgcccgag ccgctcttgc cgacgatccc caggtgctcg cccggctgga
     1921 tctcgaagct cacgtccttg atcaccggct catggcggtt gtagccaaag gtcacgtggt
     1981 cgaagacgat gtggcctttc acgtcctcga gccggcgggg acgcgccgag ggcgtgacct
     2041 ggggggaggt gtccatgatc tcgaacacgc gctgggcggc ggtgaggaac cgggtgaccc
     2101 agtcggtgag ctgggagagg ttggtcatgg gcgcatagaa catcgccagg taattgagga
     2161 aggccatcag ggagcccagg ctgaggccct gcccctggat cacgccccga ccgccgacca
     2221 cccagacgat gaggcccccc atgccgaaca ccagccccat gaccgggttg aaaacgctcg
     2281 tggtgtactc cacgccgcgc gtggagtccc gcacgtagtc gttggtcttg ccgtatcggc
     2341 cttcctcccg gttctcctgg ccgaaggcct tcaccacacg catgcccgag aggatggagc
     2401 tgagggagcc gttcatgcgg ctccaaccct cccagacgcg gtagtagcgg ggatagatcc
     2461 ggcgccagta gaacgtgctg gagaagatga cgatgggcgc cggaagcagc gtccataggg
     2521 cgagcttcca cgagatgctg aagagcatga cgccggtgac caccagcacg accatctgcg
     2581 ccagcagtcc ctggatggcc tgacgcacga agtccttcat ggcctccgtg tcatagctca
     2641 cccggctcac cagggtgccc acgttgtgtc ggtcgtagta gtccacgctg agctggttga
     2701 gcctgcggaa gagctgcttg cgcaggtcgc tcgtgatctg cgttccaacg cggctcacca
     2761 gccggccgtt caccatgttc atggcgccca tgaccacctg ggtcaggaag agcacgccaa
     2821 ccaggaagag gagcccgagg atgtgctggt ccagaccggg gtgggccgcc aggatggtgc
     2881 gggagacggt cccccagagc gggagcggga ggctgagcgc cgtctggctt gccgccttca
     2941 cgctcaccac gttgtccacc agcaggccga tgaggcgggc cgggaccatc tgcagcgcga
     3001 tgatcaccat gagcacggtc agcatgacgg ccgcgtaccc gctgtagggc ttcatgagcc
     3061 gcaggacccg gctggcaacg gcgccctggt tgatgcatcg gcggcagatg tcccctttga
     3121 actccagggt catcccgcac ttggggcact tgcgagggtc ctcgtcgtcc tcgggtccga
     3181 tgtagacctc gcgtcccttg cgccagtcct cgagcttctt ggcggccttg tggaacgtgt
     3241 ccgcccgcgc attggagtag gccaggatct cgacgtagat cccgtccacc aacgcctcca
     3301 cgaagcctcc gccgatgccc tgacgggtcc tgacctcgga gagatggtcc ttctggatga
     3361 tctggcggat cgtgctcttc tggccgttgt ggggcagccc aacggtgagc acgcgggtgg
     3421 catccgccac caggtacacc gcctcgtagg aacccgccag gttgagatcg gtctgggccg
     3481 agagccagag gccgccggga tccaccccgt ctcgacgcag ggcttcctgc agatcggcgg
     3541 gcaagaggtg gttcagaagc gcggaccggt tctggctcat gggctcctca cgggaagaag
     3601 cggccttcaa tcatggcgca cagacagtga tagataggga ggtgaagctc ctggacctcg
     3661 aaggtgcggg tcgcggggac gtcgatgagc acgtcgcaga gccgggccat cttgcctccg
     3721 cccttgccgg tcagacccag ggtgatcatc ccagcggcgt gagccgcgca gagcgccgcc
     3781 cgcacgttgg cggcgttccc ggaggtgctg atgccccaga ggacgtcgcc gggctttccg
     3841 tatgccagcg ccgcctgggc gtaggaaagc tcggcgctca cgtcgttggc aaacgcggtt
     3901 cccaacgcgg aggccgcgga gagattgatt gccggcaggc ctgcctggag cttccgggcc
     3961 agcgtgggac ccatctcggc gtccgctgct tccagtcgtc ttcgcgcgtc ctcgggcagg
     4021 ggtcgaaggc tgaggaagcc cttcatcagc tcgcccacga tgtggtcggc gtcggccgca
     4081 cttccgccgt tgccgcagat caggaccttc ccgccggcgg catagcagcg cgcaagggcg
     4141 tcgtaggcgg cccgaacggc cccctggcag gaagagaggg ttgggtatct ctggatcagc
     4201 tcgtccaggt ggttcatctc aggctccttc cgccgccacg gatcaagtcc ctccatggcc
     4261 gcggatcatg atccctgcat ggccacggac agacacgcgt agtccccaag gctctccccc
     4321 agcgcagcgg gcactaccgc gcacaccctt gcggctgggg caagggcctc gacgccgatc
     4381 tcccgctcca tggcgggcct caagaagcgc tcgcatcgaa cgaagatgct gccgatcacg
     4441 atccgctcgg ggttcagcac gtctatgagg atggagaggc ccttgccgag gtaccggccg
     4501 ctcttggcca ggacctcaat ggccagcgga tcccccgcct cggcggcctg gcccacggcc
     4561 tgggtggtaa tgagatccag ggacctggtg tcaggacaga aagcaaccgg ctctccggcg
     4621 gcaaggctcc tctccgccag atcccgcgcc agccgggcaa tcccgccgcc gctgcagaat
     4681 ccttcgaagg agccgcgctt gccgtagccc aacggcccgt cctcttccag acggatgtgt
     4741 ccgacctcgc cggccagatc gttgacccct tcgtagagcc tgccgcccag gatgagaccg
     4801 gcgcccatgc ccgtgccaaa ggtgaggaac accatggagc tcacgccgcg gcccgcgcca
     4861 tacttccact cggccagggc gcaagcgttg gcgtcgttct gaagataggc acggcagcca
     4921 agcgctcgtt ccagctcgtc ggtaagggga acgttgtccc agcctggg
//
LOCUS       DASRZS010000302         5636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_556491, whole genome shotgun sequence.
ACCESSION   DASRZS010000302 DASRZS010000000
VERSION     DASRZS010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556491"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="VFH83_07480"
     CDS             <1..1679
                     /locus_tag="VFH83_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET6486245.1"
                     /translation="SRACLPKKGHYWMKSVPGLDLSKGMVDQFIPGNDRFYADPSEVG
                     PWRNPTDIDVVGDHYWVEERLPVESIDSKTGLVRCRRRSVFVLRDDLGGKLCRYRFEN
                     VAEALTEPGEWYLDRTSGSLTYLALPGEEPGKTLVTAPCTTELLALRGTEANPVRGVT
                     VENLTFECTDWQQPPAGSLEGFEDAGLLRADEAYGAATQAACNLGGVISGEYAQDCTL
                     RDLTIRNTGHYAVVLGRGCRRVTVAGNRMEDLGAGGVRLNGGNAYAEKSAHNGEHQIT
                     DNEILHGGRVFLSAVGVLLMHSYGNLVAHNHIQDFYYTGVSCGWVWGYDASVSRDNRI
                     EYNHIHDIGQGVLSDMGGIYTLGVQPGTVLRGNRIHDVQQRNYGGWCIYLDEGSSHIL
                     VEGNVCYRAGTQVFNQHYGRENVVRNNLFAFGGHTVVSTGRLEDHNSFTLTNNILVTD
                     GKPVIAIAAARGEGRLGFRADSNLVWDTKGAPCFCAILATEKPGDARRWTEAAWKETR
                     NDLFSIVADPRFADPAKDDFTLKPDSPAARIGFTAFDLSRAGPRKGTTGA"
     gene            1713..3377
                     /gene="lnt"
                     /locus_tag="VFH83_07485"
     CDS             1713..3377
                     /gene="lnt"
                     /locus_tag="VFH83_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET6486246.1"
                     /translation="MSLRSRLILLVLLSSVLLPLALPNELLGAALKALGLRPDQSFYW
                     GDAILGLVCIAPLFYAIVEAPSFGFASILGMIFGAVSTLLASYWLMFFQNFSVWTMGG
                     TVLGYVGYNAVLFPFLRGFSNISGRFRPFVLAAAWAVYEYFKSIGFLGYPWGLVADPA
                     TGVLPLIQIADVTGIWGLSFLMALGNALVAEYALNGYRLRFLRQAVFSAVLVAAALVY
                     GVVRLATPIPSHGTASMLLVQQNMDPWQEGVAAAESVRINAQLTIDGVSRAKDPPDLA
                     VWSESSVSTPELIVVPGNRFNREKNDLVPMIQRAGVPILFGGVVEVSRAQQEYMNASV
                     LVDGRGAILDTYGKIHPVPFAESIPFFEYPAVRRFFRDVVGLWNEWTMGKRFTIYRVP
                     LAAGGQLAFGTPICFEDAFADVCRGFVLRGADMLVNITNDSWSKTWSAEIQHFQVARL
                     RAVENRRVLVRSTNGGVSGVVGPWGEIRAEMPLFQSTWGLVRVPVYESGTLTLYTRLG
                     DWFPQALIAVLFVFLALSVALSAPWADRPPTGRRTVGAPWAWRGSP"
     gene            complement(3325..3597)
                     /locus_tag="VFH83_07490"
     CDS             complement(3325..3597)
                     /locus_tag="VFH83_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET6486247.1"
                     /translation="MAVNLYVGNLPYRIDEDSLRQLFTPYGGVETSKVIKDRQTGRSK
                     GFGFVEMTTQSEAEAAIKALNEQPLDGRNIKVSLAKPKEPRRFDDQ"
     gene            complement(3819..4715)
                     /locus_tag="VFH83_07495"
     CDS             complement(3819..4715)
                     /locus_tag="VFH83_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET6486248.1"
                     /translation="MMELERFAAPVRAELEEADARIGALVDGICRDGMLPDLCEQFVQ
                     PAVRHLFRTSGKLLRPLLVLLSARASGVREVAGIGGSASQEALVRAAAGVELLHTASL
                     AHDDMVDGSAERRGSPSLHVAYGSTTAILVGDVFYSRFFQELTCLPGTAPAARLRLLQ
                     VFLSVTGRMCTGEILEEQLRAEGGIPSLDVYLDITDAKTAGLVSACCLAGGLLAGADE
                     SACQSLAAYGKAVGLLFQITDDLMDGDGAYPDRMAMTIEASKNRIAALTSILDLPPGD
                     ATDALRRLPAFLYARAGLVSAS"
     gene            complement(4743..5303)
                     /locus_tag="VFH83_07500"
     CDS             complement(4743..5303)
                     /locus_tag="VFH83_07500"
                     /inference="COORDINATES: protein motif:HMM:NF017933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF981 domain-containing protein"
                     /protein_id="HET6486249.1"
                     /translation="MFIDFLTLMLTNMAVALLLFALYMAFFFEKDSKKMVPGFLIVGA
                     IALITGFRMVFTWPLPGSFNIAYGEPTILLGAFFFMAGLAINFGWDLLTVGIYSAVAG
                     AAVILLGSRILDLKMGSNPPEAFLAFLGSGITAILALPSIALPKMKWIRWITALAAVV
                     TFAGWIYVGFSAYWGHLDAFSKWAPR"
BASE COUNT          966 a         1824 c         1861 g          985 t
ORIGIN      
        1 gcagccgggc atgccttccc aagaagggac actactggat gaagagcgtc cccgggctcg
       61 acctctccaa gggcatggtc gatcagttca tccccgggaa cgatcggttc tatgcggacc
      121 cctccgaggt ggggccgtgg cggaacccca cggatatcga tgtagttgga gaccattact
      181 gggttgagga gcggctgccc gtcgagtcca tcgactcgaa gaccggcctg gtacggtgcc
      241 gacgccgaag cgtcttcgtg ctgcgcgacg atctgggtgg caagctctgc cgctatcggt
      301 tcgagaacgt ggcggaagcg ctcaccgagc cgggcgaatg gtacctggac cggaccagcg
      361 gctcccttac ctacctggcg ctcccgggtg aggagcccgg aaagacgctg gtcaccgccc
      421 cgtgcactac cgagcttctg gctcttcggg ggaccgaggc caatcccgtg cgcggagtga
      481 ccgtggagaa cctcaccttc gaatgcacgg actggcagca gcctccggcg ggaagcctgg
      541 agggcttcga ggacgccggc cttctgcgcg ccgacgaagc gtacggagcc gccacgcagg
      601 ccgcctgcaa cctgggcggt gtgatcagcg gggagtacgc ccaggactgc acgctgcgcg
      661 acctcaccat cagaaacacc gggcactacg cggtggtcct gggccgcggg tgccggcgcg
      721 tcacggtggc gggcaaccgt atggaggacc tgggcgccgg cggcgttcgt ctgaacggcg
      781 ggaacgcgta cgccgagaag agcgcccaca acggggagca ccagatcacg gacaacgaga
      841 tcctccacgg cggccgggtc ttcctgagcg ccgtcggcgt gctcctgatg cacagctacg
      901 gcaacctggt ggcgcacaac catatccagg acttctacta caccggcgtc tcgtgtggtt
      961 gggtctgggg atacgacgcc agcgtcagtc gggacaatcg aatcgagtac aaccacatcc
     1021 acgacatcgg ccagggcgtc ttgtccgaca tgggcggcat ctacaccctg ggagtgcagc
     1081 ccgggacggt gctgcgcggc aaccggatcc acgacgtcca gcagcgcaac tacggcggct
     1141 ggtgcatcta cctggacgag ggaagctccc acattctggt ggaaggaaac gtctgctatc
     1201 gggcgggcac gcaggtgttc aatcagcact acggccggga aaacgtggtg cggaacaacc
     1261 tcttcgcctt cggcggtcac acggtcgtgt ccaccggcag gctcgaggac cacaactcct
     1321 tcacgctcac caacaacatc ctggtgacgg acgggaagcc cgtgatcgcc atcgccgcgg
     1381 cgcgaggcga gggacggctc gggttccgcg cggacagcaa cctggtctgg gacaccaagg
     1441 gcgcgccgtg cttctgcgcc atccttgcta ccgagaagcc tggcgacgcc cgccggtgga
     1501 ccgaggcggc gtggaaggag acgcgcaacg acctgttcag catcgtcgcg gacccgcggt
     1561 tcgccgaccc ggccaaggac gacttcaccc tgaagcctga ctcgccggcg gccagaatcg
     1621 ggttcacggc gttcgacctc tcgcgcgcgg ggccgcggaa gggaacaacc ggcgcttgaa
     1681 cctccgtgcg ggtagggcta cctttccgcc tgatgagcct cagaagtcgc ctgatcctgc
     1741 tggtgctgct ttcatccgtg ctgcttccgc tcgctcttcc caacgagctc cttggtgcgg
     1801 ccctcaaggc gctgggtctg cggcccgacc agagcttcta ctggggggac gcaatcctgg
     1861 gcctggtgtg catcgcaccc ctattctacg caatcgtgga ggctcccagc ttcggctttg
     1921 cctccatcct ggggatgatc ttcggcgcgg tctcgaccct tctggccagc tactggctga
     1981 tgttcttcca gaacttctcc gtctggacca tgggcggcac ggtgctgggg tacgtcggct
     2041 acaacgcggt cctgttcccc ttccttcgag ggttctcgaa catctcgggc cgcttccgcc
     2101 ccttcgtcct ggctgccgcg tgggccgtct acgagtactt caagtccata ggcttcctgg
     2161 gctacccgtg ggggctggtg gcggacccgg caaccggggt tcttccgttg atccagatcg
     2221 cggacgtgac cggcatctgg ggactgtcct tcctcatggc gctgggcaac gcgctcgtgg
     2281 ccgagtacgc cctgaacgga taccgactgc ggttcctgag acaggcggtg ttctccgcgg
     2341 tactggtggc ggccgccctg gtctacggcg tcgtgcgcct ggcgaccccc atccccagcc
     2401 acgggaccgc ctcgatgctc ctggtgcagc agaacatgga cccgtggcag gaaggggtgg
     2461 cggccgcgga gtccgtgcgg atcaacgctc agctcacgat cgacggggtg agccgcgcga
     2521 aggacccacc ggatctggcc gtgtggagtg agagctcggt ctcgacgccg gagctgatcg
     2581 tggtgcccgg caatcgcttc aatcgggaga agaacgacct ggtcccgatg atccagaggg
     2641 cgggagtccc gatcctcttc ggcggtgtcg tggaggtaag ccgcgcgcaa caggagtaca
     2701 tgaacgcatc cgtgctggtg gacgggcgcg gcgccatcct ggacacgtac ggcaagatcc
     2761 accccgtgcc ctttgccgag agcatcccgt tcttcgagta ccccgcggtc cgccgcttct
     2821 tccgggacgt ggtggggctc tggaacgagt ggaccatggg gaagcggttc accatctacc
     2881 gtgttcccct cgccgccggc gggcaactgg cgttcgggac gcccatctgc ttcgaggacg
     2941 cctttgccga tgtgtgccgc gggttcgtcc tgcgcggggc ggacatgctg gtgaacatca
     3001 ccaacgactc gtggtccaag acgtggtccg cggagatcca gcacttccag gtggcacggc
     3061 tccgcgcggt ggagaaccgc cgggtcctgg tccgctccac caacggcggc gtctccggcg
     3121 ttgtggggcc gtggggagag atccgcgccg agatgccgct cttccagagc acctgggggc
     3181 tggtcagggt gccggtctac gagtcgggga cgctcacgct gtacacccgg ctgggagact
     3241 ggttccctca ggcgctgatc gcggtgctgt tcgtcttcct ggcgctgtcc gtggcgctct
     3301 cggcgccctg ggccgaccgt ccgcctactg gtcgtcgaac cgtcggggct ccttgggctt
     3361 ggcgaggctc accttgatgt tcctgccatc gaggggctgc tcgttcaggg ccttgatcgc
     3421 ggcctccgcc tcggactgcg tggtcatttc gacgaagccg aagcccttcg agcgcccggt
     3481 ctgccggtcc ttgatcacct tgcttgtttc gacgccgccg tacggcgtga acaattgacg
     3541 cagggaatcc tcgtcaatcc ggtagggcag gttccctacg tagagattca cggccataga
     3601 ctctcctttt cagcggggaa cggtgagaca tggttctggt ggtacaacag ggagcacacg
     3661 taatcctgac cagttcgcgc tgattttagc ggctaacccg gccgggcgca actttttttt
     3721 tactgtgtct gtcgattgcc ttgtacgtta tgccgttggt cgaggaggag cgctccctgg
     3781 cgatctctgg aacgattgcg ctttcgcctg atcggcgatt aactggcgct gacgagcccg
     3841 gcccgggcat agaggaaagc cgggagcctc cgtagcgcgt cggtcgcgtc gcctggcggc
     3901 agatccagga tgctggtgag agcggcaata cggttcttgg acgcctcgat ggtcatggcc
     3961 atgcggtccg ggtaggcccc atcgccatcc atcaggtcat ccgtgatctg aaagagaagc
     4021 cccaccgctt tgccgtaagc agccagagac tggcaggcgc tctcatccgc tccggccaga
     4081 aggccgccgg cgaggcagca ggccgatacc aggcccgcgg ttttagcgtc cgtgatgtcc
     4141 aggtacacgt ccagggaggg gatcccgcct tccgcccgga gctgctcctc caggatctcc
     4201 cccgtgcaca tccgacccgt gacggacagg aagacctgaa gcagtcgcag gcgggcggcg
     4261 ggcgcggtgc caggcaggca ggtcagctcc tggaagaagc gggagtagaa cacgtctccc
     4321 accaggatag cggtggtgct gccgtaggcc acgtggaggc tcggcgaacc ccgtcgttcc
     4381 gccgagccat ccaccatgtc gtcatgcgcc agggacgccg tgtggagcag ctccaccccg
     4441 gcggcggcgc ggaccaatgc ctcctgcgag gcggacccgc cgatgccggc cacctcccgg
     4501 acacccgagg cccgcgccga caagaggacc aggagcggcc gcaggagctt accgctggtc
     4561 cgaaagagat gccgcacggc gggctgcacg aactgttcgc agaggtccgg gagcatgccg
     4621 tcccggcaga tcccgtccac cagagcgcca atcctggcgt cggcttcctc gagctccgcg
     4681 cgaaccggcg ccgcgaacct ctcgagctcc atcatgcgcc cgccgccgct tcgtccccta
     4741 ggctaccggg gcgcccactt ggagaacgcg tccagatggc cccagtaagc gctgaaccca
     4801 acgtagatcc agccggcgaa ggtgacaacg gcggccagag ccgtaatcca gcgtatccac
     4861 ttcatctttg ggagagcgat cgacggaagc gccaggatcg cggtgatgcc gcttcccagg
     4921 aacgccagga acgcctccgg agggttcgat cccatcttga ggtcgaggat ccggctgcca
     4981 agcaggatga cggcggctcc ggcaaccgcg gagtagatcc ccaccgtgag gaggtcccac
     5041 ccgaagttga tcgccagtcc tgccatgaag aagaacgctc ccagcaggat ggtcggctca
     5101 ccatacgcga tgttgaacga tccgggcagc ggccaggtga ataccatccg gaagccggtg
     5161 atcagggcga tggctcccac gatcaggaag cccggcacca tcttcttcga gtccttctcg
     5221 aagaagaagg ccatgtacaa cgcgaagaga aggagcgcta cagccatgtt ggtcagcatg
     5281 agagtcagga aatcgatgaa catccgtcac ctcctgtgcc ccgctgggcg tgcatcaact
     5341 ccgggccagg gaacatgccc cgcgcccctt tcaccgaagc aacatctttg cgtgctggaa
     5401 atagaatagt attttgatag ggtacttgta aagctttttt tggcagaggg ccgatccgtc
     5461 accgtcaaac aggcgccgga atccggccgg gtgggcgtac gccctggcgg ctacccacgg
     5521 gaggtggtgc ccatgaacgt agcggtgtgg agaaaggctc tgcgggtgat cccctcggtc
     5581 acccgggagg agtgggccgg gctggacctc ctggcccgat ggctgatctc ttcccg
//
LOCUS       DASRZS010000303         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5558588, whole genome shotgun sequence.
ACCESSION   DASRZS010000303 DASRZS010000000
VERSION     DASRZS010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5558588"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2041
                     /locus_tag="VFH83_07505"
     CDS             <1..2041
                     /locus_tag="VFH83_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486250.1"
                     /translation="LRTALDWLNGTARFWRSRLGQLTVGAPEGGPEAELSRETWVRCI
                     LDNFNCLQTDASGNLLSHWQGAPSHGYGTVWGIDIEPTAVSMMQVCPELGRQVLLFSL
                     NRTRPPRGEPDNSIPILVAPLVIARRYLEASGDMDLFQRAEVTSALRGIAEELERFRA
                     KGERLYSSRYSSDGPVGRRYDFGTNVKVWYALEGLSAILGHTSLKADAERYRTMASEI
                     RQAIERTMIADGPFGPQVTGGTNLSEAPAGFYLPEGVPYYDGEDTSSMLAPVYGIFDY
                     RYAPWVNYHRFSRSLWCPNYDPEFGVLRWSPGEFSNGALDGTAWFSRLAGSVTRAEMR
                     EAMTALGTAGADTATGSVFWWPHGLEYRRSLTRCSQGQGAWAWQYLQQWLGVRVDATS
                     RTLTISPQGFLTEIEWDGFRCGPARFDLRWKEDGSGAVLRVRNTGADSWTVRVGIRAP
                     GSGADGAITWQNRPVASGQEAKFEQTHAARQDSPAQAGMTEAAIVAKEAAALGDKDGV
                     LFRRYGPARLWGAWDSASLWIPVQMPLAMRFVLVNGTPNDWSDVRVELSCPDGWKAQA
                     RLPMHWPEPSRLAPGTVRLELGALDSRHRTVAAFWLQGPNRYDTSVINREVSFHTVSQ
                     PGPGILLPGLDVSAEQEVSLTAELSARAADGREIRRTLRVPIRVVPAERLTGKGG"
     gene            2136..>2454
                     /locus_tag="VFH83_07510"
     CDS             2136..>2454
                     /locus_tag="VFH83_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486251.1"
                     /translation="MKYALPVVCICLLALSACATTSTAVYQSNAPEVRGERAISSNIE
                     LVKEVELEGSILGVFYSREVYASGDAQLVLFTLRPPSGGFGVKSGQGTMDLYDLGNPS
                     SLSV"
BASE COUNT          392 a          832 c          832 g          398 t
ORIGIN      
        1 cctgagaacc gccctggact ggctcaacgg caccgcgcgt ttctggcgct cccgtctggg
       61 gcagctcacc gtcggcgctc ccgaaggcgg cccggaggcc gagctcagcc gagagacctg
      121 ggtacgctgc atcctggaca acttcaactg cctgcagacc gacgccagcg gcaacctgct
      181 ctcccactgg cagggcgcgc cctcccacgg gtacggcacg gtctggggga tcgacatcga
      241 gccgacggcc gtgagcatga tgcaggtatg ccccgagctg ggccgccagg tcctgctctt
      301 ctccctcaac cgtacccgtc cgccccgcgg cgagccggac aactccatcc caatcctggt
      361 agcccccctc gtcatcgccc gtcgctacct ggaggcttcc ggggacatgg acctgttcca
      421 gcgcgcggag gtgacctcgg cgctgcgcgg catcgcggag gagctggagc gattccgcgc
      481 caagggcgag aggctgtact cctcgcgcta ctccagcgac ggcccggtgg ggcggcggta
      541 tgacttcgga accaacgtga aggtctggta cgccctcgaa gggctgtcgg ccatcctggg
      601 ccatacgtcg ttgaaggcgg atgccgaacg ataccggacg atggcgtcgg agatccgcca
      661 ggccatcgag cggaccatga tcgccgacgg gcccttcggt ccccaggtga ccggtggaac
      721 caacctgtcc gaggccccag ccggcttcta tctgccggaa ggcgttccct actacgacgg
      781 ggaggacacg tcgagcatgc tggctcccgt ctatgggatc ttcgactacc gttacgcgcc
      841 atgggtgaac taccaccgat tctcccggtc gctgtggtgt ccaaactacg atccggagtt
      901 cggcgtgctc cgctggagcc cgggcgagtt ctccaacgga gcgctcgacg ggaccgcgtg
      961 gttctcgcgg ctcgcgggaa gcgtgacccg cgccgagatg cgggaggcca tgaccgcgct
     1021 ggggacggca ggcgccgaca cggcaaccgg gagcgtgttc tggtggcccc acggcctgga
     1081 gtaccgccga tccctcacac ggtgcagcca aggtcagggc gcgtgggcgt ggcagtacct
     1141 ccagcagtgg ctgggcgtgc gcgtggatgc gacgagccgc accctcacca tctcacccca
     1201 gggctttctc accgagatcg aatgggatgg ctttcgctgc ggcccggctc gattcgacct
     1261 gcggtggaaa gaggacggtt ccggcgccgt gcttcgggtc cgcaacacgg gcgccgattc
     1321 gtggaccgtc cgggtaggga tccgcgcccc gggaagcggc gccgatggcg ccatcacctg
     1381 gcagaaccgt ccagtggcgt cggggcagga ggcgaagttc gagcagaccc acgcggcccg
     1441 gcaggactcg cccgctcaag ccggcatgac ggaagcggcg atcgttgcca aggaggccgc
     1501 ggctctcggg gacaaggacg gcgtgctgtt ccgccgctat gggcccgccc ggctctgggg
     1561 cgcctgggac tccgcctccc tgtggatccc cgtccagatg ccgttggcca tgcgcttcgt
     1621 cctggtgaac ggcacgccaa acgactggtc ggacgtacgg gtcgagctgt cctgcccgga
     1681 cgggtggaag gcgcaggcgc gcctgcccat gcactggccc gagccgtcgc ggctggcccc
     1741 cggtacggtg aggctggagc tcggcgcgct ggattcccga caccggacgg tcgccgcctt
     1801 ctggcttcag ggtccgaacc gatacgacac ctccgtcatc aatcgggagg tctcattcca
     1861 cacggtgagc cagccgggtc ccggcatcct gctgcccggt ctggacgtct ccgccgagca
     1921 ggaagtctct ctgaccgctg agctcagcgc cagggcggcg gacggccggg agatccgccg
     1981 gaccctccgg gtccccattc gcgtcgtgcc ggcggagcgt ctgaccggca aaggaggctg
     2041 agaaggggac gcggtgaacg ggctgcctgc gagatagatt atctctcgtt ccgatatacg
     2101 aatcgtccgg accatctcag aaaggagccc gaatcatgaa gtacgcactg ccggtggtct
     2161 gcatctgcct gctggccctc tccgcgtgcg ccacaacgag cacagcggtc taccagtcca
     2221 acgcacccga ggtgcgtggg gaacgcgcca tctcatccaa catcgagctg gtgaaagagg
     2281 tggagctgga aggctccatc ctgggtgtct tctactcgcg agaggtgtac gccagcggag
     2341 acgcccagct tgtcctgttc acgctccgtc ctccgtcagg cggttttggg gtcaagtccg
     2401 gccaggggac catggacttg tacgacctgg gcaacccctc gtccttgagc gtgg
//
LOCUS       DASRZS010000304         4443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3249046, whole genome shotgun sequence.
ACCESSION   DASRZS010000304 DASRZS010000000
VERSION     DASRZS010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3249046"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VFH83_07515"
     CDS             complement(<1..962)
                     /locus_tag="VFH83_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486252.1"
                     /translation="MRLVPLLEEAWLAIRTNRSRTALTVLGIVIGVGSVISVVGVGDG
                     AKKVVGDLLGRFGSTSLIVAPNFTAIRESKGKYRFEEITRDDIETINTTASAVRAVTP
                     QVQMDVDVKVAGTTQKATLIGTMHQYLDASSLTVRNGRFLDVDDDVFMRKVGVLGADL
                     AVKLFGTQNPIGSFVRINNVVDVQIVGVLEKESQSFISTVSDFDTSNNNRLFVPTSAI
                     ERVAGSSQIYFLIGEAVATDQIDDAEQQILTILDKSHGKWNGTVEKYIVQEMGTVVAM
                     IDTATGTLTAAISIIAGIALLVAGIGIMNIMLVSVKERTREIGVR"
     gene            complement(966..2183)
                     /locus_tag="VFH83_07520"
     CDS             complement(966..2183)
                     /locus_tag="VFH83_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486253.1"
                     /translation="MNWTEGFRESADMIRHNKLRTILTMLGMNIGVASVIAVMATGFM
                     GRTAIMKGIENIGSSLLWVQANRDAYPTGKDVLLMKPEDLSILTGLVQDAWVSPNLRG
                     NYSFGYRGDEQAAAVYGVWPDYQRMWGRSLAAGRYVSAADVRDRRRVVVLGRNTALAF
                     FPTEEAALGQSMSLAGSQFDVIGVMTKKERQPVDDGSDDDTAFVPYEVLSDMTNWDQY
                     GGPRVNRIYFKVHQVTDLDFVSGQVAQYLDHAYGTVDGKPRFTVRRAEDSIQTTNRVF
                     EIITTVITLIAGISLVVGGIGIMNIMLITVIERTREIGIRKALGAKRRDILAQFLIES
                     VIICLIGGGIGILFGMAITAVISIAQRWGYLLPWGAILVGLGVSISIGLFFGIYPALK
                     AARLDPVVALTQE"
     gene            complement(2180..2887)
                     /locus_tag="VFH83_07525"
     CDS             complement(2180..2887)
                     /locus_tag="VFH83_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486254.1"
                     /translation="MIALENVRKIYRLGKLEVPALQGVDLRIEEHEFAAIMGPSGSGK
                     STLMNIVGCLDRLTTGRYVLDGIDVSDMGDNDLAEVRNRRIGFVFQTFNLLPRMSSLR
                     NVELPLIYSGISKRERRDRAMAALKQVGLENRALHRPNELSGGQRQRVAIARALINRP
                     AILLADEPTGNLDSRTGEEIMAIFQQLNDQGVTIILVTHEPDIAACSSRIIALRDGLI
                     TRDEPVNARRAMDGVPT"
     gene            complement(2893..4197)
                     /locus_tag="VFH83_07530"
     CDS             complement(2893..4197)
                     /locus_tag="VFH83_07530"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6486255.1"
                     /translation="MKRRGVLVVIVTILVLVAAVIVLNLTLRGSRYETAIPVRAYLVN
                     RGSLQDRVSGNGTFVPKDAFTVTSQVSAEVRSIRVAENDRVQAGDVLLKLRDDDYVLA
                     AQKMRASLDATRKSIRQSLVTLRAQFRSATSAVSDAQRTFSKSKELFAAKAVSQESYQ
                     QASDAVDTAQVNLQSAREQLDLRCGLPLDATPPLDATQDDKIIEASPEVEQALLSLQS
                     AQDTVTRCTITAPAAGVVTDVKPSVGDQVSPSTPLVKIETLDDMLARIQIDEVDIGKI
                     RVGQPAEITSDSLIGLTLTGTVDTISPVITSVGSTRVSMVDVKVDPASRKTSDGSATL
                     RSGASCTARIVTSIKENVLLVPLSSFVTEENVTTVYLLQPTGKKNAAQADIYQLARRE
                     IGTGSSDVNSIEVTKGLAEGDRIVAGNLKLIREGILVTVRQD"
     gene            complement(4194..>4443)
                     /locus_tag="VFH83_07535"
     CDS             complement(4194..>4443)
                     /locus_tag="VFH83_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486256.1"
                     /translation="SLDEKVTLLTQSLSLAQRRLTTEQSLLSLGKSTDVNVAAKEAEV
                     DLSANDLWRARADLLLTVLDLESMAGEDLAPILEGTHS"
BASE COUNT          735 a         1443 c         1448 g          817 t
ORIGIN      
        1 cgcacgccaa tctcccgggt ccgctccttc acggagacca gcatgatgtt catgatcccg
       61 atcccggcca ccaggagcgc gatgccggcg atgatcgaga tggccgcggt gagcgtgccg
      121 gtggccgtgt cgatcatggc caccacggtg cccatctcct ggacgatgta cttctccacg
      181 gttccattcc acttgccgtg gctcttgtcc aggatcgtca ggatctgctg ctcggcgtcg
      241 tcgatctggt ccgtggccac ggcctcgccg atcaggaagt agatctggga cgatccggcc
      301 acgcgttcga tggctgaggt ggggacgaac agccggttgt tattggacgt atcgaagtcc
      361 gagaccgtgg agatgaagga ctggctctcc ttctccagca ccccgacgat ctgcacgtcc
      421 accacgttgt tgatgcgcac gaaggagccg atggggttct gcgtgccgaa gagcttcacg
      481 gcaaggtccg cgcccagcac gcccaccttc cgcatgaaca cgtcatcgtc aacatccagg
      541 aaccgaccgt ttcggaccgt gagggagctg gcgtccaggt actggtgcat ggtgccgatg
      601 agggttgcct tctgcgtggt ccccgccacc ttcacatcca cgtccatctg gacctgcggg
      661 gtgacggccc gcaccgcgga ggcggtagta ttgatggtct cgatgtcgtc ccgagtgatc
      721 tcctcgaacc ggtactttcc cttgctctcc cgtatcgcgg tgaagttcgg cgccacgatg
      781 agagaggtgg agccgaaccg gcccaggaga tcccccacca ccttcttggc gccgtcaccg
      841 acgcccacca cggagatcac ggaccccacg ccgatgacga tccccaggac cgtgagcgcg
      901 gtccgggatc ggttggtccg aatggcgagc caggcctctt ccagcagggg caccaatcgc
      961 acgggctact cctgcgtcaa ggccacgacg ggatccagcc ttgcggcctt cagcgccggg
     1021 tagatcccga agaagagtcc gatgctgatg gacactccaa gccccaccag gatggcgccc
     1081 cagggcaaga ggtagcccca gcgctgggcg atggagatca ccgcggtgat ggccatgccg
     1141 aagaggatcc cgatgccgcc accgatgagg cagatgatca ccgactcgat gaggaactgg
     1201 gccaggatgt cgcgccgctt ggcgcccagc gccttgcgga tgccgatctc ccgggtgcgc
     1261 tcgataacgg tgatgagcat gatgttcatg attccgatcc cgcccaccac cagggagata
     1321 ccggcgatca gcgtgatcac cgtggtgatg atctcgaaca cccggttggt ggtctggatg
     1381 ctgtcctccg cccgccgaac ggtgaatcgg ggcttgccgt ccaccgttcc gtaggcgtgg
     1441 tccaggtatt gggcgacctg gccggaaacg aagtccaggt ccgttacctg atgaaccttg
     1501 aagtagatcc ggttcacgcg gggaccgccg tactggtccc agttggtcat gtcggaaagc
     1561 acctcgtaag gcacgaaggc ggtatcgtca tccgagccgt cgtcgaccgg ttgcctctcc
     1621 ttcttcgtca tcaccccgat cacgtcgaac tggctgccgg ccaggctcat gctctggccc
     1681 agggccgcct cctccgtcgg gaagaatgcc agggccgtgt tcctccccag gacgactacg
     1741 cggcgccggt cgcgcacgtc cgcggcgctg acgtagcgcc ccgccgcaag gctgcgcccc
     1801 cacatccgct ggtagtccgg ccacacccca tacaccgccg ctgcctgctc atcgccgcgg
     1861 tagccgaagg agtagttgcc ccgcaggttc ggcgagaccc acgcgtcctg caccagaccc
     1921 gtgaggatgg agaggtcctc cggcttcatg agaagcacgt ccttgcccgt gggataggcg
     1981 tcccggttgg cctggaccca gaggaggctg gacccgatgt tctcgattcc cttcatgatg
     2041 gcggtgcgcc ccatgaagcc ggtggccatg accgcgatca cggaggcaac gccgatgttc
     2101 atgccaagca tcgtgaggat ggtccgaagc ttgttgtgtc gaatcatgtc cgcggattcg
     2161 cggaacccct cggtccagtt cacgttggca ctccgtccat cgcccggcgg gcgttcaccg
     2221 gctcgtctcg ggtgatcagc ccatctctca gggcgatgat cctgctggag caggcggcga
     2281 tgtccggttc atgggtgacc aggatgatgg tcaccccctg atcgttgagc tgctggaaga
     2341 tcgccatgat ctcctccccg gtgcgggagt ccaggttgcc cgtgggctcg tccgccagga
     2401 ggatggccgg ccgattgatg agcgcgcgcg cgatggccac gcgctggcgc tggccgccgg
     2461 agagctcgtt gggacggtgc agggcccggt tctccaatcc cacctgcttt agcgcggcca
     2521 tggcccgatc cctgcgctcg cgcttcgaga tcccgctgta gatcagggga agctcgacgt
     2581 tgcgcagcga gctcatccgc ggaagcaggt tgaaggtctg gaagacgaat ccgatgcgcc
     2641 gattgcggac ctcggcaaga tcgttgtctc ccatgtcgga tacgtcgatc ccatccagca
     2701 cgtagcggcc cgtagtgagc cgatccaggc agcccacgat gttcatcagg gtggacttgc
     2761 cggaccccga cggtcccatg atggccgcga actcgtgctc ctcgatgcgc aggtccacgc
     2821 cctggagcgc cggaacctcg agcttgccca gacggtagat cttgcggacg ttctccagcg
     2881 cgatcatggg cgtcagtcct gtcggacggt gaccaggatg ccctcccgga tcagcttgag
     2941 gttgccagca acgatccggt ccccttcggc gagccccttg gtgacctcga tcgagttcac
     3001 gtcgctggac ccggtcccga tctctcgccg ggcaagctgg tagatatccg cctgggcagc
     3061 gttcttcttg ccggtgggct ggagcaggta aaccgtggtc acgttctcct cggtgacgaa
     3121 actggaaagc gggaccagga ggacgttctc tttgatactg gtgacgatgc gcgcggtgca
     3181 cgacgcgccg gagcgcagcg tggccgaacc gtccgaggtc ttccgagacg cgggatcgac
     3241 cttcacgtcc accatgctca cccgggtgga gccgacgctg gtgatgaccg gcgagatggt
     3301 gtccaccgtg ccggtcaacg tcagaccgat cagcgaatcg ctcgtgatct ccgccggctg
     3361 accaacccgg atcttgccga tgtccacctc gtcgatctgg atccgcgcca gcatgtcatc
     3421 cagggtctcg atcttgacca ggggtgtcga tggggacacc tggtcgccca cggagggctt
     3481 cacgtcggtg acgactcccg ccgccggcgc cgtgatggta caccgggtca cggtgtcctg
     3541 agcggactgc agggagagga gcgcctgctc gacctccgga gaggcctcga tgatcttgtc
     3601 gtcctgggtg gcgtccagcg gcggcgtcgc gtcgagaggg agcccacagc gcagatcgag
     3661 ctgctcccgc gccgactgga ggttcacctg ggccgtgtcc acggcgtcgg aagcctgttg
     3721 gtagctctcc tgggagaccg cctttgccgc gaagagctcc ttgctcttgg agaaggtgcg
     3781 ctgcgcgtcg ctcacggcgg acgtggcgga gcgaaactgc gcgcggagcg tgaccaggga
     3841 ttggcggatg ctcttgcgcg tggcatccag ggaggcgcgc atcttctgcg ccgccaggac
     3901 gtagtcgtcg tcccgaagct tcaggagcac gtccccggcc tgaacccggt cgttctccgc
     3961 cacacggatg gaccggacct ccgctgagac ctgggacgtt accgtgaatg cgtccttggg
     4021 cacgaaggtc ccgttgccgc tcacgcgatc ctgaaggctc ccccggttca ccaggtaggc
     4081 gcggacgggg atggccgtct cgtaccggct cccgcgcaga gtgaggttga gcacgatgac
     4141 ggccgccacc agcaccagga tggtgacgat cacgaccagg acgccgcgtc tcttcatgag
     4201 tgagttcctt ccaggatggg ggccagatcc tcgccggcca tggattccag atccagcacc
     4261 gtgaggagga ggtcggcgcg ggcgcgccag aggtcgttgg cgctgaggtc cacctcagcc
     4321 tccttggcgg ccacgttgac gtccgtggac ttcccgaggc tcaggaggct ctgttcggtg
     4381 gtcagtcgcc gctgcgccag gctcagggac tgggtgagca gggtgacctt ctcgtccagg
     4441 ctg
//
LOCUS       DASRZS010000305         5117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1709311, whole genome shotgun sequence.
ACCESSION   DASRZS010000305 DASRZS010000000
VERSION     DASRZS010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709311"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFH83_07540"
     CDS             <1..591
                     /locus_tag="VFH83_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HET6486257.1"
                     /translation="EHELNASTFTARVVASTMSTCYCSISAAIGALFGFLHGGANEKV
                     LDMVEEIGSKDNVGPWLDKTLAENRKVMGMGHREYKVKDPRSSVMEEFLQTISDKKGD
                     HRNYDILKELEKQFRARMEQKGKPLYPNVDFFSGSVYTLLGIPKILFTPVFAMSRVAG
                     WLAHILEQREDNRIYRPESLYRGSVERHYEPLARRS"
     gene            complement(564..1328)
                     /locus_tag="VFH83_07545"
     CDS             complement(564..1328)
                     /locus_tag="VFH83_07545"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6486258.1"
                     /translation="MNQRRRPSPGTWRAFRISFSIPLSLPQPRSGPVAESPPHTTIYF
                     HEYTQSREEKQRMTLDDRLALLGDEVTGGMWADLGAGAGAFTLSLAERIGPGGRIYAV
                     DRDERALRELRDRLSGMERGADPIGPEIETVAADFSEALPLRDLDGILLANSLHYQRD
                     ACAVLRRLRRLVRPGGKVLVVEYDVRRRSPWVPFPLPAALFGTVAECAGLVRPRLIAE
                     RPSAYHGRAYSAAAEVPRAGESPDSDRDQLRRARGS"
     gene            1267..1629
                     /locus_tag="VFH83_07550"
     CDS             1267..1629
                     /locus_tag="VFH83_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486259.1"
                     /translation="MLNEIRNARQVPGEGLRRWFTDDYFDLIAWYDEDGTFFGFQLCY
                     DKQSRERALTWTRDHGFRHDAVDPGETPGQAKMSPVIVADGEFRPQPVARRFRQESGN
                     MDPQLALLVYDMILSYRA"
     gene            complement(1678..2397)
                     /locus_tag="VFH83_07555"
     CDS             complement(1678..2397)
                     /locus_tag="VFH83_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET6486260.1"
                     /translation="MKGKKKDLPGPRGGFRALVIAAHPDDPEFGCAGTVARWAAQGKH
                     VTYLLLTSGDKGSKDPSRRPGDLAALREREQTAAAARLGVTDVIFLRHPDGLLENSMA
                     MRREVVGVIRRIRPHAVLAIDPWRHYQTHPDHRAAGEAALDAVYAAKEVNLFPEQLVG
                     DIQPWRVKEVYLFWTDSPDYWEDITTSIPQRIAALQSHTSQVGRKRAGIEKNIRTNAH
                     ETARKANLSVEYAEAFKLLRF"
     gene            2517..3614
                     /locus_tag="VFH83_07560"
     CDS             2517..3614
                     /locus_tag="VFH83_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6486261.1"
                     /translation="MTSRERMLTALSNGRPDRLPCQVHDWMDYYLRTYLPGLDRWQAY
                     EKFGMDIAMYVEPVYRYSDADRSRWRVRRREWPGEEGRTLFEETIETPKGDLRRAGAS
                     NQFTTWETEVLVKTPDDFELWNEFLPMPIGVDFTAIQAAKDRLGDRGIIRSHPFSPGQ
                     GSPWQSFCVMVGTEPAIMMAMDTPRFVHHALEAILQKTLRVTEMWKGIPSDMVETGGG
                     AGSNTVISPTMFQEFCVPYDRRQHALLHDAGVKIVYHLCGGVMKMLDLVTQNGADGLE
                     TMTPPSMGGDCDLAEASRRVGDRLFFMGGFDQNAGFERGTPQRARQLVFECFEATKDH
                     AGYICVPSDHFFFGDPANLQAFADACKECRY"
     gene            complement(3665..3907)
                     /locus_tag="VFH83_07565"
     CDS             complement(3665..3907)
                     /locus_tag="VFH83_07565"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET6486262.1"
                     /translation="MQGSERVQTEIQRVIGDDPTISEANRIIVSVEKKGFWLTAKECV
                     LLKGHVHADTDRVKAEKIATLHSGGRQVINEISVLH"
     gene            complement(4001..>5117)
                     /locus_tag="VFH83_07570"
     CDS             complement(4001..>5117)
                     /locus_tag="VFH83_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486263.1"
                     /translation="ATGAVAPTGAMPPFAFGNDPSKVRGYREFWARSERRRPLVGFSI
                     KSWFPLEEFAASRAWKGSRVLTPDLVDPPAFLDDQERLLREGETMADDILRGASPSQA
                     VPWLAGMLGSVLRILPGSILEEAQHLPWEQVLAKRLDPGEPWFRAYHECCRTLADRAA
                     GRFPVSHGSLVGPTDLLADFRGHTEALFDLVDEPDRCAQALWGFGRIFRELTVATWDR
                     LPRFQGGWFDAQYQLWTPGPTIRMQEDAAAAYSPELYRRLVQPVDRYLAESFSCAFMH
                     LHSTSLAVLDAILEIEELGCIQMNYEVGSGGPDMAGMIPPFRRIQEAGRSLVVRGSFT
                     PEEARLLADSLDPRGLYVYAMVESLQEADALRPILGM"
BASE COUNT          888 a         1707 c         1683 g          839 t
ORIGIN      
        1 gagcatgagc tgaacgcctc cacgttcacg gcccgggtcg tggcctccac gatgtccacc
       61 tgctactgca gcatctcggc tgcaattggc gcgctattcg ggttcctgca cggaggcgcc
      121 aacgagaagg tgctggacat ggtcgaagag atcgggtcca aggacaacgt cggaccgtgg
      181 ctggacaaga ccctggccga gaaccgaaag gtcatgggga tgggtcatcg ggagtacaag
      241 gtcaaggatc cccgctcctc ggtgatggag gagttcctcc agaccatctc ggacaagaag
      301 ggggaccacc gcaactacga catcctcaag gagctggaga agcagttccg ggcccgcatg
      361 gagcagaagg ggaagccgct gtatccgaac gtggacttct tctccgggtc cgtctacacc
      421 cttctcggga tcccgaagat cctctttacg ccggtgttcg ccatgtcacg ggtggccggc
      481 tggctggccc atatcctgga gcagcgtgag gacaaccgga tctaccggcc cgagagcctc
      541 taccgaggat ccgtcgagcg ccactacgag cctctggctc ggcggagctg atctcggtcg
      601 gaatcgggcg actccccggc acgcgggacc tccgccgccg ccgagtaggc gcgcccgtgg
      661 tacgcgctgg gccgctcggc gatgaggcgc ggccgcacca ggccggcgca ttcggcgacg
      721 gtcccgaaga gagcggcggg caacggaaac gggacccagg ggctgcggcg ccgaacgtcg
      781 tactcgacca ccaggacctt ccctccgggc ctcacgagtc ggcggagccg cctaagcacc
      841 gcgcaggcgt ctcgctggta gtgcagggaa ttggcgagca ggatcccatc gagatcccga
      901 agggggagag cctcggagaa gtcggccgcc accgtctcga tctccggtcc gatcggatcg
      961 gcgccccgct ccattccgga cagccgatct cgcagctccc gcagcgcgcg ctcgtcccga
     1021 tccacggcgt agatccggcc tcccggcccg atccgctccg ccagcgagag cgtgaaggcg
     1081 cccgccccgg cgccaaggtc cgcccacata ccgcccgtga cctcatcccc cagcagggca
     1141 agccggtcat ccagggtcat ccgctgtttt tcctcacggg actgagtata ttcatggaaa
     1201 tagatagttg tatgcggtgg gctctccgcc acggggcccg accgcggctg tgggagggat
     1261 agtggtatgc tgaacgagat ccgaaacgcg cgccaggtcc cgggggaggg cctgcgccgc
     1321 tggttcaccg acgactattt cgatctgatc gcatggtacg acgaggatgg aacgtttttc
     1381 ggattccagc tctgctatga caagcagtcc cgcgaacggg cgctcacctg gacccgagat
     1441 cacgggttcc gccacgacgc cgtggacccg ggggagaccc ccggtcaggc caagatgtct
     1501 ccggtcatcg tggcggacgg ggagttccgc ccccagccgg tggcccgcag gttccgccag
     1561 gagagcggca acatggatcc gcagctcgcc ctcctggtgt acgacatgat cctgagctac
     1621 cgcgcctgac ccggtcccca gcccgcgcgg accgatctcc ctcggtggcg acgggcgcta
     1681 gaacctcaac agcttgaatg cctccgcgta ctcgacggag aggttggcct tacgggccgt
     1741 ttcatgcgcg ttggtccgga tgttcttctc gatccccgcg cgcttccgcc caacctggct
     1801 ggtatggctt tgcagcgcgg cgatcctttg cgggatgctg gtggtgatgt cctcccagta
     1861 gtcgggggaa tcggtccaga acagatagac ctccttgacc cgccagggtt ggatgtcccc
     1921 cacaagctgc tccgggaaca ggttcacttc cttggccgcg tagacggcgt ccagcgccgc
     1981 ctcgcccgcc gcgcggtggt cggggtgcgt ctggtagtgc ctccacggat cgatggcgag
     2041 cacggcgtgg ggacggatgc ggcggatcac gccgaccact tcccgccgca tggccatgga
     2101 gttctccagg aggccgtcgg gatggcggag aaagatcacg tccgtcacgc ccagccgggc
     2161 cgccgccgcc gtctgctcgc gctcccgcag cgcggccaga tcgcccggac ggcgagacgg
     2221 atccttgctc cccttgtctc cgctggtcag gagaaggtac gtgacgtgct tcccctgggc
     2281 ggcccaccgg gccaccgtcc cggcgcagcc gaactcgggg tcatcggggt gagcggcaat
     2341 cacgagcgca cggaagccgc ctcgtggacc aggcagatcc tttttcttgc ctttcatgtc
     2401 gggaaccgta gcatgaccgc gccgcgctgg gccatggggt gcggggggag cgccagtccg
     2461 cccttgatcc tctccgcccc actccctatc ctgaggccac atccaggggg tactccatga
     2521 ccagccgtga acgcatgctc accgcgcttt ccaacggaag gccggaccgg cttccctgcc
     2581 aggtccacga ttggatggac tactatctgc gcacctatct gcccggtctg gaccgctggc
     2641 aggcgtacga gaagttcggg atggacatcg cgatgtacgt tgagcccgtg taccgctact
     2701 cggacgcgga ccgctcccgg tggcgggtgc gccgccgcga atggcccggt gaggagggcc
     2761 gcaccctgtt cgaggagacc atcgagacgc cgaagggcga cctgcgtcgc gcgggcgcct
     2821 ccaaccagtt cacgacgtgg gaaaccgagg tgctggtcaa gacgccggac gatttcgagc
     2881 tttggaacga gttcctcccc atgccgatcg gcgtcgactt cacggccatc caggcagcca
     2941 aggaccgact gggggatcgc gggatcatcc gcagccaccc gttcagcccg ggccagggca
     3001 gcccgtggca gagtttctgc gtcatggtgg ggaccgagcc cgccatcatg atggccatgg
     3061 acactccgcg gttcgtccac cacgcgctgg aggccatcct ccagaagacc ctccgcgtga
     3121 ccgagatgtg gaagggcatc ccctcagaca tggtggagac cggcggcggc gccggctcca
     3181 acacggtgat cagtccgacg atgttccagg agttctgcgt gccctacgat cgccgccagc
     3241 acgcgctcct ccatgacgcc ggggtgaaaa tcgtctacca cctgtgcggc ggcgtgatga
     3301 agatgctgga cctggtgact cagaacgggg cggatggtct ggagaccatg accccgccct
     3361 ccatgggcgg cgactgcgat cttgccgagg cgtcccgccg agtcggggat cgactcttct
     3421 tcatgggagg cttcgatcag aacgccggct tcgagcgcgg gacgcctcaa cgcgcacgcc
     3481 aactggtctt cgagtgcttc gaggccacga aagaccacgc ggggtacatc tgcgttccct
     3541 cggaccactt cttctttggg gatccggcaa accttcaggc gttcgcggat gcgtgcaagg
     3601 agtgccggta ctgagggaat gccacggacg gcgcggcgta ccgcgccggg ccccgcggcc
     3661 ggcactagtg caagacggat atctcgttga tcacctgcct gcctccgctg tggagggtgg
     3721 cgatcttctc cgccttgact cgatccgtat ccgcgtgcac gtggcccttg aggaggacgc
     3781 attccttggc cgtcagccag aagcccttct tctccacgct cacgatgatc cggttcgcct
     3841 cggaaatggt gggatcgtcg ccgatcactc tttgaatctc ggtctggacc cgttcgcttc
     3901 cctgcatatc gcgcctcctt cgtaccagcg ccacggcagg acgatcaaac cctgacaagc
     3961 gaattatacg cccactccgg cgggctgtca aaccaaaacc ctacattccc aggatcggac
     4021 gaagcgcgtc ggcctcctgg aggctttcca ccatcgcgta gacgtacaga ccgcgtgggt
     4081 ccagagagtc cgccaggaga cgggcctcct ccggggtgaa ggagcctcgg accaccaggg
     4141 accgaccggc ctcctggatt ctgcgaaacg gcgggatcat gccggccatg tccggtcccc
     4201 cggatcccac ctcgtagttc atctggatgc agccaagctc ctcgatctcg aggatggcat
     4261 ccagaacggc aagggaggtg gagtggaggt gcatgaaggc gcaggaaaag ctctccgcga
     4321 ggtaccggtc gacgggctgg accaggcgac ggtagagttc gggggagtac gcggcagccg
     4381 cgtcttcctg catgcggatg gtcggacccg gcgtccagag ctggtattgc gcatcgaacc
     4441 aaccgccctg gaaccggggc aaacggtccc aggtggcaac ggtcagctcg cgaaagatcc
     4501 ggccgaagcc ccagagcgcc tgcgcgcagc gatctggctc atccaccagg tcgaacaggg
     4561 cctcggtgtg acctcggaaa tcggccagaa ggtccgtggg tcccaccagg gagccgtggc
     4621 tgaccggaaa ccgacccgcg gcgcgatccg ccagggtacg acagcactcg tggtaggcgc
     4681 ggaaccacgg ctcgccgggg tccagacgct tggccagcac ctgctcccag ggcagatgct
     4741 gcgcctcctc caggatgctg cctggcagga tgcgcaagac actgccgagc atcccggcca
     4801 gccacgggac ggcctgggac ggagaggcgc cgcggaggat gtcatccgcc atggtctccc
     4861 cttccctcag caggcgctcc tggtcgtcga ggaacgcggg aggatccacg aggtccggcg
     4921 tgaggactcg gctccccttc cacgcccggc tggccgcgaa ctcctcgagg gggaaccagc
     4981 tcttgatgga gaagcctacg agagggcggc gccgctcgga tcgggcccag aactcacggt
     5041 aacctcggac cttggacgga tcgttgccga acgcgaatgg cggcatggcg ccggtcggcg
     5101 ctacagcgcc ggtggct
//
LOCUS       DASRZS010000306         2448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2093324, whole genome shotgun sequence.
ACCESSION   DASRZS010000306 DASRZS010000000
VERSION     DASRZS010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093324"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFH83_07575"
     CDS             <1..604
                     /locus_tag="VFH83_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486264.1"
                     /translation="KTYLWGGLYEINHEYSPMEALDGVENSPSEHYFKFDPQGFAYDP
                     DRAAFLARLAALRTGPANRYLAYGVMLRPPDVQPSDTRMSWFHYNSNQKTREYRDRGS
                     IVVPGIIASAWQQGTGPSLSFAFLFANTEGKEVTVQATVDRKRYGITGDGWKMRILSG
                     FDKGRTATSRETGPLGADPTVALSLPLASRDLLMVEFHRE"
     gene            633..1919
                     /locus_tag="VFH83_07580"
     CDS             633..1919
                     /locus_tag="VFH83_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6486265.1"
                     /translation="MKRRMAALFSGLVLLQGLAFAAPKTTVVLWNRIFEDWNRAYCER
                     MAAAYNAQSTTVTLDQQFIPGDGWDEKMKAAQASGTAPDIYLMNYGGIPWLARDGMLL
                     RMDNLLPAAAWNDLYDNVRKFISYKGGFYAYPQLLEPATVLYYRKDLFQQAGLDPNKP
                     PRTWDELIADARKLTTKQVFGLAVPGFGGDVGWSTWGWQQTASGHMAISDDWSKATVQ
                     DQGYLDLANFFKRVYAEKVVPAQPLAGYADITPFAQGGLAMAFCGSWAIGEIRNNYKD
                     MVDKIGVAAAPTKDGKPFTPTVGGWTYVLDAKTKVAPSAADYINWLLASDPSRPAEFF
                     SVAKYSKFSPRKSVDAFIKKSNEAMNDPWMKTISEQVVPYTVAEPLYAWDISMAVDTA
                     LQSVVVSNATPADALSKAADSINNYIQSNSYSTKKP"
     gene            2000..>2448
                     /locus_tag="VFH83_07585"
     CDS             2000..>2448
                     /locus_tag="VFH83_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6486266.1"
                     /translation="MDHATAYLMILPALAFLTVFVFVPLVMAVQKSFSDWNFYSASVF
                     VGLRNFGLVLRNELFLTSLVNGFKFVGIIVPSQLVLSFLFAHVLKGTRGSLSSVVKTA
                     IYVPAVISGIIASVIFLFLLNYRAGLRNQALSWFGIDRVAFLTVPWLA"
BASE COUNT          437 a          794 c          790 g          427 t
ORIGIN      
        1 aaagacctac ctctggggcg gcctctacga gatcaaccac gagtacagcc ctatggaggc
       61 gctggatggc gtggagaaca gcccctcgga gcactacttc aagttcgacc cgcagggttt
      121 tgcctatgat ccggatcgtg ccgccttcct ggcccggttg gctgccctga ggaccggccc
      181 cgccaaccgg tacctggcct acggcgtgat gcttcggccg ccggacgtcc aaccctcgga
      241 cacgcggatg tcctggttcc actacaacag caaccagaag acccgcgagt accgggaccg
      301 cggatccatc gtggtgccgg ggatcatcgc cagcgcgtgg cagcagggga ccggaccgtc
      361 gctctccttc gcgttcctct tcgccaatac cgagggaaag gaggtgaccg tccaggccac
      421 cgtcgaccgg aagcgatacg ggatcaccgg ggacggctgg aagatgcgga tcctgtccgg
      481 gttcgacaag ggccgtacgg cgaccagccg tgagacgggc cctctgggcg cggaccccac
      541 cgtggcgctg agcctgccgc tggcgtctcg ggatctcctc atggtcgagt tccatcgaga
      601 atgagcacgg aaaggaagga aaggagatgc ttatgaagcg taggatggcg gccctgttca
      661 gcggactggt gctgcttcag ggtcttgcgt tcgcggcgcc caagaccacg gtggtcctgt
      721 ggaaccggat cttcgaggac tggaaccggg cgtactgcga gagaatggcg gcggcgtaca
      781 acgcccagag caccacggtc accctggacc agcagttcat ccccggcgat ggctgggacg
      841 agaagatgaa ggcggcccag gcctccggga cggcgccgga catctacctc atgaactacg
      901 gcgggatccc ctggctggcc cgggacggga tgctgttgcg gatggacaac ctcttgcccg
      961 cggccgcctg gaacgatctg tacgacaacg tgaggaagtt catctcgtac aagggcggct
     1021 tctatgccta tccgcagctc ctggagcccg ccacggtcct ctactatcgc aaggacctgt
     1081 tccagcaggc ggggctggac cccaacaagc ctccgcggac ctgggatgag ctgatcgcgg
     1141 acgccaggaa gctcaccacc aagcaggtgt tcgggctggc ggtgccgggt tttggtgggg
     1201 acgtgggatg gtccacctgg ggctggcagc agaccgcctc cgggcacatg gccatcagtg
     1261 atgactggag caaggccacc gtccaggacc agggctacct ggacctcgcc aacttcttca
     1321 agcgcgtcta cgcggagaag gtggtccccg cccagcccct ggcaggttac gcggacatca
     1381 cgcccttcgc gcagggcggt ctggccatgg cgttctgcgg gtcctgggcc atcggggaga
     1441 tccgcaacaa ctacaaggac atggtggaca agatcggtgt cgcggcggca cccaccaagg
     1501 acggcaagcc gttcaccccg acggtgggag gctggaccta cgtgctggac gccaagacca
     1561 aggtggcgcc ctccgccgcg gactacatca actggctgct tgccagcgac ccgtcgcggc
     1621 cggccgagtt cttctccgtg gccaagtact ccaagttctc gccgcggaag tccgtggacg
     1681 cgttcatcaa gaagagcaac gaggcgatga acgacccctg gatgaagacc atctccgagc
     1741 aggtggtgcc gtacaccgtg gccgagccgt tgtacgcctg ggacatcagc atggcggtgg
     1801 acacggcgct gcagagcgtg gtggtgagca acgccacccc ggcggatgcg ctttccaagg
     1861 ccgcggactc catcaacaac tacatccaga gcaacagcta cagcaccaag aagccttgag
     1921 gacggatcgt ttccggatcc cgggggcccc gcgcggggcc cccgcaaggc ggcgtccccc
     1981 ccgcctgccc acccgccgga tggaccacgc cacggcgtac ctgatgatcc tgccggcgct
     2041 cgccttcctg accgtgttcg tctttgtgcc cctggtcatg gcggtgcaga agagcttcag
     2101 cgactggaac ttctattccg ccagcgtctt tgtggggctt cgcaacttcg gcctggtatt
     2161 gaggaacgag ctcttcctga cctccctggt gaacgggttc aagttcgtgg ggatcatcgt
     2221 tccttcccag ctcgtgctct cgtttctctt cgctcacgtg ctcaagggta cccgcgggag
     2281 cctgtcctcc gtggtgaaga ccgccatcta cgtcccggcg gtgatctccg gcatcatcgc
     2341 ctccgtcatc ttcctctttc tgctgaacta ccgggccggt ctgaggaacc aggcgctgag
     2401 ctggttcggg atcgaccgcg tggcgttcct gacggtaccg tggctggc
//
LOCUS       DASRZS010000307         7496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1709553, whole genome shotgun sequence.
ACCESSION   DASRZS010000307 DASRZS010000000
VERSION     DASRZS010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709553"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFH83_07590"
     CDS             complement(<1..450)
                     /locus_tag="VFH83_07590"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET6486267.1"
                     /translation="MNAIPARKYARIFSFAARDQAVYLPAFLVRNLFFVVIVFVFWSL
                     WRAVYSGRDALGGLSLAQMIWYLTFTETIELSKSRVLYQVQEEVKDGTLAVTLTRPYS
                     YVLSHLSRAMGESLVKLCPLLVEGGLLATLMVGPLPGYLRALPMGLVL"
     gene            complement(447..1442)
                     /locus_tag="VFH83_07595"
     CDS             complement(447..1442)
                     /locus_tag="VFH83_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6486268.1"
                     /translation="METVLSVQHLTKVFTTRTKPEDRGRPAGAFRSFFSPAHRTVRAV
                     DDVSFEVRRGELLAFIGPNGAGKSTTIKCITGILYPSAGDVTVLGMNPHRQRRRLAYR
                     IGTVFGQKSQLWFHLPPLDSLRLLGDIYDLDRKEAERRIAFLTETFEIGPYLRTPVRR
                     LSLGERIRCEIAASLLHEPEILFLDEPTIGLDVVVRQTIRDLILRINAERRTTIFLTS
                     HDAGDIEKICRRVIVINHGRLVWDDTVKTMKYAYLRKKVIDLKLSAPLSLTLPGVRVL
                     KAKDYSAKLEVDLTKVGLDDVVSRLLRRNKVEDITISNPPMEQIIAMIYGREEGP"
     gene            1631..2674
                     /locus_tag="VFH83_07600"
     CDS             1631..2674
                     /locus_tag="VFH83_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486269.1"
                     /translation="MERFATIPKSTRIRGIQWTADRIPYPEPDVKGDTFPMTWADDGE
                     IYASAGDPLWGETPSGLDVEKLSGEPTGYKITKVNPMNDYLGWGGDGAKPSGMICVDG
                     ILYLAFQNLLRAKKPPFSLRSQHGSDAHIVYSTNRGMIWVPAFGTIKSPMFPGHRFGG
                     PAFVNYGQNNANARDGYVYAVSSDQWDNGSNLRVGRVPLDSIVRREAWEWVCAFTPAA
                     EPAWSHDLEEAVPVLSIHRWISIPEMVYLAGIQRYLLVLWRLHADFSPDDGTDLMILE
                     SPEPWGPFSLVHFEEYWEGKEYNPYCPRIPLKWMQSDGVSGWLQFSGSWGPRAQKAGY
                     YRSNVRPFRLLMA"
     gene            complement(2664..3470)
                     /locus_tag="VFH83_07605"
     CDS             complement(2664..3470)
                     /locus_tag="VFH83_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HET6486270.1"
                     /translation="MEFRRYVPAEERRRNAQAEQARMRRRGQDVQPIHIEGREIARTF
                     WGKAWCQHLESFSDFENRLPRGRTYVRNGSVCHLEIGAGAVRALVQGSTLYEVTIGVA
                     PLGARAWGAIKEKCTGQISSLLDLLQGRLSSGVMAIVSDRDQGLFPKPREISLDCSCP
                     DWASMCKHVAAALYGVGARLDEKPELLFLLRGVDHQELITSHAEAAVQATVTQGKARR
                     IAEADLAEIFGLELDPALAQAPAAPRRPSKKRGRKRKPSPKKRGRKRPKP"
     gene            complement(3477..6164)
                     /locus_tag="VFH83_07610"
     CDS             complement(3477..6164)
                     /locus_tag="VFH83_07610"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6486271.1"
                     /translation="MGDEPPLDGLLSITPGGHLMVASAVADRPGMAPGLRRVLEAFRV
                     SPAEGLFELAARPISGGLPPELAYWREFAALYLGALCHTPAGTSQPEPIPPPGPDELL
                     ARVLSVPPTQGVEYLSADVLVGLWSSLDEWVRGQASAAGSLETFLTAHAPLWRQVGRV
                     CFHLAENKKDAEYPFAFLATYAPRLTESARIRYQPLGRALQELAGERNRKALVHLLTP
                     VQRAAEKSHLIRALVDSSDIYQPLAWTPDDAYAFLKDVPLYEEAGVLVRVPDWWTRRS
                     RPQVAVKIGSQAGSVLGADSLLDFTVETVIGDSALSEAEVRKLLAGADGLVFLKGRWV
                     EIDRARLKEALDHWKRMERRVEEGGISFVEGMRLLAGAPPDLEDAGDAATAGWSFVEA
                     GAWLKDILARLRAPQADGGPQRPGLSATLRPYQEAGVTWLRLLTGLGLGACLADDMGL
                     GKTMQVLALLLALRKEQPGPSLLVLPASLLANWKSEIDRFAPDLRAAYLHPSMQEPQT
                     EAADLIRRAGEFDLVVTSYGMLSRVPDLAAVAWNLVVLDEAQAIKNPGTRQTRLAKRL
                     RARARIALTGTPVENRLSDLWSIFDFLCPGLLGSQARFKAFVEILEKRQGERYAPLRA
                     LVRPYILRRLKTDRTVIADLPEKTVVSAWCGLSRAQAALYQRVVNELAKEIHTRDGMR
                     RRGLILSTLLRLKQICNHPAQLKGDGAWDPEESGKFLRLGELAEEIASRQDRALVFTQ
                     FREMTEPLASFLASVFGRPGLVLHGQTPVKERQNLVQAFQAENGPPFFVLSLKAGGTG
                     LNLTAATHVIHFDRWWNPAVENQATDRAFRIGQKRNVLVHTFVCRGTVEEKIDALIQE
                     KTALATDVLEGGGEALLTEMTDDELLSTVALDIGRARSE"
     gene            complement(6192..>7496)
                     /locus_tag="VFH83_07615"
     CDS             complement(6192..>7496)
                     /locus_tag="VFH83_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486272.1"
                     /translation="LFAEANPPHWIRIAGLGRGSEGYKTVRVGEVEEVGNRENDGHGI
                     CMWGRYMMWHWKGRPAEWNRRRFAATRAAVDWIQWQLDTDHLRPGVRKDVLYTESECA
                     HGDYDIYSTYNCLHGAKLSARMARALGEGALADQWEALVRRLRQGILDHLVDPSPVGP
                     IWHTEDVCDWQDHAHKLVHLHLSTEGDSYTPLQDYGAGDDLERRCLEISRNSYRYLMG
                     RGAGGTEGAASASTFNCLRMYGYGQGMMTQAALLLDEMTDAGRFLDLLLRYCYLPRLH
                     GWASPEGIITHRSMKYYLPVNGYMGQDSHVADSTKAVRLILGIDDNDPDHLRLVPRYP
                     SDWTRMAISEYPVLTGSARQRLAYVYERDGGKHTLRYDLERPTDRLSVRFGPFPRGTV
                     VHAASVNGKPAAARSLDSGDSAWAWVSIGNAGRTGDVTIPVT"
BASE COUNT         1267 a         2504 c         2513 g         1212 t
ORIGIN      
        1 caggaccagc cccatgggca gcgcccgcag gtatccgggc agcgggccca ccatcagggt
       61 ggccaggaga ccgccctcga ccaggagcgg gcagagcttc accaggctct cccccatggc
      121 tcgggagagg tgggagagca cgtacgagta gggacgcgtc agcgtcaccg ccagggttcc
      181 gtccttgacc tcctcctgga cctggtacaa cacccggctc ttggagagct cgatggtctc
      241 cgtgaacgtc agataccaga tcatctgcgc caggctgagc ccgcccagcg cgtcgcgccc
      301 ggagtagacc gcgcgccaga gggaccagaa cacgaagacg atcaccacga agaagaggtt
      361 gcgcaccagg aacgcgggca ggtagaccgc ttgatcccgc gccgcgaacg agaagatgcg
      421 cgcgtacttg cgggcgggga tggcgttcat ggtccctcct ctcgcccata gatcatggcg
      481 atgatctgct ccatcggcgg gttggagatc gtgatgtcct ccaccttgtt gcggcgcagg
      541 aggcgggaga ccacatcgtc cagccccacc ttggtgagat cgacctccag cttggcggag
      601 taatccttgg ccttcaggac gcggacgccg ggcagggtca gggacagcgg ggcgctcagc
      661 ttcaggtcga tgaccttctt ccgaagatag gcgtacttca tggtcttcac ggtgtcgtcc
      721 cacaccagtc ggccgtggtt gatcacgatg acccggcggc agatcttctc gatgtccccg
      781 gcgtcatggg acgtgaggaa gatggtcgtc ctgcgttcgg cgttgatccg caggatcagg
      841 tcccggatgg tctggcgcac caccacgtcc aggccgatgg taggctcatc cagaaaaagg
      901 atctcgggct cgtgcaggag ggaggcggca atctcacatc ggatccgctc gcccaaggag
      961 agcctgcgga ccggggtcct gaggtaggga ccgatctcga aggtctccgt gagaaaggcg
     1021 atccgccgtt cggcctcctt ccggtccagg tcatagatgt ccccgagaag gcgcagcgag
     1081 tcgaggggcg gcaggtggaa ccagagctgc gacttctgcc cgaacacggt gccgatccgg
     1141 taggccagtc gcctccgctg gcgatggggg ttcatgccca gaacggtgac gtccccggcc
     1201 gacgggtaaa ggatgcccgt gatgcatttg atggtggtgg acttgccggc cccgttcggg
     1261 ccgatgaagg caaggagctc tcctcgccgc acctcgaagg aaacgtcgtc caccgcgcgc
     1321 acggtacggt gggccggcga gaagaagctg cggaaggccc ccgcgggacg gccgcggtcc
     1381 tcaggcttgg tgcgggttgt gaacaccttg gtgagatgct ggacggaaag gactgtttcc
     1441 atggcacatt ctatagaaga acgcggggcc gcgcaaccga gcgcttgccc gcctcggatg
     1501 ggtccatgcg caccggatag cctccactct ccttgcgcgc caggctgatg aaccgcgaca
     1561 aggctctgct gcgggttgcg aaggccacgc ggatcgcgtc atagtggtgg tcatccaagg
     1621 gggacaacca atggaacgct ttgccacgat tcccaagagc acccggatcc gcggcatcca
     1681 gtggaccgcg gatcgcatcc cgtacccgga gcccgacgtc aagggcgaca ccttccccat
     1741 gacctgggcc gacgatgggg agatctacgc ctcggcgggc gacccgctgt ggggcgagac
     1801 gcccagcgga ctggacgtgg agaagctgag cggggagccc accggctaca agatcaccaa
     1861 ggtcaacccc atgaacgact acctgggctg gggcggagac ggggccaagc ccagcggaat
     1921 gatctgcgtc gacgggatcc tctacctggc gttccagaac ctgcttcgag caaagaaacc
     1981 gccgttcagc ctgcggtccc agcacggcag tgacgcgcac atcgtctatt ccacgaaccg
     2041 cggcatgatc tgggtgccgg ccttcggcac catcaagagt cccatgttcc cgggccatcg
     2101 cttcggcggg ccggcgttcg tcaactacgg gcagaataac gcgaacgccc gagacggcta
     2161 cgtctatgcg gtctcctccg accagtggga caacgggagc aacctccgcg tgggccgggt
     2221 gcctctggac agcatcgtcc gcagggaggc ctgggagtgg gtgtgcgcgt tcactccggc
     2281 cgccgagcct gcctggagcc atgacctgga ggaggccgtt cccgtgctga gcatccatcg
     2341 ctggatcagc attccggaga tggtctacct cgccggtatc caaaggtacc tgctggtgct
     2401 ctggcggctg catgcggact tctcgccgga cgacggcaca gacctgatga tcctggaatc
     2461 tcccgagccg tggggtccgt tctctctggt ccacttcgaa gagtactggg agggcaagga
     2521 gtacaacccg tactgcccgc gcatcccgct caagtggatg cagagcgatg gggtcagcgg
     2581 ctggctgcag ttctccggaa gctggggacc gcgggcgcag aaggcgggct actaccgctc
     2641 gaacgtgcgc ccgttccgcc tgctcatggc ttaggacgct ttcggccgcg ctttttcggg
     2701 ctcggtttcc gctttcggcc gcgcttcttc gagggccgcc gcggagccgc tggagcttgc
     2761 gccagcgccg gatccagctc caacccgaag atctccgcga ggtccgcctc ggcgatcctg
     2821 cgtgcctttc cctgggtgac ggtggcctgg accgccgcct cggcgtgact ggtgatcagc
     2881 tcctggtgat ccacgccccg aagcaggaag agcagctcgg gcttctcgtc caggcgagcg
     2941 ccaaccccgt acagcgccgc ggcaacgtgc ttgcacatgg aggcccagtc cgggcaggag
     3001 cagtccaggc tgatctcgcg cggcttcgga aagagcccct ggtcgcggtc actgacgatc
     3061 gccatcaccc cagaggagag ccgtccctgc aggagatcca gcagggagct gatctggccg
     3121 gtgcacttct ccttgatcgc gccccaggcc cgcgcgccga ggggggcgac gccgatggtg
     3181 acctcgtaca gggtactgcc ttgcaccagc gcgcgcacgg ccccggcacc gatctccagg
     3241 tggcagaccg agccgttccg cacgtacgtg cggccccgag ggagccgatt ctcgaagtcg
     3301 ctgaacgact ccaggtgctg gcaccatgcc ttgccccaga acgtcctggc gatctcccgt
     3361 ccctcgatgt ggatcggttg gacatcctgt ccccggcggc gcattcgggc ctgttcggcc
     3421 tgggcgttcc tgcgtctctc ctctgcgggg acgtaccgac gaaactccac ggtccgctac
     3481 tccgatcggg cgcgcccgat gtccagcgcc accgtggaca gaagctcgtc gtccgtcatc
     3541 tccgtaagga gcgcctcccc gccaccctcc aggacgtcgg tggcaagggc ggtcttctcc
     3601 tggatgaggg catcgatctt ctcttccacg gtgccgcggc agacgaaggt atggaccagc
     3661 acgttcctct tctggccgat gcggaacgcg cggtccgttg cctggttctc cacggccggg
     3721 ttccaccacc ggtcgaagtg gatgacgtgc gtggcggccg tcaggttgag ccccgtgcct
     3781 ccggccttga gagagagcac gaagaacggc ggaccgttct ccgcctggaa cgcctggacc
     3841 aggttctgcc gctccttcac cggcgtctgt ccgtgaagca cgagtcccgg tcggccaaag
     3901 acggaggcca gaaacgatgc cagcggctcc gtcatctccc gaaactgcgt gaaaaccagg
     3961 gcacggtcct gtcgggaggc gatctcctcc gccagctcgc cgaggcgaag gaacttgccg
     4021 ctttcctcgg gatcccatgc cccatcgccc ttgagctgag ccgggtggtt gcagatctgc
     4081 ttgagccgca gcagcgtgga gaggatgagc cctcgacgcc tcatcccgtc ccgcgtgtgg
     4141 atctccttgg caagctcgtt gaccaccctc tggtacagcg ccgcctgcgc ccgagagagg
     4201 ccgcaccagg cggacacaac ggtcttctcc ggcaggtcgg cgatgacggt gcgatcggtc
     4261 ttcagccttc tgaggatgta cggacggacc agtgcccgca ggggcgcgta ccgctcccct
     4321 tgccgcttct ccaggatttc gacaaaagct ttgaaacggg cttgagagcc caggaggccc
     4381 gggcagagga aatcgaagat cgaccacaga tcggagagcc ggttctccac cggcgttccc
     4441 gtgagtgcga tccgagcgcg ggcgcgcagc cgcttggcca gccgggtctg ccgggtgccg
     4501 gggttcttga tggcctgagc ctcgtccagc acgaccaggt tccaggcaac ggcggccagg
     4561 tccgggacgc gggagagcat cccgtaggag gtgacgacga ggtcgaactc accggcccgg
     4621 cggatcaggt ccgccgcttc ggtctgcggc tcctgcatcg aggggtgcag gtacgccgcg
     4681 cgaaggtccg gggcaaaccg gtcgatctca gacttccagt tggccaggag ggaggccggc
     4741 aggaccagga gcgacgggcc cggctgctcc ttgcgaagcg ccaggagcag cgcgagcacc
     4801 tgcatggtct tgcccaaacc catgtcatcc gccagacagg ccccgagccc caggccggtc
     4861 agcaggcgca gccaggtgac acccgcctct tggtacgggc ggagcgtggc gctgaggccc
     4921 ggccgctggg gcccgccgtc cgcctgggga gcgcgcagac gcgccaggat gtccttcagc
     4981 caggcgccgg cttccacgaa agaccagccg gccgtcgcgg catccccggc gtcttccagg
     5041 tccggtggcg cgccggccag cagccgcatc ccctccacga aggagatgcc gccctcctcg
     5101 acccgcctct ccatgcgttt ccagtgatcg agggcttcct tgagccgggc tcgatcgatc
     5161 tccacccatc ggcccttgag aaagaccagg ccgtccgcgc cggcgagcag cttccgcacc
     5221 tcggcctcgc tgagcgccga gtccccgatg accgtctcca cggtgaaatc cagcagggag
     5281 tcggcgccca gcaccgaacc ggcctgggag ccgatcttca ccgccacctg gggacggctt
     5341 cgacgggtcc accagtccgg cactcgcacc agtacgcctg cctcttcgta gaggggcaca
     5401 tccttgagga aggcgtaagc atcatccggc gtccacgcca ggggctggta gatgtcgctg
     5461 gaatccacga gcgcccggat gagatggctc ttctccgcgg ctcgctggac cggcgtcagg
     5521 agatggacca gcgcttttcg gttgcgctcc ccggcgagct cctgcagcgc ccgacccagc
     5581 ggctggtagc ggatccgggc ggactcggta agccgcggcg cgtaggtggc caggaacgcg
     5641 aacgggtact cggcgtcctt cttgttctcc gcgaggtgaa aacacactcg cccaacctgc
     5701 cgccacaggg gcgcgtgagc cgtcaggaac gtttccaggc tgcccgccgc ggaagcctga
     5761 cctcggaccc attcgtccag ggaggaccac aacccgacga gcacgtcagc ggagagatac
     5821 tccacacctt gcgtcggagg cacggacagg acccgagcca gcagctcatc cggtcccggc
     5881 ggaggaatgg gctctggctg ggaggtgccg gcaggagtgt ggcagagggc gcccaggtag
     5941 agggccgcga actcccgcca gtaggcaagc tctggaggca gcccgccgga gatgggacgc
     6001 gcggcaagct cgaagagtcc ttccgcgggg ctgacgcgga aggcttccag tacccgccgg
     6061 aggcccggcg ccatcccggg acggtcggca accgcggagg cgaccatcag atgccctccg
     6121 ggggtgatgg agagcagacc gtcgagagga ggttcgtcgc ccacgagccc gagtctatcg
     6181 atccgtcacg ctcacgtcac cggtattgtc acgtcaccgg tacgcccggc gttgccgatc
     6241 gagacccacg cccaggcgct atcgccggag tccagggagc gcgccgcggc cggcttgccg
     6301 ttcacggagg ctgcgtggac cacggttccc cgagggaagg ggccgaagcg gacgctcagc
     6361 cgatcggtcg ggcgctccag gtcgtagcgc aaggtgtgct tgccgccgtc gcgctcgtag
     6421 acgtaggcca gcctctgacg cgccgagccg gtgagcaccg ggtattcgga gatcgccatg
     6481 cgagtccagt ccgaaggata ccgtggcacc aggcgcaggt gatccggatc gttgtcgtcg
     6541 atccccagga tcaggcgaac cgccttggtg ctgtccgcca cgtggctgtc ctggcccatg
     6601 tatccgttga cgggcagata gtacttcatg gaccggtgcg tgatgatccc ctccggcgag
     6661 gcccacccgt ggagccgagg cagatagcag taccgcagga gcaggtccag aaaccggccc
     6721 gcatccgtca tctcgtccag gaggagcgcg gcctgggtca tcatcccctg gccgtacccg
     6781 tacatgcgca ggcagttgaa ggtggaggcg gaagcggcac cctcggtgcc tcccgcgccg
     6841 cgccccatca ggtagcggta ggagttgcgg ctgatctcca ggcaccggcg ctccagatcg
     6901 tcgccggctc cgtagtcctg cagcggcgtg taggaatccc cctcggtcga caggtgcagg
     6961 tgcaccagct tgtgggcgtg gtcctgccag tcgcagacgt cctccgtgtg ccagatgggg
     7021 ccgaccggac tggggtccac caggtggtcc aggatgcctt gccgcagcct gcggaccagg
     7081 gcttcccact gatccgccag ggcaccctcc cccagggcgc gcgccatgcg ggccgagagc
     7141 ttggcgccat ggaggcagtt gtaggtggaa tagatgtcgt agtcgccgtg ggcgcactcg
     7201 ctctcggtat agagaacgtc cttgcgcacg ccggggcgca gatggtcggt gtcgagctgc
     7261 cactggatcc agtcgaccgc ggcgcgtgtg gcggcaaagc ggcgccggtt ccactcggct
     7321 ggccggccct tccaatgcca catcatgtac cggccccaca tgcagatgcc gtgtccgtcg
     7381 ttctcccggt tgccgacctc ctcgacctcc ccgacccgaa cggtcttgta accctcggat
     7441 ccgcggccca gtccggcgat ccggatccaa tgcggcgggt tggcctccgc aaagag
//
LOCUS       DASRZS010000308         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_168319, whole genome shotgun sequence.
ACCESSION   DASRZS010000308 DASRZS010000000
VERSION     DASRZS010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168319"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VFH83_07620"
     CDS             complement(<1..628)
                     /locus_tag="VFH83_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET6486273.1"
                     /translation="MTEQTMKAERSEPRARWEPKPPTTIEPLSDETFQRFCKLIYEKT
                     NIRMRESKHVLVSNRLRKRIVALDLKGYDEYYDYLSRGEKRAQEMVHFIDAVSTNETY
                     FFREYNHFDALLKLVLPDLSQRKRRLRFWCAGCSTGEEPYTLRIVLEEARAAHRVGEV
                     EILATDISHQVIDRAREGEYGERAVRMVPADLMKRYFIQEREGCYRVSP"
     gene            complement(625..1110)
                     /locus_tag="VFH83_07625"
     CDS             complement(625..1110)
                     /locus_tag="VFH83_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HET6486274.1"
                     /translation="MGNDVQLVKMAEIGVAENSTHLKTTLGSCVGLILHDPKRSISGL
                     AHIVLPRKTRSDGSVGKYADTAVPALLSEMVKRGSSPSELKAYLTGGASMFPFSGDRR
                     LTSIGDMNVEAVKAILEELRIRLTFVDTGGERGRTIVFKNLTGEILVTTLNRTGDGDK
                     R"
     gene            complement(1124..3283)
                     /locus_tag="VFH83_07630"
     CDS             complement(1124..3283)
                     /locus_tag="VFH83_07630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET6486275.1"
                     /translation="MVRVSVERLDALLNLVGELVIQNSGFHSVGEQLRNKYRGEVLVA
                     DLQSKTEALTKVTRDLQDGIMKVRMLPVNNVFSRFHRVVRDLAKDHGKEITLDIFGEE
                     TEIDKKVMDRIAEPLLHLVRNSADHGIESKEERLAAGKRAVGVIRLGAYQDGDHICVE
                     VSDDGRGLDRDAILKKALEKGLVEKRDAGTLPDEKILGMIFLPGFSTARKVTETSGRG
                     VGMDVVMRAVQSMGGTIRIRSTTGSGTKVTISLPLTMAILPGLLVEVSGSTLAIPLSS
                     VKEVLKVGARELNTVGLRPVIRLRNEVLAVVELEDALGLTEEGSAEAKDERTPVVIVE
                     FEKRKLGLGVGRIVGTGEVVIKSLSRHYREIPGLIGASILGNGKIALIVDVEALVRLY
                     HDPNSQHRAHAGNTVFELLGDAGLRGEVSAEAAPAASAPVSTASTGTTAPAALETPVV
                     PAATAPAAPVVHPTLEAAPPVTSEPAASATPEPAAPATLAPAVPEPAAPAEPEGPAPV
                     SPAELMRELSNGGGALLEEIHNAGAIQASISVSTMTGRDVQVSFPESRAVPLADVGEM
                     LGGEEAAVAGLYVGLNGELDGGILMVIPQETLPALDDLLHKRPVGTCGSLEDVDLSAI
                     SELGNILSAAFLNAMSDGTRLSIRHEPPEIGLDMCLSVIDSVLARFAQPGDELLLTTA
                     ELASEGVEKLTCRLVLFLEPASLRKLVGALAAAESLV"
BASE COUNT          572 a         1287 c         1192 g          657 t
ORIGIN      
        1 tcggagacac ccgatagcag ccttcgcgct cctggatgaa gtagcgcttc atcaggtcgg
       61 ccgggaccat gcgcaccgcg cgctccccat actcgccctc acgcgcccga tcgatcacct
      121 ggtggctgat gtccgtggcc aggatctcca cctcccccac gcggtgggcg gcgcgcgcct
      181 cttcgaggac gatccgaagc gtgtagggct cctctccggt ggaacagccc gcgcaccaga
      241 accgcagccg ccgcttcctc tggctcaggt cgggcagcac cagcttcaga agggcgtcga
      301 agtggttgta ctcccgaaag aagtacgtct cgttcgtgct caccgcgtcg atgaagtgca
      361 ccatctcctg ggcgcgcttc tcgccccggc tcaggtagtc gtagtactcg tcgtaccctt
      421 tcaggtcgag cgccacgatc cgcttccgca gccgattgga gaccagcacg tgcttgctct
      481 cccgcatgcg gatgttggtc ttctcgtaga tcagcttgca gaaccgctgg aaggtctcgt
      541 cggatagcgg ctcaatggtc gtcggaggct tcggctccca tcgagcccgt ggctccgacc
      601 gctcggcctt catcgtttgc tccgtcaccg cttgtctcca tccccggtgc ggttcagcgt
      661 cgtcaccagg atctcccccg tcaggttctt gaacacgatg gtccgtccgc gctcgccccc
      721 ggtatccacg aaggtgagcc ggatgcgaag ctcctccagg atggccttca ccgcctccac
      781 gttcatgtcc ccgatgctgg taaggcgacg gtctccactg aaggggaaca tgctggctcc
      841 acccgtcaga taggccttga gctcggatgg gctgcttccc cgtttcacca tctcggagag
      901 gagcgcgggg accgcggtat ccgcgtactt ccccacggaa ccgtccgagc gggtcttccg
      961 tgggagcacg atgtgcgcca gaccgctgat gctccgcttg ggatcatgga ggatcaagcc
     1021 cacgcaggac cccagggtgg tcttcaggtg cgtggagttc tccgccacgc cgatctcagc
     1081 catcttcacc agctgcacgt cgttccccat gacatccgcc cgatcagacg aggctctccg
     1141 ccgcggccag ggctccgacc agcttgcgca gcgacgccgg ctccaggaag agcaccagcc
     1201 tgcaggtgag cttttccacc ccttcggagg ccagctccgc cgtggtcagg agcagctcgt
     1261 cgcccggctg ggcaaaacgc gccaatacgg agtctatcac ggatagacac atgtccagtc
     1321 cgatctcggg cggctcgtgg cggatggaca gcctggtgcc atccgacatg gcgttgagaa
     1381 acgccgcgga gaggatgttc ccgagctcgg agatggcgga cagatccacg tcctccaggc
     1441 tcccgcaggt tccgacgggt ctcttgtgga gcaggtcatc cagagccggc agggtttcct
     1501 gtgggatgac catcaggatc ccgccgtcca gctcaccgtt caacccgacg tacagcccgg
     1561 ctacggcagc ctcttcgcct cccagcatct cccctacgtc cgccagcggg acggctcggg
     1621 attccggaaa gctcacctgg acgtcccggc cggtcatcgt ggaaacggag atggaggcct
     1681 gaatggctcc cgcgttgtgg atctcctcca gcagcgctcc tccgccgttg gaaagctcgc
     1741 gcatcagctc ggcaggggac accggagccg ggccctccgg ctccgcaggg gccgcgggct
     1801 cagggacggc gggcgcgaga gtcgcggggg ccgcgggctc gggagtcgcc gaggcggcag
     1861 gctccgaagt caccggggga gcggcctcga gggtcggatg aaccaccgga gctgccgggg
     1921 ccgtggcggc tgggaccacg ggcgtctcga gggctgccgg ggccgtagta ccggtagacg
     1981 cagtcgaaac cggcgccgat gccgcgggag ccgcttcggc ggatacttcc cctcggagcc
     2041 cggcgtctcc caaaagctcg aacacggtgt tgccggcgtg ggcgcgatgc tggctgttgg
     2101 gatcgtgata cagccggacc aacgcctcca cgtccacgat cagcgcgatc ttgccattcc
     2161 ccagaatcga ggccccaatc agacccggga tctcgcggta atgacggctc agggacttga
     2221 tcaccacttc cccggtgcct acgatccgcc cgaccccgag cccgagcttg cgcttctcga
     2281 actccacgat caccacgggc gtcctctcgt ccttggcctc cgccgagccc tcctcggtca
     2341 acccgagggc gtcttccagc tcgaccaccg ccagcacctc gttgcgcaga cggatcacgg
     2401 gccgtagacc gacggtgttc agctcccggg cgccgacctt gagcacttcc tttacggatg
     2461 agagcgggat cgccagggtg gacccgctca cctccaccag gaggccgggc aggatggcca
     2521 tggtgagggg cagggagatg gtgaccttcg tgccgctgcc cgtggtggag cggatgcgga
     2581 tcgtgccgcc catgctctgg accgctcgca ttaccacgtc catgcccacg cctcggccgg
     2641 aggtctcggt gaccttccga gccgtagaga aaccgggcag gaagatcatg cccaggatct
     2701 tttcatccgg cagcgtcccg gcatcccgtt tctccaccag acccttctcg agggccttct
     2761 tcaggatggc atcccgatcc agcccccggc cgtcgtcgga gacctcgacg cagatgtgat
     2821 ccccgtcctg gtaggcgccc aggcggatga cgccgacggc gcgctttccg gccgccagac
     2881 gctcttcctt ggactcgatc ccgtggtccg ccgagttccg caccaggtgc agcagaggct
     2941 ctgcgatccg gtccatgacc ttcttgtcga tctcggtttc ctcgccgaag atgtccaggg
     3001 tgatttcctt gccgtggtcc ttggcgaggt cccggaccac gcggtggaac cggctgaaca
     3061 cgttgttcac cgggagcatc cggaccttca tgatcccgtc ctgcaggtcc cgcgtgacct
     3121 tggtcagcgc ctcggttttg ctctgcaggt cggcgaccag aacctcgccg cgatacttgt
     3181 tccgaagctg ctcacccacg gagtggaagc ccgagttctg gatcaccagc tctcccacca
     3241 ggttcagcag cgcgtccaga cgctccacgg acacgcgcac cacgtcttcg cctttgaatg
     3301 cgcgcgtttc ggcgggcgcc gctgccgccg gagttgccgc cggagcggcc gctgccctgg
     3361 aggccggctg cgccggcggg gaagcggcgg tcgaaacctc cgcggcaggt ggaagcggtg
     3421 ccgtctctgc cggcgggacg gcagcgaccg gctgtggcgc aaccgcggca ggctgcgctg
     3481 gcgccgtagc aggctgcgct ggcgccgtag cgggctgcgc tggcgccgta gcaggctgcg
     3541 ctggcgccgt agcaggctgc ggcgtgaccg gggcgttcga gacagactgc accgccggcg
     3601 tcgcgctgga cgggggcagc tcctcccgcg agatctgtct catggaagtg atgcgcagct
     3661 ggtcgacgag cagcgtatgc tggaggtccg tctcctcctg gtccgtgt
//
LOCUS       DASRZS010000309         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1709946, whole genome shotgun sequence.
ACCESSION   DASRZS010000309 DASRZS010000000
VERSION     DASRZS010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709946"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VFH83_07635"
     CDS             <1..1248
                     /locus_tag="VFH83_07635"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HET6486276.1"
                     /translation="ARRSGKEPVTYPHPCLEPVLKKTLGIPLFQEQVMKLAVVAADYT
                     PGEADQLRRDMAAWKQKGRIEHHHDRLVSRMVAKGIQPEFAERVFGQILGFGEYGFPE
                     SHAASFALIAYATAYVKHHHPVVFACALLNAWPFGFYSPATVIDDSKRHKVIFLPMDI
                     LRSDWDCTLERIPGADATRDYAVRIGLRYLRGLGEGDWDRIRAWRAGGRTSTFQDFTS
                     LCRLPSDSMERLAEIGAFDCFDRGRRQALWGVLDQSQCPTNLLVEENEEPPSFRALSA
                     AEEITWDYLASDQSPRGHVMEQWRPLLQNQGLGDAAAISRLDHGRKVSLVGCAICRQM
                     PGTAGGVLFMTLEDESGFANVVVWEKVFKQYRSMILTNWLLGVTGKLQVADGVVHIIA
                     DSFWKPDFIRTEAGVAAASRDFR"
     gene            1317..2348
                     /locus_tag="VFH83_07640"
     CDS             1317..2348
                     /locus_tag="VFH83_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET6486277.1"
                     /translation="MPRPTQAGAAEPAVRTTVVGSYPVPSWLGPAPTLPNLRDAVMVV
                     LKTQELAGVDVVSDGELTRFDASHPDTNGMIDYFASRMDGIRSRLTREEAARFAARPE
                     MRFRASPAGVVTGPVGQGTLDLPAAWEWSRALTDKPMKLTVTSPYMLARTLLDDHYRD
                     LRALTNALADALAAQVAAVSAPVVQVDEANLPGHPEDAGWASAPINTVLRAARAERGV
                     HLCFGNYGGQSVQTGLWKDLLPFFNTLEADHLVLETAHRDPAELTALRELKPGLSLGL
                     GVIDIKSTVVETADDIARAVERASALLGADRIRWIHPDCGFWMLPRSVADRKLRSLVS
                     GRDRYLGRQ"
BASE COUNT          419 a          846 c          869 g          420 t
ORIGIN      
        1 gccaggcgca gcgggaagga gccggtaacc tatccccacc cgtgcctgga gcccgtgctc
       61 aagaagaccc tcgggattcc cctcttccag gagcaggtga tgaagctcgc ggtggtcgcc
      121 gcggactata cccccggaga ggcggaccag ctccgtcggg acatggcagc ctggaagcag
      181 aaggggagga tcgagcacca ccacgaccgc ctggtttccc gcatggtggc caagggcatc
      241 cagccggagt tcgccgagag ggtgttcggc cagatcctcg ggttcgggga gtacgggttt
      301 ccggaatcgc acgccgcaag cttcgcgctc attgcgtatg ccacggccta cgtgaagcac
      361 caccacccgg tggtgtttgc ctgcgcgctt ctcaacgcct ggccgttcgg gttctattcc
      421 ccggccaccg tgatcgacga ttccaagcgc cataaggtga tattcctgcc catggatata
      481 ctgagaagcg attgggactg cacgctggag cgcatcccgg gcgccgacgc cacccgagac
      541 tatgcggtcc gcatcgggct gcgatatctc cgcgggctgg gagaagggga ctgggaccgg
      601 atccgcgcgt ggcgggccgg cggtcggacc tccaccttcc aggacttcac ctctctctgt
      661 cgcttaccga gcgactccat ggagcgcctt gccgagatcg gggccttcga ctgcttcgac
      721 cggggccgcc gtcaggcgct ctggggagtg ctggaccaga gccagtgccc cacgaacctg
      781 ctggtcgaag agaacgagga gcccccgtcc ttccgggccc tgagcgccgc ggaggagatc
      841 acctgggact acctggcatc ggaccagagc ccccgtggtc acgtcatgga gcagtggaga
      901 ccgctcctcc agaatcaggg gctgggggat gcggccgcga tctcacgatt ggaccacggg
      961 cgcaaggtgt ccctggtggg gtgcgccatc tgccggcaga tgccgggcac cgcgggcggc
     1021 gtcctcttca tgacgctgga ggacgagtcc gggttcgcca acgtggtggt ctgggagaag
     1081 gtcttcaagc agtatcgctc gatgatcctg accaactggc tcctgggcgt caccggcaag
     1141 ctccaggtcg cggatggggt tgtccacatc atcgcggact ccttctggaa gccggacttc
     1201 atccgcacgg aggccggcgt agcggccgcc agccgcgact tccgatagcc cgagccctcc
     1261 gcgttcccgg ggctgccgtt gttgaccgtg cacgcgcgca tggctaccgt ttgtccatgc
     1321 cccgtcccac gcaagccggc gccgccgagc ccgcggtccg aacaaccgtt gtagggagct
     1381 acccggttcc ttcctggctc ggcccggcgc ccacgctccc caatctgcgc gatgccgtca
     1441 tggtggtcct gaagacgcag gagctcgcgg gcgtcgacgt cgtgtccgac ggggagctga
     1501 cccgtttcga tgccagccac ccggacacca acgggatgat cgactatttc gcgtcgcgga
     1561 tggacggcat tcggagccgg ctcacgcggg aggaggccgc acggttcgcg gcccgtcccg
     1621 aaatgagatt tcgcgccagt ccggcgggag tcgtgaccgg ccccgtgggc caggggacgc
     1681 tggacctgcc cgcggcgtgg gaatggtccc gggctctcac cgacaagccg atgaagctca
     1741 ccgtcacctc tccctacatg ctggccagga ctctcctgga cgaccactat cgcgatctgc
     1801 gggcgctgac gaatgcgctt gcggacgcgc ttgccgcaca ggtggccgcg gtgagcgcgc
     1861 ccgtggtgca ggtggatgaa gccaacctgc ccgggcatcc cgaggacgcc ggttgggcct
     1921 cggcgcccat caacacggtg ctccgcgccg ccagggcgga gaggggcgtc catctgtgct
     1981 tcggcaacta cggtgggcag tcggtccaga ccgggctctg gaaggacctc ctgccgttct
     2041 tcaacacgct ggaggcggat cacctggtgc tggaaaccgc ccatcgggat cccgccgagc
     2101 tgacggcgct ccgtgagctc aagccgggcc tctcgctggg tctcggggtg atcgatatca
     2161 agagcaccgt ggtcgagacc gcggacgata tcgcgcgggc cgtggagcgg gcgtccgccc
     2221 tgctgggcgc cgatcggatc cgctggatcc atccggattg cggattctgg atgttgcctc
     2281 gaagcgtggc ggatcgcaaa ctgcggtccc tggtatccgg ccgggaccgc tacctcggtc
     2341 gacaatgaag ccggccgaca atgaggacgg ctggccagga gcgtgaccga caatgaggac
     2401 aatggagatg gacaaccgaa acgccgcgct cgtcgacgcc gatagccccg cgcgcaaggc
     2461 gcgcctcgcc atgagggcag gggaatggac cgggctcacc acctacaagg tcccgggttt
     2521 cgtgcagtgc aatctcgttg tggtcccccg atcg
//
LOCUS       DASRZS010000310         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3633459, whole genome shotgun sequence.
ACCESSION   DASRZS010000310 DASRZS010000000
VERSION     DASRZS010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3633459"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFH83_07645"
     CDS             complement(<1..227)
                     /locus_tag="VFH83_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET6486278.1"
                     /translation="MPRPQLHPSSPAAPYVVIGVGMAAVSSASILISLARIEGVPALA
                     IAALRMLLAAAVAAPLALLRARRELRATPWR"
     gene            368..1318
                     /locus_tag="VFH83_07650"
     CDS             368..1318
                     /locus_tag="VFH83_07650"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET6486279.1"
                     /translation="MEPRASKVAIVGAGAVGATLAYNLALKGTVTELALIDVNLEKAQ
                     AEILDIQQGASLGRSIQMTAGGYEECRDAAITIVTAGARQKPGETRVDLLSRNVGIVR
                     DIVRSSVGAGFQGAFLLITNPVDVLTWVAFAESGFPAARVIGTGTTLDSARLREHVAR
                     HCRVNPTNIHGYVLGEHGDTSFPAWSLLTVGGVHLDEFCPFCQSCVEKDEFRARAAEE
                     IRQAAYRIIEAKGSTSYAIGQAASVIVESIVRDERRILPVSTVRKDYRGIERTAFSFP
                     TIVGRGGAETVLQFTLPPEEDELLLKSARFVAENIVAAGY"
     gene            1405..2595
                     /locus_tag="VFH83_07655"
     CDS             1405..2595
                     /locus_tag="VFH83_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HET6486280.1"
                     /translation="MKPSRIRQLRDSFGSALLQDTMPFWIQHAVDRECGGFLPQLDRR
                     GAVFCPDKPMWIEGRFTWTLSRLYNTVERRSEWLTLAAHGIEFINRHGFDKDGRVFFL
                     VSREGKPLRKRRYLFAEVFAVLAFAEYSRAAGDPKALDTARRLMALIRDTLARPWPQP
                     GSLEPKVNPETRSMRGHSMSMILINTLQVLREADPAGAYDPQIDAQIEEVFRYFVKPE
                     KRALLETVGANGEYLGDLPEGRCVNPGHALETAWFLLIEARRRGDQELVKRALPIIDW
                     SLELGWDRKHGGILYFVDVEGKPAVQYEWDMKLWWVHNEALNATLLAHVITGDRRYEE
                     WFERILRWAHGHFPDKTCGEWFGYLHRDGSVSHDLKGNTWKGPFHLPRQQLFCHLLLK
                     EIAG"
BASE COUNT          468 a          861 c          919 g          409 t
ORIGIN      
        1 tccctccagg gggtggctcg aagctcccgt cgcgcccgaa ggagggccag cggcgcggca
       61 accgcggccg ccagcagcat gcgcagcgcg gcaatggcca gggccggcac gccctcgatc
      121 cgcgcgaggc tgatgaggat cgaggcggaa gatacggccg ccattcccac gccgatcacg
      181 acgtacgggg cggcagggct cgacggatgg agctgcggtc tgggcacggg gggagtgtta
      241 caccatgggc agccgcgtgc ctaccgtgcc gaggccccgt gagggcttcg tcagtatatt
      301 tggtcaccag gaggaagccc gcgaccgccg aacgccaggg tccggcatcc cgagaaagga
      361 agtcatcatg gaacctcgtg cgagcaaagt ggcgattgtc ggggcggggg cggttggagc
      421 taccctcgcc tacaaccttg cgctcaaagg caccgtgacc gagctggccc tgatcgacgt
      481 gaacctcgag aaggcgcaag ccgagatcct ggacatccag caaggggcgt cgctgggcag
      541 gtccatccag atgacggcgg ggggctacga ggagtgccgg gacgcggcca tcacgatcgt
      601 aaccgcgggg gcccggcaga agcccgggga gactcgggtc gatcttctga gccggaacgt
      661 cgggatcgtg cgggatatcg tgcgaagctc cgtaggtgcc ggcttccagg gcgccttcct
      721 gctgatcacc aaccccgtgg acgttctcac gtgggtggcc ttcgccgaga gcggcttccc
      781 cgcggcccgc gtgatcggaa ccggtactac gctggattcc gcccggctgc gggagcacgt
      841 tgcccggcac tgccgggtca accccacgaa catccacggg tacgtgctgg gcgagcacgg
      901 ggacacctcg tttccggcct ggtccctgct gaccgtcgga ggggtgcacc tggacgagtt
      961 ctgccccttc tgtcaaagct gcgtagagaa ggatgagttt cgagcccgtg ccgccgagga
     1021 gatccgacag gccgcctacc ggatcatcga ggccaagggc tccacgtcgt acgccattgg
     1081 ccaggcagcc tcggtcatcg tggagtccat agtaagagat gaacggcgca tccttcccgt
     1141 gtccacggtg cggaaagact accgcgggat cgaacggacc gccttcagct tcccaacgat
     1201 cgtgggacgg ggcggcgccg agacggtgtt gcagttcacg cttcctccgg aggaggacga
     1261 gctgctgctg aagagcgccc gtttcgtcgc ggagaatatc gtggccgcgg gatactgacg
     1321 ctgccaggcc gcgcgctacg ggcggccgcg ccggcagcac gccgtttcgt tgacaggggg
     1381 cacggccggg cataggatcg gcccatgaaa ccatcccgca tccggcagct acgcgactcc
     1441 ttcggctcgg cgcttctcca ggacaccatg ccgttctgga tccagcacgc ggtggatcga
     1501 gagtgcggcg ggttcctccc ccagctcgac cgacgcggag cggtgttctg cccggacaag
     1561 cccatgtgga tcgagggacg gttcacctgg acgctctccc ggctgtacaa caccgtcgaa
     1621 cgtcgatcgg agtggctcac gctggcggct cacgggatcg agttcatcaa ccgtcacggg
     1681 ttcgacaagg acggtcgggt tttcttcctg gtcagccgcg agggaaagcc gcttcgcaag
     1741 cgtcggtacc tgttcgcgga ggtcttcgcc gtcctggcgt tcgccgagta ctccagggcc
     1801 gccggtgatc cgaaggcgct ggacaccgct cgacgcctga tggccctgat ccgggacacg
     1861 ctggcccgcc cctggccgca gcccggcagc ctggagccca aggtgaaccc cgagacccga
     1921 tccatgcgcg ggcactcgat gagcatgatc ctcatcaaca cgctgcaggt gctgagggaa
     1981 gcggatcccg ccggcgccta cgacccgcag atcgacgctc agatcgaaga ggtgttccgc
     2041 tatttcgtga aaccggaaaa acgggcgctc ctggagacgg tcggcgccaa cggcgagtac
     2101 ctgggggacc tgccggaggg acgatgcgtc aacccggggc atgcgctgga gaccgcgtgg
     2161 ttcctcctca tcgaggcgcg ccggcggggg gaccaggagc tggtcaaacg cgcgctcccc
     2221 atcatcgact ggtccctgga gctggggtgg gaccgcaagc acggcggcat cctctatttc
     2281 gtcgacgtcg agggcaagcc cgcggtccag tacgagtggg acatgaagct gtggtgggtg
     2341 cacaacgagg cgctgaacgc cacgctcctg gcccacgtca tcaccggtga ccgccgctac
     2401 gaggagtggt tcgagcggat cctccgctgg gcccacggac acttcccgga caagacctgc
     2461 ggggagtggt tcggctatct ccatcgggac ggcagcgtat cccacgacct gaagggcaac
     2521 acctggaagg gccccttcca cctccctcgc cagcagctct tctgccacct gcttctgaag
     2581 gagatcgccg ggtagcccgg caggagggac catggcagca cggaagcgat acgcccaggt
     2641 aggcaccggg ggacggg
//
LOCUS       DASRZS010000311         5908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1328149, whole genome shotgun sequence.
ACCESSION   DASRZS010000311 DASRZS010000000
VERSION     DASRZS010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328149"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFH83_07660"
     CDS             complement(<1..302)
                     /locus_tag="VFH83_07660"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HET6486281.1"
                     /translation="MAKAWFVVHTYSGYENKVQKHVRLLVDKGTLKEHVFDVKVPSEE
                     VVEIKDGHKKVSTKKFLPGYILVEMDLPDQGWKEICQEIRKIPGVTGFVGSNRNQKP"
     gene            complement(314..493)
                     /gene="secE"
                     /locus_tag="VFH83_07665"
     CDS             complement(314..493)
                     /gene="secE"
                     /locus_tag="VFH83_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET6486282.1"
                     /translation="MKRIILFFQESFAELRKVTWPSRDEAVASTKVVLVSSLIIALFL
                     GLVDFILFKIVDALF"
     gene            complement(526..598)
                     /locus_tag="VFH83_07670"
     tRNA            complement(526..598)
                     /locus_tag="VFH83_07670"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(563..565),aa:Trp,seq:cca)
     gene            complement(614..790)
                     /gene="rpmG"
                     /locus_tag="VFH83_07675"
     CDS             complement(614..790)
                     /gene="rpmG"
                     /locus_tag="VFH83_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET6486283.1"
                     /translation="MAKGKAKNLELVALQCTECDRRNYTTTKNRKNTQGKLEFSKYCR
                     WDHKHTLHKEAKVK"
     gene            complement(942..1014)
                     /locus_tag="VFH83_07680"
     tRNA            complement(942..1014)
                     /locus_tag="VFH83_07680"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(979..981),aa:Thr,seq:ggt)
     gene            1193..3028
                     /locus_tag="VFH83_07685"
     CDS             1193..3028
                     /locus_tag="VFH83_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486284.1"
                     /translation="MSIKKVLVVSLMLPVLLLAMGCPLPISPPHSTQETTSPPAATGQ
                     SGSLSVNVTAAAPWLWPLLGQGGSKAFLFATSVKVSLKTSGGSDVISPVTLSLSLDAS
                     GSGAGTSTLDTINNIPAGSNYTLKVDLYNSVVSTTTAMVEGQATVSITTGNTTSTTVV
                     CTPLGATSISVGSLTPVTQSLAQYGEKWYSIVMGAGTYTINQTGASTASFLFTPAGEL
                     IGQVSVGTPYTYPCATAGTYFLGVAGLAAATSSVTVSGPPSNEGSIASPVAVTLDAAR
                     TFKIGVSAAAQDYSYYTFTTTSAGTYYFDVSQLYSYYLYVYTNSDFTGSVVSSVNGAW
                     GVGFSLTGSTTYYLKLRNTSSSVASFSGMVVDPAYAASHTQGEGSVASPIGLTPEVGH
                     AATLGWHAYDYKSYYTFTTGSDTDFTFSISGYAPSGTYLYAYVGTDSTYASYSSSGYL
                     SSSSTFTASLSPSTQYYLLLVNETYPYTTAATATLLAHAAPTVITPLSLSTLTVGQLL
                     TSSSVVWYEVSGLTPGASYAVFWDDSFAGSGTYTGDLLVDAYHQDRVTTYWTGMDSAY
                     TTPVSVIVAGTDTKFYLRVRGFGGDTGTFGIRVDPNGGSLAITVH"
     gene            complement(3120..3656)
                     /gene="rsmD"
                     /locus_tag="VFH83_07690"
     CDS             complement(3120..3656)
                     /gene="rsmD"
                     /locus_tag="VFH83_07690"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET6486285.1"
                     /translation="MRVTGGLYRGRTILCPPGDIRPSMDRMRISLFSILGDLSGTSFL
                     DLFAGTGVIGVEAASRGAEPVLLVEKDARKRSTIERNISFVQSGIELVLAPVERFLRN
                     NTRSWDTIFLDPPFAFAEKKAILEAACAVPHLAPEGLSILHLHRAEAQQIEPAGLTLV
                     DRRAYGQSVLLFYRRAEG"
     gene            complement(3678..5744)
                     /gene="recG"
                     /locus_tag="VFH83_07695"
     CDS             complement(3678..5744)
                     /gene="recG"
                     /locus_tag="VFH83_07695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET6486286.1"
                     /translation="MFLGELTQEVSSLKGVGPALAGRLERLGVRTIEHLLLTLPRGYE
                     DRTAVVPLAQAAGLERACVVVTVTSLTDVGWGRSRVPKAAIQDASGQAVLVAFGRPFL
                     RSTLQPGRTFFVYGAFRARRGELSCSDFELEPWSEQPMSFGKVLPLYPLTEGMTQSAM
                     RKLLRVALDQHAAHLESRLPAALVRRKALMPTAEALRGVHFPTSEDHARRCREVLAYE
                     ELFYFELMVLRRKSALAVTRSARKSLDPALRDRLIARLPFHLTEDQKTVCAEIAGDLA
                     SAAPMARLLQGEVGCGKTLVAFLSALLVIGGREQVAFIAPTELLARQHAENAARLLEP
                     LGIRMAFLSGAVTGEPRELLLAALRAGDIDLLFGTHALFSQDVAFRSLGLVVVDEQHR
                     FGVLQRQALLAKGANPDMLLMTATPIPRTLALTAFGDLDVSTIRTMPAGRKAVITHLT
                     REGNEEKVYERVRQEVERGRQAYFVYPMIGQSESLAVKDAETAYRLLKEKVYPGVSTA
                     LIHSKLAEDQKAAGMAGFATGAIRILVATSVVEVGVDVANATCMVVEHAERFGLSTLH
                     QLRGRVGRGSEQSYAFLVYGNQLTPEGVQRLRIMKETTDGFVIAERDLALRGPGELLG
                     VRQSGFLNFRAADLLVHAHQLLEARDDVRALLAEDPGFLAPENSVVQRVLTAAPPFPV
                     AAMEGG"
BASE COUNT         1054 a         2008 c         1821 g         1025 t
ORIGIN      
        1 ggcttctggt ttcggttgga accgacgaaa ccggtcaccc cagggatctt gcgaatctcc
       61 tggcagatct ccttccatcc ctggtccggc agatccatct ccaccaggat gtagccggga
      121 aggaacttct tggtcgagac cttcttgtgc ccgtccttga tctctaccac ttcctccgaa
      181 ggcaccttga cgtcgaacac gtgctccttg agcgtgcctt tgtccaccaa cagacggacg
      241 tgcttttgta ccttgttctc atacccggag taggtatgga ccacgaacca cgcttttgcc
      301 atgcggccgc cgtctaaaaa agcgcgtcca cgatcttgaa gaggatgaag tccacgagac
      361 ccaggaacag tgcgataatg agagaagata ccaaaacgac cttggtggag gccactgcct
      421 cgtcccggct gggccaggtg actttcctca gctccgcgaa gctctcttgg aagaacagga
      481 tgattctctt catatcctcc gtcctgggat cccctgatca cgctacaggc caggcaggac
      541 tcgaacccgc aacatccggt tttggagacc ggcgctctac cattagagct actggcctat
      601 gctacgaaaa cccctacttt accttcgctt ccttatggag ggtatgcttg tgatcccagc
      661 ggcagtactt ggagaactcg agcttcccct gcgtgttctt gcggttcttg gtggtcgtgt
      721 agttgcgccg atcacactcc gtacattgca gcgcaacgag ctcgaggttc ttcgcttttc
      781 ccttggccat gctggtctcc ctgggggttt gttgaaggac aaatgtatcc actttcgccc
      841 ccatgtgtca agtggacaca ctttcttcac tccctgcgcg gcgcgccgcg cgatgctgga
      901 ttgagacgcc gatccgacga aagcccccac aagaggcttt gagccctcga ccggatttga
      961 accggtgacc ccgtccttac caaggacgtg ctctaccagc tgagctacaa gggctttccc
     1021 aagcagcgga atcctaccag aagccgctcc ggttggtcaa ggccggcccc cggggaaagg
     1081 ctcagggtta cccggcgcga caggaggggt ccgaactgaa atgccgcttc cgacgggata
     1141 cggttatctc tgatttgtca attcttaacg ttatataaca aaggagtaag acatgagcat
     1201 caaaaaggtc ttggtagtct ccctcatgct tcccgtactg ctccttgcga tgggctgtcc
     1261 gctgccgatc tccccgcccc actccaccca ggagaccacg tcccctccgg cagcgacggg
     1321 acagtccgga agcctgtccg tcaacgtgac cgccgccgct ccctggctct ggccgctgct
     1381 ggggcagggt gggtccaagg cattcctctt cgcgacctcg gtcaaggttt ccctgaagac
     1441 ctccggtggc tccgacgtga tctcgccggt cactctctcc ctgtccctgg acgcctccgg
     1501 gtcgggggcg gggaccagca cgctggatac catcaacaac atccccgccg gctcgaacta
     1561 cacgctgaag gtcgacctgt acaatagcgt ggtctctacc acgacggcga tggtcgaggg
     1621 acaggctacg gtgtcgatca cgacaggcaa cacgacttcc acgacggtcg tctgcacccc
     1681 tttgggagcg accagcatct ccgtcggctc tctgactccc gtgacccaga gtctggctca
     1741 atacggcgag aagtggtact cgatcgtgat gggcgccgga acgtacacga tcaaccaaac
     1801 cggcgccagc acggcgagct tcctgttcac ccccgccgga gagttgatcg gccaggtcag
     1861 cgtcgggacc ccctacacct acccgtgcgc caccgccggc acctacttcc tgggcgttgc
     1921 cggtctggca gccgccacct cttccgtgac cgtgagcggc cctccgagca acgagggttc
     1981 cattgcctca ccggttgccg tgacccttga cgcggcgcgc accttcaaga tcggcgtctc
     2041 cgccgccgct caggactaca gctactacac gttcacgacg acgagcgcgg ggacctacta
     2101 cttcgacgtt tcccagctct acagctacta cctctacgtc tataccaact cggatttcac
     2161 gggcagtgtc gtttcctccg tgaacggcgc ctggggggtg gggttcagcc tcacgggttc
     2221 gacgacctac tacctgaagc ttcgcaacac gagcagctcg gtggcatcct tctccggcat
     2281 ggttgtggac cccgcgtacg ccgcgtccca cacccagggt gaaggctccg ttgcgtcccc
     2341 catcgggctg accccggagg tgggccatgc ggccaccctc ggctggcacg cctacgacta
     2401 caagagctac tacaccttca cgaccggctc ggacacggac ttcacgttct ctatctcggg
     2461 atacgcgccg agcgggactt acctctatgc ctacgtggga acggactcca cgtatgccag
     2521 ctactcgagc tcgggttacc tgtccagcag ctccaccttc acggcgtctc tctccccctc
     2581 gacccagtac tacctgctcc tggtcaacga gacctacccg tacaccactg ccgccaccgc
     2641 cacgctgctg gcgcacgcgg cgcccacggt gataacgccc ctgtcgctca gcacgctcac
     2701 cgtagggcag ctactcacct cctcgtccgt cgtgtggtac gaggtctcgg ggctcacgcc
     2761 gggcgcgtcg tacgcggtct tctgggatga ttccttcgcg gggagcggga cctacaccgg
     2821 cgacctgctg gtggacgctt atcaccagga ccgagtgact acctactgga cgggaatgga
     2881 cagcgcctat accacgcccg tcagcgtgat cgtcgcgggt acggacacca agttctacct
     2941 gagggtaagg gggtttggcg gagacaccgg caccttcggc atccgagtgg accctaatgg
     3001 aggcagcctg gccatcacgg ttcactagaa ccgcccaggc tgcctggaaa tcgccaaacc
     3061 agaagggccg acggtgcgca agcgccgtcg gccctattcg ttactggcgg gggcagcggt
     3121 cagccctcgg cccgccggta gaagaggagc accgactggc cgtaggcgcg ccgatccacc
     3181 agcgtgagac ccgcgggctc gatctgctgc gcttcggcgc gatggagatg caggatggac
     3241 aacccctccg gggcgaggtg ggggacagcg cacgccgctt cgaggatcgc ctttttctcg
     3301 gcgaacgcga acggtggatc caggaagatc gtatcccagg accgggtgtt gttgcggagg
     3361 aagcgctcga cgggcgcaag gaccagctcg atcccggatt gcacgaagga gatgttccgc
     3421 tcgatagtgg accgcttgcg cgcgtctttc tccaccagga ggaccggctc cgcgccgcga
     3481 gaggctgcct cgacgccgat gacccccgta cccgcgaaca ggtcgaggaa ggaggtcccg
     3541 ctcaggtcgc cgaggatcga gaaaagcgag atgcgcatcc gatccatcga gggacgaatg
     3601 tcccccgggg gacagaggat ggtccgtccc cgatagagcc caccggtgac tctcaccccc
     3661 cctcgattct gcccacccta ccccccctcc atcgccgcca ccgggaaggg gggcgccgcg
     3721 gtcaggacgc gctggaccac cgagttctcc ggcgccagga acccggggtc ttccgccagc
     3781 agcgcccgta cgtcgtcccg cgcctccagg agctggtggg cgtggaccag aaggtccgcg
     3841 gcgcggaagt tgagaaagcc gctctggcgc acgcccagaa gctcgccagg gcctcgaagc
     3901 gccagatccc gttcggcgat cacgaaacca tccgtggtct ccttcatgat ccgaaggcgc
     3961 tggacgccct cgggcgtgag ctgattgccg tacaccagga aagcgtagga ctgctcgctc
     4021 ccgcgcccca ccctgccgcg aagctggtgc agcgtcgaga gaccgaaccg ctccgcgtgc
     4081 tccaccacca tgcaggttgc gttcgccacg tcgaccccga cctccaccac gctggtggcg
     4141 accaggatcc ggatggcacc ggtggcgaac cccgccatgc cggccgcctt ctgatcctcc
     4201 gccagcttcg agtggatgag cgcggtggac acgccgggat agaccttctc cttgagcaac
     4261 cggtaggcgg tctcggcgtc cttgaccgcc agggactcgg actggccgat catcgggtac
     4321 acgaagtatg cctggcgtcc gcgctcgacc tcctgccgca cgcgctcgta gaccttctct
     4381 tcgttgccct cccgggtgag gtgcgtgatc acggccttgc gcccggcggg catggttcgg
     4441 atcgtggaga catccaggtc cccgaaggcg gtaagggcca gcgtccgcgg gatcggggtg
     4501 gccgtcatga gaagcatgtc cgggttggcg ccctttgcca ggagcgcctg gcgctggagg
     4561 acgccaaagc gatgctgctc atccacgacc accagcccga gcgagcggaa ggccacatcc
     4621 tgcgaaaaaa gggcgtgcgt gccgaacagg aggtcgatgt ctcccgctcg cagggcggcc
     4681 aggagcagct cccgcggctc cccggtgacc gcgccggaaa ggaaggccat ccggatgccc
     4741 aggggctcca gcagccgcgc ggcgttctcc gcgtgctgac gcgccaggag ctccgttggc
     4801 gcaatgaacg ccacctgctc ccggcctccg atcaccagga gcgccgagag gaacgccacc
     4861 agcgtcttcc cgcatcccac ctcgccctgc aggaggcgag ccatgggtgc cgcggaggca
     4921 agatctccgg caatctccgc gcagaccgtc ttctggtcct ccgtgaggtg aaacggcagg
     4981 cgcgcgatca gccggtcccg cagcgccggg tccagagact tgcgcgcgga ccgggtcacc
     5041 gccagggcgc tttttcgccg cagcaccatg agctcgaagt agaagagctc ctcgtaggcc
     5101 aggacctcac ggcagcgtcg ggcatggtct tcgctcgtcg ggaagtgcac gccccggagc
     5161 gcttccgccg tgggcatgag cgccttgcgc cgcaccagcg ccgccggaag ccgggactcc
     5221 aggtgggccg cgtgctggtc cagggccacg cggagcagct tgcgcatcgc ggactgggtc
     5281 atcccctccg tgagcgggta aagcggcagg accttgccga aggacatcgg ctgctcgctc
     5341 catggctcca gctcgaagtc ggagcacgag agctcgccgc gtcgggcgcg aaaggcgcca
     5401 tagacgaaga acgtccgacc aggctggagc gtgctgcgga ggaacggccg gccgaaggcg
     5461 accaggaccg cctgaccgct cgcgtcctgg attgccgcct tgggcacgcg ggaacggccc
     5521 cagcccacgt cggtgaggga cgtgacggtg accacgacgc acgccctctc cagtccggcg
     5581 gcctgggcca gcggcacgac cgcggtccgg tcctcgtagc ctcggggcaa ggtgaggagc
     5641 aggtgctcga tcgtccggac gccgagacgc tccagcctgc cggccagcgc gggcccgacg
     5701 cccttcagac tggagacttc ctgggtgagc tcgccgagaa acaccgggcc ctccgccgac
     5761 gctgtcgcgg gacgtgcgcc gtggcggcgg ctagatcggg atcatggtga ggagagagac
     5821 gatctggcgg accgccagct ctccgagaag ccaggctacg aacaggagcc cgatggtaag
     5881 gagcaggtgc tgcgtgtagc ccgagcgg
//
LOCUS       DASRZS010000312         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_560499, whole genome shotgun sequence.
ACCESSION   DASRZS010000312 DASRZS010000000
VERSION     DASRZS010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560499"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /locus_tag="VFH83_07700"
     CDS             <1..1480
                     /locus_tag="VFH83_07700"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6486287.1"
                     /translation="KRFTGVHALRQVDFDLEEGEIHALVGENGAGKSTLMKVLIGIAP
                     KDYGEIHYLGHLFNPRDPKHALEMGIGIIHQELNMMEHLTVAENIFIGRESTRGVFLD
                     RRAQDERAAELFRRLKMSIDPTEPLSRLTVGKQQMVEIAKAVSHQLRILILDEPTAAL
                     TDAEIDELFSIMRDLASRGVGMIHISHRLDEIHRIADRVTVLRDGERMGTRKTSEVSR
                     PEIINMMVGRVISFQPKTRSDVPPGARVVLKVNHLNAGRLVRDVSFELRQGEILGVAG
                     LMGSGRTETARAIFGADEVQSGSIEVRGRRALIRSPEDAVAHGIGYLSEDRKRYGLAV
                     NLSVRDNLAMATYDRFQKGLFVHAAAVRDVTQDYIRKLNIKTPSLEQLLRNLSGGNQQ
                     KVVIAKWLIRACDVLIFDEPTRGIDVGAKSEIYALMTELTREGKSIIMISSELPEILR
                     MSDRILVMSEGRITGELPIEKASQEAIMELATIGHQAVLSRS"
     gene            1484..>2141
                     /locus_tag="VFH83_07705"
     CDS             1484..>2141
                     /locus_tag="VFH83_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486288.1"
                     /translation="MPDTIKETSLLQRFRERGLKWALAPGALVILYLFFGAFGINYFS
                     YPTLVNIIDAAYYFGFIAMGVTFVIISGGIDLSIGTVMMCAAITGGTAIKNGLPMVPS
                     LLLIIAVGLAFGAFNGVLVSRLKMPPFIVTLGTMMISMGVGSIVSNVSSATFPIRGQP
                     GGWFKSIFLFIGDNGATIPTGALLVAIVAVICHLMLSNTKLGRYTYAIGSNLEAARLS
                     G"
BASE COUNT          392 a          686 c          685 g          378 t
ORIGIN      
        1 caagcgcttc acgggcgtgc acgccctgag gcaggtggac ttcgacctgg aggaaggcga
       61 gatccacgcg ctggtggggg aaaacggcgc cggcaagtcc acgctcatga aggttctcat
      121 cggcattgcg ccgaaagact acggtgagat ccattacctg ggccacctgt tcaatccgag
      181 ggacccgaag cacgccctgg agatgggcat cggaatcatc caccaggaac tgaacatgat
      241 ggaacacctg acggtggcgg agaacatctt catcggcagg gagtccaccc gcggcgtgtt
      301 cctggaccgg cgcgcgcagg acgaacgggc cgcggagctg ttccgccggc tgaagatgtc
      361 catcgatccc accgagcccc tctcccgcct cacggtgggc aaacagcaga tggtggagat
      421 cgccaaggcc gtgtcgcatc aactgcgcat cctcatcctg gacgagccga ccgccgctct
      481 gacggatgcc gagatcgacg agttgttttc catcatgagg gatctggctt cccggggcgt
      541 gggcatgatc catatctcgc accggctgga cgagattcac cgcatcgcgg accgcgtcac
      601 cgtgctgagg gacggcgaac ggatgggaac ccggaagacc tcagaggtga gccggccgga
      661 gatcatcaac atgatggtcg gccgcgttat ctcgttccag ccgaagacgc gcagcgacgt
      721 cccgcccggc gcccgggtcg tcttgaaggt aaaccacttg aacgccggtc ggctcgtgcg
      781 ggacgtcagc ttcgagcttc gccaggggga gatcctcggc gtcgccggcc tgatgggctc
      841 cggtcggacc gagaccgcgc gggccatctt cggcgccgac gaggtgcaga gcggcagcat
      901 cgaggtccgc ggacggcgcg cgctcatccg gtctcccgag gacgcggtgg cgcacggaat
      961 cgggtacctt tccgaggacc gcaagcgcta cgggctggcg gtcaacctga gcgtgcggga
     1021 caacctggcc atggctacct acgaccggtt ccagaagggg ctgttcgttc acgccgccgc
     1081 ggtccgcgat gtcacccagg actacatccg gaaactgaat atcaagacgc cgtccctgga
     1141 gcagctcctg cgcaatctct ccggcggcaa ccagcagaag gtggtcatcg cgaaatggct
     1201 gatccgcgcg tgtgatgtcc tcatcttcga cgagccgacc cgtggcatcg atgtcggggc
     1261 caagagcgag atctacgccc tgatgaccga gctcacgcgg gaaggaaagt cgatcatcat
     1321 gatctcatcg gagctgccgg agattctgcg catgagcgac cgcatcctcg tcatgtccga
     1381 gggccggatc accggggagc ttcccatcga gaaggcgagc caggaggcca tcatggagct
     1441 cgccacgatc ggtcaccagg ccgttctctc caggagttga cgcatgccgg acacgatcaa
     1501 ggaaaccagt cttctgcagc ggttccgcga acgcggcctg aaatgggcgc tcgccccggg
     1561 ggcgctcgtg atcctgtacc tgtttttcgg ggcgttcggg atcaactact tctcctatcc
     1621 cacgctggtg aacatcatcg acgcggccta ctacttcggg ttcatcgcca tgggcgtgac
     1681 gttcgtcatc atcagcggcg gcatcgacct gtccatcggg acggtcatga tgtgcgcggc
     1741 gatcaccggc ggcactgcga tcaagaacgg gcttccgatg gttccctcgc tcctgctcat
     1801 catcgcggtc gggctggcgt tcggcgcgtt caacggcgtc cttgtctcgc ggctgaagat
     1861 gcccccgttc atcgtgacgc tcggcaccat gatgatctcc atgggcgttg gctccatcgt
     1921 gtccaacgtc tccagcgcca cgtttcccat ccgcgggcag cccggcggct ggttcaagag
     1981 catcttcctg ttcatcgggg acaacggggc cacgattccc acgggggcgc tcctggtggc
     2041 catcgtggcc gtgatctgcc acctgatgct gtccaacacg aagctgggcc ggtacaccta
     2101 cgccatcggc agcaacctcg aagcggcccg attgtcgggc g
//
LOCUS       DASRZS010000313         3885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_561116, whole genome shotgun sequence.
ACCESSION   DASRZS010000313 DASRZS010000000
VERSION     DASRZS010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561116"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VFH83_07710"
     CDS             complement(<1..733)
                     /locus_tag="VFH83_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HET6486289.1"
                     /translation="MSQDLYLIDGYSVIYRGYFAFLKRPLLNPAGQNSSSVFVFFRTL
                     LELVRDRDPGYLVVTLDSRVPTFRHQRYTEYKANREKAPQDLHAQVPIIEEILAALGV
                     PCVRVDGFEADDVIATVAEGCRASRRPCWILSGDKDVLQLVGGNVRLLASERGSTELA
                     EYSREKVFETRGVYPEQIVDFLALTGDSSDNVPGVAGVGEKTAQKLLSQFGSLDAIYS
                     RLGEVAPESLRKKLEDGKESALLSRE"
     gene            complement(759..1202)
                     /locus_tag="VFH83_07715"
     CDS             complement(759..1202)
                     /locus_tag="VFH83_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgN"
                     /protein_id="HET6486290.1"
                     /translation="MEPSKESIAVLTRLREMLGRQREKFGRYLMLLEQEQISIETKDA
                     QRLLAQVEMERGLIDEIYALQKVIGPLEDLYASAYPRGEQTVPALRAVLDAMAAEIRE
                     RNQRNRTLLARRVEELRQEIAALHGWPRSRSPFVQPTPGFIDVRT"
     gene            complement(1234..1662)
                     /gene="fliS"
                     /locus_tag="VFH83_07720"
     CDS             complement(1234..1662)
                     /gene="fliS"
                     /locus_tag="VFH83_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HET6486291.1"
                     /translation="MNTNDSVQAYREIQIKTANQIRLIVMLYDGAIRHLNRSLDLFAD
                     GHRRFDELNTHIIAAQDILSELMASLDFDRGGALARNLFSLYAFMNRRLLEANLKKES
                     GPIAEVKKQLSDLREAWDELSTRKGLEERAGPAGGVNIAG"
     gene            complement(1680..>3885)
                     /locus_tag="VFH83_07725"
     CDS             complement(1680..>3885)
                     /locus_tag="VFH83_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET6486292.1"
                     /translation="SFSGLNLSPKDRVLFTASVTSPEAGPYDTLFLADAATRKLRQLT
                     FFPEQVLLLQDGDVLQIQNRFGVFRSSNGFTNIAPLPLFPSFVAGNEIAMGKLAPMAT
                     SPDGRYLMYLDRRSPAYGQLIMVDVAAGTTTVISDRVELDLQELPALWSPDSQFIVYA
                     KGGGLYYFSLAQLQQKRVLTEALRRMGDGTVASVRWAGARTLYYIDGTTVYSIDPSEL
                     STRALYTGFLDIGTVVGRIPFSFNANFDAFWISPDAGSLLLDVSGGNLLLAPLTNQAV
                     APRTMPYLWLPRGTTVASVLWSSQNVVTILCRTRDSGKLGSAVYRIAQDSQGHFGTFQ
                     QTADQGVLDLSLSPDGSLVALLRPDGVSWNDYTAWQEKGRVSRGKALHALWLSNDEIL
                     IAGSAVIERYTISTAASVLVAVSQPGPSGFTDADGVQMKLADQGLAFDEGAGGWSTTA
                     AFSVRDTGLTSDNYRVYLEDSTRGSYRNLVMVRDAKGFGTVPLFPLEATTYEAFPAVE
                     EKPDLANFSHGSRLRRREVSLVFNAADGAEGLTGILNTLSSYGIRATFFENGQFIRRN
                     PDAVVEIAASGHEVGSLFSSYFNLTDSRYAVDAAFIKAGLARTEDDYFATSGRELSLL
                     WHAPYYIVNSSIITAAATMNYVYVGRDLDTYDGVPDTEANRQQGLYKPAADLVERIIG
                     LKKPGSIIAVQIGTGMGQRSDYLFQKLDLLINELLRRGYSVVPVSTLIEHAR"
BASE COUNT          650 a         1244 c         1325 g          666 t
ORIGIN      
        1 gctcgcggga aagcagggcg gactccttcc cgtcctcgag cttctttcgc agggactcgg
       61 gagcgacctc ccccaatcgg gagtagatcg cgtccagaga gccgaactgg gagagaagct
      121 tctgcgccgt tttctcaccg acgcccgcta ctcccggaac gttgtccgag gagtctcccg
      181 taagcgccag gaagtccacg atctgctcgg gatagacccc gcgggtctcg aacacctttt
      241 cgcgggagta ttccgcaagc tccgtggagc cgcgctcgga ggccagcagg cggacgttgc
      301 cgcccacgag ctgcaggacg tccttgtctc ccgagaggat ccagcacggc cggcggctgg
      361 cgcggcatcc ctcggcaacg gttgcgatca cgtcatccgc ctcgaagccg tcgacgcgga
      421 cgcatggcac gccaagggcg gccaggatct cctcgatgat gggcacctgg gcgtgaaggt
      481 cctgtggcgc cttctcccgg ttggccttgt attcggtata gcgctggtgg cggaaggtgg
      541 gcacgcgcga gtcgagcgtc accacgaggt agccgggatc ccggtcgcgg accagctcca
      601 gcagcgtccg aaagaacacg aaaaccgagg aggagttctg tcccgccggg ttgagcagcg
      661 gacgcttgag gaaggcgaag tatccgcggt agatgacgct gtacccgtcg atgagataga
      721 ggtcctggct catgggcgat gatccccgag gtgccggctc acgtacggac gtcgatgaaa
      781 ccgggagtcg gctgaacgaa cggggatcga gaccgcggcc agccatggag ggcggcgatc
      841 tcctgtcgga gctcctctac cctgcgcgcc aggagcgtgc ggttcctctg gttgcgctca
      901 cggatctcgg cagccatcgc atccagcacc gcccgcaacg cgggtaccgt ctgttctccc
      961 cgcggatagg cggacgcata gaggtcttcc agcgggccga tcaccttctg gagggcgtag
     1021 atctcgtcga tcaggccacg ctccatttcc acctgggcca ggagccgctg ggcgtccttc
     1081 gtctcgatgg agatctgctc ctgctccagg agcatgagat agcgcccgaa cttttcccgc
     1141 tggcgcccca gcatctctcg caggcgcgtc aggacggcga tgctctcttt cgacggttcc
     1201 atggttcccc tccccggctc ggcgccgctg ccctcagccc gcgatgttca cgccgccggc
     1261 cggcccggcg cgctcctcca gccccttgcg cgtggagagc tcgtcccagg cctcccgcag
     1321 gtcgctgagc tgcttcttga cctcggcgat cggcccggac tccttcttca gattcgcttc
     1381 gaggagccgg cggttcatga aagcgtacaa actgaaaagg ttccgggcca gcgcgccacc
     1441 ccggtcgaag tccagggacg ccatcagctc ggacaggatg tcctgggcgg cgatgatgtg
     1501 ggtgttcagc tcgtcgaagc ggcggtgacc gtccgcgaac aggtccagcg agcggttcag
     1561 gtgtcggatg gccccgtcgt acagcatgac gatcagccgt atctggttgg ccgtcttgat
     1621 ctggatctct cggtatgcct gcaccgaatc gttggtgttc accccttccc cttccttcgc
     1681 taccgggcat gctcgatcag tgtggagacc ggaacgacac tgtagccccg gcgcagaagc
     1741 tcgttgatca gcaggtccag cttctggaac aggtagtcgc ttcgctgacc catccccgtg
     1801 ccgatctgca ccgcgatgat ggagccgggc ttcttcagcc cgatgatccg ctccaccagg
     1861 tccgccgccg gcttgtacag cccctgctgc cggttggcct cggtgtccgg caccccgtcg
     1921 taggtgtcca ggtcccgtcc cacgtagacg tagttcatgg ttgccgccgc cgtgatgatg
     1981 ctcgaattga cgatgtagta aggcgcgtgc cacagcaggg agagctcccg gccgctggtg
     2041 gcgaagtaat cgtcctcggt ccgcgccagg cccgccttga tgaaggcggc gtccaccgcg
     2101 tagcgggagt ccgtcagatt gaagtacgac gagaagaggg atcccacctc gtgtcccgag
     2161 gcggcgatct ccaccacggc gtccgggttg cggcggatga actgcccgtt ctcgaagaag
     2221 gtggcccgga tgccgtagga ggagagggtg ttgagaatcc cggtgaggcc ttccgctccg
     2281 tccgccgcgt tgaacacgag gctcacctcg cgccgccgca gtcgggagcc atgggagaag
     2341 ttcgcaaggt ccggcttctc ctccacggcg gggaacgctt cgtaggtggt cgcctccagc
     2401 gggaagagcg gcacggtgcc gaaccccttg gcgtcccgga ccatcaccag gttgcggtag
     2461 gagccgcggg ttgaatcctc caggtacacc cggtagttgt ccgaggtgag gccggtgtcc
     2521 cgcacgctga acgcggcggt ggtcgaccag ccaccggccc cctcgtcgaa tgccagcccc
     2581 tgatcggcga gcttcatctg gacgccgtcc gcgtcggtga acccggaagg cccgggctgc
     2641 gagaccgcga ccagcaccga agcggcggta gagatcgtgt accgctcgat gacggcggaa
     2701 ccggcgatca ggatctcatc gttggagagc cagagggcgt gaagcgcctt cccccggctg
     2761 acgcggccct tctcctgcca cgccgtgtaa tcgttccagc tcacgccatc gggccgaagg
     2821 agcgccacca ggctgccgtc cggcgagagc gacaggtcca gcaccccctg gtccgcggtc
     2881 tgctggaacg tcccgaagtg gccctgggag tcctgggcga tgcggtagac ggcggagccg
     2941 agcttgccgg agtccctcgt cctgcagagg atggtcacca cgttctggga cgaccagagc
     3001 accgatgcca cggtagtgcc ccgaggcagc cagaggtacg gcatggtccg cggcgccacg
     3061 gcctggttgg tgagcggagc caggaggagg ttgcctccac tcacatccag gagaaggctg
     3121 ccggcgtccg gtgagatcca gaacgcgtcg aagttggcgt tgaagctgaa cgggatcctt
     3181 cccaccaccg tcccgatgtc caggaagccg gtgtacagcg cgcgcgtgga gagctcgctg
     3241 gggtcgatgg agtacaccgt ggtcccgtcg atgtagtaga gcgtgcgcgc gccggcccac
     3301 cggacgctgg ccacggtgcc gtcccccatg cggcggagcg cctcggtgag cacccgtttc
     3361 tgctggagtt gagccaggga gaagtagtac aggccgccgc ccttggcgta tacgatgaac
     3421 tgggagtccg gggaccagag ggcgggcagc tcctggaggt cgagctccac gcggtccgag
     3481 atcaccgtgg tggtcccggc cgcgacgtcc accatgatga gctggccgta tgccggggag
     3541 cgtcggtcca ggtacatcag gtatcgtccg tcgggggacg tggccatggg ggcgagcttc
     3601 cccatggcga tctcgttccc ggcgacaaag gaagggaaaa gcggcagcgg ggcgatgttg
     3661 gtgaaaccgt tggaggaccg gaacactccg aagcggttct ggatctggag cacgtccccg
     3721 tcctggagca ggagcacctg ctcggggaag aacgtgagct ggcgaagctt gcgcgtcgcg
     3781 gcatccgcga ggaacagcgt gtcgtagggg ccggcttcgg gagaggtgac gctggcggtg
     3841 aacagaacgc ggtccttggg cgacaggttc agcccggaga aactg
//
LOCUS       DASRZS010000314         4921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3635747, whole genome shotgun sequence.
ACCESSION   DASRZS010000314 DASRZS010000000
VERSION     DASRZS010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3635747"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VFH83_07730"
     CDS             <1..518
                     /locus_tag="VFH83_07730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6486293.1"
                     /translation="AGPALERAIEFESVVRQEDINPVTGLPSFQELYSMLALELARLT
                     EQGGTLAVLIVELANYNDLAQRYGKPPTLGLLRDVARVIQEAAGSQARLFHYKGEAQL
                     AVICPKLDADGTSLLSLTLLERANSTEWTAGGERAHLELMLGFANRSRAAQTADSLLA
                     AAENLLEMQRV"
     gene            520..1263
                     /locus_tag="VFH83_07735"
     CDS             520..1263
                     /locus_tag="VFH83_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486294.1"
                     /translation="MPGFDFFRSRRLGYRPRTPAAALRAVETGTLPVEDFIYASTSAK
                     PLDEEPFDLEEIERLLSRQDMVLQTSLLLKRVLGKLTDSLEQETALFGAEGIAALEGR
                     ALEGAALIASRHPRERDSKTWKRLARKYYELSELHRDTGSVRNFYLGLAHDALQRGMA
                     GGEASVPDLALAVDILVSLGLHHQGTRLLEGSPDPRRPEILMLAARAAFHRGDYQGVS
                     DCCRALAPIRDSLSPEEQRVVSFWTQLDG"
     gene            1256..2695
                     /gene="pelF"
                     /locus_tag="VFH83_07740"
     CDS             1256..2695
                     /gene="pelF"
                     /locus_tag="VFH83_07740"
                     /inference="COORDINATES: protein motif:HMM:NF038011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HET6486295.1"
                     /translation="MADASRAAGSTPLTVCLLLEGSYPYITGGVSGWVQELIQALPDV
                     RFSLLTISPRAGLVKRYTLPQNVVAHRDVVLTERHASRGGPTGGSPAFLEAVRLLHAG
                     FASASAPDLEAVLSHMPVGYFPYTDAVREPAAWDMIVSSYQEHNPVYPFSEYFWSWKS
                     SHDMVFRALGEETPPADLYHAVCTGYAGITAVAAKLRTGRPFVLTEHGLYHKEREMEI
                     KRAQFVKGYQRDLWISMYAGISRLCYRHADLIVSLFEQNRRRQIALGAEEDKAIVIPN
                     GIDIPRYSAVVREKREGFHVGLVGRVVPIKDIKTFILMAQIVAAAIPEARFWCIGPMD
                     EDAAYSEECEALVRSLLLSDRFTFTGKTDVSAYYSFLDVLVLTSVREAQPLVILEAFA
                     VGLPVVSTRVGNVPELLDYQDRFLASPRDAGALAQAVKGIHDRPEDVAELAAKNRQKV
                     HRFYDKSRVFGQYGEIYRRLAAGGGPWRA"
     gene            2683..4122
                     /gene="pelG"
                     /locus_tag="VFH83_07745"
     CDS             2683..4122
                     /gene="pelG"
                     /locus_tag="VFH83_07745"
                     /inference="COORDINATES: protein motif:HMM:NF028243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide Pel transporter PelG"
                     /protein_id="HET6486296.1"
                     /translation="MAGIGFELRRVVRQGGALGFIGASLAGTVVVAGPWILSLLGIVL
                     IQRYAGLALSESPALFTSTIVYSSALSLVLFTGLHYVFSRQVSDLVYVHKDQEAGSAL
                     LAFLLGTIALAGVLAAAGVLPLRVAGTTSRPGLFLLGAGLLFVGTSANWVLMSFISLL
                     RSYVGIFLAYLAGSLASFAGTFLLGRAYGTGGALLGYSVGQWVIVIILYGMTLESYRP
                     AAVSLKGLMIYLRRYRLLFLAGALYAGATWVDKIVFWFAFGTRVPGTWWRLYDRYDVP
                     VFFSVLSLIPGLVYFTIETETAFYPRMREFLRSVSSDTFQRIQQRKYAMIRSLTTGLR
                     GQSVLQGIVSLVLILAAPTIGPFLFGPSLDVRALQVTLAAVFFHALFLSLLIFLFYLE
                     LYGLAALSGLAFLVVNLAASIAIVGISGAGISDAGNGDSRFLGVSYLLGAIAGCVVAG
                     LSLARQVRRLDHILFIRATLASPNDALRD"
     gene            4200..>4921
                     /locus_tag="VFH83_07750"
     CDS             4200..>4921
                     /locus_tag="VFH83_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486297.1"
                     /translation="MKKLIVPILAMILVLAMAGCNLMLGEKSSGTVSATDIAKFQRVF
                     MSSYTAERGGRSGGAKALSPFLPARATSSKAAVPVNLLTNLSFASLSPLTFSGYPEPG
                     QTTSFTAVVYDAANNVYDITATTTYAAGDNRKNYVEEYYIRDIGKNSSGFFDTATPDH
                     VWTVDDPIVSWTGAAWVQDQKARVRQVLTFTDGTTRTEHIVSQTDFTQASPSPKFAAF
                     DPNGSLSFGQLFIPATDTSAQFS"
BASE COUNT          757 a         1676 c         1579 g          909 t
ORIGIN      
        1 tggccggacc ggccctggaa cgggccatcg agttcgaaag cgtggtgcgc caggaggaca
       61 tcaacccggt cacgggtctc ccatcgttcc aggagctcta ctcgatgctg gccctggagt
      121 tggcgcgcct gacggagcaa ggaggcaccc tggcggtcct gatcgttgag ctggccaact
      181 acaacgatct ggcccagcgc tacggcaagc ccccgaccct gggcctcctt cgggacgtgg
      241 cccgcgtgat ccaggaggcg gccggatcgc aggcgcgcct cttccattat aagggcgagg
      301 cgcagctcgc ggtgatctgt cccaagctgg acgcggacgg cacctcgctc ctttcgctca
      361 ccctgttgga aagggccaac tccaccgagt ggaccgcggg cggggagcgg gcccacctgg
      421 agctcatgct gggtttcgcc aaccggtccc gcgcggccca gaccgcggac agcctcctgg
      481 ccgccgcgga gaacctcctc gagatgcaga gggtgtagcg tgccgggatt cgacttcttc
      541 cggtcccgcc gcctcggcta tcggccgcgc acgcccgcgg cggcgctccg cgcggtggag
      601 acggggaccc tgcccgtgga ggacttcatc tacgccagca cctccgccaa gccgctggac
      661 gaggaaccct tcgacctgga agagatcgag cgcctcctct cccgacagga catggtcctt
      721 caaaccagcc tcctcctgaa acgggtcctg ggcaagctca ccgacagtct ggagcaggag
      781 accgccctct tcggagccga ggggatcgcg gcgctggagg gccgggcgtt ggaaggcgcg
      841 gccctgatcg cctcccgaca cccgcgagag cgcgatagca agacctggaa gcggctggcg
      901 cggaagtact acgagctgtc cgagctgcat cgggacacgg gctccgttcg aaacttctac
      961 ctgggcctgg ctcatgacgc gctccagcgg gggatggccg gcggggaggc gtcggtgccc
     1021 gaccttgcat tggccgtgga tatcctggtg tccctggggc tccaccatca gggaaccagg
     1081 ctgctcgaag gatcgccgga cccgcgccgg ccggagatcc tcatgctggc tgcccgcgcg
     1141 gcgttccacc gcggagacta ccagggcgtg tccgactgct gtcgagccct ggcgcccatc
     1201 cgagacagcc tctcccccga ggaacagagg gtggtctcct tctggacgca gctcgatggc
     1261 tgacgcatcc cgggcggcag gctccactcc cctcaccgtg tgtctcctgc tggaggggtc
     1321 gtacccgtac attacgggcg gcgtctccgg ttgggtacag gagttgatcc aggccctccc
     1381 cgatgtccgc ttctcgctcc tcacgatctc tccccgcgcc gggctggtga agcgttacac
     1441 cctgccgcag aacgtggtgg cccaccggga cgtcgtgctc acggagaggc atgcatcccg
     1501 cggcggcccc accggcgggt ccccggcgtt cctggaagcg gtgcggctcc tccacgccgg
     1561 cttcgcctcc gcatccgcgc cggacctcga ggcggtcctc tcccacatgc cggtcggcta
     1621 cttcccgtac acggacgccg tgcgcgagcc ggcggcgtgg gacatgatcg tctcctccta
     1681 ccaggagcac aacccggtgt atccgttctc cgagtacttc tggtcctgga agtcctccca
     1741 cgacatggtg ttccgcgcgc tgggggagga gacgccgccc gcggacctct accacgccgt
     1801 ctgcaccggc tacgccggga tcacggcagt cgcggccaag cttcgaacgg gtcggccgtt
     1861 cgtcctgacg gagcacggtc tgtaccacaa ggaacgggag atggagatca agcgcgccca
     1921 gttcgtgaag ggctaccagc gcgatctgtg gatctccatg tacgccggca tcagcaggtt
     1981 gtgctaccgc cacgcggacc tcatcgtctc tctcttcgag cagaaccgcc ggcgccagat
     2041 cgcgctgggt gccgaggagg acaaggccat cgtcatccca aacgggatcg acatcccccg
     2101 gtactcggcc gtggtccgcg agaaacgcga gggcttccac gtgggcctgg tgggacgggt
     2161 ggtccccatc aaggacatca agacgttcat cctgatggcc cagatcgtgg ccgccgccat
     2221 cccggaggcc cgcttctggt gcatcggccc gatggacgag gatgcggcct actcggagga
     2281 gtgcgaggcc ctggtccgca gcctgctgct ctccgaccgc ttcacgttca ccggcaagac
     2341 ggacgtgagc gcgtactact cctttctgga tgtgctggtg ctcaccagcg ttcgggaggc
     2401 gcagcccctg gtcatcctgg aggccttcgc ggtggggctg cccgtggtat cgacccgggt
     2461 gggcaacgtg cccgagctcc tggattacca ggatcggttc cttgcctctc cgcgagacgc
     2521 cggcgcgctg gcccaggcgg tcaagggcat ccacgaccgg cccgaggacg tggcggagct
     2581 ggcagcaaag aaccgacaga aggtgcatcg cttctacgac aagagccggg ttttcggcca
     2641 gtacggggag atctaccgca ggctcgccgc cgggggtggc ccatggcggg cataggcttc
     2701 gagctgcgga gggtggtgcg ccagggcggc gcgctcgggt tcatcggcgc gagccttgcc
     2761 ggcaccgtcg tggtggcagg gccctggatc ctgtccctcc tggggatcgt cctgatccag
     2821 cgatacgcgg gcctggctct ctcagagtcc ccggctctct tcacctctac catcgtctac
     2881 tccagcgccc tctcgctcgt gcttttcacc ggcctgcact acgtgttctc ccggcaggtc
     2941 tcggacctcg tctacgttca caaggaccag gaggccggct ccgcgcttct cgccttcctc
     3001 ctgggaacca tcgcgctggc gggcgtcctg gccgcggccg gggttctccc tctccgcgta
     3061 gcggggacca cctcccgtcc ggggctgttc cttctgggcg ccgggctcct ctttgtcggg
     3121 acctcggcca actgggtcct gatgagcttc atctccttgc tgcgctctta cgtcggcatt
     3181 ttcctggctt acctggccgg ctcgctcgcc tcctttgccg gtacgttcct cctgggcaga
     3241 gcttacggca cgggcggcgc gctcctggga tactccgtcg ggcagtgggt gatcgtgatc
     3301 atcctgtacg gcatgacgct ggaatcctac cggcctgctg cggtaagtct gaaaggcctg
     3361 atgatctacc tgaggcgcta ccgactgctc ttcctggcgg gggccctgta cgcgggtgcc
     3421 acgtgggtgg acaagatcgt cttctggttc gctttcggga ctcgggtgcc agggacgtgg
     3481 tggcgtctgt acgaccgcta tgacgtgccg gtcttcttct ccgtgctctc gctgattccc
     3541 gggctcgtct atttcaccat cgaaaccgag acggcattct atccccgcat gcgcgagttc
     3601 ctgagaagcg tatcatcgga cacgttccag aggatccagc agcggaagta cgcgatgatc
     3661 cgaagcctca ccaccgggct ccgagggcag agcgtgctcc aggggatcgt cagcctggtc
     3721 ctcatcctgg ccgctccgac gatcggcccg ttcctcttcg gaccgtctct ggacgtgcgg
     3781 gcgctccagg tgacgctggc ggccgtgttc ttccatgccc tgttcctgag tctgctgatc
     3841 ttcctcttct acctggagct gtacgggctt gccgcgcttt ccggccttgc gttcctggta
     3901 gtgaacctgg cagccagcat cgcgatcgtc ggtatctccg gggccggcat ttccgatgcc
     3961 ggcaatggag attctcgatt ccttggagta agctatctcc ttggagcgat agcgggctgc
     4021 gtcgtggcgg ggctttcgct ggcccgacag gtgagacggc tcgaccacat cctcttcatc
     4081 cgcgcgacgc tggcctcgcc gaacgacgcg ttgcgcgact gagatctcgc ggaaatcccg
     4141 gacatcccga actatactct attggtgcgc ctgagttctc acgcaccagg gggtttacga
     4201 tgaaaaagct gatcgtgcca atactggcga tgattctggt tctcgcgatg gcggggtgca
     4261 acctcatgct cggggagaag tcctccggaa cggtgagcgc cacggacatc gcgaagttcc
     4321 agcgggtgtt catgtcctcg tacacggcgg agcgcggagg acgctcgggg ggagcaaagg
     4381 cgctgagccc gttccttccc gctcgggcga ccagctccaa ggctgcggtg ccggtcaacc
     4441 tgctgacgaa cctctccttc gcctcgctca gcccgctgac cttcagcggg tatcccgagc
     4501 ccggccagac caccagcttc acggccgtgg tctacgacgc cgccaacaac gtctatgaca
     4561 tcaccgcaac cacgacgtat gccgcgggag acaatcgcaa gaactacgtg gaggagtact
     4621 acatccggga catcggcaag aactcctcgg ggttcttcga cacggcaacg ccggaccacg
     4681 tctggacggt cgatgatccc atcgtaagct ggacgggcgc ggcttgggtc caggaccaga
     4741 aggcgcgcgt gaggcaggtc ctgacgttca cggacgggac cacccggacc gagcacatcg
     4801 tctcccagac ggacttcacg caggccagtc cctctccgaa gttcgcggcc ttcgatccga
     4861 acggctccct gtccttcggg cagctcttca tcccggccac ggataccagc gcccagttct
     4921 c
//
LOCUS       DASRZS010000315         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_563101, whole genome shotgun sequence.
ACCESSION   DASRZS010000315 DASRZS010000000
VERSION     DASRZS010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563101"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFH83_07755"
     CDS             <1..441
                     /locus_tag="VFH83_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HET6486298.1"
                     /translation="TKVEMFVYCHPDASDAMLDRLAGIEERIYQALEIPYRIVDTCTG
                     DLGAPAYRKYDFEAWMPGRGESGEWGEITSASNCTDYQARRLGVRFKEAGKNQFVHTL
                     NGTAVAISRTLIALLENNQQPDGSIRLPSALSAYTGFSEIPARG"
     gene            complement(480..698)
                     /locus_tag="VFH83_07760"
     CDS             complement(480..698)
                     /locus_tag="VFH83_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486299.1"
                     /translation="MTSIVCDSCKKVVQGARKDQNFSVFMEKDICIPCREELEDATKR
                     QMLSRRPYTLKDYWTTYNTTLTRMCSRG"
     gene            824..1924
                     /locus_tag="VFH83_07765"
     CDS             824..1924
                     /locus_tag="VFH83_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HET6486300.1"
                     /translation="MSDVLQRLKMENIPYLLCGFSSLDRFFRVKEPGPLYVATDSSIV
                     SLAKAFDGLQFPGLPLEDAALHTDDVRLVFRCVDSLAVPPTAPFTVMRLLYDPDRNAF
                     IDRLELYPDLRASTLSLIPDSSPKWVGLCEAARLVSRYHYDAEGLPIDWCRGDALPPI
                     PYQRDLLVGLLTSRSPEKGLALLAEAGFVEEAWPELAAMARIPHAKDYHPEGNVWEHT
                     LATLKYRKRPDLVLSLSLLLHDSGKPTAENVGDRRFDRHAEIGARLASRFLTRLGFHP
                     KTIEAVEFLVLYHMMPPALKTLPPFRTEPVFSSPLFPTLLELYRADASSSYAGEEGYY
                     EACRIYRAWQKERDNPYRGQKQHHQRSKAAKW"
     gene            complement(1980..>2830)
                     /gene="thrC"
                     /locus_tag="VFH83_07770"
     CDS             complement(1980..>2830)
                     /gene="thrC"
                     /locus_tag="VFH83_07770"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET6486301.1"
                     /translation="KAFHRKRGIDVVILFPSGRVSALQERQLTTLGDNVHALEVKGSF
                     DDCQRLVKEAFRDPVLKARLRLTSANSINVGRLIPQALYYIYAGVRSPARPPVFCVPS
                     GNFGNLTAGVYAWYWGLPVAGFVAATNVNDVVPEYLRTGLFSPRESLATLSNAMDVGN
                     PSNFERLLALFGEDRDAMARMIRGAVVSDERTLAAIRDVHRRHGILVDPHTAVGIAAA
                     EPGRPTIVLSTAHPGKFADTVRRATGVEPQLPERLARCLSLPSHADPVGTALPELSRY
                     LLERFG"
BASE COUNT          484 a          916 c          932 g          498 t
ORIGIN      
        1 accaaggtcg agatgttcgt ctactgccac ccggatgcct cggatgccat gctggatcgg
       61 ctggccggga tcgaggagag gatctatcag gcgctggaga tcccctaccg gatcgtggac
      121 acctgcaccg gagacctggg ggcgcctgcg tatcggaagt acgacttcga agcctggatg
      181 cccgggcggg gcgaatccgg ggaatggggc gagatcacca gcgcgtccaa ctgcaccgac
      241 taccaggcgc gccggttggg ggtccgattc aaggaagcgg ggaagaacca gttcgtgcac
      301 acgctcaacg gaaccgcggt ggccatctcc cggacgctca tcgcgctcct ggagaacaac
      361 cagcaaccgg acggatccat ccggcttccg tcagccctga gcgcgtacac cgggttctcg
      421 gagatcccag ccagaggctg atcggcccct ggccaagggg cggggtcagg ccgagcctgt
      481 cagcccctgg agcacatccg cgtaagcgtg gtgttgtacg tcgtccagta gtccttgagc
      541 gtataggggc gacgggagag catctgccgc ttggtggcat cctccagctc ttcccggcag
      601 gggatgcaga tgtccttctc catgaagacc gagaagttct ggtccttgcg cgcaccttgc
      661 acgaccttct tgcagctatc acacacgatc gatgtcattt cggtgtctcc tccgagaatg
      721 tagcgtgcat agtaggtgat ttccatccgg ttgtcgagtg gacgggtggt ggcaaagtgt
      781 gtaccataag gggtgccggt acccccgggg gaaggggtct ctcatgagcg atgtgctgca
      841 gcgattgaaa atggagaaca tcccttacct cctgtgcggc ttttcctccc tggatcggtt
      901 ctttcgggtc aaggagcccg gtccgctcta cgtagctacg gattcttcca tcgtatccct
      961 tgccaaggcg ttcgatggcc tgcagtttcc aggtcttccc ctggaggacg ccgcgctgca
     1021 tacggatgac gtacgcctgg tgttccgctg cgtggactcc ctggcggttc ccccgacggc
     1081 gcctttcacg gtgatgcggc tcctctacga cccggaccga aacgccttca tcgaccgtct
     1141 ggagctctac ccggaccttc gggcgtccac cctcagcctc atccccgact cctcccccaa
     1201 gtgggtaggc ctgtgtgagg cggcccgcct ggtttcccgc taccattacg atgcggaggg
     1261 attgccgatc gactggtgcc ggggcgatgc tctcccgccc attccgtatc agcgggacct
     1321 cctggtgggt ctcctgacgt cccgctcccc cgagaagggt ctggcgctgc tggcggaggc
     1381 cggcttcgtg gaggaggcgt ggccggagct cgcggccatg gccaggatcc cccacgccaa
     1441 ggactaccac cccgagggga acgtgtggga gcacacgctg gccacgctca agtaccgaaa
     1501 gcgcccggac ctcgtgcttt ccctttcgct gctgctgcac gacagcggaa agcccaccgc
     1561 ggagaacgtt ggggaccgga ggttcgaccg tcacgcggag ataggggcgc gcctggcgag
     1621 ccggttcctg acgcggctgg ggttccatcc caagaccatc gaggcggtgg agttcctggt
     1681 tctctaccat atgatgccgc ccgccctgaa gaccctgccc ccgtttcgga ccgagccggt
     1741 cttctcctct ccgctgtttc cgaccctcct ggagctgtac cgagcggacg cgagctcgtc
     1801 ctacgccggg gaagaagggt attacgaggc gtgccgcatc taccgcgcct ggcagaagga
     1861 gcgggacaac ccctaccgcg gacagaagca gcaccaccag cggtcgaaag cggcgaagtg
     1921 gtgaaggccg tcacgctctt cgtagcggcg aggtgataga ggcgccggtc gagccgggcc
     1981 taaccgaacc gttccagcag atagcgcgaa agctcgggca gcgcggtccc gacgggatcc
     2041 gcatgcgatg gcagggacag gcaccgggcc agccgctcgg ggagctgcgg ctctacgccg
     2101 gtcgcgcggc ggaccgtgtc cgcgaacttc ccgggatgcg ccgtggaaag cacgatggtg
     2161 ggtcgtccgg gttccgccgc cgcgattccc accgccgtgt gcgggtccac caggatcccg
     2221 tggcgccgat ggacgtcgcg gatcgcggca agcgtgcgct catcgctcac cacggcccct
     2281 cggatcatcc gagccatggc atcgcggtcc tcaccgaaga gagcgagcag ccgctcgaag
     2341 ttgcttgggt tgcccacgtc catggcgttg gaaagcgttg ccagggactc ccgaggtgag
     2401 aagagaccgg tgcgcaggta ctcaggcacc acgtcattca cgttggtggc ggcaacgaag
     2461 ccggccaccg gcaggcccca ataccacgcg tagaccccgg cggtcaggtt gccgaagttg
     2521 ccgctgggga cgcagaagac cggagggcgc gctggcgatc gaactcccgc gtagatgtag
     2581 tacagcgcct gcgggatcag ccttcccacg ttgatggagt tggccgacgt cagccgtaag
     2641 cgcgccttca ggaccgggtc ccgaaacgcc tccttcacca gccgctggca gtcatcgaag
     2701 ctccccttca cctccagggc gtgcacgttg tcgccgaggg tggtgagctg gcgctcctgg
     2761 agggcgctca cccgccccga ggggaagagg atcaccacgt cgatgccgcg cttgcggtga
     2821 aaggccttgg
//
LOCUS       DASRZS010000316         5421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2868421, whole genome shotgun sequence.
ACCESSION   DASRZS010000316 DASRZS010000000
VERSION     DASRZS010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2868421"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="VFH83_07775"
     CDS             <1..1158
                     /locus_tag="VFH83_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET6486302.1"
                     /translation="KRAAKQPARLAAKAVGAEASVLNTNRAPEHAPRKRAATKAPARA
                     TGTLPMGAEDALRLYRTMFRIRRFETTCNELYLGAKIPGMSPHLYIGEEASAASVCFF
                     LNKDDYIVSNHRGHGHCLGKGAAMDRMLAEIMGKSTGYCRGRGGSMHIADVTLGNLGA
                     NGIVGAGIPIAVGAGLSIVYRGTRQIAVVFFGDAATNQGTFHEGVNMAGAMKLPVLLV
                     CENNQYGLSTLIQRTSSTAVLSDKAHGYGIEHVTVDGMDAEVAFQAARTAVEYVRAER
                     KPYFLEYDTYRFLGHGASDNRSYRTKEEEERWKKRSPVENFRLRMLSAYGIAEEKIKG
                     LEAEVETELANALKFAQESPEPSPDGALEDVFFPTPAVEQALPSASRGGKA"
     gene            1155..2132
                     /locus_tag="VFH83_07780"
     CDS             1155..2132
                     /locus_tag="VFH83_07780"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET6486303.1"
                     /translation="MSKMTYREAIRLALKEEMKRDERVFLLGEDVGKFGGAMAVTKGL
                     FDEFGARRVIDTPISEAAIVGAALGAALTGLLPVAEIMFIDFTGVCLDQIVNQVAKVR
                     YMLGGQVQVPLVIRTQGGAGKGYAAQHSQSLESVFSFYPGLIVVMPATPRDARGLLKS
                     AIREPNPVLFIEHKVLYNSEGEVPEAEELLPLGVAEVKRPGKDVTVVSHSMQLLKSLE
                     AAETLAKDHGVEAEVIDLRTVAPLDERTILASVRKTGHLVTVEEGYQNNNVGAEVISR
                     VVSKAFDALDAEPVRVSFPNCPVPFAKSLENALLPDPKKIVQACLTAVR"
     gene            complement(2223..3008)
                     /gene="ccsB"
                     /locus_tag="VFH83_07785"
     CDS             complement(2223..3008)
                     /gene="ccsB"
                     /locus_tag="VFH83_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HET6486304.1"
                     /translation="MATVALVLIAAAAALQAAFLLRRENRRDPISHWILAAAGLLLAA
                     TIVDRSIRIHFVAVTNMYESLVFFSGAVALVIMGLRLARRTRDLSPFVVFGATILCLA
                     LLAVSSSPLAPSGIQPPIPALQSYWLVLHVTFSFIGEAFFVVSFVAAIAFLAARDEDR
                     REALDRLVATAIGIGYPIFTAGALVFGAIWAQTAWGSWWSWDPKETWALITWLLYTAY
                     LHTRLVRRLRGRVAAILAIVGFVATVFTFFGVNFLLAGLHSYG"
     gene            complement(3008..4009)
                     /locus_tag="VFH83_07790"
     CDS             complement(3008..4009)
                     /locus_tag="VFH83_07790"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HET6486305.1"
                     /translation="MGAVYRFLKSVRLAVVLSLILTVLLLVSTLVPQGQEQAYYQARY
                     SPVVYGLVEMLGFDRFFTSALFLLPALLFSVNLGVCAVDRVVRRTAAHARLRVGPDLI
                     HIGLLVLIAGGLTTALGRQETELSLGVGDTAAVPPGYTLQLVDFQYLTYPDGSPRDWK
                     STVSVSRDGRELIASFPIEVNHPLRLRGVRIYQATWGIEGSLQLRDQKGVSVTATTGQ
                     GFQDGDSFWMFSQVEKTPDSAHPWRAVFDEYRGQETQRSGLYTDSPASTRTVGAGETI
                     GPYTVDAVTAREITGLKAVKDPGAVPFLVAAVLIVAGLALTFIQKRLDERGASEENA"
     gene            4067..4843
                     /locus_tag="VFH83_07795"
     CDS             4067..4843
                     /locus_tag="VFH83_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET6486306.1"
                     /translation="MKDRAERIMDILARRGYQSVSELAAELQVSDMTVRRYLDQLEKR
                     ELIKRTHGGAFAGQEMIEVDYRIRETVHREVKEGIGAKAFSLIQPGESVFIDAGTTTA
                     LLAYAITDTKRITVVTHSLVVARALENRTNVQCLLLGGTVHGATHSVLGPLAEEAISQ
                     FRFNRAFLGTSAIDLNEGLSQSTFEEIPVKRKAASQAQQVVVLADSSKFDKHVTFLFM
                     KLEEVNTIITDPGLSDTLAQGIRERGIELTIAAPTEGRGT"
     gene            complement(4848..>5421)
                     /locus_tag="VFH83_07800"
     CDS             complement(4848..>5421)
                     /locus_tag="VFH83_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6486307.1"
                     /translation="NGVQPDLATFAKIVGGGMPGAAVAGRAEILRLAGRGGGVRFSGG
                     TYSCHPASMLASRLMMEHLIAQEGEIYPRIALLGEKARRGAEEAFRAQGLPARCTGDP
                     GEALGGSSMGAVHFPFDASAPCDTPEQTRNPELCDVELSDTVVQLALLLEDVFVMHGM
                     GSVSAAHTEADVAQYAAACGRAARRIANSL"
BASE COUNT         1018 a         1790 c         1836 g          777 t
ORIGIN      
        1 aagcgcgccg caaagcagcc ggcgcgcctg gctgccaagg cggtcggcgc agaggcttcc
       61 gtcctcaaca ccaaccgggc gccggagcac gccccgcgca aacgcgccgc gaccaaagcc
      121 cccgcgcgag caaccgggac ccttccgatg ggcgcggagg acgccctgcg gctctatcga
      181 accatgttcc gaatccgcag gttcgagacg acctgcaacg agctatacct cggcgccaag
      241 atccccggca tgtccccgca cctgtacatc ggagaggagg cgtccgctgc cagcgtctgc
      301 ttcttcctga acaaggacga ctacatcgtg agcaaccacc gcggccacgg ccactgtctg
      361 ggcaagggcg cggccatgga ccggatgctc gcggagatca tggggaaaag taccgggtac
      421 tgcaggggtc ggggcggctc catgcacatc gcggacgtca ccctcgggaa cctgggggcg
      481 aacggcatcg tcggtgcggg gatcccgatc gccgttgggg cgggactctc tatcgtatac
      541 cgtgggaccc gtcagatcgc cgtggtcttc ttcggggacg cggctaccaa ccagggaacg
      601 ttccacgaag gcgtcaacat ggcgggagcc atgaagctgc ccgtgctcct ggtctgtgaa
      661 aacaaccagt atgggctttc caccctgatc cagaggacca gctccacggc ggtgctgagc
      721 gacaaggcgc acgggtacgg gatcgagcac gtgaccgtgg acggcatgga cgcggaggtg
      781 gcgttccagg ctgcgcggac cgccgtggag tacgtccgcg cggagcgcaa accgtacttc
      841 ctggaatacg atacctatcg gttcctgggc cacggagcca gtgacaaccg cagctaccgc
      901 accaaggagg aagaggagcg gtggaagaag cgatcccccg tggagaactt ccggctgcgg
      961 atgctctccg cctatgggat cgctgaggag aagatcaagg gcctggaagc ggaggtggaa
     1021 acggagctgg ccaacgcgct gaagttcgcg caggagagcc ccgagccgtc gccggacggg
     1081 gcgctggagg acgtcttctt ccccacgccg gccgtggagc aggccttgcc cagcgcaagc
     1141 cgcggaggca aggcatgagc aagatgacct atcgcgaagc catccgtctg gccctgaagg
     1201 aggagatgaa gcgcgacgag cgcgtgttcc tcctgggaga ggacgtgggc aagttcggcg
     1261 gtgccatggc ggtcaccaag ggcctgttcg acgagttcgg cgcgcgacgg gtgatcgaca
     1321 cgccgatctc ggaggccgcc atcgtcggcg ccgccctggg tgcggccctg accggcctcc
     1381 tgcccgtcgc ggagatcatg ttcatcgact tcacgggtgt ctgtctcgac cagatcgtga
     1441 accaggtggc caaggtacgc tacatgctgg gcggccaggt ccaggtgcct ctggtgatcc
     1501 gcacccaggg cggcgccggc aaagggtacg cggcgcagca ctcccagagc ctggagagcg
     1561 tgttcagctt ctaccccgga ctgatcgtag tcatgccggc cacgccccga gacgctcgcg
     1621 gtctgctcaa gagcgccatc cgcgagccca accccgtgct cttcatcgag cataaggttc
     1681 tgtacaactc ggaaggcgag gtgccggagg cggaggagct gctccccctg ggggttgccg
     1741 aggtgaagcg cccgggcaag gacgtcacgg tggtcagcca ctccatgcag ctcctcaagt
     1801 ccctggaggc cgcggagacc ctggccaaag accacggggt cgaggcggag gtgatcgacc
     1861 tgcggaccgt ggctcctctg gacgaaagga ccatcctggc atccgtgcgg aagaccgggc
     1921 acctggtgac cgtcgaggaa ggctaccaga acaacaacgt gggcgcggag gtgatctctc
     1981 gagtggtctc caaggcgttc gacgcgctgg atgcggagcc tgtccgcgtt tccttcccga
     2041 actgccccgt gccgttcgcg aagtccctgg aaaacgcgct tcttcccgat ccgaagaaga
     2101 tcgtgcaggc atgcctcacc gcggtgaggt aggggccggc cgcgcgccga cccggccgac
     2161 cgggcactcc ggggcgtcgc cgcggggcgc cgccgcgggg cgccgtcgcg ggggcgacgc
     2221 ctctatccgt agctgtgcag gcccgcgagc aggaagttca cgccgaagaa cgtaaacacc
     2281 gtggccacaa acccgacgat ggccagaatg gccgccaccc tgccccgaag tcttcggaca
     2341 aggcgggtgt gcaggtacgc ggtgtaaagc agccaggtga tgagcgccca ggtctccttg
     2401 gggtcccagc tccaccagct tccccaggcc gtctgcgccc agatggcgcc gaacaccagc
     2461 gcgcccgcgg tgaagatcgg gtacccgatt ccgatcgcgg tcgccaccaa ccggtccagg
     2521 gcctctcgcc ggtcttcgtc ccgagccgca agaaacgcga tggcggcaac aaagctcacc
     2581 acgaagaacg cctcgccgat gaaggagaac gtcacgtgga gaaccagcca gtaggactgg
     2641 agggccggaa tgggcggctg gatcccgctc ggcgcgagcg gggaagagga gaccgccagc
     2701 agcgcgaggc agaggatggt cgcgccgaag acgacgaagg gcgacaggtc ccgggtcctg
     2761 cgggccaggc gcagacccat gatgacgagc gccacggctc cggagaagaa caccagggac
     2821 tcgtacatgt tggtgaccgc gacgaaatgg atccggatgc tgcggtccac gatggtggcc
     2881 gccaggagca ggcccgcggc cgccaggatc caatgggaga tggggtcccg acggttctcc
     2941 cttcgaagca ggaacgcggc ttgcagcgcc gcggcggcgg cgatgagcac cagggcgacg
     3001 gttgccatca ggcgttctcc tccgaagccc cgcgctcgtc caaccttttc tggatgaacg
     3061 tcagcgccag gcctgcgacg atcagtacgg ccgccaccag gaacggcacc gcaccggggt
     3121 ccttcacggc cttgaggccg gtgatctccc gcgccgtaac ggcgtctacg gtgtagggtc
     3181 cgatggtctc cccggccccc accgtccggg tggaagccgg cgagtcggtg tagagccccg
     3241 acctttgcgt ttcctggcct cgatactcgt cgaagacggc gcgccaggga tgcgccgaat
     3301 cgggggtctt ttccacctgg gagaacatcc agaacgaatc accgtcctgg aacccttgcc
     3361 cggtggttgc cgtaacgctc acgcccttct ggtcgcgaag ctgcagactg ccctcgattc
     3421 cccaggtggc ctggtaaatc ctcacgcccc gcagacgcag cggatgattg acctcgatgg
     3481 gaaacgatgc gatcagctcc cgtccgtccc ggctgacgct cacggtggat ttccagtctc
     3541 tagggctgcc gtccgggtag gtaaggtact ggaagtccac gagctgaagc gtgtagcccg
     3601 ggggcaccgc cgccgtgtcc cccaccccca gagaaagctc cgtctcctga cggcccaggg
     3661 cggtcgtcag tcccccggcg atcagcacga gcaggccgat atggatgagg tccggcccca
     3721 cgcgcagcct ggcgtgagcc gccgtccgcc ggaccacgcg gtccacggcg caaacaccca
     3781 ggttgacgct gaaaaggagc gcgggaagca ggaacagggc ggaggtgaaa aaacggtcga
     3841 acccgagcat ctccaccagg ccgtagacca ccggcgaata ccgcgcctgg tagtacgcct
     3901 gctcttgccc ctgggggacc agcgtcgaga ccagcagaag gaccgtgagg atcaagctca
     3961 ggaccaccgc cagccggacg gacttgagga agcgatagac cgcacccacg gtcagactat
     4021 atcgcatccc gcccagaagg attagagtcg cggcaggagg cccagggtga aggaccgtgc
     4081 ggagaggatc atggacatcc tggcgcgcag gggctaccag agcgtgagcg agctcgcggc
     4141 ggagctccag gtatccgaca tgacggttcg ccggtacctg gaccagctcg agaagaggga
     4201 gctcatcaag cggacgcacg gcggcgcctt cgcggggcag gagatgatcg aggtggacta
     4261 ccgcatccgg gaaacggtcc accgtgaggt caaggagggg atcggggcca aggcgttctc
     4321 cctgattcag ccgggggaat cggtgttcat cgacgcaggg accaccacgg ccctgctggc
     4381 ctacgccatc accgacacca agaggatcac cgttgtgacg cattccctgg tggtggcgcg
     4441 agcgctggaa aaccgtacca acgtgcagtg cctcctcctg ggcggcaccg tccacggggc
     4501 aacgcactcg gtcctgggcc cgctggcgga ggaagccatc tcccagttcc ggttcaaccg
     4561 cgccttcctg ggcaccagcg ccatcgatct gaacgaggga ctctcccaga gcaccttcga
     4621 ggagatcccg gtgaagcgaa aggcggcgag ccaggcccag caggtggtcg tcctcgcgga
     4681 cagctccaag ttcgacaagc acgtgacgtt cctcttcatg aagctcgaag aggtgaacac
     4741 catcatcacg gatccgggcc tgagcgacac cctggcgcag gggatccgtg agcgcggcat
     4801 cgagctcacg atcgccgctc ccaccgaggg gcgcggaacg tagtcgtcta cagggagttg
     4861 gcgatccgcc gggcggcgcg gccgcacgcg gccgcgtact gggcgacatc cgcctccgta
     4921 tgggcagcgc tcacggaccc catcccgtgc attacgaaca cgtcctccag caggagcgcg
     4981 agctgcacga ccgtgtcgga cagctccacg tcgcagagct cggggttgcg cgtctgctcc
     5041 ggcgtgtcgc agggggccga ggcgtcgaac gggaaatgga ccgcgcccat cgagcttccg
     5101 ccaagggctt cccccgggtc tccggtacag cgcgcgggga gcccctgggc gcggaaagcc
     5161 tcctcggcgc ctcggcgggc cttctccccc aggagcgcga tgcgcggata tatctcgcct
     5221 tcctgggcaa tcaggtgctc catcatcagg cgggaggcga gcatcgatgc ggggtggcag
     5281 gagtacgtgc ctccggagaa gcgcacaccc ccgccccttc cggccagccg caggatctcc
     5341 gctctgcccg caaccgcggc ccccggcatg ccgccaccca cgatcttggc gaaggtagcc
     5401 aggtccggct gaaccccgtt c
//
LOCUS       DASRZS010000317         4940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4408662, whole genome shotgun sequence.
ACCESSION   DASRZS010000317 DASRZS010000000
VERSION     DASRZS010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4408662"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFH83_07805"
     CDS             <1..186
                     /locus_tag="VFH83_07805"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6486308.1"
                     /translation="QIESLYSQGLVEYANHNYQKAIDLWEQVLVLDPHYQPAAEYIAT
                     AKKTLMLQEDLTNRNPQ"
     gene            194..1339
                     /locus_tag="VFH83_07810"
     CDS             194..1339
                     /locus_tag="VFH83_07810"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET6486309.1"
                     /translation="MEANETPDTLSHPIPEQTLRRIPQYHQILAEMQGRGEAYVSSRH
                     LAQFFRIDDTQVRKDVSLIGYKGKPKAGYSILGLKRAIEEFLGINYENTAILIGAGRL
                     GSALSQYPGLAEYGLKLVAIFDNDSAKTGSVLGAFTILPMESLQRVVRSFDVGIAIVC
                     VPKGAAQAVAGRVVSLGIKAIWNFSPTQLEVPSDVVVRNENIALGLAILSHYVKRQKR
                     DGQSPPAPRENPPQRLIDIFSRYQPKREYLVRILEETQKGYGYLPRDAVGHIADFLGL
                     TREKVQETAAFYPVFRMEPPARFRIVVCNGENCVRKGSRDLVESVARELKIGVGETSA
                     DGAFSLETVPCRGLCSTAPNVTINDKLVTVHTPEELLEHLRQLNPGR"
     gene            1409..2281
                     /gene="mazG"
                     /locus_tag="VFH83_07815"
     CDS             1409..2281
                     /gene="mazG"
                     /locus_tag="VFH83_07815"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET6486310.1"
                     /translation="METLPQDAFLRLLGIVQRLRAPNGCPWDLEQTPSTLRASVVEEA
                     WECVSAIDSGDEENLAEELGDLYLMATMVSWMKEQEGTFSVSAVLQGISDKLVRRHPH
                     VFGEAVASTPEAVLEQWAAIKATEKPRPTSQTSALDGVPGSLPPLERAFEVQKKAAKV
                     GFDWPGPKPVWEKLEEETAELREARESGSDAAVESELGDLLFTVVNLSRLLGVDPSVA
                     LNRANDKFSRRFRQVEDRLRAQGSAPKEAGLERMDAIWNQVKAEEAGSQPLAASGDAP
                     EDTGGSPATPSTSK"
     gene            complement(2206..4542)
                     /locus_tag="VFH83_07820"
     CDS             complement(2206..4542)
                     /locus_tag="VFH83_07820"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HET6486311.1"
                     /translation="MATTRLPARLTSLLRRVDRPGRYVGGEFGTILRPDAAFRVALSY
                     PDLYEIGMSNTAVKLLYAVLNDIPDVSCERVFAVAPDMEAALRSAGEALFSLETGRPL
                     HQFHVVGFSLGYELTLTNLLAILDLGRVPLERKDRGAQDPIVLIGGPAATNPAPLGVF
                     ADCVFIGEAEGWAELAFGELARMKRGGADRGELLSWLQAQPSIWYPGRQDPVRRALWR
                     GFTSRAASTAMPVPNVRVVQDHGVVEIMRGCPNACRFCHATIYYRPCRLKPVGMIHAE
                     VQHLVRMAGYRQVTLSSLSSGDYRGIHDLVRELNALYAPQKVSFSLPSLRVDSLGLSL
                     LAQLSEVRKSGLTFAVETANPDWQREVRKPASLEKTIAILREAKGQGWRSAKFYFMVG
                     LPPAFEEDEPTAIIEFLRAVRAATGMSLNVNVAGFIPKPHTPWERSAQIGEEAAFQRI
                     MAVRDGLKGDGFKIGYHAPFLSLMEGIVSRGDERAGRLVLDAYHRGARLDAWEEYVKT
                     DLWREAIAAADWDVMGTTCRAREPGEALPWDGIALGISSSAITEDYRPVDRAGAVGRS
                     ASGQMPASASSGAWSRVAFLFEKQGAPVFISHLDLMTVFERAILRAGYAARFTEGFNP
                     KPRLEFASPLGLGIESLEEVAAVDLHDFDSAPSFVQRMNAALPAGLEVRTAALVPEPP
                     PSKNPVRRRSLMSLYWGSDYQVEGPEGGSRVLRIASTQPSIRASLTAEGVWETARIKR
                     LRTWARGPESEPRAYFEVLGVAGDPPVSSGASPLAARG"
     gene            complement(4545..>4940)
                     /locus_tag="VFH83_07825"
     CDS             complement(4545..>4940)
                     /locus_tag="VFH83_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET6486312.1"
                     /translation="GSGGFFGKGFLQGTQSHYRFLPQQSTDFLFSILAEEWGFLGALL
                     VFGLFLLILLRGISIVWASREDYATLVGAGILAMVFFHVVVNAGMAMGVMPVTGITLM
                     FLSYGGSSLWTAMMGIGILMNIGRRRMRY"
BASE COUNT          904 a         1674 c         1614 g          748 t
ORIGIN      
        1 cagatcgagt ccctctacag ccagggcctg gtggagtacg ccaaccacaa ctaccagaag
       61 gccatcgatc tctgggagca ggtcctggtc ctggatccgc attatcagcc ggccgcggaa
      121 tacatcgcca cggcgaagaa gaccctcatg ttgcaggagg atctcaccaa ccgcaacccg
      181 cagtagcgac gacgtggaag caaacgaaac ccccgatacc ctttcccatc ccattccgga
      241 gcagaccctg cgacggatcc cccagtatca ccagatcctg gcggagatgc agggccgagg
      301 agaggcctac gtctcctcgc gtcacctcgc ccagttcttc cgaatcgacg acacccaggt
      361 ccgcaaggac gtgtccctga tcggctacaa gggaaagccc aaggcgggat actccatcct
      421 gggcctcaag cgcgccatcg aggagttcct ggggatcaac tacgagaaca cggccatcct
      481 gatcggcgcc ggcaggctgg gctctgccct gagccagtac cccggtctcg ccgagtacgg
      541 tctgaagctc gtggcgatct tcgacaacga ctccgccaag accggcagcg tcctgggagc
      601 cttcaccatc ctgccgatgg aatccctcca gcgggtggtg aggagcttcg acgtagggat
      661 cgcgatcgtg tgcgtcccca agggtgccgc ccaggccgtg gcggggcggg tggtctccct
      721 cgggatcaag gccatctgga acttctcccc tacgcagctt gaagtgccct cggacgtcgt
      781 ggtccgcaac gagaacatcg ccctgggcct ggccatcctt tcccactacg taaagcgcca
      841 gaagcgggac ggacagagcc cgccggcgcc acgggagaac cctcctcagc gtctcatcga
      901 catcttttcc cgataccagc ccaagcggga gtacctggtg cggatcctgg aggagaccca
      961 gaagggatac gggtatctgc cgcgcgacgc agtcgggcac atcgcggact tcctgggcct
     1021 gacccgggag aaggtgcagg aaaccgccgc cttctacccc gttttccgca tggagcctcc
     1081 ggcccggttc cgcatcgtgg tctgcaacgg ggagaactgc gtgcgcaagg gctcccggga
     1141 cctcgtggag tctgtcgccc gagagctcaa gatcggcgtg ggagagacca gcgcggacgg
     1201 cgcattttcc ctggagacgg tgccatgccg cgggctgtgc tccacggcgc cgaacgtgac
     1261 catcaacgac aagctcgtta ccgtccacac cccggaggag cttctggagc acctccgtca
     1321 gctcaatccg ggtcgctgag gtccgccgac gccggagttg cgagccggcg gcttgacacg
     1381 cccagcctcc gccggttcat tgcccgtcat ggaaacactc ccgcaggatg cgttcctgcg
     1441 tttgctcggc atcgtccagc gcctgagggc tcccaacgga tgcccgtggg acctcgagca
     1501 gaccccctcc acgttgaggg cgagcgtcgt cgaggaggcc tgggagtgcg tgagcgcgat
     1561 cgattccggc gacgaggaga accttgccga ggagctgggc gacctctacc tcatggcaac
     1621 catggtctcc tggatgaaag agcaggaggg gacgttctcc gtctcggccg tcctgcaggg
     1681 catctccgac aagctggtcc ggcggcatcc ccacgtgttc ggagaggcgg tggcctcgac
     1741 cccagaggcg gtgctggagc agtgggccgc catcaaggcc acggagaagc cgcggccgac
     1801 ctcccagact tccgccctgg acggagttcc cggatcgctg ccgcccctgg agagggcctt
     1861 cgaggtgcag aagaaggcgg ctaaagtcgg tttcgactgg ccgggtccga agccggtgtg
     1921 ggagaagctc gaagaggaga ccgccgagct tcgggaggcg cgggagtccg ggtccgacgc
     1981 ggccgtggag tcggagcttg gagatctcct cttcaccgtg gtcaacctgt cacggctcct
     2041 gggcgtggat ccctcggtcg cgctcaaccg ggccaacgac aagttcagcc gacgcttccg
     2101 ccaggtggaa gaccggctcc gcgcccaagg gtcggccccc aaggaggcag gcctggagcg
     2161 catggacgcg atctggaacc aggtcaaggc cgaggaggcc ggaagtcagc ctctggccgc
     2221 cagcggcgac gcaccagagg acaccggcgg atcgcctgca acaccgagca cctcgaagta
     2281 ggctcgcggc tcgctttcgg ggccccgcgc ccacgtgcgc agcctcttga tgcgcgcggt
     2341 ctcccaaacc ccctccgcgg tcagggacgc gcggatggaa ggctgcgtgg aggcgattcg
     2401 aagcacgcgg ctgccgccct ccggcccttc cacctggtag tccgagcccc agtacaggga
     2461 catcagagac cttcgccgca cggggttctt gctgggcggc ggctcgggga cgagagccgc
     2521 cgtgcggacc tccaatccgg cgggaagggc cgcgttcatg cgctgaacga agctcggggc
     2581 ggagtcgaag tcgtggagat ccacggcagc cacctcttcc agcgactcga ttccgagacc
     2641 cagagggctg gcgaactcca gacgaggctt cgggttgaac ccctcggtga accgggccgc
     2701 gtagccagcc cggaggatgg cgcgctcgaa caccgtcatg agatccaggt gcgatatgaa
     2761 aaccggagcg ccctgctttt cgaagaggaa cgccacccgg gaccacgccc ccgaggaagc
     2821 ggacgccggc atctggccag acgctgacct cccgaccgcc cctgcccggt ccaccggccg
     2881 gtagtcttcc gtaatggcgc tggacgagat gccgagggcg atcccgtccc agggcagtgc
     2941 ctcacccggc tcccgagccc ggcaggtcgt ccccatcacg tcccagtcgg cggcggcaat
     3001 cgcctctctc cacagatccg tcttgacgta ttcctcccag gcatccaggc gggctcccct
     3061 gtggtaggcg tccaggacca gtctccccgc gcgctcgtcc cctcgggaga cgatcccctc
     3121 catcagggac aggaagggcg cgtggtagcc gatcttgaac ccgtccccct ttaggccgtc
     3181 tctcaccgcc atgatgcgct ggaaggccgc ctcctcgccg atctgggcgg accgctccca
     3241 gggcgtgtgc ggtttcggga tgaaaccagc cacgttcacg ttcagggaca tcccggtggc
     3301 cgcgcgcacc gcgcgcagga actcgatgat ggccgtgggc tcgtcctcct cgaaggccgg
     3361 cggcaggccc accatgaagt agaacttggc ggagcgccac ccttgtccct tcgcctcccg
     3421 caggatggca atggtcttct cgaggctcgc cggcttgcgc acctcccgct gccagtccgg
     3481 gttggcggtc tccacggcaa aggtgaggcc gctcttgcgc acctcagaga gctgagccag
     3541 aaggctcagt cccagcgaat ccacgcgaag ggacggcagg gagaaggaga ccttctgggg
     3601 cgcgtacagg gcgttcagct cgcggaccag gtcgtggatc ccgcggtagt cccccgagga
     3661 gagagaagac aacgtgacct gacggtagcc tgccatccgc accaggtgct gcacttcggc
     3721 gtggatcatg ccgaccggtt tcaatcgaca aggtcgatag tagatcgttg catggcagaa
     3781 ccgacaggcg ttcgggcagc cgcgcatgat ctccacgaca ccgtgatcct ggactacccg
     3841 cacgttcggc acgggcatag cggtgctggc ggctcgagac gtgaagcccc gccacagggc
     3901 gcggcggaca gggtcttgtc ggcccgggta ccagatgctc ggctgagcct ggagccagga
     3961 caggagctcc ccgcggtccg ccccaccccg tttcatccgc gccagctcgc cgaaggcgag
     4021 ctccgcccag ccttccgcct ccccgatgaa cacgcagtcc gcgaaaacgc ccaggggcgc
     4081 cggatttgtg gccgccggtc cgccgatcag cacgatcggg tcctgtgctc cgcgatcctt
     4141 ccgctccagg ggcacgcgcc ccaggtccag aatggcgagc aggttggtaa gggtcagctc
     4201 gtatcccagc gaaaacccga cgacgtggaa ctgatggaga ggtctgcccg tttccaggga
     4261 aaagagcgcc tctccggcgg agcgcagcgc ggcctccata tccggcgcca cggcgaagac
     4321 cctttcgcag gacacgtcgg ggatgtcgtt gaggacggcg tagagcagct tgacggccgt
     4381 gttggacatc ccgatctcgt acagatcggg gtaggagagc gcgacgcgga acgcggcgtc
     4441 cgggcggagg atcgtcccga actccccgcc cacgtaacgg ccgggccggt ccacccggcg
     4501 caggagggag gtcagtcggg cgggcagacg ggtcgtcgcc acgctcagta ccgcatccgc
     4561 cttcgaccga tgttcatgag gatcccgatc cccatcatcg cggtccagag cgacgagccg
     4621 ccgtaggaga ggaacatgag ggtgatgccg gttaccggca tgacccccat cgccatcccc
     4681 gcgttcacca cgacgtgaaa gaagaccatg gccaggatgc cggcgccaac cagcgtggcg
     4741 tagtcctccc gagaggccca gacgatgctg atcccgcgca ggaggatcag gagaaagagc
     4801 ccgaacacca ggagcgcgcc caggaagccc cactcttccg ccaggatcga gaagaggaag
     4861 tccgtgctct gctgaggaag gaagcgatag tggctctggg tgccctggag gaagcccttg
     4921 ccgaagaacc cgccggaccc
//
LOCUS       DASRZS010000318         4740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5565092, whole genome shotgun sequence.
ACCESSION   DASRZS010000318 DASRZS010000000
VERSION     DASRZS010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5565092"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFH83_07830"
     CDS             complement(<1..234)
                     /locus_tag="VFH83_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486313.1"
                     /translation="MNDARDKVVTRRRLTVVAVVLSLLALAVVVQLVRLMVVAPLNES
                     QQTLVIPEVQRGSILDRQGRILAVSVRQQRVSAW"
     gene            311..1573
                     /locus_tag="VFH83_07835"
     CDS             311..1573
                     /locus_tag="VFH83_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET6486314.1"
                     /translation="MITVLERQHIRKRPSSALSKVTGSRDADSPVGPGAYRIRARSIG
                     VQWRSRAPKAAPASPPRLGGLVKRQHPTAPSFLTGQRVAGKRRGPRRLESAARSPRRL
                     AAVLKSPWLLITLAVLLLLAVLLPAALSGRLVTAIRQSIPTVGSRPAVPQPDSTDPDL
                     YRLLIPEDVPPQTPVEPVVKTLALTKYTIKPGDSVGAIASSFKVAVDTVLSWNEIQDA
                     RRLTVGTTLEVPNLDGLKYTVRRGDTLETIARRAGVELNAILDWNQLASPVISVGQEL
                     FLPGAHMDPTDLGRILGTLFQYPVLGRISSYFGNRPDPFTGVVRFHNGLDIVNRPGTP
                     IRAAMAGTVADAAFNANYGNYVILRHPGNYQTLYGHLTRSVVVRNQKVSQGQVIGELG
                     TTGYSTGPHLHFSIFRNLEPVDPLRYLK"
     gene            1586..2605
                     /locus_tag="VFH83_07840"
     CDS             1586..2605
                     /locus_tag="VFH83_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET6486315.1"
                     /translation="MTSTERVRSILRACLVTGYYPRRRAVLSALALTLVTSVVLLTLD
                     ARLPFPRPSEQILYDPSDWFALFRTGTSGQWRPDPARPARPGLGGFSTVQPPVTTLYY
                     RVKPGDTITGIAQRLGLNPDTISSANRPEGRGVHNVSVGELLRIPSQDGVPVTLAGDF
                     DALCAKWKIAPDDVLAANAVSRVDLKQGMALFFPGAQHTGYEFSLSLGVAVRLPLRGA
                     WESSTFGYRADPFTGLRSKHQGVDLAVAYGSPIASATDGVVRNAGWDDILGNYVEVRG
                     QMGYTYIYGHMSRILVKPGMVISQGAILGQVGDTGYATGPHLHFEVRRYGIPQNPALF
                     VPGIR"
     gene            2624..3745
                     /locus_tag="VFH83_07845"
     CDS             2624..3745
                     /locus_tag="VFH83_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486316.1"
                     /translation="MRKLVLAILALLAVFSAIRAYTQAAGTTASNAVPNRPAVGLSLH
                     PGLVEVGDGTSSLGLGFGLMERGGPQRELWSLLPIRMVYTSGDRSVSIGLNGLSFLLR
                     SGVALGRPVLVDRAHTGDVISVGGNVTVDSSVDGDVWTLGADVRLTPRAQVSGDVVAL
                     GGHVVSDPKSVVRGSVSQLPQLKVPFLGVLGSDYSAQALLLARHLLGYVLLGCVLFAA
                     SFYLAAHQQVLVTSVVSVWRPAVITVAVSLVALPVLAALLIASGIGVFLLPLLALGIL
                     LLALDGFLAICSRFGWWLRRGPAPEPAQQRFTSGLLGLFLIKLPSLVGIPLTLIRSAA
                     AARIGDILQQLGLALVAAGLLYGFGAALAQARVRSAAAG"
     gene            complement(3767..>4740)
                     /locus_tag="VFH83_07850"
     CDS             complement(3767..>4740)
                     /locus_tag="VFH83_07850"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HET6486317.1"
                     /translation="DLMRLFGGENIKGLMSRMGMKDGEPIVHPWLNKSIERAQKKVEE
                     RNFEVRKHLLEYDDVLNEQRKFIYSRRDEIVSDPDLKQRVISTGDDIFTEIYDRHKDA
                     GSNAQARVALILAELKESLSFESSVGPAELTEMSTDAMARSIREQLRAGIEAKAAEIG
                     EEVFNRYIQFEYLRNIDSRWQDHLENLEALREAVYLRAYSQKNPLLEYKLEGFQIFDD
                     MLYEIKTAIAQKVFRVRIQQGPTQPAPTAKPAAMQASHGTMGQFAAGASSGTTAISAS
                     ARPMGGNGGAPASTPQAAQVKRTVPKVGRNDPCPCGSGKKYKYCHGR"
BASE COUNT          696 a         1646 c         1533 g          865 t
ORIGIN      
        1 ccacgcggag acgcgctgct ggcggacgct gacggccagg atccgtccct gacggtccag
       61 gatgcttccc cgctggacct ccggaatcac cagggtctgc tgggattcat tcagcggggc
      121 caccaccatg agacggacga gctgtacgac tacggcaagg gcgagaaggg acagaacaac
      181 ggccaccacc gtgagccgcc tgcgggtgac gaccttgtcc ctggcgtcat tcacgaaccc
      241 agtactcccc tccctatcat cggatagtaa tatggcctcg cctaaatgtc gatatggata
      301 acgagcgatt atgatcaccg ttctcgagcg gcagcacatc cgcaagcgtc cctcatcggc
      361 tctgagcaag gtcaccgggt ctcgagacgc cgattctccc gtcggacccg gcgcgtatcg
      421 catccgggcg cgctccatcg gcgtgcagtg gcgctcccgc gcccccaagg ccgcgcccgc
      481 ttcgccgccg cgcctggggg gactcgtcaa gaggcagcat cccaccgcgc cgagcttcct
      541 cacggggcag cgggtggccg gcaagcggcg gggaccccga cgtctggaat cggcggcccg
      601 atctccccgc cgactggccg ccgtgctgaa gagcccctgg ctcctcatca ccctggcggt
      661 tctcctcctg ctggccgtgc tccttcccgc cgcgctgtcc gggcgactgg tgaccgcgat
      721 ccgtcagagc atccccacgg ttggctcgcg acccgccgtg ccgcagccgg attccacgga
      781 cccggatctc taccggctcc tgattccgga ggacgttcct ccgcagacgc cggtcgagcc
      841 cgtggtcaag acgctcgcgc tcacgaaata cacgatcaag ccgggcgatt ccgtaggggc
      901 catcgcctcc agcttcaagg tcgccgtgga cacggtcctg agctggaacg agatccagga
      961 cgcgcggcgt ctcaccgtcg gaacgaccct ggaggtcccc aacctggacg gcctgaagta
     1021 caccgtccgc cgcggagaca ccctggagac gattgcccga cgagcgggcg tggagctgaa
     1081 cgccatcctg gactggaacc agctcgcgag cccggtgatc tcggtcggcc aggagctgtt
     1141 cctgcccggc gcccacatgg atcccacgga cctggggcgg atcctcggga ccctgttcca
     1201 gtaccccgtc ctggggagga tcagctcgta cttcgggaac cgcccggatc cgttcacggg
     1261 tgtggtgcgg ttccacaacg gcctggacat cgtgaaccgt ccgggcactc cgatccgcgc
     1321 cgccatggcc gggacggtgg cggacgccgc gttcaacgcc aactacggca actatgtcat
     1381 cctccgccat ccgggcaatt accagaccct gtacggccac ctgacccgct cggtggtcgt
     1441 ccgaaaccag aaggtcagcc agggccaggt catcggagag ctcggcacca ccggctacag
     1501 tacgggtcca cacctgcact tctcgatctt ccgaaacctc gaaccggttg acccgctgcg
     1561 ctatctcaaa tagagtgggg caggaatgac ctcaaccgag cgcgtccgtt ccatcctgag
     1621 agcgtgcctg gtcactggtt actaccctcg acgccgcgcc gtgctgagcg cgcttgccct
     1681 caccctggtt acgagcgtcg tcctgctcac gctggacgcg cgtctgccct tcccgcggcc
     1741 gtccgagcag atcctgtacg atccttcgga ctggttcgcc ctatttcgga ccgggacctc
     1801 cggccagtgg cgcccggatc ccgcccggcc ggcgcggccg ggcctggggg ggttctcgac
     1861 ggttcaaccc cccgtcacca cgctctatta ccgagtcaag cccggtgaca ccatcacggg
     1921 gatcgcgcag cggctggggc tgaatccgga caccatctcc tccgccaacc gcccggaggg
     1981 acgcggagtg cacaacgtga gcgtcggcga gctcctgcgc attccgtccc aggacggtgt
     2041 accggtgacc ctggcgggag acttcgatgc gctgtgcgca aagtggaaga tcgctccgga
     2101 cgacgtgttg gccgccaacg cggtgagccg cgtggatctc aagcagggca tggcgctctt
     2161 cttcccgggg gcgcagcaca ccggctacga gttcagcctg tccctgggcg tggcggtgag
     2221 gctcccgctg cgcggcgcct gggagtcgtc caccttcggg taccgggccg accccttcac
     2281 gggcctgcgg tccaagcacc agggggtgga cctcgccgtg gcttacggct ctcccatcgc
     2341 ctccgcaacg gatggcgtgg ttcgcaacgc gggatgggac gacatcctgg gtaattacgt
     2401 cgaagttcgt ggccagatgg gctacactta tatctacggc cacatgagca ggatcctggt
     2461 gaagccgggg atggtgatct ctcagggggc gatcctcggc caggtcgggg acaccgggta
     2521 cgccaccgga cctcacctgc acttcgaagt cagaaggtac ggcatccctc agaatcctgc
     2581 cctgtttgtg cctggcatcc gttaactctc gaggggtcgg gacatgagaa agctcgttct
     2641 cgccatcctg gcgctgctcg cggtgttctc cgcgatccgt gcctacaccc aggcagccgg
     2701 gaccaccgcc tccaacgcgg tccccaatcg tccggcggtc gggctcagcc tgcatcccgg
     2761 cctggtcgaa gtgggggacg gcacgagcag cttggggctg ggcttcggcc tcatggagcg
     2821 tggcggccct cagcgcgagc tgtggagcct cctccccatc cgaatggtct acaccagcgg
     2881 agatcggagc gtttccatcg gcctgaacgg cctttccttc ctcctccgct cgggggtggc
     2941 gcttgggagg ccggtcctcg tcgaccgggc ccatacgggg gatgtcatca gcgtgggcgg
     3001 caacgtcacg gtggactcca gcgtggacgg agacgtgtgg accctggggg cggacgtgcg
     3061 gctcacgccg cgcgcgcagg tcagcggcga cgtggtggcg ctggggggac acgtggtctc
     3121 cgacccgaag tccgtcgtcc gcggatccgt gagccagctc ccccagctca aggtcccgtt
     3181 cctgggcgtt ctcggatcgg actactcggc gcaggcgctt ctcctggcgc gccaccttct
     3241 ggggtacgtc ctgctggggt gcgtgctctt cgcggcatcg ttctatctcg ccgcccacca
     3301 acaggtgctc gtgacgtccg tggtttccgt ctggcgcccg gccgtgatca ccgtcgcggt
     3361 ctcgctggtc gcgctccccg tcctggccgc cctcctgatc gcctccggga tcggagtctt
     3421 cctccttccc ctgctggcgc tcgggatcct gctcctggcc ctggacggct tcctggcgat
     3481 ctgctcgcgc ttcggctggt ggctgaggcg gggacccgcg ccggagcccg cgcagcagcg
     3541 gttcacctcc ggtctgctgg ggctcttcct gatcaagctt ccgtccctgg tggggattcc
     3601 gctcacgctg atccgatccg cggcagcggc gcgaatcggt gacatccttc aacagctcgg
     3661 gctggcgctg gtagccgccg gtctgctcta tggtttcggc gctgccctgg cccaggcgcg
     3721 ggtccggtca gcggccgcgg gctgagaggc ccggcgtcgt caggggctac cgcccgtgac
     3781 agtacttgta cttcttcccc gagccgcagg ggcagggatc gtttctcccc accttgggca
     3841 ccgtccgctt gacctgagcc gcctgcgggg ttgaggctgg ggccccgccg tttccaccca
     3901 tcggccgggc cgaggcgctt atcgcggtgg tgccggagga agcgcccgcg gcgaactggc
     3961 ccatggtgcc gtgggacgcc tgcatggcag ccggcttggc cgtgggggcg ggttgcgtcg
     4021 gaccctgctg gatgcgcacg cggaacacct tctgagcgat ggcggtcttg atctcgtaga
     4081 gcatgtcgtc gaagatctgg aacccttcca gcttgtattc caggagcggg ttcttctggc
     4141 tgtacgcccg aaggtacacc gcttcgcgga gcgcctcgag gttctccagg tgatcctgcc
     4201 atcgggagtc gatgttgcgc aggtactcga actggatgta ccggttgaac acctcctccc
     4261 cgatctcggc ggccttggcc tcgatgccgg cgcgaagctg ctcccggatg gacctggcca
     4321 tcgcgtccgt ggacatctcg gtcagctcgg ccgggcccac gctcgactcg aaggaaaggc
     4381 tctctttcag ctccgcgagg atgagcgcca cccgggcctg cgcgttggag ccggcgtcct
     4441 tgtggcgatc gtagatctcc gtgaagatgt cgtccccggt cgagatgacc cgctgcttca
     4501 gatccgggtc gctcacgatc tcgtcccgcc gggagtagat gaacttgcgc tgctcgttga
     4561 gaacgtcgtc gtactccagg aggtgcttgc ggacctcgaa gttccgctcc tccaccttct
     4621 tctgagcgcg ctcaatggac ttgttcagcc acggatggac gatcggctcg ccgtccttca
     4681 tccccatgcg ggacatgagc cccttgatgt tctccccgcc gaagaggcgc atcaggtcgt
//
LOCUS       DASRZS010000319         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5565698, whole genome shotgun sequence.
ACCESSION   DASRZS010000319 DASRZS010000000
VERSION     DASRZS010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5565698"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFH83_07855"
     CDS             complement(<1..317)
                     /locus_tag="VFH83_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486318.1"
                     /translation="MTQVRRALPWVASIAAHGLLLLIPIRIVWQPMIERRVEIDLALQ
                     AVSAPRGVAGPRVGPGKPTINATKSPPRTTTNTRPAAAAGSPQAVQPAAVPPSALRAP
                     APSG"
     gene            658..1290
                     /gene="tmk"
                     /locus_tag="VFH83_07860"
     CDS             658..1290
                     /gene="tmk"
                     /locus_tag="VFH83_07860"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET6486319.1"
                     /translation="MAPIILQRFAVLEGLDGSGTTTQLRLLSERLAREGIPHAATFEP
                     TEGPIGVLLRSILARKASAHPRTVALLFAADRSEHMFDPDDGIEVRTRRGELVVSDRY
                     LYSSLAYQSIECGLEWVSGLNERFPLPQTTIFLDTPVEVCQERLARRSLTELYDGTSF
                     QARVRQTYLEVFERYRGSGARIEVIDGNRPAGLIHEDVWKIVGPMPIKGT"
     gene            1287..1892
                     /locus_tag="VFH83_07865"
     CDS             1287..1892
                     /locus_tag="VFH83_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486320.1"
                     /translation="MKRLALALLLAILPAACAGAAIIYAEQYYQLYHEHLHHYPDDTL
                     EDIFYLEQALKSDIAPFANPLWALATVKDQTDWARYRYLFYMHVNLKLIYCYLTLGSK
                     YEKQNAYFYNAPWKQQNLDSLNLAERVYRVAYGYWDQARQWSTKAWDLRDVHLEQIQE
                     WEDESYRIETGDLDYRDIIDSALAHLAKIRAAFQAMDQSTY"
     gene            complement(1897..>2320)
                     /locus_tag="VFH83_07870"
     CDS             complement(1897..>2320)
                     /locus_tag="VFH83_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HET6486321.1"
                     /translation="WVTLHVGLGTFQPIRSEEIEDHPMHEERFHVPQETRRLVNQAVE
                     GEQPVLAVGTTVVRTLESAWSSDGLRAGDGATRLFITPGYRFQVVSQLFTNFHTPGSS
                     LLVLVSAFAGTERILSAYAQAVRKRYRFFSYGDATLIR"
BASE COUNT          416 a          735 c          773 g          396 t
ORIGIN      
        1 cctgagggag ccggagcccg gagggccgat ggggggacgg cggccggttg gaccgcctgc
       61 gggctccccg ccgcggcggc cggccttgtg ttcgtcgtgg tccgcggagg gcttttcgtt
      121 gcgttgatgg tgggcttccc aggacctacc ctgggtcccg ccacgccacg gggcgcgctc
      181 acggcctgga gcgcgagatc gatctcgacc cttcgttcga tcatcggctg ccacacgatt
      241 cggatcggaa tcaggaggag cagcccgtgg gcggctatcg aggctaccca cggcagcgct
      301 cgtcgaacct gggtcatggg ccggagcttc ccttggccag gggcctctgc tccacggacc
      361 cggggatcca ttccgtaaac gccatccagg gcgcggacca ggctcctgcg ggtggcggca
      421 cgggcggtag caccggcgaa ggcggcggtg taaggtccag ggaggggatg atcacggggg
      481 gaagaatcca cggcggcggg agcggaagca cggtccggtc ggtgcccacg acggtcaccg
      541 cctgatccac ggggagaggt tcgctcgctt gaggtgtctg cgcggaagcc atgacgctcg
      601 cggcgaggag cagcgctgga atgacatggg ccgctgttct cggtacaatc atcgccggtg
      661 gctcccataa tactgcaaag gttcgccgta ctggaaggcc tggatggttc gggaacgaca
      721 acgcagcttc ggcttctgtc ggagcggctg gcccgggagg ggataccgca cgccgccacc
      781 ttcgaaccga ccgagggacc gatcggggtc ttgcttcgat ctatccttgc ccgcaaggcc
      841 tccgcgcatc cccgaacggt tgcgctgctc ttcgccgcgg atcggagcga gcacatgttc
      901 gaccccgacg acggaatcga ggtgaggacc cgccgggggg agctggtggt ctccgatcgg
      961 tacctctatt ccagcctcgc ctaccagtcc atcgaatgcg gactggagtg ggtctccggc
     1021 ctcaacgagc ggtttcctct cccgcagacc acgatcttcc tggacacccc cgtggaagta
     1081 tgccaggagc ggctggcacg ccgttctctc acggagctct atgatggcac gagcttccag
     1141 gccagggtgc gccagaccta cctcgaggtg ttcgagcggt accggggatc cggagcgcgg
     1201 atcgaggtca tcgacgggaa tcgtccggcc ggcctcatcc acgaggacgt ctggaaaatc
     1261 gtcggtccca tgccgataaa gggaacgtga agcggctcgc cttagccttg ctcctggcca
     1321 tactaccggc agcttgtgcc ggcgccgcca ttatctacgc ggagcagtac taccagctct
     1381 atcacgagca cctccatcac tacccggatg acacgctgga ggacatcttc taccttgagc
     1441 aggctctgaa gtcggatatc gcgccgttcg ccaacccgtt gtgggccctc gctacggtca
     1501 aggatcagac cgactgggcg cgctaccggt atctcttcta catgcacgtg aacctgaagc
     1561 tgatctactg ctacctcacg ctggggagca agtatgagaa gcagaacgcc tacttctaca
     1621 acgctccctg gaagcagcag aacctggaca gcctcaacct cgccgagaga gtgtatcggg
     1681 ttgcctacgg atactgggac caggcgcgac agtggtccac caaggcgtgg gacctgaggg
     1741 acgtgcacct cgagcagatc caggagtggg aggacgagag ctaccgcatc gagaccgggg
     1801 acctggacta ccgggacatc atcgactccg cccttgccca tctggcgaag atccgagccg
     1861 ccttccaggc gatggaccag agcacgtact gacgcgtcag cggatcagcg tggcgtctcc
     1921 gtagctgaag aagcgatacc gcttccgcac ggcttgcgcg tatgcggaga ggatccgctc
     1981 ggtaccggcg aaggcgctga ccagtacgag caggctggag ccgggcgtgt ggaaattggt
     2041 gaacagctgg ctcacgacct gaaaccggta gcccggagtg atgaagagcc gggtagctcc
     2101 gtctcccgct cgcaacccat cggaggacca ggcagactcc agggtccgca ccaccgtcgt
     2161 gccgaccgcc agaacgggct gctcgccttc caccgcctgg ttgaccagtc ggcgcgtttc
     2221 ctgcggcacg tggaaccgtt cctcgtgcat cgggtggtcc tcgatctctt ccgatcggat
     2281 gggctggaag gtcccgagcc cgacgtggag ggtgacccag
//
LOCUS       DASRZS010000320         2942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4025145, whole genome shotgun sequence.
ACCESSION   DASRZS010000320 DASRZS010000000
VERSION     DASRZS010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4025145"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2420
                     /locus_tag="VFH83_07875"
     CDS             <1..2420
                     /locus_tag="VFH83_07875"
                     /inference="COORDINATES: protein motif:HMM:NF019368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HET6486322.1"
                     /translation="IDLAWKKDRQEMAELFEAFVVRLLDLLDRNPSLTYVLEQAAHFR
                     QLAAARPDLISRVRTYVRAGRLEVVGGMASTMETNTPNGECFLRNQLLGLGWFRENLG
                     TRPAAGWLIDTFGIHAQVPQVLCQLGIPYLFANRLGGTVDVTMVRARGLDGSEVPVIG
                     RDVYSPYVKPGSLAFRHVQSWEQIQALFQDAARLSGTGPFLVVPYTENETLPSSALIH
                     AAERMSSLDPAATWSFAVPSQLNAQVRDASAQWPQLDGDLNPEFTGTYAHRSIIRRRN
                     RAVENKLLDAEKWLSLLSAPGWGQDFEECWWKMAFVHFHDVFTGSHPTKVRDQVLTTL
                     DEVESTVAAHRDPLLSEHCRVDPSAGGRTLFVANSLPWERRDVQRLELPDGIGVVQRV
                     ASPEGDVPFWQNGRQLWMRTALPSMGIAAYRLELGDAPAAASADAASITSPSGSIENR
                     LVRASFRAESGLAGITLRGAGVELCVCTGVELVAQQDDGNFQIEDPVGSEIPASSGVH
                     SLRLYPITPLGQRATVHGAFPELPWSPGSSLQWEAEVLLPVDSDRLELGIRLRWRGEG
                     TRIRLRVQTPVETSSCIQEVPFGTVRRRPYRDRGTARGEWPVQRFVALEDHRGGLALI
                     NRGTVGVEAAGGTLWTTLLRAPVSEYAGMVPDDTSSQHGEHEFHFTLVPYSGSWHEAG
                     IHRAAAEANSPPVVLLRPGRPTGLPIASLLRLDAATVMLSTVKAASDGSGEIVARLYE
                     TAGIRDRAVLEMDGAEAAWDSTLEELRGSPLACEGQRVVLDLRPFEIRTIRIRKRYRE
                     IERREKS"
     gene            2417..>2942
                     /locus_tag="VFH83_07880"
     CDS             2417..>2942
                     /locus_tag="VFH83_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6486323.1"
                     /translation="MRSKRVILLLAAALALLVVVPVGATGKGEAQAPTKAETFSLYVG
                     DFWQTNYIDPSWTDPVAKEITRRTGVTLQVSVVKSEDDDGEMNKLIAAGDLTDLIFRG
                     GGSSKQALAKGGLVKPLDDLIEKYGPNIKKYHAGTFNAWRNRSDGKIYSLGFWYFNQV
                     SKFGLNLQVSTLQMR"
BASE COUNT          516 a          926 c          985 g          515 t
ORIGIN      
        1 acatcgatct tgcctggaag aaggaccgcc aggagatggc ggagctcttc gaggccttcg
       61 ttgtccgtct gctggacctc ctggaccgaa atccctcctt gacctatgtg ctcgagcagg
      121 ccgcccactt ccgccaactg gcggcggcaa gacccgatct gatctcgagg gtaagaacct
      181 acgtacgggc cggccgcctg gaagtggtgg gcggaatggc ttccacgatg gaaacgaaca
      241 cccccaacgg ggagtgtttt ctacgcaacc aactcctggg gctgggctgg ttccgcgaga
      301 acctgggtac tagacccgct gcgggatggc tgatcgacac ctttggcatc catgctcagg
      361 ttccccaggt tctgtgccag cttggaatcc cctacctgtt tgccaaccgg cttggcggca
      421 ccgtggacgt cacgatggtc cgagcgcgcg gcctggacgg ctctgaggta ccggtgatag
      481 gccgagacgt gtactccccg tacgtaaagc cgggatcgct tgcgtttcga cacgtccaga
      541 gctgggagca gatccaggcc ctgtttcagg acgcagcgcg cctttcgggc accggcccgt
      601 tcctggtcgt gccatacacc gagaacgaga ccctgccgag cagcgcgttg atccatgcag
      661 cggaacggat gagctctctg gaccctgccg ccacgtggtc cttcgcggtg ccgtctcagc
      721 tcaacgcgca ggtgcgggac gcttcggccc agtggccgca gctcgatgga gacttgaacc
      781 cggagttcac ggggacctat gcccaccggt ccatcattcg cagacgcaac cgcgcggtgg
      841 agaacaagct tctggacgcg gaaaagtggc tctcccttct ctccgcgccg ggctgggggc
      901 aggacttcga ggaatgctgg tggaagatgg ccttcgtgca ttttcacgac gtgttcacgg
      961 gatcccaccc gacgaaggtg cgcgatcagg tcctcacgac ccttgatgag gtcgaatcga
     1021 cggttgcggc gcatcgggac cctctcctgt ccgaacactg cagagtagac ccctccgcgg
     1081 gcggccgcac gctcttcgtc gcgaacagcc ttccctggga gagacgcgat gtccagcgcc
     1141 tggagcttcc ggacggaatc ggggtagtgc agagggttgc gtcgccggaa ggagacgtcc
     1201 cgttctggca gaacggccgt cagctctgga tgcgaaccgc gcttccctcg atggggatcg
     1261 cggcgtaccg cctggagctg ggagacgctc cggccgcggc gtcggccgac gcggccagca
     1321 tcacgtcccc ttctggctcc atcgaaaaca ggctcgtgcg agcctccttc cgtgccgaat
     1381 ccggattggc ggggatcact ctgagaggcg cgggcgtcga gctctgcgtc tgcaccggcg
     1441 tggaactggt agcgcagcag gacgatggca acttccagat cgaggatcct gtcggctcgg
     1501 agatcccggc ttcttccggc gtccacagtc tcaggctcta cccgataacg ccactagggc
     1561 agcgtgcaac ggttcatggc gcgtttcctg agctgccgtg gtccccgggc agctcgctgc
     1621 agtgggaagc ggaggttttg ctgccggtgg acagcgatcg cttggagctc gggatccggc
     1681 tccgatggag aggcgagggg acccggatcc ggctccgagt ccagaccccg gtggaaacct
     1741 cctcctgcat tcaggaggtc ccgttcggga cggtccgccg aaggccgtac cgggaccggg
     1801 gcacggcccg aggcgagtgg ccggtgcagc gcttcgtagc gctggaggat cacaggggcg
     1861 gactggcgct gatcaaccgg ggcaccgtcg gcgtagaggc ggccggcggc acgctgtgga
     1921 ccacgctgct gcgggcgccg gtctccgagt atgcgggcat ggttccggat gacacctcat
     1981 cccagcatgg cgagcacgag tttcacttca ccctggttcc ttactcagga tcctggcacg
     2041 aggccgggat ccatcgggcg gccgccgagg cgaactctcc cccggttgtc ctgctgcgcc
     2101 ccggccggcc aaccggcctt ccgatcgcgt cgttgctgcg gctcgatgcc gctaccgtga
     2161 tgctttccac ggtgaaggcc gcctcggacg gcagcgggga gatcgtggct cggctctacg
     2221 aaaccgcggg gattcgggac cgcgcggtcc tggagatgga cggcgcggag gctgcatggg
     2281 actctaccct ggaggagctt cggggatccc cgctggcatg cgagggacag cgggtcgtcc
     2341 tcgacctgag accgttcgaa atccggacca tccggatccg aaagaggtat cgcgaaatcg
     2401 agaggaggga gaagtcatga gatcgaaacg cgtcatcctg ctgctggccg cggcgctggc
     2461 cctgctcgtc gtcgttcccg tcggagccac ggggaaaggc gaggcgcaag ctcccacgaa
     2521 ggccgagacg ttcagcctgt acgtggggga cttctggcag accaactaca tcgatccgtc
     2581 gtggacggat ccggtcgcca aggagatcac gcggcgaacg ggcgtcaccc tgcaggtcag
     2641 tgtggtcaag tccgaggacg atgatggaga gatgaacaag ctcatcgctg ccggagacct
     2701 gacggacctc atcttccgcg gagggggctc ttccaagcag gccctggcaa agggcggact
     2761 cgtgaagccc ctggacgacc tgatcgagaa gtacggcccg aacatcaaga agtatcacgc
     2821 cgggacgttc aacgcctggc gcaaccggtc ggacggcaag atctacagcc tgggtttctg
     2881 gtacttcaac caggtgtcga agttcggcct caacctccag gtcagcaccc ttcagatgcg
     2941 gt
//
LOCUS       DASRZS010000321         4957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5178929, whole genome shotgun sequence.
ACCESSION   DASRZS010000321 DASRZS010000000
VERSION     DASRZS010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5178929"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="VFH83_07885"
     CDS             <1..170
                     /locus_tag="VFH83_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET6486324.1"
                     /translation="EIIQYRFGLNGKRPMSLKEIGSLYKLTKERIRQIEKKAIKRLQH
                     PARNQALRAYL"
     gene            complement(244..1170)
                     /locus_tag="VFH83_07890"
     CDS             complement(244..1170)
                     /locus_tag="VFH83_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6486325.1"
                     /translation="MSIRLTFWGVRGSIPVPGPTTVKYGGNTACLELRFGTKDRLIVI
                     DAGSGIRELGGSIVKRDLPKGPVNTKIFLTHTHWDHIMGFPFFTPIFIPGTQLQIYGP
                     VTYEEDTLDRIVGDQLQYRYFPVRVEELRANIEYFPLKEGSLDLGDGLSVTTKYLNHP
                     ILVLGYRFQYEGKTLCTAYDHEMFNNVFDAKPEDPGYDPAAVKEGALAAQEENQKVAA
                     FYAGADLLVHDTQYTHKEYLSGKKGWGHSSFEWAINSAHKAGVKHLVLFHHEPVRCDQ
                     ELEALHQAYVKSIQGKTSMKISLAQEGKTFEV"
     gene            complement(1180..2052)
                     /locus_tag="VFH83_07895"
     CDS             complement(1180..2052)
                     /locus_tag="VFH83_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET6486326.1"
                     /translation="MQDKERILAVIQAYIARMPSLPVTVSKILQICNDPKTSPSDLNQ
                     VISLDPVLMGRVMKLINSAYYSLNNQITSLVRAIIMLGINTVKNLALSTAVLGNLGGK
                     GNFQALNIDGFWRHSLCVGVMAKLIAIRRKRDNKVLEEYFVAGLLHDIGKIPLNNKLS
                     AEYMNAIALTDKDHSPLYLVEERVMEVNHCEVGRLIIKNWNLGAAMEDVVYFHHFISQ
                     YPGENRDLLLTVAAANFFANSYQIGFSGDRYPEKLPLEVFEELGISLDDLESMEQKAN
                     AEIEKAKVFLSVSV"
     gene            complement(2056..>4957)
                     /locus_tag="VFH83_07900"
     CDS             complement(2056..>4957)
                     /locus_tag="VFH83_07900"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6486327.1"
                     /translation="QKGLQVDANQPRLRFNLGVVFERTGKLKEARAEYEEAARALPAW
                     GDAKNNLAVTLHRLGEDAEAEKVLRSALAAEPGNAKVRNNLGVVLAAMGRSEEATQLY
                     RDAIRADPRYAHAVGNLALLMEQGGKLTEAMEELRRLLYLEPKNTDARLRLAGILRAL
                     ENFPDAIKNYQGVLASQPANLDAWKGLALAYNRSGDREKALACYKKLESTSAADQDWR
                     LDRAALLREAGDLAHAREEVNRYLAAVPDDRPGRLLLADIYAKEGHPRQAAQTLRDVL
                     GSFPEDQEAGHRLAQLYRQLGEPTKAIETLETLINRLEQGGDPKDLDALMRTMEEYEQ
                     AIAEHEKDYRQERERAIRKLRELAADTSLKEKERGEADRLIIEEMEPLKEDAVPIINV
                     GGLEPVFAVREEDEELKLEERDEPIHDETVDIEDERPPNLVNLLKDQELYAENPALQM
                     FEPMPLLPSQHPQGFAPGAAAPQAPAGTAPPAMAPAPPAFPAAAPPVAHALAQPAPAP
                     APQPVFQQPALPQPVFAQPPLQPPAPALQHLPSPQILPQPGPAPQPPAQPQAAPAYQP
                     APSAPSAPPPLPAPTAPSGPSAPADAMLAMSLREAAQSQQQMADRLFSELQSLSRKIQ
                     ERAPSGPPATRSTGRRPAPTEAEEPQGFPRFIPSGEGEPGEEEEPRLEEPAVGPAAPR
                     GQRAMGEILEELPVEDVAPEGPLPPPEEELPVAEEPVAQAAAGGAAEPEEDGARAAAE
                     DGRPEADTQDTVPPETRATEEPGESAMGDETPSATEPEAILEEVPEDAFDEEEGAGAE
                     AASPEEGSGGPAPGPPGQKAAPESAAPSTGPRPTDEVRKELRDYISGVRQKLNGAAVQ
                     PSNPGALLDYLAKLSDYLPESERRRFRGSTERLTMESLKAQLAGKQGLRKSIGQKFPA
                     GGHEPKEPLSRSRVVETFSFLSGMANHHPDRAIGAAMVEQIGSLVARLRSSG"
BASE COUNT          745 a         1639 c         1627 g          946 t
ORIGIN      
        1 cggagatcat ccagtaccgc ttcggactga acggcaagcg gccgatgtcc ctcaaggaga
       61 tcggcagtct ctacaagctg accaaggagc gcatccgcca gatcgagaag aaggccatca
      121 agcggctgca gcatcctgcc cgcaaccagg cgcttcgggc ctacctgtag cgcctgggat
      181 cgagccccga tggcggtccg ctccatccgt cggggctctg acgaatcccg ccggagcgcc
      241 cggctacacc tcgaaggtct ttccctcctg agcgagggag atcttcatgc tcgtcttgcc
      301 ctggatcgat ttcacgtagg cctggtgaag ggcctccagc tcctggtcgc aacggacggg
      361 ctcgtggtgg aagagcacca gatgcttcac cccggccttg tgggctgagt tgatggccca
      421 ctcgaacgag ctgtgccccc atcccttttt tcccgagagg tattccttgt gcgtgtactg
      481 cgtgtcgtgg accagcaggt ccgcgcccgc atagaaggcg gccaccttct ggttctcttc
      541 ctgcgccgcc agagcccctt ccttcacggc ggccgggtcg tagccgggat cctcgggctt
      601 cgcgtcgaac acgttgttga acatctcgtg gtcgtaggcc gtacagaggg tcttgccctc
      661 gtactggaac cggtagccga ggaccaggat ggggtggttg aggtacttcg tggtgaccga
      721 gaggccgtcc cccaggtcca gcgatccctc cttgagcggg aagtactcga tgttggcgcg
      781 cagctcctcc acgcgcaccg ggaagtaccg gtactgaagc tggtccccga cgatccggtc
      841 cagcgtgtcc tcctcatagg tgacgggccc gtagatctga agctgggtgc cggggatgaa
      901 gatcggggtg aagaagggaa accccatgat gtgatcccag tgcgtgtggg tcaggaagat
      961 tttggtgttg acgggcccct tgggcagatc ccgtttcacg atggagccgc ccagctcgcg
     1021 gatgccggag cccgcgtcga tgacgatcag ccggtctttg gttccgaagc ggagctccag
     1081 gcaggccgtg ttgccgccgt acttcaccgt cgtcggcccg gggaccggaa tggacccacg
     1141 cactccccag aaggtcaggc ggatggacac ggccagccct tagacggaga cgctcaagaa
     1201 gaccttggcc ttctcgatct ccgcgttcgc cttctgctcc atggactcca ggtcgtccag
     1261 agagatgccc agctcctcga atacctccag cggcagcttc tccggatagc ggtcgcccga
     1321 gaacccgatc tggtagctgt tggcgaagaa gttggccgcg gccaccgtga ggaggaggtc
     1381 gcggttctct ccgggatact ggctgatgaa gtgatggaag tacaccacgt cttccatcgc
     1441 cgcccccagg ttccagttct tgatgatcag gcggcccacc tcgcagtggt tgacctccat
     1501 cacccgctcc tccacgaggt acagcgggga gtggtccttg tccgtcaggg cgatggcgtt
     1561 catgtactcc gccgagagct tgttgttcag cgggatcttc ccgatgtcat gcagcaggcc
     1621 ggccacgaag tactcttcca gcaccttgtt gtcccgcttg cgccggatgg cgatgagctt
     1681 ggccatcacc ccgacgcaga gcgagtggcg ccagaatccg tcgatgttca gagcctggaa
     1741 atttcccttg ccgcccaggt tgcccaggac cgcggtggag agcgcgaggt tcttcaccgt
     1801 gttgatcccc agcatgatga tggcgcggac cagggaggtg atctggttgt tgaggctgta
     1861 gtacgcggag ttgatgagct tcatgacccg tcccatcagt acggggtcca ggctgatgac
     1921 ctggttcagg tcactcggcg aggtcttcgg atcgttgcag atctgcagaa tcttggagac
     1981 ggtgacgggg agactcggca tacgcgcgat gtaggcctgg atcaccgcga ggatccgctc
     2041 tttgtcctgc ataccctacc ctgaactcct caagcgtgct accagggaac caatctgctc
     2101 taccatcgcc gcaccaatcg cccggtccgg atggtgattc gccatcccgc tgaggaagga
     2161 gaacgtttcc accactcgag agcggctcaa cggctccttg ggctcgtgcc cgcccgcagg
     2221 gaacttttgg ccgatcgact ttcgcaggcc ctgctttccc gcaagctggg ccttcaggga
     2281 ctccatggtc agccgctcgg tgcttcccct aaaacgccgg cgctcactct ccgggagata
     2341 atcggaaagt ttggcgagat agtcaaggag agctcccggg tttgagggct gaacggcggc
     2401 tccgttgagc ttctggcgca cgccgctgat gtagtcccgc agctcctttc gaacttcgtc
     2461 ggtcggccga ggccccgtgg agggcgcagc gctctcggga gctgccttct gcccgggggg
     2521 cccgggcgcc ggaccgccgg aaccctcttc cgggctggcg gcctccgctc cagctccttc
     2581 ctcttcgtcg aaggcgtcct ccggcacctc ctcgaggatc gcctccggct cggtggctga
     2641 cggtgtttcg tcacccatcg cgctctcacc gggctcctcg gtggcacgtg tctctggcgg
     2701 gacagtgtcc tgcgtgtccg cttcggggcg cccgtcctct gcggcagccc tggcaccgtc
     2761 ctcttcgggt tccgcggccc ctccagccgc cgcctgagcg acaggttcct cggcgaccgg
     2821 cagctcctct tccggaggcg gcaacggccc ctccggagca acgtcctcca ccggaagctc
     2881 ctcgaggatc tcgcccatgg cgcgttgccc gcggggcgcg gcgggcccga ccgccggctc
     2941 ctccagccgt ggctcttcct cctcgccagg ctccccctcc ccggatggaa tgaaccgagg
     3001 gaacccctgg ggctcctccg cctccgtcgg cgcggggcga cggccggtgg accgggttgc
     3061 gggcgggccc gaaggcgccc gctcctggat cttccgagac aggctctgca gctccgagaa
     3121 cagacggtcc gccatctgct gttggctctg tgccgcctcg cgcagggaca tagccagcat
     3181 cgcatcggcc ggtgcggacg ggccggaagg agccgttggt gccggtaatg gcggtggcgc
     3241 cgacggcgcc gatggcgcgg gctgataggc cggtgccgcc tgaggttgcg ccggcggctg
     3301 cggcgctggc cctggttggg gaaggatctg tggggacggg agatgctgaa gggccggcgc
     3361 tgggggctga aggggcggct gcgcgaaaac gggctggggg agggccggct gctggaacac
     3421 cggctgcggc gcgggagccg gagctggctg cgccagggca tgagcgacag gcggcgcggc
     3481 ggcagggaag gccggcggag ccggagccat ggccgggggg gcggttccag caggcgcctg
     3541 cggggcggcc gctccgggag caaaaccctg cgggtgctgg gagggaagga gaggcatggg
     3601 ctcgaacatc tgaagggccg ggttctccgc gtagagctcc tgatccttca ggaggttcac
     3661 caggttcggc ggcctctcgt cctcgatgtc aaccgtctcg tcgtggatgg gctcatcccg
     3721 ctcctcgagc ttcagctcct cgtcctcttc ccgaaccgcg aagaccggct ccaaccctcc
     3781 cacgttgatg atgggcaccg cgtcttcctt gagcggttcc atctcctcga tgatgagccg
     3841 gtccgcctcg ccgcgctctt tttccttcag ggaggtatcc gccgccagct cccgcagctt
     3901 gcggatggcc cgctcgcgct cctgacggta gtctttctcg tgttcggcga tggcctgctc
     3961 gtattcctcc atggtccgca tcaaggcgtc gaggtccttg gggtctccgc cctgctccag
     4021 gcggttgatg agggtctcca gggtctcgat tgccttcgtc ggctctccta gctgccgata
     4081 gagctgggcc agtcggtggc cggcctcctg gtcctccggg aaggagccca gcacatcccg
     4141 caaggtctgg gcggcctggc gcgggtgccc ttccttggcg tagatgtccg cgagcaggag
     4201 ccggccaggc cggtcgtccg gcaccgcggc gagataccga ttcacttcct cgcgagcatg
     4261 ggccagatcg cccgcctctc ggaggagggc cgcgcggtcc aggcgccagt cctggtccgc
     4321 cgcgctggtg gattcgagct tcttgtaaca ggcaagcgcc ttttcccggt ctcccgagcg
     4381 attgtaggcg agggcgagcc ccttccatgc atcgagattg gcaggctggg atgcaaggac
     4441 cccctggtag ttcttgatgg cgtccggaaa gttctccagg gcacgcagga tcccggccag
     4501 acgcagacgc gcatccgtat tcttgggttc caggtagagc aggcgacgca gctcctccat
     4561 cgcctccgtc agcttgccgc cctgttccat cagcagcgca aggttcccga ccgcatgcgc
     4621 gtaacgggga tcggcacgga tggcgtcccg gtatagctgg gtggcctcct cggatcgacc
     4681 catcgcggcc agaacgacgc cgaggttgtt ccgcaccttg gcgttcccgg gttccgcggc
     4741 cagcgcgctc cgtagaactt tctccgcctc cgcatcctct cccagccgat gcagagtaac
     4801 cgccagattg ttcttcgcgt ccccccacgc agggagcgcc cgagcggcct cctcgtactc
     4861 tgcccgggcc tctttgagct tgcccgtgcg ctcgaagaca acccccaggt tgaaccgcaa
     4921 ccggggctga ttggcgtcca cctgcagacc cttctgg
//
LOCUS       DASRZS010000322         9901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2099425, whole genome shotgun sequence.
ACCESSION   DASRZS010000322 DASRZS010000000
VERSION     DASRZS010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2099425"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1064)
                     /locus_tag="VFH83_07905"
     CDS             complement(84..1064)
                     /locus_tag="VFH83_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET6486328.1"
                     /translation="MSASLDPAVAKGKLTTMEECIRLGCTRAFEGVRADHLAVEPAGV
                     TSLTLGKGNENLLLFAARGVIALRGGPAAPADPLARALRAHGDVVLCPSEAFLLSAFH
                     GELVLQRSPSSELYILSFLQPPPRAARSRVGGSSGDAWSHRLEIPDHVTVLRPARLTH
                     LLRRLMDETRRPSPSRAILHHLVALALCELAHSSRASERTENREAALESIASRVDAYI
                     AAHYHEPIGTPDIAAELRYNPDYLERAYHLERRISIREAVHDRRIKEVRAQLLLQRGK
                     GVAEVAALCGYADPAYFRRVFKRATNMTPCGYRRVHADSLAGSAVGAAAR"
     gene            complement(1101..3062)
                     /locus_tag="VFH83_07910"
     CDS             complement(1101..3062)
                     /locus_tag="VFH83_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HET6486329.1"
                     /translation="MSEGFPVSAQPRSKIDGGFWKGYQDLLRNKVIPYQWKAMNDEIP
                     GAPKSHSVENFRIAAGVRSGTYEGMVFQDSDLAKWLEAAGYLLAEEGAGNSALRAWVD
                     EAVDLVSRAQQPDGYLNTYFTVKEPSARWTNLREAHELYSAGHMIEAGVSLYLGAGNQ
                     RILEVVKRLADHIDSRFGPQAGKVRGYCGHEEVELALVKLARATGETRYLRLASYFVD
                     ERGRSPSYFQTEAAAPGYHPFFGIKSLDYYQAHAPVREQPDAVGHAVRAVYLYTAMAD
                     LAQETGDPSLAEACERLWQSVTRRKMYITGGIGSSAHLESFGADFDLPNDIAYAETCA
                     AIGLFFFSARMVRLRSQAKYADVMERALHNGILSGLALDGESYFYVNPLGVIPAVCDA
                     NGGYRHVKYRRQPWYGCACCPPNIARLLASLGQYAYHCSGDALYADLFHEGTVTVGIG
                     NDTIRLKQSTRYPWDGRIRFQWLSETRSDLTLAVRIPSWCVGAPLQVNGAAVAAGADA
                     DGYARVRRRWSAGDTVELTLPLTIQRMRADPRVAGNRGRVAIQRGPIVYCLEEADNGD
                     NLAGIRLPQDAVLEASHRGDLLGGVTVIRTTGLSDAGERGAAGSTTTDGELYSNRGAP
                     RADRKELTFVPYYAWANRTPGEMVVWVRE"
     gene            3290..4618
                     /locus_tag="VFH83_07915"
     CDS             3290..4618
                     /locus_tag="VFH83_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET6486330.1"
                     /translation="MMRRLGMAAGLAGALLLLMALTAGADPVKIQYGYWGDQNEETST
                     TALLQTFMDAHPNIQVEGLRFGSNTQFNDKITTLAAAGTLPDVSTFYEPNVLTWGMNG
                     QFVDLTDFYKKAAPKLDAIKFVTPDGKIVGISVANEIQVIWYNKRIFDAANLPYPPAD
                     PAKAWTWAQFVSVAKKLTRDSNGKTPNDKGFDPSNIRTFGAWVQQWWMPWLTFAVSNG
                     GGMVSQDGKRLMMGDPATIDAIQKMADLINVDHVSPSPQSQQMPTGVATSLLSGQVAM
                     AMDGTWDLLTVGTTKDQQGLDFGIGVLPYMKKLATTSVGTPEVVYKSTQHLDEALALL
                     GYLMDPASSLPLLQNGIWLPNEKQWYTDPTLLQKWIGNPRHPPEYKQAVVNFALNYSN
                     RLPLYYVPTFGRMDDVIEAALGQVWLGQKTAANVIKNEIMPKITPIFQGQ"
     gene            4719..5624
                     /locus_tag="VFH83_07920"
     CDS             4719..5624
                     /locus_tag="VFH83_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018752004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6486331.1"
                     /translation="MKRRRLRASTTEAIAGYLFASPVILGLVIWVILPMFGVLAISLT
                     DWNVLRAPRWVGVQNYVKLFTTDLYFWNSLKVTAYFVVLNVACTVLYSLVVALLLNQK
                     IRGRGIYRSFFYLPTIIPVVASSALWSWLFNPEFGLLNVLLQAVKLHRSLWLAAQTTV
                     VPSIVLMTVWAGAGNTIVIFLAALQGVPRELLEAVEIDGGTWWHRFRAITLPMISPVT
                     FFNVVTGLIASFTIFTQSYIMTNGGPNNASLFYVLLLYREAFQRNNFGGAAALSWVLI
                     IIVSVLSLLIFRTSSRWVYYEGEAR"
     gene            5621..6460
                     /locus_tag="VFH83_07925"
     CDS             5621..6460
                     /locus_tag="VFH83_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015845162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6486332.1"
                     /translation="MRLSVARVTGRGVVYLLLTIGAAFCILPVFWLVRSSAMTLGDIF
                     KFPPILWPATIRWQNFADAMKASPFLRYFRNTMIVVVPNVAGALLTASMSAYGLARFR
                     FPGRNFWFSLVLGAIILPPAVVLIPQFLLWSKLHGVNTFLPLIVPVWFGGGAFNVFLL
                     RQFFLTVPRDFDDAARMDGATYWRTYWQIMIPFIQPALLAVGLFQFLFAWNDFFNPLI
                     YLSSKATYTLAVGLQFFVGSYGTFWNILMAAAVVIVVPPVIVFLIGQRYFVEGVTLTG
                     IKG"
     gene            complement(6450..8501)
                     /locus_tag="VFH83_07930"
     CDS             complement(6450..8501)
                     /locus_tag="VFH83_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6486333.1"
                     /translation="MFKNLRFGARLIIVGTILLVIPLAVVAYLAVQRSSLAISAIETE
                     QLGRSARLVAASINDVFVQEKKFAMGQALQDQIVAAAVAPRGMALGGDAIARATDRLL
                     AVKNQKGLGEGFETILCVGRDGVAFAATDPSYVGVSFADRNYIKQALAGNANAGAAVI
                     SKVTGKPVVPVAAPIRSGNEVVGVYVSILEAGFLNDLIINERIGRSGYAYVVDHDGLI
                     IAHPVADNVFKTNLASLDGTRDFTRKMIAGESGVSTYVFNGVAKTAGYAPVTETGWSV
                     GYTLPDVEYLAPSRDLRNLILVIGAAALAAAFLVYLVFARSVSRPLEKGVAFAEVVAS
                     GDLTQRLAIQRNDEIGKLAAALNSMSGKLRAMVSTIQENAVQVASSSEEISASAQKLA
                     EGAQNEASTLEETSASVEELSASVDQVAEHAQTQAAAAEQGSASMAQVHQSIEDVSRH
                     LTEIASLSGRSLENALEGAKAVSEVMEGITLIAGSSEKIGGIVTVISDIADQTNLLAL
                     NASIEAARAGEHGRGFAVVADEVSKLADRSSASTKEIEALIRESARNVTQGVQTAKGS
                     QTAMEQIRGASAKVKEMIGQLSGAMAQQVDSVQELSKALGSVSQMSQSISAATEEQTS
                     TAKQVSKAVESVNEVTQSAASAAEEMSAATEQLATMAQELQTLTSQFKIDDSAASPAI
                     P"
     gene            8933..>9901
                     /locus_tag="VFH83_07935"
     CDS             8933..>9901
                     /locus_tag="VFH83_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6486334.1"
                     /translation="MRDIESTIKGGSPAGSGGDRRARPGTSLASRSGWAHLGRQLWKY
                     RFFHLLVLPGFLYYVIFHYLPMVGVVIAFTDYRGMGGIHGILTAPWVGFKNFVDLFQS
                     HFFWRLLRNSLILSGLRLFFGFPAPIILALLVNEVRMRKVQRVVQTITYLPHFLSWVV
                     IAGLVSMLLTSTGPVNAALVSLFHIEPILFLSDPKYFRGVLVTSGIWQSIGWNSIIYF
                     AAIAGVPQEQYEVAIVEGASRGQRAWYVTLPWMRFVIVILLVLSIGSIIESDFEQIFN
                     LYNPAVYEVSDVFDTYVYRRGLQDRDFSYATAVGLFKSVVSFGLVMV"
BASE COUNT         1639 a         3327 c         3127 g         1808 t
ORIGIN      
        1 gggtcccgaa tccgggggag acgaactgtc cggcgttcag cgactcgacc gtatcgcaca
       61 gatcgaagct gacggtcatg ggatcagcgg gcggcggcgc ccacggcaga acccgccagg
      121 gagtcggcgt gcaccctgcg ataaccgcag ggggtcatgt tggtggccct cttgaagacc
      181 ctgcggaagt aggcgggatc cgcgtacccg cagagggccg ccacctccgc gacccccttc
      241 ccgcgctgga gcaggagctg cgctcgcacc tccttgatcc ggcgatcgtg gaccgcttct
      301 cggatggaga tcctgcgctc caggtgatag gcgcgctcca gatagtcggg gttgtaccgc
      361 agctccgccg cgatgtccgg ggtcccgatg ggctcgtggt agtgggccgc gatgtaggca
      421 tccacgcggg aggcaatgct ttccagcgcc gcctcccggt tctccgtccg ctccgaggcg
      481 cgcgaggaat gcgccagctc gcagagcgcg agcgccacca ggtggtggag aatggcgcgg
      541 gacggcgacg ggcggcgggt ctcatccatg agccgccgca ggagatgggt cagccgcgcc
      601 ggacggagga cggtgacatg gtccggaatc tccagccgat ggctccatgc gtcgcccgag
      661 gaaccgccga cacgggatcg ggcggcccgt gggggcggct ggaggaacga gaggatatac
      721 agctccgaag acggggaccg ctgcaggacg agctccccat ggaaggccga gaggagaaac
      781 gcttcgctgg gacacaggac gacgtcccca tgtgcgcgca aggcgcgcgc caacgggtcc
      841 gcgggggccg ccgggccgcc tcgcagcgcg atcacgcctc gagccgcgaa aaggagcagg
      901 ttctcgtttc ccttccccaa tgtcaggctg gtgactccgg cgggctcaac ggcaagatga
      961 tccgcgcgta cgccctcgaa agcgcgcgtg catccgaggc ggatacattc ctccatcgtc
     1021 gtgagtttac ctttggcaac ggccgggtca agcgacgcgc tcacggggcg gctcccttgg
     1081 tcatcgtatc gcgcgcacgg ctactcgcgc acccacacca ccatctcacc gggggtccga
     1141 ttggcccacg cataatacgg gacgaaggtc agctccttgc gatccgccct cggcgcgcca
     1201 cggttcgagt acagctcccc atcggtcgtc gtcgatcccg ccgcgccgcg ttccccggcg
     1261 tcggacaggc cggtcgtccg gatgaccgtc accccgccca gcagatcgcc gcggtgcgag
     1321 gcctccagga cggcatcctg cggaagccgg atgccggcga ggttgtcccc gttgtcggcc
     1381 tcctcgagac aatagacgat gggcccgcgc tggatcgcca cccgcccccg gttgccggct
     1441 acccgagggt ccgccctcat cctctggatg gtgagcggca gcgtcagctc gacggtgtcg
     1501 ccggccgacc agcgtctgcg caccctggcg tacccgtcgg cgtcggcccc tgccgcgacg
     1561 gcggcgccgt tgacctggag cggcgccccc acgcaccagg acggaatccg gacggccagg
     1621 gtgaggtccg atcgcgtttc gctcagccac tggaaccgga tccgaccgtc ccagggatag
     1681 cgcgtgctct gcttcagacg gatcgtgtcg ttgccgattc ccacggtcac ggtgccttcg
     1741 tggaagaggt cggcgtacag cgcatcgccc gagcagtggt acgcatactg accgagagag
     1801 gcgagcagcc gcgcgatgtt cggcgggcag caggcgcagc cataccaggg ctggcgtcgg
     1861 tacttgacgt gccggtagcc gccgttggcg tcgcagaccg ccgggatgac ccccaagggg
     1921 ttgacgtaga agtagctctc accgtccagc gccagcccgc tcaggatccc gttgtgcagc
     1981 gcccgctcca tgacgtccgc gtacttcgcc tggctgcgca gccgcaccat ccgggccgaa
     2041 aagaagaaca gaccgatggc ggcgcaggtc tcggcgtagg cgatgtcgtt cgggaggtcg
     2101 aagtcagcgc cgaaagattc cagatgggcg gaggacccga tcccgcccgt gatgtacatc
     2161 ttccgccgcg tgacgctctg ccacagacgc tcgcaggcct ccgccaggga agggtcgccg
     2221 gtctcctggg ccagatccgc catggccgtg tacagataca cggcgcgtac ggcatgaccc
     2281 accgcgtccg gctgctcccg gaccggcgcg tgcgcctggt agtagtccag ggatttgatc
     2341 ccgaagaagg ggtgatagcc gggcgcggcc gcctcggtct ggaagtagct cggggatcgg
     2401 ccccgctcgt cgacgaagta ggaggccagg cgcaggtacc gcgtctctcc ggtggcgcgc
     2461 gccagcttga ccagggccag ctccacctcc tcgtgcccgc agtacccacg gaccttcccc
     2521 gcctgcggtc cgaaccggga gtcgatgtgg tccgccagcc gcttcaccac ctccaggatc
     2581 cgctggttgc cggcgcccag gtacagcgag accccggcct cgatcatgtg ccccgcggag
     2641 taaagctcgt gagcctcccg aagattcgtc catcgggcgg acggctcctt cacggtgaag
     2701 tacgtgttga ggtacccgtc cggttgctgg gctcgggaga ccagatccac cgcctcgtcg
     2761 acccaggctc tgagcgccga gttgccggcg ccttcctccg ccaggagata cccggccgcc
     2821 tcaagccatt tggccagatc gctgtcctgg aacaccatcc cttcgtaggt tccggagcgg
     2881 acacccgcgg cgatccggaa gttctcgacg gaatggctct tgggggctcc ggggatctcg
     2941 tcgttcatgg ccttccattg ataaggaatg accttgttgc ggagcaggtc ctggtagcct
     3001 ttccagaatc ccccgtcgat cttcgatctg ggctgcgccg acacgggaaa tccctcgctc
     3061 atgaccttcc cccggcctca aagatccgct accggcgcga cttctgcaag tctccagagt
     3121 ccgagaaaat ggcgacaaaa ccgacctggg cccgacagcg gatccgcgcc atggaccgcg
     3181 gaccgggaag tcggaatcgt acccgttttc tacgagaggc gcaccttgcg ggtgcaccgg
     3241 gggcgcgcgt agcttatacc catcttctgg tgcagaggag gacagaacca tgatgagaag
     3301 gttgggcatg gcggccgggc tcgcgggggc gttgctgctc ctgatggcgc tcaccgccgg
     3361 cgcggacccg gtaaagatcc agtacggata ctggggagac cagaacgagg agacctcgac
     3421 caccgccctg ctgcagacgt tcatggatgc gcatccgaac atccaggtgg aaggtctgcg
     3481 gttcgggtcc aacacgcagt tcaacgacaa gatcacgacc ctggccgcgg ccggtacgct
     3541 gccggacgtg agcacgttct acgagcccaa cgttctgacg tggggcatga acgggcagtt
     3601 cgtcgacctg acggacttct acaagaaggc cgctcccaag ctggacgcca tcaagttcgt
     3661 gacgccggac ggaaagatcg tggggatcag cgtcgccaac gagatccagg tgatctggta
     3721 caacaagcgg atcttcgacg cggcgaacct cccctatcct cccgcggatc ccgccaaggc
     3781 gtggacgtgg gcccagtttg tcagtgtggc caagaagctg acccgggatt ccaacggcaa
     3841 gaccccgaac gacaaggggt tcgaccccag caacatccgg accttcgggg catgggtcca
     3901 gcagtggtgg atgccctggc tcacgttcgc cgtgagcaac ggcggcggca tggtcagcca
     3961 ggacggcaag cggctgatga tgggtgaccc ggcgaccatc gacgctatcc agaagatggc
     4021 cgacctgatc aacgtcgacc acgtgagccc ttccccgcag tcccagcaga tgccgaccgg
     4081 cgtggccaca tcccttctca gcggacaggt ggccatggcc atggacggga cgtgggacct
     4141 cctgaccgtc gggaccacca aggaccagca ggggctggac ttcgggatcg gcgtcctgcc
     4201 ctacatgaag aagctggcca cgacgtccgt gggaactccc gaagtggtct acaagagcac
     4261 ccagcacctg gatgaggcgc tggccctgct ggggtatctg atggaccccg ccagctccct
     4321 gccactgctg cagaacggga tctggctccc caacgagaag cagtggtaca cggacccgac
     4381 ccttctgcag aagtggatcg gaaacccgcg gcacccgccg gagtacaagc aggcggtcgt
     4441 gaacttcgcg ctcaattatt ccaaccgact gccgctgtac tacgttccga cgttcggccg
     4501 gatggacgac gtgatcgagg cggcgctcgg ccaggtctgg ctcgggcaga agaccgcggc
     4561 gaacgtcatc aagaacgaga tcatgccgaa gatcactccg atcttccaag gccagtagtc
     4621 ctgcagcctt ccgggcgcct ccgagggggc gcccggtttc gtccgcccta ccagggaccc
     4681 ggtcgccagg gacgggcgcg cgcagccgga gggatccgat gaagaggcga cgtcttcgcg
     4741 ccagcaccac ggaggcgata gcagggtacc tgttcgcctc tcccgtcatc ctggggctgg
     4801 tgatctgggt gatcctgccc atgttcggcg tgctggcgat cagcctgacc gactggaacg
     4861 tgctgcgcgc gccgcgttgg gtcggggtcc agaactacgt aaagctcttc acgacggatc
     4921 tgtacttctg gaactcgctg aaggtcacgg cctacttcgt cgtgctcaac gtggcgtgca
     4981 cggtcctcta ctccctggtg gtggcgctcc tgctcaacca gaagatccgg ggccgcggga
     5041 tctaccgcag cttcttctac ctgcccacga tcatcccggt ggtggcgtcc agcgccctgt
     5101 ggtcctggct gttcaacccg gagttcgggc tgctcaacgt gctcctgcag gccgtgaagc
     5161 tgcatcgatc cctgtggctc gcggcccaga ccaccgtggt cccctccatc gtcctgatga
     5221 ccgtctgggc cggcgcgggc aacaccatcg tgatcttcct ggcggccttg cagggagtgc
     5281 ctcgcgagct gctggaggcg gtggagatcg acggcggcac ctggtggcat cggttccgcg
     5341 ccatcacgct ccccatgatc tccccggtga ccttcttcaa cgtcgtcacg ggtctgatcg
     5401 cctcctttac catcttcacc cagtcctaca tcatgaccaa cggcggtccc aacaacgcca
     5461 gcctcttcta cgtgctgctg ctctatcggg aggcgttcca gcgcaacaat tttggcggcg
     5521 cggcggcgct ttcctgggtc ctgatcatca tcgtgtccgt cctcagcctg ttgatcttcc
     5581 ggacctccag ccggtgggtc tattacgaag gagaggcgcg gtgagactga gcgttgcccg
     5641 cgtcaccggc cgcggcgtgg tgtacctcct gctcaccata ggcgccgcct tctgcatcct
     5701 gccggtcttc tggctggtcc gaagctccgc gatgaccctg ggggacatct tcaagttccc
     5761 gcccatcctc tggcccgcca ccatccggtg gcagaacttc gcggatgcca tgaaggcaag
     5821 cccgttcctg cgctacttcc gcaacacgat gatcgtcgtg gtcccgaacg tggcaggggc
     5881 gctcctcacg gcgagcatgt ccgcctacgg tcttgcgcgg ttcaggttcc cggggcgcaa
     5941 tttctggttc tccctggtgc tcggggccat catcctcccg cccgcggtcg tgctgatccc
     6001 gcagttcctc ctctggagca agctccacgg ggtgaacacg ttcctgcccc tgatcgtccc
     6061 ggtctggttc ggcggcggcg ccttcaacgt gttcctcctg cggcagttct tcctcacggt
     6121 cccgcgggac ttcgacgacg cggcgcgcat ggacggcgcc acctactggc gcacgtactg
     6181 gcagatcatg atcccgttca tccagccggc cctgctggcc gtcgggctct tccagttcct
     6241 cttcgcctgg aacgacttct tcaaccccct gatctacctc agctccaagg caacctacac
     6301 gcttgccgtg gggctgcaat tcttcgtcgg aagctacggc acgttctgga acatcctcat
     6361 ggccgccgcc gtagtcatcg tcgtgccgcc ggtgatcgtg ttcctcatcg ggcagcgcta
     6421 cttcgtggag ggcgtcaccc tcaccggcat caagggatag ccggcgacgc ggccgagtcg
     6481 tcgatcttga actgcgacgt aagcgtctga agctcctggg ccatggtggc aagctgctcc
     6541 gtagcggccg acatttcttc cgccgcggat gccgccgatt gcgtcacctc gttgacgctt
     6601 tccacggcct tggagacctg cttcgccgtg gaggtctgct cttcggtggc ggcggatatg
     6661 ctctggctca tctggctcac gctgccgagc gccttggaga gctcctgcac ggagtccacc
     6721 tgctgggcca tcgctccgga gagctggccg atcatctcct ttaccttggc cgaggccccg
     6781 cggatctgct ccatggccgt ctgcgagccc ttggcggtct ggacgccctg ggtgacgttg
     6841 cgggcgctct cacggatcaa cgcctcgatc tctttcgtgg aggcggagct gcggtccgcg
     6901 agcttgctca cctcatccgc caccacggcg aatccgcggc cgtgctcgcc ggcgcgtgcc
     6961 gcctcaatgg aagcattcag cgccaggagg ttcgtctggt ccgcgatgtc ggagatgacc
     7021 gtgacgatcc cgccgatctt ctccgagctg ccggcgatga gggtgatccc ttccatcacc
     7081 tcggagacgg ccttggcccc ttccagggcg ttctccaggg acctcccgga gaggctggca
     7141 atctcggtca ggtgccggga aacatcttcg atagactggt ggacctgggc catggatgcc
     7201 gagccctgtt ccgccgcagc cgcctgggtc tgagcgtgct cggccacctg gtccacggag
     7261 gcgctgagct cctccacgga tgcgctcgtc tcttcgaggg tcgaggcctc attctgggcg
     7321 ccttccgcga gcttctgcgc gctggcggag atctcctcgc tggaagaggc cacctgcacc
     7381 gcgttttcct ggatcgtgga caccatcgcc ctgagcttcc ccgacatgga gttgagggcg
     7441 gccgcgagct tcccgatctc gtcgtttcgc tggatggcga gccgctgcgt gaggtctccc
     7501 gaggcgacga cctccgcaaa agccacgccc ttctccagag gtcgagagac gctgcgcgca
     7561 aagacgaggt agacgagaaa cgccgcggca agggcggcag ccccgatcac caggatcagg
     7621 ttcctcagat cccgggaggg ggccagatac tccacgtccg ggagcgtgta tcctacgctc
     7681 catcccgtct ccgtgaccgg cgcgtaaccc gcggtcttgg caacgccgtt gaacacatag
     7741 gtgctgacgc cgctctctcc cgcgatcatc ttgcgggtga agtcccgggt gccgtccagc
     7801 gatgcaaggt tggtcttgaa gacgttgtca gccaccggat gagcgatgat gagcccgtca
     7861 tggtcgacca cgtacgcgta accggacctc ccgatcctct cgttgatgat gaggtcgttc
     7921 aggaacccgg cctcgaggat ggacacatag acgcccacca cctcgttgcc tgagcggatg
     7981 ggggccgcga ccggcaccac gggcttgccg gtgaccttgc tgatgacggc cgctccggcg
     8041 tttgcattgc ccgcgagcgc ctgtttgatg tagttgcggt ccgcgaagct cacgccaacg
     8101 taggatgggt ccgtggcggc aaacgccacg ccgtcccttc cgacgcacag gatcgtctcg
     8161 aacccttctc ccaggccctt ctgattcttc accgccagga gccggtcggt ggcgcgggca
     8221 atcgcgtctc ctcccagggc catgcctcgc ggcgccaccg ccgcggccac gatctggtcc
     8281 tggagcgcct gacccatggc gaacttcttc tcctggacga agacgtcgtt gatcgaagcc
     8341 gccacgaggc gggctgagcg accgagctgc tcggtctcga tcgcgctgat tgccagactg
     8401 gaccgctgta cggccaggta ggcgaccacc gcgagcggta tgaccagcag gatcgtcccg
     8461 acgatgatga gccttgcgcc gaacctcagg tttttgaaca taggccacct cccgatccac
     8521 atcttgacat gaacgtcaat ctggacgcgg agccttcatg cctcgcgtca ctgaagcaga
     8581 acaatgggct gctaaatggc aaacaacttc cgtgccagct caaagacgga gtgttggttg
     8641 cctctctgga gagccgctga ggccacccaa gcgtccctcc gggagctcca ggagcagttc
     8701 agcaccgcag cttgtcgctc cgcggcgcaa cgtcggggga tagccgttta gtcctgaagg
     8761 ttcgttgaaa gagacgcaac tccttgatca caaaggatta gggcgccgtc cggcccattc
     8821 tcatcaagag acctttagta ccacgccggt actccggtac cttcccgtcg cgccggaaag
     8881 tgaactcggt actcttgtac ccacaatggc cggaggatag gatgcggccc tcatgcggga
     8941 catagaaagc acgatcaagg gcggtagccc cgccgggagc ggcggggacc ggcgcgccag
     9001 gccgggcacc tcccttgcgt cgagatcggg ttgggcacac ctcggtcggc agctatggaa
     9061 ataccggttc ttccacctcc tcgtcctccc cggattcctc tactacgtga tcttccacta
     9121 tctgcccatg gtgggtgtgg tcatcgcgtt caccgactac cggggcatgg ggggaatcca
     9181 cggcatcttg accgccccct gggtcgggtt caagaacttc gtggatctgt tccagagcca
     9241 cttcttctgg aggctcctgc gcaactccct gatcctctcg gggttgcgcc tcttcttcgg
     9301 tttccccgcg cccatcatcc tggccctcct ggtcaacgag gtccggatga ggaaggtcca
     9361 gcgggtcgtc cagaccatca cctacctgcc gcacttcctt tcgtgggtcg tcatcgccgg
     9421 gttggtgtcc atgctcctca cctccacggg ccccgtgaac gcggcgctgg tctccctgtt
     9481 ccacatcgag cccatcctgt ttctttccga ccccaagtac ttccggggag tcctggtgac
     9541 ctccgggatc tggcagagca tcggctggaa ctcgatcatc tacttcgccg cgatcgcggg
     9601 cgtcccccag gagcagtacg aggtagccat cgtggaggga gcctcccggg gtcagcgggc
     9661 ctggtacgtg acgctgccct ggatgcgctt cgtgatcgtg atcctcctgg tcctgagcat
     9721 cgggagcatc atcgagagcg acttcgagca gatcttcaac ctctacaatc cggccgtata
     9781 cgaggtgtcg gatgtattcg acacctacgt gtatcggcga ggactccagg accgggactt
     9841 ctcctacgcc acggccgtcg gcctcttcaa gtccgtggtc agcttcggcc tggtcatggt
     9901 g
//
LOCUS       DASRZS010000323         4707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1716669, whole genome shotgun sequence.
ACCESSION   DASRZS010000323 DASRZS010000000
VERSION     DASRZS010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716669"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFH83_07940"
     CDS             <1..474
                     /locus_tag="VFH83_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6486335.1"
                     /translation="KDMLRYILDAGRWTTEAEELGFVARYCDLQRLRFPERLAMSITH
                     DERSAGVKIPKLLLQPVVENAVIHGIEPLGRPGRLVVEARTEGVNGSSPTRITITDDG
                     VGFLSGSGDTVERVGLANVRERLRLAYPDARMSIESSPGQGTRISIEIVDGRSGS"
     gene            471..1967
                     /locus_tag="VFH83_07945"
     CDS             471..1967
                     /locus_tag="VFH83_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6486336.1"
                     /translation="MRILIADDEQLVRFSLRNMLEEMGTPARSVLSVCDGQEMVELVR
                     RVKPEVGFVDIRMPRLDGLAAIEAARASSPATRWVILTSHSSFDYARRALQLGVSDYL
                     LKPVSPEELRKVLDKVSTEVRQELARQNDEFRGRVSSLLHGTLSLEAEEVEFVSTARF
                     AGCLIVFDSALPEDRLAERQRAVLSDLRSRIAGAAERTARLALVTLPVGYAALVCAWQ
                     PGPEEPMAAEAMHGFLHTAQTVLDGGARPDARVTLIATGTCHSFDELLSRLNRASAAA
                     PLRIPLGIGRRIDLEEAEREQAAGHWADLCAGLTGVALAVRARSRLDFLAGLETAERA
                     LEAPSTPAAERERAAVRKFTEAAVGLPPEAEPGSAEWKDRMRAAAEALGPEERAAGEL
                     VRQVREFVHGAYMRDIGIGQIAFHLGVTPNYLSSLFHRETGTTFVRFLTGLRMDHARE
                     LLAAPGARVQDVARSVGYASVRHFSRLFQRQFGAHPSDVKAEKNARES"
     gene            2045..3058
                     /gene="rhaS"
                     /locus_tag="VFH83_07950"
     CDS             2045..3058
                     /gene="rhaS"
                     /locus_tag="VFH83_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6486337.1"
                     /translation="MKLHRLVVISLVALLLGAGLVFAGGTSEQPKGNLKVALVVKNLG
                     NSFFEAVRDGGLEAAKELGGVDVIYQGPSTPTAEGQIDIINSLIAQKVNAIAISANDV
                     DALVPITKKAMAAGIKVISFDSGIAPDGRILHLAPSGDEFIGRSQVKMLADLIGDSGE
                     FAILSASSQATNQNAWIDWMKKELPNHPNMKLDAVVYGDDLSDKSYREAMGLFKSYPN
                     LKGIIAPTTVGIAAAGKALEDAGLAGKIQLTGLGLPSEMKQYIKDGTCLKMSLWNPID
                     LGYSATYIAVQLVRGKIKGTPGEAMSVGRMGTINIDDKGTAVMSEPYVFNKDNIDKFA
                     AIY"
     gene            3142..4641
                     /locus_tag="VFH83_07955"
     CDS             3142..4641
                     /locus_tag="VFH83_07955"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6486338.1"
                     /translation="MTDPLLECRTISKSFPGIRALDEVDFTLLPGEVHALVGENGAGK
                     STLVKILTGIYRPDSGTISLSGHPVEFRSPVAAQAAGIAAIHQEATMFSDLTVTENIY
                     MGHHQRMTGFPLLSWGAMRRRTRALLAELELDIDPDLQVRSLSVAQRHMVEIVKALSL
                     EARIVIMDEPTSALTLREVEDLFAIVRRLRDAGKAVVFISHKFEDIYGIADRFTVLRD
                     GRLAGQGLVRETGVDQIIRMMIGRSLTQMYPKIPVEPGEVLLNVRGLSRAGVFRDVSF
                     ELRRGEILGFFGLVGAGRSEVMRAVFGVDRADAGSVRIDGREVTTADPRSAMASGIAY
                     VPEDRQVQGAILAMNIRENTTLAVLDRLSNGSWLNLSRERALTDEYGARMEIKAGSWE
                     QSVNSLSGGNQQKVVLAKWLATQPRVLILDEPTKGIDVATKARVHEFITHLAKDGLGI
                     ILVSSEMPEILGMSDSIVVMHEGRVTARFRRDEADQEKLLKAAMGRMGS"
BASE COUNT          809 a         1517 c         1624 g          757 t
ORIGIN      
        1 aaggacatgc tgcgctacat cctggatgcg ggccggtgga ccaccgaggc ggaggagctg
       61 gggttcgtgg cgcgctactg cgacctgcag cggctgcgct ttcccgagcg ccttgccatg
      121 agcatcaccc acgacgagag gtccgcgggc gtgaagatcc ccaagctcct gctgcagccc
      181 gtggtggaga acgccgtgat ccacggtatc gagccgctgg gccgcccggg gcgcctcgtg
      241 gtggaagcgc ggaccgaggg cgtcaacggg agctcgccga cgcgtatcac gatcaccgac
      301 gacggcgtag ggttcctgtc gggaagcggc gataccgtcg agagggtcgg gctggcgaac
      361 gtccgagagc ggcttcggct ggcctacccc gacgcgcgga tgtccatcga gagcagtccc
      421 ggccagggca cccgcatctc catcgagatc gtcgacgggc ggagcggatc gtgaggatcc
      481 tgatcgccga cgacgagcag ctcgtccgtt tcagcctgcg gaacatgctg gaagagatgg
      541 gcactcccgc tcgctccgtg ctgtcggtct gcgacggcca ggagatggtg gagctggtgc
      601 gccgcgtcaa acccgaagta ggtttcgtgg acatccggat gccgcggctg gacggtctgg
      661 cggccatcga ggcggcccgg gcaagctctc ccgccacgcg gtgggtcatc ctcaccagcc
      721 actcgtcgtt cgactacgcc cgacgtgcgc tgcagctcgg cgtctcggac tacctcctca
      781 aaccggtcag ccccgaggag ctgcgcaagg tgctggacaa ggtctccacg gaggtccgac
      841 aggagctggc gcggcagaac gacgagttcc gcggccgggt gagctcgctc ctgcacggga
      901 ccctctccct ggaggccgag gaggtggagt tcgtttccac ggcgaggttc gcggggtgcc
      961 tgatcgtctt cgacagcgcg ctccccgagg accggctggc ggaacggcag cgcgccgtgc
     1021 tttcggatct gcggtcgcgg attgccggag ccgcggaacg aacggcacgg ctggccctgg
     1081 tcacgctgcc ggttggctac gcggcccttg tttgcgcctg gcagcccggg cctgaggagc
     1141 ccatggcagc cgaggccatg cacggcttcc tccacacggc ccaaacggtg ctggatggcg
     1201 gcgcccggcc cgacgcacgg gtgaccctca tcgccacggg gacctgccat tccttcgacg
     1261 agctcctgtc ccggctcaat cgcgccagtg ccgccgcccc tctgcgtatt cccctgggga
     1321 tcggtcgccg gatcgacctg gaagaggcgg agcgcgaaca ggccgcggga cactgggccg
     1381 atctctgcgc cggactgacc ggggtggcgc tcgccgtccg cgccaggagc cggctggact
     1441 tccttgcggg gctcgagacc gcggaacgcg cgctggaggc gccttcgacg cccgcggccg
     1501 agagggagcg ggccgccgtc cgcaagttca cggaggcagc ggtcggtttg ccgcctgagg
     1561 ccgagccggg cagcgccgag tggaaggacc gaatgcgcgc cgcggcggag gcgctgggcc
     1621 ccgaggagcg ggcagcggga gagctggtcc gtcaggtccg agagttcgtc cacggagcct
     1681 acatgcgcga catagggatc ggccagatcg cgttccacct gggggtcaca ccgaactacc
     1741 tgagctcgct gtttcaccgg gagaccggga ccaccttcgt gcgctttctc acgggcctgc
     1801 gcatggacca cgcgcgggag ctcctggcgg cgcccggggc gcgcgtccag gatgttgccc
     1861 gttccgtggg gtatgccagc gtccggcatt tcagccgcct gttccagcgg cagttcggag
     1921 cccatccctc cgacgtcaaa gcggagaaaa acgcccggga atcgtaggtt tgtagcattg
     1981 gacctcctgt tcggcgggac taccatccac tcgatttgaa ctcatctcgt tcaaggaggt
     2041 ctgaatgaag ctccatagat tggtcgtgat cagcctcgtt gcccttcttc tgggcgcggg
     2101 gctcgtgttc gccggcggca cctcggagca gccgaagggc aacctcaagg tagccctcgt
     2161 cgtgaagaac ctggggaact ccttcttcga agccgttcgg gacggggggt tggaggcagc
     2221 caaggagctg ggcggcgtgg acgtcatcta ccagggtccc agcacgccga cggccgaggg
     2281 ccagatcgac atcatcaact ccctcatcgc tcagaaagtg aatgccatcg ccatctcggc
     2341 gaacgacgtg gacgccctcg tgcccatcac caagaaggcg atggcggcgg ggatcaaggt
     2401 catctccttc gactcgggga tcgcgccgga cgggcggatc ctccacctcg ccccgtcggg
     2461 ggacgagttc attggacgca gccaggtcaa gatgctggcc gacctcatcg gcgacagcgg
     2521 agagttcgcc atcctgagcg cctcttccca ggccaccaac cagaacgcat ggatcgactg
     2581 gatgaagaag gagctgccca accaccccaa catgaagctc gacgcggtgg tgtacggcga
     2641 tgacctctcg gacaagagct atcgcgaggc catgggcctc ttcaagtcct acccgaacct
     2701 gaagggcatc atcgccccga cgacggtcgg gatcgcggcc gcgggcaagg cgctggagga
     2761 cgccgggctg gccggcaaga tccagctcac gggcctggga ctgccgtcgg agatgaagca
     2821 gtacatcaaa gacgggacct gtctgaagat gtccctctgg aaccccatcg accttggcta
     2881 ctccgccacc tacatcgcgg tgcaactggt ccgcggcaag atcaagggca cgccggggga
     2941 ggccatgtcc gtgggccgca tgggcaccat caatatcgat gacaagggca ccgcggtcat
     3001 gagcgagccc tacgtcttca acaaggacaa catcgacaag ttcgcggcga tctactagaa
     3061 tagccgcgtg tatcggtcga tcgatctcac cggaaagcgg ccgccagcgg cggccgcttt
     3121 ccatactcga caggcggtgc cgtgacggat cccctcctgg aatgccgaac catcagcaag
     3181 tccttccccg gaatccgggc gctggacgag gtggacttca cgctcctccc cggtgaggtg
     3241 cacgccctgg tcggggagaa cggggccggc aagtccacgc tggtgaagat tctgaccggg
     3301 atctaccggc cggattcggg aaccatctcg ctgagcgggc accccgtgga gttccgcagt
     3361 cccgttgccg cgcaggccgc gggcatcgct gccatccacc aggaagccac catgttctcc
     3421 gatctcacgg tgacggagaa catctacatg gggcaccacc agaggatgac cggcttcccc
     3481 ctcctctcct ggggcgcgat gcgacgcagg acccgggcgc tcctggcgga gctggagctg
     3541 gatatcgatc cggacctgca ggtccgctcc ctgagcgtgg ctcagcggca catggtggag
     3601 atcgtcaagg cgctctcgct ggaagcgcgc atcgtcatca tggacgagcc cacctcggcg
     3661 ctcaccctcc gggaggtcga ggacctgttc gccatcgtcc gccgccttcg ggatgcgggc
     3721 aaggctgtgg tcttcatttc ccacaagttc gaggacatct acggcatcgc ggaccgcttc
     3781 accgtgctgc gggacggacg gctggcagga cagggcctgg tgcgggagac cggcgtggac
     3841 cagatcatcc gcatgatgat cgggcgatcc ctgacgcaga tgtacccgaa gatcccggtg
     3901 gagccggggg aggttctgct gaacgtaaga ggcctgtccc gggcgggcgt gttccgggac
     3961 gtcagcttcg agctgcgtcg aggagagatc ctcggcttct ttggcctcgt tggcgcgggg
     4021 cgctcggagg tgatgcgcgc cgtgtttggc gtggaccgcg cggatgccgg ctccgttcgg
     4081 atcgacggcc gggaggtcac cacggcggac cctcgctccg ccatggcaag cgggattgcc
     4141 tacgtgccgg aggatcggca ggtccagggc gccatcctcg ccatgaacat ccgagagaac
     4201 acgacgctgg cggtgctgga ccggctctcc aacggctcct ggctgaacct ctcgcgggaa
     4261 cgcgccctca cggatgagta cggggctcgc atggagatca aggccggaag ctgggagcag
     4321 tcggtgaaca gcctctccgg cggcaatcag cagaaggtgg tgctggccaa gtggctggcc
     4381 acccaaccac gggtcctgat cctggacgag ccgaccaagg gcatcgacgt ggcaaccaag
     4441 gctcgcgtgc acgagttcat cacccacctg gccaaggatg gcctggggat catcctggtg
     4501 tcttcggaga tgccggagat cctgggcatg tcggattcca ttgtggtcat gcacgagggg
     4561 cgggtcaccg ctcgcttccg cagggacgag gcggaccagg agaaactgct gaaggccgcc
     4621 atggggagga tggggtcgtg agggccgcgg gatgaaccgt cttctgcggc gcagggaaag
     4681 caccctggtg atcatcatcc tcgtgct
//
LOCUS       DASRZS010000324         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1718704, whole genome shotgun sequence.
ACCESSION   DASRZS010000324 DASRZS010000000
VERSION     DASRZS010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718704"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /gene="buk"
                     /locus_tag="VFH83_07960"
     CDS             <1..958
                     /gene="buk"
                     /locus_tag="VFH83_07960"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002834.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HET6486339.1"
                     /translation="LPDQLEYRGHAVRTGVRESGIPLSDIAAVVGRGGLLKPLPGGVY
                     EVNERMKADLLSCRYGVHASNLGGLIADSLAAELGVKAFIVDPVVVDELDALARRSGI
                     PEIPRRSIFHALNQKATAKKAARSLGKPYGGCVLIVAHMGGGTSVGLHVDGRVVDVNN
                     ALDGDGPLAIERAGTVPAGDWMRWVLSHQHDPQALQLMLTGRGGLVAYVGSNDFAALE
                     RIAAAMDDQKALAADELIRTLCYQVAKAIAALAAFTGGRVDGVVLTGGLAYSTRVVSE
                     VTDRVSFLAPVLVFPGENELEALAQGAREVLLGEAKALTYEG"
     gene            967..2028
                     /locus_tag="VFH83_07965"
     CDS             967..2028
                     /locus_tag="VFH83_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET6486340.1"
                     /translation="MTQSSVTDERALPPFARKYLGSKRLLRGWIADQITDRVGVPRSF
                     LDGFFGTASISYEMALRGVDRIVAVEALRSNCVVLRGSCAARTGAQSGERLDEVRRKL
                     NALEPVRGYVVQSYADRYFTEKNCCRMDAIREEMERLLNAGEITIGEHEQLLASFILE
                     ADRVANTIGQYDAFLKNPHGPSSVGGRHLVDDRVQSPFRLRTLAPFPGRNVEVIEGDL
                     VDLAPGIAVEVAYYDPPYNTRQYCDNYHVLENLARWEKPRLYGKTRKFERSGMKSPFS
                     RRRDAAAALERLIMGTRAAYLFLSYSSEGILDPETIALILSRRGQVQVSQRPYPVFGN
                     GAGVSRRRNVVEYLFFVTG"
     gene            2033..>2389
                     /locus_tag="VFH83_07970"
     CDS             2033..>2389
                     /locus_tag="VFH83_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007143494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HET6486341.1"
                     /translation="MAGTKRELIDDNGRSRGFYSEENRVNDLTGKEWVFWTRSVITRP
                     YPPNLQHALRSRHGGQKPPDLCADLLKVLTKAGQTVLDPFMGVGGVLLGASLCGRAAL
                     GVEINPEWVGIYDRVCE"
BASE COUNT          404 a          749 c          819 g          417 t
ORIGIN      
        1 actcccggat cagctcgagt accgggggca cgcggtccga acgggtgtgc gggaatccgg
       61 tatacctctg tctgatatag cggcggtcgt gggtcgcgga ggtctcctga aaccgcttcc
      121 cggcggcgtc tacgaggtca acgagaggat gaaggccgac ctcttgagct gtcgctacgg
      181 cgtccatgcc tccaacctcg gggggctgat cgccgactct ctggccgcgg agctcggtgt
      241 caaggccttc atcgtggatc ccgtggtggt ggacgagctg gacgcgctgg cgcgtcgctc
      301 cggcatcccc gagatccctc gtcgttccat cttccacgcc ctgaaccaga aggcaaccgc
      361 gaagaaggcg gcccgcagcc tggggaagcc ttacggcggc tgcgtgctga tcgtcgcaca
      421 catgggcggc gggacgtcgg tagggctcca cgtggatggc cgcgtggtgg acgtcaacaa
      481 cgcgctcgac ggcgacggcc cgctggccat cgagcgagcc gggaccgttc ccgccgggga
      541 ctggatgcga tgggtgctgt cccaccagca cgacccgcag gcgctgcagc tcatgctgac
      601 tgggcgcggt ggactggtgg catacgtagg ctccaacgat ttcgccgctc tggaaaggat
      661 cgccgccgca atggacgacc agaaggcgct cgcagccgat gagctcatcc gaaccctgtg
      721 ctatcaggtg gccaaggcga tcgccgccct ggcggcgttc accggcggtc gggttgacgg
      781 cgtcgtcttg accggtggac tggcgtactc gacccgtgtg gtgagcgaag tcacggaccg
      841 tgtctccttc ctggctccgg tcctggtttt cccgggggaa aacgagctgg aagccctggc
      901 ccagggcgcc cgggaggtcc ttctgggaga ggccaaggcc cttacgtacg aagggtgagg
      961 ccggccgtga cacagagctc cgtaacggac gagcgggcct tgccgccctt tgcgcgaaag
     1021 tatctcggct cgaagcgact ccttcgcggt tggatcgcgg accagatcac ggaccgagtc
     1081 ggtgtaccgc gatccttcct ggatggcttc ttcggaacgg cgtcgatcag ctacgagatg
     1141 gctttgcggg gcgtggaccg tatcgttgcc gttgaggccc tgcggtccaa ctgcgtggtc
     1201 ctccgcggat cctgtgccgc caggaccggc gcgcagtcgg gcgaacgcct cgatgaggtg
     1261 cgcagaaaac tgaacgccct ggagcctgtc cgaggctacg tggtgcagag ctacgcggat
     1321 cgctatttca cggagaaaaa ctgctgccgc atggatgcta ttcgggagga gatggaacgg
     1381 ctcctgaatg ccggtgagat caccatcggg gaacacgagc agctcctggc aagtttcatc
     1441 ctcgaagcgg accgggtagc gaacacgatc ggccagtacg atgcgtttct caagaaccca
     1501 cacggcccga gcagtgtggg gggccgccac ctcgtggacg accgggtcca gagcccgttc
     1561 cggctccgga ccctggcccc gtttccaggt cggaacgtcg aggtcatcga gggggatctc
     1621 gtcgacctgg cgccaggcat cgccgtggag gtggcctact acgacccgcc gtacaacacc
     1681 cgtcagtatt gcgacaacta ccacgtgctg gagaacctgg cccggtggga gaagccgagg
     1741 ctatacggaa agacccgcaa gttcgagcgc tccggcatga agagcccctt ctcccggcgc
     1801 cgagacgccg ccgctgccct ggagcggctg atcatgggca cccgcgccgc ctacctgttt
     1861 ctctcgtaca gctccgaggg gatcctggat ccagagacga tagcgctgat cctttcccgt
     1921 cggggccagg tccaggtgtc gcaacggccg tatccggttt tcggcaacgg cgccggagtg
     1981 tctcgccgtc ggaacgtggt ggagtacctg ttcttcgtga cggggtagcg atgtggcggg
     2041 aaccaagaga gagctgatcg acgacaacgg gcgctcccga ggcttctact cggaggagaa
     2101 ccgagtcaat gatctcaccg gcaaggagtg ggtcttctgg actcggtcgg tgatcacccg
     2161 gccctatccg cccaatcttc agcacgcgct gcgcagccgt catggcgggc agaaacctcc
     2221 ggatctctgc gcggacctgc tgaaggtgct caccaaggcc ggccagaccg tgctggatcc
     2281 cttcatgggc gtgggcgggg tcttgctggg cgcctccctc tgcgggcgcg ccgccctggg
     2341 cgtggagatc aaccccgagt gggtcgggat ctacgaccgg gtgtgcgag
//
LOCUS       DASRZS010000325         3650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4414276, whole genome shotgun sequence.
ACCESSION   DASRZS010000325 DASRZS010000000
VERSION     DASRZS010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4414276"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VFH83_07975"
     CDS             <1..962
                     /locus_tag="VFH83_07975"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET6486342.1"
                     /translation="GGSRAVKYGTVRRFVLGLQVVLASGEVLELGGKFIKSSAGYDLL
                     NLMVGSEGTLGVITRVTLGLLPPVGSVQTVVVPFRTVEEAIGAVPAMFASGILPTAVE
                     FVGHEVLRCSERLLNRSWPTHDGSASLMVILDGRNENDTLEQAQAISDRLEKAGAIDV
                     LITDQKARQAEILELRSMLYEAIRPVTVEVFDVCIPRSEIAAHVGFVAGLEKRVGLAI
                     PTYGHAADGNVHSHCLKSPMVDGAFGPEVAGWKEKVHEIEREIYADAVRRKGTISGEH
                     GIGLAKRAFLPLSVGPAHLAAMKAIKKALDPLGILNPGKIFEA"
     gene            984..1694
                     /locus_tag="VFH83_07980"
     CDS             984..1694
                     /locus_tag="VFH83_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486343.1"
                     /translation="MAEQAPDGPESLERLLSMLEAFYGKQRAIGPTDAFGMVIYRNCG
                     YPPSEERCAAGYASLKRAIGLTPHQILEAPKGRLTEAMRPGGMMPAGRAERLQDIALS
                     VTTEYGGNLGSILELPLVQARKALKRFPTIGDPTADKILLFTGTAALAALPSNCLHVL
                     LRLGFGQERRSWAASYRLAQAAVSAQVPETLRARRRAYLLLKRHGEELCKLNQPRCLE
                     CPVSAHCRYYLEAARDPA"
     gene            1730..3286
                     /locus_tag="VFH83_07985"
     CDS             1730..3286
                     /locus_tag="VFH83_07985"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6486344.1"
                     /translation="MRSGVRLEGGRKSTFFPWFLVNTVILSLLLALNLLMRYRPLTAL
                     PVTSLQTLAIVGLGVCLLADGLMAYAFSRANHRRISEVARQMVRLAGIRTGNGNQYTI
                     LREAVRATLSWKAKLDLDLCQGTAAMRQDFLRRLARGHWTGAAEVERAFAQFGIVPLS
                     SCYAVFVVRPRSPLDRVTPAHDTGRKLASFIIASAVEAILNRLQCGFVAEHDGSLVCI
                     VSLRLLSALEWNRDLDTIIADTRTYLQETFGLRTIVGVSTLVNGVQRIPQAFREALSA
                     LEYGPVVEPESVIRYGDVRNRQSTCRRSLETDEQLGNAVRLGDEEKTAEILRLIMDQN
                     LRGADVSIQSLKCFIFDMYNLLSQAIATVDEPRQRELARAIQPLMRALEGDSSLAALE
                     AEVQGVVLAVCGSLRSVRKDNGLALEIRRHVEANYTDLNLSIGAIADRFDLSQGYLTR
                     LFRERTGQSLLDFITGVRISHAKELISKQHRSLEDVAGAVGYLSASALIRAFKRHEGV
                     APGRYRYAGG"
BASE COUNT          642 a         1158 c         1236 g          614 t
ORIGIN      
        1 ccggcggatc gcgcgcggtc aagtacggca cggtgcggcg cttcgtgctg ggtctccagg
       61 tggtgcttgc cagcggcgag gtcctggagc tgggcggcaa gttcatcaag tcgagcgcgg
      121 gctacgacct tctgaacctc atggtgggct ccgagggcac cctgggagtg atcacgcgcg
      181 tgaccctggg cctcctgccg ccggtaggat ccgtgcagac cgtggtcgta ccgtttcgga
      241 ccgtggagga ggccatcggc gcggtccccg cgatgttcgc ctcgggcatc ctgcccaccg
      301 ccgtggagtt cgtggggcac gaggtcctgc gctgctccga gcggctgctc aacaggagct
      361 ggcccacgca cgacggaagc gcgtccctca tggtcatcct ggacgggaga aacgagaacg
      421 acacgctgga gcaggcccag gccatcagcg atcgcctgga gaaggcgggc gccatcgacg
      481 tactgatcac ggaccagaag gcccgtcagg cggagatcct ggagctgcga agcatgctct
      541 acgaggccat ccgtccggtc accgtggagg tattcgacgt ctgcatccct cgaagcgaga
      601 ttgcggcgca cgtcgggttc gtggccggcc tcgagaaacg agtgggcctc gcaatcccta
      661 cctatggcca cgcggcggat gggaacgtgc acagccactg tctcaagtct ccgatggtgg
      721 atggcgcctt cggcccggag gtcgccggct ggaaggagaa ggtccacgag atcgagagag
      781 agatctacgc ggacgccgtg cgccgcaagg ggaccatctc cggagagcac ggcatcgggc
      841 tggccaaacg cgccttcctg cccctgagcg taggcccggc gcacctcgcc gccatgaaag
      901 ccatcaagaa ggccctggat ccgctgggga tcctcaaccc ggggaagatc ttcgaggcgt
      961 aggcctgggc cctggagcgc gccgtggcag agcaagcgcc ggacggaccg gaaagcctcg
     1021 aacggctcct ctcgatgctc gaagcgttct acggaaagca gcgagcgatc ggccccacag
     1081 atgcgttcgg catggtgatc taccgcaact gcggctatcc cccatcggag gagcgctgcg
     1141 ccgccggtta cgcgtccctg aaacgtgcaa tcgggctgac ccctcaccag atcctcgagg
     1201 cgccgaaagg caggttgaca gaggcaatgc ggcccggagg catgatgccg gcgggacgag
     1261 ccgagcggct ccaggacatc gcgctttcgg tgaccacgga gtacggcggc aacctgggct
     1321 ccatcctgga gttgccgctg gtccaggctc gcaaggcgct gaagaggttc ccgaccatcg
     1381 gcgatcccac ggcggacaag atcttgctct ttacgggaac cgccgctttg gcggcgctgc
     1441 catccaactg cctccatgtc ctcttgcgcc tgggcttcgg ccaggagcgc aggagctggg
     1501 cggcatccta tcggcttgcc caggcggcgg tgagcgccca ggtgccggag acgctgaggg
     1561 cacgccgccg cgcgtacttg cttctgaagc ggcacggcga agagctctgc aagctcaacc
     1621 aaccgaggtg cctggagtgt cccgtgtccg cccactgtcg gtactacctt gaagccgcga
     1681 gggatccggc gtagtatcgg tgttccggta ggccggaggc gaaaaagtca tgcgttctgg
     1741 tgtccgtctc gaaggcggac gaaagagcac cttcttcccg tggttcctcg tgaacaccgt
     1801 gattctgtcg ctgcttctcg cgctgaatct cctgatgcgc tatcggcccc tcaccgcgct
     1861 ccccgtgacc tcgctgcaga cgctcgcgat agtcgggctg ggggtatgcc tcctggccga
     1921 cgggctcatg gcgtacgcct tctcccgcgc aaaccaccgc cggatctccg aggttgcgcg
     1981 gcagatggtc cgcctggcgg ggatcagaac ggggaacggg aaccagtaca ccatccttcg
     2041 ggaggccgtc agggctacgc tgagctggaa ggccaagctg gacttggacc tctgccaggg
     2101 caccgccgcc atgcgccagg acttcctgcg tcggttggcc cgggggcact ggacgggcgc
     2161 agcggaggtc gagagggcgt tcgcccaatt cgggattgta cccctgtcct cgtgctacgc
     2221 ggttttcgtg gttcggcccc gaagcccgct ggaccgcgtc acaccggcgc acgacaccgg
     2281 ccgcaagctt gcctccttca tcatcgccag cgccgtagag gcgatcctca accggctcca
     2341 atgcggcttc gtcgcggagc acgacggcag tctggtttgc atcgtcagcc ttcgcctcct
     2401 ctcggcgctg gagtggaacc gggacctcga caccatcatc gccgacaccc ggacctacct
     2461 ccaggagact ttcggcctgc ggaccatcgt cggcgtgagc accctggtca acggcgtcca
     2521 gcgcatcccc caggccttcc gggaggcgct gagcgcgctg gagtacgggc ccgtcgtgga
     2581 gccggagagc gtcatccggt atggggacgt gcggaaccgg cagtcgacct gccgccgttc
     2641 gttggagacg gatgagcagc tcggcaacgc ggtgcggctg ggagacgagg agaagaccgc
     2701 ggagatcctg aggctcatca tggatcagaa cctccgcggc gcggatgtgt cgatccagag
     2761 cctgaagtgc ttcatctttg acatgtacaa cctgctctcg caggccatcg ccacggtcga
     2821 tgagccgcgg cagcgcgagc tcgcgcgcgc cattcagccc ctgatgcgcg cgctggaagg
     2881 ggattcctcc ctggccgcgc tggaggccga ggtccagggt gtggtgctcg cggtctgcgg
     2941 ctccctgcga agcgtccgaa aggacaacgg actggcgctc gagatccgac ggcacgtgga
     3001 ggccaactac acggacctga acctgagcat cggcgcgatc gcggatcgct tcgacctgag
     3061 ccagggctat ctcacgaggc tcttccgcga gcggacgggg cagagcctcc tggatttcat
     3121 caccggggtc cggatctccc acgccaagga gctcatctcc aagcagcacc gatccctgga
     3181 ggacgtggcc ggagcggtcg gctacctgag cgcgagcgcc ctcatccgag ccttcaagag
     3241 gcacgagggg gtggcccccg ggcgctatcg atacgcgggc gggtaaaggg atacgccggc
     3301 cttgaaagac ggtttggccg caccggtttc gtcatattga ccgtggcaga attgcgttgt
     3361 cagatggtca acgttggccc ttgagcttga tcactggata gggggcggcc tatgcgaatc
     3421 cggcttgaaa tcgtaggacc cgtccgtgaa gcagatatcg agttcgggga tctgaccatc
     3481 cttgtcgggc agcaggcaac tggcagtgca ttactctgca gttcgcgaag atgatgaccg
     3541 acaccggtca gattcaggcg gaccttggca agcacggaat gaactgggag agaaagcgta
     3601 gcaagtcctt tgacgtgtac ttcggggagg ggatggacca ggtctggaac
//
LOCUS       DASRZS010000326         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_178669, whole genome shotgun sequence.
ACCESSION   DASRZS010000326 DASRZS010000000
VERSION     DASRZS010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178669"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="VFH83_07990"
     CDS             complement(<1..1409)
                     /locus_tag="VFH83_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486345.1"
                     /translation="MRKVTVRVGFVPSFRFRWTPWCEKMRTDSLAAFSRVRGLEVVVP
                     QPGPQGTELDAERGWSPHGMVASLDQADAMADYFAAQKVDALILCPLDFGDERSAAKV
                     AERLRVPVLLYATKEPPAPDSPDLGRVSDSYCGNLSMASGLYRRKIPFHYAGIFFPEE
                     PAFQQEVASFASAVAVVKGLRGARLGQVGVRPPTFETVGYDETALIQKFGQTVIYREL
                     GDVVRAAQELRENDPRVQAALQRLRASAAQITISTTAMLKTAQLEAALADLWTENRLS
                     ALAVQCWPSLYAEMGVSMCALQGRLTGESMLTSCEADVLGAIAMLVSYQSVIGETVPH
                     FIDWTIQHRQDPNRLLAWHCGNAPSCLARDPAQTALRSRRDMTGKLPVIEDDPQAGLA
                     QFQIKPGRVTFNRLVEYDNQWKMLIASGEIVPSEETLAGTWAWVQVRDHARLYRTLVE
                     EGFVHHASMIYGDQTRQLEL"
     gene            1619..>2875
                     /locus_tag="VFH83_07995"
     CDS             1619..>2875
                     /locus_tag="VFH83_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486346.1"
                     /translation="MKRLVLALCVVLLASGGMLFASGKTEGAASGGPAPQLTVQLMDN
                     EQPDVPAVTAEINKILLKNLNVTVKFQHLTWTDWQQKYKLLVTSGEPVDLLYAANWND
                     YATYARNGAYKQLDDMLSKDAPELLKAIPKTSWDGVKIQGKIWAVPPLRYGYSGTGHL
                     LYRADLQKKWGVAPVTDMASFTAYLDAVKKNAPDMIPCEDPGWDWVGSLLLNNITKDN
                     PGHTWFTAFINQSQLFWYDYKNQKPDNIKAIYEFPEYKQFLQTMHEYAGRGYWAQDVL
                     AEKSIASEIFEAGKTASTVGGAGTQNIDKTQELIQRVAKNNPTWEVGELSWDKYRGFA
                     IPAAAQQDLTTVPIQSKYPDLALKVTSAMLLNKDLQYLIDYGIKGTHYNVSDKGEYVQ
                     LPGAKNYMLFGMAAWAWKNQSLFLADG"
BASE COUNT          546 a          958 c          909 g          462 t
ORIGIN      
        1 agctcgagct ggcgggtctg gtccccgtag atcatgctgg catgatgcac gaacccttct
       61 tcgaccaggg tccggtacag tctggcatga tcgcgcacct ggacccacgc ccaggtaccg
      121 gcaagcgtct cctcggaggg gacgatctct ccggatgcga tcagcatctt ccactggttg
      181 tcgtattcca ccaggcggtt gaaggtcacg cggccgggct tgatctggaa ctgcgcgagg
      241 cccgcctgag gatcgtcttc gatgaccggg agcttgccgg tcatgtcccg gcgggagcgg
      301 agcgcggtct gggcggggtc ccgggccaga cagctcggcg catttccgca gtgccaggcc
      361 aggagccggt tgggatcctg acggtgctgg atggtccagt cgatgaagtg cggcacggtt
      421 tcgccgatca ccgactggta gctcaccagc atggcgatgg cgcccaggac atccgcctcg
      481 cacgatgtaa gcatgctctc cccggtcagt cgtccctgga gcgcgcacat cgagacgccc
      541 atttccgcgt agagcgaggg ccagcactgc accgccagcg ccgacagccg gttctccgtc
      601 cacaggtccg ccagggccgc ctcgagctgg gcggtcttga gcatcgccgt ggtcgagatg
      661 gtgatctggg ccgcgctcgc tcgaagccgc tgcagggccg cctgaacccg cggatcgttc
      721 tcccgaagct cctgtgcggc gcgcaccacg tcccccagct cccggtagat caccgtctgc
      781 ccgaacttct ggatgagcgc cgtttcgtcg tagcccaccg tctcgaaggt gggcggccgg
      841 acccccacct gaccgaggcg cgcgccgcgc aggcccttga ccaccgccac ggccgacgcg
      901 aacgacgcga cctcctgctg gaacgcgggc tcttccggga agaagatgcc ggcgtagtgg
      961 aacgggatct tgcggcgata caggcccgaa gccatggaca ggttgccgca gtaggagtcg
     1021 gaaacgcgcc ccaggtccgg gctatccggc gccggcggct ccttggtggc gtagagcagg
     1081 accggcacgc gcagccgctc cgccactttg gccgccgacc tctcgtctcc gaaatcgagc
     1141 gggcagagga tcagcgcgtc gaccttctgg gcggcgaagt agtccgccat ggcgtccgcc
     1201 tggtccagac tggccaccat gccatgcggt gaccatcccc tctccgcgtc cagctccgtg
     1261 ccttgcggcc ctggctgtgg aaccaccacc tcgagccctc gcacccggga gaaggcggcc
     1321 aggctgtcgg ttcgcatctt ctcgcaccac ggcgtccagc ggaaacggaa cgatggcacg
     1381 aacccgaccc ggacggtgac cttgcgcatg gagctcccct tggcggatgg attcgaaggc
     1441 accggcggac ggtgtctcgg tgacccagta tacggcgtct tgaaaaaact tgacagatcc
     1501 gggaggccgg acttatagtt ctgcggaacc aagcccatcc aaggacaggc tccgaagatt
     1561 cacatcgcgc ccagcgccgg ggccggaccc tgagcgctgg caggaaagga gtcaggaaat
     1621 gaagagactg gtcctcgctc tctgcgtcgt ccttctcgcc agcggcggca tgctgttcgc
     1681 ctccggcaag acggagggcg ccgcttcagg gggccccgcg ccacagctca cggtgcagct
     1741 gatggacaac gagcagccgg atgtccccgc ggtcaccgcg gagatcaaca agatcctgtt
     1801 gaagaacctg aacgtcacgg tgaagttcca gcatctcacc tggaccgact ggcagcagaa
     1861 gtacaagctg ctggtgacgt ccggggagcc ggtggatctc ctgtacgccg ccaactggaa
     1921 tgactatgcg acgtacgccc gcaacggcgc ctacaagcag ctcgatgaca tgctttccaa
     1981 ggacgcgccg gagcttctca aggccatccc caagacgagc tgggacggcg tgaagatcca
     2041 gggcaagatc tgggcggtgc cgccgctgcg ctacggctac tcgggaacgg gccacctcct
     2101 gtatcgcgca gacctgcaga agaagtgggg cgtggcgccg gtgacggata tggcttcctt
     2161 cacggcctac ctggatgcgg tcaagaagaa cgcgccggac atgatcccct gcgaagatcc
     2221 cggatgggac tgggtcgggt cgctccttct gaacaacatc accaaggaca acccgggcca
     2281 cacctggttc accgcgttca tcaaccagag ccagctcttc tggtacgact acaagaacca
     2341 gaagccggac aacatcaagg ccatctacga gttccccgag tacaagcagt tcctccagac
     2401 catgcatgag tacgcgggcc ggggttactg ggcccaggac gtcctggcgg agaagagcat
     2461 cgcctcggag atcttcgagg ccggcaagac cgcttccacg gtcggcggcg cgggcaccca
     2521 gaacatcgac aagacccagg agctgatcca gcgcgtggcc aagaacaacc ccacctggga
     2581 ggtgggtgag ctgagctggg acaagtaccg cgggttcgcc attccggccg ccgcccagca
     2641 ggacctgacc acggtcccca tccagtccaa gtatcccgat ctggccctca aggtcacctc
     2701 ggccatgctc ctgaacaagg accttcagta cctcatcgac tacggcatca agggcacgca
     2761 ctacaacgtc agcgacaagg gtgagtacgt ccagctcccc ggcgccaaga actacatgct
     2821 cttcggcatg gccgcctggg cctggaagaa tcagagcctc ttcctcgcgg atgga
//
LOCUS       DASRZS010000327         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4415271, whole genome shotgun sequence.
ACCESSION   DASRZS010000327 DASRZS010000000
VERSION     DASRZS010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4415271"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VFH83_08000"
     CDS             complement(<1..675)
                     /locus_tag="VFH83_08000"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HET6486347.1"
                     /translation="MSVPGLRRAVCALLFLAASGLAAQTPGTVTGVDRLGSRTALQID
                     QGGIPLFETRPIPRMTYSVDLYRIRFLSEDFDGSSAEITAQLFVPVVPSGEGCPVLVF
                     GSGTTGISDACAPSRENPQEHPWGYYRENMLAYAGLGFITIFPDYLGFNDPTRPQRYF
                     SKLAEAHVMLDAARAVRAFLASEDGITRAAPQVFLAGYSQGGHAAFSAADLWSSYAPE
                     LPLVGVM"
     gene            complement(672..1916)
                     /locus_tag="VFH83_08005"
     CDS             complement(672..1916)
                     /locus_tag="VFH83_08005"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HET6486348.1"
                     /translation="MLHKPYGFDTRRRTRFLVIAFLAAVALAAPAQPKVQILTSQLVE
                     TLTPAAIDRLEASLFTADPAPKARLSVDSWLITFQSLYPDGTPATILAQLFIPRFADR
                     AVRPLYVFATGSTGLTSNCRPSREHVVGIHWGLYRTHVLAFAAQGAVGMLPDYMGFAD
                     TGHLQPFYSAVAEGRMMLDSIRAARTFLGQQAAKGVADTAAFTAGFSQGGHAAFAAAD
                     LRSSYAPDVKLRGVIGYGPATDVVTLFREFPVDAPMVIYTYAALYGKARFDPAAILAP
                     KWAETLQDDVTRQCIGGMQAYYPWTPRELFRKEFADALLAGTLPKAYPGINAVLTQNS
                     SGLSGHRVPALILEGTDDVVVSVPSQTAFARALSARGSPVQLSIYKGVRHDTRQAAFG
                     EALKWMTTITQGGKPPSNGGSL"
     gene            2035..2670
                     /locus_tag="VFH83_08010"
     CDS             2035..2670
                     /locus_tag="VFH83_08010"
                     /inference="COORDINATES: protein motif:HMM:NF022630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042121 - alginic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate O-acetyltransferase AlgF"
                     /protein_id="HET6486349.1"
                     /translation="MRASLFVAAVLAFGVTSSLAAAPGSVYGPSAPGDSAFVRVVNAL
                     KSVPSLRADLGATRFQDVSYGAASAYLPVAPDVYLVRVAGLELEVIAKTRTYYTVAFT
                     SGGILLFEDATHTDPARAQLFLYNLTSIDPIDLKTSDGKTTVIKGVRPGTSGVKVVNA
                     VSATLAVYSGGTRVGAPAVLALKRGSSFGVFVLGESARPTVFSVQAEVKVP"
BASE COUNT          465 a          907 c          954 g          456 t
ORIGIN      
        1 catcacaccg accagcggca gctctggggc gtaggacgac cagaggtccg cggcggagaa
       61 cgcggcgtgt ccgccctggg agtagcccgc caggaagacc tggggggctg ctcgagtgat
      121 cccgtcttct gatgccagaa aggcgcgcac ggcccgggcc gcgtccagca tgacgtgagc
      181 ctccgccagc ttgctgaagt atcgctgggg tcgcgttggg tcgttgaacc ccaggtagtc
      241 cgggaagatc gtgatgaaac ccagtcccgc gtatgccagc atgttctcgc ggtagtatcc
      301 ccaggggtgc tcctgagggt tctcccgcga aggcgcgcag gcatccgaga tgccggtggt
      361 accagagccg aagaccagca cggggcagcc ctcccccgag gggacgaccg gcacgaagag
      421 ctgggcagtg atctccgccg aggatccatc gaagtcctcg ctgagaaatc ggatccggta
      481 caggtccacg ctgtaggtca ttcggggtat gggccgtgtc tcgaatagcg ggatgccccc
      541 ctggtcgatc tgcagggcgg tccgggaccc caggcggtcc acccccgtga cggtgccggg
      601 agtctgggcc gcgaggccgc tcgcggcaag gaagagaaga gcgcagacgg cgcgtcgaag
      661 gccagggacg ctcacaacga acccccgttg gatggcggtt ttccgccctg ggtgatggtg
      721 gtcatccact tcagggcttc cccgaacgcg gcctgtcgcg tgtcgtgtcg gactcccttg
      781 tagatgctga gctggaccgg gcttccccgg gcgctcagcg ccctggcgaa ggccgtctgg
      841 gaggggacgg ataccaccac atcgtccgtg ccctccagga tcagggcggg aacgcggtgc
      901 cccgagagcc cgctcgagtt ctgggtcaaa acagcgttga tccccgggta ggccttgggc
      961 agggtccccg ccagaagcgc gtccgcgaac tccttgcgga agagctcgcg cggcgtccag
     1021 ggatagtatg cctgcatgcc tccgatgcac tggcgcgtga cgtcgtcctg gagcgtctcg
     1081 gcccacttcg gagccaggat ggcggcggga tcgaaccggg ccttcccgta gagtgcggcg
     1141 tacgtataga tcaccatggg cgcatcgacc gggaactccc gaaacagggt gaccacgtcc
     1201 gtggcgggac cgtagccgat cacacctcgg agcttcacgt ccggcgcata gctggagcgc
     1261 agatccgcgg ccgcgaacgc ggcgtgaccg ccctgggaga aacccgccgt gaaggccgcc
     1321 gtgtccgcaa cgccctttgc ggcctgctgg cccaggaagg tccgtgcagc ccggatcgag
     1381 tccagcatca tgcggccctc cgccacggcg ctgtagaagg gctgcaggtg ccccgtgtcg
     1441 gcgaaaccca tgtagtccgg cagcatgccg acggcgccct gggcggcgaa ggcgagcacg
     1501 tgagtccggt agaggcccca gtggattccc acgacgtgct cacgggaagg gcggcagttg
     1561 ctcgtgaggc ccgtggaccc ggtggcaaag acgtacaagg gccggaccgc ccgatcggcg
     1621 aaccggggaa tgaaaagctg ggcaaggatg gttgccgggg tgccatcggg atagaggctc
     1681 tgaaacgtga tgagccagct atccaccgaa aggcgagcct tgggtgccgg gtcggccgta
     1741 aagagggacg cctccaacct gtcaatggcc gcgggggtga gggtctccac gagctgggag
     1801 gtgaggattt ggaccttggg ctgcgcgggc gccgccaagg ccacggcagc gagaaaagcg
     1861 atcaccagga atcgggtccg gcgacgcgtg tcgaaaccat acggtttgtg gagcatgaga
     1921 acctgcctcg aagagtgaac ggacaacagt agcgccacga gagccgagcg tcaacgaccg
     1981 tcccgtctcg acaccccgcc gtgggcgggc tacaatggta cctaaaggat ccccatgcga
     2041 gcctctctct tcgttgccgc cgtattggct ttcggcgtca cctcttcgct ggcggcggcg
     2101 cccgggtcgg tctacggccc ctccgctccc ggcgattccg cgttcgtgcg cgtggtcaat
     2161 gcgctgaagt ccgtcccatc cctccgcgcc gacctggggg ccacccggtt ccaggatgtc
     2221 tcctacggag ccgcgtccgc gtacctgccg gtggcgcccg acgtatacct ggtgagggtc
     2281 gcggggctgg agctggaagt gatcgccaag acccggacct actacaccgt ggccttcacg
     2341 tccgggggta tcctcctctt cgaggatgct acgcacacgg accccgcgcg ggcgcagctc
     2401 ttcctctaca acctcacctc gatcgatccc atcgacttga agacctcgga cgggaagacc
     2461 accgtcatca aaggcgtgcg gcccgggacc tctggcgtga aggtcgtgaa tgcggtgagc
     2521 gcgaccctcg ccgtgtacag cggcgggact cgtgtcggcg cccccgccgt cctggcgctg
     2581 aagcgcggct cctcgttcgg cgtcttcgtg ctcggagagt ccgccaggcc gacggtcttc
     2641 tccgtccagg cggaagtcaa ggtcccgtag accttcatgg cctttgccag tgggctgttc
     2701 ctgttcctgg ttctcccgat cttcctggcg gtctactacc tgcttccgcc cgccgcgcgc
     2761 cgaccctggg tcctcgtgac ga
//
LOCUS       DASRZS010000328         4005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2494852, whole genome shotgun sequence.
ACCESSION   DASRZS010000328 DASRZS010000000
VERSION     DASRZS010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2494852"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VFH83_08015"
     CDS             <1..723
                     /locus_tag="VFH83_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6486350.1"
                     /translation="PRVFTLRNYEVVFENPLLLRSFFITVARTVVGSLTHVLFTAVVA
                     YGLAKKDLMFRRGYMIFGIITLYFGGGLIPFYFVLKYLGLINRFAVFIVPGLYSVFDA
                     LLFIAYFRGIPDSLGESARIDGANEVFILARIILPISMPIVATIALFAGVGHWNAWFD
                     SAFFTTNENLRTLQLVLWQVINQAANQEFLKQRMIVKQADFTIEAVRYATMMVAVGPI
                     IFVYPFLQRYFIKGMLVGAIKG"
     gene            778..2409
                     /locus_tag="VFH83_08020"
     CDS             778..2409
                     /locus_tag="VFH83_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486351.1"
                     /translation="MKAQKLLVGMAVFLLVAGLSFAAGGKEAATAGAASTTPITLGMF
                     ANISWYNWPEWGSNEVSKAITQKTGVTIKFTWPAQGDDQQFNLMLASGDLPDFILNDQ
                     QDPLFEKPIQAGLVEDLGPLMDKYAPDLRKRMGDGYWKFNAYQDGKNYAIINAEMHPG
                     NIKRYIPVGPWNPGTFYLEDVYKAVGSPKIDTPDDLFTTLKTVQAKYPDLRPLWIGAS
                     PLNFTPQPYGYDYWFAQFGVEKYYDQNGQLSAKYKDPKYLDALLWLNKLYHQGLIKRE
                     DLASTDDQYRSEHDAGKIFYATTGIGNFNPETGYESVPANPTVKYVLGPMFAPATILQ
                     QGSIGWCGWMITTKAKDKARAIQFMAFSMSQQGEELQTFGIPTENWSYGSNGLPVRSA
                     SYLSAVKNNTIADYNKQWGVGAYWFNLDLYDHVMVLSDQLASPNWQKVLKMYVPHANM
                     KMWHLNLRPVSDSPEAVILAKATKLYTDTFPSFILADGPEQVKANLAAFIKQADALGM
                     PQVEALWTKQSLAAKAIWKGVDLVTDQEKFTKALK"
     gene            2498..>4005
                     /locus_tag="VFH83_08025"
     CDS             2498..>4005
                     /locus_tag="VFH83_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486352.1"
                     /translation="MDITGNWTDEQGYLPHIRTREPMPVEGTETRFSQTLDLPPGSYR
                     LELLYDPLQDKRPGWSTGTGSFRGFSLNGSRLPLRFVPAPDGRTAAASVGLTVPGGRT
                     ELGLRLPNTRPLRTLLHPAQAMPTSGRFLAVRGGGGNGETVICHGVPFLMRRLDIRHI
                     PPFIRHRDRVVEDRGSLAPWSAGVRFDCGGARPRLAHFLGMIHNLDIANGSWYSPKGD
                     HGYSHFVGDRAGEIVIRWDDVGETRIPLVFGYNLWYGKAWDNLWHYLDYAPGRGGANF
                     DATLFDGHDEHRRAIEAGLALADGYRLMGSDSCNARFIFTVDLGGRSVRSIDVAAVPE
                     LYDYPLISAITFETTGDAGSPPAALAAVPDLVGGATGLRPVTLDSIERETWRAGVEGI
                     QRVLYTFVDDLPRLQAPSKPDDYFGPTYDFRGSQIATYAATYLYWNGPECSAYIADDG
                     TGCSSSTADLALVHYTQGMGAWYQRRSLFGSIENWLRLYREREPGGLPGSGEA"
BASE COUNT          727 a         1327 c         1284 g          667 t
ORIGIN      
        1 ccccgcgtct tcaccctgcg caactacgag gtggtcttcg agaacccgct cctcctgagg
       61 tcctttttca tcacggtggc gcggaccgtg gtgggttccc tgacgcacgt gctcttcacg
      121 gccgtggtgg cgtatggcct ggccaagaag gacctgatgt tccgtcgggg atacatgatc
      181 ttcgggatca tcacgctgta cttcggcggc gggctcatcc cgttctactt cgtcctgaag
      241 tatctggggc tcatcaaccg gttcgccgtg ttcatcgttc cggggctgta cagcgtgttc
      301 gacgcgctgc tcttcatcgc atacttccgg gggatcccgg actccctggg ggagtcggcg
      361 cggatcgacg gggccaacga ggtcttcatc ctggcccgga tcatcctgcc catctccatg
      421 cccatcgtgg ccaccatcgc gctgttcgcc ggcgtgggcc actggaacgc ctggttcgac
      481 tcggcgttct tcacgacgaa cgagaacctg cgcacgctgc agctcgtcct ctggcaggtc
      541 atcaaccagg ccgccaacca ggagttcctc aagcagcgga tgatcgtgaa gcaggcggac
      601 ttcacgatcg aggcggtccg ctacgccacc atgatggtgg ccgtggggcc gatcattttc
      661 gtctacccct tcctgcagcg ctacttcatc aaggggatgc tggtgggcgc catcaaggga
      721 tagaggtccg gagaagacag ggcgttgccc tgaggtagta ctctacagga ggttccaatg
      781 aaagcgcaga agctgcttgt ggggatggcg gtctttctgc tggtggcggg cctgagcttc
      841 gccgccggcg ggaaggaagc cgccaccgcg ggggccgcgt ccacgacccc catcacgctg
      901 gggatgttcg ccaacatcag ctggtacaac tggccggagt ggggttccaa cgaggtgtcc
      961 aaggccatca cccagaagac cggcgtgacc atcaagttca cgtggcccgc ccaaggggac
     1021 gatcagcagt tcaacctgat gctggcctcc ggggacctgc cggacttcat cctgaacgac
     1081 cagcaggacc cgctgttcga gaaacccatc caggcggggc tggtggaaga cctgggtccc
     1141 ctgatggaca agtacgcgcc ggacctccgc aagcgcatgg gggacgggta ctggaagttc
     1201 aacgcgtacc aggacgggaa aaactacgcg atcatcaacg cggagatgca cccgggaaac
     1261 atcaagcggt acatcccggt cgggccgtgg aaccccggca ccttctacct ggaagacgtc
     1321 tacaaggccg ttggctcgcc gaagatcgac acgccggatg acctgttcac caccctgaag
     1381 accgtccagg cgaagtaccc cgacctgcgg ccgctgtgga tcggggcctc cccgctgaac
     1441 ttcacgccgc agccctacgg gtacgactac tggttcgccc agttcggcgt ggagaagtac
     1501 tacgaccaga acggtcagct cagcgccaag tacaaggacc cgaagtacct ggacgccttg
     1561 ctctggctca acaagctcta ccatcagggg ctgatcaagc gggaagacct ggcctccacg
     1621 gacgaccagt accggtccga gcacgacgcc ggcaagatct tctacgccac gaccggcatc
     1681 ggcaacttca acccggagac gggctacgag tcggtgccgg cgaaccccac cgtgaagtac
     1741 gtcctgggac ccatgttcgc tccggcaacg atcctccagc agggatccat cggctggtgc
     1801 ggctggatga tcaccaccaa ggccaaggac aaggcccgcg ccatccagtt catggccttc
     1861 tccatgagcc agcagggcga ggagctgcag accttcggca tccccacgga gaactggagc
     1921 tacggctcca acggcctgcc ggtccgttcc gcctcctatc tgtcggccgt caagaacaac
     1981 accatcgccg actacaacaa gcagtggggg gtcggggcgt actggttcaa cctggacctc
     2041 tacgaccacg tgatggtcct gagcgaccag cttgccagcc ccaactggca gaaggtcctc
     2101 aagatgtacg tgccgcacgc caacatgaag atgtggcacc tcaacctgag gcccgtctcc
     2161 gactcgccgg aggccgtcat cctggccaag gccaccaagc tctacacgga caccttcccc
     2221 tccttcatcc tggcggacgg ccccgagcag gtgaaggcca acctcgcggc gttcatcaag
     2281 caggcggacg cgctgggcat gccgcaggtg gaggcgctct ggaccaagca gagcctggcc
     2341 gcaaaggcca tctggaaggg cgtggacctg gtgaccgacc aggagaagtt caccaaggcg
     2401 ctcaagtagc ggatcccaga cgcggccctg ccgttggggc gcgccgccgt tgcggggacc
     2461 gggccgatcc ggtccccgtt tttgtacgga ggggaacgtg gacatcaccg gaaactggac
     2521 cgatgagcag ggatacctgc ctcacatccg aacccgggag ccgatgccgg tggaggggac
     2581 ggagacgcgg ttctcccaga ccctggacct tccgcccgga agctaccggc tggagcttct
     2641 ctacgatccc ctccaggaca agcggcccgg atggtccacg ggaaccggct cgttccgagg
     2701 tttcagcctg aacgggtccc ggctgccgct tcggttcgtg cccgccccgg atggccgaac
     2761 cgcggcggcc agtgttgggc tcaccgtccc gggagggcgc accgagctcg gtctcaggct
     2821 gccgaacacg aggccgctgc gaacgctcct gcaccccgct caggcgatgc cgacgagcgg
     2881 acgcttcctg gcggtacgag gaggcggggg caacggcgag acggtgatct gccacggcgt
     2941 gccgttcctg atgcgccggc tggacatccg ccacatcccg ccgttcatcc ggcacaggga
     3001 ccgtgtggtg gaggaccgcg gatccctggc gccgtggtcc gctggcgtgc ggttcgactg
     3061 cggaggcgcg cggcctcgcc tcgcccattt tctcggcatg atccacaacc tcgacatcgc
     3121 caacgggtcc tggtatagcc ccaagggtga ccacggctac tcgcacttcg tgggtgaccg
     3181 ggccggtgag atcgtcatcc ggtgggacga cgtcggagag acgcggattc cgctcgtgtt
     3241 cgggtacaac ctctggtacg gcaaggcatg ggacaacctg tggcactacc tggactacgc
     3301 gccgggccgg ggcggcgcca acttcgacgc cacgctgttc gacggtcacg acgagcaccg
     3361 acgcgccatc gaagccggac tggcccttgc ggacgggtat cgcctgatgg gatcggatag
     3421 ctgcaacgcc cggttcatct tcacggtcga cctgggcggg agaagcgtgc ggagcatcga
     3481 tgtggcggcg gtgccggagc tttatgacta cccgctcatc tccgcaatca cgttcgagac
     3541 gaccggcgac gcaggttccc ctccggcggc cctggccgcc gtgccggacc tggttggcgg
     3601 cgccacgggg ctccgacccg tcaccctgga cagcatcgag cgcgagacct ggcgcgccgg
     3661 tgtggaaggc atccagcgag tcctctacac cttcgtggac gatctcccca gactgcaagc
     3721 gccctcgaag cctgacgact acttcgggcc gacctacgat ttccgtggca gccagatcgc
     3781 tacctatgcg gccacctacc tctactggaa cgggccggag tgctcggcct acatcgccga
     3841 cgacgggacc ggctgctcct cttccacggc ggacctggcc ctggtccact acacccaggg
     3901 gatgggggcg tggtaccagc ggaggtcgct cttcgggagc atcgagaact ggctgcggct
     3961 ctaccgggag cgggagccgg gcggactgcc gggaagcgga gaggc
//
LOCUS       DASRZS010000329         4224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3645330, whole genome shotgun sequence.
ACCESSION   DASRZS010000329 DASRZS010000000
VERSION     DASRZS010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3645330"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VFH83_08030"
     CDS             complement(<1..957)
                     /locus_tag="VFH83_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6486353.1"
                     /translation="MPSLPTGTITFLFTDIEGSTRIWEGHPGEMRADLARHDRLIRDA
                     IEANGGCAFRHQGDSFCAAFATAPGALGGALEAQRALTEEPWAVPGGIRVRMALLTAA
                     AEEQDGDYVGPALNRIARLLSTGHGGQVLVSHSTAELVRDVLPPQTGLKPLGIHRLKD
                     LMRPEEVFQVLHPRLPSGFPPLRSLDERRHRLPLQLTPLIGREKELAAIQALLSGEGA
                     RMLTLTGPGGTGKTRLALQAAADLLDSFEDGAWFVDLSAADSSSHLVPAITRTLDVRE
                     AGARPTLELLRDLTYRKRLLLILDNVEQISGAAHHVLEILHAC"
     gene            complement(1048..1491)
                     /locus_tag="VFH83_08035"
     CDS             complement(1048..1491)
                     /locus_tag="VFH83_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HET6486354.1"
                     /translation="MMQGMSERERLVAPCGIDCGNCQLHLAAGKSELIESLVARGIPR
                     EKVPCAGCRTIDGKCPVIQGDCETYTCVTKHAVEFCYKCAEYPCGKLHPSADRATVLP
                     HNMKVYNLGVIRRDGVGKFIAVSGEIERTYFKGKMAVGRGPQLLS"
     gene            complement(1532..3082)
                     /locus_tag="VFH83_08040"
     CDS             complement(1532..3082)
                     /locus_tag="VFH83_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET6486355.1"
                     /translation="MSSSLWNASIASLKRLTPAQSSWFWLAAGVLLLPFTAWQTVVPI
                     AGWLAPIVLLRFSRTARPKWLAQRLLFLVCAGSILIGNRGMPFSLLGLIGNVVVKGLA
                     WSLVYAADRWLSGRLKGWLRTLAFPLSFATMEWALSFTPIANSGSPAYSQTGSLALLQ
                     LLSVTGLWGVTFLITWCASVANELWERGSDWRSARGLAVAFVAALLVVFLAGGARLAF
                     APSPSRTVAAATVTGTAATIEAATKPIDWLRGNWSTEAQRAALRPGFSRTVDQMLART
                     ETALEGGATLVAWQESAAWVLVEDQRAVIDRAAALARGKGAYLALSMEVLTGASKLPV
                     LRNEAILVGPAGSVLWIYDKQDPNPYDEAFATIRGRETLPLADTPFGRLSTAICYDTY
                     YPALVRQAGRRDADVLVEPANDSKPFAESAAEMATYRCIENGFSMLRPTGNGISAMID
                     RQGRFLARQVSSTDDGGVMMASLPVLGRPTIYSRIGDAFPCAGAAALAALVVATLLRR
                     KSSLDAPR"
     gene            complement(3219..>4224)
                     /locus_tag="VFH83_08045"
     CDS             complement(3219..>4224)
                     /locus_tag="VFH83_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6486356.1"
                     /translation="YLFRDPPLWKAYLDAAKRFDNDVGFELYDYGPVDVVDGSRPHTE
                     ERIVYRDAERILTQAFDTATGRWSATGIVYPAVNPPVEHVDPAQFGVEQPPRQWTPVN
                     PRSQPEGYAAWRAIREELGDHGILGMSSGFGTFVLGSASDVYSWYDDPGPWIEKIEQM
                     TARALERIDEIAKLPVRPDFLTCGGSGTLIFQTPDFVREHLLPGLKKVTRRAREVGIS
                     THIHSCGPERALVDMVVRETDLTSIDPLEIPPMGDCDLAEIKRAYGERIVLKGNLHTT
                     QVMLLGSVDDVRRASRKAIDDARTGGRFILSTGDQCGRDTPLTNIDAMVETARTYGRY
                     "
BASE COUNT          730 a         1397 c         1407 g          690 t
ORIGIN      
        1 gcatgcgtgg aggatctcca ggacgtgatg cgcggcaccg gagatctgtt cgacattgtc
       61 caggatcagg agcaggcgct tgcggtaggt aaggtcgcgc aggagctcca gggtggggcg
      121 cgctccggct tctctgacgt ccagggtacg ggtgatcgcc gggaccaggt gagatgagga
      181 atccgcggcg gacaagtcga cgaaccaggc gccgtcctcg aaagagtcca ggagatccgc
      241 cgcggcctgc agcgcaagcc gtgtcttgcc ggttcctccc gggcccgtga gcgtgagcat
      301 gcgcgcaccc tcgcccgaca gaagcgcctg gattgcggca agctccttct cccggccgat
      361 gagcggggtg agctgaaggg gaagacgatg gcgacgttcg tccagggatc gcagtggagg
      421 gaagcctgaa ggaagtcggg ggtgaaggac ctggaacacc tcttcgggac gcatcaggtc
      481 cttgagccgg tggatcccca acggcttgag gccggtctgc gggggcagca cgtcccgcac
      541 caactccgcc gtcgagtggg agaccaggac ctggcccccg tgacccgtgg agagcaatcg
      601 ggcgatccgg ttcagggccg gtccaacgta atctccatcc tgctcctccg ccgcagcggt
      661 gagcagcgcc atgcgcaccc gaatgccacc cggcacggcc cacggctctt cggtgagggc
      721 ccgctgcgcc tcgagggctc cgcccagggc gcccggcgcc gtggcaaacg ccgcgcagaa
      781 cgaatccccc tggtgccgaa acgcgcatcc accgttggcc tcgatggcat cacggatgag
      841 acggtcatgc cgggccaggt cggccctcat ctcccccggg tggccctccc agatccgggt
      901 gcttccctcg atgtcggtga agaggaaggt gatcgtaccg gttggcaggc tcggcaccga
      961 cgatctcctt gtcgaacgga ttctgaatcg gcctcagtat aggatgcccg cggtctccct
     1021 gcaatctccg ccacgccaaa cgatccgcta cgagagaagc tgaggtcccc ggcccaccgc
     1081 catcttgccc ttgaagtagg tcctctcgat ctccccggac accgcgatga acttcccgac
     1141 cccgtccctg cggatcacgc cgaggttgta gaccttcatg ttgtgcggaa ggaccgtggc
     1201 gcgatccgcc gacgggtgga gcttcccgca cgggtactcg gcgcacttgt agcagaactc
     1261 caccgcgtgc ttcgtgacgc acgtgtaggt ctcacagtct ccctggatca cgggacactt
     1321 cccgtcgatg gtccggcatc ccgcgcacgg caccttttcc cgagggatcc cgcgagccac
     1381 gagggattcg atcagctccg acttgccggc ggccaggtgg agctgacagt tcccgcagtc
     1441 gattccgcac ggcgccacca ggcgctcgcg ctccgacatc ccttgcatca tcgctgttcc
     1501 tcctgtcgta gcttggacga cagtaggccg ttcaccgcgg cgcatcaagg gagcttttcc
     1561 gtctcaggag agtcgcaacc accagcgcag ctagagcggc ggcgccggcg catgggaatg
     1621 catccccaat gcggctgtag atcgtgggcc tcccgaggac cggcagactg gccatcatca
     1681 cgcccccgtc gtccgtggac gaaacctggc gcgccaggaa gcgtccctgg cggtcgatca
     1741 tcgccgagat accgttgccg gtaggccgca gcatggagaa cccgttctcg atgcatcgat
     1801 aggtcgccat ctcagccgcg gactcggcga acggcttact gtcgttcgcg ggctccacca
     1861 gcacgtccgc atcccgtcgc ccggcctgtc ggaccagggc cggatagtag gtgtcgtaac
     1921 agatggccgt gctcagccgc ccgaagggcg tgtccgccag aggcagcgtc tcgcgcccgc
     1981 ggatcgtggc gaaggcctcg tcgtacggat tggggtcttg cttatcgtag atccacagga
     2041 ccgaaccggc cggacccacc aggatagcct cgttgcgcag cacggggagc ttggaagcgc
     2101 cggtcagcac ctccattgag agagcgaggt aagcgccctt gcccctggcc agcgcggcgg
     2161 ctcgatcgat caccgcccgc tggtcctcta ccaggaccca cgcggctgat tcctgccagg
     2221 ccacgagcgt tgccccgccc tccagggccg tctcggtacg ggcgagcatc tggtccacgg
     2281 tgcggctgaa cccaggacgc agcgcggccc gctgagcctc ggttgaccag ttgcccctca
     2341 gccagtcgat gggcttcgtg gcggcctcga tggttgccgc tgttccggtt acggtggcgg
     2401 ccgcaacggt ccgggacggc gatggggcga acgccaggcg cgcgccgccc gccaggaata
     2461 ccacgaggag tgcggcgacg aaagccacgg cgagtccgcg cgcggatctc cagtcggacc
     2521 cgcgctccca cagctcgttg gcgaccgagg cgcaccaggt gatgaggaag gtgacgcccc
     2581 acagacctgt gaccgagagg agctggagca gcgcaagact gcccgtctgg gagtacgccg
     2641 gggagcccga gttggcaatc ggcgtgaacg agagggccca ctccattgtg gcgaaggaca
     2701 gcgggaacgc gagcgttcgg agccagcctt tcagacgtcc gctgagccag cggtccgcgg
     2761 cgtagacgag ggaccacgcc aggcccttga ccaccacgtt cccgatcagc ccgagcaggc
     2821 tgaacggcat gcctcggttc ccgatcagga tggagccggc acaaacgagg aagaggagcc
     2881 gctgggcgag ccatttcggc ctcgcggtgc gagagaaccg caggagaacg atgggggcga
     2941 gccatccggc gatgggcacc accgtctgcc acgccgtgaa cggcagaaga agcaccccgg
     3001 cggccagcca gaaccaggac gactgcgcgg gggtcagccg cttcaggctc gcaatgctcg
     3061 cgttccagag agaagagctc atcaggatcc tccttgctat gcttgcaaag aggatagcgg
     3121 cctgctgttg ccggcgaatc ggtcaagcga tggattctgg ctggtcagaa acgggtagcc
     3181 cctgggcggg cgcattcacc atttctgggg gcggcggttc aatatctccc gtaggtccgc
     3241 gcggtctcca ccatggcgtc gatgttcgtc agcggggtat ccctgccgca ttggtccccc
     3301 gtggaaagga tgaaacggcc gccggtacgg gcgtcgtcga tggccttgcg gctggcccgc
     3361 cgcacgtcat cgaccgaacc gagcagcatc acctgcgtgg tgtggagatt gcccttcagg
     3421 acgattcgct cgccataggc gcgcttgatc tccgccaggt cgcagtcccc catgggagga
     3481 atctccaggg gatcgatgga ggtgagatcg gtttctcgca ccaccatgtc caccaaggct
     3541 cgctcgggcc cgcaggagtg gatgtgggtg ctgatcccca cctcgcgggc gcgtcgggtc
     3601 accttcttca agccgggcag gagatgctct cgaacgaagt ctggcgtctg gaagatgagc
     3661 gtaccggagc ctccgcaggt caggaagtcc gggcgcaccg gcagcttggc gatctcgtcg
     3721 atgcgctcca acgccctggc ggtcatctgc tcgatcttct cgatccaggg gcctggatcg
     3781 tcgtaccacg agtagacgtc gctcgcgctg cccagaacga acgtgccgaa tccgctggac
     3841 attcccaaga taccgtggtc cccgagctcc tcccgaatgg cccgccaggc agcgtatcct
     3901 tcgggttggg accgaggatt cacgggggtc cactgccggg gcggctgctc cactccgaac
     3961 tgggcggggt ccacgtgctc gacgggtgga ttgactgccg gatagacgat gccggtcgcg
     4021 gaccagcggc ccgtagccgt atcgaacgcc tgggtgagga tccgctccgc gtcccgatag
     4081 acgatccgct cctcggtgtg gggccggctt ccgtccacga catccaccgg accatagtcg
     4141 tacagctcga agcccacgtc gttgtcgaac cgcttggccg cgtccaggta ggccttccag
     4201 agcggcgggt ccctgaagag atag
//
LOCUS       DASRZS010000330         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5185218, whole genome shotgun sequence.
ACCESSION   DASRZS010000330 DASRZS010000000
VERSION     DASRZS010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5185218"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFH83_08050"
     CDS             <1..433
                     /locus_tag="VFH83_08050"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HET6486357.1"
                     /translation="ARIAVPISGSGGTYELESFSVLTGSHTPPVGAVVIRRDGRAVTQ
                     SSTGNGPIDALFRAVDKALGFATRMVLYSVSTLSAGTEAQTEVIVTVEVRGRRFHGRH
                     TSTDVIEASLRAYLRACNAVGESGILEGPSDFHVAGEYLWE"
     gene            complement(482..1666)
                     /locus_tag="VFH83_08055"
     CDS             complement(482..1666)
                     /locus_tag="VFH83_08055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6486358.1"
                     /translation="MDSGRMELSQRRAGWLSPVLFLVAAAHLFNDVYMGFLTPLYPIV
                     MQQFGLDLTRVGAVSMVAALASAFSQPLFGMAFDRFGITPALYLAPLLTGVLISLVGV
                     IPSYPLFLVLLFLGCLGSSAFHPKGASVAPTLSERPEMGMAVFSAGGNLGFAAGPVLF
                     ALFVSLYGFRSTPVLAIPAAAVGVLLFVFLPANELRRRTLTAARVTWRALFANRSDLA
                     MLVRLVFINFALNVSLRGMQTFLPVYLSSLKVSSIGIGGLFTAALALGAIASVFASSL
                     SRWTGKRLLILSSLIAGLPLALPGFLLMPSPAGVVLIILASVIQTFTNPLVIFMAQRH
                     SGGSPAMASSLIMGLSWGLAGLAMVPLGWIGELVGMKVMLGVACAVPLVALVAGVRLS
                     RD"
     gene            complement(1672..2235)
                     /gene="efp"
                     /locus_tag="VFH83_08060"
     CDS             complement(1672..2235)
                     /gene="efp"
                     /locus_tag="VFH83_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET6486359.1"
                     /translation="MIKASDIAKGTFVLINNQPHVCVEREYVNPGKGSAFVRLKLKNI
                     KTGLVLRQTNKVQDNMEDISVEYREVQFLYADAESYAFMDTKTYDQFTIPIKGEEEKR
                     DFLKDGENYQVVFWEQTPVDIKLPTKMVFTVSEAEKAERGDTVSGATKTITCETGLKV
                     KVPLFIKQGDRILVNTETGEYQERVNS"
     gene            complement(2246..>2719)
                     /locus_tag="VFH83_08065"
     CDS             complement(2246..>2719)
                     /locus_tag="VFH83_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET6486360.1"
                     /translation="AARLGLEVPGDSTWEQAFHAVFLTFVEPALPRDRPVALMDYPAA
                     VPTTARRLDGTPWSERWELYVDGVEVVNCYTEETDAMRLAELIREEANRGGASGPRPV
                     PDVALAQAFPPGFPRCSGAALGVERLEMVMAGDSSLEGVILFPFSAILRPQSSPG"
BASE COUNT          523 a          870 c          906 g          420 t
ORIGIN      
        1 cgctcggata gccgtgccga taagcggttc cggcggcacc tatgagctcg aatccttcag
       61 cgtgcttacc ggctcccaca ccccgccggt cggtgcggtg gtcatccgcc gtgacggcag
      121 ggcggtcacg cagagctcga ccggcaacgg ccccatcgat gcgctgttcc gcgccgtgga
      181 caaggccctc gggttcgcga cccggatggt cctgtattcc gtgtccacgc tctctgccgg
      241 gaccgaggcc cagacggaag tgatcgttac cgtggaggta cgcggacggc gcttccatgg
      301 ccgccacacg agcacggacg tgatcgaggc cagcctgcgc gcgtacctgc gcgcctgcaa
      361 tgccgtcggg gagtccggga tcctggaagg gccctcggac ttccacgttg cgggcgagta
      421 cctctgggag taaggcgccc catgccgccc cgggggcggg gcactgcacg gtcgacgagt
      481 atcagtccct ggagagcctg accccggcga ccaaggccac cagggggacc gcgcaggcta
      541 ctccgagcat caccttcatg cctacgagct ccccgatcca tcccaacggg accatggcaa
      601 gacccgccag tccccaggag agacccatga tcaaggagga tgccatggcg ggcgagccgc
      661 ccgagtgccg ctgcgccatg aagatcacca ggggattggt gaacgtctgg atcacgctgg
      721 cgaggatgat caggactaca ccggcgggag acggcatgag caggaacccc ggcaacgcaa
      781 gcggaagtcc ggcgatgaga gacgacagga tgaggagccg cttcccggtc caccgggaga
      841 gagagctcgc aaacaccgat gcgatcgccc ccagcgccag ggccgcggtg aaaagcccgc
      901 cgatccctat ggagctgacc ttgagcgagg agaggtagac cggaaggaac gtctgcatgc
      961 cgcgaaggct gacgttgaga gcgaagttga tgaagacgag gcgaaccagc atggccagat
     1021 ccgaccggtt cgcgaacaat gctctccacg tgacgcgggc cgccgtgagg gttcgccgcc
     1081 gaagctcgtt cgccggaagg aagacgaaca ggagcacgcc caccgcggcg gcggggatgg
     1141 ccagcaccgg cgtcgatcgg aacccgtaga gcgagacgaa gagggcgaac agcacgggtc
     1201 ccgcggcaaa cccgagattc ccgccggccg agaagacggc catgcccatc tccggccgct
     1261 cgctcagggt cggggcgacc gaggcgccct tgggatggaa tgcggaggag ccgaggcaac
     1321 ccaggaagag gagcaccagg aacaggggat acgaagggat cacgccgacc aggctgatga
     1381 ggactcccgt caggaggggg gccaagtaca gcgcgggtgt gatcccgaac cggtcgaacg
     1441 ccatcccgaa gagaggctgg ctgaatgccg aagccagagc cgctaccatg gaaacggctc
     1501 cgacccgagt caggtccagt ccgaactgct gcatgacgat ggggtagaga ggcgtcagga
     1561 accccatgta gacgtcgttg aacagatgtg ccgcagcgac gaggaacagg accggcgaca
     1621 gccaaccggc gcgccgctgg gaaagctcca tccgtccgga gtccacgtcg gctaggagtt
     1681 cacccgctcc tggtactcgc cggtttccgt gtttaccagg atccggtctc cctgcttgat
     1741 gaacaggggg actttgacct tgaggccggt ctcgcaggtg atggtcttgg tagcaccgct
     1801 gaccgtgtct ccccgttcgg ccttctccgc ctcggagacc gtgaatacca tcttggtggg
     1861 cagcttgatg tccaccggcg tctgctccca gaacacgacc tggtagttct ccccatcctt
     1921 gaggaagtct ctcttctcct cctcaccctt gatggggatc gtgaactggt cgtaggtctt
     1981 ggtgtccatg aacgcgtagc tctcggcatc cgcgtacagg aactggacct cccgatactc
     2041 gacggagatg tcctccatgt tgtcctggac cttgttggtc tggcggagga cgaggccggt
     2101 ctttatgttc ttgagcttca gccggacaaa ggcggagccc ttgcccgggt tcacatactc
     2161 gcgttcaacg cacacgtggg gctggttgtt gataagcacg aacgtcccct tggcgatgtc
     2221 gctggccttg atcatctctt ccctttcagc ctggtgagga ttgcggccgg agtatagcgg
     2281 aaaacggaaa caagatcacc ccctccaggc tcgaatcccc ggccatcacc atctccaacc
     2341 gctccacgcc gagggcggca ccggagcacc gggggaaccc gggcggaaaa gcctgagcca
     2401 gcgccacgtc ggggacgggc cgggggccgg acgcgccgcc gcggttcgcc tcttcgcgga
     2461 tcaattcggc cagacgcatg gcgtccgtct cctccgtgta gcagttcacg acctcgacgc
     2521 cgtcgacgta cagctcccag cgctcggacc acggtgtgcc gtccaacctt cgggccgtgg
     2581 tcgggacggc ggcgggatag tccatcagcg ccacgggacg gtcgcggggc agggccggtt
     2641 ccacgaaggt gagaaagacc gcgtggaagg cctgctccca ggtcgagtca ccggggactt
     2701 ccagaccgag ccgggcggc
//
LOCUS       DASRZS010000331         6381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4417400, whole genome shotgun sequence.
ACCESSION   DASRZS010000331 DASRZS010000000
VERSION     DASRZS010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4417400"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..2693)
                     /gene="mgtA"
                     /locus_tag="VFH83_08070"
     CDS             complement(87..2693)
                     /gene="mgtA"
                     /locus_tag="VFH83_08070"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HET6486361.1"
                     /translation="MTAREPASRARSAGSARALTDDASAAYCSQSPDELFAHLGSAST
                     GLSSAEADRRLRAVGWNRIEARHEVSGWTIFLSQFKSPLVLILILAAIVSAFVQEWTD
                     AAIVLVIVMASSLLSFYQEFRAAAAMEKLRGRLTNTATTLRDGEPRTIPSAQVVPGDV
                     VLLNAGSMVAADGILLEARDFYVNQAVLTGESYPVEKQPGVVREGAGLAECTNCVFMG
                     TSVQSGTARALIVRTGPQTAYGRIAERLSLRPPETEFEAGIRRFGHLLAQVVLVMTLA
                     VFSINLLLGKPRIDSLLFAISLAVGITPELLPAIISVALSKGAQQMARQGVIVRRLNS
                     IENFGSMDILCTDKTGTVTEGVVALDGALDLTGQPSAGVLRLAYLNASYQSGLPNALD
                     QAISATGRRMGLNGEHDRKVDEIPYDFQRQRLGVVTQDEEGRRCLIVKGALGRVLEVC
                     SQAHREGGMAAPLDAALKNEIQERFSAWSGQGYRVLGVAMKPVALQPNYSRADEQDLI
                     FMGFLRFFDPPKPGIQKTIADLGKLGVNLKIITGDNRLVAAHVAEIIGLKGAGTLTGQ
                     EIRAMSDEALWHRAERTTLFVEVDPNQKERIITALRRMGHVVGYMGDGINDATALHAA
                     DVGISVDTAADVAKEAADFVLLRRDLDVLRQGIEKGRTTFANTMKYVYTTTSSNFGNI
                     FSLAGASLLVPFLPLLAKQVLFQNLLSDIPNLAIADDRVDHEMVERPYRWNTRFLREF
                     MVTFGLISSLFEYVTFGALLLLLEAAPEQFRTAWFVESTLTEMLIILAMRTRTWMVKS
                     APGRLLGAATLMVAAVTIIIPYLPVSRVMGFTPLPLSILAAMVLISVLCLVTTEVTKH
                     LFYRRVHGGSPS"
     gene            complement(2690..3232)
                     /locus_tag="VFH83_08075"
     CDS             complement(2690..3232)
                     /locus_tag="VFH83_08075"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET6486362.1"
                     /translation="MVTNALTDHERPATGLEARKANGVKRGACDRKDGLAGRGGSPDA
                     DLARSAARALARSVLHPEQIRIEVDNGWVILDGEVKRPVRRAAAEHAVRWLRGVKGVF
                     NRITIGPRLVAKDLRTRIGQAVEGNAEVETGSIRITARDLTVTLRGSVGSWEEWEEAD
                     RIAWSAPGVYDVVNELIVNP"
     gene            complement(3339..5969)
                     /locus_tag="VFH83_08080"
     CDS             complement(3339..5969)
                     /locus_tag="VFH83_08080"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET6486363.1"
                     /translation="MTDSGFGSESILQTKIVRPPVRSVVHRKRLYERLESGAARKLTV
                     ISAPAGYGKTTLVSSWLGERSRDHCWVNLGQLDGSAQRVTTYLGAALDRLEQRESPPG
                     PNHWVAFLNGLAERQQDTMLIFDDYHLAESAEVNDLVMSLLENLPPTAHLVLITRIDP
                     ALRLAKLRGQGELVEIRESDLAFTIEEIRAFLTEAGGVSLTSEEALAISHTTEGWIAG
                     LHILASSLKGSADPSRLIGELSGGQRYVRDYLVEEVIARLDPSTLDFLERCSILERLS
                     ADLCDAVTGRSDSRDVLAAIDRQNLFVSPMDEEGRWFRLHHLFAGVLSARLQESHAKE
                     LPILHRKASEWFDAHHNPVEAINHRVASGDAAASAELIDRYGEWLLKRGELMTARRWI
                     GSLPDEVCAGHPLILLLRAWAAIMEGRPLEEIEKELESIGTGAFEAQVLSVRSYLAVL
                     QGKDQEALRLSKQAARIAGEPDSFVSGFAKSRVAVARLASGQVSEAIELLESTANESL
                     ETGNSLVSVVSLVHKAGALVRRGDLDGGEQAYRRALDLAAHDDGSRRWFVGWVLLGLG
                     EVHRLRGDIEGALELFREGAETSSNWLDFYDFYNILGFAQALMAKGRESEALEGLRSA
                     ERLAHKSATPLFFERLVDALWMLVLLKSRRLPEARARLSTPASRDKPGTGASYVESLV
                     SDLEILARARLALLEGENRPCIDLALPVALEARKQERGLHALYADLLLVQAHWRAEEI
                     DEATTVLERALSFASERGIVQPFVDEGPELARILYRARTLGVDHPYIGKLLAVFPLDQ
                     QSAAAVEAQPQSVEPLSAREVKVLTLLSQGLSNKEVAAKLYLSVRTVKWYTSNIYGKL
                     GVSSRTQAIAKARQLEILPE"
BASE COUNT         1123 a         2133 c         1980 g         1145 t
ORIGIN      
        1 cggcactgtg aacgctggga tggaacgctt gatgttcaca cggtcgccac gggggcgctt
       61 ttgcggcgac acttcatcgc ctgatctcac gaaggagacc ctccgtgaac ccttcgatag
      121 aagaggtgct tcgtgacttc cgtcgtcacg aggcacaaca ccgagatgag caccatggcc
      181 gccaggatcg agaggggcaa cggggtgaac cccatcactc ggctgaccgg cagatacggg
      241 atgatgatcg tgaccgccgc aaccatcagg gtcgcggctc caagcagcct cccgggagcg
      301 ctcttgacca tccaggttcg ggtccgcatg gccaggatga tgagcatctc cgtcagcgtc
      361 gactccacga accaggccgt tcgaaactgc tcgggagcgg cttccaggag gaggagcaac
      421 gcgccgaatg tgacgtactc aaagagggag ctgatcaagc cgaaggtgac catgaactcc
      481 cgaaggaaac gcgtgttcca acggtagggc ctctcgacca tctcatggtc gaccctgtcg
      541 tccgcgattg ccagattggg gatgtcggac aacaggttct ggaaaaggac ctgcttggcg
      601 agcagcggca ggaatggaac caggagggac gctccagcca gactgaatat gttgccgaag
      661 tttgagctgg tcgtcgtgta gacgtacttc atggtatttg cgaaggtggt ccgccccttc
      721 tctatcccct ggcgcagcac atccaggtcc cgccgcagca ggacgaagtc ggcggcttcc
      781 ttggccacgt ctgccgccgt gtccaccgag atgcctacat ccgcggcatg cagcgccgtg
      841 gcgtcgttga tgccgtcccc catgtagccg accacgtgcc ccatccttcg cagagccgta
      901 atgatccgct ccttctggtt ggggtccacc tccacgaaga gcgtggttcg ttccgctcga
      961 tgccacagcg cctcatccga catcgcgcgg atctcctggc cggtcagcgt tcccgccccc
     1021 ttcagcccga tgatctcggc gacgtgcgcc gcaaccagcc gattgtcgcc ggtgatgatc
     1081 ttgaggttca cccccagctt ccccaagtcc gcgatggttt tctggatacc gggctttggg
     1141 ggatcgaaga accgcaggaa ccccatgaag atcagatcct gctcgtcggc gcgagaatag
     1201 ttgggctgaa gggccaccgg tttcatcgcg acccccagca cccggtatcc ctgtccgctc
     1261 caggcggaaa accgctcctg gatctcattc ttcagcgcgg cgtccagggg cgcagccatc
     1321 cccccttccc ggtgtgcctg gctgcagact tccagcaccc tccccaaggc gcccttcacg
     1381 atcaggcagc gccgcccctc ttcgtcctgg gttacgacac cgagccgttg gcgctggaaa
     1441 tcgtaaggta tctcgtcgac tttccgatcg tgctcgccgt tcaggcccat ccgccgtccc
     1501 gtggcggaga tggcttgatc cagggcattg ggcaagccgg actggtagct ggcgttgaga
     1561 taggccagac gcaagacccc ggcggaaggc tgcccggtga ggtcgagggc gccgtccagc
     1621 gcaaccacgc cttcggtgac ggtacccgtc ttgtccgtgc acaggatgtc catgctgccg
     1681 aagttctcga tcgaattgag cctgcggacg atgaccccct gccgggccat ctgctgggcg
     1741 cctttggaca gtgcgacgct gatgatcgcc ggcagcagct ccggggtgat gccgaccgcc
     1801 agggagatcg cgaacaacag ggaatcgatg cgcggctttc ccaggagaag gttgatggaa
     1861 aagacggcga gagtcatgac cagcaccacc tgcgccagga ggtgcccaaa ccgacggatc
     1921 cccgcctcga actccgtctc tggcggtcgc agagacaggc gttcggcgat tctcccgtag
     1981 gcggtctgcg gaccggtccg cacgatcagg gctcgcgcgg tcccgctttg tacgctggtc
     2041 cccatgaaca cgcagttggt gcactcggcc agaccggctc cctcgcgaac gacgcccggt
     2101 tgcttctcga cgggatacga ttccccggtg aggacggcct ggttgacgta gaaatccctg
     2161 gcttcgagga ggatcccgtc cgccgcaacc atgctgccgg cgttgaggag caccacatcg
     2221 cccggcacga cctgcgcgct ggggatggtt cgcggctccc cgtcccgcag cgtggttgcg
     2281 gtgttcgtca gccgccctcg cagcttttcc atcgccgcgg cggcgcggaa ctcctgatag
     2341 aaactcagga gggaactggc catcacgatc acgagaacga tcgccgcgtc cgtccattcc
     2401 tgcacaaagg cggatacgat ggcggcgagg atgaggatca gcaccagtgg actcttgaac
     2461 tggctgagaa agatggtcca cccggatacc tcgtgccgcg cctcgatccg gttccagccc
     2521 actgctctca ggcgccggtc ggcttcggcg cttgacaggc ccgtggatgc gctgcccagg
     2581 tgggcgaaca gctcatccgg ggattggctg caataagcag cgctggcgtc atccgtgagc
     2641 gcccgggcac tccccgcaga acgagcacgg gacgctggtt ctcgagctgt cacgggttca
     2701 caatcagctc gttgaccacg tcatagacgc ccggcgcgct ccaggcgatt cgatcggctt
     2761 cctcccactc ctcccacgac ccaacgctcc cgcggagggt taccgtgaga tcacgcgcgg
     2821 tgatccgtat actcccagtc tccacctcgg cgttcccctc tacggcctgc ccgatcctgg
     2881 tcctcaggtc cttcgcaacc agcctcggcc cgatggtgat ccgattgaac actcccttca
     2941 cgccgcgaag ccaacgcacc gcatgctcgg cggcggctct ccggaccggc cgtttcactt
     3001 cgccatccag gatcacccag ccgttgtcta cttcgatcct gatctgctcg ggatgaagta
     3061 cggagcgggc cagcgcccgg gccgcagagc gcgccagatc cgcgtcgggg ctcccgcctc
     3121 ggcccgcaag gccatccttc cgatcgcatg ccccccgctt gaccccattc gccttcctgg
     3181 cctccagccc cgtggcaggc cgttcatggt ccgtcagtgc atttgtcacc atcgttcgat
     3241 acctcctggc atccagatgg ccccaatgta gatcggccgg ccgatcgtcc gcatcggcca
     3301 aaggtacagt cgtgggctag atgggagaac ccgacgcgtt actccggaag gatctccagc
     3361 tggcgggcct tggcgattgc ctgcgttcgg ctggagacac cgagcttccc gtagatgttg
     3421 gaggtgtacc actttaccgt ccgaaccgag agatacagct tggcggccac ttccttgttg
     3481 gatagccctt gggacaggag ggtcagcacc tttacctccc gggcgctcaa cggttcgacg
     3541 gactgcggct gcgcttcgac ggcggctgcg ctctgctgat ccagagggaa gaccgcgagc
     3601 agcttgccga tgtaggggtg gtcgactccc agggttcggg cgcgatagag gatccgggcc
     3661 agctcgggac cctcatcgac aaacggctgc acgattccgc gctcggatgc gaacgagagc
     3721 gcgcgctcca ggaccgtggt ggcctcgtcg atctcctcgg cccgccagtg ggcctgcacc
     3781 aaaagcagat ccgcatagag ggcgtggagc cctctttcct gctttcgcgc ctcaagcgcc
     3841 acgggaagcg ccaggtcgat gcacggacgg ttctcgccct cgagcagcgc cagtcgcgct
     3901 cgggccagga tctccaagtc ggagacaagc gactcaacgt aggaggctcc ggtgcccggc
     3961 ttatcccgtg aagcgggcgt cgacagtctc gcgcgggcct ccggcagccg tcggctcttt
     4021 agcagcacca acatccaaag ggcatccacc agtcgctcga agaacagggg ggtggcggac
     4081 ttgtgggcaa ggcgctcggc ggaacgcaac ccctccagcg cctccgattc ccggcccttg
     4141 gccatgaggg cttgagcgaa gccgaggatg ttgtagaagt cgtagaaatc gagccagttc
     4201 gaggaggttt cggctccctc gcggaacagt tccagcgcgc cctcaatgtc tccccgcagc
     4261 cggtggacct cccccagtcc gagaaggacc cagccgacaa accaccttcg ggagccgtcg
     4321 tcatgggccg cgaggtcgag ggcccgccga tacgcttgct caccgccgtc gagatcgccc
     4381 cgccggacca gggccccagc cttgtggacg agagatacca ccgagacaag cgagtttccc
     4441 gtttcaaggg attcgttcgc cgtcgactcc aacagctcga tagcctcgct tacttgtcca
     4501 gaggcgagcc gcgccaccgc cacccgtgac ttcgcgaatc cgcttacaaa cgagtccggt
     4561 tcaccggcta tccgggcggc ttgcttcgac agccgcaggg cctcctggtc cttgccctga
     4621 agcaccgcca ggtagctgcg cacgctcagc acctgcgcct cgaaggcccc ggttccaatc
     4681 gactccagct ccttctcgat ctcttccagc ggcctccctt ccatgatcgc cgcccaggct
     4741 cgcagcagca ggatcagcgg gtggccggcg cacacctcgt ccggaagcga accgatccac
     4801 cgtctggccg tcatcagctc cccgcgcttc aacagccact cgccgtatcg gtcgatcaat
     4861 tccgccgaag cggccgcgtc gccggaggcg acacggtggt tgatcgcctc aacggggttg
     4921 tggtgagcat cgaaccactc cgatgccttt cggtgaagga tgggaagctc cttcgcgtga
     4981 ctctcctgga gacgggccga gagcacgccc gcaaacagat ggtgcagtcg gaaccagcgg
     5041 ccttcttcgt ccatggggct cacgaacagg ttctgccgat cgatcgcggc aagcacatcg
     5101 cgggaatccg accgcccggt caccgcgtca cacaagtcgg ccgacaggcg ctccaggatc
     5161 gaacagcgtt ccaggaaatc cagagtcgag gggtccaacc gggcaatcac ttcctcgacg
     5221 aggtagtccc gcacgtagcg ctggccgccg ctcagctccc cgatgaggcg ggacggatcg
     5281 gcggagccct tcagcgagga ggccaggatg tgcagccccg cgatccatcc ttccgtggta
     5341 tgcgaaatgg ccagcgcctc ttcgctggtc agggaaacgc cacccgcctc cgtgaggaag
     5401 gctctgatct cttcaatagt aaaggcaaga tcggactcgc ggatctccac cagctctccc
     5461 tgtccccgga gcttggccag tcggagcgcc gggtcaatcc gcgtgatgag cacaaggtga
     5521 gccgtgggcg gcagattctc gagcagcgac atcacgaggt cgttcacttc ggcgctctcg
     5581 gcgaggtggt agtcgtcgaa gatcagcatc gtgtcctgct gtcgctcggc aagaccgttg
     5641 aggaacgcaa cccagtgatt cggtcccggc gggctttccc gttgctcgag ccggtcgaga
     5701 gctgccccga ggtaggtggt cacccgctgc gcggagccgt ccagttgacc gaggtttacc
     5761 cagcagtggt cccgggaccg ttcgccgagc caggaggaga ccagggtggt cttgccgtac
     5821 cctgccggcg cggaaatcac cgtgagcttg cgcgcggcgc cgctctccag acgctcatag
     5881 aggcgcttgc ggtggacaac acttctgacg gggggtcgaa cgatctttgt ctggaggatc
     5941 gattcgcttc caaatccgct gtccgtcaaa gcggttgttc ctccgccgtt tgtactgggg
     6001 cgatagtact ccgagcgccg tcggcgggca agggcaagtc cagagcagga aagggcgcac
     6061 cctcccccgg ggcgctcgcc cctaggctgc catctccctc gaccggccct ctactggact
     6121 ttcggccggt gccaaccgcc attggccgcc ctattctgta gccgtcagga ttgatggagg
     6181 aggtgtccgg tgcggaggct ggcagagatg aattgcggta gaacgctgag acggttggtg
     6241 gcatcggcag cgcttctggt tgcgagcacg ggcgtggcac tttcccaaca ggccgcggcg
     6301 ggctcccagg aggtcgtcac gctggatcag gccatttccg cggcccgcgt gtccgccccc
     6361 gggctgaggc tggccagcgt t
//
LOCUS       DASRZS010000332         4984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_182470, whole genome shotgun sequence.
ACCESSION   DASRZS010000332 DASRZS010000000
VERSION     DASRZS010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182470"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFH83_08085"
     CDS             <1..423
                     /locus_tag="VFH83_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486364.1"
                     /translation="VLAASLVLVSLGGCAPSKYKPTANEEIYGTWTNPGAAHQKSVFS
                     ANGTWQQFTYAEDTAPSASGTFEIARKWKDPDGTIWYHENFERTTGVYQFKGQQLVRI
                     DKSGKQIETMYVEVPKFDPANYPKKLDPKSESYGIAYR"
     gene            498..962
                     /locus_tag="VFH83_08090"
     CDS             498..962
                     /locus_tag="VFH83_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486365.1"
                     /translation="MSGRRTFACLVALAVMVAVSGLAQDKYVPRADEECYGTWINDKT
                     INQGHIQKKICTADGNKDYLQVSDATPWFEDDQQIDAKWTDSDGNIWYKTFGAVRAGA
                     GIGTKWQELDKLSQSATVWEYEFAIVKDFSPDGYPSKVDSDDLRYRIFYRAK"
     gene            1168..1647
                     /locus_tag="VFH83_08095"
     CDS             1168..1647
                     /locus_tag="VFH83_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486366.1"
                     /translation="MKTGKVLLISSVFVVVVSLAGIAQDRYVAKADEEIYGTWVSENL
                     SKTQLQKYVLGAGIMKRYRLLSDSTPLDEATFEIDARWTDSDGNVWYRTHGVQTTGIY
                     KDYSYVELDKVNKAGTIWEAQFFSLGYKVEIDPAYAPKAFDPNSSYHGFQYRYRPEE"
     gene            complement(1770..2495)
                     /locus_tag="VFH83_08100"
     CDS             complement(1770..2495)
                     /locus_tag="VFH83_08100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HET6486367.1"
                     /translation="MYEVSVGDQVLRYEIIRSARARNMGVRIFPSGVRVVLPRGVPEQ
                     EAIAFMERVKGRVFRARQRVLGQDERLKSLTDIRCVDGARITVLGKPVRLRVLREDRT
                     RSRLTFDGVLTVRVDQSLAPAACEHQVKRKVEAWIKELVSAQVQRIVPGFGKQIGVLP
                     KGIRVKSQRKQWGSCGSNRVINLNWRLGLFPPQVLRYVVAHEMCHLRQMNHSKAFWQL
                     VASLVPDYEVQRQWLKFRGGGEG"
     gene            complement(2645..3805)
                     /locus_tag="VFH83_08105"
     CDS             complement(2645..3805)
                     /locus_tag="VFH83_08105"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HET6486368.1"
                     /translation="MPSTVYYGSPRQARLDAKETLPAKLDLILERLKLRERVKDETVV
                     IKMHTGTNIGYSTVHPVFVRKVVKAIKDGGGRPYVADVTWDAKGAEERGYSSETLGCP
                     VYAAAGPDDKYFYEHERPFKNLTKWKVAGLIQDATFLVNLAHAKGHPSCGYGGVFKNL
                     ALGCMAGSTRSAMHDAMQYDRYWWADKCDDAGRARIVAACPHSALVEDKNNPKELHLH
                     PEQCNQCGRCLQVAPAGSLKIDKVNFHAFQEALAISTSLSLGTFAPGKAVHLIIANQM
                     TPVCDCFGFTGMPVLPDAGIFGSDDIVACEQAVLDATAKLRLIEENLPTSMEVHTREG
                     HPLRWLHGPYKDPYLVAQYGEKLGLGTREYVIEDVLPVEKIERAPITYISAR"
     gene            complement(3933..4784)
                     /locus_tag="VFH83_08110"
     CDS             complement(3933..4784)
                     /locus_tag="VFH83_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET6486369.1"
                     /translation="MPASEGALFVEERKMKILEFIGEHRKATVVELCGRFKVSSATIR
                     NDLRDLETAGLLIRTHGGAMVKTKTGLEPDMSLRKVQNLEEKRRIAETALGLVEDGDT
                     IILDTGTTTYELARLLGQKRDLTVVTNDLPIAMLLEGFESVRVVVVGGMIRRKFHCTV
                     ASHFSGMNALSDLTVDKAFMACNGFSLEKGASTPDMAHSETKRLMISIAARVILLFDS
                     SKMGRNSFAIFAPLDRIDAIVTGSLRDEDRRSLEENGVETMTAAAGPAPADGPAGSAS
                     AGHAPGP"
     gene            complement(4823..>4984)
                     /locus_tag="VFH83_08115"
     CDS             complement(4823..>4984)
                     /locus_tag="VFH83_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012066688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET6486370.1"
                     /translation="WRQERDRSLRVEDGWTYFLVGWLSVIAEVFGKTIPLKGPFFDGL
                     RRIAEAERR"
BASE COUNT          959 a         1557 c         1555 g          913 t
ORIGIN      
        1 gttttggcgg catcccttgt gttggtttcg cttggaggct gtgctccttc aaagtacaaa
       61 ccgaccgcga acgaagaaat ctatggcacg tggacgaatc ccggcgcggc tcatcagaag
      121 agcgtgttct ctgcgaatgg aacgtggcaa caattcacct acgcggaaga cacggcgccc
      181 tcagcaagcg gcaccttcga aatcgccagg aagtggaaag atcccgatgg gaccatctgg
      241 taccatgaga atttcgagcg cacgacaggg gtctaccagt tcaaaggaca gcagttggtc
      301 cggatagaca agtcggggaa acagatagag accatgtacg ttgaagtgcc gaagttcgat
      361 cccgcgaact acccgaagaa gttggacccg aagagtgaga gctacggcat cgcctaccgc
      421 tgaacacaga tgttttcgtg tccacaagcg gcaggctgca aaacagtgca tgagcaagag
      481 aggcccacgg aggaagtatg agtggtagaa ggacgtttgc atgcctcgta gctcttgctg
      541 tgatggtagc tgtctcgggg ttggcgcagg acaagtacgt cccaagggca gacgaagagt
      601 gttatggaac gtggatcaac gacaagacca ttaaccaggg gcacattcag aagaagatct
      661 gcaccgcgga cggaaacaag gactacctcc aagtctccga cgcaaccccg tggtttgagg
      721 acgatcaaca aatagatgcg aagtggactg attcggacgg caacatctgg tacaagacct
      781 tcggagctgt aagagctggt gccggtatcg gcacgaaatg gcaggagctg gacaagttga
      841 gtcaatccgc aactgtctgg gaatacgagt tcgccatcgt gaaagacttc agccccgatg
      901 gctatccgag caaagtggat tcggacgacc tgaggtatcg catcttctat cgggcaaagt
      961 agtgaatctt cgacgtcgca aagccgctgc gggagaactt gcccgatcac gagagaacgt
     1021 gaaggaagaa gtatgaaggt cgcaaaggag caaggacggg tcggttttcc gaatccacgt
     1081 gggtgtggac aggcaggaag gagtctgatc caaagctgag tacctcatct atcatcgcac
     1141 ggcgcaagag gaagaaggcg ggagatcatg aagacgggaa aagtgcttct catttcatcg
     1201 gtctttgtgg tggtcgtttc actggcggga atagcgcagg acaggtacgt cgcaaaggcg
     1261 gacgaagaaa tctatgggac ctgggtcagc gagaatctat caaagacgca gttgcagaaa
     1321 tacgtccttg gcgctggaat catgaagcga taccggcttc tgtccgattc tacacccctc
     1381 gacgaggcta ccttcgagat cgacgccagg tggaccgact cagacggcaa cgtctggtac
     1441 aggacgcatg gcgttcagac cacgggaatc tacaaggact acagctacgt cgaacttgac
     1501 aaggtaaaca aggcaggcac gatttgggag gcacagttct tctctctcgg gtacaaggta
     1561 gagatcgacc ctgcatacgc tccgaaagcc ttcgatccaa acagcagtta ccacgggttt
     1621 caatatcgct accgcccaga ggagtaggac gccggcgggc tgactcgagg gtggcttctg
     1681 agcgtgtgga gtcatcgggc cacacgcgcg gcggttctcg gcctggtttg agaggcgtca
     1741 aagccctgcc ccggggacgc gtcgccgacc tatccttccc cgccgccgcg gaacttgagc
     1801 cactgccgct gcacctcgta gtcgggcacg agggacgcca cgagctgcca gaaggccttc
     1861 gagtggttca tctgcctcaa gtggcacatc tcgtgcgcga ccacgtaccg cagcacctgc
     1921 ggcgggaaca ggcccagccg ccagttcagg ttgatcaccc ggttgctccc gcagcttccc
     1981 cactgcttgc gctgcgactt cacccgaatg ccctttggaa gaacgccgat ctgcttgccg
     2041 aagcccggga cgatcctctg gacctgcgcg gagaccagct ccttgatcca ggcctcgacc
     2101 ttccgcttga cctggtgctc acacgccgcc ggcgcaagcg actggtctac ccggaccgtc
     2161 agcacgccgt cgaacgtcag gcgggaacgg gtacggtcct cgcgaagcac ccgcagccgg
     2221 accggctttc ccaggacggt gatcctcgcg ccatccacgc agcggatgtc cgtgaggctc
     2281 ttcagccgct cgtcctggcc caggacccgc tggcgtgcgc gaaacaccct tcccttgacg
     2341 cgctccatga acgcgatggc ctcctgctcc ggcactcccc ggggcagaac gacgcggacg
     2401 ccggaaggga agatccttac ccccatgttc cttgcccggg cactccgaat gatctcgtag
     2461 cggaggacct ggtccccgac gctgacctcg tacaccacgc tgctcctcga cgagcccgag
     2521 tgtagcgggc cgccgcggct ccgtcaccgt cagcggaggg ccgccgcggc tccgtcaccg
     2581 ccggtggatg gctccgcgga ggccggctcc cgcggaggcc agccccgcgg ggcgctgaga
     2641 ggtcctatct cgccgagata taggtgatcg gcgcgcgctc gatcttctcc acgggcagca
     2701 cgtcctctat cacgtactcc cgcgttccca ggccgagctt ctccccgtac tgcgccacga
     2761 ggtaggggtc cttgtacggg ccgtgcagcc agcgcagggg atggccctcg cgggtgtgca
     2821 cctccatgga cgtcgggagg ttctcctcga tcaggcggag cttcgcggtg gcatccagca
     2881 cggcctgctc gcaggccacg atgtcgtccg agccgaagat gccggcatcc ggcagcaccg
     2941 gcatgccggt gaagccgaag cagtcgcaca cgggggtcat ctggttggcg atgatgaggt
     3001 gcacggcctt gcccggggcg aacgtcccca gggaaaggct ggtgctgata gccaacgcct
     3061 cctggaacgc gtggaagttc accttgtcga tcttcaggct cccggccggt gccacctgca
     3121 ggcaccggcc gcactggttg cactgctcgg gatgcaggtg caactccttc gggttgttct
     3181 tgtcctccac cagggcggag tgagggcagg ccgcgacgat ccgtgcccgc ccggcgtcgt
     3241 cgcacttgtc cgcccaccag tagcggtcgt actgcatggc atcatgcatc gcgctccgcg
     3301 tggaaccggc catgcaaccc agcgcgaggt tcttgaacac cccgccgtag ccgcagctcg
     3361 ggtggccctt ggcgtgggcc aggttcacca ggaacgtcgc gtcctggatg agccccgcca
     3421 ccttccactt cgtgaggttc ttgaatggcc gctcgtgctc gtagaaatac ttgtcgtcgg
     3481 gaccggccgc ggcgtacacg gggcatccca gcgtttcaga agagtagccg cgttcctcgg
     3541 cgcccttggc gtcccacgtg acatccgcca cgtacgggcg gccgccgcca tccttgatgg
     3601 ccttcaccac cttgcgcacg aacaccgggt gcaccgtgga gtacccgatg ttggtgcccg
     3661 tgtgcatctt gatgaccacg gtctcgtcct tgacgcgctc tcgcagcttg aggcgctcca
     3721 ggatcaggtc gagcttggcc ggaagcgtct ccttggcatc caggcgggcc tggcgcgggg
     3781 agccgtagta cacggtgctg ggcatgggac acctctcctg ctgaagccgg cggcgcgagg
     3841 ggccgcgggg ctccctggcc tattgaatcc gtccggggga taccggacag ggaatcccaa
     3901 ttccgcggga ggttcaagtc ctcccggtcc gctcagggac cgggcgcgtg accagcgctg
     3961 gcggaccccg ccggcccgtc tgccggggcc ggaccggccg ctgccgtcat cgtctccacg
     4021 ccgttttctt ccagggagcg ccggtcctcg tcgcgcaggg agccggtcac gatggcgtct
     4081 atgcggtcca gcggcgcgaa gatggcaaag gagtttcgcc ccatcttgct ggagtcgaac
     4141 agcaggatca cccgtgcggc gatggagatc atgaggcgct tggtctccga gtgcgccata
     4201 tccggagtgc tggcgccctt ttccagcgag aagccgttgc aggccatgaa ggccttgtcc
     4261 acggtgaggt cggagagagc gttcatgccc gagaagtggg acgccacggt gcagtggaac
     4321 ttgcggcgga tcatcccgcc gaccaccacc acgcgcacgg actcaaaacc ctccagcagc
     4381 atggcgatgg gaagatcgtt ggtcaccacc gtgaggtccc gcttctgccc caggaggcgc
     4441 gccagctcgt aagtggtggt ccccgtgtcc aggatgatgg tgtccccgtc ttccacgagg
     4501 cccagggctg tctccgcgat gcgccgcttc tcttccaggt tctggacctt gcgaaggctc
     4561 atgtccggct ccaggccggt cttggtcttc accatggccc cgccgtgggt gcggatgagc
     4621 aggcccgcgg tctccaggtc ccgcaggtcg ttgcggatgg tggcgctgga caccttgaac
     4681 cgtccgcaca gctccaccac ggtggccttg cggtgctccc cgatgaactc gaggatcttc
     4741 atcttgcgct cttcgacgaa aagagctcct tccgatgccg gcatccgctg ccccttctct
     4801 cactcccgga tgatcggtta tctcatcttc tctcggcctc tgcaatcctt cgaaggccgt
     4861 cgaagaatgg gcctttcaga ggaattgttt tcccgaagac ctccgcaatg acgctgagcc
     4921 agcccacgag gaagtacgtc cacccgtctt ccacgcgcag gcttcgatcc cgctcctggc
     4981 gcca
//
LOCUS       DASRZS010000333         4671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4035154, whole genome shotgun sequence.
ACCESSION   DASRZS010000333 DASRZS010000000
VERSION     DASRZS010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4035154"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VFH83_08120"
     CDS             complement(<1..1036)
                     /locus_tag="VFH83_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HET6486371.1"
                     /translation="MAAPLTEAILRHGTVGLEAYEASGGYQGLRKAIAMEPAEVQDVV
                     KKSGLRGRGGAGFPTGQKWSFVPMGDSEAHYFVVNADEKEPGTMKDRFLMEGNPHQLV
                     EGAIIAARAVKAQTAYIFLRREYLKAAAGITRAIQEAYQKRYLGQGILGSSYSLEMRL
                     HLSVGRYMCGEETGLINALEGRRATPRNKPPYPQTSGLWGKPTVVNNAETVCNLPHIL
                     REGPDWYKALSASPDAGTKMFGVSGRVKRPGVWELPMGTPIREILYGHAGGMADGLEF
                     RALIPGGASTEFLGPEHLDLKMEFDSVVKAGSRLGTGTMVVLDNKTCPVGMVLNLERF
                     FARESCGWCTP"
     gene            complement(1017..1508)
                     /gene="nuoE"
                     /locus_tag="VFH83_08125"
     CDS             complement(1017..1508)
                     /gene="nuoE"
                     /locus_tag="VFH83_08125"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HET6486372.1"
                     /translation="MLSSHERERIEEERRHYEDPRAAVNEALMIVQESRGWVSDEGVG
                     DIAEILGMTRDEVDAIATYSDLVFRRPVGKHVILLCDSVSCFVTRYERIQEHLKQTLG
                     IEPGQTTADGQFTLLPVGCLGVCEQGPAMTIDGEVYGNLTPESTDAVIREYREGKHGS
                     ASH"
     gene            complement(1554..3296)
                     /gene="nuoC"
                     /locus_tag="VFH83_08130"
     CDS             complement(1554..3296)
                     /gene="nuoC"
                     /locus_tag="VFH83_08130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HET6486373.1"
                     /translation="MAETSGPIQELSTRFAEVVSQDSKDGIPTAWVPPHRCHDALAFL
                     KTPAGGPYETLLDLTAIDERSRTHREGQPPAAFTVVYHLVSYAGNRELRVKTALSGEA
                     PSLATVTDLWINANWYEREVWDMFGIRFDGHPNLRRILMPPWWDGHPLRKEHPARATE
                     MGLFQMTDDDMMRMLGQLEFKPEEWGMQRKSGEHELMFVNVGPQHPSAHGVLRAVAQL
                     DGEEIVDLVMDIGYHHRGAEKMGERQSWHTYIPYTDRIDYLGGVMNNFPYVLAVEKLA
                     GIVVPDRAKVIRIMMAELFRVISHLVYYGTFAQDIGQMSPVFYMFNDRERAFDIVEAI
                     TGGRMHPSWFRIGGVAQDLPEGWDTLVGQFITYLRHRLKDYDAAVLGNRILKVRSKGI
                     GIVSKEEAIQWGATGPMLRATGFEWDLRKKRPYGGYDQFDFDVPTGTGGDSFARVVVH
                     VEEMRQSLRIIEQCLKGMPAGPYKSDHPLATPPLKVRTTHDIETLIGHFLSVSWGPVI
                     PAGEASATIEASKGNQTYYLVSDGGTMSYRTRIRTPSFAHLQLIPMMSRGLTIADLVV
                     ILGSVDYVLADVDR"
     gene            complement(3313..3900)
                     /gene="nuoB"
                     /locus_tag="VFH83_08135"
     CDS             complement(3313..3900)
                     /gene="nuoB"
                     /locus_tag="VFH83_08135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HET6486374.1"
                     /translation="MPIERRSPFSDILLAKRTDLIAWGRKYSIWPFNFGLSCCYVEMA
                     TSLTSRYDIARFGAEVLRGTPREADLMIVAGTVFKKMAPLVRYMWQQMMEPRWVISMG
                     SCSNSGGMYDIYSVVQGVDSFMPVDVYVQGCPPSPDAFMRGLVMLADVVGSEQRPLSW
                     LVGPQGVVRPPMPSQRDRMRGTRSRQTELTSPDKV"
     gene            4134..>4671
                     /locus_tag="VFH83_08140"
     CDS             4134..>4671
                     /locus_tag="VFH83_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486375.1"
                     /translation="MTALASVPGCGQVPLSDYSDVLARRGQLGVPLAEARAVEARIGQ
                     LLVVNVDGFGYSGKLALEPAFVPMLESLQIGGVIPHYGSSSYDRIRATNDALAGMTDL
                     PLLLCADIVRLAVGSRSATFGDGYVGGFLGKYRKLSDPDLQTLAALNAFVFAALGINV
                     ALGPTVDDSTRDPRTAERA"
BASE COUNT          730 a         1634 c         1468 g          839 t
ORIGIN      
        1 agggcgtgca ccagccgcag gactcccggg caaagaagcg ctccaggttg agcaccatgc
       61 ccaccgggca ggtcttgttg tccagcacca ccatggtccc ggtgcccagc cggcttcccg
      121 ccttcaccac ggaatcgaac tccatcttga ggtcgaggtg ctccgggccc aggaactcgg
      181 tggacgcgcc gccggggatg agcgcccgga actccagccc gtccgccatt ccgccggcat
      241 ggccgtagag gatctcgcgg atgggggttc ccatgggaag ctcccacacg cccggcctct
      301 tcacccgccc gctgacgccg aacatcttgg tgccggcatc cggggaagcg ctgagcgcct
      361 tgtaccaatc cggtccctcg cgcaggatgt gagggaggtt gcagacggtc tcggcgttgt
      421 tgaccaccgt cggcttcccc cacagcccgc tggtctgggg atagggaggc ttgttgcggg
      481 gcgtggcgcg cctgccctcc agcgcattga tgagtccggt ttcttcgccg cacatgtagc
      541 ggcccacgct caagtggagc cgcatctcca gggaatagga ggagccgagg attccctgcc
      601 ccaggtagcg cttctgatag gcctcctgga tggcccgggt gatccccgcc gcggccttca
      661 ggtattcgcg gcgcagaaag atgtacgcgg tctgggcttt caccgcccgc gccgcgatga
      721 tcgcgccctc tacgagctgg tgcgggttcc cttccatgag gaaccggtcc ttcatggtcc
      781 ccggctcctt ctcgtccgcg ttgaccacga aatagtgcgc ctcgctgtcc cccatgggca
      841 cgaagctcca cttctgcccc gtggggaagc cggccccgcc gcgaccccga agtccggact
      901 tcttgaccac gtcctggact tccgccggct ccatggcgat ggcctttcgc aaaccctggt
      961 atccgccgga agcctcgtag gcctccaggc ccacggtgcc gtggcggagg atggcctcag
     1021 tgagaggcgc tgccatgctt tccctcgcga tactcgcgaa tcaccgcgtc cgtggactcc
     1081 ggggtgaggt tcccgtacac ctcgccgtcg atcgtcatgg ccggcccctg ctcgcatacg
     1141 ccaagacagc ccaccggcag cagcgtgaac tggccgtccg ccgtggtctg cccgggctcg
     1201 atccccaggg tctgcttgag gtgctcctgg atccgttcgt atcgggtcac gaagcagctc
     1261 acgctgtcgc acaggaggat cacgtgcttg cccacgggtc gacggaagac caggtcggag
     1321 tacgtggcga tggcgtccac ctcgtcccgg gtcatcccca ggatctccgc gatgtccccc
     1381 acgccctcgt cgctcaccca gccccggctc tcctgcacga tcatgagcgc ttcgttgacc
     1441 gcggcgcgcg gatcctcgta gtgccgccgc tcctcctcga tgcgctcgcg ctcatggctg
     1501 ctcagcacga cctctccttt ccgccgccca ggcccctgaa cctcggcgta tcatcagcgg
     1561 tccacgtccg ccagcacgta gtccacgctc cccaggatga ccaccaggtc cgcgatggtc
     1621 agaccgcggc tcatcatcgg aatgagctgc aggtgcgcga aggatggggt gcggatgcgc
     1681 gtgcggtagc tcatggtccc gccgtcgctc accaggtagt aggtctggtt tcccttgctt
     1741 gcctcgatgg tggcgctggc ctcccccgcc gggatcacgg ggccccagct cacgctcagg
     1801 aaatgcccga tgagcgtttc gatgtcgtgg gtggtccgca ccttcagcgg cggcgtggcc
     1861 agcggatggt ccgacttgta cggaccggcc ggcatcccct tcaggcactg ctcgatgatc
     1921 cgcaggctct ggcgcatctc ctccacgtgg acgaccaccc gggcaaagga gtccccgccg
     1981 gtccccgtgg gaacgtcgaa gtcgaactgg tcgtacccac cgtagggccg cttcttgcgg
     2041 agatcccact cgaacccggt ggcgcggagc atgggccccg tggcccccca ctggatggcc
     2101 tcttccttgc tgacgatccc gatccccttg gacctcacct tgaggatccg atttcccagc
     2161 acggccgcgt cgtagtcctt gagccgatgc cgcaggtacg tgatgaactg gccgaccagg
     2221 gtgtcccatc cttccgggag gtcctgggca accccgccga tacggaacca cgagggatgc
     2281 atgcggccgc cggttatggc ctccacgatg tcgaaggcgc gctcgcggtc gttgaacatg
     2341 tagaagacgg gggacatctg gccgatgtcc tgggcaaacg tcccgtagta gaccaggtga
     2401 ctgatcacgc ggaaaagctc cgccatcatg atccggatca ccttggcccg atccgggacc
     2461 acgatccccg ccagcttctc caccgccagc acgtagggga agttgttcat caccccgccc
     2521 aggtagtcga tgcggtccgt gtacgggatg tacgtgtgcc agctctggcg ctctcccatc
     2581 ttctccgcgc cgcggtggtg gtacccgatg tccatcacga ggtccacgat ctcctcaccg
     2641 tccagttggg ccacggcgcg gagcaccccg tgcgcgctgg gatgctgcgg gcccacgttc
     2701 acgaacatga gctcgtgctc cccgctcttg cgctgcatcc cccattcctc gggcttgaac
     2761 tcgagctgtc cgagcatccg catcatgtcg tcatcggtca tctggaacag ccccatctcg
     2821 gtcgcgcgag cggggtgctc cttgcgcagg ggatgcccgt cccaccaggg cggcatgagg
     2881 atgcggcgca ggttgggatg cccgtcgaag cggatgccga acatgtccca gacttcccgc
     2941 tcgtaccagt tcgcgttgat ccagaggtcc gtgacggtcg ccagagaggg agcctccccg
     3001 ctcagcgcgg tctttactcg cagctcgcgg ttgccggcgt aggaaaccag gtggtagacg
     3061 acggtgaacg ccgccggcgg ctggccctct cgatgggtac gggagcgctc atcgatggcc
     3121 gtaaggtcca ggagagtttc gtacgggccg ccggcgggcg tcttcaggaa cgccagggcg
     3181 tcgtggcaac gatgcggcgg cacccacgcc gtcgggatgc cgtccttcga gtcctgcgag
     3241 accacctccg cgaaccgggt ggaaagctcc tggatggggc cgctggtttc cgccatgcac
     3301 gacctctccg ccctaaacct tgtccggaga ggtcagctcg gtctgtctgc ttcgcgttcc
     3361 gcgcatccgg tcccgctggg acggcatggg cggcctcacc acgccttgag gtcccacgag
     3421 ccaggagagc ggccgctgct cgctacccac cacgtccgcc agcatcacca ggccgcgcat
     3481 gaacgcgtcc gggctgggcg gacatccctg gacgtagaca tccacgggca tgaaggagtc
     3541 caccccctgg accacgctgt agatgtcata catcccgccg gagttcgagc aggagcccat
     3601 ggagatgacc cagcgcggct ccatcatctg ctgccacatg tagcggacca ggggcgccat
     3661 cttcttgaag acggtccccg ccacgatcat gaggtccgcc tctcggggcg ttccccggag
     3721 gacctcagcg ccgaatcgcg caatgtcgta tcggctcgtc agagacgtgg ccatctccac
     3781 gtaacagcag gaaagcccga aattgaaggg ccagatcgaa tactttctgc cccaggcgat
     3841 gagatcggtg cgcttcgcga gcaggatgtc gctgaagggg gatctgcgct cgatcggcat
     3901 ggttccgccg gacctttcta gtgctcagac gtatgtggcc gctccgagga gcgatggaaa
     3961 tccgccgcgc ccgaaaacag ccagaccagg cccggcaggc cgaggcaaat atagttgcgc
     4021 cgtccggcga ccgtcaacaa agcccttgcg cgcccgcccc gtctctccta tcctcccata
     4081 ccgtgcatcc gttcgcgagc tgaaccgtgc cgaaaatcct tcttgtctgc ctggtgacgg
     4141 ctctagcctc tgttcccggt tgcgggcagg tcccgctctc cgactacagc gatgtgctgg
     4201 ctcggcgggg tcaactcggc gtgcccctgg cggaagcgcg cgccgtcgag gcgcggatcg
     4261 gccagctcct cgtggtgaac gtggacggct tcggctactc ggggaagctg gccctggagc
     4321 ccgccttcgt cccgatgctg gagagcctgc agatcggcgg cgtgatcccg cactacgggt
     4381 cgagcagcta cgatcgcatc cgcgccacca acgatgccct ggccggcatg accgatctcc
     4441 cgctcctgct ctgcgccgac attgtccgcc tggccgtggg ctcgcggtcc gccacgttcg
     4501 gggacggcta cgtgggtgga ttcctgggca agtaccggaa gctttccgat ccggacctcc
     4561 agacgctcgc cgcgctcaac gcgttcgtct tcgccgccct cgggatcaac gtggcgctgg
     4621 gtccgacggt ggacgacagc acccgggatc cgcgcaccgc ggaacgggcg c
//
LOCUS       DASRZS010000334         2346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1343369, whole genome shotgun sequence.
ACCESSION   DASRZS010000334 DASRZS010000000
VERSION     DASRZS010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343369"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="VFH83_08145"
     CDS             <1..161
                     /locus_tag="VFH83_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET6486376.1"
                     /translation="FIRVRPARSARQGRNPLTGETIMIAAKPATRVPRFTFSKTFKEA
                     ALKAKVKK"
     gene            complement(210..584)
                     /gene="mutT"
                     /locus_tag="VFH83_08150"
     CDS             complement(210..584)
                     /gene="mutT"
                     /locus_tag="VFH83_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015400595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HET6486377.1"
                     /translation="MRVTAAVIEEKGKVLLARRGPGRHLAGKWEFPGGKIEPGETPEQ
                     SLERELEEELAIRTRVGELLCSTSFEHDGISLELLVYRVERTAGEPQLREHLELAWVP
                     PREVASYDLADSDRTVARALYG"
     gene            complement(581..1156)
                     /gene="nth"
                     /locus_tag="VFH83_08155"
     CDS             complement(581..1156)
                     /gene="nth"
                     /locus_tag="VFH83_08155"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET6486378.1"
                     /translation="MARHTSDPFRVLVATMISLRTKDEVTSVAADRLLAEAPGPSALE
                     ALPEPRIEKLIYPAGFYHTKARNLRKMAALLRERHQGAVPSEMEDLLALPGVGRKTAN
                     LVRNLGFNLPGICVDTHVHRISNRFGWVTTRGPAETERALMAVLPRRYWIVINELLVR
                     YGQSVCTPLSPRCSACPVSAWCARVGVGKSR"
     gene            complement(1306..1833)
                     /locus_tag="VFH83_08160"
     CDS             complement(1306..1833)
                     /locus_tag="VFH83_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET6486379.1"
                     /translation="MKSPRKSTRPVVTKYKVAQEIVYPLQGVGHIQTIEERPFQDKKL
                     LYYIIYLEVSDMTVMVPVDKADQLGIRAIVGKKESQKALKLIAEEFEPIPADWKLRYQ
                     MNLDLLKKGSILDIANVVRALYHRSRIKELPILERKLYDSALKLLIDEISFSLGKSKD
                     EVEQLVFARLKLNAK"
     gene            1891..>2346
                     /locus_tag="VFH83_08165"
     CDS             1891..>2346
                     /locus_tag="VFH83_08165"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET6486380.1"
                     /translation="MNRRRIAALIAFPLLVAALVVPILLWRQQIWGLFASTQRLRQWF
                     AGWGIWAPVVFLGIQALQVVVFAIPGEFVQIAGGFLFGGWVGTLLSVAGILIGSTVAF
                     FLARLLGRPFVAALFSKDQVERVERLLASRSARTIFFLLFLIPGIPKDIL"
BASE COUNT          358 a          792 c          757 g          439 t
ORIGIN      
        1 tgttcatccg cgtccgaccg gcccgctccg ctcggcaggg gaggaatccc ttgaccgggg
       61 agacgatcat gatcgccgca aagcccgcca cccgggttcc gaggttcacg ttcagcaaga
      121 ccttcaagga agcggccctg aaggcgaagg tgaagaagta acaggctttc cgcgggtcac
      181 ggccatgagg aggcgggcgg cagcccgcct cagccgtaca gcgcccgcgc cacggtccga
      241 tccgaatccg ccagatcgta ggaagcgacc tcccggggcg gcacccaggc cagctccagg
      301 tgctctcgta gctgcggctc gccggcggtc cgttccaccc gatacaccag aagctccagc
      361 gatatcccgt cgtgctcgaa gctcgtgctg cacaggagct cgcccacgcg ggtacggatc
      421 gccagctcct cttcgagctc ccgctccagc gactgctccg gggtctctcc gggctcgatc
      481 ttgccgccgg gaaactccca cttgcccgcc aggtgtcttc ccggtccccg acgagccagc
      541 agcaccttgc ccttctcctc gatgacggcg gccgttaccc tcatcgcgac ttccccacgc
      601 ccaccctcgc gcaccacgcg ctcaccgggc acgcgctgca ccgcggggag agcggcgtgc
      661 agaccgattg gccatagcgg acgagaagct cgttgatgac gatccagtaa cgccgcggga
      721 gcaccgccat gagcgcccgc tcggtctccg cgggaccgcg cgtggtgacc catccgaacc
      781 ggttggagat ccggtgcacg tgggtatcca cgcagatgcc gggcaggttg aagcccaggt
      841 ttcggaccag gttggcggtc ttccgtccca ccccgggaag cgccaggagg tcctccatct
      901 cggaagggac cgctccctga tggcgttcgc ggagcagcgc ggccatcttc ctcaggtttc
      961 gcgccttggt gtggtagaag cccgcgggat agatcagctt ctcgatgcgg ggctccggca
     1021 aggcttcgag ggccgatggc ccgggagcct ccgccagcag acggtcggcg gccacgctgg
     1081 tgacctcgtc cttggtccgc aaggagatca tggtggcaac gaggacccga aacggatccg
     1141 aggtgtgtcg cgccacggcg gagacggagg gaaggggcgc ctgggctccc tcgagccggc
     1201 gcgcgatggc gcccaggatg cgcgcccagg ggactcgaac cgtcgttcgg cggggccgcc
     1261 ggacgggacc cgggtggtgg gcccgcgcgg tccccgaacg tcccctcact tcgcgttcaa
     1321 cttgaggcgg gcaaagacga gctgttccac ctcgtccttg ctcttcccca gggagaagga
     1381 gatctcgtcg atgagcagct tgagggcgct gtcgtagagc ttgcgctcca ggatgggcag
     1441 ctccttgatc cggcttcgat ggtacagggc gcgcaccacg ttggcgatgt ccaggatgct
     1501 ccccttcttg agcaggtcca ggttcatctg gtagcgcagc ttccagtcgg ccggtatggg
     1561 ctcgaactcc tccgcgatca gcttgagcgc cttctgcgac tccttcttgc ctacgatggc
     1621 gcggatcccc aactggtcgg ccttgtccac cgggaccatc accgtcatgt ccgagacctc
     1681 gaggtagatg atgtagtaca gaagcttctt gtcctggaag ggtcgctcct cgatggtctg
     1741 gatgtggccg acgccctgga ggggatatac gatctcttgg gcgaccttgt acttggtcac
     1801 cacggggcgc gtcgacttgc ggggggattt catatcgccc attgtaccga agattcccaa
     1861 ctgggtcaaa ccaggggtat actggcgccg atgaaccgac gtcgaatcgc ggccctgatc
     1921 gccttcccgt tgctcgtcgc ggccctggtc gttcccatcc ttctctggcg acagcagatc
     1981 tgggggttgt tcgcctccac ccaacggctt cgacagtggt tcgccgggtg gggcatctgg
     2041 gcgcccgtcg tgttcctggg catccaggcc ctccaggtcg tcgtgttcgc catcccgggg
     2101 gagttcgttc agatcgccgg aggctttctc ttcggcggat gggtgggcac ccttctctcc
     2161 gtggcgggca tcctgatcgg ctccaccgtc gccttcttcc tggccaggct cctgggacgg
     2221 ccgtttgtgg cggccctctt ctccaaggat caggtggaac gggtcgagcg cctgctggct
     2281 tcccggagcg cacggaccat cttcttcctg ctgttcctca ttccgggcat ccccaaggac
     2341 atcctg
//
LOCUS       DASRZS010000335         6521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3651007, whole genome shotgun sequence.
ACCESSION   DASRZS010000335 DASRZS010000000
VERSION     DASRZS010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3651007"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="VFH83_08170"
     CDS             <1..1590
                     /locus_tag="VFH83_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA activase"
                     /protein_id="HET6486381.1"
                     /translation="QPLLDRVHWVHAADILETACVVADTPGLYPVYLTSFLCSPDSMT
                     IEYFKRILDERGKPYLILQLDDHDSTLGYETRVEAGVASFRNHMGRDAAKAAAAAAAH
                     AGTQTGGAGEAGTQTAAGVLSVIPRTSARLDGRTLLFPVWDPLVNPLLAANLRREGID
                     ARVLSEDPLVIRKSMRHNTGQCLPLNVIVQEAMDYVRGHGLVPSATSLWIPHSLLSCN
                     FGMFPSYMKSVFEAEGMGGLQVYRGDMYYLDISLRATLNAYRAFLVGGLLRRVGCRLR
                     PYEKVPGATDEAIRRAMQTLIPAFEGQGSKVQAYREAAAWFATVSVADAPRRPKVAVF
                     GDIYVRDNDVMNQGLVHAIEAAGGEVLTTPYTEYVRIVAHAYFQKLLESGDRLKSLGY
                     RALWKLVDTVGRDCRAPFEPYLDPEPAMDAAHNQELVQAFGLRTEHAGESFDNLLKIA
                     HLASAYPDLELFVQASPSFCCPSLVTEAMARDIEKRTGVPVVSITYDGTGQYRNDSVV
                     PFLKLARERGRASVPSASRAG"
     gene            complement(1606..3018)
                     /gene="murD"
                     /locus_tag="VFH83_08175"
     CDS             complement(1606..3018)
                     /gene="murD"
                     /locus_tag="VFH83_08175"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET6486382.1"
                     /translation="MRIDPGSIPGMRVTVMGLGVNGGGLASAQFFARRGATLTVTDLR
                     DEKALAESISRLASFPVRYVLGRHEESDFVNADVVIKNPGVATSSPYLTAAREHDVAI
                     ETDLSVFLSIARNPIIGVTGSKGKSTTASAIAFGLSRIDPRSRLGGNITVSPLSFVDD
                     LAGDAPVVLELSSWQLGDLRGRGLLAPVVSAFTIILRDHMDKYSGMGDYIADKMVLVQ
                     GQTRNQKAVFNLDDPWQRPFPRQTASRTYYYSREELPDGLAGGWLEPGRGMGMTVAGE
                     TPRQILGSALIPGEHNRMNLLCAGLCLQLYGVKAEVIRKALSEFPGVEHRLEMFVESD
                     GVRYYNDSAATIPDATAEGLKSLPEPVVLILGGTDKNLDFSPLRDVVKLPSAVVLLEG
                     SATRKVLPLLEEQGVPYEGPFDTLDRAIDVAMGKARDAVGPRGSSVLFSPGCTSFGMF
                     ANEFDRGRKFKEAVKARLAR"
     gene            complement(3008..3952)
                     /locus_tag="VFH83_08180"
     CDS             complement(3008..3952)
                     /locus_tag="VFH83_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486383.1"
                     /translation="MEQTSNAIRVGASDRDLNALLEGIREGERPFTRHFSQGEDFFLL
                     LEREFEVPQFPIHHDVRDPKPPARYMEKLGSVVEQVVALAPQVLRELIYYFDPAEILR
                     PCFYRLFRVDDCVYLFLARVDLTMRPATSTVIDRGTNDVTPHYRTRRLFVENCVVPLA
                     AVTREDGRITSFQVRQTISQTWIGEQGRGYFVQGIWMDADLTKFFSRLFLPQGKRTYP
                     YYPYLCKYKTICHSVLSLAPEGRKTTIPWLHRVIQFLLPAMDRIQTVMRTATFSEDIG
                     YYRELKARVPEQWYEPWKNIRVEAYFNEQETKEYRIED"
     gene            complement(4045..6024)
                     /locus_tag="VFH83_08185"
     CDS             complement(4045..6024)
                     /locus_tag="VFH83_08185"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HET6486384.1"
                     /translation="MLKLRSGVDILVEVGGLRTVSEATRFLDRSLDSRNRAKLSRITN
                     PDALLRIANSIAMCAPDGVLVVTASQEDVDACRQISLDTGEECPLAMKDHTLHFDLPE
                     DQGRMVDQTFYIANEDEEVSVLAKKMLRAEALDYIRATMKGIMKGKTMLIGFYSRGPI
                     GAQGAVPGLMISSSAYVLHSANILYRCVYDQFDAEAKRAGVFFTNVHSQGTNRSEDIG
                     KARIFMDRSWFTTFSMYCTYAGNTLMLKKGNHRFAVDLCTYYRRGSELSEHMFITGMR
                     GPGGRVTFFAGAAPSGCGKTTTAMVGTDYVGDDLSQIWIAEDGTLRAVNPEKGIFGIV
                     EDLNREGDPYLYKCLREPGTEVIWSNVLVDFDSVPHWTGSGETPPVKGRNFQGDWWPG
                     KTDKNGKPVPVSNPNARITLSNLAIANYNATMADDPRGVPIRVITYSGRDSDTMPPVW
                     VAKNPDHGVVIGASILSAATATEVGAKGVNRQPWANSPFIPCPLGDYMEAQFEFFNSP
                     KFSPEGRPLIAGLNYFLLDCARGGDLVKLLGEKRDVRAWLSWLERYAHGEVQAIETPI
                     GMIPRYEDLQTIFRSLVTKDYARSLYDKQFSFYVDNILGRIQLQEDAYRKEKRIPARL
                     FEIYGEQKKGLLALKERFGPVVSPDQMAEAATRAS"
     gene            complement(6124..>6521)
                     /locus_tag="VFH83_08190"
     CDS             complement(6124..>6521)
                     /locus_tag="VFH83_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6486385.1"
                     /translation="DHFVEKYAVAHGRDVRRISNSAIDMLMAYHWPGNVRELENCVER
                     AVLLSDDNVIHGHHLPPTLQTAEASGTVPSGTLKATLERVEQELLLEALKSSRGNMSS
                     AAASLGITERIMGLRVRKYRIHARRFRGP"
BASE COUNT         1097 a         2125 c         2131 g         1168 t
ORIGIN      
        1 cagcccctcc tggatcgggt gcactgggtt catgcggccg acatcctgga gactgcctgc
       61 gtggttgcgg atactccggg gctgtatccg gtgtacctca cctcgttcct gtgctcgccg
      121 gacagcatga cgatcgagta cttcaagcgc atcctggacg agcgcggcaa gccgtacctg
      181 atcctgcagc tcgacgacca cgactccacg ctcggttacg agacgcgggt cgaggccgga
      241 gtcgcctcct tccgcaacca catgggtcgg gacgccgcga aggcggccgc ggccgccgcg
      301 gcgcatgccg gcacgcagac cggcggcgca ggggaggccg gcacccagac ggccgccggt
      361 gtcctgtcgg tgatccctcg gacgtccgca agactcgacg gtcggacgct gctcttcccg
      421 gtctgggacc ccctggtgaa tcccctcctg gccgcaaacc tgcgtcggga gggcattgac
      481 gcccgggtac tctcggagga tcccctggtg atccgcaagt ccatgcgcca caacaccggg
      541 cagtgcctgc cgctgaacgt catcgtccag gaggcgatgg actacgtgcg tggccatggg
      601 cttgtcccgt cggctacttc gctctggatc ccccactcgc tcctttcctg caacttcggg
      661 atgttcccgt cctacatgaa gagcgtcttc gaagccgagg ggatgggcgg cctgcaggta
      721 taccggggcg acatgtacta cctggatatc tccctacgcg ccacgctgaa cgcgtaccga
      781 gccttcctgg tcggcgggct tctgagaagg gtagggtgcc gactacgtcc ctacgagaag
      841 gtccccggcg ccacggacga ggcgattcgc cgcgccatgc agaccctgat cccagcattc
      901 gaaggccagg gctccaaggt ccaggcgtat cgggaggccg ccgcgtggtt cgcaacggtg
      961 tcggtcgccg acgcgccgcg caggcccaag gtcgccgtgt tcggtgacat ctacgtccgg
     1021 gacaacgatg tgatgaacca ggggctcgtt cacgccatcg aggcagccgg cggagaggtc
     1081 ctgacgaccc cgtacacgga gtacgtgcgg atcgtggccc acgcgtattt ccagaagctt
     1141 ctcgaaagcg gagaccgtct caagtccctg ggataccggg cgctctggaa gctcgtggat
     1201 accgtcggaa gggactgccg ggcccccttc gagccatacc tggaccccga accggccatg
     1261 gacgcggcgc acaaccagga actggtccag gcgttcggtc tgcgcacgga gcatgccgga
     1321 gagtccttcg acaacctgct gaagatcgcc cacctggcaa gcgcatatcc ggatctcgag
     1381 ctcttcgtgc aggccagccc ctcgttctgc tgtccgtcgc tggtgaccga agccatggct
     1441 cgggacatcg agaagcggac cggggtcccg gtggtgagca ttacctacga tggcaccggt
     1501 cagtatcgca acgattccgt cgtgccattc ctgaagctgg ctcgggagcg cggccgggct
     1561 tcggtgccga gcgcctcccg cgcgggatag gcggtctctc gcgcgctatc gggcaagccg
     1621 cgccttgacg gcttccttga acttcctgcc gcggtcgaac tcgttcgcga acatcccgaa
     1681 ggacgtgcac cctggagaga aaaggacgga cgaccctctg ggtcctaccg cgtcccgagc
     1741 cttccccatc gccacgtcaa tggcccggtc aagcgtatcg aagggccctt cgtagggcac
     1801 accctgctcc tccagaaggg ggaggacctt gcgcgtggcg ctcccttcca ggaggacgac
     1861 cgcggaaggc agcttcacaa cgtcccgcag gggggagaag tccaggttct tgtcggtgcc
     1921 ccccaggatc aggacgaccg gctccggaag ggacttcagc ccctcggccg tagcgtccgg
     1981 gatcgtagcg gcggaatcgt tgtagtagcg gacgccgtcg gattccacga acatctccag
     2041 ccgatgctct acgcccggaa actccgaaag tgccttgcgg atcacctccg ccttgacgcc
     2101 gtagagctgg aggcacagcc ccgcgcagag aaggttcatc cggttgtgct ctccggggat
     2161 cagcgccgag ccgaggatct gcctcggcgt ctccccagcg accgtcatcc ccatcccgcg
     2221 gccgggctcg agccagccgc cggccaaccc gtcgggaagc tcctcccgcg agtagtagta
     2281 ggtccgcgag gcggtctgtc gagggaacgg ccgctgccac ggatcatcca ggttgaacac
     2341 cgccttctgg ttccgagtct gtccctggac caggaccatc ttgtccgcga tgtagtctcc
     2401 catgccggag tatttgtcca tgtggtcccg gaggatgatg gtgaacgccg aaaccacggg
     2461 cgccagcagg cccctgccgc gcaagtctcc gagctgccac gaggagagct ccagcaccac
     2521 cggcgcgtcg ccggcaaggt cgtccacgaa cgaaagggga gatacggtga tgttcccgcc
     2581 gagccgcgaa cgcggatcga tacgggagag cccgaaggcg atggccgacg cggtggtgga
     2641 tttgcccttg ctgcccgtga caccgatgat cgggttgcgc gcgatggaca ggaacaccga
     2701 gagatcggtc tcgatggcca cgtcgtgctc ccgggcggcc gtgagatagg gggagctcgt
     2761 cgccaccccg gggttcttga tcacgacgtc ggcgttgacg aagtcgctct cctcgtggcg
     2821 ccccaggacg tagcgaacgg gaaaggacgc cagccgagag atcgactctg ccagggcctt
     2881 ctcgtcccgt aggtccgtca ccgtgagcgt ggccccccgg cgtgcgaaga actgggcgga
     2941 ggcaaggccg ccgccgttca ccccgagacc catgacggtc acgcgcatgc cggggatgga
     3001 cccgggatca atcctcaatg cggtactcct tggtctcctg ttcgttgaaa taggcttcca
     3061 cgcggatgtt cttccagggc tcgtaccatt gttcgggcac gcgcgccttc agctcccgat
     3121 aatagccgat gtcctcggag aacgtggcgg tacgcatcac ggtctggatc cgatccatgg
     3181 ccggcagcag gaactggatg acgcggtgca gccacgggat ggtcgtcttg cggccctccg
     3241 gagccaggct cagtacggag tggcagatcg tcttgtactt gcagaggtac ggatagtaag
     3301 ggtaggtgcg cttgccctgg gggaggaaaa gacggctgaa gaacttggta aggtccgcgt
     3361 ccatccagat cccctggacg aaatagccgc ggccctgctc tccgatccag gtctgagaga
     3421 tggtctgccg cacctggaag ctcgtgatcc gtccgtcctc tctggtgacc gccgccagcg
     3481 gcaccacgca gttctccacg aagaggcgcc gggtccggta gtggggggtc acgtcgttgg
     3541 tgccccggtc gatcacggtg ctggtggccg ggcgcatggt gaggtccacg cgggccagga
     3601 agaggtagac gcagtcgtcg acccgaaaga gccggtagaa acagggcctc aggatctccg
     3661 cgggatcgaa atagtagatg agctctcgca gcacctgggg agccagagcc accacctgct
     3721 ccaccacgga tcccagcttc tccatgtacc gggccggcgg cttgggatca cgcacgtcgt
     3781 ggtggatggg gaactggggc acctcgaact cgcgctccaa caggaggaaa aagtcttcgc
     3841 cctggctgaa gtgccgcgtg aacggccgct ccccctcgcg gattccctcc agcagggcgt
     3901 tcagatcgcg atccgaagct ccaactctta tcgcgttgga tgtctgctcc accagcggaa
     3961 ctgtacgccg aaaccggcgc ctccggcaac ggccggggcg acgcggatcg gctcagaagg
     4021 cgctccgttg cggtccgtag cgcctcagga cgcgcgtgtc gccgcttccg ccatctgatc
     4081 cgggctcacc acggggccga agcgctcctt cagggccagg aggcccttct tctgctcgcc
     4141 atagatctcg aacaggcggg ccgggatcct cttctccttg cgataggcgt cctcctggag
     4201 ctggatcctc cccaggatgt tgtccacgta gaaggagaac tgcttgtcgt agagcgagcg
     4261 cgcgtagtcc ttggtcacca gggatcggaa gatcgtctgc aggtcctcat agcgcgggat
     4321 catgccgatg ggcgtctcga tggcctggac ctcgccgtgg gcgtagcgct ccagccagga
     4381 gagccatgcc ctcacgtccc gcttctctcc gaggagcttc accaggtcgc cgcctcgagc
     4441 gcagtccagg aggaagtagt tgaggccggc gatgagggga cggccctccg gggagaactt
     4501 cggcgagttg aagaactcga actgcgcctc catgtagtcg ccgagtggac acgggatgaa
     4561 cggggagttg gcccagggtt gtcggttcac ccccttcgcg cctacctccg tggccgtggc
     4621 cgccgatagg atggaggcgc cgatcaccac gccgtggtcc gggttcttgg ccacccacac
     4681 cggcggcatg gtgtcactgt cacggccgct gtaggtgatg acccggatag gcaccccccg
     4741 agggtcgtcc gccatcgtgg cgttgtagtt ggcgatggcg aggttggaga gcgtgatgcg
     4801 cgcgttgggg ttggagacgg gcaccggctt tccgttcttg tccgtcttgc cgggccacca
     4861 gtccccctgg aagttgcggc ccttcacggg aggagtctca ccgctaccgg tccaatgcgg
     4921 aacggagtcg aagtccacca ggacgttgga ccagatgacc tccgtgccgg gctctcgcag
     4981 gcacttgtag aggtaggggt caccctcacg gttgagatcc tcgacgatcc caaagatccc
     5041 cttttcggga ttcacggcgc ggagggtccc gtcctccgcg atccagatct gggaaagatc
     5101 gtcccccacg tagtccgtac cgaccatggc ggtggtcgtc ttgccgcagc cggaaggcgc
     5161 cgcgcccgcg aaaaacgtga cgcgcccgcc cgggccgcgc atccccgtga tgaacatgtg
     5221 ctcggagagc tcgctcccgc gccggtagta ggtgcagaga tccaccgcga accggtggtt
     5281 ccccttcttg agcatcaagg tgtttcccgc gtaggtgcag tacatgctga acgtcgtgaa
     5341 ccagcttcgg tccatgaaga tccgggcctt gccgatgtcc tcggagcggt tggtgccctg
     5401 gctgtgcacg ttggtgaaga agacgccggc ccgtttcgcc tccgcgtcga actgatcgta
     5461 cacgcaccga tagaggatgt tggcgctgtg caggacatag gcggagctgg agatcatcag
     5521 acccgggacc gccccctggg cgccgatggg gccgcggctg tagaacccga tcagcatcgt
     5581 cttgcccttc atgatgccct tcatggtggc gcggatgtag tccagcgcct ccgctcggag
     5641 catcttcttg gcaaggacgc tcacctcttc atcctcgttg gcgatgtaga aggtctggtc
     5701 caccatccgg ccctggtcct cgggcaggtc gaagtgcagg gtgtggtcct tcattgccag
     5761 ggggcactcc tcccccgtat ccagactgat ctggcggcag gcatccacgt cctcctggga
     5821 cgcggtgacc accagcacgc cgtccggcgc gcacatcgcg atggagttgg cgatccgcag
     5881 aagggcgtcc gggttcgtga tgcgggagag cttggcgcga ttgcgggagt ccaggctccg
     5941 gtccaggaac cgggtcgctt ccgagacggt tctcaggcct ccgacctcca ccaggatgtc
     6001 cacaccgctt ctcagcttca gcatagattc ctccccggtt ccgctctctc gtgcctaccg
     6061 cgcatagtta tgcatttttg tcatggaagc cggttccgcc tcgcgtgtca accggccggt
     6121 gccttacggg ccgcgaaatc tccgcgcgtg gatgcggtac ttccggaccc tcaggcccat
     6181 gatccgctcc gtgatcccca gggaggcagc cgcactggac atgttgccgc gggaagactt
     6241 cagcgcttcc aggagcagct cctgctccac acgctccaga gtggccttca gcgtcccgct
     6301 gggcaccgtt ccgctggcct cggcggtctg gagcgtcggc gggaggtgat ggccgtggat
     6361 cacgttgtcg tcgctgagaa ggaccgcgcg ctccacgcag ttctccagct cccgcacgtt
     6421 gcccggccag tggtaggcca tgagcatgtc gatggcggaa ttggagatcc ggcgcacgtc
     6481 ccggccgtgg gcgacggcgt acttctcgac gaaatggtcg g
//
LOCUS       DASRZS010000336         5236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1345863, whole genome shotgun sequence.
ACCESSION   DASRZS010000336 DASRZS010000000
VERSION     DASRZS010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345863"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VFH83_08195"
     CDS             complement(<1..879)
                     /locus_tag="VFH83_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486386.1"
                     /translation="MHAIEAHGLGKRYTNGVEAVRDLDLAVDPGEIFGFLGPNGAGKT
                     TTVRLLNGSLTPSAGTCRVLDVSSQDDASRALTATLTEQARMYEKLSVVENLRFYASM
                     YDEADHRGADSLGEDRIEELLHRMRLWEKRDAPLGTLSTGQRKRAQLARTLLHRPKVL
                     FLDEPTSGLDPDAALEVTTLIRDLAREAATTVFLCTHNLAMAERVCDRFGFLKMGMLV
                     AQGRTEELIRAATRERLVTIRTTRGAKDYPIERDDDINRRIREVLDQGLAILEVRQKL
                     PTLEDVYFEHIGRRSHE"
     gene            complement(896..1114)
                     /locus_tag="VFH83_08200"
     CDS             complement(896..1114)
                     /locus_tag="VFH83_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486387.1"
                     /translation="MNQHGGAINGVLFCAAAGLATVTGIALVIVGTWASRRGVDARRD
                     RTGRLLRRVVVAVAALSAAAATAGLLWR"
     gene            complement(1221..2132)
                     /locus_tag="VFH83_08205"
     CDS             complement(1221..2132)
                     /locus_tag="VFH83_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET6486388.1"
                     /translation="MNQWAVWFNVISFTLMFAAFGFSFLGYLQDRRPWFRSYLLYFAS
                     YAFWLLLATYVYFRVSFVPGEHASLDLVAGLLRVAVSLIIAYSAPLMVAQLSPSGASR
                     VIRVPLLIVPVLILLAAFLALRFTTAASAISGASSILFNGFMLAVAARGLTILRKSVP
                     DPRAASLRAFFRLSMVLYGALICYGLAARLILSSVLSAVSVFAIGAFGLAWSILVIVD
                     QLARAHRGVEDLRSLPALFVREYRITDREAATVQLLVEGKTTREIGEALFVSHRTVET
                     HTRNIYRKCRVSNKVELIKLIESCRAR"
     gene            complement(2224..3399)
                     /gene="fabV"
                     /locus_tag="VFH83_08210"
     CDS             complement(2224..3399)
                     /gene="fabV"
                     /locus_tag="VFH83_08210"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabV"
                     /protein_id="HET6486389.1"
                     /translation="MLIERRIRGGVCLSVHPHGCEELVEEQIAYVRSRPRLERGPRKA
                     LILGASTGYGLATRIAAAFGARADTVGVFYERPADGDRTASAGWYNSAYFEEQAHQDG
                     LNAWSLNGDAFSNPIKEQTLDLLRKHTGPVDLVVYSVAAPKRGAFSSTLRPIGKPFSS
                     KTVNLQTGQVYETSVPPATEEDIRGTVAVMGGEDWQLWIEALRGAGLLADGVITVAYS
                     YIGSSVTAPVYREGTIGRAKEHLEATALGLDASLKALGGRAVVAVNKAVVTQASAVIP
                     FVPLYIAILYRVMKELGTHEGSIEQMDRLLRDRLYGEGGIPVDEAGRVRLDERELDPE
                     VQRIVGERWRDVTTENLRQLADWDGYREELSRICGFDFPGVDYRVDVDAKVGIRSLG"
     gene            complement(3496..3888)
                     /locus_tag="VFH83_08215"
     CDS             complement(3496..3888)
                     /locus_tag="VFH83_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HET6486390.1"
                     /translation="MKLLERWKGAARRLRLETYTLYYAYRNRRTPWYARAWGALVVAY
                     ALSPIDLIPDFIPVLGLLDDLVLVPLGIAIAVRLIPKPVYQESQALAYQRIQEAKPNW
                     TMAILIIVIWATVASLIAWWILRKLKVT"
     gene            complement(3968..5173)
                     /locus_tag="VFH83_08220"
     CDS             complement(3968..5173)
                     /locus_tag="VFH83_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET6486391.1"
                     /translation="MVSIFFIAAIYLLISVVAAVIAYHLDVSIALVEICLGIAAAAVA
                     DRFFGTGSLAGSQPWLQFLASAGAVLLTFLAGAELDPAVMREKVREVSVVGLVGFLAP
                     FGGGTAVARFLLGWSVPASFLGGIALSTTSMAVVYAVMLETGLNKTEFGKGILGACFI
                     NDLGTVLALGLAFSPFTYKTIVFVGASAVMLVSLPTVTRFITRVYGNRTAAVRTKWII
                     AVLLGLGALAYWSGSEAVLPAYVVGIVLAEFASAEHQWLRRLRTLTTGFLTPFYFIRA
                     GSFVSVPALIAAPAAFLLLFLAKVLSKILGLYPVISRFRGQRQERWYYTLLMSTGLTF
                     GTISALYGFTHGIVTQAQYSALVATVIASAVIPTLIAGQVFLPRHLLETPARAHQTLR
                     IPEGSPADE"
BASE COUNT          977 a         1752 c         1728 g          779 t
ORIGIN      
        1 ctcatggctt ctcctcccga tgtgctcgaa atagacatcc tccagggtcg gcagcttctg
       61 cctcacctcc aggatggcaa gcccctggtc caggacctcg cgaatgcgtc gattgatgtc
      121 gtcatcccgc tcgatgggat agtctttcgc gccgcgggtg gtgcggatgg tgacgaggcg
      181 ctcccgggtg gccgcccgga tcagctcctc cgtccggcct tgagccacca gcatccccat
      241 cttgaggaac ccgaagcggt cgcacacccg ctccgccatc gccaggttgt gggtgcacag
      301 gaacacggtc gtcgcggcct cccgcgcaag gtcgcggatc agcgtggtca cctccagggc
      361 cgcatccgga tccaggccgg aggtcggttc atccaggaat aggaccttgg gccgatggag
      421 cagggtccgc gcaagctgcg ctctcttccg ctgacccgtg gacagcgtac ccagcggggc
      481 atcccgcttc tcccagagcc gcattcgatg gaggagctcc tcgatgcggt cttctccgag
      541 ggagtcggcg ccccggtggt ccgcctcgtc gtacatcgag gcatagaatc gaaggttctc
      601 gaccacggag agcttctcgt acatccgcgc ttgctcggtg agtgtcgccg tgagcgctcg
      661 ggatgcatcg tcctgggacg acacgtccag cacccggcac gtgccggcgg acggcgtcag
      721 gctgccgttc agcagccgca ccgtggtggt cttcccggcg ccgttgggac cgaggaagcc
      781 gaagatctcc cccgggtcca ccgccaggtc caggtctcgg accgcctcca ccccgttggt
      841 gtagcgcttg ccgagcccat gcgcctctat cgcatgcatc cgatcctcct tctcctcacc
      901 gccagaggag tccggccgtg gccgccgcgg cggagagggc agccaccgcc acgacgacgc
      961 gccgcagcag cctcccggtt cgatcccgcc tggcgtcgac gcccctgcgg ctggcccacg
     1021 tccccacgat caccagggcg attcccgtga cggtagcgag ccccgcggcc gcgcagaaca
     1081 ggaccccgtt gatcgcaccg ccgtgctgat tcatctcacg ggtaacgatg ccaggttccg
     1141 ggggcgttgc ccatccgtga aactaccgga tcggcccttg cgcggcgggg acctcgcttc
     1201 tacgtagttc tacggagccg tcagcgggcg cgacaggact cgatgagctt gatcagctcc
     1261 accttattgg aaacgcggca tttgcggtag atgttgcggg tatgcgtctc gacggtccgg
     1321 tgggagacga acagggcttc accgatctcc cgcgtcgtct ttccttccac caggagctga
     1381 acggtggcgg cctcccgatc ggtgatccgg tactcgcgca caaagagcgc cgggagggac
     1441 ctcaggtcct ccaccccacg atgggctctg gcaagctgat ccacgatcac caggatgctc
     1501 caggcgagcc cgaaggcgcc gatggcgaac acgctgacgg ccgagaggac ggaggagagg
     1561 atcagccgcg cggccaggcc gtagcagatc agcgccccgt acaggaccat ggaaagacgg
     1621 aagaacgcgc gcagggaggc ggcgcgcgga tccgggacgg acttccgcag aatcgtgagc
     1681 ccccgcgcgg ccacggccag catgaacccg ttgaagagga tgctcgaggc cccgctgatg
     1741 gccgacgccg cggtggtgaa gcgaagggcc aggaacgccg ccagcagaat gaggacggga
     1801 acgatgagca agggcacgcg gatgacgcgg gaggctcccg agggagagag ctgcgccacc
     1861 atcaacgggg ctgagtaggc gatgatcagc gagaccgcga cgcggagcaa tcccgccacg
     1921 aggtccagcg aggcatgctc tccgggaacg aagctgaccc ggaagtagac gtacgtcgcc
     1981 agaagcagcc agaaggcgta ggaggcgaag tagaggagat agcttcggaa ccacggcctg
     2041 cgatcctgca ggtagcccag gaacgagaag ccgaacgccg cgaacatgag cgtgaacgag
     2101 atgacgttga accagacggc ccactgattc atggcgcatc gtggccggtt cgaagagtgc
     2161 ggtcaatacg ccgcggggcg cgggtccggg tccgccccag cgccgcacgc atggcggcgg
     2221 gtttcagccc agagagcgaa tgcccacctt ggcgtccacg tccacgcggt agtccacgcc
     2281 cgggaagtcg aagccgcaga tccgggaaag ctcctcacga tacccatccc agtccgcgag
     2341 ctggcggaga ttctccgtgg tcacgtccct ccagcgctct ccgacgatcc gctgaacctc
     2401 gggatcgagc tcccgctcgt ccaaccgcac ccggccggcc tcgtccaccg gtatgccgcc
     2461 ctccccgtag aggcgatccc gaagcagccg gtccatctgc tcgatgctgc cttcgtgggt
     2521 gccgagctcc ttcatgaccc ggtagagaat ggcgatgtac aggggaacga acgggatgac
     2581 cgcgctggcc tgcgtgacca ccgccttgtt cacggcgacc acggcgcggc cacccaacgc
     2641 cttgagggat gcgtccaggc ccagcgcggt ggcctccagg tgctccttgg ctctcccgat
     2701 cgtaccctcc cggtagaccg gcgcggtgac cgacgagccg atgtaggagt acgccaccgt
     2761 gatcaccccg tcggccagaa gaccagcccc gcgaagggct tcgatccaga gctgccagtc
     2821 ctctccgccc atgaccgcca cggttccgcg aatgtcctct tccgtagcgg gcgggaccga
     2881 ggtctcgtag acctggccgg tctggaggtt gaccgtcttg ctggaaaagg gcttcccgat
     2941 gggccgcagg gtcgacgaga acgcgccgcg tttgggcgcc gccacgctgt aaaccacgag
     3001 gtccaccggg ccggtgtgct tgcgaaggag atcgagcgtc tgttccttga tcggattcga
     3061 gaatgcatcg ccgttgaggc tccaggcgtt cagcccgtcc tggtgcgcct gctcctcgaa
     3121 gtacgcgcta ttgtaccagc cggcggacgc cgttcggtcg ccgtccgcgg gccgttcgta
     3181 gaacacccct acggtgtccg ccctggcgcc aaacgccgcg gcgattcgcg tggccaggcc
     3241 ataccccgtc gatgcgccca ggatcagcgc cttccgggga ccccgctcca ggcgcggtcg
     3301 ggaccggacg taggcgatct gctcctccac cagctcctcg cagccatgcg ggtggaccga
     3361 cagacacaca ccccctcgaa tcctgcgctc gatcagcatg gtgggaagat acgcacacgg
     3421 cacccggaag gcgagccccg gggtgatcgg cgagagccga gatgagaata ccctccggga
     3481 cggtgaacgg aaggctcagg tcaccttcag tttcctcagg atccaccagg cgatcagcga
     3541 ggcaacggtt gcccagatca cgatgatgag gattgccatg gtccagttgg gcttcgcctc
     3601 ctggatgcgc tggtatgcca acgcttggct ttcctggtag accggcttgg ggatgagccg
     3661 taccgcgatc gcaatcccca gcggcaccag aacgaggtca tccaggaggc caaggacggg
     3721 gatgaagtcc ggaatcagat cgatggggct cagcgcgtag gcaacgacaa gggcccccca
     3781 cgccctggcg taccagggag tgcgccgatt gcggtaggcg tagtacaggg tgtatgtctc
     3841 gagccgcagc cttcgggccg cgcccttcca acgctccagc agcttcatag gccaggcgcc
     3901 tcccaccgta ccgcatccga ggcggcgcgg acagaggaag agccgcatcg cgcgccccgc
     3961 gcctccccta ctcgtcggcg gggctgcctt cggggatccg cagcgtctga tgggccctcg
     4021 cgggggtctc gagcaggtgc cggggcagga agacctgacc ggcgatgagg gtaggaatga
     4081 ccgcgctcgc gatgaccgtc gccaccagcg cggagtactg cgcctgcgtg acgattccat
     4141 gcgtaaagcc gtacagagcg gagatggtgc cgaaggtgag gcccgtggac atcagcagcg
     4201 tgtagtacca tcgctcctgc cgctgtccgc ggaaccgcga gatgaccggg tacagaccga
     4261 ggatcttcga cagcaccttg gccagaaaga gcaggagaaa ggcggccgga gccgcgatca
     4321 acgccgggac cgaaacaaac gagccggcgc ggatgaagta gaacggggtc aggaagccgg
     4381 tggtcagggt cctgagccgt cgcagccact ggtgctcggc gctcgcgaac tcggcgagca
     4441 cgatcccgac cacgtaggcg ggaaggaccg cttcgctccc ggaccagtac gccagggcgc
     4501 ccaggcccag caacaccgcg atgatccact tggttcggac cgcggcggtc cggttcccgt
     4561 agacgcgagt gatgaaccgc gtgacggtcg gcagggagac cagcatcacg gcgctcgccc
     4621 cgacgaacac gatggtcttg tacgtgaacg gtgagaacgc cagaccgagc gccaggaccg
     4681 tccccagatc gttgatgaag caggcgccga gaatgccctt tccgaactcc gtcttgttca
     4741 gcccggtctc gagcatcacg gcgtacacca cggccatgga cgtggtagag agggcaatgc
     4801 cgcccaaaaa gctcgcgggc accgaccagc cgagaaggaa ccgcgcaacc gcggttccgc
     4861 cgccgaaggg ggccaggaag ccgaccaggc ccacgacgga tacttcccgc accttctccc
     4921 gcatgacggc cgggtccagc tcggcgccgg ccaggaacgt cagaagcacg gcgcccgccg
     4981 aggccaggaa ctgcagccaa ggctggctgc cggccaggga gccggtaccg aagaagcggt
     5041 ccgccaccgc cgctgccgcg atccccaggc atatctccac cagggcgatg gagacatcca
     5101 gatgatacgc gatcacggcc gcgacaaccg aaatgagcag atagatagct gcgatgaaga
     5161 agatggatac catggggcca cctcctggcg ctggcaaccg atgtcaggta ggagtcatca
     5221 gccccacggg gcggtt
//
LOCUS       DASRZS010000337         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1346059, whole genome shotgun sequence.
ACCESSION   DASRZS010000337 DASRZS010000000
VERSION     DASRZS010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346059"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VFH83_08225"
     CDS             complement(<1..993)
                     /locus_tag="VFH83_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET6486392.1"
                     /translation="MTGYPFTEIERKWQKYWEDHKTFHAVEDPSVPREKRKYVLDMFP
                     YPSAAGLHVGHPEGYFATDIYSRYLRMNGYQVLHPMGFDSFGLPAENYALKTGTHPRT
                     STLQNIDRFTTQIKALGFSYDWERRISTCEPEYYHWTQWIFLKLWEKGLAYEAEAPIN
                     WCPSCQTGLANEEVRDGRCDRCDTPVVRRNVRQWILKITAFAERLLADLDLLDWSESL
                     KLMQRNWIGRSEGANVRFSLENGKDVIEVYTTRPDTLFGATYMVLAPEHPLVAKLTTP
                     AQRKSVDAYIAAAAQKSDLERTELAKDKTGVFIGVHAINPVNGKKIPVWISDYIL"
     gene            complement(1067..>2117)
                     /locus_tag="VFH83_08230"
     CDS             complement(1067..>2117)
                     /locus_tag="VFH83_08230"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET6486393.1"
                     /translation="LLLRGGPLLSGLRVASGLWILALIGLLLGGVPGALAGAMDRGSA
                     RGRVVLYEKDSPYSYIRVTDTPDALGVERTLRLDALVHNKTDPEHPDSLRYEYERIFE
                     AMTRFQAERLAVGGSAAVTAARFATLTLGGGGFTMPSYLERHYPEAHHAVVEIDPDVV
                     AAAHRYFGLSSTTGLDIEISDARRYVRSAGGRSWDIIYCDAFNAFSVPAHLTTREFTE
                     AVARILSPNGIYLANVIDVFDSGRFLAAYLDTVQSVFSSTAVYVDPSFRTYARATFVV
                     AAAGAPAAGVPAARASAAAFPAELRDPNGTLVGRELTATETADLRAHTHPLVLTDDRA
                     PVDTLMAPVFLRAVH"
BASE COUNT          356 a          690 c          727 g          344 t
ORIGIN      
        1 caggatgtag tccgagatcc agaccgggat cttctttccg ttcacggggt tgatggcgtg
       61 gacgccgatg aaaacgccgg tcttgtcctt ggccagctcg gtgcgctcca gatccgactt
      121 ctgggccgcg gcggcgatgt aggcatcaac gctcttcctc tgggccggcg tggtcagctt
      181 cgccaccagg gggtgctccg gcgccagcac catgtacgtg gcgccgaaga gcgtatccgg
      241 ccgggtggtg tagacctcga tgacgtcctt cccgttctcc agggagaagc ggacgttggc
      301 cccctcgctc ctgccgatcc agttgcgctg catgagcttc agggactcgc tccagtccag
      361 caggtcgagg tccgccaaca gccgttcggc gaaggccgtg atcttcagga tccactggcg
      421 cacgttgcgc cggaccacgg gagtgtcaca ccggtcacag cggccgtccc ggacctcctc
      481 gttggccagc ccggtctggc aggacggaca ccagttgatg ggcgcctccg cctcgtaggc
      541 cagccccttc tcccacagct tcaggaagat ccactgggtc cagtggtagt actccggctc
      601 acaggtggag atccgtcgct cccaatcgta cgagaagccc agcgccttga tctgggtggt
      661 gaagcgatcg atgttctgca gggtcgacgt tcgcgggtgg gtgcccgtct tcagggcgta
      721 gttctccgcc ggcagcccga aggagtcgaa ccccatgggg tggagcacct ggtacccgtt
      781 catccggagg tagcggctgt agatgtccgt ggcgaagtag ccctcgggat gtcccacgtg
      841 aagcccggcc gcggaagggt aggggaacat gtccaggacg tactttcgct tctccctcgg
      901 gaccgagggg tcctcgacgg cgtggaaggt cttgtggtcc tcccaatact tctgccactt
      961 gcgctcgatc tcggtgaagg ggtaacccgt catggcggca taataataac ccccggaccg
     1021 ggggtcaatc gcgcccaggg tcagtggtcg tggccggcct cccgggtcag tgcaccgcgc
     1081 gcaggaacac gggcgccatc agcgtatcca ccggagctcg gtcgtcggtg aggaccaggg
     1141 gatgggtgtg cgcccgcaag tcggccgtct cggtggccgt gagctcgcgg ccaaccagcg
     1201 tgccgttcgg gtcccgcagc tcggcgggga acgctgcagc ggaagcccgg gccgccggaa
     1261 cgccggccgc cggagcgccg gccgccgcta ccacgaacgt tgctctggcg taggtccgga
     1321 aactcgggtc cacgtacacg gccgtggagg aaaacacgga ttgcacggtg tccaggtacg
     1381 ccgccaggaa tcgcccggag tcgaagacgt cgatgacgtt cgccaggtag atcccgttgg
     1441 gagagagaat ccgcgcaacc gcctcggtga actcccgagt ggtcaaatga gccggcaccg
     1501 agaaggcgtt gaacgcgtcg caatagatga tgtcccagga tcggccaccg gcggaccgga
     1561 cataccggcg cgcgtcggag atctcgatgt ccagcccggt ggtcgaagac agcccgaaat
     1621 accgatgcgc cgcggcgacc acgtcggggt cgatctctac gaccgcgtga tgcgcctccg
     1681 ggtagtgccg ctccaggtag gagggcatgg tgaaccctcc gccgcccagc gtcaacgtgg
     1741 cgaaccgcgc cgccgtgacc gcggcggagc caccgaccgc cagccgctcc gcctggaaac
     1801 gagtcatcgc ctcgaagatg cgctcgtact cgtagcgcag ggaatccggg tgctccgggt
     1861 ccgtcttgtt gtggacgagc gcgtccaggc ggagcgtgcg ctcgaccccg agcgcgtccg
     1921 gcgtgtcggt cacgcggatg taggagtagg gggagtcctt ctcgtacagg accaccctgc
     1981 cccgggcaga gccgcggtcc attgcgcccg ccagggcgcc ggggactccg cccaacagca
     2041 gtccgatgag cgccaggatc caaaggcccg aggcgacccg cagtccggag aggagcggcc
     2101 cgccgcgcag gagcaga
//
LOCUS       DASRZS010000338         3524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4038992, whole genome shotgun sequence.
ACCESSION   DASRZS010000338 DASRZS010000000
VERSION     DASRZS010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4038992"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFH83_08235"
     CDS             <1..215
                     /locus_tag="VFH83_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486394.1"
                     /translation="MLYTGIILFGLGNEVKIFLAGLVLAYVVLYEAYAVYRHDKTLGQ
                     RPELMKEMAVRKAAAQGRKTAGARGA"
     gene            303..1502
                     /locus_tag="VFH83_08240"
     CDS             303..1502
                     /locus_tag="VFH83_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET6486395.1"
                     /translation="MKRLPLIIVALALAAFALTPVFGLTSAQQANLKASLAVDTSVLK
                     SADGQQLLAPSTDNRKVPARPADPNKLAETDPLHWYDMEWAGWNVTPTNIPTSPGDGA
                     IGKKVIMIVHGDHPWTTACILGAKKVADMYKWDFKALSPNWDKNVQDQLIDQAINEKP
                     DMILLIPLDAKGAVQQARKINAAGVPLHMFNTLPDNAALNFSLGWTGPDDFGQFRLLA
                     RAWADQLRKANPGAKSIGVAYVQHAPGGSPYFARSFGPISELATYAPEIKTLVMASPT
                     PATFDANTTMQLVSDWLTKYGKDLKGICASDDSAQSLGIFQALEKAGRTDVIVVAAGN
                     SKQGMDLVKAGKLYGITYQTAEGDGALAAQSAADWFNGKGYSFRKNLAQHIITKADVD
                     KFYPPQW"
     gene            1591..1914
                     /locus_tag="VFH83_08245"
     CDS             1591..1914
                     /locus_tag="VFH83_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HET6486396.1"
                     /translation="MTRYGSVIGIQPGKLSEYKRYHANAWPEVLAMIQACNIRNYSIY
                     HKDGLLFSYFEYVGTDYQADMARMAADPRTQDWWAIMKPLQKPLPTRADGEWWAEMEE
                     VFHTD"
     gene            1926..3104
                     /locus_tag="VFH83_08250"
     CDS             1926..3104
                     /locus_tag="VFH83_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6486397.1"
                     /translation="MKRTDNCLGKLARMDAALHHREGDRVPVSDFFWGGFLERWRREL
                     GLAADTDIYRYYDLDWTVTIPNMDPHVRPFEIVRESQEEVVVRTGYEAVIRKKFADPM
                     PAYLSFGTDSLEKMEAFRFDDPWDERRFFRAGDNQIAGVGDGFSRNSPAWIETVRSLH
                     PDIPVYGSICEGHEQLWRIIGTENVMLWIGLYPEELGRFIERVGTFVIELTKAEIKAA
                     DGLLDGMVIWGDVAYVNGMLFSPVFWRKHFRPIVAEQIRICHDAGLPVIYHGCGNASP
                     IYDDFIELGLDSYNPLEAKSGLNVVDLRRRYGHRMGFCGNMDALAWANAPMEEIHRQA
                     MTKLNAARGGGYIFQSDHSVPTNISAERYDYIVNLVRKHGRYPLSLGEYELPEVGSSR
                     "
     gene            3101..>3524
                     /locus_tag="VFH83_08255"
     CDS             3101..>3524
                     /locus_tag="VFH83_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486398.1"
                     /translation="MTSRERVLTAFAHREPDRVPAWCGASPDFWAAAKRRLGVDDEGL
                     KIRFGDDFRRVAARYAGPDLALSPGATWKSPFGIERQGIGYGQPLSHPLEHAASVRDI
                     QDYPWPDPEWMDVSHVREDAMRWSSQYAILGGDWSPFWH"
BASE COUNT          644 a         1164 c         1125 g          591 t
ORIGIN      
        1 agatgctcta cacggggatc atccttttcg gtctggggaa cgaggtgaag atcttcctgg
       61 cggggctcgt ccttgcctac gtggtcctgt acgaagccta cgcggtctac cggcacgaca
      121 agaccctggg tcagcggccc gagctcatga aggagatggc ggttcgcaag gcggccgccc
      181 aagggaggaa gaccgcgggc gcccggggcg cgtagcctga ggtcccgcgt ccctttggtt
      241 tcgatttccg ggccgccccg taggggctat taccggatgc tcatatcgaa aggaggcagt
      301 ctgtgaaaag actcccgctt atcattgtgg cgctcgccct cgcggcgttc gccctcacgc
      361 cggtcttcgg gctgacgtcc gctcagcagg ccaatctgaa ggcctcgctg gcggtggaca
      421 ccagcgtcct gaagtccgcc gacggccagc agcttctggc gccctcgacc gacaaccgaa
      481 aggttcccgc gcggccggcg gacccgaaca agctcgccga gacggatccc ctccactggt
      541 acgacatgga gtgggcgggc tggaacgtca ccccgacgaa catccccacg agccccgggg
      601 acggcgccat cggcaagaag gtcatcatga tcgtccatgg cgatcacccc tggacgaccg
      661 cctgcatcct tggcgccaag aaggtcgcgg atatgtacaa gtgggacttc aaggccctgt
      721 ctccgaactg ggacaagaac gtccaggacc agctcatcga ccaggccatc aacgagaagc
      781 cggacatgat cctgctcatc cccctggacg ccaagggcgc ggtccagcag gcgcgcaaga
      841 tcaacgcggc cggcgtcccc ctgcacatgt tcaacacgct gcccgacaac gcggcgctca
      901 acttctccct gggctggacc ggaccggatg acttcggtca gttccgcctc ctggcccgcg
      961 cctgggcgga tcagctccgc aaggcgaacc cgggcgccaa gtccatcggc gttgcctacg
     1021 tccagcacgc tccgggtgga tcgccgtact tcgcgcgctc cttcggaccc atctccgagc
     1081 tggccaccta cgcgccggag atcaagacgc tggtgatggc ctctccgact cccgccacct
     1141 tcgacgccaa cacgacgatg cagctcgtct ccgactggct cacgaagtac gggaaggacc
     1201 tcaagggcat ctgcgcctcg gatgactccg cgcagtccct cggcatcttc caggccctcg
     1261 agaaggccgg acgcaccgac gtgatcgtcg tagcggctgg aaactccaag cagggcatgg
     1321 acctcgtgaa ggccggcaag ctctacggca tcacctatca gaccgccgaa ggcgacggcg
     1381 ccctggcggc ccagtccgcc gcggactggt tcaacggcaa gggctacagc ttccgcaaga
     1441 acctggccca gcacatcatc acgaaggcgg acgtggacaa gttctacccg ccccagtggt
     1501 aagcagaggt cggtaacccg caagccgccc gcgccgcgga aacccgcggt tccgggcggc
     1561 ttcgttgatc cgccgcgtca ggaggccgca atgacccgtt acggttccgt catcgggatc
     1621 cagcctggga agctctccga gtacaagcgc taccacgcga acgcctggcc ggaagtcctc
     1681 gccatgatcc aggcctgcaa tatccgcaac tactccatct atcacaagga cgggctcctc
     1741 ttctcctact tcgagtacgt ggggacggac taccaggcgg atatggccag gatggcggca
     1801 gacccccgga cgcaggactg gtgggccatc atgaagccgc tgcagaagcc cctgcctacc
     1861 cgcgcggacg gcgaatggtg ggcggagatg gaagaggtgt tccacaccga ctgaggggag
     1921 ggcccatgaa gcggaccgac aattgcctgg gcaagcttgc gcgcatggac gcggccctgc
     1981 accaccgcga gggggatcgc gtcccggtga gcgacttctt ctggggaggg ttcctggagc
     2041 gttggcgtcg ggagctgggg ctggccgcgg acaccgatat ctaccgctac tacgacctgg
     2101 actggaccgt caccatcccc aacatggatc cccatgtcag acctttcgag atcgtgcggg
     2161 agagccagga agaggtcgtg gtccgaaccg gctacgaggc ggtcatccga aagaagttcg
     2221 cggatcccat gccggcctac ctttcgttcg ggaccgattc cctggaaaag atggaggcct
     2281 tccggttcga cgacccctgg gacgagcggc gcttcttccg agccggagac aaccagatcg
     2341 ccggcgtcgg cgatggtttc tcccgcaaca gccccgcctg gatcgagacc gtgcggtccc
     2401 tgcatccgga catcccggtc tatgggagca tctgtgaggg gcacgagcag ctctggcgga
     2461 tcatcggcac ggaaaacgtg atgctctgga tcggcctgta ccccgaggag ttgggccgat
     2521 tcattgaacg ggtcggtacc tttgtgatcg agctgaccaa ggcggagatc aaggcggcgg
     2581 atggcctcct ggatggcatg gtgatctggg gggacgtggc gtatgtgaac ggcatgctct
     2641 tctctcccgt gttctggcgg aagcatttcc gtcccatcgt agccgagcag atccgcatct
     2701 gccacgatgc ggggcttccc gtgatctacc acggatgcgg caacgcctcc ccgatctacg
     2761 atgatttcat agagctcggg ctggattcct acaacccgct tgaggccaag tccggactga
     2821 acgtcgtgga ccttcgccgc cggtacggcc accgcatggg cttctgcggg aacatggatg
     2881 ccctggcgtg ggccaacgcg ccgatggaag agatccaccg tcaggccatg accaagctca
     2941 acgcggcgcg ggggggcggc tacatcttcc agtccgacca ttcggtgccc accaacatca
     3001 gcgccgagcg atacgactac atcgtgaacc tggtgcggaa gcacggccgc tatccgctct
     3061 ccctgggaga gtacgagctt cccgaagtcg ggagctcgcg gtgacctctc gggagcgagt
     3121 cctcacggcg ttcgcccatc gagaaccgga ccgcgtcccc gcctggtgcg gggcatcgcc
     3181 cgacttctgg gcggcggcga agcggcgcct gggtgtcgac gatgagggcc tgaagatccg
     3241 cttcggggac gatttccgcc gcgtggccgc gcgctatgcg ggcccggatc tggcgctctc
     3301 accgggcgcg acctggaaga gcccctttgg catcgagcgt caggggatcg gatacggtca
     3361 gccgctgtcc caccctctgg agcacgccgc ttcggtccgc gatatccagg actatccatg
     3421 gccggacccc gagtggatgg acgtgagtca cgtccgtgag gatgccatgc gctggtcctc
     3481 ccagtacgca atcctgggcg gggactggtc gcccttctgg catg
//
LOCUS       DASRZS010000339         5768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1346439, whole genome shotgun sequence.
ACCESSION   DASRZS010000339 DASRZS010000000
VERSION     DASRZS010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346439"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /gene="rsmH"
                     /locus_tag="VFH83_08260"
     CDS             <1..563
                     /gene="rsmH"
                     /locus_tag="VFH83_08260"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET6486399.1"
                     /translation="LVNGAEPAELTDILFRFGEERFARPIVSRIARERAKAPITTAAR
                     LAQVVSAAVPEPYRHRRIHPATKTFQALRIAVNRELDQLEEGLRAAIRILSPGGRIGV
                     ITFHSLEDRIVKRFFRERGKDCTCPPEWPICQCGGKAELRIITRKPLTASEEEATRNP
                     ASRSAKLRVAEKPSAVEPGTAPRGEP"
     gene            560..853
                     /locus_tag="VFH83_08265"
     CDS             560..853
                     /locus_tag="VFH83_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486400.1"
                     /translation="MRRVAPALLCLAIPALLLLNAWQGSRYGELLDQVSALEDEQSRL
                     LRANQDAIARIAYEEAPERVAEKAAAVSGLVPADPANVLRVTVAGTPAAGGQP"
     gene            850..2295
                     /gene="murF"
                     /locus_tag="VFH83_08270"
     CDS             850..2295
                     /gene="murF"
                     /locus_tag="VFH83_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET6486401.1"
                     /translation="MSGPGQRLFDEGEVAAATGATPIHSGSSTVAGVAVDSRKVLPGS
                     AFFALPGERTDGHRFLGAAAQAGAVLLVVSADQAQARSAEIAELARRFHLSVLISRDT
                     LAALQDLARFHLGRLPAMTRVAVTGSNGKTTTKELIASILRQVAPTTANEGNLNSEIG
                     LPLACFEIAPTSRYTVFEMGINHPGEMETLADIVRPDVALITNIGVAHIGLLGSQEGI
                     AREKRRIFSKFDGRQIAVLPEAAPFKALLAEGVRGRIVYHGPSSTPGYEGSEPMGLDG
                     TIIRWEGLRVRFPLFGPHNLSNALAAISLSREMGIAVPAIRDGLEAARPLFGRSQVLT
                     GRLTLLADCYNANPDSMGSALDFVEAVPWQGRKVAVLGAMRELGDQTVDAHRALGARL
                     RATSCGLVFLLGPEMEEAWKAMEGSPAAGRSRWFSELEPLAGVLQSALHAGDLVLLKG
                     SRSLEMEQLLPSITGESAPAGHGAPGRVSTC"
     gene            2289..3359
                     /gene="mraY"
                     /locus_tag="VFH83_08275"
     CDS             2289..3359
                     /gene="mraY"
                     /locus_tag="VFH83_08275"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET6486402.1"
                     /translation="MLKLLYAQRGSLSFLNIFQYITFRAAYAAITALLLCFLLGPWVI
                     ARLRTIGKSSGPREDTPLTHQAKAGTPTMGGVLILLSIVVSILLWQDPDSIYTWILAF
                     AALGFGAIGFADDYLKVTRKTGIKAAAKVGAQFVVSIIVVTAIYLERGDSTTLLYIPF
                     LKRAVLDLSWGYIPFAVILMVGYSNAVNLTDGLDGLAIGLVIFVALALTVLAYLTGRA
                     DYADYLSIPFVKGGGEISVFCLAVVGASVGFLWFNAHPAEVWMGDTGSLSLGGMVGVT
                     AIILKKEILILILGGVFVLETLSVIIQVVSFQLTGKRVFKMTPLHHHFELSGWPESRV
                     VTRLWILGGLFAILGLSTLKIQ"
     gene            3375..4478
                     /gene="ftsW"
                     /locus_tag="VFH83_08280"
     CDS             3375..4478
                     /gene="ftsW"
                     /locus_tag="VFH83_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET6486403.1"
                     /translation="MERVEKERSDMVLIMLLVVMLGVGIALLLSASYSFSNRAYQDPL
                     YLVKRQLLWAAVGGAAGFLASLTPLELIRRFMPWILLATLLFALLPFVPSLGTQVLGS
                     RRWITVFGQSFQPSEIIKVTLVLYMASYFARRESDGLSLNSLIPPFVVTVAFSAIILV
                     QNDYSTAIFVLVTGLALVFFARVRLLHVLLVCGLTVPLGFLLLFTREHRVQRLLAFLD
                     TSANRAGAAYQIVNAKAALVSGGLWGRGLGRSAAKLGPVPMSHSDFIYAIIGEETGLL
                     GALFVLALFGLLAWKGFQIALRATDRFTSLAAFGVTVVLCLQALLNMTVAVGLVPTTG
                     VTLPFFSAGGSSLLATLLLCGLLVNLSRRVSHV"
     gene            4471..5445
                     /locus_tag="VFH83_08285"
     CDS             4471..5445
                     /locus_tag="VFH83_08285"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET6486404.1"
                     /translation="MSSEYAIRQRALESGAATPRAAGPRAGASDVRSLHRALRVVLVA
                     LCTGCTLVLFARFVASPLMTIRHVVVHSDVPLAESQVLSLSGLQGDERYYALPVDLLR
                     RRLEASPLVRRAVVQKVFPDTVRMTVWGRQPAALLLAQSDGKMLPVLVDGEGVLFRVG
                     ETGSDLDVPVISGIPIGSSRIGERLPQAYAGLLADLKVLRDRDPALFRLISELKVVPA
                     ADAASVPGTEASAPAAAPASTSDPAAPSAQDEADSPAGFDVQLYLTTSPVPVLMHGDI
                     DESLVKYTLMVTDLLSKQGVLKDIQALDFRSGNVVYRMKERPSPLVGR"
     gene            5448..>5768
                     /locus_tag="VFH83_08290"
     CDS             5448..>5768
                     /locus_tag="VFH83_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET6486405.1"
                     /translation="MASDQLIVGLDIGTTKVCAIIGEFSETGQLEIIGVGTTPSRGLR
                     RGVVINIESTVKSIQNAVESAELMSGREVHGVYTGVAGGHIEGINSRGVVAVTGKDRE
                     ITKED"
BASE COUNT          843 a         1965 c         1926 g         1034 t
ORIGIN      
        1 acctggtgaa cggggcggaa ccggccgagc tgaccgacat cctgtttcga tttggagagg
       61 agcgttttgc ccgccctatt gtctcccgaa tcgctcgaga gcgtgcgaag gcgcccataa
      121 ccaccgcggc ccggctggcg caggtggtaa gcgcggcggt gcccgagccc taccgccatc
      181 gccggatcca cccggcgaca aagacgttcc aggcacttcg gatcgcggtc aaccgcgagc
      241 tggaccagct cgaagaaggg ctgcgcgccg ccatccggat cctgagcccc ggaggcagga
      301 tcggggtgat caccttccat tccctggagg accggatcgt gaagcggttc ttccgggagc
      361 gcggcaagga ctgtacgtgc cccccggagt ggccgatctg tcaatgtggg ggcaaagccg
      421 agctgcgcat cattacgcgc aagccgctca cggcctctga ggaagaggcg acccgaaacc
      481 cggccagccg cagcgccaag cttcgcgtcg cggagaagcc ctcggccgtc gaaccgggca
      541 ctgccccccg gggggaacca tgagacgcgt ggcgccggcc cttctgtgcc tggccattcc
      601 cgcgctgctt ctgctcaacg cctggcaggg gagccgctac ggcgagcttc tggaccaggt
      661 aagcgctttg gaggacgaac agtcccgcct ccttcgggcg aaccaggatg ccatcgcccg
      721 gatcgcctac gaggaggcgc cggagcgcgt cgcggagaag gccgccgcgg tgtccggcct
      781 ggttcccgcc gatcccgcga acgtccttcg ggtgacggtg gccggcaccc cagcggctgg
      841 cgggcagcca tgagcggacc cgggcagagg ctcttcgacg agggggaggt ggcggcggcc
      901 accggggcca cgccgatcca ctccggctcg tccaccgtcg ccggtgtggc cgtggactct
      961 cggaaggtcc tcccgggcag cgctttcttc gccctccccg gcgagcggac ggacggccac
     1021 cgcttcctgg gagcggccgc gcaggccggc gcggttctcc tggtggtctc cgccgaccag
     1081 gcccaagccc ggtccgcgga gatcgccgag ctcgctcgtc ggttccatct gtcggtcctg
     1141 atctcgaggg acacgttggc cgcgctccag gacctggcgc ggttccacct cggccgactg
     1201 ccggccatga cccgcgtcgc cgtgaccgga tccaacggaa aaacgaccac caaggagctg
     1261 atcgcctcga ttctccgcca ggtcgcgccc accacggcga acgagggcaa cctgaactcc
     1321 gagatcgggc tccctctcgc ctgcttcgag atcgctccga ccagccgcta cacggtgttc
     1381 gagatgggga tcaaccatcc cggcgagatg gagaccctgg cggacatcgt ccgaccggac
     1441 gtggcgctca tcacgaacat cggggtggcc cacatcgggc tcctgggttc ccaggaggga
     1501 atcgcccgag agaagcggag gatcttctcg aagttcgacg gccgccagat tgcggttctt
     1561 ccggaggcgg cgccgttcaa ggcgctgctt gccgaagggg tgcgtggccg gatcgtttat
     1621 cacggcccgt cgagcactcc gggttatgag ggaagcgagc cgatggggtt ggatggaacc
     1681 atcatccgct gggagggact ccgggtccgc ttcccgcttt ttggccctca caacctgtcc
     1741 aatgccctgg ccgccatctc gctttcccgg gagatgggga tcgccgtccc ggcgatccgg
     1801 gacggcctgg aggccgcgag gcctctcttc gggcgttccc aggtcctgac cggtcggctc
     1861 acgctccttg ccgactgcta caacgcaaac ccggactcca tgggaagcgc cctggatttc
     1921 gtggaggcgg ttccctggca gggcaggaag gtcgcggtgc tcggcgccat gcgggagctg
     1981 ggggaccaga ccgtcgatgc gcatcgggcc ctgggcgcgc ggctgcgcgc cacctcgtgc
     2041 ggcctcgtgt tcctcctggg acccgagatg gaggaggcct ggaaggcgat ggaaggaagc
     2101 ccggccgccg gccgatcccg gtggttctcg gagctggaac cccttgccgg tgtcctccag
     2161 agcgccctcc acgcgggaga cctcgtgctg ctcaagggct cccggagcct cgagatggag
     2221 cagcttctac cttcgatcac gggtgagagc gcgcccgccg gacacggcgc gccggggagg
     2281 gtctccacgt gctgaagctc ctgtacgccc agaggggatc gctctcgttc ctgaacatct
     2341 tccagtacat aacgttccgc gctgcttacg cggccatcac ggcgctgctg ctctgcttcc
     2401 tcctgggtcc gtgggtcatc gcccgcctgc gcaccatcgg aaagagctcg ggtccccggg
     2461 aggacacccc gctcacgcac caggccaagg ccggaacgcc gaccatgggc ggcgtgctca
     2521 tcctgctctc catcgtggta tccatcctcc tgtggcagga ccccgacagc atctacacct
     2581 ggatccttgc cttcgcggcg ctggggttcg gcgccattgg cttcgcggac gactatctca
     2641 aggtgacccg aaagaccggg atcaaggcgg ccgcaaaggt cggagcgcag ttcgtcgtct
     2701 ccatcatcgt ggtcaccgcg atctacctgg agcgcggcga ttccacgacg ctgctgtaca
     2761 tcccgttcct caagcgcgcg gtgctggact tgtcgtgggg ctatatcccg ttcgccgtga
     2821 tcctcatggt cggatactcc aacgccgtga acctgaccga tggactggac gggctggcca
     2881 tcggcctggt catcttcgtg gccctggctc tgaccgtgct ggcatacctg accgggcgcg
     2941 cggactatgc ggactacctc agcatcccgt tcgtcaaagg cggcggtgag atctccgtgt
     3001 tctgtctggc cgtggtcggc gcgtccgtcg gctttctctg gttcaacgcc catccggcgg
     3061 aggtgtggat gggggacacc ggcagcctgt ccctcggtgg catggtgggg gtgaccgcga
     3121 tcatcctcaa gaaagagatc ctgatcctca tcctgggcgg cgtcttcgtg ctggagacgc
     3181 tgtccgtgat catccaggtg gtctccttcc agctcacggg caagcgggtg ttcaagatga
     3241 ccccgctgca tcaccacttc gagctgagcg gctggcccga gagccgggtc gtcacccgtc
     3301 tgtggatcct tggcggcctc ttcgccatcc tgggtctctc caccctgaag atccagtaga
     3361 ggcagggacg agcgatggaa cgcgtcgaga aggaacggag cgacatggtg ctcatcatgc
     3421 tgctcgtcgt gatgctgggg gtagggatcg cgctcctcct ctcggcctcc tattccttct
     3481 ccaaccgcgc ctaccaggat cccctctacc tggtgaaacg gcagctcctc tgggccgccg
     3541 tgggcggagc ggccggtttc ctggcgtcgc tcaccccgtt ggagttgatc cgccggttca
     3601 tgccgtggat cctgctggct acgctcctct tcgcgctcct tccgttcgtg ccgtccctgg
     3661 ggacgcaggt gttgggatcc cgacgctgga tcacggtgtt cggccagtcc ttccagcctt
     3721 ccgagatcat caaggtcacc ctggttctct acatggcttc ctacttcgcg cgtcgggaga
     3781 gcgacggcct gagcctcaac tccctgatac cgccgttcgt cgtcacggtg gccttctccg
     3841 ccatcatcct ggtccagaac gattactcca cggcgatctt cgtgctggtc accgggctgg
     3901 ccctggtgtt cttcgcccgg gtccggctgc tgcacgtcct gctggtgtgc gggctcacgg
     3961 tcccgctggg cttcctgctc ctgttcacgc gggagcaccg ggtccagcgg ctcctggcgt
     4021 tcctggacac ctcggccaac cgggcgggcg cggcctacca gatcgtgaac gcgaaggcgg
     4081 cgctcgtgag cggcgggctg tggggcagag ggctcggccg cagcgcggcc aagctcgggc
     4141 cggtccccat gtcccattcg gacttcatct acgcgatcat cggcgaggag accggactgc
     4201 tgggcgccct gttcgtcctg gcgctctttg ggctcctggc ctggaaaggc ttccagatag
     4261 ccctgcgcgc cacggaccgc ttcacgtcgc ttgcggcctt cggcgtcacc gtcgtgctct
     4321 gtctccaggc cctgctgaac atgaccgtgg cggtgggcct ggtccctacc acgggggtca
     4381 cgctcccgtt cttctccgcc ggcgggtcgt cgctcctggc cacgctcctg ttgtgcgggc
     4441 tgctcgtgaa tctgtcgcgg agggtctccc atgtctagcg agtatgcgat ccgccagcgg
     4501 gctctggaat ccggggccgc caccccccgc gccgccggcc ctcgagccgg cgcaagcgat
     4561 gtccgttcgc tgcaccgcgc cctgcgggtg gtgctggtcg cgctgtgcac gggctgcacc
     4621 ctggtgctct tcgcgcgatt cgtggcgtcg ccgctcatga ccatccgaca cgtggtggtg
     4681 cacagcgacg tccctctggc ggagagccag gtgctgagcc tttcggggct ccagggagac
     4741 gagcggtatt acgcgctgcc ggtggacctg ctccgcagga ggctcgaggc cagccccctg
     4801 gtccgtcgag ccgtggtcca gaaggtgttc ccggataccg tgcggatgac cgtctgggga
     4861 aggcagccgg cggcgctgct cctggcccaa tcggatggaa agatgctccc ggtgctggtc
     4921 gacggggagg gggtgctctt ccgggtcggg gaaaccggca gcgacctgga cgtgccggtg
     4981 atctcgggca tcccgatcgg ctcctcccgc atcggcgaga ggctcccgca ggcgtacgcc
     5041 ggtctgctcg cggacctcaa ggtcctgcgg gaccgcgacc ccgcgctctt tcgcctcatc
     5101 tcggagctca aagtggttcc cgccgcggac gccgcgtccg tgccagggac ggaggcttcc
     5161 gctcccgccg ccgcgcccgc gtcgacgtcc gaccccgcgg ccccctcggc ccaggacgag
     5221 gccgacagcc cggcgggctt cgacgtccag ctctacctca ccacctctcc cgtgccggtg
     5281 cttatgcatg gcgacatcga cgagagcctg gtcaagtaca ccctcatggt gacggacctc
     5341 ttgtccaagc agggtgtttt gaaggatatt caggcactcg acttccgcag cgggaacgtc
     5401 gtctaccgga tgaaggagcg gccctcgccg ctcgtgggga ggtagcggtg gcatccgatc
     5461 aactgatcgt cggattggac atcggcacca cgaaggtgtg cgccatcatc ggcgagttca
     5521 gcgagacggg ccagcttgag atcatcggag tggggaccac gccgtcgcgg ggccttcgcc
     5581 gaggcgtggt gatcaacatc gagtctaccg tcaagtccat ccagaacgcc gtggagtccg
     5641 ccgagctcat gtccgggcgg gaggtgcacg gcgtatacac cggcgttgcc ggcggccaca
     5701 tcgagggaat caactcccgc ggggtggtgg ccgtgactgg caaggaccgg gagatcacca
     5761 aggaggac
//
LOCUS       DASRZS010000340         9694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4424091, whole genome shotgun sequence.
ACCESSION   DASRZS010000340 DASRZS010000000
VERSION     DASRZS010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9694
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4424091"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VFH83_08295"
     CDS             <1..660
                     /locus_tag="VFH83_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486406.1"
                     /translation="GISVIIAWVIGMAAGTVLGWRRGRTVDDVGTPVALFLSQMPIYL
                     MALLLATVFVYAVAVFPSGGAYSADVKKAFTPQFIGSVIYYGLLPAISMVLVNFAGWS
                     LSQRALVINLLGEDYMRLAEAKGLPSRVLVSRYILRNTMLPQATGLALSLGFVLNGFF
                     LIEWIFRYPGIGRLFVASIRATDFNVLLGICVLTMVVVLLANLIMEFVYPFIDPRIRR
                     G"
     gene            666..1571
                     /locus_tag="VFH83_08300"
     CDS             666..1571
                     /locus_tag="VFH83_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486407.1"
                     /translation="MEAQVQPRVRTGGIWTFLRLLRESPRLTVGLVLLLILAAAAIVE
                     PVINNARLQGRLSTEMGDFDVHAPSSPAHPLGTDDSGRDVLALQFTGLRNSLLIGLVA
                     GGLGTAIAVIMACLGGYLGGRTEMIITGITNSVLIIPSWPIVAIIVLFVFKPGVLFMS
                     AVLAFFTWPFPTRVIMPQIASLRERPFIDLARMTGQGSWSIMFREIVPNFLPFIVLGL
                     TTSISGSIMAETGLRIIGLGPVQLVTLGQLINWTLYAGTLSQGYFAIALSPIILLVLV
                     FVALNFVNFGLEEVYNPRLKKITGL"
     gene            1584..2558
                     /locus_tag="VFH83_08305"
     CDS             1584..2558
                     /locus_tag="VFH83_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486408.1"
                     /translation="MLPLLEVKNLNVSVRTIAGTPRILDNVSLPVFEKEILGIAGESG
                     CGKSTVVETVLRLIVPPRFVESGQVLLRGRDLLPLSQGELQKIRWKEIAYVPQGSMNT
                     LSPVLKIEEQIVDSIIYHAPVSREQAREMAVAALRSVGMSPDVAKMYPHELSGGMRQR
                     VCIGLSTTLKPSVIMADEPVTALDVVMQRLNLEALAQLRDSHGITVVMITHDMACHAE
                     ICDRVCVMYAGRVAEIGETRALFGDPLHPYTQGLLRAVPSIENRHSVSIPGMAPSPAN
                     WPTGCRFHPRCPRAMDICRREAPALQEVAPGRLVSCHLHTGKAGTPNG"
     gene            2551..3549
                     /locus_tag="VFH83_08310"
     CDS             2551..3549
                     /locus_tag="VFH83_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486409.1"
                     /translation="MAEQQLLELRNITKTFSRAGLGLGGRKTVQALDNVTVTLPDKPQ
                     VCALVGESGSGKTTLSRIVIGLEQATAGSVLYKGREVKDWLRKDMKGYRREVQVIFQD
                     PYATYNPFYRVDRVLWGVVRKFGLTHGREEGERRITEALEAMGLRPREILGRYPHQLS
                     GGERQRIMLARVYLLRPKLLVADEPVSMLDASLRAMFLDHLKAFRDLGVSCLYITHDL
                     NVGYFIADQILILCLGRMVEWGESDHIVKDPLHPYTQELISSIPNPDPEKRWKERLQA
                     EKIGLNELRRATTGAGCIYAQRCKHVMEKCRHNNPPLKRVKGREGDVREVACFLYE"
     gene            3562..3942
                     /locus_tag="VFH83_08315"
     CDS             3562..3942
                     /locus_tag="VFH83_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008001697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6486410.1"
                     /translation="MADGSVVRVADEVPFQPVAAGTATRMQVLLGQAEGAPHFAMRRF
                     RMEAGGGMPLHTNQVEHEQYVLCGRARVAIGDTVHEVSAGKVLLIPAGVPHSYEVIEA
                     PFEFLCLVPNLADKIEVVRDSKGC"
     gene            3936..4832
                     /locus_tag="VFH83_08320"
     CDS             3936..4832
                     /locus_tag="VFH83_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486411.1"
                     /translation="MLSRGLLIVVALLWGRVALFADIRALLGRIDALHDEGRFADARQ
                     LSLDSVAAAPTGAERAELYVRAARETLKLGQQAEDRGTPAGEVLRICEDGEAYADAAI
                     QADPGNDLGYYWKAANMGRWGQVKGMLNALFMTGPVRDLLSQEVGINPRRSDAYSVLG
                     QLYRMLPGWPISFGNVDAAVSLERESVDLRVSQVRSGEERTMDYSFTVELARSLFQRN
                     WSAETRLKEQESKRARYQGAATALERGFDYEGIVTLPPFSDREEARRIVEQTITQMEA
                     MPRRTADDEETLADAKGLLKSW"
     gene            complement(4847..6184)
                     /locus_tag="VFH83_08325"
     CDS             complement(4847..6184)
                     /locus_tag="VFH83_08325"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6486412.1"
                     /translation="MSVGIGTREEDLRRDAYLRGGASLIMWAFALAAFLLGLIHVRHL
                     VGVSAAVVYLVVLGFPTVAAARRIRGARAYAWFVVLVGVAEVIGYTAVIYSLGGVEAA
                     YLLPIYVTYVSYMGAVEHRESPYISAALCAFLFGLMVTLQDLGILPNMRAYVGFTLPV
                     PNQIVIAGAVSMLLFVTAYISASASGAINRMRRQLSEQNRELERRVEERTQELTREIE
                     ERKRAQEQISASLRDKEVLIKEVHHRVKNNLQVVSSLLSLQASFIQNPKTREMFAESE
                     ARVRSMALIHEMLYQGKGQERVEVSRYLEGVLSALSRSYGVTPDGIRFAVDAEGISLG
                     VEKAIPCGLIITELVSNALKHAFPGGRKGLVTVSVRAEPGGGNADATPIRLTVSDDGV
                     GLPGDLDLQHTETLGMQLVTTLAAQLGGSIEVTSQHGTSVTVLFSAGDAEAAP"
     gene            complement(6271..7929)
                     /locus_tag="VFH83_08330"
     CDS             complement(6271..7929)
                     /locus_tag="VFH83_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6486413.1"
                     /translation="MSIRAKIVLIVLPLIIVPLVLTGVISSLSARNGITTLATGFLQF
                     KAEQLVTYAQGQWDLLAANGLESTPEYLDASKAAIESFAKSLIRSPTEAIFALGPDGA
                     IALRSGDLSVSAPEMEALRALVGSGAAGWQTLNVGGQSRIAHLAGFTPFRWTFFVTES
                     AGSFYRSADAIVRQTAYMLAASILIALVLLLYFAQYLTRPLRQVADAMAEIISTNDLS
                     RRVDVLYKDETGRLGHTFNLMSEELGKAYDQVKGYALHAAVAQSKEQKIRHIFQMYVP
                     KNVIDEFFASPDKMLVGANRDLAVLFSDVRDFTGISEKMKPDEMVESLNQYFAPMVEI
                     IMNMGGIVDKYMGDGILAFFGAPLSSDRDALTSVQAGLDMLDSLTSFNAWQTQRGRRP
                     FRIGIGINFGSVTVGNIGSPRKMDYTVMGDNVNLASRLEGLTKKYHTPLIVSESVYPQ
                     IRGRLKCRRLDRVAVLGRSAGTVIYEVRRSITATQEEAWGLHERALDVYYQGRFAEAE
                     RAFRDVLSRDPEDHCARLFHERCQTFLRTPPPPDWDGIEEMRTK"
     gene            8044..>9694
                     /locus_tag="VFH83_08335"
     CDS             8044..>9694
                     /locus_tag="VFH83_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486414.1"
                     /translation="MRVSGLLRKAQSLRGDHPHLRLLRPDELTGGPVEDIPDDERQRI
                     LAQIDEAVASNRISVTAETFDFKPLRRGSFLPVVVNVAAVVVVAAGIGLAFFLSRRTE
                     SQIVTAPVALLSAETKMVQALKEQSRQQLDDKDKEIASIRTRLAGADQERARIQQEAD
                     ASVAQREKELESSMATALDAERRKLVASGMTEATVNKRIADLEAKSRADVDAQLAAFR
                     AQADKDRADREKALAAIQTEYQKSLAQAQADRARLQQDSAKQQADLQAGFQQKQLALE
                     KDKAAALAELDRLRQQQNQEQLVQDQILTYYQKARDAVQSGQPAAAQKVLADLRKYLG
                     DPTLAALPGIARRRPVDLFLADSLEQLIQSQAAPSAATPIAPDLLASANLITTVASLV
                     QQGDSLFQEQGYSRARELYLSALARIPAVQAGYAKLDAIQKIYADRQRKDVADLLDAA
                     NTAYRAGNFDAAVDRYGRALEQLQLDRGGVDTLIQELVDIGGQRKASQDAVALKGLQD
                     GATSRSRSAAGVAALRSRLAAAAAPTQSDAGRDTLVALVATK"
BASE COUNT         1674 a         3150 c         3241 g         1629 t
ORIGIN      
        1 gggatctccg tcatcatcgc gtgggtgatc ggcatggcgg cgggcaccgt gctggggtgg
       61 aggcgaggcc ggacggtgga tgacgtcggc acgccggtcg ccctgtttct gtcccagatg
      121 ccgatctatc tcatggcgct gctcctggcc accgtcttcg tctacgccgt ggcggtcttc
      181 ccctccggcg gcgcgtactc ggcggacgtg aagaaagcgt tcacgcccca gttcatcggc
      241 agcgtgatct actacggact gctccccgcc atatccatgg tgctcgtcaa cttcgccggc
      301 tggtccctct cccagcgcgc cctggtcatc aacctcctgg gggaagacta catgaggctg
      361 gcggaggcta aggggctgcc cagccgggtg ctggtcagcc ggtatatcct gcgcaacacg
      421 atgcttcccc aggctaccgg gctggccctc agcctgggat tcgtcctgaa cgggttcttc
      481 ctgatcgaat ggatcttccg gtatccgggc atcggcaggc tcttcgtggc ctccattcgg
      541 gccacggatt tcaacgtcct cctggggatc tgcgtgctca cgatggtcgt ggtcctgctg
      601 gccaatctga tcatggagtt cgtctacccc ttcatcgatc ctagaatccg cagagggtga
      661 gcggaatgga agcacaagta caaccacgcg ttcgaacggg cgggatctgg acgttcttgc
      721 ggctcctcag ggaatccccg cgactcacgg tcgggcttgt cctcctgctc attctggcgg
      781 cggccgccat cgtggagccc gtgatcaaca acgcgcgcct ccagggtcgg ctgtccacgg
      841 agatggggga cttcgacgtg cacgccccca gctcccccgc gcatcccctg ggcacggatg
      901 actccggcag ggacgtactg gcgctccagt tcaccggtct tcgcaactcg cttctgatcg
      961 ggctggtggc cggcgggttg ggaacggcca tcgccgtgat catggcctgc ctgggaggat
     1021 acctgggcgg acgcacggag atgatcatca ccggcatcac caactcggtg ctgatcatcc
     1081 catcgtggcc catcgtggcg atcatcgtcc tgttcgtgtt caagcccggg gtcctcttca
     1141 tgtccgccgt cctggccttc ttcacctggc ctttccccac ccgggtcatc atgccgcaga
     1201 tcgcgagcct tcgagagcgt cccttcatcg acctggcgcg tatgaccggc cagggaagct
     1261 ggtccatcat gttccgggaa atcgtgccga acttccttcc cttcatcgtc ctgggcctga
     1321 ccacgtcgat ctccggctcc atcatggcgg agacgggact ccgcatcatc ggtctgggtc
     1381 cggtccagct cgtcacgctg ggccagctca tcaactggac gttgtatgcg gggacgctgt
     1441 cccagggata cttcgccatc gcgctcagcc ccatcatcct gctggtgctg gtgttcgtgg
     1501 cgctcaactt cgtcaacttc gggctggagg aggtctacaa cccccgtctg aagaagatta
     1561 ccgggctcta gagagggggc gccatgctgc cactgctgga ggtgaagaac ctgaacgtct
     1621 ccgtccggac catcgccggg acgccccgga tcctggacaa cgtctccttg ccggtcttcg
     1681 agaaagagat cctggggatt gccggggagt ccggctgcgg aaagtccacg gtggtcgaga
     1741 cggtcctgcg actgatcgtc ccgccccggt tcgtggagag cgggcaggtc ctgctgcgcg
     1801 ggagggacct gcttccgctg agccagggag agttgcagaa gatccgctgg aaggagatcg
     1861 cctacgtccc tcagggctcc atgaacacgc tcagcccggt cctcaagatc gaggagcaga
     1921 tcgtggacag catcatctac catgcccccg tcagccggga acaagcccgg gagatggcgg
     1981 tagccgcgct gaggtccgtg gggatgtctc cggatgtggc caagatgtat ccccacgagc
     2041 tctcgggtgg gatgcgccag cgggtgtgca tcgggctcag cacgaccctc aagccttccg
     2101 tgatcatggc ggatgaaccg gtaacggcgc tggacgtggt gatgcagcgg ctgaacctgg
     2161 aggcgctggc gcagcttcgg gatagccacg ggatcaccgt ggtgatgatc acccacgata
     2221 tggcctgcca cgcagagatc tgcgaccggg tctgtgtcat gtacgcgggg cgggtggcgg
     2281 agatcgggga gacccgggcc ctgttcggcg atcctctgca tccgtacacc cagggcctgt
     2341 tgcgagcggt tccttccatt gaaaaccgcc attcggtgag catccccggc atggcgccca
     2401 gccccgccaa ctggcccacc ggctgtcgct tccatcctcg gtgtccgcgg gccatggaca
     2461 tctgccgcag agaggcgcca gcccttcagg aggtcgcgcc gggccggctg gtgtcctgtc
     2521 acctccacac cggaaaggcg gggacgccca atggctgagc agcagctcct ggagcttcgc
     2581 aacatcacca agaccttctc ccgagccggc cttggccttg gcggcagaaa gacggtgcag
     2641 gcgttggaca acgtcaccgt cacgcttcca gacaagcccc aggtgtgcgc cctggttggc
     2701 gagagcggaa gcggcaagac cacgctgtcc cggatcgtca tcgggctgga gcaggccacc
     2761 gccggttccg tgctctacaa gggccgagag gtcaaggact ggctccgcaa ggacatgaag
     2821 ggctaccgcc gggaggtcca ggtcatcttc caggacccct acgccaccta caaccccttc
     2881 taccgcgtgg accgggtcct ctggggcgtg gtgaggaagt tcggccttac ccacggccgg
     2941 gaggaaggag agcggaggat cacggaggcc ctcgaggcca tggggcttcg gccgcgggag
     3001 atcctggggc gctatcctca ccagttgagc ggcggtgagc ggcagcggat catgctggcg
     3061 cgcgtctacc tgctgcgccc caagctgctg gtggcggacg agccggtatc gatgctcgac
     3121 gcttcgctgc gggccatgtt cctggaccac ctgaaggcgt tccgggacct gggggtgagc
     3181 tgtctctaca tcacccacga tttgaacgtg ggttacttca tcgcggacca gatcctgatc
     3241 ctctgtctgg ggagaatggt ggagtgggga gaatcggacc acatcgtgaa ggatcctctc
     3301 catccctaca cccaggagct catctcttcc attcccaatc ccgatcccga gaaacgatgg
     3361 aaagagagac tccaggcgga gaagatcggg ctcaacgagc ttcggagagc caccaccggc
     3421 gccggctgca tctacgccca gcggtgcaaa cacgtcatgg agaagtgccg ccacaacaat
     3481 ccccctctga agcgcgtgaa gggccgggag ggcgacgttc gagaggttgc ctgtttcctg
     3541 tacgagtagc ggaggagagc tatggccgac ggatccgtgg tgcgcgttgc cgatgaggtg
     3601 cccttccagc ccgtggcagc cggaacggca acccggatgc aggtcctcct ggggcaggcg
     3661 gaaggcgctc ctcacttcgc catgcggcgc ttccgcatgg aggcgggcgg cggcatgccc
     3721 cttcacacca accaggtcga gcacgagcag tacgtcctgt gcggacgggc acgggtggcc
     3781 atcggggaca cggtccatga ggtctccgcg ggcaaggtgc tgctgatccc ggcgggggtc
     3841 ccgcattcgt acgaggtcat tgaggcgccc ttcgagttcc tgtgcctggt cccgaacctg
     3901 gcggacaaga tcgaggtcgt gcgggactcc aagggatgct gagccgcggg ctcctgatcg
     3961 tggttgcgct tctctggggc cgcgttgcgc tcttcgcgga catccgcgct ctgctcgggc
     4021 gcatcgacgc gctgcacgac gaggggcgat tcgcggacgc ccgccagctt tccctggact
     4081 cggtcgcggc cgcacccacc ggcgcggagc gcgcggagct ctacgtgcgc gcggcgcgag
     4141 agacgctcaa gctgggccag caggcggagg accgcggcac cccggccggg gaggttctgc
     4201 ggatctgcga ggacggtgaa gcgtacgcgg acgccgccat ccaggcggac ccgggcaacg
     4261 acctgggcta ctactggaag gccgcgaaca tgggccggtg gggccaggtc aagggcatgc
     4321 tgaacgccct cttcatgacc ggcccggtcc gcgatctcct gagccaggag gtggggatca
     4381 acccacgcag atccgatgcc tacagcgtac tgggccagct ctaccggatg ctccctggct
     4441 ggccgatctc cttcggcaac gtggatgcgg ccgtgtccct ggagcgcgag tccgtggacc
     4501 tgagggtctc ccaggtccgc tcgggggagg agcgcaccat ggactacagc ttcacggtgg
     4561 agctggccag aagtctcttc cagaggaact ggtccgccga aacccggctg aaggagcagg
     4621 agagcaaaag agcgcggtac cagggcgccg cgaccgcgct ggagcggggg ttcgactatg
     4681 aggggatcgt caccctcccg ccgttctccg accgcgaaga ggcccggcgc atcgtggagc
     4741 agaccatcac ccagatggag gcgatgccgc ggcggacggc ggacgacgaa gagacgttgg
     4801 ccgacgcgaa ggggctcctc aagagctggt aggctggagc tcctcgtcag ggagccgctt
     4861 cggcatctcc cgcggagaag aggacggtga cgctggtccc gtgctgggag gtcacctcga
     4921 tggagccgcc gagctgggcg gccagcgtgg tgacgagctg catccccaac gtctcggtgt
     4981 gctgcaggtc caggtccccc ggaaggccca ccccatcatc gctcacggtg aggcggatgg
     5041 gagtggcgtc tgcgttccca ccgccgggct cggcgcggac cgagacggtg accaggccct
     5101 ttcggcctcc cgggaacgca tgcttcagcg cgttggacac gagctccgtg atgatgagcc
     5161 cgcaggggat tgccttctcg acgcccaggg agatcccttc cgcgtcgacg gcgaaccgga
     5221 tcccatcggg cgtgacgccg taagaccgag agagcgcgct cagcacgcct tccagatagc
     5281 ggctcacctc tacccgttcc tggcccttgc cctggtagag catctcgtgg atgagggcca
     5341 tcgagcggac ccgtgcctcg ctctccgcga acatctcccg ggtcttcgga ttctggatga
     5401 agctggcttg aaggctgagg aggctcgaga caacctggag gttgttcttg acccggtggt
     5461 gcacctcctt gatcaggacc tccttatccc gcagcgaagc ggatatctgc tcctgggcgc
     5521 gcttgcgctc ctcgatctcc cgcgtgagct cctgggtccg ttcctccacg cgccgctcaa
     5581 gctcccggtt ctgctccgag agctggcgcc tcatgcggtt gatggcgccg gaggccgagg
     5641 cggagatata ggcggtcacg aacaggagca tggagaccgc gccggcgatc acgatctggt
     5701 tgggcaccgg aagcgtgaag ccgacgtacg cccgcatgtt cggcaggatg ccgaggtcct
     5761 gcagggtcac catgagaccg aagaggaacg cgcaaagagc cgcggagatg tagggcgact
     5821 cccgatgctc gacggcgccc atgtaggaca cgtaggtgac gtagatgggc aggagatacg
     5881 ccgcctccac gcccccgagg gagtagatga ccgcggtgta gccgatcacc tccgcgacgc
     5941 ccaccagcac gacgaaccag gcgtatgcgc gggcgccgcg gatccgacgg gcagcggcca
     6001 cggtcgggaa ccccaggaca acgagataga cgaccgcggc gctgaccccc acgaggtgtc
     6061 ggacgtggat caaccccagc aggaacgcgg cgagggcgaa cgcccacatg atgaggctcg
     6121 cgccgccgcg caagtaggcg tctctgcgga ggtcttcttc ccgggtcccg atcccaacgc
     6181 tcatgctcaa accctcaccg aagcgcacat tgtttatgcc tcacaatccg cccgaaggaa
     6241 cgacgcttcc ccgagcttca acgacgttcg ctacttggtt ctcatctcct cgatcccgtc
     6301 ccagtcgggt ggcggaggcg ttctcaggaa tgtctggcag cgctcatgga acagccgcgc
     6361 gcagtgatcc tccggatcgc gggagagaac gtcgcgaaac gccctttccg cttccgcgaa
     6421 cctgccctgg tagtagacgt ccagcgcgcg ctcgtggagg ccccaggcct cttcctgcgt
     6481 cgccgttatg gaacggcgga cctcgtagat caccgtgccc gcgcttcggc ccaggactgc
     6541 cacgcggtcg agccttcggc acttcagtct gcctcggatc tgagggtaga cggattcgga
     6601 cacgatgagt ggggtgtggt acttcttggt gagcccctcc aggcgggacg ccaggttcac
     6661 attgtccccc atgaccgtgt agtccatctt gcgcggggaa ccgatgttcc ccaccgtgac
     6721 ggagccgaag ttgatcccga ttccgatgcg gaagggccgg cgtccgcgct gggtctgcca
     6781 cgcgttgaag ctggtcaggc tgtccagcat gtccagtccg gcctggacgc tggtgagcgc
     6841 atcccggtcg ctgctcagcg gcgctccaaa gaaggccagg attccgtccc ccatgtactt
     6901 gtcgacgatc ccacccatgt tcatgatgat ttccaccatc ggggcgaagt actggttgag
     6961 ggactcgacc atctcgtccg gcttcatttt ctccgagatc cccgtgaaat cgcgaacgtc
     7021 ggagaacagc acggccaggt cgcggttcgc tcccaccagc atcttgtcgg ggctggcgaa
     7081 aaactcgtcg atgacgttct tgggcacgta catctggaag atgtgccgga tcttctgttc
     7141 cttggactgc gcaacggcgg cgtgcagagc gtatcccttg acctgatcgt acgctttgcc
     7201 cagctcctcg ctcatgaggt tgaaggtgtg gcccaggcgg ccggtctcgt ccttgtacag
     7261 gacgtccacc cgacgcgaca ggtcgttggt ggagatgatc tccgccatgg cgtccgccac
     7321 ctgcctcaag gggcgcgtca ggtactgcgc gaagtacagg agcaggacca gggcgatcag
     7381 gatggaggcg gccagcatgt aggcggtctg gcggacaatg gcgtccgcgg agcggtagaa
     7441 cgatccggcc gactccgtga cgaagaaggt ccagcggaag ggcgtgaagc ccgccaggtg
     7501 cgcgatgcgg ctctgtccgc caacgttcag ggtctgccaa cccgcggcgc cggaccccac
     7561 cagggcccga agggcttcca tttccggagc gctcacgcta agatccccgc tgcgcagcgc
     7621 gatggcgcca tccggcccca gggcgaagat ggcctccgtg ggggaccgga tgaggctctt
     7681 cgcgaagctc tcgatggccg ccttcgaggc gtccaggtat tccggcgtag actccaggcc
     7741 gttggcggcc agaaggtccc actggccttg cgcgtaggta acgagctgtt ccgccttgaa
     7801 ctggaggaat cccgtcgcca gcgtcgtgat gccgttcctc gccgagaggg acgagatgac
     7861 gcccgtaaga accaacggta caatgatcag tggcaggacg atgaggacga tcttggcccg
     7921 aatgctcacc gcgaaccaga atagcccggc cccgctctgg tggtcaacgc gcgcgcgttg
     7981 cgcgccgcgc tggatcttac gggcccgcgt gccgatggta cgggaaaggc cttaccggcg
     8041 accatgcgcg tgagcggact gcttcgcaaa gcccagtctt tgcgcggaga tcatccccac
     8101 cttcgactcc tgcgtcccga cgagctgacc ggcggcccgg tggaagacat cccggacgac
     8161 gagagacagc ggatcctggc ccagatcgac gaggccgtcg ccagcaaccg catctcggtg
     8221 acggcggaga cattcgactt caagccgctt cggaggggca gcttcctgcc ggtcgtggtg
     8281 aacgtggcgg cggtcgtggt cgtagcggcc gggatcgggc ttgcgttctt cctctcccgc
     8341 cgcacggaaa gccagatcgt caccgccccg gtcgccctcc tcagcgcgga gaccaagatg
     8401 gtccaggcgc tcaaggagca atcccgccag cagctcgacg acaaggacaa ggagatcgcc
     8461 tccattcgga cccggcttgc cggggcggac caggagcggg cacggatcca gcaggaggcg
     8521 gacgcctcgg tggcccagag ggagaaagag ctggagagct cgatggcgac ggcgctggac
     8581 gccgagcgca ggaagctggt ggccagcggg atgacggagg ccacggtgaa caagcggatc
     8641 gccgacctgg aggccaagag ccgcgcggat gtcgacgctc agctcgcagc cttccgagcg
     8701 caggcggaca aagaccgcgc ggaccgcgag aaggcgcttg ccgccatcca gaccgagtac
     8761 cagaaatccc ttgcccaggc gcaggccgac cgcgcgcggc tccagcagga ttcggcgaag
     8821 caacaggcgg acctccaggc cggtttccag cagaagcagc ttgccctgga gaaggacaag
     8881 gccgcggccc tggcggagct ggaccggctt cggcagcagc agaaccagga gcagcttgtc
     8941 caggaccaga tcctgaccta ctaccagaaa gctcgggacg ccgttcagtc cggccagccc
     9001 gcagccgctc agaaggtcct cgcggatctg cggaagtacc tgggtgaccc taccctggcg
     9061 gcgctgccag ggatcgctcg acgcaggccc gtggacctct tcctggcgga ctccctggag
     9121 cagctcattc agtctcaggc ggctccgagc gccgcgacgc ccatcgcgcc ggacctgctg
     9181 gcctcggcga acctcatcac gacggtggca tccctggtcc agcagggcga ttcgctgttc
     9241 caggagcagg ggtacagccg cgcccgggag ctgtacctgt cggccctggc gcgcatcccc
     9301 gcggtccagg ccggctacgc gaagctcgac gccatccaga agatctatgc cgatcgccag
     9361 cgcaaggacg tggcggacct cctggatgcc gccaacacgg cgtaccgcgc cgggaacttc
     9421 gacgcggccg tagaccgcta tggccgggcc ctggagcagc tccagctcga ccgaggcggc
     9481 gtggacacgc tgatccagga gctggtggac atcggtggcc agaggaaagc gtcccaggac
     9541 gcggtggcgc tcaaggggct gcaggacggc gcgacgagcc gcagccgaag cgcggcgggc
     9601 gtagcggcgc ttcggtcccg gctggcggcg gccgcggccc cgacgcagtc agacgccgga
     9661 cgggatacgc tggtcgcgct ggtggctacc aagc
//
LOCUS       DASRZS010000341         3987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3269025, whole genome shotgun sequence.
ACCESSION   DASRZS010000341 DASRZS010000000
VERSION     DASRZS010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3269025"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..3829)
                     /locus_tag="VFH83_08340"
     CDS             complement(164..3829)
                     /locus_tag="VFH83_08340"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HET6486415.1"
                     /translation="MRQGQSELLPEYFSFNRDLDNLFVESGRQAHVIWRLDGSFGGLR
                     AVSKEIVNPPGGGTEGIWAEPLRLADYVHFHVWDSSAGLTPLERNAKRYTRNLGSSEV
                     FYQFGSHPYLSVLKTVWVPLERPAVIVELDVRNESDGERSVRLFAEYQSHLSIGWPLR
                     EGGRNSAGYDAAIPGIVSKDETHPEWAGICTCSHLPAAYHLGDFAPHLIGAGTLPSRA
                     GGTREPGPGRSCMQFDLTLPPEQSLRLAFVVCGSPRSEEEARGIATEVTSRLAELREE
                     KRSHYERLFVDTAVLESPSYVVNKAFLWAKLGTEDFKHLDPRLGYLFFAGYPAYNFYF
                     ASDSMLILRGSLAIGDFDDARRMLRTIVRYQATERGRDTLPGEIWHEMSTTGDRISPN
                     FAGFLFPGLMAAFHDWTGDDEFLREMYPHVRALVEWGYGMDTNGDGLLENGPQGEMAD
                     SASEDRNVERSHYHVQVQWLDALQEGARLADRVGDSPSATRWRETRQKLTPLVNRFYW
                     NESERYFEETLRPDGCLDTSGKGYANLDAGMVDEGKVFSTTRLLLEEEAYLTDIESFR
                     ARKEFEKTFSTHRSYMSWYVMVRGRRALDLYRAHRPEPASRALEDIAASPFHWTTPGL
                     WPEVWAVDEPSTLRARGCFHQAWTGSHGFIYPLVEGMLGLRPDAAHNALALDPHLPAH
                     WPSLRLRKVRMGRGWLDVECEQEAGVRRLTVRNQGADPLTVSFGFALPLWARVLRLTV
                     DGKERELTDLRVEQGPQDQHACVTAAAAPGGASTAELSWDRAPISFDLAPPSPAILEP
                     TKGDGATLLPRAAPGSTVPVRFEVGNHGRRRCRVRLELDTPGGWAVNGKPARSMVIGP
                     EQTRTEEFLVRIPPETAESYYTLRARLVGNPAVPAARTAYLPVFRSLSAAVDARPVAR
                     AGMPFTIQVPVVNLSPADKDVEVRVDVPKEWQSAGTGSQRLTLKPGQKAIATLGAVPG
                     GDGDTAVRVVVSAGDPRDQRDQWELSHPVTVLPPNRPLVLFSGFLGCPLPRSETAEVV
                     NLPANYALRRPHVLDQLLPMTTLLLTSDQHDAVLTPQQVASIIRFVREGGRLLFYCCW
                     SAPWGRGFYDTFGSMAQSDLPQTLPLHYRRDILHAAAVRLTQTGERVFSSVPWGTIPA
                     FDYNAADLLPGARLLAATPDGAPLIADWSFGKGRVLAIAIDCFGFESYVEGLSFDFWE
                     GKPRLIADGVRWLLGSE"
BASE COUNT          676 a         1349 c         1333 g          629 t
ORIGIN      
        1 ctcgttcttt cccctggtcc gggtctgcta cctcgaggag tccgccgtgg cggaggtcga
       61 ccctctgggt agatcgttcc tcaacgtgaa ctccccggcg gactacgaag cccttcgctc
      121 cgtcgtgcag gacgatcagg acgcggggcg ggcgtgaacg ggatcactcg ctccccagaa
      181 gccaccgtac tccgtccgcg atgaggcgcg gcttgccctc ccaaaagtcg aagctgaggc
      241 cttccacgta agactcgaac ccgaaacagt cgatcgcgat cgccagcacc cggcccttcc
      301 cgaagctcca atccgcgatc agcggcgcgc cgtccggcgt agccgccagg agacgcgcgc
      361 cggggagaag atcggcggcg ttgtagtcga acgcggggat cgtgccccaa gggacggagg
      421 agaaaaccct ctctccggtc tgggtgaggc ggaccgccgc ggcgtgcagg atgtcccgtc
      481 ggtaatggag cgggagcgtc tggggcaggt cgctctgcgc catcgatccg aacgtgtcgt
      541 agaatcctct cccccagggc gcggaccagc agcagtagaa gagcaggcgg cctccctccc
      601 ggacgaaccg gatgatggag gcgacttgct gcggcgtcag gaccgcgtcg tgctgatcgc
      661 tggtcaggag aagggtcgtc atgggtagaa gctgatccag cacgtgaggt cggcgaaggg
      721 cgtagttggc aggcaggttg accacctcgg ccgtctccga gcgcggaagc ggacagccca
      781 ggaaaccgga gaaaaggacc aaaggcctgt tgggcggcag aacggtcacc ggatgggaaa
      841 gctcccattg gtcccgctgg tcccgcggat ccccggcgct caccacgacc cgcaccgccg
      901 tgtcaccatc accgcccggt accgctccca gggtcgcgat ggccttctgt ccgggcttga
      961 gggtgagcct ctgggaacca gtcccagcgc tctgccactc cttgggcacg tcgacccgta
     1021 cctcgacgtc cttgtccgcc ggggagaggt tgaccactgg aacctggatg gtgaacggca
     1081 tgccggcccg agccaccggg cgcgcgtcaa cggccgccga cagcgagcgg aacaccggca
     1141 ggtaggcggt gcgggccgcc gggacggcgg gattcccgac gagccgcgcc cgaagcgtgt
     1201 agtaggattc cgccgtttcc ggcgggatac ggaccaggaa ctcctccgtg cgagtttgct
     1261 cgggcccgat gaccatcgac cgcgcaggct tgccgttcac ggcccacccg ccaggcgtgt
     1321 ccagctccag gcggaccctg catcgacgcc gcccgtggtt gccgacctcg aagcgaacgg
     1381 gaaccgttga cccgggggca gcccgaggca gcagcgttgc tccgtcgcct ttcgttggct
     1441 cgaggatcgc cgggctgggc ggcgccaggt cgaaggatat cggcgcacgg tcccagctca
     1501 gctccgccgt gcttgccccg ccgggcgccg cggcggcagt cacgcaggcg tgctggtcct
     1561 gcggcccctg ctcaacgcgc agatccgtga gctcccgctc cttgccatcg acggtgagcc
     1621 gcaggactct ggcccagagg ggcaacgcga atccgaagct cacggtcaac ggatccgcgc
     1681 cctggtttct aacggtcagc cgtcggactc ccgcctcctg ctcgcactcc acgtccagcc
     1741 acccgcggcc catgcggacc ttgcgcagcc gcagggacgg ccagtgggcg ggcaggtggg
     1801 ggtccaacgc cagcgcgttg tgggccgcat ccggcctcag tcccagcatg ccctccacca
     1861 acgggtagat gaagccgtgg ctgccggtcc aggcctggtg gaagcagccg cgcgcgcgaa
     1921 gcgtgctggg ctcatccacg gcccacacct cgggccagag gccaggggtg gtccagtgga
     1981 acggggaggc ggcgatgtcc tccagcgcgc gggaggcggg ctccggccgg tgggcgcggt
     2041 acagatcgag ggcgcgtctt ccccgcacca tcacgtacca ggacatgtag gaccggtgcg
     2101 tgctgaaggt cttctcgaac tccttgcgag ctcggaagga ctcgatgtcc gtgaggtacg
     2161 cttcctcctc caggagcaat cgggtggtcg agaaaacctt gccctcgtcc accatgccgg
     2221 catccaggtt cgcatacccc ttgccggagg tgtccaggca accgtccggg cggagcgtct
     2281 cctcgaagta gcgctcggat tcgttccagt agaaacggtt cacgagcggc gtaagcttct
     2341 gccgggtctc ccgccaacgg gtggcgcttg gggagtctcc cacccggtcc gcgagccggg
     2401 ccccctcctg gagagcgtcc agccactgca cctgaacgtg gtaatgggac cgctcgacgt
     2461 tgcggtcctc cgaggcggag tccgccatct cgccttgcgg tccgttttcc aggaggccgt
     2521 ccccgttggt gtccatcccg taaccccatt cgaccagggc gcggacgtgc ggatacatct
     2581 cccgcaggaa ctcgtcgtcg cccgtccagt cgtggaacgc ggccatgagc ccgggaaaca
     2641 ggaagcccgc gaagttgggc gagatgcggt ccccggtggt gctcatctcg tgccagatct
     2701 caccggggag cgtgtccctg cccctctccg ttgcctgata ccgcacgatg gtccggagca
     2761 tccggcgggc gtcgtcgaag tcgccgatgg ccagggaccc tcgcaggatg agcatggagt
     2821 cgctggcgaa gtagaagttg taggccgggt agccggcgaa aaagaggtac cccagccggg
     2881 gatccaggtg tttgaagtcc tcggtgccca gcttcgccca gagaaaggcc ttgttgacca
     2941 cgtagctggg ggactccagg acggcggtat caacgaacag ccgttcgtag tgggagcgct
     3001 tctcctcccg gagctcggcc agacgggacg tcacctccgt cgcgatcccg cgcgcctcct
     3061 cctccgaccg agggctcccg cagaccacga acgcgagccg cagggactgt tccggcggga
     3121 gcgtcaggtc gaactgcatg caggaccggc ccgggcccgg ctcccgcgtg ccgcccgccc
     3181 gggagggcaa cgtccccgcc ccgatgaggt gcggggcaaa gtccccgagg tgataggccg
     3241 ccggcaggtg agagcaggtg cagatcccgg cccactcggg atgcgtctcg tctttcgata
     3301 cgatgccggg gatcgccgcg tcgtagccgg ccgagttgcg cccgccctcg cgaagaggcc
     3361 agccaatgct cagatgggac tggtactcgg cgaagagacg cacggaccgc tccccgtcgg
     3421 actcgttccg aacgtcgagc tcgacgatca ccgccggacg ctccagcggc acccacacgg
     3481 ttttcagaac gctcaggtac gggtgggagc cgaactggta gaagacctcg ctggagccga
     3541 ggttccgggt ataccgtttt gcgttgcgct ccaacggagt cagcccggcg ctggagtccc
     3601 agacatggaa gtggacatag tccgccaggc ggagcggctc cgcccagatt ccttccgtac
     3661 cgccaccggg gggattgacg atctccttgg aaacggcccg cagaccgcca aagctcccgt
     3721 ccagcctcca gatgacgtgc gcctggcggc cggattccac gaagaggttg tccaggtccc
     3781 ggttgaacga gaagtactcg ggaagaagct cgctctgccc ttggcgcatc tcgacgctcc
     3841 cttcgtgacg tgatgtccgc ggtgctccac gctgtcacaa ccgggattcg gtgtcaacga
     3901 taactccaaa gactccacca ccggaagcgg caggacaacc caaaaacctt gacttcgacc
     3961 cgctttctgg tagtatcagc tcatcag
//
LOCUS       DASRZS010000342         4009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_964146, whole genome shotgun sequence.
ACCESSION   DASRZS010000342 DASRZS010000000
VERSION     DASRZS010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964146"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1457
                     /locus_tag="VFH83_08345"
     CDS             57..1457
                     /locus_tag="VFH83_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET6486416.1"
                     /translation="MIRTRTSGPGIAVLAALTIAVITGCAVRGSPPHASANGAATSPG
                     VSASGGAGGTQGRSGAGGSQGAPAGFGSIAVLAAIARVGPLTAGNSTAATVAPIMTST
                     VASQVAGVVATVSHLAGDWVRKGDPVVLLDTSQLKLAVDNAQASLASAKIAYAVAQDS
                     TSQDDPKLTLQVQSAQSAVDSAQKNYDSQKALLDLGGTPASSVDQALSQLQQAQANLK
                     AAQTALEQNKKSDVWTLQQSRLAIDQAQVQLETVELNLQNASISAPFGGQIAAVTVNP
                     GMYVSQNTPVFTIVTEGKEITFSIPPADAPNLPVGTSVGFSYQGSTWAVKVSQAPVAP
                     LNGVVPMVAAVPSSFPVPYGAVGTVTYSLTVAHGAVVPIAALQTAEDQTYVFTIQNGK
                     AAVSNVTIVGQSGTMAAVSGIPDGSQLIVSPPPGLLAGSAVQVNAAAAAAPSSAPSAG
                     GGAPLLAAAPGAGASK"
     gene            1454..>4009
                     /locus_tag="VFH83_08350"
     CDS             1454..>4009
                     /locus_tag="VFH83_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6486417.1"
                     /translation="MKTLRDYETRIFNRVNPLVNFSVTRYVMAVGIFVAIVVFGIVST
                     LGLGVDLLPNIAIPAVVVRTSYPGATPSVMDLQVTQVVENVVSAISGITDINSASSQG
                     TSRVVLTFDPSTDKYADANQVATAVSAAVKSLPASASTPTIQTFDPNAAPIIQFGMAG
                     QGADLTDVNDYVTNSLAPQLERIDGVATILVDGGPARQFEVNLNPERLRYYNLNPQDV
                     VNAITSSALQMPIGTIVKNRTSLTFSTQNQPADISQIGETLVDSGRGIHVDQLGSVWE
                     SPSSPNYARVNGRPEVLVSVQKTTDANAVAVASSVRKALTAASLPAGYAVEISNDTTG
                     PIRASVDSTYRELFITAIVVAIIVLLFLGKLNTAFVVILAIPIALSASPILYRLAGFS
                     FNLVSLLALIIAIGIVVDDSIVVSENVERYRAMGFGLKESVLKGASEVFSAVVAASLS
                     LLSVLLPVSFIGGFIGAYLQQFSLGLAAAVAFSLLEAVLFLTVRLAYTPESKTQTWGD
                     FLRAWILLPDAIRWGWRSWRKALGVVAGIAAAVLILVLTHRPVFLLAMVAYPAALGLL
                     YYAVRVVLSFLQALTTVLHGWTEAGLEWVRDRYTAALSRLVGQGVWILVGCAAFLVTI
                     VVFVAPHIAFNFVPNTDSGVMSVSVRTPPGTPLEVTNDDVGRVEDYLRRQPEVVTVQS
                     VIGSSPNGVSGIFSGTNTANLVVQLKPVQDRISVFRLIPRYRAGILSRFHDQPSAQVL
                     LSAAGGFGGGSTLQLSVVSPDFNLLMRRNAPILQTLRRDPYVSDAYSTLSDTSLENDF
                     TPDPSRLKGTGITPAAIAAALQTYASGVQASTVVSGGLSYPIQVQADPTRLSGL"
BASE COUNT          622 a         1442 c         1251 g          694 t
ORIGIN      
        1 ggacgccctt gtctttgcct gccaccccta cccatcgcat ccgtaggagt cccaccatga
       61 tcagaacacg aaccagcggt cccggcatcg ccgtgcttgc cgcgctgacg atagccgtca
      121 tcacgggatg cgccgttcgg ggctcgccac cgcacgcttc cgcgaatggt gccgcgactt
      181 cccccggcgt ctccgcatcc ggtggcgccg gcggaaccca gggccgttcg ggcgcgggcg
      241 gttcccaggg cgctccggca ggcttcggct ccattgcggt gctggccgcg attgcgcgcg
      301 tcgggcccct taccgccggc aacagcacgg cggctaccgt ggcgccgatc atgacgagca
      361 ccgtcgcctc ccaggtggcc ggcgtcgtgg ccaccgtctc acacctggcc ggagactggg
      421 tcaggaaggg ggaccccgtg gtgctcctcg acacctccca gctcaagctg gccgtggaca
      481 acgcccaggc gtccttggcc agcgcgaaga tcgcctatgc cgtggcgcag gacagcacca
      541 gtcaggatga tccgaagctc acgctccagg tgcagtccgc ccagtccgcc gtggacagcg
      601 cccagaagaa ctacgactcc cagaaggcgc tcctcgacct cggcggaacg ccggcgtcat
      661 ccgtggacca ggccctgagc cagctccagc aggcgcaggc gaacctcaag gccgcgcaga
      721 ccgcgctgga gcagaacaag aagtcggacg tctggacgct ccagcagtcc cggttggcca
      781 tcgaccaggc gcaggtccag ctcgagaccg tggagctcaa cctccagaac gcgtccatca
      841 gcgcgccctt cggtggccag atcgccgcgg taaccgtgaa cccggggatg tacgtcagcc
      901 agaacacccc ggtattcacc atcgtgaccg agggcaagga gatcaccttc agcataccgc
      961 ccgcggatgc ccccaacctt ccggtcggaa cgtccgtcgg gttctcgtat caggggagca
     1021 cctgggcggt gaaggtcagc caggcgcccg tggcaccgct gaacggggtc gttcccatgg
     1081 tggccgccgt gccttcgtcg ttccccgtcc cctatggcgc ggtggggacc gtgacctact
     1141 ccctcacggt ggcccacggc gccgtcgtcc ccattgccgc tctgcagacc gcggaggacc
     1201 agacgtacgt cttcacgatt cagaacggga aagccgccgt ctccaacgtg accatcgttg
     1261 gtcagtccgg aacgatggcg gccgtctccg ggatccccga cggcagccag ctcatcgtga
     1321 gcccgccgcc ggggctgctc gccggctcgg cggtccaggt caacgccgcg gctgccgccg
     1381 ctccttcgtc cgcgccgtcg gcaggcggag gcgcgccgct gctcgcagcc gcgcccgggg
     1441 cgggagcctc gaagtgaaaa cgctgcgcga ctacgagacc aggatcttca accgggtcaa
     1501 ccccctggtc aacttctccg tgacccggta cgtcatggcg gtcggcatct tcgtggccat
     1561 cgtggtgttc ggcatcgtgt ccacgcttgg tctcggggtc gatctgcttc ccaacatcgc
     1621 aattccggcc gtcgtggtga ggacctcgta tcccggcgcc acccccagcg tcatggacct
     1681 ccaggtgacc caggtggtgg agaacgtggt ttccgcgatc agcggcatca cggacatcaa
     1741 ctccgcaagc tcccagggca ccagccgtgt cgtccttacc ttcgacccca gcacggacaa
     1801 gtacgcggac gccaaccagg tagccacggc ggtctccgcg gccgtgaaga gcctgcccgc
     1861 aagcgccagc acgccgacca tccagacctt cgatccgaac gccgctccca tcatccagtt
     1921 cgggatggcc ggacaaggcg ccgacttgac cgacgtgaat gactacgtga cgaactcgct
     1981 cgcgccccaa ctcgagcgca tcgacggcgt ggccaccatc ctggtggacg gggggccggc
     2041 ccggcagttc gaggtgaacc tcaacccgga gcgtcttcgg tactacaacc tcaatcccca
     2101 ggacgtggtg aacgccatca cgagctcggc gctgcagatg ccgatcggga cgatcgtcaa
     2161 gaaccggacg tccctcacct tctccacgca gaaccagccc gccgacatct cgcagatcgg
     2221 cgagaccctg gtggattccg gtcgcgggat ccacgtggat cagcttggga gcgtctggga
     2281 aagcccgtcg tcgcccaact acgcgcgcgt gaacggcaga cccgaggtgc tggtctccgt
     2341 gcagaagacc accgacgcga acgcggtggc cgttgcctcc tccgtccgca aggcgctgac
     2401 cgccgccagc ctcccggccg ggtacgcggt ggagatcagc aacgacacca cggggcccat
     2461 ccgggcgtcg gtcgactcca cgtaccgcga gctcttcatc acggcgatcg tcgtcgccat
     2521 catcgtgctc ctgttccttg gcaagctgaa caccgcgttc gtggtcatcc tggcgatccc
     2581 gatcgccctc tccgcgtccc ccatcctgta tcggctcgcc ggcttctcgt tcaacctcgt
     2641 ctccctcctg gcgctcatca tcgcgatcgg catcgtggtg gatgactcca tcgtcgtttc
     2701 ggagaacgtg gaacggtatc gggcgatggg gttcggcctg aaggaatccg ttctgaaagg
     2761 cgccagcgag gtattcagcg ccgtcgtggc ggcgtccctt tccctgctct ccgtgctcct
     2821 gcccgtgagc ttcatcggag ggttcatcgg cgcttacctc cagcagttct ccctggggct
     2881 cgcggccgcc gtggccttct ctctgctgga agcggtgctc ttcctcaccg tccgcctggc
     2941 gtacaccccg gagtccaaga cccagacctg gggcgacttc cttcgcgcgt ggatcctcct
     3001 gcccgacgcg atccgttggg gctggcgttc gtggaggaag gcgctgggcg tggtcgccgg
     3061 gatcgccgct gcggtcctga tcctcgtgct gacccatcgc ccggtcttcc tcctggcgat
     3121 ggtggcgtac ccggcggcgt tggggctgct gtactacgcc gtgcgggtcg tcctgtcgtt
     3181 cctccaggct ctcacgaccg ttcttcacgg ctggacggaa gcggggctgg agtgggtgcg
     3241 ggaccgctac accgcggccc tttcgcgcct ggtcgggcag ggcgtctgga tcctggtcgg
     3301 gtgcgcggcg ttcctggtga ccatcgtggt gttcgttgct ccccacatcg cattcaactt
     3361 cgtacccaac accgactccg gggtgatgag cgtcagcgtg cggacgcctc cgggcacgcc
     3421 cctggaggtc accaacgatg atgtgggccg ggtggaggac tacctgcgcc gccagccgga
     3481 ggtggtcacc gttcagtccg tcattggcag cagccccaac ggggtgagcg ggatcttctc
     3541 gggaacgaac accgcgaacc tggtggtgca gctcaagccg gtccaggatc ggatcagcgt
     3601 gtttcgtctc attccccggt atcgcgccgg cattctctcc cgcttccatg accagccgtc
     3661 ggcgcaggtc ctgctcagcg cggcgggcgg cttcggaggc ggctccacgc tccagctcag
     3721 cgtcgtttcg ccggacttca atctcctgat gcggcgcaac gccccgatcc tgcagaccct
     3781 ccgaagagac ccgtacgtct cggatgccta ttcgaccctg tcggacacga gcctcgaaaa
     3841 cgacttcacc ccggatccgt cccggttgaa gggaaccggg atcacccccg ccgcgattgc
     3901 cgccgccctc cagacctacg cctccggcgt ccaggcctcc accgtggtgt ccggcggtct
     3961 cagctatccc attcaagtgc aggccgatcc gacgaggctt tcgggcctc
//
LOCUS       DASRZS010000343         3393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_964380, whole genome shotgun sequence.
ACCESSION   DASRZS010000343 DASRZS010000000
VERSION     DASRZS010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964380"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFH83_08355"
     CDS             complement(<1..504)
                     /locus_tag="VFH83_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6486418.1"
                     /translation="MRDDVLLEVTGLRKHFPIKKGFLKRVVGHVRAVDGLSFSIRAGE
                     TLGLVGESGCGKTTAGRTILRAIEPTAGSVRMRVDGAPVEITTLARDELRRIRRHMQM
                     IFQDPYGSLNPRMTVREIIGEPLFVNGMARGRDLEDRVQTLMKDVGLRAEYMRRYPHA
                     FSGGQRQR"
     gene            complement(497..1507)
                     /locus_tag="VFH83_08360"
     CDS             complement(497..1507)
                     /locus_tag="VFH83_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486419.1"
                     /translation="METLLEIRNLKTYFFLDHGTVHAVDGVSCDLHRGRTLGMVGESG
                     CGKSVTARSILRIVGARGRIVEGKILLHGEGEAGIDLTAMDPKGREIRAIRGKTIAMI
                     FQEPMTSLSPVYTIGNQIMEAILLHQHTGKKEARELAIEMLWKVGISKPRQRVDEYPH
                     QLSGGMRQRAMIAMALSCRPKLLIADEPTTALDVTIQAQILDLMRNLKDEFGMAILMI
                     THNLGVIAEMAEEVAVMYLGRLVEEASSDDLFYDPRHPYTRALLRSIPKVGKKSGVPL
                     ESIRGVVPDPLNVPRGCPFHPRCRSFLRGTCEKSIPPLLEIAPGHRVACHLFVKEGIA
                     HA"
     gene            complement(1518..2723)
                     /locus_tag="VFH83_08365"
     CDS             complement(1518..2723)
                     /locus_tag="VFH83_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486420.1"
                     /translation="MDDRPSGRDEAQPYPAEPPSEPAPGVEGAEGGGADEEPLGEERI
                     FVATQWQLMWWKFRRHKVALASAIILIVIYLMSITCEFLAPYDPLVYDKRYFFAPPQR
                     IGFVTKAGFTLRPVVYRYTIGYDENLLRVYKPDVEHAYPIHLFVHGNPYKMWGLVRTD
                     LHLFGITDPDGTMFLLGTDRLGRDMLSRTLYGLRLSTSIGLVGVFLSFVLGILIGGFS
                     GYYGGFFDTVVQRIIEFVICMPQIPLWMGLSAALPPHWPAVRVYFGITIILSLVGWTG
                     LARVVRSKFLALREEDFVMSARLIGARETRIVLKHMVPSFFSHIIASITMSVPGMILA
                     ETALSFLGLGLRPPAISWGVLLQDAQNIKAVAQAPWLLIPGVFVVVTVLAFNFLGDGL
                     RDAADPYAR"
     gene            complement(2732..>3393)
                     /locus_tag="VFH83_08370"
     CDS             complement(2732..>3393)
                     /locus_tag="VFH83_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486421.1"
                     /translation="LFTLIFTWAVSFPIGIYSATHQYSLGDYTFTMIGFIGLATPSFL
                     IALVFMWIAFSVFNQSVGGLFSPDFIQAPWSLAKALDLLRHLWMPALVIGLSGTAGLI
                     RTIRANLLDEVARPYVETARAKGVSERRLLFKYPVRIALIPYISVVGWSLPGLFSGET
                     IVAVVLSLPTTGPLLLEALQSQDMYLAGSFILMLSVLTVIGTLISDLLLAWADPRIRF
                     E"
BASE COUNT          603 a         1127 c         1130 g          533 t
ORIGIN      
        1 ccgctggcgc tggccgccgc tgaaggcatg gggatagcgg cgcatgtact ccgcgcgcag
       61 ccccacgtcc ttcatgaggg tctgaacgcg gtcctccaga tcccgcccgc gcgccatccc
      121 gttgacgaac agcggctcgc cgatgatctc ccgcaccgtc atgcgcggat tcaacgaccc
      181 gtagggatcc tggaagatca tctgcatgtg ccgccggatg cgtcgaagct catcccgcgc
      241 cagcgtggtg atctccaccg gagcgccgtc gacccgcatc cgcacgctcc cggcggtggg
      301 ctcgatggcg cgcaggatgg tgcgacccgc cgtggtcttc ccgcagccgc tctcccccac
      361 caggccgagg gtctctcccg cgcggatgga gaacgagaga ccgtccacgg cgcgcacgtg
      421 cccgaccacg cgtttcagga accccttctt gatcgggaag tgcttcctca gtcccgtgac
      481 ctccagcagg acgtcgtcac gcatgggcaa tcccctcctt cacgaacagg tgacatgcca
      541 cgcggtggcc gggagcgatc tccaggagcg gcgggatgct cttctcgcag gtcccgcgga
      601 ggaagcttcg gcaccgcggg tggaagggac agccccgcgg aacgttcaac gggtccggca
      661 ccaccccgcg gatcgactcg agcggcaccc cgctcttctt gccgaccttc gggatggagc
      721 gcaagagcgc gcgtgtgtac ggatgccgcg ggtcgtagaa caggtcgtcc gaggaggcct
      781 cctccacgag ccgacccagg tacatcacgg ctacctcctc ggccatctcg gcgatcacgc
      841 cgaggttgtg cgtgatcatg aggatggcca tgccgaactc gtccttgagg ttgcgcatga
      901 ggtccaggat ctgcgcctgg atggtcacgt ccagggcggt ggtcggctcg tcggcgatca
      961 ggagcttggg gcggcaggag agggccatgg cgatcatggc gcgctgccgc atcccgccgg
     1021 agagctggtg ggggtactcg tccacccgct ggcgtggctt ggagatcccg accttccaca
     1081 gcatctcgat ggccagctcc cgcgcctcct tcttgccggt gtgctggtgc agcaggatgg
     1141 cctccatgat ctggtttccg atcgtgtaga ccggggagag cgaggtcatc ggctcctgga
     1201 agatcatggc gatggtcttc ccgcggatgg cgcggatctc cctccccttg gggtccatgg
     1261 cggtaaggtc gatccctgcc tctccttcgc cgtgcaggag gatcttcccc tcgacgatcc
     1321 ggccacgggc gcccacgatc cgaaggatgg agcgcgccgt cacgcttttg ccgcagccgc
     1381 tctccccgac catccccagc gtgcggcccc ggtggaggtc gcagctcacg ccgtccaccg
     1441 cgtggacggt cccgtggtcc aggaagaagt atgtcttcag gttccggatc tccagcagcg
     1501 tttccatgcc ggcccctcta ccgcgcgtac gggtcggcgg catcccgcag cccgtccccc
     1561 aggaagttga aggccagcac ggtgaccacc acgaagaccc ccgggatcag cagccacggc
     1621 gcttgcgcca cggccttgat gttctgcgca tcctgcagga gcacgcccca gctgatggcc
     1681 ggcggccgca gacccagccc caggaaggag agcgcggtct ctgccaggat catcccagga
     1741 acggacatcg tgatggaagc gatgatgtgg ctgaagaagg acgggaccat gtgcttgagc
     1801 acgatgcgcg tctcacgcgc tccgatgagc cgcgcggaca tcacgaagtc ctcctcccgc
     1861 agggccagga acttgctgcg caccacgcgc gccagcccgg tccaccccac cagggagagg
     1921 atgatcgtga tcccgaagta cacgcggacc gccggccagt gcggcgggag cgccgcgctc
     1981 agccccatcc acagggggat ctgcggcatg cagatcacga actcgatgat ccgctgcacg
     2041 accgtgtcga agaacccgcc gtagtagccc gagaagcctc cgatcaggat cccgaggacg
     2101 aagctgagaa acacgccgac cagcccgatg gaggtcgaca gtcgaagccc gtagagcgtc
     2161 cgggacagca tgtcgcgacc cagccggtcg gtgcccagga ggaacatggt cccgtcgggg
     2221 tccgtgatcc cgaagagatg gaggtcggtc cggaccagac cccacatctt gtaggggttg
     2281 ccgtgcacga acaggtggat gggatacgcg tgctcgacgt ccggcttgta gacccgcagg
     2341 aggttctcgt cgtaaccgat cgtgtaccgg tagaccacgg ggcggagcgt gaagccggcc
     2401 ttcgtgacga acccgatcct ctgcggcgga gcgaagaagt agcgcttgtc gtagaccagg
     2461 ggatcatacg gcgcaaggaa ctcgcaggtg atcgacatca ggtagatcac gatcaggatg
     2521 atggcgctgg ccagcgccac cttgtggcgg cggaacttcc accacatgag ctgccactgg
     2581 gtggccacga agatgcgctc ttcgccgagg ggctcctcat cggcccctcc gccttcggcg
     2641 ccttccacgc ccggtgccgg ctcggagggc ggctcggccg ggtacggttg agcctcgtcg
     2701 cggcccgagg gtcgatcgtc catggctcct cctactcgaa gcggatgcgg ggatcggccc
     2761 aggccaggag cagatccgag atgagcgtcc caatgaccgt gagcacgctg agcatcagga
     2821 tgaagctgcc ggccaggtac atgtcctggc tctgcagcgc ctccaggagc agcggcccgg
     2881 tggtgggaag gctcagcacc accgccacga tggtctcccc cgagaacagc cccggcaggg
     2941 accacccgac cacgctgatg taggggatga gggcgatgcg gacggggtac ttgaacagca
     3001 gcctccgctc ggacaccccc ttggcccgcg ccgtctccac gtacggccgg gcgacctcgt
     3061 ccaggaggtt ggcccggatc gttcggatca ggcccgccgt gccggagagc ccgatgacca
     3121 gcgccggcat ccagaggtgg cgaaggagat ccagcgcctt ggcaaggctc cagggcgcct
     3181 ggatgaagtc cggagagaac agcccgccga cgctctggtt gaatacggag aacgcgatcc
     3241 acatgaatac cagcgcgatc aggaagctcg gggttgccag gccgatgaag ccaatcatcg
     3301 tgaaggtgta gtccccgagc gagtactggt gcgttgcgga gtagatccct atggggaacg
     3361 acacggccca ggtgaagatg agcgtgaaca ggg
//
LOCUS       DASRZS010000344         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_579967, whole genome shotgun sequence.
ACCESSION   DASRZS010000344 DASRZS010000000
VERSION     DASRZS010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579967"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /gene="fliM"
                     /locus_tag="VFH83_08375"
     CDS             complement(<1..747)
                     /gene="fliM"
                     /locus_tag="VFH83_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HET6486422.1"
                     /translation="MTEVLSQDEIDQLLTAISSGEVATEEVSAPADQRKIKIYDFKRP
                     DKFSKDQIRTVSIMHETFARQTTTALSSQLRSLAHVHVASVDQLTYEEFIRSIPNPTT
                     IAVINMDPLKGSAILEIDPAVTFAIIDRLFGGQGEGAKFTRELTDIEQSVIEGIIVRI
                     LGNLREAWTTVIDLRPRLGQIETNPQFAQIVPPSEMVVLVTLETKVGDVEGMMNFCIP
                     YLTIEPIIPKLSAQYVYSSMRRGTTTENLSL"
     gene            complement(766..1323)
                     /locus_tag="VFH83_08380"
     CDS             complement(766..1323)
                     /locus_tag="VFH83_08380"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HET6486423.1"
                     /translation="MSDEQQDLNLEDVGAGAEEAAGPRRGGLLSGMLLTILKWAAIGI
                     GVIIIVVTTTVITVRAVTKGATAASGLAAISPEYGPKAEPLSAYDGIDQIRGQTADDP
                     PATYLLQLSLGYLTTDAEFSVEIGKRKQQIQDLILKDISLKTADELSAPHWEELQAEL
                     LNTINAVMTTGKVRKVDFLSFIIVK"
     gene            complement(1414..1611)
                     /locus_tag="VFH83_08385"
     CDS             complement(1414..1611)
                     /locus_tag="VFH83_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HET6486424.1"
                     /translation="MVEVTRLDGKRYYVNPHQIEYVESNPDTTLIMLSGKRLVVREDY
                     QTLFGRIVEYRKLIGAFKNEE"
     gene            complement(1653..3041)
                     /gene="flgE"
                     /locus_tag="VFH83_08390"
     CDS             complement(1653..3041)
                     /gene="flgE"
                     /locus_tag="VFH83_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HET6486425.1"
                     /translation="MMRSLWAGVAGLQNHQTRMDVLGNNIANVNTNGFKKGRVTFQDM
                     LSQTLAGAARPTDTVGGVNPQQVGLGMQVAAIDTIHTQGALQSTGVMTDVALQGNGFF
                     VLNDGDKRFYTRNGAFGLDQDGRLVNPATGLRVQGWMAQTVGGQTFVNNASEMQDLVI
                     PVGSKDPAQATTDVWYASNLDKRTPEIPAGATAQTIQEGTRETNFDIYDSFGRVHKLQ
                     VNFSKVPGVANRWQAEVLVDPAAQPATNTTVQVGPTANNNNVFVLDFNNLGTLADVRD
                     AAGNLVSAGALQAAVSYDVPDVTLPAGQNAVRQTFNLNLGNTGSFVDAITQFGETSST
                     KAVKQNGYGMGYLETFKIDQSGTITGVYTNGSNRTIGQIGLASFTNPGGLEKAGDNTY
                     QASNNSGTANIGASGTAGKGKVIAGTLEMSNVDLAEQFTDMIVTERGFQANSRTITTS
                     DQMLQELLQLKR"
     gene            complement(3048..>3476)
                     /gene="flgD"
                     /locus_tag="VFH83_08395"
     CDS             complement(3048..>3476)
                     /gene="flgD"
                     /locus_tag="VFH83_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HET6486426.1"
                     /translation="TYNKTLNDGKPMSATLDKNDFLKILITQLTHQDPTQPMDDKEFV
                     AQMAQFSSLEQMTNMSQGLTKVADLVARSQAVSLLGNAVDLTDEQGNTVSGTVDAVTS
                     GDLPQLLVNGNYYDAANVQAVRRSLAAANAGNVPAAGAKE"
BASE COUNT          500 a         1089 c         1167 g          720 t
ORIGIN      
        1 gaggctcagg ttctccgtcg tggtgccgcg tcgcatcgag gagtacacgt actgcgcgga
       61 cagcttgggg atgatgggct cgatggtcag gtaggggatg cagaagttca tcatgccttc
      121 cacgtccccc accttcgttt ccagcgtcac gagcacgacc atctccgacg gcgggacgat
      181 ctgcgcgaac tgcgggttgg tctcgatctg tcccaggcgg gggcggaggt cgatgacggt
      241 ggtccacgcc tcgcgcaggt tgcccaggat gcgcacgatg atgccctcga tgaccgactg
      301 ctcgatgtcg gtgagctcgc gcgtgaactt cgctccctcc ccctgcccgc cgaagagccg
      361 gtcgatgatg gcgaaggtga ccgccgggtc gatctccaag atggcggatc ccttgagggg
      421 gtccatgttg atgacggcga tggtcgtggg gttggggatg gagcggatga actcctcgta
      481 ggtgagctgg tcgaccgatg cgacgtgcac gtgcgccagg ctgcgcagct gcgaggagag
      541 cgccgtcgtg gtctggcgcg cgaaagtctc gtgcatgatg gagacggtgc ggatctggtc
      601 cttggagaac ttgtcggggc gcttgaagtc atagatctta atcttgcgct ggtccgccgg
      661 cgcgctgact tcctccgtgg ccacctcgcc cgaggagatc gcggtgagaa gctggtctat
      721 ctcgtcctgc gaaaggactt ccgtcatccc cggttccccc gcggcctact tcacgatgat
      781 gaagctcagg aagtcgacct tcctgacctt gccggtggtc atgaccgcgt tgatcgtgtt
      841 caggagctcc gcctgcagct cctcccagtg cggcgccgag agctcgtcgg ccgtcttcag
      901 ggagatgtcc ttcaggatga ggtcctggat ctgctgcttg cgcttgccga tctcgacgct
      961 gaactccgcg tcggtcgtca ggtagccgag gctcagctgc agcaggtagg tggcgggagg
     1021 atcatcggcg gtctggcccc ggatctggtc gatgccgtca taggccgaca gcggctcggc
     1081 cttcgggccg tactcgggag agatggcggc gaggccgctc gctgccgtgg cgcccttcgt
     1141 gaccgcgcgc accgtgatga ccgtggtggt gacgacgatg atgatgacgc cgatgccgat
     1201 ggcagcccac ttgaggatcg tgaggagcat gccggacagc agaccgcccc ggcgaggccc
     1261 ggcggcttct tcggcgccgg cgccaacgtc ctccagattc aggtcctgct gttcgtcaga
     1321 catcccctcc cccccctctc catactatca caagaataca actcaacgca acccaggacc
     1381 accggaaaat ggcgttccgg tcggcgaatc ccttcattcc tcgttcttga aggcgccgat
     1441 cagcttgcgg tattccacga tacgcccaaa aagagtctgg tagtcctcgc ggactaccag
     1501 cctcttgccc gacagcatga tgagtgtcgt atcagggttg ctctcgacgt actcgatctg
     1561 gtgcgggttg acgtagtacc tcttcccgtc cagccgtgtc acctcgacca tcaagcctcc
     1621 tcagggcggt ggctcattcc tcatgaccgc ctctacctct tcagctgcag gagctcctgg
     1681 agcatctggt cggatgtcgt gatcgtgcgc gagttcgcct ggaagccgcg ctcggtcacg
     1741 atcatgtcgg tgaactgctc cgccaggtcg acgttggaca tctccagcgt gcccgcgatc
     1801 accttcccct tccccgccgt gcccgaggcg ccgatgttgg cggtgccgga gttgttggag
     1861 gcctggtagg tgttgtcgcc ggccttctcg aggccgccgg ggttcgtgaa ggaggcaagg
     1921 ccgatctgcc cgatggtcct gttggacccg ttggtgtaga cgccggtgat cgtcccggac
     1981 tggtcgatct tgaacgtctc caggtatccc atgccgtagc cgttctgctt gaccgccttc
     2041 gtggagctgg tctccccgaa ctgggtgatg gcatccacga aggatcccgt gttcccgagg
     2101 ttcaggttga aggtctggcg gaccgcgttc tgcccggccg gcagcgtcac gtccgggacg
     2161 tcgtaggaga cggcagcctg cagggccccc gcgctcacga ggttgcctgc cgcgtcacgc
     2221 acgtcggcca gcgtcccgag gttgttgaag tcgagcacga agacgttgtt gttgttcgcc
     2281 gtgggcccga cctggacagt cgtgttcgtg gcgggctggg ccgcggggtc cacgaggacc
     2341 tccgcctgcc agcggttggc cacgccgggg accttggaga agttgacctg gagcttgtgc
     2401 acccggccga acgaatcgta gatgtcgaag ttggtctccc gcgtgccttc ctggatggtc
     2461 tgggccgtgg caccagcggg gatctcgggc gtcctcttgt cgaggttgct ggcataccag
     2521 acgtcggtgg tcgcctgcgc ggggtccttg ctccccacgg ggatcacgag gtcctgcatc
     2581 tcggaggcgt tgttgacgaa ggtctggccg ccgacggtct gcgccatcca gccctgcacg
     2641 cgcaggcccg tggcggggtt caccagccgc ccatcctggt cgaggccgaa ggcgccgttg
     2701 cgcgtgtaga agcgcttgtc gccgtcgttg aggacgaaga agccgttgcc ctggagcgcc
     2761 acgtcggtca tcacgccggt cgactggagg gcgccctgcg tgtggatggt gtcgatggcc
     2821 gccacctgca tgccgaggcc gacctgctgg ggattcacgc cgcccacggt gtccgtgggc
     2881 cgcgcggcgc cggcgagggt ctgggacagc atgtcctgga aggtgacgcg gcccttcttg
     2941 aagccgttcg tgttgacgtt ggcgatgttg ttgcccagga cgtccatgcg cgtctggtgg
     3001 ttctgcaggc cggcaacacc ggcccagagt gatcgcatca tgcctgttta ctccttcgcg
     3061 ccggccgccg gcacattccc cgcgttcgcc gccgcgaggc ttctcctcac ggcctggacg
     3121 ttcgccgcgt catagtagtt cccgttgacg aggagctgcg gcaggtcccc gctggtgacg
     3181 gcgtccacgg tcccggagac cgtgttgccc tgctcgtcgg tgaggtccac cgcgttgccg
     3241 aggaggctga ccgcctggct gcgcgccacg aggtcggcca ccttcgtcag gccctggctc
     3301 atgttcgtca tctgctcgag gctggagaac tgcgccatct gcgcgacgaa ctccttgtcg
     3361 tccatgggct gcgtggggtc ctggtgcgtg agctgggtga tgaggatctt caggaagtcg
     3421 ttcttgtcca gcgtcgcgct catcggcttt ccgtcgttca gcgtcttgtt gtacgt
//
LOCUS       DASRZS010000345         7692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3270818, whole genome shotgun sequence.
ACCESSION   DASRZS010000345 DASRZS010000000
VERSION     DASRZS010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7692
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3270818"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFH83_08400"
     CDS             complement(<1..216)
                     /locus_tag="VFH83_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET6486427.1"
                     /translation="MLSQAEREAIDTQARHAAYPRAAVADALLVVQKSRGWVSDEGVR
                     DVAQALGLTAEEVDGVATFFELVFRKPV"
     gene            complement(392..2134)
                     /gene="nuoC"
                     /locus_tag="VFH83_08405"
     CDS             complement(392..2134)
                     /gene="nuoC"
                     /locus_tag="VFH83_08405"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HET6486428.1"
                     /translation="MSTNVIADLTTRFGEGAVAAQQTADAIPTAWIPAERLTDALTFL
                     RDAPGGSYPMLFDLSAIDERCRTHREGQPDSDFTVVYHLLSFERNQDVRIKVALPSDH
                     ASVRSVAGIYPNADWYEREVFDMFGVGFEGHPHLKRILMPPWWEGHPLRKEQPARATE
                     MGPFHMTQEDASRMLASLEFHPEDWGMRRDDGEHEYMFLNVGPQHPSAHGVLRLVAQM
                     DGEQIVDVVPDIGYHHRGAEKMGERQSWHTYIPYTDRIDYLGGVMNNFPYVLAVETLA
                     GIEVPDRAKVIRIMMAELFRVISHLVYYGTFAADVGQMSPVFWMFTDRERAFDIVEAV
                     TGGRMHPAWFRIGGVAQDLPQGWEKLVGDFIPYMRRRLKDYDAAVTRNGLVKARSRGI
                     GSVSLKEAMDWGASGPFLRACGFEWDFRKKRPYGGYENFQFDVPTGSHGDSYDRLMVH
                     VEEIRQSLRIIEQCLNGMPSGPYKSDHPLATPPRKERTMHDIETLIGHFLSVSWGPVI
                     PPGEAFAAVEATKGNHAYYLVSDGGTMSYRTRIRTPSFAHVQMVPLLSRGLTISDLVT
                     ILGSVDYVLADIDR"
     gene            complement(2152..2739)
                     /gene="nuoB"
                     /locus_tag="VFH83_08410"
     CDS             complement(2152..2739)
                     /gene="nuoB"
                     /locus_tag="VFH83_08410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HET6486429.1"
                     /translation="MKEAASSPLGSVAFARHADLLAWGRKYSMWPFNFGLSCCYVEMA
                     TSLTSRYDIARFGSEILRGSPREADLMVIAGTVFKKMAPLVLHLYQQMMEPRWVLCMG
                     SCANSGGMYDIYSVVQGVDSFMPVDVYVQGCPPSPDSFMRGLVLLAEAVATEQRPLSW
                     VVGPQGVVKAPKPSLRDAMGPERRAQTVLRSPDGL"
     gene            complement(2723..3133)
                     /gene="ndhC"
                     /locus_tag="VFH83_08415"
     CDS             complement(2723..3133)
                     /gene="ndhC"
                     /locus_tag="VFH83_08415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET6486430.1"
                     /translation="MSALLSLAVYLFAVVLLIGLLMAVTHVTGQRHFEPGTGIPYESG
                     IPPTGSARLRFSADFYLVAMFFVIFDISAVFLFAWAVAARELRWSGYVAILVFTLETV
                     VGLAYLWRMGAFDWGTRRLREHRERHGVGGEGGR"
     gene            complement(3210..4061)
                     /locus_tag="VFH83_08420"
     CDS             complement(3210..4061)
                     /locus_tag="VFH83_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET6486431.1"
                     /translation="MELARIFAPVASEMEEVDRRLRELLRQVARESQVRGSGPGIVSG
                     IVQYPFAVPGKRIRPALVLLTARMVGGPVDPESLVTVASAVEILHAASLVHDDIIDGA
                     DARRNQISLNKRFGNRVAVLAGDILYTHFFSLVTGLAGIDPGRRFALLDLFLDTTKAM
                     CAGEILAQEVGSARPLAYDDYREIASDKTAVLFSACCRSAAILCDTDGRSSALGEFGL
                     TFGLTFQMADDLVDGDHGLPPDYDLRARTLEHAARSRACLHSFPDGVYRESLSALIDY
                     VVDHAVS"
     gene            complement(4062..4844)
                     /locus_tag="VFH83_08425"
     CDS             complement(4062..4844)
                     /locus_tag="VFH83_08425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HET6486432.1"
                     /translation="MNTSYPSMGTITPSAHTAVVREIFTVSSARYDFLNHFLSLRRDV
                     GWRSCAVEQMRLDRANGSGRLLDVATGTGDLAIAAALRYPGVTVTGIDFARPMLEIGK
                     RKVRQLGLDGRIELLEGDALSLPFPDASFEVCSIAFGMRNIPDKRKALREMARVTVPG
                     GRVLILEMTFAPTWAFRPWYGFYLNRIMPTMARIFVRHGATYTYLADSIRHFPHPDRF
                     DQIMRESGFARVTHHGLTFGSAFLHIGRTQGAGGHSIRQGAR"
     gene            complement(4972..>7692)
                     /gene="ppdK"
                     /locus_tag="VFH83_08430"
     CDS             complement(4972..>7692)
                     /gene="ppdK"
                     /locus_tag="VFH83_08430"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET6486433.1"
                     /translation="VMSHLFGDSTNPLLVSVRSGARKSMPGMMETVLNVGLNERTAQG
                     LIASTGNPRFVYDAYRRLIQMYSDVVMEKAEDIEPAEGMGVRQQLERELERMKRAKGV
                     KQDTELNAEDLKALIRIYKTRVREILGKEFPEDPREQVWGAIRAVFKSWDGRRAKAYR
                     RIEGIPDEWGTAVTVQSMVFGNMGDDSGTGVAFTRNPATGENVFYGEWLPNAQGEDVV
                     AGIRTPYPINEAGKTEHTKDLPSLEKQFSKIYGNLHDIELRLEKHYHDMLDLEFTIEK
                     GKLWMLQCRVGKRNGPAAVRMAVEMVKEKLCGPTEAVTRITPAQLDELLHPILDPAKE
                     RETPILVKGLPAGPGGAVGQVVLTANDAVEWTKAGKKVILVREETNPEDVEGMRAAQG
                     ILTARGGMTSHAALVARGWGKCCIVGAGDLHVDVAAKQISIKGTTVREGDWLTLNGSK
                     GTAYVGALPLIKAAGENPDFVAFMAICDKVKKLGVRTNADTPQDAKRAREFGAEGIGL
                     FRFEHMFYGEGSDRPLFLLRRMIASKTEDERRKALQELFPFMKDDVKRTMEVMHGLPV
                     TFRFLDPPLHEFVPTRADERQRLANDLGISAEEFNKRADGLHESNPMMGHRGVRLGIS
                     YPEMTEVQSQAILEAAAELIQSGIDARPEIMIPVTSTMVEIVDQKKIIEAVYAKVLSK
                     FNLAKGKLNLVVGTMIEIPRACVTADKFASVCEFFSFGTNDLTQMGFGFSRDDIGGFL
                     PTYLEKKILPADPFQTIDQEGIGELIKMGITKGRSVKPTLKIGICGEHGGDPESVRFC
                     HRVGMSYVSCSPYRVPLAKLAAAQEAAAEMEAPKAAAKPAAKAAAKSSPVRAAQKVSK
                     AMPAARMPARKPAAKKPAAKKAAPRPVAKKPAARKTAAKKGASRKSARKR"
BASE COUNT         1268 a         2601 c         2464 g         1359 t
ORIGIN      
        1 aacgggcttg cggaagacga gctcgaagaa cgtggcgacg ccgtccacct cctccgccgt
       61 gaggcccagc gcctgggcca cgtcccggac cccttcgtcc gatacccagc cccggctctt
      121 ctgcaccacc agaagggcat ccgccacggc ggcgcggggg tacgcggcgt gtcgggcctg
      181 ggtatcgatc gcctcccgtt cggcttggct aagcattttc tctccactaa ccctttcgtt
      241 cggcgcggtc gcggagagcc gcgcctttcc gcggcggccc ccgatcggcg ccgccccttg
      301 ggcggggctt tacgccgccc cttgggcggg cctctacgcc cgggcggggc tctacgccgc
      361 cccttgggcg gggctctacg ccgccccttg gctatcgatc gatgtccgcc aggacgtagt
      421 ccacgcttcc aagaatcgtc accaggtccg atatggtcag gccgcggctc agcagcggca
      481 ccatctgcac gtgggcgaag gacggcgttc ggatcctggt acgatagctc atggtcccgc
      541 cgtcgctcac caggtaatag gcgtggttcc ccttggtggc ctccaccgcg gcgaaggcct
      601 ctcccggtgg aatgaccggc ccccagctca cgctcaggaa gtggccgatc agcgtctcga
      661 tgtcgtgcat cgtgcgctct ttgcgcggcg gggtggccag cggatgatcc gacttgtacg
      721 gccctgacgg catcccgttc aggcactgct cgatgatccg caggctctgg cggatctcct
      781 ccacgtggac catcagtcgg tcataggagt ctccgtggct gccggtgggg acgtcgaact
      841 ggaagttctc gtatccgccg tacggccgct tctttcggaa gtcccattcg aacccgcagg
      901 ctctcaggaa cggacccgag gcgccccagt ccatcgcctc cttcaggctg acgcttccga
      961 tgcctcggga ccgcgccttc accaggccgt ttcgcgtcac cgccgcgtcg tagtccttca
     1021 gccgacgccg catgtacggg atgaagtccc ccaccagctt ttcccaaccc tgggggagat
     1081 cctgggccac cccgccgatg cggaaccacg ctggatgcat cctcccgccg gtcaccgcct
     1141 ccacgatgtc gaaggcgcgc tcgcggtccg tgaacatcca gaacacggga gacatctgcc
     1201 ccacgtccgc ggcgaaggtc ccgtagtaga ccaggtggct gatcacgcgg aaaagctccg
     1261 ccatcatgat ccggatgacc ttggcccgat ccgggacctc gatccccgca agcgtctcca
     1321 cggccagcac gtaggggaag ttgttcatca cgccgcccag gtagtcgatg cggtccgtgt
     1381 acgggatgta cgtgtgccag ctctggcgct cccccatttt ctccgcgcca cggtggtggt
     1441 agccgatgtc cggcaccacg tccacgatct gctccccgtc catctgggcc accaggcgga
     1501 gcactccgtg ggccgagggg tgctgcgggc ccacgttcag gaacatgtac tcgtgctccc
     1561 cgtcgtccct tctcatgccc cagtcttccg gatggaactc cagggaggcg agcatccggg
     1621 aggcgtcctc ctgggtcata tggaaggggc ccatctccgt ggcccgagcc ggctgctcct
     1681 tgcggagggg atgtccttcc caccagggag gcatcaggat ccgcttcagg tgcgggtgcc
     1741 cttcgaagcc gacgccgaac atgtcgaaga cttcccgctc gtaccagtcg gcgttcgggt
     1801 agatgccggc cacgcttcgg accgaggcgt ggtccgaagg gagcgccacc ttgatccgca
     1861 cgtcctgatt ccgctcgaag gaaaggagat ggtagaccac cgtgaagtcg gagtccggct
     1921 ggccttcgcg gtgcgtccga cagcgctcgt cgatggcgct gaggtcgaag agcatgggat
     1981 aggagccccc gggagcgtcc cgcaggaacg taagcgcgtc cgtcaggcgt tcggcgggga
     2041 tccacgcggt agggatggcg tccgcggtct gttgggccgc cacggcgcct tcaccgaacc
     2101 gggtcgtcag atcggcaatc acattcgtgg acatgcgctg actcccgccg cctacagccc
     2161 gtccggagag cgcaggacgg tctgcgcccg gcgctcggga cccatggcat cccgcagaga
     2221 cggcttcggc gccttgacca cgccttgcgg gcctaccacc caggacagcg gccgctgctc
     2281 cgtggcaacg gcctccgcca ggaggacgag ccccctcatg aaggaatcgg ggctgggcgg
     2341 acatccctgg acgtacacgt ccacgggcat gaaagagtcc acgccctgga ccacgctgta
     2401 gatatcgtac attccgccgg agttggcgca ggagcccatg cagaggaccc accgcggctc
     2461 catcatctgc tggtacagat ggagcaccag gggcgccatc ttcttgaaca cggtcccggc
     2521 gatgaccatg aggtcagcct cccgcgggga gccccgcagg atctccgagc cgaaccgggc
     2581 gatgtcgtac cggctggtca gcgaggtagc catctccacg tagcagcagg agaggccgaa
     2641 gttgaacggc cacatcgaat acttgcggcc ccacgccaag agatccgcgt gccgggcgaa
     2701 cgcgacgctc cccagcggcg agctagcggc ctccttcacc acccaccccg tgcctttccc
     2761 ggtgctcccg gagcctgcgc gttccccaat cgaatgcgcc catgcgccag aggtaagcca
     2821 ggccgaccac cgtctccaag gtgaaaacca ggatcgcgac gtatcccgac cagcgaagct
     2881 cccgggccgc cacggcccac gcgaagagga acaccgcgga gatgtcgaag atcacgaaga
     2941 acatggcaac caggtagaag tccgccgaga agcgcagacg ggcggacccc gtgggcggga
     3001 tcccggactc gtatgggatc cccgtgccgg gctcgaagtg ccgctggccc gtgacgtggg
     3061 tcaccgccat cagcaaaccg atcaggagca ctacggcgaa caggtagaca gccagggaaa
     3121 gcagcgcgct cattctttct cctgcaagga tccggttgga gcacgggcag acaggcttgc
     3181 gctcaaaata gctactccgc gggcggtagt caagaaaccg cgtgatcgac cacgtaatcg
     3241 atcagggccg acaacgactc gcggtagact ccgtccggga atgaatgaag gcaagcccgg
     3301 gagcgcgcgg cgtgctccag ggtgcgggct cgcaggtcgt aatccggggg aagtccgtgg
     3361 tccccgtcca ccaggtcatc ggccatctga aaggtgaggc caaacgtcag cccgaactcg
     3421 ccgagggcgc tggaccggcc gtccgtatca cagaggatgg cggcggaccg gcagcacgcg
     3481 gagaagagca ccgcggtctt gtccgatgcg atctcgcggt agtcgtcata tgccagcggc
     3541 cgggcggagc cgacttcctg ggccaggatc tcacccgcgc acatcgcctt ggtggtgtcc
     3601 agaaagaggt ccaggagagc gaatcgccgg cccgggtcga tgccggccag acccgtgacc
     3661 agcgagaaga agtgggtgta caggatgtcg ccggccagca cggccacccg attgccgaaa
     3721 cgcttgttga ggctgatctg gttgcggcgg gcatcagcgc cgtcgatgat gtcgtcatgt
     3781 acgagagacg cggcgtggag gatctccacc gcggaagcca cggtgaccag ggactccggg
     3841 tccaccgggc cgccgaccat ccgagcggtc agcagcacca gggccggtcg gattcgcttt
     3901 ccgggaacgg cgaaggggta ctgcacgatt cccgagacga tccccgggcc gctgcctcgg
     3961 acctggctct cccgcgctac ctggcgcagg agctcgcgga gcctgcgatc cacctcttcc
     4021 atttccgatg caacgggcgc gaatatccgt gccagttcca ttcacctagc tccctgtcgg
     4081 attgagtggc cgccggcccc ctgggtccgc ccaatatgca ggaaggcgct tccgaaggtc
     4141 aacccgtgat gggtcacccg cgcgaagccg ctctcccgca tgatctggtc gaaacggtcg
     4201 ggatgcggga agtggcggat ggagtccgcc aggtacgtat aggtggcccc gtggcgcacg
     4261 aagatccgcg ccatcgtcgg catgatccgg ttcaggtaga agccgtacca cgggcgaaag
     4321 gcccaggtgg gggcgaaggt catctccagg atcaggaccc ggccgcccgg cacggtcacc
     4381 cgtgccatct cccgaagcgc ctttcgcttg tccgggatgt tgcgcatacc gaacgcgatc
     4441 gagcatacct cgaagctcgc gtccgggaag ggcagggaca aggcatctcc ttccaggagc
     4501 tcgatcctgc cgtccagacc gagctgtcgg accttgcgct tgccgatctc cagcatgggc
     4561 cgggcgaagt cgatccccgt gaccgtcacc ccggggtatc ggagcgccgc ggcgatagcc
     4621 aggtcccccg tccccgtagc cacgtccagg agccgccctg acccgttcgc ccggtccaga
     4681 cgcatctgct ccaccgcgca ggagcgccaa cccacgtccc gccgcaggct caggaagtgg
     4741 ttcagaaagt cgtagcgggc agaggacacg gtgaagatct ctcggaccac cgcagtgtgc
     4801 gcggacgggg tgatggtgcc catggaaggg tatgatgtgt tcatcccagt ggctagagca
     4861 taagcagtac ggcgggcaaa agggaagggg gactcggcga ggcggatctc cagaaactga
     4921 aacggccctg accgtcagag tcggccaggg ccgtggtttg gcgtccgccc gtcagcgctt
     4981 gcgtgccgac ttcctgctcg cgcccttctt tgccgccgtc ttcctggcgg caggcttttt
     5041 cgccaccggc cgcggggcgg ccttcttcgc cgccggcttc ttcgcggccg gcttccgagc
     5101 aggcatacgg gcggccggca tggccttgct caccttctgg gcggcgcgga ccggcgagct
     5161 tttcgcggcc gccttcgctg ccggcttggc cgccgccttt ggcgcttcca tctccgcggc
     5221 ggcttcctgc gcggcggcca gcttggccag cggcacgcgg tacggcgagc aggagacgta
     5281 gctcataccg acgcggtggc agaaccggac ggattcgggg tcaccaccgt gctcgccgca
     5341 gatgccgatc ttgagggtcg gcttcacgga ccggcccttg gtgatgccca tcttgatcag
     5401 ctcgccaatg ccttcctggt cgatggtctg gaacgggtcc gcggggagga tcttcttctc
     5461 cagatacgta ggcaggaacc cgccgatgtc gtctcgagag aacccgaagc ccatctgggt
     5521 caggtcgttg gtcccgaagg agaagaactc gcatacgctg gcgaacttgt ccgccgtgac
     5581 gcacgcgcgc gggatctcga tcatcgtccc gaccaccagg ttcagcttgc ccttggccag
     5641 gttgaacttg gagaggacct tggcgtacac ggcctcgatg atcttcttct ggtccacgat
     5701 ctccaccatg gtggaggtga ccgggatcat gatctcgggc cgcgcgtcga tgccggactg
     5761 gatcagctcc gctgccgctt ccaggatggc ctggctctgc acctcggtca tctcgggata
     5821 ggagatgccc agtcgcacgc cccggtgacc catcatgggg tttgactcgt gcaggccgtc
     5881 cgcgcgcttg ttgaactctt cggcggagat ccccaggtcg ttggccaggc gctgccgttc
     5941 gtccgcccgg gtcggcacga actcgtgcag cggaggatcg aggaaccgga aggtgaccgg
     6001 aagcccgtgc atgacctcca tggtcctctt cacgtcgtcc ttcatgaatg ggaagagctc
     6061 ctggagggcc ttgcggcgtt catcctcggt cttggaggcg atcatgcggc gcaggaggaa
     6121 cagcggtcga tcggacccct ccccgtagaa catgtgctcg aaccggaaga gcccgatgcc
     6181 ctctgccccg aactccctcg ccctcttggc gtcctgggga gtgtcggcgt tggtgcggac
     6241 gcccagcttc ttcaccttgt cgcatatggc catgaaggcg acgaagtcgg ggttctcacc
     6301 ggccgccttg atgagcggca gggcgcccac gtaggccgtc cccttgctgc cgttgagggt
     6361 cagccagtcg ccttcccgga ccgtggtgcc cttgatggaa atctgctttg ccgcgacgtc
     6421 cacgtggagg tccccggcgc cgacgatgca gcacttgccc cagccgcggg ccaccagggc
     6481 cgcgtgggag gtcatgccgc ctcgggccgt aaggatgccc tgcgcggcgc gcatcccttc
     6541 gacgtcctcg ggattggttt cttcccggac caggatgacc ttcttgccgg ccttggtcca
     6601 ttccaccgcg tcgttggcgg tgagcaccac ctgtcccacg gcgccgccgg gtccggccgg
     6661 aagacctttc accagaatcg gggtctcacg ctccttggcg ggatccagaa taggatgcag
     6721 gagctcgtcg agctgggccg gagtgatgcg ggtcaccgct tcggtcggtc cgcacagctt
     6781 ctccttcacc atctccacgg ccatccgcac cgctgccggg ccgtttctct tgccgacgcg
     6841 gcactggagc atccagagct tgcctttttc aatggtaaac tccaggtcca gcatgtcgtg
     6901 gtagtgcttc tcgagcctga gctcgatgtc gtgcaggttt ccgtagatct tcgagaactg
     6961 cttctccagc gacggcagat ccttggtgtg ctcggtcttg cccgcctcgt tgatgggata
     7021 cggcgtgcgg atccccgcca ccacgtcctc accctgggcg ttgggcagcc actcaccata
     7081 gaagacattt tctccggtcg ccgggttgcg ggtgaacgcc acgccggtgc ccgagtcatc
     7141 ccccatgttc ccaaacacca tggattgcac cgtgaccgcg gtgccccact cgtcggggat
     7201 cccttcgatg cgccggtacg ccttggcgcg cctcccgtcc cagctcttga acacggcccg
     7261 gatggcgccc cagacctgct ctcggggatc ttcggggaac tccttgccca ggatctcccg
     7321 aaccctggtc ttgtaaatgc ggatgagcgc cttcaggtcc tcggcgttca gctccgtatc
     7381 ctgctttact cccttggcgc gcttcatgcg ctccagctct cgttcgagct gctggcggac
     7441 gcccattccc tccgcgggct cgatgtcctc cgccttctcc atgaccacgt cggagtacat
     7501 ctggatcagt cggcgatagg catcatagac gaaccggggg tttccggtgg aagcgatcag
     7561 gccctgggcc gttctctcgt tcagacccac gttcaggacg gtctccatca tgcccggcat
     7621 ggacttgcgg gctcccgacc ggaccgaaac cagaagggga ttggtggaat cgccgaacag
     7681 gtggctcatc ac
//
LOCUS       DASRZS010000346         4097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1348946, whole genome shotgun sequence.
ACCESSION   DASRZS010000346 DASRZS010000000
VERSION     DASRZS010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348946"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..314)
                     /locus_tag="VFH83_08435"
     CDS             complement(27..314)
                     /locus_tag="VFH83_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET6486434.1"
                     /translation="MNKKLYVGGLSYSVTDEQLQQLFAAHGTVESAKVILERDTNRSR
                     GFGFVEMSTQEEAEKAINALNGTQHEGRSLTVNFSKPREERPARGNYRDRY"
     gene            664..1401
                     /locus_tag="VFH83_08440"
     CDS             664..1401
                     /locus_tag="VFH83_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6486435.1"
                     /translation="MSTEHTPTDHPAPQQNRLLAVLPRPEAERLLPHLEPVSLPLGKA
                     LYESGGRLDHVYFPTTAIVSLLYELENGASAEIAVVGNEGTVGIALFMGGGTMPNRAV
                     VQSAGGAYRLQGTLLQQEFNRGGALQHLLLRYTLALLAQMAQTAVCNRHHTVDQQLCR
                     WLLLSLDRLPANELSMTQELIANMLGVRREGVTEAAGKLQSAGLIRYNRGRITVLNRP
                     GLEQRVCECYEVVRQEFIRLLPDTPES"
     gene            complement(2149..3348)
                     /locus_tag="VFH83_08445"
     CDS             complement(2149..3348)
                     /locus_tag="VFH83_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014764190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HET6486436.1"
                     /translation="MAKIVCVLYADPVDGYPRTYPRDDVPRLERYPNGQTLPTPKSID
                     FKPGQLLGSVSGELGLRRFLESMGHTYVVTADKDGPSSVLERELPEAEVVISQPFWPA
                     YMTAERIAKAPKLKLIITAGIGSDHTDLQAAIEHRLTVAEVTFSNSISVSEHVVMMIL
                     ALVRNYIPSYGWVVKGGWNIADCVARSYDLEGMTVGTVGAGRIGLAVLRRLKPFDVKL
                     HYTDRYRLDPKIEAELGLTYHPNAESLVKVCDVVTINTPLHPETEGLFNDALIARMKR
                     GAYLVNTARGKICDRDAIARALEKGQLAGYAGDVWFPQPAPKDHPWRTMPHHGMTPHI
                     SGSSLSAQARYAAGTREILECYFAGKPIREEYLIVDKGRLAGAGAHSYSAGNATRGSE
                     EATRFKG"
     gene            complement(3500..>4097)
                     /locus_tag="VFH83_08450"
     CDS             complement(3500..>4097)
                     /locus_tag="VFH83_08450"
                     /inference="COORDINATES: protein motif:HMM:NF020354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HET6486437.1"
                     /translation="DTAVEEAVLEHYRRVRWEGFHTENELWNGLFGLAFWDIVFMPVP
                     GAFHNRFQRGPRDLFSSGFRHRRREAIERRLAEIRESPAWSEQLRGTYDRCQGIANAL
                     VAWDRLPSGLLDRALTRIPPEHLALVFDRMAANLGVHRAGFPDLVLFPPLDAEPGGAY
                     HLVEVKGPGDQLRPNQERWLRLFAEHGIPARVVRVERE"
BASE COUNT          686 a         1352 c         1301 g          758 t
ORIGIN      
        1 aaccgccccc gtcccttgtt tcttttctag tagcgatccc ggtagttgcc gcgagccggg
       61 cgctcttcac gaggcttgga gaagttcacg gtcaggctcc tgccttcgtg ctgcgtgccg
      121 ttgagggcgt tgatcgcctt ctccgcttcc tcctgcgtgg acatttcgac aaatccgaaa
      181 ccacgcgagc ggttcgtgtc ccgttccagg atgacctttg cggattccac ggtcccgtgg
      241 gccgcgaaca gctgctgcag ctgctcatcc gtgaccgaat acgaaagacc gccaacgtac
      301 agtttcttgt tcatgcgctc ctccttggag tgtggtgctt catcgccttc atctcgtacc
      361 tgctaaggag cgaacatgcc agaatcttgc tgggataccg ctgtccgggt cggtgttcct
      421 tcggggtagg actgcaaagg tgcaattcac caggcttctt tttgcttctg tccgactctg
      481 cgaagccgtg cttgagcgga cgagaacagg ctacagggtt cggcgcaccc tgtcaagagc
      541 cttcggtcgc gcgccgcggg gcctccgtgt tgcgttgtac gattctccct gacttgacag
      601 gcaccccgcc cgagcctcga aaatccgatc gtccgattcg gacaagcaaa cgggaggttg
      661 cctatgtcca ccgagcacac ccctacggat caccccgccc cgcagcaaaa ccgtctcctc
      721 gcggttctcc cccggcccga agccgagcgg ctgcttcccc acctggagcc ggtatccctg
      781 cccctcggga aggcgctcta cgagtccggg ggtcggttgg accacgtcta tttccccacg
      841 acggccatcg tgtcgctgtt gtatgagctg gagaacggag cttcggcgga gattgccgtt
      901 gtcggcaacg agggcaccgt cgggatcgcc ctgttcatgg ggggcggcac catgccaaac
      961 cgggccgtcg tgcagagtgc cggtggcgcg tatcgactcc aggggacgtt gctccagcag
     1021 gagttcaacc ggggaggagc gctgcagcac ctgctgctgc gctacaccct ggccctgctc
     1081 gctcagatgg ctcagactgc cgtctgcaac cgccaccaca ccgtagacca gcaactctgt
     1141 cggtggctcc tgctcagcct ggaccggtta ccggcgaacg agctgagcat gacccaggag
     1201 ttgatcgcca acatgctcgg agtccgacgc gaaggcgtga cggaggctgc tggaaagctc
     1261 cagagtgccg ggctgatccg gtacaaccga gggcgcatca ccgtgctgaa tcgaccgggt
     1321 ctggaacagc gggtgtgcga gtgctacgag gttgtccgtc aggagttcat tcgcctgctc
     1381 cccgacactc cggagagctg acgcccggat ccgctggccg gaacaaaggg cgcccgatcc
     1441 caaagctcca gatcctggct tctccttcgg ctgcactcgt cacacaaggg aaccatgtac
     1501 caggtcgtgt cagaagaagg gccgtccagt tgaaccaggc ggacggccgt tggtctgcgt
     1561 atgcatccta cggaaaaaca caggtaggct tgatggcgac tgaccctttc ccaaagggca
     1621 agtccacttt ctccatcgcc cgcgccggcc gaccccatgc tcaagttcct taccttcatg
     1681 gttcgtgccc ccctggggac ttacgctctc ctccatcaag accgcgacac ggggattgcg
     1741 ccttcgtccc cggggctgcg tagtctaccc tcgccaaccg gcgtcttccg tgcggcagcg
     1801 cacattgcgg gacggaccgc agcgaacgcc ctgccggcgg gaaggcgctt gaccgggccc
     1861 gttgcccgta ccatcccaga accgtgaccg ctccatccac tcgggatttt gcgtccgcgg
     1921 cggacgcggc cgtcatcgag aagacgcggg aagccttgag cgcgcgtggg gtcatggtaa
     1981 cggtggtcga tacgcggcca gaggcgctcg acagactgaa ggcgttgatt cccgcgggag
     2041 cctcggtgaa ctccggggcg tccgtgacgc tgcttcctgg gcagcgccca cgccggccgg
     2101 accgtgaacc tcctcctcgt caaggaaccc gtcggggatt gagatccctc atcccttgaa
     2161 gcgcgtggcc tcctcggatc cgcgggtggc gttccccgcg ctgtaggagt gcgcgccggc
     2221 gcccgccagt ctgcccttgt ccacgatcag gtactcctcg cgaatcggct tcccggcaaa
     2281 gtagcactcc aggatctcac gggtacccgc ggcgtatcgg gcttgcgcgg agagggagga
     2341 gcccgagata tggggcgtca tcccgtggtg cggcatggtc cgccaggggt gatccttcgg
     2401 cgcgggctgg gggaaccaca cgtcgccggc ataccctgcc agctgaccct tctcgagcgc
     2461 ccgggctatg gcgtcgcggt cgcagatctt cccgcgagcg gtattcacca ggtaggcgcc
     2521 gcgcttcatc ctggcgatca acgcgtcgtt gaacaggccc tcggtctccg ggtgcagcgg
     2581 cgtgttgatc gtcaccacgt cgcatacctt caccagggac tccgcgttcg gatggtacgt
     2641 gaggccgagc tccgcctcga tcttcgggtc caggcggtag cggtccgtgt agtggagctt
     2701 cacgtcgaac ggcttgaggc gccgaagcac ggccaacccg atccgacccg cacccaccgt
     2761 tcccacggtc atgccttcca ggtcgtacga gcgggcaacg caatccgcga tgttccagcc
     2821 gcccttcacc acccagccgt acgatggtat gtagttgcgg accagcgcca ggatcatcat
     2881 gaccacgtgc tccgatacgc tgatgctgtt ggagaaggtg acctcggcca ccgtaaggcg
     2941 atgctcgatg gcggcctgca ggtccgtgtg gtccgagccg atccccgcgg tgatgatcag
     3001 cttcagcttc ggagccttcg cgatccgctc ggcagtcatg taggccggcc agaacggttg
     3061 ggagatgacc acttcggcct ccggaagctc ccgttccagc acggagctcg ggccatcctt
     3121 gtccgccgtg accacgtagg tatggcccat cgattccagg aatcgccgta ggcccagctc
     3181 gcccgaaacg cttcccagga gctggcccgg tttgaaatcg atcgacttcg gggtgggcag
     3241 cgtctgcccg ttcgggtagc gctccagccg tgggacgtcg tcccggggat aggttctggg
     3301 atacccgtct acgggatccg catagaggac acagacgatc ttcgccatac ggcggctctc
     3361 cttgtacgtt ggatttcttt tacggttggt tggcgaaggg tagggttgcc gtgggacagg
     3421 tgggggctct cgatctccaa cctcacggcc atggtcggaa ggtaccgaaa ccggttggga
     3481 cggtcaaaag aggcgacgct cactcccgct cgacgcgcac cacgcgcgcc gggatcccgt
     3541 gctcggcgaa caggcggagc cagcgctcct ggttcgggcg gagctggtct cccggcccct
     3601 tcacctccac caggtgatag gcgccgcccg gctcggcgtc cagcggcgga aacagcacga
     3661 ggtcgggaaa gcccgcccgg tgaacgccga ggttcgccgc cattcgatcg aagaccaggg
     3721 ccaggtgctc cggcgggatg cgcgtcagcg cccggtcgag caggccggat gggaggcggt
     3781 cccaggccac cagcgcgttg gcgattccct ggcagcggtc gtaggtgcct cgcagttgct
     3841 cggaccatgc cggggactcg cggatctcgg ccagacgacg ttcgattgcc tcgcgccggc
     3901 gatggcggaa cccggatgag aacaggtcgc ggggaccgcg ctggaaccga ttgtggaacg
     3961 cgccaggcac gggcatgaac acgatgtccc agaaggcaag gccgaagagc ccgttccaca
     4021 gctcgttctc ggtgtgaaac ccctcccagc ggacccgccg gtaatgctcg agcaccgcct
     4081 cctctacggc ggtatcg
//
LOCUS       DASRZS010000347         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5192827, whole genome shotgun sequence.
ACCESSION   DASRZS010000347 DASRZS010000000
VERSION     DASRZS010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5192827"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VFH83_08455"
     CDS             complement(<1..1212)
                     /locus_tag="VFH83_08455"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET6486438.1"
                     /translation="MKPVARKTLASLIAEQIRREIVHGNLKASQRLPSEQELSAELGV
                     GRPTLREALRILEGQGWVQIQFSTGVFVSDPENLADRMSTYYGKEGILDLLESEVSQL
                     VQEGREVPESARAELSALKENGKPREIEELIERLQQLPIASSFPYVEPTTLADIRASR
                     APRADGETASARGLFALACARERIHGGLLGRCVGSLLGTPVNGWTSGAIEQYLRAVGR
                     YPLTGLMPYAPKEAARGEYSFRGTEPSDSPLRQKALLRGVAPEYAILNDTVLSLKVMQ
                     LHGWDFSSDDVGGEWLSSLPYRATLTAERQAYGNLVRGLSPPATATILNPFREWIGGT
                     VRADLYGYVCPGDPAAAAMLAYRDATVSHTKNGVYGAMFVAAAIASAFGAGTAEEVVA
                     AGMGEIPQRSRM"
     gene            complement(1788..>2050)
                     /locus_tag="VFH83_08460"
     CDS             complement(1788..>2050)
                     /locus_tag="VFH83_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486439.1"
                     /translation="TNVFTTAATATASSTSTQTTVTNSSTVWNGRMYWMLRWTPMSAV
                     TLDLFGQTIMNALNFSIFGAGGGFNPNTFISSLGLSATFHLQ"
BASE COUNT          361 a          632 c          701 g          356 t
ORIGIN      
        1 catccgcgag cgctggggga tctctcccat gcccgcggcc accacctctt cggcggtccc
       61 ggcgccgaac gcggaagcaa tggccgccgc cacgaacatg gcgccgtaca cgccgttctt
      121 ggtatgggac accgtggcgt cccggtaggc cagcattgcc gcggccgccg ggtcaccggg
      181 acacacgtag ccatagaggt ccgcgcggac ggttccgccg atccattccc gaaagggatt
      241 gaggatggtg gcggtggccg gcggcgagag cccccgtacc aggtttccgt aggcctgccg
      301 ttccgcggtt agggtggcgc ggtacgggag ggaagagagc cattcccctc ccacgtcgtc
      361 ggaggagaag tcccagccgt ggagctgcat gaccttcagg ctgaggaccg tgtcgttcag
      421 gatcgcgtat tcaggagcca cgccgcgcag gagcgctttc tggcgaaggg gggaatccga
      481 aggctcggtt ccccggaagg agtactcccc ccgagcggct tccttcggcg cgtagggcat
      541 gagtccggtg agcgggtagc gacccaccgc cctcaggtac tgctcgatgg cgccggaggt
      601 ccacccgttg accggagtgc ccaggaggct gccaacacat cgacccagga gcccgccgtg
      661 aatgcgttcc cgcgcgcagg caagggcgaa gagtcctcgg gccgaggcgg tctcgccgtc
      721 cgcccgcgga gcgcgggagg ctcggatgtc ggccagggtg gtcggttcga cgtaggggaa
      781 ggaggaagcg atcgggagtt gctgcagtcg ctcaatgagc tcctcgatct ctctgggctt
      841 gccgttctcc ttgagggcag agagctcggc gcgcgccgac tcggggacct cccggccctc
      901 ctggacgagc tgggacacct cggattccag caggtcgagg atcccttcct tgccgtagta
      961 cgtggacatc cggtccgcaa ggttttccgg atcggagacg aacacgccgg tcgagaactg
     1021 gatttggacc catccctgac cctcgaggat ccgaagtgcc tcccgaaggg tcggtcgtcc
     1081 gacgcccagc tccgcgctga gctcctgttc gctggggagc cgctgggaag ccttcagatt
     1141 gccgtgaacg atctctcgcc ttatttgctc ggcgatcagg gaggcaaggg tcttccgtgc
     1201 aacgggcttc atgggtctcc ttgcagaccc cggcgatcaa cggacctcgc cggttcagaa
     1261 ccgcagggtg cgctcaggca acgcgccgct ggccgcgcat ctggacagcg caccccgggc
     1321 cacctgcccc ggcccgcgcc ccaggctggg cgcccccggc tattgtaaga ttgtcttaca
     1381 atgccgcgat tctagatccc aaggagaggg atgtcaagtt cagggtgatt gccgcgaggt
     1441 gctcgatgcc ttcgttgtca gagcgggagg ccttctcaac aaacgggtcc ggagtcgggc
     1501 cttggcatcc agggctgcgc gagacatccg ggctggcgtg gaccgaacga tctcctcgta
     1561 gtgcttgagt cttgcggtag cgtgcagcaa caggtacgga tccatgacaa tctccatagt
     1621 cacaaccggc atggcgaaag aaaccgaacg cgtgttcgag ggttcaacaa cggggaccgt
     1681 tggagttgtc accgcgacga cgaggaatcg cgtcttcggc tcaaccccgt ggagaagaaa
     1741 ggggctgtcc atggcggaca gccccaaaac ggttggctga gcttggccta ctggaggtgg
     1801 aacgtcgcgc tcaaaccgag ggagctgatg aaggtgttcg ggttgaagcc gccgccagcg
     1861 ccgaagatgc tgaagttcag ggcgttcatg atggtctggc cgaacagatc caacgtgacc
     1921 gcggacatcg gggtccagcg cagcatccag tacatccggc cgttccacac ggtactggag
     1981 ttggtaaccg tagtctgggt cgaggttgag gaggccgttg ccgtggccgc ggtcgtgaac
     2041 acgttcgtac
//
LOCUS       DASRZS010000348         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_580546, whole genome shotgun sequence.
ACCESSION   DASRZS010000348 DASRZS010000000
VERSION     DASRZS010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580546"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VFH83_08465"
     CDS             <1..940
                     /locus_tag="VFH83_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486440.1"
                     /translation="RFPALLNAPPQSIHLRGADGRIRAPFIYAEAQALDADTFQYVFT
                     EDKTRVYPVRLFVRAEKYRFLGILRTDVHLFGTGDPTVPIFLFGSDTLGRDLFSRIVL
                     GTRISLTIGLVGVLIGFVLGLLIGGVSGYFGGTVDAIIQRCIDFLISMPQLPLWMALS
                     AALPRNWPVVKTYFAITIVLSIIGWCGLARVVRGKLLALREEDFAMAARAGGASEWRI
                     ITRHLLPSFFSYLVVNITLAIPGMIIGETSLSFLGLGMRPPAVSWGVLLQDAQNIIVV
                     AQRPWQLIPCIFVIVTVLMFNFLGDGLRDAADPYAR"
     gene            952..1956
                     /locus_tag="VFH83_08470"
     CDS             952..1956
                     /locus_tag="VFH83_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486441.1"
                     /translation="MADSDVLLSVVDLRTHFTLHEGTVKAVDGVSFDVKRKQTLGVIG
                     ESGSGKSVTAQSVLRIVPDPGRIVTGRILFHAHNGDALDLAALDPMGQEIRRIRGKDV
                     AMVFQEPMTSFGPLSTIGHQITETILLHGMASSLHEAREQTVELLDKVGIARPRVIVD
                     CYPHQLSGGMRQRAMIAMALSCRPLLLIADEPTTALDVTIQAQILDLMQKMQDEFAMS
                     ILYITHNLAVVSEIADEVVVMYFGKVMERGSTRELFTNPLHPYTQALWRSIPTIDGPR
                     TRLNTIAGMMPKPYEVPRGCVFFGRCPVGVEGVCNAAPPPVVEVAPGHAVHCVKAAGK
                     "
     gene            1953..>2070
                     /locus_tag="VFH83_08475"
     CDS             1953..>2070
                     /locus_tag="VFH83_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009795208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6486442.1"
                     /translation="MNGEPAVLLDVNGLKKYFPVEGGALGRGRGWVRAVDDVS"
BASE COUNT          320 a          717 c          661 g          372 t
ORIGIN      
        1 ccggttcccc gcgctcctga acgcgccgcc ccagagcatc cacctccggg gcgccgacgg
       61 cagaatccgc gcgccgttca tctacgcgga ggcacaggcc ctggacgcgg acaccttcca
      121 gtacgtgttt acggaagaca agaccagggt ctatccggtt cggctcttcg tccgcgcgga
      181 gaagtaccgc ttcctcggga tcctccgcac cgacgtgcac ctcttcggga ccggggaccc
      241 gaccgtgccg atcttccttt tcggctcgga caccctgggt cgggacctct tctcgcggat
      301 cgtcctgggc acccgcatct ccctgaccat cgggctggtc ggagtgctca tcgggttcgt
      361 cctgggcctc ctcatcggcg gagtctcggg ctacttcggc ggcaccgtgg atgccatcat
      421 tcagcgctgc atcgacttcc tgatctcgat gccgcagcta ccgctgtgga tggcgctctc
      481 cgccgccctc cctcggaact ggccggtggt caagacctac ttcgccatca ccattgtgct
      541 ctccatcatc ggatggtgcg gtctcgcccg ggtggtgcgg gggaagctcc ttgccctgcg
      601 ggaggaagac ttcgccatgg cggcgcgcgc cggcggcgcc tcggagtggc gcatcatcac
      661 ccgccacctc ctgccctcgt tcttcagtta cctggtggtg aacatcaccc tggccatccc
      721 cggcatgatc atcggggaga ccagtctcag cttcctgggg ctggggatgc gccctccggc
      781 ggtgagctgg ggcgtgctgc tgcaggacgc tcagaacatc atcgtggtcg ctcagcggcc
      841 gtggcagctc atcccctgca tcttcgtcat cgtcaccgtg ctcatgttca acttcctggg
      901 ggacgggctg cgcgacgccg cggacccgta cgcccgttga ggaggacgac gatggcggat
      961 tcggacgtcc ttctcagcgt cgtggacctc aggacgcatt tcaccctcca cgaggggacg
     1021 gtcaaggccg tggacggcgt gagcttcgac gtgaagcgaa agcagacgct gggggtcatc
     1081 ggggaaagcg gttcgggcaa gtccgtgacc gcgcagtccg tcctgcggat cgtgccggac
     1141 ccgggtcgga tcgtgaccgg tcggatcctc ttccatgccc acaacggcga cgctctggac
     1201 cttgccgccc tggaccccat gggccaggag atccgcagga tccgaggcaa ggacgtggca
     1261 atggtgttcc aggagcccat gacgtccttc ggacccctct ccaccatcgg ccaccagatc
     1321 accgagacca tcctgctgca cggcatggcg tcctccctcc acgaggcgcg ggagcagacc
     1381 gtggagcttc tggacaaggt gggcatcgcc cggccccggg tcatcgtaga ctgctacccg
     1441 caccagctca gcgggggcat gcgccagcgg gccatgatcg ccatggccct ctcgtgtcgt
     1501 ccgctgctgc tcatcgcgga tgagcccacc accgctctgg acgtcaccat ccaggcgcag
     1561 atcctggacc tgatgcagaa gatgcaggac gagttcgcca tgtccatcct ctacatcacc
     1621 cacaacctcg cggtggtctc cgagatcgcc gacgaggtgg tggtgatgta cttcgggaag
     1681 gtgatggagc gcgggtccac ccgggaactc ttcaccaacc ccctgcatcc ctatacccag
     1741 gcgctctggc gttccatccc caccatcgac gggccgcgca cccggctcaa caccatcgcc
     1801 ggcatgatgc ccaagcccta cgaggtcccg agggggtgcg tgttcttcgg ccggtgcccc
     1861 gtgggcgtgg agggcgtgtg caacgccgct ccgccgcccg tggtggaagt cgcgccgggt
     1921 catgcggtgc actgcgtgaa ggcggcgggg aaatgaacgg agaaccggca gtccttctgg
     1981 atgtcaacgg cctgaagaag tacttccccg tggagggcgg cgcccttggc cgcggacgcg
     2041 gctgggtccg agccgtcgac gacgtgagcc
//
LOCUS       DASRZS010000349         3430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_191595, whole genome shotgun sequence.
ACCESSION   DASRZS010000349 DASRZS010000000
VERSION     DASRZS010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191595"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VFH83_08480"
     CDS             <1..1098
                     /locus_tag="VFH83_08480"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HET6486443.1"
                     /translation="REWVEYCNGTGDSTLTEERRRNGSPEPFGIRYWGIGNELWGCGG
                     HLQPTAYAAEVKRYLTFLNHFGVTRIACGPRGTNPFELRRDWTLDFFREYARVQHPSQ
                     RPVDGFALHFYTRNPAFGGDLAYSPEEYYASIQETLRMSDLIRETRAGMDAYDPGREI
                     GLVVDEWGAWHPQARGDTGLEQQNTMRDALLAALTLDIFVRNSDIVAMANIAQTVNVL
                     QAMVLTKGERMVVTPSYHVFEMYRRHQAAESVRVIIDAPTVRVGATAGPPAVAGSASL
                     KDGRLFLTLTNAHLEEAADVEVALLGAERPSARGVTGRQLAGGDIHEHNTFERPETVH
                     PVPIAPPWKGDHRATLRLPAHSVSALELQLS"
     gene            1204..2817
                     /locus_tag="VFH83_08485"
     CDS             1204..2817
                     /locus_tag="VFH83_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486444.1"
                     /translation="MKRLLVFGILLAIVSPAFAQLTTDEQKAGFHDGGYPIVDKTVTL
                     RIFAEKAPLHRKAFNDLPVPAKYERLTNVHIDWIEVPTTAMQEKKNLMLASNDLPDAF
                     SMPMPDYDLVTYGSAKVFIPLQDLIKRYMPNYQRVIAQKPDIPKFTTAPDGNIYSLAR
                     LNEGSWMRQLNIVSIYKPWVDKLGKAMPRTTEEFYDLMVAMKNTDFNGNGKAVIPIGM
                     AMGDLALGVPRSGPWYVYYAFGLPAQDTGTDNNVANLFVEDGKVKFAPADERFKQATI
                     FLNRLWKAGLIEPEAFTLTYAQMEAKLRAQPVTYGVVSVWNLQDEFTPGADPRADQYV
                     PLPFFTAPGVAKPAVYRQPYPGWNRGELVITRADKDPAVTARWVDTMYEPYASMEWIE
                     GMIGSRLQKDAKGVVTMKDPPAGMTTQEYRFSETQPGVPLAATMELYRSLFPAPIYEM
                     KGATIDKYYAPYWPKQFWSNPLMTPTDMKQLNAIDSDIRNLVTKTQARWITQGGVEAE
                     WDSYLKQMDALGLSAMLKIRQAGYDRLNQ"
     gene            2889..>3430
                     /locus_tag="VFH83_08490"
     CDS             2889..>3430
                     /locus_tag="VFH83_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6486445.1"
                     /translation="MAKSIPTSGPPRRARRGFLARAARSWELYLFLLPTLVYFAIFQY
                     GPLYGVQIAFRDYLGALGIWGSPWVGLQNFIRFFHSAFFVSLLANTVSLSLLILVFSF
                     PMPVIFALLLNQLGSQKYKRIVQTVSYAPHFISVVVFVGLIFLFSSPSTGIINILVSK
                     LGGPRIYYMGSLSWFRPVYLL"
BASE COUNT          656 a         1138 c         1061 g          575 t
ORIGIN      
        1 cgggagtggg tcgagtactg caacgggacc ggggactcca ccctgacaga agagcggcgg
       61 cgaaacggtt cccccgagcc cttcggcatc cgatactggg gcatcggcaa cgagctctgg
      121 ggatgcggtg ggcacctgca gcccacggcc tacgccgccg aagtgaagcg ctatctcacg
      181 ttcctgaatc acttcggggt caccaggatc gcctgcggtc cgcgaggcac caatccgttc
      241 gagctgcggc gcgattggac cctggacttc ttccgggagt atgcccgggt gcagcatccg
      301 tcccaacgcc cggtggacgg cttcgcgctg cacttctaca cgcgaaaccc cgcgttcgga
      361 ggggacctgg cgtactcgcc agaggagtac tacgcaagca tccaggaaac gctgcgcatg
      421 agcgacctca tccgcgagac acgagcgggg atggatgcgt atgaccccgg ccgcgagatc
      481 ggcctggtgg tcgacgaatg gggtgcctgg catccccagg cccggggcga cacggggctg
      541 gagcagcaga acaccatgcg ggatgcgctc ctcgccgcgc tcaccctcga catcttcgtc
      601 cgcaattcgg acatcgtggc gatggccaac atcgcccaga ccgtcaacgt cctgcaggcc
      661 atggtgctga ccaaggggga acggatggtc gtgacaccgt cctatcacgt cttcgagatg
      721 taccgacgcc accaggcggc cgaatccgtc cgggtcatca tagacgcgcc aacggtccgt
      781 gttggcgcca cggccggtcc cccggcggtc gccggctcgg catccctcaa ggatggacgc
      841 ctcttcctga cccttaccaa cgcccacctg gaggaggcgg ccgacgtgga agtcgcgctc
      901 ttgggggcgg agcggccctc ggcgcgaggc gtgacggggc gccagctcgc cggcggcgac
      961 atccacgagc acaacacctt cgagcgtccc gagaccgtcc accccgtccc catcgcgccc
     1021 ccgtggaagg gagaccatcg ggcgaccctt cggcttcccg cgcattcggt ctcggcgctg
     1081 gaactgcagc tatcctgacc ccggggatcc gcaagaagag gatcgcagaa aaaactggac
     1141 ttctgcaggc tgccgtggga gaataggggc ccgatgcgtc ccatcgatgg aaggaggagc
     1201 gcaatgaagc gactactggt gttcgggatt ctgctggcca tcgtctcgcc cgcgttcgcg
     1261 cagctcacga cggatgagca gaaggccggc ttccacgacg gcggctaccc gatcgtggac
     1321 aaaaccgtga cactgaggat cttcgcggaa aaagccccgc tgcatcgaaa ggcgttcaac
     1381 gacctgccgg tgccggccaa gtacgagagg cttaccaacg tccacatcga ttggatcgag
     1441 gtgcccacca cggccatgca ggagaagaag aacctcatgc tggccagcaa cgacctgccg
     1501 gacgcgttct cgatgcccat gccggactac gacctggtca cctacgggtc cgccaaggta
     1561 ttcatcccgt tgcaggacct gatcaagagg tacatgccca actaccagcg ggtcatcgcc
     1621 cagaaaccgg acattccgaa gttcaccacc gctccggacg gcaacatcta cagcctggcc
     1681 cgactcaacg agggaagctg gatgcggcag ctcaacatcg tcagcatcta caagccgtgg
     1741 gtggacaagc tcggcaaggc gatgcccagg accaccgagg agttctatga cctgatggtg
     1801 gcgatgaaga acacggactt caacggcaac ggaaaggccg tgatccccat cgggatggcc
     1861 atgggggacc tggcgctcgg ggtgccgcgc tccggcccgt ggtacgtgta ctacgccttc
     1921 ggtctgcccg cccaggacac cgggacggac aacaacgtgg cgaacctgtt cgtcgaggac
     1981 ggcaaggtga agttcgcgcc ggccgatgag cgcttcaagc aggccacgat cttcctgaac
     2041 cggctctgga aggccgggct gatcgagccg gaagccttca ccctgacgta cgcgcagatg
     2101 gaagccaagc tccgcgcgca gcccgttacc tatggcgtgg tctccgtctg gaacctccag
     2161 gacgagttca ctcctggggc ggatccccgc gcggaccagt acgtgccgct gccgttcttc
     2221 acggcgcccg gagtcgcgaa gccggcggta taccgccagc cgtacccggg gtggaaccgc
     2281 ggagagctgg tgatcacgag ggccgacaag gatccggctg tcaccgcccg atgggtcgac
     2341 acgatgtacg agccctatgc ttccatggag tggatcgagg gcatgatcgg cagccggctc
     2401 cagaaagacg ccaagggcgt ggtgaccatg aaggatcctc cggcgggaat gaccacccag
     2461 gagtaccggt tctccgagac gcagcccggc gtgccgcttg ccgcgacgat ggagctgtac
     2521 cggtccttgt tccccgcgcc catctacgag atgaagggcg cgacgatcga caagtactat
     2581 gcgccgtact ggccgaagca gttctggagc aacccgctga tgaccccgac cgacatgaag
     2641 cagctcaacg ccatcgactc ggacatccgc aacctggtga ccaagactca ggcccgatgg
     2701 atcacccagg gcggagtcga ggcggagtgg gattcctatc tcaagcagat ggacgccctc
     2761 ggcttgagcg ccatgctgaa gatccgccag gccggatacg accgcctcaa ccagtaaccg
     2821 gtaccagaga gctgccagca ccagggtccg acgccgccag gcgtcggacc ctctccggga
     2881 gtagaatcgt ggccaaatcc atcccgactt cgggcccgcc ccggcgggcc cgtcgcggct
     2941 tcctggcgcg cgccgcccga agctgggagc tgtatctctt cctcctcccg acgctcgtgt
     3001 atttcgccat cttccagtac ggacccctgt acggcgttca gatcgccttt cgggactacc
     3061 tgggagccct ggggatctgg ggtagcccct gggtaggact gcagaacttc atccggttct
     3121 tccactccgc ctttttcgtc agtctcctcg ccaacaccgt ctcgctgagc ctgctgatcc
     3181 tcgtgttcag cttccccatg cccgtgatct tcgcgcttct cctgaaccag cttgggagcc
     3241 agaagtacaa acgaatcgtc cagaccgtct cctatgcgcc gcatttcatc tcggtcgtcg
     3301 tgttcgtggg actgatcttc ctgttttcgt ctccatcgac cgggatcatc aatatcctgg
     3361 tctcgaagct ggggggcccc aggatctact acatgggcag cctgagctgg ttccgcccgg
     3421 tatacctcct
//
LOCUS       DASRZS010000350         3444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1350260, whole genome shotgun sequence.
ACCESSION   DASRZS010000350 DASRZS010000000
VERSION     DASRZS010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350260"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="VFH83_08495"
     CDS             <1..151
                     /locus_tag="VFH83_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6486446.1"
                     /translation="RSAHFSRSFVLPEDVEREKIAAEFKNGILTLTFPKVPAAQPKTI
                     EVKAT"
     gene            complement(209..1234)
                     /locus_tag="VFH83_08500"
     CDS             complement(209..1234)
                     /locus_tag="VFH83_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HET6486447.1"
                     /translation="MAINLGMWKRASTQLVRMDSKVEWDALDVVSKWLIATRSGVTTV
                     TLYSCVIAGLLAVHQGPFPWLTFIIVTLGLFLAHGANNLLNDYTDFRRGIDSDNYFRT
                     QYGVHPLVQGFWTRPVQIRWFVVSGVLALLAALYAIYITRFDPIIIGLVVYGSLMLLL
                     YTYPLKYFALGELTIFLIWGPIMIGGVYFVLTRQWSWEVALAGVPFGLSTASINIGKH
                     IDKSREDKVKRVGTLPVLIGETAARVLTFAAILLAYAVTLYLIVVPRFLTPVMLIVFF
                     AAPRGWKALVRLSKPRPLSAPPGYPVWPRWFSTVCFVHNRRFGNLFVLGIALDAVLRV
                     VLPSFWR"
     gene            complement(1327..2835)
                     /locus_tag="VFH83_08505"
     CDS             complement(1327..2835)
                     /locus_tag="VFH83_08505"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HET6486448.1"
                     /translation="MGKRAQVIAIDLGASGGKCFSGLFDDNGFSLREIHRFSHEPTTL
                     FVADRRGRVEERTCWNDLLIHANVVEGLRAYRKDVASSADGVGIDSWGTDGVFVTPDG
                     VVLGPMYSYRDHRLDGMIDQVKAKIDARRIYQVTGIHFQPFNMSNQLLWFVQNRGELL
                     KKGLRFLPAPTLFSFYLSGVTAVDSTWASVTQLMDCRKRKWSREVLRALGIPAWVMPE
                     IVAPATVVGQLRSELAQSVGLNAAPLIAVASHDTASAYTAAPVGNPATALIISSGTWS
                     LIGKILRKPNTGDEALDANLSNEGAIGGVRFLKNCMGTWLVQELRRAWRGQDGREIPW
                     DEMNRLTEAGQPFSAFLDPDDRGFYNPANMETAIQEFCKRTGQTAPGERGTLLRCVYE
                     SLALKYRMVAEQIATASGKPNKVVHIVGGGSRNELLNQMAANACRLPVVAGPEEATAA
                     GNAMAQGLALGAVGRLADIKELIRSAFPIREYKPKDAQTWEQAYQRYRQVVK"
     gene            complement(2929..>3444)
                     /locus_tag="VFH83_08510"
     CDS             complement(2929..>3444)
                     /locus_tag="VFH83_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HET6486449.1"
                     /translation="SRVERAEDYDRAVAEAFQYDTKVLVEENIEGRELECSVLGNDEP
                     IASVPGEIVTGGGHAFYDYQAKYIDENGALLLIPAPLDLGTAEAVRALAIRAFVSLSC
                     SGMARVDLFLRTTGELLVNEINTIPGFTSISMYPKLWEASGLPPARLVDRLIELALER
                     HRIETGLRTTP"
BASE COUNT          601 a         1135 c         1135 g          573 t
ORIGIN      
        1 gcgctcggcc cacttcagcc gcagcttcgt gctgcccgag gacgtggagc gggagaagat
       61 cgccgcggag ttcaagaacg gcatcctgac gctgaccttc ccgaaggtcc cggccgccca
      121 gcccaagacc atcgaagtca aagcaacgta aggctcgcag gcaaaaggtg aagacgggcc
      181 ccgaacctcg gggccccttt tttgcggctc agcgccagaa ggacggcagg acgactcgaa
      241 ggaccgcgtc cagcgcgatg ccgagcacga agagattccc gaaccgacgg ttatgcacga
      301 aacacacggt tgagaaccac cgcggccaca ccgggtagcc gggcggcgcg gaaagcggcc
      361 gaggcttgga gagccggacc agggccttcc acccgcgcgg cgcggcgaag aagacgatga
      421 gcatcacggg cgtcaggaac cgggggacca cgatcaggta cagggtcacg gcgtaggcca
      481 ggaggatggc cgcgaaggtg agcactcgcg ccgcggtctc cccgatgagc accggcaggg
      541 ttcccactcg cttcaccttg tcctcacggg acttgtcgat gtgctttccg atgttgatgc
      601 ttgccgtgct caatccgaag gggacgcccg ccagagccac ctcccagctc cactgccggg
      661 tgagcacgaa gtagaccccg ccgatcatga tcggtcccca gatcaggaag atggtgagct
      721 ctccgagggc gaagtacttc agcgggtagg tgtacaggag gagcatcagg gatccgtaga
      781 ccaccagtcc gatgatgatg gggtcgaacc gggtgatata gatggcatag agcgcggcca
      841 gcagggccag cactccgctc acaacgaacc atcggatctg gaccggccgg gtccagaacc
      901 cctggaccag ggggtgcacg ccgtactggg tgcggaagta gttgtcgctg tcgatgcccc
      961 ggcgaaaatc ggtgtagtcg ttcaggaggt tgttggcgcc gtgcgccagg aagaggccca
     1021 gcgtgacgat gataaaggtc agccagggaa agggaccctg gtggaccgcc aggagaccgg
     1081 cgatcacgca cgagtacagg gtcaccgtcg tgaccccgga ccgggtggcg atcagccact
     1141 tggagaccac gtccagggcg tcccactcca ccttgctgtc catgcggacc agctgggtgg
     1201 atgcccgttt ccacattccg aggtttatcg ccatttcatt ctccctttgc gagtcgctcg
     1261 ccagtccaat atacgaacgg caaagcgacc ggagaatctc aggcgccggg gctatttcgc
     1321 ctcggactac ttcaccacct ggcggtatct ctggtacgcc tgctcccagg tctgcgcgtc
     1381 cttgggcttg tactcgcgga tcgggaatgc ggatcggatg agctccttga tgtccgccag
     1441 ccgtcccacg gctcccagcg ccagaccttg agccatggcg ttgccggcgg cggtggcctc
     1501 ctccggtccc gccaccacgg gaagccggca ggcgttggcg gccatctggt tcaggagctc
     1561 gttgcgggag ccgccgccca cgatgtgcac caccttgttg ggcttgccgc tggccgtggc
     1621 gatctgctcc gccaccatcc ggtacttcaa cgccaggctt tcatagacgc aacgcaggag
     1681 cgtgccccgc tccccaggag ccgtctgacc ggttcgcttg cagaactcct ggatggccgt
     1741 ctccatgttg gccgggttgt agaagccgcg atcgtcggga tccaggaacg cggagaacgg
     1801 ctggcccgcc tcggtgagac ggttcatctc gtcccatggg atctcgcgcc cgtcctggcc
     1861 ccgccacgcc cgacgcagct cctgcaccag ccacgtgccc atgcagttct tgaggaagcg
     1921 cacgccgccg atggccccct cgttgctcag gttggcgtcc agcgcctcgt ctcccgtgtt
     1981 gggcttgcgc aggatcttgc cgatcaggga ccacgttccg gagctgatga tgagcgcggt
     2041 ggctgggttg ccgacggggg cggcggtata ggcggaggcg gtgtcgtggg atgccaccgc
     2101 gatcaacggc gcggcgttca gccccacgga ctgcgccagc tcggaccgga gctgacccac
     2161 cacggtggcg ggcgcaacga tctctggcat cacccaggca gggatgccca gggctcgcag
     2221 gacctcgcgg ctccacttgc gcttgcggca gtccatgagc tgagtgacgc tggcccacgt
     2281 ggagtccacg gccgttacgc cggacagata gaacgagaac agggtggggg ccggcaggaa
     2341 ccgcagcccc ttcttcaaca attccccgcg gttctggacg aaccagagga gctggttgga
     2401 catgttgaag ggctggaagt ggatccccgt gacctggtag atgcggcggg cgtcgatctt
     2461 cgccttcacc tggtcgatca tcccgtccag ccgatgatcg cggtagctgt acatgggccc
     2521 caggacgacc ccgtccgggg tcacgaagac cccgtcggtg ccccagctat cgatccccac
     2581 gccgtccgcg ctggatgcga cgtcctttcg ataggcccgc agtccttcca ccacgttggc
     2641 gtggatcagg agatcgttcc agcaggtccg ctcctccaca cgcccgcgcc gatcggccac
     2701 gaagagggtg gtcggctcgt ggctgaacct gtggatctcc cgcagcgaga agccgttgtc
     2761 gtcgaacagc cccgagaagc acttgcctcc cgaggcgccc aggtcgatgg cgatgacctg
     2821 cgcccgcttg cccatctcct cctcctgtgt ccgcaaacgg tagcaccgcg ggcccgtggc
     2881 aacaagcggg ggcgccaagc cgggggcgcc aagccggggg cgcccagcct acggcgttgt
     2941 ccgcagcccc gtctcgatcc ggtggcgctc cagggccagc tcgatcaggc gatccaccag
     3001 ccgggctggc ggaagcccgc tggcctccca gagcttggga tacatgctga tggacgtgaa
     3061 accggggatg gtgttgatct cgttgaccag cagctcgccg gtcgtcctca gaaagaggtc
     3121 cacccgggcc atccccgagc agctcaggct cacgaacgct cgaatggcaa gggcgcgcac
     3181 cgcctcggcg gttcccaggt ccagcggggc tgggatcagg agcagggccc cgttctcgtc
     3241 gatgtacttg gcctggtagt cgtagaaggc gtgccctccg ccggtgacga tttctccggg
     3301 gacggaggcg atgggctcgt cgtttccgag cacggagcac tccagctcgc gcccctcgat
     3361 gttctcctcc accagcacct tggtgtcgta ctggaacgcc tcggcgacgg cgcggtcgta
     3421 gtcctcggcg cgttccactc ggct
//
LOCUS       DASRZS010000351         4531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1351500, whole genome shotgun sequence.
ACCESSION   DASRZS010000351 DASRZS010000000
VERSION     DASRZS010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351500"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..928
                     /locus_tag="VFH83_08515"
     CDS             56..928
                     /locus_tag="VFH83_08515"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET6486450.1"
                     /translation="MSTKSREEQAGAEILGIVEEQVRMGPRVPGTPAHDRLTAILETR
                     LREYTDEVLLQEFSVSFHGRTIPCANVLGRFRSDPGSASTAVTLLGTHYDTRPRADRD
                     PDPELRERAIPGANDGGSGTAVLLHLLPWLRESHLARDVVVAFFDAEDLGNIDGKEFS
                     LGARYCAANPPWGRAPNEVVVLDMVGGADMVLDIDAHILRYPASRDLTGRIFQIGMMH
                     GWSAFARDKPDRFKYIISDHYPFVVEGIAACILIDIDYPQWHTQADQPQALSAASLGV
                     TEAVLSLFLSQPPA"
     gene            complement(882..2189)
                     /gene="hisS"
                     /locus_tag="VFH83_08520"
     CDS             complement(882..2189)
                     /gene="hisS"
                     /locus_tag="VFH83_08520"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET6486451.1"
                     /translation="MSELIEPRILKGFRDSMPQREAQRQQILSTLQEVFRLCGYAPID
                     TPVLEYAEVLLGKGGGETDKQVYRFTDHGGRDVALRYDLTVPFARFMAAHLGELPLPF
                     RRWHAAKAWRGENTQRGRYREFMQVDFDVVGADSASADLEILLLMRQSLAALGMQRSV
                     VHFSHRAVFNEFLSRLGLSARALDVLRIIDKERKIGAEKTRELLAEIAGAQTAPAILE
                     FTRIEPTPRETLSKIQKAVGAEHAAAVRLAEVLTSLDRLGATGSFILDPSITRGLDYY
                     TGMVFETFLMDLPDIGSICSGGRYNDLASLYCNQKLPGVGASIGVDRLMAAMEELGLL
                     KGDLSAASLLVLCLDETLLADYHRFAARFREAGMAAEVYPEAKKLAVQFGYAEKRGIP
                     LALVWGEEERKAGRVGLKDLRSRESFDGLTPEAAIGKAKELLR"
     gene            2366..3577
                     /gene="pcnB"
                     /locus_tag="VFH83_08525"
     CDS             2366..3577
                     /gene="pcnB"
                     /locus_tag="VFH83_08525"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HET6486452.1"
                     /translation="MLLRYGATANGEIEKKAHIYTQAEHGVEADRIDSDAVWVVRRLR
                     QAGFHAYLVGGAVRDLLLSRTPNDFDIATDAHPQQIRRCFRSARIIGRRFRLVHVYTR
                     GDRYMEVSTFRSRGALGTADHRDNPDQNNLFGTMEQDAERRDFTINALYYDPADQQII
                     DYVGGFTDIRQRRLRTLVPAETSFAEDPVRMIRAAKYSALVGFPIPLGMSLLIRRLRE
                     SLLGCSRERVTEEVYKILTSGGSVQVLDLLNRLRLFEVLFPSLSEQFRSDRLRFADSD
                     LAERLRRLDAGVPSGAVLDRSHLFGFLFRDLLVRRTDLLHGEDPEFLLQEYLRGASAP
                     LFPSKKDLAVGVELVLQEVLPSHRKREPAHPGRIGRPHHGEPPVRGPRPAGAKRRRRR
                     GGGRRPDAREA"
     gene            complement(3602..>4531)
                     /locus_tag="VFH83_08530"
     CDS             complement(3602..>4531)
                     /locus_tag="VFH83_08530"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6486453.1"
                     /translation="KILSQTRTDFVFVAWSDPSRSELLAFPKPTAAFNASLLHQGCIQ
                     FLKGFVDNPPVWLREGVATYLDAAVWDPKSSTFTPKANLAWLDTLRSMVRGETPNKLI
                     DFADLLQLSRERAQSQQQVFAPESWGLVQFLLNAPDRAYSRIFWDSLNAMDPKATLEA
                     NSLRVRKRAFSWVADQALQQDFSGYILALQTPNDLVRQGIDSYTNGDLASAEATFTKA
                     IDLEQGNITSWYYLGLIAYARKDYAKAEDDYLKAFQLGANAGVINYALGVNSFAAGKN
                     ADAQKYLTFAKQSDQAAYGDKVDVLLKRIQGTK"
BASE COUNT          755 a         1499 c         1502 g          775 t
ORIGIN      
        1 gccttctccg agcccgccgc ccgccgggtc gccctgaacc tgcgggaggg caggattgtc
       61 cacgaagtca cgtgaggagc aggccggcgc ggaaatcctt gggatcgtcg aagagcaggt
      121 aaggatgggg ccccgcgttc cgggaacccc ggcccacgac cgtctcaccg ccatcctgga
      181 gacccgactt cgagagtaca cggacgaggt cctgctccag gagttctccg tgtccttcca
      241 cggacgcacc attccgtgcg ccaacgttct cgggcggttc cgatcggatc ccggctcagc
      301 ctccaccgcg gtcacgctgc tgggcacgca ctacgatacg cgtcctcggg cggaccggga
      361 tccggatccg gagctgcggg aacgcgcgat cccgggcgct aacgacggcg gctcgggtac
      421 ggccgtgctc ctccatctgc tgccctggct ccgcgagtca cacctggcgc gggacgtggt
      481 cgtcgccttt ttcgatgcgg aggacctggg gaacatcgac ggcaaggagt tttccctcgg
      541 tgcgcggtac tgcgccgcca atcctccctg gggccgcgcg cccaacgagg tagtcgtcct
      601 ggacatggtg ggaggggcgg acatggtgct cgacatcgac gcccacatcc tgcggtatcc
      661 cgccagtcgg gacctgaccg gtcggatctt ccagatcggg atgatgcacg gctggtcggc
      721 ctttgcgcgg gacaagccgg accgattcaa gtacatcatc tcggaccact atccgttcgt
      781 ggtggaaggc atcgcggcct gcatcctgat cgatatcgac tacccgcagt ggcacacgca
      841 ggcggatcag ccgcaggcgc tgtcggctgc ctccctcggg gtcaccgaag cagttctttc
      901 gcttttccta tcgcagcctc cggcgtgagg ccatcgaagc tttcccgact ccgtaggtcc
      961 ttcagtccga cccggccggc cttgcgttcc tcttcgcccc acaccagggc cagcggaata
     1021 cctcgcttct ccgcgtagcc gaactgcacg gccagcttct tcgcctccgg atagacctcc
     1081 gccgccatgc ccgcctcgcg gaaccgggcc gcaaaccggt gatagtcggc gagcagggtc
     1141 tcatccaggc agagcaccag caggctcgcg gcggaaaggt cccccttcag gagccccagc
     1201 tcctccatgg ccgccatcag gcggtcgacc ccgatggagg cgccaacgcc gggaagcttc
     1261 tgattgcagt acagcgacgc caggtcgttg tagcgcccgc cggagcagat ggaaccgatg
     1321 tccggcaggt ccatcaggaa cgtctcgaac accatccccg tgtagtagtc cagacctctc
     1381 gtgatggaag gatcgaggat gaacgatccg gttgccccca gacggtcgag ggacgtcagg
     1441 acctcggcca gacgcactgc cgccgcatgc tccgcgccaa cggccttctg gatcttggac
     1501 agggtctctc gaggcgtcgg ctcgatgcgc gtgaactcga ggatggccgg ggccgtctgc
     1561 gctccggcga tctccgcgag cagctcgcgg gttttctccg cgccgatctt ccgctccttg
     1621 tcgatgatcc gaagcacgtc caaggcccgg gcggacagcc ccagccgcga gaggaactcg
     1681 ttgaacaccg cccggtgcga gaagtgaacc accgaccgct gcatgcccag cgccgccagg
     1741 ctttgccgca tgagcaggag gatctcgagg tccgccgagg cgctgtcggc gccgaccacg
     1801 tcgaaatcga cctgcatgaa ctcgcggtac cggccccgct gggtgttctc gccgcgccag
     1861 gccttggcgg cgtgccaccg gcggaagggg agggggagct cccccaggtg cgcggccatg
     1921 aaccgggcga aggggaccgt aaggtcgtag cgcagcgcaa cgtcccgacc cccgtggtcc
     1981 gtgaaccggt acacctgctt gtcggtttcc ccgccgccct tccccaggag cacctctgcg
     2041 tactccagca ccggagtgtc gatgggggcg tacccacaga ggcgaaacac ctcctggagc
     2101 gtggagagga tctgctggcg ttgcgcctct ctctggggca tggagtcgcg gaaccccttg
     2161 aggatccgtg gttcgatgag ctcgctcata tgaggccagt agtgcccagg ccgccggctc
     2221 tgtcaaggca cggcacccct ggcacccctg gcacgacgcc cctggcaccc ctggcacgac
     2281 gcccctggcg gacacccctt tcagcttgac aatcgccgag accgcggtac gataggagga
     2341 gaaacgcaac gatcgggaaa caccattgct tctgagatac ggcgctacag caaacggcga
     2401 gatcgagaag aaggcgcaca tatataccca ggcggagcac ggcgtggagg ccgaccggat
     2461 cgactccgac gccgtgtggg tggtccgtag gctgcgccag gcgggcttcc acgcatacct
     2521 ggtaggcgga gcggtgcggg accttctgct gtcacggacc cccaatgact tcgacattgc
     2581 caccgatgcg catccccagc agatccgccg atgcttccgc tcggcgcgga tcatcggccg
     2641 tcggttccgg ctggtccacg tctatacccg cggggaccgg tacatggagg tgagcacctt
     2701 ccgttcccgc ggcgcgctgg gcacggcgga ccatcgggac aaccccgacc agaacaatct
     2761 gttcgggacg atggagcagg acgcagaacg gcgggacttc acgatcaacg cgctgtacta
     2821 cgaccccgcg gatcagcaga tcatcgacta tgtgggcggg ttcacggaca tccgccagcg
     2881 gcgtctgcgg acgctcgtgc ccgcggagac ctcgttcgcg gaggaccccg tgaggatgat
     2941 ccgggccgcg aaatactcgg cactggtggg gtttccgatc cccctcggga tgtccctgtt
     3001 gatccggcgc ctccgggagt ccctcctagg ctgctcccgc gagcgcgtca cggaggaggt
     3061 ctacaagatc ctcaccagcg ggggctcggt gcaggtgctg gacctgctga accggctgcg
     3121 cctgttcgag gtcctcttcc cgtcgctgtc cgagcagttt cggtcggacc gtttgcgttt
     3181 cgccgattcg gaccttgcgg agcgccttcg gcggctggac gcgggggtac catccggagc
     3241 cgtcctggat cggagccacc tgttcgggtt cctctttcgg gacctcctgg tccgcaggac
     3301 ggacctcctg cacggtgagg acccggagtt ccttctccaa gagtacttgc ggggagcctc
     3361 cgccccgctc ttcccctcca agaaagatct ggccgtcggg gtggagctgg tgctgcagga
     3421 agtcctgcca tcccaccgca agcgcgagcc ggcgcacccg ggccgcatcg gccggcccca
     3481 tcacggtgag ccgcccgtca ggggacctcg acccgctggg gcgaagagga gacgcagacg
     3541 gggaggcgga cgccgccccg atgcaaggga agcctgaccg actgggaagg ctcgagcggt
     3601 actatttggt cccctggatt cgcttcagga gcacgtccac tttgtcgccg tacgcggcct
     3661 ggtccgattg cttggcgaac gtgaggtact tctgggcatc ggcgttcttc cccgcggcga
     3721 aggagttgac tccgagggcg taattgatca cgccggcgtt ggccccgagc tggaaggctt
     3781 tgaggtagtc gtcctccgct ttcgcgtagt ccttgcgggc gtaggcgatg aggcccaggt
     3841 aataccagga ggtgatgttg ccctgttcga ggtcgatggc cttggtgaag gtggcctcgg
     3901 ccgaggccag atcaccgttg gtataggagt cgatcccctg ccgcaccagg tcgttcgggg
     3961 tctggagcgc gaggatgtag ccggagaagt cctgctgcag cgcctgatcg gcaacccagg
     4021 agaacgcgcg ctttctgacc cgcagggagt tggcctccaa ggtggccttg gggtccatgg
     4081 cgttcaggga gtcccagaag atgcgggagt acgcccggtc cggcgcgttc aggaggaact
     4141 ggacgaggcc ccaactctcc ggcgcaaaga cctgctgctg gctctgggcc cgctcgcggc
     4201 tgagctgcag gaggtcggcg aagtcgatca gcttgttcgg ggtctctccc cggaccatgg
     4261 aacgcagcgt atcgagccac gcgaggttgg ccttgggagt gaaggtcgag ctcttggggt
     4321 cccacaccgc ggcgtccaga taggtcgcta ctccttcccg aagccagacc ggcggattgt
     4381 cgacaaaccc cttcaggaac tggatgcacc cctggtggag cagcgaggcg ttgaacgccg
     4441 ccgtgggctt ggggaacgcc aggagctcgg agcggctggg gtcggaccaa gcgacaaaga
     4501 cgaagtccgt tcgcgtctgc gataggatct t
//
LOCUS       DASRZS010000352         6280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_967543, whole genome shotgun sequence.
ACCESSION   DASRZS010000352 DASRZS010000000
VERSION     DASRZS010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967543"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFH83_08535"
     CDS             complement(<1..237)
                     /locus_tag="VFH83_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486454.1"
                     /translation="MRKPYLLALFFCVSMAAFAQETTQFTAETDHYRVISETSQSQAE
                     DVARHMEGALALYNSVFHFDLSLLTTKFQVRVLKD"
     gene            complement(305..943)
                     /locus_tag="VFH83_08540"
     CDS             complement(305..943)
                     /locus_tag="VFH83_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486455.1"
                     /translation="MNLVARLRTLFPWLTRKRRTFALVGRSGTGKSFKARQVARRFRI
                     DLIIDDGLLIRGQKIVAGKSAKREKAILGAIRTAVFANKDQIEDVKRGLDQQGYSRLL
                     IIGTSRKMIQRIASTLGVLPIHRVITIEEVSSPQEIAQALKIRAEQGKHIIPVPAVEV
                     KRNYPHIFFESVKILLKSKKGLRKSDDEVVEKTVVRPEYGKWRRDEQAPVKP"
     gene            complement(1102..2511)
                     /locus_tag="VFH83_08545"
     CDS             complement(1102..2511)
                     /locus_tag="VFH83_08545"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6486456.1"
                     /translation="MIRHVTLIEPKNNHLHIFSRFELPRLGGVLLATILRDQGYEADA
                     LFASPEDALRAPLSADLVGISTITATAPGAYRIADELRRRGTPVVLGGPHASFLPAEA
                     LEHADFCVTGEGETALPRLVEALNGKASLESVPGLAWKDHGEIRTNPRAAPVEDLDSL
                     PFADFSLLRVNSGKPFLRPGHGHPTIPIQTSRGCPFDCTFCSVTGMFGRRYRHRSAAN
                     VLSELSRYDPRRCLLFFYDDNFTANPRRSKELLREMIRLRLGFKWSTQVRTDVAKDPE
                     MLDLMAEAGCIYLYIGFESVDPVALREMHKSQTVEDMRSAIREIHRRRIGIHGMFVFG
                     FDSDTPQTARATVDFALQERVDTAQFLILTPLPGSDFYGKVLQEGRLIDRAWDTYDAH
                     HVKYLPRGFSPWELQLAQIRAHARFYAPLRVAGRLVRGNLTSVVIGLYARGLNRRWQI
                     QERDYLNWLRMLRPAPMLG"
     gene            complement(2673..3620)
                     /locus_tag="VFH83_08550"
     CDS             complement(2673..3620)
                     /locus_tag="VFH83_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET6486457.1"
                     /translation="MASRASRILRVIVIVLLALGTALFLYLLINNFQFTRGRSIRVHF
                     SSVGDLNAGAWVRKSGLKVGSVTRIEPAEDEKTIIVTLTFRPGQIVRTTDKFALVSKG
                     ILGDVYVEQNPGPKDSPMATEGMLFEGTAAFNLGDLIGPDVLTMIGQLVDSVKGFVDL
                     LQQNSGTIASALKDLAKTSENLRVVSDRAVAATADLPTMANQIADSIAQLQKTVDELS
                     SAAHRVSVSLEGNITSSSDDLSASMKSIRQASDQINQTVSQLTAQQSVLSKLSAPSTS
                     QSLDVTLRNLQDISTNLLTVTKQAQTILQGVQDIFTSSH"
     gene            complement(3610..4359)
                     /locus_tag="VFH83_08555"
     CDS             complement(3610..4359)
                     /locus_tag="VFH83_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6486458.1"
                     /translation="MIQARGVTFAFPGHTVLSELSLDVEDSLATVVVGRSGIGKSVLL
                     KCITGLLKPASGTIRIDGDEVVGADRRKLLEIRGRVGMLFQEGALFDSMDVYDNVAFP
                     LVYHKSCSAAEVERRVTSYLELVGMLDSKRAMPRELSGGMKRKVAVARAMILEPRYLL
                     YDEPTAGLDPASAAIVETLIHQLQVARSITSLIVTHDIDLTRFLADRIALLEDGRIVS
                     CETREEAFREGSPVYEHFIERRERIRQGNGL"
     gene            complement(4356..5132)
                     /locus_tag="VFH83_08560"
     CDS             complement(4356..5132)
                     /locus_tag="VFH83_08560"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486459.1"
                     /translation="MAGSEAGQEWGHAGASALSMYGNFFFWLFREPFPVRQTGQQIVS
                     VMLEAFGVMMIAAFIEGGMFAWLAGWDGGKFSVINWSGAASVYLLLSESTTMVGAMIF
                     AARIGTAFTVEIGSMQMSGQLDALRLMAVEPTQYIVIPRVLASILSLVTMKALSDGVA
                     ILSCMSLCRWWFGISFSIYLQHVWEILRHPILTTSYIRAAFMGFFIAVNSCGLGFYFN
                     GGAVELGRTTTKSIVINLLVVIVIDTTYGVIDSIFGLSTV"
     gene            5243..5725
                     /locus_tag="VFH83_08565"
     CDS             5243..5725
                     /locus_tag="VFH83_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HET6486460.1"
                     /translation="MLPQRAVDLLSRILSNPVFLAALSSWFLAQILKSCIMIFRNRPR
                     TAKEILVNIFWATGGMPSSHSAVVASLATSAGFVEGPNSTLFFVTLFYAVLTFRDAMG
                     VRRAAGAQAKVINQIIRDLSTKRPLRARPVKEIHGHTMAEVFVGALLGFFIAVAFCTL
                     "
     gene            5947..>6280
                     /locus_tag="VFH83_08570"
     CDS             5947..>6280
                     /locus_tag="VFH83_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486461.1"
                     /translation="MAILLLPSLPGSSPRPSQPWRRYHTILVRSDSQGPDGMEGLVRR
                     LKAAAGPGVVTELTAPVDFWDFSGVATVAYARLDSRLDPLDPRRDPYLNGLRSYLRTA
                     GASAEWRIA"
BASE COUNT         1094 a         2024 c         2050 g         1112 t
ORIGIN      
        1 gtccttcagc acgcggacct ggaacttcgt ggtcaggagc gacaggtcga agtggaagac
       61 actgttgtag agggccagcg cgccctccat gtgacgagca acatcctccg cctgggactg
      121 gctggtctcg gaaatgaccc ggtagtggtc cgtctccgcc gtgaactgag tggtctcctg
      181 cgcgaaggca gccatcgaaa cgcagaagaa cagggccagc aggtacggtt tccgcatact
      241 gcctcctcct aggatcatag cccccgtgtt ttgggctgtc aaatcaattt tcccgatccg
      301 tcgatcaggg cttcaccggt gcctgctcgt cccgtcgcca cttcccgtac tcgggtctca
      361 ccaccgtctt ctcgacgacc tcatcgtcgc tcttgcggag tcctttcttg gacttcaaca
      421 ggatcttcac ggactcgaaa aagatgtggg ggtagttccg cttcacctcg acggccggca
      481 ccgggatgat gtgcttcccc tgttcggccc ggatcttcag cgcctgggcg atctcctgcg
      541 ggctggagac ttcctcgatc gtgatgacgc ggtggatggg cagaaccccc agcgtggagg
      601 cgatccgctg gatcatcttt cgagaggtcc cgatgatgag caggcgcgag tagccctgct
      661 ggtccaggcc ccgcttcacg tcctcgatct ggtccttgtt ggcgaagacc gcggtgcgga
      721 tggcgcccag gatcgccttc tcccgtttgg ccgacttgcc ggccacgatc ttctggccac
      781 ggatgagaag cccatcgtcg atgatgaggt cgatgcggaa tcgacgggcc acctgtctcg
      841 ccttgaagct cttgccggtt ccacttcggc ctaccagggc gaacgtgcgg cgctttcgcg
      901 tcagccaggg aaacagagtc ctgaggcgtg ccacaaggtt catggagcga ggttctgtcc
      961 ttgcctccca ccatacacca cggcgctcgg gttgggaacg gccgcggttg ggaggagacg
     1021 gccgccttcg gcaagagatg gccggggcgg gagcagacgg ccgcttcgga gaccgccgct
     1081 tcggagaccg ccgcccgttg ctcagcccag catcggggcg ggacgaagca tgcgcagcca
     1141 gttcaggtag tcgcgctcct ggatctgcca gcggcgattg agcccccgag catacagacc
     1201 gatcacgacg ctggtcagat tgccgcggac cagccgtccc gccacgcgaa gcggcgcgta
     1261 gaatcgggcg tgggcccgga tctgagccag ctggagctcc catgggctga agccgcgcgg
     1321 gagatacttc acgtgatggg cgtcatacgt gtcccaggcg cggtctatca gtctcccctc
     1381 ctggagcacc ttcccgtaga agtccgagcc aggaagggga gtgaggatga ggaactgggc
     1441 cgtgtccacg cgctcctgga gggcgaagtc cacggtcgct cgggcggtct gaggcgtatc
     1501 ggagtcgaag ccgaaaacga acatcccgtg gatgccgatc cgtcgtcggt ggatctcccg
     1561 gatcgcgctt cgcatgtctt ccacggtctg gctcttgtgc atctctcgaa gggcgaccgg
     1621 gtcgaccgat tcgaacccga tgtagaggta gatgcatccg gcctccgcca tgagatcgag
     1681 catctccgga tctttcgcca cgtcggtgcg gacctgggtg gaccatttga accccaggcg
     1741 cagccggatc atctcacgca gcagctcttt cgatcgtcgg gggttggcgg tgaagttgtc
     1801 gtcgtagaag aaaaggaggc aacggcgcgg atcgtaccga gagagctccg acagcacgtt
     1861 ggccgcggaa cggtggcggt accgccgtcc gaacatcccc gtgaccgagc agaacgtgca
     1921 gtcaaagggg cagccgcgcg aggtctggat ggggatggtt ggatggccgt gccccggccg
     1981 caggaacggc ttgccgctgt tcacccgcag cagggagaag tccgcgaagg gcaggctatc
     2041 caggtcctcc acgggcgcgg cgcgcggatt ggtgcggatt tctccgtggt ccttccacgc
     2101 cagacccggc acgctctcca ggctggcctt gccgttcaag gcttccacca accgggggag
     2161 cgcggtctct ccctcccccg tgacgcagaa gtcggcatgc tcgagcgcct ccgccggaag
     2221 gaagctggca tgcggtccgc ccagcacgac cggtgtgcct ctccgccgca gctcgtcagc
     2281 gatccggtag gcgccgggcg ccgtcgcggt gatggtggag atcccgacca ggtccgccga
     2341 gagcggagcg cgaagcgcat cctccggcga ggcgaacagg gcatccgcct cgtatccctg
     2401 atctcgcagg atggttgcca ggagcacccc accgagcctc ggcagctcga accgggagaa
     2461 gatgtgcagg tgattgttct tgggttcgat caaggttacg tggcggatca tacccccccc
     2521 tatgaagtga tcgttgaaag atgctttgta gcaacatata cgtatacata caggtttgtc
     2581 aagagggtca ccgcggcgga tgatggaacc cggcgggaac ggaggacgcg tggaggggaa
     2641 atggggcccg ggccgtcggc cgggccccgc gctcaatggg aggaggtgaa gatgtcctgg
     2701 accccctgaa ggatcgtctg cgcctgtttg gtgacggtca gaaggttggt ggatatgtcc
     2761 tgcaggttgc gcaaggtcac atccaggctc tggctggtgg agggcgcgct cagcttgctc
     2821 aggacggact gctgggcggt gagctggctt acggtctggt tgatctggtc actggcctgg
     2881 cggatggact tcatcgatgc ggaaagatcg tccgagctgg aagtgatgtt gccctcgaga
     2941 ctcacggaga cccggtgcgc cgcggaagac agctcgtcca cggtcttctg cagctgcgcg
     3001 atcgagtcgg cgatctggtt ggccatggtg ggcaggtccg cggtggccgc tacggcgcgg
     3061 tcgctcacca cgcgcaggtt ctccgatgtc ttggcgaggt ccttcagcgc ggatgcgatg
     3121 gtgcccgagt tctgctgcag caggtccacg aagcctttca cgctgtccac gagctggccg
     3181 atcatggtga gaacgtccgg cccgatcagg tcccccaggt tgaacgccgc cgtcccttcg
     3241 aagagcatcc cctcggtggc catcggggaa tccttgggac cggggttctg ctcgacatag
     3301 acgtctccca ggatgccttt ggacacgagg gcgaacttgt ccgtcgttcg gacgatctgg
     3361 cccggtcgga acgtcagggt cacgatgatg gtcttctcgt cctcggcggg ctcgatgcgg
     3421 gtcacggacc ccactttgag gccggacttg cggacccatg cccccgcgtt gaggtccccc
     3481 acgctggaga agtgcacgcg gatgctgcgg cctcgcgtga actgaaagtt gttgatcaag
     3541 aggtagagaa agagcgccgt gcccaacgcc agaagcacga tgacgatcac tcggaggatg
     3601 cgggatgctc tagaggccat tgccctgcct gatcctttct ctgcgctcga tgaagtgctc
     3661 gtacaccggc gagccctctc ggaaggcctc ttcccgggtc tcacaggaaa cgatccgccc
     3721 gtcctcgaga agggcgatgc ggtccgccag gaaccgcgtg agatcgatat cgtgcgtcac
     3781 gatgagggac gtgatcgacc gagcgacctg gagctggtgg atcagggtct ccacgatcgc
     3841 ggcgctggcg gggtccagcc cggcggtcgg ttcgtcatac aggaggtatc ggggctccag
     3901 gatcatggcg cgcgccaccg ccaccttccg cttcatcccg ccggacagct cccggggcat
     3961 ggcgcgcttc gagtcgagca tccccaccag ctcgaggtac gacgtcactc ggcgctccac
     4021 ctcggccgcg gagcaggact tgtgatagac cagcgggaac gccacgttgt catacacgtc
     4081 catggaatcg aagagcgcgc cctcctggaa gagcatccct acgcgcccgc ggatctccag
     4141 gagcttgcga cggtccgcgc ccaccacctc gtctccgtcg atgcggatgg tgccggacgc
     4201 cggcttcagg aggcccgtga tgcacttgag gagcaccgac ttcccgatcc cgctcctgcc
     4261 gacgaccacc gtggccagcg agtcctccac gtccagcgag agctccgaca gcaccgtgtg
     4321 ccccggaaag gcgaaggtca ctccccgggc ctggatcata ccgtgctcag gccgaagatc
     4381 gagtcgatga cgccgtaggt cgtgtcgatg acgatcacca ccaggaggtt gatgacgatg
     4441 gacttcgtgg tggtccgtcc cagctccacc gcgccaccgt tgaaatagaa gcccagtccg
     4501 cagctgttca cggcgatgaa gaatcccatg aacgccgcgc ggatgtagga cgtggtcagg
     4561 ataggatgcc gcagaatctc ccacacgtgc tggagataga tggagaagga gatcccgaac
     4621 caccagcggc acagggacat gcaggagagg atggccactc cgtcggacag cgccttcatc
     4681 gtcaccaggc tcaggatgga ggcgagcacc cgcgggatca cgatgtattg ggtcggctcg
     4741 accgccataa ggcgaagggc gtcgagttgg ccgctcatct gcatggaccc gatctccacg
     4801 gtgaaggcgg tcccgatccg ggccgcgaaa atcatggcgc ccaccatggt ggtggactcg
     4861 gatagaagga ggtagacgct ggcggccccc gaccagttga tgacgctgaa cttccccccg
     4921 tcccatccgg caagccacgc gaacatcccg ccctcgatga acgcggcgat catcatgacg
     4981 ccgaaggcct cgagcatgac gctgacgatc tgttgacccg tctgacggac ggggaacggc
     5041 tcacggaaca gccagaagaa gaagttcccg tacatgctga gggcagaggc cccggcgtga
     5101 ccccactcct gtccggcttc gcttcccgcc attgcgtcga gatagtagag acggaggtcc
     5161 cggctgtcaa ggttttgctc gcccgaggcc gacgctggcg ccgacgcttg acactgcccg
     5221 ggccgccggc gaaactgagc caatgctccc gcagcgtgcc gtcgatctgc tgtcgcggat
     5281 cctgagcaat ccggtattcc ttgcagcgct gtcgagctgg tttctcgcgc agatcctcaa
     5341 gtcctgcatc atgatcttcc gcaatcgccc gcggaccgcg aaggagatcc tcgtgaacat
     5401 cttctgggcc acgggaggga tgcccagcag tcactcggcg gtcgtcgcct ccctcgccac
     5461 ctccgcggga ttcgtggaag ggccgaactc cacgttgttc ttcgttacct tgttctacgc
     5521 cgtcctgacc tttcgggacg ccatgggggt gcgccgcgcg gcgggcgccc aggcaaaggt
     5581 catcaaccag atcatccgcg acctctccac caaacggccg ctgcgcgcga ggcccgtcaa
     5641 ggagatccat gggcacacca tggcggaggt cttcgtcggc gccctgctcg ggttcttcat
     5701 cgccgtcgcc ttctgcaccc tgtgagcccg ggcaaaccgg cgccccggcg cgcggcgccc
     5761 aagaaggcga aagccgcgac cacggccgaa aggcgtggct caacgccgcc gcggcgtcca
     5821 cggggaaccc ctgccggcgg actcctctcc aaggccacgg cgagccgacg ccgctccgcc
     5881 cgtgaagccg ctccgcgggg acgtacgctc caccccccgg acctggcgtt tgtcgcggcc
     5941 gtcgccgtgg ccatcctgtt gctgccgtcc ctccccggct cgtccccaag accgtcgcag
     6001 ccctggagga ggtaccacac catcctggtc cgatccgact cccagggacc ggatggcatg
     6061 gaaggcctgg tccgccgcct gaaggccgca gcgggaccgg gcgtcgtcac cgagctcacc
     6121 gcgccggtgg acttctggga cttcagtggc gtcgctacgg tggcgtacgc ccggctggac
     6181 tccaggctgg atcccctgga cccgcggcga gacccgtatc tcaacggcct tcggagctat
     6241 ctccgtaccg ccggcgcgtc cgccgagtgg cggatagcct
//
LOCUS       DASRZS010000353         8795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4429193, whole genome shotgun sequence.
ACCESSION   DASRZS010000353 DASRZS010000000
VERSION     DASRZS010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8795
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4429193"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="VFH83_08575"
     CDS             complement(<1..1470)
                     /locus_tag="VFH83_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6486462.1"
                     /translation="MWFRSIFLKTLRESWVAILGWGAGMGLLMFELLATVNSLVSNAA
                     NASVLTSVTASFAWAGDPVALDTAGGYATWKTAVMLPLTAIWALIAGSRILRGAEERG
                     IMDALLAQPRSRLRVAVESLLAVWVALLGQGLLVALITFGGGASAHAGVAFGASLLFG
                     LNLALTCGVFASIALLIGQFTVERTAASGITGGLLFFFIMLDMLHRVVPGTDWLSHLS
                     PIYYFNLSKPIVPSFGTNIGAMAVLLGISVVLSAAGILIFLRRDIGRTAVLSDRAAAR
                     RAARRPSVDREPSLPARAWSLRSLYARGLASLVRPTIWWTIGVAGFAGFSVVVVKQIE
                     DKLTSMIAGSEFFSQLMNKVGGGGVGTNGVLLSTMFIFLPMLVMAFAVTQASRWASDE
                     EEGRQELVLATPHPRLRVILSRFAALSTAAVFISGVTLAATAVAASASGLALDAGNLA
                     AATLSIIPQCLLMAAVGYLGAGWLRTAMETGLLSFLLVIW"
     gene            complement(1457..2380)
                     /locus_tag="VFH83_08580"
     CDS             complement(1457..2380)
                     /locus_tag="VFH83_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486463.1"
                     /translation="MKAVVEVERLTKSYGSKRGITNVSFSVNEGEAFGFLGPNGAGKT
                     TTIRLLMALLRADSGSARIEGMDCWSQSVAIKRVVGYVPGEPSLDPGMTGGQILQYFA
                     HLRGGVDQAYLKRLVARLDLDPSRKFRQYSTGNKRKVVLIQAFMHRPRLLILDEPTSG
                     LDPINQQEFNAMVSEAREAGCTLFMSSHILSEVEHTCDRVAIIREGELVRVGGVSELK
                     DIQRHEVLITFAAAAPADAFKSLRGVESVEPSQDGRTLRVAVNGDLDAVVKAAARFPV
                     LNLASREPSLEEIFLRYYKSGSAAEASHVVS"
     gene            complement(2384..3133)
                     /locus_tag="VFH83_08585"
     CDS             complement(2384..3133)
                     /locus_tag="VFH83_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6486464.1"
                     /translation="MAAQRGTKAGRRVRGGLRRRSPAGGRELREDRILEAAVSVLARW
                     GFRKTTVDDVAREAGVGKGTIYLHWKDKVELFRAAIWYASQKTIEEMMKRVGADPDGG
                     QFHRLWAHGLVAIYDNPLLSGVMTGRIDMAGGAIEALGSSTVNRLIGTSEEQIAAMQR
                     ANLIRRDVPTRVVLLLVGALKLGIISSSQLLPAGQVPTAQELTDGLSDLMRRWLEPEG
                     GGGVSAEGKAIMKEWMKMVVSIGTRDRRTED"
     gene            complement(3501..6218)
                     /locus_tag="VFH83_08590"
     CDS             complement(3501..6218)
                     /locus_tag="VFH83_08590"
                     /inference="COORDINATES: protein motif:HMM:NF011596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease"
                     /protein_id="HET6486465.1"
                     /translation="MAVRLSPINRERWRRFKAHRRGFLSLWIFLVVFALSLGAELIAN
                     DVPLLVQWNGRLYFPVLKSFTEKDFGGEFETAADYRDPHVQKLITSRGWILWPLIPFS
                     FHTINYDLPSPAPSPPTHVNWLGTDENGRDVLANLIYGFRISVLFGLTLAIVCSIIGV
                     IAGAIQGYYGGWVDILGQRFIEVWSGLPQLFLIIILSSIVQPNFWWLLLIMVLFGWMG
                     LVGLVRAEFLRARNFEYVLAAKALGMKDRRVIFRHVLPNAMVATLTFMPFILGGSLGT
                     LTALDYLGFGLPVGTASLGDLLQQGQSNLQAPWLGISAFVVLGGALVLLFFVGEAVRD
                     AFDPRRAMPSGVSAGEEESAAVEMPAGLPEPVGSGRSSTDGALLSIQGLQVGFRRGAV
                     VHRVVHGASLQVRENETLGLVGESGSGKTVTAQAILRLIPETLIAYPGGEILFEGQRV
                     LDLADSELQRLRGSRIGMIFQEPMSSLNPLHTVERQLTETLGLHQGLRGPRASQVALT
                     WLERVGLRDAERRLKAFPHQLSGGERQRVMIAMALANEPRLLIADEPTTALDVTIQAQ
                     ILALIKQLQRELRMAVLFITHDLGIVRRFADRVAVMHDGLIVETGATEEIFQNPRDPY
                     TRRLLDTEAARESPRADPSRPELLRTQDLKVWFPIQRGFLRRTTGYVKAVDGVEIRVC
                     RGQTLGIAGESGSGKTTLGRAALRLLRSNGEILFEGRPVNGMRDDELRALRRRMQIIF
                     QDPFGSLSPRMSVEQIIGEGLVVHGIGQPEERGRMIAEAMTEVGLDPSQRHRFPHEFS
                     GGQRQRIALARALVLKPSLLVLDEPTSSLDRTIQFQIIELLRRLQERHELAYVFISHD
                     LKVLKSLCHHLAIMKDGKIVESGPAGEVFAHPRTPYTRELLATAFQASD"
     gene            complement(6220..7335)
                     /gene="yejB"
                     /locus_tag="VFH83_08595"
     CDS             complement(6220..7335)
                     /gene="yejB"
                     /locus_tag="VFH83_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019083498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HET6486466.1"
                     /translation="MGHYILRRLLFMIPTLWAIVTINFLVVQVAPGGPVDQALANLSG
                     RRNGGLLQRVTGSSAGSEVSAQTQGAQGGKEGSSAYRGARGLDPQVVAEITKRFGFDK
                     PLAARYWDMLKRYVVFNFGDSLFLGRSVVSLIIERIPVSISLGLWSTLIIYLVSIPLG
                     IRKAVRHGSPFDLWTTTTMLVANSIPSYLFAIILIVFLAGGSYLKIFPLRGLVSDAWR
                     SFGLVHKVLDYFWHITLPTLATVIGGGATLTMLTKNSFLDEIHKQYVMTARAKGLTEK
                     RILRRHVFRNAMLLVISGFPAAFIGIFFTGALLIEPIFSLQGLGLLGYSSTLQRDYPV
                     MFGTLYMFTLLALVLTLISDLTYTVVDPRIDFGTRQT"
     gene            complement(7340..>8795)
                     /locus_tag="VFH83_08600"
     CDS             complement(7340..>8795)
                     /locus_tag="VFH83_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6486467.1"
                     /translation="FHLNPKARAQDGSPITADDVVFSFNKFFNEGVPQFKQYYAGVDK
                     VEAPDPLSVKFTLKDGDRALAVSLGQLNIIPKKYWQNRNFGDPTTDVPLGTGAYKIKD
                     YKMGQYVIWQRMPDYWATGLPVNKGQLNFDNLRYDYYGNETVAFEAFKAGEFDVYQES
                     VAKNWATMYTGPNFDSGAIVKEEIPHQIPQGMQALVFNIQKPIFKDRRVRQALNYAMD
                     FEWMNRNLFYGQYTRARSYFTNTPYEAKGLPGPDELKYLNPLKGKIPDEVFTTEYQPP
                     VTDGTGEIRDQVRQALAILKDAGWEVRNQKLVNVNTGDPFTFEVLLDSPSMQRVAVPI
                     QQNLARMGITMTIRQVDESQFINRLRNRDYDMIAGGYDANYYPSTDMKIVWRSTYLDS
                     TYNMAGVQDPAVDSLIDQIDANQDNFDNLLALGKALDRVLTWNYYVIPEWYLSKFRVA
                     YWNKFSRPPVRPKYALGLGTWWVDPAKEAKLPKK"
BASE COUNT         1541 a         2927 c         2892 g         1435 t
ORIGIN      
        1 ccagatcacc aggaggaagc tcaggagccc cgtctccatc gcggtgcgaa gccagcccgc
       61 tcccaggtaa cccaccgccg ccatgaggag gcactggggg atgatggaaa gggtggcggc
      121 cgccaggttg ccggcgtcca acgccaggcc ggacgctgag gcggccacgg cggtcgccgc
      181 gagggtcacc ccgctgatga acacggccgc ggtcgagagc gcggcgaacc ggctgaggat
      241 cacgcgaagc cgggggtgcg gggtggccag caccagctcc tggcgaccct cttcctcgtc
      301 ggaggcccac cgcgaggcct gggtgacggc gaacgccatg accagcatgg gtaggaagat
      361 gaacatggtg ctcaggagca cgccgttcgt ccccacgccg cctccgccaa ccttgttcat
      421 gagctgcgag aagaactcgg agccggcgat catggaggtg agcttgtcct cgatctgctt
      481 gaccaccacc accgagaagc ccgcgaaacc cgccacgccg atggtccacc agatcgtggg
      541 tcggaccagg gatgccaggc cccgggcgta tagagagcga agggaccagg cgcgcgcggg
      601 gagcgagggc tcacgatcca ccgaggggcg ccgggcggct ctccgagcgg ccgcgcggtc
      661 ggaaagcacc gcggtccttc cgatgtcccg acgcaggaag atcaggattc cagccgcgct
      721 caggaccacc gagataccca ggagcacggc catcgccccg atgttcgtgc cgaaggacgg
      781 cacgatgggc ttgctcaggt tgaagtagta gatcggagac aggtggctca gccagtccgt
      841 ccccggcacc acgcggtgca gcatgtccag catgatgaag aagaagagga gtccgcccgt
      901 gatcccggag gccgccgtgc gctccacggt gaactggccg atcaggaggg cgatgctggc
      961 aaacacgccg caggtaaggg ccaggttcag cccgaagagc agcgaagcgc cgaaggccac
     1021 gccagcatgc gcggaagccc cgccgccaaa ggtgatgagc gccaccagga gcccctggcc
     1081 caggagcgcc acccataccg ccaggaggct ctctaccgcc acccgaagcc tgcttcgagg
     1141 ctgggccagc agcgcgtcca tgatgccccg ctcctccgcc ccgcgaagga tccggctccc
     1201 cgcgatgagc gcccagatgg cggtcagggg aagcatgacg gcggtcttcc aggtggcgta
     1261 cccgcccgcc gtgtccagcg ccacggggtc cccggcccac gcgaaggagg cggtgacgga
     1321 ggtgaggacg gaggcgttgg ccgcgttcga caccagggag ttgaccgtgg ccaggagctc
     1381 gaacatgaga agccccatgc ctgcgcccca gcccaggata gcgacccagc tctctctcag
     1441 cgtcttgagg aagatgctac gaaaccacat ggctggcctc cgcggcgctg ccgctcttgt
     1501 agtagcggag gaagatctcc tccaggctgg gctcgcggct ggccaggttc agcaccggga
     1561 accgggccgc cgccttgact accgcgtcca gatccccgtt cacggccacg cggagcgtcc
     1621 gcccatcctg ggacggctcg acgctttcca cgccgcgaag ggacttgaac gcgtccgcgg
     1681 gcgccgccgc ggcaaaggtg atgagcacct cgtggcgctg gatgtccttg agctcggata
     1741 ccccgcccac gcggaccagc tctccctccc ggatgatggc cacccggtca caggtgtgct
     1801 cgacctcact caggatgtgg gaggacatga agagcgtgca tcccgcctcc cgcgcctcgc
     1861 tcaccatggc gttgaactcc tgctggttga tggggtccag accgctggtc ggctcgtcca
     1921 ggatgaggag ccgggggcga tgcatgaacg cctggatcag caccaccttg cgcttgttcc
     1981 ccgtggagta ctggcggaac ttgcggctgg ggtccaggtc cagccgcgcg acaagccgct
     2041 tcagataggc ctggtccacg ccgccccgca ggtgcgcgaa gtactgcagg atctgcccgc
     2101 cggtcatacc gggatccagg gacggctcgc cgggcacgta gccgaccacg cgcttgatgg
     2161 cgacggactg gctccagcag tccatcccct cgatccgcgc gcttcccgag tccgcgcgca
     2221 ggagcgccat aagcaggcgg atggtcgtgg tcttccccgc cccgttggga ccgagaaagc
     2281 cgaacgcctc accctcgttc acgctgaacg agacgttggt gatcccgcgc ttgctgccgt
     2341 agctcttggt gagcctctcg acttccacta ctgccttcat acctcagtcc tccgttctcc
     2401 gatctcgagt accgatgctg acgaccatct tcatccactc tttcatgatg gctttgccct
     2461 ccgcgctcac cccgccgcct ccctcgggct ccagccagcg gcgcatgagg tcgctgagcc
     2521 cgtcggtaag ctcctgcgcg gtcggcacct ggccggccgg caagagctga ctggaggaga
     2581 tgatgcccag cttcagcgcg ccgaccagga ggagcaccac gcgggtgggg acgtcgcggc
     2641 ggatcaggtt ggcgcgctgc atggccgcga tctgctcctc cgaggtcccg atcaggcggt
     2701 tcaccgtgga gctgcccaac gcctcgatgg cgccgccggc catgtcgatc ctgcccgtca
     2761 tgacgccgga cagaagcggg ttgtcgtaga tggccaccag accgtgcgcc cagaggcgat
     2821 gaaactgccc gccgtccggg tccgcgccca cccgcttcat catctcttcg atggtcttct
     2881 ggcttgcata ccagatcgcg gcccgaaaga gctcgacctt gtccttccaa tggaggtaga
     2941 tcgtcccctt tcccacgccg gcctcccggg ccacgtcgtc cacggtggtc tttcgaaagc
     3001 cccagcgcgc cagcaccgaa accgccgcct ccaggatccg atcctcccga agctcgcgac
     3061 cgcccgcggg ggatctccgc ctcagcccgc ctcggacccg cctgcctgcc ttcgtcccgc
     3121 gctgcgccgc caccgcacct ctcctgtcga gcgccccgcc accggagcgg ccgaatgacc
     3181 agatgaccag atttgccaaa tccagtcata cagtaccgtg ggacggtcgc cgcgtcaacg
     3241 acccggagcc gcaaccgggc atcttttagc gatcttttaa cgcgccgatg gtacggcgcc
     3301 ggcctttggg aagcgcgacg cggcgggcac catggcacgc ttgttgcctg aagcggagca
     3361 ccgcaggcat tccaatgacg attcactcgg gaacgatcgg ccccagggat ccggatggga
     3421 catcggtgcg catgagttcg cggggaccgg ctccgcgccg cccaaggccc ccacgggacc
     3481 gcacgtggtc ccctgatccg tcagtccgac gcctggaacg cggtggccag gagctcgcgc
     3541 gtgtacggag tccgcgggtg ggcgaagacc tcgcccgcgg gccccgactc gacgatcttc
     3601 ccgtccttca tgatggccag gtggtgacag agcgacttga gcaccttcag gtcgtggctg
     3661 atgaagacgt aggccagctc gtgcctttcc tgaaggcggc gcaggagctc gatgatctgg
     3721 aactggatcg tgcggtccag cgaggaggtg ggctcgtcca gcaccaggag ggacggcttc
     3781 agcacgagcg ctcgggccag ggcgatcctc tggcgctggc ccccggagaa ctcgtggggg
     3841 aaccggtgcc gctggctcgg atccaggccc acctcggtca tggcctcggc gatcatccgc
     3901 ccccgttcct ccggctggcc gattccgtgg accaccaggc cctcgccaat gatctgctcc
     3961 acggacatgc gggggctcaa gcttccgaac ggatcctgga agatgatctg catgcgccgg
     4021 cgcagcgcgc gcagctcgtc gtcccgcatg ccgttcaccg gccggccctc gaagaggatc
     4081 tcgccgttgc tgcgcaggag gcggagcgcc gcgcggccca gggtggtctt gccggaaccg
     4141 ctttcccccg cgatccccag cgtctgcccc cggcataccc ggatctcgac gccatccacc
     4201 gctttcacgt acccggtggt ccggcgcagg aagccccgct ggatcgggaa ccataccttg
     4261 aggtcctgcg ttcgaagcag ctccgggcgg gaaggatccg cccgtggcga ctcccgcgcc
     4321 gcctccgtgt ccaggagcct ccgggtgtac ggatctcgcg gattttggaa gatctcctcg
     4381 gtcgcgcccg tctccacgat cagcccgtcg tgcatgaccg ccacacggtc ggcgaatcgc
     4441 cggacgatcc ccaggtcgtg ggtgatgaag agcaccgcca tgcgcagctc gcgctgaagc
     4501 tgcttgatga gggccaggat ctgggcctgg atggtgacgt ccaaggcggt ggtcggctca
     4561 tcggcgatca ggagacgcgg ctcgttggcg agggccatgg cgatcatcac gcgctgtcgc
     4621 tcgccgccgg agagctggtg cgggaaggct ttcagacgtc tctcggcgtc gcgaagcccc
     4681 acacgctcga gccaggtgag agccacctgg gaggcccgcg gaccgcgcag tccctggtgc
     4741 aggcccagcg tctcggtcag ttgtcgctcc acggtgtgca gcggattcag ggaggacatc
     4801 ggctcctgga agatcatccc gatccggctt ccgcgaagcc gctgcagctc ggagtcggcc
     4861 agatcgagga cccgctgacc ctcgaaaagg atctctccgc ccgggtaggc gatgagcgtc
     4921 tcggggatca gccgaaggat ggcctgcgcc gtgacggtct tgccggagcc gctctcgccg
     4981 accagaccga gcgtctcgtt ctctcggacc tggaggctgg cgccgtgcac cacccggtgg
     5041 accacggcgc cccgacggaa gccaacctgg agcccctgaa tcgagagaag ggctccatcc
     5101 gtggacgacc gtcccgatcc caccggctcc ggaagcccgg ccggcatttc cacggccgcg
     5161 ctttcttctt ccccggcgct gacgccggac ggcatggccc gccgcggatc gaacgcgtcc
     5221 cgcaccgcct ccccgacgaa gaacagcagc accagcgcgc cgcccagcac cacgaacgcg
     5281 gagatcccga gccacggcgc ctggaggttc gactgcccct gctgcaggag gtcccccagg
     5341 gacgcggtgc ccacgggaag cccgaagccc aggtagtcca gggccgtcaa ggttcccagg
     5401 gaaccgccaa ggatgaacgg catgaacgtg agcgtggcca ccatggcgtt gggaagcacg
     5461 tggcggaaga tgacccgacg atccttcatg ccgagcgcct tggccgccag cacgtactcg
     5521 aagttgcgcg ctcggaggaa ctcggcgcgc accaggccca ccagccccat ccagccgaac
     5581 aggaccatga tgaggagcag ccaccagaag ttgggctgga cgatactgga gaggatgatg
     5641 atgaggaaga gctgcggcag gccggaccag acctcgatga agcgctggcc caggatgtcg
     5701 acccagccgc catagtagcc ctggatggcc ccggcgatca cgccgatgat cgagcacacg
     5761 atggcaagcg tgagcccgaa gaggaccgaa atgcggaaac cgtagatgag gttggcaagg
     5821 acgtcccgac cgttctcatc ggtgcccaac cagttcacgt gcgtcggcgg cgagggcgcc
     5881 gggctgggca gatcgtagtt gatcgtatgg aagctgaagg gaatgagcgg ccagagtatc
     5941 cagcccctgc tcgtgatgag cttctggacg tgcggatccc ggtagtccgc cgccgtctcg
     6001 aactccccgc cgaagtcctt ctccgtgaag ctcttgagca cggggaagta caaccggccg
     6061 ttccactgca ccagcagagg aacgtcgttg gcgatgagct ccgcgccgag ggagagcgcg
     6121 aacaccacaa ggaagatcca gagggaaagg aatcctcgcc ggtgcgcctt gaacctccgc
     6181 cagcgctccc ggttgatggg gctcaaccgg acggccatgt caggtctgcc tcgtcccgaa
     6241 gtcgatgcgg ggatccacga cggtgtaggt caggtcgctg atcagggtga ggaccagggc
     6301 cagcagcgtg aacatgtaca gggtcccgaa catcaccgga tagtcccgct ggagcgtcga
     6361 ggagtagccc aggagcccga gcccctggag ggagaagatc ggctcgatca gcagggcgcc
     6421 ggtgaagaag atcccgatga acgcggccgg gaatcccgag atcaccagga gcatcgcgtt
     6481 tcgaaacaca tggcgtctca ggatgcgctt ctcggtgagc cccttggccc gcgccgtcat
     6541 gacatactgc ttgtggatct cgtcgagaaa cgagttcttc gtgagcatgg tgagggtcgc
     6601 cccacccccg atgaccgtgg cgagcgtggg aagcgtgatg tgccagaagt agtcgagcac
     6661 cttgtggacg aggccgaacg acctccaggc gtccgacacg agcccgcgga gtggaaagat
     6721 cttgaggtag gagccgccgg ccaggaaaac gatcaggatg atggcgaaga ggtagctggg
     6781 gatggagttg gccaccagca tggtcgtcgt ggtccacagg tcgaaagggc tcccgtgcct
     6841 cacggccttg cggatgccca gcgggatcga cacgaggtag atgatcagcg tgctccacag
     6901 ccccagggag atcgagaccg ggatgcgctc gatgatcagg gataccacgc tgcggcccag
     6961 gaagaggcta tcgccgaagt tgaagacgac gtagcgcttc agcatgtccc agtagcgcgc
     7021 cgccagcggc ttgtcgaacc cgaaccgctt cgtgatctcc gcgaccacct gcggatccag
     7081 cccgcgcgcg ccgcggtagg cggaggaccc ctccttgccg ccctgcgcgc cctgcgtctg
     7141 ggcgctcacc tcgctgccgg cgctggagcc ggtcacccgc tgcaagagcc cgccattgcg
     7201 ccggccggag agattggcca gcgcctggtc cacggggccg cccggggcga cctggacgac
     7261 caggaagttg atcgtcacga tcgcccacag cgtggggatc atgaagagca gccgccgcag
     7321 aatgtagtgt cccaaggcgc tacttcttgg gcagctttgc ctctttggcg gggtccaccc
     7381 accaggtccc cagacccagc gcgtacttgg gccggaccgg aggccgggag aacttgttcc
     7441 agtacgccac tcggaacttg ctgaggtacc actcggggat cacgtagtag ttccaggtga
     7501 gcacccggtc cagcgccttc ccgagcgcca gcaggttgtc gaagttgtcc tggttggcgt
     7561 cgatctggtc gatcagggag tccaccgccg gatcctgcac cccggccatg ttgtaggtgg
     7621 aatccaggta cgtggaccgc cagacgatct tcatgtccgt gctggggtag tagttcgcgt
     7681 cgtatccgcc ggcaatcatg tcgtagtccc ggttgcgcag ccgattgatg aactgactct
     7741 catccacttg ccggatggtc atggtgattc ccatccgggc caggttctgc tggatgggca
     7801 ccgcgacacg ctgcatcgag gggctgtcca gcaggacctc gaaggtgaac gggtcaccgg
     7861 tattcacgtt gaccagtttc tggttgcgga cctcccagcc ggcatccttc aggatggcca
     7921 gggcctggcg gacctggtcg cggatctctc cggtgccgtc cgtcaccggc ggctggtatt
     7981 ccgtcgtgaa gacctcatcc gggatctttc ccttcaacgg attcaggtac ttcagctcgt
     8041 cgggacccgg caggcctttc gcctcgtagg gagtgttcgt gaagtagctg cgggcgcggg
     8101 tgtactgacc atagaagagg ttgcggttca tccattcgaa gtccatcgcg tagttcaggg
     8161 cctgtcggac ccggcggtcc ttgaagatgg gcttctggat gttgaagacg agggcctgca
     8221 tgccctgcgg gatctggtgc gggatctcct ctttcacgat ggccccggag tcgaagttag
     8281 gccccgtgta catggtggcc cagttcttgg cgacgctctc ctggtagacg tcgaactccc
     8341 ccgccttgaa cgcttcgaac gccaccgttt cgtttccata gtagtcgtag cgaaggttgt
     8401 cgaagttcag ttgtcccttg ttgacgggga gacccgtggc ccaatagtcc ggcatccgct
     8461 gccagatcac gtactgcccc atcttgtagt ctttgatctt gtaggcgccg gtgcccagag
     8521 gcacgtccgt ggtgggatcc ccgaagttgc ggttctgcca gtacttcttg gggatgatgt
     8581 tgagctggcc cagggagacc gccagcgccc ggtcaccgtc cttcagcgtg aacttcacgc
     8641 tcagcgggtc cggcgcctcc accttgtcca cgccggcgta gtactgcttg aactggggca
     8701 ccccttcgtt gaagaacttg ttgaaggaga acaccacgtc atccgccgtg atcggtgagc
     8761 cgtcctgggc ccgggccttg gggttcaggt ggaag
//
LOCUS       DASRZS010000354         4999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_967661, whole genome shotgun sequence.
ACCESSION   DASRZS010000354 DASRZS010000000
VERSION     DASRZS010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967661"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VFH83_08605"
     CDS             <1..391
                     /locus_tag="VFH83_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486468.1"
                     /translation="SIVSLQSVLSLPISSSLSVGGALGLDLVLRFPFDFRTDIPDRTA
                     GEAPTLAYFFQDGRFFYPETRLFVRWQAGPRIGLYGNIRVLYPIFHLWDNEGLSFLDQ
                     TMASAGLGFSIRLGPTPAPAAAPTSVK"
     gene            complement(384..1604)
                     /locus_tag="VFH83_08610"
     CDS             complement(384..1604)
                     /locus_tag="VFH83_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HET6486469.1"
                     /translation="MKIWIKFLIGTLVGFVVGLFLPVSAQGFFDRLSDLLLGVGRYVI
                     FPLVFFSLGIGTSELRQEKRLLRVYASALKYLALSAAALVVIGTLSVLLFPPERIPIT
                     VESQKAFAPVSVMGGLASIFPPNLFSVISRPGDFLLPVMVLSFLLGLNFDFDRQLTRP
                     VVQLFDSLSRIFYHLNSLIVELFAIAVIAIFAARVSHLGHGDQGMYTQIVIILGVDVA
                     LVAFGVFPAALYLLGVKENPYRWLYASLGPAIAGLATGDQYVCLGVMSKHGRESLGVP
                     RMVGSAVYPLFATLGRAGTAMVSSVSFILILRSYSSLEITFLQTLWVMLFSFLVSFLL
                     GAVPGIGAYYSIFMMCNLYGRGLQDGYLILKNIAPLLVAFGVFIDVLSASFSSLLIAR
                     EENVWTEVETESFI"
     gene            1740..2582
                     /locus_tag="VFH83_08615"
     CDS             1740..2582
                     /locus_tag="VFH83_08615"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HET6486470.1"
                     /translation="MKGVIITAGYGTRFLPVTKTIPKEMLPLVTRPSIDFVVEELAAS
                     GITEILFVTSRRKKALEDYFDREMELEGVFRASGDQARLERIAPPKVKCYFVRQAEMR
                     GTGDALLYARAFVGDEPFVAAYPDDLHFGEKPLALQLIEEWEKTGCSVLATLHDPPEI
                     NRYGVVSIAEDGRHVTGIVEKPPKGQEPSREASIGRFLYAPDFLPQLAIERERHRQGE
                     FFHTPALLALARKGRVVYRRVEGERLDTGEPGGYLEAIIRYARRDPALSRELDRILAA
                     DRAR"
     gene            2592..3131
                     /locus_tag="VFH83_08620"
     CDS             2592..3131
                     /locus_tag="VFH83_08620"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET6486471.1"
                     /translation="MGPARVALIGFMGCGKSTVGPILAGLLGWSFVDTDDLVVQQAGT
                     PIAEIFRLRGEPAFRKMETQALAELTLRSDVVVATGGGAPLQPANREFFTLQARTFHL
                     RVSFAEVLARTAGDTGRPLLAQGEPALQRLFDARLPVYDSLGWAVETDGRSPREIAAE
                     IRATLVRPRRWVGPGEGGG"
     gene            complement(3079..3834)
                     /locus_tag="VFH83_08625"
     CDS             complement(3079..3834)
                     /locus_tag="VFH83_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HET6486472.1"
                     /translation="MLLIPAIDLRGGRCVRLREGSFGDATTYVVEPTEVALSYAGAGA
                     RWIHIVDLDAAEGRGEDNLRVIEKIRASVGCRLEVGGGVRSEEQAARLLRLGVDRIVL
                     GTILARTPLLVRDWVSRHGARFAGGVDARNRRVRISGWTEEGGISDSKLAAQLGSIGI
                     RWLIYTNIARDGTLAGPDIESTSSVARAACLPTVLSGGIGTEDDVLRVGESADSLVVG
                     TILGKALYEGRIDLASLIRRLPQDRPTAWDEQA"
     gene            complement(3839..4354)
                     /locus_tag="VFH83_08630"
     CDS             complement(3839..4354)
                     /locus_tag="VFH83_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET6486473.1"
                     /translation="MSLNHCFEPGTVVNPLVSTSKYDALRELIRRAPVFGELGDRGAF
                     EAAVIEREKSQSTGFGHGVAVAHGRVREARRVLVALGLSAAGIPFQSHDGEPVHLLFV
                     IASPPDLSLDYLQALSTLIRCVREPSVRQSLLGSQDEPSLHAMIRHVFATELGRCSIP
                     SSGPPALATTS"
     gene            4666..>4999
                     /locus_tag="VFH83_08635"
     CDS             4666..>4999
                     /locus_tag="VFH83_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET6486474.1"
                     /translation="MAKTPAKKASIKAPEKLTSASLVGYLAEKNGLVRKDVKQVLEDL
                     FELIEMGVMRGERVAIGKVGKMFIRVRPARAARQGRNPLTGETIMIAAKPATRVPRFT
                     FSKTFKEAA"
BASE COUNT          904 a         1663 c         1690 g          742 t
ORIGIN      
        1 atccatcgtg tcgctgcagt cggtcctgag cctgcccatc tcctccagcc tctccgtggg
       61 aggagccctg gggctcgacc tcgtcctgcg attccccttc gacttccgca ccgacatccc
      121 ggatcgtacc gcaggagaag cgccgacgct ggcgtacttc ttccaggacg gtcggttctt
      181 ctatccggag acgcggctct tcgttcgctg gcaggcggga cctcggatcg gcctgtacgg
      241 caacatccgc gtgctctacc ccatcttcca tctctgggac aacgaagggc tttcgttcct
      301 cgaccagacc atggcttccg cgggcctggg attctccatc cgcctgggcc cgacgcctgc
      361 gccggcggcg gccccgacgt cggtcaaata aagctctcgg tctccacctc ggtccagacg
      421 ttctcttctc gcgcgatcag gagagaggag aacgaagcgg acaacacgtc gatgaacacc
      481 ccgaaggcga ccaggagcgg cgcgatgttc ttgaggatca gatagccgtc ctggagcccc
      541 cgcccgtaga ggttgcacat catgaagatc gagtaatagg cgccgatccc gggcaccgct
      601 cccaggagga acgacacgag gaaggagaac agcatcaccc acagcgtctg gagaaaagtg
      661 atctccaggc tggagtagga gcgcaggatc aggatgaagc tgacgctgga taccatggcc
      721 gtccccgcgc ggcccaacgt ggcaaagagc gggtacaccg ccgagccgac catccgaggc
      781 acgcccaggc tctccctgcc gtgcttgctc atcacgccaa ggcacacgta ctgatctccc
      841 gtggccagtc ccgcgatggc cgggcccagg gaggcgtaca gccagcggta ggggttctcc
      901 ttcactccca ggaggtagag cgccgccggg aacacgccga aagccaccag cgccacgtcc
      961 acgcccagga tgatgacgat ctgcgtgtac atcccctggt ctccgtggcc caggtgactc
     1021 actcgggccg cgaagatcgc tatgaccgcg atggcgaaga gctccacgat gagggagttc
     1081 aggtggtaga agatccgcga gagagaatcg aagagctgca cgacgggacg ggtgagctgt
     1141 cgatcgaagt cgaagttgag gccaagcagg aaggaaagca ccatcaccgg aaggaggaag
     1201 tcccccggac gggagatcac cgagaagagg ttcggcggga agatggatgc cagcccaccc
     1261 atcacgctga cgggggcgaa cgccttctgg gactccaccg tgatggggat gcgctccggc
     1321 gggaagagca gcacggagag cgtgccgatg accaccaggg cggcggccga caaagccagg
     1381 tacttcaacg ccgaggcgta gacgcgcagg agccgcttct cctggcggag ctcggaggtg
     1441 ccgatgccca gggagaagaa gaccagggga aagatcacgt agcggccgac gccgagcagg
     1501 agatccgaga gccggtcaaa gaagccttgg gcgctcaccg gaaggaacag acccaccacg
     1561 aagccgacga gcgtaccgat gagaaacttg atccagatct tcatagacct cccccgtgga
     1621 gcacgcgcgg tcaggccccg cgggcgagca gggcggctgg cccagagtag cccgactgcg
     1681 gggcgggcgt caaggaaacc gctttgggtt ggcgacgctt tgggctatac tcggtcgcca
     1741 tgaagggcgt catcatcacg gcgggctacg gcacccgctt cctgccggtc accaagacca
     1801 tccccaagga gatgctcccg ctcgtcacgc ggccctccat cgacttcgtg gtggaggagc
     1861 tggcggcctc ggggatcacg gagatcctgt tcgtgacctc gcggcgcaag aaggcgctgg
     1921 aggactactt cgaccgggag atggagctgg agggcgtctt tcgggcgtcc ggcgatcagg
     1981 ccaggctgga gcggatcgcc ccgcccaagg tgaaatgcta cttcgtgcgg caggccgaga
     2041 tgcgcgggac gggggacgcc ctcctgtacg cgcgcgcgtt cgtcggggac gagccgttcg
     2101 tggccgcgta tccggacgat cttcacttcg gcgagaagcc gctggcgctc cagctcatcg
     2161 aagagtggga gaagacgggg tgttcggtcc tggcaaccct ccacgacccg ccggagatca
     2221 accgctacgg cgtcgtctcc atcgccgagg acggccgtca cgtcaccggg atcgtggaga
     2281 agcctcccaa gggccaggag cccagccggg aggcatccat cgggcgattt ctgtacgcgc
     2341 cggactttct cccgcagctc gcgatagagc gcgaacgcca ccgccagggt gagttcttcc
     2401 acacccccgc cctgctggcc ctggcgcgaa agggccgcgt cgtgtatcgg cgggtggagg
     2461 gcgaacggct ggacaccggc gagccgggcg gttacctgga agccatcatc cgatacgcca
     2521 ggcgcgaccc ggccttgagc cgcgagctgg accggatcct ggccgcggat cgagcgcgat
     2581 aacggcccgc cgtgggtcca gcccgggtgg cgctcatcgg gttcatgggg tgcgggaagt
     2641 ccacggtggg cccgatcctg gcgggactcc tcggctggtc tttcgtggac acggacgacc
     2701 tcgtggtcca gcaggccggt acccccatag cggagatctt tcgtctccgc ggcgagccgg
     2761 cgtttcgcaa gatggagacg caggcgctcg ccgagctgac cctgcggtct gacgtcgtcg
     2821 tcgccaccgg cggcggcgcg cccctccagc ccgccaaccg ggagttcttc acgctccagg
     2881 cgcgcacctt ccacctccgg gtttcctttg cggaggttct ggcccgcacc gcgggagata
     2941 ccggacgccc gctcctggct cagggggagc ccgccctcca gcgcctgttc gacgcccgac
     3001 ttccggtcta cgactcgctg ggatgggcag tcgagacgga cggccgatcc ccgagggaga
     3061 tcgccgcgga gatccgcgct acgcttgttc gtcccaggcg gtgggtcggt cctggggaag
     3121 gcggcggatg aggctcgcca ggtcgattct gccctcgtag agggccttgc ccaggatcgt
     3181 ccccaccacc agggagtccg cgctctcccc cacccgcagc acgtcgtcct ccgtaccgat
     3241 ccctccggag aggacggtgg ggagacaggc ggcgcgggcc acggagctgg tgctctcgat
     3301 gtcgggaccc gccagggtcc cgtcccgggc gatgttcgtg tagatgagcc agcgtatgcc
     3361 gatggaaccg agctgcgcgg cgagcttgct gtcgctgatg ccgccctcct cggtccagcc
     3421 ggagatccgc accctgcggt tgcgcgcgtc cactccgccg gcgaagcgcg ccccgtggcg
     3481 cgaaacccaa tcccgcacca ggagcggcgt cctggcgagg atcgtcccca ggacgatgcg
     3541 atccaccccg agccgcaaga gccgcgccgc ctgctcctcc gacctcaccc cgccgccgac
     3601 ttccagccgg caccccacgg aggcgcggat cttctcgatg acccgaaggt tgtcctcacc
     3661 gcggccttcc gcggcgtcca ggtccacgat gtggatccag cgcgcgccgg caccggcgta
     3721 cgagagcgcc acctcggtcg gctccacgac gtaggtagtg gcgtctccga agctgccctc
     3781 tcgaaggcgg acgcagcggc cgccgcgcag gtcgatggcg gggatcagca gcacaagatc
     3841 agcttgtcgt cgcgagggcc ggcgggccgg aagacgggat ggagcagcgg cccagctccg
     3901 tcgcgaacac atggcggatc atggcgtgga gggacggctc gtcctgggag cccaggaggg
     3961 actggcggac ggatggctct cggacgcagc ggatcagcgt cgagagcgcc tgaaggtagt
     4021 ccagcgacag gtcgggcggg gaggcgatga cgaacaggag atgcaccggc tcgccgtcgt
     4081 gagactggaa cgggataccg gccgcggaaa gccccagcgc cacgaggacc cggcgggctt
     4141 ccctcacgcg gccgtgcgcc acggccaccc cgtgaccgaa cccggtgctc tgggacttct
     4201 cccgctcgat gacggccgcc tcgaaggcgc cccggtcccc cagctcgccg aacaccggcg
     4261 cgcggcggat gagctcgcgg agggcgtcgt atttgctggt gctgacgaga ggattgacga
     4321 ccgttcccgg ttcaaaacag tggttcaaac tcatagtgct cgatcggacc tcattctacc
     4381 cgggcgccgc tccctgtcaa cccggcccag gttccgcgcc cgtcgctccg cacccgtacc
     4441 ggcgcgccag gccttcaccg cggcgcgcgc acggaaaatc ccgacagcga atgcacggag
     4501 ctcttgcgct aatatcgtca cggctctgga caatcggcca ttctcgttat atcctgcggg
     4561 caggaccgcg ccgaggcgcg gcagagccgt gcacggatac ggctttcgaa cgcgacggcg
     4621 accgtcggtc gcacatctgg actttcgcgg ggaggggaga agcaagtggc aaagacaccg
     4681 gcgaagaagg cgagcatcaa ggcgcctgag aagctcacca gcgcgtcact ggtgggatac
     4741 ctggcggaga agaacggcct ggttcgcaag gacgtgaagc aggtcctgga ggacctcttc
     4801 gagcttatcg agatgggcgt catgaggggt gagcgtgttg ccatcggcaa agtcggcaag
     4861 atgttcatcc gcgtccgacc agcccgcgcc gcgcggcagg ggagaaaccc cctgaccgga
     4921 gagacgatca tgatcgccgc gaagcctgcc acccgggttc cgagattcac gttcagcaag
     4981 accttcaagg aagcagccc
//
LOCUS       DASRZS010000355         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1352176, whole genome shotgun sequence.
ACCESSION   DASRZS010000355 DASRZS010000000
VERSION     DASRZS010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352176"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFH83_08640"
     CDS             complement(<1..361)
                     /locus_tag="VFH83_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486475.1"
                     /translation="MSNEIDLAQIKRAAHNAIPLTFKTYTLPHETEIYLDRVLESFLV
                     ELGQEKLKEPLSYCLRELAVNAKKANTKRVYFLEKGLDLNNDGDYEAGMKNFKQETFD
                     NIQYYLQKQKELGLYIKV"
     gene            complement(417..884)
                     /locus_tag="VFH83_08645"
     CDS             complement(417..884)
                     /locus_tag="VFH83_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET6486476.1"
                     /translation="MLASGLAFEREAIDAYRALREKLQKPGRCADPESLEGSLCHLLE
                     EEQMHEQMLADAAAGRLSMEELERTLKGHLYAGFDEIRPLQGEDAERWKEDLGAALAR
                     EEKTWIFYGNLGRMSKIPAAKRAFEVLASMEKEHVDILRRLLGAETASGTGKP"
     gene            complement(925..1209)
                     /locus_tag="VFH83_08650"
     CDS             complement(925..1209)
                     /locus_tag="VFH83_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HET6486477.1"
                     /translation="MAVNWEVLGSAFLMIFLAELGDKTQISTFAFASESKSPLSVFLG
                     AALALVCSSFIGVVLGGVVGRFVPGRIMKFVAAVVFLGFGAWTLIQAIRG"
     gene            1328..>2150
                     /locus_tag="VFH83_08655"
     CDS             1328..>2150
                     /locus_tag="VFH83_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding protein"
                     /protein_id="HET6486478.1"
                     /translation="MVSVQKVTNGVFWVEVPEADLRILCGCPADAVKHLIRRGLVGPM
                     KKRGVAFETGPNAILLSDTPIQKGAFANLSEFPLLQMFYRQGMLIPGHPNNTGRRPLL
                     AGLGDQVRSQAEYFMRGNYGLVSVEELEACGTPPEEARELFEIKKWFSFGSIRTVTDL
                     VDVRPVDAETVDLAPGCVLHRKGFNRYELLAGGHSVEVDLSLGPGEEFEPAYALAPAA
                     VRREAFSVIHVGEGDGWDPERPCMGSIVCGGGRLYLVDAGPHLTRSLDALGISLSE"
BASE COUNT          351 a          734 c          671 g          394 t
ORIGIN      
        1 tgaccttgat gtacagcccc agctctttct gcttctgcag gtagtactgg atgttgtcga
       61 aggtctcctg cttgaagttc ttcatcccgg cctcgtagtc cccgtcattg ttgaggtcga
      121 ggcccttctc caggaaatag acccgcttgg tgttggcctt cttggcgttg accgccagct
      181 cgcgcaggca gtaggacaac ggctccttca gcttctcctg ccccagctcg accaggaagc
      241 tctccagcac gcggtcgaga tagatttcag tctcgtgggg cagggtgtac gtcttgaacg
      301 tgagcgggat cgcgttgtgg gctgcacgct tgatctgcgc gagatcgatc tcgtttgaca
      361 ttgacccccc gtgtccgtct tcctgcgcca aagggtagtt gaaggacagg ggggtgtcaa
      421 ggttttcccg ttccggaggc cgtttccgcg cccaggagcc tcctcaagat gtccacatgc
      481 tccttttcca tggacgcgag gacctcgaat gccctcttcg ccgccgggat cttgctcatg
      541 cggccgaggt tgccgtagaa gatccaggtc ttttcctccc gcgcgagggc cgctccaagg
      601 tcttccttcc agcgctcggc atcctctccc tggagcggcc ggatctcgtc gaagcccgca
      661 tagaggtggc ccttcaatgt ccgctccagc tcctccatgg acaggcgccc cgccgcggcg
      721 tcggcgagca tctgttcgtg catctgctcc tcctccagga ggtggcagag gctgccctcc
      781 agggactcgg ggtccgcgca gcgtcccggc ttctgcagct tctccctcag ggcgcggtac
      841 gcgtcgatgg cttcccgctc gaatgccagg ccgcttgcca gcacctttct cagtatccag
      901 cgagagagca cgccggccat ccgatcagcc ccggatggcc tggatcagcg tccaggctcc
      961 gaacccgaga aagaccaccg ccgcgacgaa cttcatgatg cggccgggaa cgaacctgcc
     1021 aacgactccc ccgagcacca ccccgatgaa cgaggagcac acgagggcca gcgccgcgcc
     1081 gaggaagacc gacagcgggg acttggattc cgaggcaaat gcgaacgtgg agatctgcgt
     1141 cttgtccccg agctcggcga ggaagatcat caggaacgca gaccccagta cttcccagtt
     1201 caccgccacg ccggccctcc tgcggccgaa tcctaaccca gcgcccgctt cgatgccaac
     1261 gcccccgccg catcggcggc taccgcccgg ctccccccgg ggcgtatact cgccgggggg
     1321 ccaggacatg gtctcagtac agaaagtcac caacggcgtc ttctgggttg aggttcccga
     1381 ggccgacctg cggatcctct gcgggtgccc cgcggacgcg gtgaagcacc tgatccgccg
     1441 cggcctggtg ggaccaatga aaaagcgcgg agtggccttc gagacgggcc ccaacgccat
     1501 cctgctctca gacaccccca tccagaaagg cgccttcgcc aacctctcgg agttcccgct
     1561 gctgcagatg ttctaccggc agggcatgct gattcccggc cacccgaaca acaccgggcg
     1621 gcgcccgctc ctggcgggtc tcggggacca ggtgcgctcc caggcggagt acttcatgcg
     1681 cggcaactac ggcctggtat ccgtggagga gctggaggcg tgcggaacgc cgccggagga
     1741 agcgcgcgag ctcttcgaga tcaagaagtg gttcagcttc ggcagcatcc gcacggtcac
     1801 ggacttggta gacgtccgac cggtggacgc ggaaaccgtg gacctcgccc cgggctgcgt
     1861 tcttcaccgg aagggcttca accgctacga gctcctggcg ggcggtcatt ccgtggaggt
     1921 ggacctgtcc cttgggcccg gcgaggagtt cgagcccgcc tatgctctcg ctcccgccgc
     1981 cgtgcggcgg gaggcgttct cggtgatcca cgtcggagag ggcgacggct gggaccccga
     2041 gcggccctgc atgggcagca tcgtgtgcgg cggcgggcgc ctgtacctgg tggacgccgg
     2101 tccccatctc acgagatccc tggacgccct gggcatcagc ctctctgaga
//
LOCUS       DASRZS010000356         4797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1352806, whole genome shotgun sequence.
ACCESSION   DASRZS010000356 DASRZS010000000
VERSION     DASRZS010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352806"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VFH83_08660"
     CDS             complement(<1..1003)
                     /locus_tag="VFH83_08660"
                     /inference="COORDINATES: protein motif:HMM:NF014754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET6486479.1"
                     /translation="MDRLSKPRIHYLDGLRFRRAFIAGARQLIGNRDRLNAINVFPVA
                     DSDTGTNMAGTLGNVIEGIQPSRERSIGRMLGSIADLALSAARGCSGTILAQFFYGMA
                     QEFAGTSRLSTADFGYGVRKAVLYPWEAVSDPREGTILTVLRDWGDAIHRWSDRTQDY
                     VDLLFRARHAACRSLQATTAQLSPLQRAGVVDAGAQGLVHMLTGVTRFMANGTIREAD
                     RELMETPTEDVAAEVVEDPRFRYCTQFVIEGSDLDTRALRGELSGLGDSLIVAGSHTR
                     AKVHIHSNTPARAFHTVDQHGTIVVQRIEDMSAQFRAAHTTHKDIAIVVDSSCDMPQE
                     "
     gene            1132..2136
                     /locus_tag="VFH83_08665"
     CDS             1132..2136
                     /locus_tag="VFH83_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6486480.1"
                     /translation="MRRILLPIVFGVTAQLAVAQTPQLADGLYAEFKTERGTFICSLE
                     YQRAPMTVANFVGLAEGTIQANGVKGKKFYDGLTFHRVEPGFVIQGGDPNGDGSGGPG
                     YEFPNETSPLLKHDAAGVLSMANAGPDTNGSQFFVTLAPAPHLDGSYNVFGRVVQGMD
                     VVKAIQKGDHMVSVRILRIGGSAGGFVVTQKSFDDLVAKAKTASTQRHAKERADALSQ
                     IQRKWPKLITTRSGLMYEVLAKGSGGSPAATATVTVKYTGMLLDGTVFDSTDQHGGSA
                     SLQVNRVIPGWSEALQAMKRGEKRRLVIPPELAYGAQGYPGVIPGNAFLVFEVELVDF
                     "
     gene            2440..3816
                     /locus_tag="VFH83_08670"
     CDS             2440..3816
                     /locus_tag="VFH83_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HET6486481.1"
                     /translation="MTDASMTDDRIFQLRTRDIPKKWLNIQPSLPEPIPPMLNPADGK
                     PVPLEAFTPLFPMGLLEQEVSQAREIEIPDEVLRVYSLFRPTPLIRARFLEEALGTPA
                     VIFFKNESVTPTGSHKPNTAIPQAYYNKQEGVQKLVTETGAGQWGSALAFAGAHFGLP
                     INIFMVRASYEQKPYRKVMMQSYGATVHASPSKETQIGRKLSEDKKNYPGSLGIAISE
                     AVEMAASDAKAKYSLGSVLNHVLMHQTVIGLEARAVLEDLGLYPDIVIACHGGGSNFG
                     GIALPFMEDKISGKRKTEFIASEPLACPSLSKGIYAYDFGDAGKLIPLAKMYTLGHGF
                     IPPSVHAGGLRYHGAAPIVSLLVNKGLVRAEALRQRETFEAAVLFARTEGIIPAPESA
                     HAIASAIRAAKRCKETGERKVILFNLSGHGHFDMYAYEQYFAGKLEDYEYPEKEIRLA
                     EQDLPKVG"
     gene            complement(3849..4709)
                     /locus_tag="VFH83_08675"
     CDS             complement(3849..4709)
                     /locus_tag="VFH83_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486482.1"
                     /translation="METRLAQFTRGLYFDLQSIEGVQPLEIWNGVREQQDPFERAGVI
                     KNLYKIVVPLVNERCYGQPLSVHGEVASRTVSALLADYLDGSGLALFERFPYPPTGSE
                     DKKALYLQVKGRAAGVVAAADLPERPPQSRARVRRGGIEPRPRGGVEPRPGDAPQPVD
                     GIVPDSLFDFREMRLADNEFPYIIFTDKEFRVIHGLAVVYLWLIDLDSRFLQTLRNGP
                     GTEEIVEAYRKFAARVQRENPLAIHGKRMNREQLLQHVHEKMRYDDYLDVFEEVYRWL
                     EETQQKLQAG"
BASE COUNT          840 a         1563 c         1579 g          815 t
ORIGIN      
        1 cctcctgagg catgtcgcag gaggagtcca ccacgatggc gatgtccttg tgcgtggtgt
       61 gagcggcgcg gaactgggcg ctcatgtcct cgatgcgctg cacgacgatc gttccatgct
      121 ggtccacggt gtggaacgcg cgggccggcg tattggaatg gatgtggacc ttggcccgcg
      181 tgtgggaccc cgccacgatc agagagtccc cgagacccga aagctccccg cgcagggcac
      241 gggtgtccag gtcggagccc tcgatcacga actgggtgca gtagcggaac cgggggtcct
      301 cgacgacctc cgcggccacg tcttccgtgg gcgtctccat gagctcgcgg tccgcctcgc
      361 ggatggtccc gtttgccatg aaccgcgtca ctccggtcag catatgcacg aggccctggg
      421 cgccggcgtc caccacgccg gcccgttgaa gcggggagag ttgggccgtg gttgcctgca
      481 acgaccggca ggcggcgtgg cgcgcgcgga acagcaggtc cacgtaatcc tgggtccgat
      541 cgctccaccg atggatggca tccccccagt cccgcaggac cgtgaggatc gttccttccc
      601 gggggtcaga gaccgcttcc caggggtaca gcacggcctt tcgcaccccg tagccgaagt
      661 ccgccgttga caaccgggaa gtgccggcaa actcctgggc catgccgtag aagaactgcg
      721 ccaggatggt cccgctgcag ccccgtgccg ccgatagcgc aaggtccgca atagacccga
      781 gcatgcggcc gatggatcgc tcccggctgg gctggattcc ctcgatcacg ttgcccaggg
      841 taccggccat gttggtgccg gtgtcactgt ccgcgacggg gaatacgttg atggcgttga
      901 ggcgatcccg gttacctatg agctgcctgg caccggcgat aaaggcgcgt cggaatcgca
      961 gtccgtccag gtaatggatg cgcggctttg aaagcctatc cacgagctat cacggtagaa
     1021 gcgcgagggg ccaaagtcaa gcgaaatcgg gcacgggagc gccgcggcga gattgcatgt
     1081 ccttgactaa aaccgccttt catggcacgc cgattgggct tggaggaatc cgtgcggagg
     1141 atcttgttgc ccattgtctt tggagttacc gctcagcttg ccgttgccca gactccgcag
     1201 ctcgcggacg ggctgtacgc cgagttcaag acggagcggg gcacgttcat ctgctccctg
     1261 gagtaccaga gggcgcccat gaccgtggcc aacttcgtgg gcctggccga agggaccatc
     1321 caggccaacg gcgtcaaggg caagaagttc tacgacgggc tgaccttcca tcgcgtggag
     1381 cctggcttcg tcatccaggg cggggacccc aacggggacg gttcgggcgg accggggtac
     1441 gagttcccaa acgagaccag tcctctgctg aagcacgacg ctgcgggcgt cctctccatg
     1501 gcgaacgcgg gaccggacac caacggcagc cagttcttcg tcacgctggc gccggcccct
     1561 cacctggacg gaagctacaa cgtgttcggt cgggtggttc aggggatgga cgtggtgaag
     1621 gcgatccaga agggtgacca catggtctcg gtgcgcatcc tgcgcatcgg cggctcggcc
     1681 ggtggcttcg tggtcaccca gaagagcttc gatgatctgg tggcaaaggc caagaccgcc
     1741 tcaacccagc gccacgccaa ggagcgcgcg gacgcgctct cccagatcca gaggaagtgg
     1801 cccaagctga tcaccacccg ttcgggtctc atgtacgagg tgctggccaa gggatccggc
     1861 gggagcccgg ctgctaccgc aaccgtgacg gtgaagtaca cgggcatgct cctggacggg
     1921 accgtcttcg acagcaccga ccagcacggc ggatccgcct ccctgcaggt gaaccgggtc
     1981 atccccggct ggtccgaggc gctccaggcc atgaagcgcg gcgagaagcg gcggctggtc
     2041 atccctcccg agctcgccta cggcgcccag gggtacccgg gagtgattcc tgggaacgcc
     2101 ttcctggtgt tcgaggtgga gctggtcgat ttctagggcg accggctctg cggaaggcgg
     2161 gcttgacagg agccgggcgg tcatgctaga tagatccatg atgagctctg gtggttcgaa
     2221 gcgcttcgga ctcagctggt ggtggtggat cccggggagg aaaccgcggt ccgcctgacc
     2281 ttgtgaacga gctcaggaga ccgcaggcgg gttccctccc cgcgtgagag agagaccgcc
     2341 cgcggttttt tttacgcgga agacccgggc atggacccgg gtcttttgtt tttccggcac
     2401 ccgttgggcc gccgaaacag tctgggaaag gagcctttca tgaccgacgc ttcgatgacc
     2461 gatgatcgaa tcttccagct tcgcacccgg gatatcccca agaagtggct gaacatccaa
     2521 ccctccctgc ccgagcccat tcccccgatg ctcaacccgg cggacgggaa accggtgccg
     2581 ctggaagcgt tcacgccgct tttccccatg ggactcctgg agcaggaggt gtcccaggcg
     2641 cgagagatcg agatcccgga cgaggtcctc agggtctact ccctgtttcg tcccaccccg
     2701 ctcatccggg ccaggttcct ggaggaggcc cttggcactc cggccgtcat cttcttcaag
     2761 aacgagagcg tcacgccgac cggaagccac aagccaaaca ccgccatccc ccaggcctac
     2821 tacaacaagc aggagggggt ccagaagctg gtgacggaga cgggcgccgg tcagtggggc
     2881 agcgccctgg cctttgccgg agcacacttc ggccttccca tcaatatctt catggttcgc
     2941 gcttcctacg aacagaagcc ctaccgcaag gtcatgatgc agagctacgg cgccacggtc
     3001 cacgccagtc cgtccaagga gacccagatc ggccgcaagc tctcggagga caagaagaac
     3061 taccccggct cgctgggcat cgcgatcagc gaggcggtgg agatggcggc ctccgacgcc
     3121 aaggcgaagt actctctggg ctccgtcctc aaccacgtgc tcatgcacca gaccgtgatc
     3181 ggtctggagg cccgggcggt gctggaggat cttgggctct accccgacat cgtgattgcc
     3241 tgccacggcg gcggcagcaa cttcggcggc atcgccctgc ccttcatgga ggacaagatc
     3301 tccggcaagc gcaagaccga gttcatcgcc tccgagccgc tcgcctgccc atcgctgagc
     3361 aagggaatct acgcgtacga cttcggggac gccggcaagc tgatccccct ggccaagatg
     3421 tacaccctgg gtcacggctt cattcccccg tccgtccacg cgggtgggct gcgctaccac
     3481 ggcgccgctc ccattgtgtc cctgctggtg aacaaggggc tcgtccgcgc cgaggcgctt
     3541 cgccagcggg agacgttcga ggcggccgtg ctcttcgccc gaacggaagg catcatcccc
     3601 gcgccggagt ccgcgcacgc cattgcttcc gccatccgcg cggcgaagag gtgcaaggag
     3661 accggtgagc ggaaagtgat ccttttcaac ctctcaggtc acgggcattt cgacatgtac
     3721 gcgtacgagc agtactttgc cgggaagctc gaggactacg agtatcccga gaaggagatc
     3781 cggctggccg agcaggacct gcccaaggta ggctgagccg ggcgctgagg gcgcggcgcg
     3841 ccgcgccctc aaccggcctg gagcttctgc tgggtctcct cgagccagcg gtagacctcc
     3901 tcgaacacgt ccaggtaatc gtcgtacctc atcttctcgt gcacgtgctg gaggagctgc
     3961 tcgcggttca tgcgcttgcc atggatggca agggggttct cccgctggac ccgcgccgcg
     4021 aacttgcggt aggcctccac gatctcttcc gtaccggggc cgttgcggag cgtctggagg
     4081 aaccgggagt ccaggtcgat gagccagagg tacaccaccg ccaggccgtg gatgacccga
     4141 aactccttgt cggtgaagat gatgtagggg aactcgttgt ccgccagcct catctcccgg
     4201 aaatcgaaga ggctgtccgg aacgatcccg tccaccggct gcggagcatc ccctgggcgg
     4261 ggctctacgc cgccccttgg gcggggctct atgccgcccc gtcggacgcg ggcccggctc
     4321 tgcggcggcc tctcggggag gtcggccgcg gccaccacac cggccgctcg gcccttgacc
     4381 tggaggtaga gagccttctt gtcctcgctg ccggtaggcg ggtaggggaa gcgctcgaac
     4441 agcgcgaggc cggacccgtc caggtagtcc gccagcaggg cggacaccgt ccgggacgcc
     4501 acctcgccgt gaacggacaa gggctggccg tagcagcgct cgttcacgag cgggaccacg
     4561 atcttgtaga ggttcttgat cacgccggcc ctctcgaaag gatcctgctg ctcgcggacc
     4621 ccgttccaga tctccagagg ctgcacgcct tcgatgctct ggaggtcgaa gtacaggccg
     4681 cgcgtgaact gcgcaagccg ggtttccatc tctctgcctc cgcgacggcg tcgcccctca
     4741 ggactggccg gggacggcat gggccaacac ctcatggaag ctggagtccc ggaccag
//
LOCUS       DASRZS010000357         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2889910, whole genome shotgun sequence.
ACCESSION   DASRZS010000357 DASRZS010000000
VERSION     DASRZS010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2889910"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2020
                     /locus_tag="VFH83_08680"
     CDS             <1..2020
                     /locus_tag="VFH83_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6486483.1"
                     /translation="ANVFESASGSSDLVLTRPMANRMMKYFIEKNRQFPDVWSVFAPN
                     AFDGQDARFVNGAGTDQTGRFIPTWSRDTSGHGVLEPNVDYEKQGPGDYYLVPKARKK
                     ETVIDPYPYTLDGRSVMLASFAVPVLDPTGSFRGVVGVDLDLSDIQAQVKAARIGGYQ
                     GSYMHLISENGTIAASFVDSYVGKLASQAFADGGLVAAIKKGEAFTMELASADGNGKQ
                     MLTVGCPITVGSTGQNWLVVVNVPVDELMAASRQLTFLILLIAGVAILLLVFAIFGIS
                     RSITGPLTKGVTFAQVISTGDLSRTLDIHQKDEVGILADALNGMCVKLREMVSSVQAN
                     AEQIASSSEEISASTQKLSEDAQSEASTLEQTSASVEELTASVDQVAGHAQTQASAVE
                     QGTASMAQVQRSMEQVSSNFTEISGLAARSVENAVQGASAVREVVEGINLIATSSEKI
                     GGIISVISDIADQTNLLALNASIEAARAGEHGRGFAVVADEVSKLADRSAASTKEIDS
                     LIKESVRNVTLGVETAKGSQRAMEQIRDASKSVQDTIGVLSSSMTQQVESIQELAKAL
                     ASVNEMSLSISAATEEQTTNAKQVSRAVENVNEITQNAASAAEEMSSATTQLSGMAQE
                     LQRLMGQFRIGDGVDRIDLARAAAGNGNGRGNGNGNGNPVRAVEAAAS"
     gene            2095..>2604
                     /locus_tag="VFH83_08685"
     CDS             2095..>2604
                     /locus_tag="VFH83_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6486484.1"
                     /translation="MADQDPGPLALFHPIIAQWFLRTFGQPTDVQARAWPEIADGSHV
                     LVAAPTGTGKTLTAFLWGINQLVRGVWRPDAVRILYVSPLKALNNDIQRNLLVPLEQI
                     TRAFEQAGERLPTIRVQTRSGDTLPADRRRMLRKPPEILITTPESLNLLLSTPTGRTM
                     LSGLDTVILD"
BASE COUNT          495 a          855 c          849 g          405 t
ORIGIN      
        1 cgcgaatgtc ttcgaatcgg cctcgggctc atcggatctt gtccttacgc gacccatggc
       61 caataggatg atgaagtact tcatcgagaa gaatcgccag ttccccgatg tctggtccgt
      121 gttcgccccc aatgccttcg atggtcagga cgcccggttc gtcaacggcg ccggaacgga
      181 ccagacggga aggttcattc ccacctggtc ccgggataca tcgggacacg gagtcctgga
      241 gcccaacgtg gactatgaga agcagggtcc gggggactac tacctggtcc ccaaggcccg
      301 aaagaaggag acggtaatcg atccttaccc gtacacgctc gacgggcggt ccgtaatgct
      361 cgcttccttt gcggtgccgg tcctggatcc cacggggagc ttccgagggg tcgtgggtgt
      421 ggacctggat ctgagcgata tccaggcgca ggtaaaggcg gcgcggatag gcggttacca
      481 gggcagctac atgcacctga tctcggagaa cggcacgatc gccgccagct tcgtggattc
      541 ctacgtcggc aagctggcaa gtcaggcttt cgcggatggc gggctcgtgg ccgccatcaa
      601 gaagggagag gccttcacga tggagctggc ctccgccgac gggaacggca aacagatgct
      661 cacggtcggc tgtcccatca ccgtcggctc caccggacag aactggctgg tggtggtcaa
      721 cgtgcccgtg gatgagctca tggcggccag tcggcaactg acgttcctca tcctcctgat
      781 cgcgggggtg gccatcctgc ttctggtctt cgcgatcttc ggcatctccc gttccatcac
      841 cggcccgctc accaagggcg tgacctttgc ccaggtcata tcgaccggtg acctcagccg
      901 gaccctggac atccatcaga aggacgaggt ggggatcctg gcggacgcgc tcaacgggat
      961 gtgcgtcaag ctccgtgaaa tggtgtcttc cgtgcaggcc aacgcggaac agatcgcctc
     1021 ttccagcgag gaaatctccg ccagtaccca gaagctctcc gaggacgccc agagcgaggc
     1081 atccaccctc gagcagacca gcgcttccgt ggaggagctt accgcctcgg tagaccaggt
     1141 tgccggacac gcgcagaccc aggcctccgc ggtggagcag gggacggcat ccatggccca
     1201 ggtgcagcgg tccatggagc aggtctcgtc caacttcacg gaaatatcgg ggctggccgc
     1261 gcggtccgtg gagaacgcgg tgcagggcgc atccgccgtg cgcgaagtgg tggagggaat
     1321 caacctgatc gcgaccagct ccgagaagat cggcggcatc atcagcgtga tctcggacat
     1381 cgcggaccag accaacctcc tggcgctgaa cgcctccatc gaggccgcgc gagccggcga
     1441 gcatggccgt ggattcgccg tcgtggcgga cgaggtgagc aagctggcgg accgcagcgc
     1501 ggcctccacc aaagagatcg actccctgat caaggagagc gtccgcaacg tcacgctggg
     1561 ggtcgagacg gccaaaggat cgcaacgcgc gatggaacag atccgcgacg cctcgaagag
     1621 cgtgcaggat accatcggcg tgctctcctc ctccatgacc cagcaggtcg aatcgatcca
     1681 ggagctggca aaggctctcg caagcgtgaa cgagatgagc ctcagcatct ccgcggcgac
     1741 cgaggagcag accaccaacg cgaagcaggt ttcccgcgcg gtggagaacg tcaacgagat
     1801 cacgcagaac gccgcctccg ccgcggagga gatgtcctcc gccacgacgc agctctccgg
     1861 catggctcag gagctccagc gtctcatggg gcagttcagg atcggcgacg gcgtggaccg
     1921 gatcgacctg gcgcgggcgg ccgccggcaa cggcaacggc aggggcaatg gcaacggcaa
     1981 cggcaacccc gtgcgggccg tggaggcggc cgccagttga tctgacggga ccagggcgcg
     2041 agcggccgca cagttgacac ggccccggga cgtgctccta cactggagcc tcggatggct
     2101 gaccaggacc cgggaccgct cgctctcttc caccccatca tcgcgcagtg gttcctgcgc
     2161 accttcggac agccgaccga cgtccaggct cgagcctggc ctgagatcgc cgacggctcc
     2221 cacgtgctgg tggccgctcc cacgggcacg ggcaagaccc tcaccgcgtt cctgtggggc
     2281 atcaaccagc tcgtccgcgg cgtctggcgg ccggacgcgg ttcgcatcct gtacgtctct
     2341 ccgctgaagg cgctcaacaa cgacatccag cggaacctgc tcgtgcccct ggagcagatc
     2401 acccgggcgt tcgagcaggc gggagagagg cttccgacca tccgcgtgca gacccgaagc
     2461 ggggacacgc tcccggcgga ccgccggcgc atgctgcgaa agccgccgga gatcctgatc
     2521 accacgccgg agagcctcaa cctcctgctg tccacgccta ccggccggac gatgctgagc
     2581 ggcctggaca ccgtgatcct ggat
//
LOCUS       DASRZS010000358         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_195500, whole genome shotgun sequence.
ACCESSION   DASRZS010000358 DASRZS010000000
VERSION     DASRZS010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195500"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VFH83_08690"
     CDS             <1..281
                     /locus_tag="VFH83_08690"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6486485.1"
                     /translation="GVPSNPLPPYVLDLGGGGASLQNPRGLMTEGDLIEVSFSGEIGA
                     FTIVARVLRVSRRRGVMSVEFEHRSPEERNRIMRFLSAQAERRRPHAS"
     gene            670..>2210
                     /locus_tag="VFH83_08695"
     CDS             670..>2210
                     /locus_tag="VFH83_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET6486486.1"
                     /translation="MDRTGKTSDRLDAQVRELRCLLNVSSILADRETTIDQLLPRVVR
                     LIPPAYQYPGKVCVRIQCGDSIATSGGFAETPWEQSAIIAAEGKVAGSVQVCYLAETP
                     SEAEGPFLEEERELIDAVGEMIGDRLEREGIAQRLHLQEERLQAVLDSAPVILWATDR
                     EGNLTSVSGRMLASLGMEPGRFVGRPVESFLEGSALIRDDARRALRGEHVLSDLAARG
                     LTFESRVGPILDSTGKIDGVIGLLTDVTDRVQAESALKQTNRILEKVVSSTSFYMAFL
                     DGALKVVRVNPAFAAALGASPASCAGISFLDLLAEADGQDLFHEVLRSGEPRTAQEKP
                     FRLPVGQGAQTAYLDVDILPLDPGGQTRESLVVILVDRTLRRRATLELEKSRRELETL
                     AFHLEDLREEERRSIAREIHDELGGLLTALNMDLSLIDTAPGSDPSAGGAALERAKSL
                     VDKAVGMVRNIASSLRPQALDDFGLVVALEDLVEQFRRRAKIRCDLAISAEDRDVGGQ
                     TATAL"
BASE COUNT          392 a          670 c          785 g          363 t
ORIGIN      
        1 tgggagtgcc atccaacccg ctgccaccct acgtgctgga tctgggagga ggtggggcga
       61 gcctgcaaaa cccgcgaggg ctgatgacgg agggggatct gatcgaggtg tctttctcgg
      121 gagagattgg tgcgtttacc atcgtagcgc gcgtcctgcg ggtctccagg cgccgtggcg
      181 tcatgagcgt ggagttcgag caccgatcgc ccgaggagcg caatcgaatc atgcggttcc
      241 tgtccgccca ggccgaacgg cgccggccgc atgcctcctg aaccgccttg ggatgccgcc
      301 tcccggtctg gagtggctgc cgtgcatggt gacgaagtcc aacgggatgg acgatccgga
      361 ggggattcgt ccggcacgcc gccgccggaa aaagccggca cgttgaggac gatggcctct
      421 cggatgccat caacgaaatc caccgcggcg ggcgaaacca gcacgctcct cttcggttga
      481 tccgacgcgt tgtccagatc cacaacgaga tgacgaacga gccagatcct gtcgtcaaag
      541 aacacctctc ggagcaggcc gacgcggccg gtcatccgga ttcgaccatg cggtcacaat
      601 tccttggata gcgccttgcg cccgccccgc cattgcgcag tcgcgcgcat tggtactacg
      661 ctgggcgtga tggaccggac gggaaagaca tcagaccgtc tcgacgcgca agtccgcgag
      721 ctgcgatgtc tgctcaatgt ctcgtcgatc ctggcggacc gcgaaaccac gatcgaccag
      781 ctccttccgc gcgtcgtacg gctgatcccc ccagcgtatc agtacccggg taaagtctgc
      841 gttcgtatcc agtgcggaga cagcatcgcc acgagcggag gatttgcgga gacgccgtgg
      901 gagcaaagcg cgatcattgc ggcggagggg aaggtggcgg gcagtgtcca ggtctgctac
      961 ctggcggaga ctccatcgga agcggagggc ccgttcctgg aggaggagcg ggagctgatc
     1021 gatgcggtcg gggagatgat cggcgaccgc ctcgagagag aggggatcgc ccagaggctg
     1081 catctccagg aggaacgact ccaggcggtc ctggacagcg ctccggtcat cctatgggcc
     1141 accgaccggg aagggaatct cacctcggtg agcggccgca tgctggcgag cctcgggatg
     1201 gagccgggcc gattcgtcgg aaggccggtc gagtcctttc tcgaggggag tgcgctcatc
     1261 cgtgatgatg cccgtcgagc gctgcgggga gagcatgtgc tgtcggacct ggcggcgcgg
     1321 gggctgacgt tcgagtcgcg ggtagggccg attctcgact ccaccgggaa gatcgacggt
     1381 gtcatcgggc tcctgaccga cgtgacggac cgcgtgcaag ccgagtcggc gctcaagcag
     1441 accaaccgca tcctggagaa ggtggtctcc agcaccagct tctacatggc ctttctcgac
     1501 ggggcgctga aggtggtccg ggtgaatccg gcgttcgctg cggcgctggg agcaagcccc
     1561 gcgtcctgcg cgggcatcag ctttctggac cttctcgcgg aggcggacgg ccaggatctt
     1621 ttccacgagg tattgcggtc gggggagccc cgcaccgccc aggagaaacc gttccgactt
     1681 cccgtggggc agggcgcgca gaccgcctac ctggacgtgg acatccttcc gctggatccg
     1741 ggggggcaga cccgggaaag cctggtggtc atcctggtgg accgcacgct ccgaaggcgg
     1801 gcaaccctgg agctggagaa gtcccgacgg gagctggaga cgctggcatt ccacctggaa
     1861 gatctgcggg aggaggagcg gcgcagcata gcccgggaga tccacgacga gttgggggga
     1921 ctcttgaccg cgctcaacat ggatctatca ctgatcgaca cggcccccgg atccgatccg
     1981 tccgccggtg gcgcggccct ggagcgagcg aagtcgctgg tagacaaggc ggtcgggatg
     2041 gtccgcaaca ttgcatcctc tctgcgcccg caggcgttgg acgacttcgg cctggtggtt
     2101 gccctggagg acctggtgga gcagttccgc aggcgagcga agatccgatg tgacctggcg
     2161 atcagcgcgg aggaccggga cgtgggcggg caaaccgcca cggccctgtt
//
LOCUS       DASRZS010000359         4771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2890761, whole genome shotgun sequence.
ACCESSION   DASRZS010000359 DASRZS010000000
VERSION     DASRZS010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2890761"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VFH83_08700"
     CDS             complement(<1..736)
                     /locus_tag="VFH83_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486487.1"
                     /translation="MRSDSVRFGLKTKLIVFSVLLTVVMTLLVTLSIYQVVNRQLFNE
                     LRSKVLATVKIGADAIDRQRMGRLVALTRPDLPKEEVARLEDSADYRSLSDQLNRIRQ
                     TDPTVIRYVYTFIPTEDPNTTLFVVDADVLDQLASRAKGEKIDDSEISQIGTVFDVTD
                     YERARESIKGKTATLDDKYVWDDVYKVNSITAYAPILDGAGNLLAVMGIDMADVNART
                     ELADVTRLSLIIAVAAVILGLLASILL"
     gene            937..2097
                     /locus_tag="VFH83_08705"
     CDS             937..2097
                     /locus_tag="VFH83_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6486488.1"
                     /translation="MDELDSFLADLVSIDSVNPDLVPGASGEAKIAVFIAEWLRRKGL
                     EVHQEETAREGRWNVVAVARGRGGGRSLMLNAHMDTVGVAGMKEPFSAEIRGGRLYGR
                     GAVDTKGALAAYLCAMADVVGLGLPGDVVLTAVVDEEYASAGTEAVARKWKTDAAIVG
                     EPSGLEVVTAHKGFVWFDIETEGVAAHGSLPEVGVDAIARMGRVLTGLEAAQAALSAG
                     RKHPVLGPGSIHASLIQGGQELSSYPARCKLSLERRTIPGETPEAVEGSLREMLGHLE
                     RKDPTFKATLRRTFARDPMEARLDSPIVVSLARQVRRVTGKDAVFSGMSGWLDSALLS
                     AAGIETVVLGPSGDGLHGEVEWVDLESVHLCREIVRETAAEFCAVSAPGRGS"
     gene            complement(2072..2539)
                     /locus_tag="VFH83_08710"
     CDS             complement(2072..2539)
                     /locus_tag="VFH83_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HET6486489.1"
                     /translation="MTVAVITVSDRASAGQYEDRSGPAVEQEVLSRYPSAQVRRLVVP
                     DEPTRITAALEQSSDADFILTTGGTGVSPRDVTPETTRAFCDRELPGVAETLRAESLK
                     ETANAMLSRGYAGLRGTCIVVNFPGSVAAVRLCMKVIGPIMEHAVAMTRGQGH"
     gene            complement(2536..2997)
                     /gene="moaC"
                     /locus_tag="VFH83_08715"
     CDS             complement(2536..2997)
                     /gene="moaC"
                     /locus_tag="VFH83_08715"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET6486490.1"
                     /translation="MTMKLSHLDEQGKARMVDVSGKPRVLRTARATGTVRMAAETVRL
                     IRENQLQKGDALAVARIAGITGAKKTSELIPLCHGIRVDQVTVDLTLGEGRVEIESRV
                     ICTESTGVEMEALVAVSIAALTLYDMCKAVDKGMTIEAVRLLEKTKEEPSP"
     gene            complement(3007..4173)
                     /locus_tag="VFH83_08720"
     CDS             complement(3007..4173)
                     /locus_tag="VFH83_08720"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HET6486491.1"
                     /translation="MIGFEEALAMVRAVPGSGSVETVALARALGRVLALPVRSAIDSP
                     PFDKSAMDGFAVGAGADSGRYRILETVAAGGTARQTVRAGECVRIMTGAMIPTGAGRV
                     IRKEFVEEREGFIYPTAPEPGDNVIRRGSSVRAGEPVLLPKVIGPQDVGVLAASGAAS
                     VSVARPPSVGILSTGSEIVPPGGPLGVGQIYNSNGPQLVAHLEAVRCPATLLPTVVDS
                     PDLLTRALEAGLSSHEVVLLTGGVSEGDFDYVPRCLEDLGATIRFHRVSIKPGKPTLF
                     ASKGDQFLFGLPGNPVSTFVIFELLVKPFLYRRMGLDWEPPSWRGTLASAVRRKQAER
                     TEFLPVRLEAGLVHPVPYHGSSHLNALSLAQGLIRLDQGFTVLPEGTPVDVRPV"
     gene            complement(4194..>4771)
                     /locus_tag="VFH83_08725"
     CDS             complement(4194..>4771)
                     /locus_tag="VFH83_08725"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET6486492.1"
                     /translation="QRVALARALALAPSVLLLDEPTASVDPEGRPRIEELLRDAVRGG
                     TTVVMASHDPQTGYRLGDRLLRLEAGRVVPVEENVLRGVVESSDEQFTRFRTGSALIQ
                     APARTGRFQVAVLAMDEVVLSRERLHSSARNQLEGRVVRVEAWGGVLRVRVDCGAPID
                     ALITRASAAELGVVAGAPCVASFKASAVRLY"
BASE COUNT          806 a         1589 c         1618 g          758 t
ORIGIN      
        1 ccagcaggat cgaggcaagg aggcccagga tcaccgcggc gaccgcgatg atcagagaga
       61 ggcgggtgac gtccgccagc tcggtccgcg cgttcacgtc cgccatgtcg atgcccatca
      121 cggccagaag gttgccggcc ccgtccagga tcggggcata ggcagtgatg gagttgacct
      181 tgtagacgtc gtcccacacg tatttgtcgt ccagggtggc cgtcttgcct ttgatcgatt
      241 cccgggctct ctcgtagtcc gttacgtcga aaaccgtacc gatctgggag atctcgctgt
      301 catcgatctt ctctcccttg gcacggctgg caagctggtc caggacgtcg gcatccacga
      361 cgaagagggt cgtattggga tcctccgtcg gaatgaacgt gtagacgtag cggatgaccg
      421 ttgggtccgt ctggcggatc cggttgagct ggtcggagag ggaccggtaa tcggcggagt
      481 cctccaggcg cgccacctcc tccttgggca gatccggcct cgtcagagcc acgagacgac
      541 ccatccgctg gcggtcgatg gcgtccgcgc cgatctttac ggtcgcaaga accttggaac
      601 gaagctcgtt gaacaactgc cgattgacca cctggtagat ggacagggtg accagcaggg
      661 tcatgacgac ggtcagaaga accgagaaga cgatgagctt cgtcttcagg ccgaacctca
      721 ccgaatcgct gcgcatgtct cctccgcgta aagacttccg tcgagacaag gatagcccgc
      781 ggcgctggaa aacccgtggg aaaacccctg cttttcttgc cgaacggcga tggtatcctt
      841 tcggctccct gccgtcatcg cgaagcacga cgctcgaaga gcccggtata gagcgcggga
      901 ctcgcgcgca agcgaccgtc gtgctacaac gcctccatgg atgagctcga ttctttcctg
      961 gcggacctcg tctccatcga ttcggtgaat cccgatctcg tgccgggcgc aagcggggag
     1021 gcaaagatcg ccgtcttcat tgccgaatgg ctgcgccgca agggcctgga ggtgcaccag
     1081 gaggagaccg cgcgggaagg ccgctggaac gtggttgcgg tagcccgcgg ccgcggcggc
     1141 ggccgctccc tcatgctcaa cgcccacatg gacacggtgg gcgttgcggg gatgaaggag
     1201 ccgttctccg cggagattcg aggtgggcgg ctctacggca gaggcgcggt ggacaccaag
     1261 ggagccctcg ccgcgtacct ctgcgctatg gcggacgtcg tggggctcgg tctgccgggg
     1321 gacgtggtcc ttacggctgt ggtggatgag gagtatgcca gtgcgggtac ggaggcggtt
     1381 gcccggaaat ggaagacgga tgccgccatt gtcggagagc ccagcgggct ggaggtggtg
     1441 acggcacaca agggattcgt ctggtttgac atagagacgg aaggcgtcgc cgctcacggt
     1501 tccctgccgg aggtcggagt ggacgccatc gccaggatgg gacgggtgct gacgggactg
     1561 gaggcggctc aagcggccct gagcgccggc cgcaagcatc ccgttctggg gccgggatcc
     1621 atccatgctt ccctgatcca ggggggacag gagctatcca gctatccggc gcgatgcaag
     1681 ctcagcctcg agcggcggac catccctgga gaaactccgg aggcggtgga agggagcttg
     1741 cgggagatgc tcggccacct ggagcggaag gacccgacct tcaaggcaac actgcgccgg
     1801 acgttcgccc gagaccccat ggaagctcgg ttggactcgc ccatcgtcgt cagcctggct
     1861 cgccaggtgc gccgggtcac cgggaaggac gccgtgttct ccggcatgag cgggtggctg
     1921 gactccgcgc tgctttcggc cgccgggatc gagaccgtag tgctcggacc gtccggggac
     1981 gggcttcacg gggaggtcga gtgggtggac ctggagtcgg tgcacctctg tcgggagatc
     2041 gttcgggaga ccgcggcgga gttctgcgcc gtcagtgccc ctggccgcgg gtcatagcaa
     2101 cggcgtgctc catgatcggt ccgatcacct tcatgcagag ccgaaccgcg gcgacggacc
     2161 cagggaagtt caccacgatg caggttcccc tgagcccggc gtagcctcgg gagagcatgg
     2221 cgttggcagt ctccttcagg gactcggccc tcagggtttc ggccacgccc ggcagctcgc
     2281 ggtcgcagaa ggcgcgggtc gtctccggcg tcacgtccct cggagagacg cccgtgccac
     2341 cggtggtcag gatgaagtcg gcgtccgagc tctgctccag cgcggccgtg atccgcgtcg
     2401 gctcgtcggg gaccacgagg cgcctcacct gggcgctggg gtagcgagag agtacctcct
     2461 gctccaccgc gggaccggac cggtcctcgt attggccggc ggaggcccga tcggagaccg
     2521 tgatgacggc tacggtcatg gtgagggctc ctccttggtc ttctccagga gacggaccgc
     2581 ttcgatcgtc atgcccttgt ccacggcttt gcacatgtcg tacagcgtga gggcggcgat
     2641 ggaaaccgcc accagggctt ccatctcgac gccggtgctc tcggtgcaga tcacccggga
     2701 ctctatctcc acgcggccct cacccaacgt caggtccacg gtgacctgat ccacgcggat
     2761 cccgtggcag agggggatca gctccgaggt cttcttggcg cccgtgatcc cggcaatacg
     2821 ggcgacggcc aaagcatctc ccttctggag ctggttctcc cgaatcagcc ggaccgtctc
     2881 tgccgccatc cggaccgtgc ccgtggcccg ggctgttcgc agcacccggg gcttcccgga
     2941 cacgtccacc atgcgcgcct tgccctgttc atccaggtga ctcagcttca ttgtcagcct
     3001 ccgatgtcag accgggcgga cgtccaccgg cgtgccctcc ggcagcacgg tgaagccctg
     3061 gtccagccgg atcagcccct gcgccaggct cagggcgttg aggtgcgaag agccatggta
     3121 cgggacgggg tggaccaggc cggcctcgag gcgcacgggg aggaactcgg tcctctccgc
     3181 ctgcttcctt cgcacggcgg aggccagcgt cccccgccag gacggcggct cccagtccag
     3241 gcccatgcgt cggtacagga acggtttcac cagcagctcg aagatcacga acgtggacac
     3301 cggattgccc ggcaggccga acaggaactg gtctcccttg ctggcgaaga gcgtcggctt
     3361 gccgggcttg atcgagacgc gatggaaccg aatcgtggcc cccaggtcct ccagacatcg
     3421 ggggacgtag tcgaagtctc cttcggagac cccgccggtc agaaggacca cttcgtggga
     3481 cgagaggccc gcctccagcg cccgggtcag cagatcgggg gaatccacga cggtagggag
     3541 aagcgtggcg ggacagcgca cggcctccag gtgagccacg agctgaggcc cgttgctgtt
     3601 gtagatctgg ccgaccccga gcgggccgcc tggcgggacg atctccgatc ccgtggagag
     3661 gatccccacg ctgggcggcc gcgcaacgga gacggacgcc gccccggagg ctgccagtac
     3721 tcccacgtct tgagggccga tcaccttggg cagcagcacg ggctcgcccg cccggacgct
     3781 ggagccgcgg cggatcacgt tgtctcccgg ctcgggagcg gtcggataga tgaagccttc
     3841 ccgctcctcg acgaactcct tgcggatcac tcgcccggcc ccggtgggga tcatcgcacc
     3901 ggtcatgatc cggacgcact cgccggcccg caccgtctga cgggccgtcc ccccggccgc
     3961 cacggtctcc aggatgcggt agcgcccgga atcggcgccg gctcccaccg caaacccgtc
     4021 catcgcggac ttgtcgaacg gcggcgaatc gatggcggac cgcaccggca gcgccaggac
     4081 tcgtcccaac gcgcgggcca gggccacggt ttccaccgag ccggaccccg gaactgcgcg
     4141 aaccatcgcc aaggcctctt cgaaaccgat cacgggttca cctcaggctc acctcagtag
     4201 agacgaacgg cggaggcctt gaagctggcc acgcacgggg cacccgccac taccccgagc
     4261 tccgcggccg acgcccgagt gatgagcgcg tcaatgggag cgccgcagtc cactcggacc
     4321 cggagcacac cgccccaggc ctccacgcgg acgaccctgc cctccagctg gttacgcgcg
     4381 ctcgaatgca ggcgctcgcg ggagaggacc acctcatcca tcgcaaggac ggccacctgg
     4441 aaccgaccgg tccgagccgg agcctggatg agcgcggacc cggtccgaaa gcgcgtgaac
     4501 tgctcatcgc tcgattcgac cacgccgcgc agcacgttct cctcgacagg caccacccgc
     4561 cccgcctcca accgcaggag ccgatcgccc aaccggtagc cggtctgcgg atcgtgggag
     4621 gccatcacca cggtggttcc gccacggacg gcgtcccgca gaagctcttc gatccggggt
     4681 ctaccttccg ggtccacgct cgccgtcggc tcgtccagga ggaggaccga gggagccaac
     4741 gccagggcgc gggccagcgc gacgcgctgc t
//
LOCUS       DASRZS010000360         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2512153, whole genome shotgun sequence.
ACCESSION   DASRZS010000360 DASRZS010000000
VERSION     DASRZS010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2512153"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="VFH83_08730"
     CDS             complement(<1..1327)
                     /locus_tag="VFH83_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486493.1"
                     /translation="MSRRDLTRVCLGLQWDTATWNEAPSWWDFLLSRIQDTRVLWNLA
                     PLPPDASRRRPGVPRELAERVNQAGDAVSCRGFGGCYHPLLTLDELEKELAWGVKNPW
                     GTGITEVLGLQPTVLGPRLADLLREDALKAYTSHGFRWIAAPGPRAPARIGTLGLVSV
                     STVAVAPLRVGDPNLRKLRQVDGVDSVCMLDLSGLSSADALRLAFEELGGGVEPRPRG
                     GVEPRPAGGAALALVDRLKGTAEECPVLGSAPQPDWSALPAPTLRASLRACEVRHRKR
                     RNEDYREVLLLMGSTVDDALAASAAEPEPRRELRLVAHMLGEVVLAGRDFDVRLNGGR
                     FHGLVRKGSDLTPAVPAASYLRLRGRYRPFRGMSSFSFDSDEGTGLREELRLDGFADA
                     NLSIEYGFRGDSSFLTISVETRYPVLDPAGVVEEHAPLTLTLTEGREPAW"
     gene            1571..>2313
                     /gene="groEL"
                     /locus_tag="VFH83_08735"
     CDS             1571..>2313
                     /gene="groEL"
                     /locus_tag="VFH83_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET6486494.1"
                     /translation="MAKQLRFDEDARQKLLRGVEKMSTAVKVTLGPKGRNVVLDKKFG
                     APTVTNDGVTIAKEIELEDPFENMGAQLLKEVATKTNDVAGDGTTTATVLAYAMIKEG
                     LKAVAAGINPMDIKRGISKAVDIAVEDIKKSSKDIKEKEEIAQVASISANNDPEIGKL
                     IADAMEKVGKDGVITVEESKSLETNLEVVEGMQFDRGYSSAYFVTNRDTQTAVLENPY
                     ILIHDKKISGMKDLLPLLEKIAQGGKPLLL"
BASE COUNT          458 a          766 c          758 g          331 t
ORIGIN      
        1 cccacgccgg ctcgcggccc tccgtgagcg tcagggtcaa gggagcgtgc tcttccacga
       61 cccccgcggg atccaggacc ggataccggg tctcgacgct gatcgtgagg aaggaagagt
      121 ctccccgaaa gccgtattcg atgctgagat tggcgtcggc aaacccgtcc agccgcagct
      181 cctcccgaag ccccgtgccc tcgtccgaat cgaaggagaa cgagctcatt cctcggaacg
      241 gccggtagcg gccccgcagc cgaagatagg atgccgcggg cacggcgggt gtcaggtcgc
      301 tgcccttgcg caccaacccg tgaaaccgcc cgccgttgag gcgcacgtcg aagtcccgcc
      361 ccgccaggac cacctccccc agcatgtgag ccaccagccg cagctcgcgc ctgggctccg
      421 gctccgcggc ggacgctgcc aaagcgtcgt cgaccgtgga gcccatcaaa aggaggacct
      481 cgcggtagtc ttcgttccgc ctctttcggt gtcggacctc gcaggcgcgc aggctcgcgc
      541 gaagggtcgg ggcggggagg gcgctccagt cgggctgcgg ggccgagcca aggacggggc
      601 actcctccgc ggtccccttg agacggtcga cgagcgccaa ggccgcgcct ccggcagggc
      661 ggggctctac gccgcccctt gggcggggct ctacgccgcc ccccagctct tcgaaggcga
      721 gccgcagcgc atcagcggac gagagcccgc tcagatccag catgcacacg gagtccaccc
      781 catccacctg acgcagcttg cggaggttcg ggtcaccgac gcgcagcggc gcgacggcta
      841 ccgtggagac cgagacgagc cccagggtgc ctatccgcgc cggcgctcgc ggcccgggcg
      901 ccgcgatcca ccggaaaccg tgggaggtgt aggccttgag agcgtcctcc cgcagcaggt
      961 cggccagacg cggacccagg acggtcggct gcagccccag gacctccgtg atccccgttc
     1021 cccacgggtt cttgacgccc caggccagct ccttctccag ctcgtcgagg gtgagcaggg
     1081 ggtgatagca tccgccgaac ccacggcacg agacggcatc ccccgcctgg ttcacgcgct
     1141 ccgccagctc gcgggggacg ccgggacgcc ttcgagaggc atccggaggc agtggggcca
     1201 ggttccagag gacccgggtg tcctggatcc gagacaggag gaaatcccac catgacggag
     1261 cctcgttcca ggtggccgtg tcccactgaa ggcccaggca gaccctcgtg aggtcccgtc
     1321 ggctcattcc gtcctttatt accagaaggt ctctgaaagt caagccgacc gcggcccggc
     1381 cgagcgcggc ccggccgcta tcgggcctgt tgggggcgcg tggaatgtcg gcgaccgggc
     1441 gagaatctcc caaaagggct tgatgatccg cgcgtctttg tgtatcttag gcgcaaaagc
     1501 aacagacacg ctcaaggatc gaacgtgtgc gtgaactatt ttaacaccat gagttggaaa
     1561 ggggagacac atggctaagc agctccgttt cgacgaggat gcccgacaga agcttcttcg
     1621 gggcgtggag aagatgtcca ctgccgtaaa ggtgaccctc ggcccgaagg ggaggaacgt
     1681 cgtcctggac aagaagttcg gcgctccgac cgtcaccaat gacggcgtga ccatcgccaa
     1741 agagatcgag ctggaagacc cgttcgagaa catgggcgct cagcttctca aggaagtcgc
     1801 cacgaagacc aacgacgtgg ctggggatgg aaccaccacc gccaccgtcc tggcgtacgc
     1861 catgatcaag gagggcctga aagccgtggc cgcggggatc aaccccatgg acatcaagcg
     1921 cgggatctcc aaggccgtgg acatcgcggt ggaggacatc aagaagagct caaaagacat
     1981 caaggaaaag gaagagatcg cccaggtcgc ctccatctcc gccaacaacg atcccgagat
     2041 cggcaagctg atcgcggacg ccatggagaa ggtgggcaag gacggggtca tcacggtaga
     2101 ggagtccaag agcctggaga ccaacctcga agtggtggag ggcatgcagt tcgaccgcgg
     2161 gtattcctcg gcgtacttcg tgaccaaccg ggacacgcag accgcggtcc tggagaaccc
     2221 gtacatcctg atccacgaca agaagatctc cggcatgaag gacctccttc ccctgctgga
     2281 gaagatcgcc cagggaggaa agccgctgct cct
//
LOCUS       DASRZS010000361         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2893008, whole genome shotgun sequence.
ACCESSION   DASRZS010000361 DASRZS010000000
VERSION     DASRZS010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2893008"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFH83_08740"
     CDS             <1..561
                     /locus_tag="VFH83_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486495.1"
                     /translation="IQEEHQSQGFVIRSLMSDGDYHEVQNDLSEMTVRYRVASPQRKT
                     DYTAVRAGNLILIQGELAGRPLSRVLRIDARPWFETVEVSLSRLAVEGSSQPFVFWIV
                     HPWEARAYLMQAKVEPQQLIAVADANELATPIRMSQAGFFAFLWSALYWYRWPDGLFV
                     RYNGVRGLPGTPPTVVELIAEQNVPR"
     gene            complement(641..2332)
                     /locus_tag="VFH83_08745"
     CDS             complement(641..2332)
                     /locus_tag="VFH83_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HET6486496.1"
                     /translation="MTKGYQETNQITGQRRWIALAAVMVTMFFSSLDQTVVSTAMPTI
                     IGELNGLSLYAWLFTAYMITSAITVPLYGKLSDIYGRKPFYAFGLIGFMVGSALAGLS
                     HTMAWLIAARAIQGIGGGAMLSMPRATIGDIFNPKERGRWMGLIFATFGLASIIGPFF
                     GGWITDSIGWRWIFYINLPVAAVALAAVFYALPSVRTATRHHIDWFGSATLMLSLVPI
                     LIAFTWAGSKYPWGSPEILGLFAAGLVFLVVFLMVERRAKEPVLNPKLFRNSIFTTTV
                     AMSLFSSVGMFGGLIFLPMFVQGVVGLSASNSGAVLTPMMLSLILGSTVGGLIISATG
                     KYKLQAALGALIMIGGTVLLALMDVHTTWLIVVRNMLVMGLGIGAVMPLANIVVQNAF
                     PYSQMGVVNSTQQFVTSLGGIIAAPIFGTVLSNTFNASLRQNLPSQLATAIDKMPDAI
                     KSAFSNPQALIDSRAQAALQAKFAAFGPAGQSLYADFIKAVKLSLTQGIGKVYLFGII
                     FAVLTLATIALLKEIPLKKDEFYKEVNVEEDLGQESSPRVEAAIGAATAASEVET"
     gene            complement(2938..3363)
                     /locus_tag="VFH83_08750"
     CDS             complement(2938..3363)
                     /locus_tag="VFH83_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486497.1"
                     /translation="MEKVLPADLEEAAELLRAKIRVKERLRVSAGLSGREDPWQVRSR
                     KSMSILETGKNRVERYILEVARGMNREDSVGTVLWWPTPSASVHGGGVSGGEILRIYN
                     KGTGWRSMEIARPDLDGCLDHPELLTKYESEVSQILVEL"
BASE COUNT          657 a         1084 c         1099 g          663 t
ORIGIN      
        1 atccaggagg agcaccagtc tcaagggttc gtgatccgtt ctctgatgag cgacggagac
       61 taccacgagg tgcagaatga tctctcggag atgacggtgc ggtatcgcgt ggccagtcct
      121 cagcgaaaga cggattacac ggcggtgcgg gcgggcaacc tgatcctgat tcagggggag
      181 ctggcgggaa ggcccctgtc gcgcgtgctc agaatcgatg ctcgcccctg gtttgaaacc
      241 gtggaagtct ccctgagccg gctcgccgtg gaagggtctt cgcagccatt cgttttctgg
      301 atcgtgcatc catgggaggc cagggcgtat ctcatgcagg caaaggtcga gccgcagcag
      361 ctcatcgcgg tggccgacgc aaatgagctg gccaccccca ttcggatgag tcaggcaggc
      421 ttcttcgcct ttctctggag cgcgctctac tggtatcgct ggccggacgg actgttcgtg
      481 cggtacaacg gcgtacgggg cctgcccggt accccgccga ccgtcgtgga gttgatcgcg
      541 gagcagaacg tcccgcggtg agggctaccg gtcttgcggg aggtccaggg gttctccaaa
      601 cgggacccga accgtggacc tcccgctgag ccgccggaac ctaggtttcc acctccgacg
      661 ccgccgtcgc ggcgccgata gcagcttcga cccgcggaga gctttcctgg cccaggtctt
      721 cctctacgtt cacttccttg tagaactcat ccttcttgag ggggatctcc ttgagcagag
      781 caatggtcgc cagagtgagg accgcgaaga tgatcccgaa gaggtagacc ttcccgatcc
      841 cctgggtcag ggagagcttg accgccttga tgaagtccgc atagagagac tgccccgcgg
      901 ggccgaaagc cgcgaacttg gcttggagcg ccgcttgggc ccgggagtcg atcagcgcct
      961 gaggattcga gaacgcgctc ttgatcgcgt ccggcatctt gtcgatggcg gtggccagtt
     1021 gcgacggaag attctgccgc agcgacgcgt tgaacgtatt ggacaggacc gtcccgaaga
     1081 tcggcgcggc aatgatgcct cccagggacg tcacgaactg ctgcgtggag ttgacgactc
     1141 ccatctggga ataggggaag gcgttctgaa ccacgatgtt ggccaggggc atgacggccc
     1201 cgatccccag ccccatcacg agcatgttgc gcaccacgat cagccaggtg gtatggacgt
     1261 ccatgagcgc gagcaggacg gtcccgccga tcatgatgag ggccccgagc gccgcttgaa
     1321 gcttgtactt gcccgtcgcg ctgatgatca gcccgccgac ggtgctgccc aggatgaggc
     1381 tcagcatcat gggcgtgagc accgcaccgg agttcgatgc ggagagaccc acgacgcctt
     1441 gcacgaacat cggcaggaag atgagccccc cgaacatgcc gacggaggag aaaaggctca
     1501 tcgccacggt ggtcgtgaag atcgagttcc tgaagagctt ggggttcagg accggctctt
     1561 ttgcccgccg ctccaccatg agaaagacga ccaggaacac cagcccggcg gcaaacagcc
     1621 caaggatctc cgggctgccc cacgggtact tgctgcccgc ccaggtgaag gcgatcagga
     1681 tcggcacgag actcagcatt agggtggcgc ttccgaacca gtcgatgtgg tggcgtgtcg
     1741 cggtccgaac actcggcaag gcatagaaga ccgccgcaag agccacggct gccaccggca
     1801 ggttgatgta gaagatccag cgccagccga tgctgtccgt gatccatccg ccaaagaacg
     1861 gaccgatgat gctcgccagg ccgaaggtgg cgaagatcag ccccatccat cggccccgct
     1921 ccttggggtt gaagatgtca ccgatggtcg cgcggggcat gctcaacatg gcgcccccgc
     1981 cgatcccctg gatagcccgg gcggcgatca gccacgccat ggtgtgggag agacccgcaa
     2041 gcgcggaccc caccatgaaa ccgatcagcc cgaaggcgta aaagggcttg cggccgtaga
     2101 tgtcggagag tttgccgtac agagggaccg tgatggcgga ggtgatcatg taggcggtga
     2161 acagccacgc gtagaggctc aacccgttga gctcgccgat gatcgtcggc atggcggtag
     2221 atacgaccgt ctggtcgaga gaggagaaaa acatcgtcac catcacggcc gcgagggcaa
     2281 tccatctcct ctggcccgtg atctggttcg tttcctggta ccccttggtc atgcatcgct
     2341 ccttcgaata gttaagtgac cttaaatata tagagggcta atcaaatagg ccaatgcggc
     2401 acgatcgtca accgggtcga aacgcgcctt tccgtgcggt accgcccgtt ggagcggtcc
     2461 ggcgcgaaac ggattcaggc cgaccgtgtg tgttggccgc ccgtctcttt tccgaagggg
     2521 tctatctcag gggatggcct tgagcgtctc tggggtgcca gcggcggccg tgttctcatc
     2581 cgggtattcg tcgttcatga cgaccaggca gcgcggcgac cttttcgtac cttccgttta
     2641 tgatcctggc ggattcctcg tccacgtatt gctgggtagc ctccgagtat tcccgcagcg
     2701 tcatggcctt ggcgtctccc atcagctggc cctgcctccg ggcgggcagg aacgtgttgc
     2761 ggaaccaatc gtccgaaggc tcttccggat gcggaccggg ctctgcgcgc gatcggtcac
     2821 ctgcgtgcgg ggttgagcgg cgcgggagcc caacgccggg aagaatactc tcgagagccg
     2881 cgcgcatccg tacggcagca cacatagtct gagcgaccga gagcctagga gagtccgcta
     2941 cagctccacg aggatctggc tgacctcgct ctcgtacttc gtgagcagct cggggtggtc
     3001 caggcagccg tccaagtccg gccgggcaat ctccattgat ctccagccgg ttcctttgtt
     3061 gtagatcctg agaatctccc cgccggacac tcctccccca tggacgctgg ctgatggggt
     3121 aggccaccag aggaccgtac ccaccgagtc ctcccggttc atgccccgcg ccacttccag
     3181 gatataccgc tcgactctgt tcttcccggt ctcaaggatg ctcatgctct ttctactcct
     3241 tacctgccac ggatcttctc ttcctgaaag gccagccgac actctcaatc gctccttcac
     3301 ccggatcttg gcacggagga gctcggctgc ctcctccagg tcggccggca gcaccttctc
     3361 cacctagggc ttccctttgg agccgttggt ggccaacggt agaataccgg ccatctcacc
     3421 gatgacgttg atgagctgag agttgccggg aaccacgatc ttggcgttgg aggacatggc
     3481 tttctcgaca gcctccagcc tcc
//
LOCUS       DASRZS010000362         3863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4434331, whole genome shotgun sequence.
ACCESSION   DASRZS010000362 DASRZS010000000
VERSION     DASRZS010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4434331"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VFH83_08755"
     CDS             complement(<1..771)
                     /locus_tag="VFH83_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6486498.1"
                     /translation="MDCRRNRGLGAIAVLFGVLGVMAVGPAPVAAYGAGRTETHANSR
                     LEIFSWWTAGGEAEGLAAMFELFAEKHPSVQLINAAVAGGAGTNAKAVLDTRMRGGNP
                     PDSFQVHAGHELIDAWVVPGKMEPITFIFRDNGWLNAFPKDLIEIISYKGEIYSVPVN
                     IHRSNVLWYNKRIFAENHLAAPRTFGDLLKACEALAGRGVTPLALGDNGIWTSVHLLE
                     SVLLGTMGPDRYKGLWTGATPWDGPEVRSALARFAALMR"
     gene            complement(761..2107)
                     /locus_tag="VFH83_08760"
     CDS             complement(761..2107)
                     /locus_tag="VFH83_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6486499.1"
                     /translation="MTILIVDDEPGLRNGLSKILALKGYRTLEAGTAAEARKLLSGAE
                     VHLVLLDLKLGAEDGLALLKQLRAEEPTIPVIIITGHGDIYSAVQCMQSGATNYIPKP
                     IDHDLLLSILDKEREALRDRQSNLAFRESLRASARTRLAPSSSPEMKEIEKVIGKIRD
                     SDVPVLLLGETGTGKEVVARTIHYSGAYADRPFVGLNCASLNENLLESELFGHEKGAF
                     SGAVARKIGRFELAGNGTLFLDEIGDMSLAMQAKLLRVLQERTLERVGGTKPVSVYCR
                     LIAATNKDLTALRGAGAFREDLYYRLATVTLKLPPLRERARDIPALVRLFVEEANATY
                     GRAVHRIPDSLMRRLAAHPWPGNIRQLRNVISNAVILSDGEEIGDLELGEPAIPGEEI
                     PMEGGLRTTLARHSTELERRIIRSALEANGGNVSRTAILLGISRKTLYDKMRRHGL"
     gene            complement(2088..>3863)
                     /locus_tag="VFH83_08765"
     CDS             complement(2088..>3863)
                     /locus_tag="VFH83_08765"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6486500.1"
                     /translation="ADERAGVPPTDTLPAAILDPRAFRFPTNLFVTAADRGGTLRLYL
                     VTGTAIVRTGKPTRHLYLVTDVDRSLVEEILEKTGTEIAFYLGNVPLVYASDLQPFDT
                     AGPLRVRTMRFGDRPYSVYARPLSADLRDRLYLVSMRSTLEEALYVRSVLISYLTAFL
                     VTLAASLFLAAGITSLTVNPFTRLAAWLHRYLDSGEVGPLTIRSRDETGFLAGAFYSM
                     VSTLIEDKRTASDQLEQIRQLHASNERIMNGIQAAIVVTGDDGAIEFCNRYFGELVGE
                     KDLSALHGQGFRELMARWFTLRDGRPVADAIRLDGDCVVEGLKRERPGEDPLHFTAKL
                     SPMGLSGSRGGFLIVLEDVTASERFWAGMTIADRVTSLGILSAGVAHEINNPLGSILS
                     HVSYLKAVEKRRGKLDSLEWIESETNRIAAIIKRMRAYSAPEGAGARSADLNLVASQT
                     VELLRFTLEKRQLRLSLELAEEVGQVLCPPDELKQVVLNILLNACEASADGGSIRVGT
                     HLNGNGTAVLAIADQGVGIDPARLKNIFEPFYTTKSASQGNGLGLSICYAIVKRAGGD
                     IRVASAPGVGTEVEVTLGVHDHPDR"
BASE COUNT          618 a         1310 c         1297 g          638 t
ORIGIN      
        1 gcgcatgagc gcggcaaacc gggcgagcgc cgagcgcacc tcgggcccgt cccacggcgt
       61 ggcccccgtc cacaggccct tgtagcgatc cggccccatg gtcccgagga gcacgctctc
      121 caggagatgc acggaggtcc agatcccgtt gtcccccaga gcgagcggcg tcacgccgcg
      181 accggccagg gcctcgcagg ccttcagaag gtccccgaag gtcctgggcg cggccaggtg
      241 gttctcggca aagatgcgtt tgttgtacca cagcacgttc gagcggtgga tgttcacggg
      301 aacgctgtag atctcccctt tgtaggagat gatctcgatg aggtccttgg ggaaagcgtt
      361 cagccatccg ttgtcccgga agatgaaggt gataggctcc atcttgcccg gcaccaccca
      421 cgcgtcgatc agctcgtggc cggcgtggac ctggaaggag tccggcgggt tgccgccccg
      481 catccgcgta tccaggaccg ccttggcgtt ggtgcccgcg ccgccggcca cggccgcgtt
      541 gatgagctgg acggacgggt gcttctcggc gaagagctcg aacatcgcgg ccagcccctc
      601 ggcctcccca cccgcggtcc accaggagaa gatctccaga cgggagttgg cgtgggtctc
      661 cgtccggccg gccccgtagg cggccacggg cgccggaccc accgccatca cgcccagcac
      721 gccgaacagg acggcgatgg ccccgaggcc gcggtttctt ctacagtcca tggcgcctca
      781 tcttgtcgta cagagtcttg cggctgatcc ccagcaggat ggccgtgcgg gacacgttgc
      841 cgccgttcgc ctccaaggcc gagcggatga tcctccgctc aagctcggtg gagtgccggg
      901 ccaaagtcgt gcgcaggccc ccctccatcg ggatctcctc ccccgggatc gccggctcgc
      961 ccagctccag gtcgccgatc tcctccccgt cactgaggat caccgcgttg ctgatcacgt
     1021 ttcgaagctg gcggatgttg cctggccagg ggtgggccgc caggcggcgc atcagggaat
     1081 ccgggatgcg gtggacggcg cggccgtaag tggcgttggc ctcttccacg aacagacgga
     1141 ccagggcggg gatgtcccgc gcccgctccc gaagcggcgg gagcttgagc gtcacggtgg
     1201 ccaaccggta gtacaggtcc tcgcgaaacg ccccggcccc ccgcaatgcc gtgaggtcct
     1261 tgttcgtggc cgcgatcagc cggcaataca cggacaccgg cttggtcccg cccacgcgct
     1321 cgagggtccg ctcctgcagc acccgcagca gcttggcctg catggcaagg ctcatgtcgc
     1381 cgatttcgtc cagaaagagc gtgccgttgc ccgcaagctc gaagcggccg attttgcggg
     1441 ccaccgcgcc ggagaacgcc cccttctcgt gcccgaagag ctcgctctcc aggaggttct
     1501 cgttgagaga cgcgcagttg agacccacga agggccgatc cgcgtaggca cccgagtagt
     1561 ggatggtccg ggcaaccacc tccttccccg tgccggtctc ccccaggagc aggacgggca
     1621 catcggagtc acggatcttt ccgatgacct tctcgatctc cttcatttcc ggagaggagc
     1681 tgggggccag ccgggtccgc gcgctggcgc gcagggattc gcggaaggca aggttgctct
     1741 ggcggtcccg cagcgcttcc cgttccttgt ccagaatcga gagcagaagg tcgtgatcga
     1801 tcggcttggg gatgtagttg gtggccccgg actgcatgca ctgcacggcg ctgtagatgt
     1861 ccccgtggcc ggtgatgatg atcaccggga tggtgggctc ctcggcgcgc agctgcttca
     1921 gcagcgccag cccgtcctcc gctcccagct tgaggtccag cagcaccagg tggacctcgg
     1981 cgccggagag gagcttacgg gcttccgcgg cagtgccggc ctccagggtc cgatacccct
     2041 tgagcgcgag gatcttcgac agaccgtttc ggagccccgg ctcgtcgtca acgatcagga
     2101 tggtcatgta caccgagggt cacctccacc tccgtgccca cgcccggggc gctcgccacc
     2161 cggatgtctc ccccggcccg tttgacgatc gcgtagcaga tcgagagacc cagaccgttg
     2221 ccctggctcg cgctcttggt ggtgtagaag ggctcgaaga tgtttttcag ccgcgcggga
     2281 tcgatgccta ccccctggtc cgcgatggcg agcaccgcgg tcccgtttcc gttgaggtgg
     2341 gtgcccacgc gaatggatcc cccgtccgcg ctggcctcgc acgcattgag gaggatgttg
     2401 agaaccacct gcttcagctc gtccggcggg cacagcacct gtcccacctc ctcggccagc
     2461 tccagggaga gacggagctg acgcttctcc agggtgaagc gcagcagctc caccgtctgg
     2521 ctggccacca gattcaggtc cgcgctgcga gccccggccc cctcgggagc agaatacgcg
     2581 cgcatccgct tgatgatggc ggcgatccgg ttggtctcgc tctcgatcca ctccagggaa
     2641 tccagcttgc cgcgccgctt ctccacggct ttcaagtagc tcacgtggga caggatggag
     2701 cccaggggat tgttgatctc gtgcgccacg ccggcggaga ggatgcccag ggacgtcacg
     2761 cggtccgcga tggtcatccc ggcccagaac cgctcggaag cggtcacatc ctcaagcacg
     2821 atcaggaagc cgcctcgact ccccgagaga cccatggggg agagcttggc ggtgaaatgg
     2881 agagggtcct cccccggccg ctcccgcttc agcccctcca ccacgcagtc gccgtccagc
     2941 cggatggcat ccgctacggg ccgaccgtcc cgaagcgtga accagcgggc catgagctcg
     3001 cggaagccct ggccgtgcag cgccgagaga tccttctctc caacgagctc gccgaagtag
     3061 cggttgcaga actcgatggc tccgtcatcc cccgtcacca cgatggcggc ctggatcccg
     3121 ttcatgatcc gctcgttgga ggcatggagc tggcggatct gctcgagctg gtcgctcgcc
     3181 gttcgcttgt cctcgatcag cgtgctcacc atggagtaga aggcgccggc aaggaacccg
     3241 gtctcgtcgc gggagcgaat ggtgagcggc cccacctccc cgctgtccag gtaccggtgc
     3301 agccacgccg ccaggcgcgt gaagggattg acggtgaggc tcgtgatccc cgcggccagg
     3361 aacagggagg ccgccagggt gacgaggaac gcggtcaggt agctgatcag caccgagcgg
     3421 acgtacagcg cctcctccaa cgtggagcgc attgacacca ggtacaggcg gtcccggagg
     3481 tccgcggaca acgggcgcgc gtaaacgctg taggggcgat ctccgaaccg catggtccgc
     3541 acccgcagcg ggccggccgt gtcgaacggc tgaaggtcgg aggcgtagac cagagggacg
     3601 ttgcccaggt agaaggcgat ctcggtcccc gtcttttcga ggatctcctc caccaggctt
     3661 cggtccacgt ccgtgaccag gtagaggtgg cgcgtgggct tccccgtgcg gacgatggcg
     3721 gtgccggtga ccaggtacag cctcagggtc ccgccgcggt ccgcggcggt tacgaagagg
     3781 ttggtcggga agcggaacgc tcgagggtcc aggatggcgg ccggcaaggt gtccgtgggg
     3841 gggacgccgg cgcgctcgtc tgc
//
LOCUS       DASRZS010000363         5579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1357489, whole genome shotgun sequence.
ACCESSION   DASRZS010000363 DASRZS010000000
VERSION     DASRZS010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357489"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..1492)
                     /locus_tag="VFH83_08770"
     CDS             complement(254..1492)
                     /locus_tag="VFH83_08770"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HET6486501.1"
                     /translation="MFYADPSLLAEGVSSYRRSSWDRSGRNADFFLLQPGQTVTLLEE
                     SGPGKICHFYWATAFASLFHYRQLVLRAYWDGETTPSIEAPIGDLFCIPHCTPVPIHS
                     LAVVINPGSPDLLTLGANLYLPMPFASSARVTVDYDPIPDHPADPIYFWYHINLERHP
                     RPPADNVGRFHAQWRRENLTQPVDPKFRNVQLFDGVNLDGKENYLALRAEGHGQMVGL
                     HLQVDNVGGGWYGEGDDMVFVDGQPGQQWPPTYHGTGTEEIFGGGACPDKPYAGPDTG
                     FHMVENTDFAGKHAMYRWYLADPIRFERSLVWTVEHGHANSFENDYSSVAFWYQQEPH
                     APFPALPDVKARIPRFPDVVWQADALRGRAKEEMDRLMKQGLPQQELQRVFAQGRAAR
                     AALAAGRLEEAISLYKSMKG"
     gene            complement(1511..2644)
                     /locus_tag="VFH83_08775"
     CDS             complement(1511..2644)
                     /locus_tag="VFH83_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6486502.1"
                     /translation="MSSYLVPLQHPRPDIEHFLRAVRGEIAPKKPPMVEYLVDNALMQ
                     PILEDLLHRPWVDTSDKTEYMGGQMDLSREARHSVDAWLDNQIEFWYRMGYDFVRVEV
                     SLPLPAVSHVVADTARGNEEHARAWQGLGTGPIQSWKDFERYPWPKITDDNFYIHRYI
                     CSHLPDGLGFISCHAGGVYEHVSRLMGYENLCLKLYDQPDLVRAVTDRLGELILEYNR
                     RLLELDRLSVIFQGEDFGFNTQTLLPPQVIRDYFLPWHARYASQIHARGLPYFMHSCG
                     AVDELMDDLIDVVKIDGKHSFQDNVVPVTEAKKRWGKRIGILGGIDVHKLTTLTEEEL
                     RAYVRRVIDACADGGRFAVGAGNSIPSYIPMGNYLTLLDESLR"
     gene            2772..4130
                     /locus_tag="VFH83_08780"
     CDS             2772..4130
                     /locus_tag="VFH83_08780"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HET6486503.1"
                     /translation="MKTLPTRMAVLVSAWLLAAAVSAMAQTTPSTPDRLLDSLFLLRD
                     TKTVRISSWDRSGGNYDFTWIAPGETQTIANIQGAGIIRRFYVAPYAMDRMRYRKVVL
                     RMYWDGEQQPSVEVPLGDFFGSGLGTLRYFHSVAMDVNPGFSGYDFDAMVCYLPMPFA
                     KGARITIENDGGVKDFLLWYHIEYEQLPDGGLPANAGRLFAQWRRVARTPVRDGIPKN
                     TQLGANPTRNTTGADNYVALDAEGHGSYIGLFLTVDNIVGGWYGEGDDMIFIDGVSWP
                     PAYPGTGHEEIFNAGACPDTEFAGLYTGFYLIENLHGPWGGKNQMYHFYVNEPVAFQK
                     SIRVTIEHGHNNNFENDYTSTAFWYQQEPHKDFPPLPAAAERLPAWPPEVAAAMDKEY
                     DLRRLVSGGGVRMAKDDSVQWDQLEAARNREFRALAFTDYIRDVDAAGALVDKYRPPA
                     GK"
     gene            complement(4164..5234)
                     /locus_tag="VFH83_08785"
     CDS             complement(4164..5234)
                     /locus_tag="VFH83_08785"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6486504.1"
                     /translation="MATAPGWQEFRVSNDALLDAEELRRRMAEEGYLFFRRLVNPDLM
                     LSLRREVMGILQNHGWLCVGTDPMDGIVDLSKRCTEGDPPYAAPYHDVYKLESFHRLP
                     HCPELVSTVERIMGTRTIPVPGHKARIWFPKFKEHTTPTHQDFVHYQGSLQAITCWTP
                     VGDCPIDLGPLAVLPKSHTVKKVLPHHFSLGAGGLIIRVEDEVQKFPVLDVPWHSTNF
                     EVGDVLFFPALTIHRALPNVTEDRLRVSLDNRYQREGDNIAAHMLTPHMSDMYPITWD
                     EIYKDWKADDLKFYWRRMRHRPIPKYNGYSEIGWAEATELARHRDKHALLAMKRVVTL
                     HPTSEQAETARKVLDEVGAAQQ"
     gene            complement(5329..>5579)
                     /locus_tag="VFH83_08790"
     CDS             complement(5329..>5579)
                     /locus_tag="VFH83_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6486505.1"
                     /translation="CNSMIVKRAGSNVGGTGATYRWGLDDPTGIQRLEPKLELIQELK
                     PSELVAFSRFPLWWRALFRLLELNSTLRRMERVVVYGF"
BASE COUNT          980 a         1762 c         1840 g          997 t
ORIGIN      
        1 caggatggaa aggtatcccg ccagcgcctc ccggcggcgc atcggggagg aatactcgga
       61 gcgtcggatc ctggtgggtt gaaggcttgt ctgggcgagc gtgccgtttg ctgaagccac
      121 cctgcctcct ttgaggagcg cccgtgcccg accgcgcgcc ggtgcggcac gaaagcctta
      181 gtgcgtaatc tagtgaaggg gccccgcgaa gtcaaggaaa aagggctccc gaccgcgggc
      241 tgctcggcga cgcctatccc ttcatgctct tgtagagcga gatcgcctcc tccagccgac
      301 cggcggcaag cgcggcgcgc gcggccctgc cctgggcgaa gacccgctga agctcctgct
      361 gcgggaggcc ctgcttcatc aggcgatcca tctcctcttt ggcccgcccg cgcagtgcat
      421 ccgcctgcca gaccacgtcc gggaagcggg ggatccgcgc cttcacgtcc ggcagcgccg
      481 ggaacggcgc atgcggctcc tgctggtacc agaacgccac gctggagtag tcgttctcga
      541 agctgttggc gtggccgtgc tccacggtcc agaccagcga tcgctcgaag cggatggggt
      601 ccgcgagata ccagcggtac atcgcgtgct tgccggcgaa atccgtgttc tccaccatgt
      661 ggaaccccgt gtcgggcccc gcgtagggtt tgtccggaca ggcgccgccg ccgaagatct
      721 cctccgttcc cgtgccgtga tacgtgggcg gccactgctg gcccggctgg ccgtcgacaa
      781 agaccatgtc gtctccttcg ccgtaccaac cgcccccgac gttgtccacc tggaggtgca
      841 gacccaccat ctggccgtgc ccctccgcgc ggagcgccag gtagttctcc ttgccgtcca
      901 ggttgacgcc gtcgaagagc tgcacgtttc gaaacttggg atccaccggt tgcgtcaggt
      961 tctcccgacg ccactgcgcg tggaatctgc cgacgttgtc cgccggcggg cggggatggc
     1021 gctccaggtt gatgtggtac cagaagtaaa tcgggtcggc cggatgatcc gggatgggat
     1081 cgtagtccac ggtgactctg gcggaggagg cgaacggcat gggcagatag agattcgcgc
     1141 ccagcgtgag gaggtccgga ctacccgggt tgatcaccac ggcaagggag tgaatcggaa
     1201 cgggcgtgca atgcggtatg cagaaaaggt ccccgatggg ggcctcgatg ctcggtgtgg
     1261 tctcgccatc ccagtaggca cggagcacga gctgccggta gtggaagagg ctggcaaagg
     1321 ccgtagccca gtagaagtga cagatcttgc cgggaccgga ttcctccaac agcgttacgg
     1381 tctgcccggg ctggagcagg aagaagtccg cgttgcgccc cgagcggtcc cacgaggagc
     1441 ggcgatagct gctgacgcct tccgcgagaa gcgatggatc cgcgtagaac acggtgacct
     1501 ccttggtcgt ctaccgaagg gattcgtcca ggagcgtgag gtagttcccc atcgggatgt
     1561 agctggggat ggagttgccc gcgcccacgg cgaatcggcc gccatcggcg caggcatcga
     1621 tgacgcgccg aacgtacgcg cgaagctcct cctccgtgag cgtcgtcagc ttgtgcacat
     1681 cgatgccgcc caggatgccg atgcgcttgc cccagcgctt cttcgcctcc gtcacgggaa
     1741 ccacgttgtc ctggaaggaa tgcttgccat cgatcttgac cacgtcgatc aggtcgtcca
     1801 tgagctcgtc caccgcgccg cagctatgca tgaagtacgg cagccctcgg gcatggatct
     1861 ggctggcata cctggcgtgc cacggcagga agtagtcgcg gatgacctgg ggcgggagaa
     1921 gggtctgggt gttgaagccg aagtcctctc cctgaaagat cacggacagc cggtcgagct
     1981 ccaggagccg ccggttgtac tccaggataa gctcgcccaa ccggtcggtc accgctctca
     2041 ccaggtcggg ctggtcgtag agcttgaggc agaggttctc gtatcccatg agccgggaca
     2101 cgtgctcgta gacgccaccg gcgtgacagc tgatgaaccc caagccgtcc gggaggtgag
     2161 agcagatgta gcgatggatg tagaagttgt cgtccgtgat cttcggccac gggtaccgct
     2221 cgaagtcctt ccagctctgg atggggccgg tgcccagtcc ctgccaggcg cgcgcgtgct
     2281 cctcgttccc ccgggcggtg tccgccacca cgtgcgacac ggcaggcagg ggcaggctca
     2341 cctcgacgcg cacgaagtcg tagcccatgc gataccagaa ctcgatctgg ttatccagcc
     2401 acgcgtccac gctgtgccga gcctcccggg acaggtccat ctgaccgccc atatactcgg
     2461 tcttgtccga cgtatcgacc cacgggcgat ggagcaggtc ctccaagatc ggctgcatga
     2521 gagcgttgtc tacgaggtac tcgaccatag ggggcttctt gggggcgatc tcaccgcgca
     2581 cggcgcgcag gaagtgttcg atgtcgggcc gggggtgctg aaggggaacc aggtagctgc
     2641 tcatctcgcc tcctgttcgc ctaggtggtg ggcgccaacc aacgtaccac cgcggcgtcc
     2701 ggaagtaaag ttgccgcacg ccgtggcgcc gggcgcggcg gctcggttat actgcacggg
     2761 gaggatggca aatgaagacg ctcccgactc ggatggcggt tctggtttcc gcgtggctgc
     2821 ttgccgcggc ggtttcggcg atggcccaaa ccaccccttc gacaccggac cggctcctgg
     2881 acagtctgtt cctgctcagg gatacgaaga cggtgcggat ctcctcctgg gaccgcagtg
     2941 gcggcaacta cgacttcacc tggatcgccc ctggagagac ccagacgatt gccaacatcc
     3001 agggcgccgg catcatccgc cgcttctacg tggccccgta tgcgatggac cgaatgcgct
     3061 accgcaaggt cgtgctgcgg atgtactggg acggggagca gcaacccagc gtggaagtgc
     3121 ccctgggaga cttctttgga tccgggttgg ggacgcttcg gtatttccat tccgtggcta
     3181 tggacgtcaa ccccggtttc agcggctacg acttcgatgc catggtctgc taccttccga
     3241 tgcccttcgc caagggggcc cggatcacca tagagaacga cggaggcgtc aaggacttcc
     3301 tcctctggta ccacatcgag tacgagcagc taccagacgg cggactgccc gccaacgcgg
     3361 gtcggctctt cgcccagtgg aggcgggtgg ccaggacccc ggtccgggat ggaatcccca
     3421 agaacacgca gctcggcgcc aacccgactc ggaacacgac cggcgcggac aactacgtgg
     3481 ctctcgacgc ggaggggcac ggaagctaca tcggcctctt ccttaccgtg gacaacatcg
     3541 tcggcggctg gtatggcgag ggcgatgaca tgatcttcat cgacggagtc tcgtggcctc
     3601 ccgcctaccc cggaacgggt cacgaggaga tcttcaatgc cggggcctgt ccggacacgg
     3661 agtttgccgg cctgtacacc gggttctacc tcatcgagaa ccttcacggg ccgtggggcg
     3721 ggaagaacca gatgtaccac ttctacgtga acgaaccggt ggcgttccag aagtccatcc
     3781 gcgtcaccat cgagcacggc cacaacaaca acttcgagaa cgattacacc tccacggcgt
     3841 tctggtatca acaggagcca cacaaggact tccctcccct ccccgcggcc gccgagaggc
     3901 ttcccgcgtg gccaccggag gtggcggcgg ccatggacaa ggaatacgac cttcggcgcc
     3961 tggtgtcggg tggcggcgtc cgcatggcca aggatgacag cgtccagtgg gatcagctcg
     4021 aggccgcgcg caatcgggag tttcgggcgc tcgcctttac ggactatatc cgcgacgtcg
     4081 atgcggcggg tgcgctcgta gacaagtacc ggccgccagc gggaaagtga tcggccgcag
     4141 ggcggagcaa gcagggggcg cggctactgc tgcgccgccc ctacctcgtc caggaccttg
     4201 cgcgccgtct ccgcctgctc cgaagtggga tggagggtga cgacgcgctt catcgcaaga
     4261 agcgcgtgct tgtcccggtg cctggccagc tcggtggcct ctgcccagcc gatctccgag
     4321 tagccgttgt acttggggat cggtcgatgc ctcatccgcc gccagtagaa cttcaggtcg
     4381 tcggccttcc agtccttgta gatctcatcc caggtgatgg ggtacatgtc gctcatgtga
     4441 ggcgtgagca tgtgcgccgc gatgttgtcg ccctcacgct ggtaacggtt gtccagggaa
     4501 acccgcagcc ggtcttccgt gacgttgggc agggcccggt ggatggtcaa cgccgggaag
     4561 aacaggacgt ctccgacctc gaagttggtg gagtgccagg gcacgtccag gaccgggaac
     4621 ttctggactt cgtcctccac ccggatgatc aggccaccgg ctccgaggga gaagtgatgc
     4681 ggcaggacct tcttcacggt gtgggacttg ggaaggaccg ccaggggccc gaggtcgatg
     4741 gggcagtctc ctaccggggt ccagcaggtg atggcctgga ggcttccctg gtagtggacg
     4801 aaatcctggt gcgtgggggt ggtgtgctcc ttgaacttgg ggaaccagat ccgcgccttg
     4861 tgaccgggca cggggatggt ccgggtgccc atgatcctct ctacggtaga gaccagctcc
     4921 gggcagtgcg gcaggcggtg gaaggattcg agcttgtaca cgtcgtggta gggagccgca
     4981 tagggcgggt cgccctcggt gcagcgcttc gaaagatcca cgatgccgtc catggggtcg
     5041 gtcccgacgc agagccagcc gtggttctgg aggatcccca tgacctcccg gcggagggat
     5101 agcatcaggt ccggattcac cagccttcga aagaagagat aaccctcttc cgccatccgg
     5161 cggcgcagct cctcggcatc gagaagcgcg tcgttcgaaa ccctgaactc ctgccaacct
     5221 ggggccgtgg ccatcgcctt cctcctgtcc ggttcactcc tttcccgaag gtactgaggc
     5281 ttcctgagga agtcacgcgg aacctctcgt caactccgag cgtgcgtctc aaaacccgta
     5341 gacgaccacg cgttccatcc tccgcagggt ggagttgagc tccagcagtc ggaaaagcgc
     5401 tcgccaccac aacgggaagc gggagaacgc gaccagctcg ctcggtttga gctcctggat
     5461 cagctcgagc ttcggctcga gccgctggat gccggtcggg tcgtcaaggc cccaacggta
     5521 cgttgccccc gtgcctccca cgtttgaccc ggctcgcttc acaatcatgg agttgcaga
//
LOCUS       DASRZS010000364         6421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4435164, whole genome shotgun sequence.
ACCESSION   DASRZS010000364 DASRZS010000000
VERSION     DASRZS010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4435164"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFH83_08795"
     CDS             <1..199
                     /locus_tag="VFH83_08795"
                     /inference="COORDINATES: protein motif:HMM:NF018504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486506.1"
                     /translation="EAIEKKLIADLKNIVSLTLADKSRRDPKTLERKSEAISKLKERG
                     YCPVCAEALLDFVGDMLRREE"
     gene            206..1501
                     /locus_tag="VFH83_08800"
     CDS             206..1501
                     /locus_tag="VFH83_08800"
                     /inference="COORDINATES: protein motif:HMM:NF016195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HET6486507.1"
                     /translation="MVAKPDYGSSPRGRADAARHRAKVKEAIRRNLPDIISDEAIITR
                     KENRLIKVPIRGLKSYHFIHGQTGNASGGFGSGEGEKGKLIGRRPKPGRGQPGTPGDE
                     PGVDYLETEIELEELVQMMLEDLGLPNLKQKEARETLLQAGWKLDSVEKAGVWSRIDK
                     KRTVKEAILRTEILVARLMKDTGRPEAECRAALIRAGGVMERAQALLAAEHLAGSASG
                     IPEAFLESADLRFRTIREDTESESNAVIMAMMDVSGSMGTMKKYMARSFFFWMLSFLK
                     SLYRRVDIRFIAHTTEARLVDENEFFHKGESGGTRCSSAYQLAAELVDAAYPPSSWNV
                     YPFHFSDGEDWDVAETVRAAKVLLEKGVATLGYGEIQEEYGSSVLMQAFREELHLSEG
                     RTERLPYFEGDWSGSPFIGVILRAKEDLYPALRAILKPE"
     gene            1509..2999
                     /locus_tag="VFH83_08805"
     CDS             1509..2999
                     /locus_tag="VFH83_08805"
                     /inference="COORDINATES: protein motif:HMM:NF016201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HET6486508.1"
                     /translation="MPATDLARLNDADRLIQEIAHDMGLDCMPQEFDVIPDQKMLEIM
                     AYGLPVNYSHWSFGRNFDTERTRYEHGYGVPYEVVFNSDPARAYLMETNPYVIQVLVM
                     AHVYAHNDFMKNNRHFGLSRRDMVTSASEAAARLRQYEEDYGLEAVEKLLDAAIAIQW
                     NIDPDAAMHPETEVQERERLYGWATSVPQRGAYEDLLPPKPEASSVEKRLLRLRTPPQ
                     PTVELLGYIIEHSPRPLAEWERDVLGVVREQSQYFMPYRRTKIMNEGWATFWHEKIMQ
                     RLFALKFLDAEAHGIYNLYNARVKAHNPRSINPYLLGHAMFTDIEERWDKGRFGKEYE
                     ENVMALERDAWDKKLGLGRKKIYDVRRTYMDWFFLDEFLTRELMEKLKLYVYLEAERE
                     EVYETVVAETDWRAVKRLLVQNMMNWGVPRILLVDGNYQGSLQLYLQHSFEGLPLDEE
                     YCSRTLQHIYSLWGRPVLLETREPKNGSSRSKLYVADGSGIHVRTD"
     gene            complement(3038..4585)
                     /locus_tag="VFH83_08810"
     CDS             complement(3038..4585)
                     /locus_tag="VFH83_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HET6486509.1"
                     /translation="MHPSDFDALAILLHPEDEVAVARAAIPAGTRLRIGESVLVVRSD
                     IPSGHKIARHAVPAGAPVRRYGQPIGLASSRIEPGDHVHTHNLTTKGMEGTVGVAAPD
                     SWTHRSVGSGRAEPAAPSRSFLGFRRADGRVGTRNHVAVISTVNCSAAVCRFVRERFR
                     DVRADYPNVDSVTALTHEGGCGGTVGSDDHKTLMRVLGGYARHANVAGCVLVGLGCEK
                     NKVSTLVADQGLQRVPSFIIQQEGGTRRTVDAVSDAVRKLLPRANEARRTPQQASDLV
                     LATNCGGSDSWSGVTANPALGWAVDELVRWGGAALLGETTEIFGAEQLLASRAVSPQV
                     ASRLLERVRWWKEWLMRLGSTIDANPAPGNIAAGLTTVFEKSLGGISKGGTSPLVDVI
                     PYAGEVRGSGLIYMDTPGYDPVSVTGMVAGGANVVAFTTGCGSVFGSAIAPSLKLATT
                     TDLYRRMEPDMDVDCGVILDGTPLASVGAAIFDRILAVASGERTKSEEQGLGEDEFVP
                     WIRGPVT"
     gene            4634..5941
                     /gene="larA"
                     /locus_tag="VFH83_08815"
     CDS             4634..5941
                     /gene="larA"
                     /locus_tag="VFH83_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HET6486510.1"
                     /translation="MRLSLSFGRQGLPVDLPDDWDITVLAKERMPVQEDPKACIAGAL
                     RSPMGSATIAREAAGQGTACVLVCDVTRPVPNHLVLRPVIEELLGAGIDPRRITVLVA
                     TGLHRPNEGQELAAVIGDPWVFEHARVENHVARRDEDHADLGRTSHGVPVRVDRRFLD
                     AGVRIAVGLVEPHFMAGWSGGRKLVVPGIAHAETISAFHSGRMLGHPRAATAMLDGNP
                     LHEAQLQALSMMGRCLGVNVVIDENRALSFASFGDIQESHRAAVRFAEPYFLVPVSRP
                     YPVVLVSSAGYPLDATYYQTVKGICCGSTILQKGGHLFAVSECSEGVGSREFRAAQTR
                     LRDLGKERFRAEVGSRDRAQIDEWETFMLVKALDIGEAHLYTTGLSAEERRLTGAEPV
                     TDLVADLRRHVESDPERRMAVIPEGPYVAPALGRGSTPTPGEP"
     gene            6088..>6421
                     /locus_tag="VFH83_08820"
     CDS             6088..>6421
                     /locus_tag="VFH83_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6486511.1"
                     /translation="MANALKELSTALREAIAKASVFTVSIEHDPYAVSGVLIDRDQVL
                     TASHLVPDEGVRITLADGTQRDAHVLGRDPLHDLALLRLSASASASAPKQASASVGDL
                     VISLKRDPI"
BASE COUNT         1114 a         2151 c         2146 g         1010 t
ORIGIN      
        1 ggaggccatc gagaagaagc tgatcgcgga tctcaagaac atcgtttccc tcaccctggc
       61 ggacaagtcc cgccgggacc ccaagaccct ggagcgcaag agcgaagcca tctccaagct
      121 caaggagcgc gggtactgcc cggtgtgcgc ggaagcgctc ctggacttcg tgggcgacat
      181 gcttcgacgg gaagagtaac cggcgatggt cgccaaaccg gactacggct cctctccccg
      241 cggacgcgcg gacgcggccc gtcaccgggc caaggtgaag gaggccatcc gccggaacct
      301 cccggacatc atcagcgacg aggccatcat cacccgcaag gagaaccggc tcatcaaggt
      361 gcccatccgc ggattgaaga gctaccattt catccacggc cagacgggca acgcctccgg
      421 tgggttcgga agcggcgagg gggaaaaggg caagctcatc ggaaggcgtc ccaagccggg
      481 gcgaggccag ccgggcaccc cgggggacga gccgggggtg gactacctgg aaaccgagat
      541 cgagctggag gagctcgtcc agatgatgct ggaggatctg ggtcttccca acctcaagca
      601 gaaggaggcg cgggagacgc tcctgcaggc gggctggaag ctcgactccg tggagaaggc
      661 cggcgtttgg tcccggatcg acaagaagcg caccgtcaag gaagccatcc tgcgaacgga
      721 aatcctggtt gcccggctca tgaaggacac cggcaggccg gaggcggagt gtcgggcggc
      781 cctgatccgg gcgggcggag tcatggagcg cgcgcaggct ctcctggccg ccgagcacct
      841 ggcggggtcg gcgtccggga tcccggaagc gtttctggag agcgcggacc tccgctttcg
      901 gaccatccgg gaggatacgg agtccgagtc caacgcggtg atcatggcga tgatggacgt
      961 ctccggcagc atggggacca tgaagaagta catggcccgt tcgtttttct tctggatgct
     1021 ttccttcctg aagagcctgt accgcagggt ggacatccgg ttcatcgctc acaccaccga
     1081 ggccaggctg gtggacgaga acgagttctt ccacaaggga gagtccggcg gcacgcggtg
     1141 ctcctccgcc taccagcttg ccgcggagct ggtggacgcg gcctacccgc ccagctcctg
     1201 gaacgtctat cccttccact tctccgatgg cgaggactgg gacgtggcgg agacggtccg
     1261 cgcggcaaag gtcctgctgg agaagggcgt tgccaccctg ggctacggcg agatccagga
     1321 ggagtacggc tcctcggtcc tgatgcaggc gttccgcgag gagctccatc tctccgaggg
     1381 aaggaccgag cggctgccgt acttcgaagg ggactggtcc ggctcaccgt tcatcggagt
     1441 catccttcga gccaaggaag acctctaccc cgcccttcgc gccatcctca aaccggagtg
     1501 agatgcccat gccggccacc gaccttgcgc ggctgaacga tgcggaccgg ctgatccagg
     1561 agatcgccca cgacatgggg ctggactgta tgccccagga gttcgacgtg atcccggacc
     1621 agaagatgct ggagatcatg gcctacggcc tgccggtgaa ctactcccac tggagcttcg
     1681 ggaggaactt cgacacggag cgcacccgct acgagcacgg ttacggcgtc ccctacgagg
     1741 tggtcttcaa cagcgacccg gcgcgcgcct acctcatgga gaccaatccg tacgtgatcc
     1801 aggtgctcgt catggcgcac gtctacgccc acaacgactt catgaagaat aaccgccatt
     1861 tcgggctctc ccgacgggac atggtgacct ccgcctcgga ggcggcggcc cgccttcgcc
     1921 agtacgagga ggactacggt ctggaagcgg tggagaagct cctggacgcg gcgatcgcca
     1981 tccagtggaa catcgacccc gacgccgcca tgcacccgga gaccgaggtg caggagcgcg
     2041 agcggctgta cggctgggca acgtccgtcc cccagcgggg cgcgtacgag gaccttctgc
     2101 ctcccaagcc ggaggcctcc agcgtggaga agcgcctcct gcggctgcgc accccgcccc
     2161 agcctaccgt ggagctgctg ggctacatca ttgagcactc cccccggccg ctggccgaat
     2221 gggagcgcga cgtgctgggg gtggtccgcg agcagtccca gtacttcatg ccgtatcgcc
     2281 ggaccaagat catgaacgag gggtgggcga ctttctggca tgagaagatc atgcagcgcc
     2341 tgttcgcctt gaagttcctg gacgccgagg cccacggcat ctacaacctc tacaacgccc
     2401 gcgtcaaggc gcacaacccg cggtccatca acccgtacct ccttggccac gcgatgttca
     2461 ccgacatcga agagcggtgg gacaagggcc gcttcggcaa ggagtacgag gagaacgtca
     2521 tggccctgga acgggacgcg tgggacaaga agctcggtct ggggcggaag aagatctacg
     2581 acgtccgccg cacgtacatg gactggttct tcctggacga gttcctgacc cgggagctga
     2641 tggagaagct caagctgtac gtctacctgg aggcggagcg cgaagaggtc tatgagaccg
     2701 tggtcgcgga gaccgactgg agagccgtca agcgcctcct ggtccagaac atgatgaact
     2761 ggggagtgcc ccggatcctg ctggtggacg gcaactacca ggggagcctc cagctctacc
     2821 tccagcactc cttcgaaggc ctgccgctgg acgaggagta ttgctccagg accctgcagc
     2881 atatctactc gctctggggg cgccccgtac tcctggagac gcgcgagccc aagaacggct
     2941 catcccgatc caagctctac gtcgcggacg gatcgggcat ccacgttcgg accgactgac
     3001 cggcgcggcc ccgcgtatcc tcgggcccgt ccgatctcta ggtcacgggc ccccggatcc
     3061 acggcacgaa ctcgtcctcc cccagacctt gctcctcgct cttcgttcgc tccccggagg
     3121 ctaccgcaag gatacgatcg aagatggcgg caccgacgct tgccagcggc gtcccgtcca
     3181 ggatcacgcc gcagtcgacg tccatgtccg gctccatgcg gcggtacagg tcggtggtgg
     3241 tggccagctt gaggctgggc gcgatggcgg agccgaacac cgagccgcag cccgtggtga
     3301 acgccaccac gttcgcgccg ccggcgacca tccccgtcac ggagaccgga tcgtagccgg
     3361 gggtatccat gtagatcagc ccggatcctc ggacctcacc ggcatacggg atcacgtcca
     3421 ccaacggaga cgttccgccc ttggagatgc cccccaggga cttctcgaac accgtggtca
     3481 atcccgcggc gatattgccc ggagccgggt tggcgtcgat ggtggacccc aaccgcatca
     3541 accactcctt ccaccagcgg acccgttcca ggagccggga cgcgacctgc ggtgagaccg
     3601 cgcgggaggc caggagctgc tccgcgccga agatctccgt cgtttcgccg aggagcgcgg
     3661 ccccgcccca ccggaccagc tcgtccacgg cccagcccag cgcggggttg gcggtgacgc
     3721 cggaccagga gtccgagccg ccgcagttgg ttgccagcac gaggtcggat gcctgctgcg
     3781 gcgtgcgccg cgcctcgttg gcgcgaggca gcagcttgcg gaccgcgtcg ctcacggcgt
     3841 ccaccgtgcg gcgcgtgccg ccttcctgct ggatgatgaa cgagggcacc cgttgcagcc
     3901 cttgatccgc caccagggtg gagaccttgt tcttctcgca ccccaagccc acgagcacgc
     3961 agccggcaac gttggcgtgg cgggcatagc cgccgagcac acgcatgagg gtcttgtggt
     4021 cgtcgctgcc gaccgtcccg ccgcagccgc cctcgtgggt gagggcggtc acgctgtcca
     4081 cgttggggta atcggcgcgc acgtcccgga atcgttctcg aacgaaccga cagaccgcgg
     4141 cggagcagtt gacggtcgag atgaccgcca cgtgattgcg ggtccctacg cggccgtccg
     4201 cgcgacggaa tccgaggaag gagcgcgaag gcgcggccgg ctcggcccgg cccgatccca
     4261 cggaacgatg cgtccaggag tccggggccg ccactccgac ggtgccttcc atccctttcg
     4321 tggtgaggtt gtgggtgtgc acgtgatcgc cgggctcgat gcggctcgat gccagaccaa
     4381 tgggctgccc atatcgtcgg acgggcgctc cggccggcac ggcgtgtcgc gcgatcttgt
     4441 gaccgctcgg gatgtcggac cggacaacca gcacgctttc tccgatccgt agccgggtgc
     4501 ccgcggggat ggcggcgcgc gccacagcca cctcatcctc cgggtgcagc aggatggcaa
     4561 gagcatcgaa atccgatggg tgcatgacgg catgctactt ccggaagcag gtttctgcta
     4621 cagttctgtg ctgatgcgtc tctcgctgag cttcggccgg caagggctgc cggtggacct
     4681 gccggatgac tgggatatca cggtcctggc caaggagcgg atgccggtgc aggaagaccc
     4741 aaaggcctgc attgcgggcg ctctgcggtc tccgatgggc tccgccacga tcgcgcggga
     4801 ggccgcgggc cagggcacgg cctgcgtcct ggtctgcgac gtcacccgtc cggtccccaa
     4861 ccacctcgtg ctccgccccg taatcgagga gctgctggga gcggggatcg atcctcggcg
     4921 catcaccgtg ctggtagcca ccgggcttca tcgccccaac gaaggtcagg agcttgccgc
     4981 cgtcatcggg gatccgtggg tcttcgagca cgcgcgcgtg gaaaaccacg ttgcccgccg
     5041 ggacgaggat cacgcggacc tgggccgcac cagccacggg gtgccggttc gggtggatcg
     5101 ccggttcctc gatgccggcg tgcggatagc ggtagggctc gtggagcccc acttcatggc
     5161 cggctggtcc ggcgggcgga agctcgtggt gcccggcatc gcccacgcgg aaaccatctc
     5221 cgcgttccac tcgggccgca tgctgggcca tccccgggcc gcaacggcca tgctggacgg
     5281 caatcccctc cacgaagcgc agctccaggc tctctccatg atgggccgat gcctgggcgt
     5341 gaacgtggtg atcgacgaga accgcgcgct ctcgttcgcc agcttcggag acatccagga
     5401 gagccaccgc gccgccgtgc gcttcgccga gccgtacttc ctggtgcccg tgagccgtcc
     5461 ctaccccgtg gtgctggtca gctccgccgg ctatcccctg gatgccacct actaccagac
     5521 cgtgaagggc atctgctgcg gctcgaccat cctccagaag ggcggccatc tcttcgcggt
     5581 aagcgagtgc tccgaagggg tcggctcccg cgagttccgc gccgcccaga cgcggcttcg
     5641 agatctgggg aaggaacgct tccgcgcgga ggtcggatcc cgagatcgcg cgcagatcga
     5701 cgagtgggag acgttcatgc tggtcaaggc gctggacatt ggcgaggcac acctctacac
     5761 cacgggattg agcgcggagg aacggcggct caccggcgcg gagccagtga cggacctcgt
     5821 ggcggacctc cgacgccacg tggagagcga cccggagcgc cgcatggcgg tgatcccgga
     5881 gggaccctac gtggcccctg ccctgggtcg gggctccacg ccgaccccag gcgagccctg
     5941 agccggcccg gtcccaggcg tgggctcccc acctccccat tcggcgagta ccatcgtccc
     6001 acggccattc ataccttcct gtctacgggg cgcctttcgt acatccgtgt acgcggcgcc
     6061 cctcgacatc ctgtctacgg agggcatatg gctaatgcat tgaaggagct atcgaccgcg
     6121 cttcgagagg cgatcgccaa ggcgagcgtg ttcacggtga gcatcgagca cgatccctac
     6181 gcggtcagcg gggtcctgat cgatcgggat caggtgctaa cggcgagtca cctggtgccg
     6241 gacgagggtg tccgcatcac gctggcggac ggcacccagc gggacgctca cgtccttggc
     6301 agggatccgc tgcacgacct ggcgctcctg cggctcagcg ccagcgcttc cgcttccgcg
     6361 ccaaagcagg cctcggccag cgtcggggac ctggtgattt cgctcaagcg ggatcccatc
     6421 g
//
LOCUS       DASRZS010000365         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3661870, whole genome shotgun sequence.
ACCESSION   DASRZS010000365 DASRZS010000000
VERSION     DASRZS010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3661870"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..146
                     /locus_tag="VFH83_08825"
     tRNA            63..146
                     /locus_tag="VFH83_08825"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:97..99,aa:Tyr,seq:gta)
     gene            173..248
                     /locus_tag="VFH83_08830"
     tRNA            173..248
                     /locus_tag="VFH83_08830"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Gly,seq:tcc)
     gene            282..357
                     /locus_tag="VFH83_08835"
     tRNA            282..357
                     /locus_tag="VFH83_08835"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:315..317,aa:Thr,seq:ggt)
     gene            382..1572
                     /gene="tuf"
                     /locus_tag="VFH83_08840"
     CDS             382..1572
                     /gene="tuf"
                     /locus_tag="VFH83_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET6486512.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITKVLAKQGGASY
                     LAYDQIDKAPEERERGITISTAHVEYQTKNRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVYLNKVDMLDDPELRELVEMEVRDLLKK
                     YEFPGDKTPIIPGSALKALEGDTSEIGEGSIMKLMDALDTYIPTPQRQTDKPFLMPVE
                     DVFSIAGRGTVATGRVERGKIKVGEEVEIVGLRPTTKTVVTGVEMFRKLLDEGIAGDN
                     IGALLRGLKREDVERGQVLAKPGSITPHTKFKAQIYVLTKEEGGRHTPFFKGYRPQFY
                     FRTTDVTGTVKLPDNVEMVMPGDNIAIEVELITPIAMEKELRFAIREGGRTVGAGVVA
                     EVLE"
     gene            1678..1842
                     /gene="rpmG"
                     /locus_tag="VFH83_08845"
     CDS             1678..1842
                     /gene="rpmG"
                     /locus_tag="VFH83_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET6486513.1"
                     /translation="MPKGNRSIISLECPVCKRRNYSTTKNRRKSQDKLELSKFCPWCR
                     KHTDHKEGKV"
     gene            1858..1930
                     /locus_tag="VFH83_08850"
     tRNA            1858..1930
                     /locus_tag="VFH83_08850"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1891..1893,aa:Trp,seq:cca)
     gene            1948..2391
                     /gene="secE"
                     /locus_tag="VFH83_08855"
     CDS             1948..2391
                     /gene="secE"
                     /locus_tag="VFH83_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET6486514.1"
                     /translation="MASNDATTVANRSAMTPERLVVIFTLCLAIVLVLFLKHVIAPVW
                     VAVGLPDRPLIEGAEEWTYSSLVAIGLTAALLGGAWANTRVRHLAFETASELMRVTWP
                     SWEETRVSTVAVIVASLVAGVILFFIDTLSYKLMVEWLPALWGKL"
     gene            2391..>2718
                     /locus_tag="VFH83_08860"
     CDS             2391..>2718
                     /locus_tag="VFH83_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HET6486515.1"
                     /translation="MADKRWYAVHTYSGFENKAKKSLEEKIRLEGLQDSFGEILIPVE
                     QVVEMVKGEKKTSKRKFLPGYILVQMEMNDRTWHLVKNTPKVTGFIGAAANEQPPPIP
                     DREMERL"
BASE COUNT          549 a          817 c          944 g          408 t
ORIGIN      
        1 agcgcggcgg gtgagaagac ggaggacggc agagggatcg gtggttgaag gaggcacgag
       61 tcggaggggt acccaagtgg ccaaagggag cagactgtaa atctgccggc tctacgcctt
      121 cgttggttcg aatccaaccc cctccaacga ggccagacgg ggagaggcac aggcgggaat
      181 agctcagttg gtagagcgtc agccttccaa gctgaatgtc gcgggttcga atcccgtttc
      241 ccgctccaac acgacgcagc ggagcacgag accagcagca agccctgata gctcagttgg
      301 tagagcgcat ccttggtaag gatgaggtct ccagttcaat cctggatcag ggctccattc
      361 gaggatcgat agaggagcac aatgagcaag gaaaagttcg agcgcaccaa gccccacgtg
      421 aacgtgggga ccatcgggca cgttgaccac gggaagacca ccctcacggc ggccatcacc
      481 aaggtgctgg ccaagcaggg cggtgccagc tacctggcct acgaccagat cgacaaggcc
      541 cccgaggagc gggagcgcgg gatcaccatc tccacggcgc acgtggagta ccagaccaag
      601 aaccggcact acgcccacgt ggactgcccg ggtcacgccg actacgtgaa gaacatgatc
      661 accggcgccg cccagatgga cggggcgatc ctggtggtga gcgcggccga cgggccgatg
      721 ccccagaccc gcgagcacat cctgctcgcc cgtcaggtcg gcgttccgta catcgtggtc
      781 tacctgaaca aggtggacat gctggacgac cccgagctcc gcgagctggt ggagatggag
      841 gtccgggatc tgctgaagaa gtacgagttc ccgggcgaca agaccccgat catccccggc
      901 agtgcgctga aggcgctgga gggcgacacc agcgagatcg gcgagggctc catcatgaag
      961 ctgatggacg cgctcgacac ctacatcccc accccgcagc gacagacgga caagccgttc
     1021 ctgatgccgg tggaggacgt tttctccatc gccggtcgtg gcaccgtggc caccggccgt
     1081 gtggagcgcg gcaagatcaa ggtcggtgag gaagtggaga tcgtcggcct ccgcccgacc
     1141 acgaagacgg tggtgaccgg cgtcgagatg ttccggaagc tgctcgacga gggcattgcc
     1201 ggggacaaca tcggggcgct gctccgtggc ctcaagcgcg aggacgtgga gcgcgggcag
     1261 gtgctggcca agccgggcag catcacgccg cacaccaagt tcaaggcgca gatctacgtc
     1321 ctgaccaagg aggagggcgg gcgccacacc ccgttcttca agggctaccg gccgcagttc
     1381 tacttccgca ccaccgacgt gaccgggacg gtgaagctcc cggacaacgt ggagatggtg
     1441 atgccggggg acaacatcgc gatcgaggtg gagctgatca cccccatcgc catggagaag
     1501 gagctccggt tcgccatccg cgagggtggc cgcacggtgg gcgcgggagt cgtcgccgag
     1561 gtgctcgagt aagggtagga ttgcccgccc cgggcgccgg ggtggacggc ctcgggaggg
     1621 gagagctgcc gcgcaggtgg ctttcccctc cttgccgttt ggagaggaag agaagtcatg
     1681 ccgaagggaa acaggagcat catctcgctg gagtgtccgg tctgtaagcg ccggaactac
     1741 tcgacgacca agaaccggcg aaagagccag gacaagctcg agctgagcaa gttctgcccc
     1801 tggtgccgga agcacaccga ccacaaggaa gggaaggtct gaggtcagcg aagttctagg
     1861 ccagtagctc caacggtaga gcagcggtct ccaaaaccgc gtgttggggg ttcgaatccc
     1921 tcctggcctg cagtcctcga gaacaccatg gcatccaacg acgccaccac cgtcgccaat
     1981 cgctccgcga tgacgcccga gcggctggtg gtcatcttca ccctctgcct cgccatcgtg
     2041 ctggtgctgt tcctgaagca cgtcatcgcc ccggtctggg tcgcggtggg gctcccggac
     2101 cggccgctga tcgagggcgc ggaggagtgg acctacagct cgctcgtcgc gatcgggctg
     2161 accgccgcgc tcctgggcgg ggcctgggcg aacacccgcg tccggcacct ggccttcgag
     2221 accgcctcgg agctgatgcg ggtgacctgg ccgagctggg aggagactcg ggtctcgacg
     2281 gtcgcggtga tcgtggcctc gctggtcgcg ggggtcatcc tcttcttcat cgacaccctg
     2341 agctacaagc tgatggtcga gtggctgcct gcgctgtggg ggaagctgtg atggctgaca
     2401 agcgctggta cgcggtccac acctactcgg ggttcgagaa caaggcgaag aagagcctgg
     2461 aggagaagat ccgcctcgag gggctccagg actcgttcgg cgagatcctg atcccggtgg
     2521 agcaggtggt ggagatggtg aagggggaga agaagacctc caagcggaag ttcctccccg
     2581 gctacatcct cgtgcagatg gagatgaacg accggacctg gcacctggtg aagaacacgc
     2641 cgaaggtgac ggggttcatc ggggcggcgg ccaacgagca gccgccgccg atcccggacc
     2701 gggagatgga gcgtctga
//
LOCUS       DASRZS010000366         3673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2894886, whole genome shotgun sequence.
ACCESSION   DASRZS010000366 DASRZS010000000
VERSION     DASRZS010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2894886"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VFH83_08865"
     CDS             complement(<1..419)
                     /locus_tag="VFH83_08865"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HET6486516.1"
                     /translation="MTQFRNRVLFLGCGAVAECTLPIFFRLIEVDPHRVTILDFEDRA
                     PLLREWMGRGATVVRDRVTPANLGALLGNHLSRGDLLVDLAWNIDAGDILQWCHDHGV
                     LYVNTSTEVWDPYGAHGHPTTRTLYARQMNLRARISR"
     gene            633..2792
                     /locus_tag="VFH83_08870"
     CDS             633..2792
                     /locus_tag="VFH83_08870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6486517.1"
                     /translation="MKSYSVLPIAMAAVSLTVAFYESLTLPRRKDRPRNIAFTLTCIG
                     VALYDLACGGQYNVDTALQSLPWLKLQVAVLNLAGFCLVWYFALETRKIPRAILVVFG
                     VWFGLGALSQTLNLGDLTWILSHPIDKPRALPFGLSVVYHEVDTGILTDLQNAAGMVT
                     ILYVLTVVIRYARSGHRREARPLFFLIGILCASYVNDYLTDENIITSIYTLEYAWLAA
                     VVLVGLQRARQLIEASAVKQDLAESEEKFRLFIEQSSEAILISDERGRVVECNLAAER
                     LAGQGRERILGTPIWQVGVRAVQGPALGAVQRPAEGERRGQEVTGGQIEDEIERPDGT
                     RRMVRQNLFAIRTGKGYRIGSISYDITEIRRTEEALRESERRYRAIFESLQDVYFRTD
                     AQGVVRLLSPSVRSAFGLDSGEVVGRPITEILREAGGTEAILARIAATGSIADAELRL
                     RGSEGRSFEVSLSAHWLLDGQGRLEGYEGMVRDISDRKRAEEKVLASLEEKSMLLKEI
                     HHRVKNNLQIISSLLFLQESRIGEAVYRGVLQECRNQVLSMALIHEDLYRSADFRSVD
                     FGGYVRNLAGRLMGLYGASRVRLRTEVEEIRLGIDKAIPCGLIVNELCTNALKYAFRG
                     KEGGELRVELHRVKEGRSGGPEVRLRVADDGVGLPAGMSVRSAETLGMQIVGGLVDQL
                     RGRLAVQSPAPGTVPSSAPGGVRSGRGTCIDIEFPES"
     gene            2866..>3673
                     /locus_tag="VFH83_08875"
     CDS             2866..>3673
                     /locus_tag="VFH83_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6486518.1"
                     /translation="MKKARILIVEDEELVALGIRLFLQSIGYEVLPLLSSGEETVERF
                     GELSPDLVLMDIRLEGTMSGIEAAAALRTRYPVPVVFLTAYSDADTMRGARHVSPYGY
                     ILKPFDEQTLAAMIAVALRKSALQQELRERARRGTAGMMGDRSVEGSSGAPPVGGSAP
                     WVPDAGLVEAGPLRMGGFLVPSAFGAGDRYGIFRIGDSQVGMYAVDVLDHGDTAGSQG
                     RSLSELFTIQGARFATLDADPLVPRVVAEKLNDRLCQRDDHAYVSFGYGVF"
BASE COUNT          608 a         1055 c         1371 g          639 t
ORIGIN      
        1 cagcgcgaga tacgggcgcg caggttcatc tgtcgggcgt agagcgtccg cgtcgtcggg
       61 tgcccgtggg cgccgtaagg gtcccacacc tccgtggagg tgttgacgta gagcaccccg
      121 tggtcatgac accactgcag gatgtctccg gcatcgatgt tccacgccag atccaccagc
      181 aggtcgccgc gggagagatg gttccccaga agcgctccca ggttggcggg cgtgacacgg
      241 tcccggacca cggtggctcc tcggcccatc cactcccgca gaaggggagc gcggtcttcg
      301 aagtccagga tcgtcacgcg atggggatcc acctcgatga gccggaaaaa gatgggcagg
      361 gtgcattcgg ccaccgcgcc gcagcccaga aagaggaccc ggtttcggaa ctgtgtcatg
      421 gcgcgctctt gcctcccgtc gaacgctgac cgtggtgctt cgccatcttc gggacgcact
      481 atagggtcat tcgaacggaa ttggcaatat gcgaggtcga cgcggttgac gtggcccacg
      541 ctgcggcaca cggctcctct ccgccacgcg cggctcctct ccgccacgcc ctcccatggg
      601 aaccccggtc cggatgaact atagttgttt ccgtgaagtc gtactcggtc ctgccgatcg
      661 ccatggcggc tgtcagcttg accgtggcct tctacgagag tctgaccctg cctcggcgga
      721 aggaccgccc tcgaaacatc gcgttcacgc tcacctgcat cggcgtggcc ctctacgacc
      781 tggcctgcgg cggccagtac aacgtggaca cggctctgca gagcctcccg tggctgaagc
      841 tccaggtggc ggtgctgaac ctggcagggt tctgcctggt gtggtacttc gccctggaga
      901 ctcggaagat cccccgcgcc atcctggtcg tgtttggcgt ctggttcggg ctgggagccc
      961 tctcccagac gctaaacctc ggagacctca cgtggatcct gtcccatccg atcgacaaac
     1021 cccgggcgct cccgttcggt ctttcggtgg tctatcacga ggtggatacc ggaatcctga
     1081 ccgacctcca gaacgcggcg gggatggtga ccatcctgta cgtattgacc gtggtgatcc
     1141 gatacgcgcg ttcggggcat cgccgcgagg cccgtcccct cttcttcctc attggcatcc
     1201 tctgcgcctc ctacgtgaac gactacctca cggacgagaa catcatcacg tcgatctaca
     1261 cgcttgagta cgcctggctg gccgcggtgg tcctggtggg gctgcagcgg gcccgacagc
     1321 tcatcgaggc ttccgcggtg aaacaggacc ttgcggagag cgaggagaag ttccgcctgt
     1381 tcatcgagca gtccagcgag gcgatcctga tcagcgacga gcgaggacgg gtggtggagt
     1441 gcaaccttgc ggcggagcgg ctggcgggac agggacggga gcggatcctg ggcacgccga
     1501 tctggcaggt aggggtgcgg gcggtgcagg ggcccgccct aggggcggtg cagcggcccg
     1561 ccgaggggga gcggcgaggg caggaggtga cggggggcca gatcgaggat gagatcgaga
     1621 ggcccgacgg gacgcggcgg atggtgaggc agaacctgtt tgccatccgc acgggcaagg
     1681 ggtatcgcat cgggagcatc agctacgaca tcacggagat acggcgcacg gaggaggcgc
     1741 tgcgggagag cgagcggcgg tatcgggcga tcttcgagag cctgcaggac gtgtactttc
     1801 gcaccgacgc gcagggggtg gtgcggctgc tgagcccatc ggtgcgttcg gcctttgggc
     1861 tggacagtgg ggaggtggtg gggcggccga tcacggagat cctgcgggag gccgggggaa
     1921 cggaggcgat tctggcgcgg atcgcggcga cggggtcgat cgcggacgcg gagttgcggc
     1981 tgcgggggtc ggaggggcgg agctttgagg tatcgctgag cgcgcactgg ctgttggacg
     2041 gacaggggag gctggaaggg tacgagggga tggtgaggga catcagcgac cgcaagcgag
     2101 cggaggagaa ggtgctggcg tcgctggaag agaagagcat gctgttgaag gagatccacc
     2161 atcgggtgaa gaacaatctg cagatcatct cgagcctgtt gtttttgcag gagagtcgga
     2221 tcggggaggc ggtgtatcgg ggggtgctgc aggagtgccg caaccaggtg ttgtcgatgg
     2281 cgctgatcca cgaggatctg taccgctcgg cggactttcg cagcgtggac tttggggggt
     2341 acgtgcgcaa cctggcgggg cggctgatgg ggctgtacgg agcctcgagg gtacggctgc
     2401 gaacggaggt ggaggagata cggttgggga tcgacaaggc gatcccctgc gggctgattg
     2461 tcaacgagct gtgcaccaat gcgctgaagt acgcgtttcg ggggaaggag ggaggggagc
     2521 tgcgggtgga gctgcatcgg gtgaaggagg ggcggtcggg cggacctgag gtgcggctga
     2581 gggtagcgga cgacggggtg gggctgccgg cggggatgtc ggtgaggagc gcggagacgt
     2641 tggggatgca gattgtgggg gggctggtgg atcagctgcg ggggaggctg gcggtgcagt
     2701 cgcccgcccc agggacggta ccgtcgtccg ccccaggggg cgtgcggagc ggccgcggta
     2761 cctgcatcga catcgagttc cccgaatcgt gagaggattc gcggtgagga aacgatccgt
     2821 aatttgtgat atattgagcg tggcctatca ccaaacggcg acctcatgaa gaaggcaaga
     2881 atccttattg tagaagacga ggagctcgtt gccctcggga tcagactgtt cctccagagc
     2941 atcggctacg aggtactgcc cctgctttcc tcgggagagg agacggtgga gcgtttcggg
     3001 gagctgtctc cggacctcgt gctgatggac atccgcctgg aagggaccat gtcggggatc
     3061 gaggccgcgg cggcgctgcg gacccggtat ccggtgcccg tggtgtttct gacggcgtac
     3121 tccgacgcgg acaccatgcg cggcgcccgc cacgtcagtc cctacgggta catcctgaag
     3181 cccttcgatg agcagaccct ggccgccatg atcgccgtgg cccttcgcaa gtcggccctc
     3241 cagcaggagc ttcgggagag ggcccgaagg ggcaccgcgg ggatgatggg ggaccgctcc
     3301 gtcgaaggct cgtccggcgc tccgccggtc ggcggctccg cgccctgggt ccccgatgca
     3361 gggctcgtgg aggccggccc gcttcgcatg ggtggattcc tggtcccatc cgcgttcggg
     3421 gcgggggatc gatacgggat ctttcgcatc ggcgactccc aggttgggat gtacgcggtc
     3481 gacgtcctgg accacggtga taccgccgga tcccaagggc gctccctctc ggagcttttc
     3541 acgatccagg gcgcgcgctt cgcgaccctg gacgcggacc cgctggtccc gcgcgtcgtg
     3601 gcggagaagc tgaacgatcg gctgtgccag cgggacgacc acgcctacgt ctccttcggc
     3661 tacggagtct ttg
//
LOCUS       DASRZS010000367         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5592214, whole genome shotgun sequence.
ACCESSION   DASRZS010000367 DASRZS010000000
VERSION     DASRZS010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5592214"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VFH83_08880"
     CDS             <1..795
                     /locus_tag="VFH83_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6486519.1"
                     /translation="ARKLAEDRLRLASVAADAANKAKSDLLAGMSHDLRTPLTAIIGY
                     AEILTLEYFGTVNARQKQQLEVILQCSRHLLELINDILDISRIESGKTELDITSVRAG
                     ALLQGAVELLAENAVQRKIALRCSISDEVRELDIPADERRVRQVLLNLLSNALKFTPE
                     DGSVEISMSAMDGALEVSVQDSGIGIPRQELERIFDAFYQIKGRSSASMESGSGLGLA
                     LARQIVELHGGRIWAESDGIGKGSRLTFTLPLPKGTPKAPVSASLS"
     gene            828..1814
                     /locus_tag="VFH83_08885"
     CDS             828..1814
                     /locus_tag="VFH83_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET6486520.1"
                     /translation="MIRMAYRRSDGSLDPDPRVERIPAALADPSGLLWLDFVEEPADA
                     CERILSDLFHFHPLAIDDALRETHVPKVDDWGTYLYLVLHATKLDPAADELLQSIELD
                     LFVGPNYVVTHRDKSIAPLEALWQSCAKDDRILGRGAARLLYHIVDETVGSHLPVVDD
                     LDEEMDRVEERILSTPEPAVLSQVLRIKRALLHLRRIVAPQRETLNRLARDQLSVIEA
                     GQRVYFRDVYDHLVRLHDLTEGLRDLAAGALDTYLSVVNNRMNEVMKTLTTIATLFMP
                     ISFLTGFFGMNFFSASNPPAPWTGRVMLGVVCCVTVVVPTVMFLWLRRRRWI"
     gene            complement(1913..2614)
                     /gene="deoC"
                     /locus_tag="VFH83_08890"
     CDS             complement(1913..2614)
                     /gene="deoC"
                     /locus_tag="VFH83_08890"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET6486521.1"
                     /translation="MLRAVDIALMIDHSLLRPELTQQDVVEGCRLARELETKSVCVAP
                     CNVKLAAGELAGSPVLTTTVIGFPHGYQATEVKAAEARRALEDGAAELDMVINIGWLR
                     SGAYDRVEADIRGVVELAHARRVIVKVILENCYLTDAQKVEGCRIAERAGADFVKTST
                     GFGSGGSTLEDLRLMRRTVGPSVQVKAAGGVRTLDAALAVRATGATRFGATATRAILE
                     EARRREAAGTLKLPD"
     gene            complement(2598..>3683)
                     /locus_tag="VFH83_08895"
     CDS             complement(2598..>3683)
                     /locus_tag="VFH83_08895"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET6486522.1"
                     /translation="GFGFGVPLVTPTDRHGKVDHAAARRLWEFLIPQLDVAVVLGTTG
                     RGRFIINEEPAEARRLVDLGVELCGRHRTPLVIGTGAETVERAKELTAYAAERGVYAV
                     LVTPPVFAASPFARSLGHNPLSAGYQDRLLREYFFPILDAVPRGSTTRFLPYVFPTLT
                     DADPRAFLRPEILERLWERAAGSGRTLDGGKLTLRDDAVALDYSEKCHELSFQAGMDS
                     TVHAFLGNPRMHFSGAILGSGNLIPKTLKTHVADCLRLREAVRGNSPEAAHLTRVVLS
                     RQNQVSALFNDLWDSGLFGPLIHGFLGIGSPYQVAPAAASELGEWARVIGTDAPTLVD
                     EIASYCPESPLSREARAAAEKTHAARR"
BASE COUNT          601 a         1280 c         1207 g          595 t
ORIGIN      
        1 gcccgcaagc tggcggagga ccggctgcgc ctggcatccg tggccgcgga tgccgccaac
       61 aaggccaagt ccgatctcct ggccggcatg tcgcacgacc ttcgcacgcc gctcaccgcc
      121 atcatcggct acgcggagat cctcaccctg gagtacttcg ggaccgtcaa cgcccggcag
      181 aagcagcagc tcgaggtgat cctccagtgc tcccggcatc tcctggagct catcaacgat
      241 atcctggaca tctcccgcat cgagagcggc aagaccgagc tggacatcac cagcgtccgg
      301 gccggcgctc tgctgcaggg cgccgtcgag ctcctggcgg agaacgccgt gcaacggaag
      361 atcgctctgc gatgcagtat ctccgacgag gtccgggagc tcgacatccc ggcggatgaa
      421 cggcgcgtcc gccaggtgct cctcaacctc ctctccaacg cgctgaagtt cacgccggag
      481 gacggctccg tggagatctc catgagcgct atggacgggg ctctggaggt gagcgtccag
      541 gactccggca tcgggatccc gcggcaggag ctggagcgga tcttcgacgc attctaccag
      601 atcaaggggc gctcctccgc ctcgatggag agcgggtccg gcctggggct ggccttggcc
      661 cgccagatcg ttgagctgca cggcggtcgg atctgggcgg agagcgacgg gatcggcaag
      721 gggagccggc tcaccttcac gctcccgctt cccaagggaa ccccgaaagc ccccgtcagc
      781 gcgtcgttgt cttgaacccg tccggggcct gacctatcct tgccgccatg atacggatgg
      841 cataccggag gagcgacggc tctctcgatc cggacccgcg cgtggaacgg atccccgcgg
      901 ccctggcgga tccttccggc ctcctgtggc tggacttcgt ggaggagccg gcggacgcgt
      961 gcgagcggat cctctcggac ctcttccact tccaccccct cgccatcgac gatgcccttc
     1021 gggaaaccca cgtgcccaag gtggacgact ggggcaccta cctctatctc gtgcttcacg
     1081 ctacgaaact ggacccggcc gcggatgagc tgctccagag catcgagctc gatctcttcg
     1141 tcggtccgaa ctacgtggtg acgcatcgtg acaagtccat cgctcctctg gaagccctgt
     1201 ggcagagctg cgccaaggac gatcggatcc tgggccgcgg ggcagcccgg ctcctctacc
     1261 acatcgtgga tgaaaccgtg ggcagccacc tgcccgtggt ggacgacctc gacgaggaga
     1321 tggaccgggt ggaggagcgt atcctctcca cccccgagcc cgcggtgctg tcccaggtgc
     1381 ttcgcatcaa gcgcgccctg ctccacctgc gaaggatcgt ggcgccccag cgagagaccc
     1441 tgaaccgtct ggcccgggac cagctatccg tgatcgaggc cggccagcga gtatactttc
     1501 gggacgtata cgatcacctg gtccgtcttc acgacttgac cgaggggctt cgggacctgg
     1561 ccgcgggagc gctggacacc tacctctcgg tggtcaacaa ccgcatgaac gaggtgatga
     1621 agaccctcac gacgatcgcg acgttgttca tgcccatctc gttccttacc gggtttttcg
     1681 ggatgaactt cttcagcgcc tccaacccgc ccgcgccctg gacggggcgc gtcatgctgg
     1741 gcgtcgtctg ctgcgtgacg gtggtggttc cgaccgtgat gttcctgtgg ctgcgccgca
     1801 ggcgctggat ctaggccagg gacggccgaa gaagccgtga ggacagggtt gcgtagcgcg
     1861 ccgaagacgc gcggagggcg gcagccgcgc agcgagagcc tccgaccggc tcctagtccg
     1921 gaagcttgag cgtgcccgcg gcctcccgcc tgcgggcctc ctccaggatg gcacgggtgg
     1981 ccgttgcccc gaaccgcgtc gccccagtgg cgcgcaccgc cagcgcggcg tccaacgtgc
     2041 gcacaccgcc agcggccttg acctgcacgc ttgggccgac ggtccgtcgc atcagccgca
     2101 ggtcctccag ggtggagccg ccggagccga atccggtgga ggttttgacg aagtccgcac
     2161 cggccctctc ggcgatccga cacccctcca ctttctgggc gtccgtgagg tagcagttct
     2221 ccaggatgac cttcacgatc acccgacggg catgggcaag ctcgaccacg ccgcggatgt
     2281 ccgcctccac gcggtcgtac gcaccggagc gcagccagcc gatgttgatc accatgtcca
     2341 gctccgcggc gccgtcttcc agcgcccgtc gggcctccgc cgccttgacc tcggttgcct
     2401 gatacccgtg cgggaacccg atcacggtgg tggtcaggac cggacttccg gccagctctc
     2461 cggccgccag cttcacgttg cagggggcga cgcagacgct cttcgtctcg agctcgcgcg
     2521 ccagccggca tccctccacc acgtcctgct gggtgagctc gggcctcaac agggaatgat
     2581 cgatcatcag ggcgatatca acggcgcgca gcatgggtct tctccgccgc tgcccgcgct
     2641 tctcgggaaa gcgggctctc gggacagtac gaggcgatct catcgaccag cgtcggcgcg
     2701 tccgtgccaa tgacccgggc ccattcgccg agctccgagg ccgccgcagg ggccacctgg
     2761 tagggcgagc cgatccccag gaagccgtgg atcagaggac cgaagaggcc gctgtcccac
     2821 aggtcgttga acaaggcgct cacctggttc tgacggctca gaacgacgcg tgtcaggtgc
     2881 gccgcctcgg gggaattgcc acgaaccgct tcgcgcaggc gcaggcagtc cgcgacgtgg
     2941 gtcttcagcg tctttgggat caggttcccg ctccccagga ttgcgccgct gaaatgcatg
     3001 cggggattcc ccaggaacgc atggaccgtc gagtccatac cggcctgaaa cgaaagctcg
     3061 tggcacttct cgctgtagtc cagcgccacg gcgtcatccc gcagggtcag cttgccgccg
     3121 tccagggttc ggccggagcc cgccgcgcgc tcccacagcc gctcaaggat ctcgggccgc
     3181 agaaaggctc gggggtccgc gtccgtgagc gtggggaaca cgtagggcag gaaccgagtc
     3241 gtgctgccgc gggggacggc gtcgaggatg gggaagaaat actcccgcag gagtcggtcc
     3301 tggtagcccg ccgagagcgg gttgtggcca agggacctgg caaacggcga ggcggcgaac
     3361 acgggcggcg tcaccagcac ggcgtagacg ccgcgctcgg cggcgtaggc agtgagctct
     3421 ttggcccgct ccacggtctc ggcgccggtt ccgatcacca gcggcgtgcg gtgcctccca
     3481 cacagctcca cgcccagatc cacgaggcgc cgggcctctg ccggctcctc gttgatgatg
     3541 aaccggcccc tgccggtcgt tcccagcacc actgccacgt cgagctgggg gatcaggaac
     3601 tcccagagtc ttcgagcggc cgcgtgatcc accttgccgt gcctgtccgt cggagtcacc
     3661 aggggaacgc caaagccaaa gcc
//
LOCUS       DASRZS010000368         4134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2125248, whole genome shotgun sequence.
ACCESSION   DASRZS010000368 DASRZS010000000
VERSION     DASRZS010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2125248"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2435)
                     /locus_tag="VFH83_08900"
     CDS             complement(<1..2435)
                     /locus_tag="VFH83_08900"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET6486523.1"
                     /translation="MIGNGSPLSVINLSWLEELHREWRADPGSVDGAWDTLFRDTESN
                     RLLEDSVNGPTSLVSSDQVYKQSRVDSLIWSYRDVGYLYAHLNPLAPAEGEQRNYYAE
                     PEHTYEQLTLAEYDLSEQDLDSEYSAGRAFLPSRMKLSELIKALQQTYCSTVAVEFLH
                     IQNKPIRRWLIQQMESTRNRPSFDRTKKRIVLEDLISAEEFEHFMHSAFIGQKRFSLE
                     GAEVVIPALHQIVDSAPGMGIQEIILGMTHRGRLTVLNRIMGKPLEEVFAEFEGLEDP
                     GDYGGGDVRYHLGYQRDHVTPDGSVHLNLVANPSHLESVDPVVQGMARAVQREQGDSE
                     RRKVVPIVLHGDAAFSGQGIVSETFNISTLKGYKTGGTIHIIINNQIGFTTSMRDARS
                     TFFPTDVAKMLPVPVFHVNGDDPEAVVYTVDLALRFRQTFGRDVVVDIFCYRKYGHNE
                     GDDPSFTHPLMYRIIEKKKSQPTIYTEKCLQEGIVTQEEEQAFRADLRASLRAALQEA
                     RSAGGEVVEKSPAQGPNAYRWGDVGVIPSEDRLREVIRVLTTQPPGFHMHPKLKRIVT
                     DKLARLSKDGAVDWAFAESLAFGTLLQEGIPVRLSGQDSARGTFSQRHLVWWDSETLQ
                     PLPYTPLNALATERSRFSVYDSPLSEYSILGFEYGHSLGQPRALVMWEAQFGDFSNGA
                     QVIIDNYIAAGEAKWGSVSGLVMLLPHGFEGQGPEHSSAHLERFLLLCARENIQVCNC
                     TTPAQYFHLLRRQATRGFKKPLVVMTPKSLLRHPQAVSPLRAMHADEFHEVILDPHPP
                     ARTRRLLLCSGKV"
     gene            complement(2432..3319)
                     /gene="sucD"
                     /locus_tag="VFH83_08905"
     CDS             complement(2432..3319)
                     /gene="sucD"
                     /locus_tag="VFH83_08905"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET6486524.1"
                     /translation="MSILLGKDSRVIVQGITGRDGSFHARSMREYGTSVVGGVTPGKG
                     GQTLEGIPVFDTVKEAVRETRANATVIFVPAKAASAALLEAAREHVPLIVCITEGLPI
                     QEMLSNVRQLDGSRLIGPNCPGLITPGQAKAGIMPGRIHKPGDIGVISRSGTLTYEVV
                     WALTRAGMGQSTCIGVGGDPIIGTGFVELLSLFEADPGTAGVVLIGEIGGEDEERAAD
                     YIRLSMKKPVVSFISGRSAPPEKRMGHAGAIVSGGRGTAEAKVGALRAAGVPVADRPD
                     EIPGLMATRLGRSTQEGRS"
     gene            complement(3316..>4134)
                     /locus_tag="VFH83_08910"
     CDS             complement(3316..>4134)
                     /locus_tag="VFH83_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET6486525.1"
                     /translation="GMEIEEIAKTQPDRIVTFSVPASRGLDRKAHFQELSRAFAPSAV
                     AEQAWKILGEIYRLFTEKDCLLVEINPCAQTPAGTLVAADAKVIFDDNALYKHPELES
                     LRSPEEYSDEEIDARSNGLSYVGLDGTIGCIVNGAGLSMATMDLIKFFGGSPANFLDV
                     GGSSSPEKVLHALRIVLKHPGVSAVLINIFGGITRCDDIARGILMAREKLSIDVPLVI
                     RLIGTNEAEGRAILKGAGLDAFEDLTPAVQAVISRSREAVRTGGGVEPRPRGAA"
BASE COUNT          659 a         1393 c         1351 g          731 t
ORIGIN      
        1 tagaccttgc cgctgcaaag gaggagccgc cgcgtgcgcg ccggcggatg gggatccagg
       61 atcacctcat ggaactcgtc cgcgtgcatg gcccgcaggg gagagaccgc ctgcgggtga
      121 cggagcaggc tcttgggcgt catgaccacc aggggcttct tgaagcctcg ggtggcctgc
      181 cgcctcagga gatggaagta ctgcgccggc gtggtgcagt tgcagacctg gatgttctcg
      241 cgggcgcaca agaggagaaa ccgctccagg tgcgcgctgg aatgctcggg gccctgaccc
      301 tcgaacccgt gcggcaagag catcaccagg ccgctgaccg acccccactt ggcctcgccg
      361 gcggcgatgt agttgtcgat gatgacctgg gcgccgttgg agaaatcccc gaactgcgcc
      421 tcccacatca ccagggcgcg cggctggccc agggagtgtc cgtattcgaa gcccaggatc
      481 gagtactccg acagcgggct gtcgtagacg gagaaccgcg atctctccgt tgccagcgcg
      541 ttcaacgggg tgtacggaag gggctggagc gtctcgctgt cccaccacac caggtgacgt
      601 tgggagaacg tcccgcgggc gctgtcctgt ccgctgagcc gcaccggaat cccctcttgc
      661 agaagggtcc cgaaggccag ggactcggcg aacgcccagt cgacggcgcc gtccttcgat
      721 agccgcgcca gcttgtccgt cacgatcctc ttgagcttgg gatgcatgtg gaatccgggc
      781 ggctgggtgg taagcacccg gatcacctcc cgcaggcggt cctccgacgg gatcacgccc
      841 acgtcgcccc accgatacgc gttgggcccc tgggcggggg acttctcgac cacttcccct
      901 cccgcggacc gcgcttcctg cagggccgcg cgaagggagg cgcggagatc cgctcggaag
      961 gcctgctcct cctcctgggt gacgatgccc tcctgcaggc acttctccgt atagatcgtg
     1021 ggttggctct tcttcttctc gatgatgcgg tacatcagcg gatgggtgaa cgaggggtcg
     1081 tccccctcgt tgtggccgta cttgcggtag cagaagatgt ccaccacgac gtcccgcccg
     1141 aaggtctggc ggaagcgcaa ggcaaggtcc accgtgtaga ccactgcctc cgggtcatcg
     1201 ccgttcacgt ggaagacggg caccggcagc atcttggcga cgtccgtggg gaagaacgtg
     1261 gagcgcgcgt ccctcatgga ggtcgtgaag ccgatctggt tgttgatgat gatgtggatc
     1321 gtgccgcccg tcttgtagcc cttgagcgtg gagatgttga aggtctccga cacgatgccc
     1381 tggccggaaa acgcggcgtc cccgtgcagg acgatgggca ccaccttgcg ccgctcggag
     1441 tccccctgtt cccgttgcac cgcgcgcgcc atcccctgga ccaccggatc cacggattcc
     1501 aggtggctgg gattggccac caggttcagg tggaccgaac cgtccggcgt gacatggtcc
     1561 cgttggtagc ccaggtggta gcggacgtcc ccgccgccgt aatcccccgg gtcctccagg
     1621 ccctcgaact cggcaaagac ctcctccagg ggcttgccca tgatccggtt caggaccgtg
     1681 agccggccgc gatgggtcat tcccaggatg atctcctgga tccccatgcc gggcgcgctg
     1741 tccacgatct ggtgcagagc ggggatcacc acctcggcgc cttccaggga gaagcgcttc
     1801 tgcccgatga aagcgctgtg catgaagtgc tcgaactcct ccgcggagat caggtcctcc
     1861 agcacgatgc gcttcttcgt ccggtcgaac gaggggcggt tgcgagtgga ctccatctgc
     1921 tggatgagcc accggcggat cggcttgttc tggatgtgca ggaactccac ggccaccgtg
     1981 gagcagtagg tctgctggag cgccttgatc agctcggaga gcttcatccg ggacggaagg
     2041 aaggcgcgcc ccgcggagta ctcggaatcc aggtcctgct cggagagatc gtattccgcc
     2101 agggtgagct gctcgtaggt gtgctcgggc tcggcgtagt agttgcgttg ctccccctcc
     2161 gcgggggcaa gaggattcag gtgcgcgtag agataaccga cgtcgcggta gctccagatg
     2221 aggctgtcca cccggctctg cttgtacacc tggtccgacg agaccaggga ggtgggtccg
     2281 ttgacgctgt cctcgagcag acggttgctc tccgtgtccc gaaacagcgt gtcccacgcg
     2341 ccgtccaccg accccgggtc ggctcgccat tcccggtgca gctcctccag ccacgagagg
     2401 ttgatcaccg acaacgggga accgtttccg atcatgagcg accctcctgc gtgctgcgcc
     2461 ccaatcgcgt cgccatgagc ccggggatct cgtccggtcg gtcagcaacg gggaccccgg
     2521 ccgcccgcag ggcgccgacc tttgcctccg cggtgcccct gccaccggag acgatggccc
     2581 cagcgtgtcc catgcgcttc tccggcggcg ccgatctccc ggaaatgaac gagacgacgg
     2641 gcttcttcat ggatagccga atgtagtccg ccgcgcgctc ctcgtcctca ccgccgatct
     2701 caccgatcaa caccaccccg gcggtgcccg ggtccgcctc gaagagggag agaagctcca
     2761 cgaacccggt gccgatgatc ggatcccctc ccaccccgat gcaggtggat tgccccatgc
     2821 cggcgcgcgt gagcgcccag accacctcat aggtgagcgt gccgcttcgg gagatgaccc
     2881 cgatgtcccc cggcttgtgg atgcggcccg gcatgatgcc cgccttggcc tgccccggcg
     2941 tgatgaggcc cggacagttc gggccgatga ggcgggaacc gtcaagctgc cggacgttgc
     3001 tcagcatctc ctggatgggc agaccctccg tgatgcacac gatgaggggg acgtgctccc
     3061 gcgcggcctc cagcagggct gcgctggcgg ccttggccgg cacgaagatg accgtggcgt
     3121 tggcccgcgt ctctctcacg gcctccttga ccgtgtcgaa cactggaatg ccctccaggg
     3181 tctgtccgcc cttgcccggc gtcacgccgc ctaccacgct tgtcccgtac tctcgcatgg
     3241 accgggcatg gaacgagcca tcccgccctg tgattccctg cacaatgact cgcgagtctt
     3301 tgcccagcag gatgctcatg ccgcccccct tgggcggggc tctacgccgc ccccggtccg
     3361 aaccgcttct cgggagcggg agatgaccgc ctggactgcc ggcgtcagat cctcgaaggc
     3421 atccagcccc gcgcccttca ggatggcgcg gccctccgcc tcgttggtcc cgatcagccg
     3481 gatcacgagg ggaacgtcga tggagagctt ctcccgcgcc atcaggatgc cccgggcaat
     3541 gtcgtcacac cgcgtgatgc cgccgaagat attgatcagc acggcgctca ccccgggatg
     3601 cttcagcacg atgcgaagcg cgtgcaggac cttctccgga ctggagcttc ctcccacgtc
     3661 caggaagttg gccgggctgc cgccgaagaa cttgatgagg tccatggtgg ccatggagag
     3721 gccggcgccg ttgacgatgc agccgatggt gccgtccagg cccacgtagg agagcccgtt
     3781 ggaccgggca tcaatctcct catcgctgta ttcctcaggg cttcgcaggc tctccagctc
     3841 cgggtgcttg tacagggcgt tgtcgtcgaa gatcaccttg gcatccgccg ccaccagcgt
     3901 cccggcgggc gtctgcgcgc atgggttgat ctccacgagc aggcagtcct tttccgtgaa
     3961 gagccggtag atctctccga ggatcttcca ggcctgctcc gcgaccgccg acggggcgaa
     4021 ggcgcgcgag agctcctgga agtgtgcctt gcggtccaga ccgcgggacg ccggcacgga
     4081 gaaggtcacg atgcggtccg gctgggtctt cgcgatctcc tcgatctcca tgcc
//
LOCUS       DASRZS010000369         8607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2516122, whole genome shotgun sequence.
ACCESSION   DASRZS010000369 DASRZS010000000
VERSION     DASRZS010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8607
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2516122"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="VFH83_08915"
     CDS             complement(<1..1371)
                     /locus_tag="VFH83_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6486526.1"
                     /translation="MSRSFLLFQRVSFAYDSSLTLLFQNLSLSFPSGWTGIVGANGTG
                     KTTLLRLAAGELSPDDGSIRRPGAVVLCPQRTDFPPECLRGLLSATDPLGARLRGQLD
                     LAPDWGDRWESLSHGERKRAQIGAALWQDAAVLCLDEPTNHVDTAARRMLGVAMSQFE
                     GIGILVSHDRHLLDGLCRQCLFLDPPRAVLRPGTWSQGREQARQEDESAMRAAHAARA
                     EVKRVERRLVEAREEARRSHQRLSKRNLKRHDSDARAKLTLAKLTGKDASAGGLVHRL
                     ESNLERAQGRAATSTPRPAYELGLWFSSERARRDTVLSCPADQIPLGGGASLRHPDLV
                     LRPQDRVALVGPNGSGKSTIVRHLVERLSLPESRMPRDRLVYLAQEISLPESAEVMAR
                     LRALSDAELGRALTVVNLLGTDPKRILATGQPSPGEVRKTLIAMGIARIPHLILLDEP
                     TNHLDLP"
     gene            complement(1493..2779)
                     /locus_tag="VFH83_08920"
     CDS             complement(1493..2779)
                     /locus_tag="VFH83_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6486527.1"
                     /translation="MREKRRWFWILVLVLPAAAVVFLAGMAADRWVFQVSDPPSAVSS
                     PPDFDLIRQAWNLIDEKFVDRTALKTQPLTYGAISGMVDALGDTGHSSFLSPDAVKDE
                     QSFMRGQYVGVGLEIQNKNDQVMIVAPLDGSPAIKAGLRAGETIQKVDGTTVENLTLN
                     EVVQRIIGPVGTSVTLTIYDPVARTIFDVTLQRAQIRVSNVSWAPLPGTALADLRVAA
                     FSSGVARQVREALAEIQQQRMAGVVLDLRNDPGGELQEAIGVASQFLASGNVLMEKDV
                     QGAVHADPVRPGGIAPSMPMIVLIDRGTASGAEIVSGALQDAKRGTLIGETTFGTGTV
                     LSAFPLSDGSQLLLATTEWLTPQGRTIWHNGVTPDVSLVMPPDGEILTPVSIKTMSAD
                     EIRGSKDNQLQKAMQMLQEQIQAPPTKAAMARTGSR"
     gene            complement(2919..3887)
                     /locus_tag="VFH83_08925"
     CDS             complement(2919..3887)
                     /locus_tag="VFH83_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET6486528.1"
                     /translation="MPVASEGLWYLGERSLEVRPVKVPDPGPDEVLVQIEACGICTWD
                     IVSYLGKFARYQRYPFCAGHEGVGRVIKAGPRVTIAREGQRVVLHEVPVGTPGGALMA
                     RHAVRPQRQVAVVPEGPIPIHHWIVEPAACIVNGILYAGIEPGDRVALVGAGYMGLLF
                     VQALRKTLASRVCAFDVQPDRLALAARLGASQTWNLKERPVAAEVARSFDVVIETAGT
                     QDSLKTAVALLRPGGILENFAWHHHEASFDLEDWHVNGWRILNIQPGMNPHFGDLYPR
                     TISLIEAGILSNAELVTHWGPVSKAKEIFQSAADKSNGYIKGVITF"
     gene            3964..5241
                     /locus_tag="VFH83_08930"
     CDS             3964..5241
                     /locus_tag="VFH83_08930"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009592118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET6486529.1"
                     /translation="MPVIAVVGANWGDEGKGKITDYLAREADFVVRYQGGNNAGHTII
                     NEYGKFALHLLPSGVFYPHVVNVLGPGVAVNLAAFLTEVDKLVERGVPRPAVRVSERA
                     QALLPYHIQFDALEEERLGKESFGSTKSGIAPFYADKYLKIGLQLNELFDEELSRNRL
                     AKALAQKNVLLEKLYGKSPVSIDEMMSQIAGLRDRVAPFLSDTTTLLLDAVESGKTIL
                     VEGQLGTLRDPDHGIYPYPTSSSPLAGFATVGAGVPPWSISRVVAVTKAYSSCVGAGP
                     FVTELHGEEAETLRKRGGDAGEYGATTGRPRRVGWFDCVATAYGCRVQGATEAAITNL
                     DVLGYRDEILVCTAYDLDGTTSGAFPVSRLLARSKPVYASLPGWKCDISSARKFEDLP
                     ERARAYVRFIEERIRTPIRWISIGPRREQTIVR"
     gene            complement(5256..5843)
                     /locus_tag="VFH83_08935"
     CDS             complement(5256..5843)
                     /locus_tag="VFH83_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486530.1"
                     /translation="MDLQGRLWCKDCLVEVLTRSGAGQVHPGWRKLAAGLLSIFPGAG
                     HMFLGLIGKGFALMGLLILSIFLVLLYSDATGMYWLTAYLVPSLGVLFLSYAVFDALS
                     IADQAKGKRGPTEPLVEDPAMKAIWERALVNRKTIGWVVLVAGLIGVLRIFSEPFSRW
                     TLERFSVSVSILGLVIPVVLLVLGVMLLRKGRSVR"
     gene            complement(5921..6232)
                     /locus_tag="VFH83_08940"
     CDS             complement(5921..6232)
                     /locus_tag="VFH83_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486531.1"
                     /translation="MEAVYRDALRRPATHSPASRDRPLRALAFRRLGFSLVLTALLLA
                     LSLLIPRVAYPSLAGPARTVMTEPSGGIVKSTFEGAQRAVQGILREPVDTGSQNGGRL
                     R"
     gene            complement(6427..7008)
                     /locus_tag="VFH83_08945"
     CDS             complement(6427..7008)
                     /locus_tag="VFH83_08945"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6486532.1"
                     /translation="MTDGEIIERVQGGQTRHFAELVRRYQDPVYAMALRFLGSPGDAE
                     DTAQEVFLRAFRGLSSFKGEARFSTWLYRITFNLCTDSLRRRKRLDGVTLDRADLVAD
                     DRVDVEDGLLSAEARSRVRQCLDTLDERYRTVVVMLYYQKLSYEQISQVLGVPLKTVE
                     TRLYRARKLLRDSLERAERQPAAPGEIPTGGRR"
     gene            complement(7174..7404)
                     /locus_tag="VFH83_08950"
     CDS             complement(7174..7404)
                     /locus_tag="VFH83_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6486533.1"
                     /translation="MPIFEYECKECKSQFELLVRSDTRVACPSCDSEKLVKRLSLFSA
                     QMKRPADAIPACHTGECGCDLGKCGSGLCGVE"
     gene            complement(7408..8157)
                     /gene="jag"
                     /locus_tag="VFH83_08955"
     CDS             complement(7408..8157)
                     /gene="jag"
                     /locus_tag="VFH83_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HET6486534.1"
                     /translation="MIVKEFEGKNEKEAIDRAIEELGLDRNEIDFEIVEAKRPGILFR
                     GGKVKIRVHLAEDQEEANLQIQDQEMEERIVRGAPVSTRPRSENLPPDGEFEHKVLDF
                     THGLLERMGFPAEVAVNFREDGRLGVNIESDGSGILIGKQGKTLEALQFIVNLAVGRM
                     GGDSTRIVLDTQDYRSRRERSLIRMANHVAEQVRKSRSSRLLEPLNPFERRLIHTSLA
                     RYGDIETVSEGDGLYKRIRVIWRDGREQRRE"
     gene            complement(8177..>8607)
                     /locus_tag="VFH83_08960"
     CDS             complement(8177..>8607)
                     /locus_tag="VFH83_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HET6486535.1"
                     /translation="LYNLLNTHFELRGALFIPGWIPDLSVPEAIVNFPFTIPLLGWTA
                     LRALPLVMVATQLLQGKFTQPTDPSQTGAQMKLLTYALPIVFLFILYDMPSGLVLYWT
                     VQNVLSIFQQLYINALRKKKDALTVVPARPGGKQSQKSGR"
BASE COUNT         1516 a         2817 c         2740 g         1534 t
ORIGIN      
        1 cggcaggtcg aggtggttcg tgggctcgtc cagcaggatc agatgcggga tccgcgcgat
       61 gcccatggcg atcagcgtct tgcgcacctc tcctgggctc ggctgtcccg tcgcaaggat
      121 tcgcttgggg tccgttccca ggaggttgac cacggtcagc gctctgccga gttcggcatc
      181 ggacaatgcc ctcaggcgtg ccatcacctc ggcgctttcc gggagactga tctcctgggc
      241 cagatacacg agccggtctc tcggcattcg gctctccggc aacgacaatc gctcgacgag
      301 gtgccgtacg atcgtgctct ttccggagcc gttggggccc accagcgcca cgcgatcctg
      361 gggccgcagc accagatccg gatgcctcag agacgccccg ccgcccaacg ggatctgatc
      421 cgccggacag gagaggacgg tgtctctccg agcgcgttcg gacgagaacc acagcccgag
      481 ctcgtacgcg ggccgaggcg tcgaggtggc cgcccgtcct tgggcgcgtt ccaggttcga
      541 ctccagccgg tggacgagcc cgcccgccga cgcatccttg ccggtgagct tcgcgagggt
      601 gagcttggcg cgagcgtcgc tgtcgtgacg cttcaggtta cgtttggata gccgctggtg
      661 gctgcgtctc gcctcttcgc gcgcctccac caggcgtcgt tccacgcgct tgacttcggc
      721 ccgagcggca tgcgcggccc gcattgcgct ttcatcttcc tgacgggcct gctcccggcc
      781 ctgcgaccag gtgccgggcc tgagcacggc ccgaggagga tccagaaaca ggcactgccg
      841 gcacagaccg tccagcagat gccgatcgtg gctcaccagt atgccgatcc cttcgaactg
      901 gctcatggcg accccgagca tgcggcgggc cgcggtgtcg acgtggttgg tgggctcatc
      961 gagacagagc acggcggcat cctgccagag agcggcgccg atctgagccc tcttgcgttc
     1021 gccgtgactc aggctttccc atcgatctcc ccagtccggc gccaggtcaa gctgtccacg
     1081 aagtcgagcg cccaggggat cggtcgcgga aaggagtccc cgcaggcact ccggcgggaa
     1141 gtcggtccgc tgcgggcaga gcacgaccgc tccggggcgc cggatggatc cgtcgtcagg
     1201 agacagctct cccgcggcca gccgcaggag cgtggtcttg cccgttccat tcgcgccgac
     1261 gatgcccgtc cagccggacg gaaagctgag ggagagattc tgaaagagga gcgttagtga
     1321 ggagtcgtag gcaaaagaaa cacgttgaaa cagaaggaat gaccgtgaca cggagcgcct
     1381 cctgcggtca tctgccaggt tcggcatctc gcccgatgcc ttggtgcgct cctagtacct
     1441 cacgcccgaa ccctcctgtt gtctgccatc ctacccaggc ccgcggcgct ggtcaacggc
     1501 tccccgtccg ggccattgcg gcctttgtcg gcggcgcctg gatctgctct tgcagcatct
     1561 gcatggcctt ctgaagctgg ttgtccttgc tgccgcgaat ctcgtcggcg ctcatcgtct
     1621 tgatggagac gggagtcagg atctccccgt ccggcggcat gaccaacgaa acatccgggg
     1681 tcactccgtt gtgccagatg gtccgtccct gcggcgtcag ccattccgta gtggcaagca
     1741 ggagctgcga gccatccgag agggggaagg cgctcaagac cgtaccggtg ccaaacgtgg
     1801 tctccccgat caacgtcccc cgtttcgcgt cctggagggc gccactcacg atctccgcgc
     1861 cgctggccgt gccccggtcg atgagcacga tcatgggcat gctgggcgcg attcctcccg
     1921 gacggacggg atccgcatgg acggcaccct ggacgtcttt ctccataagc acgttgccgg
     1981 aggccaggaa ctggctggcc acccctatcg cctcctgaag ctcaccgccc gggtcgtttc
     2041 gaaggtccag gacgaccccg gccatcctct gttgctggat ctcggccagc gcctctctca
     2101 cctggcgcgc aaccccgctg ctgaacgcag ccacccgaag gtccgccagc gcggtgccgg
     2161 gcaacggagc ccagctcacg ttgctgacgc ggatctgggc gcgctgcagc gtgacgtcga
     2221 agatcgtccg ggcgaccggg tcgtagatcg tgagggtcac cgacgttccg acgggaccga
     2281 tgatccgctg caccacctcg ttgagcgtca ggttctccac ggtcgttccg tcgaccttct
     2341 ggatcgtctc tcctgcgcgg agaccggcct tgatcgccgg cgagccgtcc agcggcgcga
     2401 cgatcatcac ctggtcgttc ttgttctgga tctccaggcc gacgcccacg tactgtccgc
     2461 gcatgaagga ctgctcgtcc ttcacggcgt ctggagacaa gaacgagctg tgacccgtgt
     2521 ctcctagcgc gtccaccatg cccgagatgg cgccataggt aagcggctgg gttttcagag
     2581 ccgttcggtc cacgaacttc tcatcgatca gattccatgc ctgccgaatg aggtcgaagt
     2641 ccggaggaga ggacacagcc gacggggggt ccgaaacctg gaagacccac cgatccgcgg
     2701 ccattccggc aaggaacacc acggccgccg cggggagaac gaggacgagg atccagaacc
     2761 accgcctttt ttctctcatg ggctgacatc tcctcgaggg agccgcttgg accgctgcgg
     2821 cgagagtacg atagaagata gcgcaccacc gggactgagg caaagagccg gtccggggca
     2881 tcgcgtcggg aacgtcaggc tgatcaggta cgatcggatc agaaggtgat cactcccttg
     2941 atgtagccgt tgctcttgtc cgcagcggac tggaatatct cctttgcctt ggagactggt
     3001 ccccagtgcg tgaccagctc ggcgttggag aggatccccg cttctataag ggaaatcgtg
     3061 cggggataca agtcaccgaa gtgaggattc atcccgggct ggatgttcag gatccgccac
     3121 ccgttcacgt gccagtcctc cagatcgaag gacgcttcgt gatggtgcca ggcgaagttt
     3181 tccaggatac ccccgggccg aagcaacgcg acggcggtct tcagggaatc ctgggtgccg
     3241 gccgtctcga tgaccacgtc gaacgagcgg gccacttccg cggctaccgg cctctctttg
     3301 agattccagg tctggctggc gccgagacgc gccgcgagcg ccaggcggtc cggctgcaca
     3361 tcgaacgcgc acacccggct ggccagggtc ttccggaggg cctgcacgaa caggagcccc
     3421 atgtaaccgg ctcccaccag cgccactcga tcacccggct cgatgcccgc gtagaggata
     3481 ccgttgacga tgcaggccgc tggctcaacg atccaatgat ggatcgggat ggggccttcg
     3541 gggacgaccg cgacctgtcg ctgaggccgg accgcatgac gggccatcag cgctccgccg
     3601 ggcgttccga ccggaacctc gtgcagcacg acacgctggc cttcccttgc gatcgttacc
     3661 ctgggtcctg ccttgatcac ccgtccgacg ccttcatgtc cggcgcagaa cgggtatcgt
     3721 tggtaccgcg cgaacttccc gagatacgac acaatgtccc aggtgcagat gccgcacgcc
     3781 tcgatctgca cgagcacctc gtccggtccg gggtccggta ctttcaccgg ccgaacttcc
     3841 agcgaccgct cgccgaggta ccacagtcct tcgctcgcca caggcatcgt tcctacggta
     3901 tagcatagaa atggccgaaa ggatggtgta cgctgaggga gtctgagtcc gcaggaggta
     3961 gatatgccgg tgatcgcggt ggtgggcgcg aactggggtg atgaaggcaa gggcaagatc
     4021 accgactacc tggcccggga ggcagacttc gtcgtccgat accagggcgg aaacaacgcg
     4081 gggcatacca tcatcaacga gtacgggaag ttcgcgcttc acctgctacc ctccggagtc
     4141 ttctatccgc acgtcgtgaa cgtcctgggc cccggcgttg ccgtaaacct ggcggcgttt
     4201 ctgaccgagg tggacaagct cgtggagaga ggcgtaccac ggcctgccgt ccgtgtcagc
     4261 gaacgcgccc aggcgctcct tccctaccac atccagttcg atgcgctgga agaagagcgg
     4321 ctgggaaagg agagctttgg ctccaccaag tccgggatcg ctccgttcta cgcggacaag
     4381 tacctgaaga tcgggcttca gctcaacgag ctcttcgacg aggagctcag ccgcaaccgc
     4441 ctcgccaagg ccttggccca gaagaacgtc ctgctggaga agctgtacgg caagtcaccg
     4501 gtgtcgatcg acgagatgat gagccagatc gccgggcttc gtgatcgcgt cgcccccttc
     4561 ctctcggata ccacgacgct tctgctggac gcggtcgaat ccggcaagac catcctcgta
     4621 gagggtcagc ttgggacctt gcgggatccc gatcacggca tctacccgta ccccacttcc
     4681 tcttctccgc tggccgggtt cgccaccgta ggtgctgggg ttcccccttg gtcgatctcc
     4741 cgcgtcgtgg cggtgaccaa ggcctattcc agctgcgtgg gtgcaggacc ctttgtcacc
     4801 gagctgcacg gggaggaagc ggagacgctt cgcaagcggg gtggggatgc cggagagtac
     4861 ggcgccacca cgggacggcc gcgcagggtt ggatggttcg attgcgtggc aaccgcctat
     4921 ggctgtcggg tgcagggcgc tacggaggct gccatcacga acctggatgt actgggctac
     4981 cgggacgaga tcctggtctg cacggcatac gatctggacg gaacgaccag cggggcattc
     5041 ccggtctcga ggctcctggc ccggtccaag cccgtctacg cttcgctccc gggttggaag
     5101 tgcgatatct cgtcagctcg gaagttcgag gacctgccag agagggcccg agcctatgtc
     5161 cggttcattg aggaacgcat tcgcacgccc atccgctgga tctcgattgg accgcgaaga
     5221 gagcaaacca tcgtacggta agggagcgtc cggttctacc ggacgcttcg tcccttacgc
     5281 agaagcatca cccccaggac cagcaagacc acgggaataa ccagccccag gatcgacacg
     5341 cttacggaga accgctccaa ggtccaacgg ctgaacggct cggagaagat ccgcagcacg
     5401 ccaatgaggc cggcaacgag caccacccag ccgatggtct tgcggttcac cagtgcgcgt
     5461 tcccagatgg ccttcatggc tggatcttct accagcggct cggtaggtcc ccgcttgccc
     5521 ttcgcctggt cggcgatcga cagagcatcg aaaacggcat aggaaaggaa cagcacgccc
     5581 agcgagggca ccaggtaagc ggtgagccag tacatgccgg tagcgtcaga gtacagcagc
     5641 acgaggaaga tcgagaggat gaggagcccc atgagcgcga accccttgcc gatgagtccc
     5701 agaaacatat gaccggcgcc agggaaaatg ctgaggagcc cggccgccag cttgcgccag
     5761 ccgggatgca cctgacccgc tccggaccgg gtgagcactt ccaccaggca gtccttgcac
     5821 cacagccgtc cctgcaggtc cacggcgcat tccgagcaga acggacgggc gcatcgggtg
     5881 caactccctt ccgcatcggc tcttgggtga ttggcgcact tcatctcagt ctccctccgt
     5941 tctgggagcc ggtgtccacc ggctctcgca ggattccttg cactgcccgc tgggcgccct
     6001 caaacgtgct cttgacgatt ccaccgctcg gctcggtcat cacggtccgc gctggccccg
     6061 ccagactcgg ataggcgacc cggggtatca ggaggctcag cgccaggaga agcgcggtca
     6121 gtacgagact gaacccgagc ctgcgaaacg ccagggctcg caggggccga tcacgcgagg
     6181 ccgggctgtg cgtggccggg cggcgtaagg catcccggta gaccgcctcc atgacgcggt
     6241 cttcgaaccg ggcgggggct ctcggctgtg gctctgccac aagggtacgc gtcaggagct
     6301 gcgccgagcg gatgcgcgcg gcgcaccgtg cgcacctcgc ttgatgggcc tggatggacg
     6361 ctgaaagccc ctgagatagt tgcccgtcaa cgaagcgctc cacaaggggg ttgacctgct
     6421 ggcatgtcat cgtcggcctc ccgtcggtat ctcccctggg gccgctggtt gccgctctgc
     6481 acgctccagg ctgtctcgga ggagctttcg ggcccggtag agccgggtct cgaccgtctt
     6541 gagcggtacg cccagcacct gggagatctg ttcataggac agcttctgat agtagagcat
     6601 tacgacgacg gtccggtaac gctcatccag ggtatccaga cactgccgca cgcggctcct
     6661 cgcttcggcg gatagaagtc cgtcctcgac gtccacgcga tcgtcggcca ccaggtccgc
     6721 gcggtccagc gtcactccgt ccagacgctt gcgccgacga agagaatcgg tacagaggtt
     6781 gaacgtgatg cgatagagcc acgttgagaa cctggcctcc cccttgaacg agctcaggcc
     6841 ccggaaggcc ctgaggaaca cctcctgggc cgtgtcctcc gcgtcaccag ggctgcccag
     6901 gaaacggagc gccattgcgt acaccgggtc ctggtaccgg cgcaccagct ccgcgaagtg
     6961 acgcgtctgg ccgccttgta cccgctcgat gatctcccca tcggtcatct gctgaacggg
     7021 acctccccga ggtcaggcca ccacggccac actacccgcg gctgccggcg gctgtcaaaa
     7081 gattggacga atcccccgga gaaaaccctt caggggaccg tgggctggtt ctggttagag
     7141 gggttgggaa atgggcggga agcgagggcc cggctactcg accccgcaga gacccgaccc
     7201 gcacttgccg aggtcacaac cgcactctcc ggtgtgacag gccgggatgg cgtccgcggg
     7261 ccgtttcatc tgcgcggaga aaaggctgag ccgctttacg agcttctcgc tatcgcagct
     7321 cgggcaggcc acccgggtgt ccgaccgcac cagaagctcg aactggctct tgcactcctt
     7381 gcactcgtac tcgaatatcg gcatggccta ctcccgacgc tgttcgcgtc catcccgcca
     7441 gatcacgcgg atcctcttgt agagcccgtc accctcgctc accgtctcga tgtcgccgta
     7501 acgggcgagc gaggtgtgga tgagccgtcg ctcgaacgga ttgagcggct ccaggagccg
     7561 cgacgaccgg ctcttgcgga cctgctcggc gacgtggttc gccatccgga tcaggcttcg
     7621 ctcccggcgg ctgcggtagt cctgggtgtc cagaacgatt cgcgtcgaat cgccgcccat
     7681 gcgcccaacg gcgagattca cgatgaactg gagggcctcc agggtcttcc cttgcttgcc
     7741 aatcaggatg cccgatccgt cgctctcgat gttcaccccg agccttccgt cctcacggaa
     7801 gttcaccgcc acctcggccg gaaaacccat ccgctccagg agcccgtggg tgaagtcgag
     7861 aaccttgtgc tcgaactcgc cgtccggtgg gaggttttcg gagcgcggac gcgtggaaac
     7921 gggagccccc cggacgatcc gctcctccat ctcctggtcc tggatctgca ggtttgcctc
     7981 ttcctggtct tccgccaggt ggacgcggat cttgaccttg cccccgcgga agaggatccc
     8041 cgggcgcttc gcctccacga tctcgaagtc gatctcgttt cggtccagcc ccagctcctc
     8101 aatagcccgg tctatcgctt ccttctcgtt cttgccttcg aactctttca cgatcatctg
     8161 cgactccttg gggcgctcac cggcctgatt tctggctctg cttgccgccg gggcgagccg
     8221 gcacaacggt cagcgcgtcc ttcttcttgc gaagagcatt gatgtacagc tgctggaaga
     8281 tggaaaggac gttctggacc gtccagtaca ggacgaggcc ggagggcatg tcgtacagga
     8341 tgaagaggaa cacaatgggc agcgcgtagg tgagcagctt catttgcgcg cccgtctggg
     8401 atggatccgt gggctgggtg aactttccct gcaggagctg cgtggccacc atgaccagcg
     8461 gcagggctcg gagggcggtc caccccagga gcgggatggt gaacgggaag ttcacgatgg
     8521 cctcgggaac ggagaggtcg gggatccagc ccgggatgaa cagggcgccg cgaagctcga
     8581 agtgggtatt cagaaggttg tagaggg
//
LOCUS       DASRZS010000370         6996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4438986, whole genome shotgun sequence.
ACCESSION   DASRZS010000370 DASRZS010000000
VERSION     DASRZS010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6996
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4438986"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFH83_08965"
     CDS             <1..203
                     /locus_tag="VFH83_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486536.1"
                     /translation="ASSLKARFPEIASFEPMKGRPMKSYFVLPKALYTDAGQMGKVLQ
                     EAAVYCRSLPAKAAKPRKPRKP"
     gene            complement(521..1432)
                     /locus_tag="VFH83_08970"
     CDS             complement(521..1432)
                     /locus_tag="VFH83_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486537.1"
                     /translation="MSSRAVLLSAGILCAALLAAPRALGDSSNQSWLLFEQGNSAASQ
                     KDYGNAMELYKEAVQAAGIFPEAEAAIGDVYLEEGEAALAQRQYQKAYDERKSLYIPD
                     QQYGIMYKLANLFEIQQAYNQMEAELGQIVADDKRFQETSTQHLRTQVEKNFVEKGLD
                     RVLMLYDFEDSFSAPAHSKLGWFYYRTGRFSQSVSHLLYSVVYRSSRIRNLLRERNST
                     YEFTDASALLASIEQSADLRSYAQSAGLYKDLYYLAGSMFANGYPRHATALWSLLKAC
                     PSAGQYQDLAVRQLKKPFMEPLLTVTR"
     gene            complement(1504..2130)
                     /locus_tag="VFH83_08975"
     CDS             complement(1504..2130)
                     /locus_tag="VFH83_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486538.1"
                     /translation="MRKSAVVRIAVLCLVMVALTSCIGIDSRLTIRDNGSGTLLLTYR
                     VSQIIADLGMAAGTGSVVPLPVTRADFDRALSSSGGKVRLTRFDRSEDARDITIHAEL
                     AFDSVDALAAVDSFQDADLKWSASGSQHQFSQVVAKAPRQPLSDDSKRMLDAFFEGYE
                     LSFSVQAPRPIQNATLGTLSTDRMTLSYKTSIKDAVTVSQDLVMSAQW"
     gene            complement(2243..2572)
                     /locus_tag="VFH83_08980"
     CDS             complement(2243..2572)
                     /locus_tag="VFH83_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486539.1"
                     /translation="MTKSDLARMFQRTQSTIHHWLNTGQVSHRNLMKIRSSYYFLHDA
                     RDPHQSERKCERCGKWLPLREFRQGKAICHSCENSRTLQYYWAHREEQLTKRKAKNWY
                     NRRIANS"
     gene            complement(2719..4272)
                     /gene="guaA"
                     /locus_tag="VFH83_08985"
     CDS             complement(2719..4272)
                     /gene="guaA"
                     /locus_tag="VFH83_08985"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET6486540.1"
                     /translation="MDRILILDFGGQSTQLIGRRIRQLGIYSEIAPGDAKLEDLPLAG
                     TKGIILSGSGFSVYEPGAPVLDTRLYEQGLPLLGICYGIQRMSADMGGRVESLGKKEF
                     GRAPVNLTRSSALFGRVPGKSFVSWMSHGDSVTGAPRGFEVIGVSESGIPSAFANEAR
                     RMWGVQFHPEASHCEHGMDILEAFASDICGAAREWDMRRFFEAERQALVSRIGSRRVV
                     LLISGGVDSCVVAALLLKSLPPDAVHLMYIDTGLMRLGESEEVRETLAGFGARHVHVV
                     DAAERFYAALAGVEDPEQKRRIIGDLFIKVQQEELGKLGIGEAFLAQGTLYTDLIESG
                     KGVGSKAAVIKTHHNVRSPLVEAARARGMVVEPLSSLYKDEVRALGRELGLPERIVSR
                     HPFPGPGLAVRILGEVTRQKCDILRRADSLFISELRARGLYDQIWQAFCVLLPIRSVG
                     VSGDARRYGHVLALRAVASVDAITADVYPMSMGDLLEISSLITNRVPEIGRVVYDVSS
                     KPPATIEWE"
     gene            complement(4272..5780)
                     /locus_tag="VFH83_08990"
     CDS             complement(4272..5780)
                     /locus_tag="VFH83_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HET6486541.1"
                     /translation="MESRGIFLNLSPLDHRYSLSSPELYRSLSAILGEEAAVRYCARV
                     EAALLKALLRHTATPGQPTAVDSAALVDSIDEAAARIDPAQVYEEERKTRHNIRALVN
                     VMKPLLPPPVRPLLHLGATSVDILDTAASLRYRDALRTVVLPLLCDLETELVRRCREE
                     ADTPQIGRTHGQHAVPLTFGFALAEYVSRLGESISRIAKAVGDLRGKLAGAVGAYNAT
                     SLLVKDPLALERDVLGSLGLEPSEHSTQLVEPEYLLRLLLEMNVAFGVIANLADDLRN
                     LQRTEIDEVREEFASTQVGSSTMPQKRNPWNSEHVKSLWKAFAPRVMTFFMDQISDHQ
                     RDLTNSASSRFVSEYVAGFSLATERMLSVVKSLRVDRARMAANMGRTGDLAFSEAAYV
                     LVALGGDAEAHERIRTATLRAEQGQARLVDVLREDPRVWEMIRQRLAATVGVEPEAFF
                     SDPSRYSGRAAERAREIAHSHAVRIEALRRELAASGSAPGGPTPPAGERAGN"
     gene            complement(5786..>6996)
                     /locus_tag="VFH83_08995"
     CDS             complement(5786..>6996)
                     /locus_tag="VFH83_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6486542.1"
                     /translation="RRRESAEGLYIPGLVVDGAGMIVLAIAALGQDSMVGALASLFVV
                     EGACVAGSLLASLRTTVRECVLATTVSVLVAAGMATDALLRFPGPISLAFLGVPFINA
                     LAGTLAAICCNRYSSALKENLVTEDLLRASRRLKMTMDIVSASIFNLHQLINKLAEIS
                     ATVSVGARNQAAGIEEVSSAAEKLQGAMESISRSTERTASIIGTTARFAESGNTIVQK
                     VIEEILGIHEVVDKMVTALARINDIADQTNLLALNAAIEASREGDEESGFSVVAEEVR
                     KLAEKSAETASEVSKWVRQIETVIERGGESSREAGKIFDTISQDLGASAGFIHDLFRS
                     VKEQLGANRAVTGTIEGIGSVVEDNRFSAEAVTRIVGDLKKEMLKLESLVGDKVQEAE
                     KLYRRVERAP"
BASE COUNT         1227 a         2350 c         2194 g         1225 t
ORIGIN      
        1 gcgcatcttc cttgaaggcc agatttcccg agatcgcaag tttcgagccc atgaaagggc
       61 gacccatgaa gagctacttc gttctcccta aggcgctgta caccgacgca ggccagatgg
      121 ggaaggtcct gcaggaagcg gccgtttact gccgcagcct gcctgccaaa gccgcaaaac
      181 cccgcaagcc gcgcaaacca tagctctgcc gagcctcaca ggcctccgcc gaggccccat
      241 tttcacctgc accgcaactt ccgataatca atattctgtc tatcattgga tgatatccaa
      301 acaccctcca gttgcggcat gtcgcacaag ctcccagagg cacgcgccgc agtagtccaa
      361 cgaaccgccc tgccaaccaa ggatcgtttc gcgtgcacaa cgcaattgag ctgccgctcg
      421 ggacgactca aggcgagctt aggcaatcct cgagcacttg ggcggcacgc gctccggcgc
      481 ctgcttcagc gccagccagt tgcccgcacc tgccggcagg ctacctggtc acagtcaaca
      541 gcggctccat gaaaggcttc ttgagttgcc gaaccgccag gtcctggtac tgccctgccg
      601 aaggacacgc cttcagcagt gaccagagcg cggtggcgtg ccgcgggtac ccgttggcga
      661 acatggagcc cgccaggtag taaaggtcct tgtagaggcc cgcagactgg gcgtaagagc
      721 gcaggtctgc cgactgctcg atcgacgcaa ggagagccga ggcgtccgtg aactcgtacg
      781 tactgttacg ttctctcaag aggttgcgaa ttcggctgct tctgtagaca accgaataaa
      841 gcaggtgaga cacggactga gaaaaccttc ccgtgcggta gtagaaccac ccgagcttgg
      901 agtgcgcggg ggccgagaaa gaatcctcga agtcatagag cataagcacg cggtccaggc
      961 ccttctccac gaagttcttc tccacctgcg tcctcaggtg ttgggtggac gtttcctgga
     1021 accgtttgtc gtcggccacg atctgcccca gctcggcttc catctggtta taagcctgct
     1081 gaatctcaaa gaggtttgcg agcttgtaca ttattccgta ctgctggtcc ggaatgtaca
     1141 aggactttct ctcatcgtat gctttttgat actgccgctg ggccagggct gcttcacctt
     1201 cctcgagata gacatcgcca atcgctgctt ccgcctcggg gaagatacca gccgcctgga
     1261 ccgcctcctt gtacagctcc atggcattcc cgtagtcctt ctgggacgcc gcgctgttgc
     1321 cctgctcgaa gaggagccat gactggttgg agctgtcgcc aagagcacgg ggcgcggcca
     1381 ggagtgctgc gcacaagatg ccagccgaca gcagaacggc ccttgagctc atcgccatga
     1441 tgattcgagc tttgcagccc ggcatgacgc tacttcaacc tccgcgctgc cgacacggca
     1501 gccctaccac tgggcgctca tcacgaggtc ctgcgaaacg gtcaccgcgt ccttgatgct
     1561 cgtcttgtag ctgagcgtca tgcggtccgt ggagagcgtg cccagggtgg cgttctggat
     1621 ggggcgcggg gcctgcacgg agaaggacag ctcgtacccc tcgaagaacg catccagcat
     1681 ccgcttgctg tcgtcactga gcggctggcg cggcgccttg gccaccacct gggagaactg
     1741 gtgctgggac ccgctggcgc tccacttcag gtccgcgtcc tggaaggagt ccaccgcggc
     1801 cagggcatcc acggagtcga aggcgagctc cgcgtggatg gtgatgtccc gggcgtcctc
     1861 cgaccggtcg aaacgcgtaa gcctcacctt ccctccgctg gaagagagcg cgcggtcaaa
     1921 gtccgcccgc gtcaccggaa gcggcaccac cgaaccggtc cccgccgcca tccccagatc
     1981 cgcaatgatc tgggagacgc ggtacgtgag cagcagagtt ccggagccgt tgtcacggat
     2041 ggtcagtcgg ctgtcgatgc cgatacacga ggtgagcgcg accatcacga ggcacaggac
     2101 agcaatccga acaacggcac tctttcgcat gggccttcct ttgggagaac agtggcttga
     2161 gggtactata gcggaatcgg agcgccatgg ccagttgcgg caggatggag cgggggcgag
     2221 ttgtcctccc ccgcgaagaa tgtcaggagt tggcgatccg ccggttgtac cagttcttcg
     2281 ccttgcgctt ggtgagttgc tcttcccggt gcgcccagta gtactgcagc gtgcgcgagt
     2341 tctcacagga atggcagatg gcctttccct ggcggaactc cctcagcggc agccactttc
     2401 cgcagcgctc gcactttctc tcactctggt gggggtcccg ggcatcgtgc aggaagtagt
     2461 agctggatcg gatcttcatg aggttccgat gggagacctg gccggtgttc agccagtggt
     2521 ggatggtgga ctgggtgcgc tggaacatcc gagcgaggtc ggacttggtc atgccccagg
     2581 tcgacatcat ccaggtgagc atctcgcgaa ccggaggatc ctctggcaat tctcctggca
     2641 ttacgtatac ctcgctacgt tacgtatcgg caacgtacgg ccattctcta aagtgagggg
     2701 gaaaaaacgc cgatgagcct attcccactc gatcgtggcc ggcggcttgc tggacacgtc
     2761 gtacaccaca cgtccgatct cgggaacacg gttcgtaatg agcgatgata tctcgagcag
     2821 atcccccatg ctcataggat aaacatcagc cgtaatggcg tccaccgaag ccactgcccg
     2881 cagcgcgagc acgtgaccgt aacgccgcgc atcgccagac acgccgacgg accgtatcgg
     2941 cagaaggacg cagaacgcct gccagatctg gtcgtacagc ccccgggccc tcagctccga
     3001 gatgaaaagg gagtcggcgc gccgcaggat gtcgcacttc tgacgggtga cctcgcccag
     3061 gattctcacc gccagccccg gtccggggaa cggatgccgg gacacgatcc gctcgggaag
     3121 gcccagctca cggcccaggg cgcggacctc gtccttgtac agcgagctca gcggctccac
     3181 gaccatcccc cgggcccgcg ccgcctccac cagcggcgag cgcacgttat ggtgcgtctt
     3241 gatgacggca gccttgcttc ccacgccctt gcccgactcg atgaggtccg tgtagagggt
     3301 accctgggcc aggaacgctt cccctatccc cagctttccc agctcctcct gctggacctt
     3361 tatgaacagg tctccgatga tccgccgctt ctgctcggga tcctccaccc cggcgagcgc
     3421 ggcgtagaaa cgttccgcgg cgtccacgac gtggacgtgc cgggccccga aaccggccag
     3481 ggtctcccgc acttcctctg actcccccag gcgcatcaac ccggtgtcga tgtacatgag
     3541 atggacggcg tccggcggga ggctcttcaa caggagcgct gccaccacgc aggaatccac
     3601 gccgccggag atcagcagga ccacgcggcg cgagccgatc cgggagacca gcgcctggcg
     3661 ttcggcctcg aagaaccggc gcatgtccca ttccctggcg gcgccgcaga tgtccgaggc
     3721 gaacgcttcc aggatgtcca tcccgtgctc gcagtggctg gcctcgggat gaaactgcac
     3781 gccccacatc cggcgggcct cgttggcgaa cgcggacggg atgccgctct cggaaacgcc
     3841 gatcacctcg aagccccgcg gcgccccggt caccgagtca ccgtggctca tccaggacac
     3901 gaagctcttg ccgggaaccc ggccgaacag ggcggagctc cgcgtgaggt tcacgggcgc
     3961 ccggccgaac tccttcttcc ccagggactc cacccggccg cccatgtccg cgctcatgcg
     4021 ctggatcccg tagcagattc ccagcagggg cagtccctgc tcgtacagcc gcgtgtccag
     4081 cacgggcgcc ccgggctcgt acaccgagaa ccccgagccg gagaggatga tccctttggt
     4141 gcccgcgagc ggcaggtcct ccagcttcgc gtctccgggg gcgatctcgc tgtagatccc
     4201 gagctggcgt atgcgacgac cgatgagctg cgtgctctgg ccgccgaaat cgaggatgag
     4261 gatgcgatcc atcagttccc ggccctttct cctgccgggg gcgtcgggcc gccgggggca
     4321 ctccctgagg cggcaagctc ccggcgcagc gcctctatgc gcacggcgtg cgagtgcgcg
     4381 atctcccgcg cccgctccgc cgcccgtccg ctgtaccggg acgggtcgga gaaaaacgcc
     4441 tccggttcca ctcccacggt cgcggccagc cgctggcgaa tcatttccca cacccgcggg
     4501 tcctcccgca gcacgtccac caggcgggcc tggccctgct cggcccgcag cgtggcggtg
     4561 cggatacgct cgtgcgcctc cgcgtccccg ccgagcgcca cgagcacgta ggctgcctcc
     4621 gagaaggcca ggtctccggt cctgcccatg ttcgcggcca tccgggcgcg gtcgacgcgt
     4681 aacgacttca ccacggagag catccgttcc gtggcaaggg agaaacccgc cacgtattcc
     4741 gacacgaacc gggaggaagc ggagttcgtc aggtccctct ggtgatcgga gatctggtcc
     4801 atgaagaagg tcatcacccg tggcgcaaac gccttccaga ggcttttgac gtgctcggag
     4861 ttccacgggt tcctcttctg cggcatcgtg gaggacccga cctgcgtgga agcgaactcc
     4921 tcccgcacct cgtcgatctc cgtcctctgc aggtttcgaa ggtcgtccgc aaggttggcg
     4981 atgacgccaa aggctacgtt catctccagc aggaggcgca gcaggtactc cggttccacg
     5041 agctgggtag agtgctccga gggctccagg ccgaggctcc ccaggacgtc gcgctccagc
     5101 gccagcggat ccttcacgag gagggaggtg gcgttgtagg cccccacggc tccggccagc
     5161 ttgccccgca ggtccccgac tgccttcgca atacgggaga tgctctcccc gagccgggat
     5221 acgtactccg ccagcgcaaa gccgaacgtg agcggcaccg cgtgctggcc gtgcgtgcgg
     5281 ccgatctggg gggtgtcagc ttcctcccgg cagcggcgca ccagctccgt ctccaggtcg
     5341 cagagcagcg ggaggacaac ggtcctgagc gcatcccggt agcgcaggga ggcggcggtg
     5401 tccaggatgt ccacggaggt ggcccccagg tggagcaggg gacggaccgg cggcggcagc
     5461 aggggcttca tcacgttgac cagcgcccgg atgttgtgcc gggtcttccg ctcttcctcg
     5521 tagacctgcg cggggtcgat gcgggccgcc gcctcgtcta tggaatccac gagcgcggca
     5581 gagtccacgg cggtgggctg cccgggcgtg gccgtgtgac gcaggagagc cttcagcagc
     5641 gcggcttcca cccgcgcgca atagcggacg gctgcttcct ccccgagtat ggcgctgagg
     5701 gagcggtaca gctcggggct ggaaagggag tagcggtgat ccagcggaga gaggttgagg
     5761 aagatcccgc gtgattccat gggcctcagg gagccctttc cacgcgccgg tacagcttct
     5821 ccgcttcctg gaccttgtca ccgaccaggg attccagctt gagcatctcc ttcttcaggt
     5881 ccccgacgat tcgggtcacc gcctccgcgg agaagcggtt gtcctccacc acggacccga
     5941 tcccttcgat ggtgccggtc acggcccggt tggcgcccaa ctgctccttc accgagcgga
     6001 acaggtcgtg gatgaagccg gcgctcgccc cgagatcctg ggagatcgtg tcgaaaatct
     6061 tccccgcctc ccgggaggac tcccctcccc tctcgatgac cgtttcgatc tgccggaccc
     6121 acttgctcac ttcgctcgcg gtctccgctg acttctccgc cagcttgcgg acctcctccg
     6181 ccaccacgga gaaaccgctc tcttcgtccc cctcccggga tgcctcgatg gcggcattca
     6241 gcgccagcag gttcgtctgg tcggcgatgt cgttgatgcg cgccagcgcg gtgaccatct
     6301 tgtccacgac ctcgtggatg ccgaggatct cctcgatcac cttctgcacg atcgtgttcc
     6361 cgctttccgc aaagcgcgcc gtggtcccga tgatggacgc cgtgcgctcc gtggaacggg
     6421 agatgctctc catggccccc tgcagcttct ccgccgcgga gctgacctcc tcgatgccgg
     6481 cggcctggtt gcgcgctccc accgagaccg tggcggagat ctccgccagc ttgttgatga
     6541 gctggtgcag gttgaaaatg gaggcgctca cgatgtccat ggtcatcttc agccgccggc
     6601 tggcgcgaag caggtcttcc gtgaccaggt tctccttcag cgccgaggaa tagcggttgc
     6661 agcagatggc ggccagcgtg cccgcgaggg cgttgatgaa cgggaccccc aggaacgcga
     6721 gggagatggg gccgggaaag cgcagcaggg cgtccgtcgc catacccgcg gcgacgagca
     6781 cggagactgt cgtggcaagg acgcattcac ggacggtggt ccgcagggag gcgagcaggg
     6841 agccggcgac acaggccccc tccaccacga acagggaggc gagggccccc accattgaat
     6901 cctggccgag ggccgctatg gcgagcacga tcatgccggc gccatccacg accaggccgg
     6961 ggatgtacag gccctccgcc gactcccgcc ggcgat
//
LOCUS       DASRZS010000371         4532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5204967, whole genome shotgun sequence.
ACCESSION   DASRZS010000371 DASRZS010000000
VERSION     DASRZS010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5204967"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1836
                     /locus_tag="VFH83_09000"
     CDS             241..1836
                     /locus_tag="VFH83_09000"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET6486543.1"
                     /translation="MRARRNLPQSRPGEGAPRAEPALPRGLIRYLYVTACVSGAAVMI
                     VEILGARMLAPFVGTSHFVWTAQIAVTLAALACGYYVGGRWVDRSASLGRLYWALLIA
                     GGYLSLTVALREWVAYRFLSLPLPVASLLTSVFLFFVPLSLLAMTGPFVVRQVTRALA
                     QVGGAVGRLTAVSTIGSLLGTAIIGYVLIPVLPNSYSMYGTSVLLAAAAGGYLLGWGR
                     RASAAIGMAAVAIVGLAAGFWGIRAEGLRSAGVSELYRGNSSFGMLQVVQVNGSPYRL
                     WLNDYLIQNTYDTVQRKSTSMFTYMLEGLARAYSPQLDSALCIGMGIGIVPRELAERG
                     VRVDVVEINPAVVPIAKRYFDLDPGTFHLTIGDGRYYVNRSRTQYDAVLLDAFLGESM
                     PSHLMSREAFLAIRRVLKPQGVLVINTFVDFGSDSDYLGASLYKTLAGVFPSVIVHAA
                     KNANTLFVASPRPDLSMIHQPDFAEVHGDALADVREAFATTLQPDLGKGIVLTDDYNP
                     VEVYDAVNRERLRRALALSMRAP"
     gene            1922..3262
                     /gene="ffh"
                     /locus_tag="VFH83_09005"
     CDS             1922..3262
                     /gene="ffh"
                     /locus_tag="VFH83_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET6486544.1"
                     /translation="MLDKLSGRLTDLIRQIGGKAHISEKNIQDAINEIKIALLEADVN
                     LRVVRRFVNQATEEALGEKVLHSVTPGQQFVKILYDRMVALLGGETQSLALKGPDTQS
                     VILLAGLQGSGKTTTAAKLALRLKEQGRRPMLVAADLVRPAAVEQLAVLGEKAGVEVH
                     REDVKDPVQVVRGALARARREQLDTVIVDTSGRLQVDDSLMEELLRIRDASKPDEVLL
                     VADAMTGQNAVEIAQAFDAKVSLTGVILTKFDSDTRGGAALSVKSITGKPIKFVGVGE
                     KLDGLEPFHPDRIASRILGMGDVVSLVEKAQKTMEVGEALALQAKLATETFTLEDMLD
                     QYRRMRKMGSVRSLMEMIPGLQGSGAEDKIDESSLKREEAMILSMTRGERLNYRIIGP
                     PRRARIARGSGSSVFEVNRFLKKFEKTCLMMKKMSKNAKYQAQVLAQLGAGQGQ"
     gene            3281..3529
                     /gene="rpsP"
                     /locus_tag="VFH83_09010"
     CDS             3281..3529
                     /gene="rpsP"
                     /locus_tag="VFH83_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET6486545.1"
                     /translation="MSVRIRLKRLGSKKRPYYRIVVVDSRTPRDGRVLEEVGLYHPIE
                     PKEKQVSIKQERVKDWLAKGATPSETVRRLLDRAVVAG"
     gene            3580..3813
                     /locus_tag="VFH83_09015"
     CDS             3580..3813
                     /locus_tag="VFH83_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET6486546.1"
                     /translation="MEKDLVEYIAKTLVDDPTGVAVNVIEGEKSTILELKVSDSDIGK
                     VIGKHGRIAKAIRTLLGAASTKSGKRVVLEILD"
     gene            3813..4313
                     /gene="rimM"
                     /locus_tag="VFH83_09020"
     CDS             3813..4313
                     /gene="rimM"
                     /locus_tag="VFH83_09020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET6486547.1"
                     /translation="MDLLAVGVITATHGVGGELKVKSFSGEPQHIVALQEAIFRKGAA
                     ERRLRIEGARPQPPGVILKLEGMSTPETARQLIGYELWVPRRQAAPLASGEYYAADLC
                     RCTVWFGDERIGEVRSVWDGGPSQLLEVTGTDGKMRLVPFLEHFIREVDLAAGRITLR
                     EDEIVR"
     gene            4310..>4532
                     /locus_tag="VFH83_09025"
     CDS             4310..>4532
                     /locus_tag="VFH83_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET6486548.1"
                     /translation="MTFTVLTLFPGLFSGFLESSILARAIARGLVSVRLVDIRDFAAD
                     RHRVCDDAAYGGGPGMVMKPEPIAAALDAN"
BASE COUNT          777 a         1426 c         1553 g          776 t
ORIGIN      
        1 cggtgagcgt cgggcctggg gagcagaagg agatcgtctt caggctcctg gaagagcgac
       61 gagcggtcca gatcatcgac tccgatgtct ccgtggtggc tactccgtcg cttccaggcg
      121 acggcgggac cgcctctgca ccgccaaccc agtgacgagg gatcttgttc caccttcgtc
      181 gctgttgcta atctccttcc cggaggggca acctgagatg gcacgaaagg gagtccgata
      241 gtgcgcgcgc gcaggaatct gccgcagtcg cgccccggtg agggagctcc cagagcggag
      301 cctgcactcc ctcggggcct gatccgctac ctctatgtca ccgcctgcgt ctcgggcgcc
      361 gcggtcatga tcgtggagat cctgggcgcc cggatgctgg ccccgtttgt cggcacgtcg
      421 cacttcgtct ggaccgcgca gatcgcggta accctggcag cgctggcctg cggatactac
      481 gtgggcggcc gctgggtcga tcgctcggcc agcctgggcc gcctctactg ggcgcttctg
      541 attgccggcg ggtacctctc cctcacggtg gcgctccgcg agtgggtggc ctaccgcttc
      601 ctgtcgcttc cgttgccggt ggcctcgctc ctgacatcgg tatttctctt tttcgtcccg
      661 ctgagccttc ttgcgatgac gggaccgttc gtcgtccgac aggtgacgcg cgcgctggcc
      721 caggtcggcg gagccgtggg ccgattgacg gccgtcagca ccatcgggag cttgctgggc
      781 accgccatca tcggctacgt cctgatcccc gtccttccca actcctacag catgtacggc
      841 acgtccgtcc tcctggcggc ggctgccggc ggatacctcc tggggtgggg acgcagggca
      901 tccgcggcga tcggaatggc cgctgtcgcg attgtggggc tggccgccgg cttctggggc
      961 atccgcgccg agggattgcg cagcgcaggc gtgagcgagc tgtaccgagg caactccagc
     1021 ttcgggatgc tccaggttgt ccaggtgaac ggctctccct accggctgtg gctgaacgac
     1081 tatctgatcc agaacaccta cgatacggtc cagcggaaga gcacctcgat gttcacctac
     1141 atgctggagg gtctcgcccg cgcgtacagc ccccagctcg attccgcgtt gtgcatcgga
     1201 atgggaatcg gcatcgtccc ccgcgagctg gcggagcggg gcgtacgggt ggacgtggtg
     1261 gagatcaacc cggccgtggt gccgatcgcg aagcggtact tcgacctgga ccccgggacg
     1321 ttccacctca ccatcggtga cggacgctac tacgtgaacc ggtcccggac ccagtacgac
     1381 gcggtgctcc tggacgcatt cctcggagaa tcgatgccgt cccacctgat gagccgcgaa
     1441 gcgttcctgg cgatccgccg ggtcctgaag ccgcaaggcg tcctcgtgat caatacgttc
     1501 gttgacttcg gctccgacag cgactacctg ggcgcgtccc tgtacaagac cctggccggc
     1561 gtgttcccaa gcgtgatcgt acacgcggca aagaacgcga acaccctttt tgtggcttct
     1621 ccccgtccgg acctctccat gatccaccag ccggattttg ccgaggtcca cggggatgcg
     1681 ctggcggacg ttcgggaagc atttgccacg acgctccaac ccgacctcgg caaggggatc
     1741 gtcctgacgg acgattacaa ccccgtggaa gtctacgacg ccgtcaatcg cgagcgcctg
     1801 cgccgggctc tcgcgctctc catgcgcgcg ccctagccag gccagcgcgc gccttgacac
     1861 actcctcccg ttctctgtat atatgtgtgc ctgttttcag gaatctggta gacgaacgcc
     1921 catgctggac aagttaagcg gtagactcac ggatctcata cggcagatag gcggcaaggc
     1981 gcatatctcc gagaagaaca tccaggacgc gatcaacgag atcaagatcg cgctgctgga
     2041 ggcggacgtc aacctccgcg tggtgcgccg gttcgtgaac caggccaccg aggaggcctt
     2101 gggagagaaa gtcctccact cggtcacgcc ggggcagcag ttcgtcaaga tcctgtacga
     2161 ccggatggtt gcgctcctgg ggggagagac ccagtcgctg gccctcaagg gtccggacac
     2221 gcagtcggtg atcctcctgg cggggctgca gggctccggc aagacgacca cggccgccaa
     2281 gctggccctt cggctcaagg agcagggtcg gcggcccatg ctggttgccg cggacctggt
     2341 gcggccggcg gccgtggagc agctcgcggt gctgggagag aaggccggcg tcgaggtgca
     2401 tcgggaggac gtcaaggatc ccgtccaggt ggtccgcgga gccctggctc gcgcgcgccg
     2461 ggagcagctt gacacggtca tcgtggacac ctccggccgc ctccaggtgg atgattccct
     2521 catggaggag ctgctccgca tccgcgacgc ctccaagccg gatgaggtcc tgctggtggc
     2581 ggacgccatg accggccaga acgcggtgga gatcgcccag gcgttcgacg ccaaggtgtc
     2641 gctgaccggg gtgatcctca ccaagttcga ttcggatacg cgcggcggcg ccgccttgtc
     2701 cgtgaagagc attacgggca agcccatcaa gttcgtgggt gtcggagaga agctggacgg
     2761 cctggagccg ttccacccgg accgcatcgc ctcgcggatc ctcggcatgg gcgacgtggt
     2821 gagcctggtc gagaaggccc agaagaccat ggaggtgggg gaggccctcg ccctccaggc
     2881 caagctggcc acggagacct tcacgctgga ggacatgctg gatcagtacc gtcggatgcg
     2941 gaagatgggc agcgtgcgct cgttgatgga aatgatcccg ggtctgcagg gctcgggcgc
     3001 ggaggacaag atcgacgagt ccagcctcaa gcgcgaagag gccatgatcc tctcgatgac
     3061 ccgcggagag cggctgaact atcggatcat cgggcctccc cgtcgcgcgc ggatcgcccg
     3121 ggggagcggc tcttcggtgt tcgaggtgaa ccggttcttg aaaaagttcg agaagacgtg
     3181 tctcatgatg aagaagatgt cgaagaacgc gaagtaccag gcacaggttc tggcgcagct
     3241 cggcgccggc cagggccaat agacacagga ggacgagcca gtgagcgtgc gcatccggct
     3301 gaagagactg ggaagcaaga agcgccccta ctaccgcatc gtggtcgtgg attcccgaac
     3361 cccaagggac ggccgcgtgc tggaggaggt gggcctctac catcccatcg agccgaaaga
     3421 gaagcaggtc tccatcaagc aggagcgggt gaaggactgg ctggccaagg gcgctacgcc
     3481 ctcggagacg gtccgccgcc tgctggatcg cgccgtcgta gccgggtagt ccgcccgcgg
     3541 accccggggt ccggcttcgc gtcctccaag gagaagcccg tggagaaaga cctcgtcgaa
     3601 tacattgcaa aaaccctggt ggacgatccg acgggtgtgg ccgtgaacgt gatcgaaggg
     3661 gagaagtcca ccatcctcga gctcaaggtt tccgacagcg acatcggcaa ggtcatcggg
     3721 aagcacggcc gcatcgccaa ggccatccgg accctgttgg gcgccgcctc gaccaaatcg
     3781 ggcaaaaggg tggtgctcga gatcctcgac tgatggacct gcttgcggtc ggagtcatca
     3841 ccgcaacgca cggggtagga ggggagctca aggtaaagag tttttccgga gagccccagc
     3901 acatcgtggc gctccaggaa gcgatctttc gaaagggcgc ggcggagcgc cgactgcgca
     3961 tcgagggcgc gcggccgcag ccgccggggg tcatcctgaa gctggaaggg atgagcacgc
     4021 cggagaccgc gcgccagctc attgggtacg agctgtgggt cccgcgccgg caagcggcgc
     4081 ccctggcgtc cggggagtac tatgcggcgg acctttgccg ctgcacggta tggttcgggg
     4141 acgagaggat cggggaggtg cgctccgttt gggatggtgg accctcccag cttctcgagg
     4201 tcacggggac ggacgggaag atgcgcctgg tgccgttcct ggagcacttc atccgcgagg
     4261 tggacctggc cgcgggcagg atcacgctgc gggaggacga gatcgtgcga tgaccttcac
     4321 ggtcctcacc ctgttccccg ggttgttctc ggggttcctg gagagctcca tcctggcgcg
     4381 ggccatcgcc cgggggctcg tgagcgttcg actggtggac atccgggact tcgcggcgga
     4441 ccgccaccgg gtctgtgatg acgccgccta cggcggcggg ccgggtatgg tcatgaagcc
     4501 ggagcccata gccgcggcgc tggatgccaa cc
//
LOCUS       DASRZS010000372         3356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4439212, whole genome shotgun sequence.
ACCESSION   DASRZS010000372 DASRZS010000000
VERSION     DASRZS010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4439212"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFH83_09030"
     CDS             <1..261
                     /locus_tag="VFH83_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin"
                     /protein_id="HET6486549.1"
                     /translation="WTDNGEAEPGSVRRMLDHYLGEEEARGGLYFGGHRPVSDGYLLR
                     ADGRYVQIHDPQRFVVAHLPATGPIDLERDVREIDSRWLLSI"
     gene            277..1914
                     /locus_tag="VFH83_09035"
     CDS             277..1914
                     /locus_tag="VFH83_09035"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6486550.1"
                     /translation="MDKQPPRTIGKYELIGKIAQGGMGTLYKARHPTLDRVVLLKKLT
                     LRGGEQVVERFRREARLMMDFKDERIVQVYDHFKAGQHYFIVEEYVDGMSLDQLIRRE
                     RYLGNDAITVILYEVCKALKYAHDKQVIHRDIKPANILLSKQGEVKLADFGIATSREE
                     TEDGLTQDGTMLGTPAYVPPEQLDDARNVDIRADIYSLGVVLYEMLTGHTPYPGSFTA
                     ETIRLIHRGKYTAIRRLNPKASRLLARIARVCMRPKRRRRYQDLGQVLRILERRIKRR
                     DPASLRKAVQRVLNDEPIQDVFRARRWWAVWLGAAVTLAAVGAAGAWYAGTQGFWLDV
                     FAANRYGALVVAAQVDSVYKGSGQVFFEPVLYREQNGSLTALSSVGYALRENTAAGSG
                     STFLIESKRLFLPAGRYRLKASLENQLYWTSFVLSPRTDQRKVFSTADALHIVIRQPN
                     PAPQPLEVRYRAVDADTAQELADAQLSVFLFNAWLPWSVVRVSGLSTGRSYRFRVEHD
                     GYRPQIFDLAIKPYQTILTIEAHLARSSGAASSETSP"
     gene            1911..2801
                     /locus_tag="VFH83_09040"
     CDS             1911..2801
                     /locus_tag="VFH83_09040"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6486551.1"
                     /translation="MTTMVRRLILACLGLLGGLAAWPVAELILSFQGSFPSYLAFNAV
                     LGAATGALMGAAFGAAEGITSRVKTRIPNGMLLGAAVGCVGGAAGLLAGQAALWLIGG
                     LFLRSYHSFQWVVLPVSHAIGWAVLGVFVGAGEGARAVSPRKAAVGVLGGLVGGLVGG
                     FLLEYSRLVLPAMALSRLIGLVILGISIAFFYGLIEQGLAYGVLRILTGSLKGKEFLV
                     SQRRMRIGRARRNEIALPDYEDLAETQAQIRIKRGEAILMTLQKEPPMLVNERKVSER
                     ALKLGDVIRIGSARFFFRYE"
     gene            2811..>3356
                     /locus_tag="VFH83_09045"
     CDS             2811..>3356
                     /locus_tag="VFH83_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6486552.1"
                     /translation="MAKRSLLASSGVAALFALLMNAPIAVAITVSLSQLDSSSLLATQ
                     SVNAYVSVTDDAGEPVTTLPQSAFRILESPDGATFGPPRAITGFTPNAGASQGITFLL
                     LIDNSGSMYDTLDGKPTTALQAMRVTLAKAAVRSFLASMTSPYDRVGLVAFNTNYHVL
                     TGATTSRDRLSSLLEGITRPVP"
BASE COUNT          541 a         1131 c         1112 g          572 t
ORIGIN      
        1 tggaccgaca acggcgaggc ggaacccggc agcgtgcgca ggatgctcga ccactacctg
       61 ggagaggagg aggcccgggg cggcctctac ttcggcgggc accggcccgt ctccgacggg
      121 tacctccttc gggcggatgg gcggtacgtg cagatccacg atccgcagcg cttcgtggtg
      181 gcgcatcttc cggcgaccgg gcccatcgac ctcgagcggg acgtgcggga gatcgactcc
      241 cgctggctcc tgtcgatctg aaaggaaggt gaaaacgtgg acaagcagcc gccgcgcacc
      301 atcggcaagt acgagctgat cgggaagatc gcccagggag gcatgggcac gctgtacaag
      361 gcgcgtcacc ctaccctgga ccgggtcgtg ctcctgaaga agctgacgct tcggggcgga
      421 gagcaggtgg tggaacgctt ccgccgcgaa gcccggttga tgatggactt caaggacgag
      481 cgcatcgtcc aggtctacga tcatttcaag gcgggccagc actacttcat cgtggaggag
      541 tacgtcgacg gaatgtcgct ggaccagctc atccgccgcg agcgttactt gggcaacgac
      601 gccatcaccg tcatcctcta cgaggtctgc aaggcgctga agtacgcgca cgacaagcag
      661 gtcatccacc gcgacatcaa gccggccaac atcctcctgt ccaagcaagg tgaggtgaaa
      721 ctcgcggact tcggcatcgc cacctcccgg gaggaaacgg aggacggtct gacgcaggac
      781 gggaccatgc tgggcacccc ggcctacgtg ccgccggagc agctcgatga cgcgcgcaac
      841 gtggatatcc gcgcggacat ctactccctg ggggtggtgc tctacgagat gctcacgggc
      901 cacaccccgt accccggctc cttcaccgcg gagaccatcc gcctgatcca tcgagggaag
      961 tacaccgcga tccgccgcct gaaccccaag gcctcgcgtc tcctggcgcg gatcgcacgg
     1021 gtctgcatgc ggccgaagcg gcggcgccgc tatcaggacc tgggccaggt cctgaggatc
     1081 ctggagcggc ggatcaagcg ccgagacccg gcgtccctgc gcaaggccgt gcagcgggtt
     1141 ctgaacgacg agccgatcca ggacgtcttt cgcgcccggc gatggtgggc cgtgtggctg
     1201 ggcgcggcgg tgacgctggc cgccgtaggc gccgcgggag cctggtacgc ggggacccag
     1261 gggttctggc tggacgtgtt cgcggccaat cgttacgggg ccctcgtggt cgctgcccag
     1321 gtggacagcg tctacaaggg gtccggacag gtcttcttcg agccggtcct ctatcgcgag
     1381 cagaacggat cccttacggc gttgtcatcc gtcggttatg cgctgcgtga gaataccgct
     1441 gccggctccg gctccacctt cctcatcgaa tcgaagcggc tattccttcc cgccggccgc
     1501 taccgcttga aggcgagcct ggagaaccag ctttactgga ccagctttgt cctcagtccc
     1561 cggaccgacc agcgaaaggt gttctccacg gcggacgccc tgcatatcgt gatccgccag
     1621 ccgaacccgg ccccccagcc gctggaggtt cgctaccggg cggtggacgc ggatacggct
     1681 caggagctcg cggacgcgca gctttccgtg ttcctgttca acgcgtggct gccctggagc
     1741 gtcgtccgcg tgagcggcct ctcgaccggc cgcagctacc ggtttcgcgt cgagcacgac
     1801 ggctaccggc cgcagatctt cgatctcgcc atcaagccgt accagacgat cctgaccatc
     1861 gaggcgcatc tggcgcggtc ctccggtgcc gcctcttcgg agacctctcc atgactacca
     1921 tggttcgaag gctcatcctt gcctgcctcg gccttctggg aggcctggcc gcctggcccg
     1981 tggccgagct gatcctctcc ttccagggaa gcttcccttc ctaccttgcg ttcaacgcgg
     2041 tcctgggcgc ggctaccggc gctctgatgg gcgccgcttt tggcgccgcc gaggggatca
     2101 ccagccgcgt caagacgcga atccccaacg gaatgctcct gggcgcggcc gtcggatgcg
     2161 tcggcggcgc cgcggggctg ctggcgggcc aggcggcgct gtggctcatc ggcggcctgt
     2221 tcctgcggtc gtaccacagc ttccagtggg tggtcctccc ggtctcgcac gccatcggat
     2281 gggctgtgtt gggcgtgttc gtgggcgccg gggaaggagc gcgagcggtc tccccccgca
     2341 aagccgcggt gggcgtcctg ggcgggctgg tgggcggact ggtgggcggc ttcctgctgg
     2401 agtactcccg cctggtcctt ccggccatgg cgctctcccg cctcatcggt ctggtcatcc
     2461 tgggaatctc catagcgttc ttctacggcc tgatcgagca ggggctcgcg tacggggtgc
     2521 tgcggatcct cacggggtct ctcaagggca aggagttcct ggtcagccag cggcggatgc
     2581 ggatcggacg cgcgcgcagg aacgagattg ccctgccgga ctacgaggac ctggccgaga
     2641 cccaggccca gatccggatc aagcgggggg aggccatcct catgacactg cagaaggagc
     2701 cgccgatgct cgtcaacgag cggaaggtct cggagcgcgc cctcaagctc ggggacgtga
     2761 tccggatcgg ctccgcgcga ttcttcttcc gatacgagta ggggataccc atggccaaga
     2821 gatcgcttct ggcgagcagc ggcgtagcgg cgctctttgc gctcttaatg aacgcgccga
     2881 tcgccgtcgc catcaccgtg agcctttctc agctcgattc ctcttcgcta ttggccacgc
     2941 agagcgtgaa cgcctacgtc agtgtcacgg acgacgcggg tgagcccgtc acgactctcc
     3001 cgcaaagcgc gttcaggatc ctggagtcac cggatggggc cacgttcggt ccgccgcggg
     3061 cgatcacggg cttcactccc aacgcggggg cctcgcaagg aatcacgttc cttctcctga
     3121 tcgacaactc gggcagcatg tacgacaccc tggacggaaa gcccaccacg gccctccagg
     3181 ccatgcgcgt cacgctggcc aaggccgccg tccggagctt tctggccagc atgaccagcc
     3241 cctacgaccg ggtcggcctg gtggcgttca acacgaacta ccacgtcctc accggggcca
     3301 cgacaagccg cgaccggctc tcctccctgc tggaggggat cacgcgtccc gtgccg
//
LOCUS       DASRZS010000373         3351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2127374, whole genome shotgun sequence.
ACCESSION   DASRZS010000373 DASRZS010000000
VERSION     DASRZS010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2127374"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1967)
                     /gene="polX"
                     /locus_tag="VFH83_09050"
     CDS             complement(240..1967)
                     /gene="polX"
                     /locus_tag="VFH83_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HET6486553.1"
                     /translation="MNNREVSEAFSLIANLLEIKGEVVYVTLAYRKAAESLLSLAGDV
                     NQFWKEERLREIPGVGKSIAEKIDELLKAGSLGFLEDLKKEVPAGLADWLQVPGLGPK
                     KVALIWKELGITTLAELDAAARDGKLRKLPGLGEKSETQISRGIASLNRRTARVPLWR
                     AWPLAQQIAAALRKVPGVQRVEPAGSLRRMRPTVGDLDILVASADSGPVMEAFTTLPG
                     VMTVRGKGEMKSSIEFADGVRAQVWVHPPEQFGTALQYATGSKDHNVQLRQLALEKGL
                     SLSEHALSRTDGSGDVLCATEEEVYAALGLPYIPPELREDRGEVQAARAGTLPRLIEV
                     ADIRADLHAHTTWSDGKLSILELARAAMRRGLRVLALTDHSVGLGMVNGLSVERHAEQ
                     HAEIQAAQRDLGDRIRLLHGTEVEIKADGTLDYPDEFLATLDLVVASLHSSLRQDRSV
                     ATERLLRAVRNPHVDIIGHPTGRLIPDRPGADLDMDAVLAAAAQSGVALEINASPYRL
                     DLEDAYARRARDMGIPLSINTDAHSEADLAMLPFGVATARRAWLTAADVINTWEPDRL
                     LDWLGKRGR"
     gene            2121..3242
                     /locus_tag="VFH83_09055"
     CDS             2121..3242
                     /locus_tag="VFH83_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6486554.1"
                     /translation="MMFDRERYLSLLTFGHADRDMFVELFGPLVGLEDEWRAQGASPG
                     ECSLEDFDWDWVLLAGCGGTTQPRGRDTRVLLEETAEHILERDYLGRTVKLPKGKATI
                     PLPLDNPVKTMDDWLRVKHLYTFGEDRIDADAVERARKARDAGALVIASIPGAYDTPR
                     ELMGDEYACVCYYEQPELMQDVMDTIRQTSCQVLERVSRRVTIDQLSVSEDLAGKSGP
                     LVGPRQVDQFFRPYYRAVWDLLKSRGSTIFQIDSDGLFAPIIPNLVDAGINGMIPIEP
                     AAGMDTVALRGRYGKSFAMMGGIDKHVLRRSKEEIRRELEYKLQPSMRRGGTVFGLDH
                     RIPNGTPLENYRYYVDLGREMLGIPPRSPASKGWRRMAF"
BASE COUNT          560 a         1109 c         1095 g          587 t
ORIGIN      
        1 tgagcattct ttccctccgc gtgctggttc ttgtcctgtg gccatctctt tacaagagcc
       61 gtaccaagag caggagtcga gatgcaactg attccctgac aaagaaatgg agccacaccg
      121 atcggtggga acaggagagc gtttcttcga aagataagtt tacccgggga tggcctggcg
      181 ccggagaagt cctttctccg caaacacttg tgccgacccg taaagacttg tttcaggttt
      241 cagcggccgc gcttgcccag ccagtcgaga agccgatccg gctcccaggt gttgattaca
      301 tccgctgcgg tcagccaggc tcggcgggcg gttgccacgc cgaagggaag catggccagg
      361 tccgcctccg agtgcgcgtc cgtgttgatg ctgagaggga tgcccatgtc ccgagcccgc
      421 cgcgcgtagg cgtcttccag atcgaggcgg taggggcttg cgttgatctc cagcgcaacg
      481 ccggattggg ccgcggccgc cagcacggca tccatgtcga ggtccgcacc cggccggtcg
      541 ggaatgaggc ggccggtggg atgaccgatg atgtccacgt gcgggttgcg cacggcgcgc
      601 aatagccgct cggtggccac ggaccgatcc tgtcgaaggg aggagtgcag ggaggcgact
      661 acgaggtcca gggtcgccag gaactcatcc gggtagtcca gcgtgccgtc cgccttgatc
      721 tccacctccg tgccgtggag gaggcggatc cggtccccca gatccctctg ggccgcctgg
      781 atctccgcgt gttgttccgc gtggcgctcg acgctcagcc cattcaccat gccaagcccg
      841 acggagtgat ccgtcaatgc caggacccga aggccgcggc gcatggcggc cctggcgagc
      901 tccaggatgg agagcttccc gtcggaccac gtcgtgtgcg cgtgcagatc cgcccggatg
      961 tccgccacct cgatcagccg ggggagcgtg ccggcccggg cggcctggac ctcgcctcga
     1021 tcttcccgca actcgggcgg gatgtacggc aaaccgagcg ccgcgtagac ctcctcctcc
     1081 gtggcgcaca ggacgtcccc ggacccatcg gtccggctga gcgcgtgctc ggagagggac
     1141 agccctttct ccagcgcgag ctgccgaagc tgcacgttgt ggtctttcga tcccgtggca
     1201 tactggagcg cggtgccgaa ctgctccggt ggatggaccc agacctgggc gcgcaccccg
     1261 tccgcgaact cgatgctgga tttcatctcg cccttgcctc ggaccgtcat cactccgggc
     1321 agggtggtaa acgcctccat gaccggcccc gagtctgccg aggccaccag gatatcgagg
     1381 tccccgaccg tgggccgcat gcgccgcagg gatcccgctg gttccacccg ctgcaccccc
     1441 ggcaccttcc gcagcgccgc cgcgatctgc tgggcaagcg gccacgcgcg ccagagggga
     1501 acgcgagccg ttcggcgatt cagggaggca atgccgcggg agatctgggt ctcggacttc
     1561 tctccgaggc caggcagctt tcgcagcttc ccgtcgcggg ccgcggcgtc cagctccgcc
     1621 agggtggtga tccccagctc cttccagatc agcgccacct tcttgggccc caaccccggc
     1681 acctggagcc aatccgccag gcccgcgggc acctccttct tcagatcctc gaggaagccg
     1741 aggctgccgg ccttgaggag ctcgtcgatc ttctccgcga tggactttcc cacaccgggg
     1801 atctcacgaa gccgctcctc tttccagaac tggttcacgt caccggcaag ggagagaagg
     1861 ctctcggcgg ccttgcggta ggcaagggtc acatagacca cctctccttt gatctccaga
     1921 agattggcga tgagggagaa tgcctcggat acctctcggt tgttcacacg ggacctcttg
     1981 ctgccgccac ggacctgggg ccaagctact tcggctcggg ttgtcggtca aatgtgcggc
     2041 gagtccctgt gtcgaccggt cccgccgtat tgacagctat ggtagcatcg tccacaaaac
     2101 cgacgggaga acacaccgcg atgatgttcg atcgagaacg ctatctctcg ctcctgacct
     2161 ttggtcacgc cgaccgcgac atgttcgtcg agctcttcgg ccccctggtg ggtctggaag
     2221 acgagtggcg agcccagggc gcttctcccg gcgagtgctc cctggaggac ttcgattggg
     2281 actgggtgct gctggcgggc tgtgggggaa ccacgcagcc acgcgggaga gacacgcggg
     2341 tgctcctgga ggagacggcg gagcacatcc tggagcggga ctacctgggc cgcaccgtga
     2401 agctccccaa gggcaaggcc accattccgt tgccgctgga caacccggtg aagaccatgg
     2461 acgactggct tcgggtgaag cacctctaca cgttcgggga agaccgcatc gacgcggacg
     2521 cggtggagcg cgcccgaaag gcccgggatg ccggcgccct ggtcatcgcc agtatccccg
     2581 gagcctacga cacgccgcgc gagctgatgg gcgatgaata cgcctgcgtc tgctactacg
     2641 agcagcccga gctgatgcag gacgtcatgg acaccatccg ccagacctcc tgccaggtgc
     2701 tggagcgcgt gagccggcgc gtcaccatcg accagctctc cgtgagtgag gacctggcgg
     2761 gcaagtccgg accgctcgtg ggaccccggc aggtggacca gttctttcga ccgtactacc
     2821 gggcggtctg ggacctgctc aagagccgcg gatccacgat cttccagatc gacagcgacg
     2881 gcctcttcgc gcccatcatc ccgaacctgg tggacgccgg gatcaacggc atgatcccga
     2941 tcgagcccgc cgccggcatg gacaccgtgg cgctgcgggg gcgctacggc aagagcttcg
     3001 ccatgatggg cgggatcgac aagcacgtgc tgcggcggtc caaggaggag atccggcggg
     3061 agctggagta caagctgcag ccttccatgc gccgcggtgg gacggtcttc gggctggacc
     3121 atcggattcc caacggtacg ccgctcgaga actatcgcta ctacgtggac ctgggacgcg
     3181 agatgctggg catcccgccg cgcagccccg cctccaaggg atggcggcgg atggcttttt
     3241 aggtatcttt tagcgcacgg aaggtactcg atgatgattc gcatccggca tttcctggtc
     3301 cccctgttgt tctgcagtgc gcttctcccg gtctccgcgc tgggccggca c
//
LOCUS       DASRZS010000374         5124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_593833, whole genome shotgun sequence.
ACCESSION   DASRZS010000374 DASRZS010000000
VERSION     DASRZS010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593833"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1041)
                     /locus_tag="VFH83_09060"
     CDS             complement(136..1041)
                     /locus_tag="VFH83_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017689303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6486555.1"
                     /translation="MARKDLKGISSGSNALLNVFFAIYSALCIAPLLLVVAVSFTDEK
                     TVYREGYKYIPSKLSLAAYKFVFTNVDQVMHAYGVTILVTVVGTLASLLFTAMLAYVL
                     SRKDFRYRKALAFYVFFTILFSGGFVPWYVICVRVLHIKDTLWALILPYLINGWYVII
                     LRTFFASNIPDEILESARIDGAGELRTFFQIVTPLAIPGLATIGLFNVLTYWNDWWLP
                     LTLINNDRLYNLQYLMYKVELDIQYLKMARQSMGALALKELMSIPSETSRMAMAVIAI
                     GPIVLAYPFFQRYFVRGLTIGALKG"
     gene            complement(1051..1920)
                     /locus_tag="VFH83_09065"
     CDS             complement(1051..1920)
                     /locus_tag="VFH83_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015845206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6486556.1"
                     /translation="MALPGILLLFAFNYMPMFGLIIAFKNMNFTKGFFRSDWYGLKNF
                     QFFINTPYAYTITRNTILYNLVFIGLGNIFAVACAIALHELRNRRTAKVYQSILFLPY
                     FLSWVVVSYLVFSFLSVDMGLINKQILPVFHVPPIEWYGNTRYWPFILTFANLWKYTG
                     YNSVIYLATIVGILPEYFEAATIDGASKLQQIRKITIPLISPVIIILVLLGIGRVFFA
                     DFGLFYQVPKNTGALFDVTNVIDTYVYRTLINSGDLGMSSAAGLYQAVVGFILVLASN
                     LIVRRIDPSKALF"
     gene            complement(2068..3300)
                     /locus_tag="VFH83_09070"
     CDS             complement(2068..3300)
                     /locus_tag="VFH83_09070"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6486557.1"
                     /translation="MPGPGFEWIGQEEEKEVLEVLRGRWLFRYGDETSPVFKRKTKTL
                     EEMVQQGFGTRHALAVTSGTAALITALTALGIGPGDEVIVPGYTFIASMSSVIHARGV
                     PVLAEVDESLTLDPDDVERKITPRTRAIMPVHMLGNPCNLERLQAIAHKHKLPIVEDA
                     AQAFGGSYRGKRLGTIGKIGAYSFNIFKVINAGDGGMVVTDDDDLYLRAFGYHDQGHL
                     PSRSGVEIGKRSIIGQNYRMNELTAAVLVAQYRRLDVILQHLRALKGRFKKALAGLPG
                     VSFRTINDQAGECATLLTVLLPDARTAEKVAGALGTTTVSKSGWHVYSNMEQILGKMT
                     AAGRGCPFDCESYPCFQDYRPGMLPRTDAILARAINLSVGVVDRGLGAAFGVSPHATD
                     QEVESAAQKFQAAVREAI"
     gene            3499..4665
                     /locus_tag="VFH83_09075"
     CDS             3499..4665
                     /locus_tag="VFH83_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6486558.1"
                     /translation="MPTRSAIAQAAGLSTVSVTSLLSGLLARGLVARAGKAPSTGGRP
                     CVIYGPGPRIGYTIGLFLEAGVCYLVAIDASGAVLLEQRAPLALSEAPGDRGTEIANG
                     ISAQVSRLQADPTLQGRRLLAVGVTPPGMVDTRRGLWLHGLRLSGVEHLRLGTLLEEA
                     LGVPVLVEDGARCLAHLALTRLGRDQAGDLLLVYLGSGVGAGLAMDGELFRGHRGLAG
                     EIGHLIVEERGERCGCGNSGCLETVASVPAVLRRFQRLLDEGVISRLQEKELTLERIG
                     EAAACGDRLALNGLAQIGASLGEACGKSILLFNPHTLLISGPVASLGDYLRDALWARV
                     RQQVIPEMLEDLSLQFLPSRPGDEGMGAASLASQWFWDRRASDGRVPELEEVLS"
     gene            4662..>5124
                     /locus_tag="VFH83_09080"
     CDS             4662..>5124
                     /locus_tag="VFH83_09080"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET6486559.1"
                     /translation="MSGNDREVRFERLRPAQVNQERTRCPLVFLPIGPLEYHGPHMPV
                     GTDPLVATNLALETCRRMGKGVVMPTLFWGTERERPEWMVESLGLPKGSWVVGMDFPT
                     ALWKSHYYPEQIFAMVVAAQLEMLIAGGYRVIVLVNGHGATNHLLTLERLAK"
BASE COUNT          943 a         1520 c         1750 g          911 t
ORIGIN      
        1 ctcggtggtt ctggagcgga ctggagacgt cgaaggctgc cgagatcctg gaccagggag
       61 ggtgcgtcga gccctagcca cggatggcga gggaccgcgg agtccatgga gggacggaag
      121 gcggccggcg gccggctacc ccttgagggc tccgatcgtc agaccgcgga cgaagtagcg
      181 ctggaagaac ggatacgcca ggacgatggg gccaatggcg atcacggcca tggccatccg
      241 cgaggtttcc gacgggatgg acatcagctc cttcagcgcc agcgcgccca tggactgccg
      301 ggccatcttc aggtactgga tgtccagctc caccttgtac atgaggtact gcagattgta
      361 gagccggtcg ttgttgatca aggtcagcgg cagccaccag tcgttccagt aggtcagcac
      421 gttgaagagc ccgatggtgg cgagccccgg tatggccagc ggcgtcacga tctggaaaaa
      481 cgtgcgcagc tcgccggcgc cgtcgatgcg ggcggactcc aggatctcgt ccggaatgtt
      541 gctggcgaag aacgtcctca ggatgatgac gtaccacccg ttgatgaggt acggcaagat
      601 gagagcccag agggtgtcct tgatgtgcag cacgcgcacg cagatcacgt accacggcac
      661 aaagccgccg ctgaagagga tcgtgaaaaa gacgtagaac gccagggcct tgcggtagcg
      721 gaagtccttt cgggacagca cgtacgccag catggccgtg aacaggaggc tggccagggt
      781 ccccaccacc gtcaccagga tggtcacgcc gtaggcgtgc atgacctggt ccacgttcgt
      841 gaacacgaac ttgtacgcgg ccaggctcag cttggagggg atgtacttgt acccttcgcg
      901 gtagacggtc ttctcatcgg taaaagaaac ggccaccacc agcagcagcg gagcgatgca
      961 gagagcggaa tagatggcga agaaaacgtt gagcagcgcg ttcgagccgc tggaaatgcc
     1021 tttcaggtcc ttcctggcca cgatctcccc ctagaacaaa gctttgctcg gatcgatccg
     1081 ccggacgatg agattcgatg caagcaccag aatgaagccc acgacggcct ggtagaggcc
     1141 agcggcagag gacatcccca gatctcccga gttgatcagg gtgcggtaca cgtacgtgtc
     1201 gatgacgttg gtcacgtcga agagcgcccc ggtgttcttg gggacctggt agaagagccc
     1261 gaagtccgcg aagaacacgc ggccgatgcc cagcagcacc aggatgatga tgaccggtga
     1321 gatcagcggg atggtgatct ttcggatctg ctgcagcttg ctggccccgt cgatggtggc
     1381 cgcctcgaag tactccggca ggatccccac gatggtagcc agatagatca cgctgttgta
     1441 accggtgtat ttccacagat tggcgaaggt gaggatgaac ggccagtacc gcgtgttgcc
     1501 gtaccactca atgggtggca catggaagac cggcaggatc tgcttgttga tgagccccat
     1561 atcgacgctg aggaaggaaa acaccagata ggagacaacg acccaggaga ggaagtaggg
     1621 caggaacagg atgctctggt agacctttgc cgtcctgcgg ttgcgaagct cgtgcagcgc
     1681 gatggcgcag gcaaccgcga agatgttccc caggccgatg aacaccagat tgtagagaat
     1741 ggtgttgcgg gtgatggtgt acgcgtaggg cgtgttgatg aagaactgga agttcttcag
     1801 gccgtaccaa tcgcttcgga agaacccctt ggtgaagttc atgttcttga aggcgatgat
     1861 cagcccgaac atgggcatat agttgaacgc gaagaggagc aggatgcccg gcagggccat
     1921 gaggaacagg gtccggttct tgaacagctc gtggaagaaa ccacgcttct tcgagagagg
     1981 gggtcggatc agatcagcca agggaacccg ctttccataa ggatcgaacc atagtccgga
     2041 tctcactctc gctcgtcaag agcccgctca gatcgcctct cggacggccg cctgaaactt
     2101 ctgcgccgcg ctctccacct cctggtcggt ggcgtggggg ctcacgccga aggcggcgcc
     2161 caacccacgg tccaccactc ccacggacag gttgatggca cgcgccagga tggcatccgt
     2221 cctgggaagc atgccggggc ggtagtcctg gaagcaaggg tagctctcgc agtcgaacgg
     2281 gcagcctctt ccggccgcgg tcatctttcc caggatctgc tccatgttgc tgtacacgtg
     2341 ccagccgctc ttggagaccg tggtggtccc cagggcgcca gccaccttct ccgcggtcct
     2401 ggcatccggc aggagcacgg tcagaagcgt ggcgcattct cccgcctggt cgttgatcgt
     2461 gcggaagctc acccccggca ggccggccag ggctttcttg aaccggccct tcagcgcgcg
     2521 gaggtgctgg aggatgacat cgagcctgcg gtactgggcc acgagcacgg ccgcggtgag
     2581 ctcgttcatg cggtagttct ggccgatgat ggaccgtttc ccgatctcca cgccggaccg
     2641 gctgggcagg tggccctgat cgtgataccc gaaggcgcgc aggtacaggt catcgtcgtc
     2701 ggtgaccacc attccgccgt cgccggcgtt tatgaccttg aagatgttga acgagtacgc
     2761 gccgatcttg ccgatggtcc cgagcctctt gccccgatag gagccgccga acgcctgggc
     2821 cgcgtcctcc acgatgggga gcttgtgctt gtgggcgatg gcctgcagcc gctcgaggtt
     2881 gcacggattc ccgagcatgt gcacgggcat gatggcccgc gtacgaggcg tgatcttccg
     2941 ttccacgtcg tccgggtcca gggtcaggct ctcgtccact tccgcgagga ccggcacgcc
     3001 gcgagcgtgg atcacggaag acatggaggc aatgaaggtg tagccgggaa cgatgacctc
     3061 gtcccccggc ccgatcccca gcgcggtcag ggccgtgatg agagcggcgg tccccgaggt
     3121 cacggcaaga gcgtggcggg tgccgaaccc ctgctgtacc atttcctcga gggtctttgt
     3181 cttgcgcttg aagacggggc tggtctcgtc tccgtagcgg aacagccacc ggccgcggag
     3241 cacctcgagg acttccttct cttcctcttg tcctatccac tcaaaccctg gtcctggcat
     3301 tggcgactcc aacggggatc aacgttagaa gcaccgctat tatgccggga ttggcaccgg
     3361 tgtcaaggaa aaaacgccca aaacaggcat aagtcaatca cgacgttgac attcccgtca
     3421 gcttacgtta aaagtcgcac taatcctatg ttctcgcacg cggagattgg tgtcctgagg
     3481 gctgtccacc gtctagagat gccgacccga tcggccattg cgcaggccgc ggggctcagc
     3541 acggtgtcgg tgacgtcgct cctttcaggg cttctggccc gagggctggt ggctcgcgcc
     3601 ggcaaggcac cgagcaccgg cgggcgtccc tgcgtgatct atggtccggg tcccaggatc
     3661 ggctacacca tcggcctctt tctggaggcc ggcgtatgct acctggtggc gatcgatgcc
     3721 tcgggggccg tgcttctgga acagagggcg cctctggcgc tgtcggaggc gcccggggac
     3781 cgagggaccg agatcgccaa cgggatctcg gcgcaggttt cccgccttca ggcggatccg
     3841 acgctgcaag gacggcgact cctggcggtc ggcgtgacgc caccgggtat ggtcgacacc
     3901 aggcgcgggc tctggctcca cgggctgcgg ctctcggggg tggagcatct gcggctggga
     3961 acgctcctgg aagaggccct cggcgtcccc gtgctggtgg aggacggcgc ccggtgcctg
     4021 gcgcacctgg ccctgacccg gctgggcagg gaccaggccg gcgacctgct ccttgtgtac
     4081 cttgggtcgg gagtgggtgc ggggctggcc atggacgggg agctctttcg cggccatcgg
     4141 ggccttgccg gagagatcgg ccacctcatc gtggaagaac ggggcgagcg gtgcggatgc
     4201 ggaaactccg gctgcctgga gacggtggcc tccgtccccg cggtccttcg acggttccaa
     4261 cggctcctgg acgagggagt catttcccgg ctccaggaga aggagctgac cctggagcgg
     4321 atcggcgaag cggcggcctg cggagaccgg ctggcgctca atggcctggc ccagatcggg
     4381 gcgtccctgg gggaggcctg tggcaagagc atcctgctgt tcaaccctca cacgctcctg
     4441 atctccggcc cggtggcatc cctcggggac tatctgaggg acgcgctgtg ggcgcgcgtt
     4501 cgacagcagg tcatcccgga gatgctggag gacttgagcc tccagttcct gccgtcgcgg
     4561 ccgggagacg agggcatggg ggcggccagc ctggcatccc agtggttctg ggaccggcgg
     4621 gcgtcggacg gaagggtacc agagcttgag gaggtgcttt cgtgagcggg aacgatcgtg
     4681 aggttcgttt cgagcggctt cggccggcgc aggtcaatca ggagcgcacg cgctgtcccc
     4741 tggtattcct gcccatcggt ccgctggagt accacgggcc gcacatgccc gttggcacgg
     4801 atcccctggt ggccaccaac ctggcgctgg agacctgccg ccgaatgggc aagggcgtgg
     4861 tgatgccgac gctgttctgg ggaaccgagc gggagcggcc ggagtggatg gtggaaagcc
     4921 ttggccttcc gaagggaagc tgggtggtcg gcatggactt ccccacggcg ctctggaaga
     4981 gtcactacta tcccgagcag atcttcgcca tggtggtggc ggcgcagctc gagatgctga
     5041 tcgccggcgg ctaccgggtc atcgtgctgg tgaacggcca cggggccacg aaccatctcc
     5101 tgacgctgga gcggctggcc aaac
//
LOCUS       DASRZS010000375         8753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_977654, whole genome shotgun sequence.
ACCESSION   DASRZS010000375 DASRZS010000000
VERSION     DASRZS010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977654"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1089
                     /locus_tag="VFH83_09085"
     CDS             217..1089
                     /locus_tag="VFH83_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HET6486560.1"
                     /translation="MIINHNMSALFTERNLKFNNLAADKDMEKLASGEKINRAGDDAS
                     GLAVSEKMRSQIRGLRMAESNSENGVSFIQTAEGYLQETQDILQRLRELAVQAANGIY
                     STEDRMQIQVEVSQLVAEVNRIASHAQFNGMNMLTGRFARNTGENTVTASMWLQIGAN
                     MDQRTRVFIGTMTAQGLGVQGANGRASTATFISLSTPDNANAAIGIIDTALRLVSKQR
                     ADLGAYQNRLEYAMKGLSVGAENMQAAESLIRDTDMAAQMVEFVKDQILIQSSTAMLA
                     QANTKPQSVLQLFR"
     gene            1246..2592
                     /locus_tag="VFH83_09090"
     CDS             1246..2592
                     /locus_tag="VFH83_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET6486561.1"
                     /translation="MRSFATVFRSAGHQCHLVGGAVRDLLLGRDLTDFDIATDARPEV
                     VLGLFRRVIPTGIRHGTVTVLFRGTQFEVTTFRTDGDYSDGRRPDTVVFAPSIFDDLA
                     RRDFTINAMAYDLLADRLNDPHRGREDLKRGILRAIGDPAQRFHEDGLRPLRACRFAA
                     QLAFTIEPATRAAIPGALPILARVSAERVRDEILKILASTVPSVGLLAMQETGILGVV
                     LPELAEGIGVAQGDLHCYDVFHHSLYACDAAPRESQVLRLAALLHDVGKPRALGAGPG
                     GRPTFYGHEKISTAMSQEILTRLKLPGAVIKAVCHLVANHMFNYQEEWTDAAVRRLIS
                     KVGEASIGELMALRRADQIGMCRENAGRFPEGLSQFAQRVEEVQRGSRAFTLRDLAAS
                     GTDLMERLGLRPGPEVGILLNELLETVLEDPSLNERERLLQIAERMYHQRMAPGQR"
     gene            complement(2775..3491)
                     /gene="rnc"
                     /locus_tag="VFH83_09095"
                     /pseudo
     CDS             complement(2775..3491)
                     /gene="rnc"
                     /locus_tag="VFH83_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
     gene            complement(3507..3746)
                     /gene="acpP"
                     /locus_tag="VFH83_09100"
     CDS             complement(3507..3746)
                     /gene="acpP"
                     /locus_tag="VFH83_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET6486562.1"
                     /translation="MDELFEKMKKLIADRLEIDESKITLDSSFRQDLGADSLDTYELV
                     YAIEEELGISIPDEKANEFETVRDALEYIRSQVKK"
     gene            complement(3804..3989)
                     /gene="rpmF"
                     /locus_tag="VFH83_09105"
     CDS             complement(3804..3989)
                     /gene="rpmF"
                     /locus_tag="VFH83_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET6486563.1"
                     /translation="MALPKRRISRARGRQRRAVNLRLAVPTLVECSNCGNRMMPHRVC
                     PKCGFYRGKQILKPEEM"
     gene            complement(4138..4665)
                     /gene="coaD"
                     /locus_tag="VFH83_09110"
     CDS             complement(4138..4665)
                     /gene="coaD"
                     /locus_tag="VFH83_09110"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET6486564.1"
                     /translation="MFKVLLPGTYDPPTNGHLNILRRAARLFDAVEVVIAVNPGKTYL
                     FSEEERLVMMKTLAQGLPNVSVSTWNGLIVDFADRIGVHTIVRGVRALADFDYEFELS
                     LMNRSLNPRVETIFLPTDQKYSVIRSSAIKDIALFGGDISGMVPSIVATALHERLGEQ
                     RRQRGEPMEHREHPS"
     gene            4841..5578
                     /locus_tag="VFH83_09115"
     CDS             4841..5578
                     /locus_tag="VFH83_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486565.1"
                     /translation="MKASAGLPGILACASLLLIGCGGVVRQTVPPVAAPAAQYAAPVQ
                     HAQPVQEGTTTSTPVRAAQVDGHGVEQKATPAADPSASGASAATTQTGAQTRTADPGA
                     SGPQRGSAEDFVPTRELYDKTFGEVEDVVESLNRIIAADDYTRWLTYLTPEYISVTGD
                     PAFLAEASKSPVLLAKGTKLNTLRDYFDAVVVRSRTKVRLDSLSFIDEQHVKALTKMG
                     NSVVILYYLVRDDDSWKVGMWQNAKDQ"
     gene            5598..6323
                     /locus_tag="VFH83_09120"
     CDS             5598..6323
                     /locus_tag="VFH83_09120"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6486566.1"
                     /translation="MSRSAYARGAALVLFLTGAAFLLPAQAATAPAPKELTIEQLFLQ
                     SVEFQILREKAFSDDYDLKMSALDDLEKKIDTGATVGNDQQIEFVLEYLALEGSGHIT
                     REEGRVINNLPEVRRRSANLLGRLGTPEAQNALLRVLIIDQEPVVQSEAAYALGEIGS
                     NPDNEVLQALVFAYQHQDPAKPDNNFAYALTLAVEKLATKTAGGLKDPDAYRLLVNIA
                     QGAYLRTVRAKAVQVLGELQAAK"
     gene            complement(6359..6910)
                     /locus_tag="VFH83_09125"
     CDS             complement(6359..6910)
                     /locus_tag="VFH83_09125"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6486567.1"
                     /translation="MSLKFALLGLLAESPKYGYEIKRRFEGALGNVWSVSYGQLYPTL
                     RKLSEAGWVTKKTEPGKKAAEKNIYSITDRGRRKLDEWLLKPLRSAYKVKDEFTLRFL
                     FFSKLPPEQVLEYLKVQQKKTVLQRDSFQRTLVSLRDEIDYFLQAIIRKGIIHLEAEN
                     QWMEEVMGDLRASTRPPQTPEGL"
     gene            complement(6987..7382)
                     /locus_tag="VFH83_09130"
     CDS             complement(6987..7382)
                     /locus_tag="VFH83_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486568.1"
                     /translation="MAPQGRSSTPRSRPERPTGGPGRDPTVPPAPEAPRSYPDCPLCA
                     KPVKELASALTHRGSGRPAHFDCIVREIRESTEVGPQERLCYLGGGSFGVLEFRPSGG
                     PGAFVIKRRIQYEEKDPPAEWKKPLLLVR"
     gene            complement(7604..7744)
                     /locus_tag="VFH83_09135"
     CDS             complement(7604..7744)
                     /locus_tag="VFH83_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486569.1"
                     /translation="MVRIAKKTDPKKIAELKVKIHDADYLSQAIQKIAQDLTKNLEHG
                     ES"
     gene            complement(7744..8118)
                     /locus_tag="VFH83_09140"
     CDS             complement(7744..8118)
                     /locus_tag="VFH83_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET6486570.1"
                     /translation="MRRSPRSAGLGAAGEDQAALHLAAEGWTILARNYRSRYGEIDIV
                     ASREDEVAFVEVKAWRALSQGDLEYAVDYRKQGRVALTARRFLADNRRLADRRMRFDV
                     VFLGGDGAGVRHIEHAFDGGSD"
     gene            complement(8111..>8753)
                     /locus_tag="VFH83_09145"
     CDS             complement(8111..>8753)
                     /locus_tag="VFH83_09145"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HET6486571.1"
                     /translation="TGALLHDIGMVKVPKAILKKKEKLTAEELNQIHTHPVHSYRLIS
                     KELKYPEEIGLIALQHHERWDGQGYPRALRGEDINLSARVVAVADSYIAMINNRPHRN
                     SMIGYNAMKNLLNDNGRHFDPKILKVFLESMGIYPIGSIVQLNTSAIGQVVETHTEAP
                     LRPVVELIIDEFGNRRAEREIVDLLTRKGTFIVKAVDPKVLGPAPAPGDLPRA"
BASE COUNT         1518 a         2848 c         2814 g         1573 t
ORIGIN      
        1 tccctcgtcg agagtagcgc ggatcgcggc gggcggcaat cgcctcccca cggcgtgcgc
       61 tcgcaacgac ccggcccgtc ggacccgaaa accctcaagg tccctcggaa tcgaccgatc
      121 ctcaaaagtg agtcacccca aggggcggaa gatcctcccc tgggccgacc cacgcctgga
      181 cagggacgtc cgggctcgac gttcaaggag gaatgcatga tcatcaatca caacatgagc
      241 gccctcttca ccgagcgcaa cctcaagttc aacaacctgg cggcggacaa ggacatggaa
      301 aagctcgcct ccggggagaa gatcaaccgc gccggtgacg atgcatccgg gctcgccgtc
      361 tcggagaaga tgcgctcgca gatccgcggt ctgaggatgg cggagtccaa ctcggagaac
      421 ggcgtctcct tcatccagac ggcggaaggg taccttcagg aaacccagga catcctccag
      481 aggctgcggg agttggccgt ccaggccgcc aacgggatct attccaccga ggaccggatg
      541 cagatccagg tggaagtctc ccagctcgtg gcggaggtga accgcatcgc gtcccacgcc
      601 cagttcaacg gcatgaacat gctgaccggg cggttcgcgc ggaacaccgg ggagaacacc
      661 gtgaccgcca gcatgtggct gcagatcggc gccaacatgg accagcgcac ccgggtcttc
      721 atcggaacga tgaccgctca gggtctggga gtgcagggcg ccaacggtcg ggcttccacg
      781 gccaccttca tctccctgtc tacgccggac aacgcgaacg cggccatcgg gatcatcgac
      841 acggcgctgc ggctggtgag caagcagagg gcggacctgg gcgcctacca gaaccggctc
      901 gagtacgcca tgaagggcct ctcggtagga gcggagaaca tgcaggcggc ggagtccctg
      961 atccgggaca ccgacatggc ggctcagatg gtcgagttcg tgaaggacca gatcctgatc
     1021 cagtcctcga cggccatgct ggctcaggcc aacaccaaac cccagtcggt tctccagctc
     1081 ttccggtagt ccttcgaccg agcttcgatt gacggatgag gccccgatcc gagaggtcgg
     1141 ggctttttca ttgcggcccc cgggccgacc gatcggggct ttttcattga cactcctcgg
     1201 cggttcccgg gatacttgag ctcagctatg cgtatcccgt cggtggtgcg atccttcgct
     1261 accgtgttcc gctccgcggg tcatcagtgc cacttggtag gaggcgccgt ccgagatctt
     1321 ctcctgggcc gcgatctcac ggacttcgac attgccaccg acgcgcgacc ggaagtggtg
     1381 ctggggctgt ttcggcgggt catcccgacc gggattcgcc acgggaccgt gacggtcctc
     1441 ttccggggga cacagttcga agtgacgacg ttccgcaccg atggggacta ctccgacgga
     1501 agacggccgg acacggtcgt attcgcgccg tcgatcttcg acgacctggc tcgacgcgac
     1561 ttcacgatca acgccatggc ctacgatctt ctcgcggatc ggctgaacga tccccaccgg
     1621 ggcagggaag acctgaagcg gggcatcctg agagccatcg gcgaccctgc ccagcgcttc
     1681 cacgaggacg gcctccggcc cttacgcgcc tgccgcttcg ccgcccagct tgcctttacg
     1741 atcgagcccg ctacgcgggc cgccatccct ggcgcgctcc cgatcctggc ccgcgtctcc
     1801 gccgagcggg tgcgagacga gattctcaag atcctcgctt ctaccgtgcc gtccgtcgga
     1861 ctcctggcca tgcaagagac ggggattctg ggcgtggtac tgccggagct ggcggaaggg
     1921 ataggcgtgg cgcaggggga tctccactgt tacgacgtct tccaccattc cctctatgcc
     1981 tgtgacgcag cgccccggga gtctcaggtc cttcggctgg cggcccttct ccacgatgtc
     2041 ggaaagcccc gagcgttggg cgcgggaccg gggggaagac cgaccttcta cggacacgag
     2101 aagatctcca ccgccatgag ccaggagatc ctgacacggc tcaagctgcc gggagccgtg
     2161 atcaaggcgg tctgccacct cgtggcgaat catatgttca actatcagga ggaatggacc
     2221 gacgcggcgg tccggcgcct catctccaaa gtgggggaag cctctatcgg ggagctcatg
     2281 gccctgcgtc gggcggacca gatcggcatg tgtcgagaga acgccgggag gtttcccgag
     2341 ggcctgtcgc agttcgccca gcgcgtcgaa gaggtccagc gcggctcgcg tgcgttcacc
     2401 cttcgggacc ttgccgcttc gggaacggac ctcatggagc ggctgggtct gcggcccggg
     2461 ccggaggtgg ggatcctgct gaacgagctc ctggagacgg ttctggagga cccgtccctc
     2521 aacgagcgcg aacggctcct ccagatcgcg gaaaggatgt accaccagag aatggcgccg
     2581 ggacagcgct gagcccttgg tcggctcctg ggagtgcgga ccggtcagtg ctcctgcggg
     2641 tgggagcgct ccacgcgggt gccacgggcg ccgccgcgct ctccgccgcg ctcgtcgccg
     2701 cgcggggtgg ggcgctcgcc gcgtcgcgcg gcaccccgat ccgttccgtg cggcgtcgcg
     2761 cgctccgcca cgtgcatgcc ctcataggcc agacgggcgg cctcctgctc tgcctctttc
     2821 ttgttcttgc ccttgcccgc cccgaaagac cgttctccca cgtgcacctc gatccagaag
     2881 gtgcggtcat gatcgggccc cgtcttctga acgacgcggt atcggggata ggtcttcagt
     2941 cgcttctgca cgaactcctg gagcagggtc ttgtagtcct tggcgtggcg gttttccagg
     3001 accttgttga tctccggaat gagcatggtg tgcacgaagc ggcgcgcagg ctggaagccc
     3061 gagtccaggt agaaggcgcc gatgatcgct tccagacagt ccgcgaggat tgccttcttg
     3121 gaccgaccgc ccgagtactc ctctcctctt ccgatgagga tgtagttgtc caccctcagg
     3181 gcccgggcga tctcggagag gctggcctcg ctcaccacga agctcttgat ccgggccagc
     3241 tccccttccg gttgatcccg aagggtctca tagaggtact cgctgacgac caggccgagg
     3301 acggagtccc ccaggaactc cagcctctcg ttgttctccg cggcctcccc cgtctcgttg
     3361 gcgaaggagc gatgggtaaa tgcctgattg aggaactcca gctcccgaaa acggatccca
     3421 gcgtgtcgtt cgaagagctg gagctccttc ctgcgctccg gtttcagcgt tattcccggc
     3481 gcacgtctga tcttcctgaa caaaacctat ttctttacct gggagcgaat gtactccagg
     3541 gcgtctcgga ccgtctcgaa ctcattggct ttctcatcgg ggatgctgat gccgagctct
     3601 tcctcgatgg cgtatacgag ctcgtacgtg tcgaggctgt ctgccccgag gtcctggcgg
     3661 aaggaggagt caagggtgat cttggattcg tcgatctcca gccggtcggc aatcagtttc
     3721 ttcatcttct cgaacaactc gtccatggaa ctcaacctcc tgttagaagt gggatgaact
     3781 gatgtcagtg gggttgttct tcgtcacatt tcctcgggct tgaggatctg cttgccgcgg
     3841 tagaagccgc acttggggca gacacgatgc ggcatcatcc ggttgccgca gttgctgcac
     3901 tccacgaggg taggtacggc cagacgcaag ttgaccgccc gtcgctggcg cccccgcgcc
     3961 ctcgatattc ttctctttgg caaagccatg ttatgtcacc tcaaaaagtg gattgaaatg
     4021 gtaaagttac ggaaaactac accgagcgac cttgaatgtc aaggcactac gcgcttggcc
     4081 cacgccaggc gccggtcacc cgaacggcgg ggtccacgga cgctcggtgg cgcgggctca
     4141 cgacggatgc tcccggtgct ccatgggctc ccctcgctgt cggcgctgct cgcccagcct
     4201 ctcgtggagc gcggtcgcta cgatggaagg aaccatgccg gagatgtccc ccccgaagag
     4261 ggcgatgtcc ttgatggcgg aggagcggat aacggaatac ttctggtccg tcggcaggaa
     4321 gatcgtctcc acgcgcgggt tgagggaccg gttcatcagg gagagctcga actcgtagtc
     4381 gaaatccgcc agcgcccgaa cgccccgcac gatggtgtgc acgccgatgc ggtccgcgaa
     4441 atccacgatg agcccgttcc acgtgcttac gctgacgttc ggcagccctt gcgcaagggt
     4501 cttcatcatg accaggcgtt cctcttcgct gaagagatac gtcttgccgg ggttgacagc
     4561 gatcaccact tctacggcgt cgaagagccg cgccgcccgc cgcaggatgt tcaggtggcc
     4621 gttggtcggc ggatcatagg ttcctggcaa cagtaccttg aacatggtcg ccaggatagc
     4681 ggatggaccc tgagcccgca aggcccgggc tcggcgcccc cggctcggcg cccccggctg
     4741 ggcgcccccg gctgggcgcc cccggctcgg ccactcccgc ccccggctcg aagcgaaata
     4801 gttttgacaa aggcattgcc gaaagataga attataagcg atgaaggcga gtgcaggact
     4861 gcctgggatc cttgcctgcg catcgctact gctcatcgga tgcgggggcg tggtgcggca
     4921 gacggtgccg ccggtggccg cccccgcggc ccagtacgcc gcgccggtgc agcacgcgca
     4981 gccggtccag gaaggaacaa cgacatcgac tccggtacgc gccgcccagg tggacggcca
     5041 cggagtcgag cagaaggcaa cgccggccgc cgatccctct gccagcgggg cctccgcggc
     5101 gacgacgcag accggggccc agacgcgtac ggcagacccc ggggcatccg ggccgcagag
     5161 gggttccgcg gaagacttcg tcccgacgcg ggagctgtat gacaagacct ttggcgaggt
     5221 ggaggatgtg gtcgagtctc tcaaccggat cattgccgcg gatgactaca cccggtggct
     5281 cacctacctt acgccagagt acatcagcgt gaccggcgac cccgcgttcc tggcggaagc
     5341 ctccaagagt ccggtccttt tggcaaaggg aaccaagctg aatacccttc gggactactt
     5401 cgacgccgtg gtggtccgaa gccgaaccaa ggtgcgcctg gacagtctct cgttcatcga
     5461 cgagcagcac gtcaaggcgc tcaccaagat gggcaattcc gttgtgatcc tctactatct
     5521 cgttcgggac gacgattcct ggaaagtcgg catgtggcag aatgccaaag accaatagga
     5581 gaacgggagc aagaatcatg agcagaagcg catacgcgcg gggtgccgcg ctcgtccttt
     5641 tcctgacggg ggcggcattc ctcctgccgg cccaggccgc caccgccccg gcccccaagg
     5701 agctgaccat cgagcagctc ttcctgcaga gtgtcgagtt ccagatattg agagagaagg
     5761 cgttctccga cgactacgac ctgaagatga gcgcgctgga cgatctggag aagaagatag
     5821 acaccggtgc cacggtggga aacgatcagc agatcgagtt cgtgctcgag tacctggccc
     5881 tggaaggatc gggccacatc acgcgggaag agggccgagt gatcaacaac cttccggaag
     5941 tccgccgaag gtccgccaac ctccttggca ggctggggac cccggaggcg cagaacgcgc
     6001 tcctgcgcgt gctgatcatc gaccaggagc cggtggtcca gtccgaggcg gcgtacgcgt
     6061 tgggagagat cgggagcaac ccagacaacg aggtgctcca ggcgctggtc ttcgcctacc
     6121 aacaccagga tccggcgaag ccggacaaca atttcgccta tgcgcttacg ctcgccgtgg
     6181 agaagcttgc caccaagacc gccggcggcc tgaaggatcc ggacgcctat aggctgctgg
     6241 tgaacatcgc ccagggagcg tacctgagga ccgtacgggc caaggcggtg caggtgttgg
     6301 gagagctgca ggccgcgaag tagcggcggg agcccgcgcg gcgcccgcct tcgggcggct
     6361 acaggccttc cggtgtctgg ggaggcctgg tcgaggcccg aaggtcaccc atgacctcct
     6421 ccatccactg gttctcggcc tcgaggtgga tgatcccctt tcggatgatc gcctgaagga
     6481 agtagtcgat ctcgtcgcgc agcgacacca gcgtccgctg gaagctgtcc cgctggagca
     6541 cggtcttctt ctgctgcacc ttcagatact ccagcacctg ctccggaggg agcttgctga
     6601 agaacagaaa ccggagggtg aactcgtcct tcaccttgta ggcgctgcgc aagggcttga
     6661 ggagccattc atcgagcttg cggcggcctc ggtcggtaat ggagtagatg ttcttttccg
     6721 cggccttttt ccccggctcc gttttcttgg tgacccatcc ggcctcggag agcttgcgca
     6781 gcgtggggta gagctgcccg taggacacgg accacacgtt gcccagggca ccttcgaacc
     6841 tgcgcttgat ctcgtagccg tacttggggc tttcggcaag aagccccagg agggcaaact
     6901 tcaacgacat gccgctactc cctctgtccc ccggtcatgc cggacggttc cgcctgaccg
     6961 cgaaggccgg gcacccgcag ctccgctcag cggacgagca gcaggggctt tttccactcc
     7021 gcgggcggat ccttttcctc gtattggatg cgccgcttga tcacgaaggc tccgggaccg
     7081 ccgctggggc ggaactccag gacgccgaag ctgccgccgc ccaggtagca gaggcgctcc
     7141 tgcggtccga cctccgtgga ctctcggatc tcccgaacga tgcagtcgaa atgcgccggg
     7201 cggcccgagc cgcgatgggt aagcgcggag gccagctcct ttaccggctt ggcgcacagt
     7261 ggacagtccg ggtaggaacg gggcgcctcc ggcgcagggg gaacggttgg gtcgcgtccc
     7321 ggcccgccgg tggggcgttc cggacgtgac cgcggcgtag agctccgccc ttggggcgcc
     7381 acggaacccc gcccttgagg cggcgtggaa cctcgcccct cgggcggcgt agcgccctgc
     7441 gcgtcagtcg gcacggggcc ctgcccttcg cttccgcgcg cgccgggcgg cgtagagccc
     7501 cgccccgggg gcggcgtaga gccccgcccc ggggccgaag ggccgtctcc ctgctgctga
     7561 cgctgacggc gacggctgtt gcggcgccgg aaactccgag agctcatgac tccccgtgct
     7621 ccaggttctt ggtcagatcc tgggcgatct tctggatggc ctgcgacagg tagtccgcgt
     7681 catggatctt gaccttcagc tccgcgatct tcttcggatc ggtcttcttc gcgatccgta
     7741 ccactagtcg ctccccccgt caaaggcgtg ctcgatgtgc cgcacgccgg ccccgtcgcc
     7801 tcccaggaac accacgtcga agcgcatccg ccgatccgcc agccggcggt tgtccgccag
     7861 gaacctgcgg gcggtgagtg cgacccggcc ctgctttctg tagtccacgg cgtattcgag
     7921 gtccccctgg gaaagcgccc tccacgcctt cacttcgacg aacgccacct cgtcctcccg
     7981 ggaggcgacg atgtcgatct caccgtaccg agaccgatag ttgcgcgcaa ggatggtcca
     8041 gccctcggcg gcaagatgca gcgcggcctg gtcctccccc gccgcgccca ggcccgccga
     8101 ccttggagat ctacgcacgg ggcaggtctc ccggcgcggg cgccggacca aggaccttgg
     8161 ggtccaccgc cttgacgatg aacgtcccct tgcgggtcag aaggtccacg atctcgcgct
     8221 ccgcccgacg gtttccaaac tcgtcgatga tcagctccac cacggggcgc aagggtgcct
     8281 ccgtgtgggt ctccacgacc tggccgattg cggaggtatt gagctgaaca atgctgccga
     8341 tcggatagat ccccatgctc tccaggaaca ccttgaggat cttggggtcg aagtgcctcc
     8401 cgttgtcgtt caggaggttt ttcatggcgt tgtagccgat catcgagttt cggtgcggac
     8461 gattgttgat catggcgatg taggagtccg ccacggcgac gacccgggcg gagagattga
     8521 tatcctcccc ccgaagggcc cgggggtacc cctgcccgtc ccaccgttcg tggtgctgga
     8581 gcgcgatcaa tccgatctcc tcgggatact tcagctcctt ggagatgagg cggtacgagt
     8641 gcaccggatg ggtgtggatc tggttgagct cctccgcggt cagcttctcc ttcttcttga
     8701 ggatggcctt gggcaccttc accatgccga tgtcgtgcag gagggcgccg gtg
//
LOCUS       DASRZS010000376         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3284781, whole genome shotgun sequence.
ACCESSION   DASRZS010000376 DASRZS010000000
VERSION     DASRZS010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3284781"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VFH83_09150"
     CDS             <1..715
                     /locus_tag="VFH83_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET6486572.1"
                     /translation="RLAEDSDELFRKTVRLAAQMEVPVVNVLSGLPAGAPGDKSPSWV
                     TCPWPPHNLPMLDYQWNTVAIPYWSKAGAFAQEHGVKIAVEMHPGFLVYNVKSMLRLR
                     EAVGPVLGCNLDPSHLFWNGVDVPEAIRALGSAIHHVHGKDCVVDPINARVNGCNDPT
                     PYDRIPERSWTFRSLGYGHDAKMWRDIVSALRLVDYDYVISIEHEDAMMSSDEGLAKS
                     VALLKEVVMAEKPGKMFWA"
     gene            complement(794..>2392)
                     /locus_tag="VFH83_09155"
     CDS             complement(794..>2392)
                     /locus_tag="VFH83_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET6486573.1"
                     /translation="VDLGGTGKPDILILESEFKDGRLSRFANPGSSLKDPGLEGPWKE
                     QRLLDDVVYGHSLDVREAAGSVRVMIGEMEQGGWDAPYNHGARVLELRTTDRGASWTQ
                     DLIGKGEGTHQALWADVDGDGELEVAGKSAGFYYCSPVVQVWKRVSTPSPVARLRHRM
                     VDRDKPATGVEILPCDVDGDGRVDVICGKWWYRNPGIPGRGAWERREIPGVAQVINTH
                     DIDGDGRPELICIQAPRASKGDFYSDLSAQLCWLKAVDPIAGTWKLHEIGTGMGDWPH
                     GSLLGPFGRGGRLALAVSYHSAYNNPRHYPQLFEVPKDPTAGGWPVRTLAPIQYNEEM
                     LAGEVAGSGRADIIAGPWWLENLGNGEFTPHRYAPEDIVVGRMGLMKIAGSGRQDVVI
                     GQEVADWQRKIIPLSPLVWLERPANPREVPWPVHVIDWVFCAHSIGIGDVDGDGEDEV
                     VCGEHNPFWPYRSRCRLLVYKKADSGGRSWKKITLDDRFEHHVGAKVFPWAVGGEAGP
                     GRQAILSHGWKDSIYVHLWEPTGE"
BASE COUNT          371 a          857 c          761 g          403 t
ORIGIN      
        1 ccgcctggcc gaggactccg acgagctatt ccgcaagacc gtccggcttg cggcgcagat
       61 ggaagtcccg gtggtcaacg tgctctcagg gctgccggct ggagcgccgg gagacaagag
      121 cccgagctgg gtcacctgcc cctggccgcc gcacaatctc cccatgctgg actaccaatg
      181 gaacacggtc gccatcccgt actggagcaa ggcgggcgcc ttcgcccagg agcacggggt
      241 gaagatcgcc gtggagatgc acccgggctt cctggtctac aacgtgaaga gcatgctccg
      301 cctccgggag gccgtggggc cggtgctggg ctgcaatctc gatcccagcc atctcttctg
      361 gaacggggtc gacgtgcccg aggccatccg cgcgttggga agcgccatcc atcacgttca
      421 cggcaaggac tgtgtcgtgg atcccatcaa cgcgagggtg aacggctgca acgatccgac
      481 tccgtacgac cggatccccg agcgctcatg gacgttccgc agcctcggct acggtcacga
      541 cgcgaagatg tggcgggaca tcgtcagcgc gctgcgcctc gtggactacg actacgtgat
      601 ctccatcgaa cacgaggacg ccatgatgtc ctccgacgag ggcctggcca agagcgtggc
      661 cctgctgaag gaggtggtca tggcggagaa gccgggaaag atgttctggg cgtaggcgcc
      721 gggccggccg cgcccggcgg gcccggcctg tgcccgacgg ccagccgggc ggaccgcgcc
      781 ccacggcggc tcgtcactcg cctgtcggct cccacaggtg cacgtagatg ctgtctttcc
      841 agccgtggct caggattgcc tgccgccccg ggccggcctc tccgccgacg gcccacggga
      901 agaccttggc accgacgtga tgctcgaacc gatcgtccag cgtgatcttc ttccaggatc
      961 tgccaccgga gtccgccttc ttgtagacca gcagacggca tcggcttcgg tacggccaga
     1021 acgggttgtg ctccccgcag actacctcgt cctccccgtc cccatcgacg tctccgatgc
     1081 cgatggagtg ggcgcagaac acccagtcta tgacgtgcac cggccagggg acctcgcgcg
     1141 gattggcggg acgctccagc cagaccaggg gagaaagcgg gatgatcttc cgctgccagt
     1201 ccgccacctc ctggccgatc accacgtcct gccttccact cccggcaatc ttcatgagcc
     1261 ccatccgtcc caccacgatg tcctcgggcg cgtagcggtg aggcgtgaac tccccgttgc
     1321 ccaggttctc cagccaccac ggcccggcga tgatgtcggc tcttccgctg ccggccacct
     1381 caccggcgag catctcctcg ttgtactgga tcggggcgag cgtccgtacc ggccagccgc
     1441 ccgccgtggg atccttcggc acctcgaaga gctgggggta atggcgggga ttgttgtacg
     1501 cgctgtggta ggagaccgcg agggccagcc ggccgccccg gccgaacggg ccgaggagcg
     1561 agccgtgcgg ccagtcgccc attcccgttc cgatctcgtg cagcttccac gtgccggcaa
     1621 tcgggtccac ggctttcagc cagcacagct gggcgctcag atcggagtag aagtcgcctt
     1681 tggacgcgcg cggggcctgg atgcagatga gctccggtcg gccatcgcca tcgatatcat
     1741 gggtgttgat gacctgtgcc actcctggga tctctcgtct ctcccaggct ccccgccccg
     1801 ggatcccggg gttgcggtac caccacttcc cacagatgac gtccacgcgg ccgtccccgt
     1861 ccacgtcgca cggcaggatc tctacgccgg tggccggctt gtcccggtcc accatgcggt
     1921 ggcggagcct ggccaccggg ctcggggtgc tgacgcgctt ccacacctgc accaccgggc
     1981 tgcagtagta gaaccccgcg ctcttgccgg ccacctccag ctccccgtca ccgtccacgt
     2041 ccgcccacag cgcctggtgg gtcccctctc ccttcccgat caggtcctgg gtccagctcg
     2101 cgccgcggtc cgtggtgcga agctccagca cgcgcgcccc atggttgtag ggcgcatccc
     2161 acccgccctg ctccatctcg ccaatcatga cgcggaccga gccggcggct tcgcgcacgt
     2221 ccagcgagtg gccgtagacc acgtcatcca ggagtcgctg ttccttccag ggaccctcga
     2281 gcccgggatc cttgaggctg gatccagggt tggcaaacct ggagagccgg ccatccttga
     2341 actcggattc gaggatcagg atatcgggct tgccggtccc gcccaggtcc ac
//
LOCUS       DASRZS010000377         7990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5595872, whole genome shotgun sequence.
ACCESSION   DASRZS010000377 DASRZS010000000
VERSION     DASRZS010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5595872"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..717
                     /gene="pth"
                     /locus_tag="VFH83_09160"
     CDS             100..717
                     /gene="pth"
                     /locus_tag="VFH83_09160"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET6486574.1"
                     /translation="MFGLGNPGARYEETRHNVGFMVVDELARRLGSSFRKKLFRSYSL
                     TKATHQGKTLTLVKPLSFMNESGAAVREALRETGGEPSDILVVCDSLDLSPGNCRLKL
                     RGSSGGQKGLASIIRSLGTEDFPRIVIGIGRPAHKSQVVSHVLRAPRGADAERIEDGV
                     QRAADAVLRLMTEDPTRVMNDLNRKEPAADAGASGSAAHGSGAPG"
     gene            745..2163
                     /gene="tilS"
                     /locus_tag="VFH83_09165"
     CDS             745..2163
                     /gene="tilS"
                     /locus_tag="VFH83_09165"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET6486575.1"
                     /translation="MIDLDGRVRRALRQAGVPKNAHVLVGISGGPDSTALLLSLSRLR
                     RDQPLRLTACIVDHGIRDAAAVEGDIRSVRLLCGSLGVALREAAIPAGECRKRARELH
                     RSLEEVARDGRHRLLREAAAAAGAGFVALGHTQDDLIETLLMRVLQGAGERGLAGMPA
                     RRGSLLRPLLSCTRRDIVAYLASLGQSWRVDETNEDPSYLRNRIRQSLIPVLDASFEG
                     YRRGLLSMARRLTAVQDLVDTQAAAIRWRPFGTVAGAAFAVARESFLAAPPAARAASL
                     LALYDRIRSSSAPRRLPWRFVAPALGPRAAVGSGWILRGHGVALRVRGQEVVWGPDIA
                     SGCKKGYFMEVSETESRTIREFGLRVSLIRSDGKSQDLARGFAILPRSVSPPLVLRSR
                     RKGDQIILDGGRKTVKALLAGWKVSSEDRDTIPVLVDRQGVVAVLGGAVGYESRARAG
                     VLAGNEVDAERILVHICRDREE"
     gene            2165..4078
                     /gene="ftsH"
                     /locus_tag="VFH83_09170"
     CDS             2165..4078
                     /gene="ftsH"
                     /locus_tag="VFH83_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET6486576.1"
                     /translation="MSNNNDNRDDKGPDPRVPRFNFNNRIALISLLILVSFFVFFFVY
                     NDRTMSQELSYSTFQSYLDEGRVDTVKIIDQYEIQGSLKTSSGEVSLFKTTIPYPDPD
                     LMKTLKDKGVKLSGGAKAVSPLAVLIQFLPWVIGFFFIWFMFRQVQGNGNRAFSFGKS
                     RAKRYLDSGKRITFTDVAGQEEAKYELQEVVEFLKSPQKFARLGAKIPKGVLLVGMPG
                     TGKTLLAKAIAGEANVAFFHMSGSDFVEMFVGVGASRVRDLFEQGRRHAPCILFIDEL
                     DAVGRTRGAGYGGGHDEREQTLNQMLVEMDGFDTKDGVIILAATNRPDVLDPALLRPG
                     RFDRQVVVDMPDIKERELILKLHARRLPLSENVDLSRLARATPGSSGADLANLVNEAA
                     LFAGRKGKDRVEMDDFEEARDKTLMGVARKSKVIPPKEKLMVACHEAGHGLLHYYLKN
                     ADPLHKITIIPHGRALGVAFSLPEDDAYSRNKGWLHDRITIAMGGYAAERIIYEETTT
                     GAANDLEQATEIARKMVCEWGMSELLGPVAYGQKEEPIFLGKEIARHKDYSEETARAI
                     DTEIKKIVEASLSQALSLLTEHKDQLKRLSDTLIIRENLDDDEIRVLLGFPPRKREPD
                     PAVNGVVAAGNGH"
     gene            4115..6025
                     /locus_tag="VFH83_09175"
     CDS             4115..6025
                     /locus_tag="VFH83_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486577.1"
                     /translation="MSAHRVPPLFLSLLLLVLTGAVPLSAQSADAGTGPAPQAEAFLA
                     KARQAIAAGSAAQAASLIASALQLFPGYSEALYLKGRLESADRSSTMLALADLDQAVG
                     SSTWTVTDPDEARQELAGLLLRTGQVQRAVALLRPLVEAHPENEAAAALLARAYARAG
                     DDRAEQAVLSQAQALFPLAEDLTILQAGLQERQGRRAAARSSIAAALRARPDNLPLLL
                     AAARLAPDAKDRLTAVEAYAQKGGKDPLAAVIALESSPPSSTKYVTLFLDDGGLGHQD
                     LTTRVAAAVGRSGAAASALQNALGGFSGNRDLDQDGDGFWEERWTFSNGSPVEWIRDT
                     AEDGVPDYTAEFTGGRVASLSYRPEAGTTTSVQYSRYPFVETAREAGPAGITVYYLKP
                     YTLQAAFLQTIPTSPIKGVAPVISARIRLPSVAQILAAAYRSEDLGADGATRIRATSI
                     DHGRRVYLEEDTNGDGLMDHRVWFQDGQPVRGERSLDGGVTFPIGETWRAGILVSEAI
                     DTNGDGKIDFRQSFAPRPSKSWDYNEDGMDDSRETPGPEGTDVLEFSTALNGVFDVRL
                     VFLGPKLVRASISGATIGITEDPARGVTWIGPPAPASVSVEGLDDGIHVLSDTRFLSF
                     SYGGIHYMEEIR"
     gene            6022..7821
                     /locus_tag="VFH83_09180"
     CDS             6022..7821
                     /locus_tag="VFH83_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6486578.1"
                     /translation="MKTRPVMIILAVALVAGCASTPNRGIRGSHAIPPLPAVQLKEIR
                     DLLKDGSFLEALQGVSALRRDAGTLTASDLDPLEAEASTALSAEFKSSLAAKHFDEAY
                     RLLDSAMAMGRADLVAPWTGPSLLQEVASSQEASGDLVPALLTRLRTLKLSGESPPSD
                     LEAALLDAQKLSNRAAVRALIPMLKARGLDVPTDADVLANAPASFPQMLTGTVTILVN
                     RGIKVQNGVGYPDQVIGSGFFIDPRGYLLTNHHVIESEVDPKYEGYSRLYVRLSDSPA
                     GERIPAKVVGYDSIFDLALIKAEVTPQYTFGGYPDPSLAPGDHIYAIGSPAGLEKTIT
                     SGIISALGRRFLQVGDTMQVDVPLNPGNSGGPLLSDKGDLLGVVFAGLQQFEGINFAI
                     PYTWVERALPALYRGGEAVHPWMGMALAETDEGLQVIYVMPDEPGQKAGMQVGDTLLA
                     IDGQAYTKIADVQQALLDYDPPSLVRVTYSRGGVKGEALICLSKRPDQPVETALERDA
                     RDPVIYPLFGMQLENVGTFLWRGNYIVKSVIKGSIADESGISAEDPLTLQDWQVDKDK
                     GYAAMWIVIKKRKAGFIESPVEIAAYLETNNFV"
BASE COUNT         1374 a         2583 c         2681 g         1352 t
ORIGIN      
        1 gctgccgcgg aaggcgccga gaagaccgag gaaaccgagg gagccgcgga agccgaggag
       61 accaagggcc ggaagaagga gtagctcctg tcgcttgtcg tgttcgggct gggcaacccc
      121 ggtgcccgat acgaggaaac ccggcacaac gtggggttca tggtggtgga cgagcttgcg
      181 cggaggctcg gatcgagctt ccgcaaaaag ctcttccggt cctacagcct gaccaaggcg
      241 acgcatcagg gcaaaaccct cacgctggtg aagccgcttt cctttatgaa cgagtccgga
      301 gcggccgtcc gagaggcgct ccgggaaacc ggcggcgaac cttcggatat cctcgtggtg
      361 tgtgattccc tcgacctgtc tcccggcaac tgccgcctga agctgcgagg ttcctcgggc
      421 gggcagaagg ggctcgcgtc gatcatccgg tccctgggca ccgaggactt cccccgaatc
      481 gtcattggga tcggcagacc ggcgcacaaa agccaggtgg tgtctcacgt gctgcgcgcc
      541 ccgcgcggcg cggatgccga gcgcatcgag gacggcgtcc agcgcgccgc ggatgccgtg
      601 ctgcgcctga tgacggagga cccaacccgg gtcatgaacg atctcaaccg caaggagccg
      661 gccgctgacg cgggagcttc cggctcagcc gcccacggct cgggcgcccc cggataggcg
      721 ctccggctga ggcgcccacg tgccatgatc gatctggacg gccgcgtccg ccgcgctctt
      781 cggcaggcgg gggtaccgaa gaatgcccat gtcctggtgg ggatctccgg cggtccggat
      841 tccaccgcgc tcctcctctc cctttcgcgg cttcgcaggg atcaaccgct tcggctcacg
      901 gcgtgcatcg tcgatcatgg catccgagac gcggcggccg ttgaagggga catccgttcc
      961 gttcggctgc tgtgcggctc cctcggagtc gcgctgaggg aagccgccat cccagccggg
     1021 gagtgccgca aacgggcgcg cgagctccat cggagcctgg aggaggtcgc gcgggacggc
     1081 cgccatcgcc tccttcgaga agccgcggcc gcggcgggcg ccggtttcgt ggcgcttggc
     1141 catacccagg acgatctgat cgagaccctc ctgatgcgcg tgctccaggg tgccggagag
     1201 cgcggccttg ccggcatgcc cgcgcgccgc ggaagccttc tgcgaccgct cctctcctgc
     1261 acgcggcggg atatcgtcgc gtaccttgca tcgctgggcc agagttggcg cgtggacgag
     1321 acgaacgagg acccgtccta tctgcgcaac cggatccgac agtccctgat tccggtgctc
     1381 gacgcgtcct ttgaagggta ccgacgcggg ctcttgtcca tggcccgcag gctcaccgcg
     1441 gttcaggacc ttgtggatac tcaggccgct gccatccggt ggcgcccctt cgggacggta
     1501 gcgggcgcgg cgttcgccgt ggcccgtgag tcgttcctgg cggcgccgcc ggcggcgcgc
     1561 gccgcgtctc tcctggctct ctacgatcgg atccgttcct ccagcgctcc ccgacgtctg
     1621 ccctggcgat tcgtagctcc agccctgggg ccgcgggccg cggtggggag cggctggatc
     1681 ctccggggcc acggcgtggc tctgcgcgtc cgcggccagg aggtggtgtg gggacccgat
     1741 attgccagcg ggtgcaaaaa ggggtacttt atggaagtat ccgagaccga gagcaggaca
     1801 attcgtgagt tcggactgcg agtgagcctc atccggagcg acgggaagtc tcaggatctg
     1861 gcgcggggat tcgcgattct cccaaggtcc gtctcccctc cgctcgtgct caggtcaaga
     1921 cgcaagggag accagatcat cctcgacggc ggccgcaaga ccgtgaaggc actgctggcc
     1981 ggatggaagg tgagctcgga ggatcgggac accattccgg tcctggtgga ccgtcaaggg
     2041 gtggtcgccg ttctaggtgg cgccgttggc tatgagagcc gggcgcgtgc gggcgtcctc
     2101 gccggcaatg aggtggacgc cgaacgcatt ctcgtgcaca tttgcaggga cagggaagag
     2161 tgacgtgagc aacaacaacg acaaccgtga cgacaaaggc ccagaccccc gggttccccg
     2221 gttcaacttc aacaaccgca tcgccctgat ctccctgttg atcctggtgt ccttcttcgt
     2281 cttcttcttc gtctacaacg atcggacgat gtcccaggag ctctcctatt ccaccttcca
     2341 gtcttatctc gacgagggcc gggtggacac ggtgaagatc atcgaccagt acgagatcca
     2401 gggatccctc aagacttcct cgggagaggt gagtctcttc aagaccacga ttccctatcc
     2461 ggacccggac ctcatgaaga ccctcaagga caagggcgtg aagctctccg ggggcgccaa
     2521 ggccgtttct cccctggccg tgttgatcca gttcctgccg tgggtcatcg gcttcttctt
     2581 catctggttc atgttccgcc aggtccaggg gaacggaaac cgggcgttct ccttcggcaa
     2641 gagccgggcc aaacgctacc tggattccgg aaagcggatc accttcacgg acgtggcggg
     2701 tcaggaggag gccaagtacg agctccagga ggtggtggag ttcctgaaga gtccgcagaa
     2761 gttcgcgcgc ctgggtgcaa agatccccaa gggcgtgctg ctggtaggga tgcccggtac
     2821 cggcaagacg ctcctggcca aggccatcgc cggtgaggcc aacgtcgcgt tcttccacat
     2881 gtccggatcc gacttcgtgg agatgttcgt aggcgtgggg gccagccggg tccgggacct
     2941 cttcgagcag ggccgccgcc acgctccgtg catcctgttc atcgatgagc tggacgcggt
     3001 cgggcgcacg cgcggcgcgg gctacggggg cggccacgac gagcgcgagc agacgttgaa
     3061 ccagatgctg gtggagatgg acgggttcga caccaaggat ggcgtcatca tcctggcggc
     3121 gaccaaccgt ccggacgtcc tggaccccgc gcttctcagg cccgggagat tcgaccgcca
     3181 ggtcgtcgtg gacatgccgg atatcaagga gcgtgagctg atcctcaagc tgcatgcccg
     3241 ccgtcttccg ctctcggaga acgttgacct gtcccgcctg gcgcgcgcca cgcccggatc
     3301 ctcgggcgcg gatctggcga accttgtcaa cgaggcggcg ctcttcgcgg gccgcaaggg
     3361 caaagaccgc gtcgagatgg acgacttcga ggaggcgcgg gacaagacgc tgatgggggt
     3421 ggcccgcaag agcaaagtca tcccgcccaa ggagaagctg atggtggcct gccacgaggc
     3481 cggccatggt ctgctccact attacctcaa gaacgcggat ccgttgcaca agatcacgat
     3541 cattccccac ggccgggccc tgggagtggc cttctccctg ccggaagatg acgcttactc
     3601 caggaacaag ggctggcttc acgaccggat caccatcgcc atgggcggct acgcggcgga
     3661 gcggatcatc tacgaagaga ccacgaccgg tgccgcgaac gacctggagc aggccacgga
     3721 gatcgctcgc aagatggtgt gcgagtgggg gatgagcgag cttctgggcc cggttgccta
     3781 tggccagaaa gaggagccga ttttcctggg caaggagatc gcccgccaca aggactactc
     3841 ggaggagacg gcccgcgcca tcgacaccga gatcaagaag atcgtggagg catcgctcag
     3901 ccaggcgctc tctcttctga ccgagcacaa ggaccagctc aagcgcctct cggacacgct
     3961 catcatccgc gagaacctgg atgacgacga gatccgggtg ctcctggggt tcccgccccg
     4021 caagcgggag ccggatcctg ccgtgaatgg ggtggttgcc gcgggcaacg gccactaagg
     4081 ggctgttcgc tcgcctgccg atacctaggg aagaatgtcg gcacatcgag tcccaccgtt
     4141 gttcctgtcg ctcctcctgc tggtccttac tggagcggtc ccgctgtcgg cccagagtgc
     4201 cgacgccggt accggacctg cgccccaagc cgaggcgttc ctggccaagg cgcggcaggc
     4261 catcgccgcc ggctccgccg cacaggccgc gtctctgatc gcctcagccc tccagctctt
     4321 tcccggctat tccgaggctc tttacctcaa gggccgcctg gagtcggccg accgatcctc
     4381 gaccatgctg gcgctcgccg acctggacca ggcggtgggc tccagcacgt ggaccgtcac
     4441 ggaccccgac gaagctcgtc aggagctcgc cgggctgctc cttcgaacgg gacaggtgca
     4501 gcgcgcggtc gcgctcctgc ggccgctggt cgaagcgcac ccggagaacg aggccgccgc
     4561 ggcgctcctg gcccgggcgt acgccagggc cggtgacgat cgtgccgaac aggcggtcct
     4621 gtcccaggcg caggctctct tccccctggc ggaggatctc acgatcctcc aggcgggact
     4681 gcaggagcgg caggggcgga gggcagccgc gcgctcgtcg atcgcggccg cgctgcgcgc
     4741 gagacccgac aaccttccgc tgctcctggc tgccgcgcgg ctcgccccgg acgcgaagga
     4801 ccggctgacc gccgttgaag cctatgcgca gaagggggga aaggaccctc tggctgcggt
     4861 catcgcgctc gagtcttcgc cgccgagctc aacgaagtac gtgaccctct tcctggatga
     4921 tggcggtctc ggtcaccagg acctcaccac gcgggtcgcc gcggctgtgg gcagatccgg
     4981 cgcggcggcc tcggcgctcc agaacgctct tggcggcttc tcggggaacc gcgacctgga
     5041 tcaggacgga gacggattct gggaagagcg gtggacgttc agcaacgggt caccggtgga
     5101 atggatacga gacaccgcgg aggatggggt accggactat acggccgagt tcacgggtgg
     5161 gcgagtcgcc tcgctgagct atcgccctga ggccggcacg accacatcgg tccaatacag
     5221 ccggtatccg tttgtcgaaa cggctcgaga agcgggaccc gcggggatca cggtctacta
     5281 cctgaagcca tacacgcttc aggccgcatt cctccagacc attccgacca gtcccatcaa
     5341 gggcgtggcg ccggtcattt ccgccaggat ccgtctccct tcggtggccc agatcctggc
     5401 ggccgcttac cgaagtgagg accttggcgc ggatggggct acccggatcc gcgcgacctc
     5461 tatcgaccac ggacgcagag tctacctgga agaggacacg aacggagatg ggctgatgga
     5521 ccaccgggtc tggttccagg acggacaacc ggttcggggc gagaggtccc tggatggggg
     5581 agtcacgttc cccatagggg agacgtggcg ggcaggtatc ctggtgagcg aggcgatcga
     5641 cacgaacggg gatggcaaga tcgatttccg ccagagcttc gcgccgcggc catcgaagtc
     5701 ctgggactac aacgaggatg gcatggatga tagtcgggag actccgggtc ctgaagggac
     5761 ggacgtgctg gagttctcta cggcgctcaa cggagtattc gacgtgcgcc tcgtgttcct
     5821 tggcccgaag ctggtgcgag ccagcatctc gggagcgacg atcggcatca cggaggatcc
     5881 cgcacggggg gtcacgtgga tcgggccgcc ggcaccggcc tccgtgtccg tcgaaggcct
     5941 cgatgacggc attcacgtgc tcagcgacac ccgcttcctt agtttttcct acggcggtat
     6001 tcattatatg gaggagattc ggtgaagacg cgacccgtga tgatcattct tgccgtggcc
     6061 cttgtggccg ggtgcgcctc gacgccgaac cgcgggatcc gcgggagcca cgccattccc
     6121 ccgctccccg cggtgcagct caaggagatt cgggatctgc tgaaggacgg ctcgttcctg
     6181 gaggcgctcc agggtgtgtc cgcgctgcgc agggatgcgg ggacgttgac cgcgtccgac
     6241 ctggacccgc tggaggcgga ggcgtccacc gccctttccg ccgagttcaa gagctccctt
     6301 gccgcgaagc acttcgacga ggcctatcgc ctcctggact ccgccatggc catgggaagg
     6361 gcggacctgg ttgccccctg gaccggtcca tccctgctcc aggaggtagc ctcttctcag
     6421 gaagcatccg gtgacctggt cccggccctg ctcacgcgtc tgcggaccct gaagctctcg
     6481 ggggagtctc ctccgtccga cctggaggcc gcgcttctgg acgcgcagaa gctctccaac
     6541 cgggccgccg ttcgggctct gatcccgatg ctcaaagcga gggggctcga cgtcccgacg
     6601 gatgcggacg ttctggccaa cgcgcccgcc tcttttcccc agatgctgac cggaacggtt
     6661 accatcctgg tgaaccgggg catcaaggtg cagaacggcg tgggctatcc cgaccaggtc
     6721 attggcagcg gttttttcat cgaccctcga gggtacctct tgaccaacca ccacgtgatc
     6781 gagagcgagg tagaccccaa atacgagggg tactcccgat tgtacgtacg gctctcggat
     6841 agcccggcgg gagagcggat tcccgccaag gtcgtggggt acgactccat cttcgacctg
     6901 gccctgatca aggccgaggt gaccccgcag tacaccttcg gaggctatcc ggatccttcc
     6961 ctggcacccg gggatcacat ctacgcgatt ggatctccgg cgggtctgga gaaaaccatc
     7021 acctccggaa tcatctccgc cttgggaaga cggttcctcc aggtgggcga caccatgcag
     7081 gtcgacgtgc ccctcaatcc aggcaacagc ggcggccctc tactgagcga caagggagac
     7141 ctcctggggg tggtcttcgc gggactccag cagttcgagg gaatcaactt cgccatcccc
     7201 tacacctggg tcgagcgggc gctgccggcc ctgtatcgcg gcggggaggc ggtacacccg
     7261 tggatgggga tggcgctcgc cgagacggac gagggtctgc aggtgatcta cgtcatgccg
     7321 gatgagcccg gccagaaggc cggcatgcag gtgggcgaca ccctcctggc catcgacggg
     7381 caggcataca ccaagatcgc ggacgtccag caggcgctgc tggactacga tcctccctcg
     7441 ctggtccgcg tgacctacag ccgcgggggc gtgaaggggg aggcgcttat ctgcctctcc
     7501 aagcgcccgg accagccggt ggagaccgcg ctggagcggg atgcccggga tccggtcatc
     7561 tatcccctct tcgggatgca actggagaac gtcggcacgt ttctctggcg cgggaactac
     7621 atcgtcaaga gcgtgatcaa gggttccatc gcggatgagt ccggcatctc ggccgaggat
     7681 ccactcaccc tccaggactg gcaggtggac aaggacaagg gctacgccgc catgtggatc
     7741 gtgatcaaga agcgcaaagc cggcttcatc gagagtccgg tggagatcgc tgcctacctg
     7801 gagaccaaca acttcgtcta gccgctgatg gagcctccga caggctccta agcgtagggc
     7861 cgaggtctct ccgcgcgcgg atcccggggt gacgcgggcc tcttgcgcag cgtgacctgg
     7921 ttgccgacct cattgtagag gacctcgccg aagtagatct tggtgagaaa gatcccccgg
     7981 ccgttgaacg
//
LOCUS       DASRZS010000378         7374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2129252, whole genome shotgun sequence.
ACCESSION   DASRZS010000378 DASRZS010000000
VERSION     DASRZS010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129252"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFH83_09185"
     CDS             <1..363
                     /locus_tag="VFH83_09185"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6486579.1"
                     /translation="VPATVENGCMAFIPGTHTLGTVRHEDRQYYLEIAREELEPRLPQ
                     AVPIECNPGDVVLFHNLLFHCGLPNHSRTIRWSADWRYQDAREPTHRPKNGHLARSAS
                     RPNEVVKNPSDWAHRTFV"
     gene            complement(428..2569)
                     /locus_tag="VFH83_09190"
     CDS             complement(428..2569)
                     /locus_tag="VFH83_09190"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET6486580.1"
                     /translation="MPDEDARWSDVFFQEEPEPTVSYRSRLAVYEESLTRGRFVGRGW
                     NAAGFISFYDGRIDPLLDTTPQAFWLEMDGHLLGSHWVWDGVERPPIPEGQAVAVALR
                     HTVRPVAVRVHTRLDGTAVLTRWLEVTNAGSEPAALSAAFPWSGLLQRTHRWRSHLGG
                     TGRPLYSIGYFEHARWGTEGAFRWFDLPAAGYRIDGRYHRERHRHPLVVIRNNATGEH
                     FILQIAWSGGYLVELNLNPEVAGPGAAEPAAILSFRAGPDAPAPLRVIDPGETVLTPE
                     VHLGLVMGDLDSAIQEMHEHLRTSVFLPQPRGHGGWLESGIGPEIEITTEQVHHAIDT
                     AADLGAEVFFIDASWYSRPRANWFSTVGDWEVQRARFPGGLEPFRRRVHEKGMLWGLW
                     MEPERLGTESRAFREHQDWLVHAYDGEQRPEGLIDLGIPEAARWVEEQIVKVIDSHQC
                     DFFRLDYNTQPGVSTVRQRGAFLENATWRYYESLYAIFDRLRTRYPQVIFENCAGGGG
                     RTDIGMVRRFSHTWVTDWQIAPRSFAITNGMTMALPPEHVDRLLGGQSGHITAELDFQ
                     SRLLLFVRPTFGFLHPRGAVPNPIMGERTREWIRLYREFVRPYLSPGRIYHHTPEVDG
                     LEPQGWGVLELCSRDRSRGICGLFQLSAPTEPDYVLRLRGLDAGRRYRVRWGNSGRTS
                     VLDGFVLMQQGLTIRLEGALTSELLVFEPVE"
     gene            2619..3431
                     /locus_tag="VFH83_09195"
     CDS             2619..3431
                     /locus_tag="VFH83_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6486581.1"
                     /translation="MNTKLTKEQIDTYQRDGALVYRSFLSEAECDQMLKDIEEAIGRM
                     GQDVVADLKEYGEVAKPNTDDFYSRVFIQRVNMWKVSDAIKRVFLGPELGEMVSRLAG
                     VDGMRVWHDQTLQKQPWANPTAWHLDNPYWSFHSHQAISIWIALDDATVENGCMCYLP
                     GTQKTAEFARNSTIGKDFGGLFKVYPEWKTLKPIVGTMKRGDCGFHNGLTAHGAGPNM
                     TPGYRRAMTCAYMPDGSTFNGNKNILPADYMGTLKIGDLLNNDALNPIVWKK"
     gene            complement(3474..4664)
                     /locus_tag="VFH83_09200"
     CDS             complement(3474..4664)
                     /locus_tag="VFH83_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018246006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET6486582.1"
                     /translation="MRITDLKCAVIGDNPVIRITTDEGIDGWGEAENSKPYLKPHVLF
                     YRQHVLGEDPTNVERVMLKIRRLGSFKPWGSAVSAIEWALWDIAGKAAGVPVYKLLGG
                     KVRDRVRVYNGGVRFPMTGSEPEDYAADVRRMAASPEGFSLIKAAVAFHSDMPRNVPD
                     FSYGEPHSGPAHPNRAPLTERGLNHVIECVEAMKDALGDRVSLALDCGPGWMLSDAIR
                     FARAVEPLNLAWLEDLLTGDYVPYVHADLYRDLTTSTSTPIHTGEQIYLRQNFKELIE
                     RQAVRIVGPDPCDVGGMAELKWVAEYADLHGILMAPHGVIDGAIGLAALVQVSATLPQ
                     NYIAFEYPVARPSWWYDIVTGLPNPIVRGGFIDVWDRPGLGVDLIPEAAASHLSEEDK
                     SFFD"
     gene            complement(4678..5406)
                     /locus_tag="VFH83_09205"
     CDS             complement(4678..5406)
                     /locus_tag="VFH83_09205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6486583.1"
                     /translation="MQQSLSGKVVIITGASSGIGAAAARELARLGCKLALASRSTDTL
                     TALAAELGPSACAVPTDVTSGAEVDRLVSTTLERFGGIDILLANAGIYVPGKLWEGDP
                     EKLARLVDVNVNGVLRCIRAVLPPMKARHAGDILVTSSISGHLAIPWEPVYSASKHAV
                     QALVHTLRRQAAPDGIRVGAVAPGMVANELWGITTAGEVERHVRERSALRNEDVVDAM
                     VFMLTRPPHVTVRDLVMLPQSQDL"
     gene            complement(5477..6139)
                     /locus_tag="VFH83_09210"
     CDS             complement(5477..6139)
                     /locus_tag="VFH83_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486584.1"
                     /translation="MSLAILYADELSGFYRSRVMLVLWVGMPVMALVLHAVNPDLRGQ
                     MTLAQFSMLLIATIASTISAVMLSVGIITEKTRGVYALFLVRPVRRWCILVAKFLAVF
                     TCVAVASVLTLLVGALFDWLRGAAPDARTLAEAAQAAATSFASIVIASSAGILIGVLS
                     PSVLVGAILVIYGANQLSAAGYLPLVLKLQPAWAYSLGIGCAVSLVLLAVSIVSFQRK
                     QI"
     gene            complement(6136..7125)
                     /locus_tag="VFH83_09215"
     CDS             complement(6136..7125)
                     /locus_tag="VFH83_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486585.1"
                     /translation="MPDRTAIAVFRNVAKSYGSVAALRGLSFEVLRGEIFGFIGPNGA
                     GKTTTMKTLVGLLTDFTGTLTVDGLPMPEHRAALYHSVGYMPQAIAFQEWRTVDQALS
                     TFGRLCGLGRPVLAERIPRILDRVGLQGVQDRKVMHLSGGMVQKLGLAQALLHEPRLL
                     VLDEPVAGLDPASRIQVKAILASLRDAGVTILFSSHILSDVQDVADRIGIIRSGTMLT
                     VGTLQDLKSRFAVNDDIEIVLSRDAETWRELRSVAGVLDVEPSGRGSLLLHLLPGADV
                     DATAHEVLTRLLGSGNRVRSFHPLAPSLDQLYLKYVGAAAGGADAPPQAGTSR"
     gene            complement(7135..>7374)
                     /locus_tag="VFH83_09220"
     CDS             complement(7135..>7374)
                     /locus_tag="VFH83_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486586.1"
                     /translation="QPRGGEASAASPRTEVQSESLKALEGGARLVPLMDAELTDQAAY
                     DLRAAFIAWPPTGFGSKDAITALAARLAAVVKSSK"
BASE COUNT         1297 a         2504 c         2425 g         1148 t
ORIGIN      
        1 gtgccggcca cggtggagaa cggctgcatg gctttcattc ctggcaccca cacgctggga
       61 acggtccgac acgaggatcg gcagtactac ctggagatcg cgcgagagga gctggagccc
      121 cgcctgcccc aggccgtccc catcgagtgc aaccctggag acgtagtcct cttccacaac
      181 ctgctgttcc actgcggact gcccaaccac tccaggacca tccgctggag cgcggactgg
      241 cgctaccagg acgcccggga gccgacccat cgacccaaga acggtcacct ggcccgctcc
      301 gcgtcccgac cgaacgaggt agtgaagaac ccttccgact gggcccaccg gacgtttgtc
      361 tgagcgaccg agacgcgtcc cgacccgggg gccctcatcc gctccagccc ctctctgggc
      421 ccgcccccta ctccaccggc tcgaagacca ggagctccga ggtgagggcg ccttccagcc
      481 ggatcgtcaa gccttgctgc atcaaaacga agccgtccag gacgctcgtc ctgcctgagt
      541 ttccccatcg cacccggtag cggcgtcccg catccaagcc gcgaagtctc agcacgtagt
      601 ccggctccgt gggggccgat agctggaaca ggccgcagat cccgcggctc cggtcacggg
      661 agcacaactc caggacgccc cagccctggg gctccagacc atccacttcc ggagtgtgat
      721 ggtagatgcg gcccggcgac aggtacgggc ggacgaactc ccggtacagc cgaatccact
      781 cccgggtccg ctcccccatg atcgggttgg gcacggcacc ccggggatgc aggaacccga
      841 aggtggggcg aacgaagagc aggagccggg actggaagtc cagctcggcc gtgatgtgtc
      901 cgctctggcc gcccaggagg cgatccacgt gctccggcgg cagggccatg gtcatcccgt
      961 tggtgatggc gaaggatcgc ggcgcgatct gccagtcggt cacccaggtg tggctgaacc
     1021 ggcgcaccat gccgatatcg gtcctgccgc cgccccccgc gcagttctcg aagatcacct
     1081 ggggataccg ggtgcgaagc cgatcgaaga tggcatacag cgattcgtag tatctccagg
     1141 tggcgttctc caggaacgcg cccctctgac gcaccgtgga gaccccgggc tgggtgttgt
     1201 agtcgagccg gaagaagtcg cactggtggc tgtcgatgac cttgacgatt tgctcctcga
     1261 cccagcgcgc agcctccggg atccccaggt cgatgagacc ttcagggcgc tgctccccgt
     1321 cgtaggcatg gaccagccag tcctggtgct cccgaaaggc ccggctctcg gtccccagcc
     1381 gctcgggctc catccacaga ccccagagca tccccttctc gtggacgcgc cgacggaacg
     1441 gctcgagccc gcccgggaag cgcgcgcgct gaacctccca gtctcccacc gtggagaacc
     1501 agttggcccg cggcctggag taccagctcg cgtcgatgaa gaacacctcc gctcccaggt
     1561 ccgccgcggt atcgatcgcg tggtgaacct gctcggtcgt gatctcgatc tccggtccga
     1621 tgccggactc cagccagccc ccatgcccgc gaggctgcgg gagaaagacg ctggtgcgaa
     1681 ggtgctcgtg catctcctgg atcgcggagt ccagatcccc catcacgagg ccgaggtgga
     1741 cctccggcgt gagcacggtc tcgcccgggt cgatgacgcg aagaggggcc ggtgcgtctg
     1801 gcccagctcg gaaggagagg atcgccgcgg gctccgcggc gcccggcccg gcaacctcgg
     1861 gattcaggtt gagctcgaca aggtagcccc cggaccaggc gatctgcagg atgaagtgct
     1921 caccggtggc gttgttgcgg atcaccacga ggggatggcg gtggcgctcg cggtggtagc
     1981 ggccatcgat ccgatatccg gccgctggca gatcgaacca gcggaaggct ccctcggttc
     2041 cccaccgggc gtgctcgaaa tagccgatgg agtacagggg cctgccggtc cctcccaggt
     2101 gcgacctcca ccggtgggtc ctctgaagca agccactcca cggaaacgcc gcggaaaggg
     2161 ccgcgggctc cgacccggcg ttcgtgacct ccagccaccg tgtcaggacg gccgtcccgt
     2221 ccagccgcgt gtggaccctg accgccacgg gacgcacggt atgccgcaag gctacggcga
     2281 ccgcttgtcc ctccgggatt ggcggtcgct ccacgccgtc ccacacccag tgagagccga
     2341 ggaggtgtcc gtccatctcc agccagaagg cctggggagt cgtgtccagg agagggtcga
     2401 tccggccgtc gtagaagctg atgaatccgg cagcgttcca ccctctgccg acgaaccgcc
     2461 cgcgggtgag cgactcctcg tacaccgcca ggcggcttcg gtagctcacg gtaggctcgg
     2521 gctcctcctg gaagaagaca tcggaccaac gggcatcctc gtcaggcatg gcctctctcc
     2581 tctcggtctc agtatcttcg aaatccagag gaaggaaaat gaacacaaag ctgaccaagg
     2641 aacagatcga cacataccag agagacgggg cgctggtcta ccggagcttc ttgagcgagg
     2701 ccgagtgcga ccagatgctc aaggacatcg aggaggccat cggccgcatg ggccaggacg
     2761 tggtggcgga cctcaaggag tacggagagg tcgccaagcc gaacacggac gacttctact
     2821 cccgggtctt catccagcgc gtcaacatgt ggaaggtgag tgacgccatc aagcgggttt
     2881 tcctgggacc cgagctgggg gagatggtga gccgtctggc cggtgtggat ggcatgcggg
     2941 tctggcacga tcagaccctg cagaagcagc cctgggccaa ccccaccgcc tggcacctcg
     3001 acaatccgta ctggtcgttc cacagtcacc aggccatctc catctggatc gccctggacg
     3061 acgccaccgt ggagaacggc tgcatgtgct acctgcccgg tacccagaag accgcggagt
     3121 tcgcgaggaa ctccaccatc ggcaaggact tcggcgggct gttcaaggtc tatccggagt
     3181 ggaagaccct gaagccgatc gtcgggacca tgaagcgggg ggactgcggg ttccacaacg
     3241 gtctcaccgc gcacggggcc ggacccaaca tgactccggg gtaccgcagg gccatgacgt
     3301 gcgcgtacat gccggacgga tccaccttca acggcaacaa gaacatcctg ccggcggact
     3361 acatgggcac cctgaagatc ggagatctcc tgaacaacga cgccctcaat ccgatcgtct
     3421 ggaagaagta ggagcgaacg gggcccgggg ttgcgacccc ggccccgcga tcctcaatcg
     3481 aagaagctct tgtcttcctc cgagaggtgg gaggctgccg cctccgggat cagatccacg
     3541 cccagaccgg ggcggtccca cacgtcgatg aagccacccc ggacgatcgg gttgggaagc
     3601 cccgtcacga tgtcgtacca ccaggaaggc cgcgccacgg gatactcgaa ggcgatgtag
     3661 ttctgcggaa gcgttgcgga gacctggacc agggccgcca gcccgatggc tccgtcgatg
     3721 accccgtggg gcgccatgag gatgccatgg aggtccgcgt attccgccac ccacttcagc
     3781 tccgccattc ctccgacatc acacggatcg gggcccacga tccgcaccgc ctggcgctcg
     3841 atcagctcct tgaagttctg gcgcaggtag atctgctccc cggtgtggat gggggtcgag
     3901 gtgctggtgg tcaggtctcg atagagatcc gcgtggacgt aggggacgta gtccccggtg
     3961 agcaggtcct ccaaccatgc caggttcagc ggctccaccg cccgggcaaa ccggatggcg
     4021 tcgctcagca tccagcccgg gccacagtcc agcgccaggc tgacccgatc ccccaaggcg
     4081 tctttcatgg cttcgacgca ctcgatcacg tggttgaggc cgcgctccgt gagaggcgcc
     4141 cggttgggat gcgccggacc gctatgcggt tcgccatagg aaaagtcggg gacgttgcgg
     4201 ggcatgtcgc tgtggaaggc aacggccgcc ttgatcaggg agaagccctc ggggcttgcc
     4261 gccatccttc ggacgtccgc ggcatagtcc tcgggctcgg aaccggtcat ggggaagcgc
     4321 acgcctccgt tgtagaccct gacgcgatca cggacctttc cgcccaggag cttgtacacg
     4381 ggaacgcccg ccgctttgcc cgcaatgtcc cagagcgccc actcgatggc gctcaccgcg
     4441 cttccccagg gcttgaagct cccgagccgg cggatcttga gcatgacccg ctccacgttg
     4501 gtcgggtcct cacccaggac gtgctggcgg tagaagagga cgtgcggctt cagatagggc
     4561 ttcgagttct ccgcctcccc ccagccgtcg atgccctcat ccgtcgtgat ccggatcacg
     4621 gggttatccc cgatgaccgc gcatttgagg tcggtgatcc tcatgcgggt gcgctcccta
     4681 caggtcctgg ctctgcggca gcatcacgag gtctcgaacc gtcacgtgag gcgggcgggt
     4741 gagcatgaac accatggcgt ccaccacgtc ctcgtttcgg agcgccgagc gctcgcgcac
     4801 gtgtcgctcg acttccccgg ccgtggtgat accccacagc tcgttggcca ccatcccggg
     4861 cgcgaccgct cccacgcgga ttccatccgg tgctgcctgc cggcggagcg tgtgcacgag
     4921 cgcctggacc gcgtgcttgg aggcgctgta caccggctcc cacggtatcg caaggtgccc
     4981 cgagatggag ctggtgacca ggatatcacc ggcatgacgg gccttcatgg gcggcagcac
     5041 cgctcggatg catcgcagca ccccgttcac gttcacgtcc accagtcggg ccagtttctc
     5101 cggatcgccc tcccagagct tcccggggac atagatcccg gcgtttgcca ggaggatgtc
     5161 gatcccgccg aaccgctcca gggtggtcga gaccagacgg tcaacttccg ctcccgaggt
     5221 cacgtcggtg ggtacggcgc aggcgctcgg gccgagctca gcggccagag cggtcagggt
     5281 gtcggtcgat cgcgacgcca gggcgagctt gcagcctagc cgagcaagct cccgcgccgc
     5341 tgcggctccg atcccagagc ttgcgccggt gatgatgaca actttccccg agagcgactg
     5401 ctgcacgatt gcctcctgag cggtttcggg cgcgttgccg cggtgtcggg cggcccaccc
     5461 ctggtccgct ttcggactag atctgctttc gttggaagga tacgatggac accgccaaca
     5521 ggaccagcga gacggcacac ccgatcccaa gggaataggc ccaggcgggt tgcagcttca
     5581 gcaccagcgg gaggtagccg gccgccgaga gctggttggc tccgtagatg accaggatcg
     5641 cgccgaccag cacggaggga gacagcacgc cgatcaggat gccggcgctg ctggcaatga
     5701 cgatgctggc gaagctcgtg gcggccgcct gtgccgcctc cgccaacgtg cgcgcgtccg
     5761 gcgccgctcc ccgaagccag tcgaagagcg cccccaccag cagcgtcaga accgaggcca
     5821 ccgctacgca ggtgaagacg gccaggaact tggccaccag gatgcaccag cgccgcacgg
     5881 ggcgcacaag gaagagcgcg tataccccgc gcgtcttctc ggtgatgatg cccaccgaga
     5941 gcatgaccgc cgagatggtg ctggcgatcg tggcgatgag cagcatggag aactgggcca
     6001 gcgtcatctg gccccggagg tccgggttca cggcatgcag gaccagggcc attaccggca
     6061 tcccgaccca gagcaccagc atcaccctgg atcggtagaa gccggagagc tcgtccgcgt
     6121 agagaatggc gaggctcatc ggctcgttcc cgcctggggc ggcgcgtcgg ctccccccgc
     6181 ggcggccccc acgtacttca ggtagagctg gtccaggctg ggggccagcg ggtgaaagct
     6241 tctgacccgg ttgccgctcc ccaggagccg ggtgagcacc tcgtgggccg tcgcgtcgac
     6301 gtccgctccc ggcaggaggt gcagaaggag ggacccgcgc ccgctgggct ccacgtccag
     6361 gacgccggca acggagcgga gctcccgcca ggtctccgcg tcccgcgaca ggacgatctc
     6421 gatgtcgtcg ttcaccgcga accggctctt gaggtcctgg agcgtgccca ccgtcagcat
     6481 cgtccccgag cggatgatgc cgatacggtc cgccacgtcc tggacgtcgc tcaggatatg
     6541 ggaggagaag aggatggtga cgccggcgtc ccggagggag gcgaggatgg ccttcacttg
     6601 gatgcggctt gcggggtcca gaccagccac aggctcatcc agcacgagca gcctgggctc
     6661 gtgcagcagt gcctgggcca gaccgagctt ctgcaccatg ccgccggaca ggtgcatcac
     6721 cttccgatcc tgaacaccct ggagacctac ccggtccagg atccggggaa tccgttccgc
     6781 cagcaccggg cggccgaggc cgcagagccg gccgaaggtg ctgagagcct ggtccaccgt
     6841 gcgccactcc tggaacgcga ttgcctgcgg catgtagccc acggaatggt agagcgcggc
     6901 gcggtgctcc ggcatgggga gcccatccac ggtgagggtg ccggtgaagt cggtgagaag
     6961 cccgaccagg gtcttcatgg tcgtggtctt gccggcgccg ttgggcccga tgaacccgaa
     7021 gatctctccc cgcaggacct cgaaactcag gccccgaagt gccgctacgg aaccatagct
     7081 cttggcgaca ttccgaaaca cggcaatcgc ggttcgatcc ggcacccaga tctcctactt
     7141 cgatgacttg accacggccg ccagtcgcgc cgccagggcg gtgatggcgt ccttggagcc
     7201 gaacccggtc ggcggccagg cgatgaaggc ggcgcgcaga tcgtaggccg cctggtccgt
     7261 gagctcggca tccatcagcg ggacgaggcg ggcaccgccc tccagggcct tgagggactc
     7321 gctttgaacc tcggtccgtg gcgacgcggc cgaggcctcg ccgccccgcg gctg
//
LOCUS       DASRZS010000379         7547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_205382, whole genome shotgun sequence.
ACCESSION   DASRZS010000379 DASRZS010000000
VERSION     DASRZS010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7547
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205382"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VFH83_09225"
     CDS             <1..680
                     /locus_tag="VFH83_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HET6486587.1"
                     /translation="PGEAHRSQPGKERAYLLLRGACEVTFDGIPSVMERASVFDEPPW
                     CLSLPAETPADVLALDSGAELAVQATVNPRRFGPRLYTPADCRSEDRGKGTMRETSTR
                     IVRTILEDPGAAHSNLVLGEVITYPGKWSSYPPHHHPQPEIYHYRFLPEGGFGVAVVG
                     DAAYVVRHGDTLLIPPEASHPQVSAPGYAMYYLWVIRHLDGNRYSTPVFEPQHLWVTD
                     PAAAIWP"
     gene            693..2558
                     /gene="iolD"
                     /locus_tag="VFH83_09230"
     CDS             693..2558
                     /gene="iolD"
                     /locus_tag="VFH83_09230"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HET6486588.1"
                     /translation="MKRIRLTTAQALVRFLDNQYVQVDGAQSKFVRGVFGIFGHGNVV
                     GIGEALAAGGHGLTYFQGHNEQGMAHAAVGFAKQMRRRQIIAVTSSIGPGALNMVTAA
                     ATATVNRIPLLVLPGDTFACRQPDPVLQQLEQFNDLGITANDAFRPVSRAWDRISRPE
                     QLLSACINAMRVLTDPAETGAVTLALPQDVQGESYEFPVEFFDRRVHRIERRPPTEAE
                     VEEAARLVTSSRRPLIICGGGVRYSDAGAALASFCETLGIPFGETQAGKGEVPWDNPY
                     NLGGIGVTGTQAANALAKSADLVIALGTRLSDFTTSSKWLFQDPRSRLLGINTNSFDA
                     HKMNGASVIADARLALEALGAALKKSGYKSQWGTEIQSARKEWNAEVDKLYCAEDPAG
                     LSQIRCLGELNERLLPKDAIVVAASGSLPGDMQRLWRPRASDTYHMEYGFSCMGYEIG
                     AALGAKLACPEREVFALVGDGSYVMLHSEIIASLQERAKINVLVFDNGGFQVIDNLQV
                     AQGIERFGNEWRQRDHASGRLTGPFLPIDFAANGESYGARGFRARTVEEFRAAIKGAL
                     AEKRTTVIDAKVTAKSMTHGYGSWWRVGTAEVSQNPAVEKAAREIHEESAKARAY"
     gene            complement(2620..3015)
                     /locus_tag="VFH83_09235"
     CDS             complement(2620..3015)
                     /locus_tag="VFH83_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6486589.1"
                     /translation="MITHVANVTLFVKDQQKALAFYVDKLGFEKRTDQPMGPDAPHWI
                     EVAPKGSKTVIVLYKPTPKAPGSSTYRRALSSIGTFAPFVLEVKDMEATCKELKAKGV
                     KFADPPKKQPYGWWATIKDPDGNVIGMHG"
     gene            complement(3109..3549)
                     /locus_tag="VFH83_09240"
     CDS             complement(3109..3549)
                     /locus_tag="VFH83_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486590.1"
                     /translation="MKTLSKVLLIIGLVLGCLGGLSRIATVALGDTIMATLRGSGAQL
                     QGNYSVAQKGLEQATAKTFSNIASIAGNLLLVLVGGALGLLACAEDTDRKWKVVFSLV
                     ALAAGLGLLAMRSWVCAGAYVIGGCLSLLAVDRPAGPAKDSQKV"
     gene            complement(3688..5556)
                     /locus_tag="VFH83_09245"
     CDS             complement(3688..5556)
                     /locus_tag="VFH83_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HET6486591.1"
                     /translation="MDQKVLFLDAATGFYRIRRYPVGRFFGPVDLGLHISAHHQSLNI
                     GVGLLAGSIFPGSNRLFVTGFSPCWRGFFVSSMGGAGLVFDNLGLNTVTILGKSATPS
                     VLYLNRTRGEEVEVELEPVDANAVWAQGRGGVYSMMDHVYARFAGRYATDPRILATGP
                     AALRTDMGAIASVPINQGKLSEVDTWAGRGGMGSKMLQEHGIAAVIYGGTYVDDDFRD
                     RTVADGWFQTKYQKRLLAKDMEATTKYRFDPSFNTGGTFGVNYAGIKGRIIAFNYRTI
                     FDTEADRLALHDRLVVQHYLKQFNEETIAPKHQRTCGEPCSALCKKMRDQYKKDYEPY
                     QAMGPLCGVFDQRAAERLCHQADLLGFDAISVGGVLAWMMELLVEGLLTPADLGVSGV
                     PSWKADGFDPVADSARNAAIGLELLQSIIERRGKVDLADGARKWGRRLSRETGKRLLD
                     RFLYVAFGRQGWMVPNQYWTPGVLAPMGVMGKYYMYYGNAFAPPRTLGRMCAERMEKE
                     LAIDTAGFCRFHRGWAEELIPEIFGNLYGLKDELLAGIRVTASRINSRNASVYWESER
                     NVDFLHTYLRRIRDVEGETDPQLVDWLDRFDKDKNEAALDWWFEMRKGIDESLREF"
     gene            complement(5636..6340)
                     /locus_tag="VFH83_09250"
     CDS             complement(5636..6340)
                     /locus_tag="VFH83_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6486592.1"
                     /translation="MISSAYRWVIGFSWVVLICVTTIVCSIFAKAGAYERFFKAMVRG
                     LFRVLVIPVRVRGTGHLAPQRAGRLYLCNHVSLFDVPLLCGYLPGMVRGVEWGPHFRW
                     PLYGLMIRRIGNIPIDRDDAFASMRSLQKAKRRLQAGHSLVIMPEAHRTADGELRPFK
                     RLPFHIAREAGVEVVPVGISGLYGLHRKGSWVIRSSRLLVRVGAPIPAGTVKTSTAAE
                     LRDLTRQRILELVERP"
     gene            complement(6337..7227)
                     /locus_tag="VFH83_09255"
     CDS             complement(6337..7227)
                     /locus_tag="VFH83_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET6486593.1"
                     /translation="MRILLVYNPNAAHGRAGTALPLVREAFQQEKVDIDERLTGGPED
                     AMHITAEAPLERYQGVVAAGGDGTLFETVNGYYRNPSTTRPPLGVLPVGTGNAFARDL
                     DLDAARWREAVAVIAAGKTRRVDVGRYRSQGEVMYYLNILGLGFVADVTLAASRLKLL
                     GNVSYTIGVILRTVFLRPFRVHLEADGRVVDTESIFAEISNTRYTSNFLMAPAASIDD
                     GLLDVTLLSRLNRRRLLSCFPLIFTGEHVRLPEVITFKARKILITTETPKVLTPDGEL
                     LGKTPVSIECLARDIAVFCR"
     gene            complement(7224..>7547)
                     /locus_tag="VFH83_09260"
     CDS             complement(7224..>7547)
                     /locus_tag="VFH83_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HET6486594.1"
                     /translation="PAGLRELEKRCLENNARFKDWECTESLLKRTDKALYLHCLPADI
                     TGVSCAQGEVAADVFERFRSPLYRQAGWKPYIIAAMILTSRMADPQRTLSDLLDRGES
                     REYTA"
BASE COUNT         1284 a         2575 c         2455 g         1233 t
ORIGIN      
        1 ggcccggtga ggcgcaccgc agccagccgg gcaaggagcg ggcctacctg ctgcttcgcg
       61 gggcgtgcga ggtcaccttc gacgggattc cgagcgtgat ggagcgcgca tccgttttcg
      121 acgagccgcc ctggtgtctg agccttcccg cggaaacgcc ggcggacgtc ctggcgctgg
      181 actccggagc cgagcttgcg gtccaggcaa ccgtcaatcc tcgccggttc ggccccaggc
      241 tctacacgcc ggccgattgc cgaagcgagg accggggcaa gggaaccatg cgggagacca
      301 gcacgcgcat cgttcggacg atcctggagg acccgggtgc cgcgcactcc aaccttgtcc
      361 tgggggaagt gatcacctac cccggcaaat ggtccagcta cccgccacac catcacccgc
      421 agccggagat ctaccattat cggttcctcc cggagggcgg gttcggggtg gcggtggtcg
      481 gggatgcggc gtacgtggtc cgacacggtg acaccctcct gatcccgccg gaggcgtcgc
      541 atccccaggt gtcggctccc ggctacgcca tgtactacct gtgggtcatc cgacacctgg
      601 acgggaatcg atactccacg ccggtattcg agccccagca tctctgggtc accgacccgg
      661 ccgccgccat ctggccctga aggagtgttg ccatgaaacg gatccgcctc acgaccgccc
      721 aggccctcgt gcgcttcctc gacaaccagt acgtccaggt ggatggcgcg cagagcaagt
      781 tcgtccgcgg ggtgttcggg atcttcggcc acgggaacgt cgtggggatc ggggaggcgc
      841 tcgccgcggg cgggcacggc ctcacctact tccagggaca caacgagcag ggcatggcgc
      901 acgccgccgt ggggttcgcc aagcagatga ggcgccgcca gatcatcgcc gtcaccagct
      961 ccatcggccc gggcgcgctc aacatggtca ccgccgcggc cacggcgacc gtgaatcgca
     1021 tcccgctcct ggtcctgccg ggagacacct ttgcctgccg gcagccggat ccggtcctcc
     1081 agcagctcga gcagttcaac gacctgggga tcaccgccaa cgacgcgttc cgtccggtga
     1141 gccgggcctg ggatcggatc tcccggcccg agcagctcct gagcgcctgc atcaacgcca
     1201 tgcgcgtgct caccgatccc gcggagaccg gggcggtgac cctggcgctt ccccaggacg
     1261 tgcaggggga gagctacgag ttcccggtgg agttcttcga ccgtcgggtc caccggatcg
     1321 agcgccgccc ccccacggag gcggaggtgg aagaagccgc caggctggtg acctcctccc
     1381 gccggccctt gatcatctgc ggcggcgggg tccgctactc cgatgcggga gcggcgctgg
     1441 cgtcgttctg tgagaccttg ggcattccct tcggcgagac ccaggcgggc aagggagagg
     1501 tgccctggga caacccgtac aacctcggcg gcattggggt gaccggcacg caggcggcca
     1561 acgcgcttgc caagagcgcg gacctggtga tcgccctcgg tacccgtctc tcggacttca
     1621 ccaccagctc caagtggcta ttccaggacc cccgctcccg gctcctgggc atcaacacga
     1681 acagcttcga cgcccacaag atgaacggcg cctccgtgat cgctgatgcc cgcctggcct
     1741 tggaggccct cggcgcggcc ctgaagaagt ccggctacaa gagtcagtgg ggcacggaga
     1801 tccagagcgc ccgaaaggag tggaacgccg aggtcgacaa gctgtactgc gcggaggatc
     1861 ccgccggcct ctcccagatc cgctgcctgg gggagctgaa cgagcgactc cttccgaagg
     1921 acgccatcgt agtggccgcg tccggcagcc tccccggcga catgcagcgg ctgtggcgcc
     1981 ccagagcctc cgacacctac cacatggagt acggcttctc gtgcatgggc tacgagatcg
     2041 gagcggctct cggcgccaag ctggcctgcc cggaacgcga ggtcttcgcc ctggtagggg
     2101 atggcagcta cgtgatgctg cactcggaga tcatcgccag ccttcaggag cgcgccaaga
     2161 tcaacgtgct ggtcttcgac aacggcgggt tccaggtcat cgacaacctc caggtcgccc
     2221 agggcatcga gcggttcggc aacgagtggc gccagcggga ccacgcctcg ggacgactca
     2281 ccggtccgtt cctcccgatc gacttcgccg ccaacggaga aagctatgga gcccgaggct
     2341 ttcgggccag gaccgtggag gagttccgcg cggccatcaa aggcgcgttg gcggagaagc
     2401 gcaccacggt gatcgacgcc aaggtcacgg ccaagtcgat gacgcacggc tacgggtcgt
     2461 ggtggcgggt cgggaccgcg gaggtctccc agaacccggc cgtcgagaaa gcggcgcggg
     2521 agatccacga ggaaagcgcc aaggcgcggg cgtactgact ccgtccggcc gcaaacgacg
     2581 ccgcggctgt cacggcgcga cgccagtccg ccgcgcggcc tacccgtgca ttccgatcac
     2641 gtttccgtcc gggtctttga tcgtggccca ccagccgtag ggctgcttct tcggcggatc
     2701 ggcaaacttc acgcccttgg ccttcagctc cttgcaggtg gcctccatat ccttcacctc
     2761 caggacaaag ggcgcgaacg tcccgatgga ggaaagggcg cgacggtagg tgctggagcc
     2821 gggcgctttc ggggtgggct tgtagagcac gatcaccgtc ttgctccctt tcggcgccac
     2881 ctcgatccaa tgaggcgcgt caggccccat tggctgatcg gtgcgcttct cgaatccgag
     2941 cttgtcgaca tagaaggcga gcgccttctg ctgatccttg acgaacaggg tgacgttggc
     3001 cacgtgggtt atcatgcgcg tctcctgtcg ccagcctacc ctacgggcaa caagcgcgca
     3061 acccgatccg gttcgagccg gggtcccagc gtcggcgcaa tccgggcgct agaccttctg
     3121 agagtccttc gccgggccgg ccggtcgatc aaccgcgagc agcgacaagc accccccaat
     3181 cacgtacgcc cccgcgcaca cccagctccg catcgccaga agcccaaggc cggccgccaa
     3241 ggcaaccaga ctgaaaacca ctttccactt gcggtctgtg tcttccgcgc aggcaagaag
     3301 gccgagggca ccgcccacca gaacgagcaa caggtttccc gcgatagagg cgatattgga
     3361 gaaggtcttt gctgtggcct gttcgagacc cttttgggct acggagtagt tcccttggag
     3421 ttgcgcccca ctgcccctta acgttgccat gatcgtgtcg ccaagggcga cggtggctat
     3481 cctcgaaagg cctcccaggc agccgagcac aagtccaatg atcaggagca ctttggacag
     3541 cgttttcatt tcacttcctc cttggacacc gccgtcccac ggtcggttcc gacggcggtg
     3601 actttttatg ccactctatc tgaacgccga gcccgaggcg ctgtcaacac gctggccgct
     3661 gtcgaggagc ccatgaggcg cccgccctca gaactccctc aggctctcgt cgattccctt
     3721 ccgcatctcg aaccaccagt ccagggcggc ttcgttcttg tccttgtcga agcggtccag
     3781 ccaatccacg agctgcgggt ccgtctcccc ttccacgtcc cgaatccgcc gcaggtaggt
     3841 gtgcaggaag tcgacgttgc gctcggattc ccaatagacg gaggcgttgc ggctgttgat
     3901 ccggctggcc gtcacgcgga tgccggcgag cagctcatcc ttgagtccgt agaggtttcc
     3961 aaagatctcc ggtatgagct cctccgccca cccgcgatgg aatcgacaga agcctgccgt
     4021 gtcgatggcc agctccttct ccattcgctc cgcgcacatc cggcccaagg tcctgggcgg
     4081 cgcgaaggcg ttgccgtagt acatgtagta cttgcccatc acgcccatgg gcgccagcac
     4141 gccaggggtc caatactggt tggggaccat ccagccttgc cggccgaacg cgacgtatag
     4201 gaaccgatcc aggagacgct tgccggtctc ccgagacaac cgcctccccc acttccgcgc
     4261 cccgtccgca aggtccactt tcccgcgtcg ctcgatgatc gactggagca gctccagccc
     4321 gatggcggcg ttgcgggcgg aatccgcgac cgggtcgaac ccgtccgcct tccagctcgg
     4381 caccccgctg acgccgagat ctgccggcgt gaggaggcct tccacgagca gctccatcat
     4441 ccaggccagc accccaccca cggagatggc gtcgaatccc aggaggtccg cctggtggca
     4501 gagccgctcc gcggcccgct gatcgaacac tccgcacagc ggacccatgg cctggtaggg
     4561 ctcgtagtcc ttcttgtact ggtccctcat cttcttgcac agcgccgagc acggttcccc
     4621 gcaggtgcgc tggtgcttgg gcgcgatggt ctcctcgttg aactgcttga ggtagtgctg
     4681 caccaccagc cgatcgtgga gcgccagtcg gtccgcttcg gtgtcgaaga tcgtccgata
     4741 gttgaaggcg atgatccggc ccttgatgcc cgcgtagttc acgccgaagg tgccgccggt
     4801 gttgaaggac gggtcgaacc ggtacttggt cgtggcctcc atgtccttgg ccaggaggcg
     4861 cttctggtac ttggtctgga accagccgtc ggcaacggtg cggtcccgga agtcgtcgtc
     4921 cacgtaggtc ccgccataga tcaccgcggc gatcccgtgc tcctggagca tcttgctccc
     4981 catgccgccg cgcccggccc aggtgtccac ctcggagagc ttgccctggt tgatgggaac
     5041 cgacgcgatg gcccccatat ccgtccgcag cgcggcgggg ccggtggcca ggatccgcgg
     5101 gtcggtggca tagcggccgg cgaaccgtgc gtagacgtgg tccatcatgg aatagacgcc
     5161 gccgcggccc tgggcccaga ccgcgtttgc gtccaccggc tccagctcca cttccacctc
     5221 ctctcctcga gtccgattca ggtagaggac ggacggcgta gctgacttgc ccaggatggt
     5281 gaccgtgttg agccccaggt tgtcgaacac gagcccggcc ccgcccatgg aggagacgaa
     5341 aaacccgcgc cagcagggag agaaccccgt cacgaacagg cggttggagc cggggaagat
     5401 cgagcccgcc aggagcccga cgccgatgtt gagagactgg tggtgagccg agatgtgcag
     5461 acccaggtct acgggcccga agaaccgacc taccgggtat cggcggatcc ggtagaaccc
     5521 ggtcgccgcg tccaggaaga ggaccttctg atccacggtg tctccttttg ccgtcggaac
     5581 gcccgagacg ctcccggggt tgggcggcgc cgatccgctg cgccacgccg atccgctacg
     5641 gccgctccac cagctccagg atccgctgcc gcgtgaggtc gcgaagctcc gccgcggtgc
     5701 tggtcttcac ggtgcccgcg gggatcggcg cgccgacgcg aaccaggagc cgggaggagc
     5761 ggatcaccca ggagcccttg cgatgcagac cgtagaggcc cgagatgcca acgggcacca
     5821 cctcgacccc tgcctctcgg gcgatatgga agggaagccg cttgaacggc cgaagctccc
     5881 cgtcggcggt ccggtgcgcc tccggcatga tcaccaggga atggcccgcc tgcaaccgcc
     5941 gcttggcctt ctgaaggctc cgcatggacg cgaatgcgtc atcccggtcg atggggatgt
     6001 tgccgatcct gcggatcatg agaccgtaca gaggccagcg gaagtgaggg ccccactcca
     6061 cgccccggac catccccggg aggtagccgc acaaaagcgg cacgtcgaag aggctcacgt
     6121 ggttgcagag gtagaggcgc ccggcgcgct ggggcgccag gtgtcccgtc ccgcggacgc
     6181 gcacgggaat caccagcacg cggaagagcc cgcgcaccat ggccttgaag aaccgctcgt
     6241 aggcaccggc ctttgcgaag atcgagcaga cgatggtcgt gacgcagatc aacaccaccc
     6301 aggaaaagcc gatgacccag cggtaggcgg aggagatcat ctgcagaaaa ccgcgatgtc
     6361 gcgggccagg cactcgatgc tgaccggagt cttgcccagg agctcgccgt ccggcgtcag
     6421 caccttgggc gtttccgtgg tgatcaggat cttgcgcgct ttgaacgtga tgacctcggg
     6481 cagacggacg tgctctcccg tgaagatgag cggaaaacag ctcaggagcc ggcgacggtt
     6541 gagcctggag agcagcgtga cgtccaggag gccgtcatcg atgctggccg ccggcgccat
     6601 gaggaagttg gaagtatacc gggtgttgga gatctccgcg aagatgctct cggtgtccac
     6661 cacgcgcccg tcggcctcca ggtggacgcg gaagggccgc aggaacaccg tccgaaggat
     6721 taccccgatg gtgtaggaca cgttccccag gagcttgagc cggctcgcgg caagggttac
     6781 atccgccacg aaccccaggc cgaggatgtt caagtagtac atcacctcgc cctgcgatcg
     6841 atagcggccc acgtccacgc gccgagtctt cccggcggct atcaccgcca cggcctcgcg
     6901 ccatcgggcg gcatccaggt cgaggtcccg ggcaaaggcg ttccccgtgc cgaccgggag
     6961 cacacccaat ggcgggcggg tggtcgaggg attgcggtag tagccgttca ccgtttcgaa
     7021 gagcgtgccg tcgccgccgg cggccaccac cccctgatac cgttccagcg gcgcctcggc
     7081 cgtgatgtgc atggcgtctt cggggccgcc ggtcagccgc tcgtcgatgt ccaccttctc
     7141 ctgctggaag gcctcgcgga ccaggggcag cgccgtgccg gcccttccat gagccgcgtt
     7201 ggggttgtag accaggagga tcctcacgcc gtgtactccc ggctttcgcc gcgatccagc
     7261 aggtccgaga gggtccgctg agggtccgcc atccgggagg tgaggatcat ggcggcgata
     7321 atgtacggct tccaccccgc ctggcggtac agcggcgagc ggaagcgctc gaacacgtcc
     7381 gcggcgacct ccccttgcgc gcagctcacg ccggtgatgt ccgccggcag gcagtggagg
     7441 tacagggcct tgtcggtgcg cttcagcagg ctctccgtgc actcccagtc cttgaagcgg
     7501 gcgttgttct ccaggcagcg cttctccagc tcccgcagcc cggccgg
//
LOCUS       DASRZS010000380         4189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1364544, whole genome shotgun sequence.
ACCESSION   DASRZS010000380 DASRZS010000000
VERSION     DASRZS010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364544"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VFH83_09265"
     CDS             complement(<1..1166)
                     /locus_tag="VFH83_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET6486595.1"
                     /translation="MRDLQDFDHILIKLASPDQIRAWSYGEVKKPETINYRTLRPEKD
                     GLFCERIFGTTKEWECYCGKFKSIRYKGVICDRCGVEVTHFKVRRERMGHIELAAPVA
                     HIWYYRSVPSRIGLFLDLQIAALRSILFYEKYIVIDPGDTELKKLQILTEEEFLEARE
                     RYGMSFTAGMGAEAIRTLLEGVDIDTLAEELRRKMIDKGPKADKRLLKRIELVEKFRA
                     STNKPEWMILEVIPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLVLNA
                     PDIIIRNEKRMLQEAVDALFDNSKKKRVVKGASNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKIWQCGLPTKMALELFKPFIMKKLVEKDVVYNVKKAKTL"
     gene            complement(1190..>4189)
                     /gene="rpoB"
                     /locus_tag="VFH83_09270"
     CDS             complement(1190..>4189)
                     /gene="rpoB"
                     /locus_tag="VFH83_09270"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET6486596.1"
                     /translation="RIIPYRGSWLEFEIDQKKELIYAKIDRKKRILATIFLRALGFET
                     RESIIELFYKVEKAKISDNREDKEKLVGRNFARGVKVKDGAGEKKLYRAGDKVHPHDV
                     EELVHAGVKEIFLIDFEHEKSLHSPIILNCFEREEMKFTKESADEQDEISKDDAILRV
                     YSVIKPGEPITIENAEKDLHSMFFSSRRYDLGRVGRYKLNKKFDYKEQVKTHTLIKED
                     IINTMTWLIRVYIGEMNVDDIDHLGNRRVRSVGELLTNQLKVAFTRMERIAKERMSLK
                     EADTIKPQDLISIKPIVATIKEFFGSSQLSQFMDQVNPLAELTHKRRLNALGPGGLSR
                     DRAGFEVRDVHYTHYGRMCPIETPEGPNIGLIVSLANYTRVNEYGFLETPYRKVKNGK
                     VTNEIEYLSAMDEERYHIAQANAALSPKGEFLEKQVSVRKGGDYITRGPEDIQYMDVS
                     PKQIISVSASLIPFLEHDDANRALMGSNMQRQAVPLMAPEPPRVGTGMEGKTAYDSGV
                     MVIAKRGGVVSYVTSLEVRIKPDDAKREEDVYQMLKYQRTNQDTCYTQKPIVSLGQKI
                     ANGQVIADGPATYQGELALGRNLLCAFMPWNGYNYEDAILVSEGVVEDDAFTSIHIKE
                     FSVEMRETKLGPEKITRDIPNTNEHAFEQLDEEGIIRVGAMVKAGSILVGKVTPKSET
                     DSTPEFKLLNSIFGEKAKEVRDTSLRVPHGVEGTVIDVQRLKRTDGDELDPGVEEVVK
                     VLIAAKRTLQEGDKLAGRHGNKGVVARVLPVEEMPYMEDGTPIDIVLNPLGVPSRMNL
                     GQIMETELGWAASALDEWYSTPVFQSASNEMIESKLKEAQLPTSSKVTLLDGQSGEPF
                     ENQITVGTIYMLKLHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRLGEMEVWALE
                     AYGASNTLQELLTIKSDDMMGRAKIYESIVKGEISTAPDIPESFNVLVQEMRGLALDV
                     SIFDVKNKQIALTERDEELIDRQMSRF"
BASE COUNT          647 a         1308 c         1321 g          913 t
ORIGIN      
        1 agcgtcttcg ctttcttgac gttgtagacg acgtcttttt cgacgagctt tttcatgatg
       61 aagggcttga agagctcgag cgccatcttg gtcgggagcc cgcactgcca gatcttgagc
      121 tcggggccga cgacgatgac cgagcgcccc gaatagtcga cgcgctttcc gaggaggttc
      181 tgcctgaacc gcccctgctt tcccttgagc atgtccgaga gggacttgag gggccgattg
      241 gaggcgccct tcacgacgcg ctttttcttc gagttgtcga agagggcgtc caccgcctcc
      301 tggagcatcc gcttttcgtt gcggatgatg atatccggcg cgttgaggac cagcagcctc
      361 ttcaggcggt tgttgcggtt gatcacgcgg cggtacaggt cgttcaggtc cgaggtggcg
      421 aaccggccgc cgtcgagctg gaccatgggc cgcagctcgg gcgggatgac ggggatgacc
      481 tccaggatca tccactcggg cttgttcgtg gaggcccgga acttctccac aagctcgatc
      541 cgcttgagaa ggcgcttgtc tgccttgggc cccttgtcga tcatcttgcg gcgaagctct
      601 tccgccagcg tgtcgatgtc cacgccctcc agcaacgtgc ggatggcctc ggcgcccata
      661 ccggcggtga aggacatccc gtagcgctcc cgcgcctcca ggaactcctc ctccgtgagg
      721 atctggagct tcttcagctc cgtgtcaccg gggtcgatga cgatgtactt ttcatagaag
      781 agaatggacc gcagggcggc gatctggagg tccaggaaga gcccgatcct ggacggcacc
      841 gaccggtagt accagatgtg cgccacgggg gccgccagct cgatgtgtcc catccgctct
      901 cggcggacct tgaagtgcgt gacttcaacg ccgcaacggt cgcagatgac tcccttgtag
      961 cggatggatt tgaacttccc gcagtagcac tcccactcct tggtggttcc gaagatccgc
     1021 tcgcagaaga ggccatcctt ctcgggacga agcgtccgat agttgatggt ctcgggcttc
     1081 ttcacctcgc cgtaggacca cgcccggatc tggtccgggg acgcgagctt gatgagtata
     1141 tgatcgaaat cctggagatc tcgcatagtc gcctccgttc agcgcccggc tagaatcgac
     1201 tcatctgccg gtcgatgagc tcctcatcgc gctcggtcag cgcgatctgc ttgttcttca
     1261 catcgaagat actcacgtcc agcgccagac cgcgcatctc ctgcaccagc acgttgaagg
     1321 actccggaat gtccggtgcg gtggagatct cacccttgac gatgctctcg tagatcttgg
     1381 cccggcccat catgtcgtcg ctcttgatcg tcaggagctc ctgaagggta ttggacgcgc
     1441 cgtaggcttc cagggcccac acctccatct ctccgagccg ctggccgccg aactgcgcct
     1501 tgcctcccag cggctgctgg gtgaccaggg agtacgggcc cgtggagcgc gcgtgcatct
     1561 tgtcgtccac caggtggtgg agtttgagca tgtagatggt ccccacggtg atctggttct
     1621 cgaagggctc cccgctctgg ccgtccagca gcgtcacctt cgagctggtg ggaagctgag
     1681 cctccttcag cttggactcg atcatctcgt tggaggcgga ctggaacacg ggcgtgctgt
     1741 accactcgtc cagcgcgctg gccgcccatc ccagctcggt ctccatgatc tgacccaggt
     1801 tcattcgcga ggggacgccc agggggttca gcacgatgtc gatgggcgtg ccgtcctcca
     1861 tgtagggcat ctcttccacg ggcagcaccc gggccacgac gcccttgttc ccgtggcggc
     1921 cggccagctt gtcgccctcc tggagggtgc gcttggccgc gatgaggacc ttgaccactt
     1981 cctccacgcc ggggtccagc tcgtcgccgt ccgtccgctt gagacgctgc acgtcgatga
     2041 ccgtgccctc cacgccgtgg gggacgcgaa gggaggtgtc ccggacttcc ttggccttct
     2101 cgccgaagat cgagttcagg agcttgaact cgggcgtgga gtcggtctcg ctcttggggg
     2161 tgaccttccc caccaggatg gacccggcct tcaccatggc gcccacccgg atgatgccct
     2221 cctcgtcgag ctgctcgaag gcgtgctcgt tggtgttggg gatgtcccgc gtgatcttct
     2281 ccggccccag cttggtctcc cgcatctcga cggagaactc cttgatgtgg atggaggtga
     2341 aggcgtcgtc ctccacgact ccctccgaaa ccaggatggc gtcctcatag ttgtacccgt
     2401 tccagggcat gaacgcgcac aggaggttgc gacccagggc cagctcgccc tggtaggtgg
     2461 ccggaccatc ggcgatgacc tgaccgttgg cgatcttctg ccccaggctc acgatcggct
     2521 tctgcgtgta gcaggtgtcc tggttggtcc gctggtactt cagcatctgg tacacgtcct
     2581 cttcccgctt ggcgtcatcc ggcttgatcc ggacctcgag ggacgtgacg tagctcacca
     2641 cgccgccgcg cttggcaatg accatgaccc cggagtcgta cgcggtcttg ccttccatgc
     2701 cggtccccac ccgaggcggc tcgggagcca tgagaggcac ggcctggcgc tgcatgttcg
     2761 agcccatgag ggcgcggttt gcgtcgtcgt gctccaggaa cgggatcagg gaggcggaca
     2821 cggagatgat ctgcttgggg gacacgtcca tgtactggat gtcctcgggc ccgcgcgtga
     2881 tgtagtctcc gcccttgcgc accgagacct gcttctccag gaactctccc ttgggagaaa
     2941 gggccgcgtt ggcctgggcg atgtggtagc gctcctcgtc catggcggag aggtattcga
     3001 tctcgttggt gaccttgccg ttcttcacct tgcgatacgg ggtctccagg aagccgtact
     3061 cgttgacccg agtgtagttg gccagggaca cgatgagccc gatgttcgga ccttcgggcg
     3121 tctcgatcgg gcacatccgg ccgtaatggg tgtagtggac gtcgcgcacc tcgaaccccg
     3181 cgcggtccct ggacagaccg cccgggccca gcgcgttcag acggcgctta tgggtcagct
     3241 ccgccagcgg gttgacctgg tccatgaact gggagagctg gctggagccg aagaactcct
     3301 tgatggtcgc cacgatgggc tttatcgaga tgaggtcctg cggcttgatg gtgtccgctt
     3361 ccttcagcga catgcgctcc ttggcgatgc gctccatgcg ggtgaaggcg accttcagct
     3421 ggttggtgag gagctcgccg accgagcgca cgcgcctgtt gccgaggtgg tcgatgtcgt
     3481 cgacgttcat ctccccgatg tagacccgga tgagccaggt catcgtgttg atgatgtctt
     3541 ccttgatgag cgtgtgggtc ttcacctgct ccttgtagtc gaacttcttg ttgagcttgt
     3601 agcggcccac gcgccccagg tcgtagcggc ggctggagaa gaacatcgag tgcaggtcct
     3661 tctccgcgtt ctctatggtg atgggctccc ccggcttgat gaccgagtag acgcggagga
     3721 tggcgtcgtc cttggagatc tcgtcctgct cgtccgcgct ctccttggtg aacttcatct
     3781 cctcgcgctc gaagcagttg aggatgatgg gagagtgcag cgacttctcg tgctcgaagt
     3841 cgatgaggaa gatctccttc acgccggcgt gcacgagctc ctccacgtcg tgcgggtgca
     3901 ccttgtcgcc ggcgcggtag agcttcttct cccccgcccc gtccttcacc ttgacgccgc
     3961 gtgcgaagtt gcggccgacg agcttctcct tgtcctcgcg gttgtcggag atcttcgcct
     4021 tctccacctt gtagaacagc tcgatgatgg actcgcgcgt ctcgaacccg agggcgcgga
     4081 ggaagatcgt ggcgaggatc ctcttcttgc ggtcgatctt cgcgtagatg agctccttct
     4141 tctggtcgat ctcgaactcc agccaggagc cgcggtaggg aatgatgcg
//
LOCUS       DASRZS010000381         6413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4057171, whole genome shotgun sequence.
ACCESSION   DASRZS010000381 DASRZS010000000
VERSION     DASRZS010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4057171"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VFH83_09275"
     CDS             complement(<1..1106)
                     /locus_tag="VFH83_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486597.1"
                     /translation="MGDDNEVRDLAKIAKEVFLQDAEHIEAEKPKLYDMEQEFARTKA
                     NRSFRTILVTLAFVALAIGATIFLTIYIENLNKRIKIDIADFEDVTLKDLLSSARKNE
                     DDLVNAKNAMQDTQNEIATKSQTITTAIAGKISVLDAEGLEKSDYDAQVAKLKDDEKN
                     QQAALRASYATVLRQRQAAIDSAQKAIDAYDSKQMAAAKKQEEVLNNEQRLHDLELAK
                     TKDQYETQIRALKEGHAQEIADLKATQERVVDAMKTKYANDVAALTLRYNPVFDTTPL
                     KNILALPIDAKLTLILGDLTTRLTAGKYVDPKVLSGIQDTLNRRTALYERIAQIPYRN
                     SIPSALAHLDFLDRSIIRSHEDVAGTLLSIIQAD"
     gene            complement(1085..2734)
                     /locus_tag="VFH83_09280"
     CDS             complement(1085..2734)
                     /locus_tag="VFH83_09280"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6486598.1"
                     /translation="MPLVTKIVLIFVVLILVSNFCSNYINLMFNRSELIVETKELLVK
                     DLKDVYNFASSQYEIFQFTNDAQKSMDSIQQAALRDLKNSKAVVLGVKADGTLGFQAS
                     QLPPLSRLTDQAAVTLMTGQRNEQGEGTLYLTLYGSSYFGVYKYNAKWDMVILRAEDL
                     NQLYAPTRQIFFNVSIIIVALTLIAALVGVALISYILRFVGIITTAIMNMRKATQMEI
                     IDLPRAPNDEITFLGVAFNALSSTVNTLVTIFRKFVSKDLALKAYREMQIRLEGSRQE
                     LTILFSDIRSFTTMTETLGTDIIKLLNLHYERAIGAIVQNDGIIGSLIGDAVLAVYGT
                     FAESHQSKTLSAIKSAYLIQENAAELRKHMAARREQIIRERGGLSEEEERIYRAVLLE
                     VGVGIDGGEVFYGNIGSQERMTNTVIGDNVNSASRMEGLTRIYNVPVIVSEYAKNDVE
                     SAVPDHGIRFLEIDKVQVKGKTEGKRVYWAILEKNLTEEVEADLEIFAKGLQLYYEGE
                     WSQAYKQFASCALPLANVFKDRTRGATRPKDWNGVWAMTTK"
     gene            complement(2950..3291)
                     /locus_tag="VFH83_09285"
     CDS             complement(2950..3291)
                     /locus_tag="VFH83_09285"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6486599.1"
                     /translation="MQASRRRSERVVISQLIDMSLMRERDIVARTININEGGILCESE
                     NPVEPLSPVYLMVRIPTTGPEYLLRTEGVVVHSRMEGDRCLFGISFSPLLERDRQAVR
                     DYLSLIPAEQT"
     gene            3512..4861
                     /locus_tag="VFH83_09290"
     CDS             3512..4861
                     /locus_tag="VFH83_09290"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6486600.1"
                     /translation="MAVPSTRRSPKLALFGGPRSVTRDPGDLFDWPIITTEEEEAVLD
                     VLRRRAMSGQDVTMKFEQEFAAWQGSRYALGYSTGTAAIQAAMYACGVGVGDEIVCQS
                     LTYWASALQCFSLGATVVFAEVDPRTLCLDPADLERRISDRTKAIVVVHYLGHPADMD
                     PILEIARRRRIRVIEDVSHAQGALYKGRKVGTIGDIGAMSLMSGKSLAVGEAGMLTTD
                     DRGLYERAIAFGHYERFDESIESPELRPYLGLPLGGYKYRMHQMSSAVGRVQLRHYDG
                     RMAEIQRAMNYFWDGLEGTPGLIPHRPAQGSGSTMGGWYAAHGILDPDALGGLSASRF
                     CEALRAEGVEVAAPGCNRPLHLHPLFNECDVYGHGKPTRIANACRDVRQPPGSLPVTE
                     RMITRIYWIPWFKHFRPELIDQYIEAYRKAALAHQDLLPGDAGDPPSVGGWHFFAHR"
     gene            4880..5782
                     /locus_tag="VFH83_09295"
     CDS             4880..5782
                     /locus_tag="VFH83_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6486601.1"
                     /translation="MYYTGFADEAARDIDTQIRATRELGWKHIESRAVDGINITDVSD
                     EAFDRIAERLRASGVSISCFGSAVANWGKDPRKQEDFDRSVAELSRALPRMKKVGCGM
                     IRGMSFTRVRDQRPDAPEIEREVFRKLTVLVRMCEEAGVLYLHENCANYGGLSYEHTL
                     KLLDAITSKSFALVYDTGNPATTFDHRGPQPYKLQSSWEFYRNVKPFIRSVHIKDGTF
                     LRATDGVFAEARYTWPGEGNGDVRRIVADLLAGGYDGGFSIEPHMAAVFHDPSKQSPQ
                     DAAYWTYVEYGKRFMKLVEECREE"
     gene            complement(5814..>6413)
                     /locus_tag="VFH83_09300"
     CDS             complement(5814..>6413)
                     /locus_tag="VFH83_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486602.1"
                     /translation="SRCAGLGKPIFLDKPLADTFAHAEQILAEAAKRGARFFSGSSLR
                     FIPQLEEACQRVPAPTCASVYGPLGKAPAGSSIVWYGVHAFEMLQRAMGNGARKVLTR
                     KDDKGAVVLVRYQDGRRGIVELNDGAWIYGGTLRTQTMARPFTADLTRMYPDLLTRIV
                     TFFQGGSAPVTLEATREIMAMLDAAERSLTSGVEEATGL"
BASE COUNT         1076 a         2014 c         2118 g         1205 t
ORIGIN      
        1 tcgtcggcct ggatgatgga caggagcgtg ccggccacgt cctcatggga ccggatgatg
       61 gatcggtcca ggaagtccag gtgggccagc gcggacggga tcgagtttcg gtaggggatc
      121 tgggcgatgc gctcgtacag cgcggtgcgc cggttcaggg tgtcctggat cccgctcagc
      181 accttggggt ccacgtactt gccggccgtg agccgcgtgg tgaggtctcc caggatgagg
      241 gtgagcttgg cgtcgatggg cagcgccagg atgttcttga gcggcgtggt gtcgaagacc
      301 ggattgtacc tcaacgtcaa cgcagcgaca tcgttggcgt acttggtttt catggcgtcg
      361 acgaccctct cctgggtggc cttgaggtcc gcgatctcct gcgcgtgacc ctctttcagg
      421 gcccggatct gcgtctcgta ctggtccttg gtcttggcga gctccaggtc atggaggcgc
      481 tgctcgttgt tcaggacctc ttcctgcttc ttcgccgccg ccatctgttt gctgtcgtag
      541 gcgtcgatgg ccttctgggc cgagtcgatg gctgcctgac gctgacggag caccgtggcg
      601 tagcttgcgc gcagcgcggc ctgctggttc ttctcgtcgt ccttgagctt ggccacctgg
      661 gcgtcgtagt ccgacttctc cagaccctcc gcgtccagga ccgagatctt gccggcaatg
      721 gcggtggtga tggtctggct cttggtggcg atctcgttct gcgtgtcctg catcgcgttc
      781 ttggcgttga ccaggtcatc ctcgttcttg cgggccgagc tcaggaggtc cttgagggtc
      841 acgtcctcga agtccgcaat gtcgatcttg atcctcttgt tgaggttctc gatgtagatc
      901 gtgaggaaga tggtggcgcc gatggcgagc gccacgaagg ccagggtcac caggatggta
      961 cggaagctgc ggttcgcctt ggtgcgcgcg aactcctgct ccatgtcgta gagcttgggc
     1021 ttctccgcct cgatgtgctc cgcgtcctgg aggaagacct ccttggcgat cttcgcgagg
     1081 tctcttactt cgttgtcatc gcccatacac cgttccaatc cttggggcgc gtcgcgccgc
     1141 gggttcggtc cttgaacacg ttggccaggg gcagggcgca gctcgcgaac tgcttgtacg
     1201 cctgggacca ctcgccctcg tagtatagct gcaggccttt ggcgaagatc tcgaggtcag
     1261 cctccacctc ctccgtgagg ttcttctcca ggatggccca atacacccgc ttgccttccg
     1321 tcttgccctt cacctggacc ttgtcgatct cgaggaagcg gatgccgtgg tccgggacgg
     1381 cggactccac gtcgttcttc gcgtactccg agacgatgac cggcacgttg tagatccggg
     1441 tgagtccctc catgcgggac gccgagttca cgttgtcccc gatgaccgtg ttggtcatcc
     1501 gctcctggga gccgatgttg ccgtagaaca cctcgccgcc gtcgatcccg acgccgacct
     1561 ccagcagcac cgcccggtag atccgctcct cttcctcgct gagcccgccg cgctcccgga
     1621 tgatctgctc ccgccgcgcc gccatgtgct tgcgcagctc ggcggcgttc tcctggatga
     1681 gatacgcgga cttgatggcg gacagcgtct tgctctggtg ggactcggcg aacgtgccgt
     1741 agacggccag caccgcgtcc ccgatcaagg agccgatgat cccgtcgttc tggacgatgg
     1801 cgccgatggc ccgttcgtag tgaaggttga gcagcttgat gatgtccgtg cccagggtct
     1861 cggtcatcgt ggtgaagctt cggatatccg agaagaggat ggtgagctcc tgccgcgagc
     1921 cttccaggcg gatctgcatc tcgcggtagg ccttgagggc gaggtccttg ctcacgaact
     1981 tgcggaagat cgtcaccagc gtgttgaccg tgctggagag cgcgttgaac gccacgccca
     2041 ggaaggtgat ctcgtcattc ggggcccgcg gcaggtcgat gatctccatc tgggtggcct
     2101 tgcgcatgtt catgatggcc gtggtgatga tcccgacgaa ccgcaggatg tagctgatca
     2161 gcgcgactcc cacgagggcc gcgatcaggg tcaaggcgac gatgatgatc gacacgttga
     2221 agaagatctg gcgggtcggc gcgtagagct ggttcaggtc ctcggcgcgc aggatgacca
     2281 tgtcccactt cgcgttgtac ttgtagacgc cgaaatagga agacccatag agggtcaggt
     2341 agagggtccc ttcgccctgc tcgttgcgct ggccggtcat gagggtcacg gcggcctggt
     2401 ccgtgagccg agacagggga ggaagctggg acgcctggaa gcccagggtg ccgtccgcct
     2461 tcacgcccag gacgacggcc ttcgagttct tgaggtcccg cagggcggcc tgctggattg
     2521 aatccatgga cttctgcgca tcgttggtaa actggaagat ctcgtactgg ctgctggcga
     2581 agttgtagac gtccttcagg tccttgacga gcagctcctt ggtctcgacg atcagctcgg
     2641 accggttgaa catgaggttg atgtagttcg agcagaagtt ggagaccagg atgagcacga
     2701 cgaagatcag aacgatcttc gtgaccaggg gcaccactcg ggtggagcca atcttcctac
     2761 ctaagatact ttccagaggc ccggccattt ccgtatcctc acgaacaacg ttaggttaac
     2821 ttgcctggga caagaaagta tacgcctagg taaccgagga gtcaattggc gatcggggaa
     2881 ggtaacggcg agtggccctg atgggctcag ggccaccgaa ggatggatgc gcggagggcc
     2941 gcggcggggt caggtctgtt cggcggggat caggctgaga tagtcccgga ccgcctgtcg
     3001 gtctctctcc aacagagggg agaacgagat tccgaacagg caccgatcgc cctccatgcg
     3061 ggaatggacg accactccct cggtcctcag gagatactcg ggacccgtgg tggggatccg
     3121 caccatgagg tagaccgggg acagcggctc cacggggttc tcgctctcgc agagaatccc
     3181 gccctcgttg atgttgatcg tgcgagccac gatgtcccgc tctctcatca ggctcatgtc
     3241 gatgagctgg ctgatgacca ctcgctcgga tctgcgccgt gatgcctgca tttcacccac
     3301 ctctggttct ctccctgtgt ccctgcaacg cacgcaacga tagccgtgat tacccagaag
     3361 tatagcgcgg acccgggaaa tctcacgcgc ggcgggaaga aaaggaccga cggggcgcgc
     3421 cgcggggctt gatctcaaac ggtgggttgc ctacaactgg agtcgcctcc acgtggcttc
     3481 ggccacgcgt gccggaaagg agccaccatc catggctgtg ccttcgacgc ggcgttcccc
     3541 gaagcttgcg ttgttcggcg gtccacgctc ggtcacgagg gatccgggcg acctgttcga
     3601 ctggcccatc atcacaacgg aagaggaaga agcggtcctg gatgtcctgc gtcgccgggc
     3661 gatgtccggg caggacgtga ccatgaagtt cgaacaggag ttcgccgcct ggcagggcag
     3721 ccgatacgcg ctgggctatt ccaccgggac ggcagccatc caggcggcga tgtacgcctg
     3781 cggggtgggc gtgggagacg agatcgtctg ccagagcctc acctactggg catccgccct
     3841 ccagtgcttc tccctcgggg ccaccgtggt cttcgccgag gtggaccccc ggacgctctg
     3901 cctggatccg gcggacctgg agcggaggat cagcgatcgg accaaggcca tcgtcgtggt
     3961 ccactacctg ggtcaccccg ccgacatgga ccccatcctg gagatcgccc gccgccgacg
     4021 tatccgggtg atcgaggacg tctcccatgc tcaaggggcg ctctacaagg gacgcaaggt
     4081 cggcaccatc ggggacatag gcgcaatgtc cctgatgagc ggcaagtccc tggccgtcgg
     4141 tgaggcgggg atgctcacca cggacgaccg cggcctctac gagcgggcca ttgccttcgg
     4201 gcactacgag cggttcgacg agagcatcga gagccccgag cttcgtccct atctcgggct
     4261 gccgctgggc ggctacaagt accggatgca tcagatgagc tccgccgtgg gccgcgtgca
     4321 gctccgccac tatgacggaa ggatggccga gatccagagg gccatgaact acttctggga
     4381 cgggctggag ggcacgccgg gcctcatccc ccatcggccg gcccagggat ccggaagcac
     4441 catgggcggc tggtatgcgg cccacggcat cctggatccg gatgccctgg gcggcctctc
     4501 cgcctctcgg ttctgcgagg cccttcgcgc ggaaggggtt gaggtggcgg cgccggggtg
     4561 caaccgtccc ctgcacctgc acccgctctt caacgagtgc gacgtgtacg gccacggcaa
     4621 gccgacgcgg atcgccaacg cttgccggga tgtgcgccag ccgccgggca gcctcccggt
     4681 gaccgagcgc atgatcacgc ggatctactg gatcccgtgg ttcaagcatt tccggcccga
     4741 gctcatcgac cagtatattg aggcgtatcg gaaggcggcg ctggcccacc aggacctgct
     4801 ccccggggac gccggcgatc cgccgagcgt cggcggctgg cacttcttcg cgcaccgcta
     4861 gtatcgaaag gaacgatcca tgtactacac cggcttcgcc gacgaggctg ccagggatat
     4921 cgacacccag atccgcgcta cgcgcgagct ggggtggaag cacatcgagt cccgcgccgt
     4981 ggacgggatc aacatcacgg acgtgagcga cgaggcgttt gaccggatcg ccgagcgcct
     5041 gcgcgcctcc ggcgtgtcga tcagctgctt cggctccgcg gtcgccaact ggggcaagga
     5101 cccgcggaag caggaggact tcgaccgaag cgtggccgag ctgagccgcg cccttcctcg
     5161 gatgaagaag gtgggctgcg ggatgatccg cgggatgagc ttcacacggg tgcgggatca
     5221 gcgtccggac gcccccgaga tcgagcggga ggtcttccgg aagctcaccg tgctggtgcg
     5281 catgtgcgag gaggccggag tcctgtacct ccacgagaac tgcgccaact acggcggcct
     5341 ctcgtacgag cacaccctca agctgctgga cgcgatcacg tccaagagct tcgccctggt
     5401 ctatgacacg ggaaaccccg ccacgacctt cgaccatcgg ggcccccagc cctacaagct
     5461 gcagagctcc tgggagttct accgcaacgt gaagccgttc atccggtccg tgcacatcaa
     5521 ggacggcacg ttcctgcgag ccaccgacgg ggtcttcgcg gaggcccgct acacgtggcc
     5581 cggcgaaggc aacggagacg tccgccggat cgtggccgac cttctggccg gcggatacga
     5641 cggcggcttc tccattgagc cgcacatggc ggccgtgttc cacgatccgt cgaagcagag
     5701 cccccaggac gccgcgtact ggacgtacgt ggagtacggc aagcggttca tgaagctcgt
     5761 cgaggagtgc cgggaggagt agcctcccac ccggcggacc ggagcatctg cctctagagc
     5821 cccgtggcct cctccacccc ggacgtgagg gaccgctccg ccgcatccag catcgccatg
     5881 atctcccgcg tggcctccag cgtcacgggc gcgctgccgc cctggaaaaa ggtcacgatc
     5941 cgggtcagaa ggtccgggta catgcgggtc aggtcggcgg tgaacggtct ggccatggtc
     6001 tgcgtccgca gggttccgcc gtagatccag gcgccatcgt tcaactccac gatcccgcgt
     6061 cttccgtcct ggtaacggac cagaacgacc gcgcctttgt cgtcctttcg agtcaggacc
     6121 ttccgggcgc cgttccccat ggcgcgctgg agcatctcga aggcgtgaac cccgtaccac
     6181 acgatggagc ttccggcggg tgccttgccc agagggccgt aaaccgaagc gcaggtgggc
     6241 gcggggacgc gctgacacgc ctcctcgagc tgcggaatga accgtagaga cgaaccggag
     6301 aagaaccggg ccccacgctt tgcggcttct gccaggatct gctcggcgtg cgcgaaggta
     6361 tcggccaacg gcttgtccag gaagatcggc ttgcccagcc cggcgcaccg gga
//
LOCUS       DASRZS010000382         3356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_980139, whole genome shotgun sequence.
ACCESSION   DASRZS010000382 DASRZS010000000
VERSION     DASRZS010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980139"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1187
                     /locus_tag="VFH83_09305"
     CDS             129..1187
                     /locus_tag="VFH83_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6486603.1"
                     /translation="MAQGKTRIGFVGVGGMGQCAHLKNYVTVPDCEVVALAELRPDLG
                     KAVAARYGIPNVYQDHEKMLASEKLDAIVASQPFWRHGTLIPELLKTGKPVFTEKPLA
                     ASIEMGEKVVQAAKAGGTWHMVGYHKRSDPASMYARAEIERLKATGELGKLRYVRLLM
                     PAGDWVANGFTDLIRVEGKGPELVYDPPAAGMSKETYDLYVSFVNYYIHQVNLMRYLL
                     GEPYRVTYADPSGVLLAGQSASGIACALEMSPYQTTVDWQESALVAFEKGWVKVELPA
                     PLASNRPGRVELYRDPGNGQTPETIVPQLPWVHAMRQQAMNFVKAVRGEGKPLCDGAE
                     ALEDLRVARDYIRLWKGV"
     gene            1200..2321
                     /locus_tag="VFH83_09310"
     CDS             1200..2321
                     /locus_tag="VFH83_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET6486604.1"
                     /translation="MVIQWRKAWIADEGFESAGVFDVDGDGVPDIVSGAFWYKGPDFR
                     RKYSVGPVRKEDEYYDDFSTIPMDVNGDGRLDFITGGWWGNTIRWRENPGDAKKQWPE
                     HVIAETGNVETTRAWDVDGDGAPELIPNTPGATEVAVFKLKTDGRGKGTGAFEKHVVH
                     RFTGSMRQGHGLGCGDVAGNGRMDIVLAHGWLEAPANPWSGEWRYHADWSCGSASVPM
                     LVVDVNGDGRNEIVVGQGHAYGLDWLERKGTKGDGGFEWTRHPIDPFNAQFHDLQWAD
                     LDGCGKPELVTGKRHRAHCGHEAGEWDDLGTYYFKWNGEGFTKQVISYGPIGTGKGCG
                     IHFALADLRGTGRLDVVAPGKDGLYVFFNEGAGPDPVKG"
     gene            2404..3270
                     /locus_tag="VFH83_09315"
     CDS             2404..3270
                     /locus_tag="VFH83_09315"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET6486605.1"
                     /translation="MSTIDQWEADYQRIETAIRFIEEHALRQPSLAEIAEFTGLSEFH
                     FQRLFSRWVGISPKRFLQFITKEYARKLLEGSEDVLSVTYDAGLSSPGRLHELFVTCE
                     AVTPGEVKSRGEGLTIEYGFHPSPFGRCILAQTPRGICGLSFVKEAPESTLVDELRAR
                     WPGAAIQCRPDSTQPVAARIFSFPSEADPGPLYLFVRGTNFQIQVWQALIRIPLGKAV
                     TYQDIARHIGLPGASRAVGNAVGSNPIPFLIPCHRVIRKMGEFGNYGEGPLRKKAILG
                     WEAALTLGEEAS"
BASE COUNT          618 a         1047 c         1197 g          494 t
ORIGIN      
        1 tgcccaccat cagcatcccc aagaccgtca agcgccagga ggttcccctg gacccggccc
       61 tggcaatcgc caatcggttc aacacgctgg ccaaggcgta gcggagagat cgccagaagg
      121 aggcgcccat ggcgcaaggg aaaaccagga tcggcttcgt gggggtcggc ggcatgggac
      181 agtgcgccca cctgaagaac tacgtcacgg taccggactg cgaggtggtg gcgctggcgg
      241 agctgcggcc ggatctggga aaggccgtgg cggcgcgcta cgggatcccc aacgtctacc
      301 aggaccacga gaagatgctg gcctccgaga agctggacgc catcgtggcc agccagccgt
      361 tctggcgcca tggcacgttg attcccgagc tcctcaagac cggcaagccc gtgttcaccg
      421 agaagcccct ggcggcgagc atcgagatgg gagagaaggt agtccaggcc gcaaaggccg
      481 gcgggacctg gcacatggtc gggtaccaca agcggagtga cccggcctca atgtacgcca
      541 gggcggagat cgagcgcctc aaggcgaccg gagagctcgg caagctgcgc tacgtccggc
      601 tgctcatgcc cgcgggggac tgggtggcca acgggttcac ggatctgatc cgcgtcgaag
      661 gcaagggccc cgagctggtc tatgatccgc ccgcggcggg gatgagcaag gagacctacg
      721 acctctacgt gagcttcgtg aactactaca tccaccaggt caacctgatg cggtacctct
      781 tgggagagcc ctaccgcgtc acctacgcgg atccctccgg cgtgctcctt gccggccaga
      841 gcgcgagcgg gatcgcgtgc gccctcgaga tgtcgcccta ccagaccacg gtcgactggc
      901 aggagtccgc cctggtggcc ttcgagaaag gatgggtgaa ggtcgagctg ccggccccgc
      961 tcgcgtccaa tcgccccggc cgcgtggagc tctaccggga tcccggcaac gggcaaacgc
     1021 cagagaccat cgtgccccag cttccctggg tgcacgccat gcggcagcag gccatgaact
     1081 tcgtcaaggc ggtccgaggc gagggcaagc cgctctgcga cggcgcggag gcgctggagg
     1141 acctgagggt cgcgcgagac tatatccgtc tgtggaaagg cgtgtgagga ggatccggca
     1201 tggtcattca gtggcgaaag gcgtggatcg cggatgaggg gttcgagagc gccggcgtgt
     1261 tcgacgtgga cggcgacggg gtaccggaca tcgtgagcgg agcgttctgg tacaaggggc
     1321 cggacttccg acggaagtac tccgtgggtc cggtccgcaa agaggacgag tactacgacg
     1381 atttctccac gatccccatg gacgtcaacg gggacggacg gctggacttc atcacgggcg
     1441 gctggtgggg caacaccatc cgctggcgcg agaaccccgg ggacgcgaag aagcagtggc
     1501 ccgagcacgt cattgcggag acgggcaacg tcgagaccac gcgcgcctgg gacgtggatg
     1561 gggacggcgc ccccgagctg atccccaaca cgccgggagc cacggaggtg gcggtcttca
     1621 agctgaagac ggacggccgg ggcaagggca ccggcgcgtt cgaaaagcac gtggtccacc
     1681 ggttcaccgg ctcgatgcgc caggggcacg gcctgggctg cggggacgtg gcgggcaacg
     1741 gccggatgga catcgtcctg gcgcacgggt ggctggaagc gccggcaaac ccctggtccg
     1801 gggagtggag gtaccacgcg gactggtcct gcgggtccgc cagcgttccc atgctcgtgg
     1861 tggacgtgaa cggagacggg aggaacgaga tcgtggtcgg ccagggccac gcgtacggtc
     1921 tggactggct ggaacggaag ggaacgaagg gggacggcgg cttcgagtgg acgcgccacc
     1981 ccatcgatcc gttcaacgcc cagttccacg acctgcagtg ggccgacctc gacggatgcg
     2041 ggaagccgga gctggtaacg gggaaaaggc atcgcgccca ctgcggccac gaggccgggg
     2101 agtgggacga cctggggacc tactacttca agtggaacgg cgagggcttc accaagcagg
     2161 tcatcagcta cggccccatc ggaacgggca aagggtgcgg gatccacttc gcgctggcgg
     2221 atctccgcgg cacgggacgg ctggacgtgg tggctcccgg caaggacggc ctgtacgtgt
     2281 tcttcaacga gggcgccggt ccggaccccg tgaaaggctg agcaagaacc gggttgcttt
     2341 ccgatccctg gaccctatca ttgagctgac gttcgatcgc gggcaaagga tggagcgccg
     2401 gccatgagca cgatagacca gtgggaagca gactaccaga ggatcgagac cgcgatccgg
     2461 ttcattgagg agcatgcact ccgccagccc tcgctggcgg agatcgcgga gttcacgggg
     2521 ctgagcgagt tccatttcca gaggctgttc agccgctggg tcgggatcag ccccaagcgg
     2581 ttcctgcagt tcatcaccaa ggaatacgcc cgcaagctgc tggagggctc tgaggacgtg
     2641 ctgagcgtga cctacgacgc gggtctctcc agccccggac gcctgcacga gctcttcgtc
     2701 acctgtgagg cggtgacgcc cggcgaggtc aagagccgcg gggaggggct caccatcgag
     2761 tacgggttcc acccgtcgcc gttcggccgg tgcatcctgg cccagacgcc gcgggggatc
     2821 tgcggtctct ccttcgtgaa ggaggcgccg gaatccaccc tggtggacga gcttcgggcc
     2881 cgatggccgg gagccgccat ccagtgtcgg ccggactcaa cgcagccggt ggcagccagg
     2941 atcttctcgt tcccatccga ggccgatccc gggccgctct acctcttcgt gcgggggacg
     3001 aacttccaga tccaggtctg gcaggcgctc atccgcatcc ctctggggaa ggcggtcacc
     3061 taccaggaca tcgcccggca catcgggttg cccggcgcat cccgcgccgt agggaacgcg
     3121 gtgggaagca atcccattcc cttcctgatc ccctgtcatc gggtcatccg caagatgggc
     3181 gagttcggaa actacggcga gggtccgctg cggaagaagg ccatcctggg ctgggaagcg
     3241 gcccttacgc tgggggaaga ggcgagctga ccggcgtcgg cggccgaccc atccggacgc
     3301 caaccgttcc ccgttgccgg gcgcgtcggc cgcggctata gtgccctcgt ggatca
//
LOCUS       DASRZS010000383         4272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5208250, whole genome shotgun sequence.
ACCESSION   DASRZS010000383 DASRZS010000000
VERSION     DASRZS010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5208250"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="VFH83_09320"
     CDS             complement(<1..1336)
                     /locus_tag="VFH83_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6486606.1"
                     /translation="MEEMVMGSLQETTGGPSEAAERENGSSNGTGRRGAKVPNLPRNR
                     QRMVQKSRNPLRRLLIFLKRATPFLIILAAAGLLALNTAILNAFLLGMQFIVQLVFGI
                     LTMIIQFAAIFWFMSRSRVERIRPEDPKVITFEDYWGQPNLKRLVRQWLGLLSDREQF
                     VRMGGRYINGLLLYGEPGTGKTMLAKAMAGEAGVAFLSIEGSGFRNMFWGVDTLKMMW
                     FVRKARKMARTYGAAIAYIDEIDAVGTSRGNVMGGRGGMGGMGGMGGGGLMGMGGSGA
                     LTRLLYEMDGLGTRTLPEKIKARFAKLFGRKAPERAWHVLFMGSTNRPDVLDPALVRP
                     GRFDQLIQVNPPDKAGRREIVQGYLRTIRHDQTVDVEAIVGDTAKSTPAQIMAALTKD
                     AVRIALFDERTLVSQYDIERAFLQQWMGIENPIEEMAPDQRRQIAVHEAGHAV"
     gene            1419..1700
                     /locus_tag="VFH83_09325"
     CDS             1419..1700
                     /locus_tag="VFH83_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET6486607.1"
                     /translation="MGQTANAAITVHLRYLSSVREKTGRRSEELALPAGSALTAVASW
                     LAATYALQVPGPSLMATLNGRGWSQHAAGLDQELHDGDEIALFPMLSGG"
     gene            complement(1686..2099)
                     /locus_tag="VFH83_09330"
     CDS             complement(1686..2099)
                     /locus_tag="VFH83_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HET6486608.1"
                     /translation="MRPKHDLVIKRTPVGLGLFTRNPIRKGAFVIEYTGVRVPNSDES
                     RLKGRYLFEVNSRWTIDGSGRENLSRYINHSCRPNCEAIISRGRILIYAKRAIAPDEE
                     LAYDYGKAYFDEFIKPKGCGCAHCRTQAASASSPG"
     gene            complement(2099..3031)
                     /locus_tag="VFH83_09335"
     CDS             complement(2099..3031)
                     /locus_tag="VFH83_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET6486609.1"
                     /translation="MIPVFVLALLPILVMPESSRPDLSPGRERAAARVAPGGQEVLAL
                     AAAWPDRIVETRVGDDEWMLRIGDQWFAWAHGRLLPEAERGRWRDYDPLEFYDYPTSL
                     PELSPLDEQTAARLRQYVRDEHPRRRSEAFLSRLLGAGSRAETEAHVVTMEVAGFTVT
                     VHEALKAPLTRVSDDLWALRRRDPEVAAFLRGLVEMNGYNYRFVEGTRSRSLHSYGLA
                     VDLIPRSYGRLQTYWLWALNRTSTWWTIPYDQRWMVPQPVVEAFERQGFVWGGKWLFF
                     DTMHFEYRPDLVRSARARIALGPARWGDLPSEDD"
     gene            3180..3701
                     /locus_tag="VFH83_09340"
     CDS             3180..3701
                     /locus_tag="VFH83_09340"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6486610.1"
                     /translation="MTAELCAHEALEVVPSVMRFLRVEMRSNRGSDLSVPQFRALGHL
                     RHNPGTSLSALADHLGLTAATTSNLVDGLVRRQMVSREPSPGDRRRVALALSAPGRRT
                     IDGSMAAAERNLATRLGALSEEDLRSIHRGLAALRRVFAPVPTKPGGSRLPRNARSPI
                     LDGRATLRGGGTA"
     gene            3698..4231
                     /locus_tag="VFH83_09345"
     CDS             3698..4231
                     /locus_tag="VFH83_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020615403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET6486611.1"
                     /translation="MKRIVAVVDDIYEDLELWYPRIRLEEEGWKVVVAGPEAGKVYAG
                     KHGYPCKTDISFAEMRPADYDALLVPGGFAPDKMRRDGRVLDLVRSMNDQGKLIAFIC
                     HAGWVLISAGILRGKRATSTVGIRDDMVNAGTRWVDEALVIDGNLVSSRTPADLAPFA
                     RAMVDWLQGRLAKSQAA"
BASE COUNT          689 a         1436 c         1444 g          703 t
ORIGIN      
        1 ccaccgcgtg tccggcctcg tggacggcga tctgacggcg ctggtccggc gccatctctt
       61 cgatggggtt ctcgatgccc atccactgct ggaggaacgc gcgctcgatg tcgtactggg
      121 acacgagcgt acgctcgtcg aacaacgcaa tgcggacggc atccttggtg agcgccgcca
      181 tgatctgagc cggtgtggac ttggcggtgt ctccgacgat ggcctccacg tccaccgtct
      241 ggtcgtgacg gatggtcctc aggtatccct ggacgatctc ccggcgcccg gccttgtccg
      301 gtgggttcac ctggatgagc tggtcgaacc gtcccggcct caccagagcc gggtccagga
      361 cgtccggccg gttggtcgaa cccatgaaca ggacgtgcca cgcgcgctcg ggcgcctttc
      421 ggccgaagag cttggcgaac ctggctttga tcttctccgg caacgtccgc gtgcccaggc
      481 cgtccatctc gtagagcagc cgggtgagag cgccggagcc gcccatgccc atgagcccgc
      541 ccccgcccat tccccccatg ccgcccattc cgccgcggcc ccccatgacg ttgccgcgag
      601 acgtgccgac cgcgtcgatt tcgtcgatgt aggcaatggc agcgccgtag gtccgggcca
      661 tctttcgcgc cttccgcacg aaccacatca tcttgagggt gtccacgccc cagaacatgt
      721 tgcggaaccc tgagccctcg atggagagga aagccacccc cgcctccccc gccatggctt
      781 tggccagcat ggtcttgccg gttcccggct cgccgtacag gaggaggccg ttgatgtagc
      841 ggccgcccat gcggacgaac tgctcgcggt cggagagcag ccccaaccac tggcggacca
      901 gcctcttcag gttgggctgg ccccagtagt cctcgaaggt gatcaccttc gggtcctcgg
      961 gtctgatccg ctccacgcgg gaccgggaca tgaaccagaa gatcgccgcg aactggatga
     1021 tcatcgtcag gattccgaat accagttgga cgatgaactg catgccgagg aggaaggcgt
     1081 tcaggatcgc cgtgttgagg gccaggagcc ctgccgcagc caggatgatc aggaacggcg
     1141 tcgcgcgctt gaggaagatg agcaatcgcc gcagcggatt gcgagacttc tggaccatcc
     1201 gctgccggtt ccgcggcagg ttagggactt tggctcccct cctcccggtt ccgttcgagc
     1261 ttccgttctc gcgttccgcg gcctcactgg gcccgccggt tgtttcctgg agactgccca
     1321 tgaccatctc ctccattcgg aaaaaggtag ctacaatata gttcgcgacc tgtgggaaaa
     1381 ccaaaccatc gtttgatacg gatggagggg atggctggat gggacagacg gccaatgccg
     1441 cgattaccgt tcaccttcgg tacctgtctt cggtgcgaga gaagaccgga cgccgctcgg
     1501 aggagcttgc gctgccggcg ggcagcgcgc tgaccgcggt ggcctcgtgg ctggccgcga
     1561 cctacgcgct tcaggtcccc ggaccctccc tcatggccac gctcaacggc cgcggctgga
     1621 gccagcacgc ggcgggcctt gaccaggagc ttcacgacgg agacgagatc gcgttgttcc
     1681 cgatgctatc cgggggatga cgcggaggcc gcctgcgttc tgcagtgcgc gcagccacat
     1741 cccttcggtt tgatgaactc gtcgaagtag gccttgccgt agtcgtaggc gagctcctca
     1801 tccggcgcta tggcgcgttt ggcgtagatg aggatccgtc cgcgagagat gatcgcttcg
     1861 cagttggggc ggcaggagtg gttgatgtac cggctcaggt tctctcgacc ggatccgtcg
     1921 atggtccatc gcgagttgac ctcgaagagg tagcgcccct tcagccgcga ctcatccgag
     1981 ttcggcaccc gcacgccggt gtactcgatc acgaacgcgc ccttgcggat ggggttgcgc
     2041 gtgaagagcc ccaatcctac gggggtccgc ttgatcacca ggtcgtgctt gggtcgcatc
     2101 agtcgtcttc ggaaggcaga tcgccccagc gggccgggcc cagcgcgatc cgcgccctgg
     2161 cggacctcac caggtcgggc cgatactcga agtgcatcgt gtcgaagaac agccacttgc
     2221 cgccccagac gaacccctgc cgctcgaagg cttccacgac cggctgcggg accatccagc
     2281 gctggtcgta ggggatcgtc caccaggtgg acgtccggtt cagggcccag agccagtacg
     2341 tctggagcct cccgtagctt cgagggatga gatccacggc caggccgtag ctgtggaggc
     2401 tgcgggagcg cgtgccctcc acgaagcgat agttgtatcc gttcatttcc acaaggcctc
     2461 gaaggaacgc ggccacttcg gggtcccggc gccgcaacgc ccagaggtca tcggagactc
     2521 gcgtcagggg agccttgagc gcctcgtgca ccgtcacggt gaagccggcc acctccatcg
     2581 tgaccacatg ggcttcggtc tcggcccgtg agcccgcgcc cagcagccgg gagaggaacg
     2641 cctcgctgcg tcgccgcggg tgctcgtcgc gcacgtactg gcgcaaacgc gccgcggttt
     2701 gctcatccag cggagagagc tcggggagcg aggtcgggta gtcgtagaac tccagcgggt
     2761 cgtagtctcg ccaacgccct cgctccgcct ccgggaggag cctgccgtgg gcccaggcga
     2821 accactggtc gccgatccga agcatccact cgtcgtcccc gacccttgtc tccacgatgc
     2881 ggtccggcca tgcggcggcc agggccagga cctcctggcc cccgggcgcg acgcgagcgg
     2941 cggcccgctc tcggccgggg gacaggtctg gtcgagagga ctccggcatc accaggatgg
     3001 gaaggagcgc gagcacgaaa accgggatca tgcgcccagt acttaatctc ggtccgacgg
     3061 cggagtcaag actcctcgcg ccggcgtcgc cggcggcccg gtccccggcg acccgcggct
     3121 tgacccgccc cgaagcccgt agatatcctt cgaccatcaa acaattcgag gggctaacca
     3181 tgaccgccga gctgtgcgcc cacgaggcgc tggaggtcgt accttccgtg atgcggttcc
     3241 tccgcgtgga gatgcggagc aaccgggggt cggacctgag cgtgccccag ttccgggcgc
     3301 tcggtcacct ccgccacaac cccgggacgt ccctgtcggc cctggcggac cacctgggtc
     3361 tcacggctgc caccacgagc aacctggtgg atgggctggt ccgacggcag atggtctccc
     3421 gggagccatc ccccggggac cggcgccggg tggccctggc gctcagcgcc cccggcaggc
     3481 gcaccatcga cgggtcgatg gcggccgccg agcggaacct ggccacacgg ctcggcgcgc
     3541 tctccgagga ggatctccga tccatccacc gggggctggc cgcgcttcga cgggtcttcg
     3601 cgccggtgcc cacgaagccc ggcgggagtc ggttgccgcg gaacgcgcgc tcgccgatcc
     3661 tcgatggccg ggctaccttg agaggcggag ggaccgcatg aagcgaatcg tcgccgtcgt
     3721 ggacgacatc tacgaggacc tggagctctg gtatccacgg atccgactgg aagaggaagg
     3781 ctggaaggtc gtggtggccg ggccggaggc gggaaaggtc tacgccggca agcacggcta
     3841 cccatgcaag acggacatct ccttcgcgga gatgaggccg gcggactacg acgcgctcct
     3901 ggtgcccggc ggcttcgccc cggacaagat gcgccgggac gggcgcgtcc tggacctggt
     3961 gcgctcgatg aacgaccagg gaaagctgat cgcgttcatc tgccacgccg ggtgggtgct
     4021 catctccgcc gggatcctgc gcggcaagag ggcaacgagc acggtaggaa tccgggacga
     4081 catggtcaac gcggggacgc ggtgggtcga cgaagcgctc gtcatcgacg gcaacctggt
     4141 gagctcccgc acgccggcgg acctggcgcc gttcgcgcga gccatggtgg actggctgca
     4201 gggccgcctt gccaagagcc aggcggcttg aggcccgccc tgggggcggc ttgaggccgc
     4261 cctgggggcg gc
//
LOCUS       DASRZS010000384        12154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2130660, whole genome shotgun sequence.
ACCESSION   DASRZS010000384 DASRZS010000000
VERSION     DASRZS010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2130660"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFH83_09350"
     CDS             <1..316
                     /locus_tag="VFH83_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486612.1"
                     /translation="TIRQLQYAQANNVLWLQIPMFQDIDFSKYLTIVPKQWVQPIIDS
                     VKAARPEYGGKGPVSQYIAAAISQCMAAPKANVKAILQAAQDQAYDEYLTQYNQDVLK
                     QK"
     gene            421..1314
                     /locus_tag="VFH83_09355"
     CDS             421..1314
                     /locus_tag="VFH83_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6486613.1"
                     /translation="MELRRGPAGRGPRTNWLVAAFFLGPTVAAFIAFSYLPLGRAVYM
                     SLHNYNWMKPPGPFVGLSNYLKSFRSLSFRTDLANSAILFLFSLALGFWVPILQALLL
                     DQIAGPAQRLMKYFYLLPMAIPAVASFLVWKWIWNPSLGLANAVLALAHIPPQLWLGD
                     PRLVKLTLRLPYLLGGGMNVMIILAAIQGVSPELHEAAEIDGAGAWQRMWNITVPGIW
                     HIVTILFILNLTGALLAFDDVFVMTGGGPGTASTTIVYGIYLVAFRQQNPGQGSAWAV
                     LVLVLTLIITGIQLRLRRSDS"
     gene            1311..2687
                     /locus_tag="VFH83_09360"
     CDS             1311..2687
                     /locus_tag="VFH83_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6486614.1"
                     /translation="MSHPPRNRIRKPGGDYALISVAVVVTALWVLAGLFPPVWMALSA
                     FKSSYEGTLFPPSFLPRMPQRVSVALAYDGGAVPSDAEGLLSLVRDDAFTAQWTLFDA
                     NPRNPMGEVQVSAYVDGREVYQSSLLLFSYYYGRNRIWSATILDRTQILKHKSDIQDG
                     DTIRLYEKGLRRWPVGAGPQMPPPVISANKTAADSRDTLAGMGMAGTVHSLTTSNAPL
                     RLFDGFIRGWQNVRPEVGPLRWGRYFINSAAITAGGILTQWLFTAFAAYGLSRVLRRR
                     ASGILTMFFLATMMIPAIATVIPLFLQMQRWGTINTFWSIILPAIPSGFLVYLFKGFF
                     DALPGELFEAARMDGATDFWIFLRLVIPLSTAVFSVVTLMCFLGGWNGGGGLMWSSLV
                     LPKEEMHPFPLALYYMDKTVPGYGALGQAQNQEMAMTLVASVPTLILFLFFQDRVAKG
                     LVWSGLKQ"
     gene            2684..4801
                     /locus_tag="VFH83_09365"
     CDS             2684..4801
                     /locus_tag="VFH83_09365"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET6486615.1"
                     /translation="MSDTFAGLQSAFRAPGKEHRPLQIIHGFDRAAEEGIDRYLTRLV
                     NAGLGGIVTNVSFKDYLQSPEQWDLLRRGMAAARRAGLTLWIYDEDGYPSGAAGGLVL
                     DGHPELEALAVACERRTVAGPARVEIAPPDCSLGFTGASVVGADATGGPRDVSGNADS
                     SGRFTLDLPAGDWTVLYFFRKAAYEGTHAQGNVHASRRYINVLEPRAARRFLDVTHEA
                     YRKELGPDLGGARSFFTDEPSLMSSYVWPLPPNIEGKVRIQDPLRFHDRHPMVAWSED
                     FPKRFQARKRYDILPRLACLFVDAGPDSRKVREDYREVVAELFADSYFGVIGDWCREH
                     GVESSGHLLVEESLLWHAAFLGDLFTPARRLSIPGIDMLDSDPVTILGTEGFMVPKQV
                     SSAAHLEGRSQVMSECSDWAMRNGNRPATPAQIRGTYNLLYLLGVTVITSYYAWEEIG
                     ADYAGINEHAGRLRLLLGAGRHDATVAVLYPIRGVWGRYLAQSEPPLPPTQPPEVRAI
                     VADWTSACRELLTRQVDFDCLDERAVCDGSVREGALRVGLESYNTLVIPPTDSLAPAT
                     REAIARMAGSGVRVIVLRNPVLPPIPGLDVRAAGGLVMDLSELPGLVGTVIPQAVRLR
                     TPNPAILCARHLHPEAAYCWFLVNTSAAHAAAEAALDRSGSARLWDPATGSVSEPVRY
                     SAGDWLPMELGPYAGIFVTLEGA"
     gene            4798..5628
                     /locus_tag="VFH83_09370"
     CDS             4798..5628
                     /locus_tag="VFH83_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486616.1"
                     /translation="MSAIDLFEEACALCLEGKPAQAVETLRAGIMDGLWWSPSLLDGD
                     PDLAGLRGQIEYPDMRRICESRFRSEQAAARPRLLVISPATTPWEPKTLFVIHWWGDT
                     ASSFVGPWRRLVDEGWTLVVPQSSQMCCSGSYCWDDTARALAELRQHLEDCARKRGLG
                     LEGMVLAGASQGAPLALELAHEVGAPWLCVVPSFPSGYDVSPLLGVPRRARGAFLLGE
                     QDPANARARPVMEALRGAGIPWFQTILEGVGHELPDELAVRVAEILRELRSATEGEDA
                     "
     gene            5625..6668
                     /locus_tag="VFH83_09375"
     CDS             5625..6668
                     /locus_tag="VFH83_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6486617.1"
                     /translation="MTTTGVEGAVTERRARWERFLRMDGKQAFMHLIAWESWGTERPW
                     PTAENRQARIEWAWQVYERRLGLLGTLDDDGLPFLDPYTGTEIFAQAFGCRVVYPLDN
                     MPFALPLIDKPMEVSRVQVPDLDSPSLALLFDIADELRRRAGPGALMRLIDVQSPMDI
                     AALIWNKNTFYAAMLDAPEAVIELAHKVKKLLVLFLDAWFSRYGSSFIAHYPDYYMPA
                     GLTLSEDEVGAVSPQMFERFFLPELAELSNRYGALGMHCCASARHQWQGFLKIPNLKV
                     LNICQPAGVVAQAHRFFAAICAQQHLPPESWDVSQVTPSQFPSGAHVVLTSAVKSQED
                     ALRCSAAFHRAFR"
     gene            complement(6728..8026)
                     /gene="asnS"
                     /locus_tag="VFH83_09380"
     CDS             complement(6728..8026)
                     /gene="asnS"
                     /locus_tag="VFH83_09380"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET6486618.1"
                     /translation="MDRVTIAEIGKHVDQEVEIRGWLYNLRSKGKIAFLQLRDGTGRI
                     QAVAEETACGAAAFASISALRMEASVIVRGTVRQDSRAPSGFELTLSGLEVVQNPSED
                     YPIAKKEHGIDFLLENRHLWLRSTRPAAIMRIRDQAIRSFRDFFHERGFLLIDTPILT
                     GSIGESAGTLFTVPYFDLGDAYLAQTGQLYLEAACMAYGKVYDFGPTFRAEKSKTRRH
                     LTEFWMLDAEVAYADSADNMRLQEDMVTRVVTDVLANCAADLTALERDTVPLTKVTPP
                     FVRMEYGDAVKTLQSKGSSIQWGDDLGGEDETILTKMHEKPIFVMNYPKKAKAFYMKE
                     NPANPDTVLCSDLLAPEGYGEIIGGSQREDVLEKLLARIHEEKLPEDAYGWYLDLRKF
                     GSVPHSGFGIGLERTLAWICGSQHIRECIPFPRTISRIYP"
     gene            complement(8094..9098)
                     /locus_tag="VFH83_09385"
     CDS             complement(8094..9098)
                     /locus_tag="VFH83_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HET6486619.1"
                     /translation="MGFIRETSRDAGYRKAAQFLMLLGKEEAARVLRHLAPQEVEGIT
                     REIARTQKIDERDATKVLEEFGYLSQTRALIAQGGIDKAQEMLVASVGAEKAREIIEK
                     VRKDMAPPPFTFLKDVDVHQVIALVRQESAPVAALILAHLEPKLAARILQALSPEVQR
                     EVVGRIAKMQQVSPEVLRRAEEALKSKVRDLGEPATQEVDGTQTLTAILRLMDPTREQ
                     AILSDLDPNTANQIRKMLYTIEVVFQIPDKDLQKILRDYADREIAMVLKGVSDEVGAR
                     ITSSISEHRRDLVKLESDAMGPARRADVDRAIQDFLEYIQLLEQKGEIAILREREKFV
                     "
     gene            9320..9820
                     /locus_tag="VFH83_09390"
     CDS             9320..9820
                     /locus_tag="VFH83_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486620.1"
                     /translation="MAETIALVANVALALSLVVGLVFGIAQVRAAARDRRERLTLEAL
                     RGFQSRDFVELMQYIIAHDIPKSRAEMSALPDREQVVFLQCSQAMEMLGLLVAERLID
                     LDLVERTLGSFVTTSWEKLKSITVDVRQTDPFLHEYFQWLAERIGERMSRHPRRPFHE
                     TGPRPT"
     gene            9822..11126
                     /locus_tag="VFH83_09395"
     CDS             9822..11126
                     /locus_tag="VFH83_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6486621.1"
                     /translation="MASDRSVVERVEELYRAESTRVLATLIRLLGDFDLAEESLQEAF
                     RAALEQWPKEGLPRNPRAWLVSTGRFKGIDVVRRRGRHDALLAAVAQESREAFVDPEA
                     VDDETLQDDQLRLIFTCCHPSLPQEGRIALALREVCGLTTEEIARAFLVTTATMTRRI
                     ARAKEKIRQERIPYAVPSRQELARRLATVLQVVYLVYNEGFVSTAGQALSRRDLAEDA
                     LYLGRLIVELIPEPEAIGLLGLILLHESRRPARTDANGDVIPLEDQDRSLWNREQIEE
                     GIGLAHRALLSGRFGSYTLQATIAAVHAVAPSVVETDWDQIVRLYDMLLETGPSPVVE
                     LNRAIAVAMRDGPSAGLSLIDALLERGELADYHPVHAVRADLNRRLGRVGEATASYRR
                     ALTLARQEPERRYLEKRLAEVSGSSASLSVLAVPVRLLDEIS"
     gene            11188..11601
                     /locus_tag="VFH83_09400"
     CDS             11188..11601
                     /locus_tag="VFH83_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6486622.1"
                     /translation="MNAHVSSILLGVRDMERSKRFYTEGLGWKVQNDYRISVFFVPHG
                     GSIVGFYGRDGLAASVGASPEGSGFSGVVFNYVVRSEARVDEILSEARKAGATILKPA
                     AALPWGGYGGSFADPDGYIWNVGYSAQGKDQPYAE"
     gene            11626..11949
                     /locus_tag="VFH83_09405"
     CDS             11626..11949
                     /locus_tag="VFH83_09405"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET6486623.1"
                     /translation="MQFFVYGSPSERQQPRDPAELGKFMEESVKKGILITGGGLHKKE
                     TRVVLAEGKVSITDGPFLEGKELIPGFTVIQVDTKEQAIEWVRSLRQLYGDGETKLVP
                     IFGPS"
BASE COUNT         1899 a         4059 c         4034 g         2162 t
ORIGIN      
        1 caccatccgc cagctccagt acgcgcaggc caacaacgtg ctctggctgc agatccccat
       61 gttccaggac atcgacttct ccaagtacct caccatcgtc cccaagcagt gggtgcagcc
      121 catcatcgac tcggtgaagg cggcccgccc tgagtacggc ggcaaggggc ccgtcagcca
      181 gtacatcgcc gcggccatct cccagtgcat ggcggcgccg aaggccaacg tgaaggccat
      241 cctccaggcg gcgcaggacc aggcgtacga cgagtacctg acccagtaca accaggacgt
      301 cctgaagcag aagtagccgg cgcgattcga cagagacggt tcgtgcggcc cgggcctcgc
      361 caccgagtga gctcccgggc cgcacccttt ggagggacgg gcgtgtcatc ggatcgatcg
      421 atggagctgc ggagagggcc cgccggacgg ggaccgcgga ccaactggct ggtcgccgca
      481 ttcttcctgg gcccgacggt ggcggcgttc atagcgttca gctacctgcc gctgggccga
      541 gcggtctaca tgagcctcca caactacaac tggatgaagc cgccgggccc cttcgtgggc
      601 ttgtccaatt acctgaagtc gttccgctcc ctttcgttcc gcaccgacct ggccaactcc
      661 gccatcctct tcctcttctc ccttgcgctg ggcttctggg tcccgatcct ccaggcgctg
      721 ctgctggatc agattgccgg gcccgcgcag cggctgatga agtacttcta cctcctcccc
      781 atggccatcc ccgcggtggc ctcgttcctg gtctggaaat ggatctggaa ccccagcctt
      841 ggccttgcca acgcggtgct ggccttggcg cacatcccgc cgcagctctg gcttggggat
      901 ccgaggctgg tcaagctcac cctccggctt ccctacctgc tgggcggcgg catgaacgtg
      961 atgatcatcc tcgcggccat ccagggcgtt tctccggagc tccacgaggc cgccgagatc
     1021 gatggcgcgg gcgcctggca gcggatgtgg aacatcacgg tgccgggcat ctggcatatc
     1081 gttaccatcc tcttcattct gaacctgacc ggcgcgctcc tggccttcga cgacgtgttc
     1141 gtgatgacgg ggggcggacc gggcaccgct tccaccacca tcgtctacgg catctacctg
     1201 gtggcgttcc ggcagcagaa cccggggcag gggtccgcct gggcggtcct ggtgctcgtg
     1261 ctgaccctca tcatcacggg gattcagctt cggctgcgaa ggagcgactc atgagccatc
     1321 ctccgcgcaa ccgcattcga aagcccggcg gagactacgc cctgatcagc gtcgcggtgg
     1381 tcgtgacggc cctctgggtc ctggccgggt tgttccctcc ggtttggatg gcgctctccg
     1441 cgttcaagag cagctacgaa ggcaccctct ttccaccctc gttcctcccc aggatgcctc
     1501 agcgggtttc cgttgccctg gcgtatgacg gaggcgcggt gccttcggat gcggagggac
     1561 tgctcagcct ggtgagggac gatgccttca cggcgcagtg gaccctcttc gacgccaacc
     1621 caagaaaccc gatgggggag gtgcaggtca gcgcctacgt ggatggccgc gaggtctacc
     1681 agagcagcct gctccttttc agctactact acgggaggaa ccgcatctgg tcggccacca
     1741 tcctggaccg gacccagatc ctgaagcaca agagcgacat ccaggacggg gacaccatcc
     1801 ggctgtatga gaaaggattg agacgctggc cggtcggcgc cggcccgcag atgccgccgc
     1861 ccgtcatctc cgccaacaag accgcggccg acagccggga cacccttgcc ggcatgggaa
     1921 tggcaggcac ggtccattcg ctgaccacgt cgaacgcgcc gctccgtctc ttcgacggtt
     1981 tcatccgcgg ctggcagaac gtccgtcccg aggtgggtcc gcttcgctgg ggccgatact
     2041 tcatcaacag cgcggcgatc acggcgggag gcatcctcac ccagtggctc ttcacggcgt
     2101 tcgccgccta tgggctctcc cgcgtcctga ggcgtcgggc cagcggcatc ctcaccatgt
     2161 tcttcctggc caccatgatg atccccgcca tcgccacggt gattcccctg ttcctccaga
     2221 tgcagcggtg gggcaccatc aacacgttct ggtccatcat ccttcccgcc attccctccg
     2281 gcttcctggt ctacctcttc aaggggttct tcgacgccct gccgggcgag ctcttcgaag
     2341 cggcgcggat ggatggcgcc acagacttct ggatcttcct gagactcgtg attcccctct
     2401 ccaccgcggt cttctccgtg gtgacgctga tgtgcttcct gggcggatgg aacggaggcg
     2461 gcggcctcat gtggagctcc ctggtgcttc ccaaggaaga gatgcacccc ttccccctgg
     2521 ccctctacta tatggacaag accgtgcccg ggtacggggc cctgggtcag gcgcagaatc
     2581 aggagatggc gatgacgttg gtcgcgtccg taccgacgct gatcctcttc ctcttcttcc
     2641 aggaccgagt cgccaagggg ctggtctgga gcggactcaa acagtgagcg acaccttcgc
     2701 cgggctccag tccgcgtttc gggctcctgg caaggagcac cggcctctgc agatcatcca
     2761 cggcttcgat cgcgccgcgg aggaggggat agaccgctac ctgacgcggc tggtgaacgc
     2821 ggggctgggc gggatcgtca ccaacgtcag cttcaaggac taccttcaaa gccccgagca
     2881 gtgggacctc ctgcgccgcg gcatggcggc cgcccgccgc gcggggctga cgctctggat
     2941 ctacgacgag gacggctacc ccagcggcgc tgccggcgga ctggtgctgg acggccatcc
     3001 cgagctggag gcgctggcgg ttgcctgtga gcggcggacc gtcgccggcc ccgcccgggt
     3061 ggagatcgcg ccgccggact gctcgctggg gttcacgggc gcgagcgtgg ttggcgccga
     3121 tgccaccggc ggcccacgcg acgtttcggg caacgcggac tcctcgggca gattcacgct
     3181 ggatctgccg gccggagact ggacggtgct gtacttcttc cgcaaggccg cctacgaagg
     3241 cacccacgcg caaggaaacg ttcacgccag tcggcggtac atcaacgtcc tggagcccag
     3301 agccgccaga agattcctcg acgtcaccca tgaggcgtac cgcaaggagc tcggaccgga
     3361 tcttggcggc gcccggtcgt tcttcacgga cgagccttcc ctcatgtcca gctatgtctg
     3421 gccgcttccg ccgaacatcg agggcaaggt ccggatccag gatccccttc gcttccatga
     3481 ccggcacccc atggtggcct ggtccgagga cttcccgaag aggttccagg cgcgcaagcg
     3541 gtatgacatc ctgcctcgac tcgcctgcct cttcgtggac gccggtccgg attcccggaa
     3601 ggtgagagag gattaccgcg aggtggtggc cgagctcttc gccgacagct acttcggggt
     3661 catcggggac tggtgccggg agcacggcgt ggaatccagc ggccatctcc tggtggagga
     3721 aagtctcctc tggcacgcgg cgttcctggg ggacctcttc acgcctgccc ggcggctctc
     3781 catcccggga atcgacatgc tggactcgga tccggtcacg atcctgggta cggaaggatt
     3841 catggtcccc aagcaggtgt cttccgccgc ccacctggag ggtcggtccc aggtcatgag
     3901 cgagtgcagc gactgggcaa tgcgcaacgg gaaccgaccc gccacgcccg cccagatccg
     3961 cgggacctac aacctgctgt acctcctcgg cgtcaccgtg atcacctcct actacgcgtg
     4021 ggaggagatc ggcgccgact atgccggcat caacgagcac gcgggacggc tgcgcctgct
     4081 gctgggagca ggccgccatg acgccaccgt ggccgtgctc tatcccattc gcggcgtgtg
     4141 gggccgctac ctggcccaaa gcgagccgcc ccttccgccc acccaaccgc cggaggtgcg
     4201 ggccatcgtg gcggactgga ccagcgcctg tcgggagctc ctcacgcgcc aggtggactt
     4261 cgattgcctc gacgaacgcg cggtgtgcga tggttcggtc cgcgagggcg cactccgcgt
     4321 cgggttggag agctacaaca ccctcgtgat cccgccgacg gacagcctgg ctccggctac
     4381 ccgtgaggca atcgctcgga tggccggatc cggcgtgcgg gtgatcgtgc ttcgtaaccc
     4441 cgtcctcccg ccgatccccg gcctggacgt ccgagcggcc ggcggtttgg tcatggacct
     4501 ctcggagctg ccgggccttg tcggcacggt gatccctcag gccgttcggt tacggacccc
     4561 caatccggcg attctgtgtg cgcggcacct tcatccggaa gccgcgtact gctggttcct
     4621 ggtcaatacg tccgcggccc atgcggccgc ggaggcagcc ctcgaccggt caggttcagc
     4681 gcgtctctgg gatccggcaa cgggctccgt aagcgagccc gttcggtact ccgccggtga
     4741 ctggctcccg atggagctcg ggccctacgc gggcattttc gtgacgctgg aaggggcatg
     4801 agcgccatcg acctcttcga agaggcatgc gctctctgtc tcgagggcaa gccggcgcag
     4861 gcggtggaaa ccctgcgcgc cggaatcatg gacggattgt ggtggagccc gtcgctgctg
     4921 gacggggatc ccgatctggc cgggcttcgc ggtcagatcg agtacccgga tatgcgacgg
     4981 atctgcgagt cacggttccg ctccgagcag gcggcggccc gaccgcggct cctggtcatc
     5041 tccccggcaa caacaccgtg ggaaccgaag acgctgttcg tgatccactg gtggggagac
     5101 acggcgtcgt ccttcgtcgg tccgtggagg cgcctggtcg acgaaggatg gacccttgtg
     5161 gtcccgcagt cgtcgcagat gtgctgctcc ggaagctact gctgggacga cacggccagg
     5221 gcgctcgccg agctccgcca gcacctggag gactgcgctc gcaagcgcgg gctggggctg
     5281 gaggggatgg tgctggcggg cgcctcccag ggcgcgcccc tggccctgga gctggcgcac
     5341 gaggtaggcg cgccgtggct gtgcgttgtg ccctcgttcc cgtccggata tgatgtgtcg
     5401 ccgctcctgg gggttccccg gcgcgcgcgg ggggcgttcc tgctcgggga gcaggatccc
     5461 gcgaatgcgc gcgcgcgacc ggtgatggag gccctccgcg gagccggcat cccgtggttc
     5521 cagacgattc tcgaaggggt gggtcacgag ctgcccgatg agcttgcggt ccgcgtcgcc
     5581 gagatcctgc gggagctgcg gagcgccacg gaaggggagg acgcatgacg accaccggcg
     5641 tggaaggggc cgtgacggag cgcagggccc gatgggagcg gttccttcgc atggacggca
     5701 agcaggcgtt catgcatctg atcgcctggg agtcgtgggg gaccgaacgc ccgtggccca
     5761 cggcggagaa ccggcaggcc cgcatcgagt gggcctggca ggtgtacgag cggaggcttg
     5821 ggctcctggg aaccctcgac gatgacgggc tgccgttcct ggatccctac accggcacgg
     5881 agatcttcgc gcaggcgttc ggctgccggg tggtatatcc cctggacaat atgcccttcg
     5941 ccctcccgct gatcgacaaa cccatggagg tctcgagggt ccaggtaccg gatctcgatt
     6001 ccccttcgct cgcgctcctc ttcgacattg ccgatgagct tcgtcgccga gcgggcccgg
     6061 gcgccctcat gcgcctgatc gatgtccaga gccccatgga catagcggcc ctgatctgga
     6121 acaagaacac gttctacgcc gccatgctgg acgcgcccga ggcggtgatc gagctggcgc
     6181 acaaggtgaa gaagctcctg gtcctgttcc tggatgcatg gttctcgcgg tacggcagct
     6241 cgttcatcgc ccactacccc gactactaca tgccggcggg cctgacgctc tccgaggacg
     6301 aagtcggggc cgtgagcccg cagatgttcg aacgattctt cctgcccgag ctcgccgagc
     6361 tatcgaaccg ctacggcgcg ttggggatgc actgttgcgc gagcgcccgc caccagtggc
     6421 aggggttcct caagatcccg aacctcaagg tgctgaacat ctgccagccg gccggcgtcg
     6481 tggcgcaggc gcaccggttc ttcgccgcca tctgcgctca gcagcacctc ccgccggagt
     6541 cctgggatgt gagccaggtg accccgtccc agtttccgtc cggcgcccac gtcgtgctca
     6601 ccagcgccgt gaagagccag gaggacgccc tccgctgctc ggcggcgttc caccgagcct
     6661 tccggtagcg tgccgcaggg tcggccttcc gcacgtcctg tgctcggccg acctgcgcca
     6721 tccctggcta cggataaatc ctcgagatgg tccgcgggaa cgggatgcac tcgcggatgt
     6781 gctggctgcc gcagatccag gcgagcgtgc gctccagtcc gatcccgaat cccgagtggg
     6841 gcaccgagcc gaacttccgc aggtccaggt accacccgta cgcgtcctcg ggcagcttct
     6901 cctcgtggat ccgggcaagg agcttctcca gcacgtcctc gcgctgcgag ccgccgatga
     6961 tctcgccgta gccctccggc gccagcaggt cggagcagag caccgtatcg gggttggcgg
     7021 ggttctcctt catatagaag gctttcgcct tcttgggata gttcatgacg aagatcggct
     7081 tctcgtgcat cttcgtcagg atcgtctcat cctcaccgcc caggtcgtct ccccattgga
     7141 tcgagctccc cttggactgc agcgtcttga ccgcatcccc atattccatt cgcacgaagg
     7201 gcggggtcac cttggtcagc gggacggtgt cccgctccag cgccgtcagg tccgccgcgc
     7261 agttggccag cacgtcggtc acgacgcggg tcaccatgtc ttcctggagc cgcatgttgt
     7321 ccgcgctatc ggcgtaagca acctcggcat ccagcatcca gaactcggtg aggtgacgcc
     7381 gggtcttgga cttttccgca cggaaggtcg gcccgaagtc gtagaccttt ccgtacgcca
     7441 tgcaggcggc ctccaggtag agctgcccgg tctgggccag gtaggcgtct cccaggtcga
     7501 agtagggcac cgtgaagagc gtcccggcgc tctccccgat ggacccggtc aggatcgggg
     7561 tgtcgatgag gagaaagccg cgctcgtgga agaagtcccg aaaggagcgg atggcctggt
     7621 cgcggatccg catgatggcc gcgggccgcg tggagcgaag ccagaggtga cggttctcca
     7681 ggaggaagtc gatgccgtgt tccttcttgg cgatggggta gtcctccgac gggttttgca
     7741 ccacctccag gccgctcagg gtgagctcga agccgctggg cgcgcgggag tcctggcgca
     7801 ccgtgccccg gacgatcacc gacgcctcca tgcgcagcgc cgagatggag gcgaaggcag
     7861 cggcgccgca ggcggtctcc tccgcgacgg cctggatccg acccgttccg tcccgaagct
     7921 gcaggaacgc gatctttccc ttggagcgca ggttgtagag ccagccgcgg atctccacct
     7981 cctggtccac gtgcttgccg atctccgcga tcgtcaccct gtccatgcga ttctcctctc
     8041 ggcccgcagg ccgtgcgctc tggtgcgtgc tggcgcgcga cggatccgcg cggctagaca
     8101 aacttctccc gctcgcgaag gatggcgatc tcgcccttct gttcgagcag ctggatgtac
     8161 tcgaggaaat cctggatggc gcggtccacg tccgcgcgcc gcgccggacc catggcatca
     8221 ctctccagct tcaccaggtc gcggcggtgc tcggagatgg agctggtgat ccgcgcgccg
     8281 acctcgtcgg agacgccctt gagcaccatc gcgatctccc ggtcggcgta gtcccgcagg
     8341 atcttctgca ggtccttgtc cgggatctgg aagacgacct cgatggtgta gagcattttc
     8401 cggatctggt tcgcggtgtt gggatcgagg tcggagagga tggcctgctc ccgcgtgggg
     8461 tccatgagcc gcaggatggc ggtgagggtc tgcgtgccgt cgacctcctg cgtcgccggc
     8521 tcccccaggt cccggacctt gctcttcagc gcttcctcgg ctcgacgcag gacctccggg
     8581 ctgacctgct gcatcttggc gatgcggccc accacctccc gctgcacctc cgggctcaac
     8641 gcctggagga tccgagcggc cagcttgggc tccaggtgcg ccaggatcag cgcggccacg
     8701 ggggcggatt cctggcggac cagggcgatg acctggtgga catccacgtc cttgaggaac
     8761 gtgaacggcg ggggcgccat gtcctttcgg accttctcga tgatctcccg cgccttctcg
     8821 gctcccaccg aggcgaccag catctcctgg gccttgtcga tcccgccctg cgcgatcaac
     8881 gcccgggtct ggctgaggta gccgaactcc tccaggacct tcgtggcgtc ccgctcatcg
     8941 atcttctggg tccgcgcgat ctctcgggtg atcccttcca cctcctgggg cgcgaggtgg
     9001 cgcagcacgc gcgcggcctc ctccttgccg aggagcatga ggaactgggc cgccttccgg
     9061 tagccggcgt ctcgactggt ctcccggatg aaccccatga cggagggatc cgatcgtggc
     9121 ggcttgggac gcggctcgcc gggctggcgg gccggttggc cggcgggacg cttggcgccc
     9181 ggtgcgcccg gcttctcagg agggttcccg ctcttggcca tgagccatac taccggtggg
     9241 caatcgtcag gtcaattcgg gaaaggagcg agagcgcttg gcttgccgcg gagcgcgcgg
     9301 tatcttggct tcgagcaaga tggccgagac gatcgctctc gtggcaaatg tagccctggc
     9361 tctctccctg gtcgtcggcc tggtgtttgg catcgcccag gtccgcgcgg cggcccgaga
     9421 ccggcgggag cgccttaccc tggaggccct gcgcggtttc cagagccgcg atttcgtcga
     9481 gctgatgcag tacatcatcg ctcacgatat ccccaagagc cgcgcggaga tgagcgctct
     9541 ccccgacagg gaacaggtcg tcttcctgca gtgctcccag gcgatggaga tgctgggcct
     9601 cctggtcgcc gagcggttga tcgacctcga cctggtcgag aggaccctcg gatccttcgt
     9661 caccacatcc tgggagaagc tcaagtccat cacggtcgac gttcgacaga ccgacccgtt
     9721 cctgcacgag tacttccagt ggctggcgga acggatcggc gagcggatgt ctcgacatcc
     9781 acgtcgaccg ttccacgaaa cggggccgag gccgacctga catggcatcg gaccggtcgg
     9841 tcgtcgaacg cgtcgaggag ctctaccgcg cagaatccac ccgggtgttg gcgaccctga
     9901 tccgcctgct gggggacttc gatctggcgg aagagtccct ccaggaggcg ttccgcgcgg
     9961 ccctggagca gtggccgaag gagggcctgc cgcgcaatcc gcgcgcgtgg ctggtctcta
    10021 ccggcaggtt caagggcatc gacgtggtcc gccgcagggg gcgtcacgac gccctgctgg
    10081 ccgcggttgc ccaggagagc cgggaagcct tcgtggatcc agaggccgtg gacgacgaga
    10141 cgcttcagga cgaccagctc cgcctcattt tcacctgctg ccatccttcc cttcctcaag
    10201 agggccggat cgccctggcg cttcgggagg tctgcggcct cacgacggag gagatagcgc
    10261 gcgccttcct cgtcaccacg gcgaccatga cccggcggat tgcccgcgcc aaggaaaaga
    10321 tccgccagga gcgcatcccg tacgcggtcc cctcccgcca ggagctcgcg cggcggcttg
    10381 cgaccgtcct gcaggtcgtg tacctggtct acaacgaggg tttcgtcagc acggcaggcc
    10441 aggctctctc ccgtcgggac ctggcggagg acgcccttta cctcggccgg ctgatcgtgg
    10501 agctcattcc ggagccagag gccatcggcc tgttgggcct gatcctcctg cacgaatccc
    10561 gaaggccggc gcggaccgac gcgaacggcg acgtgatccc ccttgaggac caggaccggt
    10621 cgctctggaa ccgggagcag atcgaggaag ggatcggact ggcccatcgg gccctgctgt
    10681 ccggccggtt cggctcctac acgcttcagg ccaccatcgc cgcggttcac gccgtggcgc
    10741 cgtccgtggt ggagacggat tgggaccaga tcgtccgcct ctacgacatg ctcctggaaa
    10801 cgggtccctc cccggtcgtg gagctcaatc gggccatcgc cgtggcgatg cgagacggcc
    10861 ccagcgccgg gttgtccctc atcgacgcgc tgctggagcg gggagagctc gcggactacc
    10921 accccgtgca tgccgtgcgc gcggacctga accggaggct ggggcgtgtc ggcgaggcga
    10981 cggcgtccta ccgacgcgcc ctcacgctgg cacgtcagga gcctgagcgg cgctacctgg
    11041 aaaagcggct cgcggaagtc tcgggatcat cggcgagctt gtccgttttg gcggtccccg
    11101 tccgactact agacgagatt tcatgaggta aggcgagggc ctggcgggac cgccggggcg
    11161 gacgccttgc cggaaggagc aggtatcatg aatgcgcacg tgagctcgat tctcctgggt
    11221 gtccgggaca tggagcgatc caagcggttc tatacggagg gtctcggctg gaaggtccag
    11281 aacgactacc gcatctccgt gttcttcgtt ccgcacgggg gatccatcgt cggcttctat
    11341 ggccgtgacg gtctggccgc atccgtgggc gcgagccccg agggcagcgg cttcagcggc
    11401 gtggtcttca actacgtggt ccgaagcgag gcgcgggtcg acgagatcct atcggaggcc
    11461 cggaaggccg gcgcaacgat tctcaagccc gccgccgccc tgccgtgggg cgggtatggc
    11521 ggctctttcg cggatccgga tggatacatc tggaacgtcg gctacagcgc ccagggaaag
    11581 gaccagccct acgcggagta ggcgcggttg aggaaggaga gggaaatgca gttcttcgtc
    11641 tatggttccc catcggagag gcagcagcct cgggaccccg ccgagctggg gaagttcatg
    11701 gaggagtccg tcaagaaggg aatcctcatc acggggggcg ggctccacaa gaaagagacc
    11761 cgggtcgtcc ttgccgaagg caaggtctcg atcaccgacg ggccctttct cgagggcaag
    11821 gagttgatcc ctggcttcac ggtcatccag gtggacacca aagagcaggc catcgagtgg
    11881 gtccgcagcc ttcgacagct ctacggggac ggggagacga agctcgtgcc gatcttcggg
    11941 ccgtcgtagg gctttacagg ccgcgccgac ggtcctcgtg gtcagacggt cgtggagccc
    12001 ggcgggaggg aaggatggcg caggaataca tcgagatcag gggcgctcgg gaaaacaacc
    12061 tgaagaatgt ttccctgaga attcccaaga gaaagatcac gctctttacg ggtgtgtccg
    12121 gctccggcaa gtcctccctg gtctttgata ccat
//
LOCUS       DASRZS010000385         3184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5598796, whole genome shotgun sequence.
ACCESSION   DASRZS010000385 DASRZS010000000
VERSION     DASRZS010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5598796"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VFH83_09410"
     CDS             <1..941
                     /locus_tag="VFH83_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6486624.1"
                     /translation="DPSVPDAYPVLEREYHTPEGVLLHSVRQTGEQQGEGWVVQPPRV
                     PLIEDFNIPRAVRQVVTEPQDVARLSFCYRPPDAAARQWFSRRMSEVGPFRQREGFPV
                     QAWAGFGMDAVVWFCGTEGAVLFAMDHPREFQELFDIITETDLGRVELAAADPGVDLV
                     VERGWYSSTNFWSPALLDRHLFPHVRSLAGAAHRHGKKFAYVMTTGVEVLGPRLAEAG
                     VDVLYFVDPLDPVQKGLSLEKVRGLLGGAMTLVGGISSITLGNGDAQAIDRAVRHALD
                     VLGPTGRFILHPVDALFPDTPWKSVECLIESWRNAR"
     gene            complement(965..2182)
                     /locus_tag="VFH83_09415"
     CDS             complement(965..2182)
                     /locus_tag="VFH83_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET6486625.1"
                     /translation="MDQRNSSRRDFRVMQAIYFLPTPTRNEIARFTGRSPVSVTETLK
                     RLMEAGLVEKAGKTRSRSGRPSMTYQLGEKTGCSVGVSLDPSGFRVVALDSRHAVVQE
                     VEKTLTLSANPVSHLEEIMEQLTGQLRRFLAAMELKHQRTLSIGIAPPGMVDTEKGVW
                     LHGLQVSGIAHVSLGATLQKMFSAPVVVEDTARCLAFLEASRRTPELARDLVYLYLAS
                     GVGAGIMINGEPYHGGHGMAGEVGHLAVEEEGARCSCGNIGCLETVISGASILQRFRR
                     RLDEGVISSLQEHRGGLTLEVIRQAAEAGDRLAASTLFELGTLLGDAVSKIIKLYNPR
                     TLVIGGPVAVLAEHLRESMWLKVRQKVIPEMLVNLALQVAPSQPRDEAIGAAYLAERK
                     FWKEGDERSLAAS"
     gene            2384..>3184
                     /locus_tag="VFH83_09420"
     CDS             2384..>3184
                     /locus_tag="VFH83_09420"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6486626.1"
                     /translation="MPGPGFEWIGKEEEKEVLEVLRDRWLFRYGDEKDPAFKRKVKTL
                     EELVEQRFGSRHALAVTSGTAALITALSALGIGPGDEVIVPGYTFIASMSSIIVARAV
                     PVLAEIDESLTLDPEDVEKKITPRTKAIMAVHMLGNPCNIDALQAIAHKHKLILIEDA
                     AQAFGATYKGRRVGTIGKIGTYSFNIFKTINAGDGGMVVTDDDDLYFRAFGYHDQGHF
                     PSRSGVEIGNRSIIGQNYRMNEITGAVLIAQFRRLDDIIARLKQIKTRF"
BASE COUNT          566 a         1031 c         1034 g          553 t
ORIGIN      
        1 gcgacccctc tgtccctgat gcctacccgg ttctcgaacg ggaataccac acgccggagg
       61 gcgtgttgct ccattccgtg cgccagacgg gcgagcaaca gggagagggc tgggtggtcc
      121 agccgccgag ggtgccgctg atcgaggact tcaacatccc ccgggcggtc cggcaggtgg
      181 tcacggagcc gcaggacgtg gcacggctgt ccttctgcta ccggccgccg gatgccgcgg
      241 cccgccagtg gttctcgcgg cgcatgtcgg aggtgggtcc gttccgccag cgcgagggat
      301 tccccgtcca ggcgtgggcc ggcttcggga tggacgcggt ggtgtggttc tgcgggaccg
      361 agggcgcggt tctctttgca atggaccatc cccgcgagtt ccaggagctg ttcgacatca
      421 tcacggagac cgacctcggc cgcgtagagc tcgccgccgc ggacccgggc gtggacctcg
      481 tggtcgaacg gggctggtat tcctccacga acttctggtc gcccgcgctc ctggaccgcc
      541 acctctttcc ccacgttcgc agcctggccg gcgcggcgca ccggcacggg aagaaattcg
      601 cctacgtcat gacgaccggc gtggaggttc tcggcccccg gctggcggaa gccggcgtgg
      661 acgtcctgta cttcgtggac ccgctggatc ccgtacagaa ggggctttcc ctggagaagg
      721 tccgcggcct gctgggcggt gcaatgacgc tggtcggagg catcagctcc atcacgctgg
      781 gaaacgggga tgcgcaggcc atcgaccggg ccgttcggca cgcgctggac gtgctgggac
      841 cgaccgggcg gttcatcctt cacccggtgg acgcgctgtt ccccgacacg ccgtggaagt
      901 ccgtggaatg cctgatcgag agctggagga acgcccggta gccgaagccc ccgcgtgcgg
      961 ggggtcagga agcggcgaga ctgcgctcgt cgccttcttt ccagaacttt ctctccgcga
     1021 ggtacgcggc gccgatggcc tcgtcccgcg gctgggaggg cgcgacctgg agcgccaggt
     1081 tcaccagcat ctccgggatc actttctgac ggaccttgag ccacatcgac tcgcgcaggt
     1141 gctcggcgag gaccgccacc ggtcccccga tgacgagcgt ccgagggttg tacagcttga
     1201 tgatcttgct gacggcgtcc cccaggaggg tgcccagctc gaacagcgtg gaggcggcca
     1261 ggcggtcacc cgcctcggcg gcctggcgga tcacctccag ggtcaggccg ccgcggtgct
     1321 cctggaggga ggagatcacc ccctcgtcca gccgccgccg gaaccgctgc aggatgcttg
     1381 cacccgagat cacggtctcc aggcagccga tgttcccgca ggaacaccgg gccccttcct
     1441 cttccaccgc caggtggccc acttccccgg ccatgccgtg tccgccgtgg tagggctccc
     1501 cattgatcat gattcccgcg ccgacgccgg acgcaaggta gaggtagacc aggtcacgcg
     1561 ccagctcggg cgtacgccgg gatgcctcga ggaacgcgag gcagcgcgcc gtatcctcca
     1621 cgaccaccgg cgcggagaac atcttctgca gcgtggcgcc gagggagacg tgggcaatgc
     1681 ccgacacctg cagcccgtgc agccacacac ccttctcggt gtccaccatg ccgggcgggg
     1741 cgatgccgat ggacagggtc cgctggtgct tcagctccat ggcggccagg aacctccgaa
     1801 gctgcccggt gagctgttcc atgatctctt cgaggtggga gacggggttc gcggagagcg
     1861 tgagcgtctt ctctacttcc tggaccacgg cgtgccgcga gtccagcgcc acgacgcgga
     1921 agcccgaagg gtcgagggag acgccgaccg agcagccggt cttttctccg agctggtacg
     1981 tcatggacgg ccgcccgctg cggctgcggg tcttccccgc cttttccacc aggccagcct
     2041 ccatgagcct cttcagggtt tccgtgacgg aaaccgggct cctcccggtg aaccgcgcga
     2101 tctcgtttcg cgtcggcgtg ggaaggaaat agatagcctg catgacgcgg aaatcgcggc
     2161 gcgagctgtt cctctggtcc atatttacgc gaaagtttaa cgtaatgcaa aacattgttc
     2221 aagaacaaag ttttatacaa ttgtcgatct ttgaagggct tatatagaaa cagccgaccg
     2281 accgtggatc tctcgttatg ttacagattc cataaattgt tgacaaaaca gcctttcatg
     2341 gtattatttc aagcacaatt tcacataaac aggaggcctc tagatgcccg gacccggctt
     2401 tgagtggatt ggcaaggaag aagaaaaaga agttctggaa gtgctcagag accgctggtt
     2461 gttcaggtac ggggacgaga aggatcctgc gttcaaacgg aaggtgaaga cgctggaaga
     2521 gctggtggag cagcggttcg gctcccggca cgccctggcc gtgacctcgg ggacggcggc
     2581 gctcatcacg gccctttccg cgctgggcat cggccccggt gacgaggtca tcgttcccgg
     2641 ttacacgttc atcgcttcca tgtcctccat catcgtggcg cgcgcggtgc cggtgctggc
     2701 cgagatcgac gagagcctca cgctggaccc ggaggacgtg gagaagaaga tcaccccgcg
     2761 caccaaggcg atcatggccg tccacatgct cggcaacccg tgcaacatcg acgccctgca
     2821 ggccatcgcg cacaagcaca agctcatcct catcgaggac gcggcacagg catttggggc
     2881 aacctacaag ggccggcgcg tcgggaccat cgggaagatc ggcacctact cgttcaacat
     2941 cttcaagacc atcaacgccg gagacggcgg catggtggtc acggatgatg acgacctgta
     3001 cttccgggcc ttcggctacc acgaccaggg ccacttcccc agccgctcgg gcgtggagat
     3061 cggaaaccgg tccatcatcg ggcagaacta ccgcatgaac gagatcaccg gggctgtcct
     3121 catcgcccag ttccgcaggc tggacgacat catcgcgcgc ctgaaacaga tcaagacccg
     3181 gttc
//
LOCUS       DASRZS010000386         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2521871, whole genome shotgun sequence.
ACCESSION   DASRZS010000386 DASRZS010000000
VERSION     DASRZS010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2521871"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFH83_09425"
     CDS             complement(<1..511)
                     /locus_tag="VFH83_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486627.1"
                     /translation="MISLPKIIRMGAIVLALYGLAGCMTAASTPAAFAPDSVVFLAAA
                     AIPGGADASADKSGLPHSLLEDGQSYQDSFDGMGSAFAYTGFGTRYVALADRGPNKVQ
                     YPGGDVVDFTTSYRNRFQIVDVAVTRASGDWTVVPRLYGTSLLVDDVGRGYTGISTAF
                     TGADPTANMR"
     gene            complement(838..2181)
                     /locus_tag="VFH83_09430"
     CDS             complement(838..2181)
                     /locus_tag="VFH83_09430"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6486628.1"
                     /translation="MPSHTSRTVLAAAFVTVLAAGSAGLWAEPAPASLTVEEAVAAAL
                     RNNLTVRAAATEERARKAESDLSFNRFYPTVSVSGSVVQLNQPSTVLVPTPTGGVFPY
                     TPSPTNLALNMSIQEVFSPTYLVLIKEDALSYRDSSIDRTQAQRQITAAVKKTFYQLQ
                     AQKETIALTRSRLEKAQERLRQAQVSYQLGQSSELNYVYANENVQGIMPALRSLETSY
                     QAMLTQFEQILGFDTRLDMELAGSLDVGDVTPDRWTGRESERLDVRKAAIGVEQIETG
                     LNAESTVLLPTIILQYKADPALNNPASTSIWQGSNWMQSMGGVYLTLSWDLSPLLPGS
                     DYWVKLGRYNDQLALARATAADTMRKARDDTEDQKRAISASIANIDNLQRSLESARRA
                     YDLTDISYRAGAGRLLDLQDAELAWQSAQVQLLSEKLKLASLAYDWDAKYAPAPQ"
     gene            complement(2174..>2721)
                     /locus_tag="VFH83_09435"
     CDS             complement(2174..>2721)
                     /locus_tag="VFH83_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6486629.1"
                     /translation="PLAVMFCLPFAMVGGFASLAISRQTLSIFSIMAIILLIGLAAKN
                     AILLVDRALKNRDERQMDLVEAFKEAVATRIRPIFMTTAAMVFGMLPVAMGLGSAGEM
                     KSAMGVVLIGGLVFSMVVTMIIVPVAFLSVDSVRSRVLRPRGARDTEGRSNGTSRNGS
                     GNNGTWGAAMPSDRRREKINA"
BASE COUNT          451 a          856 c          911 g          503 t
ORIGIN      
        1 gtcgcatgtt ggcggtcgga tccgccccgg tgaaggccgt gctgattccc gtgtaacccc
       61 gtcccacgtc gtcgaccagc agtgacgtcc catagagacg gggcacgacc gtccagtcac
      121 ctgatgccct ggttaccgca acatccacga tctggaagcg attccggtag ctggtggtga
      181 agtccacgac gtcgccgccg ggatactgga ccttgttcgg cccgcgatct gccagggcga
      241 cgtaacgggt tccgaagccc gtgtaggcga aagcggaccc cattccgtcg aagctgtcct
      301 ggtagctttg gccgtcttcc aggagggaat gcggcagccc ggacttgtca gcggaagcat
      361 ccgctccccc ggggatggcc gcggccgcga gaaagaccac ggagtccggt gcaaacgccg
      421 ccggcgtcga tgcggcggtc atacagcctg ccagtccata gagagcgagc acgatggctc
      481 ccatccggat gatcttcggg agagaaatca ttgcggctcc tttccgtaac ggctgcaata
      541 agctctctcg tctctcttag ggttctgttg cttttctgtc agaattgtgg gaagaaatgc
      601 cttgccgcgg acttcatgcg ctcacggcca ttgccgcaca tgccccgcac cttgcggggc
      661 atgatggagg cgaacgcggg tggtgtcaag aagaacgcaa ggtcggcgaa cgggcgcccg
      721 ggaaggaacg ccagcggcgg caccttggac gggcccggtg acgccactcg cgtcaccggg
      781 tcggatgcca ccgcgccgca aggcacccgt ggtctggcaa cggcggaagc cgttcggtca
      841 ctgcggtgcc ggcgcgtact tggcgtccca gtcgtatgcc aggctggcga gcttcagctt
      901 ctcgctcaga agctgcacct gggcgctctg ccacgcaagc tcggcgtcct ggaggtccag
      961 gagccgtccg gcgccggcgc ggtaggagat gtccgtcaga tcgtaggcgc gtcgcgcgga
     1021 ctccagcgaa cgctgaaggt tgtcgatgtt ggcgatgctc gcgctgatgg cgcgcttctg
     1081 gtcctcggtg tcgtcccgcg cctttcgcat cgtgtccgcg gccgtggcgc gcgcaagggc
     1141 aagctggtcg ttgtagcggc ccagcttcac ccagtagtcg gaacccggca gcagcggtga
     1201 caggtcccag gaaagggtca ggtagacgcc tcccatgctc tgcatccagt tgctgccctg
     1261 ccagatgctg gtggaagcgg ggttgttgag cgcgggatcc gccttgtact ggagaatgat
     1321 ggtgggaagg aggacggtgc tctccgcgtt cagaccggtt tcgatctgct ccacgcctat
     1381 ggctgccttg cggacgtcca ggcgctccga ctcccgtccg gtccagcggt ccggcgtcac
     1441 gtctccgacg tcgaggctgc cggcaagctc catgtccagg cgcgtgtcaa agccgagaat
     1501 ctgctcgaac tgggtcagca tcgcctggta gctcgtctcc aggctccgca gcgcgggcat
     1561 gatgccctgc acattctcgt tggcgtagac gtagttgagc tccgatgact ggccgagctg
     1621 gtaggagacc tgcgcctgcc gcagtcgttc ctgcgctttc tccagccggg aacgcgtgag
     1681 ggcaatcgtt tccttctgcg cctggagctg gtagaaggtc tttttcaccg ccgccgtgat
     1741 ctggcgctgt gcctgtgtcc ggtcaatgga gctgtcacga tagctcagcg cgtcctcctt
     1801 gatgaggacg agatacgtcg ggctgaacac ctcctggatg ctcatgttca gggcaagatt
     1861 cgtggggctg ggggtgtagg ggaacacgcc cccggtgggc gtgggaacga gcacggtact
     1921 gggctggttg agctggacga ccgacccgct cacggagacg gtgggataga acctgttgaa
     1981 cgagagatcg ctctccgcct tccgggccct ttcctccgtg gccgccgccc tcaccgtgag
     2041 gttgttccgg agggccgctg ccacggcctc ctccacggtg agcgacgccg gcgccggctc
     2101 ggcccagagg ccggcgctgc ccgcagcgag caccgtcacg aaggcagcgg ccagaacggt
     2161 acgcgatgtg tgactaggca ttgatcttct cccgtcggcg gtcggagggc atggcggcgc
     2221 cccacgtgcc gttgtttccc gaaccgttgc gcgacgtgcc gttgctccgt ccctcagtgt
     2281 cccgtgcgcc ccgaggccgc aggacgcggc tgcgcacgct gtcgacggac aggaatgcca
     2341 ccggcacgat gatcatcgtg accaccatgc tgaagacgag gccgccgatg agcaccactc
     2401 ccatggcgct tttcatttct ccggcggagc ccagtcccat ggccacggga agcatgccga
     2461 acaccatggc ggccgttgtc atgaagatgg gccggatgcg cgttgccacc gcttccttga
     2521 acgcttccac gaggtccatc tgccgctcgt cgcggttctt caacgcccgg tccaccagga
     2581 ggatggcgtt cttggccgcg agcccgatga gcaggatgat ggccatgatg gaaaagatgc
     2641 tgagcgtctg ccgcgagatg gcgagggacg caaagccgcc caccatggca aagggaaggc
     2701 agaacatgac ggcaagggga t
//
LOCUS       DASRZS010000387         7565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2132277, whole genome shotgun sequence.
ACCESSION   DASRZS010000387 DASRZS010000000
VERSION     DASRZS010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2132277"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1045
                     /locus_tag="VFH83_09440"
     CDS             200..1045
                     /locus_tag="VFH83_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6486630.1"
                     /translation="MRLKRYEIPGEIARNAVLLVASFLFLIPFYVAIVNVFKTRLDIV
                     RHPMSIPFGRLTLDNILRNLFAPHFNVLIAYGTSLVIAGLTVGFVVLFASMMSFVLCR
                     KKHAFFKYAYLLLLAGLMIPAQVILLPIVQVLRRLHLMFTVQGLLLMNIGWYMPFAAF
                     LFVGYMRTVSPQLDESARMDGASDLRIFFRIIFPLISPCVASVIIFVALWTWNDFVNP
                     LIILGTSQFYTVTIGVYRAIGQYVQKWEDVFAVVFIAIVPVIIFYLFMQKQFVAGLTA
                     GALKG"
     gene            1149..2345
                     /locus_tag="VFH83_09445"
     CDS             1149..2345
                     /locus_tag="VFH83_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6486631.1"
                     /translation="MQFRHMKKIDQKVSALGFGVMRLAGRGGMMRLRVDEKRAITAIR
                     RGIELGINYFDTAYIYHLGASERILGQALRDGYRQKVLVADKLPMILMRRPEDFSRFL
                     ETQLKRLGTDHIDFYLFHSMNRAYLKKMRDFDLLPKMEDARRRGLIRYIGFSFHDTLP
                     IFKEVIDAYAWDMTQIQYNYLDTGVQATTEGLKYAYAKGIPVVVMEGLRGGKLANPPM
                     EALQAMRDGSSSRTPVDWAFQFLWNQEEVTTVLSGMNSAAMVEENCRYADRSGVGTLT
                     GKDQEVIRKLVDVYRQKILVGCTACNYCMPCPHGVNIPESFAQLNMSNGPRGLQSWLI
                     RRNYGRLASRRDRVNRAVPNGNASLCTQCKVCVPQCPQGIDIPTELAKVDLVLGKRRR
                     IEEVFG"
     gene            complement(2379..3512)
                     /locus_tag="VFH83_09450"
     CDS             complement(2379..3512)
                     /locus_tag="VFH83_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6486632.1"
                     /translation="MSAAFTPKQRMLAAYRGQPCDRPPVAPEFWYYYPARLLGVDMIA
                     FERDVPFWLALKRSFERWSCEGWGAAFADLRIGGMSLRRSETAIGEGRYRETTVQAFA
                     GHRFESTKIYDKREPSAMERYPVTDPEDLSAYLAMALSPEFELDFSAAIRGHTAVGET
                     YLLEYWLGVPFFDWIAEATGFEGAVVWVTSAPQREVEELRERYQTRIIEVLRLAAART
                     PFESFAVGCSSSCNSLLGPTLWRKLDKPFLTAVTREAHRLGKLLHMHFHGRSRQALED
                     FVQIGADCVCPFERPPGGDIEGAAGLRAARSALAEAVTFNGNVHTVETLIRGTPEDAR
                     REVREIKGAFQGSRRFIIGTGDQVGGETPDENIAALIEEGKAD"
     gene            3622..4227
                     /locus_tag="VFH83_09455"
     CDS             3622..4227
                     /locus_tag="VFH83_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET6486633.1"
                     /translation="MSYIEPPRRISLVYRLMLGLVERRLGKRLEANRILTWYPKALIG
                     SGLMEALVAHHERDVPRRLLTLVRVYTSFRVSCPFCIDLNSREFQQKGISDEEILALQ
                     AAVRLGSSDSLDSVASLSPRERAALRYAECICKTPLEFSEAVLADMKRHFSERGIVIV
                     ASTCAQVNFWARLIQSLGVPPAGFSAECSILNLETYRTLRR"
     gene            complement(4361..>7565)
                     /locus_tag="VFH83_09460"
     CDS             complement(4361..>7565)
                     /locus_tag="VFH83_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6486634.1"
                     /translation="GGLGVEVLSAETIREYRRLAAEQPPDIALVDLNLPDGRATELLT
                     PPPRFGPFPVLVMTSFGNETTAVEALKAGALDYVVKSPDAFGALPRTVTRALREWNLL
                     QEREQTQEQLRGSEERFRALVQNAADAVTLVDAHGVVLYTTPAVTRMLGYTPQAIVGR
                     NLRDVMHPEEVESMAVDYRGLVAEPGGTRTFLLRLRHEDGSWRWAECVTSNMLDHPAV
                     RAIVVNYRDVTERKNDERALREQEEHYRRLFDESLEGLAVADATTGILLDCNQAFQRL
                     TGYSREELIGAPQVKIHPSEEGYARLSRAFVQHRGEQAGQVIEELILTKSGAIREAEV
                     KAMPIEIAGRKVLLGTFHDVTEIRRGRREREVTLALLQLLNDRGHLHELIRGVLTIVQ
                     RWTGCAAVGLRLREGEDFPYLEVQGFPAAFVERENHLCEHGADGRPSRDEEGMPQLAC
                     LCGDVLRGSFDPALSCFTDKGSFWTNALASTRFADTARPGGYRSRCLATGYQSMALIP
                     LRHGRQVLGLLQLNDPVAGRFSIELVSFLEQLADQIAITIAQRQAELAMRASEERYRR
                     IVETTLEGIWTVDPEGRATFVNQRLAAMLGYAPQEMIGVPLEHFVVPEDREAWRQRMD
                     RARRGEALPGHETRYRRKDGADLWAIVSTSPIPGPDNRLAGSLGIVSDITERKKAEDD
                     HARLQAQLNQSQKMEAIGLLAGGVAHDFNNMLTVILGYSDVLLGRLRPEDPNRAYVAD
                     IRDSGQRAATLTRQLLAFSRKQILTPEIVDLNEVVSTIEKMLRRLIGEDIVLTTIYTP
                     LLPRVRVDPGQMEQVIINLAVNARDAMPQGGRLIIETSGADLDEEFCRLRPGSRPGRF
                     ALVSVTDTGTGMSPEVKSRVFEPFFTTKEYGKGTGLGLATVFGIIKQSEGYIDFLSEV
                     GAGTSFRIYIPAVEAEDSAALSVIEAGAPPGHETVLLVEDEEGVRQVARLALESFGYK
                     VIEAANSRDALSAAELAMDPIDLLLTDIVMPGMSGRQLAESLRKRYPGLKVLYMSGYT
                     DDAVVRHGVTAASAPYLHKPFPPLDLARKVREVLDAK"
BASE COUNT         1214 a         2566 c         2442 g         1343 t
ORIGIN      
        1 ggcgccaccc tgaccatcgc ctaccaggtc atcttcgtag ggttcacccg aaacatgttc
       61 tcctacggga ccagcctggc catctacatg ctggccatcg tcgccgtgtt ttccgtgacg
      121 atgacctact acctgaggag acgggaagag aggctgatcc tgtgaaggct gaccctggac
      181 tccggaggag ccatccgcca tgcggctgaa gcgatacgag atccccggcg agatcgcccg
      241 aaacgcggtg ctcctggtgg cctcgttcct gttcctgatc ccgttctacg tggccatcgt
      301 caacgtgttc aagacgcggc tggacatcgt ccgccacccc atgtccatcc ccttcggcag
      361 gctcacgctg gacaacatcc tgcgcaacct ctttgcgccg cacttcaacg tcctcatagc
      421 gtacgggacg tccctggtca tcgccggcct caccgtcgga tttgtcgtgc tgtttgccag
      481 catgatgtcc tttgtgctgt gccgcaagaa gcacgccttc ttcaagtacg cgtacctgct
      541 cctgttggcc ggcctcatga ttccggctca ggtcatcctc ctccccatcg tgcaggtcct
      601 gcgccggctc cacctcatgt tcaccgtgca ggggctgctg ctgatgaaca tcggctggta
      661 catgcccttc gccgcctttc tcttcgtggg ctacatgcgc accgtctctc ctcagctcga
      721 cgagtccgcc cggatggacg gagccagcga ccttcggatc ttcttccgca tcatctttcc
      781 gctcatcagc ccttgcgtgg ccagcgtcat catcttcgtg gcgctgtgga cgtggaacga
      841 cttcgtgaac ccgctgatca tcctggggac gtcccagttc tacacggtga ccatcggcgt
      901 ctatcgggcc atcggccagt acgtgcagaa gtgggaggac gtgttcgcgg tggtgttcat
      961 cgccatcgtg ccggtgatca ttttctacct cttcatgcag aagcagttcg tggcggggct
     1021 tacggcgggg gcgctgaagg gataacggcc gacgcgggct cctccacccc gacccgccta
     1081 gtgactcgat tcggcagcgt gtctatactg cccgggcacc gcaccagttc tgcaacagga
     1141 gctcctctat gcaattccgg cacatgaaga agatcgacca gaaggtgtct gcccttggct
     1201 ttggcgtcat gcggctggcc gggcgcggcg gcatgatgcg cctgagggtc gatgagaaga
     1261 gggccatcac ggcgatccgc cgcgggatcg agctggggat caactacttc gatacggcgt
     1321 acatctacca cctcggcgcc agtgagcgca tccttgggca agcgctgcgg gacggctacc
     1381 ggcagaaggt cctggtcgcc gacaagctgc ccatgatcct gatgcgccgg ccggaggatt
     1441 tcagccggtt cctggagacc cagctcaagc ggctggggac cgatcacatc gacttttacc
     1501 tcttccactc catgaaccgg gcctacctca agaagatgcg ggacttcgat ctcctaccca
     1561 agatggagga cgccaggcgg cgtggcctga tccggtacat cgggttctcc ttccacgaca
     1621 cgctgccgat tttcaaggaa gtcatcgacg catatgcctg ggacatgacg cagatccagt
     1681 acaactacct ggacaccggc gtccaggcca ccacggaggg gctgaagtac gcctacgcca
     1741 aagggatccc cgtcgtggtc atggagggcc tgcggggcgg caagctcgcc aatccgccca
     1801 tggaggcgct tcaggccatg cgcgacggat cctcctctcg caccccggtg gactgggctt
     1861 tccagttcct gtggaatcag gaggaggtta ccacggtgct gagcggcatg aactccgcgg
     1921 ccatggttga ggagaactgc cgctacgcgg accgctccgg cgtcggaacg ctcaccggca
     1981 aagaccagga ggtgatccgc aagctggtgg acgtctaccg ccagaagatc ctggtgggct
     2041 gcaccgcatg caactactgc atgccttgcc cccacggcgt caacatcccg gagagcttcg
     2101 cgcagctcaa catgtccaac ggaccgcggg gactgcagtc ctggctcatc cgccggaact
     2161 acggcaggct ggcctcgcgc cgcgaccggg tgaaccgcgc cgttcccaac ggcaacgctt
     2221 ccctgtgcac ccagtgcaag gtctgcgtgc cccaatgccc gcagggcatc gacatcccca
     2281 ccgagctcgc gaaggtcgac ctggtcctgg ggaaacggcg gaggatcgag gaggtcttcg
     2341 gataggccgc gttcttttcc ggtccggacc ggcggcattc agtcggcttt cccctcctcg
     2401 atcagcgccg cgatattctc atccggcgtt tctccgccga cctggtcccc cgtgccgatg
     2461 atgaaccgcc gcgatccttg gaaagccccc ttgatctccc gaacctcgcg ccgcgcatct
     2521 tccggggttc cgcggatgag ggtctccacc gtgtggacgt ttccgttgaa ggtcaccgct
     2581 tctgccagag ccgagcgcgc ggcgcgcagg ccggcggcgc cctcgatgtc cccgccgggc
     2641 ggccgctcga acggacatac gcagtccgcg ccgatctgga cgaagtcctc gagggcctgc
     2701 cgggagcggc cgtggaagtg catgtggagc agcttcccta gccggtgcgc ctcccgggtg
     2761 accgcggtca ggaagggctt gtcgagcttg cgccagagcg tgggccccag gagcgagttg
     2821 caggaggagg agcaccctac ggcaaaggac tcgaagggcg tgcgcgcggc ggccagccga
     2881 aggacctcga tgatccgcgt ctgataacgc tcgcgaagct cttccacctc gcgctgcggc
     2941 gccgaggtga cccagactac cgcgccctcg aaccccgtgg cctccgcgat ccagtcgaaa
     3001 aacgggaccc ccagccagta ctccaggaga taggtctcac cgacggcggt atggccccgg
     3061 atggccgcgg agaagtcgag ctcgaactcg ggagagagcg ccatggcaag ataggctgaa
     3121 agatcctcgg ggtccgtcac cgggtatcgc tccatcgcgg agggctcgcg cttgtcgtag
     3181 atcttcgtgg actcgaagcg gtgtcccgcg aaggcttgca ccgttgtctc gcgatagcgg
     3241 ccctccccga tggcggtctc ggatcgcctc aggctcatgc cgccgatgcg gagatccgca
     3301 aacgccgcgc cccaaccctc acaggaccat cgctcgaatg agcgcttcag ggccagccag
     3361 aacgggacgt cccgctcaaa ggcgatcatg tccaccccca gcagccgtgc cggatagtag
     3421 taccagaact ccggcgccac ggggggccga tcgcagggct ggccgcggta ggcggccagc
     3481 atccgctgtt tgggggtgaa tgccgcgctc atgtcggagg gatccgattc tcagccgtcg
     3541 ccgcctcgcg gtcaagggcg ctgagccgtc gcaggccgct gccccccttg actcccgtcc
     3601 cggatcgggc gaacaaggac catgagctac atcgaaccac cgcggaggat atccctggtc
     3661 taccggctga tgctgggtct ggtggagaga cggctgggga agagactcga agccaaccgc
     3721 atcctcacct ggtaccccaa ggcgctcatc ggctcaggac tgatggaggc gctggtggcg
     3781 caccacgaac gcgacgtccc gcggaggctc ctgacgctcg tccgggtcta cacctccttt
     3841 cgggtctcct gtccgttctg catcgacctg aactcccgcg agttccagca gaaggggatc
     3901 agcgatgagg agatcctggc gcttcaggct gccgtgcgcc ttggttcctc ggacagcctg
     3961 gactccgtgg ccagcctctc cccgcgggag cgagcggcgc tccgatacgc cgagtgcatc
     4021 tgcaagaccc ccctggagtt ctctgaggcc gtcctggcgg acatgaagcg ccacttctcg
     4081 gagcgaggga tcgtcatcgt ggcaagcacc tgcgcgcagg tgaacttctg ggcccggctc
     4141 atccagtccc tgggcgtacc gccggcgggc ttctccgcgg agtgctcgat cttgaacctg
     4201 gaaacctacc gcaccctgcg gaggtaggac gcatgggagt ccgcgtccgg tgtaaccctc
     4261 cgggccgacg ctcgtctggc tcggcttcgt tcacggagcg gtggccgtag ccggattctt
     4321 gtctctgccg gccttccagc tcacgggagg gtgagacagc ctacttcgcg tcgaggacct
     4381 cgcggacctt tcgcgccagg tccagcggag ggaacggctt gtggaggtac ggcgcgctcg
     4441 ccgccgtgac accgtggcgc accacggcgt cgtccgtgta tccgctcatg tagagcacct
     4501 tcaagcccgg gtaccgcttg cgcagggact cggcgagctg tcggccgctc atgcccggca
     4561 tgacgatatc cgtgagcagg aggtcgatcg gatccatggc cagctccgcg gcgctgagcg
     4621 cgtcccggct gttggctgcc tcgatgacct tgtagccgaa gctctccagg gccagtcggg
     4681 caacctggcg gactccctcc tcgtcctcca ccaggagcac ggtttcgtgg cccggcgggg
     4741 cccccgcctc gatcaccgac agggccgcgc tgtcctcggc ctcgaccgcc ggaatgtaga
     4801 tccggaagct cgtccccgcg ccgacctcgc tcaggaagtc gatgtaacct tcgctctgct
     4861 taatgatgcc gaacaccgtg gccagtccga gaccggtgcc cttgccgtat tccttcgtgg
     4921 tgaagaacgg ctcgaagacg cggctcttca cctccgggct catccccgtg ccggtgtccg
     4981 tgacgctcac cagggcgaac cggcccggcc ggctgccggg ccgcagccgg cagaactcct
     5041 catccagatc cgcgccgctg gtctcgatga tgaggcgccc gccttgcggc atcgcgtccc
     5101 gggcattcac ggccaggttg atgatcacct gctccatctg gcccgggtcc acgcggaccc
     5161 gcggcaggag cggggtgtag atggtggtca gcacgatgtc ctctccgatg agccgtcgga
     5221 gcatcttctc gatggtgctc accacctcgt tcaggtccac gatctccggc gtcaggatct
     5281 gcttgcggct gaaggccagg agctggcgcg tcagcgtggc ggcccgctgc ccggagtccc
     5341 ggatatccgc gacgtacgcc cgattggggt cttcggggcg cagccgaccc aggaggacgt
     5401 cgctgtagcc caggatgacg gtgagcatgt tgttgaagtc gtgggccacg cctccagcca
     5461 ggagaccgat ggcctccatc ttctgggact gattgagctg cgcctgaaga cgggcgtggt
     5521 cgtcctccgc cttcttgcgc tcggtgatgt cggagacgat gcccagggaa ccggcgagcc
     5581 ggttgtccgg ccccgggatg gggctggtgg atacgatggc ccacaggtcc gctccgtcct
     5641 tgcgccggta acgggtctcg tggccgggga gcgcctctcc ccgtcgggcg cggtccatcc
     5701 gctgacgcca agcctcccgg tcctccggca cgacgaagtg ctcgagcgga acaccgatca
     5761 tctcctgcgg cgcgtagccg agcatggcgg cgagccgctg gttcacgaac gtggcgcgtc
     5821 cctccgggtc cacggtccag atcccctcca gcgtggtctc cacgatgcgg cggtagcgct
     5881 cctcgctggc gcgcatggcc agctccgcct ggcgctgggc aatcgttata gcgatctggt
     5941 ccgcgagctg ctccaggaag gacaccagct cgatcgagaa ccggccggcc accggatcgt
     6001 tgagctgcaa gagaccgagc acctgtctgc cgtggcgcag aggaatgagc gccatggact
     6061 ggtatccggt ggccagacag cggctgcggt agcctccggg ccgggccgtg tccgcgaagc
     6121 gcgtgctggc cagggcgttg gtccagaagc ttcccttgtc cgtgaagcac gagagggccg
     6181 gatcgaagct gccgcgcagg acgtccccgc agaggcaggc gagctgcggc atgccctcct
     6241 catcccggga cggccggccg tcggccccgt gctcgcagag atggttctcc cgttcgacga
     6301 acgcggccgg gaacccctgg acttcgagat acgggaaatc ctccccctcg cgcagacgca
     6361 gccccacggc ggcgcagccc gtccaccgct ggacgatcgt gagcacgccg cggatgagct
     6421 cgtgcaggtg gccgcggtcg ttcaggagct ggaggagggc cagggtgacc tcccgctccc
     6481 gccggccgcg gcggatctcg gtgacgtcgt ggaaggtgcc gagaaggacc ttgcgtcccg
     6541 cgatctcgat cggcatggcc ttgacctcgg cctcccgtat ggcgccgctc ttggtgagga
     6601 tcagctcctc gatgacctgc cccgcctgct cgccccgatg ctgcacgaag gcgcgggaga
     6661 gccgggcgta gccctcctcc gaggggtgga tcttgacctg cggcgcgccg atcagctctt
     6721 cccgcgagta gcccgtgagg cgctggaacg cctggttgca gtccaggagg atccccgtgg
     6781 tggcgtccgc cacggccaga ccctccagag actcgtcgaa caggcgtcgg tagtgctcct
     6841 cctgctcccg cagggcgcgt tcgtcgttct tgcgctcggt gacatcgcgg tagttgacca
     6901 cgatggcgcg aaccgccgga tggtccagca tgttgctggt gacgcactcc gcccagcgcc
     6961 agcttccgtc ctcgtgccga aggcgaagga ggaaggtccg ggtgcctccg ggttccgcca
     7021 ccaggcctcg gtagtcgacc gccatggact ccacctcttc ggggtgcatg acgtcccgca
     7081 ggttgcggcc gacgatggcc tgaggggtgt agcccagcat ccgcgttacc gccggtgtcg
     7141 tgtacagcac gacgccatgg gcgtccacca gggttacggc gtccgccgcg ttttggacca
     7201 gggctcggaa gcgctcctcg ctgccccgaa gctgttcctg ggtctgctcg cgttcctgga
     7261 gcaggttcca ctcccggagg gctcgagtca cggtccgagg aagcgcgcca aaggcatcgg
     7321 gagacttgac cacgtaatcc aaggcgccgg ccttaagggc ctccacggcg gtcgtctcgt
     7381 tcccgaaact ggtcatgacg agcaccggaa agggcccaaa ccgcggggga ggcgtgagca
     7441 gctccgtggc ccggccgtcg ggcaggttga gatccaccag ggcgatgtcc ggaggctgct
     7501 ccgccgccag acgccggtat tcccggatgg tctccgccga gagtacctcc actcccaggc
     7561 cgccg
//
LOCUS       DASRZS010000388         3499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3671700, whole genome shotgun sequence.
ACCESSION   DASRZS010000388 DASRZS010000000
VERSION     DASRZS010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3671700"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VFH83_09465"
     CDS             complement(<1..820)
                     /locus_tag="VFH83_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6486635.1"
                     /translation="MKLTLRFLVILGLILAATVPVMSQAKINPDYLNASRADTLIIDQ
                     PYKLDTFDNWNPYVPGNAFGWGMAEIGMDDLMYLNYGDGKFVMWMADNVTANAANTVW
                     TVHLRKGITWSDGVPFTADDIIYTIQMQVKNDKLTNHFYWLEWLDNVAKIDDLTVRYT
                     LKKPNPRFNNERISGGVGVFPDIFIPKHIWEKVDDPTTFKNYDPAKGLPLGTGAYILA
                     KTTTNQTIMVLNDNWWGAKTGFQKLPLPKKVVYNYVGTEEVRTRTAIDNGFDSMQ"
     gene            complement(948..1688)
                     /locus_tag="VFH83_09470"
     CDS             complement(948..1688)
                     /locus_tag="VFH83_09470"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6486636.1"
                     /translation="MSKNPAPRFLDGLKAMGSRTSLLIATGALVLLAAAIVVIVVLAT
                     RPPQVVVMNAEDLIRQADAIGFSPATSVGQGLVELAPASARLPDPSPDLLPVGTKAPD
                     FQLKTVMGESVKLSSFRGKTVLLEFFATWCPHCQAEAPYVMKIFQALPPVGFEVIGIN
                     VDSEDPASVFAFDHYFGIPYRTLLDPGGRPGGYTRPGTPGPTTRAYKISILPAFYVID
                     PNGRVAWRADGERPTVQIVQELTKASGP"
     gene            1794..2222
                     /locus_tag="VFH83_09475"
     CDS             1794..2222
                     /locus_tag="VFH83_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6486637.1"
                     /translation="MMRLARGFGLALVLGMVLLPCRLFAEGAPDYKDPAQLLLLISNR
                     TEPYVLVDVRTAAEYEKGHMPTAVNIPYDTIGANPPTTEKSALIIVYCASGVRSARAS
                     KTLTGLGYGRVVDFGAFSRWQGPSLNGSDPGDCPCRVPQD"
     gene            complement(2229..>3499)
                     /gene="fucI"
                     /locus_tag="VFH83_09480"
     CDS             complement(2229..>3499)
                     /gene="fucI"
                     /locus_tag="VFH83_09480"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433514.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HET6486638.1"
                     /translation="AAASLRGRAYLAMGGMSMGIAGSIVDPRFLESWLGLRYEMVDMS
                     EFHRRIEEKIYDQQEYERALAWVRSNCQESSDRNPHPRSRAQKDADWEFVVKMTLIGR
                     DLMVGNPRLAELGFEEEALGHNALASGFQGQRQWTDQLPNGDFMETMLNTSFDWNGIR
                     QPYVCATENDFLNGIAMLFGHQLTGTAQIFSDVRTFWSPDAVKRVTGKKLTGMAEGGV
                     IHLINSGSTCLDGTGRQSRDGKPVMKQYWEITPAEAGACLDATHFCPASLEYFRGGGF
                     SSEFETKGGMPITLSRVNLVAGLGPVLQIAEGHTVELPGDVGRALMDRTDPTWPTTWF
                     APRVTGSGAFRDVYSVMNNWGANHGAACYGHVGGDLLALASMLRIPVAMHNVPAERVF
                     RPKYWANFGDAESVGADYRACAALGPLYGR"
BASE COUNT          598 a         1125 c         1114 g          662 t
ORIGIN      
        1 cctgcatgct gtcgaagccg ttgtcgatgg cggtgcgggt ccggacctct tcggtgccga
       61 cgtagttgta gaccaccttc ttggggagcg ggagcttctg gaagccggtc ttggcgcccc
      121 accagttgtc gttgaggacc atgatggtct ggttcgtggt ggtcttggcc aggatgtagg
      181 cgccggttcc caggggcagg cccttggccg ggtcgtagtt cttgaaggtg gtgggatcgt
      241 cgaccttctc ccagatgtgc ttggggatga agatgtcggg gaacactccg acgccgccgc
      301 tgatgcgctc gttgttgaac cgcggattgg gcttcttcag cgtatagcgg accgtcaggt
      361 cgtcgatctt ggcgacgttg tccagccact ccagccagta gaagtggttg gtcagcttgt
      421 cgttcttcac ctgcatctgg atggtgtaga tgatgtcgtc cgcggtgaag ggcaccccgt
      481 cgctccaggt gatgcccttg cgcagatgga ccgtccacac ggtgttggct gcgttggccg
      541 tgacgttgtc cgccatccac atgacgaact tgccgtcgcc gtagttcagg tacatcaggt
      601 cgtccatgcc gatctcggcc atcccccagc cgaacgcgtt gccggggacg tacggattcc
      661 agttgtcgaa ggtatccagc ttgtacggct gatcgatgat caacgtgtcc gcgcgtgagg
      721 cgttgagata gtcggggttg atcttcgcct gactcatcac gggtaccgtg gccgccagaa
      781 tcaggccaag aatcaccaga aaccttagcg taagcttcat gccaacctgt tctcctttca
      841 aggtggggtt tccgtactat ataatggcgc ccgaccggtg tcaaacttat tccaatcttg
      901 aaatataaca gaccgatact agcccgccga acggccaacg cccggcgtca gggccctgat
      961 gctttggtaa gctcctggac gatctgaacg gtcggtcgtt ctccgtcggc gcgccaggca
     1021 acccgtccgt tgggatcgat tacgtagaac gccggcagaa tggagatctt gtaggctcgg
     1081 gtggtcggcc ccggggttcc gggccgtgtg tagccgccgg gccgaccacc cggatccagc
     1141 agggtccgat acgggatccc gaagtagtgg tcgaacgcga agacgctggc gggatcctca
     1201 ctgtccacgt tgatgccgat cacctcgaat cccaccgggg ggagcgcctg gaagatcttc
     1261 atcacgtatg gtgcctctgc ctgacagtgc gggcaccagg tggcgaagaa ctcgaggagc
     1321 acggtctttc cccgaaagct cgaaagcttc accgactccc ccatcacggt cttgagctgg
     1381 aagtccggcg ccttcgttcc aaccgggagc aggtccggac tgggatccgg aagcctggca
     1441 ctggccggag ccagctccac caaaccctgt cccacggagg tggccggcga gaatccgatg
     1501 gcgtccgcct ggcggatcag gtcctcggcg ttcattacga ccacctgggg aggacgcgtg
     1561 gccagcacca caatgaccac gatggccgcc gccaggagca cgagcgcacc ggtggcgatc
     1621 aggagactcg ttcgagatcc catggccttg agcccgtcca ggaagcgtgg cgccggattc
     1681 ttcgacatgg cgggtagatt agccgcgggt ccggttcagg tcaacgcgcc gattgacacg
     1741 acgacagcgg cggccgaaaa tgcgcaatct ggcgcgcgga aaaggattcc accatgatgc
     1801 ggcttgctcg cggcttcggc cttgccctcg tccttgggat ggtgctcctg ccctgccggc
     1861 ttttcgccga gggcgcccct gactacaagg acccggccca gctcctgctc ctgatctcga
     1921 accgtaccga gccttacgtc ctggtcgatg tccgcacggc cgcggaatac gagaagggcc
     1981 acatgccgac cgcggtcaac attccctatg acaccatcgg cgccaatccc ccgaccaccg
     2041 agaaatcagc gctcatcatc gtttactgtg cgtcaggggt tcggtccgcc agggcgtcca
     2101 agaccctgac ggggctcggc tacggacgcg ttgtggattt cggagcgttc tctcggtggc
     2161 aggggccgtc cctcaacgga agcgatccgg gggactgccc ctgcagggtt ccgcaggact
     2221 agggaacttt accggccata aagggggccc agcgcggcgc aggcccgata gtccgcgccc
     2281 acggactccg cgtccccgaa gttggcccag tacttgggtc tgaacacccg ttccgccgga
     2341 acgttgtgca tcgcgacggg aatcctgagc atcgaggcga gcgccagcag gtctcccccc
     2401 acgtgcccgt agcaggccgc gccgtggttg gcgccccagt tgttcatcac cgagtagaca
     2461 tcccggaagg ctccactacc ggtgacgcgc ggggcaaacc aggtggtagg ccaggtggga
     2521 tcggtccgat ccatcagggc gcggcccacg tcccccggca gctccaccgt atgaccttcc
     2581 gcgatctgga gaaccggacc cagaccggcg accaggttca cgcgggagag cgtgatgggc
     2641 atcccgccct tggtctcgaa ctccgaggag aagccgccgc cgcggaagta ctccagcgag
     2701 gcgggacaga agtgcgtggc gtccaggcac gcccccgcct ccgccggggt gatctcccag
     2761 tactgcttca tgaccggctt gccgtcccgg ctctgtcggc cggtcccatc cagacacgtg
     2821 gagccggagt ttatgaggtg gatgacgccg ccctccgcca tgccggtgag ctttttcccg
     2881 gtgacgcgct tgaccgcgtc cgggctccag aacgtccgca cgtcggagaa gatctgcgcc
     2941 gtgccggtga gctggtgtcc aaagagcatg gcgatgccgt tcaggaagtc gttctccgtg
     3001 gcgcagacgt acggctggcg tatgccgttc cagtcgaagc tggtgttcag catggtctcc
     3061 atgaagtcgc cgttgggaag ctggtccgtc cactgccgct ggccctggaa cccggaggcc
     3121 agtgcgttgt gacccagcgc ctcttcctcg aaccccagct ccgccagccg ggggttgccc
     3181 accatcaggt cccgaccgat cagggtcatc ttgaccacga actcccagtc ggcgtccttc
     3241 tgggcccggc tccgcggatg gggattgcgg tccgaggact cctggcagtt cgagcggacc
     3301 cacgccagcg cccgctcgta ctcctgctgg tcgtagatct tctcctcgat gcggcggtgg
     3361 aactcgctca tgtccaccat ctcgtagcgc agccccagcc agctctcgag gaaccgggga
     3421 tccacgatgg agccggcgat ccccatcgac atgccgccca tggccaggta cgcgcgaccc
     3481 ctcagggacg ccgcggcca
//
LOCUS       DASRZS010000389         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1748396, whole genome shotgun sequence.
ACCESSION   DASRZS010000389 DASRZS010000000
VERSION     DASRZS010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748396"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..>2019
                     /locus_tag="VFH83_09485"
     CDS             13..>2019
                     /locus_tag="VFH83_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET6486639.1"
                     /translation="MSRRDALNADPRDARMVASTPIITRTGGYGAVYVSGADSARATV
                     KRYAREGFDLIKIAIEDDLQGRRWPMLDAAEVNALVEAAHAAGKRVSAHVTHARNLAL
                     AVDAGVDDIAHMVVEPISEETARRIASKGILWVPTLELWKGVSEAYSLDWIEVAKRNT
                     ATFVRAGGRIALGTDYNGYTTHFDSGFPLTEARLLLESGLSPSEVIVAGTRNAAEACG
                     LSADLGTVQRGKVADLLVVRRNPLEDITALAEPEQVYKSGLPVLTGASEPAAVPADDL
                     ASLDASIPQIMKQQQVPGLAVAITDDKGTLWSKAYGRTDWRRGRAVDQDTIFGALPGS
                     GAITATAVLMACRDGLVDLDAPISSYLPGFRINSVFQEHPERAITLRQLLRGTAGLPQ
                     EAPYGNNYDLNAVDVSKHRESISEAWLRFPAGMNWAYSNLGLDLAAYVLQERSGMPYP
                     QYVDKVVFAPLGMKRATFDPDSILADTNRAIGHQPFQSSVPPIVPMMGAGGAYASASD
                     LAKLVRFHLGRGAFDGDRLVDEKWLLPMYALDPTAGSAGLGVFKSFLTLQGHNRTFGF
                     GTSGGGFGFLNAMLWYPRLGIGGVVLSSSGGGSMAWEVLNQALDAVIDDPRTVYHDRL
                     AAIAENDPQGGLPQGDPVGSLASENRLRLSRIVGRHSPEWATAWS"
BASE COUNT          335 a          658 c          704 g          322 t
ORIGIN      
        1 tctcccgacc tggtgtcccg gagggacgcc ctcaacgcag accctcggga tgcgcggatg
       61 gtggccagca cgcccatcat cacgcggacc ggtggctacg gcgcggtgta cgtgagcggc
      121 gcggattccg ctcgggcgac ggtgaagcgg tatgcccggg aagggttcga cctcatcaag
      181 atcgccatcg aggatgacct gcaaggcagg cggtggccga tgctggacgc cgcggaagtg
      241 aacgccctgg tcgaagcggc gcacgccgcg ggcaaacggg tgtcggctca cgtcacccac
      301 gcgcggaacc tcgctctcgc cgtggatgcg ggcgtggacg acatcgcgca catggtggtg
      361 gagcccatct ccgaggaaac ggcacgtcgg atcgcttcca agggcatcct gtgggtgccg
      421 accctcgagc tctggaaggg cgtgagcgaa gcgtactccc tcgactggat cgaggtcgcc
      481 aagcggaaca cggccacctt cgtccgggcg ggaggcagga tcgcgctcgg caccgactac
      541 aacggctaca ccacgcattt cgattcgggg ttcccgctga cggaagccag gctgctgctg
      601 gagtccggcc tttctccctc ggaggtgatc gtggcgggga cccggaatgc cgcggaggcc
      661 tgcggactgt cggcggacct gggaaccgtg cagagaggga aggtggctga tctcctggtc
      721 gtccggagga atcccctgga ggacatcacg gcgctcgcgg agcccgagca ggtatacaag
      781 tccgggcttc ccgtgctcac cggagcctcg gaaccggcgg cggttccagc ggacgacctg
      841 gcatccctgg acgcctccat cccgcagatc atgaagcagc agcaggtccc cggattggca
      901 gtggcaatca cggacgacaa gggcacgctc tggagcaagg cctacggtcg gacggactgg
      961 cgtcgaggtc gggcggtgga ccaggacacg atcttcggcg cgctgcccgg ctcgggagcc
     1021 atcacggcca ccgcggtcct gatggcctgc cgcgacgggc tcgtggatct ggacgcgccg
     1081 atctcgtcct atcttccggg gttccggatc aacagcgttt tccaggagca ccccgagcgg
     1141 gccatcaccc ttcgccagct ccttcgcggc accgcgggtc tgccccagga ggccccctac
     1201 ggcaacaact acgacctgaa cgccgtcgac gtctcgaaac atcgggagag catctccgaa
     1261 gcctggctgc gctttccggc gggcatgaac tgggcctatt cgaacctggg gctggacctc
     1321 gcggcttacg tgctgcagga gcgctccggg atgccgtacc cgcagtacgt ggacaaggtg
     1381 gtctttgcgc ccctgggcat gaagcgggcg acctttgacc cggattcgat cctcgcggat
     1441 accaaccggg ccatcggcca ccagccgttc cagagcagcg tgccgcccat cgtcccgatg
     1501 atgggcgccg gcggggctta cgccagcgcc tcggatctcg cgaagctcgt gcggttccat
     1561 ctgggcagag gggccttcga cggcgacaga ctggtggacg agaagtggct gctgccgatg
     1621 tacgctctcg acccgaccgc gggaagcgcg ggtctgggcg tcttcaagag cttcctcacc
     1681 cttcagggcc acaaccgaac gttcggattc ggcaccagcg gcgggggctt cgggtttctc
     1741 aacgccatgc tgtggtaccc cagactgggg atcggcgggg tggtgctttc gagctccggc
     1801 ggcggctcga tggcctggga ggtcctgaac caggccctgg acgcggtgat cgacgatcct
     1861 cgaaccgtct accacgatcg actggcggcg attgcggaaa acgatccgca gggcggcctt
     1921 ccgcaaggcg atcccgtcgg atcccttgcg agcgagaacc ggctgcggct ctcgcggatt
     1981 gtcggacggc actccccgga gtgggcgacg gcgtggagc
//
LOCUS       DASRZS010000390         4844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_600880, whole genome shotgun sequence.
ACCESSION   DASRZS010000390 DASRZS010000000
VERSION     DASRZS010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600880"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFH83_09490"
     CDS             complement(<1..500)
                     /locus_tag="VFH83_09490"
                     /inference="COORDINATES: protein motif:HMM:NF020031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET6486640.1"
                     /translation="MKIHEYQAKAFLKEYGVPVPEGRAVATPEEAGAAAQSIGCPVMV
                     KAQVLVGGRGKAGGVKMARTVEEAEARAREILGMKIKGIVVQKVLVASAVDIAAEFYL
                     SLTVDRAARAIQCIASASGGMEIEEIAKTQPDRIVTFSVPAARGLDRKAHFQELSRAF
                     APAAVA"
     gene            complement(497..1420)
                     /gene="mdh"
                     /locus_tag="VFH83_09495"
     CDS             complement(497..1420)
                     /gene="mdh"
                     /locus_tag="VFH83_09495"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET6486641.1"
                     /translation="MKVSVIGAGNVGAKAVYCIAEKNVAETVMVDVVDGLARSKALDF
                     LHAAPFRGYEGTIRGTTDFAAIADSDVVVLTAGVARKPGMDRMDLLKTNVAIARQSAR
                     EIAQRAPNAVVIVVTNPLDVIAMVVLRETGFALKRVVGMAGVLDSTRFRYFIAERTGM
                     WPGDVQAMVLGGHGDEMVPLPAYTCVAGVPINQILDPASIEELVKRTRTGGAEIVSLL
                     RTGSAYYAPGASVAKMVEAVLKDEKRLLPASTYLRGEYGFRDIFLGVPVILGRNGVEK
                     IVELPLTESERRALEGSAEAVRKGLKEAEGL"
     gene            complement(1417..2364)
                     /locus_tag="VFH83_09500"
     CDS             complement(1417..2364)
                     /locus_tag="VFH83_09500"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET6486642.1"
                     /translation="MAGVSIIGSGNVGANAAFFIAEKGVTDVLMVDIATGLSRGKALD
                     VMEAAPIRRYRNTISGSDSLEDIRGSPVIVVAAGAIRAPGMRREELYGANAGLIRDLA
                     PRIARLAPDATVLMATEPVDGLTTLFVRASGMPRTRVLGLGGVLDSTRLRALIALELQ
                     VSSEIVSAMVIGRHSDDMVCLPRYCSVSGVPLPLLLPQPRIASLVEQTRSAGDLVVEL
                     AKRSTAYYAPSAAIAEIADSIHMDLKRVFSLSILLQGEYGCQDAALSLPVVIGEKGAA
                     RVLTPELLVDEVAVLRKSAKLIADVVGQEPRRASEGGVG"
     gene            2558..3286
                     /gene="atpB"
                     /locus_tag="VFH83_09505"
     CDS             2558..3286
                     /gene="atpB"
                     /locus_tag="VFH83_09505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET6486643.1"
                     /translation="MASLGAETLVQLRGIPITNSLVASVVVDAVILAVVFGVRRRLSL
                     VPGRFQGVVESVMGFLSTTVDQVAGPRAKQIFPWVAIFFIYIVFSNLIGLLPGFGSIG
                     FQRGDEFVPLLRGSTADLNETLALAVVSVIATHAYAIRTIGLKSYLTRFFALSAFFFV
                     GLLELVQELTKFISFSFRLFGNISGGEMVLGAMSSLFAFIVPVPFLALEILVAVIQAM
                     IFSLLTMAFMTILSEPHEGGGHSA"
     gene            3283..3495
                     /locus_tag="VFH83_09510"
     CDS             3283..3495
                     /locus_tag="VFH83_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HET6486644.1"
                     /translation="MNYAITLTGGVIAAIGGLGPGIAIGLIGMKAMEAIGRNPEASGR
                     IVPIMILAMAFAEAIAIYSLIFAFLR"
     gene            3520..4029
                     /gene="atpF"
                     /locus_tag="VFH83_09515"
     CDS             3520..4029
                     /gene="atpF"
                     /locus_tag="VFH83_09515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET6486645.1"
                     /translation="MEQLGSFHLNVVLLVAQIVNFLIIGWVIYRFLIKPMLATLDKRR
                     ATIAKGLEDAEKARVALEQAGVDREKVLERAHEEASEVLRAARAEADRIRSDAQDRAR
                     GDADRVLAEARSVIALERQEMERAVRRLSLDLSGRILSTVVGDLFSGAEKDSIVARGL
                     ERIGKAGVV"
     gene            4026..4364
                     /locus_tag="VFH83_09520"
     CDS             4026..4364
                     /locus_tag="VFH83_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486646.1"
                     /translation="MKRLVELSFTAGGELDDRVVGMILDRLSRSQLKQYRTALLREVR
                     RRSVKVTVEGGTDRTVGDAMKGRFPERQVEIAHDPALGAGVRLTSGDDVEDASVSGYV
                     RSIIEQLGAP"
     gene            4361..>4844
                     /locus_tag="VFH83_09525"
     CDS             4361..>4844
                     /locus_tag="VFH83_09525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014873.4"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET6486647.1"
                     /translation="MKAQDLVREIEERLSTVAVRPGVTNVGHVISVGDGIIRVDGLSR
                     AGFGEQVELEDGRRGLILHLEEDYASVLMLSEDTGVPEGMEVRTTGKLLSLRIGEELL
                     GRVVDPFGNPLDDRPLEVTSGEDRPLERIAPGVTERKPVDQALKTGIKAVDALTPIGR
                     G"
BASE COUNT          772 a         1640 c         1599 g          833 t
ORIGIN      
        1 tccgcaacgg ccgcgggggc gaacgcgcgc gagagctcct ggaagtgggc cttccgatcc
       61 agaccgcgcg ccgccggcac ggagaaggtc acaatgcggt ccggctgggt cttggcgatc
      121 tcctcgatct ccatgcctcc cgacgcgctg gcaatacact ggatggcccg cgccgcccgg
      181 tccacggtga ggctgaggta gaactccgcg gcgatgtcca ccgcggatgc gacgagcacc
      241 ttctgcacga cgatcccttt gatcttcatg ccgaggatct cccgcgcccg ggcttccgct
      301 tcctccacgg tcctggccat cttcacgccg ccggcttttc cgcggccgcc caccagcacc
      361 tgtgccttca ccatcacggg acagccgatg ctctgcgcgg cggcgcccgc ctcttcgggc
      421 gtggccactg cgcggccctc cggtacggga acgccgtatt ccttgaggaa cgcctttgcc
      481 tggtactcgt ggatcttcac agtccctccg cctccttcaa ccccttgcgc accgcctccg
      541 ccgatccttc cagcgcgcgt cgttcggatt cggtgagcgg aagctcgacg atcttctcca
      601 cgccgttgcg tcccaggatg acggggaccc ccaggaagat gtctctgaat ccgtactcac
      661 cccgcagata ggtcgacgcg gggaggaggc gcttctcatc cttgagcacc gcctccacca
      721 tcttggccac cgacgcgccc ggcgcgtagt aggcgctgcc ggtccgcagg aggctgacga
      781 tctccgcgcc ccccgtcctg gtccgtttca ccagctcctc gatggaggcc gggtccagga
      841 tctggttgat gggcacgccc gccacgcacg tgtaggccgg cagggggacc atctcgtctc
      901 cgtgcccgcc cagcaccatg gcctggacat cccccggcca catgcccgtc cgctccgcga
      961 tgaagtagcg aaaccgcgtg gagtccagga cgccggccat gcccaccacc cgtttgagcg
     1021 cgaaccccgt ctcccgcagc accaccatcg ctatcacgtc caaaggattg gtgaccacga
     1081 tgaccaccgc gttgggagcg cgctgggcaa tctcccgggc cgactggcgc gcgatggcca
     1141 cgttggtctt gaggagatcc atccggtcca tgcccggttt gcgcgccacg cccgcggtca
     1201 gcaccaccac gtcgctgtcc gcgatggcgg cgaagtccgt ggtccctcgg atggtgcctt
     1261 cgtagccgcg gaacggcgcc gcgtgcagga agtccagcgc cttggagcgc gccagcccgt
     1321 ccacaacgtc caccatgacg gtctccgcca cgttcttctc cgcgatgcaa tagaccgcct
     1381 tggccccgac gttgcccgcc cctatgacgg agaccttcat ccgacgcctc cttccgacgc
     1441 gcgccggggc tcctgcccga ccacgtccgc gatgagcttg gcggatttcc gcagcacggc
     1501 cacctcatcc acgaggagct ccggggtcag gacccgcgcg gcgcccttct ccccgatcac
     1561 cacggggaga ctgagcgccg catcctggca gccatattcc ccctgcagga ggatcgacaa
     1621 cgagaacacc cgcttgaggt ccatatggat ggaatccgca atctcggcga tggcggcgga
     1681 aggggcgtaa taggccgtgg agcgcttggc aagctccacc accaggtctc ccgcgcttcg
     1741 ggtctgttcc accagcgagg cgatgcgggg ctgcgggaga aggagcggca agggcacacc
     1801 ggacacgctg cagtagcgcg gcaggcacac catgtcatcc gagtggcggc cgatcaccat
     1861 ggcggacacg atctccgaag agacctggag ctccagggcg atcagggccc gcaggcgcgt
     1921 gctgtccagc acgccaccca ggccgagcac ccgggtgcgc ggcatcccgg aggctcggac
     1981 gaacagcgtc gtcagcccgt ctaccggctc cgttgccatg aggaccgtgg catccggagc
     2041 cagccgcgca atgcggggcg ccagatcccg gatgagaccg gcgttggcgc cgtacagctc
     2101 ttcccgccgc atgcccggcg cgcggatggc gccggccgcc accacgatga cgggagagcc
     2161 gcggatgtcc tccagggagt cgctgccgga gatggtgttc cggtatcggc ggataggcgc
     2221 cgcctccatg acgtccagcg ccttgcctcg ggaaagcccc gtggcgatgt ctaccatgag
     2281 cacgtccgtg acgcccttct cggcgatgaa gaacgcggcg ttggctccca cgtttccgct
     2341 gccaatgatg ctcactccgg ccatggctca cctctctcgc ctggtgcctc gtgtcgtccg
     2401 ggcgcagcct tcgccccgac gctctacgcg cgagaccgcg atgaacggcg tacccctagt
     2461 atacttattc gcgagcggag gagcgagtac aaacagctat tcgacgcaca atcgttcgtt
     2521 gactcgggag ggcgtcgatt gtatcttccc caatgccatg gcgtcgctcg gagctgaaac
     2581 cctcgtccaa ctccgcggga ttccgatcac gaacagcctt gtggcctccg tcgtggtcga
     2641 tgccgtcatc ctggcggtgg tctttggcgt ccgacgccgg ctctcgctgg tgcccggacg
     2701 gttccaaggc gttgtcgaat cggtcatggg cttcctctcc accacggtgg accaggtggc
     2761 gggaccccgg gccaaacaga tctttccctg ggtggcgatc ttcttcatct acatcgtgtt
     2821 ctccaacctc atcggcctgc tgccgggatt cggttccatc gggttccagc ggggagacga
     2881 gttcgtgccc ctgttgcggg gcagcaccgc ggacctcaac gagaccctgg ccctggcggt
     2941 ggtgtccgtg atcgcaaccc acgcgtacgc catccggacc atcgggctga agtcctacct
     3001 gacgcgcttc ttcgcgttga gcgcgttttt cttcgtcggt ctcctggagc tggtccagga
     3061 gctgaccaag ttcatttcgt tctcgttccg gttgttcggc aacatctcgg ggggcgagat
     3121 ggtgctcggc gccatgtcct ccctgttcgc cttcatcgtt cccgtgccat tcctcgcgct
     3181 ggagatcctg gtagcggtca tccaggcgat gattttttcc ttgctcacga tggcattcat
     3241 gacaatcttg tcagaaccac atgaaggggg agggcacagc gcatgaacta cgcaataacc
     3301 ttgaccggcg gcgtgatcgc cgcgatcggc gggctggggc ctggcatcgc catcggcctg
     3361 atcgggatga aggccatgga ggccatcggc cggaaccccg aggcctcggg gcgcatcgtg
     3421 ccgatcatga tcctcgccat ggcgttcgcc gaggccatcg ccatctactc gttgatcttc
     3481 gcgttcctca gatagcttcc cgcagggagg gcgtctccta tggagcaact gggcagcttc
     3541 cacctgaacg tcgtgctgct ggtggcccag atcgtcaact tcctgatcat cgggtgggtc
     3601 atctaccgct tcctgatcaa gcccatgctg gccacgctgg acaagcggcg cgccaccatc
     3661 gccaagggac tggaggacgc ggagaaggcc cgggtggctc tggagcaggc gggcgtggac
     3721 cgcgagaagg tgctggagcg cgcgcatgag gaggcctccg aggtccttcg ggcggcgcgc
     3781 gccgaagccg accggatccg ctcggacgcc caggaccggg cccgcgggga cgcggaccgc
     3841 gttcttgcgg aggcccgttc cgtgatcgcc ctggagcggc aggagatgga acgggcggtg
     3901 cgccggctca gcctggacct ctcgggacgc atcctgtcca cggtcgtggg agacctcttc
     3961 agcggcgcgg agaaggatag catcgtggcc cgcgggctgg agcggatcgg gaaggccggc
     4021 gtcgtgtgaa gcggctggtg gagctgtcct tcacggcggg cggcgagctg gacgaccgcg
     4081 tggtcggcat gatcctggat cgactgagcc ggtcgcagct caaacagtac cggaccgcgc
     4141 tgcttcggga ggtccggcgg cggagcgtga aagtcaccgt ggaaggcggc acggaccgca
     4201 cggtggggga cgccatgaag gggcggttcc ccgagcggca ggtggagatc gctcatgacc
     4261 ccgccctggg agcgggagtg cggctgacgt ccggagacga cgtggaagac gccagcgtca
     4321 gcggctacgt ccgttctata atagaacagc ttggagcgcc atgaaggcac aggacctggt
     4381 tcgggagatc gaagagaggc tgtcaaccgt cgccgtgcgg cccggcgtga caaacgtggg
     4441 gcacgtcatc agcgtgggag acgggatcat ccgcgtggat gggctctccc gcgcggggtt
     4501 cggggagcag gtcgagctgg aggacggccg gcgcggcctg atcctccatc tggaggagga
     4561 ctacgcctcc gtcctgatgc tctcagagga cacgggggtc ccggagggca tggaggtccg
     4621 caccacgggc aagctcctgt ccttgcggat cggggaagag ctgttgggcc gcgtcgtaga
     4681 tccgttcgga aaccccctgg acgatcgtcc gctggaggtg acctcgggtg aggaccgtcc
     4741 gctcgagcgg atcgcaccgg gggtcacgga gcgcaagccc gtggaccagg ccctgaagac
     4801 cgggatcaag gcggtggacg ccctcacccc gatcggcagg ggcc
//
LOCUS       DASRZS010000391         4447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5601987, whole genome shotgun sequence.
ACCESSION   DASRZS010000391 DASRZS010000000
VERSION     DASRZS010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5601987"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFH83_09530"
     CDS             <1..280
                     /locus_tag="VFH83_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486648.1"
                     /translation="ERPEAIRTVRRVVGSSDLLIIKCQRVLQRERMAGNLDDRRFSES
                     ADVLGAGVDRMLLIEMGVAAKRRAAGPLPTVIGTLDASTGPAPPCGSP"
     gene            complement(295..762)
                     /locus_tag="VFH83_09535"
     CDS             complement(295..762)
                     /locus_tag="VFH83_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6486649.1"
                     /translation="MKPVVFRLLLAFAAAALMPSLVALLSGCQGSPGSRVARPASAQP
                     VDYSDPDRLYQLVSKQMEKYVLVDVRTKEEYLQSHIPTAVNIPYYVIGQSLPATDRSS
                     LIIVYCSSGVRSAAAKTALDKLGYTNVVDFGAMSRWKGAVLTSYDPGKCPCNL"
     gene            complement(769..1539)
                     /locus_tag="VFH83_09540"
     CDS             complement(769..1539)
                     /locus_tag="VFH83_09540"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6486650.1"
                     /translation="MGKLDSNVAIVTGSTSGIGAAAARRLAAEGARVVVTGRDEARGS
                     RVTAEIRSSGGTALFVRADVSREEDCRRIVETTVRELGRLDILVNNAGTTSTVVLEEA
                     DEAEFDRILGVNLKSIFFACKYALPVMKRQRHGVIVTVASRGAIMPTHCSAIYAASKA
                     AALQMTQAIGVNYGAYGIRANTILPSYIDTPMTDEFIQKSGQDYKAAIRALEEHTPVG
                     RIGTSEDCANAILFLASEEASFITATPMLVDGGAVFGG"
     gene            complement(1544..2857)
                     /locus_tag="VFH83_09545"
     CDS             complement(1544..2857)
                     /locus_tag="VFH83_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6486651.1"
                     /translation="MNARERMLASLAHRQTDRVPTFELAFGTRLATRVLGRPAFFPRS
                     GGSALRDILLANARGTQARRDVIRAGTLTQIELYTALGYDATCIIPTEFLQTVFESFG
                     LFGTNYFLDVTIEELKPSTWRVSAPGGFWSIHAYDERADVFYAVRDSVSEGGIPALRR
                     YVDELSSRSTAINAHVADALESVRIAVQTPEVRGGQLFFLGHCDICMPTADAFLPVFL
                     EAMALEPVLVERYFQVTTDGLMPILSAQIDLGVDGIVGANDWCFKTGPMMSPSMFRRF
                     LVPSLKRIVDAVHARGLPFVKHLDGNTREILPILVDEVGIDAYHSVEPSAGMDIRELK
                     RLYGGRISLWGNLDCGDLLVHHPPEDVARAADALIRDLSPGGGYVLSSSNSIHDGVPP
                     ENLMAMLSAAQGRPAVAGLAALAAKAGEVGQDRAAVAGHIDEGRQ"
     gene            complement(2854..3834)
                     /locus_tag="VFH83_09550"
     CDS             complement(2854..3834)
                     /locus_tag="VFH83_09550"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET6486652.1"
                     /translation="MSMMSMAQAISEAQAEEMERDERVFVLGEDVGVMGGAFNATDGL
                     FKRFGCKRVIDMPISEAGILGAGIGAALAGMRPVVEMQYGEFLGYMDPLANHMSKMHY
                     MTRGRVRLPMVLRLPNGGKLGNAAPHSQYLEAWFMHMPGLRVAMPSNPRDAKGMLKAA
                     IRSDDPVVFIEDKLLYPRTGEVPEGEVVPLGKAAVANPGTAITVVAVGWTVSRALAAA
                     RELEAEGIQIEVIDPRTLNPLDMDTIVESVRKTGKAIVAHQACRTGGVGAEISARIAE
                     DAFEYLDAPVKRVTGMDCPIPYNIELEKMSLPDEKEIAQAVRDLVGREHG"
     gene            complement(3831..>4447)
                     /locus_tag="VFH83_09555"
     CDS             complement(3831..>4447)
                     /locus_tag="VFH83_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET6486653.1"
                     /translation="GIGMAITMQKGSEVVGCAFGDGATNAGGCHEGMSIAAIHKAPVV
                     FVCENNGMAVSTPIQGVLPVRRISDRAVAYGMPGRTVDGTDAVEVLQAMDEFVSRARN
                     GDGPSFLEATCYRWHGHTVWDTSGYRTKEENEAWMTHDPIPKLAEYLKSHGALADTED
                     GSWKQEIRQVIEEAVQFAKTSPAPEGTREDIQRFVYVESQEAVG"
BASE COUNT          734 a         1529 c         1406 g          778 t
ORIGIN      
        1 ggagcgtcct gaggcgattc gcacggttcg acgggttgtt ggctccagtg acctgctgat
       61 catcaaatgc cagcgcgtgc ttcagcgaga gcggatggcc ggcaatcttg acgaccggcg
      121 attctccgaa tcggcagacg tgttgggcgc cggggtggac cggatgctac tcatcgagat
      181 gggtgtagcg gccaagcgtc gggccgccgg tccgttgccg accgtcatcg ggaccctcga
      241 tgcgtccacc gggccagcgc cgccctgtgg cagcccatag ggtcgagccg cagcctacag
      301 attgcagggg cacttcccgg gatcgtagct tgtgaggacc gcacccttcc agcgagacat
      361 cgcgccgaaa tccaccacat ttgtgtagcc cagcttgtcc aacgccgtct tggccgcggc
      421 ggatctcacc ccagaggagc agtacacgat gatgagcgag gacctgtcgg tggcggggag
      481 actctgcccg atcacgtagt acggaatgtt caccgcggtc ggtatgtggg attggaggta
      541 ctcctccttc gtccgaacgt cgaccaggac atacttctcc atctgcttcg aaacgagctg
      601 atagagccgg tctgggtcag agtagtcgac gggctgcgca gaggcaggcc gcgccacgcg
      661 actgcctggc gagccctgac acccgctcag gagcgccacg aggctgggca taagcgccgc
      721 cgcagcgaag gccagcagca aacgaaacac aacgggcttc accggccatc atcctccgaa
      781 gacggcaccc ccgtccacga gcatcggggt cgccgtgatg aagctggcct cctccgatgc
      841 caggaagagg atggcgttgg cgcaatcctc cgaagtcccg atccggccca cgggtgtgtg
      901 ctcctccaag gctcggatag cggccttgta gtcctgcccg gatttctgga tgaactcgtc
      961 ggtcatcgga gtgtcgatgt agctgggcag gatggtgttt gctcgtatgc cgtacgcccc
     1021 gtagttgacc ccgatggcct gggtcatctg aagggcggcg gccttggagg ccgcgtagat
     1081 cgcgctgcag tgcgtcggca tgatggcgcc ccgggaggcg accgtgacga tgacgccgtg
     1141 gcgctggcgc ttcatgacag gcagcgcgta cttgcacgca aagaagatgc tcttcaggtt
     1201 gactccgagg atccgatcga actcggcttc atcggcctct tccaggacga cggtgctggt
     1261 ggtccccgcg ttgttgacca ggatgtccag tctcccgagc tcgcgaaccg tggtctccac
     1321 tatgcggcgg caatcctcct cccgggaaac gtctgcgcgc acaaagaggg ccgtcccacc
     1381 ggagcttcgg atctccgccg tgaccctgga ccctcgagcc tcatcgcgtc ccgtgaccac
     1441 cacgcgggcc ccctcggctg ccagccttcg ggcagcggcg gccccgatcc cgctcgtcga
     1501 gccggttacg atcgccacgt tcgaatccag cttccccatg cgcctactgc ctcccctcat
     1561 caatgtgccc ggcgaccgcg gcccggtcct ggccgacttc ccccgccttc gccgcgagcg
     1621 cagcaagccc cgcgaccgcc ggtcgcccct gggccgcgga gagcatcgcc atcaggttct
     1681 ccggcgggac cccatcgtgg atggagttgc tggacgacag gacgtagccc cccccagggg
     1741 acaggtctcg gatcagcgcg tcagccgccc gggccacatc ctccggcgga tgatgcacca
     1801 gcaggtcgcc gcagtcgagg ttgccccaca gcgagatccg tccgccgtag agcctcttca
     1861 gctcgcggat gtccatcccc gccgagggct ccaccgaatg gtaggcgtcg atccccactt
     1921 catcgacgag gatgggaagg atctcccggg tgttgccgtc caggtgcttc acgaacggga
     1981 gtccgcgggc gtgcaccgcg tccacgatcc gcttcagcga agggaccagg aacctgcgga
     2041 acatcgacgg cgacatcatg ggcccggtct tgaagcacca atcgttggcc cccacgatgc
     2101 catcgacacc caggtcgatc tgcgcgctca ggatcggcat caacccgtcc gtggtcacct
     2161 ggaagtaccg ctccacgagc acgggctcca acgccatggc ctccaggaac accggtagaa
     2221 acgcgtccgc ggtgggcata cagatgtcgc agtgccccag gaaaaagagc tggccgcctc
     2281 gaacctccgg ggtctggacc gcgatacgga cagactccag agcgtcggcg acgtgtgcgt
     2341 tgatcgccgt gcttcgggag gagagctcat cgacatagcg gcgaagagcc gggatcccgc
     2401 cttcgctgac cgagtccctt accgcgtaga agacgtccgc gcgctcgtcg taggcgtgaa
     2461 tggaccaaaa ccctcccggg gcgctgacac gccaggtact gggcttgagc tcctcgatgg
     2521 tgacatccag gaagtagttg gtcccgaaga ggccgaagct ctcgaagacg gtctggagga
     2581 actcggtcgg gatgatgcag gtggcgtcgt agcccagcgc cgtgtacagc tcgatctggg
     2641 tcagggtgcc ggctcgaatg acatcgcgtc gcgcctgcgt gcccctggcg ttggccagga
     2701 gaatgtcgcg cagcgccgag cccccgctcc gcgggaagaa ggcaggccgg cccaggacac
     2761 gcgtggccaa tcgggtccca aacgccagct cgaaggtcgg gacccgatcg gtctgccgat
     2821 gagccaggct ggccagcatc cgctcccgag cgttcatcca tgctcccggc ccacgagatc
     2881 gcgcaccgcc tgcgcgatct ctttctcatc gggcagggac attttctcca gctcgatgtt
     2941 gtaggggatg gggcagtcca tgccggttac cctcttcacc ggagcgtcga gatactcgaa
     3001 cgcgtcctcc gcgatccgcg cggagatctc tgcccccacg ccgccggtcc gacaggcctg
     3061 gtgagccacg atggctttgc cggttttcct gacgctctcg acgatcgtgt ccatgtccag
     3121 cggattgagg gtccgcgggt cgatcacctc gatctggatt ccttcggcct ccagctctcg
     3181 cgccgcggcc agggcccggg agacggtcca cccgacggcg accaccgtga tggccgtgcc
     3241 cggattggcc acggctgcct tccccagggg caccacttct ccttcgggga cctcgccggt
     3301 ccgagggtag agcagcttgt cctcgatgaa gaccaccggg tcgtcactgc ggatggccgc
     3361 cttgagcatc cccttggcgt cccgagggtt ggacggcatg gccacacgca gaccgggcat
     3421 gtgcatgaac caggcctcca ggtactggga gtggggcgcg gcgtttccca gctttccgcc
     3481 gttgggaagc cgcagcacca tgggcagtcg cactcgcccc cgggtcatgt agtgcatctt
     3541 ggacatgtgg ttggccaacg ggtccatgta tccgaggaac tccccgtact gcatctccac
     3601 caccggccgc atgccggcca gcgccgcccc gatgccggcc cccaggatgc cggcctcgga
     3661 gatgggcatg tcgatgaccc gcttgcaccc gaaacgcttg aagaggccgt ccgtggcgtt
     3721 gaaggcgccg cccatgaccc cgacgtcttc gcccaatacg aacacgcgtt cgtcccgctc
     3781 catctcctcc gcctgggcct cggagattgc ctgcgccatg ctcatcatgc tcatcccacg
     3841 gcctcctggc tctcgacgta gacgaacctc tggatgtcct ctcgagtgcc ttcgggtgcc
     3901 ggactggtct tggcgaactg cacggcctcc tcgatcacct ggcggatctc ctgcttccag
     3961 gagccgtcct ccgtgtctgc cagggcgccg tgggacttca ggtactccgc cagcttcggg
     4021 atagggtcat gggtcatcca ggcctcgttc tcttccttgg tgcggtaccc cgaggtgtcc
     4081 cagaccgtgt gcccgtgcca tcggtaacac gtggcctcca ggaagctggg cccgtccccg
     4141 tttctggccc gagagacaaa ctcgtccatg gcctgcagaa cctccacggc gtccgtacca
     4201 tccacggtgc ggcccggcat cccgtaggcg accgcgcggt ccgagatccg ccggaccggg
     4261 agcaccccct ggatgggggt cgagaccgcc atgccgttgt tctcgcacac aaagaccacg
     4321 ggcgccttgt ggatggcggc gatgctcatg ccctcgtggc agccccccgc gttcgtggcg
     4381 ccgtccccga acgcgcagcc caccacttcg gagcccttct gcatcgtgat ggccatgccg
     4441 atccccg
//
LOCUS       DASRZS010000392         3027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_603579, whole genome shotgun sequence.
ACCESSION   DASRZS010000392 DASRZS010000000
VERSION     DASRZS010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603579"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VFH83_09560"
     CDS             <1..725
                     /locus_tag="VFH83_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HET6486654.1"
                     /translation="FVGYQKGLPPGLVDLPRTIGQVVDGKPDAITMHKGPALSIWPRY
                     AGKLPLIIQSVDFVVDDSLIQVMVSVEEALRMGADALAVAIAVRGPTEGKFLKILATA
                     VEEAARWDFPIIAHIYPRDFSAQPTIVHDPENIAWAVRCGIECGADVIKVPFTGDPKS
                     YGQIVASCPVPLVAAGGPKAETLEDALGQMKGVVDSGARGATIGRNIWGFPKVTSAVK
                     AFLAVIHDGASPKGAMTSAGLA"
     gene            complement(766..1746)
                     /locus_tag="VFH83_09565"
     CDS             complement(766..1746)
                     /locus_tag="VFH83_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6486655.1"
                     /translation="MPPQARRIGNTDLLTFPLALGVGGLGIGMTEADAFELLDTYAEA
                     GGNTLDTARVYSDWVPGELHRSERILGDWLRARRNRALMIVCTKGGHPDLATMDVPRL
                     DAASLRRDVEGSLKSLRVDTIDLYWLHRDDPTRGVGEIVETLAGFVREGKIRHYGFSN
                     WSAARIREAVRHCGAHGAPAPVGDQSLLNIGCRHMGPLADPTMVVSSRDLDSVDSETG
                     LTAFAYSSQANGFFAKLSKAAAEGGQVPGSSPYDTPANRILFTTIDRHAHALDLSITQ
                     VVLGFLLTRRHPTIPIVGPRTMEQLKDTLRAADVRLPSEVFEDLRVQAGF"
     gene            complement(1756..2694)
                     /locus_tag="VFH83_09570"
     CDS             complement(1756..2694)
                     /locus_tag="VFH83_09570"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6486656.1"
                     /translation="MMRAIAAGGLTYKGRSFDTDGGAIGELRVSTDIQDDPRALRMRM
                     AEDGYLFLPGILHRREVLEARDEILRRLEAAGFLDRRYPRSEAIPVPDCPDGFVPQLA
                     RDNPELERVIFQGPMMDFYARFLGGPVRHFDFVWLRAKRPGFTDPTPPHYDVVFMGRG
                     TKQLYTSWTPLEDVPVEKGGLMILEGSHRLEEIKSTYGELDVDTYCMNRSEAPVIQSG
                     EKQWEDRVNGGQFSDDAPGLRDRLGGRWLTADYRAGDLLLFGMYLMHASGDNGSRGFR
                     LSTDTRYQLASEPVDERWIGENPVAHGPGGKRGLIC"
     gene            complement(2716..>3027)
                     /locus_tag="VFH83_09575"
     CDS             complement(2716..>3027)
                     /locus_tag="VFH83_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486657.1"
                     /translation="KAFVLSFSYALSLELRGCGVSVSVLCPGPTRTEFASRAGTESAR
                     LHRMGVMEARAVAQAAVRGLLRGKRMIVPGWMNRITAVLPRFAPRGLVAGVAMSMNSD
                     R"
BASE COUNT          517 a         1027 c          994 g          489 t
ORIGIN      
        1 acttcgtcgg ctaccagaag ggactcccgc ccggcctggt agatctcccc cggaccatcg
       61 gccaggtggt ggacgggaaa ccggatgcca tcaccatgca caagggcccg gcgctctcga
      121 tctggccgcg gtacgcgggt aagctgcctc tcatcattca gtcggtggac ttcgtggtgg
      181 acgattcgct gatccaggtc atggtcagcg tagaggaggc gcttcggatg ggggcggacg
      241 cccttgccgt tgccatcgcg gttcgcgggc ccaccgaggg gaagttcctg aagatcctgg
      301 cgaccgccgt ggaggaggct gcccggtggg acttcccgat catcgcccac atctacccca
      361 gagacttctc ggcccagccg accatcgtcc atgatcccga gaacatcgcc tgggcggtgc
      421 gctgcggaat cgagtgcggc gcggatgtca tcaaggtgcc ttttaccggg gaccccaagt
      481 cctacggcca gatcgtggcc agctgtccgg tcccgcttgt cgcggcgggt ggtcccaagg
      541 cggagacgct ggaagacgcc ctcgggcaga tgaagggcgt cgtcgacagc ggcgctcggg
      601 gagccaccat cggccgcaac atctggggct tccccaaggt gacgtcggcc gtcaaggcgt
      661 tcctggccgt catccacgac ggcgcctccc cgaaaggcgc catgacgtcc gcgggtctcg
      721 cctagcgcat caacggcgcc ggaagggtgc tcccgacagg acctgctaga acccggcctg
      781 aacccgaagg tcctcgaaga cctctgaggg gaggcgcacg tccgcggcgc ggagcgtgtc
      841 cttgagctgc tccatcgtcc tgggcccgac aatggggatc gtcgggtgtc tgcgggtgag
      901 gagaaacccg agcacgacct gggtgatgga taggtccagc gcatgcgcat gccggtcgat
      961 ggtcgtgaac aggatccggt ttgccggcgt gtcatacgga gacgagcccg gtacctgccc
     1021 cccttccgcc gcagccttcg atagctttgc gaagaagccg ttcgcctgtg acgaataagc
     1081 gaacgccgtg agccctgtct cggagtcgac ggagtccaga tcccgggagc tcacgaccat
     1141 cgttgggtcc gcaaggggac ccatgtgccg gcagccgata ttgagcaggg actggtcacc
     1201 caccggggcc ggcgccccgt gcgctccgca atggcgtact gcctctcgaa tccgggcggc
     1261 gctccagttt gaaaatccat agtgccggat cttcccctcc cggacaaagc ccgccagggt
     1321 ctccacgatc tcaccgacgc cgcgcgtcgg gtcgtcacgg tgaagccagt acaggtcgat
     1381 cgtgtcgacc cgcagggact tcaggcttcc ctcgacgtcc cggcgaagcg acgccgcgtc
     1441 caggcgaggc acgtccatcg tcgccagatc cggatgaccg cccttggtgc agacgatcat
     1501 caaggcgcgg ttgcggcgcg cgcgcagcca gtcgccgagg atgcgctcac ttcggtggag
     1561 ctccccgggc acccagtcgg agtacacccg cgccgtgtcc agcgtgttgc cgccagcctc
     1621 ggcgtaggtg tccaggagct cgaatgcgtc tgcctcggtc atcccgatgc ccagtccgcc
     1681 cacccccaag gccagaggga acgtgagaag gtcggtgttg ccgatacggc gcgcttgcgg
     1741 cggcacgcgc ggatcttagc agatcaggcc gcgcttgccg cctgggccgt gcgccaccgg
     1801 attctccccg atccatcgct cgtccaccgg ctccgaggcg agctggtagc gagtgtccgt
     1861 ggacagccga aaccccctgc tcccgttgtc gccgctggcg tgcatgaggt acatgccgaa
     1921 caggagcaga tctccggcac gatagtccgc cgtgagccat cggccgccca gccggtcccg
     1981 aagcccggga gcgtcgtcgg agaactggcc gccgttgacc cggtcttccc actgcttctc
     2041 accggactgg atgacagggg cctccgaccg attcatacag taggtgtcga cgtccagctc
     2101 gccgtaggtg gacttgatct cctccagtcg atgggatccc tccaggatca tgaggccgcc
     2161 cttctccacg ggaacgtcct ccagcggcgt ccaggaggta tagagctgct tggtcccgcg
     2221 tcccatgaac acgacgtcgt agtggggcgg cgtgggatcg gtgaatccag ggcgcttcgc
     2281 ccgaagccac acgaagtcga agtgccgcac cggtcccccc aggaaacgcg cgtagaagtc
     2341 catcatgggc ccctggaaga tcacgcgctc cagctcgggg ttgtccctgg caagctgagg
     2401 tacaaaaccg tccgggcagt cgggaaccgg gatggcctcg gaacgagggt accgtcggtc
     2461 caggaaaccg gcagcctcca gtcgccgcag gatttcatcc cgcgcctcca ggacctcgcg
     2521 gcgatggaga atcccgggca ggaagagata cccgtcctcg gccatccgca tgcggagtgc
     2581 cctcgggtca tcctggatgt ccgtggagac ccgcagctcg cctatggcgc cgccgtccgt
     2641 atcgaacgag cgacccttgt acgtgaggcc gcctgccgca atcgcccgca tcatttcccc
     2701 cttgtcccga cagtcctatc gatcggagtt catggacatg gccacgccgg caacgaggcc
     2761 ccgcggagcg aaccgcggga gcacggccgt gatccggttc atccatccgg gaacgatcat
     2821 ccgtttcccg cgcaagagac cgcggaccgc ggcctgcgct acggctcggg cctccatgac
     2881 tcccatgcgg tggagccggg cgctctcggt ccccgcccgg gaggcgaact cggtccgcgt
     2941 ggggccggga cacaggacgg agacgctcac gccgcaaccg cgcagctcca gggagagcgc
     3001 gtaggagaag gaaaggacga acgcctt
//
LOCUS       DASRZS010000393         4316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2526680, whole genome shotgun sequence.
ACCESSION   DASRZS010000393 DASRZS010000000
VERSION     DASRZS010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2526680"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="VFH83_09580"
     CDS             complement(<1..1093)
                     /locus_tag="VFH83_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486658.1"
                     /translation="MPGDHGIPNRLKALLTDLLDEHVPDPARALRELARALTPADQGV
                     VLRRVGGRVRSASGLSTDGAVRAICVMHSSGVPVPRIVRFLADNLHGPLSLAQFELAV
                     RFIFDNDPPLPQELVFDLTQYTYLESALLARRGAELSARLKDSLTPSVLLYLYLLSLR
                     DDLSNQNVHLILFVLRHCLPTLKIGQPRRAASPPEREVTEAWRFAQKTARYADVFVTP
                     EPVPRSQQPEQEDSAGPTGDAPQAGQASDSTDALVVHGLSFRRQSHRGGEPRLPEPVD
                     AGPPAPPATERSGTPRQAPAAPPDAGRRPAPSGRKTSAAVVRAAAPAKVSRETRTDRR
                     APVRETPRPEALVLKEPSQERLARSTRKPV"
     gene            1278..2123
                     /locus_tag="VFH83_09585"
     CDS             1278..2123
                     /locus_tag="VFH83_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486659.1"
                     /translation="MQGSRRLTKITRYSKEGKRRSVARAYRARLRGALLSLARTVMFC
                     ATPEQVDAAAALAQLVCSQGITSANYLLFLTLLETNNVFVVDVLVGDRNPFLLFAPIK
                     PNWYLLRETFRILAKYKGHELSEKALLALLGVVQNAYKTSKDGYKIYPLSVADVYSLG
                     KHLDKDGGQGEPRNQLLLDILYDIYFVAIDSEDRGVIRIGIKANDIRMAFFDHTKRMV
                     DALPDVLLVQDVPRLAVGPRSFSRTATPESRPLPPASQPTRPVSRSRKPSDGPGKAGR
                     TKRRP"
     gene            2270..2974
                     /locus_tag="VFH83_09590"
     CDS             2270..2974
                     /locus_tag="VFH83_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486660.1"
                     /translation="MLQFPLLRFWKEEDLYKLQVQAVTRFLKCDTAPAVEASLEFARF
                     LNAEGLTSDNYPLFLELLREENGHVIQALIGKEKAFDLFTRVQPNPYIVTFCFQMLRA
                     YKPGGVNDVTLELIYGILYRAYHSAKEGNALYRLSIEDINAVGKFLDKEKPQTESINR
                     FILDILGDIAEYRSINNEDPALDKNSSHAIAIRNAFFDRKRTMTGVMPAEILVRGDHL
                     GTTVKPRRQKPFANRT"
     gene            2986..3678
                     /locus_tag="VFH83_09595"
     CDS             2986..3678
                     /locus_tag="VFH83_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486661.1"
                     /translation="MAQMDEFNKEVWSEQSIFRVENHIINNIFACRTTDAVEAAITYA
                     GFLVRSGLTNENYPLFQKLLEVENHFVIDSLLGRTDPFLFLTPIQPTKHLVSTCFRLL
                     TNWHPGGIYPKTLAIVLGALQAAYSYAKDGYRIHTVTINDVNNLGKHLNKEKGQDEPV
                     NRAILDILDRLASLEGQGDAEMELLARQCNVIRGNFFDKRRKMEDAIPQVLLVKSDYL
                     VKEVLPATVFED"
     gene            3700..>4316
                     /locus_tag="VFH83_09600"
     CDS             3700..>4316
                     /locus_tag="VFH83_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486662.1"
                     /translation="MVILDRTKTHTATDIAAIENKMLGGILACRTTEAVEAAITYAHF
                     LNKTGLTPENYPIFIKVLEIGNHWVVDALIGSRDPFLFFSSIQPNTQLLTAAFNLLAE
                     RHPGGLYAKSLSVVLGILQAAYNVPVDGYKIYPVTVSDLNALGKHLDEEAGQEDSLNR
                     CILDILEKAALLEGMDTSNRDMEEVAIHANEIRNFFFDSTKHLNEV"
BASE COUNT          866 a         1362 c         1389 g          699 t
ORIGIN      
        1 gcaccggctt ccgggtggac cgcgcgagac gctcctgcga aggttccttc agcaccaacg
       61 cctccggtct cggcgtctca cggaccggcg cacgccgatc ggtcctggtc tcacgggaga
      121 ccttcgccgg tgcggccgcc cggaccacgg cggcggaagt ctttctgccg gagggtgcgg
      181 gccgacgccc ggcatcgggc ggagctgccg gcgcctggcg aggagttccc gagcgctcag
      241 tcgcgggcgg cgccggcggt ccggcgtcga ccggctccgg caggcgtggc tcacctccgc
      301 ggtggctctg ccggcggaag gaaaggccat ggacgacgag cgcgtccgtg ctatcggagg
      361 cctggccggc ctgcggagcg tccccggtcg gacctgcgga gtcttcctgc tccggctgct
      421 ggctacgggg tacgggctcg ggggtcacga agacatcggc atatcgagcc gtcttctggg
      481 cgaacctcca cgcttccgtc acttcccgct cgggaggcga agcggcgcga cggggctgcc
      541 caatcttgag ggtcggcagg cagtggcgca ggacgaacag gatgagatgg acgttctggt
      601 tgctgaggtc atcccgaagg gagagcaggt acagatagag gagaaccgac ggggtgaggg
      661 agtccttcag ccgcgccgag agctcggcgc cgcgtctggc caggagcgcg ctctccaggt
      721 acgtgtactg cgtcaggtcg aagacgagct cctggggcag gggagggtcg ttgtcgaata
      781 tgaaccggac ggcgagctcg aactgggcga ggctcagcgg gccatgcagg ttgtcggcca
      841 gaaaacgcac gatgcgcgga acggggacgc cgctcgagtg catcacgcaa atggcccgga
      901 ccgctccgtc cgtagagagc cccgaagcgc ttctcacgcg gccgccgacg cgccgcagga
      961 ccactccctg gtcggccggg gtcagggcgc gcgcgagctc tctgagagcg cgagccggat
     1021 ccggcacgtg ctcgtccagg agatcggtga gaagcgcctt gagccgattc gggatgccat
     1081 ggtcgcccgg catggaatgc agtcctggag gcggcgtaaa gccccgcctt ggaggcaact
     1141 gatgtagaaa gtctagtatt cctggatagc gctgtcaagt ttctggcctg tcgccgtttg
     1201 ccttccggcc gcgggcggaa cgtaaggatt atccacccgc gggccgagaa tatcgacgaa
     1261 ggaccactcg gcacaggatg caaggatcac ggagactcac caagatcacg cggtacagca
     1321 aggagggcaa gcggcgctcc gttgcccgtg cgtatcgcgc gaggctgcgc ggcgcgctgc
     1381 tctctctggc gcgcacggtg atgttctgcg cgacgcccga gcaggtcgac gccgcggcgg
     1441 cgctggcgca gctcgtctgc tcccagggga tcaccagcgc caactacctg ctcttcctca
     1501 cccttctgga gacgaacaac gtgttcgtcg tcgacgttct ggtgggcgac cgaaacccct
     1561 tcctgctctt cgccccgatc aaacccaact ggtatctcct gcgggagacc ttccgcatcc
     1621 tcgccaagta caaggggcac gagctgtcgg agaaggccct gctcgcgctc ctgggcgtgg
     1681 tgcagaacgc ctacaagacc tccaaggacg gctacaagat ctacccgctg agcgtcgcgg
     1741 acgtgtacag cctgggcaag catctcgaca aggacggggg acagggcgag ccgcgcaacc
     1801 agctcctcct ggacattctg tacgacatct atttcgtcgc tatcgacagt gaagaccgcg
     1861 gggtgattcg gatcgggatc aaggccaacg acatccgcat ggcgttcttc gaccacacga
     1921 agcggatggt ggacgctctg ccggacgtgc tcctggtcca ggacgtgccg cgcctggccg
     1981 tgggacccag aagcttctct cggaccgcga ccccggaatc caggcccctc ccgccggcgt
     2041 cccagcccac gcgcccggta tcccggagcc gaaagccgtc ggatggcccc gggaaggccg
     2101 gccggaccaa acgtcgcccc tgatttcgaa aaaaacatgg acaatctgga gattggggaa
     2161 tagacttcaa ggtgaaagct gggagttagc cacgtcttcc tcggggttcc tgcctctgca
     2221 agggccaagg ggacgatcgt gatggcgacg cggttcggac ggaggcaaga tgctccaatt
     2281 ccccctgttg aggttctgga aggaagagga cctctacaag ctccaggttc aggccgtcac
     2341 gagattcctg aagtgcgaca ccgcacccgc ggtggaggca tccctggagt tcgcccggtt
     2401 cctgaacgcc gagggcctga cctcggacaa ctatccactc ttcctggagc tgctccggga
     2461 ggagaacggg cacgtgatcc aggcactgat cgggaaagag aaggcgttcg accttttcac
     2521 ccgggtccag cctaacccgt atatcgtcac cttctgcttc cagatgctgc gcgcatacaa
     2581 gccgggcggg gtcaacgacg tgaccctgga gctgatctac gggatcctgt atcgggcgta
     2641 tcacagcgcc aaggagggaa acgcgctgta tcggctctcc atcgaggaca tcaacgccgt
     2701 gggcaagttc ctcgacaaag agaagccgca gacggaatcg atcaaccggt tcatcctgga
     2761 catcctgggg gacatcgccg aataccggtc gatcaacaac gaggatcccg cgctggacaa
     2821 gaactcctcg cacgccatcg cgatccgcaa cgcgttcttc gaccgcaagc gaacgatgac
     2881 gggcgtcatg ccggcggaga tcctggtacg cggagatcac ctcggaacga ccgtgaaacc
     2941 gagacgacag aaaccctttg cgaataggac ataggaggcg agaacatggc tcagatggat
     3001 gagttcaaca aggaggtgtg gtccgaacag tcgatcttcc gggtcgagaa ccacatcatc
     3061 aacaacatct tcgcatgccg gaccacggac gcggtggagg cggcgatcac gtacgcgggg
     3121 ttcctcgtga ggagcggtct gacgaacgag aactatccgc tgttccagaa gctcctggaa
     3181 gtggagaacc acttcgtgat cgactccctg ctgggcagga cggacccgtt cctgttcctg
     3241 acgcccatcc agcctaccaa gcacctggtg tccacctgct tccggctgct gaccaactgg
     3301 caccccggcg ggatctaccc caagaccctg gccatcgtcc tgggcgccct ccaggcggca
     3361 tacagctacg ccaaggacgg ctaccggatc catacggtga ccatcaacga cgtgaacaac
     3421 ctgggcaagc atctcaacaa ggagaagggc caggacgagc cggtcaaccg ggccatcctg
     3481 gacatcctgg accgccttgc ctctctggag ggtcaagggg acgcggagat ggagctcctg
     3541 gcgcgccagt gcaacgtcat ccgagggaac ttcttcgaca agcggaggaa gatggaggat
     3601 gccatccccc aggtcctcct ggtcaaatcg gactacctgg tgaaggaagt cctgccggcc
     3661 accgtgttcg aagactagac gcggacaagg aggaaaagca tggtcatcct ggacaggaca
     3721 aagacccata ccgccacgga tatcgcggca atcgagaaca agatgctggg cgggatcctg
     3781 gcctgccgta ccacggaagc cgtggaggca gccatcacct acgcccactt tctcaacaag
     3841 accgggctca cgcccgagaa ctacccgatc ttcatcaagg tgctggagat cggcaatcac
     3901 tgggtggtcg acgccctgat cggatcgcgg gatccgttcc tgttcttcag ctccatccag
     3961 cccaacacgc agctcctgac ggcggcgttc aacctcctgg ccgagcggca tcctggcggg
     4021 ctgtacgcca agtccctctc ggtggtgctg ggcatcctgc aggcggccta caacgtgccc
     4081 gtggacggct acaagatcta tccggtcacg gtctcggacc tcaacgccct gggcaagcac
     4141 ctggacgagg aagccggaca ggaagactcc ctgaaccgct gcatcctgga tatcctcgag
     4201 aaggcggcgc tgttggaagg catggacacc agcaaccggg acatggagga agtggcgatc
     4261 cacgcgaacg agatccgcaa cttcttcttc gacagcacga agcacctgaa cgaggt
//
LOCUS       DASRZS010000394         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2909928, whole genome shotgun sequence.
ACCESSION   DASRZS010000394 DASRZS010000000
VERSION     DASRZS010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2909928"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFH83_09605"
     CDS             <1..214
                     /locus_tag="VFH83_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HET6486663.1"
                     /translation="ETGITAANWKSFEKRYRHELDAPDNARLLDFLALLSRSTNFAVG
                     CYCEEESRCHRSILRQVLQARGAALR"
     gene            211..549
                     /locus_tag="VFH83_09610"
     CDS             211..549
                     /locus_tag="VFH83_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HET6486664.1"
                     /translation="MSSRTAGRHPVRRLGREKRTVAAMIALYCRARHGSTGALCSSCE
                     ELRTYAELRIDRCRFHAAKPVCARCAVHCYSSERRERIREVMRFSGPRMMLHHPLLAL
                     LHGVDSLRRP"
     gene            549..1310
                     /locus_tag="VFH83_09615"
     CDS             549..1310
                     /locus_tag="VFH83_09615"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET6486665.1"
                     /translation="MLVDAHAHCDRYGDRTDQAIAQMNHYRILTLGVAMDIPSYQRTV
                     ELAARSPYLKATFGIHPWEARTYCDRLSELDPYLRRTPLIGEAGLDFFWVEDQTTFPC
                     QRKVFEHQCQWAARTGKIMNLHTKGAETDVLQAIRELRLKSPIIHWYSGPLGLIDQFL
                     SCGCYFTVGVETLVSEEARQIARAIPLERLLLETDNPGGYEWLTRIVGMPRLIRDVLT
                     AVAETRGIEPAELEARLTGSWTALTSAIPELAGPL"
     gene            1362..1913
                     /locus_tag="VFH83_09620"
     CDS             1362..1913
                     /locus_tag="VFH83_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018102866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET6486666.1"
                     /translation="MDYIALLRGVNVGGHVVKMTDLRALLQALGLQDVRTYIQSGNVF
                     FSSDEADREALAARIEGRLEKSLGYEVPTCLRTTQEVRKLVTRNPFDGLTVTPEMRLC
                     VVFCRDPIPKGLALPLLSPKKDMEILSATKQEVFVVWHVINGRPPSSAFLDQSLGKRT
                     TSRFYHTLAKILEAAGTKAKEED"
BASE COUNT          347 a          664 c          689 g          349 t
ORIGIN      
        1 cgagacgggg atcacggcgg cgaactggaa gtccttcgag aagcgctatc ggcacgagct
       61 cgacgcgccg gacaacgcgc gcctcctgga cttcctggcg ctgctctcca ggagcacgaa
      121 cttcgccgta ggctgctact gcgaggaaga gagccgatgc catcgctcga tcctgagaca
      181 ggtgctccag gcgcgggggg ctgctctccg gtgagcagcc gtacggcggg ccgccacccc
      241 gtcagacgtc tgggtcgcga gaagcgcacg gtggcggcga tgatcgccct gtactgccga
      301 gcgcgccacg gttccacggg cgctctctgc tcctcctgtg aggagcttcg cacgtacgcc
      361 gagctcagga tcgaccgatg ccggttccac gcggcgaagc cggtctgcgc gcgctgtgcg
      421 gtccactgct actcttccga gcgccgggag cgcatccggg aggtcatgcg cttctcggga
      481 ccccgcatga tgctccacca tccgctgctc gcgttgctcc acggggtgga cagcctccgg
      541 cggccgtgat gctcgtcgac gcgcacgccc actgcgaccg ctacggcgat cgcacggacc
      601 aggccatcgc ccagatgaac cactaccgga tcctgacgtt gggcgtggca atggacatac
      661 cctcctatca gaggaccgtg gagctggcgg cgcgctcacc gtatctgaag gcgaccttcg
      721 ggattcaccc gtgggaggcg cgcacctact gcgatcgcct gagcgagctg gatccgtact
      781 tgagacggac ccccctcatt ggggaggccg ggctggactt cttctgggtc gaggaccaga
      841 cgaccttccc gtgtcagcgc aaggtgttcg agcaccagtg ccagtgggcc gcgcggacgg
      901 gcaagatcat gaacctgcac accaagggcg ccgagacgga cgtgctccag gccatccggg
      961 agctgcggct gaagagcccc atcatccact ggtactcggg tccgctgggg ttgatcgacc
     1021 agttcctttc ctgcggctgt tacttcacgg tcggcgtgga gacgctggtc tccgaggaag
     1081 cccgacagat cgcgcgggcc atccctttgg agcggctgct gctggagacc gacaacccgg
     1141 gtggatacga atggctcacc aggatcgtcg ggatgccccg gctgatccgg gacgtgctga
     1201 cggccgtggc tgagaccagg ggaatcgagc cggccgagct ggaggcgcgg ttgacgggct
     1261 cctggaccgc tctgacctcc gccatccccg agcttgccgg tccgctctga tcccaggcgt
     1321 cttggtggac gtgggcgtcg gcttctgggt agcttgagcg gatggattac attgccctcc
     1381 tgcgtggcgt caacgtcggc ggccatgtcg tcaagatgac ggacctccgc gccctgctgc
     1441 aggcgctggg cctccaggac gtccgcacct acatccagag cggcaacgtg tttttctcct
     1501 ccgacgaagc cgatcgcgag gcgctggccg cccggatcga aggccgcctg gagaaaagcc
     1561 tcggctacga ggtgcccacg tgtcttcgga caactcagga ggtccgaaag ctcgtgacca
     1621 ggaacccgtt cgacggtctg acggtcacgc cggagatgag gctgtgcgtg gtcttctgcc
     1681 gggacccgat ccccaagggc ctggccctgc ccctgctgtc gcccaagaag gacatggaga
     1741 tcctgagcgc cacgaagcag gaggtctttg tggtctggca cgtgatcaac ggcagaccac
     1801 cgagctccgc gttcctggat cagagcctcg gcaagagaac gaccagccgg ttctatcaca
     1861 cccttgccaa gatcctggag gccgccggga cgaaggcgaa ggaggaggat tgatgccgga
     1921 gatcttgagc ctggatgcgg atgcggtccg ctgccgtgag ccgctggttg ggcgctacct
     1981 cacgatgctg gaggactgcc gatcgctgac gctggatagc atcaagaacg tgcgcggtga
     2041 caccctgta
//
LOCUS       DASRZS010000395         3403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_989077, whole genome shotgun sequence.
ACCESSION   DASRZS010000395 DASRZS010000000
VERSION     DASRZS010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989077"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1554)
                     /locus_tag="VFH83_09625"
     CDS             complement(<1..1554)
                     /locus_tag="VFH83_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486667.1"
                     /translation="MGIILKYIITSIRERKFRTFLILFAITLSTALFFASSALSLNLQ
                     DMYMEVMKKYYGSSNLMVAQGRGAAWNLSMARAEKYGDRLSYLIGTIDGSATYRPNRT
                     ESVSFYLRGFRIEDLPVFASFTLAEEAELDPFTGAKVIVSQIAAKRYGWAVGDTITLD
                     MNDVKRHFVLVGIALPTGVFQEGFDRNVTGVVPRDYLAGLYDIRGRVTTAYLQTKSPG
                     EVAPLLIELAADYPRYSVREPVDKAQRESFASDIKTPLMLMTIMVLIMSVFIIYSSFK
                     VITVERMPILGTFRSIGATRRTTDGVLMLETFLYGAVGGICGCLLGIGILSLMTNLLA
                     YNAWAGIRMKTELHFETIHLVLAFALAVGLSFVSCLIPIVRVSRIPVKDIVLNTMDHG
                     VKARPVRFIAACVVLAVAIVVPRVVPRSLAFVADAGCLFLVIAALIALVPFLTTALVA
                     VLQRVQQLAFGNEGALAAKNLRENRNVLNNISLLGISICSLILIFSISFSAIQQTIGF
                     YTDARFELWA"
     gene            1713..2909
                     /locus_tag="VFH83_09630"
     CDS             1713..2909
                     /locus_tag="VFH83_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET6486668.1"
                     /translation="MLGVRGDPESARFINRSLVLGSMKSGEVLSRAELARQLRLSKVT
                     ISQVVADLISEGLVEETGDGGTSSTGGRRPRLIRVVSSGPYVIGLDIGLTNTVVALAG
                     LDGTRIEQFREPTDVKHDAGSILAQIETLTEAILAKRNVPRTMILGVGISIGGLIDGG
                     TGYIAFSPDFGWEELPFRGMVEQRLGLPTILDNCTRAMALGENWHEKSYDTRNIFYVN
                     VGHGIGSAIVMHGRIYNNNSEFGHIRITNRDVLCDCGKRGCLEAVASGHAIEKLAAAA
                     QDAQVSAARSAKEVAMLASAGNATALGIFRDASRYLGRALSIAATLFNPDRVVIAGGV
                     ASARHLLEEPLMDEYTATTMDVIKNSTVVTFSSYGMDAGINGAICLALDRFVFHGEAA
                     IVANST"
     gene            3032..>3403
                     /locus_tag="VFH83_09635"
     CDS             3032..>3403
                     /locus_tag="VFH83_09635"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET6486669.1"
                     /translation="MASFTYGQPTEIWFGRGRIQETGKAAWSLGKRCLLVTGPVSDAR
                     RPQIDAVTSSLRAAGLAVAHFDGVVPNPTTESITAGAGVAREHKADVVVGLGGGSSMD
                     SAKAIAVESTHEGSAWDYLFFK"
BASE COUNT          665 a         1021 c         1132 g          585 t
ORIGIN      
        1 ggcccacagc tcgaaccggg cgtcggtata gaagccgatg gtctgttgga tcgccgagaa
       61 gctgatcgag aagatgagga tcaggctgca gatggaaatc ccgaggaggg agatgttgtt
      121 gaggacgttg cggttctccc gaaggttctt tgccgcaagg gctccttcgt tcccgaacgc
      181 gagctgctgc acccgctgga gaaccgccac cagggccgta gtgaggaacg ggacgagcgc
      241 gatgagggcc gcgatgacca ggaagagaca cccggcgtcc gccacgaagg ccaggctcct
      301 ggggacgacc cgagggacca cgatcgccac ggccaggacc acgcaggcgg cgatgaaccg
      361 gacgggccgc gctttcacgc cgtgatccat ggtgttcagc acgatgtcct tcacggggat
      421 ccgggagact cgaacgatgg ggatcaggca gctcacgaag gacaatccca ccgccagggc
      481 gaacgccaga acgaggtgga tggtctcgaa gtggagctcg gtcttcatgc ggatgccggc
      541 ccaggcgttg tacgccaaca ggttggtcat gagggaaagg atgccgatcc ccaagaggca
      601 cccgcagatg cccccgacgg cgccatagag gaacgtctcc agcatcagca cgccgtccgt
      661 ggtcctccgc gtggcgccga tgctgcggaa ggtcccgagg atgggcatcc gttccacggt
      721 gatcaccttg aacgagctgt agatgatgaa gacgctcatg atcaggacca tgatcgtcat
      781 gagcatcagg ggggtcttga tgtcgctcgc aaagctctct cgctgggcct tgtccacggg
      841 ctctctcacc gagtagcggg gatagtccgc cgccagctct atcagaagag gcgccacctc
      901 ccccgggctc ttcgtctgca ggtacgccgt tgtcactctg ccgcggatgt cgtagagccc
      961 cgccaggtag tctcggggga cgaccccggt gacgttgcga tcgaagccct cctgaaatac
     1021 tccggtcggc agcgcgatcc cgacaagaac gaagtgcctc ttcacgtcgt tcatgtccag
     1081 ggtgatggta tccccgaccg cccagccgta gcgcttcgcc gcgatctggg agacgatcac
     1141 cttggcgccc gtgaacggat ccagctccgc ctcctccgcg agcgtgaacg atgcgaagac
     1201 gggaaggtcc tcgatccgga atccccgcag gtagaagctg acgctctccg tccggttggg
     1261 ccggtaggtg gcgcttccgt caatcgtgcc gatcaggtag ctcagccggt ctccgtactt
     1321 ctccgcccgc gccatggaga ggttccaggc tgctccgcgc ccctgggcga ccatgagatt
     1381 ggaggacccg tagtacttct tcatcacctc catgtacatg tcctggaggt tcaacgagag
     1441 cgcgctggag gcgaagaaca gagcggtgga aagggttatg gcgaaaagga tgaggaaggt
     1501 ccggaacttg cgttcgcgga tgctcgtgat gatgtacttc aggataatgc ccatgccgac
     1561 ggcgacctgt tctacttgaa ccccattgga cccgagatgt gcccgcgcgg caacccgcat
     1621 aggcgggcgt aagtaggatc cttgacgtcg aggccttcgg aagcgtgtct gcttcctctt
     1681 gaccacctta gtaagtatgc tatactaacg atatgcttgg cgttcggggg gatccggaga
     1741 gcgcgaggtt catcaaccgg tcactggtgc tgggcagcat gaaatccggt gaagtgcttt
     1801 cccgcgccga gcttgcccgt cagttgcgtc tgagcaaggt cacgatttcc caggtcgttg
     1861 cagacctcat ctcggaaggc ctggtcgaag agacgggcga tggcgggacg agcagcaccg
     1921 gcgggcgaag accgaggctc attcgcgtcg tctcgtcggg cccctatgtg atcggactcg
     1981 acatcggact caccaacacc gtggtggcgc tggccgggct cgatggaacg aggatcgagc
     2041 agttccgcga gcccacggat gtgaagcacg acgccggaag catcctcgcg cagatcgaga
     2101 ccttgacgga agccatcctc gccaagagaa acgtcccccg aacgatgatc ctgggcgtcg
     2161 gcatttccat tggagggctg atcgacggcg ggaccggcta catcgccttt tctcccgact
     2221 tcggctggga ggagttgcca ttccgcggca tggtggagca gcgcttgggc ctgccgacca
     2281 tcctcgacaa ctgcacccgc gcaatggcgt tgggcgagaa ctggcacgag aagtcgtacg
     2341 acaccaggaa catcttctac gtcaacgtgg gacacggcat cgggtccgcg atcgtcatgc
     2401 atggaaggat ctacaacaac aacagcgagt tcgggcacat ccggatcacc aaccgggacg
     2461 tgctctgtga ctgtgggaag cgaggctgtc ttgaggccgt ggcatccggg cacgccatcg
     2521 agaagctggc cgcggcagcc caggacgcgc aagtttcggc ggcgcgctcg gcgaaagagg
     2581 tggccatgtt ggcgtccgcg ggaaacgcaa cggccctggg gattttccgg gatgccagtc
     2641 gctacctcgg ccgcgcgctg tccatcgccg caacgctgtt caaccccgac cgggtggtca
     2701 tcgccggcgg tgtggcgagc gcgcggcacc tgctcgaaga acccctcatg gacgagtaca
     2761 ccgcgacgac gatggacgtc atcaagaact caacggtcgt cacgttctcc agctacggca
     2821 tggatgcggg gatcaacggc gccatctgcc tggcgctcga tcggttcgtg ttccacgggg
     2881 aggcggccat cgttgccaac tccacgtgag cccggccagt ggcaggccgg gaatcggaag
     2941 acgccagtga cggtgccccg attcccacga ctatattctg gagaggaagg actggacgtc
     3001 cggagcggtc gtcctgccca ggaggaacgg gatggctagc tttacgtatg gccagccgac
     3061 cgagatctgg ttcggccggg gacgcatcca ggagacggga aaggcggcat ggagcctcgg
     3121 gaaacgctgt ctcctggtga cgggaccggt gtccgacgcc aggaggccgc agatcgacgc
     3181 ggtgacctcc agccttcggg cggccggcct ggcggtcgcc catttcgacg gcgtggtgcc
     3241 gaacccgacc accgagagca ttacggcggg agccggagtg gcccgggagc ataaggcgga
     3301 cgtggtcgtc ggacttgggg gcgggtcaag catggatagc gcgaaggcca tcgccgtgga
     3361 atcgacccat gaaggaagcg cctgggacta cctcttcttc aag
//
LOCUS       DASRZS010000396         4113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5606476, whole genome shotgun sequence.
ACCESSION   DASRZS010000396 DASRZS010000000
VERSION     DASRZS010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5606476"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VFH83_09640"
     CDS             complement(<1..967)
                     /locus_tag="VFH83_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6486670.1"
                     /translation="MRRPPTIKDVAARAGVTVTTVSRCLNRRGYMSEATRVRIQQAMT
                     DLDYVPNEIARSLLRRRSSIIGLIIPTANHPFFGQLTTSIEARAYEAGYKLMLCDSRL
                     DSEKESQYIDMLIRHQVDGIIMASHTLRVDQYRRIGMPLVSMDRRIAERIPYVTSDNT
                     AGGRLATRRLIEQGCRRLAFFTGKVNRALLPSRRVEAFEEEARAADLEPIVVQTGEDV
                     FDFTQYQTLVDQLFREHPDVDGVIASDVKAGHVLQACRRFGRRVPQDVKVVGYDDILL
                     ASLLVPRLTTIHQPIEEMGSRAVDVLVAQIAGEAVAMKNVLPVSLV"
     gene            1134..2468
                     /locus_tag="VFH83_09645"
     CDS             1134..2468
                     /locus_tag="VFH83_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6486671.1"
                     /translation="MKRAAFLAVSLILAAPMLLLAAGSKEGSSTQGQAAPVTSIGSAP
                     VVLNAYFETGFDIPFKLAEEFTKQYPNVTWNIKQDQFTNLINETPRLLAGDNPPDLIR
                     LPTMVSFAKQGLLKNLDGYAKAYGWDAWPVPQLNQNRVAADGTRGSGALYAMGLNYSL
                     TGVFYDKKLAAQIGMTQPPRTLAEFEGLLAKAKAAGLLPIMEWGSAKSGMGLAFPLQA
                     IMASVGPVGPINDWIFQKKGATVDTPDNRNAAQHLAQWIQNGYFPPDINAIEYTDSNA
                     RFVKGEGVFTFNGDWENADYDQNMAGNVGFFLMPPAAASGSPAAMSAPLTYGIAAKAK
                     NADVAAFFLNWVATNPAARQIDVAIGGSNPGGPADATMPSVAAGSITNDTLAAGPVVG
                     KAGTSMDFIANATSSIFAQGWTPELQKMVGGKEDPAGLLKAVQAEYQRELSQ"
     gene            2633..3451
                     /locus_tag="VFH83_09650"
     CDS             2633..3451
                     /locus_tag="VFH83_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6486672.1"
                     /translation="MYAVFVLLPLLMSIRYSFLRWDGITPPTWVGLRNYGTVFQVPDL
                     IGTILNAFKLVVFFSFIPVALGLVVAAIMHRVARGRLGAVARTVLFLPQVIPLVAAGI
                     IWGWLLALPGLINQILRVVGLGAVTRAWLGDFAWALPAVGVIGIWVLLGFCTVLLLTG
                     MTNVDPGLYESARIDGAGWSREFASITVPLLRHEIGVCLTVTVIAALAAFDIVYVSTA
                     GGPGNSTAVPGIQIYILAFTEQRIGLASALAVMLMVLVLVVILPIQRLSRETAR"
     gene            3448..>4113
                     /locus_tag="VFH83_09655"
     CDS             3448..>4113
                     /locus_tag="VFH83_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6486673.1"
                     /translation="MKVAAGELAGGRTLLVALMVLTILPFLSIFTTALAPSGTVPSGL
                     GWPAHPQWGNFVEAFRVANMSALLASSTFIVIAVVPVALVISTMAAFAIGLLRIPGSR
                     ALLFLFVFGLTLPFGGIIVPLYYLERSLRIYNTRLAIVLPLIGLYMPFAVFWMRAHFV
                     NMSAEISEAARLDGANTWDLFWRVHFPLARAPISSLGILMAVWTWNQFLLALVLVEDP
                     THRT"
BASE COUNT          667 a         1358 c         1324 g          764 t
ORIGIN      
        1 ccacgaggga gaccggcagg acgttcttca tggctacggc ttctccggcg atctgcgcca
       61 ccaggacgtc taccgcccga ctccccatct cctcgatggg ctggtggatg gtcgtcagtc
      121 gagggaccag gagggatgcg agcaggatgt cgtcgtaccc gacgactttg acgtcctgcg
      181 gcaccctcct cccgaaccgc cggcacgcct gcagcacgtg gccggctttg acgtcgctgg
      241 cgatcacccc atcgacgtcg ggatgctccc gaaagagctg gtccaccagg gtctgatact
      301 gcgtgaagtc gaacacgtcc tcgcccgtct gcacgacgat cggctccaga tccgcggccc
      361 tcgcttcctc ctcgaacgcc tcgacgcggc ggctggggag aagcgcgcgg ttcaccttgc
      421 cggtgaagaa cgccaaacgc cggcatccct gctcgatcaa ccgccgggtg gccagcctcc
      481 cacccgccgt gttgtcggag gtcacgtagg ggatccgctc ggcgatccgg cgatccatcg
      541 aaacaagcgg catcccgatg cggcggtact ggtccacccg cagcgtgtgg ctggccatga
      601 tgatgccgtc cacctggtga cggatgagca tgtcgatgta ctggctctcc ttctcgctgt
      661 ccaggcggga gtcgcagagc atgagcttgt agccggcctc gtaggcccgc gcctcgatgg
      721 aggtcgtgag ctgtccgaag aacgggtggt tcgcggtggg aatgatgaga ccgatgatgc
      781 tggagcggcg ccgcaggagc gaacgggcga tctcgttcgg cacgtagtcg aggtctgtca
      841 ttgcctgctg gatccggacg cgcgtcgcct cgctcatgta gccgcgtcgg ttgagacagc
      901 gggagaccgt ggtgacggta acgcccgccc gcgccgcgac atccttgatg gtaggcggtc
      961 tgcgcatcct ggaaacgata gcgcagcgga gcgccgcggg caagcgaggc acggttcaag
     1021 gccaggaaaa gccggtatat caatcgattg acatatcggg gcgcccggcc gtaccttggg
     1081 gttagtcgtc ccgacgttcg acgacagaaa cgattctagg gaggtgcaaa cgtatgaaac
     1141 gtgcggcatt tctcgcggta tcgctgatcc tggccgcccc gatgctgctg ctcgcggctg
     1201 gatccaagga aggctcgtcc acccaaggcc aggcggcccc cgtcacgagc attggctcgg
     1261 cgccggtcgt cctgaacgcg tacttcgaga cgggattcga tatccccttc aagctggcgg
     1321 aggagttcac gaagcagtac ccgaacgtga cctggaacat caagcaggac cagttcacga
     1381 acctcatcaa cgagaccccg cgcctcctgg cgggagacaa cccgccggac ctcatccggc
     1441 tgccgacgat ggtctccttc gccaagcagg gtctgctcaa gaatcttgac ggctacgcca
     1501 aggcatacgg ctgggacgcg tggcccgttc cccagctcaa ccagaaccgc gtcgcggcgg
     1561 atggcacccg aggctccggc gctctctacg ccatgggcct caactacagt ctgacgggcg
     1621 tcttctatga caagaagctg gccgcgcaga tcggaatgac ccagccgccg aggactctcg
     1681 cggagttcga gggactcctg gcaaaggcaa aggccgcggg gctcctgccc atcatggagt
     1741 gggggagcgc caagagcggc atggggctgg cgttcccgct gcaggccatc atggcatccg
     1801 tgggcccggt aggtccgatc aacgactgga tcttccagaa gaagggcgca acggtcgaca
     1861 ccccggacaa ccggaatgcc gcgcagcacc ttgctcagtg gatccagaac ggctacttcc
     1921 ccccggacat caacgccatc gagtacaccg actccaacgc ccgcttcgtg aaaggggagg
     1981 gtgtctttac gttcaacggt gactgggaaa acgcggacta cgaccagaac atggccggca
     2041 acgtcgggtt cttcctgatg ccgccggcgg ccgccagcgg cagcccggcg gccatgtccg
     2101 cgccgctgac gtacggcatc gccgcgaaag cgaagaacgc cgatgtggcc gcgttcttcc
     2161 tcaactgggt cgccaccaac ccggcggcgc gtcagatcga tgtcgcgatc ggcgggtcga
     2221 accctggtgg accggcggat gcaacgatgc cgagcgtggc ggccggctcg atcaccaacg
     2281 atacgctggc ggccgggccg gtggtcggca aggcgggcac ctcgatggac ttcatcgcca
     2341 acgcgacgag ctccatcttc gcccagggtt ggacgcctga gcttcagaag atggtggggg
     2401 gcaaggagga tcccgctgga ctgctcaagg cggttcaggc agaataccag agggagctct
     2461 cccagtagcc cggatggccg cggaaagatt gccgcgccgg ccggcggtga caacgatggc
     2521 gacgccagcg tatccttccg cggctcccgc cccctcccgg cgggtggtcc gcaccagcgg
     2581 gttccgccgg gagacgatca tcggtggact gtttctcgca cccgccctgc tgatgtatgc
     2641 ggtgttcgtc ctgcttccgc tcctgatgag catccgttac tcgttcctcc gatgggacgg
     2701 gatcactcca ccgacctggg tcgggttgcg gaactacggg accgtcttcc aggtcccgga
     2761 cctgatcggg acgatcctca acgccttcaa gctggtggta ttcttcagct ttatcccggt
     2821 tgcgctcggt ctggtcgtcg ccgccatcat gcaccgggtc gccaggggaa gactcggcgc
     2881 ggtggccagg accgttctgt tcctcccgca ggtcattccg ctcgtcgcgg cgggaatcat
     2941 ctggggctgg ttgctggccc ttccgggctt gatcaaccag atcctccggg tggtcgggct
     3001 cggagccgtc acgcgggcct ggctgggaga cttcgcctgg gcgctgcccg ctgtcggcgt
     3061 catcggcatc tgggtgctgc tgggattctg caccgtcctg ctcttgaccg gcatgaccaa
     3121 cgtcgacccc ggtctgtacg agtccgcgcg gatcgacggg gcgggctggt cgcgggagtt
     3181 cgcctccatc accgtcccgc tcctgcggca tgagatcgga gtgtgcctca cggtcaccgt
     3241 catagcggcg ctcgcggcat tcgacatcgt ctacgtctct accgccggcg ggcccggcaa
     3301 ctcgacggct gtcccgggaa tccagatcta catcctggcg ttcaccgagc agcgcatcgg
     3361 actggcttcc gccctggctg tgatgctcat ggtcctcgtt ctggtggtga tcctccctat
     3421 ccaacggctg agtcgggaga ctgcacgatg aaggtggcag ccggcgagct cgcgggaggc
     3481 cgtaccctcc tggtggcgct catggtgttg acgatcctcc ctttcctgag catcttcacg
     3541 accgcgctgg ccccgtcggg taccgtgccc agcggcctcg gatggcccgc gcacccgcag
     3601 tggggcaact tcgtcgaggc gttccgcgtg gcgaacatgt ccgcgctcct cgcctcgagc
     3661 acgttcatcg tgatcgccgt ggtgccggtc gcgctcgtca tctcgacgat ggctgcgttc
     3721 gctatcggtc tcctgcggat cccgggctcc cgggcgctcc tcttcctgtt cgtcttcgga
     3781 ttgacgcttc ccttcggcgg aatcatcgta ccgctctact acctcgagcg atcccttcgg
     3841 atctataaca cccggttggc catcgtgctg ccgctcatcg gtctgtacat gccgttcgcc
     3901 gttttctgga tgcgcgcgca tttcgtcaac atgtcggccg agatctccga agccgctcga
     3961 ctcgacggcg cgaacacgtg ggacctcttc tggcgcgtcc acttcccgtt ggcgcgggcg
     4021 ccgatctcgt cgctggggat cctcatggcg gtctggacgt ggaaccagtt tctcctggcg
     4081 ctggtgctcg ttgaagatcc gacgcatcga acc
//
LOCUS       DASRZS010000397         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2139796, whole genome shotgun sequence.
ACCESSION   DASRZS010000397 DASRZS010000000
VERSION     DASRZS010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139796"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFH83_09660"
     CDS             complement(<1..321)
                     /locus_tag="VFH83_09660"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486674.1"
                     /translation="MSPAGPAFRLIVHPKMDGRWNMAVDEALAEAAGGGQSLPVVRLY
                     GFSPPTLSLGRFQKARGQLDAGRLASDGVVLVRRPTGGHAVLHDDELTYSVALPKQAS
                     ADGPA"
     gene            complement(318..1259)
                     /gene="trxB"
                     /locus_tag="VFH83_09665"
     CDS             complement(318..1259)
                     /gene="trxB"
                     /locus_tag="VFH83_09665"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET6486675.1"
                     /translation="MTTDRDLIIIGAGGAGLSAAQYGARGNLRTLVLEEVASGGQALV
                     ISELENYPGFPEPINGYDFTQKMEDQARKFGAEFENASVTGIQREDHVFRVTTDKGTL
                     TAPTVILATGAVHKTLGVPGEAEFSGHGVSYCATCDGPFFKAKRMLVVGGGDAACDEA
                     MYLSHLASGVLLVHRRDKFRAQKSLAARVLENPKIEVRFNTELRKIEGDKVVSRVTLY
                     NNVAKEQSEEPVAAVFVFIGSEPKSTLAASLGVPLDDIGYIKTNDRMETSVPGLYAVG
                     DVRSTPFRQLVVAASEGAIAAHAAGQYIDELKGEKYL"
     gene            1367..1870
                     /locus_tag="VFH83_09670"
     CDS             1367..1870
                     /locus_tag="VFH83_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486676.1"
                     /translation="MDERRHALVLALKDLIDREARLFTVLGEEVRKLRDTFQEKNWGP
                     SLALAENIQRSSRAVEEADTARDRAFMSLLEALDLPPETTMSAVLPALPDTLRPDMEE
                     SWRGLRMAVVRVKTSTGRMRYSAETLATALNGILEEAFPYRKGKIYSRRGTATGVSGA
                     VLVDRRL"
     gene            1880..>2294
                     /gene="flgK"
                     /locus_tag="VFH83_09675"
     CDS             1880..>2294
                     /gene="flgK"
                     /locus_tag="VFH83_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313554.1"
                     /note="with FlgL acts as a hook filament junction protein
                     to join the flagellar filament to the hook; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HET6486677.1"
                     /translation="MQSTFAPIELGKRGLVATTQGLQTVGHNLSNASVDGYSRQRVEL
                     TASPPLYEPQLNREETPGQIGQGVEVERITRIRDLLLEGRIVSEQNVQGYWEARDKYI
                     LMMEQVYNEPTDHSVRALLDKFWESWQDLSLHPSEI"
BASE COUNT          381 a          775 c          770 g          368 t
ORIGIN      
        1 ggcggggccg tccgcggaag cctgcttggg gagcgccacc gagtacgtga gctcgtcgtc
       61 gtgcagcacg gcgtggccgc cggtcggccg ccgcaccagg acgacgccgt ccgatgcaag
      121 ccgccccgcg tcgagctgcc cgcgcgcctt ctggaagcgt ccgagcgaca gggtgggggg
      181 ggagaagccg tacaggcgga ccaccggcag gctctgcccg ccgcctgccg cctcggccag
      241 ggcctcgtcc accgccatgt tccagcgccc gtccatcttc gggtgcacga tcaggcggaa
      301 cgccggaccg gcagggctca aaggtatttc tcccccttga gctcgtcgat gtactggccc
      361 gcggcgtggg ccgcgatggc cccctccgac gcggcgacca cgagctggcg gaacggggtg
      421 gaccgcacgt ccccaacggc gtacaggccg ggcacgctgg tctccatgcg gtcgttggtc
      481 ttgatgtatc cgatgtcgtc cagggggacg cccagggatg cggcgagggt gctcttgggc
      541 tcggagccga tgaacacgaa taccgcggcc accggttcct ccgactgctc cttcgcgacg
      601 ttgttgtaca gcgtcacgcg ggagaccacc ttgtccccct cgatcttcct gagctcggtg
      661 ttgaaccgga cctcgatctt cgggttttcc agcacgcggg ccgcgaggga tttctgcgct
      721 cggaacttgt cccgccggtg gaccagcagc acgccggagg cgaggtggga gaggtacatg
      781 gcctcatcgc atgccgcatc tcctccccct accacgagca tccgcttggc cttgaagaac
      841 ggcccgtcgc aggtggcgca gtaggacaca ccgtggccgg agaactccgc ctcgccgggg
      901 accccgaggg tcttgtgcac cgcgcccgtg gccaggatga ccgtgggggc cgtgagggtg
      961 cccttgtccg tggtaacccg gaacacgtgg tcttccctct ggatacccgt caccgaggcg
     1021 ttttcgaact ccgcgccgaa cttcctggcc tggtcctcca tcttctgggt gaaatcgtag
     1081 ccgttgatgg gttcagggaa cccggggtaa ttctcaagct cgctgatcac gagggcctgg
     1141 cctcccgagg ccacttcctc caggaccagg gtccgcaggt tgccgcgcgc cccgtactgg
     1201 gccgcggaaa ggcctgcgcc tcccgccccg atgatgatca ggtcccgatc cgtcgtcatg
     1261 ccatcctccg aaaactgttt agggatcaag atagcagatt gccggtctca tgggaaggaa
     1321 agttcttccg acactagtag caggatcgca cgaaggggag acgaccatgg atgagcgaag
     1381 gcatgccctg gtcctggcgc tgaaggacct catcgaccgg gaggcgcggc tgttcaccgt
     1441 gctgggagag gaagtccgca agctgcggga cacgttccag gagaagaact gggggccgag
     1501 cctggcgctc gcggagaaca tccagcgctc ctcgcgcgcc gtggaggaag ccgacacggc
     1561 ccgggaccgc gcgttcatgt cgctgctgga ggccctcgac ctgccgcccg agaccacgat
     1621 gtccgccgtg ctgccggcgc ttcccgatac gctccgcccc gacatggagg agagctggcg
     1681 cgggcttcgc atggccgtgg tgagggtgaa gacctccacc gggcggatgc gctactcggc
     1741 ggagaccctc gccaccgccc tgaacgggat cctggaggaa gccttcccgt accggaaagg
     1801 caagatctac tcgcgccgtg gcaccgccac gggcgtgagc ggcgcggtcc tggtggaccg
     1861 ccgcctgtag gaggcccacg tgcagtccac attcgcaccc atcgagctcg gcaagcgcgg
     1921 cctggtggcc acgacgcagg gcctccagac cgtgggtcac aacctctcca acgcgtcggt
     1981 ggacggctac agccgccagc gcgtggagct caccgcctct cctcccctgt acgagccgca
     2041 actgaaccgg gaggagaccc cgggccagat cggccaggga gtcgaggtgg aacgcatcac
     2101 ccgtatccgc gacctgctgc tggaaggccg catcgtctcc gagcagaacg tgcagggcta
     2161 ctgggaggcg cgggacaaat acatcctgat gatggagcag gtatacaacg agcccaccga
     2221 ccactccgtt cgcgcgctct tggacaagtt ctgggagtcg tggcaggacc tctcgctgca
     2281 cccgtcggaa atcg
//
LOCUS       DASRZS010000398         3815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2530999, whole genome shotgun sequence.
ACCESSION   DASRZS010000398 DASRZS010000000
VERSION     DASRZS010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2530999"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFH83_09680"
     CDS             complement(<1..228)
                     /locus_tag="VFH83_09680"
                     /inference="COORDINATES: protein motif:HMM:NF019469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1646 family protein"
                     /protein_id="HET6486678.1"
                     /translation="MGTVGVLAIGVLVLLLPLLVKPVGEQLEVFLFVMGAAAVTVTWQ
                     WSPPLLLQALEEPWKITLAVLAAGLLFHYLRG"
     gene            356..928
                     /locus_tag="VFH83_09685"
     CDS             356..928
                     /locus_tag="VFH83_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6486679.1"
                     /translation="MGDIHTGSRDLHYKPVTRQTIVDLLRLMEKHRTFASCACMRCRL
                     RPARFEALSSRRRREAMQGLVERGSIVGILAYRIDTPVAWCSVAPRESFAALPASHSR
                     APAEDTAVWTVTCFWVTPALRRRGIASALLRAAVEYARTHGARMVEGYPVPAGHPDQR
                     MGSPDIFRRAGFRDVTSEGRRRRVMRNVVG"
     gene            1109..2668
                     /locus_tag="VFH83_09690"
     CDS             1109..2668
                     /locus_tag="VFH83_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006367415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET6486680.1"
                     /translation="MRRFSLRRLIVPGLVMFLVFVVNGIASAQTNGPTIGDVVTATNI
                     MWTFITGFLVFFMQAGFALVETGFTRSKNVAHTMMMNMMVFCIGAIGYWLVGFAFQFG
                     GVGYTFPAIGSIPANSFAPSTLGDFGTHLGRFLIRFGQAGILGGDGFLLTKGVRDVSG
                     ILAFFLFQMVFMDTAATIPTGAMAERLKFSGFVLMGLWVSMVIYPLVGGWVWGGGWLA
                     NLGRTAGLGNGAVDFAGSGVVHMIGGAVAFAGAIAIGPRIGRFNKDGTANAMPGHNIP
                     LGVLGTIILFFGWFGFNPGSSLAFVGGGQNLASNAAVNTLLAGGAGGVSAMLYMWWFG
                     PTKKPDPGLSVNGVLAGLVAITSPCAFVNSTGAVLIGVGAGVIVCLAAFLLEKIHVDD
                     PVGAVPVHFFNGIWGLLSVGIFSDAGASSAGWNGVAGPVTGLIFGGTTQILAQLLEIL
                     SILVVVGGLSFLFFKLLSALKLLRSNPQHELMGLDMPEMGAQGYSNIDVHMPRARLTR
                     QIPGAGPSGGA"
     gene            2685..3071
                     /locus_tag="VFH83_09695"
     CDS             2685..3071
                     /locus_tag="VFH83_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET6486681.1"
                     /translation="MSKKIEAIIREEALDPVKDALREIGIIGLNVFEIRGHGRQGGIP
                     LSWRGTSYQMDLIPKVQVNIILSDNNVEKAIEAIVRAARTGTEGDGIIFIYPVEDIVR
                     IRTGERGHDAIMYPGDIDERRGTKKK"
     gene            3172..>3815
                     /locus_tag="VFH83_09700"
     CDS             3172..>3815
                     /locus_tag="VFH83_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486682.1"
                     /translation="MRRISLNGAWTADLGQGPVTALVPGCWEQLTESKVLTGPVDYKR
                     SFVCPKVSPGGRWLLVFDAVSYYCEASINGRPVGSHEGLWDRFSFDITDALLPEGPNE
                     LTLSIEKPGYAPADRFPLREVLSGFIPDVLCTFGGIWGDVGLIETPGLLAENCTVQTD
                     LTRGMATIAVEAPGSFRDGTIVIRLLRAGAALFEGSLASPGASTASLEIPLRGLR"
BASE COUNT          624 a         1225 c         1293 g          673 t
ORIGIN      
        1 gccgcggaga tagtggaaca ggagccccgc ggccaggacc gcgagcgtga tcttccacgg
       61 ctcctccagc gcctgcagga gcagcggagg gctccactgc caggtgaccg tcacggccgc
      121 cgcgcccatc acgaacagga agacctcgag ctgctctccc accggtttca ccaggagagg
      181 aaggaggagg acgagaacgc cgattgccag tacgccgacc gtgcccatga gtaccctgcc
      241 tcaccgttgt cccgtgccgg taattccggc gacccgacga tgcaccggga gcgaagcccg
      301 tgtctacgcg gccgtcgtcc ggatgccttg gcccgcttat tgcaaaaacc gggccatggg
      361 agacatccac acggggagcc gggacctcca ctacaagcca gtgacgcggc agacgatcgt
      421 ggatctcctc aggctgatgg aaaaacacag gacgttcgcg tcttgcgcct gcatgcgatg
      481 ccgcctgcgc ccggctcggt tcgaagccct ctcctcgcgc cgccgccgcg aggccatgca
      541 ggggctcgtt gagcgcggga gcatcgtcgg catcctggcg taccgcatcg acactcccgt
      601 ggcgtggtgt tcggtcgccc cacgggagtc cttcgcggcg ctgccggcgt cgcatagccg
      661 ggcgccggcg gaggacacgg cggtctggac cgtgacctgc ttctgggtga cgcccgccct
      721 tcgacgccgg ggcattgcct ccgcgcttct ccgtgcggcg gtcgagtacg cgcgcacgca
      781 cggcgccagg atggtcgagg gctacccggt gcctgccggc cacccggatc agcgcatggg
      841 gtcaccggat attttccgca gggctgggtt ccgcgacgtc acctccgagg gccgtcggcg
      901 aagggtgatg agaaacgttg taggatgaat ggtacagaaa tgtaggaaac tgaacgtgcg
      961 gctacatgga tgtatgagtt ccgctcaggg tgccgccatg gttatccgcg attctcaagg
     1021 aaaaaggggc cggcgggcga tctcgttgtc tggcacggat cttgctaaga cccctccatc
     1081 ccctcgcggg gaagaaacag gaggttacat gcgacgattc agtttgcggc gactcatcgt
     1141 accagggctc gtgatgttcc tggtcttcgt cgtcaacggc atcgcctcgg cacagacgaa
     1201 cggtcctacg atcggagacg tcgtaacggc caccaacatc atgtggacgt tcatcacggg
     1261 cttcctcgtc ttcttcatgc aggcgggctt tgccctcgtg gagacggggt tcacgcgctc
     1321 caagaacgtg gcgcacacga tgatgatgaa catgatggtg ttctgcatcg gcgcgatcgg
     1381 ctactggctg gtggggttcg ccttccagtt cggcggcgtc gggtacacgt tccccgccat
     1441 cggctccatc cccgccaaca gtttcgcgcc gagcaccctg ggtgacttcg gcacgcacct
     1501 cggccgcttc ctcatccgct tcgggcaggc aggcatcctg ggaggtgacg ggttcctcct
     1561 gaccaagggc gtgcgggacg tcagtggaat cctcgccttc ttcctgttcc agatggtgtt
     1621 catggacacc gcggccacca tcccaacggg ggccatggcg gaacggctga agttcagcgg
     1681 cttcgtgctc atgggtctgt gggtcagcat ggtgatctac ccgctggtcg gcggttgggt
     1741 ctggggcgga ggctggcttg ccaacctggg acggaccgcg gggctgggca acggggcggt
     1801 agacttcgcc ggttcgggcg tggtgcacat gatcggcggc gccgtcgcct tcgcgggcgc
     1861 gatcgccatc gggccccgta tcgggcgctt caacaaggac ggcacggcca acgccatgcc
     1921 gggccacaac attcccctgg gcgtcctcgg caccatcatc ctgttcttcg gatggttcgg
     1981 gttcaacccc ggctcctcgc tcgcgttcgt gggcggcggc cagaacctgg catccaacgc
     2041 ggcggtgaac accctgttgg cgggcggcgc gggaggcgtc tccgccatgc tctacatgtg
     2101 gtggttcggt ccgacgaaga agccggaccc gggcctctcg gtgaacggcg tccttgcggg
     2161 cctcgtggcc atcacctcac cgtgcgcctt cgtcaacagc acgggcgccg tcctgatcgg
     2221 cgtcggggct ggggtgatcg tctgcctggc cgcgttcctg ctggagaaga tccacgtgga
     2281 cgaccccgtg ggcgcggttc cggtgcactt cttcaacgga atctggggcc tcctgtccgt
     2341 cgggatcttc tccgacgccg gcgcgagctc cgcgggctgg aacggtgttg cgggacccgt
     2401 aacgggtctg atcttcggag gcacgacaca gatccttgcc cagctcctgg agatcctctc
     2461 catcctcgtg gtggtgggcg gactctcctt cctgttcttc aagctcctgt cggcgttgaa
     2521 gctcctgcgc agcaacccgc agcacgagct catggggctg gacatgcccg agatgggggc
     2581 ccagggttac agcaacatcg acgtgcacat gccgcgggcg cggctgaccc ggcagatccc
     2641 tggggccggt cccagcggtg gggcgtgagg gggaggacac ggacatgagc aagaagatcg
     2701 aagcgatcat acgggaggaa gcgctggatc cggtgaaaga cgcccttcgg gagatcggca
     2761 tcatcgggct caacgtcttc gagatccgcg gccacggacg gcaggggggc atcccgttgt
     2821 cctggcgcgg gacctcttac cagatggacc tcatcccgaa ggttcaggtc aacatcatcc
     2881 tgagcgacaa caacgtggag aaggccatcg aggcaatcgt gagggcggcg cggaccggga
     2941 cggagggtga tgggatcatc ttcatctacc cggtggaaga catcgtacgg atccgaaccg
     3001 gggagcgcgg acacgacgcc atcatgtacc ccggcgacat cgacgagcgg cgcggcacca
     3061 agaagaagta gccacgggcg gggagggcgc ctcggcattg ctgccgcgcg ccctcagccc
     3121 cgggctttac ctgccgcccg agccggacct atcctggcct caggagcgcg catgagacgg
     3181 atctctctga acggggcctg gaccgccgac ctggggcagg gaccggtcac ggcactggtg
     3241 cctggctgct gggagcagct cacggaatcc aaggtcctga cgggaccggt ggactacaaa
     3301 cggtcctttg tctgtcccaa ggtgagcccg gggggccgct ggcttctggt gttcgacgcc
     3361 gtgagctact actgcgaggc gtcgatcaac ggacgacccg taggtagcca cgaggggctc
     3421 tgggaccggt tcagtttcga catcacggat gcgctcctgc cggaggggcc caacgagctg
     3481 accctcagca tcgagaaacc gggctacgcg ccggccgacc gcttccccct gcgggaggtg
     3541 ctctcgggct tcatccccga cgtgctctgc acgttcgggg ggatctgggg agacgtcggg
     3601 ctgatcgaga cgccgggact tctggcagag aactgcaccg tgcagaccga tctcacccgg
     3661 gggatggcta ccatcgcggt tgaggcgccc ggctccttcc gggatggcac catcgtcatt
     3721 cgcctccttc gagctggcgc tgcgctcttc gagggctctc tggcgtctcc tggcgcctcc
     3781 acggcgagcc tggagatccc gcttcgagga ctgcg
//
LOCUS       DASRZS010000399         8033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2531410, whole genome shotgun sequence.
ACCESSION   DASRZS010000399 DASRZS010000000
VERSION     DASRZS010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2531410"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..806
                     /locus_tag="VFH83_09705"
     CDS             363..806
                     /locus_tag="VFH83_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486683.1"
                     /translation="MSTRSVQPTLAAIRASLPGEELQRSTLPPLIEMRVEEQNHRVGF
                     RNVHRIPAGSARTVGGGFSSFLVFLVPMPGSIAEIRNENGTFVFRPLRAELFPDVPGP
                     VENCLDRRIPFMSPKGMPLTLVFRPWVSPLDEVNAILRQARPREG"
     gene            complement(850..2199)
                     /locus_tag="VFH83_09710"
     CDS             complement(850..2199)
                     /locus_tag="VFH83_09710"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6486684.1"
                     /translation="MKPTAALVALLVLLSASVAHAQSSGSPDAAAQDLIRQTLLYGID
                     SQVLDAIQKLRASGDKTFTRELASLLTDQRAPAVQTAVLELLRDQDLRDGEDAAKAIL
                     SSPDGVPSDLLSAAIHYASAIKMEGQLALLTPLVDSTDAAASSAAIRALGTSGDPGAV
                     TLLLAKLKDPGFPDGRRNDVILALGDLKSSQATEQLAAIAKNADEDQVRRLYAADSLG
                     KIGDAQALPVLKGMFAEPSALVRAYAASALANFSLDDVLPMLVSGLKDENDKVRLECA
                     KALARTMSSSQAASAVPVLSYKARFDPVGTVRVQSIQTLGSIGTDACFPVLRDLYTAA
                     GSSMDGREAALTALVAKWLPQSLPSIRDVVGQGVLQVDQRILEITGRILATAKDPGLR
                     EVYASLLGSKSAAARYSAIQGIAANRFSDLKDRVKKVADTDPAGAVRQQAQAALSQL"
     gene            complement(2196..3761)
                     /gene="murJ"
                     /locus_tag="VFH83_09715"
     CDS             complement(2196..3761)
                     /gene="murJ"
                     /locus_tag="VFH83_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET6486685.1"
                     /translation="MGEEESRAGRTSFSAVVVMACTAASRLFGYVRSALIALYFGASG
                     DADALNAVYMIPNNLRKLFAEGAFASAFIPSLATAINEDRSLERSRELVRSLIGFLLV
                     VLVPFVALSLAFPRAFVAVLLQFPDPHKADTAAQLMRWIFNYILLVSLSGLVQAALNT
                     HGSFAVPALSPLLFTLATVLCLVFLHQRYSVLSQGVGVLAGGVLQLAFQVPALRRKGY
                     RFLPSFRFRNPDFVRTIKLWVPYLVSASIVAVNQQIAQLFASWLENGSISAVNNSVLF
                     FQIPIGIFTASIVTVLFPRMSRQVADGDTAALRGSVSYGIQSILVLLIPSSVLLALFG
                     REIISALLQRGAFSSDNTLMTSRALTGYAVGLASMGLYTFLQRFFYSLKSFAVPLYSA
                     GFVAAVDVAASLILIRTPLRVSGLAYANSISFTAGFILLLVLAFRRLGSLEALPILRS
                     LGKALVGSIPMAAGLALFLRWKPDLWLHGGGLRSAALICVVAAAAVAMTLGAYVALRV
                     PFVTELYRRRVRR"
     gene            complement(3764..4552)
                     /locus_tag="VFH83_09720"
     CDS             complement(3764..4552)
                     /locus_tag="VFH83_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HET6486686.1"
                     /translation="MKRRPWIVVALVLSGYAVFAQPARARTEDSPGGSLPGSLVRFSA
                     RLLGTPYVVGGDSVQGVDCSGLISLVYRRTVGIALPGSVGALFGLGTRASFPLHLGDL
                     LFFDTASVSVPSHPTHVGIYIGQGRVVHAASEGPRTGVIVSSLSEAYYQQRFLAARRV
                     FPWPRPTLDMILTDDHRCVLAGLSYPSHVPLQIAVYNRMTGGGPMDLTVSRDDQPVMA
                     QRVAPTAGKPVLLDLMPDIGRWTVAVTRILKGRTLGSLTFQVDE"
     gene            complement(4588..5715)
                     /gene="tgt"
                     /locus_tag="VFH83_09725"
     CDS             complement(4588..5715)
                     /gene="tgt"
                     /locus_tag="VFH83_09725"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET6486687.1"
                     /translation="MSTFSLSTRGATGAGRAGALALGHGTVMTPCFMPVGTNASVKAM
                     DHAGLESMGINLILGNTYHLYLRPGKEVIRAAGGLHSFMGWRHNILTDSGGFQIFSLS
                     SFRTIDDGGVLFRSHIDGSSHRLTPEGVIDIQSVLGSDVMMPLDECTSPFIPKPAAEA
                     AMRRTTSWLEASRRRWAERTGPLVGQLFGIMQGNFYEDLRRESAYRMAELDLPGYAIG
                     GLSVGEEFSVFRDFLHLSASLLPADKPRYLMGVGTPEYILEAVDAGVDLFDCVFPTRT
                     ARNAQVFTRDGTLSLRNERFKLDFQPIDPECRCPTCGGHSRAYLRHLFKAHEIQAAVL
                     ATRHNLSFIHTLVHDIRMAVELGRFPAFKRDFLARYRDAAR"
     gene            complement(5712..6665)
                     /locus_tag="VFH83_09730"
     CDS             complement(5712..6665)
                     /locus_tag="VFH83_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6486688.1"
                     /translation="MEYAAGVDFGGTSAKIGLVDRSGVVLQRAEVVMDPRETYEGLLR
                     PVADRLRGMVGRQEGRLLTVGIGTPGFIDKRRGLIIGGCVNLPALQGRSVQEYLGAEL
                     GVPAFSENDATSAAAGELAFGAGRRFSHFALITIGTAIGGGLVLDGKVYRGSRGLAGE
                     LGHLCMVPEGLWCNCGSRGCFEQYASATAIVRLYDEKARKRQDPPEAQTSPQPAIGTR
                     EIVERARGGEAIARDTLEEAGQWIAQAFGTLMNALDLQACLVGGGFSQAGEAILEPIR
                     RHLPDHCWPQVGRGVQVLAAELANDAGILGAAAQAMERLGA"
     gene            complement(6674..7753)
                     /locus_tag="VFH83_09735"
     CDS             complement(6674..7753)
                     /locus_tag="VFH83_09735"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HET6486689.1"
                     /translation="MSLLRRLLLRQFVPIFLVAILFFVLLLQLMDIFGSIWRYVAHDV
                     PVREVAKIALLYVPKCVSYALPVSFLFAIAYSLGILYANNELFAIFCSGTSLHRLVLP
                     FLFLGALFSVGSFYFEDSVVIPTFRTKNDLYAVAVKQMESLSQSNVTVTSPDQRVIYQ
                     ADYYNDAQTRLNGLTVVIRDEKMSPTSRIDASWAEWSKTRWVLHDCRIFTVDPATGLV
                     TDVRMPSYDSPQIVEPPDTFRKLARNIEEMSAGEAGRYVALIRKAGLFYWDALTDYYR
                     KYSFACTPFVVAFIASSLGSTFKKNILLMSLLSSLVISVVFYVAQMVAAILSKNQIIP
                     PLAGAWSPFSLFLLLGFVLFRTART"
     gene            complement(7750..>8033)
                     /locus_tag="VFH83_09740"
     CDS             complement(7750..>8033)
                     /locus_tag="VFH83_09740"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HET6486690.1"
                     /translation="QSYEVEFYRKFSMPAACLVFSFFAFPVGQRARRSGRTVGFAVGL
                     FVAIAYWGMLVAGQTFGVRMSLSPAFSMWFPDVLVLAIGGVLLLGRLRR"
BASE COUNT         1496 a         2617 c         2714 g         1206 t
ORIGIN      
        1 ccccggcggc cgtctctgcg cctcggccgg ggggtaggaa gcaggcggcg cttcaggcgc
       61 agccgacgcc cgcccttgcg cgggtggctg agatccccga accgagggag acgcgggaat
      121 cgaagaaagc gccgactccc gtgcccggtg gcctgggaag gcagccctgg tggcgccgtg
      181 gacgcggaga gccgaagccc ggtcttccag gagcgccagt tggtgcggct cgcaaggccg
      241 agcccgcggc tcccgcgctc tcgcagaagg ctgtctctcg aggcgcaccg gctcccatgg
      301 tccagagact ggcggcccca acgcccgcga tgccctccag gcggggccct ccggcacgaa
      361 cggtgtcgac tcgcagcgtc cagccgaccc tggcggcgat ccgcgcctcg ctccccggcg
      421 aggagctcca gagatccaca ctcccgccgc tcatcgagat gcgcgtggaa gagcagaacc
      481 atcgcgttgg attccgaaac gtccatcgga tcccagccgg atccgcccgc acggtaggcg
      541 gaggattctc ttccttcctc gtcttcctgg tgccgatgcc gggctcgatc gccgagattc
      601 gcaatgagaa cggcaccttc gtcttccggc cgctccgagc cgagctgttt ccagacgttc
      661 ccggtcccgt ggaaaactgc ctggaccggc ggatcccgtt catgagcccg aaggggatgc
      721 cgctgacgct ggtgttcagg ccctgggttt ctccgctcga tgaggtaaat gcgattctga
      781 ggcaggccag gccacgggag ggctgaccgg gcgaacggcg taggggcggg cccgacgcca
      841 tcccggctgc tacagctggg acaacgctgc ctgggcttgc tgtcggaccg ctccggcagg
      901 gtccgtatcg gcgaccttct ttacacggtc cttgaggtcc gagaaccggt tcgcggcgat
      961 tccctggatg gcgctgtacc tggccgccgc gctcttgctt cccagcaggg aggcgtagac
     1021 ctcccggagg cccggatcct tggcagtggc gaggatgcgg ccggtgatct ccaggatccg
     1081 ctggtctacc tggaggactc cctgccctac cacgtcgcgg atcgatggga gagactgcgg
     1141 cagccacttc gcgacgagcg cagtcagcgc tgcttcccgt ccgtccatcg agctacccgc
     1201 cgccgtgtac agatccctca ggacggggaa gcaggcatcc gtgccgatgg accccagcgt
     1261 ctggatggac tggacccgga ccgtgcccac cgggtcgaac ctcgccttgt agctcagcac
     1321 cggcaccgcg gatgccgcct ggctcgaaga catggtccgg gccagcgcct tggcgcactc
     1381 gagacggacc ttgtcattct cgtccttcag cccggagacg agcatgggaa ggacgtcgtc
     1441 cagcgagaag ttggccagcg cggaagccgc gtatgcgcgg acaagggcgc tcggctcggc
     1501 gaacatcccc ttgagaacag gcagcgcctg cgcatctccg atcttgccca gggaatccgc
     1561 ggcgtagagc cgccggacct ggtcctcgtc ggcgttcttc gcgatcgcgg cgagctgttc
     1621 cgtcgcctgg gagctcttca gatcccccag ggcaaggatg acgtcgttcc gtctgccgtc
     1681 cggaaagccc gggtccttga gctttgccag gagaagggtc acggcgccgg gatccccgct
     1741 cgtgcccagg gcccggattg ccgccgatga agcggcagcg tccgtcgaat ccaccagcgg
     1801 cgtcagcagc gcgagctggc cttccatctt gatcgcggag gcatagtgga tggccgcgct
     1861 gagcaggtcg ctgggcaccc cgtccggcga gctcaggatg gccttggccg cgtcctcacc
     1921 gtcgcgcaag tcctggtccc gaagcagctc caggacggcc gtctgtaccg ccggcgcccg
     1981 ctggtccgtg agcagcgagg ccagctccct ggtgaaggtt ttgtctccgg atgcgcgaag
     2041 cttctgaatg gcgtccagaa cctgactgtc gatcccgtag agcagggtct gtcgaatcag
     2101 gtcctgtgcg gccgcatccg gggacccgct gctctgagcg tgggcaaccg aagccgacag
     2161 caggacgagc agcgccacga gggccgccgt gggcttcatc gcctcaccct cctgcgatac
     2221 agctcggtca cgaacggcac ccggagcgcg acgtacgcgc ccagcgtcat ggcgacggcg
     2281 gcggctgcca cgacgcagat gagcgcagcg gagcgaagac cgccgccatg gagccagaga
     2341 tcgggcttcc agcgaaggaa cagcgccaga ccggcagcca tggggatgga cccgaccagg
     2401 gccttcccca gggaccggag gatgggcagg gcctccagcg agccaagtcg gcgaaacgcg
     2461 aggaccagga gaaggatgaa cccggcggtg aacgagatgg agttggcgta ggccagtccg
     2521 ctgactcgca gcggagtccg aatcaggatc agagaggcgg ccacgtccac cgcggccacg
     2581 aagccggcgc tgtagagagg gaccgcgaag ctcttgaggg agtagaagaa cctctggagg
     2641 aacgtataga gccccatgct ggccaggcct accgcgtagc cggtcagcgc ccgcgaagtc
     2701 atcagggtgt tgtccgagga gaacgccccg cgctgaagca gggcggagat gatctcgcgg
     2761 ccgaagaggg ccaggaggac ggaagacggg atcaggagga ccaggatgga ctggatgccg
     2821 taggagaccg agcccctcag cgccgccgtg tcgccgtccg ctacctgccg actcatccgg
     2881 ggaaaaagga ccgtgacgat cgatgcggtg aagatcccga tggggatctg gaagaagagc
     2941 acggagttgt ttacggcgga gatgctcccg ttttccagcc agctcgcgaa gagctgggcg
     3001 atctgctggt tgaccgccac gatggaggcc gacaccaggt acggcaccca aagcttgatg
     3061 gtccgcacga aatcggggtt gcggaaccgg aacgacggga ggaaccggta ccctttccgc
     3121 cgaagggccg gcacctggaa cgcgagctga aggacgccgc cggccaggac gcctacgccc
     3181 tgggagagca cgctgtagcg ctggtgcagg aacacgaggc agaggaccgt ggccagcgtg
     3241 aacaggaggg ggctcagcgc cggcacggca aaggagccgt gcgtgttcaa ggccgcctgg
     3301 accagcccgc tcagcgagac caggagaatg tagttgaaga tccatcgcat gagctgcgcc
     3361 gcggtatccg ccttgtgcgg atccgggaac tggagcagga cggcgacgaa ggccctcggg
     3421 aacgccaggc tcagcgcgac aaacggaacc agcacaacca gaaggaagcc gatgagggag
     3481 cgaaccagct cccgagagcg ctccaggctc cgatcctcgt tgatggcggt ggcgaggctc
     3541 gggatgaacg cggaggcgaa ggcaccctcc gcaaacagct tgcggaggtt gttcgggatc
     3601 atgtagacgg cgttgagggc atccgcgtct cccgaggcgc cgaagtacaa cgcgatgagg
     3661 gcgctgcgaa cgtaaccgaa aaggcgggaa gccgcggtac aggccatcac caccacggcg
     3721 gagaaggagg tcctgccggc tcggctctct tcttctccca tgactactcg tccacctgga
     3781 aggtcaggga ccccagggtc ctgcccttca ggattcgcgt cacggcgacg gtccagcgcc
     3841 cgatatcggg catcaggtcg agcaggaccg gcttcccggc cgtgggggcg accctctgtg
     3901 ccatcacggg ctggtcgtcc cggctcacgg tcaggtccat gggacccccg ccggtcattc
     3961 ggttgtacac cgcgatctgc aggggcacgt gggaaggata ggacagacct gccagaacgc
     4021 agcggtggtc gtccgtcagg atcatgtcca gcgtcggcct gggccaggga aacacccgac
     4081 gcgccgcgag gaaccgctgc tggtagtacg cctccgagag ggaagacacg atgacgccgg
     4141 tcctcggtcc ctcggacgcc gcgtgcacca cgcggccctg gccaatatag atgccgacgt
     4201 gcgtagggtg ggacgggacc gaaacgctgg ccgtatcgaa aaagaggagg tcgccgaggt
     4261 gcagtgggaa gctggcccgc gtcccaagcc cgaagagagc tccgacgctt ccgggcagcg
     4321 cgatgccgac cgtcctgcgg tagaccagcg agatgaggcc cgagcagtcc accccctgga
     4381 cactgtcacc cccgaccacg tagggagtcc ccaggagcct ggcgctgaac cgcaccagag
     4441 aacccggcag agatccgccc ggggagtcct ccgtccgggc ccgggccggc tgcgcgaaga
     4501 cagcgtatcc cgagaggacg agagcaacga cgatccatgg gcgtctcttc atgcggctgg
     4561 ccttcatcct gatggtcggc acttccctca ccgcgctgca tcgcgatacc gggccaggaa
     4621 atccctcttg aaggcaggga accgacccag ctcgacggcc atccgtatgt cgtggaccag
     4681 cgtgtggatg aagctcagat tgtgccgggt ggccagtacc gccgcctgga tctcatgcgc
     4741 cttgaacagg tgccgcaggt aggccctgct gtggccgccg caggtcgggc atcggcattc
     4801 gggatcgata ggctggaaat ccagcttgaa gcgctcgttt cggagggaaa gggtgccgtc
     4861 ccgggtgaag acctgggcgt tacgagcggt gcgggtagga aagacgcagt cgaagaggtc
     4921 caccccggcg tccaccgcct ccaggatgta ttccggagtg ccgacgccca tcaggtagcg
     4981 aggcttgtcg gccggcagga gcgatgcgga gaggtgcaga aagtcgcgaa acacggagaa
     5041 ctcctctccc acggacagac caccgatggc ataacccggt agatccagct ccgccatccg
     5101 gtaggcgctt tcccggcgga ggtcctcgta gaagttgcct tgcatgatcc cgaacagctg
     5161 accgaccagg ggtccggttc gttccgccca ccgcctgcgg ctcgcttcca gccaggacgt
     5221 ggtcctgcgc atcgcggcct ctgcggccgg cttgggaatg aagggcgacg tgcattcatc
     5281 cagcggcatc atcacgtcgc ttcccaggac cgactgaatg tcgatgacgc cctccggggt
     5341 caggcgatgg gacgacccat cgatgtgcga gcggaagagg actccgccgt cgtcaatcgt
     5401 tcgaaacgac gaaagggaga agatctggaa gcctccggag tccgtgagga tgttgtgccg
     5461 ccagcccatg aaggagtgta gcccaccggc tgcccggatc acctctttcc ccggcctgag
     5521 gtacaggtgg tacgtgttcc ccagaatgag gttgatcccc atgctctcga gaccggcgtg
     5581 gtccatggcc ttgacgctgg cgttggttcc aaccggcatg aagcaggggg tcatgacggt
     5641 gccatggccc agggccaggg cacccgcccg accagcgccc gttgcgcccc gggtggacag
     5701 ggaaaacgta ctcacgcccc gagtcgctcc atggcctgcg cggcggcgcc gaggatccct
     5761 gcatcgtttg cgagctccgc cgccaggacc tggacgcccc gacccacctg gggccagcag
     5821 tggtccggaa ggtgccgtcg aatgggctcc aggatggcct cccccgcctg ggaaaatccg
     5881 cccccaacga ggcaggcctg caggtccagg gcgttcatca gcgtgccgaa cgcctgggcg
     5941 atccactgtc ccgcctcttc cagggtgtcc cgcgcaatcg cttccccgcc acgggcgcgc
     6001 tccacgatct cgcgggtccc gatggcgggc tgcggtgacg tctgcgcctc gggagggtcc
     6061 tgccgctttc gagccttctc gtcgtacaga cgcacgatcg ccgttgcgga cgcatactgc
     6121 tcgaagcagc cgcggctccc gcagttgcac cacaggcctt cgggaaccat gcagaggtgc
     6181 cccagctccc cggccagtcc gcgggagccg cggtacacct tcccatccag caccagcccg
     6241 ccgccgatgg cggtcccgat cgtgatgagc gcgaaatggg agaaccgcct gcccgccccg
     6301 aaggccagct ctcccgccgc cgcggaggta gcgtcgttct ccgagaaggc cggcactccc
     6361 agctcagcgc ccaggtactc ctgcacggaa cggccctgga gggcgggcag gttgacgcac
     6421 cctccgatga tgagaccccg ccgcttgtcg atgaaaccgg gcgtgccgat gcccaccgtg
     6481 agcagccgcc cttcctgccg accgaccatg cctcgcagcc ggtccgctac cggccgcagg
     6541 agaccctcgt aggtttcccg agggtccatg acgacttcgg cccgctggag cacgacgccg
     6601 gaccggtcga ccagaccgat cttggcagag gtccccccga agtccactcc cgcggcgtat
     6661 tccatgtcac cccctatgtg cgtgcggttc ggaatagcac gaaacccagc agcaggaaaa
     6721 gggagaacgg gctccaggct ccggccaggg gagggatgat ctggttcttg gagaggatgg
     6781 ccgccaccat ctgggcgaca tagaagacca ccgagatcac cagagaggag agcaggctca
     6841 tcaggaggat gtttttcttg aacgtgctgc ccaggctgct ggcgataaag gcgaccacaa
     6901 agggcgtaca ggcgaaggag tacttgcggt agtagtccgt gagggcgtcc cagtagaaga
     6961 gcccggcctt gcggatgagg gccacgtacc tgccggcttc ccccgcgctc atctcctcga
     7021 tgttccgcgc cagcttgcgg aaggtgtccg gcggctcaac gatctgcggg gagtcgtagc
     7081 tgggcatgcg gacgtcggtc accagaccgg tggccggatc taccgtgaag atccggcagt
     7141 catgcaggac ccatcgcgtt ttgctccatt ccgcccagga ggcgtcgatc ctgctggtcg
     7201 ggctcatctt ctcgtcgcga atgacgaccg tcagcccgtt gagacgcgtc tgcgcgtcgt
     7261 tgtagtaatc ggcctggtag atgacgcgct ggtccgggct ggtcaccgtc acgttggatt
     7321 ggctcaggga ttccatctgc ttcacggcca cggcgtacag gtcgttcttg gtcctgaacg
     7381 tggggatcac cacgctgtcc tcgaagtaga aggacccgac gctgaacagc gcccccagga
     7441 acaggaaggg aagcacgagg cggtggaggc tggtgccgga gcagaagatc gcgaacagct
     7501 cgttgttggc gtagaggatc cccaggctgt aggcgatggc gaacagaaac gatacgggaa
     7561 gcgcatagga aacgcacttc ggcacgtaga gcagcgcaat cttggcgacc tcccgaaccg
     7621 ggacgtcgtg ggcgacgtat cgccagatgc tcccgaaaat gtccatgagc tgcagcagga
     7681 gcacgaagaa gaggatggcc accaggaaga tgggaacaaa ctgcctcagg agaagccgcc
     7741 tcaggaggct caccgccgga gcctccccag caggagcacc cctccgatcg cgagcaccag
     7801 cacgtccggg aaccacatgg aaaacgccgg tgagagcgac atccgcacgc cgaacgtctg
     7861 gcccgccacc agcatgcccc agtacgcgat agccacgaag aggcccacgg cgaatcccac
     7921 ggtccggccg ctgcgccggg cccgttggcc cacggggaag gcgaagaacg agaacacgag
     7981 gcaggcggcc ggcatggaga actttcggta gaactccacc tcgtaggact gca
//
LOCUS       DASRZS010000400         4103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_609854, whole genome shotgun sequence.
ACCESSION   DASRZS010000400 DASRZS010000000
VERSION     DASRZS010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609854"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..1839
                     /locus_tag="VFH83_09745"
     CDS             16..1839
                     /locus_tag="VFH83_09745"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6486691.1"
                     /translation="MPNRPGTRHVHCAACLVGAAILLCSGCAVTQPRPSDIAAGGRAT
                     AITKVAEAAKGFMRSHRVQGLSVAVADSEGLIWAGGFGTADRRNGRAFTEDTRSLPGS
                     VSKLFTTTAVMILADRGQVDLDAPLVRYLPDLAVRTDGWPVGTITVRSLLTHHSGLPS
                     DRFKGMVSDLPPTPGAETPDEVPALLRDDFAPRPPDTAFAYSNLGFSLLGALVERVSK
                     EPFDRFVREEVFQPLGMTSSAFAFGEADRQAASRGYRGTSEQLVPYLRDIGAGGLLTT
                     ARDMGRFLSGMLAVNRGKPGILRTDTLAAMWTRQNTTIAEDLDFKIGLGWWLGPFDSL
                     PGERVIAHGGDWLPFCSLAMVFPDRNLGIFIMANSLDGSGSMTLEDLALPTVRAFAAS
                     ERHAELPSTPIVTTSRVIPPSLRTAVTGDWTSMVGLLRVQEANGGLRVFFAGKWFDCV
                     LRSDGRLGIEARILGIKLPVAELEEYTISADAVNGEPVLGFRCHGILLSVCHRVYDAP
                     VDPAWMARVGPWRIPAADAQGLVQGVQLTTDDAGRLLLSYRFFGNPTPRVYPLETLSP
                     TRARLMGTGRSLGESIVAEGDGTDERLLWSGFSLSREPPAR"
     gene            complement(1885..2394)
                     /locus_tag="VFH83_09750"
     CDS             complement(1885..2394)
                     /locus_tag="VFH83_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6486692.1"
                     /translation="MQETAKQYTERILGYLDGKKPMEVLAATPRQIGRLVKGVPRKRL
                     SQRPAVDTWSVTEILAHLADSEMVYGYRIRMILEANGIPIQATDQDAWARFSEYGKQD
                     PALSLQAIRVGRERTIRLLKGLPKESWERYGTHSERGKETVARVVEMLAGHDLNHLRQ
                     IRERLPRKR"
     gene            complement(2496..2810)
                     /gene="rhaM"
                     /locus_tag="VFH83_09755"
     CDS             complement(2496..2810)
                     /gene="rhaM"
                     /locus_tag="VFH83_09755"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HET6486693.1"
                     /translation="MTRAAFTMKLKPGAQAEYKRRHDEIWPDLVKELEAAGVSDYSIY
                     LDEATLTLFAFQKLKDHNSSELLPSREVVKKWWKYMKDLMDCNPDNSPIMGPLKEVFH
                     MD"
     gene            2902..>4103
                     /locus_tag="VFH83_09760"
     CDS             2902..>4103
                     /locus_tag="VFH83_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HET6486694.1"
                     /translation="MRSRIGLFGIGLDTYWPQFAGLKERLLGYQGYIARRLAAMDVSV
                     EDAGLVDSVDRGREAARLFRERGVDGIFLNISTYALSQTVLPVAQQVRVPIVVLNLQP
                     VPAIDYARFNAIGDRGVMTGEWLAHCQACAAPEIANLFGGCGIDYHPVTGWLEEPYVW
                     EQIGGWVDAIRVRRELQETRVGILGHYYGGMLDVYTDVTRLADVFGSHFELIEMDRLR
                     HLRDQVETAEVTRKLAQFQTEFVVSPECSAAELQRAARTSCALDALTAEARLGALAYY
                     YEGQPGSEHEDIVTSVIPGNTLLTAHHVPVAGECEVKNVIAMKILDSLGAGGSFSELY
                     AIDYPSDVVLWGHDGPAHPAIAEGPVGLVPLPVYHGKPGKGLSIQMSVRNGPVTLLSV
                     VQGRGGRT"
BASE COUNT          631 a         1387 c         1358 g          727 t
ORIGIN      
        1 ccaaggagcc ataccatgcc gaaccgtccg gggacgagac acgtccattg cgcggcgtgt
       61 cttgttggcg ccgctatcct cctctgcagc gggtgcgccg tgacgcagcc aagaccgtct
      121 gacatcgccg ccggaggaag ggcgaccgca atcacgaagg tcgccgaagc cgcaaaggga
      181 ttcatgagga gccaccgggt ccaggggctc tccgttgcgg tggccgactc ggaaggtctg
      241 atctgggccg gcggtttcgg cacggcggac aggcggaacg ggcgcgcctt cacggaggat
      301 acccggtcgc tgcccgggtc ggtctccaag ctgttcacga caaccgccgt gatgatcctc
      361 gccgaccgtg gccaggtgga tctcgacgct cccctggtgc ggtatctgcc ggacctggcc
      421 gttcggaccg atggctggcc cgtcgggacc atcacggtcc gcagcctcct cacccaccac
      481 tcgggattgc cgtcggaccg tttcaaggga atggtctccg acctcccgcc gacaccgggc
      541 gccgaaaccc cggatgaggt gccggcgctg cttcgggacg acttcgcgcc ccgcccgccg
      601 gacacggcat tcgcctactc caacctgggg ttctccctgc ttggcgccct ggtggagcgc
      661 gtcagcaagg agcccttcga tcgattcgtc cgcgaagagg tcttccaacc cctcggtatg
      721 accagctccg cgttcgcctt cggagaggcc gaccgccagg cggcgtcccg tggctaccgg
      781 ggaaccagcg agcagcttgt cccgtacctg cgggacatcg gcgccggcgg tcttctcacc
      841 accgcccgcg acatgggccg cttcctctcc ggcatgctgg ccgtcaaccg gggcaagccc
      901 ggtatcctgc ggaccgacac gctcgcggcc atgtggacgc ggcagaacac gaccatcgcc
      961 gaagacctgg acttcaagat cgggctcggt tggtggctcg gccccttcga cagcctcccg
     1021 ggagagaggg tcattgccca tggcggcgat tggctcccct tctgctccct cgccatggtc
     1081 ttcccggacc gcaacctcgg catcttcatc atggcgaact ccctggacgg cagcggctcc
     1141 atgaccctgg aggacctggc tctacccacg gtgcgcgcct tcgccgcgag cgaacggcac
     1201 gcggagctcc cgagcacgcc gatcgtcacc accagccgcg tcattccacc gtcgcttcgg
     1261 accgcggtta ccggggactg gacctccatg gtggggctgc tgcgcgtcca ggaggccaac
     1321 ggcgggctgc gggtcttttt cgccggtaag tggttcgact gcgttcttcg gagcgacggt
     1381 cggctgggga tagaagcccg gatcctgggg atcaagctgc cggtcgcgga gctggaggaa
     1441 tacaccatca gcgcggacgc cgtgaacggg gaaccggtgc tcggcttccg ctgtcacggc
     1501 atcctgctct cggtatgcca tcgcgtgtac gacgctcccg tcgaccccgc gtggatggcg
     1561 cgcgtcggcc cgtggcgcat tcccgccgcg gacgctcagg gcctggtcca gggcgtgcag
     1621 ctcacgacgg atgacgcggg tcgcctgctt ctcagctacc ggttcttcgg caacccgact
     1681 ccacgggtgt atccgctgga gacgctgtcg ccgactcggg cgcgccttat gggaaccgga
     1741 cgctccctgg gagagagcat cgtcgcggaa ggggacggga ccgacgagcg cctcctctgg
     1801 tccggcttct ccttgtcacg agaaccgccg gcgcgatagc agaaccacgg ccttcccgcc
     1861 cggagctaaa gaaggcgcgg acccttatcg cttcctgggc agccgctccc ggatctgtct
     1921 caggtggttg aggtcgtggc ctgccagcat ctctaccacg cgggccacgg tctctttgcc
     1981 gcgctcggag tgcgtcccgt agcgctccca gctctccttg gggaggcctt tcaggagacg
     2041 gatcgttcgt tcccgaccga cgcggatggc ctggagggag agcgccggat cctgcttccc
     2101 gtattcggag aaccgcgccc aggcatcctg gtccgtcgcc tggatcggga taccgttggc
     2161 ttcgagaatc atgcggatgc gatacccgta caccatctcg ctgtcggcca ggtgcgccag
     2221 gatctcggtg acggaccagg tgtccacagc ggggcgctga gacagccgct ttcgcgggac
     2281 tcccttcacc aggcgcccga tctgcctcgg ggtggcggcc aggacctcca tcggcttctt
     2341 cccgtccagg taacccagga tccgttccgt gtactgtttc gccgtttcct gcatctcggg
     2401 gctccttcct cgggatccaa ggtacgagag gccgtccacc cgaaacttgc ggtgcacacg
     2461 gccggcaccc gacgaggggg ccggcgcgga ccggctcagt ccatgtggaa gacttccttg
     2521 agcggcccca tgatgggcga gttgtccggg ttgcagtcca tgaggtcctt catgtacttc
     2581 caccacttct tcaccacttc gcggctgggc aagagctccg agctgttgtg gtccttgagc
     2641 ttctggaacg cgaagagggt cagcgtggcc tcgtccaggt agatggagta gtccgacacg
     2701 ccggcggcct ccagctcctt gacgaggtcg ggccagatct cgtcgtggcg gcgtttgtat
     2761 tccgcctggg caccgggctt cagcttcatg gtgaatgcgg ctctggtcat tcgtttctcc
     2821 tcgcgggagg ctacacgatc ccgtggatca ccgcaagttt ggttgccgtc atcgccttcc
     2881 gggagcaata ttgcagtaac cgtgcggagc aggatcggac tcttcggcat aggcctggac
     2941 acgtactggc cccagttcgc gggcctgaag gaacgcctcc tcgggtacca gggctacatc
     3001 gcgagacgtc tcgcggcgat ggacgtctcg gtcgaggatg ccggcctggt ggactccgtg
     3061 gaccggggac gcgaagcggc ccggctgttc cgcgagcggg gcgtagacgg gatcttcctg
     3121 aacatctcga cctatgcgct ttcccagacg gtcctcccgg tggcgcagca ggtgcgggtg
     3181 cccatcgtgg tgctgaacct gcagcccgtg cccgccatcg actacgcccg gttcaacgcc
     3241 atcggggacc gcggcgtcat gaccggggag tggctggcgc actgccaggc ctgcgccgca
     3301 ccggagatcg ccaacctctt tggtggctgc gggatcgact atcacccggt gaccggctgg
     3361 ctggaggagc cctacgtctg ggagcagatt ggcggctggg tggacgccat ccgagtccgg
     3421 cgcgagctcc aggaaacgcg cgtcggcatc ctgggccact actacggcgg gatgctggac
     3481 gtgtacacgg acgtgacgcg ccttgcggat gtgttcggca gccacttcga gctcatcgaa
     3541 atggacaggc tccgacacct tcgggaccag gtggagaccg ccgaagtcac gcggaagctc
     3601 gcccagttcc agacggagtt cgtggtgtcc cccgagtgct ccgccgcgga gctccagcgg
     3661 gccgcccgga cctcctgcgc gctggacgcc ctgacggcgg aggcccggct cggcgccctg
     3721 gcgtactact acgagggcca gcccggtagc gagcacgaag acatcgtgac gtcggtcatc
     3781 ccggggaaca cgctcctgac ggcccaccac gttcccgtgg ccggcgagtg cgaagtcaag
     3841 aacgtcatcg caatgaagat cctggattca ctcggagcgg gaggctcctt ctcggagctg
     3901 tacgcaatcg actatccctc agacgtggtg ctctggggcc acgacgggcc ggcccatccg
     3961 gcaatcgcgg agggccccgt aggcctggtc ccgcttcccg tataccacgg gaaaccgggc
     4021 aaggggttgt ccatccagat gagcgttcga aacggcccgg tcaccctgct ctccgtggtg
     4081 cagggtcgag gcggccggac gtt
//
LOCUS       DASRZS010000401         5392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2142183, whole genome shotgun sequence.
ACCESSION   DASRZS010000401 DASRZS010000000
VERSION     DASRZS010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2142183"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VFH83_09765"
     CDS             <1..421
                     /locus_tag="VFH83_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486695.1"
                     /translation="SDDLLHWESRELDSAWPGGECAVAFASYHPTNRDHLVLMTGGNH
                     TGHFYAIGEVLFSLRDPETPIAWLPRPVLAADATIPYEDGCSAEPPHRPVSYWRDTVF
                     VCGMTLFKGSWLAYYGGSEYYTCLATAPARGGGTECS"
     gene            412..1362
                     /locus_tag="VFH83_09770"
     CDS             412..1362
                     /locus_tag="VFH83_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020617177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1861 family protein"
                     /protein_id="HET6486696.1"
                     /translation="MLMNTQRELFEKSKVVYESAALSFRGVEGFDVYNTSVPFVWKGR
                     SFLLGRVERRADWARSWVRLFAPTGPDEWTLVANAMIYPLEDPFIAWIGGELVMGGTH
                     VRYRAGEIDTLYTYFYRGSELHDLRYFTTGPDAMKDIRLVQLADQRIGVFSRPRNQEI
                     VRRFGTEAQIGFTVIDRLEDLTSDVIEQAAYIPGLFAQGEWGGCNQALLLENGRIGII
                     GHKSYTHGAADGAKGMVYVNVSFVFDPAKREASDLKIIGTRTCYPQGPAKRPELGDCA
                     FTSGIVMRPDGRADLYSGIGDTGEGRITIDYPFPARIVSP"
     gene            1530..3206
                     /locus_tag="VFH83_09775"
     CDS             1530..3206
                     /locus_tag="VFH83_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6486697.1"
                     /translation="MRRHGVPRVLVVLAVFAGLAVSAGAQTAVDKLPVTKLPPITISV
                     FTVNTQFPLPPDDNRAYQLIKDKLGVTFKWDIAVGEADQKIGVMIAGQDYDDLLHVNS
                     PKFIDAGALIPLEDLIDKYAPHLKAHYESDPVTWAKMHEKDGHVYTLLDYGVIDNGPT
                     ATDYYGAAMFIQKAVMRESNFPKIATIDEYFDLISRYKARHPTTADGKPTIGFSILTY
                     DWHKFDLVNPPPFLAGYPNDGDGIVDPVTHKYRLQYYGPEAHRWFKLLNQMNDKGLVD
                     HDSFVDNYDQYLAKLANGQVLGVHDQYWQFQTASQTLVSQNRIWDTMMPLPIVFDKSI
                     RPHWRDSPLPNLQRGYGISIEAKDPVRIIKFLDEQYDPAWYKVFQWGLEGVDYSIDND
                     GQPVMTPLQRQHYDDVTWWLHNLAELWWQEAPKYEGHFTRGGLAAPLRDNPKEYFASL
                     HPEDAQILKAYGATSWSDLMDHNTPPNPPWYPAWQITPSDGTPAQLAFAKAQQVYVKD
                     LPKVILAKPADFEKEWQSYVNDLMQANVKVFEDFIQAGIDDRMARYGQKR"
     gene            3321..4247
                     /locus_tag="VFH83_09780"
     CDS             3321..4247
                     /locus_tag="VFH83_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007432973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6486698.1"
                     /translation="MSSIVPGKQGFWSRLAAQRQLAWMSVPLVLYVLLFTYVPLAGWT
                     IAFQDFKPAKGFASAWVGIKHFVDLFTDNVFRNVLRNTLAMSFINLVFNFTCAIGLAL
                     LLNEVRNRGVKRFAQTVSYLPHFLSWVVVAGLVSSMLASDGVFNHILTTLGIAKTPVV
                     WLGQPNAFWWIVGFSNVWKEVGWNTIIYLAAMAAIDPALYEAAEMDGCGRFSRIRHVT
                     LPGIRPTIVVLLILNLGWIMESGFEIQYLLKNGMNQDFSWSIDIYVVEYGLRLGNYSL
                     ATAGGIFKSVVNLVLLFSANTIASRLGQERLV"
     gene            4247..5179
                     /locus_tag="VFH83_09785"
     CDS             4247..5179
                     /locus_tag="VFH83_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6486699.1"
                     /translation="MLRLGGKRYLDVRSFAADAGFGLFNAVFLGAIVLVTLYPFWNTI
                     VVSLNQAIDTTRGGLYLWPRIWTWQNYKAVWVSANIPHALFISVARSVASTVCNLFLT
                     TMLAYALGRREYVLRRPLTLIVVVSLYVNAGLFPNYFLIRNLHLNNTFWVYVVPTMIN
                     GFNFIVIRTYIKTIPESIFESARIDGTSDFAILLRFVLPLSKPVLATVALFVAVGAWN
                     AWFDTLLYASGKPKLFTLQYQLMALLQASMNQSRSTADLNAAGLTANAAKTIVTPASI
                     RAAITIIASAPILLVYPFLQKYFVVGLNVGSIKE"
BASE COUNT          956 a         1783 c         1669 g          984 t
ORIGIN      
        1 ctccgatgat ctcctgcact gggaatccag agagctggac tcggcctggc ccggcggtga
       61 gtgcgcggtc gcctttgcga gctaccatcc cacgaaccgg gaccacctcg ttctgatgac
      121 cggcggcaac cacaccgggc acttctacgc cataggggag gtgctgttct cgctccgtga
      181 tcccgagaca ccgatcgcct ggctgccgcg gccggtcctc gcggcggacg ccacgatccc
      241 ttacgaagac gggtgctcgg cggagcctcc gcaccggccg gtctcctact ggcgtgatac
      301 ggtgttcgtc tgcgggatga cgctcttcaa ggggagttgg ctcgcctact atggcggcag
      361 cgagtactac acctgccttg ccacggcgcc ggcgcgcgga gggggaacgg aatgctcatg
      421 aacacgcagc gcgagctgtt cgaaaagtcc aaggtcgtct acgagagtgc cgcgctgagc
      481 ttccggggcg tggagggctt cgacgtctac aacacatcgg tcccctttgt ctggaagggc
      541 cgatcgttcc tcttgggccg ggtggagcgg cgcgccgact gggcgcgctc gtgggttcgg
      601 ctctttgccc ccaccggacc ggatgagtgg accctggtgg cgaacgccat gatctatccg
      661 ctggaggacc ccttcatcgc ctggatcggc ggggagctgg tcatgggcgg gacccacgtc
      721 cggtaccggg cgggcgagat cgatacgctg tacacctact tctaccgggg gagcgagctg
      781 cacgaccttc gctacttcac gaccggcccg gacgccatga aggacatccg cctggtgcag
      841 cttgccgacc agcggatcgg cgtgttctcc cgcccgcgca accaggagat cgtgcgccgg
      901 ttcgggaccg aggcgcagat cgggttcacg gtcatcgatc ggctggagga cctgacgagc
      961 gacgtcatcg agcaggcggc atacataccg ggtctgtttg cgcaggggga gtggggcgga
     1021 tgcaaccagg cgctgcttct cgagaacggt cggatcggca tcatcggcca caagtcctac
     1081 acgcacgggg ccgcggacgg cgccaagggc atggtctatg tgaacgtgtc cttcgtgttc
     1141 gatccggcga agcgcgaggc ctctgatctc aagatcatcg ggacgcgcac ctgctacccg
     1201 caggggccgg cgaagcggcc ggagctgggg gactgtgcgt tcacgtcggg gatcgtgatg
     1261 cgcccggacg gaagggcgga tctctacagt gggattggag acaccggaga aggacgcatc
     1321 acgatcgact acccctttcc cgctcgcatc gtgtcgccat gacggggcgc cggagcctgg
     1381 cgtcgccggt gctcgccgga tgagccgcct gcgcttcacc tatgcaacaa tagggaaaaa
     1441 gtcctattac ggcctggatc gccccggttt atcctgacgc ccatccacgc ggctgtgagc
     1501 cgcggcgact caggtgatcg gaggtagaca tgagaagaca tggagtcccc agggtcctcg
     1561 tggtcctggc cgtctttgcc ggccttgccg tctcggcggg cgcccaaacc gccgtcgaca
     1621 agctgccggt gaccaagctc ccgcccatca cgatctcggt cttcacggtc aacacccagt
     1681 tcccgcttcc cccggatgac aaccgggcct accagctcat caaagacaag ctcggggtca
     1741 cgttcaagtg ggacatcgcc gtcggggagg cggaccagaa gatcggcgtc atgatcgcag
     1801 ggcaggatta cgacgacctc ctgcacgtca actcgcccaa gttcatcgac gccggcgccc
     1861 ttatccccct ggaagacctg atcgacaagt acgcgccgca cctcaaggcg cactacgagt
     1921 cggatccggt gacctgggcc aagatgcacg agaaagacgg gcacgtgtac acgctcctgg
     1981 actacggagt catcgacaat ggcccgacgg ctaccgacta ctacggcgcc gcgatgttca
     2041 tccagaaggc cgtgatgagg gagtccaact tcccgaagat cgcgaccatc gatgagtact
     2101 tcgacctcat ttcccgctac aaggcacggc acccgaccac cgccgacggc aagccgacca
     2161 tcggcttctc catcctcacc tatgactggc acaagttcga cctggtgaac ccgccgccgt
     2221 tcctggccgg gtaccccaac gacggggacg gcattgtgga ccccgtgacc cacaagtacc
     2281 gcctgcagta ctacggcccg gaggcccacc gctggttcaa gctcctcaac cagatgaacg
     2341 acaagggcct cgtcgaccac gattccttcg tcgacaacta cgaccagtac ctggccaagc
     2401 tggcaaacgg ccaggtcctc ggcgtccatg accagtactg gcagttccag accgcctcgc
     2461 agaccctcgt ctctcagaac cggatctggg acaccatgat gcccctgccc attgtcttcg
     2521 acaagtcgat acggcctcac tggcgcgact ccccgcttcc caacctgcag cgcggctacg
     2581 ggatcagtat cgaggcgaag gaccccgttc gcatcatcaa gttcctcgac gagcagtacg
     2641 atccggcgtg gtacaaggtc ttccagtggg gtctggaggg cgtggactac agcatcgaca
     2701 acgacgggca gccggtgatg accccgctgc agcgccagca ctacgatgat gtgacctggt
     2761 ggctgcacaa cctcgcggag ctgtggtggc aagaggcgcc gaagtacgag ggccacttca
     2821 cccggggcgg cctggcggcg cccctgcggg acaacccgaa ggaatacttc gcctcgctgc
     2881 atcccgagga cgcgcagatc ctcaaggcct acggcgcgac gagctggtcc gacctgatgg
     2941 atcacaacac gccgcccaac ccgccgtggt acccggcctg gcagatcacg cccagcgacg
     3001 gtacccccgc gcagctcgct tttgcaaagg cgcagcaggt gtacgtgaaa gacctgccca
     3061 aggtcatcct ggcgaagccc gctgacttcg agaaggagtg gcagagctac gtcaacgacc
     3121 tcatgcaggc aaacgtcaag gtgttcgagg acttcatcca ggcggggatc gacgaccgca
     3181 tggcccggta cggccagaag aggtagggtc tccctgcgct gatctccagg ggcgcggggc
     3241 caggcctgtt gcggcacccg gctgcggtac tccgcagccg gccttggctc cgcgtcttgc
     3301 cgggcgggga ggaatggccg atgtccagca tcgttcccgg gaagcagggc ttctggagca
     3361 ggctcgcggc gcagcggcag cttgcctgga tgtcggtgcc cctcgtcctg tatgtgctcc
     3421 tcttcaccta cgtccccctt gccgggtgga ccatcgcctt ccaggacttc aagccggcca
     3481 agggcttcgc ctcggcgtgg gtcggcatca agcacttcgt ggatctcttc acggacaacg
     3541 tgttcaggaa tgtcctgcgc aacactctgg ccatgagctt catcaacctg gtgttcaact
     3601 tcacctgcgc catcggcctg gcgctcctgc tcaacgaggt gaggaaccgg ggcgtgaagc
     3661 gcttcgccca gaccgtctcc tacctcccgc acttcctctc ctgggtcgtc gttgccggac
     3721 tggtctccag catgctggct tcggacgggg tcttcaacca catcctgacg acgctgggga
     3781 tcgcaaagac cccggtcgtc tggctgggac aacccaacgc gttctggtgg atcgtcgggt
     3841 tctccaacgt ctggaaggag gttggctgga acacgatcat ctaccttgcg gcgatggcgg
     3901 ccatcgatcc tgccctctat gaggcggcgg agatggatgg ctgcggtcgg ttctcccgca
     3961 tccgccatgt cacccttccc ggcatccggc cgaccatcgt ggtgctcctg atcctcaacc
     4021 tgggatggat catggagtcg ggcttcgaga tccagtacct gctcaagaac gggatgaacc
     4081 aggacttctc ctggagcatc gacatctacg tggtggagta cgggctgcgg ctgggcaact
     4141 actcgctggc cacggcggga ggcatcttca agagcgtcgt caacctcgtg cttctcttca
     4201 gcgcgaacac gattgcctcc cggctcggcc aggagaggct ggtctgatgc tgcgactggg
     4261 cgggaagcgc tacctggacg tccgcagctt cgcggcggat gccggcttcg ggttgttcaa
     4321 cgcggtcttc ctcggggcga tcgtgctggt gaccctctac cccttctgga acacgatcgt
     4381 cgtctcgctc aaccaggcga tcgacacgac ccgcggcggc ctgtacctct ggccccggat
     4441 ctggacgtgg cagaactaca aggcggtctg ggtctccgcc aatatccccc acgccctgtt
     4501 catctcggtg gcgcggtcgg tcgcctccac cgtctgcaac ctcttcctga ccacgatgct
     4561 ggcctacgcg ctgggccgcc gggagtacgt gctgcgccgg ccgctcaccc ttattgtcgt
     4621 ggtctccctg tacgtgaacg ccggcctctt ccctaactac ttcctcatcc ggaacctcca
     4681 cctgaacaac acgttctggg tctatgtcgt gcccaccatg atcaacgggt tcaacttcat
     4741 cgtgatccgg acctacataa agacgatccc cgagagcatt ttcgagtctg cgcggatcga
     4801 cgggacaagc gacttcgcca tccttctcag gttcgtgctg ccgctgtcca agcccgtcct
     4861 cgcgacggtg gccctcttcg tggcagtcgg cgcgtggaac gcctggttcg acaccctgct
     4921 ctatgcgtcc gggaagccaa agcttttcac cctgcagtac cagctcatgg cgctcctgca
     4981 ggcgtccatg aatcagtcga ggagcacggc ggacctcaac gcggccggcc tgacggccaa
     5041 cgcggcgaag acgatcgtca cacccgcctc gatccgggcg gccatcacga tcatcgcgtc
     5101 cgccccgatc ctcctggtct accccttcct ccagaaatac ttcgtggtcg ggctcaacgt
     5161 cgggtcgatc aaggagtagt acgacctgct ctctggctat ggcacccggt tgtgcatcgt
     5221 cgcggtcggg tttaccgtgg gagccgagga agcttggcgc tagcctggag tgaactggta
     5281 tggagttcaa ggacgactgg gaaaaagcga ggcagcgctt cgaggccttc tgggcaggag
     5341 agctcctgga tcgatgctgc ctggcggtga cctccccgcg ggccggatcg cg
//
LOCUS       DASRZS010000402         4357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2143113, whole genome shotgun sequence.
ACCESSION   DASRZS010000402 DASRZS010000000
VERSION     DASRZS010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2143113"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VFH83_09790"
     CDS             complement(<1..599)
                     /locus_tag="VFH83_09790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HET6486700.1"
                     /translation="MIQRTADVVVIGGGSAGLAAALRVRQEGHSVVLIEREPFLGGIL
                     LQCIHSGFGLHEFGEDLTGPEYAERFVRPTLAGGIDVYTDTTAMEISRDEAGPWRKTV
                     LALSLRFGVMRFSCRAVILAMGCRERNRGGVPIAGSRPAGIFTAGLAQRLVNVEGCIP
                     GRRVVIVGSGDIGLIMARRLSLVGCQVLGVIEIQPYPSG"
     gene            complement(596..2044)
                     /locus_tag="VFH83_09795"
     CDS             complement(596..2044)
                     /locus_tag="VFH83_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6486701.1"
                     /translation="MSAPRVDVAIIGAGVSGAAIARRLSSYRLSIALLERRADVSFGV
                     SKANSGIIHAGFHHAPTSLKARLEVEGNRAFDRLKAELDFPFRRVGIVVAAFTPEELK
                     IVERLYQQGVDNGVSDIEIVGKDRMLSLEPALSRDVLGGLHAPSGGIIEPYRFVFALV
                     ESAAANGVQLHTSWEASAARDLGDRWEIASRTGERLEARWVVNAAGLFADRVSGLFGA
                     ESFTIRARKGEEYILERGAAGAPTRVIFPVPTRTSKGVLVIPTVEGTVMVGPTAEETD
                     DKEDFSTTPENLKRVFELASRMVPAISLRSIITSFAGLRPTLPGDDFYIEASKAVPRF
                     IQVAGVQSPGLTASPAIAERVKDILAAQGLTLAEKPDWKPGLPRSERIRDRSAWDLDR
                     LAAQDPAYAHVVCRCECVSEAEVVEAIRKGHHTLDGIKYYTRAGMGRCQGGFCTYRIM
                     RLIARETGMDLTEVTKSGPGSELLVRKLGGQP"
     gene            complement(2041..2373)
                     /locus_tag="VFH83_09800"
     CDS             complement(2041..2373)
                     /locus_tag="VFH83_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET6486702.1"
                     /translation="MKLLFVVLNSADRLEEVLEGLIEVGVTGATVVDSVGMGHIIEGV
                     PLFAGMRNIFRGAKPRNNMIFSVLRDDQANEALQVLDKIMGCTGGHGLGIAFTLPIDN
                     AIGFCKEP"
     gene            complement(2373..2840)
                     /locus_tag="VFH83_09805"
     CDS             complement(2373..2840)
                     /locus_tag="VFH83_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET6486703.1"
                     /translation="MSMKLTQYIRSDCFLPDLSGRTREGALREIVHAVAVKGLIKEEQ
                     EVYARLMERENIQSTAVGNGIAIPHCFTDDVPDLVITVARSMAGLEFDSFDGKPTQVV
                     FLLMGNRQEHSLHLKALARIARLIKSTAFIEKIIASETVEDMVRAFEQEEAKI"
     gene            complement(2946..4022)
                     /locus_tag="VFH83_09810"
     CDS             complement(2946..4022)
                     /locus_tag="VFH83_09810"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET6486704.1"
                     /translation="MSDFALPEGAAIKPIRWYRSPLTKEALRSLTQRSDLRGLLQTVP
                     FLLLLAVTGTAAVLAVGRLPAIAVIAILFVHGTFYGFLVAGWHELSHGTVFKTRALNR
                     IFLRIFSFLGWHDDVLYKQSHVRHHSSTLHPPDDLEVVLPVRLHVWAFLRSAIIDPVD
                     LYRVLANAVRHSLGRLKRGGVSSFGEHPSGEWENKLFPAADPEARSRLFTWARLTLLG
                     HAAVCAVGIGTGLWILPVVITFGRFYGGWLVWLCGNTQHTGLRDNVDDFRLCCRTIRI
                     NPFLQFLYFHMNYHADHHMYAAVPCYNLSRLHQAIIDDMPRASKGLVGAWGEIIEILR
                     KQEKDPSYQHVLELPAARQQPSTA"
     gene            4155..>4357
                     /locus_tag="VFH83_09815"
     CDS             4155..>4357
                     /locus_tag="VFH83_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6486705.1"
                     /translation="MEARRHAPTTSSDVVYRELFNRILMGKFKTESRLKEKEIAEEFE
                     VSRGPVREALRELAQDGLVEITA"
BASE COUNT          781 a         1412 c         1428 g          736 t
ORIGIN      
        1 atgccggagg gataaggctg gatctcaatg accccgagca cctggcagcc caccagggag
       61 agacggcggg ccatgatgag accgatgtct cctgagccta cgatcaccac ccggcggccc
      121 gggatgcagc cctctacgtt caccaggcgc tgagccaggc cggcggtgaa gatcccggcg
      181 gggcgagagc cggcgatggg cacgcccccg cggttgcgct cgcggcaccc catggcaagg
      241 atgacggccc ggcaggagaa ccgcatcacg ccgaagcgca gggacagcgc cagcacggtc
      301 ttgcgccacg gccccgcttc gtcccggctg atctccatgg ccgtggtgtc cgtgtatacg
      361 tcgatgccgc ccgccagcgt cggccgaacg aaccgctcgg cgtactcggg ccccgtgagg
      421 tcctcaccga actcgtggag gccgaagccg ctgtggatgc attgcagcag gatgccgccc
      481 aggaacggtt cgcgctcgat cagcacgacg gagtgccctt cctggcggac ccggagggcc
      541 gccgccagac cggcgcttcc gccgccgatg accacgacgt ccgcggtgcg ctggatcacg
      601 gctggcctcc gagcttgcgg accaggagct cactccccgg accgctcttg gtgacctccg
      661 tcaggtccat tccggtttcc cgcgcgatca gccgcatgat gcggtaggtg cagaacccgc
      721 cctggcatcg ccccatgccg gcccgcgtgt agtacttgat gccgtccagg gtgtggtgtc
      781 cctttcggat ggcctcgacc acctcggcct cggatacaca ctcacaccgg cagaccacgt
      841 gggcgtacgc cggatcctgc gcggccagac ggtccaggtc ccaggcgctg cggtcgcgga
      901 tccgttcgga tcgaggcagg ccgggcttcc agtccggctt ctccgccagc gtcagcccct
      961 gggccgcgag gatgtccttg actcgctcgg cgatggcggg agacgccgta aggcccggcg
     1021 actgcacccc tgccacctgg atgaaccgcg gcacggcctt ggatgcctcg atgtagaaat
     1081 cgtcccctgg gagcgtggga cgcaatccgg caaaggaggt gatgatgctt cgcagcgaga
     1141 tggccggcac cattcgtgag gccagctcga acacccgctt gaggttctcc ggcgtggtgg
     1201 agaagtcctc cttgtcgtcg gtctcctccg cggtcggtcc caccatgacg gtcccttcca
     1261 ccgttgggat gaccaggacg cccttggagg tgcgggtggg cacggggaag atcactcgcg
     1321 tgggcgcgcc ggctgcgccg cgctccagga tgtactcctc accctttcgg gcccggatcg
     1381 tgaagctctc cgccccgaag agaccggata ccctatccgc aaagaggccc gccgcgttca
     1441 ccacccaccg cgcctccagc cgctcgccgg ttcgggaggc gatctcccag cggtccccca
     1501 ggtctctggc ggcggaggct tcccagctcg tgtggagctg gacgccgttg gcagcggcgg
     1561 actccaccag ggcgaagacg aaccggtacg gctcgatgat gcccccgctg ggcgcgtgca
     1621 gaccacccag cacgtcacgg ctgagggccg gctccagcga cagcatgcga tccttgccaa
     1681 cgatctcgat gtccgaaacg ccgttgtcca cgccctgctg gtagaggcgt tcgacgatct
     1741 tgagctcctc cggcgtgaag gcggcaacca cgatgcccac gcggcggaag gggaaatcca
     1801 gctccgcctt gaggcggtca aaggcgcggt tgccctccac ttccaggcgc gccttgaggg
     1861 aggtgggggc gtggtggaac cccgcgtgga tgatccccga gttggccttg gacacgccaa
     1921 aggacacgtc ggcgcgacgt tccaggagag cgatgctcag gcgataggag gagaggcgtc
     1981 gagcgatggc cgcgccggag acaccggctc cgatgatcgc gacgtcgacg cgcggggcgc
     2041 tcacggctcc ttgcagaagc cgatggcgtt gtcgatggga agagtgaagg cgatccccag
     2101 gccgtggcca ccggtgcagc ccatgatctt gtccaggacc tgcagcgcct cgttggcctg
     2161 gtcgtcccgc aggacggaga agatcatgtt gttgcgcggc ttggcgcctc ggaagatgtt
     2221 ccgcatcccg gcaaacagcg gtaccccttc gatgatgtgt cccatgccga cggagtccac
     2281 cacggtggcg ccggtgacgc caacctcgat gagaccctcc aggacctctt ccagccggtc
     2341 ggccgagttc aataccacga aaaggagctt catcagatct tcgcctcctc ctgctcgaat
     2401 gcccggacca tgtcctccac cgtctcggaa gcaatgatct tttcgatgaa cgcggtgctc
     2461 ttgatcaggc gcgcgatccg cgccagcgcc ttgaggtgaa ggctgtgctc ctggcgattg
     2521 cccatgagca ggaacaccac ctgggtcggc ttgccgtcga aggagtcgaa ctccaggccg
     2581 gccatggagc gcgccacggt gatcaccagg tccggcacgt catccgtgaa acagtgcggg
     2641 atggcgatcc cgttccccac ggcggtgctc tggatgttct cacgctccat gagccgggcg
     2701 tagacctcct gctcctcctt gatcaggccc ttgacggcga cggcgtgcac gatctcccgc
     2761 agggctccct cccgcgtgcg cccgctcaaa tccgggagga aacagtctga cctgatgtat
     2821 tgggtgagct tcatcgacat ggcgttctcc agcctggatt gcctgggtga ggctcccaat
     2881 gtaaccactc ccctcgggac ggtgtcaagg caagagattg ccgtatcggt ggcgcggtgc
     2941 ccggctcagg cggtcgatgg ctgctgccgc gccgccggta gctccaggac gtgctggtac
     3001 gaagggtcct tctcctgctt ccggagtatc tcgatgatct ctccccatgc cccgacgagg
     3061 cccttcgagg cgcgtggcat gtcgtcgatg atcgcctggt ggaggcggga caggttgtag
     3121 caagggaccg cggcatacat gtggtgatcc gcgtggtagt tcatgtggaa gtataggaac
     3181 tgcaggaaag ggttgatccg gatcgtccgg cagcagagcc ggaaatcatc cacgttgtcc
     3241 cggaggccgg tatgctgggt gtttccgcag agccagacga gccatccccc gtagaaccgg
     3301 ccgaacgtga tgacgaccgg caggatccac aggcccgtac cgattcctac cgcgcagacg
     3361 gcggcatgcc cgagaagcgt cagccgcgcc caggtgaaaa ggcgactccg cgcttccggg
     3421 tccgctgccg ggaacaactt gttctcccat tcgccagaag ggtgctcccc aaaggaactg
     3481 acgccgcccc tcttgaggcg gcccaggctg tgccggaccg cgtttgccag cacccggtag
     3541 aggtccacgg ggtcaatgat tgccgatcgg aggaacgccc agacgtggag ccgaaccgga
     3601 agcaccactt ccaggtcatc cggtggatgc aaggtgctcg agtggtgccg aacgtggctt
     3661 tgcttgtaca gaacatcgtc gtgccacccc aggaagctga agatcctcag gaagatccgg
     3721 ttcagggccc tcgtcttgaa caccgtgccg tgggagagct cgtgccaccc agccaccagg
     3781 aacccgtaga aagtgccgtg gacgaagagg atggcgatca cggctatggc aggcagacgt
     3841 cctaccgcca ggactgcggc cgtccctgtc acggccagga gcagcaggaa cgggacggtc
     3901 tggagcagac cgcggaggtc actgcgctgc gtcagcgaac gaagtgcctc tttggtcagc
     3961 gggcttcggt accagcggat cggcttgatc gcggcacctt cgggcagtgc gaaatcgctc
     4021 attgcttcta tgatcagcaa ggtcagccat attgtcaaca aaaacggaga aagtgtatgt
     4081 caagtcgaga ctcttgcaga gactgttgtc tgggtatact gtccagaggg accatgattc
     4141 aggagtcaga ccgaatggaa gccaggcggc acgcgcccac tacgtccagc gatgtcgtct
     4201 atcgcgagct cttcaaccgg atcctgatgg gcaagttcaa gaccgagtcc aggctcaagg
     4261 aaaaggaaat agccgaagag ttcgaggtga gccgcggtcc cgtccgggaa gcgcttcggg
     4321 agctcgcgca ggacggtctg gtggagatca cggccca
//
LOCUS       DASRZS010000403         5226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2533186, whole genome shotgun sequence.
ACCESSION   DASRZS010000403 DASRZS010000000
VERSION     DASRZS010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2533186"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1566
                     /locus_tag="VFH83_09820"
     CDS             295..1566
                     /locus_tag="VFH83_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6486706.1"
                     /translation="MTVAVQQPEAGRLTAITNARVFDGERAIDEQTVVLDGTHILAVG
                     GVVPAGATVIDAHGATLMPGLIDSHVHTDMDGLRDALAFGVTTELEMQGHWTAKQRRE
                     IAARHDIADLRSPGMGITPPGGHPSQYMASSSNPLLRLFSHFPMKLFFHFPFVSTPDE
                     AVKFVSKQVRGGADYIKIFIEDGTAIGFPGLKVTSDKTLVAAVEEAHRLGKIAIAHVT
                     TYDGAETAIAGGIDGLAHLFFDRQTTPELTSAIASAGAFVIPTLVTLSTAFGNDASAL
                     AADERVKSRLDRKWLDSLSRSMNVYPSGDMNAAFASVMALHRAGVDILAGSDVSDPIP
                     ILGGLAHGASLHHELQLLVAAGLKPMEALRAATSTPARRFGLTDRGRVVPGARADLVL
                     VEGDPLTHISDTLSIRYVWRGGSVLRPRSLL"
     gene            complement(1766..1850)
                     /locus_tag="VFH83_09825"
     tRNA            complement(1766..1850)
                     /locus_tag="VFH83_09825"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1813..1815),aa:Leu,seq:cag)
     gene            2020..2871
                     /locus_tag="VFH83_09830"
     CDS             2020..2871
                     /locus_tag="VFH83_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HET6486707.1"
                     /translation="MASIRILFIGEIVGKSGVWVVKELLPKIRKERSIDFVIADGEGA
                     TGGFGIGKSHAVYLHKLGVDSITTGECCYYKKDIVDHFARAPYLLRPANYPPKNPGRG
                     FMIYQQNGHKIAVISLLGVAGFKRVHLKNPYTLLPKLLDLIVPETKSIVLDFHAVTTA
                     EKATMFAVADGRLSAVIGTHTKAITSDERVLPGGTAVITDAGRTGSLDSVAGLDPDIE
                     IRKLTTLIHEYSREAWTTLELQGVVLEIGDDGKAAKIERIRESCKAPPQASRDAEVVQ
                     SEGNLES"
     gene            2906..3208
                     /locus_tag="VFH83_09835"
     CDS             2906..3208
                     /locus_tag="VFH83_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486708.1"
                     /translation="MKYQPSLRDRVKGDTIASDVLRKVPQLRSCPRWDRATFLGCVQH
                     TLEFASYDGGLVLYEDRMYYVNRSQIEALRPFVRWNLKKFIRVVPPAATVQDRDEE"
     gene            complement(3229..3630)
                     /locus_tag="VFH83_09840"
     CDS             complement(3229..3630)
                     /locus_tag="VFH83_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486709.1"
                     /translation="MTEPDAASGLRRQLAERGFELLSAPSEPQSGRLHASAGVLRPFS
                     VPPFGTLYALVPVAVALEVGPDGRIVRVDGGEPAARDLDAVQVWLEDLVANNQLEGVD
                     SSAVTPRATHVVFVEGDGRRVVRRKGFRTAV"
     gene            complement(3617..5110)
                     /locus_tag="VFH83_09845"
     CDS             complement(3617..5110)
                     /locus_tag="VFH83_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486710.1"
                     /translation="MKSPSRKKKAKGSVAPSKRAAAPAPSVGGWEDDPGSGPNPTGGI
                     QLSRPVPNLGAQPYALSIANPAAAPAPKRYPVGTAGFRYWAAAEALRRAADFWGSIIP
                     GQHWQPGPVLPVDLEHGTDLNAFYDRVGLRFFKDTVSGRTVYSGESPDVTCHELGHAV
                     LDSVAPGLWDVSSMEVGSFHESFGDMSSLLSALQLPDMRDAALQETAGSIARSSRLSR
                     LAEQLGWAIRTGAPDAVDPDCLRNAANSFSYQDPATLPRTAPASHLCREVHSFSRVFT
                     AAALDALAGMLKASARADADGLLKISQDFARILISAVRASPVVTAFYGQVAAHATAVA
                     EKLFPGTGYSAALNSAFVKHAILSPAGVAGVAAAPTAEAAAAVSSSAVRSEPQSLNVE
                     GYGLGVNEVFVVSPDQAPRFHLPAPRRFAATGEPDHLDAARSYLEDLLSSGRVRAVFP
                     RPAGAGVRPSDPSWRHTHELRSEGGRVVLRRLRVNCGFGTCQRCDRA"
BASE COUNT          981 a         1664 c         1667 g          914 t
ORIGIN      
        1 ggaatggaag cggctacgca gggaacagcc tcgcaggcag aagtgggaag cagtacctga
       61 cgtcctgggc cgaaaccccg cctgagtcgc tcgtttctgg ctcacgggcc tttccggtgg
      121 cagcttccct ttctgcttca agcatgtctt gatcgcaggg gctgattcac gatggatgca
      181 atacgggcgc gaagcactcg tcctgatatc tgaagggcca aaagacgcat accgtcgcgc
      241 ggtgaaggaa agaaacaacg gcccggatag ccatctcaag gcggaggaaa gctcatgacc
      301 gtggcagttc aacaacccga agcgggcagg cttaccgcca ttaccaacgc gcgtgtcttc
      361 gacggggaac gggccataga cgagcagacc gtcgtgctgg acgggaccca tatccttgcg
      421 gttggtggcg tggtgccagc aggggcaacc gtcattgacg cgcacggcgc gaccttgatg
      481 cctggcctca tcgactccca cgtgcatacc gacatggacg gcctgcgtga tgccctggca
      541 ttcggggtca ccaccgaatt ggagatgcag ggacattgga ccgccaaaca acgcagggag
      601 attgccgcgc ggcacgacat cgccgatctt cgctcccctg ggatgggcat cactcctccc
      661 ggggggcacc cgagccaata catggcatcc agcagcaatc cgctcttgag gctattctcc
      721 cacttcccca tgaaactgtt cttccacttt ccttttgtct cgactcccga cgaagcggtc
      781 aagttcgtaa gcaagcaggt ccgtgggggc gcagattata tcaagatctt catcgaggac
      841 ggaaccgcca tcggatttcc cggcctcaag gtgacgagcg ataagaccct cgttgccgcg
      901 gtagaagaag ctcatcgtct cggaaagatt gctattgccc acgtgacgac ctacgatggg
      961 gcggagacgg ccattgccgg aggcatagac gggttggcgc acctgttttt cgatcgccag
     1021 actactcctg aactgacctc cgccatcgct tccgccggag ccttcgtcat tccaacactg
     1081 gtgacgctgt ccaccgcctt cgggaacgac gcctccgccc tcgctgcgga tgagcgcgtg
     1141 aagtccaggc tcgacaggaa atggcttgat tcgctttccc gaagcatgaa tgtctatccc
     1201 agcggggata tgaatgccgc ttttgccagc gtcatggctc tccatagagc gggcgtggac
     1261 atcctggctg gaagcgatgt atccgatccc attcccattc tcggagggct cgcccacggc
     1321 gcaagcctgc accacgaact gcagcttttg gtcgccgctg gccttaagcc catggaggct
     1381 ctacgggcgg ccacctcgac tcccgcgcgc cggttcggac tcactgaccg cggtcgcgtt
     1441 gttcccgggg cacgcgcaga cctcgtgctg gttgaaggtg atcctttgac ccacatatcc
     1501 gacacacttt ccattcgcta cgtttggcgg ggcgggtctg ttcttcgtcc tcggtcactc
     1561 ctctgaacgc gattgggatc cagtgaaccg cccccgtctg ccgaagaccg cgaaccagga
     1621 atgagaaggt tgtcgaagca agaatagtca cagtggtggg agcctggggt actctggggt
     1681 cggcgatcgt tcaggccctc gtgaccgatg cacagccgag gtcaccgtga gagccacatt
     1741 tgccttacga agtaaggacc tgcgttgccc ggaagaggac ttgaacctcc atgcccttgc
     1801 gagcactaga ccctgaatct agcgtgtcta ccaaattcca ccatccgggc gcagttttta
     1861 ccgtaccaat ctggttcgag cttgtcaagg acgcgccact acaggtagat tttcgagtga
     1921 agcaataggg gacgcgcccg ggacggccgg gacggcgtga ctcgcagctt ccggctccgg
     1981 gccgttgatc tcgcttcctt ccatagatat cctcccgggg tggctagcat tcggatcctc
     2041 ttcattggcg aaatcgtggg caaatccggc gtctgggtcg tgaaggagct cctgccgaag
     2101 atccgcaagg agcgctcgat cgacttcgtg atcgccgatg gcgagggagc cacgggggga
     2161 ttcggcatcg gcaagagcca cgcggtgtac ctgcacaagc tgggagtgga ctcgatcacg
     2221 accggggagt gctgttacta caagaaggac atcgtcgacc acttcgcccg ggccccttac
     2281 ctgctgcgcc cggcgaacta cccgcccaag aatccggggc gagggttcat gatctaccag
     2341 cagaacggcc acaagatcgc ggtgatcagt ctcctggggg tggccggctt caagcgggtc
     2401 cacctcaaga atccgtacac cctgttgccc aagctcctgg acctgatcgt gcccgaaaca
     2461 aaatcgatcg tgctggactt ccacgcggtg acgaccgcgg agaaggccac gatgttcgcg
     2521 gtggccgacg gaaggctctc cgccgtgata ggcacgcaca ccaaggccat cacctcggac
     2581 gaacgagtgc ttccgggcgg gaccgcggtc atcacggatg ccggccggac cggaagcctg
     2641 gattccgtgg ccgggctgga cccagacatc gagatccgaa agcttaccac cctgatccac
     2701 gagtactccc gggaggcctg gactaccctt gagctgcagg gcgtggtcct ggagatcggg
     2761 gatgacggca aagccgcgaa gatcgagagg atccgggagt cctgcaaagc tcctccccag
     2821 gcttcccggg acgcggaagt ggtccaatcc gagggaaatc tcgaaagttg atggatagac
     2881 gggccgggtc tacaatggat gtgaaatgaa ataccagcca tcgcttcggg atcgggtcaa
     2941 gggggacacg attgcgtccg acgtgctgcg gaaggttccg cagctgcgct cctgcccccg
     3001 gtgggatcgc gccacgttcc tgggttgcgt gcagcacacc ctggagttcg ccagctacga
     3061 cggcggcctc gtgctgtatg aggaccgcat gtactacgtg aaccgttccc agatcgaagc
     3121 cctgcgcccg ttcgtgcggt ggaatctcaa gaagttcatc cgcgtcgttc cgcccgccgc
     3181 taccgtccag gatcgagacg aggagtaggc cggtcaggtc gaagctcatc agacagccgt
     3241 gcggaacccc ttgcgtctga ccacgcgtct gccatcccct tccacgaaga cgacgtgcgt
     3301 cgcccgcggc gtcacggccg atgagtccac gccctcgagc tggttgttcg cgacaaggtc
     3361 ctccaaccag acctgaacgg cgtccaggtc gcgagctgcg ggctcccctc cgtccacacg
     3421 gacgattctc ccgtcggggc ccacctcgag cgctaccgcc acgggcacaa gcgcgtacag
     3481 ggttccgaag ggcggaaccg aaaacggacg caggaccccg gccgatgcat gaaggcggcc
     3541 ggactgcggc tccgacggcg cgctcaggag ctcgaacccg cgctccgcga gctgcctgcg
     3601 gagcccgctg gccgcgtcag gctcggtcac atcgttggca cgttccaaaa ccgcagttca
     3661 cccgtagacg ccgcaacacg acccttcctc cttccgaccg aagctcgtga gtgtgccgcc
     3721 agctgggatc cgacggccga acgcccgcac cggccggccg ggggaagacc gcgcgcaccc
     3781 gacccgagct caaaagatct tccaggtagc tcctggcggc gtcgaggtga tccggttcgc
     3841 cggttgccgc gaaccgtcgc ggcgcgggaa gatggaaccg cggcgcctgg tcaggggaga
     3901 ctacgaatac ctcgttcacg cccaacccgt atccttccac gttgagggac tgtggctcgg
     3961 aacggaccgc ggaggaggaa accgccgcgg cggcctccgc agtcggcgcc gctgctactc
     4021 ccgccacacc ggcgggagac aggatggcgt gcttcacgaa ggcggagttc agggcagccg
     4081 agtacccggt ccccgggaag agcttctccg cgacggccgt ggcgtgcgcc gccacctggc
     4141 cgtagaacgc ggtcacgaca gggctggcgc ggaccgcgga aatcaggatc cgggcgaaat
     4201 cctgggagat cttgagaagt ccgtcggcgt cggccctggc actcgccttg agcataccgg
     4261 ccagggcgtc caacgctgcg gcggtgaaga cgcgggagaa cgaatgcacc tcacggcaca
     4321 ggtgggatgc aggcgccgta cgcggtagag tggccggatc ctgataggag aagctattgg
     4381 cggcgttgcg aaggcagtcc gggtccacgg catcgggcgc acccgtccgg atcgcccacc
     4441 cgagctgctc ggcaaggcgg gacagccggg aggatcgcgc gatggagccg gccgtctcct
     4501 gcagcgccgc atctcgcatg tcgggaagct ggagcgcgct gaggagcgag ctcatatccc
     4561 cgaaggactc gtgaaacgag cccacctcca tgcttgagac gtcccagagc cccggggcaa
     4621 cgctgtccag gaccgcatgt cccagctcgt ggcaggtcac atccgggctc tcgccggagt
     4681 agaccgtgcg cccgctcacg gtgtccttga agaaccgcag cccgacgcga tcgtagaatg
     4741 cgttcaggtc ggtcccgtgc tccagatcca ctggcaggac cggacccggc tgccagtgct
     4801 gacctgggat gatgctgccc cagaagtccg cggctcttct cagggcctcg gctgcggccc
     4861 agtatcggaa gccggccgtg ccgacggggt aacgcttggg cgccggcgcc gcggcgggat
     4921 tggcgatgga gagggcgtac ggctgcgcgc ccaggttcgg gaccggcctg gagagctgga
     4981 tgccgccggt agggttcggc ccggagccgg ggtcgtcttc ccatccgcct acggatggag
     5041 ccggcgccgc ggctctcttg gatggggcga cacttccctt ggctttcttc ttcctggaag
     5101 gacttttcat tgggggtctc ctgacgattg ggggcccgaa ggcccgtttt cccgggaatt
     5161 tacagggtct ccgacttgcg gtcaatcgac gccaatcgac gcgccgctcc gttgaggcga
     5221 ttgcgt
//
LOCUS       DASRZS010000404         2926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_994501, whole genome shotgun sequence.
ACCESSION   DASRZS010000404 DASRZS010000000
VERSION     DASRZS010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994501"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFH83_09850"
     CDS             <1..313
                     /locus_tag="VFH83_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6486711.1"
                     /translation="CRLAASPMAGHTGELRLTFYPAGLKLAFEDGRLVTAEDWREDHA
                     WGPRSMAGFPPGVFLRLLFGYRSLSELRDAYPDVQTNDEARPLLEALFPPRPTWILPL
                     D"
     gene            complement(321..1625)
                     /locus_tag="VFH83_09855"
     CDS             complement(321..1625)
                     /locus_tag="VFH83_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015733864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HET6486712.1"
                     /translation="MAKITFMGAGSTVFAKNVLGDCLMTPTLAASDIALYDIDPVRLA
                     DSKAMLENINRNAGGAAHIQAYGPTDRRRALTGADYVVNAIQVGGYQPCTVTDFEIPK
                     KYGLRQTIADTLGIGGIFRALRTIPVVLDFAHDMEEVCPDALFINYTNPMAMITGALL
                     KATGIRSVGLCHSVQVCAEWLLKGVGMEYDESVQWKIAGINHQGWLLEISRDGKDLYP
                     EIKKRSLEGRVPREDRVRHEIMHRFGYYVTESSEHTSEYLPYFIKPSYPELIERFNIP
                     LDEYPRRCVAQIAGWERMRSDLVGNKALTHVRTHEYASWIMEAIETNRPYKIGGNVAN
                     ADLISNLPREACVEVPCLVDASGITPCRIGDLPPQCAAVNRTNINVQLLAVEAALTGK
                     REHIYHAAFVDPHTSAELPIDRIVALCDELIEAHGSWLPKYR"
     gene            complement(1637..>2926)
                     /locus_tag="VFH83_09860"
     CDS             complement(1637..>2926)
                     /locus_tag="VFH83_09860"
                     /inference="COORDINATES: protein motif:HMM:NF016563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HET6486713.1"
                     /translation="TLANVLHLFPICSLWAQARPPIGSWCRPEDGLFGLLSGIIDWPD
                     MEVVPDSFYGHVPVICFFPELGLSQMRGHKAYVFPNGAVTWLWGGVSAEAKGGYLITA
                     GTEMATPSPGFQTITNGATYVDMVDRVLMRLGRKELVKEFYPSVKASTLYSMNLRPED
                     GAEGLISAPSGNVDPYNPQRDEGHMLEWFEAVKMFGMVTHIGGIHLAQLVMARRMARE
                     VGDVEFARRCDAWIEAAGRVLEEKMWTGSYYLLYNEPKTGRKSDLVFGYQLDGDWMAK
                     FHGLPGVFPPHRAKTTLETIRRVNAGITSFGAADLAAIDGRQSEGVGYGAVTFFVSEM
                     SILAATYLYEGQREFGLELARRMQVALNQRWGYTWDQPNVLRGDTGEKTLGSQLLQSM
                     FLWIIPAAVLGKNVAEFCEPGGLVDRMIGAARPGGGR"
BASE COUNT          462 a          995 c          931 g          538 t
ORIGIN      
        1 ctgcaggctg gccgcgtccc cgatggccgg ccacaccggg gagctgcgtc tcaccttcta
       61 cccggccggg ctgaagcttg cattcgaaga cgggaggctt gtcaccgcgg aggactggcg
      121 ggaagaccac gcatggggtc cccgatccat ggccgggttc ccgccgggcg tgtttctacg
      181 gctcctcttc ggctaccgga gcctctccga gctgcgagat gcgtatcccg acgtccagac
      241 gaacgacgag gcgcgtccgc tcctggaggc gctcttcccg ccgcggccga cctggatcct
      301 tccgctggac tgaccgactt ctagcggtac ttgggcaacc agctcccgtg cgcctcgatc
      361 agctcatcgc acagcgccac gatcctgtcg atcggcagct ccgccgaggt gtgcgggtct
      421 acgaacgcgg cgtggtagat gtgctcgcgc ttgccggtga gggcggcctc cacggcaagg
      481 agctgcacgt tgatgttggt gcggttcacc gccgcgcact ggggagggag atccccgatc
      541 cgacaaggcg tgattccgct ggcatccacc agacagggca cctccacgca cgcctcccgc
      601 ggaaggttgg agatgaggtc cgcgttggcc acgttgccgc cgatcttgta gggacggttg
      661 gtttcgatcg cctccatgat ccaggaggcg tattcgtggg tgcggacgtg ggtcagcgcc
      721 ttgttcccca cgaggtcgga ccgcatccgt tcccagcccg cgatctgggc aacgcagcgg
      781 cgcgggtact cgtcgagggg gatattgaac cgctcgatca gctcgggata ggacggcttg
      841 atgaagtacg gaaggtattc cgaggtgtgc tcgctggact ccgtcacgta gtagccgaac
      901 cggtgcatga tctcgtgccg gacccggtcc tccctgggca ccctgccctc cagggagcgc
      961 ttcttgatct cggggtacag gtccttgccg tctcgcgaga tctccaggag ccagccctgg
     1021 tggttgatcc cggcgatctt ccactggacg gactcgtcat actccatccc gacgcccttg
     1081 aggagccact cggcgcagac ctgcacgctg tggcacaacc cgaccgagcg gatcccggtg
     1141 gccttgagaa gggcgcccgt gatcatcgcc atcgggttcg tgtagttgat gaacagggcg
     1201 tcggggcaaa cctcttccat gtcgtgggca aagtccagga ccacgggaat ggtccgtaag
     1261 gcgcggaaga tcccgccgat tcccagggtg tcggcgatgg tctggcgaag cccgtacttc
     1321 ttggggatct cgaagtccgt cacggtgcac ggctggtagc cgccgacctg gatggcgttg
     1381 accacgtagt ccgctccggt gagcgccctt cggcgatccg tgggcccgta ggcctggatg
     1441 tgcgccgccc caccggcgtt gcgattgatg ttctccagca tcgccttgga gtccgccaga
     1501 cggaccggat cgatgtcgta cagggcgatg tcgctggcgg ccagcgttgg ggtcatcagg
     1561 cagtccccca ggacgttctt cgcgaacacc gtgctccctg cacccatgaa cgtgatcttc
     1621 gccatgatcc tctcctctat cgtccgccgc ccgggcgagc ggcgccgatc attcggtcca
     1681 cgagcccgcc gggctcgcaa aactccgcga cgttcttccc aaggacggcg gccgggatga
     1741 tccacaggaa catgctctga aggagctggc ttcccagggt cttctccccc gtgtcccctc
     1801 ggagcacgtt gggctgatcc caggtgtatc cccacctctg gttcagggcc acctgcatcc
     1861 ggcgcgccag ctcgagcccg aactcccgct gcccttcgta caggtacgtg gcggccagga
     1921 tgctcatctc cgagacgaag aacgtgacgg ctccgtaccc tacgccctcg gattgccttc
     1981 cgtcaatggc ggcgaggtcc gcggcgccga agctcgtgat cccggcgttg acgcgccgga
     2041 tcgtctccag cgtcgtcttg gcccggtgcg gcgggaatac cccggggaga ccgtggaact
     2101 tcgccatcca gtctccgtcg agctggtacc cgaacaccag gtccgacttc ctcccggtct
     2161 tcggctcgtt gtacaggagg taatagcttc cggtccacat cttctcttcc agcactcgac
     2221 cggccgcctc gatccacgcg tcacaccggc gcgcgaactc cacgtccccc acctcccgcg
     2281 ccatccgtct ggccatcacg agctgcgcga ggtggatccc gccgatatgg gtgaccatcc
     2341 cgaacatctt cacggcttcg aaccactcca gcatgtgccc ctcgtcccgc tgagggttgt
     2401 acgggtccac gttgccgctc ggcgcgctga tgagcccttc cgccccgtcc tccggccgga
     2461 ggttcatgct gtagagggtg ctggccttta ccgacgggta gaactccttg accagctcct
     2521 tgcgccccag ccgcatcaac acccggtcca ccatgtccac gtaagtggcg ccgttggtga
     2581 tggtctggaa cccgggagac ggggttgcca tctcggtacc cgcggtgatg aggtagccgc
     2641 ccttggcttc cgcggacact ccaccccaga gccaggtcac cgcgccgttg gggaagacgt
     2701 acgccttgtg gccgcgcatc tgcgagaggc caagctcggg gaagaagcag atcacgggca
     2761 cgtgcccgta gaagctgtcc gggacgacct ccatgtccgg ccagtcgatg atccccgaga
     2821 gcagcccgaa gagcccgtcc tcggggcggc accacgagcc gatggggggc cgcgcctgcg
     2881 cccacaaact gcagatgggg aagaggtgca ggacgttggc cagcgt
//
LOCUS       DASRZS010000405        10650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3681931, whole genome shotgun sequence.
ACCESSION   DASRZS010000405 DASRZS010000000
VERSION     DASRZS010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10650
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3681931"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFH83_09865"
     CDS             complement(<1..212)
                     /locus_tag="VFH83_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486714.1"
                     /translation="MRFGHHPHPVALVQDGGRLVPKLEGGRCLPSRRSGKVLPGPGTI
                     GSATSGSATSGSTVTGSTVFSGARHGS"
     gene            279..3635
                     /locus_tag="VFH83_09870"
     CDS             279..3635
                     /locus_tag="VFH83_09870"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET6486715.1"
                     /translation="MIEQRPVGATAFQVEIRDAVSSDWNLVQRFDGGGKRLPPRVLCT
                     LPGRTVAGVRIAEIQGPAAIRSVGVFSDSLPPATRLASDANGNIIGVVCDGHGYAPCP
                     GAAVTLRGQSAAGSWSSQAHADEWGLFTVPMPLGLRGTVRVLVDVNGKTASSRLRAED
                     LQYGLTPTSFASRGTVLGEGWRFAGDPPSGFQKPPFKDSGWSPIRVPAHFAMEGFRSL
                     SGVGGYRTRFALATAPGSERLKLLFDGVYSGAEVWVNGIRVAYHEGGALPFEADITDA
                     ARKGTNLLAVRVTEHTLVSDHLDRMSYYANFPLAGIMRKVTLFRVPLLHIGALVVETT
                     FDGRFQDATLDIRAAVLNESGNPTAAHLSARLLDPSGKEAGALGEPVQVEAPAWQRVE
                     TKASVEVRAPRGWDAEHPNLYTLVLELKQGSRVTQRLEKRIGFRQTDIQGARILINGS
                     PVKIRGTCRHDAHPLMGRAITEQVARRDLMMIKEANLNSVRTSHYPPIPELLDIADEI
                     GIYVEDEGSFCWAEGTDDLRNTPRIMQMNAELLARDRNHPSVFMWSVCNESSFGYGFR
                     ASHRWVRAADPRRPTSAATGADLEIATHHNPITVERIREHEDTTAPLLFDEAWCIYQG
                     IFGDVAEMWVDPGIRDYYVDPLPPIYEEMMRSRVTQGSQIWCWSDDIFCVPHMGLEYG
                     RGMTKSHYVETTYQLADRGLSGDAPWGVIDGWRRPKPEYWIIRKLFSPVRITEAPLRA
                     TAPGIFRIPVRNDFDHTDLSELSFRWECAGRRGTAKVSARPHGRATLVLPAPQGARSG
                     TELLLVASDASGRVIDEYRFPIDREVTAPPFPPPVASRPLAIQEENLLATRSVRVVGT
                     DLDLDFAIEDPTAYWNHGGGHLRRCVAFGRPLLLEAPQVHLLPSAAALSPAPDRLSWK
                     ATELRSERSGRNAVVRVRGTYDRFDAEYEWTISPSGEIEIRSTATWNGEETLAREIGL
                     RFSVPLPCEHLEWCRKAEWRSYPADHIGRPRGRARARAGQAARPLPPTWSWSEDTTPM
                     GTNDFRSTKRHVHWASIRYEGGPGVLVISDGTQHVRAMVEPDRIAVHVLDWYGGTNAG
                     LEEWYRNYGKGRPVRSGEAITSSLRLRLIP"
     gene            3632..6139
                     /locus_tag="VFH83_09875"
     CDS             3632..6139
                     /locus_tag="VFH83_09875"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET6486716.1"
                     /translation="MSGPVNLARTARVSVCCAQERGPAALAVDGDPRTCWRSGHHEGG
                     DGYWIELEWSMAVTIRQVGVRQRDDCLTRLSIQIWDGEDWETLATLGNAWDALPRYVY
                     REIPAWTTTRLRLWNLRSSPLYGTIEIAEVEVFEQPVDPPLNWPVLLPEMWPAAADLA
                     ERRGYAERRRAEWRQGFDDGDYRRPDLRWYQEAVTEYFAFVFDRRIYQPDAGWRILPF
                     LEEMSQEIGLIDILVLWQAYPRIGNDPRNQFDFYRELPGGVEELRRQVRAARERGTHV
                     FIDYNPWDTGTRREGVSDAEALGRIAAEMGIDGIFLDVSSDAGNEFRDAVDRHRPGVV
                     FAPEGLPREWGMRHTTGGWVQIYPRLKRLSAVLTPRFVEPRFSARWIDRHVLDNRDYL
                     SRALFNGCGLVVWENIFGYFKPWSAADKAGCREVASILRRFKDNFASPDWVPFVPTRS
                     PEVGANWWPARECSLWTLVNEGERPVDGPVLPVGDARPARFWDVRAGRELHPERVAGE
                     LCLPIHLGARGAGCVQTFFAPDRTGYSIPSAARAPEELAFTRSADLRPAPVTPVGRGL
                     PQPADMVRVSGGPFLFKAEHRDREGACYDDVETWDRHDSDGQDWGAGTAWVWTEHPPH
                     PVVVDDFLIDRTPVTKSAYRGFLRATGYRPSFLERFLDDWSRPPGDPERMDPAAWEPP
                     QGQGNHPVVYVDLDDARAFARWAGKRLPTEEEWQYAAQAGVADRAWPWGHLWEGGRCN
                     EALGYSTPVDAFPAGANPLGILDLVGNVWQLTESERNDGITRYGILKGGSFYKLTQSR
                     YYMDGGPQPLHRHAKFLLMYPGLDRTSTIGFRCARDL"
     gene            6173..6913
                     /locus_tag="VFH83_09880"
     CDS             6173..6913
                     /locus_tag="VFH83_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HET6486717.1"
                     /translation="MAAPDPYKLAERFRKFLRVTDVVDGLDAVGRADLTLMDPSIRPL
                     WMGMRFWGPAVTMQVVPANRRMEPLDRADALRQHEIWARTGGWSDDLSPHIRPGCVIV
                     TSVAGAKECGCWGSNNTLGMEAQGVVGIVTEGTCRDTDEVILQRSPVACRGIGRTIIP
                     GRVELSAVQTTVGCGGVQVRPGDIVGCDWDGVVVVPLEVAEEALYFAVRIAIDDKRGR
                     RRLYERLGKRLDESVDWEEAERYFKDIL"
     gene            6955..7932
                     /locus_tag="VFH83_09885"
     CDS             6955..7932
                     /locus_tag="VFH83_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6486718.1"
                     /translation="MTRSLGNDQSRRRRVRRRPFLIFGILLAIVLVLGVVFVLSDRER
                     LDLSSGVPAGVPGSMVPLSLGNVHYTWSGPSDGPVVVLVNPFSDPAYIWERNVDALTG
                     LGCRVLCYDLYGRGFSDRPRVTYTLDLFVDQLRELLGALAVRVPVRLAGLSMGGEIAA
                     AFTARNPELVDRLCLIDPQAIPITRKTLFPLGVPGVGEYLMTVYVIPFVLSSPVGDFY
                     QPQSLPGWGDRYRPQTTYRGFRRALLSTLRHFPKDYMAAYRRVGELGKPVLLLWGEKD
                     TSIPISNAAGLRAAIPQVEFHPIALAGHLSNYERPDLVNPLLTAFLVRR"
     gene            8005..9021
                     /locus_tag="VFH83_09890"
     CDS             8005..9021
                     /locus_tag="VFH83_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6486719.1"
                     /translation="MDYRRLGTAGLKLSVLSLGSWVTYGRQVDVDAAAACMKAGYEEG
                     VNFFDNAETYEAGKSEEVMGAALAKLGWRRASYVVSTKIYFGMHRGVNEERTLNRKKL
                     REGIDGALKRFGLDYIDLVFCHRPDSETPVEETVWSMHMMVESGKALYWGTSEWSADE
                     LMEAWAIAEKHHLHKPQMDQPQYNLFVRRRVEREYQRLYDRIGLGLTIWSPLAAGLLT
                     GKYNAGIPQGARMGLPGYEWLKSDPLQRGVPAGTAGTPSFAALVEAAKRLEPVAKELG
                     ATQAQLALAWCAHNPHVSSVITGASRPEQVKENMKAIALLPRLTPEVVSRIEKAIGGA
                     SDVS"
     gene            complement(9055..9756)
                     /locus_tag="VFH83_09895"
     CDS             complement(9055..9756)
                     /locus_tag="VFH83_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET6486720.1"
                     /translation="MELRIGTSGYSYKEWKGTFYPETLAAAKMLSFYAERLGTVEINN
                     TFYRLPTTKLLSSWASQVPEGFRFVLKVSQKITHFKRLRNVDEETAYLVETAAALGPK
                     LGALLVQIPPNLARDDDRLFAFLNLLPKGMRAALEVRNDSWRDPAVFALLEKHSVALV
                     ISQTDEDPEPALVKTAPWGYLRLRKTSYQPEELTAWSQRIAEQGWDEAFVFFKHEQIA
                     PDLAAKLSAATAGAS"
     gene            9814..10614
                     /locus_tag="VFH83_09900"
     CDS             9814..10614
                     /locus_tag="VFH83_09900"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HET6486721.1"
                     /translation="MKAFVMTDLEGVAGVVSFEEQTRPDTRYGEQAKRLLTAEVNSCV
                     QGLREAGVTDVVVLDGHGDGGIVYEEIHPEARLIHGKPLAEEWRNQMEGMDVAVIVGQ
                     HAMAGVPDGNLNHTQDSRSITYFTLNGRLIGETAQFALFAGSYGVPLIFLSGDEAACR
                     EAQELIPGVTTAAVKKGLGRNSAVSVSAPRSRELIRAGAAAAVARQRTSPLKPLVWPG
                     PYVLEKRFFGTNHLEQYRYLATVNPNITMVDPLTVRIRGDDIRALIYA"
BASE COUNT         1707 a         3488 c         3731 g         1724 t
ORIGIN      
        1 gagccatggc gagcgccaga aaagaccgtg gagccggtga ccgtggagcc ggaggtcgcg
       61 gagccggagg tcgcggagcc gatggtcccg ggccccggga ggacctttcc gcttcggcgc
      121 gatgggaggc atcggcctcc ctccagcttc gggacgagcc gacccccgtc ctggaccagc
      181 gcgaccgggt gtgggtggtg gccgaaccgc aagccgccct ccgcgctccc ccatggctct
      241 ccttgcagtg gagcgcaccg gtccgggtca gcgaggtcgt gatcgagcag cgaccggtcg
      301 gggcaacggc gttccaggtg gagatccggg acgcggtgtc ctcggactgg aacctggtcc
      361 agcgattcga cggcggcggt aagaggcttc ccccccgggt gctctgtacg ctgcccggcc
      421 gaaccgtcgc cggcgttcgc atcgcggaga tccaggggcc cgcggcgatc cgatccgttg
      481 gcgtcttctc tgacagcctt ccgcccgcca cgcgcctggc cagcgatgcc aacggaaaca
      541 tcattggcgt ggtgtgcgac ggtcacggat acgcgccgtg tcctggagcg gccgtgaccc
      601 tgcggggaca aagcgccgcc ggttcctgga gttcccaggc gcacgccgac gaatgggggc
      661 tcttcacggt ccccatgccc ctgggcctgc gggggaccgt gcgggtgctt gtcgacgtga
      721 acgggaagac ggcgagctcc cgccttcggg cggaggactt gcagtacggt ctgactccga
      781 catcgttcgc cagccggggc accgtcctgg gcgagggatg gaggttcgca ggtgatccac
      841 cctccggttt ccagaaaccc ccgttcaagg actccggctg gtcccccatc cgggtccccg
      901 cccacttcgc tatggaggga ttccggtctc tgagcggcgt gggcggatac cggacccggt
      961 tcgcgctggc aacggcacct ggctcggagc gcctcaagct gctcttcgac ggggtctata
     1021 gcggcgccga ggtctgggtg aatggcatcc gggtcgccta ccacgagggc ggggcccttc
     1081 ccttcgaggc ggacatcacg gatgcggcgc gaaaggggac gaacctcctg gcggtccgcg
     1141 tgaccgagca caccctggtc agcgaccacc tggaccggat gagctactac gccaacttcc
     1201 ctctcgcggg gatcatgcgg aaagtcacgc tctttcgagt tcctctgctg cacatcggcg
     1261 ctctggtcgt tgaaaccacc ttcgatgggc gctttcagga cgcaaccctc gacattcggg
     1321 cggcggtgct caacgagtcc gggaatccga ccgccgccca cctttcggcg cgcctcctgg
     1381 atcccagcgg gaaggaggct ggtgcccttg gggagccggt gcaggtcgag gcacccgcat
     1441 ggcagcgcgt ggaaacgaaa gcctccgtcg aggtccgggc gccacggggc tgggacgccg
     1501 agcaccccaa cctctacacg ctggtcctgg agctcaagca gggaagccgc gtgacgcagc
     1561 gcctggagaa gcgcatcggg tttcggcaga ccgacatcca gggcgcgcgg atcctgatca
     1621 acggcagccc cgtcaagatc cgcggcacct gtcggcacga cgcgcacccg ttgatgggtc
     1681 gggccatcac ggagcaggtg gcgcggaggg atctgatgat gatcaaggag gcgaacctca
     1741 actccgttcg cacctctcac tatccgccca tcccggagct cctggacatc gcggatgaga
     1801 tcgggatcta cgtcgaggat gaggggtcct tctgctgggc ggaaggcacc gatgaccttc
     1861 gcaacacgcc gcgcatcatg cagatgaacg cggagctcct ggcacgggac cggaatcacc
     1921 ccagcgtgtt catgtggtcc gtgtgcaacg aaagcagctt cgggtacggc tttcgggcca
     1981 gccaccgatg ggtgcgggcc gcggaccccc gacgccctac gagcgcggct accggcgcgg
     2041 acctggagat cgccacccat cacaacccca tcaccgtcga gcggatccgg gagcacgagg
     2101 acaccaccgc tccgcttctc ttcgacgagg cctggtgcat ctaccagggg atcttcggcg
     2161 atgtcgcgga gatgtgggtg gaccccggga tccgcgacta ctacgtcgac ccgcttccgc
     2221 ccatctacga ggagatgatg aggagccggg tcacccaggg cagccagatc tggtgttgga
     2281 gcgacgatat cttctgcgtg ccgcatatgg gattggaata cggccggggc atgaccaaga
     2341 gccactatgt ggagaccacc taccagcttg cggaccgcgg cctgagcgga gacgcgccgt
     2401 ggggggtgat cgacgggtgg cgccggccca agcctgaata ctggatcatc cgaaagctct
     2461 tctccccggt ccgaatcacc gaggcgccgc tgagagcgac ggctccgggg atcttccgga
     2521 tcccggtgcg caacgacttc gatcataccg acctgtcgga gctgagcttc cgctgggagt
     2581 gcgcgggcag gcgggggacc gccaaggtct ccgccaggcc gcacgggcgc gctacgctgg
     2641 tcctgccggc tccccaaggg gcgcggtccg gcaccgagct cctcctggtg gccagtgatg
     2701 cttccggtcg ggtcatcgac gagtatcgat tcccgatcga cagagaggtc accgcgccgc
     2761 ctttcccccc accggttgcc tcccgccctc tggccatcca ggaggagaac ctcctggcca
     2821 ctcgaagcgt gcgcgtggtc ggcacggacc tggacctgga cttcgccatc gaggacccga
     2881 ccgcctactg gaatcacggc ggcggccacc tccggcgctg cgtcgcattc gggcgcccgc
     2941 tcctcctgga ggcgccgcag gtccacctgc tgccgtccgc ggctgcgctc tccccggccc
     3001 ccgaccggct gagctggaag gcaacggagc ttcggtccga acgcagcggg cggaacgcgg
     3061 tggtaagggt ccgcggaacc tacgatcggt ttgacgccga atacgagtgg accatctcgc
     3121 cttcgggaga gatcgagatc cgctctaccg ccacgtggaa cggggaggag accctggccc
     3181 gggagatcgg gctgcggttc tccgttccgc ttccctgcga gcacctggag tggtgccgaa
     3241 aggcggagtg gcgatcgtac cccgcggacc acatcggtcg tccccggggc cgggcgcggg
     3301 cccgagccgg acaggcggcc cgtccgcttc cgcccacgtg gtcctggtcg gaagacacca
     3361 cgcccatggg gaccaacgat ttccgcagca ccaagcgcca cgtccactgg gcttcgatcc
     3421 gctacgaggg cgggcccggc gtcttggtga tctccgatgg cacccagcac gttcgcgcca
     3481 tggtagagcc ggaccggatc gcggttcatg tcctggactg gtacggcggc accaacgccg
     3541 ggctggaaga gtggtacagg aactacggaa agggccgacc cgtccgttcc ggcgaggcca
     3601 tcacgtccag tctccgcctc cgcctcatcc cgtgagcggc ccggtgaatc tggcccgcac
     3661 cgcgcgggtc tccgtctgct gcgctcagga gcgcggccca gcggccctgg cggtcgacgg
     3721 ggacccccgc acctgctggc ggtccggaca ccacgaaggc ggagatggct attggatcga
     3781 gctggagtgg tcgatggccg tgaccatccg ccaggttgga gtgcgccaac gggacgattg
     3841 tctgacccgg ctctcgatcc agatatggga tggcgaggac tgggagacgc tggcaacgct
     3901 gggaaacgcg tgggacgccc ttccgcgcta cgtctaccgc gagatcccgg cttggaccac
     3961 cacccggctg cggctctgga acctgcggag ctctccgttg tacgggacga tcgagatcgc
     4021 cgaggtggag gtgttcgagc agccggtgga cccgccgctc aactggccgg tcctcctgcc
     4081 ggagatgtgg ccggccgctg cggacctggc ggagcgccgg gggtacgcgg agaggcgccg
     4141 ggccgagtgg cgccaggggt tcgacgacgg cgactaccgt cgcccggacc tgcggtggta
     4201 ccaggaggcg gtgaccgaat acttcgcgtt cgtgttcgac cggcggatct accagccgga
     4261 cgccggctgg cggatcctgc cgttcctgga ggagatgtcc caggagatcg gcctcatcga
     4321 catcctggtc ctctggcagg cgtacccacg gatcgggaac gacccgagga accagttcga
     4381 tttctatcgc gagctgccgg gtggggtgga ggagctgcgg cggcaggtgc gggcggcccg
     4441 ggagcgcggc acccacgtct tcatcgacta caacccgtgg gataccggca cgcggcggga
     4501 gggagtgagc gacgcggagg cgttgggccg gatcgccgcg gagatgggca tcgacggcat
     4561 cttcctggac gtctccagcg acgcgggaaa cgagtttcga gatgccgtgg accgccaccg
     4621 cccgggcgtt gtcttcgcgc cggaggggtt gccgcgcgag tggggcatgc ggcataccac
     4681 cggcggttgg gttcagatct acccgcggct gaagcgcctc tccgcggtgc tgacgccacg
     4741 gttcgtggag ccgcggttct ccgctcggtg gatcgaccga cacgtcctcg acaaccggga
     4801 ctatctctcc cgcgccctgt tcaacggctg cggcctggtg gtctgggaga acatcttcgg
     4861 ttacttcaaa ccctggtcgg ccgcggacaa ggcaggctgc cgggaggtgg cgtcgatcct
     4921 tcgcaggttc aaggacaact tcgcgagccc ggactgggtg ccgttcgttc ccacccgttc
     4981 gccggaggtc ggcgccaact ggtggccggc ccgagagtgc agcctgtgga cgctcgtcaa
     5041 cgagggcgag cggcccgtag acgggccggt cctgccggtc ggtgatgcca ggccggctcg
     5101 cttctgggat gttcgggccg gccgcgagct ccatcccgag cgggtggcgg gggagctgtg
     5161 cttgcccatc cacctgggcg cgcgcggcgc gggctgcgtc cagacttttt tcgccccgga
     5221 ccggaccggg tattcgatcc cgtcggctgc gcgcgcgccc gaggagcttg cattcacccg
     5281 cagcgcggac ctgcggccgg cgcccgtgac acccgttggc cgcggtctcc ctcagccggc
     5341 ggacatggtc cgggtcagcg gcggtccgtt cctcttcaag gcggaacacc gcgaccgcga
     5401 aggggcctgc tacgatgacg tggagacctg ggaccgccac gactcggacg gtcaggactg
     5461 gggagccggt acggcctggg tatggaccga gcaccccccg cacccggttg tcgtagacga
     5521 tttcctgatc gatcggacac cggtaaccaa gagcgcctat cgggggttcc tccgagccac
     5581 ggggtatcgt ccatcgtttc tcgagagatt cctcgacgac tggagccgtc cgccgggcga
     5641 ccctgagcga atggaccccg cggcgtggga gcctccgcag gggcagggaa accatcccgt
     5701 ggtctacgtg gacctggacg acgcacgcgc tttcgcgcgc tgggcgggca agcggcttcc
     5761 cacggaagag gagtggcagt acgcggccca ggcgggcgtc gcggaccgcg cgtggccgtg
     5821 ggggcatcta tgggaaggcg gccggtgcaa tgaggccctg ggctacagca cgccggtgga
     5881 cgcgtttccc gccggcgcca acccgctggg catcctggac ctggtgggga acgtctggca
     5941 gcttaccgag agcgagcgca acgacgggat cacccgatac gggatcctca agggcggcag
     6001 cttctacaag ctcacccaga gccgctacta tatggatgga gggccgcagc cgctccatcg
     6061 gcacgccaag ttcctgttga tgtacccggg gctggatcga acgtccacca tcgggtttcg
     6121 gtgcgccagg gacctgtagg gcccggctgc cgtcctcgga aagggagcgc gcatggcggc
     6181 accggacccc tacaagctcg ccgagaggtt tcgaaagttc cttcgcgtga cggacgtcgt
     6241 cgatggactg gatgccgtgg gacgagccga tctgaccctg atggatccct ctatccgccc
     6301 cctctggatg ggcatgaggt tctggggtcc cgccgtcacc atgcaagtcg tgccggcgaa
     6361 ccgccgcatg gagcccctgg accgcgcgga cgcgctgcgg cagcacgaga tctgggcccg
     6421 gacgggtgga tggagtgacg acctctctcc ccatatccgc cccggctgcg tgatcgtgac
     6481 ctccgttgcc ggagcgaagg agtgcgggtg ctggggatcg aacaacaccc tgggcatgga
     6541 ggctcaaggg gtcgtgggca tcgtcacgga aggtacctgt cgggacacgg acgaggtgat
     6601 cctgcagcgc tctcccgttg cgtgccgcgg aatcggtcgg accatcatcc ccggccgcgt
     6661 ggagctgagc gcggtgcaga cgaccgtagg gtgtggcggg gtacaggttc gcccgggtga
     6721 catcgtgggc tgcgactggg acggcgtggt ggtcgtcccg ttggaggtcg ccgaggaggc
     6781 cctgtacttc gccgtgcgga tcgccattga cgacaagaga ggtcggcgca ggctgtacga
     6841 gcggctgggc aagcggctgg atgaaagcgt agactgggaa gaagcggaac gctacttcaa
     6901 agacatcctg tagattcaaa aacggttccg ttgcgccgtg ggaggacgga aatcatgacg
     6961 aggtccctgg gcaacgatca gtctcgacgc cgccgggtgc gccggcggcc cttcctcatc
     7021 ttcgggatcc tgttggccat cgtgctggtc cttggggtgg tgttcgtcct gtccgaccgg
     7081 gagaggctcg acctgagcag cggcgtcccc gcgggggtgc caggctccat ggtcccgctc
     7141 tccctgggga acgtccacta cacctggagc ggcccttccg atggtcccgt ggtggtcctg
     7201 gtcaatccct tttcggatcc cgcgtacatt tgggagcgga acgtggatgc tctgaccggg
     7261 ctgggctgcc gcgtgctctg ctatgacctg tacggccgtg gcttctccga ccggccccgc
     7321 gtgacgtaca ccctggacct gttcgtggac cagctccgcg agctgctcgg cgcgctggcc
     7381 gtccgcgtgc cggtgcgtct ggcaggcctg tccatgggcg gggagatcgc ggcggcattc
     7441 accgcgcgga atcccgagct ggtggatcgg ctctgtctca tcgatccgca ggccatcccg
     7501 atcacgcgga agacgctctt ccccctgggt gtgcccggcg tcggcgagta cctgatgacc
     7561 gtgtacgtca tccccttcgt cctgtcctcc ccggtcggag acttctacca gccgcaatcc
     7621 ctgccgggat ggggggatcg ataccggccg cagactacgt accgggggtt ccgccgggcg
     7681 cttctctcca ccctgcggca cttccccaaa gactacatgg ccgcctatcg ccgggtgggc
     7741 gagctcggca agcccgtgct cctgctctgg ggagagaagg acacgtcgat ccccatctcc
     7801 aacgcggcgg ggctccgcgc cgccataccc caggtggagt tccaccccat cgcgttggcc
     7861 gggcacctgt ccaactacga gcgtccggat ctggtcaatc ctctcctgac cgcgttcctc
     7921 gttcgtcgct gagcgacgtt ctgggtatgt tgagggagcg cgggcagcgc tccgggatgg
     7981 cgagtgcccg tcaaggagga agcgatggac tatcgaagac ttgggaccgc gggcctgaag
     8041 ctgagcgtgc tgtccctggg ctcctgggtg acctacgggc ggcaggtgga cgtggatgcc
     8101 gccgcggcct gcatgaaggc cggctacgag gaaggcgtga acttcttcga taacgccgag
     8161 acctacgagg ccggcaagtc cgaagaggtg atgggcgccg cgcttgccaa gctgggctgg
     8221 cgccgcgcca gctacgtggt ctcgacaaag atctacttcg ggatgcatcg gggcgtgaac
     8281 gaggagcgca ccctcaaccg caagaagctg cgggagggga tcgacggcgc gctgaagcgc
     8341 ttcggcctgg actacatcga cctggtgttc tgccatcgcc cggactccga gaccccggtg
     8401 gaggagacgg tgtggtcgat gcacatgatg gtggagtccg gcaaagcgct gtactgggga
     8461 acctccgagt ggagcgccga cgagctcatg gaggcgtggg ctatcgcgga gaagcatcac
     8521 ctccacaagc cccagatgga ccagccccag tacaacctct tcgtgcggcg tcgggtggag
     8581 agggaatacc agcggctgta cgaccgcatc ggcctgggcc tcaccatctg gagcccgctg
     8641 gctgccgggc tcctcaccgg caagtacaac gccggcatcc cccagggcgc ccggatgggg
     8701 ctcccgggct acgagtggct caagagcgat ccgctgcagc gcggcgtgcc ggccgggacc
     8761 gcggggacgc cctcctttgc cgcgctggtg gaggcggcga agaggctcga gcccgtcgcg
     8821 aaggagctcg gggcaaccca ggcgcagctc gccctggcgt ggtgcgccca caatccgcac
     8881 gtaagctccg tgatcaccgg cgccagccgg cccgagcagg tgaaggagaa catgaaggcc
     8941 attgccctcc tgcccaggct caccccggag gtcgtgagca ggatcgagaa ggccattggc
     9001 ggggccagcg acgtctcctg acgaccgcct cgggaagccg ccggccggcg gacgctagga
     9061 tgcgcccgcg gtggcggcgg agagcttcgc ggccaggtct ggcgcgatct gctcgtgctt
     9121 gaagaacacg aacgcctcgt cccatccctg ttcggcgatc cgctgggacc aggcggtgag
     9181 ctcttcgggc tggtaggaag tcttgcgcag acgcaggtat ccccacggcg ccgtcttcac
     9241 gagggccggc tcggggtcct catccgtctg gctgatgacc agggcgaccg agtgcttctc
     9301 cagcagggcg aagacggcgg gatctcgcca ggagtcgttg cggacttcga gggcggcacg
     9361 catccccttg ggaaggaggt tgaggaaggc gaacaggcga tcgtcgtctc gggccagatt
     9421 cggcgggatc tggaccagga gcgcgcccag cttgggtccc agggctgccg ccgtctcgac
     9481 gaggtaggcg gtctcctcat cgacgttgcg aagccgcttg aagtgggtga tcttctggga
     9541 gactttgagg acgaatcgaa acccttccgg gacctgggat gcccagctcg agagcagttt
     9601 ggtggtcggc aaccggtaga aggtattgtt gatctccacc gtgccaagcc gctccgcgta
     9661 gaaggagagc atcttcgccg ccgcgagcgt ttccgggtag aaggtcccct tccactcctt
     9721 gtagctgtag ccgctggtcc cgatccgcag ctccataccc ggaagatact ccctgccctt
     9781 gacgccgccc accaccacgc gcatcgtgga ccgatgaagg ctttcgtgat gaccgacctg
     9841 gaaggcgtgg cgggcgttgt ctccttcgag gagcagaccc gacccgacac ccgctacggg
     9901 gagcaggcca agcgcctcct caccgcggag gtcaactcgt gcgtccaggg cctgcgggag
     9961 gccggcgtca cggacgtggt ggtcctggac ggccacggcg acggcgggat cgtgtacgag
    10021 gagatccatc cggaggcccg cctgatccat ggcaagccgc tcgcggagga gtggcgcaac
    10081 cagatggaag ggatggatgt ggcggtgatc gtgggtcagc acgccatggc cggcgttccg
    10141 gacgggaacc tcaaccacac ccaggactcc cggtccatca cgtacttcac gctgaacgga
    10201 cgactcatcg gagagacggc tcagttcgct ctctttgccg ggtcctacgg cgtgccgctg
    10261 atcttcctct ccggagacga agcggcctgt cgggaggcgc aggagctcat cccgggtgtc
    10321 acgacggcgg ccgtgaagaa gggcctgggc cgaaactcgg cggtctccgt gagcgcgccg
    10381 aggtcgcggg agctgatccg cgcgggcgcc gccgcggcgg ttgcccgaca gcgcaccagc
    10441 cccctgaagc cgctggtatg gccgggtccc tacgtgctgg agaagcgctt cttcggaacc
    10501 aaccacctgg agcagtaccg gtacctcgcg acggtgaacc ccaacatcac gatggtggat
    10561 cctctcaccg tacggattcg gggcgacgac atccgtgcgc tgatctacgc gtgacgatca
    10621 tgaccagccg gcagcgaacg agcgcggccg
//
LOCUS       DASRZS010000406         6146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_612613, whole genome shotgun sequence.
ACCESSION   DASRZS010000406 DASRZS010000000
VERSION     DASRZS010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612613"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3369
                     /gene="rpoC"
                     /locus_tag="VFH83_09905"
     CDS             <1..3369
                     /gene="rpoC"
                     /locus_tag="VFH83_09905"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET6486722.1"
                     /translation="FDNGRRGRVLRGANNRPLKSLSDTLKGKQGRFRQNLLGKRVDYS
                     GRSVIVVGPDLKLHQCGLPSKMALELFKPFIMRKLVQKDVVYNIKKAKTLVEQESAEV
                     WAVLEEVVQEHPVMLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCHAFNADFDGDQM
                     AVHVPLTPEAQTECWTLLLASKNLLNPASGNPIAVPTQDMVLGINYLTRERAGALGEG
                     KHFSSYDECILAVDAKGVDYQATVKVKVGDQWVDTTPGRVILNDNVPDDLGFLNYAMG
                     EKELLNLIARCYKEKGPAVTVKMLDAVKELGFKYATRFGATIGMDDIVIPEAKKSLIK
                     EANSKVDEIQRQYLQGHITNEERYNRVIEVWSSTNELITNELMDELKRDHEGFNPVYM
                     MAHSGARGSRSQIRQLAGMRGLMAKPSGDIIELPIRSNFKEGLSVIEFFISTNGARKG
                     LADTALKTADAGYLTRRLVDIAQDVVISEEDCGTINGIDRRAIKDGEDIVEHLRDRIS
                     GRFSLEKVKHPITGEVIVDQNEEITDEISVSIEEAGIEAVRIRSVLTCDSKYGVCARC
                     YGRNLATYKPVEIGEAVGIIAAQSIGQPGTQLTMRTFHIGGAATKISEENRIYLKYPV
                     FVEKIQGSMVKIDNGNMLFTRKGSIQVHRILESHELQKGDRVLVKDGQRLLKHEPEPL
                     IQRKGETISSKEICYVKLLPDRLLLIAQPQLIEIRNGAEVIAKEGMAVPGEETIAFFD
                     PFSEPIITEATGTIRFHDIVLGTTLKEEVNEDTGKIEKKITEYTLETLQPRITVEDDK
                     GNELAGYFLPGNAYLNVDDGDTVKAGRIIAKLLRESVKTSDITGGLPRVGELFEARKP
                     KNPSVLAKVSGRVSFKGIVKGKRVIVVTDPFNRELKHLVPMGKHLLVREADEVEAGDM
                     LCDGPKDPHDILQILGETELHSYLLNEIQEVYRLQGVRINDKHIGVIIRQMMKKVEIV
                     DVGDTPFIYGQQVDRYKFHEENRKTLREGGQPAVARPILLGITRVSLNIDSWLSAASF
                     QETTRVLTNASISGSVDHLRGLKENVIIGHLIPAGTGMKKYREIKLYDENMEDLDISI
                     SRIIEEKRKERELKAMLEAEKVALPNAESVLAPD"
     gene            3680..4054
                     /gene="rpsL"
                     /locus_tag="VFH83_09910"
     CDS             3680..4054
                     /gene="rpsL"
                     /locus_tag="VFH83_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012538144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET6486723.1"
                     /translation="MPTINQLVRIGRKAARKHTKAPALQNCPQKRGVCTRVMTVTPKK
                     PNSALRKVARVRLSNGIDVTAYIPGIGHNLQEHSVVLIRGGRVKDLPGVRYHIIRGAK
                     DTLGVEDRKKARSKYGAKKPKA"
     gene            4069..4542
                     /gene="rpsG"
                     /locus_tag="VFH83_09915"
     CDS             4069..4542
                     /gene="rpsG"
                     /locus_tag="VFH83_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET6486724.1"
                     /translation="MPRGQLIRKKRIVLDPKFKNPVVGKFVTRMMEQGKRSISFSTMY
                     GALEIIGQKVQGKEPVEVFQKALENVKPVVEVKSRRVGGATLQVPVEIREERRDALAM
                     RWVIGFSRSRHGRSMSEKLAAELIDAFNNTGSAIKKKEDTHKMAEANKAFAHYRW"
     gene            4558..>6146
                     /gene="fusA"
                     /locus_tag="VFH83_09920"
     CDS             4558..>6146
                     /gene="fusA"
                     /locus_tag="VFH83_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET6486725.1"
                     /translation="MESRFPLSSIRNIGIMAHIDAGKTTTTERILYYTGRTHKLGEVH
                     DGEAKMDWMDLEKERGITITAAATSCQWRGVQINIIDTPGHVDFTVEVERSLRVLDGA
                     IGVFSAVEGVEPQSETVWKQANRYQVPRVAFINKMDRVGARFEAVVQELHTKLGANAV
                     ALQVPIGREADFVGVVDLVRMRALVWDEDVLGAQFREMDIPADLQETARAARTTLLEK
                     LAETDDAIMAAYLDGGEIGEEKLKGALRAATVQGRIFPLVCGTAFKNKGIQPLLDAVV
                     DYLPSPGDIPPATGMVPSTGDYIQRQPKDDEPFAGLAFKIMTDPFVGKLTFFRVYSGT
                     LGAGSYVYNANSDRRERITRILRMHANEREEIKAVYSGDIAAAVGLKKTTTGDTLCDQ
                     EHPVILESMVFPEPVVSVAIEPKTRDDEEKLSSALHKLSEEDPTFKVRFDPETGQTII
                     HGMGELHLEVLVERMSREFNVAANVGRPEVAYRETIRGEAETEGKFIKQTGGHGAYGH
                     VVMRFESLPPGTGFVFENKVIG"
BASE COUNT         1297 a         1845 c         2049 g          955 t
ORIGIN      
        1 ttcgacaacg gccgtcgcgg ccgcgtcctc cgcggggcca acaaccgccc cctgaagtcc
       61 ctctccgaca ccctgaaggg gaagcaggga cgcttccgac agaacctcct gggcaagcgc
      121 gtggactact cgggccggtc cgtcatcgtg gtcggccccg atctgaagct gcaccagtgc
      181 gggctgccgt ccaagatggc gctggagctg ttcaagccct tcatcatgcg caagctcgtg
      241 caaaaagacg tcgtctacaa catcaagaag gcaaagacgc tggtcgagca ggagagcgcg
      301 gaggtgtggg cggtcctgga ggaagtggtc caggagcatc cggtcatgct gaaccgcgcg
      361 ccaacgctgc accgcctggg tatccaggcc tttgagccca tcctggtgga aggcaaggcc
      421 atcaagctgc accctctggt ctgccacgcg ttcaacgcgg acttcgacgg ggaccagatg
      481 gccgtgcacg tgcccctgac ccccgaggcg cagacggagt gctggacgct cctgctggcc
      541 tccaagaacc tgctcaaccc ggccagcggg aaccccatcg ccgttccgac gcaggacatg
      601 gtgctgggga tcaactacct gacccgggag cgcgcgggtg ccttgggaga gggcaagcac
      661 ttctcgagct acgatgagtg catcctggcc gtggacgcca agggcgtcga ttatcaggct
      721 acggtcaagg tcaaggtggg cgaccagtgg gtggacacca cgcccggacg ggtcatcttg
      781 aacgacaacg tccccgatga cctgggcttt ctgaactacg ccatgggtga gaaggagctt
      841 ctgaacctga tcgcccggtg ctacaaggag aagggcccgg cggtcaccgt gaagatgctg
      901 gacgcggtga aggagctggg tttcaaatac gccacccggt tcggagccac catcggaatg
      961 gacgatatcg tcatcccgga ggccaagaag agcctcatca aggaggcgaa ctccaaggtg
     1021 gacgagatcc agcgccagta cctccagggc cacatcacca acgaggagcg gtacaaccgc
     1081 gtcatcgagg tgtggagctc caccaacgag ctcatcacca acgagctcat ggacgagctg
     1141 aagcgtgacc acgagggctt caacccggtg tacatgatgg cgcactccgg tgcccgaggg
     1201 tcccgcagcc agatccgcca gctcgcgggc atgcgcggcc tgatggccaa gccgtccggg
     1261 gacatcatcg agctccccat ccggtcgaac ttcaaggaag gcctgtcggt catcgagttc
     1321 ttcatctcga ccaacggcgc ccgaaaaggt ctggcggata cggccctcaa gaccgccgac
     1381 gccggctacc tcacgcggcg gctggtggac attgcccagg acgtcgtcat ctccgaggag
     1441 gactgcggga cgatcaacgg gatcgaccgc cgcgccatca aggacggtga ggatatcgtc
     1501 gagcacctga gagaccggat ctcgggacgc ttctccctgg agaaggtgaa gcacccgatc
     1561 accggcgagg tgatcgtgga tcagaacgag gagatcacgg acgagatctc cgtctccatc
     1621 gaggaggcgg gcatcgaggc cgtgcggatc cgctccgtgc tgacctgtga ctccaagtac
     1681 ggcgtctgcg cccgctgcta cggccgcaac ctcgccacct acaagccggt ggagatcggg
     1741 gaggcggtgg gtatcatcgc ggcccagtcc atcggccagc ccggcacgca gctcaccatg
     1801 aggaccttcc acatcggtgg ggcggccacc aagatcagcg aggagaaccg gatctacctc
     1861 aagtacccgg tctttgtcga gaagatccag ggcagcatgg tgaagatcga caacggcaac
     1921 atgctcttca cccgcaaggg gtccatccag gtgcaccgga tcctggagag ccacgagctt
     1981 cagaaggggg accgcgtcct ggtgaaggac gggcagcgcc tgctcaagca cgagccggag
     2041 cccctcatcc agcgcaaggg agagacgatc tcctccaagg agatctgcta cgtgaagctc
     2101 ctgccggacc ggctcctgct catcgcgcag cctcagctca tcgagatccg aaacggagcc
     2161 gaggtcatcg ccaaggaggg catggccgtc cccggggagg agaccatcgc cttcttcgac
     2221 ccgttcagcg agccgatcat cacggaagcg acggggacca tccggttcca cgacatcgtc
     2281 ctggggacca ccctcaagga ggaggtcaac gaggataccg gcaagatcga gaagaagatc
     2341 accgagtaca ccctggagac cctgcagccg cggatcaccg tggaggacga caagggcaac
     2401 gagctggccg ggtatttcct cccggggaac gcctacctca acgtggacga cggagacacc
     2461 gtgaaggcgg ggcggatcat cgccaagctg ctccgcgaga gcgtgaagac cagcgacatc
     2521 accggcggtc tgccgcgcgt cggggagctc ttcgaggcgc gcaaacccaa gaatccttcg
     2581 gtcctggcca aggtgagcgg gcgggtttcc ttcaagggca tcgtcaaggg caagcgggtc
     2641 atcgtggtca cggacccgtt caaccgcgag ctcaagcatc tggtgcccat gggcaagcac
     2701 ctcctggtcc gtgaggccga cgaggtggag gccggggaca tgctctgcga cggccccaag
     2761 gacccgcatg acatcctcca gatcctcggg gagacggagc tgcacagcta cctgctcaac
     2821 gagatccagg aggtctaccg cctgcagggt gttcggatca acgacaagca catcggcgtc
     2881 atcatccgcc agatgatgaa gaaggtggag atcgtcgacg tcggcgacac cccgttcatc
     2941 tacggccagc aggtggaccg ctacaagttc cacgaggaga accgaaagac gctgcgcgaa
     3001 ggaggccagc cggccgttgc tcggcccatc ctcctgggca tcacgcgggt ctccctgaac
     3061 atcgactcct ggctgtcggc tgcctccttc caggagacca cgcgcgtgct caccaacgcc
     3121 tccatctcgg ggagcgtgga tcatctgcgc ggcctgaagg agaacgtgat catcggacac
     3181 ctgatccccg ccggtaccgg catgaagaag taccgcgaga tcaagctgta cgacgagaac
     3241 atggaagacc tggacatctc catcagccgc atcatcgagg agaagcgcaa ggagcgggag
     3301 ctcaaggcca tgctggaggc cgagaaggtg gccctgccca atgcggagag cgtcctggcg
     3361 ccggactagg gttttccggg acggcggagc ggccgctccg ccgtccgctt ccatccctgg
     3421 acagtctcgc cgaaaagcga taggctgggc gtgttctggg aggggatccg gtttcgaatc
     3481 ggagcgccgg atgagtcatc cggcgtagat cgggttgggt ttcttgggat aggcctcgtg
     3541 aacccgctct gaaggtatgg agggggttga cttttttttc gtgagtcagt accgttatac
     3601 accgcgactt tggggactct tgcgctttcg gaaagcatcg cgagacaaac gggacggaga
     3661 aaaggggagc aggaaaggaa tgccgacgat caaccagttg gtgcggatag gacggaaggc
     3721 cgctcgcaag cacacgaagg ccccggctct gcagaactgc ccgcagaaga ggggagtgtg
     3781 cacgcgtgtc atgacggtca cgcccaagaa gcccaactcg gccctccgaa aggtggcgcg
     3841 cgtccggctc tccaacggta ttgacgtcac ggcctacatt cccggcatcg gccataacct
     3901 gcaggagcac tcggtggtac tcatccgcgg cggcagggtc aaggacctcc cgggcgtccg
     3961 ctatcacatc atccgcgggg caaaggacac cctgggcgtg gaagaccgca agaaggcccg
     4021 gtccaagtac ggcgccaaga agcccaaggc gtaagggaga gaagcgcgat gccgcgtgga
     4081 cagctgattc gaaagaagag aatcgtcctc gaccccaagt tcaagaaccc ggtcgtcggc
     4141 aagttcgtga cccggatgat ggagcagggg aagcggagca tttcattttc gacgatgtac
     4201 ggcgccttgg agatcatcgg ccagaaggtg cagggtaagg agccggtgga ggtgttccag
     4261 aaggcgctgg agaacgtgaa gccggtcgtc gaggtcaagt cccgacgcgt aggcggcgcc
     4321 accctgcagg tgccggtgga gatccgtgag gagcggcgcg atgcccttgc catgcgctgg
     4381 gtcatcggct tctcccgcag caggcacggc cggtccatgt cggagaaact ggcggccgag
     4441 ttgatcgatg ctttcaataa tacgggatcc gcgatcaaga agaaggaaga cacgcacaag
     4501 atggcggagg cgaacaaggc gtttgcccac taccgctggt aaagcggcgc cgtccgtatg
     4561 gagtcgcggt tccccctgtc gtcgatccgc aatattggga tcatggccca tattgacgcc
     4621 ggtaagacga cgacgacaga gaggattctt tactataccg gcaggacaca caagctgggc
     4681 gaggtccatg acggcgaggc caagatggac tggatggacc tcgagaagga gcgcggcatc
     4741 accataacgg cggccgcgac ctcctgccag tggagaggcg tgcagatcaa catcatcgac
     4801 acgccggggc acgtggactt caccgtcgag gtggaacgat ctttgagagt gttggacgga
     4861 gcgatcgggg tgttttccgc cgtggaaggc gtagaacccc agagtgagac cgtctggaag
     4921 caggcaaacc gttaccaggt cccccgtgtg gcgttcatca acaagatgga ccgagtcggg
     4981 gcgcgcttcg aggccgtggt ccaggagctt cataccaagc tcggggccaa cgcggtggcc
     5041 ctccaggtcc ccatcggacg ggaggcggac ttcgtcggcg tcgtagacct cgtgcggatg
     5101 cgggcgctcg tgtgggacga agacgtgctg ggcgcccagt tccgcgagat ggacatcccc
     5161 gccgatctcc aggagacggc ccgcgcggcg cggaccacgc tgctggagaa gctggcggag
     5221 acggatgacg ccatcatggc cgcgtacctg gacggaggag agatcggtga ggagaagctg
     5281 aagggcgcgt tgcgcgctgc aacggtccag gggcggatct tcccgctggt ctgcgggacc
     5341 gcgttcaaga acaagggcat ccagcccctg ttggacgccg tagtggacta cctgccgtct
     5401 cccggcgaca tcccgccggc caccggcatg gtgccgagca cgggagacta catccagcgg
     5461 cagcccaagg acgatgagcc gttcgcaggg ctggccttca agatcatgac cgatccgttc
     5521 gttgggaagc tgacgttctt tcgggtctac tcggggacgc tgggcgccgg gtcgtacgtg
     5581 tacaacgcga actcggaccg ccgggagcgg atcacccgga tcctgaggat gcacgccaac
     5641 gagcgggagg agatcaaggc ggtctactcg ggggatatcg ccgccgcggt gggactgaag
     5701 aagaccacca cgggagacac gctgtgcgac caggagcacc cggtgatcct ggaatccatg
     5761 gtgtttccgg aaccggtcgt gtccgtcgcc atcgagccga agaccaggga tgacgaggag
     5821 aagctctcct cggctctgca caagctctcg gaggaggacc ccaccttcaa ggtgcggttc
     5881 gatcctgaga cggggcagac gatcatccac gggatggggg agctgcacct ggaagtcctg
     5941 gtggagcgga tgtcccggga gttcaacgtg gcggccaacg tcgggagacc cgaggttgcc
     6001 taccgcgaaa cgatccgggg agaggcggag acggagggga agttcatcaa gcagaccggc
     6061 gggcacgggg cgtacggcca cgtggtaatg cgcttcgagt cgctgccccc gggaactggt
     6121 ttcgtctttg aaaacaaggt catcgg
//
LOCUS       DASRZS010000407         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3683423, whole genome shotgun sequence.
ACCESSION   DASRZS010000407 DASRZS010000000
VERSION     DASRZS010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3683423"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFH83_09925"
     CDS             complement(<1..264)
                     /locus_tag="VFH83_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HET6486726.1"
                     /translation="MNPNSPAEISTLLSVLGLTLKKRWGQNFLVNPGARERLVSLLQP
                     SARELVWEIGAGLGSMTELLLPKVDRLVAFEVDWGLCRYLEDRF"
     gene            complement(261..1769)
                     /locus_tag="VFH83_09930"
     CDS             complement(261..1769)
                     /locus_tag="VFH83_09930"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET6486727.1"
                     /translation="MLRRHLTPAVILFLILLAGCLARGALGGRWWLLLAAAAPLAATA
                     RVLTPLASETLRKAGLVTGICCAGLVCGAALLAAMEDRTRAAYLPVPPAQVTSFTGSL
                     VADSTLTAKGFTALRVRLSRATAAAHGMQGAARGEALLLVSGSHLFTLGQVISVTSPL
                     GAVREPGEQAWISYPLRGEIHVQGFTSRVWELRAQARGWMHGAFERAGYPASALMEAL
                     ILGSREEIPSDLQDGFKRTGSLHILALSGLHVTIIWGLLWGLFSFVRRTGARTAVACL
                     LLVLYQVIAGFMPSLLRATVMVLVASVSLPLDRDREPLNLLALSGILLLLMDPFQAFS
                     LSFQLSFAAMAGILLIAPVIQGPVSRFLPPFLSAPIAMSIAAQVATFPLIVSQFGVWY
                     PSGIVAGLLLVPLTTALLWGGLAWLVLVLIPVPGLHAVCVRLFGVVYAATEGVAGWSS
                     RLPGLTVSAATAAAVVAASALVAAAICLWVPIPAIRPRKRSRARSLDPACQP"
     gene            complement(1834..3021)
                     /locus_tag="VFH83_09935"
     CDS             complement(1834..3021)
                     /locus_tag="VFH83_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486728.1"
                     /translation="MNRAVVILLAFVVCVASVEAQDAQKVVDTYKRNFAIASLDVKIQ
                     ILQDATTNPNAAAMGPLYEQALEFALDNASLIDTDVRFSQLAAIAAKQVGAINYKDAA
                     LSLWRLFPAGANTEALTAAAGALAVVAAGNVDLITNLNRYVDSMNTLYTTGKMPDPAV
                     LSACIQALGALGDPSSFPTLFSAVNLGYSDQITAMARAALLSIKGDLGQMLQEVMRTR
                     PMEEKKLALQMALDNSRLTDDQKGAVAEYALDVGLHTSVPDAPGKATLRDIRFLAASA
                     VAARKRAHASGLMVENLDMTISEYDKNLVDRGRLLDSIAELGAMGTHEAAARLTQYLV
                     LLNSYVEKGKTYDESIMLTLIGSLGTLGDKVAFDDLMYTQYLQYSATVKKAARASLDK
                     LQW"
     gene            complement(3126..>3385)
                     /locus_tag="VFH83_09940"
     CDS             complement(3126..>3385)
                     /locus_tag="VFH83_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486729.1"
                     /translation="SGVVKGVNFDQQKNRSVVHVQAAAPSVLVRNRIHMYVYNLFGER
                     EAGPSPRNGDRAARPEEGIVIRAGSPEDGPSVAPKASAEAG"
BASE COUNT          616 a         1114 c         1145 g          510 t
ORIGIN      
        1 gaaccggtcc tccagatacc gacaaagccc ccagtccacc tcgaacgcga ccaggcggtc
       61 taccttgggc agaaggagct ccgtcatcga gcccaggccg gcgccgatct cccatacgag
      121 ctcccgggca ctcggttgaa gcagggaaac cagtctttcc cgtgcccccg gattgacaag
      181 gaagttctgg ccccagcgct tcttcagggt cagccccaac acggagagga gcgtcgagat
      241 ctccgccggg gagttggggt tcacggctgg catgccggat cgagcgatcg agcccgtgac
      301 ctcttgcggg gccggatcgc gggtatgggg acccacaggc agatcgctgc cgcgaccagc
      361 gcagacgcag cgaccaccgc ggcagccgtg gcggccgaga cggtcagccc cggcagccgg
      421 gaagaccagc cggccacgcc ctccgtggcc gcatacacca cgccgaaaag acggacgcag
      481 acagcgtgga gacccggcac ggggatcaga acgaggacca gccaggcaag ccctccccag
      541 agcaaggcgg tggtaagggg aacgagcagc agtcccgcga cgattccgga cggataccag
      601 acgccgaact gggagacgat caatgggaaa gtggccacct gggccgcgat gctcatggcg
      661 atgggcgcgg agagaaaggg cggaaggaat cgggacacgg gaccctggat caccggcgca
      721 atcagaagga tccccgccat ggccgcgaag gaaagctgaa acgagaggga gaacgcctga
      781 aaggggtcca tgaggagcag gaggatcccg gaaagggcca gcaggttcag tggctcgcgg
      841 tctctatcca gcggcaggga aacggaggct accagcacca tcacggtggc gcgaaggagg
      901 gagggcatga atccggcgat gacctggtag aggaccagaa gcaagcatgc aacggccgtc
      961 cgagcgccgg tccgccggac gaacgagaag agtccccaca gcaggcccca gatgatcgtg
     1021 acatggagcc ccgaaagcgc caggatatga aggctgccgg tcctcttgaa gccatcctgc
     1081 agatcggacg ggatctcctc ccggctcccc aggatcaggg cctccatgag cgcggacgcc
     1141 ggatatccgg ctcgctcgaa cgctccgtgc atccagcctc gggcctgggc cctcagctcc
     1201 cacacccggc tggtgaaccc ctggacgtgg atctctcctc gaagaggata ggatatccac
     1261 gcctgttctc ccggctcccg caccgcccca aggggcgaag tcaccgagat cacctggccg
     1321 agggtgaaaa gatgactgcc cgacacgaga agcaacgctt cccctcgcgc cgcgccttgc
     1381 atcccgtgag ccgccgcggt agcgcgggac aggcgtaccc tcagggccgt gaagcccttg
     1441 gcggtcaggg tgctgtccgc caccaggctc ccggtgaacg aggtgacctg cgcggggggc
     1501 accggaaggt acgccgcccg cgtgcgatcc tccattgcgg ccaggagcgc cgcgccgcag
     1561 accagtcccg cgcaacagat tcccgtcacg agtccggcct tccgaagcgt ctcggaggcc
     1621 agcggcgtga gcacgcgcgc ggtggccgcc aggggtgccg cggcggcgag gagcagccac
     1681 cacctcccgc ccagcgcccc gcgcgccagg cagccggcca ggaggatgag aaagaggatc
     1741 accgctggcg tcaggtgccg acgcagcatg tccggtgggg gagctcagct cacggacgga
     1801 cggttgagaa gggttgccgt gggaagcggg tggctaccac tggagcttgt cgagtgacgc
     1861 ccgtgccgct ttcttcaccg tggcgctgta ctgcagatac tgggtataca tcaggtcatc
     1921 gaacgcgacc ttgtccccaa gggtgcccag gcttccgatc agcgtcagca tgatcgactc
     1981 gtcgtaggtc ttgcccttct ccacgtagga gttgaggagc acgaggtatt gggtgagccg
     2041 ggccgccgcc tcatgagttc ccatggcccc cagctccgcg atggaatcca ggagccggcc
     2101 gcgatccacc aggtttttgt cgtactccga gatggtcatg tccagattct ctaccatgag
     2161 gccggaggcg tgcgcccgct ttcgggcggc cacggcgctt gccgccagga aacggatgtc
     2221 cctcagggtg gccttgccgg gcgcgtccgg cacgctggtg tgcagtccga cgtccagcgc
     2281 gtactccgcg acggcgcctt tctggtcgtc ggtgagccgg ctgttgtcga gcgccatctg
     2341 gagggcgagc ttcttctctt ccatgggccg ggtgcgcatg acctcctgga gcatctggcc
     2401 gaggtctccc ttgatggata gcagagcggc gcgggccatg gcggtgatct gatccgagta
     2461 gcccaggttg accgcgctga agagggtggg gaacgaggac gggtccccga gcgcgcccaa
     2521 cgcctggatg cacgcggaca ggaccgcggg gtccggcatc ttcccggtgg tgtagagcgt
     2581 gttcatggaa tccacgtagc ggttcaggtt ggtgatgagg tccacgttac cggcggcgac
     2641 cacggccagc gctcccgccg ccgcggtcaa ggcctccgtg ttggcccccg ccggaaacag
     2701 ccgccacaac gagagcgcgg cgtccttgta gttgatggcc ccgacctgct tcgcggcgat
     2761 ggccgcgagc tggctgaacc ggacgtccgt gtcgatgagg ctggcgttgt ccagcgcgaa
     2821 ctccagcgcc tgttcgtaca agggccccat ggccgcggcg ttcggattgg tggtggcgtc
     2881 ctggaggatc tggatcttga cgtcgaggct tgcgatggcg aagtttcgct tgtaggtgtc
     2941 caccaccttc tgggcgtcct gagcctcgac ggaggccacg cataccacga aagccaacag
     3001 gattacgacg gcacgattca tatgaaggcc tccacctccc ccgcggcaga cttccattcc
     3061 ggcgcttccg gcgcgccggc taccggcagc cagaccggcc cttccctggg gcccgcgccc
     3121 cggcttcaac cggcctccgc ggacgccttt ggcgccacgg agggcccatc ctcgggcgaa
     3181 cccgccctga tcacgatccc ttcctccggg cgcgcggccc gatcaccgtt gcgcggggaa
     3241 ggtccggcct ctcgttctcc gaacaggttg tagacgtaca tgtggatccg gttgcggacc
     3301 aggaccgagg gagccgcggc ctgcacgtga accacggacc ggttcttctg ttgatcgaag
     3361 ttgacaccct tcacgacgcc ggaca
//
LOCUS       DASRZS010000408         4058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4072123, whole genome shotgun sequence.
ACCESSION   DASRZS010000408 DASRZS010000000
VERSION     DASRZS010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4072123"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3080
                     /locus_tag="VFH83_09945"
     CDS             <1..3080
                     /locus_tag="VFH83_09945"
                     /inference="COORDINATES: protein motif:HMM:NF021504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HET6486730.1"
                     /translation="ILEIGGQDAKYTYLVNGVPCDYAMNEACSAGTGSFLEEAARESL
                     DVDYRDIEGIAMTAQAPPNFNDQCAAFIASDIKSAAHERVSRADIVAGLVYSICMNYT
                     NRVMGSRRIGNRVLMQGGVCYNRAVPLAMAALLQRPIVVPPDPGLMGAFGVALEIVHR
                     MSLGLLARCRYDLRRLQDREVTHGRTFRCAGGSEKCDRGCTVNTLRIDGKTFPFGGAC
                     NRYYELRRRAGAAVGRNLVQARQDLLFASVPSSPPSADDGPTIGINRSFYMHTLYPLF
                     SSFFSGLGLQVISSNGIDPAGASSVSSSFCFPTEIAHGAFANLLSLGPDYLFLPQIQE
                     QFVENSGTTEPESHCSCVIGQAEPYYLRSVFRDIRSRIVSPVLNFSTGWSALVPQMAS
                     VATQVGATMAEGLAAAELAVEHQLEFGRARKEIGDRLLGELDRPDAPIAVVLFGRAYN
                     AFPGEANLGIPQKFASRGVSVVPFDCLPTKAEESIDGMSWASGHEIIKAARFVKGHPR
                     LFGCYITNFSCGPDSFLVGYFRDIMKTKPSLTLELDSHSADAGINTRVEAFLDIIERS
                     VRIGVRDSHREPFVPARVEPGKRGFQFVCSDGKRVPLSVAEILIPSMGDITSRLGASA
                     LRGMGFSARHAPIPDFPVLMTGRKNTSCKECLPMILTTGTLLTELKDRPGSSPLVYFM
                     PAQGGNCRFSQYTVFLQKLIAEKRLANVALWSLNSNNGYGGLSVPALLSILRSIIVSD
                     CLDDIKNAVLALARDREKGMTVFEEALSRISRCMERGSRGLMRTLRGVARMLSSIELT
                     GPLHQARTVLVAGEIYIRKDNFSSDVLVERLARRGIVCRRAPITEWLAYVDYYAQHLI
                     RSGMSLSERVYLVGKKLIQARMERRIKKALAASGLCENEPVDIPALIRVGRRFVREEL
                     TGEIILVIGAFFREMIDHIHGLVSIGPFACLPSRVIESILSVESNVRNNERLKSLETY
                     DAIRRFSSLPFLAVECDGNPFPPIIETRIEAFALQVERVHAQARESQDGRHRS"
     gene            complement(3161..3664)
                     /locus_tag="VFH83_09950"
     CDS             complement(3161..3664)
                     /locus_tag="VFH83_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018887903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6486731.1"
                     /translation="MYTSISEFVEDWTRESGNSLKVERMLTDASLSQKADAESNTLGK
                     IAWHMVVMIGLTATGAGLTVDAPPRGTEPPATAAAIADAYERAARSMAEQASARLKDD
                     QLGTEIPLFGRTIKLTVALQGLIRHQIHHRGQMTILMRSAGLAVPGVYGPSREEAAAL
                     RAKQAGR"
     gene            complement(3703..>4058)
                     /locus_tag="VFH83_09955"
     CDS             complement(3703..>4058)
                     /locus_tag="VFH83_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HET6486732.1"
                     /translation="FPTICGTGTEDYFGGAYNWDVDGRYVPYSTPFMGMHQVIKPDGL
                     YASQHRHAMYRWHIPDPIRFEKDIKVTIQGLGWRNGGRYLPMQDDIASVACWYQTLPT
                     APFPALPDRDYLEII"
BASE COUNT          675 a         1327 c         1328 g          728 t
ORIGIN      
        1 ccatcctgga gatcggcggg caggacgcca aatacaccta cctggtgaac ggcgtcccct
       61 gcgattacgc catgaacgag gcctgttccg ccgggaccgg gtcctttctc gaagaggccg
      121 cgcgggaaag cctggatgtc gactatcggg acatcgaggg catcgccatg accgcgcagg
      181 cgcctcccaa cttcaacgac cagtgcgccg cgttcatcgc gagcgacatc aagagcgcgg
      241 cccacgaacg ggtgagccgt gcggacatcg tggcgggtct ggtctattcc atctgcatga
      301 actacaccaa ccgggtcatg gggtcccgca ggatcgggaa ccgggtgctc atgcaggggg
      361 gcgtatgcta caaccgcgcg gtgccgctgg ccatggcggc gctcctgcag cggcccatcg
      421 tggttcctcc ggatccgggg ctcatgggag cgttcggtgt ggcgctggag atcgtccatc
      481 ggatgagcct gggcctcctg gccaggtgcc gatacgatct acgccgtctc caggaccgag
      541 aggtgacgca cgggaggacg ttccgatgcg ccggaggaag cgagaagtgc gaccggggct
      601 gcaccgtgaa cacgttgcgg atcgacggga agaccttccc gttcggcggc gcctgtaacc
      661 ggtactacga gctgcgtcgc cgggctggcg cggccgtcgg acggaatctc gtgcaggcgc
      721 ggcaggacct cctctttgcc tcggtcccgt ccagtccgcc ttcggcagac gacgggccga
      781 ccatcgggat caaccgaagc ttctacatgc acacgctgta cccgctcttt tcgagcttct
      841 tcagcggcct tgggctccag gtgatcagca gcaacggcat cgatcccgcc ggcgcctctt
      901 ccgtcagctc ttcgttctgc ttccccacgg agatcgcgca cggggcattc gccaacctcc
      961 tgagccttgg cccggattac ctctttctgc cccagatcca ggagcagttc gtggagaact
     1021 ccgggacaac ggagccagag tctcactgct cgtgcgtcat cggccaggcg gagccgtact
     1081 acctgcgcag cgtgtttcgg gatatccggt cgcgcatcgt ctccccggtc ctcaacttca
     1141 gcacgggctg gagcgcgctg gttccgcaga tggcgagcgt cgcgacccag gtgggcgcca
     1201 cgatggcgga ggggctggcc gcggcggagc tggccgtcga gcaccagttg gagttcggcc
     1261 gcgcgcgcaa ggagatcggc gatcggctcc tcggcgagct ggaccggccg gatgccccga
     1321 tcgccgtggt cctgttcggg cgggcgtaca acgccttccc cggcgaggcc aatctgggga
     1381 tcccccagaa gttcgcctcc cgaggggtat ccgtggttcc gttcgattgc ctcccgacca
     1441 aggcggaaga gtcgatcgac ggcatgagct gggcgtccgg ccatgagatc atcaaagcgg
     1501 cgaggttcgt gaagggccac ccccgccttt tcggctgcta catcacgaac ttcagctgcg
     1561 gtccggattc cttcctggtc ggatacttcc gggacatcat gaagaccaag ccctccctta
     1621 ccctggagct ggactcccac agcgcggacg caggcatcaa cacgcgagtg gaggcgtttc
     1681 tggacatcat cgaacggtcc gtccggattg gggtccggga ctcccatcgg gagcccttcg
     1741 tgccggcccg ggtagagccg gggaagagag gcttccagtt cgtctgctcc gatggcaaac
     1801 gggtcccgct ttccgttgcg gagatcctca ttccctccat gggagacatc acctcccgcc
     1861 tgggggcctc ggcgcttcgc ggcatggggt tcagcgcgcg tcacgcgccc atccccgact
     1921 tcccggtcct gatgaccgga cgcaagaaca cctcctgcaa ggaatgcctc ccgatgattc
     1981 tcaccacggg cacgcttctc accgagttga aggaccgccc gggaagctca ccgctggtct
     2041 acttcatgcc ggcccagggc ggcaactgcc gcttctcgca gtacacggtc ttcctccaga
     2101 agctgatcgc ggagaagcgt ctagccaatg ttgccctgtg gtcgctgaac agcaacaacg
     2161 gctacggcgg cctcagcgta ccggcgctgc tgtccatcct gcggtccatc atcgtttccg
     2221 actgcctgga cgacatcaag aacgcggtcc tggcgctggc tcgggatcgg gagaagggga
     2281 tgaccgtttt cgaagaagcc ctgagccgga tcagccgttg catggagcgc ggctcccgag
     2341 gcctgatgcg gacgctccgc ggcgttgcca ggatgctctc ctccatcgag ctgaccgggc
     2401 cgctgcacca ggccaggacg gtgttggtag cgggcgaaat ctatatccgc aaggacaact
     2461 tctcatccga cgtgctcgtg gagcggctgg cccggcgcgg gatcgtctgc aggcgggcgc
     2521 ccataacgga atggctggcg tacgtggact actacgccca gcatctcatc cgctcgggca
     2581 tgagtctgtc cgagcgggtg tatctggtcg gcaagaagct gatccaggcc cgcatggaga
     2641 ggcgcatcaa gaaggcgctc gccgcatcgg ggctgtgcga gaacgaaccg gtggatatcc
     2701 ccgcgctcat ccgggtggga cgtcggttcg tgcgggagga gctgaccgga gagatcatcc
     2761 tggtgatcgg agcgttcttc cgggagatga tcgaccacat ccacggcctg gtatcgatcg
     2821 ggccgttcgc ctgcctgccg tcccgggtca tcgagtccat cctcagcgtg gagtcgaacg
     2881 tgcggaacaa cgagcggctg aagagcctgg agacctatga cgccattcgc cggttcagct
     2941 cgctgccctt cctggcagtg gagtgcgacg gcaacccgtt ccccccgatc atcgagacgc
     3001 gcatcgaggc gttcgcgctc caggtggagc gcgtccacgc ccaggctcgc gagagccagg
     3061 acgggcggca ccggtcctga ccggctcagc tctttcgccc cagccttacc gacaccacgg
     3121 cgaccaggag gaagtacagc gacttcaggc gccccatacc tcagcggcct gcctgtttgg
     3181 ctcgcagggc cgcggcttcc tcacgggacg gcccgtacac gcccggcacg gcgagccccg
     3241 cggatcgcat caggatggtc atctggcccc gatggtggat ctggtggcgg atgagcccct
     3301 ggagggcgac ggtgagcttg atcgtccggc cgaagagcgg gatctcggtg ccaagctgat
     3361 cgtccttgag ccgggcggaa gcctgctcgg ccatggagcg ggcggcccgc tcgtaggcat
     3421 ccgcgattgc tgcagccgtt gcgggcggct cggtcccccg gggaggcgcg tccaccgtca
     3481 ggcccgcccc ggtggccgtg agcccgatca tcactaccat gtgccacgcg atcttgccta
     3541 gcgtgttgga ttccgcgtcc gctttctgcg agagggaagc gtcggtaagc atgcgctcca
     3601 ccttgaggct gttcccgctt tcacgggtcc agtcctccac aaactcggaa atgctcgtgt
     3661 acatccgttc tcctcctggg cgccggccgc gagagccgcc cctcaaatga tctccaggta
     3721 gtcccgatcc ggcagcgccg ggaagggagc cgtgggcagc gtctggtacc agcaggcaac
     3781 cgaggctatg tcgtcctgca tgggaaggta gcggccgccg tttctccaac cgagtccctg
     3841 gatggtgacc ttgatatcct tctcgaaccg gatgggatcc ggtatgtgcc atcggtacat
     3901 ggcatggcga tgttgggacg cgtacagccc atcgggcttg atcacctggt gcatccccat
     3961 gaagggagtt gagtaaggga cgtagcgtcc gtccacgtcc cagttgtacg cgccgccgaa
     4021 gtagtcctcg gttcccgtcc cgcagatggt gggaaacg
//
LOCUS       DASRZS010000409         5267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3684952, whole genome shotgun sequence.
ACCESSION   DASRZS010000409 DASRZS010000000
VERSION     DASRZS010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3684952"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFH83_09960"
     CDS             <1..387
                     /locus_tag="VFH83_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6486733.1"
                     /translation="AGSFAAGVSIALPVADGGLRSAEAQQASDQIASLTAQRDQQQQS
                     ITIDVENALFGVADAKDRIELAQRNVQQAQGQYDLEKAKYAVGTETTLDVLTAFSALI
                     SAQVGLEQARNTYILALLNLDNVMGL"
     gene            404..1765
                     /locus_tag="VFH83_09965"
     CDS             404..1765
                     /locus_tag="VFH83_09965"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6486734.1"
                     /translation="MKTGVKVIIVVGAVVVVAAGAIVALRARADRRDEVSYVTRPAAM
                     ATVTATVSETGTVNPVNTVSVGCEVSGTIRSLGANFNDQVKKGQILATLDPTTYQAAV
                     DSAEATVRLDEATLNSAKVNVGKMKDLVDMANLTVQRDESLYQKGLITQSQMDTDTTA
                     AATANQDYLAAQAQVQVVLAQLDVAKAQLAQAQYNLSRAVIRSPMDGVVLARNVSVGQ
                     TVAASLQTPTLFSLATNLTDMQVDTSVDEADAGSVHTGDPAHFTVTSYPNQVFDGAVE
                     QVRVNPTVVQNVVTYDAVVAVHDTSGRLLPGMTAQVTIDVGTRTGVTAVPISALLYRP
                     LASSGGSGGSAGGVFGGLGRSAGLVQSSGPAASAQQVAGAPGSQVTVWILQNDQPQAR
                     SVIIGLSDGRNVEITSGDLRTGDLVIVAQRRGARPQNATPSHSPGAGAAGSSTIARAQ
                     GNP"
     gene            1762..2610
                     /locus_tag="VFH83_09970"
     CDS             1762..2610
                     /locus_tag="VFH83_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486735.1"
                     /translation="MSDESEATTMGTTTEEVLESAPQTATGLPRAEQPLIEVRDLTKV
                     YGEGPYAVGALRGVNLRVSHGESLAIMGPSGSGKSTFMHLLGCLDRPTSGSYRLAGQE
                     VSGLSRDQLALIRSRQIGFVFQSFNLLPRTTALENVELPMLYAGASQRARSQRARELL
                     EQVGLKDRVNHRPNELSGGQQQRVAIARALANGVPLLLADEPTGNLDSASSVEIMTLF
                     RNLNQESGITIVLVTHATEVAAWMERIVTFRDGRIIDDRPSREVLGEQGYPGSGQRRG
                     PAEVTA"
     gene            2607..3824
                     /locus_tag="VFH83_09975"
     CDS             2607..3824
                     /locus_tag="VFH83_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486736.1"
                     /translation="MNLMVLIRTAWKALRTNVSRSLLTMLGIIVGVGAVITSMAIGAG
                     AQAAVLKQIESLGANLVMITPGATTTGGVNLGSGSRTTLTLSDVAAISANVPDIGGVA
                     PYSQTGAQVVASGTNWSTVIGGTTPEWVSVENWTIAQGAFFTQDDVTRVAKVAVLGST
                     VASNLFPAGGAVGSMVVVKSVPFRVVGVLASKGQSGIGRDQDDVVVVPISTHQERLTG
                     QSWVNSIIISASTPDSVAGAIDASERLLRLMHHLTPGQGDDFTIRNIANVQQVLMAIS
                     QTQALLLAAVAVVSLVVGGIGIMNIMLVSVTERTREIGLRLAVGAKGRHILLQFLIEA
                     LTLACAGGLIGVGLGIGISYIVSLLGSWPTVVTGFSIVLGFASAAVTGMVFGFYPARR
                     AALLNPIQALRYE"
     gene            complement(3940..>5267)
                     /locus_tag="VFH83_09980"
     CDS             complement(3940..>5267)
                     /locus_tag="VFH83_09980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6486737.1"
                     /translation="MPELLQSYAMGETRHFEAHVDHSVIHLTIVPVAGRGHVDLYGRD
                     ITEERSLSAKFLQAQKMEAVGRLAGGIAHDFNNLLTVIAGYCELAEQEMPEDSPAGAL
                     INEIAKAAAQAARLTAKLLAFSRKQVLMPRVINPNALLKAAENMITRLVGEDIEVRIV
                     LSADAGNIMADPGQIEQVLMNLVVNARDAMPGRGKLTIETSNRTLDNEYALGHPGVMA
                     GDYVQITVSDTGHGMGEEVFSHLFEPFFTTKEPGKGTGLGLSTVYGIIKQSAGHITCY
                     SEPGQGTTFTLFFPRTIENRDATAAPDARIAMFRGNESILLVEDQEMVRRYMQMVLKN
                     NGYTVLASPGGAEALAESQRCRVDLLVTDVVLPQMSGTELAQKLRGACPALKVLFLSG
                     YAETAAVQQGVLEPGHNFLQKPFSSVELLKMIRDILDEHPGSNPLRKQG"
BASE COUNT          944 a         1700 c         1704 g          919 t
ORIGIN      
        1 gcgggaagct tcgctgccgg ggtttccatc gcattgccgg tcgcggacgg cggtctgagg
       61 agcgccgagg cgcaacaggc atcggaccag attgcctccc tcacggcgca gcgggaccag
      121 cagcagcaga gcatcacgat cgacgtggag aacgcgctat tcggcgtcgc cgatgccaag
      181 gatcggatcg agctggcaca acggaacgtc cagcaagcgc agggacaata cgatttggag
      241 aaagcaaagt acgcggtggg aacggagacg acgctggacg tgctcacggc attctccgcg
      301 ctcatcagcg cgcaggtggg tctggagcag gccaggaaca cttacatcct ggccctgctc
      361 aacctcgaca acgtcatggg gctttaggag aggaagggtt gcgatgaaga caggggtaaa
      421 ggtgatcatc gtcgtgggag cggttgtggt ggtggctgcc ggggccatcg tggcgctgcg
      481 cgctcgagcc gaccggcgcg acgaggtcag ctacgtcacc cggcccgccg cgatggctac
      541 cgtcacggcg accgtgagcg agacggggac cgtcaacccg gtgaacacgg tgtccgtggg
      601 atgcgaggta tcagggacca tccggagcct gggcgcgaac ttcaacgacc aggtgaagaa
      661 gggccagatc ctggccacgc tggatcccac cacgtaccag gcggccgtgg attccgccga
      721 ggcgacggtt cgcctggacg aggcgaccct caacagcgcg aaggtgaacg tcggcaaaat
      781 gaaggatctg gtagacatgg cgaacctcac tgtccagagg gacgagtcgc tctaccagaa
      841 gggcctcatc acccagagcc agatggacac ggacaccacg gcagccgcca ccgccaacca
      901 ggattacctg gccgcgcagg cgcaggtcca ggtggttctg gcccagctcg acgtggccaa
      961 ggcgcaactc gcgcaggcgc agtacaacct gagcagggcg gtgatccgaa gccccatgga
     1021 cggcgtggtg cttgcccgca acgtgagcgt cggacagacc gtcgcggcct ccctgcagac
     1081 tccgacgctc ttcagcctgg ccacgaacct caccgatatg caggtggaca cttcggtgga
     1141 cgaggcggac gcggggagcg tccacaccgg ggacccggcg cacttcaccg tgacctccta
     1201 tcccaaccag gtctttgatg gggccgtgga gcaggtccgg gtcaacccga cggtcgtcca
     1261 gaacgtcgtg acctacgacg ccgtggtggc cgtgcatgac acctcggggc ggctccttcc
     1321 cggcatgacg gcgcaggtga cgatcgatgt tggaacacgc accggagtga cggccgtgcc
     1381 gatctcggcc ctcctctacc ggcctcttgc gagctccggt ggaagcggag gatccgccgg
     1441 gggagtcttt ggcggtcttg gcaggagcgc cgggctggtg cagagctctg gacccgccgc
     1501 ctctgcgcag caggtcgcgg gcgcgccggg ctcgcaggtg acggtctgga tccttcagaa
     1561 cgatcagccg caggctcgat ccgtgatcat cgggctctcg gacggcagga acgtggagat
     1621 caccagcgga gacctgcgca cgggcgacct ggtcatcgtg gcgcagcgcc gcggcgcgcg
     1681 cccccaaaac gcgacgccga gccactcccc gggcgccggc gccgcgggat cctccacgat
     1741 cgcccgcgcc caggggaatc cgtgagtgac gagagcgagg caacgaccat gggcacgact
     1801 acagaagagg ttttggaaag cgcgcctcag accgcgacgg ggctgccacg ggcagaacag
     1861 ccgctgatcg aagtgcgaga cctgaccaag gtctatgggg aaggacccta cgcggtgggt
     1921 gccttgcgcg gggtgaacct gcgggtctcc cacggcgagt cccttgccat catgggaccc
     1981 tccggctccg gcaagtccac cttcatgcac ctcctgggat gcctggatcg gccgacatcc
     2041 ggctcctacc gcctggcggg acaggaagtc tcggggcttt cccgtgatca gctcgcgctg
     2101 attcggagcc gccagatcgg gttcgtgttc cagagcttca accttctccc tcgcaccact
     2161 gcgctggaga acgtcgagct gcccatgctc tacgccggcg cgtcacaacg ggcacgcagc
     2221 caacgggccc gcgagctcct ggagcaggtc gggctcaagg accgtgtgaa ccaccggccc
     2281 aacgagcttt ccggcggaca gcagcaacgc gtggccatcg ctcgcgctct cgcgaatggc
     2341 gttcccctcc tcctggccga cgagcccacc ggtaacctgg acagcgccag cagcgtcgag
     2401 atcatgaccc tcttccgcaa cctgaaccag gagtcgggga tcacgatcgt tctcgttacg
     2461 cacgcgaccg aggttgccgc ctggatggaa cggatcgtca ccttccgaga cgggcgcatt
     2521 atcgatgacc gcccttcccg tgaggtgctc ggggagcagg ggtatcccgg aagcggtcag
     2581 cgccgcggcc ctgcggaggt caccgcatga atctcatggt cctgatacga accgcgtgga
     2641 aggcgctgcg aacaaacgtc agccgctcgc tcctcaccat gctcggaatt atcgtcggcg
     2701 tcggagccgt aatcacctcg atggccatcg gagccggagc gcaggccgcg gtgctcaagc
     2761 agatcgaaag cctgggggcc aacctcgtca tgatcacgcc gggcgccacc accacgggcg
     2821 gagtcaacct gggaagcggc tcccgaacca ccctgacctt gagcgacgtc gcggccattt
     2881 cggccaacgt tccggatatc ggcggggttg ccccctattc ccagaccggc gcgcaggtgg
     2941 ttgccagcgg gaccaactgg agcacggtga tcggcggcac caccccggag tgggtgtccg
     3001 tggagaactg gacgatcgcc cagggagcct tcttcacgca ggacgatgtg acgcgggtag
     3061 ccaaggtggc cgtcctgggc agcacggtcg catccaacct cttcccggcg gggggagcgg
     3121 ttggcagcat ggtcgtcgtc aagagcgttc cgttccgggt cgtcggagtg ctcgcttcca
     3181 agggccagtc gggaatcggt cgagaccaag atgacgtggt tgtcgttccc atctcaacgc
     3241 accaggagcg gctgacgggg cagtcctggg tcaacagcat catcatctcc gcgtccacgc
     3301 cggattccgt ggcgggggcg atcgacgcaa gcgagcgtct gttgcgcctc atgcaccacc
     3361 tcacgccggg ccagggcgat gacttcacca tccgaaacat cgccaacgtt cagcaggtcc
     3421 tgatggccat atcgcagacc caggcgcttc tgctggccgc cgtggccgtc gtgtccctgg
     3481 tggtgggcgg catcggcatc atgaacatca tgctggtgtc cgtcacggag cgcacccggg
     3541 agatcgggct gcgcctggcc gtaggggcca aaggacggca tatcctgctg cagtttctga
     3601 tcgaggcgct cacccttgct tgcgcgggag gcctcatcgg ggtcggcctg ggaatcggga
     3661 tatcctacat cgtatcgctg ctcggaagct ggcccaccgt ggtcaccggg ttctcgatcg
     3721 tcctgggctt cgcatccgcg gccgtcacgg gcatggtgtt cgggttctac ccggcgcgtc
     3781 gtgcggctct cctgaatccc atccaggcgc ttcgctacga gtagcggcgt gcggaccggc
     3841 cggccgccgc ctgcgtggac ggcccggccc ggtccatctt aaggctgcct gtcacgagcc
     3901 ctatgacgaa gcgccatccc ttgtaggtcc acgggtgatc tatccctgtt tgcggagtgg
     3961 gttgctgcca gggtgctcat ccaggatgtc gcggatcatc ttgagcaact ctaccgagct
     4021 gaacggtttc tgcaggaagt tgtgaccagg ttccagtacc ccttgctgta cggcggcggt
     4081 ttccgcatac cctgacagga agagcacctt cagggccggg catgcgcccc gcagcttctg
     4141 cgccagctcc gtcccgctca tctggggtag cacaacgtcg gtcaccagga ggtcgacccg
     4201 gcaccgctgc gattcagcca acgcctcggc gcctccggga gaggccagga cggtatatcc
     4261 gttgttcttc agcaccatct gcatgtaccg acggaccatc tcctggtctt ccacaaggag
     4321 aatcgactca ttgccacgga acatcgcgat tctcgcgtcg ggcgcggcgg tcgcgtctct
     4381 gttctcaatg gtgcgcggga aaaagagcgt gaaggttgtc ccctgccctg gttcgctata
     4441 gcaggtgatg tgaccggcgc tctgtttgat gatcccgtac accgtggaaa gccccagccc
     4501 cgtccccttg cccggttctt tcgtggtgaa aaatggttcg aacaaatggg aaaacacttc
     4561 ctctcccatg ccgtgaccgg tgtcgcttac ggtgatctgc acatagtcgc cagccatgac
     4621 gcctggatgc cccaaggcgt attcgttgtc gagcgtccga ttggaggtct cgatcgtcag
     4681 ctttccccga cccggcatgg cgtcccgggc gttgacaacc agattcatga ggacctgttc
     4741 gatctggcca ggatcggcca tgatgttccc ggcatccgcg gacaggacaa tccttacctc
     4801 gatgtcctct ccgacgagtc gagtgatcat gttctccgct gccttcagaa gggcgttcgg
     4861 attgatgacc cgcggcatca gaacctgctt cctgctaaac gccaggagct tcgccgtaag
     4921 acgggctgcc tgcgctgccg cctttgcgat ctcattgatc agcgcccccg ccggactgtc
     4981 ttccggcatc tcttgttccg caagctcgca gtagcctgca atgacggtga gcaggttgtt
     5041 gaagtcgtgc gcgataccgc cggcaagccg tccgacggct tccattttct gcgcctgcag
     5101 gaacttcgcg ctgagcgacc tctcctctgt gatatccctg ccataaaggt ccacgtgccc
     5161 acggccggca accgggacaa tcgtgagatg gatgacgcta tgatccacgt gcgcttcgaa
     5221 gtgacgggtc tcgcccatgg cataggactg aagaagctcc ggcatgt
//
LOCUS       DASRZS010000410        11935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3302627, whole genome shotgun sequence.
ACCESSION   DASRZS010000410 DASRZS010000000
VERSION     DASRZS010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11935
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3302627"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="VFH83_09985"
     CDS             <1..1474
                     /locus_tag="VFH83_09985"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HET6486738.1"
                     /translation="EETIRLGADPASAATWLAADVKKHLNRSGSRLDESPLTPKRLAQ
                     MLGLLSERRIHGRIAKSVLEAMFQENRDPVDIIREKGWEQITDPAVLGGYLEKVMQAN
                     AAAVSAIRSGDHRPTTFLIGEIMRETSGRADPALVQALVKKKLSVSLIQILSLGGAIA
                     GTISEQGDVVAGDPSEILRLLSASDGLDGARSGTSSGSLPNLALEGGEVGRILSEEIT
                     PEDWARLASGIDDHLRRGKASGIVVTHGTDTLAYTAPLVFWLFPRPAVPIVIAASLQP
                     PAAPGSDAAATLRLAVRTVSERPPGVYVVFGGEVLSPLNLKFERMAAGGFRNWNLPQP
                     CHTAAPLFDDLNLAADRDGLRNRLEAAINSTVILKMYPGMRSDHLVELMDRGVKNFVL
                     ELYDTGTANLRDSPYSLRRVLIPGRERGVHFFCTSQQEGKVDFSSYATSHELWREGAV
                     PMGTLTTESAWTKLVVCSAFSDTEEELLARMEHGNANACL"
     gene            1455..2729
                     /gene="aspS"
                     /locus_tag="VFH83_09990"
     CDS             1455..2729
                     /gene="aspS"
                     /locus_tag="VFH83_09990"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HET6486739.1"
                     /translation="MRTLVSELSSRIGETVTLSGWVHRVRDLGGVSFVVLRDRSGMAQ
                     LVFDSRPEVTPESVIQVEGAAAANEKAPGGVEVRVARLTMIAPAAPDLPFPVNQDPGK
                     TSLESILDNRMLSVRNPAILSIFRLQATILRCFAEHLRGEGFTEIKTSKLVGTGTEGG
                     TNLFAVEYFDSKVYLSQSPQLYKQVMIASGLERVFEIGPAYRAEKHDTPRHINEFESL
                     DVEMGFIESEQDLMDLETRILAAIFDGIRRNNQRDLEAWKAEAPDPSLCPRIPRVSHD
                     EAREIVSKETGKKVYEIGPEGERALCDWAGRTAGIEAAFVYAYPRRSRPFYTHPAENN
                     KTRAFDLLFRGLEITSGGMRINQHDMLVESAKTFGLDPAGLSDYLTIFRYGCPPHGGF
                     AIGLERLTQKVLGLGSVKEAALFPRDRKRTRP"
     gene            2837..4039
                     /locus_tag="VFH83_09995"
     CDS             2837..4039
                     /locus_tag="VFH83_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6486740.1"
                     /translation="MRKIAKWILVVAILSVALAAYAGGTTEKTTGSLTVGLLLVGPYN
                     DHGWSQAHYDAMQYVMGKVPGVKMVYIDKANSADRPGTTVSQLAESLLSQGAKLIIFS
                     SDDMSDEAVKFAQAHTDIPVIMASGSQVWKDGKNYQSIPNMVNIMGRMEYTKMIAGVA
                     AAMTTQTGEIGYLGPLINDETRRLASSVYLGARYAWVNYLKKDPAQLKFKVTWIGFWF
                     NIPGVTSDPSQVADDFFNSGYDVVVSGIDTTEATVEAVKLAGQGKKVWAIPYDYSKAT
                     DEGPSVALGVPYFNWGPAYVNAIKAAMAGKWQSHFEWNGPDWKNINNPDTSAVGFNKG
                     AALSKTASDAVDKFVGELAGGLNLWKGPLALQDGSQYLPGGAAATDQQVWYLPQLLQG
                     MEGQSVSK"
     gene            4193..5686
                     /locus_tag="VFH83_10000"
     CDS             4193..5686
                     /locus_tag="VFH83_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009556097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6486741.1"
                     /translation="MQVELQTIHKSFGSVRANDGISLTIPASGIQGILGENGAGKSTL
                     MKILSGFFHPESGEIRLDGVPVLLSSPADAIRKGIGMLHQDPMDFPSLKLVDDFTLGR
                     GGGLFPDRRKADQELRRLSAQFGFDLDPETYVDTLTVGERQQLEILRLLSMGARVLIL
                     DEPTTGISLPQKEKLFSTLKQLAAQGMSVVFVSHKLDDVEKLCDRVAVLRHGRLVGTA
                     EPPYVTRELVRMMFGRDVASGARSELEPGTAVLALHRVCVEGIRLQIRDVSFQARAGE
                     VIGLAGMEGSGQSVLLSACAGLNRPVAGRIRLSDRDVTGQSHHLFKRHGVAYLPASRL
                     EQGLVPGLTLTEHFVLSEGARGVFIDRRRAAEIAEERIRSYNIKGSPSHRVESLSGGN
                     QQRMLLALLRSPLSLILLEQPTRGLDIESTIYIWGKLKERCSQGAAIVFISSDLDEVL
                     QYSDRVLVFFAGKVSPPLRADRLTVERLGELIGGKGWERLEPEAARA"
     gene            5679..6716
                     /locus_tag="VFH83_10005"
     CDS             5679..6716
                     /locus_tag="VFH83_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015415996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486742.1"
                     /translation="MRDTPLSGVLLQIAAVLVALVLTTIVLLASKAPPLSAYWNIALG
                     AAGSWEVLANVLVSWVPLLIVTSGLLLTFTVGLWNIGIEGQITLGAIFATWALRLVQG
                     GSMDPTIAITLSILFGMVGGSLWAMVAGALKTFGGVNEIFGGLGLNFVASALNLWLIF
                     GPWKRPGVASMSGTVPFPQTLWLPTLTAQSRLSPAALILAILVIAGAFVLIQGTYFGL
                     RLKAVGRNPNAAYLMGIPTWQHMMISFALCGLLAGLAGAVQVTSVYHRLIPSISSGYG
                     YLGLMVAMLVNFKAALVAPVALFFAALNVGSIQLPIVLRLDSSLSGVLQGSLVLFVLL
                     GQGVRQRFWKG"
     gene            6723..7721
                     /locus_tag="VFH83_10010"
     CDS             6723..7721
                     /locus_tag="VFH83_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015164826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486743.1"
                     /translation="MSPDTVLVGLAGVLAMAGPILFGVIGETICERAGVINLSMNGTI
                     LLSAMTGFAVALASKSPSLGFLAGAAVGAAVALVVAFASITLRQSQVAVGFVLTLLCR
                     DLSYFLGNPIMGETGPRLTIAPIPLLRDLPILGPLLFRQDIMTYLSFLIVVGAWIFIY
                     RTRPGLVLRGIGDRPAAAYTRGASVNRLRYLYTGIGGAFAGLVGPIYSLSVKAGWKGT
                     ISGLDGIGWIVLAITIFGGWNPFRAAFGAYLFSILQWLGLVLQPALPGIPSQVLQVAP
                     FPLMILTLLLVNIGNAEWVERSLARLPAPARRAVTRLLRLLNATPPAALGTPFEEE"
     gene            complement(7777..9144)
                     /locus_tag="VFH83_10015"
     CDS             complement(7777..9144)
                     /locus_tag="VFH83_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET6486744.1"
                     /translation="MNLVSINPATGETVQTYPETTEQRVSEILDSAVRAQVPWSRVPL
                     DGRAERMKAMASALRQAQEPAARLMAVEMGKPISQGRAEVEKCAWLCEYAAENAEGLL
                     RSIDVGTDARKSYVAMRPLGVILAVMPWNFPYWQIFRAAVPALLAGNTMVLKHASNIC
                     GCSLAIEELFRAAGFPAGAFSSLLLDSRKVEAIVKDPRIQAVTLTGSTDAGRAVARAA
                     GETIKKCVLELGGSDPYLVLEDADLDLAARTCAASRLINSGQSCIAAKRFIVVESVLK
                     SFEERFIREMTAFRVGDPLDESTRVGPLARRDLRDTVSRQVERTVSQGARVLLGGPGA
                     DGKGFYYPATILGGVRKGMTAFDEEVFGPVASVVSAGSEGEAIRIANDSSFGLGAAVF
                     TRDSQRGERIAAIELEAGNCFVNALVRSDPRLPFGGIKESGFGRELGSFGIHEFVNIK
                     TVYVG"
     gene            complement(9141..9932)
                     /locus_tag="VFH83_10020"
     CDS             complement(9141..9932)
                     /locus_tag="VFH83_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET6486745.1"
                     /translation="MIEPGLVSVTFRKLHPSEIVALAARTGLSVIEWGGDIHVPHGQV
                     GRAREVRRMTLEAGLRVASYGSYYTLGSRKGPAFEAVVDTALALGAPVIRVWAGERGS
                     AEADDAYRHLVADDSHRTAELAGAAGLVTAFEFHHGTLTDTAASARELLERVNHPAAR
                     TYWQPATELDGSSNEAGLGLILSWLVNVHVYQIHANGQERLPLSDGAEPWSHYLSMLA
                     VNGRPHYAMLEFVRDDSPDAFVEDAADLRRWVAAANTSRMAPEDL"
     gene            complement(9941..10837)
                     /locus_tag="VFH83_10025"
     CDS             complement(9941..10837)
                     /locus_tag="VFH83_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486746.1"
                     /translation="MIFLTAATLFSAAFNLLMRFGQDRKCDMTALGAVNYITAALYNI
                     VVLSVTGALTAAGHPGQGPHAGSLLVGVAGGCAFVTTYFLLFHHLRRRGVTITMAVTR
                     LAALFPVVVSLVVWRDRTNAVQIAGGAIALVSLPLLSVRPSQAGEGRNRGGVLLMSAL
                     FVAQGLCLLAPRVFNQTGIRGEDAYFLAALFGTAAIASSSGWGVQSVRSRRERRAPAG
                     PVLVTVILGIVLGLTNTFASQFLVSSLLDVPSILVFPFYSTVGLLITVVFSRIVWRET
                     INRLETAGIVMACAAVVLMNLA"
     gene            complement(10834..>11935)
                     /locus_tag="VFH83_10030"
     CDS             complement(10834..>11935)
                     /locus_tag="VFH83_10030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6486747.1"
                     /translation="LPNLGVAPAQVPAWTGRVTALAGLVGIPLLPLWGALADRFSRQP
                     VIVRSFAVHLLSGILMLLAGNLWVFAMGRALTGFALGNSGLMMTTLSERAPRARTGFA
                     LAIMNSAAPIGAFAGPLFGGPAVDRWGFPTLLAADAGLMLVVIVALSVGYRDTFRAAQ
                     RKPILRMALESVGIVIRSALLRSLFAALFLLFAGWMLVMTYVPLVVTGIYRGSNPATV
                     VGLVLGLGGLSALAVGPLMGALADRLGHWQVLFYGAALSTVLWPVPMFTTGIVAFAMT
                     WGIVNGISSGVFAVSFVVLSESSPQAVRGRVMSFAYLPVNLGIFVGPAIGSALSRMNV
                     KLVFPTAAVLTGIGILVLIGTARLGRERLPSS"
BASE COUNT         2085 a         3921 c         3857 g         2072 t
ORIGIN      
        1 cgaagagacc attcgtctcg gcgcggaccc tgccagcgcg gcgacctggc tggccgcgga
       61 cgtgaagaaa cacctcaacc ggtcgggctc ccggctggac gagagcccgc tgaccccgaa
      121 acgcctggcc cagatgctcg gtctgctatc ggaacggcgg atccacggcc gcatcgccaa
      181 gtcggtgttg gaggccatgt tccaggagaa ccgggacccg gtcgatatca tccgtgagaa
      241 gggctgggag cagatcaccg accccgccgt cctgggaggc tacctcgaga aagtgatgca
      301 ggccaacgcg gccgccgtga gcgccatccg gtcgggagac caccggccta ccacgttcct
      361 catcggcgag atcatgcggg agacctccgg gcgcgctgac cccgccctgg tccaggcgct
      421 ggtcaagaag aagctttccg tttccttgat ccagatcctc tctctgggcg gagctattgc
      481 cggcacgatc agcgagcagg gggatgtggt ggcaggagat ccttcggaga tcctgcgcct
      541 cctctcagcg tccgacggtc tcgacggagc ccgctcagga acctcgagcg gatccctgcc
      601 caatctggcc ctggagggag gcgaggttgg cagaatcctg agtgaagaga tcacccccga
      661 ggactgggcg cgcctggctt cggggatcga tgatcacctc agacgcggga aggcgtcggg
      721 gatcgtggtg acccacggta ccgacacgct ggcctatacg gccccgctgg tgttctggct
      781 cttcccgcgg cccgcggtgc cgatcgtgat cgccgcgtcg ctccagccgc cggcggcgcc
      841 cggcagcgac gcggccgcca ctctccgcct cgcggtccgc accgtctcgg agcgtcctcc
      901 gggcgtatac gtggtcttcg gaggagaggt gctctcgccg ctcaacctga agttcgagcg
      961 catggccgcc gggggattcc gcaactggaa ccttccccag ccgtgtcaca cggccgcccc
     1021 cctcttcgac gatctgaacc tggccgcgga ccgcgacggg ctgcgcaacc gcttggaggc
     1081 cgccatcaac tcgacggtca ttctgaagat gtaccctggg atgcgctccg accacctggt
     1141 ggagctcatg gaccgggggg tgaagaactt cgtcctcgag ctctacgata ccggcacggc
     1201 aaacctgaga gactcgccct actcgctgcg ccgggtcctg atccccggac gagagcgcgg
     1261 ggtgcacttc ttctgtacct cccagcagga aggcaaagtg gacttctcca gctacgcgac
     1321 ttcccacgag ctgtggcgtg agggcgcggt gcccatgggt acgctgacta cggagtccgc
     1381 ctggaccaaa ctggtggtct gctctgcctt ctcggatacc gaggaggagc tactggcccg
     1441 gatggagcac ggcaatgcga acgcttgtct ctgagctttc ctcccgaatc ggcgagactg
     1501 tgacgctttc ggggtgggtg caccgggttc gtgacctggg cggggtctcc ttcgtggtcc
     1561 ttcgggaccg aagcgggatg gcccaactcg tgttcgattc tcgccccgaa gtgaccccgg
     1621 aatccgtgat ccaggtggaa ggcgcggcgg cggccaacga gaaggcgcct ggaggcgtgg
     1681 aggtacgcgt cgctcgcctc accatgatcg cgcctgcggc tccggacctc cccttccccg
     1741 tcaaccagga ccccggcaag acctccctgg agtccatcct cgacaaccgg atgctttccg
     1801 tccgcaatcc cgccatcctg tcgatctttc gactccaggc cacgatcctt cgatgttttg
     1861 ccgagcacct ccgaggtgag gggtttaccg agatcaagac ctccaagctg gtcggtaccg
     1921 gcaccgaagg gggtaccaac ctgttcgcgg tcgagtactt cgacagcaag gtctatctgt
     1981 cccagtcccc ccagttgtac aagcaggtga tgatcgcgtc cgggctcgaa cgggtgttcg
     2041 aaatcggccc ggcttaccgc gccgagaagc acgatacccc ccggcatatc aacgagttcg
     2101 agtccctgga cgtggagatg gggttcatcg agagcgaaca ggacctcatg gacctggaga
     2161 cccggatcct ggcggccatc ttcgatggca tccgccggaa caaccagcgc gatctggaag
     2221 cctggaaggc ggaggcgccg gacccgagcc tgtgcccccg gatcccgcgg gtttcccacg
     2281 acgaggctag agagatcgtc tccaaggaaa ccggcaagaa ggtgtacgag atcggtccgg
     2341 agggcgagcg agccctatgc gactgggccg gtcggacggc cggcatcgag gcggcattcg
     2401 tctacgcgta tccgcgccgc tcgcggccct tctatacgca ccctgctgaa aacaacaaga
     2461 cgcgcgcctt cgatctcctc ttccggggac tggagatcac ctcgggcggg atgaggatca
     2521 accagcatga catgctcgtg gagagcgcga agaccttcgg tcttgacccg gccgggctgt
     2581 ccgactacct gaccatcttc agatacggat gtccgcccca cggcggcttc gccatcggcc
     2641 tcgagcggct cacccagaaa gtgctgggcc tgggcagcgt caaggaggct gcgcttttcc
     2701 cgagggaccg caagcgaacc agaccgtagc cggcgttccg tgccctgggc tcggagggcc
     2761 gatcggtctc cgacttgagg ctcggtcggc ggcgggtgta tactcgcccg taaccagaac
     2821 acgataagga ggggtcatgc ggaagatcgc aaagtggatc ttggtcgtag ctattctcag
     2881 cgtcgcgctc gcggcctatg cgggcgggac gaccgagaag acaacgggga gcctcaccgt
     2941 gggactcctg ctcgtcgggc cgtacaacga tcacggctgg agccaggcgc actacgatgc
     3001 catgcagtac gtcatgggca aggttcccgg cgtcaaaatg gtctacatcg acaaggccaa
     3061 ctcggccgac cggccgggga ccacggtttc ccagctcgcg gagagtttgc tgtcgcaggg
     3121 ggcgaagctc atcatcttca gctccgacga catgagcgat gaggcggtga agttcgccca
     3181 ggcccacacg gacatccccg tgatcatggc ctccggcagc caggtctgga aggacggcaa
     3241 gaactaccaa agcatcccca acatggtgaa catcatgggc cgcatggagt acaccaagat
     3301 gatcgccggg gtagccgccg ccatgacgac ccagaccggg gagatcggct atctgggccc
     3361 cctcatcaac gacgagacgc gccgtcttgc ctcttcggta tacctgggcg cgcggtacgc
     3421 ctgggtcaac tacctcaaga aggatcccgc tcagctgaag ttcaaggtca cctggatcgg
     3481 gttctggttc aacatcccgg gcgtcacctc ggacccgtcc caggtcgcgg atgatttctt
     3541 caatagtggg tacgacgtgg tggtctcggg aatcgacact acggaggcca cggtggaagc
     3601 ggtgaagctc gccgggcagg gcaagaaggt gtgggccatc ccgtacgact actccaaggc
     3661 tacggatgaa ggcccttcgg tcgctttggg tgttccctac ttcaactggg gcccggctta
     3721 cgtgaatgcc atcaaggccg ccatggcagg caagtggcag tcccatttcg agtggaacgg
     3781 gccggactgg aagaacatca acaaccccga cacgtcggcg gtcgggttca acaaaggcgc
     3841 cgccctttcg aagaccgctt cggatgccgt ggacaagttc gttggagagt tggccggagg
     3901 gctgaacctc tggaagggac cgctcgcgct ccaggatggc agccagtacc ttcctggcgg
     3961 agccgccgcg accgaccagc aggtctggta cttgccgcag cttctgcagg gcatggaagg
     4021 ccagagcgtt tcgaagtagt ccgtccgtgg gatagcgtcg aggggcggga gaggcagcgc
     4081 gggtctccgc ggaggcccgc ccctcgttct tccggctggc gcccgcggga cccgcggtca
     4141 cggctgcacg aaagcagccg cggtccgagc gggtgccgca gatcaggttc gcatgcaagt
     4201 cgagctccaa accatccaca agagcttcgg aagcgttcgc gccaacgacg gcatttccct
     4261 gaccatcccg gcgtcgggca tccagggcat tctcggggaa aacggcgccg ggaaaagtac
     4321 cctgatgaag attctctcgg ggtttttcca tccggagagc ggggagatcc gcctggatgg
     4381 cgtgccggtc ctcctgagct cccccgcgga tgccatccgc aagggcatcg ggatgctcca
     4441 ccaggatccc atggatttcc cttccctgaa gctagtggac gacttcacgc ttggaagagg
     4501 aggtggcctc ttccccgacc gcagaaaggc ggaccaggag cttagaaggc tctccgccca
     4561 gtttggcttc gacctggacc cggaaaccta cgtcgacacc ctcacggtcg gggagcggca
     4621 gcaattggag atcctgaggc tcctctccat gggcgcccga gtcctgatcc tggacgaacc
     4681 gacgaccggg atcagcctcc cacagaaaga gaagcttttc tccacgctga agcagctcgc
     4741 ggctcagggg atgagcgtgg tcttcgtatc gcacaagctc gatgatgtgg agaagctctg
     4801 cgaccgggtc gcggtgttgc gccacggccg gctggtggga accgcagagc cgccgtatgt
     4861 cacgcgcgag ctcgttcgca tgatgttcgg ccgcgacgtt gcctcaggcg cccgcagcga
     4921 gcttgagccg ggtaccgccg ttctggcgct gcaccgggtg tgtgtggagg ggatccggct
     4981 ccagatccga gacgtgagct tccaggcacg ggccggcgag gtgattgggc tggcgggcat
     5041 ggaggggtcc ggtcagtcgg ttctcctgtc ggcctgtgcc ggactgaacc gccccgtggc
     5101 cgggcgcatc cggctctccg accgggacgt gacgggtcag tcccaccacc tcttcaagcg
     5161 gcacggggtt gcctatctac cggcatcccg tctggagcaa gggctggtcc cggggttgac
     5221 cctcaccgag cacttcgtcc tgtcggaggg agcgcggggc gtctttatcg atcgtcgccg
     5281 agccgcggag atcgccgagg agcgcatccg ctcctacaac atcaagggct cgccatccca
     5341 ccgcgtggaa agcctctccg gtggaaacca gcaacggatg ctccttgcgc ttcttcgtag
     5401 cccactgtcc ctcattctcc tggagcaacc cacccggggc ctggacatcg aatccacgat
     5461 ctacatctgg ggcaagctca aggaacgctg ctcccagggt gcggccatcg tgttcatctc
     5521 ctcggacctg gacgaggtgc tccagtacag cgaccgggtg ctggtcttct tcgccgggaa
     5581 agtctcccct ccccttcgcg ctgatcgtct cacggtggaa cggctcggtg agctgatcgg
     5641 cggcaaaggt tgggaacggc tggagccgga ggccgcgcgt gcgtgacacc ccgctttccg
     5701 gcgtcctcct tcagatcgcc gcggtcctgg tggccctggt tctcacaaca atcgtgctgc
     5761 tggcctccaa ggcgcctccc ctctccgctt actggaatat cgccctggga gcagccggtt
     5821 cctgggaggt cctggccaac gtcctggttt cctgggtgcc tcttctgatc gtcacctcgg
     5881 ggctcctcct cacgttcacc gtgggcctgt ggaacatcgg tatcgaaggt cagatcacgc
     5941 ttggcgccat cttcgcgacc tgggcccttc gcctcgttca gggcggaagc atggatccaa
     6001 cgatcgcgat caccctcagc attctcttcg ggatggtcgg cggctccctc tgggccatgg
     6061 tggccggggc gttgaaaacc tttggggggg tcaacgagat cttcggcggg ttgggtctga
     6121 acttcgtggc atccgcgctg aacctgtggc tgatctttgg accctggaag cggccggggg
     6181 tcgcttccat gtcgggaacg gttccgtttc cccagaccct ctggcttcca acgctcacgg
     6241 cccagtctcg gctgagtccg gcggcactga tcctcgctat cctggtgatc gccggggcct
     6301 tcgtcctgat ccagggcacg tacttcggcc ttcggctgaa ggcggtgggc aggaacccaa
     6361 acgccgcgta cctgatggga atcccgacct ggcagcacat gatgatctcc ttcgcgctgt
     6421 gcggcctgct tgccggtctg gcgggagcgg tccaggtcac ttcggtgtac catcggctga
     6481 ttccctcgat ctcgagcggc tacggctatc tcgggctcat ggtggcgatg ctggtgaact
     6541 tcaaggcagc gttggtggca ccggttgcgc ttttctttgc cgctctcaac gtgggcagca
     6601 tccagctccc tatcgtgctc cggctcgact cgtccctctc cggcgtattg caggggtccc
     6661 tggtgttgtt cgtcctcctg ggtcagggcg tccgccagcg gttctggaag ggatagacgg
     6721 gcatgagtcc ggacacggtc ctggtagggt tggctggggt cctggcgatg gcggggccca
     6781 tcctcttcgg cgtgatcggg gagacgatct gcgagcgggc cggtgtgatc aacctctcca
     6841 tgaacggcac gatcctgctt tcagccatga ccggcttcgc ggtcgccctc gcctcgaaga
     6901 gcccgtccct tgggttcctc gccggcgcgg cggtcggcgc cgcggtggcg ctcgtggtgg
     6961 cgtttgcgag catcacgttg cgccagtccc aggtggctgt cgggttcgtc ttgactctcc
     7021 tctgccgcga cctgtcctac ttccttggta accccatcat gggcgaaacg gggccgcgtc
     7081 tcaccattgc cccaatcccc ttgcttcgcg acctgccgat cctggggccc ctcctgttcc
     7141 gacaggacat catgacgtac ctgagtttct tgatcgtggt aggcgcctgg atcttcatct
     7201 atcgcacgcg gccgggcctt gtgctgcggg gaatcgggga tcggcccgct gctgcctata
     7261 cccgcggcgc cagcgtgaac cgcctgcgct atctgtatac cggcatcgga ggcgctttcg
     7321 ccggcctggt cggtcccatc tactccctgt cggtcaaggc cggctggaaa ggaaccatct
     7381 ccggcctcga tgggatcggc tggatcgtgc tggccatcac gatcttcgga ggctggaacc
     7441 cgtttcgagc cgcctttggg gcctacctgt tctccatcct tcagtggctg gggctggtcc
     7501 tccagccggc gctgcccggt attccttccc aggtcctcca ggtggcgcct ttcccactca
     7561 tgatcctgac cttgcttctg gtcaacatcg ggaacgcgga atgggtggag cggtcgttgg
     7621 cccgattgcc cgcgccggcc cgtcgcgcgg tgacccgctt gctgcgtctg ttgaacgcca
     7681 ctccacccgc ggcgctggga acgccgttcg aggaggaata ggcgcccgga gagaaaccgg
     7741 gtcgggtcga aagcgacccg aagcgcgtcg gacggtctat ccgacgtaca cggtctttat
     7801 gttcacaaac tcgtggatgc cgaaggaccc gagctcgcgg ccaaagccgc tctctttgat
     7861 tccgccgaac gggagccgag gatccgaccg gaccagcgcg ttcacaaagc agttcccggc
     7921 ttccagctca atggcggcga tgcgctctcc ccgctggcta tcccgggtga acaccgcggc
     7981 gcccaagccg aacgaagagt cgttggcgat ccggatggcc tccccctccg agccggctga
     8041 gaccacggac gctactggtc cgaagacctc ctcgtcgaac gccgtcatgc ctttgcgcac
     8101 cccgcccagg atcgtggccg ggtagtagaa ccccttgccg tccgcgccgg ggcctcccag
     8161 gagcacgcgc gccccttgag acacggtgcg ctctacctga cgggagaccg tgtcccggag
     8221 gtcccggcgg gccagcggac cgactcgggt ggattcgtcc aacggatccc cgacccgaaa
     8281 ggccgtcatc tcccggatga accgctcctc gaaggatttc agaacgctct ccacgacgat
     8341 aaaccgcttg gctgcgatgc agctctggcc cgagttgatg agccggctcg ccgcgcaggt
     8401 tctcgccgca agatccaggt ccgcgtcttc cagcacgaga taggggtcgc ttccccccag
     8461 ctccagcacg cacttcttga tggtctcccc cgcggcacgc gccacggcgc gacccgcgtc
     8521 cgtgctcccc gtaagcgtga ccgcctggat ccgcggatcc ttcacgatgg cctcgacctt
     8581 gcgggagtcc aggagcaggc tgctgaaagc gcccgccggg aagccggcag cccggaagag
     8641 ctcttcgatg gcgagcgaac atccgcagat attggacgcg tgcttgagga ccatggtgtt
     8701 gcccgctaga agggccggca ccgctgctcg gaagatctgc cagtacggga agttccacgg
     8761 catcaccgca aggatgactc ccagcggccg catggccaca tagcttttgc gggcgtcggt
     8821 tccgacgtcg atcgagcgca gaagcccttc cgcgttctcg gcagcgtact cgcacaacca
     8881 ggcgcacttc tccacttccg cccgcccttg ggagatcggc tttcccatct cgaccgccat
     8941 cagccgggcg gcaggttcct gagcctgccg aagcgcagac gccatggcct tcatccgctc
     9001 ggctctgccg tccagaggca ctcggctcca aggcacttgc gcccgcaccg cggagtccag
     9061 gatctcggac acccgctgtt ccgtggtctc aggatacgtc tgtacggtct ctcccgtggc
     9121 ggggttgatc gaaacgaggt tcataagtcc tccggtgcca ttcggctggt attcgccgcg
     9181 gcgacccagc gtctcagatc tgccgcgtcc tcgacaaatg cgtccgggct atcatcccgg
     9241 acgaactcca gcatcgcata gtgtggtctg ccgttgaccg ccagcatgga aagatagtgt
     9301 gaccagggct cggctccgtc cgacaaggga agccgctcct gcccgttcgc atggatctgg
     9361 tagacgtgga cgttgacgag ccagctcaag atgagaccca gcccggcctc gttggatgat
     9421 ccgtccagct ccgtggccgg ctgccagtag gtcctggcgg ccggatgatt tacgcgttcc
     9481 aggagctcgc gcgcggaggc ggccgtgtcc gtgagtgtcc cgtgatggaa ctcgaacgcc
     9541 gtgaccagac ctgcggcgcc tgccaactcg gcggttcggt ggctatcgtc ggccacgagg
     9601 tgccggtacg cgtcgtccgc ctccgctgag ccgcgttcac cggcccagac ccggataacc
     9661 ggcgccccca gcgccagagc cgtgtctacc acggcctcga aggcaggtcc tttccggctg
     9721 ccgagggtgt agtaggagcc gtaggaagcc acccgcaacc ccgcttcgag ggtcatccgc
     9781 cttacctcgc gggcgcgacc gacctggcca tgaggcacgt ggatgtcgcc gccccattct
     9841 atgacggaga gtcctgttct ggccgccagc gccacgatct ccgaggggtg cagcttgcgg
     9901 aaggtcacgg aaacgaggcc gggttcgatc atggtatccc ttacgcgagg ttcatgagca
     9961 caaccgcggc gcacgccatc acgatccccg cggtttccag acggttgatc gtttcccgcc
    10021 agacgatccg cgagaagacc acggtgatca gcagtcccac ggtactgtag aaagggaaca
    10081 cgagaatgct cgggacgtcc agcagggagc tcaccaggaa ctggctggcg aaagtgttcg
    10141 tgagccccag gacaatcccc aggatcacgg ttaccaggac cggtccggcc ggagccctcc
    10201 gctctctccg actgcgcacg gactgcacac cccagccact gctggatgcg atggcagccg
    10261 tgccgaagag tgctgccagg aagtaggcgt cctcgccgcg gatgccggtc tggttgaaca
    10321 cgcggggagc caggagacag agaccctggg ctacgaagag agcagacatg agaaggactc
    10381 cgcctctgtt gcggccttcc cccgcttggg aaggccgcac gctcaggaga ggcaacgaca
    10441 ccagcgcgat agctcccccg gctatctgga ccgcattggt ccgatcacgc cacaccacca
    10501 gggagaccac caccgggaac agcgcggcca ggcgggtgac ggccatcgtg atggtcaccc
    10561 cccgtcggcg caggtgatgg aacaggagga agtaggtcgt gacaaacgcg cagccgccgg
    10621 cgacgcccac cagcaggctg ccggcatgcg ggccctggcc gggatgtccg gcggccgtca
    10681 gcgcgccggt gaccgacaga accacgatgt tgtagagggc cgctgtgatg tagttgaccg
    10741 cgccgagggc ggtcatgtcg cacttgcggt cctggccgaa acgcatgagc aggttgaacg
    10801 ccgctgagaa cagagtggcc gccgtgagga agatcacgag gagggtagcc tttcccgacc
    10861 caggcgggcg gtcccgatca ggaccaggat gccgattccg gtgagcacgg ctgctgttgg
    10921 gaataccagc ttgacattca tgcggctgag cgcgctgccg atcgcgggcc ccacgaagat
    10981 cccgaggttt accggcaggt aggcgaagga catcacccga ccgcgcacgg cctgcgggga
    11041 ggattccgag agaaccacga aggagaccgc gaacacgccc gaggagattc cgttgacgat
    11101 cccccaggtc atggcgaatg caacgatgcc ggtggtgaac atcggcacgg gccacaaaac
    11161 ggtggagagc gcggctccgt agaaaagcac ttgccagtgg ccaagacggt ccgccagagc
    11221 ccccatcagc gggcccaccg caagcgcgct caggcccccc aggccaagga ccagcccgac
    11281 cacggtagcg gggttcgacc cgcggtagat gccggtgacc accaggggga cgtacgtcat
    11341 gacgagcatc cacccggcaa agaggaggaa cagagcggcg aagagcgacc gcagcagagc
    11401 agagcgaatg acgatgccga cggattccag ggccatacgc aggatcggct tccgttgggc
    11461 cgctcggaac gtgtcgcggt aaccaacgct caacgccacg atgaccacga gcatgagccc
    11521 cgcatccgcg gccagaagcg tggggaaccc ccagcggtcc accgccgggc ctccgaacag
    11581 cggtcccgca aaagcgccga taggggcggc gctgttcatg atcgccaagg cgaagccggt
    11641 cctggcgcgg ggcgcccgct cggacaacgt ggtcatcatc aggccgctgt tgcccagcgc
    11701 gaagccggtg agcgcgcggc ccatcgcgaa aacccacaag ttgccggcca ggagcatcag
    11761 gattcccgac agcaggtgga ccgcaaagga ccgaacaatg accggctgtc gggagaagcg
    11821 gtcggccagc gctccccaca gcggcaggag cgggatgcca acgagccccg ccagagccgt
    11881 gacccggccg gtccacgcgg gaacctgggc cggagccact ccgaggtttg gcagg
//
LOCUS       DASRZS010000411         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1382280, whole genome shotgun sequence.
ACCESSION   DASRZS010000411 DASRZS010000000
VERSION     DASRZS010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382280"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VFH83_10035"
     CDS             <1..638
                     /locus_tag="VFH83_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6486748.1"
                     /translation="RLFVNSIVVSTSVTLLSLALCGTAAFAFARLRFRGSNALFVLYL
                     STLMIPTSVTLIPSFIVVRILGWLNTYAGLIVPAACAGVGFGTFLLRQFYISIPRELD
                     EAAWMDGAGAWRIFLQIVLPLGKPALVTLGIYTLVTNWNNLLWALVVISKEEMKTLTL
                     ALATMTSAVLYIPPWNQVMAATLIGIVPVLLVFVIAQRYFIQAMTLSGLKG"
     gene            661..1773
                     /locus_tag="VFH83_10040"
     CDS             661..1773
                     /locus_tag="VFH83_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HET6486749.1"
                     /translation="MVDPAQRFLMESGPGPWTVINGRRCLYFGGTGYLGLQTHPEVIR
                     AAQDALERYGTCSATSRTGFGTTPLYQEVERTAARFFGVEDAVYLASGYLANVAGLDA
                     LAQLGRFDAIFLDEGAHWSLGDLVPALRRPVVPFAHRDPDDLAAKLRAHLGAGHRPLV
                     ASDGVFPTFGTIAPVPAYLAAVQPYEGCVWLDDCHGIGVLGPNGRGTSEHFGLTSQRV
                     FFGGTTSKAIGAHGGIIPGTAELVRLIREGHVVSGSNAPSSAAAAAAVTGMTLMMAHP
                     DLRARLRDNALRLKSGLARLGFPQEDSPVPVAAWALPAAADMDRVHAELMRRDIAIQR
                     TRYIGGGADGLLRAVVFATHEPAQIDRLLSELEAVL"
     gene            1920..2228
                     /locus_tag="VFH83_10045"
     CDS             1920..2228
                     /locus_tag="VFH83_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486750.1"
                     /translation="MSRALDEQAGEGREEGVPPLKLPLPPGARNYMTAKGAERLAAEL
                     GGLRWEKRPKLAEAAAESKSGAARRTLLECDRRIDYLAVSMKVLSISYGADSLGGPPV
                     "
     gene            2271..>2714
                     /locus_tag="VFH83_10050"
     CDS             2271..>2714
                     /locus_tag="VFH83_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET6486751.1"
                     /translation="MNISPTVLRASIEVGLDPDETFAIVVGDLRTALGLRGVRWDEGV
                     GSLVTQGAFTVARIVAWEPGRRIACEWHQASWAAGDSEEVELRFDGSPGGTTIVLEHR
                     SRHPWRGGEAELAGWFASAVAAPLIYELTPQGLGSWITDRKARRPS"
BASE COUNT          409 a          906 c          907 g          492 t
ORIGIN      
        1 cgcgtctctt cgtcaactcg attgttgtca gcacttcggt caccctgctc tctctcgccc
       61 tgtgcggcac ggctgctttc gcgtttgcca ggctgcgctt ccgcggaagc aatgccctgt
      121 tcgtccttta cctttccacg ctgatgatcc cgacctcggt cacgctgatc ccgtcgttca
      181 tcgtggttcg gatcctgggt tggctgaata cgtacgccgg gctcatcgtt cccgccgctt
      241 gtgccggagt agggttcggg acctttctcc tgaggcagtt ctacatatcg atcccacggg
      301 agctggacga ggccgcctgg atggatggcg cgggggcctg gcggatcttc cttcagatcg
      361 tgctgccgct cggcaagccc gctctggtga cgctggggat ctataccctg gtcacgaact
      421 ggaacaacct cttgtgggct ctcgtggtga tctccaaaga agaaatgaaa acgctgaccc
      481 tggccctggc caccatgacg tccgccgtcc tctacatccc gccttggaac caggtgatgg
      541 ccgcgacgct gatcggcatc gttcccgtcc ttctggtgtt cgtgatcgcg cagcgatact
      601 tcatccaggc catgacgctc tccggtctca agggataggg gagccgacac gcgcggtccc
      661 gtggtcgatc ccgcccaacg cttcctcatg gagagcgggc cgggcccctg gaccgtgatc
      721 aacggaaggc gctgtctgta cttcggtggc accggctacc tcgggcttca gacgcacccg
      781 gaagtgatcc gtgcggccca ggacgcgctg gagcgatacg ggacctgctc ggcgacctcc
      841 cggaccggat tcggcaccac gccgttgtac caggaggttg aacggaccgc cgctcgcttc
      901 ttcggcgtgg aggacgcagt ctatctggct tcgggctacc tggccaacgt ggctggtttg
      961 gacgccttgg cccagctcgg acggttcgat gcgatcttcc tggacgaagg agcccactgg
     1021 agccttgggg acctggtacc cgctctgcgg aggccggtcg tccctttcgc tcaccgggac
     1081 ccggatgacc tggccgccaa gctgagagct cacctcgggg caggacaccg tccgttggtg
     1141 gccagtgacg gcgtgttccc caccttcggg accatcgcac cggttcccgc gtaccttgcc
     1201 gcggtccaac cgtacgaagg ctgcgtgtgg ctggacgact gccacggcat cggcgtcctc
     1261 gggccgaacg gtcgcgggac ctccgagcat ttcggtctca cttcgcagcg ggtgttcttt
     1321 ggcgggacca ccagcaaggc cataggcgcc cacggcggga tcatcccggg gacggccgag
     1381 ctggtccggc tcatccggga ggggcacgtg gtgagcgggt ccaacgcgcc ctcctcagcc
     1441 gcggccgccg cggcggtcac cgggatgacc ctgatgatgg cccaccccga cctgcgcgcg
     1501 cggctgcggg acaacgcgct tcggctcaag tccggactgg cccggctggg ctttccccag
     1561 gaagactctc cggtaccggt tgcggcctgg gcgctccccg ccgcggccga catggaccgc
     1621 gtccacgcgg agctgatgcg gcgggacatc gcgatccaac gcacccgcta catcggcggc
     1681 ggcgcggacg gtctgctccg cgccgttgtg ttcgccacgc acgagcccgc tcagatcgac
     1741 cggctgctca gcgagctgga ggccgtgctc tagcgaatct ctggccaggg tccggtcggt
     1801 cggccaccct cgtcgccttg ggcgcatccg cggcacgccg cgcggcgacc acccttgagc
     1861 ggccgttgac aggtgttgtt ccccttgtcc gcggagccgc ataagggcac ccttgagcca
     1921 tgagccgagc cctggatgaa caggcgggag aaggacgcga agagggagtt cccccgctga
     1981 aactgcctct cccgccgggg gcccgcaact acatgacggc gaaaggtgcc gaaaggctgg
     2041 ccgcggagct cggggggctc cgctgggaaa agcgccccaa acttgccgag gccgcagccg
     2101 agtcgaagtc cggcgcggcg cggcggacgt tgctggagtg cgatcgcagg atcgactacc
     2161 tcgcggtctc aatgaaagtg ctctcgatct cgtacggcgc tgacagtctt ggtgggcctc
     2221 cggtctgagt gccagaggag gtcgtccggg gtgtcgggcg tggaggagtc atgaacatct
     2281 ctccaacggt ccttcgcgct tccatcgaag tggggctcga cccagacgag accttcgcca
     2341 tcgtcgttgg tgatctgcgt acggctcttg gcctgcgcgg cgtacgatgg gacgaaggcg
     2401 tgggcagcct cgtcactcag ggcgcgttca cggtagcccg tattgtcgcc tgggaacctg
     2461 gcagacggat cgcctgtgaa tggcaccagg cgtcctgggc cgcgggcgat agcgaggagg
     2521 tggaactccg gttcgacggg agccccggtg gaacaaccat tgtcctggaa caccgcagcc
     2581 gccacccctg gagaggcggg gaggcggagc tcgccgggtg gttcgcttcc gccgtcgccg
     2641 cgccgctgat ctacgagctc acaccccagg gactcgggag ctggatcacc gaccggaagg
     2701 cccgaaggcc ttcc
//
LOCUS       DASRZS010000412         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_614950, whole genome shotgun sequence.
ACCESSION   DASRZS010000412 DASRZS010000000
VERSION     DASRZS010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614950"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1243
                     /locus_tag="VFH83_10055"
     CDS             194..1243
                     /locus_tag="VFH83_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486752.1"
                     /translation="MSLGKRVVGIVYAYDPGDDYALLKETGVEWVRLGVPFPWADRMH
                     GAISQEYRDARKEIEAASRAGFHVMPSTAGLGGYVFVASEGRTRWVDSWPEFCGTKGT
                     PAFMESVRAASRWIAQDLGGMVGPLWQVMNEIDIETFRGDYSPQVAADTARASAEGIL
                     SAQSTAMCGTNLANYGPDGLAIMDLVCRAPHPFAYVGDDQYFGSWQGGSVEDWVGVMD
                     GLYDRYGLPVLANEWGYSSAGPLKPVPGSEAVPKGWASVCAVLGWHNELPGGHTEEMA
                     ARYFRRGLEIFATHPHCLGSFMFCWKDARVCYHCGQAGCPAECAWGIVDVNGKPKPAY
                     HAVKEAIGRFYGRTD"
     gene            1245..>2066
                     /locus_tag="VFH83_10060"
     CDS             1245..>2066
                     /locus_tag="VFH83_10060"
                     /inference="COORDINATES: protein motif:HMM:NF028438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HET6486753.1"
                     /translation="MGNADTVVVRQEPLRIPTYPALAAEPNPMFFENRNTQGSRGDVY
                     PHPFTDRLSSAKVDREYAAVVMENEYLRLVLLPELGGRIFSGRDKTNGYDFFYRHAVI
                     KPALIGLCGPWISGGVEFNWPQHHRPGTFDPTSCAVESHPDGSHTVWMGDHEPLNRTK
                     GMVGICLHPGKGYVETKVRLYNRTPLPQTFLWWANAGVHINDRYQVIFPPDVHHAVFH
                     TKNPVTSYPVAKGAFNAGLDYGEGTDVSYWANSPMPTSFFAAESKYAFFGGYDHDA"
BASE COUNT          354 a          653 c          712 g          347 t
ORIGIN      
        1 gcgaacggga gacgcggtac cggtgacggt cgggccccag agcacggccg tggtgaagat
       61 ccggtgcacg ccgcgcgggg tgaccgccgg agaggcgtat tccgtttcgg gcttgctgta
      121 ccgtggcggc gcgcctgtcg accaggttgc cgtcttctac aaaggatccg tctgaagcgg
      181 cgcgggaggg accatgtcac tgggaaagcg ggttgtgggc atcgtctacg cgtatgaccc
      241 cggggatgac tacgcgctcc tcaaggagac cggggtggag tgggtccgcc ttggcgtgcc
      301 atttccgtgg gcggaccgaa tgcacggcgc catctctcag gagtaccggg atgcccgcaa
      361 ggagatcgaa gccgcttccc gggccgggtt ccacgtgatg ccgtccacgg ccgggctggg
      421 cggctacgtg ttcgtggcca gcgaggggcg aacgcggtgg gtggactcct ggccggagtt
      481 ctgcggcacg aagggcaccc ccgcgttcat ggagagcgtt cgagccgcca gccgctggat
      541 cgcccaggac ctgggcggca tggtcgggcc cctgtggcag gtgatgaacg agatcgacat
      601 cgagaccttc cgtggtgact actctccgca agtggcggcg gataccgcgc gagcttccgc
      661 cgaaggaatc ctgagcgctc agagcacggc catgtgcgga accaatctgg ccaactatgg
      721 cccggacgga ctggcgatca tggacctggt ctgccgcgct ccgcatccct tcgcctacgt
      781 gggggacgac cagtacttcg gctcctggca gggggggagc gtggaggact gggtgggcgt
      841 gatggatggc ctgtatgacc gctacgggct gccggtcctg gccaacgagt ggggctactc
      901 ctccgccgga cccctgaagc cggtgcccgg gagcgaggcg gttccgaagg ggtgggcgag
      961 cgtgtgcgcc gtgctgggct ggcacaacga gctgcccggc ggtcacacgg aggagatggc
     1021 cgcgcgatac ttccgcagag gtctcgagat cttcgccacg catccgcact gcctgggctc
     1081 ctttatgttc tgctggaaag atgctcgcgt atgctaccac tgcggtcagg ccggatgccc
     1141 ggcggagtgc gcctggggga tcgtggacgt gaacggcaag cccaaacccg cctaccacgc
     1201 ggtcaaggaa gccatcggcc ggttctacgg caggaccgac tgagatgggg aacgcggata
     1261 ccgtcgttgt ccgccaggag ccgctgagga ttcccaccta cccggccctg gccgcggaac
     1321 ccaaccccat gttcttcgag aaccgcaaca cccagggaag ccgaggtgac gtctatccgc
     1381 atcccttcac ggatcgcctt tcgagcgcca aggtggatcg ggagtacgcc gcggtggtga
     1441 tggagaacga gtacctgcgg ctggtcctgc ttcccgagct gggcgggcgg atcttcagcg
     1501 gccgggacaa gaccaacggc tacgatttct tctaccgcca cgccgtgatc aagcccgccc
     1561 tgatcgggct gtgcggtccc tggatctccg gaggcgtgga gttcaactgg ccgcagcacc
     1621 atcggccggg caccttcgac cccacgagct gcgccgtgga atcccatccg gacggcagcc
     1681 acaccgtgtg gatgggagat cacgagccgc tgaaccggac caagggcatg gtgggtatct
     1741 gcctccatcc gggcaaaggg tatgttgaga ccaaggtccg gctgtacaac cgaacgccgc
     1801 tcccgcagac cttcctctgg tgggcaaacg ccggcgtgca catcaacgat cgctaccagg
     1861 tgatcttccc gccggacgtg catcacgcgg tgtttcacac caagaatccc gtgacgtcgt
     1921 accctgtcgc caaaggggct ttcaacgcgg gcctggacta cggagagggg accgacgtca
     1981 gctactgggc caactctccg atgcccacct ccttctttgc cgcggagtcc aagtacgcct
     2041 tcttcggcgg gtacgatcac gacgcc
//
LOCUS       DASRZS010000413         9518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_998351, whole genome shotgun sequence.
ACCESSION   DASRZS010000413 DASRZS010000000
VERSION     DASRZS010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9518
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998351"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFH83_10065"
     CDS             complement(<1..755)
                     /locus_tag="VFH83_10065"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HET6486754.1"
                     /translation="MKTSWARWVVIVAGIGVYVAVGLYLASHTSIYRLYRSGGTANVR
                     YEKAIVLGIQDEELVTDKEHGGLLTGYQDVSIRLIRGERAGTELRIQNVLNYTSHFLL
                     KKGDTIIVHVDTADADHFTVSVYSVNRVPVVCLLALAFVAALCGIGGSRGFRSLLGMV
                     FTLSSIFLFFIPLLYRGCSPTLAVTVMAAATIAVSLLLLGGLSVKSAAAIAGSFVGIG
                     ISALLAWAAQNMAQISGYTTAEVDSLLAIAGQTG"
     gene            complement(838..2175)
                     /locus_tag="VFH83_10070"
     CDS             complement(838..2175)
                     /locus_tag="VFH83_10070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET6486755.1"
                     /translation="MNPRTRSLRRALVGLTASLFVLLGTAGLSAQAYQPAGLNITPGS
                     DGSSLDFAWLSNGPIGKPVVQIAMASDQKGDAFPAAKATAFTGTASKVAATAFSTDDQ
                     TVTLDSVSNKVTVTGLVDSTDYVYRVGDGKQWSTSSSVSTRDPKSFGFFAVGDPQLGA
                     KSTGPKTLDSDAAGWRTTIEKATTAFSGASFLVSLGDQVNDYNALAAQDAEYAAYFAP
                     AQLRSLPVATVDGNHDFAMGEYYGYHYNLPNLSTRYGISYGNDGDYWFVYGNVLFLML
                     NSNSESVATHDLFIRDAVARNPGARWRVACFHHSVFSEADHYADPDVIDRRTNYPPVF
                     ERYKIDVVLQGHDHAYTRSLPIAKGKPVEGADPAATTIVNPTGIVYFTLNSGSGSKFY
                     DWKDAVPEVFSAARWQGKVPSFSYISVNGNTFSVATFRTDDMSVIDTFTITKD"
     gene            2388..3245
                     /locus_tag="VFH83_10075"
     CDS             2388..3245
                     /locus_tag="VFH83_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6486756.1"
                     /translation="MLPALALVAAFTYAPFVRAVSLSLFIVNRNTFHPTRFVGLSHYL
                     RILNLGGSVFGGMYLRSLLITVWFTLMVAPLSIAAAFGLGLLAQVKLRGIGLFRTVFT
                     STVTISVASASVIWSLIYSPHVGLFKGLMGLLHLQATSLLTDARTALPAVALTTIWTS
                     LGFNFVIALAGLQTIPKDLYESCMIDGAGPWGRFRRVTVPLMGPTLLFLLVITTIACF
                     EAFTQFKVLIDSVGPDQSTNVFVYAIFNAFWMENDYGFASAMSVVLFVVILALTVLQL
                     RLDRGVHYQ"
     gene            3242..4060
                     /locus_tag="VFH83_10080"
     CDS             3242..4060
                     /locus_tag="VFH83_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6486757.1"
                     /translation="MRKVGSATIGLYALLVLFSVVLLFPLLFSLSVSLQGDTVAPRLL
                     PHARLDWGVFTEVFHKEHNLPRWILNSFVVAGLVTLGQVATAALAAYPLAVLRFPGKN
                     LFFLLFLGSLMIPWESTIIPNYLTIASLHWKDSYQGLTVPFLTTGFGIFLLRQSFKSL
                     PTELYEAASVEGCGRTRYLISFLLPLSRPALATLAIYSFVTTWNQYYWPLLVIDNPVW
                     MTGQIGITAFRSSEIASFNLQMAADLVVMAPTLVLLVIGQKQFVRGLTAGALKG"
     gene            4192..5556
                     /locus_tag="VFH83_10085"
     CDS             4192..5556
                     /locus_tag="VFH83_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6486758.1"
                     /translation="MRSLLVITGLSVAAALGFSQTSGVEPLPQPPGSTRVTFWFGLTG
                     VNGDVVRMVVNKYNASQTRYYVDAVSQPDYDATINKLNTSLAGGELPNVVQVYDIGTQ
                     RMIDTGKIVPVQDLIDKERLDIIRDLEPAVASYYTIAGRLYSMPFNSSAPVLYLNRAA
                     FREAGLDPEKRIWTYDEVSTAAKALTRKDSQGNVIRHGIGFTLYSWILEEELATQTAL
                     FADPGNGRQTRAQKLVFNSKEAVAWLSFLKSTVDAGWGESFGIDGGANSTARDAAFVT
                     GEASMTLNSIASLRGYIKSAKDANKGVDVGVAFIPRPAGAKGGVIIGGASLWITSTGT
                     PQQQAGAWDFVKFASTPEIQAFFSSNTGYYPTRRAAYAVQDMKDALALYPQFQVAIDE
                     LRATPASAATQGAVFGTFVKARANIQSAMEQYITGKVPSAAQALDTAARISSAELADY
                     NASY"
     gene            5669..6976
                     /locus_tag="VFH83_10090"
     CDS             5669..6976
                     /locus_tag="VFH83_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6486759.1"
                     /translation="MKTTISMVAAFSVVFALSAPSARGAGLDADPTGAQVTYWYQHSQ
                     DREVAMRRLIDQFNATNPWKIKVTGEYSGPYATIYDKMIAAIAAGNPPELVVAYQNQA
                     AGYEVSKALVDLNPYVNDPKYGISSADLKDFVEGFITQDVSSQFGGMRLGFPPNRSVE
                     VMYYNAAWLNKIGMSDPPQTWDRFYAAAKAATRTEAGTYGYAIDTDASRVFAQVVSRG
                     GGIVSADGKGYVFTGPAMKESMAFMQRLYREGLGRKLAKAGDDQTDFANAMVLFTIGS
                     TSGMPFYTQAVNAGKGGAFDWSVAALPHTTARPTLNLYGASVSIPKTTAQKQLAAWLF
                     IRWFSEPKQQAAWTMVSNYFPVRKSAAALLTDYMAKDRRFAKAWDLLSASDLKAEPPF
                     AGYDLVRDAVSRAYNAILDGADIDSTLVRLEADANKIYRDSAP"
     gene            7036..7734
                     /locus_tag="VFH83_10095"
     CDS             7036..7734
                     /locus_tag="VFH83_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET6486760.1"
                     /translation="MKDVSFAVPTGRITAIMGPSGCGKTTFMRCVNRMHELAPNAHVK
                     GEVLLDGRNVYTQNPILVRQRIGMVFQRPNPFPTMSIYDNVLAGFNLNGTRLPRADRD
                     GIVEGTLRRVTLWDEVKDSLHRRGTFLSGGQQQRLCIARALAMRPEIVLLDEPTSALD
                     PKATAAIEELVVSLKGSVTIVQVTHNIPQAARISDYTAFIHLGVLVEFGPTKEVFTVP
                     KDKRTEEYLSGKFG"
     gene            7743..8405
                     /gene="phoU"
                     /locus_tag="VFH83_10100"
     CDS             7743..8405
                     /gene="phoU"
                     /locus_tag="VFH83_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET6486761.1"
                     /translation="MPVPGIDELVSRLVGYGQFVQAMIEKSRRALVSREPGLPREIID
                     RDEPQANRTEIELEAECVSLIARHQPMARELRTILMVSGITNDLERMADHAVNIAEAV
                     MDHVNDPSLKPEGSILEMFDKTIRMVDDAIRAFANRDPALGQSVCQSDNAVDELASAI
                     LESLTAAIANAPSQVPEKLALLKVAGNLERIADLSTNIGEDVIYMTEGRVIKHHQAEG
                     GR"
     gene            8442..8768
                     /locus_tag="VFH83_10105"
     CDS             8442..8768
                     /locus_tag="VFH83_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486762.1"
                     /translation="MKKHARRSKPTDATRELVVELSALRTRVDEATQQFSLAVKAGID
                     EILHAMTHDGPETAGHVLPEPAAVEKMVIRLRLLADRPLRGRAKDLKRMQDLVTAFGE
                     KVLSQQ"
     gene            8814..9470
                     /locus_tag="VFH83_10110"
     CDS             8814..9470
                     /locus_tag="VFH83_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET6486763.1"
                     /translation="MLHDIDAYPAPSADGSRKRLSELPFIHPGAVVERSRIGSWTEVG
                     RNSRIVESTIGDYTYDDGEVSIVYSEVGRYCSIANRVRINPGNHPMDRVTQHHMTYRR
                     AQFGLAETDDPEFFDWRRDHRCIIGHDVWIGHAAIILAGLRIGTGAVIAAGAVVTRDV
                     EPYQIVAGVPARPKRKRFEDAVIRGLLRSEWWEWSREELVDRWRDLCSVSGFLEKYGH
                     "
BASE COUNT         1766 a         3055 c         3011 g         1686 t
ORIGIN      
        1 cccgtctgcc ccgcgatggc cagaagggag tccacctccg ccgtggtgta ccccgagatc
       61 tgcgccatgt tctgagccgc ccaggcaaga agggctgaga tcccgatgcc gacaaaggag
      121 ccggcgatcg cggcggcgct cttcacggag agaccgccga ggaggaggag ggagacggcg
      181 atcgttgccg ccgccatgac ggtcacggcc aatgtcgggg aacaaccgcg gtaaagcagc
      241 gggatgaaga acaagaagat gctcgacagt gtaaagacca tgccgagcag agaacgaaat
      301 ccccgcgagc ctccgatgcc gcataaagcc gccacgaagg ccagggcgag caggcacacc
      361 acgggcacgc ggttcacact atacacggag acggtgaagt ggtcggcgtc ggcggtatcc
      421 acgtggacga tgattgtgtc gcccttcttg aggaggaaat gactcgtgta gttgagaacg
      481 ttctgtatcc tcagttccgt tccagcgcgc tcgccacgga tcaagcgtat ggaaacatcc
      541 tggtaccccg tgagcaggcc gccgtgctcc ttatccgtta ccaactcctc atcctggatg
      601 cccaggacaa tcgctttctc atagcggacg ttggccgttc ctccggaccg gtagagccgg
      661 tagatggaag tgtgggacgc gagatagagc ccgacggcga cgtagacgcc gatccccgcg
      721 acgatgacga cccatcgcgc ccagctcgtt ttcactgctc cctccggcga tcgaggaaag
      781 gaagggtccg tccaggccac cgttgggcgt ccggacggac cgcagaatgc cgctggatca
      841 atcctttgtg attgtgaagg tgtcgatcac cgacatgtcg tccgtgcgga aggtcgccac
      901 ggagaaggta tttccgttga cggagatgta gctgaaggag ggaaccttcc cctgccagcg
      961 cgccgcgctg aatacctcgg gaaccgcgtc cttccagtcg tagaacttgc tgcccgaccc
     1021 ggagttgagc gtgaagtaga caatgcccgt cgggttgacg atcgtggttg ccgcgggatc
     1081 ggccccttcg acgggcttgc cctttgcgat gggcaggctc cgggtatagg cgtggtcgtg
     1141 tccctggagg acaacgtcga tcttgtagcg ctcgaagacc ggaggatagt tggtgcggcg
     1201 gtcgatgaca tcagggtccg cgtagtggtc agcctcgctg aacacggagt ggtggaagca
     1261 ggccacccgc caccgggcgc cgggattccg ggcgaccgcg tcgcgaatga acagatcgtg
     1321 cgttgccacg ctctccgagt tgctgttcag catgagaaag agcacgttgc cgtaaacaaa
     1381 ccagtagtcg ccgtcgttgc cgtacgagat tccgtaccgg gtggaaaggt tgggcaggtt
     1441 atagtgatag ccgtagtact cgcccatcgc aaagtcgtgg ttgccgtcga cggtggctac
     1501 gggaagggaa cgcagctggg ccggggcgaa gtaggcggca tactcggcat cctgggcggc
     1561 aagggcgttg tagtcgttga cctggtcccc gagagaaacc aggaagctcg cgccggagaa
     1621 ggccgtggtt gccttctcta tcgtggttcg ccagcccgct gcatcggaat cgagcgtctt
     1681 gggaccggtc gacttggcgc caagctgcgg atctccgacg gcgaagaaac cgaagctctt
     1741 ggggtcgcgc gtgctgaccg acgatgacgt gctccactgc ttgccgtctc caacccgata
     1801 gacgtagtcc gtagagtcca cgaggccggt gacggtcacc ttgttggata ccgaatcgag
     1861 ggtcaccgtc tggtcgtcgg tgctgaatgc cgtggccgcg accttgctcg cggtccccgt
     1921 gaacgccgtt gccttcgcgg cgggaaacgc gtcgcctttc tgatccgacg ccatggcgat
     1981 ctgcacgaca ggcttgccga tcgggccgtt cgacaaccat gcgaagtcga gcgagctacc
     2041 gtcggagccc ggcgtgatgt tgagccccgc aggctggtaa gcctgcgcgg agagccccgc
     2101 cgtgccgagc agcacgaaca gggatgccgt gaggccgacc aacgcccggc gcaaagaccg
     2161 agttctggga ttcatcgtgc acctcctgat caaagagtga tggaacgatc ggagcctacc
     2221 ggtgccgtgt taagattccg tggtgagtcc agggaatcct gacggcgccg aaagctaacc
     2281 cgttcctaac agagcgcgcg taggctgcct cgatgtccgg tatgtcgggt ggaccgagcg
     2341 ggggaccggg taactcctgg cggaacctgg gtccagccct cctgtacatg ctcccagccc
     2401 ttgccctggt ggccgccttc acctacgccc cgttcgtccg cgccgtctcc ttgagcctct
     2461 tcatcgtcaa ccgcaacacg ttccatccca cgcgtttcgt ggggctctcc cactacctgc
     2521 ggatcttgaa cctcggcggc tccgtgttcg gagggatgta ccttcggagc ctcctgatca
     2581 ccgtctggtt caccctcatg gtggcgccgc ttagcatcgc ggcggcgttc ggtctgggtc
     2641 ttctggcgca ggtgaagctt cgggggatcg gcctgttccg caccgtgttc acctccaccg
     2701 tcaccatctc cgttgccagc gcgagcgtga tctggtccct gatctacagc ccgcacgtcg
     2761 gcttgttcaa gggcctgatg ggcctcctcc atctgcaggc gacgagcctc ctcacggacg
     2821 cgcgtacggc gctccctgcg gttgccctga ccaccatctg gaccagcctt ggcttcaact
     2881 tcgtcatcgc cctggcgggt ctccagacca tccccaagga cctctatgag agctgcatga
     2941 tcgacggcgc cggaccctgg ggacgcttcc gacgcgtgac cgttcccctg atgggcccga
     3001 ccctcctgtt cctgctggtc atcacgacca tcgcctgctt cgaggccttc acgcagttca
     3061 aggtcctcat cgacagcgtg ggaccggatc aatccaccaa cgtattcgtc tatgcgatct
     3121 tcaacgcctt ctggatggaa aacgactacg ggtttgcgtc cgccatgtcc gtggttctct
     3181 tcgttgtcat cctggcgttg acggtcctcc agctccgcct ggatcgcggg gtgcactacc
     3241 agtgagaaag gtcggttccg ccaccatcgg cctgtatgcg ctcctggtcc tcttctcagt
     3301 cgtgctcctt ttcccgctgc tcttcagcct gtccgtgtcc ctccagggag acacggtggc
     3361 gccgcggctc ctcccccacg ctcggctgga ctggggggta ttcaccgagg tgttccacaa
     3421 ggagcacaac ctcccccggt ggatcctcaa ctccttcgtg gtcgcgggcc tcgtcacgct
     3481 gggccaggtg gccaccgccg cgttggccgc ctacccgctg gcggtcctgc ggttcccggg
     3541 caagaacctg ttcttcctgc tcttcctggg aagcctcatg atcccctggg aatccaccat
     3601 catcccgaac tacctcacga tcgcctccct gcactggaag gacagttacc aggggcttac
     3661 ggtccccttc ctgaccaccg ggttcggcat cttcctgctg cggcagagct tcaaatccct
     3721 gccgaccgag ctctatgagg cggcctccgt ggagggctgc ggccgcacgc gttatctgat
     3781 ctccttcctt ctgcccctct cccggccggc tctggccacc ctggccatct actcgtttgt
     3841 cacgacgtgg aaccagtact actggccgct tctggtcatc gacaacccgg tatggatgac
     3901 cggccagatc ggcattaccg cctttcgatc gtcggagatc gcttcgttca acctgcagat
     3961 ggcagcggac ctggtcgtca tggcgccaac cctggtgctt ctggtgatcg ggcagaagca
     4021 gttcgttcgg ggactgacgg cgggggcgct gaagggttag gcgccacgcg ggccaaccgg
     4081 agagggccgg ccccggccga ataaacggtt tcgtaacggt cccctcacca aaggctaacg
     4141 aacccggaag atcctgcctc caatatctgc aaggaggatg gtgtgaaagg gatgagatct
     4201 ctgctggtaa tcacgggtct ttccgttgcc gcggcgcttg gattctccca gacttccggg
     4261 gtcgagccgc tgccgcagcc gccaggttcc acgcgggtca cgttctggtt cggcctcacg
     4321 ggggtgaatg gggacgtggt ccggatggtg gtgaacaagt acaacgccag ccagacccgg
     4381 tactatgtcg atgcggtttc ccagccggat tacgatgcca ccatcaacaa gctcaacacg
     4441 agcctggcgg gcggagagct tcccaacgtg gtacaggtgt acgatatcgg gacccagcga
     4501 atgatcgaca cggggaagat cgtgcccgtg caggacctga tcgacaagga gcggctggac
     4561 atcatccggg acctggagcc ggcggtcgcc agctactata ccatcgccgg ccgcctctac
     4621 tccatgccgt tcaacagctc agcaccggtc ctgtacctca accgtgcggc tttccgcgag
     4681 gcaggcctgg atcccgagaa acggatctgg acctatgacg aggtcagcac cgcggccaag
     4741 gccctgaccc gaaaagactc gcaggggaac gtcatccggc acggcatcgg ctttacgctg
     4801 tatagctgga tcctcgagga ggagcttgcc acccagacgg ccctcttcgc ggaccccggc
     4861 aatggtcgcc agacccgggc ccagaagctc gtcttcaatt ccaaggaggc cgtagcgtgg
     4921 ctgagcttcc tgaagtcgac ggtggatgcg ggatggggtg agagcttcgg gatcgacggc
     4981 ggggccaaca gcaccgcgcg agacgcggcg ttcgtcacgg gtgaggccag catgaccctc
     5041 aacagcatcg cctcgcttcg aggctacatc aagagcgcaa aggacgccaa caagggcgtg
     5101 gatgtcggcg tggcgttcat cccccgaccg gccggcgcca agggcggcgt catcatcggc
     5161 ggcgcctctc tttggatcac gagcaccggg actccccagc agcaggcggg cgcatgggac
     5221 ttcgtgaagt tcgcgtcgac gccggagatc caggcattct tctcctccaa taccggctac
     5281 taccccaccc gcagggccgc ctatgcggtt caggacatga aagacgcgct ggcgctctat
     5341 ccacagttcc aggtggcgat cgacgagctg cgggcaaccc ccgcctccgc cgccacacaa
     5401 ggggcggtct tcgggacctt cgtcaaggcc cgggccaaca tccagagcgc catggagcag
     5461 tacatcaccg gcaaggttcc ttccgctgcc caggcgctcg acacggccgc caggatctct
     5521 tccgccgagc ttgccgacta caacgcctcg tattgagact attcatgcta tcttaaccgc
     5581 aagctcaaca tcccttaacg gttcctgagg atcttgcggc tgtggtctcc cgacagggca
     5641 gagaggccac cggaggacac tgacgctcat gaagacgacg atctccatgg ttgcggcctt
     5701 ctcggtcgtg tttgcgctgt ccgctccgtc cgcgcgaggc gccggcctgg acgcggatcc
     5761 gaccggcgcc caggtgacgt actggtacca gcactcccag gaccgggagg tcgccatgcg
     5821 gaggctgatc gaccagttca acgcgaccaa cccgtggaaa atcaaggtca cgggcgagta
     5881 ttcgggaccg tacgccacca tctacgacaa gatgatcgcg gccatcgccg ccggaaaccc
     5941 gccggagctg gtcgtggcct accagaacca ggccgccggc tacgaggtca gcaaggccct
     6001 ggtggatctg aacccgtacg ttaacgaccc gaagtacggt atctcctcgg ccgatctcaa
     6061 ggacttcgtg gagggcttca tcacccagga cgtaagcagc cagttcggcg ggatgcggct
     6121 ggggtttccg cccaaccggt ccgtggaggt gatgtactac aatgccgcgt ggctcaacaa
     6181 gatcgggatg agcgatccac cgcagacgtg ggaccggttc tacgccgcgg ccaaggcggc
     6241 gaccaggacc gaggccggca cgtatggata cgccatcgac acggacgcct cccgggtctt
     6301 cgcccaggtc gtctcccggg gcggagggat cgtctccgcg gacggcaagg gatacgtctt
     6361 caccggtccg gcaatgaagg aaagcatggc gttcatgcag cggctctacc gggaagggct
     6421 gggccgcaag ctggccaaag ccggcgacga ccagaccgac ttcgccaacg caatggtgct
     6481 cttcaccatc ggctcgacct cgggaatgcc cttctacacc caggcggtga acgccggtaa
     6541 aggcggggcg ttcgactggt ccgtcgcggc cctgcctcac accacggcac gaccgactct
     6601 gaatctctac ggagccagtg tgagcatccc caagaccacg gcgcagaaac agcttgccgc
     6661 ctggctcttc atccgatggt tctccgagcc aaagcagcag gccgcctgga ccatggtctc
     6721 caactacttc ccggtgcgga agtcggcggc cgccctgctg acggactaca tggcgaagga
     6781 tcgacggttc gccaaggcct gggatctcct cagcgccagc gacctgaaag cggagccacc
     6841 gttcgccgga tacgacctgg tgcgggatgc ggtgagccgg gcctacaacg cgatcctgga
     6901 cggggcggac atcgactcga ccctggtcag gttggaggcg gacgcgaaca agatctatcg
     6961 agactccgcg ccgtgagctc agagacgatc ctggaggcgc ggcacctcgt cgccggcttc
     7021 gacggcaagg acgtcgtgaa ggacgtgtcc ttcgccgtcc cgacggggag gatcacggcc
     7081 atcatggggc cgtccggctg cggcaagacg acgttcatgc gctgcgtcaa ccgcatgcat
     7141 gagctggcgc ccaacgccca cgtaaagggc gaggtcctcc tggatgggag gaacgtttac
     7201 acgcagaacc ccatcctggt ccgccagcgg atcggaatgg tcttccaacg gcccaacccg
     7261 ttcccgacca tgagcatcta cgacaacgtg cttgcgggtt tcaacctcaa cggcactcgc
     7321 ctgccgaggg ccgatcggga cgggatcgtg gaagggaccc tgcgtcgggt gacgctctgg
     7381 gacgaggtaa aggactctct ccaccgtcgg ggcaccttcc tctccggtgg acagcagcag
     7441 cgactgtgca tcgcccgggc gctggccatg cggcccgaga tcgtgcttct ggacgagccg
     7501 acctcggcgt tggaccccaa ggccaccgcc gccatcgagg agctggtagt gtccctgaag
     7561 ggatcggtca ccatcgttca ggtgacccac aatatcccgc aggcggcgcg aatctccgac
     7621 tacacggcgt tcatccacct tggggtgctc gtggagttcg gaccgaccaa agaagtcttc
     7681 accgtcccca aggacaaaag gaccgaagag tatctctccg gcaagttcgg ataggagtga
     7741 ccatgccggt accgggaatt gacgagcttg tttcccgact cgtcgggtat ggccagttcg
     7801 tacaggcaat gatcgagaag agccgccgcg ccctcgtctc ccgggagccc ggactgccgc
     7861 gggagatcat cgaccgggat gagccccagg ccaaccggac ggagatcgag ctggaggcgg
     7921 aatgcgtctc cctcatcgcc cgccaccagc ccatggcgcg ggagctgcgg accatcctca
     7981 tggtgagcgg gatcaccaac gacctggaac gcatggcgga tcacgcggtg aacatcgccg
     8041 aggccgtgat ggatcacgtg aacgacccct ccctcaagcc ggaagggtcc atcctggaga
     8101 tgttcgacaa gaccatccgc atggtcgatg acgcgatccg cgcctttgcc aatcgggatc
     8161 cggccctggg ccagagcgtc tgccagtcgg acaacgccgt ggacgagctg gccagcgcca
     8221 tcctggagag cctgacggcc gccatcgcca acgccccctc ccaggtgccc gaaaagctgg
     8281 cgctgttgaa ggtcgccggg aatctcgaga ggatcgctga cctctccacg aacatcggcg
     8341 aggatgtgat ctacatgacc gaaggccggg tgatcaagca ccatcaggcg gagggcggcc
     8401 gatagagtcc ggccccctcc ctcacgccag cggagggcag catgaagaag catgcaagac
     8461 ggtcgaagcc caccgacgcc actcgggagc tcgtcgtgga gctctcggcg ctgcgcacga
     8521 gggtcgacga ggctacacag cagttctctc tggcggtgaa ggcaggcatc gacgagatcc
     8581 tccacgccat gacccacgat ggacccgaga ccgcgggaca cgtcctcccc gagccggccg
     8641 ccgtggagaa gatggtgatc cgcctccgtc tcctggccga ccgtcctctc cgcggacgcg
     8701 caaaggacct caagaggatg caggacctcg tgacggcgtt tggcgagaag gtcctgagcc
     8761 agcaatagcc ggcggccgtt gccgttgccc agggtccgtt tccggaggtt tccatgctgc
     8821 acgacatcga tgcttacccg gcacccagcg cagacggctc gcggaaacgc ctgtccgagc
     8881 ttccgttcat ccaccccggt gcggtcgtcg agaggagccg gattggctcg tggacggagg
     8941 tcggccgcaa tagcaggatc gtggagtcga ccatcgggga ctacacctat gacgatggcg
     9001 aggtgtccat cgtgtattcg gaggtcggca ggtactgctc catagccaac cgggtccgga
     9061 tcaacccggg caaccacccg atggatcggg tgacgcagca tcacatgacc taccgaaggg
     9121 ctcagttcgg cctcgctgag acggacgacc cggagttctt cgattggagg cgtgaccatc
     9181 gatgcatcat cgggcacgat gtctggatcg gacacgccgc tatcatcctt gcgggactgc
     9241 ggatcggcac gggcgccgtg atcgcagcag gggccgtcgt gacccgggac gtggagccat
     9301 accagatcgt ggcgggggtg ccggcccgtc ccaaacggaa acggttcgag gacgcggtga
     9361 tccgcgggct tcttcgcagc gaatggtggg agtggagccg tgaggagctc gtggaccgct
     9421 ggagagacct gtgctccgtc tccgggttcc tggaaaagta cgggcactga tgctcgacgg
     9481 tcctgcgccg aggttagcct cgagccagcg attgggcg
//
LOCUS       DASRZS010000414         2691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2919474, whole genome shotgun sequence.
ACCESSION   DASRZS010000414 DASRZS010000000
VERSION     DASRZS010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2919474"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="VFH83_10115"
     CDS             complement(<1..1269)
                     /locus_tag="VFH83_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6486764.1"
                     /translation="MTLRARLTLSYVFIALLCVLMVSALANGVLESSFRRYLENGLRS
                     QSMQVASEVGTQVSADGVWDQQGISLIGMGALEHGLIVKVATADGTVIWDATVHNNGL
                     CQQMLLHMAANMASRYRNWHGVYTEASYPVRTSFRQIGAVTVGYYGPFFLNDEDLSFI
                     TALNRLLLWIGLAALVIAVGFGLLMARRITVPLARVVSATQRIAAGSRTVRITEKTRV
                     RELDGIASAVNDLSRSLTEQEQLRRRLTADMAHELRTPLATLQSHLEAMIDGVWQPEP
                     ERLGGLHDEILRINRLVTDLENLARYEGRSLSLRRAEIDVADLVQGIVRNHAAQFRAK
                     GVALEFAAEGPLRDQADPDKLSQAVINLLSNALKFTPTGGRVDVRVSGSDSEVRIEVA
                     DTGIGISAEDLPRVFERLYRVDESRSRATGG"
     gene            complement(1266..1976)
                     /locus_tag="VFH83_10120"
     CDS             complement(1266..1976)
                     /locus_tag="VFH83_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6486765.1"
                     /translation="MTPPRKRVLVVDDELPIAEVVGTYLRLAGYEPLCVASGSEAMEV
                     FETARPSLVILDLMLPDLSGEEVCRRLRARSRVPIIMLTAKVEEEDAQRGLDLGADDY
                     VTKPFSPRLLMARVEAVLRRASSDSLPLAGHLRFAEGELEIDMGLAEVRRHGNPISLT
                     PREFRLLRVLASNPGRIFSREELINRALGDDFDGYDRTIDAHIKNLRQKVEADPRQPR
                     FIHTVHGLGYRFSSERGA"
     gene            complement(1978..2184)
                     /locus_tag="VFH83_10125"
     CDS             complement(1978..2184)
                     /locus_tag="VFH83_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014097626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HET6486766.1"
                     /translation="MTKQILSVDGMHCEHCVRAVKTSVSALAGVSQVEVSLAENQVTV
                     GFDPAKTDLASIKGAIEEQGYTVR"
     gene            complement(2266..>2691)
                     /locus_tag="VFH83_10130"
     CDS             complement(2266..>2691)
                     /locus_tag="VFH83_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal transporter"
                     /protein_id="HET6486767.1"
                     /translation="GAIPSGFVGGAIPTDAIGIAPVRGDSQVVDIAVDQAGYTPAVVV
                     LQRGLKAKIRFTPRNLTTCNSVVTFPEYQGSLCLQNGELETPYLEVTQDFGFQCGMGM
                     LHGYAKVVDDIRHVDLNQVRKTVAAYRPASIGGASCCGR"
BASE COUNT          433 a          915 c          879 g          464 t
ORIGIN      
        1 tcctcccgtg gctcggctcc tggattcgtc cacccggtag agccgttcga atacccttgg
       61 caggtcctcc gcgctgatcc cgatgcccgt gtcggccacc tcgattcgga cttcggagtc
      121 ggagccgctc acgcgtacgt ccacccgacc tcccgtcggc gtgaacttca gcgcgttgga
      181 gagcaggttg atgaccgcct ggctcagctt gtccgggtca gcctggtcgc gaagcggtcc
      241 ctcggcggcg aactccagcg caacgccctt ggcccggaat tgggcagcgt gattgcgcac
      301 gatcccctgc accagatccg cgacgtcgat ctctgcccgt cgcaggctca gggacctgcc
      361 ttcgtaccgc gccaggttct ccaggtccgt caccaggcgg ttgatccgca ggatctcgtc
      421 gtgaagcccg cccaggcgct ccggttccgg ctgccacacg ccgtcgatca tggcttccag
      481 gtggctctgg agcgtggcaa gcggcgtgcg cagctcgtgg gccatatccg ccgtgagcct
      541 gcggcggagc tgctcctgct ccgtgaggga gcgggacagg tcgttgacgg cggaggcgat
      601 gccgtccagc tcccgtaccc gcgtcttctc ggtgatgcgc acggtcctgc tgccggccgc
      661 gatgcgctgc gtggcgctca ccacgcgggc gagcgggacg gtgattcgtc gggccatgag
      721 aagcccgaac ccgacggcga tgaccagggc cgccagaccg atccagagga ggagccggtt
      781 cagcgcggtg atgaaagaaa ggtcctcgtc gttgaggaaa aacggaccgt agtatcccac
      841 cgtgacggcg ccgatctggc ggaagctggt ccggaccggg tagctcgcct ccgtgtagac
      901 gccgtgccag ttgcggtacc ggctggccat gttggccgcc atgtgcaaga gcatctgctg
      961 gcaaagcccg ttgttgtgga ccgtggcgtc ccagatcacg gttccgtccg cggtggccac
     1021 cttcacgatc agcccgtgct ccagcgcgcc catcccgatg agggagatcc cctgctggtc
     1081 ccacaccccg tcggcggaga cctgggtccc gacctccgag gccacctgca tgctctggga
     1141 ccgcagcccg ttctccagat agcggcggaa gctgctctcc aggacgccgt tagccagcgc
     1201 gctcaccatc aggacgcaca ggagggcgat gaatacgtag gaaagggtga ggcgggcccg
     1261 gagcgtcatg cgccacgctc cgagctgaac cggtacccca gaccgtggac cgtgtggatg
     1321 aaccgaggct gacgagggtc tgcttcgacc ttctggcgga ggttcttgat gtgggcgtcg
     1381 atggtccggt cgtagccgtc gaagtcgtct cccaaggctc ggttgatgag ctcctcccgg
     1441 gagaagatcc gcccggggtt ggaggccagg acccgtagga gccgaaactc ccggggcgtc
     1501 aggctgatgg ggttgccgtg ccggcgcacc tccgccaggc ccatgtcgat ctccagctcc
     1561 ccttcggcga accgcaggtg accggccagg ggaagggaat ccgaggaggc gcgtctcagg
     1621 accgcctcaa cgcgggccat gaggagacgc gggctgaacg gcttggtgac gtagtcatcc
     1681 gcgccgaggt ccaggccccg ctgcgcgtcc tcttcctcca ccttcgcggt gagcatgatg
     1741 atggggactc gcgagcgcgc gcgcagccgt cggcagacct cctcccccga gaggtccggc
     1801 agcatgagat ccaggatcac cagggagggg cgggcagtct cgaacacctc catcgcctcg
     1861 ctcccgcttg cgacgcacag aggctcgtag ccggccagac gaaggtaggt cccgaccacc
     1921 tccgcaatcg gaagctcgtc gtcgacgacc agaaccctct ttcggggcgg ggtcatttca
     1981 gcggaccgtg tatccctgct cctcgatcgc gcccttgatg ctcgcgagat cggtcttggc
     2041 cgggtcgaac cctaccgtga cctgattctc cgctaggctt acctccacct ggctcacccc
     2101 cgccagggcg cttacggaag ttttcacggc acgcacgcag tgctcacagt gcataccgtc
     2161 aacgctgagg atctgcttgg tcatggattg tctcctgcga tcaaatatgc tccagaaccg
     2221 cctggggcgt ccacgccgcg cggcggctcc gacgcgccgt ggcggctacc ggccgcagca
     2281 ggacgcgcca ccgatggagg cgggacggta agccgccacg gtctttcgga cctggttcag
     2341 gtccacgtgc ctgatgtcat ccaccacctt cgcgtacccg tgcagcatcc ccatgccgca
     2401 ctggaatccg aagtcctggg tcacttccag gtagggagtc tccagctcgc cattctgcag
     2461 acacagcgaa ccctggtact ccggaaaggt caccacggag ttgcatgtgg tgaggttccg
     2521 cggggtaaac cggatcttcg ccttgaggcc acgctggagc accaccacgg cgggcgtgta
     2581 gccggcctgg tctaccgcga tgtccaccac ctgggagtct cccctcaccg gggcgatgcc
     2641 gatggcatcc gtcggaatgg cgccgccgac gaaccctgag gggatcgcgc c
//
LOCUS       DASRZS010000415        11963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1382938, whole genome shotgun sequence.
ACCESSION   DASRZS010000415 DASRZS010000000
VERSION     DASRZS010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11963
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382938"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFH83_10135"
     CDS             <1..487
                     /locus_tag="VFH83_10135"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6486768.1"
                     /translation="DLYFRLETHHVHIPPLRDRLKDLPSLIDHFVSQASAELNRPAPA
                     VPADVYLLLKSHPFPGNVRELRSLIFDAVSRQGPGSGEAFPVRFLKTALGHRSTRMPV
                     STGDDYFLGALQLPTIKEATEKLVAEALKRADGNQSVAARILGITPQALSQRLRRAGD
                     R"
     gene            complement(554..1648)
                     /locus_tag="VFH83_10140"
     CDS             complement(554..1648)
                     /locus_tag="VFH83_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6486769.1"
                     /translation="MKSTQTDIVSGLGLTMPPLEPRVLVVDDDTILLRSLRLAIRSRG
                     LVCDTAETLAAARQALTRERYGILFLDLALPDGTGTSLLDEPGRVSESSVVVIITAEQ
                     GMETVVRVVRKGAYDFICKPFGLELFEERLEKSVQEWRYRHRARSYQDDIDGLISSMT
                     DELKRAESRIDEIYDMTVNALGAALDLRDPETEEHCQRVAANAVTLGKVMGVLPSDLR
                     NLKWGAYLHDIGKIGIPEQVLSKPGPLTPQEMDLVRVHPVLGHRLILRIDFLRDAAEV
                     VLCHHERYDGTGYPQGLRGEAIPLHARIFALIDAQDAMLYDRPYRKALTRRYLEEELR
                     SQAGRHFDPRVVEAFLRIPEQAWTVNPDAS"
     gene            complement(1645..2148)
                     /locus_tag="VFH83_10145"
     CDS             complement(1645..2148)
                     /locus_tag="VFH83_10145"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HET6486770.1"
                     /translation="MERTVRLVQMAEMAVVENSHLLKTTLGSCVGIVLYDEMRRVTGL
                     AHIMLPRTLERDTSVGKYADSALPALLRRILQRGGSRERITARIAGGANMFRAATDRG
                     IATIGEQNIAAVKGLLAELKIPVVFEDVGGEHGRTVVFDNETGRMEVKTLRKVPTPEP
                     SKEGRES"
     gene            complement(2523..2891)
                     /locus_tag="VFH83_10150"
     CDS             complement(2523..2891)
                     /locus_tag="VFH83_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004773967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET6486771.1"
                     /translation="MKKGSGPGSIDEYILACPAPVQKRLQTIRRLVRRLAPDATEKIS
                     YRMPTFYLNGNLVYFAAHAKHIGFYPGTGAMASFKLQLAPYQKGKGTLQFPMDEPLPV
                     RLIEDLVRIRVQQNRAKRPR"
     gene            complement(2925..4064)
                     /locus_tag="VFH83_10155"
     CDS             complement(2925..4064)
                     /locus_tag="VFH83_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET6486772.1"
                     /translation="MLTQHRLRHLGMAPLLALSAIGAGAESAGAAGSSASSAAAPLPA
                     LHVEGKWIRDASGHAVTLRGVSVADLDAIHNGDRDQSGPTTVFDIIDRGCSSGWDIDV
                     FRLPVHPEVTDETGSHGWLHYAPQDYFERILDPAVQHAIRRGKYVIVDWHYIGADWRD
                     AAVESSTEAFWLGKGAWPGVAVRYANQPSVLFELFNEPGGGSWSDWKVTAQRWIAGIR
                     ARGAGNLVIVGGPVWSQVMPQRPSDLLSGPNIVYASHVYPAHVHGAMPPWIDFVSSVA
                     PVMMTEWGFEKDGPMPVNGTASSYGRIFRTYIDAHPNVGWTAWCFDSVYRPVMFDTAW
                     VLLGNGKSTHTSRFGGGAEDTAENYMGQFVKDWLAEAKAKRPPGP"
     gene            4214..5842
                     /locus_tag="VFH83_10160"
     CDS             4214..5842
                     /locus_tag="VFH83_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486773.1"
                     /translation="MSLAIVLAAAVWVCAPGWALVTADPNDAVYRDLDRWQQQGYLEP
                     LPSLRPYPTQLIVSALNEVAAKAGPVDAELARSYLDALRGGLLGIPVHASATYLREQK
                     QADGLNRLGLQADGSGTIGDLVGFSVRGRLVADDSTAEVFPRWADDYEATLATQTPNT
                     GTRSSLPISFGGQPFAGYGVLNGLVSFGTDRLWIQGGLVQSSFGPVLESPILGPQAPQ
                     AGHFSATYRGSWFTYTNLVEELVAYYAVDASGNTSLITSSSGSPYPQKYLFLQTFNLS
                     PWPWVQADIVQAVVSGGRFSPTYLLPLLPLYTQAYLGDWDNSFAGGYFQFNLPFGIRP
                     SLLLYVDDLDLKKLLQLNFNTDGNKVAIQAGLSWAPPLSVPVQASLTYTAITPYTYTH
                     FSYDTLNYLQYTNLGQQLGSILPPNSDQWTLRVEVIPLSWLRLDLWGKLIRHGNGSDY
                     GSGVVVGTGTVYDDGALTGPITFLNQNSFLTQAVLEKDEQIGIEGTADLAYIGLRGEL
                     SLSYTLEGVQNRDLNPAAPNELNNILGIRLSFGF"
     gene            complement(5847..6839)
                     /locus_tag="VFH83_10165"
     CDS             complement(5847..6839)
                     /locus_tag="VFH83_10165"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET6486774.1"
                     /translation="MSLGPSVPRGVDLLVFSPHLDDAACSCGGRIAAAVRAGARVVVV
                     TPFAGSPAAEGRRAGGTLSPFASMSSRREEDRRAMDRLGVVPLWMDIEEAIGRSPRYR
                     SFLALFGRIRRSDTALLVELQADLERLCRASPGAELVFPLGVGGHVDHRILFEVSRRM
                     MRAPAVAGRVSYFEEIPYSFVPSMVEHRLAQVKARVASPNLPADLAARESILRVSRRA
                     LEGILTVPVIRDSVRPPQDAILRVLLMARIAGQRARGGSGRSSRGGVLELRPDPVDVT
                     DCASVKAQAIACYGSQLPALFGDAAAWRRAALTYSSLLGAGPGRLIERYWRPAT"
     gene            complement(6836..7549)
                     /locus_tag="VFH83_10170"
     CDS             complement(6836..7549)
                     /locus_tag="VFH83_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6486775.1"
                     /translation="MPDPTAPRRRPTAIVRPYRREDREAVRDIFRAGGQRGDPVSNYI
                     EEEDIPLALFVDYHLDHEPGCCFVAEAEGKVVGYIAGCLDNDRYNWVVLTRYIPRLLV
                     RVLWRLFTLQYHNTSTFRVILWFMTRSWREIPEPPRGRYPAHLHIALAPEYRGLRLGR
                     QMVDTAVDRIKSLGAVGAHGVVIEEAGHNAFAAVMAGTLLESRPCTLWAHCSDKRWMF
                     RLLVRDLRDPSSRAAAGRT"
     gene            complement(7542..8309)
                     /locus_tag="VFH83_10175"
     CDS             complement(7542..8309)
                     /locus_tag="VFH83_10175"
                     /inference="COORDINATES: protein motif:HMM:NF016377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6486776.1"
                     /translation="MAIPRVLHQIWLQGEREVPERYDDLRRSWKERNPGWEHRFWDDA
                     SIRALVAGRYPWFLSVYDGYRYLHQRVDSGRYFILYEQGGVYADLDTECLRPMDRLLR
                     QYPRAGLLVSEQPFGPIEMKLIRLVVGARRIVTNAVMAAAPQAPPLAETIRRLPHAVR
                     RLGFLRELNITYSTGPALLSQALDRWSSSDPSVAVVPAACFEPHFGFDLGEHAESTEV
                     ERYVVHSQEATWHAPFLKDVFESYFKLKNLLRLADHA"
     gene            complement(8309..9484)
                     /locus_tag="VFH83_10180"
     CDS             complement(8309..9484)
                     /locus_tag="VFH83_10180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6486777.1"
                     /translation="MRIALFTDSNIHYSDGVARIVQELARHVERNPGHALLICHRSRE
                     GIQRNGSSVETVGVYRAALTIPGYNVYPWLYLRSPKRALLREVRSFQPQVLLTVTPYI
                     LRGIGSTAFFLSRRLRVPVVGSFDVQLAKLAEYYLANIFRFALVIRFFQWLIDRRVGL
                     FRQCTRILVPSQSVERFVQQSWPAIPTVLFRRAVDAEVFHPRFRSEEFRSRHGLSGKE
                     VILFVGRLAREKNLPELARMYGRIKQRRPSAALLIVGEGPEREAVEAMGLPDVLFTGR
                     LTGIELSTAYASSDVFAFASLAEAGPMVILEAMASGLPVVVARTGGARDDVTDGENGF
                     VAPDLPAFEAGLERLISDCALRSRMGEAARRHAEAHGWDTVWGAVLSALAVAPRGRG"
     gene            9625..10776
                     /locus_tag="VFH83_10185"
     CDS             9625..10776
                     /locus_tag="VFH83_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET6486778.1"
                     /translation="MSDAAKLLQELVRIGSINPMGRDLAGPTIGEARLTTFLETFLRS
                     LGLRPVRQKVHEGRENLYAVYQPSGARGLVLLEAHQDTVPVDNMTIDPFGGDSRDGKV
                     WGRGACDVKGGMAAILTAVRRLVAERSLRTGVMVAFTVDEEQTFAGVKKLLEAPLRPD
                     LAVAAEPTSLEIVIAHKGVARWKVRTTGQSCHSSYPERGVNAIYRMAPVIAAHERYAA
                     ILAAGRHHPLTGTATLSVGVIRGGTSVNTVPSWCEMEVDRRLLPGEDGEEAFRDAERY
                     LRETLPPQITVDLVEPWLLDPPLDTPQDAEVVSLARRAVAAVKGDPRVCGVPYGTDAS
                     KLALGGIPSIVLGPGDIAQAHTSDEWISIEQLDQAVEIYYRIVMEAGKG"
     gene            complement(10798..11838)
                     /locus_tag="VFH83_10190"
     CDS             complement(10798..11838)
                     /locus_tag="VFH83_10190"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HET6486779.1"
                     /translation="MRIEAVGVRIPTKKLTNDDILLELENQSNGINRTVLKTYQRMVK
                     GMFKMAGSEVRHIRDTEKDERASTMIKGAMQDALDKAGLTKDDIDLLIYCGVGKGFLE
                     PANAYFYAGTMGMRCSCFDISDACMSWIRALEISYQFLASGRYRRIMIANGEFNSDHG
                     FPDNFKVKSLRQVEYTFPTYTIGEAASATIVVASDHEWKFSFDSHPELCDLCTIPLRD
                     FDNFVEPTHRLAKNGLYNFVSYGREMFDDAKENLAALLKNVIKDVNEPDIYFPHAASD
                     AVYLAAGKKIGIDPDKLFSKVFPTYGNLVSASIPVAIDMALAEGRLKRGHKVVLCPAS
                     AGMVYGVVQFVY"
BASE COUNT         2049 a         3946 c         3936 g         2032 t
ORIGIN      
        1 ggacctgtac ttccgactgg agacgcatca cgtccatatc ccgccgctgc gggaccggct
       61 caaggacctg ccgtccctga tcgaccattt cgtgagccag gcctcggcgg agctgaaccg
      121 accggccccc gccgtccctg cggacgtcta cctgctgctc aagagccatc cgtttccggg
      181 aaacgtccgg gagctacggt ccttgatttt cgatgcggtg agccgacagg gtcccggatc
      241 cggcgaggcg tttcccgtcc ggttcctgaa gaccgccctg gggcaccgct ccacgcggat
      301 gcccgtctcg acgggagacg actacttcct cggtgctcta cagctcccca cgatcaagga
      361 agcgacggag aagctggtgg cggaggcgct gaaacgcgct gacgggaacc agtccgttgc
      421 ggctcggatc ctggggatca cgcctcaggc gctcagccag aggctccgtc gcgcgggaga
      481 ccgataggcc cggctgacca acgccatcaa gacgagcgtc gtgagccgac ggcccgccgc
      541 gcgtccaacg gggctacgat gcgtccgggt tcactgtcca cgcctgctcg gggatccgca
      601 ggaacgcctc tacgacgcgc ggatcgaagt gccttcccgc ctggctccgc agctcctctt
      661 ccaggtaccg gcgcgtcaga gccttccggt acgggcggtc gtagagcatg gcgtcctgag
      721 cgtcgatcag ggcgaagatg cgggcatgca gggggatggc ctccccgcgg agcccctggg
      781 ggtagccggt cccgtcgtag cgctcgtggt gacagaggac cacttccgcg gcatcgcgca
      841 ggaagtcgat gcggaggatc aaccggtgac ccaggaccgg atgcacccga acgaggtcca
      901 tctcctgcgg cgtaagcggt ccgggcttcg acagcacctg ctcgggtatc ccgatcttcc
      961 cgatatcgtg gagatacgca ccccacttga ggttgcgcag gtccgagggg agcaccccca
     1021 tgacctttcc cagcgtcacg gcgtttgccg cgaccctctg gcagtgctcc tccgtctcgg
     1081 ggtcccgcag atccagcgcc gctcccaggg cgttcacggt catgtcgtag atctcgtcga
     1141 tccgactctc ggcgcgcttc agttcgtccg tcatggagga gatcaagcca tcgatgtcgt
     1201 cctgatagct cctggcccgg tggcgatagc gccactcctg gacgctcttc tccaggcgct
     1261 cctcgaacaa ctccaggccg aagggtttgc agatgaaatc gtacgcgccc ttgcgcacca
     1321 cccggaccac ggtctccatt ccctgctcgg ccgtgatgat caccacgacg ctgctctccg
     1381 acacccgtcc cggctcgtcc aggagactgg tgcccgtgcc gtcgggaagc gccagatcca
     1441 ggaagaggat gccgtagcgc tcccgggtca gcgcctggcg cgcggccgcc agcgtctcgg
     1501 cggtgtcgca gaccaggccg cggctgcgga tggcgagccg caggctccgc agcaggatcg
     1561 tatcgtcgtc caccaccagc acgcgcggct ccaggggcgg catcgtcagg ccaaggcccg
     1621 acacgatgtc ggtctgggtg cttttcatga ttctcgccct tccttcgatg gctccggtgt
     1681 aggcaccttg cgcagcgtct tcacttccat ccgtcctgtc tcgttgtcga ataccaccgt
     1741 ccgtccatgt tcccctccca catcctcgaa cacaaccgga atcttgagct ccgccagcag
     1801 tcccttgacc gccgcgatgt tctgctcgcc gatcgtggcg atcccgcggt ccgtggcggc
     1861 gcggaacatg tttgccccgc ccgcgatccg cgccgtgatc cgctcccggc tccctcctcg
     1921 ctgaaggatc cgtcggagga gcgccggcag cgcggagtcg gcgtactttc cgacggaggt
     1981 gtcccgttcg agcgtcctcg gcagcatgat gtgcgcgagg ccggtgaccc ggcgcatctc
     2041 gtcgtacaac acgatcccga cgcaggaacc gagggtcgtc ttcaggaggt gggagttctc
     2101 caccaccgcc atttcagcca tctggaccag tcggaccgtt cgttccatgt ccttccccgc
     2161 aaccggcggc cgtgggaccg atggtggggg aggggctcag cgccgccacc cccgccgatc
     2221 ccgctgtcct gtaagaggaa tcggcggaac cagatctgtt gcgccaggat ataaacaaga
     2281 gtcgtgccat tcggggtgcc ttcctctaat gtattatgga ggaaagagat agagattgga
     2341 gtttcgaggt gcagaacgag gacttgctca acctatcttg agaggtttcc cagccgagga
     2401 gggctaactt cttgtcgagg aataaattag agctgctcta aagcttcctt gaagcggtgc
     2461 ggagcgtctc ggacttcgca agacccggag gaccgcgccg cagggactcc tttgacgtgc
     2521 cgctaccggg gtcgctttgc ccggttctgt tggactcgga tcctcacgag gtcttcgatg
     2581 agccggaccg gcagaggttc atccatgggg aactggaggg ttcccttgcc cttctggtag
     2641 ggggccaact gcagcttgaa cgaagccatg gcgccggtgc ccgggtagaa cccgatgtgc
     2701 ttcgcgtgcg ccgcgaagta gacgaggttc ccgttgaggt agaaggtcgg catccgatag
     2761 gaaatcttct ccgtggcatc cggcgccagg cggcggacca gacggcggat cgtctggagc
     2821 ctcttctgga ccggggcggg gcaggccagg atgtactcgt cgatggaacc gggtcctgag
     2881 cccttcttca cgaaagctcc tccttccgcc cgtggtcttt cgagctacgg gccggggggc
     2941 cttttggctt tggcctcggc aagccagtcc ttgacgaact gccccatgta gttctccgca
     3001 gtgtcttccg cgccgccgcc gaaccggctt gtgtgggtgc tcttgccgtt tcccaggagg
     3061 acccacgcgg tgtcgaacat cacgggacgg tacacggaat cgaagcacca ggccgtccag
     3121 cccacattgg ggtgagcatc gatatacgtg cgaaagatgc ggccgtagga agaggccgtt
     3181 ccgttcacgg gcatcgggcc gtccttctcg aagccccatt ccgtcatcat caccggcgcc
     3241 acgctgctta cgaagtcgat ccacggcggc atggccccgt gcacgtgagc gggatagaca
     3301 tggctggcat agacgatatt cgggccgctc agcaggtccg aggggcgctg gggcatgacc
     3361 tgggaccaga ccggcccgcc cacgatcacg aggttcccgg cgcccctggc ccggatgccg
     3421 gcgatccatc gctgcgccgt gaccttccag tccgaccagg atccgccgcc cggctcgttg
     3481 aagagctcga agaggacact gggctggttg gcgtaacgca cggccacccc aggccacgcg
     3541 cccttgccga gccagaatgc ctcggtgctc gattccacgg ccgcgtcccg ccagtcggcg
     3601 ccgatgtagt gccagtccac gatgacgtac ttcccacgtc ggatggcgtg ctggacggcc
     3661 gggtccagga tccgctcgaa gtaatcctgg ggcgcgtagt gcagccagcc gtgggagccc
     3721 gtctcgtccg tgacttccgg gtgaacgggc aggcggaaca cgtcgatgtc ccatccggag
     3781 gagcacccgc ggtcgatgat gtcgaacacg gtggtggggc cggattggtc gcggtccccg
     3841 ttgtggatcg cgtcgaggtc cgccaccgaa acgccgcgga gggtgacggc gtggcccgaa
     3901 gcgtcccgga tccacttgcc ctcgacgtgc agcgcgggaa gcggcgccgc ggcggaagag
     3961 gctgaggacc cagcggctcc ggcactctcg gctccggcgc cgatggccga cagtgccagc
     4021 aagggtgcca tgccgaggtg tcgaaggcga tgctgcgtca acacgccatt actgtagccg
     4081 ggatccggca gctgtcaagg aacgagcgct atcgcagcgg tcgcaatccg gctaaactcc
     4141 gcgccgtggc ggcttgcggc gcacgcccgt ggagcgcaaa ggagatcgga tgaacggtat
     4201 atctcgaagg tctatgagcc tcgcgatcgt tcttgccgcc gcggtctggg tctgcgcgcc
     4261 gggatgggcg ctggttacgg ccgatcccaa cgatgccgtc taccgggacc tcgaccgctg
     4321 gcagcagcaa ggatacctgg agcctctgcc gtcccttcgg ccctacccga cgcagctcat
     4381 cgtatcggca ctcaacgagg tggccgcgaa agcggggccg gtggacgcgg agctggcgcg
     4441 ctcgtatctc gacgctctgc gcggaggtct cctggggata ccggtgcacg cctcggcgac
     4501 ttacctccga gagcagaagc aggcagacgg cctgaaccgg ctgggtcttc aggcagacgg
     4561 cagcgggacc atcggtgacc tggtgggatt ctcggtccga ggcaggctgg tggccgacga
     4621 ttccaccgcg gaggtattcc cgcggtgggc ggatgactac gaggctaccc tggccaccca
     4681 gaccccgaat accgggacgc gctcctctct accgatctcg ttcggtgggc agcccttcgc
     4741 cggctacggt gtcctcaacg ggctggtgtc cttcggcacg gaccgcctgt ggatccaggg
     4801 cggcctggtc cagtcctcct ttggcccggt cctggagagc cccatcctgg gtccccaggc
     4861 gcctcaggca ggacatttca gcgccaccta tcgcggctcc tggtttacct acaccaacct
     4921 ggtggaagag ctcgtggcat actacgcggt ggacgcgagc gggaacacca gtctgatcac
     4981 ctcctcgagc gggagtccgt atccgcagaa gtaccttttc ctgcagacgt tcaacctctc
     5041 cccgtggccg tgggtccagg cggatatcgt ccaggcggtg gtttcgggcg gccggttctc
     5101 tcccacctat cttctcccgc ttctacccct gtacacgcag gcttacctcg gagactggga
     5161 caactcgttc gccggcggct acttccagtt caaccttccg ttcggaatcc ggccttccct
     5221 cctgctctat gtcgatgacc tggatctcaa gaagcttctg cagctcaact tcaacacgga
     5281 cggaaacaag gtggccatcc aggcaggcct ttcctgggca ccgccgcttt ccgtacccgt
     5341 tcaagcgagc cttacctaca ccgccatcac accgtacacc tacacccact tctcgtacga
     5401 cacgctgaac tatctccagt acacgaacct gggacagcag ctaggcagca tcctgccgcc
     5461 caactcggac cagtggacgc tgcgcgtcga ggtcatcccg ctctcctggc tgcggctgga
     5521 tctctgggga aagctgatcc ggcacgggaa cggttcggac tacggctcgg gtgtcgtcgt
     5581 gggaaccggg accgtctacg atgacggcgc cctgaccggc ccgatcacct tcctgaatca
     5641 gaacagcttc ctgacccagg cggtgctgga aaaggacgag cagatcggga tcgaaggcac
     5701 cgcggacctc gcgtacatcg ggcttcgggg agagctgtcc ttgagctata cgctggaagg
     5761 cgtgcagaac cgcgatctca atcccgcggc gccgaacgag ctgaacaaca tcctggggat
     5821 ccgcctctcc ttcggattct gagagatcag gtggcgggcc gccagtagcg ctcgataagg
     5881 cgcccggggc cggcgcccag gagtgaggag taggtgaggg cggcgcggcg ccacgcggcc
     5941 gcgtctccga agagcgcggg gagctgcgac ccgtagcagg cgatggcctg cgccttgacg
     6001 gaggcgcagt cggtcacatc gacggggtcg ggccgaagct ccaggactcc gccgcggctc
     6061 gaccggccgc tccccccgcg cgccctctgt ccggcaatcc gagccatgag cagcaccctc
     6121 aggatggcgt cctgcggcgg ccgcaccgaa tctcggatca cggggacggt caggattcct
     6181 tccagggcgc ggcgggagac gcgcaggatc gactcccttg cggccagatc cgccggcagg
     6241 ttgggcgagg ccacgcgcgc tttcacctgg gccagccgat gctcgaccat ggacggtacg
     6301 aacgagtacg ggatctcctc gaagtaggaa acgcgccccg cgaccgctgg agcgcgcatc
     6361 atgcgtcggg agacctcaaa gaggatccga tggtccacgt ggcccccgac gccgagtgga
     6421 aacacgagct cggcaccggg actggcccgg cagagccgct ccaggtcggc ctggagctcc
     6481 acgagcaagg ccgtgtcgct ccgccggatc cgcccgaaca gcgccaggaa cgagcggtag
     6541 cgtgggcttc ggccgatggc ctcttcgatg tccatccaca gggggacgac gcccaggcgg
     6601 tccatcgctc gacggtcttc ctcccgacgg gagctcatcg acgcgaatgg ggaaagggtt
     6661 ccgccggccc gtcggccctc cgcggccggg cttccggcga acggcgtcac gacgaccact
     6721 cgagcgccgg cccgcaccgc ggctgcgatg cggcccccac aggagcaggc ggcatcatcc
     6781 aggtgagggg aaaacaccag gaggtcgacg ccccgcggca ccgacgggcc gaggctcatg
     6841 tccggccggc cgcggctctc gaggatgggt cccgcaggtc ccgcaccagg agccggaaca
     6901 tccacctctt gtccgagcag tgcgcccaga gcgtgcaggg acgcgactcc agaagggttc
     6961 ctgccatcac cgcggcaaag gcgttgtgtc ctgcctcctc gatcaccact ccatgcgccc
     7021 ccacggctcc gaggctcttg atccggtcca cggcggtatc gaccatctgc cgtcccagcc
     7081 gcagcccccg gtactccggc gcaagcgcga tgtggaggtg agccgggtag cggccgcgcg
     7141 gcggctcggg gatctcccgc cagcttcggg tcatgaacca caggatgacg cggaaggtgc
     7201 tggtgttgtg gtactggagc gtgaacaggc gccagaggac gcggaccagg agccgcggga
     7261 tgtagcgggt caataccacc caattgtagc gatcgttgtc caggcagccg gcaatgtacc
     7321 cgacgacctt cccctccgct tcggccacga agcagcagcc gggctcgtgg tcgaggtgat
     7381 agtccacgaa gagggcaagg ggtatgtcct cttcctcgat gtagttgctc acgggatcac
     7441 cgcgctggcc gcccgcgcgg aagatgtctc ggaccgcttc gcggtcctcg cgccggtagg
     7501 ggcgcacgat ggcggtgggt ctgcgccgcg gggcagttgg gtcaggcatg gtcggccaac
     7561 ctcaggagat tcttgagctt gaagtaggac tcgaagacat ccttcaggaa cggggcatgc
     7621 caggtggctt cctggctgtg aaccacgtag cgctccacct ccgtgctctc ggcatgctcg
     7681 ccgaggtcga aaccgaagtg cggctcgaag cacgcggctg gcacgacggc aacggacgga
     7741 tccgatgagg accaccgatc gagcgcctgg gacaggagcg cggggcccgt gctgtacgtg
     7801 atgttcagct cccgcaggaa ccccaggcgg cggaccgcgt gaggcaagcg ccggatcgtc
     7861 tccgccagag gcggggcctg gggtgccgcg gccatcaccg cgttggtcac gatccgccgc
     7921 gcccctacca ccagccggat gagcttcatc tcgatggggc caaagggttg ctccgacacc
     7981 aggagaccgg cgcgggggta ttgccggagg agacggtcca tggggcgcag acactcggtg
     8041 tcgaggtccg cgtacacgcc tccctgctcg tagagaatga agtagcggcc cgagtccacc
     8101 cgctgatgaa ggtatcggta cccgtcgtag acggacagga accagggata ccggccggct
     8161 accagggccc ggatggaagc gtcatcccag aaccggtgct cccagcctgg attcctttcc
     8221 ttccaggacc tccgcaggtc gtcgtagcgc tcggggactt cccgctcccc ctggagccag
     8281 atctggtgga gcacgcgggg gatggccact agccccgtcc ccgcggagcc acggcaaggg
     8341 ccgaaaggac cgccccccat accgtgtccc agccgtgagc ctcggcgtgt cggcgggcgg
     8401 cctctcccat ccggcttcga agcgcgcagt cggagatgag ccgctccagg ccggcctcga
     8461 aggcaggcag gtccggcgcc acgaagccgt tctcaccgtc ggtgacgtcg tcccgggcac
     8521 cgcccgtcct ggccactacc accggcaacc ccgaggccat ggcctccagg atcaccatgg
     8581 gtccggcctc cgccagggac gcgaaagcga atacgtcgct ggaagcgtag gccgtggaaa
     8641 gctcgatgcc cgtgagccga ccggtgaaga gcacgtccgg caatcccatg gcctccaccg
     8701 cctcccgctc cgggccctcc cccacgatca ggagcgcggc ggaagggcga cgctgcttga
     8761 tcctcccgta catccgggcc aactcgggga ggttcttctc tcgggcgagc cttcccacga
     8821 aaaggatgac ctctttgccg gagagcccat ggcgggaacg gaactcctcc gatcgaaacc
     8881 gggggtgaaa gacctccgcg tccaccgcgc ggcggaagag caccgtgggg atggcgggcc
     8941 agctctgttg gacgaaccgt tctacggatt gggaaggcac caggatccgc gtgcactgac
     9001 gaaacaggcc cacgcggcgg tcgatcaacc attggaagaa ccggatcacg agcgcgaacc
     9061 ggaagatgtt cgccaggtag tactccgcca gcttggcgag ctggacatcg aaggatccga
     9121 ccaccggcac tcgcagcctt cgagacagga agaacgccgt gctgccgatt cctcgcagga
     9181 tgtacggggt cactgtaagg agcacctggg gctgaaacga ccggacctcg cgcagaaggg
     9241 cgcgcttggg actgcgcagg tagagccacg ggtacacgtt gtagccggga atggtcagcg
     9301 ccgcgcggta caccccgacg gtctccaccg atgagccgtt gcgctggatc ccctcccgcg
     9361 agcgatggca gatgagaagc gcgtgcccgg ggtttcgctc gacgtgccga gccagctcct
     9421 gcacgatgcg ggcaactcca tccgagtagt ggatgttcga gtccgtgaac agcgcgatcc
     9481 gcatccctgg ctccgattgt gtcgtgagca cttatagaca ccgcggtagg cagtgtcaag
     9541 gcgcgcgcgc tcgcaagacc ccggcgatgc accggccggt gtcccgttgc cgcggattgc
     9601 ggtatcctat cgacggagat ccgcatgagt gacgccgcaa agcttctgca agagctggtg
     9661 aggatcggga gcatcaaccc aatgggccgg gaccttgccg gtcctaccat cggggaggcg
     9721 cggctcacga ccttcctgga gacattcctg cgctccctgg ggctgcgacc tgtgcggcaa
     9781 aaggttcacg agggacggga gaacctctac gccgtttacc agccgtccgg cgcccgtggg
     9841 ctggtgctcc tggaggccca ccaggacacg gtgcccgtgg acaacatgac catcgatccg
     9901 ttcggcggcg actcccggga cgggaaggtg tggggccgcg gggcctgcga cgtgaagggc
     9961 gggatggcag ccatcctgac ggctgttcga cggctggttg ccgagcgatc gctcagaacc
    10021 ggcgtgatgg tcgccttcac cgtggatgag gagcagacgt ttgccggggt gaaaaagctt
    10081 ctggaggcgc cccttcgccc ggacctggcg gtggccgcgg agcccacgtc cctggagatc
    10141 gtcatcgccc acaagggcgt cgcgcgctgg aaggtccgca ctacggggca gagctgccac
    10201 agctcgtacc cggaacgcgg ggtgaacgcg atctaccgga tggcccccgt gatcgcggca
    10261 cacgagcgct acgcggcaat cctggcggcg ggacggcacc atccgcttac cggcaccgcc
    10321 acgctcagtg tcggggtgat ccgcggcggc accagcgtga acaccgtccc ttcgtggtgc
    10381 gagatggaag tggatcggag gctcctgccc ggtgaagacg gtgaggaggc cttccgagac
    10441 gccgagcgct acctgcggga gaccttgccg ccccaaatca ccgtggatct ggtggagccc
    10501 tggctgctgg atccgccgct ggacacgccg caggacgccg aggtggtgtc gctggcgcgc
    10561 cgggccgtag ccgcggtcaa aggcgatccc cgcgtctgcg gggttcccta tgggaccgat
    10621 gcctccaagc tcgcgctggg gggcattccc tcgatcgtcc tgggtccagg ggacatcgcc
    10681 caggctcata cctcggatga atggatctcg atcgagcagc tcgaccaggc ggtggaaatc
    10741 tactatcgga tcgtcatgga agcagggaag ggctgagacc cgcgaggcgg cggagcctca
    10801 gtagacgaac tggaccaccc cgtacaccat cccggcgctg gccgggcaca ggacgacctt
    10861 gtgtccccgc ttgagtctgc cctcggctag cgccatgtcg atggctaccg ggatgcttgc
    10921 ggacaccagg ttgccgtagg tcgggaacac cttgctgaag agcttgtccg gatcgatccc
    10981 gatcttcttc cccgcggcca ggtagacagc gtccgaagcc gcgtgcggga agtagatgtc
    11041 cggctcgttc acgtccttga tgacgttctt gaggagcgct gccaggttct ccttggcgtc
    11101 atcgaacatc tcccggccgt aggagacgaa gttgtacagg ccgttcttgg ccaggcgatg
    11161 ggtcggctcg acgaagttgt cgaagtcccg caaggggatg gtgcacaggt cacacagttc
    11221 ggggtgggaa tcaaaggaga acttccactc gtggtccgaa gcgaccacga tggtggcgga
    11281 ggcggcctcg ccgatggtgt aggtggggaa cgtgtactcc acctgtcgga ggcttttcac
    11341 cttgaagttg tcggggaacc catgatcgct gttgaactcc ccgttggcga tcatgatacg
    11401 gcgataccgg ccgctggcca ggaactggta ggagatctcc agggcccgaa tccagctcat
    11461 gcaggcgtcc gagatgtcga agcacgagca gcgcatgccc atggtgcccg cgtagaagta
    11521 cgcgttggcc ggctccagga atccctttcc cacgccgcaa tagatgagga ggtcgatgtc
    11581 gtccttggtg agccccgcct tgtccagcgc gtcctgcatg gcgcccttga tcatggtgga
    11641 ggcccgctcg tccttctccg tatcccggat gtgccggacc tcggaacccg ccatcttgaa
    11701 catccccttg accatcctct ggtaggtctt gaggacggtg cggttgatgc cgttactctg
    11761 gttctcgagc tcgagcagaa tatcgtcgtt cgtcagcttt ttagtgggaa tcctaacacc
    11821 aacggcttcg attctcatgg gttccctaca ctatactatc tttgtcggat caaacacaac
    11881 ccgcgcggcg gctggctccg gttggtgtca acgcaccgcg gctggcaccc gccggggtcg
    11941 cgccgctaca gggccccccg ggg
//
LOCUS       DASRZS010000416         4690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_615724, whole genome shotgun sequence.
ACCESSION   DASRZS010000416 DASRZS010000000
VERSION     DASRZS010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615724"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..947)
                     /gene="phnC"
                     /locus_tag="VFH83_10195"
     CDS             complement(183..947)
                     /gene="phnC"
                     /locus_tag="VFH83_10195"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006211085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HET6486780.1"
                     /translation="MGPILTIRNLTKRYGEVTALNGVSLSVHHGEFVSVIGRSGAGKS
                     TLLRCINRMVDASSGEILLNDVNVCTLTRAQLRKLRSRVGMIFQHYNLVQRLTVLENV
                     LHGRLGYKRTLPGMVGHYTHAEKLLAVRTIAMLGLVDQMYKRCDELSGGQKQRVGIAR
                     ALVQEPHLILCDEPIASLDPSSAKVIMDHLRMVCDDLGITILVNLHQIDVALKYSDRI
                     LGVKEGRVVYDGSAQNLARDTIYEIFGSEAGELIND"
     gene            complement(978..2018)
                     /locus_tag="VFH83_10200"
     CDS             complement(978..2018)
                     /locus_tag="VFH83_10200"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HET6486781.1"
                     /translation="MKAPARRVWVVAVLALFAAAAAFASGSKEALTFVWIPNDSVPEA
                     KTFRTAIDKVISDAIGRPVVDKLTTDYNIAIEAMATDNAALAYFGGLQYVQTHQKNAK
                     VIPLVTNSGDSGTMSDAQYFSRISVKTGDAPQYMTSGSYSIENIQGKRFAFVSNSSTS
                     GFLFPSSGIVGYFSKKPAWSALKPNDLIEGGQGKFFSQVVFGGSHQGSAAALLTDKVD
                     AAAFDDTDLSAYVDLVSGTESTPGAVYKVKDGAAQPFDTLVGKSFTVLWSIPVLNGPI
                     CANQNLLTADELKKIVAALTADSTSMNPSIFFPPDYKGPNKADFTQTGKVKFLAVDDS
                     TYQPIRDKLQGQ"
     gene            complement(2101..3267)
                     /gene="phnW"
                     /locus_tag="VFH83_10205"
     CDS             complement(2101..3267)
                     /gene="phnW"
                     /locus_tag="VFH83_10205"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HET6486782.1"
                     /translation="MSASDIPANPYVLLTPGPLTTTRTVKEVMLRDWCTWDNDYNEIV
                     QEIRRHLVRIAVDRLHGAYTSVLMQGSGTFCVEATLGTAVPEDGKVLILANGAYGERM
                     AQIAARLRIPHAVLRTEETIQPDPLQVDHALAADPSITHVALVHCETTTGILNPLEGI
                     ASIVREHGRIFIVDAMSSFGGIPMDIGELGVDFLISSSNKCIQGVPGFGFVIARRASL
                     EACEGRARSLALDLYDQWRTMEKERGKWRYTSPTHVVRAFAQALTELDEEGGVRARYA
                     RYRANHQELLRGMGRLGFPCLVPEELQSPIITSFIEPPADSGYSFSRFYAELKSRGFV
                     IYPGKVSRAATFRIGTIGDVHPETIRRLVQAIEECLFWESPRNSPEAQSMYTES"
     gene            complement(3254..4093)
                     /gene="phnX"
                     /locus_tag="VFH83_10210"
     CDS             complement(3254..4093)
                     /gene="phnX"
                     /locus_tag="VFH83_10210"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016827 - hydrolase activity, acting on
                     acid carbon-phosphorus bonds [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0019635 - 2-aminoethylphosphonate catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HET6486783.1"
                     /translation="MSSKGSIAAVVFDWAGTIVDFGCFAPTVSIVETFADRGIQVTLA
                     EARGPMGLEKKEHLRRLLDQEGIRRQWRATTGSDPTPSDAQALYEELEPRLFQAVKRY
                     SELVPGARELTAELHASGIGIGSSTGYLRPLMDVVCQEAAAQGLLMDEVICPSDVPAG
                     RPYPWMCFLNAMRLNACPMSRVVKIGDTPADMQEGVNAGAWVIGVTLSGNEVGLSASE
                     AAEMDPADREALLRAAETRLLAAGAHYVTESVATCREVLTRIDERIARGEQPSAERVH
                     ERV"
     gene            4253..>4690
                     /locus_tag="VFH83_10215"
     CDS             4253..>4690
                     /locus_tag="VFH83_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6486784.1"
                     /translation="MSLSQRWLQEAQAVAPGRVVTDPALMEPYGHDESPVGTWQRLPE
                     AVVKPADEAEVAAILRLCGQHGVPLTVRGAGTGLAAGCVPAPGGIVLSMELLNRVIEA
                     DPSNRTVTVQAGVTMKRLYEEVDRMSLYFPPHPGDEGAFVGGAA"
BASE COUNT          798 a         1493 c         1571 g          828 t
ORIGIN      
        1 ggacggcgag cagcaccagc gtgaatcgat tgcgccgccg acggaacatc tcgtcgggag
       61 acgggcacga ggagaggtcc atagacgggc ttctccgacg gacgcgcctc ggcccgcccg
      121 accggtccga caggtcggga ggttccccag ggacgacttc caccagttct tccatggcgc
      181 cctcagtcgt tgatgagctc gccggcctcg gacccgaaga tctcgtagat cgtgtctcgg
      241 gcgaggttct gcgcgcttcc gtcgtagacg acccgtccct ccttgacgcc caggatgcgg
      301 tccgagtact tcaaggcgac atcgatctgg tgaaggttca ccaggatcgt gatccccagg
      361 tcgtcgcaga ccatgcgcag gtgatccatg atgactttgg cggagctggg atccagggag
      421 gcgatgggct cgtcacagag gatcaggtgg ggctcctgga ccagcgcacg ggctatgccc
      481 acgcgctgct tctggccgcc ggagagctcg tcacaccgct tgtacatctg gtccacgaga
      541 ccgagcatgg cgatggtccg cacggccagc agcttctccg catgggtgta gtggcccacc
      601 atgccgggca gggtacgctt gtagcccaac cggccgtgaa gcacgttctc cagcacggtg
      661 agcctctgca ccaggttgta gtgctggaag atcatgccga cccgggagcg gagcttccga
      721 agctgagccc gggtcagcgt gcagacgttg acgtcgttca ggaggatctc cccgctcgat
      781 gcatcgacca tgcggttgat gcatcgaagc agggtggact tgccggcgcc ggaccggccg
      841 atgacggaga caaactcacc gtggtgaacc gagaggctca ccccgttcaa ggcggtgacc
      901 tctccgtagc gtttcgtcag gtttcggatc gttagaatgg gtcccacgtc ccgcctccgg
      961 cgccgttcgt ccgctgtcta ctgaccctga agcttgtctc ggatgggctg gtaggtgctg
     1021 tcgtctacgg ccaggaactt caccttgccc gtctgggtga agtccgcctt gttcgggccc
     1081 ttgtagtccg gcgggaagaa gatggacgga ttcatggacg tggagtccgc ggtaagggcg
     1141 gcaacgatct tcttgagctc gtccgccgtc aacagattct ggttggcgca gatgggaccg
     1201 ttcagaaccg ggatggacca gagaaccgtg aagctcttgc ccaccagcgt gtcgaacggc
     1261 tgggcggctc catccttgac cttgtaaacc gctccgggcg tgctctcggt accggagaca
     1321 agatccacgt aggcggatag atcggtgtca tcgaacgccg cggcatcgac tttgtccgtg
     1381 aggagcgcgg ccgcggatcc ctggtgagaa ccgccaaaga ccacctggct gaagaacttg
     1441 ccctggcctc cctcgatcag gtcattcggc ttgagggcgg accaggccgg cttcttggag
     1501 aaatagccga cgatcccgga ggacgggaac aggaacccgg aggtcgagct gttggagacg
     1561 aacgcgaagc gcttgccctg gatgttttcg atggagtagg agcccgaggt catatactgc
     1621 ggggcgtccc cggtcttcac ggagatcctg ctgaagtact gggcgtcgct catggttccg
     1681 gaatcaccgg agttcgtcac cagcgggatc accttggcgt tcttctggtg ggtctgcacg
     1741 tactggagac cgccgaagta ggccagggcg gcgttgtcgg ttgccatggc ctcgatggcg
     1801 atgttgtagt ccgtggtcag tttgtccacc acggggcggc cgatcgcgtc ggagatgacc
     1861 ttgtcgatcg cggttcggaa tgtcttggcc tccggaacgg aatcgttggg gatccagacg
     1921 aaggtgaggg cctctttcga gccggacgca aaggccgctg ccgccgcgaa gagcgccagg
     1981 accgcgacca cccagacacg tcgagcaggt gctttcatcg atacctccct tgggaatcgg
     2041 atgggccgcc gaagccgaca agcggccccg gttcggtgat cgcaatcttg ctccgtctcg
     2101 ttaactctcc gtgtacatcg attgagcttc cggtgaattc cgcgggcttt cccagaacag
     2161 gcactcctcg atggcctgca cgagccggcg gatggtttca ggatggacgt ccccgatcgt
     2221 tccgatgcgg aacgtggccg cccgcgagac cttgccaggg tagatgacga acccacgcga
     2281 cttgagctcc gcgtagaacc gggagaacga ataccccgag tccgcgggcg gctcgatgaa
     2341 cgaggtgatg atcggggact gcagctcctc cgggaccagg cacgggaatc cgagccggcc
     2401 catgccgcgg agcagctcct ggtggtttgc ccggtagcgg gcgtatcggg ccctgacccc
     2461 gccttcttca tccagctccg tgagggcctg ggcaaacgcc cggaccacgt gcgtgggaga
     2521 cgtgtatctc cacttgcccc gctccttctc catggtccgc cactggtcgt agaggtccag
     2581 ggccagggaa cgggcccggc cctcacacgc ctccagggag gcacgcctgg caatcacgaa
     2641 gccgaagccg gggacgccct ggatgcactt gttcgagctg ctgatgagga agtcgacacc
     2701 gagctccccg atatccatcg ggatgccgcc gaagctgctc atggcatcca caatgaagat
     2761 gcgtccgtgc tcccgcacga tggaggcgat cccctccagg ggattcagga ttcccgtggt
     2821 cgtctcgcag tggacgaggg caacgtgcgt gatcgatgga tccgccgcca gggcatgatc
     2881 cacctggagg gggtcgggct ggatggtctc ctccgtgcgc agaacggcat gagggatgcg
     2941 cagccgggcc gcgatctggg ccatccgctc cccgtaggcg ccgttggcca ggatcagcac
     3001 cttgccgtcc tcgggcacgg cggttcccag cgtggcctcc acgcagaagg tgccggagcc
     3061 ctgcatcagg acgctggtgt aggcgccgtg aagccggtcc accgcgatcc gcaccaggtg
     3121 ccgtcggatc tcctggacga tttcgttata gtcgttgtcc cacgtgcacc agtcccggag
     3181 catgacctcc ttgaccgtac gggtcgtagt cagcgggccc ggcgtgagaa gcacgtaggg
     3241 gtttgccggg atgtcagacg cgctcatgga cccgctccgc gctgggctgc tcgccgcgcg
     3301 cgatccgctc gtcgatcctg gtcagcacct ctcggcaggt cgccacgctt tccgtcacgt
     3361 agtgagcacc cgccgcaagg agccgcgttt ccgccgcccg caggagcgcc tcccgatccg
     3421 cgggatccat ctctgccgcc tccgacgccg agagccccac ctcgttgccg gagagcgtga
     3481 cgccgatcac ccaggcaccg gcattcacgc cctcctgcat gtcggctggg gtatcaccga
     3541 tcttcacgac gcggctcatc ggacaggcgt tcagccgcat ggcgttgagg aagcacatcc
     3601 acgggtaagg ccgcccggcg gggacgtcgg acggacagat gacctcgtcc atcaggaggc
     3661 cctgggccgc cgcctcctgg cacacgacgt ccatgagggg tcgcaagtag ccggtgctgg
     3721 aaccgatgcc gatgccggag gcgtgcagct ccgccgtcaa ctcccgagcg ccaggaacca
     3781 gctcgctgta gcgtttcacg gcctggaaga gcctcggctc caactcctcg taaagcgcct
     3841 gcgcatccga cggcgtggga tcggagccgg tcgtcgcccg ccactgacgg cggatcccct
     3901 cctggtccag gagccggcgg aggtgctctt tcttctccag gcccatgggg ccgcgcgcct
     3961 cggccagggt gacctggatg ccgcggtcgg cgaaggtctc tactatagaa acggttggcg
     4021 cgaagcagcc gaaatccacg atggtcccgg cccaatcgaa gaccacggcc gcgatggagc
     4081 ctttgctact cacgacggca agcatggacg agaagcgtta ggactctgtg aggatcggga
     4141 gaatttccgg tgaagtgcgc ggcggcggag aagcgcgggt tcccgtccgt tgaccacccg
     4201 gcgcgccgcc gctattgtgg agcgcactcg gggcaggatc ggaggatctc tcatgtcgtt
     4261 gagccagcga tggttgcagg aggcgcaggc agtcgcgccg ggtcgggtgg tgacggaccc
     4321 tgccctgatg gagccctatg ggcacgacga gtcccccgtg gggacctggc agaggctccc
     4381 ggaagccgtc gtgaagcccg cggacgaagc ggaagtggcc gccatcctcc gcctgtgcgg
     4441 ccagcacgga gtgccgctca ccgttcgggg cgcgggaacc gggcttgccg ccgggtgcgt
     4501 cccggcgccg ggcggaatcg tcctatccat ggagctcctg aaccgggtga tcgaggccga
     4561 tccctccaac cgtaccgtca cggtgcaggc cggtgtcacc atgaaacggc tctacgagga
     4621 agtggaccgc atgtctctct acttcccgcc ccatccgggg gatgagggcg cgttcgtggg
     4681 cggcgccgcg
//
LOCUS       DASRZS010000417        10314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_615870, whole genome shotgun sequence.
ACCESSION   DASRZS010000417 DASRZS010000000
VERSION     DASRZS010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10314
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615870"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /locus_tag="VFH83_10220"
     CDS             <1..1677
                     /locus_tag="VFH83_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HET6486785.1"
                     /translation="IDNSVDEFIMGEGKVIDIARKDGMVTVRDYGRGIPLGKVIDCVS
                     KVNTGGKFNDDVFQFSVGLNGVGTKAVNALSSEFEVVSYREGKFVQGLFRQGKLIGQK
                     EGKDAKTRRGTLIRFSPDPVIFPGFTWNDEFVLKRLRYYAFLNAGLTLVYNGEKVSSK
                     DGLKDLLVEEIGEENTLYDIAHYQGEHLEFAFTHTGTYGESYFSFVNGQFTVDGGTHQ
                     TAFRLGVLKAIDDYAGTTFSAEDVREGMIGAVAIKLKDPVFESQTKHKLGNNEIRGWV
                     ADEVKKETLVWLSRNEADAKRLVEKIRTNERIRKELATIKKQARELARKVELHIPKLT
                     DCKIHFDDENDERREESTVFLTEGDSAGGPMIQARDVYTQAIFSLRGKPLNVFGLKRD
                     TVYKNEELYNIMRALGIEDGLDGLRYNRVVIATDADVDGMHIRNLLLTFFLHYFEDLV
                     LKEHLYILETPLFRVRTRAETVYAYSEAERDTAMKRLTNSEVTRFKGLGEINPSEFKQ
                     FIGPEMRLTPVQVPSMGGVRRTLEFLMGKNTPERRKFIVNNLHVDVLEGA"
     gene            1721..3664
                     /locus_tag="VFH83_10225"
     CDS             1721..3664
                     /locus_tag="VFH83_10225"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF007209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET6486786.1"
                     /translation="MSPLSQLKPLMEGYFLDYASYVIMDRAIPELRDGCKPVQRRILH
                     TLYQMHDGKFHKVANVIGETMKLHPHGDASIGDALVVLANKEYFIEKQGNFGHLITGH
                     AAAAPRYIECRLTPLAMETLFNPALTQFVPSYDGRRQEPKELPAKLPVLLMLGAEGIA
                     VGMSTTILPHNFSELLTGIIALLKGEKVELYPDFLQGGSIDVSEYNDGSGKLRVRARI
                     DKVSGVQKLVIREIPYSTTTTALIASIDAAVQKGKVSVSKINDFTTDKVEIELSLSRG
                     ADAEKVIDQLYAHTDCEVSILSNMVIIRDNRPVEIATSDYLKEFSGILKRQIKAELEH
                     ELARLEDRRHWLTLEQIFIENRVYKRIEKAGTEEAVQREVRAGMKPFSKQFIRPMTDD
                     DVTRLLDLKIRRISAYDIKRNRGEINDIVAAIKQANAKLKNLTKTAITWVQGILEKYG
                     KGYPRRTEIETFHTVDKKAVAKANLRLSWDRDTGFFGSSVRANEFVMNVTAYDKILVV
                     TADGTYRIGPPPEKAFMPATVLHAEVFDPEKGAYLAMVWLDAARTPWGKRVKIERFIN
                     NKVYSLVKEGAAGIAYLSADRKAPGVVQIDFVPAKRAKLKSLTIDLSKVQESGLSARG
                     TRLATKAVENVILAPEKPKAGKK"
     gene            3809..4546
                     /locus_tag="VFH83_10230"
     CDS             3809..4546
                     /locus_tag="VFH83_10230"
                     /inference="COORDINATES: protein motif:HMM:NF004846.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6486787.1"
                     /translation="MPEANARFELLDLASLQSIAAFAARMRDQRPSIDLLINNAAVMA
                     PPHRQVTTDGFELQFGTNYLGHFALTARLLSLLRKGKNPRIVNVSALAARDGVIDFGD
                     LQAERSYRPWAVYCQSKLANLLFSFDLQRRSDAAGWGLTSIAAHPGISRTDLVSNGFG
                     KNSVASILMRLVGALFFQAASQGTLPTLFSATSPLAKPGAYHGPDGRNEMRGTPAASK
                     APPQAEDADVARRLWELSERLVRVTFE"
     gene            complement(4595..5446)
                     /locus_tag="VFH83_10235"
     CDS             complement(4595..5446)
                     /locus_tag="VFH83_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486788.1"
                     /translation="MQGYRFIFSDSDLLYLVKTLVPQTQDASRMIRVLRDDPEILEGM
                     LKDPKLAHRLLDDPQSILQVSPSLLFAVLLRRVRADLEKERFTLEHSGGRSLALFDTP
                     QIVALLDRGELLSYLTEMLVSFVRVSSFSTVVRVKHGVWRRVRFSDFDLDSLLSYASA
                     LEEARRFPVYKRAADVCLFTLGIMPPEEADPLRSASVRPAESHSAAGAVPWESLRVSR
                     RRTRQDYLTQGSSFYRLASRHPDAEVRQLSSVMGTLAEKFALAVKPLSTMSSKYLAPF
                     KEKVFLQ"
     gene            5570..6016
                     /locus_tag="VFH83_10240"
     CDS             5570..6016
                     /locus_tag="VFH83_10240"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486789.1"
                     /translation="MLEKDQLGGGKVRVTFLMPALEGVGTLSVCGEFNDWSPAAAPMT
                     RAADGTWSAALTLEAGRSYQFRYRDDRGGWHNDPAADTYVPNDFGSENSVVDLRDKPS
                     GAGAGTAPRKTAPSKVAPAKPAPSKAALQKPAPKKTSPKKPRGKGQ"
     gene            6049..7107
                     /locus_tag="VFH83_10245"
     CDS             6049..7107
                     /locus_tag="VFH83_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HET6486790.1"
                     /translation="MLFTTLALRGIPLRNRIVVSPMCQYSCTDGMATDWHLVHLGSRA
                     VGGAALVMTEATAVEARGRISAADLGIWDDTHVPPLERIAHFIHAQGALAGMQLAHAG
                     RKASVKAPWQGGAPVVVGDGGWEVVGPSAIPFDEASPVPHVLGVEEVRGVVREFARAA
                     SRALVAGFQALEIHAAHGYLIHEFLSPISNTRSDAYGGSFDNRIRLLREIVAEIRKRM
                     PESFPLLVRISATDWTTGGWDVEQSVELARRLKEEGVDVIDCSSGGNVKGVTTAIGPG
                     YQTPFAERVRREAQIKTAAVGLITSAQQADHVLRTGQADLVVMARQLLRDPYWPLRAA
                     AELRQEIAWPNQYLRAKA"
     gene            complement(7120..8349)
                     /locus_tag="VFH83_10250"
     CDS             complement(7120..8349)
                     /locus_tag="VFH83_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HET6486791.1"
                     /translation="MGYQLLNGIPVWGTHDEATLAQIRRCASDDRVAAAALMADGHKG
                     YSMPIGGVVAYRDAVSPSGVGYDISCGLKAVRTEVTASQVRPEIKRIMDAVAATVVFG
                     LGRSSGKSIDHEVFDDPGWREVRPLEPLRRLAREQLGTVGSGNHFVDLLEDEDGRLWV
                     AAHFGSRGFGYKTASGFLNLAAGRSFDARAPGEGVDQPATVLPLDGALGQDYWRAMTL
                     AGRYAYAGRDIVVDQVLSLLGAHPVDVVHNHHNFAWIEEHHGERLVVVRKGATPAWPG
                     QRGIVGGSMGDISVIVEGVAGPESEEALHSTVHGAGRLMSRTQAAGKRRWAKKGGRRV
                     QQVVRPGLVSRQMMMQWMEREGVELRGAGTDESPHVYRRLPEVLAAHQGTIRVLHTLR
                     PIGVAMAGEDVLDLYRD"
     gene            complement(8390..9604)
                     /locus_tag="VFH83_10255"
     CDS             complement(8390..9604)
                     /locus_tag="VFH83_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486792.1"
                     /translation="MTTRSIVESALEQGKGILRLAPAWVPRSFCVPGRRIKLHPDDYY
                     ALGGDRGGIDERWFASTTPADNGPKTSPNEGLSFIVFEDRGTTEKVLLRDAVAELKEK
                     IIGQRIWSAHQGWPMFSKFFDNKGPLPHHVHHRDEQAKLAGRTGKPEAYYFPVQLNNY
                     GADFPYTFFGLKPGTTKDQVRDCLRNFTKGDNKITNLSTAYRLEPGTGWDVAGGILHA
                     PGTLCTYEPQKASDVFSMFQSLTGDSIVPEELLWKDVPKDRVGDIEFLISLLDWEANV
                     DPTFTANHFMRPLPVRPVEQTRAEGYEESWITYRSSAFSAKELRVLPGKTVTIKDAAA
                     YGMILLQGHGTMGPWDIDSPAVIRFGQLTMDEFFVTEDAAKAGVKIANPSRTDPLVML
                     KHFGPGNPDLKL"
     gene            9902..>10314
                     /locus_tag="VFH83_10260"
     CDS             9902..>10314
                     /locus_tag="VFH83_10260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET6486793.1"
                     /translation="MNRATRTARLSILSNTLLIALKFTVGLVSGSVSVLSEATHSLMD
                     LVAAFMAFFSIRMAQRPADPQHPYGHEKIENVSGVLEALLILAAVCLIVYESVKKLIT
                     GEPAQRLELGLLVMVVSGVVNLFVSRRLYRVAAEEG"
BASE COUNT         1895 a         3352 c         3415 g         1652 t
ORIGIN      
        1 atcgacaaca gcgtggatga gttcatcatg ggggagggaa aggtcatcga catcgcccgc
       61 aaggacggga tggtgaccgt tcgggactac ggacggggaa tccccctggg caaggtgatc
      121 gactgcgtca gcaaggtgaa cacgggcggg aagttcaacg acgacgtatt ccagttctcc
      181 gtcggtctga acggcgtggg aaccaaggcc gtgaatgcac tctcctcgga gttcgaggtg
      241 gtgagctatc gggagggcaa gttcgtccag ggactgttcc gccagggcaa gctgatcggg
      301 cagaaagagg gcaaggacgc caagaccagg cgcggcacgc tcatccggtt cagtccggac
      361 ccggtcatct tccccgggtt cacctggaac gacgagttcg tgctcaagcg cctgcgctac
      421 tacgcctttc tcaacgcggg gctcaccctc gtctataatg gagagaaagt aagcagcaag
      481 gacggtctga aggacctcct ggtcgaggag atcggggaag agaacaccct ctacgacatc
      541 gctcattacc agggagagca cctggagttc gccttcaccc atacgggcac ctacggagag
      601 agctacttca gcttcgtgaa cggccagttc accgtggacg ggggaacgca ccagaccgcg
      661 tttcgcctgg gcgtgctcaa ggccatcgat gactacgcgg gcaccacctt ctccgcggag
      721 gacgtgcggg aagggatgat cggcgccgtg gccatcaagc tcaaggaccc cgtcttcgag
      781 agccagacca agcacaagct gggaaacaac gagatccgcg gatgggtggc ggatgaggtc
      841 aagaaagaga ccctggtgtg gctgagccgc aacgaggcgg acgccaagcg cctcgtggag
      901 aagatccgca ccaacgagcg catccgcaag gagctggcca ccatcaagaa gcaggcgcgg
      961 gagctggcgc gcaaggtgga gctccacatc cccaagctca ccgactgcaa gatccacttc
     1021 gatgacgaga acgacgagcg ccgggaggag tccaccgtct tcctgacgga gggggactcc
     1081 gccggcgggc ccatgatcca ggctcgggac gtgtataccc aggccatctt ctccctccgg
     1141 ggcaagccgc tcaacgtctt cgggttgaag cgggacaccg tctacaagaa cgaggagctg
     1201 tacaacatca tgcgcgccct gggcatcgag gatgggctgg acgggcttcg ctacaaccgg
     1261 gtggtcatcg ccacggatgc ggacgtggac ggcatgcaca tccgcaacct gctcctcacc
     1321 ttcttcctgc actacttcga ggacctggtg ctgaaggagc acctctatat cctggagacc
     1381 ccgctttttc gggtgcggac ccgcgcggag acggtctacg cctacagcga ggcggagcgg
     1441 gatacggcca tgaagcgcct caccaactcg gaggtcaccc ggttcaaggg gctgggagag
     1501 atcaacccat cggagttcaa gcagttcatc ggacccgaga tgcggctgac ccccgtccag
     1561 gtgccgtcca tgggcggcgt gcgtcggacg ctggagttcc tgatgggcaa gaacacgccg
     1621 gagcggcgca agttcatcgt caacaacctg cacgtagacg tgctggaggg agcatgaggg
     1681 ccgatcgccg ggacgcggac ggggagggga gggccacggc gtgagccccc tgagccagct
     1741 caagcccctc atggaagggt acttcctcga ctatgccagc tacgtgatca tggaccgcgc
     1801 catccccgag ctgagggacg ggtgcaagcc cgtgcagcgg cgcatcctgc acacgctcta
     1861 ccagatgcat gacggcaagt tccacaaggt ggccaacgtc atcggggaaa cgatgaagct
     1921 gcatccccat ggggacgcct ccatcgggga cgccctggtg gtcctggcca acaaggaata
     1981 cttcatcgaa aagcagggga acttcgggca tctcatcacg ggccacgcgg cggccgcgcc
     2041 ccgatacatc gaatgccggc tcactcccct ggccatggaa acgctcttca acccggccct
     2101 cacgcagttc gtgcccagct atgatggccg gcgccaagag cccaaggagc tgcccgccaa
     2161 gctgccggtc ctcctgatgc tcggggcgga ggggatcgcg gtgggaatgt ccaccaccat
     2221 cctgccccac aacttctcgg agcttctgac cgggatcatc gccctgctca agggagagaa
     2281 ggtggaactg tacccggact tcctccaggg gggatcgatc gacgtctcgg agtacaacga
     2341 cggttcggga aagcttcggg tgcgcgcccg gatcgacaag gtgagcgggg tccagaagct
     2401 cgtcatccgg gagatcccct actccaccac caccaccgcc ctgatcgctt ccattgacgc
     2461 ggccgtgcag aagggcaagg tcagcgtctc caagatcaac gacttcacca cggacaaggt
     2521 ggaaatcgag ctgtcgctct cccggggcgc ggacgcggag aaggtcatcg accagctcta
     2581 cgcgcacacg gactgcgagg tgtccatcct ttccaacatg gtgatcatcc gggacaaccg
     2641 gcccgtggag atcgccacct cggactacct caaggagttc tcggggatcc tcaagaggca
     2701 gatcaaggcg gagctggagc acgagctggc ccgtctggag gatcggcgtc actggctgac
     2761 ccttgagcag atcttcatcg agaaccgggt gtacaagcgc atcgagaagg cgggcacgga
     2821 agaggccgtc cagcgtgagg tccgcgccgg catgaagccg ttctccaagc agttcatccg
     2881 tcccatgacg gatgacgacg tgacgcgtct gctggacctc aagatccgcc ggatctccgc
     2941 gtatgacatc aaaaggaacc ggggagagat caacgacatc gtcgccgcca tcaagcaggc
     3001 caacgcgaag ctcaagaacc tgaccaagac cgccatcacg tgggtccagg ggatcctgga
     3061 aaagtacggc aaggggtacc cccggcgcac ggagatcgag acgttccata cggtggacaa
     3121 gaaggcggtg gccaaggcca acctgcgcct ctcctgggac cgggacactg ggtttttcgg
     3181 ctcctcggtc cgcgccaacg agttcgtgat gaacgtgacc gcctacgaca agatcctggt
     3241 ggtgacggcg gatgggacct atcgcatcgg gccgccgccg gagaaggcct tcatgcccgc
     3301 gacggtgctc cacgcggagg tcttcgaccc ggagaagggc gcctatcttg ccatggtctg
     3361 gctggacgcc gcgcgcacac cctggggcaa gcgcgtgaag atcgagcggt tcatcaacaa
     3421 caaggtctac tccctggtga aggagggagc ggcggggatc gcctatctct ccgcggaccg
     3481 aaaggccccg ggcgtggttc agatcgactt cgtgccggcc aaaagggcca agctcaagag
     3541 cttgaccatc gatctttcca aggtgcagga gagcgggctc agcgcccgcg gcacccgtct
     3601 ggccaccaag gcggtggaga acgtcatcct ggcgccggag aagccgaagg ccgggaagaa
     3661 gtagagctcg cacggagcag cccatccggg ctcgtggggt aatttcgggg gcagacgctt
     3721 gggagctcgc ttcgcggtct gatggggcgc cgtcgcgggt cgcaacgcgg cgaagggcgc
     3781 tgcggcggtt caggggatcc gcaagggcgt gccagaggcg aatgcccggt tcgagctgct
     3841 ggacctcgcc agtctgcaat cgatcgcggc tttcgcggcg cggatgcgcg accaaaggcc
     3901 gagcatcgat ctgctcataa acaacgcagc cgtcatggcg ccgccccacc gccaggtcac
     3961 caccgacggc ttcgaattgc agtttggcac caactacctg ggtcacttcg cgctgacggc
     4021 ccgacttctc agcctccttc gcaagggcaa gaaccctcgc atcgtcaacg tctccgccct
     4081 tgcggctcgc gacggggtga tcgattttgg agacttgcag gcggaacgga gctacaggcc
     4141 ctgggccgtc tactgccagt cgaagctcgc caatctcttg ttctcctttg acctccaacg
     4201 ccgaagtgat gcagcggggt ggggactgac gagcatagcc gcacacccag gcatttccag
     4261 aacagatctg gtcagcaacg ggtttgggaa gaacagcgtg gcctccatcc tgatgcgtct
     4321 ggtgggtgcg ttgtttttcc aagccgcatc ccagggtact cttccaactc tcttttccgc
     4381 aacctcgccg ctggcgaagc ctggagctta tcacggccca gatggtcgaa acgaaatgcg
     4441 aggcacgcct gccgcgtcga aggcgccacc ccaagccgag gatgcggacg tggcgaggcg
     4501 tttgtgggaa ttgtctgaac gactggttcg cgtgacattc gagtagaggc cctggctccc
     4561 gtggcggcgt ggcggggcag cgtggcgcca ggttctactg caggaacacc ttctccttga
     4621 agggcgccag gtacttgctg gacatcgtgg aaagcggctt caccgcaagc gcgaacttct
     4681 ccgccaaggt ccccatcacg ctactgagct ggcggacctc cgcgtccgga tgacgggagg
     4741 ccaggcgata gaacgagctc ccctgcgtga ggtaatcctg ccgggtcctc cgccgggaga
     4801 cgcgcaggct ctcccacggg acggcgccgg cggccgaatg actttccgca ggtcgcaccg
     4861 aggcggaccg aagcgggtcc gcctcctccg gcggcatgat ccccagcgtg aagagacaca
     4921 cgtccgccgc gcgcttgtac acggggaacc gtcgggcctc ttcgagggcg ctcgcgtagc
     4981 tcagcaggct gtcgaggtcg aagtcgctga accgcacgcg cctccacacc ccgtgcttca
     5041 cgcggaccac cgtggagaaa ctgctcacgc gaacaaagga caccaacatc tccgtgaggt
     5101 aggacaggag ctctccgcgg tccagaaggg ccacgatctg cggcgtgtcg aacagcgcca
     5161 ggctgcgccc gccggagtgc tccagggtga aacgctcctt ctccaggtcc gcgcggaccc
     5221 ggcggaggag cacggcgaag aggagggacg gggacacctg caggatggac tggggatcgt
     5281 ccaggaggcg gtgcgccagc ttggggtcct tgagcatccc ctccaggatc tcggggtcgt
     5341 cccgcaggac gcggatcatg cgggacgcgt cctgggtctg cggcaccagc gtcttcacca
     5401 gatagagcag gtcgctgtcg ctgaagatga aacggtaccc ctgcatgaac gcacgccctc
     5461 ccggcacgga gaatatacca acggccgcgc cgggacgacg aaccggcgct cttttcagtc
     5521 cggccgaatg gacctatgat cgaggcgagc gatcctcaag gaggcagcca tgctggaaaa
     5581 agaccagctc ggcggcggaa aggtccgggt cacgttcctg atgccggccc tggagggggt
     5641 cggcaccctc agcgtctgcg gtgagttcaa cgactggagc ccggccgccg cgccgatgac
     5701 ccgagcggcg gacggcacct ggagcgcggc gctcaccctg gaggccggcc gctcctacca
     5761 gttccgctac cgagacgacc gaggcggctg gcacaacgac ccggccgcgg atacgtacgt
     5821 tcccaacgat ttcgggagcg agaactcggt ggtggatctt cgagacaagc cgagtggtgc
     5881 gggggccggc accgcgccaa ggaagaccgc gccctcgaag gttgcgcctg cgaagcccgc
     5941 gccctcgaag gctgcgcttc agaagcccgc gccgaagaag accagcccca agaagccgcg
     6001 cggaaaaggt caataggccc ggacccgcag accagacagg gagcacgcat gctgttcacg
     6061 acactcgctc tgaggggaat ccccctgcgc aaccgaatcg tggtctcgcc gatgtgccag
     6121 tactcctgca cggacggcat ggccaccgac tggcacctcg tccacctggg aagccgcgcc
     6181 gtgggaggcg ccgccctggt catgacggag gccacggcgg tggaggcgcg gggccgcatc
     6241 agcgccgcgg atctcggcat ctgggacgat acgcacgttc cgcccctgga acgcattgcg
     6301 cacttcatcc acgctcaggg ggcgctggcg gggatgcagc ttgcccacgc ggggcggaag
     6361 gcatcggtga aggcgccctg gcagggcgga gcccccgtgg tggttgggga cggcggatgg
     6421 gaggtggtgg gacccagtgc catcccgttc gacgaggcgt ccccggtgcc ccatgtcctc
     6481 ggagtggagg aagtgcgcgg tgtggtgcgg gagttcgcca gggctgccag ccgggccctg
     6541 gtggcgggat tccaggcgct ggagatccac gcggcccacg gctatctcat ccacgagttt
     6601 ctctcgccga tctccaacac ccgttccgat gcctacggcg ggagcttcga caaccggatc
     6661 cggctcctgc gggagatcgt ggcagagatc cgaaagagga tgccggagag ctttcccctg
     6721 ctggtccgga tctccgccac ggactggacg accgggggat gggacgtgga gcaatccgtg
     6781 gagcttgccc gacgcctgaa ggaggaaggg gtggacgtca tcgattgctc gtcgggcggc
     6841 aacgtgaagg gcgtgaccac cgctatcggc cccggctacc agacgccgtt tgccgagcgg
     6901 gtgagacgag aggcccagat caagacggcg gcggtcggcc tcatcacctc cgcccagcag
     6961 gcggaccacg ttcttcggac cggccaggcg gacctggtgg tgatggcccg ccagctcctg
     7021 cgggacccct actggccgct tcgcgcggcc gccgagctcc gccaggagat cgcctggcca
     7081 aaccagtact tgcgcgccaa agcgtgatct gcgaggcggc tagtcgcggt acaggtccag
     7141 aacgtcttct cccgccatgg ccaccccgat cgggcgcagc gtatggagga cccggatggt
     7201 cccttgatgc gctgccagca cctcagggag ccgacggtag acgtgggggc tttcatcggt
     7261 tcccgccccg cgcagctcca cgccctcgcg ctccatccac tgcatcatca tctggcggct
     7321 gaccaggccc ggccggacca cctgctgcac acggcggccg cccttcttcg cccatcgacg
     7381 tttgcccgcc gcctgcgtgc ggctcatgag ccgacccgct ccgtgcaccg tgctgtgcag
     7441 cgcctcttcg gactccggcc cggccacgcc ttcgacgatg acgctgatgt cgcccatgga
     7501 gccgccgacg atgccccgct gcccgggcca cgccggcgtg gccccctttc gcacaacgac
     7561 caggcgctcc ccgtggtgct cctcgatcca ggcgaagttg tggtgattgt ggaccacgtc
     7621 caccggatgg gcgccgagaa gcgacaggac ctggtcaacc acgatgtccc gacccgcgta
     7681 cgcgtagcgt cccgcaaggg tcatggcgcg ccagtagtcc tgccccaacg ccccgtccag
     7741 cggaaggacc gtggccggct ggtccacgcc ctctcccggc gctcgcgcat cgaaggaccg
     7801 cccggcggcc aggttcagaa agccgcttgc cgtcttgtac ccgaagcccc ggcttccgaa
     7861 gtgggcggca acccacagcc gcccgtcctc gtcctcaagg agatccacga agtggttccc
     7921 ggatccgacc gtcccgagct gctcccgcgc caggcggcga agcggctcca gcgggcggac
     7981 ctcacgccag cctggatcgt cgaacacctc gtggtcgatg gacttcccgc tggagcgccc
     8041 aagaccgaag acgaccgtag cggcgacagc gtccatgatc cgcttgattt caggacgcac
     8101 ctgggaggcg gtcacctccg tgcgcaccgc cttcaacccg caggagatgt cgtagccgac
     8161 cccgctcgga gaaacggcat cccgatacgc caccacgccg ccgatcggca tggagtagcc
     8221 cttgtggccg tcggccatga gcgccgccgc ggctacgcga tcgtccgagg cgcatctgcg
     8281 gatctgggcg agggtggcct cgtcgtgcgt gccccagacg gggatcccgt tcaggagctg
     8341 gtagcccatc ggagccccct gcgccaggtc cgcgccggcc ccggaacctt tacagcttca
     8401 ggtcggggtt tccgggtccg aagtgtttca gcatcaccag cggatcggtc cgggacgggt
     8461 tggcgatctt cacaccggcc ttggcggcat cctcggtgac gaagaactcg tccatggtga
     8521 gctgaccgaa ccggatgacc gccgggctgt cgatgtccca aggccccatc gtgccgtgac
     8581 cctgcaggag gatcatgccg taggccgccg cgtccttgat ggtgacggtc ttccccggca
     8641 gcaccctgag ctccttggcg ctgaacgccg aggaccgata cgtgatccag ctctcctcgt
     8701 acccctccgc ccgcgtctgt tccacgggcc ggacgggcag cggccgcatg aagtggttgg
     8761 ccgtgaaagt ggggtccacg ttcgcctccc agtccaggag gctgatgagg aactcgatat
     8821 cgcccacccg gtctttgggg acgtccttcc agagcagctc ttcggggacg atggaatccc
     8881 cggtgaggga ctggaacatc gagaacacgt cggaggcctt ctgcggctcg tacgtgcaga
     8941 gggtgcctgg cgcgtgcagg atcccgcccg ccacgtccca gccggtccct ggctccaggc
     9001 ggtaggccgt ggacaggttg gtgatcttgt tgtcgccctt ggtgaagttg cgcaggcagt
     9061 cccgcacctg gtccttcgtg gtcccgggct tcagcccgaa gaaggtgtac gggaagtcgg
     9121 cgccatagtt gttgagctgg accgggaagt agtacgcctc gggcttcccg gtgcggccgg
     9181 caagcttcgc ctgctcgtcg cgatgatgga cgtgatgcgg cagggggccc ttgttgtcga
     9241 agaacttgga gaacatgggc cagccctggt gcgcgctcca gatccgctgg ccgatgatct
     9301 tctccttgag ctcggcaacc gcgtcccgaa ggagcacctt ctcggtcgtt ccgcgatcct
     9361 cgaagacgat gaaggagagg ccctcgttgg ggctcgtctt ggggccgttg tccgccggcg
     9421 tggtggaggc gaaccagcgc tcgtcgatgc ctccgcggtc gccgcccagg gcgtagtagt
     9481 cgtccggatg gagcttgatg cgtcgacctg gaacgcagaa cgaccggggc acccaggccg
     9541 gcgccagccg gagaatccct ttgccctgct cgagcgcgct ctcgacgatg gaccgtgtag
     9601 tcatcgtagc ctccctcgag ccgatgcgga accccggatc caggccccgg ggacatccct
     9661 ggctcggcgg agaaacggta gcagatcggc gcgcccgacg aaagggggag cgatggggcc
     9721 gcccggaggc tcttgacagc acgttcggct cccggtctca ttcctggcag cgaggaagac
     9781 caggcttcgc cgggcccatc cggactttcg ggcagcagcc aacgctgcgg gactctcgca
     9841 ttttcggggc cggcgggacg agcaatcgcc cgtgcgaacc cgaaccgcaa ggagttccgt
     9901 gatgaaccgg gccactcgca ccgcgcgtct gtccatcctg tcgaacacgc tcctcatcgc
     9961 gctgaagttc accgtcggcc tggtgagtgg atccgtgagc gtgctctccg aggccacgca
    10021 ctccctgatg gacctggtcg ccgccttcat ggcgttcttc tccatccgga tggcccaacg
    10081 cccggccgac ccgcagcatc cctacggcca cgagaagatc gagaacgtct ccggcgtgct
    10141 ggaggcgctg ctgatcctgg cggccgtctg tctcatcgtg tatgagtcgg tgaagaagct
    10201 gatcacggga gagccggccc agcgcctgga gctgggtctt ctcgtgatgg tggtctcggg
    10261 ggtggtcaac ttgttcgtct cccggcgtct ctaccgcgtg gcggccgaag aggg
//
LOCUS       DASRZS010000418         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1383542, whole genome shotgun sequence.
ACCESSION   DASRZS010000418 DASRZS010000000
VERSION     DASRZS010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383542"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="VFH83_10265"
     CDS             <1..1403
                     /locus_tag="VFH83_10265"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET6486794.1"
                     /translation="SSPHQLEGCKLISRQVKGPVIAALARAVDKDIECAAQSLQGAER
                     PRIHTFLATSPIHMEYKLRKKPDEVLEMAVKAVRLARNLTAEVEFSPEDATRSEIPFL
                     CRVIEAVIKAGATVINLPDTVGYTVPREFSAFLRKILEGTPNFDKAVLSVHCHNDLGL
                     AVANSVEAVQVGARQVEVTVNGIGERAGNASLEEFVMALNVRRDVLPYTTGINTRHIY
                     HTSRLLANVIGFPIPRNKPITGENAFAHESGIHQDGVLKNRETYEIMTPESVGRDSSQ
                     LVLGRHSGMHGFRDRLTQLGLSLTEEETRKAYDRFLEIADRKKEVYDDDLYVIVSDQL
                     GRETETYLLDYFNIQSGNLSVPTATVRILNGAERHEEAATGDGPVDAIFNAIDRTLGL
                     STKLLEYVVQAVTPGRQAMGEVSVALEIDQRKFVGRGASTDILEASAKAYVNALNRWR
                     AYQAVRSAGEGNGDTH"
     gene            1387..>2451
                     /locus_tag="VFH83_10270"
     CDS             1387..>2451
                     /locus_tag="VFH83_10270"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HET6486795.1"
                     /translation="MATLTEKILARAAGRETVRPGEFLEIEPDLGLANDITAPFAIEE
                     FEKTGAAGPRFPERVFLVADHFTPNKDIQSAEQVRVLREFSHKHPGVHFFEQGEVGVE
                     HALLPEMGAVVPGMVVIGADSHTCTYGALGAFSTGVGSTDMAGFYLTGKAWLRVPATI
                     RVEYTGALGPWMTGKDLVLALIGQVSVSGATYQALEVAGPPIDALSVEGRLTMANMAI
                     EAGAKNGIFAADATTAQYLKGRTDAAWEPLLPDPDTAYARTVRIDVSGLEPLVAFPHL
                     PQNVRPAAEARDRSVDQVVIGSCTNGRIEDLRLAARILRGRRVARGVRCIVIPATPRT
                     YRQALEEGLFEVFLDAGAVIS"
BASE COUNT          431 a          827 c          821 g          372 t
ORIGIN      
        1 tctcctcgcc gcaccagctc gagggctgca agctcatctc ccgacaggtg aaagggcccg
       61 tgatcgcggc ccttgcgcgc gccgtggaca aggacatcga gtgcgcggcc caatccctgc
      121 agggcgccga acgaccgcgc atccatacgt tcctggcgac ctcccccatc cacatggagt
      181 acaagcttcg caagaagccg gatgaggtgc tggagatggc cgtgaaggcg gtgcggctgg
      241 ccaggaacct gaccgcggag gtggagttct ctccggagga cgccacccgg agcgagatcc
      301 cgttcctctg ccgcgtcatc gaagcggtca tcaaggccgg cgccaccgtg atcaacctcc
      361 ccgacacggt ggggtacacg gtgccccggg agttcagcgc gttccttcgc aagatcctcg
      421 agggcacgcc caacttcgac aaggcggtgc tctcggtcca ctgccacaac gacctggggc
      481 tcgcggtcgc caactcggtg gaggcggtcc aggtcggcgc gcggcaggtc gaggtcacgg
      541 tcaacgggat cggggagcgg gccggcaacg cttcgctgga ggagttcgtc atggcgctca
      601 acgtgcgtcg ggacgtcctt ccctacacca cgggcatcaa cacccggcac atctaccaca
      661 cctcccgtct gctggccaac gtgatcggat tcccgatccc gcgcaacaag cccatcaccg
      721 gtgagaacgc ctttgcccat gagtccggca tccaccagga cggcgtgctc aagaaccggg
      781 agacctacga gatcatgaca ccggagtcgg tgggaagaga ctccagccag ctcgtgctgg
      841 gacggcattc cgggatgcac ggattccggg accgcctcac gcagctcggg ctgtccctca
      901 ccgaggagga gactcgcaag gcctacgatc ggttcctgga gatcgcggac cgcaagaaag
      961 aggtctatga cgatgacctg tacgtgatcg tctcagacca gctcggacgg gaaaccgaga
     1021 cctacctcct ggactacttc aacatccagt ccggcaacct ttccgtcccg accgccacgg
     1081 tgcgtatctt gaacggcgcc gagcgccacg aggaggccgc caccggggac ggtcccgtgg
     1141 acgccatctt caacgccatc gaccgaaccc tgggcctctc caccaagctc ctggagtacg
     1201 tggtccaggc ggtcacgccg ggccgccagg ccatgggaga ggtcagcgtg gccctggaga
     1261 tcgaccagcg gaagttcgtg ggccgcggcg cttccacgga catcctggag gccagcgcca
     1321 aggcgtacgt gaacgcgctc aaccggtggc gcgcctacca ggcggtgcgg tcggcgggag
     1381 agggaaatgg cgacactcac tgagaagatc ctggcccggg ccgctggcag ggaaacggtc
     1441 cgtcccggag agttcctgga aatcgagccg gacctggggt tggcaaacga catcacggcg
     1501 ccgttcgcca tcgaggagtt cgagaagacc ggtgccgccg ggccgcgctt ccccgagcgg
     1561 gtcttcctgg tggcggacca cttcaccccg aacaaggaca tccagtccgc ggagcaggtc
     1621 cgggtgctgc gggagttcag ccacaagcac cccggtgtcc acttcttcga gcagggcgag
     1681 gtcggcgtcg agcacgcgct cctgccggag atgggagccg tcgtgcccgg catggtggtg
     1741 atcggcgcgg actcccatac ctgcacctac ggggccctgg gcgccttctc caccggggtg
     1801 ggctccaccg acatggccgg tttctacctg acgggcaagg cgtggctgcg cgtgccggcc
     1861 accatccgtg tcgagtacac cggcgcgctt ggaccctgga tgaccggcaa ggatctggtc
     1921 ctggccctca tcgggcaggt gtcggtctcc ggagccacct accaggccct cgaggttgcc
     1981 gggcctccga tcgatgccct ctccgtggag ggacggctca ccatggccaa catggccatc
     2041 gaggccggcg ccaagaatgg catcttcgcg gcagatgcca cgacggctca gtacctcaag
     2101 ggccgaaccg acgcggcctg ggaaccgctg ctgccggatc cggataccgc gtacgcgcgc
     2161 acggtgcgga tcgacgtctc ggggcttgaa cccctggtcg cctttccgca cttgccgcag
     2221 aacgtccgac ccgccgcgga ggccagggac cggagcgtgg accaggtggt gatcggtagc
     2281 tgtacgaacg gccgcatcga ggacctgcgc ctggcggcgc ggatcctgcg cggcagacgc
     2341 gtggccaggg gggtacgctg catcgtcatc cccgccacgc cgcggacgta tcgacaggcg
     2401 ctggaggagg ggctgttcga ggtcttcctg gacgcgggag ccgtcatctc g
//
LOCUS       DASRZS010000419         4264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4458861, whole genome shotgun sequence.
ACCESSION   DASRZS010000419 DASRZS010000000
VERSION     DASRZS010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4458861"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFH83_10275"
     CDS             complement(<1..279)
                     /locus_tag="VFH83_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6486796.1"
                     /translation="MRLKDRVALVTGAGAGIGRAIAREMVKEGARVAICDIHEGKLQS
                     AAEELRGLGAEVRAVRADVGSAADVDRMFADVVGHFGALDILVNNAAIF"
     gene            complement(384..1655)
                     /locus_tag="VFH83_10280"
     CDS             complement(384..1655)
                     /locus_tag="VFH83_10280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6486797.1"
                     /translation="MNDSGVKLYPYRWVVLITFMLINVMVQVLWICYAPVATLAASSY
                     GVTRDNVDLLANLFMLIYIPMAFPAAWAIDTFGFKKAVGFGAILMGVFGLLRAVLPTS
                     YPAALVGTIGISIGQPFLMSAFTKLAAEWFPQKQRATITGIIFLAVFLGIGLGESLGP
                     TLVGRYGLGGMQLIYGVAAAVAVVLFLGLARAKPPTPASPAGEEVRALVLDSLKQILR
                     SKDVYLLALALFIGSGIVNGVFTLIDGLSQEKSFTTDQGVVLTALLLVGGIVGSVVVP
                     ALSDGLRRRKLVVMIAILVGVPSTLGMVFGTGYLLECVCFFAMGLCVTGATPVAYQYG
                     AEITHPAPEGTSNGIFALMVQASGLLIVLMDALRGVFHDSYLPSFLGLGVLLAVCGLL
                     LLSARESPKMGRGEAVPRPAAGKVAGTSVGT"
     gene            complement(1652..2131)
                     /locus_tag="VFH83_10285"
     CDS             complement(1652..2131)
                     /locus_tag="VFH83_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486798.1"
                     /translation="MLRRALLVTVLMGAAASLSFSEPLDVVLSINPIAKLGAVFTTDS
                     SVLIGNPTDPAALITAGLVTTLYGVPAAFLLWNLATHNPPNVKVWRTISFGTDLGVTL
                     TVLGVGSYTLVSDILNPPGGEDWAGLVGALLILLAIPMGLGTLADTVPFPMEAGGKP"
     gene            2251..4035
                     /locus_tag="VFH83_10290"
     CDS             2251..4035
                     /locus_tag="VFH83_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486799.1"
                     /translation="MHVRKSHVAVWIITAAILALLLLGPMTDAWPAALLLAASAHFLL
                     NLIFSARTRLSVASRLDRPVMLDHPESSPAALRESFAEEKRDSDLRLPVRLDGRVKGK
                     NTERSPWSDRPCVGWGLSVEIYRKTTLGGDADYALLERRYGLGEFTLQQDDEGIRVAP
                     PGIVRGPHEHESLIAWDGVDKAPKLKDIVDNAMQVQKLSRKRFTGVRVAEALLEDGDR
                     VTVWGTAAKEGDSVVVKGSATEGDPGALLAASLSTRARARGAGRGTLRIGLNALLTVA
                     ALGLGVLVGTGAPWWKLEKAYPWVNVEQVGTLSWTGNGKSYEVSGKTLNLGGTSSWSL
                     DPSDTQVSLVSHDVDAVVKGSTEITLTRPLRSSFTVYPAAPAYPRYLRSQFRFTVPPV
                     SPAVQELSTHQGPFYVVNRTAGEVEVRFRTASTRKALQGKYWSIDPAEKTYLVVSDQR
                     FNLTEGDAALVRRAGESSERDAWVTLGGGGAATWETTGRRWVLTVDEAALIGREGPLV
                     TSNPAQYEVQLEVFDRGGESSLGTWTLASGFGGPQGEALESGGSPFVFKDGYRISVEP
                     HANDLVYRGPLNGYPKATFKDGVWSIGP"
BASE COUNT          779 a         1396 c         1447 g          642 t
ORIGIN      
        1 gaagatggcc gcgttgttga ccaggatgtc gagcgccccg aagtgaccca ccacgtcggc
       61 gaacatccga tcgacgtccg ccgcggagcc cacgtccgcc cgtaccgcgc gcacctcggc
      121 ccccaggccg cgaagctcct cggccgcgga ctggagcttg ccctcgtgga tgtcgcagat
      181 cgccacccgg gcgccttcct tgaccatctc ccgagcaatg gcgcggccta tgccggctcc
      241 cgcccccgtg accaatgcga cccggtcttt cagcctcatg ggtgcctccc gagacggacc
      301 tgaggatcag tatggagcaa acgcggcgcc gggacaataa gcgcccacgc gccggcgccg
      361 cgcccgcggc gtgccgaaag gcgtcaggtg cccacgctcg tccccgcgac cttgcccgcg
      421 gccggccggg ggacggcctc tcccctcccc atcttgggcg actcccgggc gctcaggagc
      481 aggaggccgc agacggccag aaggacgccc aggcccagga aggacggcag ataggaatcg
      541 tggaacaccc cgcgaagcgc gtccatcaga acgatcagca ggcccgatgc ctgcaccatc
      601 agggcgaaaa tgccgttgct ggtcccctcc ggtgccggat gagtgatctc agcgccgtac
      661 tggtaggcca ccggcgtggc accggtcacg cagagcccca tcgcgaagaa gcagacgcac
      721 tccagcaggt aaccggtccc gaagaccatg cccagcgtgg acggcacgcc gaccaggatg
      781 gcgatcatga ccacgagctt tcggcgacga agaccgtcgg agagggcggg taccacgacg
      841 ctgcccacga tgccgcccac gaggagcagg gcggtcagca cgactccctg gtccgtggtg
      901 aaggacttct cctggctgag cccgtcgatc agcgtgaaca caccgttgac gatcccgctt
      961 ccgatgaaga gggcgagggc caggaggtag acatccttgc ttctcaatat ctgtttgagg
     1021 ctgtccagca cgagcgcccg gacctcctct cccgccggac tggccggcgt gggaggcttg
     1081 gcccgcgcca ggcccaggaa caggactaca gcgacggcag cggcgacgcc gtagatgagc
     1141 tgcatgccgc ccaggccgta acgtcccacc agggtgggcc ccagggactc gcccagaccg
     1201 atgcccagga acacggccag aaagatgatc cccgttatgg tggcccgttg tttctgcggg
     1261 aaccactccg cggcgagctt ggtgaacgcg ctcatgagga agggctggcc aatggagatc
     1321 ccaatggtcc cgacgagggc cgccggatag ctggtgggaa ggaccgcccg caggagcccg
     1381 aagaccccca tcaggatggc cccgaacccg accgccttct tgaacccgaa ggtatcgatg
     1441 gcccatgccg ccgggaacgc catggggatg tagatcagca tgaagaggtt ggccaggaga
     1501 tcgacgttgt ctctcgtgac gccgtaggag gaagccgcga gcgtggccac cggcgcgtag
     1561 cagatccaga gaacctggac catcacgttg atgagcatga acgtgatgag gactacccaa
     1621 cggtagggat agagcttcac gcccgagtca ttcatggctt tcctcctgct tccatgggaa
     1681 acggtaccgt gtcggcgagc gtaccaagcc ccatgggaat tgccaacagg atcagcaagg
     1741 caccgacgag acccgcccag tcctcgccgc ctggaggatt caggatgtcg cttaccaggg
     1801 tgtaggagcc aacccccagg acggtgaggg taacccccag gtccgtgccg aaggagatgg
     1861 tccgccacac cttgacgttg ggggggttgt gggtggccag gttccagagg agaaacgcgg
     1921 cagggacgcc atagagcgtg gtgaccaggc cggcggtgat cagggcggct ggatccgtgg
     1981 gattgccgat cagcacgctg ctgtcggtcg tgaacaccgc gccgagcttg gcgatgggat
     2041 tgatgctgag gacgacgtcc aagggctcgg agaacgaaag gctcgcggca gcccccatga
     2101 ggaccgtcac gagcaaggct cgtctgagca ttctgaagtc tcctgttgct tgcccaaaag
     2161 taaccacaac ggcacgggaa ggacaccccg gagcggcaac ggcccaggga acggttgacc
     2221 ccgacggacc tccggcccca tagtccatcg gtgcacgtac gcaagtccca cgtggccgtc
     2281 tggatcatca ccgcggccat cctggccctc ttgctgctgg ggcccatgac cgatgcgtgg
     2341 cccgccgcgc tcctcctggc cgcgagcgcg cacttcctcc tcaacctgat cttttccgcc
     2401 cggactcggt tgagcgttgc cagtcgactg gatcgcccgg tcatgctgga ccatccggag
     2461 agctccccgg cggccctgcg ggagagcttt gccgaggaga agcgggacag cgacctccgt
     2521 ctgcccgtcc gcctggacgg ccgcgtcaag ggaaagaaca cggagcgctc cccctggagc
     2581 gatcgtccct gtgtgggatg gggcctctcg gtggagatct accgcaagac cacgctgggc
     2641 ggagatgcag actacgccct cctggagcgg cgctacggtc tcggtgagtt cacgctccag
     2701 caggatgacg agggcattcg cgtggcgccg ccgggcatcg tgcggggtcc ccacgagcac
     2761 gagtccctca tcgcctggga tggcgtggac aaggctccaa agctgaagga catcgtggac
     2821 aacgccatgc aggtccagaa gctctcccgc aagcggttca cgggagtccg ggtggcggag
     2881 gcgctcctgg aggacgggga ccgggtgacc gtgtggggaa cggcggccaa ggaaggggac
     2941 tccgtcgtgg tcaaaggcag cgccacggag ggcgatcccg gggcgcttct ggctgcttcc
     3001 ctctccacgc gagcgcgcgc ccggggcgcg ggacggggca ccctgcggat cggcctcaac
     3061 gcgctcctta cggtggcggc gctgggtctg ggagtcctgg tcgggacggg cgcaccctgg
     3121 tggaagctgg agaaggcgta tccctgggtc aacgtggagc aggtcggaac gttgtcctgg
     3181 accggcaacg ggaagagcta cgaggtttcc ggcaagacgc tcaacctggg cggcacctcc
     3241 agttggtccc tggatccctc cgacacgcag gtgtctctgg tttcccacga cgtggacgcc
     3301 gtggtgaaag gctccaccga gatcacgctc acccgacccc ttcggtcctc attcaccgtc
     3361 tacccggccg cccccgccta tccacgttat ctgcgctccc agttccggtt caccgttccc
     3421 cccgttagcc cggccgtcca ggagctctcc acacaccagg ggcccttcta cgtggtcaac
     3481 aggaccgcgg gagaggtcga ggtccggttc cgcaccgcat ccacccgcaa ggctctccag
     3541 ggcaagtact ggtccatcga ccccgcggag aagacctatc tcgtggtaag tgatcagcgg
     3601 ttcaacctca ccgaaggaga cgcggcgctg gtccgccgcg ccggagaaag ctcggagagg
     3661 gacgcctggg tgacgctggg cggaggcggg gcggcaactt gggagactac cggccggcgc
     3721 tgggtgctca ccgtggatga ggcggccctg atcgggagag aggggccgct ggtgaccagc
     3781 aatccggcgc aatacgaggt ccagctcgag gtcttcgacc ggggcggaga gagctcgttg
     3841 ggtacctgga cccttgcctc cgggttcggt ggaccgcagg gagaagcgct ggagtccggc
     3901 ggctcgccgt tcgtgttcaa ggacggatac cgtatctcgg tggagcctca cgcgaacgat
     3961 ctggtgtacc gtggtcctct gaacggatac cccaaggcga cgttcaagga cggggtctgg
     4021 agcatcggac cgtaggaaga cggccgaagg tccgacggtg gtcgccgccg cagcgcggtt
     4081 ggcggaccag gatcagcgcc gcaggaaggg gctcgcggcg agcacccgcc gaaactcgtc
     4141 ctcggcggac accactccgt agtggtcgtg cgagcttcga aggaacgcca cgcgcggacc
     4201 cagggatgcg tcgtcctggg taggatcgtc ccaggcttcc gtgagcggta tcccgtcgat
     4261 gcga
//
LOCUS       DASRZS010000420         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1762260, whole genome shotgun sequence.
ACCESSION   DASRZS010000420 DASRZS010000000
VERSION     DASRZS010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762260"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFH83_10295"
     CDS             complement(<1..896)
                     /locus_tag="VFH83_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486800.1"
                     /translation="MGVSTKDRDTLRHLADKVALIGALPQQRETIKHWKALNGLRPVR
                     PMVTIDQVCWNEMDVDGELELHCETDFTRGIEQGLRRKIYAWRHMPVDMVVDPWVEIP
                     KVIRNDGYGIQVDERVAVLDPTNDVVGHLYTDQLKTEMDAMRIRAPRVSLDQEATARA
                     EETAREIFDGVLPVHLQGVFPAFNPWDQITTWRGAQNVLLDLVDRPALSHMIISRCTD
                     ALLAELDQLESQGLLGRDMGLVHCTGAFSDEIPAADYDPERPRARDLWTFGMAQIFGS
                     VSPAMHEEFDIQYARRWYARFGL"
     gene            complement(972..>2035)
                     /locus_tag="VFH83_10300"
     CDS             complement(972..>2035)
                     /locus_tag="VFH83_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6486801.1"
                     /translation="TLSQVSLENDFVPDAGLMKGTGITPAQIAAALQTYSSGQQASNV
                     ITGGLSYPIQVQADPTTLSSGQSLLNLPIYSSSLQTTMQIGQLGRFVLSEAPLSLSRY
                     NRQYTGELTLNLKQNAPTALTMQTMVTDQLTKAGLIGGSVALLPGGRFSQAALAAQLA
                     TSGPLIFLLALFLAYLVMAAQFNSWRYPIYLLLPVPLALVGALWFVYIIGGGLDIFGL
                     MGMLMLIGLSAKNAILYLDFVLQRIGKMPFADALIESARLRFRPIIMTTLTVLVISFP
                     LIFSHGQGSEFGQRMGIVMFGGVVFSAILTFFVVPAAFYLFERNRAANRAEAGPEDEP
                     MLQPPSEIPEGRVAVAGEG"
BASE COUNT          349 a          650 c          705 g          331 t
ORIGIN      
        1 aggccaaacc gggcgtacca gcgccgcgcg tactggatgt cgaactcctc gtgcatggcc
       61 ggggacacgg agccgaaaat ctgcgccatc ccgaaggtcc agaggtcccg ggcccggggc
      121 cgttcggggt cgtagtccgc ggcggggatc tcgtcgctga acgcgccggt gcaatgcacc
      181 aaccccatgt cacgccccag gaggccctgg gattcgagct ggtcgagctc cgccagcagg
      241 gcatccgtgc accgggagat gatcatgtgg ctcagcgcgg ggcgatccac caggtccagg
      301 aggacgttct gcgctccccg ccacgtggtg atctggtccc aggggttgaa ggccggaaac
      361 acgccctgca ggtgcacggg caggacacca tcgaagatct cccgcgcggt ctcctcggct
      421 cgagcggtgg cctcctggtc cagggatacg cgaggcgccc ggatcctcat cgcatccatc
      481 tcggtcttga gctgatcggt gtagaggtgc ccgaccacgt cgttggtggg gtccagcacg
      541 gcaacccgct cgtccacctg gatcccgtag ccgtcgtttc ggatgacctt cgggatctcc
      601 acccacgggt ccacaaccat gtcgaccggc atgtgccgcc aggcgtagat cttgcggcga
      661 aggccctgct cgatcccgcg cgtgaagtcg gtctcacagt gcagctcgag ctcgccatcg
      721 acgtccatct cgttccagca gacctggtcg atggtcacca tgggccggac cggccgcagg
      781 ccgttcaacg ccttccagtg cttgatggtc tcccgctgct gcggcagggc gccgatcagg
      841 gctaccttgt cggcgagatg acggagtgta tcgcgatcct tcgtgctgac gcccatggcg
      901 gcacctccgc ggccattatg ccgctccggg tgccgccagg actatagggt gggaccgcgc
      961 ttcccggcgg ctcagccctc tcccgccacg gccacgcgac cctcggggat ctccgagggc
     1021 ggctgcagca tcggctcgtc ctccgggccc gcctccgcgc ggttggccgc gcggttgcgc
     1081 tcgaagagat agaacgcggc cggaaccacg aagaaggtga ggatggcgga aaacacgact
     1141 ccgccgaaca tcacgatccc catgcgctgg ccgaactcgg atccctgacc gtggctgaag
     1201 atcagcggga agctgatgac gaggaccgtc agggtggtca tgatgatggg tcggaaccgc
     1261 agccgggcgg actcgatcag ggcgtccgcg aacggcatct tgccgatgcg ctgcagcacg
     1321 aagtccaggt acaggatggc gttcttggcg gatagcccga tgagcatcag catccccatg
     1381 aggccgaaga tgtccaaccc tcccccgatg atgtagacga accagagggc ccccaccagt
     1441 gccagaggca cgggcaagag caggtagatg gggtagcgcc aggagttgaa ctgggcagcc
     1501 atgaccaggt aggccaggaa gagcgccagg aggaagatca agggtcccga ggtggcaagc
     1561 tgcgcggcca gggccgcctg gctgaaccgc ccgccgggga gcagggcaac cgagccgcca
     1621 atgagtcccg ccttggtgag ctggtccgtg accatggtct gcatggtcag ggcggtgggc
     1681 gcgttctgct tcaggttcag ggtcagctcc cccgtgtact gtcggttgta tcggctgagg
     1741 ctgagcggcg cctcgctcag gacgaacctc ccaagctgcc cgatctgcat ggtggtctgc
     1801 agcgacgagg agtagatggg gaggttgagc agggactggc cgctggacaa cgtggtcggg
     1861 tccgcctgca cctggatggg gtagctcaga cctcccgtga tcacgttgga ggcctgctgc
     1921 cccgaggagt aggtctggag cgccgccgcg atctgggccg gcgtgatgcc ggtgcccttc
     1981 atgagcccgg cgtccgggac gaagtcgttc tcgagggaga cctgggacag ggtcg
//
LOCUS       DASRZS010000421         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3687182, whole genome shotgun sequence.
ACCESSION   DASRZS010000421 DASRZS010000000
VERSION     DASRZS010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3687182"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFH83_10305"
     CDS             complement(<1..252)
                     /locus_tag="VFH83_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET6486802.1"
                     /translation="MGALIQRVGDDLRVPDVPLIPFIEGDGTGPDIWRAARRVFDEAV
                     RRAYAGRRNIEWVQVLAGERAFQETGSWLPKETEEAFRSH"
     gene            323..1369
                     /locus_tag="VFH83_10310"
     CDS             323..1369
                     /locus_tag="VFH83_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486803.1"
                     /translation="MLGRFFARLALGVAALAFLSACAVSDTRFYLGAFQQNADLRELF
                     HLFDKEKDQENRFVLIQQIASTLENSGQRDKEILFLTTHVEKNPADIYNGYYLVLVAD
                     TYRDLRQAPLATHYYRRVLANNVDLLVEGRSIHLHCLQELLNLDPNPENKIEYYKELI
                     SRWPDQNAGSNWYYMAKAYEQVGEWEQAIQAYVKFTYSADTDIPGEPRAYRDAQEKIG
                     YYSPGVDKTWMLPDLQTLAAAVRDAITTKNVSKLVKYEAKVNFFQEPWDQTTLLDEQV
                     SYNIAQYLPISGVRVEGDLDIAANDREAYLKTTGWNFRPPTWYLYFRRIDFPANPDIN
                     GQWEWAGVYFGEKL"
     gene            1369..>2101
                     /locus_tag="VFH83_10315"
     CDS             1369..>2101
                     /locus_tag="VFH83_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET6486804.1"
                     /translation="MIIASISAALATDCQGTAPVVSPAPEALPAVATLPPPVMPVRDA
                     TIEEPGPDATSSPSPQQGLPPVNEPLAPLQGLRAEELYSLEMPDQAVVSAYLIGLLTS
                     QREWLQAALENSIPYRLAISRAIAERGLPREIQFLPAVESGFQPRAVSPQGATGLWQL
                     MRNTASPYGLRMDPWIDERKDFLKATAASLQKLQENYDQFGDWYLALAAYNCGAGRLS
                     AILRRYPHADFWTLRRKAALPRETAA"
BASE COUNT          388 a          720 c          648 g          345 t
ORIGIN      
        1 gtggctgcgg aaggcctctt ccgtctcctt gggcagccag gagccggtct cctggaacgc
       61 ccgctcgccg gcgagcacct ggacccattc gatgttgcgc cgacctgcgt acgcgcggcg
      121 cacggcctcg tcgaaaaccc gacgcgccgc cctccatata tcaggaccgg tcccatcgcc
      181 ctcgatgaag gggatgaggg gaacgtccgg gactcgaagg tcgtctccga ccctctggat
      241 cagtgctccc atggaagggt catggtacag gaaaccctgc gacttgccaa ccgctgccca
      301 tcctgatagt atccccgaac caatgttggg acgattcttc gcgcggctgg ccctgggcgt
      361 cgccgccctg gctttcctgt ccgcgtgcgc cgtttccgat acccgattct acctgggagc
      421 ctttcagcag aacgccgatc ttcgcgagct gtttcacctc ttcgacaagg agaaggacca
      481 ggagaaccgc ttcgtcctga tccagcagat cgcctccacg ctggagaact ccggccagcg
      541 cgacaaggag atcctcttcc tcaccaccca cgtggaaaag aacccggcgg acatctacaa
      601 cggctactac ctggtgctgg tggcggatac ctaccgcgat ctgaggcagg cccccctggc
      661 cacgcactac taccgtcggg tcctggcgaa caacgtggac ctcctggtgg agggcaggtc
      721 catccatctg cactgcctgc aggagctcct caacctggac cccaatcccg agaacaagat
      781 cgagtactac aaggagctga tctcccggtg gccggaccag aacgccggct ccaactggta
      841 ctacatggcg aaggcctacg agcaggtggg ggagtgggag caggccatcc aggcgtacgt
      901 caagttcacc tactccgcgg acaccgatat ccccggagag ccgagggctt accgcgacgc
      961 gcaggagaag attggctact attcaccggg cgtggacaag acctggatgc ttccggacct
     1021 ccagaccctg gcggccgcgg ttcgggacgc catcaccacg aagaacgtct cgaagctggt
     1081 gaagtacgag gcgaaggtga acttcttcca ggagccctgg gaccagacca cgctcctgga
     1141 cgagcaggtg agctacaaca tcgcgcagta tctgccgatc tccggcgtgc gggtggaggg
     1201 agacctcgac atcgcggcca acgatcgcga ggcctatctg aaaacgacgg gctggaattt
     1261 ccgacccccc acgtggtacc tgtacttccg tcggatcgat tttcccgcaa acccggatat
     1321 aaatggtcaa tgggaatggg caggagtcta cttcggcgaa aaactctagt gatcatcgcg
     1381 tcgatctcgg ccgcgctcgc aacggactgc caggggaccg ccccggtggt ctctcccgcg
     1441 cccgaagcgc tgccggccgt ggctaccttg ccgccgccgg tgatgccggt gcgggacgcc
     1501 accatcgagg agcccggacc ggacgccacc tcctcgccgt ccccgcagca gggccttccg
     1561 ccggtcaacg agccgctcgc gccgttgcaa ggcctgcgcg ccgaggagct gtactccctg
     1621 gagatgccgg accaggcggt cgtgagcgcc tacctgatcg ggctcctgac cagccagcgg
     1681 gagtggcttc aggcggcgct ggagaacagc atcccgtatc ggctggccat ctcacgcgca
     1741 atcgcggagc gcggcctgcc ccgagagatc cagttcctgc cggccgttga gtccgggttc
     1801 cagccgcgcg ccgtctcccc gcagggggcc accggcctct ggcagctcat gcgcaacacc
     1861 gcctcgccgt acgggcttcg gatggatccg tggatcgatg agcgcaagga cttcctgaag
     1921 gccaccgcgg cgtccctcca gaagctccag gagaactacg accagttcgg cgactggtac
     1981 ctggccctgg cggcgtacaa ctgcggggcc gggcgcttgt ccgccatcct gaggcgatac
     2041 ccgcacgcgg acttctggac cctgagacgc aaggccgcgc tcccccggga gaccgcggcg
     2101 t
//
LOCUS       DASRZS010000422         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1762739, whole genome shotgun sequence.
ACCESSION   DASRZS010000422 DASRZS010000000
VERSION     DASRZS010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762739"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="VFH83_10320"
     CDS             <1..1405
                     /locus_tag="VFH83_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486805.1"
                     /translation="VIGENLRFALGDFWAEFRKNRAGMVGLALVILFLAAVILEPRII
                     PFPKAGATWHDITAWDDNPPSAPPQWINRFTEKKAAITSVVTQYEKTSEQSDSSTLVT
                     YTFSYQDPYDVPPLDLIVHFVAAGDVPFAVSVVRPDREEVPLYQEQRQAGTREDVRVS
                     MDRNSASALFDYLKGYESEDTLSSVDPGQLKPVQILFSVAEPGMLKHPRPLKGMYQVK
                     LQGLLLSDASALDNPYVVFTGRVSGLLGTDNSKRDLWSGLVAGVKWALLIGVLTSFIA
                     VAVGVVYGIVEAYFGGLVNAILQRIWEVMVNMPLLPILIVLSAVFKPSIWFLIVIMSA
                     FFWVGPVKTVYSMALQIKEETYIEATRALGAGHGRIIFRHMVPILVPYSFASMALSVP
                     AAVVYEATVSLLGLGDASIVTWGQILHDAFSGGAVLNGLWWWVVPPGLMIAVMGMTFS
                     FVGFAMDTILHPKLRTR"
     gene            1402..2442
                     /locus_tag="VFH83_10325"
     CDS             1402..2442
                     /locus_tag="VFH83_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486806.1"
                     /translation="MSQESTARGDANVVEVRNLRLSYFTSKGPVQAVNGVSFDLKRGE
                     TLGVVGESGCGKTTLGLALLNIPTPPGRIVGGEIRIDGTDIVGLAERDLRRNVRWEKI
                     SMVFQGAMNCLTPVYTVGRQMTETLQKHRPTAREEAQRIVARYLGMVGLPTETVHRYP
                     HELSGGMKQRVVIAMALFLEPRVVILDEPTTALDVIVQAQIINVVKDLKRRLGLSMIF
                     ITHDLATEAEVADRILVMYAGKVAEIASNESVFGADVGVGSVPTGPAAARLSNHPYTR
                     RLLAATPRLHATVSELAFIPGTPPDLLEPPRGCPFHLRCSVAFDRCRTQEPPLREIGP
                     GHFAACWRCYDA"
     gene            2435..>2994
                     /locus_tag="VFH83_10330"
     CDS             2435..>2994
                     /locus_tag="VFH83_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6486807.1"
                     /translation="MPEAGLLTVTDLARHFPVRRSMGELLQGQRRMVRAVDGVSFSIR
                     KGEIFGLIGESGCGKTTTGKLVMKLLEPSSGSMSFMGRSSTRLDRQALARYRTCVQMI
                     FQDPYASMNPRFKIRDVLEEPLLIHHVGARRTERDERIRQALEAVKLTPVEDFMGRFP
                     HMLSGGQRQRAATARTLILAPALLVA"
BASE COUNT          488 a          988 c          989 g          529 t
ORIGIN      
        1 cgtgatcggt gagaaccttc gcttcgccct cggcgacttc tgggcggagt ttcgcaagaa
       61 ccgggccggc atggtcggtc tggccctggt gatcctcttt ctggccgcgg tgatcctgga
      121 gcctcggatc atcccgttcc cgaaagccgg ggcgacctgg cacgacataa cggcctggga
      181 cgacaacccg ccgtccgctc caccgcagtg gatcaaccgg ttcaccgaga agaaagcggc
      241 gatcacgtcc gtggtcaccc agtacgaaaa gacctcggag caatcggaca gctccacgct
      301 ggtgacgtac accttctcct accaggaccc ctacgacgtt cccccgctgg acctgatcgt
      361 gcacttcgtc gccgcggggg atgtgccgtt cgccgtgagc gtggtgcgcc cggaccgcga
      421 agaggttccg ctgtaccagg agcagcgcca ggcggggacc cgagaggacg tacgtgtctc
      481 catggatcgc aacagcgcca gcgcgctgtt cgactacctc aaagggtatg agagcgagga
      541 caccctctcc tccgtggacc cgggacagct caagcccgtg cagatcctct tctccgttgc
      601 cgagcccggc atgttgaagc acccccggcc cttgaagggc atgtaccagg tcaagctcca
      661 gggactgctc ctgagcgacg cctccgcgct ggacaacccg tatgtcgtct tcacgggccg
      721 cgtctccggc ctcctgggga ccgacaactc caagcgggac ctgtggagcg ggctggtggc
      781 cggcgtgaag tgggcgctcc tcatcggggt gctcacctcc ttcatcgcgg tcgctgtcgg
      841 cgtggtgtac gggatcgtgg aagcctactt cggcgggctg gtcaatgcca tcctgcagcg
      901 aatctgggag gtcatggtga acatgcccct tctcccgatc ctcatcgtgc tctccgccgt
      961 cttcaagccg agcatctggt tcctgatcgt catcatgagc gccttcttct gggtggggcc
     1021 ggtgaagacc gtctattcga tggcgctgca gatcaaggag gagacgtaca tcgaggccac
     1081 ccgggctctg ggcgccggtc acggccggat catcttccgg cacatggtcc ccatcctggt
     1141 gccctactcc ttcgcctcga tggcgctctc ggtgccggcg gcggtggtct acgaggctac
     1201 cgtcagcctc ctcggtctgg gagatgcctc catcgtcacc tggggccaga tcctgcacga
     1261 cgcgttctcc ggcggagcgg tgctgaacgg cctgtggtgg tgggtggtgc cgccgggcct
     1321 gatgattgcc gtcatgggga tgaccttctc cttcgtgggc ttcgccatgg acacgattct
     1381 ccaccccaag ctgaggaccc ggtgagccag gagtcgacgg cgcgcgggga cgccaacgtg
     1441 gtggaggtcc ggaatctccg gctcagctac ttcaccagca agggtccggt ccaggccgtc
     1501 aacggcgtga gcttcgacct gaagcgggga gagacgctcg gggtcgtggg ggaatccgga
     1561 tgcggcaaga cgacgctggg ccttgcgctt ctcaacatcc ctacaccgcc cggacgcatc
     1621 gtggggggcg agatccggat cgacggcacc gacatcgtgg ggcttgccga acgggacctg
     1681 cgaaggaacg tccgttggga gaagatctcc atggtcttcc agggcgccat gaactgtctg
     1741 accccggtct atacggtggg acgacagatg accgagacgc tccagaagca tcgacccacc
     1801 gctcgggagg aagctcagcg catcgttgcc aggtacctgg ggatggtggg gctgccgacg
     1861 gagacggttc accgttatcc ccacgagctg tccggcggga tgaaacagcg tgtcgtcatc
     1921 gccatggcgc tcttcctgga gccgcgcgtg gtgatcctgg acgagccgac caccgccctg
     1981 gatgtcatcg tccaggcgca gatcatcaac gtggtgaagg acctgaaacg ccgcctgggc
     2041 ctctccatga tcttcatcac ccacgacctg gcgacggagg cggaggtggc ggaccgcatc
     2101 ctggtgatgt acgccgggaa ggtggcggag atcgcctcca acgaatcggt gttcggggca
     2161 gacgtcgggg tcgggtctgt tccgaccgga ccggccgcgg cgcggctctc caaccatccc
     2221 tacacgcggc gcctcctggc cgcgactcct cggctgcacg ccacggtgag cgagctggcc
     2281 ttcatcccgg gcacgccgcc ggatctgctg gagcccccgc ggggatgtcc gttccacctc
     2341 cgatgcagcg tggctttcga tcgatgccgg acgcaggagc ctccccttcg ggagatcggg
     2401 ccggggcact tcgcggcatg ctggagatgc tacgatgcct gaggccgggc tcctgaccgt
     2461 cacggacctg gcccggcatt tcccggtccg tcggtccatg ggagagctgc tccagggcca
     2521 gcggcggatg gtacgcgccg tggacggggt gagcttctcc atccggaaag gggagatttt
     2581 cggcctcatc ggagagtccg gctgcgggaa gaccaccacc gggaagctcg tgatgaagct
     2641 cctggagccg tccagcggct ccatgagctt catgggacgg agctctaccc gcctggaccg
     2701 ccaggcgctg gcccggtatc ggacctgtgt gcagatgatc ttccaggatc cctacgcctc
     2761 catgaaccct cggttcaaga tccgcgacgt cctggaggag ccgctcctga tccaccacgt
     2821 cggcgcgcgg cgcaccgagc gggacgaaag gatccgccag gcgctggagg cggtgaagct
     2881 tacaccggtg gaagacttca tgggccgatt cccccacatg ctctccgggg gccagcgcca
     2941 gcgcgcagcc accgcccgca cgctcatcct cgccccggcg ctcctggtgg cgga
//
LOCUS       DASRZS010000423         7156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1000808, whole genome shotgun sequence.
ACCESSION   DASRZS010000423 DASRZS010000000
VERSION     DASRZS010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000808"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VFH83_10335"
     CDS             complement(<1..840)
                     /locus_tag="VFH83_10335"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486808.1"
                     /translation="MSTRINKPGRAAIVGAIGLTILVITGLSGCQQLLSILGLSHTLT
                     VTVSPSEAGYITRVPDLTNYSNGDKVQVTATANSGYTFSNWSGDASGTANPVTLTMDA
                     DKLVTAVFTAASASGKSLTAFSFPTIGATGVINQTAHTIAVTVPFGTNVTSLVATFTT
                     TGAQVKIGATVQASGVTPNDFTNPVSYTVVAADASTQAYLVTVSAASASDKRLTSFGF
                     VSPAVFGVIDQTGNTISVTVPSDTDVTHLVAEFVSTGALVLVGATIQASGTTTNDFSQ
                     PVTY"
     gene            complement(858..3185)
                     /locus_tag="VFH83_10340"
     CDS             complement(858..3185)
                     /locus_tag="VFH83_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HET6486809.1"
                     /translation="MRHSSWAKRVPLVPPVLVLVLLAMACSFPLSNPSASASLAAPGT
                     LQVTPSTVYIGLSWSPVSGAESYRVYRSSDGGPYSLVAQGVTALAWKDSGVTSGVSYS
                     YKVRAYRAAGGEGAPSAAVSARTADGAEAEKLVAAGKAYLSQRPPDYTRALTAFSRSL
                     QSDPTNYTALLWTCLLTLAQTITTSSVQTLATTYLGFENYPTSMDAFFQPGPHGVPAW
                     MGQAVDAGAMHFVYVGPNNGDWVSTGPNSYMSVEPGTGDYAFESYLSYVPQVRIPATI
                     QAWAGNPAVVTPGEYSMAVLYNLATHNPNGFNSLADHVVNGILSTALDQVVNTLEASS
                     LPDDAQIDITSDMFGGAFSSGWPLDVSSQPATISIGKAELLGLTGFLQMMKADLLMAK
                     TVSLDFPVQQYFNTLNPVDNPELNTTSLILAALYTLPNPIQGGFLCARPDAATTLAAA
                     KTAYVAALSSVDQAVTMILDRGEGSPFTISEFSSIPDISANWQTASDVLNYSHIAITK
                     MSDSVQNGHVMVIPTNFGSFPGPMEFIQYYATESNWPATPDDGPYSGPPQSIGVNLAP
                     AFVTPLAALDSLLDLRSNGEPNWYPFSLTFSAGTQQGGSPVLIDSAITDSDYAPLSGI
                     GAPEVVAALSQGTSIGAIAANLGAYHFYAIRLKDVTLGGTLAIDDTALGNLKDVIAAA
                     TSGLLSKTALDGLLVRNPTTGAITAYLPAFPAFVADNSFVAAGTAIAVDGNGDLAPDI
                     SYTTTGSFWWAVADMMQNANGPYVLGVDFAPVLPQ"
     gene            complement(3327..4646)
                     /locus_tag="VFH83_10345"
     CDS             complement(3327..4646)
                     /locus_tag="VFH83_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486810.1"
                     /translation="MTGRRASLGVALGIALGLVPLQAGAQAVAGGELNLGVSALVDPS
                     QPLASSIDDAIVFYPTIHVEDPAWRLDGKLSLEWDRAAVAPSLGIRDLYIELSPAGFL
                     TLRLGRFVYAPGSAGVLSPTHWFVTPDLLALVRNDTESLVRGGDLVQGILTLGDWSLT
                     LSASPFAPAWSIPDPSSPWFPKKDFPRNLTIFFPTTQVVSLTGITVEPTPATQFSLTD
                     VSVGAELGVSLPLLDLSLLAYHGWDTTPLYQPSFEFPNGFFGDYDIILTPVARKIDSI
                     GAEATLSWGSLSAWADGAYTFSKTFLSRKLSSSTYASDLVTSPCLEYAAGAGYDFSAP
                     RLSLQAGYRGLWVTQSTAETILPFLGSYLNGTCALRLWDDRLSVATQALVSTVDWSAA
                     IIGLLSIAPTNELSFDVVLPFFLGASDTELGQFAANYLVSIGVTVRY"
     gene            complement(4643..5377)
                     /locus_tag="VFH83_10350"
     CDS             complement(4643..5377)
                     /locus_tag="VFH83_10350"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HET6486811.1"
                     /translation="MRNRAWWILACLLMAAQASAQSADQLLNELSLFGSARFISMQVQ
                     MDLTLANGTKSRTLDVFIRRVLVRTSVLIHVVSPGFLSSMKFLSLHEGGQESSWIKTS
                     DGVRRLTDANGSERIFDSDFRAEDFMTVSVSRFSFAFLPDASIDGLPCIVIEARPRDA
                     AGYARKLFYIGKADLSLRGMDYLDSQGQLLRRYRMTASQSLAGSTAPLTAEMVDLRAG
                     TSTTLKVLKADVETPLPDSLFSRGTL"
     gene            complement(5374..>7156)
                     /locus_tag="VFH83_10355"
     CDS             complement(5374..>7156)
                     /locus_tag="VFH83_10355"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET6486812.1"
                     /translation="FRDFLLSRDRAATSIYVFLRRDAPVDRLVPAVQQACAAQTRARA
                     SVFGQELLRSSVRQSVVPELALLGGLSLLIIFLFEWVATRSLLAGLVLWVTNLLPALW
                     VLSFFPVAGLTLKTETIYAPIVLVALCTSYGLQLYRNLALGPGRGMGDALAEVTPVIF
                     SAGVTCILGFASLAASSIALLRLMGAILVMGIALGVLSSLFLLPTVLAMIPIRRYRRF
                     RRARALLGASRSRWVPVGFALAAAVLAFGTRWISVDYRVQSSIQGRSSLSRTAAQFDA
                     RYGGLDTLELIIDSRREYGWVDPVSYERLRSLRAELARVPQVTQVICVTDLVDWVRAR
                     LTGSANPPAGLDERQIGESLELLASAQAPVSLDGLLDPSYSITRLVVRFDTRGLGARD
                     SRVALRRLMGALESGARQRFPDAHLIVTGAAATTDRFLTLLVQSQVAGIFVYLLLAFL
                     FVAGFLRSARWALLSLLLPCGGVILYLGLLGWLRVPLTGVTVLTIASIMGVGIDGLLC
                     LITFYRTRRTSMASGPALEDALEASGMAIIQATLIIVLSLLCLLASRYSMFVWTGILT
                     GITFLGCMAVTLLLIPNLALRFERTLQ"
BASE COUNT         1354 a         2231 c         2477 g         1094 t
ORIGIN      
        1 gtaggtcacc ggctgggaga agtcattggt ggtggtcccg ctcgcctgga tggtggcgcc
       61 caccagaacc aacgcgcccg tgctgacgaa ctccgcgacg aggtgggtca cgtcggtgtc
      121 ggacgggacg gtcacggaga tcgtgttccc ggtctggtcg ataacgccga agaccgcggg
      181 tgagacgaag ccaaagcttg tcagccgttt gtcgctcgcg ctggcagcgc tcaccgtcac
      241 caggtaggcc tgggtgctcg cgtccgcggc aaccaccgtg tagctcacgg ggttggtgaa
      301 gtcattgggg gtgacgccgc tcgcctgaac ggtggcgccg atcttcacct gagcgccggt
      361 ggtggtgaac gtggctacca gactggtcac gttggtcccg aagggcaccg tcaccgcgat
      421 ggtatgcgcc gtctggttga tgactccggt ggcgccgatg gtcggaaagc tgaacgcggt
      481 caggctcttg ccgctggcgc ttgccgccgt aaagacggcg gtcaccagct tgtccgcatc
      541 catggtgagg gtcaccgggt tggcggtccc gctcgcgtcc cccgaccagt tggagaaggt
      601 gtagccgctg ttggcggtcg cggtgacctg gaccttgtct ccgttggagt agttggtgag
      661 atccggcacc cgggtgatgt accccgcctc actcggagaa accgtcacgg tcagggtgtg
      721 ggacaggccg agaatcgaca ggagctgctg gcacccactg agcccggtga tcacgagaat
      781 ggtgagtccg atagcaccga cgatggctgc gcgccccggt ttgttgattc gtgttgacat
      841 ttcggcacct tcccttttca ctgtggcagc accggggcga agtccacccc gaggacgtag
      901 ggaccattcg cgttctgcat catgtcggcg accgcccacc agaaggatcc cgtggtcgtg
      961 taactgatgt ccggcgccag gtctccgttc ccatccacgg caatggccgt gcccgccgca
     1021 acaaacgagt tgtcggcaac gaacgccggg aaggcgggca ggtacgccgt gatcgccccg
     1081 gtagtggggt tgcgcaccag gaggccgtcc agcgccgtct tgctgagcag gccggaggtg
     1141 gccgcggcta tcacgtcctt caggttgccg agcgcggtgt cgtcgatagc cagagtgcca
     1201 cccagggtca cgtccttgag tcggatggcg tagaagtggt acgcgcccag gttggccgcg
     1261 atagcgccga tgctggtccc ctgggacagg gcggccacga cctctggcgc gccgatgccg
     1321 gacagcgggg cgtagtcgct gtccgtgatt gcggaatcga tcaggaccgg ggaccctccc
     1381 tgctgcgtgc ccgccgagaa ggtcagggag aacggatacc agttgggctc accgttgctc
     1441 ctgagatcga gcagggagtc cagggccgcc agaggcgtca caaaggcggg ggcaaggttc
     1501 acgccgatgg actgaggggg gccggagtac ggcccgtcat ccggcgtcgc cggccagttg
     1561 gactccgttg cgtagtactg gatgaactcc atcggcccgg ggaagctgcc gaagttggtc
     1621 ggtatgacca tgacatggcc attctggacc gagtcgctca tcttcgtgat agcgatgtgg
     1681 gagtagttca ggacgtcgga cgcggtctgc cagttggcgg aaatgtccgg tatcgaggag
     1741 aactccgaaa tggtgaatgg cgagccctcc ccgcgatcca ggatcatcgt gaccgcctgg
     1801 tccaccgagc tcagtgccgc cacgtacgcc gtctttgccg ccgcaagggt cgttgcggca
     1861 tccgggcgtg cgcaaaggaa gccgccctgg atcgggttgg ggagggtgta cagcgccgcc
     1921 aggatcaggc tcgtcgtatt cagctcgggg ttgtcgaccg gattgagcgt gttgaagtac
     1981 tgctggaccg gaaaatcgag gcttacggtc ttggccatga gcagatcagc cttcatcatc
     2041 tgcaggaacc cggtcaggcc gagcagttcc gctttgccga tggaaatcgt ggccggctga
     2101 ctgctcacgt ccaacggcca gccgctactg aacgctccgc cgaacatgtc tgaggtgatg
     2161 tcgatctggg catcgtccgg gaggctggat gcctccaggg tattgaccac ctggtccagc
     2221 gccgtggaga ggatcccgtt caccacgtgg tccgcaaggc tgttgaaccc gttggggttg
     2281 tgcgttgcca ggttgtagag gacagccatc gagtattcgc cgggggtcac gaccgccggg
     2341 ttgccggccc acgcctggat ggtggcgggg atgcggacct gggggacgta cgagaggtac
     2401 gactcaaacg cgtagtctcc ggtccccggt tctacggaca tatacgaatt ggggcccgtc
     2461 gagacccaat cgccgttgtt cgggccgacg tagacaaagt gcatggcgcc agcgtcaacg
     2521 gcctgaccca tccacgcggg gaccccgtgg ggacccggct ggaagaaggc gtccatgctc
     2581 gtcgggtagt tctcgaaccc cagataggtg gtggccagcg tctgcacgct ggaagtcgtg
     2641 atggtctggg caagcgtgag gaggcacgtc cacaggagcg cggtgtagtt ggtgggatcg
     2701 gactgcaggg acctggagaa cgcggtcagc gcgcgcgtgt agtccggcgg cctctggctc
     2761 aggtaggcct tcccggccgc gaccagcttc tccgcttccg ccccgtccgc cgtccgcgcg
     2821 gataccgcgg cgctcggcgc gccctccccg cccgcggccc ggtacgcgcg caccttgtag
     2881 gagtagctga ctcctgacgt cacgcccgag tccttccagg caagggccgt tacgccctgc
     2941 gccaccaggg agtacgggcc accgtctgac gaccggtaca cccgatagct ctcggcgccc
     3001 gatacggggg accagctcag gccgatgtag acggtgctgg gggtgacctg cagggtccca
     3061 ggagccgcga gggaggccga cgctgagggg ttggagagcg ggaagctaca ggccattgcc
     3121 agcagcacca ggacgaggac gggaggcacc agagggacac gtttcgccca actcgaatgc
     3181 cgcatacttc ctcctgtact ttcaaatcct gccgggacgc acggctgttc cacgtcgccg
     3241 ccacggccag tattctcatt cgctctggcg tcaggatatc actacactgc cctccgcgca
     3301 agaggactcg gaccggcggc gaagcgtcag taccggacgg taacgccgat agataccagg
     3361 tagttggccg cgaactgacc gagctccgta tcggatgccc cgaggaagaa cggaaggaca
     3421 acgtcgaatg aaagctcgtt ggtcggcgcg atggacaaga ggccgatgat cgcggcgctc
     3481 cagtccaccg tggataccag cgcctgggtt gccacggaaa gtcgatcatc ccagagccga
     3541 agcgcgcacg tcccgttgag atacgacccc aggaagggca ggatcgtctc cgcggtgctc
     3601 tgcgtcaccc aaagcccgcg atagcctgcc tgcagcgaga gcctgggcgc cgagaagtcg
     3661 tagccggcgc cggccgcata ctccaggcac ggcgaggtga ccaggtccga agcgtaggtg
     3721 ctgctggaga gcttgcggga caggaaggtc ttggagaagg tgtaggcgcc atccgcccag
     3781 gcgctgaggg aaccccagct cagcgtcgcc tccgcgccga tggaatcgat cttgcgggcc
     3841 accggggtca ggatgatgtc gtaatccccg aagaacccat tggggaactc gaagctgggc
     3901 tgatatagcg gcgtggtgtc ccagccgtgg taggcaagca ggctcaggtc cagcagcggg
     3961 agggagacgc ccagctccgc ccctacgctg acgtcggtaa gactgaactg cgtggcgggg
     4021 gtcggctcca cggttatccc cgtcagggaa accacctgcg tcgtcgggaa aaagatggtc
     4081 aggttccggg ggaaatcctt tttcggaaac cagggcgagc tgggatccgg gatactccag
     4141 gcgggagcaa acggagaggc gctcagggtg agggaccaat cccccagggt caagatgccc
     4201 tgaaccaggt ccccacctcg gaccagggac tccgtgtcgt tacggaccag ggccaggaga
     4261 tccggcgtca cgaaccagtg ggtaggagag agaacgcccg ccgaaccggg cgcatagacg
     4321 aacctgccga gcctcaaggt gaggaatccc gccggtgata gctcgatgta aaggtcccgg
     4381 atgcccaggc tcggggccac ggccgcgcgg tcccactcca gggaaagctt cccgtccagc
     4441 cgccaggcag ggtcctccac gtggatcgta gggtagaaca cgatggcgtc gtcgatgctg
     4501 gatgcaagag gctgcgacgg gtcgaccaac gccgagacgc ccaggttcag ctcgcctccc
     4561 gccaccgcct gcgcgccggc ctgcagggga accagcccca gcgcgattcc cagcgcgacc
     4621 cccagcgacg cccggcggcc ggtcacaggg tccctcgact gaacagggaa tccggcaggg
     4681 gcgtctccac gtccgccttg agcaccttca gggtggtcga cgtgcccgcg cgcaggtcca
     4741 ccatctccgc ggtgaggggc gccgtgcttc ccgccaggct ctgggaggcg gtcatgcggt
     4801 agcggcgcag gagctgaccc tgggaatcca ggtaatccat tccgcgcagg ctgagatccg
     4861 ctttcccgat gtagaacagc ttccgggcgt agccggcggc atcccgcggc cgggcctcga
     4921 tcacgatgca cggcagccca tcgatggacg catccggcag gaatgcgaaa gagaaccggc
     4981 tcacggagac ggtcatgaag tcctccgcgc ggaagtcgga atcgaagatc cgctcggaac
     5041 cgttggcgtc cgtgagccga cggacaccat cggaggtctt gatccaggag ctctcctggc
     5101 cgccctcatg caggctcagg aacttcatgg agctgaggaa tccgggcgac accacgtgga
     5161 tcaggacact ggtcctgacc agcacgcggc ggatgaagac gtcgagcgtc cggctcttgg
     5221 tcccgttggc cagggtgagg tccatctgga cctgcatcga gatgaaccgg gcggacccga
     5281 acaggctcag ctcgttcaga agctggtccg cgctttgcgc ggatgcctgg gcggccatga
     5341 gcaggcacgc cagtatccac caggctctgt ttctcattgc agcgtccttt cgaacctgag
     5401 cgcgaggttg ggaatcagaa gcagcgtgac cgccatgcac cccaggaagg tgatgccggt
     5461 caggatcccg gtccagacga acatagagta gcgtgacgcg agcagacaga gcaaggacag
     5521 cacgatgatg agcgtggcct ggatgatcgc catcccggag gcctccagcg catcctccag
     5581 cgcgggcccc gaggccatgg acgtccgacg ggtccgatag aaagtgatca ggcagagaag
     5641 cccgtcgatg ccgacgccca tgatcgaggc aatcgtcagg accgtgacgc cggtcagcgg
     5701 caccctcagc caccccagca ggcccagata gaggatcaca ccgccgcacg gaagcaggag
     5761 ggagagcagc gcccaccgcg cggagcgaag aaagccggcc acaaagagaa atgcaaggag
     5821 aaggtagacg aagatgccgg caacctggct ctggaccagc agggtgagga atcggtccgt
     5881 ggtggcggcc gctcccgtca cgatgaggtg ggcgtcgggg aaccgctgcc gggcgccgga
     5941 ctccagcgcc cccatgagcc ggcgcagcgc gacgcgggag tctcgagccc ccagtccccg
     6001 ggtatcgaac cggacgacca ggcgcgtgat ggaataggag gggtcgagga gcccgtccag
     6061 agataccggg gcctgcgcgg aggccagcag ctccagcgat tcgccgatct ggcgctcatc
     6121 cagcccggcg ggcgggttgg cgcttcccgt cagccgggcg cggacccagt ctaccaggtc
     6181 cgtcacgcag atcacctggg tcacctgagg gacacgggcc agctccgcgc gcagggacct
     6241 caaccgctcg taggagaccg gatcgaccca gccgtactct cgccggctgt cgattatcag
     6301 ctccagggtg tccaggccgc cataacgagc gtcgaactgg gcggccgtgc gggacaggga
     6361 ggacctcccc tggatcgagc tctggacgcg gtagtccacg ctgatccagc gcgtgccgaa
     6421 cgcaagcacg gccgccgcca gagcgaagcc cacgggaacc caccgggacc gggacgcgcc
     6481 cagcagcgcc cgggcccggc ggaacctccg atagcggcgg atcgggatca tcgccagaac
     6541 cgtcggcagc aggaacaggg aggagaggac tcccagcgca atccccatca ccaggatcgc
     6601 ccccatcagg cgcagcagag cgatggagga ggcggccagg gaggcgaaac ccaggatgca
     6661 ggtgacgccc gcggagaaga tgaccggagt cacctccgca agcgcgtcgc ccatgccgcg
     6721 tcccggaccc aaggccaggt tgcggtagag ctgcagacca taagaggtgc agagcgcaac
     6781 gagaacgatg ggggcgtaga tggtctccgt cttgagggtg agtccggcaa ccgggaagaa
     6841 cgagaggacc cagagcgccg gcaggaggtt cgtcacccac agtaccaggc cggccagcag
     6901 cgatcgggtg gcaacccatt cgaacaggaa gatgatcagg agcgaaagac cgcccagaag
     6961 ggccagctcg gggaccaccg actgccggac ggagctccgc agcagctcct gaccgaatac
     7021 gcttgcccgg gcgcgcgtct gcgcggcgca cgcctgctgg accgcgggca cgagccggtc
     7081 cacgggcgca tccctccgca ggaatacgta gatcgaggtc gcggcccggt ctcgggaaag
     7141 gaggaagtcc cgaaag
//
LOCUS       DASRZS010000424         5998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_618051, whole genome shotgun sequence.
ACCESSION   DASRZS010000424 DASRZS010000000
VERSION     DASRZS010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618051"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFH83_10360"
     CDS             complement(<1..626)
                     /locus_tag="VFH83_10360"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HET6486813.1"
                     /translation="MNPDQHRKTQFSIFYVVVGLLVILGLQWILGGGLSTDLPYSDFK
                     KALSDGKVQEVLISPTDIQGVLSGQNGQTTTFHSIRVDDPSLVQQLQEHSVKFSGKAQ
                     GSFLGNILSWIIPLGLMFALWNFFMLRMGSGGPGVLSIGQSKAKLVADTGAKRITFGD
                     VAGIDEAKEELQEVVEFLKTPQRFETLGGRIPRGILLVGAPGTGKTLLA"
     gene            857..1855
                     /gene="trpS"
                     /locus_tag="VFH83_10365"
     CDS             857..1855
                     /gene="trpS"
                     /locus_tag="VFH83_10365"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET6486814.1"
                     /translation="MKVLFSGIQPTGDIHLGNYFGAIANWVRLQYEYRSYISVVDLHA
                     VTIPYEVADMPARVRQLALTLYACGIDLEKTTLFVQSEVPGHADLTWLFNTVTPLGEL
                     ERMTQFKDKREQFRASVNVGLLDYPVLQAADILLYKAEVVPVGEDQVQHVEFTREVAR
                     HFNARFGQTFPECQALLTTAPRVPGLDGDSKMSKSKGNTIGILEEPEQIWKKLAPAKT
                     DPARVKRTDPGTPEKCTIWSYHLLVTPETERPDIHDGCTTARIGCIDCKKRLHANLMK
                     VLDPIRQRYAELDGARRPEVEARMEENASRCRAVAAQTMLEVKEKMGFRKVWKIES"
     gene            1837..3174
                     /locus_tag="VFH83_10370"
     CDS             1837..3174
                     /locus_tag="VFH83_10370"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET6486815.1"
                     /translation="MEDRELTAEDFAVETIGEPRVPCPLGLGTTRGENKANYVADSHT
                     VLMEHDRELVAQALRQGKYVPAFQAAGPRERIFYDPSSTRVAIVTCGGLCPGINNVIR
                     TLVNELHYLYGVRSILGIRYGYRGFIPRYGYEPYILTPDFVKDIHEKGGSILSSSRGE
                     QDSAEVVEFLQRRGVSILFAIGGDGTLRGALDIQKEVKRRKLPIAVIGIPKTIDNDIS
                     YVERTFGLETAFAVAAESIRSAHTEAIGAPNGVGIVKVMGRMSGYIAANAALALAEVN
                     FVLIPEVPFGLDGTYGLYALVEQRLRQRDHAVIIVAEGAGQDYVKDPQGRKDDSGNDV
                     LGDIGVFLKDSIKKYFKERSNIFVNLKYIDPSYLVRSVPANAHDSIYCIQLAQNAVHA
                     GMAGKTGMLIGQWNGQFTNVPIALATSSRKVLSLEDPLWLSVLQATGQPERIG"
     gene            3367..4527
                     /gene="metK"
                     /locus_tag="VFH83_10375"
     CDS             3367..4527
                     /gene="metK"
                     /locus_tag="VFH83_10375"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET6486816.1"
                     /translation="MAQRKSFTFTSESVGEGHPDKICDQIADAVLDDAIRQDATSHVA
                     CESFTTTGLVLVGGEITTQGYIDLQQIVRGVLKSIGYDDPSYGIDYQSCSVVSTIQRQ
                     SPDIAQGVNGTGLHHEQGAGDQGMMFGYACNETPTLMPAPISLAHRIMQIAAKSRKDG
                     SLPFLRPDGKCQVSVEYEDDKPVRVSTVVLSHQHSEEVLYKELQEALVEKVIKVAIPR
                     DLLTPKTVYHINPTGRFVIGGPHGDTGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVD
                     RSASYMARYVAKNIVASGVCDRCEVQVAYAIGIAKPVSFYVSTFGTGKVPDERIERAV
                     SEVFELTPHGIIETLDLLRPIYQQTTNYGHFGREGFSWERTDKAQAIKSKLG"
     gene            4466..5554
                     /gene="amrS"
                     /locus_tag="VFH83_10380"
     CDS             4466..5554
                     /gene="amrS"
                     /locus_tag="VFH83_10380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HET6486817.1"
                     /translation="MRGSPGSAPTRHRPSSPNWGSTTEPRPASLPRARWFEGRPDGRV
                     QCLLCPHACRIREGGTGTCGVRRAERGAVTLPYYGRISALAVDPIEKKPLYHFHPGER
                     ILSAGFVGCSLRCKFCQNWHISQSTDSPTRFVAPDELVDMAREERSFGIAYTYSEPLI
                     HAEYVIDAARAARAAGLANVLVSNGYLNPEPAAAVLELMDAANIDLKSYDADFYRSET
                     GGDLDSVRRFIAEAGRRTHLEVTTLVIPTRTDDDEQIDGIARFVASIDPGIPLHLSCY
                     YPQYRYKIPPTPAATVERLAAVARKHLRFVYLGNIGPSETNTLCPGCGALLVRRLGYA
                     VRVEGLERRGASSACARCGTAVPIALGA"
     gene            5768..5840
                     /locus_tag="VFH83_10385"
     tRNA            5768..5840
                     /locus_tag="VFH83_10385"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5801..5803,aa:Asn,seq:gtt)
     gene            complement(5809..>5998)
                     /locus_tag="VFH83_10390"
     CDS             complement(5809..>5998)
                     /locus_tag="VFH83_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486818.1"
                     /translation="QGRIQEALKRPEPEMKPALAELVSSLLLEDGQIKIALSSRNQRA
                     LHSSLFAPSPPAGLEPAT"
BASE COUNT         1095 a         1959 c         1906 g         1038 t
ORIGIN      
        1 gcgagcagcg tcttgcccgt gcccggggcg cccaccagga ggatgccccg tgggatgcgg
       61 cccccgaggg tctcgaatcg ctgcggggtc ttgaggaact ccactacttc ctgcagctcc
      121 tccttggcct cgtcgatccc cgccacgtct ccgaacgtga tgcgcttggc gccagtgtcc
      181 gccacgagct tggccttgct ctgcccaatg ctgagcacgc cgggccctcc gcttcccatg
      241 cggagcatga agaagttcca gagcgcaaac atgagaccca acgggatgat ccaggacagg
      301 atgttgccca ggaagcttcc ctgggccttg ccggagaact tcaccgagtg ctcctggagc
      361 tgctgcacca ggctcgggtc gtccacccga atgctgtgga aggtcgtggt ctgcccgttc
      421 tggcccgaaa ggacgccctg gatgtccgtc ggggagatca gcacctcctg caccttgcca
      481 tccgacagcg ccttcttgaa atcgctgtaa ggcaggtccg tggagagccc tccccccagg
      541 atccactgga ggcccaggat gaccaggagc cccaccacga cgtagaagat cgagaactgc
      601 gtttttctat gctggtccgg gttcatggta tgcacccctc ccctttccgt gcggacacat
      661 cgctgcgtgt ccgttccccc ttcgcggggt ttcctcgacg gtcagaagca tcccgctcct
      721 cttctcgcgc accttccgag ggatcgcccc tctggcgacg gccccctctc gtgccgtaat
      781 gaccatctct ggaatcaatc taagcctgta caacgtcaaa aacaagcgct ataaaggccc
      841 gcagggagga gcgcgcatga aggtactgtt ctctggaatt caacccacgg gcgacatcca
      901 cctcggcaac tacttcgggg ccatcgccaa ctgggtgcgg ctccagtacg agtaccgctc
      961 ctatatcagc gtggtggacc tgcacgcggt caccatcccg tacgaggtgg cggacatgcc
     1021 cgcccgcgtg cgtcagcttg ccctcactct ctacgcgtgc gggatcgacc tggagaagac
     1081 gacgctcttc gtgcagtccg aggtcccggg gcatgccgac ctcacgtggc tcttcaatac
     1141 cgtgacgccg ctgggagagc tggagcgcat gacccagttc aaggacaagc gcgagcagtt
     1201 ccgggcttcg gtgaacgtgg gtctgctgga ctaccccgtg ctccaggccg cggacatcct
     1261 cctctacaag gcggaggtgg ttcccgtcgg ggaagaccag gtccagcacg tcgagttcac
     1321 ccgggaggtg gcccggcact tcaacgcccg gttcggccag acgtttccgg agtgccaggc
     1381 gctcctgacc acggcgcccc gcgtgcccgg gctggacggc gacagcaaga tgagcaagag
     1441 caagggcaac accatcggga tcctggagga gcccgagcag atctggaaga agctggctcc
     1501 cgccaagacg gatcccgcgc gggtcaagcg gacggatccc gggacgccgg agaagtgcac
     1561 catctggtcg taccaccttc tggtcacgcc ggagaccgag cgccccgaca tccacgacgg
     1621 ctgcacgacg gcgcgcatcg gatgcatcga ctgcaagaag cggctccacg cgaacctgat
     1681 gaaggtcctg gaccccatcc gccagcgcta cgcggagctg gatggggccc gcaggccgga
     1741 ggtggaggcg cgcatggaag agaacgcgtc ccgctgccgc gcggtggccg cccagaccat
     1801 gctcgaggtc aaagaaaaga tggggttcag gaaagtatgg aagatcgaga gctgaccgcg
     1861 gaggacttcg cggtagagac catcggggag cccagggtgc cgtgtccgct ggggctgggc
     1921 acgacgcgtg gggaaaacaa ggccaactac gtggcggatt cccacaccgt gctcatggag
     1981 cacgatcggg agctggtggc ccaagcgctc aggcagggga agtacgtacc ggccttccag
     2041 gccgcggggc cgcgggagcg catcttctac gatccctcct cgacgcgggt cgccatcgtc
     2101 acctgcggcg ggttgtgccc gggcatcaac aacgtgatcc gcaccctggt caacgagctg
     2161 cactacctct atggggtgcg ctccatcctg gggatccgct acgggtaccg cggcttcatt
     2221 ccccggtatg gctacgagcc ctatatcctg acgccggact tcgtcaagga catccacgag
     2281 aaaggcggga gcatcctctc ctcctcgcgc ggggagcagg attccgcgga ggtcgtggag
     2341 ttcctccagc gcaggggcgt gtccattctc tttgcgatcg gcggtgacgg gaccctccgg
     2401 ggcgccctgg acatccagaa ggaagtgaag cggcggaagc tgcccatcgc cgtgatcggg
     2461 atccccaaga ccatcgacaa cgacatctct tacgtcgagc ggaccttcgg gctggagacc
     2521 gcctttgccg ttgcggcgga atccatccgc agcgcgcaca cggaggcaat cggcgccccc
     2581 aacggcgtgg gcatcgtgaa ggtgatgggc cgaatgtccg gctacatcgc cgccaacgcg
     2641 gcgctggccc tggcggaagt gaacttcgtc ctcatcccgg aggtgccctt cgggctcgat
     2701 ggcacgtacg gcctttacgc gctggtggag cagcgcctgc ggcagaggga ccacgcggtg
     2761 atcatcgtcg cggaaggggc gggacaggac tacgtcaagg atccgcaggg ccgcaaggac
     2821 gactccggca acgacgtgct gggggacatc ggcgtgttcc tcaaggactc catcaagaag
     2881 tacttcaagg agcgctccaa catcttcgtg aacctcaagt acatcgatcc cagctacctg
     2941 gtccggtcgg ttcccgcgaa tgcccatgac tccatctatt gcatccagct tgcgcagaac
     3001 gcggtccacg ccggaatggc gggcaagacg gggatgctca tcgggcagtg gaacgggcag
     3061 ttcaccaacg tacccatcgc gttggccacc tcaagccgca aggtgctctc cctcgaggac
     3121 ccgctctggc tctcggtgct ccaggcaacg gggcagcccg agcgcatcgg gtgacgggcg
     3181 caccacattc gcatcgagtg agcttgacga ggggcggcgg gcgcgatact ctctctcatg
     3241 gaccgttggt cccgtggagt gatttgcggt gtattgcaac cacgtaaaaa agtgctaaaa
     3301 aaccaggacc ttcccagcaa gcccgcctgt gttttttaga caccgaccac ccttcggagg
     3361 cgtgtcgtgg cccagcgaaa gagtttcacc tttacctcgg agagcgtcgg ggagggacac
     3421 cccgacaaga tctgcgacca gatcgcggac gccgtgctgg atgatgccat ccggcaggac
     3481 gcaacctcgc acgttgcctg tgagagcttc accaccaccg gtctggtgct ggtaggcggg
     3541 gagatcacca cccaggggta catcgacctg cagcagatcg tgcgcggcgt tctgaagtcc
     3601 atcgggtacg atgacccgtc gtacgggatc gactaccagt cctgttccgt ggtctccacc
     3661 atccagcggc agtccccgga catcgcccag ggcgtcaacg gcacgggcct ccaccatgag
     3721 cagggcgcgg gggaccaggg gatgatgttc ggctacgcct gcaacgagac gcccaccctc
     3781 atgcccgccc ccatcagcct ggcgcaccgc atcatgcaga tcgcggcgaa gtcccgcaag
     3841 gatgggtccc tccccttcct gcggcccgac ggcaagtgcc aggtctcggt ggagtacgag
     3901 gatgacaagc ccgtccgggt gtcaacggtc gtgctctccc accagcactc cgaggaggtc
     3961 ctctacaagg agctccagga agccctggtg gagaaggtca tcaaggtggc cattcctcgc
     4021 gacctcctga cgcccaagac cgtctatcac atcaacccca cgggtcggtt cgtgatcggc
     4081 ggtccgcatg gagacacggg gctcaccggc cgcaagatca ttgtggacac gtacggcggg
     4141 atggcccgcc acggcggcgg cgcgttctcc ggcaaggacc ccagcaaggt ggaccggtcc
     4201 gcgtcgtaca tggcgcgcta cgtggccaag aacatcgtcg cctccggcgt ctgtgaccgc
     4261 tgcgaggtgc aggtggcgta cgccatcggc atcgccaagc cggtctcctt ctatgtatcc
     4321 accttcggaa cggggaaggt tcccgacgag cgcatcgaga gggccgtgag cgaggtcttc
     4381 gagctcacgc cgcacggcat catcgagacc ctcgacctgc tgcggccgat ctaccagcag
     4441 accaccaact acggtcactt tggtcgtgag gggttctcct gggagcgcac cgacaaggca
     4501 caggccatca agtccaaact ggggtagcac cacggaaccg cggccggcct ccttaccacg
     4561 cgcccgctgg ttcgaaggcc ggcccgacgg tcgggtgcag tgcctgctct gccctcacgc
     4621 gtgtcggatc cgtgagggcg ggacgggcac ctgcggcgta cggcgcgcgg agcgcggggc
     4681 ggtgaccctg ccgtactacg gcaggatctc ggccctcgcg gtcgatccca tcgagaagaa
     4741 gcccctctat cacttccacc ccggggagcg gatcctgtcg gccggcttcg tgggatgctc
     4801 cctgcggtgc aagttctgcc agaactggca tatctctcag agcaccgact caccgacacg
     4861 gttcgtcgct cctgacgagc tcgtggacat ggcccgagag gaaaggtcct tcgggatcgc
     4921 ctacacgtat tccgaacccc tgatccacgc ggagtacgtg atcgatgccg cgcgggccgc
     4981 gcgcgcggcg gggctggcca acgtcctggt ctccaacgga tacctgaacc cggagccggc
     5041 cgccgcggtg ctggagctta tggatgccgc caacatcgac ctcaagtcct atgatgccga
     5101 cttctaccga tcggagacgg gtggcgatct ggactccgtg aggcggttca tcgccgaggc
     5161 cgggcggcgc actcacctgg aggtcaccac actggtgatc ccgacccgaa ccgacgatga
     5221 tgagcagatc gacgggatag ctcggttcgt ggcatccatc gatcctggga tcccgctcca
     5281 cctctcctgc tactatccgc agtaccggta caaaatcccg ccaacgccgg cggccaccgt
     5341 ggagcggttg gcagcggtcg cacggaagca cctgcggttc gtctacctgg ggaatatcgg
     5401 cccctctgag accaacacgc tctgccctgg ttgcggagcc ctactggtgc gccggttggg
     5461 gtatgcggtc cgggtggagg gccttgagcg gcgcggagcg tccagtgctt gcgccagatg
     5521 cggaacggcc gtgcccatcg cgcttggggc ttgaggcggc tcgctttgcc gccggcgctt
     5581 tctggaaggc atggctccgc attgtcgaaa tctgcagaat cgcaccgtgc gttttcgcag
     5641 attcccacag gggacgcacc aggtgccggg aaaacctggc tggcgatcga tgactcagag
     5701 gatctcgacc ctctcgaccc gacttgacaa cgcacctgtt ttctggcttt tatacgtttg
     5761 acgacattcc cctgtagctc agtcggtaga gcgggtggct gttaaccact aggtcgctgg
     5821 ttcgagtccg gccgggggag atggggcgaa caagctcgaa tgaagcgctc tctggttccg
     5881 gctggagagc gctatcttta tctgcccatc ctccaacaag agagacgaga ccagctccgc
     5941 gagggccggc ttcatctcgg gttcgggtct cttgagggct tcctgaatcc gtccttgg
//
LOCUS       DASRZS010000425         4502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5619021, whole genome shotgun sequence.
ACCESSION   DASRZS010000425 DASRZS010000000
VERSION     DASRZS010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5619021"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VFH83_10395"
     CDS             <1..494
                     /locus_tag="VFH83_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HET6486819.1"
                     /translation="FFYTGEPLNYKRRGLAVALVFFLMGVFMVAGSYYAVTGWLAPRV
                     IVSSVPVSLLVSLILLANEMRDYESDVRYGIGTLTVRIGYRAAVILYCCLLASAYGGA
                     LLLAPLGVIAHGWLVLLALPFAIPPFVFMPRPPRQRMPIIPFVMLHHLAFGALFCASY
                     FIS"
     gene            506..1450
                     /locus_tag="VFH83_10400"
     CDS             506..1450
                     /locus_tag="VFH83_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6486820.1"
                     /translation="MHARRPFLVLIAALGFAALVLVPRGFADLTVGLMPAYNSVPLVV
                     AQAKGFFAQERVQVTLVPFSSQLNRETALQTGAIDGTVSDMINAIQAWAQGFGGKVTS
                     VTEGGFSLLASKQSGIRTLADWDRKQKVSTGLLQNSIVFYTAERMLSETGHDPAKIDL
                     VPIVQVPARLEMLAAGKIDAACLPEPLATLAVSQGASRLADTDSIGSTTGVLLFGAKA
                     LAAKEPEVRAFYRAYDRAVAVVNADWTPYRDQIVQACEFPPPVAPILKVPRFRASFLP
                     SPDQVQDVARWMVAKGLVTRAPAYSDVVAEGFVGASAP"
     gene            1410..2174
                     /locus_tag="VFH83_10405"
     CDS             1410..2174
                     /locus_tag="VFH83_10405"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486821.1"
                     /translation="MSSQRASWVLQLHECSAGYAGQPVVQGISLSVGEGKTACVIGPS
                     GCGKTTLLKVAAGLLAPSAGSVLLDGTPVALADRRVSIIFQSYGLFPWLTALQNVALG
                     LRLRGVPRRERDRIALGELDRLGLGDLSRRYPARLSGGQLQRVAIARSMALAPRLLLL
                     DEPFSALDALSREDLQEILLETLAAHRLCALMVTHSIEEAVFLGSTIWILGGSPARIV
                     SCLENAGQGAPGFRASPAFFEHVTAIRRTLERSRDH"
     gene            2171..2956
                     /locus_tag="VFH83_10410"
     CDS             2171..2956
                     /locus_tag="VFH83_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6486822.1"
                     /translation="MSGTRTPASAPSHGPASLGFVVVAVAAIVGAWWCLSLAIGGRLV
                     PDPPSVFRRFVVLLPGILGASVLVSLARVGAALAAALLTAVPLGVLLGRNRLLDRVLS
                     PIAYLSYPVPKIALLPVIMLLFGLGEWAKGIVIFLVLFFQTLVTVRDAARAVPEPYVL
                     SIRSLGATRPQVWRYVLWPALLPSLLSSLRIGTGTALAVLFFAETFGTTVGLGWFVME
                     SWMRMSYVDLYAGILCLALLGLALFLAIDLIQRWSCRWQAAPP"
     gene            3015..3899
                     /gene="rfbA"
                     /locus_tag="VFH83_10415"
     CDS             3015..3899
                     /gene="rfbA"
                     /locus_tag="VFH83_10415"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET6486823.1"
                     /translation="MKGIILAGGSGTRLYPVTQVVCKQLLPVYDKPMVHYPLSILMLS
                     GIRDILIISTPADLPRFRELLGSGAQLGLRFSYAVQDAPRGLADAFRVGADFIGHDSV
                     SLVLGDNIFYGHGLPEVLRNASRQEGGATVFGYYVNDPERYGVIEFDQARRVVSIEEK
                     PARPKSKYAVVGLYFYDNQVVQIARDLKPSPRGELEITDVNRRYLEMGQLRCELMGRG
                     FAWLDTGTFSSLVDATLYIKAIEDRQGLKVACVEEIAYQMGYIGAAELRSLAEPLRKS
                     GYGEYLLRLLEQGPTLGG"
     gene            3904..4467
                     /gene="rfbC"
                     /locus_tag="VFH83_10420"
     CDS             3904..4467
                     /gene="rfbC"
                     /locus_tag="VFH83_10420"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET6486824.1"
                     /translation="MPFTFSPAALRGLVIVQPRIFPDQRGFFLESYKASDFERAGLPE
                     RFAQDNHSLSERGVLRGLHYQLPPFAQGKLVRVLEGRVWDVAVDIRPDSPTYAKWFGL
                     ELSAEDHRMLYVPPGFAHGFLTLSPRAQFFYKCTAEYRRESEAGIRWNDPAIGIAWPV
                     TEVTVSDRDEGLPLLKDATVFPAGLYP"
BASE COUNT          639 a         1479 c         1480 g          904 t
ORIGIN      
        1 ggttcttcta taccggagag ccgctgaact acaagcgccg tgggctggcg gttgccctgg
       61 tgttcttcct catgggcgtc ttcatggtgg cgggatccta ctacgcggtc accggctggc
      121 tcgcgccccg ggtgatcgtt agctcggttc ccgtaagcct cctggtttcc ctgatccttc
      181 tggccaacga gatgcgggac tacgagtccg atgtgcgcta cgggatcggg acgctcacgg
      241 tccgcatcgg gtaccgcgcg gcggtgatcc tctactgctg cctcctggcg tctgcctacg
      301 gaggcgcgtt gctcctggct cccctcggtg tgattgccca cggttggctg gtactgctgg
      361 ccctgccgtt cgcgatcccg ccgtttgtct tcatgccccg gccgcccagg cagcgtatgc
      421 cgatcatccc tttcgtgatg ctccatcacc tggccttcgg cgcgttgttt tgcgcgagct
      481 acttcatctc ctgaaagagg aacccatgca tgcccgccgt cccttcctgg tcctgatcgc
      541 cgccctgggc ttcgctgccc tggtcctggt cccgcgaggg ttcgccgacc tgaccgtggg
      601 gctcatgccg gcctacaatt cggttcccct cgtggtggca caggcaaagg gcttcttcgc
      661 ccaggagcgg gtgcaggtca ccctggtacc cttctccagc cagctcaacc gcgagacagc
      721 gctccagacg ggggcgatcg acggcacggt ctccgacatg atcaacgcca tccaggcatg
      781 ggcacagggg ttcggcggca aggtgacctc cgtcacggag ggcggcttca gcctgcttgc
      841 cagcaagcag tccggcattc ggacgctggc tgactgggat cggaagcaga aggtctccac
      901 gggcctgctc cagaacagca tcgtgttcta cacggcggag cggatgctct cggaaaccgg
      961 gcacgatccc gccaagatcg acctggtccc tatcgtgcag gtgccggcgc ggctcgagat
     1021 gcttgctgcg gggaagatcg atgcggcatg cctgcccgag cccctggcaa ccctggcggt
     1081 ctcccagggg gcgtcccggt tggcggacac cgacagcatc ggttccacga ccggtgtgct
     1141 gctcttcgga gccaaagcgc tcgcagccaa ggagccggag gtccgcgcct tctaccgggc
     1201 gtatgaccgg gccgtggcgg tggtgaacgc cgattggact ccctaccgcg accagatcgt
     1261 ccaggcatgc gagttcccgc cgccggtcgc cccgattctc aaagtcccga ggttccgtgc
     1321 ctcgttcctt ccatcgcccg accaggttca ggatgtcgcg cgctggatgg tggcaaaggg
     1381 cctcgtgacc cgcgcgccag catactcgga tgtcgtcgca gagggcttcg tgggtgcttc
     1441 agctccatga gtgctcggcc ggctacgccg gccaaccggt ggtgcagggg atctccctct
     1501 ccgtggggga ggggaagacc gcctgcgtca tcggtcccag cggatgcggc aaaacgactc
     1561 tcctgaaagt cgccgccggt cttctcgcgc cgtcggcagg gagtgtactt ctggacggca
     1621 cgcctgtagc gctggcggac cggcgggtca gcatcatctt ccagagctac gggctctttc
     1681 cgtggctcac tgcgctccag aacgttgccc tgggattgcg gcttcgaggg gtgccccgtc
     1741 gcgaacgaga ccggatcgcc ctgggcgagc tcgatcgcct gggcctgggg gacctctccc
     1801 ggcgttatcc ggcccgcttg agcggcgggc agctccagcg ggtggcaatc gcccgaagca
     1861 tggcgctggc tccacgcctg ctcctcctgg atgagccgtt ctcggcactg gacgctctga
     1921 gccgggaaga tctccaggag atccttctcg agaccctcgc cgcccaccgc ctctgcgccc
     1981 tcatggtcac tcacagcatc gaagaggcgg tcttcctcgg ctccacgatc tggatcctgg
     2041 gaggctcccc cgctcggatc gtctcctgtc tggaaaacgc gggccagggc gcgcctgggt
     2101 tcagggcgtc ccccgccttc ttcgaacacg tcacggcgat ccgaaggacc ctcgagagga
     2161 gccgagacca ttgagcggga cccgcacgcc agcgtccgcg ccgtcccacg gtccagcatc
     2221 tctggggttc gtggtcgtcg cggttgccgc aatcgtcggc gcgtggtggt gcctgagcct
     2281 ggcgatcggc ggccgactgg tgccggaccc gccgtccgtc ttccggcggt ttgtcgtctt
     2341 gttgcccggc atcctggggg ccagcgtcct ggtgagcctt gcgcgcgtgg gggcagcgct
     2401 ggcggccgcg ctcctgacgg ccgtgccctt gggcgttctc ctgggccgca accgcctcct
     2461 ggaccgtgtg ctctcgccga tcgcctacct ctcgtatcct gtgcccaaga ttgcgctcct
     2521 gcccgtgatc atgctgctct tcgggctggg cgagtgggcc aagggaatcg tcatcttcct
     2581 cgtcctcttc ttccagaccc tggtcaccgt ccgggacgcc gcacgggcgg tgccggagcc
     2641 ctacgtgctc tccatccgct ccctgggcgc cacgcggccc caggtctggc gctacgttct
     2701 ctggccggcg ctgcttcctt ccctgctttc gtcgttgcgg atcgggacgg gaacggcgct
     2761 ggccgtgctc ttcttcgcgg agaccttcgg gacgaccgtg gggcttggct ggttcgtcat
     2821 ggagagctgg atgcggatgt cctacgtcga cctgtacgct ggcatcctct gtctggcgct
     2881 cctcgggctg gcgctcttcc tggcgatcga cttgatccag cgctggagct gccgctggca
     2941 agccgcgcca ccttgacgta cggccggaga cgcaagtata aggacgtaaa cgtggggggc
     3001 tgcgcatacc cgatatgaaa ggcatcattc tcgccggagg ctcaggcacc aggctctacc
     3061 cggttacgca ggttgtgtgc aagcagcttt tgcctgtcta cgacaagccg atggtgcact
     3121 atccgttgtc catcctgatg ctctcgggca tccgggacat ccttatcatc tccacgccag
     3181 cggatctccc tcggttccgt gagctcctgg gctccggtgc acagcttggc ctgcgattct
     3241 cctacgcggt ccaggacgca ccccgcggcc tggcggatgc ctttcgcgtg ggggctgact
     3301 tcatagggca tgactccgtc tccctggttc tcggagacaa catcttctac ggccatgggc
     3361 tgccggaagt gcttcggaac gcttcccggc aggagggcgg ggccaccgtg ttcgggtact
     3421 acgtgaatga tcccgagcgc tacggagtca tcgagttcga ccaggcccgg agagtggtct
     3481 ccatcgaaga gaagcccgcc cgacccaagt ccaagtacgc ggtggttggg ctgtatttct
     3541 acgacaacca ggtggtccag attgcgcggg atctgaagcc gtccccccgc ggagagctcg
     3601 agatcacgga cgtcaatcgg cggtatctgg agatgggtca attgcgatgc gagcttatgg
     3661 gacgagggtt cgcctggctg gatactggca cgttttcctc cctggtggat gccacgctct
     3721 acatcaaagc cattgaggac cgccaggggc tcaaggtggc ctgcgtggag gagatcgcct
     3781 accagatggg ctacatcggg gcggcggagc ttcggtccct ggcggagccc ttgcgcaagt
     3841 ccggctatgg ggagtacctc cttcggctgc tggaacaggg cccgaccttg ggcgggtagc
     3901 ggcgtgccat tcacgttcag cccggcggcg cttcggggtc tcgtgatcgt ccagccccgg
     3961 atcttcccgg atcagcgggg cttctttctg gagtcctata aagcaagcga tttcgaacgc
     4021 gcgggtctgc cggaacgttt cgcgcaagac aaccattccc tttcggagcg cggcgtgctt
     4081 cggggcctcc actaccagct tcctccgttc gctcagggga agctggttcg ggtcctggaa
     4141 ggccgggtct gggacgtggc ggtggacatc cgaccggact caccgaccta cgcgaagtgg
     4201 tttgggctgg agctgtccgc ggaggatcac cggatgctgt acgtcccacc cgggttcgcc
     4261 cacggattcc tgacgctaag tccgcgggcg cagttcttct acaagtgcac ggcggagtat
     4321 cgccgggaga gcgaagccgg catccgctgg aacgatccgg cgatcggcat tgcgtggccc
     4381 gtgacggagg tgaccgtgtc cgaccgggac gaggggttgc cgcttctcaa ggatgccacc
     4441 gtgtttcccg cggggttgta tccgtgatct gggtggtggg agcccggggc atgcttggca
     4501 gg
//
LOCUS       DASRZS010000426         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2540995, whole genome shotgun sequence.
ACCESSION   DASRZS010000426 DASRZS010000000
VERSION     DASRZS010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2540995"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VFH83_10425"
     CDS             complement(<1..537)
                     /locus_tag="VFH83_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HET6486825.1"
                     /translation="MRKPDHYWKLSKEEGYPARSVFKLKEIQERHHPIRPASRVLDLG
                     ASPGSWSLYVLELLTGGGGVTGVDLNAPDARLASRRRYRFIQGDFTDPAVLSQIQSLG
                     PFDVVLSDAAPSTTGTRALDTVRSLEIARQVLSICERCLNPRGSCVLKIFQGGAEREI
                     LDRMKVLFATARAFKPKAS"
     gene            664..1299
                     /locus_tag="VFH83_10430"
     CDS             664..1299
                     /locus_tag="VFH83_10430"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET6486826.1"
                     /translation="MEGFLRRPIRYRYYNATIILVVLCVLVFLVGLIDRRGFLIYLAL
                     IPDYVLQAGAWWQVATYLFVHSGWSHIFFNMLALFLFGIQLERRMGSTEFLLFYFFAG
                     IGAGVSTVLINAATGQGMIPVVGASGAIYGLLLAYASFFPDARIYVFGILPIRAPLLV
                     VVYAAIEIFSTFTGFQSGVAHLAHLAGLAFGYLYLVVRFGMNPVSIFLRSR"
     gene            1323..2009
                     /locus_tag="VFH83_10435"
     CDS             1323..2009
                     /locus_tag="VFH83_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486827.1"
                     /translation="MRVFLISLAALLAAGAVSAQAASAQAADRVFVRPWVELEPVVRI
                     EPGQYPLPLSAAERSLLDEARLLVSAMVYGWSFRFTPGDTSRAVAEQFVLTPVAEVPF
                     GNPRLRVIETELSDDHTRLWARVLYSLTDDETRRREAWESNTATLSTGQGEASVMKGA
                     SAKRASLEDAVRQAIQQGLHDRYLNKPREVDGQVVLWEDPQTRVGSGSYRTVATVKFL
                     LAEIVPYRIF"
     gene            2032..3696
                     /locus_tag="VFH83_10440"
     CDS             2032..3696
                     /locus_tag="VFH83_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6486828.1"
                     /translation="MLRKAIRVALPLVVAAAASVFTAGSAFAASAPAAAGTPAAASPS
                     SDSVREFVVAYEPQDVNLDPLHTFTSIESQFYTAIYEGLMVADPSTLEPTAGVAARWD
                     TTQGGKLYRFTLRSDARYSNGDPVRAQDFVNAWMRMIDPANNAEYSFLFDIIKGAHAY
                     RTGQSRDRSSVGIKAVSDSVLQVELEQPAAYFLKLLTHIAFLPLHPSLVTRTDWTNAS
                     TVIGNGPFLIASKSDQEIVLDKNPYYWDADNLGIDRLRIRFIEDKTKATEQYTAGHID
                     WVTSGDIDTSKLGTSDRVEAFPMFGTTYFFFACDAAPWSDWRVRRALALLVPWDKVRT
                     SDYVFPTDQMVPTIPGYPDVQGIDAQKVDEANRLLSDAGFPGGKGLPPLVVKLAKDSG
                     ASDLVNTMGDAWKAQIGLNVVIHEVDPNTYFDATRTRDYGMGISTWIGDYADPLTFLQ
                     LWTSDSNLNDAHFADPGYDAAVTASSGITNPGDRYKKLADAETLLLGHAAVLPLYHRA
                     AINLIALDRIGGWYSNPLDVHPFKFIKFKARATPPGITWAKDTAHG"
     gene            complement(3715..>3931)
                     /locus_tag="VFH83_10445"
     CDS             complement(3715..>3931)
                     /locus_tag="VFH83_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486829.1"
                     /translation="SPFGFWFFLAASTLGRLPGIAGSSIIGNAAATQRWTVLVIVSLA
                     ALALFVAGVILRPRIQAWLEKIAGRRP"
BASE COUNT          646 a         1330 c         1273 g          682 t
ORIGIN      
        1 gctggccttg ggcttgaacg cgcgcgccgt ggcgaagagg accttcatcc gatccaggat
       61 ctcgcgctcc gcgccgccct ggaagatctt cagcacgcag cttccgcgcg gattcaggca
      121 ccgctcgcag atcgacagca cctgccgggc gatctccagg gagcgcaccg tgtcgagcgc
      181 ccgcgtgccc gtggtggatg gggccgcgtc ggacaagacc acgtcgaacg gccccaggga
      241 ttggatctgg gagagcacgg cgggatccgt gaaatcgccc tggatgaacc ggtagcggcg
      301 ccgggacgcc agcctggcgt ccggcgcgtt gaggtccact ccggtcacgc caccgcctcc
      361 cgtcaggagc tccagcacgt agaggctcca gctcccggga gaggcgccca ggtccaggac
      421 gcgcgaggcc ggccggatag ggtgatggcg ctcctggatc tctttcagct tgaacacgga
      481 gcgcgccgga tacccttcct ccttggacag cttccagtag tggtccggct tcctcacggt
      541 cgggacgata ccagaggtgg cggggcggcg caaccgcggg cgcgcctcag cagcctccgc
      601 cccgctgggg caccccggtc ccgtgttgtt gactcacacc ctgaggcggc gtactttgag
      661 cccatggaag gcttcctgcg ccgaccgatt cgctaccgct actacaacgc caccatcatc
      721 ctggtggtgc tgtgcgttct tgtcttcctg gtgggtctga tcgatcgccg cgggttcctc
      781 atctacctgg ccctgatccc ggactacgtg ctgcaagcgg gcgcctggtg gcaggtggcg
      841 acctatctct tcgttcacag cggctggtcc cacatctttt tcaacatgct ggccctcttc
      901 ctcttcggga tccagctcga gcggcggatg gggagcaccg agttcctcct cttctacttt
      961 tttgcgggca tcggagccgg tgtctccacg gtgctcatca acgcggcaac ggggcagggc
     1021 atgataccgg tggtcggagc ctccggtgcc atctacgggc tgctccttgc ctacgcctcc
     1081 ttctttccag atgccaggat ctacgtcttc gggatcctgc ccatccgcgc tcccctcctg
     1141 gtggtggtct acgccgccat cgagatcttc tccaccttca cgggcttcca gtccggagtg
     1201 gcgcacctgg ctcacctggc gggccttgcc ttcgggtatc tctacctggt ggtccgcttc
     1261 ggcatgaatc cggtctcgat cttcttgcga agccgataac acccgatact cgtatcagag
     1321 agatgcgcgt cttcctgatc agcctcgcgg ccctgctcgc ggccggcgcc gtgtcggcgc
     1381 aagcggcttc ggcgcaggcc gcggaccggg tctttgtccg gccctgggtc gagctggagc
     1441 cggtggtccg catcgagccg ggccagtacc ccctccccct ttctgcggcg gagcggtctc
     1501 tcctggacga ggcccggctc ctggtctccg cgatggtcta cggctggagc ttccgcttca
     1561 ctcccgggga cacgagccgc gccgtggcgg agcaattcgt tctcacgccg gtggcggagg
     1621 tcccgttcgg caacccgcgc ctgcgggtca tcgaaaccga gctgagcgac gaccacaccc
     1681 ggctctgggc gcgcgtcctg tactccctga cggacgatga gacccgcagg cgggaggcgt
     1741 gggagagcaa caccgccacg ctgtccacgg gccagggcga ggccagcgtg atgaagggag
     1801 cctcggccaa gcgcgcctcc ctggaggatg ccgtccgcca ggcgatccag caggggctgc
     1861 acgaccgcta tctgaacaag ccccgcgagg tcgacggcca ggtggtcctg tgggaggatc
     1921 cgcagacgcg ggtgggctcg ggcagctaca ggaccgttgc cacggtgaag ttccttctcg
     1981 cggagatcgt cccctataga attttctgaa tcgaaagaat accttcaatc catgctgaga
     2041 aaagccattc gcgtcgccct gcctctcgtt gtggcggctg ccgcgagtgt gttcaccgcg
     2101 gggagcgcgt tcgcggcaag cgcgcctgca gccgcaggca cgcccgccgc cgcctcccct
     2161 agtagcgact ccgttcgcga gttcgtggtg gcgtacgagc cccaggacgt gaacctggac
     2221 cccctgcaca ccttcacgtc cattgaatcc cagttctaca cggccatcta cgaaggcctg
     2281 atggtggcgg acccgtcgac gctggagccg acggccggag tggccgcccg ctgggacacc
     2341 acgcagggcg ggaagctcta ccgcttcacg ctgaggagcg atgcccggta ctccaacggg
     2401 gatccggtgc gcgcccagga cttcgtgaac gcgtggatgc ggatgatcga tccggccaac
     2461 aacgccgagt actcgtttct tttcgacatc atcaaggggg cgcacgccta ccggaccggt
     2521 cagtccaggg accgctccag cgtgggcatc aaggcggtgt cggactcggt gctccaggtg
     2581 gagctggagc agccggcagc ctacttcctg aagctcctca cgcacatcgc ctttcttcca
     2641 ttacacccat cgctggtgac ccggaccgac tggaccaacg cgtccacggt gatcggcaac
     2701 gggcccttcc tgatcgccag caagagcgac caggagatcg tgctggacaa gaacccgtac
     2761 tactgggacg cggacaacct gggcattgac cggcttcgca tccggttcat cgaggacaag
     2821 accaaggcca cggagcagta caccgcgggg cacatcgact gggtcacctc cggggacatc
     2881 gacacgagca agctggggac ttcggatcgg gtcgaggcgt tcccgatgtt cgggaccacc
     2941 tacttcttct tcgcctgtga cgcggcgccg tggtccgact ggcgtgtccg gcgcgcgctg
     3001 gcgctgctgg tgccgtggga caaggtgcgc acctcggact acgtgttccc cacggaccag
     3061 atggttccca ccatccccgg gtatccggac gtgcagggca tcgacgcgca gaaggtcgac
     3121 gaggccaaca ggctcctctc cgacgctggc tttccgggcg gcaaggggct tccgcctctg
     3181 gtggtcaagc tggccaagga ttccggcgcc agcgacctgg tgaataccat gggagacgcc
     3241 tggaaggccc agatcggtct caacgtggtc atccacgagg tggatccgaa cacctacttc
     3301 gacgctacgc ggacgcggga ctacggcatg ggcatctcca cctggatcgg agactatgcg
     3361 gacccgctca cgttcctcca gctatggacc tcggacagca acctcaacga cgcgcacttc
     3421 gccgacccgg gctacgacgc ggcggtgact gcctccagcg ggatcaccaa tcccggggac
     3481 cgctacaaga agctggcgga cgcggagacg ctcctcctgg gccacgccgc ggttctgccc
     3541 ctgtaccatc gcgccgccat caacctgatt gccctggacc ggatcggggg atggtattcg
     3601 aacccgctgg atgtgcatcc cttcaagttc atcaagttca aggcccgagc gacgcccccg
     3661 gggatcacct gggcgaagga cacggcgcac ggttaggacg ggcaggacct gagcctaggg
     3721 acggcgtccg gcgatcttct cgagccaggc ctggatgcgg ggccggagga tcacgccggc
     3781 cacgaatagc gcgagggccg ccagggagac gatcaccagg accgtccagc gctgggttgc
     3841 cgccgcgttg ccgatgatgg agctgcccgc gatcccgggc agcctgccga gcgtggaggc
     3901 ggcgaggaaa aaccagaagc cgaacgggga c
//
LOCUS       DASRZS010000427         3953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2541022, whole genome shotgun sequence.
ACCESSION   DASRZS010000427 DASRZS010000000
VERSION     DASRZS010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2541022"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1544
                     /locus_tag="VFH83_10450"
     CDS             <1..1544
                     /locus_tag="VFH83_10450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6486830.1"
                     /translation="LVSADSGTRRLIEGSLYDITEQLLSEERIQDVARFPQEAPNPVM
                     RTTPEGELMYFNPASSALLPSWTADSPPRAPGESLAEIRAAWETGERRVLETREGKRT
                     FEIVVTPIRNRGYVNLYGREVTEEKALSGRLLQAQKMEAIGRLAGGVAHDFNNLLTVI
                     SGYCSLLEERLPEGSSERDDLAMISRSAERAATLTGSLLAFSRKQVMVPRIVNPGALV
                     KDMERMLTRLVGEDVEIRTFAHPDTGNIRVDPGQIEQVVMNLAANARDAMPRGGKLTI
                     ETGNRSLDEVYAREHPEVTAGEYVQVVVSDTGEGMDREVLQHLFEPFFTTKATGKGTG
                     LGLAMVYGIVKQSGGHVTCYSELGRGTTFMLYFPRTVEDVSAVAPAAERPASPGRGET
                     ILLVEDEESVRRFTEAVLKGKGYRVVAASTAAEALTYMGSPHDEVALLVTDVVMPRMS
                     GKDLAERLALTWPRLRVLYLSGYTGNVIVHHGVLDSGIDFLQKPFRVNDLLVKVREIL
                     DRAFA"
     gene            1541..2320
                     /locus_tag="VFH83_10455"
     CDS             1541..2320
                     /locus_tag="VFH83_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET6486831.1"
                     /translation="MKRAFLAAALLGLGSVLGFPQQTVDVLTTEYEPFCGVHGDTMWC
                     DLVNAAFAREGVTVRWQSFPQDREKAMVSDGQNVAFLTGTLVIDPAERQNFVVNPEPL
                     IYASIVAFYPREKYPQGLALRSPGDLKGRTVGVVQGTASVSVLGKAGVDIDVTTDKDQ
                     LIKKLVAGREDIAVIADLTGLGAFKAFFPGQIAAYGYELVYSSPIDLVFSKKNPRSRE
                     LLNRYNSGISKIKADGTFMEILGRYYPSGLINRSILPRDMR"
     gene            complement(2387..3274)
                     /locus_tag="VFH83_10460"
     CDS             complement(2387..3274)
                     /locus_tag="VFH83_10460"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET6486832.1"
                     /translation="MLVVVFGATGNTGREVCRSLSQKADVRIRAVSRNPSGASGLPAH
                     EVRAVTWTERSQLRSALEGADAAYFMLPTGLHNGSLFEFRDAYSRSFVELANDVKLKR
                     VVSLSSFGAHLAEKSGVLKGLCQAEKNLQEFAGEAVIIRPGYFWQNFAGNVSGIKEKG
                     FFGGYPVDLDVSLLFAHTADIGSAAAELLTAAALPFHRVLYVGHRERATFREVSSLIA
                     EKSGRKGLMWTNVGYDAARSFMLGRGMPAEIVGNLIEFFEAINTGRAMDDFTPDRLIP
                     TPIGKAEFAAWFAGQIGSG"
     gene            complement(3376..3594)
                     /locus_tag="VFH83_10465"
     CDS             complement(3376..3594)
                     /locus_tag="VFH83_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486833.1"
                     /translation="MMDSADELEKELAASRVTRVPSSVKSHWKGCKSCRWQFVECKDG
                     SLYRAQISKLARSPCASWTGSGLQDLPE"
     gene            complement(3661..>3953)
                     /locus_tag="VFH83_10470"
     CDS             complement(3661..>3953)
                     /locus_tag="VFH83_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6486834.1"
                     /translation="SQGGQRWVSLGLPGGGASITLLQAADTENLRAGTQKLYFTTRDA
                     KSLWQAVKARGITPTHDWTTETWAGSPWASWFSLADPDGNQVIIAQMKPSAE"
BASE COUNT          720 a         1266 c         1287 g          680 t
ORIGIN      
        1 acctcgtctc ggcggattcc ggcacacgcc gcctgatcga gggttccctc tacgatatta
       61 cggagcaact gctgtcggaa gagcggatcc aggacgtggc ccgcttcccc caggaggccc
      121 ccaatcccgt catgcggacg acaccggagg gagagctgat gtatttcaac cctgccagct
      181 cggcccttct tccttcgtgg acagcggatt cgccgccccg agctcccggg gaatcactcg
      241 ccgagatccg ggcggcctgg gaaaccggag aaagacgcgt cctggagaca cgcgagggca
      301 agcgcacctt tgagatcgtg gtgactccca tccgcaatcg cggctacgtc aacctctacg
      361 gtagagaggt cacggaggag aaggccctgt cggggaggct gctgcaggcc cagaagatgg
      421 aagccatcgg acggctcgcg gggggtgtgg cgcacgattt caacaacctg ctcacggtca
      481 tcagcggcta ttgcagtctc ctggaggagc gtctgccgga gggcagctcc gagcgggacg
      541 accttgccat gatctcccgt tcggcagagc gggccgccac gttgaccggc agcctcctgg
      601 cgttcagccg aaagcaggtc atggtgcccc ggattgtgaa ccccggcgcg ctcgtgaagg
      661 acatggagcg gatgctcacg cgcctcgtcg gtgaggacgt cgagatccgc acgtttgcgc
      721 atccggacac gggcaacatc agggtggacc cgggccagat agagcaggtc gtgatgaatc
      781 tcgccgccaa cgcgcgggac gccatgcctc ggggcggcaa gctgaccatc gagaccggca
      841 accgcagcct ggacgaggtg tatgcgcggg aacatcccga agtgacggcc ggcgaatacg
      901 tgcaggtagt cgtcagcgac acgggcgaag gcatggatcg agaggtgctg cagcacctgt
      961 ttgagccctt cttcactacc aaggccacgg ggaaaggcac ggggctgggc ctggcgatgg
     1021 tgtacggcat cgtcaagcag agcggcggac acgtgacctg ctacagtgag ctcggccgcg
     1081 ggaccacctt catgctttac ttcccccgca ccgtagaaga cgtcagcgcg gttgcccccg
     1141 cggcggaacg gcccgcctcg ccgggtcggg gcgagacgat cctgctcgtg gaggatgagg
     1201 aaagcgtgcg ccgcttcacc gaggcggtcc tgaagggcaa gggctaccgg gtagtggcag
     1261 cgtccactgc cgcggaggcg ctgacctaca tgggatcacc tcatgacgag gtcgcgctgc
     1321 tcgtcaccga cgtcgtgatg cccagaatga gcggcaagga tctcgcggag cgcctggcgc
     1381 tcacctggcc acggcttcga gtattgtacc tgtccggata caccgggaac gtgatcgtcc
     1441 accacggtgt gctcgattcc gggatcgact tcctgcagaa gccgttccgc gtcaacgacc
     1501 tgctcgtgaa ggtccgcgag atactggacc gggcattcgc atgaagcggg cgttcctcgc
     1561 ggcagccctg ctggggctcg gttccgtgct gggcttcccg cagcagacgg tggacgtgct
     1621 gaccaccgag tacgagcctt tctgcggcgt gcacggggac acgatgtggt gcgacctggt
     1681 gaacgccgcg ttcgcgaggg aaggcgtcac ggtccggtgg caaagctttc cccaggaccg
     1741 ggaaaaggcc atggtgtcag atggccagaa cgtcgcgttc ctgaccggga ccctggtgat
     1801 tgacccggcg gaacggcaga acttcgtcgt gaatcctgaa cccctgatct acgcgagcat
     1861 cgtggcgttc tatccacgag agaagtaccc ccagggcctg gcgctgagaa gccccggtga
     1921 cctgaaaggt cggacggttg gcgtggtcca gggcacggcg tcggtctccg tccttgggaa
     1981 agccggagtg gacatcgatg tgaccacgga caaggaccag ctcatcaaga agctggtggc
     2041 aggacgagaa gacatcgccg tgatcgcgga cctgaccggc ctgggggcgt tcaaggcgtt
     2101 ctttccgggg cagatcgccg cctacggcta tgagctcgtc tacagctctc cgatcgatct
     2161 tgtgttctca aagaagaatc cgcggtcccg ggagctgttg aacaggtaca attcggggat
     2221 ttcaaagatc aaggcagacg ggacgttcat ggagatcctc ggaaggtact acccgagcgg
     2281 cctgatcaac cgcagcatcc tgcccaggga catgcggtag cgcatcccgc ggttgcggga
     2341 gaatcgacgc gcgccatacc ggtccctcgc ggcccgagca agctccctac cccgagccga
     2401 tctgcccggc aaaccaggcg gcgaactcgg ccttgccgat cggcgtcggg atgagacggt
     2461 ccggcgtgaa atcgtccatg gcgcggccgg tgttgattgc ctcgaagaac tcgatgaggt
     2521 tgcccacgat ctccgccggc atcccccgcc cgagcatgaa cgaacgcgcg gcatcgtagc
     2581 cgacgttcgt ccacatcagg cccttgcggc ccgacttctc cgcaatgagg ctgctgacct
     2641 cgcggaaggt ggcgcgctcc cgatggccga cgtacaggac gcggtggaag ggcagcgctg
     2701 ccgcggtcag gagctctgcc gcggcgctgc caatgtccgc ggtgtgcgca aagagcaggc
     2761 tcacatcgag gtcaacgggg taaccgccga agaagccctt ctccttgatg ccggacacgt
     2821 tgccggcaaa gttctgccag aagtaaccgg gacggatgat cacggcctct cccgcaaact
     2881 cctggaggtt cttctccgcc tggcagaggc ccttgagcac gccgcttttc tccgccaggt
     2941 gcgcgccgaa gctgctcagg gacacgaccc gcttgagctt cacgtcattt gcaagctcga
     3001 cgaacgagcg ggaatacgca tctcgaaact cgaacaggga gccgttgtgc agcccggttg
     3061 gcagcatgaa atacgcggca tccgcgccct cgagcgcgga ccgtagctgg gatctctcgg
     3121 tccaggtcac cgctctcacc tcgtgggccg gcagtccgct ggcaccggaa gggttgcggc
     3181 tcaccgcgcg gattcgaacg tccgcctttt gcgaaaggct ccggcagact tccctgcccg
     3241 tgttccccgt tgcgccaaag actacaacaa gcatggattc gttcccccgt ggcagccgag
     3301 cctgcctgcc ctaatctacg aaactcctgc ccggtggtcg aggattgggg ggggatccga
     3361 gagctgccgg ggcggctact ccggcagatc ctggagaccg gaaccggtcc aggaggcgca
     3421 cggtgagcgc gccagcttgc tgatctgcgc gcggtagagg ctgccgtcct tgcactccac
     3481 gaactgccag cggcagctct tgcaaccctt ccagtgcgat ttcactgagc tcgggacgcg
     3541 ggtcacgcgg cttgctgcca gttccttttc aagctcatca gcgctgtcca tcatggtgcg
     3601 ttccttcgca gagccccata ctaactcgcg ccagccgcgt acacaacccg cgtcggaggc
     3661 ctactccgct gaaggcttca tctgggcgat gatcacctgg ttcccatccg gatccgccag
     3721 gctgaaccag gatgcccagg gcgagcccgc ccacgtttcc gtggtccaat catgcgtggg
     3781 cgtgatgccc cgtgccttca ccgcctgcca gagtgacttc gcgtctcggg tcgtgaagta
     3841 aagcttctgc gtcccggctc gcaggttttc cgtgtctgcc gcctgcagca gcgtgatcga
     3901 ggcccctccg ccgggcaggc ccagggagac ccaccgctgt cctccctggc tga
//
LOCUS       DASRZS010000428         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2541436, whole genome shotgun sequence.
ACCESSION   DASRZS010000428 DASRZS010000000
VERSION     DASRZS010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2541436"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFH83_10475"
     CDS             complement(<1..385)
                     /locus_tag="VFH83_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET6486835.1"
                     /translation="MKRVSVVLLAILFLAPAVRALAASKVKGISSAEVCGECHKEIYR
                     MWKTSIHSQAMEDNVFQNAYHETESSQGASVSRVCLRCHAPLTDINHDFKLGQKVTWE
                     GINCDVCHSIVSVEVAGGSARQTLDI"
     gene            complement(613..1542)
                     /locus_tag="VFH83_10480"
     CDS             complement(613..1542)
                     /locus_tag="VFH83_10480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET6486836.1"
                     /translation="MGIRPLLRILLGWFSAMLAENVVGTILIVAYALATGGAARLHQI
                     TQTGGTALAPYALVLLTGAASPAVWLWVWFYRRRVDKGSFLDLGLRGRGRAVRFAVGW
                     FCGLVAPGLVVAAGLLTRCYLWGGAALNEGAGPETTLGAWIVLLPLPFIVQGSAEELL
                     ERGYLQRSLWEWRSGTPHRVAWALLVPSLLFSLSHLANPGFGPIPFVNTVLIGILFAC
                     FTLSTGSLWLAIGIHGGWNFGLSVVWSLPVSGVRLMHILPVRIAPGASVLFGGEYGPE
                     GGLAVTLLVIALLSWALPRALDVAAGDGHVSSG"
     gene            complement(1561..>2021)
                     /locus_tag="VFH83_10485"
     CDS             complement(1561..>2021)
                     /locus_tag="VFH83_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013180870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase VI"
                     /protein_id="HET6486837.1"
                     /translation="LVSMGGVPTRATRRFIRRLADEKKIPVYAFVDCDPYGISNIYRT
                     LKVGSGNAAHINQFFCVPQARFLGVTPQDIVDYKLPTHPLKDVDVKRAQDAIKNDPFF
                     QQHKPWQKAMDQLLKMGVRAEQQALAKWGLNYVIEKYLPAKLAAAERFLP"
BASE COUNT          402 a          637 c          667 g          315 t
ORIGIN      
        1 cgatgtccag ggtctgcctg gccgagccgc cggcgacctc caccgagacg atggagtggc
       61 agacatcgca gttgatccct tcccaggtga ccttctgtcc cagcttgaaa tcgtgattga
      121 tgtccgtcag cggcgcgtgg cagcggaggc agacccggga cacggacgcg ccttgactgg
      181 attcggtctc atgataggcg ttctggaaga cgttgtcctc catcgcctgg gaatggatgg
      241 aggtcttcca catgcggtag atctccttgt gacactcacc acagacctcg gcgctggaga
      301 tgcccttgac cttgctggcg gcgagagccc gcacggccgg agcgaggaag agaatagcca
      361 ataggaccac agagacacgc ttcatctgga gtctccttgc acaagtcatg cggccgcggc
      421 cgtttgcgcc ggatcagcgt aggtcggatt cggagcgctc acaacaagaa catgcctgta
      481 ttcatatttt gtattccaag gagatgaaaa aggcgccgcc cggtcccgac gatggctgcg
      541 taatcacaag ttgtcacggg atttggtgcg cgcgccgccc gcgtgtgcca gcagccggag
      601 ttctcccgtg cctcaaccgg aactgacgtg cccatccccg gcggcgacgt cgagcgcccg
      661 gggcagcgcc caggagagca gcgcgatcac cagcagggtg acggcgagtc ctccttccgg
      721 gccgtactct ccaccgaata ggaccgaggc acctggagca atccgcacgg gcaggatgtg
      781 catgagacgc actcccgata ccgggagcga ccagaccacg ctcagcccga agttccatcc
      841 cccgtgaatc ccgatggcca accagagcga accggtggaa agggtgaaac aggcgaagag
      901 gatcccgatg aggacggtgt tgacgaaggg gatcggtcca aagcccggat tggccagatg
      961 ggagagggag aagagcagcg acggaaccag aagagcccag gccacgcggt gaggcgtgcc
     1021 cgagcgccac tcccagaggc tgcgctgcag ataaccgcgt tcgagcagct cctcggccga
     1081 tccctggacg atgaacggca gagggagcag gacgatccag gcacccaggg ttgtctccgg
     1141 tccggcacct tcattgaggg cggcgccacc ccagaggtag cagcgggtca gcagtccggc
     1201 ggccacgacc aatcccgggg cgaccaggcc gcagaaccag cccacggcga agcggacggc
     1261 ccgcccgcga cctcgcagtc ccagatcgag gaagcttccc ttgtcgactc gccggcgata
     1321 gaaccagacc cacaaccaga cggcgggcga ggccgctccg gtcaggagca cgagcgcata
     1381 gggtgcgaga gcggtgcctc ccgtctgggt gatctggtgc agccgggccg cgccgccggt
     1441 ggccagggcg taggcgacga tcaggatcgt ccccacgaca ttctccgcca gcatggccga
     1501 gaaccaacca aggagaatgc gcaggagcgg ccggatgccc atcaggattc cgccggcgga
     1561 tcagggcagg aaacgctccg ccgccgccag cttggccggc aggtacttct cgatgacata
     1621 gttcaacccc cacttggcca gcgcctgctg ctcggcgcgc acccccatct tcaacagctg
     1681 gtccatcgcc ttctgccacg gcttgtgctg ctggaagaac ggatcgttct tgatcgcgtc
     1741 ctgggcccgt ttcacgtcca cgtccttgag cgggtgggtg gggagcttgt agtcgacgat
     1801 gtcctggggg gtcactccca ggaagcgggc ctggggtaca cagaagaact ggttgatgtg
     1861 ggccgcgttg ccgctcccga ccttcagcgt gcggtagata ttggaaatgc cgtagggatc
     1921 gcagtcgacg aaggcgtaga ccggaatctt cttctcgtcg gccagccggc ggatgaaacg
     1981 acgcgtggcc cgggtgggca ccccccccat ggagacgagg a
//
LOCUS       DASRZS010000429         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_621385, whole genome shotgun sequence.
ACCESSION   DASRZS010000429 DASRZS010000000
VERSION     DASRZS010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621385"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VFH83_10490"
     CDS             <1..552
                     /locus_tag="VFH83_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486838.1"
                     /translation="YSFGGGIAATLAPLLPSLSRLVLLSPALVGYPYRHGMLLPLIAV
                     EALSALREAVQRRRVAAYVAIGYRFMATFVRHPRTQYRTFRVILRSFLRCRPDGLSLP
                     DTSIISAARDRFFPVAGGKDLARAVPHASLEVIGGIHLWPLLEHDKAMAVIHEHLRRP
                     ASPAARAGRASHAHRQALSGAPR"
     gene            complement(533..994)
                     /locus_tag="VFH83_10495"
     CDS             complement(533..994)
                     /locus_tag="VFH83_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486839.1"
                     /translation="MGEFSFLGLNVLWSFLVLIRAIEQNLPSLDELFASDFPVPRDRS
                     GVSRRKALALLERIRPVLERVLNLRRDPGSPLLGILTREARRSLWDGQLHLPEEIRGP
                     ASDLLDSCVRAARFFESDPKAYGDCLAKIRDDYARLVSRVRDTYYITSEPR"
     gene            complement(1059..2012)
                     /locus_tag="VFH83_10500"
     CDS             complement(1059..2012)
                     /locus_tag="VFH83_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET6486840.1"
                     /translation="MANGGERERLPREVWIATLTQEGIEGTSCQEITEKALRKMEGFL
                     PQRPDIFCLPETFHYFAFPGARPAVQELVDTGEAPIIEAMATFARRNRCYVIAPILTG
                     EAGACYNAAVLIDRTGTRMGEYRKVRTTEDEMDCGVSPGPVGPTVFETDFGRIGIQIC
                     YDIEWPQGWKALHDQGAEIVFWASAFGGGKRIGGLAWMNRYCVVSSTAKGASRICDMT
                     GETIAESGLWDPNGVCAGLNLEKAFIHTWPYVEKFGAVRGKYGRSIRIYSLHEEEFSV
                     IESRSADVRVADVMREFGIPSYDEHLRRAEKRHRVKDLAEA"
     gene            2249..2827
                     /locus_tag="VFH83_10505"
     CDS             2249..2827
                     /locus_tag="VFH83_10505"
                     /inference="COORDINATES: protein motif:HMM:NF037152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6486841.1"
                     /translation="MKFESQKSQILIAAESVVHRFGYAKTTMTDIARALRKGKSSLYH
                     YFTSKEQIFVALLHKEIAELRGEFLKAVDAESTPERKIRAYILTRTRMFRDKLNQHLS
                     FMEETPERFELLTRIHAEYDADEVAIISGILEAGVAEGRFVIDDVPATATAIVHVLKG
                     FELPFVSANSAAEVEAKLDRTLRILFYGIIPR"
BASE COUNT          509 a         1005 c          924 g          559 t
ORIGIN      
        1 tactcgttcg gaggaggcat cgcggccact ctggcgcccc tccttccgtc cctgagtcgc
       61 ctggtgctcc tgagtccggc gctggtcggt tatccctacc gtcacgggat gctcttgccg
      121 ctcatcgccg tggaagcgct ctccgcgctt cgggaggcgg tccagaggcg gcgcgtcgcg
      181 gcgtacgtcg cgatcggata ccggttcatg gctaccttcg tccgccatcc ccgcacgcag
      241 taccgaacct tccgcgtgat cctccgatcg ttcctccggt gcaggccgga tggcctctcg
      301 ctgccggaca ccagcatcat ctccgcggcc cgggatcgtt tctttccggt tgccggcggg
      361 aaggatctgg ccagggccgt gcctcacgct tctctggaag tcatcggggg cattcacctc
      421 tggcccctcc tggagcatga caaggccatg gccgtaatcc acgagcatct tcgccggccg
      481 gcctcaccgg ccgccagggc cgggcgcgcc tctcatgccc atcggcaggc gctcagcggg
      541 gctccgaggt gatgtagtac gtatcccgga cccgggatac cagtcgcgcg tagtcgtccc
      601 ggatcttggc aaggcagtct ccataggcct tgggatccga ctcgaagaag cgggcggccc
      661 ggacgcagga atccagcaga tcggatgcgg gaccccggat ctcctccggg agatgaagct
      721 ggccgtccca aagggacctg cgggcttccc tggtcagtat ccccagcaga ggggagccgg
      781 ggtcccggcg caggttcagg accctttcca gcactggtcg gatccgctcc agcagggcca
      841 gcgcctttcg ccgggagacc ccgctccggt ccctcggcac cgggaagtcg gacgcgaaca
      901 gctcatcgag actcggcagg ttctgctcga tcgcgcggat gaggacaagg aaactccaca
      961 gtacgttcaa acccaggaag gagaactcgc ccacatcgac ccaaggaaag gccatggtcc
     1021 tgaccccagt ggttgccagg acaatggtac tccctgtgtc aagcttcggc gaggtctttg
     1081 acgcggtggc gcttctccgc gcggcgcagg tgctcgtcgt agctggggat cccgaactcc
     1141 cgcatcacgt ccgccacccg cacgtccgcg gatcggcttt cgatgaccga gaactcctcc
     1201 tcgtgcaggg agtagatccg aatgctccgt ccgtactttc cccgcaccgc gccgaacttc
     1261 tccacgtacg gccaagtgtg gatgaacgcc ttctcgaggt tgaggccggc gcagactccg
     1321 ttggggtccc agaggccgga ctcggcaatc gtctcaccgg tcatgtcgca gatccgactc
     1381 gcccctttgg cggtgctcga aaccacgcag taccggttca tccacgcaag ccccccgatc
     1441 cgcttgccgc ccccgaaggc ggatgcccag aacacgatct ccgccccctg gtcgtggagg
     1501 gccttccatc cctgcggcca ttcgatgtcg tagcagatct ggatgccgat ccggccgaag
     1561 tcggtctcga acaccgtggg gcccaccggc ccagggctca cgccgcagtc catctcgtct
     1621 tcggtcgtcc ggaccttgcg atactcaccc atgcgcgtgc ccgtgcgatc gatcaagacc
     1681 gcggcgttgt agcaggctcc cgcctccccg gtgaggatcg gcgcgatcac gtagcagcgg
     1741 ttgcggcgcg cgaacgtcgc catggcctcg atgatcggcg cctccccggt gtccacgagc
     1801 tcctgaacgg ccggcctggc tcctgggaag gcgaagtaat ggaaggtttc cggaaggcag
     1861 aagatgtccg gccgctgcgg caggaatccc tccatcttgc gtagggcctt ctcggtgatc
     1921 tcctggcagc tcgtgccctc gattccttcc tgagtgaggg tggcaatcca cacctcccgg
     1981 ggaagtcgtt cccgctcgcc gccgttcgcc atgggacctc cttctgccgc atcgtcctta
     2041 gctatcgaag gatgcaacga cgtagtcatc cttttccgtg ctgtgcctga ccgtcaagtc
     2101 ggcggcaagc cgcgcagccc gcgcccggag ggtttccgca ccaggaagca tcgcctgcca
     2161 aggccttgac ccggcaactt gtctcatgga ctattcgacc aatagagcgt tttggtcgaa
     2221 tagtctcaag gaggctcgtg tgccggagat gaagttcgag agccagaaaa gccagatcct
     2281 gattgctgcc gagtcggtgg ttcaccgttt tgggtatgcc aagaccacga tgacggacat
     2341 cgcacgagcg ctgcgcaagg ggaagagctc cctctaccac tacttcacga gcaaggagca
     2401 gatcttcgtg gcgctgctcc acaaagagat cgcggagctg cgcggcgagt tcctgaaggc
     2461 cgtagatgca gaaagcacac cggagcgaaa gatccgagcc tacatcctca cccggacccg
     2521 gatgttccgg gacaagctca atcagcacct ctccttcatg gaggaaacgc cggagcgctt
     2581 cgagctcctg acccggatcc acgcggaata cgatgccgac gaggtggcca tcatctcggg
     2641 gatcctggag gcgggcgtgg ccgaggggcg cttcgtgatc gacgacgtcc cggccacggc
     2701 gacggcgatc gtccacgtct tgaagggttt cgagcttccc tttgtctccg ccaacagcgc
     2761 ggcggaggtc gaggcgaagc tggaccggac cctcaggatc ctcttctacg gcatcatccc
     2821 gcgctgagct tcggagagac gtccatgcag aagctgacgg gcttcgtctt ccggtggcgt
     2881 gtcccgatca tcatcgggtt cgccgtcatc acgctcttct tcggcttctg gatcaaggac
     2941 cttcgggtgg acagtgacgt gatcggctat cttccgcgcg aggacgaggc cgttcgg
//
LOCUS       DASRZS010000430         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_621788, whole genome shotgun sequence.
ACCESSION   DASRZS010000430 DASRZS010000000
VERSION     DASRZS010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621788"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..1640
                     /locus_tag="VFH83_10510"
     CDS             291..1640
                     /locus_tag="VFH83_10510"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET6486842.1"
                     /translation="MALKCAYIGAGSRTFGPSIIRDLFLSEPICSKGLELVLMDIVAD
                     HLGEIGRYCEFLNARLRRSVTVRLTTDLTEALRGADFVVCAIEVQRYLYWSQDFHVPR
                     DTGIRHPFGENGGVGGIFHALRNMGPMVHIAREMERLCPKALMLSFTNPEHKLCEGVT
                     RLTSIQAIGLCHGVFMGQEQIAEILGRPESELDTAACGINHFTWFQEIRDKKTGEDLY
                     PLLKERERKTDWFTKWHEMALGRILLRRFGMWPSPGANHYGEYIRWADEYVVSNLQFH
                     YDPEDGHPWTEDIEPRFVYYADNVDTSGPWGEPRKKDPGTPMEQQTLKASGEIAVKII
                     ESLATGVSNHLEAINVPNRGYIPNLDEGTVVEVPAHADGGGIKPRMMRPFPEAIAALL
                     RLQGSIHKLIVEAFAETSREKLIQAVLLDPSVDSYRHAIVAIERLLELQKNILPKFT"
     gene            1847..2023
                     /locus_tag="VFH83_10515"
     CDS             1847..2023
                     /locus_tag="VFH83_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486843.1"
                     /translation="MITEAGRIIVMEHSVVWGFDAATLEFFRAVRDGRTTRSSFQTSA
                     ETMRVAREIEAVRP"
     gene            2020..>2524
                     /locus_tag="VFH83_10520"
     CDS             2020..>2524
                     /locus_tag="VFH83_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486844.1"
                     /translation="MTAAREQPYEARPLVVSTGASQAIRVERTLDARRVRLGPSAVVE
                     VLERKSYRVREVVPASIRSAEDGLVSIEFRHTPREPGEYLIHVGADGRRWPAPSALEL
                     SFFALPQELASCRPFKGDMHMHTYHSDGIQSPVVMAARCRQVGLDFAAITDHRFYRSS
                     IEAMDRSR"
BASE COUNT          447 a          809 c          860 g          408 t
ORIGIN      
        1 ggtggcggat ccgttcctga acggtggaga cctggatccc atcgacgcgg gagcgcctcc
       61 gggaaccgcg gacgccaccg agcagaagat acggcgtgtg cttgatcggg tcgtcgaggt
      121 agacctggcg cccctggaag gacggcgggg gcctggacat cgaggcggcc ttgaccagat
      181 cctaccagga ccttaccaag tagcggctcg gcccttcggg gggagaggca acccgcggat
      241 cgtcgcggcg gccgtctccc cctgatggtt cccgacccaa ggaggacgca atggcgttga
      301 aatgcgcgta catcggcgct gggagccgaa cgttcgggcc cagcatcatc cgagacctct
      361 tcctgagcga gccgatctgc tccaagggcc tggagctggt gctcatggac atcgtggcgg
      421 atcatctggg ggagatcggt cggtattgtg agttcctcaa cgccaggctc cgccggtcgg
      481 tcacggtgcg cctgaccacg gacctgacgg aggcgcttcg cggagcggac ttcgtggtct
      541 gcgccatcga ggtgcagcgg tatctctact ggtcccagga cttccacgtt ccccgggaca
      601 cggggatccg ccatcccttc ggggagaacg gcggggtagg ggggatcttc cacgcgcttc
      661 gcaacatggg gcccatggtg cacatcgccc gggagatgga gcgcctctgc cccaaggccc
      721 tcatgctgag cttcacgaat ccggagcaca agctctgcga aggggttacg cggctgacct
      781 ccatccaggc catcgggctc tgccacggcg ttttcatggg gcaggagcag atcgcggaga
      841 tcctggggcg gccggagtcg gagctggata ccgccgcgtg cggcatcaac cacttcacct
      901 ggttccagga gatccgggac aagaagacgg gtgaggacct gtatccgctc ctgaaggagc
      961 gggagcgcaa gacagactgg ttcacgaagt ggcacgagat ggccctgggc cggatcctgc
     1021 ttcgacgctt cggcatgtgg ccctctccgg gggccaacca ttacggggag tacattcgct
     1081 gggccgacga gtacgtggtg tccaacctgc agttccacta cgatccggag gacggccacc
     1141 catggaccga ggacatcgag ccgcggttcg tgtactacgc ggacaacgtc gacacatccg
     1201 gtccctgggg tgagccgcgc aagaaggatc ccggaacgcc catggagcag cagacgctca
     1261 aggcatccgg agagatcgct gtaaagatca tcgagagtct ggccacggga gtctccaacc
     1321 acctggaagc catcaacgtc cccaaccgcg gttacatccc caacctggac gagggcaccg
     1381 tggtcgaggt ccccgcgcac gccgacggcg gggggatcaa gccgcgcatg atgcgcccct
     1441 tccccgaagc catcgcggcg ctccttcggc ttcagggcag catccacaag ctgatcgtgg
     1501 aggcattcgc ggagacctcc agggagaagc tcatccaggc ggtgctcctc gatccgtcgg
     1561 tggactcgta ccgccacgcc atcgtggcca tcgagcggct cctggagctt cagaagaaca
     1621 tcctgcccaa gttcacctga ggtccatgca gcccccgcgt cccgcggcca cgcctccgcg
     1681 gcccgccccc acgcctccgc ggccgacccg gcggacacag agctgtacgt ggcgaagggg
     1741 acggccgagc gtatctcttc tccttcgcgg atggcgtgga gaacgctcgg gaagtctgga
     1801 acgtgcgggt gtgggcaaag gagagctcac acggtatcgt gccaacgtga taaccgaagc
     1861 cggacggatc atcgtgatgg agcactcggt agtctggggc tttgacgcgg caaccctgga
     1921 gttcttccgg gcggtgcggg acggaaggac cacccggtcc agtttccaga cctcggcgga
     1981 gacaatgcgg gtggcccggg agatcgaggc ggtccgacca tgaccgcggc tcgggagcaa
     2041 ccgtacgagg ctcggccgct ggtggtctct accggagcct cgcaggccat ccgggtcgag
     2101 cggaccctgg atgccaggcg cgtgcgactg ggtccatcgg cggtcgtgga ggtcctggag
     2161 cgcaagagct atcgcgtccg tgaggtagtg ccagccagca tccgttcggc ggaggatggg
     2221 ctcgtctcca tcgagtttcg ccatacgccc cgggagccgg gcgagtatct catccacgtc
     2281 ggcgcggacg ggaggcggtg gcccgcgccc agcgccctcg agctttcgtt cttcgcgctg
     2341 ccgcaggagc tggcgtcctg tcgacccttc aagggcgaca tgcacatgca cacctaccac
     2401 tcggacggca tccagtctcc cgtggtcatg gcggcccggt gtcggcaggt ggggctggac
     2461 ttcgcggcaa tcacggacca ccgcttctac cgcagcagca tcgaggcgat ggatcgatcc
     2521 cggg
//
LOCUS       DASRZS010000431         2545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5622852, whole genome shotgun sequence.
ACCESSION   DASRZS010000431 DASRZS010000000
VERSION     DASRZS010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5622852"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFH83_10525"
     CDS             complement(<1..238)
                     /locus_tag="VFH83_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486845.1"
                     /translation="MQVVQECRVRDTVDALRRPEGAVILVQEVPPPDQTKPVGKVELA
                     PVPVSGAPGVTQAEADPLFSGLHDEIHLTQPSFLP"
     gene            384..1490
                     /gene="hisC"
                     /locus_tag="VFH83_10530"
     CDS             384..1490
                     /gene="hisC"
                     /locus_tag="VFH83_10530"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET6486846.1"
                     /translation="MKSTGEALDLVRRTIREMRGYVPGLQPSPGQRLVKLNTNENPYP
                     PSQSVLQALSGAVNDTLRLYPSPDAAPVRKEAAERYGISASQVLCGNGSDEILALIMR
                     AFVDEGDAIAYFEPSYSLYPVLAGIAGARTVVIPWARPREASDVGRTPIPTPRAKVFF
                     LTTPNSPYGSLFPTSWIRRLLDAFSGIVIADEAYVDFATESALPLLAEYPRLICVRSL
                     SKSYSLAGLRAGLALASEELIAQIAKVKDSYNVNRLSQAAAAAAIADEEYRRQTSERI
                     KATRARLSRRLTDLGLVIPPTQANFLFAALPNGSGTEKPDAGPLARFLLERGFLVRHF
                     DTPTLRDGMRISIGTDQEIDALADAIEEGLRGRQ"
     gene            1477..>2545
                     /locus_tag="VFH83_10535"
     CDS             1477..>2545
                     /locus_tag="VFH83_10535"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET6486847.1"
                     /translation="MGVSKRIRASLESSSWIRRMFEAGNELRRVHGPDAVCDFSLGNP
                     DVEPPAAFQTALQEVVSATIRRKHGYMPNAGFPEVRELVAGQVSKEQGVPLTGDSVVM
                     SCGASGAMNSALKALLDPGDEVVVSTPCFMEYAAYVDNHGGKLVFCESRPDFDLDVAS
                     LESKIGPRTAALIINSPNNPTGRIYSESTLRDLAQMLGRASARFGRAIFLLSDEPYRK
                     LAYDGVTVPGVLGLYPNSIVLSSSSKDLSLPGERIGYVAVNPAAEEARMLVDGIILAT
                     RILGYVNAPALMQRVVGKIQGASVEVDVYRRKRDRFVGALSGMGYRVASPQGAFYLFP
                     KAPGGDELPFVEALTEERILVV"
BASE COUNT          418 a          855 c          828 g          444 t
ORIGIN      
        1 gtggcagaaa ggaaggttgc gtcaggtgga tctcatcgtg gagaccgctg aagagcggat
       61 cggcttctgc ctgagtgaca cccggtgccc cagataccgg gactggcgcc agctctacct
      121 tgcccacggg cttggtctga tccggcgggg gtacctcctg caccaggatg accgcgcctt
      181 ccggccggcg gagggcatct acggtatccc gcacgcggca ttcctggacg acctgcacgg
      241 gtggatcacc tgcggcacga atacccggga ggctggatgg gccatccagc ggatcaacgc
      301 ccaggcggtg ctcgaaagcg tgcccgctgc gcgcgagctt cgcgagcttg ccggctcttg
      361 gcgcctttgt tacactgcgc cgcatgaaat caactgggga ggccttggac ctcgtccgcc
      421 ggacgatccg agagatgcgc ggctatgtgc cgggtctcca gccctccccg gggcagaggc
      481 tggtgaagct caacaccaac gagaacccgt atcccccttc ccagagcgtg ctccaggctc
      541 tgagcggggc ggtgaatgat acgcttcgtt tgtacccgag ccccgatgcc gcccccgttc
      601 gaaaggaggc ggcggagcgc tatggcatct cggcctccca ggtgctttgc gggaatggat
      661 cggatgagat cctcgccctc atcatgcgcg ccttcgtgga tgagggggac gccatcgcct
      721 acttcgagcc ttcatactcc ctctatccgg tgctggcagg cattgcgggg gcccggaccg
      781 tcgtgattcc ctgggcgcgt ccacgggagg cctcggacgt ggggcgcaca cccatcccca
      841 cgccccgtgc gaaggtgttc ttcctgacca cgccgaactc cccgtacggg agcctcttcc
      901 cgacctcctg gatccgtcgg cttctcgatg ctttctccgg gatcgtgatc gcggatgagg
      961 cgtacgtgga tttcgcgacg gagtccgcgc tgccgcttct ggcggagtac ccgcggctga
     1021 tctgcgtccg atcgctctcc aagtcctact ccctggccgg cttgcgcgcc ggcctggcgc
     1081 tggccagtga ggagctgatc gcccagatcg cgaaggtgaa ggacagctac aacgtcaacc
     1141 gtctgtccca ggccgcggcc gccgcagcca tagcggatga ggagtaccgg cgccagacct
     1201 cggagcggat caaggccacg cgcgccaggc tgtcgcggcg gctcacggac ctggggctcg
     1261 tgatcccgcc aacccaggcc aacttcctct tcgcggcgct gcccaacgga tccggcacgg
     1321 agaagccgga tgcgggaccc ctcgcccggt tccttctgga acggggcttc ctggtgcggc
     1381 atttcgatac cccgacgctt cgggacggga tgaggatcag catcggaacc gatcaggaaa
     1441 tcgatgcgct tgccgatgcc attgaagaag gcctccgtgg gcgtcagtaa gcgaatccgt
     1501 gccagcctcg aaagctcttc ctggatccga cggatgttcg aggcggggaa cgagcttcgt
     1561 cgcgtccacg gccccgatgc ggtttgcgac ttcagcctgg gaaatccgga cgtggagcca
     1621 ccggccgcct tccagaccgc cctccaggag gtcgtgtccg ccaccatccg gcgcaagcac
     1681 ggatacatgc ccaacgcggg gtttccggag gtccgagagc tcgttgcggg acaggtgagc
     1741 aaggagcaag gggtcccgct gaccggtgac tccgtcgtga tgtcctgcgg cgcaagcggc
     1801 gccatgaaca gcgcgctcaa ggcccttctg gaccccggcg acgaggttgt tgtcagcacg
     1861 ccgtgcttca tggagtacgc cgcctacgtg gacaaccatg gcggaaagct ggtgttctgt
     1921 gagagccgcc cggacttcga cctggacgtt gcctccctgg agtccaagat cggtccgcgc
     1981 accgcggcac tcatcatcaa ctcacccaac aacccgaccg gacggatcta ctccgagagc
     2041 acccttcgag acctagctca gatgctgggc cgcgcgagcg cccggtttgg acgcgccatc
     2101 ttcctgctct cggacgagcc ttaccgcaag ctcgcctatg acggcgtgac ggttccgggg
     2161 gtgctcggac tctaccccaa ctccatcgtg ctcagctcgt cctccaagga cctgtccctc
     2221 cccggggagc gcatcggcta cgtcgccgtg aaccccgccg cggaagaagc ccggatgctg
     2281 gtggacggga tcatcctggc tacgcgcatc ctggggtacg tgaacgcgcc ggccctcatg
     2341 cagcgcgtag tgggaaagat ccagggagcc agcgtagagg tcgacgtgta ccgccgcaag
     2401 cgggaccgct tcgtcggcgc cctgtccggc atgggctacc gggttgccag cccgcagggg
     2461 gcattctacc tgttcccgaa ggcccccggc ggggatgagc ttccgttcgt cgaggcgctc
     2521 acggaggagc gcatccttgt cgtgc
//
LOCUS       DASRZS010000432         5421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2542935, whole genome shotgun sequence.
ACCESSION   DASRZS010000432 DASRZS010000000
VERSION     DASRZS010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2542935"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFH83_10540"
     CDS             complement(<1..377)
                     /locus_tag="VFH83_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6486848.1"
                     /translation="MGARKKRVTIYDIASAAGVTAATVSRVLNGRHGISHATRERVIG
                     VMKRERYFPSAAASGLRTKTTREIGILSPFFIGDFFLKIFESLHRQLRDYDLILYNAQ
                     TPTRRREVIDRIVAEEKLCALIVA"
     gene            650..1864
                     /locus_tag="VFH83_10545"
     CDS             650..1864
                     /locus_tag="VFH83_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6486849.1"
                     /translation="MRRLVVVLVALGLVAGLAFAQTAQPPKSTVRIAFWTQEGESEGA
                     FQFAKKLADAFTAKYPNITFDLLNKETEALRQDFLAASLANSAPDLLLTVNDHAGPFT
                     VADVIQPVDKLFDMKQYVPSIVMGGKTWGVPVSSGNHLMLLYNKDLVKTPPKTTNDLI
                     AVGKTLTTGDRYAIVWNQSEPFWMVPWLGGFGGAVFAADGHTPTLNTPAMVNTLQFLY
                     DLKVKYAITPKEADYATADTLFKEGKAAMLINGDWSLGDYKKALGDKLGVAPLPQVSS
                     TGMWPAPYTSGKYFMIPKADSGDTLQAVVAFIQFYTSYDNQIDLVKSLTRLPGLRTAL
                     NSEAVKSDPILQGSALQMSLGTPMPSVVEMRANWDSMKPEMNAVLAGTKSPADAAKAM
                     QDAAVAAIKAMQ"
     gene            1976..3037
                     /locus_tag="VFH83_10550"
     CDS             1976..3037
                     /locus_tag="VFH83_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6486850.1"
                     /translation="MKSLTPQIMVSSLGQLGEVLLVILLAVAALELVLWLVLRRLLHY
                     RYALPLMLVAPGIVGLLLLNLYPIGFEIGLAFSNMNLLRFRNPTYSLSMAWQNLASVF
                     TAPVLKQQYFFAVFLRTALWTVIQVTFHVTLGLFLAILLNRPMRLRGLYRTLLVVPWA
                     LPQVVASLAWRGEFNYEYGFPNVMLTRLGLHAIQWKSDPFWNFVAMNVTNIWLGIPFM
                     VVILLGGLQSLSPSYFEAADMDGASGWRKLRSITLPLIQPILTPAVVLGIIWTFNNFN
                     VPYFINENNLETSDILVTALFRAAFDFNRYGFSAMFALVIFVILLAISIVYMRMVSFS
                     PATAVSRKARETLTVGENV"
     gene            3034..3906
                     /locus_tag="VFH83_10555"
     CDS             3034..3906
                     /locus_tag="VFH83_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6486851.1"
                     /translation="MTRRTFVRWFFYRARGDSPAKRSVIHLVLIIACILSVYPALRVV
                     SVSIRPGNRVLSTDLALIPQGATFDAYATVVRDRDFLLWIWNSLAITIATSLIGVIIA
                     STAAYGFSRWSFPGRTAGLIGLLTTQMIPAPMLMIPLYILAARLNLINTWRGLVVAYS
                     VSAIPFSIWILKGYYDTIPVELEQSAMIDGASRMGAYYRVILPLSAPSLAIAFLFNFT
                     SSWNEYLLARIILQKKEMLTWTLGLRQMQGQFQTQWAEFSAAALMISIPVMALFFYSS
                     KWLVSGLTLGSVKE"
     gene            3909..>5421
                     /locus_tag="VFH83_10560"
     CDS             3909..>5421
                     /locus_tag="VFH83_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HET6486852.1"
                     /translation="MRRRLLAVMGIAAALGVLAIVPAGAEEGGAEAAAARADDVLYVN
                     LIWHQHQPLYYQDPATGAYTRPWARVHATKDYYFMAATLARYPKVHATFNLTPVLLRQ
                     LDDLAAGAKDLYEVMAEKPADALTPDDKTFLVERFFDANRDHIIKRFPRYAELLAQKL
                     AAGTTDAAVRAFTASDFRDLQVWFNLAWFDPDSLGQPPLSDLVAKGRGFSEADKQILF
                     GQALRIVRQVVPIHRTLQDRGQIEVITTPYAHPILPLLYSTAIAARGDPSTPLPDWYS
                     FANDAVAQVRKSVDVYRAHYGREPLGMWPAEGAVAPEIVKMVAEAGYQWMASGEQVLA
                     RSLGMGDITRDSNDLVRQADALYRPYYVKSRDGARVAMVFRDQRLSDLIGFEYSGTPG
                     EDAASDLMRRLEAIRLALKAGPDRGPHLVSIILDGENAWENYDTNGREFLNALYRDLS
                     DSVSIRTTTVSDYLHQYPEQREIDTLWSGAWFCADYATWIGEPEENTAWNYLAK"
BASE COUNT          939 a         1809 c         1672 g         1001 t
ORIGIN      
        1 gccacgatga gggcgcagag cttctcctcc gccacgatgc ggtcgatcac ctctcgccgc
       61 cgcgtcgggg tctgggcgtt gtacaggatg aggtcgtaat cccgaagctg acggtggagg
      121 ctctcgaaga tcttcagaaa gaaatccccg atgaagaacg gcgagaggat cccgatctcc
      181 cgggtggtct tggtccgcag ccccgacgcg gcggcggacg ggaaatagcg ctcccgcttc
      241 atgacgccga tcacgcgctc ccgcgtggcg tgggagatcc cgtgtcggcc gttgaggacg
      301 cgggagaccg tggcggcggt cacccccgcc gcgctggcga tgtcatagat ggtcactcgc
      361 ttcttgcgcg cgcccatgga ccctcccgga aaaagcttga caacccgtcc ggtgttagta
      421 tacttcacgc aatcggttgc actatgttac ttcaggttgt cctcacgggc aagcgttccc
      481 ctcgttcttc cgggcgccgg gccttcccgg gtctcggccg ccagatcccg aacgaatccc
      541 tgcggttcat ccgcgccgcg gtcacacccg cccgcgccgc gaaggccgtc tcagactccg
      601 ccgccggtcc ccgggaccgc gccgcggaga cccagaatgg aggtgctgga tgagaagact
      661 cgttgtcgtg cttgtagcgc tcggcctggt ggccggcctg gcattcgctc agaccgccca
      721 gccgcccaaa tccacggtcc ggatagcttt ctggacccag gagggagaat ccgaaggggc
      781 gttccagttc gccaagaagc tggcggatgc cttcaccgcg aagtacccga acatcacctt
      841 cgatctcctc aacaaggaga cggaggccct ccgccaggat ttcctggccg ccagtctggc
      901 caattccgcg ccggacctgc tgctcaccgt caacgatcac gcgggcccct tcaccgtggc
      961 ggacgtcatc cagccagtgg acaagctctt cgacatgaag cagtacgtgc cgtccatcgt
     1021 catgggcggc aagacctggg gcgtgccggt ctccagcgga aaccacctca tgctcctgta
     1081 caacaaggac ctcgtgaaga cgcctcccaa gacgacgaat gacctgatcg ccgtgggaaa
     1141 gacactcacc accggggatc gctacgccat cgtctggaac cagtcggagc cgttctggat
     1201 ggtgccctgg ctgggcgggt tcggaggggc cgtattcgcc gcggacgggc acacccccac
     1261 gctgaatacg ccggccatgg tgaacaccct ccagttcctg tatgacttga aagtgaagta
     1321 cgccatcacc ccgaaagagg cggactacgc caccgcggat accctgttca aggaggggaa
     1381 ggcggcgatg ctcatcaacg gagactggtc cctgggagac tacaagaagg cgctcgggga
     1441 caagctgggc gtggcgccgc tcccccaggt gagctcgacc gggatgtggc ctgcgccgta
     1501 caccagcggc aagtacttca tgatccccaa ggcggactcc ggggacacgc tccaggccgt
     1561 ggtggcgttc atccagttct atacgagcta cgacaatcag atcgacctgg tgaagtccct
     1621 cacccggctg cccgggctgc gcacggcgct gaactccgag gcggtgaaga gtgaccccat
     1681 ccttcaagga tcggccctcc agatgtccct cggcacgccg atgccgtccg tggtggagat
     1741 gcgcgccaac tgggactcca tgaaaccgga gatgaacgcc gtgctggccg gcaccaagag
     1801 ccccgcggat gcggccaagg ccatgcagga cgcagcggtg gccgccatca aggccatgca
     1861 gtaggagcgg ctccacgaat ctattgcgga tcgggctccg gggcggtctc cccggggccc
     1921 gttttcgttg cgtggaacca gaaacggaac ggggcggtga gcaggaggtg atcgcatgaa
     1981 gtccctcacg ccacagatca tggtgtccag cctcggtcag ctcggcgagg tgcttctcgt
     2041 catccttctt gcggtcgccg cgctggagct tgtgctttgg ctcgtgttga ggcggcttct
     2101 gcactaccga tacgccctgc ccttgatgct ggtggcgccg ggcatcgtgg ggctgcttct
     2161 cctcaacttg tatcccatcg ggttcgagat cgggctcgcg ttcagcaaca tgaacctctt
     2221 gaggttccga aacccgacct actccctctc catggcctgg cagaacctgg cgagcgtctt
     2281 tacggctccg gtgctcaagc agcagtactt cttcgccgtg tttctgcgaa cggcgctctg
     2341 gaccgtcatc caggtcacct tccacgtgac tctgggtctc tttctggcca tcctgctgaa
     2401 ccgacccatg aggctgcgag ggctttaccg cacgctgctg gtggtgccct gggccctccc
     2461 ccaggtcgtg gcctccctgg cctggcgcgg ggagttcaac tacgagtatg gttttcccaa
     2521 cgtgatgctc acgcgcctgg gtctgcacgc catccaatgg aagtccgacc cgttctggaa
     2581 tttcgtggcg atgaacgtca ccaacatctg gctcggcatc cccttcatgg tggtcatcct
     2641 cctgggaggg ttgcagagtc tcagccccag ctacttcgag gccgccgaca tggacggggc
     2701 cagcggatgg cgcaagctgc ggtccatcac gcttccgctc attcagccca tcctcacgcc
     2761 cgccgtcgtg ctgggaatca tctggacctt caacaacttc aacgtcccgt acttcatcaa
     2821 cgagaacaac ctggagacct cggatatcct ggtgacggcc ctcttccgag cggccttcga
     2881 cttcaaccgc tacgggttct ccgccatgtt cgccctggtg atcttcgtga tcctgctggc
     2941 catctccatc gtctacatgc ggatggtctc tttcagcccg gccacggccg tatcacgcaa
     3001 ggctcgggag accctcacgg tgggagagaa cgtatgacgc ggcgaacctt cgttcggtgg
     3061 ttcttctacc gggcgcgggg agacagcccg gccaagcgat ccgtcatcca cctggtcctc
     3121 atcatcgcct gcatcctgtc ggtgtatccc gcgctgcggg tcgtgagcgt ctccatccga
     3181 cccggcaatc gggtgctgtc caccgacctg gccctgatcc cccagggggc gaccttcgac
     3241 gcctacgcga cggtggtgcg agatcgggac ttcctgctct ggatctggaa cagcctggcc
     3301 atcaccatcg ccacctccct gatcggcgtg atcatcgcct ccacggcggc ctatggattc
     3361 tcgcgctgga gcttccccgg gcgcacggcg ggtctgatcg ggcttctgac cacgcagatg
     3421 atcccggcgc ccatgctcat gattccgctc tacatcctgg cggcgcgact gaacctgatc
     3481 aacacctggc gtggactggt ggtggcctac agcgtgagcg ccattccctt cagcatctgg
     3541 atcctcaaag gctattacga caccatcccg gtcgagctgg agcaatccgc gatgatcgac
     3601 ggggcctccc gcatgggcgc ctactaccgg gtcatccttc ccctgtcggc gccgtctctg
     3661 gccatcgcgt tcctcttcaa cttcaccagc tcctggaacg agtacctcct ggcgcggatc
     3721 atcctccaga agaaggaaat gctcacctgg accttggggc ttcggcagat gcagggccag
     3781 ttccagaccc agtgggccga gttctccgcg gcagccctga tgatctccat tcccgtaatg
     3841 gcgctgttct tctactcctc caagtggctg gtctccggac tcacgctggg gtccgtaaag
     3901 gagtgaaggt gcgccggcgg ctcctggcgg tgatggggat cgcggccgct cttggggtcc
     3961 tggcgattgt acctgcgggg gcggaggagg gcggggcgga ggcggctgcg gcccgagcgg
     4021 acgacgtcct ctacgtgaac ctcatttggc accagcacca gccgctgtac taccaggacc
     4081 cggcaaccgg cgcatacacc cgcccctggg cgcgtgtaca cgccaccaag gactactatt
     4141 tcatggctgc caccctggcg aggtacccga aggtgcacgc gacgttcaac ctcaccccgg
     4201 tgcttctgcg ccagctcgac gacctggcag ccggggcaaa ggacctctac gaggtcatgg
     4261 cggagaagcc cgcggacgcg ctgaccccgg atgacaagac cttcctggtg gagcgcttct
     4321 tcgacgcgaa tcgggaccac atcatcaagc ggtttccgcg ctatgccgag ctcctggcgc
     4381 agaaactggc ggccggaacc acggacgccg ccgttcgcgc cttcaccgca tcggacttcc
     4441 gggacctcca ggtgtggttc aacctggctt ggttcgatcc ggattcgctg gggcagcctc
     4501 ccctgtcgga cctggtcgcc aaagggaggg gcttctccga agccgacaag cagatcctgt
     4561 tcggtcaggc gcttcggatc gtccgacagg tggtgccgat ccatcggacg ctccaggacc
     4621 gcggccagat cgaagtcatc accaccccgt acgcgcatcc catcctgccg ctgctctaca
     4681 gcacggcaat cgcggcgcgc ggggatccat cgacccctct gccggactgg tactcctttg
     4741 ccaacgacgc ggtagcccag gtgcggaaga gcgtcgacgt ttatcgggct cattacggtc
     4801 gagagcccct ggggatgtgg ccggcggaag gcgccgtggc gccggagatc gtcaagatgg
     4861 tggcggaagc cggttaccag tggatggcct ccggcgaaca ggtcctggcc cggtccctgg
     4921 ggatggggga tatcacccgg gattccaacg acctggtccg tcaggccgac gcgctctacc
     4981 ggccctacta cgtgaagagc cgggacggcg cgcgggtggc catggtgttt cgcgaccaac
     5041 ggctctccga cctgatcggg ttcgagtatt cgggcacgcc cggtgaggat gccgcctcgg
     5101 acctcatgcg tcgcctggaa gccatccggc tcgcgctcaa ggccggcccg gaccggggcc
     5161 cccacctggt gagcatcatc ctcgacggcg agaacgcgtg ggaaaactac gacaccaacg
     5221 gaagagagtt cctgaacgcg ctgtatcgcg acctgtccga tagcgtgagc atccggacca
     5281 cgacggtctc ggattacctc caccagtacc ccgagcaacg agagatcgac accctgtggt
     5341 ctggcgcctg gttctgcgcg gattacgcca cctggatcgg ggagccggag gagaacacgg
     5401 cgtggaacta cctggccaag g
//
LOCUS       DASRZS010000433         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2927011, whole genome shotgun sequence.
ACCESSION   DASRZS010000433 DASRZS010000000
VERSION     DASRZS010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2927011"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2328
                     /locus_tag="VFH83_10565"
     CDS             <1..>2328
                     /locus_tag="VFH83_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET6486853.1"
                     /translation="GTGRDGSLGAKAIKAELGMVIVQEPETARYDGMPRSAIAAGVAD
                     YVRAPQDMPVALIEYVRQSHGVAAATRREMIEKQASDMQKIFSIIRLRTRRDFSGYKV
                     STLRRRIERRMSVNHMETLGEYARFVADTPPETEALFKDLLINVTRFFRDPPAFDVLK
                     KLLVERLATRKDGETVRAWTAGCSTGEESYSLAILLQECIEQLGKYLDIQVFGTDIDA
                     DAIAVARAGIYPAAVTQDLNEDRVRRFFVRTDGQLQVRKELREKLVFAAQDITSDPPF
                     SRMDLISVRNVLIYLGAELQRRLIPVLHYALEGDGILFLGTAETIGEYADHFRVLDGK
                     WKIYGRQGSPDPHSTMPTPLSWNNLPEAQPPRTPEGLSGGVSERSLLQALGPSVLVDS
                     AFHIVYVHGETSRFLELAQGEPNMSIMDMARPGLKSEIATALIEAGTQRKPIVRERVR
                     NKRDGIVYATRIAVQPVVGVPERQGFAVVSFSEYPEISASPEPAVKGKGDTKIRKLEE
                     ELQHTKENLRSTIEELETANEELRSANEEYQSTNEELQSTNEEMETSREELQSVNEEL
                     LTVNNEHQNKIEELSIINDDMMNLLNSSNIATIFLDNALQIKRYTPDATRIFNLIGTD
                     VGRPLGHLTSNLHLENLESHARKVLETLVPFREVVQTRDGHWYSMRLHPYRTTRDAIA
                     GVVLSFVDIHEHVTEKLAVSRRLEIANEFALHLASDAQTPVLVLDAGLRVAMTNDAFR
                     RQLPVAAPEGQWIYEIQAGAWDTEEIHALLEVEMPR"
BASE COUNT          482 a          758 c          721 g          367 t
ORIGIN      
        1 cggcacgggc cgggacggat ccctgggcgc aaaagccatc aaggcggagc tgggcatggt
       61 gatcgtgcag gagccggaga ccgcccgcta cgacggcatg cctcgctccg cgatcgcggc
      121 gggagtcgcg gactacgtcc gcgcccccca ggacatgccg gtcgccctca tcgaatacgt
      181 gcgccagtcc catggcgtgg ccgcggccac acgacgcgag atgatcgaga agcaggccag
      241 cgacatgcag aagatcttct ccatcatccg gcttcggacc aggagggact tctcgggcta
      301 caaggtctca accttgaggc ggcggatcga gcgccggatg agcgtgaacc acatggagac
      361 gctgggagaa tacgcccggt ttgtcgcgga cacccccccg gaaaccgagg cattgttcaa
      421 ggaccttctg atcaacgtga cccggttctt tcgcgatcca cccgccttcg acgtgctgaa
      481 gaagctcctg gttgagcgcc tcgccacccg caaggacggc gagacggtcc gagcatggac
      541 ggcggggtgc tccaccggag aggagtccta ctccctggcc attctcctcc aggaatgcat
      601 cgagcagttg ggcaagtacc tggatatcca ggtgttcggc acggatatcg acgcggacgc
      661 cattgccgtt gccagggccg gcatctaccc tgccgctgtc acccaggatc tcaacgagga
      721 ccgggtccgg cgcttcttcg tgcgcaccga cggccagctc caggtaagaa aggagctgcg
      781 ggaaaagctc gtctttgccg cgcaggacat caccagcgat ccaccgtttt cccgcatgga
      841 cctgatctcg gtgcgcaacg ttctcatcta cctcggcgcc gagctgcagc gtcggctcat
      901 tccggtcctt cactacgccc tggagggaga cgggatcctc ttcctcggga ccgcggaaac
      961 catcggcgag tatgcggatc atttccgggt cctcgatggc aagtggaaga tctacggacg
     1021 gcagggatcc cccgacccgc attccacgat gcccacaccg ctgtcgtgga acaacctccc
     1081 cgaggcgcag cctcccagga cgccggaggg actgtcggga ggggtttccg agagatccct
     1141 tctccaggcg ctcgggccct ccgtgctcgt ggactccgcc tttcacattg tctatgtcca
     1201 cggagaaacc agccggttcc tcgagctggc gcagggcgag ccgaatatga gcatcatgga
     1261 catggcccga cccgggctga agagcgagat cgccaccgcg ttgatcgaag cgggcaccca
     1321 gagaaagccg atcgtccgcg agcgggtccg caacaagcgg gacggcatcg tttatgcaac
     1381 gcggatcgcg gtgcaacccg tggtcggcgt ccccgaacgc cagggctttg ccgtggtgag
     1441 cttctcggag tacccggaga tttcggccag cccggagccc gccgtcaaag ggaaaggcga
     1501 cacgaagatc cgaaagctgg aagaggagct gcagcatacg aaggagaacc tgcggagcac
     1561 catcgaagag ctggagaccg ccaacgagga gcttcgttcc gccaacgagg agtaccagtc
     1621 cacgaacgag gagctgcaaa gcacgaacga agagatggag acctcccggg aagagctcca
     1681 gtccgtcaac gaggagctcc tcaccgtgaa caacgagcac cagaacaaaa tcgaggagct
     1741 ttccatcatc aacgacgaca tgatgaacct tctcaattcc agcaacatcg cgaccatctt
     1801 cctggacaac gcgcttcaga tcaaacgcta cacgccggat gccacccgca tcttcaacct
     1861 catcggaacg gacgtgggca ggccgctggg tcacctgacg tccaacctcc atctggaaaa
     1921 cctcgagagc cacgccagga aggtattgga gacgctggtc ccgttccgcg aagtggtgca
     1981 gacccgagac ggccactggt actccatgcg cctgcatccc taccgcacga cccgggacgc
     2041 gatcgccggc gttgtgctgt cctttgtgga catccacgag cacgtgacgg aaaagctcgc
     2101 cgtgagccgc cggctggaga tcgccaacga gttcgccctc cacctggcct cggacgccca
     2161 gacgccggtc ctggtcctgg atgccggcct tcgcgtcgcc atgaccaacg acgccttccg
     2221 tcgacagcta ccggtggccg cgccggaggg tcagtggatc tacgagatcc aggcgggcgc
     2281 atgggacacg gaagagatcc acgcgctgct ggaggtggag atgccgcg
//
LOCUS       DASRZS010000434         6203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1770839, whole genome shotgun sequence.
ACCESSION   DASRZS010000434 DASRZS010000000
VERSION     DASRZS010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770839"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VFH83_10570"
     CDS             <1..1126
                     /locus_tag="VFH83_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6486854.1"
                     /translation="AFVAVAGVGLAEVLDQTPYPRLMICGALLLVFAAGLVINFGHFY
                     RPSRTLFTAIAVIQTASAIGVMVAGASPLFGAILFFVLNTVIAVGLPQPVALAWGAVP
                     VVALTACLLLRGERDWFSTVLTFSAGFFAFIAFALALRQAQTARAESQQLLEELTGTQ
                     GRLREMAVLEERQRLAREMHDAVGHRLTAASVLLEGAARLIRGDPDRATRMVETSREQ
                     VKEGLAEIRAAVSALREEPAGSRPLPEVLRALVDVFAQASPAEVTLAVDDGLEPDTER
                     KNVIVRTAQEALTNVQKHASASRVQLRLQMIDGAYVLSCRDNGRGPGSRADNGSSVGG
                     FGLPNLQERASSFGGSVELTTAPEGGALLRLVLPKGETHG"
     gene            1119..1808
                     /locus_tag="VFH83_10575"
     CDS             1119..1808
                     /locus_tag="VFH83_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6486855.1"
                     /translation="MGEGAGSGGRHATIRVLIVDDQKLMREGLRTLLELEEGIAVAGE
                     AADGAAAVDAYLRLHPDVVLMDLRMPGMDGVEAIRRIRGTDSEAKILVLTTFDEDALV
                     FESVRAGARGYVLKDLGGTQLAQAIRTIAAGGAALQPTVARKVMDAFARLAESTPGSE
                     PHLQEPLSARERAVLGLVARGHSNKEIAERLFLAEGTVKNHVSTILAKIGARDRTQAA
                     LQARQLGLIDE"
     gene            1860..2543
                     /locus_tag="VFH83_10580"
     CDS             1860..2543
                     /locus_tag="VFH83_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486856.1"
                     /translation="MNRKALLGRSLPLVLIWLFVARALVAGSAVSVGVRTGAAASLVL
                     SSYSQFVQTDLLGQGAGTVTPYPCFSFQVDAYGRVLLGSFLALETGLSVCQVGGGFMA
                     SGATEELVGISVTELAFPLVAVLLLRTGIGTFGLSLGAMAAIPLEAPVLVAKTNLAST
                     QTTLTPASSFTGLVAGVSLQREAGPGSLSFDLRYIHRLQSIVTKLPAEQGSVAPVTLE
                     ASVGYLFTF"
     gene            2554..4317
                     /locus_tag="VFH83_10585"
     CDS             2554..4317
                     /locus_tag="VFH83_10585"
                     /inference="COORDINATES: protein motif:HMM:NF021943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M30"
                     /protein_id="HET6486857.1"
                     /translation="MRSGSRARAGLVASAVLAILAGCSPFGGVLTKEGTAPDFLIQHV
                     PGEGTVPYSVDLGTGPHSVFFAFTNPSSQVSAPALPAVSGGNGSRSVRPLTPVTALAS
                     TVAPTPDRVMRFNQDPFRQGPFQRVGSRQIPTATHPRRDPTLPEGPDQVGEESNSWFD
                     VGADNTVYGVPSTCRYVSPQPVDTASGQRSLSIWVANDCWDGLKSRTVTQPMVDALAS
                     RFLTPGPSNDIYDWVCGMLGEEWGPHDGQDLIPPDQRITILLLDIQNDDSTQGGVIGY
                     YWAKDNFRDSSVYDPDPTSPSYFPVHSHQRIMFYVDAVMYANAEGGSWSPEGHWPCQV
                     FSALAHELQHMVNFYQKTVRHGLRRGCDPWINEMTSQCVEDLVADKLGTAGPRGVNPQ
                     DGSSGPGGNTSGRIPVFNADPMVSLSDWGAASSPASYAATYAFGAYLVRNYGGAALLR
                     AMVQSPQTDSTQILDAVRSVTGRSETLSSLLSKWGIAVLLSDRTDAPVGYQYNVGGFF
                     GSSVDGLSYRAGSMDFYKYQQAGSTGGEGPALYSGMGLVGERIPGPTSETLFEACKDS
                     GGAVSWTIDVPKGMELTVLVR"
     gene            4414..>6203
                     /locus_tag="VFH83_10590"
     CDS             4414..>6203
                     /locus_tag="VFH83_10590"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET6486858.1"
                     /translation="METNSKPFVTVNPAELPSHFDSPSVEKRLAEFWDRIGAYRYHAD
                     AAREDTFAVDTPPPTVSGSLHIGHVFSYTHTDIEVRYQRMRGRNIFYPMGWDDNGLPT
                     ERRVQNLFHVRCDPRLPYEPGLTLEPAGGDRGPLKVISRRNFIELCRTVTEKDELAFL
                     ELWRRVGLSVDWNEHYATIDEQCRRIAQLSFLDLYRKGHVYNREEPAMWDVDFQTAVA
                     QAEVEDRTVAGAYHRLTFGIEGEARTFTVATTRPELLPACVGVAAHPEDARYRDLLGR
                     SAVTPLFGAPVPIFASDRVDPLKGTGILMVCTFGDSSDVLWWRQQNLDLRQVIGRDGR
                     LTPVTFGDPGWPSANPSAANAAYAQLVGKRIGEARKLIVSLLREPASGPGGSQKPSLQ
                     GEPEPIQHPVNFYEKGEHPLEFIPTRQWFVRLLDKKDLLKEMGNRISWHPEHMRLRYL
                     NWTDNLQFDWCISRQRFFGVSFPLWYPLDADGNADFEHPILADPGQLPVDPMSATPPG
                     LEENARGKPGGFVGEPDVYDTWFTSSMTPQIGTRWPSDPERHKRLFPMDLRPQAHDII
                     RTWAFYTIAKSALHEGSIPWKNIALSGWIL"
BASE COUNT         1010 a         2069 c         2049 g         1075 t
ORIGIN      
        1 cgccttcgtg gcagtggcag gagtgggact ggccgaggtg ctggaccaga cgccctatcc
       61 tcgtctcatg atctgcggtg cccttctcct ggtcttcgcc gccggcctgg tcatcaactt
      121 cggccacttc taccggccat cgaggactct ctttaccgcg atcgccgtca tccagacggc
      181 atctgccatc ggcgtgatgg tggccggcgc gtccccgctc ttcggagcca tcctcttttt
      241 cgtcctgaac acggtgatcg ccgttggtct tccgcaaccg gtagcgctgg catggggcgc
      301 cgttcccgtt gtggcgctca cggcctgctt gctcctgcgc ggcgagcggg actggttctc
      361 cacggtgctc acgttctcgg ccgggttctt cgccttcatc gcctttgccc tggcgctgcg
      421 ccaggcccag accgctcggg cggaaagcca gcagctcctc gaggagctga ccggcaccca
      481 gggccgcctg agggagatgg cggtcctgga agagcgccag cgcctcgcca gagagatgca
      541 cgacgcggta ggccaccggc tcaccgccgc gagcgtcctc ctggaggggg cggcacggct
      601 gatccggggg gacccggacc gcgcgacccg catggtagag acatcgaggg agcaggtgaa
      661 ggaaggcctc gcggagatcc gggcagccgt gagcgcgctg cgggaggagc cggccggatc
      721 ccggccactc ccggaagtcc tgcgggcgct ggtcgatgtc ttcgctcagg cctctcccgc
      781 ggaggtgacc ctcgcggtcg acgacggcct tgagccggat accgagcgaa agaacgtgat
      841 cgtccgcacg gcgcaggaag cgctcaccaa cgtgcagaag cacgcatccg catcccgcgt
      901 ccagcttcgt ctgcagatga tcgatggagc ttacgtcctg agctgtcggg acaacggccg
      961 aggccctggc tcccgcgcgg acaacgggtc gagcgtggga gggttcggtc tcccgaacct
     1021 gcaggagcgg gcatcgagct tcgggggcag cgtggagctg acaaccgcgc cggagggcgg
     1081 cgcgctcctg cggctcgtgc tgccgaaggg tgaaacgcat gggtgaggga gccggctcgg
     1141 gcggccggca cgcgacgatc cgcgtgctta ttgtggacga tcagaagctc atgcgggagg
     1201 gccttcggac gctgctggag ctcgaggaag ggattgcggt tgcgggggag gcggcggatg
     1261 gggccgccgc ggtggacgcc taccttcggc tgcacccgga cgtggtgctg atggacctga
     1321 ggatgccggg catggacggc gttgaagcca tacggcggat ccgcggcacg gattccgagg
     1381 cgaagatcct cgtcctcacc acgttcgacg aggatgccct ggtgttcgag tccgtgcggg
     1441 cgggcgcccg agggtacgtg ttgaaggacc tgggaggcac ccagcttgcc caggccatcc
     1501 ggaccatcgc ggccggcggc gccgcgctgc agccgacggt tgcccgcaag gtgatggatg
     1561 ctttcgcccg gctcgcggag tccaccccgg gctccgagcc gcatctccag gagccgctct
     1621 cggcgcgaga acgagcggtc ctcggactcg ttgcgagggg ccacagcaac aaggagatcg
     1681 ccgaaaggct cttcctggcc gaaggcaccg tcaaaaatca cgtgagcacg atccttgcga
     1741 aaatcggcgc ccgcgaccgt actcaggcgg ccctccaggc ccggcaacta ggcctgatcg
     1801 acgaatagcg atttttcttg cgttcccggc cgggctccgg tagaatgacg ccccaaaagg
     1861 tgaatcgaaa agcgcttctc ggtcgatctc tgcccctggt gctcatctgg ctgttcgtcg
     1921 cccgtgcctt ggttgccggc tccgccgtct ccgtgggggt acgcaccggc gcggcggcga
     1981 gtctcgtcct ctccagctac tcgcagttcg tgcagacgga tctccttggc cagggcgcgg
     2041 gaacggtaac cccgtacccg tgcttctcct tccaggtgga cgcctacgga cgagtgcttc
     2101 tgggaagctt ccttgccctg gagaccggcc tgagcgtttg ccaggttggg ggtggattca
     2161 tggcgtcagg cgccacggaa gagctcgttg ggatctccgt gaccgagctt gccttcccgc
     2221 tcgtcgcggt cctgctcctc cggaccggga tcggcacctt cggtctcagc ctgggtgcca
     2281 tggcggcaat cccgctggag gctcccgtgc tggtcgcgaa gaccaacctg gccagcacgc
     2341 agacgaccct gacgcccgcc tcttctttca cgggactcgt cgcgggcgtg agcctccaga
     2401 gggaggcggg gcccggcagc ctctctttcg atctacgcta catacaccgc ctgcaatcga
     2461 tcgtcacgaa gctccccgcg gagcagggct ccgtcgctcc cgtcacgctg gaggcctctg
     2521 ttggctatct gttcaccttc tgacgttcgg caggtgcgct cgggctcgcg cgcccgggcc
     2581 gggctggtgg cctcagcggt tctggcaatc ctggccgggt gctcgccctt tggcggggtg
     2641 ctgacaaagg aaggaactgc tcccgatttc ctcatccaac atgtgcctgg cgaggggacc
     2701 gtcccctatt ccgtggatct gggcaccggt ccgcactcgg tcttcttcgc gttcaccaat
     2761 ccgagctccc aggtaagcgc cccggcgctt cccgcggtaa gcgggggcaa cgggtcccga
     2821 agcgtacggc ctttgacacc cgtcaccgcc cttgccagca ccgtggcccc tacgccggac
     2881 cgggtcatgc ggttcaacca ggatccgttc cggcaagggc ccttccaacg cgtcggttcg
     2941 cggcagattc cgacggcgac tcacccgcgg agggatccga ctctgccgga gggacccgac
     3001 caggtcgggg aggagagcaa cagttggttc gacgtggggg cggacaatac cgtgtacgga
     3061 gtgccctcca cctgccggta cgtctctccg cagcccgtgg ataccgcgag cggccagcgc
     3121 agcctgagca tctgggttgc gaacgattgc tgggacgggc tcaagtctcg caccgtcacc
     3181 cagcccatgg tggatgccct ggccagccgg tttctcaccc cggggccgtc gaacgacatc
     3241 tacgactggg tgtgtggcat gctcggagag gagtggggac cccacgatgg acaggacctg
     3301 atcccaccgg accagcggat cacgatcctc ctgctggaca tccagaatga cgattccacg
     3361 cagggagggg tgatcgggta ctactgggcc aaagacaact tccgggacag ctcggtgtac
     3421 gatccggatc ctacctcccc ctcctatttc ccggtgcact cccatcagcg gatcatgttc
     3481 tacgtggacg ccgtgatgta tgccaacgcg gagggcggat cctggagccc tgaaggccac
     3541 tggccatgcc aggtcttcag cgccctggcc cacgagctgc agcacatggt caacttctac
     3601 cagaagaccg tccgacacgg gctgcgccgc ggctgtgatc cctggatcaa cgagatgacg
     3661 tcccagtgcg tagaggatct ggtggcggac aaattgggga cggcgggacc tcggggcgtg
     3721 aacccgcagg acggcagctc ggggccgggg ggaaacacca gcggtcggat ccccgtgttc
     3781 aacgccgacc ctatggtctc cttgagcgat tggggagcgg ccagctctcc cgccagctac
     3841 gcggccacgt acgcctttgg cgcttacctg gtgcgcaact acggcggcgc ggccctgctt
     3901 cgcgccatgg tgcagagccc gcagacggat tcgacccaga tcctggatgc cgttcggtcc
     3961 gtgaccggcc gctccgagac tctgagcagc ctgctgtcca agtggggcat tgcggtgctg
     4021 ctttcggacc gtaccgatgc gccggtgggc taccagtaca acgtgggcgg gtttttcggc
     4081 tccagcgttg acggcctttc ctatcgcgcg gggtccatgg acttctacaa gtaccagcag
     4141 gccggatcca ccggagggga aggtcccgcc ctctattccg ggatgggtct cgtgggagag
     4201 cggatccccg gcccgacatc ggaaactctg ttcgaggcct gcaaggattc cggcggcgcg
     4261 gtctcctgga ccatcgacgt cccgaagggt atggaactga ccgtgctggt ccgataggcc
     4321 gcccggcggc tccgcgccgt ccttcgtggg cccccgacgc ccgcggctgg ctcttgcggc
     4381 tgcccgcctt caatcgtaga ctctttccca cgaatggaaa caaactcgaa acccttcgtc
     4441 acggtgaacc ctgccgagct tcccagtcat ttcgactctc cgagcgttga gaagcggctg
     4501 gcggagttct gggaccggat cggcgcgtat cgctaccacg cggacgccgc ccgggaggat
     4561 acgtttgcgg tggatacccc gccgccgacg gtctcggggt ccctgcacat cggtcacgtg
     4621 ttcagctaca cccatacgga catcgaggtt cgctaccagc ggatgcgcgg gcgaaacatc
     4681 ttctacccga tgggctggga cgacaacggt ctgcccacgg aacggcgcgt gcagaacctc
     4741 ttccacgtgc gctgtgatcc ccggctgccc tacgagcccg ggctgaccct ggagccggcc
     4801 ggtggagacc gagggcctct gaaggtcatt tcccgaagaa acttcatcga gctttgcaga
     4861 acggtcaccg agaaggacga gctggcgttc ctcgagttgt ggcggagagt cgggctctcg
     4921 gtggactgga acgagcatta cgcgaccatt gacgagcagt gtcgccgtat cgcccagctc
     4981 agcttcctgg acctgtaccg aaagggccac gtctacaacc gggaagagcc ggccatgtgg
     5041 gacgtggact tccagactgc ggttgcccag gcggaggtag aggaccgcac ggtcgccggc
     5101 gcataccacc gcctcacctt cgggatcgaa ggggaagcgc gcacctttac cgtggcaacc
     5161 acgcgacccg agctcctgcc ggcctgtgtc ggtgtcgcgg cgcatcccga ggacgcgcgg
     5221 taccgggacc tgctgggtcg cagcgcggtc acccccctct tcggcgctcc ggtgccgatc
     5281 ttcgccagcg atcgcgtgga tcccctgaag ggcaccggta tcctcatggt ctgcaccttc
     5341 ggagactcca gtgacgtcct gtggtggcgc cagcagaatc tggacttgag acaggtgatc
     5401 ggacgagacg gcaggcttac gcccgtcacg ttcggggacc ccggctggcc cagcgcaaac
     5461 ccctccgccg ccaacgcagc gtatgcccag ctcgtcggca agcggatcgg cgaagcgcgc
     5521 aagctcatcg tctcgctgct gcgagagcca gcatcggggc ccggcggcag ccagaagcca
     5581 tccctccagg gcgagcccga gcccattcag catccggtca acttctacga gaagggcgag
     5641 caccccttgg agttcatccc cacgcgccag tggttcgtgc gcctcctgga caagaaggat
     5701 ctcctgaagg agatggggaa ccgcatttcg tggcatcccg agcacatgag gctccgttac
     5761 ctgaactgga ccgacaacct gcagttcgac tggtgcatca gccgacagcg gttcttcgga
     5821 gtgtcgttcc cgctctggta cccgctggac gcggacggga acgccgactt cgagcacccc
     5881 atcctggcag acccgggcca gctccccgtg gatcccatga gcgcgacgcc tccggggctc
     5941 gaagagaacg cccgcggaaa gccgggcgga ttcgtcggag agcccgatgt ctacgacacg
     6001 tggttcacga gctccatgac gccgcagatc gggacccggt ggccctcgga ccccgaacgg
     6061 cacaagcggc tcttcccgat ggacctgcgg ccccaggctc acgacatcat ccggacctgg
     6121 gcgttttaca ccatcgccaa gtcggccctg cacgaggggt cgatcccgtg gaagaatatc
     6181 gccctctccg gctggatcct gga
//
LOCUS       DASRZS010000435         3783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3695596, whole genome shotgun sequence.
ACCESSION   DASRZS010000435 DASRZS010000000
VERSION     DASRZS010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3695596"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFH83_10595"
     CDS             complement(<1..466)
                     /locus_tag="VFH83_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6486859.1"
                     /translation="MSCGTALVTGATNGIGWEIAFLLAEHGYHLLVTGRDPARLRKLE
                     TDLRGKVPVKGVAADLSREEGVATLIAAVRDSGAVPEILVNNAGLGDRGPFVEADLER
                     QRAIMEVNMVSLVRLTRELLPSMVQRGHGRILNVASTAAFLPGPLMSIYYASK"
     gene            complement(469..1815)
                     /locus_tag="VFH83_10600"
     CDS             complement(469..1815)
                     /locus_tag="VFH83_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486860.1"
                     /translation="MRMVSVRALGRWWGGVEALLVATTMLSLASCQVRGAQTGQSPIL
                     KFLQRASGLIAYIAPDGNVNIIDQKGGRKRALTADAGANGSGEGVLYAFPTWSPDRSL
                     LAYVRLTGSGQGSPVECSLVVAAPDGSTQTRLFTGTRLQPFYLFWAPDSRKISVLSSV
                     QGEQQLELGVATVRPDPGYSALDRGSPYYWDWLGGGASIISHVNIGMAGSAEERLSLV
                     SLSQQSARTDLPFTAGAFQAPAVSPDGKTIAYVVGDPQKFEIHLRSVDGASDRTVASG
                     SGAVYLSYAPDGKRIAYLSAESTAPVPQGTLHVLDLPDAKVDRAVAESPVISFFWAPN
                     SRLIAFVEPGDPGNLDTQFQQGQRQIVLKIAGCDPQTGKTWTIAQFPTSRGFLNLLPF
                     FDQYQRSATLWSPDSRHLVFTALSAEGGPGLFIASADGNLKPVRLVAGDSAFWSWR"
     gene            complement(1891..2937)
                     /locus_tag="VFH83_10605"
     CDS             complement(1891..2937)
                     /locus_tag="VFH83_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET6486861.1"
                     /translation="MSEGALLTLSAPRYRVLVGTGGIGSGSFFQVNGNHTLGREESRS
                     GRYLHRRDYCKLHIVCHYVKRLAGDALTVIPVGRVGNDEVGRRLLSEMQEAGLRTNRV
                     RVLEGAPTLFSFCIQYPDGSGGNLTTDNSACSAVTAEMIDDAAEDLPSPGGGIVLAVP
                     EVPFAPRRRLLRLGGERGLLRVASFTSEELPRAEAEGVLADVDLLAMNGDEARSLLPG
                     SGLGPTEDVVARFAGYAGQRYPALRWVITAGAEGSWSWDGRTTSRRLALPVRVVGASG
                     AGDAHLAGIIAGLAGGLELHPAHDLGCILAAASLASEHTINTEITREQLRELARLADL
                     HDEALLRMIGSTSA"
     gene            complement(2937..>3783)
                     /locus_tag="VFH83_10610"
     CDS             complement(2937..>3783)
                     /locus_tag="VFH83_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486862.1"
                     /translation="PYDELATHPDRRVPFRLVRDSDEMGGLMAEELVSAIQAVTGRGE
                     TFRVIIPCGPSCWYKPFTDLVNRRRVSLQQVQVFHMDECLDWQAQELPRDHPYSFRGF
                     MERHFYDPIDTGLAVPERNRFWPRLETIEDIRRRIREAPIHLTYGGWGQDGHIAYNQA
                     RRHPYSPISLEELRGSTIRLQENNWDTIIALAQRTFGGAWQLVPPMSVTLGLSECLSA
                     HRVRLFSDTGAWKQTALRVALFSDPTPEYPITLLQEHPDALLTATVETARHPVSLHPE
                     WDLGL"
BASE COUNT          659 a         1290 c         1273 g          561 t
ORIGIN      
        1 ccttggaggc gtagtagatc gacatcaagg gccccggcag gaacgccgcg gtcgatgcca
       61 cgttcaggat ccgcccgtgc ccgcgctgga ccatggacgg caggagctcc cgggtcagcc
      121 gcaccaggga gaccatgttc acctccatga tggcgcgctg acgctcgaga tccgcctcca
      181 cgaaggggcc gcgatccccc agtccggcgt tgttcacgag gatctccggt actgcgccgg
      241 agtctcgaac cgccgcgatg agcgtagcca cgccctcctc ccgcgagagg tccgcggcca
      301 cacccttcac cgggaccttg ccgcgaaggt ccgtctccag cttccgcagt cgagccgggt
      361 cccgtccggt gaccaggagg tggtaaccat gctccgccag gaggaaggca atctcccatc
      421 caatgccgtt ggtcgcgccg gtcacgaggg ccgtaccgca agacatcgtc acctccagga
      481 ccagaatgcc gaatccccgg caaccagccg caccggcttg aggttgccat cggcgctggc
      541 aatgaagagc cccggtcccc cctccgcgct cagcgccgtg aagaccaggt gccggctgtc
      601 cggcgaccag agggtcgccg atcgctggta ctggtcgaag aacggcagca ggttcagaaa
      661 cccgcggctg gtcgggaact gggcgatggt ccacgtcttg ccggtctggg gatcgcagcc
      721 ggcaatcttc aggacgatct gccgctgccc ctgctggaac tgggtatcca ggtttccggg
      781 gtccccgggc tcgacgaagg caatcaaacg gctgttcggc gcccagaaga acgagatgac
      841 cgggctttcg gccaccgcgc ggtcgacctt ggcatccggc aggtcgagga catgcagcgt
      901 gccctggggg accggagccg tggactcggc ggagagatac gctatgcgct tcccgtccgg
      961 agcgtaggag aggtagacgg ctcccgagcc ggacgccacg gtccgatccg aggccccatc
     1021 cacggaccga aggtggatct cgaacttctg cgggtccccc acgacgtacg cgatggtctt
     1081 gccgtcgggg gacaccgcgg gcgcctggaa ggctccggcg gtgaacggaa ggtcggtccg
     1141 agcggactgc tggctcaaag agaccagcga gagccgttcc tcggcggagc cggccatccc
     1201 gatgttcacg tgggagatga tcgaggcgcc gcctcccaac caatcccagt agtacggtga
     1261 accgcggtcc agagcgctgt agccgggatc gggacggacg gtggccacgc ccagctcgag
     1321 ctgctgttcc ccctgcacgg acgagagcac gctgatcttg cgcgagtccg gcgcccagaa
     1381 gaggtagaag ggctgaagcc gcgtgccggt gaaaagcctg gtctgggtcg acccgtccgg
     1441 ggccgcgacc accagggagc actccaccgg tgacccctgg cccgagcccg tcagccgcac
     1501 gtacgccagc agggaccggt ccggcgacca ggtggggaac gcgtacagga ctccttctcc
     1561 cgagccgttg gccccggcgt ccgcggtgag cgccctcttc cgtcccccct tctggtcgat
     1621 gatattcacg ttgccgtcag gagcaatgta cgcgatcagg ccggatgcgc gctgcaggaa
     1681 cttgaggatc gggctctggc cggtctgagc tcccctgacc tgacaggacg ccagagagag
     1741 catggtcgtg gcgaccagca gcgcctcaac ccctccccac caacgcccca acgcccgaac
     1801 cgaaaccatc cgcacggcgc tcgcgctacc cccctacgct ccggaaagga tacgggaagg
     1861 gatgggggaa ggcaaaccga accaggctcc tcaggcggaa gtagagccga tcattcgcag
     1921 aagcgcctca tcgtggaggt ccgccagccg ggccagctcc cggagctgct cccgggtgat
     1981 ctcggtgttg atcgtgtgct cggaggccag ggaggctgcc gccaggatgc agcccaggtc
     2041 gtgcgccggg tgcagctcca gtccgcccgc cagcccggcg atgatcccgg cgaggtgcgc
     2101 atctccggcc ccgctcgcgc cgacgacgcg aacgggaagg gcaaggcggc gtgacgtcgt
     2161 cctcccgtcc catgaccagc tcccctcggc gccggcggtg atcacccagc gcagcgcggg
     2221 gtaccgctgc cccgcgtatc ccgcgaaacg ggccacgaca tcctccgtgg gccccagacc
     2281 gctgcccggc aagagagagc gggcctcgtc cccgttcatc gccaggagat ccacatcggc
     2341 aaggacgcct tccgcctctg cgcgaggcaa ctcttcactg gtgaagctcg caacccggag
     2401 aaggccgcgt tcgccgccca gccgcaggag ccgccgtctg ggggcaaacg gcacctcggg
     2461 gacggcgagc acgataccgc cgcccggaga ggggagatcc tccgcggcat cgtcgatcat
     2521 ctccgccgtc acggcggagc aggcggagtt gtccgtggta aggtttccac ccgacccatc
     2581 cggatactgg atgcagaacg agaagagggt gggagccccc tccaggaccc ggacccggtt
     2641 cgtccggagg ccggcctcct gcatctcgga aaggagccgt cgtccgacct cgtcgttgcc
     2701 gacccggcct acggggatga ccgtgagcgc gtccccggca agcctcttca cgtaatggca
     2761 gacgatgtgg agcttgcagt agtcgcgccg atgcaggtat ctcccactgc gactctcttc
     2821 ccggcccagg gtgtggttgc cgttcacctg gaagaagctg ccggacccga tgccgcccgt
     2881 ccccaccagg acgcgatagc gcggagcgga gagcgtgagg agcgcgccct cgctcactac
     2941 agcccgaggt cccactcggg gtgcaggctc acggggtgac gggccgtctc gacggtcgcc
     3001 gtcagcaagg catccgggtg ctcttgaagg agggtgatgg ggtactccgg cgtgggatcg
     3061 ctgaacagag cgacccgcag ggccgtctgc ttccacgcgc cggtatccga gaagagcctg
     3121 acccggtggg cgctcaggca ctccgacagc cccagggtaa cggacatcgg cggcacgagc
     3181 tgccaggctc cgccgaaggt gcgctgggca agggcgatga tggtgtccca gttgttctcc
     3241 tggagccgga tcgtcgagcc gcgaagctcc tccaggctga tcgggctgta gggatggcgc
     3301 cgcgcctggt tgtaggcgat gtgcccgtcc tgaccccatc cgccgtacgt caggtggatc
     3361 ggagcctcgc ggatgcggcg tcggatgtct tctattgttt ccagccgagg ccagaaccgg
     3421 ttgcgctcgg gcacggcaag cccggtatcg atgggatcgt agaagtgccg ctccatgaac
     3481 ccgcggaagc tgtagggatg atcgcgagga agctcctggg cctgccagtc gagacactcg
     3541 tccatgtgga acacctggac ctgctggagg gagacccggc gccgattgac caggtccgta
     3601 aacggcttgt accagcagct aggcccgcac gggatgatga cgcggaaggt ctccccacga
     3661 ccggtcaccg cctggatcgc ggagacaagc tcctccgcca tgagaccgcc catttcgtcg
     3721 gagtcccgca ccagccgaaa aggaaccctg cggtccggat gcgtggccag ctcgtcatac
     3781 ggg
//
LOCUS       DASRZS010000436         8717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2928866, whole genome shotgun sequence.
ACCESSION   DASRZS010000436 DASRZS010000000
VERSION     DASRZS010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2928866"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFH83_10615"
     CDS             <1..280
                     /locus_tag="VFH83_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6486863.1"
                     /translation="EGTWKVLDGLAQLAKDKGCTMSALALAWNMAQPGVTSPILGPNS
                     VAQLDDNLGSLSVKLSEEDMKRVDALIPPGTHTEDYYRAEFGPHPQRI"
     gene            complement(317..1375)
                     /locus_tag="VFH83_10620"
     CDS             complement(317..1375)
                     /locus_tag="VFH83_10620"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6486864.1"
                     /translation="MNARERMLAAIHHERCDRVPTDIWATPESWAKLERAFGSGFDRV
                     MDALHIDGMETIAPEYAGPPIAAVGPDEQVDFWGMRSRRVGHATGAYWEQSFFPLAFA
                     QSIEDLDRYRWPSADWFDYSGLREKAAAAREKRVVQSGYMAPFYYHNLLRGLERSLMD
                     PLDDAELTREILKRICDFFFEYHLRVFEACGGMVDVAQVTDDYGSQTGPLIGLPTFRD
                     FYKPHLQRFIDLCHAFGVTVFHHDDGAIRPFLPELVTMGIDILNPVQHACPGMEMEGL
                     KRDFGHRLCFHGGIDNQKTLPFGTPKEVREEVRHAIDALSPDGTGYILAPCHNIQAVS
                     PLENVVAMYDEAFRYGCY"
     gene            complement(1372..2133)
                     /locus_tag="VFH83_10625"
     CDS             complement(1372..2133)
                     /locus_tag="VFH83_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HET6486865.1"
                     /translation="MALAPASQPVVHTESIVVRVFETDANGALAPRSLCDFLQEAADR
                     HVALLGVTVEKLKERSLTWILSRLRLSIQRLPTKGETLTIRTWHAGIDRLFSLRDYQV
                     TDAAGSPVASAITAWLAFDLAARKPVRVQSVFDGGETSPLPRSLDVKLDRLPGAGQPF
                     DERSISVRWSDLDINRHVNNSRYAEWVVEGVGSELRDHASLQGLDLDFLAETQAGGSV
                     AVRTSRGDTGVLEHTVARREDGIEVARGRTLWRNP"
     gene            2261..4258
                     /locus_tag="VFH83_10630"
     CDS             2261..4258
                     /locus_tag="VFH83_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486866.1"
                     /translation="MLPLDLLKQLRAYAVRTRKGTFDLRQFQTTQLKGLTPGEDIETP
                     IATLAAEGSIRIQGATAEGRQVTIAGFAVLALSEEYRQIAMDPERVFPSDRAVSAAVA
                     AEAMDVVDVKVELGALIDAAKPETPGIVKIQFPEDVEPLIVPRSVVSTLLIDACVAKI
                     GQHLRERENGAYIEAKLVGFLKGQEVLLHSAIQDVSARTEKAAATIGTPSDFNFRFWT
                     QLCNFLLADIRAKKEKTERDQGLCQAAYIIGYAVFHTKGSAQRDQERASDYRSLEGRL
                     RKPPFLFGYQELYQLTDEKGTAYASKHGREFIHGFLRDKAVRKEQETIPFLLRLHLSA
                     QNKDYFIQRDLLVPVFLKKLGEASEALRRQYLDEWTAALRQDHPPDVSRSDGAFRRDV
                     EKRIKAGFPLLAALANGPVLYIAAEEGHLPEESVAELRRCFAVENILKPADQLLELSR
                     QELLKQVRTYLPVWQTMPILGALVRFFRRLGSGRDGEAASAAQGREAPQLVDHPVGAG
                     APRGGGAAGAEGVGGSGSAAVGVGAVGGHAGARPQQVSATRRAALIQHQSQVRSLMAQ
                     RVPENKTVDEVLRELAEKWNPLYEARRKQNLVEDVNALVRDFLRPIRRSLLSRPPDPK
                     RIRALATQLCESQSLAGIKKRDILQQYVELYMLRCLQVKTL"
     gene            complement(4285..5031)
                     /locus_tag="VFH83_10635"
     CDS             complement(4285..5031)
                     /locus_tag="VFH83_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6486867.1"
                     /translation="MKHVSLGMLSVLMPAGGGHGLPEDTFPPDPLALKITFISHATLM
                     MRYGDLKGSAQPIVIHVDPVGEYANYARMEKADLILVTHEHADHLDPVAIQALRKPST
                     TVVASEKCLPKIPGAVPLRNGQSTEAHGIRMTAVPAYNIRHLRSPGQPFHPKGDGNGY
                     LVKVGSRQVYVAGDTENTPEMKALKGVDIAFLPMNLPYTMTPEMVADAALAMRPKVLY
                     PYHFGDTDTARLVSLLKSATDIEVRIRPMK"
     gene            5101..6297
                     /locus_tag="VFH83_10640"
     CDS             5101..6297
                     /locus_tag="VFH83_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET6486868.1"
                     /translation="MLAIRDVRTILTAPAGIDLVVVRVDTSEPGLYGLGCATFTQRAL
                     AVRTAVDEYLRPFLIGKDATRIEDLWQTAMVSAYWRNGPVLNNAISGVDMALWDIAGK
                     RAKMPLYQLLGGKCREAAAVYRHADGRDEREVEQNLRAFLSQGYRFVRCQMGGYGGKQ
                     SLPPAPRGAVPGAYYDPDAYSRSVPKLFDHLRSAVGSEVELLHDIHERLAPIEAVRLA
                     KTLEKHRLFFLEDPLAPEHLEWFRAIRAQCATPIAMGELFVHPNEWRPLVAERLIDFI
                     RVHLSAIGGITPAIKLAHLCGEFGVRTAWHGPGDVSPIGHAANVHLDVASPNFGVQEW
                     SGFSDALREVFPGCPEEKAGYAYPPETPGIGVDLDEKRAAAYPPIGGLPAWTLARTPD
                     GSSARP"
     gene            complement(6332..7309)
                     /locus_tag="VFH83_10645"
     CDS             complement(6332..7309)
                     /locus_tag="VFH83_10645"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HET6486869.1"
                     /translation="MNKRFGFRLTGGSFFPIFVAFYIPAVALTVLASAWSYGAAFRGA
                     TSSPWPSLGAAAVTILLYLVFAVPFLRILLPAVILDGIPFSFRGSVGRFVGMNLLGLL
                     LSIVTIGIYAPWYIARVTSYVAGEAGWKDKRIEFTGKGGRLFVILLLTVVLPAVVIAV
                     ALAIALYTVLTAGGASPVGSATMTQLMTYVLIVLLFPSYAYELYRWYLTNVRIGDYGI
                     RWNTRFWRSVGFIYLQILLTVVTLSVYWPAAWIRGYRYFANRSDITRQEGVCASVRFR
                     GTPGGGFGLLWGQTLLSIVTLGIYIPWAVAKIGNWVASNTTLEAPEGGG"
     gene            complement(7345..7746)
                     /locus_tag="VFH83_10650"
     CDS             complement(7345..7746)
                     /locus_tag="VFH83_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486870.1"
                     /translation="MARAIDQENSFLQVLLKLIPSEIIAVYLFIQGIIPRLPLAHLLA
                     ALVMVGITPVYLRWATKVRSRAHLAVSTVSVVVWIYAMGIGPLRFVPPPYYEPWHGAV
                     LLAVWTLVPPMFLTGSQEPAPTRARRARGRL"
     gene            complement(7798..8592)
                     /locus_tag="VFH83_10655"
     CDS             complement(7798..8592)
                     /locus_tag="VFH83_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HET6486871.1"
                     /translation="MTVLERLSQSFVVRLVLTLIVYGFYACVLGASLAPSTALVIWAC
                     LRFLSPAVLSGAVPALGLLVLFCLFLGAAVFVFFTFGLLLMGTVIRLLSLGVKPGRHD
                     AASLPVLLWMVLNGVFTLAWRIILPLVPMTPVSMMFHRLSGCRIGKNVWINTHHLIDS
                     YMISIGDNTLIGGEAVLSPHVYEGGKLLIQRITIGKNCLIGGHSYISPGVTVGDGSVI
                     GMRAYVRKGKQIPPGTRITSVAGLPFERALDLERGTFTRVRSALGA"
BASE COUNT         1629 a         2778 c         2875 g         1435 t
ORIGIN      
        1 cgaagggaca tggaaggtcc ttgacggtct ggcccagctc gccaaggaca agggctgcac
       61 catgagcgcg ctcgcccttg cctggaacat ggcgcagcca ggcgtcacga gccccatcct
      121 agggccgaac agcgtggccc agctcgatga caaccttggc tccctgtcgg tcaagctgag
      181 cgaggaggac atgaagcgcg tggatgcgct gatcccgccg ggtacccaca cggaggatta
      241 ctaccgggcg gagttcggtc cccatccgca gcggatctag tcgcgccgtc cgcgtgccca
      301 ggagcccgct ccggcgctag tagcagccgt accggaaggc ctcgtcgtac atcgccacga
      361 cattctcgag cggactgacg gcctggatgt tgtgacaggg agccaggatg tagccggtgc
      421 catccgggga gagggcatcg atggcgtggc gaacctcctc cctgacctcc ttgggcgtgc
      481 cgaacggcag cgtcttctga ttgtcgatgc cgccgtgaaa gcagagccga tggccgaaat
      541 cgcgcttgag gccctccatc tccattccag ggcaggcgtg ttggaccgga ttgaggatgt
      601 cgatgcccat ggtgaccagc tcgggaagga atggtcggat cgctccgtcg tcatggtgga
      661 agacggtcac cccgaacgcg tggcacaggt cgataaagcg ctgcaggtga ggcttgtaga
      721 agtcccggaa ggtgggcagg ccgatcagag gaccggtctg gctgccatag tcgtccgtga
      781 cctgcgccac gtccaccatg ccgccgcagg cttcgaagac cctcaggtgg tactcgaaga
      841 agaagtcgca gatccgcttc aggatctccc gagtgagctc ggcgtcatcg agcgggtcca
      901 tgagggagcg ctccagaccc cgcaggaggt tgtggtagta gaacggcgcc atgtacccgc
      961 tctggacaac ccgcttttcc cgggccgccg cggctttttc gcggagcccc gagtagtcga
     1021 accagtccgc gctcggccac cggtagcggt ccaggtcctc gatcgattgg gcaaaggcaa
     1081 gagggaagaa ggactgctcc caataggcgc ccgtggcatg acccaccctg cggctacgca
     1141 tgccccagaa gtccacctgc tcatcggggc cgaccgccgc gatcggcgga ccggcgtact
     1201 ccggcgcgat ggtctccatg ccgtcgatgt ggagggcgtc catcacccgg tcgaagccgc
     1261 tgccaaaggc gcgctcgagc ttggcccatg actccggcgt tgcccagatg tccgtgggga
     1321 cccggtcaca ccgttcgtga tggatcgcgg cgagcattcg ttcccgtgcg ttcatgggtt
     1381 cctccacagc gttcggcctc tggccacctc gatgccgtcc tcccgacggg caacggtgtg
     1441 ctcgagcaca cccgtatcgc ctcggctcgt tcggaccgcg acactgccgc ctgcctgggt
     1501 ctcggccagg aaatccagat ccagaccctg gaggctcgca tggtcccgca gctcggaccc
     1561 cacgccctcc acgacccatt ccgcgtagcg gctgttgttc acgtggcggt tgatgtccag
     1621 atcgctccac cgaacgctga tgcttctctc gtcgaacggc tgcccggcgc ccggcaggcg
     1681 gtccagcttc acgtccagcg accgcggcag gggactcgtc tctccgccgt cgaacacact
     1741 ctggactcgg accggtttac gggcggcgag gtcgaacgcc agccaggccg tgatggccga
     1801 ggccaccggc gagccggctg cgtccgtaac ctggtagtcc cgcagcgaga acaggcggtc
     1861 gatgccggcg tgccaggtgc ggatggtgag cgtctcaccc ttggtgggga gccgctggat
     1921 gctcaaccgc aagcgcgaga ggatccacgt gagagaacgc tccttcagct tctccaccgt
     1981 gaccccgagc agcgccacgt gtcggtccgc ggcttcctgg aggaagtcac agaggctccg
     2041 cggtgcaagg gcgccgttgg catcggtctc aaacacgcgc acgacgatgg actcggtgtg
     2101 caccacgggc tgggaagccg gcgcgagggc catcggttct cctttcgagg gaaggtgcgg
     2161 catcagggta tcatagacgg gcggttgcgg catccacgaa ccacggatgc tcgaacgctt
     2221 gtcagcacgc tcgggatgaa ctaaactttt ccaatagccc atgcttccgc tcgaccttct
     2281 gaaacagctt cgagcctatg cagtgcgcac gcgaaagggc acgttcgacc tgcgtcagtt
     2341 ccagacgacg cagctcaagg gactgacccc gggggaggac atcgagacgc cgatcgcgac
     2401 gctggccgcg gaagggtcca tccggatcca aggcgcaacc gcggagggtc gccaggtgac
     2461 gatcgccggc ttcgcggtac tggcgttgag cgaggagtac cgccagatcg cgatggatcc
     2521 cgagcgggtg ttccccagtg atcgcgcggt gagcgccgcc gttgccgcgg aagccatgga
     2581 cgtcgtggac gtgaaggtgg agctgggcgc gctcatcgac gccgcaaagc ccgaaacgcc
     2641 tgggatcgtc aagatccagt tccccgagga tgtggagcct ctgatcgtcc cccggtccgt
     2701 ggtttccacc ctgttgatcg acgcctgcgt ggccaagatc gggcagcatc tgcgggagcg
     2761 ggagaacggc gcctacatcg aggcaaagct cgtaggtttc ctgaaggggc aggaggtgct
     2821 cctccactcc gccatccagg acgtgagcgc ccgcacggag aaggcggccg cgaccatcgg
     2881 cacgcccagc gacttcaact tccggttctg gacccagctc tgcaacttcc ttctcgcgga
     2941 catccgagcc aagaaagaga agaccgagag agaccagggt ctctgccagg ccgcctacat
     3001 catcgggtac gcggtgttcc acaccaaggg cagcgctcaa cgcgatcagg agcgcgcgtc
     3061 ggactaccgc tctctggagg ggcggctgcg caagcccccg ttcctgtttg gctaccagga
     3121 gctgtaccag ctcaccgacg aaaagggcac cgcctacgcc tccaagcacg ggcgggagtt
     3181 catccacggc ttcctgcggg acaaggcggt ccgaaaggag caggagacca tcccgttcct
     3241 gctgcgtctg cacctctcgg cccagaacaa ggactatttc attcagcggg atctcctggt
     3301 cccggttttc ctcaagaagc tgggggaagc ctccgaggcg cttcgccggc agtatctgga
     3361 cgagtggacg gcggcgctcc gccaggatca tccaccggat gtctcgcgca gcgatggggc
     3421 gttccgtaga gacgtcgaga agcggatcaa ggccggcttc ccgctgctcg ccgctcttgc
     3481 caacggaccc gtactgtaca tcgccgcgga agaggggcat ctccccgagg agagcgtggc
     3541 agagctgcga cggtgttttg ccgtggagaa catcctcaag cccgcggatc aactcctgga
     3601 gctctctcgc caggagctgc tcaagcaggt tcgcacctat cttcccgtct ggcagacgat
     3661 gccaatcctc ggggcgctgg tgcggttctt ccggcggctt gggagcggaa gggatgggga
     3721 agcagcgagc gcggcgcagg gacgggaggc tccgcagcta gtcgatcatc ccgtgggggc
     3781 cggggctccg cgcggcggtg gcgccgcggg cgcggagggt gtcggaggct cagggagcgc
     3841 tgcggtcggg gtgggggctg tcggagggca cgccggggcc cggccacaac aggtctccgc
     3901 gacacgaagg gctgcgctga tccagcatca gagccaggtg aggtccttga tggcgcagcg
     3961 ggtcccggag aacaagaccg tggacgaggt cctgcgcgag ctggccgaga agtggaaccc
     4021 gctgtacgag gctcgacgca agcagaacct ggtcgaggac gtcaacgccc tggtccggga
     4081 ctttctacgt cccatccggc ggagcctcct gagccggccg ccggacccca agcgcatacg
     4141 ggccctcgct acccagctct gtgagagtca gagcctggcg ggcatcaaga agcgggacat
     4201 cctccagcag tacgtcgagc tgtacatgct gcgctgcctg caggtaaaga cgctgtagga
     4261 gatcgggccc aggccaggga ttgcctactt catcgggcgg atgcgcacct caatatccgt
     4321 ggcgctcttg aggagcgata cgagcctggc cgtgtccgtg tccccgaagt ggtagggata
     4381 gagcaccttc ggccgcatgg ccagcgcagc gtccgccacc atctcgggag tcatggtgta
     4441 cggcaggttc atcggaagga acgcgatgtc cacgcccttc agggccttca tctccggtgt
     4501 attctccgta tcccccgcca catagacctg cctcgacccg acctttacca ggtatccgtt
     4561 gccgtctccc tttggatgga agggttggcc cggcgatcgc agatgtcgga tgttgtacgc
     4621 cggtacggcc gtcatccgaa tcccgtgggc ctctgttgat tggccgttgc gcaggggcac
     4681 ggcacccgga atcttgggca ggcacttctc gcttgccacg acggtggtgg agggcttgcg
     4741 gagggcctgg atggcgaccg ggtccaggtg gtccgcgtgc tcgtgggtaa ccaggatcag
     4801 gtccgctttc tccatccgcg cgtagttcgc gtattcgccg accggatcca cgtggatgac
     4861 gatcggctgt gcggagccct tcaggtctcc atagcgcatc atcagcgtgg catgagagat
     4921 gaaggtgatc ttcagcgcca ggggatccgg cggaaacgtg tcctcaggga ggccatgtcc
     4981 cccgcccgca ggcatcagaa ccgagagcat gccgagggaa acgtgtttca tggagcgcct
     5041 ctccatcgag tatacatcga gcgggcgtcc agtctaccct gagcctccgg aggctccagg
     5101 atgttggcaa tccgtgacgt tcggaccatt cttaccgcgc cggccggcat cgacctcgtg
     5161 gtggtgcgtg tggatacctc cgagccgggc ctctacggtc tggggtgcgc cacgttcacc
     5221 cagcgcgccc tggcggtaag gacggcggtg gacgaatact tgcggccgtt cctcatcggc
     5281 aaggacgcga cgcggatcga ggatctctgg caaacggcga tggtgagcgc ctattggcgg
     5341 aacggacccg tcctgaacaa cgccatctcc ggcgtggata tggctctctg ggatatcgcc
     5401 ggcaagcgcg cgaagatgcc tctgtaccag ctcctgggcg gcaaatgtcg ggaggcagcc
     5461 gcggtgtacc gccacgcgga cggtcgcgac gagcgcgagg tggagcagaa cctccgtgcc
     5521 ttcctttcgc agggctaccg gtttgtccga tgtcagatgg gtgggtatgg agggaagcaa
     5581 tccctgccgc cggccccgcg gggggcggtg cccggcgcct actatgaccc ggacgcctat
     5641 tcccgcagcg tcccgaagct gttcgatcac ctccgttctg ccgtcggttc tgaggtcgag
     5701 ctgctgcacg acattcacga acggctggcc cccatcgagg ccgtgcgcct tgccaagacc
     5761 ctggagaagc accgactctt tttcctggag gaccctctgg cgccggagca cctggaatgg
     5821 ttccgcgcga tccgcgccca gtgcgcgacg cccatcgcca tgggggaact cttcgttcac
     5881 cccaacgagt ggcgacccct ggtggccgag cgcctgatcg atttcatccg ggtgcacctg
     5941 agtgcgatcg gcggtatcac cccggcgatc aagctggccc acctctgtgg cgagtttggc
     6001 gttcgcaccg cgtggcatgg ccctggcgac gtctctccca tcgggcacgc ggcgaacgtg
     6061 cacctggacg tggcctcccc gaacttcggt gtgcaggagt ggagcgggtt cagcgatgct
     6121 cttcgggagg tattccccgg gtgtcccgag gagaaagccg gctacgccta cccgccggag
     6181 accccgggta tcggagtgga cctggacgaa aagagagctg ccgcttatcc gccgataggc
     6241 gggctcccgg cctggacgtt agcccgaacc ccggatggca gctcggcccg accgtgagcg
     6301 tttctggggc tccgggccgg cggctgacgg atcagccgcc accctccgga gcctccagcg
     6361 tggtgttgct ggccacccag ttcccgattt tcgcgacagc ccacggaatg taaatcccca
     6421 gagtaacgat cgagagcagg gtctgccccc acagaagccc gaacccgccg ccaggcgttc
     6481 ccctgaaccg gaccgacgcg caaacgcctt cctgacgagt aatgtcgctc ctgttggcga
     6541 aataccgata tcctcggatc caggccgcgg gccagtacac ggagagggtc acgaccgtca
     6601 gaaggatctg caggtaaatg aagccgaccg atcgccagaa ccgggtgttc caacggattc
     6661 cgtagtcccc gatccgcacg ttggtgagat accagcggta cagctcgtag gcgtaggaag
     6721 ggaagaggag gacgatgagc acatacgtca tgagctgggt catggtcgcg cttcccacgg
     6781 gggaagctcc gccagccgtc agcacggtgt agagcgcgat ggccagggct acggcgatca
     6841 cgacggccgg caggacgacg gtgagcagga ggatgacgaa aagcctgcct cccttgccgg
     6901 tgaactcgat gcgcttgtcc ttccagccgg cctcccccgc cacgtagctc gtgacccgcg
     6961 cgatgtacca gggcgcatag atcccgatcg tgacgatgga gaggagcaaa ccaaggaggt
     7021 tcatgccgac aaaccggcct accgaaccgc gaaatgagaa cggaatccca tccaggatca
     7081 ccgcgggaag caggatccgc agaaagggta ccgcgaacac caggtacaga aggatggtga
     7141 ctgccgcggc ccccagggac ggccatgggg aagacgtggc gccccggaac gcggccccgt
     7201 agctccacgc gctggccagg acggtgagag cgaccgcggg gatgtagaac gcaacgaaga
     7261 tcggaaagaa tgacccgccg gtgagccgaa agccgaaccg cttgttcatg atcatccccc
     7321 tcgtttaccg atgatacccg aacatcatag acggccacgc gcgcggcggg ctcgggttgg
     7381 tgccggctcc tgcgagcccg tcagaaacat cggaggcacg agggtccaga ccgcgagcag
     7441 taccgccccg tgccagggct catagtaggg cggcggcacg aaccgcagcg gtccgatccc
     7501 catggcgtag atccagacca ccacggaaac ggtggagacc gcaaggtgag cgcgggagcg
     7561 caccttcgtt gcccaccgaa ggtagacggg ggtgatgccg accatcacca gagccgcaag
     7621 caggtgcgcc aggggcagac gtgggatgat cccctgaatg aagagataga ccgcgatgat
     7681 ctcactcggg atcagcttga gaagcacctg caggaaggag ttctcctgat cgatcgccct
     7741 cgccatacat tttcctttcg agccctgccc gttccgtcat tccctcggct gtctccccta
     7801 cgcacccagc gccgatcgga ccctggtgaa agtgccgcgt tccagatcga gcgcccgttc
     7861 gaacggcaga ccggcaacgc tcgtgatccg cgtgcccggg gggatctgct tgcccttgcg
     7921 cacataagcg cgcatgccga tgacgctccc gtcccccacg gtgactccgg ggctgatgta
     7981 ggaatgcccg ccgatcaggc agttcttccc gatggtgatg cgttggatca ggagcttgcc
     8041 gccctcatag acgtggggag aaagcaccgc ctcgccaccg atcaacgtgt tgtctccgat
     8101 gctgatcatg taggagtcga tgaggtggtg cgtgttgatc cacacgttct tgccgatccg
     8161 gcagcccgat agtcggtgaa acatcatgga gaccggggtc atcggcacca ggggcaggat
     8221 gatccgccat gccagcgtga acacgccgtt cagcaccatc cagaggagca cgggcaggga
     8281 agcggcatcg tgacggcccg gcttcacccc gagggagagg agacggatga ccgtccccat
     8341 cagcaagaga ccgaatgtga aaaaaacgaa cacggccgcg ccgaggaaga ggcagaagag
     8401 caccaggaga cccaacgccg gaactgctcc ggaaagaacc gcgggcgaaa gaaaccgcag
     8461 gcaagcccag atcaccagcg ctgtggatgg cgccaggctg gcgcccagaa cgcaggcata
     8521 gaagccgtag acgatcagcg tcaggaccag cctgaccaca aaggattggc tgaggcgctc
     8581 caataccgtc atacccacta ttgtagaccg cccgggtggg gcgtcaatgg cacggtcggc
     8641 gtccctaagc gggggccagc ccatgccgct ggacggcagg ccggttagcc ttctaccaga
     8701 gctaccgtga ggtcgtt
//
LOCUS       DASRZS010000437         8106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1009584, whole genome shotgun sequence.
ACCESSION   DASRZS010000437 DASRZS010000000
VERSION     DASRZS010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009584"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..965
                     /locus_tag="VFH83_10660"
     CDS             183..965
                     /locus_tag="VFH83_10660"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET6486872.1"
                     /translation="MEKQKIFWVVLAVSILVVIVLITGVFLLRQRPAGAIASSPGTIN
                     PISEPGTQIYEYQREVPSAAQQSGSGQQVQHFYIGEGAPGSGGAASGAASGTASGTTA
                     QGTGSPAGASAGSAPGSATPPSTGQPGVTGAGGNPAVSATVTPAANPPARPKQVATPK
                     PGGPKPSVKPALTQRVLEYWIQTGSYKSQSKAEELSGTLSDKGLTARVFSYASKGDTY
                     YRVRIGPYGSSGEAQKFLVIVKGVQGLESSFVSQVHASRTLN"
     gene            complement(921..2162)
                     /gene="bshA"
                     /locus_tag="VFH83_10665"
     CDS             complement(921..2162)
                     /gene="bshA"
                     /locus_tag="VFH83_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018976427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HET6486873.1"
                     /translation="MDGNRASADGEHAGPATEEHFRIGICCYPTAGGSGVVATELGKH
                     LAERGHSVAFVSYANPLRLGELPPRVCYHEVDPDSHPLMQQFPHSLTLTAKMVEVART
                     HRLQILHVHYAIPFAAAAIIARQIAPELGLKVITTLHGTDITLVGSTPSLKPVTQWSI
                     EQSDAVTAVSRYLQEETYRQLPVRQRIDVVHNFIDPDRHGARVPSCIPRKASERQVTL
                     MHISNFRPIKRVDDVVRIFERVHRQMESRLVLVGDGPEYGRVRELVDSLGLSDSVRYV
                     GVVDEVASLLAAADVLLLPSSTESFGLVALEAMASGVPVVASAVGGLPEVVEHGVTGF
                     LAPVGDVEAMADYCLKLLQDCGESRQYARAARERATRLFDYRYIVPMYEAVYRRALGL
                     PAPEDASSVQGPARVDLRNKA"
     gene            complement(2162..3079)
                     /gene="epsC"
                     /locus_tag="VFH83_10670"
     CDS             complement(2162..3079)
                     /gene="epsC"
                     /locus_tag="VFH83_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HET6486874.1"
                     /translation="MSQDNGQPSPDFVDSIVERILDSYRELGGINHLEGPNLPSRQSV
                     ERIVESLQSIVFPGFRAEENLDLEGLRYATGQRVVEVSRALTVEVEKSLRYTCRTRGD
                     CKWRGDCRTEAEHIVREILGGIPDIRRRLNLDVHAADRGDPAARSQEEVILAYPGVEA
                     ILVHRIAHELWVREVAMLPRIMSELIHGKTGIDIHPGAQIGDSFFIDHGTGVVIGETT
                     IIGNNVKLYQGVTLGALSVQKELANRKRHPTIEDDVTIYAGATILGGDTTVGHGSIIG
                     GNVWLTESVPPYSRLYHNPTQSRARRSVG"
     gene            3143..3610
                     /locus_tag="VFH83_10675"
     CDS             3143..3610
                     /locus_tag="VFH83_10675"
                     /inference="COORDINATES: protein motif:HMM:NF014711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HET6486875.1"
                     /translation="MGSSAPLGVSWLPLSVSLVSAVVVQVACQAFKVVFYSIRERRPA
                     FSRFISAGGMPSAHAAFVTSLSVSVGLWNGFRSEVFAVACVFSVIIAYDAWRLRGAVQ
                     DQARALQRLLAIHPEVKVAALNENLGHSLPEIGAGLVVGGGASALIYLLLRGL"
     gene            complement(3634..4737)
                     /locus_tag="VFH83_10680"
     CDS             complement(3634..4737)
                     /locus_tag="VFH83_10680"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET6486876.1"
                     /translation="MKQERMPMTQVRESYQVSWYRCPIPTDRLRALTVRSDLRGAVQT
                     LGYLALVVALGLVSWFFYSRSLWGWMVVALFCYGTVFSFTPGLVTHELSHGTVFKTKW
                     LNSFFLRFYSLVSWTHFHQYRRSHTFHHLYTLHPKGDREVVLPATPSLHPLRLFLLFT
                     VSVQNFWNALRWTAALAFLGKFWNEWSEDVFADDAAARRQARGWAWVLLGFHTAVIAV
                     SIVFHLWLLPVLITVGPFIASWWVYFVGMPMHTGLRDNVPDFRLCVRTITLDPFSRFI
                     YWYMNYHTEHHMYAAVPCYNLPRLADAIASDMPRPRSVLEAWVEMRRIYKKQKTEPTY
                     QFDTPLPPTAHRANLPQDSLAASIGDLAPRGLA"
     gene            complement(4803..5384)
                     /locus_tag="VFH83_10685"
     CDS             complement(4803..5384)
                     /locus_tag="VFH83_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET6486877.1"
                     /translation="MEFAELVSRCRSYRRFDGQHAISAEILRALVSLARCAPSAANRQ
                     PLRFVLSCEASTNARIFETLAWAAYLKEWPGPEPKERPTGYIVILLDRTISQGADTDV
                     GIVAQTMLLGATALGLGGCMFGAIRREELAARLGLPERFSIPLVLAIGKPVERVVLEG
                     LPADGSIRYYRDAEATHHVPKRSVEELIQAVYS"
     gene            complement(5416..6015)
                     /locus_tag="VFH83_10690"
     CDS             complement(5416..6015)
                     /locus_tag="VFH83_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486878.1"
                     /translation="MRRVRSALLCGILGATLSCAQPPQAIIEAARTAVNAAAKNADIV
                     TYAPESLRAAQDDLAAMDAEADRQARRFALSRDFKALETLAANASDAAQLAQSNALES
                     KARVEQDAATLADQVGGAIHDFEARLWSARRLRGVQQSVIDGLSDLGNDSRASLDDAR
                     GDLASGSYASAKAKLSAILDRLDAAQQTIDEQTGIARAR"
     gene            6281..7636
                     /locus_tag="VFH83_10695"
     CDS             6281..7636
                     /locus_tag="VFH83_10695"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET6486879.1"
                     /translation="MRSITRWAAPLILLAAGTSLFAQGLGIKRVWNLIIQVDVPGATI
                     LVDNKPIPGNATRVPESIHNVKVQAPGYAPFSIDVNVNRDTTLPVHLQSVSFPLSLST
                     NVPGAQVWLDGILMTGPPVAAWGTHTVKVSAPGYQDYNASFTVNGPTALQVTLVPMGI
                     LLTVNANVGGATVLVNNVAKGGLPYTEYLPPGTYAVRVQAPGYSDFMTSVALNQPITV
                     NAMLQPQLFPVTLNLNVQSPIVMIDGVRMPVTPNVSMGQHTLQISAQGYMDYTGSFTV
                     TGPTVLQIALNPQALQLSVNANVDGAQVMVNNVSKGPAPYAESLPIGTYAVRVFADGY
                     LDYQANVSLTQPVALNVQLQPKPVVPATMAVVIPRPFVDSDIRPGDPQSTIRIWVDDK
                     LVNPKRELAGIQIPPGKHRVRIGSGALMVQLGDYVFQPGINYTLELGLTAVVKTPTAQ
                     Q"
     gene            7814..>8106
                     /locus_tag="VFH83_10700"
     CDS             7814..>8106
                     /locus_tag="VFH83_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486880.1"
                     /translation="MRRALIVLLILSPLLALYAQSPSPAAQGDALNDKGQYLDARKIE
                     LDAAAAASPAEKAELYWRAARETMELGDLSDKARKPQADTLAIFSEGEGYADKA"
BASE COUNT         1366 a         2733 c         2686 g         1321 t
ORIGIN      
        1 gggctgcgcc aggcgctgac ccgggcccgc gctcaacggg acatcccccg cggggcgtcc
       61 gtcgcccccc ctcaatccgg cgccgtcgct gtcgatactt atactgtacc gaaccggggg
      121 agcgcgcgct ccctcgatcg acgcgtcgag cgcctggttc ggcgcctgag aggctagaag
      181 gaatggagaa acagaagatc ttctgggtcg tgctggccgt gagcatcctc gtggtgatcg
      241 tgctgatcac cggtgtcttc ctgcttcgcc agaggccggc cggcgccatc gcatcctctc
      301 ccggcaccat caaccccatc tcggagccag gcacccagat ctacgagtac cagcgtgagg
      361 tcccctccgc ggcccagcag tccggcagcg gtcagcaggt ccagcacttc tacatcgggg
      421 agggagcgcc cggttctggg ggcgccgcct ccggcgccgc ttccggtacc gcctccggta
      481 ccacggcgca agggacgggg agccctgccg gtgcgtccgc cggctcagcg ccaggctccg
      541 ctaccccgcc gtcaacggga cagccgggcg taacgggggc aggtggcaat cccgctgtct
      601 cggcgaccgt gactccggcg gccaacccgc cggctcggcc caagcaggtc gccaccccga
      661 agcccggcgg gcccaagccg tcggtgaaac cggccctgac ccagcgggtc ctggagtact
      721 ggatccagac cggctcctac aagagccaga gcaaggccga ggagctttcc ggcacgctgt
      781 cggacaaggg tctgacggca cgggtgttct cctacgcgtc caagggagac acgtactacc
      841 gggtgcggat cggaccgtac ggcagctcgg gggaggctca gaagttcctg gttatcgtga
      901 agggcgtgca gggtctggaa tcaagctttg tttcgcaggt ccacgcgagc aggaccctga
      961 actgaggaag catcttccgg cgccggcagc cccagggctc gccgataaac ggcctcgtac
     1021 ataggcacga tgtaccggta gtcgaaaagc cgggtggcgc gctcccgcgc cgcccgggca
     1081 tactgccgac tctctccaca atcctgtaga agcttcaggc aatagtccgc catggcctcg
     1141 acgtccccga cgggagccag gaagcccgta accccgtgct ccacgacctc cggcaatcca
     1201 cccacggcgc tcgcgaccac cggcacgccg ctggccattg cctccagggc caccaggccg
     1261 aagctttccg tcgagctggg cagcaggagc acgtccgccg cggccaggag gctggcaacc
     1321 tcgtccacca cgccgacgta ccggaccgaa tcggagaggc ccagcgagtc gaccagctcc
     1381 cgaaccctgc catactccgg cccgtctccc accagcacga ggcggctctc catctggcgg
     1441 tggacccgct cgaagatgcg gaccacgtcg tccacgcgct tgatggggcg gaagttggag
     1501 atgtgcatca gggtgacctg gcgctccgac gctttgcgcg ggatgcagct cggcacccga
     1561 gcgccgtggc gatccggatc gatgaaattg tggacgacgt cgatcctctg gcggacgggg
     1621 agctggcggt aggtctcttc ctgcagatag cgcgacacgg cggttaccgc atccgactgc
     1681 tcgatggacc actgggtcac gggcttgagg gacggagtgc tccccacgag cgtgatgtcc
     1741 gtgccgtgga gcgtggtgat caccttcagc cccagctccg gagcgatctg gcgcgcgatg
     1801 atggccgcgg ccgcaaacgg gatggcgtaa tggacgtgca ggatctgcag gcgatgggtc
     1861 cgcgccacct ctaccatctt ggccgtgagc gtgaggctgt gcgggaactg ctgcatcagg
     1921 gggtggctgt ccgggtccac ctcgtggtag cagacccggg gcgggagctc tcccagccgc
     1981 agcgggttgg cgtagctcac gaacgcaacc gagtggccgc gctccgccag gtgcttgccc
     2041 agctcggtgg caacgacgcc gctgcccccg gcggtagggt agcagcagat cccgatccgg
     2101 aagtgctctt cggtggcggg gccggcgtgc tccccgtccg ccgacgctcg gttgccgtcc
     2161 atcaacctac gctccgtctg gcgcgggact gcgtcgggtt gtggtacagg cgggagtagg
     2221 gcggcacgct ctcggtcagc cacacgttcc cgccgatgat cgagccgtgg cccacggtgg
     2281 tgtctccgcc caggatcgtc gcgccggcgt agatcgtcac gtcgtcctcg atcgtgggat
     2341 gacgcttgcg gttggccagc tccttctgca cgctgagggc ccccagggtg actccctggt
     2401 agagcttgac gttgttgccg atgatcgtgg tctccccgat caccaccccc gttccatggt
     2461 cgatgaagaa cgagtctccg atttgagcgc cggggtggat gtcgatcccg gttttcccgt
     2521 ggatcagctc gctcatgatc cgcgggagca tggccacctc ccggacccag agctcatggg
     2581 cgatgcggtg caccaggatg gcctccacgc cggggtaggc caggatgacc tcttcctggg
     2641 aacgggccgc cgggtctccg cggtccgcgg cgtggacgtc caggttgagc cggcgtcgga
     2701 tatccgggat tccccccagg atctcccgca cgatgtgctc cgcctcggtc cgacagtccc
     2761 cgcgccactt gcagtccccg cgggtcctgc aggtgtatct caagctcttc tcgacttcta
     2821 cggtcaaggc gcgggatacc tcgaccacgc gctgcccggt ggcgtagcgc aggccttcca
     2881 ggtcgaggtt ctcctccgcc cggaaccccg ggaagacgat ggactgcagg gactccacga
     2941 tgcgctctac gctctgtcgg gacgggaggt tgggtccctc caggtggttg atcccgccga
     3001 gctcgcggta cgaatccagg attcgttcca cgatggaatc cacgaaatcc ggtgatggct
     3061 ggccgttgtc ctggctcatc gtgaaaaagg tgatccgccc ggcggcgttt gtcaaggcga
     3121 gcggattgtc gtactatgcc ccatgggctc gtcggcgccc cttggggtct cctggctgcc
     3181 tctcagcgtc agcctggtga gcgcggtcgt ggtgcaggtg gcctgccagg ccttcaaggt
     3241 cgtgttctac tcgatccgcg agagacgccc cgccttttcg cgcttcatct ccgccggcgg
     3301 catgccgagc gcccacgcgg cgttcgtcac gtctctctcc gtgtcggtcg ggctctggaa
     3361 cggcttccga tccgaggtat tcgccgtggc ctgcgtcttt tccgtgatca tcgcctatga
     3421 cgcgtggagg ctccgcggag ccgtccagga ccaggcgcgg gccttacagc ggctcctggc
     3481 gatccatccg gaagtgaagg tcgccgccct caacgagaac ctggggcact ccctgccgga
     3541 gatcggtgcc gggctcgtgg tcggcggtgg agcctcggca ctgatctatc tcctgctgcg
     3601 cggactatag gagggtccgg cgcagccggg cccctacgcc aggccccgcg gcgccaggtc
     3661 tccgatggat gccgccaggg agtcctgagg caggttcgcg cgatgggccg tgggcggcag
     3721 cggcgtatcg aactggtagg tgggctccgt cttctgcttc ttgtagatcc gacgcatctc
     3781 cacccaggcc tccagcacgg atcgtggccg cggcatgtcc gacgcgatgg catccgccag
     3841 acggggcagg ttgtagcacg gcacggcggc gtacatgtga tgctccgtgt ggtagttcat
     3901 gtaccagtag atgaaccggg aaaacgggtc cagcgtgatc gtgcggacgc agagccggaa
     3961 gtccgggacg ttgtcccgca gcccggtgtg catgggcatg cctacgaagt acacccacca
     4021 actggcgatg aacggaccga cggtgatgag cacgggcagc agccagaggt ggaacacgat
     4081 cgacacggcg atgacggccg tgtggaaccc caggaggacc catgcccacc ctcgggcctg
     4141 gcggcgcgcg gccgcgtcat ccgcgaagac gtcctcggac cactcgttcc aaaacttgcc
     4201 caggaatgcc agggccgccg tccatcgcag cgcgttccag aagttctgca cgctcacggt
     4261 gaagagcagg aacaggcgca gcggatgcag ggacggggtg gcgggcagga ccacttcgcg
     4321 gtcacccttg gggtgaagcg tgtagaggtg atggaaggtg tggctgcgcc gatactggtg
     4381 gaagtgggtc cagctcacca ggctgtagaa ccgaaggaaa aacgagttca gccatttcgt
     4441 cttgaagacc gtgccgtggg aaagctcgtg ggtgaccaga cccggggtga aggaaaagac
     4501 ggtgccgtaa cagaacagcg ccaccaccat ccagccccac aggctccgac tatagaagaa
     4561 ccaggacacc aggcccaacg ccaccaccag ggcaaggtac cccagggtct gcacggcacc
     4621 ccggagatcg cttcggacgg tcaacgcccg cagccgatcg gtcgggatgg ggcagcggta
     4681 ccagctcacc tgataactct cgcgcacctg ggtcatcggc attcgctctt gcttcatggg
     4741 gaaagtatac tgaacgaccg cggggcaggg gccggcggcc gctgtccgcc gaagcgccgg
     4801 gctcagctgt agacagcctg gatcagctcc tccacggagc gcttggggac gtgatgggtg
     4861 gcctccgcgt cccggtagta gcggatggag ccgtccgccg gcaggccctc cagcaccacc
     4921 cgttccaccg gcttgccgat ggcgagcacc agagggatgg agaaacgctc cggcaggccc
     4981 agccgcgcgg cgagctcctc ccggcggatc gcgccgaaca tgcacccgcc caaccccagc
     5041 gcggtagccc ccagaagcat ggtctgggcg acaatcccca cgtccgtgtc ggcgccttgg
     5101 gagatggtgc gatccagcag gatgacgatg tagcccgtgg gtcgctcctt cggctcgggc
     5161 ccgggccatt ccttgaggta cgcggcccag gcgagggttt cgaagatccg cgcgttggtg
     5221 gacgcctcac aggagagcac gaagcgcagg ggttgccggt tcgccgcgga gggggcgcat
     5281 cgcgccaggg acaccagggc gcgcaggatc tcggcgctga tggcatgctg gccgtcgaac
     5341 ctccgatagc ttcggcaccg ggaaaccagc tcggcaaact ccattgcgcc ctcctgccgc
     5401 gcgccgtgcc cgggcttacc gagcgcgcgc gatgcccgtc tgctcgtcga tggtctgctg
     5461 cgcggcgtcc agccggtcca ggatagcgga cagcttggcc ttggcggagg cgtaggatcc
     5521 agaggccagg tcgccgcgcg catcgtccag gctggcccgc gaatcgttgc ccaggtcgga
     5581 gaggccgtcg atcacgctct gctgcacccc gcgcagccgg cgggccgacc acaaccgggc
     5641 ctcgaaatcg tggatggcgc cgcccacctg gtccgcgagc gtggcagcgt cctgctcgac
     5701 cctcgccttg gactccaggg cgttggactg ggccagttgc gccgcgtcac tggcgttggc
     5761 ggccagcgtc tcgagggcct tgaagtcccg ggacaacgcg aaacgccgcg cctggcggtc
     5821 tgcttcggcg tccatggcgg caagatcgtc ctgcgccgcg cgaagggact cgggagcata
     5881 ggtcacgata tcggcattct tggccgcggc gttcaccgcg gtccgcgcag cttcaatgat
     5941 ggcttgagga ggctgggcgc aggacagggt tgccccgagg atcccacaga gcaaggcgga
     6001 gcgcacacgt ctcatctcag agagtcccct tcgaacaagg gccgggctcg gcccggtctg
     6061 cgcgacccgg tcgagcgcga cccggagttt gctgcaagcc gcgaatatcg gctcggtccg
     6121 cctcaggcat ggtaccagcc cgccgtttcc cgatccggat gacaggaatc ccgggtagac
     6181 aacccgattt ccaggcgtac gtcaagagga tcgtccaaaa ctttttgaaa aaccgctatc
     6241 cgggtgtata ctgaaatcgc tcaaaggaag gaggcacccg atgagatcca tcacgcggtg
     6301 ggcggcaccg ttgatcctct tggcggccgg cacatcgctc ttcgcgcagg ggctcggaat
     6361 caagcgagtc tggaacctca tcatccaggt cgacgtcccc ggtgcgacca tcctggtcga
     6421 caacaagccg atcccgggga acgccacgcg ggtccccgag tccatccaca acgtcaaggt
     6481 gcaggcgccc ggctatgccc cgttcagcat cgacgtgaac gtcaaccggg acacgaccct
     6541 gccggtccac ctccagtcgg tctccttccc gctgtccctg tccacgaacg tcccgggcgc
     6601 gcaggtctgg ttggacggca tcctcatgac cggcccgccc gtcgctgcct gggggaccca
     6661 caccgtcaaa gtgagcgctc cgggctacca ggactacaac gcctccttca cggtcaacgg
     6721 ccccacggcg ctccaggtga cgctcgtacc catggggatc ctgctcaccg tgaacgccaa
     6781 cgtcggcggg gccaccgttc tggtgaacaa cgtggccaag ggtggcctcc cgtacaccga
     6841 gtaccttccg cccggcacgt acgccgtgcg ggtccaggcg cccggctact ccgacttcat
     6901 gacctcggtc gcgctgaacc agcccatcac ggtcaacgcg atgctccagc cgcagctctt
     6961 tccggtgacg ctcaacctca acgtgcagtc gccgatcgtg atgatcgatg gcgttcggat
     7021 gcccgtgacg cccaacgtga gcatgggtca gcacacgctc cagatcagcg cgcagggcta
     7081 catggactac accggcagct ttaccgtgac cggtcccacc gtcctgcaga tcgcgctgaa
     7141 tccccaggcc ctgcagctct ccgtgaacgc gaacgtggac ggcgcccagg tcatggtcaa
     7201 caacgtctcc aagggcccgg ctccgtacgc cgagagtctg cccatcggga cctacgcggt
     7261 gcgggtcttt gccgatggct acctggacta ccaggcgaac gtctccctga cccagcccgt
     7321 ggcgctgaac gttcagctcc agcccaaacc cgtggtaccg gccacgatgg cggtggtcat
     7381 tccgaggccg ttcgtggact ccgacattcg gcccggggat ccccagagca ccatccggat
     7441 ctgggtggat gacaagctgg tcaaccccaa gcgggagctt gcggggatcc agatcccgcc
     7501 gggcaagcat cgggtccgga tcggttccgg ggcgctcatg gtccagctgg gcgactacgt
     7561 gttccagccg ggcatcaact acaccctgga gctcggcctt acggcggtag tgaagacgcc
     7621 gaccgcacag cagtaagggg ccgcgcgcaa ccggcggaca tagaacatcc ctgacgggat
     7681 cgggcgcctc gaggagggtt caccgcgagc cgcggtgaaa acctccccag aggcgctcgc
     7741 tgttcctggg tgctctcccg ccgaccggag cgtcggcgga tctttcgaac ccattttcga
     7801 cgaggaggtt ttcatgaggc gcgccttgat cgtcctgctc atcctttcac cgctcctggc
     7861 gctgtacgcg cagagcccct ccccggccgc gcaaggggac gccctgaacg acaaggggca
     7921 atacctggac gctcggaaga tcgagctgga cgccgcggcc gccgccagcc ccgcggagaa
     7981 ggcggagctc tattggcgcg cggcccgtga gaccatggag ctgggagatc tgtcggacaa
     8041 agccaggaaa ccgcaggcgg acaccctggc catcttcagc gaaggggagg ggtatgcgga
     8101 caaggc
//
LOCUS       DASRZS010000438         6546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4469887, whole genome shotgun sequence.
ACCESSION   DASRZS010000438 DASRZS010000000
VERSION     DASRZS010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4469887"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFH83_10705"
     CDS             complement(<1..287)
                     /locus_tag="VFH83_10705"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET6486881.1"
                     /translation="MADERETTLEDVGKLVLRITVAGLLLFHGVAKILHGIEGIRGDM
                     TRIHLPEILAYAVYIGEVIAPLFVLVGAWTRIAALIILIDLVVAVLLARMPA"
     gene            complement(334..1509)
                     /locus_tag="VFH83_10710"
     CDS             complement(334..1509)
                     /locus_tag="VFH83_10710"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6486882.1"
                     /translation="MREELALYGGKRTAPDPFPPWPQFAERTFLDVQEPLRSGRVAAW
                     SGERCRELEGRWAGWIGARFAVSCSSGTAALHLALLALGVGPGDEVILPSHTFFSTTL
                     AVLHAGARPVFCDVTEDQTIDPRGIPPLIGPRTKAIVAVHLYGVVCAVDRIRQIAQGA
                     GLRVVEDCAQCVGGELDGKMAGSLGDAGCFSFSEGKHISAAGEGGMVVTAQEDVAHAC
                     SSLRDYGRDPLVSSSPSHIHVGYNYRMTEIQAIVARNELDRLETWNLARRRGFARAYD
                     HAFSQLPGVKAIPLDSQHRRNAYWKYPLQVDLAHLACPGDEVRAAVSAEGVPGRLDAV
                     PESYEEPALAPYAGPRCRTAEALRQCTMLLSLPPSWERPHVELCVAAVKKVLRAYRR"
     gene            complement(1534..2298)
                     /locus_tag="VFH83_10715"
     CDS             complement(1534..2298)
                     /locus_tag="VFH83_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486883.1"
                     /translation="MAVYLYLSLIPEALVASMLSPMDFGAYFAVGTEKKARGQAMFFN
                     VDRGFKSDYFPMSEIDTRCVPHAKGEPKRSLYLSVYRALEHVPMSAIGNLYLATRDGR
                     VLEVAPSRSLPTYPRAYRMYQEIAPVDPRVVSLMDPREFAAFMTDQKRSIHVPKIVFA
                     ELGLGDLAENPETGTGRNLPYPRIDHLRNCLAELRVAQHKGTKTVDRIAPADFPFRMV
                     NSGFYLGDAHEVVHYPMPSEKELETTHHVWWRSASQ"
     gene            complement(2434..3456)
                     /locus_tag="VFH83_10720"
     CDS             complement(2434..3456)
                     /locus_tag="VFH83_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6486884.1"
                     /translation="MAEEPARVTINDGERVLPVRAGEPVLFALMGARIFVPSACGGRG
                     ICGQCRLVIPGARGGPTDAEIALVSPADRARGVRLSCQVPVVGEVAVRLPTGSLAARP
                     YRARVLEIRDETPDMRRLILELEEPGGMRFEAGQYVQLVRPGTEDDERPVYRAYSIAS
                     APSSTRLLTLLVARVAGEAGELTRFVFQELTEGRRVALNGPLGTFTLRPSRRELLFVA
                     GGSGYAPIRSILMDAAERGLPRPATLYFAARSLRDLFLLDEIRQIQRSLPALRFVPVL
                     SRPLPEDRWQGERGGIAAALARLLPTLDNHDVYLCGSPGMVDACIAALRSRGLAEDRL
                     VFDRFA"
     gene            3549..3932
                     /locus_tag="VFH83_10725"
     CDS             3549..3932
                     /locus_tag="VFH83_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486885.1"
                     /translation="MPNTLVSHALLQYLLIPLVVLWVYHLTQRRYETTGERKREATLL
                     LSLALIVCWGLAWAFQRFGVPDAYLALVVVALAVFLYAQRQRLFPYRFRCERCGVSLS
                     IQRILYIDHGLCASCEPAGKEGERD"
     gene            3929..5137
                     /locus_tag="VFH83_10730"
     CDS             3929..5137
                     /locus_tag="VFH83_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET6486886.1"
                     /translation="MTKNKRLSMPFWCVSNPVGDPFGPAVMDRISSVEVCDLLCKARG
                     EKLIDFTSAHDDDLVAWDPRNLEDDLDQSSPASQTLRAIKEKLEAAELKFMMITCGLH
                     NNPVFRNGGLTNPNPEVRMLAARKVMRTIRIGNYLGAEYLTYWVARDGFETQFAVPWE
                     RSYRYLIEGLNLAEKYAKEQNGTVRHGTIEPKPNEPRGEMFLPTTGHALGLIAMLSDP
                     SFWGVNPEILQHEGMTNLSAVVAVSMAAQAQKLFFLHVGNQKPGQFDNDMPVLVGMDG
                     LKEMVAALWLLDRMSWNGHVEFDNHILRTDTAPGKDNATRIRMDFIRQNVENYRMAER
                     KAHDLGADADLAKMMSGLWDREPGLARTLSHADTAEILRATVDYERVNGTGLQIGRLD
                     QMVNRRLLGL"
     gene            5134..6180
                     /locus_tag="VFH83_10735"
     CDS             5134..6180
                     /locus_tag="VFH83_10735"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET6486887.1"
                     /translation="MSRIAVIPGDGTGPEVVAEGVKVLKAAARTFKLTLDFTEFDWGG
                     SRYLKTGETLPSGAVETLRGFDAIYLGAIGHPDVKPGILEQGILLKLRFELDQYINLR
                     PVRLYPNVETPLANKGPADIDFLIVRENVEGLYVGSGGFLKKGTQDEVAIQEMIYTRR
                     GVERCVRFAFDLTRKRGKRRKLTLCGKTNVLTFAQDLWFRTFQEVAREYPDITTDYAH
                     VDATTMWMVKNPEWFDVIVTENMFGDIITDLGAMIQGGMGVAAGGNINPGGVSMFEPI
                     GGSAPKYTGQNVINPIAAILAGSMMLDQLGHGDAAAAVERSVVKVIGTMPSMAAGKMG
                     HGTKEIGDLVAAGV"
     gene            complement(6177..>6546)
                     /locus_tag="VFH83_10740"
     CDS             complement(6177..>6546)
                     /locus_tag="VFH83_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET6486888.1"
                     /translation="YKDYVGLDASMANLMRPAIYGAYHHITVLGKESQPADHLYDVTG
                     SLCENNDKFAIDRKLPRIDEGDVVVIHDTGAHGYAMGFNYNGKLRSAELLLGLDGSVR
                     QIRRAETLEDYFRTVEFPKA"
BASE COUNT         1161 a         2185 c         2210 g          990 t
ORIGIN      
        1 gccggcatcc gcgccaacag gacggccacc accagatcga tgaggatgat gagcgcggca
       61 atacgggtcc aggcccccac caggacgaac aggggggcga tcacctctcc gatgtagacg
      121 gcgtacgcga ggatctcggg aagatggatc cgggtcatgt ctccccggat gccctcgatc
      181 ccgtgcagga tcttggctac gccgtggaac aggagcaagc ccgcgacggt gatccgaagc
      241 acgagcttgc cgacgtcttc aagggtcgtc tccctttcat cggccattgg gttggaattt
      301 ggctgcggct ggatgcgaag tcaagcagac cgctcaacgg cgataggcgc gcaacacctt
      361 cttcacggcg gcgacgcaca gctcgacgtg cggcctctcc cagctcggcg gcaggctgag
      421 cagcatcgtg cactgccgga gggcttccgc ggtccgacac cgcgggccgg catagggggc
      481 gagcgcgggc tcctcgtagg attccggcac ggcgtccagc ctgccgggca ccccctccgc
      541 gctcaccgcc gcccgcacct cgtctcccgg gcaggccagg tgggccagat cgacctgaag
      601 ggggtacttc cagtaggcgt tcctgcggtg ctggctgtcc agcgggatgg ccttgacccc
      661 gggaagctgg gagaaggcat ggtcgtacgc tcgggcaaag ccgcggcgac gcgcgaggtt
      721 ccaggtctcc agccgatcca gctcgttgcg cgcgacgatc gcctggatct cggtcatccg
      781 gtagttgtag ccaacgtgga tgtgggaggg agaggaggac accaacgggt cacggccgta
      841 gtcccgaagg gagctgcacg cgtgggccac gtcctcctgg gccgtgacca ccataccgcc
      901 ctcccccgcg gcggagatgt gcttgccctc ggagaagctg aaacagcccg cgtcccccag
      961 agagcccgcc atcttcccgt ccagctcccc gcccacacac tgggcgcagt cctcgaccac
     1021 ccggagtccg gcgccctggg cgatctgacg gatccggtcc acggcacaga ccacgccgta
     1081 cagatgcacc gccacgatgg ccttggtccg cggcccgatg agcggcggaa tgccgcgcgg
     1141 gtcgatggtc tggtcctccg tcacgtcgca gaacacggga cgcgcgccgg cgtgaaggac
     1201 cgcgagcgtc gtggagaaga aggtgtgcga cgggaggatc acctcgtccc cgggtcctac
     1261 gccaagggcc agaagcgcca ggtggagagc cgcggtcccg ctggagcagc tcacggcgaa
     1321 ccgcgccccg atccacccgg cccagcgtcc ttccagctcc ctgcagcgct cccccgacca
     1381 ggcggccacc ctccccgacc tcaggggctc ctggacgtcc aggaaggtcc tctcggcgaa
     1441 ctgaggccag ggggggaacg gatcaggcgc ggtccgcttc cccccataga gggcaagctc
     1501 ttcgcgcacg agagccgcgc ggccgtccgc gccttactgg gacgccgacc gccaccagac
     1561 gtggtgggtg gtttccagct ccttctcgga aggcatgggg taatggacca cctcatgggc
     1621 atcgccaaga tagaagccgc tgttcaccat gcggaagggg aaatccgccg gcgcgatccg
     1681 gtccacggtc ttggtccctt tgtgctgggc cacgcgcagc tcggcgaggc agttgcgcag
     1741 gtggtcgatc cgcgggtatg ggagattgcg gccggttccc gtctcggggt tctccgcgag
     1801 gtcccccagt cccagctccg cgaacacgat cttggggacg tggatgctcc gcttctgatc
     1861 cgtcatgaac gccgcgaact ccctgggatc catgaggctc acgacgcgtg ggtccaccgg
     1921 cgcgatctcc tggtacatgc ggtaggccct cgggtaggtg ggcagcgacc gcgagggcgc
     1981 cacctccagc acccgcccgt ccctcgtggc gaggtacagg ttgccgatgg cgctcatcgg
     2041 cacgtgctcc aaagcccggt agaccgaaag gtacagcgag cgcttgggct cgcccttggc
     2101 gtgcggcacg caccgggtgt cgatctccga catggggaag tagtcactct tgaacccgcg
     2161 gtccacgttg aagaacatgg cctgaccgcg cgccttcttc tccgttccca cggcgaagta
     2221 ggctccgaag tccatcggcg acaacatcga tgccacaagc gcctctggaa tgagagagag
     2281 atacagatac actgccatac tcacctcccg cacacataat aacgcccgag agaaggcacg
     2341 gcaaatcgcc gtagaccgcc aaccgggctg ttcgacgacg agccggtccg tgaaccggcg
     2401 gtccgggaat cggcaggtcc ggcggaccgc tggtcaggcg aaccgatcga agaccagccg
     2461 atcctcggcg agccctcggg accgaagcgc ggcgatgcac gcgtccacca tgccggggct
     2521 cccgcagagg tagacgtcgt gattatcgag cgtcgggagg agccgcgcaa gggccgcggc
     2581 aatccctcct cgctcgccct gccagcggtc ctccgggagc ggacgggaga gcaccgggac
     2641 aaaccgaagc gcgggaagcg acctctggat ctgccggatc tcgtcgagca ggaacaggtc
     2701 ccggagcgat cgcgcggcga agtacagggt ggcgggacga ggcagaccgc gttccgcggc
     2761 gtccatcagg atggagcgga tgggcgcgta cccagagcca ccggccacga aaaggagctc
     2821 ccgccggctc ggccgcaggg tgaaggtacc cagggggccg ttgagcgcca cccgccgccc
     2881 ttcggtcagc tcctggaaca cgaaccgcgt cagctcgccg gcctcccccg ccacgcgcgc
     2941 taccagcagg gtcagcagac gcgtcgacga gggcgcggag gcgatggagt aggcgcgata
     3001 cacgggccgt tcgtcgtcct ccgtgccggg acgaacgagc tgaacatact ggcccgcctc
     3061 aaagcgcatt cccccaggct cctccagctc caggatgagg cggcgcatgt cgggagtctc
     3121 gtcgcggatc tccaggaccc tggcgcggta cggccgcgcc gccaggctgc cggtcggcag
     3181 acgtacggcg acctccccga ccaccggtac ctggcaggac agccgcacgc cgcgcgcgcg
     3241 atccgcgggg gacaccaggg ctatctccgc gtccgtggga ccgccgcggg cgcccgggat
     3301 gacgagccgg cactgaccgc agatcccgcg tcctccgcag gccgagggca cgaagatccg
     3361 ggcgcccatc agcgcgaaga gaacgggctc tcccgcgcgg accggcagga cgcgctcccc
     3421 gtcgttgatg gtcacgcgcg cgggctcttc ggccatgggc agaggatacg cgcggcccgc
     3481 cggccgggtc aacgcggccg gttgaccgcc cggtgtggcg ccagtagact ttgcagaacg
     3541 gagagagagt gccgaatacc ctggtgtccc acgcgctgct gcagtatctg ctgatccccc
     3601 tggtcgtcct gtgggtgtac cacctcacgc agcgccgcta tgagacgacc ggagagcgca
     3661 agcgcgaggc cacgctgctc ctctccctgg ccctcatcgt ctgctggggt ctggcgtggg
     3721 ccttccagcg cttcggcgtc cctgacgcgt acctggcgct ggtggtggtg gccctggccg
     3781 tgttcctgta cgcgcagcgg cagcggctct tcccgtatcg gttccgttgt gagcgctgcg
     3841 gcgtctccct ctcgatccag cgcatcctct acatcgacca tggattgtgt gcctcctgcg
     3901 agcccgcagg caaggaagga gaaagagatt gaccaaaaac aagaggctct cgatgccgtt
     3961 ctggtgcgtg tccaacccgg tgggggaccc gttcggccct gcggtcatgg accgcatcag
     4021 ctccgtggaa gtgtgcgatc tgctctgcaa ggcccgcgga gagaagctca tcgacttcac
     4081 ctccgcccac gacgatgacc tggtggcctg ggatccccgc aacctcgagg acgacctgga
     4141 ccagtcgagc cccgcctccc agacgctccg cgccatcaag gagaagctcg aagcggcgga
     4201 gctgaagttc atgatgatca cctgcggcct gcacaacaac ccggtcttcc gaaacggcgg
     4261 gctcaccaac cccaaccccg aggtgcggat gctggcggcc cgcaaggtga tgcggaccat
     4321 ccgcatcggc aactacctgg gcgccgagta cctgacgtac tgggtggcgc gggatggttt
     4381 cgagacccaa ttcgcggtgc cgtgggagcg aagctaccgc tacctgatcg agggcctcaa
     4441 cctggcggag aagtacgcca aggagcagaa cgggaccgtc cgccatggaa cgatcgagcc
     4501 caaacccaac gagccgcgcg gcgagatgtt cctccccacc acggggcatg ccctggggtt
     4561 gatcgccatg ctgagcgatc cttccttctg gggcgtgaac ccggagatcc tccagcatga
     4621 gggcatgacg aacctctccg ccgtggtcgc ggtttccatg gccgcgcagg cccagaagct
     4681 cttcttcctg cacgtgggca accagaagcc cggccagttc gacaacgaca tgccggtgct
     4741 ggtgggcatg gacggcctca aggagatggt ggccgcgctc tggctgctcg accggatgag
     4801 ctggaacggc cacgtggagt tcgacaacca catccttcgc acggacacgg cgccgggcaa
     4861 ggacaacgcc acgagaatcc gcatggactt catccgccag aacgtggaga actatcgcat
     4921 ggcggagcgc aaggcccatg acctgggcgc cgacgcggac ctggccaaga tgatgagcgg
     4981 gctgtgggac cgtgagccgg gtctggcccg gacgctctct cacgcggaca cggcggagat
     5041 cctccgcgcc acggtagact acgagcgggt caacggcacc ggcctccaga tcggccgtct
     5101 ggaccagatg gtgaaccgga ggcttctggg cctatgagca ggattgccgt cattcccggg
     5161 gacgggacgg ggcccgaagt ggtcgcggaa ggcgtgaagg tcctcaaggc cgccgcccgc
     5221 acgttcaagc tgaccctgga cttcaccgag ttcgattggg gcgggagccg ctacctcaag
     5281 acgggagaga ctcttccctc gggggcggtg gagaccctgc ggggcttcga cgccatctac
     5341 ctcggggcca tcggccatcc cgacgtgaag ccagggatcc tggagcaggg aattctcctc
     5401 aagcttcgct tcgagctgga ccagtacatc aacctccgtc cggtgaggct gtacccgaac
     5461 gtggagaccc cgctggcgaa caagggtccg gcggacatcg acttcctgat cgtgcgggag
     5521 aacgtggagg gcctgtacgt cgggtccggg gggttcctga agaagggcac ccaggacgag
     5581 gtggccatcc aggagatgat ctacacgcgt cggggcgtcg agcgatgcgt gcgcttcgcg
     5641 ttcgacctca cccgaaagcg cggcaagcgg cggaagctga ccctctgcgg caaaacgaac
     5701 gtcctcacct tcgcgcagga cctgtggttc cgcacgttcc aggaagtagc cagggagtac
     5761 ccggacatca ccacggacta cgcccacgtg gacgccacca ccatgtggat ggtgaagaac
     5821 ccggagtggt tcgacgtgat cgtaaccgag aacatgttcg gcgacatcat cacggacctc
     5881 ggcgccatga tccagggagg catgggcgtg gccgccggcg ggaacatcaa ccccggcggg
     5941 gtatccatgt tcgagcctat cggcggctcg gcgcccaagt acacgggcca gaacgtcatc
     6001 aacccgatcg cggccatcct ggcgggctcc atgatgctgg accagctcgg ccacggggac
     6061 gcggcggccg cggtggaacg ctccgtggtg aaggtgatcg gcacgatgcc cagcatggcg
     6121 gccgggaaga tgggccacgg cacgaaggag atcggcgacc tggtagcggc gggagtctag
     6181 gccttcggga actccaccgt ccggaagtag tcctccaggg tttcggcccg tcggatctgg
     6241 cggaccgagc cgtccaagcc caggagcagc tccgcggagc gcagctttcc gttgtagttg
     6301 aaccccatgg cgtagccgtg cgcgcccgtg tcgtggatca ccacgacgtc accctcgtcg
     6361 atccggggga gcttgcggtc aatggcgaac ttgtcgttgt tctcgcacag cgagccggtc
     6421 acgtcgtaga ggtgatccgc gggctgggac tccttgccca ggacggtgat gtggtggtag
     6481 gcgccgtaga tcgcgggccg catgaggttg gccatggaag cgtccagccc cacgtagtcc
     6541 ttgtag
//
LOCUS       DASRZS010000439         9544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5235631, whole genome shotgun sequence.
ACCESSION   DASRZS010000439 DASRZS010000000
VERSION     DASRZS010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9544
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5235631"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1042)
                     /locus_tag="VFH83_10745"
     CDS             complement(149..1042)
                     /locus_tag="VFH83_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HET6486889.1"
                     /translation="MSLSPLRDLLSAAVAGGYAIGYFEAWDQYSLEACLEAAEATRSP
                     AILGFGAAVADQAWLERWGIEELAGLARRLAEAASVPAAVLFNEARTIGQIRRGLDAG
                     CTAVMLDSSKFPLEENLAWTRKVVSMARPFDADVEVELGHLPDARDPSERASMTDPEE
                     AERFVRQSGVQALAVSVGSVHSLAAGGAPLDLERLAAIHAVVPVPLVLHGGTGIPPQL
                     VPKAAARGVAKVNYGMKMKLAYLDGMRTALAGAPPNPDVHALVGGRHQTDVLGRAQAR
                     VRELLVSLMRAYGSAGRADTP"
     gene            complement(1039..3639)
                     /locus_tag="VFH83_10750"
     CDS             complement(1039..3639)
                     /locus_tag="VFH83_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET6486890.1"
                     /translation="MEKQKITNGVFWVGIPEADLSIVCGCPADCVKHLMKSGLIVERE
                     KKGVLAQTGPNAILLSDTTIQKGSFSNLAEFPVLQMLYLQGMILPGHPNNTGRRPMLI
                     GLEDQVRSQSGYIFRGTYGLSTPEELMQAGLSEEQARDLLRIKRWFAFDSAQRTEDVL
                     DLRVVDRPAVELRGRAFVRRRGFNRYEFVHHGQSVTVDLNLDDSDGYPSTFQLGFHRI
                     RREYFSVIHIGEGDGWDTARPCMGSIVCFQGRLYLVDAGPGIQHSLTALGISANELSG
                     IFHTHAHDDHFAGLTSLAHAGRRIPYYATPAVRASVVKKYAALTGRAESTFQQFFEPR
                     DLTADAWNRINGLEVMPVLSPHPVETTVLFFRAFWDDGYRAYAHLADISSFEVLRKMV
                     TEDASRNGISQAFYESCTRRFLTPVDLKKVDVGGGLIHGRAEDFREDTSARILLSHTS
                     APLTDAQKEVGSSAVFAQSDVLISTPNDYLLLEARKHLESFFPSAHPSDLAMILNCPQ
                     EEFPPDTIILHAGSPVREVHLLLSGKAEMIESGSGLHNTLSAGALVGELECMAPERAH
                     ATCRATTHVTTLRIRSAMFREFAGRNGLLTSFHDTLERRRLLAGTWLFGEGVPYPILN
                     RLARTMERRFAREDDLLVPGERPEILVLSEGLATVFLGKRAIDNLRPGGFLGEDTVMR
                     GARELSPEMARRILEPTGERHLFRARALLDSTFYAIPGEAVADIPVVQWKLMETYERR
                     LKSYSAEIRFQWDDSYAVGVPEIDDQHRRIFEAVGGLENEAVEDGMEARVERLVTLVR
                     EHLDGAESAGRDGEFLRKIEGLRRYLTIAPGEALHTMVDNVKDWVMDHVLLEHGARYA
                     EAKAPVGQAS"
     gene            3840..4595
                     /locus_tag="VFH83_10755"
     CDS             3840..4595
                     /locus_tag="VFH83_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486891.1"
                     /translation="MDVRFLRMISAGRESLATGRVREDLRFLRERHGERIAALHPDTR
                     AQLGWVSVYPDRDASGAFYHLVGLDVSPTERASGLDLELLQEAGRLMREHRVRRLRFG
                     TSPLLTANASLYVTRLGARYRWKDGVRTPDGRPWPYVSCECDFDDPLERPLDLTEEEI
                     VPRSVLTWDGLHPSPRPRLVYSGPLSILLPDLDNRDLSDLAAADPGFLPTIYEAFHAL
                     SLHGYGFAWFDRLPSTRAESAGWYYVMRRTLTF"
     gene            4638..4907
                     /locus_tag="VFH83_10760"
     CDS             4638..4907
                     /locus_tag="VFH83_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486892.1"
                     /translation="MDTPVTIQLTRSERELILNVLHLSPEVQHKVANTRIAANRYSVE
                     FTQQELEGLLGGLGSEINATRDRTMAKAYEELRDRLEGLSGGAAT"
     gene            4947..5474
                     /locus_tag="VFH83_10765"
     CDS             4947..5474
                     /locus_tag="VFH83_10765"
                     /inference="COORDINATES: protein motif:HMM:NF021612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HET6486893.1"
                     /translation="MQGYSLYLFVLSVVAVAAVAAVVLLILLWRSERAHSRARIEELL
                     ESRQKSVEDARKESVRASRSSLKGKITEQLAPLLPGFLYEPADARFLGDPIDYVVFNG
                     YSRLRDEAPSAEPLDIVLLEVKQGGSTLSSFQKAIARCVTEGRVRFEICRVAEDGSVR
                     SEIWRPAAPGSREGA"
     gene            5471..6196
                     /locus_tag="VFH83_10770"
     CDS             5471..6196
                     /locus_tag="VFH83_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486894.1"
                     /translation="MRGWAVPVLSCLLMTAASLAIAQAAAGRPGREISLPPTAVKDTF
                     FAYVLGIIQTGVEVDIDNAGMRDILTEFKSTLKLPFDLVNRVTQHTADQSTSIGLDFD
                     GEVSIPIPFALLGYHPGSINSSPDLSFGVTKTVYQEPSAADASSPVFALTLQQGTVLV
                     NIDDWLEALFPSLLEDTWVRHIIFFVWKGQWVGLLEGRGQRDGREMRAYFNFTGNRIL
                     FPIPAELDRIGRRLVNQQVATAR"
     gene            complement(6224..6835)
                     /locus_tag="VFH83_10775"
     CDS             complement(6224..6835)
                     /locus_tag="VFH83_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HET6486895.1"
                     /translation="MAGKESDVRVQEVVVHVSARHCHLSTEHLRALFGPGASLTKLKD
                     LIQPGQFAANETVTIVGPKGSLEKVRILGPERGTTQVEISGTDQFVLGVQAPVRESGN
                     LEGSPGIQLKGPAGQVTLTSGLIRALRHIHMLPSDADRIGVRNGAHVSVRLVGDRATI
                     AENVLVRAVPNSALEMHIDTDEGNAAGVPATSTGQILIPMMAV"
     gene            complement(6884..7576)
                     /locus_tag="VFH83_10780"
                     /pseudo
     CDS             complement(6884..7576)
                     /locus_tag="VFH83_10780"
                     /inference="COORDINATES: protein motif:HMM:NF022179.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
     gene            7705..8457
                     /locus_tag="VFH83_10785"
     CDS             7705..8457
                     /locus_tag="VFH83_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006838620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2161 family putative PD-(D/E)XK-type
                     phosphodiesterase"
                     /protein_id="HET6486896.1"
                     /translation="MKRHSSFSEADLAAPLYQYLSGLGYTVRSEVKDCDIAAVKGEDL
                     LIVELKKSLSLSLIVQAAQRQRVTDLVYVAVPRPSNKWAWWQESRGVRHLLRRLEIGL
                     ILVSLDPERPPVEVVSHPAPFQRRKRPKSRRAVLTEISGRTADFNRAGSNRRKLVTAY
                     RESAIRIACCLAARGNLSPAELRRMGTGDKTQSILHFNAYGWFERVERGVYALTAQGR
                     AELEDYPVLKRRYYPRGRVKAPAPAAASASAS"
     gene            8503..8781
                     /locus_tag="VFH83_10790"
     CDS             8503..8781
                     /locus_tag="VFH83_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET6486897.1"
                     /translation="MTFEHVDGSDKGRIVLYALSTCMWCRMAKNLLKDLGVGFEYLDV
                     DTLQAEEKETVKKEIRRWNPSGSYPTIVINDKECVSGFDETEIREKIK"
     gene            8778..9371
                     /locus_tag="VFH83_10795"
     CDS             8778..9371
                     /locus_tag="VFH83_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HET6486898.1"
                     /translation="MNAVPDDQVDRLHARLDQEAERGGYHLNPNREFTRELIRGLLVN
                     EERYGYWACPCRLADGEKAKDLDIICPCDYRDPDLSQFGTCFCGLYVSQEVKDGKRKF
                     ARIPERRPVERMMEEPAGPPSSGQPQAGAASAQSQSSTPSQTSQTPPPAPGLPNPTGL
                     SYPVWRCRVCGYLCARGEPPETCPVCRARKERFERFL"
BASE COUNT         1546 a         3268 c         3173 g         1557 t
ORIGIN      
        1 gtggcggtgt tggcggcgca cccgcccgta tccaacagca tgccgtccac tttgagcagc
       61 tcaccctcgg acggcagccg gggcaggagc ggcaggaaaa ggtccgccac caggatcccg
      121 gcgcacagca catcgaagcc gtcgtccctc atggcgtgtc cgccctgccg gcgctcccat
      181 aggcccgcat gagactcacc agcagctcgc gcacgcgcgc ctgcgcccgc cccaggacgt
      241 ccgtctggtg tctgccaccg accagcgcat ggacgtccgg attgggcggc gcgccagcca
      301 gtgccgtccg cattccgtcc aggtacgcga gcttcatctt catcccgtag ttcacctttg
      361 ccacaccacg ggcggcggcc ttggggacga gctgcggagg aatgccggtg cccccgtgaa
      421 gcaccagagg aacgggaacc accgcgtgga tggccgccag gcgctcgaga tccagcggcg
      481 cgccgcccgc ggccagggag tgcacgctgc ccacggatac cgccagggcc tggacgccgc
      541 tctggcggac gaagcgctcc gcctcctcgg ggtcggtcat ggaggcgcgc tccgacggat
      601 cccgcgcgtc cggcaggtgc cccagctcca cttcgacgtc cgcgtcgaac ggacgcgcca
      661 tggacacgac cttccgcgtc cacgccagat tctcttccag ggggaacttc gagctgtcca
      721 gcatcaccgc cgtgcagccc gcgtccagac cccgccggat ctggccgatg gtccgtgcct
      781 cgttgaacag caccgcggcc ggcacggagg cggcttcagc gaggcgacgg gccagacccg
      841 ccagttcctc gatcccccac cgttccagcc atgcctggtc cgcgacggcc gcgccgaacc
      901 caaggatggc cggtgaccgc gtggcctccg ccgcctccag acacgcctcc agggagtact
      961 gatcccatgc ctcgaagtac cctatcgcgt agcctccggc cacagccgcc gaaagcaggt
     1021 cccgcaacgg gctgaggctc atgaggcttg cccgacggga gcctttgcct cagcgtagcg
     1081 cgccccgtgc tccaggagca cgtggtccat cacccagtcc tttacattgt cgaccatcgt
     1141 gtgaagcgct tcgccgggcg cgatggtgag gtagcgccgc agaccctcga tctttctgag
     1201 gaactcgccg tcgcgacccg cggattccgc gccgtccagg tgctcgcgaa ccagggtcac
     1261 gagccgctcc acgcgggcct ccatcccgtc ctcgacggcc tcgttttcca gccctccaac
     1321 ggcctcgaag atgcgccgat gctggtcgtc gatctctggg acgccgaccg cgtaggaatc
     1381 gtcccactgg aaccggatct cggcgctgta gctcttcagt cgcctttcgt aggtttccat
     1441 gagcttccac tgaaccaccg gtatgtcggc cacggcctct cccggaatgg cgtagaaggt
     1501 ggaatccagc agggcgcggg ctcgaaagag atgacgctca ccggtcggct ccaggatccg
     1561 ccgggccatc tcgggagaaa gctcccgcgc cccccgcatc acggtgtctt cccccaggaa
     1621 gccgcccggc ctcaggttgt cgatggcccg cttgcccagg aacaccgtcg ccagcccttc
     1681 ggagaggacc aggatctccg gccgttctcc cggcaccagc aggtcatcct cccgcgcgaa
     1741 ccgccgctcc atggtccggg ccagccggtt caggatgggg taggggaccc cctcgccgaa
     1801 gagccaggtg ccggccagga gccttcgacg ttccagcgtg tcgtggaacg acgtgagcag
     1861 accgtttcgg ccggcgaact cccggaacat cgcgctgcgg atcctcagcg tggtcacgtg
     1921 cgtcgtggcc cggcaggtcg cgtgcgcgcg ttcgggcgcc atgcactcca gctcacccac
     1981 cagcgcgccc gcggacaggg tgttgtgcag gccgcttccc gactcgatca tctccgcttt
     2041 gccgctgagc agcagatgca cctctcgcac gggagagccc gcgtgcagga tgatggtatc
     2101 cggagggaac tcctcctggg ggcagttgag gatcatggcc aggtccgaag ggtgggccga
     2161 gggaaagaag ctctccaggt gtttgcgggc ttccaggagg aggtagtcgt tcggcgtgga
     2221 gatgagcacg tccgactggg caaacacggc ggaagagccg acctctttct gcgcgtccgt
     2281 caacggcgcg gaggtgtggc tcaggaggat ccgcgcggag gtatcctccc gaaaatcctc
     2341 ggcgcgtccg tggatcagcc cgccgccgac gtctactttc ttcagatcca ccggcgtcag
     2401 gaaccgtcgg gtgcagctct cgtagaaagc ctgggagatc ccgttgcggg aggcgtcctc
     2461 cgtcaccatc ttgcggagca cctcgaagga gctgatgtcc gccaggtgcg cgtaggcgcg
     2521 gtacccgtcg tcccagaacg cgcggaagaa gagcacggtg gtctccacgg ggtgcggcga
     2581 gagcacgggc atgacctcaa gaccgttgat ccggttccac gcgtccgccg tgaggtcccg
     2641 cggctcgaag aactgctgga aggtcgactc ggcccttccg gtgagcgccg catacttctt
     2701 caccacgctc gctcgcaccg ccggggtggc gtaatagggg atcctgcggc ccgcgtgggc
     2761 cagggaggtg agtcccgcga agtgatcgtc gtgcgcgtgg gtgtggaaga tcccggagag
     2821 ctcgttggcg ctgatcccca gcgccgtcag ggagtgctgg atgccgggcc ccgcatcgac
     2881 caggtagagc ctcccttgaa agcagacgat gctccccatg cacggccggg cggtgtccca
     2941 cccgtcgccc tccccgatat ggatcacgga gaagtattcc cgacggatgc ggtggaaccc
     3001 gagctggaag gtggatgggt atccgtcgga gtcgtccagg ttcaggtcca cggtcaccga
     3061 ctgcccgtgg tggacgaact cgtagcggtt gaagccgcgt cgtcgcacga acgcccgccc
     3121 gcgcagctcc acggccggcc gatccaccac ccggaggtcc aggacgtcct ccgtgcgctg
     3181 cgcgctgtcg aacgcgaacc accgcttgat gcgcaggaga tcccgcgcct gctcctcgga
     3241 gagaccggcc tgcatgagct cctccggggt ggacaggccg taggtgcctc ggaagatgta
     3301 gccgctctgg ctgcggacct ggtcctccag cccgatgagc atcggccggc ggccggtgtt
     3361 gttcggatgg cccggcagga tcatcccctg caggtacagc atctggagca cggggaactc
     3421 cgccaggttc gagaagctgc ccttctggat cgtggtatcc gacagaagga tggcgttggg
     3481 ccccgtctgg gccaggacgc ccttcttctc gcgctccacg atgaggccgc tcttcatcaa
     3541 gtgcttcacg cagtccgcgg ggcacccgca gacaatcgag agatccgcct cggggatacc
     3601 gacccagaag accccattcg tgatcttctg cttttccatc cgacaacctt tccatgggga
     3661 cgcggccatt ccccgcggcg tagagcgcgc cgcgcaggag ggggcgcgtg cgccccgcgg
     3721 cgcggcattc gccccgccct cgggcccggc gtcctcagcg ttcgggtgta gtatatccac
     3781 ggcgcgggcc attgcggaag tgcggcgccc cgccgatagt tggggtagac ggtgcgggaa
     3841 tggacgtccg tttcctgcgc atgatatccg cgggtcgaga gagtctggcc accggccggg
     3901 tgcgcgagga cctgcgtttc ctccgtgaga ggcacgggga gaggatcgcc gcgttgcatc
     3961 ccgatacgcg cgcccagctc ggctgggtca gcgtgtaccc ggaccgggac gcgagcggcg
     4021 ccttctacca cctggtgggt ctggacgtct ctccgacgga gcgggcctcc ggcctggacc
     4081 tggagctcct ccaggaagcc ggacgtctca tgcgggagca ccgcgtgcgt cggctgcgct
     4141 ttggcaccag tcccctcctg acggcgaacg cgtccctgta cgtgacacga ctgggcgcgc
     4201 gctaccggtg gaaggatggg gtccggacgc cggacggcag gccgtggccc tacgtgagct
     4261 gtgagtgtga cttcgacgat ccgctggaac ggccgctcga cttgacggag gaggagatcg
     4321 tgccgcgcag cgtgctgacc tgggacggcc tccatccgtc gcctcgcccg cggctggtct
     4381 actccggtcc gctctccatc ctcctgccgg acctggacaa ccgcgatctc tccgacctgg
     4441 cggcagcgga tccgggcttc ctccccacca tctacgaggc gtttcacgcg ttgtctctcc
     4501 acggatacgg gttcgcgtgg ttcgaccgcc tgccgtcgac ccgggccgaa tcggcgggat
     4561 ggtactatgt gatgaggcgc acgctcacct tctagcgctc ccttgtcgcc cccaccccgc
     4621 cggggtagta tcgatccatg gacaccccag tgacgatcca gcttacccgt agcgagcgcg
     4681 agttgatcct gaacgtcctg cacctctccc ccgaggtgca gcacaaggtg gccaacaccc
     4741 ggatcgcggc caatcggtat tccgtcgagt tcacccagca ggagctggag gggctcctgg
     4801 gcgggctcgg ctccgaaatc aacgccaccc gcgaccggac catggccaag gcttacgagg
     4861 agctgcggga tcggttggaa gggctctccg ggggcgccgc cacctagcgc cggctcgtcg
     4921 agtcgccgga cgcgcctccg ccaggcatgc agggctattc gctctacctg ttcgtactct
     4981 ccgttgtcgc cgtggcggca gtggcggccg tggtcctgct gatcctcctc tggagatcgg
     5041 agcgcgcgca cagccgcgct cggatcgaag agctcctgga gagccgccag aagagcgtgg
     5101 aggatgcaag gaaggaaagc gttcgcgcca gccgcagctc gctcaaagga aagattacgg
     5161 agcagctcgc tccgctgctc cctggcttcc tgtacgagcc ggcggatgcc cggttcctcg
     5221 gggatcccat cgactatgtc gtgttcaacg ggtattcccg cctccgggac gaggcaccct
     5281 ccgcagagcc cctggacatc gtgcttctcg aggtgaagca aggcggctcc accctttctt
     5341 catttcagaa agccatcgcg cggtgcgtga cggagggccg ggttcggttc gagatctgta
     5401 gggtggcgga ggacgggagc gttcgcagcg agatctggcg acccgcggcg ccgggaagca
     5461 gggagggagc gtgaggggat gggccgtccc ggtcctgtcc tgtctgctca tgaccgcggc
     5521 gtccctggcc atcgcccagg ccgcggcggg gcggccgggt cgggagatca gcctcccgcc
     5581 cacggcggtg aaggacacct tcttcgccta cgtgctgggg atcatccaga ccggcgtgga
     5641 agtggatatc gacaacgccg gcatgcggga catcctcacg gagttcaaga gcaccctgaa
     5701 gctgcccttc gacctggtaa accgggtgac gcagcatacg gccgaccaga gcacctccat
     5761 cggactggat ttcgatggag aggtgagcat cccgatcccg ttcgccctgt tgggctatca
     5821 ccccggcagc atcaactcga gccctgacct gtccttcggg gtcaccaaga ccgtctacca
     5881 ggaaccgtcg gctgctgacg cgtcctcgcc ggtattcgcg ctcactctgc aacagggcac
     5941 ggtgctggtg aacatcgatg actggctgga ggcgctcttc ccctccctgc tggaggacac
     6001 ctgggtgcgc cacatcatct tcttcgtctg gaagggtcag tgggtcgggc tcctggaggg
     6061 ccgaggtcag cgcgacggcc gtgagatgag ggcgtacttc aacttcaccg gcaaccgcat
     6121 tctctttccc atcccggccg agctggaccg gatcgggcgg aggctggtca accagcaggt
     6181 ggccacggcg cggtgatgcc gcggggatcc gccgggcgcc tcgctacacc gccatcatcg
     6241 gaatcaggat ctggcccgtc gaggtggcgg ggacgccagc cgcgttcccc tcgtcggtgt
     6301 cgatgtgcat ctccagcgcg gaattgggaa ccgcccggac caggacgttc tccgcgatgg
     6361 tggcgcgatc ccctaccagc cgcaccgaaa cgtgcgcgcc gttccgcaca ccgatgcgat
     6421 ccgcgtccga cgggagcatg tggatgtggc gcagcgcccg gatgagaccg gaggtcaggg
     6481 tcacttgccc ggcaggcccc ttgagctgaa tgccggggct gccctccagg ttcccagact
     6541 cccggacggg agcctgaacc cccagcacga actgatccgt cccggagatc tccacctggg
     6601 tggtgccgcg ctcgggtccc aggatgcgaa ccttctccag ggaccccttc ggcccgacga
     6661 tcgtcaccgt ctcgttcgcc gcgaactgcc ccggctgaat gagatccttc agcttggtga
     6721 gggaggcgcc ggggccaaag agcgcgcgca ggtgctccgt cgacaggtgg cagtgccgcg
     6781 cggagacgtg gaccaccacc tcctggaccc ggacatcgga ctccttgccc gccaggatct
     6841 cgtaggagaa ccgggaccag caggaggcct tggggttggc ccgcacctgg gtcagggccg
     6901 cacggacgcg ctcccgcgcc aggggtccgt actccttgcg gaaccgcagg tagtactcga
     6961 ggatcatgga gttctcactg acatagcggc cggggacgga gttcagggcc agggcaaaga
     7021 cgttgcacca gcactggctg caggtgcata gaccgtccgc gcgcagggct tccccgcact
     7081 cctctccgac gaccagctcc atgatgttga agggcgcgaa ggtggaccag accacgcccc
     7141 gcaccgactg cgccgtgcgt atggaggcca gcacgctggc ggtccgcagg cgctcggggc
     7201 tgcagagggc aagctccgcc accgcttctt cgatccgttc accggagtgc gcggtcctct
     7261 tgcggcagcg ctggaggtcc tccgtggaga tccagcccag ggacaccagc atctggccgg
     7321 catcggagcg atcgacgacg tgctgggact ggacccgcag cgcccgcgtg acctgttccg
     7381 cctcggccca gccctttcgg accgcgatct cccccagccg ggagcgcatg ccgacccgcg
     7441 ccgtgtcccg ctgctccgca agcagggagt ctacctgctc cggccgcaag gcggacgcct
     7501 ccacgagcag ctcgccgatc ttgggtaccg cctcgatctc gctcacgccc atggctgccc
     7561 tcctgttggg gcccatggtg gaccgggtcc gtacatccgg tcaagggaaa aacacgctgg
     7621 ggcacgctgg gggcgccccg aaggctggcg ccggggcttg atccggcgct tgagggcgga
     7681 ctaccctggc acgacgtgca ccccgtgaaa cgccatagct ccttctccga agcggatctc
     7741 gccgctcccc tctaccagta cctctcgggg ctggggtaca cggttcgaag cgaggtgaag
     7801 gactgcgaca tcgcggcggt caaaggcgaa gacctcctga tcgtcgagct caagaagagc
     7861 ctgagcctgt ccctcatcgt gcaggctgcc cagaggcagc gggtgacgga cctcgtttac
     7921 gtggcggttc cccgccccag caacaagtgg gcgtggtggc aggagagccg aggggtgagg
     7981 cacctcctgc ggcgcctgga gatcgggctg atcctcgtgt ccctggaccc cgagcggccg
     8041 cccgtggaag tggtgtccca cccggcgccg ttccagcgac gcaagcgtcc caagtcccgt
     8101 cgggcggtgc tcacggagat ctccggccgg acggcggact tcaaccgggc gggcagcaat
     8161 cggcgcaagc tcgtcaccgc gtatcgggag agcgctatcc ggattgcctg ctgtcttgct
     8221 gcccgcggca acctgtcccc ggcggagctc cgccgcatgg ggaccgggga caagacccaa
     8281 tccatcctgc acttcaacgc ctacggctgg ttcgaacgcg tggagcgggg agtctacgcg
     8341 cttaccgcgc agggccgggc ggagctcgaa gattaccccg ttctgaagag gcgctactac
     8401 ccgagaggcc gcgtgaaagc cccggcgccg gctgctgcgt ccgcctccgc gagttgaaac
     8461 cgcgcgctct tcggggctat gcttggaccc aaggagggtg tcgtgacgtt cgagcacgtt
     8521 gatggttcgg acaagggccg gatcgtcctg tacgccctga gcacatgcat gtggtgccgg
     8581 atggccaaga acctcctcaa ggacctcggg gtcgggttcg agtacctcga cgtcgacacg
     8641 ctccaggctg aagagaagga aaccgtgaag aaggagatcc gccggtggaa ccccagcggc
     8701 tcctatccca cgatcgtcat caacgacaag gaatgcgtct ccgggttcga cgagacggag
     8761 atccgggaga agatcaagtg aacgcggttc cggatgacca ggtggatcgc ctgcacgcgc
     8821 ggctcgacca ggaggccgaa cggggcggct accacctcaa ccccaaccgc gagttcacgc
     8881 gggagctgat ccgcggtctg ctggtcaacg aggagcgcta cgggtactgg gcctgtccgt
     8941 gtcggctcgc cgatggggag aaggccaagg acctggacat catctgcccg tgcgactatc
     9001 gcgatccgga cctatcccag tttgggacct gcttctgcgg cctctatgtc tcccaggagg
     9061 tcaaggacgg caagcggaag ttcgcgcgga tcccagaacg gcgaccggtg gagcggatga
     9121 tggaagagcc ggcagggccg ccgtcttcgg gccagccgca ggcgggcgcg gcgtccgccc
     9181 agtcccaatc gtccactccc tcccagacgt cccagactcc gccgccggcg ccgggcctgc
     9241 ccaaccctac cggtctcagc tatccggtct ggcgctgtcg agtgtgcggc tacctctgcg
     9301 ctcgaggcga gccgccggaa acctgcccgg tctgcagagc caggaaggaa cgtttcgagc
     9361 gattcctata agcccggatt ttcgcccggt accgagcctg catcctggct cgcgccgggg
     9421 ccgggcgcat cctcttccgg cagagcatcc agggtcccgg ccacggcgcg gtcctcttcg
     9481 ataagggctg ccaggtgctg ctgcgcggag gccgagtcca cggactgcac gatccggacc
     9541 agtt
//
LOCUS       DASRZS010000440         4983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1394384, whole genome shotgun sequence.
ACCESSION   DASRZS010000440 DASRZS010000000
VERSION     DASRZS010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394384"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VFH83_10800"
     CDS             complement(<1..489)
                     /locus_tag="VFH83_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HET6486899.1"
                     /translation="MSSAAAGGTVREAIPSHNEEAEVATLGAVLIDPEALPAIIALVR
                     PEDFYRGAHQRIFEAVLALFERGQSIDLITVTEELRSRAALEQCGGSAYVSRLTSAVP
                     TSANVEYYARIVQAASIRRTLAKVSREISIQAHDESTQIQVILEEAERRIFEISDRNQ
                     TGT"
     gene            complement(524..1138)
                     /gene="rplI"
                     /locus_tag="VFH83_10805"
     CDS             complement(524..1138)
                     /gene="rplI"
                     /locus_tag="VFH83_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET6486900.1"
                     /translation="MKVILNQDVVGLGEEGDIKEVASGYGRNYLLPRKLAVLHSKENL
                     ANLERRRTAIEKRKQEKRVEAQSLKERLEAEEVRIVAPAGENGKLFGSVNNSSVAVEL
                     EKKGFQIEKKRIEIPEHTIRNIGQYKVRIRLYDKEEASVRLIVEGAPQPGSSATASRT
                     RQSRARPAAESAPEPAAAQPAVPVSAPAAEPAPEAAESEDSQGS"
     gene            complement(1196..2206)
                     /locus_tag="VFH83_10810"
     CDS             complement(1196..2206)
                     /locus_tag="VFH83_10810"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HET6486901.1"
                     /translation="MYRRSSGAGIPEILLFTISSLVLYYLGVGVALFLVPLQILASRR
                     GPSTLLVACLFFLACFAGLRLAPLLFSRGAAQPGILTLLEMVIVAGLMGGLLVVNLSL
                     TRWKRTLYKISAATALAGVVAAALGWWLSSSASFHQAMQQLYAEISQTLSSILVPAGG
                     ASGSAVQSLFSPATLQKLSQAYLVRTVLLDVLVFLSFSWWLGQALSSRALAVLGKPVF
                     RFSAFHLESFWLWPLILCGAVVLLDLFFGLEPWSYAAWNVGLAVLFLYGLQALAIVRF
                     VFEKYRVPRLLWVLLLVGLAVLCASPQVGLYVLLIIPTFGVSEQWVRYRIPREPMPTE
                     RQ"
     gene            complement(2245..2691)
                     /gene="rpsR"
                     /locus_tag="VFH83_10815"
     CDS             complement(2245..2691)
                     /gene="rpsR"
                     /locus_tag="VFH83_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009851496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET6486902.1"
                     /translation="MSERPEAPRPEAPRPEAPRPEGPRAEGPRPEGPRPEGPRPEGGR
                     PDSGRFEGPRREGGYRSDRGDRGDRGGFQGRGRHKTFFKRKVCKFCIKKATIDWKDGS
                     SLKRFTSERGKIMPRRITGTCAKHQRELARAIKRARSLAMLPYVSR"
     gene            complement(2787..3230)
                     /gene="ssb"
                     /locus_tag="VFH83_10820"
     CDS             complement(2787..3230)
                     /gene="ssb"
                     /locus_tag="VFH83_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET6486903.1"
                     /translation="MADINHVVLVGRLTRNAELKYTNSGAAVSKFSIAINQRRKKDDQ
                     WVDEVNFFDIVLWGKSAESINQYLVKGKQVGIEGQLRQDRWEQDGQSRSKVEIVAGNV
                     QLLGGGGGGAGGGRGGAAPEGRPAGQPTTEGGAPAGDQFEDDIPF"
     gene            complement(3252..3533)
                     /gene="rpsF"
                     /locus_tag="VFH83_10825"
     CDS             complement(3252..3533)
                     /gene="rpsF"
                     /locus_tag="VFH83_10825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET6486904.1"
                     /translation="MRTYEAVVIFQSDEAQHQAGREFLRKEFESSGITIKKEEDIGDR
                     LLAYPVRKNDRGRYVLYLVDSEAEQLRTLNRTLKLRPEILKYLFVRKDE"
     gene            3663..4118
                     /locus_tag="VFH83_10830"
     CDS             3663..4118
                     /locus_tag="VFH83_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486905.1"
                     /translation="MTRRINYEDDIFTLSLQVRCLQDTLKLEIDPELFRERVLGDIAW
                     IDATIGRIYQSLREGSRYVKRKEHLRDLGRLKGTLADTLDALAESAVPFSGHVKDRLD
                     SIRQIRDAHRRDVLEIKTMLAGTDAPEQEHMVSAEELNLLMSAPDDEES"
     gene            4275..4592
                     /gene="rplU"
                     /locus_tag="VFH83_10835"
     CDS             4275..4592
                     /gene="rplU"
                     /locus_tag="VFH83_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET6486906.1"
                     /translation="MYALVDILGKQYKVEQGALLKVDHLPQDAGSAVEFSSVLLISDG
                     ASTKIGSPYVSGVSVRAVVESHGRDRKLVFGKYKKKKNYRRRYGHRQEYSLIKVEGFA
                     GVN"
     gene            4629..4982
                     /locus_tag="VFH83_10840"
     CDS             4629..4982
                     /locus_tag="VFH83_10840"
                     /inference="COORDINATES: protein motif:HMM:NF016233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HET6486907.1"
                     /translation="MPKMSATLRIHVSLSPEGLLRGFEARGHAGRAPAGENLACAAAT
                     VLLRSAARACAARGLATGGGAGEPGELTLSLARASEPEPGWLRGVTDMLLRGVDDLQR
                     DHPGAIEVELEGVEP"
BASE COUNT          836 a         1646 c         1642 g          859 t
ORIGIN      
        1 cgtgccggtc tggttgcggt ccgagatctc gaagatccgc cgctccgcct cctccaggat
       61 gacctggatc tgggtcgatt cgtcgtgcgc ctggatcgag atctcccgcg agaccttggc
      121 cagggtccgt cggatgctgg cggcctggac gatgcgggcg tagtactcga cgttggcgct
      181 ggtgggtacc gctgaggtga ggcggctgac gtaggcgctg ccaccgcact gctccagggc
      241 ggcgcgggag cgcagttcct ccgtcaccgt gatgaggtcg atggactgtc cgcgctcgaa
      301 cagcgcgagg accgcctcga agatccgctg gtgcgctccg cggtagaagt cctccggacg
      361 caccaacgcg atgatggcgg gcagggcttc cggatcgatg agcaccgcgc cgagcgtggc
      421 tacctccgcc tcctcgttgt gggaggggat ggcttcgcgg accgtgcccc cggcagcggc
      481 actgctcacc ttacagcctc acccgcacga cgcgcgccag ggatcaggag ccctggctgt
      541 cctcgctctc tgccgcttcc ggggcaggct ccgcggcagg cgcggaaacg gggacggccg
      601 gctgcgcagc ggcaggctcc ggcgcacttt ccgcggccgg gcgtgcccgg ctctggcggg
      661 tgcgggatgc ggtcgccgag gagccgggct ggggagcgcc ctccacgatg aggcggacgg
      721 atgcctcctc cttgtcgtag agacggatcc ggaccttgta ttgcccgatg tttcggatgg
      781 tgtgctccgg gatctcgatc ctcttcttct cgatctggaa gcccttcttc tccagctcca
      841 cggctaccga ggagttgttc acggagccga agagcttgcc gttctcaccc gccggggcca
      901 cgatccggac ctcttccgcc tccagccgct ccttgaggct ctgcgcctcc acccgcttct
      961 cctgcttgcg cttctcgatg gccgtccggc gtctctcgag gttggcaagg ttctccttgg
     1021 agtgcaggac cgccagcttc ctgggcagga ggtagttcct gccgtagccc gacgcgactt
     1081 ccttgatgtc tccctcttct cccagtccta ccacgtcctg gttgaggatc accttcatgc
     1141 ggaactcctt tccttcacgg gcgacacgcg gggcattccg cgcgctgcat gtccctcatt
     1201 gtctttccgt cggcatcggc tctcggggga tccgataccg cacccactgc tccgaaaccc
     1261 cgaacgtcgg gatgatcagg agcacgtaca accccacctg gggtgaggcg cacagtaccg
     1321 ccaggccgac caggagcagc acccagagca gccgcgggac ccggtacttc tcgaacacga
     1381 agcggacaat ggccagggcc tggaggccgt acaggaacag gaccgcgaga cccacgttcc
     1441 acgccgcgta ggaccagggc tccaggccga aaaacaggtc gaggagcacg acggccccgc
     1501 acaggatgag gggccacagc cagaagctct ccaggtggaa ggccgagaag cggaacacgg
     1561 gcttcccgag aacggccaga gcgcgggacg acagcgcctg ccccagccac caggagaacg
     1621 acaggaacac cagcacgtcc agaaggaccg tccgcacgag gtatgcctgg gacagcttct
     1681 gcagcgtggc cgggctgaac agcgactgca cggcggaccc cgaggcgcct cccgcgggaa
     1741 caaggatgga ggagagcgtc tgggagatct ccgcgtagag ctgctgcatg gcctggtgga
     1801 agctcgcgct cgaggaaagc caccacccca aagctgcggc gaccacgccc gccagcgccg
     1861 tggccgcgga gatcttgtac agcgtccttt tccaacgcgt caggctgagg ttcaccacca
     1921 gcaggccgcc catcagacct gctacgatga ccatctccag cagggtcagg atccccggct
     1981 gggcggcgcc ccgggagaag agcaggggcg ccaggcgaag gcccgcaaag caggctagaa
     2041 agaacaggca cgctaccagg agggtggacg gccctctgcg ggatgccagg atctgcaggg
     2101 gaacgagaaa cagcgctact ccaaccccca ggtaatagag gacgagcgag gagatggtga
     2161 aaaggagtat ctcaggtatg ccggcgcccg acgatctacg gtacatccac acccaaccac
     2221 ggaccggaat ctccgaccgg cggcctaccg ggatacgtag ggcagcatgg ccagagagcg
     2281 cgcccgcttg atggctctgg cgagctcccg ctgatgcttg gcgcaggtgc ccgtgatccg
     2341 ccggggcatg atcttgcctc tctccgacgt gaagcgcttc agggacgacc catccttcca
     2401 gtcgatggtc gccttcttga tacagaactt gcagaccttc cgcttgaaga acgtcttgtg
     2461 ccggccgcgg ccctggaagc cgccacggtc cccccggtct ccccggtcgg accggtatcc
     2521 accctcacgg cgaggcccct cgaagcggcc ggaatccgga cggccccctt cgggacgcgg
     2581 gccttccgga cggggtcctt cgggacgcgg gccttccgcg cggggaccct cgggtcgcgg
     2641 ggcttcgggc cgcggggctt cgggccgcgg ggcttcaggc cgctcgctca tggccggcgc
     2701 gggctcggcc gcgggttgcg aaccacccgc ctcgggtcct cgggtctcat tgctatccga
     2761 catggttgct atcctctctc ctctggctaa aagggaatgt catcctcgaa ctggtcgccc
     2821 gctggggctc ccccctccgt ggtcggctgg cccgcgggtc tgccctctgg ggccgcgcct
     2881 ccacgaccac cgccagcgcc gcccccgccg ccacccagga gctgtacgtt gccggccacg
     2941 atctcgacct tgctgcgcga ctggccgtcc tgctcccagc ggtcctggcg gagctggccc
     3001 tctatcccca cctgcttgcc tttcaccagg tactggttga tcgactccgc ggacttcccc
     3061 cacaggacga tgtcgaagaa gttgacctca tccacccact ggtcgtcctt cttgcggcgt
     3121 tggttgatgg cgatgctgaa cttgctcacc gcggcgccgc tgttggtgta cttcagctcc
     3181 gcgtttcggg tgagcctgcc gaccagaacg acgtggttga tgtccgccat gacgccctcc
     3241 cggggaaatg gctactcgtc cttgcgaacg aaaaggtatt tcaggatttc gggccgcagc
     3301 ttcagcgtgc ggttcagggt gcgaagctgc tccgcctcgc tgtccaccag atacaggaca
     3361 tagcgtcctc ggtcgttctt gcgcacgggg taggcgagca gccgatcgcc gatgtcctct
     3421 tccttcttga tcgtgatccc agagctctcg aactccttgc gcaggaactc gcgaccggcc
     3481 tggtgctggg cctcgtcact ctgaaagatg acgaccgctt cgtaggtcct cactctgtca
     3541 ttcctcctcg tggacataag gctcacgctc ggggcgtgag caaagaggga gtagtcaaac
     3601 tacccaaggg aggtcatcct gtcaagggtg tccggccgaa ggccgatcct tagagggaag
     3661 cgatgacgcg acgaatcaac tacgaggacg acatcttcac cctgtccctg caggtgcgct
     3721 gtcttcagga cacgctcaag ctggagatcg accccgagct gttccgcgag cgggttttgg
     3781 gggacatcgc gtggatcgac gcgaccatcg gccgcattta tcagagcctc cgcgagggct
     3841 cgcggtacgt caagcgcaag gagcacctgc gcgacctggg ccggctcaag ggcacccttg
     3901 cggacacgct ggatgcgttg gcggaatccg ccgttccgtt ctcgggccac gtcaaggacc
     3961 ggctggactc gatccgccag atccgggacg ctcatcgtcg ggatgtgctg gagatcaaga
     4021 ccatgctggc ggggacggac gcgccggagc aggagcacat ggtgtcggcg gaggagctga
     4081 acctcctgat gagcgctccg gacgacgagg aaagctaggc cattcggctc tacctggctc
     4141 tttgcgccct tgtcctccga cggcacacgg ggttgccccc cttgaccttg ccggacaacc
     4201 ggattactgt agaaaaaaac aatacgctgc cggttggaag gcgcgatctg caatgagtcg
     4261 agaggttgca acggatgtat gctcttgttg atatcctggg caagcagtac aaggtggagc
     4321 agggcgccct gctgaaagtc gaccaccttc cccaagacgc gggaagcgcg gtggagttct
     4381 cctccgtgct cctgatctcg gatggcgcct ccacgaagat cgggagcccg tacgtctcgg
     4441 gcgtctccgt ccgtgccgtg gttgagtccc atggacggga ccgcaagctg gtcttcggca
     4501 agtacaagaa gaagaagaac taccggcggc gctacgggca tcgccaggaa tactcgctca
     4561 tcaaagtcga ggggtttgcc ggggtcaact gatccgcggt catcgccccc ggcgatatcg
     4621 gtgactccat gccgaagatg agcgcaaccc tccgcataca cgtttctctt tctccggagg
     4681 ggcttctgcg cgggttcgag gcgcgggggc acgccgggcg ggctcccgcc ggggaaaacc
     4741 tggcgtgcgc cgcggctacg gtcctgctgc ggtctgcggc gcgcgcctgc gccgcgcggg
     4801 gcctggccac cggtggcggg gccggggagc cgggggaact gacgctgagc ctggcgcgcg
     4861 cgtcggagcc cgaaccgggg tggcttcgag gcgtgacgga catgctcctt cgcggggtgg
     4921 acgacctcca gcgggaccac ccgggagcca ttgaggtgga gctggagggc gtagagccat
     4981 agg
//
LOCUS       DASRZS010000441         2790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4470727, whole genome shotgun sequence.
ACCESSION   DASRZS010000441 DASRZS010000000
VERSION     DASRZS010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4470727"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFH83_10845"
     CDS             complement(<1..199)
                     /locus_tag="VFH83_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486908.1"
                     /translation="MTSLSCRITGGDFDSAGLATGKLKEHLARLGVDAAVMRRAMIAA
                     YEAEMNVVIHASTGTLWARVDA"
     gene            complement(196..549)
                     /locus_tag="VFH83_10850"
     CDS             complement(196..549)
                     /locus_tag="VFH83_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486909.1"
                     /translation="MKLTELSNQIDARLHPEAGHRDAQVAYVYAGTTMSDLIGHAATD
                     VLLITSLNNAQLARIGELMDVPGLCLTDGTEPCADLLRLARDADMPILVAAAGLDATR
                     RAAEACLAGAREART"
     gene            complement(829..1929)
                     /locus_tag="VFH83_10855"
     CDS             complement(829..1929)
                     /locus_tag="VFH83_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486910.1"
                     /translation="MHGRGDQESYYVASQWRLMGRRFRRHKLAMIGTVMLVILYLLGL
                     FCEFFAPYGPYERHAAFLFCPPQRVHLVGPEGRLTLRPFLYGLDRKVNLDTMRKTYVE
                     DPATTYPLRFFAKGTPYLLFGFIPASRHFVTAGTGPLFLFGTDDLGRDMLSRVLYAAR
                     ISLSIGLVGVFLSFVLGCLIGGISGFYGRTTDMVIQRIIDFLISLPTIPLWMALSAAL
                     PPKWPSIKVYFGITIILAFLGWCGLARVVRGKILELREEDFVMAARISGAGEPFIIVR
                     HLLPSFISYLIVNLTLAIPSMILGETALSFLGLGLRPPVVSWGVLLQQAQNIRTIAGQ
                     PWLLIPALFVIATVLAFNFLGDGLRDAADPYK"
     gene            complement(1935..>2790)
                     /locus_tag="VFH83_10860"
     CDS             complement(1935..>2790)
                     /locus_tag="VFH83_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486911.1"
                     /translation="QKVTDEQVAALRRQYGLDLPKYQQYFHWISRLLQGDMGRSFAWN
                     KPVSKLIAERLPLTIVISLITLLVTYLVAIPIGIYSATHQYSVGDYAFTVIGFAGLAI
                     PNFLLALILMFLLYKYFGINIGGLFSSQYIDAPWSLAKTRDMLLHLPIPILVIGATGT
                     AYLIRVMRGCLLDELKKQYVVTATAKGLSASRVIFKYPVRIALNPIVSTIGWSLPGIV
                     SGEVITSIVLNLPTTGPLLFEALMGQDMYLAGSTVMFLAALTVVGTFLSDLLLLALDP
                     RIRYEKGA"
BASE COUNT          556 a          841 c          927 g          466 t
ORIGIN      
        1 ccgcatccac cctcgcccac aacgtccccg tggacgcgtg gatcaccacg ttcatttccg
       61 cctcatacgc ggcgatcatc gcgcgccgca tgaccgcggc atccacaccc agtcgggcca
      121 ggtgctcctt gagcttcccc gtcgcgaggc cggcggaatc gaaatccccg cccgtgatcc
      181 tgcagctcag ggaggtcatg tgcgggcctc ccgggcgccg gccaggcatg cttccgcagc
      241 tcgacgcgtg gcatcgagac ccgcggcagc gaccaggatg ggcatgtccg catcccgcgc
      301 cagccgcagg aggtccgcgc atggctccgt gccatcggtc agacagaggc ccggcacgtc
      361 catgagctcg ccaatccgtg cgagctgggc gttgttcagc gaggtgatca ggaggacatc
      421 ggtggccgca tggccgatca gatcgctcat ggtcgtccct gcataaacgt aggcaacctg
      481 ggcgtccctg tggccggctt cgggatgaag ccgggcgtcg atctggttgg acagctcggt
      541 aagcttcata ggcttcctgc cagtgctcgc ccgataagca gcaaacgccg tgccaatcgt
      601 gaatcaatga tcgatagagt ccagacgctt cgtggattca gtggggattt ggcctaccgg
      661 cgattattcc ttgccgggga tgcagttggg gacacagcgg tcaggcgctg ccggtcagct
      721 cggtcggcgg atcgttccgc tccctaggct ctaggcgtcg tcctggcacc gctgcgtggg
      781 cataagtctc ccatgggaga ccggcgcaca ttccctaaca ggaagtgttc acttgtacgg
      841 atctgcggcg tcacggagcc cgtctcccag gaagttgaac gccaggacgg tcgcgatcac
      901 gaagagcgcc gggatcagga gccagggttg gccggcgatc gtccggatgt tctgagcctg
      961 ctggagcagg acgccccagc tgaccacggg gggcctcagc cccagaccca ggaagctcag
     1021 ggcggtttcc cccaggatca tgctcgggat ggcgagggtc agattcacga tcaggtagct
     1081 gatgaacgac gggaggaggt gccgaacgat gataaagggc tcacccgcgc cggagatgcg
     1141 tgccgccatg acgaagtcct cttcccgcag ctccaggatc ttcccccgga ccacccgcgc
     1201 caggccgcac caccctagaa acgcgaggat gatggtgatg ccgaaataga ccttgatcga
     1261 cggccacttg ggtgggaggg ccgcgctcaa ggccatccac agggggatgg tgggcaggga
     1321 gatgagaaag tcgatgatcc gctggattac catgtcggtc gtgcggccat agaaaccgga
     1381 gatgcctcct atgagacacc caaggacgaa actgagaaac acgccgacca gtccgatgga
     1441 caaggagatc cgggcggcat agaggacgcg ggagagcatg tcccggccca ggtcatcggt
     1501 cccgaagagg aagagtggac ccgtcccggc ggtcacgaag tgccgggagg cggggatgaa
     1561 accgaacagg aggtaggggg tgcccttggc gaagaagcgc agcgggtagg tggtcgcggg
     1621 gtcctcgacg taggtcttcc gcatcgtatc gaggttgacc ttgcgatcca gtccatacaa
     1681 gaaaggtcga agcgtgagtc tgccttctgg accaacgagg tgcaccctct ggggcgggca
     1741 gaacaggaac gcggcgtgac gctcgtaggg tccgtagggg gcgaagaact cgcagaacag
     1801 ccccaggagg tagaggatca cgagcatcac ggtcccgatc atcgccagct tgtgccttcg
     1861 gaacctccgt cccatgaggc gccactggct ggccacatag tagctctcct gatcaccacg
     1921 tccgtgcatc gggctcacgc ccccttctca tagcggatcc gcgggtccag cgccaggagc
     1981 agcaggtccg agagaaaggt cccgacgacg gtcagcgccg ccaggaacat gaccgtgctt
     2041 cccgccaggt acatgtcctg acccatgagc gcctcgaaca ggagcggccc cgtggtcggc
     2101 aggttcagga cgatggaggt gatgacctct cccgacacga tccctggaag gctccacccg
     2161 atggtgctga cgatcggatt gagcgcgatg cgcaccggat acttgaagat gacgcgggaa
     2221 gcgctgagcc ccttggccgt ggcggtgacg acatattgct tcttcagctc gtccaggagg
     2281 catccccgca tgacccggat gaggtacgcg gtgccggtgg ccccgatcac gaggatcggg
     2341 atgggcagat ggagcagcat gtcgcgcgtc tttgccaggc tccatggcgc gtcgatgtac
     2401 tggctggaga agagaccccc gatgttgata ccaaagtact tatagaggag aaacatcagg
     2461 atgagggcca gcaggaagtt ggggatggcc agtcccgcga acccgatgac ggtgaaggcg
     2521 tagtccccca cggagtactg atgggtggct gagtagatcc cgatcggtat ggcgaccagg
     2581 taggtgacca ggagcgtgat cagggagatg acgatggtga ggggaagccg ctcggcaatg
     2641 agcttgctca ccggcttgtt ccaggcaaag gaccgtccca tgtccccctg caggagccgg
     2701 ctgatccagt ggaagtactg ctggtacttc ggcagatcca gaccgtactg cctgcgcagc
     2761 gcggccacct gctcatccgt caccttctgc
//
LOCUS       DASRZS010000442         7619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_237081, whole genome shotgun sequence.
ACCESSION   DASRZS010000442 DASRZS010000000
VERSION     DASRZS010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237081"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFH83_10865"
     CDS             complement(<1..504)
                     /locus_tag="VFH83_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486912.1"
                     /translation="MFGKVVLFAFAASLFLCISVSLSAQTASQFPLGNAWVGTAGSGS
                     LGDRDETVVLFFEIPDTTASTLYFAVRNPGNSGNAAQFDEAGSGGTSYWYLIGGTGAL
                     SDPNSQLINYTPLSVTPRAGNLLDSFTRTNQNEGWVYFQGVAPSQGEHIGNKYYFKIV
                     LQVDANVG"
     gene            complement(967..3666)
                     /locus_tag="VFH83_10870"
     CDS             complement(967..3666)
                     /locus_tag="VFH83_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HET6486913.1"
                     /translation="MEAQRQVFLVSHTHWDREWYLTREKSRFLLLGLMEELFTILREQ
                     PDYTFMLDGQTIPLEDYLAMMPDRRAELAGHVRSGRVSIGPWYVLPDELLVSGESHIR
                     NYLVGRRLCDELGGNMNLGYLPDSFGHPSQMPQILAGLGMKEIVFWRGLGPEITRTEL
                     LWEGRDGTEILGVNTPFSYGIAACMPEEPQAFARRLRLKIGLVEPLTDGNVLLLMNGV
                     DHVAPQGTFLPNLKKAGAGFPGYELHHATLKEYLSAVRAQPAPRERARGELRSGYRAY
                     LLGGTISTRMYLKQANHRAQILLEKHAEPLSTLAWLVNGSRYPEQELHHAWKLLLSNH
                     PHDSICGCGIDAIHEEMMQRYRWLGDLTQSIVDRACASIGRAMAGVDAGSNGAGVPAG
                     WDGSFTVWNPLERPRTDVVRVSLGLNERLLRKVNFETGALDESVPQPAGRQPTGVVVR
                     GSDGREIEGVLRGVREEDSMRLSLSTQPEMYRVSRVEIEFVSEALPSVGVAAYDLRLT
                     YGEPQRAADATSIENDLFRVSFEQGTGSLAVQDLRTGIPYPGLHVFEDSGDAGDEYTY
                     SWPARDLRCTLAPGSARVRVGGTRRFPVLVMTATLRVPARLAEDRQCRSAQMTEIGIE
                     STVSLYPGVPRIDIVTEIENTAEDHRLRVLFPLGAPAAVASAEGIFSVDDRPTTPADR
                     TAFADWVEPPSTNPQKSFVSVHAGERGLAVANRGLPEHEVFPDPKGDAVIALTLLRSV
                     GWLSRPDLLARKGNGGWTLPTPQAQCLGRHRFEYSIIPHRGSWEEGGVMGIAHAFEAP
                     ALAFANTHAAAGCRTLSLVAVDREEIVLSAVKRAESGDACLLRFWNASGRTVRARLTF
                     SRAPLAVYRTDLAERRLGRLPASGNRCEVEFGPWKVVTLEAQL"
     gene            3760..6648
                     /locus_tag="VFH83_10875"
     CDS             3760..6648
                     /locus_tag="VFH83_10875"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET6486914.1"
                     /translation="MLRCSLAGDRSLHLRCADGSGEVLRAGPLVRWAPLAGERMSTDL
                     EEPRVLAAPRAGGGGDPQWTLALGPAGLECDVVQQGEGSYGETVFRVTVRNTGAEERR
                     GILLPAFTEWIGAAQSDRSDERFFCMVHDHLYWGQTEPLYAWPPREFAKDVLVTIPAT
                     PTFPPGSAPSCLTAVLAHRSAVEPCLLARGGLRLPPGRAADLTLHLDLVNGSRSEALQ
                     EIWRRRGGYRVSPAAYDFSHYLDPQTAWVRDIVVTWLNWAWDREVMEPHTGAYRLLES
                     LKSARDRFGGYDVFQVWPFWPRAGFDDRFQFDHFRDMPGGIEGLREQVRGAQRMGTRV
                     VASYCVWSESDRDPSPEGLGRSFRDLVSLALDLEADGVLMDIMSAAPKELLEMAAARG
                     RFLTPFNEGDPGWEESQQNLVGRIHNNLAMPRFSLKRYLLPHHPILRVCEPGNEGKVM
                     HSDIGLSFFQGHGVEINSMFPQDSPSCAPDWELLGKAAGVLRANRACFRSADWEPFAE
                     SAAPTVWVNRWPAGGKTLYTLCCTDPRGYRGEVLRLPESGDRSVVDLFRYRPLHCTPE
                     GMVSYELPGFAPDAGMPRGSGDYSFGCIGVFPRRLTVDLELETLSIQVKTPGAGERVE
                     VSAGQVTPAGTPIQRALGSHGRAERERVETERLQLNLYQELGRHTNEAIIVRLLDAED
                     QLLDVQVVPEALVRFFRVERPARSIGRGARDGMVRMPAGSLRFVVRQSQPVWQATYAN
                     QLDVYKPAPEWSREVRLPRFWIDRYPVTNEQYARFVERSGYQPAHPAGFLRHFADGKP
                     PEGQERHPVTNVSYEDAVAYAQWAGCRLPTEEEWQYAAGGGTDRPWPWGDKDPTSELC
                     NLFGGSTTPVDAHPDGSSPLGVEDLVGNVWQWTDSLMDNGRHRVVFLRGGCWHRTPEG
                     VWWVRGGPRPITDHFPLPLFGPAMNRLSTVGFRCVRDDGARDDGALNE"
     gene            6660..>7619
                     /locus_tag="VFH83_10880"
     CDS             6660..>7619
                     /locus_tag="VFH83_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6486915.1"
                     /translation="MSSREKVLAILEHRNTGGPAFWTGNPHPDTVPGYLRRIGGSSLE
                     DLYRYLEDDCRWVMTDPAYRHPQGKRIFDPYLGAPQLSLSQPGCFAECESAAEVDRYP
                     WPDPAYLDFTEVLRQVDRFHDKAVFTGMWCPFFHYVADFFGMDTYFIKMHTNPAVVEA
                     VTDRVVSFLDQANERFFRLLGDRADTFFFGNDFGTQRDLLLSPESFGRFVLPSMKRLI
                     ETAKRFGKKVILHSCGAVYRVIPALIDAGIDGLHPLQAKAQSMDADYLAKTYRGKLAF
                     VGGVDTQDLLIRLSPEQIKTEVRRLKGLLGPNYIVSPSHEAILP"
BASE COUNT         1232 a         2574 c         2560 g         1253 t
ORIGIN      
        1 gccaacgttc gcgtccacct ggagcacgat cttgaagtag tacttgtttc cgatgtgctc
       61 cccctgggaa ggagccaccc cctggaagta cacccatccc tcgttctggt tcgtgcgcgt
      121 gaacgagtcc agcaggttcc cggcgcgcgg cgtcacggac aacggcgtat agttgataag
      181 ctgagagttc ggatcgctca gcgcgccggt tccgccgatc aggtaccaat acgaggtccc
      241 cccggaaccg gcctcgtcga actgggcggc gttgccgctg ttgccggggt ttctgacggc
      301 gaaatacagg gtcgatgccg tggtgtcagg aatctcgaaa aaaagcacga ccgtctcgtc
      361 gcggtccccc agactgccac tacccgcagt gccgacccaa gcgtttccca gcgggaactg
      421 gctcgcggtc tgggcggaca gcgacaccga gatgcataga aacagagatg ccgcaaaggc
      481 gaacaggacg actttcccaa acactcccat agatccttag gcgttccttc cacttcggga
      541 caagagacct gtggtcgtgg tctggggatc gcgactctgc ggaccgatgg ccgcgttgcc
      601 gagtccaagc ccccgccacc cgatgagacg gccctggagc gtcttccgcc tgttcctgtg
      661 cctccgccac tctctgaggg atcgatggtc agccggcaac cacgacggta ctcccgccaa
      721 ccggcagtac atggcatcgc cttgccggtg agaagaatcc ccggcatcga gcacgagcgc
      781 attgctggag gcgcgcggcg gtgcgtaaat cccaaaaaac tcttcgaaaa ctatagcctg
      841 cggagccggg ttattcaggt tggcctgaat aggcgcgcga gggtcactcc ctcgctccgg
      901 aaggactctc cgtccggggt ccccttcggc gaccttcgct cctctggcca agatggcccc
      961 ccgggctcac aactgcgctt ccagcgtaac gaccttccag ggaccgaact ccacctcgca
     1021 ccggttgccg gatgcaggca gacgcccgag gcggcgctcg gcgaggtcgg tccggtagac
     1081 tgccagcggg gcgcggctga acgtaagccg cgcccggacc gtccgccccg acgcgttcca
     1141 gaaccgcagc agacaggcat ctcccgactc ggcccgcttg acggcgctca gcacgatctc
     1201 ctcccggtcc accgcgacca gggaaagggt gcggcatccg gcggccgcgt gggtgttggc
     1261 gaaagccagc gcgggcgcct cgaacgcgtg tgcgatgccc atcacgccgc cctcttccca
     1321 ggagccccgg tgcgggatga tcgagtactc gaaccgatgc cggcccaggc actgcgcctg
     1381 gggggtcggc agggtccagc cgccgttgcc cttgcgagcc aggagatcgg gtcgggacaa
     1441 ccaccccacg ctccgcagca gggtcagcgc gatcacggcg tctcccttcg ggtccgggaa
     1501 gacctcgtgc tccgggaggc cgcggttggc caccgccagg ccgcgctcgc cggcgtggac
     1561 gctcacgaag ctcttctgcg ggttggtgga cggcggctcg acccagtccg caaaggcggt
     1621 ccggtccgcc ggggtcgtcg gccggtcgtc caccgagaag atgccctccg cgctggcgac
     1681 cgcggccgga gcgccgagcg gaaagaggac tcgaagccga tggtcctcgg ccgtgttctc
     1741 gatctcggtc acgatgtcga tccgcgggac cccggggtag agcgagacgg tggactcgat
     1801 cccgatctcc gtcatctgag cgcttcggca ctggcggtct tcggccagtc gggcgggcac
     1861 ccgaagggtc gccgtcatca ccagcaccgg gaatcgccgg gtcccaccca cgcgcacgcg
     1921 cgcgctcccc ggcgccaggg tgcaccgcag gtccctggcg ggccatgagt acgtgtactc
     1981 gtctccggca tcgccggaat cctcgaaaac gtgcaggccc gggtacggga tgccggtccg
     2041 caggtcctgg acggccaggg aaccggtccc ctgctcaaag gagactcgga aaagatcgtt
     2101 ctcgatcgag gtggcgtcgg cggcccgttg gggctcgcca tacgtgagtc gaaggtcgta
     2161 ggcggccact ccgacggagg gaagggcctc ggacacgaac tcgatctcca cccggctcac
     2221 ccggtacatc tctggctgag tcgacaggct cagccgcatc gagtcttcct cccgcacgcc
     2281 gcgaaggacc ccctcgatct cacgaccgtc actgcctcga accacgacgc cggtgggttg
     2341 ccggcccgcc ggctggggaa cggactcgtc cagggcgccc gtctcgaagt tcaccttgcg
     2401 caggagccgc tcgttcaggc ccaggctcac ccgcaccacg tccgtccgcg ggcgctccag
     2461 tgggttccag accgtgaaac tgccgtccca gccggccggt acgccggccc cgttggaccc
     2521 ggcgtcaacc cccgccatcg cccgtccgat ggacgcgcag gcccggtcca caatgctctg
     2581 ggtgagatcg cccagccagc ggtaccgctg catcatttcc tcgtggatgg cgtcgatccc
     2641 gcagccgcag atggaatcat gcggatggtt cgagaggagc agcttccatg cgtgatgcag
     2701 ctcctgctcg ggatagcggg acccgttcac gagccacgcc agggtggaga ggggctcggc
     2761 gtgcttttcg agcaggatct gcgcgcgatg gttggcctgc ttcaggtaca tccgcgtgga
     2821 gatggtcccg cccaggagat aggcgcggta gccggaacgc agctctccgc gcgcccgctc
     2881 tcggggcgcc ggttgagcgc gtaccgcgct cagatactcc ttgagggtgg catggtgaag
     2941 ctcgtagccg gggaagccgg cgcccgcctt cttgaggttg ggaaggaacg tgccctgggg
     3001 cgccacgtgg tcgacaccat tcatcaggag caggacgttc ccgtcggtga gcggctcgac
     3061 aaggccgatc ttcaggcgca gccgtcgggc gaacgcctgc ggctcctcgg gcatgcaggc
     3121 agcgatgcca tagctgaagg gcgtgttcac gcccaggatt tccgtaccgt cccgtccctc
     3181 ccagagaagc tccgtgcgcg tgatctccgg acccagacct cgccagaaga cgatctcctt
     3241 catcccaagg cccgccagga tctggggcat ctgggacgga tggccgaagg aatccgggag
     3301 gtaccccagg ttcatgttcc cgcccagctc gtcgcagagg cgccgcccca ccaggtagtt
     3361 gcggatgtgg gactcaccgg agaccagcag ctcgtccggc agcacgtacc agggaccgat
     3421 ggagacacgg ccgcttcgca catggccggc cagctccgcg cgccggtccg gcatcatggc
     3481 caggtagtcc tccagcggga tggtctgccc atcgagcatg aacgtgtagt ccggctgctc
     3541 tcgaaggatc gtgaagagct cctccatgag gcccaggagt aggaaccgac tcttctcccg
     3601 cgtcagatac cactcccggt cccagtgcgt gtgcgaaacc agaaagacct gcctctgcgc
     3661 ttccatcggc tcccccgtcg cgggattcta ccggccgcat ccgcctcttg gcaatggcgg
     3721 tggaccggga gcaccctgtg cgcaaaggag tctggcagga tgctgaggtg ttccctggcg
     3781 ggcgaccggt ctctgcacct gaggtgcgcg gatggttcag gggaggtatt gagggccggc
     3841 ccgctggtgc gatgggcccc gctcgctggg gagcggatgt cgacggacct cgaggagcca
     3901 agggtgctgg ccgcgccgcg ggcgggtggc ggcggggatc cccagtggac gctggccctt
     3961 ggcccggcag gcctcgagtg cgacgtagtc cagcagggcg agggcagcta cggtgagacg
     4021 gtcttcaggg tgacggttcg caacaccggc gcggaggagc gtcgaggcat cctgctgcct
     4081 gcctttaccg agtggattgg agcggcgcag tcggaccgct cggatgagcg cttcttctgc
     4141 atggtccatg accacctgta ctggggtcag acggaaccac tgtacgcctg gccgccgcgg
     4201 gagttcgcca aggatgtgct cgtcacgatc ccggccacgc ccacgttccc gccgggcagc
     4261 gcgccgtcct gcctcacggc ggtcctggcg caccggtcgg ccgtggagcc gtgcctcctg
     4321 gcgcgcggcg gtcttcgact tccgcctggc cgcgccgcgg acctgaccct gcacctggac
     4381 ctggtaaacg gctcgcgctc cgaggctctg caggagatct ggagaaggcg gggcggttac
     4441 cgcgtgtccc cggccgccta cgacttttcc cactacctcg atccgcagac cgcctgggtg
     4501 cgagacatcg tggtcacgtg gctgaactgg gcctgggatc gggaggtgat ggagccgcac
     4561 acgggggcgt accggctcct ggagtccctg aagagcgcca gagaccggtt cggcggatac
     4621 gacgtgttcc aggtgtggcc cttctggccc cgggccggct tcgacgaccg gttccagttc
     4681 gaccatttcc gcgacatgcc cggtgggatc gaggggctgc gcgagcaggt ccgcggcgcc
     4741 cagcggatgg gcacgcgcgt ggtcgcttcc tactgcgtgt ggagcgagag cgatcgggat
     4801 ccctcccccg agggtctggg tcgatcgttc cgagacctcg tctccctggc gctcgacctg
     4861 gaggcggatg gcgtcctcat ggacatcatg agcgccgcgc ccaaggagct tctcgagatg
     4921 gccgccgcgc gaggtcggtt cctgaccccg ttcaacgaag gggatcccgg ttgggaagag
     4981 agccagcaga acctggtggg ccgcatccac aacaacctcg ccatgccccg attcagcctg
     5041 aagcgttacc tccttcctca tcacccgatc ctgcgcgtct gcgagccggg caacgagggc
     5101 aaggtgatgc acagcgacat cggcctgtcc ttcttccagg ggcacggtgt ggagatcaac
     5161 tccatgttcc cccaggacag cccgagctgc gcgccggact gggagctcct gggaaaggct
     5221 gccggagtcc tgcgcgccaa ccgcgcctgc tttcggtccg ccgactggga gccgttcgcg
     5281 gagagcgccg cgccgaccgt ctgggtcaac cgatggcccg ccggcgggaa aaccctgtac
     5341 accctttgct gcaccgatcc acgcggttat cggggagagg tgctccggct cccggagagc
     5401 ggggaccgct cggtggtgga cctgttccgc taccgcccct tgcattgcac tccggaaggc
     5461 atggtttcgt acgagcttcc cggctttgcg cccgacgctg gaatgccccg cggctctggc
     5521 gattattcct tcggctgcat cggcgtcttc ccgaggcgac tgaccgtgga cctcgagctg
     5581 gagacgctga gcatccaggt caagacgcct ggggccggcg aacgggtcga ggtatccgcg
     5641 gggcaggtga cgccggccgg gacgcccatc cagcgggcac tcgggagcca cggccgcgcg
     5701 gagcgtgagc gcgtggagac cgagcgcctc cagctcaacc tctatcagga gctgggacgt
     5761 cacaccaacg aggcgatcat cgtccgactc ctggacgcgg aagaccagct cctggacgtg
     5821 caggttgtgc cggaagccct ggtccgcttc ttccgcgtgg agaggccggc gcggtccatc
     5881 ggccgaggcg cacgggacgg gatggtgcgc atgcccgccg gctccttgcg cttcgtggtg
     5941 cgtcagagcc agccggtgtg gcaggccacg tatgcgaacc agcttgacgt ctacaagcct
     6001 gcgccggagt ggtctcggga ggttcggctc ccaaggttct ggatcgaccg gtaccccgtg
     6061 accaacgagc agtacgcgcg gttcgtcgag aggtccggct accagcctgc tcatcccgcg
     6121 gggttcctcc ggcactttgc ggacggcaag ccccctgagg gacaggaaag gcatccggtg
     6181 accaacgtga gctacgagga cgcggttgcc tatgcgcagt gggccggttg ccgcctgccc
     6241 acggaggagg agtggcagta tgccgcgggc ggcgggacgg acaggccgtg gccgtgggga
     6301 gacaaggatc ccacctcgga gctctgcaat ctcttcggcg gctcgacgac accggtagac
     6361 gcgcacccag acggttccag cccgctgggc gtcgaggatc tggtcgggaa cgtctggcag
     6421 tggaccgaca gcctgatgga caacggccgc caccgggtgg tgttccttcg aggcgggtgc
     6481 tggcaccgca caccggaagg cgtatggtgg gtccgcggtg ggccgcgtcc catcacggac
     6541 cacttcccgc tgccgctctt cggcccggct atgaaccggc tttccaccgt gggcttccgc
     6601 tgtgtgaggg acgacggcgc gcgggacgac ggcgcgctga acgagtagag aggagtctga
     6661 tgagctctcg agagaaggtc ctggcgatcc tggagcatcg gaacaccggc ggcccggcgt
     6721 tctggaccgg caacccccac ccggacacgg tccccgggta cctccgccgg atcggcggct
     6781 cctccctgga ggacctgtac cggtacctcg aggacgactg ccgctgggtg atgaccgatc
     6841 ccgcctaccg tcacccccag ggcaagcgga tcttcgatcc ctacctgggc gcgccgcagc
     6901 tcagcctgtc ccagcccggg tgcttcgccg agtgcgagtc cgcggccgaa gtggaccggt
     6961 atccgtggcc ggacccggcc tacctcgact tcaccgaagt gctgcggcag gtggaccggt
     7021 tccacgacaa ggccgtgttc accggcatgt ggtgcccgtt cttccattac gtcgccgact
     7081 tcttcggcat ggacacctac ttcatcaaga tgcacacgaa ccccgcggtg gtggaggcgg
     7141 tcaccgaccg cgtggtctct tttctggacc aggcgaacga gcggttcttc cggctcctcg
     7201 gcgatcgcgc ggacacgttc ttcttcggga acgacttcgg aacgcagcgg gatctcctgc
     7261 tctctcccga gtccttcgga cggttcgtgc ttccgtccat gaagcggctc atcgaaaccg
     7321 ccaagcggtt cgggaagaag gtcatcctcc attcctgcgg cgccgtgtac cgggtgatcc
     7381 cggcgctgat cgatgccggt atcgacggtc tgcatcccct gcaggcaaag gcacagtcga
     7441 tggacgcgga ctacctggcg aagacctacc gcgggaagct ggcgttcgtc ggaggcgtgg
     7501 acacccagga cctcctgatc cgcctgagcc cggagcagat caagaccgaa gtccgaaggc
     7561 tcaaggggct gctcggcccc aactacatcg tgagcccgag ccacgaggcc atcctcccc
//
LOCUS       DASRZS010000443         4778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4088678, whole genome shotgun sequence.
ACCESSION   DASRZS010000443 DASRZS010000000
VERSION     DASRZS010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4088678"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VFH83_10885"
     CDS             <1..637
                     /locus_tag="VFH83_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486916.1"
                     /translation="YTLGEVKVDVLNDITLDIGRGEFLTIMGPSGSGKSTLLYLMGAL
                     DAPTSGTLTVNGRDLATLRDPEQSRLRRRDIGFVFQFYNLVPNLSLEDNVILPVLLDG
                     KNLHQYRRRLDEILDLVGLADRRRHTPRELSGGQQQRAAIARALVNEPEIVLADEPTG
                     NLDSHSGTEIMALLKHINETRGKTIVQVTHSQEAAGYGHRTIHLRDGRIWE"
     gene            634..1695
                     /locus_tag="VFH83_10890"
     CDS             634..1695
                     /locus_tag="VFH83_10890"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET6486917.1"
                     /translation="MRKGRIGVPLLAVCMAVLLAASGCAKGGANAGSAPAAAASTTTT
                     AKGATNASGKAGGATGGDPAAESKHVEAFGIVKADTVHALSLEFPAVLQKRLVSEGQR
                     VHRGDALFRFSRRDYDAQVTDKGYELTLARLDLSKAERDLAKLREDLAYAREDADKAQ
                     KDASDKERLLSLGAASRRDVEDAQRTLSARQKSVRELERAVAEYDSDVNGLEAQKTHI
                     AILEEELARLTDLAHRPFITNDVVVCDLREAVVSDIGYVEGDAIARDRKLCNLIDLDS
                     VIVEANVPEEFYKDVKVGSPASVVPVADTTRKYEGKVTRISNLATLHNGETVVPVQIT
                     LAAPDAFLLPNFNVDVSIY"
     gene            1756..2208
                     /locus_tag="VFH83_10895"
     CDS             1756..2208
                     /locus_tag="VFH83_10895"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6486918.1"
                     /translation="MDKIGTTTIMQICLVVKDAEATMRHFMDLFGIEKGTLKQIPPAS
                     KSKTFYRGKEIDTTTKYYVFRMGDIVFELTQPDDSPSVWKEVLDQKGEGLCYLGLMVE
                     DSAKVVSFLEQKGNPRIHHGGTPESNYSIVDTRDSLGVLLNVKMQKQS"
     gene            2277..2687
                     /locus_tag="VFH83_10900"
     CDS             2277..2687
                     /locus_tag="VFH83_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6486919.1"
                     /translation="MAVTGLGHLALNVSDMTRSAEFYTRVLGFQHLFELKDDKGNPWI
                     SYFKVGPGQFIELFYPRGEGPAATKAIVGFSHMCFVVDDIKRTEAEILRGGGTLDRPI
                     KLGADGNYQMWVRDPDGNRIELMQIMPGSPQAKS"
     gene            2809..4032
                     /locus_tag="VFH83_10905"
     CDS             2809..4032
                     /locus_tag="VFH83_10905"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6486920.1"
                     /translation="MKRLAIMLLMALGSVLGAYAASNAITVWLGSWWQAAVPDIQARF
                     ARDNPGYSVKIQLQPVNNYLENATTAILGGTPPDALDLDTLMIPTPIGKNLLSPLDDF
                     MKRYNIKKSDFPEAVFNSGVAGGQTYAIPDRSAVTVMFYNKTLFDKAGVPYPAADVSW
                     DDFATLAKKLTIPGKQYGWGIAATNADPANVMTSFCPILFSFGGTFLNADMTRSQLGS
                     PAGIKAIRYYSDLYRTYKVVPEGTVNYTITRDLIPMLQGGTLAMCPMNDSNLPPTIAV
                     FDKNGWKWGTAPLPGGGLSRAGGWSMTIPATSQNVAAAEKFIAWFIRPDVEAQQNIVM
                     PGTLSAQGQGLWKNPIYDIFWQVAKIARSVPPTPHWTEIQNLVVIDLQKALQGTMTPE
                     AVGADLDTQINALLQ"
     gene            4119..>4778
                     /locus_tag="VFH83_10910"
     CDS             4119..>4778
                     /locus_tag="VFH83_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6486921.1"
                     /translation="MSTPRVRAILYRLTPYSLVLPCAVVLIVMMIYPIVQILQFSFSK
                     VTLPAFKTTFVGLANFLRVTRKAEFGSVALHTLIWTVVSLVLRFALGMGAALLVDSVP
                     RGMRVVRVLGMLPWIMPSIVAANIWRWIYSTDYGFLNYALRLFFPGATVNWLGDARLA
                     LGSVVFAFSWLGFPFVMLMLLAGLQGIPVEHKEAARVEGANGLQVFRHITIPFLRPVI
                     LT"
BASE COUNT          885 a         1564 c         1561 g          768 t
ORIGIN      
        1 ctataccctc ggcgaggtga aggtggacgt cctgaacgac atcacgctgg acatcggccg
       61 cggggagttt ctcaccatca tgggtccctc cggatccggc aagagcacgc tcctgtacct
      121 gatgggcgcc ctggacgcgc ccacctcggg taccctcacc gtgaacgggc gggatctcgc
      181 caccctgcgg gaccccgagc agagccgtct ccgtcggcgg gatatcgggt tcgtgttcca
      241 gttctacaac ctggtgccga acctcagcct ggaagacaac gtcatcctgc ccgtgcttct
      301 ggatgggaaa aacctgcacc agtaccgccg ccgcctcgac gagatcctgg acctggtcgg
      361 actggcggac cgacggcggc acacgccgcg ggagctctcc ggcggccagc agcagagagc
      421 ggccattgcg cgcgccctgg tgaacgagcc cgagatcgtg ctggcggacg agccgacggg
      481 caacctggac agccacagcg gcaccgagat catggcgctt ctcaagcaca tcaacgagac
      541 ccggggaaag accatcgtgc aggtgacgca ctcgcaggag gccgcgggct acgggcatcg
      601 caccatccac ttgcgggacg ggaggatctg ggaatgagga agggaaggat cggggtgccg
      661 ctcctggccg tctgcatggc ggtgctgctg gccgcgagcg gctgcgcgaa gggcggggcg
      721 aacgccggca gcgcgccggc agcggcggcc tccacgacga ccacggcaaa aggcgcgacg
      781 aacgcgtccg ggaaggccgg tggcgccacg ggcggcgatc cggcggcgga atcgaagcac
      841 gtggaagctt tcggcatcgt caaggcggac acggtgcacg ccctctccct ggagttcccg
      901 gcggttctcc agaagcgcct cgtctccgag ggccagcggg tccatcgcgg cgatgcgctc
      961 ttccgtttct cccgccggga ctacgacgcg caggtgaccg acaagggcta cgagctcacc
     1021 ctggcgcgac tggacctgtc gaaggccgag cgtgacctcg ccaagctccg ggaagacctc
     1081 gcgtacgcac gggaggacgc cgacaaggcg cagaaggacg cctccgacaa ggagcgcctg
     1141 ctctccctgg gcgccgcatc gcgtcgggac gtggaggatg cccagcggac gctgagcgcc
     1201 cgacagaagt ccgtgcgtga gctcgagcgc gccgttgcgg aatacgattc cgacgtgaac
     1261 ggcctggagg cgcagaagac ccacatcgcc atcctggagg aggagttggc gcggcttacg
     1321 gacctcgccc atcgcccctt catcaccaat gacgtcgtgg tctgcgacct tcgggaggcc
     1381 gtggtcagcg acatcggcta cgtggagggg gacgccattg cccgcgaccg caagctctgc
     1441 aacctcatcg acctggacag tgtcatcgtg gaggcgaacg tccccgagga gttctacaag
     1501 gacgtcaagg tagggagccc cgcatccgtg gtgccggtgg cggacacgac gcggaagtac
     1561 gaagggaagg tgacccggat ctccaacctg gccacgctgc acaacggtga gacggtggtg
     1621 cccgtgcaga tcacccttgc cgcgccggat gcgttcctgc tgccgaactt caacgtggac
     1681 gtgagcatct attgacgggt gcctgggtcg cggtgccata ctccttcgcc gccatgcggc
     1741 aaggagagca cggacttgga caagatcggg acgactacca tcatgcagat ctgcctcgtg
     1801 gtgaaggacg cggaggccac gatgcgccac ttcatggacc tgttcgggat cgagaagggg
     1861 accctgaagc agatcccccc ggcgtcgaaa tcgaagacgt tctaccgcgg caaggagatc
     1921 gacaccacga cgaagtacta cgtgttccgg atgggggaca tcgtattcga gctcacgcag
     1981 ccggacgact ctcccagcgt ctggaaagag gtgctggacc agaagggcga aggcctctgc
     2041 tacctgggcc tcatggtgga agactccgcg aaggtggtga gcttcctgga gcagaagggc
     2101 aacccgcgca tccaccacgg gggcacgccg gagtccaact acagcatcgt ggacacccgc
     2161 gactccctcg gggttctcct caacgtaaag atgcagaagc agagctgacg cgcggaatgg
     2221 ggcgaccgaa gggagccctg acgcgcggca ttcgcctgga cacaggaggt aggcacatgg
     2281 cggtcactgg actgggtcat ctggccctga acgtctcgga catgacgcgg tccgcggagt
     2341 tctatacgcg cgttctgggg ttccagcacc tcttcgagct gaaggatgac aagggcaatc
     2401 cctggatcag ctacttcaag gtcggtccgg gacagttcat cgagctgttc tacccccgcg
     2461 gagaagggcc ggccgccacc aaggccatcg tggggttctc ccacatgtgc ttcgtggtcg
     2521 acgacatcaa gaggacggaa gccgagatcc tgaggggcgg cgggaccctg gatcggccca
     2581 tcaagctggg ggccgacggc aactaccaga tgtgggtccg ggaccctgac ggcaaccgga
     2641 tcgagctgat gcagatcatg ccgggttcgc cccaggcgaa gagctgatcc gcgccggggc
     2701 gaccgcccgc ccggcgaggc tccgagccga ccgaccagcg tttgacagga acccagggcc
     2761 gtagtaccta gagggtgcac ggtagaaacc ctgaaggagg gaatcggtat gaagagactc
     2821 gccatcatgc tgctcatggc cctgggttcg gtcctcggag cctacgcggc cagcaacgcc
     2881 attaccgtct ggctggggag ctggtggcag gccgccgtcc cggacatcca ggcgcggttt
     2941 gcccgggaca accccggcta ttcggtcaag atccagctcc agccggtgaa caactacctg
     3001 gagaacgcga ccaccgcgat cctcggcggg acgcctccgg acgcgttgga cctggacacg
     3061 ctcatgatcc cgacgcccat tggcaagaac ctgctctcac cgctggacga tttcatgaag
     3121 cgctacaaca tcaagaagtc ggacttcccc gaggcggtgt tcaactccgg ggtggcgggc
     3181 ggacagacct atgccattcc cgaccgctcg gcggtcaccg tgatgttcta caacaagacg
     3241 ttgttcgaca aggccggcgt gccctatccc gccgcggacg tgtcgtggga cgacttcgcg
     3301 accctggcca agaagctcac catccccggc aagcagtatg gctggggaat cgcggcgacc
     3361 aacgccgatc cggcgaacgt catgacctcc ttctgcccga tcctgttcag cttcggcggc
     3421 accttcctga acgcggacat gacccgaagc cagcttggat cccccgccgg catcaaggcc
     3481 atccgctact actcggacct gtaccggacg tacaaggtgg tcccggaagg gaccgtgaac
     3541 tacacgatca cccgggacct gatccccatg ctgcagggcg ggaccctggc catgtgcccc
     3601 atgaacgaca gcaacctgcc gcccaccatc gcggtattcg acaagaatgg ctggaaatgg
     3661 gggaccgcgc cgctgccggg cggcgggctc tcccgcgccg gcggatggtc catgaccatc
     3721 cccgccacca gccagaacgt ggcggccgcg gagaagttca tcgcctggtt catccgcccg
     3781 gacgtagagg cccagcagaa catcgtcatg cccggcaccc tgtccgcgca ggggcagggg
     3841 ctctggaaga acccgatcta cgacatcttc tggcaggtgg ccaagatcgc gaggtcggtg
     3901 ccccccacgc ctcactggac cgagatccag aacctcgtgg tcatcgacct ccagaaggcg
     3961 ctccagggga ccatgacgcc ggaggcggtc ggcgcggacc tggacacgca gatcaacgcc
     4021 ctgctccagt agccggcctg gttgagccgt cgtccatcgc cgatgagcgg ggagggcggg
     4081 cgaacgcccg ccctctcccc attcttcggt agcagaacgt gagtacgccc cgcgtccggg
     4141 caatcctgta ccggctgacg ccttattccc tcgtcctccc gtgcgccgtc gtcctgatcg
     4201 ttatgatgat ctaccccatc gtccagatcc tccagttcag cttcagcaag gtgacgctgc
     4261 cggccttcaa gacgacgttc gtcggtctgg cgaacttcct tcgagtcacg cggaaagcgg
     4321 agttcgggtc cgtggcgctc cacaccctca tctggaccgt cgtatcgctg gtccttcggt
     4381 ttgcgctggg gatgggcgcg gcgctcctgg tggactcggt gccccgcgga atgcgcgtgg
     4441 tgcgagtgct cggcatgctt ccgtggatca tgccctcgat cgttgccgcc aacatctgga
     4501 gatggatcta cagcaccgac tacggctttc tgaactacgc cctgcggctc ttcttccccg
     4561 gcgccaccgt caactggctc ggagacgcgc ggctggcctt gggctcggtg gtgttcgcct
     4621 tctcctggct cggcttcccc ttcgtgatgc tgatgctgct ggccgggctg caagggatcc
     4681 cggtggagca caaggaggcc gctcgggtgg aaggcgccaa cggcttgcag gtgtttcgtc
     4741 acatcacgat ccccttcctg cggccggtga tcctgacg
//
LOCUS       DASRZS010000444        12619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_238078, whole genome shotgun sequence.
ACCESSION   DASRZS010000444 DASRZS010000000
VERSION     DASRZS010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 12619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12619
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238078"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..1187
                     /locus_tag="VFH83_10915"
     CDS             267..1187
                     /locus_tag="VFH83_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486922.1"
                     /translation="MVRYILNRVLVAILTMFVLATVTFFLLRLVPGDPCAGPKVTPEV
                     KAKLRAHYGLDKPLMQQYGIYMGHLLQGDFGYSMAKRGNRVNDIIAQAFPASLDLGIR
                     SMIFAIVLGLFLGVIAALNRGKAWDYLTVILVLVGISVPSFVVAAFLQWFFGVQLRIL
                     PVARYDTFKHTLLPTFALSLGTMAALARYMRASMLEVTGADFIKTARAKGLRRFQIVW
                     RHQVRNALFPILTILGPAIAMVLTGSFIIETIFAIPGLGRFYVTAMQNLDYTLVLGLT
                     LFFGFFLIAMNLLVDFAYGLIDPRVRHTRR"
     gene            1200..2117
                     /locus_tag="VFH83_10920"
     CDS             1200..2117
                     /locus_tag="VFH83_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486923.1"
                     /translation="MSEADRGLFTPLDRAERAGLKTQTRTIAFWSDVWRRFRGHRAGM
                     AGLVILGLFVLLAIIGPSLVRYHYDIIDLNETYIYPGAQHWFGTDVLGRDLWARVWIG
                     ARVSLLIGVVSALSQMVIGTIIGSISGIYGGKVDMILMRFVDILIAVPFLIWVSLFML
                     VLNPGITSIILAFTVTEWKGTARLVRGEVLRLKETEYVLATRGMGASTWRLIQRHFFP
                     NVLPVIIVSVTFQVTGAIFGEAFLSYIGLGIQPPVTSWGILISSGISEMRSYPYMLFI
                     PAVFISLTMLSLQLIGDALRDATDPKLRQ"
     gene            2128..3174
                     /locus_tag="VFH83_10925"
     CDS             2128..3174
                     /locus_tag="VFH83_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486924.1"
                     /translation="MGDTATRSAGERLLDVRNLTISFHTFAGEVSAVRGVDFDLKRGE
                     VIAIVGESGSGKSVSTQAILGLTPVPPGEIKSGEILFQGEDLLSYDERRLSEVSGSRI
                     SMIFQDPMTSLNPTMKVGGQIREVLHQHSRMSREESRTRALELMNLVGIPEPSVRLGQ
                     YPHEYSGGMRQRAMIAMAMANRPEILIADEPTTALDVTIQSQIFRLLRGLQKDFHTAI
                     ILITHDLGVVAGMADRIVVMYAGKVMEEGRTDQIYYRPSHPYTAGLLQAVPSLADDAR
                     QKLRTIEGTPPFLLNVPPGCPFAERCEYAMRVCREKEPPYFRPDPSNRVACWLYHEGA
                     AEQRQTFEKRERAR"
     gene            3171..4127
                     /locus_tag="VFH83_10930"
     CDS             3171..4127
                     /locus_tag="VFH83_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6486925.1"
                     /translation="MSGKILELRGLTKHFPMSRGRILKAVEAIDLDVYEGETFGLVGE
                     SGCGKSTLGRTIKGIYEPTAGTIRFQGQDTASMTRPEQKVYRRNLQIIFQDPYSSLNP
                     RMTVRELIGEGLEIHRLGNRERQKRRVFETLEMVGLNPEHVTRFAHEFSGGQRQRLAI
                     ARALAVEPRFIICDEPISALDVSIQGQIVNLMKDLQRKLGLTYLFIAHDLSMVKYISD
                     RVGVMYLGRLVEVAEKNELFRNPQHPYTQALLSSIPIPDPRIERQRKEIPIEGEIPSP
                     MMRIDGCSFRARCPFAAPRCASMEYTLKDIGNQHYVMCTKFP"
     gene            4205..5317
                     /locus_tag="VFH83_10935"
     CDS             4205..5317
                     /locus_tag="VFH83_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET6486926.1"
                     /translation="MKAAVLQELNTIGVRETDRPRCGDDEAILKVHACAVCGSDLRIF
                     HYGNDRVKPPAVIGHEIAGQVVETGKNVTRVHVGDRVAVGADVPCGTCYWCANGMGTN
                     CRINYAIGYQFPGGFQEYMVLNRTILAYGPVTPIADTLAYAEAAIAEPLACAINGLEL
                     AQFGLGKSICIIGLGPIGCMMLELSQVFGASKVFAAQRSPLRMEMARPFLPRARFIAT
                     EQEDLVQTVLRETDGEGVDLVITTAGTVKAHEDAVQMVRHRGYVNLFGGLKNQPKLTI
                     DSNLIHYKECFVMGSHGSLPRHHEAAVGLISRGAVRAKDYLSAVYPLEGIREALDFHE
                     SRKGLKVIVAPHGEEAARARGAGARGRDPGAGGALR"
     gene            5314..6021
                     /gene="rpe"
                     /locus_tag="VFH83_10940"
     CDS             5314..6021
                     /gene="rpe"
                     /locus_tag="VFH83_10940"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET6486927.1"
                     /translation="MSGVKIAPSLMCADFIRLGDELDVMRQEGIEYLHLDIMDGHYVP
                     NLALGPDFAKRVRAHSPIPLDIHLMIEEPDKLIPLFGGIPDCVISIHPEVSYHPLRTL
                     QLIRDMGARPAIAIDPAMPTSAIEELLPHVTLVCVMTVNPGYAGQKLIPEALPKIRQV
                     SEMAERGKLDIEVEVDGNVSWQNVPRMVEAGARVLVAGTSSLYDRTAGLRENIKRLRA
                     VATAGAPPAVAPRRAQG"
     gene            complement(5951..7258)
                     /locus_tag="VFH83_10945"
     CDS             complement(5951..7258)
                     /locus_tag="VFH83_10945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6486928.1"
                     /translation="MRRHGVEIPATLSWGRAVGMFSRNARLYLATAALMGLTIFGGIY
                     ALLFNLYLVRLGYGLSFIGSINATALLGWAAACLLAGSVGRRWGSRGSMTSGLLISMV
                     GYLAVPFCELMRAGIARSVWLIVSYLVANVAISLYDVNSQPFLVESSTREERSHLFSL
                     QAALWPISAFVGSLLGGFLPGLLSGTSASTAPGPYRFSLMASAATLIVAVVVLSRARP
                     VTAETAAPAPTRAETSGSAMLLVLVGVLLLLHGFGDGTAKTFFNVYLDTELHTSTARI
                     GLLAAVAQLVSVPAALLMPTTVRIWGHRRVFQWGTVGIVAGLIPLALVPHWTAAGAGY
                     IVVVCLIALTRAALMVYLMEVVPERQRAPMTGVYIMAIGLSWSVAAAGGSQLIAALGY
                     PMFYLAGAALTAASLAVFARITPGRAEARRPGAPQPSPRPEGV"
     gene            complement(7255..8760)
                     /locus_tag="VFH83_10950"
     CDS             complement(7255..8760)
                     /locus_tag="VFH83_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET6486929.1"
                     /translation="MSLLGVDMGSSSCKAAVFTPAGETLCSASRSYSMTFPGPAMAEM
                     DPRDLWNATVQAVREAAAAAEGRGDPVQALAISAHGETFIPVNATGDPVCPAIMNIDN
                     RAVAEAAWWERTIGKERLYGIVGTMAHPMYSMTKVRWLQLHRPEVVSATAQLLGPGEY
                     VLRRMGLPPLTDFSLACRWLVFDIHTHRWSDELLAAAGVSRDRLPRPVAAGTAAGRLS
                     AAMAREMGVPAGTIVAVGGHDQACCALGAGAIGPGVVSDSAGTYECFHSASPAPCLGP
                     ASFRANLNSYCHVVPDTYVTLAFFPSGIVVRWFLDRLAEAESARAVASGVNPHEWFES
                     RAPADPSGLTVTPHLIGACNPHWNPRATVAVTGLTGDAGIFHLYKGILEGIACEFALN
                     ADVLEEATAPFTRVRVTGGGTGSRLGLRLRAALSGKTVETLRNPEAVCLGAAILAGTA
                     AGVYRDVQDGVAQAVRVAETVEPEPELARSYETQVARYRMLYPALAPVFDR"
     gene            complement(8757..10964)
                     /locus_tag="VFH83_10955"
     CDS             complement(8757..10964)
                     /locus_tag="VFH83_10955"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET6486930.1"
                     /translation="MAIVPTRAFVRSGDSPGMRYCAGAVVLDEAFTASRLCTRYWSAA
                     GQVVPETHAGTIAWQADEPADSFGLAIDGRSLAGGYQWVSASTEPDPSAQGPDRQVPV
                     KASIRLSHPASGVEVTVNTRLDGSPFLVRWLSVANHSARPIGVSAVAPFAGMIWTRRI
                     DEHLPPGVSGPFQLAYTHRFQWGQEGDFWFEPLAPGVTTVNGGKLGRSGWGRPAFWAR
                     DIAIGQTLVCELAWGGNYEFALDCRLLDSPSRARLFFRMGLCGQDEALRVLLPGETVR
                     TPAVHVGLFQADVDRIVQETHDHVRSAVLPVPIPGRHVEIEANHRGYLCDRENEPDLK
                     RDEEVAKAVGAELYVVDAGWYGNDPNEWWNNAGDWRAGSWLPNGLEPIADHAHELGMR
                     FGLWVEIEAAGRNSSLRREHAEWLLCRDGTPVNGRALDFTNPQVVRWAESEVERIIRQ
                     YRLDLFRIDHNHTLTPSGNRRFHGYTEDLTWRYYENLYGMIDRLRSRFPEVVFQNCAG
                     GGGRLDWGTLSRFHNTELSDWMRMPRGLRILNGVTVSLPPEILLRTFGTEVGEHDLDG
                     DLETQLRLVSLCRPILRGIAPSLDELTPALKARIDHALELFRRVIRPILVESRVFHHT
                     PFLPIYGPSPWCVLEYASRDGEHAVAGLFRTSGEGPEEFLLKPRGLRMDGVYRVTFDS
                     LSADTATATQGSVTMSGMELARWGLPVRLPNVMSSELVLFARTEPGAVNQEDA"
     gene            11147..11932
                     /locus_tag="VFH83_10960"
     CDS             11147..11932
                     /locus_tag="VFH83_10960"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET6486931.1"
                     /translation="MRIARVRTANGAARHAVLDGDTAQLIEGDVFSTWKRVGKPFPLN
                     EATLLAPLVPVNILCFGRNYRAHAEEGGASLPTRPLLFLKATSCLNDPEAPIVLPPAA
                     PSQIDFEAELAVVIGMPAYRVSRDQALRHVFGYTCGNDVSARDIQESDGQWSRAKSFD
                     TFGPLGPWIETRLDPGNLRIQGRLNGEVMQDATTAQLVFDVAYLISFLSQGMTLQPGT
                     VLMTGTPAGCGFARKPPVWLAPGDVYEVEIDGIGILRNPVKSA"
     gene            11991..12536
                     /locus_tag="VFH83_10965"
     CDS             11991..12536
                     /locus_tag="VFH83_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486932.1"
                     /translation="MRRICAWCKAPLNREKAGPISHGICEPCSVYVLSEKFRFRDFLE
                     SIEAPLLAVDGEGRAITANTVALKTLGKGMPQVGGRLPGEVMECEYSRLPGGCGKTVH
                     CNGCQIRMSVDYTRSTGEARVRTRAYIRIQESQGVKQYDYYVSTEKLDGVVLLRIDET
                     RPCGQGGGVPAGKVDAEPRAL"
BASE COUNT         2154 a         4188 c         4221 g         2056 t
ORIGIN      
        1 cgctgttctc cgacaacgcg ccgtgcatcc ccctcatgtg gaacaagggc agcgcggcgt
       61 tgagcgctcg ggtgaagggg ttcaccttct cccttaccgg gttgaccacg gattacctct
      121 tcgcggatct cacgaagtag cgcgaacggc atcttgggag gcgccccgtc gcggggcgcc
      181 tccgttgtct ctggtgcgcg gatccctcac tacccgccag aaggagagct ccagaggccg
      241 gcattcggtc tgggaagggg ggaaccatgg tgaggtacat cctgaaccgc gtcctggtcg
      301 cgattctcac gatgttcgtc ctggctacgg tcacgttctt cctcctgcgc ctggttcccg
      361 gggatccgtg tgccgggccc aaggtgaccc cggaggtcaa ggccaagctg cgggcgcact
      421 acggcctgga caagcccctc atgcagcaat acgggatcta catggggcac cttctccagg
      481 gcgacttcgg ctactccatg gcgaagcgcg gcaaccgggt gaatgacatc atcgcccagg
      541 cattcccggc gtcgctggac ctcggcatcc ggtcgatgat cttcgccatt gtgctggggt
      601 tgttcctggg ggtcatcgcc gcgttgaacc gcggcaaggc atgggactac ctcaccgtca
      661 tcctggtcct ggtcgggatc tccgttccca gcttcgtggt ggcggccttt ctgcagtggt
      721 ttttcggcgt gcagcttcgg atcctcccgg ttgcgcgcta cgacacgttc aagcacaccc
      781 tgcttcccac cttcgcgctg agcctgggca ccatggcggc gctggcccga tacatgcggg
      841 ccagcatgct cgaggtgacc ggggcggact tcatcaagac ggcacgcgcc aagggcctgc
      901 gccgcttcca gatcgtctgg cgccaccagg tccgaaacgc gctcttcccg atcctcacga
      961 tcctgggccc ggccatcgcc atggtgctga cggggtcttt catcatcgag acgatcttcg
     1021 ccatcccggg gctgggcagg ttctatgtca cggccatgca gaacctggat tacaccctcg
     1081 tcctggggct gacgttgttc ttcggattct tcctgatcgc catgaacctc ctggtggact
     1141 tcgcctacgg gctgatcgac ccgcgggtcc gccatacccg acggtgagag aggcagagga
     1201 tgagcgaggc ggaccgcggt ctgttcacgc ccctggaccg ggcggagcgg gccggcctga
     1261 agacccagac caggacgatc gcgttctgga gcgacgtgtg gcgacggttc cggggccacc
     1321 gggccggcat ggccggtctc gtgatcctgg gcctcttcgt ccttctggcg atcatcggtc
     1381 cttccctggt gcgctaccac tacgacatca tcgatctcaa cgagacctac atctatccgg
     1441 gcgcccagca ctggttcggg accgacgtgc tgggccgcga cctgtgggcc cgcgtctgga
     1501 tcggcgcgcg ggtgtccctc ctgatcggcg tggtgtcggc tctgagccag atggtgatcg
     1561 ggacgatcat cggctcgatc tccgggatct acgggggcaa ggtggacatg atcctcatgc
     1621 gcttcgtgga catcctgatc gccgtcccgt tcctgatctg ggtgagcctc ttcatgctcg
     1681 tgctcaaccc ggggatcacc tccatcatcc tcgccttcac ggtcacggag tggaaaggga
     1741 ccgcgaggct ggtgcgcggg gaggtgctgc ggctcaagga gaccgagtac gtcctggcca
     1801 cgcgggggat gggagcgtca acgtggcggc tcatccagag gcacttcttt cccaacgtcc
     1861 ttcccgtgat catcgtctcg gtgaccttcc aggtgacggg agccatcttc ggagaggcgt
     1921 tcctgagcta catcgggttg gggatccagc cccccgtcac gtcgtggggc attctgatct
     1981 cctccgggat cagcgagatg agaagctatc cgtacatgct gttcattccg gccgtgttca
     2041 tcagcctgac gatgctctct cttcagctta tcggggacgc gcttcgggac gccacggacc
     2101 cgaagctgcg gcagtaggcg ggcagccatg ggagacacgg caacccgcag cgccggcgag
     2161 cggctgctcg acgtccgcaa cctcaccatc tccttccaca ccttcgccgg agaggtctcc
     2221 gcggtacggg gggtggactt cgacctcaag cggggagagg tgatcgccat cgtcggggag
     2281 tccggcagcg gcaagagcgt ttccacgcag gccatcctcg gactcacgcc cgtccctccc
     2341 ggagagatca agagcggcga gatcctgttc cagggggaag accttctctc gtacgacgag
     2401 cgccggctca gcgaggtgag cggctcgcgg atctcgatga tcttccagga ccccatgacc
     2461 tccctgaacc cgaccatgaa ggtgggaggc cagatccgcg aggtcctgca ccagcactcc
     2521 cggatgagtc gggaggagag ccgcacgcgc gcgctggagc tcatgaacct ggttggcatt
     2581 ccggagccgt cggtgaggct gggccagtat ccccacgagt attccggcgg catgcgccag
     2641 cgcgccatga tcgccatggc catggccaac cggcccgaga tcctgattgc cgacgagccc
     2701 accacggcgc tggacgtcac catccagtcc cagatcttcc gtctcctccg cggtctgcag
     2761 aaggacttcc acacggcgat catcctcatc acccatgacc ttggcgtggt cgcggggatg
     2821 gcggaccgga tcgtggtcat gtacgcgggc aaggtgatgg aggaggggcg gaccgatcag
     2881 atctactatc gaccctctca tccctacacg gccggcctgc tccaggcggt gccaagcctg
     2941 gccgacgacg cccgtcagaa gcttcggacc atcgagggga ctcctccctt tctcctcaac
     3001 gtcccgcccg ggtgcccctt cgcggagcgg tgtgagtacg ccatgcgggt ctgtcgcgag
     3061 aaagagccgc cctacttccg accggatccg tccaaccgcg tggcctgttg gctgtaccac
     3121 gagggagcgg cggagcagcg gcagaccttc gagaagaggg aacgcgcgcg atgagcggga
     3181 agatcctgga gctgcgcggt ctcacgaagc atttccccat gagccgaggc cggatcctga
     3241 aggcggtaga ggccatcgac ctggatgtgt acgaggggga gaccttcggg ctggtgggag
     3301 agtccggatg cgggaagtcc accctgggcc gcaccatcaa ggggatctac gagcccaccg
     3361 ccggtacgat ccggttccag ggccaggata ccgcgtccat gaccaggccg gagcagaagg
     3421 tctaccggcg caacctccag atcatcttcc aggaccccta ctcctccctc aatcctcgga
     3481 tgacggtccg ggagctgatc ggcgaggggc tggagatcca ccggctggga aaccgcgagc
     3541 ggcagaagcg acgggtgttc gagaccctgg agatggtggg tctcaatccc gagcatgtga
     3601 cgcgatttgc ccacgagttt tccggtggac agcgtcagcg gctcgccatt gcccgcgcgc
     3661 tggcggtgga gccgcggttc atcatctgtg acgagcccat ctccgccctg gacgtttcca
     3721 tccagggaca gatcgtcaac ctcatgaagg acctgcagcg gaagctgggg ctgacgtacc
     3781 tctttatcgc ccacgacctc tcgatggtga agtacatcag cgatcgggtc ggggtgatgt
     3841 acctgggcag gctggtggag gtggccgaga agaacgagct cttccgcaac cctcagcatc
     3901 cttacaccca ggcgctgctc tcctccattc ccatcccgga cccccgcatc gagcggcagc
     3961 gcaaggagat ccccatagaa ggcgagatcc caagcccgat gatgaggatc gatgggtgca
     4021 gtttccgcgc gcgctgccca tttgcggcgc cccgatgcgc ctccatggaa tacacgctga
     4081 aggacatcgg caaccagcac tacgtcatgt gcacgaagtt tccgtaactg agccgcaccc
     4141 tgccggcact ccgccgcgta ccgtggagta gacgcggggg tccgccccca tataatggcg
     4201 atccatgaag gctgctgttc tgcaagagct caacacgatt ggcgtccgcg agacggatcg
     4261 cccgcgctgt ggggacgacg aggccatcct caaggtccac gcgtgcgcgg tctgcgggtc
     4321 cgaccttcgg atcttccact atggcaacga ccgcgtgaag ccgccggccg tgatcgggca
     4381 cgagatcgcg ggccaggtgg tggagacggg aaagaacgtg acccgggtgc acgtgggaga
     4441 tcgcgtggcc gtgggagcgg acgtgccgtg cggcacctgc tactggtgcg ccaacggcat
     4501 ggggaccaac tgccggatca actacgccat cgggtaccag ttccccggcg ggttccagga
     4561 gtacatggtg ctgaaccgga ccatcctggc gtacggaccg gtgactccca tagcagacac
     4621 gctggcatac gccgaggcgg ccatcgcgga gcccctggcc tgcgccatca acgggctgga
     4681 gctcgcgcag ttcggtctgg gcaagagcat ctgtatcatc ggcctggggc cgatcggctg
     4741 catgatgctg gagctgtccc aggtctttgg cgcctccaag gtattcgccg cccagcgctc
     4801 tccgctgcgc atggaaatgg cgcgcccgtt cctgccgcgg gcgcgcttca tcgcgaccga
     4861 gcaggaggac ctggtccaga cggtccttcg cgaaaccgac ggagagggcg ttgaccttgt
     4921 catcaccacg gcgggcaccg tgaaggcgca cgaggacgca gtccagatgg tgcggcaccg
     4981 cggctacgtg aacctcttcg gcgggctgaa gaatcagccc aagctcacta tcgactccaa
     5041 cctcatccac tacaaggaat gtttcgtcat gggaagccac gggtcgttgc cccggcacca
     5101 cgaggccgcc gtcgggctca tctcccgcgg tgccgtgcgc gcgaaggact acctgtccgc
     5161 ggtctacccg ctggagggga tccgtgaagc gctggacttc cacgagtcgc gtaaggggct
     5221 gaaggtgatc gtggcgccgc acggggaaga ggcggcgcgg gcccgcggcg cgggcgcccg
     5281 cggaagggat cccggcgccg gcggggcgct gcggtgagcg gagtcaagat cgcgccgtcc
     5341 ctgatgtgcg cggacttcat ccgccttggc gacgagctgg atgtgatgcg ccaggaggga
     5401 atcgagtacc tgcacctgga catcatggac ggccactacg tccccaacct cgcgctgggc
     5461 cccgacttcg cgaagagggt gcgtgctcac tccccgatcc ccttggacat ccatctcatg
     5521 atcgaggagc cggacaagct gatcccactt tttggcggga ttcccgactg cgtgatctcg
     5581 atccatcccg aagtctccta ccaccccctt cggacgctgc agctcatccg ggacatggga
     5641 gcccggcccg cgatcgcgat cgacccggcg atgcccactt ccgccatcga ggagctcctg
     5701 cctcacgtga cgctggtctg cgtcatgacg gtgaaccccg gctatgcggg gcagaagctg
     5761 atcccggagg ccctgcccaa gatccgccag gtctccgaga tggcggagcg cggcaagctg
     5821 gacatcgagg tggaagtcga cgggaacgtg agctggcaga atgtgccgcg gatggtggag
     5881 gccggtgcgc gggtgctggt ggcgggaacc tcctccttgt acgaccggac cgccggcctt
     5941 cgggagaata tcaaacgcct tcgggccgtg gcgacggctg gggcgccccc ggccgtcgcg
     6001 cctcggcgcg cccaggggtg atcctcgcaa acaccgcgag actggcggcg gtcagcgctg
     6061 cccctgccag gtagaacatc gggtagccca gcgctgcgat cagctggctg cccccggccg
     6121 cggccacgga ccagctcaaa ccgatggcca tgatgtacac accggtcatg ggagcgcgct
     6181 ggcgctccgg gacgacctcc atcaggtaga ccatcagggc cgcgcgtgtg agcgcgatca
     6241 ggcagacgac cacgatgtag ccggcgccgg ccgcggtcca gtgcggcacg agggccagcg
     6301 gaatcagacc cgcgacgatg ccgacggtcc cccactggaa gactcggcgg tggccccaaa
     6361 tcctcacggt ggtcggcatg agaagcgccg ccggcacaga gacgagttgc gcaacggccg
     6421 ccagaagccc gatgcgggcc gtcgaggtat gcagctccgt gtcgaggtac acgttgaaga
     6481 aggtcttggc cgtcccgtct ccgaagccgt gaaggagcag aagcacccca accagcacca
     6541 ggagcatcgc ggagccggag gtttccgctc gggtgggagc cggcgcggcc gtctccgccg
     6601 tcacgggacg ggcgcgggag agcaccacca cggcgacgat gagggtcgcc gctgaggcca
     6661 tcagggagaa ccggtacggt cccggcgccg tcgaggcaga ggttcccgag aggagcccgg
     6721 ggaggaaccc gcccagaaga ctgcccacga acgccgagat gggccacaac gcggcctgca
     6781 gggagaagag gtggctgcgc tcctcccggg tgctggattc caccaggaac ggctgtgagt
     6841 tgacgtcgta gagggagatg gccacgttgg cgaccaggta ggacacgatg agccacaccg
     6901 atcgggcaat ccccgcgcgc atgagctcgc agaagggaac cgccaggtag cccaccatgc
     6961 tgatcaggag gcctgaggtc atcgagcccc gactgcccca tctccgcccc acggaccctg
     7021 ccagcaggca cgccgcggcc cagcccagga gcgccgtcgc gttgatggag ccgatgaagc
     7081 tcaggccgta tcccaggcgt acgagataga ggttgaagag cagggcgtag atgccgccga
     7141 agatcgtaag ccccatcaaa gcagccgtgg cgagatacag ccgcgcgttg cggctgaaca
     7201 tgccgacggc tcgcccccag gaaagcgtcg cggggatctc gacgccgtgc cgcctcaccg
     7261 gtcgaatacc ggcgccagcg cggggtagag catccgatac cgggcgacct gggtctcgta
     7321 cgatcgcgcc agctcgggct ccggttccac cgtctccgcg acccggaccg cctgtgccac
     7381 tccatcctga acgtcccggt agacaccggc cgccgtgccg gcaagaatgg ccgcccccag
     7441 gcagaccgcc tcagggttgc gaagcgtctc cacggtcttg ccggagagcg ccgcgcgaag
     7501 gcgcagccca agacgggagc cggttccacc tcccgtgacc cggacccggg tgaacggcgc
     7561 ggtggcttcc tccagcacgt cggcgttcaa ggcgaactcg caggcaatcc cttcgaggat
     7621 ccccttgtac aggtgaaaga tgcccgcgtc cccggtgagc cccgttacgg ccaccgtggc
     7681 gcgcgggttc cagtgtgggt tgcaggctcc gatgaggtgc ggggtcacgg taagcccgga
     7741 tgggtccgcc ggcgctcggg actcgaacca ttcatgcgga ttcacgcccg aagccacggc
     7801 tcgggcagac tccgcctccg ccagccggtc caggaaccac cgcaccacga tcccggaggg
     7861 gaagaacgcc agggtgacat aggtgtccgg cacgacgtgg cagtaggagt tcaggttggc
     7921 gcgaaaggag gcaggtccca ggcagggggc cgggctcgcc gaatggaagc attcgtaggt
     7981 gccggcggag tccgagacca ccccggggcc gatcgcgccg gcgcccagcg cgcagcacgc
     8041 ctggtcgtga ccgcccacgg caacgatggt acccgccggg acgcccatct cgcgcgccat
     8101 cgccgccgaa aggcggccgg ccgcggtccc cgccgccacg ggccgcggca agcggtcgcg
     8161 ggacacgccg gccgccgcca gaagctcgtc cgaccatcgg tgggtgtgga tgtcgaagac
     8221 cagccaacgg catgccaggg agaagtcggt caggggtggt agacccatgc gccgcaggac
     8281 gtactcgccc ggtccgagga gctgagccgt cgcgctcacc acctcgggac ggtggagctg
     8341 gagccagcgc accttggtca tggaatacat gggatgggcc atggtgccga caatgccgta
     8401 cagacgctct ttgccgatgg tccgttccca ccaggcggcc tcggccacgg cgcgattgtc
     8461 gatgttcatg atggccgggc acaccgggtc cccggtcgcg ttcaccggta tgaaggtctc
     8521 cccgtgggcg ctgatggcga gcgcctgcac cggatcccct cggccttcgg cagcggcggc
     8581 ggcctcccgc accgcctgca ccgttgcgtt ccagagatcc cgtgggtcca tctccgccat
     8641 ggccggtccg gggaacgtca tcgagtagga acggcttgcc gagcagaggg tctctccagc
     8701 cggcgtgaag accgccgcct tgcagctgct ggagcccatg tccacgccga gaaggctcat
     8761 gcatcctcct ggttcaccgc acccggctcc gtcctggcga agaggaccag ctccgagctc
     8821 atgacgttgg gaagccggac cggcagtccc caacgcgcca gctccatacc gctcatcgtg
     8881 accgagcctt gcgtcgcggt ggccgtgtcg gcgctcaggc tgtcgaaggt tacccgatag
     8941 accccgtcca tccgaagtcc ccgcggcttc aggagaaact cctcgggacc ctccccggag
     9001 gtgcggaaca gtcccgccac cgcgtgctcg ccgtcccggg aggcgtactc caggacgcac
     9061 cagggtgagg gtccgtagat cggaagaaac ggagtgtggt gaaagacgcg gctctcgacc
     9121 aggatcggtc gtatgacgcg tcggaacagc tcaagggcgt gatcgatcct ggccttcagc
     9181 gccggggtga gctcgtccag gctgggagcg ataccccgga ggatgggtcg acagagagag
     9241 accaggcgaa gctgcgtctc caggtctccg tccaggtcgt gctcgcccac ctcggtaccg
     9301 aaggtgcgca gcaggatctc cggcgggagg gacacggtaa cgccgttgag gatgcggagg
     9361 cctcgaggca tgcgcatcca gtcgctgagc tcggtgttgt ggaatcgcga cagcgtgccc
     9421 cagtccaggc ggccaccccc tccggcgcag ttctggaaca ctacctcggg aaagcgcgag
     9481 cgaagccggt cgatcatgcc gtagaggttc tcgtagtatc gccaggtgag gtcttccgtg
     9541 tacccatgaa accgccgatt gccggaaggt gtcagcgtgt gattgtgatc gatgcggaag
     9601 aggtccaggc gatactggcg gatgatgcgc tccacctcgg actccgccca gcggacgacc
     9661 tgggggttcg tgaagtccag agcccggccg ttgacgggcg tgccgtcccg acagagaagc
     9721 cattcggcgt gctcccgcct cagcgagctg ttcctgccgg ccgcctcgat ttcgacccac
     9781 agcccgaacc gcatccccag ctcgtgggca tggtccgcta tcggctccag gccgttgggc
     9841 agccaggaac cggctcgcca gtctcccgcg ttgttccacc attcgttcgg gtcgtttccg
     9901 taccaaccgg cgtccaccac gtagagctca gcgccgacgg cctttgccac ctcctcgtcc
     9961 cgcttcaggt ccggctcgtt ctcgcggtcg cacaggtacc cccggtggtt ggcttcgatc
    10021 tccacgtgtc tgccggggat cgggaccggc agcaccgcgg agcgcacgtg gtcatgcgtc
    10081 tcctggacga tgcggtcgac atccgcctga aacagcccca cgtgcacggc cggcgtcctc
    10141 accgtttccc ccggcaggag gacccgcagc gcctcatcct gtccacagag gcccatgcgg
    10201 aagaagagcc tggcccggct cggggagtcg agcaaacgac agtccagagc gaactcgtag
    10261 ttcccacccc aggccagctc gcacaccagc gtctgaccga tggcgatatc cctcgcccag
    10321 aacgcgggtc ttccccagcc gctgcgtccc agcttgccgc cgttcacggt ggtgacgccc
    10381 ggcgcaagcg gctcgaacca gaaatcgccc tcctgacccc actggaagcg gtgggtgtag
    10441 gcgagctgga atggaccaga gacgcccgga ggcagatgct cgtcgatcct ccgggtccag
    10501 atcatccccg cgaacggagc gacagcgctg acgccgatcg gccgggcgga atggttggcg
    10561 accgaaagcc agcgaaccag gaaggggctc ccatccaacc gggtgttgac ggtcacctct
    10621 acgccggacg ccgggtgact caggcggatc gaggccttca ccgggacctg acggtccggg
    10681 ccctgcgccg aggggtccgg ctccgtcgaa gcggaaaccc actggtaccc gccggcaagg
    10741 ctcctgccgt cgattgccag cccgaaggag tctgcgggct cgtcggcttg ccaggcgatc
    10801 gtgccggcgt gcgtctcggg cacgacctgg ccggcagcgg accagtaccg cgtgcacagc
    10861 cggctggccg tgaacgcctc gtccaggacc acggctcccg cgcaataccg catgccgggg
    10921 gagtcaccgg atcgaacaaa cgccctggta ggaacgatcg ccacgtcagg cccttccgct
    10981 ggcatgatgt cggagtgtcg cgggatcggc aaggcccggt tggacgcgac gcggccgccc
    11041 ggcgcgccgc caacgccacc gcgccgcctt gcctcctggg gttggcggtc gctatccttc
    11101 gttattatga cgtgtgcgcc cggagtgcgg gcgcggagga agcggaatgc ggattgcgcg
    11161 agtgcggact gccaacggag cggctcgaca tgcggtgctg gatggagaca ccgcccagct
    11221 catcgaggga gacgtcttca gcacctggaa gcgcgtcggc aagccgttcc cgctgaacga
    11281 ggcgacgctc ctggcccctc tggtgccggt gaacatcctc tgtttcggcc gcaactatcg
    11341 ggcccacgcc gaggagggcg gggcttcgct acccacgcga cccctcctgt tcctgaaggc
    11401 cacctcctgt ttgaacgatc ccgaagctcc catcgtcctt cctcccgccg ctccttccca
    11461 gatcgacttt gaggcggagt tggcggtggt cattggcatg ccggcctacc gggtgtcccg
    11521 ggatcaggcc ctgcggcacg tgttcggcta cacctgcggg aacgatgtga gcgcgcggga
    11581 catccaggag agcgacggcc agtggtcacg ggcgaagtcg ttcgacacct tcggtccctt
    11641 gggtccgtgg atcgagacgc ggctggaccc ggggaacctg cgcatccagg gtcggctcaa
    11701 cggcgaggtg atgcaggatg ccaccaccgc ccagctcgtg ttcgacgttg cctacctgat
    11761 cagcttcctc tcccagggga tgaccctgca gcccggtacc gtcctgatga ccggcactcc
    11821 ggcagggtgc gggtttgctc ggaagccgcc cgtgtggctc gcccccggcg acgtctacga
    11881 ggtggagatc gacggcatcg gcattctccg caaccccgtg aaatcggcct gatcgcgtta
    11941 ccattgaggt agcgcccgcg ggccgcccgc gggggtcaga ggagtcgccg gtgcgtcgga
    12001 tctgtgcctg gtgcaaggcg ccgttgaacc gtgagaaggc gggtcccatc agccatggga
    12061 tctgcgaacc ctgctccgtg tatgtcctct cggagaagtt ccggtttcgg gacttcctgg
    12121 agtccatcga ggcgccgctt cttgccgtcg acggcgaggg gcgcgccatt acggccaata
    12181 ccgtggcgct caagaccctg ggcaagggaa tgccgcaggt cgggggaagg ctccccggcg
    12241 aggtgatgga gtgcgagtac tcccggctgc cgggtggctg cggcaagacc gtccactgca
    12301 acgggtgtca gatccgcatg agcgtcgact acacgcggag cacgggggag gcgcgggtcc
    12361 ggacgcgcgc ctacatccgc atccaggaat cccagggggt gaagcagtac gactactacg
    12421 tcagcacgga gaagctggac ggagtggtcc tgctacggat tgacgagacg cgcccttgcg
    12481 gccagggggg aggcgttccg gccgggaagg ttgatgcgga accgcgagcc ctttgacggt
    12541 tcgctttcgg cctcgatgga cccgccgtgg gcttccacga tggagcgggc gatggaaagc
    12601 ccgatcccgg atcctcccg
//
LOCUS       DASRZS010000445         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2932448, whole genome shotgun sequence.
ACCESSION   DASRZS010000445 DASRZS010000000
VERSION     DASRZS010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2932448"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFH83_10970"
     CDS             complement(<1..294)
                     /locus_tag="VFH83_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486933.1"
                     /translation="MQLFSPFCPSCRESLARNGESFTCSRCGRVYPTKSGVVDFLTSL
                     PLSDVQQVTQKTFDAASPAYDNAIVRLVESLSCPWSTYTARLEAFMSQTGGKVI"
     gene            351..>2152
                     /locus_tag="VFH83_10975"
     CDS             351..>2152
                     /locus_tag="VFH83_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET6486934.1"
                     /translation="MSDTDRDLLNDRQRQAVEATEGPLLIIAGAGSGKTRVITYRVAR
                     LLSKGVPQSQILAVTFTNKAAREMAARVKQLVPRKLARLTVSTFHAFGAQVLRERIEV
                     LGYRPRFSIYDAQDQATLVRETASELGMKGEGFDLQGTVQTLSSIKTGRTPWSDKNRA
                     IRPLYRQYQKNLRLYNAVDFDDLIMLPIELLSKHDAVRSEYQDRYRYLLVDEFQDTST
                     AQYELMRLLVGPQNNVCVVGDDDQSIYSWRGASYENLKRFERDFPGVREIKLEQNYRS
                     TGTILRAANGLITHNSNRKPKELWTGLPEGERIEVTSHESEMEEAEHVVSRIRTLMIR
                     ESLQYQDFGVLIRANHLSRALEEVFRRENLPYVISGGMSFFERQEVRDMLAYLKLLAN
                     PDDETSLLRIVNTPKRGIGRKLIERVAERAEAERCSMFSALSWLAEQPAASAAEEKSH
                     TALKEFLDLVGTYRGRFLEGRQMSQALKELVEEIDYWGHLLVENRDAKDKDVVKWKFG
                     NVESLIGSLADYEEDPDNLDRGLFDYLARVSLASRDDLDSKETGGRINLMTIHSAKGL
                     EFPVVFVAGVEQGILPHARSMEEAEENVEEERRL"
BASE COUNT          434 a          655 c          695 g          368 t
ORIGIN      
        1 gatcaccttg cctcccgtct ggctcatgaa ggcctccagc cgggccgtgt aggtgctcca
       61 gggacaggag aggctctcca cgagccggac aatcgcgttg tcataggccg gggacgctgc
      121 gtcgaaggtc ttctgggtta cctgttgtac atccgagagc ggaagggagg tcaggaaatc
      181 aacgacaccg cttttcgtcg ggtataccct tccgcatcgc gagcaggtga acgattcgcc
      241 gtttcgggcg agactctccc tgcagctcgg gcagaagggc gagaagagtt gcatatgcgc
      301 tattttatgg caccggtacc cgacgtgtca aacagcacga aggatcggcg gtgagcgata
      361 ccgatcggga cctcctgaac gatcgccagc gccaggccgt cgaagccacc gaggggcccc
      421 ttctgatcat cgccggagcc ggatccggga agacgcgcgt gatcacctac cgcgtggcac
      481 ggctgctctc caagggtgtt ccccagtccc agatcctcgc ggtgaccttc acgaacaagg
      541 ctgcccgcga gatggcggcc cgggtcaagc agctggtgcc ccgaaagctc gcgcgcctga
      601 ccgtgagcac ctttcacgcc ttcggggcgc aggtgctgag ggagcggatc gaagtgctcg
      661 gctaccgccc tcggttcagc atctacgatg cgcaggacca ggctaccctg gtccgggaaa
      721 ccgcctccga gcttggcatg aagggtgaag ggttcgacct ccagggtacg gtccagaccc
      781 tatcatcgat caagaccggc cgaaccccct ggtccgataa gaaccgggcc atccgtccgc
      841 tctaccgcca gtaccagaag aacctgaggc tgtacaacgc cgtggacttt gacgacctga
      901 tcatgctgcc gatcgagctc ctgtcgaagc acgatgcggt ccggtccgag taccaggacc
      961 gctaccggta tctcctggtg gacgagttcc aggacacctc tacggcccag tacgagctca
     1021 tgcgcctgct ggtgggcccc cagaacaacg tctgcgtggt cggggacgac gaccagtcga
     1081 tctactcctg gcgcggcgcc agctacgaga acctcaagcg cttcgagagg gactttccgg
     1141 gcgtccggga gatcaagctg gaacagaact accgctctac aggaactatc ctccgggccg
     1201 caaacggcct gatcacgcac aactccaacc gcaagcccaa ggagctctgg acgggtctgc
     1261 cggagggcga acggatcgag gtgacctccc acgagagcga aatggaggag gccgagcacg
     1321 tggtgtctcg gatccggacc ctgatgatcc gcgagtcgct ccagtaccag gacttcggcg
     1381 tgctcattcg cgccaatcac ctgtcgcgag cgctggaaga ggtgtttcgt cgagagaatc
     1441 tcccctacgt catctccggc ggcatgagct tcttcgagcg gcaggaggtc cgagacatgc
     1501 tcgcctacct gaagctgctg gcaaacccgg atgacgagac gagcctcctg cggatcgtca
     1561 atacgccgaa acgggggatt ggtcgcaaac tgatcgagag ggtggcggag cgggcggaag
     1621 cggaacgctg ctcgatgttc tcggcgctga gctggcttgc cgagcagccg gcggcttcgg
     1681 cagcggagga gaaatcccac acggccctga aggagttcct ggatctggta ggcacctacc
     1741 gcggcaggtt cctggagggc agacagatgt ctcaggcgct caaagagctc gtcgaagaga
     1801 tcgactattg gggccacctg ctggtcgaga accgggatgc caaagacaag gacgtcgtga
     1861 agtggaagtt cggcaacgtg gaatccctga tcggctccct ggcggactac gaggaggacc
     1921 ccgacaacct ggaccgcggc cttttcgact acctggcgcg tgtctccctg gccagccggg
     1981 atgacctgga ttccaaggag acgggcggcc ggatcaacct catgaccatc cattcggcca
     2041 agggactgga gtttccggtg gtcttcgtgg cgggagttga gcagggcatc cttccgcacg
     2101 ctcgttccat ggaggaagcg gaggagaacg tggaagagga gcggcgcctg tt
//
LOCUS       DASRZS010000446         3989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1013403, whole genome shotgun sequence.
ACCESSION   DASRZS010000446 DASRZS010000000
VERSION     DASRZS010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013403"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="VFH83_10980"
     CDS             complement(<1..1420)
                     /locus_tag="VFH83_10980"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET6486935.1"
                     /translation="MNDWENPRLLHRERLAPRARFTAYPTEAGAASAVATTGGVSPRS
                     LPLDGLWRFRYGSAPEAVPDGFVERSFDDSAWDRIRVPGNWQMQGYGRPHYTNVIYPF
                     PVDPPHVPSENPTGWYRTSFQLPAAWQGMRILLRFDGVDSAFHVWLNGNPIGFSKGSR
                     IPAEFDLTPAVTQGENTVAVRVCQWSDGSYLEDQDMWWLSGIFRSVCLVAEPMVGIHD
                     IRVATTIGAAGDSADLSVSVELLNSGSARFQGELTFRLIDPDGRPAAEHRAQAVAEAG
                     GATAGGATAGGATAGGATAGGARAGGTARQAVSIPVRAPQLWSAESPSLYGLLVTLRD
                     ARGVEVESVPLRVGLRTVEIRDGHLLVNGRRIIFKGVNRHEHHPDHGRAVPYEAMVRD
                     VQLMKQHNVNAVRTSHYPDDPRFYDLCDRYGIYVIDEADLETHGMEVIGAWSALSDDP
                     EWRDAYVDRGQRMVARDRNHPCV"
     gene            complement(1463..2617)
                     /locus_tag="VFH83_10985"
     CDS             complement(1463..2617)
                     /locus_tag="VFH83_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486936.1"
                     /translation="MQAANPRAQRAANRSLILSELRSHRLASRVDLSRSTGLEKSSIT
                     NIVSDLLEAGLLAEQERGRSATSGGRRAVSLRINERFCSFLGIEIQPRRYRAALLDLT
                     GAQIFRDQGEVHAERDGLGATLQNVYQRLSPGVDRLRVPVAGLGIGVPGYVNPVTGAI
                     SRSIPLGLEHVEFLPPPNPWGVQAWMENDAKCCAWAQLIEPAVGAPRNLLALLMEFQE
                     ANPILGQGAGVSTGFGVVIEDRVYYSNRFASGDFKSLFWRAGHKGQVGILDDRLATIR
                     ADEAVYASYLEEILLHFSPLASVLDPDRIVLCGDAAPSTDRLHAMLQGPLAGTWISQP
                     ENARRIHVSRFGELGVAVGAAGRLLVSLFQPGPTPLVDWETLLAARGAVR"
     gene            complement(2643..3566)
                     /locus_tag="VFH83_10990"
     CDS             complement(2643..3566)
                     /locus_tag="VFH83_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6486937.1"
                     /translation="MRAMLSQNPTSRALGIIGRVVAYLVMGLFALMTITPILWLIMNS
                     FKSNAEYYLSKLSMPQIFQWVNYKGAWDLGDFKELIGNSFIYTIGATLGILFFSILAG
                     FGFAKLPSKATPVLYGSFVMGILLSIQSLMVPLFIEVSQLDKLLGGLFQLLHLVKSAD
                     TFHLFYNSRVGVLIVYIGSGLPIALYLSTEYIKGIPTALVEAARIDGAGFFRIFRTII
                     VPMCVPIATTVAILNITGLWNEFALINILVSKTSLKSIPLGVMRFSGTMMADYGKQFA
                     ALVIGMGPMLVFYIVFRKQITKGVSAGAVKG"
     gene            complement(3570..>3989)
                     /locus_tag="VFH83_10995"
     CDS             complement(3570..>3989)
                     /locus_tag="VFH83_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6486938.1"
                     /translation="AMIPIMFVVLWCWTGMYLILFLANMQKIDPQLLEAARIDGAGEG
                     QVMGKIVLPLLSGVIVNSAILCISGSLNSFALVWAMTGGGPGRVTELMSIYMYRYAFV
                     GVPNFPLANAIAMAIVIFSIVLIVLTKVVERRYGGKE"
BASE COUNT          709 a         1291 c         1361 g          628 t
ORIGIN      
        1 tgacgcaggg atggttgcgg tcccgcgcca ccatccgctg cccgcgatcc acgtaggcgt
       61 cccgccactc ggggtcgtcc gagagagcgc tccaggcgcc gatgacctcc atcccgtgcg
      121 tctccaggtc cgcctcgtcg atcacgtaga tcccataccg gtcgcagagg tcatagaacc
      181 gcgggtcatc ggggtaatgg gaggtccgca ccgcgttgac gttgtgctgc ttcatgagtt
      241 ggacgtcgcg gaccatggcc tcatagggca ccgcgcggcc gtgatccgga tgatgctcgt
      301 gtcggttcac gcccttgaag atgatccgcc ggccgttgac caggaggtgg ccgtcacgga
      361 tctccaccgt gcgcagcccc acgcgcagcg ggaccgactc cacctcgacg ccccgcgcgt
      421 cgcgcagcgt gaccaggagg ccgtagaggc tcggtgactc cgcgctccag agctgaggcg
      481 cgcggacggg gatggagacg gcctggcggg ccgtgccgcc ggctcgggcg cccccggctg
      541 tggcgccccc ggctgtggcg cccccggctg tggcgccccc cgctgtggcg cccccagcct
      601 ccgcgacggc ctgcgcgcga tgctcagcgg cgggacggcc gtcgggatcg atgaggcgga
      661 acgtcagctc cccctggaac cgcgctgagc cggagttcag gagctccacg gaaacgctca
      721 gatccgcgga gtcgccggcc gcgccgatgg tcgtggccac gcggatatca tggatcccga
      781 ccatgggctc ggcaacgagg catacggacc ggaagatgcc gctcagccac cacatgtcct
      841 ggtcttccag gtaactcccg tcggaccact ggcatacgcg gacggcgacc gtgttctccc
      901 cttgggtgac cgcgggggtc aggtcgaact ccgccgggat ccggctcccc ttgctgaacc
      961 cgatgggatt cccgttcagc catacgtgga acgcgctgtc cacgccgtcg aatcgaagga
     1021 ggatccgcat cccctgccac gccgccggaa gctggaagga ggtccggtac cacccggtgg
     1081 ggttctcgga cgggacgtgg ggcgggtcca cggggaacgg gtagatcacg ttcgtgtagt
     1141 ggggcctgcc gtacccctgc atctgccagt tgccggggac ccggatccgg tcccacgcgg
     1201 agtcatcgaa cgaccgttcg acgaatccgt ccgggacggc ctcgggcgcc gacccgtagc
     1261 ggaaccgcca gaggccgtcg agcggaagcg accgggggga tacgccaccc gtcgtggcaa
     1321 ccgcggaggc cgcccccgcc tccgtgggat acgccgtgaa gcgcgcccga ggcgcaagcc
     1381 tctcccgatg cagcagccga ggattctccc aatcgttcat agggacattc tatcggagga
     1441 cgccgagagt gctagccggg ccctatcgaa cggcgccgcg ggcggccagg agcgtctccc
     1501 agtcgaccag gggggtcggg ccgggctgaa agagggatac gagcagccgt ccggcggcgc
     1561 ctacggccac ccccagctct ccgaaccggg agacgtggat gcggcgcgcg ttctccggct
     1621 gggagatcca cgttcccgcc aggggacctt gcagcatggc gtgcagccga tcggtggaag
     1681 gggcggcgtc tccacagagg acgatccggt ccgggtcgag aaccgacgcg agaggagaga
     1741 agtggagcag gatctcttcc aaataggagg cgtagacggc ttcgtccgcg cggatggtgg
     1801 ccagacggtc gtccaggatc cccacctgcc ccttgtgtcc ggctcgccag aacaggctct
     1861 tgaaatcacc gctggcaaac cggttgctgt agtagacacg gtcctcgatg accacgccga
     1921 aaccggtgga tacgccggca ccctggccca ggatggggtt ggcctcctgg aactccatca
     1981 gcagcgccag caggttgcgt ggggcgccca ccgccggctc gatgagctgc gcccaggcgc
     2041 agcacttcgc gtcgttctcc atccatgcct ggactcccca cgggttcggc ggaggcagga
     2101 actccacgtg ctccagcccc aaggggatgg atcgggagat ggcgcccgtg accgggttga
     2161 cgtagcccgg cacgccgatc cccagtcccg ccaccggcac gcgtagccga tccacgccgg
     2221 ggctcagccg ctggtagacg ttctgcaggg tggcgcccag accgtcgcgc tccgcgtgca
     2281 cctctccctg gtcgcgaaag atctgcgcgc cggtcaggtc cagcagggct gcccggtagc
     2341 ggcggggctg gatctcgatg cccaggaagg agcagaaacg ctcgttgatc cgcagggata
     2401 ccgcccggcg cccgccgctg gtcgcggatc tcccgcgctc ctgctcggcg agcaatcccg
     2461 cctccagcag gtcggacacg atgttggtga tggatgactt ctccaacccc gtggatcgag
     2521 acaggtccac gcggctggcc agccggtggc ttcgaagctc cgacaggatc agcgatcggt
     2581 ttgctgcccg ctgggccctc ggattggcgg cctgcatctg gtcctttccg aggggccctc
     2641 gctcacccct tcacggcgcc ggccgagaca cccttggtga tctgcttgcg gaacacgatg
     2701 tagaacacca gcatgggccc catcccgatg accagggccg cgaactgctt gccgtagtcc
     2761 gccatcatcg tgccggagaa ccgcatcact cccagcggga tggacttcaa actcgtcttg
     2821 gagacgagga tgttgatcag cgcgaactcg ttccagagcc ccgtgatgtt gaggatggca
     2881 acggtggtgg cgatggggac gcacatgggg acgatgatgg tgcggaagat ccggaagaat
     2941 ccggcaccgt cgatgcgggc agcctccacc agggccgtcg ggatcccctt gatgtactcc
     3001 gtgctcaggt agagcgctat gggcaggccg gagcctatgt agacaataag gacgcccacc
     3061 cggctgttgt agaagaggtg gaaggtgtcc gcggatttca ccagatggag gagctggaag
     3121 aggcccccca gaagcttgtc gagctgggat acctcgatga agaggggcac catgagggac
     3181 tggatcgaca gaaggatccc catgacgaag ctgccgtaga ggacgggcgt tgccttggac
     3241 ggcagcttgg cgaacccgaa cccggccagg atcgagaaga agaggatgcc aagggtggcg
     3301 ccgatggtgt agatgaagga gttgccgatg agctccttga agtcccccag gtcccacgcc
     3361 cccttgtagt tcacccactg gaagatctga ggcatggaca gcttgctgag gtagtactcc
     3421 gcgttggact tgaaggagtt catgatcagc caaaggatcg gggtgatcgt catgagggcg
     3481 aagaggccca tcaccaggta ggccaccacg cgaccgatga tcccgagggc acgggaggta
     3541 gggttctggc tcaacatcgc gcgcatggtc tactccttcc ccccgtaccg tcgctctacc
     3601 accttggtca gcacgatcag cacgatgctg aagatcacga tggccatggc gatggcgttg
     3661 gccagcggga agttgggcac tccgacgaac gcgtagcggt acatatagat ggacatgagc
     3721 tccgtgacgc ggcctgggcc tccgccggtc atggcccaga ccagggcgaa gctgttgagg
     3781 gaccccgaga tgcagaggat ggcggagttc acgatcacgc cggagagcag agggagcacg
     3841 atcttgccca tgacctggcc ctcccccgcg ccgtcgatgc gggcggcctc caggagctgg
     3901 gggtcgatct tctgcatgtt ggcgagaaag aggatgaggt acatccccgt ccagcaccac
     3961 aggaccacga acatgatggg gatcatggc
//
LOCUS       DASRZS010000447         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2549762, whole genome shotgun sequence.
ACCESSION   DASRZS010000447 DASRZS010000000
VERSION     DASRZS010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2549762"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFH83_11000"
     CDS             complement(<1..304)
                     /locus_tag="VFH83_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486939.1"
                     /translation="MKNRVISRVGIAVVLLLLVGLVGCVMPDQPSSQPPENTYSTGDL
                     LANLDALQGPSARSLSVVGKSVFTLPGGGQLSTQDLASYLSAIKTSFANYASSLTRG"
     gene            complement(534..1073)
                     /locus_tag="VFH83_11005"
     CDS             complement(534..1073)
                     /locus_tag="VFH83_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HET6486940.1"
                     /translation="MDGKAYLASALEEFHKLRELAEKAIAQVSADQLFYAPGPECNSI
                     AIIMKHVGGNLRSRWSDALTTDGEKPYRNRDAEFEKEERDTPQSVRARWDQGWDTVLA
                     TFSGLKPEQVEATMTIRGEPFTVLQATQRSLTHTSQHVGQIVFAAKLVAGSGWKTLSI
                     PRGQSVSFNQAPKPYHRKG"
     gene            1151..2071
                     /locus_tag="VFH83_11010"
     CDS             1151..2071
                     /locus_tag="VFH83_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6486941.1"
                     /translation="MNQTVRLGKTDLMTSEVGIGTNSWGTGRNADPALQATYQAALDS
                     GITFFDTAELYQFGGSERTLGLFLPATSGRWTLASKFFPWPWRLSRGALHAALRRSLD
                     RLAIPRVDLYMTHYPLFPVSIETWMAELARCVQEGLARAVGVSNYSADQLRRAYAALA
                     ARGVTLASNEVEYSLLKRDVERNGTAAACKELGVTLIAYRPLARGVLAGKFSAANPPG
                     GLRGASFTRGFLTRMQPVVELVGRTAAARGKTSSQVALNWLLCKGTLPIPGAHNPDRV
                     RENAGAMGWRLTEAEVRALDDAEERLRRGA"
     gene            complement(2094..>2354)
                     /locus_tag="VFH83_11015"
     CDS             complement(2094..>2354)
                     /locus_tag="VFH83_11015"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET6486942.1"
                     /translation="VENILVIFQSMYEAGRWPVAIYPRWLRFALSFLVPVAFATTVPA
                     EALSGRLTSASLIGAFALAAALFTLARILWRVGIRHYSGASS"
BASE COUNT          404 a          785 c          793 g          372 t
ORIGIN      
        1 ttccccgggt cagcgatgag gcatagttgg cgaagctggt cttgatggcg ctcagatagc
       61 ttgccaggtc ctgcgtgctg agctgcccac cgcccggaag cgtgaatacg gatttgccga
      121 ccaccgaaag acttcgagcg gacggtccct gcagggcatc caggttggcc agcagatcac
      181 cggtcgagta cgtgttttcg ggcggctggc tcgacggctg gtccggcatg acgcagccca
      241 ccaggccgac cagaagcagc agaactacag caatcccgac tcgcgagatc acccggtttt
      301 tcatggttct cctcctcggc gtccagcgtc ccgcggacgc cctccatgag agccgaagag
      361 gtgatcccac tgccgtcagg ggattcccct acggcgctgg gaaagtatcg cacacccgga
      421 ttgcatcgtc aaaccaggtt caccgtcccg aagcaagtcg tccctccggc gaggcgcgtg
      481 gcacgcgggc cagacacgtc aaggggcggg ctgcccgccc atgccggccg gccctacccc
      541 ttgcggtggt agggctttgg cgcctggttg aacgagacgg actgcccgcg ggggatgctc
      601 agcgtcttcc agccggagcc cgcgacgagc ttggccgcga agacgatctg gccgacgtgc
      661 tggctggtgt gggtgagcga ccgctgggtg gcctggagga ccgtaaaggg ctccccgcgg
      721 atggtcatcg tcgcttcgac ctgttccggc ttcaggccgg agaacgtggc caggacggtg
      781 tcccacccct ggtcccaccg cgcgcggacg gactggggcg tgtccctctc ttccttctcg
      841 aactcggcgt cgcggtttcg atacggcttc tcgccgtcgg tagtcaacgc gtcgctccag
      901 cgggagcgca ggttcccacc cacgtgcttc atgatgatcg cgatgctgtt gcactcggga
      961 cccggagcgt agaagagctg gtccgcggac acctgcgcga tggccttctc cgccagctcc
     1021 cgcagcttgt ggaactcctc cagagcgctc gccagatatg ccttgccgtc catgcaatca
     1081 agatacgcac ggcgtggatg gggttccagt ccggctgtac gccgcggggt gcccgcgcta
     1141 taacggggac atgaaccaaa cggtccgact cgggaagacc gatctcatga ccagcgaggt
     1201 ggggatcggc accaactcgt ggggaacggg aaggaacgcc gacccggcac tccaggctac
     1261 ctatcaggcc gcactggatt cggggatcac cttcttcgac acggcggagc tctatcagtt
     1321 cggcggctcg gagcgcaccc tgggcctctt tctaccagcg acctcgggcc ggtggaccct
     1381 ggcctcgaag ttcttcccgt ggccgtggcg cctgtctcgc ggcgccctcc acgccgcgct
     1441 ccgccgcagc ctggatcgcc tggccatccc gagggtggat ctctacatga cccactatcc
     1501 gctcttccca gtctccatcg agacctggat ggccgagctg gcccgctgcg tccaggaagg
     1561 gcttgcccgc gcggtcgggg tgtccaacta ctcggcggac cagctacgcc gcgcctatgc
     1621 cgccctggcc gcgcgaggag tcaccctggc cagcaacgag gtggagtaca gcctgctcaa
     1681 gcgcgacgtg gagcgaaacg gcacagccgc cgcgtgcaag gagctcggcg tcacgctgat
     1741 cgcctatcgc ccactggccc gaggtgtcct ggcggggaag ttctccgcgg ccaatccgcc
     1801 cggcgggctc cggggagcat cgttcacgcg gggcttcctg acgcgaatgc agcccgtggt
     1861 cgagctggtg gggcggaccg cagcggcgcg aggcaagacg tcatcccagg tagcgctcaa
     1921 ctggctcctc tgcaaaggga cgctccccat ccccggagcc cacaaccccg accgtgttcg
     1981 ggagaacgcc ggcgccatgg gatggaggct cacggaagcc gaggtccggg ccctggacga
     2041 cgcggaggaa cgactgcgac gaggcgcctg agcggccgga cgagcgccac gaactacgac
     2101 gaggcgcctg aatagtggcg gatcccaacc cgccagagga tgcgggcaag ggtgaagagc
     2161 gccgcggcca aggcgaaggc tccgatgagg ctggcggagg tcagtcgccc ggacaaggcc
     2221 tccgcgggta ccgtggtggc aaaggcgacc ggcaccagga acgaaagggc gaagcggagc
     2281 caacgagggt agatggcgac gggccatcgg ccggcctcgt acatgctctg gaagatcacc
     2341 aggatgttct ccac
//
LOCUS       DASRZS010000448         3281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2932931, whole genome shotgun sequence.
ACCESSION   DASRZS010000448 DASRZS010000000
VERSION     DASRZS010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2932931"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFH83_11020"
     CDS             <1..480
                     /locus_tag="VFH83_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HET6486943.1"
                     /translation="QQYDGTYGYTRDGSFKIDSNGQLVTSDGYKLLPEVILPEGFVND
                     SLTVSQDGRVTVKVAGSDDPIEVGQLELYRFVNPAGLEAIGENLLKTTNASGDPIAGR
                     PGFDGMGKTLQKFLEMSNVSVVKEMVNMIVAQRAYELNSRAIQTSDSMLATATSLKR"
     gene            493..1317
                     /locus_tag="VFH83_11025"
     CDS             493..1317
                     /locus_tag="VFH83_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HET6486944.1"
                     /translation="MTRRARYGVMALLGFAAALWADAAGGSPDPASPAGGPLTLYVTR
                     VVRLAPGDVSLGRLLVVSGNLSAQASQTLDLVVARLGAQPMVVPARSYQSLVESGMGP
                     DAIVVGTRTVLVPTGSVPQGEDYLLDRLADYLAGQGMLPDTPTELTVEQNQLRGLAPQ
                     SGSPVFQLQTSRGVTEVSFDLQGPGFASVTGRIRLGGSAAGGSPDASYAGASDGVRAG
                     DPVAVLFRKNLITIEMPGRAQGTARIGDSVTVYVPESMRAFSGEVTGGKAVTVDLP"
     gene            1325..2317
                     /locus_tag="VFH83_11030"
     CDS             1325..2317
                     /locus_tag="VFH83_11030"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HET6486945.1"
                     /translation="MAILLLTAAAATWAAPVVRIKDISSLQGVRSNQMLGIGVVTGLD
                     GKGDSSTSALLRSTVSSLLSNFGLSVSPADVRSRNVAVVMVSAELPAFARAGDRVDVT
                     VSSIGDARDLGAGILLQSNLQAANGQVYAVAQGKVLTASDPAAAKTVGSIAQGAIVEK
                     EVLSQFLNNNIVSIVLQNPDFVTAAAVQKAITAAFQGITATSRDPSLIEVTVPDDRRA
                     DVVGFIAQLEALTVTPDAPGKVVIDASNGVIVMGEQVRIGKVAVSYRTARVAVGTPAA
                     SATQNPDLFVINQTTTVDDFVNALKAVGLKADVIIGILQAVDRAGALFGTLVIM"
     gene            2332..2628
                     /locus_tag="VFH83_11035"
     CDS             2332..2628
                     /locus_tag="VFH83_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013243845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HET6486946.1"
                     /translation="MQVGNVGNPFSYPINPSQVKKPAIDKTSDLYKACQDFESLFIKQ
                     MLDAMRQTVNKQDDLLGGGMGQDIFEGMLYDEYAKKMSETAQFGLAEMIYRQIS"
     gene            2791..>3281
                     /locus_tag="VFH83_11040"
     CDS             2791..>3281
                     /locus_tag="VFH83_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6486947.1"
                     /translation="MIRKREIASSFDDNLKAYFNQIKKAKLLTFEEELALSRRIQSGD
                     EEARKQLVESNLRLVVRIAKGFLTPEVSILDLIQEGNLGLMRAAAKYDYRKQVRFSTY
                     ASWWIKQSIVRSLSNKTRVIRLPHRQEEKLRKINKVYNSLSQVLMREPSLQEIAEEIG
                     LGE"
BASE COUNT          624 a         1021 c         1057 g          579 t
ORIGIN      
        1 cagcagtacg acggaaccta tggctacacc cgggacggtt ccttcaagat cgactccaac
       61 ggccagctcg tgacctccga tgggtacaag ctccttccgg aggtcatcct ccccgaaggc
      121 ttcgtcaacg acagtctgac ggtctctcag gacgggaggg tcaccgtcaa ggtggccggt
      181 agtgacgacc ccatcgaggt aggccagctc gagctgtacc ggttcgtgaa tccggccggg
      241 ttggaggcca tcggcgagaa cctgttgaag accacaaacg catccgggga tcccatcgcg
      301 gggagacccg gcttcgacgg aatggggaaa acgctccaga agttcctcga gatgtcgaat
      361 gtctcggtgg tcaaggagat ggtcaacatg atcgtggccc agagggcgta cgagctgaac
      421 tcccgggcca tccagacatc ggactcgatg ttggccaccg cgacctccct gaagcgatag
      481 gcggcgcggc tcatgacgcg cagggcacgg tacggcgtga tggcgctgct cggtttcgcg
      541 gcagcgctct gggctgacgc ggcgggaggc tccccggatc ccgcgtcccc cgcgggtggg
      601 cccctcacgc tgtacgtcac ccgcgtggtg cgcctggcgc ccggcgacgt ctccctgggc
      661 aggcttctgg tcgtgtccgg caacctgtcg gctcaggcca gccagacgct ggatctcgtg
      721 gtagcccggc tcggcgccca gccgatggtg gtgccggctc gctcctacca gtccctcgtc
      781 gagtccggca tgggaccgga cgctatcgtg gtcggaaccc ggaccgtgct ggtgccgacg
      841 ggctcggttc cccagggaga ggattacctc ctggatcgac tggcggatta cctggccggg
      901 caggggatgc ttcccgacac tccgaccgag ctcaccgtgg agcagaacca gctccggggc
      961 ttggcgcctc agagcgggtc tcccgtgttt cagctccaga cctcacgcgg ggtgacggag
     1021 gtcagcttcg acctgcaggg accgggcttt gcatccgtga cgggacggat acggctcggc
     1081 ggctcggcgg cgggcgggag cccggacgcg tcttacgccg gcgcatcgga tggcgtgcgc
     1141 gccggggacc cggtggccgt gctcttccgc aagaacctca tcaccatcga gatgccgggg
     1201 agagcccagg ggaccgcgcg gatcggcgac agcgtcaccg tgtatgtgcc ggagagcatg
     1261 cgggccttct ccggtgaagt cacgggtgga aaggcggtga ccgttgacct cccgtaagcc
     1321 tctgttggcg atcctcctcc tcaccgccgc cgcggcgacc tgggccgccc ccgtagttcg
     1381 gatcaaggac atctcctccc tgcagggcgt gcggagcaat cagatgctgg ggatcggagt
     1441 ggtcaccggg ctcgacggaa agggtgactc ctcgacctcc gcccttctgc gttcgacggt
     1501 gtcctctctt ctctcgaact tcggcctctc ggtatctccc gcggacgtga ggagccgcaa
     1561 cgtggcggtg gtcatggtga gcgcggagct tcccgcgttc gcgcgcgcgg gtgaccgcgt
     1621 ggacgtgacc gtctccagca tcggcgacgc ccgcgacctg ggcgccggca tcctccttca
     1681 gtccaacctc caggcggcca acggacaggt ctatgcggtt gcccagggca aggtgctgac
     1741 cgcatcggac cccgccgcgg cgaagaccgt tggctccatc gcccagggcg ccatcgtgga
     1801 gaaagaggtg ctctcccagt tcctgaacaa caacatcgtc tcgatcgtcc tgcagaatcc
     1861 ggacttcgtc acggcagcgg ccgtgcagaa ggccatcacc gcggcgtttc aggggatcac
     1921 cgcaaccagc cgtgacccgt ccctgatcga ggtcaccgtg ccggacgacc ggcgggcgga
     1981 cgtggtgggg ttcatcgccc agctcgaggc gctgacggtc accccggacg cgccgggcaa
     2041 ggtcgtgatc gacgcgagca acggcgtgat cgtcatgggg gagcaggtcc gtatcgggaa
     2101 agtggccgta tcctaccgga cggcccgggt cgccgtcggc acacccgcgg cgtccgcgac
     2161 ccagaatccc gatctcttcg tgatcaacca gaccaccacc gtggacgatt tcgtcaacgc
     2221 cctcaaggcg gtcggcctca aagccgacgt gatcattggc atccttcagg cggtggacag
     2281 ggcgggagcc ctgttcggca ccctggtgat catgtaagac ccgaggagac catgcaggta
     2341 ggaaatgtgg gaaatccgtt ctcgtatccc atcaacccat cccaggtgaa gaagccagct
     2401 attgacaaga ccagtgactt gtacaaggct tgtcaggact ttgagtcctt gttcatcaag
     2461 cagatgctgg acgcaatgcg ccagacggtg aacaagcagg acgatcttct gggcggtggc
     2521 atgggccagg atatcttcga gggcatgctg tacgatgagt atgccaagaa gatgtcggag
     2581 acggctcagt tcggtctcgc cgagatgatc tatcgccaaa tttcatgaaa agtatagcga
     2641 catctcgtat tcaaaaatgt acagccggac catgctggat acggtaggcg aatgtcgccc
     2701 attaactata tactctacag tgacttgcac tggaatagga acgcccagat cttggcacga
     2761 tagttgcatg tttggggtca ggagcattgg atgatcagga agagggagat tgccagcagt
     2821 ttcgatgaca acctcaaagc ctacttcaac cagatcaaga aggccaagct cctcacgttc
     2881 gaagaggaac tggcactctc tcggcgcatc cagtccggcg acgaagaggc gcgcaaacag
     2941 ctcgtggagt ccaacctccg gctggtggtc cgcatcgcca aaggctttct gacgccggag
     3001 gtctccattc tcgacctgat ccaggaaggg aacctgggcc tcatgcgggc ggccgccaag
     3061 tacgattacc gaaagcaggt ccggttcagc acctacgctt cctggtggat caaacagtcc
     3121 atcgtgagat ccctctccaa caagacgcgc gtgatccgcc tgccgcatcg gcaggaggag
     3181 aagctccgga agatcaacaa ggtctacaac agtctctccc aggtgctcat gcgggaaccc
     3241 tccttgcagg agatcgcgga ggagatcggc ctcggggagg a
//
LOCUS       DASRZS010000449         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_238692, whole genome shotgun sequence.
ACCESSION   DASRZS010000449 DASRZS010000000
VERSION     DASRZS010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238692"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VFH83_11045"
     CDS             complement(<1..699)
                     /locus_tag="VFH83_11045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6486948.1"
                     /translation="MNYRLLGRTGMKVSEVSFGAWAIGASWGPVDDKESMAALNKAVD
                     LGVNFFDTADVYGNGRSERLIAQLLRDRSETIYVATKAGRKLTPHVAEKYTPQAIRKF
                     VEGSLRNLRMDALDLLQLHCPPPSIYYTPEMFEALDQMVKAGKLKNYGVSVEKVEEAL
                     KAIEYPGVKTVQIIFNMFRQRPAELFFREAKSRWVGIIARVPLASGLLTGRMTRTTRF
                     KSDDHRAYNRAGAAF"
     gene            complement(703..1656)
                     /locus_tag="VFH83_11050"
     CDS             complement(703..1656)
                     /locus_tag="VFH83_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486949.1"
                     /translation="MFECKRLPRLVVLCAALAALPGVLFAETAAMPANRGFATVKEIL
                     TRSCSACHDWTGSYDTIVGGGRIIAGSPEQSLLYQKIATDAMPVEGDKLTADEKAFIK
                     GWIAAGASSSELPIAVPAAEGAGASAATAAPAANSSFLGFPNKATFHAVTGFTSSALF
                     LAAGVIGVVHLVDMMNEGHSWRDNPANGWSEDTPGSDATRSREIETVWGNQQNLRWWH
                     VGLLVAGETLYIGDAVTGISMMTSQKPGKLSKHDIHRDAFFVHSALMVAQVGLGFAET
                     YALSTGQHDLMIGLGAAHAAIGLAIPVVMIGAGLENLLLPE"
     gene            complement(1678..>2317)
                     /locus_tag="VFH83_11055"
     CDS             complement(1678..>2317)
                     /locus_tag="VFH83_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486950.1"
                     /translation="DYVSKDVLNTVIDGTLVIPLPKLPGVPATYKFSYSLKQKIPAIK
                     PRVDLLDFSVQPPTREQVRDALVKAGSSVDAGKALGVLRNVLQGRKPAEPVIDPSSID
                     VPLTVSFTIRLSNEARAELLFPKLGYELSVNGERLVTGASTRITREGTTSLITIGNTF
                     SSRSLSKNVRELFSSRSGSFVLHGTASVKLPDEIRKEPLPLDFDEGGSFSMK"
BASE COUNT          390 a          753 c          727 g          447 t
ORIGIN      
        1 gaaggccgcc ccggcccggt tgtacgcgcg gtggtcgtcg gacttgaagc gggtggtccg
       61 ggtcatgcgg ccggtgagca ggccgctggc cagcgggacc cgggcgatga tgcctaccca
      121 gcgggacttc gcctcgcgga agaacagctc cgcggggcgc tggcggaaca tgttgaagat
      181 gatctggacg gtcttcacgc ccgggtactc gatggccttg agggcctctt ccaccttctc
      241 cacgctgacc ccgtagttct tcaacttgcc ggctttcacc atctggtcca gcgcctcgaa
      301 catctccggg gtgtagtaga tggacggggg agggcagtgc agctgcagca ggtcaagcgc
      361 gtccatgcgc aggttgcgca ggctcccttc cacgaacttg cggatggcct gcggtgtgta
      421 tttctccgca acgtgcgggg tgagcttccg tcccgctttg gtggccacgt agatcgtctc
      481 cgagcggtca cgcaggagct gcgcgatcag ccgttcgctg cggccgttgc cgtagacgtc
      541 ggcggtatcg aagaagttga ccccgaggtc caccgccttg ttcagggcgg ccattgactc
      601 cttgtcgtcc accggccccc acgaggcccc tattgcccat gccccgaagc tcacttcgga
      661 gaccttcatg cccgtccgtc cgagaagtcg atagttcatg cgtcactccg gcaagagaag
      721 attttccagt cccgccccga tcatgacgac tggaattgca agcccgatcg ctgcgtgtgc
      781 cgcccccagc ccgatcatca ggtcgtgctg ccccgtggac agcgcgtacg tttccgcgaa
      841 ccccagcccg acctgggcca ccatgagcgc cgagtgtacg aaaaacgcgt cgcggtggat
      901 atcgtgcttc gaaagctttc ccggtttctg gctggtcatc atggagatgc ccgtgacggc
      961 atccccgatg tagagggttt ccccggccac caggaggccc acgtgccacc agcgcaggtt
     1021 ctgctggttt ccccaaaccg tctctatctc ccgcgagcgc gtggcgtcag accccggcgt
     1081 atcctcggac cagccattgg cggggttgtc gcgccagcta tgtccttcat tcatcatgtc
     1141 cacaaggtgc acgacgccga tgacccctgc cgccaggaag agcgcgctgg atgtgaatcc
     1201 cgtcacggcg tggaacgtgg ccttgttcgg aaacccgagg aagctgctgt tcgccgccgg
     1261 tgccgccgtc gcggcggatg cgcctgcacc ttccgcggcg ggtaccgcaa tggggagctc
     1321 ggatgacgat gcacccgcgg cgatccagcc cttgatgaac gccttctcgt ccgcggtcag
     1381 cttgtcgccc tccacgggca tcgcgtcggt tgcgatcttc tggtagagca ggctctgctc
     1441 aggggaaccg gcaatgatcc gcccgccccc gacgatggtg tcgtaggaac cggtccagtc
     1501 gtgacaggcg gagcagctcc tggtcaggat ctccttgacc gtcgcaaaac cgcggttcgc
     1561 cggcatcgcc gcggtctcgg cgaaaaggac cccgggaagc gcggccagcg ccgcacacag
     1621 gacaacgaga cggggtaacc ttttgcattc gaacatggcg gcaagaatac cactctttta
     1681 cttcatcgag aagcttccgc cctcgtcgaa atcgaggggc aggggttcct tgcggatctc
     1741 gtcaggaagc ttgaccgagg cggtgccgtg cagcacgaag ctgccgctcc tgctggagaa
     1801 aagctctcgg acattcttcg aaaggctgcg cgagctgaag gtgtttccaa tggtgatgag
     1861 cgatgtggtc ccctcccgcg tgatcctcgt gcttgccccc gtgaccagcc tctcgccgtt
     1921 caccgacagc tcgtagccaa gcttcggaaa caggagctcg gccctggcct cgttggaaag
     1981 gcgtatggtg aagctcacgg tgagaggcac gtcgatgctc gacgggtcga tcaccggctc
     2041 cgccggcttc cttccctgca gcacgttccg caggacgccc agcgccttgc ccgcatccac
     2101 cgagcttcct gccttgacca gtgcatccct cacctgttcc cgcgtcgggg gctgcaccga
     2161 gaagtccagg agatcgaccc gcggcttgat ggcagggatc ttctgtttca gtgagtacga
     2221 gaacttgtac gtcgcgggaa cgccgggaag cttcgggagc gggatgacaa gggtgccgtc
     2281 gatgacggtg ttcagcacgt ccttcgacac gtagtcc
//
LOCUS       DASRZS010000450         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4474907, whole genome shotgun sequence.
ACCESSION   DASRZS010000450 DASRZS010000000
VERSION     DASRZS010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4474907"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..977)
                     /gene="panB"
                     /locus_tag="VFH83_11060"
     CDS             complement(123..977)
                     /gene="panB"
                     /locus_tag="VFH83_11060"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET6486951.1"
                     /translation="MSELKKVMIPDLMAMKRDGKKISMITAYSFPQGQIVDEAGIDIV
                     LVGDSLGMVELGYRDTVPVTMEEMLSHTRAVTRAVKRAHVVGDLPFMSYNISREQAIT
                     NAGILYKDGGADSVKLEGGQEMAATVQAIVKAGIPVFGHIGLTPQTANVLGGFKVQGK
                     SIDQARKVFDDAVALEEAGAFAIVLEAIPRQLAALITERLKIPTVGIGGGLDCDGQVL
                     VLHDMVGLFRRFTPKFVKVYADVFSVQVDAVKRYVQDVQGRTFPSEEQSFTMKEDSVA
                     ALRSALGL"
     gene            1178..2140
                     /locus_tag="VFH83_11065"
     CDS             1178..2140
                     /locus_tag="VFH83_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET6486952.1"
                     /translation="MSGAITDVPGIEVGHAQNLAGGSGCTVVLCRGGAVPGVAVQGGA
                     PGTRETDSLRPQNVVPVAHAVLLTGGSAFGLSCADGIMRYLEEQGIGLRVGPAIVPIV
                     PGAVLFDLAFADRSARPDAEMGYEACRAASRAERRQGNVGAGTGATIGRLRNNACGMV
                     KGGLGTASLRVGDLVVGVITAVNSNGDVSDPATGRPIAGTLTEDRRAIAGARTILTDP
                     MAPAESDLPTNTTIAVVATNALLSKAAAERVAIMSQDGISRVVDPVHTMGDGDVTFCL
                     GTGTVKADTTRVGAIAAWLLSEAIANAVKAAEPLRGVPCWRDIR"
     gene            complement(2189..>3090)
                     /locus_tag="VFH83_11070"
     CDS             complement(2189..>3090)
                     /locus_tag="VFH83_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6486953.1"
                     /translation="MARLVKDSLVALGPVPEPVILAAMPIAVENGMVSITATTSLEYA
                     QKFFPWTISWFPPTEQRLGMLVAAWGNQFADMKKIVQFVEPYGPWPGMADAHVKGLEG
                     VGKTMLEKVDVPSDVVSVAPLVVKGLQEKPDGIVLVCSGDKAAKIVKELKSRGWKKMD
                     HVLIFSSGDVPELYSTGGKDLNGVEIYNYINPDVSNPRWNAVKAAYAASHNGAEAPSL
                     LINYYDAVYMVRDAIVNQKLTGSPSKLKAERKAISDYISNVKGFQGVMFNWDMKDGVP
                     TNKPTFIFKIEDAKKVLVQEVTQ"
BASE COUNT          519 a         1055 c          989 g          527 t
ORIGIN      
        1 ttgggaccga ggaggcctac gatctcaccc gaaccgaccg tgaaggacac gtcccgaacc
       61 acttccaggg cgccatagct cctggcgacc cggatcgctt cgatcacggg cgcgccctcg
      121 gactacagcc ccaacgcgga gcgaagcgcg gccaccgagt cttccttcat cgtaaaggac
      181 tgctcctccg acgggaacgt cctgccctgc acgtcctgga cgtatcgctt caccgcgtcc
      241 acctgcacgg agaagacgtc cgcgtagact ttcacgaact ttggcgtgaa ccgacggaag
      301 agaccgacca tgtcatggag caccagcacc tgtccgtcgc aatccagacc tcctccaatg
      361 ccgacggtcg ggatcttcaa cctctccgtg atgagagccg caagctgtcg agggatggcc
      421 tcgaggacga tggcgaacgc acccgcttcc tcgagcgcca cggcatcatc gaagaccttc
      481 ctggcctggt cgatggactt gccctggacc ttgaagcccc caagcacgtt ggcggtctgc
      541 ggcgtgagcc cgatgtgccc gaacaccggg atacccgcct tgacgatggc ctggacggtg
      601 gccgccatct cctgcccgcc ttccagcttc acggagtccg cccccccgtc cttgtagagg
      661 atgccggcgt tcgtgatggc ctgctcccgc gagatgttgt acgacatgaa gggaagatcg
      721 cccaccacgt gcgcgcgctt gacggcccgg gtcaccgccc gcgtatggga cagcatctcc
      781 tccatcgtga cggggacggt gtcccgatag ccgagctcca ccatgccgag gctgtccccc
      841 accaggacga tgtcgatccc ggcctcgtcc acgatctgcc cctgcgggaa cgagtaggcg
      901 gtgatcatgc tgatcttctt gccgtcgcgc ttcatggcca tgaggtccgg gatcatgacc
      961 ttcttcagct cgctcattcg gtgcaatcct ccgtgttggt acccagacag tagcgaaaac
     1021 cgccgtggga ctcaatatcg cacgacaccg gacgcgagtc cccgggcgct ccggaggcgc
     1081 cggggccccg agccgagcag cgcgttgaca cgcgccttgc tccccactat ggtctcggcg
     1141 aagccggtct ctcaatccag acaccaaggg gcacagcatg agtggggcta tcaccgacgt
     1201 tcccggcatt gaagtagggc acgcccagaa tctggcaggc ggaagcggct gtaccgtcgt
     1261 cctctgccgt ggcggcgccg ttcccggcgt ggcggtgcaa ggcggagccc ccggcacccg
     1321 ggagaccgac agccttcgac cgcagaacgt ggtcccggtg gctcacgcgg tcttgctgac
     1381 gggaggcagc gcgttcgggc tgagctgcgc cgacggcatc atgcggtatc tcgaggaaca
     1441 ggggatcggc ctgcgggtcg gtcccgccat cgttcccatc gtgcccggag ccgtcctctt
     1501 cgacctggcg ttcgcggacc gctcagcgcg ccccgacgcg gagatgggct acgaggcatg
     1561 ccgagccgcc tcccgcgccg agcggcgcca ggggaacgtg ggagcgggga cgggagccac
     1621 gatcggacgg ctgcggaaca acgcctgcgg gatggtgaag ggcgggctgg gcacggccag
     1681 ccttcgagtc ggcgatctgg tggtcggggt catcacggcg gtgaacagca acggcgatgt
     1741 cagtgaccca gcgaccgggc gccccatcgc cgggaccctg acggaggacc gacgcgccat
     1801 cgcgggcgcc cgtaccatcc tgaccgatcc catggcgccc gccgagagcg acctccccac
     1861 gaacacgacc atcgccgtgg tggccacgaa cgcgcttctc agcaaggccg cggccgaacg
     1921 tgtggccatc atgagtcagg acgggatctc ccgggtggtg gacccggtcc acaccatggg
     1981 ggacggagac gtgaccttct gcctgggcac gggaaccgta aaggccgata cgacccgcgt
     2041 cggcgccatc gcggcctggc tcctgtcaga ggcaatcgcc aacgccgtga aggcggcgga
     2101 gccgcttcgc ggcgtaccgt gctggagaga catccggtag cgcggccgaa tggcgtgggg
     2161 ggcgctgtcg gccccccacc cctgccgact actgcgtgac ttcctggacc aggaccttct
     2221 tggcgtcctc gatcttgaag atgaaggtcg gcttgttcgt cgggacgcca tccttcatgt
     2281 cccagttgaa catgacgccc tggaacccct tcacgttgga gatgtagtcc gagatggcct
     2341 tgcgctccgc cttcagcttg ctggggctgc cggtgagctt ctggttcacg atcgcgtccc
     2401 ggaccatgta caccgcgtcg tagtagttga tcaggaggct cggcgcctcg gctccattgt
     2461 gggaagccgc gtaggcggcc ttgaccgcgt tccagcgcgg gttggagacg tcagggttga
     2521 tgtagttgta gatctcgacc ccgttcaggt ccttgccgcc ggtggagtac agctccggca
     2581 cgtcaccgct ggagaagatc aggacgtgat ccatcttctt ccagccgcgg ctcttgagct
     2641 ccttgacgat ctttgcggcc ttgtccccgc tgcacaccag gacgatcccg tccggcttct
     2701 cctgcaggcc cttcacgacg aggggggcca ccgagaccac gtccgagggc acgtccacct
     2761 tctccagcat cgtcttgccg acgccctcca gacctttcac gtgcgcgtcc gccattccgg
     2821 gccaggggcc gtagggctcg acgaactgca cgatcttctt catgtccgcg aactggttgc
     2881 cccaggcggc caccagcatt cccagcctct gctcggtcgg cgggaaccag gagatggtcc
     2941 acgggaagaa cttctgcgcg tactccagcg aggtggtggc ggtgatcgat accatcccgt
     3001 tctccaccgc gatgggcatg gccgcgagga tgaccggctc gggaaccgga cccagcgcca
     3061 ccagggagtc cttgacgaga cgggccatct
//
LOCUS       DASRZS010000451         4600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4475073, whole genome shotgun sequence.
ACCESSION   DASRZS010000451 DASRZS010000000
VERSION     DASRZS010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4475073"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /gene="nifA"
                     /locus_tag="VFH83_11075"
     CDS             complement(<1..1393)
                     /gene="nifA"
                     /locus_tag="VFH83_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HET6486954.1"
                     /translation="MATERRAAEGRREETSILFEVSQILDSSLDLRTVVEPVLEVITR
                     SLEMKFTTLTLLNRQTGEIFIEAAYGLSPSQKQRGRYKLGEGITGQVIQNGKPAIVPR
                     IDEEPQFLDRTGARRASKGADVSFVCVPIKLGREVVGALSADRPAAPLESLREDARLL
                     SIIASLIAQAVRLRQAAQEERERLLQENLRLRDELKDRFRPSNIIGNSTAMQSVYQLI
                     GQVSTSDTTVLLRGESGTGKELVAHAIHYASRRAAKPFVRVNCAALPEGVIESELFGH
                     ERGAFTGAVAARKGRFELAQNGTLFLDEIGDLSPALQIKLLRVLQEKEFERVGGTSTI
                     KADVRVIAATNRDLEKLIEQERFRQDLYYRLNVFPVHIPPLRERRTDILLLADYFAER
                     YSRQNHKNVRRISTPAIDMLMAYHWPGNVRELENCIERAVLLSDDEVLHGHHLPPTLQ
                     TAEASGTVPRGTLR"
     gene            1580..2836
                     /locus_tag="VFH83_11080"
     CDS             1580..2836
                     /locus_tag="VFH83_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6486955.1"
                     /translation="MNPLAIELNAALEGTTPGALLSGLGKRLYFPKGIVAQSAEAKKQ
                     ATRAGKPVFLPAIRTLVPGLDPDEIFPYAPTQGVEKLRELWKQEIIRKNPDIGSTPFS
                     LPLVVAGLTCGISTLADLFMDRGDVLVLPDLHWDNYPLIFETRRECRLETFPFFTKAG
                     GFNVDGLEDALRRGSASGRVALLLNFPNNPTGYSLLRAEADAVVETIRGVAASGTRCL
                     VITDDAYFGLFYEDGIYGQSLFARLCGLHPNVMAAKVDGSTKEDLTWGFRTGYITLGA
                     KGLTPEQYDAITRKLMGAVRSTVSNSSILAQSLILRAMQSPEREGEKLEAAKLLRQRY
                     ECVKSILSRKRSDAITPLPFNSGYFMSFHLHRGTAEALRKALLATDGIGTIAVEERCL
                     RVAFSSVDTDKLQDLYDRIYAEAEKL"
     gene            complement(2858..3337)
                     /locus_tag="VFH83_11085"
     CDS             complement(2858..3337)
                     /locus_tag="VFH83_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486956.1"
                     /translation="MAIKDPDVFKVVEKMGDHFQTNLSNRFIRKALPMLDLRQSEWER
                     LENLSGEMGTFKMRGFEFDELYETILAAAHFIFQARAKMLPNIRGILAAGSGDSERVL
                     RDMAAQTFPVNLGIFSDLVNELYVKTTNLDRQSHSKTRPVYERIPELKGLGKLLVSG"
     gene            complement(3383..4351)
                     /locus_tag="VFH83_11090"
     CDS             complement(3383..4351)
                     /locus_tag="VFH83_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313015.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HET6486957.1"
                     /translation="MQEPPYRTYSRYLRERHGCAAYRVAVDAGFTCPNRSGGRAGPGC
                     AYCSEDGSRAPHVQDGQSLEAQVRQSIRRLRARRSSAALILYFQAFSNTNAPVERLRA
                     LYERGLEVDEFRGLNVATRPDCIDEAKADLLADFQRRGLDVWVELGLQSGHDETLRRI
                     DRGHTVQQFRDACGVLKGRGLKVAAHLIFGLPGEGRTEILQTVSLVAGLGVDGVKIHN
                     LHVPHGTPMARELLKGELAVPGPWSHLEHVIAALERLPAAVVIMRLVCETPADRLAAP
                     RGFWPKGVFTARLAAEMRRRGAYQGRLVGASAGGSSPSGRLSQPIA"
BASE COUNT          725 a         1565 c         1502 g          808 t
ORIGIN      
        1 cgcgcagcgt cccccggggc acggtgccgc tcgcctccgc ggtctgcagc gtgggcggca
       61 ggtggtggcc gtggaggacc tcgtcgtcgc tgaggaggac ggccctctcg atgcagttct
      121 ccagctcccg cacgttgccc ggccagtggt aggccatgag catgtcgatt gccggtgtcg
      181 agatgcggcg cacgttcttg tggttctgac ggctgtagcg ctccgcaaaa tagtcggcga
      241 ggagcaggat gtcggtgcgc cgctcccgca gcggcgggat gtgcacgggg aatacgttca
      301 gccggtagta gaggtcctgg cggaacctct cctgttcgat cagcttctcg aggtcccgat
      361 tggtggcggc gatcacccgc acatcggcct tgatggtgct ggtgcccccc acgcgctcga
      421 actccttctc ctggaggacc cgaaggagct tgatctggag cgccggggag aggtccccga
      481 tctcgtccag gaagagggtg ccgttctgag cgagctcgaa gcgtcccttg cgcgcggcca
      541 cggcgcccgt gaaggcgccc cgttcatgcc cgaagagctc gctctcgatc acgccctccg
      601 gcagcgccgc gcagttcacg cgcacgaacg gcttggcggc ccgccgactg gcgtagtgga
      661 tggcgtgcgc caccagctcc ttgcccgtgc cgctctctcc ccgaaggagc accgtcgtgt
      721 cgctcgtgga gacctggccg atgagctggt agaccgactg catggccgtg gagttgccga
      781 tgatgttgga cggccgaaac cggtccttga gctcgtcgcg aagccgcagg ttctcctgga
      841 gcagccgctc gcgctcctcc tgggccgcct gccgcaggcg cacggcctgc gcgatcaggg
      901 aggcgatgat ggagagcagc cgcgcgtcct cccgcaggga ctccaggggc gcggcgggcc
      961 ggtccgcgct cagggcaccc accacttccc ggcccagctt gatggggacg cagacgaacg
     1021 agacatcggc gcccttgctc gcgcgccgcg ccccggtccg atccaggaac tggggctcct
     1081 cgtcgatccg gggaacaatg gcgggcttgc cgttctggat gacctggccg gtgatccctt
     1141 cgccgagctt gtagcggccc cgctgcttct ggctcgggga gagaccatag gcggcctcga
     1201 tgaagatctc cccggtctgg cggttgagca gggtcagagt cgtgaacttc atctccaggg
     1261 accgtgtgat cacctccagc accggctcca ccaccgtgcg gaggtccagg ctggagtcga
     1321 ggatctggct cacctcgaag aggatggagg tctcttcccg tcttccttct gcggctcgcc
     1381 gctccgtggc catcgggtct tcccctctct cgacggaact tccgaaatgt aggttccgaa
     1441 aatggaagtt acggaaatgt agcttgccat gcacccgcgc gtcaagggcg ggtccgccgc
     1501 ccaagcggtc cgcggacgcg tgcccgtcgc cgggtgtcgc cgggttgaca ccccgccgcg
     1561 gctcgcgtac aaatcatcca tgaacccact ggccatcgag ttgaacgcgg ccctggaagg
     1621 gacgacgccc ggcgctctgc tctccggtct gggcaagcgg ctgtacttcc cgaaggggat
     1681 cgtcgcccag tccgcggagg ccaagaagca ggccacgcgc gccggcaagc cggtgtttct
     1741 gccggccatc cgcaccctgg taccgggtct ggacccggat gagatcttcc cctatgcccc
     1801 cacccagggt gtggagaagc tgcgggagct gtggaagcag gagatcatcc gcaagaatcc
     1861 cgatatcggc agcaccccgt tctccctgcc cctggtggtc gcgggcctga cctgcggcat
     1921 ctccacgctg gcggatctct tcatggaccg aggagacgtc ctggttctcc cggacctgca
     1981 ctgggacaac tacccgctca tcttcgagac ccgtcgggag tgccgcctgg agaccttccc
     2041 ctttttcacc aaggcaggcg ggttcaacgt ggatgggctt gaggacgcgc tgcgccgcgg
     2101 ctccgcctcg ggacgcgtcg ccctgcttct gaacttcccc aacaacccca ccggctactc
     2161 gctcctgcgc gcggaggcgg acgcggtcgt ggagaccatc cgcggggtgg cggcctccgg
     2221 tacccggtgc ctggtcatca cggatgacgc ctacttcggc ctgttctacg aggacgggat
     2281 ctacggccag tccctctttg cccgtctctg cgggctgcac ccgaacgtga tggccgcgaa
     2341 ggtggatgga tccacgaagg aggatctgac ctgggggttc cgcaccggct atatcaccct
     2401 gggcgccaag ggcctgaccc cggagcagta tgacgccatc acccgtaagc tgatgggcgc
     2461 cgtccgctcc acggtgagca actccagcat cctggcccag agcctgatcc tgcgggccat
     2521 gcagagcccc gagcgggaag gggagaagct ggaggcggcg aagctccttc gccagcgcta
     2581 cgagtgcgtg aagtccatcc tctcgcggaa gcggagcgac gccatcacgc cgctgccgtt
     2641 caactcaggc tacttcatga gctttcatct gcaccggggc acggcagaag ccctgaggaa
     2701 agctctcctg gccacggatg ggatcgggac catcgcggtg gaggagcgct gcctgcgggt
     2761 cgcgttctcc agcgtggata ccgacaagct gcaggatctc tacgatagaa tatacgcgga
     2821 ggcggagaag ctctgagggg gctggctggc cccccgccta gccgcttacc aggagctttc
     2881 caagcccctt cagctcgggg atccgctcgt agaccggccg cgtcttggag tgggattgcc
     2941 ggtccaggtt ggtcgtcttc acgtacagct cgttcacgag gtccgaaaag atgccaaggt
     3001 tgaccgggaa ggtctgcgcg gccatgtccc gaaggacccg ctcggagtca cccgacccgg
     3061 ctgccaggat gccgcggatg ttggggagca tcttggcccg cgcctggaag atgaaatgcg
     3121 ccgccgccag gatggtctcg tagagctcgt cgaactcgaa ccctcgcatc ttgaaggtgc
     3181 ccatctcccc gctgaggttc tccagtctct cccactcgga ctgccgaagg tcgagcatgg
     3241 gcaacgcctt ccgtatgaag cggttggaaa ggttcgtctg gaagtgatcg cccatcttct
     3301 caacgacctt gaatacgtcc gggtccttta ttgccacagc gcgcgcctct tgccccaagg
     3361 gtagaccaac cgccctcgtc tttcaagcga tgggttgcga aagtcggccc gaagggctcg
     3421 atcctcccgc ggatgccccc accaggcggc cctggtaggc gccgcggcgg cgcatctcgg
     3481 ccgccagccg agccgtgaac acgcccttcg gccagaaacc gcgcggcgcc gccaggcggt
     3541 cggccggtgt ctcgcagacc agccgcatga tgaccaccgc tgccggaagc ctctccagcg
     3601 ccgcgatcac gtgctccagg tgactccacg ggccgggcac ggcaagctcc cccttgagga
     3661 gctctcgggc catcggcgtg ccgtggggta cgtggaggtt gtgaatcttc actccgtcca
     3721 cgccaagccc cgccaccagg ctcacggtct ggaggatctc ggtcctccct tcccccggca
     3781 atccaaagat cagatgggcc gccaccttga gcccgcgccc cttcagcacc ccacaggcat
     3841 cccggaactg ctggaccgtg tgtccgcgat cgatgcgccg cagggtttcg tcgtgtccgg
     3901 actgcaggcc aagctctacc cacacgtcca gaccgcgtct ctggaagtcc gccaggaggt
     3961 ccgccttggc ctcatcgatg caatccggcc gggtggccac gttcagccct cggaactcgt
     4021 cgacctccag gccccgctcg tagagagccc gcaggcgctc aacgggcgcg ttcgtgttgg
     4081 agaaggcctg gaagtacagg atcagtgcgg cggagctccg ccgcgctcgc agacgtcgga
     4141 tgctctggcg gacctgagcc tcgaggctct gcccgtcctg aacgtggggt gctcgcgatc
     4201 catcctcgga gcagtacgcg catccgggac cggcgcgccc gccgctgcgg ttgggacagg
     4261 tgaacccggc atccaccgcc acccgatagg cggcgcatcc gtgtcgttcc cgcaggtaac
     4321 gggagtaggt ccggtagggt ggctcctgca ttccttcgcg cgttcctcgc cgcttcactg
     4381 tagcaggatc cggatcccgt tgacaccccg tcaccctcgc ccctacagtg cagcggatga
     4441 agagcctttc gctgcgggtc gcgcttctgg ggatggtggc ctgtgcggcc ctggtggggt
     4501 gcgggctggg cccggtaccc acggtgcagc taatcacaga tcggcccgag atgacggact
     4561 acgtggacca cttcaactct ctgcagtccg acgtccgggt
//
LOCUS       DASRZS010000452         3168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4476349, whole genome shotgun sequence.
ACCESSION   DASRZS010000452 DASRZS010000000
VERSION     DASRZS010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4476349"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VFH83_11095"
     CDS             <1..594
                     /locus_tag="VFH83_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6486958.1"
                     /translation="IVDGAHAFAHLDFKRADLGCDYFCASLHKWLCAPKGTGFLFVRR
                     EKIESVWPLMAAEPDRSADIRKFEETGTRSAATRLAIGEALRFHTCVGGARKEARLRL
                     LRERWTTPLSRMPNVRFPTGLDEDAACGIATVGIDGVSPEALQSHLFANHSILTVAIA
                     HDQVQGIRVTPNVYTTLAELDRFVGVMEQTARHGLPK"
     gene            735..2372
                     /locus_tag="VFH83_11100"
     CDS             735..2372
                     /locus_tag="VFH83_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6486959.1"
                     /translation="MKRLVVLCAAFALLAASLFAGGTQEGKTQAKPGATGPKVLNFPA
                     ASLGGTYYDWMKTVYNRTGQPELVQISLTAYDNNYAVHNIAAESMSVSADGLTWTIKL
                     LRNLQWSDGSPLTADDFVFALQRAALEGYDFGWYWSWAAGIKNWDAVQKKDVPVDQLG
                     VKTIDDYTIQVQTTSPKPYFPGIVSTWYAVPKKQVQKYGDEYATKVETMLCSGPFMLT
                     EWVKDNKMVMVKNPHYHGPWVPIVDQINMYPTLSEAQQGFPAYLAGDIDMTNVDVGQL
                     AVAKDKFPNEIKKNATFQIFYLSYDYSTPPFNDVNVRKAFFYSINRDELTNTVLKDLA
                     IPAHTLVAPGFPGYSEAIAKESVFDPVKAADYLAKAGYPGGKGFPPVTFLWREEGGIT
                     QVTKPMSEYLQAQWKKILGVDITIDSADVKTWMDDLLHLKYKMFLSPYAFDYVDASNF
                     YDLFVTPGSRHNWTNADYNAVVQKADATFKWEDRAPLYQQAEQIMVDQAAIVDLIHPL
                     QYNLVKPYVKGPGVEPNQYGFYPMLSVYTWTHLTVEK"
     gene            2438..>3168
                     /locus_tag="VFH83_11105"
     CDS             2438..>3168
                     /locus_tag="VFH83_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6486960.1"
                     /translation="MELLEVQNLRVSFRQKKQLIQAVRGVSFSIRGERESVGIVGESG
                     SGKSVSGLAIMRLIEEPPGRVSAERLMLSGVDLLAQRDRQMQSLRGREISMVFQEPMS
                     SLDPVFTIGQQLVETIVLHQRATRKQAKEIAAELLRKVEIPEPKHALESYPHELSGGM
                     RQRAMIAIALSCHPRILIADEPTTALDVTVQAQVLQLIKDLQGELGMALILITHDLGV
                     IADTTDRIVVMYGGKILETGSAIDI"
BASE COUNT          634 a          998 c          985 g          551 t
ORIGIN      
        1 atcgtcgacg gcgcccatgc cttcgcccac ctggacttca aacgagcgga cctcggctgc
       61 gactacttct gcgcgtccct gcacaagtgg ctgtgcgcgc ccaagggaac cggcttcctg
      121 ttcgtgcgtc gggagaagat cgagtccgtc tggccgctga tggcagcgga gccggaccgg
      181 tccgcggaca tccggaagtt cgaggagacc ggtacccgct cggcggcgac tcgcctggcg
      241 attggggagg cgctgcgctt ccacacgtgt gtcggtgggg cgcgcaagga ggcgcgcctc
      301 aggctgctgc gcgagcgctg gacaacaccc ctgtcgcgga tgccgaacgt gcgcttcccc
      361 acagggctgg acgaggatgc cgcgtgcggt atcgcgacgg tagggatcga cggagtgtcc
      421 cccgaggcgc tgcaatccca tctgttcgcc aaccacagca ttctgaccgt ggcgatcgcc
      481 catgatcagg tccagggcat ccgggtcacc cccaatgtct atacgacgct cgccgagctc
      541 gatcggttcg tcggcgtgat ggagcagaca gctcggcacg gtctgccgaa gtgatcctca
      601 gggcgctgca acgtcggcgt gccaggcagg cgatgcgggc cgcgcaggcc gccggacaac
      661 ttgacaacgc ttccggcagg ccataggcta gttgcccgat gcatggctcg aatgcgtcct
      721 attgaggagg cggtatgaag agactcgtgg ttctctgcgc ggcgttcgcg cttctggcgg
      781 cctcgctgtt tgcgggcggc acgcaggaag gcaagacgca ggccaagcct ggcgcgacgg
      841 gccccaaggt gctcaacttc ccggctgcca gtctgggagg cacctactac gactggatga
      901 agacggtcta caaccggacc ggtcagccgg agctcgttca gatctccctg accgcgtacg
      961 acaacaacta tgccgtgcac aacatcgccg ccgaaagcat gagcgtctcc gcggacggcc
     1021 tcacgtggac catcaagctc ctgaggaacc tccagtggtc ggacgggagt ccgctcacgg
     1081 cggatgactt cgtgttcgcg cttcagcgcg cggccctgga aggctacgac tttggctggt
     1141 actggagctg ggccgccggg atcaagaact gggacgccgt ccagaagaag gacgtccccg
     1201 tggatcagct cggcgtgaag accatcgacg actacaccat ccaggtgcag accacctccc
     1261 ccaagccgta cttccccggc atcgtctcga cgtggtacgc ggtgcccaag aagcaggtcc
     1321 agaaatacgg ggatgagtac gccaccaagg tcgagaccat gctctgctcg gggccgttca
     1381 tgctcaccga gtgggtcaag gacaacaaaa tggtcatggt gaagaacccg cactaccacg
     1441 gaccgtgggt ccccatagtg gaccagatca acatgtaccc gacgttgagc gaagcccagc
     1501 aagggttccc cgcttacctt gccggcgaca ttgacatgac gaacgtcgac gtggggcagc
     1561 tcgccgtggc gaaggacaag ttcccgaatg agatcaagaa gaacgccacc ttccagatct
     1621 tctatctctc ctacgactat tccaccccgc cgttcaacga cgtgaacgtg cgcaaggcat
     1681 tcttctattc gatcaaccgg gacgagctca ccaacaccgt cctcaaggac ctggccatcc
     1741 ccgctcatac cctggtggcc ccgggcttcc ccgggtacag tgaagccatt gccaaggagt
     1801 ccgtgttcga ccccgtaaag gccgcggact accttgccaa ggccgggtat cccggcggga
     1861 aaggcttccc ccccgtgacc ttcctgtgga gggaagaggg aggcatcacc caggtcacca
     1921 agcccatgtc cgaatacctg caggcccagt ggaagaagat ccttggggtc gacatcacga
     1981 tcgacagcgc ggacgtgaag acctggatgg acgaccttct ccacctcaag tacaagatgt
     2041 tcctttctcc ctacgcgttc gactacgtgg atgcgagcaa cttctacgat ctgttcgtga
     2101 ctcccggcag ccgacacaat tggacgaacg ccgactacaa cgccgtggtc cagaaggcgg
     2161 atgcgacgtt caagtgggag gaccgggcac cgctgtatca gcaggcggag cagatcatgg
     2221 tggaccaggc cgccatcgtt gacctcatcc atccgctcca gtacaacctg gtcaaaccgt
     2281 atgtcaaagg tccgggtgtc gaacccaacc agtacgggtt ctatcccatg ctctccgtgt
     2341 acacctggac ccatctgacg gtcgagaagt aagcaatctc ggcgccgcgc gcgggaggag
     2401 ttcccccctg cgcgcgtgcg cccgtacaac gacaagcatg gaactcctcg aggtccagaa
     2461 ccttcgagtc agctttcgcc agaagaagca actcatccag gccgttcgcg gcgtctcttt
     2521 ctccatccgc ggcgaacggg agagcgtggg aatcgtggga gaatccggct cggggaagag
     2581 cgtgagcggc cttgcgatca tgcgtctcat cgaggagcct cctggccggg tgagcgccga
     2641 aaggctgatg ctgtcgggcg tggacctgtt ggcccagcgg gatcggcaga tgcagagcct
     2701 tcggggccgt gagatcagca tggtgttcca ggagccgatg agctcgctgg atcccgtgtt
     2761 caccattggt cagcagcttg tggagacgat cgtcctccac cagcgcgcca ccaggaagca
     2821 ggccaaggag atcgcggccg agctgctgcg gaaggtcgag atccccgagc ccaagcatgc
     2881 cctggaaagc tatccccacg agctttccgg cgggatgcgc cagagggcga tgatcgccat
     2941 tgccctctcc tgccatccca ggatcctgat cgcggacgaa cccactacgg cgctggacgt
     3001 gacggttcag gcgcaggtgc tccagctcat caaggatctc cagggtgagc tgggcatggc
     3061 tctgatcttg atcacccatg atctgggtgt gatcgccgat acgaccgacc gaatcgtggt
     3121 gatgtacggg ggaaagatcc tggaaacggg gagcgcgatc gatatctt
//
LOCUS       DASRZS010000453         5255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1779541, whole genome shotgun sequence.
ACCESSION   DASRZS010000453 DASRZS010000000
VERSION     DASRZS010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779541"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VFH83_11110"
     CDS             <1..946
                     /locus_tag="VFH83_11110"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6486961.1"
                     /translation="RVKVAGDIQVSFSLKGKSVELKFPKSERYKSLEPPEVSPEFDSD
                     RIQEVVRIYREKMEPRVTESAIAMLRDKVPRAWEEKIIVRLGKSLWIPSTEEDFPSHD
                     PYPDGRIITKADLVRLEQEGGAQPHVITSRLANILYEKTKQQLFSELWTPVLYNEYAV
                     GYLRIWNTLPRRERISRELLDYQQEFAKILCYSLATNGYFRAETSTERRYEAPIIDMS
                     ASGLLFAHTSNDLVRDLMVHNDLPLSFKLEGRTMKIGARIMRKFRDAENVYFGVLFMK
                     IEPEDFAALYQFLYGKPFDPAADGRWEGGAPPPPLVVG"
     gene            956..2068
                     /locus_tag="VFH83_11115"
     CDS             956..2068
                     /locus_tag="VFH83_11115"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6486962.1"
                     /translation="MSAPLSYRERFQRTLLHRAVDRPPMDLGATDMTGIDGGPRRLLP
                     ELGVARPPNATDDEADEAALRALDVDIRDVGGILEPAGTVARQVSPTERVDAWGITSR
                     WNGHHYEAVGRPLAGATVDDLDRYPWPDPERLDPALIRSIADKARRLYEQSPYVVCAR
                     HPYFGVMELGCWMCGFDDFLYRMAAEPDFVHRFFGIVHAYQRRVDEIYYAAVGRYIHF
                     TTSGDDFGAQNAPLVSRTMFVELVCPYLEQRIRHIREFTDASFFHHSCGSIRPLIADL
                     ISAGVQILNPLQPRAASMDPESIKAEFGDSLVFYGAIDTQELLPRASAEEVAEETRRI
                     IRVLGAGGGYVLSAAHTLQGDVPAANIVAMYRAGMP"
     gene            2162..2758
                     /locus_tag="VFH83_11120"
     CDS             2162..2758
                     /locus_tag="VFH83_11120"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6486963.1"
                     /translation="MDEELLRRVAERDPEAVRALYRRYSRIVYGLAARITGSTSDAEE
                     VTQDVFLKVWEKAATYRADRGKVLTWLVQIARNRSVDMLRGQTARGRRDLRLVHQLGT
                     EGATSAPVAPTEEELPVSRERIREALATLSHPQRSALEMAFFQGFTHREISEKLGEPL
                     GTIKTRIRDAMIRLRGLLSEEIRTDNPGGLGVRIQGRG"
     gene            2766..3467
                     /locus_tag="VFH83_11125"
     CDS             2766..3467
                     /locus_tag="VFH83_11125"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET6486964.1"
                     /translation="MQDDHSIRELLPAYALGSLEEREAVRVSHHLQSCEPCRRELSDY
                     QDVSNLLAASPGEVDPPADLEDRVMERILGARTAPKTSALRRIVGFRVSGALAAVIVV
                     ALVAAGTLQFLGLIPSRTQITHAGLSTIVLQGTSAARDAYGTVVLDPEDNHGVLAVRG
                     LPALDSSHQYQLWLVSNGARRSGGVFSVNADGYGSLQITIPADFRDFHTMGITLEPAG
                     GSPGPTTPPVLRGSL"
     gene            3690..>5255
                     /locus_tag="VFH83_11130"
     CDS             3690..>5255
                     /locus_tag="VFH83_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6486965.1"
                     /translation="MKNLGLTVKLLGGFVLVSLVVLVVGYFGMTGAGGLAIGVKDLGG
                     ISVPSVDNLRVVQEDQALIDGAENALMIRARDADAVKDQYGIIDDAKQEQDQAREAYS
                     ALAKSPEAAALWSQATVALDAYWKAHLAFMDLEKQYLQNPTDASYAKLDQHVSDVEDP
                     LSQKVDDLFDQIVTLNHKEVAARVSELTATSARVRLIAVVGLVGGPLIALLLGVTLAL
                     SITRPIARGVGFAQTVAQGDLSQQLGIRQKDEVGRLAEALDGMVGRLKDMIATIMDTA
                     AQVASSSEEISESAQKLSEGAQSQASTLEETSAAVEELAASVDQVAEHAQSQAMAVEQ
                     GTASMSRVEGSIEEVSRNLTAISGLATRSVENSQKGARAVQEVVEGINRIAGSSEKIG
                     GIVSVISDIADQTNLLALNASIEAARAGEYGRGFAVVADEVSKLADRSASSTKEIDRL
                     IKESVGNVAKGVETANGSRAAMEEIREASQQVKDMITGLSRSIAQQVESVKELSAALV
                     KVSEMSQSISAATE"
BASE COUNT          945 a         1690 c         1756 g          864 t
ORIGIN      
        1 gcgcgtgaag gtggcgggag acatccaggt ctccttctct ctgaagggaa agagcgttga
       61 gctgaaattc cccaagagcg agcgctacaa gtccctggag ccgccggagg tctccccgga
      121 gttcgattcc gatcgcatcc aggaggtggt ccggatctat cgggagaaga tggagccccg
      181 ggtcacggag agcgccatcg cgatgctccg ggacaaggtc cctcgcgcct gggaggagaa
      241 gatcatcgtc cgcctcggca agagcctctg gatcccctcc acggaggagg acttcccctc
      301 ccacgatcca tacccggacg gccggatcat caccaaggca gacctggtcc gtctggaaca
      361 ggagggcggc gcacagcctc acgtcatcac gagccggctg gccaacatcc tctacgagaa
      421 gacaaaacag cagctcttct ccgagctgtg gacgccggtg ctgtacaacg agtacgccgt
      481 gggttatctg aggatctgga acaccctgcc gcggcgggag cggatctccc gtgagctcct
      541 ggactaccag caggagttcg ccaagatcct ctgctactcc ctcgccacca acggctactt
      601 ccgcgcggag acctcgaccg agcggcgtta cgaggcgccc atcatcgaca tgagcgcatc
      661 cggcctgctc ttcgcgcaca cgtccaacga cctggtgcgc gacctgatgg tccacaacga
      721 cctccccctc tccttcaagc tggaggggcg cacgatgaag atcggcgcgc ggatcatgcg
      781 caagttccgc gacgcggaga acgtctactt cggggtgctg ttcatgaaga tcgagcccga
      841 ggacttcgcc gcgctctacc agttcctcta tggcaagccg ttcgatccgg cggcggacgg
      901 ccgatgggaa gggggcgccc cgcctccgcc cctggtggtc ggataggggc ccaccgtgag
      961 cgcacccctc agctaccgag agcggttcca gcggactctc ctgcaccgcg ccgtagaccg
     1021 gccacccatg gacctgggcg ctaccgacat gaccggaatc gatggcggcc ccagaagact
     1081 cctccccgag ctgggagtcg cccgtccgcc gaacgccacg gacgatgagg cagacgaggc
     1141 cgcccttcgg gcgctggacg tggacatccg ggacgtcggc gggatcctcg agcctgcggg
     1201 gaccgtggcc cgtcaggtgt cgccaaccga gcgcgtggat gcgtggggca tcacctcccg
     1261 atggaacggg caccactacg aggcggtggg gcggcccctg gccggagcca ccgtggacga
     1321 cctggaccgc tacccgtggc cggatcccga gcggctggat cccgcgctca tccgctctat
     1381 cgccgacaag gctcggcggc tttacgagca gtctccgtac gtcgtctgcg cgcgccatcc
     1441 gtatttcggc gtgatggagc tgggatgctg gatgtgcggg ttcgacgact tcctctaccg
     1501 catggccgcg gagcccgatt tcgtccatcg attcttcggc atcgtgcacg cgtatcagag
     1561 gcgcgtagac gagatctatt acgccgcggt tggacgctac atccacttca ccacgagcgg
     1621 cgatgacttc ggcgcccaga acgcgccgct ggtctcccgg accatgttcg tggagctcgt
     1681 gtgcccgtac ctggagcaac ggatccgtca catccgggag ttcacggatg cctccttctt
     1741 ccaccactcc tgcgggtcca tccgcccgct catagcggac ctcatctccg cgggcgtgca
     1801 gatcctcaac cctctgcagc cgcgcgccgc ctccatggac ccggagtcca tcaaggccga
     1861 gttcggcgac agcctggtgt tctacggagc catcgacacc caggagctcc tgccccgcgc
     1921 cagcgctgag gaggttgccg aggaaacgag gcgcatcatc cgggtgctgg gggccggggg
     1981 cggatacgtc ctgagcgccg ctcacaccct ccagggtgac gtaccggcgg cgaacatcgt
     2041 cgcgatgtat cgagccggga tgccctgagg cgcgctccgc ccgcttgctt tggcgccggg
     2101 cgttcggtac tttcgcctca tggtgcaagg ggcggcaccg tcggacgata cacctcaacg
     2161 catggacgag gagcttctgc gccgcgtggc ggagcgggac ccggaggcgg tgcgcgcgct
     2221 ctaccggcgg tattcgcgga tcgtctatgg ccttgcggct cggatcaccg gcagcacctc
     2281 ggacgcggaa gaggtgaccc aggacgtctt tctgaaggtg tgggagaagg cagcgacgta
     2341 ccgagcggat cgcgggaagg tgctcacgtg gctggtgcag atcgcccgca accggtcggt
     2401 ggacatgctg aggggacaga cggctcgagg caggcgggac cttcggctgg tgcatcaatt
     2461 ggggacggaa ggggcgacct cggcgccggt cgcgcccacg gaagaggagc taccggtttc
     2521 tcgggagcgg atccgggaag cgctggccac gttgagccac cctcagcgct cggcgttgga
     2581 gatggcgttc ttccagggat tcacgcaccg cgagatctcc gagaagctgg gggaaccgct
     2641 gggcacgatc aagacccgca ttcgggatgc catgatccgt ctccgagggc tgctctcgga
     2701 ggagatacgg acggacaatc ccggagggct gggcgtccgt atacagggta gaggctagac
     2761 ggtccatgca ggacgatcac tccatccgcg agctcctccc cgcctacgcc ctcggctccc
     2821 tggaggagcg agaggcggtc cgggtaagcc atcatctcca gagctgtgag ccctgtcgcc
     2881 gcgagttgtc cgactaccag gacgtctcga acctcctggc ggccagcccc ggtgaggtgg
     2941 atccgcccgc ggacctggag gaccgggtca tggagcggat cctcggcgct cgaacggctc
     3001 cgaagacgag cgcgctgcgg cggatcgtcg ggttccgcgt ttcgggggcc ctggccgccg
     3061 tcatcgtcgt ggcgctcgtt gcagccggca cgctccagtt ccttgggctc atcccgtcca
     3121 ggacgcagat cactcacgct ggtttgtcta ccatcgtact gcaaggtacc tccgcggctc
     3181 gggatgccta cgggaccgtg gtgctggatc ctgaggacaa ccatggggtc ctggcggtgc
     3241 gtggcctccc ggctctggac tcgtcccacc agtaccagct ttggctggtg tccaacgggg
     3301 caagacgcag cggtggggtc ttcagcgtca acgcggatgg gtacggatcg ctgcagatca
     3361 ctattcccgc ggacttccgt gatttccaca ccatgggaat caccctcgag ccagccggag
     3421 ggagccccgg gcctaccacg cctcccgtgc tgcggggctc gctgtaatca aggcgacaaa
     3481 cactcgttcg tttaccagac ggtaatcggc cagccaaggg ctatactata gacagatcga
     3541 cgaggaggtt gccgactccg gaagtcctcc tccgtcgcta tttcgttccc tgacaggcag
     3601 ttctgacgca agctgagcac ctggcgtttc cccgtgatgg attgccgtgc ctgcgcccgt
     3661 ggcgcagaag acgtaaggag gctggtgtga tgaagaacct cgggcttacc gtgaagctcc
     3721 tcggaggctt cgttctcgtg tccctggtgg tgctcgttgt cggctacttc ggcatgaccg
     3781 gggcgggcgg gctcgccatc ggcgtcaagg acctcggcgg aatcagcgtt cccagcgtcg
     3841 acaaccttag ggtggtccag gaagaccagg cgctcatcga tggcgcggag aacgccctga
     3901 tgatccgggc cagggacgcg gacgcggtca aggaccagta cgggatcatc gacgatgcca
     3961 agcaggagca ggatcaagcc cgtgaggcat actccgctct ggccaaaagc ccggaggccg
     4021 cagccctctg gagccaggcc accgtggcgc tcgatgcgta ctggaaagct catctggcct
     4081 tcatggacct cgagaagcag taccttcaga acccgacgga cgcttcctac gccaagcttg
     4141 accagcacgt ttcagacgtc gaggaccctc tgagccagaa ggtagacgat ctgttcgatc
     4201 agatcgtcac cctgaaccac aaagaggttg cggcccgcgt gtccgagctg accgccacat
     4261 cggctcgcgt gcgcctcatc gccgtcgtcg ggctggtcgg cgggcctctc atcgcgctgc
     4321 tcctgggagt gaccctggcc ctctcgatca cgcgacccat cgcccgaggc gtggggttcg
     4381 ctcagacggt cgcccaggga gacctctcgc agcagcttgg gatccggcag aaggacgagg
     4441 tcggcaggct ggccgaggca ctggacggaa tggtgggcag gctgaaggac atgatcgcca
     4501 cgatcatgga caccgccgcc caggtcgcct cctccagcga agagatcagc gagagcgcgc
     4561 agaagctgtc cgaaggtgcg cagagtcagg catccacgct ggaggagacc agcgcggcgg
     4621 tggaggagct tgcggcgtcc gtcgaccagg tggcggagca tgcccagtcc caggccatgg
     4681 ccgtagagca aggcacggcc tccatgtccc gggtggaagg ctccatcgag gaggtctcca
     4741 ggaacctcac ggccatctcg ggattggcca ctcggagcgt cgagaactcc cagaagggcg
     4801 ccagggcggt ccaggaggtg gtcgagggca tcaaccggat cgcgggaagc tcggagaaga
     4861 tcggcgggat cgtcagcgtc atttcggaca tagccgacca gaccaacctc ctcgcgctga
     4921 atgcctcgat cgaggcggcg agagccggcg aatacggccg cgggttcgcc gtggtcgccg
     4981 atgaggtgag caagctggcg gaccgaagcg cctcttctac aaaggagatc gaccggctga
     5041 tcaaggagag cgtagggaac gtcgccaagg gcgtcgagac ggccaacggg tctcgcgccg
     5101 cgatggagga gatccgggaa gcgtcgcagc aagtgaagga catgatcacc gggctttccc
     5161 gttccatcgc gcagcaggtg gagtccgtga aggagctttc ggcggccctg gtgaaggtga
     5221 gcgagatgag ccagagcatc tccgcggcca ccgaa
//
LOCUS       DASRZS010000454         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3319701, whole genome shotgun sequence.
ACCESSION   DASRZS010000454 DASRZS010000000
VERSION     DASRZS010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3319701"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="VFH83_11135"
     CDS             complement(<1..1358)
                     /locus_tag="VFH83_11135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HET6486966.1"
                     /translation="MIRKQKALLVESLGSILLEYAFENRYVLHPARLGEIAAALAEDA
                     LAFLDAPDPSGAAAMGTQLATQGVGEKSILNLASGLRGFCRRVQLDNPEGGERGRDAL
                     AASVEAYTTALLQGYMVADKARVLSDQEQLRRALSAALVSQTEELLVKNHAINTSINA
                     IILSDLHGTVTWVNSSFLQMWGFGGQEIPGLSIGDFWNGEEGKGIVEGVSRSGGWRGQ
                     LTARRKDAGTFSVDVTASLIRNEQGDAIGMMTSVVDITEHLKAEEMAKNLLLVKEIHH
                     RIKNNLQVISSLLYLQSGYVQDKRTREMFKESQDRVRSMALLHEKLYQSRSLAGVAFS
                     AYITDLTRNLFQSYGVDSGGTTLELEAADVTLGMDTAVPCGLIINELVTNSLKHAFRS
                     REGGKVVISMKPADPPADPPAGAGSSTDGKWYVLRVSDNGSGFPKDLDFRNTDSLGLK
                     LV"
     gene            complement(1422..>2249)
                     /locus_tag="VFH83_11140"
     CDS             complement(1422..>2249)
                     /locus_tag="VFH83_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017296771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6486967.1"
                     /translation="TEDGILEFYWGEHIHLGHYGTPARPKDFRGAKEDFVREMVRWGG
                     LDRLPPGTTVLDVGCGIGGSSRMLAREYRFDVTGVTISPHQVRRARELTPPDLSVRFQ
                     VDDAMALSFADASFDVVWSVEAGPHMPDKAIFARELLRVLRPGGLLVVADWNQRDDRR
                     APLRLWERAVMRQLLDQWSHPAFSSIEGFSELLAATGLVAGAVVTADWTQATLPSWVD
                     SVRQGVLRPQGLVKFGLTGFIKSMREVPTFLLMRLAFGAGLCRFGMFRAVRAERAGR"
BASE COUNT          386 a          774 c          720 g          369 t
ORIGIN      
        1 cacaccagct tgagtcccag ggaatccgtg ttgcggaagt ccaggtcctt gggaaacccg
       61 ctgccgttgt ccgacacccg aagaacatac cacttcccgt cggtcgagga accggcgccc
      121 gccggcgggt ccgccggcgg atccgccggc ttcatggata tgacgacctt gccgccctct
      181 cgggagcgga acgcgtgctt cagggagttg gtcaccagct cgttgatgat gagcccgcag
      241 ggcaccgcgg tgtccatgcc cagggtcacg tccgcggcct ccagctccag cgtggtcccg
      301 ccgctgtcca cgccgtagga ctggaacagg ttgcgggtca ggtccgtgat gtaggcggag
      361 aacgcgaccc cggccaggct tctggactgg taaagcttct cgtgcaggag ggccatggac
      421 cggacccggt cctggctctc cttgaacatc tcccgcgtcc tcttgtcttg cacgtagccc
      481 gactggaggt acaggaggct cgagatgacc tggaggttgt tcttgatccg gtgatggatc
      541 tccttgacca gcaggagatt ctttgccatc tcctccgcct tcaggtgctc ggtgatgtcc
      601 acgacggagg tcatcatccc gatggcgtcg ccctgctcgt tgcggatgag ggaggccgtg
      661 acgtccacgc tgaacgtgcc ggcatccttg cgtcgggccg tgagctgacc gcgccacccg
      721 ccggagcgcg agacgccctc cacgatgcct ttcccctcct ccccgttcca gaagtctccg
      781 atggagagcc ccgggatctc ctggccgccg aacccccaca tctggaggaa ggacgaattg
      841 acccaggtca ccgtgccgtg cagatcggag aggatgatgg cgttgatcga ggtgttgatg
      901 gcgtgattct tgaccagcag ctcctcggtc tggctgacca gcgcggccga cagagcgcgt
      961 cggagctgct cctggtcgct caggactcgg gccttgtccg ccaccatgta cccctgcagg
     1021 agcgcggtgg tgtatgcctc cacggaggct gccagcgcgt cccgtccccg ttcaccccct
     1081 tcggggttgt cgagttggac gcgccggcag aagccccgga ggccggacgc gaggttcagg
     1141 atgctcttct cgccgacgcc ttgcgtggcg agctgggtcc ccatggccgc ggccccgctg
     1201 ggatccgggg cgtccagaaa ggccagggca tcctcggcca gggcggcagc gatctcaccg
     1261 agcctggccg ggtggaggac ataccgattc tcgaaagcgt attccaggag gatggagccc
     1321 agggactcaa cgaggagagc cttctgcttt cggatcactt gggaaaagac cccatatcac
     1381 gatagcggaa accggagtca tccgcaagct gtcgggcagg gttaccggcc cgcgcgctcg
     1441 gcgcgaaccg ctcggaacat cccgaaccga cacagcccgg cgccgaacgc caaacgcatc
     1501 aacagaaacg tcgggacttc ccgcatggac ttgatgaagc ccgtcaaccc gaacttcacg
     1561 agcccttgcg gccgcaggac accctggcga acggaatcca cccaggacgg cagcgtagcc
     1621 tgcgtccagt ccgcggtgac gaccgctccc gcgaccagcc ccgtggcggc caggagctcc
     1681 gagaatccct cgatgctgga gaaggccggg tgagaccact ggtccaggag ctggcgcatg
     1741 acggcgcgtt cccagaggcg caggggcgcc cgccgatcgt ccctctggtt ccagtccgcc
     1801 accaccagca acccgcccgg tcggagcacg cgaagcagct cccgagcgaa gatcgccttg
     1861 tccggcatat ggggccccgc ctcgacggac cacaccacgt cgaagctggc gtccgcaaag
     1921 gagagcgcca tggcatcgtc cacctggaag cgcaccgaaa gatcgggtgg cgtcagctcg
     1981 cgagcccgcc ggacctggtg cgggctgatc gtgacccccg taacgtcgaa ccggtattcc
     2041 cgcgccagca tccggctgct gcccccgatg ccacagccca cgtccaggac cgtggtgccc
     2101 gggggcagcc ggtccagccc gccccaccgc accatctcgc gcacgaagtc ctccttcgcc
     2161 ccgcggaagt ccttgggtcg agccggagtg ccgtagtggc cgagatggat atgctctccc
     2221 cagtagaact cgaggattcc gtcctcggt
//
LOCUS       DASRZS010000455         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5632846, whole genome shotgun sequence.
ACCESSION   DASRZS010000455 DASRZS010000000
VERSION     DASRZS010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5632846"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VFH83_11145"
     CDS             complement(<1..756)
                     /locus_tag="VFH83_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486968.1"
                     /translation="MIDTSRLLAAFGEADITPSLGTNLAGWIVPLRPAQVVRDPIQAH
                     IGVLQRGADSVAVIGLDILSISIADARDLRERVSRVSGVRAANVLVAASHSHTGPAVA
                     DLPITPSDRGYVEQMKAKIAGKAAELAGHLVPASIGHACGFEGKLSFNRRYIRQDGIV
                     RTHPTPADPQMVCAEGPIDPQLGVLCVRNEHGAALGYLVNFACHPCFLGGLTIVSANY
                     PGSMSRALKALEGPDCVTVFLNGACGDIHHANPV"
     gene            complement(761..2041)
                     /locus_tag="VFH83_11150"
     CDS             complement(761..2041)
                     /locus_tag="VFH83_11150"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6486969.1"
                     /translation="MATKQKSALAIRGGRKAVPDGFIKPWPEIRQEDKDAVMRVLDRG
                     ILWGGTAPEVTKLQEEWARYIGTKYCLATNSGTAALHMAVAGVGIQPGDEVITTAFSW
                     TSTATCILHHNGIPVFVDIKPDTMIIDEDKIEEKITDKTRAIIPVHLYGLSCDLDKVM
                     AIARRHNLKVIEDCCQAHGAEYRGKRVGRIGNVGAFSLNGNKNLAAGDGGLLVTDDEE
                     VYQAAARVQQFGENRHEDGSRDYNAYGMGWMYRTTEMTAAFARSQLARLDWSNGVIWQ
                     NVHHFGDAVRDIKGMRLPVELPERKHCFYEYKIRFYPEDLGLSMPPDQFKQKVEKALS
                     AEGVQVGRWEFLIPDMTLFYQKEGYGKGCPWTCQHYGRNIVYRREDYPVAADIINRCT
                     GIFGLRSPNGFPLMDCYAEAFHKVFGHLEEVLEV"
     gene            complement(2095..3156)
                     /locus_tag="VFH83_11155"
     CDS             complement(2095..3156)
                     /locus_tag="VFH83_11155"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET6486970.1"
                     /translation="MTVIDSHLDLAWCALAWNRDLTATVEEIRRSEVGLSDLRRGHNT
                     VSFPELHRAEVAVCLATVLSRCSTLDDPFLDRRTREIAAAEGAAHVEYYRIMEAAGHL
                     RMVRDAASLDAHWSEWQRTGGAGTPLGFILAMEGADPILRPNDVARWWERGLRVVEPV
                     HYGISFYAHGTGAPGGLTPPGRDLLAAMESAGMILDITHMADGSFWESLDLFHGPVMA
                     SHHNCRALVPGDRQLSDDQIRALVARGGVIGAAFDSWMLYPGYARGETDNSLVSLERV
                     IDHIDHVCQIAGNARHAAIGSDLDGGFGTEQSPRELDTIADLQQVPDRLRRRGYGDAD
                     VEAVMHGNWLRFFREALPR"
BASE COUNT          510 a         1132 c         1063 g          577 t
ORIGIN      
        1 cacggggttg gcgtggtgaa tgtccccgca ggccccgttg aggaagaccg tgacacagtc
       61 cgggccctcc agcgccttga gggcccggct catgctgccc gggtagttgg cactgacgat
      121 ggtgagccct cccaggaaac agggatggca ggcgaagttg acgagatacc ccagagcggc
      181 accatgctca ttccggacgc agaggacgcc cagttggggg tcgatcggcc cctcggcgca
      241 gaccatctgg ggatccgcgg gggtcgggtg ggtccggacg atcccgtcct gtcggatgta
      301 gcgccggttg aagctcagct tcccttcgaa cccgcaagca tggccgatgg acgcggggac
      361 gagatgcccc gccagctccg ccgccttgcc ggcgatcttc gccttcatct gctccacgta
      421 tccacggtca gacggcgtta tggggagatc cgccaccgcc gggccggtgt ggctgtggga
      481 cgcggccacc aggacgtttg ccgcgcgcac gccgctgacc cgggagactc gctccctcag
      541 gtctcgcgcg tccgcgatgg agatgctgag gatgtccagt ccgatgaccg ccacggaatc
      601 cgcaccccgc tgcagcacac cgatgtgcgc ctggatgggg tcccgaacga cctgggccgg
      661 ccgaagcggc acgatccagc ccgccaggtt ggtcccgagg ctcggagtga tgtccgcttc
      721 tccgaaggca gccagaagcc ggctcgtatc gatcacccgc tcacacctcc aggacttcct
      781 ccaggtgacc gaacaccttg tgaaacgcct ccgcgtagca gtccatgagc ggaaacccgt
      841 tggggctgcg aagaccgaag atgccggtgc accggttgat gatatcggcg gccacggggt
      901 aatcctcccg ccgatagacg atgttccttc catagtgctg gcaggtccac gggcaaccct
      961 tcccgtatcc ctccttctga tagaagagag tcatgtcggg aatcaggaac tcccagcggc
     1021 ccacctgcac tccctccgcg gacagggctt tctccacctt ctgcttgaac tggtccggcg
     1081 gcatcgagag cccgaggtcc tcggggtaga accggatctt gtactcgtag aagcagtgct
     1141 tgcgctcggg gagctccacc ggcaggcgca tgcccttgat gtcacggacc gcgtccccga
     1201 agtggtggac gttctgccag atgacgccgt tggaccagtc cagccgcgcg agctgcgatc
     1261 gggcgaatgc cgcggtcatc tccgtggtcc ggtacatcca gcccatgccg taggcgttgt
     1321 agtcccggct gccgtcctcg tggcggttct cgccgaactg ctgcacgcga gccgccgcct
     1381 ggtagacctc ctcgtcatcc gtcaccagga gcccgccgtc gccggctgcc aggttcttgt
     1441 ttccgttgag actgaacgcc ccgacgttgc cgatccgccc cacgcgtttg ccccggtact
     1501 cggcgccgtg cgcctggcag cagtcctcga tcaccttgag gttgtgcctg cgcgcgatgg
     1561 ccatcacctt gtccagatcg caggacagcc cgtagagatg gaccgggatg atcgccctgg
     1621 tcttgtcggt gatcttctcc tcgatcttgt cctcgtcgat gatcatcgtg tccggcttga
     1681 tgtcgacgaa caccgggatg ccgttgtggt gcaggatgca ggtggccgtc gaggtccagg
     1741 agaacgccgt ggtgatcacc tcatcccccg gctggatccc cacgcccgcc accgccatgt
     1801 ggagggccgc ggtcccgctg ttggtggcca ggcagtactt cgtcccgatg taccgggccc
     1861 actcctcctg gagcttcgtc acctcgggag ccgtgccgcc ccacaggatt ccgcggtcca
     1921 ggacgcgcat gaccgcgtcc ttgtcctcct gccggatctc tggccagggc ttgatgaacc
     1981 cgtccgggac cgccttcctg ccgccccgga tggccagcgc actcttctgt tttgtggcca
     2041 tgacctcccc tttcatcctt tcgccgcaac ggcggacagc cccggatcag gcctctacct
     2101 cggcagcgcc tcgcggaaga agcgaagcca gttgccgtgc atgacggctt ccacgtccgc
     2161 gtcgccataa ccgcggcggc gcagccggtc gggcacctgc tggaggtcgg cgatcgtgtc
     2221 cagctctcgc ggtgactgct ccgttccgaa gccgccgtcc aggtcgctgc cgatcgccgc
     2281 gtgccgggca ttccccgcta tctggcacac gtggtcgatg tggtcgatta ccctctccag
     2341 gctcaccagg gagttgtcgg tctccccgcg cgcgtagccg gggtacagca tccaggagtc
     2401 gaaggccgct ccgatgacgc cgccgcgggc aaccagtgcc cggatctgat cgtcggatag
     2461 ctgacggtcg cccggcacga gcgctcggca gttgtggtgg ctggccatca ccggtccatg
     2521 gaagagatcg agcgactccc agaagctccc gtccgccatg tgagtgatat cgaggatcat
     2581 gcccgcggac tccatggcgg caagaaggtc gcgtcctgga ggcgtgagcc cgcccggtgc
     2641 gccggtgccg tgagcgtaaa agctgatgcc gtagtgcacc ggctccacca cgcgcagacc
     2701 gcgctcccac cagcgagcga cgtcgttcgg ccgaaggatg gggtcagccc cttccatcgc
     2761 caggatgaac ccgaggggtg tcccggcgcc accggtccgt tgccattcgg accagtgggc
     2821 atccagggac gccgcgtccc gcaccatgcg caggtgccct gccgcctcca tgatccggta
     2881 gtactccacg tgcgccgcac cctcggcggc ggctatctcc cgggtgcggc ggtccaggaa
     2941 cggatcatcc agggtgctgc atcgggacag caccgtggcc aggcagacgg cgacttcggc
     3001 gcggtgcagc tcggggaagg agacggtgtt gtggccccgg cggaggtcgg agagccccac
     3061 ctccgaccgc cggatctcct ccacggtcgc ggtaaggtcc cggttccagg cgagagcgca
     3121 ccaggccaga tccaggtggg aatcgatgac ggtcatcggc gctcgcgtcc cctcgcgtgg
     3181 ctcgcggccg gcttcggcta cttgaagctc accgtgtagt ccaggggctt gtccagcacc
     3241 aggagccgca ggagggggat cttgaacacc acggcgcccc cg
//
LOCUS       DASRZS010000456         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1781253, whole genome shotgun sequence.
ACCESSION   DASRZS010000456 DASRZS010000000
VERSION     DASRZS010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781253"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFH83_11160"
     CDS             <1..318
                     /locus_tag="VFH83_11160"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6486971.1"
                     /translation="AWVSKSLQDPMFHKTRSARIGIIQRDANEWIRAQTCFPVPLLLM
                     QGTGDRYVDPQATIAFARNLAGDVTLKVWDGLGHELHNELPRAEVLAFVRAWLDERVG
                     RGR"
     gene            426..1124
                     /locus_tag="VFH83_11165"
     CDS             426..1124
                     /locus_tag="VFH83_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486972.1"
                     /translation="MRVQSAMEFLSRVAVRVATNATWVLAIVIFLSLLSSAFSIRYLS
                     SLQGDIDEIYENEVRGQTYAQNAYVALVEIESEVKDLVLSESEDYRLASATDITQHCT
                     ALQALVRRATLTMSATRYRTLISNVTKDVSSVADTIRHRLAGGTPTQEQARALLTELE
                     PLASALRTDIATINDVKRTGNTSGLRAIRIQLQVSLATTVTILLLSITVRLLIYRSSR
                     PAAASARRRIGKQQ"
     gene            1179..1745
                     /locus_tag="VFH83_11170"
     CDS             1179..1745
                     /locus_tag="VFH83_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486973.1"
                     /translation="MGPAPAQRMALLVIMTAALFVSARAEADLKAVFAFADRLVSVRF
                     PELNYWILTTVDQNTRRIAVTFPEPAGHRETDPPQPHLEIVLEHVGPAVGQTDYHQHY
                     LDEYAQSMGASPAGPDESPPSGMGLTDCRMTLLQFTDAELNAHTALLITAVDGDVGIV
                     ALLDARAETFEKNKDVSSAIVRSIRFED"
     gene            1785..2147
                     /locus_tag="VFH83_11175"
     CDS             1785..2147
                     /locus_tag="VFH83_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006038532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET6486974.1"
                     /translation="MESRSEEIDSYILGQPPEVRRKLTTLRQLIRRAAPEAKEKISYG
                     MPTFWLNGNLVHFAARARHIGLYPTSSPITKFKRELARYVTSKGAIQFPLEEPLPLEL
                     IRKIVRFRVEQNRGKSAS"
     gene            2144..2605
                     /locus_tag="VFH83_11180"
     CDS             2144..2605
                     /locus_tag="VFH83_11180"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6486975.1"
                     /translation="MIRAGTEGDAPLILAVILRAFEEYRGTLDPPSGAHNETRESISE
                     KIGQGGAAVALAGSKVVGCALWKEEPGYLYLGRLAVLPEFRRASIGERLVEWIEEEAA
                     RLGAPKVQLKVRSALGATREYYERRGYRVTGQGSHPGYAGPTYVVMEKVLR"
BASE COUNT          452 a          897 c          851 g          419 t
ORIGIN      
        1 gcctgggtgt cgaagagcct ccaggacccg atgtttcaca agacccggtc cgcccgcatc
       61 gggatcatcc agcgtgacgc gaacgagtgg atacgggccc agacgtgctt cccggtaccg
      121 ctcctgctca tgcagggaac cggggaccgc tacgtggacc cgcaggctac catcgcgttc
      181 gccaggaacc tggcgggaga cgtgacgctg aaggtgtggg acggcctggg ccatgagctg
      241 cacaacgagc tgccccgggc ggaagtgctc gcgtttgtcc gagcctggct ggacgagcgc
      301 gtgggaaggg gacggtagcg gcgggatccc cacgtgcggc tccgcggaag aatggcgctc
      361 acgcccctgc ttgccagcgt ccgccccgtc ggcccagact gggtttcgga atggagggcc
      421 gaagcgtgag ggtccaatcg gccatggagt ttctttcccg cgtggcggtc cgtgtcgcca
      481 cgaatgccac gtgggtgctc gccatcgtca tcttcctgtc cctgctgtcc agcgcgttct
      541 cgatccgcta cctcagcagc ctccagggcg acatcgacga gatctacgag aacgaggtgc
      601 ggggccagac ctacgcccag aacgcgtacg tagccctggt ggagatcgag tccgaggtga
      661 aggacctggt gctctcggag agtgaggact accgcctggc ttccgccacg gacatcaccc
      721 agcactgcac ggccctgcag gcgctcgtcc ggagggccac gctcacgatg agcgccacga
      781 ggtaccgcac gctcatctcg aacgtcacga aggacgtctc cagcgtggcc gacacgatac
      841 gccaccggct cgcgggcggg actcccacgc aggagcaggc tcgcgcgctg ctcacggagc
      901 tggagccgct tgcctccgcc ctccggaccg acatcgccac catcaacgac gtgaagcgca
      961 ccggcaacac gagcggactc cgggccatcc gcatccagct ccaggtctcc ctggccacta
     1021 cggtgaccat ccttctcctt tcgatcaccg tgaggctgct catctaccgc tcttcgcggc
     1081 ccgccgcggc ctcggcccgc cggaggatcg gaaagcaaca gtagtcaccg cgcgggaatt
     1141 gtcccgcccc gcccgcctca gtaccttctg acccggatat gggtccggca ccagcgcaac
     1201 gcatggctct cctggtcatc atgaccgctg ccctgttcgt cagtgcgcgg gccgaagccg
     1261 acctgaaggc cgtgttcgcg ttcgcggacc ggttggtctc ggtccgcttc cccgagctca
     1321 actactggat cctgacgacc gtggaccaga acacgcgccg catcgcggtg acgtttcccg
     1381 agccggccgg ccaccgggaa accgacccgc cgcaacccca cctggagatc gtgctggagc
     1441 acgtgggacc cgccgtcgga cagacggact accaccagca ctacctggat gaatacgcgc
     1501 agtccatggg ggcgagcccc gcggggcccg acgagtcccc tccctcgggc atgggcctga
     1561 cagactgccg catgacgctc ctccagttca ctgacgccga actgaacgcg cacacggcgc
     1621 tgctcatcac cgccgtggac ggggacgtgg ggatcgtggc cctcctggac gcccgggccg
     1681 aaacctttga gaagaacaag gatgtctcct ccgccatcgt tcggagcatc cggttcgagg
     1741 actaggctgg cagttttccc ggtcctcgac tattgtaggc ttcgatggaa agccgctcgg
     1801 aagagataga cagctacatc ctcggtcagc ccccggaggt gcgcaggaag cttacgaccc
     1861 tccggcagct catccgccgc gccgcgccgg aggccaagga gaagatctcc tacgggatgc
     1921 ccacgttctg gctgaacggc aacctggtcc attttgccgc tcgcgcccgg cacatcgggc
     1981 tgtaccccac ctccagtccc atcacgaagt tcaagcggga gctcgcccgg tacgtgacgt
     2041 caaagggagc catccagttc ccgctggagg agccccttcc cctggagctg atacggaaga
     2101 tcgtccgctt ccgcgtggaa caaaaccgag ggaagagtgc gtcgtgatcc gcgccgggac
     2161 ggaaggggat gcgccgctca tcctggcggt gatcctgcgg gcattcgagg agtatcgcgg
     2221 cacgctcgac cctccgtccg gcgcccacaa cgagacgcgg gagtcgatca gtgagaagat
     2281 cggccagggg ggcgctgccg tggcccttgc cgggtcaaag gtcgtgggct gcgcgctgtg
     2341 gaaagaggag cccggctatc tctacctggg ccggctggcc gtccttcccg agttccgccg
     2401 agcgagcatt ggcgagcgcc tggtggaatg gatcgaggag gaggccgcgc ggctgggcgc
     2461 tccgaaggtg cagctcaagg tgcgctccgc cctcggtgcc acgcgggagt actacgagcg
     2521 ccgcgggtat cgggtcaccg gccaggggtc tcatccgggc tatgccggtc ccacctacgt
     2581 cgtcatggag aaggtgctcc gatgagctcg gccgtcatc
//
LOCUS       DASRZS010000457         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1018351, whole genome shotgun sequence.
ACCESSION   DASRZS010000457 DASRZS010000000
VERSION     DASRZS010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018351"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VFH83_11185"
     CDS             complement(<1..837)
                     /locus_tag="VFH83_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6486976.1"
                     /translation="MKTIRISFLDITHPHVWTRADIFREMPGVTLQSVWEPKDVKGAA
                     TFAERYGVRRAESAESVLRDPRVDAVVVESFTQNMADIAVAALQAGKPVLLEKPAANN
                     PANMQRIVDAARTSRKLLQVGYMMRQAAMVDFARDILAGAVLGRVTAARFHVSVPAPD
                     AVTPWFNLADDIGGVLFEDGCHMMDIALNLMGRPRRVSAFVSKFGDLTRKHRHMYEDA
                     AMVNLDWGDCLGTFSMIGWEANEWIETWEMEFYGTEGTLQVGLLPATSRLFLKTPKAG
                     YPA"
     gene            complement(842..1816)
                     /locus_tag="VFH83_11190"
     CDS             complement(842..1816)
                     /locus_tag="VFH83_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6486977.1"
                     /translation="MPSAQPAPERAPAGFRRVFDVQQWGIVFVLIALVIIISVAAPVF
                     LSPRNIRNVLQQVSTLGILSMGVTVLMISGGIDLSVGSAMSTVAVIAGTLLKAGAPVE
                     VAVIAGLILGCAIGCLNGMLAAFTKAHPFILTLGTMTILQGVAIIVTQGYPINDLGAR
                     FAYIGGAMFGPIPFIVILFFLVMIVCGLLLRFLRVGRIAYAIGGNEYTTFLAGIPVKR
                     YKILFYVICGFFAGMAGLVLASRISSAMPSLGTGFEMNSIGAAVIGGIPLTGGRGNVW
                     GTLTGVLLLGIINNGLNLLHVDASWQYVVTGLIIIIAVIIHERRLKKR"
     gene            complement(1791..>2328)
                     /locus_tag="VFH83_11195"
     CDS             complement(1791..>2328)
                     /locus_tag="VFH83_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6486978.1"
                     /translation="RPLPEFIRRGICFVPGERDTRGLVLSMSIAANTTLAHLDGVTHG
                     PLMDLPRERQVAEEYVRTLAVQARSVTQEVQYLSGGNRQKVMLAKWLYLGLEVFLLDE
                     PTQGVDVGAREEIHRIMKRLLDDGKGILMVSSDLDELMNMSHLIAVMNKGRLAAVLEA
                     EKTNRQEVLSYAIGSTGA"
BASE COUNT          423 a          785 c          726 g          394 t
ORIGIN      
        1 ggcggggtag ccggccttcg gggtcttgag gaagagacgg gaggtggcgg gaagcagacc
       61 cacctgcagg gttccctcgg tgccgtagaa ctccatctcc caggtctcga tccactcgtt
      121 ggcttcccag ccgatcatgg agaaggtgcc cagacagtcg ccccagtcga ggttcaccat
      181 ggcggcgtcc tcgtacatgt gcctgtgttt ccgcgtgagg tccccgaact tggacacgaa
      241 tgcggagacc ctcctgggcc tccccatgag attcagcgcg atgtccatca tgtgacagcc
      301 gtcctcgaac aggacgccgc cgatgtcgtc cgccaggttg aaccacgggg tcacggcgtc
      361 cggggcggga accgagacgt gaaaccgcgc cgcggtgacc cgcccgagaa cggcgcccgc
      421 aaggatgtcg cgggcgaagt ccaccatggc ggcctgccgc atcatgtacc cgacctgcag
      481 gagcttcctg ctcgtccgcg cggcgtccac gatccgctgc atgttcgccg ggttgttcgc
      541 agccggcttt tccagcagca ccggcttgcc cgcctgaagc gccgccaccg cgatgtccgc
      601 catgttctgc gtgaacgatt ccacgaccac cgcgtccacc cgtgggtcgc gcaggacgga
      661 ctccgcggac tccgcgcgcc tcaccccgta tcgctccgcg aaggttgccg cgcccttgac
      721 gtctttcggt tcccagacgc tctgcagcgt cacgcccggc atctcgcgga agatgtccgc
      781 gcgcgtccag acgtgcgggt gggtgatgtc gaggaacgag atccggatcg tcttcatgag
      841 cctacctctt tttcagccgc ctctcgtgga tgatgaccgc gatgatgatg atgagccccg
      901 tcaccacgta ttgccagctg gcatccacgt ggagcaggtt caggccattg ttgatgatgc
      961 ccaggaggag gacacccgtc agcgttcccc acacgttgcc gcggccgccg gtgagcggaa
     1021 tccctccaat gaccgccgcc ccgatggagt tcatctcgaa ccccgttccg aggctgggca
     1081 ttgccgagct gatccgcgag gccagcacga gaccggccat gccggcgaaa aacccgcaga
     1141 tcacgtagaa gaggatcttg taccgcttca cggggatgcc cgccaggaac gtggtgtact
     1201 cgttcccgcc gatggcatag gcgatacggc ccacacgcag gaaccgcagg agaaggccgc
     1261 agacgatcat cacaaggaaa aacaggatca caatgaacgg gatgggtccg aacatggccc
     1321 cgccgatgta cgcaaaccgg gcccccaggt cgttgatggg gtatccctgg gtcacgatga
     1381 tggccacgcc ctggaggatc gtcatggtcc ccagcgtcag gatgaagggg tgggccttcg
     1441 tgaacgccgc cagcatcccg ttcaggcacc ctatggcgca gccgaggatg agaccggcaa
     1501 tgacggccac ctccaccggt gcccccgcct tcagcagggt ccccgcgatg acggcaaccg
     1561 tcgacatggc ggagcccacc gagagatcga tcccgcccga gatcatcagg accgtgacgc
     1621 ccatggacag gatgccgagg gtggagacct gctgcagcac gttgcggatg ttccgcggtg
     1681 acaggaacac gggggcggca acgctgatga tgatgaccag cgcgatgagg acgaagacga
     1741 tgccccactg ctggacgtcg aaaacccgcc ggaaccccgc gggggcgcgc tcaggcgccg
     1801 gttgagccga tggcatacga gagaacctcc tgtctgttcg tcttctccgc ttcgaggacg
     1861 gcggccagcc gtcccttgtt catcaccgca atgaggtgac tcatgttcat gagctcgtcg
     1921 aggtccgacg acaccatgag gatgcccttg ccgtcatcca gcagcctctt catgatcctg
     1981 tgaatctctt cccgcgcccc gacgtccacg ccctgcgtgg gctcgtccag gagaaacacc
     2041 tccaggccca ggtacagcca cttggcaagc atcaccttct gcctgttgcc gccgctcaag
     2101 tactgcactt cctgtgttac gctgcgcgcc tgcaccgcga gggtccgcac gtactcctcg
     2161 gccacctggc gctcgcgcgg aagatccatg agcggcccgt gcgtcacgcc gtccaggtgg
     2221 gcaagggtgg tgtttgccgc gatgctcatc gagaggacca gcccccgcgt gtccctttcc
     2281 cccgggacga agcagatccc gcggcggatg aactccggaa gcggcctg
//
LOCUS       DASRZS010000458         5293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1403407, whole genome shotgun sequence.
ACCESSION   DASRZS010000458 DASRZS010000000
VERSION     DASRZS010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403407"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="VFH83_11200"
     CDS             <1..1356
                     /locus_tag="VFH83_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6486979.1"
                     /translation="VLIAAGLSALLWDYLFLPPRFTFLITKLEDVLMFFLYFVTAAAL
                     AILMSRLRANQRMLAVRARRMTLLLDFSQALSVQRALEGIVRVGTEHISRYLEAEAIL
                     FLKDIYGGLGRTARTSKAQSTQEVDEREYAAARWSFDNRTPCGRYTDTLHLAYYHYIP
                     LLTPDSAVGVLGIRLPEGRSWLKDQEDSLQLLGRTLALSIERELLAEENRRNLMARES
                     ERLGRVLLNSVSHELRTPLTTIKGSVTALMDDSTVSDPQARGILLAETLTAADRLNAI
                     VENLLSMSRLESGMLKLKRSTNDVFDLVSVVADALRRQSKDHPLSIQIDAEVPAVSLD
                     FVLMVQVLTNILLNAARHTPAGTPVEVRVEREGQGILMTVADEGPGVPPEELPHLFES
                     FFRGRKAATGGVGLGLSICKGIVEAHGGTISAYLNRKGGLSIAIHLPDSVVEEPREDA
                     S"
     gene            1391..2107
                     /locus_tag="VFH83_11205"
     CDS             1391..2107
                     /locus_tag="VFH83_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6486980.1"
                     /translation="MTSVLVIDDEAQIRRLLRVSLEKQGFAVHEAGTGSDGLQMVLGK
                     KPDIVLLDLGLPDRDGAQVLQELRGWSTLPVIVLSVRNTEEDIVRLLDAGADDYLIKP
                     FNTGELIARLKVAIRHHAPAEGQETFASGHLEVDLLHREVKVRGEPVKLTPTEYGLLR
                     LFVQHAGRVLTHRQIMREVWGPNAEAETNYLRVYVTGLRKKIEDRPQMPELLITEPGV
                     GYRLRVLASEGVARSPDAAP"
     gene            complement(2104..3624)
                     /locus_tag="VFH83_11210"
     CDS             complement(2104..3624)
                     /locus_tag="VFH83_11210"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET6486981.1"
                     /translation="MTCLLGIDVGTYESKGVLVDSGGRILASAARPHPMAIPRPGWAE
                     HDAETVWWADFVSLARELPKLAGVSGPQIAAVSTSGIAPCVLPVDAAGTPLRPAILYG
                     VDTRASAEGKELTAALGEEWILSQTGSALSSQAAGPKILWIRRHEPDVWRRTARIHTS
                     TSYLVHRLTGRSVMDHYTAAAYGPLYNLRARGWDPRALSLVCEASLLPELDWSAAVAG
                     RVSPAAAAETGLAEGTVVAVGTADAASEAVAAGVLDPGDCMLMYGSTLFFVQICSGLP
                     SSRTQWPTVYLEPGTYALAAGMSTTGALTRWFRDTFAREELAAEQGGGVNAYQALSDA
                     ASRVPPGSQGLMVLPHFSGERTPINDPEARGVIVGLTLSHGRPEIFRAILEGIAFGIR
                     HNIEAMAEAGAAPRRLVAIGGGVRNRLWVQIVSNATGLPQTVQNTPGACYGDAVLAAI
                     SVGQLQRMSQARRWLPSGTEVRPDPAATGVYDRLYRIYRELYDGTKAVSHRLASIF"
     gene            complement(3668..4051)
                     /locus_tag="VFH83_11215"
     CDS             complement(3668..4051)
                     /locus_tag="VFH83_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HET6486982.1"
                     /translation="MSRYQDPDAVEATGTFHCIYCQKLVTPAASGTEQRNHCPYCLWS
                     RHVDFRTGDRRSACRSAMEPIAVWAKPNGEWSVVHRCKSCGALRDNRIAGDDDELILL
                     SVALKPLARPAFPLDRVARLGATPS"
     gene            4371..>5293
                     /locus_tag="VFH83_11220"
     CDS             4371..>5293
                     /locus_tag="VFH83_11220"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HET6486983.1"
                     /translation="MKTQVTVLALAVAGMTLLASGLWADPPSEVARLNLISGSVSFQS
                     GSLDDWAPAVLNYPLTTGDHLWSDAGGRAELHVGSSALRLGPGTDFTFLGLDDQTVQV
                     RLADGSLDVRVRDLDPGVTFEVDTPNASVQLTRDGSYRIDVRDSGDTVVVARAGAADV
                     TAGGAGFDVAGGQSAVITGAGSVGYYVTGIPAQDDWDRWCLARDQREDSIASVRYVPR
                     AMIGVEDLDANGSWSTVAGYGPVWTPRVAVGWAPYRFGHWSWVEPWGWTWVDDAPWGF
                     APFHYGRWAFLQGRWVWIPGTIVARPVYAPA"
BASE COUNT          837 a         1768 c         1838 g          850 t
ORIGIN      
        1 gtcctcatcg cggcaggcct gagcgcgctc ctctgggact acctcttcct tccgccgcgg
       61 ttcactttcc tgatcacaaa gttggaagac gtcctgatgt tcttcctcta cttcgtgacc
      121 gccgcggcgc tggctatcct catgtcgcgg ctccgggcca accagcggat gctggcggta
      181 cgagcgcgga ggatgacgct cctcctggat ttctcccagg cgctctcggt ccagcgcgcc
      241 ctggaaggga tcgtacgggt cggaacggag cacatttcac ggtacctgga ggcggaggcg
      301 atcctcttct tgaaggacat ctacggcggt ctggggcgga cggcacgcac gtccaaggcg
      361 cagagtacgc aggaggtgga cgagcgggag tacgcggcgg cccgctggag cttcgacaac
      421 cgaaccccgt gcggccggta cacggatacc ctgcacctgg cctattacca ctacattccc
      481 ctcctgacgc cggactccgc ggtcggggtg ttggggatcc gtctccccga ggggaggagc
      541 tggctcaagg accaggagga ctcgctccag ctcctggggc gcaccctggc cctctccatc
      601 gagcgggagc tgctggcgga ggagaaccgc aggaacctca tggcgcggga gtcggagcgg
      661 ctgggacgcg tgctcctgaa ctccgtttcc cacgagcttc ggactcccct gaccacgatc
      721 aaaggctcgg tgaccgccct catggacgac agcaccgtat ccgaccctca ggcccgcggg
      781 atcctcctgg ccgagacgtt gaccgcggcg gaccggctga acgccatcgt ggagaacctg
      841 ctcagcatga gccggctcga gtccggtatg ctcaagctca agcgctccac caacgatgtc
      901 ttcgacctgg tctccgtcgt ggcggatgcc ctgcgtcggc agtccaagga ccatcccctg
      961 tccatccaga tcgacgcgga ggtgcccgcc gtctccctgg acttcgtgct catggtccag
     1021 gtgctcacca acatcctcct gaatgcggcc cgtcacacac cggccgggac ccccgtggag
     1081 gtccgggtgg agcgggaggg tcagggcatc ctcatgaccg ttgccgacga ggggccgggc
     1141 gtcccgccgg aggagctgcc gcacctgttc gagtccttct tccgcggccg caaggcggcg
     1201 accggcggcg tgggtctggg gctctcgatc tgcaagggga tcgtggaagc ccacggtggg
     1261 acgatctccg cgtatctgaa ccgcaaggga ggtctctcca tcgccatcca cctgcccgac
     1321 agcgtggtgg aggagccacg ggaggacgcg tcgtgacagg cgcggcggcc agggcgtcgg
     1381 agcgtgcgtc gtgacgagcg tcctggtcat cgacgacgag gcgcagatcc gccggctcct
     1441 gagggtcagc ctcgagaagc aagggttcgc ggtccacgag gcgggcacgg gcagcgacgg
     1501 gctgcagatg gtgctgggga agaagcccga cattgtgctc ctggacctgg ggctgccgga
     1561 ccgcgacggg gcgcaggtgc tccaggagct tcgcggatgg tccaccctgc ccgtgatcgt
     1621 cctctcggtc cgcaacacgg aggaggacat cgtgcgcctc ctggacgcgg gcgcggatga
     1681 ctacctgatc aagccgttca acaccggaga gctgatcgcc cgtctcaagg tggccatccg
     1741 gcatcacgct cccgccgaag gccaggagac gttcgcctcc ggtcacctcg aggtggatct
     1801 gctgcaccgc gaggtgaagg tccgcggaga gccggtgaag cttacgccca cggagtacgg
     1861 tctcctccgt ctcttcgtcc agcatgcggg ccgggtcctg acgcaccggc agatcatgcg
     1921 ggaggtgtgg ggacccaacg cggaggcaga gaccaactac ctgcgggtct acgtgaccgg
     1981 gctgcgcaag aagatcgagg accggccgca gatgcccgag ctgctgatca ccgagccggg
     2041 ggtcgggtac cggcttcggg tcctggcctc cgaaggcgtg gcaaggtcgc cggacgccgc
     2101 cccctagaag atcgaagcca ggcggtggga gacggccttg gtgccgtcgt acagctcccg
     2161 gtagatcctg tagagccgat cgtagacgcc ggtggccgcg gggtccgggc gcacctcggt
     2221 ccccgacggg agccagcgcc ttgcctggct catacgctgg agctgcccca cggagatggc
     2281 ggccaggacg gcgtccccgt agcaggcgcc cggggtgttc tgcacggttt gcgggagacc
     2341 ggtcgcgttg ctgacgatct gcacccacag ccggttgcgt accccgccgc cgatggcaac
     2401 cagcctgcgc ggcgctgcac ccgcttccgc catcgcctcg atattgtgtc ggattccgaa
     2461 cgcgatgccc tccaggatgg cgcggaagat ctctggccga ccgtgggaga gcgtcagccc
     2521 gacgatcacc ccgcgggctt ccgggtcgtt gatcggcgtg cgttccccgc tgaagtgcgg
     2581 aaggaccatc aggccttgcg agcccggggg aacccgggag gcagcgtccg acaaggcctg
     2641 ataggcgttg accccgccgc cctgctccgc cgccagctcc tctcgggcga acgtatcccg
     2701 aaaccaccgc gtgagcgctc cggtggtaga catgcccgcg gccagggcgt acgtgccggg
     2761 ttccaggtag accgtgggcc actgggtcct gctggacgga agccccgagc agatctggac
     2821 gaagaagagc gtgctcccgt acatcagcat gcagtcgccg gggtccagga cgccggcggc
     2881 cacggcctcc gaggcggcat ccgcggtgcc caccgcgacc acggtcccct cggccagccc
     2941 ggtctcggcc gcggcagccg ggctcactct gcctgcgacc gccgcgctcc agtcgagctc
     3001 cggcagaagc gacgcctcgc agacgagact cagcgcgcgg gggtcccagc cgcgggcccg
     3061 aaggttgtac agcgggccgt aagcggctgc cgtgtagtgg tccatcaccg accgtcccgt
     3121 cagtcggtgg accaagtagg acgtggacgt gtggatcctg gccgtgcgcc gccacacgtc
     3181 cggctcgtgg cgacggatcc agaggatctt gggaccggcc gcctgggagc tgagagcgga
     3241 cccggtctgg gagaggatcc actcctcccc gagggcggcg gtcagctcct tcccttcggc
     3301 ggaggcgcga gtatccaccc cgtagaggat ggccggccga agcggcgtac cggcggcatc
     3361 gacggggagg acgcagggag cgatgccgct ggtgctgacc gcggcgatct gcgggccgga
     3421 cacgcccgca agcttcggca gctcgcgcgc cagcgagacg aagtccgccc accacacggt
     3481 ctcggcatca tgctccgccc agcccggcct ggggattgcc atagggtggg gccttgcggc
     3541 gctggccaga atgcgccccc cggaatccac cagcacgccc ttggattcgt aggtaccgac
     3601 gtcgatgccc agcaggcagg tcatcccgcc attgtgtgcc tccggatcgg gcgggcgtca
     3661 acggcggtca cgaaggggtc gcccccagcc gggctacgcg gtccagcggg aacgccggac
     3721 gggccaatgg cttcagcgcg acggacagga ggatcagctc gtcatcatca ccggcaatcc
     3781 ggttgtcccg cagagccccg cagctcttgc atcgatggac gacggaccac tcgccgttgg
     3841 gcttcgccca gaccgcgatc ggctccatgg ccgaccggca ggcggagcgc cggtccccgg
     3901 tccgaaagtc cacgtgccga ctccacagac agtacgggca gtggttgcgc tgctccgttc
     3961 ccgacgcggc gggggtgacg agtttctggc agtagatgca gtggaaggtg ccggtcgcct
     4021 caacggcgtc cgggtcctgg tatctcgaca aggatcaatt ccttcatctg gagaggccat
     4081 gcgcgcctcg cgaaggaatc gaccgacttc acacgctcaa tcgaacggtc cgattacctc
     4141 cgcgagctcc caagagcaga cccgttccgc tgcgcaaccc tcacaagagc cgccatccgt
     4201 ccctcctatc agcaaggttt tgttaccgaa cttccgaccg ccaacggtgt atctttttct
     4261 tgtcgttgtc aacggggccg cctgccccgg tgcgcccatc accggtcgag ctggctcgga
     4321 ccggtaaccg tcccggtggt ggcggtgttt caatgacgga gaggatttcg atgaaaaccc
     4381 aagtcacggt tctggccctg gcggttgcgg ggatgacgct cctggcgtcc ggtctctggg
     4441 cggatccgcc atccgaggtg gcccggctca acctgatcag cgggtcggtg tctttccagt
     4501 cgggaagcct ggacgattgg gcccccgcgg tcctcaacta tcccctcacc acgggagacc
     4561 acctctggtc ggacgcgggg ggacgcgctg agctccacgt ggggtcatcc gcgctccgtc
     4621 tcgggccggg caccgatttc accttcctgg gtctggacga tcagacggtg caggtccgcc
     4681 tggcggacgg ctccctcgat gtccgggtga gagatctgga tcccggggtc accttcgagg
     4741 tggacacccc caacgccagc gtacagctta ctcgggacgg cagctatcgg atcgacgtgc
     4801 gggacagcgg agacaccgtc gtggtggcgc gagcgggcgc ggcggacgtc acggccggcg
     4861 gggccggatt cgacgtagcc ggcgggcagt ccgccgtgat caccggcgcg ggaagcgtcg
     4921 ggtattacgt gaccggaatc ccggcgcagg acgattggga ccgctggtgt ctggcccggg
     4981 atcagcggga ggacagcatc gcctcggtcc gctacgtgcc ccgggccatg attggcgtgg
     5041 aagacctgga cgcgaacgga tcctggagca cggtggccgg atacgggccg gtgtggacgc
     5101 cgagggtcgc cgtgggctgg gcaccctacc ggttcggcca ctggtcgtgg gtcgagccct
     5161 ggggatggac gtgggtggat gacgcgccat ggggctttgc tcccttccac tatgggcgat
     5221 gggcattcct ccaggggaga tgggtctgga taccgggcac catcgtggcg cgccccgtgt
     5281 atgcgccggc gtt
//
LOCUS       DASRZS010000459         5740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1403420, whole genome shotgun sequence.
ACCESSION   DASRZS010000459 DASRZS010000000
VERSION     DASRZS010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403420"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VFH83_11225"
     CDS             complement(<1..962)
                     /locus_tag="VFH83_11225"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HET6486984.1"
                     /translation="MAPDSNPLQAYTRARHAIADVLQELQANLSIEPSDPRDAERCRS
                     LLAKLAEDRFNLVVVGQFSRGKSTLMNALFGRPVLPTGVLPLTSIITSVRYGHRETLL
                     LRRQDSMFLTEVPIERMADWVTQQGNPGNRKRLSEAIVELPLPFLRRGFYFIDTPGIG
                     SAETANTATTLEFLPQVDAVIFVTSVDSPMTETEVGLLRQTARFTDEVFLVLNKLDLL
                     REGDARDASTVAGFVQEHARRVLGSTPRIYLVSALKALEAAAAGDEDALSRSGLARLR
                     ADIERLLSEKREAVFLASMTTKTLEVITDLESRASLFARSARMG"
     regulatory      complement(1077..1136)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(1207..2499)
                     /locus_tag="VFH83_11230"
     CDS             complement(1207..2499)
                     /locus_tag="VFH83_11230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6486985.1"
                     /translation="MSREERASPLGPLWGTAFLASLPFAVLAFALPIYGKMLGASALQ
                     VGALFSAVSFAPVLVRPFLGRMVDRWGRRPFILAGLASYAVSMALTAMAGSVALLAVA
                     RVVQGLGQAFFWLSAYTIVADVAHEWDRARGFGAIDEASSRGSLVGALPGVALIAVTQ
                     ATGLPWAAVWPWLFGVYTLGAGAGLALGAWRIGETGPSRGGSSRLPSHESDPSYHAPR
                     RLSLQLIALMGIVFITGASTAMVWPVLVVFLEDRLKAGAFELAVAYLPAALVSSFLPS
                     RIGRLTDRMARKPLMVAGLVTAGVASAVMPRLRAVWPLAILWAVDSLGYAASSPAERA
                     FVADIAGRDRRGRSYGLYTFAYFLGAAVGPLAGGWLYDTMGQSYPFYGNSIVLGLAAA
                     LLIALLREPARSRAVRRPPGGRGSVPPAHRRADGPGMR"
     gene            2682..3464
                     /locus_tag="VFH83_11235"
     CDS             2682..3464
                     /locus_tag="VFH83_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6486986.1"
                     /translation="MPAWTRTLLLFLANIALLAAGILLYDALKPKPQHLTQADIDSAV
                     LRTLDALPPKPSDASVAYNVIRPSLVSVVARIGTGDKAKTSLGSGVVVVDTGMILTAL
                     HVVDGASDVQVTFADGSQSGALVVVRQPEHDLAVLSAITAPDDLKPATLVSSGTLQPG
                     DEVIAVGDPFGIVNSVSAGVVSGLHREYTDRKGNVTLKDLIQFDAAVNPGNSGGPLLN
                     RNGEVVGIVDSLLNPTAQEVFIGIGFAVPIETASAALGFSPM"
     gene            3513..4619
                     /locus_tag="VFH83_11240"
     CDS             3513..4619
                     /locus_tag="VFH83_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6486987.1"
                     /translation="MESSSEHGGSPVSAQSPTAPSAAEGGAQGTPAGRLPEEAVAEQA
                     LLMEQILYEVKKVIVGQDHLLERVLVALLSQGHLLVEGVPGLAKTLTVKTVADAVQGI
                     FKRIQFTPDLVPADLVGTRIYNQKTGDFSTSLGPVFANLLLADEINRAPAKVQSALLE
                     VMQERQVTIGGQTFKVPEPFLVMATQNPIETDGTYPLPEAQVDRFMMKTLVDYPSEAE
                     EFVIVERVTGTMAPVKPVVTTQQIVELRGWCERVFVDPILVQHAVRLASATRAPDQHG
                     VPDIAKYIVYGASPRASINLIIGARALAFLRGRTYVVPQDLTDMVLDVMRHRIIPSYE
                     ALSDGVTTDDILAKVLEAIPAPPQALKTHAASGG"
     gene            4600..5544
                     /locus_tag="VFH83_11245"
     CDS             4600..5544
                     /locus_tag="VFH83_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6486988.1"
                     /translation="MLPPAGDPERILQRLEWTVIRRLDGLLQGNYRTLFRGFGTELAE
                     LREYVLSDDVRYIDWYVTARLQTPYVRQYVEDREITAWFLLDLSPSLDFGTVTTEKRT
                     LLADCVGVLARLLTRHGNRVGALFFAGSQERFIPAQNGRNHVLRILEDLLRQPRLARA
                     PMTDLSFPLTTALRMIRRRSLIFLVSDFISAPGWEKPLSLLTQRHELLAVRVTDPRES
                     ELPDIGPLVLEDSETGEQLFVDTHDRAFRRRFADAAASREASLQAMLARRGIDLLSLS
                     TEGDLVAELMRFAQIRRERKKLPSRQNLPRGAAMAAAG"
     gene            5561..>5740
                     /locus_tag="VFH83_11250"
     CDS             5561..>5740
                     /locus_tag="VFH83_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HET6486989.1"
                     /translation="MSFQWSSLLWFLILVPILVLLYLWAQRRRQRYALRYASLSLVKE
                     AVGRGPGIRRHIPAIL"
BASE COUNT          960 a         1962 c         1927 g          891 t
ORIGIN      
        1 tcgcccattc gggccgaccg ggcaaagaga gaagctcgcg attcaagatc cgtgatcacc
       61 tcaagggtct tggtggtcat cgaagccagg aagacggctt ccctcttttc ggacaggagt
      121 cgctcgatgt ccgccctcaa ccgagccagc ccgctcctgc tcaacgcgtc ttcgtcgccg
      181 gctgccgctg cctcgagggc cttcagcgcg gagaccagat agatccgggg tgtggaaccg
      241 aggactcgcc gggcatgctc ctgcacgaag ccagccacgg tcgacgcgtc gcgcgcatct
      301 ccttcccgca gaagatccaa cttgttgagg accagaaaga cctcgtccgt gaaccgcgcc
      361 gtctgcctca ggagccccac ctcggtctcg gtcatcggcg aatcgacgct cgtgacgaag
      421 atcaccgcat cgacctgcgg caggaactcg agggttgtgg cggtgttcgc ggtctcggcg
      481 gatccgatgc cgggcgtgtc gatgaagtag aagccgcggc gcaggaaagg caggggcagc
      541 tccacgatcg cctccgagag gcgtttgcgg tttccagggt tcccctgctg ggtaacccag
      601 tccgccatgc gttcaatggg cacttcggtc aggaacatcg agtcctggcg cctgagcaga
      661 agggtctcgc ggtgaccgta ccggacggag gtgatgatcg acgtcagcgg gagcacgccg
      721 gtcggtagga caggccgtcc gaacagggcg ttcatcagcg tgctcttgcc tcggctgaac
      781 tgccctacca ccacaaggtt gaatcggtct tcggcgagct tcgccaggag ggagcggcag
      841 cgctcggcgt cgcgcggatc cgacggctcg atggataggt tcgcctgaag ctcctgaagc
      901 acgtccgcga tcgcgtgccg cgccctggtg taggcctgta gcggattgga atccggcgcc
      961 atcgcctcag cacctctgac gggaagggaa agcgcaattg gacgagatcc ccgttgcggc
     1021 tctggccatc ctcctgattg ctcctctcac ggcgatccct ccccccgtgg gaaatcccga
     1081 gatcaggagt tatcagcttt gcagcggtta gggtgaactc catcgcccac caagatgttt
     1141 accgccaacg gaaaggtctg gtcaagtcgc ccggacggca ggccgacagc ccgcaacgga
     1201 gcaagatcag cgcatccctg gcccgtcggc cctgcgatgc gcgggcggaa ccgagccccg
     1261 ccctccgggg ggtcgcctga cggctctgct tcgggcgggc tcccgcagga gcgcgatcag
     1321 gagcgcggcc gccagcccca gcacgatgct gtttccatag aacggatacg actgccccat
     1381 cgtatcgtag agccaaccgc cggccagcgg acctacggcc gcccccagga agtaggcgaa
     1441 ggtgtacagg ccgtagctcc ttccgcgacg gtcccgtccg gcgatatcag cgacgaacgc
     1501 ccgttcggcg ggtgaggatg cggcgtagcc cagggagtcc acggcccaca ggatggccag
     1561 gggccacacg gcccgaagcc ggggcatcac ggcggaggcc acccccgcgg tgaccagccc
     1621 cgccaccatc aggggctttc gcgccatacg gtccgtcaac ctgccgatgc gggacggcag
     1681 gaacgagctc accagcgccg ccggcaggta ggcgactgcc agctcgaacg cgcccgcctt
     1741 caggcggtct tccaggaaga ccacgaggac cggccagacc atcgcggtgg aagcgcccgt
     1801 tatgaagaca atccccatca gggcgatgag ctgaagggac agccgtcggg gcgcatgata
     1861 ggacgggtcc gactcgtggg agggcaggcg ggacgacccg ccccgcgatg gtcccgtctc
     1921 gccgatccgc caggcgccga gagccaggcc ggctccggcg ccaagcgtgt acactccgaa
     1981 gagccagggc cacaccgctg cccacggcag ccccgtggcc tgcgtcacgg cgatcagtgc
     2041 caccccgggc agcgccccta ccaggctgcc ccgactgctc gcctcgtcga tggcgccgaa
     2101 cccccgggcc cggtcccact cgtgggccac atccgccacg atcgtgtacg cggagagcca
     2161 gaagaatgcc tgtcccagcc cttgcaccac gcgggcgacg gccaggagcg ccacggatcc
     2221 ggccattgcc gtcagagcca tggagaccgc gtaggacgcc aggccggcga ggatgaacgg
     2281 ccgccgtccc cagcggtcca ccatgcggcc caggaacggg cgcaccagca cgggggcgaa
     2341 gctgaccgcg gagaagagcg cgccgacctg aagggcggag gcgccgagca tctttccata
     2401 gatcggaagg gcgaacgcaa ggacggcgaa cgggagcgat gccaggaagg cagtccccca
     2461 gaggggacca aggggactgg cgcgttcctc ccggctcatg cgaacgggta taccggcggc
     2521 ttccgcctcg gtcaagccgc gctcgccggc tctatatccc cctcgtgccg gcactagtat
     2581 ctttgggtat gcgcgctcct gccgcccttc ctcctcgccc gttgcagccg ccgccggagc
     2641 cgccttcgga cctgaagctc ctccgagaac gcttgcggca gatgcccgcc tggacccgga
     2701 cgctcctcct gttcctggcg aacatcgctc tcctagccgc ggggatcctg ctctacgatg
     2761 cgttgaagcc gaagccccag catctgacgc aggcggacat cgattccgcg gtcctgcgaa
     2821 ccctcgatgc cctgccgccc aagccgtcgg atgcgtccgt tgcctacaac gtcattcggc
     2881 cgtccctggt gagcgtggta gcgcggatcg ggaccgggga caaggccaag acgtccctgg
     2941 gaagcggagt cgtcgtggtg gacaccggga tgatcctcac ggcccttcac gtggtggacg
     3001 gcgcatcgga cgtgcaggtc accttcgcgg acggcagcca gtccggcgcg ctggtcgtgg
     3061 tgaggcagcc cgagcatgac ctcgcggtgt tgagcgccat caccgcgccg gatgacctga
     3121 agcccgccac cctggtgagc tcggggacct tgcagcccgg cgacgaggtc atcgccgtgg
     3181 gagacccctt cgggatcgtg aactcggtct cggccggcgt cgtgtcgggg cttcatcgcg
     3241 agtacacgga ccgcaagggc aacgtcaccc tcaaggatct catccagttc gacgcggcgg
     3301 tgaaccccgg caactccggc gggccactcc tcaaccgcaa cggcgaggtg gttgggatcg
     3361 tggattcgct cctcaacccc acggcccagg aggtgttcat cggcatcggg ttcgccgtgc
     3421 cgatcgaaac ggcctcggcg gccctgggat tctcccccat gtaggcggtg gcggacaccg
     3481 gcttgagcgc agcaggaaca aggaaggaaa cgatggagag tagctcggaa cacggcggat
     3541 cgcccgtgtc ggcccagagc ccgaccgccc cctccgcggc ggaaggcggg gcgcagggaa
     3601 cgccggcagg ccgcctgccg gaggaggcgg tcgcggagca ggcgctgctc atggagcaga
     3661 tcctgtacga ggtgaagaag gtcatcgtcg gccaggacca cctgctggag cgcgtcctgg
     3721 tggcgctgct gtcccagggt cacctcctgg tcgagggtgt gcccggcctg gcgaagaccc
     3781 tcacggtgaa gacggtcgcg gacgcggtcc agggaatctt caagcgcatc cagttcactc
     3841 cggacctggt gcccgcggac ctcgtgggga cccggatcta caaccagaag acgggggact
     3901 tcagcacgtc gctcggtccc gtgttcgcca acctcctcct ggcggacgag atcaaccggg
     3961 cgccggccaa ggtgcagagc gccctgctgg aggtcatgca ggaacggcag gtcaccatcg
     4021 gcggccagac gttcaaggtg cccgagccct tcctggtcat ggccacgcag aaccccatcg
     4081 agacggacgg gacctacccc ctgccggagg cgcaggtgga ccggttcatg atgaagaccc
     4141 tcgtggacta ccccagcgag gcggaggagt tcgtgatcgt ggagcgggtg accggaacca
     4201 tggccccggt gaagcccgtg gtgaccacgc agcagatcgt ggagctgcgg ggctggtgcg
     4261 agcgggtctt cgtggacccc atcctggtgc agcacgcggt tcgccttgcc tccgctaccc
     4321 gcgccccgga ccagcacggg gtcccggaca tcgcgaagta catcgtctac ggcgccagcc
     4381 ccagggcttc catcaacctg atcatcggcg ctcgcgccct ggcgttcctg cgcggcagga
     4441 cctacgtggt cccccaggac ctcaccgaca tggtgctgga cgtgatgcgc caccgcatca
     4501 tcccgtccta cgaggcgttg tcggacggcg tgacgaccga tgacatcctg gccaaggtcc
     4561 tggaggccat ccccgcgcct ccgcaggcgc tgaagacgca tgctgcctcc ggcgggtgac
     4621 cccgagcgca tcctgcagcg cctggagtgg acggtcatcc gtcggctcga cgggctgctg
     4681 caggggaact accgtaccct gtttcgaggc ttcggcaccg agctggccga gctgcgggag
     4741 tacgtgctga gcgatgacgt gcgctacatc gactggtacg tgacggcccg tctgcagacc
     4801 ccgtacgttc gccagtacgt ggaggaccgt gagatcaccg cgtggttcct tctggacctg
     4861 agcccgtccc tggatttcgg gacggtcacc acggagaagc gcacgctcct ggcggactgt
     4921 gtcggcgtcc tggcgcgcct cctgacccga cacggcaatc gggtgggcgc cctctttttc
     4981 gcgggttcgc aggagcgttt cattccggcg cagaacggca gaaaccacgt gcttcggatc
     5041 ctggaggacc tcctgcgtca gcctcgactg gcgcgggccc ccatgacgga tctgtccttt
     5101 ccgctcacca cggcgctgcg gatgatccgc cgccgctccc tgatcttcct cgtgtcggat
     5161 ttcatcagcg cgcccggatg ggagaagccg ctatccctcc tgacgcaacg gcacgagctc
     5221 ctggccgtgc gggtcaccga tccgcgggag agcgagctgc cggacatcgg tcccctcgtt
     5281 ctggaggact cggagacggg ggaacagctc ttcgtcgata cccacgaccg ggcgttccgc
     5341 aggcgcttcg ccgatgccgc ggcgtcgcgg gaggcgtcgc tgcaggcgat gctggcgcgc
     5401 cgggggatcg acctcctctc cctgtccacc gagggggacc tggtagccga gctgatgcgg
     5461 ttcgcccaga tccgccggga gcggaagaag cttccgtccc ggcagaacct cccgcgcggc
     5521 gccgcgatgg cggcggccgg ctgaacggtt gggggaaggt atgtcattcc agtggagctc
     5581 ccttctgtgg ttcctgatcc tggtgccgat cctggtcctc ctgtacctct gggcgcagag
     5641 gcgccggcag cgctacgccc tgcgctacgc cagcctctcc ctggtgaagg aggccgtggg
     5701 acggggtccc gggatccgtc ggcacattcc cgcgatcctg
//
LOCUS       DASRZS010000460         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4480319, whole genome shotgun sequence.
ACCESSION   DASRZS010000460 DASRZS010000000
VERSION     DASRZS010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4480319"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFH83_11255"
     CDS             complement(<1..304)
                     /locus_tag="VFH83_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET6486990.1"
                     /translation="MSDDREARGEARCETQLLRHMLLIRRFEEKAAQMYGLRKIGGFC
                     HLYIGQEASAVGAIAAIDLARDYVVGAYRDHGYALSCGLDPRRLMAELYGKASGVSK"
     gene            complement(324..761)
                     /locus_tag="VFH83_11260"
     CDS             complement(324..761)
                     /locus_tag="VFH83_11260"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET6486991.1"
                     /translation="MDSREVMKRVAGIIESHGTGLLATVDEAGDPHVRWLTPTLLPGR
                     QDVLYALTAPRFAKVLQLKTHPKVEWMFQTPTLDEVVNVRGSLHVIENPSLRAEVLEA
                     LGSRLRTFWRLAHDARDLVVLETLIEEATRYLPMDGRKEIVRF"
     gene            complement(793..1500)
                     /gene="plsY"
                     /locus_tag="VFH83_11265"
     CDS             complement(793..1500)
                     /gene="plsY"
                     /locus_tag="VFH83_11265"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET6486992.1"
                     /translation="MIGVALVVVVSYLLGSIPTSIIVGKLFFGKDLREVGSGNAGGTN
                     AFRAFGWKAGVPTVLVDIAKGAAATLLVSRLPAAASIPAPLPMEVLRILAGCAAVIGH
                     IWTVFGGFRGGKGVATAAGMIVSLYPIALLVSAGVFAIALFSTGIASVASLTAAILFP
                     VVLVVLKASGVSTVSPILFWFSIPVALLIVFTHRANIRRLIAGTENRFTRLAIFQRIV
                     RRTRSRRTQDAPPPAST"
     gene            complement(1497..>2263)
                     /locus_tag="VFH83_11270"
     CDS             complement(1497..>2263)
                     /locus_tag="VFH83_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET6486993.1"
                     /translation="RTYLDKLSITPEELTVLQRSSRSHPTTSQPTPADFKARYEFLLR
                     HYRAVLSLSLSGTISGTLDAARAGAKLAGGNVTVVDTRSASVGLGLIARAAAEAVERG
                     AGLEETVALVQGWIERVRIHITVPSLDALVRSGRVSRMKGFAANLLGLKPLIRLDADT
                     EGRPVHGSTVFGVKGGRRKILQLLHREIGDAGPVEFAITHANAPQDAQWFKERLMQEF
                     AVAREPYVVPVTSVLAAHIGEGAAGLAYLLPEPRVP"
BASE COUNT          395 a          743 c          778 g          347 t
ORIGIN      
        1 ccttggagac gcccgaggcc ttgccgtaca gctccgccat gagccggcgg gggtccagtc
       61 cgcaggagag ggcgtagccg tggtcgcggt aggcgcccac cacgtagtcc cgcgccaggt
      121 cgatggccgc gatggccccg accgccgacg cctcctgccc gatatagagg tggcagaacc
      181 cgccaatctt ccgcaggccg tacatctggg cggccttttc ctcaaaacgg cggatgagga
      241 gcatgtgtct cagtagctgc gtttcgcagc gcgcctctcc gcgcgcctca cggtcgtcgg
      301 acatctcgag cgcgctcctt cggtcagaaa cgaacgattt ccttcctgcc atccatgggc
      361 aggtagcggg tagcctcttc gatcagagtc tcgagaacca cgaggtcccg cgcgtcatgg
      421 gccagccgcc agaaggtgcg cagccgggag cccagcgcct ccaggacctc cgcgcgcagg
      481 gacgggttct cgatgacgtg cagggagccc cttacattca ccacctcatc cagggtgggg
      541 gtctggaaca tccattcgac tttcggatgg gtcttgagct ggagcacctt ggcgaagcga
      601 ggcgcggtca acgcgtagag gacgtcctgc cgtcccggca ggagcgtcgg cgtgagccag
      661 cgtacgtgag ggtcccccgc ctcgtccacg gtggcaagca gacccgtccc gtgggattcg
      721 atgatgccgg cgaccctctt catcacttcg cggctatcca tggctccaag atagaataaa
      781 gcccggccgc tttcaagttg aagcgggtgg aggggcgtcc tgcgtgcgtc tggaccttgt
      841 tcgacgcacg atccgctgga agatggcaag ccgcgtgaac cggttctcgg tgccggcgat
      901 cagccggcgg atgttggcgc ggtgggtgaa cacgatcaag agcgcgaccg ggatggagaa
      961 ccagaagagg atcggcgaga cggtggagac gcccgatgct ttcaggacca ccagcacgac
     1021 ggggaagagg attgccgcgg tcagggacgc cacggaggcg atgcccgtgg agaagagcgc
     1081 gatggcgaac acccccgcgc tcaccaggag cgcgatggga tagaggctga cgatcatgcc
     1141 ggccgcggta gccaccccct tgccgcctcg aaagcccccg aacacggtcc agatgtggcc
     1201 gatcacggcc gcgcagccgg ccaggatgcg aagcacctcc atcggaaggg gagcggggat
     1261 ggacgccgct gccggaagcc gcgacaccag aagcgtggcc gcggcgcctt tggcgatatc
     1321 cacgaggacg gtcgggacgc cggccttcca cccgaaggcc cgaaacgcgt tggtgccccc
     1381 cgcgttgccg gagccgacct ctctgaggtc tttgccgaag aagagctttc cgacgatgat
     1441 gctggtggga atcgagccca ggaggtagga gactaccacc accagggcaa cgccgatcat
     1501 gggacccggg gctccggcag aagataggcg agcccggccg cgccttcccc gatgtgcgcg
     1561 gcaaggacag aggtgacggg gaccacgtag ggctcccgcg ctacggcgaa ctcctgcatc
     1621 aatcgctcct tgaaccactg ggcgtcctgg ggagcgttgg cgtgcgttat ggcgaactcc
     1681 accggcccgg cgtcaccgat ctcccgatgc aggagctgca ggatcttgcg gcgaccgcct
     1741 ttcacgccga acacggtgga gccatgcacg ggccgtccct cggtatccgc gtcgagccgg
     1801 atgagcggct tcaagcccag gaggttcgcc gcaaagccct tcatccggct caccctgccg
     1861 ctccgcacca acgcgtccag ggagggcacg gtgatgtgga tgcggactcg ttcgatccag
     1921 ccctggacca gggctacggt ctcctccagg ccggctccgc gctcgacggc ctccgcggcc
     1981 gctcgggcaa tgagccccag gcccaccgac gcggaccggg tgtccacgac cgtcacgttt
     2041 ccgccggcca gcttcgctcc cgcgcgcgcc gcgtccaggg ttccgctgat ggtgccggag
     2101 aggctcaggg agagcacggc ccggtagtgc cggagcagaa actcgtaccg cgccttgaag
     2161 tccgctgggg tcggctgaga ggtggtgggg tgggagcggc tggatcgctg cagcaccgtc
     2221 agctcctccg gagtgatgga gagcttgtcc aggtacgtgc ggc
//
LOCUS       DASRZS010000461         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5637642, whole genome shotgun sequence.
ACCESSION   DASRZS010000461 DASRZS010000000
VERSION     DASRZS010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5637642"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VFH83_11275"
     CDS             complement(<1..359)
                     /locus_tag="VFH83_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486994.1"
                     /translation="MELPDDPPVREMLSWLMTNWGMSKSDLARMFQRTQSTIHHWLAT
                     GQVSHRNLVKIRSSFYFLHETRDPHANERKCYRCERWLPTGEFRAGKAICHGCENSRT
                     LQYYWAHRQEQLTKRKA"
     gene            complement(438..1991)
                     /gene="guaA"
                     /locus_tag="VFH83_11280"
     CDS             complement(438..1991)
                     /gene="guaA"
                     /locus_tag="VFH83_11280"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET6486995.1"
                     /translation="MDKIIILDFGGQSTQLIGRRIRQLGVYSEILPGDTPTSQMNLEG
                     ARGLVLSGSPASVYEPGAPAVDTAIYRLGLPMLGICYGMHRVVQDHGGRVVSLGHREY
                     GRAAVDVRDATQLFGRVPVSSFASWMSHGDSVVELPAGYRVIGESEGGLTAAIASEAD
                     RFWGVQFHPEVSHCEYGTQILEGFVLDIAGARRDWNMGSFLTQQGALIRERVGGHPVV
                     LLISGGVDSSVVAALLLKCLAPSQVHLMYIDTGLMRQGESEEVVENLRRLASVNLHPV
                     DAADRFYRGLAGVTDPEQKRKIIGDLFMKVQEAEIANLGIEDAFLAQGTLYTDLIESG
                     RGVGRKATLIKTHHNVRSPLVEALRERGRIIEPLNTLYKDEVRELGLGLGLPASIVGR
                     HPFPGPGLAVRILGEVTRERCALLRKADELFIRELRARKLYDAIWQAFCVLLPIRSVG
                     VSGDGRRYGNVLALRAVASQDAITADVYPIPMKDLLEISSLITNRVPEIGRVVYDISS
                     KPPATIEWE"
     gene            complement(1991..>2426)
                     /locus_tag="VFH83_11285"
     CDS             complement(1991..>2426)
                     /locus_tag="VFH83_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET6486996.1"
                     /translation="SASTRFVVDYVAGFCLAASRMASVVRSLVVDRRRMAQNLEQAGD
                     LLVSEAVYVLTALGGDPEAHERVRQATLQAEKQRLPLAGILRGDTRLWGLLDRQLRPR
                     GTDAETFFATPGNYRGKASERAHAIADEHERAAQEIREAIGG"
BASE COUNT          424 a          812 c          762 g          428 t
ORIGIN      
        1 ttcgccttgc gcttggtgag ctgttcctgc cggtgagccc agtaatactg gagcgtccgc
       61 gagttttcgc agccgtggca gatcgccttg cccgctcgga actcaccggt tggcagccac
      121 ctctcgcagc ggtagcactt ccgctcgttg gcgtgcggat ctcgggtttc gtgaaggaag
      181 tagaagctgg agcggatctt cacgaggttg cggtgagaga cctgccccgt cgccagccag
      241 tggtggatcg tggattgggt tcgctggaac atgcgcgcca ggtcggactt gctcatgccc
      301 cagttggtca tgagccagga aagcatctcc cgaacgggag gatcgtcggg caactccatg
      361 atcgcctacc tcgtcggcat cattatcggg caggcaccac cattttctga agtgccctgg
      421 ttgggggcac atcggcccta ctcccactcg atcgtggcgg gaggcttgct ggagatatcg
      481 tagacgacgc ggccgatctc gggcacgcgg ttggtgatca gagaggatat ctccaggagg
      541 tctttcatgg gtatcggata gacatccgcc gtgatggcgt cctgcgaggc aacggcgcgc
      601 agcgcaagga cgttgccgta gcgccgtcca tctccggaga cgcccacgga acggatcggg
      661 agcaggacgc agaaggcctg ccaaatggcg tcgtacagct tccgcgcccg gagctcccgg
      721 atgaaaagct catccgcctt ccgcaacagg gcacaccgtt cccgggtgac ctcgcccagg
      781 atccgcacgg ccaagccggg tcccggaaac gggtgtcgtc cgacgatgga cgccggcagc
      841 cccagaccca gacccagctc ccggacctcg tccttgtaca acgtgttgag cggctcgatg
      901 atccgtcccc gctcccgcaa cgcttcgacg agcggcgacc gcacgttgtg gtgtgtcttg
      961 atcagggtgg ctttccgccc cactcccctt cccgactcga tgaggtcggt atacagcgtc
     1021 ccttgtgcca ggaaggcgtc ttcgatcccg aggttggcga tttccgcctc ctggaccttc
     1081 atgaacagat caccgatgat cttccgcttc tgctcgggat cggtcacccc agccaggcct
     1141 cgatagaagc ggtccgcggc atccaccggg tgcagattca cgctggccag gcgccgcagg
     1201 ttctccacga cttcctcgga ctctccctgc cgcatgagcc cggtgtcaat atacatgagg
     1261 tgcacctggc tgggagccag acacttcaga agcagcgccg ctacgacgga agagtcgacg
     1321 ccgccggaga tcagcaggac cacgggatgg ccgccgaccc tctctcggat gagagcccct
     1381 tgctgcgtca ggaaggagcc catgttccag tcccgccgcg cccccgcgat gtcgaggacg
     1441 aatccctcca ggatctgggt cccgtattcg cagtgactca cttccggatg gaactggaca
     1501 ccccagaacc ggtccgcctc gctggcgatt gcggcggtga gtcctccttc ggactccccg
     1561 atgacccggt agccggccgg aagctccaca acggaatctc cgtggctcat ccacgaggcg
     1621 aagctcgaaa ccggaacccg accgaaaagc tgcgtggcgt cccggacatc cactgcggcc
     1681 ctgccatact cccgatgccc gagactgacc acgcggccgc cgtgatcctg gacgactcgg
     1741 tgcatcccgt agcaaatccc cagcatcggc aagccaagcc ggtagatggc cgtatctacc
     1801 gcgggggcgc ccggctcgta gacggacgcg ggagaccccg agagaacgag gccgcgcgcg
     1861 ccctccaggt tcatctgact ggtcggggtg tcgccaggga ggatctcgct gtacacgccg
     1921 agctgccgga tcctccgtcc gatgagctgg gtgctctgtc cgccaaagtc caggatgatg
     1981 atcttgtcca tcagccgccg atcgcctccc gaatctcttg ggccgcgcgc tcgtgctcgt
     2041 ccgcgatggc gtgagcgcgt tccgaggcct tgccgcggta gttgcccgga gtggcaaaga
     2101 acgtctcggc gtccgtccca cgcggccgga gctgccgatc cagcagcccc cagaggcgcg
     2161 tgtctccacg gaggatgccg gccagcggca ggcgttgttt ctccgcctgg agcgtggcct
     2221 ggcggacccg ttcatgggcc tccgggtctc ctccgagcgc ggtcagtacg tatacggcct
     2281 cggaaaccag aaggtcgccc gcctgctcga ggttctgcgc catacgccgc cggtccacga
     2341 ccagggaacg aacaaccgag gccattcggc tggccgcgag acagaagcca gccacgtagt
     2401 caacgacgaa tcgcgtggat gccgag
//
LOCUS       DASRZS010000462         3324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1786278, whole genome shotgun sequence.
ACCESSION   DASRZS010000462 DASRZS010000000
VERSION     DASRZS010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786278"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFH83_11290"
     CDS             complement(<1..342)
                     /locus_tag="VFH83_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486997.1"
                     /translation="MSGGGPSPRPLGKHEEAVDRFYASGIRRDWSFIETLEDRFEGYL
                     NFGYWDAGARDYVRAAKDLIALLVAESGIREPRRILNVCCGYGSETFQYFRELRPRSI
                     VGVDITEVKIHY"
     gene            complement(339..1433)
                     /locus_tag="VFH83_11295"
     CDS             complement(339..1433)
                     /locus_tag="VFH83_11295"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET6486998.1"
                     /translation="MRNLLDDHQLTRKFTREEVDLLTSLLGTRRYGAGECIMRENEVY
                     HSIFFIASGGVEVLKREPKTGKEFKLVDFGEGAFFGEMSFVTDNEPASATIRATGDVE
                     VAVLPKESIASLPLYHKLVLNISSNQMARMRETNNRYVESLSAQVERLKVSKEFGQLF
                     IITVILFGLQSVVPDTSEGATPLLQIGFSWLRLLVYLVPFVPLLVLQRRPLATYGVTG
                     VGWKRSLLESLVLLVVAAPLFTGFKYLTVPGQALFPFTYFAGYPLGLTILYFAIYPIH
                     SFLQEFMLRGVMQGALQRFMIDSKPVVPITVTSLIFGMGHLYKSPMVAILTFAISFLL
                     GAIYHRHKSLVGVTVLHYFLGSLAQALGYL"
     gene            complement(1433..2461)
                     /locus_tag="VFH83_11300"
     CDS             complement(1433..2461)
                     /locus_tag="VFH83_11300"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6486999.1"
                     /translation="MKGRARSASDTLLTPALRRYLAAPILRLVPSRVSANAVTLAAHA
                     CVYAVPFVAATVGQTRPALAASVIVLLILLFAIGDELDGMQAQRTRTTSSLGEFLDHY
                     LDSFCPGALLYTAYALYRLPATDLLLLTIGFTFLTGATLYHEHLRTGTMVFEAISTTE
                     SLAFAAVVLGLGAIPEVQRLARLEVLPGYGVLTLFLLAVSVAVLVPTAVRSFVRTRAI
                     PIPWLLLAFGMAGVMAGLLIASAGLSLGAAVLLLYGCSNAGALILRRLRPGRMPVPDP
                     VAPVALLVSALAVPLYAPVVQAAVLVYLGARCAWVAGAAALRLPRPGRPVVSGTAAAA
                     ALPDQKEA"
     gene            complement(2458..>3324)
                     /locus_tag="VFH83_11305"
     CDS             complement(2458..>3324)
                     /locus_tag="VFH83_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6487000.1"
                     /translation="PPHGRLLVLANGAYGERMVEIAAAYRIDATVVRSDPAIPVDPAA
                     AGDALARDPGITTVAMVHHETTTGVLNPVGAVGRMVRAHGAALVVDAISSFGGIPFSM
                     EDCGVDFLLSTANKCLQGMAGAAFVISRKDALDAIAPLPRRSYYLSLADLWEHFERTS
                     QTRFTPPVQTLYALRQALDELFQEGVEARYARYCESWRVLRSGLERLGLSILTRPEDE
                     SRLLLTVRYPSPAFDFDALHDVLYAEGYTIYPGKIGGAGTFRLSVIGAIDADDIRAFL
                     DALRHALGAAGR"
BASE COUNT          630 a         1073 c         1139 g          482 t
ORIGIN      
        1 gtagtggatt ttcacctcgg tgatgtccac cccgacgatg ctccgcgggc gaagctcccg
       61 gaagtactgg aacgtctcgc tcccgtagcc gcagcacacg ttcaggatcc gccggggctc
      121 tcggatcccg ctctcggcga cgaggagggc gatgagatcc ttcgcggccc gtacataatc
      181 gcgcgccccg gcatcccagt acccgaagtt cagatatcct tcgaagcgat cttccagcgt
      241 ctcgataaag gaccagtcgc gtcggattcc ggacgcatag aaccggtcga ccgcctcttc
      301 gtgcttgccc agcggacgcg gtgacggccc cccgccgctc aaaggtaccc cagcgcctgc
      361 gccagcgatc ccaggaagta gtgcagcacc gtaaccccga ccaggctctt gtggcggtgg
      421 tagatggctc ccagcaggaa gctgatggcg aaggtgagga tcgccaccat cggcgacttg
      481 tagaggtgtc ccatcccgaa gatgagggag gtcacggtga tggggaccac cggcttggag
      541 tcgatcatga agcgctgaag ggcgccctgc atgacccctc tcagcatgaa ctcctgcaga
      601 aaggagtgga tcgggtagat cgcgaagtac aggatcgtga gacccagcgg gtagccggcg
      661 aaataggtga acgggaacag cgcctgccca ggaaccgtga ggtacttgaa ccccgtgaag
      721 agaggggcgg ccacgacgag gaggaccagg ctctcaagga ggctccgctt ccacccaacc
      781 ccggtcaccc cgtaggtagc cagggggcgc ctctgcagga ccagcagggg cacgaacggg
      841 accaggtaga caagcaggcg cagccagctg aacccgatct gcaggagcgg cgtcgcgcct
      901 tccgaggtat ccggcaccac ggactggagt ccgaagagga tgaccgtgat gatgaagagc
      961 tggccgaact ccttgctgac cttgagccgc tccacctgtg ccgacaggct ctccacgtat
     1021 cggttgttgg tctcccgcat ccgcgccatc tggttgctgg agatgttgag gaccagcttg
     1081 tgatagagcg gaaggctcgc gatgctctcc ttcggcagga cggccacctc cacgtctccg
     1141 gtggcgcgga tcgtggcgga ggcgggctcg ttgtccgtga caaaggacat ctcgccgaag
     1201 aaagcgccct ctccgaaatc gacgagcttg aactcttttc ccgtcttcgg ctcgcgcttc
     1261 aggacttcca ccccgccgct ggcgatgaag aagatggaat ggtagacctc gttttcccgc
     1321 atgatgcatt cccccgcgcc gtagcgacgg gtgcccagca ggctcgtaag gagatctacc
     1381 tcctcccgcg tgaacttccg ggtgagctgg tggtcatcca gcaggttgcg catcaagcct
     1441 ctttctggtc ggggagagcg gccgctgccg cggtgccgga caccacgggc cgcccaggcc
     1501 gcggcaggcg gagtgccgcc gcccctgcga cccaggcgca ccgggcgccc aggtacacca
     1561 ggacggccgc ctggacgacc ggggcataga ggggaaccgc cagcgctgaa acgagcagcg
     1621 caaccggcgc gacagggtcc gggaccggca tccttcctgg cctgagccgg cgcaggatca
     1681 gggcccccgc gttcgagcag ccatagagca gaagcacggc ggcgcccagg gagagtcccg
     1741 ccgaggcgat caggagcccg gccatcaccc cggccatccc gaatgcaagg agcagccacg
     1801 ggatcgggat tgcccgtgtt cggacaaagg aacggacggc ggtgggtacg agcaccgcca
     1861 cgctcaccgc aagcaggaac agcgtgagga cgccgtatcc gggaaggacc tccagacgag
     1921 ccagtcgctg gacctcggga atggccccaa ggcccaggac gaccgccgca aaggcgagac
     1981 tctccgtggt ggagatcgcc tcgaagacca tggtcccggt ccgcaggtgc tcgtgataga
     2041 gcgtcgcgcc cgtcagaaac gtgaacccta tcgtcaggag caggaggtcc gtggcgggca
     2101 gccggtacag cgcgtaggca gtgtacagaa gcgcccccgg acagaacgaa tccaggtagt
     2161 ggtccaggaa ctcgcccagg gaggacgtgg tgcgggtccg ctgcgcctgc atgccgtcca
     2221 gctcgtctcc gatcgcgaag aggaggatca gcaggacgat cacggaagcc gccagcgcgg
     2281 gccgtgtctg cccaacggtg gcggccacga acgggacggc gtacacgcat gcgtgggccg
     2341 ccagcgtgac ggcgttggcc gagacgcggc tcggcaccag gcgcaggatg ggtgccgcca
     2401 ggtagcggcg gagcgcgggg gtgagaagcg tatcactggc ggagcgcgcc cgccccttca
     2461 tcggccggcc gcgccgagcg cgtggcgcag cgcgtccagg aacgcgcgga tgtcgtcggc
     2521 gtcgatcgcc ccaatgacgg acaggcggaa cgtaccggcc ccgccgatct tcccggggta
     2581 gatcgtatag ccttcggcgt agaggacatc gtggagagcg tcgaagtcga aagccggcga
     2641 agggtagcgg accgtgagca ggagccggga ctcgtcctcg ggccgcgtca ggatgctcag
     2701 gcccagccgc tccaggccgg accgcaggac acgccagctc tcgcagtagc gcgcgtatcg
     2761 agcctccact ccctcctgga agagctcgtc cagcgcctgg cgcagcgcgt acagcgtctg
     2821 gaccggcggc gtgaagcgcg tctggctggt ccgctcgaag tgctcccaga ggtcggcaag
     2881 actgaggtag taggagcggc ggggcagggg tgctatcgcg tccagcgcat ccttacggct
     2941 gatcacgaac gcggctcccg ccatgccctg cagacacttg ttcgccgtgg aaaggaggaa
     3001 gtcgactcca cagtcttcca ttgaaaaggg aatcccaccg aagctggaga ttgcatcgac
     3061 gaccaacgcg gcgccgtgag cccggaccat gcgtcccacc gcgccaaccg ggttgaggac
     3121 acccgtggtc gtctcgtggt gcaccattgc cacggtggtg atgccggggt cccgcgcaag
     3181 cgcgtcaccg gctgcggccg gatccacggg gatggcaggg tcggaccgca cgaccgtggc
     3241 gtcgatccgg taggcggcgg cgatctcgac catccgctcc ccgtaggcgc cgttggcaag
     3301 aaccagcagt ctcccgtggg gagg
//
LOCUS       DASRZS010000463         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1786502, whole genome shotgun sequence.
ACCESSION   DASRZS010000463 DASRZS010000000
VERSION     DASRZS010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786502"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="VFH83_11310"
     CDS             <1..1516
                     /locus_tag="VFH83_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487001.1"
                     /translation="SGKPSAEAARFLNETTGPDKPRVYRNAVLLLAPSRDGIELALAR
                     VRDYLGWEIVREEIKKQQKDGNVDPARAQTLQINIDRAKGRMPEAIKQAYCIVVTVSE
                     KDDVQAFKITVTEEPHFTIIKGDKRSRVQDTAVEAAALLPDGPYNLWRTGEPSRRVKD
                     LAGAFAQLPHLPKMLKAGAILDTLADGCERGTFVLRLVRPDGTSRTWWMSRPDEYALS
                     DSALELVLPQGAELGELGPALLVPGKLPGLWPAEEITVHAVADYFNGSTVIQVERQGY
                     QEPMPIPKVGQAAVEKAISTAVEGGNLWLLSAPASILAEPIPPGVLNPSARLSAPPAI
                     IAPAEILPENLPSPWKGGIASGLSIATALSVKVGKTLPWKTVRDSINAALQARFLEVA
                     EGSQAWPCDLPSAQFAKFKVASGADRVDTGWRGVADVAPGMIVVAAVELQPAQVQDLG
                     DIIPKLLEVRAKVGMPIRFHVRIEVGDGQNVPAKDIIRDVNALLQHIDKNLTLR"
     gene            complement(1550..>2181)
                     /locus_tag="VFH83_11315"
     CDS             complement(1550..>2181)
                     /locus_tag="VFH83_11315"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HET6487002.1"
                     /translation="DIWYRAQGDRFVHISLIEPQAGLIRGISIFQLSPSFDLIRRVDA
                     REALWGQEGWTLRDGYQLDLKTNPIRITPFRELRVELREHPEEFARVARSPEEMSYAQ
                     LREYIDKLVKSGVSATRYVIDLHAKVAIASVSLIMAALGVSFGLRTGRSGVALWVGVC
                     IPLGFLYYMLLSVGISLGRGGVIPPLLAPWIPNLLFGTASVVSLWRLRG"
BASE COUNT          436 a          660 c          731 g          354 t
ORIGIN      
        1 gtccggcaag ccgagcgccg aggcggcgcg attcctgaac gagaccacgg ggccggacaa
       61 gccccgcgtg taccgaaacg cggtccttct cctggccccg tctcgtgacg ggattgagct
      121 ggcattggcg agggtgcgcg actacctggg ctgggagatc gtccgggagg agattaaaaa
      181 acagcagaaa gacggaaacg tcgatccagc cagagcccag acgctccaga tcaacattga
      241 cagggccaaa gggcggatgc ccgaagcgat caagcaagcg tattgcatcg tggtgaccgt
      301 ctcggagaag gacgacgtgc aggccttcaa gatcaccgta acggaggagc cgcactttac
      361 tatcatcaaa ggcgacaagc ggtcgcgcgt tcaggatacc gccgttgagg cggcagcgct
      421 tctcccggac ggtccatata acctgtggcg gaccggagag ccgtctcgcc gcgtcaagga
      481 cctggcggga gcatttgcgc agcttccgca cctgccaaag atgctcaagg ccggggctat
      541 tctggatacg ctggccgatg ggtgcgagcg gggtaccttt gtcctgcggc ttgtccgtcc
      601 ggatggaaca tcccggacct ggtggatgtc ccgaccggac gagtatgcct tgagcgattc
      661 cgcactggag cttgttctcc ctcagggggc ggagcttgga gagctgggac cggccctcct
      721 ggttcctggg aagctaccag ggctgtggcc ggctgaagag attactgtgc acgcggtggc
      781 cgattacttc aatggctcaa cggtgattca ggtcgagcgc cagggatacc aggagccgat
      841 gcccataccg aaagtggggc aagccgcagt cgaaaaggcg atcagcacgg cggtcgaagg
      901 cggaaacctc tggttgcttt ccgccccggc cagcattctg gctgagccga taccgccggg
      961 cgtcttgaac ccgagtgccc ggctgagcgc cccgccggcc atcattgccc cggccgagat
     1021 cctgccggag aatctcccga gcccctggaa gggtggaatc gcctcgggcc tctctatcgc
     1081 gacggccctg tccgtgaagg tggggaagac gttaccatgg aagaccgttc gggacagcat
     1141 caacgccgcc ctgcaggcac ggttcctcga agtagcggaa gggtcgcagg cctggccgtg
     1201 cgaccttccg tcggctcagt tcgcgaaatt caaggtggcg agcggggctg accgcgtgga
     1261 caccggctgg cgaggagtgg cggacgtcgc tcccggaatg atcgtggtgg cggccgttga
     1321 gttgcagccg gcgcaggtgc aggatctggg ggacatcatc ccgaagctac tggaagtccg
     1381 ggccaaggtg ggtatgccga ttcggttcca cgtacggatc gaggttggtg acggacagaa
     1441 tgttccagcc aaggacatca tccgggacgt caacgcgctg ctccagcata tcgacaagaa
     1501 cctcaccttg cgttagcgaa gcggaggggg gacacagtcg cgatgggact cacccccgta
     1561 ggcgccagag ggagacgacg ctggcggtgc cgaagagcag gtttgggatc cagggggcga
     1621 ggagcggggg gataacgccg ccccgcccga gggagatccc cactgagagg agcatgtagt
     1681 acaggaaccc caacggaatg cagactccga cccagagcgc gacgccggaa cgcccggtcc
     1741 gcaggccgaa actgacacca agggccgcca tgatgaggct caccgaggca atggccacct
     1801 tggcgtgtag gtctatcaca tatcgagtag cgctcacgcc gctcttgacc agcttgtcaa
     1861 tatactcccg gagctgagca tagctcatct cttccgggga gcgtgcgacc cgggcgaact
     1921 cctcggggtg ctcccgcagc tccacgcgca actcccggaa gggcgtgatg cggatcgggt
     1981 tcgttttcaa gtccaactgg tagccatcac ggagcgtcca cccctcctgg ccccacaagg
     2041 cctcccgcgc gtccactcgc cggatcagat cgaagctcgg cgacagctgg aagatgctga
     2101 taccccggat caagccggcc tgcggctcga tgagggagat gtgcacgaac cgatccccct
     2161 gggcgcggta ccagatgtcg c
//
LOCUS       DASRZS010000464         7964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1788425, whole genome shotgun sequence.
ACCESSION   DASRZS010000464 DASRZS010000000
VERSION     DASRZS010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788425"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..765)
                     /locus_tag="VFH83_11320"
     CDS             complement(13..765)
                     /locus_tag="VFH83_11320"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HET6487003.1"
                     /translation="MLIFGIDCAAQEKNVGLAAGRFDGKKVRFTDLRPDSSGGSVCTR
                     ILDNLSGEPTALLALDAPLGWPKALGQELRRHSAGRPIATQPNDLFRRLTDHATRASI
                     SKVPMDVGADRIARAAHAALRLIGDLEAGLGCRIPLAWAPPVGPGVWAIEVYPGGFLK
                     ANGVTASGYKEPDAQGMRRTMLEALRAFYPLEEPEGRDCTVDQHLFDAALCVLSATDF
                     LTGRSVPPREAQRARAVKEGWIWTRGPRLPML"
     gene            complement(786..1880)
                     /locus_tag="VFH83_11325"
     CDS             complement(786..1880)
                     /locus_tag="VFH83_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET6487004.1"
                     /translation="MAKGFALEIDPWVYRAKFQEGGLWAEEYIEKPHLTPGEERALPA
                     AERDALFLKRNNFADLPLVNYTTQYGFGCFEGLKAFPQPDGSLRLFRPDQNGLRMEAS
                     MKGLAMPPYPSGLFVGAVRGVVAKNKAMGFAPCYDAAWVKDDFTTADAVYVRPFSYAE
                     PGIGVGISKNPWVIVVTTPVGSYFKAGNSRATTTKRVRANPGGTGWIKSDSNYVNSAL
                     AKREAEQGGFMEAIFLDAKTQSYFEEGSSCNIFFVMKNGTLVTPSLEDTILPGITRRS
                     LLELGKEKGLKPEERRVSVQEAFSDARECFVSGTAAGITFIESITHDGREAVFANRVM
                     GEVTRDLLKTLKGIQYGALRDTHGWMVAAG"
     gene            2120..2413
                     /locus_tag="VFH83_11330"
     CDS             2120..2413
                     /locus_tag="VFH83_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487005.1"
                     /translation="MEHTRDHIIFFMAQKMYFLERNGNQELFNRLVEHFEQINLDGLL
                     EYLTRYYALDVERELDDFYLEQGELVFVSRRKDQRFKHVFRDGHLRLVGIEMA"
     gene            complement(2423..3382)
                     /locus_tag="VFH83_11335"
     CDS             complement(2423..3382)
                     /locus_tag="VFH83_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487006.1"
                     /translation="MVELEKRISGDWIEIKSKANNLPGLLEFLAVPALTMRSASGKLR
                     HCVEQGIVEFKRTLLAPVLVALETSRYKSLDGAEMGEHEGSLSLMLLAILVQRLLATQ
                     VLPLSKQQKRKAVVDVETVGLNAILADINARVKANPAIRANLAVKNILMQVQLYTREN
                     QKMRELLPTIKPDMRTAFLANFTRTFGNIVGSIRRQYATLLEEQVESLQSSQDTFSLA
                     NLPMKDLAPLLATQAREISRMRSTFGHARDEKYRTREILVRLYESRQGMLKMIEDESR
                     MYRRVCQKGLQPDLDACALWIANAYRDEIAGILDRQGKRDSAT"
     gene            3672..4127
                     /locus_tag="VFH83_11340"
     CDS             3672..4127
                     /locus_tag="VFH83_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET6487007.1"
                     /translation="MNNLNSILIEGNLVRDPELSYTTKGTALCKFSVASNRFYKQDQE
                     TQKEVSFFDVTTWNRLAEVCGEYLKKGRGVRVVGRLKQDRWTDPEGKPHSRIFIIAEH
                     VEFRPQLKNKDGSEGTPAEAEGVKGEEESVSAEQEGQGELEDLKEAAGF"
     gene            complement(4152..4577)
                     /locus_tag="VFH83_11345"
     CDS             complement(4152..4577)
                     /locus_tag="VFH83_11345"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HET6487008.1"
                     /translation="MDRNRWFGTWRLESMELHSLGGVTTYPYGRNPTGYIMYLEPDRM
                     AVTLGSGDRPPFAAEDMLGGTDQEKSAAFSSFLSYCGTFQVGEHSVVHHIDVSLFPNW
                     VGTRQERLYEFEGDALRLSSRPFRINGEQQTVHLVWRRV"
     gene            4708..5544
                     /locus_tag="VFH83_11350"
     CDS             4708..5544
                     /locus_tag="VFH83_11350"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6487009.1"
                     /translation="MKRGIVYATVGTRDLALDLYLPNDPFRAVPIIVFIHGGGWSALD
                     RGWCPFAMRMLAEEYAVASIDYRLLQEGGAFPANLHDCKAAVRWLKRHAADYGLDGEH
                     VAVWGDSAGAHLAAMVGLTGDVPELEGTVGVTGSSLVQACCCYHGAMDLLAWAGPDPD
                     ASPLRELFEKLIGARPLSRNLDKLRAASPVTWAGKRAAPSLLLHGDIDTEVPLSQTVL
                     LYEALWKAGADVRLHVVHGGDHLHYKRSPPDLAWQAPEVRALEDAFFYRTLKGHERHP
                     EV"
     gene            complement(5569..6057)
                     /locus_tag="VFH83_11355"
     CDS             complement(5569..6057)
                     /locus_tag="VFH83_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487010.1"
                     /translation="MKRSVLVVTALIVCGSLFAQGNAKTGDPSLTRQLDRMGVDYTIT
                     DSGNYSIDYTLEGGRSQTTYIMGETQTVDNLQVREIWSRAGTFDSLPSADVMQSLLED
                     SGTREIGFWSIEKAKDGGYILYFSVKVPVYLKDDDLASLLQYTADTADQKEQELFNTD
                     QE"
     gene            6140..6925
                     /locus_tag="VFH83_11360"
     CDS             6140..6925
                     /locus_tag="VFH83_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487011.1"
                     /translation="MTSRKLTPGEISSSVEKIRKKYDQYVTKFYKSRFLRSAFEDRYL
                     KALRAGVDISSFLLAEISAIEELLQREEARVAAAPSKPAPPSAPTPGFADRVLEENRK
                     RITGYPEVSFHPDASEEVRRLLGALVALHGDTWQRLGHALRKTMYSMSSSEMIRLDSD
                     LRSLAAGDLDTPPQALLQLVVQLKRFPRNYAAVEREERDYMLAAAFFLNDLHGALDQV
                     KRLYTEMTEEDRRFLDETMTHVWEIVSDFRLKELKRRDHSETR"
     gene            6985..7311
                     /gene="trxA"
                     /locus_tag="VFH83_11365"
     CDS             6985..7311
                     /gene="trxA"
                     /locus_tag="VFH83_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET6487012.1"
                     /translation="MSEEVAVTKSNFDAEVIKSEVPVVADFWAEWCGPCRMIAPVLKQ
                     LAQEYKDRIKIAKIDVDKEGELAEKYNIVSIPTILVFNKGEVVKQQIGAVPRPILEKM
                     IKDVVA"
     gene            complement(7292..>7964)
                     /locus_tag="VFH83_11370"
     CDS             complement(7292..>7964)
                     /locus_tag="VFH83_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487013.1"
                     /translation="SGNHPDLVELKALTDGDMGGVVFCVGTQGNYQQRLVFPLFAIHK
                     GAFILVHCRPTGDPVEVNETDNLSASGGQDASQTAVDVWLAGGTGLPGNNGALTLYDR
                     PGGAILDAILYSNRTATSDELYRGFGSDQMLGWAEELAGQGAWTAAAQKVSPEDAVSP
                     EGSTATRSLGRSSASTDTNTAADWHIVPTRASSFGSENSDEVYVPMTAENAAGPKGSP
                     TPPRP"
BASE COUNT         1392 a         2556 c         2589 g         1427 t
ORIGIN      
        1 ctcacctgta cactatagca tcggcagacg gggaccccgg gtccagatcc acccttcctt
       61 gacggcgcgc gcccgctggg cctccctggg aggcaccgaa cgacccgtaa ggaagtccgt
      121 ggcggagaga acgcagaggg cggcgtcgaa aaggtgctgg tccacggtgc agtcccgccc
      181 ttcaggctcc tccagaggat agaacgctcg cagcgcttcc agcatcgtcc gccgcatgcc
      241 ctgcgcgtcc ggctccttgt agccggaggc cgtcaccccg ttcgccttca ggaagccgcc
      301 ggggtacacc tcgatggccc agacaccggg gccgacgggc ggcgcccagg ccagaggaat
      361 cctgcaaccg agcccggcct cgaggtctcc gatgagcctc agcgccgcgt gcgcggcgcg
      421 ggcaatccgg tccgcgccca cgtccatggg gactttcgag atcgaggccc gggtggcatg
      481 gtccgtcagc cttcgaaaca agtcgttcgg ttgggtggcg atgggtcttc ccgcggagtg
      541 gcgccgaagc tcctgcccca gggccttggg ccaccccagg ggagcatcga gggcaagcag
      601 ggcggtgggc tctcctgaca ggttgtcgag gatgcgggtg cagacgcttc cccccgatga
      661 gtccggccga aggtccgtga agcgcacctt cttcccgtcg aaccggccgg cggcaaggcc
      721 gacgttcttc tcctgagccg cgcagtcgat gccgaagatc agcatcctgg aggaaaaccc
      781 gggaatcagc cggctgccac catccacccg tgggtgtccc ggagggcccc gtactggatc
      841 cctttcaggg tcttcagcag gtcccgggtg acctcgccca tgacgcggtt ggcgaacacc
      901 gcctcccgcc catcgtgggt gatggattcg atgaacgtga tccccgcggc cgtgccggaa
      961 acgaagcatt cccgggcgtc ggagaatgcc tcctgcacgg acacgcgacg ttcctccggc
     1021 ttcaggccct tctccttgcc gagctcgagg aggctcctgc gggtgatccc cggtaggatc
     1081 gtgtcttcca ggctcggcgt caccagtgtc ccgttcttca tcacgaagaa gatgttgcag
     1141 gaggagcctt cctcgaaata ggactgggtc ttggcgtcca ggaagatggc ctccatgaag
     1201 cctccctgct ccgcctcgcg cttggccagc gcgctgttca cgtagttgga gtccgacttg
     1261 atccagccgg ttccgccggg gttggcccgc acgcgcttgg tggtggtcgc ccggctgttg
     1321 cccgccttga agtaggagcc caccggcgtc gtgaccacga tcacccaggg attcttgctg
     1381 atcccgaccc cgatccccgg ctcggcgtag ctgaacggcc gcacgtagac cgcgtccgcc
     1441 gtggtgaaat cgtccttgac ccacgccgcg tcatagcagg gcgcaaaccc catggccttg
     1501 ttcttcgcta ccacgcctcg cacggcgccc acgaacagcc ccgaggggta cggcggcatg
     1561 gcaagccctt tcatggaagc ctccatgcgc agcccattct gatcgggacg gaaaagcctc
     1621 agggagccgt cgggttgcgg gaaagccttc aaaccctcga agcatccgaa gccgtactgc
     1681 gtcgtgtagt ttaccagggg aaggtcggcg aagttgttgc gcttcagaaa aagcgcatcc
     1741 ctctccgcgg cgggcagggc ccgctcctcc cccggggtca gatgcggttt ctcgatgtat
     1801 tcctcggccc acagcccgcc ctcttggaac ttggcgcggt agacccatgg atcgatctcg
     1861 agcgcgaatc ccttcgccat gaccagcctc ctgcgtcagt atataccaga tgcggcggcg
     1921 aggtccaacc ggcgacgcgc ccttgcggcc acgcgcccct gcggcccgcg gcccctccct
     1981 cccccgcgtc cctcgctccc ccgcggccca cgctcccccc gcgtccctcg ctccccggcg
     2041 tccctcgctc cccctctccc agccgcggct ttttcccgct ggagattatc ggggtaggat
     2101 gtcgatcata gagaggacca tggaacacac acgggaccac atcatcttct tcatggccca
     2161 gaagatgtac ttcctggagc gcaacgggaa ccaggagctc ttcaaccggc tggtcgagca
     2221 cttcgagcag atcaacctgg acgggctcct ggagtacctc acgcgctact acgccctgga
     2281 tgtggaacga gagctggatg acttctacct ggagcagggg gagctggtgt tcgtgtcccg
     2341 ccgcaaggac cagaggttca agcacgtgtt tcgggacggg cacctgcggt tggtggggat
     2401 tgagatggcc tgaggcgcgg cctcaggtcg cgctgtcccg cttgccctgt cggtccagga
     2461 ttccggcgat ctcgtctcga taggcgttgg cgatccagag cgcgcacgcg tccaggtccg
     2521 gctgcagacc cttctggcag accctgcggt acatccggga ctcgtcctcg atcatcttga
     2581 gcatcccctg acgactctcg tagaggcgca ccaggatctc acgggtgcga tacttctcgt
     2641 cccgggcgtg gccgaaggtc gagcgcatgc gggagatctc ccgtgcctgg gtggccagga
     2701 ggggcgccag gtccttcatg ggcaggttgg ccagggagaa ggtgtcctgg ctgctttgaa
     2761 ggctttccac ctgttcctcc aggagcgtcg catactggcg acggatgctt cccacgatgt
     2821 tgccgaaggt gcgggtgaag ttcgcgagaa acgccgttcg catgtccggc ttgatcgtgg
     2881 ggagaagctc ccgcatcttc tggttctccc gcgtatagag ctggacctgc atgaggatgt
     2941 tcttcaccgc caggtttgcg cggatggcgg gattggcctt cacgcgagcg ttgatgtccg
     3001 ccaggatcgc gttcagtccg accgtttcga cgtccacgac cgctttacgc ttctgctgct
     3061 tggacagcgg aaggacctgg gtagccagga gccgctggac cagaatggcc agcagcatca
     3121 ggctgaggga gccctcatgc tctcccatct cggcgccgtc cagggacttg tagcgggacg
     3181 tctccagggc caccagcacc ggagcaagca gggtgcgctt gaactcgacg attccctgct
     3241 ccacacagtg gcgcagcttc ccggaagccg agcgcattgt cagggcggga acggcgagga
     3301 actccaggag gccgggtagg ttgtttgcct tgctcttgat ctctatccag tctccactga
     3361 ttctcttttc tagctccacc atgccagacc gattttatgg attttccatt cgcagatcaa
     3421 tcgacaaaag cgcggcttgc gatttcgtcg atgccatgag gatggcggag ggcaccatga
     3481 ggaacgccgc atgcgcccct gagcctctcg atttctacga aaacgcacgg tgcgattccg
     3541 cagaaatcac gagggccggc ggtcccatcg cgcgtgccgg aaaccggcaa cgggttcaat
     3601 cagcgcgccc gccacgccgg ttcaatggcc aaaggccgcc cgcaagagcg ggcgagagga
     3661 ggaatgtcgt gatgaacaat ctcaactcga ttctgatcga agggaacctg gtaagggacc
     3721 cggagctttc ctacaccacc aagggaaccg cgctgtgcaa gttctccgtg gcgtcgaacc
     3781 ggttctacaa gcaggaccag gagacgcaga aggaggtttc cttctttgac gtcaccacct
     3841 ggaaccggct cgcggaggtc tgcggggaat acctcaagaa gggtcgcggc gtgcgcgtcg
     3901 tgggcaggct gaagcaggac cgctggacgg acccggaggg caagccccac agccggatct
     3961 tcatcatcgc ggagcacgtg gagttcaggc cccagctcaa gaacaaggac ggctcggagg
     4021 gaacgccggc agaggccgaa ggggtgaagg gggaggaaga gtccgtgagc gcggaacagg
     4081 agggacaggg agagctggag gacctgaagg aggctgcagg attctgacgg cggttcgagg
     4141 tcccggggca gctagaccct tctccagacc aggtgaacgg tctgctgctc cccgttgatc
     4201 cggaagggcc gggagctcag gcggagggcg tcgccctcga actcgtagag ccgctcctgg
     4261 cgggtcccca cccagttggg gaacagggat acgtcgatgt ggtggaccac ggaatgctcc
     4321 cctacctgga acgtgccgca ataggaaaga aagctgctga aagcggcgct cttctcctgg
     4381 tccgttccgc ccagcatgtc ctcggcagcg aagggcggcc tatcgccgct ccccagggtt
     4441 acggccatcc gatccggttc caggtacatg atgtaacctg ttggattccg tccgtagggg
     4501 taggtcgtga cgccgcccag gctgtggagc tccatcgact ccagcctcca ggtgccgaac
     4561 caccggtttc tgtccatctc ctctatcgta agaagcggcg cgcccggagt ccaccgccgc
     4621 gcccttcgcg cccatgcgag ttgtggaacc cagggcgggg cggtatgctg ccgggccgga
     4681 ggcgctaccg tgaactcagg acataccgtg aagcgtggga tcgtctatgc aaccgtcggc
     4741 acccgggatc tcgcgctgga tctatacctg cccaacgacc cgttccgcgc tgtacccatc
     4801 attgtgttca tccacggcgg cggttggtcg gcgctggacc ggggctggtg cccgtttgcc
     4861 atgcgcatgc ttgcggagga gtacgcggtt gccagcatcg actatcgcct gctgcaggaa
     4921 gggggggcct ttccggccaa cctgcatgac tgcaaggcgg cggtgcgttg gctgaagagg
     4981 cacgcggcgg actatgggct ggacggagag cacgtcgccg tttggggaga ctcggccggc
     5041 gcgcacctgg ctgcaatggt gggcctgacc ggcgacgtcc cggagctgga aggtaccgtc
     5101 ggcgtgacgg ggtccagcct ggtgcaggcg tgctgctgct accacggcgc catggacctc
     5161 ctggcgtggg ccgggccgga cccggatgca agcccgttgc gggagctctt cgagaagctc
     5221 atcggcgccc ggcccctgtc gcggaatctg gacaagctgc gcgctgccag cccggtgacc
     5281 tgggccggca agcgcgcggc gccatccctg cttctgcacg gggacatcga tacggaggtg
     5341 ccgctgagtc agacggtgct tctctacgag gcgctctgga aagccggcgc ggatgtccga
     5401 ctgcacgtcg tgcatggagg agatcacctg cactacaagc gcagcccacc ggatctcgcc
     5461 tggcaggctc cggaggttcg ggccctggag gacgcgttct tctatcgcac cctcaaggga
     5521 cacgagcgtc acccggaggt ctgacgcacg cagacggacg cgggccgctc actcctggtc
     5581 agtgttgaag agctcctgct ccttctggtc agcggtgtcc gcggtgtact gcaggaggga
     5641 ggccaggtcg tcgtccttca gatagacggg aaccttcacg gagaagtaga ggatgtatcc
     5701 gccgtccttg gctttctcga tgctccagaa gccgatctcc cgggtcccgc tatcctccag
     5761 gaggctctgc atcacgtcgg cggacggaag gctgtcgaag gtgccggcgc gggaccagat
     5821 ctcccgcacc tgcaggttgt ccacggtctg cgtctccccc atgatgtacg tggtctggct
     5881 tcggccgcct tccagggtgt aatcgatgga gtagttgccg gagtccgtga tcgtgtagtc
     5941 cacacccatg cggtcgagct ggcgcgtcag gcttgggtcc cccgtcttgg cgttaccctg
     6001 cgcgaaaagc gagccgcaga cgatcaaggc agtcaccaca aggacggacc gtttcatttc
     6061 cttcccccgc tcagggtagt aacgcaggaa ccgccgggca agcgttgacg cgcctcgcct
     6121 tttgggctat tccgaccagg tgacctcccg aaagctcacg cccggagaga tctcctccag
     6181 cgtcgagaag atccgaaaga agtacgatca gtacgtcacg aagttctaca agtcccgctt
     6241 tctccggtcc gccttcgagg accgatacct caaggcgctt cgcgcgggcg tggacatctc
     6301 ctcattcctg ctggccgaga tcagcgccat cgaggagctg ctccagcggg aggaggcgcg
     6361 cgtggcggcc gccccctcca agccggctcc gccctccgca ccaacgcccg ggttcgccga
     6421 ccgcgtgctg gaagagaacc gcaagcggat caccgggtac ccggaggtct ccttccaccc
     6481 ggacgccagt gaagaggtgc ggaggctcct gggagcgctg gtggcgctgc acggagacac
     6541 gtggcagagg ctggggcatg ccctgcgcaa gaccatgtac tccatgtctt cctcggagat
     6601 gatccgcctg gactcggatc tgcgctccct ggcggcggga gatctggaca ctcctcccca
     6661 ggccttgctc cagctcgtgg tccagctcaa gaggttcccg cggaactacg cggcggtgga
     6721 gcgggaggag cgcgactata tgctggcggc ggcgttcttc ctgaacgacc tgcacggggc
     6781 gctggaccag gtgaaacggc tgtacacgga gatgaccgag gaggaccggc gctttctgga
     6841 cgagaccatg acccacgtct gggagatcgt gagcgacttc cggctcaagg agctgaagcg
     6901 ccgcgaccat tccgagacac gctgagggat tgctcccggc ttccaggcgg gtatatattt
     6961 gagcaccgga acagggggtc gaggatgtct gaggaagtag cagtcaccaa gagcaatttc
     7021 gacgcggaag tgatcaagtc cgaggtgccc gtggtggctg atttctgggc ggaatggtgc
     7081 ggcccgtgcc gcatgattgc gccggtgctc aagcagcttg cccaggagta caaggaccgc
     7141 atcaagattg ccaagatcga cgtggacaag gaaggcgagc tggccgagaa atacaacatc
     7201 gtcagcatcc cgactatcct ggttttcaac aagggcgaag tggtgaagca gcagatcggc
     7261 gccgtacccc gccctatcct ggaaaagatg atcaaggacg tggtggcgta ggagacccct
     7321 tcggccccgc cgcgttctcc gccgtcatag gaacgtatac ctcgtcactg ttctccgacc
     7381 cgaagctgga agcccgcgtg ggaacgatgt gccagtccgc ggccgtgttg gtgtccgtgg
     7441 aggcggagga gcgaccgagg gagcgggtgg ccgtggagcc ttccggactg accgcgtctt
     7501 ccggactgac cttctgagcc gccgccgtcc aggcgccctg tccggccagc tcctccgccc
     7561 accccagcat ctggtccgag ccgaagcctc gatacagctc gtccgacgtt gcggtccggt
     7621 tgctgtacag gatggcatcc aggatggcgc cgccgggacg gtcatacagg gtcagggcgc
     7681 cgttgttccc gggcaatccc gttccgccgg ccagccacac atccaccgcg gtctgcgaag
     7741 cgtcctgacc gccggacgct gagaggttgt ccgtctcatt cacctccacc ggatctcccg
     7801 ttggtcggca atggacgagg atgaaggctc ctttgtggat ggcgaacaaa gggaacacga
     7861 gccgttgttg gtagttgccc tgcgttccca cgcagaagac cacgccaccc atgtccccgt
     7921 ccgtcagggc cttgagctcc accaggtccg ggtggttgcc ggat
//
LOCUS       DASRZS010000465         4140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1409559, whole genome shotgun sequence.
ACCESSION   DASRZS010000465 DASRZS010000000
VERSION     DASRZS010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409559"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFH83_11375"
     CDS             <1..453
                     /locus_tag="VFH83_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487014.1"
                     /translation="TRGAMSGRERVLAAMRGQPVDYVPCAPYVNTLTAVHQAGRVSAY
                     TMYSGIMPILDYLSGLEFDCISALDIAFDEPDLGLIAGTLHRMSFWTGPSNTFHMYAG
                     PDVVRKAVRDVFSAFRKNGLIISAASSVHPMMPWENTLAMLEEWRRLR"
     gene            532..1629
                     /locus_tag="VFH83_11380"
     CDS             532..1629
                     /locus_tag="VFH83_11380"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6487015.1"
                     /translation="MKAAKIRIGVAGLGRIGWPFHCAQIAQHSQYEFVAVQDAAPERL
                     EEARKVYGVHGYRDFSEMLAESRLDAVAIATPTPFHRPMATEALRAGCHVMLEKPMAP
                     NATDAAAIVQEAARRRRVLTVYQPRRADAYFQHLKRMVDAGLVGQVYHVHVGMYQYVI
                     RNDWQALRKFGGGMLNNYGAHALDQVLQLIGYEVKRVFCNLRLVASLGNAEDVVKIVL
                     ETRDHAIGEVDINQASAIVPFTLDVWGTRGAISLSQDTTQFRVRSFPAGGARQSGLDP
                     ALASAGRQYPRGQIRWRESVVPVDLRLAVDVYEDLARAIRTGARPFVRPEEPLALMRL
                     IDRCREESGGIVSTSLFPRGGARRPPKRRAS"
     gene            1825..2313
                     /locus_tag="VFH83_11385"
     CDS             1825..2313
                     /locus_tag="VFH83_11385"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6487016.1"
                     /translation="MMRPYQPQDLPWVMDVANRAWRPINAAYRAAYGEKLFQRIVPNE
                     RFRKGLEMEGVCRDSPQNVFLCEENGRRVGFLTILFDRERLVGEIGNNAVDPDCGLKG
                     IGQQMYRFALERFRAEGMEFAKVHTGLDEGHAPARRAYERAGFDISHSDITYFMDLER
                     RP"
     gene            2387..3394
                     /locus_tag="VFH83_11390"
     CDS             2387..3394
                     /locus_tag="VFH83_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HET6487017.1"
                     /translation="MAPRILYGLGQALYEKIFPPTAVKKIAAVCEVLPIPVTQRPNRE
                     WLVESARRAEIVVSGWDTPMVDAAVLAASPGLKLVAHAAGSIKPMMSPEAWQRGVRVT
                     GASSAIAIGVAEFCVAMMLTAAKRVYWFSQRTRQGHWNRTDEAYGPALEIYHQKVGII
                     GASQVGRHLIKLLQSFDCEVLVYDPYWTRERLQALGARKVDTVDEIFTECRVVSLNAP
                     TTKETEGMIRGRHFAMLREGSVFVNTARGILINQDEMVEQLRRGRFVACIDVTTPEPL
                     PVDHPLRTLPNVILTPHEAGAMAENLMRIGEFIADEVERYAAGAPLVGEVKQSQLDTI
                     A"
     gene            3430..>4140
                     /locus_tag="VFH83_11395"
     CDS             3430..>4140
                     /locus_tag="VFH83_11395"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET6487018.1"
                     /translation="MDTLSQKMLRKHVSVICSRIGSRMIGTDGDRRSAEYVEACFLEY
                     GLAVTSNWMGCDGWDLESTSLRVAGKPFPAVGNMYSPSASGRGPLVLCEPDLPDARFR
                     ELKGKVVGVYGDFNPFPLRLNAFARKAERAGASAVVVIDTNQTTSATKLIREAALKRL
                     PVMSVSLETGFRLARLEGKSAQCVVKTRRYKSETRDVIGILEGGSREICIEAHRDTAP
                     DTPGADDNGSGTVVLLELA"
BASE COUNT          713 a         1340 c         1437 g          650 t
ORIGIN      
        1 acccgtggtg cgatgagcgg cagggagcgc gtgcttgcgg ccatgcgagg gcagcccgtg
       61 gactacgtgc cctgcgctcc gtacgtgaac accctgaccg cggtccacca ggcgggcagg
      121 gtctccgcgt acaccatgta ctccgggatc atgcccatcc tcgactatct ctcgggcctg
      181 gagttcgact gcatctccgc gctggacatc gccttcgacg agccggactt gggcctcatc
      241 gctggcacgc tgcacaggat gagcttctgg accgggccca gcaacacgtt ccacatgtac
      301 gcgggacccg acgtggtgcg caaggcggtg cgggacgtgt tctccgcctt ccgcaagaac
      361 gggctcatca tctccgccgc ctcttccgtg caccccatga tgccgtggga gaataccctg
      421 gcgatgctgg aggagtggcg ccggctgcgg tagcgcaggg ggcgcgctcg cagcgaaccg
      481 cgttgaccac gcccaaggta cggagtacgg tgcgtgcgaa agcgaggcag catgaaagcc
      541 gcgaagatcc ggatcggcgt ggcagggctg ggacgcatcg gatggccgtt ccactgcgcg
      601 cagatcgctc aacactccca gtacgagttc gtggcggtgc aggacgccgc ccccgagcgt
      661 ctggaggaag cgcgcaaggt ctacggggta cacggctatc gcgatttctc cgaaatgctc
      721 gccgagagcc gtctggacgc cgtggccatc gccacgccca cccccttcca tcggcccatg
      781 gccaccgagg ctctccgcgc cggctgccat gtcatgctgg agaagccgat ggcgccgaat
      841 gccacggatg cggcggccat tgtccaggag gcagctcgaa ggcggcgtgt cctcaccgtg
      901 taccagcccc ggcgcgcgga tgcctacttc cagcacctca aacgcatggt ggacgccggc
      961 ctcgtcgggc aggtgtacca cgtgcacgtg gggatgtatc agtacgtgat ccgaaacgac
     1021 tggcaggcgc tgcggaagtt cggcggcggc atgctcaaca actacggggc gcatgcgctg
     1081 gaccaggtcc tccagctcat cggctacgag gtgaagcggg tgttctgcaa cctgcggctg
     1141 gtcgcgtcct tggggaacgc cgaggacgtg gtgaagatcg tcctggagac ccgtgaccat
     1201 gccatcggag aggtggacat caaccaggcg tccgccatcg tgccgttcac gttggacgtc
     1261 tggggcacgc gcggcgcgat ctcgctctcc caggatacga cccagttccg cgtgcggtcg
     1321 ttcccagccg gaggagcgcg ccagtcgggg ctggaccccg cgctggcgag cgcagggcgg
     1381 cagtatccgc gcggccagat ccgctggcgg gaatccgtcg tcccggtgga cctgcgcctg
     1441 gccgtcgacg tctacgagga cctggcgcgc gccatccgca ccggcgccag gccgttcgtg
     1501 cggcccgagg agccgctcgc tctcatgcgg ctcatcgatc gatgccggga ggagagcggg
     1561 ggaatcgtct ctacgtccct gttccctcga ggaggagcaa ggcgtcctcc caagcgtcgg
     1621 gcgtcttgaa agacgccggg gccccggcgg aatacgcgct gcggccggtg aagccggtgg
     1681 tccccaccgt gtatcgccgt accaactccc actgggtcgt gcccatccag aagaccggct
     1741 ccccgcgctg gtccacgaag gagcggccat ccgcgctgat cccgagggta tgcatcgggt
     1801 caagaatacc cgtaccatgg caccatgatg agaccctacc agccccagga cctgccgtgg
     1861 gtaatggacg tggccaaccg ggcatggaga ccgatcaacg ccgcctaccg ggcggcctac
     1921 ggcgagaagc tcttccagcg catcgtgccc aacgagcgct tccgaaaggg tctggagatg
     1981 gagggagtct gcagggacag tccccagaac gtcttccttt gtgaggagaa cggccgcagg
     2041 gtagggttcc tcaccatcct gttcgatcgc gagcggctgg taggggagat cggcaacaac
     2101 gccgtggatc ccgactgtgg cctcaagggc atcgggcagc agatgtaccg cttcgctctg
     2161 gagcgattcc gggcggaggg tatggagttc gccaaggtcc acaccggcct ggacgaggga
     2221 cacgcgccgg cgcgccgcgc gtacgaacgg gccgggttcg acatcagcca ctcggacatc
     2281 acgtatttca tggatctgga gcggcggccg taggtcgctc tccaaggcgc cgcggtagac
     2341 cagccccggt ccgatcggta tcatcccccg ccagaggaga accatcatgg ccccaaggat
     2401 tctgtacggt ctgggacagg cgttgtacga gaagatcttt cccccaaccg cggtgaagaa
     2461 gatcgcggct gtctgcgagg tgctgccgat ccccgtcacc cagcggccca accgggagtg
     2521 gctcgtggag agcgcgcgca gggcggagat cgtcgtgagc gggtgggata cccccatggt
     2581 ggacgccgcg gtcctggccg cctcgccggg gctcaagctc gttgcccacg cggccgggtc
     2641 gatcaagccg atgatgagcc cggaggcctg gcagcgggga gtccgcgtga ccggcgcctc
     2701 cagcgccatc gccatcggcg tggccgagtt ctgcgttgcc atgatgctga cggccgcgaa
     2761 gcgcgtgtac tggttctccc agcggactcg acaggggcac tggaaccgga ccgacgaggc
     2821 ctatggcccg gccctggaga tctatcacca gaaggtcggc atcattggcg cgagccaggt
     2881 aggtcggcac ctcatcaagc tcctccagag cttcgactgt gaagtcctgg tctacgaccc
     2941 gtactggacg cgggagcgtc tccaggcgct gggcgcccgg aaggtggaca ccgtggacga
     3001 gatcttcacg gagtgccgcg tggtgagcct caacgccccg acgaccaagg agacggaggg
     3061 catgatccgc ggccgtcact tcgccatgct ccgggagggc tcggtgttcg tgaacacggc
     3121 tcgggggatc ctcatcaacc aggacgagat ggtggagcag ctccgccgcg ggaggttcgt
     3181 cgcttgcatc gacgtcacca cccccgagcc gcttcccgtc gatcacccgc tgcgcacgct
     3241 ccccaacgtg atccttacgc cccacgaggc tggagccatg gcggagaacc tcatgaggat
     3301 cggcgagttc atcgcggatg aagtggagcg gtacgccgcc ggcgcgccgc tggtgggaga
     3361 ggtcaagcag agccagctcg ataccatcgc ctgacggtgt ccgggcgcgg atggggtaga
     3421 ggaggagccg tggacacgct gtcgcagaag atgctgagaa agcacgtttc cgtcatctgc
     3481 tcgaggatcg ggtcccgcat gatcggtacc gacggagacc gccgctcggc ggagtacgtc
     3541 gaggcatgct tcctcgagta cgggctggcc gtcaccagca actggatggg gtgcgacggc
     3601 tgggacctgg agtccacgag cctccgcgtg gcaggcaagc ccttcccggc ggtcggcaat
     3661 atgtattccc cctcggcttc cggccgcggc ccgctggtcc tgtgcgagcc ggacctgccc
     3721 gacgcccggt tccgcgagct caaggggaag gtggtcgggg tgtacgggga tttcaacccg
     3781 ttcccgctgc gcctgaatgc gtttgcccga aaggccgaga gggcgggcgc gtccgccgtc
     3841 gtggtcatcg ataccaacca gaccacgagc gccacgaagc tgatccgcga agccgcgctc
     3901 aagcgtctcc cggtgatgtc cgtgagcctg gagaccgggt tccgcctggc caggctggaa
     3961 ggcaagagcg cgcaatgcgt cgtgaagacg cggcggtaca agagcgagac ccgagacgtg
     4021 atcgggatcc tggagggggg gtcccgggag atctgcatcg aggcgcatcg ggacacggcg
     4081 ccggataccc cgggcgcgga cgacaacggg agcggaaccg tggtgctgct ggagctggcc
//
LOCUS       DASRZS010000466         4548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_249968, whole genome shotgun sequence.
ACCESSION   DASRZS010000466 DASRZS010000000
VERSION     DASRZS010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249968"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VFH83_11400"
     CDS             complement(<1..1034)
                     /locus_tag="VFH83_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6487019.1"
                     /translation="MSTRRDRVLSALAFREPGVVPKDLGGMRSTGISAFAYPRLRAAL
                     GLPPRLPRMYDTSQMLALPELDVLDALDCDVIHVTLDEVTNAFDEPKRWKPYDHNGRL
                     PSLVMNPGAYAVEPDGTIVQTLGGSPARMVPGAHVFDHEHGGEVLDLEAELTEPDYAD
                     VERRLMSYVFSEERARSIGAYCRRVRESTDRAVMLNGLQADLGFPGGMAAWSMTCLLH
                     PDWVRRVHELKVRFAEAQIAALAPHLRGFVDLIVFAADDQGTQNGPILPPDVFTELYV
                     PYYRRMNDALHRALPGVFSFLHCCGAVYDLLDGIAEAGFDVLNPVQWCAGHHTYKAWK
                     ERCGTRLALWG"
     gene            complement(1088..2506)
                     /locus_tag="VFH83_11405"
     CDS             complement(1088..2506)
                     /locus_tag="VFH83_11405"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6487020.1"
                     /translation="MPQGVSISLESREYRWRWWALVGASLAVFMAALDNNVVNVALPI
                     LARNFQVDRQIRWVALAYILPGTALLGAFGALSDALGRRRITLLGVSLFLLGSVLCGT
                     ARSLEQMILYRIIQGTGAAAVGSAVLAIATVNFAPEERGRAMSVIGLIAPLGAVVGPS
                     LGGLLIGLLGWPSIFYINVPVGLVALVLIYRLLPKDTSRMTKGFDVPGASLFTAALVL
                     LILGLNPGDGRMRTPEVLMLVGAAAALGGLLLVERRARNPLVPLSLVSRRHFTIPAAG
                     IMTFAIVGTALGFILPFFLEGVQGLGPERTGLTLLFFPLAMAVTSQIGGRLTDRFNPR
                     LPAAVGAAISLIGVALFLPMSAAWRPLDVALRGAVVGFGFGFFISPSAVAVMAAAPRD
                     HIGVGGALLNTARFLGFALGPTLATVFWTPGVQGVGSLTSMRTVVLVVVVVQVLTLAT
                     VLAYRVSREERQAKPSVETTAA"
     gene            complement(2586..3068)
                     /locus_tag="VFH83_11410"
     CDS             complement(2586..3068)
                     /locus_tag="VFH83_11410"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6487021.1"
                     /translation="MVRAMRQPPKSETTLTAPPSLGGSDDSLSHWVKLAYFTMRREME
                     DSLRAQGLTLTQWRALGALLHTPGTTHSDLVRLLEIEPPSVTSLVNGMLKHGWVRQER
                     SATDARVKRLYLTPRGRHTIQSARVACAPVERRMEASLPEEDRASLKRLLRDVIDGLQ
                     "
     gene            complement(3181..4332)
                     /locus_tag="VFH83_11415"
     CDS             complement(3181..4332)
                     /locus_tag="VFH83_11415"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HET6487022.1"
                     /translation="MKTLGQVEAGQISVRQAAVKMRMSYRQARRVWKRYREGGDGAIV
                     HGARGQAPNNQIPLELKRQILERYREVYQGFGPTLACEKLVEEDGLRAISRETLRLWL
                     LEEKLWERHRRRGAYRKRRPPRAHFGELVQLDGSFHRWFGEQHNECCLMSMVDDATGT
                     TLVRMGEQETTEAAMRLLWAWMERYGIPESLYCDRKNVYLLPKGEQPSLEEQLEGKLP
                     KSAFGVACEKLGIQIITAHSPQAKGRVERKHGVFQDRFVKELRLRGATRIEEANLILQ
                     GGFTEKLNRKFAHPALEPEDFHVALRPSQHLDDILCFEEVRSVGLDWVVRHENRFYQL
                     LRGRQPLPRAKSKVTVRRRLDGSTQILHRGKELAYEEFDPVSKERGMAV"
BASE COUNT          827 a         1531 c         1459 g          731 t
ORIGIN      
        1 ccccatagcg ccagccgagt cccgcaccgc tccttccagg ccttgtacgt gtggtggccg
       61 gcgcaccact gcacggggtt gagcacgtcg aaccccgcct cggcaatgcc gtccaggaga
      121 tcgtagaccg cgccgcagca atggaggaac gagaacacgc cgggcaacgc gcggtgcagg
      181 gcgtcattca tccgtcggta atagggaaca tagagctccg taaacacgtc cggcggcagg
      241 atggggccgt tctgggtgcc ctggtcatcc gcggcgaaga cgatcaggtc cacgaacccc
      301 cgaagatgag gcgccagcgc ggcgatctgc gcctcggcaa atctcacctt cagctcgtgg
      361 acccttcgga cccagtcggg atgcagcaga cacgtcatgg accatgccgc cattccgccg
      421 gggaacccga gatccgcctg cagcccgttc agcatcacgg cccggtccgt gctctcccgc
      481 acccggcggc aatacgcccc gatggagcgc gcccgttcct cgctgaaaac gtacgacatc
      541 aagcgccgct cgacgtcggc gtagtccggc tccgtgagct ccgcttccag gtccaggact
      601 tccccgccgt gctcgtggtc gaagacatga gcgccgggca ccatgcgcgc cggggatccg
      661 cccagggtct ggacaatggt cccgtccggc tccacggcat acgcgcccgg gttcatcacc
      721 agggacggca acctgccgtt gtggtcgtag ggcttccagc gcttcggctc gtcaaaggcg
      781 ttggtgacct cgtccagggt gacgtggatc acgtcgcagt ccaacgcgtc cagcacatcg
      841 agctcgggca gggccagcat ctgggaggtg tcgtacatcc gtggcagccg aggcgggaga
      901 cccagggcgg cccgcagcct gggataggca aacgcgctga tcccggtgga ccgcatgccg
      961 ccgaggtcct tgggcaccac gcccggctcg cggaacgcca gcgcggacag gacacggtct
     1021 cttcgggtac tcatggtgat ctcctcgtgg ttgagggtcc cagagtaccc gagagacgct
     1081 tgggacatca agccgcggtg gtctccacgc tcggcttggc ctgccgctct tcgcggctca
     1141 cgcggtatgc caggaccgtc gcgagcgtga gcacctgcac caccaccacg acgagcacca
     1201 ccgtcctcat ggaggtcagg ctgcccacgc cctggacgcc gggcgtccag aagaccgtgg
     1261 ccagcgtcgg acccagggcg aagccgagga accgagccgt attgaggagc gcgcccccca
     1321 caccgatgtg gtcccgcgga gccgcggcca tgaccgctac ggcgctcggg gagatgaaga
     1381 acccgaaccc gaatccgacc acggcccccc ggagcgccac gtccaaggga cgccacgcag
     1441 cgctcatggg aaggaacagg gccacgccta tcagggagat ggccgctccc acggcagccg
     1501 gaagccgggg gttgaaccga tccgtgagcc ggccgccgat ctgggaggtc acggccatgg
     1561 ccagcgggaa gaacaggagc gtgagcccgg tgcgctccgg acccagcccc tggacgcctt
     1621 ccaggaagaa cggcaggatg aatccgagcg cggtccccac gatggcgaag gtcatgatgc
     1681 cggcggccgg gatggtgaag tggcggcgcg acaccagaga aagcggcacc aggggattcc
     1741 ttgcccgtcg ctccaccagc agcaacccac ccagggcggc cgcggcgccg accagcatga
     1801 ggacttccgg cgtcctcatc cgcccatctc cgggattgag ccccaggatc agcagcacca
     1861 gcgccgcggt gaagagcgac gcgcctggca cgtcaaagcc cttcgtcatc cggctggtgt
     1921 ccttgggaag cagccggtag atcaggacaa gcgccaccag ccccacgggc acgttgatgt
     1981 agaagatcga gggccacccg agaaggccga tcaggaggcc gcccaggctc gggccgacca
     2041 cggctcccag cggggcgatc aggccgatca ccgacatggc ccgaccgcgc tcctccgggg
     2101 cgaagttcac cgtggcgatg gcaaggacgg ccgagccgac ggcggccgcg cccgtgccct
     2161 ggatgatgcg gtacaggatc atctgctcga ggctccgcgc ggtcccgcac aggacgcttc
     2221 ccaacaggaa gagcgagaca ccgagcaggg tgatgcggcg gcggccgagc gcgtcggaca
     2281 gcgcgccaaa ggcgcccagg agcgccgtgc cgggaaggat gtacgccagg gccacccagc
     2341 ggatctggcg atccacctgg aagttccggg ccaggatggg aagggccacg ttgaccacgt
     2401 tgttgtccag cgcggccatg aagacggcga gggaggcgcc caccagggcc caccagcgcc
     2461 agcggtactc acgggattcc agcgatatcg aaacgccttg cggcatgaac cactcctctc
     2521 gttcgaggag gggaacttac ttagagttca aactaaaggt caagaggttt atttcgatct
     2581 ctaacttact ggagcccgtc gatcacgtcc cgtaggagcc gtttcaggga ggcgcggtcc
     2641 tcctccggga gggacgcctc catgcgccgc tccacggggg cgcaggcaac gcgggcgctc
     2701 tggatggtgt ggcggccgcg gggggtgagg tagaggcgct tgacccgggc gtccgtggcg
     2761 gagcgctcct ggcggaccca gccgtgcttg agcatgccgt tgaccaggct ggtcacgctg
     2821 ggcggctcga tctccaacag gcgaaccagg tcggagtggg tggtcccggg cgtgtgcagg
     2881 agcgcgccca gggcccgcca ctgggtaagg gtgagcccct gcgctcgaag ggagtcttcc
     2941 atctctctgc gcatggtgaa gtaggcgagc ttcacccagt ggctgagcga atcgtcgctc
     3001 ccgccgagtg agggaggggc tgtcagggtc gtctcggact tcgggggctg ccgcatagct
     3061 ctgaccacag ggctcctgag tgagcgtaac cacgcctcgg acaatcgtca agcgacgggg
     3121 cttgctctgt caaggcaaaa cagaaatgtc cccatattca gcaaagcaga aatgtcccct
     3181 tcacaccgcc atccctcgtt ctttgctcac cgggtcgaac tcctcgtagg cgagctcttt
     3241 gccccggtgc aggatctgcg tcgagccgtc caaccgcctc ctgacggtga ccttgctctt
     3301 ggctcggggc aacggctggc gaccccgaag cagctggtag aagcggttct catggcgcac
     3361 tacccagtcc aggcccacgc tacgcacctc ttcgaagcac aggatgtcgt ccaggtgctg
     3421 gctgggtcgt agcgcgacgt gaaagtcctc gggttccaac gcagggtgag cgaacttgcg
     3481 attcagcttc tcggtgaacc ctccctgcag gattaggttc gcctcctcga tcctcgtggc
     3541 ccctcgcaaa cgcaactctt tgacgaagcg atcctgaaac accccatgct tgcgctccac
     3601 tcgtcccttg gcttgggggg agtgggccgt gatgatctga atgcccagct tctcacaggc
     3661 caccccgaag gcgctcttgg gtagcttgcc ctccagttgt tcctccagcg acggctgctc
     3721 gcccttaggc aggaggtaca cgttctttcg gtcacagtac aggctctcgg ggatgccgta
     3781 gcgttccatc caggcccata aaaggcgcat cgccgcctcg gtggtctcct gctcacccat
     3841 gcgcaccagg gtggtcccgg tggcgtcgtc caccatgctc atcaggcagc attcgttgtg
     3901 ctgttcacca aaccagcggt gaaacgaccc atccagctgc accagttccc cgaaatgcgc
     3961 ccgcggcggg cgacgcttgc ggtaggcacc acggcggcga tggcgctccc aaagcttctc
     4021 ctccagcagc cacagccgca acgtctcacg gctgatcgcc cgtaatccgt cctcctccac
     4081 caacttctca caggccaacg tcgggccaaa gccctggtag acctctcgat agcgctccaa
     4141 gatctgccgc ttgagctcca gcgggatctg gttgttggga gcctgcccgc gcgccccatg
     4201 gacaatcgcc ccgtccccgc cttctcgata gcgcttccac acccgcctgg cctggcggta
     4261 gctcatgcgc atcttcaccg cggcctgccg aacggagatc tgaccggcct ccacctgccc
     4321 gagtgtcttc aacctcgaac tctccctcat ggacatctgc agcgtcccct ctgtcatcgt
     4381 gggcaacctc gtcttggtgt ggttgcccca attctagcgg ggacatttct gctttgccca
     4441 aagaggacat tactgctttg ctacaacagg caaagggatt caggtgttgc atatacttgc
     4501 agggtgggac gtgcgaagga gggaagcatg agggagcgga agacggga
//
LOCUS       DASRZS010000467         4855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2945608, whole genome shotgun sequence.
ACCESSION   DASRZS010000467 DASRZS010000000
VERSION     DASRZS010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2945608"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..339
                     /locus_tag="VFH83_11420"
     CDS             61..339
                     /locus_tag="VFH83_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487023.1"
                     /translation="MWFYYFRVGLTYVTIGVAAAVFFFFVVKKPVLGRLWGALIVGLV
                     GSFLGGLIDQIFSRVIAYLSDVNSVNVFSALATSLLMIWLLSKASYPR"
     gene            349..546
                     /locus_tag="VFH83_11425"
     CDS             349..546
                     /locus_tag="VFH83_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HET6487024.1"
                     /translation="MDGTVRSRIAPGAKVTIVQKADQHSGRLTEGIVRDILTNSPTHP
                     HGIKVRLETGEVGRVKEILPS"
     gene            623..1051
                     /locus_tag="VFH83_11430"
     CDS             623..1051
                     /locus_tag="VFH83_11430"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HET6487025.1"
                     /translation="MKWIKAPEDLKTLIERLMQPIDCERRPMFGYPAYFVNKHLFAGL
                     FQDMLFVRLSPAQSMELRKDFPAVASLEPMPGRPMKDYTVIPAELRQDARKMARILKD
                     AATWCRSLPPKATTARKAAKAPKARTPGKPATRPAARKRT"
     gene            complement(1111..4047)
                     /locus_tag="VFH83_11435"
     CDS             complement(1111..4047)
                     /locus_tag="VFH83_11435"
                     /inference="COORDINATES: protein motif:HMM:NF015894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HET6487026.1"
                     /translation="MTVRGELKLEFDDPALEARVTITVREDGDELSPDAVLANLKKKG
                     VRAPADTEAVERAFRSLGRRKDESITFVAASGTAPVAPEREVVEFEDLPTPRRLALVG
                     PRILAQAAKPEGFRLREVKVRREKKVLKKPALPFLQPQEEVEVVVEKKLVREAVQIDP
                     AVLATGFAAKDALVARIRPGKPGKDGRDVFGRLVAGSRAERAEWLLCSGLTRAGAEVR
                     ATVTGFLRRGASWCDIVPFHDHELSVTAGPDGTACLLSFEPGDAVIPPPTGDEVLELA
                     GKLGFAAEGLLSASQIEALLRKSVSSAVGIENRPLSQTTDGYATVTVSEDRLRATLSL
                     RKGKGGGSPLTPGRVSDAIRESHVRRYNAETVRRDIQEFLKGPDTELSDYVLAEGKAP
                     GASQEGGLKWSVTLRTLSADRKPSAGNRDLSEPMASLREFPLESVESVCAIAAETPVV
                     RIAPGSVGSSGVDVFGAVIPGLRGETPVVRIFEGLELRRDTVFSTAEGLLEKGTAGAV
                     ILLRVRPHRDAELDVTISDDRMQGFLAFTPPLGTGRSIGAEEVRAAIGQAGIVRGVDE
                     ARLATALEAIARGQAVPKMRIAQGRKPALEPEKLVTFHVRLATGKAMALRSDGSADFR
                     AQDRITHVAKGAHIATLKPPPPESSDAWDVTGRALFPTGDASHWLTAGRGVSAVRQPD
                     GTLHFYAQADGEIIRDGSLIAVEQVHTVDDVDMATGNINFPGIVRVRGSVRAGFRILA
                     AGDVEIEETVEAAAVTSQRSILVGQGIKGDGKAAVRANKGIVAPFAEQALLRAAETIQ
                     LKGACLRCNVVCGKLILDSEKGNLVGGEVRARGGIVVQNLGSPAGTRTLVSFGQDFLA
                     REEADHLLAEVSVLEKRLTEIDDRVKELQKSGKAGAAQDRMAIARIRVEKLHVVNGLE
                     ERRARLAELNLALEARIPSEIVVRGTFYPGAVIESHGKRWETLEEKHMITLAYDPKQD
                     RIVVRL"
     gene            4405..>4855
                     /locus_tag="VFH83_11440"
     CDS             4405..>4855
                     /locus_tag="VFH83_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-protection protein"
                     /protein_id="HET6487027.1"
                     /translation="MAHANTEEVLTFSTPGAWARWLRANGSSSAGVWLRLARKGSRVK
                     TLSYQEALEEALCQGWVDGQKKSLDDEAWLQRFSRRGPRSIWSEVNRTKALRLIRAGR
                     MEAAGLAAVQAAKASGRWEKAYASQAKAKVPGDLAAALKADPEAQAFF"
BASE COUNT          808 a         1655 c         1508 g          884 t
ORIGIN      
        1 cagcgccggt ctacctgggg tatgcaccgc tgcccaattt ctagtatagg atgaggttgt
       61 atgtggtttt actacttcag ggtcggattg acgtacgtca ccatcggggt ggccgccgcg
      121 gtgttcttct tcttcgtggt gaagaagccc gtgctgggaa ggctgtgggg cgcgctcatc
      181 gtcgggctgg tgggatcgtt tcttggcggc ctgattgacc agatcttctc cagggtgatc
      241 gcgtacctct cggacgtgaa ctcggtaaac gtgttctcag ccctggccac gtcgctactg
      301 atgatctggc tgctgtccaa ggccagttac ccacgatagg ggcgcgccat ggacggtacg
      361 gtgcgctcgc gtatcgctcc cggcgcgaag gtcacgattg tccagaaggc ggaccagcac
      421 tcggggaggc tcacggaggg gatcgtcagg gacattctca ccaactcccc cacgcatccc
      481 cacggcatca aggtgcgcct ggagaccggt gaggtgggcc gcgtcaagga gatcctgcct
      541 tcgtagggcg gcccgccccg gcggactttg ccgctcgcgc ccggccggta gtactattcc
      601 cccaccacgg agggggatgc ccatgaagtg gatcaaggct cccgaagacc tcaagactct
      661 catcgagagg ctgatgcagc ccattgactg cgagcgacgg ccgatgttcg gataccccgc
      721 ctatttcgtg aacaagcacc tgttcgccgg cctgttccag gacatgttgt ttgtccgctt
      781 gtcccccgcg caatccatgg agctccgaaa ggactttccg gcagtcgcaa gcctggaacc
      841 gatgcccggc cggcccatga aggattacac ggtcattccc gcggaacttc ggcaggacgc
      901 caggaagatg gcaaggatcc tgaaggatgc ggcgacctgg tgtcggtccc ttcctcccaa
      961 ggccacaacc gctcgcaagg cggccaaggc ccccaaggcg cggacacccg ggaagcctgc
     1021 caccaggccg gctgcccgga agcggacctg accgcgccct tcgcgcgggg gacgtgaagc
     1081 cccggtccgc tccgccaggc cgcccgatct tcacagcctc accacgatcc ggtcctgctt
     1141 tggatcgtag gcgagggtga tcatgtgctt ttcctccagg gtctcccagc gcttcccgtg
     1201 gctttcgatc accgcgcccg ggtagaacgt gccgcgcacg acgatttcgg acggaatccg
     1261 cgcttcgagg gcgaggttca gttccgcgag ccgcgcccgg cgctcctcga gcccgttcac
     1321 cacgtgcagc ttctcgaccc ggatccgggc aatcgccatg cgatcctggg ccgctcccgc
     1381 cttgccggac ttctgcaact cctttacccg gtcatcgatc tcggtgagcc tcttctcgag
     1441 caccgagact tccgccagca ggtgatccgc ttcctcgcgc gccaggaaat cctgaccgaa
     1501 cgacaccagc gtgcgcgtcc cggccgggga gcccaggttc tggacgacga tcccgccgcg
     1561 ggcccgcacc tcacctccca ccaggttgcc cttctccgag tcgaggatca gcttgccgca
     1621 cacgacgttg cagcggaggc aggctccctt gagctggatc gtctccgccg cgcgcaggag
     1681 cgcctgctcg gcgaacggag cgacgatgcc cttgttcgcc cggaccgccg ccttgccgtc
     1741 tcccttgatc ccctggccga cgagaatcga tcgctgtgac gtgacggcag cagcctccac
     1801 ggtctcctcg atctcgacgt cccccgcggc gaggatccgg aaaccggccc gtacggagcc
     1861 ccgcactcga acgatcccgg gaaagttgat gttgccggtt gccatgtcca cgtcgtccac
     1921 ggtgtggacc tgttccacgg cgatcaggga gccgtcacgg atgatctccc cgtccgcctg
     1981 ggcgtagaag tgcagggtgc cgtcgggctg gcgcacggcg gagacgccgc ggcccgccgt
     2041 cagccaatgg gaggcgtccc cggtgggaaa aagcgcgcgg ccggtgacat cccaggcatc
     2101 tgaggattcc ggtggcgggg gcttcagggt tgcgatatgt gctccctttg ccacgtgggt
     2161 gatcctgtcc tgggcccgga aatcagcgct tccgtcactt cgaagcgcca tcgctttccc
     2221 ggttgccagt cggacgtgga acgtaacgag cttctccggt tccaatgccg gctttcggcc
     2281 ctgcgcaata cgcatctttg ggacggcttg gccgcgcgcg atcgcctcca gcgcggttgc
     2341 gagcctggcc tcgtccactc cacgcacgat gccggcctgc ccgatcgctg cgcgcacctc
     2401 ttcggcgcct atcgaacgcc ccgtgccgag ggggggagtg aaggcgagga acccctgcat
     2461 gcggtcatcc gagatcgtga catccagttc cgcgtcacgg tggggccgta ccctcaggag
     2521 gatgaccgct ccggcggtcc ccttttccaa cagcccctct gccgtggaga acactgtatc
     2581 ccgcctcagc tccaggcctt cgaagatcct gaccaccggc gtctctccac gcagccccgg
     2641 gatgacggct ccgaagacgt cgactcccga cgaccccacc gaaccgggtg cgattcgcac
     2701 taccggggtc tcggcggcga tggcgcacac cgactctacg ctctccaggg ggaactcccg
     2761 cagcgacgcc atgggctccg aaaggtcgcg gttccccgcg gaaggcttgc ggtccgcgga
     2821 cagggtccgg agtgtgacgg accacttcag gccgccttcc tggctcgccc ccggcgcttt
     2881 gccttccgcg agcacatagt cggagagctc cgtgtcagga cccttgagga actcctggat
     2941 gtcccgccgc acggtctcgg cgttgtaacg gcgtacgtgg ctttcccgga ttgcgtccga
     3001 tactcgaccg ggagtcagtg gggacccccc tccctttcct tttcgcagcg aaagcgtcgc
     3061 ccgcagccgg tcctcagaaa cggtcaccgt tgcgtagccg tcggtcgtct gggagagcgg
     3121 ccggttttcg attccaacgg cggagctcac gcttttccgc agcagcgcct ctatttgcga
     3181 ggcggaaaga aggccctcgg ctgcaaaccc cagctttccg gccagttcga gaacctcgtc
     3241 ccccgtgggg ggcggaatga ccgcgtctcc gggctcgaac gacagcaggc acgccgtgcc
     3301 atccggaccc gcggtcacgg aaagttcgtg gtcgtggaag ggtacaatgt cgcaccacga
     3361 cgcaccgcgg cggaggaagc cagtgaccgt tgcccgaacc tcggctcccg cgcgcgtaag
     3421 ccccgagcat agcagccact ccgcgcgttc ggcgcgggag ccagccacga gcctgccgaa
     3481 aacgtctctt ccgtcctttc caggcttccc gggccggatc ctggctacga gcgcatcctt
     3541 ggcggcgaac ccggtggcca gcacggccgg gtcgatctgc accgcttccc gcaccagctt
     3601 cttttccacg accacctcta cttcttcctg gggctgaagg aaggggaggg caggcttttt
     3661 cagtactttc ttctcccttc gcaccttgac ctctctcagg cgaaagccct cgggcttcgc
     3721 ggcctgagca agtatcctgg ggccgacgag ggcaaggcgc cgaggtgttg gaaggtcctc
     3781 gaactcgacg acctcccgct ccggtgcgac cggtgccgtg ccgcttgccg caacgaacgt
     3841 gatcgactcg tccttcctcc ggccgaggct ccggaaagcc cgctcaaccg cctcggtgtc
     3901 cgcgggtgca cggacgccct tcttcttcaa gttcgcaagc acggcatccg gggacagctc
     3961 gtcaccgtcc tccctcacgg tgattgttac gcgcgcctca agcgccgggt cgtcgaactc
     4021 cagcttcaac tcacctctga cagtcatcgt ccgtcctcga gcatttccag gtaggggtca
     4081 tcttcaagca tattccgaag tcgccccccg cacaaacaaa tccaaccctt cgttgcgggt
     4141 gttgacgcct aaacgggacg cttgggcccc accgatggca cttccgataa tacatattct
     4201 gtctaccgtc aaggatcggg caggcggcac tcgaaagcct cgtcctccct gggctccaag
     4261 caacgggcgg ccaagtgata ttcctcgagc atgtgcacga atcagtacaa catgaagtgc
     4321 tttgggtcac gcgcgagcca gttgcacgcc cttgatgcca gggcggttga cgccccagtc
     4381 tcctgttgtc gactttcgtt gtcaatggct cacgcaaaca cggaagaagt gctcacgttc
     4441 agcacgccgg gcgcctgggc gcgctggcta agggccaacg gctcaagctc ggcgggcgtc
     4501 tggctgaggc tcgccaggaa aggttcacgg gtgaagaccc tctcctacca ggaagccctg
     4561 gaggaggcac tgtgccaggg atgggtagac ggccagaaga aaagtctcga cgatgaggcc
     4621 tggctgcagc gcttctcacg gcgcgggcct cggagcatat ggtcggaggt caatcgcacc
     4681 aaggcgctcc ggctcatcag ggccggacgc atggaggccg cgggccttgc agccgtgcaa
     4741 gccgccaagg ccagcgggag gtgggaaaag gcgtacgcgt cgcaggccaa ggcgaaggtc
     4801 cccggagacc tggccgctgc gctcaaggcg gaccctgagg cccaggcgtt ttttt
//
LOCUS       DASRZS010000468         3302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2563368, whole genome shotgun sequence.
ACCESSION   DASRZS010000468 DASRZS010000000
VERSION     DASRZS010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2563368"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1206
                     /locus_tag="VFH83_11445"
     CDS             22..1206
                     /locus_tag="VFH83_11445"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6487028.1"
                     /translation="MTSRQRILSAVSCGPVDHVPLYLRFWPMGGKDTIPFDWKDQVHR
                     AEYLLFRGLDDTLLLQPPLGYIEDYRAELLPSVTATVRLEPAAAGEYPVLHKEYRTAA
                     GVLRHSVRKTDDWIHGNDVYLFSDYNVSRATRHAVQTEEDLGVLPYVLAEPSAEQVEA
                     FAAEADRLRRHSRRLGVALDGGWVALGDAAVMLCGMERVLLAQMEEPEFLRRLMDILL
                     TWELRRVERLLEQGVDMIVHMAWYEGTDFWTPDNWRRFVRPGLSRLVERAHAAGAKYR
                     YIITKGLAPLLADIRDAGVDCVMGVDPVQDRLDLAAVKEQSAGRLCLMGGVNSPIMLS
                     QWTEAEIDAAVSTAVRVLGRNGGFILYPVDAVFSDVAWDRVEQLIRCWERHRDRFDGK
                     RP"
     gene            1203..2309
                     /locus_tag="VFH83_11450"
     CDS             1203..2309
                     /locus_tag="VFH83_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6487029.1"
                     /translation="MSRREAWKAVMEYGELSEVPALWWDPWDETRERWTTEGMPAGVD
                     PHAALGTIRFWINLEVDLGLRPGFPQECLEETADSVVRRLADGTVQRSWKHGSSIPQY
                     VDHALKTARDWPDFKRRLRPDAGRIPPDLADRARALVAADDLVLIDCGSLMGWIRNWM
                     GLEGMSYLLFDDPDAFRDMVATIADLACWAIDQTIPRLPVLPDAGILWEDMCGKNGPL
                     IDPELVSRYVAPHYRRIRQRLDRHGVHLLGVDCDGDITGLLPVWLESGVNLHMPVEIG
                     TWKADPMALRRRHGRELRMVGGIDKRELEKGPAAIDREVARRMPLVRDGGFIPMPDHY
                     ITPQTSLADFRYYLSRIGGIRIDAPGTRPEVTRA"
     gene            2306..>3302
                     /locus_tag="VFH83_11455"
     CDS             2306..>3302
                     /locus_tag="VFH83_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET6487030.1"
                     /translation="MKMDFRKHDSHSEQANILRIVHRSRRITRRQIVDLTGFSQAKIS
                     IVINELKSRGLLAELEETSSSGGRKAKLLQLSSSSSHVIGVEMGGYEVKAALIDLAGN
                     LLCSRKMPAPSDITNPDQVLKIIAEFIQDFRATACPAGSHPLGVGIALSGITDRATGE
                     CVYFRNQRAWEGYQVGRKMEERLHLPCIVDDSSRMMAVAEWLYGVCRGVDSFVLISIG
                     MGTGCGIFVDGEIFRGTNGFGGELGHMVIKENGPRCVCGNFGCVESFISGYALERRLR
                     EALQDNVYTAMMHLEDRSAKEIVNHALQGDKLAYSIVTDAAEHLGIGIANVINIFN"
BASE COUNT          560 a         1064 c         1131 g          547 t
ORIGIN      
        1 acgagggggg catctagccg aatgacctcg cgccagagga tcctgagcgc cgtctcctgc
       61 ggacccgtgg accacgtccc gctgtacctg cgcttctggc ccatgggcgg aaaggacacg
      121 atccctttcg actggaaaga ccaggtgcac cgggcggagt acctgctttt ccgggggttg
      181 gacgacacgc tcctgctcca gccccccctg ggatacatcg aggactaccg ggcggagctt
      241 ctgccgtccg tcaccgccac ggtgcgcctg gaaccggccg cggccggaga ataccccgtg
      301 ctgcacaagg agtaccgcac cgcggccggg gtcttgcgcc attccgttcg caagacggac
      361 gactggatcc atggcaacga cgtgtatctc ttcagcgact acaacgtctc tcgcgccacc
      421 cgacacgccg tccagaccga ggaggacctt ggcgtcctgc catacgtgct cgccgagccc
      481 agcgcggagc aggtcgaggc ttttgccgcc gaggcggacc ggctccgccg ccattcccgc
      541 cggctgggcg tggccctgga cggcggatgg gtggcgctgg gggacgcggc ggtcatgctc
      601 tgcggcatgg aacgggtgct cctcgctcag atggaagagc ccgagttcct gcggcgcctg
      661 atggatatcc tgctcacctg ggagcttcgc cgcgtggaac gcctgctgga gcagggcgtg
      721 gacatgatcg tgcacatggc gtggtacgag ggtacggact tctggacgcc ggacaactgg
      781 cgccggttcg tccgccccgg gctttcgcgg ctggtagaga gggcccacgc tgccggagcg
      841 aagtaccgct acatcatcac caaagggctg gccccgctcc tggcggatat ccgggacgcc
      901 ggagtggatt gcgtgatggg cgtggatccc gtccaggacc ggctggacct tgccgccgtc
      961 aaggagcagt ccgccggacg gctctgtctc atggggggcg tcaattcacc catcatgctc
     1021 tcccagtgga ccgaggcgga gatagacgcg gccgtctcga cggccgtccg ggtcctggga
     1081 cgcaacggag gcttcattct ctaccccgtg gatgccgtgt tcagcgacgt ggcgtgggac
     1141 cgcgtggagc agctcatccg gtgctgggag cggcaccggg accgcttcga cgggaagcgt
     1201 ccatgagtcg ccgcgaggcg tggaaggccg tcatggagta cggcgagctc tcggaggtgc
     1261 cggcgctctg gtgggatccc tgggacgaga cccgggagcg ctggaccacc gagggcatgc
     1321 cggccggcgt ggatccccac gcggcgctcg gcaccatccg gttctggatc aacctggagg
     1381 tggaccttgg ccttcggccc ggctttccgc aggagtgcct ggaggagacc gccgactcgg
     1441 tggtccgtcg cctcgcggac ggcacggtgc agaggagctg gaagcatggc agctccatcc
     1501 cgcagtacgt ggaccacgcg ctgaagaccg cccgagactg gccggatttc aagaggcgcc
     1561 tgcgccccga cgccggccgc attccgccgg acctcgcgga tcgggcccgg gcgctcgtcg
     1621 ccgcggatga cctggtgctc atcgactgcg gctcgctcat gggctggatc cggaactgga
     1681 tgggcctgga aggcatgtcc tacctgctgt ttgacgaccc cgatgccttc cgcgacatgg
     1741 tcgcgaccat tgcggatctg gcctgctggg ccatcgacca gaccattccc cgcctgccgg
     1801 tcctccccga cgcgggcatc ctctgggagg acatgtgcgg caagaacggg ccgctcatcg
     1861 accccgagct ggtgagcaga tacgtggcgc cgcactatcg tcgcatccga cagaggctgg
     1921 accgtcacgg cgtccacctc ctgggggtgg actgcgacgg agacataacc gggctgcttc
     1981 ccgtgtggct cgagtccggc gtgaacctcc atatgcccgt ggagatcggg acctggaagg
     2041 cggacccaat ggcgcttcgc aggcgacacg gcagggagct gcgaatggtc ggcgggatcg
     2101 acaaacgaga gctggagaag ggtcccgcgg ccatcgatcg cgaggtcgcg cgccgcatgc
     2161 ccctggtgcg ggacggtggt ttcatcccca tgccggatca ctacatcacg ccgcagacct
     2221 ccctggccga cttccggtat tacctttccc gcatcggggg gatccggatc gatgcccccg
     2281 gcacccgtcc cgaggtgaca cgcgcatgaa gatggatttc cgcaagcacg attcgcacag
     2341 cgagcaagcc aacatcctgc ggatcgtgca ccgaagccgc cgcattaccc gacgccagat
     2401 cgtggacctc accggcttca gccaggccaa gatcagcatc gtcatcaacg agctgaagag
     2461 ccgaggtctg ctggcggagc tggaggagac ttcgagctcg ggagggcgca aagccaagct
     2521 cctgcagctc tcctcctcct ccagtcacgt catcggggtg gagatgggag ggtacgaggt
     2581 caaggcggcg ctgattgacc tggccggaaa cctcctgtgc tcccgcaaga tgccggcccc
     2641 atccgatatc accaacccag accaggtcct gaagatcatc gcggagttca tccaggattt
     2701 ccgagctacc gcctgtccgg cgggctccca tccgctgggg gtgggcatcg ctctttccgg
     2761 gatcacggat cgggcgacgg gagagtgcgt ctacttccgc aaccagcgcg cctgggaggg
     2821 gtaccaggtc ggcaggaaga tggaagagcg cctccacctt ccgtgcatcg tggatgactc
     2881 cagccggatg atggccgtgg ccgagtggct ctacggcgtg tgccgtgggg tcgacagctt
     2941 cgtgctcatc tccatcggca tgggaaccgg ctgcgggatc ttcgtggacg gggagatctt
     3001 tcggggcacc aacgggttcg gaggcgagct gggtcacatg gtgatcaagg agaacggccc
     3061 gcggtgcgtg tgcgggaact tcggctgcgt ggagagcttc atctccggct acgccctgga
     3121 gcggcggctt cgggaggcgc tgcaggacaa cgtctatact gccatgatgc acctggagga
     3181 tcgctcggca aaagagatcg taaaccacgc gctccagggg gacaagctgg cgtacagcat
     3241 cgtcacggac gccgcggagc acctggggat cgggatcgcg aacgtgatca acatcttcaa
     3301 tc
//
LOCUS       DASRZS010000469        10881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_252562, whole genome shotgun sequence.
ACCESSION   DASRZS010000469 DASRZS010000000
VERSION     DASRZS010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10881
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252562"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VFH83_11460"
     CDS             <1..625
                     /locus_tag="VFH83_11460"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET6487031.1"
                     /translation="LGYTGGLLFGDRDFIRTDSTVEGFLTLFDIPAFENWNLQFVLAA
                     HSELSFILPQYAFLNGSWGWQTITDQTDQVYIDGMNVGRGWGLMYGDALWDNKLELRM
                     PIIKEAIWLVGFFDAAGIWDEPWKMGPGDIATTFTTMNLQQFYFSVGAGIRFSIPQFP
                     IRLYLAWPFQINPANPGGWIVPRTGFSGINLGNVTLGFVISLGGNVF"
     gene            642..1196
                     /locus_tag="VFH83_11465"
     CDS             642..1196
                     /locus_tag="VFH83_11465"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET6487032.1"
                     /translation="MRRTALIGMLALATVLPLAAQQVTKIGICDFTRVLSTAYRQAKG
                     FRDFEAAQNDIKKEIASVTKAITDLQNQQLDADKAGNKTLSLKLQGQVNDQQTYLDNY
                     RKVKNAWLQQQYATLLSGPVLQDILDVVKFVAEAGGYSLIIRSDDVGGQLILYNVSEV
                     DITDDVIAEIFNRAGQKPPTTGSQ"
     gene            1197..1613
                     /gene="ruvX"
                     /locus_tag="VFH83_11470"
     CDS             1197..1613
                     /gene="ruvX"
                     /locus_tag="VFH83_11470"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET6487033.1"
                     /translation="MGRLLGIDLGARWVGLALTDPQGIIASPLETIPLESESSLVRRI
                     VDFSRKKGVDRVVIGLPLSADGSEGPGCARSRRIADRLSEEGVVCELHDESWSSREAE
                     EALRSTGRTRKAARSRVDSVAASLILQDYLASRPQS"
     gene            1622..4258
                     /gene="mutS"
                     /locus_tag="VFH83_11475"
     CDS             1622..4258
                     /gene="mutS"
                     /locus_tag="VFH83_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET6487034.1"
                     /translation="MAEPTPMMAQYRRIKERHTGSILFFRLGDFYEMFDADAREASAL
                     LDLTLTQRAGVPMCGIPYHAATSYIARLLAAGRKVAICEQTTPPGRGLMQRDVLEVIT
                     PGTVLDEGFLSRGANNYLVCIARAAETVALAYVDLSTGELAATSFSFEQRESVLRKEL
                     HRLDPREVITAESLLVEDPVIRDLLGQREGLLVNRYPEWSFDAETCRSRLERQLGVAN
                     LKGFGLSEQSTEVLAAGVLLDYVGETARHTLRHVSSLQIYGERSFVGLDEATQRNLEL
                     LTNLQDGSRKYTLLEVLDQTRTSPGARKLRRWILAPLLRREEIEERLTAVDALYRDQL
                     LLSKLRELLSASLDLERLTARLAMERAHAKDLLAIRATLQSALSMGPLLSGTAPPDDR
                     HPLRAVVAPLARNRDRMREAAELLDRAICDEPAITLTDGNLIRRGYDAELDRLHELAG
                     SARTILDAYLTEERAATGISSLKLRYNKVFGYSFEVSKANLPHVPPHFIRRQSLVGGE
                     RYTTDRLSDLESQINDASERIVEVERTLFLAVRARVAEAVPWLLEVSGAISELDVYQS
                     LAFAATLHGYARPTVVDAAELDIRDGRHPVVEAHLPGGAFVPNSLSCRGDEKFFVILT
                     GPNMAGKSTYLRQVALITLMAQIGSFVPAADARIGVADSIFCRVGATDNLARGESTFL
                     VEMNETAHILRSATPRSLVIMDEIGRGTSTRDGLAIAWAVCLYILEHVRCRTLFATHF
                     HELTALSHDAVSNLSMDVQEHAGEVVFLKRVREGPSSNSYGIHVARLAGLPAETLAYA
                     ERLMAEGIPQAAVLPTDAPPSPGPPSNAGRVARSQAGLFPVEELIAREILGTSLDRMT
                     PLEALNRIARWREELSHERDKA"
     gene            4287..4958
                     /locus_tag="VFH83_11480"
     CDS             4287..4958
                     /locus_tag="VFH83_11480"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET6487035.1"
                     /translation="MEGLLPGRCLLCGRWLLENSEPCSPVCPSCLEGLSPIGPRRCAS
                     CGMPLISERSQCVRCRSAGYSFSSNQALFPYAGAARELLRQLKFGGRRRVAAVFSDLI
                     ATAIAERAPGTWVVPVPPRAGRETPDGVELVCRALERRHDVGVLRALMRTGGAQQKTL
                     DLAQRRQNLKGRIHLRVPGQALPATVALLDDVFTTGATLDACARVLKESGCASVCSFT
                     LAIEE"
     gene            complement(4978..6204)
                     /locus_tag="VFH83_11485"
     CDS             complement(4978..6204)
                     /locus_tag="VFH83_11485"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET6487036.1"
                     /translation="MPGPVDPDPLDPYCSKTVGLKFSGVDLTFELSHGLFSSFAIDAG
                     TRLLLKTVAQRIDLDAIDGCLDLGCGVGVLGICVARRAPRSSVVLQDRDALAVAIAGR
                     NAKANACDGVSLDCGLGFWHLGDRVFDLILSNIPAKAGAPVLRHLLGTMANRLTPEGR
                     AAIVVVAPLAPFALETVNQAACVVDHQESTPSHTVLHFHRDVNQTPIPVAARSEDRAP
                     YVRATTRFSHGPISYTLETVYGLREFDTLDYATTLAFDLLGALHARGRILVWNPGQGH
                     MPVYLCSSQGNEIAEIHLAGVDFLELVATNRNLGAVDVMPSTSAALPCEAYLEGCCPE
                     GSMDLICASPHPVPRAGWQAGLSSAAARLLRPGGCLLVSGSSTEIQRYVQQQRGAQVI
                     GSRKLYGHRAVLLRKL"
     gene            6383..6850
                     /locus_tag="VFH83_11490"
     CDS             6383..6850
                     /locus_tag="VFH83_11490"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487037.1"
                     /translation="MRETIQPLLDGLGLFLVELAVGRLRGSPRVSCVIYRPGGVGIND
                     CARASELILPRLQTVEGLADVSLEVSSPGIERTFHSPSEYGIFTGRGVRVLAGDATEW
                     LPGVIVKTDGTTLWLRRGRRTMELAISSIRRARLDYAAEIEENKRTEEADHAI"
     gene            6840..8402
                     /gene="nusA"
                     /locus_tag="VFH83_11495"
     CDS             6840..8402
                     /gene="nusA"
                     /locus_tag="VFH83_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET6487038.1"
                     /translation="MQSDLSQAIKQLVQERGIPEDLVMSTIENLLLAAYKRRFGVVDN
                     AVVRFSEDGTEVTLFAKKKIVVDDDLDDPVYELPLSEALELNEECEVGDELLIEINPR
                     EFDRVSVQSAKQKARQSLREIQKDTLYSEFKDKVGEMIIGYYQRERNGNIYVDLGKTE
                     GILPKKYQSPREIYHPNDRIKALIYEVVKTPTGLQIVLSRNHAEFVKKIFELEVPEIY
                     DKTIEIMKIVREPGYRTKIAVRSTREDVDPVGACVGLKGVRIQAVVRELEGEKIDILR
                     YSLDSRSFIKNALSPAEVNQVVILDEAKKLALAIVPDAQFSLAIGKQGLNVRLANRLT
                     DWNIDVKTETQFSEMDISAEAKRAVSALFGEPEEEIQKISELPGIPARLVEILEKNKI
                     TDIEALVGLSPEQLAALEGITADDIKTLHQIIEENVEIVEEEPERFAPEPEAEAETAE
                     PAAAAQEAPQAEAAAPTAAEAGPGSAEAEAEVEEEETYECPECGAVITPDMATCPNCG
                     VGLTFEDGPEQE"
     gene            8416..>10881
                     /gene="infB"
                     /locus_tag="VFH83_11500"
     CDS             8416..>10881
                     /gene="infB"
                     /locus_tag="VFH83_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET6487039.1"
                     /translation="MPEELDSKKKPKTTLIKHRKIEEEQPPAPLAAPPAEKKKVVVVK
                     KKVVPVKKIVHRAPVVEQPAPAPAAAAPIPAATAHPPSPAPETREAAPTPAPTPAAAA
                     QPPAAAPVAAAPQQAAPPRPAPTRTAPADQPRPQPAAPVPPAAAAASAATRPALRPAH
                     PAGPGMHPSPVTASQSVVPPGAPAAQAPTDRPRFVTQNRGPEPRPSVRESVREKMRLT
                     PLPPRPAGAPPRPGFRGPGSGGPGYPPRAGGRPPMRPGGGGGAPSSGGTDDKKPPAKK
                     FFTKGKKKIEYPKRKHDQPEEKYLQVKKKTVIMANPVPKEISITEGITVSELARKMNL
                     KGSDLIHKLMGMGMMVTINQQIDSDTAQLLAAEYDCKVNVVSLYDETVIEEEKEVEGD
                     LRPRPPIVTIMGHVDHGKTKLLDAIRSTNVAEGEYGGITQHIGAYSVDLPTAAGGLKK
                     IVFLDTPGHEAFTMMRARGAQVTDIVVLVVSAVDGVMPQTIEAIAHAKQAEVPIIVAV
                     NKVDLAEANVELVKRQLSEQNLTPEEWGGTTIYNEVSALKKTGIKELLESILLQAEVL
                     ELKASYATRASGIVLESRVDMGRGIVGTVILQKGTLRSGDSFVAGIYSGKVRAVFNDR
                     GERVSEATPSLPVEVIGFTSIPNAGDPFQMTDSEQTAKLIGSKRQELKKLEESKSVKK
                     VTLDNLYEQIREGQVQELKVIIKADVAGSVEALKTSLQRLSTSEIKLDVIQASAGPIN
                     EGDVMFASTSNALIVGFHVRPSAAAQNLADREKVEIRKYNIIYDALEDIRSAMEGLLK
                     PELREEQIGTVEVRNTFKIPKIGT"
BASE COUNT         2036 a         3473 c         3649 g         1723 t
ORIGIN      
        1 cctcggctac accggcgggc ttcttttcgg ggaccgggac ttcattcgga cggactcgac
       61 cgtggaaggc ttcctcaccc tcttcgacat accggccttc gaaaactgga acctgcagtt
      121 cgtcctggcg gcgcactcgg agctctcgtt cattctcccg cagtacgcgt tcctgaacgg
      181 tagctggggg tggcagacca tcacggacca gacggaccag gtctacatcg atggaatgaa
      241 cgtcggccga ggctggggcc tcatgtatgg agacgcgttg tgggacaaca agctcgagct
      301 tcggatgccc atcatcaagg aagccatctg gctggttgga ttcttcgacg ccgcggggat
      361 ctgggacgag ccctggaaga tgggtcccgg ggacatcgcg acaaccttca cgaccatgaa
      421 cctgcagcag ttctacttct cggtcggggc cgggatccgg ttcagcatcc ctcagttccc
      481 catccgtctc tacctggcgt ggccgttcca gatcaacccg gcgaacccgg ggggatggat
      541 cgtgccccgg acaggtttct ccgggatcaa cctggggaac gtcacgctcg ggttcgtcat
      601 ctctctgggc ggcaacgtgt tctagagggg agggagtcac catgagacga accgcgctga
      661 tcgggatgct ggcgctcgca accgtcctgc cgctggccgc gcagcaggtg accaagatcg
      721 gcatttgtga cttcacccga gttctcagca ctgcctaccg ccaggcaaag gggtttcggg
      781 atttcgaagc cgcgcagaac gacatcaaga aggagatcgc ctcggtcacc aaggccatta
      841 cggacctcca gaaccagcag ctcgacgcgg acaaggccgg caacaagacc ctctccttga
      901 agctccaggg ccaggtcaac gaccagcaga cctacctgga caactaccgc aaggtgaaga
      961 acgcatggct ccagcagcag tacgcgaccc tgctttccgg tcccgtgctc caggacatcc
     1021 tggacgtggt gaagttcgtg gccgaggccg gtggctactc cctgatcatc cgcagcgacg
     1081 acgtcggcgg ccagctcatc ctgtacaacg tgagcgaggt ggacatcacc gatgacgtga
     1141 tcgcggagat cttcaaccga gccggtcaga agccgcccac caccgggagc cagtagttgg
     1201 ggagactcct cgggatcgat cttggcgccc gatgggtcgg gctggccctc acggacccgc
     1261 aggggatcat cgcctcgccg ctggaaacca tcccgctgga gtcggaatcc agtctggtcc
     1321 gcaggatcgt ggacttctca cggaagaagg gtgttgatcg ggtggtcatc gggcttccgc
     1381 tgagcgcgga cgggagtgaa gggcccggct gcgcccgctc gcgacgcatc gcggaccgcc
     1441 tttccgaaga gggcgttgtc tgcgagctgc acgacgagag ctggtccagc cgggaggccg
     1501 aagaggccct gcgctccacg ggacgcaccc ggaaagccgc aaggtcgcgc gtggactccg
     1561 ttgcggcgtc gttgatcctt caggattacc tggcctcccg tccgcagagc tgagtgcggc
     1621 catggctgag ccgaccccca tgatggccca gtaccggcgg atcaaggagc ggcacaccgg
     1681 atccatcctc ttcttccggc tcggggactt ctacgagatg ttcgacgcgg acgcccggga
     1741 agcctccgca ctcctcgatc tgaccctcac ccagcgggcc ggggtcccga tgtgcgggat
     1801 tccctatcac gcggccacat cctatatcgc ccggctcctg gcggccggac gcaaagtcgc
     1861 catctgcgag cagaccacgc cgcccggtcg ggggctcatg cagcgtgacg tcctggaggt
     1921 aataacaccg ggcacggtgc tcgacgaggg gttcctgtct cgaggcgcca acaactacct
     1981 ggtgtgcatc gctcgggcgg cggagacggt tgccctggcc tacgtggacc tgtccacggg
     2041 agagctggcc gccacctcgt tctctttcga gcagcgggaa tccgtgctga gaaaggaact
     2101 gcaccgtctg gaccctcgag aggtcatcac cgccgaatcg ctcctggtgg aagatccggt
     2161 gatccgggat ctcctcggac aacgggaagg cctgctggtg aaccggtatc ccgagtggag
     2221 cttcgacgct gagacctgca ggtcccgact ggaacgccag ctcggggtgg ccaacctcaa
     2281 aggcttcggt ctctcggagc aatcgacgga ggtgctcgcc gccggtgtgc ttctggacta
     2341 cgttggggag accgcccgac acaccttgcg acacgtctcc tctctccaga tatacgggga
     2401 acggagcttc gtggggctgg acgaggccac gcagcggaac ctcgagctgc tgaccaacct
     2461 gcaggatggc agccgcaagt acaccctcct ggaggtgctg gaccagacgc gcacctctcc
     2521 cggcgcccgc aagcttcggc gctggatcct ggcgcccctg ctgcggcggg aggagatcga
     2581 ggagcgtctg accgccgtgg acgcgctcta tcgggaccag ctgctcctgt ccaagctcag
     2641 agagctgcta tcggcatccc tcgacctgga gcgtctgacg gcgcgcctgg caatggagcg
     2701 agcgcacgca aaggaccttc ttgccatccg ggcaacgctt cagtccgccc tttccatggg
     2761 accgttgctg tccggaacgg ctccaccgga tgacaggcac ccgcttcgcg ccgtcgtcgc
     2821 accgctggca aggaaccggg accgtatgcg ggaagcggcg gagcttctgg accgggcgat
     2881 ctgtgacgag cccgccatca cgctgacgga cggcaacctg atccgtcggg gctacgacgc
     2941 ggagctggac cggctgcacg agctcgcggg aagcgctcgc accatcctgg atgcgtatct
     3001 cacggaggag cgcgccgcaa ccgggatatc ctccctcaag ctgcgctaca acaaggtctt
     3061 tggctactcc tttgaggtga gcaaggccaa cctccctcac gtcccgccgc atttcatccg
     3121 gcggcagtcg ctcgtaggag gggagcgcta caccacggac cgtctgtcgg acctggagtc
     3181 tcagatcaac gatgcctctg aacggatcgt ggaggtcgag cgtaccctct tcctggcggt
     3241 gcgcgctcgg gtggccgagg cggtgccctg gcttctggag gtctccgggg cgatatcgga
     3301 gctggacgta taccagtcac tggccttcgc cgcgaccctg catgggtacg cccgtccgac
     3361 cgtcgtggat gccgcggagc tcgacattcg agatgggcgg cacccggtgg tcgaggcgca
     3421 cctaccgggc ggggcattcg tgccgaacag cctgagctgc cgcggagacg agaagttctt
     3481 cgtgatcctg accggtccga acatggcagg gaagtcgacc tacctgcgcc aggtcgcgct
     3541 catcaccctc atggcacaga tcgggtcttt cgtaccggcg gcggacgcca ggataggggt
     3601 cgcggactcc atcttctgcc gcgtcggcgc aacggacaac ctggctcgcg gcgagtccac
     3661 gttcttggtg gagatgaacg agacggccca catcctgcgc tccgccaccc cccggagtct
     3721 cgtgatcatg gacgagatcg gccgaggaac cagcacgcgg gatgggctgg ccatcgcctg
     3781 ggcggtctgc ctgtatatcc tcgagcacgt gcgctgccgg acgctcttcg ccacgcactt
     3841 ccacgagctc accgctctct cccatgatgc ggtctccaac ctctccatgg acgtccaaga
     3901 gcatgccggg gaggtcgtct tcctgaagcg ggtgcgggag ggtccctcaa gcaactctta
     3961 tggcattcac gtcgcgcgtc tggccgggtt gccggcggag accctcgcgt atgccgagcg
     4021 ccttatggcg gaggggatcc cccaggcagc cgttctgccg accgacgcgc cgccgtctcc
     4081 tgggccaccg tccaatgccg gccgagtggc gcgcagccag gccgggctgt ttcccgtgga
     4141 ggagctgatc gcccgggaga tcctcgggac gtcgctggac cgtatgacgc ctctggaagc
     4201 tctgaaccgc atcgcccgat ggagggagga gctctcccac gagcgggaca aggcctgatc
     4261 gggtcggccg gatcgcctgg agggtggtgg aggggctcct tcccggccgc tgcctgcttt
     4321 gcggccgttg gctcctggaa aacagcgagc cgtgcagccc ggtatgcccc agctgcctcg
     4381 agggtctgag ccccataggc cctcggcggt gtgccagctg cgggatgccc cttatctcgg
     4441 agcgttccca gtgcgtacgc tgccggtcgg ccggctactc cttctcgagc aaccaggcgc
     4501 tcttccccta cgcgggagcg gcgcgagagt tgctgcggca gctgaagttc ggaggacgga
     4561 ggagggtggc cgcggttttc tccgatctga ttgccacggc catcgccgaa cgggcgcccg
     4621 gcacctgggt ggttcccgtc ccacccaggg cgggacggga gacaccggac ggcgtggagc
     4681 tggtctgtcg cgctttggag cgccgccacg acgtcggcgt tctgcgcgct ctcatgcgga
     4741 ccggcggagc ccagcagaag accctggatc tggcgcagcg ccggcagaac ctgaagggga
     4801 gaatccatct gcgggttccg gggcaagccc tgccggccac ggtggcgctg ctcgatgacg
     4861 tcttcacgac cggcgcgacc ctggacgcgt gcgcccgcgt cctgaaggaa tcaggctgcg
     4921 cctccgtgtg ctccttcacc ctggccatcg aggagtaggg caggccgccg ggaagggtca
     4981 gagcttccgc agcagcacgg ccctatgccc gtacagcttt cggctcccga tcacctgggc
     5041 gcccctctgc tgctgtacgt agcgttggat ctcggtgctg ctgccggaaa ccaggaggca
     5101 accgccgggt cgaaggagcc gggcggcggc ggatgagagc cccgcctgcc accccgcgcg
     5161 gggaaccggg tggggcgagg cgcagatcag atccatgctt ccctcgggac agcatccctc
     5221 caggtacgcc tcgcagggca gcgcggctga agtggaaggc atcacgtcaa cggctcccag
     5281 attgcggttc gttgccacga gctccagaaa atccactcca gcaaggtgga tctcggcgat
     5341 ctcgttcccc tggctggaac agaggtatac cggcatgtgc ccctggcccg gattccagac
     5401 caggatgcgg ccgcgcgcgt ggagggcacc cagcaggtcg aaggccagcg tcgtggcgta
     5461 atcgagcgta tcgaactccc gcaggccgta caccgtctcc agggtgtagg agatggggcc
     5521 gtgggagaat cgggtggtcg cgcggacata aggagcacga tcttccgaac gggcggccac
     5581 cgggataggc gtctgattca cgtcccggtg gaagtgcagg accgtgtgcg agggcgtact
     5641 ctcctggtgg tccacgacgc acgccgcctg gttcacggtc tccagggcga agggagccag
     5701 cggggccacc accacgatcg ccgcgcggcc ctctggcgtg agccggtttg ccattgtccc
     5761 caggaggtga cgcagcacgg gcgcgcccgc cttggcgggg atgttcgaga ggatcaagtc
     5821 aaagacgcgg tcccccaggt gccagaatcc gagcccgcag tccagcgaga cgccgtcgca
     5881 tgcgttcgcc ttggcgttcc gtcccgcgat ggcaacggcg agcgcatcgc gatcctggag
     5941 caccacggag gaacgaggcg cccgacgggc cacgcagatc cccagcacgc ccacgccgca
     6001 ccctagatcc aggcatccgt cgattgcgtc gagatcgatc cgctgggcga cggtcttcaa
     6061 gagcagacgg gtgccggcgt cgatggcgaa gctgctgaac agcccgtggg acagctcgaa
     6121 cgtcaggtcg acgccgctga acttgaggcc cacggtcttg ctgcagtacg ggtccagggg
     6181 atcgggatcg acaggtccag gcacgcgcca gtatagggga gcttgcggcc ggattgacaa
     6241 gggggtcgtt tttggagtat gtttcgccga gaaagagtcg ggcgggactc ttttttttgt
     6301 acccgggggg tggtttcgag cgggcacggc tgccgaagat ctgaggcgag agggtttgta
     6361 ccaggagcac ttcaccagca cggtgagaga gacgattcaa cctttgctcg acggactggg
     6421 cttgttcctc gtggagttgg cggtgggccg gctcagaggg agcccgcggg tgagctgcgt
     6481 gatctatcgc cccgggggcg tcggcatcaa cgactgcgcg cgggccagcg agctgatcct
     6541 cccgcggctg cagaccgtgg agggcctggc cgacgtcagc ctggaggtaa gctcgcccgg
     6601 aatcgagcgc acgtttcaca gcccctccga gtacgggatt ttcacaggaa ggggtgtgcg
     6661 cgtccttgcg ggggatgcca cggagtggct ccccggcgtg atcgtgaaga ccgacgggac
     6721 cacgctctgg ctgcggcgag gtcgccggac gatggagctc gcgatctcct ccattcggag
     6781 ggcacgtctg gactacgcgg ccgagattga agaaaacaag agaaccgagg aagccgacca
     6841 tgcaatctga tctgtcgcag gccatcaagc agctcgtcca ggagcgggga atccccgagg
     6901 acctggtgat gagcacgatc gagaatctgc tcctggccgc ctacaagagg cggttcgggg
     6961 tcgtggacaa cgccgtggtg cgcttcagcg aggacggcac ggaggtgacg ctcttcgcga
     7021 agaagaagat cgtcgtcgac gacgacctcg acgatccggt ctacgagctt ccgctctccg
     7081 aggcgctcga gctcaacgag gaatgcgagg tcggggacga gctcctcatc gagatcaacc
     7141 cgcgggagtt cgatcgcgtc tcggtccaga gcgccaagca gaaagcccga cagagcctgc
     7201 gggagatcca gaaggatacc ctgtactcgg agttcaagga caaagtcggg gagatgatca
     7261 tcgggtacta ccagcgggaa cgcaacggca acatctacgt ggaccttgga aagaccgagg
     7321 gaatcctgcc gaagaagtac cagtccccgc gggagatcta ccatcccaac gaccggatca
     7381 aggcgctgat ctacgaggtc gtgaagacgc ccaccggtct gcagatcgtg ctctcgcgca
     7441 accacgcgga gttcgtgaag aagatcttcg agcttgaggt ccccgagatc tacgacaaga
     7501 cgatcgagat catgaagatc gtccgggagc cggggtatcg gaccaagatc gcggtgcgat
     7561 ccacccggga ggacgtggat ccggttgggg cttgcgtggg actgaaaggc gttcgcatcc
     7621 aggccgttgt ccgagagctg gaaggcgaga agatcgacat cctccgctac tccctggatt
     7681 cgcgcagctt catcaagaac gcgctgtcac cggcggaggt gaaccaggtt gtgatcctgg
     7741 acgaggccaa gaagctggcc ctggccatcg tgccggacgc gcagttctcc ctggccatcg
     7801 gaaagcaggg gctcaacgtc cgcctggcca atcgcctgac ggactggaat atcgacgtaa
     7861 agacggaaac ccagttctcg gaaatggaca tctccgcaga ggccaagcgg gcggtatcgg
     7921 cgctcttcgg agagcccgag gaagagatcc agaagatctc cgagcttccg ggcatccccg
     7981 cgcgtctggt ggagatcctc gagaagaaca agatcaccga catcgaggcc ctggtcgggc
     8041 tcagccccga gcagctcgcc gcgctggagg ggatcaccgc ggatgacatc aagacgctcc
     8101 accagatcat cgaggagaac gtcgagatcg tggaggagga acccgagcgg ttcgctccgg
     8161 agccggaggc cgaggcggag acggccgagc cggcagccgc ggcgcaggaa gcgccgcagg
     8221 ccgaggcggc ggccccgacg gcggcggagg ccggccctgg gagcgcggag gcggaagccg
     8281 aagtggaaga ggaagagacc tacgagtgcc cggagtgcgg cgccgtgata acgccggata
     8341 tggccacttg tccaaactgc ggcgtgggtc taacctttga ggacggtccc gagcaggaat
     8401 aagagaagag caggtatgcc tgaggaattg gacagcaaga agaagcccaa gacgaccctg
     8461 atcaagcacc gcaagatcga ggaggagcag ccgccggcac ccctggccgc gcctcccgcg
     8521 gagaagaaga aggtggtcgt cgtcaagaag aaggtggtgc cggtcaaaaa gatcgtccac
     8581 cgggccccgg tggtagagca gccggccccg gcgcccgccg ctgcggcgcc catcccggcc
     8641 gcgacggcgc atccgccgtc cccggctccg gagacccgtg aagcggctcc gactccggcg
     8701 cccacgccgg ccgccgcagc gcagccaccg gcggctgctc cggttgccgc ggcgcctcag
     8761 caggcagccc caccgcgtcc cgctccgacc cgcacggcgc cggcggacca gcccaggcct
     8821 cagccagcgg caccggtccc cccggccgcg gctgccgcct cggcggccac ccgaccggcc
     8881 ctgcgtccgg cgcatcccgc gggacccggc atgcatccgt cccccgtgac cgcgagccag
     8941 tcggtcgtcc cgcccggggc accggccgcg caggcgccca cggaccggcc gcgattcgtg
     9001 actcagaatc gaggaccgga gccccgtccc tccgtacggg agtccgtacg ggagaagatg
     9061 cggcttactc cgcttccgcc cagaccggct ggggcgcctc cccgaccggg gttccgcgga
     9121 cccggctctg gcggccccgg gtatcctcct cgcgccggcg gtcggccgcc aatgcggcca
     9181 gggggaggcg gaggagcgcc aagctcgggc ggcacggatg acaagaagcc gccagccaag
     9241 aagttcttca ccaagggcaa gaagaagatc gagtatccca agaggaagca cgatcagccc
     9301 gaggagaagt acctccaggt caagaagaag accgtgatca tggccaaccc ggtgcccaag
     9361 gagatctcga tcacggaggg catcacggtc tccgagcttg cccgcaagat gaacctcaaa
     9421 ggctcggatc tgatccacaa gctcatgggc atgggcatga tggtcacgat caaccagcag
     9481 atcgactcgg atacggccca gcttctggcg gcggagtacg actgcaaggt caacgtggtc
     9541 tcactgtacg acgagacggt gatcgaggag gagaaggagg ttgagggaga cctccgcccc
     9601 cggcccccca tcgttacgat catgggtcac gtagaccacg gcaagacgaa gctcctggac
     9661 gccatccgaa gcaccaacgt tgcggaaggg gaatacggcg gaatcaccca gcatatcggc
     9721 gcctattccg tcgacctccc cacggcggcc ggcggcctca agaagatcgt gttcctggat
     9781 accccgggcc acgaggcgtt cacgatgatg cgggcgcgcg gcgcccaggt cacggacatc
     9841 gtggtcctgg tggtttctgc cgtggacggc gtgatgccgc agaccatcga agccattgct
     9901 cacgcgaagc aggccgaagt tccgatcatc gtggccgtca acaaggtgga tcttgcggag
     9961 gccaatgtcg agctggtgaa acggcagctc tcggagcaga acctgacgcc cgaagagtgg
    10021 ggcgggacca cgatctacaa cgaggtgtcc gccctgaaga agaccgggat caaggagctc
    10081 ctggagagca tcctgcttca ggccgaggtc ctggagctga aggccagcta cgccacccgc
    10141 gcctccggta tcgtgctgga gtcaagggtg gacatgggcc gaggcatcgt tggcaccgtg
    10201 atacttcaga agggcaccct ccggtccggg gactccttcg tcgccggcat ctactcgggg
    10261 aaggttcgcg ccgtcttcaa cgatcgcggg gagcgcgtga gcgaggccac gccgtctctg
    10321 ccggtcgagg tgatcgggtt caccagcatc ccgaatgcgg gggatccgtt ccagatgacg
    10381 gactccgagc agaccgccaa gctgatcgga agcaagcgcc aggagctcaa gaagctggag
    10441 gaatcgaaga gcgtcaagaa agtgaccctg gacaacctct acgagcagat ccgggagggc
    10501 caggtacaag agctcaaggt catcatcaag gcggatgtgg ccggctccgt agaggcgctc
    10561 aagacctcgc ttcagaggct gtcgacgtcg gagatcaagc tggacgtcat ccaggcgtcc
    10621 gccgggccca tcaacgaagg ggacgtcatg ttcgcgtcca cctcaaacgc tctgatcgtc
    10681 ggcttccacg tgcgccccag cgccgccgcg cagaaccttg cggatcgtga gaaggtggag
    10741 atccgcaagt acaacatcat ctacgatgct ctggaggaca tccgttccgc catggaaggt
    10801 ctgctcaaac ccgagttgag ggaggagcag atcggcacgg tggaggtgcg caacacgttc
    10861 aagattccca agatcgggac g
//
LOCUS       DASRZS010000470         5908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3329354, whole genome shotgun sequence.
ACCESSION   DASRZS010000470 DASRZS010000000
VERSION     DASRZS010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3329354"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VFH83_11505"
     CDS             complement(<1..970)
                     /locus_tag="VFH83_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487040.1"
                     /translation="MYYRYVIRRVLNAVAIYVVIVLAFSLMFNTVNETNARAEIEESI
                     RAESMRLKNMNARQIQQWMTERRAFKYHAYHLDQPFVGRVVWHTIDTVTFQYGLSNGM
                     KSSRGDREVWKIVVEVLPRTILLFTTAIVFEVGFGIWLGLKMAQRAGRLLDKSSSVMT
                     MIVNGMPTWWLGMILIMVFAYKVPLFPSGGMHTLPPPQGVMRLVDVLWHMALPLVTLL
                     LTGFWGVALLARNIVLSILQEDYIMAARARGLPERTVLFGHTLRTAAPPFTTIALLSL
                     LTSVFGGIVFEGIFSWPGMGNLYWISIESNDIPVLLGLLSVTTGLYI"
     gene            complement(1017..3545)
                     /locus_tag="VFH83_11510"
     CDS             complement(1017..3545)
                     /locus_tag="VFH83_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6487041.1"
                     /translation="MKRLLLIALLIAAGTSLLVAQAAKKGPTLDKVIFDVRMQEDIGL
                     QDTAEGKADLFLYGVQGNTYKDLPQATKDKLDVFSSPSGLWSIELNPYPNKAPYIATV
                     DKKDSFNPLAMQEIRFALNDLINRKYIVDEITGGAGGACFTSATPGQPGTYQYDLVAS
                     KLGMTVTGNEKKAITDITNALTRASELPDLKGRLVKGSPWWTFDGDPVTINFVIRVDD
                     PNGRLKEGHYIADQIEKTGLKVNRMELSRAKALDLAYNSDPAKFEWSMYTEGWGAGST
                     NAYWEVNIAQWYAPFYGYMPGGGNADFWNYTQPEIDQLMQKITTGNFSGEKEYWDSIL
                     RATELGLKDSVRINVSYQSQLYTANKARFIGRMVYGLGDGLGTNWSLVTADVKPDANG
                     QKVLRALQFSAKGALFMGAWDPVGTDGMADTYTLNVSKPCSDEATFQAPNSAMVTPWR
                     ATWKDLQTKVTPGKDDKGNPILVGQIPVPPDAVLYNSKTHTWDKVGDGVVAFSKATYT
                     YKLGNWHDGTPMRMADLMYAFAFITDWATKDGDNDKYFDSAYRDYWKPTLDTTKGIVL
                     NPDGTVTTYFNYNHMDQGLVAASGALNWRVSAAGQPNIVSWTILEAISDLAAEGGPSK
                     TAWSFSADPSLVEVDLLTPNCLADIRAKLTDFIQTQHVPDSVKDWITPDQAVAAYQNS
                     IAFIDAHKNAYVSNGSFYINAVDLSANYIELAANRDPTYPFVPGYWTKLFTTTTTRID
                     AVQPPAVATHGKDVKVTVKVSAVQYPLNTARAADTTAKVTLTLVTPTGDKEYKGVFSR
                     AGQFVVTIPAADTKSLKAGSYTIVVQSLFKTEAPSVQPASIVFF"
     gene            3728..4402
                     /locus_tag="VFH83_11515"
     CDS             3728..4402
                     /locus_tag="VFH83_11515"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HET6487042.1"
                     /translation="MALSSVTRSVPGLTGVSLRGFGDLTIEQKPEAEGRETIAIEADP
                     TILDRVLTEVRGGTLVIGFSMPWYEWLTWWLQWIFVADKRIRFRLTAARIESVHISGA
                     GRVTVPSLRCNQLDVGISGAGKIVVSDIAATEVQTRVSGAGNIELTGAASRLEARISG
                     AGNIRAEDLSTRRTIASISGSGRLTANATEELDAHISGAGGVGYRGNPKVIQRVSGAG
                     KVWAIR"
     gene            4741..>5908
                     /gene="aspS"
                     /locus_tag="VFH83_11520"
     CDS             4741..>5908
                     /gene="aspS"
                     /locus_tag="VFH83_11520"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET6487043.1"
                     /translation="METMKRTVTCGDLRASDAAKSVTLNGWVHRHRDHGGIRFLDIRD
                     RYGVTQVVVDADAAEDLRSVAAELKFEYCVAVEGTVRQRPASMANPGMATGEIEVKAS
                     RIVILSRCDVLPFMVDERADAREDTRLRYRYLDLRTGAMQRNIALRHRAAFATREYLT
                     QRGFFEIETPMFIKSTPEGARDYLVPSRLNPGKFYALPQSPQLYKQLLMVSGFDRYFQ
                     IARCFRDEDARGDRQPEFTQIDIEMSFVSRDDVFALVEGLMAHIVKQSIGKDLATPFQ
                     RLPYDEAMSLYGSDKPDLRFDLAMKDFGPFALKGGFEVFRKVAEGGGVVKALVAPGCG
                     EYSRKQITDLEAVAKVFGAAGLAWMKVTAAGLEGGVSKFYAEQAAQIPAALGAKA"
BASE COUNT         1002 a         1815 c         2041 g         1050 t
ORIGIN      
        1 cgatgtagag ccccgtggtc accgagagaa gtcccagcag cacggggatg tcgttggact
       61 cgatggagat ccagtagagg ttccccatcc ccggccaact gaagatcccc tcgaacacga
      121 tcccgccgaa caccgaggtc aggagggaga gcagcgcgat ggtcgtaaaa gggggggccg
      181 cggtgcgcag ggtgtgcccg aagaggacgg tccgctccgg gagaccgcga gcgcgggccg
      241 ccatgatgta gtcctcctgc aggatgctca gcacgatgtt gcgcgccagg agggccacgc
      301 cccagaaacc ggtgagcagg agcgtcacca gcggcagcgc catgtgccac aggacgtcca
      361 ccagccgcat caccccctgg ggcggcggca gggtatgcat cccgccggag gggaagagcg
      421 ggaccttgta ggcgaacacc atgatcagga tcatgccgag ccaccaggtg ggcatcccgt
      481 tgacgatcat cgtcatcacg gaggaggact tgtccaggag acgccctgcc cgttgcgcca
      541 tcttcagacc cagccagatg ccgaacccta cctcgaacac gatggcggtg gtgaacagaa
      601 ggatggtccg cgggaggacc tcgacgacga tcttccagac ctcccggtcg ccacgggagc
      661 tcttcatccc gttggagagg ccgtactgga aggtgacggt gtcgatcgtg tgccagacga
      721 cgcggcccac gaacggctgg tccagatggt acgcgtgata cttgaacgcg cgccgctccg
      781 tcatccactg ctggatctgc cgggcgttca tgttcttcag gcgcatgctc tccgcgcgga
      841 tggactcctc gatctccgcg cgcgcgttgg tctcgttgac cgtgttgaac atgagggaga
      901 acgccaggac gatcacgaca tagatcgcta cggcgttgag gacccggcgg atgacatagc
      961 ggtaatacat cggagacggg ggcggctccg aagggagccg cccagggaat ccagtcttag
     1021 aagaacacga tggaggccgg ctgaacggaa ggggcctccg tcttgaagag ggactgcacc
     1081 acgatggtgt agctgccggc cttgaggctc ttcgtgtccg ccgccgggat ggtcaccacg
     1141 aactgaccgg cccgggagaa cacgcccttg tactccttgt ctccggtggg ggtcaccagg
     1201 gtgagggtga cctttgccgt ggtgtccgcc gccctcgccg tgttcagcgg atactggacc
     1261 gcggagacct tgaccgtgac cttcacgtcc ttgccgtgcg tggcgaccgc gggaggctgc
     1321 accgcgtcga tccgcgtggt cgtggtggtg aagagcttgg tccagtagcc cggcacgaac
     1381 gggtaggtgg ggtcccggtt ggccgcgagc tcgatgtagt tggccgagag atccaccgcg
     1441 ttgatgtaga aggatccgtt ggagacgtag gcgttcttgt gggcgtcgat gaaggcgatc
     1501 gagttctggt acgcggccac tgcctggtcc ggggtgatcc agtctttcac ggaatccggt
     1561 acgtgctgcg tctggatgaa gtccgtgagc ttggcccgga tgtccgccag gcagttgggg
     1621 gtcaggagat ccacctccac cagcgagggg tctgccgaga agctccaggc ggtcttgctg
     1681 gggccgcctt cggccgccag gtcggaaatg gcctccagga tggtccagga gacgatgttg
     1741 ggctgaccgg ccgcggagac cctccagttc agcgcgccgc tggccgccac caggccctgg
     1801 tccatgtggt tgtagttgaa gtaggtggtg accgtgccgt cgggattcag gacgatgccc
     1861 ttggtggtgt ccagggtggg cttccagtag tcccggtagg cggaatcaaa gtacttgtcg
     1921 ttgtcgccgt ccttggtggc ccaatccgtg atgaaggcga aggcgtacat caggtccgcc
     1981 atccgcatgg gcgtgccgtc gtgccagttg cccagcttgt aggtgtaggt ggccttgctg
     2041 aacgccacca cgccgtctcc caccttgtcc caggtgtggg tcttggagtt gtagaggacc
     2101 gcatccggcg gcaccgggat ctgacccacc aggatggggt tgcccttgtc gtccttcccc
     2161 ggcgtcacct tggtctgcag gtccttccag gtggcgcgcc acggggtgac catggcggag
     2221 ttgggcgcct ggaacgtagc ctcatccgag cacggcttcg agacgttcaa cgtgtacgtg
     2281 tcggccattc cgtccgttcc caccgggtcc caggcgccca tgaagagagc gcccttggcg
     2341 gagaactgaa gggcccggag gaccttctgg ccgttcgcgt ccggcttcac gtccgccgtg
     2401 accagggacc agttggtgcc aagaccgtcc cctagcccgt acaccatgcg gccgatgaag
     2461 cgcgccttgt tcgcggtgta gagctgggac tggtaggaga cgttgatgcg gacggagtcc
     2521 ttcagaccga gctccgtggc gcgcaggatg gagtcccagt actccttctc gccgctgaag
     2581 ttgccggtgg tgatcttctg cataagctgg tcgatctcag gctgagtgta gttccagaag
     2641 tcggcgtttc caccgccggg catgtagccg tagaacgggg cgtaccactg ggcgatgttc
     2701 acctcccagt aagcgttcgt ggagccggcc ccccagccct cggtatacat gctccattcg
     2761 aacttggcgg ggtcgctgtt gtaggccagg tccagagcct tggcccggct cagctccatg
     2821 cggttgacct tgaggccggt cttctcgatc tgatccgcga tgtagtggcc ctccttcaag
     2881 cggccgttgg gatcgtccac gcggatgacg aagttgatgg tgacggggtc accgtcgaag
     2941 gtccaccacg gggaaccttt cacgaggcgg cccttgagat ccggcagctc ggaggcccgg
     3001 gtgagagcgt tggtgatgtc cgtgatggct ttcttctcgt tccccgtcac ggtcattccg
     3061 agcttgctgg ccaccaggtc gtactggtag gtgccgggct gtcccggagt ggcggaggtg
     3121 aagcacgcgc caccggcgcc gccggtgatc tcgtccacga tgtacttgcg gttgatcagg
     3181 tcgttcaggg cgaagcggat ctcctgcatg gccagcggat tgaaggagtc cttcttgtcc
     3241 acggtggcga tgtagggcgc cttgttggga taggggttca gctcgatgga ccagaggccc
     3301 gacggggagc tgaacacgtc cagcttgtcc ttggttgcct ggggcaggtc cttgtaggtg
     3361 ttgccctgca cgccgtaaag gaaaagatcc gccttgcctt ccgcggtgtc ctggagaccg
     3421 atgtcttcct gcatgcgaac gtcgaagatg accttgtcga gggtgggccc cttcttggct
     3481 gcctgcgcaa cgaggagcga ggtcccggcc gcaatgagaa gagcgatcag cagaagcctt
     3541 ttcattggtt ctctccttcc aagggactgg aatgggcggt gccagccgcg ccgacgcacc
     3601 ggggtagcgg cacgaggcca gtagtaccat agaagagtag cgggcgcaac gtcaggagca
     3661 gacggtgcct tgcgcggtgt tgtaccattc ggcagcaacg ttccgaggga gaagaaaggg
     3721 ggcatccatg gcgctttcaa gcgttacgcg aagcgtgccg gggctcaccg gcgtcagcct
     3781 gagaggattc ggggacctca cgatcgagca gaaacccgag gcggaggggc gggagacgat
     3841 tgcgatcgag gcggacccca ccatcctgga ccgcgtcctc accgaggtcc ggggaggcac
     3901 gctggtcatc ggcttctcca tgccgtggta cgagtggctg acgtggtggc tccaatggat
     3961 cttcgtcgcc gacaagcgga tccggttccg gctcaccgcg gctcggatcg agtccgtcca
     4021 catatcggga gccggccgcg tcaccgtgcc gagcctccgg tgcaaccaac tggacgtggg
     4081 gatcagcggc gcggggaaga tcgttgtctc cgatatcgcc gccacggagg tccagaccag
     4141 ggtcagcgga gcgggaaaca tcgagctgac cggcgccgcg agccgtctgg aagcccggat
     4201 cagcggcgcg gggaacatcc gcgccgagga tctctccacg agacggacca tagcctccat
     4261 ctccggctcc ggcaggctca ccgccaacgc caccgaggag ctggacgccc acatcagcgg
     4321 cgccggcggg gtgggctatc gcggcaaccc gaaggtcatc cagcgcgtct cgggcgcggg
     4381 gaaggtctgg gccatccggt aggccgacgc cggctggcac ggaagcgacg ggctcgggcg
     4441 caggcccccg gcggctctcc gggtggatca gccgaaggat caggtcgtag atctgcacag
     4501 cgacgtgccg tcgtaccgcg tccagcaccc gaacggcgtc gcgttggagg ccattgcgcc
     4561 gatgtcctgc atcgggaaga cgcatgcgcc cgccgcctca tccctcgcag gatagcggcc
     4621 ggtgtccggc gcggtcaagg cgcgcgtgag ccgcgcagcc ggtggagccg gcgcaagcgg
     4681 ccgccggttt caggccgcca atccttgcgc tgccccgggc ccgcggctat agtctcccgc
     4741 atggaaacca tgaagagaac cgtcacctgc ggcgatctgc gagcatccga cgccgcgaag
     4801 tcggtgaccc tgaacggctg ggttcaccgg catcgcgacc acggcgggat ccgctttctg
     4861 gacatccggg accgctacgg agtgactcag gtggtcgtgg acgcggacgc ggccgaggat
     4921 ctgcggtccg tggcggcgga gctcaagttc gagtactgcg tggcggtgga gggcaccgtg
     4981 cgccagcgcc ctgcttccat ggccaacccc ggcatggcca cgggagagat cgaggtcaag
     5041 gcgagccgca tcgtcatcct gagccgctgc gacgtcctcc ccttcatggt ggacgagcgg
     5101 gcggatgccc gtgaggatac gcgccttcgc taccgctatc tggatctgcg gaccggggcc
     5161 atgcagcgga acatagcgct tcgccatcgg gctgccttcg caacgcggga gtatctcacc
     5221 cagcgcgggt tcttcgagat cgagacgccg atgttcatca agtccacccc ggagggcgcg
     5281 cgggactacc tggtaccgtc acggctcaac ccgggcaagt tctacgcgct gccccagtcg
     5341 ccgcagctct acaagcagct cctgatggtc tcggggttcg accgttactt ccagatcgcc
     5401 cggtgctttc gggatgagga cgcgcggggt gaccgacagc cggagttcac tcagatcgac
     5461 atcgagatga gcttcgtctc tcgcgacgac gtctttgccc tggtcgaagg gctgatggcg
     5521 cacattgtga agcagtccat cggcaaggac ctggccaccc cgttccagcg tctgccctat
     5581 gacgaggcga tgagcctcta cggctccgac aagcccgacc ttcggttcga cctcgccatg
     5641 aaggactttg gccccttcgc gctcaagggc gggttcgaag tcttccgcaa ggtggcggag
     5701 ggaggcggcg tggtgaaggc gctcgtggcg ccgggctgcg gggagtactc ccgcaagcag
     5761 atcaccgacc tggaggccgt ggcgaaggtg ttcggcgccg ccggcctcgc ctggatgaag
     5821 gtcaccgccg ccggtctgga aggcggcgtg tccaagttct acgcggagca ggccgcccag
     5881 atcccggctg ccctgggcgc gaaggccg
//
LOCUS       DASRZS010000471         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3716624, whole genome shotgun sequence.
ACCESSION   DASRZS010000471 DASRZS010000000
VERSION     DASRZS010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3716624"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VFH83_11525"
     CDS             complement(<1..178)
                     /locus_tag="VFH83_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HET6487044.1"
                     /translation="MEHGSHESQEAVALLKTARGQIDAILRMVEEDRYCIDVSKQVHA
                     SMALLKKANIVILKQ"
     gene            complement(188..>2120)
                     /locus_tag="VFH83_11530"
     CDS             complement(188..>2120)
                     /locus_tag="VFH83_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HET6487045.1"
                     /translation="ARIPLPFFLDPMRVPLQYALAQILLVIPAVIAGNRFYRVGAKAI
                     WHLAPNMDSLIAIGTAAAILYSLYNTVRVALGDAAAVENLYFETASVILTLILLGKTL
                     EAVSKGKTSQAIKKLMGLAPKTATVIHDGTETEIPISEVEVGDIVRVRPGEKVPVDGE
                     VTEGYTAVDESMLTGESIPVEKRPGDPITGASINKNGTITFRATRVGSDTALAQIIRL
                     VEQAQGSKAPIAQLADVVSGYFVPIVILIALLAGAAWLVSGQSVAFSMTIFTAVLVIA
                     CPCALGLATPTAIMVGTGKGAENGILIKGGAALEATHQIQTVVLDKTGTVTEGKPVVT
                     DVALADGIGEAALLQLAASAEKGSEHPLGDAIVASAESRGLAFLPLERFQALPGLGIE
                     ARIQGKALLLGNEKLMAQKGIPLGALAERSSRLAQDGKTPMFVAVDGAPAGIIAVADV
                     PKASSAGAIARLRRMGLEVVMLTGDNQRTAQAIARQVGVDRVLAEVMPQEKAVAVKRI
                     QAEGRRVAMVGDGINDAAALAQADIGIAIGSGTDVAIESADIVLMKSDLDDVAGAIQL
                     SKGTIRNIRQNLFWAFCYNVLGIPIAAGLLRLFGGPLLNPIFAAGAMSLSSVSVVSNA
                     LRLRRLRIRRADPAATAAA"
BASE COUNT          318 a          737 c          694 g          371 t
ORIGIN      
        1 gctgcttgag gatcacgatg ttcgccttct tgagcagggc catggacgcg tgcacctgct
       61 tggacacgtc gatgcaatat cggtcttcct ccaccatcct taatatagcg tcgatctggc
      121 ctcgtgcggt cttcagcaat gcgaccgcct cctggctctc gtgacttccg tgctccatgg
      181 gattctccta tgcggctgcc gtggcggcgg ggtcggcgcg gcggatgcgc aaccggcgca
      241 gccgaagcgc gttgctcacc acggagaccg agctcaggga catggccccg gctgcgaaga
      301 tgggattgag gagcgggccg ccgaacaggc gcaggaggcc tgccgcaatc gggatcccca
      361 ggacgttgta gcagaatgcc cagaagaggt tctgccggat gttgcggatg gtccccttgc
      421 tgagctggat ggcgccggcc acgtcgtcca ggtcgctctt catgaggacg atgtccgcgg
      481 actcgatggc cacgtccgta ccggatccga tggcgattcc gatgtccgcc tgcgccagcg
      541 cggccgcgtc gttgatgccg tccccgacca tcgccacgcg ccgcccttcc gcctggatcc
      601 tcttcacggc caccgccttc tcctgcggca tgacctccgc caggacccgg tccacgccca
      661 cctgccgggc gatggcctgg gccgtcctct ggttgtcccc ggtgagcatc accacctcca
      721 ggcccatacg gcgcagccgg gcgatggctc ccgcgctgga cgccttgggc acgtccgcca
      781 cggcaatgat ccccgccggc gcgccatcta cggccacgaa catcggcgtc ttgccgtctt
      841 gggcgagacg ggaggaccgc tcggccagcg cccccagcgg gatccccttc tgggccatga
      901 gcttctcgtt gcccagaagg agcgccttgc cctggatgcg ggcctcgatc cccagcccgg
      961 gcagcgcctg gaaccgctcg agcggaagga aggcaagccc tcggctctcg gcgctcgcca
     1021 cgatggcatc ccccagcggg tgctccgagc ccttctccgc ggaggcggca agctgcagca
     1081 gcgccgcctc cccgatcccg tccgcgagcg ccacgtccgt gaccaccggc ttgccttccg
     1141 tgaccgtgcc ggtcttgtcc agcaccacgg tctggatctg gtgggtcgcc tccagggccg
     1201 cgccgccttt gatcaggatc ccgttctccg cgcccttgcc ggttcccacc atgatggccg
     1261 tgggcgtggc cagaccgagg gcgcaagggc aggcgatgac cagcaccgcc gtgaagatgg
     1321 tcatgctgaa ggccacgctc tggccggaga cgagccacgc ggcgcccgcc aggagcgcga
     1381 tcaggatcac gatgggcacg aagtaacccg acaccacgtc cgcgagctgc gcgataggag
     1441 ccttggaccc ctgggcttgt tccaccaggc ggatgatctg cgccagcgcc gtgtcgcttc
     1501 ctacgcgggt tgcgcgaaag gtgatggtcc cgttcttgtt gatgctggcg ccggtaatcg
     1561 gatctcccgg ccgcttctcc accgggatgc tttcaccggt cagcatggac tcgtccaccg
     1621 cggtgtagcc ttcggtgacc tcgccgtcca ccggcacctt ctctccgggc cggacccgga
     1681 cgatgtcacc cacctcgacc tcggagatcg ggatctccgt ctccgtcccg tcatggatca
     1741 cggtggcggt cttgggcgcc aggcccatca gcttcttgat ggcctgggac gtcttcccct
     1801 tggataccgc ctccagcgtc ttgcccagga ggatcagcgt gaggatgacc gacgcggtct
     1861 cgaagtagag gttctcgacg gcggccgcgt cgccgagggc gaccctcacc gtgttgtaga
     1921 ggctgtagag gatggcggcc gcggtgccga tcgcgatcag ggagtccatg ttcggcgcca
     1981 ggtgccagat ggctttggcg cccacccgat agaaccggtt gcccgcgatc acggccggga
     2041 tcaccagaag gatctgggcc agcgcgtact ggagtggaac gcgcatggga tccaggaaga
     2101 agggaagggg gatccgcgcg
//
LOCUS       DASRZS010000472         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4488482, whole genome shotgun sequence.
ACCESSION   DASRZS010000472 DASRZS010000000
VERSION     DASRZS010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4488482"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1377
                     /locus_tag="VFH83_11535"
     CDS             196..1377
                     /locus_tag="VFH83_11535"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET6487046.1"
                     /translation="MAKKTVRDVDLRKKRVLVRVDFNVPLKDGSITDDTRITAALPTL
                     NYILDQKGASLVLMSHLGRPKKPDKENSLAPVAARLGELLRRKVTLAPDCIGPAAEAA
                     VKALKPGEVVLLENVRFHPEEEKNDPAFARQLAALGDVYVNDAFGTAHRAHASTEGVA
                     HILPGVAGFLMEKEIKFLGGVFENPARPFVAIIGGAKVSTKIAVLESLLPRVTTMIIG
                     GGMAYTFLKVQGHAVGGSLVEDDFAQTASGLLAKATQAGVEVLLPVDHKVGSEFSESA
                     KAEAVDGLEIPAGKVAMDIGPKSVALFRERIVKARTVIWNGPLGVFEFPAFAAGTQEI
                     AKAVADCKGTTVVGGGDSVAAVNQFGLADRITHVSTGGGASLELLEGKQLPGVVALQD
                     K"
     gene            1383..>2130
                     /gene="tpiA"
                     /locus_tag="VFH83_11540"
     CDS             1383..>2130
                     /gene="tpiA"
                     /locus_tag="VFH83_11540"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET6487047.1"
                     /translation="MRKAFIAGNWKMYKTASEASALASALVSALAADSEPKVMIAPPF
                     TALHVVREALRGSRILLGAQNAASEVEGAHTGEISPRMLKDAGVSVVILGHSERRHVY
                     GETDALVNKKVKLALTQGLDVILCVGETLDERQSGVTENVVQRQTEAGLAGVAAAELG
                     RVTVAYEPVWAIGTGKNATPQDADAVHSFLRSVVSRMYTSEAAQSLIIQYGGSVKPEN
                     IKDLMRMENIDGALVGGASLKAETFLPIVRY"
BASE COUNT          386 a          659 c          728 g          357 t
ORIGIN      
        1 atctcagtca gccccctcga ttgacactcc cggaccattc ccgtatagtt ttgccgattg
       61 cgccacagcc atgcatcgtc gcggcgttcc catccccagg cgcgacggcg gaatcgccgg
      121 caaccgtagg agcccggaag cgcccaaggg ttcgagacgc cgtgacgacg gtaagcgtgt
      181 cccgaggagt tccaaatggc gaagaagact gtccgcgatg ttgacctccg caagaagcgg
      241 gtccttgtga gggttgactt caacgtgccg ctgaaggacg gatccatcac ggatgacacc
      301 cgcatcacgg cggcgctccc cacgctgaac tacatcctgg accagaaggg agcctccctt
      361 gtgctgatga gccacctggg gagacccaag aagccggaca aggagaacag cctggcgccc
      421 gttgcggctc ggctgggaga gctcctgcgg cgcaaggtga ccctggcccc ggattgcatc
      481 gggccggcgg cggaagccgc ggtcaaagcc ctgaagccgg gagaggtggt gctcctggaa
      541 aacgtccgct tccacccgga agaggagaag aacgatcccg cgtttgccag gcaactggcc
      601 gcgcttgggg atgtttatgt gaacgacgcg ttcgggaccg ctcatcgcgc tcacgcctcc
      661 accgaaggtg ttgctcatat cctgcccgga gttgccggat tcctcatgga gaaagagatc
      721 aagttcctgg ggggcgtttt tgagaatccc gcgcgcccgt tcgtagcgat catcggcggc
      781 gccaaggtgt ccaccaaaat cgccgtcctg gagtcgctgc ttccccgggt gaccacgatg
      841 atcatcggcg gcggcatggc ttacacgttc ctcaaggtgc agggacacgc cgtcggcgga
      901 tcgctggtgg aggacgattt cgcccagacc gcttccggcc tgctggccaa ggccacccag
      961 gccggcgtgg aggtgctcct cccggtcgat cacaaggtgg ggagcgagtt ctccgagtcc
     1021 gcgaaggcgg aggccgtgga cggcctggag atcccggcgg gcaaggtcgc catggacatc
     1081 ggtcccaagt cggtggcgct gttccgggag cgcatcgtca aggcccggac cgtgatctgg
     1141 aacggcccgc tgggggtttt cgagttcccg gcgttcgctg ccggtacgca ggagatcgca
     1201 aaggccgtag cggactgcaa ggggacgacg gtcgtcggtg gcggcgattc ggtcgccgcg
     1261 gtcaaccagt ttggactggc ggatcgaatc acgcacgtct cgacgggcgg aggcgcctcc
     1321 ctggagcttc tcgaaggcaa gcagctcccc ggggtcgtgg ccctccagga caagtgatcg
     1381 acatgcgcaa agcgttcatc gccggtaact ggaagatgta caagacggcc tcggaggcgt
     1441 ccgccctggc cagcgctctg gtgtccgccc tggccgcgga ctcggagccc aaggtcatga
     1501 tcgctccgcc gttcacggcg cttcatgtag tgcgggaagc cctccgcggc tcccggatcc
     1561 tgctgggcgc gcagaacgca gcctcggagg tggagggcgc ccataccggg gagatctcgc
     1621 cgcggatgct gaaggatgcg ggcgtgtccg tcgtcatcct cggccattcc gagcggcgcc
     1681 acgtctacgg agaaaccgac gcgctggtca acaagaaggt caagctcgct ctgacccagg
     1741 ggctcgacgt cattctctgc gtcggggaga cgctggacga gcgccagtcc ggggtgacgg
     1801 agaatgtggt gcagcgacag accgaggcag ggctggcggg cgttgccgct gccgagctgg
     1861 gacgcgtcac cgtcgcctac gagccggtgt gggccatcgg gaccggcaaa aacgccactc
     1921 cccaggacgc ggatgcggtg cattccttcc tgagaagtgt ggtttcccgg atgtatactt
     1981 cagaagcggc ccagagcctc atcatccagt atggcggctc ggtgaagccg gagaacatca
     2041 aagatctgat gcggatggag aacattgacg gcgcgctggt gggcggcgct tcgctcaagg
     2101 cggagacgtt cctgcccatc gtgcgctacg
//
LOCUS       DASRZS010000473         6015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2949182, whole genome shotgun sequence.
ACCESSION   DASRZS010000473 DASRZS010000000
VERSION     DASRZS010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2949182"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFH83_11545"
     CDS             complement(<1..236)
                     /locus_tag="VFH83_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487048.1"
                     /translation="MSAPAGSLFRGWIETARKVALFLVLVIGSAALGAAIAWPLWRFA
                     TAARGAYTWFALGVVGAGIVAAATRGAARRRRLPP"
     gene            complement(233..1171)
                     /locus_tag="VFH83_11550"
     CDS             complement(233..1171)
                     /locus_tag="VFH83_11550"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6487049.1"
                     /translation="MTRLAGRMRWATCAVCACMALASCSTAPARTDTVTAVKKQAADA
                     SAVGDGYFHQGRYDLAIQFFDQALSYSTSVDDQAGIVLASIAIGKVYMTAGSLDDAER
                     ILAEARDRARAADPRLLFLSSINLGELYLRKARPQDALAVFEQAKTMPDSARTSAQNA
                     ILLHDMGTARKSLDDLPGALGLFTQSLKINLDLKLGAEAASDYYMIASVYSKQGDYDN
                     AARNAQLALEADKKVENAPAIAQDLYALGLIDRKRQGFSAAYDWFLRSYQVSLALSMR
                     AEMRKALTEMASCADALGNGEDAESARKLLADLGNP"
     gene            complement(1171..2298)
                     /locus_tag="VFH83_11555"
     CDS             complement(1171..2298)
                     /locus_tag="VFH83_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET6487050.1"
                     /translation="MKLKIRFAEQIVGSFLLVAILGVVALLVLIGVNQRWFARNYLFT
                     SRFASADGLAVGMPIMLKGFEIGKITRISLNEQDEVDIQFLVEDTYYDRIRPNSVLQL
                     VSSPIGLGTSLKFIPGKDAAPPLPEMAFVPTVDSDEGKALVAAGLVVLPKGEDVIGTV
                     ISKINPVLDDARDTIVQIRRLVDSANLAFSGKGGPVGTMVSDLSGTPARVNQAVTDAS
                     TRINDLLDRVSEVSDSAQKIATHTDATIQQLSDNLETITDNLKEMSAELKNTQGLAKR
                     LLDPKGSVDTLLNDQNQLYDQIDQAVKGVDGIVTQIKGFVDFVNGSRPEISSILEKGR
                     TTLDNTNDVLQAAKNNPLLKGGVPPAPVQSSPTQGYRDEDF"
     gene            complement(2336..3154)
                     /locus_tag="VFH83_11560"
     CDS             complement(2336..3154)
                     /locus_tag="VFH83_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6487051.1"
                     /translation="MDRQEARSAIEMEQVTVIHGDTVALSDVTVDFPASTSTVVMGPS
                     GCGKSTLLKVAAGLIPPDRGRVCFRGMDVFTMTGRAEREFRRANGFVFQDGALWENKT
                     VAENLALPLEVHFESLHPSEVYRRVLHALERVGLTAAASHRPASLSSGERKIVSLLRA
                     LITEPSVVFLDEPTLSIDHSMSDRIRQMIHELKARGCTIITVTEDPRLASTLADRLVV
                     LDAGLLLEAGDFDQVKRTRNARARSILSQVLGEIASYDTDLLGLLEGDESLPEN"
     gene            complement(3173..3949)
                     /locus_tag="VFH83_11565"
     CDS             complement(3173..3949)
                     /locus_tag="VFH83_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487052.1"
                     /translation="MVGALGRWATQRIRDYSYAMGFLFQVGRETAFFMRRKQVAFRVL
                     VLQILFTGFEALGIVSLIALSIGAVIIVEGGSVLPRFGQTSLMYSILIVVITRELGPI
                     LTAFVIIARSGTAIATELGNMVVSHEIEAYLSVGINPVSYLVVPRVLGVTISMIALTI
                     YFNAFGLIGSFLVSQLVKPVPFSEYFRALLRTLQLRDILSTLLKSVVFGIIVSVTATF
                     QGFRVRNSVTEIPRAAIRAVGQGFVLCFLADAIITLIYYL"
     gene            complement(3965..4807)
                     /locus_tag="VFH83_11570"
     CDS             complement(3965..4807)
                     /locus_tag="VFH83_11570"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET6487053.1"
                     /translation="MIDLHSHSTASDGGCTPTRLVELALGIGIRALALTDHDTVAGVP
                     EASERAEGTGTLLIPGVEVEIEVPTGEFHLLGLGLFGDRRPLDAALDRVREARRGRNA
                     RMVDKMRDGGIPITLEEVSAIAGGEIVSRAHFARLLVRKRVVSSVDAAFSRLIGKGCP
                     YYEPRACLELREAVQLIETAGGFTSVAHPASLGLTEPALRQHLSGCRDLGVRGLEAWH
                     PNHTLKFCHRLEKLARSLGMLVTGGSDFHGEHMPQRRLGQSSGGRPVPDMLLETLPLH
                     PAGR"
     gene            4844..>6015
                     /gene="aroA"
                     /locus_tag="VFH83_11575"
     CDS             4844..>6015
                     /gene="aroA"
                     /locus_tag="VFH83_11575"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET6487054.1"
                     /translation="MDALFRAPRGLEGGMEAPADKSITHRALMLAAVSSGRCRIGNPL
                     ATGDCVSTRRCLEALGVAIEQADGGLVIQGVGLRGLREPKGPLDAENSGTTTRLLSGL
                     LAGQRLFAVLTGDDSLRRRPMARVVEPLRAMGARVEGREGGTLAPLCVLPGSGDLLPA
                     SYRLAVASAQVKSCLLFAGLRASSASRITGEVASRDHTERMLRALGVRIEEKDGEVLV
                     HPPSSLPGFETRVPGDVSSAAFFIAASVLSGKPVTVRRCGLNPTRLGFLSVVRRMGAR
                     VDQAEAEATLGEPVGALRVDADGLRGTSVPSSEIPELIDEVPLIAVLGLFARGRTEVR
                     GAQELRYKESDRLRAVAEMTAALGGRIEIRDDGFVVEGPQDLRTGVVDPHGDHRIA"
BASE COUNT         1004 a         1974 c         2050 g          987 t
ORIGIN      
        1 ggcggcaggc gccgccgccg cgccgcccca cgtgtggccg cggccacaat accggccccg
       61 acgaccccga gcgcgaacca tgtgtaggcc ccccgggcgg ccgtggcaaa gcgccagagc
      121 ggccaggcga tcgcggcccc cagcgccgcg cttccgatca ccaggacgag aaagagcgca
      181 acctttctcg cggtctcgat ccagcccctg aagagcgatc ctgccggcgc gctcacgggt
      241 tccccaggtc tgccaggagc ttccgagcgc tctccgcgtc ctccccgttg cccaacgcat
      301 ccgcgcagga cgccatctcg gtcagggcct tgcgcatctc ggcccgcatg gagagggcca
      361 gggagacctg ataggagcga aggaaccagt cataggcggc ggaaaaccct tgccgcttgc
      421 gatcgatcag gcccagggcg tagaggtcct gggcgatggc gggggcgttc tcgaccttct
      481 tgtccgcctc cagggctagc tgagcgttgc gagcggcgtt gtcgtagtct ccctgcttcg
      541 agtagaccga cgcgatcatg taatagtccg acgcggcctc cgcgcccagc ttgaggtcca
      601 ggttgatctt cagagactgc gtgaacagcc caagggcgcc ggggaggtca tccaggctct
      661 tgcgggcggt tcccatgtcg tgcaggagga tggcgttctg cgccgaggtc ctcgcggaat
      721 ccggcatggt cttcgcctgc tcgaacacgg caagggcgtc ctggggccgg gccttccgga
      781 ggtagagctc gcccaggttg atggaggaga ggaacaggag cctgggatcc gccgctcggg
      841 cgcggtctcg cgcctcggca aggatgcgct ccgcgtcatc cagcgagccc gcggtcatgt
      901 agactttgcc gatggcaatg gacgccagaa cgatgccggc ctggtcgtcg accgaggtgc
      961 tgtacgaaag cgcctggtcg aagaactgga tggcaagatc gtatcgtccc tggtggaagt
     1021 agccgtcccc tacggcgctg gcatccgccg cctgcttctt caccgccgtt acggtgtccg
     1081 tccgggcagg ggccgtcgag caggaggcga gcgccatgca ggcgcacacg gcgcaggtcg
     1141 cccaccgcat cctgccggca agccttgtca tcagaaatcc tcgtcccggt acccttgggt
     1201 cgggctgctc tgaaccgggg ccggtgggac cccacctttg agcagagggt tgttcttggc
     1261 cgcctggagc acatcgttgg tgttgtcgag cgtggtccgg cccttttcca ggatgctgga
     1321 gatctccggc cgggatccgt tcacgaaatc gacgaacccc ttgatctgcg tcacgatgcc
     1381 atccacgcct ttcacggcct gatcgatctg gtcgtagagc tggttctggt cgttcagcag
     1441 ggtatccacg gagcccttcg ggtccaggag ccgcttggcc agaccctgcg tgttcttcag
     1501 ctcggcgctc atctccttga ggttgtcggt gatggtctcc aggttgtcgg agagctgctg
     1561 gatcgtggca tccgtgtgcg tggcgatctt ctgggcgctg tcggacactt cggagacccg
     1621 gtccaggagg tcgttgatcc gcgtggacgc gtccgtcacg gcctggttga cgcgggccgg
     1681 cgtccccgag agatcgctta ccatcgtccc caccggccct cccttgccgg agaaagccag
     1741 gttggcggag tccaccagcc tgcggatctg gacgatggta tcccgcgcat cgtcgagcac
     1801 cggattgatc ttggagatga ccgtgccgat gacgtcctct cccttgggta ggacgaccag
     1861 cccggcagcg acgagtgcct tgccttcgtc gctgtctacc gttgggacga aggccatctc
     1921 cggcagcggc ggagccgcat cctttccggg gatgaacttc agggatgtgc cgaggccgat
     1981 cgggctggag acgagctgga gcacggagtt gggccggatc cggtcgtagt acgtgtcctc
     2041 caccaggaac tggatgtcca cctcgtcctg ctcgttcagg gagatccgcg tgatctttcc
     2101 gatctcgaac cccttgagca tgatcggcat ccccaccgcg agcccgtccg cggaggcgaa
     2161 ccggctggtg aagaggtagt ttcgcgcgaa ccatcgctgg ttgacgccga tcagcacgag
     2221 gagggccacg actcccagga tggccacgag caggaaggag ccgacgatct gctcggcgaa
     2281 acggatcttc agcttcatgc gctttccgtg atcctaaacg ccgccgcggg agctgtcaat
     2341 tctcgggcag cgattcgtct ccctccagca gacccaggag gtccgtgtca taggaggcga
     2401 tctctccgag cacctgggag aggatggacc gggcgcgcgc gttgcgggtc cgcttcacct
     2461 ggtcgaaatc cccggcctcc agcaggagcc cggcgtccag caccacgagc cgatcggcga
     2521 gggtggaggc cagccgaggg tcctccgtga ccgtgatgat cgtgcacccc cgagccttga
     2581 gctcgtggat catctggcgg atccgatcgc tcatcgagtg gtcgatggag agcgtcggct
     2641 cgtccaggaa gaccaccgaa ggctccgtga tgagcgcgcg gaggagggac acgatcttgc
     2701 gctccccgct ggagagcgag gcgggacgat gcgaggctgc cgccgtcagc cccacccgct
     2761 ccagggcgtg caggaccctc cgatagacct cggacgggtg caggctctcg aagtggacct
     2821 cgaggggaag ggccaggttc tcggccacgg tcttgttctc ccacagggcg ccgtcctgga
     2881 agacgaagcc gttggcgcgg cggaactccc gttctgcgcg accggtcatg gtgaacacgt
     2941 ccatgccgcg gaaacagacc cgtccgcggt ccggagggat gagacccgcc gcgaccttga
     3001 gcagcgtgct cttgccgcac ccggagggtc ccatcaccac ggtgctggtg gaggccggaa
     3061 aatcgacggt cacgtcgctc agcgctacgg tatccccgtg gatcaccgtg acctgctcca
     3121 tctcgatcgc gcttctggcc tcttgtcggt ccacggatcc cccggcagcg ccctaaagat
     3181 agtagataag cgtgatgatc gcgtctgcca ggaagcagag cacgaatccc tgccctacgg
     3241 cgcggatggc cgctcggggg atctcggtca ccgagttgcg cacccgaaag ccctggaagg
     3301 tggccgttac ggacacgatg atgccgaaga ccacgctctt gagaagcgtg gacaggatgt
     3361 cccgaagctg cagggtccgc aggagcgcgc gaaagtactc cgagaagggg accggcttga
     3421 cgagctggga caccaggaag gacccgatca ggccgaaggc gttgaagtag atggtgagcg
     3481 ctatcatgga aatggtcacc cccagcaccc gggggaccac gagatacgag acggggttga
     3541 tgcccaccga gagataggcc tcgatctcat gggaaaccac catgttgccc agctccgtag
     3601 caatcgccgt tcccgagcgg gcgatgatca cgaacgcggt gaggatggga cccaactccc
     3661 gggtgatgac cacgatcagg atcgagtaca tgaggctggt ctggccgaac cggggcagga
     3721 cggatccccc ttccacgatg atcacggcgc cgatggaaag ggcgatcagg gaaacgattc
     3781 ccagggcctc gaaccccgta aagaggatct gcaggacgag cacccggaag gccacctgtt
     3841 tccggcgcat gaagaacgcg gtctccctcc cgacctggaa gaggaacccc atcgcgtagc
     3901 tgtagtctcg gattctctgg gtggcccagc ggcccagcgc cccgaccatg ggcgccagta
     3961 tagttcagcg tccggcggga tgcaacggaa gcgtctccaa cagcatgtcc ggtacgggcc
     4021 ggccgccgct gctctgcccc aaccgtcgct ggggcatatg ctccccgtga aagtcggagc
     4081 cgccggtcac cagcatcccc agggaacgcg ccagcttctc cagacgatgg cagaacttga
     4141 gcgtgtggtt gggatgccac gcctccagac cgcggacgcc gaggtcccga cacccgctca
     4201 ggtgctggcg aagcgccggc tccgtcagcc ccaaggacgc cggatgcgcc accgacgtga
     4261 acccgccggc agtctcgatg agctgtaccg cctcccggag ctccaggcat gcccgaggct
     4321 cgtagtaggg gcagcccttg ccgatcagcc tggagaaggc ggcgtcgacg gagctcacga
     4381 cgcgcttgcg caccagcaga cgagcgaagt gcgcccggct cacgatctct ccccccgcga
     4441 tggcggagac ctcctccagc gtgatgggaa tgccgccgtc ccgcatcttg tctaccattc
     4501 gagcgttgcg gccccgtcgc gcctcccgca cgcgatccag ggcggcgtcc aggggtcgcc
     4561 ggtccccgaa caacccgagc cccagcaggt ggaactcgcc ggtgggcacc tcgatctcca
     4621 cttcgacccc gggaatgagg agcgtgcccg ttccttccgc tcgctcggac gcctcgggta
     4681 cgccggccac cgtgtcgtgg tccgtcagcg ccagcgcccg gatgccgata ccgagcgcca
     4741 gctccaccag gcgggtcggc gtgcagccgc cgtccgaagc cgtggagtgg gagtgcaggt
     4801 cgatcatttg acaggaatat agcgaattgc ataagctcga accatggacg cgttgttccg
     4861 agcgccgcgc gggctcgagg gcgggatgga ggcccccgcg gacaagtcga tcacccaccg
     4921 cgcgctgatg ctcgcggcgg tctccagcgg gcgctgccgg atcggcaacc cgctggccac
     4981 gggtgactgc gtgtccacgc gacgctgtct ggaagccctc ggcgtcgcca tcgaacaggc
     5041 cgacggcggc ctggtgatcc agggggtcgg cctgcgtggg ctgcgggagc ccaagggccc
     5101 gctggacgcg gagaactccg ggaccaccac gcggctgctc tccggtcttc tggccggaca
     5161 acgcctcttc gccgtgctca cgggggacga ctcgctgcgc cgccgaccca tggcgcgggt
     5221 cgtggagccg ctccgggcca tgggggcccg ggtcgagggg agagaagggg gaacgttagc
     5281 gcccctctgc gttctacccg gcagtgggga cctgctcccc gcgtcctacc ggcttgcggt
     5341 ggcaagcgcc caggtaaaga gctgccttct ctttgccggg cttcgggcgt cctctgcctc
     5401 ccgcatcacc ggtgaagtgg cgtcacgaga ccacacggag cggatgctgc gcgcgctggg
     5461 cgtccgcatc gaagagaagg acggcgaggt gctggtgcat cccccttcgt ccctgccggg
     5521 attcgagacg cgggtccccg gtgacgtctc ctcggcggcc ttcttcatcg cggcatcggt
     5581 cctgagcggc aagccggtga cggtgcgccg ctgtggcctc aaccccacgc ggttgggatt
     5641 cctgtcggta gtgaggcgga tgggcgcccg cgtggaccag gccgaggcgg aggccaccct
     5701 cggcgagccc gtaggggcgc tccgggtgga cgcggacggg cttcgaggga ccagcgtccc
     5761 atcctccgag attccggagc tgatcgacga ggtaccgttg atcgccgtcc tggggctctt
     5821 cgcgcgcgga aggaccgagg tccgaggcgc ccaggagcta cgctacaagg agagcgaccg
     5881 cctgcgggca gtggccgaga tgacggcggc cctgggtgga cgcatcgaga ttcgggacga
     5941 cgggttcgtc gtggaagggc cgcaggatct gcgcacgggg gtggtggacc ctcacggcga
     6001 tcaccgcatt gcgat
//
LOCUS       DASRZS010000474         4557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4874491, whole genome shotgun sequence.
ACCESSION   DASRZS010000474 DASRZS010000000
VERSION     DASRZS010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4874491"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="VFH83_11580"
     CDS             complement(<1..991)
                     /locus_tag="VFH83_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6487055.1"
                     /translation="MRSAGSARTPTIRDVARIAGVSIQTVSAVMNGKPGITEDTAARV
                     RRAIAEVGYRPHSLAASLRTRKTRTIALIVSDIANPSLAVMASAAERYAQSMSYAVVY
                     YNTHDDLEREKESIRLAAQRWVDGIVVVSAGDHMPSYAETLSFPGMHCVPIERIPEGY
                     TGPCVGFDNVEAGRIAARHLLELGHTRLAHVSGPLALAPARDRCTGFQLELTVHGLPA
                     PPCVDEGCTWDCESGYRSMRRILAMQPRPTAVFAANDRLAVGAMLAAHQQGLRIPADL
                     SVVGLDDFETARYHIPPLTTIAQPFSEIATSALRLLFELLAGNQPATPQIILKP"
     gene            1365..3233
                     /locus_tag="VFH83_11585"
     CDS             1365..3233
                     /locus_tag="VFH83_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6487056.1"
                     /translation="MAKKSLVFLIAAVLLAGLAVQTTFAGESPMLAALVKAGKLPALK
                     SRLPDQPYVVHTLGGAAKAVYGGQLQLIRVDPFLDPDGGYPNAMNDTILVMPGWDGTY
                     NATGNRPMPHLVADFKVSSDTKEYTFTLRKGMKWSDGQPFTSDDFAFAWNDVLLDTNI
                     TPVFPKAFSTAGGVPTLTVVDPLTFKITFKDSYGGFAELLTTTFNYPALFLPKHYLMQ
                     FLPKYADSGKLAQAIADAKYNSGEYWRLFIQKRDTDPGIPGVTPWIITDRSGGFITWV
                     RNPYYWKVDELGNQLPYLDGMKSTLIRDPTMAPLKALAGEVDFLREQANLTDVPLYKQ
                     YEKKGGYTTVLYNKDVETGEVLINMSYQDPVWDKVVQDVRFRQALSLAINRDELIKGV
                     YLGQAVPGGYVDPTYDPAKANQLLDQMGLDKKDANGMRLGPDGNKFTIPFEIPTLMGF
                     EVPATTMVIDYWKKVGIDATMKTVTFALWDQLVRGNTIHATIHWMDPAYDVRATPAKS
                     GGPFGRYSGSWAPAWNDWHDSNGTQGLEPPDLIKQLFKDRDTALTGNDTQRIAAGADE
                     LKIIHDNYLWLGLTQAKAPIVYSARLMATTPATNSAGVGESSFKPFVMLVQAYFKK"
     gene            3322..4320
                     /locus_tag="VFH83_11590"
     CDS             3322..4320
                     /locus_tag="VFH83_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487057.1"
                     /translation="MVNLIGKRLLILIPLLLVISVVAFIIIQLPPGSFLDMYVQQLTT
                     QGTNVSQDQIAALTKQYGLDKPMIVQYWIWISNIVFHGNFGLSFQWNKPVSTLIGERI
                     ILTMVISLLSTVFIWLVSVPIGIYSATHQYTAGDYAVTVLGFIGIATPGFVLALVFLW
                     VAFKGFGVAVTGLFSAPYADAPWSLGKVLDMLKRIWVPVILTGLSGTAYLIRVLRGTL
                     LDELKKQYVITARAKGLSEVKLLFEYPVRVAINPIISTIGWMLPGIISGELLVSIVLN
                     LPTAGPLLWGALLSQDMFLAGSFLLILSALTVVGTLISDILLAAVDPRIRYKGAVK"
     gene            4317..>4557
                     /locus_tag="VFH83_11595"
     CDS             4317..>4557
                     /locus_tag="VFH83_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487058.1"
                     /translation="MSAGVSRSDSPGSDAPGSDAPRSEASDKIRSEVTIQRSADSAQP
                     PDQEEREERYYMAGQAQLIWMKFRRHKLAIVAMVVL"
BASE COUNT          823 a         1545 c         1406 g          783 t
ORIGIN      
        1 tcggcttcag aatgatctgg ggagtggcgg gctggtttcc cgccagcagc tcgaagagca
       61 gccgaagcgc gctcgtggcg atctccgaga acggctgggc aatcgtcgtg agcggcggga
      121 tgtggtagcg ggccgtctcg aagtcgtcca ggccgaccac ggacaggtcc gccggaatcc
      181 gaagcccctg ctgatgcgcc gccagcatgg ccccgaccgc caggcggtcg tttgccgcga
      241 atacggccgt gggccgaggc tgcatcgcca ggatcctccg catcgaccga tacccgctct
      301 cgcagtccca ggtgcagcct tcatccacgc acggcggcgc cggaagccca tggaccgtga
      361 gctcgagctg gaagccggtg caccgatccc gcgcgggggc cagcgcaagc ggaccgctca
      421 cgtgagccag ccgcgtgtgc cccagctcca gcaggtggcg ggcggcgatg cggccggcct
      481 ccacgttgtc gaagccgacg cacgggccgg tgtacccttc ggggatccgc tcgatcggca
      541 cgcagtgcat ccccgggaag gagagtgtct ccgcgtagct gggcatgtgg tctccggcgg
      601 agaccaccac gatcccgtcg acccagcgct gggccgcgag gcggatggac tccttctctc
      661 gctccaggtc gtcgtgggta ttgtagtaga ccaccgcgta cgacatggac tgggcgtagc
      721 gctccgcggc gctcgccatt acggcaagcg acgggttggc gatgtccgag acgatcaggg
      781 cgatggtccg ggtctttcgg gtccggaggc tcgcggccaa cgagtggggc cggtacccta
      841 cctccgcgat ggcgcgccgt actctggccg cggtgtcctc ggtgatcccg ggcttgccgt
      901 tcatgacggc cgacacggtc tggatcgaca cgccggcaat cctcgccacg tcccgaatcg
      961 tgggcgttct ggcgctcccc gctgacctca ctgcctcacc tttcgccgcc ccattggcgg
     1021 ggaatgccac gcctcccggc gtggccgcgc tctacgccgc cagaagcgct ggaatcagcg
     1081 tttcacacgg tcacatgaac gttcacacta tatgggcggc ggtcggggac gtcaaccggg
     1141 tgcgcgccgc cagcttctgc aggcctgagt cggcgttgac aaacgtcccc gagtatggat
     1201 aatagcgact cgctggaacg ttcacatgaa cggtccatgc acgcggaagc tcaggacccg
     1261 gaggatctga ccaatcccga tggaccggcg aaagctctcg accacccgtc aacccaagaa
     1321 accttgccgt gcccaagtct agcaaagatc gagaggagga aacgatggcg aagaagagtc
     1381 ttgtgtttct gatcgcggct gttctcctgg cggggctggc cgttcagacc acctttgccg
     1441 gagagtcgcc catgctcgcg gccctcgtga aggcgggcaa gctaccggct ctcaagagcc
     1501 gtttgccgga tcagccctac gtcgtgcata ccctgggcgg cgccgccaaa gcggtctacg
     1561 gtgggcagct ccagctcatc cgcgtggatc ccttcctgga tccggacggc ggctacccca
     1621 acgccatgaa cgacaccatc ctggtcatgc ccgggtggga cggcacgtac aacgccaccg
     1681 gcaaccggcc catgccccac ctggtggcgg acttcaaggt cagctccgac accaaggagt
     1741 acaccttcac gctccgcaag gggatgaagt ggtccgatgg tcagcccttc acctcggatg
     1801 acttcgcgtt cgcgtggaac gacgtgctcc tggacaccaa catcacgccc gtgttcccca
     1861 aggccttcag cacggccggc ggcgttccca ccctcacggt ggtggatccg ttgaccttca
     1921 agatcacctt caaggacagc tacggcggat tcgcggagct cctcaccacg acgttcaact
     1981 atcccgccct cttcctgccc aagcactatc tcatgcagtt cctgcccaag tacgcggact
     2041 ccgggaagct ggcgcaggct atcgcggacg ccaagtacaa ctccggcgag tactggagac
     2101 tgttcatcca gaagagagac acggaccccg ggatccccgg cgtgaccccg tggatcatca
     2161 cggaccgcag cggcgggttc atcacctggg tccgcaaccc ctattattgg aaggtcgatg
     2221 agctgggcaa ccagctcccg tatctcgacg ggatgaaatc cacgctgatc cgggatccca
     2281 ccatggcgcc cctcaaggcc ctggccggag aggtggactt cctgcgagag caggcgaacc
     2341 tcaccgacgt ccccctgtac aagcagtacg agaagaaggg cggctacacg acggtcctct
     2401 acaacaagga cgtggagacc ggcgaagtgc tcatcaacat gagctaccag gaccccgtat
     2461 gggacaaggt ggttcaggac gtgcgcttcc gccaggcgtt gagcctggcc atcaatcgcg
     2521 atgagctcat caagggcgtc tacctgggtc aggcggtgcc cggcgggtac gtcgatccga
     2581 cctacgatcc cgccaaggcc aaccagcttc tggaccagat gggtctggac aagaaggacg
     2641 ccaacggcat gcggttggga cccgacggga acaagttcac gatccccttc gagattccga
     2701 ccctcatggg gttcgaggtg ccggccacca ccatggtcat cgattactgg aagaaggtcg
     2761 ggatcgatgc gaccatgaag acggtcactt tcgcgctctg ggaccagctt gtccgcggca
     2821 acacgatcca cgccaccatc cactggatgg atccggcgta tgacgtgcgg gccaccccgg
     2881 ccaagagcgg cggcccgttc ggccggtatt ccggatcctg ggcgcccgcc tggaacgact
     2941 ggcacgacag caacgggacg caggggctcg agcccccgga cctgatcaag cagctcttca
     3001 aggaccggga cacggcgctc accggcaacg acacgcagcg catcgccgcc ggcgccgacg
     3061 agctgaagat catccacgac aactacctct ggctcggtct cacgcaggcc aaggccccca
     3121 tcgtctacag cgcgaggctg atggccacca ccccggccac caactccgcc ggggtcggcg
     3181 agagctcgtt caagccgttc gtcatgctcg tccaggccta cttcaagaag taacctgagc
     3241 ccttcgggaa cccgccgtcc atagaggcgg gttcccgcct tcccacgatc ccacggaacg
     3301 cggccggcag gaggagtcct catggtgaac ctgatcggaa agcggcttct gatcctcatc
     3361 cctctcctgc tggtcatctc ggtcgtcgcg ttcatcatca tccagctccc gccggggagc
     3421 ttcctggaca tgtacgtgca gcagctcacc actcagggca ccaacgtttc ccaggaccag
     3481 atcgccgcgc tcacgaaaca gtacgggctg gacaagccca tgatcgtcca gtactggatc
     3541 tggatcagca acatcgtctt ccacgggaac tttggtctgt ccttccagtg gaacaagccg
     3601 gtgagcacgc tgatcgggga gcggatcatc ctcaccatgg tcatctccct gctctccacg
     3661 gtcttcatct ggctggtctc ggtgccgatc gggatctact ccgccacgca ccagtacacc
     3721 gccggggact acgccgtcac ggtgctggga ttcatcggca tcgccacgcc agggttcgtc
     3781 ctggccctcg tctttctctg ggtggcgttc aagggcttcg gcgtggcggt gacggggctc
     3841 ttctccgccc cgtacgcgga tgccccctgg tccctgggga aggtcctgga catgctgaag
     3901 cggatctggg tgcccgtgat cctcacgggg ctctccggga ccgcctatct catccgcgtc
     3961 cttcgaggaa cgctcctgga tgagctcaag aagcagtacg tgataacggc gcgcgccaag
     4021 gggctctccg aggtcaagct cctgttcgaa taccccgtgc gcgtcgccat caaccccatc
     4081 atcagcacca tcggctggat gctccccggg atcatctcgg gggagcttct ggtatccatc
     4141 gtgctcaacc tgcccaccgc gggcccgctc ctgtggggag cgctcctgag ccaggacatg
     4201 ttcctggcgg gctccttcct cctcatcctg agcgccctga cggtggtcgg gactctgatc
     4261 tccgacatcc tgctggcagc cgtggatcct cgcatccgtt acaagggggc cgtgaagtga
     4321 gcgctggcgt gtcgcggtcc gactctccag ggtccgacgc accggggtcc gacgcaccgc
     4381 ggtccgaggc atcggacaag atccgctccg aggtcaccat ccagcggtcc gccgactctg
     4441 cgcagcctcc cgaccaggag gagcgggaag agcgctacta catggccggg caggcccagc
     4501 tcatctggat gaagttccgc aggcacaagc tggcaatcgt ggccatggtg gtcctgg
//
LOCUS       DASRZS010000475         3626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1415375, whole genome shotgun sequence.
ACCESSION   DASRZS010000475 DASRZS010000000
VERSION     DASRZS010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415375"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VFH83_11600"
     CDS             <1..907
                     /locus_tag="VFH83_11600"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET6487059.1"
                     /translation="VPARQTPGEALGKSPQGSSEAGPVASETGIALPSTPGETYDITK
                     RVDWPPTTGRKAYLSWMLAHTAETEKFLNAKWDRAQTILAIGNITHKRVLEAFLTTPR
                     EEFSRDPKRAYDNAALPIGWGQTISGPHMVSRMTDYLDPQPDQKVLEIGTGSGYQSAV
                     LSELSNHVYTIEIVEPLAQKTNAIYQSLEDRYPEYRNIQRKVDDGYYGWPQNAPFDRI
                     IVTCGIDHVPPELLRELAPEGIMVIPIGPPSGQTILRIIKHVAPDGSVSLDREDIFQG
                     KRKQVFVPFTAKGGGTHSAPEGVSP"
     gene            907..3498
                     /locus_tag="VFH83_11605"
     CDS             907..3498
                     /locus_tag="VFH83_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487060.1"
                     /translation="MARSMRLSDTRMAALFLVSVVVLAFEILVMRVFSVASWSNFGSM
                     VISIALLGFGVAGTLITILGKRIRANPDGWLSVSAYALGPSIALAYTVAQRIPFNPVL
                     IVTDPSQLWWIAAYYLLYGLPFFVGALFIGTIFSSLSSQMHTLYFWNMLGSGLGGLIV
                     LGLMFLLPTHYLIYPVLGASLLAALLCTVRWSAGESRFRIRGMEVILSLALALVSVVL
                     PAQFGALNVSDFKPESYARNFPDSKIVYENFSPRGEMRVFESSFFHFAPGLSDNAGAT
                     LAHMPKNAFEGLYIDGEGPVGVMRKLSPEEEAYIDFLPMSAPYLLLSRPKVLELRLGG
                     SAGVQTALHNGASQVWVVESNPDLVHMLRDVPYFQSYTGRVLQDPRVRLVNNEVRAFA
                     GSTRDRFDLVEIGLVDSIGLSQAGGYSVEENYLYTEEAIREYLRCLAPGGVLSITVWD
                     RLSPPRNVPKLMTTIVEAMRQEGITRPDQRIFAFNLLLSTATVLVKNGDFTPSETETL
                     AAYCKRMSFDADYYPGIGPAPVSFATLMDGYAALYADPNREGSAAGSVPLRPNDLYRL
                     ALHQLLFGSPSELYDRYVFDIRPATDDKPYYSGYLKLPSIPMFLRQLGDISEEWGYLL
                     LLATLLQSLIFGALVVLLPMASSWRELFRGRQGTFGIIVYYGFLGLAYMMAEIYLIQR
                     FVYYLADPVYVNSLVIALLLIASGIGSLVSTSIRGRKTTIVLLATVGIAACAAFYAFG
                     MSPILRATLGLPLAVKALVAAACIAPLGLLLGVPFPTGLASLSASRRGVLPWAWGVNG
                     ALSVTGSVLTRIVSTSLGFRTVLACMAVLYLGAGLLFRVNERGKAEEAVGLKERAGGI
                     RERAGGT"
BASE COUNT          599 a         1192 c         1166 g          669 t
ORIGIN      
        1 ggtgccggcc cggcagaccc cgggcgaggc cctggggaag agcccccagg ggagctccga
       61 ggccgggccc gtggcctccg aaaccggcat cgccctgccc tccactcccg gcgaaaccta
      121 cgacatcacg aagcgagtcg actggccgcc cacgaccggc cgtaaggcgt acctgagctg
      181 gatgcttgcc catactgccg agacggaaaa gttcctgaac gccaagtggg accgagccca
      241 gacaatcctg gccatcggga acatcacgca caagcgggtc ctggaagcct tcctgacgac
      301 accgcgggag gagttcagcc gagaccccaa gcgggcatac gacaacgcgg cccttcccat
      361 cggttggggg cagacgatat cggggccgca catggtctcc cgaatgaccg actacctgga
      421 tccccagccg gaccagaagg tgctggagat cgggacgggc tcagggtacc agtccgcggt
      481 cctctcggag ctctccaacc acgtgtacac gatcgagatc gtcgagccac tggcgcagaa
      541 gaccaacgcg atctaccagt cgctggagga tcggtatccc gagtaccgca acatccagcg
      601 caaggtcgat gacgggtact acggctggcc tcagaatgct cccttcgacc ggatcatcgt
      661 gacctgcggg atcgaccacg tcccgcccga gctgcttcga gagctggcgc cagaggggat
      721 catggtgatc cccatcgggc cgccctccgg ccagaccatc ctccgcatca tcaagcacgt
      781 ggccccggac ggcagcgtga gccttgaccg cgaggacatc ttccaaggca agcggaagca
      841 ggtgttcgtg cccttcacgg ccaaaggtgg cggaacgcat agcgcacccg aaggagtgag
      901 cccctgatgg cgcgctccat gcgtttgtcg gacacgcgga tggccgcgct cttcctcgta
      961 tccgttgtcg tgctggcgtt cgagatcctg gtgatgcggg tcttctcggt ggccagctgg
     1021 tccaacttcg ggtccatggt catcagcata gccctcctgg ggttcggggt cgcggggacg
     1081 ctgatcacga tcctgggtaa gcgtatccgc gcgaacccgg acgggtggct gtctgtttcc
     1141 gcctacgccc tgggcccctc cattgcgctg gcgtacacgg tcgcgcagcg catcccgttc
     1201 aaccccgtgc tcatcgtcac ggatccctcg cagctatggt ggatcgcggc gtactacctg
     1261 ctgtacggcc ttccgttctt cgtcggcgcc ctctttatcg ggaccatctt cagctccctg
     1321 agcagccaga tgcacaccct gtacttctgg aacatgctgg ggtccgggct gggtgggttg
     1381 atcgtcctgg ggctcatgtt ccttctgccg acccattacc tcatctaccc ggtcctgggt
     1441 gcgtcccttc tggccgcgct tctgtgtacg gtgcgctgga gcgccggtga gagtcgattc
     1501 cggattcgcg ggatggaggt catcctgagc ctggcgttgg ccctggtcag cgtcgtgctc
     1561 ccagcccagt tcggcgcgtt gaacgtctca gatttcaagc ccgaaagcta cgccagaaac
     1621 ttcccggatt cgaagatcgt gtacgagaac ttcagccccc gtggggagat gagggtgttc
     1681 gagagctcct tcttccactt cgcgccgggc ctgagcgaca acgcaggggc taccctggcg
     1741 cacatgccca agaacgcgtt cgaggggctg tacatcgacg gcgaaggccc ggtcggcgtg
     1801 atgaggaagc taagccccga ggaagaggcg tatatcgact ttctcccgat gtccgcgccc
     1861 tacctcctct tgagccggcc gaaggtcctc gagctgcgac tgggcggcag cgccggggtg
     1921 cagacagcgc tccacaacgg cgccagccag gtctgggtgg tggagtccaa cccggacctg
     1981 gtccacatgc tgagggacgt cccctacttc cagagctata ccggccgcgt cctccaggac
     2041 ccccgagtgc gcctggtcaa caacgaggtc cgcgcctttg ccgggtccac ccgggatcgg
     2101 ttcgacctgg tggagatcgg cctggtcgac tccattggtc tctctcaggc aggcgggtac
     2161 tcggtggagg agaactacct ctacaccgaa gaggccattc gggagtacct gcggtgcctg
     2221 gccccgggag gcgtcctttc catcaccgtg tgggaccgcc tcagcccccc acggaacgtg
     2281 ccgaagctca tgaccacgat cgtcgaggcg atgcggcagg aggggatcac gaggccggac
     2341 cagcggatct tcgcgttcaa cctgctcctg tcgacggcca cggtcctcgt gaagaacggc
     2401 gatttcaccc cgtcggaaac ggagactctg gctgcctact gcaagcggat gtccttcgac
     2461 gccgactact atccggggat cgggcccgcg cccgtcagct tcgcaacgct gatggacggc
     2521 tacgccgcgc tatacgctga cccgaaccgc gagggctcgg cggccggctc cgtccccctg
     2581 aggcccaacg acctctaccg tctggcgctc caccagcttc tgttcggcag cccctcggag
     2641 ctctacgacc gctacgtgtt cgatatccga cccgccacgg acgacaagcc gtactactcg
     2701 ggctatctga agctgccgtc gatcccgatg ttcctgcggc aactggggga catctccgag
     2761 gagtggggct atctccttct cctggcaacg ctgctgcagt ccctcatctt tggcgccctc
     2821 gtcgtcctcc tgcccatggc cagtagctgg cgcgagctct tccgggggag gcagggcacc
     2881 ttcgggatca tcgtctatta cgggtttctg ggcctggcct acatgatggc ggagatctac
     2941 ctgatccagc gtttcgtcta ctacctggcc gacccggtgt acgtgaactc cttggtgatc
     3001 gccctgctcc tgatcgcttc cggcatcggc agcctggtct ccacaagcat tcggggccgc
     3061 aagaccacga tcgtgctgtt ggcaaccgtc ggcatcgccg cttgcgccgc gttctatgcc
     3121 ttcgggatgt cgcccatcct gagggccacc cttggcctgc ccctggcggt aaaggccttg
     3181 gttgccgcgg cctgcatagc gcccctcggg ttgctcctgg gggtcccgtt ccctacgggc
     3241 ctggcgtccc tgtcggccag ccgacgcggg gtgctgccgt gggcctgggg cgtcaacggc
     3301 gcgttgtccg tgacgggctc ggtactcacc aggattgtgt ccacgtccct cgggtttcgg
     3361 accgtgctcg cgtgcatggc ggtcctgtac ctgggcgcgg ggcttctgtt tcgggtgaat
     3421 gagagaggga aggcggaaga ggcagtgggg ttgaaagagc gcgcgggcgg gatcagagag
     3481 cgcgcaggcg ggacgtgagg cggggaaggg cggcggggac gagggaacgg gcgggagaaa
     3541 gcctgaagac tgcggctaag ctttttcagc ccgaatgcag agacagccgt ctgaagcgcc
     3601 ggcagcgaag cttcgatgcc ggtgct
//
LOCUS       DASRZS010000476         5306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1416448, whole genome shotgun sequence.
ACCESSION   DASRZS010000476 DASRZS010000000
VERSION     DASRZS010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416448"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFH83_11610"
     CDS             complement(<1..319)
                     /locus_tag="VFH83_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6487061.1"
                     /translation="MPAAARREPLAGVFSPRRPLGGTGFRVTGLGIGDVADRTVPLEQ
                     CVATVRRALDAGLNLVDTAPGYEQGYSEQIVGQALKGRRDGIFVVDKIDNHDQPVLPQ
                     IEES"
     gene            complement(328..855)
                     /locus_tag="VFH83_11615"
     CDS             complement(328..855)
                     /locus_tag="VFH83_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487062.1"
                     /translation="MAATVLDVNRAPVLTLWAAVVAERQGYDWDAALTLGKVMAGLNA
                     QAKGRTLGIYTPKAASAEDRARRPKDQLWVTVCGRPVPALKTPQGIRALVKEATVAPE
                     SVQRYLLGAFGESLPEVRKAMEDLAASVAPTEIEQRAFGLYEQFRPQIASGTRGWGQK
                     GKLDLDKIRSLAGTR"
     gene            complement(869..2071)
                     /locus_tag="VFH83_11620"
     CDS             complement(869..2071)
                     /locus_tag="VFH83_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HET6487063.1"
                     /translation="MDTTGAGVLGAARALRDSSAQILSSIVRIPSFSGKEEAVCRAIA
                     DLCREARFDEVRFDGLGSVVARVGHGPRTLAIDAHVDTVGVGDRTRWEEDPFSGIIRD
                     GLVLGRGTSDQKGGAAAMITAGRMLKESGYDGVFSVLFAFTVLEEDCDGLCWRYLIER
                     EGIRPELAVSTEPTSCRLYRGQRGRMEIQASVRGVSAHGSAPERGQSAAYKAARAALS
                     IERLNQELPSDDFLGKGTVVVSRVEAHGPSQCAVPDLGSLYLDRRLTAGETSEKALAE
                     VRRAMGKDAEDVALPRYEGTGWTGERYSQDLYFPTWTVPLEHPLVTGGSRAYRALFGR
                     QPEIGRWTFSTNGVAICGYHGIPCIGFGPGDEEQAHAPNERTRIDDLVTAAAFYAMLP
                     IALEIKAD"
     gene            complement(2240..2743)
                     /locus_tag="VFH83_11625"
     CDS             complement(2240..2743)
                     /locus_tag="VFH83_11625"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET6487064.1"
                     /translation="MESRDYQITITVNIPAEEAFRKIGEVSLWWTKGIRGQAKLLGDR
                     FTMRVGESETYVDLEVVELEPNARIVWRVTDCNLHWITNRAEWTGTRILWEVTSKNGG
                     TEIRMTHIGLIPGAECYDVCKPGWDFHVGKSLYRFLAENIELTAESEAANREARRDIA
                     APSSRPR"
     gene            complement(2798..3229)
                     /locus_tag="VFH83_11630"
     CDS             complement(2798..3229)
                     /locus_tag="VFH83_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET6487065.1"
                     /translation="MRSGMQTYEFTLERVIPAPVDEVFDSWLDPGIPGKPWHDADKLI
                     FDPKVDGLFYVLMQVPHYGRFIRIDRPDAIQHTFVSPNTRGLESLVTVTFQRKGEDTL
                     MRLRHEGLPDDEGGRGHERGWTFFLGLLVEHFSRRRRSGGS"
     gene            complement(3226..3555)
                     /locus_tag="VFH83_11635"
     CDS             complement(3226..3555)
                     /locus_tag="VFH83_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6487066.1"
                     /translation="MDQLTDVLTALSHPSRRAIIRQLASSGPTRFTDVADPLEVTLNA
                     VTKHLKLLDRAGLILRERQGREVFISLRPEPLRLVAGWVHDYERYWSEQLDRFEGYFK
                     ERKRRRS"
     gene            3931..4074
                     /locus_tag="VFH83_11640"
                     /pseudo
     CDS             3931..4074
                     /locus_tag="VFH83_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000182.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
     gene            complement(4362..4826)
                     /locus_tag="VFH83_11645"
     CDS             complement(4362..4826)
                     /locus_tag="VFH83_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487067.1"
                     /translation="MNSNENGRGRALTALVLTNVVLLVAQYALGMYVNLFVQFPDSLP
                     QGNAWAWVFSNSSVTVAHVVLGSAIVLLSVVVLGLSIAHRRALVITSSALGLLMVLFA
                     WLSGSDFLSRGQQDSSSYTMAMGFVAAVVYYVWQFILLFRQKSASRKQAGER"
     gene            complement(4862..>5306)
                     /locus_tag="VFH83_11650"
     CDS             complement(4862..>5306)
                     /locus_tag="VFH83_11650"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET6487068.1"
                     /translation="RIRTFTMDRDSRINGQKMNPAVVNQIVFKDDVEIWQIVNQSPNY
                     HTFHVHDVQFLIVDRNGKAPEPSDQGWEDNVLLYPGDHVRILVQFKYYTDPHHPYMFH
                     CHILEHEDMGMMGQFVVVEKGTKNSQVSVDPAYINVFSHNTDMRP"
BASE COUNT          976 a         1724 c         1601 g         1005 t
ORIGIN      
        1 ggctctcctc gatctgcgga agcaccggct ggtcgtggtt atcgatcttg tccaccacga
       61 agatgccgtc ccgccgaccc ttgagcgcct gacccacgat ctgctcgctg tagccctgct
      121 cgtagccggg cgcggtatcc accaggttca gaccggcgtc cagcgcgcgg cgaaccgtcg
      181 caacgcactg ctccagaggc acggtgcggt ccgccacgtc tccgatcccc aggccggtca
      241 cgcggaaacc cgttcctccc aagggccggc gaggcgagaa gacaccggcg agcggttcac
      301 ggcgcgctgc ggctggcatg ggtcctccta tcgggtcccg gccagggacc ggatcttatc
      361 cagatccagc ttccccttct gcccccagcc gcgcgtgccg gaagcgatct ggggacggaa
      421 ctgctcgtaa aggccaaacg ccctctgctc gatttcggtc ggcgcaacgg aggctgcgag
      481 gtcctccatg gccttgcgga cctctggcag ggattcgcca aaggccccca gcaagtagcg
      541 ctggacggac tccggggcga cggtagcttc cttgacgagc gctcggattc cctggggcgt
      601 cttcagcgcc ggaacgggtc gaccgcacac cgtgacccag agctggtcct tcggccggcg
      661 cgccctgtcc tccgcgctgg ccgcctttgg cgtgtagatg cctagcgtgc ggcccttggc
      721 ctgggcgttg agcccggcca tgaccttccc cagggtgaga gcggcgtccc agtcgtagcc
      781 ttgacgttcc gccaccacgg ccgcccagag cgtgaggacc ggcgcccggt tcacatccaa
      841 gacggttgct gccatggctg cctccccctc aatctgcttt gatctccagc gcgatcggga
      901 gcatcgcgta gaacgccgcg gcggtgacca ggtcatcgat ccgggtgcgc tcattgggag
      961 cgtgcgcctg ctcctcatct cccggtccga agccgatgca ggggatcccg tggtagccgc
     1021 agatcgccac accgttggtg ctgaacgtcc atcggccgat ctcgggctgc cggccgaaga
     1081 gcgctcgata ggcgcggctc cccccggtca ccagcggatg ctccaggggc accgtccagg
     1141 tggggaagta aaggtcctgg ctgtatcgct ctcccgtcca ccccgtgcct tcataccggg
     1201 gcaaggccac gtcctctgca tccttaccca tcgctcgccg cacttccgcc aaagccttct
     1261 ccgaagtctc cccggcggtc aggcggcgat ccaggtagag ggagcccaga tccgggaccg
     1321 cgcactggct cggtccgtgc gcctcgactc gagagaccac gacggtgccc ttgcccagga
     1381 agtcatcgga cggaagctcc tggttcagcc tctcgatgga taaggcggct cgagccgcct
     1441 tgtatgccgc gctctgtccg cgctccggcg cgctcccgtg cgcggagact ccgcggacgg
     1501 atgcctggat ctccatgcgg ccgcgctggc ctcgatagag ccggcaggaa gtcggctccg
     1561 tggagacggc gagctcgggg cggatcccct ctctctcgat gaggtagcgc cagcacaaac
     1621 cgtcgcagtc ttcctccagg accgtgaacg cgaagagcac gctgaagaca ccgtcgtagc
     1681 cggactcctt gagcatccgg cccgcggtga tcatggccgc ggccccgccc ttctggtccg
     1741 aagtgcctcg gcccaaaacg agcccgtcac ggatgattcc ggagaacggg tcctcttccc
     1801 agcgcgtccg gtcccctacc cccaccgtgt ccacgtgagc gtcgatggcg agcgtgcgcg
     1861 ggccatggcc cacgcgtgca acgacgctgc ccaggccgtc gaaccggacc tcgtcgaagc
     1921 gcgcttcgcg gcagaggtcg gcgatggcgc ggcagaccgc ctcctccttg ccgctgaagg
     1981 aagggatccg tacgatggag gaaaggatct gtgcggagga gtcacgcagg gctcgagcag
     2041 ccccgagcac gccggcgcca gtggtgtcca tgaggccatc agtatggacc caaaggccgc
     2101 cggctcgcaa gccgcgggat cagctcaaga gacccggatg cagacggccc gtgctcaaga
     2161 ctccgcgccc ttgaggagtt tccgcagctc cgggatcagc tcccgggtgg cagagaccgc
     2221 cccggaccaa catcgcgtcc taccgcggac gtgaggacgg agcggcaatg tcacgccttg
     2281 cctcccggtt cgccgcctcg gactcggccg taagctcgat gttctcggcc aggaaccggt
     2341 aaaggctctt gccaacgtgg aaatcccatc cgggcttgca gacatcgtag cactcggcac
     2401 cgggaatcag gccaatatgg gtcattctga tctcggtccc cccgttcttc gacgtgacct
     2461 cccagaggat cctggtgcct gtccactcgg ccctgttggt tatccagtgc agattgcagt
     2521 cggtgactct ccagacgatt cttgcgtttg gctcgagctc cacgacctca agatcgacat
     2581 aggtttcact ttcaccaacg cgcatggtga agcgatctcc cagcagcttc gcctggcctc
     2641 tgattccttt cgtccaccag agcgatactt caccgatctt tcgaaaagcc tcttcggctg
     2701 ggatgttcac cgtgatggtg atctgataat cccgagactc catatacgtc tcctctcgtt
     2761 gttggtcgat gctccgcttc gggagctcgt ttccgtgtca tgaaccaccg cttcgacgcc
     2821 tgcgcgagaa gtgctccacc aggagcccca gaaaaaaggt ccagccccgc tcatgtcccc
     2881 tgcctccctc atcatccgga agaccctcgt ggcgaagcct catcagggta tcttccccct
     2941 tccgctggaa ggtgaccgtg acaagggact cgaggccccg ggtattggga gagacgaacg
     3001 tgtgctggat cgcgtcgggt cgatcgatcc ttatgaagcg tccgtagtgg ggtacctgca
     3061 tcagcacgta gaacaggccg tcgacttttg gatcgaagat caacttgtcg gcgtcgtgcc
     3121 agggcttccc cggaatcccg ggatccagcc aggagtcaaa gacctcgtcc accggagcgg
     3181 ggatcacgcg ctcaagggtg aactcatatg tctgcattcc agacctcatg accgcctcct
     3241 ctttctctcc ttgaaatacc cctcgaaccg atcgagctgt tcactccagt aacgctcata
     3301 gtcgtgcacc cagccagcca ccaggcgaag cggctcaggg cgaagggaaa tgaagacctc
     3361 ccgcccctgc cgttcgcgca agatcaaccc cgcccggtcc agcagcttga ggtgcttggt
     3421 aaccgcattc agagtcacct cgagcgggtc cgcgacgtcg gtgaaacgcg taggtccaga
     3481 cgacgcgagc tgccggatga ttgccctccg ggaagggtgc gacagtgccg tgaggacatc
     3541 cgtaagctgg tccacgacga tattatacca aaaggtgtaa tattcggcaa gaccaatggg
     3601 ggcatgtgcc tcgcagctcg accccaaggt gcctgccgct gcaaccgcca gatgcgcgtg
     3661 tgtccaacga gccagttcca cggtcaagtc ggaagcctga ccacggccgt tggttcgcac
     3721 gatctggcaa ccgcgtcgct tccagcgcct ctgctggatc aggccagagt tccgtaggtt
     3781 taggcagaca cctttctcgg tcattcttta tattccctcc gtgagctgct gtggttgctg
     3841 gggtttcgtg gatgcatgct ggtccgcaag ctcggacaat gcggcaaggg accaggattc
     3901 ccaagcagat cattcatggg aggattggcc ttgagagacg tagttgtcgt ggtcggaccg
     3961 ggtcagatcg gtcaagcgat cgcccgtcga gtcggcgcag gtgcgcgtat cctgctggca
     4021 gatctgcgtc aggacaacgc caaagccgcg ggggaagtca tgaggaacgc cggataagcg
     4081 gtagtcggtt tgcgggttcg gaggaggccg acagtgttca gacgccccgc aacgtccgcg
     4141 ttgcagcgct cagttgcgaa gaggtccagg gacatcgcgg caagacgccg cgagtccctg
     4201 gcccaagggg gcgaggatca gaaggaagca aagctcatcc cgtagcgaaa cggcggatcg
     4261 caggcggcaa cggcacttcc gattttcccg ccgaaactgg ttgcccagcg cgttgcgctt
     4321 attcccacgg cacccacggg aaggtgccgc tctgggcctc cctatctttc gcccgcctgc
     4381 ttcctcgacg cgctcttctg ccggaacagc aggatgaact gccacacgta gtaaaccact
     4441 gccgccacga atcccatcgc cattgtgtag gagcttgagt cctgctgacc tcgtgagagg
     4501 aagtcagagc cgctcagcca ggcaaacagc accatcagca gacccagcgc ggacgacgtg
     4561 atgacaagcg ctcgccggtg cgcgatggac aagcccagca ctacaacgga aagcagcacg
     4621 atggcgcttc ccaggacaac gtgtgccacg gtgacagagc tgttcgagaa cacccaggcc
     4681 caggcatttc cctgcggcag ggagtccggg aactgaacga acaggttcac gtacatcccc
     4741 aaagcatatt gggcgaccag gagcactacg ttcgtcagaa ccaatgccgt cagggctctc
     4801 cctcttccgt tttcgttcga attcatgtca tcgcctccat tcgaaagtgc taatcgactg
     4861 ctcacggacg catgtcggtg ttatggctga acacattgat gtaggccggg tcgacagaca
     4921 cctggctgtt tttcgtccct ttctcgacga cgacgaactg tcccatcatt cccatgtctt
     4981 cgtgctcgag aatgtggcag tggaacatgt aggggtggtg cggatccgtg tagtacttga
     5041 actggacaag aatgcggacg tgatcacccg ggtagagaag gacgttatct tcccatccct
     5101 ggtcgcttgg ctcaggtgcc ttcccgttgc ggtcaacgat aaggaactgg acgtcatgaa
     5161 catggaatgt atggtagtta gggctttggt tgacgatctg ccagatctcg acgtcgtcct
     5221 tgaacacgat ttggttcacc acggccgggt tcatcttctg gccgtttatc ctcgagtcac
     5281 ggtccatcgt gaatgtgcgg atcctt
//
LOCUS       DASRZS010000477         6506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2951457, whole genome shotgun sequence.
ACCESSION   DASRZS010000477 DASRZS010000000
VERSION     DASRZS010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6506
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2951457"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            398..757
                     /locus_tag="VFH83_11655"
     CDS             398..757
                     /locus_tag="VFH83_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET6487069.1"
                     /translation="MAVLLSDKAAGQFVSFAAEQGSKGAMLRIWVAGLGCSGFRYGMG
                     IEEKAPEEGDQVFESNGVRLVIDAQSLSYMDGATVTWVDDPQNSGFSIENPNPAPERQ
                     CNCGSAGCGVQGESSEA"
     gene            complement(790..1875)
                     /gene="rsgA"
                     /locus_tag="VFH83_11660"
     CDS             complement(790..1875)
                     /gene="rsgA"
                     /locus_tag="VFH83_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET6487070.1"
                     /translation="MQLSSSQLLSIGWNSRLDIQFQPHAQAGLSPARVAAAHRDLCIL
                     LTESGELSAEVSGRFRLQTALTSGFPAAGDWVAASLRPQEGKATIHAVLPRSSAFTRK
                     AAGERVQEQVIAANIDTAIIVMGLDGDYNLKRLERYLTLAWGSGAVPLVVLNKSDLCE
                     DPDGRRLEVEAAAIGVTVLLTAAEPGQGLGELHTRLAPGTTAALLGSSGVGKSTILNR
                     LLGERHMDTAAVREDDSRGRHTTTHRELVVLPSGAMVIDNPGLREVGLWGDEEALEGS
                     FPDVEELARSCRFSDCRHDGEPGCAVRRAVADGSLAPERFESWRKLQRELRFLARRAD
                     ESLRRAQLQRSKSLAKFQKRFRKDARF"
     gene            complement(2059..3339)
                     /locus_tag="VFH83_11665"
     CDS             complement(2059..3339)
                     /locus_tag="VFH83_11665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET6487071.1"
                     /translation="MALWIAFGAAGAWAAPRETARAPASQNFGLSARAAIMIDAATGR
                     VLYEHDADQPFLPASLTKLMSLHIVYEKLKDRTIGRDDVVYLTRNAWANNQAPGSTLM
                     HLGPGQIVTVEELMKGAAISSGNDAAVALAEYVAGSTDKFVQLMNEESRFMGYRVTHY
                     EDPAGVGPDNRVTAREFADFCRRYIELHPEALGELHSLREFDYPLARNMPDGRLPPRG
                     TRRTYNHNGLVWDNLGVDGLKTGRFDGENFTAAITAQRGETRLIVVLLGVPGRSLIEG
                     ARNRSDDGATLLTYGFNTYDTMELEPPRLGQIGVWRGVARKLGVVAGAPVRLTLSDSE
                     LSNLQGVLEVQGPIVAPISRGEKVGEIVYSALGGEVGRIPVVADEDVAGAGPIRQAMD
                     WLVMGVSSLVGSPPRGERTVLDVGNSARLLSDRY"
     gene            complement(3446..3985)
                     /locus_tag="VFH83_11670"
     CDS             complement(3446..3985)
                     /locus_tag="VFH83_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6487072.1"
                     /translation="MTNRVFTFDLGRIMDEAFKFAENFGDSFGPEAEEKLRQAAEQFG
                     RGPFGNPDYYPAYLYPPATIYLTKDKRLVLEMALAGFSESDLTVQFRGDHLLFSARAP
                     KLPEPEEGVQYFKRRLKLKDIEEQRYYVPADKFDQAGTEASFHSGMLRLVVPPRLQTS
                     TGDEIRIQIKSAGEPASGA"
     gene            complement(4150..6159)
                     /locus_tag="VFH83_11675"
     CDS             complement(4150..6159)
                     /locus_tag="VFH83_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006367211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6487073.1"
                     /translation="MSAHKPEPLLDKLRTAVLGEARDLKDPSLFHKLSLVAFFAWVGL
                     GADGLSSSNYGPEESFLALGAHTHLGIFVALATALTIFVIASSYSQIVELFPGGGGGY
                     LVASKLLSPTLGMISGCALLIDYVLTITLSVASGADAIFSFLPVAVLRLKLPFALLGV
                     LLLMVLNLRGVRESVLPLVPVFLLFLLTHTFAILFAIGTHVSAAGQIFTDTAAEARSS
                     AGEIGGFALFILVLRAYSMGAGTYTGIEAVSNGLPILREPRVHTARRTMRYMAASLAF
                     MAAGLMIAYLLFRVGPVRGKTLNAVLMGSIAAGWGTPGAVFVLVTLISEAVLLFVAAQ
                     TGFLDGPRVLANMSLDRWFPSQFGLLSERLVIKNGIVIMGVAAIVLMLVSRGSVQFLV
                     VLYAINVFITFCLSQAGMVRHWWRERGKVPRWKRKILINGIGLLLTGFILISQLILKF
                     GQGGWITLLVTGSLVILALTINRFYRGTGKRLTRLDRLMTAAAEAEEASAAALSKPGR
                     KRPAPTYDPNGKTAVLLVSGFNGTGLHTLVNVRGVFGDSFRNWFFIEAGIVDADRFKG
                     AEAIESLRHRVDQDLVRYVKFLNTEGFYAQGFPTIGTDIVDEICTLAERIHRKHPKAV
                     FFGGQIVFREDTFLYRLLYNHTAFAVQRRLHQLGIPFFVMPVRVT"
     gene            complement(6242..>6506)
                     /locus_tag="VFH83_11680"
     CDS             complement(6242..>6506)
                     /locus_tag="VFH83_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HET6487074.1"
                     /translation="SLEAFLKKDVEAARAIPKEDSQVDALYNQIYRELITYIIADPKA
                     IDQATRLTWVAHNLERVADRVGNICERIVFSITGHGEELDTPP"
BASE COUNT         1165 a         2100 c         2122 g         1119 t
ORIGIN      
        1 attgagcctg ttcgtggcgt ccgtctcagc gcgcatctcc cagcgccgct ctggattcct
       61 acgaaaatgc accgtgcgat tctgtagatt ccgcgagggt ggggaacaag gtccagcgcc
      121 ctgctctgga tttctacgaa aatgcacggt gcgattctgt agattccgcg agggcgggga
      181 acaaggtcca acgcccggct ctggattcct acgaaaatgc acggtgcgat tctgtagatt
      241 ccgcgagggc ggggaaccag gtccaacgcc cggctctgga ttcctacgaa aacgcacggt
      301 gcgattctgt agattccgcg agggcggcaa tgggtgccgg gaaagcgctc aggcggctgt
      361 tgactcctga cacccttttt agtcatagtc taggggtatg gcagtgttgc tttccgacaa
      421 ggccgcgggc cagttcgttt catttgcggc ggagcaggga tccaaggggg ccatgctgcg
      481 gatctgggtt gcgggactgg gatgctccgg gttccgctac ggcatgggaa tcgaagagaa
      541 ggcgcctgaa gagggcgacc aggtattcga gtccaacggc gttcggctgg tgatcgacgc
      601 ccagagcctg agctatatgg atggggcgac cgtcacctgg gtcgacgatc cccagaacag
      661 cggcttcagc atcgagaacc ccaacccggc ccctgagcgg cagtgcaact gcggctccgc
      721 gggctgtggt gtccagggag agagctccga ggcataacac cgcggagccc tccgtggtct
      781 atctgcgcgt cagaaccgcg catccttccg aaaacgcttc tgaaacttcg cgaggctttt
      841 gctgcgttgt agctgcgccc gccgcaggct ctcgtctgca cgtcgggcga ggaaccgcag
      901 ctcacgctga agtttgcgcc agctttcgaa acgctccggc gcaaggctgc catccgcgac
      961 cgcccgccgc accgcgcagc cgggttctcc gtcgtgccgg caatcggaga aacggcagga
     1021 gcgggcaagc tcctccacgt ccggaaacga cccttccagg gcctcctcat cgccccagag
     1081 acctacctct cgtaagcccg gattgtcgat gaccatggcg ccggagggca ggaccaccag
     1141 ctcgcggtgg gtggtggtgt gtcttccgcg gctgtcgtct tcccgcaccg ccgcagtgtc
     1201 catgtgtcgc tccccgagaa ggcggttgag gatggtggac ttgcctacgc ccgatgagcc
     1261 aagcagcgcc gcggtggtcc ccggcgcaag ccgtgtatgc agctccccga gtccctgacc
     1321 aggctcggcc gcggtcagga ggaccgttac gcctatggcc gcggcttcca cctcgaggcg
     1381 acgaccgtcc gggtcctcac agaggtccga cttgttcagg acgacgagag gcaccgcgcc
     1441 gcttccccag gcgagggtga gataccgttc caggcgcttg aggttgtagt ctccgtccag
     1501 gcccatgacg atgatggcag tgtcgatgtt ggccgcgatc acctgctcct gcacgcgttc
     1561 tcctgccgcc ttccgcgtga aggcgctgga acgcgggagg acggcgtgga tggtagcctt
     1621 gccttcctga ggacggagtg acgcggccac ccagtctccg gctgccggga accctgaggt
     1681 cagcgcggtc tgcagacgaa atctgccgga gacctccgcc gaaagctccc cggactcggt
     1741 cagaaggatg cacaggtctc gatgcgcggc ggcaacgcgc gccggtgaca gacctgcctg
     1801 ggcatgaggt tgaaactgga tatcgagtcg gctgttccag ccgatcgaca aaagctgcga
     1861 cgatgaaagc tgcaatggga tccctccctg cacgacgccc cgaagggctt acgcggctcg
     1921 gcgaggaatc agactggaag gagaagttac ctcggcgagc ccctgaggac agagttgttg
     1981 cggggaacgc ttcgtaccga cttcatctct ctaccctcct ttcgccgtca ggctcgccaa
     2041 acggcattcg attctggatc aatagcggtc ggatagtagc cgcgcggagt tgcccacgtc
     2101 aagtaccgtt ctctctcctc tgggaggcga cccaacgagg gaagagacac ccatgacgag
     2161 ccaatccatg gcctggcgga tcggtccggc gccggcaaca tcctcatccg cgaccacggg
     2221 gatgcgcccc acctccccac ccaacgcgga gtagacgatc tctccgacct tctccccacg
     2281 ggagatcggc gccacgatag ggccctgtac ctccagaacg ccctggaggt tggaaagctc
     2341 gctgtcgctc agggtgagcc gcacgggcgc gcccgccacc acgcccagct tccgcgcgac
     2401 gcctctccac acgccgatct gccctaaccg cgggggctcc agctccatgg tgtcgtacgt
     2461 gttgaacccg tacgtcagga gcgtggcgcc gtcatccgat cggtttcggg ccccttcgat
     2521 gagggaccgg cccggcaccc ccaggaggac cacgatcagc ctcgtctcac cgcgctgcgc
     2581 cgtgatggct gccgtgaagt tctctccgtc gaaccggccc gtcttcaggc cgtccacgcc
     2641 caggttgtcc cacaccaggc cgttgtggtt atacgtcctg cgcgtgccgc ggggaggcag
     2701 cctgccgtcc ggcatgttgc gagccaacgg gtagtcgaac tcccgcaggc tgtgcagctc
     2761 tccgagggct tccgggtgca gctcgatgta gcgccggcag aagtccgcga actcccgcgc
     2821 ggtcacccgg ttatccggcc cgacgcccgc ggggtcttcg tagtgggtca cccggtagcc
     2881 catgaatcgg ctctcctcgt tcatgagctg gacgaacttg tccgtgcttc cggcaacgta
     2941 ctccgccagc gcgaccgcgg cgtcgttccc ggaggagatg gcggccccct tcatgagctc
     3001 ttccacggtc acgatctgtc cgggaccgag gtgcatgagc gtggaacccg gcgcctggtt
     3061 gttggcccac gcgttacggg taaggtacac cacgtcatcc cggccgatgg tccggtcctt
     3121 cagcttctca tagacgatgt gcagggacat cagcttggtg agggacgcgg ggaggaacgg
     3181 ctggtccgcg tcatgctcgt acagcacgcg tccggtggcc gcgtcgatca tgatggccgc
     3241 gcgggcgctc agaccgaagt tctgcgacgc cggtgcccgc gcggtctctc ggggggctgc
     3301 ccacgccccc gctgcgccga aggcaatcca aagcgccacg gcgcacacgt agagcggagc
     3361 gcggcggccc gcgcggttgg aggattgagg tggccaatgt cccagcccag cgcccgccag
     3421 ccggcctccc tgcgggcgcc ccgtctcagg ctccagaggc tggctctccc gctgacttga
     3481 tttgaatgcg aatttcatcc cccgtgcttg tctgcagccg aggcggcacc acgaggcgaa
     3541 gcatcccgct atggaagctc gcttccgtgc cagcctggtc gaacttgtcc gcgggcacgt
     3601 agtagcgctg ctcctcgatg tccttgagct tgagccggcg cttgaagtac tgcacgcctt
     3661 cctcgggctc gggcagcttc ggcgcccgcg ccgagaacaa caggtggtct ccccggaact
     3721 ggacggtcag gtccgactcg ctgaagcctg ccagggccat ctccagcacc aggcgcttgt
     3781 ccttggtcag gtagatcgtg gcgggcgggt agaggtaggc ggggtagtag tccggattcc
     3841 cgaacgggcc tcgtccaaac tgctcggcgg cctgccggag cttctcctcc gcctccgggc
     3901 cgaaggagtc cccgaagttc tccgcgaact tgaacgcctc atccatgatc cggccgagat
     3961 cgaacgtgaa aaccctgttt gtcatgacgg cacctccgtg cggctggtga cattcgcctc
     4021 cctctcgggc aggcgctgcg gtccccgtac gggcaaccgc ggaaggcctt tggccttctt
     4081 gaggtcaatg tacagaggtt ggccggctcg gacaagggct ccgagtcaag agctccgaag
     4141 gcatcggcat cacgtgaccc gcaccggcat cacgaagaac gggataccca actggtgcaa
     4201 ccttcgctgg acggcaaagg cggtgtggtt gtagagcagc cggtacagga acgtatcctc
     4261 gcggaagacg atctggcctc cgaaaaagac ggccttgggg tgcttgcgat ggattctctc
     4321 cgccagggtg cagatctcat cgacgatgtc cgtcccgatc gtggggaacc cctgggcgta
     4381 gaacccctcg gtattcagga atttcacgta gcggacgagg tcctggtcca cacgatggcg
     4441 caggctctcg atggcctccg cgcccttgaa ccggtccgcg tctacgatgc cggcctcgat
     4501 gaagaaccaa ttccggaacg agtccccgaa cacccctcgg acgttcacca gcgtgtgcaa
     4561 ccccgtgccg ttgaaaccgg aaaccaggag gacggcggtc ttcccgttcg ggtcataggt
     4621 gggggcgggc cgctttcgcc ccggcttgct gagggccgcg gccgacgcct cctccgcctc
     4681 ggccgcggcc gtcatcaggc ggtccaggcg ggtcagccgc tttcccgttc cccggtagaa
     4741 ccggttgatc gtgagagcca ggatcaccag ggagccggtg accaggagcg tgatccaccc
     4801 gccctgcccg aacttgagga tgagctgaga gatcaggatg aaccccgtca ggaggaggcc
     4861 gatcccgttg atcaggatct tgcgcttcca tcgggggacc ttgcctcgct cccgccacca
     4921 gtggcggacc atgcccgcct gagaaaggca gaaggtgatg aacacgttga tcgcgtagag
     4981 gaccaccagg aactggaccg acccgcggct gaccagcatc agcacgatgg cggccacacc
     5041 catgatgacg atgccgtttt tgatcaccag ccgctcgctc aggaggccga actggctggg
     5101 gaaccatcgg tccagggaca tgttggccag gacccgggga ccgtcgagaa accccgtctg
     5161 ggcagccacg aacaggagga ccgcctcgga gatgagcgtc accagcacga ataccgcccc
     5221 gggggtcccc cagcccgcgg caatgctgcc catgagcacg gcgttgagcg tcttgccccg
     5281 caccggtccc acccggaaga gaaggtacgc gatcatcagc ccggccgcca tgaaggcaag
     5341 ggacgccgcc atataccgca tggtgcgccg agccgtgtgg acgcgcggtt cgcgcaggat
     5401 gggcaggccg ttgcttaccg cttcgatgcc ggtataggtt ccagcgccca tgctgtaggc
     5461 tcgcaacacc aggatgaaga gggcgaagcc tccgatctcc cccgcggaag agcgcgcctc
     5521 cgccgcggta tccgtgaaga tctgcccggc ggccgacacg tgggtgccga tggcgaaaag
     5581 gatggcgaag gtgtgggtca ggagaaacag caggaacacc ggcaccagcg gcaggaccga
     5641 ctcgcggacg cctcggaggt tgaggaccat caggagcagc actcccagga gcgcgaaggg
     5701 aagcttgagc ctcaacaccg cgacgggaag gaagctgaag atggcgtcgg ccccgctggc
     5761 aacggagagc gtgatggtga gcacgtagtc gatcaggagc gcgcaccctg agatcattcc
     5821 caaggtaggg gacaggagct tactggccac caggtagcca ccgccgcccc ctgggaatag
     5881 ctccacgatc tgtgaataac tcgacgcgat gacaaagatc gtgagtgccg tggccagcgc
     5941 cacgaagatc cccaggtggg tgtgggcgcc cagggccagg aaggattcct ccggtccgta
     6001 gttggaggag gaaagaccgt ccgctcccag ccccacccag gcgaagaagg ccacaaggga
     6061 gagcttgtgg aacagggacg ggtccttcag gtcgcgggcc tctcccagga ccgcagtgcg
     6121 gagcttatcg agcaggggtt cgggtttgtg ggcgctcatg gtgacagaac cagcaggcta
     6181 tcgccggcgg gtctgtcgtt caaggggccc tcggacgccc cggacgctcg cagagagtcc
     6241 tttacggagg ggtgtccagc tcctctccgt gaccggtgat ggagaacacg atgcgctcgc
     6301 agatgttccc cacccggtcc gccacgcgct ccaggttgtg cgccacccag gtcaggcgcg
     6361 tggcctgatc gatagccttg gggtcggcga tgatgtaggt gatcagctcc cggtagatct
     6421 ggttgtacag ggcgtctacc tggctgtctt ccttggggat ggcgcgcgca gcctcaacgt
     6481 ctttcttgag aaaggcttcc agcgag
//
LOCUS       DASRZS010000478         4698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1033071, whole genome shotgun sequence.
ACCESSION   DASRZS010000478 DASRZS010000000
VERSION     DASRZS010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033071"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..977)
                     /locus_tag="VFH83_11685"
     CDS             complement(9..977)
                     /locus_tag="VFH83_11685"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6487075.1"
                     /translation="MRHRHQVSGGRGPVLRHRQRLDRPGQFDKAAQYYKQALATDPTL
                     AKASYNLALAYAQLNRPDDAIAILTKLLAADPKNTAVMSTLGWTYHQEGKDEDALKRY
                     DDVLAVSPADQDALYNSAIVLWKLDRKPAARERLERLLSYTPEALDALYAAGSLSLAM
                     DDPASAADYLSRYLAKKPEDVDAWYLAAAGAERQQRYSKALDAYDKILTLDSKQSEAS
                     FGEARLLLTVVEDPQKGLDELQQALSTGFHDTDAVKVLLADSTLLERDKVEAALKDKG
                     LLPAPSDAASPGSNAETPGAGPPGAGSSGAGAPGASPPPGRAPTSP"
     gene            1204..4248
                     /locus_tag="VFH83_11690"
     CDS             1204..4248
                     /locus_tag="VFH83_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6487076.1"
                     /translation="MPQTPAERTNLGALSKNGKITILGLLKNFYDEDIFTMLVKEIYD
                     YDRTDTSVSEAAIRASGSLGNETAIKPLYQIIERGSMSQRMEAIRALRSIRAPSTVNQ
                     LIKYFNHFPEENLRTEILRAINTITPTAAAVQQLNTAVYGDPKQSDEAKQIAVEALVE
                     AERYPLLKESLSKASPAVQETAFLKMLQSGSQEVLDFRKESLSPAALGCYLCLYAVKA
                     KTPDTTYILETLQDGHRQTLTSFLLALSKFEGRLRFPTRVFRLLLIVPYSDPETESLV
                     GDFLKKIVSEVKEGAPHLVSEFSVVVSAQLDTVFAKIKKNYVSLQGIGNRDVLLTTIL
                     AKLLERHGTPTVLAETQAFFKEESIRPPPLASLRSLLATAPKEERNQLEACIPIFSIT
                     ERKERLAVLASLSRVDLNRPFLLRRLNRLIRVAGSLNLRSASKKIEEILQFARAERVH
                     YLEETSIVTLCQLLTRSVIERARESLRDPQRSTRALNGYIRGARFIPAKAMAAPLIHV
                     VQQPTLNHTSRALTIDSLEAMDLTGIQKALPPLLKVLDLQGVAEDLKLRIGDLMGRAC
                     DAEIGHPALDLTGHPTPIGRRVAVRILRGLAQRRQGPATEVLTNRLYMLLEDSERSVR
                     VEAILALLAMEDDYAVQIVNDYIQAGDVKMITEILAGITRPVTRDTFSLLLAMLRLDD
                     PKVHSLLGALLPELSQGTFSEDLRQGLLDIMNSVHPPVPASTGAELVSVAAPAEQAST
                     LGQAKVEFKFRRENTQELTVFFIDMAGFTEKSNTIRDEMATLIGVIKAFEDIVSNAVE
                     SNGGTIVKKMGDGILAFFKRPLSAAVAALNVQSAIQAYSSMRVEQEKFRARIGLNTGK
                     VIRKDGDIFGEVVNVASRMQSRATPGEVLLTEATYTEIKDYVDCTQLGRLEVKGIKDG
                     VTAFVLQKVRVDLARLRSGEPVEAGPTGNLRDPSLERLKESYFQPTFAVPAGKTVRAA
                     SQLQQIFSDISRSIEDFSEDPHETFRFKQYLQDQWNALIASL"
     gene            4360..>4698
                     /gene="infC"
                     /locus_tag="VFH83_11695"
     CDS             4360..>4698
                     /gene="infC"
                     /locus_tag="VFH83_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET6487077.1"
                     /translation="MAEKDLRINESIRVREVRLIDAEGNQKGIVPTFEALQMARTAGV
                     DLVEIAPQAQPPVCKLLDYGKYKFELEKKNRESRKHQKQIRIKEIRMQPKIDEHDLEF
                     KTRHVQEFLDG"
BASE COUNT          884 a         1517 c         1499 g          798 t
ORIGIN      
        1 cggctgccct aggggctcgt cggggcacgt ccggggggcg gacttgcccc gggagcgccc
       61 gctcccgagg agccggcacc gggaggtccg gcccccgggg tctcagcgtt ggacccgggc
      121 gatgccgcgt cactcggggc cggcagcagc cccttgtctt tgagggccgc ctctaccttg
      181 tcccgctcca ggagcgtcga gtccgccagc aataccttga cggcgtccgt gtcgtggaac
      241 ccggtgctca gggcctgttg cagctcatcc agccccttct gcggatcctc cactacggtc
      301 aggagcaacc gcgcctcgcc gaaggatgcc tccgattgtt tcgagtccag cgtgaggatc
      361 ttgtcgtacg catccagcgc cttggaatac ctctgctgtc gctcggctcc ggccgcggcc
      421 aggtaccagg cgtccacgtc ctcaggcttc ttcgcgagat atcgggacag gtagtcggcg
      481 gcagaagcag ggtcgtccat cgcaagggac agggacccgg cggcgtacag cgcatccagt
      541 gcctccggag tgtacgagag aagccgctcg agcctttccc tggctgccgg ctttcggtcc
      601 agcttccaga ggacgatggc gctgttgtag agggcatcct ggtctgctgg ggagacggcc
      661 aggacgtcgt cgtagcgctt cagcgcatct tcgtccttgc cctcctggtg ataggtccag
      721 ccaagcgtcg acatcacggc ggtgttcttc ggatcggccg caagaagctt cgtcaggatg
      781 gcgatggcgt catcgggccg gttgagctgc gcgtacgcga gcgccagatt gtagctcgcc
      841 ttggcgaggg tgggatccgt cgccagggcc tgcttgtagt attgggcggc cttgtcgaac
      901 tgccccggtc ggtccaggcg ttggcgatgt cgtagtactg gaccgcgacc tcccgagacc
      961 tggtgtcggt gacgcaaccg cccagcagca cggccgcggc gaggacgggg caggcccacc
     1021 gcgccgccct catgcgcgct ctctcatgtc tccactatag aaggattccg cgggcggcac
     1081 aacggtcccc atcaccggga ggggtcttcg gcggaccgga cctgccgttt ggcccggaaa
     1141 ttgacatggc aaccggcaaa tcggtacgat aaaatgtcta gcagcggaac aaacaggccc
     1201 cagatgccac agacaccggc agaacgaaca aacctcggcg cgctcagcaa gaacgggaag
     1261 atcacgatcc tgggcctgct gaaaaacttc tacgacgaag acatcttcac gatgctcgtc
     1321 aaagagatct acgactacga tcggaccgac accagcgtca gcgaggcggc catccgcgcc
     1381 tcggggtccc tgggcaacga aacggccatc aagccgctgt accagatcat cgagcgcggg
     1441 agcatgtccc agcggatgga ggccatccgg gcgctccgtt ccattcgcgc cccatcgacc
     1501 gtcaaccagc tcatcaagta cttcaaccat tttccggagg agaacctccg gaccgagatc
     1561 ctgcgggcca tcaacaccat tactccgacg gccgccgccg tgcagcagct caatacggcg
     1621 gtctatgggg acccgaagca gagcgacgag gccaagcaga tcgcggtgga agcgctggtg
     1681 gaagccgagc ggtacccgct tctcaaggag tctctgtcca aggcgtcgcc ggcggtgcag
     1741 gagaccgcgt tcctgaagat gctgcagtcc ggctcgcagg aggtgctgga cttccgaaag
     1801 gagtcattgt ctcccgccgc cctgggctgc tatctgtgtc tgtacgcggt caaggcgaaa
     1861 acccccgaca ctacctacat cctggagacg ctccaggatg gacatcgcca gacgctgacc
     1921 tcgttcctcc tggccctgtc gaagttcgaa gggaggctga ggttccccac ccgggtcttc
     1981 cgcctgctcc tgatcgtacc ctactcggat cccgagacgg agagcctggt cggtgacttt
     2041 ctcaagaaga tcgtctccga ggtaaaggag ggggcgcccc atctggtgag cgagttctcg
     2101 gttgtagtct ccgcccagct ggacaccgtg ttcgcgaaga tcaagaagaa ctacgtgtcc
     2161 ctgcagggga tcggcaaccg ggacgtcctg ctgactacga tcctggccaa gctcctggag
     2221 cgccacggga cgcccacggt cctggccgag acgcaagcct tcttcaagga agagagcatc
     2281 cggccgccgc cgttggcttc cctccgatcg ctcctggcca ccgccccgaa ggaggagagg
     2341 aaccagctgg aggcctgcat tccgatcttc tccattaccg agcgcaagga gcggctggcc
     2401 gttctggcgt ctctctcccg ggtggacctg aatcgtccct tcctccttcg caggctgaac
     2461 cgcctgatcc gcgtggccgg gtcactcaac ctgcgcagcg cgtcgaagaa gatcgaggag
     2521 atccttcagt tcgccagggc cgagcgcgtc cactacctgg aagagaccag cattgtcacg
     2581 ctctgtcagc tgctgacccg gtccgtcatc gagcgagcga gagagagcct gagggatccg
     2641 cagcgaagca cgcgggcgct caacgggtac atccgcggcg cgcgcttcat ccccgccaag
     2701 gccatggctg cgccgctcat ccacgtggtt cagcagccca ccctcaacca tacgtcccgg
     2761 gcgctgacca tcgattccct cgaggccatg gacctcacgg ggatacagaa ggcactgccg
     2821 cccctgctga aggtgctcga cctgcagggg gtggcggagg atctgaagct gaggatcggc
     2881 gacctgatgg gtcgcgcctg tgacgcggag atcggccatc cggccctcga tctgaccggg
     2941 caccccacgc ccataggccg gagagtggcg gtgcggatcc tccgcggcct ggcacagcgg
     3001 cgtcagggtc cggccaccga ggtcctgacc aaccggctgt acatgctgct ggaggactcc
     3061 gagcgatccg tgcgcgtgga ggccatcctg gcgctcctgg ccatggagga tgactacgcc
     3121 gtccagatcg tgaacgacta tatccaggcc ggtgacgtga agatgatcac ggagattctg
     3181 gccggcatca cccgtcccgt gacccgggat accttctctc ttctgctggc catgctgcgt
     3241 ctggacgatc ccaaggtcca ctccctcttg ggcgcgctgc ttcccgagct gagccagggg
     3301 acgttctccg aagacctccg ccagggactc ctggacatca tgaactccgt ccaccccccg
     3361 gtcccggcat ccacgggcgc ggagctggtg tccgtggcgg cacccgccga gcaggccagc
     3421 acgctgggtc aggcgaaggt ggagttcaag ttccgtcggg agaacacgca ggagctcacc
     3481 gtcttcttta tcgacatggc cggattcacc gagaaatcca acaccatacg ggatgagatg
     3541 gccacgctga tcggcgtcat caaggcgttc gaagacatcg taagcaatgc ggtggagtcc
     3601 aacggtggaa ccatcgtcaa gaagatgggg gacggcatcc tggcgttctt caagcgtccg
     3661 ctctccgcgg ccgtggcggc gctgaacgtc cagagcgcca tccaggcgta cagctccatg
     3721 cgcgtggagc aggagaagtt ccgcgcccgg atcgggctca acacggggaa ggtgatccgg
     3781 aaggacgggg acatcttcgg cgaggtcgtg aacgtcgcct cccgcatgca gagccgcgcg
     3841 acccccggcg aggtcctcct cacggaggcc acctacaccg agatcaagga ttacgtcgac
     3901 tgcacccagc tcggccgcct tgaggtcaag gggatcaagg atggggtgac cgccttcgtg
     3961 ctccagaagg tccgtgtgga cctggcgcgg ctgcggtccg gcgagcccgt ggaggccggt
     4021 ccgacgggga acctccggga tccgtccctg gaacgcctga aggaatccta cttccagccc
     4081 accttcgccg tgcccgcggg gaaaaccgtg cgcgcggcct cccagctcca gcagatcttc
     4141 tccgacatct cccggtccat cgaggatttc tccgaggacc cccacgagac cttccggttc
     4201 aagcagtatc tccaggacca gtggaacgcg ctgatcgcct cgctgtgaag gagatcagcg
     4261 ccggcgccag cgcgggagcc cggcccggcg gcgccatttg acacagttca atccgcgatg
     4321 gtatagtcta caggccccgg taagcgggaa ggaggtctat tggccgaaaa agatctgcgg
     4381 atcaacgaga gcattcgggt ccgcgaggtg cgactcattg atgcggaggg aaatcaaaag
     4441 ggcatcgtgc cgacgttcga agcgctgcag atggcacgca ctgcgggggt ggatctggtg
     4501 gagatagcgc cgcaggcgca accgcccgtg tgcaagctcc tggattacgg gaagtacaag
     4561 ttcgagctcg agaagaaaaa ccgagagtcc cgaaagcacc agaagcagat ccggatcaag
     4621 gagatccgga tgcagcccaa gattgatgag cacgatctgg agttcaagac ccgccatgtg
     4681 caggagtttc tcgatggg
//
LOCUS       DASRZS010000479         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1796342, whole genome shotgun sequence.
ACCESSION   DASRZS010000479 DASRZS010000000
VERSION     DASRZS010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796342"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFH83_11700"
     CDS             complement(<1..632)
                     /locus_tag="VFH83_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HET6487078.1"
                     /translation="MRNLRLAGKIGVGFGLVIAIAMALGAVAVVSMMGVQGDAGRLSD
                     EIVPQVAVANNIERSALLTMYTMRGYAQSGNPDELAKARQNLTDMEKALTDAERLAVK
                     FPRLVVLKKNSVDATTKVNDYKLLFNQTETTINKIHDLHQTQDTAASSFMNACATYLT
                     RNTANLQAAIARRASAAEQRNDLAEITVIGQIIDLGSELRLANYESQTLG"
     gene            complement(916..2022)
                     /locus_tag="VFH83_11705"
     CDS             complement(916..2022)
                     /locus_tag="VFH83_11705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6487079.1"
                     /translation="MIAVIGAGPAGCYYASLAQGDVHLFEEHDTVGSPVSCTGILTDS
                     VKRVLGEIPESLVVSRIRKFKIVAPSGKSLYVDLDKVNMILDRGAFDRYLLRRALDHG
                     ATLHLGEHFLGFERCAGGFQIRTNRGSYAAEMIVGADGPFSAVARSAGMYDRRQFVMG
                     WQSRCEYDGLEPEVTEIRLALGEFSWIVPESQRIARVGVIGPDAPGLRRDYHRLLGAS
                     KILEDQSGVIPVYNPRQKLRLRGEDVFLIGDAATQVKATTYGGIIYGLLAAQYLAEDK
                     ETYEQRMNAKLGKDLKLSLRMRGFINSMTEEQAEELIGIFERDQNREVIGRHDRDFPS
                     RFIVQLLMKETKLWRLGADVLRRRIGPRRILDHG"
     gene            complement(2037..2438)
                     /locus_tag="VFH83_11710"
     CDS             complement(2037..2438)
                     /locus_tag="VFH83_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487080.1"
                     /translation="MTETFRKLNYKDQRPILVLGAPQSFSGEIASMNAETEVRTSPAK
                     GARYAFVIAFAATKSDMKKAGAQVVKLLDGDAVCWFAYPKPSSRAIKTELNRDLLWEL
                     LMPLGIRPVRQIAIDADWSALRFRAEKYVKS"
     gene            2555..>3062
                     /locus_tag="VFH83_11715"
     CDS             2555..>3062
                     /locus_tag="VFH83_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487081.1"
                     /translation="METIGLIAAMEREAAPLRRLVADRTRLRLRGISGFRFRLGDREC
                     RLLVSGVGPLRAGSAARALLRDAPPDVLVSFGVAGGAERGLRVRDLVIVRSVQLLDHD
                     ELVQPLALAVLASGALKLVRSVAEGHGVQTFEGTAITTSGSQGIPGGRGAAVHPILEM
                     ETHAIAIAA"
BASE COUNT          487 a          993 c         1010 g          572 t
ORIGIN      
        1 tctcccaggg tctggctctc gtagttggcg agccgcagct cgctgcccag gtcgatgatc
       61 tggccgatga cggtgatttc cgccaggtcg ttgcgctgct ccgccgcgga ggcccgccgc
      121 gcaatggcgg cctgcaggtt cgcggtgttg cgggtcaggt acgtcgcgca cgcgttcatg
      181 aaggaggaag cggccgtgtc ctgggtctgg tgcagatcgt ggatcttgtt gatggtggtc
      241 tcggtctggt tgaagagcag cttgtaatcg ttgacctttg tggtggcatc caccgagttc
      301 ttcttcaata cgaccagccg cgggaacttg accgccaggc gctccgcgtc cgtgagggcc
      361 ttctccatgt ccgtgaggtt ctgccgcgcc ttggcgagct catccgggtt tccggactgg
      421 gcgtacccgc gcatggtgta catcgtgagg agggcgctgc gctcgatgtt gttggccacg
      481 gcgacctggg ggacgatctc gtccgaaagc cgccccgcgt ccccctggac ccccatcatg
      541 ctgacgaccg ctactgctcc gagggccatg gctatggcaa tgacgagccc aaagccgacg
      601 ccgattttgc cagcaagcct caagtttctc atctcggttt cgctcctgga tccgtgccgc
      661 ggagcatgac tcgacccggt tccccaggcg tagctccgct catggagaat gcaagctccg
      721 tgccaggtag atggggacga gtgggcgcga tcgaaaccta actagttact gggtaagcac
      781 ttgctctcga ggatttcgag gccccttccc acggtcgact tctctggaac agtgttccac
      841 gcgaacaggg gttggtgcag ccggcgtgaa cactatggag cagccgcgga aagcaacccc
      901 accgaccgcg ccgggctatc cgtggtccag aatccggcgg ggaccgatcc gccggcgaag
      961 cacgtccgcg ccaagccgcc agagcttggt ctctttcatg aggagctgga cgatgaaccg
     1021 ggatgggaag tcccgatcgt gtctgccgat gacctcgcgg ttctggtccc tctcgaaaat
     1081 gccgatgagc tcctccgcct gctcctcggt catcgagttg atgaacccgc gcatgcgaag
     1141 gctcagcttg aggtctttcc cgagcttggc gttcatccgc tgctcgtagg tctctttgtc
     1201 ctcggccaga tactgagcgg ccaggagacc atagatgatc ccgccgtacg tggtggcctt
     1261 cacctgagtg gccgcgtctc cgatgaggaa cacgtcctca ccccggagcc gaagcttctg
     1321 ccggggattg tagaccggga tgacaccgct ctggtcttcc aggatcttcg aggctcccag
     1381 cagccggtgg tagtctctgc gaaggcctgg agcgtccggc ccgatgacgc cgacccgggc
     1441 gatgcgctgg ctctccggca cgatccagga gaactcgccg agggccagtc ggatctccgt
     1501 cacctccggc tccagcccgt cgtactcaca gcgggactgc caccccatca cgaactgacg
     1561 gcgatcgtac atcccggccg agcgggcaac ggcgctgaac ggcccgtcgg cgccgacgat
     1621 catctccgcc gcgtaggagc cccggttggt gcggatctgg aaaccgcccg cgcaacgctc
     1681 gaagcccagg aaatgctcgc cgaggtgcaa ggtggcgccg tggtccaggg cccgccgcag
     1741 gaggtagcga tcgaaagccc cccggtccag gatcatgttc accttgtcca ggtcgacgta
     1801 caggcttttg ccgctcggcg cgacgatctt gaacttgcgg atcctgctta caacgaggct
     1861 ctccgggatc tcgccgagca cccgcttgac cgaatccgta aggatcccgg tgcaggacac
     1921 cggcgatccg acggtgtcgt gctcttcgaa gaggtggacg tcgccctggg ccagggatgc
     1981 gtagtagcat cccgcgggcc cggctccaat cacggcgatc acgggctgga tcctcctcag
     2041 gactttacgt acttctccgc tcggaaccgc agcgcgctcc aatccgcgtc gatcgcgatc
     2101 tggcgaacgg ggcggatccc gagaggcatg agcagctccc agaggaggtc tcggttcagc
     2161 tcggtcttga ttgcgcgaga cgagggcttg ggatacgcga accagcacac cgcatcgccg
     2221 tcgaggagct tgaccacctg ggcgcccgct ttcttcatat ccgacttcgt agcggcgaac
     2281 gcgatgacga aggcgtaccg agcccccttg gcgggactgg tccgcacctc ggtctcggcg
     2341 ttcatggacg cgatctctcc cgagaagctc tgcggggcgc ccagcacgag gatgggtcgt
     2401 tggtccttgt agttgagctt tcggaaggtc tcggtcatac cgtcgagcag tatactctcg
     2461 ggcgggacgg aactcaaggc aacggaccgg gctgaccggg ccggtgaacc ggcggcttgc
     2521 ggtccgggca gccgccaact atcctgcgca tggcatggag acgatcggac tgatcgcggc
     2581 catggagagg gaagccgctc cgctccgtcg gctggtggca gaccggacgc gcctgcggct
     2641 gcgcggcatt tcgggcttcc gtttccgact gggggaccgg gagtgccggc tcctggtgag
     2701 cggcgtgggg ccccttcgag ccggttcggc ggcccgcgcg ctgctccgag atgccccacc
     2761 ggatgtgctc gtctccttcg gcgtggccgg cggcgccgag cgggggctgc gggtccggga
     2821 cctggtcatc gtccgatcgg ttcagctcct cgatcacgac gagctggtgc agcctttagc
     2881 gctcgccgtc ctggcgtccg gggcgctgaa gctcgtacgc tccgtggcgg aaggccacgg
     2941 cgtacagacg ttcgagggga ccgccatcac tacctcaggc tctcagggca tcccgggcgg
     3001 caggggggcc gccgtccatc cgatcctgga gatggagacc cacgccatcg ccatcgctgc
     3061 cc
//
LOCUS       DASRZS010000480         7260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2569743, whole genome shotgun sequence.
ACCESSION   DASRZS010000480 DASRZS010000000
VERSION     DASRZS010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2569743"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VFH83_11720"
     CDS             complement(<1..474)
                     /locus_tag="VFH83_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487082.1"
                     /translation="MSAGREQPGTAGSDVAVLEARGLGWAVRGKQILSAVDLKLRAGE
                     LVGVIGPNGAGKTTLLRLLTGVLQPTEGSVYLRGRPLGPLGPRARARSLAFMTQDVAQ
                     AFSFTVLEVLLMGRYPHLGRFEQESAEDRERARRTLSYVGLSGLEERSFSELSGGE"
     gene            complement(471..1502)
                     /locus_tag="VFH83_11725"
     CDS             complement(471..1502)
                     /locus_tag="VFH83_11725"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET6487083.1"
                     /translation="MRSRRSLTIAILCVALAAALVLAVGIGAVPVPPISLLKLILHSV
                     GLQSVPLPDPRDVTIILYLRLPRALAAVLVGSTLAACGVVMQGLFRNPMASSEILGVS
                     AGGSLGAVIAITTGLAGLSLLAMPVFTIAGALLSAAAIYLLSTRKGTTSLLYIVIAGM
                     AISSFFNGLTSALLLFAQQYQMSQYIFWTMGGLDGRGWQNIEVSAPVLVVGIVVLSFF
                     TRELNLFTMGEEGAQTLGVAVERTKRILLAVAAVLTGVAISVAGPIGFIGLLVPHFFR
                     LIVGADHRALLPAASLGGAVFLVLCDLVGRSIAPPFEIRVGIITAVLGSPYLLSLIVR
                     TQRRGPRAA"
     regulatory      complement(1688..1813)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1982..2176
                     /locus_tag="VFH83_11730"
     CDS             1982..2176
                     /locus_tag="VFH83_11730"
                     /inference="COORDINATES: protein motif:HMM:NF039796.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HET6487084.1"
                     /translation="MQCANSEKNARRCTCTYPGCSRHGICCDCVAYHRGAGELPGCLF
                     APAEEKTYDRSVRYFVASRR"
     gene            2183..2791
                     /locus_tag="VFH83_11735"
     CDS             2183..2791
                     /locus_tag="VFH83_11735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET6487085.1"
                     /translation="MLDLVILSLGFMIHMWALPYSFKTILFDTIVRLAQAGFGVTILL
                     QLDPGTLVPSTVGLITGGGVGLACFFFQVVWNRGIALRRSDITRSFVASQLLILLFQV
                     PVEELFYRGVFFTVLAAIWGPFTALLLSAALSTMITVVSSRRTIVWVGSGLMGVLCGL
                     GFYWSQCIWAPILIHTLNDLGYVSLNEKRNVFEERNRGKRRP"
     gene            complement(2794..3663)
                     /locus_tag="VFH83_11740"
     CDS             complement(2794..3663)
                     /locus_tag="VFH83_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487086.1"
                     /translation="MKMKTIFLLLNAVLTAAFAVIFLTPLFLLGGDWFTVFWSRNWPI
                     AIVFVVTLGAVDAYFLLNWRLFRGLEREDWATVAGILERRVFSGRWMLPGQLRLLLNT
                     YLVASNTDAIRALEAYLAQHRPSLIPRFALAFGIPHLIAKDPSDPEEFFRRLLGNRRL
                     SDRDWVRWNHAFSLLQLKREGEARSELSQLAQSTKEPVLLLLVLYLVEVTGGLEGQRD
                     LLTARRTELRSRYAPAAMQRAIEQSGANLQVLVLSRLLQDARDWLYREDTAPTEPPKV
                     VSAEGAPPEGPAR"
     gene            3750..4913
                     /locus_tag="VFH83_11745"
     CDS             3750..4913
                     /locus_tag="VFH83_11745"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6487087.1"
                     /translation="MKTVPTSSLAPGSFIDQQVFLDKGYILLTPDSPVTPELVGRLQK
                     WKYAEVFTEGKTREAPADGVSVAAQVPTAKTIDQNIEDAKLAESARTFHQNLSGFTAA
                     LLAKYIADGVIPLAEVTEWIKKTIRIVHDARDFILLCLDSEADADHFLVSHAVNSTIL
                     SVAIGDFLKAPPHRLIELGQSGLLHEIGMFRLPAELRRSKSAYSPEERKVMSAHTVAG
                     YRVLKGVSAPENVALAALEHHERMDGSGYPRSLTADKITEYARIVAVTCSYDAMVSRR
                     PFREGVRDGHSAIKELLQTSRKQYDDRILKAIVYTLSIYPVGTAVLLSNNQRGVVVRT
                     DSAKPRCPVVKVLADQDGRPVVEPQLVRVSENHGVSISRVLKPEEVGDLRQRA"
     gene            4910..6568
                     /locus_tag="VFH83_11750"
     CDS             4910..6568
                     /locus_tag="VFH83_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HET6487088.1"
                     /translation="MNVRVSLPGGLTRDYPAGTPVRQAIESPDSTDPGFPLVAALLNN
                     EVVWLDHPLSFNCAVAPVDLGSKAGTVIYRKSLCFLLGMAAGRTFPKRRLIIGHSLGQ
                     GYYYHFDGLPKVGRDDLVRLEASMREIVARDLPIRSLYLSYHEAMEYFEKHNQPEAVL
                     LLQNHNDPQVEVQSCDGLLELSHGPLAPSTGLLSAFGLQEYEPGFLLRYPPSENPLHL
                     GPFVENPVLFSIYREYKNWGKVLQVGSVGRLDELIRDGRIQEFVQIAEALHDKKIAEI
                     ADRINEAREQVKVVLIAGPSSSGKTTFSKKLMIQLRVVGRNPVTLSLDDYFKPSSATP
                     RDEEGRPDFEALSALDVELLNDHLVRLLSGQEVETPLFDFHTGSRKPRGQAMKLPDRA
                     VLILEGIHGLNDALTPLVGRGQKYKVYVSALTQLNLDDHNRISTTDNRLIRRMVRDNQ
                     FRGHSALKTLSMWPSVRRGEDRNIFPFQNTADSAFNSALDYELAVLKTYAEPLLATVK
                     PDSPEYQDARTLLRFLANFAPLHPRWVPTTSILREFIGESAFRY"
     gene            complement(6587..>7260)
                     /locus_tag="VFH83_11755"
     CDS             complement(6587..>7260)
                     /locus_tag="VFH83_11755"
                     /inference="COORDINATES: protein motif:HMM:NF022449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HET6487089.1"
                     /translation="NIVVPSFAIERSQEVLYYLNRLLRADRIPHLLVFLDSPMATKVT
                     EIFGHWPAYLDEDMKRLIRENQSPFDLPGLVMTRTSEESKTINRINGSAVIIAGAGMC
                     TGGRIKHHLARNLSRSESTILFVGYQAEGTLGREIVSGARQVRILGETQPVRARVARL
                     EGLSGHADRDELLRWARCLKQPPRQTFVIHGEPKTAEHFRATLEQSLGWNASVPSSEQ
                     SAVLG"
BASE COUNT         1314 a         2425 c         2336 g         1185 t
ORIGIN      
        1 ctccccgccg gacagctccg aaaagcttcg ctcctccaga ccagacagac ccacgtagga
       61 aagggtccgc cgggcgcgct ctcggtcctc cgcggattcc tgctcgaacc ggcccagatg
      121 agggtagcgc cccatcagaa ggacttccag gaccgtgaac gagaacgcct gggccacgtc
      181 ctgggtcatg aacgccagcg agcgcgcccg ggcgcgaggg cccagagggc ccaacggccg
      241 gccccgcaag tagacgcttc cctcggtggg ttgcagaacg cccgtgagca gccgaagcag
      301 ggtcgtcttg ccggcgccgt tggggccgat cacccccacc agctcaccgg cccggagctt
      361 cagatcgacc gcggagagga tctgtttgcc ccggaccgcc cagcccaggc cgcgcgcttc
      421 caggaccgcc acgtcggagc cggccgttcc cggctgttcc cgtcccgcgc tcatgccgcg
      481 cgcggtcccc ggcgctgagt gcgcacgatc aagctaagca ggtacgggct cccgagcacc
      541 gcggtgatga tcccgacccg gatttcgaag ggcggcgcga tggatcggcc aaccaggtcg
      601 cagagaacca ggaacacggc gccgcccagg gaggcggcgg gaaggagcgc tcggtggtcg
      661 gctccgacga tcaggcggaa gaagtggggc accagcagcc cgatgaagcc gatgggcccg
      721 gccacggaga tcgccacgcc ggtcagcacc gcggccaccg ccagcaggat cctcttggtg
      781 cgctccacgg cgacgcccag cgtctgagcc ccctcctccc ccatcgtgaa gaggttcagc
      841 tcccgggtga agaacgagag caccacgatg cccacgacca gcaccggcgc ggagacctcg
      901 atgttctgcc agccgcggcc gtccagacca cccatggtcc agaagatgta ctggctcatc
      961 tggtactgct gggcgaatag aagcagggcg gaagtcaggc cattgaagaa ggaagagatc
     1021 gccataccgg caatcacgat gtacagaagg gaggtggtgc ccttgcgcgt agacaggagg
     1081 tagatcgccg cggcggagag aagcgctccg gcaatcgtga agaccggcat cgccaggaga
     1141 gagagaccag ccagacccgt ggtgatggca atcacggcgc ccaggctccc gccggccgac
     1201 acccccagga tctcggagct cgccatgggg ttgcggaaga gcccctgcat cactacgcca
     1261 caggcagcca acgtcgagcc caccagcacc gcggccaggg cacgcgggag ccgcaggtag
     1321 aggatgatgg tgacatcccg cggatcgggc agagggacgg attgcaggcc aacggagtgc
     1381 aggatcagct tcaacaggga gatgggcggc acgggtacgg cgccaatccc gaccgccagc
     1441 accagggcgg cggccaacgc gacgcagagg atcgcgatgg taagggaccg tcgagacctc
     1501 acgcgggcgg gctctggaga gtgacgccgg tctgcaggca ctgggttcgc acgctgagtc
     1561 gtgttcgccg ccagatacgt tctacgggac cgagggacgg ctctggggag acggcgcttc
     1621 tcatggaagg cctcctgcaa gtgcatcgcg tgcaggcacc gcatggcggt gtgccgctgg
     1681 ggtcccaacg tttcctggct tccgggtcac cctcccgccc cgccttccca ggctgtgctg
     1741 cccagtggcc cgttggggcg atcgtcgccg gtcacagtgg cgcgtccgtg gcggagttgc
     1801 accgccttcc gttgcaggac cgagcgcact atatctcgcg gtctctggaa gggtcaagca
     1861 agcgctcgat ccggtgcgtt tgttcgcgga gctctgctcc tccgtgagct ccgtgggttc
     1921 cgccctgccg gttccacctc gcgcgcttgc gcaggtacga caaaaccgat acacaggagc
     1981 gatgcagtgc gccaattctg aaaagaacgc cagacgctgc acctgcacgt acccggggtg
     2041 ttcccggcac ggcatctgct gtgattgcgt ggcctaccac cggggcgccg gggagctgcc
     2101 ggggtgcctt ttcgcgccgg cggaagagaa gacgtacgat cggagcgtca ggtactttgt
     2161 agcgagccgg cgatagcgcg tcgtgctcga tctcgtcatc ctctccctgg gcttcatgat
     2221 ccacatgtgg gccctgccgt actcgttcaa gacgatcctg ttcgatacga tcgtccgact
     2281 ggcgcaggcc ggcttcggtg tgaccattct gctccagctc gatccaggaa ccctggtgcc
     2341 gtccaccgtg ggtctgatca ccggcggcgg ggtcggcctg gcgtgcttct tcttccaggt
     2401 cgtgtggaac cgcgggatag ccctgcgcag gtccgacatc acgcgcagct tcgtggcatc
     2461 gcagctcctg atcctgctgt tccaggtgcc ggtcgaggag cttttctatc gaggagtctt
     2521 cttcacggtg ctggccgcca tttggggacc gttcaccgcg ctgctcctgt ccgcagccct
     2581 ctcgaccatg atcaccgtgg tctcctcccg gcggaccatc gtgtgggtgg gttccggcct
     2641 catgggagtt ctctgcggct tgggcttcta ctggtcccag tgcatctggg cgccgatcct
     2701 gatccacacc ctcaacgacc tgggctacgt gtccttgaac gagaagagga acgtgttcga
     2761 agagcggaac cgaggaaaac gacgcccgta gcgctatcga gccggaccct ccggcggagc
     2821 gccctccgcg gagacgacct tcggcggttc ggtcggcgcc gtgtcttccc gatagagcca
     2881 atcccgggcg tcctggagca ggcgggagag cacgagcacc tgcaggttcg caccggattg
     2941 ctcgatggcc cgctgcatcg ccgccggcgc gtagcgggat cgaagctcgg tccggcgagc
     3001 cgtcaggaga tcccgttgtc cctccaagcc gcccgtaacc tccacgagat acagcaccag
     3061 caagagaagc acgggctcct tcgtggactg ggcgagctgg gatagctcgg acctggcctc
     3121 cccctcgcgc ttcagctgca agaggctgaa cgcgtgattc catctcaccc agtcccggtc
     3181 cgaaagcctt cggttcccca gaaggcggcg gaagaactcc tcgggatcac tggggtcctt
     3241 ggcaatcagg tgagggatgc cgaatgccaa ggcgaaccgc gggatgagcg acgggcggtg
     3301 ctgcgccaga taggcctcca gcgcccggat ggcgtccgtg ttggaggcga ccaggtacgt
     3361 attcagcagg agccgaagct gcccggggag catccagcgc cccgagaaaa cccgccgctc
     3421 caggatgccg gccacggtag cccagtcctc acgttccagg ccccggaaga gccgccagtt
     3481 cagaaggaag taggcatcca ccgccccgag agtcaccacg aacacgatgg cgatgggcca
     3541 gttgcgggac cagaacacgg tgaaccagtc accgcccagg aggaacaggg gcgtgaggaa
     3601 aatgacggcg aacgccgccg tcagcaccgc gttcagcagg agaaatatcg ttttcatttt
     3661 catgggaaat ccctataata ggaaggtagt gtacggagaa cggcggtcga ggtcaaaggc
     3721 ctgggagtgg ccgccaagga acacggcgga tgaaaacggt ccccacatca tcgctggcac
     3781 ccggtagctt tatcgaccaa caggtgttcc tggacaaggg ctacatcctc ctcacgccgg
     3841 actccccggt cactccggag ctcgtcgggc ggcttcagaa gtggaagtac gccgaggtgt
     3901 tcaccgaggg caagacccgg gaggctcccg ccgacggcgt gagtgtcgcc gcacaggtgc
     3961 cgacggcgaa gactatcgac cagaacatcg aggacgccaa gctggcggag tcggcacgca
     4021 cgttccacca gaacctcagt ggattcaccg cagcgctcct ggccaagtac atcgcggacg
     4081 gggtcatccc gctggcggag gtcacggagt ggatcaagaa gaccatccgg atcgtgcatg
     4141 acgctcgaga cttcatcctg ttgtgcctcg actcggaggc ggacgcggat cacttcctgg
     4201 tctcccacgc ggtgaacagc accatccttt ccgtcgccat cggggacttc ctgaaggcgc
     4261 ctccccaccg cctcatcgag ctgggccagt cagggctgct ccacgagatc ggcatgttcc
     4321 gtctgccggc ggagctccga cggtcaaagt ccgcttacag ccccgaggaa cgcaaggtga
     4381 tgagcgccca caccgtggcc ggctaccgcg tcctgaaggg ggtctcggcg ccggagaacg
     4441 tggctctggc cgcgctggag caccatgagc gcatggacgg cagcgggtat ccccgaagcc
     4501 tcaccgcgga caagatcacg gagtatgcca ggatcgtcgc ggtgacctgc tcctatgacg
     4561 ccatggtgtc caggcgtccg ttccgcgaag gggtgcggga cggacactcc gccatcaagg
     4621 agctgctcca gaccagccga aagcaatacg acgatcggat cctcaaagcc atcgtctata
     4681 ccctctcgat ctacccggtg ggcacggcgg tgctcctctc caacaaccag aggggcgtgg
     4741 tcgtgcggac cgattcggcc aagcctcggt gcccggtcgt gaaggtgctg gcggaccagg
     4801 acggaaggcc ggtggtggag ccgcagttgg tccgcgtctc ggaaaaccac ggcgtgagca
     4861 tcagccgggt gctcaagccc gaggaggtgg gggatttgag gcagcgcgca tgaacgtgcg
     4921 cgtcagcctg cccggcggcc tcacccgcga ctatccggcc ggcacccccg ttcgacaggc
     4981 catcgagagc cccgactcga cggacccagg cttccctctg gttgccgcgc tcctgaacaa
     5041 cgaggtggtc tggctcgatc atcccctgtc cttcaactgc gcggttgcgc ccgtcgacct
     5101 gggcagcaag gccggcaccg tgatctaccg caagtccctc tgtttcctcc tgggcatggc
     5161 cgcgggaagg accttcccca agcgacgtct catcatcggg cactccctgg gtcagggcta
     5221 ctactaccac ttcgacgggc tgcccaaggt gggtcgggac gacctggtcc gcctggaggc
     5281 ctcgatgcgg gagatcgtgg ccagagacct gcccatccgg tcgctctacc tctcctacca
     5341 cgaggccatg gagtacttcg agaagcataa tcagccggaa gcggtcctgc tcctccagaa
     5401 ccacaacgat ccccaggtcg aggtccaatc ctgcgacggc ctcctggagc tctcccacgg
     5461 tccgctggcg ccttccaccg ggctgctctc cgccttcggt ctccaggagt acgagcccgg
     5521 cttcctgctc cgctacccac cgtcggagaa tcccctccac ctcgggccct ttgtcgagaa
     5581 tccggtcctc ttcagcatct accgggaata caagaactgg ggcaaggtcc tccaggttgg
     5641 ctccgtggga aggctggacg agctgatccg cgacggcagg atccaggagt tcgtccagat
     5701 cgccgaggcg ctgcacgaca agaagattgc cgagatcgcc gaccgcatca acgaggcgcg
     5761 ggagcaggtc aaggtggtgc tgatcgccgg gccgtcctca tccgggaaga ccacgttctc
     5821 caagaagctc atgatccagc tccgcgtggt gggacgaaac ccggtcacgc tctccctcga
     5881 tgactacttc aagccgtcgt cggccacgcc gcgggacgag gaaggacggc cggacttcga
     5941 ggccctctcc gcactggatg tggagctgct caacgaccac ctggttcgtc tgctctcggg
     6001 gcaggaggtg gagacgcctt tgttcgactt ccacaccggc agccgaaaac cgcgcggcca
     6061 ggcgatgaag ctgcccgatc gggcagtctt gatcctggaa ggcatccacg ggctcaacga
     6121 cgccctgacg cccctggtcg gtcggggaca gaagtacaag gtctacgtgt cggccctcac
     6181 gcagctcaat ctggatgacc acaaccggat ttccaccacg gacaaccgtc tgatccgccg
     6241 catggtccgg gacaaccagt tccgcgggca ctcggctctg aagaccctgt cgatgtggcc
     6301 ttccgtgcgc cgtggggaag accgcaacat cttcccgttc cagaacaccg cagactccgc
     6361 gttcaactcc gcgctggact acgagctcgc ggtcctgaag acgtacgcgg agcctctcct
     6421 ggccacggtg aagccggact cacccgagta ccaggacgcg cgcaccctgc tccgcttcct
     6481 ggccaacttc gcgccgctgc acccgcgatg ggtgcccaca acgtcgatcc tgcgggagtt
     6541 catcggggaa agcgctttcc gctactgagg cgcgccgggc cgcggatcag cccaggaccg
     6601 ccgactgctc gctggacggg accgaggcgt tccacccgag gctctgctcc agggttgccc
     6661 ggaaatgctc cgcggtcttc ggctccccgt gaatgacgaa ggtctgccgg ggcggctgct
     6721 tcagacatcg tgcccaccgc aggagctcat cccgatccgc gtgtcccgaa agcccctcca
     6781 ggcgcgccac ccgcgcgcgc acgggttgtg tctctcccag gatccgcacc tgtcgggcgc
     6841 cgctcacgat ctccctcccc agcgttcctt ccgcctggta gcccacgaag agaatcgtgc
     6901 tctccgaccg ggacaggttg cgggcaaggt ggtgcttgat ccggcccccc gtgcacattc
     6961 ccgcgccggc gatgatgacc gcgctcccgt tgatgcggtt gatcgtcttc gattcttccg
     7021 aggtccgggt catcacgagg cccggcagat cgaacggcga ctggttctcg cggatgaggc
     7081 gtttcatgtc ctcgtccagg taggccggcc agtgaccgaa gatctccgtc accttcgtcg
     7141 ccattgggct gtccaggaat acgaggaggt gggggatgcg gtccgctcgc aagagacggt
     7201 tcaggtagta gagcacctct tgggatcgct cgatggcgaa gctcgggacc acgatgtttc
//
LOCUS       DASRZS010000481         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4877729, whole genome shotgun sequence.
ACCESSION   DASRZS010000481 DASRZS010000000
VERSION     DASRZS010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4877729"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VFH83_11760"
     CDS             <1..754
                     /locus_tag="VFH83_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487090.1"
                     /translation="IALAATLLIGLLPGLLSGFLVGRLKVTPFLATFATGGIVYGISL
                     LLTGGGETKGVPNLANFIGNSYFMYWAPGRGLSFFTRPEVARGTRVLEIIPSMLVVAA
                     VVVAVAAWILNRTRFGRHTYAIGGNEDAAVRAGINIRRHYLVIYTASAFFSTLAGLIY
                     MLEYITGKPDAGTSYLLDSIVGVVIGGASLYGGAGTVWGTILGCLILSVLETGLRMAG
                     VPTFDKFIAVGLILILAVLIDQFFPELIHKED"
     gene            758..1510
                     /locus_tag="VFH83_11765"
     CDS             758..1510
                     /locus_tag="VFH83_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6487091.1"
                     /translation="MGYLLEVRNIVKRFGGLTAVNNVSMGVSAGEVVGLLGDNGAGKS
                     TLIKVISGVYRAEEGKILFKDREVEVASPMDALAMGIETIYQDLALAENLNTPANIFL
                     GREKMKKSLGVLNVLDHEYMHEESRKVLEKLDILIPSLRNKIKALSGGQRQAVAISRS
                     IYWDAKFLIMDEPTAALGVAEQKKVLDLVNRLKDHGIGIIVISHQMHDVFSVADRLVI
                     MRRGEKVAELVTRQTNPDEVVSFIVGSETVRQ"
     gene            1547..>2442
                     /locus_tag="VFH83_11770"
     CDS             1547..>2442
                     /locus_tag="VFH83_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487092.1"
                     /translation="MKLFTKTLLFFVGVIAVQAVLSVLLVNNITRRADLADAGRELGD
                     EATILADSFNSWKRQIWISLIGMTNDQRLGALLAASRGSATQEPLMGALKGFVFVSKI
                     DLLAVGDPRARLLDLIPVTYNTFELEDLARLSNEKSHPYLELRLIHGVLCLIGITRVS
                     AAGEPAHDLFLIKRLDGEFCGQLTLNRKSQVGLYLGPRCLVSSFPPAIPASELEAEPV
                     SGAYRQLFDQGIGGQRRNVAFQWAGTISAGPEAGVGDHGELYLATFLPNAPYAERSLQ
                     ASRAVLLVFLAAALVTIVLSLFL"
BASE COUNT          409 a          795 c          809 g          429 t
ORIGIN      
        1 catcgcgctg gcggccacgc tgctgatcgg cctgctgccg gggctgctga gcgggttcct
       61 ggtggggcgc ctgaaggtga cgccgttcct ggccaccttt gccaccgggg gcatcgtgta
      121 cggcatctcc ctgctgctca ctggcggggg agaaaccaag ggcgtgccga acctcgccaa
      181 cttcattggc aacagctact tcatgtactg ggctccgggc cgcgggttgt cgttcttcac
      241 gagacccgag gtggcgcgcg gcacgcgcgt gctggagatc atcccgagca tgctggtggt
      301 cgccgccgtg gtggtggccg tggccgcctg gatcctgaac cgcacccgct tcggccggca
      361 cacgtacgcc attggcggca acgaggacgc ggccgttcgg gccgggatca acatccggcg
      421 ccactacctg gtgatctaca ccgcctcggc gttcttctcc acgctggccg ggctcatcta
      481 catgctggag tacatcacgg gaaagccgga cgcggggacg tcgtacctgc tggactccat
      541 cgtgggagtg gtcatcggcg gtgcgagcct gtacgggggc gcggggaccg tctggggcac
      601 aatcctcggc tgcctcatcc tctccgtcct ggagacgggg ctccgaatgg ccggcgtccc
      661 gacgttcgac aagttcatcg cggtggggct catccttatc ctcgccgtat tgatagatca
      721 gttcttcccc gagctgattc acaaggagga ctagctgatg gggtaccttc tggaagtccg
      781 caacatcgtc aagaggttcg gcggcctcac cgccgtgaac aacgtcagca tgggggtgtc
      841 cgccggcgag gtagtgggcc tgctgggaga caacggtgcg ggaaagagca ccctgatcaa
      901 ggtcatctcg ggggtctacc gggcggagga gggaaagatc cttttcaagg accgcgaggt
      961 ggaggtcgcc tctcccatgg acgcccttgc catggggatc gagaccatct accaggacct
     1021 ggcgctggcg gagaacctga atactcccgc gaacatcttc ctcggccggg agaagatgaa
     1081 gaaatccctg ggcgtcctca acgtgctgga ccacgagtac atgcacgagg aaagccgcaa
     1141 ggtcctggag aagctggaca tcctcatccc gtccctgcgc aacaagatca aggcgctctc
     1201 cggcgggcag cggcaggccg tggccatctc gcggtccatc tactgggacg cgaagttcct
     1261 gatcatggac gagcccacgg cggccctcgg cgtggccgag cagaagaagg tgctggacct
     1321 ggtcaaccgc ctcaaggacc acggcattgg catcatcgtg atcagccacc agatgcacga
     1381 cgtgttctcc gtggcggacc gcctggtcat catgcgccga ggggagaagg tcgccgagct
     1441 ggtgacccgc cagacgaacc cggacgaggt ggtgagcttc atcgtgggat cggagaccgt
     1501 ccgacagtag cccaggggga gatccgccgg gaggcctgcg gcacgcatga agctcttcac
     1561 gaagacgctt ctttttttcg tcggggtgat cgcggtccag gcagtgctct ccgtgctgct
     1621 ggtgaacaac atcacccgcc gggcggacct cgcggacgcc gggcgggagc tcggcgacga
     1681 ggccacgatc ctggcggaca gcttcaactc ctggaaacgc cagatctgga tcagcctgat
     1741 cgggatgacc aacgaccagc gcctcggcgc gctgctcgcg gcttcccgcg ggagcgccac
     1801 ccaggaaccg ctgatgggtg cgctcaaggg tttcgtgttc gtctccaaga tcgacctgct
     1861 ggcggtaggc gatccccgcg cgcgcctcct ggacctgatt cccgtgacct acaacacctt
     1921 cgagctggag gacctcgccc ggctctccaa cgagaagagc cacccctacc tcgagctgcg
     1981 gctcatccac ggcgtgctgt gcctgatcgg catcacgcgc gtttccgcgg ccggcgagcc
     2041 cgcccatgac ctgttcctga tcaagcgcct ggacggggag ttctgcggtc agctcaccct
     2101 gaaccgcaag tcccaggtcg ggctgtacct gggaccgcgg tgcctggtga gctccttccc
     2161 tcccgccata cccgcatccg agctcgaagc ggagccggtg agcggggctt accggcagct
     2221 cttcgaccag ggcattggcg ggcagcggcg caacgtggcg ttccagtggg ccggaacgat
     2281 ttccgcaggg ccagaggctg gcgtcgggga ccacggcgag ctgtacctgg ccaccttcct
     2341 tcccaatgct ccctacgcgg agaggagcct gcaggccagc cgggccgtcc tcctggtgtt
     2401 cctggcggcc gccctggtca cgatcgtcct gagcctgttc ct
//
LOCUS       DASRZS010000482         4973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4110660, whole genome shotgun sequence.
ACCESSION   DASRZS010000482 DASRZS010000000
VERSION     DASRZS010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4110660"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1067)
                     /locus_tag="VFH83_11775"
     CDS             complement(129..1067)
                     /locus_tag="VFH83_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET6487093.1"
                     /translation="MKRQTAVILACYATIYVVWGSTYYFIRSSVATIPPGWVLAIRWT
                     IGGCILLGYSAARGKLRVRLSFREVLASIALGVLLLLLGNGGITLAERTIDSYIGALL
                     ASSTPILVAVFDGLLLRKRMTVARVLGVIFGCVGVAVFLYNGHSVGSTLSPSLLIGLL
                     GAVAWALATSLGHRFPVSGDNTVNSGIQMLFVGGITLAGSLLLGPPVREVTQAISAAS
                     LVGVLYLAVIGSLAFSAFTYLVAREPAERLVSYALVNPLIALVIGLGLAGETATPYLG
                     IGVPLVLVGLAFMVYGERFFARIRPKSAESRRSSAP"
     gene            1229..3016
                     /locus_tag="VFH83_11780"
     CDS             1229..3016
                     /locus_tag="VFH83_11780"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET6487094.1"
                     /translation="MTASELRRKYIQFFQGKGHAEISGKSLIPENDPTVLFTTAGMHP
                     LVPYILGEPHPAGKRLVDVQKCIRTDDIDEVGDPSHLTFFEMLGNWSLGDYFKNDAIR
                     FSYEFLTSPQWLGFSPDVLSVTVFAGDAEVPADQEAAGVWRSLGIPESRIFFLPRKDN
                     WWGPAGQTGPCGPDTEMFIDTGAPPCGPECKPGCSCGKYFEVWNDVFMQYNKTAAGKY
                     EPLAQRTVDTGMGVDRTIAMMQRKKTVFETELFAPVIARLSQITGASYGNDGDADHSF
                     RIITDHTRAATFILGDDRGVKPSNLGQGYILRRLIRRAIRHGRKLGLEGGFLGKLAEG
                     FIDIYRDVYPEVAGRSSMITSELAQEEERFLETLQKGEKEFEKLLPNLEKNPQKIIPG
                     RVAFRLYDTYGFPVEITEELAREHGMTVDRTGFDEAFQKHQELSKAGAEKVFKGGLAD
                     HSEMTTRLHTATHLLHKALRTVLGDHVAQKGSNITVERLRFDFSHPQPMTEEEVRKVQ
                     EIVNQQIARDLPVSMEVMTLEQAKAAGAIALFGEKYQEKVKVYTIGDFSKEVCGGPHV
                     QHTGELGKFVIQREQSSSAGVRRIRAVLE"
     gene            complement(3085..4185)
                     /locus_tag="VFH83_11785"
     CDS             complement(3085..4185)
                     /locus_tag="VFH83_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6487095.1"
                     /translation="MSGDERSPGPVQVSISELSKRGYQLLKEDHIAEAEENFRRILEI
                     EPQNNYALVGMGDAARKRKSYHEAIRYYEECLGYHVGNKYALVGLADSYRSLKQFHRA
                     IEIWEKYLAQGDSDVTVLTRIADAYRKVLDFSRSKEIYHKILEIDPGNPYALTGLGYL
                     HFDLKEYREALTYWERMHEKDPKNIWVLTSIGNCYRKLKTYEKGIPYFKKALEIQPNN
                     FYALFGLADCYRGMNDPQESLFYWQKILDADPDNKVILTRIGDSYRAMNDLDTAREFY
                     SKALNIEFDSYAVLGLALINKEKGNYLEAIESLSGLLVNDAKNPRLYVEIAQCWQALE
                     HRDKALEVLMRAQRMGVKNLGIAEMLEKLQKP"
     gene            complement(4182..4532)
                     /locus_tag="VFH83_11790"
     CDS             complement(4182..4532)
                     /locus_tag="VFH83_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487096.1"
                     /translation="MSAEDFLKKLPSGGFLKVAKGVPSSLTGEQKAALIRKGNALFNK
                     GEFDLAKRIFITTGYSDGLIRIGDRYAKAGQPLEALRMYWMAPSPDKKAALVEKIAQV
                     IKQWLLEGKGTKAQ"
     gene            complement(4609..>4973)
                     /locus_tag="VFH83_11795"
     CDS             complement(4609..>4973)
                     /locus_tag="VFH83_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HET6487097.1"
                     /translation="VLGQALSGLLERAGAVARFGHAVIPMDEALAEATIDVCGRPTLY
                     WRAEFPQERAGAFDLSLLREFFSGLSAKAAISLHVEVRRGENSHHMAEAAFKALGRAL
                     SQAYAQTDQEMSSKGQIG"
BASE COUNT          919 a         1618 c         1548 g          888 t
ORIGIN      
        1 gggccacggc agacggaggg ccgccgtggt gagcagcgcc ggcaccgcga tccccagggc
       61 ggcgcgccgc cacaggcggg cgtcggcgcg caacaccccg cgctccgacg ctcctggagc
      121 ttctcccctc acggcgcgga agacctccgc gactccgcgg acttcggcct gatcctcgca
      181 aagaaccgct cgccgtagac catgaaggcc agtccgacca ggaccagcgg cacgccgatg
      241 cccagatacg gggtagccgt ctcccccgcg agtccaagcc cgatcaccaa ggcgatcagc
      301 ggattgacga gcgcatagct gaccagccgt tcggccggct ctcgtgccac caggtaggtg
      361 aaggcgctga acgccaggga accgatcacg gccaggtaca gcacgccaac cagggaggca
      421 gcggatatcg cctgagtgac ctcgcggacc ggagggccga gcaacaagct ccccgccagc
      481 gtgatccccc cgacgaagag catctggatg ccgctattga ccgtgttgtc tccgctcacg
      541 ggaaaccggt gccccaggct ggttgccagc gcccaggcaa cggcgcccag cagaccgatg
      601 agcaaggacg ggctcagggt ggatccgacg gaatggccgt tgtacaggaa caccgccacg
      661 ccgacgcatc cgaagatcac gccgagcacg cgcgccacgg tcatccgctt gcgcaggagc
      721 aggccgtcaa acaccgcgac caggatgggg gtcgaggagg ccaggagcgc gccgatgtag
      781 ctgtcgatcg ttcgttccgc cagggtgatg ccgccgttgc ccagaaggag cagaaggacg
      841 cccagggcga tcgaggcgag cacttcccga aacgagaggc ggacccgcag cttgccgcga
      901 gccgccgagt agcccaggag gatacaaccg ccgatggtcc agcggatcgc caggacccag
      961 cccggcggga tggtggctac cgaggatcgg atgaaatagt aggtggaccc ccagaccacg
     1021 tagatcgttg catagcaggc caggatgacg gccgtctgtc gtttcatgga gcgcatccgc
     1081 agtccttgta ccatttccgg ccgggtcggg ccagtgcgga cgctcgcgcg ccgtcggcgc
     1141 aagctcgcgt catagccgca agctcgcgtc atagccgcaa ggtcgcgtca ttgttgacat
     1201 gccccctgcc taccccgtac tttgtgccat gactgccagc gaattgcgcc gaaagtacat
     1261 ccagttcttc cagggaaagg gtcacgcgga gatctcgggc aagtctctga tcccggagaa
     1321 cgacccgacc gtgctcttca cgaccgcggg aatgcatccg ctggtcccct atatcctcgg
     1381 ggagccccac cccgccggca agcggctggt ggacgttcag aagtgcatcc ggaccgacga
     1441 catcgacgag gtcggggacc cctctcacct caccttcttc gagatgctgg gcaactggtc
     1501 cctgggagac tacttcaaga atgacgccat ccggttctcc tacgagtttc tgacctctcc
     1561 ccagtggctt gggttttccc cggatgtcct ctcggtcacg gtcttcgccg gagacgccga
     1621 ggtcccggcg gaccaggagg ctgcgggcgt ctggcgatcc ctcggcatcc ccgagagccg
     1681 catcttcttc ctcccgcgca aagacaactg gtggggacct gccgggcaga ccgggccctg
     1741 cggcccggat acggagatgt tcatcgacac cggcgccccc ccgtgcgggc ccgagtgcaa
     1801 accgggctgc agctgcggga agtacttcga ggtctggaac gacgtgttca tgcagtacaa
     1861 caagaccgcg gcgggcaagt acgagccgtt ggcccagcgg accgtggaca ccggcatggg
     1921 cgtggaccgc accatcgcca tgatgcagag aaagaagacc gtcttcgaga cggagctgtt
     1981 cgctcccgtc atcgcccgac tgagccagat caccggcgcg tcctacggca acgacgggga
     2041 cgcggaccac tcctttcgga tcatcacgga ccatacgcgg gcagccacct tcatcctggg
     2101 ggacgatcgc ggggtgaagc cctccaatct cggtcagggc tacatccttc gccggctcat
     2161 ccgccgcgcc attcgccacg gacggaagct ggggctggag ggcgggttcc tgggcaagct
     2221 tgccgagggg ttcatcgaca tctatcgcga cgtctacccc gaggtggcgg gccgctcctc
     2281 gatgatcacg agcgagctgg cccaggagga ggagcgattc ctcgagacgc tccagaaggg
     2341 cgagaaggag ttcgagaagc tcctgcccaa tctcgagaag aaccctcaga agatcatccc
     2401 gggtcgggtg gcgtttcggc tgtacgacac ctacgggttc cccgtggaga tcactgagga
     2461 gctggcgcgg gagcatggca tgaccgtgga ccggaccggc ttcgacgagg ccttccagaa
     2521 gcaccaggag ctttccaagg ctggcgcgga gaaggtgttc aagggaggtc tggcggacca
     2581 ctcggagatg acgacccgcc tgcacacggc cacccatctc ctccacaagg cgcttcggac
     2641 cgtccttggg gatcacgtgg ctcagaaggg atccaacatt accgtggagc ggcttcggtt
     2701 cgacttctcc catccgcaac cgatgacgga ggaggaagtc cgcaaggtcc aggagatcgt
     2761 caaccagcag atcgcccgcg atcttccggt ttccatggag gtcatgacgc tggagcaggc
     2821 aaaagcggcg ggcgccattg cgctcttcgg ggagaaatac caggagaagg tgaaggtcta
     2881 tacgattgga gacttctcca aggaagtctg cggcgggccg catgtgcagc acaccggaga
     2941 gctcgggaag ttcgtgatcc agcgggaaca gtcgtcctcc gcgggtgtgc ggcggatccg
     3001 cgcggtgctg gagtaacggc gggaggcccc cggctcgccg agctgtcgcc gtccgtggct
     3061 cacgctcggc tccgccgtcc gtggctacgg cttctgcagc ttctccagca tctccgcgat
     3121 gccgaggttc ttgacgccca tgcgctgggc acgcatcagg acctccagcg ccttgtcccg
     3181 gtgctccagc gcctgccagc actgggcaat ctccacgtag aggcggggat tcttcgcgtc
     3241 gttcaccagc aggccggaga ggctctcgat ggcctccagg tagttcccct tctccttgtt
     3301 gatgagcgcc aaccccagga ccgcgtagct gtcgaactcg atgttgaggg ccttgctgta
     3361 gaactcccgc gccgtgtcca ggtcattcat ggcgcgatac gagtcgccga tcctcgtcag
     3421 gatgaccttg ttgtccggat ccgcgtccag gatcttctgc cagtagaaca gagactcctg
     3481 cgggtcattc attccccgat agcagtcggc caatccgaac agcgcataga agttgttcgg
     3541 ctggatctcc agcgccttct tgaagtacgg gatccccttc tcgtaggtct tgagcttgcg
     3601 atagcagttg ccgatggacg tcagcaccca gatgttcttg gggtccttct cgtgcatgcg
     3661 ctcccaatag gtcagcgcct cgcggtactc cttgagatcg aagtgcaggt aacccaggcc
     3721 ggtgagcgcg tagggattcc ccggatcgat ctccaggatc ttgtgataga tctctttgga
     3781 ccggctgaag tccaggacct tgcggtaggc gtccgcgatc ctcgtcagca cggtgacatc
     3841 gctgtccccc tgagccaggt acttctccca gatctcgatg gcgcgatgga actgcttcag
     3901 ggatcggtag ctgtcggcca ggcctaccag ggcgtacttg ttgccgacgt ggtagcccag
     3961 acattcctca tagtaccgga tggcctcgtg gtaggacttc cgcttgcggg cggcgtcccc
     4021 catccccacg agcgcgtagt tgttctgggg ctcgatctcc aggatccgac ggaagttctc
     4081 ctccgcctca gcgatgtgat cctctttcag aagctgatac ccacgtttgg aaagctcgga
     4141 aatcgaaacc tggacgggtc ctgggctccg ctcgtcgccg ctcattgcgc cttcgttccc
     4201 ttcccctcta gcagccattg ttttatcacc tgtgctatct tctccacgag cgccgccttc
     4261 ttgtcagggg atggagccat ccagtacatt cgcagcgcct ccagcggttg gcccgccttg
     4321 gcgtaacggt cccctatccg gatgagtccg tcggagtagc ccgtggtgat gaaaatccgc
     4381 ttcgcgagat cgaactcccc cttattgaac agcgcgttac cctttcggat gagcgcggcc
     4441 ttctgttctc cagtgagcga ggagggaact cccttggcaa cctttagaaa accgccggaa
     4501 ggcaactttt tcaggaagtc ttcggcgctc atccccctcc tgaagaatct ctacttgctc
     4561 aagatacacg aatctcctga cagagtaaat agagcggatc acgcccggct atccgatctg
     4621 accctttgag gacatttcct gatcggtttg cgcgtatgcc tgggatagcg cgcgccccag
     4681 cgccttgaag gcggcctcgg ccatgtggtg gctgttctct ccccgccgga cctcgacgtg
     4741 aaggctgatg gcagccttgg ccgaaagacc gctgaagaac tcccgcagga gtgacaggtc
     4801 gaacgcgccc gctcgctcct gggggaactc cgcgcgccag tacagcgtgg gcctgccgca
     4861 tacatcgatg gtggcttcgg cgagcgcctc gtccatgggg atcaccgcgt gcccgaatcg
     4921 ggccacggct ccggcccgct ccagcagccc ggacagcgcc tgaccgagca cca
//
LOCUS       DASRZS010000483         7484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2953839, whole genome shotgun sequence.
ACCESSION   DASRZS010000483 DASRZS010000000
VERSION     DASRZS010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2953839"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFH83_11800"
     CDS             <1..261
                     /locus_tag="VFH83_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487098.1"
                     /translation="GFNVDLESLTVPGTSTTHVNLYDGTVEGLENRSLRLFSVQYHPE
                     ACPGPHEARYLFDRFVAMMDEGPRDVSTATPGLGVPVPRRPV"
     gene            272..3460
                     /gene="carB"
                     /locus_tag="VFH83_11805"
     CDS             272..3460
                     /gene="carB"
                     /locus_tag="VFH83_11805"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET6487099.1"
                     /translation="MPRRTDIHSILIIGTGPIVIGQACEFDYSGTQACKALREEGFRV
                     VLVNSNPATIMTDPEMADATYIEPLTPAMVEKVIEAEKPDALLPTLGGQTGLNLAIQL
                     ADSGVLDRLGVRLIGADRQAIRKAEDRSLFKESMQRIGIDLPRSGLAHTPEEARRVAS
                     EIGFPLIIRPSFTLGGTGGGIVPDPAAFDEMARRGLSASMVSEILIEESIAGWKEYEL
                     EVMRDRADNVVIVCSIENLDPMGVHTGDSITVAPAQTLTDREYQTMRDAAIACIREIG
                     VDTGGSNIQFAVNPENGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLEE
                     IPNDITRSTPASYEPSIDYCVVKIPRFAFEKFPAADPTLTIQMKSVGEVMAIGRTFKE
                     ALQKAIRGLEIRRPGLSGVGNVAEGLLTEMLARPNAERLYYLYDALQRGWSVERVASL
                     THIDPWFLENVRQIIEFEPTISTANLREAKQMGFSDAQIARRLSTNEAAIRGERQRQG
                     IRPAYKLVDTCAAEFEAYTPYYYSTYDPEDETRASNRKKFLILGGGPNRIGQGIEFDY
                     CCVHAAFALREIGYETIMVNSNPETVSTDYDTSDKLYFEPLTMEDVLAIVDREKPQGV
                     IVQLGGQTPLNLAVGLAQAGVPIVGTSPDAIDRAEDRRRFSELVQKLGLRQPENGSAV
                     KVDEARGIAREIGYPVLVRPSYVLGGRAMEIVWDDASLEHYMQRAVWASPERPVLIDR
                     FIEDAIEVDVDMVSDGRRSVIGGIMEHIEEAGVHSGDAAMVLPPHTLPHWMLEEIRRY
                     TRLLAEELTVVGLMNVQYAVRGKELYIIEVNPRASRTVPFVSKATGVPLAKIAAKVMA
                     GMTLEEAGFTDEVIPRHVSVKESVLPFARFPGVDVVLGPEMKSTGEVMGIDRDFGRAY
                     CKSQLAALQRVPSEGAVFISVKDRDKTQLIVSVAYQLVSVGFTIVSTSGTAAFLKENG
                     IPAEVVRRVSEGAPSVLDLINEGKIQLIINTPTGKIPRRDEVSIRAIATSRGIPVVTT
                     MPGADAYAQGIRAFIRGELGVRPIQRYHAELK"
     gene            complement(3402..4556)
                     /locus_tag="VFH83_11810"
     CDS             complement(3402..4556)
                     /locus_tag="VFH83_11810"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET6487100.1"
                     /translation="MVTGTGTRIGVLGAGAWGTTIAKVLAEKGLDTELWTFEEDLVEE
                     INQRRSNSRYLPGVSLPEGLRACSNLLSVAEDKDFLFIAIPSLYLLENAKKVISCADI
                     REGRTTVSILTKGFLETPGGIRLITEALEDYLPGFYRGKLVYLSGPSHAIEVAEGKIT
                     GLISASRSGKNSIRVRELLSGGRLVVFSSLDVRGVQISAALKNVVAIAFGILDALKET
                     SDQFGDNTESLLMAAGLNEIQVIGRAMGATHPETFTSIAGVGDLDVTCRSLHGRNRRF
                     GREIVLKGLLADCASIAEVISSLPRFGYLAEGAVTARWAQTIAVQKRLELPILGGVYR
                     ILNRDVEPIAEVSNMIERILSRPGSGGRRHGATSILRGTAGWASRLARRG"
     gene            complement(4565..5863)
                     /locus_tag="VFH83_11815"
     CDS             complement(4565..5863)
                     /locus_tag="VFH83_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487101.1"
                     /translation="MGKAYGRLLLVAACFLAAQLLGAQARTGAQGYPELTPLPLSSQL
                     SAMREPLPVETIVDAALEFSGASDDLASADKDKLSGLIMRFRAEVADVTSPADLADRA
                     LAFLHKNVFTRYSVRQARVDTALESGVYNCVSSAVIYLILARSVGLSVGGVRTTDHAF
                     DTVLINGQPVDVETTNPYGYNPGSRKEFTDSFGKVTGYSYVPPSNYADRRAIGEKELL
                     SLILYDRVSEYVDGKYLRDALQPAVSAYTLIGSDELRLILINLLGAYSASFEGRKDWP
                     GAIQFLDSAKASLGALPELEKRRREVYHNWVVTLIASNALTDAEAMLEQPAVQASLED
                     ADWANLSIATVQALADQEARANGYAAAASFVVDAIHRIGARPLLLQTYEVYAHNTFAL
                     LFNQRKYSEAKTTVDQALQIYPDSHTLSQDLDLVKRALKQ"
     gene            5968..7074
                     /gene="ald"
                     /locus_tag="VFH83_11820"
     CDS             5968..7074
                     /gene="ald"
                     /locus_tag="VFH83_11820"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET6487102.1"
                     /translation="MRIAVPKEIKKHEYRVGMTPHSVRAYIKHGHEVLVEKGAGVGSG
                     YEDGEYQAAGARITDDKKELFRQSEMIVKVKEPLSDEYALFYKDQILFTYLHLAANLE
                     LAQELLKRGVSGVAYETIELSDRSLPCLTPKSEIAGRLSVQEGAKYLEKEFGGRGILL
                     GGVPGVQRGRIGILGGGVVGTNACKIAVGIGANVTVLDINAKRLAYLDDIFGSSITTL
                     YATEANIESVLRESDIVIGAVLVAGETAPRLVTKQHLKIMQPGAVIVDVAIDQGGCTE
                     TSRPTTHDDPIYIVDGVVHYCVANMPGAVARSSTIALTSVTLPYGLMIADSGLRAATR
                     TSEALRRGLNTIGGTCVHPGVAKSCNLPYTEYRA"
     gene            7156..>7484
                     /locus_tag="VFH83_11825"
     CDS             7156..>7484
                     /locus_tag="VFH83_11825"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET6487103.1"
                     /translation="MGQHYLGFRSHYSLLRGCRSPEEICRFAREQGISTVGLTDLNGF
                     YGLIRFLVAAQREAVKPVVGVTVERGGSQVFTLYVLNRNGFSRACRLLTELFTADDTF
                     DPVTYLE"
BASE COUNT         1352 a         2420 c         2452 g         1260 t
ORIGIN      
        1 gggttcaacg tggacctgga gtctctcacc gtcccgggga ccagcaccac gcacgtgaac
       61 ctgtacgatg gaaccgtgga gggcctggag aaccggtccc tgcggctctt cagcgtccag
      121 taccatccgg aggcgtgccc agggccccat gaggcccgtt acctcttcga tcgattcgtg
      181 gccatgatgg acgagggacc gagggacgtt tcaacggcaa cgccgggcct cggcgttccc
      241 gtaccccggc gtcccgtgta ggacggtaac catgccccgc cgaacggaca tccattcgat
      301 cctgatcatc ggcaccggac ccattgtgat cggccaggcg tgcgagttcg actactcggg
      361 cacgcaggcg tgcaaggcgc tgcgggaaga gggcttccgc gtggtgctcg tcaactccaa
      421 ccccgccacc atcatgacgg accccgagat ggcggatgca acctatatcg agccgttgac
      481 cccggcgatg gtggagaagg tgatcgaggc cgaaaagccg gacgcgctcc tgcccacgct
      541 gggaggacag acggggctca acctggccat ccagctcgcg gactccggtg tgctggaccg
      601 gctgggcgtc cgactgatcg gcgcggaccg acaggccatc cgaaaggcgg aggaccgaag
      661 cctcttcaag gaatccatgc agaggatcgg gatcgacctt ccccgaagcg gcctggcgca
      721 cacgccggag gaagcgcggc gcgttgcctc ggagatcggg tttcccctga tcatccgacc
      781 gtccttcacc ctcgggggga cgggcggcgg catcgtgccg gacccggcgg ccttcgacga
      841 gatggctcgc cgaggccttt ccgccagcat ggtctccgag atcctgatcg aggagtccat
      901 tgccggctgg aaggaatacg agctggaggt catgcgagat cgggcagaca acgtggtgat
      961 cgtctgctcc atcgagaacc tggaccccat gggcgtccac acgggggact caatcacggt
     1021 cgccccggct cagacgctca cggaccggga ataccagacc atgcgggacg cggccatcgc
     1081 gtgcatccgg gagatcgggg tggataccgg cggctccaac atccagttcg ccgtcaaccc
     1141 ggagaacggc cgtatggtcg tcatcgagat gaacccgcgg gtctcgcgat cctccgcgct
     1201 cgcctccaag gcgaccggat tccccatcgc caagatcgcc gccaagctgg ccgtgggtta
     1261 cacgctggaa gagatcccca acgacatcac ccgaagcacc ccggcctcct acgagccctc
     1321 catcgactac tgcgtggtca agataccgcg cttcgccttc gagaagttcc cggccgccga
     1381 ccccaccctg accatccaga tgaagtcggt cggagaggtg atggccatcg gccgcacgtt
     1441 caaggaggcg ctccagaagg ccattcgagg gctggagatc cggcggcccg gtctcagtgg
     1501 cgtagggaac gtcgccgagg ggcttctcac cgagatgctc gcgcggccca acgccgaacg
     1561 gctgtactac ctgtacgacg cgctccagcg cggctggtcg gtggagcggg tggcgtcgct
     1621 gacccacatc gacccgtggt tcctggaaaa cgtgcgccag atcatcgagt tcgagcccac
     1681 catctccacg gccaacctgc gggaggccaa gcagatgggg ttcagcgacg cgcagatcgc
     1741 gcgccggctc tccacgaacg aggcggccat ccggggcgag cgccagcgcc aggggatccg
     1801 tccggcgtac aagctggtgg acacctgcgc ggcggagttc gaggcgtaca cgccttacta
     1861 ctactcgacc tacgacccgg aggacgagac ccgcgcctcc aatcgaaaga agttcctgat
     1921 cctgggcggc ggcccgaatc ggatcggaca ggggatcgag ttcgattact gctgcgtcca
     1981 tgccgccttc gcccttcggg agatcggtta tgagaccatc atggtcaact ccaatccgga
     2041 aacggtctcc acggattacg atacctcgga caagctgtac ttcgagccgc tgaccatgga
     2101 ggacgtgctg gccatcgtgg accgggagaa gccgcagggc gtgatcgtgc agctcggagg
     2161 ccagacgccc ctcaatctcg ccgtggggct ggcccaggcc ggtgtgccca ttgtcggcac
     2221 cagccccgat gccatcgacc gggccgaaga ccgccggcgc ttctccgagc tcgtccagaa
     2281 gctcgggctt cggcagccgg agaacggctc cgcggtcaag gtggacgagg cgcggggaat
     2341 agcccgggag atcggctacc cggtgctggt ccgccccagc tacgtcctgg gcggccgggc
     2401 catggagatc gtctgggacg atgcatccct ggagcactac atgcagcggg cggtctgggc
     2461 atctcccgaa cgacccgtgc tcatcgaccg gttcatcgag gatgccatcg aggtggacgt
     2521 ggacatggtg agcgacggcc gccgctcggt gatcggcggg atcatggagc acatcgagga
     2581 agcgggcgtc cactcggggg acgcggccat ggtgcttccg cctcacaccc ttccgcactg
     2641 gatgctggaa gagatccgcc gctacacgcg ccttcttgcg gaggagctca cggtggtcgg
     2701 actcatgaac gtgcagtacg ccgtgcgagg caaggagctg tacatcatcg aggtgaaccc
     2761 tcgggcgtcc cgaaccgtgc cgttcgtgtc gaaggccacg ggtgtccccc tggccaagat
     2821 cgcggccaag gtcatggcgg gcatgaccct ggaggaggcc gggttcaccg acgaggtgat
     2881 cccccggcac gtctcggtca aggagtccgt gcttccgttc gcccggttcc ccggcgtgga
     2941 cgtggtcctg ggaccggaga tgaagtccac gggtgaggtg atggggatcg atcgggactt
     3001 tggccgcgcc tactgcaaga gccagctggc ggcgctgcag agggttcctt cggagggcgc
     3061 cgtcttcatc agcgtgaagg accgggacaa gacgcagctc atcgtttccg tcgcctacca
     3121 gctcgtctcc gtggggttca cgatcgtgtc cacctccgga accgctgcct tcctcaagga
     3181 aaacgggata ccggcggagg tggtccgcag ggtcagcgag ggcgccccca gcgtcctgga
     3241 cctgatcaac gagggcaaga tccagctcat catcaacacg cctaccggca agatcccccg
     3301 ccgcgacgag gtcagcatcc gcgccatcgc aaccagccgc gggatcccgg tggtcaccac
     3361 catgcccggg gcagatgcct atgcccaggg gatccgggcg ttcatccgcg gcgagctagg
     3421 cgtgaggccc atccagcggt accacgcaga attgaagtag ccccgtgccg gcggccaccg
     3481 ctgccgggcc ggctcaggat acgctcgatc atgttgctca cctcggcgat gggctccacg
     3541 tcccggttca ggatgcggta gaccccaccc aggatcggca gctccagccg tttctggact
     3601 gcgatagtct gcgcccagcg tgccgtgacc gccccctcgg caaggtagcc gaatcgaggg
     3661 aggctggaga tcacctccgc gatggaggcg caatcggcca gaagcccctt gaggacgatc
     3721 tcccgcccga agcgccggtt cctcccgtgg agactcctgc aggtgacgtc cagatcccct
     3781 acgccggcaa tggaggtgaa cgtctcgggg tgggtggctc ccatggcccg gccgatcacc
     3841 tggatctcgt tcaggccggc cgccatcagc agactctccg tgttgtctcc gaactggtcc
     3901 gaggtctcct tgagagcgtc caggatcccg aaagcgatgg ccacgacgtt cttcagcgcc
     3961 gcgctgatct ggaccccgcg gacgtccagg gaagagaaca cgaccagccg gcctccggag
     4021 aggagctccc ggacgcggat ggagttcttt ccgcttcgcg acgcgctgat gagcccggtg
     4081 atcttgcctt ccgcaacttc gatggcatgg ctggggccgg agaggtatac cagcttcccg
     4141 cggtagaacc cgggcaggta gtcctcaagg gcttccgtga tgaggcggat cccgcccggg
     4201 gtctccagga atcccttggt gaggatggag accgtggtcc tgccttcccg gatgtccgcg
     4261 cacgagatga ccttcttcgc gttttccagc aggtacaacg acgggatggc gatgaagagg
     4321 aagtccttgt cctccgcaac actcagcaga ttcgagcagg cgcgaagacc ttccggcagc
     4381 gaaacgccgg gcaggtagcg gctgttggag cgccgctggt tgatctcctc gaccaggtcc
     4441 tcctcaaagg tccagagctc ggtgtccagg cctttctccg cgaggacctt ggcgatggtg
     4501 gttccccacg ctccggcgcc caacaccccg atcctggtgc ccgtccccgt gaccatgcgc
     4561 gccactactg cttgagcgcc cgcttcacca gatcgagatc ctgcgagagg gtgtggctgt
     4621 cggggtagat ctggagcgcc tgatccaccg tggtcttggc ctcggagtac ttgcgctggt
     4681 tgaaaagcag cgcgaaggtg ttgtgggcgt agacctcata ggtctggagc agcaggggcc
     4741 gcgctccgat gcggtggatc gcgtccacga cgaacgatgc cgcggccgcg tagccgttgg
     4801 cgcgcgcctc ctggtccgcc agggcctgga cggtggcgat ggagaggttt gcccagtcgg
     4861 catcttccag ggaggcctgc acggccggct gctccagcat ggcttcggca tccgtcagcg
     4921 cgttggatgc gatcagcgtg accacccagt tgtggtagac ctcccgtcgg cgcttctcca
     4981 gctctggcag cgcccccagg gaggcctttg ccgaatccag gaactggatg gcgcccggcc
     5041 agtccttgcg gccttcgaag gatgcggagt aggcgcccag caggttgatc aggatcaagc
     5101 gcagctcgtc gctgccgatc agggtatagg cgctcacggc gggctgcaag gcgtcccgaa
     5161 ggtattttcc gtccacgtac tcggagaccc ggtcgtacag gatcaggctg agaagctcct
     5221 tctcaccgat ggcgcggcgg tccgcatagt tgcttggcgg cacatagcta tacccggtca
     5281 ccttgccgaa ggaatcggta aactcctttc gggagccagg gttgtagccg taggggttcg
     5341 tggtctcgac gtccaccggt tggccgttga tcaggaccgt gtcgaaggcg tggtcggtag
     5401 tcctgactcc acccacggac aggcccacgg acctcgccag gatgaggtaa atcaccgccg
     5461 aggaaacgca gttgtagacg ccgctctcca gcgccgtgtc cacccgcgcc tggcgcaccg
     5521 agtagcgcgt gaacacgttc ttgtggagga acgccagcgc ccggtccgcc agatccgccg
     5581 gggaggtcac gtccgccacc tcggcccgaa accgcatgat gaggccggag agcttgtcct
     5641 tgtccgcgga ggccagatcg tcggaggcgc cggagaactc cagcgccgcg tccacgatgg
     5701 tctcgaccgg caacggctcg cgcatcgccg agagctggga gctcaaggga agaggagtga
     5761 gctcgggata cccctgggcg ccggtccggg cctgggcgcc gagaagctgc gcggcgagga
     5821 agcacgccgc cacgaggagc agccgtccgt atgctttacc catcagacca tccacccaca
     5881 agatgagctt tcctctggaa tctggtcaag ggttgcggta cagtgcgctt cgagaccttt
     5941 cttttccact aacccaggag gattcgcatg aggatcgccg ttcccaagga gatcaagaag
     6001 cacgagtacc gcgtggggat gaccccccat tccgttcggg cctacatcaa gcacgggcac
     6061 gaggtactgg tagagaaggg cgccggggtc ggctcgggat acgaagatgg cgagtatcag
     6121 gccgcgggag cccggatcac cgacgacaag aaggaattgt tccgacagtc cgagatgatc
     6181 gtgaaggtca aggagcctct gagcgacgag tacgccctct tctacaagga ccagatcctg
     6241 ttcacgtacc ttcaccttgc cgcaaacctt gagctggccc aggagctgct gaagcgaggg
     6301 gtctccggcg tggcgtacga gaccatcgag ctttcggacc ggtcgcttcc ctgcctcacc
     6361 cccaagagcg agatcgcggg ccggctctcc gtccaggagg gcgccaagta cctggagaag
     6421 gagttcgggg ggcggggcat cctgctgggc ggagtccccg gagtccagcg cggcaggatc
     6481 ggcatcctgg gtggaggcgt ggtcggaacg aacgcctgca agatcgccgt ggggatcggc
     6541 gccaacgtga ccgtgctcga catcaacgcc aagaggctcg cctatctcga cgacatcttc
     6601 ggcagctcca tcaccaccct ctacgccacg gaggcgaaca tcgaatccgt gctgcgggaa
     6661 tccgacatcg tgatcggcgc ggtcctggtg gcgggagaga cggccccgag gctcgtgacg
     6721 aaacagcacc tcaagatcat gcagcccggg gcggttatcg tcgatgtggc gatcgaccag
     6781 gggggatgca cggagacctc gcggccgacg acccacgacg atcccatcta catcgtagac
     6841 ggagtcgtcc actactgcgt ggccaatatg cccggggcgg tggcccggtc ttcgaccatt
     6901 gcgctcacga gcgtcaccct tccgtacggg ctcatgatcg cggacagcgg actgcgcgcc
     6961 gccacccgga cgagcgaggc gcttcgccgc gggctgaaca ccattggagg aacctgcgtc
     7021 cacccggggg ttgccaagag ctgcaacctt ccttacacgg agtaccgggc gtagaatccg
     7081 ggccgggtgt ctcgacccgc cctcccaaaa gacggcttgc gcttgaggcc catatactat
     7141 acatttgtat agtatatggg ccagcattac ctgggcttcc gctcccacta ttccctgctc
     7201 cgagggtgcc gatctccaga ggagatctgt cgtttcgcgc gggagcaggg catctctacg
     7261 gtcggactga ccgatctgaa tggcttctac ggcctcatcc gcttcctcgt tgcggcccag
     7321 cgggaggcgg tcaaacccgt agtcggcgtg acggtggaac gcggcggctc tcaggttttc
     7381 actctgtacg tcctgaaccg caacggtttc agccgggcct gtcgtctcct gacggagctc
     7441 ttcaccgcag atgacacctt tgatcccgtg acgtacctgg agga
//
LOCUS       DASRZS010000484         3513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3333428, whole genome shotgun sequence.
ACCESSION   DASRZS010000484 DASRZS010000000
VERSION     DASRZS010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3333428"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /gene="rlmN"
                     /locus_tag="VFH83_11830"
     CDS             <1..979
                     /gene="rlmN"
                     /locus_tag="VFH83_11830"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET6487104.1"
                     /translation="GLPAYGARQVADWMYGKDSRDFDRMTNLPRAARRALSEAYTIER
                     RDPSRVTESSDGTRKYLFPAPHGGFVEAAWIPEEDRSTLCLSVQVGCKMGCLFCMTGK
                     QGFQGHLDSGEILNQFASLPERERITNIVYMGMGEPLDNLEPVLASLDVLCSSYGYGL
                     SPTRITVSTVGIIPGMKEFLARSRCHLAVSLHSPFEEERRKLMPVEHVYPLNEVLAVL
                     REEAVRGQRRISFEYILWEGLNDTPRHSRELARILNGIRCRVNLIPFHPIPGTPLKPS
                     PRESMERFETDLRRYGITTTIRQSRGQDIAAACGMLSTRELVRQRAESE"
     gene            complement(999..1748)
                     /locus_tag="VFH83_11835"
     CDS             complement(999..1748)
                     /locus_tag="VFH83_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HET6487105.1"
                     /translation="MLYTLSLVAILVAAPVVFIALYFVTAPYGRHFRDGWGPSMPARF
                     GWMAMEAPALVVMAAAVLYHASSVSLLAFALLALWEIHYVYRTLVFPFLIRERQTRMP
                     VVLAVMAFVFNCLNGYANGTWLAGFPRDGDGGAIFTIRVAVGAALFACGFVTHVLSDR
                     TLRSLRRNGDGGYSIPHGGLFEFVSSPNYFGEILEWCGWAIATWSLPGLAFALFTVGN
                     LVPRAHANRRWYQATFPEYPAQRRRVIPFVY"
     gene            1873..2559
                     /locus_tag="VFH83_11840"
     CDS             1873..2559
                     /locus_tag="VFH83_11840"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET6487106.1"
                     /translation="MTRRMKGRLVDLSHRLKPGKEEYHLDLETHFTDDLYPQYKRSPD
                     TWYILQDVSMSSHCGTHIEFPYHHCKTGMDAASFPLERLVGDCQLLDFRHKQPGDMVG
                     LQDLEPHASSIQKGDMLLFNFGCARFYRTPRSHDRPQVSNEAIRWLVLDRGISLIGSD
                     ASGIELKGVADQPNHQFLMDHGVPIIEAAANLDELRTRRFTLLVLALSAEGLDSCPVR
                     LVALEDEVCG"
     gene            2543..>3513
                     /locus_tag="VFH83_11845"
     CDS             2543..>3513
                     /locus_tag="VFH83_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004069554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET6487107.1"
                     /translation="MKSVDKGKALGLYRTMLAIRRFEEKLYFLFSTRDMPGSMHQYNG
                     QEAVAAGVCAHLAATDYITSTHRGHGHCIAKGADLNAVMAEMFAKSTGCCKGMGGSMH
                     IADFSVGMLGANGIVAAGLPIAVGAALTCQNRGSGVSVAFFGDGGANEGAFHEAVNLA
                     AVWSLPVVFVCENNLYGFSTHVRRTMSIERISQRAGAYGIPGVSVDGMDVLAMYDAAG
                     EAVDRARSGRGPTLLEALTYRFMGHSRFEPSSYRTKEELEEWKRRDPIVQLRSALVTR
                     FKAAEEEIARVEQAVDAEIEESVRFAESSPDPAADDWRKYLYAGEAH"
BASE COUNT          661 a         1147 c         1157 g          548 t
ORIGIN      
        1 gggccttccg gcctatggcg cgcgccaggt tgccgactgg atgtacggca aggactcccg
       61 cgatttcgac cgcatgacga acctgccccg cgccgcccgc cgcgcgctct cggaggccta
      121 caccatcgag cggcgggacc cgtcccgggt gaccgagtcc tccgacggca cgcgcaagta
      181 tctcttcccc gcgcctcacg gaggcttcgt ggaagcggcg tggatacccg aagaggaccg
      241 cagcacgctc tgcctctccg tccaggtggg ctgcaagatg ggatgtctct tctgcatgac
      301 gggcaaacag gggttccagg ggcacctgga cagcggagag atcctcaacc agttcgcgag
      361 ccttccggag cgcgagcgga tcaccaacat cgtctatatg ggcatggggg agccgctgga
      421 caacctcgag ccggttctgg ccagcctgga cgtgctctgc tccagctacg ggtacggcct
      481 cagccccacc aggatcaccg tatccacggt gggcatcatc ccgggcatga aggagttcct
      541 tgcccggtcc cgctgccacc ttgccgtgag cttgcactca ccgttcgagg aggagaggcg
      601 gaagctgatg cccgtggagc acgtctatcc cctcaacgag gtgctcgcgg tcctgcgaga
      661 ggaagcggtc cgcgggcagc ggcggatctc gttcgagtat atcctctggg aaggtctgaa
      721 cgacacgccc cgccattcgc gagagctggc gcggatcctg aacgggatcc ggtgccgcgt
      781 gaacctgatc ccgttccacc ccattcccgg caccccgctg aagccgagtc ccagggagtc
      841 catggagcgc ttcgaaacgg acctgaggcg ctatgggatc acgaccacca tccgccagtc
      901 acggggacag gatatcgccg ccgcgtgcgg gatgctctcc acccgggagc tggtccgcca
      961 acgcgcggaa tcggaataga gcggcctgga gacccccctc agtacacgaa cggtatcacg
     1021 cgtctcctct gggcggggta ctcggggaac gtagcctggt accaccggcg gttggcgtgc
     1081 gcccgcggga cgaggttgcc cacggtaaag agcgcgaacg ccaggcccgg cagcgaccag
     1141 gtggcgattg cccagccaca ccactccagg atctctccga agtagttcgg gctggagacg
     1201 aactcgaaca gcccgccgtg ggggatgctg tagccgccgt cgccgtttcg ccgcagggag
     1261 cgtagagtcc ggtcggagag cacgtgggtc acaaagccac aggcgaacag ggccgctccg
     1321 accgccacgc ggatcgtgaa gattgcgccg ccgtccccat cccgcggaaa gcccgccagc
     1381 cacgtgccgt tggcgtagcc gttcagacag ttgaacacga acgccatcac ggccagcacg
     1441 accggcatgc gcgtctgccg ctctcggatg aggaacggga agacaagcgt ccgatagaca
     1501 tagtgaattt cccagagggc cagcagcgcg aatgccagca ggctgaccga ggaggcatgg
     1561 tagagcacgg ccgccgccat gacgaccagg gccggcgcct ccattgccat ccagccgaag
     1621 cgggcgggca tggacgggcc ccagccgtcg cggaaatgcc tgccataggg ggccgtgacg
     1681 aagtacaggg cgatgaacac gaccggcgcc gccaccagga tggccacgag agacagcgtg
     1741 taaagcatag gcatcccggg tactttagca ggctatgccc ctggtgacaa tcgccaagtc
     1801 ttgacagccg gatgaaacaa agtttacagt cctccgaaag aactcgtaaa tactttcaag
     1861 gccggagcaa ccatgacgcg taggatgaaa gggcggctgg tggacctgag ccatcgcctg
     1921 aaaccgggga aagaggaata ccacctcgat ctcgaaacgc acttcacgga cgacctctat
     1981 ccccagtaca agcgcagccc ggacacctgg tacatccttc aagatgtcag catgtcctcg
     2041 cactgcggca cgcacatcga gtttccgtac catcactgca agaccggaat ggatgcggcc
     2101 agcttccccc tggagcggct ggtgggggat tgccagctcc tggacttccg tcacaagcag
     2161 cccggagaca tggtcggcct ccaggacctg gagccgcacg catcttcgat ccagaagggc
     2221 gacatgctgc tcttcaactt cggctgcgcg cggttctacc gaacgcctcg ttcccatgac
     2281 cgtccccagg tctccaacga ggccatccgt tggctggtcc tggaccgggg gatctcgctg
     2341 atcggctccg acgcctccgg catcgagctg aagggcgttg cggaccagcc caaccaccag
     2401 ttcctcatgg atcacggcgt gccgatcatc gaggcagcgg cgaacctgga tgagcttcgg
     2461 acccgtcggt tcacgctcct cgtgctggcg ctgagcgcgg agggtctgga ctcctgcccc
     2521 gtacggctcg tggcgctgga ggatgaagtc tgtggataag ggcaaggcgc ttggactgta
     2581 tcggaccatg ctggcgatcc gcaggttcga ggagaaactg tacttcctct tctccacgcg
     2641 ggacatgccc ggcagcatgc accagtacaa cgggcaggag gcggtggccg ccggagtctg
     2701 cgcccacctg gccgcaacgg actacatcac cagcacgcac cggggccacg gacactgcat
     2761 cgccaagggc gcggacctca acgccgtgat ggccgagatg ttcgccaagt ccaccggctg
     2821 ctgcaagggc atgggcggct ccatgcacat cgccgacttc agcgtgggca tgctgggcgc
     2881 caacggcatc gtggcggccg ggctccccat cgccgtgggg gcggctctca cctgccagaa
     2941 ccgcggcagc ggcgtgtcgg tggcgttctt cggtgacggc ggggcgaacg agggcgcctt
     3001 ccacgaggcc gtgaacctgg ccgccgtctg gtcgttgccg gtcgtcttcg tctgtgagaa
     3061 caacctctac gggttctcga cgcacgtccg gcgcaccatg tccatcgagc ggatctccca
     3121 acgcgcgggc gcgtatggaa tccccggcgt ctccgtggac gggatggacg tcctggcgat
     3181 gtacgatgcg gcgggggagg cggtcgatcg ggcgcgctcg ggccgcgggc caacgctcct
     3241 ggaggcgctg acctaccgat tcatggggca ctcccggttc gagccgtcca gctaccgcac
     3301 caaggaggag ctggaggagt ggaagcgccg ggaccccatc gtgcagctcc gctccgcgct
     3361 ggtcacgcgg ttcaaagccg ccgaggaaga gatcgccaga gtggaacaag cggtggacgc
     3421 ggagatcgag gagtcggtac ggttcgcgga atcgagcccc gatcccgcgg cggatgattg
     3481 gcggaagtac ctctacgccg gggaggcgca ttg
//
LOCUS       DASRZS010000485         3094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2573115, whole genome shotgun sequence.
ACCESSION   DASRZS010000485 DASRZS010000000
VERSION     DASRZS010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2573115"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="VFH83_11850"
     CDS             complement(<1..1180)
                     /locus_tag="VFH83_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6487108.1"
                     /translation="MLTAYSLSELTVVTPQTAIPSSAIHVEHRKIGRMGGKSKMDVRV
                     GDGVAAYPALINVHDHFNGNYLPKVGPPPGEYYLNWSYWEKDLRSSEVVRNERSKTSA
                     EERYFLSAYKNLFSGVATAHDHFPHEWNEPFIPRLPIRTLRNYALAHECSSFDLKWGD
                     GIEVEHARARKSNLPFVTHLEEGFDEETQRGVDILESLGCLDDHDVLIHCVGFSDEDI
                     QKTRRAGASIGWCPASNLFMYNLTMKVRKMLEAGITVAIGTDSTATGSVNLLEEMRFG
                     RQLYQRMYGEDLPAKTVVNMVTTNPARAFRMQKEIGSLEEGKLADLLVVRQKAEDPWE
                     SLVNARPQDIELLTLDGSPILGDCRFEELFSGRGVPYTKVRLEGRQMLVKGDPVGLME
                     R"
     gene            complement(1259..2299)
                     /locus_tag="VFH83_11855"
     CDS             complement(1259..2299)
                     /locus_tag="VFH83_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6487109.1"
                     /translation="MGRHLEKGRAGTPSRFDEEAHEHGGYPALIEVPRRGDVSDERDL
                     LALYIKQIARYPLLTAQDEQEIGEAIQIAREELEGLEKKAAACRVPTRSYLRRKAELE
                     GHVTRMKNRLILGNLRLVVSVAKKYQHRGVSLLDLVDEGNIGLIEAVERFDYRKGCRF
                     STYGTWWIKQAVIKALAENGKAIKIPIHLLNTIKKCFSIARQLTQEYGREPTNAEIAD
                     YLGMTKTRVEELISLSWDPTSLDTTVAGENPSRLSDLIRDDASAKPYESAFAMALQDT
                     LAMVLDKLNQREIRIIKLRFGLDGEGPFTLEETGRILGITRERVRQIQERALVKLKQL
                     RAADQLEAFIQG"
     gene            complement(2310..>3094)
                     /locus_tag="VFH83_11860"
     CDS             complement(2310..>3094)
                     /locus_tag="VFH83_11860"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET6487110.1"
                     /translation="QVPLDVLRQFNGFPQAIKLKVGQLVRFPDVYTVRKGDTLYGIAR
                     DLSVPLSAVLDLNGLTQGSRIVPGQKLFVPGTAKAVQAPADGASTPASGSQPSGTQSG
                     SPQPGGQPAGGQDPSAKPPDLGPSSGAGQLATAAGGAFVWPHPGRREPYTGRLPGLLF
                     HGAKGDLVQSATAGEVEWVAPYLGYGIAIIIRAPDKSLLTYKGNESALVSVGDRVIPG
                     TEIARLGISPLLGGDARLYLSIKDSKGQTVDPEKYLSIKNQA"
BASE COUNT          488 a         1021 c          973 g          612 t
ORIGIN      
        1 cccgctccat cagccccacg gggtcccctt tgaccagcat ctgcctgccc tccagacgga
       61 ccttggtgta cggcactccc cgtccgctga agagctcctc gaaacgacaa tcccccagga
      121 tcggcgaccc gtccagcgtg agaagctcga tgtcctgggg ccgcgcgttg accagggact
      181 cccaagggtc ctccgccttc tgacggacca cgaggagatc cgcgagcttg ccctcctcca
      241 ggcttccgat ctctttctgc atccggaagg cgcgcgccgg attggtggtt accatgttga
      301 cgaccgtctt ggcagggaga tcctctccgt acatccgctg atagagctgc cgaccgaacc
      361 gcatctcctc cagcaggttc acggatccgg ttgccgtgga gtccgtcccg atggccaccg
      421 ttatcccggc ctccagcatc tttcggacct tcatggtcag gttgtacatg aagaggttgg
      481 acgccgggca ccaaccgatg ctggcgccgg ccctccgggt cttctggatg tcctcatccg
      541 agaacccgac gcagtggatg agcacgtcgt ggtcgtccag acagcccagg gattccagga
      601 tgtccacgcc ccgctgggtc tcctcgtcga agccctcctc caggtgcgtg acaaagggca
      661 ggttgctctt gcgggcgcgc gcgtgctcga cctcgatccc gtcgccccat ttcaggtcga
      721 aagaggagca ctcgtgcgcc agcgcgtagt tgcggagcgt tcggatggga agccgcggga
      781 tgaacggctc gttccactcg tgggggaaat ggtcgtgggc ggtcgccacc ccggagaaga
      841 ggttcttgta cgcgctcaag aagtagcgct cctccgcgct ggtcttggag cgctcgttgc
      901 gcaccacctc ggagctccgc aggtccttct cccagtacga ccagttgagg tagtactccc
      961 cgggcggggg gcccaccttg gggaggtagt tgccgttgaa gtggtcatgc acgttgatga
     1021 gcgcgggata ggcagcgact ccgtccccta cccgcacgtc catcttggac ttgccgccca
     1081 tgcggccgat cttgcggtgc tccacgtgaa tggcgctcga agggatggcg gtctggggag
     1141 tcacgacggt caactcgctc agggagtacg ctgtgagcat tcatcttcct tcgtcgtatc
     1201 cccattgtag gagcgggtca ggggcatgtc aagtttctcg cgccgttcga cggcccgctc
     1261 atccctggat gaacgcttcc aactgatccg cggcgcgaag ctgcttgagc ttgaccaggg
     1321 cgcgttcctg gatctgccgc acgcgctccc gggtgatccc gaggatccgt ccggtttcct
     1381 ccagggtgaa cgggccctcg ccgtccagac cgaaccgcag tttgatgatc cggatctccc
     1441 gttggttgag cttgtccagc accatggcca gggtatcctg cagggccatg gcgaacgccg
     1501 actcgtaggg tttcgcggag gcgtcgtccc gaatgaggtc cgagagcctg gatgggttct
     1561 caccggcaac cgtggtgtcc agcgaggtgg gatcccacga gaggctgatc agctcctcca
     1621 cccgcgtctt ggtcatcccg aggtagtcgg cgatttcggc gttggtcggc tcgcgcccgt
     1681 actcctgggt aagctgacgc gcgatcgaaa aacacttctt gatggtgttg agcaggtgga
     1741 tcgggatctt gatggccttg ccgttctccg caagcgcctt gatgaccgcc tgcttgatcc
     1801 accacgtgcc gtaggttgag aatcggcatc ccttgcggta atcgaaacgc tcgaccgcct
     1861 cgatcaggcc gatgttcccc tcatccacca ggtccaggag ggagacgccc cggtgctggt
     1921 acttcttggc cacggagacc acgagccgga ggttccccag gatcaggcgg ttcttcatgc
     1981 gggtgacgtg cccttccagc tcggccttcc gacgcaggta ggacctcgtc gggacccggc
     2041 aggcggccgc cttcttctcc agcccttcga gctcctctcg cgcaatctgg atggcttccc
     2101 cgatctcctg ctcgtcctgg gccgtgagca gaggatagcg ggcaatctgc ttgatgtaga
     2161 gcgcgagcag gtccctctcg tcggatacat ccccgcgtcg tggaacttcg atcagcgcgg
     2221 gatagccacc gtgctcgtgc gcttcctcgt cgaaccgcga cggggtccct gctctgcctt
     2281 tctcgaggtg ccgtcccata tacaccctct cacgcctggt tcttgatgga gagatacttc
     2341 tccggatcga ccgtctgccc cttggagtcc ttgatgctga ggtacagcct cgcgtctcca
     2401 ccgagcaggg ggctgatccc cagtcgggcg atctccgtcc ctggaatcac ccgatctccg
     2461 acgctcacca gcgccgactc gttgcccttg tacgtcagga ggctcttgtc cggcgctcgg
     2521 atgatgatgg cgatgccgta ccccaggtac ggcgccaccc actccacctc tcccgcggta
     2581 gccgactgta cgagatcccc cttcgcgccg tgaaagagga gccccggtag acggccggtg
     2641 tatggttcgc gccggccggg atgcggccag acgaacgcgc cgccggcggc ggtggcaagc
     2701 tgccccgctc ccgacgatgg cccgagatcg ggcggcttgg cggaggggtc ctgacctccg
     2761 gcgggctggc caccgggctg cgggctgcca ctctgggttc cggagggctg gctcccagaa
     2821 gccggcgtac tggcgccgtc ggccggcgcc tgcacagctt tggcggtgcc agggacgaag
     2881 agcttctggc cgggaacgat ccgcgagccc tgggtcaggc cattcaagtc gagaacggcc
     2941 gacaggggca ccgagaggtc tctggcgatc ccgtagagcg tgtcgccctt tctcacggta
     3001 tatacgtcag gaaaccgcac caactgaccg accttcagtt tgatggcctg ggggaacccg
     3061 ttgaactgcc tgagaacgtc caggggcacc tgct
//
LOCUS       DASRZS010000486         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5652058, whole genome shotgun sequence.
ACCESSION   DASRZS010000486 DASRZS010000000
VERSION     DASRZS010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5652058"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VFH83_11865"
     CDS             complement(<1..836)
                     /locus_tag="VFH83_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HET6487111.1"
                     /translation="MSGKRRRSSPVRAAAALLALLLSAPPLAADVFTYAGDRMTTVLA
                     EGAQHALLTGHAEVATTDMRIDADQIELFGKDFIYAQCRGDVKVVDAKRGLDLSSTQL
                     FYDRQQKIARVQGNAMMQDLKNEMVVKGGFIEDRDKEQLTIIQIGVRILKKDLVCRAE
                     YAKYYRDKKILELSGMPWVSRKGDIFQAARITVNLDTDEITLDSGVQGSIQESTDQGT
                     SPEQNGQQPAGAQPAGAPSSGAQPSGSQPEQPGQPAGSGQTGQDQSSGSSSSQSQKPP
                     QSG"
     gene            complement(811..1398)
                     /gene="lptC"
                     /locus_tag="VFH83_11870"
     CDS             complement(811..1398)
                     /gene="lptC"
                     /locus_tag="VFH83_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HET6487112.1"
                     /translation="MSEEPGTAKLRRALVGAVLVLSLLCAGCSLDYGDQTVVEKQPEN
                     IPDTVAVGLVHRVNKGGKVSVQLEASRAESFSSTSTTVLTDAHFTEYDDKGAMDTEGR
                     ASHVVFHTDSQDAEISGAVQVYSAREKANIRAESLSWVDKTKRLTADPDERVLLKKDD
                     GSFITGSGFLGDFRHRQIVFSGPVEGQYVWQEKKE"
     gene            complement(1368..2981)
                     /locus_tag="VFH83_11875"
     CDS             complement(1368..2981)
                     /locus_tag="VFH83_11875"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET6487113.1"
                     /translation="MSKYIFVTGGVCSGLGKGVASASIGSLLENRGFSIRMIKIDPYL
                     NVDAGTMNPFQHGEVYVTDDGAETDLDLGNYSRFTSSPIGRDNSITTGQVYQAVITKE
                     REGRFLGRTVQVIPHITDEIKARIFRVGEQAGVDVTLVEVGGTVGDIESVPFLEAARQ
                     FIHDLGKENVLFVHLTLVPQVYWGEPKSKPTQHSVRELREIGIQPDILLCRSVSRLDE
                     DLRRKIALFTNVEKEAVISAFDVRTTIYEIPLTFVEQKVDALIIKKLGLAEEAPIRDG
                     WQKVVDHFTEAKRTVRVGIVGKYTGLHDSYISIFESLFHGGIANASRVQLVKIESEDL
                     EDGRDLEGIFSDCDGVLVPGGFGLRGIEGMVRAARYAREKRLPYFGICLGMQIMVIEY
                     ARTCLGLKDANSTEFAPESKFPVVSLLEEQVDIKAYGGTMRLGKGDTHLKPDTRIRSA
                     YGADVISERHRHRYEVSNQYRGRLEEAGLLVSAVTPDSALVESVEWPNHPWGVGVQFH
                     PEFKSKPLAPSPLFTQFIAACLKNQEQRS"
BASE COUNT          502 a          979 c          942 g          619 t
ORIGIN      
        1 ccggattggg gcggtttctg gctctgggaa gacgatgaac cgctggactg gtcctgaccc
       61 gtctggccgc tccccgccgg ctgcccgggc tgctccggct ggctgccgga tggttgggcg
      121 cctgatgatg gagcgccagc gggctgcgcg ccagccggct gctggccgtt ttgctcaggg
      181 ctggtgccct ggtccgtaga ctcctggatg gacccctgca ccccgctgtc caacgttatc
      241 tcatccgtgt ccaggttcac cgtgatccgg gccgcctgga agatgtcccc tttgcggctg
      301 acccagggca tgcccgagag ctcgaggatc ttcttgtccc ggtagtactt cgcgtattcc
      361 gcccggcaga ccaggtcctt cttgaggatc cgaaccccga tctggatgat ggtgagctgt
      421 tccttgtccc ggtcctcgat gaacccgccc ttgaccacca tctcgttctt gaggtcctgc
      481 atcatggcgt ttccctggac ccgggcgatc ttctgctggc gatcatagaa gagctgcgtg
      541 ctgctcaggt ccaggccccg cttggcatcc accaccttca cgtccccgcg gcactgggcg
      601 tagatgaaat cctttccgaa gagctcgatc tggtccgcgt cgatccgcat gtccgtagtg
      661 gccacttcgg cgtgacccgt gagcagcgca tgctgggcac cttcggccag caccgtggtc
      721 atccggtctc ccgcgtacgt gaaaacgtcc gcggcgagcg gaggagcgct gagaagcagg
      781 gccaggagcg ccgcggcggc gcggaccggg ctactccttc ttctcttgcc agacatactg
      841 accttccacg ggcccgctga acacgatctg ccggtgtcgg aagtctccca gaaagccgct
      901 gccagtgatg aacgagccat cgtctttctt caacagtacc ctctcatccg gatccgcggt
      961 gagccgcttg gtcttgtcca cccaggagag cgactcggcc cggatgttgg ctttttcccg
     1021 ggcggaatac acctggaccg caccggagat ctccgcgtcc tggctgtccg tatgaaagac
     1081 cacgtggctc gcccgtccct ccgtgtccat cgcgcccttg tcgtcgtact cggtgaaatg
     1141 ggcgtccgtg aggaccgtcg tgctcgtgga agagaaggat tccgcccttg atgcctcgag
     1201 ctggacggag acttttcccc ctttgttgac gcggtggacc agacctacgg ccaccgtgtc
     1261 cgggatgttc tccggctgct tctccacgac ggtctggtct ccgtagtcca ggctgcaacc
     1321 cgcgcagagg aggctcaaga cgagcacggc gccgacgagg gcccgtctca gcttcgctgt
     1381 tcctggttct tcagacatgc cgcaatgaac tgggtgaaga gcggactcgg ggcaaggggc
     1441 ttggacttga actcgggatg gaactgcacc ccgacgcccc aggggtggtt gggccattcc
     1501 acggattcca ccagggcgga gtccggcgtc acggcgctca ccaggagccc tgcctcctcc
     1561 agtctgccgc gatactggtt ggagacctcg tagcggtggc gatggcgctc ggagatcaca
     1621 tccgcgccgt aggcggatcg gatcctggtg tcgggcttga ggtgcgtgtc tcctttgccc
     1681 aggcgcatgg tgccgccgta cgccttgatg tccacctgtt cttccagaag actcaccacc
     1741 gggaacttgc tttccggggc gaactccgta gagttggcat ccttgagccc caggcaggtc
     1801 cgggcgtact cgatcaccat gatctgcatc cccagacaga tcccgaaata cggcaggcgc
     1861 ttctcccggg cgtaccgggc ggcccgtacc atgccctcga tcccccgcag gccgaaccca
     1921 ccgggtacca ggacgccgtc gcagtcggag aagatgccct ccaggtctct gccgtcctcc
     1981 aggtcctcac tctcgatctt gacgagctgc acgcgggagg cgttggcgat gccgccgtgg
     2041 aacagggact cgaagatcga gatgtaggag tcgtggagtc ccgtgtattt gccgacgatc
     2101 ccgacccgga cggtgcgctt ggcctccgtg aagtggtcca ccaccttctg ccagccgtcg
     2161 cggatgggcg cctcttccgc cagtccgagc ttcttgatga tcagagcatc gaccttctgc
     2221 tccacgaacg taagggggat ctcgtagatc gtggtccgca cgtcgaaggc ggagatcacc
     2281 gcctccttct ccacgttcgt gaaaagagcg atcttgcgcc ggaggtcctc gtccagtcga
     2341 ctcaccgatc ggcacaggag gatatcgggc tggatcccga tctcccgaag ctcccgcacg
     2401 gagtgctggg taggcttgct cttgggctct ccccagtaga cctgaggaac cagcgtgagg
     2461 tggacgaaga ggacgttctc cttgccaagg tcatggatga actgacgggc cgcctccagg
     2521 aagggcacgc tctcgatgtc acccaccgtg ccgccgacct ccaccagggt gacgtcgacg
     2581 cccgcctgtt ctccgacgcg gaagatccgc gccttgatct cgtccgtgat atggggaatc
     2641 acctggaccg tccgaccgag gaatcggcct tcccgctcct tcgtgatgac ggcctgatag
     2701 acttgccccg tggtgatgga gttgtcccga ccgatcggtg aggaggtgaa ccggctgtag
     2761 ttgcccaggt ccagatccgt ttccgcgccg tcgtccgtga cgtagacctc cccatgctgg
     2821 aacgggttca tggttccggc atccacgttg aggtaaggat cgatcttgat catgcggatg
     2881 ctgaagccgc ggttttcgag aaggcttcca atagaggcgg aggcaacgcc tttccccagc
     2941 ccggaacaga cgccacccgt gacgaagatg tatttgctca cggggattgg attctactat
     3001 aaaagaggga ttgtctcaac ggcgtggaga acggcacggt gg
//
LOCUS       DASRZS010000487         2954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1040623, whole genome shotgun sequence.
ACCESSION   DASRZS010000487 DASRZS010000000
VERSION     DASRZS010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040623"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VFH83_11880"
     CDS             complement(<1..722)
                     /locus_tag="VFH83_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6487114.1"
                     /translation="MSDGSEQQAILIIDDERIVLASMVQSLAEWARKRSVRLLACGSA
                     EEGLRKLEQEGNRVAVAVSDLRMPGMGGREFFLRVGKLYPEIISVLVTGFADLDEIVN
                     ALQSGVYSFILKPWDPEYLARELDKALSVQALRMENRRFLAMMHEELRLAGELQRSVL
                     GLTPPRSKSADFAVSYLPLEGIECGGDYCDVLPLGNDRWLLLIGDVAGHGVKAALVTF
                     FMKAAIGEGYLVSSRRDSAHPG"
     gene            804..1628
                     /locus_tag="VFH83_11885"
     CDS             804..1628
                     /locus_tag="VFH83_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6487115.1"
                     /translation="MDLSSSVRLNNGVEMPWLGLGVFRSPPGRETERAVRWALEMGYR
                     HIDTAALYENEADVGKAVKASGLPRDSVFITTKVWNTDQGYQNALKAFDLSRSRLGVD
                     VVDLYLVHWPIKGKFLDTWKALEKLYADGAVRAIGVSNFLVHHLEELARASSIAPAVN
                     QVEFHPFLVQPELLAYDTAHGIRHEAWAPLARAKRFDTPVLASLAQAHERTPAQIVLR
                     WEIQLGVVTIPKSVHRERIEENSRIFDFELSEEDMRKVSSLDSGERIGPHPDSVTS"
     gene            1640..2410
                     /locus_tag="VFH83_11890"
     CDS             1640..2410
                     /locus_tag="VFH83_11890"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET6487116.1"
                     /translation="MPARPLAPATPAIPRRRLLVLGHRGYRARYPENTLLAFRSALEA
                     GADGVECDLQKTADGHYVVIHDPTVDRVTGAAGAVARMSLAELRDLDFGRGERIPELS
                     DLLSILPADSYLDLELKEETLTSADCEPILRILADRTDPERLMISSFEPELLRPFRAR
                     GCVVGFLVGDRMMESGPQGLLSVLGGLRPQYVNLPVETFSVLGRRRARFLVGSLRMMG
                     CRVLWWTVNEPAELALVARGARIIVTDCVEEILRAAGR"
     gene            complement(2445..>2954)
                     /locus_tag="VFH83_11895"
     CDS             complement(2445..>2954)
                     /locus_tag="VFH83_11895"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET6487117.1"
                     /translation="LLGPGMVTGLRITTENPAEVGADLVANAVAAHERVHGSCVVVDF
                     GTALSFTAVSSAGELRGVAIAPGLSASVDALARTTAQLPRIPLSPPPRAIGRNTVHSI
                     QSGVVLGCVALVEGLIGRIRAELEPDARVIATGGQSPLIAPLLTEPAIVEPWLTLEGL
                     RIVSQRTVA"
BASE COUNT          485 a         1006 c         1000 g          463 t
ORIGIN      
        1 tccccggggt gagccgaatc ccggcgggag gagaccaggt agccttcccc gatggccgcc
       61 ttcatgaaga acgtgacgag ggcggccttg acgccgtggc cggccacgtc cccgatgagc
      121 aggagccacc ggtcgttccc caggggaagc acgtcgcagt agtctccgcc gcactcgatg
      181 ccctccaggg gaaggtagga gaccgcaaag tccgcgctct tggagcgcgg aggggtgaga
      241 cccagcacgg aacgctgcag ctctccggcg agtcgaagct cctcgtgcat catggcgagg
      301 aagcggcggt tctccatccg cagcgcctgc acggacagcg ccttgtccag ctctcgggcg
      361 aggtactccg ggtcccaggg cttgaggatg aacgagtaga cgccgctttg cagcgcgttc
      421 acgatctcat ccaggtcggc gaagccggtc accaggacgc tgatgatctc cgggtagagc
      481 ttgccgacgc ggaggaagaa ctcgcgtccg cccatccccg gcatgcggag gtccgacacg
      541 gcaacggcca cgcggtttcc ctcctgctcg agcttgcgca gaccttcctc cgcggacccg
      601 cacgccagga gccgaaccga cctcttgcgg gcccactcgg ccaggctctg caccatggag
      661 gccaggacga tcctctcgtc atcgatgatc aggatcgcct gctgctcgga accatcgctc
      721 atgtaaggcg agaataccat cagggtcgga aaatggtata gtatgcggga ccggaagttc
      781 ccgttcgtgg tcgagaggag cctatggacc tttcatcgtc ggtgcgtttg aacaacggcg
      841 tcgagatgcc atggctcggg ctgggcgtat tccgctcccc tccgggacgg gagacggagc
      901 gcgcggtccg ctgggccctg gagatgggct accgtcatat cgacaccgcc gccctctacg
      961 agaacgaggc ggacgtcggc aaggcggtca aggccagcgg actgccgcgg gattccgtgt
     1021 tcatcacgac caaggtctgg aacacggacc agggctacca gaacgcgctc aaggcgttcg
     1081 atctcagccg atccaggctc ggggtcgacg tggtggacct gtacctcgtg cactggccca
     1141 tcaagggcaa gttcctggac acctggaagg cgctggagaa gctctacgcg gacggcgcgg
     1201 ttcgggccat cggggtgagc aacttcctgg tgcatcacct ggaggagctt gccagggcct
     1261 cctccatcgc gccggcggtg aaccaggtgg agttccatcc cttcctggtt cagccggagc
     1321 tcctggctta cgacaccgcc cacgggatcc gccacgaggc atgggcgccg ctggcccgcg
     1381 ccaaacggtt cgacacgccg gtcctcgcgt ccctggcgca ggcgcacgag cggacgcccg
     1441 cccagatcgt gctgcgatgg gagatccagt tgggggtggt caccatcccc aagtccgtgc
     1501 accgcgagcg catcgaggag aacagccgca tcttcgattt cgagctgtcg gaagaggaca
     1561 tgcggaaggt gtccagcctg gactccgggg agcgcatcgg accccatccc gactcggtga
     1621 cctcctgagc gccgcggaca tgcctgcgcg cccgcttgct cccgccacgc ccgccatccc
     1681 acgacgccgg ctcctggtcc tgggtcaccg ggggtaccgc gcccgttacc ccgagaatac
     1741 actcctggcg tttcgaagcg cgctggaagc cggcgcggac ggcgtggagt gcgacctgca
     1801 gaagaccgcg gacggccact acgtcgtgat ccacgatccc accgtggacc gggtcaccgg
     1861 ggccgccgga gcggtggcgc gcatgagcct ggccgagctt cgcgatcttg acttcgggcg
     1921 gggagaacgg atccccgagc tttccgacct tctgtccatc cttcccgcgg attcctacct
     1981 ggacctcgag ctcaaggagg agacgctcac gtccgccgac tgcgagccga tccttcggat
     2041 cctcgcggac cgaacggatc ccgagcgcct catgatctcg tccttcgagc ccgagctgct
     2101 ccgcccgttt cgggcgcggg gctgcgtggt gggattcctg gtgggcgacc ggatgatgga
     2161 gagcgggcct cagggcttgc tctccgtgtt gggcggcctg cggccccaat acgtcaacct
     2221 tccggtggaa accttctcgg tcctgggcag acgccgggca cgattcctgg ttgggtcgct
     2281 gaggatgatg ggctgccgcg tcctctggtg gaccgtcaac gagcccgccg agctggccct
     2341 cgtcgctcgc ggcgcgcgga tcatcgtgac cgactgcgtg gaggagatcc tgcgggccgc
     2401 cggccgctag gggctgcaag ccggccgccc gggcgccacc acccctaagc cacggtcctt
     2461 tgcgaaacga tcctcaggcc ctcgagggtc agccatggct ccacgatcgc cggttccgtg
     2521 aggagcgggg caatgagcgg ggactgaccg ccggtggcga tcacccgcgc gtccggctcg
     2581 agctctgccc ggatcctccc gatcagccct tccaccaggg cgacgcaccc cagcacgacg
     2641 cccgattgga tggagtggac cgtgttcctg ccgatggcgc gcggaggggg ggagagcggg
     2701 atccggggaa gctgagccgt ggttcgcgcc agggcatcca cggacgccga aagcccgggc
     2761 gcgatggcca ccccgcgaag ctccccggcg gaggagaccg cggtgaacga gagcgcggtg
     2821 ccgaagtcca ccaccacgca actcccgtgg acgcgttcgt gggccgccac ggcgttggca
     2881 accaggtccg cgccgacctc cgccgggttc tccgtggtga tccgaagccc ggtcaccatc
     2941 cccgggccca ggag
//
LOCUS       DASRZS010000488         4524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_649222, whole genome shotgun sequence.
ACCESSION   DASRZS010000488 DASRZS010000000
VERSION     DASRZS010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649222"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /gene="aroC"
                     /locus_tag="VFH83_11900"
     CDS             complement(<1..945)
                     /gene="aroC"
                     /locus_tag="VFH83_11900"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET6487118.1"
                     /translation="MPGSSFGRVLCITTFGESHGPGIGVVLDGVPPGLALTAADIQHD
                     LDRRRPGQSAITTPRGEKDRVEIVSGVFQGITMGTPIALLIRNEDIKSSDYNNLKDLV
                     RPGHADWTYRAKYGVRDWRGSGRASGRETAARVAAGAVARKVLAARGASVLGYAVEIA
                     GIRARSMDPAEIERNMVRCPDAAAAALMVQRIEEARAADDSVGGVIEVTAHGCPAGLG
                     DPVFDKLEALLAHAIMSVGAIRGVEIGAGFSATGLRGSAFNDEPYMEDGRFRTRTNNA
                     GGIAGGISNGEDIVLRAAVRPPASIARPQSTVDTSGGAT"
     gene            complement(959..1513)
                     /locus_tag="VFH83_11905"
     CDS             complement(959..1513)
                     /locus_tag="VFH83_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487119.1"
                     /translation="MRNLYTFLEWLGLILGGILAGSAIAVLRVRRRIVKQVETELGKG
                     TLLLAAGARVEIPVPQSGNGGSAARLREEGSSGMRSTAVRSAARASSTRTTTVSASGV
                     VMLLSKGLYFHSWIGHHQVFIAGASITWIGVTEAPSGHRSARPCIAVRYLRADGKEDG
                     ITIRLVSPAQWVNAVKTHLITRSE"
     gene            complement(1635..2438)
                     /locus_tag="VFH83_11910"
     CDS             complement(1635..2438)
                     /locus_tag="VFH83_11910"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HET6487120.1"
                     /translation="MRKHVVFFFTGTGNSLKVSKDIASALGDCELVPMGACTQYDLTA
                     RYETIGFVFPTYYRGIPRKVREFVERLNLLRDDHAYYYAVTTMGKHDGNALHQIMTLL
                     GRKGIALNYAQALDMYSNYVISYDMRDTVVEEAEQSEVCFQPILRAILDRKTNHVPRA
                     RLLDELAYRIWARRAPRMDRFYSVSDNCSHCGVCEGVCPVGNIILDGQGRPHFQHHCE
                     QCVACIQFCPPRAINYRDKTQNRGRYRNPEVSAARLSELYRRARTGPRP"
     gene            complement(2459..4132)
                     /locus_tag="VFH83_11915"
     CDS             complement(2459..4132)
                     /locus_tag="VFH83_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6487121.1"
                     /translation="MADGTPAATADGREPTAAVDARPASAGSGRPAPAAPQESIRILV
                     IDDEEGMREGMRRVLERRGFSVRTARDGETALRLLEEEACDVALVDLKMPGIDGFKVT
                     EHISRRFSGRTVVVIVSALATVEAAVEVTQHGAFDFLVKPFTPDDLLQVLARAIQQWR
                     LIRERERYLSELASERNLSHQMISSMHEGVVVLNIRREPVLMNPRAEHFLGKRFREGI
                     TMADLFPDLDAQSAIDGVIEAAGTRPECRIVQLAVGAAAGFGGSVSRITGGYEGMLQL
                     SVTPLLREEDQRGDPYGVIVILSDITASWKAEQDKNRFVSMVAHELKSPLSAIINYIN
                     VIQTGMFDGNLPKIHEMLERCKVRGEALLDLVRDLLYINSREVGRVQRSIEDLDLKAV
                     LSAQLEFFRVQADRRRIVVSLQAPDQPCPVKADRGDLDRIFMNLISNGIKYNRERGSL
                     NVRLSEADGRWEVAVSDTGIGMSPAETANLFQEFYRVKSHKTSGIAGTGLGLATVKRV
                     LSEYGGRISIESKPDAGSTFTITLPRGDAAAKGDTARGDAAKGGEAPKA"
     gene            complement(4125..>4524)
                     /locus_tag="VFH83_11920"
     CDS             complement(4125..>4524)
                     /locus_tag="VFH83_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6487122.1"
                     /translation="RILIVDDDVDLLAGQKEFLQAKGYEVLTAGTIEDGLKTAGESTP
                     DLVIADLMMEHYDSGFVFCKKLRDLPGLSKVPILMQTAAPRKVGFTFEAGAAPGREWM
                     KADEVLTKPVPLEDLVGKIEQHLAGSARHG"
BASE COUNT          750 a         1537 c         1449 g          788 t
ORIGIN      
        1 ggtggcgccg ccggaggtgt ccaccgtgga ctgggggcgc gcgatggatg cgggaggccg
       61 cacggccgcc cgcaggacga tgtcctcacc gttggaaatg ccaccggcga tgccacccgc
      121 gttgttggtg cgggtgcgga agcggccatc ctccatgtag ggctcgtcgt tgaacgcgga
      181 tccccgcaag cccgtggccg aaaaccccgc gccgatctcc acgccgcgga tggcgccgac
      241 cgacatgatg gcgtgcgcca ggagcgcctc cagcttgtcg aagaccggat cacccagccc
      301 ggccgggcac ccatgggccg tcacctcgat gacccctccc acggaatcat ccgccgcgcg
      361 cgcctcctcg atcctctgaa ccatgagggc cgcggccgcg gcgtccgggc agcggaccat
      421 gttccgttcg atctccgcgg gatccatgga gcgcgcccga atgccggcga tctccaccgc
      481 gtaccccagc accgaagccc cccgcgcggc gaggaccttg cgcgcgacgg ctcccgcggc
      541 tacgcgcgct gcggtctccc ggccggatgc ccggccgctc cctcgccagt cccgcacacc
      601 gtacttggcg cggtaggtcc agtccgcgtg accgggccgg accaggtcct tcaggttgtt
      661 gtagtcggag ctcttgatgt cctcgttgcg gatgagaagg gcgatcgggg tccccatggt
      721 gattccctgg aagacaccgg agacgatctc cacgcgatcc ttctcccctc ggggggtggt
      781 gatagcactc tgccccgggc gacgccggtc caggtcatgc tggatgtcgg cagcggtcag
      841 cgcgaggccc ggcggaactc cgtccagcac cactccgatc ccggggccgt ggctctctcc
      901 aaaggtcgtg atgcacagca ctctcccaaa ggaactcccc ggcacgctgg atcctccttc
      961 actcggagcg ggtaatgaga tgggtcttta ctgcgttgac ccactgggcc gggctgacga
     1021 ggcggatcgt gattccatcc tccttgccgt cggcccgcag ataacgtacc gcgatacacg
     1081 gacgggcgga ccggtgaccg gacggggcct ccgtcacgcc gatccaggtg atcgatgccc
     1141 cggcgatgaa tacctggtgg tggccgatcc aggaatggaa gtagagcccc ttggagagca
     1201 gcatcacgac accagaggcg ctgacggtgg tggtccgggt gctggaggcg cgcgcggcgg
     1261 agcgcacggc ggtggaccgc atgccggagg atccttcctc acgaagccgg gcggcggatc
     1321 cgccgttgcc cgactgggga acggggatct ccacacgggc gcccgcggca aggagcagcg
     1381 tcccctttcc gagctcggtc tccacctgtt tcacgatacg gcggcgtaca cgcagaaccg
     1441 cgatagcgga accggcaagg attccgccca ggatcagacc aagccattcg aggaaggtat
     1501 agagattccg catgggcccg gggagaaagt acagaaaacc gccgtgggct gcaaggagtc
     1561 ggagacctgc cgaagagctt ccgcggaccc cgccccaaaa gcggcggtat ggcgaagggc
     1621 ctgggccgcg ccgcctatgg gcgggggccc gtccgtgccc tacggtacag ctcggacagc
     1681 ctggcggcgc tcacttccgg attcctgtat ctccctctgt tctgtgtctt gtccctgtag
     1741 ttgattgctc tcggcgggca gaactggatg caggcgacac actgctcgca gtgatgctga
     1801 aaatgaggcc gcccctgacc atccaggatg atattgccca ccgggcagac accttcacag
     1861 accccacagt ggctgcagtt gtccgagacg ctatagaacc tatccatccg cggcgcgcgc
     1921 cgagcccaga tccggtaggc cagctcgtcc agcagcctgg cgcgaggaac atgattggtc
     1981 tttcggtcca gaatagcccg caaaatcggc tggaagcaga cctccgactg ttctgcttcc
     2041 tcgaccacgg tatcgcgcat atcgtaggag atgacgtagt tcgagtacat gtcgagagcc
     2101 tgggcatagt tgagagctat gccctttctc ccaaggaggg tcatgatctg gtgcagtgcg
     2161 ttaccgtcat gcttgcccat cgttgtgacg gcgtagtagt aggcatgatc gtcgcgcagg
     2221 agattcagtc tctcgacaaa ctccctgact tttctgggaa tcccgcgata gtacgtaggg
     2281 aagacgaaac cgattgtttc gtatctcgcg gtgaggtcgt actgggtaca ggcgcccatg
     2341 gggacaagct cgcaatcgcc cagagccgat gcgatgtcct ttgacacttt caggctgttt
     2401 cccgtccccg taaagaagaa tacaacgtgc tttctcattc agcgcggaag cggttcgctc
     2461 aggccttcgg agcttccccg cccttggcgg cgtcccctcg ggcagtgtcg cccttggcgg
     2521 cggcgtcccc ccggggcagc gtgatggtaa acgtggagcc cgcatccggc ttggactcga
     2581 tcgagatcct tccgccgtat tcgctcaaga cgcgcttcac ggtagccagc cccaaccccg
     2641 tgcccgcaat gcccgaggtc ttgtggctct tcacgcggta gaactcctgg aacaggttgg
     2701 cggtctccgc cgggctcatc ccgatgcccg tgtcgctcac cgccacttcc cagcgaccgt
     2761 ccgcctcgga gaggcgcacg ttcaggctgc cgcgctcgcg gttgtacttg atcccgttgg
     2821 agatcaggtt catgaagatg cggtccaggt ctccccggtc cgccttgacc gggcagggct
     2881 ggtccggcgc ctggaggctc accacgatgc gccgccgatc cgcctgcacg cggaagaact
     2941 cgagctgagc cgacagcacc gccttcaggt ccaggtcttc gatggagcgc tgcacccgcc
     3001 ccacctcgcg gctgttgatg tacaggaggt cccgcacaag gtccaggagc gcctctccgc
     3061 gcaccttgca tcgctccagc atctcgtgga tcttgggcag gttcccatcg aacatgccgg
     3121 tctggatcac gttgatgtag ttgatgatcg cggagagcgg gctcttgagc tcgtgggcaa
     3181 ccatcgacac gaaacggttc ttgtcctgct ccgccttcca gctcgccgtg atgtcggaga
     3241 ggatcacgat caccccgtac ggatcccctc gctggtcctc ttccctcagg agcggagtga
     3301 ccgaaagctg gagcatcccc tcgtagccgc ccgtgatccg ggataccgat ccgccgaagc
     3361 ccgcggcggc gccgacggca agctgaacga tccggcattc cggccgggtg cccgcggcct
     3421 cgatcacgcc gtcgatggcg gactgggcat cgaggtccgg aaacaggtcc gccatggtga
     3481 tcccttcccg aaaccgcttg cccaggaagt gctcggcgcg ggggttcatg agcaccggct
     3541 cgcggcggat gttgaggacc accacgccct cgtgcatgga agagatcatc tggtgggaga
     3601 ggttgcgctc gctcgcaagc tcggagaggt agcgctcccg ctcgcggatg agccgccact
     3661 gttggatggc ccgtgcgagg acctggagca ggtcgtccgg agtgaagggt ttcaccagga
     3721 agtcgaaggc gccgtgctgc gtgacctcca ccgcggcctc cacggtggcc agggcggaga
     3781 cgatgaccac gacggtccgc ccggagaatc gccggctgat atgctcggtg accttgaacc
     3841 cgtcgatgcc gggcatcttc aggtccacca gggccacgtc acacgcctcc tcctccagga
     3901 ggcgcagcgc cgtctctccg tcccgagcgg tgcggaccga gaagccgcgc cgctccagca
     3961 cccgccgcat gccctcacgc atgccctcct cgtcgtcgat caccaggatg cggatcgact
     4021 cctgcggggc ggcgggggcg ggccgaccgc tcccggcgga tgccggccgc gcgtcgaccg
     4081 cggcggttgg ctcccgcccg tcggccgtgg cggcgggagt cccgtcagcc atggcgagcc
     4141 gagccagcca gatgctgctc gatcttgccc accaggtcct ccaggggaac cggcttggtg
     4201 agcacctcgt ccgccttcat ccattccctt cccggcgccg cccccgcctc gaaggtgaac
     4261 cccaccttcc gcggcgcggc ggtctgcatc aggatgggca ccttggagag acccggcagg
     4321 tcccgcagct tcttgcagaa cacgaacccc gagtcgtagt gctccatcat caggtccgcg
     4381 atcaccaggt ccggagtgga ctctccggcg gtcttcaggc cgtcctcgat ggtgcccgcg
     4441 gtcaggacct cgtagccctt ggcctggagg aactccttct gtcccgccag caggtccacg
     4501 tcgtcatcca cgatcagtat gcgc
//
LOCUS       DASRZS010000489         7453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1425326, whole genome shotgun sequence.
ACCESSION   DASRZS010000489 DASRZS010000000
VERSION     DASRZS010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425326"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFH83_11925"
     CDS             complement(<1..213)
                     /locus_tag="VFH83_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487123.1"
                     /translation="MGSRVRSIVTARLLCYYRGAMVLFLLFSLPALYALVVLLPWDPW
                     KRPSTFVLVSSFFKGVLLFFPGYLLIL"
     gene            308..1360
                     /locus_tag="VFH83_11930"
     CDS             308..1360
                     /locus_tag="VFH83_11930"
                     /inference="COORDINATES: protein motif:HMM:NF028488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET6487124.1"
                     /translation="MTILVSNDAMHQAHLAVEEARMHQALSRDRVREAVTILKSSYQV
                     VAPTAKGKSFTFSQVDDAASVTLEYSSTILPLKKFFLPPVEVLLTYDTVRQTARAPEP
                     VAEPLLFFGVHNYDLQGLLRLDHAFENGVPDPTWLARRSKAVFVGVSYTPDEHHFSPS
                     VGIPTDRKDGFDLFLTRFGDHYRVESLTKTGDELVSLLRPILSTCDCPGEPVVQFKNK
                     LRQSVAGIREVFHGAYNHPVWQETARRCLSCGTCNISCPTCYCFDVDDQTRLGGTEGD
                     RLRRWDSCQFRSFSTVAGGEAFRDRRRDRVRHRMSRKFAYITDGAGQPFCVGCGRCVR
                     QCAAGINIVEVINALL"
     gene            1368..2204
                     /locus_tag="VFH83_11935"
     CDS             1368..2204
                     /locus_tag="VFH83_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HET6487125.1"
                     /translation="MMEMRNIYLPEQAELVRVEKLTDTERLFEVMLPGRRDLGHQPGQ
                     FVQVSLYGIGEAPISICSSPLAHGTFEMVVRNVGNVTAALHRLSAGDSVGIRGPFGKG
                     FPLEELWGKDVLFVAGGIGLPPLRSLINTLLDGAYRTKIRRLIILYGTRSPAEFLFGE
                     ERTGWESRKDVEYQVTVDRPASGWNGHVGVITTLLPELTLDFSNTVAVVCGPPVMFKF
                     AILALHSKGLRDEQILLSLERKMKCGVGKCGHCQINNVYVCQDGPVFSYARIRDLKEA
                     IQ"
     gene            2201..2956
                     /locus_tag="VFH83_11940"
     CDS             2201..2956
                     /locus_tag="VFH83_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HET6487126.1"
                     /translation="MSKPKVAFFDFTSCEGCQLSKLNLEDDLLDILGMVELVNFREAI
                     SDRREDYEIAFVEGSISTPHCVERIHHIRRQAKILVALGACAHIGGVNAIRNRMDIDA
                     VKREVYGEDRYLFPSIKAQAVGDVVKVDCVVPGCPIDNQELKRTLSALFTGKLPSLPE
                     YAVCVECKARENECLYDYGTVCMGPITRAGCSAACPSNGHYCFGCRGVLPDLNKNAAH
                     DVMEKHKLSEHQIRNRFTMFNTNLVQLEEKRDE"
     gene            2949..4265
                     /locus_tag="VFH83_11945"
     CDS             2949..4265
                     /locus_tag="VFH83_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HET6487127.1"
                     /translation="MNERTMSIRVPYVTRVEGHGNIVIDMREGKLVTSHLEVVEPPRY
                     FEAMLKGYPYSDVALITSRICGICSVGHQMTSLKATERALGITVTDQTVLLRKLLVYG
                     ATMQSHVLHIFFLAAPDLLGAPSVVPLAASHPDVVKMALRMKKLSNDICDRLGGRTIH
                     PNRLVPGGFTKLPSMEDLRWIQRMLETQMVPDAKASVDLLASLASKFPAFHRETEYVG
                     LRSDQEYAFYDGQIASTDTGLSPVSDYRSITNEFVVPHSTSKHCRHARGSYSVGALGR
                     LNNNFDKLNPMARGVAERLGLKPVVTNPFLNTAAQLVETVHVVEESLKILDQLLTRGI
                     QDEKPVGPGQLREGRAAEATEVPRGILFHDYTYDNRGQIVKANCIIPTTQNTQCIDDD
                     MSELVPWMISQDMSEEKMTLTLEMLVRAYDPCISCSVHFLDVEFRR"
     gene            4285..4737
                     /locus_tag="VFH83_11950"
     CDS             4285..4737
                     /locus_tag="VFH83_11950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HET6487128.1"
                     /translation="MTIVAVGNETMGDDGIGPFLAGLVRERREAGSWPTQTEVLCADP
                     TMAVCCAAEGTPVLVIDAADMGLEAGEWRAVSAREVLPYAGGKGSTHSMSVSQAIQMA
                     DALGCLDRLRILAVQWGSLEPGSQLSAPVRERVPAMLDWIEKEAQRWP"
     gene            4734..5117
                     /locus_tag="VFH83_11955"
     CDS             4734..5117
                     /locus_tag="VFH83_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6487129.1"
                     /translation="MKRILVVDDDRDVFESMKIVLEAEGYDVDWATNGTEGITKARAC
                     RPDLMILDVMMNTDDEGFQVTYQMKRDPKLSCIPIVMVTSVGARTGFSFDRNRDQDYL
                     PVNEFLEKPVEPKVLLDKVRENLGH"
     gene            complement(5154..6218)
                     /locus_tag="VFH83_11960"
     CDS             complement(5154..6218)
                     /locus_tag="VFH83_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET6487130.1"
                     /translation="MEKVILKAHDLDGYLKEADRATLDEVDARFSAALERFATLADPG
                     RSAQHDRVMAELFDLYNDLGRVMREIVDREPRIHVYPFQTPREVHGEVSRLIAKLRDE
                     RTAQPEFVYYTQRAYELLFNFAFGSASHEKKNHIVVRTPVRMPVQNYAVHKIPDVDER
                     IESSVMCVMLRGALLPGMIMSKEIQEYSSHGYVTPFALFKISRDDRKDERTMEYVLSL
                     DRSYFDLSQLQGKDLFFADPMNATGGSLVTVVNYLSSKGVAPRSIKFFNIIAGLKGAL
                     RIVRALERAEIYTLWMDPVLNSKAYILPGLGDAGDRINGRDDGEKPRNIIQLISDYGS
                     QIVSLYREQVRTIEQTVLGG"
     gene            6300..>7453
                     /locus_tag="VFH83_11965"
     CDS             6300..>7453
                     /locus_tag="VFH83_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HET6487131.1"
                     /translation="MLRKRYRFRGTVQGVGFRPAISRIASGLGLDGFVQNRSSEVVAE
                     VRGTEEAISRFEAALQTGLPPAARLERIQAEVVESPAETSEALAESDGFLILESEPDL
                     YSFPPIPPDLPLCPECAAELLDPGNRRYLYPFITCTQCGPRYSIVERTPFDRRNTSMR
                     PFEPCPACAREYRDPVDRRFHSQTNSCPTCGPRLTLLDGAGRRLTTDPIPAAMDLLAD
                     GGIVAVEGIGGFHLAAVPAQEGAVQRLRRDKERERKPFALMVQDLAEATKICRLSPAE
                     VEALSSSQAPIVIAARAPDAPDWLRAVSDTDTLGIMLPYTPLHLLLFRHPDRPPRYRC
                     LVMTSGNRAREPIITDPADAVGKLLGVADAFLVHDRRIVLRTDDSIVRVG"
BASE COUNT         1327 a         2404 c         2413 g         1309 t
ORIGIN      
        1 caggatcagc aggtagcctg ggaaaaacag gagcacgccc ttgaagaatg aggacaccag
       61 gacgaacgtc gagggccgct tccagggatc ccacggcaag agcacgacca acgcatacag
      121 ggccggcagc gaaaagagca ggaacagcac catcgcgccg cgatagtagc agagcagtct
      181 ggcagtgaca atagatcgga ctcgggaccc cacccgggcg aggtctctct ggatcgtccg
      241 gtcctgggcg atcgattcga catcgagatc gctattctag atcaatcgcg gtacaattct
      301 ctatatcttg acaattctcg tcagcaatga tgccatgcat caggcccacc ttgccgtgga
      361 ggaggcgcgt atgcaccagg cgctttcgag ggaccgagtc cgcgaggccg tgaccatcct
      421 taagtccagc taccaggtgg tggcaccgac cgcgaagggc aagagcttca ccttctctca
      481 ggtagacgat gcagcatcgg tcaccctcga gtattccagc accatcctgc cattgaagaa
      541 gttcttcctg ccgcccgtag aggtgctcct gacctatgac acggtccggc agacggcccg
      601 cgcgccagag cccgttgcag agccgctcct cttcttcggc gtgcacaact acgaccttca
      661 gggcctgttg aggctcgatc acgcgtttga gaacggggta ccggacccga cctggctggc
      721 cagacgctcc aaagccgtgt ttgtcggcgt cagctatacg ccggacgagc atcatttctc
      781 cccgtccgtc ggcattccga ccgatcggaa ggacggcttc gatctgttcc tcacgcgatt
      841 cggggatcac taccgggtgg agagcctgac gaaaaccggg gacgagcttg taagcctgct
      901 gcggccgatc ctgtccacct gcgactgccc cggtgagccg gtcgtccagt tcaagaacaa
      961 gctgcggcag tcggttgccg ggatccggga ggtcttccac ggggcctaca accatccggt
     1021 gtggcaggag acggcccgac gctgtttatc ctgcggcacc tgcaacatct cctgccccac
     1081 ctgctactgc ttcgacgtgg atgaccagac ccgtctcggt gggacggagg gagaccggct
     1141 ccgccgctgg gactcctgcc agttccgttc cttctcgacg gtggcgggcg gtgaggcctt
     1201 ccgcgatcga cgcagggacc gggtccgcca tcggatgtcg cgcaagttcg cctacatcac
     1261 ggacggggcc ggccagcctt tctgcgtagg ctgcggccgg tgcgtccgcc agtgcgccgc
     1321 tggaatcaac atcgtcgaag tgatcaacgc tctcctgtag gaggtggatg atggagatga
     1381 ggaacatcta cctgcccgag caggcggagt tggtgcgggt ggagaagctg accgacaccg
     1441 agcggctctt cgaggtgatg ctgccgggcc gccgggacct gggacatcag cccggccagt
     1501 tcgtgcaggt ctccctctac gggatcgggg aggcgcccat ctcgatctgc tcctcgccgc
     1561 tggcgcatgg caccttcgag atggtggtgc gcaacgtcgg caacgtgacc gccgcgctcc
     1621 atcgcctttc ggcgggggat tccgtgggga tccgcggccc ctttgggaag ggcttcccgc
     1681 tggaagagct ctggggaaag gatgtcctgt tcgtggcggg cggcatcggg ctcccgcctc
     1741 tgcgctcgct catcaacacg ctgttggatg gggcctaccg cacgaagatc cggcggttga
     1801 tcatcctcta cgggacccgg agtcccgcgg agttcctctt cggggaagag cgaacggggt
     1861 gggagtcccg caaggacgtg gagtatcagg tgaccgtgga tcggccggcg agcggctgga
     1921 acgggcacgt gggcgtcatc acgacgctcc tcccggagct gacgctggac ttctccaaca
     1981 cggtggccgt ggtctgcggg cccccggtga tgttcaagtt cgccatcctg gcccttcatt
     2041 ccaaggggct tcgggacgag cagatcctcc tgtccctgga gcgcaagatg aagtgcggcg
     2101 tgggcaaatg cggccactgc cagatcaaca acgtctacgt ctgccaggac gggccggtgt
     2161 tcagctacgc gcgcatccgt gacctgaagg aggcgatcca gtgagcaagc cgaaagtcgc
     2221 cttcttcgac ttcacctcct gcgagggctg ccagctctcc aagctgaacc tggaggacga
     2281 cctgctggac atcctcggga tggtggagct cgtgaacttc cgcgaagcca tctccgatcg
     2341 ccgcgaagac tacgagatcg ccttcgtgga aggctccatc agcacccctc actgcgtgga
     2401 gcggatccac cacatccgcc gtcaggcgaa gatcctggtg gcgttgggcg catgcgcgca
     2461 catcggcgga gtcaacgcca tccgaaaccg catggacatc gacgccgtga agcgggaggt
     2521 ctacggggag gaccgctacc tcttcccatc gatcaaggcc caggccgtcg gggacgtggt
     2581 caaggtggac tgcgtggttc ccggctgccc catcgacaac caggagctga agcgcaccct
     2641 gtccgcgctc ttcaccggca agctgccgtc ccttcccgag tacgccgtgt gcgtggagtg
     2701 caaggcgcgg gagaacgagt gcctgtacga ctacgggacc gtctgcatgg gtccgatcac
     2761 gcgcgccggc tgttccgccg cctgtccctc caacggacac tactgcttcg gctgtcgggg
     2821 agtgctgccg gacctgaaca agaacgccgc gcacgacgtc atggagaagc acaagctctc
     2881 cgagcaccag atccgaaacc gtttcacgat gttcaacacg aacctggtcc agcttgagga
     2941 gaagcgcgat gaatgagcgc accatgagca tccgcgtgcc gtacgtcacg cgggtggaag
     3001 gccacgggaa catcgtcatc gacatgcggg aagggaagct cgtgacctcc cacctggaag
     3061 tcgtcgagcc tccgcgctac ttcgaggcca tgctcaaggg gtacccctat tcggacgtgg
     3121 cgctgatcac ttcccgcatc tgcggcatct gctcggtggg ccaccagatg acgtccctga
     3181 aggctacgga acgggcgttg gggatcaccg tgaccgatca gacggttctg ctccgcaagc
     3241 tgctggtcta cggggcgacc atgcagagcc acgtcctgca catcttcttc cttgccgccc
     3301 cggacctcct gggcgctccc tcggtggtcc cgctggcggc gagccatccc gacgtcgtga
     3361 agatggcgct tcggatgaag aagctctcca acgatatctg cgatcggctt ggaggccgca
     3421 ccatccatcc caaccgcctt gtgccgggcg gcttcacgaa gctgccttcc atggaagacc
     3481 tgcggtggat ccagcggatg ttggagaccc agatggtgcc ggacgccaag gcgtccgtgg
     3541 acttgctggc cagcctcgcg tcgaagttcc cggcgttcca ccgggagacc gagtacgtgg
     3601 gtctgcggtc ggaccaggag tatgcgttct acgacggcca gatcgcctcc acggataccg
     3661 gactctctcc cgtgagcgac tatcgctcga tcaccaacga gttcgtggtg ccccactcca
     3721 cgtcgaagca ctgccgccat gcgcgcggct cgtactccgt cggcgccctg ggtcggctga
     3781 acaacaactt cgacaagctg aaccccatgg cgcgcggcgt ggcggaacgg ttgggcctga
     3841 aacccgtcgt gaccaatccc ttcctcaata cggcggcgca gctggtggag acggttcacg
     3901 tcgtggagga atccctgaag atcctggacc agcttctgac ccggggcatc caggacgaga
     3961 agccggtggg gccggggcag ctacgggaag gacgagccgc ggaggccacg gaagtccctc
     4021 ggggcatcct cttccacgac tacacctacg acaaccgcgg ccagatcgtg aaggccaact
     4081 gcatcatccc cacgacccag aacacgcagt gcatcgacga cgacatgagc gagctggtgc
     4141 cctggatgat ctcccaggac atgagtgagg agaagatgac cctcaccctg gaaatgctgg
     4201 tgcgggcgta tgacccgtgc atctcctgct cggtccattt cctggacgtc gagttccgcc
     4261 ggtgaacgat cgggggcact gtcggtgacc attgtggcgg tgggcaacga gaccatgggc
     4321 gatgacggga tcgggccctt tctggccggc ttggtgcggg agcggcggga ggccggctcc
     4381 tggcccacgc agaccgaggt gctctgcgca gaccccacca tggcggtctg ttgcgccgcg
     4441 gaaggcacgc cggttctggt gatcgatgcg gcggatatgg gtctggaagc gggagaatgg
     4501 cgcgccgttt ccgctcggga agtcctgccg tacgcgggcg gaaagggctc cacgcactcg
     4561 atgtccgtat cccaggcaat ccagatggcg gacgccctgg ggtgtctgga tcgccttcgt
     4621 atcctggccg tgcagtgggg cagcctggag cccggctccc agctttccgc cccggtgcgg
     4681 gagcgcgtgc cggcgatgct ggattggatc gaaaaggagg cgcagcggtg gccatgaagc
     4741 ggattctcgt ggtcgatgac gaccgggacg tgttcgaatc catgaagatc gttctggagg
     4801 ccgagggcta cgacgtcgat tgggcgacca acggcaccga gggcatcacc aaggcgcgcg
     4861 cctgccggcc cgatctcatg atcctcgatg tcatgatgaa cacggatgac gagggcttcc
     4921 aagtcaccta ccagatgaaa cgggacccga agctctcctg catcccgatc gtgatggtca
     4981 cgagcgtcgg ggcgcggacc ggtttctcct ttgaccgaaa ccgggaccag gactacctgc
     5041 cggtcaacga gttcctcgag aagccggtgg aaccgaaggt cctcctggac aaggtccggg
     5101 agaacctggg gcactagcgg gctcggccag gctcgacatc gcggtcacac cctctatcct
     5161 cccagcaccg tctgctctat ggtgcggacc tgctcccgat acagcgagac gatctggctg
     5221 ccgtagtccg agatgagctg gatgatgttg cgaggcttct ccccatcgtc cctgccgttg
     5281 atccggtctc ccgcgtcccc aaggccgggc aggatgtacg ccttggagtt gagcacgggg
     5341 tccatccaga gcgtgtagat ctccgcgcgc tccagcgccc gcacgatgcg cagggcgccc
     5401 ttgaggccgg cgatgatgtt gaagaacttg atggagcggg gcgccacgcc cttgctcgac
     5461 aggtagttca cgaccgtaac caggcttccg cccgtggcat tcatggggtc cgcgaagaag
     5521 aggtccttcc cctggagctg ggacaggtcg aagtacgaac ggtccaggct gagcacgtat
     5581 tccatcgtgc gctcgtcctt gcggtcgtcc cgtgagatct tgaagagcgc aaagggggtc
     5641 acgtagccgt gggaggagta ctcctggatc tccttggaca tgatcatccc cggcaagagc
     5701 gcccctcgga gcatcacgca catcacggag ctctcgatcc gctcatccac gtccgggatc
     5761 ttgtgcacgg cgtagttctg cacgggcatc cgcacgggcg tccgcaccac gatgtggttc
     5821 ttcttctcgt gggaggcgga gccaaaggcg aagttgaaga gaagctcgta ggcgcgctgg
     5881 gtgtagtaga cgaactcggg ctgcgcggtc cgctcgtcgc gaagctttgc gatgagccgg
     5941 gatacctccc catgcacctc ccgcggcgtc tggaacgggt acacgtggat gcgcggctcc
     6001 cgatccacga tctcccgcat cacccgcccc aggtcgttgt agaggtcgaa cagctccgcc
     6061 atgacccggt cgtgctgggc cgagcggccg ggatcggcga gcgtcgcgaa gcgctccagc
     6121 gcggcggaga accgggcgtc cacctcgtcc aacgtagccc ggtccgcctc cttcagataa
     6181 ccgtccaggt cgtgagcctt caggatgacc ttctccatga ccgccctccc tcgcgctgct
     6241 accgtatcca cgccggtcgg ggtggtcaat gtggcttcgg cgcggtacgc tgagatccgg
     6301 tgctgagaaa gcgataccgg ttccgcggca cggttcaggg cgtgggattc agacccgcca
     6361 tcagccggat tgcgtccggg ctgggtctcg acgggttcgt ccaaaaccgc agctcggagg
     6421 tcgtcgccga ggtccgaggc acggaagaag cgatctcgag gttcgaggcg gccctccaga
     6481 ccggcctgcc cccggcagcc cggctggaga ggatccaggc agaggtcgtc gaatcacccg
     6541 cggagacctc ggaggcgctc gcggaaagcg acggtttcct catcctggag agcgagccgg
     6601 acctctattc attccccccg atcccaccgg acctgccctt gtgcccggag tgcgcggcgg
     6661 agctcctgga ccccggaaac cggaggtacc tgtacccgtt catcacgtgc acccagtgcg
     6721 gcccccggta ttccatcgtg gaacggaccc cgttcgaccg acggaacact tcgatgcgcc
     6781 ccttcgagcc gtgcccggcc tgcgcccgcg agtaccgcga cccggtggac cgcaggttcc
     6841 actcccagac caactcgtgt cccacctgtg gccccaggct caccctgctg gacggggccg
     6901 gacgacggct gacgacagat ccgatcccgg cggccatgga tcttcttgcc gacggcggga
     6961 tcgttgcggt ggaggggatt ggcgggttcc accttgccgc tgtccctgcc caggagggcg
     7021 cggtccagag gctccggcga gacaaggaga gagagagaaa acccttcgcc ctgatggtgc
     7081 aggacctggc ggaagcgacg aagatctgcc gcctttcgcc ggcggaggtc gaagccctct
     7141 cttcctctca ggctccgatc gtgattgcgg cgcgcgctcc cgatgcgccg gattggctgc
     7201 gcgcggtctc cgacacggac acgctcggga tcatgctccc ctacacgccc ctccacctgc
     7261 tcctgttccg tcacccggac cggccgccgc ggtaccgctg cctcgtgatg acctcgggaa
     7321 accgagcgcg tgagcccatc atcacggatc cggcggacgc cgttggaaag ctcttggggg
     7381 tggcggacgc gttcctggtg cacgatcggc ggatcgtcct gcggaccgat gactcgatcg
     7441 tccgcgtcgg cga
//
LOCUS       DASRZS010000490        10401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2180265, whole genome shotgun sequence.
ACCESSION   DASRZS010000490 DASRZS010000000
VERSION     DASRZS010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10401
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180265"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VFH83_11970"
     CDS             <1..876
                     /locus_tag="VFH83_11970"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET6487132.1"
                     /translation="ASNTGFGMYAFVGLGNKCDLDELDFLRHFGKDSRVRCLAFYLES
                     LDAGRDFLKEARAVAREKPVVVLKAGRTATAVSAVSSHTGRMAGSSLVASGAFRQFGI
                     LSATDDEELCDAAKALSHLPAARGNRMAVLTPAGGYGVMCTDYIESRIRGVSLAMARL
                     SQETVDRIRAASFPFASCANPVDLTASADDAMFGKSIDALLEDPGVDILICIAFFAPP
                     QISEDLVSEIAARVRGSSKPVLVFTQYGPFTDGYLRRFYEAGVIGFPSIHRTVRAASF
                     LVERAEILAALEESP"
     gene            873..1556
                     /locus_tag="VFH83_11975"
     CDS             873..1556
                     /locus_tag="VFH83_11975"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET6487133.1"
                     /translation="MTATARAWLEALGATGRPDELETKQLLAAHGLRVPQALRVRLDR
                     GPVTADDPGIGFEPPYVVKVCSAEILHKTDQGGVRLGVDRRSLPSVVSELQARFPGSD
                     LLVEEQVRFAGTEFIIGAFRDPSFGPALMVGAGGILTELYQDVAFRLVPCSTAEAMRM
                     LRELKVFPVLEGFRGRSMDARALAEAVGRVGEIVEALGDRFEQLDVNPIVNGPSGWTA
                     LDAKLILRS"
     gene            complement(1572..4454)
                     /locus_tag="VFH83_11980"
     CDS             complement(1572..4454)
                     /locus_tag="VFH83_11980"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET6487134.1"
                     /translation="MKAEVIPAEENLIDQVALRIEERFPNYGAGSRASRDLSRALVVF
                     PGKRPAHFLRRRLSRTRRGGFLPPRVLSMDELVDGVFCARTSGTFPSVEPIDAVAVLY
                     DSQLAAPHPLGGSSFMTLDAFFPLGLKIYNDLEELLIEGVNHRQVAGAQPLIEEEVPP
                     RSRQSLQTLAWFYQEFYPAVERRGLSTRSQRYRRVAAQIEPPDLEPLDLLILAGFFTL
                     TGAEVELFRKVAEWPQTRLLFQDGPGLARRLSALGIPGLSESSRPPRPRTFFRTSPDT
                     HGQVFALNAALGEPEESTLVVLPDPQTLFPLLRHGLSRFTEDQYNVSLGYSLQRTPLY
                     GFFADLMELIASMEGDRVYLSAYLSFVLHPYTKNIRFRGSAEATRVLFHTLEDRLARV
                     RGRKFSTLREIESDDELFQDAARSVAALGGPAASSSSSRDPELSSSLRAHLARIHGET
                     IEPFRSFRDVGDFAERCIRLISWVHESSTARDHPLFSPFSEAFVRSLETIGRSLMTAK
                     SFTDTASYFALLRRYLQTCYVPFEGTPLHGMQVLGALETRGLHFRRVFVLDATEGTFP
                     PAGSESTLLPLPVRAALKLSSYRDQEDIAAYHFEVLAAGAEELHLFSVEGADRSRSRF
                     VERLLWERQQAEGVAEESSLIDTVSYRVNLENRAPDPVEKTGPMAEWLARFRYSATAL
                     DTYLRCPLRFYYRYLLNLGRREEMTSEIEPADVGLFVHAVLARYFEPRRGRPLTRADA
                     DGVAMEAVVDALFAERFGSSETGAERLLHQQVRSHLKAFVDRYLAELISSHAVSVEGL
                     EASVETAREGHNLYGRLDLVLRRDGAPCLVDFKTSANRPAHQIRLKRLVLEDRATWPV
                     AIPTLQLAFYVLLYSAQNGIPPTDVRALFLLLGRNELDSKIEVPLFESTQAAQDGWPV
                     LEEVILRLLREINNLSVPFTPTPDLKSACPSCDFTGICGTAWLARG"
     gene            complement(4451..7522)
                     /locus_tag="VFH83_11985"
     CDS             complement(4451..7522)
                     /locus_tag="VFH83_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET6487135.1"
                     /translation="MAELEQDTRIRFPSVTVVRASAGSGKTHALVTRCVQLLLSAVIP
                     RNGLRNILAITFSNNAAREMRERVLQTLKRIALGERQSVQEICQVVSGGEERAVHRAA
                     ELVDEILLRYSDFQVRTIDSFMATVLRASAIDFGFSPEFEILLETGDLFDYAFNLFLR
                     GARRGSSRARLLDETVGTVISLRGAEESYPWDPAPLLLTQLKGIEAELASLEEPPCCT
                     DGVERMQEAERGVRAIFEEVEGRVRASGLEPNGNSTFPDLLAAVRAGRTATLIGRGTR
                     TPPVRKPPARDLAGLERYQRIVETWGLARTRVAQYVSAWARAFYQPYLHLHEEMAPTL
                     AQAKRFNGVVFISDITRSLSAYLADDTVPDVYFRIGERVFHHLIDEFQDTSPTQWRSL
                     FPLVENSLAGGGSLFAVGDTKQAIYGFRRADYTIMRRLETENPFPSAEHGVQTLGHNY
                     RSRPRLIELASRVFTTNAAREYPEAASRSGLADWRQVAADTREPGHAEVSILTRDDDQ
                     PPERERLRGILADLRDRGYRWEDIAVLAARNQDIIRAASWLSELGVPFVSFSSLDVRG
                     RRISSEMLALLSFLDSPPDDLAFATFLLGRIFDRAVRRYGGASGDSLRGFLFRSRSER
                     PLYKAFQEAFPDLWRSCFAGLFRSAGYLPLYDLVCEAYTAFDVFRAAGDEEATLAKLL
                     EVVKDFEGSGTNSLREFLEASHSDSADWAITEPMSLPSVQLMTIHKAKGLGFPVVIAL
                     LYGESRRGSRYAILREPEGVDMVRLTRETAEADPDMEALYEEEVIRDRVERLNGLYVA
                     LTRARHELYVVGVKAERDTYPFDVLPEDGYATPGPKPRAHPPAAAGVEAERPTGAPLA
                     FTVRPTGETAGRARSSLSREERLRGELIHRMLALVPFAEGDVAAALETAARRAATETR
                     QPPELSSARAAARLIETTELGALFARAPGREVLTEQEFCDATGRLYRMDRVVVDPERV
                     TVVDYKTGTVVHPEHLEETRGYAGILAGIYPGRRVEAILAYLDAGRLRRVV"
     gene            7591..8532
                     /locus_tag="VFH83_11990"
     CDS             7591..8532
                     /locus_tag="VFH83_11990"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HET6487136.1"
                     /translation="MKARIRVGACAAGALLLLGSCVSAPTSAPNAAQPALQAPDGTGT
                     VDFATIERLSEEYRVMRYKSQPDIRAELARYYDPFVLVDLPSTRNRYVLGTVRGEHRQ
                     EILIRGTANGRNVLYDLQFARRYDQRLGIDLHTGFDAMALALYADIRPRLDKSDDLVI
                     FGHSLGAAEAVILSMLLSQEGFRIVMVYASGGPRVTDRAGEAKYDTLPILRIINQGDP
                     VPLLPPKDTPNPEDPYVHIGPAVLLLDGPAYCLLRDDVGDDAFASTFWKFLGDRSVRH
                     DVQEHLIPAYIDRLKPKLTEAIQVPFADRLKYVAAAK"
     gene            complement(8569..9876)
                     /gene="hemL"
                     /locus_tag="VFH83_11995"
     CDS             complement(8569..9876)
                     /gene="hemL"
                     /locus_tag="VFH83_11995"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET6487137.1"
                     /translation="MGRSLNTKRSDALYAQALTLLPGGVDSPVRAFKAVGGGPLFIAR
                     GEGPYLHDVDGNRFIDYVLSWGPLALGHAHPAVVTALQEAVARGTSFGAPSPLEIDLA
                     LLIQRHVPSIEVIRFVSSGTEAAMSALRLARAHTGRTKIVKFVGGYHGHADLLLVQAG
                     SGVATLGLPDSPGVPAAVTADTLSCSYNDLDGVKALFARYPRQIAAIIVEPVAGNMGV
                     VLPDPAFLPGLREIASEDGALLVFDEVMSGFRVHLGGAQARYSVRPDLTVLGKVIGGG
                     LPVGAYGGRAEIMGKIAPAGPVYQAGTLSGNPLAMTAGIATLTELGRDGVWAGLERTA
                     EDLAAGLAEAARAAGVDVQVNRAGSMLSLFFAREPVTDWERAKRSNLEQFAHYFRGML
                     EAGVYLAPSQFEACFLSTVHGAKEVSRTLEVAERAFRSVASAR"
     gene            complement(9887..>10401)
                     /locus_tag="VFH83_12000"
     CDS             complement(9887..>10401)
                     /locus_tag="VFH83_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6487138.1"
                     /translation="ELGALLAGIPAASPAIVHLGYAASSVERALDAYGYVIPRVEGSP
                     FIACTWTSSKWEGRAPQGMVLLRLYAGRAGGPEPGSNEDLLLAAREELAETLGIRAAP
                     VLTRIHRWERGMPQYTLEHPRRRSAIGTRLEEIPGLYLAGASYRGVGIPDCIESGAEA
                     ARAAIHQLAR"
BASE COUNT         1721 a         3514 c         3538 g         1628 t
ORIGIN      
        1 gccagcaaca ccggcttcgg catgtacgca tttgtcggcc ttgggaacaa gtgcgatctg
       61 gacgagctgg acttcctccg gcacttcggc aaggacagcc gcgtgcggtg cctggccttc
      121 tacctggaga gcctggacgc gggccgggac ttcctgaagg aggcgcgcgc ggttgcccgg
      181 gagaagcccg ttgtggtgct caaggcaggg cggacggcaa cggccgtctc cgcggtcagc
      241 tcccataccg ggcgcatggc cggctccagc ctcgtggcca gcggcgcgtt tcgccagttc
      301 gggatccttt ccgccacgga cgacgaggag ctgtgtgacg cagccaaggc gctctcccac
      361 ctccctgccg cccgcgggaa ccggatggcg gtcctcacgc cggccggggg ctacggagtg
      421 atgtgcaccg actacatcga gagccgaatc cgcggggtca gcctggccat ggcgcgcctg
      481 agccaggaga ccgtggatcg cattcgagcc gcctccttcc cgttcgcctc ctgcgcgaat
      541 cccgtggacc tcacggcgag cgccgatgac gccatgttcg gcaagagcat cgacgccctc
      601 ctggaggatc cgggcgtgga catcctgatc tgcatcgcct tcttcgctcc gcctcagatc
      661 agcgaagacc tggtctcgga gatcgcggcc cgggtccggg ggtcctccaa gccggtcctg
      721 gtgttcacgc agtatgggcc gttcaccgac ggctatctgc ggcggttcta cgaggccggc
      781 gtgatcggat tcccttccat acaccgcacc gttcgggcgg cgagcttcct cgtggagcgg
      841 gcggagatcc tggccgcgct ggaggagtcg ccatgaccgc gaccgcgcgg gcctggctcg
      901 aagcccttgg cgcgaccggt cggccggatg agctggagac caagcagctt ctggcggcgc
      961 acggcctgcg ggtaccccag gccctgcggg tccggctcga ccgtggaccc gtcaccgcgg
     1021 atgatccagg catcgggttc gagccgccct acgtcgtgaa ggtctgctcc gccgagatcc
     1081 tccacaagac cgaccagggc ggcgtgcggc tcggggtgga ccggcggtct ctgccttccg
     1141 tggtcagcga gctacaggcc cggttcccgg ggagcgacct gctggtggag gagcaggtcc
     1201 ggttcgccgg caccgagttc atcatcggcg ccttccgcga tccatccttc gggccggccc
     1261 tgatggtcgg tgccggaggc attctcaccg agctctacca ggacgtggcg ttccggctcg
     1321 ttccctgctc gacggcggaa gccatgcgca tgcttcggga gctcaaggtg ttccccgttc
     1381 tcgagggttt ccgaggacgg tcgatggatg ctcgggccct ggcggaggcg gtcggccggg
     1441 tcggggagat cgtggaagcg ctgggggatc ggttcgagca gctcgacgtg aatccgatcg
     1501 tcaacggtcc ctccgggtgg acggcactgg acgcgaagct gattctgagg tcctgagcct
     1561 ggcgggcccg gctaccctcg cgccagccac gcggtcccgc agatgcccgt gaagtcacag
     1621 ctcggacagg ccgacttgag gtccggcgtc ggggtgaacg ggaccgagag gttgttgatc
     1681 tcccgcagca gcctcaggat cacctcttcc aggaccggcc agccgtcctg cgccgcctgg
     1741 gtgctttcga agagcggcac ctcgatcttg ctgtccagct cgttcctgcc gaggagcagg
     1801 aacaacgcgc ggacgtcggt cggcgggatg ccgttctggg ctgaatacag gagcacgtag
     1861 aaagcaagct ggagcgtagg gattgccacc ggccaggtcg cccggtcctc caggaccagc
     1921 cgcttcagcc ggatctggtg agccggtcga ttggcggatg tcttgaagtc caccaggcac
     1981 ggcgcgccgt cccgccgaag cacaaggtcc agcctcccgt acaggttgtg gccctcgcga
     2041 gccgtctcga cggaagcctc cagaccctcc acggagacgg cgtgggagga gatgagctcg
     2101 gcgaggtagc ggtcgacgaa cgccttcaga tgggatcgga cctgctggtg cagcagtcgc
     2161 tccgctccgg tttcggagga gccgaaccgc tcggcgaaca gggcatccac cacggcctcc
     2221 atggcaacgc catcggcgtc cgccctcgtc aacgggcgtc cccgcctcgg ctcgaagtag
     2281 cgggccagga cggcgtggac gaacagcccc acgtcggcgg gctcgatctc cgaggtcatc
     2341 tcctcccgtc gacccaggtt gaggaggtaa cggtagtaga accgcagcgg gcagcgcagg
     2401 taggtatcca acgccgtggc gctgtagcgg aaccgggcga gccactccgc catgggcccc
     2461 gtcttctcaa cgggatccgg ggctcgattc tccaggttca cccgatagga gacggtgtcg
     2521 atcaggctcg attcctccgc gaccccctcc gcctgctgac gctcccagag gagccgctcg
     2581 acgaaccggg atcgggatcg gtccgcaccc tcgacggaga agaggtgcag ctcctccgcg
     2641 ccggccgcaa ggacctcgaa gtggtaggcg gcgatgtctt cctggtcgcg gtagctggag
     2701 agcttcagcg ccgcccgtac ggggagggga agcagcgtgc tctccgagcc cgcgggcggg
     2761 aaggtgcctt cggtggcgtc caggacgaac acgcggcgga agtggagccc gcgggtctcc
     2821 agggcgccga gtacctgcat cccatgcagc ggtgtgccct cgaacggcac gtagcaggtc
     2881 tgcaggtatc ggcgcagaag ggcgaagtag ctggcggtgt ccgtgaagct ctttgccgtc
     2941 atgagggatc gaccgatggt ctccagggat cggacgaacg cctccgagaa cggagagaac
     3001 aggggatggt cccgcgccgt gctggactcg tgcacccagg agatgagtcg gatgcaccgt
     3061 tcggcgaaat cccccacgtc ccggaaggac cgaaacggct cgatggtctc cccgtggatg
     3121 cgcgccagat gggcgcggag gctggatgaa agctcgggat cccgggaaga ggaagaagag
     3181 gcggcaggcc cgcccaacgc ggccacgcta cgcgccgcgt cctggaacag ctcatcgtcg
     3241 gactcgatct cccgaagcgt ggagaacttc cttccccgga cccgagccag ccggtcctcc
     3301 agcgtgtgga agaggacccg ggtcgcctcc gcggatccgc gaaaccggat gttcttggta
     3361 tacgggtgga gcacgaacga caggtaagcc gagaggtaga cccggtctcc ctccatggag
     3421 gcgatcagct ccatcaagtc cgcgaagaac ccgtacaacg gcgtccgctg gagggaatag
     3481 ccgagggaga cgttgtactg gtcctcggtg aaccgtgaca gaccgtgccg caggagggga
     3541 aagagagtct gcgggtccgg caggaccacc agcgtggact cttcgggctc gcccagcgcc
     3601 gcgttcagcg cgaacacctg gccgtgcgtg tcggggctgg ttcggaagaa ggtcctggga
     3661 cgcggagggc ggctcgactc gctcagcccc ggtatcccca gggctgacag gcgtcgcgcc
     3721 aggccggggc catcctggaa caggagccgg gtctggggcc actccgcgac ctttcgaaag
     3781 agctcgactt ccgcgccggt cagcgtgaag aacccggcca ggatgaggag gtccaggggc
     3841 tcgaggtctg gaggctcgat ctgggccgcc acccggcggt agcgctggga ccgggtcgaa
     3901 aggccgcggc gttccacggc cggatagaac tcctggtaga accacgccag cgtctgcagg
     3961 ctctgacggg atcgaggagg gacctcctct tcgatgagag gctgggctcc cgccacctgt
     4021 cggtggttga ccccttcgat caacagctct tcgaggtcgt tgtagatctt gagtccgaga
     4081 ggaaagaacg catccagggt catgaacgag cttccaccca acggatgcgg cgcggcgagc
     4141 tggctgtcgt agaggacggc aacggcatcg atcggctcga cactcgggaa tgtgccggaa
     4201 gtccttgcgc agaaaacgcc atccaccagc tcgtccatcg agagcacgcg gggagggaga
     4261 aagcctccac ggcgcgttcg ggaaagccga cgtctcagga aatgggccgg acgcttgccg
     4321 gggaacacga cgagcgcgcg ggagaggtcc cggctggcgc gggaccccgc gccgtagttc
     4381 gggaaccgct cctcgatgcg aagggcgacc tgatcgatga ggttttcctc ggccgggatc
     4441 acttcggctt tcacaccacc ctccgaagcc tgccggcatc caggtacgcc aggattgcct
     4501 ctacccgacg cccgggatag atgccggcca ggatgcccgc gtaccctcgc gtttcctcga
     4561 ggtgctcggg gtgcaccacg gtacccgtct tgtagtccac cacggtcacc cgctcggggt
     4621 ccacgaccac gcggtccatc cgatacagcc ggccagtggc gtcacagaac tcctgttcgg
     4681 tgagcacctc acgcccgggt gcccgggcaa acagggcgcc cagctcggtg gtctcgatca
     4741 accgggcggc cgcgcgggcg ctcgagagct ccggcggctg gcgcgtctcg gtggccgccc
     4801 gacgggccgc cgtttcgagc gccgcggcca cgtcgccctc cgcgaagggg accagggcga
     4861 gcatgcggtg gatcagctcg ccgcgaaggc gctcctcgcg gctcaggctg gacctggcgc
     4921 gcccggccgt ctcccccgtg ggtcggaccg tgaacgccag gggcgccccg gtaggcctct
     4981 cggcctccac gccggccgcg gcgggtggat gcgctcgcgg tttcggacct ggcgttgcgt
     5041 agccgtcttc gggcaatacg tcgaacggat aggtgtctcg ctccgccttc acccctacga
     5101 cgtacagctc gtgtcgggct cgggtcagcg cgacatacag gccgttgagc cgttccaccc
     5161 ggtcgcggat cacctcctcc tcgtaaagcg cctccatatc cggatccgcc tcggcggtct
     5221 cccgggtgag ccgcaccatg tccacgccct ccggctcccg caggatggcg taccgggacc
     5281 cccgccggga ctccccatag aggagcgcga tcacgaccgg aaacccgagg cccttggcct
     5341 tgtggatcgt catgagctgc acggagggca ggctcatcgg ctccgtgatg gcccagtccg
     5401 cgctgtccga gtgggaggct tccaggaact cccgaaggct gttggttccc gacccttcga
     5461 agtccttcac cacctcgagg agcttggcca gggtcgcctc ttcgtcgccg gccgcgcgga
     5521 acacgtcgaa ggcggtgtac gcttcgcaca ccaggtcata cagcggcagg taaccggcgc
     5581 tcctaaagag gccggcgaag caggacctcc agaggtccgg gaatgcctcc tggaatgcct
     5641 tgtacagcgg acgctccgat cgggaccgaa agagaaagcc gcggagggag tctcccgatg
     5701 cccccccgta gcggcggacg gcccggtcga agatccgacc cagcaggaac gtggcaaagg
     5761 cgaggtcatc cggcggcgaa tccaggaagg agagcagcgc cagcatttcc gaggagatcc
     5821 gtcggccgcg gacgtccagg ctgctgaagg agacaaaagg gactcccagc tcgctgagcc
     5881 agctcgcggc gcgaatgata tcctggttgc gggcagccag cacggcgatg tcctcccaac
     5941 ggtacccgcg atcgcgcagg tcggcgagga tgcctcgcag tctctcgcgc tccggcggct
     6001 gatcgtcgtc ccgagtcagg atgctcacct ccgcgtgtcc cggctcccgc gtgtccgccg
     6061 ccacctgccg ccagtcggcc agcccgctcc gtgaggcagc ctccggatac tcccgcgcgg
     6121 cgttggtcgt gaacacgcgg gaggccagct cgatcagccg gggccggctg cggtagttgt
     6181 ggcccagggt ctgcacaccg tgttccgcgg acgggaacgg gttttccgtc tccagcctgc
     6241 gcatgatggt gtagtccgcc cttcggaacc cgtagatggc ctgcttggtg tcgcctaccg
     6301 cgaacaggct ccctccgccg gccagggagt tctccacgag agggaagagg ctccgccatt
     6361 gggtgggaga ggtgtcctgg aactcgtcga tcaggtggtg gaacacccgc tcgccgatcc
     6421 ggaagtacac gtccggcacc gtgtcatcgg ccaggtacgc ggagagggac cgggtgatgt
     6481 cggagatgaa gaccacgccg ttgaaccgct tggcctgggc cagggtcggg gccatctcct
     6541 cgtggaggtg caggtagggc tgatagaacg ctcgcgccca cgccgagacg tattgggcca
     6601 cccgggtcct ggccagaccc caggtctcga cgatccgctg gtagcgctcc aggcccgcca
     6661 ggtccctggc gggcggcttg cggaccggcg gggtccgtgt gccccggccg atgagggtcg
     6721 cggtccgacc cgcgcgcacg gcggccagca gatcgggaaa cgtggagttg ccgttgggtt
     6781 ccagccccga cgctcgtacc cgtccctcca cctcctcgaa gatggcccga acgccgcgtt
     6841 cggcctcctg catccgctcc acgccgtcgg tgcagcaggg cggctcctcc agggaggcga
     6901 gctcggcctc gatgcccttg agctgggtca ggagcagagg agccggatcc cacgggtagg
     6961 actcctccgc gcctcgaagc gagatcaccg tgccgacggt ctcatccagg aggcgggcgc
     7021 gtgagcttcc gcgtcgggcg ccgcgcagga agaggttgaa ggcgtagtcg aagaggtcgc
     7081 ccgtttccag gaggatctcg aactccggac tgaacccgaa gtcgatggcc gaggcgcgca
     7141 ggaccgttgc catgaagctg tcgatggtcc ggacctggaa gtccgaatag cgcaggagga
     7201 tctcgtcgac cagctcggcg gcccggtgga ccgctcgctc ttctccgccc gagaccacct
     7261 ggcagatctc ctggacgctc tgccgttccc ccaacgcgat ccgcttgagc gtctggagca
     7321 cccgctcccg catctcccgg gccgcgttgt tggagaaggt gattgccagg atgttgcgca
     7381 gtccgttgcg cgggatgacc gcggagagca ggagctggac gcaccgcgtg accagggcgt
     7441 gggtcttgcc ggaccccgcg gacgcccgga ccaccgtcac gctgggaaac cggatccggg
     7501 tgtcttgctc gagctcagcc atccgccgcc atgatagccg gggagtcgca aacttgacag
     7561 ggtacgcccg cagagcctac aacccgtggc atgaaagccc gtatccgcgt aggcgcgtgt
     7621 gccgcgggag ccctgctgct cctgggctct tgcgtgagcg ctccgaccag cgcgccgaac
     7681 gcggcccagc ccgcgctcca ggcgccggac ggcaccggga ccgtggactt cgccaccatc
     7741 gagaggctct ccgaggagta ccgggtgatg cgctacaaga gccagccgga catccgggcg
     7801 gagctggcgc ggtattacga tcccttcgtc ctggtggatc ttccgagcac gcggaaccgc
     7861 tacgtgttgg ggacggtccg cggagagcat cgccaggaga tcctcattcg cgggaccgcc
     7921 aacggcagga acgtcctgta cgacctgcag ttcgccaggc gatacgacca gcggcttggg
     7981 atcgacctgc acacggggtt cgatgcgatg gccctggccc tctatgccga catccgtccg
     8041 cggctggaca agtctgacga cctggtgatc ttcggtcact ccctgggcgc ggcggaggcg
     8101 gtcatcctct ccatgcttct ctcccaggaa gggtttcgca tcgtgatggt ctacgcctcc
     8161 ggtgggccgc gggtcaccga ccgggcagga gaggcgaagt acgatacgct tccgatcctc
     8221 cgcatcatca accagggaga tcccgttccg ctcctgcccc ccaaggacac tccgaatccc
     8281 gaggatccct acgtgcacat cgggcccgcg gtactgctcc tggatggacc ggcctactgt
     8341 ctgctgcggg atgacgtcgg cgatgacgcc ttcgcatcca cgttctggaa gttcctgggg
     8401 gatcgaagcg tccgccacga cgtccaggaa cacctcatcc ccgcgtacat cgaccggctc
     8461 aagcccaagc tcacggaggc gatccaggta ccctttgcgg atcggctgaa gtacgtggca
     8521 gccgcgaagt agccatcgcc cgtcgaccgg ttatcgcccg gtgaccagtc atcgcgccga
     8581 ggccacggac cgaaacgctc gctccgccac ctccagggtg cgggatactt ccttggctcc
     8641 atggaccgtc gacaggaagc acgcctcgaa ctgggacggc gccaggtata cgccggcctc
     8701 cagcataccc cggaagtagt gggcaaactg ttcgaggttc gatcgcttgg cgcgctccca
     8761 gtccgtgacc ggctcgcgcg cgaagaagag gctcaacatg gaccctgccc ggttcacctg
     8821 gacgtccacg cctgccgcgc gcgcggcctc ggcgagaccc gcggccaggt cctccgcggt
     8881 tcgttccagg ccggcccaca ccccatcccg acccaactcg gtcaacgtgg cgatgccagc
     8941 ggtcatggca agaggattgc cagacagggt tcctgcctgg tacacgggac cggccggagc
     9001 gatcttgccc atgatctcgg ccctgccgcc gtaggcgccg accggaagcc cgcctccgat
     9061 caccttgcca agcacggtga ggtccggccg aaccgagtac cgggcctggg ccccgcccag
     9121 gtggacccgg aacccgctca tcacctcgtc gaacaccagc agggcgccat cctccgacgc
     9181 gatctcccgc agcccgggca ggaatgccgg gtcgggcaga acgacgccca tgttgcccgc
     9241 caccggctcc acgatgatcg ccgcgatctg tcggggatat cgcgcgaaga gggccttcac
     9301 gccgtccagg tcgttgtagg agcaggacag tgtatccgcg gtcaccgcag ccgggactcc
     9361 cggagaatcc ggcagaccca acgtggcgac tccggatccc gcctgcacca ggagcaggtc
     9421 cgcgtggccg tggtacccgc ctacgaactt gacgatcttg gtgcggccgg tgtgcgcccg
     9481 agccagccgg agcgcgctca tggcagcctc cgtgccggag ctcacgaacc ggatcacctc
     9541 gatgctgggg acgtggcgct ggatcagtag cgcaagatcg atctccaggg gagacggcgc
     9601 gccgaagctg gtgccgcgag cgacggcctc ctggagcgcc gtgacgaccg ccggatgggc
     9661 atggcccaga gccaagggac cccacgacag gacgtagtcg atgaaccggt tcccatcgac
     9721 gtcgtgaagg tagggcccct ccccgcgggc tatgaagagc ggtcccccgc ctaccgcctt
     9781 gaacgcgcgg accggcgaat ccacgccacc gggcagcagc gtgagggcct gagcatagag
     9841 cgcgtcggat ctcttcgtgt tcaacgatct tcccatggtc gttcctctat cgcgcaagct
     9901 gatggatggc ggcccgagcg gcctccgctc cggactcgat gcagtccgga atgccgactc
     9961 ctcggtacga cgcgcccgcg aggtagaggc cggggatctc ctccagccgc gtcccaatgg
    10021 ccgatcgtct tcgcgggtgt tccagggtgt actgcggcat gccgcgctcc caacggtgga
    10081 tcctggtgag caccggtgcg gcgcgaatcc cgagggtctc ggcaagctcc tcccgagcag
    10141 ccagcagcag atcctcattc gaacccggtt cgggtcctcc cgcccgaccc gcgtacagcc
    10201 gaaggaggac catcccctgc ggcgcccggc cttcccactt gctggaggtc caggtacagg
    10261 cgatgaaagg gctcccctcg acccggggaa tcacgtagcc gtaagcgtcc agcgctcgtt
    10321 ccacggatga cgccgcgtag ccgaggtgca cgatggcggg agaggccgcc gggatgccgg
    10381 ccagaagcgc gccgagctcc a
//
LOCUS       DASRZS010000491         4507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5269978, whole genome shotgun sequence.
ACCESSION   DASRZS010000491 DASRZS010000000
VERSION     DASRZS010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5269978"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VFH83_12005"
     CDS             complement(<1..754)
                     /locus_tag="VFH83_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HET6487139.1"
                     /translation="MHNPRTAVIVIPTFNEASSIGMLLDELVHTVLPATPWDCRVLVV
                     DGNSPDGTAEIVRKASRECAGVHLLVEEKKEGLGAAYVKGFRHAVAELKADAVVEFDG
                     DLQHPPEVIPRLLAEIDAGADLVLGSRRRPGGSYPKGWSPLRRFLSSVGGFAARLLLF
                     FPSRLFRAVTDPTTGLKATRVDERFLALDLSALSRGFAYKLEMLSLLAGAGAVIREVP
                     LAFRLRGGGESKLEGQAPWEILWTCILLRLRQP"
     gene            823..2187
                     /locus_tag="VFH83_12010"
     CDS             823..2187
                     /locus_tag="VFH83_12010"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET6487140.1"
                     /translation="MRSARRPRQFFLLVVDVAMFLLALLGALLIRFRAVPGGELWWSL
                     VQTFIPSWILWIVTLYTADLYNIESAFDDVRFAGRLFAAVVVCGLISALMFYLARFSQ
                     TPKTILAIYVAMLAVLLWLWRFAYGRISRLYLPRRVTAFVGVDSTVEELVSVIETSDH
                     LGYEAAAFLDEAGSAPASLRARSCRTCAEFVEVVGRRGVSEVVVADEGRLSEATRRSL
                     LGLIERRVRFIRLPDFYENYLRKVPLGTINDLWFLENIDLSGKARYRIAKRILDIFIS
                     LVFLAAFFVPGLLVAGGIAVSSRGPVFFRQQRLGVGGRPFWILKFRTMRVDGNTFVPT
                     AERDPRVTGFGRFLRRFNLDEIPQAINILVGEMSLIGPRPERPELVQDLEQSIPYYRQ
                     RLLVKPGITGWDQVSGEYHSPSVEDTYKKLQYDLYYVKNMSPLLDISIFFKSITTMLG
                     RKGR"
     gene            2286..3317
                     /locus_tag="VFH83_12015"
     CDS             2286..3317
                     /locus_tag="VFH83_12015"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HET6487141.1"
                     /translation="MQASQSRYGTTSDGRAVTEFVLESARGARVQIIDLGATVTSVMI
                     PDRAGKSENVCLGHGNLSGWEKNDTFFGCIVGRVANRIRDGRFDLDGKPYSVARNVLG
                     KHHLHGGIKGFDKYVLKAKLFRRGNAAGVRFSHTSPDGDEGYPGTLKLTAEYVLTDAN
                     ELTFEYWATADKATPVNITNHAYWNLAGARAGESGTVLAHEARFSCPFYLPVDGDLMP
                     TGEIHKTAGSAFDFSEFKSIGKDIASVPGGGYDHCLVVGKRSGEMGLIGTVRDPASGR
                     AMEVHTTMPGVQFYTGNFLDGSLFPKHGGFCLETQWFPDSPNIGHFPNAILRPGQTYH
                     HRTVHKFSS"
     gene            3330..>4507
                     /locus_tag="VFH83_12020"
     CDS             3330..>4507
                     /locus_tag="VFH83_12020"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HET6487142.1"
                     /translation="MLFNSLDYFVFLPIVIGLYIAVPRPFRWLLILCAGYFFYAYWRV
                     EYTLLIVFSTLVDYTAGRVMGASSSAQTRKVILLVSLGINIGLLSFFKYFNFLSDSIG
                     WLLTASGTHVLVPHRAIVLPVGLSFYTFQSMAYTIEVYRRKVEPETHLGRYAEYVVFF
                     PQLVAGPIERPSHLLPQLRGAFDITWENLSAGVQRILLGIFKKVVVADRLALFVNNVY
                     ASPQQFNGPPLFLATLFFAVQIYCDFSGYSDIAIGSARIFGVRLIENFERPYLSKSIG
                     EFWSRWHIALSTWFRDYVYIPLGGNRAARPRVLFNLFVTFLISGLWHGASWTFVIWGA
                     YHGLLVSLERLLPVLGRTPRTLAMKLVRGAMSFALVLVGWVFFRARSLGDALFILGHL
                     G"
BASE COUNT          714 a         1519 c         1421 g          853 t
ORIGIN      
        1 ccggctggcg gagcctcagg aggatgcacg tccagaggat ttcccaggga gcctgcccct
       61 ccagcttgga ttccccgccc cctcggagcc ggaaggcgag cggcacctcc cggatcacgg
      121 cgccggcgcc ggccagcagc gagagcatct ccagcttgta ggcaaacccg cggctcaggg
      181 ccgagaggtc gagcgcgagg aagcgctcgt ccacccgtgt ggccttgagg ccggtggtgg
      241 ggtccgtgac cgctcggaac agccgtgacg gaaagaacag cagcagccga gccgcgaacc
      301 cgcccaccga gctgagaaag cgccgcagcg ggctccaacc cttcggatag gaaccgccgg
      361 gcctccgtcg gctgcccagc acgaggtccg cgccggcgtc gatttccgca aggagccggg
      421 gaatgacctc cggcggatgc tggaggtctc catcgaactc caccacggca tccgccttga
      481 gctccgcaac cgcgtggcgg aaccccttga cgtaggccgc tcccaacccc tccttcttct
      541 cctccaccag caggtgcact cccgcacatt cccgagaagc ctttctcacg atctctgccg
      601 taccgtccgg cgaattgccg tctaccacga gcacgcggca gtcccagggc gtggccggca
      661 gcacggtgtg gaccagctcg tccaggagca tgccgatgga gctcgcctcg ttgaaggtcg
      721 ggatgacgat gactgcggtc cggggattgt gcaccgtgct acccggtact ccgggacacc
      781 ggggggtgtc aagctgccgg gcgtggtggt agtatcccgc tgatgcggtc cgcgcgcaga
      841 ccccgccagt tctttctcct cgtggtggac gtcgccatgt tcctcctggc cctgctcggt
      901 gcgctgctga tccgcttccg cgccgttccg ggcggggagc tgtggtggtc tctcgtgcag
      961 actttcatcc cgtcatggat cctgtggatc gtcacgctgt acacggcgga cctctacaac
     1021 atcgagtccg cgttcgacga tgtccggttc gccgggcgcc tgttcgccgc ggtcgtggtg
     1081 tgcgggctca tttcggctct catgttctac ctggccaggt tcagccagac gccgaagaca
     1141 atcctggcga tctatgttgc gatgctcgcc gtgctgctgt ggctctggcg gtttgcgtac
     1201 ggaaggatct cccgtctcta tctcccccgg cgggtcacgg cctttgtcgg ggtggattcc
     1261 acggtggagg agctggtgag cgtgatcgag acctccgacc acctggggta cgaggcggcc
     1321 gcgttcctgg acgaggcggg cagcgctccg gcgagcctcc gggcacggag ctgccgcacg
     1381 tgcgccgagt tcgtggaggt ggtcggccgc cgcggcgttt ccgaggtggt ggtggccgac
     1441 gagggcaggc tctcggaggc gacccggcgc tccctgctgg ggctcatcga gcggcgggtg
     1501 cggttcattc gcctccccga tttctacgaa aactacctcc gcaaggttcc cctggggacg
     1561 atcaacgacc tgtggttcct ggagaacatc gacctctcgg ggaaggcgcg ctaccgcatc
     1621 gccaagcgca tcctggacat cttcatctcg ctcgtgtttc tggcggcgtt cttcgttccg
     1681 ggcctgctcg tggccggcgg catcgcggta tccagccgcg gaccggtctt cttccgccag
     1741 cagcgcctgg gcgtgggcgg gcgcccgttc tggatcctga agttccgcac catgcgggtg
     1801 gacggaaaca ccttcgtgcc caccgcggag agggatccgc gcgtgacggg cttcgggcgc
     1861 ttcctgcgca ggttcaacct ggatgagatc ccgcaggcca tcaacatcct ggtcggcgag
     1921 atgagcctca tcggcccacg tcccgagcgc cccgagctgg tccaggacct ggagcagagc
     1981 atcccgtact accggcagcg tctcctggtg aagccgggga tcacggggtg ggaccaggtc
     2041 tcgggggagt accattcgcc gtccgtggaa gacacctaca agaagctgca gtacgacctg
     2101 tactacgtga agaacatgtc ccctttgctg gacatttcga tttttttcaa gagcatcacc
     2161 accatgctgg gacgcaaggg ccggtaggcc cgcacgcccc gcccctgcct cttccccccc
     2221 gcccctttcc cctccgccag atgacgtgca tactcactcc catccagctc gtacgggggg
     2281 aacgcatgca ggcatctcaa tcgcggtacg gcaccacatc cgacggcagg gccgtcaccg
     2341 agttcgtcct ggaaagcgcg cggggagcgc gcgtgcagat catcgacctg ggggccaccg
     2401 tgacctccgt gatgatcccc gaccgcgcgg gaaagtcgga gaacgtgtgc ctgggccacg
     2461 gcaacctgtc cggctgggag aaaaacgaca ccttcttcgg gtgcatcgtc ggcagggtgg
     2521 ccaaccgcat ccgcgacggc cgcttcgatc tggacggcaa gccctactcc gtcgcacgga
     2581 acgtcctcgg caagcaccac ctgcacggcg ggatcaaggg attcgacaag tacgtgctca
     2641 aggcaaagct gttccgcagg ggtaatgcgg ccggggtccg attctcacac accagccccg
     2701 acggtgacga gggctacccg ggaacgctca agctgaccgc agagtacgtg ctcacggacg
     2761 ccaacgagct caccttcgaa tactgggcca cggcggacaa ggccactccc gtgaacatca
     2821 ccaaccacgc ctactggaac ctcgccggcg ccagggcggg ggagtccggc accgtgcttg
     2881 cccacgaggc gcggtttagc tgcccgtttt acctcccagt ggatggcgac ttgatgccca
     2941 ccggcgagat ccacaagacg gccggcagcg cgtttgactt ctccgagttc aagtccattg
     3001 ggaaggacat cgcctcggtc cccggcgggg gctacgacca ctgcctcgtg gtgggcaagc
     3061 gctcggggga gatggggttg atcggaacgg tgcgggaccc ggcaagcgga cgtgccatgg
     3121 aagtgcatac caccatgccg ggcgtgcagt tctacaccgg caacttcctg gacggcagcc
     3181 tcttcccgaa gcacggcggt ttctgcctgg agactcagtg gttccccgac agcccgaaca
     3241 tcggccattt ccccaacgcc atccttcggc ccgggcagac ctaccaccac cgcacggtac
     3301 acaagttctc gagctgatcc ggggcccgga tgctcttcaa ctcgctcgac tactttgtct
     3361 tcctgccgat cgtcattggc ctgtacatcg ccgtcccccg gccgttccga tggctgctga
     3421 tcctctgcgc cggctacttt ttctacgcgt actggcgggt cgagtacacc ctcctgatcg
     3481 tcttctccac gctggtggac tacaccgcgg gccgggtgat gggcgcaagc tcttccgccc
     3541 agacccgcaa ggtcatcctg ctggtcagcc tgggcatcaa catcggcctg ctcagcttct
     3601 tcaagtactt caacttcctc tcggacagca tcggctggct gctgactgcc agtgggaccc
     3661 acgtcctggt cccgcaccgc gccatcgtat tgcccgtggg cctctcgttc tacacgttcc
     3721 agagcatggc gtataccatc gaggtctacc gccggaaagt ggaacccgag acccatctcg
     3781 gccggtacgc ggagtacgtg gtgttctttc cccagctcgt cgccggtccc attgaacgac
     3841 cttcccacct gctgccgcag cttcgggggg ctttcgacat cacgtgggag aacctgtcgg
     3901 ccggggtaca gcgtatcctg ctgggcatct tcaagaaggt cgtcgttgcc gaccgcctgg
     3961 cgctgttcgt gaacaacgtc tatgcttctc cgcagcagtt caacgggccc ccgctgttcc
     4021 tggctacgct gttcttcgcg gtgcagatct actgcgactt ctccggctac agtgacatcg
     4081 ccatcggcag cgcgcggatt ttcggggtac ggttgatcga gaacttcgaa cggccctacc
     4141 tgtcgaagtc catcggggag ttctggtcgc gatggcacat tgccctctcc acctggttcc
     4201 gggactacgt ctacatcccg ctgggtggaa accgggcggc gcgcccgagg gtgctgttca
     4261 acctgttcgt gacatttctc atcagcggct tgtggcacgg ggcgagctgg accttcgtca
     4321 tttggggcgc ctaccacggg ctgctcgtgt ccctggaacg gctgctaccg gtcctgggcc
     4381 gcacgccccg gacgctggca atgaagctcg ttcgaggggc gatgtccttt gccctggtgc
     4441 tcgtgggctg ggtgttcttc agggcgcggt cgctcgggga cgcgctgttc atcctgggcc
     4501 acctcgg
//
LOCUS       DASRZS010000492         2924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4887682, whole genome shotgun sequence.
ACCESSION   DASRZS010000492 DASRZS010000000
VERSION     DASRZS010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4887682"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFH83_12025"
     CDS             complement(<1..392)
                     /locus_tag="VFH83_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014368540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6487143.1"
                     /translation="MRGIPSDSSPEAPASDGSSVAARGTALRRLVPWILFLIFFTVMN
                     ETVFNVSTPKIAEQFGLGPAGVSWVMTVFLVFFGIGSVIYGRLSDLFSLRFLILIGVI
                     VYCVGSVAGFLLQSSYPLVLAARGIQGAG"
     gene            434..1726
                     /locus_tag="VFH83_12030"
     CDS             434..1726
                     /locus_tag="VFH83_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487144.1"
                     /translation="MNENAVRSAIQEAIRSTPVVDPHCHLDPGHPGSRSFDDILLYHH
                     VWIELVSCGLPQKEVTAAGLPQELVDPGMDPLERIRRARPYFHAIRNTVSSLFLRWLL
                     RDLFEWEEDLEVSDPRKLQERMDGMLSRGGWAEKVFQRCRIERCISVEKGGNEGTPRI
                     LKGAETLGIVNLVDGKRSARETLASKDAELGREIRTAGDYREYLVRKLDVVPPESLRF
                     LGAWVAPSITDELATDEEVTRILGRARDGRALSQEETGSFCYFGLCAGLDHLKKSRLR
                     TIQVIVGAEVLPPHRSIPQWDGRFTAALARIAGAYEEFRFNVSTASEHYLQDIAILAK
                     HLPNISVAGYWWHTLYPNYIRLALETRLDIVPANKIVAFFSDAYHCEWIYPKLRLVES
                     ILEDILVQRVMQDRYSLPTATSLVPTLLHDAPTDIYLR"
     gene            complement(1808..2863)
                     /locus_tag="VFH83_12035"
     CDS             complement(1808..2863)
                     /locus_tag="VFH83_12035"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6487145.1"
                     /translation="MAKLKIGVVGCGIMGVRHLAVAAASPLAEAFAVADTLPDRARAA
                     AEKYGAKRVFSDGRELIADPDVQAVVFSTPTGRRDELVIAALERGKHVLAEKPIAMNA
                     ETVRRMIRARGDRVAACCSSRFHAFESARVAARFVSTGALGDIRVVHCRVHDPAGPPP
                     KSPPPTWRLSRRENGGGILVNWGCYDLDYLLGVCNWSLRPRTVFAQTWQVPPRLRANV
                     APDSDAETHVAALILCDGGAVISYERGEYMPAHGQQAWQVIGTHGSLNLNMLYQEGKV
                     MTHDEVTAEAGLVTREIWRGTETWEQMQDYVLTDFIQAVVEGRPPHTTLEKALVLQQI
                     TDAVYASAATGMAVPIP"
BASE COUNT          547 a          967 c          945 g          465 t
ORIGIN      
        1 ccggctccct ggatgccgcg cgccgccaga accagggggt acgaggactg gagcagaaac
       61 ccagcgacgg aaccgacgca atatacgatg acgccgatga ggatcaggaa ccggaggctg
      121 aagaggtccg agagccgtcc gtagatcacc gagccgattc cgaagaatac caggaacacc
      181 gtcatgaccc agctcacccc cgccgggccg agaccgaact gctccgcgat cttcggggtg
      241 gagacgttga agaccgtctc gttcatgacg gtgaagaaga tcagaaagag gatccatgga
      301 acgagccgcc gcagagccgt ccctcgtgcg gcgacagagg agccatccga ggcgggcgcc
      361 tccggagacg agtcactggg tatgccacgc atggtccgct caatataatc agccgcggcc
      421 cgggaggata tcgatgaacg agaacgccgt acgctcagcc attcaggaag ccatccggag
      481 cacgccggtg gtggatcctc actgccacct cgatcccggg catcccggct cccgcagctt
      541 cgacgatatc ctcctgtacc accacgtctg gatcgagctt gtgtcctgcg ggctgccgca
      601 gaaggaggtg acggcagcgg ggttgcccca ggagctcgtg gatccgggga tggatcccct
      661 ggagcggatc cggcgggcca gaccctactt ccacgcgatc cgcaatacgg tgagcagcct
      721 cttccttcga tggctcctgc gcgatctgtt cgagtgggag gaggacctcg aggtctcgga
      781 cccgcgcaag ctccaggagc gcatggacgg gatgctgtcc cgagggggat gggctgagaa
      841 agtcttccag cggtgccgga tcgaacggtg catcagcgtc gagaaaggcg ggaacgaagg
      901 gaccccgcga atcctgaaag gcgccgagac gctcggaatc gtgaacctcg tcgacggaaa
      961 gcgttcggct cgggagacgc tggcctcaaa ggacgcggag ctcggaaggg agatccgaac
     1021 cgcgggagac tatcgcgagt acctggtgag gaagctggat gtcgttccac cggaatccct
     1081 gcgcttcctg ggcgcctggg tggcgccctc catcacggat gagctggcga cggatgagga
     1141 ggtcacccgg atcctgggac gcgccagaga cgggcgagcc ctgagccagg aggagacggg
     1201 cagcttctgc tacttcgggc tgtgcgcggg cctggaccac ctcaagaagt cgcggctgcg
     1261 aacgatccag gtgatcgtcg gcgcggaggt gcttcctccc catcgctcca tccctcagtg
     1321 ggatggtagg ttcaccgccg cgctggcacg gatcgccgga gcctacgagg agttccggtt
     1381 caacgtcagc acggcttccg agcactacct ccaggacatc gccatcctgg ccaagcacct
     1441 ccccaacatc agcgtggcgg ggtactggtg gcacacgctc tatccgaact acatccgcct
     1501 ggcgctggaa acccgattgg atatcgtgcc ggcgaacaag atcgtggcct tcttctccga
     1561 tgcctaccac tgcgaatgga tctaccccaa gctccgactg gtggagtcga tcctggaaga
     1621 catcctggtg caacgggtga tgcaggatcg ctattcgcta ccgacggcaa ccagcctggt
     1681 ccccacgctg ctccacgacg ccccgacgga catctacctg cgatagaccc cgtgaccgct
     1741 ccgctcggga tgcggtgcct cagtccggcg gtgcctcagc cccgtgggat ttccgtccct
     1801 cgccgcctca gggaatggga acggccatgc cggtggctgc ggaggcgtag acagcgtcgg
     1861 tgatctgctg gagcacgagc gccttctcca gtgtggtatg aggagggcgg ccctccacca
     1921 cggcctggat gaagtccgtg agcacgtagt cctgcatctg ctcccaggtc tcggtcccgc
     1981 gccagatctc gcgggtcacc aggccggcct cggcggtgac ctcgtcgtgg gtcatcacct
     2041 tcccttcctg gtagagcatg ttcaggttca gcgacccatg ggtcccgatg acctgccacg
     2101 cctgctgccc gtgggccggc atgtactccc ctcgctcata gctgatcacg gccccgccgt
     2161 cacagaggat cagcgcggcc acgtgcgtct cggcgtcgga gtccggagcc acattggcgc
     2221 gcaggcgcgg cggcacctgc caggtctggg cgaacacggt ccgaggtctc agcgaccagt
     2281 tgcatacgcc caggaggtaa tccaggtcgt aacagcccca gttcaccaag atgccgccgc
     2341 cgttctctcg gcgggagagc ctccaggtcg gaggagggct cttgggagga ggcccggcgg
     2401 gatcgtgcac ccggcagtgc accacccgga tgtcccctaa agcgccggtg gaaacgaacc
     2461 gcgctgccac ccgggcggac tcgaacgcgt ggaaacgcga cgaacaacat gcggccaccc
     2521 ggtccccgcg agcgcggatc atgcggcgga cggtctcggc gttcatggcg atcggcttct
     2581 ccgccagaac gtgtttgcct cgctccaggg ccgcgatgac aagctcgtct ctacgccccg
     2641 tgggagtgct gaacaccacc gcctgcacat ccgggtccgc tatcagctca cggccatccg
     2701 agaacacgcg cttcgcccca tacttctcgg ccgccgccct ggcccggtcc ggcagggtgt
     2761 ccgccacggc gaaggcctcg gcgagcggag aggccgccgc cacggcaaga tggcgaactc
     2821 ccatgatgcc gcacccgacc acaccgatct tgagcttcgc catgtcagag ccccagatcc
     2881 ttcaggacgc ttcggatgta gatcagatcc tgcggggcga acca
//
LOCUS       DASRZS010000493         6734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4499568, whole genome shotgun sequence.
ACCESSION   DASRZS010000493 DASRZS010000000
VERSION     DASRZS010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4499568"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFH83_12040"
     CDS             complement(<1..216)
                     /locus_tag="VFH83_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET6487146.1"
                     /translation="MDAEITLKTSVDVAHIRRACRVAERCLRSLAPCVRPGISTGELD
                     RLAVRFLAENHAASALRGYRGFPGTICA"
     gene            complement(201..1760)
                     /locus_tag="VFH83_12045"
     CDS             complement(201..1760)
                     /locus_tag="VFH83_12045"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HET6487147.1"
                     /translation="MTDIPSVWHVTREYAGLAEAGGIKDVVRGLAEASVRAGIETSVV
                     LPRYGFLPDTLTRGTPVAEFHVSLPDHDRGDQFFDEPVRMFSHVEQGVRIFLVDSPRY
                     ASKRNVYTYTEADHASNEWQKPGTGHWDSHQLNLILQRATLETLLAIDEHLAPMPRII
                     HCHDGHAAFLPAIARELVRYRGRLADTGLVLTIHNAGKGYHQEVWDRGFARLLTGLDP
                     TVLAKGSIGRTVDPLLLAGSYASVVTVSEQYARELLAERADELSGGLGRAFRERGIDL
                     RGVTNGLDPSPWDPRGLAAGLPQRFDPSTGDLAGKRACREALASRVGLSRRSTPTPLF
                     AFVGRLTGQKGIDILLEALGSLLSEGAMLQCVVLGQGEAADEQRMAGLARAHAGTLSF
                     VPRYDAELSRLIYAASDFFLVPSAYEPCGLTDFIAQIAGSVPIVHRVGGLVKVRDGET
                     GLSYDEQSAAALGGQIRRACGLFQDHPEVLERIRVTAFNEIFRLHTWDRVLATGYLPE
                     YRTAVERWTLR"
     gene            complement(1757..2119)
                     /gene="aroH"
                     /locus_tag="VFH83_12050"
     CDS             complement(1757..2119)
                     /gene="aroH"
                     /locus_tag="VFH83_12050"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET6487148.1"
                     /translation="MTQAVRGAIQVAQNSRPSIHRAAARLVREMLDRNAVDENDIVSI
                     LFSVTGDLSAANPATGLREVGFAETPLFCCQEPPVDGGMPRVIRVLLTWECARRTRGV
                     PVYLDGAATLRPDLEPAS"
     gene            complement(2119..3273)
                     /locus_tag="VFH83_12055"
     CDS             complement(2119..3273)
                     /locus_tag="VFH83_12055"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET6487149.1"
                     /translation="MDLGVTSFQLEIPREIRFGAGALDGLRGIVEPIGTRVLLVSGDR
                     WLGDSPWGSRILAALDGLTVHPVRCAKGEPTSESIANALAAAEETRPQAIVAVGGGSV
                     IDTAKALSGLLAHGGPVERFLEGNVGSVPVPGPGVPWIAIPTTAGTGAEVTKNAVVRS
                     QSQGVKRSMRSAHLLARAVLVDPLLTVSLPPSVTGTAGLDALTQLVEAYVSRGSNPFV
                     RSIVEGAFPGMVTALRGLAASPSDPELRALASYGALMSGIALANAGLGAAHGFAGALG
                     GMFDIPHGLACAVLLSPVLRANRDVIRPYLERLTAPCGREPGSDPVEWMVATVRDLLT
                     AYGLPDDLRAFKVGAEAVEEIVRKSSGSSMRGNPRELSPAERAGILSEVL"
     gene            complement(3283..3618)
                     /locus_tag="VFH83_12060"
     CDS             complement(3283..3618)
                     /locus_tag="VFH83_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487150.1"
                     /translation="MRAYPFPRRLSSFAGRAVAFFFVLSILLLYIYLVGNYQDFLDSS
                     QIYLLSLLRISLVLELASGFALAVFLGWRVARDHRPFVGRWLLLLGSMVCAFGLLAAL
                     RFAQQWFQS"
     gene            3712..4221
                     /locus_tag="VFH83_12065"
     CDS             3712..4221
                     /locus_tag="VFH83_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET6487151.1"
                     /translation="MPEGGYFFNFDKLAYVRGRHPAGCILCLARDGSPDVVDLSIWRD
                     DLFVASVNLYPYNPGHLLVYPQRHVEDIRELTAEEERRMAGIQRWLLDLLDRACAPHA
                     YNIGFNMGAAAGASINHLHLHIIPRYPKETGIADLIAGRRVLVEDPRETARQLREMAA
                     REPYSSRPT"
     gene            complement(4200..5966)
                     /locus_tag="VFH83_12070"
     CDS             complement(4200..5966)
                     /locus_tag="VFH83_12070"
                     /inference="COORDINATES: protein motif:HMM:NF036553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HET6487152.1"
                     /translation="MPVRSRLEELSLALPETERKQLLERIARRAEREQDEEAIVVELR
                     DDERERVITAEIAKAGLWVRFVLWLRMLLTTRSREEVFLDLKLGTLKSRIRATAPGVT
                     GFDSRDLTARVAHLLHQLYLRVLPLMPIYHTLGSDKNVRGAAYSWLIDRRWEGAHREV
                     LDFVPLQEMEELISQTDNVEEVTKKLSVRLTEYIRSVPDSLVADAEAQAALHLTLTRL
                     AAFPFATFFGYFGYTLADDDAVPPVFVPAPAMLALDLMERLYACLSLLAEAGPSYSFA
                     EEPVAYVLLLRAGLIPAEEPDLEKIKPALDDLRSKIQDLAKQAAAFQRAVPLLDLVRW
                     FRRDPWYQIVANPPRLFLKSLYFTTLKARLGAELARHLTKIQEQVVSRRISEVLKGKK
                     QVELPHCRIPEDVDLKSLHLPGFSTTRSLTFLYNYLVQQFRGAFQEAAQLLGSTLLAN
                     NRLLQNRISLSIAGLEDMEARIVLYDRSLSSDEEDGRILERYLATLANDPVTQKAFRS
                     LVVQKDREARELLEKAKEYLATLRGIFDDLIERATDSSRSFLKTLHLFRGKQQTLGQV
                     LKGRSEAIGDFLKVLDQVGRLE"
     gene            complement(5968..6594)
                     /locus_tag="VFH83_12075"
     CDS             complement(5968..6594)
                     /locus_tag="VFH83_12075"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET6487153.1"
                     /translation="MTGLLFVGGEGPGREVLSRCAREAALSVAADSGLELALSAGIEP
                     DLVVGDMDSLTDLTLLQRFPPDRVVRLPRDKDDTDTEVGLQTLLDRGADEVTIVGGGG
                     GRMDHLLGIVSLFQRGRPPRRWLTHRDEIILVDRDASFEGWIGSTVSVFPVGERAARM
                     RSTGLRWPLDGLVFEKGRVGISNIVTQDPFLLSVADGKLLVIRSRLEG"
BASE COUNT         1196 a         2305 c         2233 g         1000 t
ORIGIN      
        1 ggcgcagatg gtaccgggga agccgcggta tccgcggagc gccgaggcgg catggttttc
       61 cgccaggaac cgcacggcga ggcggtccag ctccccggtg ctgatgccag gccgcacgca
      121 gggcgcgagg ctccgcaggc atcgctcggc cacccggcac gccctgcgga tgtgggcaac
      181 gtccaccgag gtcttgaggg ttatctcagc gtccatcgct cgaccgcggt acggtactcc
      241 ggcaggtacc ctgtggccag cacgcggtcc caggtgtgca ggcggaagat ctcgttgaac
      301 gccgtcacgc ggatccgttc gaggacctcg ggatggtcct ggaagaggcc gcaggcgcgg
      361 cggatctggc ctcccagagc ggccgcgctc tgctcgtcat aggagagccc ggtttccccg
      421 tcgcggacct tcaccaggcc tccgacccga tgcacgatag gaacgctccc ggcgatctgg
      481 gcgatgaagt ccgtcaaccc gcagggctcg tacgccgagg ggaccaggaa gaaatcgctt
      541 gccgcataga tgagccggga cagctccgcg tcgtaccgcg gcacgaagct gagcgtgccc
      601 gcgtgggcac gggccagccc cgccatccgc tgctcatcgg ccgcctcccc ctgtcccagg
      661 acgacgcact ggagcatggc cccctccgac agaaggctcc ccagcgcctc caggaggatg
      721 tcaatcccct tctgtccggt gagccggccc acgaacgcga aaagcggcgt gggagtcgac
      781 cgtcgggaca aaccgaccct ggaggccagt gcttcccggc acgctcgctt gccggccagg
      841 tcaccggtcg acgggtcgaa gcgctgaggc agaccggccg ccagcccgcg gggatcccac
      901 ggagacgggt ccagcccgtt ggttacgccg cgcaggtcga tgccgcgctc gcggaaggcc
      961 ctgcctagcc cgccgctcag ctcgtccgcc cgctccgcca gcaactctcg ggcgtactgc
     1021 tcggagacgg tgaccacgga agcgtaggag ccggcgagca gcagcggatc cacggtccgg
     1081 ccgatcgatc cctttgccag aaccgttgga tcgagaccgg tgagcaaccg ggcgaaccca
     1141 cgatcccaga cctcctggtg ataccccttt cccgcgttgt gaatcgtgag cacaaggccg
     1201 gtatcggcaa gccgcccccg gtatctgaca agctcccgcg caatggcagg aagaaacgcg
     1261 gcgtgcccgt cgtggcagtg gatgatcctc ggcatggggg cgaggtgctc gtcgatcgcc
     1321 agaagggttt ccagggtggc gcgctggagg atcaggttga gctggtgcga atcccagtgg
     1381 ccggtcccgg gcttctgcca ttcgttgctc gcgtggtccg cctcggtata ggtgtagaca
     1441 tttcgcttgg aggcgtagcg aggcgaatcc acgaggaaaa tccgcacgcc ctgctcgaca
     1501 tgggagaaca tgcggaccgg ctcgtcgaag aactggtctc cccggtcatg gtccggcagc
     1561 gagacatgaa actcggcgac gggcgttccg cgcgtcagcg tatccggtag aaagccgtac
     1621 cgggggagaa ccacggaggt ctcgatcccc gctcggaccg atgcctcggc cagccctcga
     1681 accacgtcct tgatcccgcc ggcctccgcc aggccggcgt actcccgcgt gacgtgccag
     1741 acggacggga tgtccgtcac gaggcaggct ccaggtccgg cctgagggtg gcggccccgt
     1801 ccaggtacac gggcacgcca cgggtcctcc gagcgcactc ccaggtgagg aggacgcgga
     1861 tgacccgagg cattccgcca tccaccggcg gctcctggca gcagaagagc ggtgtctccg
     1921 cgaagccaac ctcccgaaga cccgttgccg ggtttgccgc cgagaggtcc cccgtgacgg
     1981 agaagagtat gctcacgatg tcgttctcat cgaccgcgtt ccgatccagc atttcgcgga
     2041 ccagccgggc ggcggcccgg tggatcgaag gccgtgagtt ctgcgctacc tggatagcgc
     2101 ctcggactgc ctgggtcatc agaggacctc ggaaaggatg ccggcgcgct cggcggggga
     2161 caactcccgg gggtttcccc gcatgctgct gccggaagac ttccggacga tctcctccac
     2221 ggcctcggcg ccgaccttga acgcgcgaag atcgtcgggc aggccgtagg cagtcagcag
     2281 gtcccgcacc gttgccacca tccattccac ggggtcagag cccggttctc ttccgcaagg
     2341 cgccgtcaag cgctccagat agggacggat gacgtcccga ttggccctca ggaccggtga
     2401 aaggaggacc gcgcaggcaa gaccgtgcgg gatgtcgaac atccctccca gagccccggc
     2461 gaacccgtgc gcggcgccga ggccggcgtt ggcgagcgcg atgccgctca tcagcgcccc
     2521 gtagctggca agcgctcgca gctcgggatc cgacggactc gcagccagcc cacgaagcgc
     2581 ggtcaccatg ccggggaacg cgccctccac gatcgaccgg acaaaaggat tcgagcctcg
     2641 gctgacgtaa gcctccacga gctgcgtgag cgcatccagc cccgcggttc cagtaacgga
     2701 agggggaagc gaaacggtca ggagcgggtc caccaggacc gcccgcgcca ggagatgggc
     2761 ggaacgcatg ctcctcttga cgccctggct ctgggacctc accacggcgt tcttggttac
     2821 ctccgcgccc gtccccgccg tggtcgggat cgcgatccag ggcacgccag gaccggggac
     2881 gggcaccgaa ccgacgttcc cttccaggaa acgctccact ggcccaccgt gagccaggag
     2941 accggataga gccttggccg tgtcgatcac ggagccgccg ccgaccgcaa cgatcgcctg
     3001 cggtcgcgtt tcctcggcgg ctgccagcgc gttcgcgatg ctctcactgg taggctctcc
     3061 cttggcgcat cgcaccggat ggaccgtcag gccatcaagg gctgccagga tccgcgatcc
     3121 ccacggggag tccccgagcc agcgatcgcc agagaccagg agcacccgcg tcccgatcgg
     3181 ctcgacgatg cctcggaggc cgtcgagcgc gccggccccg aagcggatct ctcgaggaat
     3241 ctcgagctgg aacgacgtca cgcccaggtc catctccgcc tctcaggact ggaaccactg
     3301 ctgggcgaaa cgcagggcag ccagcaggcc gaacgcgcac accatcgagc cgagcaggag
     3361 cagccaccgc ccgacaaagg gccggtggtc cctggccacg cgccagccca ggaacaccgc
     3421 cagcgcgaaa ccggacgcca gctccaggac cagggagatc cgaagcaggg agagcaggta
     3481 gatctggctg gaatccagga aatcctgata gttgccaacg aggtagatgt agaggagcag
     3541 gatcgacagc acgaagaaga aagcgaccgc gcgtcctgca aaactggaca gtctacgcgg
     3601 aaatggatat gctctcatgc accttcgatt atagtctggc ggaggcggcg ccgggaaggg
     3661 tcatcctgga gtcccgcgcc ccgaccgacc aggacgccga cggagttacg cgtgcccgag
     3721 gggggctact tcttcaactt cgacaagctg gcctacgttc gcggccgcca cccggcgggc
     3781 tgcatcctgt gccttgctcg agacggaagc ccggatgtag tggacctcag catctggcgg
     3841 gacgacctgt ttgtcgcctc cgtgaatctc tacccctaca acccgggtca cctgttggtc
     3901 tacccgcagc gccacgtcga ggacatccgc gagctgaccg cggaggagga gcggcggatg
     3961 gcgggcatcc agcgatggct ccttgacctg ctggaccgcg cctgcgctcc gcacgcctac
     4021 aacatcgggt tcaacatggg ggccgcggcc ggagcctcca tcaaccacct gcacctgcac
     4081 atcatcccga ggtatcccaa ggagaccggg atcgccgatc tgatcgccgg gaggcgcgtc
     4141 ctggtggaag acccgcggga gaccgcgcgt cagcttcggg agatggccgc ccgcgagccc
     4201 tactccagcc ggcccacctg atccaggacc ttcaagaagt cgccgatggc ctccgatcgt
     4261 cccttgagga cttgacccag ggtctgctgt ttgcctcgaa acaagtgcag cgtcttgagg
     4321 aacgagcgcg agctgtccgt ggcccgctcg atcagatcat cgaagatacc ccgaagggtg
     4381 gccaggtatt ccttggcctt ctccaggagc tcccgcgcct cccggtcttt ctggaccacc
     4441 agggagcgga acgccttctg ggtgaccggg tcattggcca gggtcgccag gtaccgctcc
     4501 aggatccgac catcctcctc gtcgctggac aacgagcggt cgtacaggac gatccgcgcc
     4561 tccatgtctt ccagcccggc gatgctcagc gagatccggt tctgcaggag ccggttgttg
     4621 gcaaggaggg tgctccccag gagttgggcc gcctcctgga aggctccccg aaactgctgc
     4681 accaggtagt tgtacaggaa ggtgaggctt cgggtggtgg agaaccccgg caggtgcagg
     4741 gacttcagat cgacgtcctc ggggatacgg cagtggggga gctccacctg cttcttcccc
     4801 ttcagcacct cggagatcct tcggcttacc acctgctcct ggatcttggt gaggtgccgg
     4861 gccagctcgg cgccgagacg ggccttcagc gtcgtgaagt agagactctt gaggaagagg
     4921 cgcggaggat tggccacgat ctggtaccac gggtcccgac ggaaccatcg caccaggtcc
     4981 agcagcggca ccgcccgctg gaacgcggcg gcctgtttgg cgaggtcctg gatcttggag
     5041 cgcaggtcgt ccagggccgg cttgatcttc tcgagatccg gctcctccgc tggaatcaga
     5101 cccgcccgga gaagcaggac ataagcgacc ggctcttcgg cgaacgaata ggaggggccc
     5161 gcctcggcca gcaagctcag acaggcgtac agccgctcca tgaggtccag ggccagcatg
     5221 gccggcgcgg gcacgaagac gggagggact gcgtcgtcgt ccgccagggt ataaccgaag
     5281 tacccgaaga acgtggcaaa cggaaacgcg gccagtcgag tgagggtgag gtggagcgcc
     5341 gcctgcgcct cggcgtccgc gaccaggctg tccggaaccg aacggatgta ctcggtgaga
     5401 cggacggata gcttcttggt gacctcctcg acgttgtccg tctgggagat gagctcctcc
     5461 atttcctgga gagggacgaa gtccaggacc tcccgatggg cgccctccca gcgccgatcg
     5521 atcagccagc tataggcggc tccccgcacg ttcttgtccg agcccagcgt gtgatagatc
     5581 ggcatgaggg ggagcacgcg caggtaaagc tgatggagga ggtgcgccac ccgggcggtc
     5641 agatcccgcg aatcgaatcc cgtgaccccc ggagccgtgg cccggatccg gctcttgagg
     5701 gtgccgagct tcaggtcgag gaagacctcc tcacggctac gcgtggtgag cagcatccga
     5761 agccacagca cgaagcggac ccagaggccg gccttggcga tctcggccgt gatcacccgc
     5821 tcacgctcgt cgtcgcgcag ctcgacgacg atggcctctt cgtcctgctc gcgctccgcc
     5881 cgccgcgcga tacgctccag gagctgcttg cgctccgtct cgggaagcgc caacgagagc
     5941 tcctccaggc gactccttac cggcatgcta tccctcgagg cgcgatctta tcacgagaag
     6001 cttgccatcg gccaccgaga ggaggaaggg gtcctgggtg acgatattgc tgatcccgac
     6061 ccgtcccttc tcgaacacga ggccgtccag gggccaccga aggccggtgg agcgcatccg
     6121 cgccgctcgc tctccgacag ggaacaccga cacggtgctg ccgatccaac cctcgaagct
     6181 ggcgtcccgg tccacgagga tgatctcgtc gcgatgcgtg agccagcggc gaggcggccg
     6241 cccccgctga aagagcgaga cgatgcccag gaggtggtcc atccgcccgc ctccaccgcc
     6301 cacgatggtc acctcgtccg ccccgcggtc cagaagggtc tggaggccca cctcggtgtc
     6361 cgtgtcgtct ttgtcccggg gcaggcgcac tacgcggtcc ggcggaaagc gctggagcag
     6421 cgtcaggtcc gtcagggaat ccatgtcgcc gaccacgaga tccggctcga tgccggcgga
     6481 aagggcgagc tccagacccg agtccgcggc aacgctcagg gcggcctccc gggcgcagcg
     6541 cgacagcacc tcgcgccccg ggccctcccc gccgacaaac aacaaaccgg tcaccgctcg
     6601 cctcccctcc ctcgtcgaga gtagcgcgga tcgcggcggg cggcaatcgc ctccgcgacg
     6661 ggcgcgcgtc ccgcagacgc gacgggcgcg cgtcccgcag acgcgacggg cgcgcgtccc
     6721 gcagacgcga cccg
//
LOCUS       DASRZS010000494         2825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_270476, whole genome shotgun sequence.
ACCESSION   DASRZS010000494 DASRZS010000000
VERSION     DASRZS010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270476"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFH83_12080"
     CDS             complement(<1..255)
                     /locus_tag="VFH83_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487154.1"
                     /translation="MSRRWILVVVLAVGLVGSLWSGGKAEGGAAGKSVQLTQWVFPFA
                     TEQEDRAMFGAILTEFQKTNPNISVNLEFFPWDARRERMST"
     gene            complement(303..1145)
                     /locus_tag="VFH83_12085"
     CDS             complement(303..1145)
                     /locus_tag="VFH83_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6487155.1"
                     /translation="MRYRRRRPDEWRDVVVISVILIAFGVAALFPLLWMVSTSLKPSG
                     SIYQYPPQFIPKQPSLAAYGTLFRQPDFPNWFKNSVIVTSLTVVLNLLVSAAAGYAFA
                     KTRFPGRQFLFVLILATLMIPSFSVLIPLFTMIVKWSMVNTYWALVLPQVASPVGIFL
                     LKQYTETLPDELINAAWIDGAGTFRVFFTIIIPLVTPALAAAAIFAFLNSWNSFFWPL
                     VATTATRMRTLPVGLAVFQGQYRSEWALICAGSFMTLVPALALYIALQRYFVQGIAMT
                     GLKG"
     gene            1297..>2825
                     /locus_tag="VFH83_12090"
     CDS             1297..>2825
                     /locus_tag="VFH83_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N'-diacetylchitobiose phosphorylase"
                     /protein_id="HET6487156.1"
                     /translation="MRYGHFDNEQREYVIDRVDVPVSWTNYIGVNTMCAVLSHNAGGY
                     SFYRHAEHGRITRFRPNGVPLDRPGHYVYVRDDSDGDYWSISWQPVGKPLANARYECR
                     HGLSYSRYRAEYRDIAAEQLVFITRQDDVELWDVRVTNTGSTPRDLSLFSYVEFSFHH
                     IEIDNQNLQMSLYAAGSSYTDGIIEYDFFYEPWTFHYHTASVPLDGFDTMRDAFLGPY
                     RTETNPLAVERGSCSSSAEVTGNHCGALHTRLHLEPGEQRRVTFMLGYGDKAAGAAAR
                     SRYSVPSSVDAAFADLRSYWEAKCAVFQVRTPHEGLDTMVNTWTLYQAETCVVWSRFA
                     SFIEVGGRTGLGYRDTSQDIMACVATNPAKVRQRIRELLNGQVSAGYGLHLFDPDWFD
                     PEKQKRPKFRSPTIAHAPDKTSYIHGIEHACSDDHLWLIPSICEFVKETGDLAFFDEV
                     VPFCDAGQATVWEHMKRALDFSAAQVGPSGICLGLRADWNDCLNLGGGESGMVSFLHH
                     WA"
BASE COUNT          528 a          918 c          900 g          479 t
ORIGIN      
        1 cgtggacatg cgctcccggc gggcgtccca cggaaagaac tccaggttga cgctgatgtt
       61 cgggttcgtc ttctggaact cggtcaggat ggcgccgaac atggcccggt cctcctgctc
      121 ggtggcaaag gggaacaccc actgcgtgag ctgcacgctc ttccccgctg cgccgccttc
      181 cgccttgcct ccggaccaga ggctgcccac cagccccacc gccagcacca cgacgaggat
      241 ccatcgtctg ctcatcgtcg tcctcctcct gcgaatgtgt gtcatcgccc aacgggcgct
      301 tctcacccct tcaacccggt catggcgatg ccctggacga agtaccgctg cagcgcgatg
      361 tacagggcca gcgcgggcac cagcgtcatg aacgagcccg cgcagatcaa ggcccactcg
      421 gaccggtact ggccctggaa gacggccagg cccacgggaa gcgtccgcat ccgcgtggcg
      481 gtcgtggcca ccaggggcca gaagaacgag ttccacgagt tgagaaacgc gaagatcgcc
      541 gccgccgcca gggccggagt caccagcggg atgatgatcg tgaagaagac gcgaaaggtt
      601 ccggccccgt cgatccaggc cgcgttgatc agctcgtccg gcagggtctc cgtgtactgc
      661 ttgagaagaa agatgccgac ggggctcgcc acctggggca gcaccagggc ccagtaggtg
      721 ttcaccatgc tccacttcac gatcatcgtg aacagcggaa tgagcacgct gaaggacggg
      781 atcatcagcg ttgccaggat caggacgaaa aggaactgcc ggcccgggaa gcgggtcttg
      841 gcgaaggcgt agccggcggc ggcggagacc agaaggttca gcaccaccgt gaggctcgtg
      901 acgatcacgc tgttcttgaa ccagttcggg aagtccggct ggcggaacag ggtcccatag
      961 gcggcgagcg acggctgctt ggggatgaac tgcggcgggt actggtagat cgagccggac
     1021 ggtttgaggg acgtggagac catccagagc agagggaaca gggccgcaac gccgaacgcg
     1081 atgaggatga cggagatgac caccacgtcc cgccactcgt cgggcctacg tcttcgatac
     1141 ctcatgctgg gacctgatcc cttcgtgacc atatacgggc cggctccgcg ctgtcaacac
     1201 ggaatccggt catgccgcgg cgcggccttt cgttgaccct cgtccatgcc gagcggtaga
     1261 ctggcttcac cgacgtccgc gaccacggag ggcttcatgc gctacgggca cttcgacaac
     1321 gagcagaggg agtacgtcat cgaccgggtg gacgttcccg tttcgtggac caactacatc
     1381 ggcgtgaaca cgatgtgcgc cgttctttcc cacaacgcgg gtggctactc gttctaccga
     1441 cacgcggagc acggaaggat cacccgcttt cggccgaacg gggtgccgct ggaccggccg
     1501 ggacactatg tctatgtccg agacgattct gacggggact actggagcat ctcgtggcaa
     1561 cccgtgggta agcccctggc caacgcgcgc tacgaatgcc ggcacggtct gtcctattcg
     1621 cgctaccggg cggaataccg cgacatcgcc gcggagcagc tcgtcttcat aacccggcag
     1681 gacgatgtcg agctttggga tgtgcgggtg acaaacaccg gctccactcc gcgagacctg
     1741 agtctcttct cgtacgtcga gttctccttt caccacatcg agatcgacaa ccagaacctg
     1801 cagatgagcc tgtacgccgc gggctccagc tacacggacg ggatcatcga gtacgacttc
     1861 ttctacgagc cgtggacgtt ccactaccac accgccagcg tgccgctcga tgggttcgac
     1921 accatgcggg atgcctttct gggtccctac cgcacggaga cgaacccgct ggccgtggag
     1981 cggggaagct gctcgtcgag cgcggaggtc acgggcaacc actgcggcgc cctgcacacc
     2041 cgcctccacc tggagccggg cgagcagagg cgggtcacct tcatgctcgg ctatggagac
     2101 aaggccgcgg gcgccgccgc tcggtctcgg tattcggtgc cgtcgtccgt tgacgccgct
     2161 tttgcggacc tcaggtccta ctgggaggcg aaatgcgccg tcttccaggt ccgcacgccc
     2221 cacgagggcc tcgacaccat ggtaaacacc tggactctgt accaggcgga gacctgcgtt
     2281 gtctggtccc ggtttgcttc cttcatcgag gtcggaggca gaacggggtt gggataccgg
     2341 gataccagcc aggacatcat ggcctgcgtc gccaccaacc cggccaaggt ccgccagcgc
     2401 atccgcgagc ttctcaacgg gcaggtgagc gccgggtatg ggctgcacct cttcgacccg
     2461 gactggttcg atcccgagaa gcagaagcgg ccgaagttcc gaagccccac catcgcccat
     2521 gcgcccgaca agacgagcta catccatggg atcgagcacg cctgctccga cgatcacctg
     2581 tggctgatac cgtcgatctg tgagttcgtg aaagagaccg gcgacctggc cttcttcgac
     2641 gaggtcgttc cgttctgcga cgccgggcag gctacggtct gggagcatat gaagcgcgcg
     2701 ctggactttt ccgcggcaca ggtgggcccg agcggcatct gcctgggcct ccgtgcagac
     2761 tggaacgact gcctcaacct cggcggcggc gaaagcggca tggtgtcctt ccttcaccac
     2821 tgggc
//
LOCUS       DASRZS010000495         6331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4122117, whole genome shotgun sequence.
ACCESSION   DASRZS010000495 DASRZS010000000
VERSION     DASRZS010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4122117"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1409)
                     /locus_tag="VFH83_12095"
     CDS             complement(39..1409)
                     /locus_tag="VFH83_12095"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HET6487157.1"
                     /translation="MPDSVNEVRPFRALHYDPTKVKDIGLCLSQPYDVISAEQAEAYY
                     AQHPYNVIRLILNRVEPGDNDGNNRYTRARDLLSAWRADGALRRTARPSFWVYEQEFD
                     LPGIGRKRVKGFIGAVRLREYAERRILPHEKVMDGPLGDRIRLTETANTQFEYIWSLY
                     QDKAYVIDNILDDVERETPILDYREQPIGVRHRLWRLTDPERCGIISRTMARLRIYIA
                     DGHHRYQTMLTVRERMRRQYPDAGPDAPWEHIMMFLVNSEHEGLTILPTHRMLHGIQV
                     ESIYKLGADILEHFHVKKYVFRDADESEIRRRWLRDLRDVSPGEHKFGAYIVNTTGYY
                     LLTLRDEEAYEELVRLDTSSEWKLLDVNILNTLILDGILGITEENLNHSTSIAYTKDA
                     DEAIARVRRGEIQVALILNPTALGDVITIAENDERMPRKSTHFYPKPVSGLVFYPMDP
                     AFHREG"
     gene            1575..2165
                     /locus_tag="VFH83_12100"
     CDS             1575..2165
                     /locus_tag="VFH83_12100"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HET6487158.1"
                     /translation="MRVAAFLGSPRPGGNTDTLADAILDGAREAGLETDRHVLRSLRV
                     HPCTGCELCWTKGRPCIFQDDGTALYDSVASSDVLLFATPVYWYGATAILKAFIDRLV
                     VFNRPQGRPLVQGKRAVLVSAWEEKGMEAVEPLVRQFELSFRFLELRFTDRLLVDELG
                     PRDAVRQRPALVARARALGKALVRVETCIDEKTRAT"
     gene            2143..3918
                     /locus_tag="VFH83_12105"
     CDS             2143..3918
                     /locus_tag="VFH83_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487159.1"
                     /translation="MKKPEQPEGLKIPFRGYWDILSRYLTKRSGTFVLLAVLICTGIG
                     LQLANPQLLRGVIDGALAGRPSSTLVLSAVAFILVALTQQLLGVAAAYVGENLAWKAT
                     NELRSDLAEHCVSLDMRYHNDTTPGELIQRIDGDVAEFSNFFSQLVVRILANVLLMIG
                     ILGVLYFENPAIGGVFTFFAALTIGTLSFVKNLAVGPEKLLREANTDLSGFLEERLAG
                     TEDIRSSGAVDYVLNGLYSLHRKILTFWRQAGLMHFWVRMISGVVLTIGYAAAFVGGY
                     TLYRSGAMTVGTVFLLIYYMGLLARPVRELAQQVDSLQSVGASVERIRELKALRPTIV
                     DGQGPDLPSHQPLRLEFESVGFAYLEGEQVLDSLSFRLPPGHILGLLGRTGSGKTTIA
                     RLVFRLYEPTMGRVLLDGIPLPDTRLSDLRARVALVTQDVQLFQATVRENITFFDRSI
                     PDSAILRAIESLELDQWLAGLPLGLDTRLEQGSRGLSAGEAQLLAFTRVFLKDPGLVI
                     LDEASSRLDPATERLVERAVDRLLRNRSAIVIAHRLHTVGRAQDILILEKGKTLEYGN
                     RTALAADAASRFAGLLKTGMEEMLV"
     gene            3915..5684
                     /locus_tag="VFH83_12110"
     CDS             3915..5684
                     /locus_tag="VFH83_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487160.1"
                     /translation="MRARLPAWRVILATIRFRPRLWLLNTASLWLLMVFFMIPGLVSR
                     EFFNLVTGDAPTGFTLPTLVAFLFMSEIGGVLGILGAIRTNVPFWVNTMTMLRKNLLS
                     RILQQPGARALPDSPGEAISRFQGDVFEIPLFALWMNDLSSAVFTAAIALSLMFAINP
                     VITAVAILPFSAVAVISFLATSKIEQYRQASRKAAGKVTGFIGELFGAAQAIKVAGAE
                     HGVVARFREINEERRKVSLKDRLFNEVLNSIFNNLANIATGIILVLAAGAMRSHSFTV
                     GDFALFTFYLGFISDLTTFAGLLVARYKQIGVSVERMDRLMGGAEPQELIRFSDVHLK
                     GPLPPVVYARRREGDRLGCLEVSGLTFHHAGTGRGVQDLSFTIRKGTFTVLTGRVGSG
                     KTTTLRVLLGLLPREAGQVLWNGNAVERPDAFFVPPRCAYTAQVPRLFSETLRENVLL
                     GLEAGDEAIREALRLAVLEEDLAGLKDGLDTAVGPKGVKLSGGQLQRTAAARMFVRDP
                     ELLVFDDLSSALDVETESRLWERVFDRPDTTCLVVSHRKPALRRADTIIVLQDGRLEA
                     VGALDELLETCGEMRRLWFGEDR"
     gene            5802..6197
                     /locus_tag="VFH83_12115"
     CDS             5802..6197
                     /locus_tag="VFH83_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET6487161.1"
                     /translation="MISRSGIHAFKAMAVLGGLPPEEYLGAEAIAARIHAPGNYLGKL
                     LRQLSRAGVVEGRKGGNGGFRLARPRETITLLEVLEPIERVVRAEECILGRPRCSAGK
                     PCALHVGWSHVRTAYLEFLRTTKVSELGA"
BASE COUNT          979 a         2081 c         2107 g         1164 t
ORIGIN      
        1 gttcctgcca agaatcgcgg ctgggcgcga gcgggaatct agccctcccg gtggaacgcc
       61 gggtccatcg ggtagaacac cagccccgaa acgggcttgg ggtagaagtg cgtggacttc
      121 cgaggcatgc gctcgtcgtt ctccgcgatg gttatgacgt cgcccagcgc cgtgggattg
      181 aggatgagcg ccacctggat ctccccccgc cgcacccggg caatcgcttc gtccgcgtcc
      241 ttggtatagg cgatgctggt ggaatggttg aggttctctt ccgtgatgcc caggatcccg
      301 tccaggatca gcgtgttcag gatgttgacg tccaggagct tccactccga ggaggtgtcc
      361 agtcggacca gctcctcata ggcctcctcg tcccggaggg tgagcaggta gtagccggtg
      421 gtgttgacga tgtacgcgcc gaacttgtgc tccccggggc tcacgtcccg caggtcccgc
      481 agccagcgtc ttcggatctc cgactcgtcg gcatcccgaa acacgtactt ctttacgtgg
      541 aagtgctcca ggatgtcggc cccgagcttg tagatgctct ccacctggat gccgtggagc
      601 atccggtggg tgggcaggat cgtgagccct tcgtgctcgg agttcacgag gaacatcatg
      661 atgtgctccc acggcgcgtc gggccccgcg tcggggtatt gccgccgcat gcgctcgcga
      721 acggtgagca tggtctggta gcggtgatgg ccgtcggcga tatagatgcg cagccgcgcc
      781 atggtccggc tgatgatccc gcagcgctcc ggatccgtga gccgccagag gcgatggcgg
      841 acgccgatgg gctgctcccg atagtccagg atcggagtct cgcgctccac gtcatccagg
      901 atgttgtcga tgacataggc cttgtcctgg tagaggctcc agatgtactc gaactgggtg
      961 ttggccgtct ccgtgagccg gatgcggtcc cccagcggcc cgtccatcac tttctcgtgg
     1021 ggaaggatcc ggcgctccgc atactcccga agccgcaccg cgccgatgaa ccccttcacg
     1081 cgcttgcgtc cgatgccggg caggtcgaac tcctgctcgt agacccagaa ggacggccgg
     1141 gcggtgcgcc gcagggcgcc gtccgcgcgc caggcggaca gcaggtcgcg agcccgggtg
     1201 tagcggttat tgccgtcgtt gtcgcccggc tccacccggt tcaggatcag ccggatcacg
     1261 ttgtatggat gctgtgcgta ataggcctcg gcctgctcgg cggagatgac gtcgtagggt
     1321 tgcgacaggc acagcccgat gtctttcacc ttcgtgggat cgtagtgcaa ggcgcgaaag
     1381 ggacgcacct cgttgacgct gtcgggcact ccgcctgcct ccttttggcg cacgatagat
     1441 cagaggctct ggacggtcaa gcgcgagggg cttacgcatc accgctgcgg actggtatga
     1501 tggggtcccc agccaacacc cctgcgccgc ggccgccccg cggccgttct tccggttcga
     1561 tcggaggagg ttgtatgaga gtcgccgcgt tcctgggaag tcctcgcccg ggagggaaca
     1621 cggacacact cgcggatgcg atcctggacg gcgctcggga agcgggtctg gagacggacc
     1681 gccacgtgct gcgatccctg cgggtccatc cctgcaccgg ctgcgagctg tgctggacca
     1741 aggggcggcc gtgcatcttc caggacgatg ggacggcgct ctatgactcc gtcgcgtcct
     1801 cggacgtcct tctgttcgcg accccggtct actggtacgg cgcgacggcc atcctgaagg
     1861 cgttcatcga tcgcctggtg gtcttcaacc ggcctcaggg acgtcccctg gttcagggca
     1921 agcgagccgt cctggtgagc gcctgggagg agaaggggat ggaagccgtg gagcccctgg
     1981 tccggcaatt cgagctgagc ttccgcttcc tggagcttcg gttcaccgac cggctcctgg
     2041 tggacgagct ggggcctcgg gacgcggtgc gccagaggcc ggcgctggtt gccagggcgc
     2101 gcgctctggg caaggccctg gtccgggtag agacgtgcat cgatgaaaaa acccgagcaa
     2161 cctgaagggc tgaagatacc tttccgcggg tactgggaca tcctctcccg ctacctcacg
     2221 aaacgatcgg gcaccttcgt gctgctcgcg gtcctgatct gcacggggat cggcctccag
     2281 cttgccaatc cgcagctcct tcggggagtg atcgacgggg ctctggcggg cagaccgtcc
     2341 tccacgctgg tactgtccgc ggtcgcgttc atcctggtag cgctgacgca gcagctcttg
     2401 ggcgtagccg ccgcgtacgt gggagagaac ctcgcgtgga aggccaccaa cgagcttcgc
     2461 tccgatctgg ccgagcactg tgtgtcgctc gacatgaggt accacaacga caccaccccc
     2521 ggagagctga tccagcgcat cgacggggac gtggcggagt tctccaactt cttctcccag
     2581 ctcgtggtcc ggatccttgc caacgttctc ctgatgatcg ggatccttgg ggtcctgtac
     2641 ttcgagaacc cggccatcgg tggcgtgttc accttctttg ccgcgctgac catcggcacc
     2701 ctgagcttcg tgaagaacct ggccgtcggt cccgagaagc tcctgaggga ggcaaacacc
     2761 gacctctccg gcttcctgga ggagaggctg gccggcacgg aggacatccg atcctccggc
     2821 gcggtggact atgtcctgaa cggcctgtac agcctgcacc gcaagatcct gaccttctgg
     2881 aggcaggcgg gcctgatgca cttctgggtc cggatgatct ccggcgtggt cctgaccatc
     2941 gggtacgcgg cggccttcgt cggcgggtac accctctacc gctccggagc catgacggtc
     3001 gggacggtgt tcctcctgat ctactacatg ggcctgctgg cccggccggt ccgggagctc
     3061 gcccagcagg tggacagcct gcagagcgtc ggcgccagcg tcgaacgcat ccgcgagctc
     3121 aaggccctcc gcccgaccat cgtcgatggc cagggtcccg atctcccgtc gcatcagccg
     3181 ctgcgtctgg agttcgagtc cgtggggttc gcctacctcg agggggagca ggtgctggat
     3241 tccctgagct tccgtctgcc gcccggacac atcctgggtc tgctggggag gaccggcagc
     3301 gggaagacga ccatcgcccg gctggtcttc cgtctgtacg agcccacgat ggggcgcgtc
     3361 ctcctggatg gcatcccgct tcccgatacc cggctctcgg atctccgcgc ccgggtggcg
     3421 ttggtgaccc aggacgtgca gctcttccag gcaacggtcc gggagaacat caccttcttc
     3481 gaccggtcca tcccggactc tgccatcctg cgcgccatcg agagcctgga gctggaccag
     3541 tggctggcgg gcctgccgct cggcctggac accagactgg agcagggaag ccgtggtctt
     3601 tccgcggggg aggcgcagct cctggcgttc acgcgcgtgt tcctcaagga ccccgggctg
     3661 gtcatcctgg atgaggcctc ttcacgcctg gacccggcca cggagcggct ggtggaacgg
     3721 gccgtagacc gtctccttcg aaaccgttcc gccatcgtca tcgcccatcg gctgcacacc
     3781 gtcggccgcg cccaggacat cctcatcctg gaaaagggca agacgctgga gtacgggaac
     3841 cggaccgcgc tggccgcgga cgctgcgtca cggttcgcgg gcctcttgaa gaccggcatg
     3901 gaggagatgc tcgtatgagg gcgcggcttc ccgcctggcg cgtgattctg gccaccatcc
     3961 ggtttcggcc ccggctgtgg ctcctgaaca cggcctccct gtggctcctc atggtgttct
     4021 tcatgatccc cggcctcgta tcccgggagt tcttcaacct ggttaccgga gacgcgccca
     4081 ccggcttcac cctgccgacc ctggttgcct tccttttcat gagcgaaatc ggaggcgtcc
     4141 tggggatcct gggcgccatc cgcaccaacg tcccgttctg ggtgaacacc atgacgatgc
     4201 tcaggaagaa cctcctgagc cgcatcctcc agcagccggg cgcccgagcg ctgccggatt
     4261 cgccgggaga ggcgatcagc cggttccagg gcgacgtgtt cgagatcccg ctcttcgccc
     4321 tgtggatgaa cgacctctcc tcggcggtat tcacggccgc gatcgccctg tccctcatgt
     4381 tcgctatcaa ccccgtcatc acggccgtgg ccatccttcc gttctccgcc gtggccgtga
     4441 tctcgttcct ggcgacctcc aagatcgagc agtaccgaca ggccagccgg aaggcggccg
     4501 ggaaggtgac gggattcatc ggggagctct tcggcgcggc ccaggccatc aaggtggccg
     4561 gagcggagca cggtgtcgtc gcgcgctttc gagagatcaa cgaagagcgg cgaaaggtgt
     4621 ccctcaagga ccggctgttc aacgaggtgc tcaactccat cttcaacaat ctggcgaaca
     4681 tcgccaccgg catcatcctg gtcctggcgg ccggcgccat gcgcagccac agcttcaccg
     4741 tgggagactt cgccctcttc accttctacc tggggttcat cagtgacctg accacctttg
     4801 ccgggctcct ggtcgcccgg tacaagcaga tcggcgtgtc ggtggagcgc atggatcggc
     4861 tgatgggcgg cgccgagccg caggagctga tccggttctc cgacgtgcac ctgaagggcc
     4921 ccctgccccc cgtggtttac gcccggcgcc gcgaagggga tcggttgggt tgcctggagg
     4981 tctcggggct gaccttccac catgcgggta ccgggcgcgg cgtgcaggac ctctccttca
     5041 cgatccgcaa ggggacgttc acggtgctta cgggtcgggt cgggtccggc aagaccacga
     5101 cgctccgcgt gctgctgggt ctgctgccta gagaggccgg gcaggtgctg tggaacggca
     5161 atgccgtgga gcggccggat gccttcttcg tgccgcctcg ctgcgcctac acggcccagg
     5221 tgccgcggct cttctcggag acgcttcggg aaaacgtgct cctggggctg gaggcggggg
     5281 acgaggccat acgcgaggcg ctgcggctcg ccgtgctgga ggaggacctc gctggcctga
     5341 aggacggcct ggataccgcc gtgggcccga agggggtgaa gctctccgga ggtcagctcc
     5401 agcgcaccgc ggcggcgcgg atgttcgtcc gggatcccga gctcctggtc ttcgacgacc
     5461 tctccagcgc cctggacgtg gagaccgaat cccggctgtg ggagcgcgta ttcgaccggc
     5521 cggacacgac ctgcctcgtc gtctcccacc gcaagcccgc gctgcgccgc gcggacacca
     5581 tcatcgtgct ccaggacggc cgcctggagg cggtgggcgc gctggacgag ctcctggaga
     5641 cctgcggcga gatgcgccgt ctctggttcg gcgaggaccg gtagggcact cccctcgagc
     5701 gatctgaccc aacgtgcgct cccggcctct tgacgaaatc tctctgccgt gaaatagtct
     5761 aatcgcatga gaatatccga ttcatggatt gagagcagct catgatctca cgatccggca
     5821 tccacgcctt caaggccatg gcggtcctgg ggggcctgcc cccggaggag tacctgggag
     5881 cggaagccat cgccgctaga atccacgctc cgggcaacta cctggggaag ctccttcggc
     5941 agctctcccg cgcaggcgtg gtggaaggcc gaaagggcgg caacggcggc ttccggctgg
     6001 cacgcccacg agagaccatc acgctcctcg aggtgctgga gccgatcgag cgggtcgtcc
     6061 gggccgagga gtgcatcctg ggccggcccc gctgctccgc cggcaagcca tgcgcactgc
     6121 acgtcggatg gtcccatgtg cggacggcct acctggagtt cctccgcaca acgaaagtct
     6181 cggagctggg cgcgtagcgc ccaggggaaa aggcggcgca ggccgcagga ggtacaacga
     6241 tgaaggaccc aagagtccgt gctttgctgc tcttcctgct cgcgatcacc ttcggggtgc
     6301 tcctcttcgg agggtacctg attcatcggg a
//
LOCUS       DASRZS010000496         9014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2965857, whole genome shotgun sequence.
ACCESSION   DASRZS010000496 DASRZS010000000
VERSION     DASRZS010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2965857"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFH83_12120"
     CDS             complement(<1..320)
                     /locus_tag="VFH83_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6487162.1"
                     /translation="MPDATAATTAPAAKKDGEVVLETTDLTKMFGGLTAVESLDLKVH
                     RGEISSLIGPNGAGKTTVFNVVTGIYRPERGTVRFQGANITGAKPHRIVELGVARTFQ
                     NIRL"
     gene            complement(313..1293)
                     /locus_tag="VFH83_12125"
     CDS             complement(313..1293)
                     /locus_tag="VFH83_12125"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6487163.1"
                     /translation="MPLTLPPILKRFALPLVLVVFLVFPFVASDYAVNVAYFFGIYVL
                     LGLSLNIVLGEVGLYDLGHAAYYAIGAYATAILSTRLGIPVLLLLPVSAIAAGLFAYA
                     VTSPIIHLRGDYLCIVTIGIGEIVRLALINNPFDWTGGPNGVYGIATPTLGFLAIDSS
                     LGYYLLVWIVVAITIVGLQRLQRSRIGRAWNCIREDEVAAQATGINVRSHKLLAFVLG
                     AALAGVAGSLYATKLMVVSPASFTFWDSCLMFSIVLIGGMGSIPGVVIGAAAITLFPE
                     LFRSFVNYRMVVFGLAMILMMMFRPGGLWPRRRGGTEARDLIQRLWRRDA"
     gene            complement(1300..2211)
                     /locus_tag="VFH83_12130"
     CDS             complement(1300..2211)
                     /locus_tag="VFH83_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6487164.1"
                     /translation="MRVFLEQLINGLTIGSFYGLIALGYSMVYGVLKLINFAHGDFFA
                     LGSYVGYTLLVFASGFVTAHLGLWGGLLVSVAVAALALAVVGVVVERVAYRPIYPAGR
                     LPAVVSALGASIILQNLIMIVWGPRYQAYPAELVPAIHFQAGGLNLSVLQLVILGVSL
                     LLMVVLYFVIQKSTWGAAVRATALDRDTASLMGINFNRVIRFVFTLGPALGALTGVMV
                     GMYYRQISFTMGWNYGLKAFTATILGGIGNLPGAMFGGLILGVLEMLGSAYISTAWKD
                     VFVFLILILVLIFRPNGLFGEKIAEKV"
     gene            complement(2223..3374)
                     /locus_tag="VFH83_12135"
     CDS             complement(2223..3374)
                     /locus_tag="VFH83_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HET6487165.1"
                     /translation="MKRLVVLSCVLVCACALAAFAGGSQEPVRIGLQGPITGDWAYEG
                     QMAKQATDIAATLINQKGGVLGGRKLEIVVEDDGGQPQTGALAATKISGTKGVVAAVS
                     SYGSSITEPASAIYEKKKMVNIAYGATATRLTEGGKQFFFRTCGRDDSQGKYFAEFVP
                     QKFGAKRIAIMHDNTAFGKGLAENTRDALKSLVDSGKVQIVYYDAITPGEKDFHVPLT
                     KMRETKPDLWYYTGYYAEAALLVTQAREIGITCPFVGGNAAINAEFVKIAGLSVAKGC
                     YMTNEPLPADLPTAEAAEFLKAYKAKYGDIPSSPWPVYAADAVNIIAYALNQTKSTDG
                     TVLADYLRTKVQDVPGITGKIGFTDQGDREGVPMFLYVVDDQGNIVIAK"
     gene            3501..3923
                     /locus_tag="VFH83_12140"
     CDS             3501..3923
                     /locus_tag="VFH83_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487166.1"
                     /translation="MRERKTGDYVGAFFAGVVGLVFVNTVLLWQQYTRGVILGTWADI
                     LWAADLSLIVSIAGNLLLCFYRPARLAALVRVLIAATGLLSIVVFYRVFPLDFSTVVG
                     IWLNTLVKALLIVGMAGGAIGLIVELVRLASAGSAAHA"
     gene            complement(3972..5027)
                     /locus_tag="VFH83_12145"
     CDS             complement(3972..5027)
                     /locus_tag="VFH83_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487167.1"
                     /translation="MASVELIEITKRFGHQTAVDSVSLRVEEGEFVSLLGGSGCGKTT
                     TLRMIAGFESPTSGRLLIRGADVLGVPANRRGVGMVFQNYGLFPHMSAAQNIGFGLRV
                     ARRPAAEIAERVAEMLALIHLDELRDRFPHQMSGGQQQRVALARALAVRPSVLLLDEP
                     LSALDAAIRVRLRTEIRSIQRKLGITAIYVTHDQEEALSISDRVVVMRAGRIEQAGAP
                     AEIYNRPANGFVASFVGASNVLRVTVVDPACGKVSIDGQEISTAKPILKSVGEELHLT
                     IRPESISLRNGTSSNNHLRGTLESVVFLGSVVRLVIRSGAMELIVDSFNDPQAGLPPL
                     GSEVTVGFAPQACLLLP"
     gene            complement(5042..5839)
                     /locus_tag="VFH83_12150"
     CDS             complement(5042..5839)
                     /locus_tag="VFH83_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6487168.1"
                     /translation="MSDPSGIHPRRVWSSLWLSVGALYFLLPLAATFLFSLHGRKGGL
                     SFVAYAHVFADPQFSRTFLFSLEMAAATIAAGLVLMVPTALWVHLRLPRVRPLIEIFT
                     LLPFVIPPIVLVFGLIRVYSRPPLILVSSPVLLVAGYVVLSFPYIYRTVDTGLRAVDL
                     RVLTEAAQSLGARWLTTLFRVVLPSIKVALLSAAFLTFSVVVGELTLAVMLAWPAFGP
                     YMALVGRDLAYEPAALAIISFLLTWGSLLLIQSFSRGLKGTTAGGVH"
     gene            complement(5836..6735)
                     /locus_tag="VFH83_12155"
     CDS             complement(5836..6735)
                     /locus_tag="VFH83_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002967177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6487169.1"
                     /translation="MPVTSLPAEGARRARLPFPSWVGEWAGLLPFFVFALAFMILPAG
                     VLLVGSFQDARGGFTLINVSHLLRPAILRSYLLTIEVSFVSAIGGALFGFLLAYSVTL
                     GGASPAVRGILLTFSGVASNFAGVPLAFAFIATLGRVGLITTLVKAIFGISLYDAGFN
                     LYSFWGLSLTYLYFQLPLMVLIITPALDGLRREWREASQNLGASTAQYWRHVALPILM
                     PSLLSSIILLFGNAFGAYATAYALTGGLINLVPIQIGAQIRGDVLHDPNLGYALALGM
                     VVVMTLSILGYSALQRRAARFMR"
     gene            complement(6741..7868)
                     /locus_tag="VFH83_12160"
     CDS             complement(6741..7868)
                     /locus_tag="VFH83_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6487170.1"
                     /translation="MKKHPRVLLAALLAVGVGTAAAAAPSVLTQAPANFNDLVAAAQK
                     EGQLTVIALPHDWVNYGEAISTFAKKYGIKINELNPDGGSGDEIEAIKANMGNMGPQA
                     PDVIDVGLSFGPDAVASGLLAPYKVRTWSTIPAGVKDPNGYWYGDYYGALAFEVNLDV
                     IKTPPQDWSDLLSPDFKGKVALSGDPRTANQAIMTIGAAALANGGGFDNPKAGLDFFA
                     KLSKVGNFVPLIAVPGTVDSGETPVTIRWDYNAMSNRDKTDGNPNIGVIIPASGVVAG
                     VYVQAISAYAPHPNAARLWEEFLYSDEGQLIWLKGYGHPIRYADLAARNVIPADVAAA
                     MPPASFYAKAVFPTLDQQATLKKYIADNWDSVVNVQVSSNQ"
     gene            8087..>9014
                     /locus_tag="VFH83_12165"
     CDS             8087..>9014
                     /locus_tag="VFH83_12165"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6487171.1"
                     /translation="MSEPLAVPGRGTILVVDDLPENVTLLARMLGRRGYRVRTAHSGP
                     QALLAAAGEPPDLILLDISMPEMDGFEVCRRLKEQPSLREVPVIFISALTETEGLVEA
                     FHRGGVDYITKPFQIEEMEARVETHLKLRRAQAELERYNHYLEQLVDEKVKEISASQL
                     ATIFAMVKLAESRDDVTGTHIWRVREYCRCLATSLQGYSVFRPAVNGSFQRDLYNASP
                     LHDIGKVGITDGILLKPGRHTPQEFDVMKTHTTIGARTLLEVIDQYPRNSLIIMGAEI
                     AHAHHERWDGSGYPRGLSETTIPLAAQIMAVAD"
BASE COUNT         1749 a         2823 c         2943 g         1499 t
ORIGIN      
        1 aacagccgga tgttctggaa cgttcgcgcg acacccagct cgacgattcg gtgaggcttg
       61 gcgccggtga tgttggcgcc ctggaatcgg accgtgccgc gctcaggacg atagatgccg
      121 gtgacgacgt tgaaaaccgt ggtcttcccg gcgccgttgg gcccgatcag gctcgagatc
      181 tcgcctcgat gcaccttcag gtccagcgac tcgacggcgg tgaggccccc gaacatcttg
      241 gtgaggtcgg tagtctccag gacgacctcc ccgtccttct tggcggctgg ggcggtggtt
      301 gcggccgtgg cgtcaggcat ctcgcctcca caggcgctgg atcagatccc gggcctccgt
      361 gccgcctcgc cgtcggggcc agagcccgcc cggccggaac atcatcatca ggatcatggc
      421 caggccgaag accaccatgc ggtagttcac gaaggaacgg aacagctcgg ggaagagggt
      481 aatggccgcg gcgccgatga cgacgccggg gatcgaaccc atgccgccga tcagcacgat
      541 gctgaacatg agacaggagt cccagaaggt gaagctggca ggagagacca ccatgagctt
      601 ggtggcatag aggctgccgg ccacgccggc cagcgccgcg cccagaacga aggccaggag
      661 cttgtgcgac cggacgttga ttccggtcgc ctgggcggcc acctcgtctt ctcggatgca
      721 gttccatgcc ctgccgatcc gcgagcgctg aaggcgctga agcccgacga tggtgatcgc
      781 caccacgatc cagaccagga ggtagtaccc cagggaagag tcgatcgcca ggaaccccaa
      841 cgtgggggtc gcgatcccgt acacgccgtt gggaccgccc gtccagtcaa aggggttgtt
      901 gatcagcgcg aggcggacga tctcgccgat gccaatggtg acgatgcaga gatagtcccc
      961 cctaaggtgg atgatgggcg aggtcaccgc gtaggcgaag aggcccgcag cgatggcgct
     1021 gacggggaga agaagcagga ccggaatccc cagccgggtg ctgaggatgg ccgtggcgta
     1081 ggcgccgatc gcgtaatacg cggcgtgccc gaggtcgtac aacccgacct cgccgagcac
     1141 gatgttcagg ctgaggccca ggagcacgta gatgccgaag aagtaggcaa cgttcacggc
     1201 gtagtcgctg gctacgaagg gaaataccag gaacaccacc aggaccaggg gcagggcaaa
     1261 ccgcttgagg atgggcggca gggtgagagg catgggagac tagaccttct ccgcgatctt
     1321 ctcgccgaag aggccattgg gccggaaaat caggaccagg atgaggatca ggaaaacgaa
     1381 cacgtccttc cacgccgtgg agatgtaggc gctgcccagc atctcgagga cgcccaggat
     1441 caggcccccg aacatcgcgc ccggcaggtt gccgatgccg cccaggatcg tcgccgtgaa
     1501 cgccttgagg ccgtagttcc agcccatggt gaagctgatc tgccggtagt acatgcccac
     1561 catgacgccg gtcagcgcgc ccagggcggg acccagcgtg aaaacgaacc ggatcacccg
     1621 gttgaagttg atccccatga gggaggcggt gtcccgatcc agggccgttg cccgcacggc
     1681 ggcgccccag gtggacttct ggatcacgaa gtagagaacc accatgagga gcagcgacac
     1741 gcccaggatg acgagctgca gcaccgacag gttcagtcca ccggcctgaa agtggatggc
     1801 agggaccagt tccgccgggt aggcctggta tcgaggtccc cacacgatca tgatgaggtt
     1861 ctgcaggatg atcgaggcgc ccagcgctga gaccacggcg ggaagccgac ccgcaggata
     1921 gatcgggcga taggccacgc gttccacgac gacgcccacc acggccaggg cgagggcggc
     1981 cacggccacg gagaccagga ggccgcccca caaccccagg tgggccgtca cgaatccgga
     2041 ggcgaacacc agcagggtgt agcccacgta cgagcccagg gcgaagaaat cgccatgagc
     2101 gaagttgatc agcttcagga cgccatagac catcgagtag cccagggcga tcaacccgta
     2161 gaacgagccg atcgtcaggc cgttgatcaa ctgctcgaga aagacccgca tcgagaaacg
     2221 atctacttgg cgatgacgat gttcccctgg tcgtctacga cgtacaggaa cataggcacg
     2281 ccctcacggt cgccctggtc cgtgaacccg atcttgcccg tgatgcccgg cacgtcctgc
     2341 accttggtgc gcaggtagtc cgccagcacg gtgccgtccg tggacttggt ctggttgagg
     2401 gcgtaggcga tgatattcac cgcatccgcg gcgtacaccg gccacgggct ggaggggatg
     2461 tctccgtact tcgccttgta ggccttcagg aactcggccg cctccgcggt cggcagatcc
     2521 gcggggagcg gttcgttggt catgtagcaa cctttggcta ccgacagacc ggcgatcttc
     2581 acgaactccg cgttgatagc cgcgtttccc ccgacgaacg ggcaggtgat cccgatttcg
     2641 cgcgcctgag tgaccaggag ggccgcctcc gcgtaatagc cggtgtaata ccagagatcc
     2701 ggcttggtct ctcgcatctt ggtgagaggc acgtggaagt ccttctcccc cggcgtgatg
     2761 gcgtcatagt acacgatctg gaccttgccc gagtccacca gggacttcaa cgcgtcccgg
     2821 gtgttctccg ccaacccttt tccgaacgcc gtgttgtcat gcatgatggc gatccgcttg
     2881 gcgccgaact tctggggaac gaactcggca aagtacttgc cctgagagtc gtcgcggccg
     2941 caggttcgga agaagaactg cttgccgccc tccgtgagcc gggtggccgt ggcgccatag
     3001 gcgatgttga ccatcttctt cttttcatag atggcagagg cgggctcggt gatcgaggag
     3061 ccataggagg agaccgcggc gaccacgccc ttggtcccgc tgatcttggt ggccgccagc
     3121 gcgcccgtct gaggctggcc cccgtcatcc tccaccacga tctccagctt ccgccctccg
     3181 agcactccgc ccttctggtt gatgagcgtg gcggcgatgt ccgtggcctg ttttgccatc
     3241 tggccttcgt aggcccagtc acccgtgatg ggcccctgaa ggccgatgcg cacgggttcc
     3301 tggctgccac ccgcgaaggc tgccagggcg caggcgcata cgagcacgca cgaaagcacc
     3361 actagcctct tcatgggatc ctcctttgcc ggggaatgtg cgagatgcaa aggttatagt
     3421 ctgccccgcc cggggtgtca aggcaaaggg attcaggtgt tgcatatact tgcagggtgg
     3481 gacgtgcgaa ggagggaagc atgagggagc ggaagacggg agactacgtc ggggcgtttt
     3541 tcgccggcgt tgtcgggctg gtgttcgtca acaccgtgct gctgtggcag cagtacaccc
     3601 gaggagtgat tctgggtacc tgggcggaca tcctgtgggc agcggatctt tcgctgatcg
     3661 tttcgatcgc gggcaacctt ctgctctgct tctaccggcc tgcccggctg gccgccctgg
     3721 tccgggtcct catcgctgcc acggggcttc tgtcgatcgt cgtattctac agggttttcc
     3781 cgctggactt ctcgacggtc gttggcatct ggctgaacac cctcgtgaag gctcttctga
     3841 tcgtgggcat ggcgggaggg gcgatcggcc tgatcgtcga gttggtgcgg ttggccagcg
     3901 cgggctccgc cgcgcacgcc tgacggcgga cgaggactcg ccgcccggca gccccgttgg
     3961 aggccgccgg ttcagggcag caaaaggcag gcctggggag cgaagccaac cgtgacctcc
     4021 gagccaaggg gaggcaggcc ggcctgggga tcgttgaagg agtcgacgat gagctccatg
     4081 gcgccgctcc ttatcaccag ccttaccacg gagcccagga acaccacgct ctccagcgtt
     4141 ccgcgcaggt ggttgttgga gctggtcccg ttccgaaggc tgatcgattc agggcggatg
     4201 gtgaggtgaa gctcttcccc gaccgacttc aggatgggct tcgccgtgga gatctcctgg
     4261 ccgtcgatgg agacctttcc gcacgccgga tccacgaccg tcacgcgaag gacattcgat
     4321 gctcccacga aggaggcgac gaagccgttg gccggccggt tgtagatctc cgctggagcg
     4381 cccgcctgct cgatccgccc ggccctcatc actacgacgc ggtcggagat ggacagcgcc
     4441 tcctcctggt cgtgggtgac gtagatcgcc gttattccga gcttgcgctg gatggaccgg
     4501 atctccgtgc gcaagcggac tcggatggcc gcatccagcg ctgagagggg ctcgtccagc
     4561 aggagaacgg aaggtcgaac ggcgagggct cgggccaggg ctacccgctg ctgctggcca
     4621 ccggacatct ggtgcgggaa gcgatccctc aactcatcca gatggatcaa ggcaagcatc
     4681 tcggccacgc gctcggcgat ctccgctgct ggccgtcgag ccacccgcag tccgaagccg
     4741 atgttctgcg cggcgctcat atggggaaag agcccgtagt tctggaacac catccctacg
     4801 ccgcgtcggt tggccggtac gcccaggacg tccgccccgc ggatgagcag tctgccgctc
     4861 gtcggggact cgaagcccgc gatcatccgc agcgtcgtgg tcttgccgca accggatcct
     4921 cccagaagtg agacgaactc cccctcttcc accctcagag agacggagtc cacggccgtc
     4981 tggtgaccga accgcttggt tatctcgatc agctcgacag aagccatacc tcgtctctct
     5041 ttcagtgcac gccgcctgcg gtggtgccct tcagccctcg gctgaaggac tggatcagga
     5101 gcaacgatcc ccaggtcagc aggaagctga tgattgccag cgcggccggc tcatacgcca
     5161 ggtcccgacc gaccagcgcc atgtagggtc cgaacgccgg ccatgccagc atgaccgcca
     5221 gggtgagctc tccaacgaca accgaaaagg tcaggaaggc ggcgctcagg agcgccacct
     5281 ttatgctggg caggaccacg cggaacaggg ttgtcagcca ccgcgccccc aggctctggg
     5341 ctgcctcggt caggacccgc agatcgaccg cccgtagtcc ggtgtccacg gtcctgtaga
     5401 tgtatggaaa ggagagcacg acatagccgg ccaccaggag caccgggctg ctcaccagga
     5461 tgagcggcgg ccggctgtac acgcggataa gcccaaagac cagcacgatc ggcgggatga
     5521 caaagggcag gagcgtgaag atctcgatca acggcctcac ccgcgggagt cgaaggtgga
     5581 cccagagcgc cgtgggtacc atgaggacaa gaccagccgc gatcgtagcg gcggccatct
     5641 ccagcgagaa aaggaacgtc cgggaaaact gcgggtccgc gaacacgtgc gcgtaagcga
     5701 caaagctcaa tccacccttc ctgccgtgca acgagaacag gaatgtggcg gcaagcggga
     5761 ggaggaaata caacgcgcct acggaaagcc agaggctgga ccacaccctt cgcggatgaa
     5821 tcccgctggg atcgctcatc gcatgaacct cgccgcccgc cgttggagcg cggaatagcc
     5881 caggatgctc agggtcatca cgaccaccat acccagggcc agcgcgtagc ccaggttcgg
     5941 gtcgtgcagc acatccccgc ggatctgggc accgatctgg atcggcacca ggttgatgag
     6001 gcctccggtc agcgcgtagg cggtggcgta cgcgccgaag gcgttgccga agaggaggat
     6061 gatcgatgag agcagggacg gcatgaggat gggcagcgca acgtggcgcc agtactgtgc
     6121 ggtcgacgct ccgaggttct gactggcctc gcgccattcc cttcgaagac cgtccagggc
     6181 cggcgtgatg atcaggacca tgaggggaag ctggaagtaa aggtacgtca ggctcagtcc
     6241 ccagaagctg tagaggttga accccgcgtc gtacaggctg ataccgaaga ttgccttcac
     6301 cagggtcgtg atcaaaccca cccgccccag ggtggcgatg aatgcgaacg ccagcggtac
     6361 ccccgcgaag ttggacgcca cgccgctgaa ggtgagcaga atcccgcgga cggcgggaga
     6421 tgctcctccc agcgtaacgg agtacgccag caggaaaccg aacagggcgc ccccgatcgc
     6481 ggatacaaag ctgacctcga tggtcagaag gtacgaccgc aggatagcgg gccgaagcaa
     6541 gtgggagacg ttgatgagag tgaaaccgcc gcgggcgtcc tgaaagctcc caaccagcag
     6601 caccccggca gggaggatca tgaaggcaag agcgaagaca aagaacggca gcagccccgc
     6661 ccactcccct acccacgacg ggaaaggaag gcgcgcacgg cgcgcgcctt ccgccgggag
     6721 gctagtgacg ggcatcaggc ctactgattc gaggaaacct ggacgttgac cacggagtcc
     6781 cagttatccg caatgtactt tttgagcgtt gcctgctgat ccagggtcgg gaaaacggct
     6841 ttcgcgtaga acgaggccgg aggcatggca gcggcaacat ccgccgggat cacgttccgc
     6901 gccgcaagat ccgcatagcg gatggggtgt ccgtaaccct tcagccagat gagctgaccc
     6961 tcgtcggagt acaggaactc ctcccaaagc cgcgcggcgt tcgggtgcgg agcatacgcg
     7021 ctgatggcct ggacgtacac gccggctacc actccggagg cggggataat caccccgatg
     7081 ttggggttgc cgtcggtctt gtcgcggttg ctcatggcgt tgtagtccca gcggatcgtc
     7141 accggggtct cacccgagtc gaccgtgcca ggaaccgcga tcaggggaac gaagttcccc
     7201 accttgctga gcttggcaaa gaagtccagc cccgccttcg gattgtcgaa cccgccaccg
     7261 ttggccaggg cggcggcgcc gatggtcatg atggcctggt tggcggttct gggatcgcct
     7321 gagagggcca ctttgccctt gaagtccggt gaaagaaggt ccgaccagtc ctggggcgga
     7381 gtcttgatca cgtcaaggtt gacttcgaac gccagagcgc cgtagtaatc cccgtaccag
     7441 tacccgttgg ggtccttgac ccccgcaggg atcgtgctcc acgtgcggac cttgtaagga
     7501 gccaggagcc cggaggccac ggcatccggg ccgaaggaga ggccgacgtc gatcacgtcg
     7561 ggagcctgag ggcccatgtt gcccatattc gccttgatgg cctctatctc gtctccggaa
     7621 ccgccgtccg gattgagctc gttgatcttg atgccgtatt tcttggcgaa ggtcgagata
     7681 gcctccccat agttcaccca gtcgtgggga agcgcgatca ccgtcagttg gccttccttc
     7741 tgggcggcgg ctaccagatc gttgaagttc gccggtgcct gggtgagaac ggacggggca
     7801 gccgcggcag cggtcccgac tcccacggcc aggagcgcgg ccagaagaac cctcggatgc
     7861 ttcttcatat gctctccttt ctcgggtaca ccgggcgctg ccggtgaccg ggtcaggaga
     7921 tgagtagaag aaaaccgtta ggattccacg aatccggcgt tagctttcgg ttaatagcgc
     7981 ccaagcgcgc caggaacgac cgtcccggtt cggtcgtccg ggcccgcggt agacttcggc
     8041 tttgacagcc ctggaccctc gggatactat ggaacccacg gcacccatgt cagaaccctt
     8101 ggctgtcccc ggtcggggga cgatcctggt cgtcgacgat ctccccgaga atgtgaccct
     8161 gttggcccgg atgttgggtc ggcgaggcta ccgggttcga accgcgcact caggtccgca
     8221 ggcgctcctg gcggccgcgg gggagccgcc cgacctgatc ctcctggaca tcagcatgcc
     8281 ggagatggac gggttcgagg tctgtcggcg gctcaaggaa cagccttctc tccgagaggt
     8341 tccggtgatc ttcatcagcg ccctgacgga gaccgagggt ctggtggagg cgttccaccg
     8401 aggaggcgtg gactacatca ccaagccttt ccagatcgaa gagatggaag cccgcgtcga
     8461 gacccatctg aagcttcgcc gcgcccaggc cgagctcgag cgctacaacc actacctgga
     8521 gcagctcgtc gacgagaagg tcaaggagat ctccgcctcc caactggcca ccatcttcgc
     8581 catggtgaag ctcgcggaat cccgggacga cgtaaccgga acccacatct ggcgcgttcg
     8641 agagtattgc cgttgcctgg cgacctcgct ccagggctat tccgtgttcc gacccgccgt
     8701 gaacggttcc ttccagcgcg atctgtacaa cgcgtcaccg cttcacgata tcggcaaggt
     8761 agggatcacg gacggcatcc tgctcaaacc cggcaggcac actccgcaag agttcgatgt
     8821 catgaagacc cacaccacga tcggggcccg gaccctgctg gaagtgatcg accagtaccc
     8881 ccgcaactcc ctgatcatca tgggtgcgga gatcgcccac gcacaccacg agcgttggga
     8941 tggcagcggc taccctcggg ggctgtccga gaccaccatc ccgctggccg cccagatcat
     9001 ggccgttgcc gacg
//
LOCUS       DASRZS010000497         6314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3737739, whole genome shotgun sequence.
ACCESSION   DASRZS010000497 DASRZS010000000
VERSION     DASRZS010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6314
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3737739"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFH83_12170"
     CDS             <1..475
                     /locus_tag="VFH83_12170"
                     /inference="COORDINATES: protein motif:HMM:NF015318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DOMON domain-containing protein"
                     /protein_id="HET6487172.1"
                     /translation="SASLAKTTTAPKVDGTLNAKEYSYTLQQPSVQLGMSWVDDTLYV
                     AVTGQTTGWVAVGLGSPRMDGAVLYLGFVADGKGQMKVQQGTGHRHGDTSSDTPTQFA
                     LKEANGQTTMELALKADGFIAASQNKLDVILAYGTADSFNSMHRAHFTTTVDITP"
     gene            438..767
                     /locus_tag="VFH83_12175"
     CDS             438..767
                     /locus_tag="VFH83_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487173.1"
                     /translation="MRISRPPSTSPRNGRAVPIALRVNLLIVVSLVLGMGVGVTWLAL
                     TQYTAAVASTDTALRRQSQIVYYSIKNLMLPGEAPIARSFVGDIQLNAGLSFELTLYR
                     RDGVQTR"
     gene            764..889
                     /locus_tag="VFH83_12180"
     CDS             764..889
                     /locus_tag="VFH83_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487174.1"
                     /translation="MIKRPEDPRAKGGQQNPYGDKNPQTEFRDYTYVLYVPDTGS"
     gene            898..1263
                     /locus_tag="VFH83_12185"
     CDS             898..1263
                     /locus_tag="VFH83_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET6487175.1"
                     /translation="MPVIRIHGTDRSFPAKLTMSILNTLLSQRYPIDTVCGGKAQCGR
                     DIIRIVSGARYLSPCRERERARLDELARLGEPSGPDIRLACQTYVRGDVEIEVIHHRE
                     PGVGAPRPPLPGRPPLPGA"
     gene            1298..3145
                     /locus_tag="VFH83_12190"
     CDS             1298..3145
                     /locus_tag="VFH83_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487176.1"
                     /translation="MGRFARNLLLPGILVVLLSCGEQPFLLSTASETTDLQIQSVSDG
                     QVVSSGKDLQLTIATQSADKHQDLEMEVTITSASGASVYHLRQAIPDLNKAVPLTAPD
                     LPVGQYRMDVVVYSAGDAIQKKSSSFFVAPDVWRIAGIKSFPPVITAAANVLLRAELE
                     IPSGADPYLRWSWKGKIISRGQLSKGLGQILWQTPADDGVYTVLLEMFPSAPTDGTDF
                     PFTSSLSLSTDIYVSGKGVAGKGDLADKASYLSLLRLQASLVDDGTGAARGHPSATAV
                     GNPQIVAAGTSFGYHLDGASGFQLPWFALPVTGGSLQPFTVSVGLSLEDFQGSGNLVN
                     AVASGGLTVQIVVDGVTRMPSARIATGADTGIEIPWTGPGLERNQRYLLSLSVAPQRS
                     TLTAQWFLDGTQVSVWTGAFSSPEASQDGQTTIGGSGGIVGVVDEFGVYDKDASNRAA
                     TDPDLFHRAEESQLGTAMVLADGFDGLYLSDGFVSSGGVVGGGALTLPPDASVSLPAV
                     GLAGAPLDVSVALDAASTPSVGLRLFWAGETDPTAESAIAVNGSRLQLRVAADGRTVT
                     VYPGPDQATLSLPGGTRANAKLVIQLGNPKTSQGPVILQQVLVTAAPTG"
     gene            complement(3135..4013)
                     /gene="era"
                     /locus_tag="VFH83_12195"
     CDS             complement(3135..4013)
                     /gene="era"
                     /locus_tag="VFH83_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET6487177.1"
                     /translation="MLKSATVAVVGRPSSGKSTLVNALCGGKVSIVSPVPQTTRNRVR
                     GILTAPAGQLVFIDTPGFHLSEKKLNQYMGSLVNAALAEVDVVLYLLDGTREPGTEER
                     ALIDAIRTAAKPAVACVSKKDLESESRELVRRVAAQELPGARLLEASALTGEGLDGLR
                     EALFQLSPEGEQLYPPDYYTDQTPEFRISEIVREKAMLQTREEVPHALYVRIEDLEMR
                     QEGAFLQARGFICVERESQKGIVVGRGGEKIHTIVSEAERELAEIFPYPVRLDIRVKV
                     DREWRKRDPLLRRLIQ"
     gene            complement(4058..5653)
                     /locus_tag="VFH83_12200"
     CDS             complement(4058..5653)
                     /locus_tag="VFH83_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6487178.1"
                     /translation="MPELAELLTRLRHEEEPLARADRILEWAIEELGLQGVALIRAEP
                     SSAVGLRVLRATGSLCGLSAEVVNDLLPAGLPAQSCILALPAALPTVFPAKAVGYGSN
                     GGSSIPDALYCAPLNRQNGVPWSALVLDSNDLSQLTRDNDERLQMASSLLRDALENEE
                     LRRQLRMARTRNGVFALGDSTDGHPVAPEIDSLVDGLQLPLYLCDSHGKFLYASAAFL
                     RLTGFATVEALSRATGLFGDPVKRRQELETVLSAGKVSAVPITVTAADGKRLQIQESA
                     LSMGDRVLGVFFDVSDLMEANKELKDSLQVQEFLNDSIIAGTRMLRRTQGASIRALAR
                     LAEYRNPETGYHLQRICEYARLLAVQVYERAPYSFRITREYGDDISLSSMLHDIGKVS
                     IPDSILLKPGRLDSDEWEIMKRHTVFGWEVLSKADKELGEQSFLTLAATIALSHHERF
                     DGTGYPRGIAGENIPLSARISSLADVYDALTTARPYKEPWSHERAMEEIVRQSGCQFD
                     PVLVGILQEVHDQFAEVHRLFPG"
     gene            complement(5824..>6314)
                     /locus_tag="VFH83_12205"
     CDS             complement(5824..>6314)
                     /locus_tag="VFH83_12205"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HET6487179.1"
                     /translation="SFADPFCSGAREVIARVLKTRGQACHPLGTYVCMEGPAFSTRAE
                     SELHRSWDAALIGMTALPEARLAREAEICYAMIAMVTDYDCWRPNEGSVTVEMVVSTV
                     RKNTAAIQAVLPDLCSALASRGDCSCHHAAEGAIMTHPSVIPHDARRRLSLFYGRFWK
                     DA"
BASE COUNT         1058 a         2113 c         2080 g         1063 t
ORIGIN      
        1 gtcagcgtct ctggccaaga ccacgacggc acccaaggtc gacgggacgc tcaacgccaa
       61 ggagtactcc tataccctgc agcagccgtc cgtccaactg gggatgagct gggtggacga
      121 caccctctac gtggctgtga ccgggcagac gactggatgg gttgcggtgg gtctcggctc
      181 gccgcgcatg gacggcgcgg ttctgtacct gggctttgtt gccgacggca aggggcagat
      241 gaaggtccag caggggacgg gtcaccgtca cggggacacg agctccgata cgccgacgca
      301 gttcgcgctg aaagaggcca atggacagac cacgatggag ctggcgctga aggcggatgg
      361 gttcatcgcg gccagccaga acaagctcga cgtgatcctg gcgtacggaa ccgcggactc
      421 gttcaactcc atgcaccgtg cgcatttcac gaccaccgtc gacatcaccc cgtaacggcc
      481 gggcggtccc gatcgccctt cgggtcaacc tcctcatcgt cgtcagcctg gtgctgggga
      541 tgggggtcgg cgtcacctgg ctcgccctga ctcagtacac cgccgccgtg gcatccacgg
      601 acacggcgct tcggcgccag tcgcagatcg tctactactc catcaagaac ctcatgcttc
      661 cgggcgaggc tcccatcgcc cggagcttcg tgggggacat ccagctcaac gccgggctca
      721 gcttcgagct cactctgtat cggcgagacg gcgtgcagac gcgatgatca agcgccccga
      781 ggatccgcgg gcaaagggcg gtcagcagaa cccatacgga gacaagaacc cgcagaccga
      841 gttccgcgac tacacctacg tcctttacgt tccggacacc ggcagctgag aacgacgatg
      901 cccgtcatac ggatccacgg aaccgatcgc tccttcccgg ccaagctgac catgtccatc
      961 ctgaacacgc ttctctccca gcgctatccc atcgatacgg tgtgcggagg caaggcgcaa
     1021 tgcggccggg acatcatccg catcgtctca ggcgcccggt atctgagtcc ctgccgggag
     1081 cgggagaggg cccggctgga cgagctggcg cgcctcggag agccatcggg acccgacatc
     1141 cgtctggcct gccagacgta cgtccggggg gacgtcgaga tcgaagtgat ccaccatcgg
     1201 gagcccggcg tcggggcccc ccggccccct ctgcccggac gcccccctct gcccggcgct
     1261 tgattatgcc gaccgtagtg gtatactagc cctgacgatg gggagattcg ctcgaaatct
     1321 actccttcca ggcattctcg tggtgttgct ttcctgcgga gagcagccat tcctcctgtc
     1381 cacggccagc gaaacaacgg acctgcagat ccagtccgtg tcggatgggc aggtcgtctc
     1441 gagcggcaag gatctccagc tcaccatcgc cacccaaagc gcggacaagc atcaggacct
     1501 ggagatggag gtgaccatca cctccgcctc cggcgcgagc gtctaccacc tgcggcaggc
     1561 gataccggac ctgaacaagg ctgtgcccct gactgcgccg gaccttcccg tgggtcagta
     1621 tcgcatggac gtcgtcgtct actccgcggg agacgcgatc cagaagaagt cctccagctt
     1681 cttcgtggct ccggacgtct ggcgtatcgc cggcatcaag agcttcccac cggtcatcac
     1741 cgccgccgcc aacgtgctgt tgagggcgga gttggaaatc cccagcgggg cggacccgta
     1801 tcttcgctgg tcctggaagg gcaagatcat ctcccgtggt cagctcagca agggactggg
     1861 gcagatcctc tggcagaccc cggcggacga tggggtctat accgtcctgc tggagatgtt
     1921 ccccagcgcg ccgacggatg gaacggactt cccgttcacc tcgtcgctct ccctctccac
     1981 ggacatctac gtctccggaa aaggcgttgc cggcaaggga gacctggcgg acaaggcctc
     2041 gtacctgtcc ctcctccgcc tgcaggcgag cctcgtggac gatggcaccg gcgccgcccg
     2101 gggccaccct tcggccaccg cagtcgggaa cccccagatc gtggcggccg ggacctcttt
     2161 cgggtatcac ctggacggcg cttctggctt ccagcttccg tggtttgccc ttccggtaac
     2221 cgggggcagc ctgcagccgt ttacggtctc cgtgggcctg agtctcgagg acttccaggg
     2281 cagcggcaac ctggtgaacg cggtggcctc cggcgggctt acggtgcaga tcgtcgtgga
     2341 cggtgtcacc cgcatgccgt cggcccggat cgccaccggt gcggacaccg gtatcgagat
     2401 cccctggacc gggccgggcc tggagaggaa ccagcgatac ctcctctcgc tgtcggtcgc
     2461 gccccagcgc tccacgctca cggcccagtg gttcctggac gggacccagg tgtcggtctg
     2521 gaccggcgcg ttctcctctc ccgaagcgag ccaggatggt cagaccacga tcggcggcag
     2581 cggcggcatc gtgggcgtgg tggatgagtt cggcgtatac gacaaggatg cgtccaacag
     2641 ggccgccacc gatcctgacc tgttccaccg cgcggaggaa agccagctcg ggaccgccat
     2701 ggtgctggcg gacggtttcg acggcctcta tctttcggat ggtttcgtct ccagcggtgg
     2761 cgtggtgggc ggtggagccc tgacccttcc gccggacgcc tcggtgagcc tcccggccgt
     2821 cgggctggcc ggcgcgccgc tggatgtgtc cgtggcgctt gacgctgcgt ccactccgtc
     2881 cgtcggcctg cggctcttct gggcgggaga gacggacccc accgcggagt ccgctatcgc
     2941 ggtcaacgga tcgaggctgc agctccgagt ggcggcggac ggccgaaccg tgacggtgta
     3001 tcccggaccg gatcaggcga cgctcagcct cccgggaggg acgcgagcca acgcgaagct
     3061 cgtgatccag ctcggaaacc ccaagacctc tcagggcccg gtcatcctgc agcaggtgct
     3121 ggtgaccgcg gctcctactg gatgagccgc ctcaggagcg gatcccgctt cctccactcc
     3181 cggtcgacct tcacgcggat atcgagcctc accggatagg ggaagatctc ggcgagctcc
     3241 cgctccgcct cggacacgat cgtgtggatc ttttccccgc cccgccccac cacgataccc
     3301 ttctgggact cccgctccac gcagatgaat ccacgcgcct gcaggaaggc tccttcctgg
     3361 cgcatctcga ggtcctcgat gcgcacatac aacgcgtggg ggacctcctc tcgggtctgg
     3421 agcatcgcct tctcacggac gatctcggag atgcggaact ccggcgtctg atcggtgtag
     3481 tagtccggcg ggtagagctg ctcgccctcc ggtgagagct ggaagagcgc ctcccgcagc
     3541 ccatcgagcc cctcccccgt gagagcggag gcttccagca gccgcgcgcc gggaagctcc
     3601 tgcgccgcga cccgccgaac cagctcccgc gactcgctct ccaggtcttt cttgctgacg
     3661 cacgcgaccg ccggcttggc tgccgtccgg atcgcgtcga tgagcgcccg ctcctcggtg
     3721 ccgggctccc gggtgccgtc caggagatag aggaccacat ccacctcggc aagagccgcg
     3781 ttgaccaggc tccccatgta ctggttcagc ttcttctcgg agaggtggaa gcccggcgtg
     3841 tcgatgaaga cgagctgtcc ggcgggggcg gtcaggatgc cgcggacccg gttgcgggtg
     3901 gtctgaggaa ccggcgagac aatggagacc ttgccgccgc acagcgcgtt gaccagcgtg
     3961 gatttcccgg aagaggggcg gcctaccacg gcaaccgtgg ctgacttcag catggtacga
     4021 cccaccggat cgtcgcggaa cgtcgggacg ggcccgctca accggggaag agccggtgca
     4081 cctcggcgaa ctgatcgtgc acctcctgga ggatccccac gagcacggga tcgaactggc
     4141 agccggactg gcggacgatc tcctccatgg cgcgctcgtg gctccagggc tccttgtagg
     4201 gcctcgcggt ggtcagcgcg tcatagacgt cggccaggga ggagatccgc gccgagaggg
     4261 ggatgttctc cccggcgatg ccccgcggat acccggtgcc gtcgaacctc tcgtggtggg
     4321 acaaggcgat ggtcgccgcc agcgtgagga aggactgctc ccccagctcc ttgtccgcct
     4381 tgctcaggac ctcccatccg aacacggtat ggcgcttcat gatctcccat tcgtccgaat
     4441 cgagcctgcc gggcttcagg aggatgctgt ccgggatgct caccttcccg atgtcgtgca
     4501 gcatgctcga gagcgagatg tcatcaccat actcccgggt gatccgaaac gaataggggg
     4561 cgcgctcata gacctgcacg gccaggagac gggcatactc gcagatgcgc tggaggtgat
     4621 agccggtctc ggggttgcgg tattccgcga gccgggcgag ggcgcggatg gaggcgccct
     4681 gggtcctgcg cagcatgcgg gtccccgcga tgatgctgtc gttgaggaac tcctggacct
     4741 ggagggaatc cttgagctcc ttgttggcct ccatcaggtc gctcacgtcg aagaagactc
     4801 caaggacccg atcccccatg gagagcgcgg attcctggat ctgcagccgt ttgccgtccg
     4861 cggcggtcac ggtgatcgga acggcgctca ccttaccggc ggacagcacc gtctccagct
     4921 cctgcctccg tttcaccgga tcgccgaaca ggcccgtggc gcggctcagg gcctccacgg
     4981 tcgcgaagcc cgtgaggcgg aggaaggcgg ccgaagcgta gaggaacttc ccgtggctgt
     5041 cgcacaggta caggggaagc tgaagcccgt ccaccaggct gtcgatctcc ggtgccacgg
     5101 gatggccgtc cgtggagtct cccagggcga agacgccgtt tcgggtgcgg gccatgcgga
     5161 gctggcgtcg cagctcctcg ttctccagag cgtcccgaag cagagaggag gccatctgca
     5221 gccgctcgtc gttgtctcgg gtgagctggc tgaggtcgtt ggagtccagg accagggcgc
     5281 tccacgggac gccgttctga cgattcaggg gggcgcagta cagcgcgtcc ggaatggatg
     5341 agcccccgtt ggatccgtac cccaccgcct ttgccggaaa gaccgtggga agcgccgccg
     5401 gcagggccag gatgcaggac tgggcgggga gcccggccgg aaggagatcg ttcacgacct
     5461 ccgccgacag tccgcagagg gagcccgtgg cccggaggac ccgcaggcca accgcggacg
     5521 aaggctcggc gcgaatgagc gcgaccccct gcagcccgag ctcctcgatt gcccattcca
     5581 ggattcgatc ggcgcgcgca aggggctcct cctcatgacg gagccgcgtc aagagttcgg
     5641 cgagctctgg cactcttcct cccgtgcaga agtgtcctat tctgattgcc tgacgtagag
     5701 aagggtaacg cgcttttgtg gtgtcgtcag tgcagccgag cctctcgcgg cgattattcg
     5761 cccttccggc ccgactctcc gagtgactgg cttctcccaa acgactggcg gtcctcccgg
     5821 cgactaggcg tccttccaga aacgtccgta aaagagcgaa agtctcctgc gggcgtcgtg
     5881 cggaatgacc gacggatggg tcattatggc gccctcggcc gcgtgatggc aggagcagtc
     5941 gccgcgcgac gcgagggcgg agcagagatc cggcaagacc gcctggatgg ccgcggtgtt
     6001 tttccggacc gtggaaacca ccatctccac ggtcacggag ccttcgttcg gcctccagca
     6061 gtcatagtcg gtcaccatgg cgatcatggc gtagcagatc tcggcctcgc gggccaggcg
     6121 cgcttcgggc agggcggtca tcccgatgag agccgcgtcc cacgaccggt gcagctccga
     6181 ttccgcgcgc gtggagaagg ccggaccttc catgcacacg taggtgccca aggggtgaca
     6241 ggcctgtccc cgggtcttga ggacccgggc gatgacctcc cgagcgccgg agcagaacgg
     6301 atcggcgaac gaga
//
LOCUS       DASRZS010000498         3350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5660529, whole genome shotgun sequence.
ACCESSION   DASRZS010000498 DASRZS010000000
VERSION     DASRZS010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5660529"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VFH83_12210"
     CDS             <1..1117
                     /locus_tag="VFH83_12210"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET6487180.1"
                     /translation="GLLQDGFPGTTAILYRMNAQSRHFEDRFRVEGIPYRLIGTVRFY
                     SRQEVKDALAYLSLLVNPRDEVAFRRIANTPSRGLGPASVEKIVALRGPGESVEDACR
                     RAQVPPRARLGVRELLGCLQECAARLEELPLSELARLLLTRSGLYEHYRQRDRSDGTS
                     KSANLEEMVSAMAPYGAGAEALGAFLESVALANPADEAEQSGEEPKVTLITLHNTKGL
                     EFDRVIISGLEEGIFPHDASSLSAEDLEEERRLFYVGITRARERLTMTWCLRRRIFGK
                     VIENPPSRFLDEIPKEQINRVESEAAAFLLGRGAGSEADDYPPGAGVFHDEYGPGVVE
                     RTWYTDGSLLVQVRFQSGRVAKFLPRYTRLERIGFDE"
     gene            1110..1907
                     /gene="folE2"
                     /locus_tag="VFH83_12215"
     CDS             1110..1907
                     /gene="folE2"
                     /locus_tag="VFH83_12215"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HET6487181.1"
                     /translation="MSNDRTQLKDIQGGPDFRKLPIQKVGIRHIRYPITVRDKSHEKQ
                     NTVASVTFSVNLPHSDRGTHMSRFVEVLDRFKNEVSYHTLDAILVETARTLQAEEAHI
                     ELAFPFFLRKQAPISGLESTMSYDCVIYADYRDRLEIATRVEVPVHTLCPCSKEISQY
                     SAHNQRGTVVITVGMTRFIWIEDLIEVAEDAASSPLYALLKREDEKFVTERAYENPRF
                     VEDVVREVALRLDRLPGLRFYTIEVENFESIHNHSAYAVISKRFDEE"
     gene            1897..2709
                     /locus_tag="VFH83_12220"
     CDS             1897..2709
                     /locus_tag="VFH83_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487182.1"
                     /translation="MKSRSGLLAPPMTRRTRGFRLYDFQGHRLIDLHRECAFLGHRGA
                     PITAMKSALSQGLDSGLPSVWEGRLIRALGRRFPAHRTVALYASVDRALAALTQALGR
                     RVTPQDLYDPALDPAPEREPPAALWRPLLPTPSSRVLLLVMPLTVCGAPAPLCVDDPE
                     AELPPSDPISGFVLAGAHRAFLSLWPSDGSAPLRDPALERIVEGSPGWELRGPYVKTL
                     SSPEEYQGILRRFLEAGVLLCPRYPGPSVLPAGCSPGERNLLAKLFASCPGG"
     gene            2712..3011
                     /locus_tag="VFH83_12225"
     CDS             2712..3011
                     /locus_tag="VFH83_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487183.1"
                     /translation="MDITAFAGQTTHLVVGAFATFSAILLWSRTRDMAWTFLIIGVII
                     AYADIVFSTLGAFGIVVEDPLVYNGVPLLRLALANFPLLFSGVGFLVAAARRHGS"
     gene            3014..>3350
                     /locus_tag="VFH83_12230"
     CDS             3014..>3350
                     /locus_tag="VFH83_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487184.1"
                     /translation="MPSIHLIPARDESNIEQKLVTLILAAVTQKPDLILSVFAGTPAF
                     GVYRLLVNRARLEVVDFSRVRFVVFDELIFPGSGTAARPEGAAPFRTVLEERLFTPLS
                     IPPDNIVAYN"
BASE COUNT          522 a         1131 c         1102 g          595 t
ORIGIN      
        1 cgggctgctc caggatgggt tcccgggcac gaccgccatc ctctaccgca tgaacgcgca
       61 gtcccgacac ttcgaagacc ggttccgggt ggaagggatc ccctaccggc tcatcggcac
      121 ggtccggttc tactcccgcc aggaggtcaa ggacgcgctg gcctaccttt ccctgctggt
      181 gaaccctcgg gacgaggtgg catttcgccg gatcgccaac accccgagcc gagggctcgg
      241 cccggccagc gtggaaaaga tcgtggccct gcggggtcct ggagagtccg tggaagacgc
      301 atgccgccgg gcccaggtgc ctcctcgcgc tcgcctcggc gtacgggagc tcctgggctg
      361 tctgcaggaa tgcgccgccc gactggagga gctgccgctc tccgagcttg cccggctgct
      421 gctcacccgg tcggggctgt acgagcacta ccgccagcgg gatcggtccg acgggacctc
      481 caagagcgcg aatctggaag agatggtcag cgccatggcg ccctatgggg cgggcgcgga
      541 agcgctcggg gccttcctgg aaagcgtcgc gctcgccaac ccggcggatg aggccgagca
      601 gtccggagaa gagccgaagg tgaccctcat cacccttcac aacaccaagg ggctggagtt
      661 cgaccgggtg atcatcagcg ggctggagga aggcatcttt ccgcacgacg ccagctcgtt
      721 gagcgcggag gacctggaag aggagcgccg gctgttctac gtcggcatca cgcgggcgcg
      781 ggagcggctc accatgacgt ggtgccttcg gcgcaggatt ttcggcaagg tcatcgagaa
      841 tcctccctcc cggttcctgg acgagatccc gaaggagcag atcaaccgcg tggagagcga
      901 ggccgccgcg ttcctgttgg gacggggagc cggctccgag gcggacgact atccgccggg
      961 tgcgggggtg ttccacgacg agtacgggcc cggcgtcgtg gaacgaacat ggtatactga
     1021 cggctcgctg ctggtgcagg ttcgattcca atcggggagg gtagcgaagt tcctcccccg
     1081 gtacacgcga ttggagcgca taggctttga tgagtaacga ccgaacgcag ctgaaggaca
     1141 ttcagggtgg acccgacttc cgaaagcttc cgatccagaa ggtcgggatc cgccacatcc
     1201 gatatccgat caccgtccgc gacaagagcc acgagaagca gaacacggtc gccagcgtga
     1261 ccttctcggt gaacctgccc cacagcgatc ggggcaccca catgagccgg ttcgtggagg
     1321 tcctggatcg gttcaagaac gaggtcagct accacaccct ggacgccatc ctggtggaga
     1381 ccgcgcgcac gctccaggcg gaggaagccc acatcgagct cgccttcccc ttcttcctgc
     1441 ggaagcaggc gcccatctcc ggcctggaat ccacgatgtc gtacgactgc gtgatctacg
     1501 cggactaccg ggaccgcctg gagatcgcca cgcgcgtcga ggtgcccgtg cacacgctct
     1561 gcccgtgctc caaggagatc tcgcagtatt ccgcgcacaa ccagcgtggg accgtggtga
     1621 tcaccgtggg catgacgcgg ttcatctgga tcgaggacct catcgaggtc gccgaggatg
     1681 cggcttccag ccccctgtat gccctgctca aacgagagga tgagaagttc gtgacggagc
     1741 gcgcctacga gaacccgcgg ttcgtggagg acgtggtgcg ggaggtggcc ttgaggctcg
     1801 atcggctgcc ggggctccgg ttctacacga tcgaggtgga gaacttcgag tccatccaca
     1861 accacagcgc ctatgccgtc atcagcaagc gcttcgatga agagtaggag cggcctcctc
     1921 gctcctccga tgacccgccg gacccggggt ttccgtctct acgacttcca gggacaccgc
     1981 ctcatcgatc tccaccgcga atgtgctttc ctggggcacc ggggcgcgcc gatcaccgcc
     2041 atgaagtccg cgctgtccca gggcctggat tcgggccttc cgtccgtgtg ggagggtcgg
     2101 ctgatccgag cgctggggcg ccggtttccg gcgcaccgca ccgtggcgct gtacgcctcg
     2161 gtggaccgcg ccctggccgc gctgacccag gcgctgggcc gccgggtcac gccgcaggac
     2221 ctgtatgatc cggcgctgga ccctgcgccg gagcgcgagc cgccggcggc gctctggaga
     2281 ccccttctgc cgacgccctc gtcccgggtc ctgctcctgg tgatgccact gaccgtttgc
     2341 ggcgctccgg ctcccctgtg cgtggatgac ccggaggcgg agctgccgcc gtcggatccc
     2401 atcagcggat tcgtccttgc cggcgcgcac cgcgcgtttc tgtccctctg gccctcggac
     2461 ggctccgcgc cccttcggga tcccgcgctg gagcgcatcg tcgaaggatc gccgggctgg
     2521 gagcttcggg ggccctacgt gaagaccctg agctcgcccg aagagtacca ggggatcctg
     2581 cgtcggttcc tggaagccgg ggtcctccta tgtccgcgtt accccggacc gagcgttctg
     2641 cccgcgggct gctccccggg cgaacgcaac ctcctggcga agcttttcgc cagctgcccc
     2701 ggaggctgag gatggacatc acggcgttcg ccggtcagac gacccacctg gtggttggcg
     2761 ccttcgccac gttctccgcg atcctgctgt ggtcccggac ccgcgacatg gcctggacct
     2821 tcctgatcat cggggttatc atcgcttacg ctgatatcgt gttctcgacg ctgggtgcgt
     2881 tcgggatcgt cgtggaggac cccctggtgt acaacggcgt tccgctcctg cggctcgccc
     2941 tggcgaactt ccccttgctc ttttcgggcg ttggtttcct ggtggcagcc gctcgccgcc
     3001 acgggtcctg agcatgcctt ccatccatct catcccggcg cgggatgaat cgaacatcga
     3061 gcagaagctc gtcaccctta tcctggccgc ggtcacccag aagcctgatc tcatcctgtc
     3121 cgtgttcgcc gggacgccgg ccttcggcgt gtaccgcctc ctggtgaatc gagcgcggct
     3181 ggaggtcgtg gacttctccc gggtgcggtt cgtcgtgttc gacgagctca tcttcccggg
     3241 aagcggtacg gcggcccgcc ctgagggcgc ggccccgttt cggaccgtgc tggaggagcg
     3301 gctcttcacc ccgctctcca tacctccgga caatatcgtt gcctacaacc
//
LOCUS       DASRZS010000499         2998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2968691, whole genome shotgun sequence.
ACCESSION   DASRZS010000499 DASRZS010000000
VERSION     DASRZS010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2968691"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VFH83_12235"
     CDS             <1..510
                     /locus_tag="VFH83_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HET6487185.1"
                     /translation="DSFYRVGALVFGGGHVVLPLLQREVVPAGLTTNPVFLAGYGAAQ
                     AVPGPLFSFAAYLGAVIAPRPIAGAAIALCAIYLPSFLLLGGVLPYWDYLRSQRAVRS
                     ALTGINAAVVGILLAALYSPVWTSAITGPVDMAMALVAFLLLALWKVRPWMIVLGGAA
                     IGELLSLLR"
     gene            complement(553..1503)
                     /locus_tag="VFH83_12240"
     CDS             complement(553..1503)
                     /locus_tag="VFH83_12240"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON domain-containing protein"
                     /protein_id="HET6487186.1"
                     /translation="MRAIANLFYVVLGALGYGHPFHPVLTHLVVGPVLVAFLLGLIAW
                     IFRRPVLHQTARHLTVFSLVAWFFTVAVGILDWVHFYGADLGIFEIRMKLILASALLV
                     VLIGTLVLHRRVPKESSAPVIAYAVSALLVVLLGYYGGNLVYASKTASSESTSSSAAQ
                     GQTLLKDGYTEKVVNGYTLDWKVDGKLLHVRMSHGSAGWLGVGFGHTGTMEGSDIKIG
                     YLANGKPVIVDSFGDAPNHHSAITDLGGTSDVTDVSGSLQGSVTSFSFTVPLAPGDKW
                     HAAFASGTSYDVIMAAGPDGAEDDSSYHGPGNYAVFKIVL"
     gene            1639..2826
                     /locus_tag="VFH83_12245"
     CDS             1639..2826
                     /locus_tag="VFH83_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HET6487187.1"
                     /translation="MRKPVSANVSWVGKVDWELRKFHGDEYSTWRGSSYNAFVIEEKK
                     VALIDTVWQPYANEFVDALERDVGLEKLDFIVSNHGEVDHSGSLAELLRRRPGLPVYC
                     TANGVKSLRGQYHQDWDFHTVKTGDRLSLGDKELVFIEAPLLHWPDTMMCYLTGDQIL
                     FSSDAFGQHYARERLFNDLVDPVELFEEAIKYYANILTPFSALVTRKIDELLALNLPV
                     DMICPSHGVIWRQDPMQIVRKYAEWAKDYQEDRVVIAYDTMWEGTRHLAEAVAQGIGE
                     ASPSTAVVLHNCAKSDKNDVVTDVFRSRAVLFGSPTVNRGILTSVAGLFEMLRGLKLK
                     GKKAGAFGCYGWSGESVALLTTAAKEAGFQVLNDGMKALWDPDESALARAVEYGREFA
                     RSL"
BASE COUNT          527 a          972 c         1003 g          496 t
ORIGIN      
        1 gacagcttct accgcgtggg ggcgctcgta ttcggaggcg gccacgtggt gctcccgctt
       61 ctgcagcggg aagtcgtccc ggccggcctc accaccaacc cggtattcct tgccggttac
      121 ggggccgccc aggccgtgcc cggccccctc ttttccttcg cggcgtacct gggcgccgtg
      181 attgcgccgc ggccgattgc cggcgcggcc atcgcgctct gcgccatcta tctgccgtcc
      241 ttcctgctcc tgggcggcgt gctgccctac tgggactacc tgcggagcca gagagcggtt
      301 cgctcggcgc tgacagggat caacgccgcg gtggtcggca tcctgctggc cgcgctgtac
      361 agcccggtct ggacgagtgc catcacggga cccgtggata tggcaatggc gcttgtcgcc
      421 tttctcctgc tggccctctg gaaggtccgg ccgtggatga tcgtgctggg cggagccgcc
      481 ataggagagc tcctcagcct cctccgttga ccgcgtgcga ccccagaagc gcccgcgctc
      541 cacggagcgg cgtcagagca cgatcttgaa gacggcgtag ttcccggggc cgtggtacga
      601 ggagtcgtcc tcggcgccgt ccggtcccgc cgccatgatg acgtcatagg aggtgccgga
      661 ggcgaacgcg gcgtgccact tgtctcccgg agcgagcggg accgtgaagg agaacgaggt
      721 gacggagccc tggagggacc cgctcacgtc ggtcacatcg gacgttccgc cgaggtccgt
      781 gatggcgctg tggtggttgg gagcatcgcc aaaggagtcc acgatcaccg gcttgccgtt
      841 ggcgaggtag ccgatcttga tgtcggagcc ctccatggtc cccgtgtggc cgaagcctac
      901 gcccagccag cccgcgctgc cgtggctcat gcggacgtgg agcagcttgc cgtccacctt
      961 ccagtccagc gtgtagccgt tgaccacctt ctccgtgtag ccgtccttca gcagggtctg
     1021 cccctgggcg gcgctggatg acgtcgactc ggaggaggcc gtcttagagg cgtagaccag
     1081 gttcccgccg taatacccca gaagcacaac caggagagcc gacaccgcat aggcgattac
     1141 gggcgcggaa gactccttgg gcacgcggcg gtggagcacc agggtcccga tcagcaccac
     1201 gaggagggcc gaagccagga tgagcttcat ccggatctcg aagatcccga ggtccgcgcc
     1261 gtagaaatgg acccagtcca ggatcccgac ggccaccgtg aagaaccacg ccaccagcga
     1321 gaacaccgtg aggtggcgcg cggtctgatg cagcaccggc ctgcggaaga tccacgcgat
     1381 caacccgaga aggaaggcaa ccagcaccgg acctaccacg aggtgggtga gcacgggatg
     1441 gaagggatgg ccgtaaccga gcgctccgag aacgacgtag aagaggttgg ctatcgcgcg
     1501 catgacagac aacctatcca ccggcgtctc cgatcgtcaa ggaaaaccgc cggctcggct
     1561 tgcgcccggc cccccttcgc tcgatgggcc gatccggtgt aaccttgtcc tatctcacct
     1621 gcaaggagcg aatggctcat gaggaagccg gtttcggcga acgtgtcctg ggtcggcaag
     1681 gtcgactggg agcttcggaa gtttcacggt gacgaatact ccacctggcg aggatcgagc
     1741 tacaacgcgt tcgtcattga agagaagaaa gtcgcgctca tcgacaccgt ttggcagccc
     1801 tacgcgaacg aattcgtgga tgccctggag cgggacgtcg gcctcgagaa gctcgacttc
     1861 atcgtctcca atcatggcga ggtcgaccac tcgggatccc tggccgagct cttgaggcgg
     1921 cgcccggggc tccccgtgta ctgcaccgcg aacggagtga agtccctgcg gggccagtat
     1981 caccaggact gggacttcca caccgtcaag acgggagacc ggctgagcct gggagacaag
     2041 gagctcgtct tcatcgaggc tcccctcctg cattggccgg acaccatgat gtgctatctc
     2101 accggagacc agatcctctt ctccagcgac gctttcggcc agcactatgc ccgggagcgc
     2161 ctcttcaacg acctggtgga tccggtggag ctcttcgaag aggccatcaa gtactacgcc
     2221 aacatcctca cgcccttcag cgcgctggtc acccggaaga tcgacgagct cctggcgctc
     2281 aaccttccgg tggacatgat ctgtccgagc cacggcgtga tctggaggca ggacccgatg
     2341 cagatcgtgc ggaagtacgc ggagtgggcc aaggactacc aggaggaccg ggtcgtcatc
     2401 gcctatgaca cgatgtggga agggacgcgc catctggcgg aggcggttgc gcaggggatc
     2461 ggcgaggcat ctccgtccac cgccgttgtg ctccacaact gcgccaagag cgacaagaac
     2521 gatgtcgtca cggacgtctt tcggtccagg gcggtcctgt tcgggtcgcc gacggtgaac
     2581 cgcggcatcc tcacctccgt ggcggggctg ttcgagatgc tgcgcgggct caagctcaag
     2641 ggcaagaaag cgggcgcgtt cggctgctac ggatggtccg gggagtccgt tgccctgctg
     2701 accacggcgg ccaaggaggc cggattccag gtgctgaacg acgggatgaa ggctttgtgg
     2761 gatcccgatg agtcggcgct ggccagggcc gtggagtacg gccgggagtt cgcccgctcg
     2821 ctgtaggccg ctcgcgggag gccggggccg cgcgccgggg cacgccgcgc ggcctccccc
     2881 ggccagccat cgagcccgct caggcgcccg cgaaccccac gatcaggtcc gtaaagtacc
     2941 cgaggaagag gcccaccacc accgcggtca gaaggggcag cttctgcagc gtgtgcct
//
LOCUS       DASRZS010000500         3670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5277327, whole genome shotgun sequence.
ACCESSION   DASRZS010000500 DASRZS010000000
VERSION     DASRZS010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5277327"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VFH83_12250"
     CDS             <1..764
                     /locus_tag="VFH83_12250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6487188.1"
                     /translation="RIASLTRSFDHMRRRVQEETATRARFIMAVSHDLKTPLSSISGY
                     LDAIRDGIASEPAQLDKYLAIIRDKASLLESRIGQLIDYVKLDTADWRRSRESIPFAE
                     FLNEAVIVFSTEAEARGFGFDAVVDIPPAATVYMDGDLVYRALENLVQNAFRYGEPQS
                     TIRFEAGTRDGLTTLRISNRGQAIPEEDLPFIFEPFFRGTRARREGGFGLGLSVVKFV
                     VSSHGWDIEAVSADGETCFTLSIPATAVPAARAPT"
     gene            851..1549
                     /locus_tag="VFH83_12255"
     CDS             851..1549
                     /locus_tag="VFH83_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487189.1"
                     /translation="MTIVELKDAHKTYLLGKVQVEAVRGVSFAIEKGDFVSIAGPSGS
                     GKSTILNLIGCIDTPTSGAVEIEGKPTAGLSDAALTRLRHETLGFIFQSFNLIPVLDV
                     YENIEFPLLLGNGKRSKAERRDWVDSLIVEVGLEQWRRHKPNELSGGQRQRVAIARAL
                     VTRPAIVLADEPTANLDSKTGEQIISLMKKINRDLSTTFIFSTHDAHIVGIADHVIRL
                     SDGTVIENTRGTEA"
     gene            1554..3005
                     /locus_tag="VFH83_12260"
     CDS             1554..3005
                     /locus_tag="VFH83_12260"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6487190.1"
                     /translation="MLVLIRIALRNLREHTSKSLIIGTLIVLGVIIIVVGNSLLDTAE
                     RGVHRMFIDNYTGDVFLSGVPRTPGAKVSLFGLQAAGDSESTPILPEYDTVVAHLKAD
                     PRVDGVTSQLTGVSLVSVEGNDHNTVALLFGVDPETYPRMFHDTFLVQGSYLKPGQEG
                     IVVSRGWLDDAEKNLKVRLKVGDKVILNGFGRAGFKIREATIVGVVDFNAESQGMSVI
                     AWANADTVRILSGVDVTAEDVVLSKDETALLSAGSENDIFSASESTVSAAQANAPVVT
                     VKEKAPPTPSTDGGSWHFLLARLKDSGQTDRFIRDTNAWLASQGIDARAGGWRDAAGP
                     FAQSIDVVRTVFSVAILIVAIVAVIIIMNTLVISVIERTGEIGTMRALGAQRPFVWLM
                     FLVETLTVTIVFGLVGIVLASGLIGLVNLFHLQTSSDFIQILFGGKVLRMAVRPVSFL
                     TTLAMVALVGLVSHLYPVIVALRVPPVRAMQGE"
     gene            3015..>3670
                     /locus_tag="VFH83_12265"
     CDS             3015..>3670
                     /locus_tag="VFH83_12265"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487191.1"
                     /translation="MALASIALKNLNRQKKRSFLLGGAIAFGVLIVTVIDGFSGAFMQ
                     NVSRNFADIAAGHIFVEGAEKTASGKTVSVIRDDSVLMDVLKKTGVPAQYISRRSIGS
                     GTLIFESRKTQIGIRGVDFSRESYLKDRLTLSQGSFKDMENRQGLILSESTAKRLNAQ
                     IGDRLLFQLTTVTGQQNVGELVLVATTPDNGLMSGFSAYANLSYLNELLNLGPNEYQT
                     "
BASE COUNT          654 a         1197 c         1187 g          632 t
ORIGIN      
        1 accgcatcgc ctccctcacg cggtctttcg accacatgcg tcggcgcgtc caggaggaga
       61 cggccacgcg cgcccggttc atcatggccg tctcccatga cctgaagacc cccctctcca
      121 gcatctccgg ctacctggac gccatccggg acgggatcgc gtcggagccc gcgcagctcg
      181 acaagtacct ggccatcatc cgggacaagg caagcctcct ggaaagccgc atcggccagc
      241 tcatcgacta cgtaaagctg gacaccgcgg actggaggcg ctcgcgggag agcatcccct
      301 tcgcggagtt tctcaacgag gcggtcatcg tcttcagcac cgaggcggaa gcgcgcgggt
      361 tcgggttcga tgccgtggtg gatatcccgc cggccgccac cgtctacatg gacggagacc
      421 tggtgtatcg ggcgctggag aacctggtgc agaacgcctt ccgatatggc gagccccaat
      481 cgaccatacg gtttgaggcc gggacccggg atggcctgac aacgctgcgg atctcgaacc
      541 ggggccaggc gattcccgag gaggatcttc cgttcatctt cgagccgttc tttcggggga
      601 cccgcgcccg acgggaaggc gggttcgggc tgggtctgag cgtcgtgaag ttcgtcgtct
      661 ccagccacgg gtgggacatc gaggcggtct ccgcggacgg ggagacctgc ttcacgctgt
      721 ccatcccggc gacggccgtc ccggccgccc gcgcacccac ataacacctt gtaagaattc
      781 ttcattcctc ggatatggtc atttccgggc gcatcggtat gttgtccgac gagagacacg
      841 ggagaggcaa atgacgattg tcgagctgaa agacgcgcac aagacgtacc ttctgggcaa
      901 agtccaggtc gaggcggttc ggggggtgag ctttgccatc gagaagggag acttcgtctc
      961 cattgccggc ccctcggggt ccggcaagtc cacgatcctg aacctgatcg gttgcatcga
     1021 tacccccact tcgggggccg tggagatcga ggggaagccc accgcgggcc tctccgatgc
     1081 cgcgctcacc cgactgaggc acgagaccct ggggttcatc ttccagagct tcaacctcat
     1141 accggttctc gacgtgtacg agaacatcga gttccccctc ctcctcggca acgggaagcg
     1201 gtccaaggcg gagcggcgcg actgggtgga ctccctcatc gtcgaggtgg gtctggagca
     1261 gtggcggcgg cacaagccca acgagctttc cggcggccaa cgccagcggg tggccattgc
     1321 ccgagcgctg gtgacccggc cggccatcgt gcttgcggac gagcccacgg ccaacctgga
     1381 ctccaagacc ggggagcaga tcatctccct catgaagaag atcaaccgcg acctgtcgac
     1441 gacgttcatc ttttccaccc acgacgcgca catcgtcggg atcgcggacc acgtcatccg
     1501 gctgagcgac ggaaccgtga tcgagaacac ccgcgggacg gaggcgtgag cccatgctcg
     1561 tcctgatccg catcgcgctt cggaaccttc gggagcatac ctccaagagc ctcatcatcg
     1621 gcaccctcat cgtgctcggc gtcatcatca tcgtggtggg caacagcctc ctggacaccg
     1681 cggagcgggg cgtgcatcgc atgttcatcg acaactacac cggggacgtc ttcctctccg
     1741 gtgtccccag gaccccgggc gccaaggtga gcctcttcgg tctgcaggcg gccggggact
     1801 cggagtccac gccgatcctg ccggagtacg acacggtggt ggcgcacctg aaggcggatc
     1861 cccgtgtgga cggcgtcacc agccagctca ccggagtgag cctggtgtcc gtggagggga
     1921 acgaccacaa caccgtggcg ctcctcttcg gggtggatcc cgagacgtac ccccgaatgt
     1981 tccacgacac cttcctggtg cagggcagct acctgaagcc gggccaggaa gggatcgtcg
     2041 tatcccgggg atggctcgac gacgcggaga agaacctcaa ggtgcgcctc aaggtcgggg
     2101 acaaggtgat cctcaacggc ttcggccgcg ccggtttcaa gatccgcgag gccacgatcg
     2161 tcggtgtggt ggacttcaac gcggagagcc aggggatgag cgtgatcgcc tgggccaacg
     2221 cggacacggt tcgcatcctg agcggggtgg acgtcaccgc ggaggacgtc gtgctctcca
     2281 aggacgagac ggcgcttctg agcgccggca gcgagaacga catcttctcc gcgtccgaaa
     2341 gcacggtcag cgccgcgcag gccaacgctc cggtggtcac ggtcaaggag aaggctcccc
     2401 ccacgccgtc gaccgacggt ggctcctggc acttcctgct ggcgcggctc aaggactccg
     2461 gtcagaccga ccgcttcatc cgggacacca acgcctggct cgcctcccag ggcatcgacg
     2521 caagggccgg gggatggcgg gacgccgcgg gtccgttcgc tcagagcatc gacgtggttc
     2581 ggaccgtgtt ctccgtggcc atcctgatcg tggccatcgt ggccgtgatc atcatcatga
     2641 acacgctggt catctccgtc atcgagcgaa cgggggaaat cgggacgatg cgcgccctgg
     2701 gggcgcagcg gccgttcgta tggctgatgt tcctggtgga gaccctgacc gtcaccatcg
     2761 tgttcgggct cgtgggcatt gtcctggcat ccgggctgat cgggctggtc aacctgttcc
     2821 acctccagac ctcgagcgac ttcatccaga tcctcttcgg aggcaaggtg ctgaggatgg
     2881 cggtccggcc ggtgtccttc ctgaccacgc tggccatggt ggcgctggtg gggctggtct
     2941 cccacctgta tccggtgatc gtcgcgctca gggtcccgcc ggtgcgggcc atgcagggcg
     3001 agtaggagag acccatggcg cttgcatcca tcgccctcaa gaatctcaac cggcagaaga
     3061 agcggagctt cctcctgggt ggggccatcg ccttcggcgt gctgatcgtg accgtgatcg
     3121 acgggttctc cggcgcgttc atgcagaacg tgagccgcaa cttcgcggac atcgccgccg
     3181 gccacatctt cgtggagggc gcggagaaga ccgcctccgg gaagaccgtc tccgtgatcc
     3241 gcgacgactc ggtcctgatg gatgtattga agaagaccgg ggtcccggcc cagtacatct
     3301 cccgacgctc catcggctcc gggacgctta tcttcgagag ccgcaagacc cagatcggga
     3361 tccgcggggt ggacttctcc cgggagagct acctcaagga ccggctgacc ctttcccagg
     3421 gaagcttcaa ggacatggag aaccgccagg gcctgatcct gagcgagtcc acggccaaga
     3481 ggctgaacgc gcagatcggg gaccgtcttc tgttccagct caccacggtc acgggtcagc
     3541 agaacgtggg agagctggtc ctggtggcaa ccactccgga caacgggctg atgtcggggt
     3601 tctccgcgta cgccaacctc tcctatctga acgagctcct caacctgggg cccaacgagt
     3661 accagacctt
//
LOCUS       DASRZS010000501         3978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4895523, whole genome shotgun sequence.
ACCESSION   DASRZS010000501 DASRZS010000000
VERSION     DASRZS010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4895523"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFH83_12270"
     CDS             <1..210
                     /locus_tag="VFH83_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008046014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6487192.1"
                     /translation="GFLGSFQSFDAIYVLTQGGPMMSTRVIVFYLWQNAFSFYKMGFA
                     SAQAWFLFVILIGLTYVQFRVYRER"
     gene            216..1061
                     /locus_tag="VFH83_12275"
     CDS             216..1061
                     /locus_tag="VFH83_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6487193.1"
                     /translation="MKASGYVVRRSLAHGVLYVIVVLTLITMVFPLLWMISTSFKTYQ
                     TALTFPPRMIPEFLNWQSYTHVLQTSHLPRYFLNTALYSIVGTILEVFFAILGGFVFA
                     KYRFPGSGAFFSVILATIMIPGQVTLIPVFLILRDFHWLDTFWALIIPGIASPFGIFL
                     IRQFGRGIPDDLIEAARIDGYPEFRILFQIFVPLCMPAITTLAVLAFMGRWNDLFWPL
                     IVTTSEYMGTLQLMLTKISRSLYDIVWNELMAGMTMACIPIVIIYIAFQRYFTQGIVL
                     TGIKG"
     gene            1193..3847
                     /locus_tag="VFH83_12280"
     CDS             1193..3847
                     /locus_tag="VFH83_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HET6487194.1"
                     /translation="MAAVQKEIHFTMGTHLDLFWMGAPRDCLDRGTEIIVEALELCLK
                     HKEYCYYIESVVFAEHLLKLHPEKKPLMAELIKSGNLEIGGAYADRVEHSHEGESILR
                     HHIYAVEWIQKTFGITPRSSCHSDLPGLSPQVPQILAKCGIRYYIRARGVCAVYTWEA
                     PDGSSILYGNFGSNYGEKTMPEMDEELKDAGGFPPKVIMRGGYGDLQMVNDHILALIQ
                     DLKRKYPQYGFQMSSPSDVLDFYRENESARRSLPRIKGEWPYGWGNGGSLLVEDYRLE
                     MALENALLAGEKAVTAARLLGHALRPKTDRAMWWCSLFRYAREGEPPVIEKGAELQEA
                     WKAEMFCQDHNYSGFSGNKSDHDRSVMLKYALAYIGGITDNALSDVCGSIGTPSFPHA
                     DRAAARVVVFNPLSWPRDEVVRVTLPGPLVDAPILLVDEKGSAQGFERSKGELSFTAK
                     AVPSVGYRPYFVLTIPENEIHRLEPPSPIHVSQSFDLHDWHREFVRWQNSLPEYREPW
                     LAETPEGDGLRLETAHYRIHLSGKHGYIGGIYSKKLGRELTGDRASRRFAELLSYEDP
                     GIDVRYCFTGSYTRDSQSSFALRKVSSDSVSATFALEGYFNLARVEKRITLYAESPAI
                     DLEVVIYWWNDHGSHVRLCLPFSEKDFRQTWYGVPYYAMQWPLMMEGVADSVILGMEK
                     KPDELRYEDRRHFREVVKWVDVGYADCGVTLAVQLPNMWIDDSLLEVPLIRSNRSCGD
                     HNVWADNSGRQVWKFRLNPHAGDWRSGKAYRTGWDLNNPLAARVTGPSASGALRAEPA
                     SFFRVSQDNVVLSVAKPANDGSGDVILRYCEMEGKKGTVEIECLKTPRTAHEADLLER
                     PGAKLTVNGNRILAPTAPYEIKTIRCSI"
BASE COUNT          704 a         1286 c         1264 g          724 t
ORIGIN      
        1 gggttcctgg gatcgttcca gagcttcgac gccatctacg tgctcaccca gggcgggccg
       61 atgatgtcca cccgggtgat cgtcttctac ctctggcaga acgcgttctc cttctacaag
      121 atggggttcg cctccgccca ggcctggttc ctcttcgtga tcctgatcgg cctgacctac
      181 gtccagttcc gcgtgtaccg ggagaggtga gcaagatgaa ggcgtccggc tacgtagtcc
      241 gtcgttccct cgcgcacggg gtgctgtacg tgatcgtggt cctcaccctg atcaccatgg
      301 tctttccgct cctgtggatg atctcgacgt ccttcaagac gtatcagacc gccctgacct
      361 tccctccgcg gatgatcccg gagttcctga actggcagtc ctacacccac gtgctccaga
      421 ccagccatct gcctcggtac ttcctgaaca ccgccctgta ctccatcgtg gggaccatcc
      481 tcgaagtgtt tttcgccatc ctggggggat tcgtgttcgc caagtaccgg ttccccggga
      541 gcggcgcttt cttcagcgtg atcctggcca ccatcatgat cccggggcag gtcaccctga
      601 tccccgtctt cctgatcttg agggacttcc actggctgga taccttctgg gcgctcatca
      661 ttcccggcat cgccagcccg ttcgggatct tcctcatccg gcagtttggc cgcggaatcc
      721 cggatgacct gatcgaggcg gcgcggatcg acgggtatcc cgagttccgc atcctcttcc
      781 agatcttcgt ccccctgtgc atgccggcca tcaccaccct cgcggtcctg gcgttcatgg
      841 ggcggtggaa cgacctgttc tggcccctca tcgtcaccac cagcgagtac atggggaccc
      901 tccagctcat gctgaccaag atcagccgga gcctctatga catcgtctgg aacgagctta
      961 tggcgggaat gaccatggcc tgcatcccga tcgtcatcat ctacatcgcc ttccagaggt
     1021 acttcacgca gggcatcgtg cttaccggaa tcaagggata gtcccggatc gcatcgccgc
     1081 cccggagcgc cgccccggag cgccgcgcgc cgcgaggcgg cgatggtatc gccaacgatt
     1141 tgtggcccgg ctgggttgga gcccgaggcc ggccaccgaa aggagcgtgt tcatggccgc
     1201 ggttcagaag gaaatccact tcaccatggg gacccacctc gacctgttct ggatgggcgc
     1261 cccgcgggat tgcctcgacc ggggaacgga gatcatcgtc gaagcgctgg agctctgtct
     1321 caagcacaag gagtactgct actacatcga gtcggtcgtg ttcgcggagc atctgctgaa
     1381 gctccatccg gagaagaagc ccctcatggc ggagctcatc aagtccggga acctggagat
     1441 cggcggcgcg tacgcggacc gggtcgagca ctcccacgag ggggagtcca tcctgcgcca
     1501 ccacatctac gcggtggagt ggatccagaa gaccttcggg atcacgccgc ggagctcctg
     1561 ccattccgac cttccgggac tgtccccgca ggtcccacag atcctggcca agtgcggcat
     1621 ccgctactac atccgcgccc ggggcgtctg cgccgtgtac acctgggagg cgccggacgg
     1681 ctcctccatc ctctatggca acttcggctc caactacggc gagaagacca tgccggagat
     1741 ggacgaggag ctcaaggacg ccggcgggtt cccgcccaag gtgatcatgc ggggcgggta
     1801 cggcgacctc cagatggtga acgaccacat cctggccctc atccaggatc tgaagaggaa
     1861 gtacccgcag tacggcttcc agatgtcttc tccctccgac gtgctggact tctatcggga
     1921 gaacgagagc gcgcgccgat ccctgcctcg gatcaagggc gagtggccct acggctgggg
     1981 caacggtgga agcctcctgg tggaggacta ccggctggag atggcgctgg agaacgcgct
     2041 tctggcgggg gagaaggccg tgacggccgc tcgcctcctg gggcacgcgc tacggcccaa
     2101 gacggaccgc gccatgtggt ggtgcagcct cttccgctac gcccgggagg gcgagccgcc
     2161 cgtcatcgag aagggcgccg agctgcaaga ggcgtggaag gcggagatgt tctgccagga
     2221 ccacaactac agcggcttca gcggcaacaa gagcgatcac gatcggtccg tgatgctgaa
     2281 gtacgcgctg gcgtacattg ggggcatcac cgacaacgct ctctccgatg tctgcggttc
     2341 catcgggacc ccgtccttcc cgcacgcgga ccgcgctgcg gcgcgggtgg tggtcttcaa
     2401 cccgctctcc tggccgaggg acgaggtggt gcgcgtcacg cttcccgggc cgctcgtgga
     2461 cgcgccgatc ctcctggtgg acgagaaagg ctcggctcag ggcttcgagc gctccaaagg
     2521 cgagctctcc ttcaccgcca aggcggttcc gtccgtggga tatcggccgt acttcgtgct
     2581 cacgatccct gagaacgaga tccatcgcct ggagccgccc tctcccatcc acgtcagtca
     2641 gagcttcgac ttgcacgact ggcaccggga gttcgtccgc tggcagaaca gcctgccgga
     2701 gtatcgcgag ccgtggctcg cggagacacc cgaaggagac ggcttgcgcc tggagacggc
     2761 gcactaccgg atccacctct cggggaagca cgggtacatc ggcggcatct actcgaagaa
     2821 gctgggccgc gagctcacgg gagaccgcgc ttcgcgccgc ttcgccgagc ttctctccta
     2881 cgaggacccg ggtatcgacg tgcgttactg cttcaccggg agctataccc gggacagcca
     2941 gtcctcgttc gcccttcgca aggtctcttc ggactccgtc tcggcgacct tcgccctgga
     3001 ggggtatttc aacctggccc gcgtggagaa gcgcatcacc ctgtacgccg aatcaccggc
     3061 catcgacctg gaggtcgtga tctactggtg gaacgaccac gggtctcacg tccgtctgtg
     3121 cctgccgttc tccgagaagg acttccgcca gacctggtac ggggttccct actacgccat
     3181 gcagtggccc ctgatgatgg aaggggtcgc ggactccgtc atcctcggga tggaaaagaa
     3241 accggacgag cttcgctacg aggaccgtcg ccacttccgc gaggtggtga agtgggtgga
     3301 cgtggggtac gccgactgcg gggtgacgct tgccgtccag ctccccaaca tgtggatcga
     3361 cgacagcctc ctggaggtgc ccctgatccg cagcaaccga agctgcggag accacaacgt
     3421 ctgggccgac aactcggggc ggcaggtctg gaagttccgc ctcaatcctc acgcggggga
     3481 ctggcggtcg ggcaaggcct accgcaccgg ttgggacctc aacaatccac tggccgctcg
     3541 ggtcacgggg ccatcggcct ccggggccct tcgggcggag cccgcgtcct tcttccgggt
     3601 ctcgcaggac aacgtcgtcc tcagcgtggc caagccggca aacgacggga gcggcgacgt
     3661 gatccttcgc tactgcgaaa tggaggggaa gaaggggacc gtggagatcg agtgcctcaa
     3721 aacccccagg accgcgcacg aggcggacct gttggagagg ccgggcgcga agctgacggt
     3781 caatggaaac aggatcctgg cgccgacggc gccctatgag atcaagacga ttcggtgctc
     3841 gatctgagga ggaagagatg agcgagatgg gtctggttac caggctggta gggaagaaca
     3901 aggtagagat cgtggagaag ccgcttccgg aggtcaagga agacggcatg ctggtggagg
     3961 taggcctcgc cgggatct
//
LOCUS       DASRZS010000502         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1815674, whole genome shotgun sequence.
ACCESSION   DASRZS010000502 DASRZS010000000
VERSION     DASRZS010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815674"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VFH83_12285"
     CDS             complement(<1..544)
                     /locus_tag="VFH83_12285"
                     /inference="COORDINATES: protein motif:HMM:NF042553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6657 family protein"
                     /protein_id="HET6487195.1"
                     /translation="MAIIKSAYEIAMENSKNIQGDKELVEANKLRDEGKKMVSRLLDE
                     PSFDVKAALKAYDAKKLALVREGLMQSLLANLVLPSDEFTQNNTKRIGQAISAMVSDA
                     RKVSLIFSQLDNFFKEYLEERKRVVEAVERQYGPKLRKKEDELSRQMGRPVKINPAAD
                     PEYQALVRQYLSQLDAKYGEV"
     gene            complement(601..1200)
                     /locus_tag="VFH83_12290"
     CDS             complement(601..1200)
                     /locus_tag="VFH83_12290"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET6487196.1"
                     /translation="MTKGELRREIQSRLRRIAREDLSARSRRVAIRLQAEAAWKTADS
                     ILCFLSMPHEIETAAIIEAAVRDHGAAAVPRIEGERIRFVLLPSGEPDLERDRFGIPV
                     PGPDWPQWDSDGAGRILVVTPGLAFDRDGNRLGRGKGYYDRFLQECRARVGDRLVALG
                     VCFEEQLVEAVPHTERDQTVDGVVTEARTVAAARDSPRP"
     gene            complement(1197..>2125)
                     /locus_tag="VFH83_12295"
     CDS             complement(1197..>2125)
                     /locus_tag="VFH83_12295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HET6487197.1"
                     /translation="GRYALPAFNFVCLEQMLAIRDACVETASPFILQCSAHVRASIGP
                     AMVVRMAQGCIEGMRGLGRMVPMALHLDHGMTEADCKACIDDGFSSVMIDGSVLAFDE
                     NADLTARVVRYAHERDVTVEGELGTVGGAEDAALQAESRYTDPARVAEFVRRTGVDSL
                     AVSIGTSHGVVKIKAKEGEPLPSLRFDILEAIEKDLPGFPIVLHGASALPPRYVAMLN
                     QYGGRLTEAQGIPEAQTARAARSAVRKVNVASDGWIAMSAAVRKALAENPASIDPRVF
                     LSKARQEMKDVYVRKIVDVLGSAGKAPGGPAA"
BASE COUNT          328 a          745 c          660 g          392 t
ORIGIN      
        1 gcacctcgcc gtacttggcg tcgagctgag agaggtactg ccggaccaac gcctggtact
       61 ccgggtccgc ggcggggttg atcttcaccg gccggcccat ctgtcgggag agctcgtctt
      121 ccttcttgcg gagcttgggc ccgtactggc gctcgaccgc ctccacgacc cgtttgcgct
      181 cctccaggta ctccttgaag aagttgtcga gctggctgaa gatgagggag actttgcgag
      241 cgtcactcac catggcggag atggcctggc cgatgcgctt ggtgttgttc tgggtgaact
      301 cgtcggacgg gagcacgagg ttggccagca gggattgcat cagaccctcc cggaccaggg
      361 ccagcttctt ggcgtcatag gccttgagcg ccgccttgac gtcgaacgag ggttcgtcca
      421 ggagacggga caccatcttc ttgccttcat cgcggagctt gttcgcctcc acgagctcct
      481 tgtccccctg gatgttcttg gagttctcca tcgcgatttc gtacgccgat ttgatgattg
      541 ccatgtcgtg ccactctccc ttgccggttc atggcaatgt accgtgccga tcccgctgaa
      601 tcaaggccgc ggcgagtccc tggcggccgc gacggtccgc gcctccgtca ccacgccgtc
      661 caccgtctgg tccctctcgg tgtgcggtac cgcttcgacg agctgctcct cgaagcagac
      721 ccccagcgcg accaggcggt ccccgacgcg cgcccggcac tcctgcagga agcggtcata
      781 gtatcccttg cctcgaccca ggcggttgcc gtcccgatcg aacgccaggc ccggtgtcac
      841 caccaggatc cttccggctc cgtcgctatc ccattggggc cagtccggac cgggcacggg
      901 gatgccgaac cgatcgcgct ccaggtccgg ttccccggac gggagcagca cgaagcggat
      961 ccgctcaccc tcgatacgcg gcaccgccgc ggcgccgtga tcccggaccg ctgcctcgat
     1021 gatcgccgcc gtctcgatct cgtgcggcat ggagaggaag cagaggatcg agtccgcggt
     1081 cttccacgcc gcctccgcct gaaggcggat ggcgacccgc cggcttcggg ccgacaggtc
     1141 ctcccgcgcg atcctccgca gcctggactg aatctcgcgc cgaagctccc ctttggtcat
     1201 gccgcgggtc ccccgggagc cttgccggcg ctccccagca cgtccacgat cttgcggacg
     1261 tacacgtcct tcatctcctg tcgcgccttg gagagaaaca cgcggggatc gatggaagcc
     1321 gggttctccg cgagggcctt tcgaaccgcg gccgacatgg cgatccatcc gtcggaagcg
     1381 acgttgacct ttcggaccgc agaccgggca gcccgcgcgg tctgcgcctc cgggatcccc
     1441 tgggcctccg tgaggcgtcc cccgtactgg ttcagcatgg ccacgtagcg gggcgggagc
     1501 gccgaagctc cgtgcaggac gatgggaaaa cccgggaggt ccttctcgat ggcctccagg
     1561 atatcgaacc gtagcgatgg gagcggctcc ccctctttcg ccttgatctt cacgactccg
     1621 tgcgacgttc caatggagac cgccagactg tccacgcccg tccgccggac gaactcggcc
     1681 acccgggccg ggtccgtgta gcgggactct gcctgaagcg ccgcatcctc ggcgccccca
     1741 acggtgccca gctcgccctc cacggtcacg tcccgttcgt gcgcgtagcg gaccacccga
     1801 gccgtgaggt ccgcgttctc gtcgaacgca agcacggacc cgtcgatcat gacggaagaa
     1861 aaaccgtcgt cgatgcaggc cttgcagtcc gcctccgtca tcccgtggtc cagatggagc
     1921 gccatgggga ccatccgtcc gaggccgcgc atcccttcga tgcagccttg ggccattctt
     1981 acgaccatcg ccgggccgat ggaggctctg acatgagcgg agcattggag gatgaagggt
     2041 gatgccgtct cgacacaggc gtcccggatt gccagcatct gctccaggca cacgaagttg
     2101 aatgccggca gcgcatatct tcctt
//
LOCUS       DASRZS010000503         4227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3347529, whole genome shotgun sequence.
ACCESSION   DASRZS010000503 DASRZS010000000
VERSION     DASRZS010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3347529"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..441)
                     /locus_tag="VFH83_12300"
     CDS             complement(235..441)
                     /locus_tag="VFH83_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487198.1"
                     /translation="MENPMVRGPHYHSAAKEFARFCRINRVFMVDGLEVWRAALRARE
                     LPEDEIDQKIAAAAEFVRRMGEVS"
     gene            complement(534..1436)
                     /locus_tag="VFH83_12305"
     CDS             complement(534..1436)
                     /locus_tag="VFH83_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6487199.1"
                     /translation="MPDDPVFDCLFLGAYTRDTIWNGTSRRTVDGGAFYFGASVAARM
                     GLRVAAVTRMAPEDGDVLAELRSLGVAVEARPSHTSTRLDILYPSTNPDERVIRVRSS
                     AGPFSPNDLGSLRARIAIVGASFRGEVGEDVLATLVARQCAIALDAQGFVRALRDGVL
                     CQRKWPEAERLLPRVSILKADVNEAETLTGSRDFRAAASIIRSKGAAEALITSKEGVL
                     AADQTGYHEAVFLPRRVVGRSGRGDTCLAAYAARRLTHPAAEAVIWAAAVTSIKLETD
                     GPFRGGPAEVESRAEEIRSAIRSG"
     gene            complement(1429..2526)
                     /locus_tag="VFH83_12310"
     CDS             complement(1429..2526)
                     /locus_tag="VFH83_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487200.1"
                     /translation="MANAHVRIVEVSKSFGSTHALSRVSLEIPRGSFTTFLGPSGCGK
                     TTLLRIIAGLQEADGGSIFIGPRMVNGTASHLRGAVMVFQDYALFPHLTAAQNIAYGL
                     RVRKLPGAEIHSRVARIASYLGVEELLDRRPAQLSGGQQQRIALARALVTEPEVLLLD
                     EPLSNLDARLRETVRAELRRVQRSLGITTIYVTHDQTEALSMSDQLVVMDRGTVVQTG
                     SPRQVYSAPRNAFVASFVGTANLFPARVLRSARPVALCGTRGSTIPVKTQGAELAVGS
                     AVVLSVRPEHIRLSTEAPPASSEHQAVIQGVVTACLFEGSRARYWIEAMGREVVVDAP
                     GADEGDELRGEVFLTFDADRVHLMREEPRDA"
     gene            complement(2530..>4227)
                     /locus_tag="VFH83_12315"
     CDS             complement(2530..>4227)
                     /locus_tag="VFH83_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET6487201.1"
                     /translation="TVGPEGSGGQPGRLEEALGGHHRKVSRTSRVQGVSEPSLVVGVA
                     ILSACLLVFVLAPLAATLAFPNVGSWGVTLRSGRFAKAALHSLLMTILSTATATLVGF
                     LFAYTITRTATPLRGLFRAVSVLPLFSPPFMVAFGYVMMVGRNGLITHGLLGLRVNVF
                     GWHGLWLAQTISFFPVAAIVMEGALRSLSPNLLHAASNLGASGARQLWTITLPILRPA
                     FAAAGLLVGIQVLADFGNPILIGGNFSVLATEAWLRVEGWADVQGAAVLSAEILLPSL
                     LLFAAQRIWVRGRTVATVTARAGMDTVPRTSAAGRWLLGIVCGIVSALVLLVYVALLL
                     GAFVKGWGFDWTPTLANFGDITVRGAELLHSILYAAGAALGSAVVSLGTAFLVSRKRF
                     RLRSAMDLAALLPAALPGICFGIGYSILFSRPPLDLYGTAAIVVLSLLFWNISVGHQT
                     FVTAFGQISPAFGEAAANLGAGSFRVLREIELPLVSGAFLSAATVSFIRAVTTLSVVV
                     FLANAGNGVATVSIMNLVNDGFYGKAAALTVSLLVLSAGALGLGRLAAGRPVDLFQA"
BASE COUNT          781 a         1407 c         1419 g          620 t
ORIGIN      
        1 agaacgactg gacgttcggc atccgattcg actactcgta tagcgcaaag ttctagggcc
       61 ctgcggcgcc ccacggggtg ccgcttccga cgtgctcgcc ggtagcctct gctgccgtga
      121 acccctttct ctcttgccca ctttccgggc caccacggag agtgggctct tgtttttcag
      181 agacggttgc gcgagccagc ggcgggaggc cggcgagagg ctacaagcgc cacctcagga
      241 cacctctccc atccgtcgga caaactccgc cgcggccgct atcttctgat cgatctcgtc
      301 ctcaggaagc tcccgcgctc ggagcgccgc ccgccagacc tccagcccat cgaccatgaa
      361 gacacggttg atcctgcaga atctggcaaa ctctttggca gcgctgtgat aatggggacc
      421 ccgcaccatc gggttttcca tggcggacta ctcccagttc agagtgtgac gggtgacttt
      481 agtataccac cgcgcgccgc cagcgactat tccacggctg cagggatcga aggctaccct
      541 gatcggatgg cagagcggat ttcctctgcc ctggactcga cctccgctgg tcctccccgg
      601 aacgggccgt ccgtctccag cttgatgctg gttactgccg cggcccaaat caccgcctcg
      661 gcagcgggat gagtcagcct gcgcgccgcg taggcggcca gacaggtgtc accccggccg
      721 ctgcgaccca ccacccgacg gggcaggaac accgcctcat gataaccggt ctggtccgcg
      781 gccagaacgc cttccttgct cgtgatcagc gcctccgcgg cgcccttgga gcggatgatg
      841 gaggcagcgg cccgaaagtc ccggctgccg gtgagcgttt cagcctcgtt cacgtcagct
      901 ttgaggatgg acacccgggg cagcagtctc tcagcttctg gccacttcct ttggcaaagc
      961 actccgtccc gcagcgcccg cacgaaaccc tgggcgtcca gcgcgatcgc gcattgtcgg
     1021 gcgacgaggg tcgccaagac gtcttccccc acctcgccgc ggaaggaggc gccgacgatc
     1081 gctatccgcg cgcgtaggga gccgagatcg ttgggcgaga aagggccggc ggatgaccgg
     1141 acccggatca cgcgttcatc cgggttggtg gaggggtaga ggatatccag ccgagtggag
     1201 gtgtgggagg ggcgggcctc gacggcaacc ccgagggatc gcaactcggc cagaacgtcc
     1261 ccgtcttctg gggccatccg agtgaccgct gccacgcgca gccccatccg tgccgcaacc
     1321 gaggctccga agtagaaggc gcctccgtcc accgtgcgac gggaggtgcc gttccagatg
     1381 gtatctcggg tataggcgcc caggaacagg cagtcgaaaa ccggatcgtc aggcatcccg
     1441 cggctcctcg cgcatcaggt ggactcgatc cgcgtcgaag gtgaggaaga cctcgccgcg
     1501 gagctcgtcg ccttcgtcgg ccccgggggc gtccacgacc acctcccgac ccatcgcctc
     1561 gatccagtaa cgggcgcggg acccttcgaa gaggcaggcg gttacgacac cctggatcac
     1621 cgcctggtgc tcggaggatg ccggtggcgc ctccgtggag aggcgaatgt gctcgggcct
     1681 cacggagagg acgaccgcgg accccaccgc cagctcggcg ccctgggtct tcaccgggat
     1741 cgtagacccc cgggtgccgc agagggcaac cggccgggcg gatcggagga ctcgcgccgg
     1801 gaacaggttc gccgttccga cgaaggaagc gacaaaggca ttgcgagggg cggagtagac
     1861 ctgcctcggt gagcccgtct ggaccaccgt cccccggtcc atgaccacca gttgatccga
     1921 catcgacagc gcctcagtct ggtcgtgggt cacgtagatc gtcgtgatcc cgaggctccg
     1981 ctgaacgcgt cgaagctcgg cgcgcacggt ctctcgaagc cgggcgtcca ggttcgatag
     2041 aggctcgtcc aggagcagta cctccggctc cgtgacgagg gcgcgcgcaa gggcgatccg
     2101 ctgctgctgg ccgccggaga gctgcgcagg ccgtcgatcc agaagctctt cgacccccag
     2161 ataggaagcg atccgcgcca cccggctgtg gatctcggcg cccggcagct tgcggacccg
     2221 cagcccgtag gcgatgttct gcgcggcggt gaggtggggg aacagggcgt agtcctggaa
     2281 gaccatgaca gcgcctcgga ggtgggacgc cgtcccgttc accatccggg ggccgatgaa
     2341 gatgctcccg ccatccgcct cctggagacc ggctatgatc cgaaggagcg ttgtttttcc
     2401 acacccactg gggccgagga acgtcgtgaa gctgccccgt gggatctcca gcgagacccg
     2461 ggagagggcg tgcgtcgaac cgaagctctt gctcacctcg acgatgcgga cgtgcgcgtt
     2521 ggccatgcct tacgcctgga acaggtccac gggccttccg gcagccaggc gcccaaggcc
     2581 cagggcgccg gcggacagga ccaggagaga gacggtcagc gccgcggcct tgccatagaa
     2641 cccgtcgttc accaggttca tgatgctgac ggtggctacg ccgttgcccg cgttcgcaag
     2701 gaagaccacg acgctcaggg tagtcacggc ccggataaag gagaccgtgg cggcggatag
     2761 gaacgcgccg ctcaccagcg gcagctcgat ttcccgcagc acccggaagc ttccggcgcc
     2821 gaggttggcg gccgcctccc cgaaagccgg ggagatctgg ccgaacgctg tgacgaaggt
     2881 ctggtgtccg acggagatgt tccagaagag gagagagagc actacgatgg cggcggttcc
     2941 gtacaggtcc agcggcgggc ggctgaagag gatggaatac ccgatcccga agcagattcc
     3001 tgggagtgcg gctgggagca gcgccgcgag gtccatggcg cttctcaacc ggaagcgttt
     3061 gcggcttacc agaaaggcgg tgcccagaga gaccaccgcg ctgcccagag cggcgccggc
     3121 ggcgtacaag atgctgtgca gcagctcggc gccgcggacc gtgatgtcac caaagttggc
     3181 gagcgttggc gtccagtcga atccccaacc tttcacgaag gcgcccagca ggagtgccac
     3241 gtacaccaga agaaccagcg cggagacgat cccgcagacg atccccagga gccatcggcc
     3301 ggcggcggag gtcctgggaa ccgtgtccat gccggcccgc gccgtcacgg tggctaccgt
     3361 tcgcccgcgg acccagatcc gctgagccgc gaagagcagc agggaaggca gcaggatctc
     3421 cgccgaaagc acggcggctc cctggacgtc ggcccagcct tcgacccgca gccacgcttc
     3481 cgttgccaga accgagaagt tgcccccgat caggatgggg ttgccgaaat ccgcaagcac
     3541 ctggatcccg accaggagcc ccgcagccgc gaaagccgga cgaaggattg gaagggtgat
     3601 ggtccaaagc tggcgcgccc ctgaagcgcc gagattgctg gcggcgtgca gcaggttcgg
     3661 agagagggag cggagggccc cctccatgac gatggcggcg accgggaaga acgagatggt
     3721 ctgcgccagc cagagaccgt gccagccaaa cacgttcacc cgcaggccca ggagcccgtg
     3781 ggtgatgagc ccgttcctgc caaccatcat cacgtagccg aacgcaacca tgaagggcgg
     3841 ggagaacaac ggaagcaccg agacggcccg aaacagaccc cgcagcggcg tggcggtccg
     3901 ggtgatggtg taggcgaaga ggaaaccgac cagggtcgcc gttgccgtcg acaggatcgt
     3961 catgagcagc gagtgcagcg ccgccttggc gaagcgcccg gaccggagcg tgactcccca
     4021 ggacccgacg ttcggaaagg ccagcgtggc ggccagcggc gccagaacga acacgaggag
     4081 acacgccgac aggatcgcca cgccgacaac cagcgagggc tccgacacgc cctggactcg
     4141 agaggtgcgg ctcactttcc ggtgatgtcc gcccagcgct tcttccaggc gtccaggttg
     4201 accgccgctg ccttcaggtc ccacggt
//
LOCUS       DASRZS010000504         4995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4896661, whole genome shotgun sequence.
ACCESSION   DASRZS010000504 DASRZS010000000
VERSION     DASRZS010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4896661"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFH83_12320"
     CDS             <1..344
                     /locus_tag="VFH83_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487202.1"
                     /translation="SISFSSLANAHLTYQTLVTSASDVLTNADLQQLLAQAAGTPAAA
                     PTPGAQAAAAPAATTPAAAAPAAAPAAAPAAAAVAPPTPPAQQLTITDAKKKQLLLAY
                     LNRLIDIIFNQ"
     gene            423..1646
                     /gene="folP"
                     /locus_tag="VFH83_12325"
     CDS             423..1646
                     /gene="folP"
                     /locus_tag="VFH83_12325"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET6487203.1"
                     /translation="MASILLRSSATNAHALDIGSAEDLKRHLGEIDLSAEEIEKVTDQ
                     FLHCTIKLEGVDTRAANLLQSLLANLGGGLAMRKEAREFTVRETDVLLTGSHHTLSLL
                     AHRLKGEPFGLEQVGAEILACMASENRVMSWGSRTLDFARRTYVMGILNCTPDSFFPG
                     SRSATLKDAMKNAEEMIEAGVDIVDVGGESTRPGAEPVAEEEEIRRVIPCIQSLRAVS
                     DVMISVDTRKREVAERALDAGADIVNDISGLRHNDDLARLVARRKVPIVLMHLRGTPR
                     TMQKQAYYKNTISEILRELQPSIAAAIAAGIAPDMIIIDPGIGFAKHIQDNLRIIKEL
                     ASLKSLSFPILIGLSRKKFIGEILDRPVERRLIGTITANTLAVINGANIIRVHDVGDA
                     VEMVKIIDSVRRIGF"
     gene            1655..2440
                     /gene="cdaA"
                     /locus_tag="VFH83_12330"
     CDS             1655..2440
                     /gene="cdaA"
                     /locus_tag="VFH83_12330"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HET6487204.1"
                     /translation="MDQLLNTWAVREVIRPALDVLMLSYLLYKSWQILVQTRAVQLLK
                     GTVVMLLIYGVSFFLRLTTLLWILNVLVPGLVIAIAIIFQPELRKIFTQIGSRQWFRT
                     AVQGGTRQLETVISAVELLSTRRRGALIVFPRRVGLKNIIQTGTVLNAAISTNLITTV
                     FFEGTPLHDGAMVIDGDRVVAAGCFLPLSDREDMGSTFGARHRAALGLVEETDALVVV
                     VSEENGAVSLAHDGNLRYNLTLAEVETGLSGLLRPERHPEADR"
     gene            2437..3384
                     /locus_tag="VFH83_12335"
     CDS             2437..3384
                     /locus_tag="VFH83_12335"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HET6487205.1"
                     /translation="MRSRRLARTLLADLPVKVICLAAAVVLFLFHRVNTLTERFFSVP
                     LEVTTPSGLAVASAFPKTVRITLRGAEEAIFPILEEDIEASVDLDTYRSAGIYQAQVK
                     VNRKGTALNVEPLEINVDPGQITFTLEPLTERRVSVIPDIRGEPAYGYELVQSTVSPS
                     SVAIRGARSRVQGVSAVPTEEIDLTGRTGSFASRVRILLPNALLRIAGDPSVDFKATI
                     QESIVTRRFDAVPISTVDLSPHLTLRSPIPPGSVQVQGAQLVVDGVRPEQVRLVLDCS
                     AVKRAGQYELHPRAEVPSSVTVPDFDPKVVTVEFVVSGG"
     gene            3395..3775
                     /locus_tag="VFH83_12340"
     CDS             3395..3775
                     /locus_tag="VFH83_12340"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET6487206.1"
                     /translation="MILGVGIDVVHVERIRRWRSIEGLYERFFHPEELASLLPRGEAG
                     IISLAARFAAKEAFGKAIGSGLRHFALRDIAVLNDSNGKPHLMLVGAAETAFKELGGK
                     RLVCSLSHERDNALAVVIIEGEAP"
     gene            3772..4686
                     /locus_tag="VFH83_12345"
     CDS             3772..4686
                     /locus_tag="VFH83_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HET6487207.1"
                     /translation="MNDTQKLTFNERKQTNCPVCQGKFYREDLLSGGGRLIAGELTAE
                     LRRVYEPSKKYGEVHPLVYSISVCPVCYYSALPADFSNLSESAIHKADTASDARRRSI
                     SLLFPSLDYHAPRTLREGLASYFFAMQCYELFDRKANPTFKAGLCALRGAWLAGDLQK
                     QEPDQNWGALATLFYRKARFYYRESLERESGGQEPFEPNLAFGPDLDKNYGYHGVIYL
                     SAYLDYRHGPRSDAAARVASIEYARRMMAKVFGMGKTSKNRPSALLDKAKEIYEAIGV
                     EVEKLQGTAAVESVAAAADPTAGIDGAL"
     gene            4696..>4995
                     /locus_tag="VFH83_12350"
     CDS             4696..>4995
                     /locus_tag="VFH83_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET6487208.1"
                     /translation="MPERNIRLTLAYDGTDFLGWQQQASGRTVQGVLLEGLGRMHGHP
                     VRIVAAGRTDAGVHATGQVANFHSDIDSIPPDRYRDAINSYLPRDVRVLESQEADP"
BASE COUNT          845 a         1658 c         1644 g          848 t
ORIGIN      
        1 gatcgatcag cttctccagt ctcgcgaacg cgcacctcac ctaccagacg ctcgtgacat
       61 cggcttccga tgtgttgacc aacgcggacc tccagcagct cttggcgcag gcggcgggca
      121 cgccggccgc cgcgcctacg ccgggggctc aggctgccgc tgccccggcc gccaccacgc
      181 cggccgctgc cgccccggct gccgcgccag ccgccgcccc ggccgccgcg gccgttgcgc
      241 cgccgacgcc tcccgcacag cagctcacca tcacggacgc caagaaaaag cagctcttgc
      301 ttgcctacct gaaccggctc atcgacatca tcttcaacca gtgaccgtcc cgccatcccg
      361 ttccctcccg ccggagcggt ttggcttgac gctttcggaa ggcggctgtt agtatccgga
      421 ccgtggcctc catcctcctt cggtcttcag cgaccaacgc gcacgcactg gatatcggct
      481 cagcggaaga cttgaagcgg cacctcgggg agatcgatct cagcgccgag gagatcgaga
      541 aagtcaccga ccagttcctg cactgcacca tcaagctgga gggcgtggac acgcgcgcgg
      601 ccaaccttct gcagagcctc ctggccaacc tgggcggtgg gcttgccatg cgcaaggagg
      661 ctcgggagtt caccgtccgg gagacggacg tgctcctcac gggcagccat catacccttt
      721 cgctgcttgc gcatcgtctg aagggtgagc cgttcggcct ggagcaggtc ggcgcggaga
      781 tcctggcctg catggcgtcg gagaaccggg tgatgagctg gggatcccgc accctggact
      841 tcgcccgcag gacctacgtc atgggtattc tgaactgcac cccggattcg ttcttcccgg
      901 ggagccgctc cgcgacgctc aaggacgcca tgaagaacgc tgaggagatg atcgaggcgg
      961 gcgtggacat tgtcgacgtg ggcggggagt ccacacgccc gggcgccgag cctgttgccg
     1021 aggaagagga gatccgacgg gtcatcccct gcatccagtc gctgcgcgcc gtttcggacg
     1081 tgatgatctc cgtcgatacc cgcaagcgcg aggtggcgga acgggcgctg gacgccgggg
     1141 cggacatcgt caacgacatc tcgggtcttc ggcacaacga cgatcttgcc cggctggtgg
     1201 cgcgccgcaa ggtgcccatc gtgctgatgc atctccgcgg cacgccgcgc accatgcaga
     1261 aacaggccta ctacaagaac acgatcagcg agatcctccg agagctgcag ccctccatcg
     1321 cggcggccat cgccgcggga atcgctccgg acatgatcat catcgacccg gggatcggct
     1381 tcgccaagca tatccaggac aacctgcgga tcatcaagga gctggcttcc ctcaagagcc
     1441 tcagcttccc gatcctgatc gggttgtccc gcaagaagtt catcggtgag atcctggatc
     1501 ggccggtgga gaggcgcctg atcgggacca tcacggccaa taccctcgcg gtcatcaacg
     1561 gcgccaacat catccgcgtc cacgacgtgg gagacgcggt ggagatggtg aagatcatcg
     1621 actcggtccg caggatcggg ttctagggga gcccgtggat cagcttctga atacctgggc
     1681 cgttcgggag gtcattcggc cggctctgga cgtgctcatg ctctcgtacc tgctgtacaa
     1741 gagctggcag atcctcgtgc agacgcgcgc cgtgcagctc ctcaagggca ccgtggtgat
     1801 gctcctcatt tacggggtgt ccttcttcct gcgcctgacc acgctgctgt ggatcctgaa
     1861 cgtcctggtc ccggggctgg tcatcgccat cgccatcatc ttccagccgg agctgcgcaa
     1921 gatcttcacc cagatcggca gccgccagtg gtttcggacc gcggtccagg gaggcacgcg
     1981 ccagctggag accgtgatca gcgcggtgga gctgctctcc acgcggcgcc gcggcgccct
     2041 gatcgtgttc ccccgacggg tggggctgaa gaacatcatc cagaccggca cggtgctgaa
     2101 cgccgcgatc tccaccaacc tcatcacgac cgtcttcttc gagggaaccc ctctgcacga
     2161 cggggccatg gtgatcgacg gcgatcgcgt tgtggcggcg ggctgcttcc tcccgctctc
     2221 ggatcgggag gacatgggga gcaccttcgg ggcccggcat cgcgcggcgc tgggtctcgt
     2281 agaggagacc gacgcgctgg tggtggtggt ttcggaggag aacggcgcgg tatcgctggc
     2341 tcatgatggc aacctccggt acaacctcac gctggcggag gtggagacgg gtctctccgg
     2401 cctccttcgg cccgagcgcc atcccgaggc ggaccgatga ggagccgccg gctcgcgcga
     2461 acgctcctgg ctgacctgcc ggtgaaggtg atctgtctgg cagccgccgt ggttctcttc
     2521 ctctttcacc gggtcaacac gctgacggag cggttcttca gcgtgcctct ggaggtgacg
     2581 acgccctcgg gcctcgccgt agcgtcggcc ttccccaaga cggtccgcat caccctccga
     2641 ggcgcggagg aggccatctt cccgatcctg gaggaggaca tcgaggcttc cgtggatctc
     2701 gacacctatc gaagcgcggg catctaccag gcgcaggtca aggtgaaccg caagggcacg
     2761 gccctcaacg ttgagcccct ggagatcaac gtagatcccg gccagatcac cttcaccctg
     2821 gagcccttga cggagcgccg ggtcagcgtg attccggaca tccgcggtga gccggcctac
     2881 gggtacgagc tggtgcagtc cacggtgagc ccctcgagcg tcgccatccg cggggcccgc
     2941 agccgggtgc agggggtgag cgcggtgccc accgaggaga tcgatctcac cgggcggacc
     3001 gggagctttg cctcacgcgt gaggatcctc ttgcccaacg cgctcctgcg gatcgccgga
     3061 gatccgtcgg tcgacttcaa ggcaacgatc caggagtcca tcgtgacccg acgcttcgat
     3121 gcggtgccga tatccacggt ggacctctcg ccccacctga ccttgcggag tcccatcccg
     3181 ccgggcagcg tccaggtgca gggcgcgcag ctcgtggtgg atggggttcg gcccgaacag
     3241 gtgcggctcg ttctggactg ctcagcggtc aagagggccg ggcagtacga gctgcatccg
     3301 cgcgccgagg tcccctcctc cgtcaccgtc ccggactttg accccaaggt cgtcacggtg
     3361 gagttcgtcg tatccggggg atgaggaggc tggcgtgatc ctgggtgttg ggattgacgt
     3421 cgtgcacgtg gagcgcatcc ggcggtggcg ctccatcgag ggtctgtacg agcggttttt
     3481 ccatccggag gagctggcgt cgctcctgcc ccggggagag gccggcatca tctcgctcgc
     3541 ggcgcgcttc gccgccaagg aggccttcgg gaaggccatc gggtcggggc tgcgccactt
     3601 cgcgctgcgc gatatcgcgg tcctgaacga ctccaacgga aagcctcacc tgatgctggt
     3661 gggggcggcg gagaccgcgt tcaaggagct tggcggcaag cgcctggtct gctcgctctc
     3721 ccacgagcgg gacaacgcgc tggccgtggt catcatcgaa ggagaggcgc cgtgaacgac
     3781 acgcagaaac tcaccttcaa cgagagaaag cagaccaact gtcccgtgtg ccagggcaag
     3841 ttctaccggg aagacctgct ctccggaggg gggcgcctga tcgcgggaga gctcacggcg
     3901 gagctccggc gcgtgtacga gccgtccaag aagtacggcg aagtccatcc cctcgtctat
     3961 tccatcagcg tctgtccggt ctgctactat tccgccctgc ccgcggactt ctccaacctc
     4021 tccgagtcgg ccatccacaa ggcggacacg gccagtgatg cccgccgccg aagcatctcg
     4081 ctgctctttc cgtcactgga ctaccacgcc ccgcgtactc tgcgggaggg gcttgccagc
     4141 tacttcttcg ccatgcagtg ctacgagctc ttcgaccgca aggcgaaccc caccttcaag
     4201 gcggggctgt gcgcgcttcg cggcgcgtgg ctggccgggg acctccagaa acaggagccg
     4261 gaccagaact ggggagcgct cgccacgctc ttctatcgca aggcgcgctt ctactatcgg
     4321 gagtcgctgg aacgggagag cggggggcag gagcctttcg agcccaacct ggcttttggc
     4381 ccggacctcg acaagaacta tggctaccat ggggtgatct atctgtctgc ctacctcgac
     4441 taccgtcacg gcccccggag cgacgccgcg gcccgggtgg ccagcatcga gtatgcccgc
     4501 cgcatgatgg cgaaggtatt cgggatgggg aagacttcga agaatcggcc gtccgcgctc
     4561 ctggacaaag ccaaggagat ctacgaggcc atcggcgtgg aagtggagaa gctgcagggc
     4621 accgcagcgg ttgagagcgt cgcggccgcg gccgatccaa cggccggtat cgacggggcg
     4681 ctctgatcga ctgagatgcc cgagcgaaac atccggctca cgctcgcata cgacggcacc
     4741 gacttcctgg gctggcagca gcaggcaagc gggaggaccg tccagggcgt tctgctcgag
     4801 gggctcggtc gaatgcacgg acatccggtt cggatcgtgg cggcggggcg gacggacgcc
     4861 ggcgtccacg caaccgggca ggtggccaac ttccattcgg acattgactc catcccgccg
     4921 gatcgctatc gggatgccat caactcgtac ctgccgcggg acgtgagggt gctggagagc
     4981 caggaggcgg atcca
//
LOCUS       DASRZS010000505         3202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3741903, whole genome shotgun sequence.
ACCESSION   DASRZS010000505 DASRZS010000000
VERSION     DASRZS010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3741903"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VFH83_12355"
     CDS             <1..1155
                     /locus_tag="VFH83_12355"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487209.1"
                     /translation="RLPQDIDTDTLLDYLDKKWDAQGTYLQAYGLYVLSAYGRQETAK
                     AILTRLKSFLRPGTRTVTLVGTVNDRLWYGGDVQAKSLLLMLYSKLQPDSQLVLGLAN
                     DLLSGNRAGSWGNTSNAGWVLQAFAEMIAAGGEENADFTASVKLGNAVIASNPFKGMS
                     TTPFTAKLDAPKLADTAASQQGPAVPGGKQLPLTFAVDGTGTLYYTAEMRYALATDQV
                     DARDEGLGLGMDLLDETGAPVEGTNLVLGSVYQMRIVFYSSKDRTYLAIRAPIPSGAE
                     PIDGSLVTSQTPKPQAEDQSDQTDQAGDQGNVPPDYGYTGPSTRIYDSEVRFFFDELP
                     RGRHQVTFTFRCTTPGVFPTPPVQAELMYQPEVFGRTAGATYTIAPASDQ"
     gene            1163..>3202
                     /locus_tag="VFH83_12360"
     CDS             1163..>3202
                     /locus_tag="VFH83_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HET6487210.1"
                     /translation="MNGKRRVHIVPHTHYDAEVFLSAEETFEIGFRNIELALRLLEAD
                     RSYTFSLDQVCYVRPYLERYPEKREPVLRFLNEGRLTMEGDMHVMPDMNLPGGESFLR
                     QVLYGKQFFQNDLDTSSRCAWLLDSFGFHPQTPQLLAGCGYAWNLIQRGTPAEWPSDF
                     LWEGIDGTRMPVHNMPMGYAVFSGAPSNFHEFQGFAERRLAFLSEESSRSCLLALTGA
                     DLAAPDAHLPDMVARFNREQDAYELVFSTPERYRAELVETGELPVLKGDLNGVFHGCY
                     SARITVKQRNRELETALLDWEKVDALAAATAGKAEGAGEAAACEPDAARALQDAWEPV
                     LFNQFHDIICGSHVDVVYRATLDRFDFSEALARLGTDRALSALTRRIDTSGPGIAVVV
                     FNTLGHPRSDAAEVSFAFAQSDGFEVEVRDSAGRIVSSDLLSADRAADGSILRGRVLF
                     IAQDVPSFGYEVYHIVPLGPGSRPGGEAPSEIRTSAPLNLRRELDAGFLENSLLHLDF
                     DLWKGVITGLVHRGNDWQVISPEYPRGNTIVREQDFGNFWQYNGPCKGDLFHPPLDRG
                     VSRGLYPLPAENTNQASFSHRTDGDANIRTGSAFAELSVGHPWGTGHFTTRVRIFAGL
                     PRVDFLTMVLNNDERVRYRAVFPTSIRGGEIAHEIPFGAIDRPEGEYPAQTWMDYG"
BASE COUNT          542 a         1071 c         1045 g          544 t
ORIGIN      
        1 cgcctgccgc aggacatcga cacggacact ctgctggatt acctggacaa gaaatgggac
       61 gcgcagggaa cctacctgca ggcctacggg ctgtacgtgc tttccgccta cggccggcag
      121 gagacggcaa aggcgatcct cacgcgcctg aagagcttcc tgcgcccggg aacgcgcacg
      181 gtgaccctgg tgggaaccgt gaacgaccgg ctctggtacg gcggtgacgt gcaggcgaaa
      241 tccctcctgc tgatgctcta ctccaagctc cagccggact cccagctcgt cctgggtctt
      301 gccaacgacc tgctgtccgg aaaccgggcc gggtcgtggg gcaacacgag caacgccggg
      361 tgggtgctcc aggcgttcgc ggagatgatc gccgccggcg gtgaggagaa cgcggacttc
      421 acggcgtccg tcaagctggg gaacgcggtg atcgcctcca atcctttcaa gggcatgtcg
      481 accactccct tcaccgcgaa gctggatgct ccgaagctcg cggacacggc cgcatcccag
      541 cagggaccgg cggtgccggg gggcaagcag cttccgctca cctttgccgt ggacggaacc
      601 gggaccctct actacacggc ggagatgcgc tacgcgctgg ccacggacca ggtggatgct
      661 cgggacgagg ggctggggct tgggatggac ctgctggatg agaccggcgc cccggtggaa
      721 gggaccaacc tggtcctagg cagcgtctac cagatgagga tcgtattcta ctcctcgaag
      781 gatcggacct acctggccat ccgggctccc atcccctcgg gagccgagcc catcgacggc
      841 tccctggtta cgtcccagac cccgaagcct caggcggagg accagtccga tcagacggat
      901 caggccggag atcaggggaa cgtgccgccg gattacggct acaccggtcc cagcacgcgg
      961 atctacgaca gcgaggtgcg gttcttcttc gacgagcttc cccgcggcag acaccaggtg
     1021 acgttcacct tccgctgcac cacgcccggc gtgttcccga cgccgccggt gcaggcggag
     1081 ctgatgtacc agccggaggt gttcggtcga acggcggggg ccacctacac gatcgctccc
     1141 gcttcggacc agtgaggcgg agatgaacgg gaagcgccgg gtccacatcg ttcctcacac
     1201 ccactatgac gccgaggtct tcctctcggc ggaggagacc ttcgagatcg gcttccgcaa
     1261 catcgagctg gcgctgcgcc tgctggaggc ggaccggtcc tacacgttct ccctggacca
     1321 ggtctgctac gtgcggccct acctggagcg gtaccccgag aagcgggaac cggtcctgcg
     1381 ctttctcaac gagggtcggc tcacgatgga gggagacatg cacgtgatgc cggacatgaa
     1441 cctccccggc ggggagtcct tcctccggca ggtcctctac ggcaagcagt tcttccaaaa
     1501 cgatcttgac accagcagcc gctgcgcgtg gctcctggat tccttcggct ttcatcctca
     1561 gacgccgcag ctcctggccg gttgcggcta cgcgtggaac ctcatccagc gcggaacgcc
     1621 ggccgagtgg ccgtcggatt ttctgtggga agggatcgac gggacgcgga tgcccgtgca
     1681 caacatgccc atgggatatg ccgtcttctc cggagcgccg tcgaacttcc acgagtttca
     1741 aggcttcgcc gagcgcaggc tggcgttcct ctccgaggaa tccagccgaa gctgtctcct
     1801 ggccttgacg ggtgcggacc tcgccgctcc tgatgctcac ctgcctgaca tggttgctcg
     1861 cttcaaccgc gagcaggatg cgtacgagct cgtcttctcc actccggagc gctatcgggc
     1921 ggagctggtg gaaacgggag agcttccggt tctcaagggc gatctcaacg gcgtgttcca
     1981 cgggtgctac agcgcgcgga tcaccgtcaa gcagaggaac cgcgagctgg agaccgcgct
     2041 cctggactgg gagaaggtgg acgctctggc cgcggcgacg gctggcaagg cggagggggc
     2101 cggtgaagca gcggcctgtg agcccgatgc ggctcgggct ctccaggacg cctgggagcc
     2161 ggtgctcttc aaccagttcc acgacatcat ctgcggaagc cacgtggacg tcgtctatcg
     2221 ggccacgctg gaccggttcg acttctccga ggcactggcc cggctcggca cggaccgggc
     2281 cctttcggcg ctgacccggc ggatcgacac ctccgggccg gggattgcgg tggtggtctt
     2341 caacacgctg ggccacccgc gttcggatgc ggcagaggta tccttcgcct tcgcgcagtc
     2401 ggacggattc gaggtggagg taagggactc cgccggacgg atcgtttcct ccgacctcct
     2461 ctccgccgac cgggccgcgg acggttccat tctccgcggc cgcgtgctct tcatcgctca
     2521 ggacgttccc tccttcggct acgaggtcta ccacatcgtg cctcttggtc ccggatccag
     2581 accgggcgga gaggcgccgt cggagatccg gacctcggct ccgctcaacc tccgtaggga
     2641 gctggacgcg gggttcctgg agaacagcct gctccacctg gacttcgatc tctggaaggg
     2701 cgtgatcacg ggactggtgc atcgcgggaa cgactggcag gtgatctcac cagagtaccc
     2761 gcgcggcaac acgatcgtcc gggagcagga cttcggaaac ttctggcagt acaacgggcc
     2821 gtgcaagggc gaccttttcc acccgcccct ggaccgcgga gtctcccgag ggctctatcc
     2881 gctgcccgcg gagaacacca accaggcctc gttctcccac cggacggacg gagacgccaa
     2941 catccggacc ggctcggcgt tcgcggagct ctccgtcggc cacccttggg gaaccggcca
     3001 cttcacgacc cgggtccgca tcttcgccgg acttccccgg gtggacttcc tgaccatggt
     3061 cctgaacaac gacgagcgcg tccggtaccg cgccgtcttt cccacctcca tccgtggcgg
     3121 tgagatcgcg catgagatcc ccttcggagc catcgaccgg cccgagggag agtatcccgc
     3181 gcagacgtgg atggattacg gg
//
LOCUS       DASRZS010000506         5966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5284013, whole genome shotgun sequence.
ACCESSION   DASRZS010000506 DASRZS010000000
VERSION     DASRZS010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5284013"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFH83_12365"
     CDS             <1..482
                     /locus_tag="VFH83_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6487211.1"
                     /translation="DGRDPKAPAGCEGGFFLGPTIFDHVTEGMTVGWEEIFGPVLCLK
                     RVKDFEEGLAVMNANEFGNGSVIYTRSGYFAREFSKRSQAGMVGINVGIPVPLGVFGF
                     TGHKNSFFGDLHTMGADGVRFYTELKSVTAHWFSEEEAREGKVANSWDGMISFPDKK"
     gene            491..1765
                     /locus_tag="VFH83_12370"
     CDS             491..1765
                     /locus_tag="VFH83_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET6487212.1"
                     /translation="MDTVEKYKRYVNTAFVKAVEPVVAVSGKGSKLKAEDGKEYTDLF
                     GGISVVNAGHVNPEVAAAAKAQIDKLVHCCSYLYHVPPVADLAEKLAQITPGRLQKSF
                     FGNGGAEANEGAMRLAKQFTKKQEFISLQGSFHGRSLATLSITGNMARKRGGGPYLSG
                     CSYHPAPYCYRCPFQMSYPSCDLHCAKELEHTIQYSTSDNVAAFIAEPVMGEGGIITP
                     PPEYFTEVKKVLDAHGILFFADEVQSGFGRTGKLFAIEHYGVEPDIMTMAKGIADGFP
                     LSAFIARPEVADSFRPGDHLSTFGGNPVSCAAALANIAFFEREKLPEQAAAKGARVME
                     ELRGLARRSPLIGDVRGKGLMIGIELVRDKDKTPAAAEANRLRTLMREKGFLIGVGGT
                     WGNVLRWQPPLVISSQELTEAVGALGDSIKTL"
     gene            1776..2708
                     /locus_tag="VFH83_12375"
     CDS             1776..2708
                     /locus_tag="VFH83_12375"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HET6487213.1"
                     /translation="MKVGIIGAGAMGSLFGGKLSLRGHDVVLYDVYLEHVRHVASQGL
                     SIEDAQSGQVVVARPGASADPASVQGADILVVFVKSPSTEAVAAQFAPLAGPQSIALT
                     LQNGLGNEAILRDHFGPTRTAAGVTSQGATFLGPGKIRHAGVGPTHIAMADGRSERLR
                     EFADALTDAGFETHVEKDVAGLVWSKLVINVGINALTALMGQPNGRLLDFEESRQLMA
                     TLVEEAVSVAVRRGIRLSYPDPLATVMEVARKTGANRSSMLQDFDRGRQTEIDYMNGA
                     VVREAAAVGMKVPANETVTRLIKALERARVRPEA"
     gene            2705..3973
                     /locus_tag="VFH83_12380"
     CDS             2705..3973
                     /locus_tag="VFH83_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET6487214.1"
                     /translation="MIDATERLLLGTLDREELVSLIRDLVRIDSVIRPESGGTERGVV
                     RYLTDWIRKKLSVEPQIEEVSPGRENVVVTVDSGRSGPCLLLEGHTDVVSEGEREAWT
                     HDPFAADLAEGRIYGRGACDMKAGLAVNLLTAAAFCRAGASWAGRIRLALVCDEEGMM
                     SGIKHFIRSGHADGVDACLVPEPEENQLCIRMKGAIRAVVRVHGKMAHGAMPLTGINP
                     NPRLARIILAFERFEAEEKERCGADPYLGLPSVTFTVLQAPPSGVPAQLNVMPGEAVG
                     FVDIRTTLSQDHETVRSGLRAVLRSLASLDPDFNAQIEFIEDRPVVGIERDAPIAAIA
                     AAAYQDVTGREPTWNGVPGATDGTFLSAWKRIPCLVNGPGSRLIPHQADEYVDADQAL
                     ECARIYVLTASRFLAASGHPAASGSAVASV"
     gene            3970..4737
                     /locus_tag="VFH83_12385"
     CDS             3970..4737
                     /locus_tag="VFH83_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487215.1"
                     /translation="MSGAPVLEVKGIRKAFGAIKALNGVDITVAAGAIHGIIGPNGSG
                     KTTLFNCITGLLRADAGSVRLDGRDITSLPSHAIANGGIRRTFQAGKLVPGMTVLENV
                     MSGINDRIRVSAVDLLRAPFRASRREEGIRERARRALDLVGMPEAAERWAADLVWTER
                     QLVQIARALIAEPRVLLLDEPASGMGVKEAEKVERILRGARGRGMTVVVVSHDVKLLM
                     NLSDLVTVLNFGEKIAEGEPSVVQNDPRVLEAYLGTQ"
     gene            4748..5446
                     /locus_tag="VFH83_12390"
     CDS             4748..5446
                     /locus_tag="VFH83_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487216.1"
                     /translation="MLAVEDLSVSYGHVNALRGVTIDVRPGEIVVLIGANGAGKTTLL
                     ETILGINTPLRGRIRFDGESIGGVSADRNVRSGLCLVPEGRGVFASMTVLDNLLLGAH
                     HNLRNVSRNLDRVYEWFPVLEARREQIARTLSGGERQMLAIGRALMSAPRLIMVDEPS
                     IGLAPIVVNDIFSILTRLNKEGYTILLAEQNAYKALTCAHRGYVLETGVVSLSGDARA
                     LLEDPGVKAAYLGA"
     gene            5449..>5966
                     /locus_tag="VFH83_12395"
     CDS             5449..>5966
                     /locus_tag="VFH83_12395"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6487217.1"
                     /translation="MQVFVAQLINGLSLGSIYVLLVTGFNLLLLVAMIIHFTYPQVVV
                     FSMYITWVVLRATGNNLPLGILAGVASSIAMNLASAPFFQKVMQKRGAVDINSTMVLS
                     MGIGMIITDVLSHSFNQGLPIAFPTSLSSDTPLARFGVISIATGQVWALGVGIVAVTA
                     VFILLYRSRLGRA"
BASE COUNT         1023 a         1908 c         2031 g         1004 t
ORIGIN      
        1 tggacggccg cgatcccaag gcccccgccg gctgcgaggg cggattcttc ctcggcccca
       61 cgatcttcga ccacgtcacc gaagggatga ccgtggggtg ggaggagatc ttcgggcccg
      121 tgctctgcct caagcgggtc aaggatttcg aggaaggact cgccgtgatg aacgcgaacg
      181 agttcggaaa cggcagcgtg atctacaccc gcagcggata cttcgcgcgc gagttctcga
      241 aacgctctca agctgggatg gtagggatca acgttgggat cccggtgccc ctgggcgtct
      301 tcgggttcac cggccacaag aactccttct tcggcgacct gcacacgatg ggcgccgacg
      361 gcgtgcgctt ctacacggag ctcaagagcg tgacggctca ctggttctcc gaggaagagg
      421 cgagggaagg caaggtagcc aatagctggg acgggatgat ctcgttcccc gacaagaagt
      481 aggagaccgg atggacaccg ttgagaagta caagcgctac gtgaacacgg ccttcgtgaa
      541 ggccgtggaa ccggtggtcg cggtttcggg caagggcagc aagctgaagg ctgaggacgg
      601 caaggagtac accgaccttt ttggggggat ctccgtcgtg aacgcgggcc acgtcaaccc
      661 ggaagtggcc gccgcggcca aggcccagat cgacaagctc gtgcactgtt gctcgtacct
      721 gtaccacgtc ccgccggtcg cggaccttgc ggagaagctg gcgcagatca cgcccgggcg
      781 gctccagaag agctttttcg gcaacggcgg agcggaggcg aacgagggcg ccatgcgcct
      841 ggccaagcag ttcacgaaaa agcaggagtt catctctctc caggggagct tccacggccg
      901 gagcctggct acgctctcca tcaccgggaa catggcccga aagcgcggcg gcggtccgta
      961 cctgagcggc tgctcctacc atccggcgcc gtactgctac cgctgcccct tccagatgag
     1021 ctatccctcc tgcgaccttc actgtgccaa ggagctggag cacacgatcc agtacagcac
     1081 cagcgacaac gtggcggcct ttatcgccga gccggtgatg ggggagggag ggatcatcac
     1141 cccgcccccg gagtacttta cggaggtcaa gaaggtcctg gacgcgcacg gcatcctgtt
     1201 cttcgcggat gaggtgcagt caggcttcgg tcggaccggc aagctcttcg ccatcgagca
     1261 ttacggggtg gagccggata tcatgaccat ggccaaggga atcgcggacg gcttcccgct
     1321 ttccgcattc attgcccggc ccgaggtcgc ggactccttc cgccccggcg atcacctgtc
     1381 caccttcgga gggaaccccg tgtcctgcgc cgctgccctc gccaatatcg ccttcttcga
     1441 gcgggagaag ctgccggagc aggcggctgc caagggcgcg cgtgtcatgg aggagcttcg
     1501 agggctggcc cggcggagcc ctctgatcgg ggacgtgcgc ggcaagggcc tgatgatcgg
     1561 gatcgagctg gtgcgtgaca aggacaagac tccggccgcc gcggaggcca acaggcttcg
     1621 gacgctgatg cgggagaagg gattcctcat cggcgtcggc gggacctggg gcaacgtctt
     1681 gcggtggcag ccgcccctgg tcatcagctc ccaggagctg accgaggccg tcggcgcctt
     1741 gggcgacagc atcaagaccc tgtaggatcc ggttcatgaa ggtcggcatc atcggggcgg
     1801 gggccatggg ctccctcttt gggggaaagc tctctctgcg tggtcacgac gtggtccttt
     1861 acgacgtgta cctggagcac gtgaggcacg tggcctccca gggcctgtcg atcgaagatg
     1921 cccagagcgg acaggtggtg gtggcccgac cgggcgccag cgccgacccg gcgtccgtcc
     1981 aaggagcgga tatcctcgtc gttttcgtga agtccccctc cacggaagcc gttgccgcac
     2041 agtttgcccc gctggccggg ccccagagca tcgccctcac gctccagaac gggctcggaa
     2101 acgaagccat cctgagagat cactttgggc cgacccggac cgcggcgggc gtcacgtccc
     2161 aaggggccac cttcctgggc cctggcaaga tccgccacgc cggcgtcggc ccgacgcaca
     2221 ttgccatggc cgacgggcgg tccgagcgcc tgcgggagtt cgccgacgcc ctcacggacg
     2281 cgggttttga gacccatgtc gagaaggacg tggcggggct ggtctggagc aagctcgtga
     2341 tcaacgtggg catcaacgcc ctcaccgcct tgatgggcca gcccaacggg cgtctgctgg
     2401 acttcgagga gtctcgccag ctcatggcca ccctggtgga ggaggcggtt tccgtggcag
     2461 tccggcgcgg aatccgcctg tcgtacccgg atccgttggc aaccgtgatg gaggtggccc
     2521 gcaagaccgg tgccaaccgc tcctccatgc ttcaggactt cgaccgcggc cgccagaccg
     2581 agatcgacta catgaacggc gccgtggtcc gggaggccgc ggccgtgggg atgaaggtcc
     2641 ccgcaaacga gacggtgacg cgcctgatca aggcgctgga gcgcgcgcgg gtgaggcctg
     2701 aggcgtgatc gacgcaacgg agcgcctcct cctcgggacg ctggaccgtg aggagctcgt
     2761 gtccctgatc cgcgatctgg tccgcatcga ttccgtgatc cgaccggagt ccggcggcac
     2821 cgagcggggc gtggtgcggt acctgacgga ctggatccgg aagaagcttt ccgtcgagcc
     2881 gcagatcgaa gaggtgtccc cgggccggga gaacgtggtg gtcacggtgg actccggacg
     2941 ctcagggccc tgtcttcttc tggagggaca taccgacgtg gtgagcgaag gcgagcggga
     3001 agcgtggacc cacgatccgt tcgccgcgga cctggcggag ggcaggatct acggccgcgg
     3061 cgcctgcgac atgaaggcgg gcctggcagt caacctcctc acggcggcag ccttctgccg
     3121 ggctggggct tcctgggcgg gccggatccg gttggccctc gtctgtgacg aagaaggcat
     3181 gatgagcggg atcaagcact tcatccgctc cggtcacgcg gacggcgtgg acgcctgcct
     3241 ggtgccggag ccggaggaga accagctctg catccgcatg aaaggcgcca tccgggccgt
     3301 cgttcgggtg cacggcaaga tggcccacgg ggccatgccg ctcaccggta tcaacccaaa
     3361 cccgaggctt gcgcggatca tcctggcgtt cgagcgattc gaagcggagg agaaggagcg
     3421 ctgcggagcg gatccctatc tcgggcttcc atcggtcacc tttaccgtcc tgcaggctcc
     3481 gccgtcaggc gtgccggccc agctcaacgt gatgccagga gaggcggtcg gcttcgtgga
     3541 tatccgcacc accctgagcc aggatcacga gaccgtccga tccggacttc gcgccgtcct
     3601 gcgctccctg gcctccctgg atcccgattt caacgcgcag atcgagttca tcgaggaccg
     3661 accggttgtc gggatcgagc gggacgcccc gattgctgcc atcgctgccg cggcgtacca
     3721 ggatgtcacc ggacgcgagc cgacctggaa cggtgtgccc ggcgccacgg acggcacgtt
     3781 tctgagcgcg tggaaacgga tcccctgtct ggtcaacggc ccgggctctc ggctcatccc
     3841 tcaccaggcg gatgaatacg ttgacgcgga tcaggcgctg gagtgcgccc gcatctacgt
     3901 cctgacggcc agccgttttc tggcggcttc gggacatccg gcggcctcgg gaagcgcggt
     3961 ggcgtcggta tgagcggagc gccggtgctg gaggtcaagg gcatccgaaa ggcgttcgga
     4021 gccatcaagg ccctgaacgg ggtggacatc acggtcgcgg ccggtgccat ccacgggatc
     4081 atcgggccaa acggctccgg caaaacaacg ctcttcaact gcatcaccgg gctgttgagg
     4141 gcggacgcgg gctccgtacg cctggatggg cgagacatca ccagtcttcc atctcatgcc
     4201 attgccaacg gcggaatccg aaggaccttt caggcgggca agctggtgcc cggcatgacc
     4261 gtgctggaaa acgtcatgtc cgggatcaac gatcggatcc gggtgtcggc ggtggacctc
     4321 ctccgtgcgc ccttccgcgc atcgcgccgg gaggagggga tccgcgagcg cgcgcggcga
     4381 gccctggacc tcgtagggat gcccgaggct gccgagcgct gggcggcgga cctcgtctgg
     4441 acggaacgac agctcgtcca gatcgctcgt gccctgatcg cggaaccaag ggtcctgctg
     4501 ctggatgagc ccgcctccgg aatgggcgtg aaggaagcgg agaaggtgga gcggatcctg
     4561 cggggggccc gcggacgcgg gatgaccgtc gtggtggtca gccacgacgt gaagctgctg
     4621 atgaacctgt cggatctggt gacggtgctc aacttcggcg agaagatcgc cgagggcgaa
     4681 ccttcggtcg tccagaacga ccctcgagtc ctggaggcct accttgggac gcagtgacgg
     4741 acaagcgatg ctggcggtcg aggatctctc cgtttcctac ggccacgtca acgcgcttcg
     4801 cggcgtcacc atcgatgtcc gcccgggtga gatcgtcgtc ctgatcggag caaacggagc
     4861 cggcaagacg acgctcctgg agaccatcct ggggatcaac accccgcttc ggggcaggat
     4921 ccggttcgac ggggaatcca tcggcggcgt ctccgcggac cgcaacgtgc gctccggact
     4981 gtgcctggtg cccgaagggc gtggggtatt tgcctcgatg acggtcctgg acaacctcct
     5041 tctgggcgcc caccacaacc tgcgcaacgt ctcgcggaac ctggatcggg tctacgagtg
     5101 gttcccggtg ctcgaggccc gccgggagca gatcgcccga accctgagcg ggggtgagcg
     5161 ccagatgctg gccataggcc gcgccctgat gtccgcccct cgcctgatca tggtggacga
     5221 gccctccatc gggctggctc ccatcgtggt gaacgacatc ttctccatcc tcacccggct
     5281 caacaaggag ggctacacga tcctcctggc ggagcagaac gcctacaagg cgctcacctg
     5341 cgcccatcgc ggctacgtgc tcgaaacggg ggtcgtcagc ctgtccgggg atgcccgcgc
     5401 cctgctggag gaccctggcg tgaaagccgc ctacctggga gcctgatcgt gcaggtattc
     5461 gtcgcgcagc tcatcaacgg cctgtccctg gggagcatct acgtgctcct ggtcaccggc
     5521 ttcaacctgc tgctgctggt agccatgatc attcacttca cctatccgca ggtggtcgtg
     5581 ttctccatgt acatcacctg ggtggtcctt cgggccacgg ggaacaacct tccgctgggc
     5641 atcctggccg gcgtcgcatc ctccatcgcg atgaacctgg ccagcgcccc gtttttccag
     5701 aaggtcatgc agaaacgggg agcggtggac atcaacagca ccatggtcct ttccatgggc
     5761 atcgggatga tcatcacgga cgtgctctcc cactcgttca accagggtct gccgatagcg
     5821 tttcccacga gcctgtcgag cgacactccc ctggcgcgct tcggggtcat cagcattgcc
     5881 accgggcagg tgtgggcgct gggcgtgggg atcgttgccg tgacggcggt cttcatcctg
     5941 ctgtaccgca gccggctggg aagggc
//
LOCUS       DASRZS010000507         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4510315, whole genome shotgun sequence.
ACCESSION   DASRZS010000507 DASRZS010000000
VERSION     DASRZS010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4510315"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VFH83_12400"
     CDS             complement(<1..534)
                     /locus_tag="VFH83_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6487218.1"
                     /translation="MIHQPTKPAERRRTRKADENAVSLFLNEIVRIPLLSREEEERLA
                     RRAARGERPAKESLIRANLRFVLSVARRYQNQGLPLEDLISEGTIGLMNAVEHFDVEK
                     GYHFISYAVWWIRRSILKAICETSRTIRLPVGRANELVQIEKVRKDLQGEQFVASETD
                     MIAKKLDLDREHVSDLMN"
     gene            1122..>2159
                     /locus_tag="VFH83_12405"
     CDS             1122..>2159
                     /locus_tag="VFH83_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET6487219.1"
                     /translation="MRSRLAPMGGTYAVFLLCVAVTAALCGCSPRRDGSSGGASAPGA
                     IGVGGPTAPTATPDAATPDAAAPDGAPGAAPDDRPTAGPGGEEPRASGAFGIGAMVVS
                     VRVTSVIDGRLTAELKTSGTVCPVMQSQVVGQTSGVVSRVLHRAGDWVQGGDIVVQLD
                     DTQLTLSLKTAQAALSSAQITLATKQGVTAQAAPKLQLAVKSAESALASATRTCQSQK
                     TLFDMGSVTAADLDKAQSALASAQATLEGAKMDLDQNEKAGVQDLAQLELAADQAGYQ
                     VQQAELNLRNAAIRAPFDGVLVAIRLNPGEYANGNTPAFTIASRERELDFTVPPSEAA
                     SLPVGAEVRFDL"
BASE COUNT          373 a          731 c          690 g          365 t
ORIGIN      
        1 gttcatcagg tcggagacgt gctcgcggtc caggtcgagc ttcttcgcga tcatgtctgt
       61 ctcggaggcc acaaactgct ccccctggag gtccttcctg accttctcga tctggaccag
      121 ctcgttcgct cgaccgaccg gcaaacggat ggtgcgggat gtttcgcaaa tggccttcag
      181 gatggaccgg cggatccacc ataccgcata cgagatgaag tggtacccct tctccacgtc
      241 gaagtgctcg acggcgttca tcagcccgat ggttccttcg ctgatgaggt cctccaacgg
      301 caagccctgg ttctgatacc gccttgccac gctgagcaca aagcggaggt tggcccggat
      361 cagactctcc ttggcgggcc gctcgccccg ggcggcccgc cgcgccagac gctcttcctc
      421 ctcccgggag agcaagggga tccggacgat ctcgttcaga aagagagaaa ccgcgttctc
      481 atccgctttg cgggtcctgc gcctttcggc gggttttgtg ggctgatgga tcatgagcct
      541 cccgttgccg tcctctccag cttcgttccg tccgtgttac ccgggggcgt ctcagaacag
      601 acccgcgacg accccgatca agctcagcac ggtggagaag gaccccagcg ggcgagctca
      661 gcgggcgtcc gcacctcgcc ggcagcaaac gcctcggtgg ccatcggcac caccacgtcg
      721 accgtcacgc gttccctcgt gcttccgcgg gtgagcgtgg cgtgctccgc cccagcgaaa
      781 gggtctggac ctctcagcgc ctcaagggcc atccgcggcg cgaagggtcg gttcctgttc
      841 gcatcgcgtg cgcggaggaa actaggaatc gccccgggtc acggatggcc gtcgtactca
      901 tgggacaaga ctacgccgga ggtacagcca gtccatcaac gctctgtaaa atgccggtaa
      961 aagctcgatc aaccggcaac caggccgacg ttattattct tttgcacaca ttttacatcc
     1021 gatcgatctc cccgagggta ggatggatag cgcgatcacc gaggtcgtcg aaagacgtcc
     1081 cgccacccgc gcgctcgggt ccaaagatag aggagaatca gatgcggtcc agattggctc
     1141 ccatgggcgg tacgtacgcg gtgtttctgc tctgcgtcgc ggtgacggcg gccctctgcg
     1201 gatgttcgcc tcgaagggac ggctcgtccg ggggcgcttc ggccccgggc gccatcggtg
     1261 tcggaggccc gactgccccg accgccacgc ccgacgccgc cacgcccgac gccgcagcac
     1321 ccgacggcgc gcccggtgcg gcgccggatg acaggcccac cgccggaccc ggcggtgagg
     1381 agcctcgcgc ctcgggcgcc ttcgggatcg gtgcgatggt cgtttcggtg cgggtgacct
     1441 ccgtcatcga cggtcgtttg accgcggagc tgaagacatc cggcaccgtc tgcccggtga
     1501 tgcagagcca ggtggtggga cagacctctg gagtcgtctc tcgcgtgctg caccgcgccg
     1561 gagactgggt ccagggcggt gatatcgtgg tgcagctcga cgatacccag ctcacgctat
     1621 ccctgaagac agcacaggcc gcgttatcca gcgcgcagat caccctcgcc acgaagcaag
     1681 gggtcacggc ccaggccgcc cccaagctgc agctcgcggt gaaatccgcc gagtccgcgc
     1741 tggcctcggc cacccgcacc tgccagtccc agaagacgct cttcgacatg gggagcgtaa
     1801 ccgcggcgga cctggacaag gcgcagagcg cccttgcatc ggcccaggcg actctggaag
     1861 gcgcgaagat ggacctggat cagaacgaga aggcgggggt ccaggacctg gcccagctcg
     1921 agctagccgc ggaccaggcc ggctaccagg tccagcaggc agagctcaac ctaagaaacg
     1981 ccgccatccg ggccccgttt gatggggtcc tcgtggccat caggctcaac ccgggcgaat
     2041 acgcgaatgg gaacactccg gccttcacga ttgccagtcg ggagcgggag ctggatttca
     2101 ccgtccctcc ctcggaggcg gcaagcctcc ccgtgggcgc ggaggtccga ttcgacctc
//
LOCUS       DASRZS010000508         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1821116, whole genome shotgun sequence.
ACCESSION   DASRZS010000508 DASRZS010000000
VERSION     DASRZS010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821116"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFH83_12410"
     CDS             complement(<1..438)
                     /locus_tag="VFH83_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET6487220.1"
                     /translation="MTKYREPWRRIVAEALRQVASQSGASAPAAEDVVMETPPKPELG
                     DIGFPMFPFARVLRKAPAAIAQDVARLASQAPAAAGLGEARAEGPYVNVRLAPAPVID
                     DVLKTVAAEGEAFGRTKDLAGQKVMLEFSCPNTNKPLHLGHLRN"
     gene            554..1525
                     /locus_tag="VFH83_12415"
     CDS             554..1525
                     /locus_tag="VFH83_12415"
                     /inference="COORDINATES: protein motif:HMM:NF044299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487221.1"
                     /translation="MEIISRMSIAPFLRTAAPDAPRVARGLLLAVAAAVALASCATVP
                     QRTQSQWLGALPQDATMYASLSVPHSLAFLQKTLAQAGPDYKDVGTLMERTSRMVAAV
                     TLQEGQAARFSLLALGNYPAGIIGMRLSGSKDWHREASGRSQYWQSSKIGVQVSVPNN
                     SILLAANGDIVPLLGRYADPYPVTMPPDVSSDMESMDVVVYLPRLPGALSHTSSSGMA
                     IPIHEVWVDAAKASGGYALTGTANTESEKDARLFVLVLRLGIVSWMKTQNVPNASERL
                     KTISVVSSANQVKLSGLSLSEDELLSVLRSLLKGASPPPEAEAQASE"
     gene            1522..2172
                     /gene="hisH"
                     /locus_tag="VFH83_12420"
     CDS             1522..2172
                     /gene="hisH"
                     /locus_tag="VFH83_12420"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HET6487222.1"
                     /translation="MSARPIVVVDYGAGNLTSVEHVLRHLGAPFLTTARPEDLRDAEG
                     VIFPGVGEAASSMAELSRTGLDEALRRFVASGRKVLGICIGCQVIFDRSEERDTTCLG
                     IVGGVVRRFPRYNGLKVPHMGWNAVHVEQAHPLLRGIPQDSAFYFVHSFYPEPVDARM
                     VVATTDYGFPFASVVATGNLAACQFHVEKSGGVGLALISNFLAWDGRWEGPCSPGD"
     gene            2154..>2688
                     /locus_tag="VFH83_12425"
     CDS             2154..>2688
                     /locus_tag="VFH83_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HET6487223.1"
                     /translation="MLSRRLIVCLDVRDGRTTKGIQFRDNVDVGDPVEMARRYYQDGV
                     DELVFYDITASAERRRIRIDTVRRVAEQVFIPFSVGGGISTAEEMREVLLAGAEKVSV
                     NSAAVRNPQLITQGAALYGTQCVVLAMDVERDPSRPCGYGVVIDGGRKKTGLDALEWA
                     RKAEELGAGEIVLNSIDA"
BASE COUNT          423 a          906 c          904 g          455 t
ORIGIN      
        1 gttcctcaag tgacccaggt gaagaggctt gttggtgttc gggcaggaga actccagcat
       61 gaccttctgg ccggcaaggt ccttcgtccg gccgaatgct tcgccctccg ccgctaccgt
      121 cttcagcacg tcgtcgatca cgggggcggg ggccagccgc acgttgacat acgggccctc
      181 ggcgcgcgcc tctccgagcc cggcggccgc gggcgcctgg gacgccaggc gcgccacgtc
      241 ctgggctatc gcggccggcg ccttgcggag cacacgggca aacgggaaca tggggaagcc
      301 gatgtcgccg agctccggct tcggcggggt ctccatcact acgtcttccg ccgccggcgc
      361 ggaagcgccg ctctgggagg ccacctgtcg gagcgcctcc gcgacgatcc tgcgccacgg
      421 ttcccggtac ttcgtcatgg tgtcccgtat ttaaccagag cggtccggcg gtttcaaggc
      481 ccgcgggtca cgccgagcct gaccggccat ggttcgcctt gaccggacag cccctttttc
      541 cgtataccgt gccatggaga tcatcagccg aatgtcgatc gctcctttcc tgagaacggc
      601 cgccccggac gcgccgcggg tcgcccgagg tctgctcctg gccgttgcgg ctgcggttgc
      661 cctggcatcc tgcgccaccg tgcctcagcg cacgcaaagc caatggctcg gggcgctgcc
      721 tcaggacgca acgatgtacg cctccctctc cgtgcctcat tcgctggcct tcctccagaa
      781 gacgctcgcc caggcgggtc cggactacaa ggacgtgggg accctgatgg agcgaaccag
      841 ccggatggtc gcggcggtga ccctccagga gggccaggcc gcccggttct ccctcctggc
      901 gctcggcaac tacccggcgg gtatcatcgg gatgcgcctt tccggctcca aggactggca
      961 ccgggaggcc agcggccgtt cccagtactg gcagtccagc aagatcgggg tccaggtgag
     1021 cgttccgaac aactcgatcc tcctggccgc aaacggcgac atcgtccctc tcctggggcg
     1081 atatgcggac ccgtatccgg tcaccatgcc gcctgacgtt tcttcggaca tggagtccat
     1141 ggatgtcgtg gtctatctgc ccaggctgcc gggggcgctg tcccacacgt cgagcagcgg
     1201 aatggcgatc cccatccacg aggtctgggt ggacgccgcc aaggcttccg gcggctatgc
     1261 gctcacgggg accgcgaata ccgagtcgga gaaggatgcc aggctcttcg tcctggtgct
     1321 gaggctcggg atcgtgagct ggatgaagac ccagaacgta cccaacgcct cggaacggtt
     1381 gaagacgatc tccgtcgtct ccagcgccaa ccaggtgaag ctctccggcc tgtccctttc
     1441 ggaggacgag ctgctgtcgg tcctgcggtc gctcctgaaa ggggcctccc cgccgccgga
     1501 ggcggaagcg caggcctcag aatgagcgcc cgacccatcg tcgtcgtcga ctacggcgcc
     1561 ggaaacctga ccagcgtgga gcacgtgctt cggcacctcg gcgccccgtt cctgaccacg
     1621 gcccgacccg aggacctgcg ggacgcggaa ggcgtgatct tccccggcgt tggcgaggcc
     1681 gcctcttcca tggcggagct ctcacggacc gggctggatg aagccctcag gagattcgta
     1741 gcctccggtc ggaaggtgct cgggatctgc atcggctgcc aggtgatctt cgatcggtcc
     1801 gaggagcggg acaccacctg cctggggatc gtgggcggag tcgttcggcg tttcccgagg
     1861 tacaacggcc tcaaggtccc gcacatgggc tggaacgcgg tccacgtcga gcaggctcat
     1921 cccctcctgc gggggatacc ccaggacagc gccttctact tcgtgcactc cttctaccct
     1981 gagcccgtcg acgcgcggat ggtggtcgca accaccgact atggcttccc gttcgcctcc
     2041 gtcgtggcga ccggcaacct cgccgcctgc cagttccacg tcgagaagtc tggcggcgtg
     2101 gggcttgcgc tgatctcgaa ctttctggcc tgggacggcc gttgggaggg accatgctct
     2161 ccaggcgact gatcgtgtgc ctcgacgtcc gggacggccg tacgaccaaa gggatccagt
     2221 tccgcgacaa cgtggacgtc ggagaccccg tggagatggc ccggcggtac taccaggacg
     2281 gggtcgatga gctggtattc tacgacatca ccgcctcggc ggagcggcgc aggatccgca
     2341 tcgacacggt acgacgggtc gccgagcagg tgttcatccc gttctccgtg ggcggcggca
     2401 tttcgaccgc ggaggagatg cgggaagtcc tcctggcggg ggcggagaag gtcagcgtga
     2461 actccgccgc tgtccgaaac ccccagctca tcacgcaggg ggccgccctc tacggcacgc
     2521 agtgcgtggt cctggccatg gacgtggagc gggacccgag caggccgtgc ggctacggag
     2581 tcgtgatcga cggcggcaga aagaagaccg gcctggacgc cctggagtgg gcacggaagg
     2641 ccgaggagct aggggcaggt gagatcgtgc tcaactccat cgacgcgg
//
LOCUS       DASRZS010000509         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2977537, whole genome shotgun sequence.
ACCESSION   DASRZS010000509 DASRZS010000000
VERSION     DASRZS010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2977537"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VFH83_12430"
     CDS             <1..533
                     /locus_tag="VFH83_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6487224.1"
                     /translation="VHRLMRVLCDFNLAVLRRFAAYPIDGVYFGDDWGSQRAMLMAPA
                     TWRRLVKPYLQEMYGQAHRQGWDVFIHCCGQVTPILDDLVEIGLNVFNPFQPEVMDIE
                     SLMKRYSRRLAFYGSLSIQRTLPFGTEDDVRREVRHRLSLARAHGGLILSPSHDMPPD
                     VPVKNVLAMLGALRDQ"
     gene            660..1421
                     /locus_tag="VFH83_12435"
     CDS             660..1421
                     /locus_tag="VFH83_12435"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET6487225.1"
                     /translation="MFIEERLTRILRLLKEENRVTVREVSENLQVSEDTIRRDFSRLA
                     ERGLVLRTHGGIMSRESVALDPGMHEKSVQHRDEKEAIARKAADLVAHGEIIVLDAGT
                     TTERVVKHLAEKQNLTILTDALNIAMEATRRSIPTVILGGIIRNSTLCITGPDAVEMI
                     RHYHTDKLIMGVSAISTSRGLMTANRMEAEIKKELIKTASQVIVVADHSKLQMPGFYT
                     FASLKDLSILVTDKGANPDLVKELQDGGVRVLLAD"
     gene            1472..1864
                     /locus_tag="VFH83_12440"
     CDS             1472..1864
                     /locus_tag="VFH83_12440"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6487226.1"
                     /translation="MSDDQLRTTVQTSAERPAFGAQLGTKAFLVVVSSRQFGLLQVIG
                     APPLKIGRSRECKLRLDDAAVSTVHCTVTQDPRKGWILEDAGSTNGTRLNGRRIEAPV
                     ALSYGDRISVGSTILRFYIEEVPEGASA"
     gene            complement(1939..2550)
                     /locus_tag="VFH83_12445"
     CDS             complement(1939..2550)
                     /locus_tag="VFH83_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487227.1"
                     /translation="MKRSGSRILRVTALAVFSVVLAAAAHGQLVISAGANAYAASSGL
                     MAGGGLEAEDWLSVSGDNASRGLMIGLGLQYVALTTSVTHTPGAGWIMPVTLKYGFPV
                     GRDLVLGLGAGAALVIADVDQEDYFGDYYVTSSVGGAPFVTAGLYYFFSGRLNLQAVA
                     LAGALFNDAGTVPFLGLQVLVGYYIELPHPARTRDELDDPDDY"
BASE COUNT          487 a          873 c          860 g          397 t
ORIGIN      
        1 ctgttcaccg gctgatgcgc gtgctgtgcg atttcaacct ggcggtcctg cggaggttcg
       61 ccgcctatcc catagacggc gtgtacttcg gcgacgactg gggatcgcag agggccatgc
      121 tcatggcgcc cgcgacgtgg agacgcctgg tcaagccgta cctgcaggag atgtacggcc
      181 aggcgcaccg gcagggctgg gacgtattca tccactgctg cggccaggtc acgccgatcc
      241 tggacgacct ggtcgaaatc ggactgaacg tcttcaaccc gttccagccg gaagtgatgg
      301 acatcgagag cctcatgaag cgatactctc ggcgcctggc attctacggc agcctgagca
      361 tccagaggac gctgccattc gggaccgagg acgacgtgcg gcgcgaggtc cgacaccggc
      421 tttcgctggc aagggcccat ggtggcctga tcctgtcccc gtcccatgac atgccgccgg
      481 acgtccccgt gaagaacgtc ctggccatgc tcggcgcgct gagggaccag tgagcgcgcg
      541 cgtgggaacc ggcacttgcc atttccgagg cgttgcggtt ctctttggta tctgcccgcc
      601 cgctcgccgg caggcggacg ggcacgtggg cgggcacgcg ggataaaaga gatcgagcgg
      661 tgttcatcga ggaacgactg accaggattc tccggctgct gaaggaagaa aaccgggtca
      721 ccgtgaggga agtcagcgag aacctccagg tgtcggagga caccatccgg cgggacttca
      781 gccggctggc ggaacgaggc ctggtcctgc gcacccacgg cggcatcatg tcccgggaga
      841 gcgttgcgct cgaccccggg atgcacgaga agtcggtgca gcaccgggat gaaaaggaag
      901 ccatcgcccg gaaggccgcg gacctcgtcg cccacggcga gatcatcgtg ctggacgccg
      961 gcaccacgac cgagcgggtc gtcaagcacc tcgcggaaaa gcagaacctg accatcctca
     1021 ccgatgcgct gaacatcgcg atggaagcga cccgtcgatc catccccacg gtcatcctgg
     1081 gcggcatcat ccgcaactcc accctgtgca tcacgggacc ggatgcggtg gagatgattc
     1141 gccactatca caccgacaag ctcatcatgg gcgtgagcgc aatctccacc tcccgggggc
     1201 tgatgaccgc caaccgcatg gaagcggaga tcaagaagga gctcatcaag accgccagcc
     1261 aggtcatcgt ggtggcagac cattccaagc tgcagatgcc cggcttctac accttcgcgt
     1321 cgctcaagga cctttccatc ctggtcacgg acaagggcgc gaatcccgac ctggtaaagg
     1381 agctgcagga cggcggcgtc agggtccttc tcgccgacta gtttccgacc ttctgttcca
     1441 gcaagcgcaa caccgaggag gagccagcgc gatgagtgat gaccagcttc gaaccaccgt
     1501 gcagaccagc gccgaacggc ccgcgttcgg ggcccagctc gggaccaagg cgttcctggt
     1561 ggtcgtatcc tcccggcagt tcggcctgct gcaggtgatc ggcgcgccgc cgctcaagat
     1621 cgggagaagc agggagtgca agctccgcct ggatgatgcc gcggtcagca ccgtgcactg
     1681 cacggttacc caggacccga ggaagggctg gatcctggaa gacgccggct ccaccaacgg
     1741 cacccggctg aacgggcgtc gcatagaggc gcccgtggcg ctgagctacg gtgaccgcat
     1801 cagcgtcggg tccacgatcc ttcggttcta catcgaggag gtgccggagg gcgcctcggc
     1861 ctgatccgcg cgctgcggac gggctcgcgg cagagccgcg gcctccgcgc ccgcggcagc
     1921 cagtaaccgc tccagcgctc agtagtcgtc agggtcgtcc agctcgtccc gtgtgcgcgc
     1981 cgggtgcgga agctcgatgt agtaccccac gagcacctgc aggccgagaa acggcaccgt
     2041 gcccgcgtcg ttgaacaggg cccctgccag cgccaccgcc tgcaggttga gacggccgga
     2101 gaagaagtag tacaggccag ccgtcacgaa gggcgcgccg cccacggagg acgtgacgta
     2161 gtagtcgccg aagtagtcct cctggtccac gtccgcaatg accagcgcgg cgcccgcccc
     2221 cagccccagg accaggtctc tccccacggg gaagccgtac ttgagcgtca ccggcatgat
     2281 ccagccggct cccggggtgt gcgtgaccga cgtggtcagg gcgacgtact gcaggccaag
     2341 gccgatcatc agcccacggg aggcgttgtc cccggagacg ctgagccagt cctctgcctc
     2401 cagcccgccc cccgccatca gcccggacga agccgcgtag gcgtttgcgc cggcggatat
     2461 gacgagctgc ccgtgagcgg cagcggccag gacaacggag aataccgcca gcgcggtgac
     2521 ccgcaggatc cgacttccgc ttcttttcat gatgggactc cagtcattct accacggcgt
     2581 cctcgcgcgg ggcagggtcc accgcgcaag ggcgctc
//
LOCUS       DASRZS010000510         3787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1060909, whole genome shotgun sequence.
ACCESSION   DASRZS010000510 DASRZS010000000
VERSION     DASRZS010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060909"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VFH83_12450"
     CDS             complement(<1..217)
                     /locus_tag="VFH83_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6487228.1"
                     /translation="MSESLRVGIIGLGRAGEKHARAFSRIPGVTVGALCDASRERLGP
                     LTASLGARAYTDYRELLADPNVDAVSIV"
     gene            complement(236..1048)
                     /locus_tag="VFH83_12455"
     CDS             complement(236..1048)
                     /locus_tag="VFH83_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6487229.1"
                     /translation="MRSKLPHLPLLLLSVFFFLPFLWMVSTSLKSLPEVFSLPPHLIP
                     RVFQWQNYQLAFDRAPFLVYTRNTLFYSVMVVVGFLFSSPLVAYSVSRIDWRGGKILF
                     PIILSTMLLPYPVTMIPLFIVFQRLHWVGGFAPLIIPAFTGGAFYIFLMRQFFLTVPG
                     SLLDAARIDGASEVRIYASIMLPLCAPALTSVAIFAFLGAWSDFLGPLVYLTRQLSYT
                     LSLGLHAFMASHTVEWHYLMAISVLFTVPIVIIFFLSQRYFIEGIATTGMKV"
     gene            complement(1083..2000)
                     /locus_tag="VFH83_12460"
     CDS             complement(1083..2000)
                     /locus_tag="VFH83_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006208672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6487230.1"
                     /translation="MKRPRRNRRRNALLGLLFASPWIIGFLCFTIYPIASSFYYSLTN
                     FNIFQHPKYVGFANYLQLFSDESFYKGLYNTFYMTIVGTPVYLVFALLAALLLNGKLK
                     TISVYRTIFYLPSIVPLVASSLLWIWILNPTNGLLNNILKALHLYQPNWLGNPALTKP
                     SLILIGMWATGQTMVIFLAALKDVPRYLHEAAVIDGAGTWSRFLHVTLPSISPVILFQ
                     VIMSVIYSFQYFTQAYVIAGSQGGLNVASGGPENSMLFYVMYLFHNAFYFLKMGKASA
                     MAWILFVIIALVTWVVFRTSRQWVSYGNE"
     gene            complement(2097..3359)
                     /locus_tag="VFH83_12465"
     CDS             complement(2097..3359)
                     /locus_tag="VFH83_12465"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6487231.1"
                     /translation="MRRSLIIAVLCLTGAVLGFTAPTQVQYWYLWGGNESQLMKSMVQ
                     KFNASQTQYQVTPLEVPDMQKILVAISSGSGPDVTDDFASNVAGYASKGILEPLDPYI
                     ARTQYGTSDFVPSALQTCQYQGTTYALPLNMNFFMLFYNKALFRQAGIAAAPKTDKEL
                     LDDAIKLTKVNSDGTLDTLGFPDFPIVYYLNNMTFAFGGRYLSPDGKTLTPDNAGTLR
                     ALNLIVSYRQKFGVDPVAKFSTAGGYLQPTDPFIAGKQAMRIDGPWFGNVVRNQLKMT
                     TAQLDYGVAPLPYPDGHPELALGGQVDSSILYIPASAANKDGAWAFLSWMLNDKNMID
                     VDAGMGNFPSRMSALGADAFAKTPDFQAFASLVKSSKNLGVFPAFAGQAEFGKLLSDQ
                     AEKVWNLKMSATDAMAEAKAKAQALLNQ"
     gene            complement(3441..>3787)
                     /locus_tag="VFH83_12470"
     CDS             complement(3441..>3787)
                     /locus_tag="VFH83_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase"
                     /protein_id="HET6487232.1"
                     /translation="FRDIGIPLTVPDSPTHLMLHLAAKLVLNTLSTATMGKLGRILGN
                     WMIQVDATNKKLVDRATRIVSELAGIGYEEACIELHRTLATVPASGGREQVEDSCVVR
                     TLRRLGRLPRTG"
BASE COUNT          779 a         1065 c         1295 g          648 t
ORIGIN      
        1 ggacgatgct gaccgcatcg acgttcggat ccgccaggag ctcgcggtag tcggtgtagg
       61 cgcgcgctcc cagggaagcg gtcaaggggc ccaggcgctc gcggctggcg tcacacagcg
      121 cgcccacggt tacgccgggg atgcgggaga aggcccgcgc gtgtttctct ccggcccgac
      181 ccagtccgat gattccgacc ctcaacgact ccgacacggt tgcctcccga gtgcgttata
      241 ccttcatgcc ggtcgtggcg atcccctcga tgaagtagcg ctgggacagg aagaagatga
      301 tcacgatggg aacggtgaag agcacggaga tcgccatgag atagtgccat tccacggtat
      361 gggacgccat gaaggcgtgc agtcccaggg aaagggtgta gctgagctgc cgcgtcaggt
      421 agaccagcgg tcccaggaag tccgaccacg ccccgaggaa cgcgaagatc gcgaccgacg
      481 tgagcgcggg cgcgcaaagc gggagcatga tgctcgcgta gatgcgcacc tcggaggctc
      541 catcgatgcg cgccgcgtcc aggagcgagc ccgggacggt gaggaagaac tggcgcatca
      601 ggaagatgta gaacgcgccc ccggtgaacg cgggaatgat caagggcgcg aagcccccta
      661 cccaatgcag cctctggaac acgatgaaca gtgggatcat ggtcaccggg tacggcagga
      721 gcatggtgga gaggatgatc gggaacagga tcttgcctcc cctccagtcg atccgggaaa
      781 cggagtacgc gaccaacggg gaggagaaca ggaacccgac gactaccatc accgaataga
      841 acagcgtgtt gcgggtatag acgaggaaag gcgcccggtc gaaagcaagc tggtagttct
      901 gccactggaa gacgcgcggg atgaggtgag gcggcaggga gaagacctcc ggaagagact
      961 tcagggaggt cgagaccatc cagaggaacg gcaggaagaa aaacacgctc aagaggagca
     1021 gaggcagatg aggcagcttg gacctcacga ggcgccccag cctccggcgc gggaccggga
     1081 tctcattcgt ttccatagct cacccactgc cgggacgtcc ggaacacgac ccacgtcacc
     1141 agggcgatga tgacgaagag gatccaggcc atggccgatg ccttccccat cttcaggaaa
     1201 tagaaggcgt tgtggaaaag gtacatgacg tagaagagca tggagttctc ggggccgccg
     1261 ctggcaacgt tgaggccgcc ctgggacccg gcgatgacgt aagcctgggt gaagtactgg
     1321 aagctgtaga tcacgctcat gatgacctgg aagaggatca cgggggaaat actcggcagc
     1381 gtgacgtgga ggaaccggga ccaggtcccg gccccgtcga tcacggcggc ctcatggagg
     1441 tagcgcggca cgtccttgag ggcggccagg aagatcacca tggtctgtcc ggtggcccac
     1501 atcccgatca agatgaggga aggcttggtc agggcgggat tgcccagcca gttgggctga
     1561 tacaggtgga gcgccttgag gatgttgttc aacagcccgt tggtagggtt gaggatccag
     1621 atccacagaa gggaggaggc gacgaggggc acgatggacg gcaggtagaa aatggtgcgg
     1681 tagacggaga tcgtcttcag cttgccgttc aggagcaacg ccgcgaggag ggcgaaaacc
     1741 aggtacaccg gcgtgcccac gatcgtcatg tagaaggtgt tgtacaggcc cttgtagaaa
     1801 gactcgtccg agaagagctg gaggtagttg gcgaacccga cgtacttcgg gtgctggaag
     1861 atgttgaagt tcgtgagact gtagtagaac gaggacgcga tcggataaat cgtgaagcag
     1921 aggaagccaa tgatccatgg cgaagcgaag agcaagccca gcagggcatt gcgtcgccgg
     1981 ttccggcgcg gtcgtttcat cgaatctcct ggccaggggt ggttcggccc gccgcaagag
     2041 gcccgggctg actgcccggg tgcccgaagg cgggccgata ccggtaaccg cccgagctac
     2101 tgattcaaga gcgcctgggc cttggccttg gcttccgcca tggcgtccgt ggcgctcatc
     2161 ttcaggttcc agaccttctc ggcctggtcg ctcaagagct tcccgaactc cgcctgtccg
     2221 gcaaacgccg ggaacactcc gaggttcttg ctgcttttga ccagggaggc gaacgcctgg
     2281 aagtccggcg tcttggcgaa cgcgtcggct cccaaggcgc tcatccggct ggggaaatta
     2341 cccatgccgg cgtccacgtc gatcatgttc ttgtcgttca gcatccagct caggaacgcc
     2401 cacgccccgt ccttgttggc ggcgctggcc ggaatgtaga ggatcgagga atccacctga
     2461 ccgccgagcg ccagctcggg atggccgtcg ggatagggca ggggcgcgac gccgtagtcg
     2521 agctgagccg tagtcatctt gagctgattg cggaccacgt tgccgaacca ggggccatcg
     2581 atgcgcatgg cctgcttgcc cgcgatgaac ggatccgtgg gctggaggta accgccagcc
     2641 gtggagaact tggccaccgg atccacgccg aacttctggc ggtacgacac gatgaggttc
     2701 agagcccgta gggttcccgc gttgtccggg gtgagggtct tgccatccgg ggacaggtac
     2761 cttccgccga aggcgaaggt catgttgttc agatagtaga cgatcgggaa gtcggggaat
     2821 ccgagggtgt ccagggttcc gtcgctattg accttggtga gcttgatggc gtcgtcgagg
     2881 agctccttgt ccgtcttcgg ggcggcggca atgccggcct ggcggaacag cgccttgttg
     2941 tagaagagca tgaagaagtt catgttgagc ggcagggcgt aggtcgtgcc ctggtactgg
     3001 cacgtctgaa gggcggacgg gacgaagtcc gaggtgccat actgggtcct ggcgatgtac
     3061 ggatcgaggg gctccaggat ccccttggag gcgtaccccg ccacgttgct cgcgaaatcg
     3121 tccgtgacgt cgggcccgga gccgctggag atcgcgacga ggatcttctg catgtcgggc
     3181 acttcgaggg gagtcacctg atactgggtt tgggacgcgt tgaacttctg gaccatgctt
     3241 ttcatgagct ggctctcgtt cccgccccac aggtaccagt actgcacctg cgtgggagcc
     3301 gtgaaaccca gaacggcccc cgtcaagcac aggaccgcga tgatcaaaga tcttctcatc
     3361 tatccacctc cttggggata acgttgaacc attgcagcgc ttttctgcct ctctgccaag
     3421 acgcctttgc agggggcgca ctaccccgtc cggggcaatc ggcccaggcg gcggagcgtc
     3481 cgcacgacgc aagagtcttc cacctgttcc cggccgccag aagcgggcac ggtggccagg
     3541 gtccgatgca gctcgatgca ggcctcctcg tacccaatcc ccgcgagctc ggatacgatc
     3601 cgagtagctc ggtcgacgag cttcttgttg gtggcatcca cctggatcat ccagttcccc
     3661 aggatgcggc cgagcttccc catggttgcc gtggaaaggg tattgaggac gagcttcgcg
     3721 gccaggtgaa gcatcagatg agtggggcta tcagggacgg tcaggggtat gccgatgtcg
     3781 cggaagc
//
LOCUS       DASRZS010000511         5933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_664922, whole genome shotgun sequence.
ACCESSION   DASRZS010000511 DASRZS010000000
VERSION     DASRZS010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664922"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFH83_12475"
     CDS             complement(<1..443)
                     /locus_tag="VFH83_12475"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HET6487233.1"
                     /translation="MAGATRPWSFSMTAISVALGTLAAGPARIDWVLALVTLIGALCL
                     HAATNLANDLYDYRDGIDKPGSPGVRARHHPLVERTLSPRQVLTGAIAFWIAATLVGV
                     CLAAARGWPVLVLTGIGVFAGLFYSAGPVRLKGRVLGELTAFLMWG"
     gene            complement(550..1428)
                     /locus_tag="VFH83_12480"
     CDS             complement(550..1428)
                     /locus_tag="VFH83_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487234.1"
                     /translation="MSLIKMVLCLALLSTALAPLGAQAAGGEAAPFLQIIPHFEEGFL
                     GVIYHTFQSGTGTTDFNFITQGGQDILFPYQRYSVDVVLAGHHRITALYQPLTLSTST
                     VANGNGTNGGQNVVIGGAVFAPGTPMNITYGFDFWRLSYLYDFSNDPSTILGLGVSIQ
                     IRNASIVFSAVNGSDRFVSQNIGPVPILKVHAAHWFSPSFGLDLDADGFYASSAFFNG
                     ATQPFTGWIWDAALSAKTHFMPGVVAYLTARSIGGGAAGNNAYPTITATTTGPGTYTN
                     NVLATMAVTLGVSVER"
     gene            1540..3216
                     /locus_tag="VFH83_12485"
     CDS             1540..3216
                     /locus_tag="VFH83_12485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET6487235.1"
                     /translation="MTEAKRHSVESQKQSEATARQWLSLVVLGLGLAIIIIDGSLVNV
                     IIPAISKEFAAGIVELQWVNSIYALVFAALIVTFGRIGDQFGRRLLFILGVVVFVAGS
                     ILSGASTSIGMLIGARALQGLGAAMTSPSTLSIISGTFTGRMRGVAFGVWGAIAGAAA
                     ALGPLLGGWLATSASWRWAFYINVPIGAVAVVGSLLVVDESRAEAGKTRVDVPGILLI
                     TVGVGALVLGLIEGQTYGWLAPKNGFAIGSWTWPFAGFSVSLASLVLAVAGIVAFVFW
                     ELGMQARGRQPLFDFSLLGYRSFRFGLLTVSIVALGEFGVIFALSLYLQGVLGYTALA
                     TGLTFLPFAALTLFVAPTAGLLSARFGPKWVVTAGMLFEAVFIFLLGRVLRTDTAQST
                     IILILLGYGVGIGLAIAQLTNIVLSEVPANRLGAGSGANNTLRQVGAALGTAVIGAVL
                     STTLSLSVQTGLKGVSEIPDVAKTAIVASLDRGATFSGGSVGVSGAPSGAEGTPAAVA
                     VEKVIKESFVNAARAATVAASLFVLLGAVSSLLIPTTVRRAEPSRLGRVG"
     gene            3219..3653
                     /locus_tag="VFH83_12490"
     CDS             3219..3653
                     /locus_tag="VFH83_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6487236.1"
                     /translation="MYKKIMVPLDGSTAAEAVLDHVRKIAGEGTEVVLVRIAAKPAYE
                     YTLQEPQLSACLDEDAAGEAGQYLSEVAARLKRSGLDVSTCVLPEQGPAARVLEDYAH
                     KATADLIAISAHGKPGLIGRLLGSVAERIVHHGRIPVLLVHP"
     gene            complement(3702..4175)
                     /locus_tag="VFH83_12495"
     CDS             complement(3702..4175)
                     /locus_tag="VFH83_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487237.1"
                     /translation="MTIIVFYSETGTNARIARTMSDALGLQAREIRSARGHSFFGKVF
                     GSRMGRRFPILSMDTDLSRFESVILCAPVWAGGPACQLMTFVDEAELHGKEVAVVLGC
                     GGLRPERALGIIRGTLEAKGARMVAGETLNVGKLRGEALTVAARDLAGAIAAKLS"
     gene            complement(4182..4979)
                     /locus_tag="VFH83_12500"
     CDS             complement(4182..4979)
                     /locus_tag="VFH83_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487238.1"
                     /translation="MESRIKIVQTSNIVCFLGVLVVNTLAATVGINGRLTGALSDEIP
                     NLFVPAGLTFSIWGVIYILLLLFTIGQARGLFSSSRTSPEGTRQVGWLFVLSSAGNIG
                     WLLLWHFRLVGWSVLAMLLILASLVAIHQRLGTGRTPAGQAERWLFRVPFSVYLGWIT
                     VATIANVTAALVKAGWMGFGMQPQFWAVTVVVVAAVLTGAVLATRRDVAYSLVVLWAL
                     VGIAMKRSADPSSASQAVFVAAVVCGSVVAAAVIGSVVRSAVKPLEA"
     gene            5092..>5933
                     /locus_tag="VFH83_12505"
     CDS             5092..>5933
                     /locus_tag="VFH83_12505"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET6487239.1"
                     /translation="MVAMQTKGAFTASLTEHKREISAILDAFFAARRRDLSEVNQLAN
                     EACDRLRQFVLAGKMIRGGLVTVGLSLCRDGPSSRELHMAHQVGAAIELFQSGLLIHD
                     DIMDRDLMRRGRATLFEQYSRDARQGGVNDWRHLGEALGTCVGDVAFFLGYELLGGLS
                     PSPAALGRIIALFSRELAAVGIAQMQDVAWGADPSAQPGTDDILRLYRFKTGRYSFSL
                     PLAAGAILAGAADSSIEALEALGECVGVIFQIRDDELGLWGDEKKTGKPVGSDIREGK
                     KTLL"
BASE COUNT         1083 a         1883 c         1915 g         1052 t
ORIGIN      
        1 ccccacatca gaaatgccgt aagctctccg agcacgcgac ccttgagacg cacaggtcct
       61 gccgaataga acaacccagc gaacactcca atcccggtca gcaccaggac cggccagccc
      121 cgggcagcag ccaagcagac cccaacaagg gtggcagcga tccagaacgc gatggcgccc
      181 gtgagcactt gtctggggga cagggtcctc tccaccaggg gatggtgccg ggcgcgcacg
      241 cccggagagc ccggcttgtc gataccgtcc cgatagtcgt agaggtcatt cgccagattc
      301 gtggctgcgt gcaggcacag cgcgccgatc agcgtcacca gtgcaaggac ccagtcgatc
      361 cttgcaggac cggcagcgag cgtacccaaa gcaaccgaga tcgccgtcat cgagaacgac
      421 cacggcctcg tcgcgcctgc cacccgagcg gcggattgaa gcccggaggc aaagacgggc
      481 gatactccct cttgccttcc tttcatggtt cctcgatacc agcaagtgtg tcctcgaggc
      541 cgtcccgcgt caacgctcca cggaaacgcc aagggtcacc gccatggtgg ccagcacgtt
      601 gttcgtgtag gttccgggcc cggtcgtggt cgcggtgatc gtgggataag cgttgttgcc
      661 tgccgcgcct ccaccgatcg accgcgccgt cagatatgca acgacgccgg gcatgaagtg
      721 cgtcttggcg ctcagcgcgg cgtcccagat ccatcccgtg aacggctgcg tggccccgtt
      781 gaagaaggcg ctggatgcgt agaagccgtc cgcatccaga tccagaccga acgacgggga
      841 gaaccagtgg gccgcgtgga ctttcaggat cggaaccgga ccgatgttct gggagacgaa
      901 ccgatcactt ccgttgaccg cggagaagac gatcgaggca ttccgtatct ggatgctcac
      961 gcccagtccg aggatggtcg acggatcgtt cgagaaatcg tagaggtagc tcaagcgcca
     1021 gaaatcgaac ccgtaggtga tgttcatggg ggtcccgggg gcgaacacgg ctcccccgat
     1081 gaccacgttc tggccgccgt tggtgccgtt gccgttcgcc acggtgctcg tggaaagcgt
     1141 cagcggctgg tacagcgcgg tgatccgatg atgacctgcc aggaccacgt ccacggagta
     1201 gcgctggtag ggaaagagga tatcctgtcc gccctgggtg atgaaattga agtccgtggt
     1261 ccccgtcccg gactggaacg tgtggtagat gaccccgagg aaaccctcct cgaaatgcgg
     1321 gatgatctga agaaacggcg ccgcttcccc accggcggcc tgggcgccaa ggggcgccag
     1381 cgccgtgctg agaagagcca ggcaaagaac cattttgata agagacatcc ttcgagacat
     1441 acttggtacc tcctagtgag ttagagtacc tgaatttcag tggtttggca acacaaaatg
     1501 tggttgacaa tcctgccttc caagggcata gttcagggca tgacggaagc aaagagacac
     1561 tccgtggaga gtcagaaaca atccgaggct accgcccggc agtggctctc cctggtcgtt
     1621 ctgggacttg ggctggccat catcatcatc gacggaagcc tggtgaacgt catcatcccc
     1681 gccatcagca aggagttcgc cgctgggatc gtcgagctgc agtgggtgaa ctcgatctat
     1741 gccctggtgt tcgccgcgtt gatcgtcacc ttcggcagga tcggcgacca gttcggcagg
     1801 aggctgttgt tcatcctcgg cgtggtcgtg ttcgtggccg gctcgatcct gtcaggcgca
     1861 tcgacctcca tcggcatgct gatcggtgcg cgcgcgctcc aggggttggg ggccgccatg
     1921 acatcgccct caacactctc catcatctcc ggcacgttca ccggccgaat gcgcggggtg
     1981 gcgttcggcg tgtggggcgc gatcgcgggc gccgcggcgg ccctgggtcc gctgctgggc
     2041 gggtggctgg ccacctcggc atcctggcga tgggcgttct acatcaacgt gccgatcggc
     2101 gcggtggcgg tcgtgggttc cctgctggtg gtggacgaat cgcgagccga ggccggaaag
     2161 actcgggtgg atgtgccggg catccttctg atcacggtgg gcgtcggcgc cctggttctg
     2221 ggactgatcg agggccagac gtacggatgg cttgctccga agaacgggtt cgcgatcggg
     2281 tcctggacct ggccctttgc cggcttctcg gtctccctgg ccagcttggt gctggccgtg
     2341 gcgggaatcg tagcgttcgt cttctgggag ctcgggatgc aggcgcgggg gcggcagccg
     2401 ctgttcgact tctccctgct ggggtaccgc agcttccggt tcggtttgct gaccgtatcc
     2461 atcgtcgcct tgggggagtt cggcgtcatc ttcgcgctgt ccctctacct gcagggcgtc
     2521 ctgggttaca cggcgctcgc aacggggctt acctttctcc cctttgccgc cctgaccctg
     2581 ttcgttgctc ctaccgcggg cctcctgtcc gcgcggttcg ggccaaagtg ggtcgtgacg
     2641 gcgggaatgc tcttcgaagc cgtcttcatc tttctgctcg ggcgggtatt acgcaccgat
     2701 accgcccagt caacgatcat cctcatcctg ctggggtacg gagtcggcat cgggttggcc
     2761 atcgctcagc tcacgaacat cgtgctcagc gaggtccccg cgaaccggtt gggcgcggga
     2821 tccggagcca acaacacgct gcgccaggtg ggagccgcgc tgggcaccgc ggtcattggc
     2881 gcggtcctga gcaccacgct ctcgctctcc gtgcagacgg gcctcaaagg cgtgtcggag
     2941 attcctgacg tggccaagac ggcgatcgtt gcatcgctgg accggggcgc gacgttctcc
     3001 gggggttccg tcggcgtcag cggagcaccc tcgggcgccg aaggaacgcc ggcggccgtc
     3061 gcggtggaga aggtcatcaa ggaatcgttc gtgaacgccg cccgagcggc cacggtcgcc
     3121 gcaagcctgt tcgtgctcct gggcgcggtc agctcactgc tgatcccaac gacggtgcgc
     3181 cgggccgagc cctcgcggct gggaagggtc gggtagaaat gtacaagaag atcatggtcc
     3241 ccctggatgg ctcgacagcg gccgaagcgg tcctggacca cgtcaggaag atcgctggag
     3301 aaggcaccga agtcgttctc gtgcgcatcg ccgccaagcc ggcctacgag tatacactcc
     3361 aggaacccca actctcggct tgcctggacg aggacgccgc gggagaggcg gggcaatacc
     3421 tgtcagaggt ggcggccagg ctcaagcggt ccgggctcga tgtctccacg tgcgtcctgc
     3481 cggagcaggg gccggccgcg cgggtcctgg aggattacgc ccacaaggca acggcggatc
     3541 tgatcgcgat ctcggcgcac ggcaagccgg gcctgatcgg gaggctgctg ggaagcgtgg
     3601 cggagcggat cgtgcaccac ggccgcatcc cggtgctcct ggtgcaccca tgagccggga
     3661 cggcaggcag gcggagaccg gacgctggcc cgacgggttc atcacgagag cttcgccgca
     3721 atcgcgccgg ccaggtcccg ggctgcgacg gtcagcgctt ctcccctgag cttgcccacg
     3781 ttgagggttt ctccagcgac catcctcgcg cctttggcct cgagggtgcc gcggatgatg
     3841 cccaaggcac gctcgggccg caggcctccg caccccagga cgaccgctac ttccttgcca
     3901 tgcaactccg cctcatccac gaaggtcatg agctggcacg cggggccgcc cgcccaaacc
     3961 ggggcgcaca ggatcaccga ttcaaaccga gacaggtccg tgtccatgga caggatggga
     4021 aacctcctgc ccatccttga tccgaacacc ttgccgaaaa acgagtgtcc gcgagcgctc
     4081 ctgatctctc gagcttgcag tccgagggca tcggacatgg tccgggcgat tcgcgcgttc
     4141 gtcccggtct cgctgtagaa aacgatgatg gtcatcgccc gtcaggcctc aagcggcttg
     4201 acggcgctcc gcaccacgga accgatgaca gccgccgcca ccacgcttcc acagacgacc
     4261 gccgcgacaa acactgcctg gctcgcggag ctggggtccg cggatcgctt catggcaatc
     4321 cccaccagcg cccagaggac cacgaggctg taggccacgt cccggcgagt cgcaagcacc
     4381 gccccggtga ggactgccgc cacgacgacc accgtcaccg cccagaactg gggctgcatc
     4441 ccaaagccca tccaacctgc cttcacgagg gcggccgtca cattggcgat ggtggcaacg
     4501 gttatccagc caaggtacac gctgaacggc acccggaaga gccatcgctc ggcctggcca
     4561 gcgggcgtgc gcccggtacc cagcctctgg tgaatggcaa caagagacgc gaggatgagc
     4621 agcatcgcca gtaccgacca tcctacgagc cggaagtgcc agaggaggag ccatccgata
     4681 ttgccggcgg atgacaggac aaacagccac ccaacctgcc tcgtcccctc cgggctcgtg
     4741 cggctggatg agaagagccc gcgcgcctgc ccgatggtga agagcaggag taggatgtag
     4801 atcactcccc agatcgagaa tgtcagcccg gcgggtacaa agagattggg gatctcgtct
     4861 gagagagcac cggtcagccg accgttgatt ccaacggtag ccgcaagcgt attcaccacc
     4921 aggactccaa gaaaacagac gatattgctg gtctggacaa tcttgattct tgattccatg
     4981 gcgtgagtct accatggcgg ccgagaagcc gcaaccgcga tactgatctt gaatatcgaa
     5041 tcgagttact ttcttcttta caaaccacga atagtgttat atactcctct catggttgcc
     5101 atgcagacca aaggggcctt cacggcgtcg ctcaccgagc acaaacggga gatcagcgcc
     5161 attcttgacg ccttctttgc ggctaggaga cgtgaccttt ccgaggtgaa ccagcttgcc
     5221 aacgaggcat gcgaccggct gcgccagttc gtgctggccg gcaagatgat tcgaggaggc
     5281 cttgtcacgg ttgggctctc gctctgccgt gacggcccga gtagccgcga acttcatatg
     5341 gctcaccagg tcggagccgc cattgagctt ttccagtccg gtctcctgat ccatgacgac
     5401 atcatggacc gcgacctcat gcgtcgcgga agggcaacgc tctttgagca gtacagcagg
     5461 gacgcgaggc agggcggtgt caacgactgg cgccacctgg gcgaagcgct ggggacgtgt
     5521 gtcggagatg ttgcgttctt cctcggatat gagctgctcg gagggctttc tcccagtcca
     5581 gcggccctcg ggaggatcat cgcgttgttc tcccgggagc ttgcggcggt cgggattgct
     5641 cagatgcagg atgtagcctg gggcgccgac ccttccgccc agcccggtac cgacgacatc
     5701 ctgcggctct accgattcaa gaccgggcgg tactccttct ccctccccct tgcggccggg
     5761 gccatcctgg ccggtgccgc cgacagctcc atcgaggccc tggaggcgct cggagaatgc
     5821 gtcggtgtga tcttccagat ccgcgacgac gagctgggcc tctgggggga cgagaagaag
     5881 actggcaaac cggtcggctc cgacatccgg gaaggcaaga aaacgctcct ctt
//
LOCUS       DASRZS010000512         8291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5285785, whole genome shotgun sequence.
ACCESSION   DASRZS010000512 DASRZS010000000
VERSION     DASRZS010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8291
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5285785"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VFH83_12510"
     CDS             <1..883
                     /locus_tag="VFH83_12510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6487240.1"
                     /translation="KKDFAEAYYMIGSILRDRGDLGGALESFKQAVQYAPANAGFMRE
                     LGSTMMARGDFPGAETTFTRALGLDSENPASNANMALAKIKLGKPSDALPYAQKAVAK
                     DPKSAPDNYILGLANERLGNVDEALRYYTSTVSYATVKDTDYLAALISLGGLYDGSGF
                     PDKALPYLQEAVQLQPASYEAHNNLGNVYLHKQQYADSVKELSRALAIQPDSKFTRFN
                     LGLAYSETGQTSEAKSDFQSVIQVDPSYFEAYIKLAEIYIKEGNPSDAKSLLDKLLQR
                     NPKADVRAEAQALIDKM"
     gene            complement(905..2674)
                     /gene="mutL"
                     /locus_tag="VFH83_12515"
     CDS             complement(905..2674)
                     /gene="mutL"
                     /locus_tag="VFH83_12515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET6487241.1"
                     /translation="MSDANPPSRRIRILREEVSRKIAAGEVIDRPLSIVRELVDNALD
                     AEARSIDVYLEAGGISRIRVVDDGAGMGPEDLDLCWQAHATSKIEHEDDLLRVTSLGF
                     RGEALSSIATCSRLSIQSSPRAAGERQGPAHRLEVRAGKRLSFEPGPGRNGTTVEVTE
                     LFHDFPARRKFLRSPSAESGLCRSVFVDRAVAHPDVGMRLFVDGDLKLTLPAGGLKDR
                     IALAYAQQLDGRLLREAGLEIEGARVRLVAGLPELRRRDRKLMQVFVNGRRIAEYALL
                     QAVEFGFSGTVPGGWHPVAFVLLDVDPSLVDFNIHPAKKEARFRNLPEVHRLVVAAVQ
                     RMLAPSVSSAASRGQAPSEAGPGMAGGANRPPAETHGPIPLIPREDRPAPAGDGLPIR
                     YLGQVFGVFLIFELPGRLVMLDQHAAHERLIYDRMGARAPALQEMLFPLAFDASEEEA
                     ARIEAARADLEALGIAVQPSSGGPTGGRRAGARSFEVTSLSSDFQALPAENLVELLRG
                     VGGVEPRPGSGADWKDHLRAGAACKLALKDGDPVDPVTARELCAGALRLAVPRCPHGR
                     PIWHQIAEDALRKLVDRPVSERG"
     gene            complement(2696..4942)
                     /locus_tag="VFH83_12520"
     CDS             complement(2696..4942)
                     /locus_tag="VFH83_12520"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HET6487242.1"
                     /translation="MTRKTTIVLLLAALLTLPGILFAQSKPATAKPAGVTVTITDWEN
                     TSGNAALLDDKGATQPEPSIGDKFPLGWTITTGKGDTAELQLDPTKTVVKIAQLTNFT
                     VKSLRSDTAGTDTFALTLGKVRTVVGRASGKDQYKIQTPSAVCGVRGSDVVIDTGDGT
                     QDKLFTLEGTGYMEKPDGTALDIPMGQFADALASVFQTVAIPADILSSLKSEMTFSPK
                     TDVAAANAAHQEDLQQSGGAGGSQQPGQSSGGTPSTPPPPKTNGFLDSIMKKLEDILG
                     MQIGSITIGGQTYSEAVISPTFALGKLKMALYLPIIYQGDMFNPSDYYHPAGNDEWDF
                     GGLLTGHPLADPWALTADLLNDLLLKIKYIEWGAQRDPFFFKLGNLDDITIGHGLIMN
                     DFSNDADFPAVRRVGLNIGADLGGFGFEAMVNDAAAPDVFGGRLYIRPIKGFKAALGL
                     STVVDIGAGQDFNGGPDAAGNPIFVNPGIDLDLPFVESDFFGLVAFADGAAMLPFFRT
                     APTAATGIPAGFALNAIYDASAQIPVKNWGVAAGLFGNLIIPGFTWRAEFRDFNGTFQ
                     PGFYSAGYERQRNALLQNLLAYLQNPTDPSFNQQTLGIYGEGGLTLPKLFSLNLNYFW
                     PWTQDPVTGQINTSADDQLSVTFTLDKGVIPVVNIYGSVSYTRTKFVSTIMNGGEQGL
                     TLGQALFDANTVVSASISYPVSDVLDVTLLYTTTAHRNAAGVLTYVNNPPLPDMDTSL
                     SIETQIHL"
     gene            complement(4958..6328)
                     /locus_tag="VFH83_12525"
     CDS             complement(4958..6328)
                     /locus_tag="VFH83_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487243.1"
                     /translation="MKEVSMPTGLRRFMLVLALLGLVTGPLFSQSNAGANAFGITMNL
                     GIGVQQFNEGVPPAPVTYQSISLAPDISFGKFGIGLAVALNYRFTGADNQFEIRAADW
                     VPNSFQDFLAIYLPKISYIRWGQKGDPLFLKFGSFDDGTLGDGFIVGDYANTLFLPAN
                     RHFGLAADLDAGMFQFPYVGIETFVGNLALLDVLGARLYVRPLVSTSIPILSNLEIGV
                     TGAWDTDPYAYTQGAFTTAQPVGVYGADIRVPVIAVQNVASMVAFTDFATINGKTMGG
                     MLGVGGKLFNFITYGTQLRLLGQDFIPNYFGPTYDLFKSTQYDLVQTGGFSPSSVGYY
                     ASIGTSFLNDKFIFNVSLDGPFGAIVPATASNATTADPHLRGVLSLADGVIPNITFDF
                     TYDKKAIDTWASLVSAQDANINAQFNFKSGAAVISFIYTITYSPSSTPNPWIVTSGLQ
                     SSIALF"
     gene            complement(6379..>8291)
                     /locus_tag="VFH83_12530"
     CDS             complement(6379..>8291)
                     /locus_tag="VFH83_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487244.1"
                     /translation="GAWHEVRVSQSDSTVKIDGTAIGASAASVSTDPGYGELSLFQFD
                     VTGPSGSPPPPSGLLYLDEVYLTDPSGSFGAALVGTFAASYPGPIVALGRIPILSNLD
                     LRQDLSMQTAGFAPLYGVPSGTDEIVSRTQAAADVLFARLAVDIRLLEAAGSLQAAGG
                     HKITLPAGGLPLSLSDSFDLSGSGGFSREDNLTLGLGWPVSAVVDSAASVDDLTGVLQ
                     QSWSAAATANLGLAASAALQLSQAVGGYPLPALSYFDRWVRELALLEPYQGGADLQRG
                     GKLDLKVSLPAAPLGFNGEVEADMAGTDYDAAGFTQQSDALLSLGLSLRLGEGTPNDT
                     TLSLGYQRSLALTTTPGPGPRFAAEGEELARLLALQTYLWEAIPLQEIFADTTAVVAP
                     DWLSVTKAIYVPSLDLSLQRGYGSRLLDLIVPDSVDLSLRQDVRREADLSSAIVTIAP
                     KVSTHAINLFGRLGAFPLVPWMDTDEYSLSVSGTAQAAPLTPLVVTTLSAEGYVAAKA
                     QGGAEVTLVETFKRDQTTSIDLSNDTQALLSWNYAPEGGIPIPLVPVELSSTVSLANQ
                     EKVEVTVAYNDTSSYHPFTLTLGHATSLVFPGHGTIKASLDVGADVENLLQEGVAWRL
                     AVRAALEAKLTF"
BASE COUNT         1513 a         2656 c         2745 g         1377 t
ORIGIN      
        1 caagaaggac ttcgcggaag cctactacat gatcgggtcc atcctccggg atcgcggaga
       61 cctgggcggc gcgctcgagt ccttcaagca ggcggtccag tacgctcccg cgaacgccgg
      121 gttcatgcgg gagctgggtt cgacgatgat ggcgcgggga gatttcccgg gcgccgaaac
      181 cacgttcacc cgcgcgctgg gcctggactc cgagaaccct gcctccaacg cgaacatggc
      241 cctggccaag atcaagctgg gaaagccgtc ggacgccctc ccgtacgcgc agaaggccgt
      301 cgccaaagac ccgaagtccg ctccggacaa ctacatcctg ggcctcgcga acgagcggct
      361 cgggaacgtg gatgaagcgt tgcgctacta caccagcacc gtgagctatg cgacggtcaa
      421 ggacaccgac tacctggccg cgctcatcag cctgggcggg ctgtacgacg ggagcgggtt
      481 cccggacaag gcccttccct acctccagga ggctgtccag ctccagcccg cctcctacga
      541 ggctcacaac aacctgggca acgtctacct gcacaagcag cagtacgccg acagcgtgaa
      601 ggagctatca cgcgcgctgg ccatccagcc ggactcgaag ttcacccggt tcaacctcgg
      661 gctggcctat tccgagaccg gccagacctc ggaggccaaa tcggacttcc agtcggtcat
      721 ccaggtggat ccctcgtact tcgaagccta catcaagctc gcggagatct atatcaaaga
      781 gggcaatccc tccgacgcaa agagcctgct cgacaagctg ctgcagagaa accccaaggc
      841 cgacgtgagg gctgaggccc aggcgctgat cgacaagatg taggcgctca cggaaagcgg
      901 ccgctcagcc gcgctctgaa accggtcggt ccaccagctt gcgcagggcg tcttcggcga
      961 tctggtgcca gatgggccgg ccgtgggggc agcgggggac ggcaagccgc agcgcgccgg
     1021 cgcagagctc gcgggccgtg accgggtcca ccggatcccc atccttcaga gccagcttgc
     1081 aggccgcgcc ggcgcgcagg tggtccttcc aatcagcccc actcccgggg cggggctcta
     1141 cgcctccgac tccccggagc aactcaacca ggttctcggc aggcagcgct tggaagtcag
     1201 aagacagaga agtcacctcg aacgaacggg cgccggcgcg cctgccgccg gtgggcccgc
     1261 cgctgctcgg ctgcacggca atccccagag cctccaggtc ggcgcgggca gcttcgatcc
     1321 gcgcggcctc ttcctccgat gcatcgaagg cgagcgggaa aagcatctcc tgcagcgccg
     1381 gcgcccgtgc tcccatgcgg tcgtagatca gccgctcatg agcggcgtgc tggtccagca
     1441 tcaccaatcg gccgggaagc tcgaagatca gaaacacccc gaacacctgc cccaggtagc
     1501 ggatcggcaa cccgtctccg gcgggagccg gcctgtcctc ccttgggata agagggatcg
     1561 gaccatgggt ctcggcggga ggccggttgg cgccgcctgc cataccggga ccggcctcag
     1621 aaggcgcctg gcctcgagac gccgccgagc ttacggacgg cgccagcatc cgctgcaccg
     1681 ccgccacgac caggcgatgc acctcgggca ggttgcgaaa ccgcgcttcc ttcttggcgg
     1741 gatggatatt gaagtccacg aggctgggat ccacgtccag caggacgaaa gccacgggat
     1801 gccatccccc gggcaccgtg ccggaaaagc cgaactccac cgcctggagc agggcgtact
     1861 ccgcgatccg tcggccgttg acgaagacct gcatcagctt ccgatcccga cggcgaagct
     1921 ccggcaggcc cgcaaccagt ctcacccggg ctccctcgat ctcaaggccg gcttccctca
     1981 ggagtcgtcc atcgagctgt tgagcatacg ccagggcaat ccgatctttc agaccgccgg
     2041 cgggaagcgt cagcttgaga tcgccatcga cgaacaggcg catcccgacg tcagggtgcg
     2101 cgaccgcgcg atccacgaat accgaccggc agagcccgga ttccgcggac gggctcctca
     2161 gaaacttgcg tcgtgccgga aagtcgtgga acagctcggt gacctcaacc gtcgttccgt
     2221 tgcggccggg gccgggctcg aaggagagcc gtttccccgc gcggacttcc agccggtggg
     2281 cagggccctg ccgctcgcca gccgccctcg gggagctctg gatcgaaagc cggctgcacg
     2341 tggcgatgct ggagagggcc tctccacgga accccaggct ggtgacccgc agcaggtcgt
     2401 cttcgtgctc gatctttgat gtggcatgcg cctgccagca gaggtccagg tcttcgggcc
     2461 ccatccccgc gccatcgtcg accacgcgga ttcgggagat ccctcccgct tccaggtaga
     2521 cgtcgatgga tcgagcctcc gcgtccagcg cgttgtcgac gagctccctt acaatggaaa
     2581 gcggccggtc gatgacctcg ccggccgcta tctttcggga aacctcctcc cggaggatcc
     2641 ggatccgccg ggaaggaggg tttgcatcgg acatgcgccc cttcgggccc tcgccctaca
     2701 ggtggatctg cgtctcgatg gaaagggacg tgtccatgtc cggcagcggc gggttgttga
     2761 cgtacgtgag cacccccgcc gcgttgcggt gcgccgtggt cgtgtacagc aacgtcacgt
     2821 ccagcacgtc ggacaccggg tagctgatgc tcgcgctgac gaccgtgttg gcgtcaaaca
     2881 gcgcctggcc gagcgtcaat ccctgctccc cgccgttcat gatcgtcgag acgaacttgg
     2941 tgcgggtata cgagaccgac ccgtagatgt tcaccacggg aatcactccc ttgtcgagcg
     3001 tgaacgtgac cgacagctgg tcatccgcgg aggtgttgat ctgccccgtc accgggtcct
     3061 gggtccacgg ccagaagtag ttcaggttga ggctgaacag cttgggcagc gtcaagccgc
     3121 cctccccgta gatgccgagg gtctgctggt tgaacgacgg gtccgtggga ttctggagat
     3181 aggcgagcag gttctgcaac agcgcgttgc gctgcctctc gtagcccgcg gaatagaagc
     3241 ccggctggaa cgtgccgttg aagtcgcgga actccgctct ccaggtgaat ccggggatga
     3301 tcaggttgcc gaagagccct gccgccacgc cccagttctt gacggggatc tgcgcgctgg
     3361 cgtcgtagat cgcgttcagg gcgaagccag ccgggatccc ggttgccgcg gtcggagcgg
     3421 ttcggaagaa cggcagcatg gccgctccat ccgcaaaggc gaccaggccg aagaagtcgc
     3481 tctccacgaa cggcaggtcc aggtcgatgc ccgggttgac gaagatggga tttcccgccg
     3541 cgtccggacc cccgttgaag tcctgcccgg ctccgatgtc cacgacggtg gacaacccca
     3601 gtgccgcttt gaaccccttg atgggccgga tgtacaggcg gccgccgaag acgtcgggcg
     3661 ccgcggcgtc gttgaccatg gcctcgaacc cgaaaccgcc caggtccgcg ccgatattca
     3721 gccccacgcg gcggaccgcg gggaagtccg cgtcgttgga gaagtcgttc atgatcaggc
     3781 cgtgcccgat cgtgatgtcg tccaggttcc ccagcttgaa gaagaaggga tcccgctgcg
     3841 cgccccactc gatgtacttg atcttgagga gaaggtcatt cagcagatcc gccgtgagcg
     3901 cccaggggtc ggccagaggg tggccggtca gaagaccgcc gaaatcccac tcgtcgttgc
     3961 cggccggatg gtagtagtcg gacgggttga acatgtcccc ctggtagatg atgggcagat
     4021 acagagccat cttgagcttg ccgagggcga acgtcggaga gatgacggcc tcggagtagg
     4081 tctgcccacc gatggtgatg gagccgatct gcatcccgag gatgtcctcg agcttcttca
     4141 tgatgctgtc caggaagccg ttcgtcttcg gaggcggagg cgtcgacggc gtcccgccgg
     4201 aagactggcc cggctgctgc gagccgccgg ccccgccgga ctgctggagg tcttcctggt
     4261 gcgccgcgtt ggcggccgcc acgtccgtct tgggagagaa cgtcatctcg ctcttgaggc
     4321 tggaaaggat gtcggcggga atggcgaccg tctggaaaac ggatgccagg gcgtcggcga
     4381 actggcccat ggggatgtcc agcgcggtcc cgtcgggctt ctccatgtag ccggtgccct
     4441 cgagcgtgaa cagcttgtcc tgggtcccgt cgccggtatc gatgacgacg tccgacccgc
     4501 ggactccgca gacggcggac ggggtctgga tcttgtactg gtcctttccc gaggcccgtc
     4561 cgacgaccgt tcggaccttg ccgagggtca aggcgaaggt gtcggtcccc gccgtgtccg
     4621 agcgaaggct tttgacggtg aagttcgtga gctgcgcgat cttgacgacc gtcttggtcg
     4681 gatcgagctg gagctcggcc gtgtcaccct tgccggtcgt gatggtccac cccaacggga
     4741 acttgtcccc gatgctcggc tccggctggg tcgccccctt gtcatccaga agagccgcgt
     4801 taccgctcgt gttctcccag tcggtgatgg tcaccgtaac ccccgcgggc ttcgcggtgg
     4861 ccggcttgga ctgcgcaaag aggattcccg gcagcgtcag gagcgccgcc aggagcaata
     4921 cgatcgtggt ctttctcgtc atgggagcct ccgtttccta gaagagggcg atggagctct
     4981 ggagcccgga tgtcacgatc cacgggttcg gggtgctgct cggtgaatac gtgatggtgt
     5041 agatgaagga gatcaccgcg gctccgctct tgaagttgaa ctgggcattg atgttcgcat
     5101 cctgggcgct gaccaggctg gcccacgtgt cgatggcctt cttgtcatac gtgaagtcga
     5161 aggtgatgtt ggggatcact ccgtccgcca gcgacagcac gccgcgaagg tgaggatcgg
     5221 ccgttgtcgc gttggacgcg gtggccggca cgatcgcgcc gaagggcccg tccaggctga
     5281 cgttgaagat gaacttgtcg ttcaggaacg acgtcccgat gctggcatag tagcccacgc
     5341 tggacgggga gaagccgccc gtctggacaa ggtcgtactg ggtgctcttg aacaggtcat
     5401 aggtcggccc gaagtagttg gggatgaaat cctggcccag cagacggagc tgcgtcccgt
     5461 aggtgatgaa gttgaagagt ttcccaccga cgccgagcat tccgcccatg gtcttgccgt
     5521 tgatggtcgc gaagtccgtg aaggcaacca tcgaggcaac gttctgcacg gcgatcaccg
     5581 ggacccggat atccgcccca tagactccga ccggctgcgc cgtcgtgaag gcgccctgcg
     5641 tgtacgcgta cggatcggta tcccaggcgc cggtcacgcc gatctccagg ttgctgagga
     5701 tcggtatgct ggtggagacg aggggccgaa cgtagagacg ggctcccagg acgtccagca
     5761 acgccaggtt gcccacgaac gtctcgatcc ccacgtaggg gaactggaac atgccggcgt
     5821 ccaggtcggc ggccaggccg aaatgccgat tcgccgggag gaacaacgtg ttggcataat
     5881 ccccgacgat aaacccgtca ccgagggtcc cgtcgtcgaa cgagccgaac ttgaggaaca
     5941 gggggtcacc cttctgcccc catcggatgt agctgatctt ggggaggtag atggccagga
     6001 aatcctggaa gctgttgggc acccagtcgg cggcccggat ctcgaactgg ttgtccgccc
     6061 cagtgaaccg atagttgaga gcgacggcaa gcccgatccc gaactttccg aaagagatgt
     6121 ccggagcgag gctgatggac tggtaggtaa cgggagccgg aggcaccccc tcgttgaact
     6181 gctgaacgcc aatgccgaga ttcatcgtta tgccgaaagc gttcgcgccg gcgtttgact
     6241 gcgagaagag cggtcccgtc actaagccga gcagtgccag gaccagcatg aagcgcctga
     6301 gacctgtagg catggagacc tccttcatgt cggaaccaat tataacccgt caattcctgg
     6361 aatgctaact cccgtacctt agaaagtcaa cttggcttct aacgccgcgc ggacggcgag
     6421 gcgccacgcg acgccctcct ggagcaggtt ctccacgtcg gccccgacgt ccaggctggc
     6481 tttgatcgtc ccgtggcccg gaaacaccag ggaggtggca tggccgaggg tcagcgtgaa
     6541 cggatggtac gaagaggtgt cgttgtaagc taccgtgacc tccactttct cctggttggc
     6601 gaggctcacg gtcgaggaca gctcgacggg aaccagggga atagggatgc cgccttccgg
     6661 cgcgtagttc cagctcagga gcgcctgggt gtcgttggag aggtcgatcg acgtggtctg
     6721 atcgcgcttg aaggtttcca cgagcgtgac ctccgctccg ccttgcgctt tcgccgcgac
     6781 atacccctcc gcggagagcg tcgtaacgac gagcggagtg agaggagccg cctgcgccgt
     6841 tccgctgacg ctcaggctgt actcatcggt atccatccaa gggacgaggg gaaacgctcc
     6901 cagcctgcca aagaggttga tggcgtgcgt ggaaaccttt ggcgcaatgg tcacgatggc
     6961 cgacgagagg tcggcctcgc gtcgaacgtc ctgccggagg gaaagatcca cggaatccgg
     7021 gacgatcagg tccagcagcc gggatccgta gccccgctgc agtgagagat cgagggacgg
     7081 aacgtagatg gccttggtga cggagagcca atccggagcg accaccgccg tggtgtccgc
     7141 gaagatctcc tgcaagggaa tggcctccca gagatacgtc tgcagggcca gaagccgggc
     7201 cagctcctcc ccttccgcgg cgaaccgcgg tcccggacca ggagtcgtgg tgagagcgag
     7261 cgaacgctgg taccccaggc tcagggtggt gtcgttcggg gtcccttctc ccagccggag
     7321 ggagagcccc agggacagaa gcgcgtcact ctgctgcgta aacccagccg catcgtagtc
     7381 ggtgccggcc atgtccgctt ccacctctcc attgaacccc agcggagccg ccggaaggga
     7441 caccttgagg tccagcttgc ccccgcgctg cagatccgct cctccctggt aaggctcgag
     7501 gagcgcgagc tcccgaaccc accggtcgaa gtaggagagc gccggcagcg ggtagccgcc
     7561 gacggcctgg gagagctgga gagcggcgct cgcggccagg ccgaggttcg cggtggcggc
     7621 cgcgctccag gactgctgca ggacgcccgt caggtcatcg accgaggcgg cgctgtccac
     7681 gacggcgctc accggccagc caagacccaa ggtcagattg tcctcccgag agaatccgcc
     7741 cgaaccggag aggtcgaatg agtcggacag cgacagagga agcccacccg cgggcagggt
     7801 gatcttgtgg cctcccgccg cctgcaagga tcccgcggct tccagcagcc ggatgtcgac
     7861 ggccagccgc gcgaacagga cgtccgctgc tgcctgggtc ctgctgacga tctcgtccgt
     7921 tcccgacggc actccataca gcggcgcgaa gccggccgtc tgcatgctga gatcctggcg
     7981 caggtcgagg ttggagagga tggggatcct gcccaacgcc acgatcggac ccggatacga
     8041 cgcggcgaac gtgccgacga gcgctgctcc gaaggatccg ctgggatccg taaggtagac
     8101 ctcgtcgagg tagaggagcc cgctgggagg cggcgggctg ccggacggac ccgtcacgtc
     8161 gaactggaac agagaaagct caccataacc cggatcggtg ctcaccgagg cggcgctcgc
     8221 cccaatcgcc gttccgtcga tcttgacggt ggaatccgac tgggagacgc gcacctcgtg
     8281 ccaggcgcca t
//
LOCUS       DASRZS010000513         5753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2195095, whole genome shotgun sequence.
ACCESSION   DASRZS010000513 DASRZS010000000
VERSION     DASRZS010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195095"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VFH83_12535"
     CDS             complement(<1..822)
                     /locus_tag="VFH83_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487245.1"
                     /translation="MADLTQESWLQRVRERGLKWALAPGALVILYLFFGAFGINYFSY
                     PTLVNIIDAAYYFGFIAMGVTFVIISGGIDLSIGTVMMCAAITGGTAIKYGLPMVPGL
                     LLIIAVGLAFGAFNGLLVSRLKMPPFIVTLGTMMISMGVGSIVSNVSSATFPIRGQPG
                     GWFKSIFLFIGDNGATIPTGALLVAIVAVICHLMLSNTKLGRYTYAIGSNLEAARLSG
                     VDVKKWQLLVYIIAGGTAGIGGISYAAIYTTVLPQQGQGFELNAIAAAVIGGTSLS"
     gene            complement(826..2340)
                     /locus_tag="VFH83_12540"
     CDS             complement(826..2340)
                     /locus_tag="VFH83_12540"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6487246.1"
                     /translation="MSRQVLSMRKIDKRFSGVHALRQVDFDLDEGEIHSLVGENGAGK
                     STLMKVLVGINPKDSGEIHYLGHLFNPRDPKHALEMGIGIIHQELNMMEHLTVAENIF
                     IGRESTRGMLLDKRAQNERAAELFRRLKMNIDPMEPLSRLTVGKQQMVEIAKAVSHRL
                     RILILDEPTAALTDAEIDELFTIMRDLASRGVGMIHISHRLDEIHRIADRVTVLRDGE
                     RMGTKKTADVSRQEIINMMVGRVISFQPKTASEVPADAPVVLKVSHLNAGRLVRDVSF
                     ELRRGEILGVAGLMGSGRTETARAIFGADEVQSGSIEIRGRRALIRSPEDAVAHGIGY
                     LSEDRKRYGLAVNLSVRDNLALATYDRFQKGLFIHAAAVRQVTQDYIQRLSIRTPSLE
                     QLLRNLSGGNQQKVVIAKWLIRACDVLIFDEPTRGIDVGAKSEIYALMTELTREGKSI
                     IMISSELPEILRMSDRIIVMSEGRITGELPIEKASQEACMELATIGHQAVLSRS"
     gene            complement(2492..2794)
                     /locus_tag="VFH83_12545"
     CDS             complement(2492..2794)
                     /locus_tag="VFH83_12545"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HET6487247.1"
                     /translation="MLFQTGTYQVKRSAIPQVKKAIEEFVAYVKANEPGTLMYLAWQQ
                     KTDPTRFVHLFIFQDEAAHQRHGESAAVKRFESVYGPELSGGEVVFTDYEMIAGKR"
     gene            complement(2986..4152)
                     /locus_tag="VFH83_12550"
     CDS             complement(2986..4152)
                     /locus_tag="VFH83_12550"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF044362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6487248.1"
                     /translation="MPPTVEAARIRELRERLLARLLTIIAAAGTLAYGPSAYLSIREG
                     LWVILAVDTLACVSAVCLALIRALPFRLKAGYLVGLTYALGVLLLAYTGEFGAGHLFL
                     FAFVFLAALFGEVWVMIAANGLSVLTYAAFYFAVASNRLEWPQGSGPVLVVSVNAILI
                     SLVLSVSANYLLRGYASAASEEERLRRTVEMMLKELEHRVKNNLQVISSLISLRSRST
                     SDPSRALEEIHESVSALVVVHQLLYRRSGVNRLSVKVLLGSLAERFRNLFRDVSILFE
                     WNGPDLEIDGDRAVSLGLMVNEIVMNASKHAFAGGTEASIAIRVDHQEPTRSVALSVG
                     DNGRGMDPGAAEGVGIQMIRALARQLGAQMDVRHAPGVQYRFDIPVDPTSRETR"
     gene            4293..5285
                     /gene="mgrA"
                     /locus_tag="VFH83_12555"
     CDS             4293..5285
                     /gene="mgrA"
                     /locus_tag="VFH83_12555"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006038769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HET6487249.1"
                     /translation="MYLAHEQRYSSMSYRRCGSSGLRLPAISLGLWHNFGGIDRFDSM
                     RSLLRFAFDHGVTHFDLANNYGPPPGAAEENFGRILHADLGAYRDEMVISTKAGFTMW
                     PGPYGDWGSRKYLVASLDQSLKRMGLDYVDIFYHHRPDPDTPLEETLEALDQVVRQGK
                     ALYVGISNYSAEQTERAVAVLGSLGRRLLIHQANYSMLNRWIENGLVSLLKREGSGII
                     AFCPLAQGLLTDRYLEGVPADSRARKPVAFLREKDLEPAKLAKVRKLREVAHARGQSV
                     AQMALAWVLRDPGVTSALIGASRVEQIEENIGALQRLDFTAEELESVERILADK"
     gene            complement(5222..>5753)
                     /locus_tag="VFH83_12560"
     CDS             complement(5222..>5753)
                     /locus_tag="VFH83_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HET6487250.1"
                     /translation="LVHIILEQQVSLASARAAFDRLKEATRPITPKGFLMLGDEELKR
                     IGFSRQKAAYCRGLASAIQSRELRLKALGEAEDDAARSALLAVRGIGPWTADIYLLMV
                     LLRPDVWPTGDMALAVAYQERYRLRERPSQERLGRIAERWRPWRAVAARLLWHDYLSA
                     RIRSTDSSSSAVKSSR"
BASE COUNT         1011 a         1893 c         1869 g          980 t
ORIGIN      
        1 cgacagcgac gtgccgccga tgaccgccgc ggcaatggcg ttcagctcga acccctgccc
       61 ctgctgcggc agcaccgtgg tgtagatggc cgcgtaggaa atcccgccaa tgccggccgt
      121 tccgcccgca atgatgtaga cgagcagctg ccatttcttg acatcgacgc cggagagacg
      181 agctgcttcc aggttgctgc cgatggcgta cgtgtagcga ccgagcttgg tgttggacag
      241 catcaggtgg cagatcacgg ccacgatcgc caccaggagc gccccggtgg ggatcgtggc
      301 cccgttgtcc ccgatgaaga ggaagatgct cttgaaccag ccgccgggct gtccacggat
      361 ggggaacgtg gcgctggaga cgttggacac gatggacccc acgcccatgg agatcatcat
      421 ggtgccgagc gtcacgatga acgggggcat cttcagccgc gagaccagga ggccgttgaa
      481 agccccgaag gccagcccca ccgcgatgat gagcaggagc ccgggtacca tgggtagccc
      541 gtacttgatc gccgtgcccc cggtgatcgc cgcgcacatc atgacggtgc cgatggacag
      601 gtcgataccg ccgctgatga tgacgaacgt cacccccatg gcgatgaagc cgaagtaata
      661 cgcggcgtcg atgatgttca ccagcgtggg ataggagaag tagttgatcc cgaacgcgcc
      721 aaagaagagg tacaggatca ccagcgcgcc cggtgcgagc gcccatttca ggccgcgttc
      781 gcgcacgcgc tgcagccaac tctcctgggt caagtccgcc atgcgtcaac tcctgctgag
      841 aacggcctgg tgcccgatgg ttgccaactc catgcaggct tcctggcttg ccttctcgat
      901 gggaagctcc ccggtgatcc ggccttctga catgacaatg atgcgatcac tcatgcgcag
      961 gatctcgggc agctccgatg agatcatgat gatcgacttt ccctcccgcg tgagctccgt
     1021 catcagggca tagatctcgc tcttggcccc gacatcgatg ccgcgcgtgg gctcgtcgaa
     1081 aatgaggacg tcacaggcac ggatcagcca cttggcaatg actaccttct gctgattgcc
     1141 accggagagg ttgcgcagga gctgctccag ggacggcgtc ctgatactca gcctctggat
     1201 gtagtcctgg gtcacctggc ggaccgccgc cgcgtggatg aagaggccct tctggaagcg
     1261 gtcataggtc gcgagggcga ggttgtcgcg cacgctcagg ttgaccgcca gcccgtagcg
     1321 cttgcggtcc tcggaaaggt atccgatccc gtgggccacc gcgtcctcgg gagaccggat
     1381 gagagcgcgc cgtccgcgga tctcgatgct gccgctctgc acctcgtcgg cgccgaagat
     1441 ggcccgcgcc gtttcggtcc gacccgaacc catgaggccg gccacgccga ggatttcccc
     1501 ccggcgcagc tcgaagctca cgtcgcgcac caggcgcccc gcgttcaggt ggctcacctt
     1561 caggacgacc ggggcgtctg ccggcacctc ggacgccgtc ttgggctgga acgagatcac
     1621 gcgtcccacc atcatgttga tgatttcctg ccggctcacg tccgcggtct tcttcgttcc
     1681 catccgctca ccgtcacgca gcacggtgac gcggtccgcg atgcggtgga tctcgtccag
     1741 gcggtgcgag atgtggatca tccccacgcc gcgggacgcg agatcccgca tgatcgtgaa
     1801 cagctcgtcg atctcggcgt cggtgagcgc cgcggtgggc tcgtccagga tcaggatacg
     1861 aagccggtgg gacaccgcct tggcaatctc caccatctgc tgcttgccca ccgtcaggcg
     1921 ggacagcggt tccatgggat caatgttcat cttcagccgg cggaacagct ccgcggcgcg
     1981 ctcgttctgg gcgcgcttgt ccaggagcat cccccgcgtg gactctcggc cgatgaagat
     2041 gttctccgcc accgtgaggt gctccatcat gttcagttcc tgatggatga tcccgatccc
     2101 catctccagg gcgtgcttgg gatcccgggg attgaacagg tgtcccaggt agtggatctc
     2161 gccggagtcc ttgggattga tgccgaccag gaccttcatg agggtcgact tgccggcacc
     2221 gttctcaccg accagggagt ggatctctcc ctcgtcgagg tcgaaatcca cctgccgcag
     2281 ggcgtggact cccgagaaac gcttgtcgat ttttcgcatc gataggacct ggcggctcac
     2341 cggtatagct cctcgtgcgt aatccaaaga tcgcaccgtt tcccgcgcag gtaaagaagg
     2401 cttcacggcg gcggggatgg cgcgcgcggc gatggagcgt gagccggcca ggtcctacgg
     2461 cggcctgcgg aaggctcgcc gccgggaggg ttcagcgctt gccggcgatc atctcgtagt
     2521 ccgtgaagac gacctcgccg ccggacagct cgggaccgta gaccgactcg aagcgcttga
     2581 ccgcggccga ctccccgtgg cgctggtgcg ccgcctcgtc ctgaaagatg aagaggtgaa
     2641 cgaacctcgt cggatcggtt ttctgctgcc aggccagata catgagcgtc cccggctcgt
     2701 tcgcctttac gtacgcgacg aactcctcaa tggccttctt gacctggggg atggcggacc
     2761 gtttcacctg gtacgtcccc gtctggaaaa gcatcctgca cctcctggtt ctgccgagcc
     2821 tcctccgagg ggcgcaggtt cgcacccggg gtggcgcggc aggctcgaag atatgcaagc
     2881 aaatgcttgc aagtcaagag gaatccgcag ggaggcaacc ctcacccagg cggggcccgc
     2941 cctgatgggt tccgcgttac cggggcccac gcgaaggcac ccgcatcacc gggtctcgcg
     3001 cgaggtcgga tccacgggaa tgtcgaatcg gtactggacg cccggtgcgt ggcggacatc
     3061 catctgcgct ccgagctggc gggcaagggc gcggatcatc tggatcccca ccccctccgc
     3121 cgcgcccgga tccatccctc tgccgttgtc tccaacgctg agcgccaccg aacgggtcgg
     3181 ctcctgatgg tccacgcgga tggcgatcga tgcctcagtg ccgcccgcaa aggcatgctt
     3241 ggaggcgttc atcacgatct cgttgaccat gaggcccagg ctcacggccc ggtcgccatc
     3301 gatctccaga tcgggtccgt tccattcgaa gaggatggac acgtcgcgga acaggtttcg
     3361 aaaccgctcc gccagggaac ccaggagcac cttcacggag aggcggttga cgccgctcct
     3421 gcggtagagg agttggtgga cgaccacaag cgccgaaacg ctttcgtgga tctcctcaag
     3481 ggcccggctc ggatcggagg tggaacggct ccgaagcgag atcaggctgg agatgacctg
     3541 gaggttgttc ttcacccggt gctccagctc cttcagcatc atctccacgg tgcgtcggag
     3601 ccgctcctcc tccgaggcgg ccgacgcata accccgaagc aggtagttgg cggacacgga
     3661 gaggaccaga ctgatcaaga tggcgttcac cgataccacc aggacgggac ccgatccctg
     3721 cggccactcc aggcgattgc tcgcaacggc gaagtagaaa gccgcgtagg tcaggacgga
     3781 cagcccgttc gccgcgatca tgacccacac ctctccgaag agggcggcca ggaagacgaa
     3841 cgcgaagagg aagagatggc cggcgccgaa ctcgccggtg taggccagga gaagcacgcc
     3901 cagcgcatag gtcagcccca ccaggtagcc ggccttgagc cgaaagggca gggcgcgaat
     3961 cagggccaga cacacggccg agacgcacgc cagggtatcc acggcgagaa tcacccagag
     4021 cccctcccgg atcgagaggt aggcgctggg tccgtaagcc agcgtgccgg cggctgcaat
     4081 gatcgtcagc agacgcgcca ggagccgttc gcgcagctct cgtatgcgcg cggcctctac
     4141 ggtcggcggc atggtccgcg attctcctcc ggggatggtt tcacccgggc actccgggcg
     4201 ctactctaag tgtagcgttc ctctccggcg aaaacccgcg gagtgggggc gcccacccga
     4261 cttcgatcgg gcccacaagg aggattgccg tcatgtacct tgcccatgag caacgttact
     4321 cgtccatgag ctaccgccgt tgcggcagct cgggtctgcg ccttcccgcc atctcccttg
     4381 gcctctggca caacttcgga ggcatcgacc ggttcgattc catgcggtcg ctcctgcggt
     4441 tcgccttcga ccacggcgtg acccatttcg acctggccaa caactacggc cctccccccg
     4501 gcgcggcgga ggaaaacttc gggcggatcc ttcacgccga cctcggcgcg tatcgcgatg
     4561 agatggtgat ctccaccaag gccgggttca cgatgtggcc cgggccctac ggggattggg
     4621 gctctcgcaa gtacctggtg gccagcctcg accagagcct gaagcggatg gggttggact
     4681 acgtcgacat cttctaccac caccggccgg accccgacac gcccctggag gagaccctgg
     4741 aggccctgga tcaggtcgtc cgccagggga aggccctgta cgtggggatc tccaactact
     4801 ccgccgagca gacggagcgg gccgtcgcgg tgctgggttc cctgggccgc cgcctcctca
     4861 tccaccaggc caactactcg atgctcaacc gatggatcga gaacggattg gtcagcctgc
     4921 tcaaacgcga gggcagcggg atcatcgcgt tctgcccgct ggcgcagggg cttctgacgg
     4981 atcggtacct ggagggggta cccgccgatt cacgcgcccg aaagccggtg gcatttctgc
     5041 gggagaagga tctggagccc gcgaagctcg caaaggtccg caagctgcgg gaggtggctc
     5101 acgcccgcgg gcagtcggtt gcgcagatgg ctctggcctg ggtgcttcgg gaccccgggg
     5161 tgacctcggc gctgatcggc gccagccggg tcgagcagat cgaggagaac atcggcgccc
     5221 ttcagcggct ggacttcacc gcggaggagc tcgagtccgt ggagcggatc ctggccgaca
     5281 agtaatcgtg ccagagcagg cgcgccgcaa cggcgcgcca tgggcgccag cgctcggcga
     5341 tccggcccag ccgctcctgg ctcggtcgct cccgcagccg gtagcgctcc tggtaggcta
     5401 cggcgagcgc catgtcgccc gttggccaca cgtcgggccg caggagcacc atgagcaggt
     5461 agatgtccgc ggtccacgga ccgatccccc ggaccgccag cagcgccgat cgggccgcgt
     5521 cgtcctccgc ttccccgagg gccttcaggc ggagctctct ggactggatt gcggacgcga
     5581 gcccccggca atacgcggct ttctgtcgac tgaagccgat gcgcttcagc tcctcatcgc
     5641 cgagcatcag gaagcccttc ggcgtgatcg gacgggtggc ctccttcagg cggtcgaacg
     5701 cggcgcgagc ggatgccagg gatacctgct gctccaggat gatgtgcacc agg
//
LOCUS       DASRZS010000514         4064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1824129, whole genome shotgun sequence.
ACCESSION   DASRZS010000514 DASRZS010000000
VERSION     DASRZS010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824129"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFH83_12565"
     CDS             <1..305
                     /locus_tag="VFH83_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6487251.1"
                     /translation="AVIYAGRVLESAPRDPLFHRPLHPYTRLLMQSVPSAARRGARLP
                     AIPGRVPDGRALPPGCAFHPRCPIAEPLCSREDPPSVERAPSHAAACHLVEKPWPL"
     gene            293..1258
                     /locus_tag="VFH83_12570"
     CDS             293..1258
                     /locus_tag="VFH83_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487252.1"
                     /translation="MAPVGAAPLVQTRALVKRHYARGLLARGGSGTLAVDRVSLEIER
                     ARVFGLVGESGCGKSTLARALVWLDPPTSGDVFYDGVSLGTLSRRGLRALRTRMQIVF
                     QDPNGALDPRMRVRQSMEEGLVTIGMPAAERERRVDELLDLVGVSPESASRYPHEFSG
                     GQKQRIVVARALTLAPEFLVLDEPVSNLDVSVQAQIINLLMDLKERFSLTYLFITHDL
                     HLVAYLSDRIGVMLRGRLVENASAEELLSHPLHPYTVRLFASVPGVDASHPPQPGRLA
                     DAAESVRAGCPFRPSCDRALSICAIEDPELRDVGAGHLAACHALV"
     gene            1297..2793
                     /locus_tag="VFH83_12575"
     CDS             1297..2793
                     /locus_tag="VFH83_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6487253.1"
                     /translation="MNRNLCAALLACLLLLPGGALSARDKALNFALSGNPDTLDPQKT
                     SGTLTFQTLKSIYDTLVEPDQTGAIVPALAESWTVSADNLTWTFKLRPGVFFHNGDRL
                     DSADVKATLDRMRDKATASPKVVEFTTIASIDTPDDATVVIRLSKPSAPFLATLASGW
                     AAILPKSLIDSGNDFATRPVGTGPFMLKEFVRDSRIVLVKNPRYWMPGLPRLDQVIMN
                     IIPERSVQVQGLLTGAIDAVEFVNTDDIQLLASSSDVKIDKSLTADIMVMSMNCSNPI
                     LSDLRVRQAVTYAIDKQKVLDIAYGGGKVIGTFMDAANAYYKDFTSLYPYNPDKARSL
                     LKEAGVPADAVFTMVLPSNYDLHVKAGQLYQEMLSNVGLKVNIKMVDWPTWISDVYTN
                     ARYDFTVIGHTGKLDPDGTLSGYGGKSRYVRWDNPAALDLITRAAGVTGFDARKKLYD
                     QALEIMAREVPFVYLGSAYRYRAYRSNVSEYRMTPKLDTYDFRWTDLK"
     gene            2790..3614
                     /locus_tag="VFH83_12580"
     CDS             2790..3614
                     /locus_tag="VFH83_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487254.1"
                     /translation="MKRSGRRGGSLAYRLALAYLLILGGAAVAAPWIAPHSPTAQNLS
                     QRFAPPSRDHLLGTDDFGRDVLSRIIHGSRAALLVGVVSVAIAVVLGMTAGLLAALAP
                     GFVDNAVMLVMDSLLSFPTVLLAITVVSFLGYGLVPVMIAIGVIYSPTFARLVRAEAL
                     VIKAEEYVDASRSLGSGPLKVAMRHIVPNLSGKVIVQASVTFALAVVVEASLSYLGLG
                     TQPPAASWGLMLKDARNFLVQAPWMALYPGLVLALTVLSFNVIGDRLAERLNPKLG"
     gene            complement(3634..>4064)
                     /locus_tag="VFH83_12585"
     CDS             complement(3634..>4064)
                     /locus_tag="VFH83_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6487255.1"
                     /translation="GDEDVIDYIKHMARPLIFSASMPPATIASVRACLDIIEQEPERV
                     NRLQSIGCKMVSGFKALGFDVGTAETPIVPLLIGDMEKTLLFWRALFDGGVFTNPVLS
                     PAVPPDSTLIRTSYMAIHTDQECDHILEVAGEAGKKLGII"
BASE COUNT          642 a         1381 c         1324 g          717 t
ORIGIN      
        1 tggcggtcat ctacgccggc cgcgtgttgg agtccgcgcc gcgcgatccg ctcttccacc
       61 ggcccctgca cccgtacacg cggctcctga tgcagtcggt gccatccgcg gcgcggcgcg
      121 gcgcgcggct gccggccata cccggccgcg tcccggacgg gcgcgcgctg ccgcccggat
      181 gcgccttcca tccgcggtgc cccatcgccg agccgttgtg ctcgcgggag gacccgccca
      241 gcgtcgagcg cgcgccctcc cacgccgcgg cctgccacct ggtggagaag ccgtggcccc
      301 tgtaggcgcg gcccccctgg tccagacccg cgcgctggtg aagcgccatt acgcccgcgg
      361 gcttctggcg cgcggtggat ccggaacgct ggccgtggat cgggtgagcc tggagatcga
      421 acgggcgcgc gtctttgggc tggtgggaga gagcggctgc ggaaagagca cgctggcccg
      481 ggccctggtg tggctggatc cgccgacctc gggggacgtc ttctacgacg gtgtctctct
      541 cgggaccttg agccgtcgag gtctccgcgc cctgcggacg cgcatgcaga tcgtctttca
      601 ggatcccaac ggcgcgctgg atccccgtat gcgcgtgcgc cagagcatgg aggagggtct
      661 ggtcaccatc ggcatgccgg ccgccgagcg ggagcgccgg gtggacgagc tcctggatct
      721 ggtcggcgtc tctcccgaaa gcgcgtcacg ctacccgcac gagttctccg gcggccagaa
      781 gcagcgcatc gtcgtggccc gcgcgctcac cctggcaccg gagttcctgg tcctggacga
      841 gccggtctcc aacctcgacg tgtccgtcca ggcgcagatc atcaacctcc tgatggacct
      901 caaggagcgg ttctcgctga cctacctctt catcacgcac gacctccacc tggtcgccta
      961 cctgagtgac cgcatcgggg tgatgctccg cgggcgcctg gtggagaacg cgtcggccga
     1021 ggagctcctg tcccatccgc tgcaccccta caccgtgcgg ctctttgcgt cggtgccggg
     1081 agtcgatgca tcgcatcccc cgcagcccgg caggctggcg gatgccgcgg aatccgttcg
     1141 ggccggctgc ccgtttcggc catcctgcga ccgcgccctg tcgatatgcg cgatcgagga
     1201 cccggagctg cgggacgtcg gggccgggca tcttgctgcc tgccatgcgc tggtatagaa
     1261 tctcgccaac ccccgcggcc actatggagg aaccggatga acaggaacct gtgcgctgcc
     1321 cttctggcct gcctgctgct cctaccaggt ggagctctgt cggcacgaga caaggccctc
     1381 aacttcgccc tctccggaaa cccggatacg ctcgatccgc agaagacctc ggggaccctg
     1441 acgttccaga cgctgaagag catctatgac acgctggtcg agccggacca gaccggcgcc
     1501 atcgtgccgg cgctggctga gagctggacc gtctccgccg acaacctgac ctggacgttc
     1561 aagctccgac ccggggtctt tttccacaac ggggaccgac tcgattcggc ggacgtcaag
     1621 gcaacgctgg atcgcatgcg cgacaaggcc accgcgtcgc ccaaggtcgt cgagttcacg
     1681 acgatcgctt cgatcgacac gccggacgat gccacggtgg tcatccggct gagcaaaccg
     1741 tcggcgccgt tcctggctac gctggcttcc ggatgggctg ccatcctgcc gaagagcctc
     1801 atcgactccg ggaacgactt cgccactcgg ccggtcggga ccggtccgtt catgctcaag
     1861 gagttcgtcc gggacagccg gatcgtcctg gtgaagaatc cgcggtactg gatgccgggg
     1921 ctgccccggc tggaccaggt gatcatgaac atcatcccgg agcgttcggt ccaggtgcag
     1981 gggctgctca ccggcgccat cgacgccgtg gagttcgtga acaccgatga catccagctc
     2041 ctggcaagct cgtcggacgt gaagatcgac aagagcctca ccgcggacat catggtgatg
     2101 tccatgaact gctccaaccc catcctctcc gatcttcgcg tgcgccaggc ggtcacctac
     2161 gccatcgaca agcagaaggt gctcgacatc gcctacggag gcggcaaggt catcgggacc
     2221 ttcatggacg cggccaacgc ctactacaag gacttcacga gcctctaccc ctacaacccg
     2281 gacaaggccc gaagcctcct caaggaggcg ggcgttccag cggacgccgt gttcaccatg
     2341 gtgctgccgt ccaactacga cctccacgta aaggcgggcc agctctacca ggaaatgctg
     2401 agcaacgtcg gtctcaaggt gaacatcaag atggtcgact ggcctacctg gatttccgac
     2461 gtgtacacga acgccaggta cgatttcacc gtcatcgggc ataccgggaa gctggatccg
     2521 gacggcaccc tgagcggata tggcggcaag tcgcgctacg tgcgatggga caaccccgcg
     2581 gcgttggacc tcatcacccg ggccgccggg gtcacggggt tcgatgcgcg gaagaagctc
     2641 tatgaccagg cgctggagat catggcgcga gaggtgccct tcgtatacct ggggagcgcc
     2701 taccgctatc gcgcctatcg atccaacgta tctgagtatc ggatgacccc gaagctcgac
     2761 acctatgatt tccgctggac cgacctgaag tgaagcgttc cggccgccgc ggcggcagcc
     2821 tggcctatcg gctggcgctt gcttatctgt tgatcctggg gggagccgcc gtcgcggcgc
     2881 cgtggatcgc tccgcacagc cccaccgctc agaacctgtc ccagaggttc gctccgccgt
     2941 cccgcgacca tctcctgggg acggacgact tcggcaggga cgtcctcagt cggatcatcc
     3001 atgggtcccg ggctgccctg ctggtgggcg tcgtctccgt ggccatcgcc gttgtcctgg
     3061 gcatgaccgc gggactgctc gccgcgcttg cgccgggatt cgtcgacaac gcggtgatgc
     3121 tggtgatgga cagcctgctt tcgtttccca ccgtgctcct cgccatcacc gtggtgtcct
     3181 tcctggggta cggcttggtc ccggtgatga tcgccatcgg cgtgatctac agtcctacgt
     3241 tcgcccgact cgtgcgagcg gaggccctgg tgatcaaggc cgaggagtac gtcgacgcgt
     3301 cgcgctccct gggcagcgga ccgctcaagg tggcgatgag gcacatcgtg cccaacctgt
     3361 ccggcaaggt gatcgtgcag gcatccgtga ccttcgccct ggcggttgtc gtggaggcct
     3421 ctctgtcgta cctggggttg ggcacccagc caccggcggc gagctgggga ctcatgctga
     3481 aggatgccag gaactttctg gtccaggcgc cctggatggc gctgtacccc ggcctggtcc
     3541 ttgcgctgac cgtcctgagc ttcaacgtga tcggggatag gctggccgag cggctgaatc
     3601 ctaaactggg gtagcggatc ccaacggccc gggctagatg atccccagct tcttccccgc
     3661 ctcacccgcg acctccagga tgtggtcgca ctcctggtcc gtgtggatgg ccatgtagga
     3721 ggtccggatc agcgtgctgt cgggcggcac ggccgggctg agcaccgggt tggtgaacac
     3781 gccgccatcg aacagggccc gccagaagag cagggttttc tccatgtctc cgatgaggag
     3841 cgggacgatg ggggtctcgg cggttccgac gtcgaagccc agcgccttga acccgctcac
     3901 catcttgcag ccgatgctct gaagccggtt cacgcgctcc ggctcctgct cgatgatgtc
     3961 caggcaggcg cgcacggagg cgatggtagc cgggggcatg gacgcgctga agatcagcgg
     4021 ccgcgccatg tgcttgatgt agtcgatcac atcctcgtcc ccgg
//
LOCUS       DASRZS010000515         7087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2197476, whole genome shotgun sequence.
ACCESSION   DASRZS010000515 DASRZS010000000
VERSION     DASRZS010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2197476"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VFH83_12590"
     CDS             <1..686
                     /locus_tag="VFH83_12590"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HET6487256.1"
                     /translation="DIELIDSALEHNSMAAASIHDLRMDHGTLDRSSFSGTKLKDVMI
                     RGQSSILASDFSGCGVSNLRLEGESGGSSLKGSRLSGARVSDVLLRSSTIEDADFSGS
                     SLHDLKMMDSGLVGCKFRGSSLHNVEIASSHLDGCRMDGVGLDDVKVEGSRLVKVQFL
                     GAMDHFRRQVHDVQIRDSTLDGVVFDGCVLQGTRIEGVTASGLRLRGKDLSRMTITSA
                     EALEELAQK"
     gene            729..1739
                     /locus_tag="VFH83_12595"
     CDS             729..1739
                     /locus_tag="VFH83_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6487257.1"
                     /translation="MDIIDVQGLRKSFRSRKGKKGPEIVEAVKGINLWVAKGEIFGFL
                     GPNGAGKSTTLKMLCTLLPPDGGSAFIAGFDLATQAKRVRREIGYVSQAGGADQSATG
                     REDLLLQAGLFGLSAADATRRAEELITALELGPFVDRFTRTYSGGQRRKLDIALGMVH
                     RPAVLFLDEPTTGLDPVSRAQLWEQIRRLREQGTTVFLTTHYMDEADLLCDRLAIIDK
                     GTIVTEGTPAELKRRISGDILTLSVNHVENAMELSRQALAHLEFVKESQAADGRLTLV
                     VSNGGEAIPVVLRTLESRGIGIADISLSRPSLDDVFLRMTGHSLGETAPRGATTLMVA
                     KE"
     gene            1744..2505
                     /locus_tag="VFH83_12600"
     CDS             1744..2505
                     /locus_tag="VFH83_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487258.1"
                     /translation="MRFVKEFRLLFVRSVFGSLRNPVWLFLGLFQPALYLLLFAPLLG
                     GLGNVPGFPPGGAYAVFTPGLLVMVALYSTAFAGFGLVDQIRSGLVERLLVTPASRMA
                     ILLGYVVRDVVTLLVQAGILIGLALIMGLKVSLPGLLVSFAMVVLTGTLMASCSYALA
                     LLLRREDSLASVINTVATPLLLLSGITLPLSLAPKIIRSIASVNPLSYEVTAGRALFQ
                     GNFTDSAIVVGILFLVGLTALSFVWAARSFRRGAA"
     gene            2586..5348
                     /gene="ppc"
                     /locus_tag="VFH83_12605"
     CDS             2586..5348
                     /gene="ppc"
                     /locus_tag="VFH83_12605"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET6487259.1"
                     /translation="MEKTQKLGAEIRLLGRVLGEVIREQAGQSLFELEEEIRLGARAR
                     RDGKPDAERALVSRIRSMSDLEARTVVRAFTLFFDLVNLAEDRERVRVLRERERRRYP
                     EPRSESMEDGVLLMSRAGLDPARSQALLDLLSIRLVFTAHPTEAKRRSVRAKVRFLRQ
                     TLQAMDTEDLLPGERERLVTRVRSLLTGLWQTDLVRPRRPSVLEEVEVGLYFLATLWE
                     ITPVLYAELERSLAKIHPGVSFHPPAFLTFGSWIGGDRDGNPGVTAQVTTATLQRMRR
                     SAVEAHLAQCRLVFDELTSSEREIGTTPELRKLLQERLGRFPEAAALLDPISPFETYR
                     RFLQVVEWRLTQTLDPEPASPSARAARPGGAYANGDELAADLVLVRDSLMANRGSHIC
                     EGELQRWIWQVRVFGLYFARLDIRQESTVNARVVARLLSDAGVTPDYMALPEEERLRV
                     LHQVLASGLELPASSDASWPDEVRETLALFAALRSVAAGQGEDALGGYIISMTHRVSD
                     VLAVFLLWTAGAGAAGQAMPAMDIVPLFETIRDLENAARILGDILDDPIYRRQLAARG
                     NVQVVMIGYSDSTKDGGYLPACWELYQAQGVLYRVARERGVRLIFFHGRGGSLGRGGG
                     PAARSILALPPETLEAGLRITEQGEVLADRYDDPNIAMRHLEQVIWATLAASVRPLSP
                     PRQQWLDAMQELARRALAVYRRLIEQPGFMEFFEQFTPIREIESLPIASRPAHRTGVK
                     SFSDLRAIPWVFAWTQNRCMIPAWFGVGTASFELAEARPDGWDLLREMYQSWPFFHAT
                     LDNAVLALAKADLDMARRYSELVQDAGLRERIWQPIASEYTRSREAVLRINGQRELLD
                     EVPWLKESIRVRNPNTDPLNLIQVEWLRRLHEAERLQDQKRQAEYRELLRLTIEGVAA
                     GMRTTG"
     gene            5369..>7087
                     /locus_tag="VFH83_12610"
     CDS             5369..>7087
                     /locus_tag="VFH83_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487260.1"
                     /translation="MTLARDPMFCCVIMYPTDRELAMGLSDIHLGSFSEKSFQGFPLR
                     AAKVVRQDRPAAQIAADLASCGFTAALLVGFCGSNFTPEDIRSARFVRLTKALAARGM
                     TVGVCIPFADTVDNQLTRADPRMHQLRHDGKRPYIRFDPEITRPPYALYTIDYGSESF
                     IRFAQEIVLAARDAGMSFIDFAEPDYWPTEGNGYGSYLEEAWRKRYKEPLPYPSDPRH
                     RLFMEDYHLESLRRITAFAHEHGIRTHLTASPLGHAPYHICQNYGKYSTTEITELSST
                     YHHTYGLAWRDRIQRRYGVRFGDHRLSTIGCLEARGMRGWDERHATYLVPGQSLPAET
                     CANALDQQMFLHNMDVVFWEYPSIRTNYYETDDPWFTGEKAWHALRDLFRQRSEQYRA
                     LPATFHRAHHFPEALVYFSKRGVYAEEAEDRTTPCAYATALKLQSENVLPAFLFAERL
                     APLESTRHEARLLLMDEHHPVSMQGWTRIRRWLGRGRRVVVYGGEPGWRWEMDGPRKG
                     FAPEMERLFGVQKGGTLIPGGRVVPAPPLDPLPQGMQRVEVSARRGVRTLQSADGRPF
                     VYLKRLS"
BASE COUNT         1099 a         2389 c         2435 g         1164 t
ORIGIN      
        1 acgacatcga gctcatcgac agcgccctgg agcacaactc catggcggcg gcgtccatcc
       61 atgacctgcg catggatcac gggacgctgg accgaagctc cttttccggc accaagctca
      121 aggacgtcat gatccgaggt cagtcctcga tcctggcgag tgacttctcc ggttgcggtg
      181 tctcgaacct caggctggaa ggagagtccg gcgggtcgtc cctcaagggc tcccgtctgt
      241 cgggcgcccg agtctccgac gtccttctga ggagcagcac catcgaggac gccgatttct
      301 ccggcagctc gctccacgat ctgaagatga tggacagcgg gctggtcggc tgcaagttcc
      361 gcggctccag cttgcacaac gtggagatcg cctcctccca cctggacggg tgccggatgg
      421 acggtgtcgg tctggacgat gtcaaggtgg agggctccag gctcgtcaag gtacagtttc
      481 tgggcgccat ggaccatttc cgcaggcagg tgcacgacgt tcagattcgc gattcgaccc
      541 tggatggcgt ggtcttcgac ggctgcgtcc tccagggcac caggatcgaa ggggtcaccg
      601 catcggggct tcggctccgc ggcaaggatc tctcgagaat gaccatcacc agcgccgagg
      661 cgctggagga actggcccag aaataggccg ggccgttccc tcatagctcc aaaggagagg
      721 agatactcat ggacatcatc gacgtccaag gtctgcgcaa gagctttcgc tcccggaagg
      781 gaaagaaggg gcccgagatc gtggaagcgg tcaagggaat caacctctgg gtcgcgaagg
      841 gagagatctt cggtttcctg ggcccgaacg gtgccgggaa gagcaccacg ctcaagatgc
      901 tttgcaccct tctgccgccg gacggcggct cggcgttcat cgcggggttc gacctggcca
      961 cgcaggcaaa gagggtgcgc cgcgagatcg gctacgtgag ccaggccggc ggcgcggacc
     1021 agtccgccac cggccgcgag gacctgctcc tgcaggcagg gctcttcggc ctttcggccg
     1081 cggatgccac gaggagagcg gaggagctca tcaccgccct ggagctcgga ccgttcgtgg
     1141 atcggttcac gcgaacgtac tccggggggc agcggcgcaa gctcgacatc gccctgggca
     1201 tggtgcaccg gcccgcggtg ctcttcctgg acgagcccac caccgggctg gacccggtga
     1261 gccgcgccca gctctgggag cagatccgca ggctgcggga gcagggaacc accgtgttcc
     1321 tgaccaccca ctacatggac gaggcagacc tgctctgcga ccggctcgcc atcatcgaca
     1381 agggcacgat cgtgacagag gggacgcccg ccgagctgaa gcgccggatc tcgggggaca
     1441 tcctaacgct gtccgtgaac cacgtggaga acgccatgga gctgtcccga caggcgctgg
     1501 cgcacctgga gttcgtgaag gagagccagg ccgcggacgg ccggctcacc ctggtggtct
     1561 ccaacggcgg ggaggccatc ccggtggtgc tgcggacgct ggagagccgg gggatcggga
     1621 tcgccgacat ctccctctcc cgaccgtccc tggacgacgt gttccttcgg atgaccgggc
     1681 actcgctcgg cgagaccgcg ccgcgcggcg cgaccaccct gatggtggca aaggagtagc
     1741 gtgatgaggt tcgtaaagga gtttcggctt ctgttcgtgc gcagcgtgtt cggttccctg
     1801 cgcaaccccg tttggctctt cctgggcctg ttccagccgg ccctgtacct gcttttgttc
     1861 gcgccccttc tgggcgggct gggcaacgtg cccggattcc ctccgggcgg agcctacgcc
     1921 gtgttcaccc ccgggctcct ggtgatggtg gcgctctaca gcaccgcctt tgccggattc
     1981 gggctggtgg accagatccg aagcgggctg gtggagcggc ttctggtcac gccggccagc
     2041 cggatggcca tcctgctggg ctacgtggtc cgggacgtgg tgacgcttct ggtccaggcc
     2101 ggaatcctga tcgggcttgc cctcatcatg gggctgaagg tcagcctgcc ggggctcctg
     2161 gtcagcttcg ccatggtcgt cttgaccggc acgctgatgg cctcctgctc ctacgcgctg
     2221 gctctgctcc tgcgccggga ggactctctg gcgtcggtca tcaataccgt cgcgaccccc
     2281 ctgctgcttc tctcggggat cacgctgccg ctgagcctcg cgccgaagat catccggtcc
     2341 atcgcgtcgg tgaaccctct gtcgtacgag gtgaccgccg ggcgcgctct gttccagggc
     2401 aacttcacgg actccgccat cgtggtaggg atcctgttcc tggtcggtct gacggccctg
     2461 tcgttcgtat gggccgcgag gtcgttccgt cggggcgccg catgatggca accgcgggcg
     2521 gtcggccgtc cggcggcggt ccggcggctg acaagcccgc tgctctttga catgatgggc
     2581 ccagcatgga gaagacccag aagctgggcg cggagatccg gctcctcggc cgcgtgttgg
     2641 gggaggtgat ccgcgagcag gcaggacaga gcctcttcga gctggaagag gagatccgcc
     2701 tgggggcgag ggcgcgccgc gacgggaagc cggacgcgga gcgcgccctg gtctcccgca
     2761 tccgctcgat gagcgacctg gaagcccgga ccgtggttcg ggctttcacg cttttcttcg
     2821 acctggtcaa cctggcggag gatcgcgagc gggtgcgcgt cctgcgggag cgggagcggc
     2881 gccggtatcc cgagccgcgc tccgagtcca tggaggacgg cgtcctcctc atgagccgcg
     2941 cggggctgga tccggcccga agccaggccc tcctggatct cctctccatc cggctggtct
     3001 tcaccgccca ccccacggag gccaagcgac ggtcggtccg cgcgaaggtc cggttccttc
     3061 gccagaccct gcaggccatg gacaccgagg atctgctgcc cggggaacgg gagcgcctgg
     3121 tgacgcgggt ccgatcgctg ctcaccggcc tgtggcagac ggacctggtc cgtccccgcc
     3181 gccccagcgt gctggaggaa gtggaggtgg ggctctactt cctggccacc ctgtgggaga
     3241 tcaccccggt tctctacgcg gagctggagc gctcccttgc gaagattcat cccggggtct
     3301 cgttccatcc tcccgccttc ctgacgttcg gctcctggat cggaggagac cgggacggca
     3361 accccggcgt gaccgcgcag gtgaccacgg ccacgctcca gcggatgcgc cgaagcgcgg
     3421 tggaggccca cctggcgcag tgccggctgg tcttcgacga gctcacctcc tccgagcggg
     3481 agatcggcac cacgccggag ctgcggaagc tgctccagga gcggctgggg cggttccccg
     3541 aggcagccgc gctcctggac ccgatctcgc ccttcgagac ctaccggcgg ttcctgcagg
     3601 tggtggagtg gcgcctcacg cagaccctgg acccggagcc cgcatcgccg agcgcccggg
     3661 ccgcgcgtcc cggcggagcc tacgcgaacg gagatgagct ggccgccgac ctggtcctgg
     3721 tgcgggacag cctcatggcg aatcgaggct cccacatctg cgagggcgag ctgcagcgct
     3781 ggatctggca ggtccgggtg ttcgggctgt acttcgcccg gctggacatc cggcaggaat
     3841 cgaccgtgaa cgcgcgggtc gtggcacggc tcctctcgga cgccggagtc acgccggact
     3901 acatggctct ccccgaggag gagcggctgc gggtcctgca ccaggtgctg gcctccggcc
     3961 tggagctacc ggcctcctcc gacgcctcct ggcccgatga ggtccgggag acgctggcgc
     4021 tctttgccgc gcttcgctcg gtggcggccg gccagggaga ggatgcgctg ggcggctaca
     4081 tcatcagcat gacccatcgg gtaagtgacg tgctggccgt gtttctcctc tggacggccg
     4141 gcgcgggtgc cgccggccag gccatgccgg ccatggacat cgtcccgttg ttcgagacca
     4201 tccgggacct cgagaacgcc gcccggatcc tgggagacat cctggacgat ccgatctacc
     4261 gacgccagct cgccgctcga gggaacgtcc aggtcgtgat gatcggctac tccgacagca
     4321 ccaaggacgg aggctacctg ccggcgtgct gggagctgta ccaggcccag ggggtgctgt
     4381 accgcgtggc ccgcgagcgc ggcgtgaggc tgatcttctt ccacggccgc ggcggatcgc
     4441 tgggccgcgg cggcggcccg gcggcgcgca gcatcctggc gcttccgccg gagaccctgg
     4501 aggccgggct tcgcatcacg gagcagggtg aggtcctggc ggaccgctat gacgacccca
     4561 acatcgccat gcggcacctg gagcaggtga tctgggctac ccttgccgcc tcggtccgcc
     4621 cgctctcccc gccaaggcag cagtggctcg atgccatgca ggagctggcc cggcgggccc
     4681 tggcggtcta tcgccgcctc atcgagcagc cggggttcat ggagttcttc gagcagttca
     4741 cgccgatccg cgagatcgag tccctgccca tcgcctcccg gccggcgcac cgcaccggcg
     4801 tcaagtcgtt ctccgacctg cgcgccatcc cgtgggtgtt tgcctggacg cagaaccgct
     4861 gcatgatccc cgcctggttc ggcgtgggca ccgcctcctt cgagctggcg gaggcgcgcc
     4921 ccgacgggtg ggacctcctt cgggagatgt accagagctg gccgttcttc cacgcaaccc
     4981 tggacaacgc cgtcctggcc cttgccaagg ctgacctgga catggcgcgg cggtactcgg
     5041 agttggtgca ggacgcgggt ctccgggagc gcatctggca gcccatcgcg tcggagtaca
     5101 cccgcagtcg ggaggccgtg ctccggatca acggccagcg cgagcttctg gacgaggtcc
     5161 cgtggctgaa ggagtccatc cgggtccgaa accccaacac cgaccccctg aacctgatcc
     5221 aggtggaatg gctgcgaagg ctgcacgagg ccgagcgcct ccaggaccag aagaggcagg
     5281 cggaataccg cgagctcctc cgcttgacca tcgagggagt cgccgcgggg atgcggacca
     5341 cggggtgagg tcaaaaaggc ggcgtccggt gaccctggcc cgggacccga tgttctgctg
     5401 cgtcatcatg taccccacgg accgcgagct cgcgatgggg ctctccgaca tccacctggg
     5461 gtcgttctcg gagaagagct tccaggggtt tcctttgcgg gcggcaaagg tggtgcgcca
     5521 ggaccggccg gcagcccaga tcgcggcgga cctggcttcc tgcggcttca ccgcggcgct
     5581 tctcgtgggg ttctgcggaa gcaacttcac gccagaggac atccgctccg cccggttcgt
     5641 ccggctcacc aaggcgctgg cggcgcgcgg gatgaccgta ggagtgtgca tccccttcgc
     5701 ggataccgtg gacaaccagc tcacgcgggc cgacccccgc atgcaccagc tccgccacga
     5761 cggcaagcgc ccatacatcc gcttcgatcc cgagatcacg cggcccccgt acgcgctgta
     5821 cacgatcgac tacggctccg agagcttcat ccggtttgcc caggagatcg tccttgcggc
     5881 gcgggacgcg ggaatgagct tcatcgactt cgcggagccg gactactggc cgaccgaagg
     5941 caacggatac ggaagctatc tcgaggaggc gtggcgcaaa cgctacaagg agccgctgcc
     6001 gtacccgagc gatccgaggc accggctctt catggaggat tatcacctgg agagccttcg
     6061 gcggatcacc gcgttcgcgc acgagcacgg gatccgcacg catctcaccg cgagcccgct
     6121 ggggcacgcg ccgtaccaca tctgccagaa ctatggcaag tactccacca cggagatcac
     6181 ggagctctcc agcacctacc accataccta cgggctggcg tggcgggatc ggatccagag
     6241 gcgctacggc gtgcgcttcg gggaccaccg gctctctacc atcggctgcc tggaggccag
     6301 gggcatgcgg ggctgggacg agcggcacgc cacctacctg gtccccggcc agagcctgcc
     6361 cgcggaaacc tgcgccaacg ccctggacca gcagatgttt ctccacaaca tggacgtggt
     6421 gttctgggag taccccagca tccggacgaa ctactacgag acggacgatc cctggttcac
     6481 gggggagaag gcctggcacg cgctgcggga cctctttcgg cagcggtccg agcagtatcg
     6541 ggccttgccc gccacgttcc atcgcgccca tcacttcccg gaggcgctcg tctacttctc
     6601 caagcgcggg gtctacgccg aggaggcgga ggaccggacc acgccgtgcg cgtacgccac
     6661 ggcgctcaag ctccagtcgg agaacgtcct gcccgcgttc ctcttcgccg agcggctggc
     6721 cccgctggag agcacccggc acgaggcgcg gctccttctc atggacgagc accacccggt
     6781 gtcgatgcag gggtggaccc ggatccgccg gtggctgggt cgggggagac gcgtcgtggt
     6841 ctatggggga gagcccggat ggcgttggga gatggacgga ccccgcaaag ggttcgctcc
     6901 ggagatggag cgtctcttcg gagtccagaa gggcggcacg ctcatccccg gcggccgggt
     6961 tgtcccggct ccgccgctgg atcctcttcc ccagggcatg cagcgggtcg aggtgagcgc
     7021 cagacggggc gtgcgcacgc tccagtccgc tgacggcagg cccttcgtgt acttgaagag
     7081 gctgtca
//
LOCUS       DASRZS010000516         5298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5670814, whole genome shotgun sequence.
ACCESSION   DASRZS010000516 DASRZS010000000
VERSION     DASRZS010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5670814"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VFH83_12615"
     CDS             complement(<1..1181)
                     /locus_tag="VFH83_12615"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HET6487261.1"
                     /translation="MAGFPNGFLWGAATAAYQVEGASREDGKGESIWDRFCERPGAIH
                     GGDTGDVACDHYHRWREDVTLMHALGLKSYRFSVSWPRVFSNGRGKPNRKGMDFYQGL
                     VDGLLANGIEPALTLYHWDLPQALQDHGGWTNRDTASWFADYAAAVYQALGDRVKLWM
                     TLNEPQVSAFAGYVDGAHAPGLRDLSAGLRASVVLHRAHALAVQAFRGMSLGRTARVG
                     IALDLHPVWPVGDGHADEDAARIADGVQNRWFLDPVFTGRYPADILEHYAAAKVAPEI
                     PNEDLGLLEAFPPDFLGVNYYFPVRVRPRKNALGFEGVVPSDREQTAMGWEVNPAALR
                     ELLLRLRKDYGDPVLFITENGAAYDDRPDADGRIQDDRRIAYLDSHLQEARRAIDDGV
                     RL"
     gene            complement(1248..2126)
                     /locus_tag="VFH83_12620"
     CDS             complement(1248..2126)
                     /locus_tag="VFH83_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6487262.1"
                     /translation="MHLQYRRARVFDVVIRCVLVLLSFLLLYPFIYCLSYSFSNSREA
                     MITNVVLLPVAPTLENFSYVFANNAIVHSFVISVLRTVSGILWALLVTGLAAYAISKK
                     TLPFKRTLSIFFVIPMYISGGLLPTYVWIHDLHLFNNFLVYILPHGFWAFNMLLMRSY
                     FDTIPESLEEASRIDGAKDLRILVQVVVPLSMPIIAVIAVYVGVWHWNEWFEALLYIT
                     HPWLKPMQSMLQRIIMESMGSAMEAQSGRVTEHEVSPTSIQMATLIVSTVPIILIYPF
                     VQRYFIQGVMIGAVKA"
     gene            complement(2139..3083)
                     /locus_tag="VFH83_12625"
     CDS             complement(2139..3083)
                     /locus_tag="VFH83_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6487263.1"
                     /translation="MRPNAAALAPRTTWKEVVRHRTLYLFLLPAVVLCLVFFYRPMIG
                     LIMAFQNYKVALGMYHSPFVGLKNFQEFLQNPRFYQTLRNTLVINGLSIAIGFPLPII
                     FALVLNTLKDGSFKKVTQTISYLPHFISWVVIAGLMYKLLDQESGAVNLLIKLMGGKP
                     VAYMRQAQSFWGVITVVAIWKELGWNTIIFLAALASVDVEQYEAAIIDGASGWQKLWG
                     ITLPAIAPVIALMFIFTIGNLFNANGNVSFDAIFNMRNALISDTANTIDYFIYQEGVQ
                     SNRMSFAAAIGFAQNLISFIVVMGANAISRKIRGYGAF"
     gene            complement(3163..4848)
                     /locus_tag="VFH83_12630"
     CDS             complement(3163..4848)
                     /locus_tag="VFH83_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487264.1"
                     /translation="MNRRSYLSIALLLAGAMFLAGSFAFAADPLMTYTAMPRMPESEK
                     YINNPHDVVTPWIENKFNIKVSDVIIASATIQFKQRYNQMAAADDVPDVITSGKGDTD
                     YAVASGLYADLTDYIKDMKNYNKYFDPKYLPMYLNDGRQYQISDIELNLNDPKYDADP
                     YNMGAPNWCLWAREDILAKAGYKFTPMADLQKRAAATGKKPTAAEMAITPAIDTPAKF
                     VDLLRKIKALNLKVGDRSVIPLSVTSWQQFHLGCQFAFGHWHKYDDGTVAGFLESPGT
                     KDWYHDLWQINKEGLIDPDWLVQKEEQLQEKASAGLVAVGMYLSDIPAARANMQKVNS
                     AYQIRYIPWPKTNPNQGFFDVYVGGFERTIISKKVKDIKRLTQYFDWFFSDEGLDMLT
                     WGPANTGVWIMDHGKKVFTKDVEQDMLSSKKGAKGPDYWGLYDYTSYILPYLSKAGLC
                     AAKLMVGNPATYLKSYPVNIDVFTYARSLYGRAGMDYSGKGTYGDGGDNDSNWQNYFW
                     GKWQNDRCAKLLGAKDENDFNQIFDQSRQLWLSEGNYDAAHADLVKWFNANTN"
     gene            complement(5032..>5298)
                     /locus_tag="VFH83_12635"
     CDS             complement(5032..>5298)
                     /locus_tag="VFH83_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487265.1"
                     /translation="GFAPAVKDTKGASEFLKVYTDQMANYGTERIEPFDSMLTREFLD
                     EYYSDLQDFLMGKTPLDQTATLIQASMMKAAKQLLQENPDWAEK"
BASE COUNT          952 a         1589 c         1768 g          989 t
ORIGIN      
        1 agccgaacgc catcgtcgat ggcgcgccgc gcctcctgca ggtgcgagtc caggtaggcg
       61 atcctgcgat catcctggat ccgtccgtcc gcgtccggcc ggtcatcgta ggccgcgccg
      121 ttttcggtga tgaagagcac cgggtcaccg tagtccttgc gaagccgcag aaggagctcc
      181 cgaagcgcgg ccggattcac ctcccagccc atggccgtct gctcacggtc cgaggggacc
      241 acgccctcga agcccagggc attcttccgg ggccggaccc gaaccgggaa gtagtagttc
      301 acaccgagga agtcgggcgg gaaggcctcg aggagcccca ggtcctcgtt ggggatctcc
      361 ggggcgactt ttgcagccgc gtagtgctcc aggatgtcgg cggggtatct gccggtgaac
      421 acggggtcca ggaaccagcg gttctggacg ccgtcggcga tccgggcggc gtcctcatcg
      481 gcgtgaccgt cccccacggg ccagacgggg tggagatcca gggcgatgcc gacccgcgcc
      541 gtcctgccca gggacatgcc gcggaaggcc tggaccgcca gcgcgtgggc ccggtgcagg
      601 accacggagg cgcggagtcc ggccgacagg tcgcgcagac ccggcgcgtg cgcgccatcc
      661 acgtagccgg cgaacgcgga tacctggggc tcgttcagcg tcatccagag cttcacccgg
      721 tccccgagcg cctggtagac cgccgcggca tagtccgcga accacgacgc ggtatcccgg
      781 ttggtccaac cgccgtggtc ctggagcgcc tgcggcaggt cccagtggta gagggtaagc
      841 gccggctcga tcccgttggc cagcaggcca tccacaaggc cctggtagaa gtccatcccc
      901 ttgcggttcg gcttcccgcg gccgttggag aaaaccctgg gccaggagac ggagaatcga
      961 tacgacttca gcccgagggc gtgcatgagg gtcacgtcct cccgccagcg gtggtagtga
     1021 tcgcaggcaa cgtccccggt atccccgccg tggatggcgc cggggcgctc gcagaaccgg
     1081 tcccagatgg actccccctt gccgtcctct cgggaagctc cctcgacctg ataggcggcg
     1141 gtcgccgctc cccacagaaa cccgttcgga aacccagcca tgcgggacct cccggcgcgt
     1201 cggcgcctgt atccgacgtt ggcgcctcgg ttcggtgctc ggcgcctcta tgccttgacc
     1261 gctccgatca tgaccccctg gatgaagtag cgctgcacga aggggtagat caaaatgatc
     1321 ggcaccgtgc tcacgatcag cgtggccatc tgaatggaag tcggagagac ctcgtgctcg
     1381 gtgactcgcc ccgactgcgc ctccatggcg cttcccatcg actccatgat aatccgctgc
     1441 agcatcgatt gcatcggttt gagccagggg tgggtgatgt acaggagcgc ctcgaaccat
     1501 tcgttccagt gccagacccc gacgtagacc gcgatcacgg cgatgatggg catggacagg
     1561 ggaacaacga cctggaccag gatgcgaagg tccttggctc cgtcgatccg cgatgcctcc
     1621 tcgaggctct ccgggatggt gtcgaagtag gaccgcatga ggagcatgtt gaacgcccag
     1681 aacccgtgcg ggaggatgta caccaggaag ttgttgaaga ggtgcaggtc gtggatccat
     1741 acgtacgtgg ggagcagccc gccgctgatg tacatcggga tgacgaagaa gatggagagg
     1801 gtgcgcttga aggggagcgt cttcttggag atggcatagg cagccaaccc ggtgaccagg
     1861 agcgcccaca ggatccccga cacggttcgc aggacggaga tcacgaacga gtggacgatc
     1921 gcgttgttgg cgaacacgta ggagaagttc tccagggtag gcgctaccgg cagaagcacc
     1981 acgttggtga tcatcgcctc ccggctgttg ctgaaggaat aggagaggca gtagatgaac
     2041 ggatagagca ggaggaagga caggagcacc aggacgcacc ggatcacgac gtcgaacacc
     2101 ctggccctgc gatactggag atgcatcgtc ggccccctct agaacgcgcc gtagccgcgg
     2161 atcttgcggg agatcgcgtt ggcgcccatg acgacgatga aggagatcag gttctgggcg
     2221 aacccgatgg ccgcggcaaa ggacatccgg ttggactgca cgccctcctg gtagatgaag
     2281 tagtcaatgg tgttcgccgt atcggagatc agcgcgtttc tcatgttgaa gatcgcgtcg
     2341 aaggagacgt tcccgttcgc gttgaacagg ttcccgatgg tgaagatgaa catcagggcg
     2401 atgacggggg cgatggccgg tagcgtgatg ccccagagct tctgccagcc ggaggcgccg
     2461 tcgatgatgg ccgcctcgta ctgctccacg tcaacgctcg ccagggccgc caggaagatg
     2521 atggtgttcc agcccagctc cttccagatg gccaccaccg tgatgacccc ccagaaggac
     2581 tgggcctggc gcatgtaggc gacgggcttc ccgcccatca gcttgatgag caggttgacc
     2641 gcgccggact cctggtccag gagcttgtac atcagccccg cgatgacgac ccaggagatg
     2701 aagtggggca ggtagctgat ggtctgggtg accttcttga aggagccgtc cttcagcgtg
     2761 ttcaggacca gggcgaagat gatggggagc ggaaacccga tggcgatgga gagcccgttg
     2821 atcaccaggg tgttccggag ggtctggtag aaccgggggt tctggaggaa ctcctggaag
     2881 ttcttcagcc cgacgaaggg gctgtgatac atgccgagcg ccactttgta gttctggaag
     2941 gccatgatca gcccgatcat gggccggtag aaaaagacca ggcacagcac gacggctgga
     3001 agcaggaaca ggtacagcgt ccggtgtcgg accacctcct tccaggtggt cctcggcgcg
     3061 agcgcggcgg cattcggcct catcgccaac tcctagagaa gagaagggcg gccgctcgtc
     3121 cgtccacaga ctcggccgcc cgatacaccc tgcaacagca gcctagttcg tgttcgcgtt
     3181 gaaccacttc accaggtcgg cgtgagccgc gtcgtagttg ccctcggaga gccacagctg
     3241 ccgcgactgg tcgaagatct gattgaagtc gttctcgtcc ttcgcgccga ggagcttggc
     3301 gcagcggtcg ttctgccact tgccccagaa gtagttctgc cagttcgagt cgttgtcacc
     3361 gccgtcgccg taggtgccct tgccggagta gtccatcccg gcgcggccgt acagcgagcg
     3421 ggcgtacgtg aacacgtcga tgttcacggg gtagctcttc aggtaggtgg cggggtttcc
     3481 gaccatgagc ttcgccgcgc agaggccggc cttgctcagg tacgggagga tgtacgaggt
     3541 gtagtcgtac agtccccaat agtcgggtcc cttggcgccc ttcttggagc tgagcatgtc
     3601 ctgctcgacg tccttggtga acaccttctt gccgtgatcc atgatccaga cgccggtgtt
     3661 ggcggggccc caggtcagca tgtccaggcc ttcgtcggag aagaaccagt cgaagtactg
     3721 ggtcaggcgc ttgatgtcct tgaccttctt gctgatgatg gtccgctcga acccgccgac
     3781 gtaaacgtcg aagaagccct ggttggggtt ggtcttgggc caggggatgt agcggatctg
     3841 ataggccgag ttgaccttct gcatgttggc ccgggcggcc gggatgtcgg agaggtacat
     3901 gccgacggca acaagacccg cggaggcctt ctcctggagc tgctcctcct tctggacgag
     3961 ccaatccgga tcgatcaggc cttccttgtt gatctgccac aggtcgtggt accagtcctt
     4021 ggtccccggg gactccagga agccggccac cgtcccgtca tcgtacttgt gccagtgacc
     4081 gaaggcgaac tggcatccca ggtggaactg ctgccaactg gtgaccgaca gggggatgac
     4141 cgagcggtcc ccgaccttca ggttgagggc cttgatcttg cgcaacaggt ccacgaactt
     4201 ggccggcgtg tcgatggccg gcgtgatggc catttcggcc gccgtgggct tcttgccggt
     4261 cgcggccgcg cgcttctgca ggtccgccat gggggtgaac ttgtagccgg ccttggccag
     4321 gatgtcttcc cgggcccaga ggcaccagtt cggcgcgccc atgttgtacg ggtccgcgtc
     4381 gtacttcggg tcgttgaggt tgagctcgat gtcggagatc tggtactggc ggccgtcgtt
     4441 caggtacatg ggcaggtact tcggatcgaa gtacttgttg tagttcttca tgtccttgat
     4501 gtagtccgtc aggtcggcgt aaagcccgga ggccacggcg tagtcggtgt cacccttgcc
     4561 cgaggtgatg acgtcgggaa catcgtccgc ggccgccatc tggttgtagc gctgcttgaa
     4621 ctggatggtg gcggaggcga tgatgacgtc ggataccttg atgttgaact tgttctcgat
     4681 ccacggcgtg acgacgtcat ggggattgtt gatgtacttc tccgactccg gcatgcgcgg
     4741 catggccgtg taggtcataa gggggtccgc cgcgaaggcg aatgaccccg caaggaacat
     4801 cgcgcccgcg agcaggagcg cgatgctcag gtaactcctt ctgttcatga aaacctcccg
     4861 ccagaatagt gtggttcgcc cgtaaccttt ggacaccggg ccgggcgctg ttgagtgagt
     4921 ccgggaactg tagccgagga ccacccgcct gtcaaccaaa aggaacgccg cccaaccgcg
     4981 cgggtgccgg tcgaggggcg gcgttccatg atcctacggt ccgaaccggc tctacttctc
     5041 cgcccagtca gggttctcct ggaggagctg cttggctgcc ttcatcatgg aggcctggat
     5101 gagggtggcg gtctgatcca gcggcgtctt gcccatcagg aagtcctgca gatcgctata
     5161 gtactcgtcc aggaactccc gggtgagcat gctgtcgaag ggctcgatgc gctccgtgcc
     5221 gtagttcgcc atctggtccg tgtagacctt caggaactcg ctggcgccct tggtatcctt
     5281 gactgccggg gcgaagcc
//
LOCUS       DASRZS010000517         4914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2198458, whole genome shotgun sequence.
ACCESSION   DASRZS010000517 DASRZS010000000
VERSION     DASRZS010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198458"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VFH83_12640"
     CDS             complement(<1..950)
                     /locus_tag="VFH83_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487266.1"
                     /translation="MPENLIDIRQLKKYYPVTGGVLRRHLADVKAVDGVDLAIRKGEC
                     LGLVGESGCGKTTLGKTILRLHGATSGRIYFAQKPERIEEIETGLASRDTRVRSKARS
                     QLPSIDLASARGRSLLAMRPKIQIVFQDPTNSLDPRMLVKDIVGEPLVAQRLVKPREL
                     PGRVQELLSIVGLTRDHLMRYPHEFSGGQRQRIAIARALATNPQFVLMDEPTSALDVS
                     VQAQILNLLKRLQQEFDLTYLFVTHHLLVVKYISNRIAVMYLGKICELGPTPEIFSKP
                     LHPYTRALLSAIPVPDPETMQQKIILSGDVPNPIDPPTGCR"
     gene            complement(943..2226)
                     /locus_tag="VFH83_12645"
     CDS             complement(943..2226)
                     /locus_tag="VFH83_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487267.1"
                     /translation="MAQDTLATTQDLFTNFYTFEGVVRALNGVTIVVRHGETYGLVGE
                     SGCGKSVTVRSMMRIVQAPGKIDSGSIFVFLNPEDRLKAIEIVHRSEAYMESIRGCDI
                     SMIFQEPAAALNPVLSIGEQVAESYRFHLNDRMLEETLAGLEGKRGHGLQRALLRREL
                     AAIRRHRARLQRIDNELYPLDGRDDPEAQARKSHLSRMRDRTGGADALVEVARRVPFL
                     RRYRSRLERTVHGHVVELLRSLGIPNPENVAARYPHELSGGMQQRIVIAIALACHPTL
                     LIADEPTSNLDVTIQAQIVDLIKQLKKTAISSVLFITHDLGLVAETCDRVTVMYAGDV
                     AETAIVKELFKHPLHPYTKGLLHSVPKLDQSEELAMIPGAVPNLIAPPTGCRFHPRCP
                     SRMPICVTEKPAVVEHTADHWVACHLYPKGTGSNA"
     gene            complement(2228..3205)
                     /locus_tag="VFH83_12650"
     CDS             complement(2228..3205)
                     /locus_tag="VFH83_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487268.1"
                     /translation="MRTWIPGFDFPEERLLMGTPVQSVSPPKIHPRLDEFLFTARRFI
                     KNPLSVIGLSLILVFGVIALLAPVLEPPKYPDSFKMPHSGWKIEPSPPSAEHPLGTTQ
                     QQYDMLYGMIWGTRSAFKIGVLVVAANLLIGILLGAIAGYFGGAIDEIIMRITDIFYA
                     IPFLVMAMALVVAIGRGLKAVIIVLIILEWPSYTRVLRSDILVTRNKDFILASRASGA
                     SHLRVILTHILPNAMYSVLILASLNIGATILTAAALSFLGLGSDTGVADWGQIVSMGR
                     NWIVGAPGNRFAYWYTVFIPGFVISLFVLGWNLLGDAVRDVFDPKVRRK"
     gene            complement(3171..4205)
                     /locus_tag="VFH83_12655"
     CDS             complement(3171..4205)
                     /locus_tag="VFH83_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487269.1"
                     /translation="MSLLSYIIRRLFLMIFVLIGVSLLVFTVMMLMPPGMRVAAYVTS
                     DRVTPEMMDAMIAKYGLNDPAPVQYIRWIGNLLHGQFGYSITASAPVLTGFMQFFPTT
                     LELVLYAAPLIILVGIFLGTIGAVHKDEPLDHGTRIFAIIGYSLPTFWLGLLLMMILY
                     GFAGLFPPGSLSNSAHEVVNAPGYHQYTHLLTIDAILNWRWDIFWDAFYRLILPTVNL
                     VIINSALIMRLMRSSMLESLGQDYVMTARAKGADSRTLYHKHARRNAMLPVITVSGLT
                     FAGLMSGAVITETIFDRKGIGWWTARAALQLDVAAIMFNVLFLGTVYVVINLIVDLIY
                     AYVDPRVRLS"
     gene            complement(4290..>4914)
                     /locus_tag="VFH83_12660"
     CDS             complement(4290..>4914)
                     /locus_tag="VFH83_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6487270.1"
                     /translation="MDPVTPFPKGLPYKNDKLESKPHDMTKAADYFKKALGGKLWDTG
                     FKFDIVYNTGNTERETGAKILAENVMSLNPKFQVGTRAVEWPVYNEMNKQKQMAVFFL
                     GWAPDYPDPADYAGPYMSSDGFFASRQSYKNPTADDLVAKAAIELDPAKRAAEYSQLQ
                     DIWLQDSIAILAQQPLRRRFMKDWVKGYYYNPMESQEFELLPVLTKK"
BASE COUNT          871 a         1488 c         1686 g          869 t
ORIGIN      
        1 cgacagccgg tgggcggatc gatcgggttg gggacgtcgc cgctcaggat gatcttctgc
       61 tgcatggtct ccgggtccgg cacggggatg gcggagagga gcgcccgcgt atacgggtgc
      121 agcggctttg agaagatctc cggcgtcggg cccaactcgc agatcttgcc cagatacatc
      181 accgcgatgc ggttggagat gtacttcacc accaggaggt ggtgcgtcac gaagagatag
      241 gtgagatcga actcctgctg gaggcgcttc aggaggttga ggatctgcgc ctgcaccgag
      301 acatccaggg cggaggtggg ctcgtccatg aggacgaact gcgggttggt ggccagggcg
      361 cgcgcgatgg cgatcctctg gcgctgtccg ccggagaact cgtgcgggta gcgcatgagg
      421 tggtcccggg tgagacccac gatgctgagc agctcctgga cccggcccgg cagctccctc
      481 ggcttcacca gtctctgcgc gactagcggc tctcccacga tatccttcac gagcatgcgg
      541 ggatccagcg agttggtggg atcctggaac acgatctgga tcttcggtcg catggcgagg
      601 agcgatctcc cgcgggcgct ggccaggtcg atgcttggaa gctgggagcg cgccttggag
      661 cgcacgcggg tgtcccggga ggccagcccg gtttcgatct cctcgatccg ctcgggcttc
      721 tgggcgaagt agatccgacc cgaggtggct ccatgcaggc gcaggatggt cttcccgagc
      781 gtggtcttgc cgcagccgga ctccccgacc aggccaaggc actccccctt gcggatggcg
      841 aggtcaacgc cgtctacggc ctttacgtcc gccaggtggc ggcgcagcac gccgccggtc
      901 accgggtagt acttcttgag ctgtcggatg tcgatgaggt tctcaggcat tggagccggt
      961 ccccttggga tacaggtggc aggccaccca gtggtccgcg gtgtgctcca ccacggccgg
     1021 tttctcggta acgcagatgg gcatgcggct tgggcagcgc gggtggaacc gacaccccgt
     1081 ggggggagcg atcaggttgg gcaccgcgcc cgggatcatc gccagctcct ccgattgatc
     1141 gagcttggga acggagtgga gaagcccctt cgtgtagggg tgcaaggggt gcttgaagag
     1201 ctccttcacg atcgccgtct cggccacgtc tcccgcgtac atcacggtca cgcggtcaca
     1261 ggtctccgcc accagcccca ggtcgtgcgt gatgaaaagc accgaggaga tggcggtctt
     1321 cttgagctgt ttgatgaggt ccacgatctg cgcctggatg gtcacgtcca ggttggaagt
     1381 aggctcatcc gcgatcagga gcgtggggtg gcaggccagg gcgatggcga tgacgatacg
     1441 ctgctgcatc cctccggaga gctcgtgcgg gtagcgcgcc gcgacgttct ccgggttcgg
     1501 gatcccgagg gaccgcagga gctccaccac gtgcccatgg accgtccgct ccagcctgct
     1561 gcggtagcgg cgaaggaaag gaacgcggcg cgccacttcc acgagagcgt ccgcgccgcc
     1621 ggtgcggtcc cgcatccggc tgagatggct cttgcgggcc tgagcttcgg gatcgtcccg
     1681 cccgtccagg ggatagagct cgttgtcgat ccgctgcagc cgcgcgcggt ggcggcggat
     1741 ggcggccagc tcccggcgga gcagcgcccg ctgcagcccg tgcccccgct tgccttccag
     1801 gccggccagg gtctcctcca gcatccgatc gttcaggtgg aaccgatagc tctccgccac
     1861 ctgctcgccg atggagagca ccgggttcag cgctgcggcg ggctcctgga agatcatgga
     1921 gatgtcgcag ccgcggatgg actccatgta ggcctcgctg cggtggacga tctcgatggc
     1981 cttcagccga tcctccgggt tcaggaacac gaagatgctt ccggagtcga tcttccccgg
     2041 ggcctgcacg atgcgcatca tggagcggac cgtgacgctc ttcccgcagc cgctctcccc
     2101 gaccagcccg tacgtctccc cgtggcggac cacgatggtt accccgttca gggcgcgtac
     2161 gactccctcg aaggtgtaga agttcgtgaa gaggtcctga gtcgttgcca gtgtatcctg
     2221 cgccacgcta tttccttctc accttgggat cgaagacgtc ccgcacggcg tcccccagga
     2281 ggttccaccc caggacgaag agcgagatga cgaaccctgg aatgaagacc gtataccagt
     2341 aagcgaaccg gttgcccggc gcgcccacga tccagttgcg gcccatggac acgatctggc
     2401 cccagtccgc cacgcccgta tccgagccca ggcccaggaa ggagagggcc gcggcggtca
     2461 ggatggtggc cccgatgttg agggaggcca ggatcaggac cgagtacatt gcgttgggaa
     2521 ggatgtgggt caggatcacg cggaggtgcg aggcgccgct ggcacgcgat gccaggatga
     2581 agtccttgtt tcgcgtgacc aggatgtcgc tccgcaggac acgggtgtag ctgggccact
     2641 ccaggatgat caggacgatg atgacggcct tcaaccctcg gccgatggcc accaccagcg
     2701 ccatcgccat gaccaggaac ggtatggcgt agaagatatc cgtaatccgc atgatgatct
     2761 cgtcgatcgc gccgccgaag taccccgcta tggcgccgag caggatcccg atcaggaggt
     2821 tggcggccac caccagcacg ccgatcttga aggcgctgcg cgtgccccag atcatgccgt
     2881 agagcatgtc gtactgctgc tgcgtggtcc ccagggggtg ctcggcgctg ggaggagagg
     2941 gctcgatctt ccagccgctg tgcggcatct tgaacgaatc ggggtacttc ggcggctcca
     3001 gcacgggagc cagcagggcg atcacgccga agaccaggat gagcgatagc ccgatgacgg
     3061 acagggggtt cttgatgaac cggcgcgcgg tgaagaggaa ctcgtccagc cgagggtgga
     3121 tcttgggagg ggaaacggat tggaccggag tacccatgag aagcctctct tcaggaaagt
     3181 cgaacccggg gatccacgta cgcatagatc agatcgacga tcaggttgat cacgacgtac
     3241 acggttccca ggaagagcac gttgaacatg atggccgcca cgtcgagctg gagcgcggcc
     3301 cgcgccgtcc accagccgat ccccttgcgg tcgaagatgg tctccgtgat gaccgcgccg
     3361 gacatgagac ccgcgaaggt gaggccggag accgtgatga cgggcagcat ggcgttgcgc
     3421 cgagcatgct tgtggtacag ggtgcggctg tccgccccct tggcccgggc ggtcatcacg
     3481 tagtcctggc ccagggactc cagcatgctg gagcgcatca gccgcatgat cagggcggag
     3541 ttgatgatga ccaggttcac cgtgggcagg atgaggcggt agaacgcgtc ccagaagatg
     3601 tcccagcgcc agttcaggat ggcgtctatg gtcaggaggt gggtgtactg gtggtagccg
     3661 ggcgcgttga ccacctcgtg ggcgctgttg gagagggacc ccggcgggaa gagcccggca
     3721 aacccgtaga ggatcatcat caggagcaga cccagccaga aggtgggcag cgagtagccg
     3781 atgatggcga agatgcgggt cccatggtcc aggggctcgt ccttgtgcac ggcgccgatg
     3841 gtgcccagga agattcccac caggatgatg aggggcgccg cgtagaggac cagctccagg
     3901 gtcgtgggga agaactgcat gaaccccgtc agcacggggg cgctcgcggt gatggagtag
     3961 ccgaactggc cgtgcagcag gttgccgatc caccggatgt actgcacggg cgccggatcg
     4021 ttcagcccgt acttggcgat catggcgtcc atcatttccg gggtgacccg gtcactggtc
     4081 acgtaggccg ccacgcgcat gccggggggc atcagcatca tgacggtgaa cacgagcagc
     4141 gacaccccga tgagcacgaa gatcatcagg aacagtcttc gaatgatgta ggatagcagg
     4201 ctcacgatgc agctccccgg tgagtgacga aggaaacgac ggggccgccg gcgcaccggc
     4261 ggccctcgaa acgatatccg gacaggatgc tacttcttgg tcagaacggg gaggagctcg
     4321 aactcctgac tctccatcgg gttgtagtag tagcccttca cccagtcctt catgaaccgc
     4381 cggcgcagcg gttgctgggc aagaatggct atggagtcct ggagccagat gtcctggagc
     4441 tgcgagtact cggcggcgcg cttggcgggg tccagctcga tggccgcctt ggcgaccagg
     4501 tcatccgccg ttggattctt gtagctctgc cggctggcga agaacccgtc cgaagacatg
     4561 tacggaccgg cgtaatcggc gggatccgga tagtccggtg cccaccccag gaagaacacc
     4621 gccatctgct tctgcttgtt catctcgttg tacacgggcc actccaccgc ccgggttccc
     4681 acctggaact tggggttgag cgacatcacg ttctccgcca ggatcttggc gcccgtctcg
     4741 cgctcggtgt tcccggtgtt gtatacgatg tcgaacttga atcccgtgtc ccacagcttg
     4801 ccgcccaggg ccttcttgaa gtagtccgcg gccttggtca tgtcgtgggg cttgctctcc
     4861 agcttgtcgt tcttgtaggg cagacccttg gggaacggcg tgaccggatc catg
//
LOCUS       DASRZS010000518         3571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2199765, whole genome shotgun sequence.
ACCESSION   DASRZS010000518 DASRZS010000000
VERSION     DASRZS010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2199765"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFH83_12665"
     CDS             <1..691
                     /locus_tag="VFH83_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487271.1"
                     /translation="MGLQARLMSQALRKLTATISKTRTCLVFINQIRMKIGVMFGNPE
                     TTTGGYALKFYASIRLEVRKVESIARGNEEVVGNKVRVNVVKNKVAPPFRKAEMDIMF
                     GKGISAVGSLLEAALQANIIEKAGSWYSYGKERIGQGVENARLFLESNPAVNAEVEKK
                     VRELLFPNRTTAGAAETKKEAPGSEPAREPAAQPGAVKTRPVPSQPGVTRPADPEDQD
                     PVDSDSRKELF"
     gene            710..1180
                     /gene="folK"
                     /locus_tag="VFH83_12670"
     CDS             710..1180
                     /gene="folK"
                     /locus_tag="VFH83_12670"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET6487272.1"
                     /translation="MGSNLGDRRGNLLEAWGRVAALGADALVSSLYETEPLYDTEQPL
                     FLNAVGSLSSEMHPQVMLALLHGIEVDLGRDRAAERRMGPRTLDLDILLCGDLLFEVE
                     NLTIPHPRIAERRFVLVPMLEIAPRLRDPRTGEAFAAQLDRLGSHGGVYSSRTQ"
     gene            1266..2108
                     /locus_tag="VFH83_12675"
     CDS             1266..2108
                     /locus_tag="VFH83_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET6487273.1"
                     /translation="MSDATTSEKQHFHLGDFDGPLDLLLFLIRKSEINIYDIPIASIT
                     EQYLAFLSYATRVDLDTMTDFHLMAATLLYIKSKMLLPLELTLDDELEDPRRELVEKL
                     IEYQRFRKLSDLMADREGEVEWMIERTRGQRILPFSDEGIWDQVSVWDLLQTFSSLLS
                     TLSSERIIDLYEEVSVNEKVTLIAELLEGQQEFLFTDLIRRRTILEIVCAFWAVLELV
                     KEGRIVVLQNRLFGDIRIRGRPGAPAPAGSADGGPAQASQDASNGVSSGAPPVEEASK
                     DGAE"
     gene            2095..2631
                     /gene="scpB"
                     /locus_tag="VFH83_12680"
     CDS             2095..2631
                     /gene="scpB"
                     /locus_tag="VFH83_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET6487274.1"
                     /translation="MELSKEAAVVEAVLLLENDPIEARRIASVTGLTTEAVGEAIAEL
                     RQALQLGGHGLEIVEIGGGFAFAPTKEHWERLRERYGKNAESRLSKAALETLAIVAYS
                     QPITRAEIESIRGVSADGMIKLLLSRSFIREVGRKDVPGKPVQYGTTKEFLKAFRLAT
                     IAELPKLDGLERDRFEQE"
     gene            2618..3448
                     /locus_tag="VFH83_12685"
     CDS             2618..3448
                     /locus_tag="VFH83_12685"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET6487275.1"
                     /translation="MSRNSDQEQEAGPEGEGIRLQVYLARCGLGSRRGCEELILKGRV
                     SVNGQPVTRLGARVLAKDAVTVNGRAVRPSAGGVYVALHKPRGFLCSNQDPAGRPMAS
                     SLFSSVIHERLHHVGRLDFLSSGLIFYTNDGEFTRLVSHPSSQIEKEYLVETGRPVEE
                     PFLRQYLKGIRVEDTVYRCKSYVLRGPRSVLITLTEGKNRELRKVFASRNIRVKRIHR
                     LRIGPVTLAGLQPGHFRMLNQRELRWFQSHGADRPGEPAPGARPPKRRTGGAPRGRKG
                     "
     gene            3445..>3571
                     /locus_tag="VFH83_12690"
     CDS             3445..>3571
                     /locus_tag="VFH83_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET6487276.1"
                     /translation="MIVAIDGPAGVGKSTIARRSAERSGFQYINSGSFYRAITLAA"
BASE COUNT          678 a         1108 c         1160 g          625 t
ORIGIN      
        1 catggggctc caggcccggc tcatgagcca ggcgctccga aagctcacgg ccaccatctc
       61 aaagactcga acctgcctcg ttttcatcaa ccagatccgc atgaagatcg gagtcatgtt
      121 cgggaacccg gagaccacca cgggtggata cgccctgaag ttctacgctt ctatccgcct
      181 ggaagtcagg aaggtggaga gcatcgcccg cggaaacgaa gaggtggtcg ggaacaaggt
      241 tcgcgtcaat gtcgtcaaga acaaggttgc cccgccgttc cgcaaggcgg aaatggacat
      301 catgttcggg aaggggatct cggcggtcgg cagtctcctg gaggctgcgc tccaggccaa
      361 tatcatcgaa aaggcgggaa gctggtactc ctacggcaag gagcgcatcg gccagggcgt
      421 ggagaacgcc cgcctgttcc tggaatccaa tcccgcggta aacgctgagg tagagaagaa
      481 ggtccgggag ttgctgttcc ccaaccggac cacggcagga gccgccgaaa ccaagaagga
      541 ggcgccaggt tccgagccgg ctcgagagcc ggccgcgcag cccggggccg tgaagaccag
      601 gcccgttcct tcccagccgg gcgtcaccag gccggcggac ccggaggacc aggaccccgt
      661 tgactcggac tctcgcaagg agcttttcta gctgatctac tacatcgctc tggggtcaaa
      721 cctcggagat agacgcggga acctgctgga agcatggggc cgggtagccg ccttgggggc
      781 agatgctctt gtgtcgtcgc tgtacgagac ggagcctctg tacgacacgg aacagccgct
      841 ctttctgaat gcggtcggct ctctttcctc cgagatgcat ccccaggtga tgctcgccct
      901 gctccatggg atcgaggtgg acctgggtag agacagggcc gccgagcgcc gcatggggcc
      961 gcgtacgctg gacctggaca tccttctgtg cggggacctc cttttcgagg tggagaacct
     1021 gaccatccct caccccagga tcgcggagcg ccgcttcgtg ctggttccga tgctggagat
     1081 cgcaccccgg ctccgcgatc cgaggaccgg agaggcgttc gccgcgcagc tcgaccggct
     1141 ggggagccac gggggtgttt actcttccag aacgcaatag tatacttctg tctcgatatc
     1201 agaaccttgc gtgccgtatc tcatcatccc catcctgctt gcgaagggag ccaagcctag
     1261 ccgcgatgtc tgacgcgacc acgagcgaga agcagcattt tcaccttggc gattttgatg
     1321 gaccgctgga cctgctgctc ttcctgatcc ggaagtccga gatcaacatc tacgacatac
     1381 cgatcgcgtc gatcacggag caatacctgg cgtttctgag ctacgccact cgggtcgacc
     1441 tggacaccat gacggacttt catctcatgg ccgctacgct gctctacatc aagagcaaga
     1501 tgctcttgcc cctggagctg accctggatg acgagctcga ggatcctcgg cgcgagctcg
     1561 tggagaagct gatcgaatac cagcgcttcc gaaagctctc ggacctcatg gcggaccgcg
     1621 aaggcgaggt cgagtggatg atcgagcgca cccgcgggca gcggatcctg ccgttttccg
     1681 acgaggggat ctgggaccag gtcagcgtct gggacctgct gcagaccttc tcctccctcc
     1741 tctccacgct gtcctccgag cggattattg acctctatga ggaagtgagc gtcaatgaga
     1801 aggtcacgct gatcgccgag ctgctggaag gacaacagga gttcctgttc accgacctga
     1861 tccggcgcag gaccatcctg gagatcgtct gcgcgttctg ggccgtgctg gagctggtaa
     1921 aggaagggcg catcgtggtc cttcagaacc ggctcttcgg tgacatccgg atccgaggcc
     1981 ggcctggagc gccggcaccg gcgggatctg cggacggcgg tccggcgcag gcgagccagg
     2041 acgcaagcaa cggcgtgtcc tcgggcgcgc cccctgtgga ggaagcgagc aaggatggag
     2101 ctgagtaagg aagcggccgt ggtggaggcg gtccttctgc ttgagaacga ccccatcgag
     2161 gcgcggagga ttgcctcggt caccggactc acgacggagg cagtggggga agctatcgca
     2221 gagcttcgcc aggctctgca gcttggcggc catggccttg agatcgtcga gatcgggggc
     2281 ggcttcgcct ttgctccgac caaggagcac tgggaacggc tgcgggaacg ctacggcaag
     2341 aacgccgaga gccgcctctc caaggctgcc ctggaaaccc tggccatcgt ggcatactcc
     2401 cagcccatca cgcgcgcgga gatcgaaagc atccgtggag tctccgcgga cgggatgatc
     2461 aagctcctcc tcagccgatc gttcatccgg gaggtgggca ggaaggacgt cccggggaag
     2521 cccgtacagt acgggaccac caaagagttc ctgaaggcgt tccgactggc caccatcgcc
     2581 gagcttccca agctcgatgg gctcgagagg gaccggtttg agcaggaata gcgaccagga
     2641 gcaggaggct ggaccggagg gagaggggat ccggctccag gtctaccttg cccgatgcgg
     2701 cctggggtct cgacggggtt gcgaagaact tatcctgaag ggacgtgtca gcgtgaatgg
     2761 gcagccggtc acgcggctgg gcgcccgagt cctcgccaaa gacgccgtga ccgtcaacgg
     2821 tcgagcggtc cggccttctg ccggcggggt ttacgtggca ctgcacaagc cgcgcgggtt
     2881 tctctgctcc aaccaggacc cggccgggcg gcctatggct agcagcctgt tctcttccgt
     2941 gatccatgag aggctccacc acgtcggaag actggatttc ctctccagcg gcctgatttt
     3001 ctacacgaac gatggcgagt tcacccggct cgtgtcccac ccaagctccc agatcgagaa
     3061 ggagtacctt gtagagaccg gtaggcctgt ggaagagccc ttcctccggc agtacctgaa
     3121 ggggatccgt gtcgaggata ccgtctatcg ctgcaagtcg tacgttctcc gtggaccccg
     3181 gtccgtcctc attaccctta ccgagggaaa gaaccgggag ctgcgcaagg tgttcgccag
     3241 ccgcaacatc cgggtgaagc gaatccaccg gcttcgtatt ggtccggtga ccctggccgg
     3301 cctgcagccc ggtcactttc gtatgctgaa ccagcgtgag ctgcgctggt tccagtctca
     3361 cggcgcggac cggccggggg agcctgcccc tggcgcgcgc ccacccaaac gccgcacggg
     3421 cggcgccccc cgggggcgca agggatgatc gtggcgatcg acggcccggc gggggtcggc
     3481 aagagcacca tcgcccggcg cagcgccgag cgctcgggtt ttcagtacat caactccggc
     3541 agcttttatc gcgccataac gctggccgcg c
//
LOCUS       DASRZS010000519         2515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3357041, whole genome shotgun sequence.
ACCESSION   DASRZS010000519 DASRZS010000000
VERSION     DASRZS010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3357041"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFH83_12695"
     CDS             complement(<1..654)
                     /locus_tag="VFH83_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487277.1"
                     /translation="MRISRIEPTVFLKREPGGLAQLLRITVDADAATPSARLRVLDRG
                     CASILPLGALPRGPSTHDVFLRERPAAREVVCTLLEEGRSSSAFAVTLQPVRHWTVHV
                     VQRSHHDVGYTDLPSRVLKQQARYLDDAIDLARETAAFPDASRFRIVIEAAWSLQGFL
                     EAAGLGRRRLMERLLRSGRFELAGLFGNLTTELCGHEEMIRALYPAFQLSRRYGFPVT
                     "
     gene            complement(667..>2515)
                     /locus_tag="VFH83_12700"
     CDS             complement(667..>2515)
                     /locus_tag="VFH83_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487278.1"
                     /translation="GLGRQASGMWAEMLYNRSFRPVSPARPIVWEWLRLDPEHYDARA
                     PFWHSGYEEMDWEPIGDPPFRTLGFEPFKGNDSLGIRNSTPGTEAGIRQRGVSLSRGS
                     GYRLRFLGGFAGRKTAAETREVRIRVTGESSPQRVIAEHTVGVSANQEEHGFELSTGD
                     FSGRAVVSFSFTWEGILHLSWASLTPVETVLGWRKDVVDLLRRVSPPILRFPGGCFAS
                     FYDWRDGIGPRSSRSPREPYFWGGLEENDIGIDEYLDLCAELGSEPQLCVNLMTGTPQ
                     AAADLVEYCNGPDDSRMGRLRRELGVTRKQTVRFWEMDNEAGRKWSALQYAAKVVEFS
                     RRMREVDPTITVMMEYYSWTPDWLPRMLELAGKHIDVVIHRDGDRTFMAKALATLTDY
                     NQRNGTSIRQANTEWLADFDSPEPFEDPEIPKTYDWEPSGNDYRKVLSYRQVRWFYAL
                     NAASRVLDYISYGGEFALANFNNCVNTWGGNIINASKEGAWLSPAGRVFELFRDFPGG
                     YPLVTTVEPLDRPELNVQACERSAPSGASAMGAERTVDLYAVNRGTQPISLSLRLPPG
                     IRPRRARVLDAETRLATNILGEDRIRYRETDGRALPELSPLSVTHLVLG"
BASE COUNT          436 a          820 c          848 g          411 t
ORIGIN      
        1 ggtcacgggg aacccatagc ggcgggaaag ctggaaagcc gggtagaggg cgcggatcat
       61 ctcctcatgg ccgcagagct cggtggtcag gttcccgaag aggccggcga gctcgaacct
      121 tccgcttcgc aggagccgct ccatgagccg ccggcggccc aagccggcgg cctccaggaa
      181 cccctggagg gaccacgcgg cttcgatgac gattcggaag cggctcgcgt cggggaaggc
      241 ggctgtctcg cgcgccaggt caatggcgtc gtccaggtag cgggcctgct gcttcagcac
      301 ccgagacggc agatccgtat aacccacgtc gtggtgcgac cgctgcacga cgtgcacggt
      361 ccagtgccgg accggctgga gggtgacggc gaacgccgag gaggagcggc cctcttccag
      421 aagcgtacag acgacctcgc gcgccgcggg gcgctcgcgc agaaacacat cgtgcgtcga
      481 aggcccccgg gggagcgcgc cgagcgggag gatggaggcg cagccgcggt ccaggaccct
      541 cagccgagca gagggagtcg ccgcatccgc atccacggta atgcgcagga gttgggccag
      601 accgccaggc tcgcgcttca ggaagaccgt gggttcgatc cggctgatcc gcatggcgcg
      661 ctcctcttaa cccaggacca ggtgcgtgac ggacaagggc gaaagctccg gcagagcgcg
      721 gccgtccgtc tcccgatacc ggatgcgatc ctcaccgagg atgttggttg ccaggcgcgt
      781 ctccgcgtcc agcacgcgcg cccgacgcgg ccggatccct ggcgggaggc ggagggagag
      841 ggagatgggc tgggttcccc gattgacggc gtacagatcc acggtccttt cggcccccat
      901 ggcactcgcg cccgatgggg cggatcgctc gcaagcctgg acgttcagct cgggccggtc
      961 taggggctcc acggttgtga ccagaggata gccgcccggg aaatcccgaa agagctcgaa
     1021 cacccttccc gctggtgaca gccacgctcc ttccttggac gcgttgatga tattcccacc
     1081 ccaggtgttc acgcaattgt tgaagttggc gagcgcgaac tccccgccgt aggagatgta
     1141 gtccagcact cgggaggctg cgttcagcgc atagaaccag cgcacctgcc ggtagctcag
     1201 caccttgcgg tagtcgttgc cggagggctc ccagtcatag gttttgggaa tctcggggtc
     1261 ctcgaacggc tcgggggaat cgaaatccgc cagccattcc gtattggcct gtcggatcga
     1321 agtcccgttc ctctggttgt aatcggtgag cgtggcgagc gccttggcca tgaaagtccg
     1381 gtctccgtcc cggtggatca cgacgtcgat gtgcttcccg gcgagctcga gcatccgcgg
     1441 aagccagtcc ggggtccagg agtagtattc catcatcacg gtgatcgtcg ggtccacctc
     1501 gcgcatgcgc ctggagaact ccaccacctt ggccgcatat tggagggcgg accacttgcg
     1561 tcccgcttcg ttgtccatct cccagaaacg gaccgtctgc ttgcgcgtca ctcccagctc
     1621 ccggcgcagc cggcccattc gggagtcatc cggaccgttg cagtactcga ccaggtccgc
     1681 ggccgcctgt ggggtaccgg tcatcaggtt cacgcagagc tgcggctcgc tgcccagctc
     1741 tgcgcagagg tcgaggtact cgtcgatccc gatgtcgttc tcctccagtc ctccccagaa
     1801 gtagggctct cgggggctgc gtgaggaccg cgggccgatg ccgtccctcc aatcgtagaa
     1861 gctggcgaag catccgccgg ggaaccggag aatcggaggg gaaacccggc gcaggagatc
     1921 caccacgtcc ttgcgccatc ccaggacggt ctctaccggc gtgagggagg cccaggagag
     1981 gtgcagaatc ccttcccagg tgaaggagaa ggagacaacg gcccggcccg agaagtctcc
     2041 ggtggagagc tcaaaaccat gctcttcctg gtttgcagag accccgacgg tgtgctcggc
     2101 aatcacgcgc tggggagaag actcgccagt gacccggatc cgaacctccc gagtctcggc
     2161 ggcggtcttg cgccccgcga acccaccgag gaaccgcaac cgatagccgc tgccgcggct
     2221 caggctgacg ccgcgttgtc ggatgccggc ctccgtgccg ggtgtcgagt tgcggatacc
     2281 gagggagtcg tttcccttga atggctcgaa ccccagggta cggaacggcg ggtccccgat
     2341 gggctcccag tccatctcct cgtagccgct gtgccagaaa ggcgctcggg cgtcgtagtg
     2401 ctcgggatcg agccgcagcc actcccagac gatgggtcgc gcgggtgata caggccggaa
     2461 gctccggttg tacagcatct cagcccacat gccggatgct tgtctgccca ggccc
//
LOCUS       DASRZS010000520         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5673087, whole genome shotgun sequence.
ACCESSION   DASRZS010000520 DASRZS010000000
VERSION     DASRZS010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5673087"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFH83_12705"
     CDS             complement(<1..542)
                     /locus_tag="VFH83_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6487279.1"
                     /translation="MTEPDRSASTLPAPGAGPLAPLGPSAAPLPASPGLPAPPPHDGA
                     APRVLIVDDVAENLKVVGSMLDGRGIDLCFATSGPQALASAHAAPPDLVLLDVAMPEM
                     DGYEVARRFKASEGLRSIPIIFITARTRTEDLVEGFRSGGVDYVTKPFVSAELIARVT
                     AHLELKRAQDVIAGQIGVLR"
     gene            complement(539..2749)
                     /locus_tag="VFH83_12710"
     CDS             complement(539..2749)
                     /locus_tag="VFH83_12710"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6487280.1"
                     /translation="MKPSGRLVVLVFVIAVAILIGAAGMTLRSTVDLIASDRLYVSKS
                     GLLMSIEELKSTVLGAVISERNFLLTGVDSDLRDYETAKVTTQVSISRIQGMAGQGGE
                     QRLVTELEQLLVSKFITLDEMIATRRAFGLGPQVRRILVDRSMELLDRIQDAADTLQN
                     MEKLRLQELERESAAKATVAQSISSMGDLIALLLLAFAFVILQREIARRSLAEASLVR
                     ARDMAEAANRAKSEFLANMSHEIRTPLNAILGFSELLAEGGASAERSQSHIEGIKAGG
                     KNLLLIIDDILDLSKIEAGRLDIRYAPVNLFAVVEEVERIFSASTRAKGLAFRIEISD
                     GIPRSVLIDEARLRQVLFNLVGNAIKFTSAGSITVRASSTTPQDGGAPGGGAGAARGS
                     TADGGGARAGESTVDLVIEVQDTGIGIPEDQRQAIFEPFNQGSRETLRFGGTGLGLTI
                     SRRLVDMMGGTLSVESQVGKGSTFRVRLGGVKVAAFQEEGRHEEPPAAELEFESQTVL
                     LVEDVESNRRVVREYLQPYRLTLAEAENGQEALELAGSLHPDLILMDMQLPVVDGFEA
                     IRRLKAAPATRRIPVIALTATATGEDVAEIRHLTEGYLRKPISRRELLQELSQFLTHH
                     TLAGADPGKRSAGGVGLGAFLARASADGTLRDGIGSAMREGILPAYHRLRVTLSVNDI
                     RAWCERLTALSGEQDLPELADYARELNASAGSFQIDRVIKALESFEPVTRIFEP"
     gene            complement(2865..>3109)
                     /locus_tag="VFH83_12715"
     CDS             complement(2865..>3109)
                     /locus_tag="VFH83_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487281.1"
                     /translation="GGKLYFLVIGKVRKVMDGEEVRRLVRVCGGSAPKAEGLQRLFRV
                     LKQERQDILMDAGIPSASSPVLEAIYTAIRGWKAGP"
BASE COUNT          500 a         1069 c         1024 g          516 t
ORIGIN      
        1 tccctcagga cccctatctg cccggcaatg acgtcctggg cccgcttcag ctccaggtgg
       61 gccgtcaccc gcgcgatcag ctcggcagag acgaacggtt tggtgacgta atccacgccg
      121 ccggagcgga acccttccac cagatcttcc gtccgggtcc gcgcggtgat gaagatgatc
      181 gggatggacc ggaggccctc cgaggctttg aaccgtcggg ccacctcgta tccgtccatc
      241 tcgggcatgg ccacgtccag gagtaccagg tccgggggcg ccgcgtgcgc gctggcaagg
      301 gcctggggac cgctggttgc gaagcagaga tcgatccccc gaccgtcgag catgctcccg
      361 accaccttca ggttttcggc tacgtcgtcc acgatcaaca cgcggggggc cgcgccgtcg
      421 tgcggtggcg gggcgggcag tccgggagag gcgggcagcg gggcggcaga cggccccagc
      481 ggggcgaggg ggcccgcccc cggcgcgggc agggtcgacg ccgaccgatc cggttccgtc
      541 atggctcgaa tatcctcgtc accggctcga aggattccag cgccttgatt acccgatcga
      601 tctggaagct gcccgcggaa gcgttcagct cccgggcgta atccgccagc tcggggaggt
      661 cttgctcccc ggacagcgcg gtgagccgct cgcaccaggc gcggatatcg ttgacggaga
      721 gggtaacgcg cagccggtga tacgccggca ggatcccttc ccgcatcgcc gaaccaatgc
      781 cgtcccgcag cgttccgtcc gccgacgcgc gggctagaaa ggcgcccaag ccgacccctc
      841 cggcggaacg cttccccggg tccgccccgg ccagcgtatg atgcgtaagg aactgtgaga
      901 gctcctggag gagctctcgc cgggagatgg gcttgcgaag gtagccctcc gtcaggtggc
      961 ggatctcagc aacgtcctcc cccgtagccg tcgccgtgag ggcgatcacc gggattctgc
     1021 gcgttgcggg ggcggccttc agccgtcgga tggcctcgaa cccgtccacg acgggaagct
     1081 gcatgtccat caggatcagg tccgggtgca gcgagcccgc gagctccagg gcctcctggc
     1141 cgttctccgc ctccgccaac gtcagccgat acggctgcag gtactcccgg accacgcgtc
     1201 ggttggactc gacgtcctcc accaggagga ccgtctggct ctcgaactcc agctccgcgg
     1261 cgggcggctc ctcatggcgg ccctcttcct ggaaggccgc caccttcacc cctccaagac
     1321 gcacgcggaa cgtggacccc ttgccgacct gactctccac ggagagggtg ccgcccatca
     1381 tgtccacgag cctccgactg atggtcagtc caaggccggt gccgccgaac cgcagggtct
     1441 cccggctgcc ctggttgaac ggctcgaaga tggcctgcct ctggtcctcc gggatcccga
     1501 tgccggtatc ctgaacttcg atcaccaggt ccacggtgct ttcaccggcc ctggcgcctc
     1561 cgccatcggc cgtcgagcct cgcgcggcgc cggcgcctcc gcccggggcg ccgccgtcct
     1621 ggggggtggt gctgctcgcg cgcaccgtga tggaccccgc cgacgtgaac ttgatggcgt
     1681 tgcccacgag gttgaacagg acctggcgga gccgagcctc gtcgatcagg acgctgcgcg
     1741 ggatcccgtc ggagatctcg atccggaacg ccagcccctt ggcgcgggtg gaggccgaga
     1801 agatccgctc cacctcttcc acgacggcga agagattcac cggagcgtag cggatgtcca
     1861 atcggcccgc ctcgatcttg gagaggtcca ggatatcgtc gatgatcagc aggaggttct
     1921 tgcctccggc cttgatgccc tcgatgtggc tctgggacct ctcggcggac gcgcccccct
     1981 ccgccagcaa ttccgagaac ccaaggatgg cgttcaacgg ggtgcggatc tcgtggctca
     2041 tgttggcaag gaactcgctc ttggcgcggt tggcggcctc cgccatgtcg cgcgctcgaa
     2101 ccaggctcgc ctcggccagg gagcgccggg cgatctcccg ctgcaggatg acgaacgcga
     2161 acgccaggag cagcagggca atgaggtccc ccatggagga gatgctctgc gccacggtgg
     2221 ccttggccgc ggactcccgc tccagctcct ggagcctgag cttctccatg ttctgaagcg
     2281 tgtccgcggc gtcctggatc cggtccagga gctccatgct gcgatccacc aggatccgcc
     2341 ggacctgcgg ccccagaccg aaggcgcgcc gggtcgcgat catctcatcg agggtgatga
     2401 acttgctcac caggagctgt tccagctcgg tcacgagcct ctgctcccca ccctgcccgg
     2461 ccatcccctg gatccgtgat atggagacct gcgtggtgac cttggccgtc tcgtaatccc
     2521 gcaggtcgga atcgacgccc gtcaggagga agttgcgctc ggagatcacc gcccccagga
     2581 ccgtggactt cagttcctcg atcgacatga gaagcccgct cttggagacg tagaggcggt
     2641 ccgaggcgat caggtccacc gtgctgcgca gagtcatccc cgcggcgccg atcaggatgg
     2701 cgacggcgat gacgaagacc agcacgacca gtcgaccaga aggcttcatg cgatccttgc
     2761 gggcagaagg atgcggtgcc ggcggaacgg gtgtcaagtt ggtatgatgc ccgccgggaa
     2821 gacgaaggga tggcgcgtct gcggtcgaaa ccgttatcga agggtcaggg cccggctttc
     2881 cagcctcgga tggccgtgta gatcgcctcc aggaccgggc tggaggcact cggtattccg
     2941 gcgtccatca ggatgtcctg ccgttcctgc ttgaggaccc gaaagagacg ttgaagtccc
     3001 tctgccttcg gcgcggagcc gccgcagact cggaccagac ggcgcacctc ctcaccgtcc
     3061 atcactttgc gaaccttgcc gatgaccagg aagtagagct ttccacctt
//
LOCUS       DASRZS010000521         7395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4518053, whole genome shotgun sequence.
ACCESSION   DASRZS010000521 DASRZS010000000
VERSION     DASRZS010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4518053"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFH83_12720"
     CDS             complement(<1..204)
                     /locus_tag="VFH83_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6487282.1"
                     /translation="MKSVVIVDDDEEFRESLAAVLREAGYDVTTTATPRDALQKAPKE
                     TPHVFLVDVDRASDGIAEFLGALR"
     gene            977..2911
                     /locus_tag="VFH83_12725"
     CDS             977..2911
                     /locus_tag="VFH83_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6487283.1"
                     /translation="MAAASAAERTAAERDASDAQNTIELATSRLVELKGAKGLGESYE
                     TVMCVGTDGVAFAASDPAYVGTSFSDRAYIKAALSGTPNAGEAVISKATGKPVVPVAA
                     PILSGGRVVGANVLLLDAQFLEDLVKGVVIGKTGYAYVIDRTGLIIAHPQVENVFKTN
                     LAELDGTREFTKKMIAGESGVSTYVFQGVPKTAGYAPVKATGWSAALTLPDSEYLAPV
                     NDVRNLILIVGAASLALGFVIFLAFSRSITKPLARAVAFAELVASGDLTQQLPIHQRD
                     EIGKLADALNGMSAKLKDMVATIQDSAEQVASSSEEITASAQKLAEGAQSQASTLEET
                     SASVEELTSSVEQVAEHSQDQAAAAEQGTASMAQVHKSIEDVSKNLSEIVGLASQSVD
                     NALQGAKAVSEVVEGMNTIQASGDRIGGIVTVISDIADQTNLLALNASIEAARAGEHG
                     RGFAVVADEVSKLADRSASSTKEIESLIKESTKNMTRGVETAKTSQAAMEQIRAASQT
                     VKEMIAGLSESMTQQVEAVKELSKALSSVSEMSQSISAATEEQTTNSKQVSKAVEAVN
                     EVTQSAASAAEEMSSATEQLASMAQELQRLMAQFKIDRGGSTGGNTPDRASAGENSHG
                     NGGNGNGKPQLALVAHRVPN"
     gene            3025..3387
                     /locus_tag="VFH83_12730"
     CDS             3025..3387
                     /locus_tag="VFH83_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015010220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6487284.1"
                     /translation="MGKRVLVVDDANFMRMIVKDTLTPRGFEICGEATNGAEAVTKYQ
                     QLKPDLVTMDITMKVKDGLQAAREILAADPNARIVMVTALGQEKTLLDSIALGVRDFV
                     VKPFSAERMLSAAAKALE"
     gene            3403..3924
                     /locus_tag="VFH83_12735"
     CDS             3403..3924
                     /locus_tag="VFH83_12735"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HET6487285.1"
                     /translation="MKINAKHVNPFVAAAMNVVNQITGIEVRRGHLSYKSKVEPSFSV
                     SIIIGIYGFLTGQVVYSLDGKLAQRLVDKLLEGKSPEEKRILFLDTLGEVANMITGNA
                     TALLNQKRDQTLNITTPAIAAGDNLSIHLVPKPALVLGLITQYGPIEISIAVEEKETL
                     TEMESFEDVLSET"
     gene            3942..6713
                     /locus_tag="VFH83_12740"
     CDS             3942..6713
                     /locus_tag="VFH83_12740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET6487286.1"
                     /translation="MEVKAEYRTIFCEEAADQLREWEESLLALEKDPADGEQLNRLFR
                     SIHTMKGSAGFIGYDALRKVAHDLESSLQQVREGSGSLDSATSSFLFEGHDLCRSMID
                     AFTEGREAPVDAAGFLSRLAALRSGTPSVPIGGSSATCRLSIRIDGPTREAYLRSFIV
                     KNRLERHGRILAVDPSPDSLKDSSGPFVYVVTLETAALPETIPGCISVDQVSVSLEAA
                     AEPTGAADPSAAAAGERGGKTARTEEVVRVSVQKLDTLLNLVGELVIQNSGFATTAQK
                     LREEHGKTPSVSDLEQKTEALSAITRDLQQGIMKARMLPVANVFERFHRVVRDLSKAS
                     GKPVALEIFGEETEIDKKVIDRISEPLVHLIRNAVDHGIEPAEERAARGKPTEGRVRL
                     AAYQDGDHICIEVSDDGRGLNRDSIVKKALEKGLIGPEEASMASAERILGFIFLPGFS
                     TAERISDISGRGVGMDAVKSAVQEMNGRLRVRSAQSGTTVTITLPLTMAIISALLVEV
                     ADSTFAIPLSAVREILKIRAEALKSAGGRAVIRLREEVLALVKLGGALETGQGSRQEA
                     ASNGTPVVVVDFEGRRIGLEVEKILGTREVVIKSLSRHYREIDGLIGASILGNGKIAL
                     IVDVETLISRHCHAADSGQRIGGKGIVDIEAIDYQSPQSARTFAPGPSGPPTAGQATA
                     VPTATPSPASEPLAASPPAEEPSGPETDLGALARKAINSEGTSLEEVNNASAIQASVS
                     LSQFMGREVRVSFPESRLIRVAEVAEAMGGEEATVGGIYIEVRGDLVGGILLVLPFAN
                     LLEMDDLLHGRPRRTARDLSSVDLSALCEMGNILAASFINTMADRTRLAVASGVPEIS
                     VDMCLPVIDSVLARFNQPGDEILVTQAILYGSGSETVVCNLMLFLEPASLQKLMDALE
                     AGVASGTAG"
     gene            6715..7200
                     /locus_tag="VFH83_12745"
     CDS             6715..7200
                     /locus_tag="VFH83_12745"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HET6487287.1"
                     /translation="MEGDVVNVRMAEMRMVESSRSLKTILGSCVGVILHDPQRRISAL
                     AHVMLPRRTRGDSSVGKYADTAVPALLDMLAGKGSRPSSLRAYLIGGARMFPVESDDI
                     GSIGDQNVAAARTALQEMAVPVVFEDTGGSRGRTVVFDNQSGEVSVRMLNPTGGGGGR
                     S"
     gene            7197..>7395
                     /locus_tag="VFH83_12750"
     CDS             7197..>7395
                     /locus_tag="VFH83_12750"
                     /inference="COORDINATES: protein motif:HMM:NF015650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HET6487288.1"
                     /translation="MNTALRSMALPILTDKTFNDFRALIFRKTGIHIRDGKQILISNR
                     LRRRLMALHLSSYEEYYCLLTS"
BASE COUNT         1498 a         2238 c         2427 g         1232 t
ORIGIN      
        1 gcgcagggcc cctaggaact ccgctatgcc atcgcttgcg cgatctacgt cgaccagaaa
       61 gacatggggc gtttcctttg gggccttctg gagagcgtcc cgcggcgtcg cggtggtcgt
      121 cacgtcgtag cccgcctcgc gaaggacggc cgccagactc tcgcggaatt cctcgtcgtc
      181 atcaacaatg actacgcttt tcatgttcgg ctcgtcggga gcgcacccgg agaggaaccg
      241 cgttcacgtc acccctcccc atagcggtcg tgcgtccttc cgtggagcgt tcagagaaac
      301 aactcctgag tttagtatag cagccaggcc ggggcaacac taccgctgcg cgatgcccct
      361 gggaatttcc gcactggcga ctggaacgga ctcgttcagc aatcctttgc atcacaggca
      421 attatcccac ctccagccct acctgtgggt ctcctggcgc gaaaatcccc tccggcgaaa
      481 ggtcacctaa ctccttagca gggagtgaat tatccgattc gttcggctgg cactcctttt
      541 gcagtaacag aggcatcggt acggagaaat gtctctatgt cgacgcgaaa cggttcgaac
      601 ccgtgacgaa aggaagtgga gaggaatgct gaagaacatc aagatcggga caaagctgat
      661 ggctgtagga acgctcataa cggtgacact gttggtggtc gtcgccgtca tagcactcac
      721 caaggcaacc cagggcctga ccgcctctga aaaccaacag ctcgccacgg gagcacggct
      781 ggtcgcagac caggttgacc gggtcttcca agaggagacc aagctagcac tggcgggtgc
      841 gcgggatccg agcattgttg ccgctgcgac cgtggcaaac gcgagaagcg tcccatcccc
      901 ggactcctcc gggaaaaccg cgtcaccgcc agccccagac agaagcggtt catcctccac
      961 aggttcagcc aagaccgtgg cagcggcgtc cgcggcagag aggacagcgg ctgagaggga
     1021 tgccagcgat gcgcagaaca cgatagagct ggcaacgagc aggctcgtcg agctgaaagg
     1081 agcaaaggga ctcggagagt cctacgaaac ggtgatgtgc gtcggcacgg atggcgtggc
     1141 ctttgcggcc tcagatcccg cgtatgtggg gaccagcttc agcgaccgag catacatcaa
     1201 ggccgccttg agcggaacgc cgaacgcggg cgaggcagtt atcagcaagg ccacaggaaa
     1261 gcccgttgtc ccggttgcag ctcccatcct ttccggcggc cgggtcgtgg gagcaaacgt
     1321 gctcctgctc gatgcgcaat tcctcgagga cctcgtcaaa ggagtggtga tcggaaagac
     1381 cgggtatgcc tatgtcattg accgcaccgg gctgatcatc gcgcatccgc aagtcgagaa
     1441 cgtgttcaag accaacctcg ccgagctcga cggtacccgc gagttcacca agaagatgat
     1501 cgccggcgaa agcggggtta gcacctacgt gttccagggc gtgcccaaaa cagcggggta
     1561 tgcaccggtg aaagcgaccg gatggagcgc ggcgttgacg ctcccggatt ccgaatacct
     1621 cgcacccgtc aacgatgtgc ggaacctgat cctgattgtg ggagctgcct cgctggcgct
     1681 ggggttcgtc atctttctgg cattctcccg ctccatcacc aagccgctgg caagagcagt
     1741 ggccttcgcc gaactggtgg cctcggggga tctcactcaa caactgccca ttcatcagag
     1801 ggacgaaatc ggcaagctcg ctgacgccct gaacgggatg tcggcgaagc tgaaggacat
     1861 ggtagccacg atccaggaca gcgccgagca ggtggcttcc tccagcgaag agatcaccgc
     1921 cagcgcccag aagctggccg agggcgcgca gagccaggct tcgactctgg aggagaccag
     1981 cgcatccgtg gaggagctga cctcttcggt ggagcaggtt gccgaacact ctcaggatca
     2041 ggccgcggcg gcagaacagg gcaccgcctc gatggcccag gtgcacaaat ccatagagga
     2101 cgtgtcgaag aacctgtcag agatcgtggg gctggccagt cagtcggtgg acaacgcgct
     2161 gcaaggggcg aaggccgtct cggaagtggt ggagggaatg aacaccatcc aggcaagcgg
     2221 ggacaggatc ggagggatcg taacggtcat ctccgacatt gccgaccaga cgaatctgct
     2281 cgcgctcaac gcctccatcg aggcggcgcg cgcgggagag cacgggcggg gattcgcggt
     2341 ggtcgccgat gaggtgagca agctggccga ccggagcgcg tcctccacca aggagatcga
     2401 gagccttatc aaggagagca cgaagaacat gacccgggga gtggagaccg ccaagacgtc
     2461 tcaggcagcc atggagcaga tacgcgcggc ctcgcagacc gtaaaggaga tgatcgcggg
     2521 gctctcggag tccatgacgc agcaggtaga ggcggtgaag gagttgtcga aggcgctgag
     2581 cagcgtgagc gagatgagcc agagcatatc ggcggcaacg gaggagcaga cgaccaactc
     2641 gaagcaggtg tcgaaggcgg tggaggccgt gaatgaggtg acgcagagcg ccgcctccgc
     2701 ggcggaggag atgtcctccg ctaccgagca gcttgccagc atggcgcagg agctgcaacg
     2761 cctgatggcg cagttcaaga tcgaccgggg gggctctacg ggcggcaaca cgccggaccg
     2821 ggcttcggcg ggagagaata gccacgggaa cggcggcaac gggaacggaa agccgcagct
     2881 tgcacttgtc gcacaccgtg ttcccaactg atcgtcggtg gcatcgagga gtctggaacg
     2941 gctgtcgcgg ccgtggggtc cccgccccca cgcgccgcac ttctgattcc cggatttgat
     3001 ccgctcgcta gaaggaagag aaaaatgggg aagagagtct tggtggtgga tgacgcgaac
     3061 ttcatgcgaa tgatcgtcaa ggacacgctg actcccaggg gttttgagat ctgcggtgag
     3121 gcgaccaatg gcgccgaggc ggtcacgaag taccagcagc tgaaacccga cctggtgacc
     3181 atggacatca ccatgaaggt caaggacgga ctacaggcgg cgcgcgagat cctggccgcc
     3241 gatccgaacg cgcgcatcgt gatggtgacg gccctcgggc aggaaaagac actcctggat
     3301 tccatagccc ttggggtaag ggatttcgtg gtgaagcctt tcagcgcgga gcggatgcta
     3361 tccgcggccg caaaggctct ggagtagaga ggaaggactc ccttgaaaat caacgccaag
     3421 cacgtcaacc ccttcgtagc ggcggccatg aacgtcgtga accagatcac cgggatcgag
     3481 gtgagacggg gccatctttc gtacaagtcc aaggtcgagc cgtccttcag cgtctcgatc
     3541 atcatcggga tctatggctt tctgaccggc caggtcgtct acagcctgga cggcaagctc
     3601 gcccaaaggc tcgtggacaa gctgctggag gggaagtctc cggaagagaa gaggatcctg
     3661 ttcctcgaca ccctgggtga ggttgcgaac atgatcaccg gcaacgcgac cgcgctcctc
     3721 aaccagaagc gcgaccagac cctgaacatc acgacaccgg ccattgccgc gggggacaac
     3781 ctcagcatcc atcttgtgcc gaagcccgcg ctcgtcctgg gtctgatcac gcagtacggg
     3841 cccatcgaga tcagcatcgc cgtcgaggag aaggaaaccc tcaccgagat ggagagcttc
     3901 gaagacgtgc tgagcgagac gtagcagcgg agggaatgcg catggaagtg aaggcggaat
     3961 accggacgat cttctgcgaa gaggccgccg atcagctccg ggagtgggag gaaagcctgc
     4021 tggcgctgga gaaagaccct gccgatggcg agcagctcaa ccgcctgttt cgctccatcc
     4081 acacgatgaa ggggtcggcg ggtttcattg gctatgatgc attgcggaag gtggcgcatg
     4141 acctggagtc ttccctccag caggtgcgag aaggcagcgg ctccctggat tccgccacct
     4201 cctccttcct cttcgagggt cacgacctct gccggtccat gatcgatgcg ttcacggaag
     4261 ggcgcgaagc gccagtggat gcggccggat ttctttcgcg cctggccgcg ctgcgttccg
     4321 gcacgccatc cgtccctatc gggggctcat ccgccacctg ccgactgtcg atccgtattg
     4381 acggaccgac ccgcgaagcg tatctgcggt cgttcatcgt caagaaccgg ttggagcgcc
     4441 acggaagaat cctcgccgtc gacccgtctc cggattctct caaagattcc agcgggccct
     4501 tcgtctacgt cgtaacgctc gagaccgccg cgctgcccga gaccattccc ggttgcatca
     4561 gcgtggacca ggtgagcgtc tccctggagg cagccgcgga accgacgggc gcggccgacc
     4621 cctcggccgc ggcggctggt gaaagggggg gcaagaccgc ccgtaccgag gaagtggtgc
     4681 gcgtgtccgt tcagaagctc gacacgcttc tcaacctcgt cggagagctc gtcatccaga
     4741 attccggctt cgcgaccacc gctcagaagc tgcgggagga acatggcaag accccctccg
     4801 tttccgatct cgagcagaag acggaggcgc tctccgccat cactcgggac ctgcagcagg
     4861 gcatcatgaa agcccggatg cttcccgtgg cgaacgtgtt cgagcggttc catcgcgtgg
     4921 tgcgcgatct ctcgaaggcg agcggcaagc cggtcgcgct ggagatcttc ggagaagaaa
     4981 ccgagatcga caagaaggtg atcgaccgga tcagcgaacc cctggtgcat ctcatccgca
     5041 acgcggtgga tcacggcatc gagccagcgg aggagcgcgc cgcaagaggc aagcctacgg
     5101 agggaagggt acgcctggcc gcgtaccagg acggggacca catctgcata gaagtgtcgg
     5161 acgacgggcg tgggctgaac cgggattcga tcgtgaagaa ggcgctggag aaaggcttga
     5221 tcgggcccga ggaagcctcc atggctagcg cggagcggat cctgggattc atattccttc
     5281 ccgggttcag cacggcggag cgcatatcgg acatctccgg acgcggggtg gggatggacg
     5341 ccgtgaagtc cgcggtgcag gaaatgaacg gccgcctgcg ggtgcgctcc gcgcaatccg
     5401 gcaccaccgt gaccatcacg ctgcccctga ccatggcgat catctccgcg ttgctggtgg
     5461 aagtcgccga ctcgaccttc gcgatccccc tttccgcagt gagagagatc ctgaagatcc
     5521 gcgcggaggc gctgaagagc gcgggcgggc gggcggtcat ccgcctgagg gaagaggttc
     5581 tggcactggt caagctgggt ggcgcgctcg agacggggca gggctcgcgg caggaagcgg
     5641 cgagcaacgg caccccagtg gtcgtggtgg atttcgaggg caggaggatc ggcctggaag
     5701 tggagaagat cctggggacc cgggaggtcg tcatcaagag cctgagccgg cactaccggg
     5761 agatcgacgg cctgattggc gcctccatcc tgggaaatgg gaagatcgcc ctgatcgtgg
     5821 acgttgagac gctaatcagc cgccactgcc atgcggccga ctccggccag agaatcggcg
     5881 gaaagggaat cgtcgacatc gaggctatcg attaccagtc tccccagtcc gcccggacct
     5941 tcgcaccggg accctcggga ccgcccacgg cggggcaggc caccgctgtc ccgaccgcta
     6001 cgccttctcc cgcgtcagag cccttggcag cctcgcctcc tgccgaggag ccttccggcc
     6061 cagagacgga cctgggcgcg ttggcgcgga aagccatcaa cagcgaaggc acttctctgg
     6121 aggaggtgaa caacgccagc gccatccagg cctcggtgtc cctctctcag ttcatgggcc
     6181 gcgaggtccg ggtaagcttc cccgagtcgc gcctcatacg ggtcgccgag gttgcggagg
     6241 ccatgggggg agaagaggca accgtcggcg ggatttacat cgaggtgcgg ggcgacctgg
     6301 tgggtggaat tcttctggtt ctccccttcg ccaacctgct tgagatggac gacctccttc
     6361 acggacgccc caggcggacc gctcgtgacc tttccagcgt cgatctctcg gcgctgtgcg
     6421 agatgggaaa catcctcgcc gcgagcttca tcaataccat ggccgaccgc acccgactgg
     6481 ccgtggcctc cggggtcccc gagatcagtg tcgatatgtg cctcccggtg atcgactcgg
     6541 tcctggctcg attcaatcag cccggcgatg agatcctggt gacgcaggcc atcctctacg
     6601 gctctgggtc ggaaacggta gtctgtaacc tgatgctctt cctggagccg gcttccctgc
     6661 agaagctcat ggacgccctc gaagcggggg tcgcctcggg aacggctggc tgacatggaa
     6721 ggggacgtcg tgaacgtgcg gatggcggag atgcgcatgg tggagagctc ccgcagcctc
     6781 aagaccattc tcggttcgtg cgttggcgtg attcttcatg acccgcagcg gcggatcagc
     6841 gccctcgccc atgtgatgct tccccgcaga acccgcgggg actcctccgt tggcaagtac
     6901 gccgacacgg cggtccccgc tcttcttgac atgctggcgg gcaagggaag ccgcccctct
     6961 tcgctgcgcg cttaccttat cgggggcgcc cggatgtttc cggtggagtc ggacgatatt
     7021 ggctcgatcg gggaccagaa cgtcgcggcc gctcgcacgg cgctccagga gatggccgtt
     7081 cccgtcgtct tcgaggatac cggcgggagc cggggcagga cggtggtctt cgacaaccag
     7141 agcggcgagg tctcggtcag gatgctgaat cccacgggtg gcgggggagg caggtcgtga
     7201 acaccgcgct gcggtccatg gcgcttccca tcctgactga caagaccttc aacgatttcc
     7261 gggcgctgat cttcaggaaa accgggatcc atatacgaga cgggaagcag atcctcatct
     7321 ccaaccggct gcggcgacgc ctcatggctc tccatctctc ctcgtacgag gaatactact
     7381 gcctgctcac ctccg
//
LOCUS       DASRZS010000522         3669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3755173, whole genome shotgun sequence.
ACCESSION   DASRZS010000522 DASRZS010000000
VERSION     DASRZS010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3755173"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VFH83_12755"
     CDS             <1..939
                     /locus_tag="VFH83_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487289.1"
                     /translation="LSGSLQYQKRSQTAYQARRSLEGHAESVVAALASDPTPQSDSPL
                     DPVWSLVGTSEDGTRLSLSDVSSALNPNWVQKNVFSKTSLKSTLASEGAADVLQQRRE
                     DRGLSEGIGDAYGDLFVEGALGRYFTAYGYANINVTDEFALQKLYAVRTGDSAAAEVF
                     HSQVQRLLMDQRILKPDELRTFLGSAYDALYPVMSAEPCMNVHFVDPDVLTALLAYPE
                     LKVPHPAQAAQQILETRGRTELSQEDLVRIIGAPSTSRIYQYLGVVTWFWRISASSGA
                     SRVDLVVARVPESAEGPARNASPPRYATVEERLGSP"
     gene            936..2009
                     /locus_tag="VFH83_12760"
     CDS             936..2009
                     /locus_tag="VFH83_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487290.1"
                     /translation="MILGYGEEHGGEVTVHGPQGWDALLMSRLDFDVRVFDVPPVSDR
                     DLEGLVRLRLRSLYPASPQETAFDYQLVRRGARRQAVVFVSRRRTVDAYRRAAGKRPL
                     VLPYQLLAPRVPERGDWRAWIGNLDWVELLVYRDGALLSSTARRPPSEPQEGTDPATG
                     FDLVAASQDLGLEGRVNGSTFVALEALGSKRRLAGLFPPKRRRALLPWRGRIALLTAA
                     VIVLSLLVFYRYVRQVEDYGSRSSARAAGLERSNQDALAIQRDLETLRAEKARLDARI
                     PRDLYLLLSELSTGLGDAARIQGLTVRDDGFQVDAVGTNPLALMEGLKARPDFSDLLL
                     SQVVPDPASGKERFSISGVFRGR"
     gene            1999..2880
                     /locus_tag="VFH83_12765"
     CDS             1999..2880
                     /locus_tag="VFH83_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487291.1"
                     /translation="MVDKRTVTLLWLLGTTVAVVATAMGVSLGLERLRDARQTAARAE
                     ERIARIQGSLPSTTELLAARAELRTQIDSESGRFYRAGEMNPYLFGTIVKQKLSALGL
                     AAKRYQVVEIKGTSYVEYSVTGSARGFIAFLRDVSTSSRLWTIPSMSVTLRQGSDVVE
                     AVVRIGYALVDKPRTASPAAAWARAGALAPEAILPLFVGKKTAKPAPAAVASPQPAPA
                     SQPAPKVPVAAPWLRYMGRSTGPDGSASVYVKDTKSGKVVRAALGVTVGGWTLVSEDD
                     AGLVLTFGEDLYTVSKR"
     gene            2883..3587
                     /locus_tag="VFH83_12770"
     CDS             2883..3587
                     /locus_tag="VFH83_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6487292.1"
                     /translation="MRVWTPGLLSVSLGAVLLVASCATQKGADPMAFDKAPLFGMIYD
                     ADNQPCAGVQLAVDGADGPMSDIRGRFVVPDLARGQHSMEARKDGFERLAVTVTFLNK
                     TDVLHLQMTSFDQLLAMARAAFTDKRWGDGEGYLRRAENLNPTDSVLRYLFAVDAYRT
                     GAFSTAVDYLDLITESDGQKPAVLLLLADIYEKGLADLRKAIANLEAYLKLRDDPEVQ
                     KRLDALKAKVQAGPGG"
BASE COUNT          599 a         1175 c         1240 g          655 t
ORIGIN      
        1 ctcagcggat cgcttcagta ccagaagagg agccagaccg cctaccaggc gcgtcgctcc
       61 ctggagggcc atgcggagag cgtggtggct gccctggcct cggatcccac gccgcagtcc
      121 gattcaccac tggatcctgt ctggagcctc gtaggcacgt cggaggatgg cacccggctc
      181 tcgctctccg atgtgtccag cgctctgaac cccaactggg tccagaaaaa cgtgttctcc
      241 aagacgtcgc tgaagtccac actggcctcg gaaggcgccg cggatgtgct tcagcagcgc
      301 cgggaggacc ggggtctttc agaaggcata ggggacgcct acggagacct cttcgtggaa
      361 ggagccttgg ggagatactt caccgcctac ggctatgcga acatcaacgt aacggacgag
      421 ttcgcattgc agaagctgta tgccgttcga accggcgact ccgcggcagc agaggtcttc
      481 cactcccagg tccagcgctt gctgatggac cagcgcatcc tgaagccgga cgaattacgg
      541 acgtttctgg gatcggcgta tgatgcgctg tacccggtca tgagcgcaga gccatgcatg
      601 aatgtccact tcgtggatcc ggacgtcctc accgctctcc ttgcctaccc ggagctgaag
      661 gtcccgcatc cggcgcaggc cgcgcaacag atccttgaga cccggggtcg gacggagctt
      721 tcccaggagg atctggtcag gatcatcgga gcgccctcta ccagccggat ctaccagtac
      781 ctcggcgtgg tcacctggtt ctggcggatc tccgcctcct cgggagcctc tcgcgtcgac
      841 ctggtggtgg cgagggtgcc ggagtccgcc gagggccccg cgcgcaacgc aagccctccc
      901 cgctacgcca ccgtggaaga gaggctgggc tctccatgat cctgggctac ggcgaagagc
      961 acggcgggga ggttacggtc cacggcccgc aggggtggga cgcgcttctc atgtcccggc
     1021 tcgatttcga cgtgcgggtc ttcgacgtgc cgccggtgtc ggatcgcgac ctggaaggac
     1081 tggtccgtct gaggctgcgc agtctctacc ccgccagccc ccaggagacc gcgttcgact
     1141 atcaactggt gcggcgtgga gcgcggcggc aggccgtcgt gttcgtctcc cggcgaagga
     1201 ccgtggatgc ctaccgccgg gccgcgggca agagaccgct ggtgctcccg taccagctcc
     1261 tggcgccccg cgtgccggag cggggcgact ggcgagcgtg gatcggcaat ctcgactggg
     1321 tggagcttct cgtctatcga gacggcgcgc tcctttcgtc cacggcgcgg cggccgccct
     1381 ccgagccaca ggaggggacg gacccggcta ccggattcga tcttgtggct gcttcccagg
     1441 atctgggact ggaagggcgt gtcaacgggt ccaccttcgt ggcgctcgaa gcgctcggat
     1501 cgaagcggcg gctggctggc ctctttccgc cgaagcgccg cagggcgctc ctcccgtggc
     1561 gcggacgcat cgccctgctg accgctgcgg tcatcgtcct gagcctcctt gtcttctata
     1621 ggtacgtccg tcaggtcgag gactacggct cgcgctcgag cgctcgcgcg gcgggtctgg
     1681 agagaagcaa ccaggatgcc cttgccatac agcgggatct ggagacactc cgcgcggaga
     1741 aggcgcgcct cgatgcgaga atcccgcggg atctgtacct gctcctgtcc gagctgtcga
     1801 ccggcctggg ggacgctgcc cggatccagg gccttacggt ccgggacgac gggtttcagg
     1861 tcgacgccgt gggaaccaat ccgctggccc tcatggaagg gctgaaagcc cggcctgact
     1921 tctccgacct gcttctctct caagttgtcc cggatcccgc gtcgggtaag gagcgttttt
     1981 cgatctcggg ggtgttccgt ggtcgataag agaaccgtga cgctcctgtg gctcctggga
     2041 acgaccgttg cggtcgtcgc gacagccatg ggggtgagcc tgggtctgga gcgactgaga
     2101 gacgcgcggc agacggcggc ccgggcggaa gagcgcatcg cgcggatcca ggggtccctc
     2161 ccttcgacca cggagctgtt ggcagctcgc gcagagcttc ggactcagat cgattccgaa
     2221 tcggggcgat tctaccgcgc cggcgagatg aacccctacc tcttcgggac catcgtcaag
     2281 cagaagcttt ctgccctggg tcttgccgca aagcgctacc aggtggtgga gatcaagggc
     2341 acgagctacg tcgagtattc agtgaccgga agcgcccgcg gcttcatcgc cttcctgcgg
     2401 gacgtctcga catcctcgag gctctggacc attccgtcga tgagcgtgac gctccggcag
     2461 ggctcggatg tcgtcgaggc cgtcgtgagg atcggatacg ctttggtgga caagccccga
     2521 accgcgtcgc ccgcggcggc gtgggcgcgg gcaggcgcgc ttgcgcccga agccatcctg
     2581 ccgctcttcg ttgggaaaaa gaccgcgaag ccagctccgg cggccgtcgc ttcgccacaa
     2641 cccgcgcccg cgtcgcagcc cgcccccaag gtgccggtcg ccgcgccgtg gctgcgctac
     2701 atgggacggt ccaccgggcc ggacgggagc gcctcggtct acgtcaagga cacgaagtcc
     2761 gggaaggtgg ttcgtgcggc gcttggcgtg acggtcggcg gctggaccct ggtgtccgag
     2821 gatgacgccg ggctggttct caccttcgga gaggatctct acacggtgag caagaggtaa
     2881 cgatgagagt ctggactccg ggcctccttt ccgtctccct gggagcggtg ctcctcgtag
     2941 cctcgtgtgc aacgcagaag ggagcggacc ccatggcgtt cgacaaggct ccgctcttcg
     3001 ggatgatcta tgacgcggac aaccagccct gcgccggagt ccagctcgcg gtggatgggg
     3061 ccgacgggcc aatgagcgac atccgcgggc ggtttgtagt gccagacctg gctcgcgggc
     3121 agcactccat ggaagctcgc aaggacgggt tcgagcgcct cgccgtcacg gtcacgttcc
     3181 tgaacaagac ggacgtcctg catctgcaga tgacctcgtt cgaccagctt cttgcgatgg
     3241 cccgggccgc gttcactgac aagcgatggg gtgacggtga agggtacctg cgtcgcgccg
     3301 agaacctgaa ccccacggac tcggtcctgc gctacctctt tgccgtcgat gcctatcgga
     3361 ccggagcgtt ctccaccgcg gtggactacc tggacctgat cacggagtcc gacgggcaga
     3421 agccggcggt gctcctcttg ctggccgaca tctacgagaa gggtcttgcg gatctgcgga
     3481 aagccattgc gaacctggag gcgtacttga agcttcggga cgaccccgaa gtgcagaagc
     3541 ggctggacgc gctgaaggcc aaggtgcagg cggggccagg agggtagctt cccttcgccg
     3601 gcttctctga cccgatagcg accgcgcgtc gttctgagga gaatgtatgg cttcaaaacg
     3661 tgtgttctt
//
LOCUS       DASRZS010000523         3840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5675482, whole genome shotgun sequence.
ACCESSION   DASRZS010000523 DASRZS010000000
VERSION     DASRZS010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5675482"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1379)
                     /locus_tag="VFH83_12775"
     CDS             complement(171..1379)
                     /locus_tag="VFH83_12775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HET6487293.1"
                     /translation="MPARRFLADGHLIDSGLLTGMLNLIGDAGADYQVVSFDMGKVRT
                     DPSHIELEVRCRDEAQLASLTGLLVDQGCYEKEVQEAVVRPAPGDSCVPDDFYATTNR
                     RTQVFHHGRWVDVADMRMDGVIVEKAGRLACRLIRDVKAGEGVVCTSRSVRIFPLFSE
                     QDGEEFGFMGSDVSSERSVDVAIGRVAEELRRRKAAGGRVIVVAGPVVLHTGGGPALA
                     ALVRGGYVSGLLAGNALGVHDVESALFGTSLGISLLTGRPVFEGHRNHIRAINTIFSH
                     GSISAAVEAGAVRSGVMFECVKAGVPFVLAGSLRDDGPLPETVMDMLAAQRAYAQMVK
                     GADVVIMLSSMLHSIATGNMIPASARTICVDINPAVVTKLSDRGSGQTIGIITDVGLF
                     LRALVEKLQR"
     gene            complement(1385..1933)
                     /locus_tag="VFH83_12780"
     CDS             complement(1385..1933)
                     /locus_tag="VFH83_12780"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6487294.1"
                     /translation="MRKLIQQYAKDLTSGRLGSGYEHSFRVYHLTREIGEGIEYDDDI
                     LHAACFLHDVEMSVGHPKSSADRAEAILRETGFDPDKIPLVTDAILNHMPEGNPQRVE
                     AKLLFDANLLDSIGAVGFARLSVGAFIWYNMKTMQEIHKYIARELSYTERFHFDKSRE
                     LAREKIAFTRAAIAQFAKELEL"
     gene            2216..2698
                     /locus_tag="VFH83_12785"
     CDS             2216..2698
                     /locus_tag="VFH83_12785"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET6487295.1"
                     /translation="MIDDKDRKLLDLLQENARLSNAELAEAVGLTASSVHERVKKLER
                     QGIIKGYVAVLDAEKLGKPLVAFVRLTVQTHPQDGQKNGLLELCKSEPDIMECHNVAG
                     EDCFVLKVRAEGPKKLERLLSAIRGSTETNRSVTNIVLSTYKESARVSPAPAHTEDLT
                     "
     gene            2695..2970
                     /locus_tag="VFH83_12790"
     CDS             2695..2970
                     /locus_tag="VFH83_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487296.1"
                     /translation="MSNAELALVMNGLGRTAPGKVPVLRVVLLSGEALRIPRLSSHLR
                     VLTGTAWVSQGGKDRIIEAGQCYKVPAGRDRPIVSGLRGEPLLVEMR"
     gene            complement(3047..>3840)
                     /locus_tag="VFH83_12795"
     CDS             complement(3047..>3840)
                     /locus_tag="VFH83_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET6487297.1"
                     /translation="LAFILSRIGAVSREFSLKIVYVSIFLYLGSGVIGTFHHLYWTGT
                     PTPIIALGAVFSALEVVPLALLGFETAQNLRTLKQGGANYAYRWPLYFFIAVAFWNVL
                     GAGVFGFLINPPIVLYYAQGMDTTPLHAHTALFGVYGFFAIALMLFSVRHIVAIRAWS
                     DRMLKWGFWLLNGGLMGMTVLNLVPAGFYQLYHAITYGVWYARSPEITSGPVIKFLNY
                     LRIVPDTVFGIGGVVIFLFVVRATWMSFARRRQSASGGSKTNSAS"
BASE COUNT          725 a         1201 c         1234 g          680 t
ORIGIN      
        1 agccctttgc ggacagccag ctcagggcct ggcttgcctg gccggtgttg taccccaggt
       61 catcggcaag ccgcgcgtgg ctgagggtat cgcccttgcc atagcggagg agaaccttga
      121 cttcgagcgg gtgaagctcc cgggaagggg cggggtggtc cattgacgtg ttacctctgg
      181 agtttttcca cgagcgctct caggaacaac cccacatcgg tgatgatgcc aatggtctgt
      241 ccggagcctc tgtcggagag ttttgtcacg acggcggggt tgatgtcaac gcagatggtg
      301 cgggccgagg cggggatcat gttgcccgtg gctatggagt gcagcatgct cgacagcatg
      361 atgaccacgt ccgcgccctt caccatctgc gcataagcgc gctgcgctgc cagcatgtcc
      421 atgacggttt ccggcagcgg gccgtcatcg cgcagcgagc cggcaagaac gaaaggcacg
      481 ccggctttca cgcactcgaa cattacacct gagcgcaccg ctcccgcctc taccgcggca
      541 ctgatcgagc cgtgggaaaa gatcgtgttg attgcgcgga tgtggttgcg gtgtccctcg
      601 aagaccggcc gccccgtcag gagggagatg cccagggacg tgccgaacag ggcgctttcc
      661 acgtcgtgca cccccagcgc gttgcccgcg aggagacccg acacgtaccc gcccctgacc
      721 agcgcggcaa gggcgggccc gccgccggtg tgcaggacca ccggtccggc cacgacgatg
      781 acccgtccac ccgctgcttt tcgccggcgc agctcttcgg ccacgcgacc gattgccacg
      841 tccacgctcc tctccgagga gacgtcgctg cccatgaaac cgaactcttc accgtcctgc
      901 tcggagaaca ggggaaagat gcgcacggaa cggctggtgc acaccacccc ttcaccggcc
      961 ttcacatctc ggatgagacg gcaggcaagg cggcccgcct tctccacgat gacaccgtcc
     1021 atccgcatgt cggccacgtc cacccaccgc ccgtggtgga aaacctgcgt gcgccggttg
     1081 gtcgtggcat agaaatcgtc gggcacgcag gagtcaccgg gcgcgggccg cacgacggcc
     1141 tcctgcactt ctttctcgta gcagccctgg tccaccaaca ggcccgtcag ggaggcgagc
     1201 tgcgcctcgt cgcggcatcg cacctcgagc tcgatgtggg acgggtcggt gcgcaccttt
     1261 cccatgtcga aggagaccac ctggtagtcc gctcccgcgt cgccgatcag gttcagcatc
     1321 ccggtgagga ggccggaatc gatgaggtgg ccgtcagcca ggaaccggcg cgcgggcacg
     1381 gctcctacag ctccagctct ttggcgaact gggcgatggc ggcgcgcgtg aaggcgatct
     1441 tctcccgcgc cagctcccgg ctcttgtcga agtgaaaccg ctccgtgtag gagagctccc
     1501 gggcgatata cttgtggatc tcctgcatcg tcttcatgtt gtaccaaatg aaggctccca
     1561 ccgacaggcg cgcgaagccc accgcgccga tggaatccag caggttggcg tcgaacagca
     1621 gtttggcttc gaccctctgg gggttgcctt ccggcatgtg gttcaggata gcatccgtca
     1681 ccaaggggat cttgtcgggg tcgaaccccg tctcccgcag gatggcctcc gcgcggtccg
     1741 cggagctctt gggatggccg acggacatct ccacgtcatg gaggaagcag gccgcatgga
     1801 ggatgtcgtc gtcgtattcg atcccttcgc cgatctctcg cgtcaggtga tacactcgga
     1861 aggaatgctc gtagccggac ccgagcctcc ccgaggtcag gtccttggca tactgctgga
     1921 tgagttttcg catgggcgaa gtctaccgaa gagcatctgc cgatgcaacg aggcgggtgc
     1981 ggagcgtctg tttctgggcc tccggccccg cctgactccc tgggcagttt ttccggcgcc
     2041 cacctcgcca ccctcgggaa aatgcgcgaa gcgcagggaa aaccagcgct ttctgcgttt
     2101 tccacaggtt cacgctctgg ccccgccgga tttgccttcc gaaccaaatt cggagtttat
     2161 accctagttc cgaatatgag ttgacacttg ccttgaatgg caatataata ttcgtgtgat
     2221 cgacgacaag gaccgcaaac tgctggatct cttgcaggaa aacgccaggc tttccaacgc
     2281 cgagcttgcc gaggcggtgg gtctgacggc ttcctcggtc cacgagcggg tcaagaagct
     2341 cgagcgccag gggatcatca agggatacgt cgcggtgctg gacgcagaga agctcggtaa
     2401 gccgctggtg gctttcgtgc gtctcacggt ccagacccat cctcaggacg gtcagaagaa
     2461 cggcctcctg gagctttgca agtccgagcc ggacatcatg gaatgtcaca acgttgccgg
     2521 tgaggactgc ttcgtcctga aggtccgggc agaaggaccg aagaagctgg agcggctcct
     2581 gtcggccatc cgtggaagca ccgaaaccaa tcgttcagtg acgaatatcg ttctgtccac
     2641 ctacaaagaa tcggctcggg tgtcccccgc gcctgcgcat acggaggatc tgacatgagc
     2701 aacgctgagc ttgcgctggt tatgaacggc ctggggcgga cggcgcccgg caaggtgccg
     2761 gttctgcgcg tggtcctgct gtccggtgag gctctacgga tcccgcggct cagctcccac
     2821 ctgcgggtcc tgacgggtac cgcgtgggtc agccagggcg ggaaggatcg gatcatcgag
     2881 gcggggcagt gctacaaggt ccctgctgga agagatcgcc ccatcgtctc gggtctgcga
     2941 ggtgagcccc tcctggtgga gatgcgctga ggccggggcc aagcggcctc tcgcgtcggg
     3001 aaacggtccg gccgagactt cccggccgaa ccgttccagc ggaagctcag gacgcgctgt
     3061 tcgttttgga gccgccgctc gccgattgcc gacgcctggc aaacgacatc cacgttgccc
     3121 tgacgacgaa caggaagatc acgacaccgc cgatcccgaa gaccgtatcc ggcactatcc
     3181 gcaggtagtt caggaacttg atgaccggtc ccgatgtgat ctcggggctg cgggcgtacc
     3241 agacgccgta cgtgatggcg tggtagagct ggtagaatcc cgccggcacc agattgagga
     3301 cggtcatacc catcagcccg ccgttgagaa gccagaagcc ccacttgagc atccgatcgg
     3361 accacgcgcg gatggcgacg atgtgccgca cggagaagag catcagagcg atggcgaaga
     3421 acccgtagac tccgaagagg gccgtgtgag cgtgcagcgg ggtggtgtcc atgccctgcg
     3481 cgtagtagag aacgatcggc ggattgatga ggaagccgaa cacgcccgca cccagcacgt
     3541 tccagaacgc gacggctatg aagaagtaca gaggccagcg gtaggcatag ttggcgccac
     3601 cctgcttcag cgtgcgcagg ttttgggcgg tctcgaaccc caggagggcc aggggaacga
     3661 cctccagcgc cgagaacacg gcccccagcg ctatgatggg agtcggggtt ccggtccagt
     3721 agagatgatg gaacgtgccg atcacgccgc ttcccaggta gaggaagatg ctcacgtaga
     3781 cgatcttgag actgaactcc cgactcaccg ctccgatgcg ggaaaggatg aaggccaaca
//
LOCUS       DASRZS010000524         3368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2988639, whole genome shotgun sequence.
ACCESSION   DASRZS010000524 DASRZS010000000
VERSION     DASRZS010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2988639"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFH83_12800"
     CDS             <1..669
                     /locus_tag="VFH83_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET6487298.1"
                     /translation="GFYEFTLEKVPLSVITRSWLLENVRSGSGRGFQTFKRAMDILVS
                     VVLLLVSLPLWPIISAAIKLESPGPVLFRQERLGKDRRRFTILKFRTMRVNGNNHTPT
                     EKKDARVTALGKLLRRTRLDEIPQALNILKGEMSVVGPRPERPELAETLAREIPFYYE
                     RNQVLPGITGWDQVCGEYHSPSSSDTYKKIQYDLYYIKNQSVGLELKILLKTVRTMLL
                     REGR"
     gene            953..2761
                     /locus_tag="VFH83_12805"
     CDS             953..2761
                     /locus_tag="VFH83_12805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6487299.1"
                     /translation="MSSSAILVIADDPINRKILRELLTFGRYRALEATDPETGLQVAR
                     DAQPDCILLDLALPGLDIPSSARNLKAAARRAPLIAVTGREMTGDQRKALDADFDGFV
                     TRPIDTRTFLGILGQLSRRAQSRGPEEGEEQPTARPRVHIGEGDPAEMSRLARVLRME
                     GYDVSGAPEGESLLEMAILTTPDLLLVDAGLPGLDGFEVTRRLKSNRSTLHVPIVLAS
                     AGHSGDHWARALEAGADEFLSKPVNPAELLALTRSMIRTKRSGERLASPAMPGDLTAS
                     ISAMEPVSEPTPPVVAILGPRREDVEVLERDLADRGSRVLWARSQEEAFSLASSRSVD
                     VIVLRPEECSPQTLAVCRSVKQREQSIQLIVLCTGKDPGSRVAALEAGADDCLPVPYD
                     RTELSARVALLARRKARLDALQDRCRSALNAAASDPLTGLHNSTSFQWLLDMEIKRAL
                     RHSHPVSLILLDVDDLRKVNDRLGHLVGDLLLTELAQVLKESGRETDLIARYTGDEFA
                     LALPHTGFDGACLLAERLRRSICANPFLQGRLNAGEVTVSLGVAAIPTDAATFEDLLR
                     TADERLCRAKRGGKNRACVEAEPSPAEEPSLADVTV"
     gene            2848..>3368
                     /locus_tag="VFH83_12810"
     CDS             2848..>3368
                     /locus_tag="VFH83_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6487300.1"
                     /translation="MEKARIMIVEDEKLVALSIEKCLKTMGYEVPLTVSTGEEAVQKV
                     LELEPDLVLMDIRLKGAIDGVEAANRIKTRYKIPIVYLTAYSEDSTLERAKITEPFGY
                     LTKPFEERTLQATVEMALYRATMQGKLRRNKEKLETILQCIAEGVVVTSVNGTIEYLN
                     PAAQRLLLRDETLS"
BASE COUNT          617 a         1093 c         1135 g          523 t
ORIGIN      
        1 ggcttctacg agttcaccct ggagaaggtc ccgctcagcg tcattacgcg gagttggctc
       61 ctggagaacg tccggtccgg gtccggccga gggtttcaga cgttcaagcg cgcaatggat
      121 atcctggtgt ctgtggtgct cctcctggtg agtcttcccc tgtggcccat catcagcgcg
      181 gccatcaagc tggaaagccc gggccccgtg ctgttccgcc aggaacggct tggaaaggac
      241 cgccgccgat tcaccatcct gaagttccgt accatgcggg tgaacggcaa caatcacacg
      301 ccgacggaaa agaaggacgc gcgggtcacg gcgctgggga agctgttgag acggacccgc
      361 ctggacgaga tcccgcaggc gctcaacatc ctcaagggag agatgagcgt ggtgggtccc
      421 cggccggagc gccccgagct ggcggaaacc ctggcgcggg agatcccgtt ctactacgag
      481 aggaaccagg tgctcccggg gatcaccgga tgggaccagg tgtgcggcga gtaccactct
      541 ccgtccagct ccgataccta caagaagatc cagtacgacc tgtactacat caagaaccag
      601 tccgtaggcc tggagctgaa gatcctcctg aagaccgtcc gtacgatgct cctgcgggaa
      661 ggccggtagg acctcgaccc cgatcggccg tcaccgcaag gcccgtaggc cctgagggag
      721 cgccggttcc caccaagggt ctgccaggaa atctctgaaa caagcccgaa aagccccgca
      781 aaatggtgga attactccgg tgtcgatata attgagattg cgggaacagc ggtagaaacg
      841 gacaaactca gggcgggaac cgggcccaat gcataaggtg gagcatgccg cgcaggaaga
      901 ccgttcacca aagtcgctgt caggtcccag atctcggcct cggaggagta tcatgagctc
      961 gagcgccatt ctcgtcatcg ctgacgaccc gatcaaccgc aagatccttc gagagctgct
     1021 caccttcggg cggtatcgag ccctggaggc cacggacccc gagacggggc ttcaggtagc
     1081 ccgggatgcg cagccagact gcatcctcct ggatctggct cttccggggc tcgacatccc
     1141 atcctccgcc cgaaacctca aggcggcggc gcgccgcgcg ccgctgatcg ccgtgacggg
     1201 gcgagagatg accggtgacc agcgcaaggc cctggatgcc gacttcgacg gcttcgtgac
     1261 ccggcccatc gacacgcgga cgttcctggg catcctgggc cagctatcgc gcagggcgca
     1321 gagccgcggc ccggaagagg gggaggagca gccgacggct cgaccgcgcg tccatatcgg
     1381 tgagggcgat cctgcggaga tgagccggct ggcccgggtg ttgcggatgg aggggtacga
     1441 cgtatcgggt gccccggagg gggagagcct cctggaaatg gccatcctta cgaccccgga
     1501 cctccttctg gtggatgcgg gtctgccggg gctggacggg ttcgaggtca cgcggcggct
     1561 gaagagcaac cggagcacgc tccacgtgcc aatcgtgctg gcctccgcgg gtcactcggg
     1621 ggatcactgg gcccgcgcgc tggaggccgg cgccgatgag ttcctgtcca agccggtcaa
     1681 cccggcggag ctcctggcgc tcacccggtc catgatccga acgaagcgca gcggagagcg
     1741 gctggcttcg cccgcaatgc ccggcgacct tacggcctcc atctccgcga tggagccggt
     1801 ttccgagccg acgcctccgg tggtcgccat cctgggtccg cgtcgggagg acgtcgaggt
     1861 cctggagagg gacctggcgg atcgcggctc gcgggtcctg tgggcccgaa gccaggagga
     1921 ggcgttttcc cttgcgtcca gccggtcggt ggacgtgatc gtcctgaggc ccgaggagtg
     1981 ctccccgcag actctggcgg tctgcaggag cgtcaagcaa cgggagcagt ccatccagct
     2041 catcgtcctc tgcaccggta aggaccccgg ctcccgggtg gcggccctgg aggcgggagc
     2101 cgatgactgc ctgccggtcc cctacgatcg aacggagctg agcgcccggg tggcgctcct
     2161 ggcgcgccgc aaggcgaggc tcgacgcgct ccaggaccgt tgccgctccg cgctcaacgc
     2221 cgcggccagc gacccgctga cgggtctgca caacagcacc tcgttccagt ggctgctgga
     2281 catggagatc aagcgcgcgc tgcgccactc ccacccggtt tccctgatcc tgctggacgt
     2341 ggacgacctg cgcaaggtga acgatcggct cgggcacctg gtgggggacc ttctgctcac
     2401 ggagttggcg caggtcctca aggagagcgg gagagagacc gacctgatcg cgcgctacac
     2461 gggcgacgag ttcgccctgg cgctcccgca cacggggttc gatggcgcct gtctcctggc
     2521 ggagcgcctg cgccggtcga tctgcgccaa ccccttcctc cagggccgcc tcaacgcggg
     2581 ggaggtcacc gtgagcctgg gcgttgccgc catcccaacg gacgcggcca ccttcgagga
     2641 cctcctgcga acggcggatg agaggctctg cagggccaaa cggggcggaa agaaccgcgc
     2701 ctgcgtggaa gcggagccgt cccccgcgga ggagcccagc ctggcggacg tgaccgtata
     2761 gccggcggcg ttccgtgcgt ctggaacccc gttccggcgc gtggtatctt tcgaagtaac
     2821 actggggtcg gatcgaggtg gggaactatg gagaaagcgc gcatcatgat cgtggaggac
     2881 gagaagctgg tcgccctctc catcgagaaa tgcttgaaga ccatgggata cgaggtcccg
     2941 ctcaccgtct cgacgggtga ggaagcggtc cagaaggtcc tggagctgga gccggacctc
     3001 gtgctcatgg acatccggct gaaaggcgcc atcgacgggg tggaagccgc caaccgcatc
     3061 aagacccgct acaagatccc tatcgtctat ctcacggcct actccgaaga ctccaccctg
     3121 gagcgagcca agatcacgga gcccttcggg tatctcacca agccgttcga ggaacgcacc
     3181 ctccaggcta cggtggagat ggcgctgtac cgcgccacca tgcagggcaa gctccgccgc
     3241 aacaaggaga agctggagac catcctgcag tgcatcgcgg agggcgtggt ggtcaccagc
     3301 gtcaacggca ccatcgagta tctcaacccc gccgcccagc ggctgctcct gcgggacgag
     3361 acgctgtc
//
LOCUS       DASRZS010000525         4930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_292972, whole genome shotgun sequence.
ACCESSION   DASRZS010000525 DASRZS010000000
VERSION     DASRZS010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292972"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VFH83_12815"
     CDS             complement(<1..702)
                     /locus_tag="VFH83_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487301.1"
                     /translation="MSPPAPALEALYRDRLARYLAAMRGGQPDRVPIRLFVEEFAARY
                     AGYSNYQTACDYTLAFEATRRCVSELGCDAAMSNGIVNWMGMSKAIGWKPIRFPGLGL
                     PVQSATQWTEPSDEAGAFLKLDEYDELADDPTGFLVNRWLPRFTDHLRAPGEPVTYNH
                     TMALINGALSFQTYLSAFGTYGDRLRILSGVVPATAGTLKAPLDILGDKLRGFLNLSI
                     DLMERREKVIQACEAL"
     gene            complement(731..1591)
                     /locus_tag="VFH83_12820"
     CDS             complement(731..1591)
                     /locus_tag="VFH83_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6487302.1"
                     /translation="MSKQATQSQRREKGSRGLAVGVSVVLWFLCATYVYPFVWMFYNS
                     FKTEAAYRSNPYNLPLPPTVQAYQSIFARHDVYLAFLNSLFNTVCAIVGIVVLSFLVA
                     YFLGRYRFRGRTFLYGFFMAGLFVPGLGLLIPSYIQFHTLGMIGHWYTLLLPYITGGI
                     PTAIYLFDSYIRTIPRSLEEAAHMDGATITDIIRLIMFPMCLPMTATIVVINFLGYWN
                     EFPLALLMQPSPKFRTMTVWLSMFQSMYEANLTGKLTAMFILTLPVIITYFLFREQMM
                     RGLASGAIKG"
     gene            complement(1588..2487)
                     /locus_tag="VFH83_12825"
     CDS             complement(1588..2487)
                     /locus_tag="VFH83_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6487303.1"
                     /translation="MAFQRRPKTLYDISFLKALPFLALPIGLYVFMMIVPLLGAFYFS
                     LNHIFGNRFTFVGLRNFQHLLSDPIVLLALKNNAIFILVSIVFQVGPAFIIMSLIATR
                     LVYGSNAVRAIFFFPVVVSPLVTAYVWKIMYNPDFGVLNGIIRALGAHNFQRDWLSDP
                     KFIITSISIPLAWQYIGYYLILLLAGFTSIDPGLLEAAEIDGATGGQRTRFIIWPLMR
                     NTLNVCLLLCVSGGLNIFSQVYAMSGGGPGYTSTVLSMYAFTNSFENADFGYGATISI
                     SMLIIGFAVVVVLLTLRRNTEAE"
     gene            complement(2653..4032)
                     /locus_tag="VFH83_12830"
     CDS             complement(2653..4032)
                     /locus_tag="VFH83_12830"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6487304.1"
                     /translation="MSRRKLILLGACAWLGLFLLVAAPGAFAGGQGEKANGPVQFAFG
                     STWAPSQSGQAEDYHLGIFKAWQAKNPNVKVDLQEYGSADYVQIQEKTWLASNTWPDS
                     FEINTVDLATAAKTGQLLDITSALNADTAWRDSFLPGMVANENLIGGKYYGVPMQFIT
                     NETFFYNTDILKQAGYTEFPKTWGDLLALCAKLKSMGYVPIAMVDKATWPAVSHLLEP
                     LCQFIMGPDWVKKVGAFSADASYNDPRFLDVLKKINDLKTLGYLPSNVASADFGDDTG
                     LYFAKKAAIYLDGSWAVSGVIKNAPADVLAVTKAAPMPRPENSDPASAYGLFTGGSGW
                     SYAGRADLAKATDAKRNAVLSLIKFMTGPDAIGTIVQNYQLPPNKLPSTLDMSKTATL
                     MVDTFKMFSASPSIPPMNQQQNGATMSTALYKDCQAMLAGAMTPEQMMTDVQAAYVKA
                     VAEMAAINK"
     gene            4258..>4930
                     /locus_tag="VFH83_12835"
     CDS             4258..>4930
                     /locus_tag="VFH83_12835"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET6487305.1"
                     /translation="MWELTILDANLPAQRLDSLAAADLDGDGALELVTGGDGLFWVRP
                     RDRAWGRISPRYAHVGVVVGDIDGDGKPEVIVGEGEALPDKRAELRFGGAGIHSRTKD
                     WSIAWYKPGADLRGPWATGLIDPRFEGHVHDILLADIDGDGTRELIAISCYSDHPGIF
                     ILKPGRDPHRPWTKHAVSEGIFTEGLSIGDLDGDGRLEIVCGPDWYKAPLAGPLSGHW
                     TRHTFA"
BASE COUNT          956 a         1479 c         1626 g          869 t
ORIGIN      
        1 cagggcctcg caggcctgga tgactttctc ccggcgctcc atgaggtcga tggagaggtt
       61 caggaacccg cgaagcttgt ctcccaggat gtccaacgga gccttgaggg tgccggcggt
      121 agcgggtacg acaccgctca ggatccgaag gcgatcgccg tacgtgccga atgcggacag
      181 gtaggtctgg aacgagagcg ccccgttgat gagggccatg gtatggttgt acgtgaccgg
      241 ctctccggga gctcggaggt ggtccgtgaa ccgaggcagc cagcggttga ccaggaaccc
      301 ggtgggatca tccgccagct cgtcgtactc gtcgagcttc aggaacgcgc cggcttcgtc
      361 ggatggctcc gtccactgcg tggcgctctg gaccggaagt ccaagacccg ggaaccggat
      421 gggcttccag ccgatggcct tgctcatgcc catccagttc acgatcccat tgctcatggc
      481 cgcgtcacat cccagctccg aaacgcagcg gcgcgtcgct tcgaaggcca gggtgtagtc
      541 gcaggcggtc tgatagttgc tatagccggc gtagcgggcc gcgaactcct cgacgaagag
      601 gcgaatgggg acgcgatccg gctgcccgcc gcgcatggcc gccaggtagc gtgccagacg
      661 gtcccggtag agcgcctcca gcgcgggcgc cggggggctc accgtgcgct ccccgcacgg
      721 cagggctcgc tcatcccttt atggcgccgg aagcgagccc ccgcatcatc tgctctcgga
      781 agaggaagta ggtgataatg accgggagcg tcaggatgaa catggccgtg agcttgccgg
      841 tcaggttcgc ctcgtacatc gattggaaca tggagagcca tacggtcatg gtgcggaact
      901 tcggactcgg ctgcatgagc aacgccagag ggaactcgtt ccagtacccc aggaagttga
      961 tcaccacgat cgtggccgtc ataggcaggc acatcggaaa catgatcagc cgtatgatgt
     1021 ccgtgatggt cgcgccgtcc atgtgcgccg cctcttcgag ggacctcggg atggtgcgga
     1081 tgtagctatc gaagaggtag atggccgtgg gaatgccgcc cgtgatgtag ggcagcaaga
     1141 gcgtgtacca gtggccgatc atcccgagcg tgtggaactg gatgtagctc gggatcagca
     1201 gacccaggcc gggcacgaac aggccggcca tgaagaaccc gtagaggaag gtccgacccc
     1261 ggaatcggta gcgacccagg aagtaggcaa cgaggaacga caggacgacg atgccgacga
     1321 tggcgcatac cgtgttgaag aggctgttga ggaacgcgag atacacgtcg tgccgggcga
     1381 agatgctctg gtaggcctgg accgtcgggg gcagcggcag gttgtagggg ttcgaccggt
     1441 aggctgcctc ggtcttgaac gagttgtaga acatccagac gaacgggtag acgtacgtgg
     1501 cgcacaggaa ccagagcacc acgctcacgc caacggcgag cccccggctg cccttctccc
     1561 gtcgttgcga ttgggttgct tgcttgctca ttcggcttcc gtgttccgac gcagggtgag
     1621 cagcacgacc accaccgcga atcctatgat gagcatggag atcgagatgg tcgcgccgta
     1681 cccgaaatcc gcgttctcga acgagttcgt gaacgcgtac atcgacagta ccgtcgaggt
     1741 gtacccgggc ccgccgcccg acatggcata gacctgagag aagatgttga gcccgcccga
     1801 cacgcagaga agcagacaga cgttcaacgt gtttctcatg aggggccaga tgatgaagcg
     1861 ggtgcgctgg ccgccggtgg cgccgtcgat ctcggccgct tccagcaggc cagggtcgat
     1921 cgacgtgaag ccggccagga ggaggatcaa gtagtacccg atatactgcc aggccagcgg
     1981 gatcgagatc gaagtgatga tgaacttcgg atccgagagc cagtccctct ggaagttgtg
     2041 cgcccccagc gcccggatga tcccgttcaa cacgccgaag tcggggttgt acatgatctt
     2101 ccagacgtac gcggtcacga ggggagagac gaccacgggg aagaagaaga tggcccggac
     2161 ggcgttgctc ccatagacca gccgcgtcgc gatgagcgac atgatgatga acgccgggcc
     2221 gacctggaag acgatcgata ccaggatgaa gatcgcgttg ttcttcaagg cgaggagcac
     2281 gatcggatcg ctcaagaggt gctggaaatt cctgagcccg acgaaggtga accggttgcc
     2341 gaagatgtgg ttcaggctga agtagaacgc tccgagcagc ggaacgatca tcatgaacac
     2401 gtacagcccg atcggcaagg ccaggaacgg cagggccttc agaaaggaaa tgtcatagag
     2461 cgtcttgggt cgtctctgga aagccacgct atctctccgc cgggtttcca cgatggtgcc
     2521 aaacagggcg cgttcgccgg ggaaggcaaa cgcgcccgta cgcaaccctg atagcccggc
     2581 ctcgacggta ccacgtcgat cgcagaacct gcaacgaccg ccgggcgtca gcggtcagga
     2641 acgccgtacc ctctacttgt tgatggccgc catttcggca acggccttca cgtatgcggc
     2701 ctgcacgtcc gtcatcatct gctcgggcgt catggcgccc gcgagcatcg cctggcagtc
     2761 cttgtacagc gccgtggaca tcgtcgcgcc gttctgctgc tggttcatcg gcgggatgga
     2821 cggggaggcg gagaacatct tgaacgtatc caccatcaac gtcgccgtct tggacatgtc
     2881 cagggtggag ggcagcttgt tcggcggaag ctggtagttc tgcacgatgg tcccgatcgc
     2941 atccgggccc gtcatgaact tgatcagcga aagcacggcg ttgcgcttcg catccgtagc
     3001 cttggccagg tccgcgcgcc cggcgtagga ccaaccggag cctccggtga acaggccata
     3061 cgcgcttgcg gggtccgagt tctcgggacg aggcatcggc gccgccttgg tgacggccag
     3121 gacgtcggcc ggcgcgttct tgatcactcc gctcacagcc caggaaccat ccaggtaaat
     3181 ggccgccttc tttgcgaagt acagacccgt gtcatctccg aagtccgccg aggccacgtt
     3241 ggagggcagg tagccgaggg tcttcaggtc gttgatcttc ttcaaaacat cgaggaaccg
     3301 cggatcgtta tagctggcgt ccgcgctgaa ggcgccgacc ttcttgaccc agtccgggcc
     3361 catgatgaat tggcagagcg gctcaagcag gtgcgacacc gcgggccagg tggccttgtc
     3421 caccatcgcg atggggacgt atcccatgct cttcagcttg gcgcacaggg cgagcaggtc
     3481 tccccacgtc ttggggaact cggtgtatcc ggcctgcttc aggatgtccg tgttgtagaa
     3541 gaacgtctcg ttcgtgatga actgcatcgg gacgccgtag tacttgcctc cgatcaggtt
     3601 ctcgttggcc accattccgg gaaggaacga gtccctccag gcggtgtccg cgttgagggc
     3661 ggaggtgatg tccaggagct gaccggtctt cgcggccgtg gcaaggtcaa cggtgttgat
     3721 ctcgaacgag tcgggccacg tgttggatgc cagccaggtt ttctcctgga tctgcacgta
     3781 atcggcgctg ccgtattcct ggaggtcaac cttgacgttc gggttcttcg cctgccaggc
     3841 cttgaagatg ccaaggtggt agtcttccgc ctgtccactc tggctcggtg cccaggtgct
     3901 cccgaacgcg aactgtacgg gtccgttcgc cttctctccc tggccacccg cgaaagcgcc
     3961 aggcgccgcg acaagcagga acaagcccag ccatgcgcag gcacctagca agatcagctt
     4021 ccttctcgac atctcagaca cctcctaatg tcataatgtt aagaccacat gccaataaac
     4081 catagttcct tgcacctgtc aatactccac ccgaccggct tccagccctg gcgaatggcc
     4141 agcttgccgc agaagcaggg gcggaggtaa gctacgcggc acgcacggcc cgacggcgca
     4201 gcagcggacg agacctctcg ggactccgac cggggcgaca agaatggggg caaatcgatg
     4261 tgggagctga cgatcctgga cgcgaacctg ccagcccaga ggctggatag cctggcggcg
     4321 gctgatctcg acggagacgg cgccctggag ctggtgacgg gcggggacgg actgttctgg
     4381 gtgcggccgc gagatcgggc ctggggccgg atcagccccc gctacgcgca cgtcggcgtg
     4441 gtggttggcg acatcgacgg ggatgggaag cccgaggtga tcgtcgggga gggagaggcc
     4501 cttcccgaca agcgggcaga gctgcggttc ggtggagcgg gcatccattc caggaccaag
     4561 gattggagca tcgcctggta taagccgggc gcggacctgc gcggcccgtg ggccaccggc
     4621 ctcatcgacc cgaggttcga gggccacgtc cacgacattc tccttgccga catcgacgga
     4681 gacggcactc gggagctcat cgccatctcg tgctattccg accatccggg gatcttcatc
     4741 ctgaagcctg ggcgggatcc gcatcgtccg tggaccaagc acgccgtgtc ggaaggcatc
     4801 ttcaccgagg ggctctccat cggagacctc gacggcgacg gtcgtctgga gatcgtctgc
     4861 ggccccgact ggtacaaggc tcccttggcc ggaccgcttt ccgggcactg gacgcgccac
     4921 accttcgcaa
//
LOCUS       DASRZS010000526         4597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_673261, whole genome shotgun sequence.
ACCESSION   DASRZS010000526 DASRZS010000000
VERSION     DASRZS010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673261"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VFH83_12840"
     CDS             <1..898
                     /locus_tag="VFH83_12840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET6487306.1"
                     /translation="FSVSQPGSVALHPRVALPTEGRPGDLLRLEVDAGSVMTSASASL
                     SNSGGRVMTRAAGFRFSGSAGAERWAALLGIPAGLEPGDYMLRLTARADASTYLVLTA
                     FTVQPRDFKNETIALTQQLTSLRTAPDPRKTAEANHLEAIVTTPHADALYDPLSFVLP
                     LTGTHRSSGYGDRRTYEYSGAGDEVSIHEGVDIAAPSGSPIVACGRGRVIFAGSFLMT
                     GWTVVIEHLPGLFSLYFHMSALEVRQGQEVETGQEIGKVGMTGFATGPHLHWEVESLG
                     VSVDPDLLVGSPLLPAPAGSAP"
     gene            964..1173
                     /gene="rpsU"
                     /locus_tag="VFH83_12845"
     CDS             964..1173
                     /gene="rpsU"
                     /locus_tag="VFH83_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET6487307.1"
                     /translation="MAYIEVSEDEPLEKAIKRFKRQVEKEGIIREWKKREFFEKPSAI
                     LNRKKKALERKRLKRARKVSQMKSY"
     gene            complement(1275..2006)
                     /locus_tag="VFH83_12850"
     CDS             complement(1275..2006)
                     /locus_tag="VFH83_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET6487308.1"
                     /translation="MQDRVRIIHASKNFGKVVALRDATLSVKPGEVVLIIGPSGSGKS
                     TMLRCINRLENLTSGEIWIENDQVTDRQSDIRRIREEVGMVFQSFNLFPHLTALGNIT
                     LAPRVVKKMPESEASDLGRSLLARVGLSEKEHSFPDQLSGGQQQRVAIARALAMSPKV
                     MLFDEPTSALDPEMIKEVLDVMVELAREGMTMLVVSHEMGFARAAAHSVVFMDNGEII
                     ERGTPDELFSHPKEERTQRFLKHIL"
     gene            complement(1996..2796)
                     /locus_tag="VFH83_12855"
     CDS             complement(1996..2796)
                     /locus_tag="VFH83_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET6487309.1"
                     /translation="MAEVDREPQRETRIEVTDGALIPEKGEKPTFSAWRISFFGAILL
                     LILLPILHPDPYWKILLFIPDGIARTFEVTVLAICFAVVVGLIAGVGRISRITILNRI
                     ATVYVEVIRGIPLLVQLFYIYYALGRVVHVPDIFAAIIAMTVCYGAYIGEIFRAGIQS
                     IPKGQMEAAIALGLTRGQALRRVILPQTVKVVLPPLGNEFIALLKDSSLVSILAVSDL
                     LRRGREFAARTFEYFEVLTIVALVYLVMTLFFSRLVALMEERLRRRAR"
     gene            complement(2784..3542)
                     /locus_tag="VFH83_12860"
     CDS             complement(2784..3542)
                     /locus_tag="VFH83_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HET6487310.1"
                     /translation="MLKRLFIVVLLFAALGSFAFAGGKKEASKIVIATDATFPPMEMV
                     DENKNIVGFDIDLMNAAAKAAGFTVEFRNTQWDGIFVGLADKKYDAVMSSVTITDDRK
                     KTMDFSIPYVNAGQILVVPVATTGVTTLADLSGKAVGAQIGTTGAFEIDKVKDQYKIN
                     EKTYDDEGLAFADLANGAIAAVVCDTPVAADYALQEKDYKGKFKIVGGQFTQEFYGVA
                     VVKGNSAILDQINKGLKAVIDSGEEKTIEAKWLK"
     gene            3654..3726
                     /locus_tag="VFH83_12865"
     tRNA            3654..3726
                     /locus_tag="VFH83_12865"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3687..3689,aa:Met,seq:cat)
     gene            complement(3785..>4597)
                     /locus_tag="VFH83_12870"
     CDS             complement(3785..>4597)
                     /locus_tag="VFH83_12870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487311.1"
                     /translation="MLGVGNTEAGHTLNDGTLLDFAMIRYNADGTVDASFGNNGRVST
                     DFGYPGDGTDSAMAGVIVLPDGSILLGGLSQNGPDTQRNRGFSVAKYDSSGNLIASFG
                     NNGRQLIALGLDVAEGRGMCVDSLGRIVLVGEANTIPGAYYNSYAIVRLTANGQPDAS
                     FGSGGTVIYPFGGLDTYACAVKALPDTSLVVGGAIGDTSGTNYAKLSVLFLNPDGSRN
                     TSLGTAGVLSIDTGVATLEGLAQSMALGPDGSIYLTGNYNWAPMSIVVVKIH"
BASE COUNT          836 a         1462 c         1444 g          855 t
ORIGIN      
        1 gttctccgtt tcccagcccg gctcggtggc gctccatccc cgggtcgccc ttcctacgga
       61 ggggcggccg ggcgacctgc ttcgcctgga agtcgacgcc gggtcggtga tgacttccgc
      121 gtctgcgtcg ctctcgaaca gcggcggacg cgtgatgacc agggccgccg ggttccggtt
      181 ctccggatcc gccggcgcgg agcgttgggc tgccctgctg gggatcccgg ccggccttga
      241 gcccggagac tacatgcttc gccttaccgc ccgcgcggac gccagcacgt acctcgtgct
      301 gacggcgttc accgtccagc cgcgggactt caagaacgaa acgatcgcgc tcacccagca
      361 gctcacgagc ctgaggacgg ctccggatcc gcggaagacc gccgaagcaa accacctgga
      421 ggcgatcgtt acgactcccc acgcggacgc cctgtacgat ccgctctcgt tcgtgttgcc
      481 gctgacgggg acgcaccgca gctcggggta tggcgaccgc cggacctacg agtactccgg
      541 cgccggcgac gaggtctcga tccacgaggg tgtggacatc gccgccccca gcgggagccc
      601 gatcgtggca tgcggtcgcg gtcgagtgat cttcgccggt tcgtttctga tgaccggctg
      661 gaccgtcgtg atcgagcatc tgccgggcct cttctcgctc tacttccata tgtccgctct
      721 ggaggtgcga cagggccagg aggtggagac cggccaggag atcggaaaag tcgggatgac
      781 gggctttgcc acgggtccgc acctgcactg ggaggtggag tccctggggg tctcggtgga
      841 cccggacctt ctcgtcggca gcccgttgct gccagcgcct gccggctcgg cgccttgaca
      901 aagcgccgcc gttgaccaat attggtggta atcctgacac tcgcagggaa ggaggtgatt
      961 ccaatagcct acatcgaagt cagcgaggat gagcccctcg agaaggcgat caagcgcttc
     1021 aagcgccagg tcgagaagga ggggatcatc cgggaatgga agaagcggga gttcttcgag
     1081 aaaccgtccg ccattctcaa tcgcaagaag aaggctctgg agcgaaagcg cctgaagagg
     1141 gcgcgaaaag tctcccagat gaagagctac taagccttcc cgaccgaaat caaaagggcc
     1201 cctgttccgc ggcggccgcg gggcaggggc tctttccttt ggcacgcgga gaatgccggc
     1261 gtgcccgtcc tctgctaaag gatgtgcttg agaaaccgct gagtccgctc ttccttgggg
     1321 tgcgagaaga gctcgtccgg agtgccccgc tcgatgatct cgccgttgtc catgaacacc
     1381 accgagtgcg ctgcggcgcg ggcaaacccc atctcgtggg aaaccacgag catcgtcatt
     1441 ccttcccgcg ccagctccac catgacgtcc aggacctcct tgatcatctc gggatccagg
     1501 gcggaggttg gctcatcgaa gagcatgacc ttggggctca tggccagggc tcgggcaatc
     1561 gccacccgct gctgctggcc gccggagagc tggtcgggga aggagtgctc tttctcggag
     1621 agcccgacgc gggccagcag gctcctgcca aggtccgagg cctcggactc cggcattttc
     1681 ttcaccaccc gcggcgccag cgtgatgttg ccgagggcgg tcaggtgcgg aaaaaggttg
     1741 aaggactgga agaccatccc aacctcttct cggatcctgc ggatgtcgga ttggcggtcc
     1801 gtgacctggt cgttctcgat ccagatctcc ccggaagtga ggttctccag ccggttgatg
     1861 cagcgcagca tcgtgctctt tccgctgccc gagggtccga tgatcagcac gacctctccc
     1921 ggcttgaccg agagggtggc gtcccgaagc gccaccactt ttccgaagtt cttgctggcg
     1981 tggatgatcc gaaccctatc ttgcacggcg gcgcaacctt tcctccatca gcgcgaccag
     2041 cctcgagaag aagagggtca tcacgaggta gaccagcgcg acgatggtca gcacttcgaa
     2101 gtactcgaag gtccgcgcgg cgaactctcg tcctcgccgc aggagatccg acaccgccag
     2161 gatggagacc agggaggagt ccttcagcag ggcgatgaac tcgttgccga gcggaggcag
     2221 gacgaccttc acggtctggg gcaggatgac gcgtcggaga gcctggccgc gcgtgaggcc
     2281 cagggcgatg gccgcctcca tctgtccttt cgggatggac tggattccgg cccggaagat
     2341 ctctccgatg tacgcgccgt agcagaccgt catggcgatg atcgccgcga agatgtccgg
     2401 gacgtgcacc accctgccca gagcgtagta gatgtaaaag agctggacca gcaggggaat
     2461 gccgcggatc acttcaacgt acaccgtggc gatccggttc agaatcgtga tccgagagat
     2521 gcggccgacg ccggcaatca ggcccaccac cacggcgaag cagatggcaa ggaccgtgac
     2581 ctcgaacgtc cttgcgatcc cgtccgggat gaagagcagg atcttccagt acgggtccgg
     2641 gtggagaatg gggagcagaa tcagcagcag gattgccccg aaaaaggaga tccgccaagc
     2701 cgagaaggtc ggcttctccc ccttttcggg gatcagcgct ccgtcggtaa cctcaatacg
     2761 cgtctcgcgt tgaggctccc ggtctacttc agccacttgg cctcgatggt cttctcctcg
     2821 ccggagtcta tgaccgcctt gagacccttg ttgatctgat ccaggatcgc ggagttgccc
     2881 ttgacgaccg ccaccccgta gaactcctgc gtgaactgac cgccgacgat cttgaacttg
     2941 cccttgtagt ccttctcctg gagggcgtag tccgccgcca cgggcgtgtc acagaccacc
     3001 gccgcgatgg ccccgttggc cagatcggcg aacgccaggc cctcatcgtc gtacgtcttc
     3061 tcgttgatct tgtactggtc cttcaccttg tcgatctcga aggcaccggt ggtgccgatc
     3121 tgcgcgccaa cggcctttcc gctgaggtcc gccagggtcg tgacgccggt ggtcgccacg
     3181 ggtaccacga ggatctgccc cgcgttgacg tatgggatgg agaaatccat ggtcttcttg
     3241 cggtcgtccg tgatggtcac ggaggacatg accgcgtcgt acttcttgtc cgccagtccg
     3301 acgaagatcc cgtcccactg ggtgttgcgg aactcgaccg tgaacccggc cgccttggcc
     3361 gcggcgttca tgaggtcaat gtcgaagccg acgatattct tgttctcgtc gaccatctcc
     3421 atgggaggga aggtggcatc cgtggcgatc acgatcttgg acgcttcctt ctttccgccg
     3481 gcgaacgcga agctgccgag cgcggcgaac agaaggacca cgatgaagag cctcttgagc
     3541 atgggtacct ccttgcgaat tgttgattca ctatcctcca ccccgcgacg gcctgtcaag
     3601 aaggagagcc acttgacgtg ggccggcctt tgcgcctata ctcttgttca cgacgcaggg
     3661 tagagcagtt ggaagctcgt cgggctcata acccggaggt cgcaggttca aatcctgccc
     3721 ctgctaacga gacggcgcga accggtcgac ggtaacgcgc cgttgttcat tgcgccgtcc
     3781 tccatcaatg aatcttcacc acgacgatcg acatcggagc ccagttgtag ttgccggtga
     3841 ggtagatgct cccatccggg ccaagcgcca tggattgcgc cagtccctcc agagtcgcca
     3901 ctccggtgtc gatggaaaga acccccgccg tgccgaggct cgtgttccgc gagccatccg
     3961 ggttcaggaa caggacggac agcttcgcgt agttggtgcc cgacgtgtcg ccgatggctc
     4021 cgcccacaac cagagaggtg tccggcaagg ccttgaccgc gcaggcatac gtgtccagcc
     4081 cgccgaacgg atagatcacc gtgccgccgc tgccaaagga cgcgtccggc tgcccgttcg
     4141 cggtcaggcg aacaatcgcg taggaattgt aataggcccc cggtatcgta ttggcctcgc
     4201 cgaccaggac gatcctgccg agcgagtcca cgcacatgcc tcttccttcc gccacatcga
     4261 ggcccaatgc aatgagctga cggccgttgt tcccgaacga cgcgatcagg ttcccgctgg
     4321 aatcgtattt cgcaacggag aagccgcggt tcctctgggt atccggaccg ttctgcgaca
     4381 atccgcccag gaggatggat ccatccggca ggacgatcac gcccgccatg gcgctgtccg
     4441 tgccgtcacc ggggtaaccg aagtccgtgg agaccctgcc gttgtttccg aaagaggcgt
     4501 cgacggtgcc gtcggcgttg tatcggatca tggcaaaatc gagcagggtc ccatcgttca
     4561 gcgtatggcc ggcctccgta ttcccgacgc cgagcat
//
LOCUS       DASRZS010000527         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_673553, whole genome shotgun sequence.
ACCESSION   DASRZS010000527 DASRZS010000000
VERSION     DASRZS010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673553"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFH83_12875"
     CDS             <1..474
                     /locus_tag="VFH83_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HET6487312.1"
                     /translation="PNLDGAGNYVILEASGKGHYVGCHLDVDCFAPAKNDWWGEGDDM
                     VFIDGEPLPRLYGTGSEDYFNTAFGPTQEYSSPYHGLTVNSGTKEWPWKGKNSVYRYH
                     IEDPIHFEKSIRVTIEHGEANCLSLDMASTAYWYQTEPHGPFPALLPVQDRLPRP"
     gene            471..959
                     /locus_tag="VFH83_12880"
     CDS             471..959
                     /locus_tag="VFH83_12880"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HET6487313.1"
                     /translation="MIRVIITGGTFDKHYDAVRGYLTFKETHLPEILSFVRCSVPIQL
                     ELNQLIDSLDMQTANRLAILEACRRAPEERIVITHGTDTMVETGRVLGEAGLEKTIVL
                     TGAMVPYIFNNSDAVFNLGCAITAVQLQPHGVSIAMNGRIFPWNRVRKNRAIGGFEDA
                     EG"
     gene            1135..>2015
                     /locus_tag="VFH83_12885"
     CDS             1135..>2015
                     /locus_tag="VFH83_12885"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HET6487314.1"
                     /translation="MPDLSTTYLGLTLKNPVIVGSSGITASLEGLKAAEAGGAAAVVL
                     KSIFEEEIAAEYQEALAAAKTKGMSLESYDYYDYEVRGNRVASAVELVRQAKAALRIP
                     VIASVNCTYSHEWASFARELEQAGADALELNMFFLPSDLRRSSEEREQEYFRIVERVL
                     AQIHIPVALKISPYFSTLAQMVERLSRTGVAGLVLFNRFFAIDFDVEKLKVTSTNMLS
                     DPVELALPLRWIALMARRVGCDLAGSTGVSDGKSVIKLVLAGAKAVQVVSALQRLGPE
                     HTAAICSEVDQWMSRHG"
BASE COUNT          372 a          676 c          649 g          318 t
ORIGIN      
        1 ccgaacctgg acggcgccgg caactacgtg atcctggagg ccagtggcaa gggccattac
       61 gtgggctgcc acctggacgt ggactgcttc gcgccggcca agaacgactg gtggggcgaa
      121 ggggacgaca tggtcttcat cgacggagag ccgcttccgc gcctgtacgg caccgggagc
      181 gaagactact tcaacaccgc cttcgggccg acccaggagt acagctctcc ttaccacggg
      241 ctcacggtga actccggtac caaggagtgg ccctggaagg gcaagaactc cgtgtatcga
      301 tatcacatcg aggacccgat ccacttcgag aagtcgatcc gggtcaccat cgagcatggg
      361 gaggccaact gtctcagcct ggacatggcc tccaccgcct actggtacca gacggagccg
      421 cacgggccgt tccccgcgct ccttcccgtc caggaccgcc tgccccggcc atgatccgcg
      481 tcatcatcac cggcggcacc ttcgacaagc actacgacgc ggtccgaggg tatctcacgt
      541 tcaaggaaac gcacctgccc gagatcctga gcttcgtgcg gtgctccgtg cccatccagc
      601 tcgagctcaa ccagctcatc gacagcctgg acatgcagac ggccaaccgg ctcgccatcc
      661 tggaggcctg ccgaagggcg cctgaggagc gcatcgtcat cacccacggg acggacacca
      721 tggtggagac gggccgagtg ctgggggagg cgggcctgga gaagaccatc gtgctgaccg
      781 gcgccatggt gccgtacatc ttcaacaact cggacgccgt gttcaacctc ggctgcgcca
      841 tcaccgccgt gcagctccag ccccacggag tctccatcgc catgaacggc cggatcttcc
      901 cctggaaccg ggtccgcaag aaccgggcca tcggcggttt cgaggacgcg gaaggatagc
      961 ctcggttcct gccggaaacg cgcggtagac cgcggccacc accacgtacg ctgaggctgc
     1021 gccgcggcac gctgaggctg cgccgcggta cgctgaggct gcgccgcggt acgccgcggc
     1081 gtccttgcgc agcagcgcga aagctgctat ccccttcgca aagaaggaga gcagatgccg
     1141 gacctcagca caacctacct ggggcttacc ctcaagaacc cggtgatcgt cggcagctcg
     1201 ggaatcacgg cctccctgga aggtctgaag gcggccgagg ccggcggcgc ggcggcggtc
     1261 gtgctgaagt cgatcttcga agaggagatc gccgccgagt accaggaggc gctcgccgcg
     1321 gccaaaacga agggcatgag cctggagtcc tacgactact acgactacga ggtccgcggg
     1381 aaccgcgtcg cctccgcggt ggagctggtt cgccaggcca aggcggccct gcggattccg
     1441 gtaatcgcca gcgtgaactg cacctattcc cacgagtggg cgtcgttcgc cagggagctc
     1501 gagcaggcgg gcgcggacgc gctggagctc aacatgttct tccttccctc cgacctgagg
     1561 cgctccagcg aagagcgcga gcaggagtac tttcggatcg tggagcgggt gctcgcgcag
     1621 atccacatcc ccgtggccct gaagatcagc ccgtacttct cgacccttgc gcagatggtg
     1681 gagcgcctct cgcgcacggg ggtggccggc ctcgtgctct tcaaccggtt cttcgccatc
     1741 gatttcgacg tcgagaagct gaaggtcacc tcgacgaaca tgctgagcga tcccgtcgag
     1801 ctggctctcc cgttgcgctg gatcgccctc atggcccgaa gggtcggatg cgatctggcc
     1861 gggtccaccg gcgtgagcga cggcaagtcg gtaatcaagc tcgtcctggc gggtgccaaa
     1921 gccgtccagg tagtctccgc gctccagagg ctgggacccg agcataccgc ggccatctgc
     1981 tccgaggtcg accagtggat gagccgacac ggcta
//
LOCUS       DASRZS010000528         3092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4520460, whole genome shotgun sequence.
ACCESSION   DASRZS010000528 DASRZS010000000
VERSION     DASRZS010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4520460"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VFH83_12890"
     CDS             <1..634
                     /locus_tag="VFH83_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET6487315.1"
                     /translation="HVNHTAEKPRYGVLMCVNGTGILYSWIRRLLFGDTGAVPYDRLN
                     QAAAEAPVGSDGMVILPYGNGAERTLENRNPGASFLDVDFNRHGIPHLLRSAQEGIAF
                     AFQYGLRVMRDLGIEVRTVKAGDANLFRSPLFREAFATVSGTRVELYETDGAQGAARG
                     AGVGAGIYRSPKEAFVGLAARGAVEPDPSKAGAYADAYGRWETELKRVLR"
     gene            complement(660..2294)
                     /locus_tag="VFH83_12895"
     CDS             complement(660..2294)
                     /locus_tag="VFH83_12895"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6487316.1"
                     /translation="MTARGVDGRNPDIVLKNGLLVDGSGARAYHANLLIRGGRIHRIS
                     RMPIRTTGLTIDCTGRVVAPGFIDAHSHLDWHVPIKGHDELKYPFVAQGITTVVAGNC
                     GTSAAGFRPSSSWKDRLADNMMANGFLSLQWDSVADYFERLASSGISHNLALLAGHGS
                     TRISIRGLDPSPLHPYEIKELLWLLEKAMAEGARGVSMGLQYEPGIYSRPEELREVAL
                     LVRRKGKILSVHLRAYSALAPGYPLRLGGEPHNLIALREILDLARSTGVRLEISHLIF
                     VGSRTWRTAEKALRLIDLAIAQGVDVKFDTYPYPFGASWINVVLPSWFLARGAAAYEE
                     TASLRKLKRQIFLIERLLGFGPAEVQVTDTRAEELAEYNGRFLNEIARVRRLSPVDAL
                     IDIAHRSGGRARVLCHRYSNDRILEELVRHPASLFMTDAWVDRGAVQNPAAYAAFPRL
                     LSLIRDRKLLSVEDGIRKMTGATAERFAITDRGLLKEGMAADIVVLDWEGIQDNTTGK
                     ETDKAPSGVEYVFVNGVKIIGSGKKENPLSAGVPLA"
     gene            2364..>3092
                     /locus_tag="VFH83_12900"
     CDS             2364..>3092
                     /locus_tag="VFH83_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6487317.1"
                     /translation="MIWVVGAVAIDLIAMRDRFLDGTSNPSDIRLGLGGVGYRVFSNL
                     DTPRRFITALAADPLSAWARKALESEEGVLIQAVEDRDAGPPLYLALMESGSLKVAAS
                     DFRIVEQQLSEDFVVRALQAGSPRDFLVLDANLSPSLLAGLAGRFRSDRRVIVEPVSV
                     EKARRHDPALHDLFLLTPTEEEVDALCPDPASFRRERRIQHLLVTRGRQGAILYDAAG
                     VREFPPNEVVETEDTTGAGDLLLAG"
BASE COUNT          499 a         1007 c         1092 g          494 t
ORIGIN      
        1 ccacgtgaac cacacggcgg agaagccacg ctacggagtc ctgatgtgcg tgaacgggac
       61 cggtatcctc tatagctgga tccgcaggct gctcttcggc gacacgggcg ccgttccgta
      121 cgaccgcctg aaccaggcgg ccgcggaggc tcccgtcggc tcggacggga tggtgatcct
      181 tccctacggg aacggcgccg agcgcacgct ggagaaccgc aaccccggtg cctcgttcct
      241 ggacgtggac ttcaaccgcc acgggatccc gcatctcctg cgctcggcgc aggaggggat
      301 cgccttcgcg ttccagtacg ggctgcgcgt catgcgggat ctcgggatcg aggtgcggac
      361 cgtcaaggcg ggggacgcga acctgttcag gagcccgctc ttccgcgagg cgttcgccac
      421 ggtgagcggc acccgggtgg agctgtacga gacggacggc gcgcaggggg ccgcccgagg
      481 agcgggagtg ggcgcgggga tctatcgctc tccgaaggag gcgttcgtcg ggctggcggc
      541 ccgaggcgcc gtcgagccgg atccctccaa ggccggcgcc tacgccgacg cgtatggccg
      601 atgggagacg gagctgaagc gggtgctgcg gtagccgaga cgcgtagggc ccctgggcgc
      661 taagccaggg ggactccggc ggagagcggg ttctccttct ttccggagcc gatgatcttc
      721 acgccgttga cgaaaacgta ttcgacgccg gacggcgcct tgtccgtttc cttgccggtg
      781 gtgttgtcct ggatgccttc ccagtccagg accacgatat ccgcggccat gccctccttg
      841 aggagacccc ggtccgtgat ggcgaaccgc tccgccgttg cgccggtcat cttgcggatg
      901 ccgtcctcca ccgagagaag ctttcgatcc cggatgagag acaggagccg ggggaacgcg
      961 gcgtaagcag cggggttctg gacggcacct cggtccaccc aggcatccgt catgaagagg
     1021 gacgcggggt ggcggaccag ctcctccagg atgcgatcgt tggagtaccg gtgacagagc
     1081 acccgggccc gaccgccgct gcgatgcgcg atgtcgatca gcgcgtccac cggggacagg
     1141 cggcgcaccc gggcgatctc gttcaggaac cggccgttgt actccgcaag ctcctcggcg
     1201 cgggtgtcgg tgacctgcac ctcggcggga ccgaacccca ggagacgctc gatcaggaag
     1261 atctggcgtt tcagcttccg cagggacgcg gtctcctcat acgcggccgc tccgcgcgcg
     1321 aggaaccacg acggaagcac cacgttgatc caggaggctc cgaagggata ggggtaggtg
     1381 tcgaacttca cgtcgacgcc ctgcgcgatg gcgagatcga tcagccgaag cgccttctcc
     1441 gcggtccgcc aggtccgtga gcccacgaag atgagatggg agatctccag gcgcacgccc
     1501 gtggaccggg ccaggtccag gatctcccgc agggcgatca ggttgtgggg ctctcccccg
     1561 agccgcaagg gatacccggg ggcaagcgcg gagtatgccc gaaggtggac gctcaggatc
     1621 ttgcccttgc gccggaccag gagcgccacc tcgcgcagct cttcgggccg ggaatagatg
     1681 cccggctcgt actgcagtcc catggagact ccacgggccc cctcggccat ggccttctcc
     1741 agaagccaca gcagctcctt gatctcgtag gggtgcagcg gagaggggtc cagccctcgg
     1801 atcgagatgc gcgtggagcc gtgtccggcc agcagcgcca ggttgtggct gatgccggag
     1861 gaggcgaggc gctcgaagta gtcggccacg gagtcccact gcagcgacag gaagccgttg
     1921 gccatcatgt tgtccgcgag ccggtctttc cacgaggagg agggacggaa cccggccgcg
     1981 gaggtcccgc agttgccggc caccacggtc gtgatcccct gggcaacgaa cggatacttc
     2041 agctcgtcgt gccctttgat gggaacgtgc cagtccaggt gggagtgcgc gtcgatgaat
     2101 cccggggcaa ccacccggcc ggtgcaatcg atggtgaggc cggtggtacg gatgggcatg
     2161 cgggagatgc ggtggatccg gccgccccgg atgagcaggt tggcgtggta ggcgcgcgcg
     2221 ccactgccgt ccaccaggag gccgtttttc aggacgatgt ccggatttct tccgtcgacg
     2281 ccgcgcgccg tcatgatcga tccactctat agcacagcgg acggagaatt gacagacgcc
     2341 ggggaaacgc gcatcgttgc cgcatgatct gggtcgtcgg agccgttgcc atcgacctca
     2401 tcgcgatgcg cgatcggttc ctcgacggaa cctccaaccc gtccgacatc cggctgggtc
     2461 tgggcggcgt gggctatcgc gtcttcagca atctggacac tccccgacgg ttcatcacgg
     2521 cgctggcggc ggatcctctc tcggcctggg cccgcaaggc gttggagagc gaagagggcg
     2581 tgctcatcca ggcggtggaa gaccgggatg ccgggcctcc gctctacctg gccttgatgg
     2641 agagcggcag cctcaaggtg gccgcctcgg acttccggat cgtggaacag cagctttcgg
     2701 aggacttcgt ggtacgcgcg ctgcaggccg gatccccgcg ggacttcctg gtgctggacg
     2761 ccaacctgtc tccatccctc ctcgccggtc ttgcggggcg gttccgctcg gatcgccggg
     2821 tgatcgtcga accggtgtcc gtcgagaagg cgcggcggca cgacccggcc ctgcacgacc
     2881 tcttcctgct cacgcccacg gaagaggagg tggacgccct ctgccccgac cccgcctcct
     2941 tccgcaggga gcggcgcatc cagcacctcc tggtcacccg gggaagacag ggcgccatcc
     3001 tctatgacgc cgccggggtg cgcgagttcc cgccgaacga ggtggtcgag acggaggaca
     3061 ccacgggggc gggggacctg ctccttgccg ga
//
LOCUS       DASRZS010000529         3354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4910822, whole genome shotgun sequence.
ACCESSION   DASRZS010000529 DASRZS010000000
VERSION     DASRZS010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4910822"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1060
                     /locus_tag="VFH83_12905"
     CDS             62..1060
                     /locus_tag="VFH83_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018495540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487318.1"
                     /translation="MGPYIVRRLLYMAVIILAVSVVGFIIIQLPPGDYVTSYIQRLAS
                     SGTSLTVSTELVESMRQSFGLDKPIYAQYGLWMWRILHGDFGYSLDWKRPVNQILAER
                     VPLTIIISLLSTLFVYLIAIPIGIYSATHQYSVLDYVFTVVGFAGIAIPNFILALVIM
                     VLALKIFGMSIGGLFSIEFMTQGWSVAKVVDMLKHLPVPIIVIGLAGTGGLIRVMRGC
                     LLDELRKQYVVTARAKGLSEQNLLFKYPVRVAVSPIISTIGWLLPGIFAGETITAIVL
                     NMPTVGPVLFQALMAKDMFLAGSIVVILTCLTVVGTFLSDVLLVLVDPRIRFTRGA"
     gene            1064..2170
                     /locus_tag="VFH83_12910"
     CDS             1064..2170
                     /locus_tag="VFH83_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487319.1"
                     /translation="MGTKEPEAYEESYYTASQWNLVARKFKKHRLAIASVVILALFYF
                     LAVFCEFFSPYLPTTRFSAYLYAPPQRVRFIDAKGFHLRPFVYAYTRSTDPNTFEKIY
                     TEDRSNPRPIRFFVHGDPYKLWGLLRTDIHFFGVESGPILLFGADNLGRDLVSRNFYG
                     SRVSLSIGLIGVSLSFLLGCILGGISGYYGGRTDTFIQRVIEFLTSIPTVPLWMALSA
                     ALPPNWSPIKVYFGITIILSVIGWCGLARVVRGKLLELREEDFVMAAKLVGLPERAII
                     SRHLLPSFVSYLIVSLTLSVPGMIMGETTLSFLGLGIRAPAVSWGTLLQDSQNVRTVL
                     LHPWLFIPVGFVVLTVLAFNFVGDGLRDAADPYK"
     gene            2189..3007
                     /locus_tag="VFH83_12915"
     CDS             2189..3007
                     /locus_tag="VFH83_12915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6487320.1"
                     /translation="MGADIKTVPLGTSFFARAETSTMAWLGMAGAVINARGTIVLIDP
                     LLTAVESSGKLLCEGDVPLRIPLPLSAADLPRVDAVLYTHADADHFGMMTLASLVQRH
                     SCRFIAPPPVLEAIRKTGVQPARLTLAREGDRVMIGDARIRVTPALHDWQEKDPWKRE
                     DCCGYLVQCADGTIWHPGDTRLIDDLYAVKGVDVFLFDVADVTTHLGPSGSAALAASC
                     GARVLVPYHYGTFDLPPGTYATCDPDAAKPFLKESKARWLQLNPGEPLTLPIGP"
     gene            complement(2967..>3354)
                     /locus_tag="VFH83_12920"
     CDS             complement(2967..>3354)
                     /locus_tag="VFH83_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6487321.1"
                     /translation="PITMITTFFTQLQKARGATESITALLDEPEETERGIGISAQDLP
                     HVFDRFFKADRSRTQANGAGGSGLGLSIVQRIVALHSGTVTAESAGLDRGSCFTVRLP
                     LSPPGASEGPAAGPTARSAASAAPQG"
BASE COUNT          538 a         1100 c         1029 g          687 t
ORIGIN      
        1 tcttcccgcc ggcgaacgct ggcgcgaaga cgcccatcct cgggaatggg acggagaacc
       61 catgggaccc tatatcgtgc gcaggcttct ctacatggcc gtcatcattc tcgccgtctc
      121 ggtcgtcggg ttcatcatca tccagctccc accgggcgac tatgtcacct cctatatcca
      181 gaggctggcc tcgtccggca cctccctgac ggtgagcacg gagctggtag agtccatgcg
      241 ccagtcgttc ggcctggaca agccgatcta cgcccagtat gggctgtgga tgtggaggat
      301 cctccacggg gacttcgggt actctctcga ctggaagagg cccgtgaacc agatccttgc
      361 cgaacgggtc cctctgacca tcatcatctc tctcttgagc accctgttcg tctatctcat
      421 cgcgataccc attggcatct actccgccac ccatcagtac tcggttctcg attatgtctt
      481 caccgtcgtg gggtttgcgg ggatcgccat tccgaacttc atcctggcac tcgtcatcat
      541 ggtgctcgcg ctcaagatct tcgggatgag catcggaggg ttgttctcga tcgagttcat
      601 gacccagggg tggagcgtag ccaaggtcgt cgacatgctg aagcacctgc ccgtcccgat
      661 cattgtgatc ggactggcgg ggaccggtgg cctgatccgg gtcatgcgcg gctgcctgct
      721 ggacgagctg cgcaagcaat acgtcgtgac ggcgcgcgcc aaggggctct cggaacagaa
      781 cctgctcttc aagtatcccg tccgggtcgc cgtgagcccg atcatcagca cgatcggatg
      841 gctcctgcct ggcatatttg ccggggagac cattacggcc attgtcctca acatgccgac
      901 ggtggggccc gtcctgttcc aggcgctcat ggccaaggac atgttcctcg ccggcagtat
      961 cgtggtgatc ctcacctgcc ttaccgtggt gggcaccttt ctctccgacg tgctcctcgt
     1021 gctcgtcgat ccgaggattc gcttcaccag aggggcctga cgcatgggga cgaaggaacc
     1081 ggaagcctac gaggagagct actacaccgc ttcccagtgg aacctggttg ccaggaagtt
     1141 caagaagcac aggctggcaa tcgccagcgt ggtgatcctc gccctgttct atttcctggc
     1201 ggtcttctgc gagttcttct caccgtatct gccaacgacc cgtttctccg cctacctcta
     1261 cgccccgcct cagcgcgttc gcttcatcga cgccaaggga ttccacctgc ggccgttcgt
     1321 gtacgcctac acgcgcagca ccgacccgaa cacgttcgag aagatctaca cggaagaccg
     1381 gtccaacccg aggccgatcc gattcttcgt ccacggcgat ccttacaagc tctgggggct
     1441 tctccgcacg gatatccact tctttggtgt cgagagcggg ccgatcctgc tctttggcgc
     1501 cgacaatctt gggcgcgacc tggtttccag gaacttctac ggctcgcggg tgagcctttc
     1561 gatcggcttg atcggcgttt cccttagttt ccttctcggg tgcatcctgg gcggcatctc
     1621 gggatactac ggcggcagga cggacacctt catccagcgg gtgatcgagt tcctgacctc
     1681 cattcccacg gtcccgctgt ggatggcgct cagcgcggcc ctgccgccga actggtctcc
     1741 catcaaggtc tacttcggga tcacgatcat cctctccgtg atcgggtggt gcggtcttgc
     1801 ccgtgtcgtg cgagggaagt tgctcgagct gcgcgaggaa gactttgtca tggccgcgaa
     1861 actggtgggt ctaccggagc gggccatcat ctctcgtcat ctgcttccct cttttgtaag
     1921 ctatctcatc gtcagcttga ccctgtcggt cccgggcatg atcatggggg agaccacgct
     1981 gagcttcctt gggctgggca tacgcgcccc cgcggtgagc tggggcacgc tgctccagga
     2041 ttcccagaac gtccgaacgg tgcttctgca tccgtggctc tttatcccgg tcgggttcgt
     2101 cgtgctgacg gtcctggcat tcaacttcgt cggggatggc ctgcgcgacg cggcggaccc
     2161 gtacaagtaa gcgcagcagg gagctggtat gggagccgac atcaaaaccg ttccgctcgg
     2221 gacctctttc ttcgcgcgcg ccgagacgtc cacgatggcc tggctcggca tggcgggagc
     2281 cgtcatcaac gcccgcggca ccatcgtgct gatcgatccc ctcctgacgg cggtggagtc
     2341 cagcggaaag ctcctgtgtg agggcgatgt cccgctgcga atcccgctgc ccctgagcgc
     2401 cgccgacctc ccaagggtgg acgccgttct gtatacccac gcggacgcgg accacttcgg
     2461 aatgatgacg cttgcctccc tggtgcagag gcactcctgc cggttcatcg ccccgccccc
     2521 cgtgctggag gccatccgca agaccggcgt ccagcccgcc cgattgaccc tggcgcggga
     2581 aggggaccgg gtgatgatcg gcgacgcccg gatccgcgtc acgccggccc tgcacgattg
     2641 gcaggagaaa gatccgtgga agcgggagga ctgctgcggt tatctggtcc agtgcgcgga
     2701 tgggaccatc tggcatccgg gagacacccg gctgatcgac gacctgtatg cggtcaaggg
     2761 cgtggacgtt ttccttttcg atgtggcgga cgtgaccacg cacctggggc cgtccggttc
     2821 ggccgctctg gcggcctcct gcggcgccag ggtactggtg ccgtaccact acggcacctt
     2881 cgacctgcct ccgggaacgt acgccacctg tgaccccgac gccgcgaagc cgtttctgaa
     2941 ggaatcgaag gcgcgctggc ttcagctcaa ccctggggag ccgctgacgc tgccgatcgg
     3001 gccgtagggc ctgccgccgg gccctctgat gcgcccggcg gcgaaagcgg gaggcgcacg
     3061 gtaaaacagg atccgcgatc cagtcccgcg ctctccgcgg tgaccgtccc ggagtgcagt
     3121 gccacgatcc gctggacgat ggagagcccg agcccactcc cgccggcgcc gtttgcctgg
     3181 gtccgggacc ggtccgcctt gaagaagcga tcgaaaacgt ggggcagatc ctgggcgctg
     3241 atcccgatac cccgctcggt ctcctcgggc tcgtcgagaa gcgccgtgat gctctccgtc
     3301 gccccgcggg ccttctggag ctgggtgaag aacgtggtga tcatcgtgat cggc
//
LOCUS       DASRZS010000530         7639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2609430, whole genome shotgun sequence.
ACCESSION   DASRZS010000530 DASRZS010000000
VERSION     DASRZS010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2609430"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="VFH83_12925"
     CDS             complement(<1..1514)
                     /locus_tag="VFH83_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487322.1"
                     /translation="MDTPQEHRPGPLSRFFALLGRARPPYAALAVALAFSVAATLVGL
                     VIPLFLKGMVDGFSLSSLGVRQIVLIALAFAGQAVGSAASGYLLTYAGQRTVANLRDL
                     LWRKQLRLSVPFFDGHASGELVSRMNNDTAVLKTLVAENVSGFITGIISVIGAVAILL
                     AMDWRMTAVMLGALPVTALVMVPLGRVMRRVSKGTMDQTARFTEVLSRVLSEIRLVKS
                     SNAEDREYRRGRGAIDALLALGLREGRVQSVISPIMSFVMMGLLVAIIGYGGVRIASG
                     AMSAGELVAFILYLIQIVMPITMITTFFTQLQKARGATESITALLDEPEETERGLPVP
                     PGRHTLEVSEVGFGYASSAPVLKGISFSLAPGTVTALVGPSGAGKTTVFSLLERFYTP
                     TAGAIRLDGAPIDRFSLLDWRRSIGYVPQESPLLSGTIQENLRYGVDRAVPRDELEQA
                     ARDANAHEFISALPNGYETDVGERGVKLSGGQRQRIAIAQALLRNPSLLLLDEAT"
     gene            complement(1581..2087)
                     /locus_tag="VFH83_12930"
     CDS             complement(1581..2087)
                     /locus_tag="VFH83_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487323.1"
                     /translation="MFVAPDALMAPLQAHSGEEAVEELLMALHNSQRIPEAPIPNARQ
                     AAGAGPVTFCGGIAITSIRIPSLDRVVVALGTCPRGVRFVSNPQDEASVVLLAAWGDA
                     SESDLPGRREALVDLTADPGFQRAILTATSAEEAAACFPSAPAGHYRTVFTRHYAPLV
                     PELVYVTD"
     gene            complement(2275..3420)
                     /locus_tag="VFH83_12935"
     CDS             complement(2275..3420)
                     /locus_tag="VFH83_12935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET6487324.1"
                     /translation="MAEQRRYSPPLESDGGWRVLTGKAEAATQGGVDLDRLALAWEYN
                     LHSKAKPKTPEELAANPQDNRIEPPARSSAVAVVRHGYLVGEWYQSADKTSRWNICSC
                     TKSLTGTAFGMLFEDSRRAALPGGKPISLDSAAYDFIPEGHPLSDPRKAKVTVRHLLS
                     MSSSINGEKSGIFGVQPAPGVGRFEMALGRGRAANGASASTLMGEPGQHWDYSDAAFA
                     HLSLIFRNASGMEIAEYLEGRLFGPIGFRGYDWAAFGGEGGTIGPHTISFSDLRTNAR
                     DMARFGYLHLAGGEWAGRRIVPAAWIQEATRTSQPMNRNYGLTWWVNTAGTLWPALPR
                     DAFAAMGYFGNKCYVVPSLDLVVVRLADGPWPWDEVPFLARVMEAIR"
     gene            complement(3453..5201)
                     /locus_tag="VFH83_12940"
     CDS             complement(3453..5201)
                     /locus_tag="VFH83_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET6487325.1"
                     /translation="MGSNPLREPEWLPGLIVYEVAPRAFTSPRGPETGTFASLTGKIP
                     YLRELGITGIWLTGHNLAHDSHFYGIWTQYATIRPDALDERLGTREELRAMIRAAHDA
                     GIKVFLDVITHGVMKDSPLVTEHPQWFKGGTWGMTDYDWFGDHEDLFRWWVDTWVRYG
                     VEDGIDGYRLDVSMYRPDLWQEIKRKAQDAGHPIVVFHELGPGKRGVVDFLQRDIRMK
                     DQRNTFDFSASYLTNMRRTLADVVANPTPSFTVDAELADGSHRTSAGDLRVRGVKRVS
                     RDVKNGRTGDTYGEDLFEMEIEPYLDPASLRNLWVSSGEHQTWMLRDNIAADYILEPV
                     PTAPGKRAPGSLRVRFPVRLCPKDTVSIQLSCHDDGWDGFPSGQNPYAARGSRFVFGY
                     GFLLTPAVPIFMSGEEFDADFRPLPRLSPGLFGGSGAGQGTWLYGSWLDWEQLEEPRH
                     REMLEDVKAIIRIRKEFARLIRPARREGRLDGFYELPVRTPAGVPVPYAYCDVDGLLM
                     VAANPSTDQDVTLKVPVPWRSLPIRKAGSYQVRDVWRGTPARSAARPELNELTVEVRR
                     DRVSKGGLSVVSISPA"
     gene            complement(5215..6102)
                     /locus_tag="VFH83_12945"
     CDS             complement(5215..6102)
                     /locus_tag="VFH83_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6487326.1"
                     /translation="MTEPRSPGRSEPRRQSEVGRRIRAVLGRIFLYLVMVFVLAISIL
                     PLVWVLVTSFKSNQEILSSALTLPKVWSFHGYVLAVQLAKLQLKFLTSMIVATCTTVG
                     SLLIYSMAAYVLARTRFRLKGVVFTLLISSILIPVNTMVQPVYEIIRRLGLYDTKAAL
                     ILVNIGFALPLCLFLLRSFLLSLPVELEEAAYIEGASFPRTFWRIIFPLAQPALTSAG
                     VLTFLGTWNELLYALLLTSSERNRVLPLAIRYFTQQFTFDYSAMFAALVLYIVPSLII
                     YIILQNQIMEGLVAGAVKE"
     gene            complement(6095..6970)
                     /locus_tag="VFH83_12950"
     CDS             complement(6095..6970)
                     /locus_tag="VFH83_12950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6487327.1"
                     /translation="MRKTNRIWILVFLAPAVIMFILVYLGPLILVTVSGFARWNGLER
                     MRFIGFRNYGEIFKSDEFRSAFFNTIKWGLLAAFVHTPFGILVALVLARKPRAWRLTR
                     AAFMAPNIIGWTALSILFLFIYMPKVGILNGLIHLVGFKNFDNNWLYNPSTAFLSVSV
                     IWLFYAAVITLITMSELFAISPSLAESARIDGASSFQIDLYINLPLIRPIIGTGIIIA
                     VTAIFKQFEIVFLTTGGGPGNKTMNISVMMVNRLFNSMEYGYSNALAMILLLMGMAFI
                     LIFQYAFRIGRSTYD"
     gene            complement(7073..>7639)
                     /locus_tag="VFH83_12955"
     CDS             complement(7073..>7639)
                     /locus_tag="VFH83_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487328.1"
                     /translation="TTSDAIGNTYALAANHFSKGDTAMIPNGPWMIDSFSDTQYSPAG
                     FADKVGYARYPGDMMISNLGLAYGDAVSMDVPADVREAGVEWLKFNATPEIIKLNMIR
                     IGSLTSKVQLSADEKATLGPVNREYVNAVEGVKQTIPWFQAMWDPITQNETVVKNLPA
                     YLYGTMTAEQYAQSLVDSAKKYQESQAK"
BASE COUNT         1401 a         2421 c         2559 g         1258 t
ORIGIN      
        1 gtcgcctcat cgagcaggag cagggacggg ttgcgcaaga gggcttgggc tatcgcgatg
       61 cgctgcctct gcccgccgga gagcttcacc cctcgctctc ctacgtccgt ttcgtagccg
      121 ttagggagcg cggagatgaa ctcgtgggcg ttcgcgtccc gcgccgcctg ctccagctcg
      181 tcgcgcggga cggctcggtc caccccgtac cgtaggttct cctgaatggt gccggagagc
      241 agcgggctct cctggggcac gtacccgatc gagcgtcgcc agtccaggag ggagaagcgg
      301 tcgatggggg cgccgtccag gcgtatggct ccggccgtcg gcgtgtagaa ccgctccagg
      361 agggagaaga ccgtggtctt gcctgctccg ctggggccga ccagcgcggt caccgtgcct
      421 ggggccaggg agaagctgat gcccttcagc acgggagcgc tcgaggcata gccgaagccc
      481 acctccgaca cctccagcgt atgccggccg ggcggcacgg gcaggccccg ctcggtctcc
      541 tcgggctcgt cgagaagcgc cgtgatgctc tccgtcgccc cgcgggcctt ctggagctgg
      601 gtgaagaacg tggtgatcat cgtgatcggc atcacgatct ggatgagata caggatgaag
      661 gcaaccagct ctccggcgga catcgcgccg gaggcgattc ggaccccgcc gtacccgatg
      721 atggccacca ggagccccat catcacgaag gacatgatcg gactgatcac cgactgtacg
      781 cggccctccc gcaggcccag agccagcagc gcgtcgatgg cgcctcttcc ccgacggtac
      841 tcccggtcct cggcgttgga ggacttcacc agtcggatct ccgaaagcac ccgagacagg
      901 acctcggtga agcgcgccgt ctggtccatg gttcccttgg agacccgccg catcacccga
      961 cccagcggaa ccatgacgag ggccgtaacc ggcagcgctc cgagcatgac cgccgtcatc
     1021 ctccagtcca tggcgaggag aatggctacc gccccgatga cggagatgat gccggtgatg
     1081 aagccagaga cgttctccgc aaccagtgtc ttcagcaccg ccgtgtcgtt gttcatgcgg
     1141 gagaccagct ctccgcttgc gtgcccgtcg aagaacggca cggagagcct gagctgcttg
     1201 cgccagagca ggtcccgaag gttggccacg gtccgctgcc ctgcatacgt gagaagatag
     1261 ccggatgccg cgctgcccac ggcctggccg gcaaacgcca gggcgatcag gacgatctgc
     1321 cggaccccga gagaggagag ggagaaccca tccaccatcc ccttcaggaa cagggggatg
     1381 acgaggccta cgagggtggc ggctacgctg aaggccagcg cgaccgccag ggccgcgtag
     1441 ggcggcctgg cgcgccccag gagcgcgaag aaacggctga gcggtccggg tcgatgctct
     1501 tgcggtgtgt ccatgcccgg aagctatcgc cctcgtgtaa acggaatgta aacgatcgag
     1561 acgggtctgg gacgccggga tcagtccgtc acgtagacga gctcaggaac cagcggcgcg
     1621 tagtgtcgcg tgaacacggt gcggtagtgt cccgccggcg cggaggggaa acaggctgcc
     1681 gcctcttccg cagaggtagc ggtgaggatg gcgcgctgga acccggggtc cgccgtgagg
     1741 tccaccaggg cttcacgacg ccccggcagg tccgattcgg acgcatcacc ccaggcggcc
     1801 agcagcacca cggatgcctc gtcctgcgga ttgctcacga accggacccc gcgggggcag
     1861 gttcccagcg caaccaccac gcggtcgaga gaagggatcc gaatggaagt gatcgcgatc
     1921 ccaccgcaga acgtgaccgg tccagcgccg gctgcctgtc gcgcgttggg aatgggagcc
     1981 tctgggatcc gctgggagtt gtggagcgcc atgagaagct cctccaccgc ctcctctccg
     2041 gagtgtgcct ggagcggcgc catgagcgca tcaggcgcca cgaacatttt gtcctttgca
     2101 tccatagacg gtatcaagca ggaatcgtgc cacgactgcg aagaaaacgt tagccacaac
     2161 cccttgccga caaacaagtt gcggatggca gatgcaggag atgccgaggc gccgagagaa
     2221 agctaccgga atgtaggagc ggcgcctcgg cgcgctaccg tctcgtaggt ttggtcatcg
     2281 aatggcttcc atcacccttg ccaggaacgg gacctcgtcc cagggccagg gtccatcggc
     2341 caggcggacc accacgagat ccagcgatgg caccacgtag cacttgttgc cgaagtatcc
     2401 cattgcggcg aacgcgtccc ggggaagcgc cggccacagc gtgccggctg tgttcaccca
     2461 ccacgtgagg ccgtagtttc ggttcatcgg ttgagaggtg cgggtggcct cctggatcca
     2521 cgccgcggga acgatccgtc ggcccgccca ctccccgccg gcaaggtgca ggtacccgaa
     2581 cctcgccatg tctctggcgt tcgtccgcag gtcgctgaag gagatcgtgt gcggcccgat
     2641 ggtgccgcct tcgccgccga acgcggccca gtcgtagcca cggaacccga tcggtccgaa
     2701 caggcggccc tccaggtact cggcgatctc catccccgag gcgttgcgga agatcaggct
     2761 gaggtgggca aaggcggcat cgctgtaatc ccagtgctgg cccggctctc ccatcagggt
     2821 ggacgcgcta gcgccgttcg ccgcccggcc ccttcccaag gccatttcga agcgccccac
     2881 ccccggtgcc ggctgcacgc cgaagatccc ggacttctcg ccgttgatgg acgagctcat
     2941 gctgagcaga tggcggaccg tgaccttcgc cttgcgcgga tcgctgagcg gatggccctc
     3001 cggaatgaaa tcataggcgg cgctgtccag gctgataggc ttgccaccag gcagcgcggc
     3061 ccggcggctg tcctcgaaga gcatcccgaa cgcggtgccg gtcaacgact tggtgcagga
     3121 gcagatgttc caacggctgg tcttgtccgc ggactggtac cattccccca ccaggtaacc
     3181 gtggcggacg acggccaccg cggagcttcg ggcgggaggc tcgatgcggt tgtcttgggg
     3241 gttggccgcc aactcttccg gcgtcttggg cttggccttc gaatggaggt tgtactccca
     3301 ggccagggcg agccggtcga ggtccacgcc gccctgggtg gcggcctccg ccttgcccgt
     3361 caggacgcgc cagcctccgt cggattcgag aggtggggaa taccgtcgct gctcggccat
     3421 gttcgtacct cctgccgacg tagcgtcggc gatcaggcgg ggctgatgga tacaaccgag
     3481 agcccaccct tgctgacccg gtccctgcgg acctccacgg tgagctcatt cagctcgggg
     3541 cgggcagccg aacgagccgg ggtgcctctc cacacgtccc gcacctggta ggacccggct
     3601 ttgcgaatgg ggagagacct ccacgggaca ggaaccttga gggtcacgtc ctgatccgta
     3661 gaagggtttg cggctaccat cagaagcccg tcgacgtcgc aataggcgta gggcacggga
     3721 accccggccg gagtgcgaac cggcagctcg tagaaaccat ccagccggcc ttcccgtcgt
     3781 gccgggcgga tcaagcgcgc aaactctttt cggatgcgga tgatcgcttt cacgtcttcc
     3841 agcatctctc gatgccgggg ttcctccaac tgctcccagt ccagccagct tccatagagc
     3901 caggtcccct gccctgctcc gctgccgccg aagaggcccg ggctcaaacg cgggagcggc
     3961 cggaagtccg catcgaactc ctcgcccgac atgaagatcg gcacggcggg cgtaagaagg
     4021 aaaccgtagc cgaacacgaa ccggctgcct cgggccgcgt agggattctg ccccgagggg
     4081 aacccgtccc aaccgtcgtc atgacagctc aactggatgg agaccgtgtc cttgggacag
     4141 agccggacgg ggaagcgcac gcgcaggctg ccgggcgctc gcttgcccgg cgcggtcggc
     4201 accggctcca ggatgtagtc agcggcaatg ttgtcccgca gcatccaggt ctgatgctcc
     4261 ccgctggaga cccagaggtt gcgcagactc gccgggtcca ggtacggctc gatctccatc
     4321 tcgaagaggt cttcgccgta cgtatccccg gtgcgcccat tcttcacgtc ccgggacacg
     4381 cgcttcacgc cccgcacccg cagatcgccg gcagaggtcc gatgagagcc gtcggccagc
     4441 tccgcgtcca ccgtgaagga aggcgtgggg ttggccacca cgtccgccag ggttcggcgc
     4501 atgttcgtga ggtaggacgc ggagaagtcg aaggtgttgc gttgatcctt cattcggatg
     4561 tcgcgctgga ggaagtccac cacgccgcgc ttccccgggc caagctcgtg gaacacgacg
     4621 atgggatgac ccgcgtcctg cgccttgcgc ttgatctcct gccagaggtc cggccggtac
     4681 atcgacacgt cgagccggta gccgtcgatc ccatcctcca ccccgtagcg cacccaggtg
     4741 tccacccacc agcggaagag gtcctcgtgg tctccgaacc agtcgtagtc cgtcatgccc
     4801 cacgtcccgc ccttgaacca ctggggatgc tccgtcacca gcgggctgtc cttcatcacg
     4861 ccgtgggtga tcacgtccag gaagaccttg atcccggcgt cgtgcgccgc ccggatcatg
     4921 gcgcggagct cctcacgggt ccccaaccgc tcatccagcg cgtccggccg gatcgtggcg
     4981 tactgggtcc agatcccgta gaagtgggag tcgtgggcaa ggttgtgtcc ggtgagccag
     5041 atcccggtga tccccagctc acggaggtag gggatcttcc cggtgaggct ggcgaacgtc
     5101 ccggtctcgg gcccccgggg agacgtaaag gcccgggggg ccacctcgta gacgatcaga
     5161 ccggggagcc actccggctc ccgaagggga ttgctgccca tgcgaggtcc ctcctcattc
     5221 cttgaccgcg cctgccacga gcccttccat gatctggttc tggaggatga tatagatgat
     5281 caaggagggg acgatgtaca gcaccagcgc ggcgaacatc gcggagtagt cgaacgtgaa
     5341 ctgctgggtg aaataccgga tggccagggg aagcacgcgg ttcctctccg aagaggtcag
     5401 gagcagcgcg tagaggagct cgttccaggt gcccaggaac gtgagcaccc cggcgctggt
     5461 gagcgccggc tgcgccaggg ggaagatgat ccgccagaag gtccggggaa agctggcccc
     5521 ttcgatgtat gcggcctcct ccagctctac gggcagactg agcaggaagc tgcgcagcag
     5581 gaacaggcat agcggaagag cgaacccgat gttcaccagg atcagggcgg ccttggtgtc
     5641 atagaggcca agacggcgaa tgatctcgta gaccggctgc accatggtgt tcacggggat
     5701 caggatcgaa gagatgagga gggtgaacac cacgcccttg agccggaacc gggtgcgggc
     5761 cagcacatag gcggccatcg agtagatgag cagggagccc acggtggtgc aggttgccac
     5821 gatcatcgag gtcagaaact tcagctggag cttggcgagc tgcaccgcca ggacatagcc
     5881 gtggaaggac cagaccttgg gcagcgtgag ggcggaggaa aggatctcct ggttgctctt
     5941 gaacgaggtg acgagcaccc acaccagggg caggatcgag atggccaaga cgaacaccat
     6001 gaccaggtag aggaagatcc gcccgagcac cgcgcggatc cggcggccca cctcggattg
     6061 ccgccgcggc tccgagcggc ccggcgaacg cggctcagtc atacgtgctc ctgccgatgc
     6121 ggaacgcgta ctggaagatg aggatgaacg ccatccccat gagcagcagg atcatggcca
     6181 gggcgttgga gtacccgtac tccatgctgt tgaagagccg gttcaccatc atcaccgaga
     6241 tgttcatggt cttgttcccc ggcccgccgc cggtggtgag gaacacgatc tcgaactgct
     6301 tgaagatggc ggtgacggcg atgatgatcc ccgtcccgat gatgggccgg atcaggggga
     6361 ggttgatgta caggtcgatc tgaaagctcg acgccccatc gatccgggcg gattcggcca
     6421 gggaagggga gatcgcgaag agctcggaca tcgtgatcag ggtgatgacg gccgcgtaga
     6481 acagccagat aaccgagacg gacaggaacg cggtggacgg gttgtacagc cagttgttgt
     6541 cgaagttctt gaacccgacc aggtggatca gcccgttgag gatcccgacc ttgggcatgt
     6601 agatgaagag gaagaggatg gagagcgcgg tccagccaat aatgttcggc gccatgaagg
     6661 ctgcccgggt gagccgccac gctcgaggct ttcgggccag caccagtgcc accaggatcc
     6721 cgaagggcgt gtggacgaac gcggccagga gcccccactt gatggtattg aagaacgcgg
     6781 agcggaactc gtcgctcttg aagatctccc cgtagttgcg aaacccgatg aaccgcatgc
     6841 gctccagccc gttccaccgg gcaaagccgc tcacggtcac caggatgagg gggcccaggt
     6901 agacgaggat gaacatgatc acggccgggg ccaggaacac caggatccag attctgttgg
     6961 ttttcctcac gatcttctcc gagggatcgg gacgggcgat cccggcggtg ccgtctggcg
     7021 gtcgccgagg aggggacgcg ggcggattcc gccgcccgcg tccagggaaa cgctacttgg
     7081 cctgggactc ctggtacttc tttgcggagt ccaccagcga ctgagcatac tgctcggcgg
     7141 tcatggtgcc atagaggtac gccggcaggt tcttcacgac ggtctcgttc tgggtgatgg
     7201 ggtcccacat ggcctggaac caggggatgg tctgcttcac gccttccacc gcgttgacgt
     7261 actcgcggtt gacgggaccc agggtggcct tctcgtcggc cgagagctgg accttggagg
     7321 tgaggcttcc gatgcgaatc atgttcagct tgatgatctc gggcgtggcg ttgaacttca
     7381 gccactccac gccggcctcc cgaacgtccg cgggcacgtc catggacacc gcgtcaccgt
     7441 acgccagacc gaggttggag atcatcatgt ctccgggata gcgcgcgtag ccgaccttgt
     7501 ccgcgaaccc ggcgggggag tactgcgtgt cgctgaagct gtcgatcatc cagggtccgt
     7561 tggggatcat ggcggtatca cccttggaga agtggttggc cgccagcgcg tacgtgttcc
     7621 cgatggcatc cgaggtggt
//
LOCUS       DASRZS010000531         3855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5299067, whole genome shotgun sequence.
ACCESSION   DASRZS010000531 DASRZS010000000
VERSION     DASRZS010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5299067"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VFH83_12960"
     CDS             <1..532
                     /locus_tag="VFH83_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6487329.1"
                     /translation="AGDAPYLVVANHSSLYDIPALMGAVPGVAIMGREYLTRIPGFGR
                     LLTALHYIPVDTESPRKAHASLERAAEVARNGVSVGMFPEGTRTPDGQVQSLRRGFVQ
                     VLRKSGLDLVPARITGTFALKPKGQLTMDPREQIHVRLGAPVRNADLTGLSDREILRR
                     VHTLLQGGEGGPDASL"
     gene            519..1448
                     /locus_tag="VFH83_12965"
     CDS             519..1448
                     /locus_tag="VFH83_12965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET6487330.1"
                     /translation="MLRSDGWYFIVNPVAGDGYGTACAEIVREQMRRHGAEGEIAFTR
                     AKGHATELAAEFAREGAPIIVGVGGDGTLSEVAQALVGNPQVVFGGVAAGTGNDFIHI
                     LGFPDRFEDPQWDALFEGKQAAMDVGRCNGRHFINGMGLGFDAQVAYENYHMEDGGEV
                     RGGKKSKYLWHIVKNLLAYQEKPMRVTIDGKTEERRSFLNTIANGRRLAGGMYVTPKA
                     MANDGKLDICMTDPLSLPMRFRELISVTRQTHLANPEVHYVQTGSALFEFDAEVPAHL
                     DGELIFARRFEVDVLPGALRAIIDPAGQPYFLK"
     gene            complement(1433..2296)
                     /locus_tag="VFH83_12970"
     CDS             complement(1433..2296)
                     /locus_tag="VFH83_12970"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6487331.1"
                     /translation="MTQTTTPTATAAAEGTATDGRKVLTQEQVNGFWKNGFLRFGKIL
                     DDELLETMRREYDREFELVSTGRSDRSMFRNLSGASTDDLDTINRSEKKMLQIMQMCE
                     RNLHFRKLVYNDAILDVIEDLMGPNIQLFHDQALYKPAHHGGPVHWHQDNAYWRCSPA
                     TLISCWLTLDDVDEQNGAMQLIPGTHTSLLDHAKAKQSDLLLDITEQIDASKAVVVDL
                     PAGGAMFHHCQTFHYTAPNTTDRQRRAFAIHFMQPGTRSLRQQGYLGVSWGNPMLRMG
                     LGNSGSGYFKK"
     gene            complement(2374..>3855)
                     /locus_tag="VFH83_12975"
     CDS             complement(2374..>3855)
                     /locus_tag="VFH83_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487332.1"
                     /translation="LSFSYFDRTPVGWIMARVTSDSGRIAGLITWGLIDVCWALSNIT
                     SSTVFMFLIDWRLAALVFISIPFLVIVAVQFKNRIVVQFRKVRKLNSKITAAYNENIM
                     GARVVKALCRERRNLEEFSSLTGEMRAAGFRAAWLSALFLPVVQIISALAIGVIVWVG
                     GMQAWAAAISVGGIQAFLGYVTFMMWPIQDLARVYADMQQSIASAERSFSLIDAVPEI
                     VDRPTALQTDADPGSIAARVEFDRVDFHYKEGVPVLKDFSLAVEPGETIALVGPTGGG
                     KTTIVNLLCRFYEPTGGRIRIGGTDYLDLPLRAIQSRIGMVLQTPHLFSGTVRENIRY
                     GRLSATDAEVEEAANLAGAHSFIQRLDKGYDSEVGEGGNRLSVGQKQLVSLARAVLAR
                     PELFVMDEATSSVDTLTESLIQRGMERLMEGRTSFIIAHRLSTIKRADRILVIEGGRI
                     SEMGTHAELIRARGHYYGLYTRQFRHEHEQAQEALQILEGDAL"
BASE COUNT          631 a         1279 c         1304 g          641 t
ORIGIN      
        1 ggccggtgac gctccctacc tggtagtggc caaccactcc agcctgtacg atatcccggc
       61 gctgatgggc gccgtgccgg gggtcgccat catggggcgg gagtacctga cccgcatccc
      121 cggcttcggc cgccttctga cggcgctgca ctacatcccg gtggacacgg agagcccccg
      181 caaggcgcac gcctccctgg agcgcgccgc ggaggtagcc cggaacggag tgtccgtcgg
      241 catgttcccc gaagggaccc ggacgccgga cggccaggtg caatccctgc ggcgcggatt
      301 cgttcaggtc ctccgcaaga gcggattgga cctggtcccg gcgcggatca ccgggacctt
      361 cgcgctgaag cccaagggac agctcacgat ggaccccagg gagcagatcc acgttcgcct
      421 gggagctccg gtccgcaacg ccgatctgac cgggctgtcg gatcgagaga tcctgcggcg
      481 agtgcacacg ctgctccagg gcggcgaagg gggaccggat gcttcgctct gatggttggt
      541 acttcatcgt caacccggtc gccggcgacg ggtatggcac ggcgtgcgcc gagatcgtgc
      601 gcgagcagat gcgccgccac ggcgccgaag gggagatcgc cttcacccgg gcaaagggac
      661 acgccaccga gctggcggcg gagttcgccc gggagggcgc gcccatcatc gttggggtcg
      721 gcggcgacgg gaccctgagc gaggtggcgc aggcgctggt aggcaacccg caggtggtct
      781 tcggcggcgt ggcggcgggg accgggaacg acttcatcca catcctgggg ttccccgacc
      841 ggttcgagga tccccagtgg gatgcgctct tcgagggcaa acaggcggcc atggacgtag
      901 ggcgctgcaa cggccgccac ttcatcaacg gcatgggtct gggcttcgac gcccaggttg
      961 cgtacgagaa ctaccacatg gaagacgggg gcgaggtgcg gggagggaag aagtccaagt
     1021 acctctggca catcgtcaag aacctcctgg cctaccagga gaaacccatg cgggtcacca
     1081 tcgacgggaa gaccgaggag cggcggagct ttctcaatac catcgccaac ggccgccgcc
     1141 tggccggtgg gatgtacgtc acgcccaagg ccatggccaa cgacggcaag ctggacatct
     1201 gcatgacgga cccgttgagc cttccgatgc gattccgcga gctgatctcc gtgacccgcc
     1261 agacccacct ggccaacccg gaggtccact acgtccagac cggctccgcc ctcttcgagt
     1321 tcgacgccga ggtgccggct cacctcgacg gagagctgat cttcgcgcgg cggttcgagg
     1381 tggacgtgct gccgggcgcc ctcagggcca tcatcgaccc ggcgggacag ccctacttct
     1441 tgaagtagcc gctccccgag ttccccagac ccatcctcag catcgggttt ccccaggaga
     1501 cgcccaggta gccctgctgt cggagcgacc gcgtccccgg ctgcatgaag tggatggcaa
     1561 aggcacggcg ctggcgatcc gtcgtgttcg gcgcggtgta gtggaacgtc tggcagtggt
     1621 ggaacatggc cccgcccgcg gggagatcca ccacgacggc cttggacgcg tcgatctgct
     1681 ccgtgatgtc cagcaggagg tcgctctgct tggcctttgc gtggtccaac agggaggtat
     1741 gggtccccgg gatgagctgc atggccccgt tctgctcgtc cacgtcgtcg agggtcagcc
     1801 agcagcttat cagggtcgcc gggctgcagc gccagtaggc gttgtcctgg tgccagtgga
     1861 ccgggccgcc gtggtgcgcc ggcttgtaca acgcctggtc gtggaagagc tggatgttcg
     1921 gccccatcag atcctcgatg acgtccagga tggcgtcgtt gtagacgagc ttgcggaagt
     1981 ggaggttccg ctcgcacatc tgcatgatct ggagcatctt cttctcggac cggttgatgg
     2041 tatccaggtc gtccgtggag gcgcccgaga ggttgcggaa catggaccgg tccgagcggc
     2101 ccgtgctgac cagctcgaac tcgcggtcat actcccggcg catcgtctcc aggagctcgt
     2161 cgtccaggat ctttccgaac cggaggaacc cgttcttcca gaagccgttc acctgctcct
     2221 gggtgaggac cttgcgcccg tccgtcgcgg tcccttccgc ggcggcggtc gctgtcggag
     2281 tcgtggtctg cgtcatgaga gaacggtact gacggaagac ctccggcgac aaccgccacg
     2341 cggttggggc ccgcgccggg cccgtctcgc ggcctacagc gcgtccccct ccaggatctg
     2401 aagcgcctcc tgcgcctgct cgtgctcgtg acggaactgg cgcgtgtaca ggccgtagta
     2461 gtggccgcgc gcccggatga gctccgcgtg cgtgcccatc tcgctgatcc gcccgccctc
     2521 gatcaccagg atccggtcgg cccgtttgat ggtagagagg cggtgggcga tgatgaagct
     2581 ggttcgcccc tccatgagcc gctccatgcc ccgctggatc aggctttcgg tgagggtatc
     2641 caccgagctg gtggcctcgt ccatcacgaa gagctcgggc cgcgccagca ccgcgcgcgc
     2701 caggctgacg agctgcttct gccccacgga gaggcggttg ccgccctccc ctacctcgga
     2761 gtcatagcct ttgtccaggc gctggatgaa cgagtgcgcc cccgcgagat tcgccgcttc
     2821 ctccacctcg gcgtccgttg cggacagccg accgtagcgg atgttctccc gcaccgtccc
     2881 cgagaacagg tggggcgtct ggaggaccat cccgatccgg gactggatgg cgcgcagggg
     2941 caggtccagg tagtcggtcc cgccgatgcg gatccgtccg ccggttggct cgtagaatcg
     3001 gcacaggagg ttcacgatcg tggtcttgcc tcctcccgtg ggtcccacca gggcgatggt
     3061 ctctccaggc tccaccgcca gactgaagtc cttcaggacg ggcacgccct ccttgtagtg
     3121 gaagtccaca cggtcgaact ccacccgggc ggcgatgctg ccggggtcgg cgtccgtctg
     3181 gagcgccgtg gggcggtcca cgatctcggg caccgcgtcg atcagggaga agctgcgctc
     3241 cgcggaggcg atggactgct gcatgtcggc gtagacgcgc gccaggtcct gaatcggcca
     3301 catcatgaac gtcacgtagc ccaggaaggc ctggatgccg ccgacgctga tggccgccgc
     3361 ccacgcctgc atcccgccga cccagacgat caccccgatg gccagcgcgc tgatgatctg
     3421 caccacgggg aggaagagcg ctgagagcca ggcggcccgg aaccccgcgg cccgcatctc
     3481 cccggtcagg ctggagaact cctccaggtt tcgccgctcc cgacagagcg ccttgaccac
     3541 gcgcgcgccc atgatgttct cgttgtacgc cgcggtgatc ttggagttca gctttctcac
     3601 tttgcggaac tggaccacga tccggttctt gaactggacg gcgacgatca ccaggaacgg
     3661 gatggagatg aacaccagcg cggccagcct ccagtcgatg aggaacatga agaccgtgga
     3721 ggaggtgatg ttggacagcg cccagcagac gtcgatcagt ccccaggtga tcagccccgc
     3781 gatcctgccg gagtccgagg tgacccgcgc catgatccag cccaccggcg ttcggtcgaa
     3841 gtaggagaag gacag
//
LOCUS       DASRZS010000532         4103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5301068, whole genome shotgun sequence.
ACCESSION   DASRZS010000532 DASRZS010000000
VERSION     DASRZS010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5301068"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VFH83_12980"
     CDS             complement(<1..787)
                     /locus_tag="VFH83_12980"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6487333.1"
                     /translation="MIEVPIEYAAPGDILGKYHTFRKYEMGMSSTVDLERGYRLTERV
                     IRKLRDEYHVQYIFIEEPSPELKDVSFIEPYSEATRQQGVSTIIHAMKRVKTTKVLDL
                     RVFGAVVEDILQNVFNILQNGKGSFKVLSNTFMRVQSHDFYTWEHSVNTAVYAAIIAL
                     SIPRVFDSMGLTYSIGRTSRLENLVMNMLLHDVGKMKIPEAVLNKPCRLDESELALVK
                     KHPQYGVAFIREVNDELESAGLPWIPSYYMQACLSHHQAFDGSG"
     gene            954..1664
                     /locus_tag="VFH83_12985"
     CDS             954..1664
                     /locus_tag="VFH83_12985"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6487334.1"
                     /translation="MTEGESGPGAADRSVAGEVLIISPRKLDSELLAYALEREICPNC
                     RIVPDVSSLKDALRPGQGGDGKATGNAAKTLLLIDCVENDFDRVMRELSDHQHDPSDT
                     LVVALYNIYPGWGIEEEALRFGIKGFFYRHDDFKLLTKGLHAILGREVWVSREILLRS
                     ATGVQRKKRSAIQEKTGLSIREIEILLLLSSGLQNEEIAQKLFISPNTVKTHLYNIFK
                     KIDVPNRLQATLWVAKNI"
     gene            1843..2304
                     /locus_tag="VFH83_12990"
     CDS             1843..2304
                     /locus_tag="VFH83_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HET6487335.1"
                     /translation="MDDKAVEKQPQRTADSNPACRAIPLALVLTAVLAAFSCALYNPA
                     SFVKNKSDPKAIPVTAPQVTLQWDPPAGSTPASYIVSFRIHGTTSWTALATIPAATQP
                     SYVVPYSAVGAGSFDFGVVAVSSSGSDSPEHTSLDATADPTTGWYLNWWGP"
     gene            2444..3478
                     /locus_tag="VFH83_12995"
     CDS             2444..3478
                     /locus_tag="VFH83_12995"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6487336.1"
                     /translation="MRARAKPVLKRPWLHHVIIICYIVAPLVNILGVKLFVGAPFGVI
                     FARLFEGYGVLATIWLITAPIVGVSLYFVQRFSWYVFLGHSAIILADFVYKWTAHPAF
                     YLRTVPGVHNILLLAGNIALVAGVGYVIQKDFRAPYFQVLNRTWRERVRIPIYHPVTI
                     DGTERIMSDLSDGGCFVTWDCPECAAGHRVALSFRSQSLNIECQGEIMRITRKGIGIR
                     FIRLPLEKKRDIRRLIRNRYSLRHKVDIPCVSRFGDEETAARMLDVSPTGCYVQAQYA
                     GLHAGSEGVLHVAIGEARPAVNLPGNVVWINQGDVGSRPAGFGFHFGRRQASFVKEAA
                     ERFGMGILVR"
     gene            complement(3509..>4103)
                     /locus_tag="VFH83_13000"
     CDS             complement(3509..>4103)
                     /locus_tag="VFH83_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET6487337.1"
                     /translation="EFERLGSNRTIKVDVRIITATNKDLEAEIAGGRFREDLYYRLNV
                     VTISLPPLREREGDVELLSEYFLGKFNRVLNKKVRRISPPALRLLNGYRWPGNIRELE
                     NTMERAVLMAETDQITPEDLSLPFKPEHKASEAIIRIPADGLKWEEVEKDLIVQALSM
                     RGWVQKEAAQLLGLSTRVLNYKIKQFGITHPSWKANR"
BASE COUNT          761 a         1271 c         1278 g          793 t
ORIGIN      
        1 agccgctccc gtcgaatgcc tggtgatgcg aaaggcacgc ctgcatgtaa tatgaaggga
       61 tccacggcag ccccgcgctt tccagctcgt cgtttacctc gcgaatgaag gccaccccgt
      121 actgggggtg cttctttacg agggcgagct cggattcgtc caaccggcaa ggcttgttga
      181 ggacggcttc cgggatcttc atcttgccga catcgtgcag cagcatgttc atgacgaggt
      241 tctccagccg ggacgtgcgc ccgatggagt acgtgaggcc catggagtcg aacacacgcg
      301 ggattgaaag cgcaatgatg gccgcgtaga ccgccgtgtt gaccgagtgt tcccacgtgt
      361 agaaatcgtg agactgcacg cgcatgaacg tgttggagag aaccttgaag cttcccttgc
      421 cgttctgaag gatgttgaac acgttttgca ggatgtcctc caccacggcg ccgaagacgc
      481 ggaggtccag caccttcgtg gttttcacgc gcttcatggc gtggatgatg gtggagacgc
      541 cctgctgacg cgtggcctcg ctgtagggct cgatgaagct cacgtccttg agctcgggag
      601 acggctcctc gatgaagata tactgcacgt ggtactcgtc ccgcagcttg cggatgacac
      661 gctccgtgag ccggtagccg cgctccaggt ccacggtgga gctcatgccc atctcgtact
      721 tgcggaaggt gtggtatttt ccgaggatgt cgccgggcgc tgcgtactcg atcgggactt
      781 cgatcattcg gcactttcct attggcggcc agcggtcgcg aagctatagc ggcgcagtat
      841 atcacgttgg cgccgcttgg caagtgagat atctccggcc gggaccctgg gctgccgtac
      901 ggggggtgcg gttgaaggtt ggcaagaacc atgctaaagt gcacgaatta caaatgactg
      961 aaggagaaag tggtcccggc gccgccgacc gaagcgtggc cggcgaagtc ctcattatca
     1021 gtccccgcaa gctggacagc gagctcctcg cgtatgccct ggaaagggag atctgtccga
     1081 actgccgtat cgtgccggac gtctcatccc tcaaagacgc gctgaggcca ggtcaggggg
     1141 gagacgggaa ggcaaccgga aacgcggcca agacgctcct gctgatcgac tgcgtggaaa
     1201 acgacttcga ccgcgtcatg cgggagctct ccgaccacca gcacgaccct tcggataccc
     1261 tggtggtagc cctgtacaac atctaccccg ggtggggaat agaagaagaa gcgctcaggt
     1321 tcggcatcaa ggggttcttc taccgtcacg atgacttcaa gctcctgacc aagggtctgc
     1381 acgccatcct gggacgggag gtgtgggtct cgcgcgagat actcctgcgg agcgcgaccg
     1441 gcgtgcagcg gaagaaacga tccgccatcc aggaaaagac cggcctctcc atccgggaga
     1501 tcgagatcct tctcctgctt tccagcggcc ttcagaatga agagatcgcg cagaagctgt
     1561 tcatcagtcc gaacaccgtc aagacgcacc tctacaatat cttcaaaaag atagatgtgc
     1621 ccaatcggct gcaagccacc ctgtgggtgg ccaagaatat ctgaactgcg acagcggtga
     1681 gcttgagttt caattgtgta atatggggat cacgatgcgg cgggtgaact gatttcgatt
     1741 gactattcct caggccggca agaagttagg cacacggacc ggtggaaggt gttcggcatg
     1801 gttgttgcat ccatcccggt gagaacaggg aggacagaca ccatggatga caaagcagtt
     1861 gaaaaacagc cgcagcggac ggcggattca aatcccgcct gtcgcgcaat ccccctcgcc
     1921 ctcgtcctga ccgccgttct tgctgcgttc tcctgcgcgc tttacaaccc cgcaagcttc
     1981 gtcaagaaca agtccgaccc gaaggccatt cccgtcacgg cgccccaggt cacgctgcag
     2041 tgggatccgc ccgcaggcag taccccggcc agctacatcg tgagcttccg catccacgga
     2101 accacgtcgt ggaccgccct agcgacgatt ccggccgcca cacagccctc ctatgtcgtg
     2161 ccctactcgg cggtcggtgc ggggagcttc gacttcggag tggtagcggt gagcagctcg
     2221 ggctccgact cgcccgagca cacttccctg gatgccactg ccgacccgac tacgggctgg
     2281 tatctcaact ggtgggggcc ctgagcaccg ggcgtggtcc gggcgccggg ccggctccgc
     2341 cggcccccgc cgccccggcg cgaaagcccg cccttgaggg gagctcttcc cgcgcgtaga
     2401 atagccggat agaaaacatc gtccattcaa aaggaagtga ccgatgcgcg cccgagcgaa
     2461 acccgtcctg aagagaccct ggctgcacca cgtcatcatc atctgctaca tcgtcgcgcc
     2521 gcttgtgaat atcctcgggg tgaagctgtt cgtcggcgcg ccgttcggtg tcatattcgc
     2581 caggctgttc gaaggttacg gggtgctcgc caccatctgg ctgatcaccg ccccgatcgt
     2641 cggggtgtcc ctgtacttcg tccagcggtt cagttggtac gtgttccttg gccacagcgc
     2701 aatcatcctc gcggatttcg tctacaagtg gactgcccac ccggcgttct acctcaggac
     2761 ggtgccgggc gtgcacaata tcctgctgct ggcgggcaac atcgcgctgg tcgcgggtgt
     2821 cggctacgtc atccagaagg acttccgcgc gccctacttc caggtcctga atcgcacgtg
     2881 gagagagcgc gtgcgcatcc cgatctacca cccggtcacc attgacggta ccgagcgcat
     2941 catgagcgac ctctcggacg gcggctgctt cgtgacctgg gactgccccg aatgcgcggc
     3001 gggacaccgc gttgcacttt ccttcaggag ccagtccctg aacatcgagt gccaggggga
     3061 aatcatgcgg atcacgcgca aggggatcgg catccgcttc atccggctgc ccctggagaa
     3121 gaagcgcgac atccgccgcc tgatcaggaa ccgctactcc ctgcggcaca aggtggacat
     3181 tccctgtgtg agccgcttcg gagacgagga aaccgcggca cggatgctcg acgtctcgcc
     3241 gactggctgc tatgtccagg cgcaatacgc ggggctccac gcgggctccg agggcgtgct
     3301 gcacgtggcc atcggggaag cgcggccggc ggtcaacctt cccgggaatg tggtgtggat
     3361 caaccaggga gacgtcggtt cgaggccggc cgggttcggc ttccatttcg gccgtcgcca
     3421 ggcaagcttc gtgaaggagg cggcggagag attcgggatg gggatcctgg tccgctgaca
     3481 ccccgggcgg ggcggtccgg ccccgatgct accggttcgc cttccagctc gggtgcgtga
     3541 tgccgaactg cttgatcttg tagttcagaa cgcgcgtgga aagccccagg agctgtgccg
     3601 cttccttctg gacccagcca cgcatggaga gggcctgcac gatcaggtcc ttctccactt
     3661 cctcccattt caggccgtcc gcggggatgc ggatgatcgc ctcgctggcc ttgtgctcgg
     3721 gcttgaacgg gaggctcaag tcctccggcg tgatctggtc ggtctctgcc atgagcaccg
     3781 cccgctccat ggtgttctcc agctcccgga tgttgcccgg ccagcggtag ccgttgagaa
     3841 ggcgcagggc cggtggcgag attcggcgga ccttcttgtt aagcacgcgg ttgaacttgc
     3901 ccaggaagta ctccgagagg agctccacgt ccccttcccg ctcccgaagc ggtgggagcg
     3961 agatggtcac cacgttcagt cgatagtata gatcctcgcg gaagcggccc ccggcgatct
     4021 ccgcctccag gtccttgttg gtggccgtga tgatgcgcac gtccaccttg atcgtccggt
     4081 tgctccccag cctctcgaac tcg
//
LOCUS       DASRZS010000533         3095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3364639, whole genome shotgun sequence.
ACCESSION   DASRZS010000533 DASRZS010000000
VERSION     DASRZS010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3364639"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1271)
                     /locus_tag="VFH83_13005"
     CDS             complement(87..1271)
                     /locus_tag="VFH83_13005"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6487338.1"
                     /translation="MHTSVSPPLGRAGSRGANPRPVRITLCITLRCNLACSYCYVEKR
                     PERMSEETARKAVAFAFEHTPPDRPLEFGFFGGEPLLEIGLLEEITHMAERHPRYDPM
                     RVTFSVTTNGTIFSDRIAAFLRRHGVKTCISCDGPPEIQNLHRRGAAGEPTAALVEET
                     TREALRALPRVLVNAVYGPATLDALPRTVEYLSGLGLRQIHLNADYSADWACIDPSEI
                     ISTYRRTAQRYLDWYRAGIPHYINLIDEKIAVALRGGYLAQERCQMGTGELAITPDGG
                     IYPCERLIGTRNNGPHRIGCLEHGIDPFRMDCRRRADGDRNRECETCPVRDYCMNWCG
                     CSNAFMTGFYNRVGSFLCASEKAAIQVAIETLRTMEAVLGPVFLHHYAGQPSFNSMSA
                     GG"
     gene            complement(1425..2588)
                     /locus_tag="VFH83_13010"
     CDS             complement(1425..2588)
                     /locus_tag="VFH83_13010"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6487339.1"
                     /translation="MSRLERLTPLLESFVKNGPPGCACKVERAGKVLYERYVGLSDVA
                     ARRPVTPDLIYRFYSNSKPITCVAALVLYERGAYQMNDPLSEYLPEFRDVRVYQTGPN
                     GTLTAEPAKRSVRIRDLFTMTSGLCYPGDDVETARQVAVAQREMESRLGPAGYDVRTM
                     SRTLAGIPLAFEPGTHWRYGLSHDVLGALIEVLSGKSLGRFLEEEIFTPLGMKDTSFR
                     LPKEKAGRLCPMYDRSAEGALTRNDSWDAPYQPDARFESGGGGLLSTVADYGRFGQML
                     AHDGALQGTRILGGRTVQLMRTNHLNAEVLKDYNWEHMAGYGYGLGVRVMIDPAAQGS
                     NGTIGEFGWGGLAGTWLLADAKEELSVVYAQQTMPNQEPIHQTLLRNVVYGGL"
     gene            complement(2758..>3095)
                     /locus_tag="VFH83_13015"
     CDS             complement(2758..>3095)
                     /locus_tag="VFH83_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487340.1"
                     /translation="DPRNTNPGRYCCGKCTVGLWRHLLAGGLDRQEERLRLGIRHLHE
                     MRDGEGQWRVYPFWYAVLALSEADLPGAVTELRYAAKRLEETARRPGQASLYAQRRHE
                     LARRALERL"
BASE COUNT          542 a         1026 c          986 g          541 t
ORIGIN      
        1 acgttactcc ttccaggatt tccatgctga tccatgaaac gacaagcctt gggattccgg
       61 ggggcccgcg aatgaagtgc atcgcctcaa cctcccgcgc tcatgctgtt gaaggacggc
      121 tggccggcgt aatggtgaag gaataccgga ccaaggacgg cctccatcgt cctgagcgtc
      181 tcgatggcaa cctggatggc agccttctcg gacgcgcaga ggaaggagcc gacccggttg
      241 tagaatccgg tcatgaacgc attcgagcac ccacaccagt tcatgcaata gtcgcgaacc
      301 gggcaggtct cacattcccg gttgcgatcg ccatccgccc tgcgtcggca gtccattcga
      361 aacggatcga tcccgtgttc caggcagccg atccgatgcg gtccgttgtt ccgggtgcct
      421 atgaggcgct cgcagggata gatgccgccg tccggcgtga ttgccagctc ccccgtaccc
      481 atctggcacc gttcctgggc caggtacccc ccgcgaagcg caacagcgat cttctcgtcg
      541 atcagattga tgtagtgagg aatacccgcc cgataccaat ccaggtaccg ctgcgcggtt
      601 cgccggtacg tcgagatgat ctcactcgga tcgatgcagg cccagtccgc cgagtagtcc
      661 gcattcaggt ggatctgtcg caaacccagg cccgacaggt attccacggt acggggcagg
      721 gcatcgagcg tggccggccc atagaccgcg ttgaccagga cccgagggag cgcgcggagc
      781 gcctcccgtg tcgtctcctc cacgagggcg gccgttggtt cacccgccgc cccgcgtcga
      841 tggaggttct ggatctccgg aggcccatcg cagctgatac aggtcttgac gccatgacgc
      901 cgcaggaacg cggcgattcg gtccgagaag atggtcccgt tggtcgtcac ggagaaggta
      961 acccgcatag gatcgtagcg gggatgccgt tccgccatgt gggttatctc ctcgagcaga
     1021 ccgatctcca gcaagggctc tccgccaaag aagccaaact ccaaggggcg atccggcggc
     1081 gtgtgctcaa aggcgaacgc gaccgccttg cgcgccgttt cctcggacat ccgttctggg
     1141 cgcttttcca catagcagta cgagcaggcc aggttacagc gaagggtgat gcaaagagtg
     1201 atgcgaacag gacgcgggtt cgcgccgcga ctccccgcac ggccgagcgg gggtgacacg
     1261 ctggtgtgca tgtccgaacg agcccgcctg cagaaagcag ggcaaggccc cctcacattt
     1321 cgcggtatga tagcacaccc ggcccgggag cgcaagcgct cgcgcagcgg gagcagcggg
     1381 aaccgagggg gctcagcggc cgacgctctt gaagcctgcg gctctcagag ccccccgtag
     1441 accacgtttc gcaggagcgt ctggtggatc ggctcctggt tgggcattgt ctgctgggcg
     1501 tagaccacgg aaagctcctc cttggcgtct gcgagaagcc aggtaccggc aagcccgccc
     1561 cacccgaact caccgatggt gccgttggaa ccctgggccg cgggatcgat catgacccga
     1621 accccgagcc catacccgta tcctgccatg tgttcccagt tgtagtcctt gaggacctcg
     1681 gcgttgaggt ggttggtccg catgagttgg accgtcctgc cgcccaggat ccgcgttccc
     1741 tggagcgccc cgtcatgggc caacatctgg ccgaaccgtc cgtaatccgc caccgtcgag
     1801 agaagccctc ctccgcccga ctcgaagcgc gcgtcaggct gatagggcgc gtcccaggag
     1861 tcgttgcgcg tcagggcgcc ttccgcggac cggtcgtaca ttgggcacag cctgcctgcc
     1921 ttctccttcg gaaggcggaa agaggtgtcc ttcatcccca acggagtgaa gatctcctct
     1981 tccaggaaac ggcccaggct ctttccagag agcacctcga tcagggcgcc caggacgtcg
     2041 tggctcagac cgtacctcca gtgcgtgccc ggttcgaagg ccagagggat gccagcgagc
     2101 gtccgggaca tcgtccgcac gtcgtagccg gcggggccga ggcgggactc catctcgcgc
     2161 tgggcgaccg ccacctggcg cgcggtctcg acatcgtctc ccggatagca caagccggag
     2221 gtcattgtga acaggtctct gatgcggacg gaacgcttgg cgggctcggc ggtgagcgtg
     2281 ccgttcggcc cggtctggta gacccgcacg tccctgaact caggaaggta ttccgacagg
     2341 ggatcgttca tctgatacgc gccccgctcg tacaacacga gcgccgcaac gcaggtgatt
     2401 ggcttcgagt tggagtagaa ccggtagatc agatccgggg tgaccggtcg gcgcgccgcg
     2461 acgtcggaca ggccgacgta ccgttcgtag agcaccttgc ctgcccgttc taccttgcag
     2521 gcgcagcccg gtggcccgtt cttcacgaag ctctcgagca acggcgtcag gcgttccaga
     2581 cggctcatag gctcctccct cgattccagc cgacccgggg ctcctcgtgg tgctcacgcc
     2641 cccggcagat cctccggcag attataagga agggaacgat tccgatccag tgcgcctggg
     2701 gccaaccgct cctgagcgct gccggaacgt ccttttcgat ccccgcggtc gatggcccta
     2761 cagccgttcc agggcgcgcc gcgcgagctc gtgtcggcgc tgagcgtaaa gcgacgcctg
     2821 gcccgggcgg cgcgccgtct cctcgagcct ctttgcggcg taccgaagct cggtcacggc
     2881 gcccgggagg tctgcctcgg acagggcgag cacggcgtac cagaagggat agacccgcca
     2941 ctgcccctct ccgtccctca tctcgtggag gtggcggatc ccgagacgga gccgctcctc
     3001 ctgtcggtcg aggccgccgg caagcaggtg cctccacaag ccgacggtgc acttcccgca
     3061 gcagtatctg cccgggttgg tattccgtgg gtcat
//
LOCUS       DASRZS010000534         2743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1838032, whole genome shotgun sequence.
ACCESSION   DASRZS010000534 DASRZS010000000
VERSION     DASRZS010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1838032"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1668)
                     /locus_tag="VFH83_13020"
     CDS             complement(184..1668)
                     /locus_tag="VFH83_13020"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6487341.1"
                     /translation="MLSPHELPRSDPESQGVDSAAIAAFLDAADTAVKDLHSFMVLRH
                     GVVIAEGWWRPYRPDVPHMLFSLSKSFTSTAAGLAVSDGLLSVEDRVLSFFPEDAPRK
                     VSDNLARMRVRDLLTMTTGHAEEATRRTFARPDRRAEKAFLSTPVEYAPGTHFVYNTA
                     ATYMVSAIVQKRTGKTLVQYLTPRLFEPLGIREPIWDSYANGVNFGGFGLHLRTEDIA
                     RFGQLYLNRGLWNGKRLLSESWVEEATARQVPNGNTGNPDWIQGYGYQFWRCQPRGVY
                     RGDGAFGQYCIVMPEQDAVVAITSGVSDMQAVLTALWKTLLPALGAEPTIGAQMAAGA
                     GAQPLETRLQSLRRDPPQGPSTSPRASGVDGVTYRFEANAQKLTSVRFRFCSESCAVT
                     VVRGRHRDTVEAGYGEWRSGSLALPYETTGVISDRPVVASCRWVEADALELTVRQTGA
                     PFVYTWRCRFGDRELLLDSTVNVAFDPTPAPTLRALAPSPVRSN"
     gene            complement(1729..2139)
                     /locus_tag="VFH83_13025"
     CDS             complement(1729..2139)
                     /locus_tag="VFH83_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET6487342.1"
                     /translation="MPSERMLDVGRLILRLTLAGLILFHGIDKVLHGIAWMSGPLRSA
                     GLPQVVGYGVFLGEVVAPLFVILGLGTRVAALVIVANMVMALVLDAGRFILTINSVGG
                     WGVETEAFYLLSAVTLFFVGGGGISVTRGKGILS"
     gene            complement(2206..2694)
                     /locus_tag="VFH83_13030"
     CDS             complement(2206..2694)
                     /locus_tag="VFH83_13030"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6487343.1"
                     /translation="MRRSISLGTVLFLLMAGALFASGSSGGSASSGSSSGGSGSTTTA
                     LDWYNTGYAAANAGKYGDAITDFNNAIALKADYAEAYNMLGYCTRKMGDVAKAFTYYD
                     KALKLKPNFPEAREYYGEAYLQQGNLAKAVQQYIILVKGNNRSNAAELLDQIDAYVNK
                     NA"
BASE COUNT          500 a          891 c          903 g          449 t
ORIGIN      
        1 cgtccttgtt cgccttccag accaccatag gcggcatgac cagacggttg ggaaggcgca
       61 gcaccccggt agaccaggag ctggcaagcg tggggtaggg cacgaagtac ctccggaagg
      121 gggaatcgta gtcgaacctg cccccttgga caagcgggcg ccgcgctcaa ccgacggctc
      181 gggtcaattc gaacggaccg gactcggggc gagcgctctc agcgtcggcg ccggtgtagg
      241 gtcaaaagcg acgttgaccg tcgagtccag gagcagctcc cggtcgccga accggcaccg
      301 ccacgtatac acaaagggcg cgccggtctg gcggacggtc agctccaggg cgtccgcctc
      361 cacccaccgg cagctcgcca ccacgggccg gtccgagatc actcccgtgg tctcgtaggg
      421 cagggccagc gaaccagagc gccactcccc gtagccggcc tcgaccgtgt cgcgatgtct
      481 gccccggacg acggtcacgg cgcaggattc ggagcagaag cggaaccgga ccgaagtgag
      541 tttctgggcg ttggcctcga accggtacgt caccccgtcg acacccgagg cgcgcggcga
      601 ggtcgagggc ccctggggcg gatccctgcg aagggactgg agcctggtct ccaggggctg
      661 ggcgcccgct ccggcagcca tctgggcgcc aatggtcggc tccgcgccga gagccgggag
      721 gagagtcttc cagagggccg taagcacggc ctgcatgtca ctcacccctg aggtgatggc
      781 gaccacggca tcctgctcgg gcatcacgat gcagtactgg ccgaacgcgc catcaccacg
      841 gtagacccca cgcggttggc atcgccagaa ctgataaccg tacccctgga tccaatcggg
      901 gttcccggtg ttgccgttgg gtacctgacg ggccgtggcc tcctcgaccc acgactcgga
      961 gaggagacgc ttcccgttcc agagaccccg gttcaggtag agctggccga accgggcgat
     1021 atcctcggtc cgaaggtgaa gaccgaaccc gccgaaattg acgccattgg cgtagctgtc
     1081 ccagataggc tcccggattc ccaggggctc gaagagccgc ggggtaaggt actgcaccag
     1141 ggtctttccg gttcgcttct gcacgatggc tgacaccatg tacgtggcgg ccgtgttgta
     1201 gacgaagtga gtgcccggcg cgtactcgac cggtgtcgag aggaacgcct tctcagcccg
     1261 ccgatccgga cgagcgaacg ttcggcgcgt agcctcctcg gcgtgtcccg tggtcatggt
     1321 caggaggtcg cggacccgca tccgggccag gttgtcgctc acttttctgg gcgcatcttc
     1381 ggggaaaaag gacaggaccc gatcttccac gctcagaagg ccatccgaga cggccaatcc
     1441 ggccgccgtg gatgtgaagc tcttgctcaa ggagaaaagc atgtgaggga cgtccggacg
     1501 ataggggcgc caccagcctt ccgcgatcac cacgccatgg cgcagcacca tgaagctgtg
     1561 cagatctttg acggcggtgt ccgcggcgtc cagaaaggcg gctatcgcgg ccgaatcgac
     1621 tccctgagac tcggggtctg acctgggaag ctcatgggga ctcagcatgg tcggcaagaa
     1681 tagaggccgc gccccatccg ggtcaaggag agcggccgag ggtccgcctc agctcagaat
     1741 ccctttccct cgcgtgacac tgataccgcc gccgcccacg aagaagaggg taacggctga
     1801 gaggaggtag aacgcctccg tctccacgcc ccatccgcca accgagttga tcgtcaggat
     1861 gaatcgaccg gcgtccagca ccagggccat gaccatgttt gcgacgatga ccagggccgc
     1921 cacgcgagtc cccaggccca ggatcacgaa gagcggcgct accacctccc ccaggaacac
     1981 tccgtagccg acgacctgcg gaagcccggc ggagcgaagc ggaccgctca tccaggcgat
     2041 cccgtggagg accttgtcga ttccatggaa gaggatgagc ccggccagcg tcaggcgaag
     2101 gatgagtctg ccaacatcca gcattcgttc ggacggcacg gcatctctcc aaagcaagcc
     2161 ggtccccgcg gcccaggtgg gtggcgggtg accggcaggg cagggtcagg cgttcttgtt
     2221 cacgtaagca tcgatctggt cgagaagctc cgcggcgttg ctccggttgt tgcccttgac
     2281 caggatgatg tactgctgga ccgccttggc aaggttcccc tgctgcaggt atgcctcgcc
     2341 gtagtactcc cgagcctcgg ggaagttcgg cttcagcttc aacgccttgt cgtagtaggt
     2401 gaatgccttg gccacgtcac ccatcttccg ggtgcagtac cccagcatgt tgtacgcttc
     2461 ggcgtaatcc gccttcaacg cgatggcgtt gttgaagtcg gtgatggcgt caccgtactt
     2521 tccggcgttg gcggccgcgt accccgtgtt gtaccagtcc agggccgtgg tcgtgctgcc
     2581 gcttccgccg ctggaagagc cgctgctggc cgagccgccg ctgcttcccg acgcgaaaag
     2641 cgcgccggcc atgagcagga agagaaccgt cccaagcgaa atgcttcgtc tcacgtcgta
     2701 acctcctggg gcttgacgcc cgcgcggggg cggtgagggg tcc
//
LOCUS       DASRZS010000535         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2210840, whole genome shotgun sequence.
ACCESSION   DASRZS010000535 DASRZS010000000
VERSION     DASRZS010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210840"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VFH83_13035"
     CDS             complement(<1..630)
                     /locus_tag="VFH83_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020619326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6487344.1"
                     /translation="MKRATSYHLMLAPSVLFVLVFSYGPMVGIVIAFQKFNPVQGFFH
                     SPWLGLDNFRYVALLPDAFQVLWNTIFIAFMKIVIGFAVPIVVALLLNETRSAGFKRT
                     IQTVVYLPHFLSWVILSGIIIDILSPSSGIVNTLLKATGRPPIFFLGNEHWFPWVLIF
                     SDVWKEFGFGTVIFLAAIAGVNPVLYEAAVIDGATRMQQLLHVTLPGIVS"
     gene            complement(643..1776)
                     /locus_tag="VFH83_13040"
     CDS             complement(643..1776)
                     /locus_tag="VFH83_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6487345.1"
                     /translation="MSDLTNRERFLRVMEYRPVDRVPNWEAGVWGQTIDRWKAEGLNP
                     YQLSWDWFTGEDSLGYDPREFIPVDFGMMPAFEPEVLEETDRYVVARNSLGVVTRALK
                     EGTAHGTRASMDQYLRFPVEGQEDFRELKKRFEASLTGRYPVGWRELMLPRWKSRQHV
                     LVLGRNCSTLGFYWRAREWMGTENVSYAWYDQVPLMHEMMEFIADFTMEVARPIVEAT
                     DIDYVFLNEDMAMKSGPLLSPDTYRTFILPHMRRLVDYLKTHGVRYVLVDSDGNTEPL
                     IPLLLEAGVDGLWPLERASWDTDPAFLRRKYGKGLRLWGAVDKREIAKGPRAIDEHLR
                     AMVPLIEEGGFIPTVDHTVPPDISLEDFRYYLKRKADLLRGCF"
     gene            complement(1852..>2474)
                     /locus_tag="VFH83_13045"
     CDS             complement(1852..>2474)
                     /locus_tag="VFH83_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487346.1"
                     /translation="ITYGSTGTIYVINRRCKNPEAAVKMLNLYIDKDLALSSDFDDSY
                     HKKDLVGGGQIPRYEYAFEQCWHPRQNLFIHQGVADYFSGKMTDKSKMVFWVKDNVQQ
                     AEDYLSGKNNGYWSTYMWSGPIGAEGVVDSYWKANLVTVSEYLGPSTETMVAKNATLN
                     KMKLETFTKIIMGASPITDFDKFVDDWKKLGGDQITKEVNDWVASK"
BASE COUNT          444 a          777 c          789 g          464 t
ORIGIN      
        1 gctcacgatc cccggcagcg tcacgtgcag gagctgctgc atgcgcgtgg ctccgtcgat
       61 gaccgcggcc tcgtagagca cggggttcac ccccgcgatg gccgccagga agatgaccgt
      121 cccgaacccg aactccttcc agacgtcgga gaaaatcagg acccacggaa accagtgctc
      181 gttccccaga aagaagatgg gcggtcgccc cgtggccttg agcagggtgt tcacgatgcc
      241 gctggaagga gagaggatgt cgatgatgat gccgctcagg atgacccacg agaggaagtg
      301 cgggaggtag accacggtct ggatggtccg cttgaaccct gcggaccggg tctcgttgag
      361 gagcagcgcc acgacgatgg gaacggcgaa gccaatgacg atcttcatga acgcgatgaa
      421 gatcgtgttc cacagcacct ggaaggcgtc cggcaggagc gccacgtagc ggaagttgtc
      481 cagaccgagc cacggagagt ggaagaaccc ctggacggga ttgaacttct ggaacgcgat
      541 gacgatcccc accatcggtc catagctgaa caccagcacg aagaggaccg acggggccag
      601 catcagatgg taggacgtgg ctcgtttcac gtcggcctcg ccctaaaagc agccccgtag
      661 gaggtctgcc ttccgtttca ggtagtatcg gaagtcttcc agggagatgt ccggcggcac
      721 cgtgtgatcc accgtcggga tgaacccccc ctcctcgatg agagggacca tggcgcgcag
      781 gtgctcgtcg atggcgcgcg gacccttggc gatctcccgc ttgtccaccg cgccccacag
      841 gcggaggccc ttcccgtact tgcgtcgaag gaacgcgggg tccgtgtccc aggacgctcg
      901 ttccaggggc cacaggccat ccactccggc ttccagcaga agcgggatca gcggctcggt
      961 attcccgtcg ctgtccacca ggacgtagcg cacgccgtgc gtcttgaggt aatccacgag
     1021 gcgacgcata tggggaagga tgaacgtccg gtaggtgtcc gggctgagga gcggcccgga
     1081 cttcatggcc atgtcctcgt tcaggaacac gtaatcgatg tccgtggcct ctacgatggg
     1141 ccgcgccacc tccatcgtga aatccgcgat gaactccatc atctcgtgca tgaggggcac
     1201 ctggtcgtac caggcgtagc tcacgttctc ggtacccatc cactcccggg cacgccagta
     1261 gaagcccagc gtggagcagt tccgtcccag gaccaggacg tgctggcggc tcttccaccg
     1321 ggggagcatg agctcccgcc agccaaccgg gtaccgcccc gtcagggagg cctcaaagcg
     1381 tttcttcagc tcccggaagt cctcctggcc ttccaccggg aagcggaggt actggtccat
     1441 gctggcgcgc gtgccgtggg cggtgccctc tttcagcgcg cgggtcacca cgcccaggga
     1501 gttgcgagcc accacgtagc ggtccgtttc ctccagtacc tccggctcga aggccggcat
     1561 catgccgaag tccaccggga tgaactctcg cgggtcgtac cccagggagt cctcaccggt
     1621 gaaccagtcc caggagagct ggtacgggtt cagaccttcc gccttccatc ggtcgatggt
     1681 ctggccccag acgccggctt cccagttggg cacgcggtcc acgggccggt attccataac
     1741 ccgcaggaac cgctcgcggt tggtgagatc gctcatgggg gctccctctg cgaagcgcgc
     1801 cctcggggcg gcgtagagcc gccccgaacg acgcgcgaca ggttcaacgg gtcacttgga
     1861 ggcgacccag tcgttgactt ccttggtgat ctggtctccg cccagcttct tccagtcgtc
     1921 cacgaacttg tcgaaatccg tgatcgggga agcgcccatg atgatcttgg tgaaggtctc
     1981 cagcttcatc ttgttgagcg tggcgttctt ggcgaccatg gtctccgtgg aggggcccag
     2041 gtattcgctc accgttacca ggttggcctt ccagtaggaa tccaccacgc cttcggctcc
     2101 gatggggccg gaccacatgt aggtggacca gtacccgttg ttcttgccgc tgaggtagtc
     2161 ctccgcctgt tggacgttgt ccttgaccca gaagaccatc ttcgacttgt ccgtcatctt
     2221 tccgctgaag tagtcggcca caccctggtg gatgaagagg ttctggcggg ggtgccagca
     2281 ctgctcgaag gcgtactcgt accgggggat ctggccgccg cccaccaggt ccttcttgtg
     2341 gtaggagtcg tcgaagtccg agctcagggc gaggtccttg tcgatgtaga ggttcagcat
     2401 cttcaccgcg gcctcggggt tcttgcacct gcggttgatc acgtagatgg tccccgtgga
     2461 gccgtaggtg atgg
//
LOCUS       DASRZS010000536         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2210960, whole genome shotgun sequence.
ACCESSION   DASRZS010000536 DASRZS010000000
VERSION     DASRZS010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210960"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..2987
                     /gene="acnA"
                     /locus_tag="VFH83_13050"
     CDS             312..2987
                     /gene="acnA"
                     /locus_tag="VFH83_13050"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HET6487347.1"
                     /translation="MKRSADPFGTLSTLKTKTATVGYYSLPRLEAQGIAPVSRMPFAM
                     KILMESVLRQINGFDVREEDVHALARWDSTESLKREFPFKPARVLMQDFTGVPAIVDL
                     AAMRDAMSRLGGDPQRINPVVPVDLVIDHSVQVDLYASSRALQMNAALEFKRNGERYQ
                     FLKWGAKAFQGFRVVPPATGIVHQVNLEFLAHVVQVRSGEGTGEIAFPDTLVGTDSHT
                     PMINGIGVLGWGVGGIEAEAALLGQPLYMLLPQIIGMRITGALREGITATDLVLTITQ
                     ALRKHGVVDRIVEFFGSGLDSLSVADRATISNMSPEYGATASYFPVDAKTLEYLRFTG
                     RSPELLDLVESYCRAQGIFRDSQSQSPQYASMLELDLGTVEPSLAGPKRPQDRVPLSK
                     AKTSWKDVGVPESNGHPTAVEVGGVHETIGHGAVVIAAITSCTNTSNPAVMVAAGLLA
                     QKAVHLGLSVPAHVKTSFAPGSKVVTQYLDSAGLTPYLEALGFHLVGYGCTTCIGNSG
                     PLNPAVSAVVKENSLVVASVLSGNRNFEGRIHPEVKANYLASPPLVVAYALAGTMDID
                     LTSEPIGRDRNENPVYLKDLWPTQAEIERAVGASLNPRMFQTVYSNVFSGDESWQAVV
                     TPTDRLYRWEPQSTYIREPTFFGGLTLKAAEIPKLSGARVLLVLGDSVTTDHISPAGA
                     IPPNGPAGKWLQEHGVSVADFNTYGSRRGNHEVMIRGTFANIRIRNRLVPGVEGGYTR
                     HLPSGEKLSVYDAATRYAAEGVPLVVIAGKEYGSGSSRDWAAKGTILLGVRAVIAESY
                     ERIHRSNLVGMGVLPLQFLPAENAASLGLTGEETLDIQTVSAPGQTVEVIAHGATERR
                     FKALTRIDSAVEYEYYKNGGILPTVLRGFLKTAST"
     gene            2996..>3915
                     /locus_tag="VFH83_13055"
     CDS             2996..>3915
                     /locus_tag="VFH83_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET6487348.1"
                     /translation="MFEPVPTNPDFPALERQTLEWWRKTDAFERLRRMRAGGPTWSFT
                     DGPITANNPMGVHHAWGRTYKDLFQRHRAMTGHDLRWQNGFDCQGLWVEVNVERDLGF
                     KTKKDIEEHGLAAFVVLCKQRVLEYAAMQTEQSVRLGMWMDWNDPDQLRLLKEKLGDP
                     AAGALTVQGPQGPVTGTAEQLIGQLGMPQMGGSYFTLSDKNNYDIWGFLKTCYENGWI
                     YKGTDVMPWCWRCGTGISQHEIVTEGYIEKKDPSLTVRFPLIDQGPGAGSLLVWSTTP
                     WTLTSNVAAAVGPELTYLKVRQGDEILYLS"
BASE COUNT          713 a         1279 c         1302 g          621 t
ORIGIN      
        1 agcgggcgcg gcatcttcac gctccccaga ccgatgagga tggactgggc ctccaggggc
       61 gcaatgaaga tgggaacggc aacgtcgaga cccatcggcc ggacgagcac cgcgttgccc
      121 tgctctgctt ttgccacggt ccagacctct gcaggcacca gcatgtctca gttctctcgc
      181 ctccaatgat agagcaattg cccggggttg gcaatacaag gcggtccaca gacggacgcc
      241 cgcgcctccg ggaggccggc cactgggttc acgggaaccc gtcatgtata ttaggacgag
      301 gagtaaactc aatgaaacgg tccgccgatc cgttcggaac cctttccacg ctcaagacga
      361 agaccgccac cgtggggtac tacagcctgc ctcgactgga agcccagggc atcgccccgg
      421 tgtcccgcat gccgttcgcc atgaagatcc tcatggaatc ggtgctccgc cagatcaacg
      481 gcttcgatgt tcgggaggag gacgttcatg ccctggcccg gtgggactcg acggagagcc
      541 tgaaacgcga gttccccttc aagccggcgc gggtcctgat gcaggacttc acgggcgtcc
      601 cggcgatcgt ggacctcgcc gcaatgcggg atgcaatgtc ccgcctgggc ggcgatcctc
      661 agcggatcaa cccggtggtg ccggtggacc tcgtcatcga ccactccgtc caggtggacc
      721 tgtatgcctc ctcccgcgcg ctgcagatga acgccgcgtt ggagttcaag agaaacgggg
      781 agcgctacca gttcctcaag tggggggcaa aggcattcca ggggttccgc gtggtgccgc
      841 cggccacggg gatcgtccac caggtgaacc tcgagttcct ggcccacgtg gtgcaggtcc
      901 gatccggtga ggggaccggc gagatcgcgt tcccggatac gctcgttggc accgactccc
      961 atacgcccat gatcaacggc attggcgtcc tgggatgggg agtcgggggg atcgaggcgg
     1021 aagccgctct gttgggacag ccgctgtaca tgctgctccc ccagatcatc gggatgcgga
     1081 tcaccggggc cctgcgggag gggatcaccg ccacggacct ggtcctgacc atcacccagg
     1141 cgctccgcaa gcacggggtc gtggacagga tcgtggagtt cttcgggtcc ggcctggact
     1201 cgttgagcgt cgcggaccgg gcaaccatca gcaacatgtc tccagagtac ggcgccaccg
     1261 ccagttactt cccggtggat gccaagaccc tggagtacct gcgcttcacc ggcaggagcc
     1321 ctgagctgct ggacctggtg gagagctact gccgggccca ggggatcttc cgggactccc
     1381 agtcccagag cccccagtac gccagcatgc tggagctgga cctgggaacg gtggagccgt
     1441 ccctggccgg gcccaagcgt ccccaggacc gcgtgccgct ctccaaggca aagacctcgt
     1501 ggaaggacgt gggggtgccg gagtcgaacg gtcatccgac ggccgtagag gtgggcgggg
     1561 tccatgagac catcgggcat ggagccgtgg tgatcgccgc catcacgagc tgcaccaaca
     1621 cgtccaaccc cgcggtcatg gttgcggccg gcctcctggc gcagaaggcg gtgcacctgg
     1681 gcctgagcgt gccggcccat gtgaagacca gcttcgcgcc cggctccaag gtggtgaccc
     1741 agtacctgga cagcgccggg cttaccccat acctggaggc gctcggcttc cacctggtgg
     1801 gatacgggtg caccacctgc atcggcaact ccggccccct caaccccgcg gtgagcgcgg
     1861 tggtgaagga gaacagcctc gtagtggcct ccgtgctgtc gggcaaccgg aacttcgagg
     1921 gcaggatcca tcccgaggtc aaggccaact acctggcctc ccctcccctg gtggtggctt
     1981 acgcgctcgc cgggaccatg gacatcgacc tcacctcgga gccgatcggt cgggaccgga
     2041 acgagaaccc cgtgtacctg aaggacctct ggcccacgca ggcggagatc gagagagcgg
     2101 tgggcgccag cctcaacccg cggatgttcc agaccgtcta ctccaacgtg ttcagcggag
     2161 acgagagctg gcaggcggtg gtgaccccga cggatcgcct ctaccggtgg gagccccagt
     2221 ccacctacat ccgggagccg accttcttcg gcgggctcac cctgaaagcg gcggagatcc
     2281 cgaagctttc cggggcgcgg gtgctcctgg tgctgggaga ctccgtcacc acggatcaca
     2341 tctcgccggc aggcgccatc ccgccgaacg gcccggcggg gaagtggctc caggagcacg
     2401 gcgtcagcgt ggcggacttc aacacgtacg gttcccgacg cggcaaccac gaggtcatga
     2461 ttcgggggac cttcgccaac atccgcatcc gcaaccggct ggtccctggg gtcgaaggag
     2521 ggtatactcg acacctgccg tcgggtgaga agctctccgt gtacgacgcc gcgacgcggt
     2581 acgccgccga gggcgtgccc ctggtggtga tcgccggcaa ggaatacgga tccgggagct
     2641 cgcgcgattg ggccgccaag gggaccatcc tcctgggcgt ccgcgccgtg atcgccgaaa
     2701 gctacgagcg catccatcga tccaacctcg tggggatggg cgtgcttccg ctgcagttcc
     2761 tcccggctga gaacgccgcc agcctgggcc tgaccggaga ggagacgctg gacatccaga
     2821 ccgtctccgc gcccggacag acggtggagg tgattgcaca cggcgccacg gagagacggt
     2881 tcaaggcgct gacgcgcatc gactcggcgg tggagtacga gtactacaag aacggcggga
     2941 tcctgccgac cgttctgcga gggtttctca agactgcatc gacttaggag gccagatgtt
     3001 cgagcccgtt ccgacaaacc ctgacttccc cgcgctggag cgccagacgc tcgagtggtg
     3061 gaggaagacc gatgccttcg aacggcttcg ccggatgcgg gcgggcggcc ctacctggtc
     3121 cttcacggat ggaccgatca cggccaacaa ccccatgggc gtgcaccacg cctggggacg
     3181 gacgtacaag gacctcttcc agcgtcaccg cgccatgacg gggcatgacc ttcggtggca
     3241 gaacggtttc gattgccagg gcctgtgggt ggaggtgaac gtcgagcggg acctcgggtt
     3301 caagaccaag aaggacatcg aggagcacgg cctcgccgcc ttcgttgtgc tctgcaagca
     3361 gcgcgtgctg gagtacgcgg ccatgcagac ggagcagtcg gtccgcctgg ggatgtggat
     3421 ggactggaac gacccggacc agctccgcct cctcaaggag aagctgggag atccagcggc
     3481 gggcgccctg acggtgcagg gcccgcaagg ccccgtcacc gggaccgcgg agcagttgat
     3541 cggtcagctc ggcatgccgc agatgggcgg ctcctacttc accctgagcg acaagaacaa
     3601 ctacgacatc tgggggtttc tcaagacctg ctacgagaac ggctggatct acaaggggac
     3661 cgatgtcatg ccctggtgct ggcggtgcgg aaccgggatc agccagcatg agatcgtcac
     3721 ggaagggtac atcgagaaga aggacccgag cctcacggtc cgcttccccc tgatcgacca
     3781 gggccccggc gccgggtccc tcctggtgtg gtccaccacg ccctggaccc tcacctccaa
     3841 cgttgccgca gcggtcgggc cggagctcac ctacctcaag gttcgccagg gagacgagat
     3901 cctctatctc tccaa
//
LOCUS       DASRZS010000537         2720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3766546, whole genome shotgun sequence.
ACCESSION   DASRZS010000537 DASRZS010000000
VERSION     DASRZS010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3766546"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VFH83_13060"
     CDS             <1..924
                     /locus_tag="VFH83_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487349.1"
                     /translation="GPITACIDVSARAPGCPSIRRIISLHAGVRRIDCAVRILKNADP
                     LGEASVAFPFDARAPRFRYEGVLGSIRPGEDTLPGASLNTVAVQNWLSLSDAQRTILW
                     SCAECPIVSLGDLWPSRVSPAHTCALPATPAPAVTNAVALGRGWVFSRILANNFGTNF
                     SVSQTGDFLFRYAVTSADAPVTDTGASWFGWEAVTPLSCITTSGPAEGPYPPCGGLAE
                     VDSQDVEILASKLAEEGGGLVVRLWNHAGQDRAVQLTLSGHRLAGAEITSMVEEGTGE
                     ALPVEGSVLRLVVPASQIVSVRLRMAAGRSR"
     gene            complement(884..2023)
                     /locus_tag="VFH83_13065"
     CDS             complement(884..2023)
                     /locus_tag="VFH83_13065"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6487350.1"
                     /translation="MAATFERLFEGDRISKRERVGLTLRHQPVDRAALHDQLSYSPSV
                     IEMYTGRRIQGFSYGPEEIGQAIRKTLDMCFPLVPPIGTDRVTDPDGFVSQRDNWTSW
                     HVSRPFDTVDGALAWVRRLIERERERSRLFDADRERDAHRALYRETQKLIGETVLCAF
                     SMTGFCGVFDRMGLELYSFFTLEHPTVLSLLMEIATDNEVRRVRAVADPALSPLILLP
                     EDFSTKQGPIFSPEFLHRFHYPYVRRLVDAWHEAGIKVLYHSDGNYKKAIPDLMACGV
                     DGFYCLEPGCGMDIVELKLRWPHMVWAGGVDGVDTMERGTPADVRRAVHHHIQATDAL
                     RTGGMFVASSSEINPPIRAENYRAMVEAAGALVNDSFRLPSAAAH"
     gene            complement(2026..>2720)
                     /locus_tag="VFH83_13070"
     CDS             complement(2026..>2720)
                     /locus_tag="VFH83_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487351.1"
                     /translation="EALARYVEAGGSLLSEACPGRLDEHAFATRGELSPTARKLFGVR
                     QRCLAVVREPGAGERWLPRERTWGEYAEHRALVGAGAFQGCAIEPSLYQETFECDGSQ
                     AIFLSGDLPAAVERSAGRGRAILLGTVVGHRGTAYRSSAAGAFVDRLLSRCGIQPQRA
                     GNVLIRKRVREGQEAWLLFNPGREESCADLDATSFRSVADLFGGELRKDGDRLTVAVP
                     ALDYRVLILATA"
BASE COUNT          440 a          953 c          878 g          449 t
ORIGIN      
        1 ggtccgatca ctgcctgcat cgacgtatcc gcccgcgccc ccggatgccc gagcatccgc
       61 cggatcatca gcctccacgc cggggtgcgc cggatcgact gcgcggttcg gatcctgaag
      121 aacgcagatc ccctcgggga ggcctcggtc gcttttccct tcgacgctcg ggcgccccgg
      181 ttccgctatg aaggggtgtt gggctccatc cgaccgggcg aagacaccct gccgggggcg
      241 tcgctgaaca cggttgccgt tcagaactgg ctctccctct cggacgccca gcgcaccatc
      301 ctttggtcct gcgccgagtg cccgatcgtg agcctcggcg acctctggcc cagccgggtc
      361 tctcccgccc acacgtgtgc gcttcccgcc accccggcgc cggccgttac aaacgccgtc
      421 gccttggggc gcgggtgggt tttctcccgg atcctcgcga acaacttcgg cacgaacttc
      481 tccgtttcgc agactggcga cttcctcttc cgctatgccg tgacgagcgc ggacgccccg
      541 gtcacggaca cgggggcctc ctggttcggc tgggaggctg tgacgcctct ctcctgcatc
      601 accacgagcg gacctgccga gggtccctat ccgccctgcg gcggccttgc cgaagtggac
      661 tcgcaggacg tcgagatcct cgcgtcaaag ctcgcggaag aagggggagg gctcgtcgtg
      721 cggctgtgga accacgcagg ccaggatcgg gcggttcagc tcacgctaag cggccatcgg
      781 ttggctggag ccgagatcac gagcatggtc gaggaaggaa ccggagaggc gcttccggtc
      841 gaaggctccg tgctacggct tgtcgtcccc gcgtcgcaga tcgtcagtgt gcggctgcgg
      901 atggcagccg gaaggagtcg ttgaccagcg ctcctgccgc ctcgaccatc gcccgatagt
      961 tctccgcccg gatgggtggg ttgatctccg aggagctggc cacgaacatg ccaccggtgc
     1021 gcagcgcatc cgttgcctgg atgtggtggt gcaccgctct acgaacatcc gcgggagtgc
     1081 cgcgctccat ggtatccacg ccgtccaccc cgccggccca caccatgtgg ggccaccgca
     1141 gcttgagctc cacgatgtcc attccacagc caggctccag gcaatagaag ccgtccacgc
     1201 cgcacgccat caggtcgggg atcgccttct tgtagttccc gtcgctgtga tagaggacct
     1261 tgatgccggc ctcgtgccag gcatcgacca gccgacggac gtaggggtag tggaaccgat
     1321 gaaggaactc cgggctgaag atgggcccct gcttggtgga gaaatcctcc ggcaacagga
     1381 tcagcggaga cagggcgggg tccgccaccg cccgcacccg gcggacctcg ttgtccgtgg
     1441 cgatctccat gagaagcgaa agcaccgtcg gatgctcaag cgtgaagaag ctgtaaagct
     1501 ccaggcccat tcgatcgaag acaccgcaga atccggtcat gctgaacgcg cagagcaccg
     1561 tttcaccgat cagcttctgg gtctcccgat agagcgcccg gtgggcgtcg cgttcccggt
     1621 cggcgtcgaa gagtcgagag cgctcccgct cacgttcgat gagcctgcgg acccatgcga
     1681 gcgcgccgtc cacggtgtcg aacggccggc tcacgtgcca gctcgtccag ttgtcgcgct
     1741 gactcacaaa gccgtccgga tccgtgaccc ggtcggtccc gatgggcgga acgaggggga
     1801 agcacatatc cagggtcttg cggatggcct gtccgatctc ctccgggcca taggagaatc
     1861 cctggatgcg ccggcccgtg tacatctcga tcaccgatgg gctgtagctg agctggtcgt
     1921 gaagcgcagc ccgatccacc ggttggtggc gcagcgtcag ccccacacgt tcccgcttcg
     1981 agatgcggtc tccttcgaag aggcgctcga acgttgcggc catgctcacg ccgtcgccag
     2041 gatcagcact cggtagtcca gcgccgggac cgccaccgtg agccggtccc cgtccttgcg
     2101 cagctctcct ccaaagaggt ccgcgacgga gcggaagctc gtcgcgtcca ggtcagcaca
     2161 agactcttcc ctgcccgggt tgaacaggag ccacgcttcc tggccctcgc gcacgcgctt
     2221 gcggatgagg acgttcccgg cgcgctgcgg ctgaatgccg caccgcgaca ggaggcgatc
     2281 cacgaacgcg ccggcggctg aggagcggta cgcggtaccc cgatgtccta ccacggtacc
     2341 cagcaggatg gccctgcccc tgccggcgga gcgctctacc gccgcgggca gatcaccgga
     2401 gaggaagatg gcctggctgc catcgcactc gaaggtctcc tggtacagcg acggctcgat
     2461 ggcgcatccc tggaaggctc cagcgccgac cagcgctcgg tgctccgcgt actcgcccca
     2521 cgtgcgctcg cgcggaagcc agcgctcgcc ggcgcccggc tcccgcacca ccgccaggca
     2581 gcgctgccgc accccgaaga gctttcgggc ggtcggagaa agctcgcccc gggtggcgaa
     2641 ggcatgctcg tccaatcgcc cgggacaggc ttcactcagg agcgaccctc cggcttccac
     2701 gtatcgagcc agcgcttcca
//
LOCUS       DASRZS010000538         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4528143, whole genome shotgun sequence.
ACCESSION   DASRZS010000538 DASRZS010000000
VERSION     DASRZS010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4528143"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VFH83_13075"
     CDS             complement(<1..809)
                     /locus_tag="VFH83_13075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6487352.1"
                     /translation="MRSAVRPRVLVVDDQQGLRQLLCKLLEREGFEALEAADGQQAIE
                     IFRADPPLVVVSDIMMPRMDGLALLNEVKRIDRNTTVLLMTGQGNEETLLKALRGGAS
                     NFFKKPFNVRDLVDEIRKVVDYRTEAALTTLFSPFLVEESKSFVIPTSTSPYYPIINQ
                     ITLQLPCLLPESEILNLRIGIEEMITNAIEHGNLEITFDEKNRAIENGSLAELVADRG
                     RRSDAAGRTVRVSSRLTSELFEVSVQDDGRGFDWRSLPSVAPENLLAFNGRG"
     gene            913..>2288
                     /gene="lepA"
                     /locus_tag="VFH83_13080"
     CDS             913..>2288
                     /gene="lepA"
                     /locus_tag="VFH83_13080"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET6487353.1"
                     /translation="METQRIRNFCIIAHIDHGKSTLADRFIQRARLVSDRDFHDQILD
                     TMDIERERGITIKSQAVAIPYKARDGQEYVLNLVDTPGHVDFSYEVSRSISACEGAVL
                     LIDASQGVEAQTLANLYMALEHNLEIIPVINKIDLPSADIEGVRRQIGHDLGLDADEA
                     LLVSGKLGTGVDELCEAIVQRVPPPVGSPTDPLKALIFDSHYDVYRGVIVHVRLYSGT
                     LQAGMTVRFWWNGSTYKVEETGIFRLALVPAPFLGPGEVGYLIAGIKTVSDTRVGDTV
                     TDDARPCSEPLAGFRDVKPVVFSSIYPVDANDYEELVQAMAKLKLNDASLIYEKDSSV
                     ALGYGFRCGFLGLLHLEIVQERLEREFQMSIVFTAPSVQYRVTLQGGKVEIVDNPANF
                     PDPSQIVAAEEPYISASMITPSQYLGNIITLCLEKRGTQKSMSYLDEKRVELVFEMPL
                     AEVVFDFY"
BASE COUNT          399 a          751 c          726 g          412 t
ORIGIN      
        1 ccccggccgt tgaacgccag caggttctcc ggggcaaccg aaggaagcga tcgccagtcg
       61 aatccccgac cgtcgtcctg cacgctgacc tcgaaaagct ccgaggtgag ccgcgagctc
      121 acccgtacgg tgcgtccggc cgcgtcgctg cgccggcccc gatcggccac cagctccgcc
      181 agggacccgt tctcgatggc ccggttcttc tcgtcgaagg tgatctccag atttccgtgc
      241 tcgatggcgt tggtgatcat ctcctcgatg ccgatgcgca ggttgaggat ctcgctttcc
      301 gggagcaggc acgggagctg aagggtgatc tggttgatga tggggtagta cggggaggtg
      361 gaggtgggga tcacgaagct cttggactcc tccaccagga atggcgagaa cagtgtggtg
      421 agagccgctt ccgttcgata gtccaccacc ttgcggatct cgtccaccag gtcgcgcacg
      481 ttgaacggct tcttgaagaa attggaggcg ccgccgcgaa gcgccttgag cagcgtctcc
      541 tcgtttccct ggccggtcat caggagcacc gtggtgttgc ggtcgatccg cttcacctcg
      601 ttcaggaggg cgagcccatc catgcggggc atcatgatgt ccgagacgac caccaggggc
      661 gggtcggccc ggaagatctc gatggcctgc tgcccgtcgg ccgcctccag agcctcgaac
      721 ccttccctct ccagcagctt gcagagcagc tggcgcaatc cctgctggtc gtcgacgacg
      781 aggacccggg ggcgcacggc gcttctcacg ggttgcagcc tacgggcggc ggcggtcggt
      841 gtcaagccga cgcgcgggcg cggacctcgc gcgagttgtc caaggccggc gcaccggcta
      901 tactcgtccc cgttggaaac ccagcgaata cgaaacttct gcatcatcgc ccacatcgat
      961 cacggcaagt ccacgctcgc ggaccgtttc atccagcgcg cgcgcctggt ctcggaccgg
     1021 gacttccacg accagatcct ggacaccatg gacatcgagc gggagcgagg catcaccatc
     1081 aagagccagg cggtcgccat cccgtacaag gcgcgggacg gccaggagta cgtcctgaac
     1141 ctggtggaca cgccgggaca cgtggatttc tcctacgagg tctcccgatc catctccgcc
     1201 tgtgagggcg ccgtgctgct catcgacgcc agccagggcg tggaggcgca gaccctggcc
     1261 aacctctaca tggccctgga gcacaacctc gagatcatcc cggtcatcaa caagatcgat
     1321 ctgcccagcg cggacatcga aggggtgagg cggcagatcg gccacgatct ggggctggat
     1381 gccgatgagg cgctgctcgt ctccggcaag ctcggcacgg gcgtcgacga gctgtgcgag
     1441 gccatcgtcc agcgggtgcc tccgccggtg ggcagcccga ccgacccgct gaaggctctg
     1501 atcttcgact cccattatga cgtctaccgc ggcgtcatcg tgcacgtgcg cctgtactcg
     1561 ggaacgctgc aggccgggat gaccgtccgg ttctggtgga acggctcgac gtacaaggtg
     1621 gaggagacgg gcatcttccg tctggctctg gtgccagccc cgttcctcgg tccgggagag
     1681 gtcggctacc tcatcgcggg catcaagacg gtatcggaca cccgcgtcgg ggacacggtc
     1741 accgacgacg cgcgaccctg cagtgagccg ctggcggggt tccgcgacgt caagcccgtg
     1801 gtcttctcgt ccatctatcc cgtcgacgcg aacgattacg aggagctggt ccaggccatg
     1861 gccaagctca agctcaacga cgcgtccctg atctacgaga aggactcctc cgttgccctg
     1921 ggctatggct tccgctgcgg ctttctggga ctcctgcatc tggagatcgt ccaggagcgc
     1981 ctggagcggg agttccagat gtccatcgtc ttcaccgcgc cttcggtcca gtaccgggtc
     2041 actctccagg gcggcaaggt cgagatcgtg gacaacccgg ccaactttcc ggatccctcc
     2101 cagatcgtgg ccgccgagga gccgtacatc tcggcttcca tgatcacgcc gtcacagtac
     2161 cttggcaaca tcatcacgct ctgtctcgag aagcgcggca cccagaagag catgagctat
     2221 ctggacgaga aacgcgtgga gctggttttc gagatgccac tcgccgaagt cgtcttcgac
     2281 ttctacga
//
LOCUS       DASRZS010000539         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_302186, whole genome shotgun sequence.
ACCESSION   DASRZS010000539 DASRZS010000000
VERSION     DASRZS010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302186"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VFH83_13085"
     CDS             complement(<1..645)
                     /locus_tag="VFH83_13085"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HET6487354.1"
                     /translation="MESAGTMGFSTLADLARIRTGVRRERLSSYDRAGGNYDWWVVKA
                     GESARIAEIPGPGCIRHLWMTLWSTEPAFLRKVVLRAWWDGEAAPSVLCPIGDFFGIG
                     HGIMKNFWSLPLQMSPQDGRSFNCWFPMPFSSAARLEVANDSASPINLYFYVDYDRLD
                     RLDAGYGRFHAQWRRENPTDGWGDDGLRFSEHKDVMERVWRTPNLDGAGNYVILE"
     gene            complement(663..>2202)
                     /locus_tag="VFH83_13090"
     CDS             complement(663..>2202)
                     /locus_tag="VFH83_13090"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HET6487355.1"
                     /translation="TWSFEEGKETLPSPVLDAQPSWVRAYWKAWELAYANFHQPAPGS
                     GFPSPFIDAAFNQNIFLWDTCFMTMFCNVAHPLVPGISSLDNFYAKQYPDGEIAREID
                     RTTGETFRAWQNTENAPLFSRWGWTVPWAIRRVKVKYVGREAPVANPRLTLDALDHPL
                     FAWSELEHYRWTGDSARLASFWDTLVRYYESLKTYLRQGNGLYITDWASMDNSPRNRH
                     LKGGGTGVDISAEMVLFGRQLAEIARILGRTQAAEPLEREAQELSDRINELMWSERHG
                     FYFDLSLAGRQIPIKTVAAFWTLLSGVANERQAKALVGHLRNPSTFGRLNPVPSCSAD
                     APGYDPTGMYWRGATWAPIITMVIRGLERYGENELAREIALKHVELVADVLEQTGTIW
                     ENYAPDAPRPGSLPMGRSVRKDFVGWSGIGPILYLLEYGIGLQADAPAGRLVWTIGSY
                     GRAGCERLRFGGRVVSLVAEPRVPSGARTLTVSSDGPFTLVAREDGRERTFAVTPGSQ
                     RLLL"
BASE COUNT          379 a          752 c          711 g          360 t
ORIGIN      
        1 ctccaggatc acgtagttgc cggcgccgtc caggttcggg gtgcgccaca cccgctccat
       61 gacgtccttg tgctccgaga agcgaagccc gtcgtctccc cagccatcgg tggggttctc
      121 tcgacgccac tgcgcgtgaa accgtccata gccggcgtcc agccggtcca gtcgatcgta
      181 gtccacgtag aagtagaggt tgatggggct ggccgagtcg ttggccacct ccagccgggc
      241 agccgaggag aacggcatcg ggaaccagca gttgaaggac cgaccgtcct gggggctcat
      301 ctggagcgga agcgaccaga agttcttcat gatgccgtgg ccgatcccga agaagtcccc
      361 gatgggacac aggacgctcg gcgccgcctc cccgtcccac cacgcccgaa ggaccacctt
      421 gcgcaggaac gccggttcgg tggaccacag ggtcatccag aggtgtcgga tgcagcccgg
      481 ccccgggatc tcggcgatcc gcgccgactc gccggccttg accacccacc agtcgtagtt
      541 gccgcccgcc cgatcgtagc tggagagcct ctcccgacga acgcccgttc gtatcctggc
      601 caggtctgcc agggtggaaa agcccatcgt tccagcggac tccatgatcc ctcctcgcgg
      661 ccttacaaga gcagcctctg ggagccgggg gtaacggcga aggtccgctc gcgcccgtcc
      721 tcccttgcca ccagggtgaa gggaccgtcg gagctcaccg tgagcgtccg agccccggac
      781 gggacgcgcg gctcggccac cagggacacc acgcggccgc cgaaccgcaa ccgctcgcac
      841 cccgcgcggc cgtaggaccc gatggtccac accaggcgcc cggcgggcgc gtccgcctgg
      901 agtccgatcc cgtactccag gagatagagg atgggaccga tcccgctcca cccgacgaaa
      961 tccttgcgta ccgaacggcc cattgggagg ctgccgggtc gtggagcatc gggtgcatag
     1021 ttctcccaga tggtgccggt ctgctcgagc acatccgcta ccagctcgac gtgcttcagc
     1081 gctatctccc gggccagctc gttctctccg tatcgctcaa gacctcggat gaccatcgtg
     1141 atgatcggcg cccaggtggc cccccgccag tacatgccgg tggggtcgta gcccggcgcg
     1201 tcggcggagc aggacggcac cgggttgagc cggccaaagg tcgatgggtt acgaaggtgg
     1261 ccgaccaggg ccttggcctg gcgctcgttc gccacccccg agagcaaggt ccaaaacgcc
     1321 gcgacggtct taatgggaat ctgcctgccg gccagcgaca ggtcgaagta gaagccgtgg
     1381 cgctcgctcc acatgagctc gttgatccga tcggagagct cctgcgcctc tctctccaga
     1441 ggctccgcgg cctgagtccg tcccaggatc cgggcgattt cggcgagctg acggccgaag
     1501 aggaccatct cggccgagat gtcgaccccc gtgccgccgc ccttgaggtg acggttcctg
     1561 ggcgaattgt ccatgctcgc ccaatccgtg atgtagagcc cgtttccctg gcggaggtac
     1621 gttttcagcg attcgtagta ccgaaccagc gtgtcccaga acgaggcgag ccgggccgaa
     1681 tccccggtcc accggtagtg ctccagctcg gaccaggcga acagcggatg gtccagggca
     1741 tccagggtga gccgcggatt cgcaacgggc gcctcccgac cgacatactt caccttcacg
     1801 cgccgaatcg cccaggggac ggtccagccc catcggctga agagcggtgc gttctcggtg
     1861 ttctgccagg cgcggaacgt ctctccggtg gtccggtcga tctctcgggc gatctcgccg
     1921 tccgggtact gcttggcgta gaagttgtcc agagaggaga tccccggcac caacgggtgc
     1981 gccacgttgc agaacatggt catgaagcag gtgtcccaga ggaagatgtt ctggttgaaa
     2041 gcggcatcga tgaacggcga cgggaatccg cttcccggcg ccggctgatg gaagtttgcg
     2101 taggcgagct cccaagcctt ccagtacgcg cggacccagg acggctgcgc gtccagcacc
     2161 ggagacggca gcgtctcctt cccctcctcg aagctccagg tg
//
LOCUS       DASRZS010000540         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_681483, whole genome shotgun sequence.
ACCESSION   DASRZS010000540 DASRZS010000000
VERSION     DASRZS010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681483"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..466
                     /locus_tag="VFH83_13095"
     CDS             122..466
                     /locus_tag="VFH83_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HET6487356.1"
                     /translation="MPTYEYACTNCGHTFEAMQRMSDKPLTRCPKCKGAIRRVIHGGM
                     GVIFKGSGFYTTDYKKSSAMTGGNGGSAKETSAKDASSKESSSSSSSEKTKESSKESP
                     KDGAKEKAKAST"
     gene            complement(459..1643)
                     /gene="thiI"
                     /locus_tag="VFH83_13100"
     CDS             complement(459..1643)
                     /gene="thiI"
                     /locus_tag="VFH83_13100"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HET6487357.1"
                     /translation="MDRLFLVKYGEIALKKRNRGAFVRSLKDSVRAKLPGVPVHVWET
                     FHRVFVRYDPESRERIIQSLGRTFGVVSFSDALRVDNEMPQIEDAARELARGFAAAGL
                     GGRFKAEVRRAEKSFPLTSYQIACRIGDILLTEIPGLTVDVHHPDWVLNVEIRERAYL
                     YGREYPGPAGLPTGSSGRGLLLLSGGIDSPVAGYMMAKRGLAIEAVYFHSPPFTSEMA
                     RRKVESLVRVLAGYVPGIVLHVVNFTPVLGRIRERARLEETTLLLRACMMEFSCLLAA
                     RRAAACLVTGESLGQVASQTVESMHFTGSLATLPVLRPLVGMNKEEIIALARSLETFE
                     ISNLPYPDCCVLFSPDHPLIHPDVARMTRAYQKLDVGELLTEALGQIDTVSPVTAGAD
                     AT"
     gene            complement(1643..>2494)
                     /locus_tag="VFH83_13105"
     CDS             complement(1643..>2494)
                     /locus_tag="VFH83_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6487358.1"
                     /translation="HGIRCAVVRPRTGGIVDPQDVADALDDTTRMVSVMLVNNETGAI
                     QPIAEIAQAISDFSARHGRRILLHTDAVQALGKIPLSMAALGVDAASFSGHKLGAPRG
                     VGALWIRDGVSPMFLTAGGEQEGGRRPGTENLAGIAAMTVALERRLSAMEQELTLARE
                     NEERLLTGLASLPGARIFPQERRPAGSFSPYIVCAGFPPVPAEVVVRVADARGFCIST
                     GAACSSKKKDRTRVTESMGLSHATALCAIRVSTGYSTSAEQIGAFLDALRHETAGLLA
                     VAGGRAT"
BASE COUNT          463 a          827 c          818 g          386 t
ORIGIN      
        1 ggccccgccg tccttgccct aacccgtcaa tcgggctata ttgtgaaaaa cggaagatcc
       61 tggaagcccg aacgggatcc aggagtgcgt tcgctctaga gaagaggcag gagtcgtttc
      121 aatgcccacg tacgagtatg cgtgcacgaa ttgcggccac accttcgagg caatgcagag
      181 gatgtctgac aagccgctca cccgctgtcc caagtgcaag ggtgccattc ggcgcgtgat
      241 tcacggcggg atgggagtga tcttcaaggg aagcggcttc tatacgacgg attacaagaa
      301 gagctctgcg atgaccggcg gaaacggagg ctccgccaag gagacctccg ccaaggatgc
      361 ctcctccaag gaatcgagca gcagctcttc ctcagagaag acgaaggaat catccaagga
      421 aagccccaag gacggcgcca aagagaaggc caaggcctct acgtagcgtc cgctccggcg
      481 gtcacagggc tcaccgtatc gatctggccc agcgcctcgg tcaacagctc ccccacgtcc
      541 agcttctggt acgcccgggt catgcgcgcc acatccgggt ggatgagtgg atggtcgggt
      601 gagaagagga cgcagcagtc cgggtaggga aggttcgaga tctcgaaggt ctcgagggac
      661 ctggcaaggg cgatgatctc ttccttgttc attccgacga gcggccgcag gacgggcagg
      721 gttgccaggg atccggtgaa atgcatgctc tccaccgtct ggctggccac ctggcccagg
      781 ctttccccgg tgaccaggca tgccgctgcc cgccgcgccg ccagcaggca ggaaaactcc
      841 atcatgcagg cccggagcag gagcgtcgtc tcctccaggc gtgcgcgctc gcgaatcctc
      901 ccgaggacgg gcgtgaagtt gaccacgtgc aggacgatgc cgggaacata gccggccagg
      961 acgcggacca ggctttccac cttcctgcgg gccatctccg aggtgaacgg cggggagtgg
     1021 aaatacaccg cctcgatggc cagccctcgc ttggccatca tgtagccggc cacgggggaa
     1081 tcgataccgc ccgaaagcag cagcagcccc cgcccggagc tgccggtggg caaccccgcg
     1141 gggcccggat actctcgacc gtagaggtag gcgcgctccc ggatctcgac gttgagcacc
     1201 caatccgggt gatgcacgtc taccgtcagc ccggggatct ccgtgagcag gatgtccccg
     1261 atccgacagg cgatctggta tgaggtgagg gggaagctct tctccgccct gcggacctcc
     1321 gccttgaacc gtccaccgag cccggccgcc gcgaatcccc gcgccagctc ccgcgcggca
     1381 tcctcgatct gaggcatctc gttgtccacc cgcagggcat cgctgaacga gaccacgcca
     1441 aaggtccggc ccagggactg gatgatccgt tcgcggctct ccggatcgta acggacgaag
     1501 acccggtgga acgtctccca gacgtgaacg ggcacgccgg gcagcttggc gcgcaccgaa
     1561 tccttgaggc ttcggacaaa cgcgccccgg ttgcgcttct tgagcgcgat ctctccgtac
     1621 tttacgagaa agagacggtc catcaggtgg cgcggccccc ggctaccgcc agaaggcccg
     1681 ccgtctcgtg gcgaagcgcg tccaggaacg cgccgatctg ctccgcggag gtcgaatagc
     1741 cggtcgagac acggatggcg caaagggcgg tggcatggga gagccccatg ctctccgtga
     1801 cgcgggtccg gtccttcttc ttgctggagc acgcggcccc tgtcgagatg cagaacccgc
     1861 gggcgtcggc gaccctcacc accacctccg ccgggaccgg cgggaagccg gcgcagacaa
     1921 tgtagggtga gaagctgccc gcgggccgcc gttcctgcgg gaagatccgc gcgccgggca
     1981 gcgaggcaag cccggtgagg aggcgctcct cgttctctcg cgccagggtc agttcctgct
     2041 ccatggctga aagacggcgt tccagggcca cggtcatggc cgcgattccg gccaggttct
     2101 ccgtgccggg tctgcggccg ccttcctgct ccccgccggc ggtcaggaac atcggtgaaa
     2161 cgccatcacg gatccagagc gcgccgacgc ctcgaggcgc acccagcttg tgcccgctga
     2221 aggacgcagc gtccaccccc agggccgcca tggagagcgg aatcttcccg agcgcctgga
     2281 ccgcgtccgt gtgaaggagg atcctccgac cgtggcgggc cgagaagtcc gaaatcgcct
     2341 gggcgatctc cgcgatcggc tggatggcgc ccgtctcgtt gttgaccagc atcacggaga
     2401 ccatccgggt ggtatcgtcc agggcgtcgg caacgtcctg gggatcgacg atccctcccg
     2461 tccgagggcg gaccaccgcg cagcggatcc catg
//
LOCUS       DASRZS010000541         5572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3368609, whole genome shotgun sequence.
ACCESSION   DASRZS010000541 DASRZS010000000
VERSION     DASRZS010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3368609"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VFH83_13110"
     CDS             complement(<1..661)
                     /locus_tag="VFH83_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6487359.1"
                     /translation="MKTYAIKGTPARASRIAYGCMGIGGDWSSAPLTDQTRKDAMASV
                     RTALDEGITFFDHADIYGRTKAEEAFSGIWKERPGLRPSIMVQTKCGIRFEDDPKGAP
                     ARYDYSKEHILRTVEGSLKRLGTDYVDFLLLHRPDALVEPEEVAAAFDHLASQGKVRF
                     FGVSNHNAWQIELLKRWVTQPLVVNQLEVNVLHNQLINAGVSVNQDAPAMPTHGEGTL
                     EY"
     gene            743..1273
                     /locus_tag="VFH83_13115"
     CDS             743..1273
                     /locus_tag="VFH83_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487360.1"
                     /translation="MKHRRWILRGLLVLTLLALGGCPFSSDHPLSDPGQAVRDDALVG
                     SWRWQDPDTRHWVTLRIYPYNERELVGVTRDEGEPQARIDAYRLFSTTVGGQQFLNAQ
                     ELTPDANHEWYLARYTAAGNQLTLAFVDDALFQSRTFDSADALRRFLEQNLANPKLYA
                     TESGEPFQSVWQRAPW"
     gene            1394..1735
                     /locus_tag="VFH83_13120"
     CDS             1394..1735
                     /locus_tag="VFH83_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487361.1"
                     /translation="MAEEARAWFDEAARQPVPVRDLVLLYYRTGSPIETTLRGEPDAA
                     LACLRGGWKHLVDLNLRVVPENDPVQNAAPLVRENEMVILVRAAATDGRTALAIVYPY
                     LTLEPIIEILD"
     gene            1739..2401
                     /locus_tag="VFH83_13125"
     CDS             1739..2401
                     /locus_tag="VFH83_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6487362.1"
                     /translation="MAPGPESLSEQLRRVRPFNRLGERDLRSALRLFEQTTVRKGKAL
                     YYQGDRSEGCWLLLDGRTRGMMYRTDESILSLGFSLPGDWLGIAEMLLSSTYLNDALT
                     EESCSLAHIARTSFDRLLAIGTLREFFLREMARRYYVLHSRLELATPMDRLVGYLLEH
                     CTGDEPLSLSVTQEELAEAIGVSRETVNHHLALLGERGAVKSGRGLVEVPDPGALRRL
                     QR"
     gene            complement(2392..4635)
                     /locus_tag="VFH83_13130"
     CDS             complement(2392..4635)
                     /locus_tag="VFH83_13130"
                     /inference="COORDINATES: protein motif:HMM:NF009881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6487363.1"
                     /translation="MRKAPIAADLFDQEPSVTGRGQPLAARMRPRTLQEFVGQEHILG
                     AGRLLRRAIQADQLSSLIFYGPPGTGKTTLARVIANTTRSHFQSLNAVLAGVKDIREA
                     TAAADERRKLYSRRTILFVDEVHRWNKAQQDALLPWVENGTIVLIGATTGNPYFEVNP
                     ALVSRSRIFQLVPLTEADLFAVARAALADPERGYGGIDVRIEEDALAHLVSISSGDAR
                     TLLNAVELAVETTPRTFPPPAGETVVITRAIAEESIQRRVVLYDKEGDYHFDTISAFI
                     KSLRGSDPDASLYWLARMVYAGEDPRFIFRRMMILASEDVGMADPQALVFVEAAAAAF
                     ERVGFPEGRYFLAHAVLRLATAPKSNTAMGFFDALDTVEKEHTDTVPVHLKDENRDSE
                     GFGHGKGYLYPHAYRDHWVAQQYLPDGLQGRLFYQPSDQGFEAGIQAQVQRRREAQLE
                     AMREAEDRPVEVLTFSKAEAGRDLWLERTSSGASERLAKLRDRLLDSLALARHHLVLD
                     LRAGEGLLTWEALRRVPEGGVWSLARSAAEAAGIEGRAAELPELERPRLLIGALGELP
                     QLLAALGPDAPAFDAAVGRDCLLREPDKAACLTAVARCIRPGGRLSVAEIMPGKGQRL
                     SDLLDLSDLEDRARQRILDAEARIYGSGDNPLVSWNEQTLSAAATQAGLTVRACRVEL
                     AESNRRVRPQDVDRWFSGEPGSYGALASVVLSEPELSDLARRCRERLSGRDVPWRSAV
                     VYLEAQR"
     gene            complement(4658..5185)
                     /locus_tag="VFH83_13135"
     CDS             complement(4658..5185)
                     /locus_tag="VFH83_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487364.1"
                     /translation="MENRQIGSMVGGGLVILLGILLLLDRVFPDTLHLFSWPLIILAV
                     GAIFFASGLASRAAGLVIPGTIIAGIGGILFWQNATGRWFSWAYVWTLIPGFVGLGLL
                     VFGWQYYAGQTQTGRSVRLAARWLIGISIGLFLIFTTFLGPFPWFWAILGAGLVVLGV
                     ILVARSMRGRPARTE"
     gene            complement(5468..>5572)
                     /locus_tag="VFH83_13140"
     CDS             complement(5468..>5572)
                     /locus_tag="VFH83_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6487365.1"
                     /translation="SVEDVAAAFVYLASDEARFVNGVSFPVDGGLTAS"
BASE COUNT          890 a         1910 c         1866 g          906 t
ORIGIN      
        1 aatactccag ggtcccctcg ccgtgggtgg gcatggcggg ggcgtcctgg tttacggaca
       61 ccccggcgtt gatgagctgg ttgtgcagga cgttgacctc gagctggttc accacgagcg
      121 gctgggtgac ccagcgtttc aggagctcga tctgccacgc attgtggttg ctcacgccga
      181 agaaccggac ctttccctgc gacgccaggt ggtcgaacgc ggctgccacc tcttcgggtt
      241 ccaccagggc atccggccgg tgcaggagca ggaagtcgac gtagtcggtg ccaagccgct
      301 tcaggctgcc ctccacggtc cgcaggatgt gctccttgct gtagtcgtag cgcgcgggcg
      361 cgcccttggg gtcatcctcg aaccggatgc cgcacttggt ctggaccatg atggaaggcc
      421 gaagcccggg gcgctccttc cagatgccgg agaaggcctc ttccgccttg gtcctcccgt
      481 agatgtccgc gtggtcgaag aaggtgatcc cctcgtccag agccgtgcgg acggacgcca
      541 tggcatcctt gcgggtctgg tcggtcaacg gagcgctgct ccagtccccg ccgatcccca
      601 tgcagccgta ggcgatccgc gaggcgcgcg ccggcgttcc cttgatcgcg taggtcttca
      661 tcggcatacc tccaggtggt tggtcttccc tgaatgtacg gtccctgcgg ggaaggagca
      721 acccttgtgg tacaatcgcc tcatgaaaca ccggcgatgg atccttcggg ggctcctggt
      781 cctcacgctc ctcgccctgg ggggctgccc gttcagctcg gaccatccgc tctccgaccc
      841 ggggcaggcg gttcgggacg acgccctggt gggctcctgg cgatggcagg acccggacac
      901 ccggcactgg gtcacgcttc gcatctaccc gtacaacgag cgcgagctcg tgggggtgac
      961 ccgtgacgaa ggggagccgc aggccaggat cgatgcctat cggctcttca gcacgacggt
     1021 gggcggtcag cagttcctga atgcccagga gctgaccccg gatgccaacc acgaatggta
     1081 cctggcgcgc tacacggcgg ccggcaacca gctcacgctc gccttcgtcg acgacgcgct
     1141 cttccagtcc cggaccttcg actcagccga cgcgctgcgc cggttcctgg agcagaacct
     1201 tgccaacccg aagctctatg ccaccgaatc cggggagccc ttccagtccg tctggcagcg
     1261 cgcgccctgg tgaggtccga acgccgcgct gatagccgag aacctccctc gacacgcgcg
     1321 gtcggctacg gaggcgggcc ggtgggcttc accgggcaag ctcttcctgg agcctgacca
     1381 ccccgccaac ccgatggcgg aggaggcgcg cgcctggttc gacgaggcgg cccgccagcc
     1441 ggtgccggtg cgcgacctgg tgcttctcta ctatcgcacc ggaagcccga tcgagacgac
     1501 gctcagaggc gaacccgacg ccgcgctggc ttgtctccga ggcggctgga aacacctggt
     1561 ggacctcaac ctgcgcgtag tgccggagaa cgaccccgtc cagaatgcgg cccctctggt
     1621 gcgggagaac gagatggtga tcctggtccg agccgcggcc acggatggtc gaacggcgct
     1681 tgccatcgtc tatccgtacc tcactctcga gccgatcatc gagatcctgg attgagccat
     1741 ggcgccgggg ccggagagtc tctcggagca gcttcgccgg gtgcggccgt tcaatcgcct
     1801 gggagagcgg gacctgcgct ccgcgctgcg cctcttcgag caaacgaccg tccgaaaggg
     1861 caaagctctc tactaccagg gagatcggtc ggaaggctgc tggctcctcc tggacggccg
     1921 gacgcgcggg atgatgtatc ggaccgacga gtccatcctc tcgctggggt tttcgcttcc
     1981 gggagactgg ttggggattg ccgagatgct gctctccagc acctacctca acgacgcgtt
     2041 gacggaggag tcctgcagcc ttgcccatat cgcgcgaacc agctttgatc gtctcctggc
     2101 gatcggcacg ctgcgcgagt tcttcctccg cgaaatggcc cggcgatact acgtccttca
     2161 ctcgcgtctg gagctggcca cccccatgga ccgcctggtc ggctacctgc tggaacactg
     2221 cacgggagac gaacctctgt cgttgagcgt gacccaggaa gagctggccg aagccattgg
     2281 ggttagccgt gagaccgtga accatcacct ggccttgctc ggtgagcggg gtgccgtgaa
     2341 atccggcagg ggcctcgtcg aggtgccgga ccccggcgct ctccggcggc ttcagcgctg
     2401 agcttccagg tacaccacgg cgctgcgcca cggaacgtcg cggccgctca ggcgctcccg
     2461 gcaccgtcgt gccaggtcgg acagctccgg ctcggagagg actacggacg ccaaagcgcc
     2521 gtagcttccg ggctcgccgg agaaccagcg gtccacgtcc tgcggccgga cccgccggtt
     2581 ggattccgcc agctcaacgc ggcaggcgcg gacggtgagt ccggcctgag tggcagcggc
     2641 ggacagcgtc tgctcattcc aactgaccag ggggttgtcc ccggatccgt agatccgcgc
     2701 ctcagcgtcc aggatacgtt ggcgtgcccg gtcttccagg tccgagagat cgagaaggtc
     2761 ggagagccgc tgccccttgc cgggcatgat ctccgccacg gaaaggcgcc cgccggggcg
     2821 gatacagcgt gccacggcgg tcaggcaggc agccttgtcc ggctcccgaa gcaggcagtc
     2881 tcggcccacg gcggcatcga acgccggtgc gtcgggtccg agggcggcga gaagctgggg
     2941 cagctccccc agcgctccga tgagcaggcg tggccgctcg agctctggca gctccgccgc
     3001 gcgcccctcg atgccggccg cctcggcggc ggatcgggca agcgaccaca cgccgccctc
     3061 gggcacccga cgcagggcct cccaggtcag gagcccttcc cccgcccgaa ggtccagcac
     3121 caggtggtgg cgcgcaagag caagggaatc caggagccga tcccgaagtt tcgccagccg
     3181 ctcgctggct ccgctcgagg tccgctccag ccagaggtcg cgtccggcct ccgccttgct
     3241 gaaggtgagc acctccacgg gccgatcctc cgcctcgcgc atggcctcca gctgggcttc
     3301 cctccgccgc tggacctggg cctggatgcc ggcctcgaac ccctggtccg aaggctggta
     3361 gaagagccga ccctgaaggc cgtccggcag gtattgctgg gccacccagt gatcccgata
     3421 ggcatgcgga tagaggtacc ctttcccgtg cccgaaaccc tcgctgtccc ggttctcgtc
     3481 cttgaggtgc accggaacgg tgtcggtgtg ttccttctct acggtgtcca gcgcgtcgaa
     3541 gaatcccatg gcggtgttgg acttgggcgc ggtggccagc cgcagcacgg cgtgcgccag
     3601 gaagtaccgg ccctcgggga atcccacccg ctcgaacgcc gcggctgccg cttcgacgaa
     3661 gaccagcgcc tggggatccg ccatgccgac gtcttcggag gccaggatca tcatccgccg
     3721 gaaaatgaac cgcgggtctt cccccgcgta gaccatccgt gccagccagt acagggacgc
     3781 atccgggtcc gatccccgca ggctcttgat gaaggcgctg atggtgtcga agtggtagtc
     3841 accctccttg tcgtagagca ccacgcgccg ctggatgctc tcttccgcga tggctcgggt
     3901 gatgacgacg gtctcaccgg ccggaggcgg gaaggtcctc ggcgtggtct ccaccgccag
     3961 ctccacggca ttcaggagcg tgcgggcgtc tccgctggag atgctcacca ggtgggccag
     4021 agcgtcttcc tcgatgcgga cgtcgatgcc accgtacccg cgctcgggat cggcgagcgc
     4081 cgcccgggca accgcaaaga gatcggcctc ggtcagcggg acgagctgga agatccggga
     4141 ccggcttacc agcgccgggt tcacctcgaa ataagggttg ccggtggtgg cgccgatgag
     4201 gacgatggtc ccgttctcca cccacggcaa gagcgcgtcc tgctgggcct tgttccagcg
     4261 gtgcacctcg tcgacgaaca ggatggtcct tcggctgtag agcttgcgtc gctcgtccgc
     4321 cgcggcggtg gcctcccgga tgtccttcac gccggccagc accgcgttga ggctttgaaa
     4381 gtggctcctc gtggtattgg cgatgaccct ggccagggtg gtctttcccg tcccgggagg
     4441 cccgtagaag atcagggagg agagctggtc cgcctggatg gcccggcgca ggagacggcc
     4501 agcacccagg atgtgctcct ggcccacgaa ctcctgcagg gtgcgcggcc gcatgcgcgc
     4561 cgccagcggc tgcccccgcc cggtgaccga tggctcctgg tcgaagagat ccgcggcgat
     4621 gggggctttc ctcatgcggc cagcgcgggc gccggcgcta ttccgtccgg gccgggcggc
     4681 ctctcatgct gcgcgccacc aggatcaccc ccagcaccac cagccccgcg cccaggatgg
     4741 cccagaacca ggggaacggt ccgaggaacg tcgtgaagat caggaagagc ccgatgctga
     4801 tcccgatcag ccaacgggcg gccagccgca cgctacgccc cgtctgggtc tggccggcgt
     4861 agtattgcca gccgaacacg agcagcccca gtcccacgaa cccggggatc agcgtccaca
     4921 cgtaggccca gctgaaccag cgacccgtgg cgttctgcca gaagaggatt cccccgatcc
     4981 ccgcgatgat ggtcccgggg atgaccagcc cggcggcccg gctcgccaag ccgctggcga
     5041 agaagatcgc gcccacggcc aggatgatta gcggccagga gaacaggtgg agggtgtccg
     5101 ggaagacgcg atccaggagc aggaggatcc ccaggaggat gaccaacccg cccccaacca
     5161 tcgaaccgat ctgtcggttt tccatgggcc gatcatcgac cgggcgccag ccggtgtcaa
     5221 ctcggcggac gggtgtagtc caaggagcgg gctttttcga tgggtctctg gcaaccccgg
     5281 cgccggctcg acctgcccgg cgactcgggt ctcgcggacg tggcctgtgg caccgttccc
     5341 gcggaggcgc tgaggcgcgg acgtgcccaa cggtaccttt gcgcatgttg gcgaacgtct
     5401 cctcgcgggc aaaaccggac gacactgtgg tcggttttcc ttcgcgcagt gaaacccgcg
     5461 ctggcgctca actggccgtg agaccgccgt ccaccgggaa ggagacgccg ttcacgaacc
     5521 gcgcctcgtc ggaggccagg tacacgaagg ccgcagcaac gtcctccacg ga
//
LOCUS       DASRZS010000542         5424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5685372, whole genome shotgun sequence.
ACCESSION   DASRZS010000542 DASRZS010000000
VERSION     DASRZS010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5685372"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1252
                     /locus_tag="VFH83_13145"
     CDS             188..1252
                     /locus_tag="VFH83_13145"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6487366.1"
                     /translation="MSDVSEIVGRSELFMGLGKASIERVAEQLQRVSFAKGSVICREG
                     EPGTSMFVIVEGRVSVSKETGWGRRELQQMGPREAFGEMALITQEARSASVAALEPTE
                     CLKLQQEGFSVLLDQDPLFAQRVAKVLTRRIAALDRKTSEELLGAYRALMFGFADLTD
                     SRDPETGAHLERTRAYCVRMTEMMSVHPRYRERMSPDFIDEMYNLSPLHDIGKVAVPD
                     AILLKPARLTAEEFEIMKTHTTAGAAAFQKVLEQCKAEVFVMAQRICLHHHEKWDGSG
                     YPSHLAGDAIPLEARIMAMADVYDALLSKRVYKPAWTYEAAREEIRKSAGTFFDPVMT
                     QIMLEDIHRFEEIHRAHQDP"
     gene            complement(1286..1606)
                     /locus_tag="VFH83_13150"
     CDS             complement(1286..1606)
                     /locus_tag="VFH83_13150"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET6487367.1"
                     /translation="MPEQEMQGSPFLSTIARLDPAFMEQVRRTEALVYSDGALPRKFK
                     LLVAMAFDATHGAVSGVKSLAAAAMKAGATKEEVAEVLRVAAYLGGAGSLYSASQGLK
                     ELFP"
     gene            1724..2515
                     /locus_tag="VFH83_13155"
     CDS             1724..2515
                     /locus_tag="VFH83_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6487368.1"
                     /translation="MGLLDGKVALITGASTGMGRAIAERLAAEGAALGLVARGREKLE
                     EAAARARDLGGPAFSFPGDVSERAFAKRVVDQMVERLGRIDLLVNNAGTNTFHRNLAD
                     ITEADWRRVMDTNITGVFLFTRYVLRPMRRAGRGQIISISSTAGIAPNAPSGIAYSAS
                     KSALHSFTTSVNLEERPHGIRACVIAPGETDTPNLDLRPLPPSAEALAKMLRAEDIAN
                     AVLYVASQPEHVSVELLVLTPTVRRNYLADYDRYVAEGHTDVEPD"
     gene            2534..4948
                     /locus_tag="VFH83_13160"
     CDS             2534..4948
                     /locus_tag="VFH83_13160"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET6487369.1"
                     /translation="MPSDLSGSIRLTGESSIPNVIAQLTLLEKASLCGGATAFRTHGV
                     ERLGIPPLVMADGHNGINIFHLLHNYVAEAAARLGVEPRTIDPVAARLRAGGREALGA
                     LLSQPQADPSGRALAERLRKELPAAGLPSCFPTGIVMAATWNPDLVFECGRAVAREAL
                     AFGMDVVLGPNVNIHRDPLGGRVFESYSEDPFVAARIGVAYIRGMQSEGVAAVVKHFA
                     ANNQESERLGVDELISQRALQEIYFPAFKAAVQEAGVWMVMSAYNKVNGVACALNRGL
                     LTDLLRSQWGFAGFVVSDWGAAYDRVAALRAGNDLEMPGPRDPQQIVDAVNRGELSES
                     VLDQRVAAILRVMVKLPAFQFPGRPRPALDRAASASVARRVAAEGMVLLKNDRATLPL
                     PAGRIAVFGENARDPFPTGTGSAGVVAPYCVSFLEGLRTRYGAANVSFDELPQNADLV
                     VLCVGEGSGEGTDRASMRMAAGTTQRVLEVARRSRQAGKRLAVILNVCAPVEIAEWVD
                     RVDAVLLAWMAGMEMGNAAADIVSGDVSPSGKLPLTWPRRHEDTPTFTSFPGEFGESV
                     YGEGIFVGYRYYDSAGVSPQFPFGHGLSYSTFQLSGLTLSSSTVSLEGGTITVSVDIE
                     NTGDRRGAEVVQLYIHDARSSVRKPEQELKAFAKAELDPHQKRTVSFSLTADALAHYD
                     TRKARWCVEPGSFEVRVGTSSRNTPLRAEFRAVGPNPYGYGLSTAIGTLMADQRSRAV
                     LERNLPPGTIQDQAVLDFVKYAPFVPLGQALSIRLADRLRELSAEERERMRDRIVDEL
                     SKIEVE"
     gene            complement(4949..>5424)
                     /locus_tag="VFH83_13165"
     CDS             complement(4949..>5424)
                     /locus_tag="VFH83_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487370.1"
                     /translation="DLRVSSVTSRVPLRKLTEGELDQARRQAAGEKVEVDPRWQRFGP
                     DDVYARSLLELDQRYRRSPFQELEIQAMRIGEVALTAVPAELFTWNGMRIKTGSPVKP
                     AYVVTCANGMIGYVGTPDAYGRGGYEVTPCIWSKVDPAAPDVLIRDALKALSGVA"
BASE COUNT          897 a         1757 c         1865 g          905 t
ORIGIN      
        1 cacgctgcga ctgtcgtgac cgcgcgcgtc ggaggccggg ccgatggtcg cgatgggatg
       61 cgtaatcgtg ctgcagcctc tcaccgttga cctgggagga gagccgcgcg cgtgctatga
      121 tccgaagcag gttgtcttcc cggcggctcc cgcagtcttc ccgtcagcgc gaggttggag
      181 gtgatggatg tcggacgtct ctgagatcgt cggccgcagc gagctcttca tggggctggg
      241 gaaggcttcc atcgaacggg tggcggagca gcttcagcgg gtctccttcg ccaaaggcag
      301 cgtcatctgc cgggaagggg agccgggcac cagcatgttc gtgatcgtgg agggccgggt
      361 ctccgtatcg aaggagacgg gatggggccg acgggagctg cagcagatgg gaccccggga
      421 ggcattcggg gagatggcgc tcatcaccca ggaggcgcgc tcggcgagcg tggcggccct
      481 tgagcccacc gagtgtctga agctccagca ggaaggcttc tccgtccttc tggaccagga
      541 cccgctcttc gcccagcggg tagccaaggt cctgacccgg cgcatcgcgg cgctggatcg
      601 caagacctcc gaggagcttc tgggcgcgta tcgcgccctc atgttcgggt tcgcggacct
      661 gaccgactcc cgcgatcccg agaccggggc tcacctggag cggacccgcg cctactgcgt
      721 gcgcatgacg gagatgatgt cggtccaccc gaggtatcgg gagcggatgt ccccggactt
      781 catcgacgag atgtacaacc tgtcgcccct ccacgacatc gggaaggtgg ccgtgcccga
      841 cgccatcctc ctgaagccgg cccgactcac ggcggaagag ttcgagatca tgaagaccca
      901 caccacggcc ggcgccgccg cctttcagaa ggtcctggag cagtgcaagg ccgaggtctt
      961 cgtcatggct cagcggatct gtctccacca ccacgagaag tgggacggaa gcggttaccc
     1021 ctcccacctc gccggggacg ccatcccttt ggaggcgcga atcatggcga tggcggacgt
     1081 ctacgacgcc ctcttgtcca agcgcgtgta caagcccgcg tggacctacg aggcagcgcg
     1141 ggaggagatc cgcaaatccg cggggacgtt tttcgatccg gtgatgaccc agatcatgct
     1201 ggaagacatc caccgcttcg aggagatcca ccgcgcgcac caggacccgt gaggctgtcc
     1261 cgagcccgtc cgtcgacggg ccagctcagg ggaacagctc cttcagcccc tgggaggcgg
     1321 agtaaagcga gccggcgcca cccaggtaag ccgccacgcg cagcacctcg gccacttctt
     1381 cctttgtggc gcctgccttc atggcggccg cggccagcga cttcaccccg cttacggcgc
     1441 cgtgcgtggc atcgaaggcc atggcaacga gaagcttgaa cttccgaggc agcgcgccgt
     1501 cggagtagac cagcgcctcg gtcctccgga cctgctccat gaaggcgggg tccagcctgg
     1561 cgatggtgct cagaaacggc gagccctgca tctcctgttc cggcatgtca tcctccttgt
     1621 cggggtttgc gatcccatcc taagccgatc cggggcgtgg atcaacaaac ggccgctgga
     1681 acctgcgtag aaaaagctgt tatggtacga atggaggtgc ggcatgggac tcctggacgg
     1741 caaggtggcc ctcatcacgg gagcgagcac ggggatgggc cgggcaatcg ccgagcggct
     1801 ggcagcggag ggcgccgccc ttggcctggt ggcccggggc cgcgaaaagc tggaggaagc
     1861 cgcggcgcgg gcgcgggacc tcggcggacc ggcattctcg ttcccggggg acgtgtccga
     1921 acgcgcgttc gccaagcgcg tggtggacca gatggtggag cggctcggca ggatcgacct
     1981 gctcgtcaac aacgccggca ccaacacctt ccatcgcaac ctggcggaca ttaccgaggc
     2041 cgactggcgc cgggtgatgg atacgaacat caccggcgtg ttcctcttca cgcggtacgt
     2101 tctgcgcccc atgcggaggg cgggacgggg ccagatcatc tcgatctcct ccacggcggg
     2161 cattgcaccc aacgccccgt ccggaatcgc ctactccgcc tccaagagcg ccctccattc
     2221 cttcacgacc tccgtcaacc tggaggaacg ccctcacggg atccgcgctt gcgtcatcgc
     2281 gccgggggag acggacaccc ccaacctgga tctgcggccg ctgccgccgt cagcggaggc
     2341 actggcaaag atgctgaggg ctgaggacat cgccaacgcg gtgctgtacg tagcgtcgca
     2401 gcccgagcac gtgtcggtgg agctcctggt cctcaccccg acggtgcgtc gcaactacct
     2461 ggcggactac gatcggtacg tggcggaagg gcacacggac gtggagccgg attgaccggg
     2521 gcgggagggc cgcatgccga gtgatctttc ggggagcatt cgactgaccg gggagagctc
     2581 gatccccaac gtcatcgcgc agctcacgct gctggagaag gccagcctgt gcggtggcgc
     2641 cacggcgttt cggacccacg gggtggagcg cctcgggatt ccccctcttg taatggcaga
     2701 cgggcacaac gggatcaaca tcttccacct cctgcacaac tacgtggcgg aagccgccgc
     2761 gcgtctgggt gtggagccgc gaaccataga ccctgttgcc gcccggcttc gcgccggtgg
     2821 gagggaggcg ctgggcgccc ttctgtcgca gccgcaggcg gacccctcgg ggcgtgccct
     2881 cgccgagcgg ctgcggaagg agctccccgc ggccggtctt ccatcgtgtt tccctacggg
     2941 catcgtaatg gcggcgacgt ggaacccgga cctggtcttt gaatgcggcc gtgccgtcgc
     3001 ccgggaggcc ctcgccttcg gcatggacgt cgtactcggg cccaacgtga acatccaccg
     3061 ggaccctctg ggcggacggg tcttcgagag ctactccgag gaccccttcg tggccgcccg
     3121 gatcggcgtg gcctacatcc gggggatgca aagcgaaggg gttgccgcgg tcgtgaaaca
     3181 cttcgcggcc aataaccagg agagcgaacg gttgggggtc gatgagctca tctcccagag
     3241 ggctctgcaa gagatttact ttcccgcgtt caaagcggcc gtccaggagg ccggcgtctg
     3301 gatggtcatg agcgcctaca acaaggtgaa cggcgtggct tgcgctctca atcggggttt
     3361 gctgacggat ctcctgcggt ctcagtgggg gtttgcggga ttcgtggtct ccgactgggg
     3421 cgccgcgtac gaccgggtgg cggcccttcg cgccggcaac gacctggaga tgcccggtcc
     3481 ccgggatccc cagcagatcg tggatgccgt gaatcgcggc gagctgtcag agtccgtgct
     3541 ggaccagagg gttgccgcca tcctgcgcgt catggtcaag ctgccggcgt ttcagttccc
     3601 aggcaggccg cgcccggccc tggaccgggc cgcctccgct tccgtggcgc gccgcgtggc
     3661 cgcggagggg atggttctgc tcaagaacga ccgggccacc ttgcccctcc cggccggccg
     3721 cattgcggtg tttggtgaaa acgcccggga tccattccct acgggcacgg gcagcgcggg
     3781 ggttgtagcc ccctactgcg tctcgttcct ggaaggattg cggactcggt acggggcagc
     3841 caacgtgagc ttcgacgagt taccccagaa cgcggacctc gttgtcctct gcgtgggaga
     3901 gggcagcgga gagggaacgg accgggcgag catgcggatg gcggcgggga cgacccagcg
     3961 cgtcctggag gtggcccgaa ggagccggca ggcgggcaag aggctcgcgg tcattctgaa
     4021 cgtgtgcgcc ccggtggaga ttgccgagtg ggtggaccgt gtcgacgcgg tcctgctcgc
     4081 gtggatggcc ggcatggaga tggggaacgc tgccgcggat atcgtctcgg gagacgtgtc
     4141 gccatccggc aagctccccc tcacgtggcc gcggcggcac gaggacacgc cgacgttcac
     4201 cagcttcccc ggagagttcg gagagtccgt ctacggggag ggcatctttg ttggctaccg
     4261 gtactatgac tccgccggcg tttcgcccca gtttcccttt gggcacgggc tgtcctacag
     4321 cacgttccag ctttcggggc tgaccctgag ctcgagcacg gtgagcctag agggcgggac
     4381 catcacagtc tcggtggaca tcgaaaacac gggggatcgg cgcggagcgg aagtggtcca
     4441 gctttacatc catgacgcgc gaagctcggt ccgtaaaccc gagcaagagc tgaaggcgtt
     4501 cgccaaagcc gagctggatc ctcaccagaa gcggacggtt tccttctctc tcacggcaga
     4561 cgccctcgcg cactatgaca cgcggaaggc acgctggtgc gtggagccgg gcagcttcga
     4621 ggtccgagtg ggtacgtcgt cccgaaacac tcccctgcgc gcggagtttc gggcagtcgg
     4681 gcccaaccct tacgggtacg gcctctctac ggcgatcggc acgctcatgg cagaccagcg
     4741 ttcccgagcc gtcctggagc gcaacctccc gcccgggacg attcaagatc aggcggtgct
     4801 ggacttcgtg aagtatgccc cgttcgttcc gctggggcag gcgctctcga tccgtctggc
     4861 ggatcggctg agggagctgt cagcggagga gagggagcgt atgcgggatc ggatcgtcga
     4921 cgagctgtcg aaaatcgagg tagagtgact acgcgacgcc ggacagggcc ttgagcgcat
     4981 cccggatcag gacatccggg gccgcggggt ccaccttgct ccagatgcac ggcgtcacct
     5041 cgtagccgcc ccggccgtag gcgtccggtg tgccgacata gccgatcatc ccgttggcgc
     5101 aggtgaccac atacgcgggc ttgaccgggc tccccgtctt gatcctcatg ccgttccagg
     5161 tgaagagctc cgccggcacg gctgtcaggg caacttcccc gatccgcatg gcctggatct
     5221 cgagctcctg gaatggcgat cttcggtacc gctgatccag ctccagcagg gacctcgcgt
     5281 agacatcgtc cgggccgaac ctctgccagc ggggatccac ctccaccttc tctcccgcag
     5341 cctggcgccg cgcctggtcc agctcccctt cagtgagctt gcgcagcggc actcgggagg
     5401 tgacggagga aaccctcagg tcgg
//
LOCUS       DASRZS010000543        10047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5309611, whole genome shotgun sequence.
ACCESSION   DASRZS010000543 DASRZS010000000
VERSION     DASRZS010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5309611"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFH83_13170"
     CDS             complement(<1..678)
                     /locus_tag="VFH83_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006510959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET6487371.1"
                     /translation="MGKTLLSKVWDLHKVGTLPSGQDQLFIGLHLVHEVTSPQAFQAL
                     RDRGLKVLFPNRTFATVDHIVPTDGQKRPFADELAEAMMKAVEDNTRANGIAFFGLGS
                     AEQGIVHIIGPELGLTQPGMTIACGDSHTSTHGALGSLAFGVGTSQIRDILATQTLAL
                     SRPKVRRIDVSGLFASGVGAKDLVIRIIADLGVNGGIGFAYEYSGPAIEKLPMEARLT
                     LCNMSIEA"
     gene            complement(682..1194)
                     /gene="ilvN"
                     /locus_tag="VFH83_13175"
     CDS             complement(682..1194)
                     /gene="ilvN"
                     /locus_tag="VFH83_13175"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET6487372.1"
                     /translation="MSPATSRAVRRHLVSLYVANQAGVVNRVAQVFARRGFNIDSIVA
                     SPAHDPAFSHMNIVASGDEKTLEQILKQLNKLVDVVHGRDITAEDVIQRELGLFKVRC
                     EATRRTEVLDVAHAFKCQVVDLAETAITLQVTGRTEKIDALHRMLNPYGVIEMVRTGK
                     VLMARGEEVT"
     gene            complement(1191..2894)
                     /gene="ilvB"
                     /locus_tag="VFH83_13180"
     CDS             complement(1191..2894)
                     /gene="ilvB"
                     /locus_tag="VFH83_13180"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HET6487373.1"
                     /translation="MKRSDVRCTGAQAVVHILEKQGVKYVFGLPGGAAIPLFDALVDS
                     DIQLVLVRHEQGATHMADGFARASGEPGVVLVTSGPGATNTITGLLTAHMDSVPMVVI
                     TGQQSVANLGLDTFQEADVSGIALPVVKHSYLVKDATKLPRMLREAFYISKTGRPGPV
                     LVDIPKDCSNTRIDVDDQEEFELPGYRVPKEGDPVEIRRAAELLTKAKRPVLLVGHGA
                     LISGAGDELRAFAETMRIPVTSTLLGKGAFPETHLLSLGMLGMHGTAYANKAVAGCDL
                     ILSVGSRWDDRITGDLDQFCTDAVKIHVDIDPAEINKVVQVDCAIVGDARTVLAELNQ
                     HLVPGDTVQWLEQIDRWKTVHPLAYNPRGGFKAQHVIDEIYKLTGGKAIVTTDVGQHQ
                     MWAAQFYRTDERFKWISSGGAGTMGFGFPAAIGAQLARPGELVVAVVGDGGFQMTMAE
                     LSTAVNLKLPIKILIINNRYLGMVRQWQNLFYENRLSGVDLAGNPDFVKLAESYGCKA
                     VRIRRASEVTRRLQEALDYNDGPVVIDAEVVKEDNVFPMIPAGAALKDMILEPPRKVR
                     S"
     gene            3002..4585
                     /gene="lysS"
                     /locus_tag="VFH83_13185"
     CDS             3002..4585
                     /gene="lysS"
                     /locus_tag="VFH83_13185"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF014031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET6487374.1"
                     /translation="MPYKEWPFIEAQRIADRARSMGKSSVTAETGYGPSGLPHIGTFG
                     EVARTSFVLQALRTVAPEIQSRLLAFSDDMDGLRDVPLNLPNAAMLREHLGKPLTAIP
                     DPFGKEKSFAHYMNGKLREFLDSFGFEYQFASSTEQYASGVFDDGLRRVMANYEAIAE
                     LFRATIAEEKRAAWSPFFPICESCGRVYSTRVTSYDAQALTVSYVCDAAVQGKYGSCG
                     HNGTTSILGGRCKVGWKVDWALRWYSLGIDYEMHGEDLLESSRLSSRIVTILGGRPPE
                     LFKYELFLDEKGKKISKRIGNGISIEQWLRFAPVDCLLYLMFTKPQQAKRMGLPILPE
                     IVDAYLELVASDEGSEDSPIPFITRLSKGGHAGKLGDHKVITYSLILELILALNQDDP
                     VIARDYLLKFQPDIAGNIHYYEQLIGNALAYFREVIVPGRTAEKADHASDTAIAALRD
                     ELVRLRDSGAAATDWDALQTLVFQVAKDRDIKTKDWFRTLYRVFLGQSSGPRIGSFIA
                     LLGYGKSIERLEAHLATKG"
     gene            4589..5875
                     /locus_tag="VFH83_13190"
     CDS             4589..5875
                     /locus_tag="VFH83_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET6487375.1"
                     /translation="MAGKSGDLKTIQQAESVLGGEKKTGWMGRVLPFLGPAFIACVAY
                     IDPGNFATNVQGGSEFGLMLLWVILAANLMAMLLQSLSAKLGIATGKNLAEQCRERLP
                     GWLVVVMWIVAEIAAMATDLAEFLGATLGFALLFGIPLWLGVILTAIATFVILSLERF
                     GFRPLEAVITALVGVIAVCYVVEMGLVRPPLGQILLHSVVPQLSGTESVLLAVGIMGA
                     TVMPHVIYLHSSLTQSRIVVRDPKRLRRLFRFEITDVVIAMGLAGIINMAMLIMASQT
                     FHVHGMTSIATIPDAHRTLVPLLGPAASWVFAISLLASGLSSSSVGTMAGQVIMQGFI
                     RRRIPAWIRRLVTMVPSVLVILLGIDPTRALVLSQVVLSFALPAPVIPLVFFTRRKDI
                     MGVLVNKPLTTIAAGAAGIVILALNVYLLVQTFTGG"
     gene            5877..6803
                     /locus_tag="VFH83_13195"
     CDS             5877..6803
                     /locus_tag="VFH83_13195"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6487376.1"
                     /translation="MFRHLLVPLDGSRLAESVLPVAARVARQDGAALTIVHVIERNAP
                     SAVHSDRHLVSADQAATYLEEVARRPGLEGLTVRTHVHTAEVKDVARSITQHTEELAP
                     DLVVMNTHGRGSARRLLFGAIAQQVIGLGQTPVLLLRPGERAEPAPLAAGPWRTILAP
                     VDGDPAHERGLPVAADMALDFHSRLHLLMVVPTMGRLAGFQAAAAMLLPGATRVKLEM
                     EIAGAREYLLQRSEELRHRGVNATGEVTRGDPARAIARAARRLSADLVVMGTHGRAGT
                     DAFWAGSVAARVVARTRTPLLLVPLREEPEPD"
     gene            6877..7728
                     /gene="nadC"
                     /locus_tag="VFH83_13200"
     CDS             6877..7728
                     /gene="nadC"
                     /locus_tag="VFH83_13200"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET6487377.1"
                     /translation="MLSAAPDKTGPGDYLPLVRMALQEDLGDLGDITSQAVVPDGERS
                     ATLWSKDDGVLAGEEIFRSVLREVDPSAVVLFQAHDGARLARGMPVASVRARAISILS
                     AERTALNFISFLSGIATATRKLVDLAEGRAVILDTRKTLPGFRALSKYAVTVGGGSNH
                     RQGLYDMVLIKDNHVDSAGSVAAAVQRVRDRWARRFPVEVECRTEAEVRDAVAAGVDV
                     ILLDNMTTGQMRQAAALVGGRAKVEASGNMTAERIGEVSATGVDYISVGALTHSVASF
                     DFSLKID"
     gene            7742..9310
                     /gene="nadB"
                     /locus_tag="VFH83_13205"
     CDS             7742..9310
                     /gene="nadB"
                     /locus_tag="VFH83_13205"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HET6487378.1"
                     /translation="MEDRREFDLVILGSGIAGLTAALAAAEQGRQVALVTKEPALSEC
                     NTRYAQGGIVARGIDDSAELLANDITVAGDGINSRDAVDLLAAEGPALVDDLLVGKLA
                     VPFTREADGSVGRTREAVHSVRRIYYAKDTTGLAIETSLVAAVEQESRIERFTEHVAI
                     DLITNTHNSTDTQERYRRTRVIGVYLWARSVSMVRIFFAPSVIIATGGVGNLFQHTSN
                     PPGATGDGIAMASRIGAEIINAEYVQFHPTVLHHRDVKRFLISEAVRGEGAKLVNHAG
                     EQFMKRYEPERADLAPRDEVSRAMYREMENEGSEYLYLDARSISEPLPERFPGIFEQC
                     MAVGLDIRKEPIPVVPAAHYFCGGIKVNLQGRASVAGLYAVGEASCTGVHGANRLASV
                     SLLEGLYFGKVAGLDAAKNATPISERLRKSIPSWVNPSQAEDFDPVLIQQDMLSIQST
                     LWNYAGVIRTQKRLVRALADLNYLSHRVEQFYRAAAISRPLVELRNATVVARLIVSAA
                     FSNGKSLGCHFVEP"
     gene            9340..>10047
                     /locus_tag="VFH83_13210"
     CDS             9340..>10047
                     /locus_tag="VFH83_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6487379.1"
                     /translation="MDPTRPVRAAVRITLLCVALALLASCAGLRYRYPGDPQIAALLA
                     GPPPEVPAVRFAVISDAHLYDTGLGTDGSAFQDYLLNDRKLLVQSEEILVAAIQRIRS
                     QDVSFVLITGDLTKDGERQDHLLMARELTALQKAGIKVYVVPGNHDIDNPHAMSYTAK
                     GATPVASILPAEFAENYRDYGYGEAIARDPGSLSYVVEPVPGLWLLAIDSANYATNRQ
                     AGRPVTASGLTQAREDWI"
BASE COUNT         1606 a         3373 c         3352 g         1716 t
ORIGIN      
        1 cgcctcgatg ctcatattgc agagcgtgag ccgagcctcc atgggcagct tctcaatggc
       61 tggacccgag tattcgtagg cgaacccgat cccgccgttc actcccaggt ccgcgatgat
      121 ccggatcacg aggtccttgg cgcccacacc cgacgcgaaa aggccgctca cgtcgatcct
      181 gcggaccttg ggtcgggaca gcgccagggt ctgcgtggca aggatgtccc ggatctgcga
      241 ggtgcccacg ccgaacgcca ggcttccgag cgctccatgg gtggaggtgt gcgagtcccc
      301 gcaggcgatg gtcatgccgg gctgggtcag acccagctcc ggtccgatga tgtggacgat
      361 cccctgctcc gcggagccca ggccgaagaa ggcgatgccg ttggccctgg tgttgtcctc
      421 cacggccttc atcatggctt cggcaagctc gtccgcgaaa ggccgcttct gtccatccgt
      481 agggacgatg tggtccaccg tggcgaacgt ccgattggga aagagcacct tgaggccccg
      541 gtcccgcagc gcctggaacg cttgaggaga ggtgacctca tggacgaggt gcaggccgat
      601 gaaaagctga tcctgcccgg agggcagggt ccccaccttg tgcaggtccc agactttgga
      661 gagaagcgtc ttgcccatgg ctcaggtgac ctcctctccg cgggccatga ggaccttacc
      721 ggtcctcacc atctcgatga ctccgtacgg gttgagcatc cggtgaagcg cgtcgatttt
      781 ctccgtcctt cccgtgacct ggagcgtgat ggcggtctcc gccaggtcca ccacctggca
      841 cttgaaggcg tgcgccacgt ccagcacctc cgtgcgccgg gtggcctcac agcggacctt
      901 gaacagaccg agctcccgct ggatcacgtc ctccgccgtg atgtcacggc catggaccac
      961 gtccaccagc ttgttgagct gcttcaggat ctgctccagg gtcttctcgt ctccgcttgc
     1021 cacgatgttc atgtgagaga acgcggggtc atgggccgga gacgccacga tgctgtcgat
     1081 gttgaaccct cgacgggcga acacctgggc gacccggttg accaccccgg cctggttggc
     1141 cacgtacagg ctcaccaggt ggcgtcggac ggcgcggctg gtggccgggc tcatgatcgg
     1201 accttccgcg gcggctccag gatcatgtcc ttgagcgcgg cccccgccgg gatcatgggg
     1261 aacacattgt cctctttcac cacctcggca tcgatgacca cggggccgtc gttgtagtcc
     1321 agcgcctcct ggagccgccg cgtcacctct gaggccctgc ggatgcgcac ggccttgcag
     1381 ccgtagctct ccgcgagctt cacgaagtcc gggttgcccg ccaggtccac gccggaaagc
     1441 ctgttttcat agaagagatt ctgccactgg cggaccattc cgagataccg gttgttgatg
     1501 atcaggatct tgatggggag cttcaggttc accgcggtgg agagctccgc catggtcatc
     1561 tggaagccgc cgtctcccac gacggccacc accagctctc cggggcgcgc gagctgggcc
     1621 ccgatggcgg cggggaagcc gaaccccatg gtgccggcgc cgccggaaga gatccacttg
     1681 aaccgctcgt ccgtccggta gaactgagcg gcccacatct ggtgctgacc cacgtcggtc
     1741 gtcacgatgg ccttgccgcc ggtcagcttg tagatctcgt cgatgacgtg ctgggccttg
     1801 aagccgccgc ggggattgta cgccagcggg tggaccgtct tccaccggtc gatctgctcc
     1861 agccactgca cggtatcccc ggggaccagg tgctggttca gctccgccag gaccgtccgg
     1921 gcgtctccga cgatggcgca gtccacctgc accaccttgt tgatttccgc gggatcgatg
     1981 tccacgtgga tcttcaccgc atcggtgcag aattggtcga ggtcccccgt gatgcggtca
     2041 tcccaccggg agcccacgga caggatgagg tcacagccgg ccacggcctt gttggcgtag
     2101 gccgtgccgt gcatgcccag catgcccagg ctgaggagat gggtctccgg gaacgcaccc
     2161 ttgccgagca gcgtgctggt caccgggatc cgcatggtct cggcaaaggc tcgaagctcg
     2221 tccccggccc cggagatgag cgcgccgtgc cccaccagga gcaccggtcg cttcgccttg
     2281 gtgagaagct ccgcggccct tcggatctcc accggatccc cttccttggg tacccgatag
     2341 ccgggaagct cgaactcctc ctggtcgtcc acgtcgattc gggtgttcga acaatccttg
     2401 gggatgtcca ccagcacggg accgggccgt ccggtcttgc tgatgtagaa ggcctcccgc
     2461 agcatgcgcg ggagcttggt ggcatccttg acgaggtagg agtgcttcac cacggggaga
     2521 gcgatcccgg agacgtcggc ctcctggaag gtgtccaggc ccaggttcgc gacgctctgc
     2581 tgacccgtga tgaccaccat gggcacggag tccatgtgcg cggtgaggag gccggtgatc
     2641 gtgttcgttg cgccgggacc cgatgtcacc aggacgacgc ccggttcacc cgatgcgcgg
     2701 gcaaacccat ccgccatgtg cgtggcgccc tgctcgtgtc ggaccaggac aagctggatg
     2761 tcggagtcta cgagcgcgtc gaacagcgga atggccgccc cgccgggcag cccgaacacg
     2821 tacttgaccc cctgcttctc caggatatgc accaccgcct gagcgccggt gcaccgcaca
     2881 tcgctccttt tcatctccat ttcttcggga cctcgttgcc ctctaaggta atctccggtc
     2941 cgctccgcgt acagcgcaat cttgaccctg agggtgcaaa gcggtatcaa ggcggaaagc
     3001 tatgccgtac aaggaatggc cgttcatcga ggcacagagg atcgcggacc gggcccggtc
     3061 gatgggaaag agctcggtca cggcggagac cggttacggt ccgtcgggcc tgccgcacat
     3121 cggcaccttc ggcgaggttg cgcgcacctc gttcgtgctg caggcgctgc gcacggtggc
     3181 tccggagatc cagagccggc tcctcgcgtt ctccgacgac atggacgggc tgcgggatgt
     3241 ccccctgaat ctcccgaacg ccgccatgct ccgcgagcac ctgggcaagc cccttaccgc
     3301 catccccgac ccgttcggca aggagaagtc ctttgcccac tacatgaacg gcaagctgcg
     3361 ggagttcctg gactccttcg ggttcgagta ccagttcgcc tcctccacgg agcagtacgc
     3421 ctccggggtg ttcgacgacg gcctgcggag ggtcatggcc aactacgagg ccatcgcgga
     3481 gctcttccga gccaccatcg cggaggagaa gcgggcggcc tggagcccgt tcttcccgat
     3541 ctgcgagagt tgcggccggg tctactccac gcgggtcacc tcgtacgacg cgcaggccct
     3601 gaccgtgagc tacgtgtgtg acgcggccgt gcagggcaag tacggctcct gcggccacaa
     3661 cggcaccacc agcatcctgg ggggccgatg caaggtcggg tggaaggtgg actgggcgct
     3721 gcgctggtac tccctcggca tcgactacga gatgcacggc gaggacctcc tggagtcttc
     3781 ccgcctgtcc tcgcggatcg tgacgatcct gggcggccgg ccgcccgagc tcttcaagta
     3841 cgagctattc ctggacgaga agggcaagaa gatctccaag aggatcggca acgggatctc
     3901 gatcgagcag tggctgaggt tcgcgcccgt ggactgcctc ctctatctca tgttcaccaa
     3961 gcctcagcag gccaagcgga tggggcttcc catccttccg gagatcgtgg atgcctacct
     4021 ggagctggtg gccagcgacg agggctcgga ggattctccc atcccgttca tcacccgtct
     4081 gtccaagggc ggtcatgccg ggaagctcgg ggaccacaag gtcatcacct actccctcat
     4141 cctggagctg atcctggcgc tcaaccagga tgacccggtg atcgcccggg actacctgct
     4201 gaagttccag cccgacattg cgggaaacat ccactactac gagcagctca tcggcaacgc
     4261 gctggcctac ttccgcgagg tgatcgtccc gggtcggacg gcggagaagg cggaccacgc
     4321 ttcggacacg gctatcgccg cgcttcggga tgagctggtc cggctgcggg attccggcgc
     4381 cgcggcaacg gactgggacg cgctgcagac cctggtcttc caggtggcca aggaccggga
     4441 catcaagacc aaggactggt tccgaacgct ctaccgcgtg ttcctggggc agtcctcggg
     4501 tcctcggatc ggcagcttca tcgcccttct ggggtacggc aaatcgatcg agcgcctgga
     4561 ggcgcacctg gcaaccaagg ggtaggtcat ggcgggcaag agcggagacc tgaagaccat
     4621 ccagcaggcc gagagcgtct tggggggaga gaagaagacg gggtggatgg ggcgcgtcct
     4681 tcccttcctg ggccctgctt tcatcgcgtg cgtcgcgtac atcgatcctg gcaatttcgc
     4741 aacgaacgtc caagggggct cggagttcgg tctcatgctc ctgtgggtca tcctggccgc
     4801 caacctcatg gcgatgcttc ttcagagcct ctcggccaag ctggggatcg cgaccggcaa
     4861 gaacctggcc gagcagtgcc gggaacggct gcctggttgg ctggtcgtgg tgatgtggat
     4921 cgtggcggag atcgccgcca tggcgacgga cctcgcggag tttctgggag ccaccttggg
     4981 gttcgccctg ctgttcggca ttcccctgtg gttgggggtg atcctcaccg cgatcgccac
     5041 gttcgtcatc ctgagcctcg agagattcgg atttcggccg ctggaggctg tcatcaccgc
     5101 gctggtgggc gtgatcgccg tgtgctacgt ggtggagatg gggctggtgc ggcccccgtt
     5161 gggtcagatc ctgctgcact ccgtggttcc ccagctctcc ggcacggaga gcgtgctcct
     5221 ggccgtcggc atcatggggg caaccgtgat gccgcacgtg atctacctcc actcctccct
     5281 cacccagagc cgcatcgtgg tcagggaccc caagagactc cgccgcctgt tccgcttcga
     5341 gatcaccgac gtggtgatcg ccatgggcct ggcggggatc atcaacatgg caatgctcat
     5401 catggcgtcg cagaccttcc acgtgcatgg catgacgagc atcgcaacca tcccggacgc
     5461 ccaccgcacg ctggtaccgc tgctgggccc ggccgcgagt tgggtgttcg ctatctcgtt
     5521 gcttgcgtcc ggcctctcgt cgtcctccgt cgggacgatg gcgggccagg tgatcatgca
     5581 gggtttcatc cgccggcgga ttcccgcgtg gatccgaagg ctcgtgacca tggtgccctc
     5641 agtccttgtc atcctcctgg ggatcgatcc cacgcgcgcg ctcgtgctca gccaggtggt
     5701 tctgagcttc gccctgcccg ccccggtcat ccccctcgtc tttttcaccc gacgcaagga
     5761 catcatgggg gtgctggtca acaagcctct gaccacgatc gcggcgggcg cggcagggat
     5821 cgtgatcctc gcgctgaacg tgtacctgct tgttcagacc ttcacaggag ggtgatatgt
     5881 tccggcatct gctggttccc ctggacggtt cgcggctggc agagtcggtg ctaccggtcg
     5941 ccgcgcgggt ggcgcgtcag gacggcgccg ccctcacgat cgtgcacgtg atcgagcgca
     6001 acgcgccgtc cgccgtccac agcgaccgtc acctggtctc cgcggaccag gcggcgacgt
     6061 acctggagga ggtggcgcgg cgacccgggc ttgagggcct caccgtccgc acgcacgtgc
     6121 acacggctga ggtcaaggac gtcgccagaa gcatcactca acacacggag gagctggctc
     6181 cagacctggt ggtgatgaac acccacggcc gcggcagcgc gcggcggctc ctcttcggcg
     6241 ccatcgccca gcaggtcatc ggcctggggc agacgccggt cctgctgctg aggcctggag
     6301 agagagccga gccggcccct ctggccgcgg ggccgtggcg caccatcctg gctccggtgg
     6361 acggagaccc ggcgcacgag cgcggattgc ccgtggcggc ggacatggcg ctggacttcc
     6421 acagccggct ccatctcctg atggtggttc ccaccatggg ccgtctggcg ggcttccagg
     6481 ccgcggcggc catgctcctg cccggcgcca cccgtgtgaa gctggagatg gagatcgcgg
     6541 gggcgcgcga gtacctcctt cagcgctccg aggagcttcg ccaccggggg gtgaacgcga
     6601 ccggcgaagt cacccgaggg gacccggcgc gcgccatcgc ccgcgccgcg cggcgccttt
     6661 cggcggacct cgtcgtcatg ggcacccacg gtcgcgccgg cacggacgca ttctgggccg
     6721 gcagcgtggc cgcgcgcgtg gtggcgcgta cccgcacgcc gctgctgctg gtgccgctgc
     6781 gcgaggagcc ggagccggac tgaggcgccg cgcggaagcc tggaaccggc cttcgcccct
     6841 tgcatacccg caaaccgccg ttctatcctg ctggccatgc tgagcgcggc tcccgacaag
     6901 acgggccctg gggactacct gcctctggtg cgcatggcgc tccaggagga tctgggcgat
     6961 cttggcgata tcacctccca ggcggtggtg ccggacgggg agcgctccgc tacgctgtgg
     7021 agcaaggacg acggcgtcct ggcgggtgag gagatcttcc gttccgtgct ccgcgaggtg
     7081 gacccgtccg ccgtggtgct gttccaggcg cacgacgggg cgcgtctggc ccgcggcatg
     7141 ccggtggcat ccgttcgagc ccgcgccatc tccatcctct ccgccgagcg caccgccctc
     7201 aacttcatct cgttcctctc ggggatcgcc acggcaaccc ggaagctggt ggacctggcc
     7261 gaagggcggg ccgtcatcct ggacacacgc aagacgctgc ccggcttccg cgccctgtcc
     7321 aagtacgccg tgaccgtggg cgggggaagc aaccaccgcc agggcctgta cgacatggtc
     7381 ctcatcaagg acaaccacgt ggacagcgcc ggctcggttg ccgcggcggt tcagagggtc
     7441 cgcgaccgct gggcgcgccg gttccccgtg gaggtggagt gccggacgga ggcagaggtc
     7501 cgggacgccg tcgccgccgg cgtggacgtc atcctgctgg acaacatgac gacggggcag
     7561 atgcgccagg cagcggcgct ggtgggcggc cgcgccaagg tcgaggcgtc cgggaacatg
     7621 accgcggagc ggatcgggga ggtcagcgcc accggcgtgg actacatctc cgtgggcgcg
     7681 ctcacgcact cggtcgcgtc cttcgacttt tcgctgaaga tcgattgagg aggcaccagg
     7741 ggtggaggac cggcgcgagt tcgatctcgt gatcctgggg agcggcatcg ccggcctgac
     7801 ggcggcgctc gccgcggcgg agcagggacg ccaggtcgcc ctggtgacca aggagcccgc
     7861 gctctccgag tgcaacaccc ggtacgccca gggcgggatc gtggcccggg gaatcgacga
     7921 ctccgcggag ctgctggcca acgatatcac cgtggcggga gacgggatca acagccgcga
     7981 cgcggtggac cttctggccg ccgagggccc cgcgctggtg gacgacctgc tggtggggaa
     8041 gctggccgtt cccttcacgc gggaggcgga cggctccgtg ggccgcacgc gggaggcggt
     8101 gcactccgtc cgcaggatct actacgccaa ggacacgacg gggctcgcca tcgagacctc
     8161 cctggtggcg gccgtggaac aggagagccg catcgagcgg ttcaccgagc acgtggccat
     8221 cgacctcatc accaacacgc acaactccac ggatacccag gagcggtacc gccgcacgcg
     8281 cgtcatcggc gtctacctct gggcgcgctc cgtgtccatg gtgcgcatct tcttcgcccc
     8341 ctcggtcatc atcgccaccg gcggcgtcgg gaacctgttc cagcatacct ccaatccgcc
     8401 gggagccacg ggagacggca tcgccatggc cagccggatc ggggcggaga tcatcaacgc
     8461 cgagtatgtc cagttccacc ccacggtgtt gcaccatcgg gacgtcaaac gcttcctcat
     8521 ctcggaggcg gtccgtggcg aaggcgcgaa gctggtcaac cacgcgggtg agcagttcat
     8581 gaagcgctac gagcccgagc gcgccgatct ggcgcctcga gacgaggtct cccgggccat
     8641 gtaccgggag atggagaacg aggggtcgga gtacctctac ctggatgcgc ggagcatctc
     8701 cgagccgctg ccggagcgtt tccccggcat cttcgagcag tgcatggcgg tgggcctgga
     8761 catccgcaag gagcccatcc cggtggtgcc ggccgctcac tacttctgcg gcggcatcaa
     8821 ggtgaacctc caggggaggg cgtccgtggc ggggctgtac gcggtgggag aggcatcctg
     8881 caccggcgtc catggcgcca accggctggc atcggtgtcc ctgctggaag ggctgtactt
     8941 cggcaaggtg gcgggtctgg acgccgccaa gaacgccacc cccatctcgg aacgcctgcg
     9001 caagagcatc ccaagctggg tgaacccgtc tcaggctgag gacttcgatc cggtcctcat
     9061 ccagcaggac atgctcagca tccagtccac cctctggaac tatgccggcg tcatccgcac
     9121 ccagaagcgg ctggttcgag cgctggcgga cctcaactac ctcagccatc gggtggagca
     9181 gttctaccga gcggccgcga tctcgaggcc cctggtggag cttcgcaacg ccacggtggt
     9241 ggcgcgcctg atcgtatcgg ccgccttttc gaacgggaag tccctgggct gtcatttcgt
     9301 cgagccctga agccgggccg acaggacaaa ggagatcgca tggacccgac ccggcccgtc
     9361 cgcgcagccg tccgcatcac gctcctgtgc gtcgccctgg cgctcctggc ttcctgcgcg
     9421 gggctgcgct accgctatcc cggagatccg cagatcgcgg cgctcctcgc cggaccgccg
     9481 ccggaggtcc cggcggtccg cttcgcggtg atcagcgatg cgcacctcta cgacacgggt
     9541 ctgggcaccg acgggagcgc cttccaggac tacctcctca acgaccgcaa gctcctggtt
     9601 cagagcgagg agatcctggt ggccgccatc cagaggatcc gctcgcagga cgtgagcttc
     9661 gtcctgatca cgggggacct gaccaaagac ggagagcgcc aggaccacct tctcatggcc
     9721 cgggagctca ccgccctgca gaaggccggc atcaaggtct acgtggtccc cggcaaccac
     9781 gacatcgaca atccgcacgc catgagctac acggccaagg gcgccacgcc cgtggccagc
     9841 atcctgcccg cggagttcgc ggagaactac cgcgactatg gctacgggga ggccatcgcg
     9901 cgggatccgg gttcgctgag ctacgtggtg gagccggtgc cggggctgtg gcttctggcc
     9961 atcgactccg cgaactacgc aaccaaccgc caggcaggcc gaccggtgac ggcgagcggg
    10021 ctgacccagg cccgcgagga ttggatc
//
LOCUS       DASRZS010000544         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_684426, whole genome shotgun sequence.
ACCESSION   DASRZS010000544 DASRZS010000000
VERSION     DASRZS010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684426"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..2274
                     /locus_tag="VFH83_13215"
     CDS             313..2274
                     /locus_tag="VFH83_13215"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET6487380.1"
                     /translation="MARHREWTERFLSDPPVSFTLDGKAFTGIPASWAPSAKTRRIDA
                     AITERTFAGQDPVSGIAIRIECVEYADFPVTEWTAWFTNTGRAPSPLLSDVQALDALF
                     DGTSPLLWSCNGDFYSGEGYAVQESALGPGETRVFSPSGGRPCDHAFPYFRIVFKGCG
                     LSLAIGWPAQWSASFKGADGSVRVTAGQAKTQLRLMSGETIRTPRITIMSWTGDSVRA
                     ANLWRQWYRAHLLPRSGGRPLGPLVSASGTDEGEEFTAATEANQARFMERWIERGVRF
                     DVWWIDAGWYPCFNTEEKQRRWTMTGTWKPDPERFPRGLGPVSKSAARHGARLLVWFE
                     PERVRPGTALAVEHPDWLLGRAGSPNSLLNLGNPECRRWLTEHICRLVRENGISIYRQ
                     DFNFEPLEHWRTNEPEDRQGINENLHVQGYLQYWDDLLARNPGLWIDSCASGGRRNDL
                     ETLRRSVPLHYTDFGYGDHPVKLSFHHVLFEWIPYFKETTLSWDRSGKARYNSDLDSF
                     AFHCGLGPMIAPAIDIARDDYDFELARRMLVLWRKAAELMIEGDYYPLTPIHRSPDRW
                     VARQFDCPGAGSGFVQAIRLPACPQQALSVQMKAVRDGTTYSFDNPETGEHREMAARD
                     LARAGFTFSLPPRAGALWLYRAIEAAAPG"
BASE COUNT          399 a          791 c          782 g          413 t
ORIGIN      
        1 gtcaggtgac agatgcatgg ttactccggg gaggtccgca ggggcaggcg cagctccccg
       61 atctcccgca tacccgcctt atgtactcct cgatggtcgg ggcgccccag ggcggcgtcc
      121 tatacatggg gtgctgaaga agcgcgacct tccagtccat tcctttctcc ctgcaccatt
      181 atagcagact tggagggggg acgccgggcc gacgttgacg acgcggaaaa cggtgtgcga
      241 gactccgaag ataccgtgat gaagggggaa gctatggaag aaagccgtcg tctctctccg
      301 gatgcagccg acatggcacg gcacagggaa tggacggaac ggttcctttc cgatcctcct
      361 gtttcgttca cgctggacgg gaaggcgttc accgggatcc cggcgagctg ggcgccttcc
      421 gcgaagacgc ggcgcatcga tgcggccatc acggaaagga ccttcgcggg ccaggacccg
      481 gtcagcggga tcgcgatcag gatcgagtgc gtcgaatatg cggacttccc ggtcaccgag
      541 tggaccgcct ggttcaccaa tacgggacgg gcaccgagcc ccctcctcag cgatgtgcag
      601 gccctggatg ctctgttcga cggtacgtcc cctctcctct ggagctgtaa cggtgacttc
      661 tacagcggcg agggatacgc ggtgcaggag tcggcactcg gtccgggcga gacgcgggtg
      721 ttctctccct ccgggggccg gccctgcgac cacgcgtttc cctatttcag aatcgtcttc
      781 aagggatgcg ggctttcgct tgcgatcggg tggccggccc agtggtcggc gtccttcaag
      841 ggcgcggacg gatccgtgcg cgtcacggcg ggccaggcga agacccagct gcgcctgatg
      901 tccggagaga cgatccgcac cccccgcatc accatcatgt cctggacagg cgactccgtg
      961 cgcgccgcga atctctggcg acagtggtac cgcgcccacc tcctgccccg ttcaggcggc
     1021 aggccgctgg gcccgctcgt gtctgcttcg gggaccgacg agggtgaaga gttcacggct
     1081 gccaccgagg cgaaccaggc caggttcatg gagcggtgga tcgagcgtgg agttcgcttc
     1141 gacgtctggt ggatcgacgc cggctggtac ccctgcttca acacggagga gaagcagcgg
     1201 cggtggacga tgacgggcac ctggaagccg gaccccgaac gcttcccccg cggcctcgga
     1261 cctgtgtcga agagcgccgc acgccacggg gcacggctgc tcgtgtggtt cgaaccggaa
     1321 cgggtgcgcc ccggcactgc cctggccgtc gagcacccgg actggctcct gggtcgcgcg
     1381 ggctccccca attccctcct gaacctgggt aatccggagt gcaggcgatg gctgacggag
     1441 cacatctgcc gcctggtccg ggagaacggg atcagcatct atcgccagga cttcaacttc
     1501 gagcccctcg agcactggcg aaccaatgaa ccggaggatc gccaggggat caacgagaac
     1561 ctgcacgtcc agggctacct ccagtactgg gacgacctcc tggcgcgaaa cccggggctg
     1621 tggatcgatt cgtgcgcatc cggtggccgg cgcaacgacc tggaaacgct gcgccgctcg
     1681 gtgcccctgc actacacgga cttcggctac ggggaccacc cggtgaagct ctcgttccac
     1741 cacgtgctgt tcgaatggat cccctacttc aaggagacaa ccctgtcatg ggaccgctcg
     1801 gggaaagccc gttacaactc cgacctggac agctttgcct tccattgcgg gctcggtccg
     1861 atgatcgctc cggccatcga catcgcgcgg gacgactacg actttgagct tgccaggcgc
     1921 atgctcgtcc tctggcggaa ggccgcggag ttgatgatcg aaggcgacta ctaccccctc
     1981 accccgatcc accgctcgcc cgacaggtgg gtcgcgcggc agtttgactg cccgggcgcg
     2041 gggagcgggt tcgtccaggc catccgtctc cctgcgtgtc cgcagcaggc tctcagcgtg
     2101 cagatgaagg ccgtccggga cggcacgacc tacagcttcg acaatcccga gaccggcgag
     2161 cacagggaga tggccgccag ggatctcgcg cgtgccgggt tcactttttc gcttccgcct
     2221 cgcgcgggag ctctctggct ctatcgggca atcgaggcgg cggcaccggg ctgagcaacc
     2281 cgttgccgcg ctcgacacgc tgacggcctc taggaccccg cttggtcctc taggaccccg
     2341 cttggtcctc taggaccccg cttggtcctc taggaccccg cttgg
//
LOCUS       DASRZS010000545         3501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1086723, whole genome shotgun sequence.
ACCESSION   DASRZS010000545 DASRZS010000000
VERSION     DASRZS010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086723"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFH83_13220"
     CDS             <1..470
                     /locus_tag="VFH83_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET6487381.1"
                     /translation="FEMPLAEVVFDFYDRLKSVSRGYASFDYQILGFRETRLSKLDIL
                     LNGKVVDALAQLVFRDNAYQTARRVCERLRDEIPRHQFKIAIQGAIGSQIIARETVNA
                     FRKDVTAKCYGGDITRKRKLLEKQKEGKRRMKMVGEVELPQSAFLAVLKTSEE"
     gene            complement(432..2093)
                     /locus_tag="VFH83_13225"
     CDS             complement(432..2093)
                     /locus_tag="VFH83_13225"
                     /inference="COORDINATES: protein motif:HMM:NF014075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HET6487382.1"
                     /translation="MPGEGPRLLNGERGSTLLWRDLAFYPPSDPVSSARRKARGFPIS
                     SRTLVFVPSIGLGYGLAELLGRLPEQCAVVCAEVFPEVMAEAIRVGLPRDPRIVVVRT
                     EDPEALVAAVRAMGVGRFRRVVEAPLCAGYRLARQTYERMRQAIAQELAGYWRNRLTL
                     TALGSRMVCNLLDNLTLLPEAGDIGDLGTELPVVVVGAGPSLDSTLEPLAPLRDRFAL
                     VAVDTAVPALAARSIVPDLIVALEAQASNLQDFIPARDPAPLLACELASHPSVARLYR
                     GRLFFFSSAFAPLPIFDRLAGAGILPTPIPGLGSVGVCAAYLALRLTRGDVYLTGLDF
                     SFPGGRTHARGTPYHLAMLHRCERVKPIGQLAYQAMAARDRIHVRGKDGASLQTDGVL
                     RSYRDTLRRETAAEARRIIDIGDVGMDLDLRAVSVTEAAGRLKKSARGSGFGVSGRGS
                     ADASRRERFDGRRIQEFLRAERDLLVRASEAARQAASNRIVDPACAELIRAVQHAWVH
                     FPDDPDPAEAGPSFLARASVASGYYANRADRLCSLLGGLEDGQEG"
     gene            complement(2100..>3501)
                     /locus_tag="VFH83_13230"
     CDS             complement(2100..>3501)
                     /locus_tag="VFH83_13230"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6487383.1"
                     /translation="GSPVASRIQGSGAIVFGSYAGKDISYYQGSYFAQDLSRLNSQRN
                     TQGTASSADLQSIYYQAFIDTAEHIAILDQVDRAGGQVSNDAVDKSLLTYSGYLDDNG
                     KFSEARYNATSGSERASTRKLYREDLLTNQFITDIVYGVKDGSKESAFIKDMVAPERK
                     FAFVSYPFSGFPSDQVKAYGEANKSRFRKIKVSRILVKSSESQADEILKKIKDKASTF
                     EELAKTYSKDGYADKGGDMGWRYAYDLEADFDAKDTAQKIFALKAGEVSDVLKGTFGW
                     MIYRCDADATDPDFSDPAVQTDVRGYITQYEKGKIEDYFTSQASVLARRAADVGFDKA
                     AAEAKLKVLQTAAFPVNLGNFFSFAPFQAMNQADTPSSAVYNEDFFYRAFSLGKDQTS
                     QPVVLDDQVLVLKLLEESRLPDSTLNLLGSWITYIASQSVQRDLSAVLMTPDKLKDNF
                     VGTFNRYFTARTSTQQ"
BASE COUNT          600 a         1127 c         1187 g          587 t
ORIGIN      
        1 ttttcgagat gccactcgcc gaagtcgtct tcgacttcta cgatcggctg aagtcggtga
       61 gccgtggata cgcatccttc gactatcaga tcctggggtt ccgcgagacc cgcctgtcca
      121 agctggatat cctcctgaac gggaaggtgg tggacgccct ggcccagctc gtgttccgcg
      181 acaacgccta ccagactgcc cgacgggtct gcgagcggct tcgcgacgag atcccgcgcc
      241 accagttcaa gatcgcgatc cagggggcaa tcgggagtca gatcatcgcg cgggaaaccg
      301 tcaacgcgtt ccgaaaggac gtcaccgcga agtgttacgg gggagacatt acccgcaagc
      361 gcaagctcct ggagaagcag aaagagggca agcggcggat gaaaatggtg ggggaggtgg
      421 agctgcccca atcagccttc ctggccgtcc tcaagacctc cgaggagtga gcacagccgg
      481 tccgcccggt tggcatagta gcctgacgcc accgaggcgc gggcaaggaa gctgggcccc
      541 gcctccgcgg ggtccgggtc atcggggaag tggacccacg cgtgctgcac cgctcggatc
      601 agctcagcgc acgccgggtc gacgatgcgg ttcgaggcag cctgccgggc cgcctcggac
      661 gccctgacca ggaggtcccg ttcggcgcgg aggaactcct ggatccggcg tccgtcgaac
      721 ctttcacggc gagaggcgtc ggcagaccca cgccccgata ccccgaaccc ggagccacgg
      781 gcgctcttct tgagtcgacc ggccgcctcc gtcaccgaca ccgctcgcag gtcgaggtcc
      841 atgccgacat ctccgatgtc gatgatgcgc cgggcttcgg cggccgtctc ccgtctgagc
      901 gtgtcccgat acgagcgcag cacgccatcc gtctggaggg aggcgccgtc cttgccccgg
      961 acgtggatac ggtcgcgcgc cgccatcgcc tggtaggcaa gctggccgat gggtttgacc
     1021 cgctcgcacc ggtggagcat ggccaggtgg tacggcgtgc ctctggcgtg ggttcggccc
     1081 ccggggaagc tgaagtccag accggtgagg tagacatcgc cccgcgtcag gcgaagagcc
     1141 aggtacgccg cgcatacgcc taccgaaccg agccccggaa tcggcgtggg caggatcccc
     1201 gcaccggcga ggcggtcgaa gatcggcagc ggagcaaagg cggagctgaa gaagaagaga
     1261 cgtccgcggt agagacgcgc aacggaagga tgggaagcca gctcgcacgc caggagcggc
     1321 gcggggtccc gggcgggaat gaagtcctga aggttggatg cctgcgcttc cagggctacg
     1381 atgaggtccg gcacgatgga gcgcgcggcc agggcgggga ccgccgtgtc cacggccacc
     1441 agcgcgaaac gatctctcag gggagcgagc ggctccaggg tggagtccag ggacggcccg
     1501 gcgcccacca ccaccacggg cagctcggtc cccaggtccc cgatgtcgcc cgcctcgggc
     1561 agcagggtca ggttgtccag gaggttgcag accatgcggc tgcccaacgc cgtaagcgtg
     1621 agccgattgc gccagtatcc ggccagctcc tgcgcgatgg cctgtcgcat ccgctcatag
     1681 gtctggcggg ccagccgata ccccgcgcac aacggcgcct cgacgacgcg tcggaaccgg
     1741 cccacgccca tcgcgcgaac ggcggccacc agcgcctcgg gatcctccgt cctcacgacg
     1801 acgatcctcg ggtcccgtgg caggccgacg cggatcgctt cggccatcac ctctggaaag
     1861 acctccgcgc acaccacggc gcattgctcg gggagtcgtc ccaagagctc tgcgaggccg
     1921 tagccgagcc cgatggacgg aacgaagacg agcgtcctcg acgagatggg aaagccgcgc
     1981 gcctttcggc gcgcggagct cacgggatcc gagggtgggt agaaggcgag gtctcgccag
     2041 aggagggttg agccgcgctc accgttcagc aggcgggggc cctcgcccgg catgaagccc
     2101 tactgctggg tcgaagtgcg cgccgtgaag taccggttga acgtccccac gaagttgtcc
     2161 ttcagcttgt ccggcgtcat caggacggcg ctgagatctc tttggacgga ctggctggcg
     2221 atgtaggtga tccagctacc cagcaggttg agcgtggagt ccggcagcct gctctcctcc
     2281 agcagcttga gcaccagcac ctgatcgtcc agcactaccg gttgcgacgt ctggtccttt
     2341 ccgagggaga aggcgcggta gaagaaatcc tcgttgtaca cggcgctgct cggtgtgtcc
     2401 gcctggttca tggcctggaa gggggcgaac gagaagaagt tcccgaggtt caccgggaag
     2461 gccgcggtct ggagaacctt cagcttggcc tcggccgccg ccttgtcgaa gccgacgtcc
     2521 gccgcccgcc gggccaggac gctggcctga gaggtgaagt agtcctcgat cttgcccttc
     2581 tcgtactggg tgatgtagcc gcggacgtcg gtctggaccg cggggtcgct gaagtcggga
     2641 tcggtggcat ccgcgtcaca gcggtagatc atccacccga aggtgccctt gaggacgtcg
     2701 ctcacctctc ccgccttgag ggcgaagatc ttctgggccg tgtccttggc gtcgaaatcc
     2761 gcctccaggt cgtacgcgta gcgccatccc atgtcgccgc ccttgtccgc gtagccgtcc
     2821 ttggagtagg tcttggcgag ctcctcgaag gtgcttgcct tgtccttgat cttcttcagg
     2881 atctcgtccg cctggctctc ggagctcttc acgaggatcc gactcacctt gatcttgcgg
     2941 aaacgggact tgttggcctc accgtaggcc ttcacctggt ccgaaggaaa gccggagaac
     3001 gggtaggaga caaaggcaaa cttgcgttcg ggggccacca tgtccttgat gaacgcggac
     3061 tccttggagc cgtccttcac gccatagaca atgtccgtga tgaactggtt ggtgaggagg
     3121 tcctcccgat acagcttccg cgtggaggcg cgctccgagc ccgaggtggc gttgtaccgg
     3181 gcctcgctga acttgccgtt gtcgtccagg tacccggagt acgtcaggag ggacttgtct
     3241 accgcgtcgt tggagacctg cccccctgcc cggtccacct ggtccaggat ggcgatgtgc
     3301 tcggccgtgt cgatgaacgc ctggtagtag atggactgca ggtccgcgct gctggcggtg
     3361 ccctgggtgt tgcgctgcga gttcaggcgg ctcagatcct gggcgaagta gctgccctgg
     3421 taataggaaa tgtccttccc cgcataggag ccgaacacga tggccccgct cccctggatc
     3481 cggctggcga ccggactgcc g
//
LOCUS       DASRZS010000546         4224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1846533, whole genome shotgun sequence.
ACCESSION   DASRZS010000546 DASRZS010000000
VERSION     DASRZS010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846533"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..228)
                     /locus_tag="VFH83_13235"
     tRNA            complement(157..228)
                     /locus_tag="VFH83_13235"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(194..196),aa:Val,seq:cac)
     gene            699..1229
                     /locus_tag="VFH83_13240"
     CDS             699..1229
                     /locus_tag="VFH83_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET6487384.1"
                     /translation="MAFVPLLSSINLPGTVALLGVGTLFYTFAELSRRNGNTILVVSN
                     LTLLASRDRDRGRFVLGPITLALGAMISLLLYPEPAASIAIYALAFGDGLASLVGTVI
                     RGPQIPLLKGKTLSGSSACFIAVFAVTFKITQRPVESLVVAGSATVLEGIPTGNFDNL
                     MVPVGVGLVASRLLLG"
     gene            complement(1281..2138)
                     /locus_tag="VFH83_13245"
     CDS             complement(1281..2138)
                     /locus_tag="VFH83_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487385.1"
                     /translation="MRAAPGTIAFTTAAALTLALAIGAGVVLAMLLLVPGFADPLRTL
                     PDNVWWFTYRDAPENGSAGALWRVGAAIVVCLAVLPAAVRARRLQRQSSSSLPVFLTL
                     FFFTLSVEGLRAASALLYARDGSIQLSIVCTRTVYGGRIAGLLSLLIAGLYCIEWRYR
                     RHGILATVVLVTAFAVATAIPVDRTEFLAQLTWKLGDEQGIWFVNLIIGLLALATVAS
                     ATWVRHDRRYLWLTLGFALLLAGRELFCFAVSPGPTAGGVAMIAIGGLVCLRTLSALY
                     RPVVEPASR"
     gene            2193..3209
                     /gene="aroF"
                     /locus_tag="VFH83_13250"
     CDS             2193..3209
                     /gene="aroF"
                     /locus_tag="VFH83_13250"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET6487386.1"
                     /translation="MILVFRPDATEAQIQSALAKIQSLGYTPHLSRGTVKTICGVTGD
                     DRSIEESEFDQLPGVESVLRVLKPYKLASREFHPDPTVVDVGGVPVGEGSFVVIAGPC
                     SVEDEAQLESAAAFVAGLGLKLIRGGAFKPRSSPYSFQGLGEQGLEILCSAARRHGLT
                     VVTEILSEHDVELVGRYTGVFQVGARNMQNFALLKALGETRRPVLLKRSMMGTIEELL
                     LSAEYIMKGGNSRVILCERGIRTFETETRNTLDIGAVPAIKRLSHLPILVDPSHAMGD
                     WHYVPAASLAALAAGADGLLIEIHPHPAQAKSDGRQSLNYPRFTALLERMRALASCLG
                     VSVR"
     gene            3283..3975
                     /locus_tag="VFH83_13255"
     CDS             3283..3975
                     /locus_tag="VFH83_13255"
                     /inference="COORDINATES: protein motif:HMM:NF021529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2259 domain-containing protein"
                     /protein_id="HET6487387.1"
                     /translation="MAKRLSLCLAVFLVATIVAGAGDVAQFVNLGFSADSKYFMFGQY
                     GVLQKTSTPWAEAYIVNVVGNAFAPHGARRFSTTRALEPGASALGGLLTALADIQPQV
                     KQYRIDHLLPGRLLYILVDGQQPPDTLQFRDFQSGGSYTIDLRQSSTTNTNEVRSSFG
                     LTVTVTQKDGATRTLTAGSPTLERLGVKAYHVKEILLAPDNASLVFLIQRQEQDTDGD
                     NIRYMVETLKMK"
     gene            3995..>4224
                     /locus_tag="VFH83_13260"
     CDS             3995..>4224
                     /locus_tag="VFH83_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6487388.1"
                     /translation="MTISVSSLSVSFQKRPALRDVSLEVPSARIVGLLGPSGAGKTTL
                     IRCIAGLLIPHAGLVRIDSRRIPDRGISRSVG"
BASE COUNT          788 a         1375 c         1351 g          710 t
ORIGIN      
        1 agatggcggc gacggaaccc ccgcagcgcg cgatccgccc ggatcccgag ccaagccaac
       61 ccgggcgcac caaagttggg cgcaggacgc ctagaacaac agggatcagg cctgggaggt
      121 gtcagcggga actatccccc ctcaagagaa gagctttggg cgatactgga cttgaaccag
      181 tgactcctac cgtgtgaagg tagtactcta ccgctgagtt aatcgcccat cgaaggctgg
      241 gcccagcggg ccactgccca cgatggggcg tactgtacaa cggcgcttag aatggtgtca
      301 atggcggaac tgggcgcctg ggcgccactg ggaagcaagc gagggaacgg gccggtcgga
      361 gagcggcgga gtacccatcg tccgttgcgt ttggatgaac ctgagcggaa tgtatgtata
      421 tgaaaatcaa aatatccgtt gcatgaaagt agacaggaat ggagatttga ccggccaagg
      481 tctatcgccg acacctaagt tcttactgac aagaacatta tagtaggcgg gaaacggggt
      541 gggttggtat cacacttgca ttgactaagg caacgaatgc tccaatgacg taccaacaac
      601 cggatcctgc aggacgcttt gaagttcagc gagttcgatc ggcaacgcag cctacgggga
      661 ttctgggaga ggccatcagg aagtctctcc atctcctggt ggcgtttgtg cctctgcttt
      721 cttccattaa tctacccggc accgtagcgc tgctcggggt ggggacgctc ttctacacct
      781 tcgcggagct gtcccgcagg aacggcaaca ccattctcgt ggtcagcaac ctcacgcttc
      841 tggcctccag ggaccgggac cgcgggcgtt tcgtgctggg tcccatcact ctggcgctcg
      901 gagcgatgat ctcgctcctg ctctacccgg agcccgccgc ttccatcgcc atctacgcat
      961 tggcctttgg tgacggcctg gccagcctgg tcggcaccgt gattcgcggt cctcagattc
     1021 cgctgctcaa gggcaagacg ctatccggca gcagcgcctg cttcattgcc gtcttcgccg
     1081 tgaccttcaa gatcacccag cgtccggtgg agtcccttgt cgtggccggc agcgcgacgg
     1141 tcctggaggg gatacccacg ggcaacttcg acaacctcat ggtacccgtc ggggttggac
     1201 tggtagcgtc ccgcctgttg ttgggctgag ccgaccgctc ccgagcccgc tgattctgac
     1261 gcccgaaccc tgatcccctc tcaccgagac gccggttcga ccaccggtcg gtagagggcg
     1321 gagagcgtcc gcaagcagac caacccgccg atcgcgatca tcgccacccc accagcggtg
     1381 gggccggggc tcaccgcgaa gcagaaaagc tcccgccccg ccagcaggag cgcgaagccg
     1441 agtgtgagcc acagataccg acggtcatgt cggacccacg tggcgctcgc caccgtggcg
     1501 agggccagaa gcccgatgat caggttcacg aaccagattc cctgctcgtc gccgagcttc
     1561 caggtgagct gggccaggaa ctccgttcgg tcgacgggga tggccgtagc gaccgcgaac
     1621 gccgtcacca ggacgaccgt cgccaggatg ccgtgacggc gatagcgcca ctcgatgcaa
     1681 tacagcccgg cgatgaggag cgacaagagg ccggcgattc gtccgccgta gacggtcctc
     1741 gtgcagacga tggagagctg gatggacccg tctcgggcgt agagcaaggc gctggcggcg
     1801 cgcaggccct ccacgctcag ggtgaagaag aacagcgtca ggaatacggg aagggaagag
     1861 gagctctgcc gttggaggcg tcgggcgcgt accgccgccg gcagcaccgc aaggcagacg
     1921 acgatggcag cgccgacccg ccataacgca ccggcgcttc cgttttccgg ggcgtcgcgg
     1981 taggtgaacc accacacgtt gtccggcagg gtccgaagcg gatccgcgaa gccggggacc
     2041 agcaggagca tggccagcac cacgccggcg ccgatggcca gcgccagggt gagcgcggcc
     2101 gccgtagtga aggcgatggt gcccggggcg gctctcatgg cgcaattgta cgccgatatg
     2161 tgcgaaaagg tcaaaacata gtagcctccg tcatgatcct cgtcttccga ccggacgcca
     2221 cggaggcgca gatccagagc gccctggcca agattcaatc tctgggctac accccgcatc
     2281 tcagccgcgg aacggtgaag acgatctgcg gggtcacggg ggacgatcgt tccatcgagg
     2341 aatccgagtt cgaccagctc ccgggcgtgg agagcgtgct gcgggtcctg aaaccctaca
     2401 agcttgcctc gagggagttc cacccggacc cgacggtggt ggacgttggg ggcgtccccg
     2461 tcggggaagg gtcgtttgtc gtgattgccg ggccgtgctc ggtagaggac gaggcgcagc
     2521 ttgagtccgc ggcggcgttc gtggccgggc tcgggctgaa gctcatccga gggggcgcgt
     2581 tcaagccgcg ttcatcgccg tactccttcc agggactggg agagcagggg ctggagatcc
     2641 tgtgctccgc ggcccgccgc cacgggctca ccgtggtgac cgagatcctt tccgagcacg
     2701 acgtcgagct ggttggacga tacaccggcg tcttccaggt gggcgcgcgc aacatgcaga
     2761 acttcgcgct cctcaaggct ctcggggaaa ctcgaagacc cgtgctcctc aagcgcagca
     2821 tgatgggcac catcgaggag ttgctcctct ccgcggagta catcatgaag ggcggcaact
     2881 cccgggtgat cctgtgtgag cgcggcatcc gcaccttcga gacggagacc cgcaacaccc
     2941 tggacatcgg cgccgtgccc gccatcaaga ggctctccca cctgcccatc ctggtggacc
     3001 cgagccacgc catgggagac tggcactacg tgcccgcggc gtccctggcg gccctcgccg
     3061 cgggagccga cgggctcctg atcgagatcc atccccatcc cgcccaggcg aagtccgatg
     3121 gacggcagag cctgaactac ccacggttca ccgcgctctt ggagcggatg cgggcgctgg
     3181 cctcctgcct gggcgtcagc gtgcgttagg cggggcatct ggccgccgca aatacttcta
     3241 tagaaaactc cctcggatac gatactagaa caaggagtac ctatggctaa acggctctcc
     3301 ttgtgcctgg ccgtcttcct cgttgccacg atcgtcgccg gcgccgggga cgtcgcgcag
     3361 ttcgtcaacc tcggattctc cgccgactcg aaatacttca tgttcggtca gtacggcgtg
     3421 ctccagaaga cctccacccc gtgggcggag gcgtatatcg tcaacgtcgt cggcaacgcg
     3481 ttcgcgccgc acggggcgcg gagattctcc accacgcggg ccttggagcc cggcgcaagc
     3541 gcgctgggtg ggctcctcac ggccctggcg gacattcagc cccaggtcaa gcagtaccgc
     3601 atcgatcacc tgctgccggg ccggctcctc tacatcctgg tggatggcca gcagccgccg
     3661 gacacgctgc agttccgcga cttccaatcc ggcggctcat acaccatcga cctccgccag
     3721 agctccacga cgaataccaa cgaggtccga tcctccttcg ggctcacggt cacggtcacc
     3781 cagaaggacg gcgccacgcg gaccctgacg gccggctctc ccaccctgga gcggctgggg
     3841 gtgaaggcgt accacgtgaa ggagattctc ctggcgccgg acaacgcgtc cctggtgttc
     3901 ctgatccagc ggcaggagca ggacacggac ggagacaaca tccgctacat ggtcgaaacc
     3961 ctgaagatga agtagccgcg atcggcggaa ccccatgacc atctccgtct ccagcctctc
     4021 ggtgagcttt cagaagagac ccgccctgcg cgacgtatcg ctggaggtgc cctccgcgcg
     4081 gatcgtcgga ttgctgggtc cttcgggcgc cggcaagacc accctgatcc gctgcatcgc
     4141 cggactgctc atccctcacg cggggctggt tcgcatcgat tcgcgccgca ttcctgaccg
     4201 cggcatctcg cgctccgtcg ggta
//
LOCUS       DASRZS010000547         3210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3372194, whole genome shotgun sequence.
ACCESSION   DASRZS010000547 DASRZS010000000
VERSION     DASRZS010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3372194"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFH83_13265"
     CDS             complement(<1..801)
                     /locus_tag="VFH83_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6487389.1"
                     /translation="MKLRVGVAVLILALLVVPVALFAAGTKEAGAKGAPIATFKYWVP
                     IAEFVATRSSNLGDIPMYKELEKRTNVHIVFSHPPAGQEQDQFNLMIASRDLPDIIEW
                     SWISYPGGPEKAISDKIVIPLNDLIKKNAPNLTKLIGLDKERDKLFKTDNGTYYAYPF
                     IKSPLENGFTLVWHGPQFRKEWLDELKLPVPTTMDEWESTLMAFKTKKNADAPLSFNW
                     LNLPISNSFVSAYGVGIDYYQENGKIKFGPIEPGFKDFLTTFNRWYNEG"
     gene            947..3019
                     /locus_tag="VFH83_13270"
     CDS             947..3019
                     /locus_tag="VFH83_13270"
                     /inference="COORDINATES: protein motif:HMM:NF027792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5054 domain-containing protein"
                     /protein_id="HET6487390.1"
                     /translation="MAPTMHVVFKTHLDVGFTHFAAVVKRRYFDHFIPTALAIARELR
                     QEGGEDRFVWTTGSWILYEYLEQADPAGRKAAEQGILAGDLRWHGLPFTVHSELMDQD
                     LFRYGLSLSGDLDRRFGVHTVAAKMTDVPGHTRGVVPLMAEAGLKFLHIGVNHASSAP
                     GVPDVFRWREPDSHAEIMVMYHKAAYGSVTTVEGLDHALAFAHTGDNQGPQSKTQIVE
                     ELARLRAEYPGYQVRASTLDAFAQELDAVRDRLPVVEQEIGDTWIYGTASYPSMLAEF
                     RELQRLRSRWLRAGGAAVRDPAFERFSRELLSVPEHTWGLDQKMYLADYRNYPRKDFE
                     RARERDAVVDGIPEEFPFARGFAHDGVMQSYRLMEASWLEHRAYLARAREALRGTPFG
                     AEAERAAEACRPVRPSAERSVPASALEKGFDLRHFGVRISPANGALVSLVEKKTGRDW
                     ASPAHPLGLFCYQTFSSGDYDRYYQEYVRNLDEPGTYAWAAPDYGRLGLRPEHSESAV
                     SKPKVVRVRRSADTFVVDLLLPVKPRSRYGAPAEVAVCYRFPRQAPTVEVELTWFGKP
                     ANRMPEASWFSFSPVIADPAGWEMEKLGHWVSPLDVVGHGNRYLHGVGEGVRIQDGAS
                     RLVIQSQDAHLVSPGEPRALRFDDALPPLAGGMHFCLHANLFGTNFPLWYDEDGRFRF
                     RLRFERAP"
     gene            complement(3028..>3210)
                     /locus_tag="VFH83_13275"
     CDS             complement(3028..>3210)
                     /locus_tag="VFH83_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HET6487391.1"
                     /translation="AVDLAPAWPLKSPVSLSSIKSEPSLKDILLVRVGRLSVLPLEKE
                     QFETIVSMGGGKAKLA"
BASE COUNT          514 a         1023 c         1073 g          600 t
ORIGIN      
        1 gccctcgttg taccagcggt tgaacgtggt caggaagtcc ttgaaccccg gctcgatggg
       61 tccgaacttg atcttcccgt tctcctggta gtagtcgatc cccaccccat acgcgctcac
      121 gaaggagttg gagatgggca ggttgagcca gttgaaggag agcggggcat ccgcgttctt
      181 cttggtcttg aacgccatga gggtggactc ccactcgtcc atcgtggtgg ggacggggag
      241 cttcagctca tccagccact ccttgcggaa ctggggcccg tgccatacca gggtgaatcc
      301 attctccaac gggctcttga tgaacggata cgcgtagtag gtcccgttgt ccgtcttgaa
      361 gagcttgtcc cgctccttgt ccagcccgat gagcttggtc agattcggcg cgttcttctt
      421 gatcaggtca ttcagaggga tgacgatctt gtcgctgatg gccttctccg ggccgcccgg
      481 atagctgatc cagctccact cgatgatgtc cggcagatcc cgggatgcga tcatcaggtt
      541 gaactggtcc tgctcctggc ccgcgggggg atggctgaac acgatgtgca cgttcgtccg
      601 cttctccagc tccttgtaca tcgggatgtc tcccaggttg ctggaacggg ttgccacgaa
      661 ctccgcgatg ggaacccagt acttgaacgt ggcgatcggc gcgcctttgg cgccggcctc
      721 cttggtcccc gcggcgaaca gcgcaacggg caccaccagg agcgccagaa tgaggacggc
      781 aacacccact ctgagcttca tacggcctcc ctttccatgg ctcgatttcc gataggctac
      841 acgatcctgg ccggaagtca agcgaaatgt tgtctcccac gtgtgtttac gatacgcttc
      901 tgcctgtttc cgatacctcg caggtttccg acgaaggagc atcgccgtgg cgccgaccat
      961 gcatgtcgtg ttcaagaccc acctggacgt agggttcacg cacttcgcgg ccgtcgtgaa
     1021 gcgccgctac ttcgatcact tcatcccgac cgcgctcgcc attgcgcgcg agctccgcca
     1081 ggagggcggg gaggaccggt tcgtgtggac cacgggctcc tggatcctct acgagtatct
     1141 ggagcaggcc gatcccgctg gccggaaagc ggcggagcaa gggatccttg cgggcgatct
     1201 gcgctggcac ggacttccgt tcaccgtcca cagcgagctg atggaccagg atctgttccg
     1261 ctatggcttg agcctctccg gggacctgga ccggcgcttc ggagtgcaca cggtggcggc
     1321 caagatgacc gacgtccccg ggcacacccg cggtgtggtt cccctgatgg cggaggccgg
     1381 tctgaagttc ctccacatcg gcgtgaacca cgcgtccagc gcgcctgggg tcccggacgt
     1441 ctttcggtgg cgggagccgg actcccacgc ggagatcatg gtcatgtatc acaaggcggc
     1501 ctacgggagt gtcaccacgg tggagggcct ggaccacgcc ctggccttcg cgcataccgg
     1561 agacaaccag ggcccgcagt ccaagacgca gatcgtggag gagcttgccc ggcttcgcgc
     1621 cgagtatccg gggtaccagg tccgtgcctc gacactggac gcctttgccc aggaactgga
     1681 cgcggtgcgg gaccgcttgc ccgtggtgga gcaggagatc ggggatacgt ggatctacgg
     1741 caccgccagc tacccgagca tgctcgcgga gttccgggag ctccagcggc tgcggagccg
     1801 gtggctgagg gcgggcgggg cggcggtccg cgacccggcc ttcgaacggt tcagccggga
     1861 gcttctctcc gtaccggagc acacctgggg tctggaccag aagatgtacc tggcggacta
     1921 ccgaaactat ccgcgcaagg atttcgagcg ggcccgcgaa cgggacgccg tggtggacgg
     1981 gatcccggag gagtttccct ttgcccgcgg ctttgcccat gatggtgtga tgcagagcta
     2041 ccgcctgatg gaggcctcgt ggctggagca tcgggcctac ctggcccggg cgcgggaagc
     2101 gcttcggggg acaccgttcg gcgcggaggc ggaacgcgcg gcggaggcgt gccgtcccgt
     2161 ccgaccttcg gcggaacggt ccgtgcccgc ctcggcgctg gagaagggct tcgaccttcg
     2221 ccacttcggg gtgcgcatca gccccgccaa cggagcgctt gtctccctgg tggagaagaa
     2281 gaccggcagg gactgggcgt cccccgcgca tcccctcggg ctcttctgct atcagacctt
     2341 ctccagcggt gattacgatc gctactatca ggagtacgtt cgcaacctcg acgagccggg
     2401 cacctacgcc tgggctgcgc cggactatgg gaggctgggg ctgaggcccg aacattcgga
     2461 gtccgccgtt tccaagccca aggtcgtgcg cgttcgacgg agcgcggaca ccttcgtggt
     2521 ggacctcctg ctgcccgtca agcctcggtc ccggtacggc gcgcccgcgg aggtagcggt
     2581 gtgttaccgc ttcccgcgcc aggcgccgac cgtcgaggtc gagctcacgt ggttcggcaa
     2641 gccggcgaac cggatgccgg aggcttcgtg gttctccttc tcaccggtta tcgcggatcc
     2701 cgcgggatgg gagatggaga agttgggtca ctgggtctct cccctggacg tcgtcggcca
     2761 cgggaaccgg tacctgcacg gagtggggga gggtgtgcgg atccaggacg gggcctcgcg
     2821 gttggtcatc caaagccagg acgcgcacct ggtgtccccc ggagagccgc gcgcgctccg
     2881 gttcgatgac gctctgccgc cgctcgccgg cgggatgcac ttctgcctcc acgccaacct
     2941 cttcgggacg aacttcccgc tctggtacga cgaggacggg cggtttcggt tccggttgcg
     3001 cttcgagcgc gccccgtaga ggtgccccta cgccagcttc gctttcccgc cgcccatgga
     3061 gacgatcgtc tcgaactgct ccttctccag aggcagaacg gacagtcgtc caacgcgcac
     3121 caggaggatg tccttgagcg aaggctcgga cttgatcgag gagagagaca ccgggctctt
     3181 gaggggccag gccggagcga ggtccacggc
//
LOCUS       DASRZS010000548         5375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_685691, whole genome shotgun sequence.
ACCESSION   DASRZS010000548 DASRZS010000000
VERSION     DASRZS010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685691"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VFH83_13280"
     CDS             complement(<1..672)
                     /locus_tag="VFH83_13280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HET6487392.1"
                     /translation="MTSSPGKPAPSGRSEASELPRVVILGRPNVGKSTLFNALLHRRR
                     AITHSRAGVTRDPVEVDCALGGKRVTLVDTGGFAADADGVDAQVSVRSLRAAEQADLI
                     LMILDALETTAADDDFLERLRPYSPKVVLVVNKVDTPERDPMVWNAHAYGFSHVLGVS
                     AAHGRGLGELADLAAQLLEAGGEDRRGAKGGALRSPQGGALRSPQGGALRSPQGGALR
                     SPQGGA"
     gene            complement(653..2374)
                     /gene="ptsP"
                     /locus_tag="VFH83_13285"
     CDS             complement(653..2374)
                     /gene="ptsP"
                     /locus_tag="VFH83_13285"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET6487393.1"
                     /translation="MMEFTGISASPGIARGKALLVLEDKLTVPRYDIQASQVEFENER
                     FLVALGQAAEEVRGLATGRDAALFEAQLLMFEDPELKSQVRAELERAHHNVEWTLLSI
                     LEQLSAKLEAAESQYLRERSVDLSDAGQRIISQLLHRKRARLSDLSQPVILITHNLMP
                     SDTLGLRRGIVLGIATDVGGKTSHTAILARSHEIPAVLGLSDISRQVTNDAEVIVDGN
                     RGVVIVHPDAPTREVYNRRRKDWERKYVVLKGLKELPAETLDGKRIAIEANIEFPEEA
                     PAVLAHGADGVGLFRSEFLFLQPNRFPSEDEQYQAYTLALTSMAGRNVTIRTLDLGGD
                     KLMPGFQIDQDGNPLLGWRAIRFCLARPEIMRTQLRALLRSSVHGALRIMFPLVSGAG
                     ELERIQVLLAEVKADLLREGVAFRDDVAVGIMIEVPSAAVTSDILAQKADFFSIGTND
                     LIQYTLAVDRGNEKVAYLYEPFHPGVLRLIRTTIGNAHARGIPVGMCGEMAGDPLATV
                     VLLGMGLDSFSMGPIGVPLIKRIIRSVRMSDAEALARRVMPLGSGAEIESAVRGWMGE
                     RLDFFSW"
     gene            2434..3327
                     /locus_tag="VFH83_13290"
     CDS             2434..3327
                     /locus_tag="VFH83_13290"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HET6487394.1"
                     /translation="MAATRTSRKKAQASNRRTLGCLFWVCLVLVIAAVGYAARVPLQD
                     AFGRLVSTLGGKHGGPVTPPQVTVSPISQPQGQTAPATNPPRPTKQAPIPSGQSPGTA
                     PANPPAQKTQSGPGQQPTQPAQGGQPAQQPPQQAPGAQPAQQAPVTQQKAQARKARLY
                     FVAVDPSGKLFLKSVVRSIPASDSPLRDTLDALLGGPTGQELNLGLLSMIPAGSRLLS
                     ATVKGDTAYLDFSESFRFNDMGLDGMNAQLRQIVYAATEFPTVKSVQLLIGGKRVTYL
                     GTEGARIDKPLTRDSLQPDSP"
     gene            complement(3304..4092)
                     /locus_tag="VFH83_13295"
     CDS             complement(3304..4092)
                     /locus_tag="VFH83_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET6487395.1"
                     /translation="MATRRVRLIDLLSGLHPDRDPKELYAEVLRGMVSVDGVPVTKPG
                     VLVPAHARVVVHARPAYVSRGGDKLEAAVREWGLPVEGAVWLDAGCSTGGFTDCLLAH
                     GAARVHAVDVGVGQLDWRLRTDPRVTVREGINVMALGPSDLDPPADRAVADLSFRSLR
                     GAAFHILGLAREAWGVFLVKPQFEWAEPSKDFHGVVRDRDTVRGILAGLLEGLASEGV
                     AAEKALRSPIRGQKGNREYLVLLRRVGAPAAIQVAELLRAVGLE"
     gene            complement(4102..4911)
                     /locus_tag="VFH83_13300"
     CDS             complement(4102..4911)
                     /locus_tag="VFH83_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HET6487396.1"
                     /translation="MAQHIRVLILGDVVGQPGCRALFVGLPELARRVSADLVIANGEN
                     AADGFGLTPDIATRMLASGVHVITSGNHIWQKREILPFLATSDTTLRPENYPVLSGAE
                     AIPGKGHCLVTVRDFQVLVINLEGRVNLSPLRDPMTVGRSLLKQYRNKVRASIIDFHA
                     ESVDEKEALALYLDGEVSAVVGTHTHVQTADERILPKGTAYITDIGMTGPLDSVIGMK
                     KETAIARSLSQMPLKMEVENSAAEIQGVLVTIDPATSKAVALERIRVPSLV"
     gene            complement(4920..>5375)
                     /locus_tag="VFH83_13305"
     CDS             complement(4920..>5375)
                     /locus_tag="VFH83_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6487397.1"
                     /translation="IGKGIETDGDQNHAEIGMELVRKLGEDPRVINAIGSHHGDLEPE
                     SVEAVIVQIADAISASRPGARKETLDNYLKRLENLEKIAEDFPGVDKAYAIQAGRELR
                     ILVNNDTVSDDAAKELAKSIAKRIENELKYPGRIKVTIIREMRVVEYAR"
BASE COUNT          862 a         1901 c         1745 g          867 t
ORIGIN      
        1 agccccgccc tgggggctgc gtagagcccc gccctggggg ctgcgtagag ccccgccctg
       61 ggggctgcgt agagccccgc cctgggggct gcgtagagcc ccgcccttcg cgccgcgacg
      121 atcttcgcca ccggcctcca ggagctgggc ggcaaggtcc gcgagctcac ccagcccgcg
      181 gccgtgagcg gcagagacgc cgaggacgtg cgagaagcca taggcgtggg cgttccagac
      241 catcgggtcc cgctcgggcg tgtccacctt gttcacgacg aggacgacct tgggtgagta
      301 cggccgaagc cgttccagga agtcgtcgtc cgccgccgtc gtctccagcg catccaggat
      361 catcaggatg aggtcggcct gttccgccgc ccgcagactt cggaccgaca cctgggcgtc
      421 gaccccgtcc gcgtccgccg cgaacccacc cgtgtccacc agcgtgacac gctttccgcc
      481 cagcgcgcag tccacctcca ccgggtcccg cgtgaccccg gcccgggagt gggtgatggc
      541 gcgccgccga tggaggaggg cattgaacag ggtggatttg cccacattcg gtctgcccag
      601 gatgaccacc cggggaagct ccgatgcctc cgaccgcccg ctcggggccg gcttaccagg
      661 agaagaagtc aagccgctct cccatccacc ctcgcacggc gctctcgatc tccgccccgg
      721 agccgagggg catcacccgc ctggccagcg cctccgcgtc ggacatccgc acggagcgga
      781 tgatgcgctt gatcaggggc acgccgatgg ggcccatgga gaagctgtcg agccccatgc
      841 ccaggagcac gaccgtggcc agcggatccc cggccatctc tccgcacatc cccaccggaa
      901 tgccgcgcgc gtgggcgttc ccgatcgtgg tccggatcag gcgcagcacc ccgggatgga
      961 agggctcgta caggtacgcc accttctcgt tgccgcgatc caccgccagc gtgtactgga
     1021 tcaggtcgtt ggtcccgatc gagaagaagt ccgccttctg ggcgaggatg tccgacgtca
     1081 ccgccgccga aggcacctcg atcatgatgc cgaccgcgac atcgtcgcga aacgccaccc
     1141 cctcccgcag gaggtcggcc ttgacctccg ccaggagcac ctggatgcgc tccagctccc
     1201 cggcgcccga gaccaggggg aacatgatcc gcagcgcccc gtggacggat gaccgtagaa
     1261 gcgcccggag ctgggtccgc atgatctcgg gccgcgccag gcagaaccgg atggctctcc
     1321 agcccagaag ggggttgccg tcctggtcga tctggaaccc cggcatcagc ttgtccccgc
     1381 ccaggtccag cgtgcggatg gtcacgttgc gacccgccat agaggtcagg gccagcgtat
     1441 acgcctggta ctgctcgtct tccgacggga accggttcgg ctggagaaag agaaactcgg
     1501 atcggaacaa accgacgccg tccgcgccgt gagccaggac cgcaggcgcc tcctcgggga
     1561 actcgatgtt cgcctcgatg gcgatgcgct ttccgtccag cgtctcggcc ggcagctcct
     1621 tcaggccctt caggacaacg tacttccgtt cccaatcctt gcggcgtcgg ttgtagacct
     1681 cgcgggtcgg ggcatcgggg tggacgatca ccactccccg gttgccgtcc acgataacct
     1741 cggcgtcgtt ggtgacctgg cggctgatgt ccgagagacc aaggaccgcc ggtatctcgt
     1801 gggatcgggc caggatggcg gtatgggacg tcttgccccc cacgtcggtc gcgatcccca
     1861 gcacgatccc gcgtcgcagc cccagggtat cggaaggcat gaggttgtgc gtgatgagga
     1921 tgaccggctg gctcaggtcc gagagacggg ccctcttgcg atgcagaagc tgcgagatga
     1981 tccgctgtcc ggcatcgctc aggtccacgc tccgctccct gaggtactgg ctctcggccg
     2041 cctccagctt ggcggaaagc tgctccagga tggaaaggag cgtccactcg acgttgtgat
     2101 gggcgcgctc cagctccgcg cggacctggc tcttcagctc cggatcctcg aacatgagga
     2161 gctgggcctc gaacagggcg gcgtccctac cggtggccag gccgcggacc tcctcggcag
     2221 cctggccgag ggctaccagg aaccgttcgt tctcgaactc gacctgggac gcctggatgt
     2281 cgtagcgtgg gacggtcagt ttgtcctcga ggaccagcaa tgctttcccc cgcgcgatgc
     2341 cgggggaagc gctgatcccc gtgaactcca tcatggcggg agtgtaccgg cattcaatgg
     2401 ccctgtcaaa cgatccgatc tatagaggga gagatggcag caacaaggac atcccggaag
     2461 aaggctcagg cgtcgaacag acgcaccctt gggtgcctgt tctgggtgtg cctggtcctc
     2521 gtgatcgccg cggtgggcta cgccgcgcgt gtccccctgc aggacgcctt cggacgactc
     2581 gtatccacgc tgggcggcaa gcacggcggc ccggtgactc cgccgcaggt caccgtcagc
     2641 cccatctccc aaccgcaggg gcagacggcg ccggccacca accctccccg gccgacaaag
     2701 caggctccga tcccttcggg tcaaagcccg gggacggctc ccgccaatcc tcccgcgcag
     2761 aagacgcaga gcggtccggg acagcagccg acccaaccgg cgcagggcgg gcagccggcg
     2821 cagcagcctc cgcaacaggc cccgggcgcg cagcccgccc agcaggcgcc cgtgacgcag
     2881 cagaaagctc aggcccgcaa ggcccgcctg tacttcgtgg ccgtagatcc gtccggcaag
     2941 ctctttctca agagcgtggt gcgctccatc ccggccagtg actctcccct tagggatacc
     3001 ctggatgccc tcctgggcgg tcccacggga caggagctca acctggggct gctctccatg
     3061 atccctgccg gctcgcggct cctcagcgcc acggtaaagg gagacaccgc ttacctggac
     3121 ttcagcgaga gcttccggtt caacgacatg ggcctggacg gcatgaacgc gcagctccgg
     3181 cagatcgtct acgccgccac tgagtttccg accgtgaaga gcgtgcagct attgattggc
     3241 ggaaaacggg tcacttacct gggcacggaa ggggcacgga tcgacaagcc tctcacccgg
     3301 gactcactcc agcccgacag cccgtagcag ctccgccacc tggatggcgg ccggggctcc
     3361 cacccttcgc aggagcacca ggtactcccg gttccccttc tgaccgcgga tgggagagcg
     3421 cagagccttc tcggccgcca cgccctcgga ggccagccct tccaggagcc ccgccaggat
     3481 gccacggacc gtgtcgcggt cccggaccac gccgtggaag tccttggatg gctccgccca
     3541 ctcgaactgt ggtttcacca ggaacacgcc ccaagcctca cgcgccaggc ccaggatgtg
     3601 gaatgcagcg cccctcagcg agcggaagga gaggtcggcc acggcgcggt ccgcgggagg
     3661 atcgaggtca ctcggaccga gcgccatcac gttgatgccc tcccgcaccg tcacccgcgg
     3721 gtccgtccgc agcctccagt cgagctgccc cacgcccacg tccacggcgt gcacgcgcgc
     3781 ggcgccgtgg gcaaggaggc agtcggtgaa cccgccggta gagcagccgg catccagcca
     3841 gaccgccccc tcaaccggca agccccattc ccgcaccgcc gcctccagct tgtctccgcc
     3901 gcgggagacg taagcggggc gggcgtgcac gaccacgcgc gcgtgcgcgg gcaccagcac
     3961 gcccggtttg gtcacgggca cgccgtccac ggacaccatg ccgcgcagca cctccgcgta
     4021 cagctccttg gggtcccggt ccggatgcag gccgctcaga agatcgatca gccggaccct
     4081 gcgggtcgcc atgcggcgcc gttacaccag ggaggggacg cggatgcgct ccagggccac
     4141 ggctttggag gtagcgggat cgatggtcac caggactccc tggatctcgg ccgcggagtt
     4201 ttccacttcc attttgagag gcatctggga gaggcttcgg gcgatcgccg tctctttctt
     4261 catgccgatc acgctgtcca gcggcccggt catcccgatg tccgtgatat aggcggttcc
     4321 cttgggcagg atccgttcgt cggcggtctg cacgtgggtg tgggtaccga ccaccgccga
     4381 gacctcgccg tccaggtaga gcgccagggc ctccttctca tccaccgact cggcgtggaa
     4441 gtcgatgatg gatgcccgga ccttgttgcg gtactgcttg agcagggacc gacccaccgt
     4501 catgggatcc cgcagagggg acaggttgac ccgtccctcc aggttgatca cgagcacctg
     4561 gaagtcgcgg accgtcacca ggcagtgccc cttgccgggg atcgcctccg cgccgctcag
     4621 cacggggtag ttctcgggcc gcagcgtagt gtcgctcgtc gccaggaaag ggaggatctc
     4681 gcgcttctgc cagatgtggt tgccggaggt gatcacgtgg accccggagg ccagcatccg
     4741 agtggcaatg tccggggtca acccgaatcc gtccgccgcg ttctcgccgt tggcgatcac
     4801 caggtccgcg gagaccctgc gcgccagctc cggcagcccg acgaacaggg ccctgcatcc
     4861 cggttgtccc acgacatccc ccaggatcag gaccctgata tgctgcgcca cgccggggcc
     4921 tatcgggcgt actcgacgac ccgcatctcg cggatgatcg ttaccttgat gcgccccgga
     4981 tatttcagct cgttctcgat gcgcttggcg atggacttgg ccagctcctt ggcggcgtcg
     5041 tcgctcaccg tatcgttgtt gaccaggatg cgaagctcgc gccccgcctg gatggcgtag
     5101 gccttgtcca ctcccgggaa gtcttcggcg atcttctcga ggttctccag ccgcttgagg
     5161 tagttgtcca gcgtctcctt gcgggcgccg ggccgggacg ccgagatggc gtcggcgatc
     5221 tgcacgatca ccgcctccac gctctccggt tccaggtctc cgtggtggga tccgatggcg
     5281 ttgatgaccc gggggtcctc cccgagcttg cggacgagct ccatgccgat ctcggcgtgg
     5341 ttctggtcgc cgtcggtctc gatgcccttg ccgat
//
LOCUS       DASRZS010000549         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3372447, whole genome shotgun sequence.
ACCESSION   DASRZS010000549 DASRZS010000000
VERSION     DASRZS010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3372447"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..998
                     /locus_tag="VFH83_13310"
     CDS             63..998
                     /locus_tag="VFH83_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017811527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6487398.1"
                     /translation="MSTRDDPSAFPRTAPRRAPVDRSQVAVQVIGYAVITLFSLFCLF
                     PFVLMITNSFATDASVRANGLRLVPSAFTTLAYKVVLVYNPRQIVGAYIVTSLLTAVG
                     TVFGLFMVGMVGYALQRRDFPIRNGISFFIYFTTLFSGGLIPYYLLVTRYMGLQNNYL
                     AILLPSLMSPFLVILMKSFLKGIPYEVTESAKIDGASDFRIYWSIIQPMALPAMATVG
                     LFLALNYWNEWYNAMLFIPQLKYQPLQLFLYQVINKADFIRSSSAAANIPIQDLPGET
                     LKMAVAVVATGPIILLYPFVQRYFVQGIVVGAVKG"
     gene            complement(1026..1508)
                     /locus_tag="VFH83_13315"
     CDS             complement(1026..1508)
                     /locus_tag="VFH83_13315"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6487399.1"
                     /translation="MKPRWTRSQRFERAQTLTLDGAPSDVFPLLCPVLEYEWLPRWKC
                     RMFYADSGVAERDAVFHTRETLLRSAVWTVITYEPDRLIEYLIVIGRDAIIRLSLSLE
                     AKAEKTLLGWRMRFTLTSAPGRAMVGRRFSEAGFQKMMRARTEELNHYLTTKTMISRR
                     "
     gene            complement(1542..>2055)
                     /locus_tag="VFH83_13320"
     CDS             complement(1542..>2055)
                     /locus_tag="VFH83_13320"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6487400.1"
                     /translation="SRLGELFGISRSTLLYYDAKGILAPSGRTLAGYRMYSDQDRARL
                     QTIVLFRGLGLPLRAIKEYLDNPKDGATALLLRRMFSINEQLNRLADQQKTVLALIEK
                     DGTLKGAKRRLLAMKDLGSKAGLSASNYRRVHEAFERASPETHRQFLRHLGFTDAEVR
                     RLLKGLSKGS"
BASE COUNT          346 a          686 c          625 g          398 t
ORIGIN      
        1 ctggcgaact ggggcgtccg caggatcgac cccgattacg cgttgttcta gggaggcgcc
       61 ggatgagcac ccgggacgac cccagcgcct tccctcggac ggcgccccgt agagcacccg
      121 tggaccggtc ccaggtggcc gtgcaggtca tcgggtacgc cgtgatcacg ctcttctcgc
      181 tgttctgcct ctttccgttt gtcctcatga tcacgaactc cttcgcaacg gacgcgtccg
      241 tgcgggcgaa cgggctgcgg ctcgtgccca gcgcattcac gaccctggcc tacaaggtcg
      301 tcctggtgta caaccccaga cagatcgtgg gcgcgtacat cgtcacgtcc ctgctcacgg
      361 cggtgggcac ggtgttcggc ctgttcatgg tcgggatggt ggggtacgcc ctccaacggc
      421 gggacttccc catccgcaac gggatctcct tcttcatcta cttcacgacg ctcttctcgg
      481 gaggtctgat cccgtattac ctcctggtca cccgctacat ggggctccag aacaactatc
      541 ttgccatcct gctcccctcc ctcatgagtc ccttcctggt gatcctcatg aagagcttcc
      601 tgaagggcat cccgtatgag gtgaccgagt cggcgaagat cgacggggcc agcgatttcc
      661 ggatctactg gtccatcatc cagccgatgg cgcttcccgc catggccacg gtcggtctgt
      721 tcctggccct gaactactgg aacgagtggt acaacgcgat gctgttcatc cctcagctca
      781 agtaccagcc gctccagctc tttctctacc aggtcatcaa caaggcggat ttcatcagga
      841 gctcgtcggc cgccgccaac attcccatcc aggacctgcc gggagagacc ctgaagatgg
      901 cggtcgccgt ggtagccacc ggacccatca tcctgctcta tccctttgtg cagcgctact
      961 tcgtccaggg catcgtggtg ggcgcggtga aggggtaacg gagcccccgc caggcggccg
     1021 gcccgtcatc gccgggaaat catcgtcttc gtcgtgaggt agtggttcag ctcctcggtg
     1081 cgcgcgcgca tcatcttctg aaagccggct tcggagaacc ttcgtccgac catggccctg
     1141 cccggggccg aggtgagcgt gaaccgcatc cgccagccca ggagcgtctt ctcggccttg
     1201 gcctccaggg aaaggctcag gcggatgatg gcatcgcgcc cgatcacgat gaggtactcg
     1261 atgagccggt ccggctcata ggtgatcacg gtccacacgg cgcttcgcag gagcgtctcc
     1321 cgcgtgtgga agacggcgtc tctctccgcc acgccggagt ccgcgtagaa catgcggcac
     1381 ttccatcgcg gaagccactc gtactcgagc accgggcaga gcagcgggaa cacatcgctg
     1441 ggagcgccgt ccagcgtgag ggtctgcgcc ctctcgaatc tctgactcct ggtccatcgg
     1501 ggtttcatgc caggaacctg gactgtgaag ccgtagacgg gtcaagaccc tttcgaaagc
     1561 cctttcagga gacgccgaac ctccgcatcc gtgaacccga ggtgacgcag gaactggcgg
     1621 tgcgtctcgg gggacgcgcg ttcgaacgcc tcgtggacgc gccggtagtt ggacgccgag
     1681 agacccgcct tcgatcccag gtccttcatc gccaggagac gccgcttcgc ccccttgagg
     1741 gtcccgtcct tctcgatcaa cgccaggacc gtcttctgct ggtccgccag ccggttgagc
     1801 tgctcgttga tcgagaacat ccgcctcagg agcagggcgg tcgccccatc cttcgggttg
     1861 tccagatatt ccttgatggc acggagaggc aaccccaggc ctctgaagag cacgatggtc
     1921 tggagccgcg cgcggtcctg gtccgagtac atccggtagc ccgcgagggt ccgccccgac
     1981 ggagcaagga tccctttggc gtcgtagtag agaagggtgg accgagagat gccgaacagc
     2041 tcgcccagcc ggctg
//
LOCUS       DASRZS010000550         5980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_306409, whole genome shotgun sequence.
ACCESSION   DASRZS010000550 DASRZS010000000
VERSION     DASRZS010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306409"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2295)
                     /locus_tag="VFH83_13325"
     CDS             complement(<1..2295)
                     /locus_tag="VFH83_13325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6487401.1"
                     /translation="MLKNAPRYRSSPFPGDERRALRTRITNIVGFLYAGALVVGGAVF
                     VPLFAIHKVESLSIVLGLGILFGVSQWGFRQAELALRRSENRFQMFMEHFPGLAYVKD
                     FDGRIVFANEGFRSLVGVSPEDLPGKTNEEVFGPDFGAKVTADDRRVLESGVAEDIHE
                     VWGGRSWTTYKFVVDQPGDLPLVAGVTVDTSRRQDAEDELARSRELLRAVLDGTSDLI
                     WSVDSDTFGLLNWNRAYEQYVLRSRHLAVKAGMRPADLFPEGSPLIQAWDGYYRKALS
                     EGPFSMEYVLSTGDQILLVGISPMHQQGRPIGISVFGKDITERKRMESALRESEGLFR
                     ALFQQAGDSILIIDPSAPNGALIVDANEAACRIQGAERQELVGATVGSIDDPEGQSLS
                     AGRIARAVAGEHMMFETTHVRRDGSTYPVEISAKAVQRSGRPPLIFAIERDISERRRA
                     EESLRRSERTLSQLFHNSGSIFAISTVLEGRFVDVNSRFLEATGYRRDQVVGHTAEEL
                     RLFDPAQAARLRDLLKERGSVTGFEGSYRRSSGELGYAFVSATQIEIENAPCILLEAI
                     DITERKAAVEQMELLKASIDSAPDAAYWMDPQSRFVYVNDAGCQAVGYSREELLTMSL
                     RDVNPGATPEAWQEVWRVIKEQGTFRGRGEHRRRDGTLYPVEITSTYFRLGEREYCNG
                     YAQDLTERLRAEAERAALQTQLSQAQRMEAVGRLAGGVAHDFNNMLGVILGHADSALT
                     DLAAGQTFHEEFSEIRKAAQRSADL"
     gene            2452..3300
                     /locus_tag="VFH83_13330"
     CDS             2452..3300
                     /locus_tag="VFH83_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET6487402.1"
                     /translation="MRQFQISTIALSEHCGPESLNPRHESLESRLSEADRYLSMAAKA
                     GTDFVCLPESFAHHEWAEHTMHEHAEELGKGPVSSFCADWARKGRMNVVCATTILFGG
                     KPYNTAILFDRTGKPAAWYQKVHLADGKEGEGRHLESGNRLDVFQIEGLTIGFQICYD
                     LNFPEGCRTLALKGAELIFWPTMWDLDLCGFTEHIVKARAMENLLTIVASAYADYPPG
                     KPPALSAQRTVKMTAVVDWAGFTLASAGTTPGLASAIVDFDRVPFHQMTREYMKKLRR
                     PEVYEL"
     gene            3297..4334
                     /locus_tag="VFH83_13335"
     CDS             3297..4334
                     /locus_tag="VFH83_13335"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6487403.1"
                     /translation="MTPRERVLAAIEHQSPDRIPCDLWAEPGVWERLRRRLGVRTEDE
                     VRAALQVDVRYLSPRHPPDTVINGVRQNAWGERWEKTSTVYGVEWEHTRGALYDAQSV
                     ADLEAFPWPTCDQVDYSAIAEEARRYEGHAIFCGNADFFERPGLVRGLENILVDVIEN
                     PEMVEYLEERFLTFFIEDFHRTMEAANGKVDAFLALTDLGTQDRLLMGRDAMERFIFA
                     PLRRLAHVVHAEGVRLLFHSCGAVREVIPDLIACGVDILNPLQPAAKGMEPSGLKRDF
                     GRDVAFHGGIDIQYVLPVKTPQEVRAEVRRVAGILGQQGGYVLSASHNIQQDTPTENI
                     LAMYDPEARRL"
     gene            4624..5913
                     /locus_tag="VFH83_13340"
     CDS             4624..5913
                     /locus_tag="VFH83_13340"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6487404.1"
                     /translation="MRRIRVSLWLLGLLLLLAPLAWAGGTKEATTSPAGKAAIQGSIR
                     VWGDTQNETITSKPFQEINAAFMKEYPNVKVTYDFGENNETLQVAIQSDSLPDAFYLG
                     GNKNPQTAELARAGKLLDLTGKIDNMSMYTDQNIIDYITVDGKVICSPTGFLDSQLVY
                     YNKDIFQKQGISLPKTFNDFLALCDKLLAAGIQPIAMPGAAVWDNSWAAFAFFADFAS
                     DALLRIYHGKGTWNDPDIEKAFNYTRLLAEKGYYGKNYAGMNQQDAQLSFTSGKAAMY
                     VDGTWEESVYGGSGLNLGRFYIPNDQGVRIGCLSVSNYMTYAVSAKTKYPEAAVAYVN
                     FLAQLPAQQIMENDAFLPIMIKGIKPANDVVNELSGFDRLGAQMHQVATACDTDKSKP
                     DDLLMSTIIPQLIQLKITGKEACAMMDKASTYPKPTN"
BASE COUNT         1127 a         1929 c         1926 g          998 t
ORIGIN      
        1 caggtccgcg gagcgctgtg ccgccttgcg gatctccgag aactcctcgt ggaacgtctg
       61 cccggcggca aggtcggtca gcgccgagtc ggcgtgcccc aggatgacgc ccagcatgtt
      121 gttgaagtca tgggccacgc cgccggccag cctgccgacc gcctccatcc gctgcgcctg
      181 ggagagctgc gtctgcagcg cggcgcgctc cgcctcggcc cgcagccgct ccgtcaggtc
      241 ctgggcataa ccgttgcagt actcccgctc gcccagacgg aagtacgtgg aggtgatctc
      301 cacggggtac agggtcccat cccgtcggcg atgctcgccc ctgccccgga aggtgccttg
      361 ctccttgatg acgcgccaga cctcctgcca cgcctccggc gtggcccccg ggttgacgtc
      421 ccgcaggctc atggtcagga gctcttcgcg gctgtacccc acggcctgac agccggcgtc
      481 gttcacatag acgaacctgc tctgagggtc catccagtag gccgcgtccg gagcgctgtc
      541 gatggaagcc ttcagaagct ccatctgctc caccgcggcc ttgcgctcgg tgatgtcgat
      601 ggcctccagc aggatgcacg gagcgttctc gatctcgatc tgcgtcgcgg agacgaatgc
      661 atacccgagc tctcccgacg agcgccggta gctcccctcg aatccggtga ccgagccgcg
      721 ctccttgagg agatcccgca accgggcggc ctgggccgga tcgaagagac gcagctcctc
      781 ggcggtatgc cccaccacct ggtcccgacg gtacccggta gcttccagga aacggctgtt
      841 gacgtccacg aatcggcctt cgagcacggt ggagatggcg aagatgctgc cgctgttgtg
      901 gaagagctgg gatagcgtcc tctccgagcg tcgaagggat tcctccgcgc gcctgcgctc
      961 gctgatgtcg cgctcgatgg cgaaaatgag cggaggccgg ccggaccgtt ggaccgcctt
     1021 ggcgctgatc tccaccgggt acgtgctccc gtcccgccgg acgtgggtcg tctcgaacat
     1081 catgtgctcc ccggccacgg ctcgggcgat ccgcccggcg gagagtgact ggccctccgg
     1141 gtcatcgatg ctgcccacgg tcgcgcccac cagctcctgg cgctccgccc cctggatccg
     1201 gcaggcagcc tcgttggcat ccacaatcag ggcgccgttc ggagcgctcg gatcgatgat
     1261 gaggatggag tcgcccgcct gctggaagag ggcgcggaag agcccctcgc tctcccgcag
     1321 cgccgactcc atgcgcttgc gctccgtaat gtcctttccg aagaccgaga tgccgatggg
     1381 acggccttgc tggtgcatcg ggctgattcc caccaggagg atctggtctc ccgtggaaag
     1441 gacgtactcc atggagaacg gaccctccga aagggccttg cggtagtacc cgtcccacgc
     1501 ctggatgagc ggagagccct cggggaagag gtccgccgga cgcattccgg ccttcacggc
     1561 caggtgccga ctccgcagga cgtactgctc ataggcgcga ttccagttga gaagcccgaa
     1621 ggtgtccgag tccacggacc agatgaggtc cgaggtgcca tccaggacgg cccgcagcag
     1681 ctcccgggac cgtgccagct cgtcctcggc gtcctgacgc cggctcgtat cgacggtaac
     1741 gccggctacc aggggcaggt ccccgggctg atccaccacg aacttgtacg tggtccacga
     1801 tcgaccaccc cagacctcgt ggatgtcttc ggcgacaccg gactccagca cccgtcggtc
     1861 atccgcggtg acctttgcgc cgaagtccgg gccaaacacc tcctcgttcg tctttcccgg
     1921 cagatcctcg gggcttactc ccaccaggga ccggaatccc tcgttggcga agacgatcct
     1981 cccgtcaaag tccttcacat acgccagacc ggggaagtgc tccatgaaca tctggaagcg
     2041 gttctcgctc ctgcggagcg ccaactcggc ctgccggaaa ccccactggg agacgccgaa
     2101 gaggatcccc agacccagga cgatggacag ggactctacc ttgtggatcg cgaacagagg
     2161 cacgaagacc gcgcctccga caaccagggc ccccgcgtag agaaagccca cgatgttggt
     2221 gattcgggtc cgcagagctc tccgttcgtc gcccggaaac ggagaagaac gataccgcgg
     2281 agcattcttc agcatcctgc tctcctgcag acggcagcat acctgaggtg ggagtttgcg
     2341 acgggtggac gcaacctacc atcccgaggc cgggctgtca accgggacga ggggcccggc
     2401 cgggaccgcc gggttgactc cactgactca gggtcgtaga gtcgacagcc gatgaggcag
     2461 ttccagatca gcacgattgc tctgagcgag cactgcgggc cggaaagcct gaaccccagg
     2521 cacgagagcc tggagagcag gctgtcggag gccgaccgct atctgtccat ggccgccaaa
     2581 gccggaacgg acttcgtctg cttgccggaa agcttcgcgc accacgagtg ggcggagcac
     2641 accatgcacg agcacgcgga ggagctgggc aagggccccg tctcctcgtt ctgcgccgac
     2701 tgggcccgca agggccggat gaacgtggtg tgcgccacga cgatcctctt cgggggaaag
     2761 ccctacaaca cggcgatcct gttcgaccgg acggggaagc cggccgcctg gtatcagaag
     2821 gtccatctgg ccgacggcaa ggagggcgag ggacgccacc tggagtccgg caatcgcctg
     2881 gacgttttcc agatcgaggg cctgaccatc ggctttcaga tctgctatga cctgaacttc
     2941 cccgaggggt gccggacgct ggccctcaag ggcgcggagc tgatcttctg gcccaccatg
     3001 tgggacctgg acctctgcgg cttcaccgag cacatcgtga aggcgcgcgc catggagaac
     3061 ctcctcacca tcgtggcctc ggcgtacgcc gactaccctc ccggcaagcc gccggcgctg
     3121 agcgcccagc ggacggtgaa gatgacggct gtcgtggact gggccggctt caccctggcc
     3181 tctgcgggga ccacgccggg gctggccagc gccatcgtcg acttcgaccg ggtgccgttc
     3241 caccagatga ctcgggaata catgaagaaa ctgcgccgcc cggaggtcta cgagctgtga
     3301 cccctcggga gcgcgtcctc gccgccatcg agcatcagag cccggaccgg atcccatgcg
     3361 acctgtgggc cgagcccggt gtctgggagc gcctgaggcg gcgcctgggg gtcaggacgg
     3421 aggatgaggt ccgcgccgct ctccaggtgg acgtccgcta tctctcgccg aggcacccgc
     3481 cggacaccgt catcaacgga gttcgccaga acgcatgggg ggagcgctgg gagaagacct
     3541 ccaccgtcta cggcgtggaa tgggagcaca cccgcggcgc gctctacgac gcccaatccg
     3601 tagccgacct ggaggctttc ccgtggccca cctgtgacca ggtggactac tcggccatag
     3661 cggaagaagc gcgccgatac gaagggcacg cgatcttctg cggcaacgcc gacttcttcg
     3721 agaggcctgg gctggtgagg ggcctggaga acatcctggt ggacgtgatc gagaaccccg
     3781 agatggtgga gtacctggag gagcggttcc tcaccttctt catcgaggat ttccacagga
     3841 ccatggaagc ggccaacggg aaggtggacg cgttcctggc gctcaccgac ctgggcacgc
     3901 aggaccggct cttgatgggt cgggatgcaa tggagcggtt catcttcgct cccctgcggc
     3961 gtcttgccca tgtggtgcac gcggaggggg tcaggctcct gttccactct tgcggcgcgg
     4021 tccgagaggt gatcccggac ctgatcgcct gcggcgtgga catcctgaat cccctgcagc
     4081 ccgccgccaa ggggatggag ccttcagggc tgaagcggga cttcggccgg gacgtggcgt
     4141 tccacggcgg catcgacatc cagtacgtgc ttcctgtcaa gacgccgcag gaggtgcgcg
     4201 cggaggtccg ccgcgtcgcg ggcatcctcg gccagcaagg cggctacgtg ctctcggcat
     4261 cgcacaacat ccagcaggac acccctaccg aaaacatcct ggcgatgtac gaccccgagg
     4321 ctcgtcgcct ctagcggggt tgggacgcgg cggggttggt tcggcggggg tcggggcggg
     4381 gatcggggcg gcgggggtcg gggctgcctc tcgaatacgg ttcggttgac aggctcgagg
     4441 gccgcgacct acaatcccgc gcttgcatgc agtatccaag aggaggacaa tgagacgcag
     4501 gtagatcggc aagcgcaagc cgcgctatgt cgaggtggct ccccttgaat ccacgttgta
     4561 caccaacggc ggggacgggc gggagcggtt gaggatgatc actggatcct aaggaggaat
     4621 gcgatgagac gaatcagagt ttccctttgg ctgctcggtc tgcttctgct gctggcgccc
     4681 ttggcgtggg caggcgggac aaaggaagca acgaccagcc cggccgggaa ggccgccata
     4741 cagggcagca taagagtctg gggcgatacg cagaatgaaa cgatcacatc aaagccattt
     4801 caggagatca atgctgcgtt catgaaggag tatccgaacg tcaaagtgac ctacgacttt
     4861 ggcgagaaca acgagacgct gcaggtcgcc attcaatccg acagcctgcc ggacgcgttc
     4921 taccttggcg gcaacaagaa cccccagaca gcggaactgg cacgtgccgg caagctgctg
     4981 gacctgaccg gcaagatcga caacatgagc atgtacacgg atcagaacat catcgactac
     5041 atcacggtcg atggcaaagt gatctgctcc ccaaccggct tcctggacag ccagctcgtc
     5101 tactacaaca aggacatctt ccagaagcag gggatctccc tacccaagac cttcaatgac
     5161 ttcctggcgc tgtgcgacaa gctgctggct gccgggatac agcctatcgc catgcccggg
     5221 gccgcggtgt gggataatag ctgggccgcg tttgctttct tcgcggattt tgcatccgac
     5281 gcgctcctga ggatctatca cggcaagggc acgtggaatg accctgacat cgaaaaggcc
     5341 ttcaactaca ccaggctgct ggctgaaaag ggctactacg gcaagaacta cgcgggaatg
     5401 aaccagcagg acgcccagtt gtcgtttacc agcggaaagg ccgcgatgta tgtcgacggc
     5461 acctgggaag agtccgtcta tggcgggtcc ggcctgaatc tgggacgctt ctatattccg
     5521 aacgatcagg gcgtgagaat cggctgcctg agcgtaagca actacatgac gtacgcggta
     5581 tcggcgaaga cgaagtaccc cgaggcggcg gtcgcctatg tcaacttcct ggcacagctc
     5641 ccggcccagc agatcatgga gaacgatgcg ttcctgccca tcatgatcaa gggcatcaag
     5701 cccgcgaatg acgtcgtgaa tgagctgtcc ggtttcgaca ggcttggcgc ccagatgcac
     5761 caggtcgcta ccgcctgtga tacggacaag tccaagccgg acgaccttct catgagcacg
     5821 atcatccctc aactgattca gttgaagatc accggcaagg aagcctgcgc gatgatggac
     5881 aaggcctcga cgtatcccaa gccgaccaac taaggaagaa gccatcgacc ctgcgcctgt
     5941 cacggactac ggcctgtcga taggcgcagg gatccgggct
//
LOCUS       DASRZS010000551         3422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3004276, whole genome shotgun sequence.
ACCESSION   DASRZS010000551 DASRZS010000000
VERSION     DASRZS010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3004276"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1168)
                     /gene="nuoH"
                     /locus_tag="VFH83_13345"
     CDS             complement(113..1168)
                     /gene="nuoH"
                     /locus_tag="VFH83_13345"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET6487405.1"
                     /translation="MGRFLYDLFYNTDTLLSGWRVSAEQAWAARGSPVVLLDLLFNLL
                     AAGAVAVFLVLNSLWIIYFERKVAARMQLRRGPNRVGPLGLFQTLQDTVKLLIKEDII
                     PDAADKGVFRVAPLIVLAATFMLFVVLPFGKGLTAGDLNVGLLYFLAVSGVSVIGILA
                     GGWASNNKYSLMGGMRSAAQIVSYEVPGVLTVLSVAMLAGSLKMGDIVKAQPVYGYVL
                     LQPIGFILFLTASNAEINRAPFDLPESDSELVSGFVTEYSAMRFAFFFLAEYTNLFLA
                     GAFATTLFLGGWRGPFLPPLVWFLLKTYFVVFLLIWVRWTFPRLRVDHLMQFSWKILL
                     PLALLNLVVTAVLVKVL"
     gene            complement(1178..2254)
                     /locus_tag="VFH83_13350"
     CDS             complement(1178..2254)
                     /locus_tag="VFH83_13350"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HET6487406.1"
                     /translation="MLNIGPQHPATHGVLRVVVTMDGEVVKEVVPHIGYVHRGLEKLF
                     ENRLYEQIIPFTDRTDYLSAIANNFAYVMAVERLLGLEVPPKVEYTRVMAAELNRIAS
                     HLLWLAAYGLDMGAFTPFLYAFRDREEIGSMIEEASGGRLTTNYSRIGGFAYELPKDI
                     FGKLGKFLSHFERKIDEYDTLLIENAIFVARTRGVGVISRERAISYGMSGPCLRASNA
                     ALDVRRDIPYSIYPKLSFDIPTGRNGDTWDRSKVRIEEMRQSVRILRQVLDGFPQGAV
                     KVRAPRSIEPPPGETYAAVEGPRGEVGFYVVSDGSRKPYRIKMRKPSFSNFSAMPEIL
                     RGLKIADLVAVMGSLDLVVPEIDR"
     gene            complement(2356..2820)
                     /locus_tag="VFH83_13355"
     CDS             complement(2356..2820)
                     /locus_tag="VFH83_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET6487407.1"
                     /translation="MTETPVLERLRARYPDAAARPGDPVLHVVIPAESVRQNLQFLRD
                     ALQFDQLTFLTAIDRPAESRLEVVYRLFSYQARAAVMVRVMLPRDGARLDTVSDLYRT
                     AEWHERETAEMFGILFVDHPDPRKLLLPDDLEGHPLRKDFTHPNLIRLPEVP"
     gene            complement(2817..3332)
                     /gene="nuoB"
                     /locus_tag="VFH83_13360"
     CDS             complement(2817..3332)
                     /gene="nuoB"
                     /locus_tag="VFH83_13360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HET6487408.1"
                     /translation="MNGLNERDLVLRMPGGQVMVSSLERMINASRSNSLWTLTFGLAC
                     CAIEMMATGASTYDWARFGFEVPSASPRRADMMIVAGTVVRKMAPIIKTLYEQMPDPK
                     YVIAMGGCAIAGGPYVYDSYTIVRGVDEIVPVDVYIPGCPPRPEALLQGLMTLQTKIR
                     AQKLQDLGGAP"
     gene            complement(3332..>3422)
                     /gene="ndhC"
                     /locus_tag="VFH83_13365"
     CDS             complement(3332..>3422)
                     /gene="ndhC"
                     /locus_tag="VFH83_13365"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET6487409.1"
                     /translation="LPLGEVLVFIAILVLALAYAWRKGALQWD"
BASE COUNT          668 a         1088 c         1131 g          535 t
ORIGIN      
        1 tcctcagggt actggaccgt gatcggcttt cggaaaagat aggagaacgt gatggcgaga
       61 cccttcgcca gggaaacgac cccgcgggcc atgctcgcca gatagctttc catcacaaca
      121 ccttcaccag aacagcggtg accaccaggt tgaggagggc caggggaagc aggatcttcc
      181 acgagaactg catgaggtgg tccacgcgga gccgcgggaa cgtccaccgc acccagatca
      241 ggaggaacac cacgaaatag gtcttgagca ggaaccagac cagcggcgga aggaacggcc
      301 cccgccaacc gcccaggaag agcgtggtgg cgaacgcccc ggccaggaac aggttggtgt
      361 actccgccag gaagaaaaac gcgaagcgca tggcgctgta ctccgtgacg aagcccgaga
      421 ccagctccga gtcggactcc ggcaggtcga acggcgcgcg gttgatctcc gcgttggagg
      481 cggtcagaaa gaggatgaac ccgatgggct gcaagagcac gtagccatag accggctggg
      541 ccttgacgat gtctcccatc ttgagggacc cggccagcat ggcaaccgag agcaccgtga
      601 ggaccccggg gacctcgtag ctcacgatct gcgccgcgga ccgcatgcct cccatcaagg
      661 agtacttgtt gttggaagcc cagccgcccg ccaggatccc gatcacggag actccgctca
      721 ccgccaggaa gtacagcaga cccacgttca ggtccccggc ggtcagccct ttgccgaagg
      781 gcagcaccac gaaaagcatg aacgtagcgg ccaggacgat gagcggcgcg acgcggaata
      841 cgcccttgtc cgccgcgtcc gggatgatgt cctccttgat gaggagcttc accgtgtcct
      901 ggagggtctg gaagagcccc aggggaccca cgcggttggg acctcggcgg agctgcatcc
      961 gcgcggcaac cttgcgctcg aagtagatga tccacaggct gttgagcacc aggaacaccg
     1021 ccaccgcgcc ggcggcaagc aggttgaaga gcaggtcgag caggacgacc gggctgccgc
     1081 gcgcggccca cgcctgctcc gcggacacgc gccagccgga gaggagcgtg tccgtattgt
     1141 agaaaaggtc gtacaggaac cgtcccaccg gtccttccta tcgatcgatc tctgggacca
     1201 cgaggtccag gctgcccatc accgcgacaa ggtcggcgat cttcagaccc cgaaggattt
     1261 cgggcatcgc gctgaagttg gagaagctgg gcttgcgcat cttgatccgg tacggtttcc
     1321 gacttccgtc gctcaccacg tagaaaccaa cctccccgcg aggcccctcc accgccgcgt
     1381 aggtctctcc cggcggcggc tcgatgctcc gaggcgccct caccttcacc gcgccctgcg
     1441 ggaacccatc caggacctgg cgcaggatcc gcaccgattg ccgcatctcc tcgatcctca
     1501 ccttcgaccg gtcccaggta tcgccgtttc tcccggtcgg gatatcgaag gacagcttcg
     1561 ggtagataga atacggaatg tcccgccgaa cgtccaacgc ggcgttcgac gcccgcagac
     1621 aggggccgct catgccgtaa gagatggccc gttcccggct gatgacccca acgccgcgcg
     1681 tccgggccac gaagatggcg ttctcgatca ggagggtatc gtactcgtcg atcttgcgct
     1741 cgaaatggga gaggaacttc ccaagcttgc cgaagatgtc cttcggcagc tcgtaggcga
     1801 accctccgat gcgggagtag ttggtggtga gtcgtccgcc ggaggcctcc tcgatcatgg
     1861 acccgatctc ctcccgatcg cggaaggcgt agaggaacgg agtgaaggcg cccatgtcca
     1921 gcccgtaggc ggcaagccag aggagatggc tggcaatccg gttcagctcc gcggccatca
     1981 cccgcgtgta ctcgaccttg ggcggcacct ccagacccag gagcctctcc acggccatga
     2041 cgtaggcgaa gttgttggcg atggcggaaa gatagtcggt gcggtcggtg aacgggatga
     2101 tctgctcgta gaggcggttc tcgaagagct tctccagacc ccgatggacg tacccgatgt
     2161 gggggaccac ctccttgacc acctcaccgt ccatggtcac caccacccga aggacaccgt
     2221 gcgtggccgg gtgctggggg ccaatgttca ggatgaactc gtcggatgtg gtcggcgccg
     2281 cggatgggga cgggccggcg gatgcggggg gctgcgcggc ggatggaatc cgggttcctt
     2341 ccacggtgct tcctcctacg gcacttccgg cagacgaatg aggttggggt gagtgaaatc
     2401 cttcctcaac ggatggccct ccaggtcatc cggtagaagc agcttgcggg gatccggatg
     2461 gtcgacgaac aggatcccga acatctccgc ggtctcccgc tcgtgccatt cggcggtccg
     2521 gtagagatcg ctcaccgtat ccagccgggc tccgtcccgc gggagcatta cgcggaccat
     2581 gacggcggcc ctggcctggt aggagaagag ccggtagacc acctccagcc gggactcagc
     2641 cggacggtcg atggcggtga ggaaggtgag ctgatcgaac tgcagcgcgt cgcgaaggaa
     2701 ctggaggttc tggcgcacgg actcggcggg gatcaccacg tggaggaccg ggtcccccgg
     2761 ccgcgcggcg gcgtccgggt accgggcgcg cagacgctcc aggaccggtg tctccgtcac
     2821 ggggctcctc ccaggtcctg gagcttctga gcgcggatct tcgtctggag ggtcatcaga
     2881 ccctggagca gcgcctcggg acgcggcgga cagccgggga tgtagacatc gaccggcacg
     2941 atctcatcga ccccacggac gatcgtatag gagtcgtaga catagggacc gccggcgatg
     3001 gcgcagccgc ccatggcgat cacgtacttg ggatccggca tctgctcgta gagcgtcttg
     3061 atgatcggcg ccatcttgcg caccacggtc cccgccacga tcatcatgtc agcccttcgc
     3121 ggcgacgccg aggggacctc gaagccaaag cgcgcccagt cgtacgtgga ggcgccggtg
     3181 gccatcatct cgattgcgca gcaggccagg ccgaacgtga gggtccagag ggagttggag
     3241 cgggaggcgt tgatcatgcg ctccagggag gagaccatga cctggccgcc cggcatccgc
     3301 agaacgaggt cgcgctcgtt cagaccgttc atcagtccca ctggagggcc cctttgcgcc
     3361 acgcatacgc aagcgccagg accaggatgg cgatgaagac gaggacctct ccgaggggaa
     3421 gg
//
LOCUS       DASRZS010000552         9083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_307135, whole genome shotgun sequence.
ACCESSION   DASRZS010000552 DASRZS010000000
VERSION     DASRZS010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9083
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307135"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VFH83_13370"
     CDS             complement(<1..455)
                     /locus_tag="VFH83_13370"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET6487410.1"
                     /translation="MPSLYVTDLDGTLIRDDLTLSPFSRARLTELLNDGAAITIATAR
                     SIVSLTAILGDLPFRLPIVEFNGSYLSDYHTRRHLVVNAIEPDVATETYRRVQAAGMR
                     PFISAFDGANDLLFYREISNPGENAYLRERQSFLDRRLRQVPDLEPCLS"
     gene            complement(459..1706)
                     /locus_tag="VFH83_13375"
     CDS             complement(459..1706)
                     /locus_tag="VFH83_13375"
                     /inference="COORDINATES: protein motif:HMM:NF013473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6487411.1"
                     /translation="MPSRRTMPAAWASYFLLFLSTAIFVPYLQLYLKARGFSPSQIGI
                     LLACLELAGLLGPILLGRLADRHARYRGLFALSLVVTVAAFAPLQLTSRFLWFALGLG
                     VMGFAYRSTTPLLDSLVGKTLTDPTRQYGLLRVAGSFAFIAVSVVLQFTSLSTGKSSV
                     GILVAFSAAALVALAAVPFLVPAGRDGSAQARVAPNGAAAVDSAVLSMRQSGFTPAFW
                     IVMGIIFLGRFGIGSYYSFFSLYLQQTFPGVGISLLWAIGPVAEVVAMYFSGRLIARR
                     GIRFMLVLSLASISVRLSLFVIAPSIGVVAGAQLLHAFTFGTFHTTAIAYINSTIARE
                     RRGLGMALYTTCGVGLPSLLASLVGGFVLQAHGFAVLFLSYAVAPVIGIALLTVLGAR
                     LLPRPTAAQGRGPGAVTRSAVNG"
     gene            complement(1742..2713)
                     /locus_tag="VFH83_13380"
     CDS             complement(1742..2713)
                     /locus_tag="VFH83_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6487412.1"
                     /translation="MNKRRIGKTDVEVTPIGLGCWQFSQSSSWFSNFWETLNQRTIDE
                     VVATALKGGIDWFDTAELYGRGTSEKGLAAALKTAGVKPGSVRIATKWWPVLRSARTI
                     PANIETRIACLAPYPVDLYQVHQPWSVSPVPGQMREMAKLLLDHRIRAVGVSNFSARS
                     MELAHATLASQGLVLASNQMRFNLLDRHIERNGVLAAAKRLGITIIAYSPLAQGVLTG
                     RFHEDPSQVAKVSRMRRMMGTMSPLRLARTAPLLDELRSIARAHGVSIAQVALSWTVS
                     FHGDTVVAIPGASRMAQAEQSAGALSVRLSPQELNRIDELSRKVSSR"
     gene            complement(2727..3704)
                     /locus_tag="VFH83_13385"
     CDS             complement(2727..3704)
                     /locus_tag="VFH83_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487413.1"
                     /translation="MAALISVKDLVKNFPLRGTIFDVLSPRRMAVHAVDRVSFDIGEG
                     EVLGLAGESGSGKSTTGRLLLRLTEPTSGSIQYRGRDITSFSPREMEHLRAKAQIVFQ
                     DPNSSLSPRMSVGQAISHPLEIHGIGTSSERKERVIDLMEKVGLAPARFLYRKYPHQL
                     SGGQSQRVVLARALVTGPEFLVADEPIAMADVSVRALILQLMRQLKLEMGLTYLFITH
                     DLATAKYLCDRIAVMYLGRIVEVASRDDLFSRPHHPYTKALLAAVPIPDPTARHDKHL
                     PKGEIPSAINPPSGCHFHPRCPYAIPRCRQERPPLRTISPNHLSGCILD"
     gene            complement(3704..4723)
                     /locus_tag="VFH83_13390"
     CDS             complement(3704..4723)
                     /locus_tag="VFH83_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487414.1"
                     /translation="MSPTHQAQTTETAASKGNPTILEVDHLSIAYPISIGVVRAVEEV
                     SITLADGEALGLVGESGCGKSTLGLSILKLLRPPGRVTAGAIRYRGNDILGMSGQALL
                     ALRGQNIAMIFQNPLTSLNPLFTVERHFLETIHFHDPGMSKAKARAGVEKILETLGIE
                     RKRLAEYPHQLSGGMRQRIMIGLALIMNPDILIADEPTTSLDVIVEAGFTDLLTTLRD
                     LYKLSIILITHNLGLVAEIADRIAVMYGGKIMEVATAREIFAHPLHPYTQGLIACVPN
                     ILLDQKDLTTMPGMPPDLVQPPPGCRFAARCARVMPICRQKEPPLRSHAPAHHAACWL
                     YGTRD"
     gene            complement(4720..5640)
                     /locus_tag="VFH83_13395"
     CDS             complement(4720..5640)
                     /locus_tag="VFH83_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487415.1"
                     /translation="MSTQPARQPVRTARLSPAAGLASVFRWFSSSRRKYGGEWWIMIA
                     GVLIIAVIVTMTVFPHLFTHYSPLDQNAGGQMTPPSREHLMGTDNLSRDVWARVVYGA
                     QTILGVAIAAALVSALVGIPLGLVSGFAGGLFDRILSLIMDSVYSFPGLILAIAFAAM
                     LGPGVINITLAVAVVYIPTYFRLVRGQTLSIKEEVYVEAARAIGARRGTILGRYVFPN
                     VIATVVVVLSLNIADAIMTEAALSYIGLGLPAGIPDWGMDLSMGKKFLPSGIWWMITF
                     PGLMIMLVALGFTMLGEGLAEILNPRLLER"
     gene            complement(5651..6667)
                     /locus_tag="VFH83_13400"
     CDS             complement(5651..6667)
                     /locus_tag="VFH83_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487416.1"
                     /translation="MRHLLNYLITRLLLSIPMIFILVTLVFVVLRIMPGDPVNAMLGG
                     HAPQKVIDEKKEALGLNRPITVQYVDYLNQLIHFDLGTSMIFQQKVSQPIIEKLPATI
                     ELTLFGLLASLAIGIPLGASAARRRRSGRDFGIRLYANVAYCIPVFWMGLLLQLVFGV
                     FLGWFPVSGRTGPRIDVSEFSRSGFYVLDTLRAGQVANFGDVLWHLVLPSFTLGIVLS
                     GIFVRLTRANMLDVLKSDYILAARARGIPERRVVYNHGLRNALIPIVTMLGLQFSLLL
                     AGAILTESTFSWPGMGRLLLERIYLRDYPVIQGVIIVYTLMVSVISLLVDLIYAVIDP
                     RVKY"
     gene            complement(6725..8272)
                     /locus_tag="VFH83_13405"
     CDS             complement(6725..8272)
                     /locus_tag="VFH83_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6487417.1"
                     /translation="MKRLFTILLVAALAMSASALFAQTLVYGTTDKVTDMDPASAYDF
                     HTWEIFQNVSCGLLQYTPGTTDLVPGLATGYTTNPAGDQYTFTLRKGVKFSDGTPFTA
                     QVVKWSIDRVAKLNGDPAALVTQYVKSVDVVNDYTVRFNLTGPLSYLPQLVATPTYFP
                     MDPKVYPEDKIVKDVSELNGGEIAALGPYQLTSFKRDEQAVFQANPNYFGKEPAVKKI
                     IIRYFADATTMRLALERGEIDLAFKSLNPSDIDDLLKNPKFVGTKIPGPQIRYLCFET
                     SESVFKDKRLRQAVAALINRPEINQKVYLGHNAPLYSMIPVGMSYHTDAFKTVLGDAN
                     VALAEKDLKAAGYSADNPFTFDLWYTPSHYGDTEVNMAEVLQAELQKTPLMKVTIKSA
                     EWATYKQQFNNKQMPVFFLGWYPDYIDPDDYTSPFAQTEGSKGNGIFFSSKAWDDLFI
                     QEQQTTKDATRNSVFAKLQQMWTDEVPTAPIFQGNLYVFTTKNVTGVKIGPTLIFNYN
                     TLSFVTK"
     gene            8324..8938
                     /locus_tag="VFH83_13410"
     CDS             8324..8938
                     /locus_tag="VFH83_13410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6487418.1"
                     /translation="MSIPGIERASAADAADCADLILASAEHFLPAVFGPRIRGALIRM
                     AAGRGTLFSHEHAWMARADGAAAGILLGYTGLQKAAEDPATGLRLFGALGPGLLLRLS
                     RLLRLQRSIGALAPDEYYVSNVAVRPEFRGQGIGSLLLERARAEAAEAGAAWIVLDVE
                     TDNEGARRLYERLGFSRRSETRPLVLDGTPFSFYRMGRPVRDPD"
BASE COUNT         1630 a         2750 c         3192 g         1511 t
ORIGIN      
        1 tgggagaggc acggctcgag atccggaacc tggcgcagcc gtcggtccaa gaaggactgc
       61 ctttccctga gataggcgtt ctcgccgggg ttggagatct cgcggtagaa gagcaggtcg
      121 ttggcgccgt cgaaggccga gatgaacgga cgcatgccgg cggcctgcac tctgcggtac
      181 gtctccgtcg ccacgtcggg ctcgatggcg tttacgacga ggtgccgccg ggtatggtag
      241 tcggagagat aggacccgtt gaactccacg atgggaagtc ggaacggcag atcgcccagg
      301 atcgccgtca aggacacgat gctccgcgcc gtggcaatcg tgatggcggc gccgtcgttc
      361 aggagctcgg tgagccgggc gcgtgagaac ggagacaggg tgaggtcgtc ccggatcagg
      421 gtcccatcca ggtccgtcac atacaggctc ggcatggcct atccgttgac cgcggagcgc
      481 gtcacggccc ctgggccgcg accctgagcg gccgtcggcc gcgggagcag ccgcgcaccc
      541 aggacggtca ggagcgcgat ccctatcacc ggcgcgacgg cgtaggacag gaagaggacg
      601 gcgaacccgt gcgcctgcag aacgaacccg cccaccaggc tggccaggag agatggcagc
      661 ccgacgccgc aggtggtgta aagcgccatc cccagaccgc gccgctcgcg ggcgatcgtg
      721 gagttgatgt acgcgatggc cgtggtgtgg aacgtcccga acgtgaaggc gtgcaggagc
      781 tgcgcgcccg cgacgacccc gatggacggc gcgatcacga agagggacag ccgcacgctg
      841 atggacgcga gggagagcac cagcatgaac cggatccccc gacgcgctat gagcctgcca
      901 gagaagtaca tcgccacgac ctcggccacg gggccgatgg cccacaggag gctgatccca
      961 acgcccggga acgtctgctg caggtagagc gagaagaagg agtagtagga cccgatcccg
     1021 aaccggccca ggaagatgat ccccatgacg atccagaacg cgggagtgaa gccgctctgg
     1081 cgcatcgaca ggaccgcgga gtcgacggca gccgcgccgt tcggcgcgac ccgagcctgc
     1141 gcggacccat cccgccccgc cggaaccagg aacggaacgg ccgcgagcgc gaccagggcc
     1201 gcggcgctga aggctaccag gattcccacg gaggatttcc ccgtggaaag gctcgtgaac
     1261 tggagtacca cggaaaccgc gatgaacgcg aagcttccgg cgacgcggag aagcccgtac
     1321 tggcgagtgg gatcggtgag cgtcttgccc acgagggaat cgaggagcgg ggtggtggag
     1381 cggtaggcga agcccatcac acccagcccg agggcgaacc agaggaaccg ggacgtgagt
     1441 tgcaggggcg caaacgcggc caccgtcacc acgaggctca gggcaaagag cccccggtac
     1501 cgggcgtgtc ggtccgcgag ccgacccagg aggatggggc ccaggaggcc ggccagctcc
     1561 aggcaggcaa gaaggattcc gatctgggac ggagagaacc cgcgcgcctt caggtagagc
     1621 tgcaggtacg gaacgaagat ggccgtggat aggaagagca ggaaatagct cgcccacgcc
     1681 gcgggcatgg ttcttctgga gggcatggag ggcttactgt agcagaaccg aaggggcgct
     1741 tctaccggct cgacactttt cgggaaagct cgtcgatccg attcagctcc tgcggcgaga
     1801 ggcggaccga aagcgcgccg gcgctctgct ccgcctgggc cattcgcgag gcgcccggga
     1861 tggccactac ggtgtctccg tggaagctca cggtccagct cagtgccacc tgggcgatcg
     1921 agacgccatg ggcgcgcgcg atggagcgaa gctcatcgag cagaggagcc gttcgcgcca
     1981 gccgcaaggg gctcatcgtc cccatcatcc gccgcatccg ggagaccttg gccacctggg
     2041 acggatcctc gtggaatctt cccgtgagga ctccctgcgc cagcggggag taggcaatga
     2101 tcgtgatccc cagccgcttg gcggcagcca ggacgccgtt gcgctcgatg tgacgatcca
     2161 ggagattgaa ccgcatctgg ttggaagcca gcacgagccc ttgggacgcc aacgtggcgt
     2221 gcgccagctc catggagcgg gcggagaagt tgctgactcc caccgcccgg atcctgtggt
     2281 ccagcagcag cttcgccatc tcacgcatct ggcccggcac gggagacacg ctccatggct
     2341 gatgcacctg gtagaggtcc acggggtagg gagccagaca ggcgatccgg gtctcgatgt
     2401 tggccgggat ggtcctggcg gaacggagga cgggccacca cttggtggca atgcgaacgc
     2461 tgccgggctt gacccccgcg gtcttgagcg ccgcggccag gcccttctcc gaggtccccc
     2521 ggccgtacag ctccgcggtg tcgaaccagt cgatgccgcc tttcagggcg gtggcgacca
     2581 cctcgtcgat ggtcctctga ttcagggttt cccagaagtt gctgaaccag ctcgagctct
     2641 gggagaactg ccagcagccc agcccgatgg gcgtgacttc tacgtctgtc tttccgatgc
     2701 gccgtttgtt cattgaggtt tctcctctag tcgaggatac agcccgaaag gtggtttggg
     2761 gaaatcgtcc gaaggggcgg gcgctcctgg cggcagcggg gaatcgcgta tgggcaccgc
     2821 ggatgaaagt ggcagccgga cggcgggttg atggcgctgg ggatctcgcc cttgggcagg
     2881 tgcttgtcgt gtcgggcggt ggggtccggg atgggaacgg ccgccaggag cgccttggtg
     2941 tagggatggt gcggtcggga gaagaggtca tcccgggagg ccacctccac gatcctgccc
     3001 aggtacatga cggcaatccg gtcacagagg tacttcgcgg tggccaggtc gtgcgtaatg
     3061 aagaggtagg tgagccccat ctcgagcttg agctgccgca tcagctggag gatgagggct
     3121 cggacggaaa catcggccat ggcgatgggc tcgtccgcca cgaggaactc cgggccggta
     3181 acgagggcgc gggcaagcac cacgcgctgg ctctggccgc cggagagctg gtgcgggtac
     3241 ttgcggtaga ggaaccgtgc cggcgccagc cccaccttct ccatgaggtc gatgacccgt
     3301 tccttgcgtt ccgacgacgt tccgatcccg tggatctcca gggggtggct gatggcctgg
     3361 ccgacgctca tccgcgggct cagggaggaa ttgggatcct ggaacacgat ctgcgctttt
     3421 gcgcgcaggt gctccatctc ccggggggag aaggaggtga tgtcccggcc gcggtactgg
     3481 atgctgccgg aggtgggctc ggtgaggcgc aggaggagcc gacccgtggt ggatttgcct
     3541 gaaccgctct ctccggcaag gcccaggacc tcgccctccc cgatatcgaa gctcacccga
     3601 tctaccgcgt gaacggccat gcgccggggg gagagcacgt cgaagatggt gccgcgaagc
     3661 ggaaagttct tgaccaggtc cttgacggaa atgagcgcgg ccatcagtcc ctcgttccgt
     3721 acaaccagca ggctgcatgg tgcgccggag cgtgcgaacg gagcggcggc tccttctggc
     3781 ggcagatcgg catgacccgt gcgcaccggg cggcgaaccg gcaccccggc ggaggctgga
     3841 ccagatccgg cggcatgccc ggcatggtgg tgaggtcctt ctggtccaac aggatgttgg
     3901 ggacacaggc gatcaggccc tgggtgtacg ggtgaagagg atgggcgaag atctccctgg
     3961 cggtcgcgac ctccatgatc ttcccgccgt acatgaccgc gatccggtcc gcgatctcgg
     4021 ccaccaggcc caggttgtgc gtgatcagga tgatggagag cttgtacagg tcccgaagcg
     4081 tggtgaggag gtccgtgaac ccggcctcca cgatcacgtc cagcgacgtg gtcggctcgt
     4141 ccgcgatcag gatgtccggg ttcatgatga gcgcgagccc gatcatgatc ctctggcgca
     4201 tcccgccgga gagctggtgg gggtactcgg caagccgctt tcgctcgatg cccagcgtct
     4261 ccaggatctt ctccacgccg gcccgcgcct ttgccttgct cataccggga tcatggaagt
     4321 ggatggtctc caggaagtgc cgctctaccg tgaaaagcgg gttcagggag gtgagcgggt
     4381 tctggaagat catggcgatg ttctgaccgc ggagcgcgag cagggcctgg ccgctcatac
     4441 ccaggatgtc gttgcctcgg tagcggatgg cgccggccgt cacccgaccg ggcggtcgaa
     4501 gcagcttgag gatggagaga cccagcgtgg acttgccgca accggattcg ccgaccagcc
     4561 ccagcgcctc gccgtccgcg agagtgatgg agacctcttc cacggcccga accacgccga
     4621 tcgagatggg gtaggcgatg gagaggtggt ccacctccag gatggtgggg ttgcccttgc
     4681 tggccgcggt ttccgtggtc tgtgcctggt gggtagggct catcgttcca acagcctcgg
     4741 gttgaggatt tccgccaatc cctctcccag catggtgaag cccagggcaa cgagcatgat
     4801 catcaggccg gggaacgtga tcatccacca gattccggag ggcaggaact tcttgcccat
     4861 ggagagatcc atgccccagt cggggatgcc cgccggaaga cccagaccga tgtaagacag
     4921 ggcggcctcc gtcatgatgg cgtccgcgat gttcagggac aggaccacga ctaccgttgc
     4981 gatgacgttc ggaaacacgt agcgccccag gatggtcccc cgccgggcgc cgatggcgcg
     5041 ggccgcctcg acgtagacct cctccttgat ggagagggtt tggccgcgga ccagtcggaa
     5101 gtacgtgggg atgtagacca cggccaccgc cagggtgatg ttgatcaccc cgggaccgag
     5161 catggcggcg aaggcgatgg ccaggatgag cccggggaac gagtacaccg aatccatgat
     5221 cagcgacagg atccggtcga agaggccgcc cgcgaacccg gacaccaggc ccagcgggat
     5281 gcccaccaga gcggacacga gcgcggcggc gatggcgacc cccaggatcg tctgcgcgcc
     5341 atagaccacg cgggcccaga cgtcccgact caggttgtcc gtgcccatga ggtgctcgcg
     5401 gctgggtggg gtcatctggc cgccggcgtt ctggtccagc gggctgtagt gggtgaacag
     5461 gtgggggaac accgtcatgg tcacgatcac ggcgatgatg aggacccccg cgatcatgat
     5521 ccaccactcc ccgccgtact tcctgcgcga tgagctgaac cagcggaaga cggaggccag
     5581 tcccgccgcc ggggagagtc gagcggtccg caccggttgc cgcgcgggct gtgtgctcat
     5641 gacgcgctcc tcaatacttc accctcgggt cgatgacggc atagatcaga tccaccagga
     5701 gcgagatgac gctcaccatc agcgtataga cgatgatgac cccctggatc acgggatagt
     5761 cccggaggta gatccgctcc aggaggagcc gacccatgcc cggccaggag aacgtgctct
     5821 ccgtcaggat ggcgcccgcc aggagcaggc tgaactggag gcccagcatg gtgacgatgg
     5881 ggatgagggc gttgcgaagc ccgtggttgt agacgacgcg ccgctccggg atgccccgcg
     5941 cccgcgccgc caggatatag tcgctcttca ggacgtccag catgttggcg cgggtgaggc
     6001 ggacgaagat ccccgagagc acgatcccca gcgtgaagga cggcaggacc aggtgccaca
     6061 gcacgtcccc gaagttggca acctgtcccg cgcgcagcgt atccaggacg tagaacccgc
     6121 ttcggctgaa ctccgatacg tcgatgcgcg gtcccgtgcg ccccgagacc gggaaccagc
     6181 ccaggaacac cccgaacacg agctggagga gcagccccat ccagaacacg ggaatgcagt
     6241 acgccacgtt ggcgtacagc cggatcccga aatcccttcc ggaccggcgc cgccgggcgg
     6301 ccgaggcgcc gagggggatg ccgatggcga ggctggccag gaggccgaac agggtcagct
     6361 cgatggtggc ggggagcttt tcgatgatgg gctggctgac cttctgctgg aagatcatgg
     6421 aggtgcccag gtcgaaatgg atcagctggt tcagatagtc gacgtactgg acggtgatgg
     6481 gacgattgag acccagggcc tcctttttct cgtcgatcac cttctgcggg gcatgaccgc
     6541 ccagcatggc gtttacggga tcccccggca tgatccgcag gaccacgaat accagggtca
     6601 ccaggatgaa gatcatgggg atcgaaagca ggagccgggt gatgaggtag ttcagcagat
     6661 ggcgcatgtc tggaaagaag gagggcccgg acccctcggg ggggccgggc cggatctcac
     6721 gggtctactt ggtgacgaac gaaagggtgt tgtagttgaa gatgagggta ggaccgatct
     6781 tcacacccgt cacgttcttg gtggtgaaca cgtacaggtt gccctggaag atgggcgccg
     6841 tggggacctc atccgtccac atctgctgca gcttggcgaa cacggagttg cgggttgcat
     6901 ccttcgtggt ctgctgctcc tggatgaaca ggtcgtccca ggccttgctc gagaagaaga
     6961 tcccgttgcc cttggagcct tcggtctggg cgaacggaga ggtgtagtcg tccgggtcga
     7021 tgtagtcggg gtaccacccc aggaagaaga ccggcatctg cttgttgttg aactgctgct
     7081 tgtaggtggc ccactcggcg gacttgatgg tcaccttcat gagcggcgtc ttctgcagct
     7141 cggcctggag gacttccgcc atgttgacct cggtgtcacc gtagtggctc ggggtgtacc
     7201 agaggtcgaa ggtgaagggg ttgtccgcgc tgtaaccggc ggccttgagg tccttctccg
     7261 ccagcgccac gttggcgtct cccagcaccg tcttgaaggc gtccgtgtgg tagctcatcc
     7321 ccacggggat catggagtac agcggggcgt tgtggcccag gtagaccttc tggttgatct
     7381 cgggccggtt gatgagggcc gcaaccgcct ggcgcagcct cttgtccttg aacacgctct
     7441 ccgaggtctc gaagcagagg tagcggatct gaggtccggg gatcttggtg ccgacgaact
     7501 tggggttctt cagcaggtcg tcgatatccg aggggttcag cgacttgaag gccaggtcga
     7561 tttccccgcg ctccagggcg agccgcatcg tcgtggcgtc cgcgaagtag cggatgatga
     7621 tcttcttgac cgccggttcc ttgccgaagt agttggggtt ggcctggaag acggcctgct
     7681 catcccgctt gaaggaggtg agctgatagg gtcccagggc cgcgatctcg ccgccgttga
     7741 gctcgctcac gtccttcacg atcttgtcct ccggatacac cttcggatcc atcgggaagt
     7801 aggtgggcgt tgcgaccagc tggggcaggt acgagagggg acccgtcagg ttgaagcgga
     7861 ccgtgtagtc gttgaccacg tcgacggact tcacgtactg ggtgaccagg gcggccgggt
     7921 cgccgttgag cttggccacg cggtcgatgc tccacttcac gacctgggcc gtgaacgggg
     7981 tgccgtcaga gaacttcacg cccttgcgga gcgtaaaggt atactgatct cccgcggggt
     8041 tggtggtgta ccccgtggcc agtccgggca ccaggtccgt cgtgccggga gtgtactgga
     8101 gcagcccgca gctcacgttc tggaagatct cccaggtatg gaagtcatag gcgctggcgg
     8161 ggtccatgtc ggtgaccttg tcggtggtcc cgtagaccag ggtctgcgcg aacagcgcag
     8221 aggccgacat cgccagagct gccacgagca ggatggtgaa cagtctcttc atactctctc
     8281 ctttccggta tagatagatg aaggtatggg agcggataca ccggtgtcaa tccctggaat
     8341 cgagcgagcg tccgctgcgg acgcggcgga ctgcgcggac ctgatcctgg cgtccgcgga
     8401 gcactttctc ccggccgtct tcggcccccg gatccgtggc gccctgatcc gcatggcggc
     8461 cggccgcggc acgctcttca gtcacgagca cgcgtggatg gcgcgggccg acggggccgc
     8521 ggccggcatc ctcctgggct acacggggct ccagaaggcc gcggaggatc ccgccaccgg
     8581 cctgaggctg ttcggcgctc tgggtccggg gctcctcctc cggctgagtc ggctcctgag
     8641 gctgcaacgc agcatcgggg cgctggcgcc ggacgagtac tacgtcagca acgtggccgt
     8701 ccgcccggag ttccgagggc aaggcatcgg cagcctgctc ctggagcgcg ctcgggcgga
     8761 agcagcggag gccggcgcgg cgtggatcgt tctggatgtg gagacggaca acgaaggtgc
     8821 ccgcagactg tacgagcggc tcgggttctc tcgtcggtcc gagacccggc cgctcgtgct
     8881 ggacggaacg ccgttttcct tctatcgaat gggccgaccc gtccgggatc cggactgaga
     8941 gcctactccc gcttcccgag ctggtgatcg agctggaaga tgcccgcgga gttcgccggg
     9001 attctcttca gcaggtccag gatccgggag gcgttcttct cctcctccac ctgctccgtg
     9061 atgaaccact ggatgaagac ctt
//
LOCUS       DASRZS010000553         5327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1088507, whole genome shotgun sequence.
ACCESSION   DASRZS010000553 DASRZS010000000
VERSION     DASRZS010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088507"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="VFH83_13415"
     CDS             <1..1668
                     /locus_tag="VFH83_13415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6487419.1"
                     /translation="ILLGVYVTRDVIALRKTVVSYGQGDFSARSSIKSRDEVGSLARS
                     FNTMIETITGYQDKLVAAEREKATAELASKVEGARNAENRKYLDNISQGLLMLDENLR
                     IGEQYSASLVRLFRYPGNPAGQGLLDFVYPDAEKNVTERAELTSFLDILINNTTADQD
                     MLDEINPIKDRELRAYDGSTIFVDARFLRIHREDAVENVMVMFEDKSGLREVEKKLTE
                     ERDRYDGELEAIAAILKNGPVLFKDFVAEGETLTAEFAASLGDLRDPERSASFMRQFH
                     SFKGLARSLQLGHLAQSAHRIEDLLLAARGDGGLVDGQPQALRQALDSMIEGIATIKQ
                     SVERFSAFQKQGDPTPRIELEASIRSFSDMVVQLAREMNKDVLFEPAVDVTELPFLKE
                     LKNPIIHLLRNAMDHGMEDLYERTASGKPAKGRIRLEIGKEGSEVRVAVRDDGKGIDF
                     ERIRRKAVQKGLLAEGNTATNDELLKILFLADFSTKDGVSEISGRGVGLDAVLHVVRQ
                     LGGTIKVTTTRGAGTCFLLTIPLKRASDASGRRAPAETAPARAALPS"
     gene            complement(1681..2142)
                     /locus_tag="VFH83_13420"
     CDS             complement(1681..2142)
                     /locus_tag="VFH83_13420"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HET6487420.1"
                     /translation="MKAEFINPFLKASINLFKEYLGLQAESGKPYLRTDPQNLDEVSA
                     IIGLAGDTRGAVVLSFSRETAIKVVSKMEGKEYRLLGNEVLDGVGELVNIIAGNAKQD
                     LLGFRIEISLPGVVTGKAYKIRWPDSIPVVTIPFTTDVGPFTVNVSLKDIA"
     gene            complement(2152..2523)
                     /locus_tag="VFH83_13425"
     CDS             complement(2152..2523)
                     /locus_tag="VFH83_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6487421.1"
                     /translation="MNILIVDDSGVMRRVHKGILLEKGIAEPDIFEAEDGKVALEVAT
                     SKPIGLFLVDWNMPKLNGLEFVRTLRGMDAYKTTPIVMITSEAAKYNVVEAVKAGVTS
                     YVIKPIKGEALWEKIGKYLQG"
     gene            complement(2624..3199)
                     /locus_tag="VFH83_13430"
     CDS             complement(2624..3199)
                     /locus_tag="VFH83_13430"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET6487422.1"
                     /translation="MTSGAVLMVLAIIATLVVSRRGILPFVAREERYIMATEAAMLSI
                     FVVELAVRLLSRHFSAPRLVERGTRLRLIVRIVGYSTALLSEVSILASNAALGISVGA
                     VVGVAIAFAAQNVVGGLLAAVLILGTRMVRVGEEIVVNQTRGIVSDISLTHTVLAVEE
                     DVVFIPNSLIISSMVRRRRRTIPGDAAVRDW"
     gene            3369..>5327
                     /locus_tag="VFH83_13435"
     CDS             3369..>5327
                     /locus_tag="VFH83_13435"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing transglutaminase
                     family protein"
                     /protein_id="HET6487423.1"
                     /translation="MRRWRHSLCASLLAAATLLVAPGVAVAEDFQRTFTIAPPPSWVL
                     PQGSLALPTHGADEGESYLLSDSQENVATGERFTNIVKEMRTAEGVQDGSTISVDYDP
                     SYQTLQFHRILVVRGGSASSRLSRRAVRLIQREPDLESSMLDGAVTASFVLQDIRPGD
                     RIDYAYTIRGRNPALGGRFADSFPYGWQTALGRERVRVLCPTSRVLRYRSIGGTAEPR
                     VTAKGSTTEYLWEFSDSAPILWEDQSPSWHVTYPWVEISEYPDWAAVDRWAQSLYPPA
                     KLPPELGKLTTRWSAEHAAAVDRLQAALDYVQQEVRYVAIELGTGSLTPRAPQAVWAR
                     RFGDCKDKSYLLVSLLSRLGLQAAPVLVNTGDRTLVADRLPSPYAFDHVIVAVTVNGK
                     RVFVDPTDAYQRGPVLKRFLPDYGYGLILAPGQTQLVRFGSAQGAQPETRVVERFTTA
                     GQDEPASLEVTTTASGGAADNLRAAFATHTIDEVAKSYLNYYASDYPQIESVARLDVR
                     DDAAQDVFTTVERYRIPGFWTLLSDKRNYRATLFPSFVYGLIPMPKTKIRTSPIEVPY
                     PKTVDERIEVSLPEAWPARSESATITTAAFELKTDYAARGTTVVLEHRYRSLADSIPA
                     AAVPGYNEAVKDIDQRLGYRLTWQAKGVQ"
BASE COUNT          938 a         1783 c         1712 g          894 t
ORIGIN      
        1 atcctgctgg gggtctatgt gacgcgggac gtcatcgcgc ttcgaaagac cgtggtgagc
       61 tacggtcagg gagacttctc cgcccgctcc tccatcaagt cccgcgacga ggtggggagc
      121 ctcgcgcgca gcttcaacac catgatcgag acgataacgg ggtaccagga caagctggtt
      181 gccgcggagc gggaaaaggc gaccgcggag ctggcgtcca aggtggaagg ggcgcgaaac
      241 gcggaaaacc gcaagtacct ggacaacatc tcccagggac tcctgatgct ggacgagaac
      301 cttcgcatcg gggagcagta ctccgcgtcc ctggtacggc tgttccgata cccggggaat
      361 ccggccgggc agggcctcct ggatttcgtc tacccggacg cggagaagaa cgtcacggag
      421 cgcgcggagc tcacctcctt cctggacatc ctgatcaaca acacgaccgc ggaccaggac
      481 atgctggatg agatcaaccc gatcaaggac cgggagcttc gagcctacga cggcagcacc
      541 atcttcgtgg acgcgcggtt cctgcgcatc catcgcgagg acgcggtgga aaacgtgatg
      601 gtcatgttcg aggacaagtc cgggctccgc gaggtcgaga agaagctgac cgaggagcgc
      661 gatcgctacg acggggagct ggaggccata gccgccatcc tcaagaacgg ccccgtactc
      721 ttcaaggact ttgtcgccga gggagagacg ttgacggcgg agttcgcggc ctccctcggg
      781 gatctgaggg atcccgagcg atctgcgtcg ttcatgcggc aattccattc gttcaagggg
      841 ctggcgcgat ccctccagct cggtcacctg gcccagtcgg cccaccgaat cgaggacctc
      901 ctgctggcgg ctcgtgggga cggcggcctg gtggacggac agccccaggc gctgcgtcag
      961 gcgctggact cgatgataga ggggattgct acgatcaagc agtccgtaga gcggttctcc
     1021 gccttccaga agcaggggga ccccacgcct cggatcgagc tggaggcctc catacggtcc
     1081 ttctccgaca tggtggtcca actggcgcgg gagatgaaca aggacgtcct gttcgagccc
     1141 gcggtggacg tgaccgagct gccgttcctc aaggagctga agaacccgat catccatctc
     1201 cttcgcaacg ccatggacca cgggatggag gacctgtacg agcggaccgc aagcggcaag
     1261 cccgcaaagg gcaggatccg gctggagatc ggcaaggagg gaagcgaggt ccgggtggcg
     1321 gtgcgggacg acgggaaggg gatcgacttc gagcggatcc gccgcaaggc ggtccagaag
     1381 ggactcctcg cggaggggaa caccgcgacc aacgacgagc ttctgaagat cctgttcctt
     1441 gcggacttca gcaccaagga tggagtgagc gagatctccg ggcgcggggt agggctggac
     1501 gccgtgctgc acgtggtccg gcagctcgga ggcacgatca aggtcaccac gacgcgcggc
     1561 gcgggtacct gcttcctcct caccatcccg ctgaaacgcg ccagcgatgc atccggccga
     1621 cgcgcgccgg cggagactgc accggcgcgg gcggcgctgc cctcctaggg gcacggctct
     1681 ctacgctatg tccttgaggg agacgttgac ggtgaaaggc ccgacgtccg tcgtaaacgg
     1741 gatggtcacc acggggatgc tgtccggcca acggatcttg taggccttcc cggtgacgac
     1801 gcccggcagt gagatctcga tgcggaaccc gaggaggtcc tgcttggcgt taccggcgat
     1861 gatgttgacc agctcgccga cgccgtccag gacctcgttt cccagcaggc ggtactcttt
     1921 gccttccatt ttggagacca ctttgatcgc cgtttcccgg gagaagctca ggacaacggc
     1981 gccccgggtg tccccggcca gaccgatgat cgcggagact tcatccagat tctggggatc
     2041 cgttcggagg taaggtttgc cgctctccgc ctgcaggccc aggtactcct tgaagaggtt
     2101 gatcgatgcc ttcaggaacg ggttgatgaa ctctgctttc acgggggctc cttacccttg
     2161 gaggtatttt ccgatctttt cccacagggc ctcccccttg atgggcttga tcacgtagct
     2221 ggtcacgccg gccttgacgg cttccaccac gttgtacttc gccgcctcgg aggtgatcat
     2281 gacgatcggc gtggtcttgt aggcatccat gcctcgcagc gtgcggacaa actccagccc
     2341 gttcagcttg ggcatattcc aatcgaccag gaagaggccg atgggcttgg aggtcgccac
     2401 ttccagcgcc accttcccgt cctccgcctc gaagatatcg ggctccgcta tgcccttttc
     2461 caggagaatc cctttgtgga cgcggcgcat gaccccggaa tcgtcgacga tcagaatgtt
     2521 catccatccc ccaaagaaga tagaatcggc cgcctgcggg accctcggta cgtgcggtgt
     2581 cgaaaagtat agcccgcggc gccagcctat ccaatcacgg atttcaccag tcgcggaccg
     2641 cggcgtctcc gggaatggtc cgccgcctgc gtcggaccat ggaggagatg atgagggagt
     2701 tcgggatgaa caccacgtcc tcctccacgg ccaggacggt atgggtgagg ctgatgtcgg
     2761 agacgatccc ccgggtctgg ttgacgacga tctcctctcc cacccgcacc atgcgggtcc
     2821 ccaggatcag caccgccgcc aggaggccgc ctaccacgtt ctgcgcggcg aacgcgatgg
     2881 ccacgcccac cacggcgccg acgctgatcc ccaacgcggc gttcgacgcc aggatggaaa
     2941 cctcggacag cagggcggtc gagtagccga cgatgcgcac gatgagacgc agccgcgtgc
     3001 cgcgctcgac cagccggggg gcgctgaagt gacgcgacag caggcggacc gccagctcca
     3061 ccacgaagat ggagagcatg gccgcctccg tggccatgat ataccgttcc tcgcgcgcca
     3121 cgaagggcag gatgccccgg cgactcacga ccagcgtggc gatgatggcc agcaccatga
     3181 ggaccgcgcc gctggtcacc gccctggcgg gccttccgcg cagggccgac cccctggtta
     3241 cgtctgcggt tttttctgac atgcttcccc aaccctattc tcgataggga cgggcgcaaa
     3301 ccttagggcg agcggaaccg ccgccgccgc gataagcttt tctgtgaatc acggtcacgg
     3361 aggctgcggt gcgtcgttgg cgccattctc tctgcgcctc gcttctcgcg gcggcgaccc
     3421 tgctcgtcgc tcccggcgtc gccgtggccg aggacttcca gcggacgttc accatcgccc
     3481 cacccccttc gtgggtcctt ccgcaaggga gccttgcgct accgacccac ggcgcggatg
     3541 agggcgagag ctacctcctc agcgactccc aggagaacgt ggcaacgggc gagcggttca
     3601 cgaacatcgt caaggagatg cggaccgcgg agggcgtcca ggacggctcc acgatctcgg
     3661 tcgactatga cccttcctac cagactctgc agttccaccg catcctcgtg gtccgcggcg
     3721 ggtccgcctc ctcgcgtctt tcccgccgcg cggtgcgcct catccagcgc gagccggacc
     3781 tcgagtcctc gatgctggac ggcgccgtca cggcgtcctt cgtgctgcag gatatccgcc
     3841 ccggcgatcg catcgactac gcctacacca tccgcggccg caaccccgcc ctgggaggcc
     3901 gcttcgcgga ctccttcccg tatggctggc agacggcgtt gggacgggag cgcgtgcgcg
     3961 tgctgtgccc cacgtcccgg gtcctccggt accgttccat cggcggaacc gcggagccgc
     4021 gggttacggc caaggggtcg acgaccgagt acctgtggga gttcagcgac tccgccccga
     4081 tcctgtggga ggaccaatcc ccgtcctggc acgtgaccta tccctgggtc gagatctccg
     4141 agtatccgga ctgggccgcc gtggatcgat gggcccaatc cctgtatccg cccgcaaagc
     4201 tgccgccgga gctcgggaag ctcacgaccc gctggtccgc ggagcacgcc gccgcggtgg
     4261 atcgtctgca ggcggccctg gactacgtcc agcaggaggt ccgctatgtc gcgatcgagc
     4321 tggggaccgg gtccctcacc ccgcgcgctc cccaggcggt gtgggcccgt cggttcggcg
     4381 actgcaagga caagtcctac cttctcgtca gcctgctctc gcgtctgggg ctgcaagccg
     4441 ccccggtcct cgtgaacacc ggcgaccgta cgctggtggc ggaccggctg ccttccccct
     4501 acgccttcga tcacgtgatc gtggcggtca ccgtgaacgg gaagcgggtc ttcgtggatc
     4561 ccacggacgc ctaccagcgc ggacccgtac tgaagcgttt cctcccggac tacgggtacg
     4621 gcctgatcct ggcgcccggc cagacccaac tcgtgcggtt cgggtcggcc cagggagcgc
     4681 agccggagac cagggtggtg gagcgcttca ccacggccgg tcaggatgag ccggcttccc
     4741 tggaggtgac caccacggca tccggtggag ccgcggacaa ccttcgcgcc gcctttgcca
     4801 cgcacacgat cgacgaggtg gccaagagct acctcaacta ctatgcctcc gactatccgc
     4861 agatcgagtc cgttgcccgg ctcgacgtcc gggacgatgc agcccaggac gtgttcacga
     4921 ccgtcgagcg ctatcggatc cccggcttct ggacgctcct gtcggacaag cgcaactacc
     4981 gggccactct ctttccctcc ttcgtgtacg ggcttatccc gatgccgaaa acgaagatcc
     5041 gaacgtctcc catcgaggtg ccatacccca agaccgtgga cgaacggatc gaggtgagcc
     5101 tgcccgaggc ctggcctgcg cgatcggaga gcgccaccat caccacggcc gccttcgagc
     5161 tcaagacgga ctacgccgct cgaggcacga cggtcgtcct ggagcatcgc taccgctccc
     5221 tcgccgactc catccccgcg gccgcggtcc cgggctacaa cgaggcagtc aaggacatcg
     5281 accagagact gggctatcgg ctcacctggc aggccaaggg cgtccag
//
LOCUS       DASRZS010000554         8294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1470505, whole genome shotgun sequence.
ACCESSION   DASRZS010000554 DASRZS010000000
VERSION     DASRZS010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8294
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470505"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VFH83_13440"
     CDS             complement(<1..969)
                     /locus_tag="VFH83_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487424.1"
                     /translation="MREPLKLPFIHLLFILSSTILLALLDLCWSWGVSQAGAHRFTFT
                     YFLEQFPSSAFSVIVPAVVISTLLIGFRMARHPFSRLLGMVLVLAVSYLVMVNGMIWL
                     GRLSSEARTTEAIPRQFVQPRTLVRLGSTVLAANTVAGADLGPTLVANPSRTSARLTV
                     YPGGTIIVKDGTLAVRLTGGPSSPALSGAPLSTASSVFVPDRITAFFLRDIHTLTSDY
                     HRLLDRSLPQFFAACFALLFLCSASLMLLRVTRWPLANVVLLAAAVRGYFSLYHLLAV
                     DLAPQVAQAVADPLLVRLFPEAVMAGLAVVLLLVDILFVPARRAEER"
     gene            complement(992..1573)
                     /locus_tag="VFH83_13445"
     CDS             complement(992..1573)
                     /locus_tag="VFH83_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6487425.1"
                     /translation="MERIRASLRRSPFVFLGSDEPLAPECADLYVAPVGRVGDLPRHG
                     LPVIAAGPAEMLRSAFLAGCADYLREPWTAEELSLRALAAMARGARRFTFPWGELALE
                     SDRLHTAAGPVRLTWHESRILAALLRARGEPVPRQALAYTAGVSSITTSRARSASRAI
                     DAHVSSIRRKVRAVLPQADRFIVCVRSQGYLIP"
     gene            complement(1626..2333)
                     /locus_tag="VFH83_13450"
     CDS             complement(1626..2333)
                     /locus_tag="VFH83_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HET6487426.1"
                     /translation="MGILDRIRTVLKANINALISRAEDPEKMLNQLIVDMNEQLLDAK
                     KQVAMAIADEKKLERQTLENKGQAEDWERKAMLAVKAGKDDLAKEALLRKQEYEGYAA
                     SFQKEFESQHASVEQLKDALRQLQSKIEEASRKKNLLIARAKRAEAQKQIQQTMGSLA
                     GGSSFDTFDRMAQKVDQIEAEADAMKELGTVTADQKLEDKFKELESSDATADKLLEDL
                     KSKMGQLENKESGHGTP"
     gene            complement(2346..2735)
                     /locus_tag="VFH83_13455"
     CDS             complement(2346..2735)
                     /locus_tag="VFH83_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487427.1"
                     /translation="MISSDKIESYLVKLSLSFQQVAAGTWVVRDAAKGIENLLIILSD
                     PLVVLRLTVMEVPPKGREKLFEELLRLNASDMVHGAYAVDGRHVIIVDTLEADAMDLS
                     EFEASIDAIGLAVAQHYRVLSTYRVKA"
     gene            complement(2746..3297)
                     /locus_tag="VFH83_13460"
     CDS             complement(2746..3297)
                     /locus_tag="VFH83_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6487428.1"
                     /translation="MSNNDIVTGFDDEKDDSLKIRLQKIEDTEGCLVLYLTGYIDTYN
                     SNYFQKRVAKAIEGGFIKLIFHCSGLNYVSSTGIGSFTAFLKAVKPRGGDLVLLEIQP
                     KVYEVFQLLGFSQFFNIKENLDEAVSFFAARGEEGKGAVFPRIFKCPICSKKLKASKA
                     GRFRCSECKTILAIDNAGQVFLG"
     gene            complement(3476..4138)
                     /locus_tag="VFH83_13465"
     CDS             complement(3476..4138)
                     /locus_tag="VFH83_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487429.1"
                     /translation="MKCFRKNVMLLCALLACGPAWCMDWKLPILSVRYEAASGETEDP
                     DEEGLLPASLRQTFALQMKEEVAPAVFDLSLTYSLKDYFLQNGDYSYLCLAQGGAVRL
                     GAVKLAYDLGAKSTEYPLPDSDGLPKDLVSLRAGTAATFFLVKGTSLETSLAGRFDLA
                     ENQAKSAQAYVGTLALTSRLGDWQVAGRLRGELRLPLGDTSLKDRSTYAVGSLTASWD
                     PN"
     gene            4161..4922
                     /locus_tag="VFH83_13470"
     CDS             4161..4922
                     /locus_tag="VFH83_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET6487430.1"
                     /translation="MGTQREVRIVLVRPKDARNVGACCRAMKCMGFTSLAIVPEEPLD
                     PEQARTLAHYATDVLENARMFPDLPSALTDAVLVAGTTRRRGKNRKYFTLFPEQLGER
                     IAGIASGTVAVVFGNEDTGLTDEELRLCHLAVTIPASPQFPSLNLSHAVQVICYEIRR
                     VLEESRQTPFTPIQGDALDGLVSVITRTLGGLGFFTRVGPESMTVFARDILARAGLSV
                     NEARRIEVTFRKIAGLAGRRLRERAEGIDPHPDRS"
     gene            4945..5424
                     /locus_tag="VFH83_13475"
     CDS             4945..5424
                     /locus_tag="VFH83_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487431.1"
                     /translation="MSRSLPLLACTLLVLAAPVAIGAPQGDFDRVVAFDITLKSLASD
                     AASGAPLPNRVIILDGTVSDVNVVDKNAATFKVRIQMIAGEWIGLDDVKSYTCYVDFS
                     GSQYASVFPARPPAAAKSGVVTLNSRVLVVGRPVSLVSAPDGSPTVLVEGMHVRSLE"
     gene            complement(5445..6575)
                     /locus_tag="VFH83_13480"
     CDS             complement(5445..6575)
                     /locus_tag="VFH83_13480"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6487432.1"
                     /translation="MVRVLFIDDDPNEHRTLAHVLPDPYTLLSAYTGREGLEVNAREG
                     PDLVLLDINLPDLDGIQVLKAIATRPAAPPVVMLTGHEEPRVVKEAILGGACDYVVKP
                     FDLAELVGTLRAAVTGIDARRAAVHGSQETVLTELIGESAGMRETRQLILRYAPSGSP
                     VLVIGESGTGKELVARAIHQLSRRRTGPYVALNCGALPDTLLETELFGAERGAYTDAV
                     TRPGSFEQANGGTLFLDEIGEFSASAQTRLLRVLEQKELRRVGATRSVPLDVRVVSAT
                     NRELKSEIRLGRFREDLYYRLGVLPIRLPPLRDRREDIPMLALHFLAALGRPMTRIAS
                     DAVEQLLAYPWPGNIRELRSVMERSALNATEETIRARDLSFD"
     gene            6886..7407
                     /locus_tag="VFH83_13485"
     CDS             6886..7407
                     /locus_tag="VFH83_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487433.1"
                     /translation="MGKMEHLGLVRRLPFHGGGRRPLLYIRPGCVQSPPSFRGTLVDA
                     VIRGLLPMLVDPEFSWWKTGRIRQIDLLAEGRLESVGFCFSSNLNTLNRDWWPLRLAL
                     RRGLIHRGFLLHPGERAFFASAKVFALPAAEFVADLRAWILGRESNCVAIRAMDRIND
                     RSVRRYWKRAPLL"
     gene            7422..8174
                     /locus_tag="VFH83_13490"
     CDS             7422..8174
                     /locus_tag="VFH83_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET6487434.1"
                     /translation="MIALTKRSQALVPNESGYLVWNYRSEKVTTDPRRLAVVICDMWD
                     RNWSRAANERAEPLARQIDRFITRARAAGMTVIHAPSETMKFYEGSPARVRVLAIEQA
                     PFPVKRPHEDPPLPIDHSDGGSDTNLGNEKIHTAVWTRQCEAIHIDEERDYISDDGAE
                     IHSLMRARGVDTVLLCGVHTNMCILNRSFAIKSFASRGIDIVLLRDLTDALYNPGKSP
                     YVSHDEGTALVIGYIEKFWCPTATSAEILSGL"
BASE COUNT         1431 a         2682 c         2730 g         1451 t
ORIGIN      
        1 ccgctcctcg gcccttctcg ccgggacgaa gaggatgtcc acgagcagga gcaccacggc
       61 cagacccgcc atgacggcct ccgggaagag gcgcacgagg agcggatcgg ccacggcctg
      121 ggccacctgc ggcgccagat ccacggcaag caggtggtac agggagaagt acccgcggac
      181 cgcggccgcc agaaggacca cgttggccag cggccagcgc gtgactcgaa gcagcatcag
      241 ggacgccgaa cagaggaaca ggagcgcgaa gcaggccgcg aagaactggg gcagggaccg
      301 atccagcaac cggtggtagt ccgatgtcag cgtgtggatg tcccggagga agaacgcggt
      361 gatgcgatcc ggcacgaaca cggagctggc cgtggataac ggcgcgccgg agagcgcggg
      421 agaagacggg cctccggtca accggacggc gagcgtcccg tccttcacga tgatcgtgcc
      481 gcccggatat acggtgagac gcgccgaggt ccgcgagggg ttcgcgacca gcgtcgggcc
      541 gaggtcagcg ccggcgaccg tgttggccgc gagcaccgtg gaaccgagcc tgacgagcgt
      601 ccggggctgc acgaactgcc gcggaatggc ctccgttgtc cgcgcctcgg aagacagacg
      661 tcccagccag atcatcccgt tcaccatcac gagatagctg accgcgagga ccagcaccat
      721 gccgaggagt cgggagaacg gatgccgcgc cattcgaaac ccgatcagca gcgtggagat
      781 caccaccgcg gggacgatca cggagaacgc gctggacggg aactgctcca ggaagtacgt
      841 gaacgtgaag cggtgagcgc cggcctggga cacgccccac gaccagcaga gatccagcag
      901 ggccaggagg atcgtcgagc tcaggatgaa gaggagatgg atgaagggca gcttcagcgg
      961 ctctctcacc gccccccagc gtaccacggg ttcacggaat aaggtagccc tgggagcgga
     1021 cgcagacgat gaaccggtcg gcttggggga ggacggcgcg caccttgcgg cggatggagg
     1081 acacgtgcgc gtcgatggca cggctggcgg aacgcgcgcg cgaggtcgtg atggaagaga
     1141 cgcccgcggt gtacgccagg gcctgccgcg gcacgggttc cccgcgcgcg cggaggagcg
     1201 cggcgaggat ccgtgactcg tgccacgtca ggcggacggg accggcggcg gtatggagcc
     1261 ggtccgattc cagagccagc tcgccccacg ggaacgtgaa gcgccgcgct cctcgcgcca
     1321 tcgcggccag ggcccgcagg gacagctcct ccgccgtcca gggctcccgc aggtagtcgg
     1381 cgcagccggc caggaacgcg gagcgcagca tctccgcggg ccccgccgcg atcaccggca
     1441 gcccgtgccg cggcaggtcc ccaacccgcc ccaccggcgc cacgtagagg tccgcgcact
     1501 ccggcgccag cggctcgtcg ctccccagaa aaacaaacgg gcttctccga agagaagccc
     1561 gtatccgttc caccaccaga gcatcgagac ctacgaggtt gatcctccgc tcacctggcc
     1621 gaacgtcacg gagtaccgtg accggactct ttgttctcaa gctggcccat cttggacttg
     1681 aggtcctcca gcagcttgtc cgcggtggcg tcggaggact ccagctcctt gaacttgtcc
     1741 tccagcttct ggtccgcggt cacggtgccc agctccttca tggcgtcggc ctccgcctcg
     1801 atctggtcca ccttctgggc catccggtcg aacgtgtcga acgaggagcc tccggccagg
     1861 gatcccatgg tctgctggat ctgcttctgc gcctccgcgc gcttggcgcg ggcgatcagg
     1921 aggttcttct tgcggcttgc ctcctcgatc ttgctctgga gctggcgcag cgcgtccttg
     1981 agctgctcca ccgacgcgtg ttgactttcg aactctttct ggaacgaggc ggcgtacccc
     2041 tcgtactcct gcttgcgcag cagggcttcc ttggccaggt catccttgcc cgccttcacg
     2101 gcgagcatgg ccttgcgctc ccaatcctcg gcctggccct tgttctccag cgtctggcgc
     2161 tcgagcttct tctcgtccgc gatggccatg gccacctgct tcttggcgtc caggagctgt
     2221 tcgttcatat cgacgatcag ctggttgagc atcttttcgg gatcctcagc ccggctgatg
     2281 agcgcgttga tgtttgcctt gaggacggtg cggatgcgat caagaatccc catgggctcc
     2341 tcctgctacg ccttgacccg gtaggtagaa agtacgcggt agtgctgggc cacggcaagc
     2401 ccgatggcgt cgatggaagc ctcgaactcc gagagatcca tcgcgtcggc ttccagcgtg
     2461 tccacgatga tgacgtgtct cccgtcgacg gcgtaggcgc cgtggaccat gtccgaggcg
     2521 ttcaggcgca ggagctcctc gaagagcttc tccctgccct tgggcggcac ttccatcacc
     2581 gtgagcctga ggacgaccag gggatccgag aggatgatca ggaggttctc gatgcccttc
     2641 gccgcgtccc ggaccaccca cgtgcccgcg gctacctgct ggaagctgag cgacagtttc
     2701 accagatagc tttcgatctt gtcgctcgag atcatggggt cctcctcagc cgaggaacac
     2761 ctggcctgca ttgtcgatgg cgaggatggt cttgcactcc gagcagcgga accgccccgc
     2821 tttggacgcc ttcagcttct tgctgcagat ggggcatttg aagatgcgag ggaacaccgc
     2881 ccccttgcct tcctcaccgc gggcggcgaa gaaggagacc gcctcgtcca ggttctcctt
     2941 gatgttgaag aactgggaga agcccaggag ctggaacact tcgtagacct tgggctggat
     3001 ctccaggagg accaggtcgc cgccccgagg tttcaccgcc ttgaggaacg ccgtgaagga
     3061 accgatgccc gtgctgctaa cgtagttgag gccgctgcag tggaagatca acttgatgaa
     3121 gccgccctcg atggccttgg caacgcgctt ctggaagtag ttggagttgt aggtgtcgat
     3181 gtagccggtg agatagagga ccagacaccc ttcggtatcc tcgatcttct ggaggcgaat
     3241 cttgaggctg tcgtcctttt cgtcgtcgaa tccggtcact atatcgttgt tactcatggc
     3301 gcccctttgc ccacaaagat tcctcggccc atccaactat aaccccggcg tgagaatgcc
     3361 gcaagggctg atgcgcgtgg tgcttcccag ataatgatcg gaaagtgtaa ggagtttcat
     3421 cagttttgtc aaatccaacg gtggattggg gtgttcgacg ggcattttcg gagtttcagt
     3481 tgggatccca gctcgccgtg agacttccca cggcgtacgt ggatcggtcc ttgaggctcg
     3541 tgtcccccag aggcaggcgc agctcgcccc gcaacctccc cgccacctgc cagtctccca
     3601 gtcggctggt cagggccagc gtgccgacgt aggcctgcgc cgacttggcc tggttctccg
     3661 ccaggtcgaa ccgcccggcc agggaagtct ccaggctggt ccccttgacg aggaagaagg
     3721 tggccgcggt gccggcccgc agggacacca ggtccttggg gagcccgtcg ctgtccggga
     3781 ggggatactc ggtggacttg gcccccagat cgtaggccag cttgaccgct cccagccgca
     3841 cggctccccc ttgggccagg cataggtagg agtagtctcc gttctggagg aagtagtcct
     3901 tgagtgaata cgtcaggctg aggtcgaaga ccgcaggcgc cacttcctct ttcatctgca
     3961 gtgcgaaggt ctgccgaagg gacgccggca ggagcccctc ttcatcgggg tcttccgtct
     4021 ccccactggc tgcttcgtag cgaacgctga ggatgggaag cttccagtcc atgcaccagg
     4081 cggggccgca ggccagcagg gcacagagga gcatcacgtt tttcctgaaa cacttcatac
     4141 tatagtagta tagtgcgccg atgggcaccc aacgagaagt tcggatcgtc ctggtccgac
     4201 cgaaggacgc gcgcaacgta ggcgcgtgct gtcgggccat gaagtgcatg gggttcacat
     4261 ccctggctat cgtgcccgag gagccgctgg atcccgagca ggccaggacc ctggcacact
     4321 acgccaccga cgtgctggag aacgcccgca tgttcccgga cctgccgtca gccttaacgg
     4381 acgccgtgct ggtggcgggc acgacacggc ggagaggcaa gaaccgaaag tacttcacct
     4441 tgttccccga gcagcttggg gagcgcatcg ccggcatcgc cagcgggacc gttgccgtgg
     4501 tcttcggcaa cgaggacacc ggcctcacgg acgaggagct gcgcctctgc catctggcgg
     4561 tcaccatccc ggcctctccg cagtttcctt ccctgaacct gtcccacgcc gtgcaggtga
     4621 tctgctacga gatccgccgt gtcctcgagg agagccgcca gacgccgttc acgccgatcc
     4681 agggagatgc tctggacggg ctggtctccg tgatcacgcg cacgttgggc ggtctgggct
     4741 ttttcacccg ggtcgggccg gagtccatga cggtgttcgc gcgggacatc ctggcccggg
     4801 ccggcctctc cgtgaacgag gcgcgccgga tcgaggtcac cttccgcaag atcgctggcc
     4861 tggccggcag acggcttcgg gagcgggccg agggaattga cccgcatccg gatcgatcgt
     4921 aggatgaccg aaaggagcct ggcgatgtcg agatccctgc cgctgctcgc gtgcaccctc
     4981 ctggtcctgg ccgctcccgt ggccatcggc gcgccccagg gcgacttcga ccgggtcgtc
     5041 gccttcgaca tcaccctgaa gagcctggcg tcggatgccg cgagcggagc tccgctcccc
     5101 aaccgggtca tcatcctgga tggcacggtc tccgacgtga acgtcgtcga caagaacgcc
     5161 gccacgttca aggtgcgaat ccagatgatc gccggagagt ggatcggtct ggacgacgtg
     5221 aagagctaca cctgctacgt ggacttcagc ggctcccagt acgccagcgt gtttcccgcc
     5281 cgccctcccg cggcggccaa gtctggagtg gtgacgctca actcccgcgt gctcgtggta
     5341 ggccgaccgg tgagcctcgt gtccgctccg gacggatcgc ccaccgtcct ggtggagggg
     5401 atgcacgtcc gctccctgga gtagggtctc ttctgccccg cggatcagtc gaaggagaga
     5461 tccctggccc ggatcgtctc ctccgttgcg ttcagcgccg agcgctccat cacgctccgc
     5521 agctcacgga tgttgccggg ccatgggtac gccaggagct gctcgaccgc gtccgatgcg
     5581 atcctcgtca tgggtctgcc cagcgctgcc aggaagtgga gagccagcat cgggatgtct
     5641 tcccgtcgat cccgcagggg cggtagccgg atgggaagga cgccgagccg atagtagagg
     5701 tcctctcgga acctccccag gcggatctcc gacttcagct cccggttcgt ggcggagacc
     5761 acccggacgt ccaggggcac cgagcgggtc gcgcccacgc ggcgaagctc cttctgctcc
     5821 agcacccgaa ggagccgggt ctgagcggag gcggagaact ccccgatctc atcgagaaac
     5881 agcgtgccgc cgttggcctg ctcgaagctg cccggtcgcg tgaccgcgtc cgtgtacgcg
     5941 ccgcgctcgg ctccgaagag ctccgtctcc aggagcgtgt ccggaagggc cccgcagttg
     6001 agggcaacgt agggaccggt ccgacggcga gagagctggt ggatggcgcg ggccaccagc
     6061 tccttgccgg ttccgctctc tccgatcacc aggacggggg agcccgacgg cgcgtaccgt
     6121 aggatgagct gacgggtctc gcgcatgccg gcgctctcac cgatgagctc cgtgagcacg
     6181 gtctcctggg agccatggac ggccgcgcgg cgcgcatcga tgccggtgac ggccgcgcgc
     6241 agcgttccga cgagctccgc gaggtcgaac ggcttcacca cgtagtcgca ggcgccgccg
     6301 aggatggcct ccttcaccac gcgcggctct tcgtggccgg tcaacatcac cacgggcggg
     6361 gcggccggcc gggtcgcgat ggccttgagc acctggatcc cgtccaggtc cggaaggttg
     6421 atatccagga gaacgaggtc cgggccttcc cgggcgttca cctccaggcc ttcgcggccg
     6481 gtgtatgcgg acagtagggt gtacggatcg ggaaggacgt gggccagagt acgatgttca
     6541 ttgggatcgt cgtcgatgaa gagcactctt accatggaag aatcactttg tttctggtga
     6601 gatcataccg ccggcgatcc gccggcgcaa gcccgcccgc gtcggtccgc ggtggcccgc
     6661 gttcagcctc gggttttcca gtgaggatag ctctaccctc aggaatccgc gcaaagcgca
     6721 agcaaaacca acgatttgtg cgttttcctg agggtgaagt gtggtagtca gaaatcctgg
     6781 tttcaggcgc ttgcggcagt cttgagtttc tgggacggca ggccgctgaa caccgcggag
     6841 atcggccggt tgcttggggt ttgccctttc accgtggatg cctggatggg gaagatggaa
     6901 cacctcggtc tggtccggcg gctgccgttt cacggcggcg gcagaaggcc cctgctgtac
     6961 attcggcccg gctgcgttca aagcccgccc agcttccgcg ggacgctcgt agacgcggtt
     7021 attcgggggc tgcttcctat gttggtcgac ccagagttct cgtggtggaa gaccggccgg
     7081 attcgccaga tcgacctgct cgccgagggc cgattggaat cggttggctt ctgcttctcc
     7141 agcaacctga acacgctcaa tcgggactgg tggccgctcc gcctagcgct tcgcagagga
     7201 ctgattcacc gcggcttcct cctgcatccc ggtgagcgcg cgtttttcgc atcggcgaag
     7261 gtgttcgctt tgccggccgc tgagttcgtc gcggatctcc gtgcgtggat cttgggccgt
     7321 gaatcgaact gcgtcgcgat aagagcgatg gatcgcatca acgaccggtc ggtccgtcgg
     7381 tactggaagc gcgcccccct cctgtgacac aatccgcgct catgattgca ctcaccaagc
     7441 ggagccaggc gctcgtcccg aacgagagcg gctatctggt ctggaactat cgcagcgaga
     7501 aggtgacaac ggatcccaga cggctggcgg tcgttatctg cgacatgtgg gaccgcaact
     7561 ggagtcgggc ggccaacgag cgcgccgagc cgcttgcccg tcagatcgac cggttcatca
     7621 cccgggcccg agccgcgggc atgacggtga tccacgcccc ttcggaaacc atgaagttct
     7681 acgaagggtc tcccgcccgc gtgcgggtcc tggccatcga gcaggctccg ttccccgtga
     7741 agcggccgca cgaggatccg ccgctgccca tcgaccactc cgatggcgga agcgacacga
     7801 acctgggaaa cgagaagatc cacaccgccg tctggacccg acagtgtgag gcaatccaca
     7861 tcgacgagga gcgggactac atctcggacg acggagcgga gatccatagt ctgatgcggg
     7921 cccgaggcgt cgacacggtc ctcctgtgcg gcgtgcacac caacatgtgc atcctgaacc
     7981 gctccttcgc catcaagagc ttcgcgtccc gaggcatcga catcgtgctg cttcgcgacc
     8041 tgaccgacgc cctctacaat ccgggcaaga gcccgtacgt gagccatgac gagggcacgg
     8101 ccctggtgat cgggtacatc gagaagttct ggtgtccaac ggcaacgagc gcggagatcc
     8161 tctcgggcct gtaggggtga ggcaggccgg cgcttagcgc cagtcgatga gcgcaaccat
     8221 tgcgttcggg tcccaggcca ggatgcggtc ataggcctcc ggggcccggc gccacggcac
     8281 gcgctcggtg atca
//
LOCUS       DASRZS010000555         2987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1089431, whole genome shotgun sequence.
ACCESSION   DASRZS010000555 DASRZS010000000
VERSION     DASRZS010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089431"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VFH83_13495"
     CDS             complement(<1..567)
                     /locus_tag="VFH83_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487435.1"
                     /translation="MLHFPILTFWNEEDLYKLQVSAVSRFMEVDTPYAVEASIEFARY
                     LCARGLDSDNYPLFLELLRKENGYVIEALIGGEKAFDLFTKVQPNPYILDFCLSTLRS
                     YAPGGVHDLTLEMVFGILYRAYHSARQGNALHTLTIEDVNAVGKFLDKSRDQNDAINR
                     FILDILADIAEHRSAHNEDPALDKVSTHA"
     gene            complement(945..1877)
                     /locus_tag="VFH83_13500"
     CDS             complement(945..1877)
                     /locus_tag="VFH83_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487436.1"
                     /translation="MGKGKGVLTKVTSYRRVGKKRLTSRSYRRRTRSALLSLARGAVL
                     CNTPEAVDASAALAQVVSMSGMNNRNYLLFLLLLETNHPLVIDVLIGPRNPFLLFSPI
                     KPNWYLLKETFRILAKFKGDELNVNALLALLGVVQLAYKTSKDGYKIYPLRMSDVYSI
                     GKHLDKARDQLDPRNRLLLEILFDIYFVGIDSDNRGAKQIGIKANDIRMAFFDNTKKM
                     LDAIPDVLLVKDLVRAALPPRFYGWEPPPAVVHPLEAREEPELKVGLASTRTEEEKGK
                     TRSRGASKGTGSTRTGSTRGGSTRGGPRRRKPRA"
     gene            1966..>2987
                     /locus_tag="VFH83_13505"
     CDS             1966..>2987
                     /locus_tag="VFH83_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487437.1"
                     /translation="MPRAPRLSSRLDSLLDAVFRTVADPRALFEEITEQLSPSQLAQA
                     QKRIRKTLREERELGPEAAVITACVLHCTGFPAHRILRFLSLYVPDTVPPSLFRLAVD
                     VIFRGQQPIAESLLYDLTQYPYLEKRVLARRSGEIAERLKPDLGPELLLFLYLQALRD
                     DLSNENVRLIAMVLRESAPSLVLRTRVAGVTADESDEVVRAVETVETRTLDPTVEGPT
                     GERAHRPDARPFDRHSASVFLDKYFSDEALAKMREVAPPPPVTKPVQSRASPRQSREI
                     AVEVRTAGKLPPVRTRRAPVPETPLETGEAARTAADSSPRVRVRSSAPTRANAAPRKA
                     SARRVKA"
BASE COUNT          525 a          960 c          961 g          541 t
ORIGIN      
        1 ggcatgcgtg gagaccttgt ccagggccgg gtcctcgttg tgcgcggagc ggtgttccgc
       61 gatgtcggcc aggatgtcca ggatgaagcg gttgatcgcg tcgttctggt cccgggattt
      121 gtccaggaac ttgcccaccg cgttcacgtc ctcgatggtg agggtgtgca gcgcgttgcc
      181 ctgccgggcg ctgtggtagg cgcggtacag gatcccgaac accatctcca gcgtgaggtc
      241 gtgcacgcca ccaggggcgt acgaccgcag cgtcgagagg cagaagtcca ggatgtacgg
      301 gttgggctgg accttcgtga aaaggtcgaa tgccttctcc ccgccgatca acgcctcgat
      361 gacgtagccg ttctccttgc gcaggagctc gaggaacagc gggtagttgt cgctgtccag
      421 gccccgcgcg cagaggtacc tcgcgaactc gatggacgcc tctaccgcgt agggcgtgtc
      481 gacctccatg aagcgcgaga ccgcgctcac ctggagcttg tacaggtctt cctcgttcca
      541 gaatgtcagg atcggaaaat gaagcatctc cctctctcca actggactcg gcactcctgg
      601 gcccggttcc cttccccact cacggtggtg gacgaccatt agaaagcaag ggtcatgcca
      661 accgccgccg cgaaatgcaa agaacctgga ccaagaaaaa aggctatgat tccccgcgtt
      721 caggcgatcc cagggaccgc gaacgcacta ttctctaact taccgagaga gcgacgcctg
      781 tatcaatatg gaacttcttg ttccacgctg gtggaacact cgcaattcta tgtatcgtga
      841 cagccgctgt caaacatttt gcgagagtcc gtggccagcg gtcgacacgg tctctcggga
      901 accggacacg ccgaacgcct gaaagcgccc gctgcccggc atgatcaggc cctgggcttg
      961 cgccgccgcg gtccgccccg ggttgatccg ccccgggttg atccggtgcg ggttgatccg
     1021 gtgccctttg acgcgccgcg ggagcgggtc ttgcccttct cttcctcagt ccgcgtcgaa
     1081 gcgaggccga ccttgagctc cggctcctct cgggcctcca ggggatgcac caccgcgggg
     1141 gggggctccc agccgtagaa ccgcggcggc agggcggccc gaacgaggtc cttcaccagc
     1201 aggacatcgg ggatggcgtc cagcatcttc ttggtgttgt cgaagaacgc catgcggatg
     1261 tcgttggcct tgatcccgat ctgcttcgcg cctcggttgt cgctgtcgat gcccacgaag
     1321 tagatgtcga acaggatctc cagcaggagg cggtttcggg gatcaagctg gtcgcgcgcc
     1381 ttgtccaggt gcttgccgat gctgtacacg tccgacatcc gcagcgggta gatcttgtag
     1441 ccgtccttgg acgtcttgta ggcgagctgc accactccga gcagcgccag cagggcgttg
     1501 acgttcagct cgtccccctt gaacttggcg aggatgcgga acgtctcctt gagcaagtac
     1561 cagttgggct tgatcgggga aaacagcaga aacgggttgc gcgggccgat aagaacatcg
     1621 atgaccagcg gatggttggt ctccaggagc aggagaaaga ggaggtagtt gcgattgttc
     1681 atgccggaca tgctgaccac ctgcgcaagc gccgcggagg catccaccgc ctccggcgtg
     1741 ttgcacaaca ccgcgccgcg ggcaagcgac agcaacgcgc tgcgggtacg gcggcggtag
     1801 gagcgggacg tgaggcgctt ttttccgacg cgacggtaac tggtgacctt ggtcagcaca
     1861 cccttgcctt tacccaattc tcggacatga gggcgttaat catgaccctg gggcgttttt
     1921 cttgacaatc cggcggcgaa acggtagggt cgcgccataa agcagatgcc acgagcgcca
     1981 cggctctcct cgcgcctcga ctcgctcttg gatgcggtgt ttcggaccgt ggcggatccc
     2041 cgggccctct tcgaggagat cacggagcag ctcagtcctt cccagctcgc ccaggcccag
     2101 aagagaatcc gcaagaccct gagagaagag cgggaactgg gtcccgaggc ggcggtcatc
     2161 accgcgtgcg tgctgcactg cacgggtttc cctgcgcatc gcatcctgcg cttcctgtcc
     2221 ctgtacgtgc cggacaccgt tccgccctcc ctgttcaggc tcgccgtgga cgtgatcttc
     2281 cgcggccagc agcccatcgc ggaatccctc ctctacgacc tcacccaata cccgtacctg
     2341 gagaagcgcg tcctggcccg caggagcggg gagatcgcgg aaagactgaa gccggacctt
     2401 ggccccgagc tcctgttgtt tctctacctc caggcgctcc gcgatgacct cagcaacgag
     2461 aacgtgcggc tcatcgccat ggtgctccgg gagagcgccc cttccctcgt ccttcgcacg
     2521 cgtgttgccg gtgtgacggc ggacgaatcc gacgaggtgg tccgtgccgt ggaaaccgtg
     2581 gagacccgta cgctcgatcc cacggtggaa ggccccaccg gagaacgagc ccacaggccg
     2641 gacgcgcggc cattcgaccg gcattccgcc tctgtgttcc tggacaagta tttctcggac
     2701 gaggcgctgg cgaagatgcg ggaagtggcg ccacctccgc ccgtcacgaa gccggtccag
     2761 tcccgggcga gcccgcggca gtcgcgcgag atcgccgtgg aggtgcggac cgcgggcaag
     2821 ctgccgccgg tgcgcacgcg cagggccccg gttcccgaga caccgctgga aacgggggag
     2881 gctgcccgca cggctgcaga ttccagcccc cgcgtccgtg ttcgttcaag cgcgcccacc
     2941 agggcgaatg ccgcgccacg gaaggcatcc gcgcggcggg taaaggc
//
LOCUS       DASRZS010000556        17483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5313471, whole genome shotgun sequence.
ACCESSION   DASRZS010000556 DASRZS010000000
VERSION     DASRZS010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 17483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17483
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5313471"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VFH83_13510"
     CDS             <1..802
                     /locus_tag="VFH83_13510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6487438.1"
                     /translation="QIITNLAVNARDAMPRGGTLLLETAALDVGENGAVESAEGRGWS
                     LPAGISRGPWVALRVRDTGVGMSEEVKSHLFEPFFTTKDEGKGSGLGLSTVYGIATQS
                     GGHVTVESELDRGSVFAIYLPRIEPAQVGPAPEPAPIAFRSGSGTILVVEDDDTLGAL
                     ARRVLESAGYTVILAASAPEALRCFEQTGRLDLVVTDIVMPGGMNGVELGSRLRVARP
                     TLPVLYMSGYTDDALIPSDGLGGHLPFLPKPFQPEDLLRQVAELLSPR"
     gene            909..1817
                     /locus_tag="VFH83_13515"
     CDS             909..1817
                     /locus_tag="VFH83_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6487439.1"
                     /translation="MEKRTLGKTGLSVTTLGHGAMEVRGARIWNGRPVTDAQAERILN
                     AVLDCGINFIDTSYDYGMSEEYIGRFLSRRRGEFYLATKCGCTLVNKGDHDDTPHVWT
                     RENLLHNIETSLKRMKTDHVDLWQLHNPTVEETRAGDLVAVLQEVKKSGKVRHIGISS
                     TLPHISQYIAMGVFESFQIPYSALERRHEEVIGKAHQSGAGVIVRGGVARGEPEKGLG
                     AAERWTLFSKARLDDLLAPGEDRTGFLLRFTLTHPGAHTVIVGTLNPDHLERNAKAVQ
                     AGRLSPETYAEARKRLADAGEKPEAV"
     gene            1923..2948
                     /locus_tag="VFH83_13520"
     CDS             1923..2948
                     /locus_tag="VFH83_13520"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET6487440.1"
                     /translation="MSDSNASVNPGEMIKWYRSPIPKDTLRSLTQRSDAMGFLQAGGY
                     LVVLAGTAVASFAAYRWLPLWAFFLVLFVHGTVWAFLTNGFHELVHGTVFRSKPLNAI
                     FLRIYSFLGWASPVQFRASHVRHHSSTLHPPWDEEVVLPIRLRVRNFLLSAIIDPIGL
                     YNTLRLFLRWSVGIYKGHWELVLFPAEDRAGRRALARWARIVLIGHAIIVAASIASGY
                     WLFAVVISGARFYGGALQWLCNNTQHIGLQDNVPDFRLCCRTVRLNPILQFLYFHMNY
                     HAEHHMYAAVPCYKLGRLRRTIASDLPDASPNLLAAWQEIIGILRRQEKEPGYQHVFK
                     LPVRQAA"
     gene            2958..3917
                     /locus_tag="VFH83_13525"
     CDS             2958..3917
                     /locus_tag="VFH83_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009544438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6487441.1"
                     /translation="MKTVPLGSTGRQVSALCLGAMNFGTKLDEKGSWQLLDRYYEAGG
                     RFIDTANNYAVWWGGNGTESENVLGRWMRDRRNRGDLFLATKVGFNTPTVGNGLSRRL
                     IRQEIEGSLRRLGTDHVELYYAHKDHRADALEETLATFDELHREGKVQTIGCSNYRAW
                     RIEEARALSRARGWIGYCCVQQRHTYLRPANGASFEPQIASDEELLDYGRSHAADFLV
                     LGYSSTLGGAYDGRSDRPIPSQYQGPDTDARLAALQAVASQTGATPVQVVYAWMLRSA
                     PLVLPLVSAGSLQQLDENLGALRVSLDDAQMKKLDEAGAWGNR"
     gene            3914..4855
                     /locus_tag="VFH83_13530"
     CDS             3914..4855
                     /locus_tag="VFH83_13530"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6487442.1"
                     /translation="MNRRDAVLGLVAGGGAPKGYVPAAFFLHFDADHHRGQAAVDRHL
                     EYYRATGMDLVKIQYEHPFPRLEQVRQPRDWTRIPRYGADYYAPPLEVVDGLVKAAKK
                     EAPVVLTLYSPLMLAAQATSRELVTEHLDQDPESVKKGLEIIAESILVLVRECARRGL
                     DGFYASTQGGESGRFKDKGTFARYIKPVDLMVMTEINRLCSFNILHVCDYHGVYEDLS
                     PYVDYPGSIVNCGTRLTSGPIQPAAIARMFDRPFLGGLDRHGVLANGDPTGIRREVLR
                     SAPPRFMLGADCTVPSETPWDNLKAAIAEAHGETGRA"
     gene            5113..5475
                     /locus_tag="VFH83_13535"
     CDS             5113..5475
                     /locus_tag="VFH83_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6487443.1"
                     /translation="MDKKVLIVDDANFIRMIVKNILVPNGFEIVGEATNGDEAVRQYE
                     RLKPDLVTMDITMREKNGLQAAKEIIAKDPNARIIMITALGQERMLVDSFKIGVRDFV
                     VKPFDPARILSAARKALA"
     gene            5486..5998
                     /locus_tag="VFH83_13540"
     CDS             5486..5998
                     /locus_tag="VFH83_13540"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HET6487444.1"
                     /translation="MAISADFVNPFIEAAMTVASKVAGITMRRGHLAYKRQPDPSYGV
                     SIIIGVYGFLKGQVVYSMKRELADRIVHNMLQDAPILDRDVLYLDALGELANMITGNA
                     TAILNQRKELAIGITTPAVAAGSNLTVTLINMPTLALGLYSQYGPIEINIALKETAPS
                     GDPNSETLMP"
     gene            6030..8882
                     /locus_tag="VFH83_13545"
     CDS             6030..8882
                     /locus_tag="VFH83_13545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET6487445.1"
                     /translation="MNPDYKALFLEESQGQIKQWEDALLVLEKSPEDQEMLHELLRAI
                     HTLKGSAGFVGCEEMRNLAHKLESAMVPEKDEDPRLLPGVIDLLFDGLDVVRRIIDAI
                     DVGDPVEGTARALLDRLDERLGTPAAAQAAPQPTAPRGPLYRINIHIEAPTAERYLRA
                     LLVQTRLEEIGTIVAVNPTLEELRLQDAEFEYGVVVETDRPPAALEAALNIDQVHLQR
                     IQPVGTPGEEAESGGAKEGPDPAAETGGATAKAENVVRVPASKLDAMLNLVGELVVQN
                     SGFLSLARDLRAAYGGSALVGSLEEKTEMLTRVARQLQDAVMKVRMLPAAAVFSRFPR
                     VVRDLAKSSGKEIQLQIAGGETEIDKKVIDRVSEPLVHLVRNAADHGIENATDRQAAG
                     KDPSGLIRISAYQEGDRLCLEVSDDGRGLDRSRILAKAIERGLVKEADAQAMSEDEVT
                     GLVFLPGFSTADRITDISGRGVGLDVVKKFVDEMGGAIRVRTRPGLGTTMTISLPLTM
                     AIIRAILVETSRLLYAIPISSVSEVVMVEPGAYTMVRGERVILLREEMLPVVRLRDLL
                     VGSAVQKLPEDAPQAGPGPVVVVQYGGHRVGLIVSTVIGNTEVVVKSLGRHYREIEGL
                     LGASILGNGRMAIILDVGVLLEMHFKGTQRALPSFQASAAAARASDAAQPSVVTPPPL
                     REPAPARPAAPEPTRVSEPIRSPDPARELEPAREPEPAREPEPSLPVELADPSAVVET
                     SGSDSPQGGLKDLEEVFSSSALRASQALTDLLGESIRVSFPETRFVPLTEVANRLGGE
                     DVPVIRVYVDVREGLTGGHLLIVPVDRAMPLCDRLLGRTAGATTDIRDDELSSVAELG
                     NILSAAFINSIADRMGISIHSDVPDVRLDMCLSTIDSLLARFQEPGSDILLTVAEVTC
                     GESEQIVCHMMLFLERPSLALMRDALTGRVTPTAER"
     gene            8893..9378
                     /locus_tag="VFH83_13550"
     CDS             8893..9378
                     /locus_tag="VFH83_13550"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HET6487446.1"
                     /translation="MEERTVVIRIAEMSVVRSDEQVRYTLKTTLGSCVGVILTDPVKD
                     IHGLAHVMLPQRLDGDQAVGKYADTAIPALVTEMERSGSNRKDIEALLVGGASMFQNE
                     QTPTLARIGAKNVEASARVLQRMGIRVVFEDTGGSAGRRVAFDGRGRPEVKTIDVRKL
                     P"
     gene            9375..10217
                     /locus_tag="VFH83_13555"
     CDS             9375..10217
                     /locus_tag="VFH83_13555"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HET6487447.1"
                     /translation="MNPSAVLALPVLTDDLFARFCILIQRETGICMRESKKLLVSNRL
                     SKRMRQIGIGSFQEYYDRVTGSDAAERVRFIDAVTTNETYFYRGENHFSILRRHILPE
                     RFAGKDRVTVWCVGCSTGEEAYTLAIEGMEAAGPGRFLEVTATDISTAVLAQAQAAVY
                     GGRTLRYLPEVIRGRYFDAAGGEEYRVKEEVRRRVRFLQHNALHEEPPVRGVDVIFCR
                     NVMIYFDLPTQVRLVDERFASALAAEGYLFIGHSESLIGKSRRFSYSRIEHAPVYRLR
                     KDPS"
     gene            10214..11284
                     /locus_tag="VFH83_13560"
     CDS             10214..11284
                     /locus_tag="VFH83_13560"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007038999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HET6487448.1"
                     /translation="MIRVMVVDDSPLVRKIVTDILSAQPDIGVCATAPSGELALHKLE
                     RSQPDVVTMDISMPGMGGLAAIRQIMGSRPTPVIVLSAFAHRGAEHTLQALEAGAVDF
                     VLKPSVSISGGVAQVARELVDKIRQAAQINLGLLAPPPPAPGAADPRGEAAAPTPALL
                     RPVEIVAIGASTGGPAALKVVLQSLPEDLPVGVVVVQHMPVLFTRPFAEHLDAICRVA
                     VKEAEHGDEIVDGRVLVAPGDRHMTVTREGGRPRVLLNQDEPVGGHRPSVDVLVRSVA
                     REYGCRAIGVIMTGMGKDGAQGIGELRDQGGYVIAQDSATSVVFGMNGEVVRRGAAHA
                     VVPVSAIAGAILSRIHSPEAAG"
     gene            11303..11614
                     /locus_tag="VFH83_13565"
     CDS             11303..11614
                     /locus_tag="VFH83_13565"
                     /inference="COORDINATES: protein motif:HMM:NF022179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HET6487449.1"
                     /translation="MNIAASYDLEILQNRGMEMVLKRAGSFLDEHGEFCRCEQCVFDL
                     LAFTLNHVTPLYATSLLGALSGNSRLQQKLEIEIEMALEEGAKRIARHPSHLDAGDTG
                     S"
     gene            11640..12128
                     /locus_tag="VFH83_13570"
     CDS             11640..12128
                     /locus_tag="VFH83_13570"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET6487450.1"
                     /translation="MEGTKGRKAADAAMDQMISFSVGTEQYGVDIRKVREVILPGEIT
                     PLPRAPDFVKGMINLRGDVIPVIDLREKLGRAGGGRPGEDRIIVVEISEKLIGMIVDA
                     VSHVLRVAPGQLEPTPPWLGGLTGEYITGVVRQTGGLIVVLNVDTILTSGEKLALENM
                     TR"
     gene            12154..14205
                     /locus_tag="VFH83_13575"
     CDS             12154..14205
                     /locus_tag="VFH83_13575"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6487451.1"
                     /translation="MSARVKVWMKVAGGFAILIVLIIAVTMIAYVQNRSVAKAVASAE
                     ALMGNSAPQDMMVRIIEILQARMNEGQYGLQGGNLKDVADQVKSLNTMVADLLKGGDL
                     NASQRERLQKMAQSAETFRSDYMAYWVERGNEARYASDWSDARDRLDAAAGHSGNLAE
                     AFDQLQVTALTFLKDKQPLAWDAYTAAQADFTSTASRAKRGDAALMAAADEYAKTMAS
                     YKTYFEKESAASVTMTKTVNDILSTSRDLERELTQIQQNSSATAILLMFIALALAVLV
                     GVGIALIITRGITVPVTRAVQFARTIAQGDLTQRLAIEQKDEIGELASSLDQMAGKLG
                     EMVTTIQDSAEQVAASSEQISASSVNLTEGAQGQASTLEETSASVEELAASIDQVSSH
                     AASQAAAFEQGAGSMAQVQKSIEDISLNLGKIADMARMSLDRSQEGAQAVDQVVEAIT
                     LIAQGSERIAGIVSMITDIANQTNLLALNASIEAARAGEYGRGFAVVADEVSKLAARS
                     ATSAKEIETLIQESVKNVTVGVDRAKGSKVSMEQIMETARFMSDMIHNVSTAMQQQVS
                     GVRELSKALENISEMAQSISAASEEQSASAKEVSRAVEKGNDLTQQTASSTEQISASA
                     EQLSGMASQLQSLISTFKVARNGSGKIEATSKAPEAAALPKAEPAPLEPVSGPVVPSH
                     P"
     gene            14285..15409
                     /locus_tag="VFH83_13580"
     CDS             14285..15409
                     /locus_tag="VFH83_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487452.1"
                     /translation="MGSKTIGAAAFLLLTLASVAMAAGKAEANGWVGTTKPCFTCHGS
                     GSSGANLLGARQEYDVSGHKTRGNSIYANGGGCQVCHTNEGFIQSCEGQQITASSFVA
                     TPSQPGCFTCHDPHERGDLSLRTVAPVKLASGATFDSGNGNLCATCHHATAAARDLVV
                     PTAANTITPSWGAHQSPQADIVEGTNLYEFPGKKYSSSAHKDVIPDGCLGCHMALPRG
                     RYGFNSSVGGHSFTIVGQVEGAVSVNTAGCISCHKDLKAVPGSDAFDIIAQEDYDHNG
                     VKEPVQVEVAGLLSVLVNAQGTGLLQRLDPPFYKSDGSFNEVTSTAKRPALEIAAFYN
                     YRMIADDRSRGIHNAKYVIQVLYDTIHALNPQFDVSRRPQ"
     gene            15630..15779
                     /locus_tag="VFH83_13585"
     CDS             15630..15779
                     /locus_tag="VFH83_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487453.1"
                     /translation="MLVAVTVDRSIQIRFPAPTSTYEALGSFSATVCALIFVLRLAMK
                     SDGSP"
     gene            complement(15780..17144)
                     /locus_tag="VFH83_13590"
     CDS             complement(15780..17144)
                     /locus_tag="VFH83_13590"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET6487454.1"
                     /translation="MNGHEKDGMSLPARILLYGGLPASFLALVLVTSFLWGCAGSSST
                     GPSMAIKGSFAKVPRALSGLTFNHKYHTVDVGLSCDACHEQNAANPRLVSFPDHDTCA
                     ACHQDEIDANGPKKNCELCHTQRDYKTRVRKNVVLSPLVVFDHPQHQKSGVDCSQCHA
                     AFDKDVLTADEMLPSMDACVSCHTDRKVKGANDCYSCHVKGLEKLTPQTHTASWKLSH
                     GAGLTRDLIASNCTVCHTKELGTSCTECHHQPPLSSGKSVSCSVCHGDGFDTTRPADH
                     TPGWVSGHGKGLTQTRIDQDCSLCHTVANGNDCQTCHRREAPPTHTIGWAQSVHGVAA
                     RTDRQSCDVCHDQSQCIACHSTNQPFTHTGSWGSPWNRHCLSCHMEGGGYVSGSMEGN
                     CGVCHNATDVFARHLSSSFPTPNPPTVPAHTTGLVCTTCHTVQNVQHPMPRNGAACVT
                     CHTH"
     gene            complement(17157..>17483)
                     /locus_tag="VFH83_13595"
     CDS             complement(17157..>17483)
                     /locus_tag="VFH83_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487455.1"
                     /translation="VVGGEIGRKDGPFDLRLGSSFNASLYQTDYTQTVLEDSFYDQEY
                     YLKVKWQISRFFDLSLSAAYENVLLSSITSAQPLNPDVDSVAMSGLDNAARNYFRFEV
                     RAGFRY"
BASE COUNT         3105 a         5623 c         5929 g         2826 t
ORIGIN      
        1 gcagatcatc accaacctgg ccgtaaacgc ccgagacgcc atgccgcgcg gagggacgct
       61 gctcctggag accgcggcgc tggatgtcgg agagaacggc gccgtggaat ccgccgaggg
      121 acggggctgg tctctgcccg cgggcatctc ccgcggaccc tgggtcgcgc tgcgcgtccg
      181 ggataccggc gtggggatgt ccgaggaggt gaagtctcac ctcttcgaac cgttcttcac
      241 gaccaaggac gagggcaaag gctccggact gggtctgtcc accgtgtatg ggatcgccac
      301 ccaaagcggc ggtcacgtca ccgtggagag cgagctcgat cgaggaagcg tctttgccat
      361 ctacctgcct cggatcgagc ccgcgcaggt cggacctgcc ccggagcctg ccccgattgc
      421 gttccgatcc ggcagcggca ccattctcgt cgtcgaggac gacgataccc ttggcgcgtt
      481 ggcccgccgc gtcctggagt cggctggtta cacggtcatc ctggcagcgt cggcgcccga
      541 ggcgctgcgg tgcttcgagc agacgggccg gctggacctc gtggtcacgg acatcgtgat
      601 gcccggcggg atgaacggcg tagagctggg ctcccgactg cgcgttgccc gaccgacgct
      661 gccggtcctc tacatgtccg ggtacacgga tgacgccttg attccgtccg acggtctggg
      721 cggccatctt ccgtttctcc cgaagccgtt ccagcccgag gacctcctgc ggcaggtggc
      781 cgagctcctg agtccccgct aggagcctgc cggacctccc cgtcctcacg cctccctctg
      841 ccatccttgg cccaggttgc caccgaccgg agccgctgcg taccttctct ggagacggag
      901 gaggacatat ggaaaaacgg acgctgggca agacgggact gtccgtcacg accctggggc
      961 atggcgcaat ggaagtccgg ggggcgcgga tctggaacgg tagacccgtc acggatgcgc
     1021 aggcggagcg gatcctgaac gccgtgctgg actgcgggat caacttcatc gatacctcgt
     1081 acgactacgg catgagcgag gagtacatcg gccggttcct cagccgacgc cgcggggagt
     1141 tctacctggc gaccaagtgc gggtgcaccc tcgtcaacaa gggggaccac gacgacaccc
     1201 cgcacgtttg gacgcgggag aacctgctgc acaacatcga gacctcgctc aagcggatga
     1261 agacggacca cgtggatctg tggcagctcc acaaccccac cgtggaggag acccgggccg
     1321 gtgacctggt ggcggtgctc caggaggtga agaagtcggg caaggtccga cacatcggca
     1381 tctcctccac gctgccgcac atcagccagt acatcgccat gggcgtgttc gaatccttcc
     1441 agatcccgta ctccgccctg gagcgccgcc acgaagaggt gatcggcaag gcgcatcagt
     1501 ccggggctgg agtgatcgtt cgaggcggcg ttgcccgagg ggagcccgag aaggggttgg
     1561 gagcggcgga gcgatggacc ctcttctcca aggcccgtct ggacgacctg ctggctcccg
     1621 gagaggatcg aacgggattc ctgctgaggt tcaccctgac ccatccgggg gctcatacgg
     1681 tcatcgtagg cacgctgaac ccggaccacc tggagcggaa cgccaaggcg gtgcaggccg
     1741 gccgtctctc gccggagacc tacgcggaag cccgcaagcg ccttgccgac gcgggggaga
     1801 aacccgaggc ggtgtagtcc gcccggaggc gcatggtctt ccggaggcgg gtcgcctccg
     1861 gctgggatgt agtatcttag ccccgagccc gctttctcgg cgcgggccca ggaaggtagg
     1921 caatgagcga ctccaacgca tccgtcaacc cgggtgagat gatcaagtgg tatcgatccc
     1981 ccatccccaa ggacacgttg cgctccctga cccagcgcag cgacgccatg ggttttctgc
     2041 aggccggcgg ttacctggta gtgctcgcgg gcaccgcggt ggccagcttc gcggcgtacc
     2101 ggtggcttcc gctctgggcc ttcttcctcg tgctctttgt ccacggtacc gtgtgggctt
     2161 tcctcaccaa cggatttcac gagctggtcc acggaaccgt attccggtcc aagcccctga
     2221 acgccatctt cctcaggatc tacagcttcc tgggttgggc aagccccgtg cagttccggg
     2281 ccagccatgt ccgccaccat tccagcaccc ttcatccgcc ctgggatgag gaggtggtcc
     2341 tgcccatccg actgcgggtc cgcaacttcc tgctgagcgc catcatcgat cctatcggcc
     2401 tctacaatac cctgcggctc ttcctgcggt ggagcgtggg gatctacaag ggccactggg
     2461 agctggtcct gttccctgca gaggaccggg cggggcgccg ggcgctggcg cgctgggccc
     2521 gcatcgttct gatcgggcac gccatcatcg tagcagcctc catcgccagc gggtactggc
     2581 tcttcgccgt tgtgatcagc ggagcgcgct tctacggcgg cgcgctgcag tggctgtgca
     2641 acaacaccca gcatatcggt ctccaggaca acgtccccga cttccggctc tgctgccgga
     2701 cggtccgcct caaccccatc ctccagttcc tttacttcca tatgaactat cacgccgagc
     2761 accacatgta cgccgcggtt ccttgctaca agctgggccg gttgcgccga accattgcgt
     2821 ccgacctgcc ggatgcctcg ccgaacctgc tcgccgcctg gcaggagatc atcgggatcc
     2881 tccgtcgcca ggagaaggag cccggctacc agcacgtgtt caagctgccc gtgcggcagg
     2941 cggcgtaggg gcgtcccgtg aagaccgtgc cgctgggttc cacgggcaga caggtcagcg
     3001 cgctctgtct cggcgccatg aacttcggga cgaagctcga tgagaaaggg tcgtggcagc
     3061 tcctggaccg gtactacgag gcggggggca ggttcatcga caccgcgaac aactacgccg
     3121 tatggtgggg cgggaatggc acggagtccg agaacgtgct gggccgctgg atgcgcgacc
     3181 ggaggaaccg gggagacctc ttcctggcta ccaaggtggg tttcaacacg cccacggtgg
     3241 gcaacgggct ttcccgccgg ctcatccgcc aggagatcga ggggagtctg cgtcggctgg
     3301 ggaccgacca cgtagagctg tactatgctc acaaagatca ccgcgcggat gccctcgagg
     3361 agaccctggc gaccttcgac gagctgcatc gggaggggaa ggtccagacc atcgggtgca
     3421 gcaactaccg cgcctggcgt atcgaggaag cccgcgcgct gagccgggcg cgcggctgga
     3481 tcgggtactg ctgcgtccag cagagacaca cgtatctgcg gccggcgaac ggggcgagct
     3541 tcgagccgca gatcgccagc gacgaggagc tcttggatta cggcaggtct cacgccgctg
     3601 atttcctggt gctggggtat tcctccacgt tgggtggggc gtatgacggc cgttcggatc
     3661 ggcccatccc ctcccaatac cagggcccgg acacggacgc ccgtctggcg gcgcttcaag
     3721 cggtggcgtc gcagaccgga gcaacgccgg tgcaggtggt gtacgcgtgg atgctccggt
     3781 ccgcgccgct ggttcttccg ctggtgagcg ccgggtccct ccagcagttg gacgaaaacc
     3841 ttggcgccct gcgggtgagc ctggatgacg cgcagatgaa gaagctggat gaggctgggg
     3901 catgggggaa cagatgaaca gacgggacgc ggttctgggt ttggtggccg gcggaggagc
     3961 cccgaagggt tacgtgccgg cggcgttctt ccttcacttc gacgcggatc accaccgggg
     4021 ccaggccgcc gtggaccgtc acctggaata ctaccgcgcc acgggcatgg acctcgtgaa
     4081 gatccagtac gagcacccct ttccgcggct ggagcaggtc cgacagccca gggactggac
     4141 ccggattccg cgttacggag ccgactacta cgcgcctccg ctggaggtcg ttgatggcct
     4201 ggtgaaggcg gccaagaagg aggctcccgt agtcctcacc ctctactcgc cgctcatgct
     4261 cgccgcccag gccaccagcc gggagctggt aacggagcac ctggaccagg accccgagag
     4321 cgtgaagaag ggtctggaga tcatcgccga gagcatcctg gtgctggtgc gggagtgcgc
     4381 ccggcggggc ctggacgggt tctacgcgtc cacccagggt ggggagagcg gccggttcaa
     4441 ggacaaggga acctttgcgc gctacatcaa gccggtggac ctcatggtca tgacggaaat
     4501 caaccgtctg tgctcgttca acatcctcca cgtctgcgac taccacggcg tgtacgagga
     4561 cctgtcgccc tacgtcgact atcccggatc gatcgtgaac tgcgggaccc gcctgacctc
     4621 cgggcccatc cagcccgcag ccatcgcccg gatgttcgac cggccctttc ttggcgggct
     4681 ggatcgccac ggcgtgctgg ccaacgggga cccgacgggg atccggcggg aggtgctgcg
     4741 gagcgcaccg cctcggttca tgctcggcgc cgactgcacg gtcccctccg agaccccgtg
     4801 ggacaacctg aaagcagcga tcgcggaagc ccacggcgaa accggcaggg cctgaaaacc
     4861 gcgttttttt tggcgatccc ccgaactccg cgctatactt ccggatcgag agactttcct
     4921 aactagaacg gatttccttt ctgctgccgc gaaagccctc gtcaagccct ggaggttgag
     4981 aacgaatgtg gcgggccgtc ttccagccgc acgctctgcg attgccctca cgccaacggg
     5041 gcgaggcaag gacggcgggg ggagggtctc gcgcagccgg ttcctgcgcg cacgttggtc
     5101 aaggagcgca ccatggataa gaaggtcctg atcgtggacg acgccaactt catccggatg
     5161 atcgtcaaga acatcctcgt acccaacggg ttcgagatcg tgggagaggc gacgaacggg
     5221 gatgaggcgg tccgtcagta cgagcggctg aagccggacc tcgtcaccat ggacatcacc
     5281 atgcgcgaga agaacgggct ccaggcggcc aaggagatca tcgccaagga ccccaacgcg
     5341 cggatcatca tgatcacggc gctgggccag gaacggatgc tcgtggactc gttcaagatc
     5401 ggggttcggg acttcgtggt caagccgttc gatcccgccc ggatcctctc cgcggcgcgc
     5461 aaggccctgg cgtagggagg ccgggatggc catcagcgct gacttcgtca atccgttcat
     5521 cgaggcggcg atgaccgtgg cctccaaggt cgctgggatc accatgcgcc gcgggcacct
     5581 cgcctacaag cggcagccgg accccagcta tggcgtctcc atcatcatcg gggtctacgg
     5641 gttcctgaag ggccaggtcg tctacagcat gaagcgcgag ctggcggatc ggatcgtcca
     5701 caacatgctc caggacgcgc ccatcctgga ccgggacgtg ctctacctgg acgccctggg
     5761 ggagctcgcc aacatgatca cgggcaacgc caccgccatc ctgaaccaga ggaaagagct
     5821 ggccatcggg atcaccaccc ccgcggtggc cgcggggtcc aacctcacgg taaccctcat
     5881 caacatgccg acgctcgcgc tggggttgta cagccagtac ggtcccatcg agatcaatat
     5941 cgccctcaag gagacggctc cgagcggaga cccgaactcg gagacgctca tgccatgacg
     6001 gcgcgcggag caagcgtaga gagggatcca tgaaccccga ctacaaggcc ctgttcctcg
     6061 aagagtctca aggtcagatc aagcaatggg aggacgccct cctggtgctg gaaaagagtc
     6121 cggaagacca ggagatgctc cacgagctgc ttcgcgccat ccacacgctg aaaggctccg
     6181 cggggttcgt gggctgcgag gagatgcgga acctggcgca caagctggag tccgccatgg
     6241 tcccggagaa ggacgaggac ccccggctcc tgccgggcgt catcgacctc ctgttcgacg
     6301 ggctggacgt ggtgcgccgg atcatcgacg ccatcgatgt cggtgatccg gtggagggca
     6361 cggcgcgagc gctcctggac cgcctggacg agcggctggg gacgccggcg gcggcgcagg
     6421 ccgcgccgca gcccaccgcg ccgcgggggc cgctgtatcg gatcaacatc catatcgaag
     6481 cgcccaccgc cgaacgatac ctgcgggcgc tgctggtcca gacccgcctg gaggagatcg
     6541 gtaccattgt cgccgtgaac cccacgctgg aggagctgcg tctccaggac gccgagttcg
     6601 aatacggcgt cgtggtcgag acggatcggc ctccggcggc gctggaagcc gcgctaaaca
     6661 tcgaccaggt tcacctccag cggatccagc cggtgggcac gccgggtgag gaagcggaga
     6721 gcggcggcgc caaggagggc ccggaccccg ccgccgagac cggcggcgcc accgcgaagg
     6781 cggagaacgt ggtccgcgta ccggcctcca agctggatgc catgctcaac ctcgtgggag
     6841 agctggtggt gcagaactcg gggttcctgt ccctggcgcg cgatctgcgc gccgcgtacg
     6901 ggggctcggc gctggtaggc agcctggagg agaagacgga gatgctgacg cgcgtggcgc
     6961 ggcagctcca ggatgcggtg atgaaggtcc gcatgctgcc cgccgccgcc gtgttcagcc
     7021 ggttcccacg cgtggtgcgg gacctggcca agagcagcgg caaggagatc cagctccaga
     7081 tcgccggcgg cgagacggag atcgacaaga aggtcatcga ccgggtcagc gagcccctgg
     7141 tccacctggt ccgcaacgca gcggaccatg gcatcgaaaa cgcaacggat cgacaggcgg
     7201 ccggcaagga cccatcgggg ctcattcgca tctccgcgta tcaggaggga gaccgcctct
     7261 gtctggaggt ctccgacgac ggccgcgggc tggaccgcag ccggatcctg gccaaggcca
     7321 tcgagcgtgg tctggtgaag gaagccgatg cccaggccat gagcgaggac gaggtcacgg
     7381 gcctcgtgtt cctgcccggc ttcagcacgg cggaccggat caccgatatc tcgggccggg
     7441 gggtcgggct ggatgtcgta aagaagttcg tggacgagat gggaggcgcc atccgcgtcc
     7501 gcacccgccc gggcctgggc accaccatga ccatttccct ccccctgacg atggccatca
     7561 tccgggcgat tctcgtcgag acatccaggc tcctctacgc gattccgatc tcctccgtca
     7621 gcgaggtcgt aatggtggag ccgggcgcct acacgatggt ccgaggagag cgcgtgatcc
     7681 tgctgcggga ggagatgctc ccggtcgtaa ggctccgcga tctgctggtg ggcagcgcgg
     7741 tccagaagct cccggaggac gctccgcaag cgggccccgg acccgtcgtc gtggttcagt
     7801 acggcggcca ccgcgtgggc ctcatcgtgt ccacggtcat cggcaacacc gaagtggtgg
     7861 tcaagtcgct gggaagacac tatcgggaga tcgaaggcct cctcggcgct tcgatcctcg
     7921 gaaacggccg catggcgatc atcctggacg tcggtgtcct tctggagatg cacttcaagg
     7981 ggacccagcg ggccctcccc agcttccagg cgtccgcagc ggcagccagg gcctccgatg
     8041 ccgcgcagcc ttccgtcgtg accccgcctc cccttcgcga gcccgcgccc gcgcgacccg
     8101 ctgctccgga gccgacccga gtgtctgagc cgatccgttc gcccgacccc gctcgggagc
     8161 ttgagcccgc tcgagagcct gagcccgctc gagagcctga gccctcgctg ccggtagagc
     8221 tggccgaccc gtcggcggtc gtcgagacct ccgggtcgga tagcccccaa ggtggattga
     8281 aggacctgga ggaagtgttc agctcgagcg ccctgcgggc gtcccaggcg ctgaccgatc
     8341 tcctgggaga gagcatccgg gtgagtttcc cggagacgcg attcgtccct ctgacggagg
     8401 ttgcgaaccg cctgggcggg gaggatgtgc cggtaatccg cgtgtacgtg gacgtacgag
     8461 aggggctcac gggcggacac cttctgatcg tccccgtgga ccgcgccatg ccgctttgcg
     8521 atcgcctcct gggccgaacg gccggcgcca ccaccgatat ccgggacgac gagctgtcca
     8581 gcgttgcgga gctcgggaac atcctctcgg cggcgttcat caactccata gcggaccgca
     8641 tggggatctc gatccactcc gatgtaccgg acgtgcggct ggacatgtgc ctgtccacca
     8701 tcgactccct gctggctcgg ttccaggagc cggggagcga catccttctc acggtcgcgg
     8761 aggtgacctg cggggagtcc gaacagatcg tctgccacat gatgctcttc ctggagcgcc
     8821 cttccctcgc gctgatgcgg gacgccttga ccggccgggt caccccgacg gcggagcgct
     8881 gagatcgacg cgatggaaga gcgaaccgtg gtgatccgga tagcggagat gagcgtggta
     8941 cgcagtgacg aacaggtacg atacacgctg aagacgacgc tgggttcctg tgtcggcgtc
     9001 atcctcacgg atccggtcaa ggacatccat ggcctggcgc acgtgatgct cccgcagcgg
     9061 ctggacggcg accaggccgt cggcaagtac gccgatacgg cgatcccggc gctggtcacc
     9121 gagatggagc ggtccgggag caaccgcaag gacatcgagg ctctgctggt cggcggggcc
     9181 agcatgttcc agaacgagca gacccccacc ctggcgcgca tcggggcgaa gaacgtggag
     9241 gcctcggccc gtgtcctgca gcggatgggg atccgggtgg tgttcgagga cacgggcgga
     9301 tcggcgggac ggcgggtagc cttcgacggc cgcggccgcc ccgaggtgaa gaccatcgac
     9361 gtgaggaaac tgccgtgaat ccctcggccg tcctcgccct gcccgtgctt accgacgacc
     9421 tctttgcccg gttctgcatc ctgatccagc gggagacggg gatctgcatg cgggagagca
     9481 agaagctcct ggtatccaac cgcctgagca agcgcatgcg ccagatcggg atcggcagct
     9541 tccaggaata ctacgaccgc gtgaccggat ccgacgccgc ggaacgcgtc cgattcatcg
     9601 atgccgtgac cacgaacgag acgtacttct atcggggaga gaaccacttc agcatcctcc
     9661 gccgccacat cctgcccgag cgcttcgctg gcaaggaccg ggtgaccgtc tggtgcgtgg
     9721 gctgctccac gggagaggaa gcctacacgc tggcgatcga gggcatggag gcggcgggac
     9781 ctggacgctt cctcgaggtg accgcgaccg acatcagcac ggccgtcctg gcccaggccc
     9841 aggcggcggt ctacggcggc cgcacgttgc gctaccttcc tgaggtcatc cgcggtcgct
     9901 acttcgacgc ggccggcggt gaggagtacc gggtgaagga ggaagtccgc cgccgggtgc
     9961 gattcctcca gcacaacgcc ctgcatgagg agcctccggt ccggggcgtg gatgtgatct
    10021 tctgcaggaa cgtcatgatc tacttcgacc tgccgaccca ggtccgcctg gtggatgagc
    10081 gcttcgcctc cgcgctcgcg gccgagggat acctcttcat aggacactcg gaatccctga
    10141 tcggcaagag ccgacggttc tcctacagcc gcatcgagca cgcccccgtc tatcggcttc
    10201 gaaaggaccc gtcgtgatcc gcgtgatggt cgtggatgat tcgcccctgg tgcgcaagat
    10261 cgtgacggac atcctttccg cgcagccgga catcggggtg tgcgccacgg cgccgagcgg
    10321 tgagctggcg ttgcacaagc tggagcgctc gcagccggac gtcgtgacca tggacatcag
    10381 catgcccggg atgggcgggc tcgccgccat ccggcagatc atgggcagcc gacccacacc
    10441 ggtcatcgtg ctgagcgcgt tcgcgcatcg gggagcggag cacacgctcc aggccctgga
    10501 ggccggcgcc gtagactttg tcctcaaacc gtcggtgtcc atctccggag gcgtggcgca
    10561 ggtggcgcgg gagctggtgg acaagatccg ccaggcggcg cagatcaacc tcggcctcct
    10621 ggcgcctccg ccgccggcgc ccggcgcggc ggacccgcgg ggggaagccg ccgcgccgac
    10681 acccgctctg ctgagacccg tggagatcgt cgcgatcggc gcctcgacgg gcggtcccgc
    10741 cgcgctgaag gtggtcctgc agagcctgcc ggaggacctg ccggtgggcg tggtggtggt
    10801 ccagcatatg ccggtcctct tcaccaggcc gttcgcggag cacctggatg cgatctgccg
    10861 ggtcgccgtc aaggaggccg agcacggcga tgagatcgtc gacggacggg tcctcgtcgc
    10921 tccgggggat cggcacatga ccgtgacgcg ggagggcgga cggccccggg tgctcctgaa
    10981 ccaggacgag ccggtaggcg ggcaccgccc gtcggtggac gtgctcgtgc ggtcggtagc
    11041 gcgggaatac gggtgtcgcg ccatcggcgt catcatgacc ggcatgggaa aagatggggc
    11101 ccaggggatc ggcgagctgc gggaccaggg cggatacgtg atcgcccagg actccgccac
    11161 ctccgtggtg ttcgggatga acggcgaagt ggtgcgaagg ggagccgcgc acgccgtggt
    11221 cccggtctcc gcgatcgcgg gagccatcct ctcgcggatc cactcgccgg aagcggccgg
    11281 ctgagcccgg aaggagatcg ccgtgaacat cgcagccagc tacgacctcg agatcctgca
    11341 gaaccgcggg atggagatgg tgctcaagag agccggcagc tttctggacg agcatgggga
    11401 gttctgccgc tgcgagcagt gcgtgttcga cctgctggcg ttcacgctca accacgtgac
    11461 gccgctgtac gcgacatccc tgctcggggc cctatccggg aacagcaggc tccagcagaa
    11521 gcttgagatc gagatcgaga tggccctgga ggaaggggcg aagcgcatcg ctcgtcaccc
    11581 cagccacctg gacgccgggg acacgggctc ctgaacggcg gggccggaag ggaaaaacga
    11641 tggaaggaac gaagggacgg aaggccgcgg atgcggccat ggaccagatg atcagcttct
    11701 cggtcgggac ggaacagtac ggtgtggata tccgaaaggt ccgggaggtc atcctcccgg
    11761 gagagatcac cccgctgccg cgggcgccgg acttcgtaaa gggtatgatc aatctccgcg
    11821 gagacgtgat ccccgtcatc gacctgcgcg agaagctggg acgcgccgga gggggacgcc
    11881 ccggggagga ccggatcatc gtcgtggaga tctcggagaa actgatcggg atgatcgtgg
    11941 atgcggtgag ccatgtgctc cgcgtcgcgc cgggacagct cgagcccact ccgccgtggc
    12001 tgggcggcct caccggggaa tacatcaccg gcgtggtgcg acagacgggc gggctcatcg
    12061 tggttctcaa cgtggatacc attctgacca gcggagaaaa gctggcgctg gagaacatga
    12121 cgcgctgatc ggaaatcggg caggaggtgg cctatgtcgg caagagtgaa agtctggatg
    12181 aaggtggctg gggggttcgc cattctgatc gtactgatca tcgccgttac gatgatcgcc
    12241 tacgttcaga accgctccgt ggccaaagcc gtggccagcg ccgaggcgct catggggaac
    12301 tccgcgcccc aggacatgat ggttcgcatc atcgagatcc tgcaggcgcg gatgaacgag
    12361 ggccagtacg gtctgcaggg cgggaatctg aaagacgtcg cggaccaggt aaagtccctg
    12421 aacaccatgg tggcggatct cctcaaggga ggagacctga acgcctcgca gcgcgagcgc
    12481 ctgcagaaga tggcgcagag cgcggaaacg ttccgctcgg actacatggc ctactgggtg
    12541 gagcgaggga acgaggcccg atatgcctcg gattggagcg acgccaggga ccggctggac
    12601 gccgccgcgg gtcacagcgg caacctggcg gaagccttcg accagctcca ggttaccgcc
    12661 ctcacgttcc tcaaggacaa acagccgctg gcgtgggacg cctacaccgc ggcacaggcg
    12721 gactttacct cgacggcgag ccgtgccaag agaggggacg ccgccctgat ggcggccgcg
    12781 gacgagtacg caaagaccat ggccagctac aagacgtact tcgagaagga atcggcggct
    12841 tccgtcacca tgaccaagac cgtcaacgat atcctctcaa cctcccggga cctggaacgg
    12901 gagctcacgc agatccagca gaactcctcc gctaccgcga tcctgctcat gttcatcgcc
    12961 cttgccctgg cggtgctggt gggcgtcggg atcgccttga tcatcacccg gggcatcacc
    13021 gtaccggtga cccgcgcggt ccagttcgcc cggaccatcg cgcagggaga cctgacgcag
    13081 agactggcca tcgagcagaa ggatgagatc ggcgagctgg caagctccct ggaccagatg
    13141 gccgggaagc tcggagaaat ggtgaccacc atccaggaca gcgcggagca ggtcgcggcc
    13201 agcagcgagc agatctccgc ttcctcggtc aaccttaccg agggcgccca gggccaggcc
    13261 agcaccctgg aagagaccag cgcctccgtg gaggagttgg ccgcttccat cgaccaggtg
    13321 tccagccacg ccgcctccca ggcggcggcc ttcgagcagg gggccggatc catggcccag
    13381 gtgcagaagt cgatcgagga catctccctc aacctgggga agatcgccga tatggcgcga
    13441 atgtccctgg accggtcgca ggagggcgcc caggcggtgg atcaggtcgt ggaggccata
    13501 acgctcatcg cccaaggttc ggagaggatc gcggggatcg tctcgatgat caccgacatc
    13561 gccaaccaga ccaacctgct ggccctgaac gcctcgatcg aggcggcgcg ggccggtgaa
    13621 tatggacggg gcttcgcggt agttgcggac gaggtgagca agctggccgc gcggagcgcg
    13681 acctccgcca aggagatcga gacgctgatt caggagagcg tgaagaacgt gaccgtgggc
    13741 gtggatcgcg caaaaggctc aaaggtctcc atggagcaga tcatggagac ggcgcgattc
    13801 atgtcggata tgatccacaa cgtgtccacc gccatgcagc agcaggtgtc cggcgtgcgg
    13861 gagctctcca aggcgctgga gaacatcagc gagatggcgc agagcatctc cgccgcctcc
    13921 gaggagcagt ccgcgagcgc caaggaggtt tcccgcgccg tcgaaaaggg caacgacctg
    13981 acccaacaga ccgcttcttc caccgagcag atctccgcct ccgcggagca gctctccgga
    14041 atggcctcgc agctgcagag cctcatctcc acgttcaagg tggcccgcaa cggaagtgga
    14101 aagatcgagg cgacgtcgaa ggcgccggag gcggcggcgc tgcccaaggc cgagcccgcc
    14161 cccctggagc ccgtctcggg acccgtggtg ccctcccatc catgaaggcg cgcgcgatgg
    14221 atggccgacg tgcacggatg gacggccgga acgcatgccg gccgccccga gaggaggatc
    14281 gttgatgggt agcaagacga tcggggcagc ggcgttcctg ttgctgacgc tggcctcggt
    14341 ggccatggcg gccggcaagg cagaggcaaa tggttgggta gggacgacca agccgtgctt
    14401 cacctgtcac ggaagcggct ccagcggggc caacctcctg ggggcacgcc aggagtacga
    14461 cgtctcggga cacaagacgc gcggcaactc catctatgcc aacgggggcg gttgccaggt
    14521 ctgccacacc aacgaaggct tcattcagtc ctgcgaaggg cagcagatca cggcgagctc
    14581 cttcgtggcg actccttccc agccgggctg ttttacctgc catgaccccc atgagcgagg
    14641 cgacctgagc cttcgcaccg tggctcccgt gaagctggcc tcgggagcaa cgttcgactc
    14701 cggcaacggc aacctgtgcg ccacctgcca ccatgccacc gccgcggcca gggatctggt
    14761 cgtaccgacg gcggccaaca ccattacgcc ctcctgggga gcgcaccaga gcccccaggc
    14821 ggacatcgtg gaaggcacca acctctatga gtttccgggc aagaagtaca gcagctcggc
    14881 ccacaaggac gtcatccccg acggctgcct gggctgtcat atggctttgc cgcgcggccg
    14941 ttacggcttc aactcgtcgg ttggcggaca cagcttcacc atcgtcggac aggtggaagg
    15001 ggccgtgtcg gtgaatacgg ccgggtgcat ctcctgccac aaggacctca aggcggtgcc
    15061 cggttcggat gcgttcgaca tcatcgccca ggaggactat gaccacaacg gcgtcaaaga
    15121 gccggtgcag gtcgaagtgg cagggttgct gagcgtgctg gtcaacgccc agggaaccgg
    15181 cctgttgcaa aggctggatc ccccgtttta caagtcggat gggagcttca acgaggtgac
    15241 gtcgaccgca aaacggcccg ccctggagat cgcggcgttc tacaactatc ggatgatcgc
    15301 cgacgaccga agccgcggca tccacaacgc caagtacgtc atccaggttc tctacgacac
    15361 gatccatgcc ctgaatccgc agttcgacgt ctcccgccgg ccccagtagg gggccatccg
    15421 gcgggggcgt tgtaccgctt cctcaagtcc gcacggctgt cgatcgtgct gatccaggcg
    15481 atgcgctgtc cctgctgtcc acgctgatcg cgcagggcag agaggcggcg tacgatggtc
    15541 ctcggctgcc tccaccaaaa ggagtccctg acccacgaca ttgctcttcg cgacgacggc
    15601 actcgtcctg atcggcggcc gctgcgctgg tgctcgtcgc ggtcacggtc gaccgcagca
    15661 tccagatccg cttcccggca cctaccagca cctacgaggc cctggggtcc ttctccgcca
    15721 cggtctgcgc cctgatcttc gtcctgcggc tggcgatgaa atccgatggc tcgccttgac
    15781 tagtgcgtgt ggcaggtcac gcatgccgcc ccgttccgcg gcatagggtg ctggacgttc
    15841 tgcacggtgt ggcacgtcgt gcagaccagt cccgtggtat gagcgggcac cgtgggagga
    15901 ttgggcgtcg ggaagctcga ggacaggtgc ctggcaaaga catccgtggc gttgtggcag
    15961 acgccgcagt tgccctccat ggacccgctc acgtagccgc cgccctccat gtgacagctc
    16021 aggcagtgcc ggttccacgg agacccccac gagccggtgt gagtgaacgg ctgattggtg
    16081 ctgtggcagg caatgcactg ggactggtcg tggcagacgt cgcaggactg ccggtcggtc
    16141 cgggcagcca ccccgtgcac gctttgggcc cacccgatgg tatgggtcgg gggcgcttcg
    16201 cgccggtggc aggtctggca gtcgttcccg ttggccacgg tatggcacag ggagcagtcc
    16261 tggtcgatcc gggtctgggt caggcccttg ccgtgaccgg agacccatcc gggtgtgtga
    16321 tccgcgggcc gcgtcgtgtc gaagccgtcc ccgtggcaca ccgagcagga cacggatttc
    16381 ccggagctca ggggcggctg gtggtggcac tcggtgcagc tggtgccgag ctccttggtg
    16441 tggcacaccg tgcagttcga cgcgatcaga tcccgggtga gcccggcgcc atgggagagc
    16501 ttccacgagg cagtatgcgt ctggggcgtg agcttctcca ggcctttcac gtggcaggag
    16561 tagcagtcgt tcgccccctt taccttccgg tcggtgtggc aactcacgca ggcgtccatg
    16621 gaggggagca tctcgtccgc ggtcaggacg tccttgtcga acgccgcgtg acactgcgag
    16681 cagtccacgc cggacttctg gtgctgcggg tgatcgaaca cgacgagagg ggaaaggacc
    16741 acgttcttcc gtacccgggt cttgtagtca cgctgggtgt ggcagagctc gcagtttttc
    16801 ttcggcccgt tggcgtcgat ctcgtcctga tggcacgccg cgcaggtgtc gtggtccggg
    16861 aagctcacca atcgcggatt ggccgcgttc tgttcgtggc atgcatcgca ggagaggccc
    16921 acgtccaccg tgtgatactt gtggttgaac gtgagaccgc tcaaggccct ggggaccttg
    16981 gcaaagcttc ccttgatcgc catcgacgga cccgtggacg aggatccggc gcagccccag
    17041 agaaacgagg tcaccaggac gagtgccagg aaggatgccg gcaggccccc atacaggagg
    17101 atccgagcgg ggaggctcat cccatctttc tcatgcccgt tcatgccgtg tttctcctag
    17161 tatcggaaac cggcccgcac ttcgaaacga aagtagtttc tcgcggcgtt gtcgagcccg
    17221 ctcatcgcga cggaatccac atccgggttc agcggctggg cgctcgtgat cgagctgagc
    17281 aggacgttct catacgcggc gctcagcgag aggtcgaaga agcggctgat ctgccatttc
    17341 accttcaggt agtactcctg gtcgtagaag ctgtcctcca ggaccgtctg ggtgtaatcg
    17401 gtctggtaga ggctcgcgtt gaacgacgag cccaggcgaa ggtcgaaggg gccgtccttc
    17461 ctgccgatct ccccacccac cac
//
LOCUS       DASRZS010000557         5338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3006410, whole genome shotgun sequence.
ACCESSION   DASRZS010000557 DASRZS010000000
VERSION     DASRZS010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3006410"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VFH83_13600"
     CDS             <1..835
                     /locus_tag="VFH83_13600"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6487456.1"
                     /translation="RELLADPTVDAVSIVLPDDRHRDVTLAAIRASKHILLEKPIASR
                     LTEGREIAEAARGYPKTFMVGHLLRFDARHTLAREAVAGGEIGRIIHVACRRNSTITG
                     AAMYRSHNTDTSVHLMVHDVDYINWIVGAPPARVFAKGRQLLLKEWGMQDTILATVEY
                     QDGTLAALEACWVLPAGSPSGLDDRMEIIGEKGAIYLSSSDEGVHLVTPSGTSYPDSV
                     HWPDVNGQVSGSLLDEIMSFVRCALHGERPMVGAEDALRALQVVDAIQRSLAERREVS
                     V"
     gene            complement(844..2139)
                     /locus_tag="VFH83_13605"
     CDS             complement(844..2139)
                     /locus_tag="VFH83_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET6487457.1"
                     /translation="MNIDYQHPRDQIAEIMDRIYRYGMTTTSGGNLSIRDEKGDIWIT
                     PGGTDKGVMTPQDVVRVHNGSAVETALRPSSEYPFHLAVYETRPDVRAVLHAHPPALV
                     AFSVAGTAPDTRVLPQALDACGKVDFVPYALPGSARLGALIADRLADNCDIVILENHG
                     AVAVGATLLDAFQRFETLEFCARTILNAAILGGARPLPDEMIAMRRKGWQLLQGSFQP
                     SIRGTRERELRKVLVDFVHRAYNRRLMTSTEGTFSARLSEDSFLITPYGVDRLGIGVE
                     DLAVVVSGRKEEGSEPSRAAGMHRRIYADHPEVGAIVTAQSPYVTAFIVSGVPLDSRT
                     IPESYITLRDMPVVPFEAQFRDEKAISEAITARNPVIILGNDAVMATGRTVLEAYDRL
                     EVAEATANALIAARRLGGFRPIDDAAVEEIEVAFRIPKR"
     gene            complement(2136..3017)
                     /locus_tag="VFH83_13610"
     CDS             complement(2136..3017)
                     /locus_tag="VFH83_13610"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487458.1"
                     /translation="MRQSVGDQRRKTAAGQVEWAGRSLTPRRKQVIVAVDHGLPLDRV
                     EGLERPLGVMRSLAANSEVDGTIASLGLLKTCDRLGIDLSRMARILTVDYVGFQTSGG
                     TTILSTREIILEPEAAAEVQAHAFKMFLNLYDDSDLLMGNIKDAERFVSAAVRTGVPT
                     LLEVVFYGNRGFADPRTQAAVFTRGCRLAMELGADALKVPMIEDAAAAAEAIDSVGVP
                     TFVLGGARWESRQALQAELSRIAALPVCGVMFGRNVWQTEDIAQTVSEIAGVLRPQGH
                     GSQRKGAERKGAKRAAG"
     gene            complement(3061..4053)
                     /locus_tag="VFH83_13615"
     CDS             complement(3061..4053)
                     /locus_tag="VFH83_13615"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:NF014753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HET6487459.1"
                     /translation="MKKIINQASAIVEETLDGFAELHSERVARVPGTHMIERRRQKQP
                     GRVRFMMGNGAGHEPAVIGWVGPGMFDLNVAGEIFTAPSAQDILAGIERLAASGPVLL
                     AVQNHAGDVLNANKAMELALRKGLSVKSVLFYDDIASAPKGHEQERRGMAGMLFYTKI
                     VGAAAERGYDVDRLVPLFERCRDQTRTYAVAITNCTNPITGLGMFDHLGPDEIELGLG
                     VHGDSSGNVVKLPSASGLARLMLDAIHEDFPLKRGDDLLVFVNGAGGTTMMELCIYYR
                     ELKRRLTELGIRVRGVKAGNYLTTQELSGVSLSLCRVDREMMDLWSDPCDVAAF"
     gene            complement(4050..4697)
                     /gene="dhaL"
                     /locus_tag="VFH83_13620"
     CDS             complement(4050..4697)
                     /gene="dhaL"
                     /locus_tag="VFH83_13620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HET6487460.1"
                     /translation="MRIQEFAAVFRTAAQGMAQYRDELCRLDSGIGDGDHGVTVSRGF
                     LAAAAASEEREGFSALFAAIGDAMESATGGAIGPLYAAFFAAVAEAAPEGKDSTDVAL
                     LTALAAGAAGVARAGACQEGEKTVLDAMAPWARALRAAAEAGEDVPSAARKGAEAADA
                     GARATGGMRATKGRARYRGERSIGPIDPGARSFALFAALLASALGGRSAEGGGSP"
     gene            4843..>5338
                     /locus_tag="VFH83_13625"
     CDS             4843..>5338
                     /locus_tag="VFH83_13625"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6487461.1"
                     /translation="MRNGFRLGRIAVMVGVLLTVLAVGVFAGGTSESGSAKPVTITVT
                     SWRTDDINQMATINAAFMKKYPNITVKFDPIKNTEYSAQLQTALETKSDRYDVIGVFP
                     FSWIQTYDKNGYVQPLEGLVKNLSNLPKAVLDKYSASGHVEAVPVAAVAHGIYYNKDI
                     FAKYN"
BASE COUNT          913 a         1829 c         1687 g          909 t
ORIGIN      
        1 ccgcgagctc ctggcggatc cgactgtgga tgcggtcagc atcgtcctgc ccgatgaccg
       61 gcaccgggat gtgacgctgg ccgccatccg agcctccaag catatcctct tggagaagcc
      121 catcgcatcc cggctgaccg agggacggga gatcgcggag gcggcgcgcg gctatcccaa
      181 gaccttcatg gtcggacacc tgcttcgctt cgacgcccgc cacacccttg cgcgggaagc
      241 ggtggccggc ggggagatcg ggcgaatcat ccacgttgcc tgccgacgca acagcaccat
      301 caccggtgcc gcaatgtacc ggagccacaa cacggacacc tcggtccacc tcatggtcca
      361 tgacgtcgac tacatcaact ggatcgtcgg agccccgccg gcgagggtct tcgccaaggg
      421 ccggcagctc ctcctcaagg agtggggaat gcaggacacc atcctggcaa cggtcgaata
      481 ccaggacggc acgttggccg ccctggaggc ctgctgggtc ctgccggccg gttctccgag
      541 cgggctggac gaccggatgg agatcatcgg cgagaagggg gcgatctacc tctcctcgag
      601 cgatgaaggg gtgcacctcg tgacgccttc gggaacctct tacccggact cggtccactg
      661 gcccgacgtg aacggacagg tctccggctc cctcctggat gagatcatga gcttcgtgcg
      721 gtgcgcgctt cacggggaac ggccaatggt gggcgcggag gacgccctgc gggcgctcca
      781 ggtcgtagac gccatccagc ggtcgttggc ggagcggcgc gaagtgtcgg tctgagcctg
      841 ccgctatcgc ttcggaatcc tgaacgccac ctcgatttcc tccaccgccg cgtcatcgat
      901 gggacggaag ccaccgagcc tcctggcggc aatgagcgcg ttggccgtag cctccgccac
      961 ctcaagccgg tcgtaggctt ccaggacggt gcgcccggtc gccatcaccg cgtcgtttcc
     1021 caggatgatg acgggattgc gcgcggtaat ggcttcgctg atcgccttct cgtcccggaa
     1081 ctgcgcctcg aagggcacca cgggcatgtc ccgcagcgtg atgtaggatt cggggatggt
     1141 gcgcgaatcc aaaggcaccc cggacacgat gaacgcggtg acgtacgggg actgcgccgt
     1201 cacgattgcg ccgacctcgg gatggtccgc gtagatgcgc cgatgcatcc ccgccgctcg
     1261 ggatggctcg cttccctcct cctttcgccc ggacacaacg acggccaggt cctcgacgcc
     1321 aataccaagg cgatccacac cgtagggcgt gatgaggaag gagtcctcgc tgaggcgcgc
     1381 ggagaacgtg ccctccgtgc tcgtcatgag gcgtcggttg taggcacgat gaacgaaatc
     1441 caccaggacc ttgcgaagct cgcgctcccg ggtgccccgg atggatggtt ggaagctgcc
     1501 ctgaaggagc tgccagccct ttcggcgcat ggcgatcatc tcgtccggaa gcggcctggc
     1561 gccgccgaga atagcggcat tcagaatggt gcgagcgcag aactcgagag tctcgaagcg
     1621 ctggaaagcg tccagaagag tcgcgcctac ggcaaccgct ccgtgattct ccaggatcac
     1681 gatgtcgcag ttatcggcaa gcctgtccgc tatgagagct cccagccggg cgctgccggg
     1741 aagcgcgtag gggacgaaat ccaccttgcc gcaggcatcc agggcctgcg gcagcacacg
     1801 cgtgtccggt gcggtcccgg ccacgctgaa ggcaaccaga gccggcggat gcgcgtggag
     1861 gaccgcccgt acgtcagggc gggtctcata gacggcgaga tggaacgggt actcgctgga
     1921 aggtctgagc gccgtctcca cggcggaacc attgtgcacc cgaacgacat cctggggcgt
     1981 catgacgccc ttgtccgtgc cgcccggcgt gatccagatg tcgcccttct cgtcgcggat
     2041 ggagaggttg ccccccgatg tcgtagtcat gccatagcga tagatccggt ccatgatctc
     2101 cgcgatctgg tcacggggat gctggtagtc gatgttcatc cggcggctct tttcgctcct
     2161 ttgcgttccg ctccctttcg ctgtgagccg tgcccttggg gacggaggac acccgcgatc
     2221 tcggaaacgg tctgggcgat gtcttccgtc tgccagacgt tccggccgaa catcaccccg
     2281 cagaccggga gtgccgcaat ccggctcagc tcggcctgga gagcctggcg gctctcccaa
     2341 cgcgccccgc ccaggacgaa cgtggggact cccacggagt cgatcgcttc cgccgcggcc
     2401 gcggcgtcct cgatcatcgg gaccttgagc gcatcagcgc cgagctccat cgcaagccgg
     2461 cagcccctcg tgaagaccgc cgcctgggtc cgagggtcag cgaagcctcg gttcccgtag
     2521 aacaccactt ccagcagcgt agggacgccg gtcctcacgg ccgcgctcac gaagcgctcc
     2581 gcgtccttga tgttgcccat cagaaggtcc gaatcgtcat acaggttcaa gaacatcttg
     2641 aaggcatgcg cctggacctc cgcggccgcc tccggctcta ggatgatctc acgcgtggag
     2701 aggatggtcg tgccgccgga cgtttgaaag cccacgtagt ccacggtcag gatgcgcgcc
     2761 atgcgggaca ggtcgatccc cagcctgtcg caggtcttga gcagccccag cgaggcgatc
     2821 gtcccatcga cctcggagtt cgccgccagg gagcgcatga ccccgagcgg ccgttccagc
     2881 ccttccacac ggtccagcgg caggccgtga tcgacggcca cgatgacctg cttgcggcga
     2941 ggcgtcaggc tccgccccgc ccactcgacc tgcccggccg cggtcttcct gcgttgatct
     3001 cccacggact gcctcatctc acgactcctt cagaccggtg tggtttggtt gaccgcttcc
     3061 tcagaaagcg gccacgtcgc acggatcgct ccacaggtcc atcatctcgc gatcgaccct
     3121 gcagagagaa agcgataccc cggagagctc ctgggttgtc aggtagttgc cggccttcac
     3181 gccgcggacc cggatgccca gctcggtcag tctgcgcttc agctctcggt agtaaatgca
     3241 cagctccatc atcgtggtcc ccccggcccc gttcacgaag acgagaaggt catctccgcg
     3301 cttcaggggg aagtcttcgt gtatggcgtc gagcatcagc cgggcgaggc ccgaggcgct
     3361 cggaagcttg acgacgttcc ccgaggaatc accgtgaacg cccagaccca gctcgatctc
     3421 gtcgggtccg agatggtcga acattcccaa tccggtgatc ggattggtgc agttagtgat
     3481 ggctacggcg tacgtccggg tctgatcgcg gcatcgctcg aacaggggga ccagccggtc
     3541 aacgtcgtat ccccgttccg cggcggctcc cacgatcttg gtgtagaaaa gcatcccggc
     3601 catgccccga cgctcctgct cgtgcccctt gggagctgag gcaatgtcgt catagaagag
     3661 gacggacttc acggacaaac ccttgcgcag cgccagctcc atggccttgt tggcgttgag
     3721 tacgtcaccg gcgtgattct ggaccgccag cagcaccgga ccgcttgccg ccaggcgctc
     3781 gatgccggcc aggatgtcct gggccgacgg tgccgtgaag atctccccgg cgacgttcag
     3841 gtcgaacatc ccgggcccta cccacccgat cacggcgggc tcgtgcccgg ccccgtttcc
     3901 catcatgaac cggacccttc cgggctgctt ctgcctgcga cgttcgatca tatgcgtgcc
     3961 cggtaccctg gccacgcgct cggagtgaag ctcggcgaac ccgtccaggg tctcctcgac
     4021 gatcgctgag gcctgattga tgatcttctt cacggggagc cccctccttc cgccgagcgc
     4081 ccgccgaggg cggacgccag cagcgccgca aaaagggcga acgaccgggc gcctggatcg
     4141 atcgggccga tcgagcgctc gcctcggtag cgcgcgcggc ccttggttgc ccgcattcct
     4201 ccggttgccc gggcgcccgc atcggcagcc tcggctccct ttcgggcggc gctggggacg
     4261 tcctcaccgg cctccgcggc tgccctcagg gcccgcgccc acggcgccat cgcgtccagc
     4321 accgtcttct cgccttcctg gcaggcgccg gctcgggcta ctcccgccgc cccggctgcg
     4381 agcgcggtga ggagggcgac gtcggttgag tctttcccct ccggcgcggc ttctgccacc
     4441 gcggcaaaga aggccgcgta cagcgggcca atggcgcctc ccgtggcgga ctccatggcg
     4501 tccccgatgg cggcaaacag ggcggagaag ccttcccgct cctcggaggc ggccgccgcg
     4561 gcaaggaacc cccgcgacac cgtgaccccg tggtctccgt cgccgatacc ggagtcgagc
     4621 ctgcacagct cgtcacggta ctgcgccatg ccttgagccg ctgttcggaa cacagcggcg
     4681 aactcctgga ttctcatctc caccgcccct gccgcgcttt ggatcgcgcg ctcagcgctg
     4741 ccgacccgat ccttctaccc ttacgcccgg tatccgtcaa gcgcgccatg tccttgaccg
     4801 ggctcgggtg ctgtgagacc atcagccaag gaggaaactc agatgagaaa cggcttcaga
     4861 ctcggcagaa tcgccgtcat ggtcggggtc ctgctgaccg tgcttgcggt gggcgtgttc
     4921 gccggaggca cctcggagag cggctcggcg aagcccgtga ccatcacggt gacaagctgg
     4981 cgtacggacg acatcaacca gatggccacg atcaacgcgg cgttcatgaa gaagtacccg
     5041 aacatcacgg tgaagttcga tccgatcaag aacaccgaat actccgccca gctccagacc
     5101 gcgctggaga ccaagtcgga tcgctacgac gtgatcggcg tattcccctt cagctggatc
     5161 cagacgtacg acaagaacgg ctacgtgcag ccgctggagg gcctggtcaa gaacctctcc
     5221 aatctgccca aggcggttct ggacaagtac tccgcaagcg gtcacgtaga ggccgtcccg
     5281 gtggccgccg tggcgcacgg gatctactac aacaaggaca tcttcgccaa atacaacc
//
LOCUS       DASRZS010000558         8199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5314711, whole genome shotgun sequence.
ACCESSION   DASRZS010000558 DASRZS010000000
VERSION     DASRZS010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8199
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5314711"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VFH83_13630"
     CDS             <1..715
                     /locus_tag="VFH83_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HET6487462.1"
                     /translation="GPPGVEGQTPPAYKDLSNLVGKYDKTSQTTNNEINKKVTTAERQ
                     PWDIRGISTGVAIDGVWKERYDKGGRVVLGPDGSIQRDYSAVSDDDLAKAKALIQAAI
                     GYNRDRGDTVEVQTIPFDRTTQFEKEDSVFRRALQVRRIIYGALIGLGALLLLALAYR
                     LIAKELERRRRLREEELARQHQAMREAALRTAEEQGVEVELSVEDRARLEMQENAANM
                     AREHPEDVAQLIRTWLVEE"
     gene            719..1843
                     /gene="fliG"
                     /locus_tag="VFH83_13635"
     CDS             719..1843
                     /gene="fliG"
                     /locus_tag="VFH83_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HET6487463.1"
                     /translation="MAKQPAKPGARPLAPAGGAKTADDKERTVGGPAPAKGGKASRKA
                     LTGRQKAAVFLVTLGSEIASEIFKHLREDEIEQLTFEIARLESIEPQERDQVLMEFHE
                     LMMAQEFISTGGIDYARELLEKSLGTQKAVDIINRLTSSLQVRPFDFIRRTDPAHLLN
                     FIQQEHPQTIALILAYLEPQKASVILGSLPHEIQSDVAKRIATMDRTSPEVLREVERV
                     LEKKLSTLSSEDYTAAGGVESIVEILNMVDRSTEKTIVESLEEDDPELAEEIKKRMFV
                     FEDIVLLDDRAIQKVMREVDTAELAKALKSVDSEVQDKIYRNMSKRASTLLKEDMDYM
                     GPIRLKDVEEAQQRIVAIIRKLEEQGEIVVARAGEEELVV"
     gene            1879..2808
                     /gene="fliH"
                     /locus_tag="VFH83_13640"
     CDS             1879..2808
                     /gene="fliH"
                     /locus_tag="VFH83_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HET6487464.1"
                     /translation="MAKNVFKPQEIMYLSRKIFIEPPRISSSEEPAQPVEVAPAQTEE
                     GEFTGPTVEDLRREAEAFRKEWEIEKQRLLSQAQEQADRIKKEAEQVAFDEVKRKNNQ
                     AQKIRQEAEDQAQKIVADARQKAAALEEEVKGRVAQVEKEATERGQAEGHAKGYEEGR
                     AEVARLVESLHAIIAKAIEKRNEIIEEAETQIINLVLLIVKKVIKVISENQKNVVINN
                     VVQALRKLKSRGDVVIRVNLADLELTSEHVKDFMKMVENVKSITVLEDSSVDRGGCII
                     ETDFGQIDARISSQLHEIEERILELMPIRSKGE"
     gene            2820..4181
                     /locus_tag="VFH83_13645"
     CDS             2820..4181
                     /locus_tag="VFH83_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HET6487465.1"
                     /translation="MGVFDKYSVVVQDLDPIKFTGVVERVQGLLLESHGPAAQVGELC
                     QILLPHGRGVVWAETVGLRGKTVQLMPYDEIEGIEIGSLVIGMGETLRIPVSPKLLGR
                     TLDSMGKPIDGKGDIGSEVWYPAVNAPPDVLRRRRIREVLPTGVRSIDGLLTVGKGQR
                     MGIFAGSGIGKSTLLGMIARNTTADVNVIALIGERGREVREFIENDLGPEGLARSVIV
                     VSSSNKPPLARIRGAYVATAVAEYFRDQGKDVMLLFDSVTRFARAQREIGLAIGEPPA
                     NRGFTPSVFSLLPKLLERSGTSDRGSITGFYTILVEGDDIDDPISDNVRGILDGHIIL
                     TRRLASRNHYPAVDVLQSISRLALQVTGARTKEAMGAIRRLMATYANAEDLISVGAYV
                     KGSNAEIDEAIEKMPAIERFLVQGIAEKAPLAETLRLASEITGVPIPSEELPADAAVP
                     VSA"
     gene            4156..4599
                     /gene="fliJ"
                     /locus_tag="VFH83_13650"
     CDS             4156..4599
                     /gene="fliJ"
                     /locus_tag="VFH83_13650"
                     /inference="COORDINATES: protein motif:HMM:NF014144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HET6487466.1"
                     /translation="MRRFQFRLERFLDLKRWKERECEIALARVLGECLLLERRIAEIG
                     TLIGDSLVPAGVRGSAIDVIALAQREHYVQRLAVERQRARTTLEQKRKELQAARDKYL
                     QASKERRVLDKLKERRSAEYYDHARDEEFKGIDEMNVASATRQGW"
     gene            4624..5253
                     /locus_tag="VFH83_13655"
     CDS             4624..5253
                     /locus_tag="VFH83_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487467.1"
                     /translation="MAEFSDVRAGPRIVLLFLLLIVLSLGGIIWFDYLGLIDAHSWLG
                     PVYRLVGAQRASPAGSADDPNLLAMERMAKEAQALDLRQQELDNRDAALSQREKQLDQ
                     LGQDLADKEAALANQQKAFNDQQKAFENRSVNLQQNARYLTSMAPQSAVNILMKMDDQ
                     DIIDNFRTVEAQSATAGTDSPVPYWMSLMPADRAAALQRKMTRAGGATP"
     gene            5348..6496
                     /locus_tag="VFH83_13660"
     CDS             5348..6496
                     /locus_tag="VFH83_13660"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HET6487468.1"
                     /translation="MRGKADPGKADPFLAIFLAKTASAEAAAAKTRSLRLPPGKLPGA
                     KPGAPDLLDPKLAVGKGKPSGSVHSDAGTGKASRKLSDAVAALKPSTAKSELVLSPEG
                     PHGKVEEKRSDEKKKTPARAAEATPSAAPIAVGHAVLGLAPAEARKASADAAPAPAQA
                     LQGAARQSSSVAAEPKVVVVDLRKKQQAASAEAPGDARASLSRLAASDKDASVPVLQR
                     VGPDGGQPSAGPVTPRLDAPPAAHPDSMQRLRDMASSEVVKAANLVVRDGGGEIRLVL
                     KPESLGSVRIKMSLVDNSIHGRIIVDTPAAKQIIDGSLDSLIRAFTADGFQNASVQVS
                     VSGQGTDQGRGAQETPPVVRRRSPEGFEGSVPGIQDASLGDLLVNLFV"
     gene            6536..7048
                     /gene="flgD"
                     /locus_tag="VFH83_13665"
     CDS             6536..7048
                     /gene="flgD"
                     /locus_tag="VFH83_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HET6487469.1"
                     /translation="MELSLSLSPQEQLDVSREVDRFNKTLNGGSGSKSANVLDKNDFL
                     KILITQLSHQDPTQPMDDKEFIAQMAQFSSLEQITNMSTGLSKVADLLAKSQAVGLLG
                     STVDVSDGSGTVSGIVDAVTGGDYPQILVGGQYYDASQVQKILRAAKGAEALTGSADA
                     LKGSADALKE"
     gene            7055..>8199
                     /locus_tag="VFH83_13670"
     CDS             7055..>8199
                     /locus_tag="VFH83_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HET6487470.1"
                     /translation="MMRSLWAGVAGLQNHQTRMDVLGNNIANVNTNGFKKGRVTFQDM
                     LSQTLQGAARPTDTVGGVNPQQVGLGMTVASIDTIHTQGALQSTGVVTDMAIQGNGFF
                     MVNDGDQRFYTRNGAFGLDQNGLLVNPANGMRVQGWTAQTIGGQTFVNNAAEVGDLTI
                     PVGSKDPAKATTAVFFASNLDKRTPEIPAGAGPQTVQAGTWQTTFDVFDSFGRIHKLE
                     VDFTRVPGVANRWQATVNVDPAAQTPTNARVQIGNGPGATNTFLLDFDNFGALAGVRD
                     AQGNAITAGALQAQVSYDVPDATLPPGAATLREGFSLNLGQVGAYRDAITQFAETSST
                     KAVRQDGYGMGYLETFKIDQSGVITGVYTNGSNRILGQIGLASFTNPG"
BASE COUNT         1596 a         2587 c         2857 g         1159 t
ORIGIN      
        1 ggggcccccg ggggtggagg gacagacccc gccggcgtac aaggaccttt ccaacctggt
       61 gggcaagtac gacaagacct cccagaccac caacaacgag atcaacaaga aggtcactac
      121 ggcggagcgc cagccgtggg acatccgcgg gatcagcacc ggcgttgcca tcgacggcgt
      181 atggaaagag cggtatgata agggggggag ggtggtgctg ggccctgacg gcagcatcca
      241 gcgggactac tccgcggtct ccgacgacga cctcgccaag gccaaggccc tcatccaggc
      301 cgccatcggg tacaaccgcg accgcgggga caccgtggaa gtccagacca tacccttcga
      361 ccgcaccacg cagttcgaga aggaagacag cgtcttccgg cgggcgttgc aggtgcggcg
      421 gatcatctac ggcgcgctca tcggcctggg cgcccttctc ctcctggccc tggcctaccg
      481 cctgatcgcc aaggagctgg aacggcgtcg gcgcctgcgg gaagaggagc tggctcgcca
      541 gcaccaggcc atgcgcgagg cggccctccg cacggcggag gagcagggag tggaggtcga
      601 gctgtcggtg gaggaccggg cgcgcctcga gatgcaggag aacgcggcca acatggcgcg
      661 cgagcatccc gaggacgtgg cccagctcat ccgcacctgg ctcgtggagg agtagcgtat
      721 ggcaaagcag ccggcaaagc ccggggcgcg gcccctggcg cccgcgggcg gcgccaagac
      781 ggcggacgac aaggagcgca cggtcggcgg accggctccg gccaagggcg gcaaggcaag
      841 ccgcaaggcg ctgaccgggc ggcagaaggc cgcggtgttc ctggtcacgc tgggctcgga
      901 gatcgcctcc gagatcttca agcacctgcg ggaagacgaa atcgagcagc tcaccttcga
      961 gatcgcgcgc ctggagagca tcgagccgca ggaacgcgac caggtgctca tggagttcca
     1021 cgagctgatg atggcccagg agttcatctc cacgggcggc atcgactacg cgcgcgagct
     1081 gctggagaag tccctcggca cgcagaaggc cgtggacatc atcaaccgcc tcacgagctc
     1141 cctgcaggtg aggccgttcg acttcatccg gcgcacggat cccgcgcacc tcctgaactt
     1201 catccagcag gagcacccgc agaccatcgc gctgatcctg gcctacctgg agcctcagaa
     1261 ggcctcggtc atcctgggca gcctcccgca cgagatccag tccgacgtgg ccaagcgcat
     1321 cgccaccatg gaccgcacct cacccgaggt ccttcgggag gtggagcggg tgctggagaa
     1381 gaagctctcc accctgtcgt cggaggacta cacggccgcc ggcggagtcg agagcatcgt
     1441 cgagatcctc aacatggtgg accgctccac ggagaagacc atcgtggaga gcctggaaga
     1501 ggacgacccc gagctggccg aagagatcaa gaagcgcatg ttcgtgttcg aggacatcgt
     1561 gctcctggac gaccgcgcca tccagaaggt catgcgcgag gtggacacgg cggagcttgc
     1621 caaggccctg aagagcgtag actccgaggt ccaggacaag atctaccgca acatgtccaa
     1681 gcgggcgtcc acgctcctca aggaagacat ggactacatg ggccccatcc gactcaagga
     1741 cgtggaagag gcgcagcagc gcatcgtggc catcatccgc aagctggagg agcaggggga
     1801 gatcgtcgtg gcgcgcgctg gagaggaaga gctggtggta tagccggcgc cggggcgccc
     1861 gctgcttagg agagttccgt ggcgaagaac gtattcaagc cccaggagat catgtacctc
     1921 tcccggaaga tcttcatcga gcctcccagg atctcctcct cggaggagcc ggcccagccg
     1981 gtcgaggtgg cgcccgcgca gaccgaagag ggagagttca ccggccctac cgtggaggac
     2041 ctgcgccggg aggcggaggc gttccgcaag gaatgggaga tcgagaagca gcggctcctg
     2101 tcccaggcgc aggaacaggc ggaccggatc aagaaggagg ccgagcaggt cgccttcgac
     2161 gaggtgaaac ggaagaacaa ccaggcccag aagatccgcc aggaggccga ggaccaggcc
     2221 cagaagatcg tggccgatgc ccggcagaag gccgccgcgc tggaagagga ggtcaagggc
     2281 cgcgtggcgc aggtggagaa ggaagccacc gagcgcgggc aggccgaggg ccacgcgaag
     2341 ggctacgagg agggccgtgc cgaggtggcg cggctcgtgg agagcctgca cgccatcatc
     2401 gcgaaggcca tcgagaagcg caacgagatc atcgaggagg cggagaccca gatcatcaac
     2461 ctggtgctcc tgatcgtgaa gaaggtcatc aaggtcatct cggagaacca gaagaacgtg
     2521 gtgatcaaca acgtggtcca ggcgcttcgc aagctgaaga gccggggcga cgtggtgatc
     2581 cgcgtgaacc tggcggacct cgagctgacc tcggagcacg tcaaggactt catgaagatg
     2641 gtggagaacg tcaagtcgat caccgtcctg gaggattcct ccgtggaccg gggcggctgc
     2701 atcatcgaga cggacttcgg acagatcgac gcgcgcatct cctcgcagct ccatgagatc
     2761 gaggagcgga tcctggagct catgcccatc cgctccaagg gcgagtagga gagccgccca
     2821 tgggtgtgtt cgacaagtac tccgtggtgg tgcaggacct ggatcccatc aagttcaccg
     2881 gcgtggtgga acgggtccag ggactgctgc tggagagcca cggcccggcg gcccaggtgg
     2941 gggagttgtg ccagatcctt ctgccgcacg gcagaggcgt tgtctgggcg gaaacggtgg
     3001 gtctccgcgg caagaccgtg cagctcatgc cgtacgacga gatcgagggg atcgagatcg
     3061 gctccctggt catcgggatg ggggagacgc tgcggatccc ggtctccccg aagctcctgg
     3121 ggcggacgct ggactccatg gggaagccta tcgacggcaa gggagacatc ggatccgagg
     3181 tgtggtatcc cgccgtgaac gcgcccccgg acgtgctacg gcgacgaagg atccgcgagg
     3241 tcctgcccac gggggtccgc tccatcgacg gcctcctgac ggtgggcaag ggccagcgca
     3301 tggggatctt cgccggaagc ggcatcggca aatcgacgct cctggggatg atcgcccgca
     3361 acaccacggc ggacgtcaac gtcatcgcgc tgatcggaga gcggggccgg gaggtccggg
     3421 agttcatcga gaacgatctg ggcccggagg gtcttgcccg ttcggtgatc gtggtctcct
     3481 cttccaacaa gcccccgctc gcgaggatcc gcggcgccta tgtggccacc gccgtggcgg
     3541 agtacttccg ggaccagggc aaagacgtga tgctcctgtt cgactccgtg acgcggttcg
     3601 cccgcgccca gcgggagatc gggcttgcca tcggggagcc gccggccaac cgtgggttca
     3661 ccccttcggt cttctccctg cttcccaagc tgctggagcg cagcggcacg tcggatcggg
     3721 gaagcatcac cgggttctac accatcctgg tggaggggga cgacatcgat gatcccatct
     3781 ccgacaacgt tcgtggcatc ctggatgggc acatcatcct gacccggagg cttgccagcc
     3841 gcaaccacta tccggcggtg gacgtgctgc agtccatctc ccgattggcg ctccaggtga
     3901 cgggggctcg gaccaaggaa gccatgggcg ccatccggcg gctgatggca acgtacgcca
     3961 acgccgagga cctgatcagc gtgggcgcgt acgtaaaggg cagcaacgcg gagatcgacg
     4021 aggccatcga gaagatgcct gccatcgagc ggttcctggt ccagggcatc gcggagaagg
     4081 cgccgctggc ggagacgctg cggctggcct cggagatcac gggcgtgccg atcccgtcgg
     4141 aggagcttcc cgccgatgcg gcggttccag tttcggcttg agcgattcct cgacctgaag
     4201 cgctggaagg agcgggagtg tgagatagcc ctggcccggg tgctggggga atgcctgctc
     4261 ctggaacggc ggatcgcgga gatcgggacc ctgatcggag acagcctggt accggcgggc
     4321 gtccggggga gtgccatcga cgtcatcgcg cttgcccagc gggaacacta cgttcagcgg
     4381 ctggccgtgg agcgccagcg cgcgcggacc accctggagc agaagcgcaa ggagctccag
     4441 gcggctcggg acaagtacct ccaggcgtcg aaagagcgcc gcgtgctgga caagctcaag
     4501 gagcggcgtt ccgcggagta ctacgaccac gcacgggacg aggagttcaa gggcatcgac
     4561 gagatgaacg tggcgagcgc cacgcggcag gggtggtaga cctcgaccca agggaggggt
     4621 gccgtggcag agttcagtga tgtgcgggca ggaccgcgga tcgtgctctt gtttcttctc
     4681 ctgatcgtcc tcagcctcgg ggggatcatc tggttcgact acctggggtt gatcgatgcg
     4741 cattcgtggc tgggcccggt gtaccggctg gtcggcgccc agcgggccag cccggcgggg
     4801 agcgccgatg accccaacct cctggcgatg gagcgtatgg ccaaggaggc tcaggcgctg
     4861 gacctgcgcc agcaggagct ggacaaccgc gacgccgcgc tgagccagag agagaagcag
     4921 ctcgaccagc tcggccagga cctggcggac aaggaagccg ccctggcgaa tcagcaaaaa
     4981 gcgtttaatg accagcagaa agcattcgag aatagaagtg tgaacctgca gcagaatgcc
     5041 cgttacctga cgagcatggc accgcagagc gccgtgaaca tattgatgaa gatggacgac
     5101 caggacatca tcgacaactt ccggaccgtg gaggcccagt ccgccaccgc gggaaccgac
     5161 tccccggtac cgtactggat gagcctgatg cccgcggacc gcgccgctgc cctgcagcgc
     5221 aagatgaccc gggccggcgg agccacccct tgagcatccc cgccgccatg accgcggagc
     5281 ctcacggaag cgcgcagaag gcctcccccc acgcggcgga gcaggcggct gccggcaaga
     5341 cctcccggtg cgcggcaagg cggatccggg caaagcggat ccgttcctgg caatcttctt
     5401 ggccaagacg gcgtccgcgg aggcggctgc cgccaagaca cgttccctcc gtttgccgcc
     5461 cggcaagctg ccgggcgcga agcctggcgc gcccgatctt ctggacccca agctcgcggt
     5521 cggcaaaggg aagccgtccg ggtcggtgca ctcggacgct ggaaccggca aagcttcccg
     5581 caagctctct gacgcggtcg cggccctgaa gccgtccacc gcgaagagcg agctggttct
     5641 ctcccccgag gggccgcacg gaaaagtcga ggagaagagg tccgacgaga agaagaagac
     5701 gccggccagg gccgcggaag cgactccgtc ggcggctccc atcgccgtcg gtcacgcggt
     5761 gctgggtctg gcgcccgccg aggcacggaa agcgtccgcg gatgccgccc cggctcccgc
     5821 gcaggctctc cagggcgcag cgcggcaatc ttcgagcgtc gcggcggaac cgaaggttgt
     5881 ggtggtggat ctgcgaaaga agcagcaggc tgcgtcggcg gaggcgccgg gtgacgcccg
     5941 ggcctctctg agccggctcg ccgcgtcgga caaggacgcc tccgtacccg ttttgcagcg
     6001 cgtgggcccg gacggcggcc agccttccgc gggaccggtg acgcctcgtt tggacgcacc
     6061 gccggcggcg catccggact ccatgcagcg gttgcgggac atggcgagct ccgaggtcgt
     6121 gaaagccgcc aacctggtgg tgcgggacgg cggtggcgag atacgcctgg tcctgaagcc
     6181 ggagtccctg ggaagcgtcc ggatcaagat gagcctggtc gacaacagca tccatggcag
     6241 gatcatcgtc gacaccccgg cggcaaagca gatcatcgac gggagcctgg actccctgat
     6301 ccgcgcgttc acggcggatg ggttccagaa cgcgtcggtg caggtgtccg tgagcggtca
     6361 agggacggat caggggcggg gcgcccagga gaccccgccg gtggttcgtc gccggtcccc
     6421 ggagggcttc gagggcagcg tgccaggaat ccaggacgct tccctgggag atctcctggt
     6481 gaacctgttc gtgtagcgcg gagcccctgg ctcgcgcgca agtggaggat agcagatgga
     6541 gctttcgctt tccctttctc cccaagagca gctcgacgtg tcgcgagagg tggaccggtt
     6601 caacaagacc ttgaacgggg gaagcgggtc gaagtccgcc aacgtcctgg acaagaacga
     6661 tttcctcaag atcctcatca cgcagctcag tcaccaggat cccacccagc ccatggatga
     6721 caaggagttc atcgcccaga tggcccagtt ctccagcctg gagcagatca cgaacatgag
     6781 caccggtctg agcaaggtgg cggatctgct ggccaagagc caggccgttg gtctcctggg
     6841 cagcacggtg gacgtctccg acggaagcgg gaccgtgtcc ggtatcgtgg acgcggtgac
     6901 cggcggagat tacccccaga tcctggtcgg cggacagtac tatgatgcat cccaagtgca
     6961 gaagatcctg cgcgcagcga agggcgctga ggcgctgacg ggaagcgctg atgcgctcaa
     7021 gggaagcgct gacgcgctca aggagtaacg gcgtatgatg cgatccctct gggccggcgt
     7081 ggccggcctg cagaaccacc agacgcgcat ggacgtcctg ggcaacaaca tcgccaacgt
     7141 gaacaccaac gggttcaaga agggacgcgt gacgttccag gacatgctgt ctcagacgct
     7201 tcagggcgcg gcccgaccaa cggacacggt cggcggcgtg aacccgcaac aggtggggct
     7261 cgggatgacg gtggcctcca tcgatacgat ccatacccag ggggcgctcc agtccaccgg
     7321 cgtggtgaca gacatggcca tccagggcaa cggattcttc atggtgaacg acggcgacca
     7381 gcgcttctac acgcgcaacg gcgcgttcgg cctcgaccag aacggtctgc tggtgaaccc
     7441 tgccaacggc atgcgcgtcc agggctggac cgcgcagacc attggcggac agaccttcgt
     7501 gaacaacgcc gccgaggtcg gggacctcac cattcccgta ggcagcaagg atccggccaa
     7561 ggccacgacg gcggtgttct ttgccagcaa cctggacaaa cggactccgg agatcccggc
     7621 gggtgccgga cctcagaccg tccaggccgg tacctggcag accaccttcg acgtcttcga
     7681 ctcgttcggc cggatccaca agctggaggt ggacttcacg cgggtgcccg gcgtggcaaa
     7741 ccgctggcag gccacggtca acgtcgaccc ggcggcccag acgcccacca acgcaagggt
     7801 gcagatcggc aacggcccgg gcgccacgaa cacgttcctg ctggacttcg acaacttcgg
     7861 cgcgttggcc ggcgtacggg atgcccaggg caacgccata acggccggag cgctgcaggc
     7921 gcaggtcagc tacgacgtgc cggacgccac ccttccgccg ggcgccgcca cgttgcggga
     7981 gggcttcagc cttaacctcg gacaggtggg cgcgtatcgg gacgccatca cccagttcgc
     8041 ggagaccagc tccaccaagg cggtccgcca ggacggctac gggatggggt acctggagac
     8101 cttcaagatc gaccagtccg gcgtgatcac cggtgtctac accaacggct ccaaccggat
     8161 cctgggccag atcggcctgg cctccttcac gaatcccgg
//
LOCUS       DASRZS010000559         5397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_689993, whole genome shotgun sequence.
ACCESSION   DASRZS010000559 DASRZS010000000
VERSION     DASRZS010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689993"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VFH83_13675"
     CDS             complement(<1..758)
                     /locus_tag="VFH83_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487471.1"
                     /translation="MRKLASLSAALILAVVAPTIAMAQAGGSGSTAPTAGTSTTAAAS
                     GPDLSVPQVAAKAWTVGFSAFSSEGLSTGNGYLSYSLPLMILGALNGLDTHHFSAAET
                     AAIQRQAIAASMQTAEQALSSSIQSRDALLFAAQQDAATRTGADQKVGFAQARILFLT
                     SFDPSQVAVPASLPIVFKDGSSTAKLFDAPAIPLAELCASQGLDLLVAGSLREVQGYL
                     LVDVWAYDAHQDQTVFSYREAAPRDEIYSYMPTIS"
     gene            complement(758..1126)
                     /locus_tag="VFH83_13680"
     CDS             complement(758..1126)
                     /locus_tag="VFH83_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015010220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6487472.1"
                     /translation="MAVGVLIVDDLTFIKIVLREILESAGFRVIGEASNGEQAIAQYQ
                     VRRPDVVLMDITMPGMDGLTALRKIRELDPEARIIMCSALGQQQLIVKAIQMGAKDYI
                     VKPIQPNRVIGAVKKALDIS"
     gene            complement(1101..2229)
                     /gene="prfB"
                     /locus_tag="VFH83_13685"
     CDS             complement(join(1101..2159,2161..2229))
                     /gene="prfB"
                     /locus_tag="VFH83_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_148224056.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET6487473.1"
                     /translation="MLSELEQKLSSLGGQIEETWRGLDPEDLARQIAEIEKQTSAADF
                     WQNKEKAGEALTRLKALKRRYDPWSRLRSDFEDLRGIWHLAVEEKDESLEADLRTNWE
                     SLEKRFAELKVLELLGEENDSSSAFLTIHSGAGGMEACDWVSMLLRLYTRWSERKGYS
                     VDILDILEAEGGIKSVTVQVNGSYAYGYLKSETGIHRLVRISPFDSNARRHTSFASVF
                     CFPVLDDTIDVDIRPEDLRIDTYRAGGAGGQHVNKTDSAVRITHLPTGIVVQCQNERS
                     QYKNKAFAMKVLRSRLYEHYRQERDKEKEKMEQEKKEISWGNQIRSYTFQPYTLVKDH
                     RTKLEVGNIQAVMDGEIDPFIEAYLKQIWLGRNDGGRRTDR"
     gene            complement(2315..2587)
                     /locus_tag="VFH83_13690"
     CDS             complement(2315..2587)
                     /locus_tag="VFH83_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas2"
                     /protein_id="HET6487474.1"
                     /translation="MFVAVVCEYATDDHRKAAYDLLAQYGFRKVLRDAYESTSLSEVA
                     LSRLKRDLDRIADSYDGLRLYQYPVDGTLAITVLKEKKWRRLIVKV"
     gene            2737..3990
                     /locus_tag="VFH83_13695"
     CDS             2737..3990
                     /locus_tag="VFH83_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487475.1"
                     /translation="MRVTRTRAPLPLIAMLLLAPALCFAQAAAQPLAPFVSRLHAEVV
                     DYQVKLTWKDAPDVKGTYLVYRGSQPLGADTLAKAQLLGEVASGVELYVDAPPDLSPY
                     YYGVLLKDTAGKVYQLFIQFRNITSAPTAVLATAPESALAATITAIKAVPAADGISVT
                     FQSTSATRDLILFRSTSPMNTLDDILGSTAALQLDPGTTRYLCPALPGVDYWFAVMDA
                     GTYKLGKAPLVPGANSTVTPAQLPLPVGLGTASTFLSQRALPLPTLVVPFDVRTGQAV
                     SVSDVPLVPAPRRISSATEKSIGLLLQRVTPPPAAPLKPQLLVSDATPSPDGELAMLQ
                     GIVKGALMGGDTMAAEKGLLDFLSLHRAPEREARARFYLGQTYYFEDRPRDALLEFLL
                     CEDTFYQEVQRWEDACFERLETQDR"
     gene            complement(4048..>5397)
                     /locus_tag="VFH83_13700"
     CDS             complement(4048..>5397)
                     /locus_tag="VFH83_13700"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HET6487476.1"
                     /translation="VLASGGLLRGDWLERFIRMSQESAEWLQATTPGQYLKEYPPAER
                     LYVPSLSSMDMMGWALSPDVRGKYLEARKKSLEEADSSRFLVGGHFRQFLTRYPEAWL
                     MYAKMMYTHVIVNQVRGDRYKKKAAQNELWKGQCHHAYWHGGATGGIYENALRKAVYR
                     SLIEAEKITRATEIFAPSIISADYDMDNMVEYLYQGSELNAYIHARGAALVELDFLPS
                     SWNYLDTMEERGGSPTPGHRRRAFLDHFFEDDCSIDGFTAGRIRETGDFLAEPYELVD
                     LNRALPELLMRRSGSVLRDGVPCPVTIEKRFVFRPRSIDVYYRVTQPGPSVLRVPFGV
                     ELNVSLAGRSPDYGRLFLLDEGRKREIESDAVEISGVQGLLVRDVRNEVSITLSSALP
                     FRCWSLPVQTSPFSAGSGEPSFQSHCFVPLWQLELAPGEGWENHMSVGFEKTQEGRI"
BASE COUNT          909 a         1792 c         1696 g         1000 t
ORIGIN      
        1 gagatcgtgg gcatgtagga gtagatctcg tcgcgaggcg cggcctcgcg ataggagaac
       61 accgtctgat cctggtgcgc gtcgtaggcc cagacgtcca ccagaaggta tccctggacc
      121 tctcgcaggg acccggcaac caggaggtcg agaccctgcg acgcgcagag ctctgccagc
      181 gggatggccg gagcgtcgaa gagctttgcc gtggaggacc cgtccttgaa cacgatggga
      241 agcgaggcag ggaccgcgac ctggctcgga tcgaaactgg tgaggaagag gatccgggcc
      301 tgggcgaagc cgaccttctg gtccgcgccc gtccgcgtag cggcatcctg ttgggccgca
      361 aacaggagcg cgtcgcggct ctgaatggag gagctcagtg cctgctccgc cgtctgcatg
      421 gatgcggcaa tggcttgccg ctggatcgcc gccgtctctg ccgccgagaa gtggtgcgtg
      481 tccagcccgt tcagcgcgcc aaggatcatc aacggcaggc tgtagctcag gtagccgttc
      541 cccgtggaga gtccctccga agaaaaggcg ctgaacccca ccgtccacgc cttggccgcc
      601 acctggggga cgctgaggtc cgggccgctg gccgcggcgg tggtggaggt accggccgta
      661 ggcgccgtgg atcccgagcc gccggcctgg gccatcgcta tcgtgggggc gaccacggca
      721 aggatcagcg ccgccgagag actcgcgagc ttccgcatca gcttatgtcc agggccttct
      781 tcacggcccc gatgacccgg ttcggctgga tcggctttac gatgtagtct ttcgccccca
      841 tctggatggc tttcacgatg agctgctgct gccccagcgc ggagcacatg atgatcctgg
      901 cttccggatc gagctcccgg atcttgcgga gcgccgtgag cccgtccatg ccgggcatgg
      961 tgatgtccat gagcaccacg tccggacgac ggacctggta ctgggcgatt gcctgttctc
     1021 cgttggaggc ttcgccaatc acccggaagc ccgccgactc caggatctct cggaggacga
     1081 tcttgatgaa cgtcaggtcg tcaacgatca gtacgccgac cgccatcgtt ccgcccgagc
     1141 cagatctgct tcagatacgc ctcgatgaac ggatcgatct cgccgtccat caccgcctgg
     1201 atgttcccga cctcgagctt ggtccgatga tccttgacca gcgtgtaggg ctggaaggtg
     1261 taggagcgga tctggttgcc ccaggagatc tccttcttct cctgttccat cttctccttc
     1321 tccttgtcgc gctcctggcg gtagtgctcg tacaggcggg accgaaggac cttcatggcg
     1381 aaggccttgt tcttgtactg gctgcgctcg ttctggcact gcacgacaat gccggtgggc
     1441 aggtgcgtga tccgcaccgc ggagtcggtc ttgttcacgt gctggccgcc cgccccaccc
     1501 gctcgatagg tgtcgattcg caggtcctca gggcggatat ccacgtcgat ggtgtcgtcc
     1561 agcaccggga agcagaatac ggaggcgaac gatgtgtggc gccgggcgtt ggagtcgaac
     1621 ggcgagatcc gcaccaggcg gtgaatcccg gtctccgact tgagataccc gtaggcgtag
     1681 gagccattca cctggacggt gacgctcttg attccccctt cggcctccag gatatcgagg
     1741 atatctaccg aatagccctt tcgctcggac caacgggtgt agaggcgaag gagcatgctc
     1801 acccaatcac atgcctccat gccaccggcg ccagagtgga tggtcagaaa ggcggatgag
     1861 gagtcgttct cttctcccag gagctccagc accttgagct cggcgaaccg cttctcgagc
     1921 gattcccagt tcgtccgcag atccgcttcg agggactcgt ccttctcttc cacggccagg
     1981 tgccagatcc ctcgaaggtc ctcgaagtcg gatcgcagcc gggaccaggg atcgtagcgg
     2041 cgcttcagcg ccttgagccg ggtcagcgcc tctcccgcct tctccttgtt ctgccagaag
     2101 tcggcggccg atgtctgctt ctcgatctcc gcgatctgcc gggcaagatc ctccgggtca
     2161 aagtcccctc caggtttctt caatctgccc gcccagactg ctcagtttct gctccagctc
     2221 gctcagcacg gtatcccctc ccccgctcgc tttctcaaat cttctcgatc cttctcaaac
     2281 cttctcgatc cttctcaaat cttcacaatc tttctcaaac cttcacaatc agccgtcgcc
     2341 acttcttctc cttcaggacc gttatggcga gggtcccgtc cacgggatac tgatacagtc
     2401 gcagaccgtc gtacgagtcc gcgatccgat ccaggtcccg cttcagccga ctgagcgcta
     2461 cctccgagag ggaggtggac tcgtaggcat cccggagcac ctttcggaac ccgtattggg
     2521 ccagaaggtc ataggccgcc ttgcgatggt catcggtggc gtattcgcag acaacggcca
     2581 cgaacatacg gaatgcagtg tatgggtttg gcacaggcgt gtaaagcttc tctgcgggcg
     2641 cggctggccc cgccgcagag cgctgaagcc ggcgcaggga tcgccgtgta tagacaaatg
     2701 ggcgcggagc cgacaccatt ggtatagagg agaccgatgc gggtaacccg gacacgcgct
     2761 ccgcttccgc tgatcgcaat gctgctgctg gctccggcgc tgtgcttcgc ccaggcggcc
     2821 gcgcagccgc ttgctccctt cgtctcgcgg ctccacgcgg aggtggtgga ctaccaggtg
     2881 aagctgacct ggaaggacgc tccggatgtg aagggcacgt acctggtgta ccgcgggtct
     2941 cagccgctcg gcgccgacac ccttgccaag gcccagctcc tgggagaggt ggcgtccggc
     3001 gtcgagctct acgtagacgc cccgccggac ctttcgccgt actattacgg cgtgctcctg
     3061 aaggacacag caggcaaggt gtatcagctt ttcatccagt tccgaaacat cacgagcgcc
     3121 ccgaccgcgg tcctggcaac tgccccggag agcgccctcg cggcgacgat cacggcgatc
     3181 aaggccgtac cggccgcgga cggcatctcc gtgacgttcc agtccaccag cgccacccgg
     3241 gacctgatcc tgtttcgaag cacctctccc atgaacaccc tcgacgacat cctggggtcc
     3301 acggccgcgc tgcagcttga ccccggcacc acccggtacc tgtgtcccgc cctcccaggc
     3361 gtggactact ggttcgcggt gatggacgcc ggcacgtaca agctcggcaa agcacccctg
     3421 gtgccgggcg ccaactccac ggtcacgccc gcgcagcttc cgcttcccgt ggggctgggc
     3481 acggcatcga ctttcctgtc ccagcgcgcc cttccgctgc cgacgctggt ggtgcccttc
     3541 gacgtgcgga ccgggcaagc cgtctcggtc tcggacgtcc cgttggtacc ggcgccgcga
     3601 aggatctcct cagccacgga gaagtcgatc ggcctcctgc tccagcgcgt cactccgcca
     3661 cccgcggctc cgctcaagcc gcagctcctg gtctcggacg cgacgccgtc accggacggt
     3721 gagctggcga tgctgcaggg gatcgtgaaa ggcgccctga tgggcgggga cacgatggcc
     3781 gcggagaaag ggctgctcga cttcctgagc cttcatcgag cgccggagcg ggaggcccgc
     3841 gcccggttct acctcgggca gacctactat ttcgaggatc gtccccgcga cgccctgctg
     3901 gagttcctgc tgtgtgagga caccttctac caggaagtac agcggtggga ggacgcctgc
     3961 ttcgaaaggc tggagacgca ggaccggtag cccgcgccgg ccgcgagccg ttggccggca
     4021 gcggcactcc ggcttcggct ggtccgccta gatcctgcct tcctgggtct tctcgaaccc
     4081 tacgctcatg tggttctccc acccctcgcc aggggccagc tcgagctgcc acagcggtac
     4141 gaagcagtgg gactggaacg acggctcgcc ggagcccgcg ctgaacggcg atgtctggac
     4201 cggcaggctc cagcaccgga acggcagcgc ggaggaaaga gtgatggaga cctcgttgcg
     4261 gacgtcgcgg accagaagcc cctgcacgcc ggagatctcc accgcgtcgg actcgatctc
     4321 ccgtttgcga ccctcatcca gcaggaacag gcgcccatag tccggggacc gtccggcaag
     4381 ggaaacgttc agctccactc cgaacgggac ccgcagcacc gacgggcccg gctgggtgac
     4441 ccggtagtag acgtcgatgg accgggggcg gaacacgaac cgcttctcga tcgtgacggg
     4501 acagggcacg ccgtcccgaa ggacggagcc gctcctgcgc atgagcagct cgggcaacgc
     4561 ccggttcagg tccaccagtt cgtagggttc cgccaggaag tcgccggtct cccggatgcg
     4621 tccggcggtg aacccgtcga tggagcaatc gtcctcgaag aagtgatcga ggaatgccct
     4681 gcgccggtga ccgggcgtgg ggctcccgcc gcgctcttcc atggtgtcca ggtagttcca
     4741 ggacgatggg aggaagtcca gctccacaag cgccgcgccg cgcgcgtgga tgtaggcgtt
     4801 gagctccgag ccctggtaga gatactccac catgttgtcc atgtcgtagt ccgcgctgat
     4861 gatcgacggc gcgaagatct cggttgcgcg cgtgattttc tccgcctcga tcagggatcg
     4921 gtagaccgcc tttcgcagcg cgttctcgta gatgccgccg gtcgcgccgc cgtgccagta
     4981 ggcgtggtgg cactgtccct tccaaagctc gttctgagcc gccttcttct tgtaccggtc
     5041 cccccgaacc tggttcacga tgacgtgtgt gtacatcatc tttgcgtaca tcagccacgc
     5101 ttccgggtag cgggtgagaa actgccggaa atgcccgccg acaaggaacc tgcttgagtc
     5161 cgcttcctca aggcttttct tgcgggcttc caggtactta ccgcgcacgt cgggggaaag
     5221 ggcccacccc atcatgtcca tggaggagag ggagggcacg tacagccttt ccgcgggcgg
     5281 gtactccttg agatactgcc ccggcgtggt ggcctgaagc cactccgcgc tctcctgcga
     5341 catgcggatg aagcgttcca gccagtcgcc ccggagaagc ccgccggaag cgagcac
//
LOCUS       DASRZS010000560         8520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5316009, whole genome shotgun sequence.
ACCESSION   DASRZS010000560 DASRZS010000000
VERSION     DASRZS010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8520
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5316009"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFH83_13705"
     CDS             complement(<1..298)
                     /locus_tag="VFH83_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HET6487477.1"
                     /translation="MAAGTAERRIFHESWYRLAGQRLSLRSSVRARRQLFRGERWYVL
                     HEPFSDQFFRLRPPAYELLCRLGSGGTVDEVWRQAMERDPENAPGQEEVIQLLAQ"
     gene            complement(308..2170)
                     /locus_tag="VFH83_13710"
     CDS             complement(308..2170)
                     /locus_tag="VFH83_13710"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6487478.1"
                     /translation="MSTEEVTLRGGHAQHQDPYLRSLEQLHQLGRYRGEPAQFWRSFL
                     GILMSMADASAGAVLVRGAGKGAPWRVAAREPPQADGLPDRLTGGMDTVAEPCVRDGV
                     TLWGADGERRAAVHLDTGGGEELCLAALVLGRSSRLAGPEVTRRLLLAAHAPADYQAR
                     RVAVASAGQVESLAGALDLSVVLNDQNRFLPAAMAFCNEIAARLRCERVSLGWTEKGY
                     IELQAASHVDRFDRKTDAVQLLEGAMEEALDQDADVLVPADGSGGAVSRDHQSFARAQ
                     DSRYLCSLPLRVEGAPVAVCTCERNTTPFTESELQRLRLLCDLAARRLADLKRWDRWF
                     GARLAMGAKEGIGRLIGFEHTWLKVLGIILAAAVAVLLFASVKYRVTAPVELRTDDVA
                     VLSAPYDGHIDKVDVRIGDTVQEGQELLALDQTDLLLAQAQIVAQEDAYQGELAKARA
                     SYSLADMRIAQARVEQAAAQYEQIRYHLDQAIVRAPFTGVVVEGDLRQRIGLPVHQGD
                     TLFRMARIDRLYAQLRVSERDVRELANVTGGEIALASRPQESAPIALTRVEPEAVTES
                     TGNVFIVDCGFPEGPAGWWRPGMTGVAKLDVGTRRLLWVLTHRTADFLRLRLWW"
     gene            complement(2167..3000)
                     /locus_tag="VFH83_13715"
     CDS             complement(2167..3000)
                     /locus_tag="VFH83_13715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6487479.1"
                     /translation="MKNLVVPLIWFCLALSVPPSAFAQQQAARGITEPFHDVTLSVTV
                     PGRVSEILRKEGDRVQAGDEILALDRDLESLEVERRRIILDSTVELQEAKQKLATADQ
                     LLKSTRTLYASTHSVSQDELSQKELDASVAALEVQRLQDAEKQQKIELDLAQVQLSQR
                     TVAAPFDGVIVKLFVDLGDQCNPQAPVARIVDNSRCRLIVQIDAASSRGLDKGTAVRI
                     SVPSTIAPAPTQGTVEYIAPVVDPSSGLREVKILFDNPDGRVLPGVTGSLVLPQRGST
                     N"
     gene            3202..4776
                     /locus_tag="VFH83_13720"
     CDS             3202..4776
                     /locus_tag="VFH83_13720"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6487480.1"
                     /translation="MGSKDRTVARKARAFGVACLALAATSAAAQAIPLPGRMVGLTEF
                     LREVVRTNPSVREARLQWLISTGQARAEWGLYEPAFDGSYQQDGLRQQNTSLQQFQQL
                     GQQVYDQKNDAFTVGVGGALPTGATYNLKASVTRLQDTYVEQGQFQSFVGVTANQPLL
                     KGFTNGAPFASIRTAFEDRIIAFHEYRRQIAATVSQAETAYWNLAFAQEVSRMSAASV
                     KVAQDLLEVSKAAASAGRMSDLDVRQAESELSARKSAQEDSDLSLNESQRQLRLLLGD
                     QSDLSKGTIVAADPIVSDGAGSWDPGGQTDALLEWARRAQPDYMIASEQAHRESILVG
                     YQKDQALPELNLTGSYGYSGLGTTPQDSLSSLQTQAYPNWTVGVELKVPLLLGVRQRN
                     NLEVEQLKSELASVRLQATGQQMEDAIASLARSVGTLLSHIENQRAVAEAKSHLLDVQ
                     NAGLRAGTASLLDVYGAEDALRQAQQAELDAVVRYRQAMMDLERTSGTVLRDNDLEKL
                     QDGQVVLDEELRTGSH"
     gene            4797..5705
                     /locus_tag="VFH83_13725"
     CDS             4797..5705
                     /locus_tag="VFH83_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HET6487481.1"
                     /translation="MSDQERRAELYSLLGDLPARDHPLSARTVSVETRESYILEKLLF
                     DLNGREAVPAYLARPKAVRGRAPAVLYNHAHGGRYDIGKEELLSGREAFFQKPSYAEV
                     LTGMGYCVLCVDTWLFGERGGRTESEVFKEMLWSGQVLWGMMVYDGIRALDYLLSRPE
                     VDPSRVATMGLSMGSTMAWWLAALDPRIKVCVDLCCMTDYQALVATRGLDRHGIYYYV
                     PGLLKHFSTAQINSLIAPRPHLATAGRYDLLTPGEGLDRIDRELKRVYGEAGAADAWT
                     LTRYDCGHMETAAMRQEVVEYLKRWL"
     gene            complement(5726..6373)
                     /locus_tag="VFH83_13730"
     CDS             complement(5726..6373)
                     /locus_tag="VFH83_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET6487482.1"
                     /translation="MKIAVLGTGTVGTTIASKLVSLGHEVMMGSRDAANAKGAAWATK
                     VGGTARHGTFSQAATFGEIVFNCTAGTGALQALEAAGAENLGDKVLIDVSNPLDFSKG
                     MPPALLFPGNDSLGERIQQAFPRSRVVKTLNTITASLMVEPGRVSGEHDVFVSGNDAQ
                     AKAFVSRILREWLGWKRVVDLGDIKTSRGAEAYVLFWVHLWGSLGTADFNISVRR"
     gene            complement(6404..7921)
                     /gene="malQ"
                     /locus_tag="VFH83_13735"
     CDS             complement(6404..7921)
                     /gene="malQ"
                     /locus_tag="VFH83_13735"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET6487483.1"
                     /translation="MTGAGRRSSGILLHITSLPGRFAIGDIGPCAVRFLDWLRDAGQR
                     LWQVLPLGPTGYGNSPYACFSSFAGNPLLVSPEALASWGVVSANDVSEAPPPSARVDY
                     AAVMAWKLPLLRNAADAFLSAAPATLRSRYEAFRSQESWWLDDYSLFAGIKSRYPGAW
                     NRAWPRELALRHPDAVDAFRREHREETERQRVIQFFFFSQWADLRAEAARREIAIIGD
                     LPIFAAPDSADVWSHPGHFLLDAEGMPTVVSGVPPDYFAATGQLWGNPLYDWDELARQ
                     GFRFWVDRIRAARRLFDLIRVDHFRGFDACWTVPASAPTAESGRWMPVPGAALFETLQ
                     RELGDLPILAEDLGVITPSVVELRERFKFPGMRILQFAFDQAEAGRLDATNRFLPHNY
                     TPDSVVYTGTHDNDTTRGWFKTRTAAERDYLSRYAATTEPEVEWKLIRMAMASVAGRA
                     IVPLQDVLGLGGEARMNTPGTSGEANWSWRAREDALDPATAVRLRELATLFGRCP"
     gene            complement(7918..>8520)
                     /locus_tag="VFH83_13740"
     CDS             complement(7918..>8520)
                     /locus_tag="VFH83_13740"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6487484.1"
                     /translation="VDYDHQVGAMKAVTHLIELGHRDIAIIGGKPETRLYSPIGKDRL
                     KGYRMAMALAGIPVRKDFIMSGEWTSADAMASAHRLLALKRPPTAIFATSDVQAAGVL
                     MAARERRLSVPGDLSVVGYDNIPFAEFLSLTTMWQRHDQLARNAANLLLEELRTGVRA
                     QERVVLQPELVIRASSGPPGGVEPRLRGGVEPRPRAGRQP"
BASE COUNT         1428 a         2814 c         2896 g         1382 t
ORIGIN      
        1 gctgggcaag gagctggatg acttcttcct ggccgggcgc gttctcggga tcccgctcca
       61 tggcctggcg ccagacctcg tccacggtgc cgccgctccc cagccggcag agaagctcat
      121 aggccggcgg tctgagtcgg aagaactggt cgctgaaggg ctcgtgcagc acgtaccagc
      181 gttccccccg gaagagctgg cggcgagccc gcacgctgga cctcagggag agccgctggc
      241 cggcgagccg gtaccacgac tcatggaaga tcctgcgctc cgccgtaccg gccgccacgg
      301 gaggtcctta ccaccagagc cgcagccgca ggaagtccgc ggtgcggtgg gtgaggaccc
      361 agaggagccg gcgggtccca acgtcgagct tggccacgcc cgtcatcccc ggcctccacc
      421 agccggccgg gccttcgggg aagccgcagt ccacgatgaa cacgttcccg gtgctctcgg
      481 tcacggcctc gggttccacg cgggtgagcg caatgggagc cgattcctgg ggtcggctgg
      541 ccagggcgat ctccccgccg gtgacgttgg caagctcacg gacgtcccgc tcgctcaccc
      601 tgagctgggc gtagagccga tcgatcctgg ccatgcggaa gagggtgtct ccctgatgga
      661 cgggcagccc gatccgctgc ctaaggtctc cctccaccac cacgcccgtg aatggggccc
      721 ggacgatggc ctggtccaaa tggtagcgga tctgctcgta ctgggccgcc gcctgctcca
      781 cccgcgcctg ggcgatgcgc atgtccgcaa gcgaatagga tgcgcgcgcc ttcgccagct
      841 cgccctggta ggcgtcctcc tgggccacga tctgcgcctg ggccaggagg aggtccgtct
      901 ggtccagggc aagcagctcc tggccctcct ggaccgtgtc tccgatgcgg acgtccacct
      961 tgtcgatgtg cccgtcatag ggcgcgctca acaccgccac gtcatcggtg cgaagctcga
     1021 ccggcgcggt cacgcgatac ttcacgctgg cgaagagcag gaccgcaacg gcggcagcca
     1081 ggatgatccc caggaccttg agccacgtgt gctcgaagcc gataagacgg cctattcctt
     1141 ccttggcgcc catggccagc cgggccccga accaccgatc ccaccgcttg aggtcggcca
     1201 gtctccgagc ggccagatcg cagagaaggc gcaggcgctg caactccgat tccgtgaacg
     1261 gggtggtatt ccgctcacag gtgcagacgg ccaccggagc tccctccacg cggaggggga
     1321 gggagcacag gtagcgggag tcctgggcgc gggcaaagct ctggtggtcc cgggacacgg
     1381 ctcccccgga tccgtccgcg ggcaccagca cgtccgcgtc ctgatcaagg gcctcctcca
     1441 tggctccttc caggagctga accgcatcgg tcttgcggtc gaaccggtcg acgtggctgg
     1501 cggcctgcag ctcgatgtac cccttctcgg tccagccgag actcactcgt tcgcagcgca
     1561 gccgggccgc gatctcgttg cagaacgcca tggcggccgg aaggaagcga ttctggtcgt
     1621 tcaggaccac cgacagatcc aacgcgccgg caaggctctc gacctggccc gcggaagcca
     1681 cggcgacgcg ccgcgcctgg tagtcggccg gggcgtgcgc cgccaggagg aggcgtcgcg
     1741 tgacctcggg gccagccagg cggctgcttc ggcccaggac caacgccgcc agacacagct
     1801 cctccccgcc tccggtgtcc agatggaccg cggcgcgtcg ttctccgtca gctccccaga
     1861 gcgtgacgcc gtcccgaacg cacggctcgg ccacggtatc catcccgccg gtgagccggt
     1921 ccgggaggcc atccgcctgg ggcggctccc gggccgcgac ccgccatgga gcgcccttcc
     1981 cggcgccacg caccaggacg gcgcccgcgg aggcgtccgc catggacatc aggatgccca
     2041 ggaaggatcg ccagaactgg gcgggctctc cacggtagcg gccaagctga tggagctgtt
     2101 cgagggaccg aagataaggg tcctggtgct gggcatggcc gccccgaagg gtgacctctt
     2161 ccgtgctcaa ttggtggatc ctctctgggg caggaccagg gagccggtca ccccgggcag
     2221 aacccggcca tcgggattgt cgaagaggat cttcacctct ctcagtccgc tggaggggtc
     2281 caccacggga gcgatgtact ccaccgtgcc ctgggtaggc gccggagcga tggtggaggg
     2341 caccgagatg cggaccgctg tgcccttgtc cagaccccgg gaggaggcgg catcgatctg
     2401 cacgatcagc cggcagcggc tgttgtccac gatccgggcc acgggcgcct gggggttgca
     2461 ctggtccccc agatcgacga acagcttgac gatcaccccg tcgaacgggg ctgcgacggt
     2521 cctctgggag agctggacct gggccaggtc gagctcgatc ttctgctgct tctcggcgtc
     2581 ttgcaggcgc tgcacctcca gggcggccac gctggcatcc agctccttct ggctgagctc
     2641 atcctgagac accgaatgcg tggaggcgta gagggtccgg gtgcttttca ggagctggtc
     2701 ggcggtggcg agcttctgtt tggcttcctg gagctccacg gtgctgtcca ggatgatccg
     2761 acgtcgctcg acttccagcg attccaggtc tctgtcgagc gccaggatct cgtcgccggc
     2821 ctggacccga tccccttcct tgcggaggat ctcggaaacc cgacccggaa cggtcacgct
     2881 cagggtgacg tcatggaagg gctcggtaat gccgcgcgcc gcttgttgtt gtgcgaaggc
     2941 tgaaggtggg acggagagcg caagacaaaa ccatatcaat ggcacgacga gattcttcac
     3001 gatccgctcc tggccggaga gcccctcatc ccatggggta actgtgctca aaggtaactc
     3061 tgtgcgtcgc tggtctgcaa atcctttttt tgattgactt cggcgcgggt ttcctcacct
     3121 ttttatgggg ggcgtggttg gccggattcc gtcacgggcg cgtggccgaa caccctccgt
     3181 ttttccaatc gggaggaagc aatggggtcc aaggatcgaa cggtggcccg aaaggctcgg
     3241 gcctttgggg tggcatgcct tgcgttggcc gcgacgagtg ccgcggctca ggccatcccg
     3301 ctccccggtc ggatggtcgg cctcacggag ttcctgaggg aagtggtccg gaccaaccca
     3361 tcggtccggg aggcccggct ccagtggctc atcagcacgg gacaggcgcg cgcggaatgg
     3421 gggctgtacg aaccggcgtt cgacggtagc taccagcagg acgggctgcg gcagcagaac
     3481 accagcctcc agcagttcca gcagctcggc cagcaggtct acgaccagaa gaacgacgcc
     3541 ttcacggtcg gggtcggggg cgcgcttccc accggggcta cctacaacct gaaagcgtcc
     3601 gtgacacggc tgcaggacac ctacgtggag cagggccagt tccagtcttt tgtgggcgtg
     3661 acggcgaacc agcccctgtt gaagggcttc accaacggcg cgccgtttgc atccattcga
     3721 acggcgttcg aggatcggat cattgccttt cacgaatacc ggcggcagat cgccgccacg
     3781 gtttcccagg ccgaaacggc gtactggaac ctggccttcg cccaggaggt ctcccggatg
     3841 tcggcggcgt cggtgaaggt cgcccaggat cttctggagg tgagcaaggc ggcggccagc
     3901 gcagggcgga tgtcggacct ggacgtgcgt caggcagagt ccgagctgtc ggcgcggaag
     3961 tccgcccaag aggattcgga tctctccctg aacgagtccc agaggcagct ccgccttctc
     4021 ctgggagacc agagcgacct gtccaagggc accatcgtcg ccgcggatcc tatcgtgtcg
     4081 gacggtgccg ggagttggga cccgggcggc cagaccgacg cgctcctgga atgggcgcgg
     4141 cgagcccagc cggactacat gatcgcctca gagcaggccc atcgggaatc gatcctggtg
     4201 gggtatcaga aggaccaggc acttccggag ctcaacctca cgggcagcta cggctatagc
     4261 ggtctcggga ccacgcccca ggactccctg agcagcctcc agacccaggc ctaccccaat
     4321 tggaccgtcg gtgtcgagct caaggtgcct ttgctccttg gcgtccgcca gcggaacaac
     4381 ctggaagtgg aacagctcaa gagcgagctg gcgtcggttc gcctccaggc gaccgggcag
     4441 cagatggaag acgccatcgc ctcgctggcc cgaagcgtgg ggaccctcct ttcccacatc
     4501 gagaaccagc gagccgtcgc ggaggcaaag agccacctgc tggacgtgca gaacgccggg
     4561 cttcgagcgg gaaccgccag cctgctggac gtgtatggag ccgaggatgc gctccgccag
     4621 gcccagcaag cggagctgga cgcggtggtt cggtatcggc aggccatgat ggacctggaa
     4681 cgcacgagcg ggaccgtgct tcgggacaac gatctggaga agctccagga cggtcaggtg
     4741 gtgctggacg aggagctcag gaccggctca cactgacgcg aagacggagg gaatcgatgt
     4801 ccgatcagga gcgcagggcc gagctataca gcctgttggg cgatcttccg gcccgggacc
     4861 atccgctgtc cgcgcggacc gtttccgtgg agacccggga gtcctacatc ctggaaaagc
     4921 tccttttcga tctcaacggc cgggaggcgg ttcccgccta tctggcccgg cccaaggccg
     4981 tacgaggccg cgcgcccgcc gtgctgtaca accatgccca tggcgggcgg tacgacatcg
     5041 gcaaggagga gctcctctcg ggacgggagg cctttttcca gaagccgtcg tacgcggaag
     5101 tgctgacagg catgggctac tgcgttctct gcgtggacac gtggctcttc ggggagcgtg
     5161 gcggaaggac cgagtccgag gtcttcaagg agatgctctg gtccggccag gtgctctggg
     5221 ggatgatggt ctatgacggc atccgtgcct tggactatct gctgtctcgt cccgaggtgg
     5281 accccagccg cgtcgccacg atggggcttt ccatggggag caccatggcg tggtggctgg
     5341 ccgcgttgga tccccggatc aaggtctgcg tggacctgtg ctgcatgacg gactatcagg
     5401 cgctggtcgc gacacgaggc ctggaccggc acgggatcta ctactacgtc cccggtctcc
     5461 tcaagcactt ctcgacggct cagatcaact ccctgatcgc accgcgtccc cacctcgcca
     5521 cggcaggtcg ctacgatttg ctgacccctg gggaaggatt ggatcggatc gaccgggagc
     5581 tgaagcgtgt ctacggggaa gcgggagcgg cggacgcctg gaccctgacc cggtacgact
     5641 gcggccatat ggagaccgcg gccatgcgcc aggaggtcgt ggagtacctc aagcgctggc
     5701 tatagccgcc aaaggtcgat catcctcagc ggcggacgct gatgttgaaa tccgcggttc
     5761 cgagggaacc ccagagatgc acccagaaga gcacgtaagc ctccgcgccg cgggaggtct
     5821 tgatatcccc caggtccacc acccgtttcc aaccgagcca ctcccgcagg atccgggaaa
     5881 cgaaggcctt ggcctgggca tcgttgccgc tcacgaagac gtcatgttcg ccggagaccc
     5941 ttcccggctc caccatgagc gacgcggtga tggtgttcag ggtcttcacc actctggatc
     6001 tcgggaaggc ctgctggatc cgttccccca gggagtcgtt gccggggaag aggagcgctg
     6061 ggggcattcc ctttgagaaa tccaggggat tcgagacgtc gatcaggacc ttgtcaccga
     6121 ggttctcggc gcccgccgcc tccagggcct gcagggcgcc cgtgcccgcg gtgcagttga
     6181 agacgatctc tccaaacgtc gccgcctggg agaaggtccc atgccgggcg gtcccaccga
     6241 ccttcgttgc ccacgcggcc cctttcgcgt tcgccgcgtc gcgcgatccc atcatcactt
     6301 cgtggcccag cgagaccagc ttcgaagcga tcgtggttcc caccgttccg gtccccagca
     6361 ctgcgatctt catccctgcc tccgcatcag acgaatatcg ccatcagggg caccgcccga
     6421 acagcgtggc gagctctcga agccgcaccg ccgtggctgg gtccagggca tcctcccgcg
     6481 cccgccagga ccagttggcc tctccgctgg ttcccggagt gttcatccgg gcctcgccgc
     6541 ccagcccaag aacgtcctgg agcggcacga tggcgcggcc ggcgacggaa gccattgcca
     6601 tgcggatcag cttccattcg acttcgggct ccgtggtggc cgcgtaccga ctcaggtagt
     6661 ctctctccgc ggcggtccgc gtcttgaacc agccccgagt ggtgtcgttg tcgtgggtgc
     6721 cggtgtagac cacggagtca ggggtgtagt tgtggggaag gaagcggtta gtggcgtcca
     6781 accttccggc ttccgcctga tcgaaggcga actggaggat gcgcatgcca gggaacttga
     6841 agcgctctcg gagctcaacc acggacggcg ttatgacccc caggtcttcg gcgaggatgg
     6901 ggaggtcccc cagctctcgc tggagcgtct cgaagagcgc cgcgcccggc accggcatcc
     6961 agcggccgga ctccgcggtc ggcgccgatg cggggaccgt ccagcacgcg tcgaaacctc
     7021 ggaagtggtc aacgcggatg aggtcgaaga ggcgccgcgc ggcgcggatg cgatccaccc
     7081 agaaccggaa tccctggcgg gccagctcgt cccagtcgta cagcggattg ccccagagct
     7141 gaccggttgc cgcgaagtag tccgggggaa cccccgacac gaccgtgggc atgccctccg
     7201 cgtccaggag aaagtgtccg ggatgtgacc atacatccgc gctgtcgggc gccgcgaaga
     7261 tcgggaggtc cccgatgatc gcgatctccc gtcgggcggc ctcggcgcgc agatccgccc
     7321 actgggaaaa gaagaagaac tggatgaccc gctggcgttc cgtttcttcc ctgtgctcgc
     7381 ggcggaacgc atccacggcg tcggggtggc gcagggcgag ctcacggggc cacgcccggt
     7441 tccaggcgcc ggggtaccgg ctcttgatcc ccgcgaacag ggagtagtcg tccagccacc
     7501 aggactcctg ggaccggaaa gcctcgtagc ggctgcgcag cgtggccggc gccgcgctca
     7561 ggaaggcgtc cgcggcattg cgaagcagcg ggagcttcca ggccatgacc gccgcgtagt
     7621 cgacccgggc ggagggaggc ggcgcctcgg aaacgtcgtt cgccgaaacg actccccagg
     7681 acgccagggc ctcggggctc accaggagcg ggttgccggc aaacgaggag aagcaggcgt
     7741 atggggagtt gccataaccc gtcggcccca acgggagcac ctgccagaga cgctgaccgg
     7801 cgtcccgcag ccaatccagg aaccggaccg cgcaagggcc gatgtcgcct atcgcgaagc
     7861 gtccgggaag ggaggtgatg tgcaggagga ttccgctgga ccggcggccg gcaccggtca
     7921 tggctgccgg ccggccctgg ggcggggctc tacgccgcct ctaaggcggg gctctacgcc
     7981 gcccggtggt ccgctggagg cgcggataac cagctccggc tggagcacta cacgctcctg
     8041 ggcgcggacg cccgtgcgca gctcttccag cagcaggttg gccgcgttgc gggcaagctg
     8101 gtcgtggcgc tgccacatcg tggtcaggga gaggaactcc gcgaacggaa tattgtcata
     8161 gcccaccacg gagagatctc ccggcaccga aagccgccgc tcgcgggccg ccatcagcac
     8221 gccggcggcc tggacatccg aggtggcgaa gatcgcggtg ggtggtctct tcaaggccag
     8281 cagccgatgg gcactggcca tggcgtccgc gctggtccac tccccgctca tgatgaagtc
     8341 cttccggacc gggatgcccg ccagggccat cgccatccgg tagcccttga ggcggtcttt
     8401 cccgatgggt gagtagagac gggtctcggg ttttccgccg atgatggcga tgtcccggtg
     8461 ccccagctcg atgaggtggg tcaccgcctt catggcgcca acctggtggt catagtccac
//
LOCUS       DASRZS010000561         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5693628, whole genome shotgun sequence.
ACCESSION   DASRZS010000561 DASRZS010000000
VERSION     DASRZS010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5693628"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..997)
                     /locus_tag="VFH83_13745"
     CDS             complement(65..997)
                     /locus_tag="VFH83_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HET6487485.1"
                     /translation="MSDAEDLHLEDEPEPEEAPRPSVPEAPSDGRPVWRVKIHYSSST
                     EYCRTPPGMEVGPGSMVVVPTRYGNELGRVLGEEMDPSAVSGEVRTIVRLATPADLSR
                     VQENDMKEERAYEVCREKISVRNLEMKLVSAHYLLDEQKILFFFTAESRVDFRELVKD
                     LVSVFKCRIELRQIGVRDEARVVGGLGICGRALCCNAVTDRLRPVSIKMAKEQNLTLN
                     SMKISGPCGRLLCCLSYEVDVYREVRQALPPVGTRVRYDGEEFRVQELNVLGRRARFL
                     GESGRVLDVGFESLSRNAQTQAWELHLRGGSQTG"
     gene            complement(1049..1531)
                     /locus_tag="VFH83_13750"
     CDS             complement(1049..1531)
                     /locus_tag="VFH83_13750"
                     /inference="COORDINATES: protein motif:HMM:NF015822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HET6487486.1"
                     /translation="MARIDSLGESFQYPVPEKKKAGKKDKTRLPLFSRMVEEAQTEEA
                     DEQDLTDGGGRRTVEQLLDAVFASGDRLKKLPTLELIKDYRQKVKAFLKYAIQHSITV
                     EETTSGSNVLRRKRFTVLRVIDEKLEALAASVLSAQREQLSVLAQIDEINGLLVNLVS
                     "
     gene            complement(1637..1969)
                     /locus_tag="VFH83_13755"
                     /pseudo
     CDS             complement(1637..1969)
                     /locus_tag="VFH83_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246075.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
     gene            complement(1975..>2988)
                     /locus_tag="VFH83_13760"
     CDS             complement(1975..>2988)
                     /locus_tag="VFH83_13760"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET6487487.1"
                     /translation="ADRFGTWLRRIWVSTLNFFKYVIQKGKQRFTVMLIPHSEKKIFN
                     FQISFFTLFFVTLTLGIVLVGFFLLATNFTYTNEQLTQTTQALKASESTVESLKDEVA
                     GVRQANRSFKTSLDNVVSVIQNQDLAAAAGDSITGIASDFLATPETDAGGLREISDLK
                     SMVSLMQDSVGPLNSVGKLLSTYANFIQKAPTLWPLKGGVGYVTTGFEWTIHPFTHLG
                     YFHTGIDIAWAQGTPILAAADGKVIESGRDEELGNYVIIQHSYGFTTRYGHMSAFAGK
                     HKGDRVNRGDVIGYVGTTGLSTGPHLHYEVRLGSNYVNPIDFLIVPPSIVPQLSGTPR
                     SSD"
BASE COUNT          504 a          975 c          951 g          558 t
ORIGIN      
        1 cgccgactga cctgccttat cgcgccgtag gggccccgcg aaaatccggc ggggctcctc
       61 ttccctaccc ggtctgggat cctccgcgca ggtgcagctc ccaggcctgg gtctgcgcgt
      121 tgcggctcag cgactcgaac cctacgtcca gaaccctgcc gctctctcca agaaaccggg
      181 cgcgccgacc caggacgttg agctcctgga ccctgaactc ttctccgtcg taccgtaccc
      241 gcgtccctac cggcggcagc gcctgacgaa cctcccgata gacgtccact tcgtacgaga
      301 gacagcacag aaggcgtccg caggggcccg agatcttcat cgagttgagg gtgaggttct
      361 gctccttcgc catcttgatg gagaccggcc gaagacggtc ggtcaccgcg ttgcagcaga
      421 gcgcccggcc gcagatgccg agaccgccga cgacacgggc ttcgtctcgg actccaatct
      481 ggcgaagctc gatacgacac ttgaacacgg agaccaggtc ctttaccagc tcgcggaagt
      541 ccacgcggct ctccgccgtg aagaagaaga ggatcttctg ctcgtccagg aggtagtggg
      601 cgctcaccag cttcatctcg aggttgcgca ccgagatctt ctcacggcag acctcgtagg
      661 cgcgctcctc cttcatgtcg ttctcctgca cccgggagag gtcggccggg gtggccaggc
      721 ggacgatggt gcgcacctcc ccgctcaccg cggagggatc catctcctcc ccgagcacgc
      781 ggcccagctc gttgccgtag cgggtgggga ccaccaccat ggagccggga ccgacctcca
      841 tcccgggcgg cgtacgacag tactccgtgg agctcgagta gtggatcttg acccgccaaa
      901 ccggccggcc atcggacgga gcctcgggga cggagggccg gggtgcctct tcaggctccg
      961 gctcatcttc caggtgcagg tcctcggcat cactcacgga acacactcct ctaccgacca
     1021 cgcggaagcg gaaggggctt tcacgggctc acgagaccag gttcacgagc aggccgttga
     1081 tctcgtcgat ctgtgccagc acggagagct gctcccgctg cgccgagagc accgacgcgg
     1141 ccagcgcctc cagtttctca tcgatgaccc gaaggacggt gaagcgcttg cgtcgtagca
     1201 cgttggatcc ggaggtcgtc tcctccacgg tgatgctgtg ctggatcgcg tacttcagga
     1261 aggccttcac cttctggcgg tagtccttga tgagctccag ggtgggaagc ttcttcagcc
     1321 gatcgccgga ggcgaacacg gcatccagga gctgttccac cgtgcgccgg ccgccgccgt
     1381 cggtcaggtc ctgctcatcc gcttcctcgg tctgagcctc ctcgaccatc cgggagaaca
     1441 gcggaagccg ggtcttgtcc tttttgcccg cctttttctt ttccgggacc gggtactgga
     1501 aagactcccc aagggagtcg atgcgcgcca tgagaaacgg tcagcgcttc tgcccctgga
     1561 gcgcggcttc cttggccgga aacgcaacct gggctcgctg cccgttgggt ccgacgcgag
     1621 gcggctccga ggagggcggc gtgatcttgc aggagccgtt gaagatgacc ccttcctcga
     1681 tgatcaggcg aggcgtggtg atgttgccca ggaccagccc ggtggagagg atgatgacgc
     1741 gctccgtgga caccacctcc ccgcggagga cgccgccgat cacgacggtc cccgcctgga
     1801 gggtgcactc cgcccgaccg ttcctgccga ccaggacctt ccccttggtg cggatcacgc
     1861 cggtgaagtc cccatcgatc cgcagcaggc cgttgaggtc gaactccccc ttgaaccggg
     1921 tgccttcccc gatgatcgag ttgacaaagg cttcgtccgt gccgcgcagg tcgctcaatc
     1981 gctgctcctt ggagttcccg agagctgggg tacgatgctt ggcggcacga tcaggaagtc
     2041 gatggggttg acgtagttgg agcccaggcg gacctcgtag tgcaggtgcg gtcccgtcga
     2101 gagacccgtg gtcccgacgt acccgatcac gtctccccgg ttgacccggt ctcccttgtg
     2161 cttgcccgca aaggcggaca tgtgcccgta gcgggtcgtg aagccgtagc tgtgctggat
     2221 gatgacgtag ttgcccaact cctcgtcccg gccgctctcg atgaccttgc cgtccgcggc
     2281 ggccaggatg ggcgtgccct gagcccaggc gatgtcgatg ccggtgtgga agtaccccag
     2341 atgcgtgaac gggtggatgg tccactcgaa cccggtggtc acgtacccga cgccgccctt
     2401 gagcggccag agcgtcggcg ccttctggat gaagttggcg tacgtggaga gcagcttccc
     2461 gacggagttc agcggtccta cggaatcctg catgaggctc accatgctct tcaggtcgct
     2521 gatctctcga agcccgccgg cgtcggtctc cggggtggcc aggaagtccg acgcgatccc
     2581 ggtgatgctg tcccccgcgg cggccgcgag gtcctggttc tggatcacgc tcaccacgtt
     2641 gtccaggctg gtcttgaagc tgcggttggc ctggcgcacg ccggccactt cgtccttgag
     2701 actctccacg gtggactcac tcgccttgag ggcctgcgtg gtctgggtga gctgctcgtt
     2761 ggtgtaagtg aagttggtgg ccagaaggaa gaaaccgacc agaacgattc cgagggtcag
     2821 cgtgacgaag aacagggtga agaaggagat ctggaagttg aagatcttct tctccgaatg
     2881 ggggatcagc atcaccgtga agcgctgctt ccccttctga atgacatatt tgaagaaatt
     2941 gagcgtcgaa acccagatgc gccgcagcca cgtcccgaac ctgtcagc
//
LOCUS       DASRZS010000562         3188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2222005, whole genome shotgun sequence.
ACCESSION   DASRZS010000562 DASRZS010000000
VERSION     DASRZS010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222005"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1230
                     /locus_tag="VFH83_13765"
     CDS             184..1230
                     /locus_tag="VFH83_13765"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6487488.1"
                     /translation="MADEVRIGIVGARFAGSFHAECWSTTPNARVVAVADRDESARTP
                     FAQKHAINRIYDDYRGILADPQVDLVDICVPNFLHAEVASAALRAGKDVVCEKPFATT
                     LADGQAVLAACAETGHRFFYAEDWLFAPALVRAKAIMDEGGIGKPLYYRGKESHSGSH
                     SPFAQKIRFCGGGSVIHLAVHPIGLFQGLLGVPRTIVGRCSGGGEANLIHTEAEGEDW
                     GMGILSYGGGTQAVVEGNYITAGGMEDVIEIYGSEGVLRVDLTFGSPLTVYSRKGYGY
                     AMEKADFTHGWTRPAVDENASLGYRDELSHFIGCLLGKTDQARGTTARDGYNVLRIVD
                     AIYRSHREGTAITL"
     gene            complement(1227..>3188)
                     /locus_tag="VFH83_13770"
     CDS             complement(1227..>3188)
                     /locus_tag="VFH83_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487489.1"
                     /translation="CLEQRGIYLQLDLFCYRGFLPADGLDYPDDLMKYFKQASVYNRR
                     LMELHKEYATAYLTHRNPYTALRPVEDPAVALVQVMNEDGIFWYEGRDPVGLPSYRKE
                     LDRRFGWYLLAKYGSRAALDAAWTSAAGVHRLEPDEDPSLGTVRRLPHLEGTQMYVGE
                     DPARYADYTEFLARTQLDFCREMRDHLLDLGVRCPINVTNHAQGAADIYSLDRYSDVT
                     MDNAYWNHPDTVRQDRPRYHDLLMVENDPRRTVVDSPFKLNLVTRLNHDRVAGKPFIA
                     GEWNILYGTEFRSDALPMVAAYAALQDWDGMVLYAYHHADTLAQYDEQRLDGPFNLYN
                     DPAVWGQVGLCSFAFQEGVVSPGRNSLEVCYSDRDLYAVPRNWIAPYGYACYVSRVAA
                     TFIRERYDGNADVALASGNTPTGDYTRARHGMVFARSRFVDGAQKHDGLRRFLELHRQ
                     ASDRIEVREDGERFDEDYRTYSRILDTAMKRWNLLRPGQGLVEDGALVSDTNELSFDF
                     ARGIFRISAPRLKTASGNIQGDVSLGAWRFHVRNPRMTISLLSLDGIETDHARHLLVV
                     AVGWCGNPDMAFEKEPDGSRVLVDAGHGPVSIDALEGSMTAPRPVSVYPLSPEGARAA
                     PLAGPVQFTLGEPATIYFEVEEPRAESVR"
BASE COUNT          555 a         1010 c         1074 g          549 t
ORIGIN      
        1 agcatctcgg cgagaccgag atcgggacac gagaagctca tgaacgagta tttcaagctc
       61 acgcgatcac cccttggatc cgcttcgatc cgcgcccaga aagcaaggct cggtggcggt
      121 ttctcttgac tgccctggca gcccattcta tacctttcgg gtttccggaa gaagggagcc
      181 aggatggcgg atgaggtgag gatcgggatc gtgggcgcac ggttcgccgg atcgttccac
      241 gcggagtgct ggtcgacgac cccgaatgcc agggtggtgg cggtggcgga ccgggacgag
      301 tcggcgcgga cgcccttcgc ccagaagcac gcgatcaacc ggatctacga cgattaccgc
      361 gggatccttg ccgaccccca ggtagacctc gtggacatct gcgtccctaa cttccttcac
      421 gccgaggtgg cctccgccgc cttgagggcc ggtaaggacg tggtgtgcga gaagcccttt
      481 gccacgacgc tggcggatgg gcaagccgtg ctggccgcgt gcgcggaaac cggccatcga
      541 ttcttctacg ccgaggattg gctgttcgcc ccggcgctgg tccgagccaa ggcaatcatg
      601 gacgagggag ggatcggaaa gccgctgtac tacagaggca aagaatcgca cagcggctcc
      661 cattcgccct ttgcccagaa gattcgcttc tgcggcgggg gcagcgtcat ccacctggcc
      721 gtgcatccca tcgggctctt ccagggtctg ctgggcgtgc cccggaccat cgtgggccgc
      781 tgctccggcg gcggagaggc aaacctcatc cacacggagg ccgagggaga ggactggggc
      841 atggggatcc tcagctatgg cggcgggact caggcggtgg tggaaggcaa ctacatcact
      901 gcgggcggaa tggaagacgt gatcgagatc tacggcagcg agggagtgct gcgcgtggat
      961 ctcacgtttg gcagccccct caccgtatac tcccgcaagg gctacgggta cgccatggag
     1021 aaggcggatt tcacccacgg gtggacccga ccggccgtgg atgaaaacgc cagcctcggc
     1081 tatcgggacg agctttcgca cttcatcggt tgtctgctcg gaaagacgga ccaggccagg
     1141 gggaccacgg cgcgagatgg atacaacgtg cttcggatcg tcgacgccat ctatcgttct
     1201 caccgggagg gcacggccat cacgctctag cgaacgctct ccgcccgtgg ctcctccacc
     1261 tcgaagtaga tggtggccgg ctctccgagg gtgaactgga ccggaccggc cagcggcgcc
     1321 gctcgggcac cctccggcga cagggggtag acggagaccg gccgcggcgc ggtcatgctg
     1381 ccttccagag cgtcgatgga tacgggccca tgcccggcat ccaccaggac ccggctcccg
     1441 tccggctcct tctcgaacgc catgtccggg ttgccgcacc agccaaccgc gacaaccaaa
     1501 aggtggcgcg cgtggtccgt ctcgatcccg tccagcgaga gcagggagat cgtcatgcgg
     1561 ggattgcgca cgtggaagcg ccaggccccc agagagacgt cgccctggat gttccccgat
     1621 gcggtcttca ggcgaggggc ggagatccga aagatcccgc gcgcgaaatc gaaggaaagc
     1681 tcgtttgtgt cggacaccag cgcgccgtcc tccaccagcc cctgaccggg gcgcaggagg
     1741 ttccatcgct tcatggccgt gtccaggatg cgggaatacg tccggtagtc ttcgtcgaac
     1801 cgctcgccat cctcacggac ctcgatccgg tccgaggcct ggcgatgcag ctccaggaat
     1861 cgccgcaggc cgtcgtgttt ctgcgcgcca tccacgaacc ggcttcgagc gaataccatc
     1921 ccgtggcgcg cccgcgtgta gtctcccgtg ggcgtgttgc cgctggcaag cgccacatcc
     1981 gcgttgccgt cataccgctc gcggatgaag gtcgccgcga cgcgggagac gtagcaggcg
     2041 tacccgtacg gcgcgatcca gttccgcggg accgcataca ggtctcgatc cgagtagcag
     2101 acctccaggc tgttgcggcc ggggctcacc acaccctcct ggaacgcgaa ggagcataga
     2161 cccacctggc cccagacggc cgggtcgttg tacaggttga agggtccgtc gagtctctgc
     2221 tcgtcgtact gcgccagagt gtcggcgtgg tgatacgcgt acaggaccat cccgtcccag
     2281 tcctggagcg cggcgtacgc cgccaccatg gggagcgcgt cgcttcggaa ctcggtcccg
     2341 tacaggatgt tccattcccc cgcgatgaag ggtttgccgg ccacccggtc gtgattgagc
     2401 cgcgtcacca ggttgagctt gaacgggctg tcgaccacgg tcctgcgagg gtcgttctca
     2461 accatgagaa gatcgtggta gcgaggccga tcctgtctca cggtgtccgg gtggttccag
     2521 tacgcgttgt ccatggtgac atcggaatac cggtccaggc tgtagatgtc agcggctccc
     2581 tgggcgtggt tggtgacgtt gatggggcag cgcacgccca ggtccaggag gtggtcacgc
     2641 atctctcggc agaagtcgag ttgggtccgg gccaggaact cggtgtagtc cgcgtagcgc
     2701 gccgggtcct ccccaacgta catctgggtc ccttccaggt gcgggagcct tcgaacggtc
     2761 ccgagggacg ggtcctcatc gggttccagc cgatggacgc cggcggcgct ggtccaggcg
     2821 gcgtcgaggg ccgcgcggga gccatacttc gcaagaaggt accagccgaa cctgcggtcc
     2881 agctccttgc ggtaggaggg cagacccacc gggtcgcgcc cctcgtacca gaagatgccg
     2941 tcctcgttca tcacctggac cagtgccacc gccggatcct ccacgggccg caacgccgtg
     3001 tacggattac ggtgcgtcag gtaggcggtg gcgtactcct tgtggagctc catgagtcgg
     3061 cggttgtaga cggacgcttg cttgaagtac ttcatgaggt catccgggta gtccaggccg
     3121 tccgccggta ggaacccccg gtagcagaag aggtcgagct ggaggtagat gccgcgctgc
     3181 tccaggca
//
LOCUS       DASRZS010000563         3766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1093487, whole genome shotgun sequence.
ACCESSION   DASRZS010000563 DASRZS010000000
VERSION     DASRZS010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093487"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2580
                     /locus_tag="VFH83_13775"
     CDS             <1..2580
                     /locus_tag="VFH83_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487490.1"
                     /translation="EALAAYVLPLTDPTKAELDLEWPAFQTAGYGSLVITSVQEDTRK
                     LQQLVQTAQTQASDGDASLKRLAAFLAAPATDRSVSDLTALLSPFLAQVRTGASLLAL
                     ATSLTATGSSLRPNSQGDLPPYLQYLLWLLKGRPDRAEGIIAAVHGMYGMGTRLALEQ
                     VADSTTGLFTKAVAKVDSGDLAAASAAFAELSNRSSYVATVAGVASVQASAAVGWALS
                     PDDQSQAKRTLSAVALAQEQSAEIASFRSLIDLENGVASLPPAPSASPDALAKARASL
                     DGFGKSADEASRLARQRAETLSAKASPGVTVQPAVDAARSLSERLAAFSQSLHDVDLT
                     YALALARAQSSAFDDRLKDAVAAIPTGKDRLYGTVGGKAPAQDAFVPVQPTEASKIFD
                     SSTQSLVAVQTDMAAYRTRWKSDKPWVSSSSAIAALVKGLDSLDGRIASQKTELARLS
                     ALAADQHQQALAKRRVGDLDFNEGSRIIKSKQLDLFDNARTQLNDARDAYTDSLALEE
                     DARARSRLDPEIPDLLTQITDALYQRWLAEVDKLIDQGRAEFQKGEFLKAYNIVQTAE
                     SRWNEIHPDKTYSALETLLSQVTNALKLSWGRDLSPEDSRAPAVNAFLSQANASVSDA
                     EKLKKGDAQRVVLLNKATASVRSALAVAPVYRAAKLVQFRVRKDLAPNDRAFLDDARS
                     EIAAILKDYQARKGDRARIYYDLKDYTDIVPDYPGVKDALNQLEIELGFRIKPISPAD
                     VAAARDKYTQASTLYNADNALTYDPALQLLDTALNLNPGYTAARSLRMAILLKQGSPE
                     VSSISQGDLARYAKARQAYGTADYAGAYTILHDMIGANPKNGSYPPIAKLYLLAQQKL
                     GLK"
     gene            2577..>3766
                     /locus_tag="VFH83_13780"
     CDS             2577..>3766
                     /locus_tag="VFH83_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487491.1"
                     /translation="MKRILRPSLAARLCLAGAVLLAALTGAGAQGLFWDRPGALSAAG
                     VRFSSIAATQQSLVAAWEEIVPSAVRTEGAIYVSLAARGPQSDWRYRYRVVGPIRYSG
                     LDPGSEPLVYSMAVDKGRVLLAVATDEREISIYEGDDQLSFRQLTRLSTQVSVVTPSL
                     FVTSRGTLLLLVTQSSVAADATASTDSVSLLWSRSSDGRSWSALAPFVAGAEAGAGVQ
                     LQPDHASFHGKEVVVFQSQRSVTEGYQIYAKISPDGGETWQPAQAITSLPAFDQTVGG
                     TGYKAANFSNARPRLSPLGDKLAMGWERNLLGVASPVIYYCELNDKAEVSHPAEPVSG
                     SAPGIMAQPLRLADRDYLLYAESVSGSYRIVLSEHRGNAWVPSILGQTLSGSSRSPQG
                     IQLDG"
BASE COUNT          627 a         1343 c         1207 g          589 t
ORIGIN      
        1 gaggcccttg ccgcgtacgt ccttcccttg accgatccga ccaaggccga gctggacctg
       61 gagtggcccg cgtttcagac cgccggctat ggctcgctcg tgatcacctc ggtccaggag
      121 gacacccgga agctgcagca gctcgtgcag accgcccaga cccaggcttc ggacggcgac
      181 gcctcgctga agaggctcgc ggccttcctg gccgcaccgg ccacggaccg cagcgtttcc
      241 gacctgaccg cactgctatc gcccttcctg gcgcaggtcc ggacgggcgc ctccctgctc
      301 gcccttgcca catccctgac cgccacggga tccagcctgc ggcctaacag ccagggagac
      361 ctgccgccct atctccagta tctgctgtgg ctcctcaagg gacgcccgga ccgcgcggaa
      421 ggcatcatcg cggcggtgca tggaatgtac ggcatgggca cccggctggc ccttgagcag
      481 gtggcggaca gcacgaccgg ccttttcacc aaagcggtag ccaaggtgga ctccggagac
      541 cttgccgccg ccagcgcggc cttcgcggag ctatccaacc gctcatccta tgtggcgacc
      601 gtggccggag tcgcgtccgt gcaggcctcg gcagcggtcg gctgggcgct ctcgccggat
      661 gaccagtccc aggccaagcg gacgctctcc gccgtcgcgc tggcgcagga gcagtcagca
      721 gagatcgcat cctttcgatc tctgatcgat ctggagaacg gcgtggcctc cttgccgccc
      781 gcgccgagcg cgtcgccgga cgcgctggca aaggcccgag cctctctcga cggcttcggc
      841 aagagcgcgg acgaagcgtc ccgcctggcc cgacagcggg cggaaacgct ctccgccaaa
      901 gcttccccgg gggtcaccgt ccagcccgcg gtggatgccg ccaggtccct ctcggagcgg
      961 ctggcggcgt tctcccagag cctccacgac gtggatctca cctacgccct ggctctggcg
     1021 cgggctcagt catcggcgtt cgacgatcgg ctgaaggacg cggtcgcggc gattccgacg
     1081 ggcaaggacc gcctctacgg cacggtcgga ggcaaggctc cggcccagga tgcattcgtc
     1141 cccgtacagc cgacggaggc ctcgaagatc ttcgactcca gcacgcaatc cctggtcgcc
     1201 gttcagacgg acatggcggc ataccggacc cggtggaagt cggacaaacc gtgggtttcg
     1261 tcgagctccg ccattgccgc cctggtcaag gggttggaca gcctggacgg ccggattgcc
     1321 tcccagaaga cggagctggc ccggctgtcc gcgcttgccg cggaccagca ccagcaggcg
     1381 ctggccaaac ggcgggtcgg ggatctggac ttcaacgagg ggagtcggat catcaagtcc
     1441 aagcagctcg atctcttcga caacgcgcgg acgcagctca acgatgcccg agacgcgtat
     1501 acggactccc tggccctgga ggaggacgcc cgcgcccgtt cgcgcctgga tccggagatc
     1561 ccggaccttc tcacgcagat caccgacgcg ctctaccagc ggtggcttgc cgaggtcgac
     1621 aagctgatcg accagggccg ggccgagttc cagaaggggg agttcctcaa ggcatacaac
     1681 atcgtgcaga ccgccgaatc gcgctggaac gagattcacc cggacaagac gtactccgcc
     1741 ctggagaccc tgctctcgca ggtgaccaac gccctgaagc tgagctgggg gcgcgacctt
     1801 tctcccgagg actcgcgagc gccggccgtc aacgcgttcc tcagccaggc aaacgcgagc
     1861 gtttccgacg cggagaagct gaagaagggc gacgctcagc gcgtcgtgtt gctgaacaag
     1921 gcgaccgcca gcgtccgctc cgccctggcc gttgcgccgg tctatcgggc cgcgaagctt
     1981 gtgcagttcc gggtccgaaa ggacctggcg cccaacgacc gcgcgttcct ggacgacgcc
     2041 cggtcggaga tcgccgccat cctcaaggac taccaggcgc gcaagggaga ccgggcccgg
     2101 atctactacg acctcaagga ctacaccgat atcgtcccgg actatcccgg cgtcaaagac
     2161 gccttgaacc agctcgagat cgagctgggg ttccgcatca agccgatctc gccggcggat
     2221 gtcgcggcag cccgtgacaa gtacacccag gcttccacgc tctacaacgc ggacaacgcg
     2281 ctgacctacg atccggcgct ccagctcctg gataccgccc tcaacctgaa tcccgggtac
     2341 acggcggccc ggtcgctccg catggccatc ctcctgaagc aagggagccc ggaggtcagc
     2401 tcgatctctc aaggggacct ggcccggtac gcgaaggctc ggcaggccta tgggaccgcg
     2461 gactatgccg gcgcgtacac gatcctgcac gacatgatcg gcgccaaccc caagaacggg
     2521 agctatcccc ccatcgccaa gctctacctc ctggcgcagc agaagctggg tctcaaatga
     2581 agcggatcct tcgtccgtcg ctcgccgcca ggctctgcct cgcgggcgcc gttcttcttg
     2641 ccgcgctcac cggcgcgggc gcgcagggcc tgttctggga ccgaccgggc gcgctctccg
     2701 cggccggcgt ccgtttctcc agtatcgcgg cgacccagca gagcctggtc gcggcgtggg
     2761 aggagatcgt gccgtccgcg gtccggaccg agggagccat ctacgtgtcc ctggccgcgc
     2821 gcgggccgca gtcggactgg cggtaccgct accgggtcgt cggccccatc cgctactccg
     2881 gcctggatcc cggttcggag cccctggtct attccatggc cgtggacaag ggcagggtat
     2941 tgctcgcggt ggcgacggac gagcgggaga tctcgatcta cgaaggagac gaccagctct
     3001 cgttccgaca gctcacccgt ctctcgaccc aggtctcggt cgttacgccg agcttgttcg
     3061 tcacctcccg tggcaccctc ctgctcctgg tgacgcagtc ctccgtggcc gcggatgcca
     3121 cagcctccac cgactcggta agccttctct ggagccgttc ctcggacggc agatcctgga
     3181 gcgcccttgc ccccttcgtg gccggtgccg aggccggcgc cggagtgcag ctccagcccg
     3241 accacgcgtc gttccacggc aaggaggtgg tggtcttcca gtcgcagaga agcgtgaccg
     3301 aggggtacca gatctacgcg aagatcagtc cggacggggg tgagacctgg cagcccgccc
     3361 aggccatcac gagcctcccc gcgttcgatc agaccgtcgg cgggaccggc tacaaggccg
     3421 cgaacttcag caacgcccga cccaggctca gccccctcgg agacaagctc gccatgggct
     3481 gggaacgcaa cctgctgggg gtagcgagcc ccgtcatcta ctactgcgag ctcaacgaca
     3541 aggcggaggt ctctcatccg gcggagccgg tgtccggttc cgcgcccggg atcatggccc
     3601 agcccctgcg cctggcggat cgtgactatc tcctgtatgc cgagagcgtc tccgggtcgt
     3661 accggatcgt gctgagcgag caccgaggca acgcctgggt tcccagcatc cttgggcaga
     3721 cgctttcggg aagctcccgg tctccccagg gcatccagct cgacgg
//
LOCUS       DASRZS010000564         6449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5318359, whole genome shotgun sequence.
ACCESSION   DASRZS010000564 DASRZS010000000
VERSION     DASRZS010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5318359"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFH83_13785"
     CDS             <1..645
                     /locus_tag="VFH83_13785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET6487492.1"
                     /translation="FVGPRKGRESYLQLLTAPYDPGISLDYRAIPIVGLELAAFLSPD
                     TLATMGAYFQRSTVSFAGLAMSPWAGLALETAYALVLNLFVATAEESIFRGIVLEALG
                     PAWTALIFGAGHIPNILTYKTIDADSITQVALQSAFATLAGYYLSVVTVDKGYDLRTA
                     IAYHYWHNVVAMITGFLYDEGKAAASDATGSTSSGLSIGFSPADLELSVSIPLR"
     gene            complement(750..1709)
                     /locus_tag="VFH83_13790"
     CDS             complement(750..1709)
                     /locus_tag="VFH83_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6487493.1"
                     /translation="MRRLLLVLAVALAAAGMVVAAPKVKIAVSMPTADHGWMAGANWW
                     AQKAISDWKAKDPDIDFSLVTADTAQKQASDIEDLLVKKINGIVVFPYDDSLTTVVEK
                     AYKQGVYTVVLDRGTTKPVYDVWLRNDDQAYARQGMNWVASQLKGKGNVVLIEGIPSP
                     INTIRVDTAKAVAGQYPGIKILDSQPGNWNRQKALEVMQNYLQKYKQIDAVYTADDDM
                     LLGALQAYKESGRKDIKVWLGGGGSKELVKKIIDNSDPLIKADVTYPPDVVATGVSLA
                     VLGVRGRVFEGFYQKKLPVNIILNSEFIDSKNANDYYHPDAPF"
     gene            complement(1783..2751)
                     /locus_tag="VFH83_13795"
     CDS             complement(1783..2751)
                     /locus_tag="VFH83_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487494.1"
                     /translation="MAALSERLRRIDLQQYGSLFALIILFIASSLISPYFLQYQNVVN
                     ILNQVSYTGIIALGMTLVIVGGGIDLSVGSLAAFSGSLAVVVLNAVLPIAHSDGVAIA
                     AAALVSVIVGTAVGAANGALITWGRVAPFIVTLGTMALFRSLSLYIGNAGEFRSDSDA
                     FGDFGHIGFLYLPITVWVMVIMAAILSTVLYSTRYGRYVAAVGSNARVALYSAISVER
                     TRFFTYLIIGFCTGISAFLLASRFNAITTSNAGVGFELDAIAAVIIGGTAMTGGRGTV
                     WGTIAGAIILGMINNMLNLVGVSAYLQGTVKGIVVIGAVYIQRQRQ"
     gene            complement(2753..4261)
                     /locus_tag="VFH83_13800"
     CDS             complement(2753..4261)
                     /locus_tag="VFH83_13800"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6487495.1"
                     /translation="MSDVLVKTAGVGKSFSGVSVLRDIDLEIRRGEILGIIGENGAGK
                     STLMKILAGIHTPSAGTVWFDGRKVDLRRPVDARRVGISLVPQEFNLVSTLTVYDNVF
                     LGAERLGRWRLLDKRGMKRRTTELLAELGVALKPEARIEELSAAQKQMTEIAKALAFD
                     AKLLIMDEPTTVLTQHEIEALFRLMRRLRDGGMTIVYISHKLKEVKAICDRVVILRDG
                     AKIDDRPVAEITPLEMAERMVGRKLTQIFPDRKAPDPEVVLEVRHLSIPGVLKDIGFE
                     LHRGEILGLAGLVGAGRTEVAETLMGIRRPSTGEVRFRGRPLVVRRPSDAVRGGLSYL
                     SEDRQGSGILPTFTVAQNATLVSLRRYSRNPFGLLDGRAERESADRWRKLFSIRTPSV
                     DTRLENLSGGNQQKVSLSKSMDPRPRVLIADEPTRGVDVLAKTEIYGLLHTLVEEGIS
                     CLFISSELEEIIGMCHRVIVMKDGAVTGVLEGGQITEPEIMYRATGIREGTS"
     gene            4390..5832
                     /locus_tag="VFH83_13805"
     CDS             4390..5832
                     /locus_tag="VFH83_13805"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET6487496.1"
                     /translation="MSSKLTGLDPQIEASMKDWMVPGLALAVVEGDRLIHCAGYGSRD
                     PAQGLPMTPETRYRMASNTKAFTAMAVAMLVDEGKVAWDTPVRRYIPELRLFDPYATD
                     HLTPRDLLCHRSGLPAHDWSGWGGLDRASIPTRLAYLEPSCDIRTKLQYNNLMFMLAG
                     YLVERVSGAGYERFVTDRILKPLGMEHSGFSFGRAIQSGNFAECYWRKGDELVLYRHD
                     TASDPDEINPGAPAGGLVSTARDLVPWVVAHLNGGACGDTRIVSEACLTEMHTPQMID
                     NWPCRYPEMGHSSAALGWFVWTYRGQRLVLHGGAFGSQIVMMPSRRIGIIYLPTLGMY
                     SNLYEEICFTVFDRLLGLDPLPWHERLISEMGKQREKEAEEKAKGGCDRKAGTHPSRM
                     VEDFCGVYEHPACGKVTISSSGGSLFAKGAWEKLPLRHYHYDSFELLSDSGDPVVRLT
                     FHTDGTGVVSSFSGPMERSVKDVVFTRVSS"
     gene            5829..>6449
                     /locus_tag="VFH83_13810"
     CDS             5829..>6449
                     /locus_tag="VFH83_13810"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HET6487497.1"
                     /translation="MKEAVLLHIPHASTEIPAEVRAQILLSDEELRREVLTIVDRYTD
                     ELFDLPGATRVQSPWCRLVFDPERFRDDKDEPAAAFGRGAIYTRTVHGKPMRDPAPAA
                     RDALVRRFYDPYHALLESEVTQILAEHGECLIVDCHSFTSRPLPFEADQVSRRPDLCI
                     GTDPFHTPERLPGILQQLAEARGLSTRRNWPFGGTITPMRYYRKDGR"
BASE COUNT         1086 a         2179 c         2027 g         1157 t
ORIGIN      
        1 ttcgtcggtc cgcgaaaagg gagggaatcg tacctccagc tccttaccgc gccgtatgac
       61 ccgggcatct ctctggacta tcgcgccatt cccatcgtcg gcctggagct ggccgcgttc
      121 ctttcgccgg acaccctggc aaccatgggg gcctacttcc agcggagcac cgtgtccttt
      181 gccggactgg cgatgtcccc atgggccggc ctggccctgg agacggccta tgcgctcgtg
      241 ctgaacctgt ttgtcgccac ggcggaggag tcgatctttc gcggcattgt cctggaggcc
      301 ctgggtcccg cgtggacggc cctgatcttc ggcgccggcc acattcccaa catcctcacc
      361 tacaagacca tcgatgcgga cagcatcacc caggtcgcgc tccagtccgc gttcgccacg
      421 ttggccggct actacctgtc ggttgtgacg gtcgacaagg gctacgattt gcggaccgcc
      481 attgcctacc actactggca caacgtggtg gcgatgatca ccggcttcct ctacgacgag
      541 gggaaggcgg ccgcctctga cgccaccggt tcaacctcat ccggcctgtc gatcggcttc
      601 tctcctgccg acctggagct ctcagtttcg atcccgctcc gatgagagag aggccgggct
      661 tgtgcccggc ctcacgaact cgtcccacgg ggcgcgccta gaacggcgcg tcgaatgccg
      721 cgcctagaac ggcgcgtcgg atgccgcgcc tagaacggcg cgtcggggtg gtaatagtcg
      781 ttcgcgttct tgctgtcgat gaactccgag ttcaggatga tgttcaccgg cagtttcttc
      841 tggtagaagc cctcgaacac ccggccgcgc acgccgagca ccgccaggga gaccccggtg
      901 gccaccacgt cgggcggata ggtcacgtcc gccttgatga gcggatcgct gttgtcgatg
      961 atcttcttca cgagctcctt ggagccgccg ccgcccagcc agaccttgat atccttgcgg
     1021 ccgctctcct tgtacgcctg cagggcgccc aggagcatgt cgtcatcggc cgtatagacg
     1081 gcgtcgatct gcttgtactt ctggaggtag ttctgcatga cctccagggc cttctggcgg
     1141 ttccagttgc ccggctgact gtccaggatc ttgatgccgg ggtactggcc cgccaccgcc
     1201 tttgcggtat ccacccggat ggtgttgatg ggggagggga tcccctcgat cagcacgacg
     1261 ttgcccttgc ccttgagctg gctggcgacc cagttcatgc cctggcgggc gtaggcctgg
     1321 tcgtcgttgc gcagccacac gtcgtagacc ggcttcgtgg tgccgcggtc cagcacgacg
     1381 gtgtacacgc cctgcttgta cgccttctcg accaccgtgg tgagcgaatc gtcatagggg
     1441 aagaccacga tgccgttgat cttcttcacc agcaggtcct cgatgtccga cgcctgcttc
     1501 tgggccgtat ccgcggtgac cagcgagaag tcgatatcgg ggtccttggc cttccagtcg
     1561 ctgatggcct tctgggccca ccagttggcc ccggccatcc atccgtgatc cgccgtcggc
     1621 atggagacgg cgattttcac cttgggcgcc gcaacgacca tcccggccgc cgccagcgcc
     1681 actgcgagaa cgaggagcaa ccttctcacg acaatcctcc ctttcggaga tgtactcggg
     1741 cctcgacgag gcccatcggt attacgatcg atgatcccac ttctactgtc tctgtctctg
     1801 gatgtacacg gccccgatga ccacgatgcc cttcacggtg ccctggaggt acgcggagac
     1861 ccccaccagg ttgagcatgt tgttgatcat gcccaggatg atggcgccgg cgatggttcc
     1921 ccacacggtg ccccggcctc ccgtcatggc ggttcccccg atgatcaccg cggcgatggc
     1981 gtccagctcg aagccgacgc ccgcgttgga ggtggtgatg gcgttgaagc gcgaggccag
     2041 caggaacgcc gagatccccg tgcagaaccc gatgatgagg tacgtgaaga accgcgtgcg
     2101 ctccacgctg atcgcggagt acagcgccac ccgcgcgttc gagcccaccg cggccacgta
     2161 gcggccgtac cgggtcgagt acagcacggt gctcaggatc gccgccatga tcaccatgac
     2221 ccagacggtg atgggcagat acaggaagcc gatatggccg aagtcgccga aggcgtcgct
     2281 gtccgatcgg aactcccccg cgttgccgat gtacaggctc agcgagcgga agagcgccat
     2341 ggtgcccagc gtgacgatga acggcgccac ccgtccccag gtgatgaggg cgccgttggc
     2401 cgcacccacg gcggttccga cgatgacgga caccagggcc gccgcggcga tggcaacgcc
     2461 atccgaatgc gcgatcggga gcacggcgtt cagcactacc acggccaggg agccggagaa
     2521 cgccgccagg gagccgaccg agagatcgat cccgccgccg acgatcacca gggtcatccc
     2581 cagggcgatg atcccggtgt aggacacctg gttcaggatg ttgacgacat tctggtactg
     2641 caggaagtac gggctgatca gggacgacgc gatgaagagg atgatcagcg cgaagagcga
     2701 gccgtactgc tggaggtcga tgcgccgaag gcgttcggat agcgcggcca tgtcagctcg
     2761 tgccctcccg gatccccgtg gcccgataca tgatctcggg ctccgtgatc tgcccgcctt
     2821 ccagcactcc cgtcaccgcc ccgtctttca tgacaatcac ccgatggcac attccgatga
     2881 tctcctcgag ctccgatgag atgaagaggc aggagatccc ctcctccacg agcgtgtgca
     2941 ggaggccata gatctccgtc ttggccagga cgtcgacgcc gcgggtcggt tcgtccgcaa
     3001 tcagaacgcg cggccgcgga tccatgctct tggacaggga gaccttctgc tggttgccgc
     3061 cgctcaggtt ctccaggcgg gtgtccacgg aaggcgtgcg gatgctgaag agcttgcgcc
     3121 accggtccgc ggactcgcgt tccgcgcgtc cgtccaggag gccgaacggg ttgcggctgt
     3181 agcggcgaag gctcaccagg gtcgcgttct gggccaccgt gaaggtcgga aggatcccgc
     3241 tcccctggcg gtcctccgac agataggaaa gcccgccgcg caccgcgtcc gagggacgtc
     3301 ggaccaccag gggtctgccc cggaagcgca cctctccggt ggacggtcgc cggatcccca
     3361 tgagggtctc cgccacctcc gtgcgcccgg cccctaccag acccgccagc cccaggatct
     3421 ctccgcggtg cagctcgaac cctatatcct tcagcacgcc ggggatgctg agatgacgca
     3481 cctccagcac cacctccggg tccggtgcct tccgatccgg gaagatctgg gtgagctttc
     3541 ggcccaccat ccgctccgcc atctccagcg gcgtgatctc ggcgaccggc cggtcgtcga
     3601 tcttggctcc gtcccgcagg atcaccacgc ggtcgcagat ggccttcacc tccttgagct
     3661 tgtgggagat gtagacgatg gtcatcccgc catcccgcag ccggcgcatc aggcggaaga
     3721 gggcctcgat ctcgtgctgg gtgagcacgg tggtcggctc gtccatgatc aggagcttgg
     3781 cgtcgaaggc cagcgccttg gcgatctccg tcatctgctt ctgggcggcg ctcagctctt
     3841 cgatccgcgc ctccggtttc agcgccacgc ccagctccgc caggagctcc gtggtccgcc
     3901 gcttcatgcc ccgcttgtcc aggaggcgcc agcggcccag acgctcggcg cccaggaaga
     3961 cgttgtcgta gaccgtcagg gtgctgacga ggttgaactc ctgaggcacc agactgatcc
     4021 cgacgcggcg ggcgtccacc ggtctgcgca gatcgacctt cctgccgtca aaccacacgg
     4081 tccccgcgga cggcgtgtgg atcccggcca ggatcttcat cagggtggac ttcccggcgc
     4141 cattctcgcc gatgatcccc aggatctctc cgcgtcggat ctcgaggtcg atgtcccgaa
     4201 gcacggatac cccggagaac gatttcccga ccccggcggt cttcaccagc acgtcactca
     4261 cggcccaaag cctccgccgg ccagcgtctg atccatcgta gccatctcat ccagggcgga
     4321 aaccatagag ggccatggtg gatttgtcaa ggaagcccta tactggccta aacaaaaaag
     4381 gagggaagaa tgtccagcaa gctcaccggt ctcgatccgc agatcgaagc gtcgatgaaa
     4441 gactggatgg tgccgggact ggcgctcgcg gtcgtcgaag gggaccgcct catccactgc
     4501 gctggttacg ggtcacggga tccggcccaa ggccttccga tgacacccga gacgcgctac
     4561 cggatggcct ccaacaccaa agcattcacc gccatggcgg ttgccatgct ggtggatgag
     4621 ggcaaggttg cctgggacac gcccgtgcgc cgctacatcc ccgagcttcg cctctttgac
     4681 ccctacgcca cggaccacct gacgccccgg gacctgcttt gccaccgcag cggtcttccg
     4741 gcccatgact ggtccggttg ggggggactg gatcgagcga gcatcccgac acgcctcgcc
     4801 tatctggaac cgagctgcga cattcgcacg aagctccagt acaacaatct catgttcatg
     4861 ctcgccgggt acctggtgga gcgcgtgtcc ggcgcagggt acgagcggtt cgtcacggac
     4921 cggatcctga agcccctggg gatggagcat tcgggcttct ccttcggccg ggctatacaa
     4981 tccggaaact tcgccgagtg ttactggcgc aagggggatg agctggtcct gtaccgtcat
     5041 gacaccgcgt cggatccgga cgagatcaac cccggcgccc cagccggcgg cctggtctcc
     5101 acggcccggg acctggtccc ctgggtggtc gcgcatctga acggcggcgc ctgcggcgat
     5161 acccggatcg tctccgaagc gtgcctgacc gagatgcaca ccccgcagat gatcgacaac
     5221 tggccatgcc ggtatcccga gatgggtcac tccagcgcgg ccctggggtg gttcgtctgg
     5281 acgtaccgcg ggcaacgtct ggtgctgcac ggcggagcct tcgggagtca gatcgtgatg
     5341 atgccgtccc ggcggatcgg gatcatctat ctgcccaccc ttgggatgta ctccaacctc
     5401 tacgaggaga tctgcttcac cgtgttcgac cggctcctgg ggctcgatcc tctgccgtgg
     5461 catgaacggc tgatctcgga aatgggcaag cagcgggaaa aagaggcgga ggaaaaggcc
     5521 aagggaggat gcgaccgcaa ggccggtacc cacccgtccc gcatggtgga ggacttctgc
     5581 ggcgtctacg agcacccggc ctgcgggaag gtcacgatca gctcgagcgg agggtccctg
     5641 ttcgccaagg gcgcctggga gaagctgccc cttcgccact accactacga ttccttcgag
     5701 ctcctctccg actccgggga tccggttgtc cggctgacct tccacacgga cgggacgggc
     5761 gtcgtctcca gtttcagcgg gccgatggaa cgctcggtca aggacgtggt tttcacccgg
     5821 gtctcttcgt gaaggaggcg gtcctccttc acatccccca cgcgtccacc gagatccccg
     5881 cggaggtgcg cgcccagatc ctcctgagcg atgaggagct ccgcagggag gtgctcacca
     5941 tcgtcgaccg gtacacggac gagcttttcg atctacccgg tgccacgcgc gtccagagcc
     6001 cgtggtgccg gctggttttc gaccccgagc ggttccgcga cgacaaggac gagccggcgg
     6061 ccgcgttcgg gcggggagcc atctacacca ggaccgtgca cgggaagccg atgcgcgatc
     6121 cggcgcctgc ggcccgcgac gccctggtgc gccggttcta cgatccatac cacgctctcc
     6181 tggagagtga ggtcacgcag atcctggcgg agcacggaga gtgcctgatc gtggactgcc
     6241 actcgttcac gtcgcgaccg cttccgttcg aggcggacca ggtttcgcgg cggccggacc
     6301 tgtgtatcgg aacggatccc ttccatacac cggagcgcct gcccggaatc ctccagcagc
     6361 tcgcggaggc ccgcggcctg tccacccggc ggaactggcc gtttggcggc accatcactc
     6421 caatgcgcta ctaccgcaaa gatggtcgc
//
LOCUS       DASRZS010000565         5199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2223303, whole genome shotgun sequence.
ACCESSION   DASRZS010000565 DASRZS010000000
VERSION     DASRZS010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2223303"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /gene="proS"
                     /locus_tag="VFH83_13815"
     CDS             complement(<1..1109)
                     /gene="proS"
                     /locus_tag="VFH83_13815"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET6487498.1"
                     /translation="MAEKITPRATDYSQWYIDIVINAKLADYAPVKGCMVIRPRGYAL
                     WERMQQGLDGLFKQTGHVNAYFPLLIPESFIHKEAEHVEGFSPELAVVTHAGGEKLEE
                     PLVIRPTSETIIWSMYKKWIQSYRDLPILINQWCNVLRWEKRTRLFLRTTEFLWQEGH
                     TAHATAAEAEEETLRMLGVYRQFAEEFMAVPVLHGVKTDSEKFAGAVRTYCIEAMMQD
                     KKALQAGTSHNLGQNFAKAFDVTYQTNDKKVDYVWATSWGSSTRLIGALIMAHSDDKG
                     LVLPPRLAPTQVVIVPIFKNETREAVLTFARALRDALSPSVRVVLDEDDQATPGWKFS
                     EYELQGIPIRVEVGPRDMEKGQVVAVRRDTGEKIFL"
     gene            1225..1986
                     /locus_tag="VFH83_13820"
     CDS             1225..1986
                     /locus_tag="VFH83_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487499.1"
                     /translation="MEPYLELIDLAKSYGAREAVRGISLSIPKGQIVGLLGPNGAGKS
                     TTIAMLTGLLTPTSGEVRWEGRPIHDDLVRWRRSLGTVLEELSLFEHLTVREHLSLMG
                     RLAGLDATQTERRSEELLAFFQLRPFEDTLAIEASQGTRKKLAFALGLIHDPRVLLLD
                     EAFNGIDAITVRDLKRLIRELAAAEVTVILSSHVLDSAETLIDRCVIVDQGRIAYDRP
                     METVRASGRSLEDLYSSVLFGDKRLSPALSWMERS"
     gene            1989..3569
                     /locus_tag="VFH83_13825"
     CDS             1989..3569
                     /locus_tag="VFH83_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487500.1"
                     /translation="MGAVSLLTRFARLSRRRGTKLRDPRAADMMMYSWLVIVIFGRFA
                     YNCFLAAQQLALGHQEFGDAITRFSLAHFLLLSFLVPFLASFRSSDGLNRRRLALAAV
                     PARPLVVSELLTMASSPLNWAIALFAIPPVFPLLRLGHPVTSIIALVLGLVAAILLAR
                     AAASALALSSALQRLGGLLRYAFAAGLLVLVAANFDFQWKGGSIEALVFGKHLLLADP
                     TGAGLLAGLDRWSPSMWIYGGAPAACLGAVVLALALYLLSELAAYRKVGRGNAAQAGP
                     VLGGRLRLRPRGNGAPRARAPGGSVRSVLLRQELRSLRSLPTSALALLFGLACAVWLL
                     AAREASVNIALLGTVLATAVGFSYPSNVFGRDGQALRRYALAAPDWGEVFLAKNAAWL
                     AVCGLAFLPPLLADLLRVGPASALSLLLVCCVVLILCVLWGNLSSMLFPVLHASARPG
                     EPAPRAIFVNQIAPFAFWAVALGIDRSAGRFGSAGFDAAFAALLAVGLVAYRVLLRRI
                     ALQFDRDVEGVLERFRSG"
     gene            complement(3603..4652)
                     /locus_tag="VFH83_13830"
     CDS             complement(3603..4652)
                     /locus_tag="VFH83_13830"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487501.1"
                     /translation="MRNALSVARRIITQVAMDRRTLALALLAPLGVITLLWFVINGGL
                     TRPTIALLGSPPSQLRQALEDKAAIVDVATAQDGEDLVKAEKADALLDFSSTPPALTL
                     DGADPSVTAVVAQVVQRSSMQFLSEVPLLRGIMGNTQPSITLLHGRTDSTPFEFIAPV
                     MMGFVIFFFIFILSAISFLRERTSGTLERILATPATPMELVCGYILGFGFFAALETVL
                     IQVFTIWVLGIPATGSFLAILAMNLALCLVALSMGTFVSAFAATEFQVLQFIPIVIIP
                     QVLFSGILDLRQAPGWVRFLSRLFPLTYGGHALRELMLRGKPLGSLWVDLVVVLGFAG
                     LFVGLNAASVRRVKA"
     gene            complement(4645..>5199)
                     /locus_tag="VFH83_13835"
     CDS             complement(4645..>5199)
                     /locus_tag="VFH83_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487502.1"
                     /translation="CISRSVGYMAQDDALYPDLSGAQNLRFFAALYGLRGAASRAAMA
                     RVFALTRLQGEETKRVRHYSGGMRKRLSLAAALLHEPPILILDEPTIGIDPVLRAEFW
                     DEFRRLRAAGSTLLLSTHVMDEADRCDLLALIFDGRLLAYGTPAEIRASVGAGTTEDA
                     FLALRARSAEPQPSGTPSREAPRA"
BASE COUNT          800 a         1748 c         1736 g          915 t
ORIGIN      
        1 agaaagatct tctcccccgt gtctcgtcgg accgccacca cttgcccctt ctccatgtcc
       61 cgcggtccga cctccacgcg gatagggatg ccctgcagct cgtactcgga gaacttccaa
      121 ccgggggtgg cctgatcatc ctcgtccagg accacgcgga cggaggggga tagagcgtcc
      181 cgcagcgctc gggcgaacgt gagaaccgcc tcccgcgtct cgttcttgaa gatggggacg
      241 atgaccacct gcgtgggcgc caggcgaggc ggcagcacca gccccttgtc gtcggagtgc
      301 gccatgatga gcgcaccgat cagccgcgtc gacgagcccc aggaggtggc ccatacgtag
      361 tccaccttct tgtcgttggt ctgataggtg acatcgaatg ccttggcgaa gttctggccc
      421 aggttgtgcg aggtgcccgc ctgcaaggcc ttcttgtcct gcatcatcgc ctcgatgcag
      481 tacgtacgca cggcgccggc gaacttctcc gagtccgtct tgacgccgtg gaggaccggc
      541 acggccatga actcctccgc gaactgccgg tagacgccca gcatccgcag ggtctcctcc
      601 tccgcctccg cggcggtggc gtgcgccgtg tggccctcct gccagaggaa ctcggtggtg
      661 cggaggaaga gccgggtccg cttctcccag cgcaacacgt tgcaccactg gttgatcagg
      721 atggggagat cgcgatagga ctggatccac ttcttgtaca tgctccagat gatggtctcc
      781 gaggtggggc gaatcaccag cggttcttcc agcttctccc cgccggcatg ggtgaccacc
      841 gccagctccg gcgagaaccc ctccacgtgc tccgcctctt tgtggatgaa gctctcaggg
      901 atcaggagcg ggaagtacgc gttcacgtgg ccggtctgct tgaagaggcc gtccaggccc
      961 tgctgcatcc gctcccagag cgcgtagccg cgcggccgga tcaccatgca gcccttcacg
     1021 ggcgcgtagt cggccagctt cgcgttgatg acgatgtcga tataccactg ggagtagtcg
     1081 gtggcccggg gggttatctt ctctgccatt cccgctacct tgccagggcg ggatgcgggt
     1141 gtcaatggac cgcaccccgg ccgcacggcg gcgttcgttg acggtccgcc cgggaaaggc
     1201 caccatgcgg gcaatggagg agcgatggag ccctacctcg agctgataga cctcgccaag
     1261 agctacggcg ctcgggaggc cgtccgtggg atttcccttt cgatcccgaa ggggcagatc
     1321 gtgggtctcc tgggccccaa cggcgccggc aagtcgacca ccatcgcgat gctcaccggc
     1381 ctgctgactc ccaccagcgg tgaggtgcgg tgggaggggc ggcccatcca cgacgacctt
     1441 gtgcgttggc ggcgaagcct tgggacggtg ctggaggagc tttctctctt cgagcatctg
     1501 accgtgcggg agcacctgag cctgatgggg agactggccg gcctggacgc cacccagacg
     1561 gaacggcgct cggaggagct cctggcgttc ttccagctca ggccgttcga ggacaccctt
     1621 gccatcgagg cgtcgcaggg cacgcggaaa aagctggcgt ttgcgctcgg tctgatccac
     1681 gacccgcggg tgctgctcct ggacgaggca ttcaacggga tcgacgccat cacggtccgg
     1741 gacctgaagc ggctgatccg ggagctggcc gccgcggaag tcaccgtgat cctcagctct
     1801 cacgtcctgg actccgcgga aaccctgatc gatcgctgcg tcatcgtgga ccagggaagg
     1861 atcgcctacg atcggcccat ggagaccgtg cgcgcgtccg gacggtccct ggaagacctc
     1921 tactcctccg tgttgttcgg cgacaagcgc ctctcccccg ccctctcctg gatggagcgg
     1981 agctgagcgt gggagccgtc agcctcctca cccgtttcgc gcggctctcc cggcggcgcg
     2041 gcaccaagct tcgggacccg cgcgccgcgg acatgatgat gtactcgtgg ctggtgatcg
     2101 tgatcttcgg ccgcttcgcc tacaactgct tcctcgcggc ccagcagctt gccctggggc
     2161 accaggagtt cggcgatgcg atcacccggt tctccctggc gcactttctc cttctctcgt
     2221 tcctggtccc gttcctggcg tcgtttcgat cgtcggatgg cctgaaccgg cggaggctcg
     2281 cgctggcagc ggtaccggcc cggccgctgg tggtttccga gctgctcacc atggccagca
     2341 gccccctgaa ctgggccatc gcgctcttcg cgattccacc ggtgttcccc ctgctgaggc
     2401 tcggtcatcc ggtgacctcg atcatcgccc tggtccttgg gctcgtggca gccatccttc
     2461 tggcgcgcgc cgctgccagc gctcttgccc tctcatcggc cctccagcga cttggcggcc
     2521 tgctacggta cgcgtttgcg gccggcctcc tcgtgctggt ggccgccaat ttcgacttcc
     2581 aatggaaggg cgggagcatc gaggccctgg tgttcggcaa gcatctcctc cttgcggacc
     2641 cgaccggggc cgggctcctg gccggcctgg atcgctggtc tccctcgatg tggatctacg
     2701 gcggcgcgcc ggctgcgtgc ctgggagccg tggtcctggc cctcgcgctc tacctgctgt
     2761 cggagctggc ggcctaccgc aaggtgggga gaggaaacgc agcgcaggcg ggccccgttc
     2821 tgggagggcg actcaggttg cggcctcgcg ggaacggcgc gccccgtgcg cgagcgcccg
     2881 gcggatccgt ccgctccgta ctgctgcgcc aggagctgcg gtccctcagg tcgcttccca
     2941 cgtccgcgct cgccttgctg ttcggtctgg cgtgcgccgt gtggctcctc gccgccaggg
     3001 aagcctccgt gaatatcgcg ctcctgggga ccgtactcgc cacggccgtg ggattctcct
     3061 acccttccaa cgtgtttggg cgggatgggc aagcgcttcg acgatacgcg cttgccgcgc
     3121 ccgattgggg cgaggtcttt ctggcgaaga acgcggcatg gcttgccgtc tgtggattgg
     3181 cctttctgcc gccgctcctc gcggacctcc tccgcgttgg ccctgcctcc gcgctgtccc
     3241 ttctcctggt ctgctgcgtg gtcctgatcc tctgcgtcct gtgggggaac ctcagctcga
     3301 tgctctttcc ggtgcttcac gccagcgcgc gccctggcga gcccgctccc cgcgccatct
     3361 tcgtgaacca gatcgcgccc ttcgcgttct gggccgtggc cctggggatc gatcgctcgg
     3421 ccggaaggtt cgggtcggcg gggttcgacg ctgcattcgc cgcgcttctg gccgtcgggc
     3481 tcgttgccta tcgagtcctt ctgcggcgca tcgcgcttca gttcgatcgg gacgtagagg
     3541 gagtgctgga gagattccga agcgggtaga cctcagccca gcgcccaggc gctccgcccc
     3601 tcctaagctt tcacgcgcct cacgctggcc gcgttcagcc cgacaaacag tcccgcgaaa
     3661 cccagcacga cgaccaggtc cacccagagc gagccaaggg gctttccgcg cagcatcagc
     3721 tcgcgaagcg cgtggcctcc gtaggtcaga gggaaaaggc gggaaaggaa ccgcacccag
     3781 ccgggcgcct gccgcagatc caggatcccg gagaagagca cctgggggat gatcacgatc
     3841 ggaatgaact ggagcacctg gaactccgtt gccgcgaagg cggagacaaa ggtccccatg
     3901 ctcagggcca ccaggcaaag cgccaggttc atcgccagga tcgccaggaa cgagcccgtt
     3961 gccggaatcc cgagcaccca gatggtgaag acctggatca ggacggtttc caacgcggcg
     4021 aaaaaaccga agcccaggat gtagccgcac accagctcca tgggcgtcgc cggcgtggcc
     4081 aggatgcgtt ccagggtgcc ggaggtccgc tcccgcagga acgagatggc ggacaggatg
     4141 aaaatgaaga agaagatgac gaaccccatc atcacgggcg cgatgaactc gaaaggggtc
     4201 gagtccgttc taccgtggag gagcgtgatg ctgggttggg tgttgcccat gatcccgcgc
     4261 aggagcggca cctcggaaag aaactgcatc gacgatctct ggaccacctg cgccactacc
     4321 gcggtcaccg acgggtccgc gccgtcgagc gtgagcgcgg gaggggtcga gctgaagtcc
     4381 aggagcgcgt ccgctttttc cgctttgacc agatcctcgc cgtcctgggc agtggctaca
     4441 tcgacgatcg ccgccttgtc ctcgagtgcc tgccttagct gagacggcgg gctgcccagg
     4501 agcgcgatgg tgggtcgggt caggccgccg ttgatcacga accagagcaa cgtgatgacc
     4561 cccagcggag cgagcagagc cagggccagg gtgcgacggt ccatggcaac ctgcgtgatg
     4621 atcctgcgag ccacgctcag ggcattacgc acgcggcgcc tccctcgagg gcgtgcccga
     4681 cggttgcggt tcggcggagc gggcgcggag cgcaaggaac gcgtcttccg tggtgcccgc
     4741 gccgacggag gctcggatct ccgccggggt cccgtacgcc agcagccgcc cgtcgaagat
     4801 cagggccagg agatcgcagc ggtctgcctc gtccatcacg tgggtcgaga ggagcagcgt
     4861 ggagccggcg gctcgaagcc tgcggaactc gtcccagaac tcggcgcgca ggaccggatc
     4921 gatcccgatg gtgggctcgt ccaggatcag gatgggcggc tcgtggagca gcgcggccgc
     4981 cagagagagc cgcttgcgca tgccgccgga gtagtgcctc acgcgcttgg tctcctcccc
     5041 ctgaagtcgg gtcagcgcga acacccgggc catcgccgcg cgggaggcgg ccccccgcag
     5101 cccgtacagg gcggcgaaga agcgaaggtt ctgcgcgccg ctcaggtcag ggtacagggc
     5161 atcgtcctgg gccatgtacc cgacggagcg cgagatgca
//
LOCUS       DASRZS010000566         5676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1477834, whole genome shotgun sequence.
ACCESSION   DASRZS010000566 DASRZS010000000
VERSION     DASRZS010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477834"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFH83_13840"
     CDS             complement(<1..531)
                     /locus_tag="VFH83_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487503.1"
                     /translation="MNRDAVRSSVAQTGKAMRDTGIKRHAPTGTRYFTGYGDTVYDWE
                     LLFDTIVLGYFGSTDLTINGIRIFLNAQREDGFIPRRVRPAGIPQGMDAWGRAIHERE
                     EEEHCKPFLLQSALMVCRMRRDSSWLTVEDYRKLIRSVDHWMTAWDRDGNGLSEWASG
                     PHSGADTQFERIGPWRS"
     gene            618..1976
                     /gene="argA"
                     /locus_tag="VFH83_13845"
     CDS             618..1976
                     /gene="argA"
                     /locus_tag="VFH83_13845"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HET6487504.1"
                     /translation="MERSMLAEQVELIRQVFEYAHRFQGKTFVMKIDGALLEENYFPL
                     LVKDLVLLYRQGIRIILVPGAKKRIDEVLDRYHIPWKTEGGVRVSTPEALPFIKMAAF
                     DVSNQLMTQLAANNVDGVIGNWVKARGLGVLKGVDYQQTGVVERVDVDIIRKVLAEGL
                     IPIFPNIGWNALGTPYNISSNDLAAALAGALGAEKLFFIYGSQAAIGPGYRLPEGVAT
                     TQDGVVVYLSVPEAHALRNLNQDRSSEEMMEVIGLACRACAEGVNRVHVLNAEIEGVV
                     LKEIFSSRGGGMMIYSNEHENVRGMTPRDIPEVLRIMRPFMEREILLVRTEADLAARL
                     DEFVVYDVDGIIHACGALHPSKGMPGTAEIAAIAVDETYAGLGIGKRILTYLLAWAGR
                     LGLRQVHALTTQTADWFAQFGFREGSVTDLPEERRRSYNRQRNSRVLLLDLPAAPSAG
                     SP"
     gene            complement(2026..2523)
                     /locus_tag="VFH83_13850"
     CDS             complement(2026..2523)
                     /locus_tag="VFH83_13850"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6487505.1"
                     /translation="MTSREAFLETWKMESAATQRVLDAVPNAKLDYRPDPKSRTGLEL
                     AAHVAGHGPILNMLVETGSIKAGPMPAPKSLKDAVGAFAASLPKLEKLIKSTDDKTWD
                     TKMTGLYGPDGKAMMQMPLGNMVWFTFYDLVHHRGQLTTYLRAMGGKVPSVYGPSADD
                     PGRPA"
     gene            2620..3678
                     /locus_tag="VFH83_13855"
     CDS             2620..3678
                     /locus_tag="VFH83_13855"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6487506.1"
                     /translation="MSLSVSILALRDCTPLAPIGPMEVLAMANRLQGQKDGGGPPFRI
                     RLVSPGGNPVMASNGYPLHCHATIASVRSTDLVIIPAMDGDIQAQMDANRSCVPWIRR
                     MYGQGADVVSICTGAFLLAETGLLDGKSATTHWVAQDLFRRRYPKTHLVPEQIVVDNG
                     RICTSGGATSFLTLSIYLVEKYCGAETARLVSKMFLIDINKGPQTAYAIFGGQKAHSD
                     EEILKAQMVIEEEGSRELTVAEIATRVAMSPRNFIRRFKRATGNKPIEYLQRVRVEQA
                     KRALEAGSDAVSEIADRVGYHDAGSFRRIFRRVTGVTPAEYRSRYRYYGNAPTRTAAS
                     AVTPRPRPTPRRRSSPAS"
     gene            complement(3602..4066)
                     /locus_tag="VFH83_13860"
     CDS             complement(3602..4066)
                     /locus_tag="VFH83_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487507.1"
                     /translation="MSRMQIVVICLVVIVIWMGGRWLVNRGKGKGAGGRSASGDRTGA
                     RSVTAAGGRSAAASVDEINQWYQDAREQIAMMDQGELVRRERAWNALADAEKLAASEA
                     FLEERFGPKARRGFTRQEKLEIGKTARLTRPETSASSGSVGAAGSPQTPPSA"
     gene            complement(4071..>5676)
                     /locus_tag="VFH83_13865"
     CDS             complement(4071..>5676)
                     /locus_tag="VFH83_13865"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET6487508.1"
                     /translation="VSHDGEAIFGAQVLAAMEAQAFVEGDVDRLLDVGLSVIPKTSTI
                     YRMIGDIRGWNAADRDWMKTFRRIDERYGYDTYRGNCHIVPNHGVIVLGLLAGGGDFQ
                     RTLMIVNSCGLDTDCNSGNAGCLMGIKVGLAGINAGPDWRGPVADRLYVPTADGGRSV
                     TDAVRETYHIANTGLALAGRPAIHPKDGAQFHFELPGSVQGFRVDPAIESRGTAVLEN
                     VSGHSRTGSRSLAVRYHGLASGRTARVSSATFIPPEAVDMAGYGLMASPTLFPTQRLR
                     AEIQADGSNRAPVRCCLYVSVYGEKDRLELIRSPVTRLAPGRGEEIHWTVPSTGGRPI
                     AEVGAEISGDGGAEGAVYLDYLRWTGTPSLELGRTPDGGTMWKRAWVNGADRHESYSE
                     AFRIIQNEGRGLVMQGTRDWTDYRVSADITVCLARSAGIAARVQGMRRFYALVLDRGG
                     TARLIRSLEGDTVLGEIPFPWEQGSTHRFELEVQGTVLRASVDGKELPPATDDGSPLV
                     GGAIAFVCEEGWLSSNSIRVETVAEG"
BASE COUNT          948 a         1961 c         1847 g          920 t
ORIGIN      
        1 cgacctccac ggtccgatgc gctcgaactg ggtgtccgcc ccgctgtggg ggccactggc
       61 ccactcgctg aggccgttcc cgtcccggtc ccaggccgtc atccagtgat cgacggagcg
      121 gatgagcttg cgatagtcct ccaccgtgag ccatgaagag tcgcgtcgca tccggcagac
      181 catgagcgcg gactgcagga ggaacggctt gcagtgctcc tcctcctcac gctcgtgaat
      241 ggcgcggccc caggcatcca taccctgggg gatccccgcc ggacggacgc gccgaggaat
      301 gaagccatcc tcgcgctgcg cgttcaggaa gatgcggatg ccgttgatgg tgagatccgt
      361 ggagccgaag taccccagga cgatggtgtc gaagagcagc tcccagtcgt agaccgtgtc
      421 cccgtacccg gtgaagtagc gcgtgccggt aggcgcatgc cgcttgattc cggtatcccg
      481 catggctttc cccgtttgcg ccacgctcga gcgaacggcg tccctgttca tgcagccacg
      541 gtaacgggcg cggtcggggt gttcaagcgg cgccccccgc tcgctataat gcccgcggga
      601 gggaaccgcc cttggccatg gaaagaagca tgctcgcgga acaggtggag ctgatccggc
      661 aggtgttcga gtacgcccac cggttccagg gcaagacctt cgtgatgaag atcgacgggg
      721 ctttgctgga ggagaactac ttcccactcc tggtcaaaga cctggtcctc ctgtatcgcc
      781 agggcatccg gatcatcctc gtgccggggg cgaagaagcg catcgacgag gtcctggatc
      841 gataccacat cccctggaag accgagggag gggtacgggt ctccaccccg gaggcgctgc
      901 cgttcatcaa gatggccgcc ttcgacgttt ccaaccagct catgacgcag ctcgccgcaa
      961 acaacgtgga cggggtcata gggaactggg tcaaggcccg aggcctcggc gtgctgaagg
     1021 gcgtggacta ccagcagacg ggcgtggtag agcgcgtcga cgtggacatc atccgcaagg
     1081 tgctggcgga ggggctcatc cccatcttcc ccaacatcgg ctggaacgcg ttgggtacgc
     1141 cgtacaacat ctcctccaac gacctggccg cggcgctggc gggagcgctg ggggcggaga
     1201 agctcttctt catctatgga agccaggcgg ccatcggccc cgggtaccgg ctgccggagg
     1261 gcgtggccac gacgcaggac ggcgtggtgg tctacctcag cgtccccgag gcccatgcgc
     1321 tgcggaacct gaaccaggac cgaagctcgg aagagatgat ggaggtgatc ggcctggcct
     1381 gccgggcgtg cgcggagggt gtgaaccgcg tccacgtcct caacgccgag atcgagggcg
     1441 tggtgctgaa ggagatcttc tccagccgcg gaggcggcat gatgatctac tccaacgagc
     1501 acgagaatgt ccgcggcatg accccgcggg acatcccgga ggtcctgcgg atcatgcgcc
     1561 cgttcatgga gcgcgagatc ctcctggtcc gcacggaggc ggacctcgcg gcgcggctgg
     1621 acgagttcgt ggtctacgac gtggacggca tcatccacgc ctgcggcgcg ctgcaccctt
     1681 ccaagggtat gcccggcacg gcggagatcg cggccatcgc cgtggacgag acctacgcgg
     1741 gcctcgggat cggaaagcgg atcctgacct acctgctggc ctgggccgga cggctgggtc
     1801 tgcgccaggt acacgccctc accacccaga cggcggactg gttcgcccag ttcggcttcc
     1861 gggaagggag cgtgacggac ctgccggagg aacgccgccg cagctataat cgccagcgca
     1921 attcccgcgt gctcctcctg gacctgccgg ccgcgccttc cgcgggctcg ccctgaaggg
     1981 cggccggcat tcgccggccg ccacccgcgc aatcccgcgt gctcgctacg cgggcctccc
     2041 cgggtcgtcc gcggacggcc cgtagacgga cggcactttt ccacccatcg cccgaagata
     2101 cgttgtgagc tggccccgat ggtggaccag gtcgtagaac gtgaaccaga ccatgttccc
     2161 caacggcatc tgcatcatcg ccttgccgtc cggaccgtag agcccggtca tcttggtatc
     2221 ccaggtcttg tcgtccgtgc tcttgatgag cttctcgagc ttgggcaggg acgcggcaaa
     2281 cgcgccgacc gcatccttca gggacttggg cgcgggcatg ggcccggcct tgatgctgcc
     2341 cgtctccacg agcatgttca ggatcgggcc gtgtccggcg acatgggcgg cgagctccag
     2401 ccctgtccgg gacttcgggt ccggtcgata gtccagcttc gcattgggca ccgcgtccag
     2461 cacccgctgc gtggcagccg actccatctt ccaggtctcc agaaaagctt cacgactggt
     2521 catgcagtcc tcctggcctg gaatgtactg agaagcgacg acggggcgca gtggcgagtc
     2581 agactttccg cagaggcatt tgcgccatat cttgttccca tgagcctgag cgtttcgatc
     2641 ctggccctga gggactgcac gcccctcgca cccatcggac cgatggaggt cctggccatg
     2701 gccaaccggc tgcaggggca gaaggacggc ggcggtccgc cctttcggat ccggctcgtc
     2761 tcccccgggg ggaatcccgt gatggcctcc aacggatacc cgctgcactg ccacgccacg
     2821 atcgcctcgg tccgaagcac ggacctggtg atcatcccgg cgatggacgg ggatatccag
     2881 gcgcagatgg acgcgaaccg ttcctgcgtc ccctggatcc gccggatgta tgggcaaggg
     2941 gcggacgtgg tcagcatctg cacgggggcg ttcctccttg ccgagaccgg attgctggac
     3001 ggaaagagcg ccacaacgca ctgggtggcg caggacctct tccgccggcg ctaccccaag
     3061 acccacctgg taccggagca gatcgtggtg gacaacggca ggatctgcac cagcggcggg
     3121 gccacctcgt tcctgacctt gtccatctat ctcgtggaga agtactgcgg ggccgagacg
     3181 gcccggctgg tctccaagat gttcctcatc gacatcaaca agggccccca gacggcgtac
     3241 gcgatcttcg gcggccagaa ggcgcactcc gacgaggaga tcctgaaggc ccagatggtc
     3301 atcgaggagg aggggtcccg agagctcacc gttgcggaga tcgcgacgcg cgtcgccatg
     3361 agcccccgca acttcatccg ccggttcaag cgggcaacgg ggaacaagcc catcgagtac
     3421 ctgcagcggg tgcgcgtgga acaggccaaa cgcgcgctgg aggccggaag cgatgcggtt
     3481 tccgagatcg ccgacagggt cgggtaccat gacgccggca gcttccgcag gatcttccga
     3541 cgggtcacgg gcgtgacccc cgcggagtac cgaagccgct atcgatatta cggaaacgcc
     3601 cctacgcgga cggcggcgtc tgcggtgacc ccgcggcccc gaccgacccc gaggaggcgc
     3661 tcgtctccgg cctcgtgagc cgggccgtct tcccgatctc aagcttctcc tgccgggtga
     3721 atccacgccg cgccttgggt ccgaagcgct cctccaggaa cgcctcgctc gcggcgagct
     3781 tctccgcatc cgccagggcg ttccaggccc gctcccgtcg gaccagctcg ccctggtcca
     3841 tcatggcgat ctgctcccgc gcgtcctggt accactgatt gatctcatcg acgctggccg
     3901 cggcggagcg gccgccggcc gcggtgacgg accgggcacc ggttctgtcc cccgaggcgg
     3961 accggccacc ggctcccttg cctttgcccc ggttgacgag ccagcgtccc cccatccaga
     4021 tcacgatcac caccaggcag atgacgacga tctgcatccg cgacatgggc ctatccctct
     4081 gccaccgttt caacccgaat ggagttgctg ctgagccagc cttcctcgca gacaaaggcg
     4141 atcgccccgc cgaccagcgg gctgccgtca tccgtcgccg gcgggagctc cttcccgtcc
     4201 acggaggccc gaaggaccgt tccctgcacc tccagctcga accggtgggt gcttccctgc
     4261 tcccacggga acgggatctc gccgagcacc gtgtcccctt ccagggaccg gatcaggcgg
     4321 gcagtcccgc ctcgatccag gacgagcgcg tagaacctcc gcatcccctg gacccgcgcc
     4381 gcgattccgg cggagcgggc aaggcagacg gtgatgtcgg cgctcacccg gtagtccgtc
     4441 cagtcccgcg tgccctgcat caccaggcca cgaccctcgt tctggatgat gcggaacgct
     4501 tcggagtaag actcgtgccg atccgcgccg ttcacccagg cgcgcttcca catggtgccg
     4561 ccgtccggcg ttcgtcccag ctccaaagac ggggtcccgg tccatcggag gtagtccagg
     4621 tacacggccc cttcagcgcc gccatccccc gagatctccg cgcccacctc cgcgataggc
     4681 cgaccgccgg tagacggtac ggtccagtgg atctcttctc cacggcccgg tgccaggcgg
     4741 gtcaccggag aacggatcag ctccagccgg tccttctctc cgtagaccga gacgtacagg
     4801 cagcatcgga cgggcgcgcg gttggagccg tccgcctgga tctcagcgcg gagcctctgc
     4861 gtgggaaaca gcgtcggaga cgccatgagc ccatagcccg ccatgtccac ggcctcggga
     4921 ggtatgaagg tggcggagga gacccgcgcc gttcggcccg aggcaagccc gtgatagcga
     4981 accgccagag accgcgaacc ggtgcggcta tggcccgaca cgttctcaag cacggccgtg
     5041 ccccggctct cgatggccgg atccacccgg aaaccctgga ccgacccggg cagctcgaag
     5101 tggaactgag ccccgtcctt cggatggatc gcgggcctcc ccgccagagc cagaccggta
     5161 ttcgcgatgt ggtacgtctc ccggacggca tcggtgacgc tccgcccgcc gtccgccgtc
     5221 gggacgtaca accgatccgc caccggcccg cgccagtccg gtccggcgtt gatccccgcc
     5281 aggcccacct tgatccccat gagacaaccc gcgttgccgg agttgcagtc cgtgtcgagg
     5341 ccgcaggagt tgacgatcat cagggtgcgt tggaagtccc cgccgccggc cagcaggccg
     5401 aggacgatga cgccgtggtt gggcacgatg tggcagtttc cccggtacgt gtcgtagccg
     5461 tagcgctcgt cgatccggcg gaacgtcttc atccaatccc ggtccgccgc attccagccg
     5521 cggatgtcgc cgatcatccg gtagatcgta gaggtcttcg ggatgaccga gagtccgacg
     5581 tccaggaggc gatcgacgtc gccttccacg aacgcctgag cttccatcgc cgccaggacc
     5641 tgcgcgccga agatggcctc accgtcgtgg ctgacg
//
LOCUS       DASRZS010000567         3520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4930058, whole genome shotgun sequence.
ACCESSION   DASRZS010000567 DASRZS010000000
VERSION     DASRZS010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4930058"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="VFH83_13870"
     CDS             complement(<1..132)
                     /locus_tag="VFH83_13870"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET6487509.1"
                     /translation="MDAKRPLRRSRTNRMIGGVVGGLADYIGMDPVLARVLYVLIAVF
                     "
     gene            443..1576
                     /locus_tag="VFH83_13875"
     CDS             443..1576
                     /locus_tag="VFH83_13875"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6487510.1"
                     /translation="MEYRILGRSGLRVGEIGLGTEYLLNLTPDHVARVIHAAIDHGVN
                     YFDLFWPQPDFRDIMGAAFAGHREQVLLAAHLGSIMENGQPVIGRDPAVCEEYFQDFL
                     RRYHTDHVDVLFLFNSNTQEDYGSLLKPDGLLEVAYRHKQKGATRLIGFGGHNADTAR
                     QAVESGLFDVVIFPVNFTCRTVPGNRELHDACKREQVGLIAMKPYAGGRLLRDEAVLS
                     IEDFQMGRTQMAGAATRFEKRQAISPVQCLSYALSQPGVSTVVPGCKDLPQLDAALGF
                     LDATREERDVSHLLADFEHYPAGQCVYCNHCLPCPARIDIGKTLSLLDRAQEGLLPEI
                     RREFLAMSTQPSDCVECEDCTGRCPFGVDVVPRMRRAAELLAG"
     gene            complement(1606..2550)
                     /gene="metA"
                     /locus_tag="VFH83_13880"
     CDS             complement(1606..2550)
                     /gene="metA"
                     /locus_tag="VFH83_13880"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET6487511.1"
                     /translation="MPVRVPDDLPAKKILAEENIFVMSKERAIHQDIRPLRIAVLNLM
                     PTKIVTETQLLRLLGNTPLQVDITFIRMETHESKNTPTEHLDAFYESLDAVLHERFDG
                     LVITGAPVETLAFEQVDYWPELQRLMDWSRSNVWATMHVCWGAQAALYHHYGVPKYPL
                     SAKMFGLFPHRVLDPHEPLLRGFDELFMAPHSRHTEVRQGDIERVKGLKLLATSPEAG
                     VYLVASNDGRMVFITGHPEYDRLTLKAEYDRDIARGLRIDPPRNYFVDDNPASSPLVT
                     WKSHAHLLYANWLNYYVYQATPFDLDQLPRTDTPFQRE"
     gene            2683..3504
                     /locus_tag="VFH83_13885"
     CDS             2683..3504
                     /locus_tag="VFH83_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6487512.1"
                     /translation="MKRIVLIGVLMCAILAPLAAQSPGQRLKLATTTSTQDSGLLPIL
                     NPPFEKLTGISVDVIAVGSGNALALGRNGDVDVVLVHDPKAEAQFVAQGWGINRRDVM
                     HNDFVVLGPTDDPANVRAASTAAEAMSRISAAGASFISRGDNSGTNVKELDLWAKVGG
                     KPSGRWYKEAGQGMGAVLTMTANLRAYTLSDRGTWLAMQDKLPGLAIVCQGDPDLFNP
                     YGVIAVNPEKHPDVNYMAAMMYIAWVTSVQGQKIIREYTIAGQPLFTADAIHTSR"
BASE COUNT          616 a         1119 c         1173 g          612 t
ORIGIN      
        1 gaagacggcg atgagcacgt agaggacccg ggcaagaacg gggtccatcc cgatatagtc
       61 ggccaggcca ccgaccacac ccccgatcat ccggttcgtc ctggatcggc gcagcggcct
      121 cttcgcgtcc atagcataga ctcccttcga gggtagaaca ccagatcgcc gtagaacagt
      181 tcccggctcc cttgacaaaa cgccctgagc gggatatcta tcctaccgaa aatcctgaca
      241 tcatagcagc ctaccccact gttcaggcac ctttagcttt ctgcaccggg gcgggctgtt
      301 ttttttgccc ttcttggcgg cacctcgcca ggtcgccacg agcgcacgct ctttcctagg
      361 ggttgcgaga tactggagat tctgttaaaa aggacttgcg tctgaaaccg atgtatgata
      421 ctctcgcatt gaggcaaaaa agatggaata ccgaatcctg ggacgcagtg gcctgcgggt
      481 cggagagatc ggtcttggca ctgagtacct gctgaatctg actccagacc acgtggcccg
      541 ggtaatccac gcggccatcg accacggggt gaactacttc gatctgttct ggccgcagcc
      601 ggacttccgc gacatcatgg gggccgcttt tgccgggcat cgggaacagg tccttctggc
      661 cgcgcacctg ggctcgatca tggagaacgg gcagcccgtg atcggccggg atcccgcggt
      721 ctgtgaggag tacttccagg atttcctgag gcgctaccac acggatcacg tggacgtgct
      781 cttcctgttc aactcgaaca ctcaggagga ctacgggagc ctcctgaagc cggacggtct
      841 gctggaggtc gcctatcgcc acaagcagaa gggcgcgacg cggctcatcg ggttcggcgg
      901 ccacaacgcg gacactgccc gacaggccgt ggagagcggg ctcttcgacg tggtgatctt
      961 tcccgtgaac ttcacctgcc ggaccgtgcc gggaaaccgg gagctgcatg acgcctgcaa
     1021 gcgggagcag gtgggtctca tcgccatgaa gccctacgcc ggcggcaggc tccttcggga
     1081 tgaggccgtc ctcagcatcg aggatttcca gatgggtcgg acgcagatgg cgggcgcggc
     1141 cacccgcttc gagaaacgcc aggccatcag ccccgtccag tgtctctcct atgccctgtc
     1201 ccagccgggc gtctccaccg tcgtcccggg ctgcaaggac cttccgcagc tcgacgcggc
     1261 tctcggcttc ctggatgcga cccgggagga gcgcgacgtc tcccacctcc tcgcggactt
     1321 cgagcactac cccgcggggc agtgcgtcta ctgcaaccac tgcctcccgt gcccggcgcg
     1381 gatcgacatc ggcaagacgc tgtcgctcct ggaccgggcc caggaagggc tcctccccga
     1441 gatccgtcgg gagttcctgg cgatgtctac gcagccgtcc gattgcgtgg agtgtgagga
     1501 ctgcacgggc cgctgcccgt tcggcgtgga cgtggtcccg cggatgcggc gcgccgcgga
     1561 gctgctggcc ggctgagccg gccggggcgg ccgggagacg acccgttact ccctctggaa
     1621 cggcgtgtcg gtccggggaa gctggtccag gtcgaacggc gttgcctggt agacgtagta
     1681 gttcagccag ttcgcgtaca gcagatgggc gtgggacttc cacgtgacca gggggctcga
     1741 ggcgggattg tcgtccacga agtagtttcg gggcggatcg atccgcagcc ctctggcgat
     1801 gtcccgatcg tattccgcct tcagcgtgag ccggtcgtac tccgggtgac ccgtgatgaa
     1861 caccatgcgg ccgtcgttgg acgcaaccag gtagacgccc gcctccggtg aagtggccag
     1921 cagcttcagt cccttcaccc gctcgatgtc gccctggcgc acctccgtgt gccgggagtg
     1981 tggggccatg aacagctcgt cgaacccgcg caagagcggc tcgtgcggat ccaggacccg
     2041 gtgcgggaag agaccgaaca tcttggccga gagcgggtac ttggggacgc cgtagtggtg
     2101 gtagagcgcc gcctgcgcgc cccagcagac gtgcatcgtg gcccagacgt tgctgcggct
     2161 ccagtccatg agccgctgga gctcgggcca gtagtctacc tgctcgaacg ccagcgtctc
     2221 caccggcgcg ccggtgatca ccagcccgtc gaagcgttcg tgcaacaccg cgtcgagaga
     2281 ctcgtagaac gcgtccaggt gctccgtggg cgtgttcttg gactcgtggg tctccatccg
     2341 gatgaacgtg atgtccactt gcagcggcgt gttccccagg agcctcaaga gctgcgtctc
     2401 cgtcacgatc ttggtgggca tgaggttgag cacggcgatc cgcagcggac ggatgtcctg
     2461 gtggatggcc cgctccttcg acatcacgaa gatgttctct tccgcaagga tcttcttggc
     2521 cggaaggtcg tcgggtactc ggacgggcat gccgtactat agaggagcga ggccggggtg
     2581 acaagcgcgc ggtgaaggcg gatgacgggg cccgccgtga cgaccggccg tggctgcggc
     2641 tcgccttgac ggccgctccg gcccctccta tcctagccgg caatgaagag aatcgttctc
     2701 ataggcgtat tgatgtgcgc catcctcgcc ccgctggcgg cgcagagtcc gggacaacgg
     2761 ctcaagctgg cgaccaccac cagcacccag gacagcggcc tccttcccat cctcaaccct
     2821 ccgttcgaga agctgaccgg catctcggtg gacgtcattg cggtgggaag cggcaacgcg
     2881 ctggcgctcg gcaggaacgg cgacgtggac gtggtcctgg tgcatgaccc caaggcagag
     2941 gcgcagttcg tggcccaggg atgggggatc aatcgcaggg acgtgatgca caacgacttc
     3001 gtggtgctgg gtcccacgga cgatccggcg aacgtgcgcg cggcctccac ggcggcggag
     3061 gcgatgagcc ggatcagcgc ggccggcgcg tcgttcatct ctcgcgggga caactccggc
     3121 accaacgtca aggagctgga cctctgggcc aaggtgggcg gcaagccgag cgggcggtgg
     3181 tacaaggagg ccggccaggg gatgggggcc gtcctcacca tgacggccaa cctgagggcg
     3241 tacaccctga gtgaccgggg aacctggctg gccatgcagg acaagctgcc ggggctggca
     3301 atcgtctgcc agggggatcc ggacctgttc aacccctacg gcgtgatcgc cgtcaatcct
     3361 gagaagcatc cggacgtgaa ctacatggcg gccatgatgt acatcgcctg ggtcacctcc
     3421 gtgcaggggc agaagatcat ccgggagtac accatcgccg ggcagccgct gttcacggcg
     3481 gacgccatcc acaccagccg gtgaggggcg gaggcgatcg
//
LOCUS       DASRZS010000568         4364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_694025, whole genome shotgun sequence.
ACCESSION   DASRZS010000568 DASRZS010000000
VERSION     DASRZS010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694025"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFH83_13890"
     CDS             <1..447
                     /locus_tag="VFH83_13890"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6487513.1"
                     /translation="HDDFVWTAGAFMNPEIYRNVIIPRFAELWKPIHRAGKKVLFCSD
                     GNFMEFAQDIVEAGADGLIFEPCNDFGFMVERFGNSQCLVGSCVDCRDLTFNRWSKVE
                     ADIDRTFALLERCRGAIVAVGNHLPANIPDDVMTRYLAEVRARVRR"
     gene            complement(490..1371)
                     /locus_tag="VFH83_13895"
     CDS             complement(490..1371)
                     /locus_tag="VFH83_13895"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET6487514.1"
                     /translation="MNPRTPSPIPELLADYHCVCGEIPLWHPEERRVYWSDIPAGRLF
                     RYDPATGRHEEAFQGEPVGGMTLQKDGSLLLLMDRCQVCSWKDGALRPVVEKVPPGEE
                     KARWNDAIADPEGRVYAGTLPSEGHKGSLYRLDPDGTYTRLVEGFGCSNGMGFTGDLR
                     RFYHVDSTTRHVYLYDYQRATGAITNQRVFATLAASLGVPDGMTVDAKGFVWVAVWGG
                     SCVIRFSPNGEEDQRVYFTGKLVSCPTFGGEDYRDLYVTLAGGEKKDVNGPGAGALFR
                     VRPGVKGVPEFRSRIGL"
     gene            complement(1368..4208)
                     /locus_tag="VFH83_13900"
     CDS             complement(1368..4208)
                     /locus_tag="VFH83_13900"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HET6487515.1"
                     /translation="MNDMVAHLLALKSETQGYWAERIVSELQYATVLSRTLGRRHDTL
                     LRSTIESLASLFASTGGITRETVEETEAKLQSIAGDAKSLTVLCASHAHIDMNWMWRF
                     DETTIVALDTFRTILGLMTEYPGFIFSQSQASVYRIVEEHDSEMLKEIRKRVKEGRWE
                     VTASTWVEADKNIPNGESFARHALYARRYLSHLLDIPAESLLIDFEPDTFGHSRNVPE
                     ILSRAGVRWYYHCRGHDEHTLYRWQAPSGSQIIVYRDPFWYNGEVSPSMALAVPDFCK
                     KHGLTSMLRLYGVGDHGGGPTRRDIERIVDMDSWPVFPRFRFSPLKDFYAAAEANAEK
                     LPVVNKELNFVFTGCYTTQARIKMANRVSEASLVDAETYASLAALAAAAPYHTSQFAR
                     AWQMTLFNHFHDIVTGSGVVDTREYALGQFQRVLAIASSEASSALRHIAIHVDTSSLG
                     DPKEDARSTVSEGGGAGFGVADFRLSMVERGRGKIRVLHVFNPSPWQRSEAVEAIVWD
                     WDGDRSRVQVTDGTGAPIQHQVLPNEPLPNVGMVYWGHTHLRVLMNVTVPGCGYATYR
                     LCESLPSELPIPFPTDPRLERVQSTVLENEKIRAVFHPVTAALVSLVDKESGEEMVDG
                     NRDGGVFRLVREDDAKGMTAWIVGRWMSVQSAHQGVKLSAVSGGSDDLRQWLTYEMSI
                     ASSRLKATVSLDRGSAALCWSVECEWLELGRKGEGVPQLNFFLPLAAESKNFRYDVPF
                     GTADRAPVDMDLPANSWVLAVPKKAGHKSVLLVADQKHAFRCIDNSVSLTLLRSSYDP
                     DPYPELGLHRFSFSVEVTEGSSSARVARRAQVRAHALTCVSGTIHAGALPLSQGFLEA
                     EEGSAICSAVKMAEDSEAGGVVVRLYETDGRKGAATLRFFCAPSGAQAVDLNEKPLAD
                     GPAVSVKGSSVSVEMEPHAVTTILVRFQGCRE"
BASE COUNT          724 a         1458 c         1453 g          729 t
ORIGIN      
        1 cacgacgact tcgtgtggac cgccggggcg ttcatgaacc ccgagatcta tcgcaacgtc
       61 atcatcccgc gcttcgcgga gctgtggaag ccgatccacc gggccggcaa gaaggtgctc
      121 ttctgctccg acggcaactt catggagttc gcccaggaca tcgtggaggc cggcgcggac
      181 gggctcatct tcgagccgtg caacgacttc ggcttcatgg tggagcgctt cggcaacagc
      241 cagtgcctgg tgggaagctg cgtggactgc cgggacctga ccttcaaccg gtggtccaag
      301 gtggaggcag atatcgatcg gacgttcgcg ctcctggagc gctgccgcgg cgccatcgtg
      361 gccgtgggca accatctgcc ggccaacatc cccgatgacg tgatgacgcg ctacctggca
      421 gaggtccggg cgcgggtgcg gcgatagccg cctggggcgc gcgcggcccg gtggccgccg
      481 cggaccggcc tacagcccga tccgggaccg aaactccggc acgcccttca ctccgggccg
      541 cacccgaaag agagcgccgg ccccgggtcc gttgacgtcc tttttctccc cgccggccag
      601 ggtcacgtag aggtcccggt agtcttcgcc tccgaaggtg gggcaggaga cgagcttgcc
      661 ggtgaagtag actcgctgat cctcctctcc gttcggagag aagcggatca cgcagcttcc
      721 gccccatacc gccacccaga cgaatccctt tgcgtccacc gtcatgccat ccggcacccc
      781 aagggacgcg gccagggtcg cgaaaacgcg ctggttggtg atggctcccg tggcgcgctg
      841 gtagtcgtag agatagacgt gccgcgtcgt tgaatccacg tggtagaacc ggcgaaggtc
      901 gccggtgaag cccatgccgt tcgagcagcc gaagccctcg accagccgcg tgtaggtgcc
      961 gtccgggtcc agccggtaca ggcttccctt gtgcccttcg gaggggaggg tccccgcata
     1021 tacccggcct tcgggatccg cgatggcgtc gttccacctc gccttttcct caccgggagg
     1081 aaccttctcc accaccgggc gcagggcccc gtccttccac gagcaaacct ggcaccggtc
     1141 catcaagagg agcaggcttc cgtccttctg gagggtcatg ccgccgaccg gttctccctg
     1201 gaacgcctcc tcgtgacggc ccgtcgccgg gtcgtagcgg aagagtcgac ctgccgggat
     1261 gtccgaccag tagacgcgcc gctcctccgg gtgccagagc gggatctcgc cgcacacgca
     1321 gtggtagtcg gccaggagct ccgggatggg cgacggagtc cgcgggttca ctcccggcac
     1381 ccctggaacc ggaccaggat cgtggtcacg gcgtggggct ccatctccac gctcacggag
     1441 cttcccttca cgctgaccgc cggcccgtcg gcgagcggct tctcgttcag gtcgacggcc
     1501 tgggcaccgg acggcgcgca gaagaacctc agggttgccg cgcccttgcg gccgtcggtc
     1561 tcgtacaggc gaaccacgac gccgcccgcc tcggaatcct cggccatctt gaccgcggag
     1621 cagatcgcgc ttccctcttc cgcctccagg aacccctggg agagcgggag cgctccggcg
     1681 tggatcgtgc cggagacgca cgtgagcgcg tgcgcccgaa cctgggcgcg tcgcgcgacg
     1741 cgcgcggaag acgagccctc ggtcacctcc acggagaagc tgaagcggtg cagccccagc
     1801 tcggggtacg ggtccggatc gtacgagctt cgaaggaggg tcaacgacac ggagttgtcg
     1861 atgcagcgga acgcgtgctt ctggtccgcg acgaggagaa cgctcttgtg gccggccttc
     1921 ttggggacgg ccagcaccca gctattggcg ggcaggtcca tgtccaccgg cgcccgatcg
     1981 gcggtgccga aaggcacgtc atagcggaag ttcttgcttt ccgccgcgag ggggagaaag
     2041 aagttcaact ggggcactcc ctcgcccttg cggcccagct ccagccactc gcactccacg
     2101 gaccagcaga gcgcggcgga gccccggtcc agcgacaccg tcgccttgag ccgcgaggag
     2161 gcgatgctca tctcgtaggt gagccactgg cgcaggtcgt cgctgccgcc cgagaccgcg
     2221 gacagcttca cgccctggtg agcgctctgg acggacatcc agcggcccac gatccaggcg
     2281 gtcatgccct tggcatcgtc ctcgcggacc aggcggaaca ccccgccgtc gcggttgccg
     2341 tcgaccatct cctcaccgga ctccttgtcc accagggaca ccagcgccgc ggtaacggga
     2401 tggaacaccg cgcggatctt ctcgttctcc agcaccgtgc tctgcacgcg ctccagacga
     2461 gggtccgttg gaaacgggat gggcagctcg gacggcaggg attcgcagag tcggtaggtg
     2521 gcgtacccgc agccgggaac ggtcacgttc atcagcaccc gcaggtgggt gtgtccccag
     2581 tacaccatcc ccacgttcgg aagcggttcg ttgggcagca cctggtgctg gatcggcgcc
     2641 ccggtcccgt ccgtcacctg cacgcggctt cggtccccgt cccaatccca gacgatggcc
     2701 tccacggcct cgcttcgctg ccagggagag gggttgaata cgtgcaggac ccggatcttc
     2761 cctcggcctc gctccaccat ggagaggcgg aagtccgcca cgccgaagcc ggcccccccg
     2821 ccctccgaaa ccgtggagcg ggcgtcctcc ttcgggtccc ccagggagga agtgtcgacg
     2881 tggatggcga tgtggcgaag agcggaggac gcctccgagc tggcaatggc cagcacgcgc
     2941 tggaactgcc ccagggcgta ctcccgcgta tccacgacgc cggagccggt gacgatgtcg
     3001 tggaagtggt tgaacagcgt catctgccag gcgcgggcga actgcgaggt gtggtagggc
     3061 gcggccgcgg cgagcgccgc caggctggca taggtctccg cgtccaccag gctcgcctcg
     3121 ctcacccggt tggccatctt gatccgggcc tgcgtggtgt agcagcccgt gaagacgaag
     3181 ttgagctcct tgttgacgac cggaagcttt tcggcgttcg cctccgccgc tgcgtagaag
     3241 tccttgagcg gcgagaagcg gaaccgcggg aacacgggcc agctatccat gtcgacgatc
     3301 cgctcgatgt cgcgccgcgt gggcccgccc ccgtggtccc ccaccccgta gagccgcagc
     3361 atggaggtga ggccgtgctt cttgcagaag tccgggaccg ccagcgccat ggagggggag
     3421 acttccccgt tgtaccagaa gggatcccga tagacgatga tctgggagcc ggacggcgcc
     3481 tgccatcggt agagggtatg ctcgtcgtgg ccccgacagt gatagtacca gcgaacgccg
     3541 gcccgggaga ggatctcagg gacgttgcgg ctgtgaccga aggtgtccgg ctcgaagtcg
     3601 atgaggagac tctccgccgg gatgtccagg aggtgggaga gatagcgccg cgcgtacagg
     3661 gcgtgcctgg cgaagctctc cccgttcggg atgttcttgt ccgcctccac ccacgtcgaa
     3721 gcggtcacct cccaccggcc ttccttcacg cgctttcgga tctccttgag catctcggaa
     3781 tcgtgctcct ccacgattcg gtataccgag gcctgggact gggagaagat gaagccgggg
     3841 tactcggtca tcaggccgag gatggtccga aacgtgtcca gcgcaacgat cgtggtctcg
     3901 tcgaaccgcc acatccagtt catgtcgatg tgggcgtggg aggcacagag caccgtgaga
     3961 gacttggcgt ccccggcgat gctctgcagc ttggcctcgg tctcctccac ggtctcccgg
     4021 gtgatgccgc cggtggaggc gaagagggag gccagactct cgatggtgga gcgaaggagc
     4081 gtatcgtgcc gacgacccag ggtacgggac aggaccgtgg cgtattgcag ctcggagacg
     4141 atccgttccg cccagtaccc ttgggtctct gatttgaggg ctaagaggtg agcaaccata
     4201 tcattcatca cgaatcccct tttcgaaaaa ccggcctgag tgtggcgcct gcctttccgt
     4261 ttggtcaagg gtaaacgggc ccgttccgtc gaacgcctcg gccctcagta gccaacccgc
     4321 gacaaatgtt ctataacgcc agtatcgtgg atgtgcccaa gccg
//
LOCUS       DASRZS010000569         3424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5321301, whole genome shotgun sequence.
ACCESSION   DASRZS010000569 DASRZS010000000
VERSION     DASRZS010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5321301"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFH83_13905"
     CDS             <1..756
                     /locus_tag="VFH83_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487516.1"
                     /translation="WTTDEEHERLRAFVREGGTLIATGLTSLYRPDGSTTGDFGLADL
                     FGVSYTGRRARRFHYLSLDDGKRLVASHDSAPLVEARGAEVVGFINEPVFDPDDARYA
                     SIHSNPPGIQSRFAGLTVRSFGKGRCVYLSSPVLSLQQDAQQSFCAWLFATCAPAPMV
                     RETNAPAAVEITVLRSTTRNAYLVGFVNYQRELPNIPVRDLYAVLKLPGVGLPGPCTR
                     VSDGARLITGPSWDTLRVEVPVLETIEMIEVGF"
     gene            779..2704
                     /locus_tag="VFH83_13910"
     CDS             779..2704
                     /locus_tag="VFH83_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HET6487517.1"
                     /translation="MSRPPLLPPAFRPLPLGTVRPAGWLARQLRTQADGLSGHLDEFW
                     PDVKSSRWFGGDMEAWERAPYWLDGFVPLAVLLDDRTLLDKMHRFVGHILDHQPEDGW
                     LGPRTMVVAGGGKEAPRYDVWAQFLAIKMLVEYYDATRDERVPKAVERCLRKVDTHID
                     REPLMDWGQYRWFEALIGVHWLYERTGEPWVWDLGVKLHAQGFHWGDFFSQWPITEGT
                     PQGRWNFMGHVVNNAMAIKAHGLWWRQTGDPRDRAAAWDLMARLDRFHGQASGVFTGD
                     EVLAGRSPIQGTELCAVVEYAYSLEVLASLFGDPLLGDRLERIVFNAIPATFSPDMWS
                     HQYDQQSNQVECSVRDGRPWRANGPESNIFGLEPNYGCCTANLSQGWPKFAAHLWMET
                     PDTGLAAVAWAPCRLEATLGGAPVTVETETEYPFRDRLRVTVTAARPTRGPLLLRIPE
                     WASGATVQVGSGPREVAAAGTFHRVERAWSGRTEITLVFPMEASLWRGDRGSAAVVRG
                     PLVYALRVGEDWRRINQTRPGRELPHGDWEVYPTTPWNYGLALSDPFDPSELKFQEHP
                     VGDLPFSPAGAPVTAIARARKIPGWTAENGSAREVPQGPVQSGEPIEEITLIPYGCTN
                     LRITEFPLLAKKEGRAG"
     gene            2770..>3424
                     /locus_tag="VFH83_13915"
     CDS             2770..>3424
                     /locus_tag="VFH83_13915"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET6487518.1"
                     /translation="MAEKLRVAVVGATGMVGQRFVTLLAEHPWFQVAALAASERSAGK
                     CYTEAVGSRWMMSGSIPASIGSMVVKNASKMDEVTRDVDFLFCAVDMPKDETRNLEDC
                     YARMETPVVSTNSAHRMTPDVPMLLPEVNPEHLEVIARQRKRLGSKRGFITVKPNCSI
                     QSYVPALHPLLEFGVEKVFVCTYQAISGAGKTFKDWPEMVDNVIPYIGGEEEKSEMEP
                     "
BASE COUNT          548 a         1101 c         1213 g          562 t
ORIGIN      
        1 tggacgacgg acgaggagca tgagcggctc cgcgccttcg tgcgggaggg ggggaccctg
       61 atcgctacgg ggcttacctc gctctaccgg ccggacggct ccacgaccgg cgacttcggt
      121 ctggcggatc tcttcggcgt ctcctatacc ggcagacgag cccgtcggtt ccactacctg
      181 tccctggacg atggcaagcg cctggtggcc tcccacgatt cagcgcccct cgtggaggcg
      241 cgtggcgcgg aggtggtcgg attcatcaac gagccggtgt tcgatccgga tgacgcgcgc
      301 tacgcttcca tccacagtaa cccgccgggg atccagagcc gcttcgccgg gctgaccgtt
      361 cgctcgttcg gcaaggggcg ctgcgtctat ctctcctccc cggtgctatc gctccagcag
      421 gatgcccagc agagcttctg cgcgtggctc ttcgctacct gtgcgccggc gccgatggtc
      481 cgggagacca acgcaccggc cgccgtggag atcacggtgc tccgcagcac gacgcgcaac
      541 gcctatctgg tcggcttcgt caactaccag cgggagctcc cgaacatccc cgttcgggac
      601 ctgtacgcgg tgctgaagct gccgggggtg gggttgccgg gcccctgcac gcgcgtctcg
      661 gacggggcgc gcctgatcac gggtccttcg tgggacacgc ttcgggtcga ggtgccggtg
      721 ctcgaaacaa tcgagatgat cgaagtcgga ttctgagatc gatcctggag gcaatcgcat
      781 gagtcgaccg ccgcttctcc ctcccgcttt ccgcccgctt ccgctgggga ccgtaaggcc
      841 cgccgggtgg ctggcccggc agcttcggac gcaggccgac ggcctctcgg gtcacctgga
      901 tgagttctgg cccgacgtca agtccagccg ctggttcggc ggggacatgg aggcctggga
      961 gcgggctccc tactggctgg atggcttcgt gccgctggcc gtgctgctgg acgatcgtac
     1021 gcttctggac aagatgcacc ggttcgtcgg gcacatcctg gatcatcaac cggaggatgg
     1081 ctggctggga ccgaggacca tggttgtcgc gggcggcggc aaggaggcgc cacggtacga
     1141 cgtctgggcg cagttcctgg ccatcaagat gcttgtggag tactacgatg ccacccggga
     1201 cgagcgggta ccgaaggccg tcgagcggtg cctccgaaag gtcgacactc acatcgatcg
     1261 ggagcccctg atggactggg ggcagtatcg ctggtttgag gcgctcatcg gcgtgcactg
     1321 gctgtatgag cggacgggag agccctgggt gtgggacctg ggggtgaagc tccatgccca
     1381 ggggttccac tggggggact tcttcagcca gtggccgatc acggagggaa ctccccaagg
     1441 acgatggaac ttcatgggcc acgtcgtcaa caacgccatg gccatcaagg cgcacgggct
     1501 gtggtggcgc cagacggggg atccccggga ccgggccgcg gcctgggacc tgatggcccg
     1561 gctggatcgg ttccacggcc aggccagcgg ggtgttcacc ggggatgagg tcctggccgg
     1621 gcgcagtccc atccagggca cggagctgtg cgcggtcgta gagtacgcct actccctgga
     1681 agtcctggca tccctcttcg gcgatccgct gcttggcgat cggctggagc gtatcgtgtt
     1741 caacgcgatc ccggccacgt tctcaccgga catgtggtcc caccagtacg atcagcagtc
     1801 caaccaggtg gagtgcagcg tccgcgacgg caggccgtgg cgggcgaatg gaccggagtc
     1861 caatatcttc gggctggagc cgaactatgg ctgctgcacc gccaacctct cccaggggtg
     1921 gcccaagttc gcggctcacc tgtggatgga gaccccggat accgggctgg cggcggtagc
     1981 ctgggcgccc tgcaggctgg aggccaccct gggcggagcg ccggtgacgg ttgagacgga
     2041 gaccgagtac cccttccggg atcgcctccg cgtcacggtg accgccgccc gcccgacccg
     2101 gggcccactc ctcctgcgca ttcccgagtg ggcctcgggc gcgacggtgc aggtcggcag
     2161 cggcccgcgg gaggttgccg cggcgggcac gttccatcgc gtagaacggg catggtccgg
     2221 ccgcaccgag atcacgctgg tcttcccgat ggaggccagc ctgtggcgcg gggaccgcgg
     2281 ctccgccgcg gtcgtccgcg ggccgctcgt gtacgccctg cgcgtgggcg aggactggcg
     2341 gcgcatcaac cagaccaggc cgggccgaga gctgccccac ggcgactggg aggtctaccc
     2401 taccaccccg tggaactacg gcctcgcgct gagcgacccc ttcgacccga gcgagctgaa
     2461 gttccaggag catcccgtgg gggatctgcc attctccccc gccggggcgc ccgtcaccgc
     2521 catcgccaga gcgcggaaga tccccggttg gaccgctgag aacggctcgg cccgcgaggt
     2581 gccccagggg cccgtgcagt ccggggagcc catcgaggag atcacgctga tcccgtacgg
     2641 gtgcacgaac ctgcggatca ccgagttccc gctgctggcg aagaaggagg ggcgggctgg
     2701 gtgagggctt gagacgaacc agcgatcccg gtagagtggt tggaccctga agtcaggaga
     2761 ggttgggcaa tggcggagaa actgagagtc gcggtcgtcg gagcgaccgg catggtgggt
     2821 cagcggttcg tgaccctgct cgcggaacac ccgtggttcc aggtcgcggc gctggcggcc
     2881 agcgagcggt ccgcgggcaa gtgctacacg gaggcagtgg ggtcccgctg gatgatgagc
     2941 ggctcgatcc cggcgtccat cgggtcgatg gtcgtcaaga acgcctcgaa gatggacgag
     3001 gtcacgcgag acgtggactt cctcttctgt gccgtcgaca tgcccaagga cgagacccgc
     3061 aacctggaag attgctacgc ccgcatggag acgcccgtgg tctccaccaa ctccgcgcac
     3121 cgcatgactc cggacgttcc catgctgctg cccgaggtga atcccgagca cctggaggtg
     3181 atcgcccggc agcgaaagag gctcggctcg aagcgggggt tcatcaccgt gaagcccaac
     3241 tgctcgatcc agagctacgt tcctgccctc catcccctgc tggagttcgg agtggagaag
     3301 gtgtttgtgt gcacctacca ggccatctcg ggagccggca agacgttcaa ggactggccc
     3361 gagatggtgg ataacgtgat accctacatc ggcggcgagg aagagaagag cgagatggag
     3421 ccgc
//
LOCUS       DASRZS010000570         5124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4541258, whole genome shotgun sequence.
ACCESSION   DASRZS010000570 DASRZS010000000
VERSION     DASRZS010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4541258"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1045)
                     /locus_tag="VFH83_13920"
     CDS             complement(200..1045)
                     /locus_tag="VFH83_13920"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET6487519.1"
                     /translation="MLELPECVTIARQLDQALRDRTIAKVCGGNSPHKFAFIKPSVQE
                     LENALPGRRVSAVTASAKSMIMTLDPKGALSFHEMDARVLLTAPGGTLPSRYQFLLAF
                     DDGTVLTVTISLWGFIGLDDRSEAGMAEGSSSGPAGVPTERGIDPLSREFTVARLRKL
                     LADYEKKSASLKAFLVNRPCIKGFGNGYMQEILFAAGLRPTRAVGDLSTEEQARLHQA
                     IRSTLTSAVKLGGSEDERDVYGTPGRYQKMVGARVHRCKVCGADIEKKSFLGGATYWC
                     PRCQR"
     gene            1200..3395
                     /locus_tag="VFH83_13925"
     CDS             1200..3395
                     /locus_tag="VFH83_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET6487520.1"
                     /translation="MTIVVLLAGCLFLLASLVARLAGSSGGFGAAGGFLLESFSWAGF
                     FVPVYLLAAVVLLRARSFRRRSALLLIFSILPFLTLSLLFQVLGRSDSTLPQILVDAF
                     GLVPCALLLFLLLALETIFMLTLPARPTARSEGRRIAARPLAIPLIEPLEENIRLAPA
                     DVEPEPAPAAPVIVTEPPMDVTEPPMDVTEPPMDVTEPPMATDVEASPAVAEQAVPPV
                     ATVEESLPEPEPEAQPAPAPAADESPEPSQVQNELPFPQPSAKPKAYGIPVHDVLKNR
                     GDNDYWVIDEETKRSSKLLTDALKEFDIEAEVTGIRKGPVITLFEVLPAPGVRVSKIA
                     NLADNIALRLAAPSVRIVAPIPGRHAVGIEVPNRVRAIVAFKEMVSLDQMQKHAAEIP
                     IALGKDILGEAQVIDLIQTPHLLIGGATNSGKSVLVNTIICSILFRRSPEEVRLFLID
                     PKIVELKLYNGCPHLLTPVITEPRKAFQALQYCISEMERRYTLLDALGVRDIRAFNKK
                     AKKERVQRLPYIVVVIDEFADLMATTGKELESTLARLAAMARAIGIHMVLATQRPSID
                     VITGLIKANIPSRIAFMVANKFDSRIIIDTVGAEKLLGRGDMLFSAAWNPFPVRIQGA
                     FVDDEEIDAVTEYVKKMGPPQYIDDEVFFDDEETGEFQGEPGNDPMYNKALEIVIGQG
                     KASASYLQRRLKVGYNRAARLVEEMEERGIVGPAQGSKPREVLHVPSKG"
     gene            3460..4452
                     /locus_tag="VFH83_13930"
     CDS             3460..4452
                     /locus_tag="VFH83_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HET6487521.1"
                     /translation="MLQKRGSSSDKAGDKPKRETRPRKRKSKVTLQGLTAPEAVQGPP
                     PQEIESLCDAIRQRGGGVVGAYRDPIGGHWQIMASLPVDQVEPTPFQRDLSESHLARL
                     ADVVGRMGRFLDPIIAVRSAEGGFWTPNGNHRLGALKKIGARSILAVVVPELSVAYQI
                     LAMNTEKGHNLREKALEVVRMARSLAALEPRAEKDYALEFEEPDYLTLGLAYEQRGRL
                     SGGAYHPILKRVEEFLDAPLADAIQTRAARAEKLLVLDDTVAETVAALKEHGLTSPYL
                     KAFVVARINPLRFRKGAMEPDAALDAMLAAAQKFDAAKIKVSDLASAPPGPAEE"
     gene            4567..>5124
                     /locus_tag="VFH83_13935"
     CDS             4567..>5124
                     /locus_tag="VFH83_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6487522.1"
                     /translation="MSGYIMLLPEILVFTVFLVYPLIRGIFNSFYSFKGPTPVWTGLA
                     NFGLVFHDDLFWIALKNTTVYTVLVVPGGILVALATSWLIYPLRDRAQTFYKGAFYLP
                     GVVSGVVVALSWKWIFEPTSGLFNYGLSLVHLGPVPWLTSPNTALYSLILMVIMGGQG
                     ASIVIILANMMAISQSIFESARLDGC"
BASE COUNT          866 a         1731 c         1662 g          865 t
ORIGIN      
        1 gcgcaggagc gcgtcgggca cctgccggtc ctgcatggcg acgattgcct gaaacaccga
       61 cgtggcctgt tccacggtcc ggacctgggc ggcctgagcc caggccgggt cggtcagaca
      121 ggacaccagg accccgatgg caacccaacg tagagacgat gcgcgaacac tcacggcctg
      181 ctcctcggat ggatactacc tatctctgac accgggggca ccagtacgtg gcacctccca
      241 ggaaagattt cttttcgatg tcggcgccgc agaccttgca ccggtggact ctggccccga
      301 ccatcttctg gtaccgtccc ggggttccgt acacgtcccg ctcgtcctcg cttcctccga
      361 gcttcacggc gctggtcagc gtggaccgga tggcctgatg gagtcgggcc tgctcctcgg
      421 tggagaggtc accgacggcc cgggtgggac ggagacccgc ggcgaacagg atctcctgca
      481 tgtacccgtt tccgaaccct ttgatgcacg ggcgattgac gagaaaggcc ttgagcgagg
      541 cgctcttctt ctcgtagtcc gccagcagct ttcgcagccg ggccacggtg aactcgcggg
      601 agagcggatc gatgccccgt tccgtcggca ccccggccgg gcccgaggat gatccctccg
      661 ccatacccgc ttccgatcga tcatccaggc cgatgaagcc ccacagggat atggtgaccg
      721 tcaggacggt gccgtcatcg aaggcgagaa gaaactggta gcgagacggc agcgttcccc
      781 ccggggccgt gagcaggacc cgggcgtcca tctcgtggaa cgagagggct cccttggggt
      841 ccagggtcat gatcatggac ttggcgcttg ccgtgaccgc agaaaccctc cgtccgggca
      901 gcgcattctc cagctcctgg accgatggct tgatgaaggc gaacttgtgc gggctgttgc
      961 ccccgcagac cttggcgatg gtcctgtccc gcagcgcctg gtccaactgg cgggcaatcg
     1021 tcacacattc cggcaattcc aacattttcg tcctccccgt gctcgatctg ctccctagag
     1081 tagcgccggg ccggcctcca ccgcaactgc cgctacaacc gggacccagc cccgccgata
     1141 tctctaagag gggggttcgc gtggccaatt ccagcaggag acgctctcct gccttcctgg
     1201 tgacgattgt cgttctgctc gcggggtgcc tgtttctcct ggcatccctg gtcgctcggc
     1261 tggccgggtc ttccggcggc ttcggcgctg ccggcggatt ccttctggag agcttctcct
     1321 gggccggctt cttcgtgccc gtctacctgt tggccgccgt ggtgctgctg cgagcccgaa
     1381 gcttccgccg gcgatcggcg ctgctcctga tcttctcgat cctcccgttc ctgaccctga
     1441 gcctcctgtt ccaggtcctg gggcggtcgg actcgacgct tccgcagatc ctggtcgatg
     1501 cctttgggct ggtcccctgc gccctgcttc tcttccttct cctggcgctg gagaccatct
     1561 tcatgctcac ccttccagcg cgcccgacag cccggagcga agggagacgc atcgccgcca
     1621 gaccgctggc cattcccctg atcgagcctc tcgaggagaa catccggctc gcgcccgctg
     1681 atgtcgagcc cgaaccggct ccggcagcgc cggtgatcgt gaccgagccg ccgatggacg
     1741 tcaccgagcc gccgatggac gtcaccgagc cgccgatgga cgtcaccgag ccgccgatgg
     1801 ctacggacgt cgaggcgtcg cctgcggtcg cggagcaggc ggttccccct gtggcgaccg
     1861 tggaagagtc cctgccggag cccgaaccgg aggcgcaacc ggcaccggca ccggcggcgg
     1921 acgagagccc tgagccatcc caggtccaga acgagctgcc ttttccgcag ccctcggcca
     1981 agccgaaggc gtacggcatc ccggtgcacg acgtgctcaa gaaccgcggg gacaacgact
     2041 actgggtcat cgacgaggag accaagcgct cctccaagct cctgaccgat gccctcaagg
     2101 agttcgacat cgaggcggag gtgaccggca tccgcaaggg ccccgtcatc acgctcttcg
     2161 aggtcctgcc ggcgccgggg gtccgggtct ccaagatcgc caacctggcg gacaacatcg
     2221 ccctgaggct ggcggctccc agcgtgcgca tcgtggcccc catcccgggc cggcacgcgg
     2281 tcgggatcga ggtgcccaac cgcgtccgcg ccatcgtcgc gttcaaggag atggtctccc
     2341 tggaccagat gcagaagcac gcggcggaga ttcccattgc cttgggaaag gacatcctcg
     2401 gggaagctca ggtcatcgac ctgatccaga cgccgcacct cctgatcggc ggagccacca
     2461 actccggcaa gagcgtgctg gtcaacacca tcatctgctc catcctcttc cgacgatcgc
     2521 cggaggaggt gaggctcttc ctcatcgatc ccaagatcgt ggagctcaag ctgtacaacg
     2581 gctgccccca cctgctcacg ccggtgatca cggagcccag gaaagcgttc caggcgctgc
     2641 agtactgcat ctcggagatg gagcgccgct acacgctgct ggacgcgctg ggggtgcggg
     2701 acatccgcgc cttcaacaag aaggccaaga aggagcgcgt gcagcggctg ccgtatatcg
     2761 tggtggtgat cgacgagttc gccgacctca tggcgaccac cggcaaggag ctggagtcca
     2821 ccctggcccg gctcgccgca atggcgcgcg ccatcggcat ccacatggtg ctggccacgc
     2881 agcgaccgtc catcgacgtg atcacgggtc tcatcaaggc caacatccct tcccgcatcg
     2941 cgttcatggt ggccaacaag ttcgactcgc ggatcatcat cgacacggtg ggagcggaga
     3001 agctgctggg ccggggagac atgctgttct ccgccgcgtg gaacccgttc cccgtccgca
     3061 tccagggggc gttcgtggac gacgaggaga tcgacgcggt caccgagtac gtcaagaaga
     3121 tggggccgcc ccagtacatt gacgacgagg tcttcttcga cgacgaggag accggggagt
     3181 tccagggcga accgggaaac gacccgatgt acaacaaggc cctggagatc gtgatcgggc
     3241 aagggaaagc gtcggcatcc tatctgcagc ggcggctcaa ggtggggtac aaccgcgccg
     3301 cccgcctggt ggaagagatg gaggagcgcg gcatcgtggg cccggcccag gggagcaagc
     3361 cgcgcgaggt cctccacgtg ccgtcgaaag gctgaccggc ggcgcggggc ccctcgctcc
     3421 cagcggggcc ccgtgatacc atccggacgg agaaggtcca tgctgcagaa acgcggatcg
     3481 tcgagcgaca aggcgggtga caagcccaag cgcgagacca ggcctcgcaa gcgcaagagc
     3541 aaggtgaccc tccaggggct gaccgcgccg gaggccgtcc aaggcccgcc tccgcaggag
     3601 atcgagagcc tgtgcgacgc catccgccag cggggcggcg gggtggtcgg cgcctaccgc
     3661 gatcccatcg gcggccactg gcagatcatg gccagccttc ccgtggacca ggtggagccg
     3721 actcccttcc agcgcgacct ctcggagagc catctggcca ggctcgcgga cgtggtgggc
     3781 cggatgggcc ggttcctgga tcccatcatc gccgttcgaa gcgccgaggg cggattctgg
     3841 acgcccaacg gaaaccatcg gctgggggcc ctcaagaaga tcggggcccg atccatcctg
     3901 gcggtcgtgg tcccggagct ctcggtcgcc taccagatcc tggcgatgaa cacggagaag
     3961 gggcacaacc tccgggagaa ggcgctggag gtggtgcgga tggcgcggtc cctggcggct
     4021 ctggaaccca gggcggagaa ggactacgcc ctcgagttcg aggagccgga ctacctcacg
     4081 ctgggactcg cctatgagca gcgcggtcga ttgagcggcg gcgcctatca ccccatcctc
     4141 aagcgggtgg aggagttcct ggacgcgccg cttgcggacg ccatccagac gcgcgccgcg
     4201 cgagcggaga agctcctggt cctggacgac acggtggcgg agaccgtcgc ggcgttgaag
     4261 gagcacgggc tcaccagccc ctatctcaag gcgttcgtgg tggcgcggat caatccgctt
     4321 cggtttcgaa aaggcgcgat ggagccggac gccgccctgg acgccatgct ggccgccgca
     4381 cagaagttcg acgccgccaa gatcaaggtg agcgacctgg ccagcgcccc tccgggtccc
     4441 gcggaggagt aggacccgcc gaggatgtcc gatcgtgggg ttgacacccc cgagcccgcc
     4501 ccctaccgtt gacacgtggt gagagcgcgg gaggcggttc acctttccga acgggcgaag
     4561 aggaacttgt ctgggtacat catgctcctc cccgagatcc tcgtattcac ggtcttcctg
     4621 gtctatccgc tgatccgagg tatcttcaac agcttctaca gcttcaaggg tccgactccc
     4681 gtctggacgg ggctggccaa cttcggcctg gtcttccacg acgacctttt ctggatcgcg
     4741 ctcaagaaca cgacggtcta caccgtcctg gtggtgccgg gcggtatcct ggtggctctg
     4801 gccacgtcct ggctcatcta tccgctcagg gaccgggccc agacgttcta caaaggggcg
     4861 ttctatctgc cgggcgtggt ctccggcgtc gtggtcgccc tctcgtggaa gtggatcttc
     4921 gagccgacct ccggcctctt caactacggg ctctcgctgg tccacctcgg tccggtccca
     4981 tggctgacca gtccgaacac ggcgctgtat tccctgatcc tcatggtgat catgggcggg
     5041 cagggcgctt ccatcgtcat catcctggcc aacatgatgg ccatctccca gagcatcttc
     5101 gagagcgccc ggctggacgg gtgc
//
LOCUS       DASRZS010000571         3381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_316228, whole genome shotgun sequence.
ACCESSION   DASRZS010000571 DASRZS010000000
VERSION     DASRZS010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316228"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /gene="phnD"
                     /locus_tag="VFH83_13940"
     CDS             complement(<1..587)
                     /gene="phnD"
                     /locus_tag="VFH83_13940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HET6487523.1"
                     /translation="MSRRIAFLCLAALAVTAMGGWAADPLGSETNPFIWAYVPSGDTA
                     QVSAGAQSVADLLHAKTGLFFKTFVATDYVGVIEALKASPPRAQITSLATAAYILAAD
                     SGVSQAALVSVRNGAAFYQGEIITRPDTGIKTLADLKGHSFARVDPLSASGWIIPSLM
                     IRAAGLIPEVDMKVLDAGSHPGVATAVYSGQADAGA"
     gene            697..1188
                     /locus_tag="VFH83_13945"
     CDS             697..1188
                     /locus_tag="VFH83_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487524.1"
                     /translation="MRKAVCLLLLVACPFLAAAQDETRAGPAYTVQSPGKYLHFLAGM
                     SLGMLGAGLAEYALQPTYPSQYPLVLPSVAISVALPAGIAKELLDATGFGDARLTDVL
                     ITTAGGLLASMMVGYAEALYPANSRGKSNSVALLLSTSALLAVPVAIGFAREVRNNLE
                     KRR"
     gene            1185..>3381
                     /locus_tag="VFH83_13950"
     CDS             1185..>3381
                     /locus_tag="VFH83_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HET6487525.1"
                     /translation="MRQASRRSTLGALFLAVAVSTAAAPRDLSSAPTLYVVGYAHLDT
                     EWRWEYPQVIREFLPNTLRRNFALLDRYPHYVFNFTGSNRYQMIQEYFPADFARLKGY
                     VGAGRWFPAGSSVEESDVNVPSAESILRQVLYGNEFFRRELGTQSDEYMLPDCFGFPA
                     TLPSLLAHAGLRGFSTQKLVWGSAAPVGGIGSPEQTPRGIPFNVGTWIGPDGRGVIAA
                     LNPGDYTSGVSSDLSADPAWIQRVRTDGTAGGVFADYRYYGTGDIGGAPGETSVSMVE
                     KSAAGNGPLTVISGPADRMFDDITPHQAAGLPQYRGDLLLTNHSAGSISSEAYQKRWN
                     RKNELLADAAERASVAAQAIAGLSYPSARLANAWRLVLGGQFHDILAGTATPAAYEYS
                     WNDEVLAMNQFALVLSQATDALASSLDTQTRGTPVVVYNPLEIEREDVVEATIPFPEP
                     APRAVRVVGPDGSTVLAQVTSTDSAGTRVLFLARVPPLSYTVFDIQPADAAPESGLRV
                     SADRLENARYRIRIDANGDLSSVLDKLTGRELLAAPARLAFQTERPWDWPAWNMDWGD
                     QRRAPRGYVPGPARARIVENGPVRVAVQVDREAEGSRFQQTIRLSEGDAGNRIEIADS
                     IDWRSSAAALKATFPLTARNPLATYSWDVATVQRGNNDSRKFEVPSHEWFDLTDRGGQ
                     FGVTVLSDCKYGSDKPDDSTLRLTLLHTPGIGGGNGHEYADQTTQDWGHH"
BASE COUNT          548 a         1141 c         1131 g          561 t
ORIGIN      
        1 gcgcccgcgt ctgcctgtcc tgagtagacg gcggtggcaa ccccaggatg gctcccggcg
       61 tccagcacct tcatgtccac ttccggaatg agtccggcgg ccctgatcat gaggctgggg
      121 atgatccagc cgctggccga gaggggatcc acccgggcga agctgtggcc cttgaggtcc
      181 gccagcgtct tgatgccggt gtccggcctc gtgatgatct ctccctggta gaacgcggcc
      241 ccgttccgca cggagaccag ggcggcctgg gaaacgccgg agtccgccgc caggatatag
      301 gcggcggtgg ccagggacgt gatctgggcg cgcggcgggc tcgccttcag ggcctcgatg
      361 acgccgacgt agtccgttgc cacgaacgtc ttgaagaaga gtcccgtctt cgcgtgcagc
      421 aggtccgcga ccgactgtgc gccggcggac acctgcgcgg tgtcgcccga gggcacgtaa
      481 gcccagatga acgggttggt ctcgctgccg agcggatccg ccgcccaacc gcccatcgcc
      541 gtgaccgcga gcgcggccag acacaggaac gcgattctcc tggacacggg atacctcctt
      601 ggtgggatga cgtgcgctca gagtacggca cgccgcccca tccgtcaagc aaggtcggtc
      661 gacgacgcgg gaaaaggagc gtacactacc cttgagatgc ggaaagcggt ctgcctcctt
      721 ctcctggttg cctgtccctt tctggccgcg gcccaagacg agacgcgggc cggacccgcc
      781 tacaccgtgc aaagcccggg caagtacctg cacttcctgg ccggcatgtc cctggggatg
      841 cttggggcgg ggcttgcgga gtacgccctt cagccgacct acccgtccca gtatccgctc
      901 gtgctcccct ccgtcgccat ttccgtcgcg ttgcccgcgg gcatcgccaa ggagctcctg
      961 gacgccacgg gcttcggcga cgctcggctg acggacgtct tgatcaccac cgccgggggc
     1021 cttctggcct ccatgatggt cgggtatgcg gaagcgctgt accccgccaa cagccgaggg
     1081 aagagcaaca gcgtggccct gctcctctcg acgtcggccc tgctggccgt gccggttgcc
     1141 atcggcttcg ctcgggaggt ccggaacaac ctggagaagc gcagatgagg caagcatccc
     1201 gtcggtcgac gctcggcgcc ctgttcctgg cggtcgcggt ctcgacggcc gccgctcctc
     1261 gggatctctc gtcggccccg accctttacg tggtcgggta cgctcatctg gacaccgagt
     1321 ggcgatggga gtacccgcag gtgatccgtg agttcctgcc gaacaccctg agacggaact
     1381 tcgctctcct ggaccggtat ccgcactacg tcttcaactt cacgggctcc aaccggtatc
     1441 agatgatcca ggagtacttc cccgcggact tcgcgcggct gaagggctac gtgggcgccg
     1501 gacgctggtt ccccgccggc tcatcggtgg aggagagcga cgtcaacgtt ccctcggcgg
     1561 agtcgatcct tcgccaggtc ctctacggga acgagttctt ccgaagggag ctggggaccc
     1621 agagcgacga atacatgctc ccggactgct tcggctttcc cgccacgctc ccgagcctcc
     1681 tggcgcatgc gggcctccgg gggttttcca cccagaagct cgtctggggc tccgcggcgc
     1741 cggtcggtgg catcgggtcc ccggagcaga cgcctcgggg gataccgttc aacgtgggca
     1801 cgtggatcgg tcccgacggc aggggagtga tcgccgctct gaaccccggt gactacacgt
     1861 cgggagtcag ctcggatctg agcgcggatc ccgcctggat ccaacgggtc cggaccgacg
     1921 gaaccgccgg cggggtgttc gcagactacc gttactacgg caccggggac atcggtggcg
     1981 ctccgggtga gacatccgtg agcatggtgg agaaaagcgc ggcgggaaat ggaccgctca
     2041 cggtgatctc gggtccagcg gaccggatgt tcgatgacat cacgccgcac caggcggccg
     2101 ggctgccgca gtaccgggga gacctgctcc tgaccaacca ctccgccggc tccatcagct
     2161 ccgaggcgta ccagaagcgg tggaatcgaa agaatgagct tctcgcggac gccgcggaaa
     2221 gggcttccgt ggcggcccag gcgatcgcag gtctgagcta tccctcggcg cgccttgcca
     2281 acgcatggag gcttgtcctg ggcggtcagt tccacgacat cctggccggc acggctactc
     2341 cggccgccta cgagtattcc tggaacgacg aggttctggc catgaaccag ttcgcgctgg
     2401 ttctttcgca ggcaaccgac gcgttggcct cctccctgga cacccagacc cgagggacgc
     2461 cggtcgtcgt ctacaaccct ctggagatcg aacgggaaga tgtcgtcgaa gcgaccatcc
     2521 cctttcccga gccggcgccg cgggccgtcc gtgtggtcgg gccggatgga tctaccgtct
     2581 tggcccaggt cacgagcacc gacagcgcgg gcacccgcgt gcttttcctg gcaagagtgc
     2641 cgcccctcag ctacacggtt ttcgacatcc agccggcgga tgccgcgccg gagagcggcc
     2701 ttcgcgtcag cgccgaccgt ctggaaaatg cccgctaccg gattcgtatc gacgcgaatg
     2761 gagacctttc cagcgttctc gacaagctga cggggcgtga gctcctggcc gcgcccgcgc
     2821 ggctcgcctt ccagacggag cggccgtggg actggcccgc gtggaacatg gactggggcg
     2881 atcagcgccg ggcgccgcgg ggctacgtcc ccggcccggc gcgggcgcgc atcgtcgaga
     2941 acggtccggt gcgggtcgcg gtccaggttg accgcgaggc ggaaggctct cggttccagc
     3001 agaccatccg gctttccgag ggagatgcgg gcaaccgcat cgagatcgcc gattcgatcg
     3061 actggcggag cagtgccgcg gccctgaagg caacgttccc tctcacggcg cgcaatccgc
     3121 tggccaccta cagttgggat gtcgcgacgg tccagagagg caacaacgac tcccggaagt
     3181 tcgaagtgcc gtctcatgag tggttcgatc tcaccgaccg gggtgggcag ttcggggtca
     3241 cggtcctctc ggactgcaag tacggctccg acaagcccga cgactccacc cttcggctga
     3301 cgctcctgca cacgccaggg atcgggggag gaaacggcca cgagtacgcg gaccagacga
     3361 cccaggattg gggtcaccac g
//
LOCUS       DASRZS010000572         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5322795, whole genome shotgun sequence.
ACCESSION   DASRZS010000572 DASRZS010000000
VERSION     DASRZS010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5322795"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFH83_13955"
     CDS             <1..375
                     /locus_tag="VFH83_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487526.1"
                     /translation="LDALAEPWSGHDGAVFPVIDARKGRIYTARFEGGIRRGEYLDEP
                     PARVRSLIESARRPLLAGPDAERIRGLLDLAPDQVRTSELVDPMSLLRIGTKRYTTEG
                     ADPARLRPLYLRKSEAEITSGM"
     gene            378..1493
                     /locus_tag="VFH83_13960"
     CDS             378..1493
                     /locus_tag="VFH83_13960"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6487527.1"
                     /translation="MSDEPDRTDTEEVNEAEELDSQPSASSSSLLQSAEFSQWEATYL
                     QRALPCLFLDSSLRIVAANERFGRQFGSSAPVPGQYLTQFYAPSFNDALSAELFRSVR
                     SPKDSYSWHGKVEKIGSDQLLNIFKIWVLPLRSAGNGGLPPGRPSPDQDRLPAAYSAV
                     CMDMTLEYRQLISSTFSSLLEAARLKDNDTGNHIERVNLYAHVLAESLAAGRQYPEVD
                     PQFVNNIGLVSALHDVGKIGTPDDILNKAGRLEAWEWDVMKQHTTNGAFILSTYPVPM
                     ASEIALRHHERWDGTGYPHGLSEELIPLSARIVAVCDVYDAMRMRRAYKEPMSHEDTV
                     SHILKGKGSHFDPRLVDQFMPISDRFRDIFSELADPQ"
     gene            complement(1645..1827)
                     /gene="csrA"
                     /locus_tag="VFH83_13965"
                     /pseudo
     CDS             complement(1645..1827)
                     /gene="csrA"
                     /locus_tag="VFH83_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
     gene            complement(1821..2273)
                     /gene="fliW"
                     /locus_tag="VFH83_13970"
     CDS             complement(1821..2273)
                     /gene="fliW"
                     /locus_tag="VFH83_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HET6487528.1"
                     /translation="MIARTKPYGEIEIADRQCLHFPGGLFGFEDLTEFALLDAQQAPF
                     YWLQSLQRVEVAFVLLDPRFFRPDYTPDVDTQETAQIEIQSEDDLLVFAIVTIPDDAS
                     RMTANLQGPILVNRRLRVGRQSISLNPRWGVRHGVMEELARAQQGAAC"
     gene            complement(2277..>3174)
                     /gene="flgL"
                     /locus_tag="VFH83_13975"
     CDS             complement(2277..>3174)
                     /gene="flgL"
                     /locus_tag="VFH83_13975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HET6487529.1"
                     /translation="LGELLSIGNSKDQAGNYLFSGTQTKTEPFRITEGRIAGGSGDRV
                     VGVDYLGNVDSGVAEISEGATAQTHLIGTRVFWAEQQQIYSTVDAGQYRVQRDSAIRI
                     DGTQIQLTTGDTVSAVVAKINDANVAVRARIDPVANGLVLETTSAHQIAAEDLGGGTV
                     LQDLGILAPGGTHPPLNVSPTAHVFGGSVFDQIIALRDALYEGSSEKVGTAGLRGIEN
                     AVTAMAGSLGDLGARDARLDVVGKRVAYMTPDLQGIESQIHDLDMADAITRLKTLEST
                     NEAALSATARALNRPKLLDFLR"
BASE COUNT          518 a         1081 c         1039 g          536 t
ORIGIN      
        1 ctggacgccc ttgcagaacc gtggtccggc catgacgggg cggtgttccc ggtgatcgat
       61 gcccgcaagg gtcggatcta cacggcccgg ttcgagggag ggatccgccg tggggagtac
      121 ctggacgagc ctccggcccg ggtccgctct ctgatcgagt ccgcgcggcg accgctcctg
      181 gcggggccgg acgcggagcg gatccggggg ctcctggacc tcgctccgga ccaggtgagg
      241 acgagcgagc ttgtggaccc gatgtcgctc ttgcggatcg ggacgaaacg ctatacaacg
      301 gagggcgcgg acccggcgcg gcttcggccg ctgtacctgc gcaagagcga ggccgagatc
      361 accagcggaa tgtgacgatg agcgatgagc cggaccgaac cgataccgaa gaggtcaacg
      421 aggcggagga gctggattcc cagcccagtg cctcgtcgtc ttcgcttctc cagtccgccg
      481 agttctccca gtgggaagcg acctacctcc agcgcgccct accctgcctc tttctggaca
      541 gcagcctgag gatcgtcgcg gcgaacgagc gcttcggccg ccagttcggc tcctcggctc
      601 ccgtgccggg acagtatctc acccagtttt atgccccgtc cttcaacgat gcattgagcg
      661 ccgagctctt ccggtccgtc cggtccccca aagacagcta tagctggcat gggaaagtgg
      721 agaagatcgg aagcgaccag ctcctgaaca tcttcaagat ctgggtcctg ccgctgcgct
      781 cggcgggaaa cggcggtctc cccccgggaa gaccttcccc ggaccaggac cgcctgcccg
      841 cggcctacag cgcggtgtgc atggacatga ccctggaata ccgccagctc atctccagca
      901 ccttctccag cctcctggag gctgcccggc tcaaggacaa cgataccggc aaccacatcg
      961 agcgggtcaa cctctacgct cacgtgctgg cggagtccct ggccgccggg cggcagtatc
     1021 ccgaggtaga tccgcagttc gtgaacaaca tcggactggt ctccgcgctc cacgacgtgg
     1081 ggaagatcgg aacgccggac gatatcctca acaaggcggg caggttggag gcgtgggagt
     1141 gggacgtcat gaagcagcac accacgaacg gcgccttcat cctctccacc tatcccgtgc
     1201 ccatggccag tgagatcgcc cttcggcatc acgaacggtg ggacggcacc ggctaccccc
     1261 acggtttgag tgaagagctg attcccctct cggcgcggat cgtggcggtg tgtgacgtct
     1321 atgatgccat gcgtatgaga cgcgcgtaca aggagccgat gtcgcatgag gacacggtgt
     1381 cgcacatcct gaagggaaag ggctcccatt tcgaccccag gctcgtggac cagttcatgc
     1441 ctatctcgga ccgattccgg gacatcttca gcgagctcgc ggacccccag taggtccccg
     1501 gcctccgccg cccgccttgg gaccacgggg cggctggccc ggctgctccg gcccaccacc
     1561 cgacgaaggt ccgctccccg gcgccgtcgc gcccggggcc ttcccgccca gcagcccctc
     1621 caggccgtgc aaggcatccg gggacgccga ggccgcggcg cggttctccg cctggatggc
     1681 ggcatagacc tcccgccggt acaccttgac ttgcggcggc gccgtgatcc ccagcttgac
     1741 ctggtccccc ttgatgtcga tgaccgagat ctcgatctgg tccgcgacga tgatgctttc
     1801 cccgatgcgt cgggccagga tcagcatgcg gcgccctgct gcgcccgcgc cagctcctcc
     1861 atgacgccgt ggcggacgcc ccagcgcgga ttgaggctga tggactggcg gcccacgcgc
     1921 aggcggcggt tgaccaggat gggcccctgc aggttcgcgg tcatgcgcga ggcatcatcc
     1981 ggaatcgtca caatggcgaa caccaggagg tcatcctccg actggatctc gatctgggcc
     2041 gtctcctgcg tgtccacgtc cggcgtgtag tcggggcgga agaaccgcgg gtccagcagg
     2101 acgaaggcca cctccacgcg ctgcagggac tgcagccagt agaacggcgc ctgttgggcg
     2161 tccagcaggg cgaactcggt aaggtcctcg aacccgaaga ggcctccggg gaagtggagg
     2221 cattggcggt ccgcgatctc gatctctccg tacggtttgg ttcgcgcgat catgggctac
     2281 ctcagaaagt ccaggagttt cgggcggttc agggcccgcg cggtggctga cagcgcggcc
     2341 tcgttggtgg actccagcgt cttcagtcgg gtgatggcgt ccgccatgtc caggtcatgg
     2401 atctgggact cgatgccctg aaggtccgga gtcatatacg ccacccgctt gccgaccacg
     2461 tccaggcggg cgtctcgtgc gccgaggtct cccagggacc ccgccatggc cgtcaccgcg
     2521 ttctcgatgc cgcgcagccc cgccgtgcca accttctccg aggacccctc gtagagggca
     2581 tcccgcagag cgatgatctg gtcgaacacc gatccgccga acacgtgcgc ggtaggagag
     2641 acgttcaggg gcggatgagt cccgcccggc gccaggatgc cgaggtcctg gagcaccgtc
     2701 ccaccgccca ggtcctccgc cgcgatctgg tgcgcggagg tggtctccag caccagcccg
     2761 ttggccaccg gatcgatccg ggcgcggacg gccacgttgg cgtcgttgat cttggccacg
     2821 accgccgaca cggtgtctcc cgtggtgagc tggatctgcg tgccgtcgat ccgtatggcg
     2881 gagtcccgct gcacccggta ctgcccggca tccaccgtgg agtagatctg ctgctgctcg
     2941 gcccagaaga cccgggtccc gatgagatgg gtctgggccg tggctccctc ggatatctcc
     3001 gctacgcccg agtcgacatt cccaaggtag tcgacgccca ccacgcggtc tccgctcccg
     3061 ccggcgatcc gtccctccgt gatccgaaac ggctcggtct tggtctgcgt gccggagaag
     3121 agatagttgc cggcctggtc cttggagttg ccgatggaaa ggagctctcc cagg
//
LOCUS       DASRZS010000573         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1481660, whole genome shotgun sequence.
ACCESSION   DASRZS010000573 DASRZS010000000
VERSION     DASRZS010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481660"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VFH83_13980"
     CDS             complement(<1..586)
                     /locus_tag="VFH83_13980"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET6487530.1"
                     /translation="MLPLADCAVGGVGSIQVSSTPPGASIVLDETDSGLTTPATLTGI
                     VAGFHTVRLKLAMESDWGPKSIQVISGKTVQVGGTLRYPGGLVFLPQEQYDSMPPLRP
                     TGAPTELPARVDLRNLFPPPGDQGLTQPSCVGWAVGYGLKTYQERLAHGWSLSSDSHL
                     MSPSFIYNQIREPGGGALLYKALELVLTEGDASLA"
     gene            777..1751
                     /locus_tag="VFH83_13985"
     CDS             777..1751
                     /locus_tag="VFH83_13985"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6487531.1"
                     /translation="MKYRNLGSTGMRVSEIGFGAWAIGGGWGPQQDADSLKALHAAID
                     SGVTFIDTAAGYGNGRSERLIGQVLKERKEEVLVATKTPPTAGQWPPSPYCVADERYP
                     ESYLRQSVDERLKNLGVERLDVLQLHTWTRAWNRDATPFHVLARLKKEGKLRAVGVST
                     PEHDQNAVVDLMRQGLVDTVQVIYNIFEQEPAAELLPVAKEKGVGIIVRVAFDEGSLT
                     GRFTPQTRFDKDDFRTRYFAGDRLERAVARAAEVQTDIAGSGYTLPQAALKFVLAHPA
                     VSVVIPGMRNVAQAQANAAVSGLPDMTEALQKKLHRHNWLRGFWYSGK"
     gene            complement(1809..>2482)
                     /locus_tag="VFH83_13990"
     CDS             complement(1809..>2482)
                     /locus_tag="VFH83_13990"
                     /inference="COORDINATES: protein motif:HMM:NF023435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6487532.1"
                     /translation="AFEQGKSAVAFGNSIDTQGNIAKSIDSGFDVEIIPVLTSTGHYP
                     QDSFINNGVALAATTKNPERTLQVLDKIMEDPSYVYLVYYGIEGTNYIVKDGKIDLPE
                     GLTADKNTYPIDQAGFWWVDKNLFKPLASWPAAYITLESKIGAMLYATPYAAFQFVPD
                     NVKSELANVTQVWQQYEWPLQVGMVDDVNQAVATLRDKLAAADSQKVQDELQKQMDAF
                     GASLK"
BASE COUNT          442 a          776 c          836 g          428 t
ORIGIN      
        1 tggccaggga cgcgtcgcct tccgtaagaa ccagctcgag ggccttgtag aggagcgcac
       61 cgccgccggg ctcccggatc tggttgtaga tgaaggatgg gctcatgaga tgagagtcgg
      121 agctcaggga ccaaccgtgc gcgagccgtt cctggtaggt cttcaagccg tatcccaccg
      181 cccaacccac gcaggagggc tgggtcaatc cctggtcccc gggcggcgga aacaggttcc
      241 gcagatccac gcgggccggc agctccgtgg gagcgcccgt tgggcgcagc ggaggcatgg
      301 agtcgtactg ctcctgaggc aggaacacca gaccccctgg atagcgcaga gtgcccccaa
      361 cctggacggt tttgccgctg atgacctgga tcgatttcgg accccaatcc gactccatgg
      421 cgagcttcag tcgtacggta tggaagcccg ccacgatgcc ggtcagcgtt gccggagtcg
      481 tgagcccgga gtcggtctca tccaggacga tcgatgcgcc cggcggcgtc gagctgacct
      541 ggatgctccc cacgcctcca accgcgcagt ccgcgagcgg caacacgtaa aagaggattg
      601 cgcaacggat ccaccccggc cgagacactt tctgactccg gacaatcggt ctttcctcta
      661 ttggatactc gcggtccgga ttccgcaagt accggttgac ggtgatggag aacggctccg
      721 aatcccgaga aagccgcatt acgctaaaat cgctatcttc cgttgggagg tgaagcgtga
      781 agtatcgaaa ccttgggagc acgggaatga gggtgtccga gatcgggttt ggggcctggg
      841 ccatcggagg gggctgggga ccccagcagg atgccgattc cctcaaggcc ctgcatgccg
      901 ccatcgatag cggggtcacg ttcatcgaca cggccgccgg ctacggcaac ggacgcagcg
      961 agcgtctcat cggccaggtc ctgaaggagc gcaaggagga ggtactcgtc gccaccaaga
     1021 ccccgcccac cgcggggcag tggccgcctt ccccctactg cgtcgcggac gagcgctacc
     1081 ccgaatccta tctgaggcaa agcgtggacg agcggctcaa gaaccttggc gtagagcggc
     1141 tggacgtgct tcagctccat acctggaccc gggcctggaa ccgcgatgcg actccattcc
     1201 acgtcctggc gcgcctcaag aaggagggga agctgcgagc cgtcggggtg tccacgccgg
     1261 agcacgacca gaacgcggtg gtggatctga tgcgccaggg gctcgtggat accgtccagg
     1321 tgatctacaa catcttcgag caggagcccg ccgcggagct tctgcccgtc gccaaggaga
     1381 agggcgtggg gatcatcgtg cgcgtggcgt tcgacgaggg gtccctgacc ggtcggttca
     1441 ccccgcagac ccggttcgac aaggacgatt tccgaacgcg ctacttcgcc ggcgaccgac
     1501 tggagcgcgc cgtggcgcgg gcggccgagg tccagaccga catcgcggga agcggctaca
     1561 ccctgcccca ggcggcgctc aagttcgtcc tggcgcaccc cgccgtgagc gtggtcatcc
     1621 ccggcatgcg gaacgtggcg caggcccagg ccaacgccgc ggtcagcggg ctcccggaca
     1681 tgacggaagc gctccagaag aagctgcacc ggcacaactg gctgcggggg ttctggtact
     1741 cggggaagta ggcgggagtc cttggagggg gctcggtccc gccggtgcgg ggccgagccc
     1801 tgggaccgtt acttcagcga cgctccgaag gcgtccatct gcttctgaag ctcatcctgc
     1861 actttctggg aatccgccgc ggccagcttg tctctcagcg tggcgacggc ctggttcacg
     1921 tcatccacca tgccgacctg aagcggccac tcgtactgct gccagacctg cgtgacgttg
     1981 gccagctcgg acttcacgtt gtccggcacg aactggaagg ccgcgtacgg cgtggcgtac
     2041 agcatggcgc cgatcttcga ctccagcgtg atataggccg cgggccagga ggccaggggc
     2101 ttgaacaggt tcttgtccac ccaccagaac ccggcctggt cgatggggta ggtgttcttg
     2161 tccgcggtca gtccctccgg caggtcgatc ttgccgtcct tcacgatgta gttcgtgccc
     2221 tcgatgccgt agtacacgag gtacacgtag gacgggtctt ccatgatctt gtcgaggacc
     2281 tggagggtgc gctcggggtt cttggtggtg gctgccagcg ccacgccgtt gttgataaag
     2341 gagtcctggg gatagtgccc ggtggaagtc aggacgggga tgatctcgac gtcgaaaccc
     2401 gagtcgatgg acttggcgat gttgccctgg gtatcgatgg agttgccgaa ggccaccgcg
     2461 gacttgccct gctcgaacgc ct
//
LOCUS       DASRZS010000574         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_317287, whole genome shotgun sequence.
ACCESSION   DASRZS010000574 DASRZS010000000
VERSION     DASRZS010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317287"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VFH83_13995"
     CDS             <1..464
                     /locus_tag="VFH83_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487533.1"
                     /translation="ELKFWEDLRILPRDLTVQAIVRISETTSGSGGTSWALSRGPDSR
                     GIPRWSFPGSQDAVQASQADAVAGALLAVEGEDFLTDDRAAEAGPVATVTVSVNDGRD
                     FRLSFGPKTADSRYPCRLQDGTVVYLVPQWRYQQIVVDRSTLRAQTRAGAP"
     gene            complement(433..1659)
                     /locus_tag="VFH83_14000"
     CDS             complement(433..1659)
                     /locus_tag="VFH83_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487534.1"
                     /translation="MNKRVSWGFLAVSGAFFLFIVGLTGYRIEDVRRLDTAMVRGRFP
                     DLANRVTRAHQGDGAWDSPQLKSDLRAIFDGEPRLLLLAIHSPTDGILYLVSRNRWYL
                     RDPAEITPEWRGTPSYQTSRGYEDLLSQELPGEAGSPTLDAIYVIMGREDLYPIVRDD
                     LYLFLAFLLVCGVFALIVTSSQQAEYEQAGPRARPTHSPLDPDPRNTPPRNQNPGNLH
                     SQGPNPASAAGRAPASGAATETPPVHPAGASGPGHAPDSMMTRLQAELQRTEAADEDL
                     SLARVRVDPKVGDQEAAYRELGIALREQFMLVDLLFEDSDRSYSIILPDVDLDDALRT
                     LDDLRKKIGAQTGGLPRSISAGASARSGRLIDAKELREEADISLAKAIQEGGDQVIGF
                     RADAARFRGLLRESAL"
     gene            1750..>2549
                     /gene="amrB"
                     /locus_tag="VFH83_14005"
     CDS             1750..>2549
                     /gene="amrB"
                     /locus_tag="VFH83_14005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HET6487535.1"
                     /translation="MRVRRRYLPPGWYPGNAEVARRKIEEMEEGIRPPSAAGIAGVVP
                     HAGWEFSGRLALEVLKRLSPSMDTMVIIGGHLGPADGLLCAFEDSYDTPLGPVAADLE
                     LMEAIGSSIPLREDRYADNTVEVQLPFVRYLFPEVRVLGMRASPSRDAARLGVVVSDA
                     AKRLGRRVAVIGSTDLTHYGAGYGFQPAGIGEHALKWVREVNDRRLIDSLLAMDLDAS
                     LSRALGERSACSAGGALAALSFAKTNGIQSGTLVCYATSQDVHPSESFV"
BASE COUNT          413 a          827 c          874 g          435 t
ORIGIN      
        1 cggagctgaa gttctgggag gacttgcgga ttctgcccag ggacctgacc gtccaggcga
       61 tcgtgcgcat ctccgagaca acttccggct caggaggcac ttcctgggct ctgagtcgtg
      121 gaccggacag ccgggggata ccgcgctggt cctttccagg atcccaggac gcggtccagg
      181 catcccaggc ggatgccgta gcgggtgcgc tccttgccgt ggaaggggag gatttcctga
      241 cggatgaccg agcggcggag gcgggaccgg tcgccaccgt gaccgtgtcg gtgaacgacg
      301 gccgcgactt ccggttgagc ttcggtccca agacggctga ctcccgctat ccctgtcgct
      361 tgcaggacgg gacggtggtt tacctggtgc cgcagtggcg ctaccagcag atcgtggtgg
      421 accggtcgac cctcagagcg cagactcgcg caggagcccc ctgaaccggg ccgcgtccgc
      481 gcggaacccg atgacctggt cgcccccctc ctggatggcc ttggccaggg agatgtcggc
      541 ctcctcgcga agctccttgg catcgatcag tcttccgctt cgagcgctgg cgccggcgga
      601 gatggaccga ggcaggccgc ccgtctgggc gccgatcttc ttccgcagat catcgagcgt
      661 cctcagggcg tcgtccaggt cgacatccgg caggatgatg ctgtacgagc gatcggaatc
      721 ttcgaacagg aggtccacca gcatgaactg ctcccgcagg gcgatgccca gctctcggta
      781 cgctgcctcc tggtccccca ccttcgggtc cacccggacc cgggcaaggc tcaggtcctc
      841 atccgcggct tccgtccgct ggagctccgc ctggagccgg gtcatcatcg aatcgggcgc
      901 atggccgggc ccggaggcac ccgcgggatg caccggtgga gtctcggtcg ccgctcccga
      961 ggcaggagcg cggcccgcgg cggaggccgg gttcggaccc tggctgtgca gattgccggg
     1021 gttctgattc cttggaggcg tgttccgggg gtctggatcg agcgggctat gcgtgggacg
     1081 agcacgcggg ccggcctgct cgtactccgc ctgctgggag ctggttacga tcagggcgaa
     1141 aacgccgcag accaggagga acgccaggaa caggtacaga tcgtcccgaa cgatcggata
     1201 gagatcctct cgacccatga tgacgtagat ggcatccagg gtaggggagc ccgcctcccc
     1261 cgggagctcc tggctcagca gatcctcgta tccgcggctg gtctggtagc tgggcgttcc
     1321 ccgccactcc ggcgtgatct cggccgggtc ccgcagatac cagcggttgc ggctcaccag
     1381 gtagaggatg ccatccgttg gagagtggat ggccagcagg agcagccgcg gctccccgtc
     1441 gaagatggcc cgaaggtcgg acttgagctg gggcgaatcc cacgccccat ctccctggtg
     1501 agcccgggtc acccggttcg ccaggtctgg aaaccgtccg cgcaccatgg cggtatccag
     1561 gcggcgcacg tcctcgatgc ggtatccggt cagccccaca atgaaaagga aaaaagcgcc
     1621 gctgaccgca aggaatcccc acgaaacacg cttattcatg ctcctctccc gatccaaagt
     1681 ggagtatact gtcgccggac gattgggcac aggtcaggac ttggcatcac cgcttctgcg
     1741 gagggtccta tgcgagtgcg aagaaggtac ctgccaccgg gttggtatcc gggaaacgcc
     1801 gaggttgccc ggcggaagat cgaggagatg gaggaaggca tacggccccc gagcgccgcg
     1861 ggaatcgccg gcgtcgtccc ccacgcgggg tgggagttct cgggccgcct cgccctcgag
     1921 gtcttgaagc gtctttcgcc cagcatggat accatggtaa ttatcggcgg ccacctgggc
     1981 ccggctgacg gattattgtg cgccttcgag gactcgtacg acacgcccct gggaccggtg
     2041 gcggcggacc tcgagctcat ggaagccatc ggctcatcga tcccccttcg ggaggaccgc
     2101 tacgccgaca atacggtgga ggtgcagctt ccatttgtcc gctacctctt tccggaggtc
     2161 cgggtgctcg ggatgcgcgc atccccgtcg cgggacgcgg cacggctggg cgtggtggtc
     2221 tccgacgcgg cgaaaaggct cggtcggcgc gtcgcggtga tcggctcgac ggacctgacc
     2281 cactacgggg ccgggtacgg gttccagccc gcgggcatcg gcgagcacgc gctcaaatgg
     2341 gttcgcgagg tcaacgaccg gcggctgatc gacagcctcc tggccatgga cctggatgcg
     2401 tccctgagca gggcgctggg agagcgatcc gcttgctccg ccggcggcgc ccttgcggcc
     2461 ctcagcttcg cgaagacgaa cggcattcag tcggggaccc tggtgtgcta tgccaccagt
     2521 caggacgttc acccttcgga atccttcgt
//
LOCUS       DASRZS010000575         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_697989, whole genome shotgun sequence.
ACCESSION   DASRZS010000575 DASRZS010000000
VERSION     DASRZS010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697989"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VFH83_14010"
     CDS             <1..1116
                     /locus_tag="VFH83_14010"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6487536.1"
                     /translation="RSGSISVAVVRNGAIVYRFAAGASGQGTSATPATAYHWGSMTKL
                     VTASAVMRLVETNKIALDAPVERYLPEFPKGLGITVRNLLDHSSGLPDPEAAQLVSFG
                     GSTLPPLQEVLASYLADFHGPSFPPGSDAAYSNWNYLVLGVIIERVTGIPYATYVTET
                     ILRPLGMTHTAFRAIDLPEGARLATPVMATASEAQLIDLLNTMRPRKDAETMVLGRSG
                     GSTYLGTFDILAPWGGLAGTAEDVARFLGMSLGSPPPGSEQVLSKATAASMRKVQRSS
                     TGRALPWALGWMLRPEKGEEVVEQGGGGPGIDDLMRVYPRRHLGVVVMGNANNYGASR
                     ILSAAAEIFSAVDDAEGIPRTLESRISGSLPRASVHP"
     gene            complement(1140..2420)
                     /locus_tag="VFH83_14015"
     CDS             complement(1140..2420)
                     /locus_tag="VFH83_14015"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6487537.1"
                     /translation="MSLAEPLRASVRRMGFERPTPIQSAAIPPALAGRDVLASAETGS
                     GKTAAFLLPIMNRLLALQRGATRALVLAPTRELAAQIAEHFKQLAVGTRLTCATVHGG
                     VGMGNQERAFRKGVDVIIACPGRLLDHMKRPHVDFSSLEHLVLDEADRMLDMGFLPDI
                     KRVLAAIPKDRQTLFFSATMPPAIIDLSRDMLQDPERINMDRKPAPAVGIHHAAYPVA
                     SDRKASLLLELLGGDISGSVLVFTRTKHRANRLADFLESHGVPCARIHGNRSQSQRTT
                     ALDGFKKGSYRVLVATDIVARGIDVEALGSVVNFDVPHQPDDYIHRVGRTARAELTGD
                     AYTLFSPEEEKEFTAIERALGKLLPRRKLQGFDYTVPSAPADPQRRASPAGARGRSPA
                     SVRSSAPGRASRGPASKQVRNGHARQSAWRHVSN"
     gene            2691..>2920
                     /locus_tag="VFH83_14020"
     CDS             2691..>2920
                     /locus_tag="VFH83_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487538.1"
                     /translation="MQKIGYGEGSGHTDAQTAGRSKVVRPKAARLGWLLVGWLILAAS
                     PLSAQERITYRDRTGNQEITIAIQEEHQSQGFV"
BASE COUNT          506 a          923 c          993 g          498 t
ORIGIN      
        1 cggtccggaa gcatctccgt ggcggtggtc cgtaacggcg caatcgtcta ccgtttcgcg
       61 gccggcgcga gcggtcaggg cacgtctgct acgcccgcaa ccgcgtacca ctggggatcg
      121 atgaccaagc tggtcaccgc ctcggcggtg atgcggctcg tggaaaccaa caagatcgcc
      181 cttgacgcgc cggtggagcg ctatcttccc gagttcccga aagggctcgg gatcacggtg
      241 cgcaaccttc tcgatcatag ctcgggactg cccgaccccg aagcggccca gttggtctcc
      301 ttcgggggaa gcaccctccc tccgctgcag gaagtccttg cgtcgtacct cgcggacttc
      361 cacggaccga gcttcccgcc aggctccgac gcggcgtaca gcaactggaa ctacctggtc
      421 ctcggcgtga tcatcgagcg cgtaaccggg atcccatacg caacgtacgt gaccgagacg
      481 atcctgaggc ctctgggcat gacgcacacg gcgttccgcg cgatcgatct accggaaggg
      541 gcacgccttg ccacaccggt catggccacg gcctctgagg cgcagctcat cgatctcctc
      601 aacaccatgc gccccaggaa ggacgcggag accatggtcc tgggccggtc gggaggaagc
      661 acgtacctgg ggaccttcga catcctggcg ccttggggtg gcctggcggg taccgcggaa
      721 gacgtcgccc ggttccttgg aatgagcctg ggttcccctc ctcccgggtc ggagcaggtg
      781 ctctcgaagg ccacggcggc ctccatgcgg aaggtccagc gatcgagcac gggccgggcg
      841 ctgccctggg ctctggggtg gatgcttcgc ccggagaagg gagaggaagt ggtggagcag
      901 ggaggcggcg ggcccggcat cgacgacctg atgcgcgtct atccaagacg acacctcggt
      961 gtcgtggtca tgggcaacgc aaacaactac ggcgcctcgc gcattctctc agccgccgcg
     1021 gagatcttct ccgctgtcga tgacgccgag ggtatcccgc ggacgctgga gtcccggatc
     1081 tccggttcgt tgccacgcgc atcggtgcat ccctaaggac gccggtcccg tggaccgcgc
     1141 tagttcgaga cgtgcctcca ggcggattga cgggcgtgac cgttacgaac ctgcttggat
     1201 gccgggccgc ggctggcacg ccctggagcc gaggatcgga ccgacgcggg cgatcggccg
     1261 cgcgctcccg ctggggatgc tctcctctgg gggtcggctg gcgcggacgg gacggtatag
     1321 tcgaagccct gcagcttccg acggggcagg agcttgccca gcgcgcgctc gatcgccgtg
     1381 aactccttct cttcctcggg agagaacaga gtataggcgt caccggtcaa ttcggcccgc
     1441 gccgtgcgtc ctactcggtg gatgtaatca tcgggctgat gaggtacgtc gaagttgacc
     1501 acggagccca gtgcctccac gtcaatgcca cgggccacga tatccgttgc caccagcacg
     1561 cgatagctgc ccttcttgaa accgtccagc gccgtcgtgc gctgggattg actgcggttg
     1621 ccatggatcc gggcgcatgg caccccgtgg ctctccagaa agtccgcgag gcgattggct
     1681 cgatgcttgg tccgcgtgaa caccagcacg cttccggaga tgtcccctcc cagaagctcc
     1741 aggaggaggc tggcctttcg atccgatgcc acggggtatg cggcatggtg gatgccgacc
     1801 gccggagcgg gtttccgatc catattgatg cgctccgggt cctgaagcat gtcccgagac
     1861 agatcgatga tggcaggagg catcgtggcc gagaagaaca gggtctggcg gtctttggga
     1921 atggcggcca ggacccgctt gatgtccggc aggaacccca tgtccagcat gcggtccgct
     1981 tcgtcgagca ccaggtgctc caggctggag aagtccacat ggggccgctt catgtgatcc
     2041 agaagccggc cggggcaggc aatgatgaca tccacgccct ttcggaacgc ccgctcctgg
     2101 ttgcccatgc cgacgcctcc gtgcaccgtg gcgcaggtca ggcgtgtgcc tacggcaagc
     2161 tgcttgaagt gctccgcgat ctgcgccgcc agctcgcggg taggcgcgag caccagcgcc
     2221 cgagtagccc cgcgctgcag cgcaaggagt cggttcatga tggggaggag aaacgcggcc
     2281 gtttttccgc tgccggtttc tgcgcttgcc aggacgtccc gtccggccag ggcgggcggg
     2341 attgcggccg actgaatggg ggttggccgt tcgaagccca tgcgccgcac cgaggcgcgc
     2401 agcggctccg caagagacat ttgggaaaag ctcatcgtga tcctttacag gtccggcagg
     2461 taagagtgat tgatcggtgg aatcgccggt gaggccgccg cgaggacttt caggccctgc
     2521 acactcgttc gatgatactc aggctccgct acgcggcgcg ctgatcatat gaaagaatac
     2581 cgacgacccg catggatgtc aaccggtcgg cggagcggag ccgtgggttg gatgccacgt
     2641 gcgctagcgc acagacacgc cgccgctcgt gatcgagagt gcagagagac gtgcagaaga
     2701 tcgggtacgg tgagggaagc ggccatacgg atgcgcagac ggcgggccgg tcgaaggtcg
     2761 tacgacccaa ggctgcccgg cttggctggc ttctggttgg ctggctgatc ctggcggcga
     2821 gcccgctgtc agcgcaggag aggatcacct accgagaccg gacggggaac caggagatca
     2881 ccatagccat ccaggaggag caccagtctc aagggttcgt
//
LOCUS       DASRZS010000576         7558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_319846, whole genome shotgun sequence.
ACCESSION   DASRZS010000576 DASRZS010000000
VERSION     DASRZS010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7558
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319846"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VFH83_14025"
     CDS             complement(<1..240)
                     /locus_tag="VFH83_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487539.1"
                     /translation="MSKGVTAAALAAFMMISVVRGDALHISLRRIVHAVDKTVDTIAQ
                     AAFSPYRAAMAPVQRGLTTLYERTMKSAVDRAVQQV"
     gene            381..1109
                     /locus_tag="VFH83_14030"
     CDS             381..1109
                     /locus_tag="VFH83_14030"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6487540.1"
                     /translation="MAKIVTYTSGFDPAPLVASLPGAERISVGTRHDLAARIVEERMV
                     CAVIVQVQRLDDETRRFLAALKTHFPLLETVAVASEPLAGLPPGILRVDPNDSAGAGA
                     LFRRRCEATEKERREKLRFDWPLQGELSVDGVEWTRLGVRSLSASGAFLHYEGSPERF
                     GSVADLRIEFEDTTLRTRCQILGSRLSSSNLPTGFSVRFTDLSESAARAIDSIVKNAL
                     MEALLHPEAEPRFPSLDEEDSFTL"
     gene            1347..2333
                     /locus_tag="VFH83_14035"
     CDS             1347..2333
                     /locus_tag="VFH83_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487541.1"
                     /translation="MPRYILRRAVFMVITLVSISIVTFIVIKLPPGDFLTRRIAQLES
                     TGQRVDASQVAALKKEYGLDVPVVAQYLLWDWKMLHGNFGMSFGRLQPVNKLIAERLT
                     LNLAISLFTLLFTYLLSIPIGIFSATHQYSVWDYLFTVLGFVGVSIPGFLLGLILMLF
                     LNRTLGISVGGLFSSEYITAPWSVGKFVDMMKHLPLPIVIIGASGTAWFIRVMRSCLL
                     DELPKQYVITARAKGLSETRLVWKYPVKVAVNPIISTISWQLPNIFSGETIIAIVLNL
                     PTMGPLLFEALLNQDMYLAGSIVMILGFLTVVGTFLSDMLLIALDPRIRLGN"
     gene            2343..3446
                     /locus_tag="VFH83_14040"
     CDS             2343..3446
                     /locus_tag="VFH83_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487542.1"
                     /translation="MLVARQDEEILAASHGKLMWRKFRKHKVAMVGIVVLFVLYGLAI
                     FCEFFSPYDIYERHMDFTYMAPQRIHFVDPSGRFHLRPFVYGMKMTLNTDTFERSYAE
                     DHGQRYEVRFFTGTYGYRFWGLIRSRVHLFGVRQPGTIFLLGTDELGRDLFSRTLYAA
                     RISLSVGLVGVFFSFILGSILGGISGFYGKSVDLFIQRLIEVLISIPTIPFWMALSAA
                     LPPHWPPVKVYFGVTIVLSVLGWCGLARIIRGKILELKEEDFVLAARVAGAGGGTIIA
                     RHLLPSFFSFLIVHLTLAVPSTILGETALSFLGLGIRPPAVSWGALLSSAQNVRSILL
                     YPWLLIPGLFVIATVLAFNAVGDGLRDAADPYR"
     gene            complement(3476..5782)
                     /locus_tag="VFH83_14045"
     CDS             complement(3476..5782)
                     /locus_tag="VFH83_14045"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET6487543.1"
                     /translation="MLLSGFLELIRRERLLVKLISSFLAASVVSVVLVSGILFAAYDR
                     RSMRDLAAVTQLALAETANRLDRTFTQAADIAFLVFKDPDVVSLMYTDGKDPLNLVRI
                     TAFVQNLMASDPYIDSVALCGRDTVLLSTSQSGETGEPPQTILSLARMPKVLVPKPRL
                     VQRANGGLVSLLTLAINESNSPNYVDSAVVVNLDADAIRKDLGADAAEAPQTIVVVDD
                     SATVLLGSNAAGFGTSIADEPYMRRIAADPRSSGFFLTEAKGRRYAVSFARAANDHYT
                     AISLTDYARYTAGISGLRGILLAASGGILIAVLGFGLVLSLRLYVPLNALFDGIRSRV
                     SASPEPRRNELEYVSGILARAMDAFSAAEHAADNDRMVVSALLRDLLTGPSALDPGDL
                     EEELRRRNVIPDQPGRFVVAVLRIDGVALLRRKLTAEAIRFQMASTGGIAAETLKSRV
                     SCVPTQAAEDLVALLLFVPDRAARWWQSHAAESLLRVQEAVRRMLGIELTLAMGDPVA
                     TLREARSSYRRAEALTLYRLVLPKNELITAESVQGFRDEPMDERGIEDTFRGLMRSTD
                     RPAFASAVDRLVGACRGLRIERQFEAFQAAARAAMTAGVGVMPPEGDSRSAAHMDAAI
                     RSAESYDELKEWLLSLYDANQKKLRELGSSRSADFLQKALDFIDKNYADSQLSANLLA
                     DKLAITPQYFSRLFNESVGSSFPEYLMGLRLEKARALLLSVPRLTIDEICRRVGLGNR
                     TYFATAFKRRFGVSPATFRQAGAASSLS"
     gene            6012..7325
                     /locus_tag="VFH83_14050"
     CDS             6012..7325
                     /locus_tag="VFH83_14050"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET6487544.1"
                     /translation="MKKGLAVALGGLMLLGATLAFGAPVTLRYALWDGVQRPAMEAAI
                     QQFQKDNPDIVVTTELTEWGDYWTKIQTGAAAGTVPDVFWGHVAYFASLADKGGLLDL
                     APYIARDKVDLSIYYPGLVDNWKYGGKQYGLAKDWDTICYAYNKEMTAKAGVDPTRMT
                     WNPSDGGTFLLALQKLTIDKNGKNAAQAGFDPNNVVQYGIAGINAGEIQTGWLPFAWG
                     NGAPGSVVLDRPFGTRFVMDNPATVAALQFYADLVNRYHVAPSASGNQSFVGGAQDLF
                     LAKKVAMYPAGPWTLSGTRAGATFAWDVAPLPAGPAGRVTPFNGLAHVVSSTTKSRDA
                     AWKLASFMDGPKGENIIASFAVVFPSVKSALSTYQKAFEGKAPASIHFYTDEAAGKTG
                     PYPQHLKWGQIWDVVQRQFDSSFSGGMSVQQAVAAAKADVDPLLQ"
BASE COUNT         1277 a         2431 c         2460 g         1390 t
ORIGIN      
        1 gacctgctgg acggcgcggt ccacggcgct cttcatggtc cgctcgtaga gcgtcgtgag
       61 tcccctctgc accggagcca tcgcggcgcg gtaggggctg aacgccgcct gcgcgatcgt
      121 gtcaaccgtc ttgtccaccg cgtggacgat ccttcggaga ctgatatgga gagcgtcgcc
      181 ccgcacgacg gatatcatca tgaacgccgc cagcgcggct gctgtcaccc ctttggacat
      241 tccgacctcc tgccaatttg gaccaggcca aggtactgct tcccgcggcc gtagggaaat
      301 ccgtcgtggt ctctggaggg ctcatcggaa agacggtgtt gccgtgccgc tggccgcacg
      361 atacgtttca tcattcggca atggcaaaga tagtcaccta cacctccggt ttcgatcctg
      421 cacctctcgt cgcctcactc cccggagccg agcggatctc cgtcgggacc cgacacgatc
      481 tggcggcacg gatcgtcgag gagcggatgg tctgcgccgt catcgtgcag gtgcagcgcc
      541 tggacgacga gacgcggcgc tttctcgcgg ctctcaagac gcacttcccc cttctggaga
      601 ccgtggcggt cgcttcggag ccgctggcgg gtctgccacc ggggatattg cgggtggacc
      661 cgaatgacag cgccggcgcg ggcgcgctct tccgtcgtcg ctgtgaggcg accgagaagg
      721 aacgtcggga gaagctgagg ttcgactggc cgctccaggg tgagctcagc gtggatggag
      781 tggaatggac gcgtctgggc gtgcggtccc tcagcgcgag cggcgcgttc ctgcactacg
      841 aaggctcccc cgagcggttc ggcagcgtcg ctgatctcag gatcgagttc gaggacacga
      901 cgttgcgaac gcgctgccag atcctgggct cgcggctctc atccagcaac ctcccgacgg
      961 ggttcagcgt ccgtttcacc gacctgtcgg aatccgccgc ccgcgcaatc gactctatcg
     1021 tgaagaacgc cctcatggag gcgcttctgc acccggaagc cgagccgcgc tttccctcgt
     1081 tggacgagga ggactccttc acgctctgaa ggctcgcgtt ggtcggcgag tgaacgtggc
     1141 gggccgcgtc tgagcgcgac gggtggcggg cggagtctgc cggccggcga agcggcgtcc
     1201 ccgcatcgcg cgcatcgccc gcatcggccg tgccttgacg ggcggggacg cgggcgggca
     1261 tcatcgggac tgcggccttt gtggcggacc ctccgaacgg agccgcgcca ggggaaccgg
     1321 caagaacgct agccagggag actcggatgc ccagatacat cctcaggcgc gccgtcttca
     1381 tggtcatcac gctcgtctcg atctccatcg tgaccttcat tgtgatcaag ctgccccccg
     1441 gggactttct cactcggagg atcgcccagc tcgagtccac cggccagcgc gtggacgcgt
     1501 cccaggtggc ggccttgaag aaggaatacg gactggacgt gcccgtggtg gcccagtacc
     1561 tgctctggga ctggaagatg ctccatggga acttcgggat gtccttcggc aggctccagc
     1621 cggtgaacaa gctcatcgcc gagaggctta ctctgaacct ggccatctcc ctgttcacgc
     1681 ttctcttcac gtacctgctc tccatcccga tcgggatctt ctcggcgacc caccagtatt
     1741 ccgtgtggga ctacctcttc acggtcctgg gattcgtggg ggtttccatc cccgggttcc
     1801 tcctgggcct catcctgatg ctcttcctca accgaaccct gggaatcagc gtcggcgggc
     1861 ttttctcctc ggagtacatc acggcgccgt ggagcgtcgg aaagttcgtg gacatgatga
     1921 agcatcttcc cctcccgatc gtgatcatcg gggcttccgg gaccgcgtgg ttcatccgcg
     1981 tgatgcgaag ctgcctcctg gacgagctgc cgaagcagta cgtgatcacc gcccgggcga
     2041 aggggctctc ggagacgagg ctggtctgga agtatccggt aaaggtggcg gtgaacccga
     2101 tcatcagcac catcagctgg cagcttccca atatcttctc cggtgagacg atcatcgcca
     2161 tcgtcctgaa cctccccacc atgggaccgc tgctcttcga ggcgctgctg aaccaggaca
     2221 tgtatctggc ggggagcatc gtgatgatcc tggggttcct cacggtcgtc gggacattcc
     2281 tgtccgacat gctgctgatt gcgctggatc cccgcatccg gctcggaaac taggagagcc
     2341 ccatgctggt agcccgccag gacgaggaga tccttgccgc ttcccacggg aagctgatgt
     2401 ggcgcaagtt ccgcaagcac aaagtggcga tggtcgggat tgtcgtgctg ttcgtgctct
     2461 atgggctggc gatcttctgc gagttcttct ccccgtacga catctacgaa cggcacatgg
     2521 acttcaccta catggcgccg cagcggatcc atttcgtcga tccgtcggga aggttccacc
     2581 tgcggccgtt cgtctacggg atgaagatga ccctgaacac ggacaccttc gagcggtctt
     2641 atgccgaaga ccatggccag cgctacgagg tgcgcttttt cacagggacc tacggctacc
     2701 ggttctgggg ccttatccgg tcccgggtcc atctcttcgg cgtgaggcag ccggggacga
     2761 tcttcctcct ggggaccgac gagctggggc gggacctgtt ttcccgcacc ctgtatgccg
     2821 cgcggatctc cctctcggtc ggtctggtgg gcgtgttctt cagcttcatc ctcgggtcga
     2881 tcctgggtgg aatctcggga ttctacggca agtccgtgga tctcttcatt caacggctta
     2941 tcgaggttct gatcagcatc ccgaccattc cgttctggat ggcgttgagc gccgcactgc
     3001 ctccccactg gccgccggtg aaggtctact tcggcgtgac catcgttctc tcggtcctgg
     3061 ggtggtgcgg tctggcgcgg atcatccgcg ggaagatcct cgagctgaaa gaggaggact
     3121 tcgttcttgc ggcccgggtg gcgggcgcgg gcggcgggac gatcattgcc cgccacctgc
     3181 tgccatcgtt cttcagcttc ctgatcgtcc acctgacttt agcggttccc agcaccatcc
     3241 tgggggaaac cgccctcagc ttcctgggac ttgggatccg cccccccgcg gtgagctggg
     3301 gcgcgctgct gagcagcgct cagaacgttc gatcgatcct gctgtacccg tggctgctca
     3361 tccctggcct gttcgtgatc gctaccgtcc tggcattcaa cgcggtgggc gacgggctgc
     3421 gggacgcggc ggacccgtac cggtagtgga gcaggcgtgg cggaggcgcg tctcgctagg
     3481 acagcgagct ggccgcgccc gcctgacgga acgtcgcggg cgagacgccg aagcgcctct
     3541 tgaacgcggt ggcgaagtag gtccgattgc cgaggccgac ccgccgacag atctcgtcga
     3601 tcgtcagtcg gggaacggac aggagcaggg cgcgcgcttt ctcgagccgc agccccataa
     3661 ggtactccgg aaagctcgat cccacggact cgttgaacag tcggctgaaa tactggggcg
     3721 tgatggccag cttgtccgca agcaggttcg cggaaagctg ggagtccgcg tagttcttgt
     3781 cgatgaagtc gagggccttc tggagaaaat ccgccgaccg ggatgagccg agctcgcgaa
     3841 gcttcttctg gtttgcgtcg tacagcgaga gaagccattc cttcagctcg tcatagcttt
     3901 ccgcgctgcg gattgcggcg tccatgtgcg ccgcgctgcg ggagtcgccc tccggcggca
     3961 tcactccgac cccggcggtc atcgcggcgc gtgcggcggc ctgaaaggct tcgaactgcc
     4021 tctcgattcg caggccgcgg caggcgccta ccaggcggtc cacggcggag gcgaacgccg
     4081 gccgatccgt cgaccgcatg aggccgcgga aggtgtcctc tattcctcgc tcgtccatgg
     4141 gctcatcccg gaacccctgg acgctctccg cggtgatgag ctcgttcttc ggcagcacca
     4201 gacgatagag cgtcagcgct tcggcccgac ggtagctgga acgagcctcg cggagggtgg
     4261 caaccggatc tcccatggcc agggtaagct caatccccag catccttcgc accgcttcct
     4321 gtacccggag caggctctcc gcggcgtggg attgccacca gcgcgcggcg cggtccggga
     4381 cgaagaggag aagcgcaacc agatcttccg cggcctgggt ggggacgcag gacacgcggc
     4441 ttttgagcgt ctctgccgcg attcccccgg tcgacgccat ctggaagcgg atcgcttccg
     4501 cggtgagctt cctccgcaag agggcgacgc cgtctatccg aagcaccgcc acgacaaagc
     4561 ggcccggctg gtccgggatg acgttccttc gacgaagctc ctcctccagg tcgccgggat
     4621 ccaacgccga gggaccggtg agaaggtccc gaaggagcgc cgacaccacc atgcgatcgt
     4681 tgtccgcggc gtgctcggcg gcgctgaacg cgtccatcgc gcgcgccagg atgccggaga
     4741 cgtactcgag ctcgtttcgt ctgggctcgg gggacgcgga gacgcgggac cgtatcccgt
     4801 cgaatagagc gttgagggga acataaagcc gcagagagag cacaagaccg aagcccagga
     4861 ccgcgatgag aatgcctccg ctggccgcca gcagaatccc tctcagaccc gaaattccgg
     4921 ccgtgtaccg tgcgtaatcg gtgagggaga tggcggtgta gtgatcgttc gcggccctgg
     4981 cgaagctgac cgcgtaacgg cggcccttcg cttcggtcag gaagaagccg ctcgaccgcg
     5041 gatctgccgc gatgcgcctc atgtagggtt cgtccgcgat actggtaccg aagcccgcgg
     5101 cgttcgagcc gaggagcacc gttgcggagt cgtccacgac gacgatggtc tgcggcgcct
     5161 ccgcggcatc cgccccgaga tcctttcgga tggcgtcggc gtccaggttt accaccacgg
     5221 ccgaatccac gtaattggga gagttggact cgttgatcgc cagcgtgagc agggacacca
     5281 gaccgccgtt cgcccgctgc acgagcctgg gcttcggcac caggaccttt ggcatgcgag
     5341 ccagactcag gatggtctgc gggggttcac ccgtctcccc gctctgggac gtgctcagaa
     5401 gcaccgtgtc ccttccacag agagcgaccg aatcgatgta agggtccgaa gccatgagat
     5461 tctgaacgaa ggccgtgatg cgcacgaggt tcagcgggtc cttgccgtcc gtgtacatca
     5521 gggagacgac gtccgggtct ttgaagacaa ggaaagcgat gtcggctgcc tgggtgaagg
     5581 ttcgatccag ccggttcgcc gtttccgcca gggcaagttg ggtcaccgcg gcgagatccc
     5641 gcatgctccg tcggtcatag gcagcgaaca gaatcccgga taccaggacc accgagacca
     5701 cggaggcggc cagaaagctc gagatgagct tcaccaggag ccgctcacgc cggatgagct
     5761 ccagaaagcc ggatagcagc atggtagagc cccgagtata caccaggatc tctcccgcag
     5821 gcaccgccgc gggtgaaatc gaacggatgt gaaaaaaacg tacgtgcccc cgcaggggac
     5881 ttaggacgtt tcttaagtct tttgggcttt gcagccttcc atcggcactg atacgacggc
     5941 ggcaggagac ggtcgtcgcc gtctccgcgg tccgtgaaga ccgcaacgat gcttttccaa
     6001 gggggtaccg gatgaagaaa ggattggctg tggcattggg tggcctgatg ctcctgggag
     6061 cgaccctggc gtttggagcg ccggtcacgc ttcgctacgc gctatgggac ggcgtccaga
     6121 ggcctgccat ggaggcggcg atccagcagt tccagaagga caacccggac atcgtcgtga
     6181 ccacggagct caccgagtgg ggcgactact ggaccaagat ccagaccggg gcggcagccg
     6241 gaacggttcc ggacgtgttc tggggtcacg tggcctactt cgccagcctg gcggacaagg
     6301 gagggctcct ggatctggcg ccgtacatcg cccgggacaa ggtggacctg tcgatctact
     6361 atcccggtct cgtcgacaac tggaagtacg gcgggaagca gtatggcctg gccaaggact
     6421 gggacacgat ctgctacgcc tacaacaagg aaatgaccgc caaggccggc gtggatccca
     6481 cccggatgac ctggaatcca agcgacggcg ggacgttcct gctggcgctc cagaagctca
     6541 cgatcgacaa gaatggaaag aacgccgccc aggccggctt tgatcccaac aacgtcgtgc
     6601 agtacggcat cgccggcatc aacgcggggg agatccagac cggctggctt ccgttcgcct
     6661 ggggcaacgg cgctcccggc agcgtcgtgc tggaccgccc cttcgggacc cggttcgtca
     6721 tggacaaccc ggcgacggtg gccgccctcc agttctacgc ggacctcgtg aaccgatacc
     6781 atgtcgctcc gagcgcctcc ggcaaccaga gcttcgtggg cggcgcgcag gacctcttcc
     6841 tggccaagaa ggtggccatg taccccgccg gtccctggac cctttccggc acccgcgccg
     6901 gggcgacgtt cgcctgggac gtggcgcctc ttccggcggg ccccgccggc cgcgtgaccc
     6961 cgttcaacgg ccttgcccac gtcgtttctt ccacgacgaa gtccagggac gccgcgtgga
     7021 agctggcgag cttcatggac gggcccaagg gagagaacat catcgcctcg ttcgcggtgg
     7081 tcttcccctc cgtcaagagc gcgctgtcca cgtaccagaa ggcattcgaa ggcaaggctc
     7141 cggcgagcat ccacttctac acggatgagg cggccggcaa gacggggccg tatccgcagc
     7201 acctgaagtg gggccagatc tgggacgtcg tccagcgcca gttcgactcc agcttctccg
     7261 gcggcatgtc ggtccagcag gcggtcgccg cggccaaggc cgacgtcgac cctctgctcc
     7321 agtagatccg ttcgcgtcgg gtccggccgc ccgctctggg gtcggacctg accctctagg
     7381 aaggaggccg gggtcatgac tcgagctgcg cagcgaacgg cgcgatccag gcgcttgagc
     7441 gcgtcccgat tctcggaggc gctcgccggc tacctgttca tcctgccgaa cttcgcgggc
     7501 tttctcgtgt ttgccgtggg acccatcgtg gcatcgttcg tccttgcctt cagccggt
//
LOCUS       DASRZS010000577        10366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4172679, whole genome shotgun sequence.
ACCESSION   DASRZS010000577 DASRZS010000000
VERSION     DASRZS010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10366
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4172679"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFH83_14055"
     CDS             complement(<1..380)
                     /locus_tag="VFH83_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487545.1"
                     /translation="MTGSRRLDLVALSTPGSIEAAVAAAQARLFAEHGLSSSQALPPW
                     IPVCFLPPGQAVTGLLARVSRSAPASLAFRTTTVRWEGGWLYLMLDTGGAWQAARAEA
                     ELGTAGTNPGATAAADPGSQPPPHP"
     gene            complement(377..1681)
                     /locus_tag="VFH83_14060"
     CDS             complement(377..1681)
                     /locus_tag="VFH83_14060"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6487546.1"
                     /translation="MRAQAMLGARPYRLWIIVLPPLVLGVALAVLATAGLLALPLLPL
                     WCACLLLALAEGALLARLLRGWRATESRARQLQAVYDVISKAGASLELQEVLDSITRL
                     TVEVTGVRGCAIKLRDPSADHLTIRSVEGIGGAETIPSLEAEDSLVCVPLRHDGRELG
                     ALCVYGEEGRPVTPATLAFLSSLGNLASLSIEHAEVYQTLKRVDEAKGWFLRKAAHEL
                     ASPLAAVQSIAGNLLGGYLGPLAESQRESVERIRARAAGLAEVAADLLVLAKGRALAV
                     DGPPGTVNLCQSLAEALSFYQASAREHGVELAVTGPQAPCTVAGTAEAIRSVISNLVS
                     NAIKYSGPGQTVDVQLTRADQGTELVVSDRGIGIPASEQQRLFEEFFRAANARAHTES
                     GTGLGLAIVRSVLDAIGGRVTIDSQEGRGTTVRVFFPQGPSA"
     gene            1783..2580
                     /locus_tag="VFH83_14065"
     CDS             1783..2580
                     /locus_tag="VFH83_14065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET6487547.1"
                     /translation="MPPTKDFASIADASQPNAGRIYDFLLGGNHNFEVDRQAAQGILQ
                     LIPVLPKLARLIRWFLGEAVRRLSNEGFQHFIDFASGLPTVDHVHQIAPPGSKIIYSD
                     VDPVTVAYGQEIVKDNPNVRYVVCNVENPIGLLESQDVKDLFGSERKVAIGFNGIAYF
                     LPDEKMAHSLEVLYQWAAPGSKLFMTDAHIPTMGKQAQEIGQIYAKMNQPYYARVLDT
                     IKKLVGPWKVAAPGFLELEEWVEMSPTAGAELKSTFGSSMVGCILSK"
     gene            complement(2585..4345)
                     /locus_tag="VFH83_14070"
     CDS             complement(2585..4345)
                     /locus_tag="VFH83_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014653111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487548.1"
                     /translation="MEKAKNIRGALLRIWGYLRQQRAALLVVVLLVVGTTSVTVVTPY
                     FFGVAIDRYIVPGDFRGLLGLVVLVCGLYAAQAVGLWLQSWVMASLAQDTVRAMRREL
                     FHSLLALPVRFFDRTTRGELMSRLANDAENVNVTLNQGLTLILSSALTLVGTLAMMIA
                     LSPMLTLLSLVVLPASLLITRAVARAARESFREQQESLGDLNGHIEETISGRRVVQAF
                     GRERHTLETFDRHNLRLRSSGTRALIVSGNMGPYMNLVNNIGFAVVAAGGGYLALRHL
                     ATAGLIAAFISYARQFSRPVNEIANQYSQIQSAVAGAERVFDIMDQPPEPADAPQARA
                     LTRVEGRVRFSSVHFGYDANRPVLTDVSLEATPGRTIALVGPTGAGKTTIVNLLTRFY
                     DIDSGTISLDGQDIRLLPRDGLRSALGIVLQDTHLFSETVRENIRYGRLSATDAEVVE
                     AARTANAESFILRLHNGYDTVLSEEGGSLSQGQRQLLSIARAILADPAVLILDEATSS
                     VDTRTELHIQQAMLRLMRGRTSFVIAHRLSTIRDADEILVINGGRIIERGTHPELLER
                     KGFYWRLYSTQFDRTGNAGA"
     gene            complement(4449..6182)
                     /locus_tag="VFH83_14075"
     CDS             complement(4449..6182)
                     /locus_tag="VFH83_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007130371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487549.1"
                     /translation="MLKLFRYLKPYWRDALVAPLLMIVEVTSDLLQPTFLARIVDRGV
                     ANGDLPFVLRTGLEMIGVVLVGALGGIGCTITASRASMRFAADLRRDAYRRIQSLSFA
                     RQDAFSTSSLVTRLTNDVVQMQNLVLMMLRVLVRAPLLCLGGIALAVALNPRLSLVLV
                     VALPLLIGVMLIVVRRGFPLFATVQEKLDRVNAVMQENLSGVRVVKAFVRAPRERERF
                     GAANDALRDSTVRAQRSLALTNPLMMLVMNLSVVAVIWFGGIQVHVLGIGVGQLMAFV
                     NYLIQIMSSFAMVAFMLLSVSRAFASARRLNEVLDAPIDIADAARAGERSIRDGGVVF
                     DRVSFRYEESAGDPVLSEVSLRAEPGETVAILGSTGSGKSTLVGLIPRFHEATAGRVL
                     VDGHDVSSYRLEALRGSIGVVLQDSILFTGTIRENIRWGKEDATEEEIIQAARDAQAH
                     EFISGFPDGYDTVLGQGGVNLSGGQKQRLAIARALVRQPAVLILDDSTSAVDMGTEAR
                     IQAALRSRPRRCTCFVIAQRISTVMDADRIVVLENGRVDAMGTHQELLERSAVYREIC
                     ASQLGEEAAGG"
     gene            6288..7073
                     /locus_tag="VFH83_14080"
     CDS             6288..7073
                     /locus_tag="VFH83_14080"
                     /inference="COORDINATES: protein motif:HMM:NF024626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HET6487550.1"
                     /translation="MQRKVRKIASGIAERIGKVDGVLAILLGEAADIEAIDPYFTIDL
                     DVFVDGQIPTVETRRGLLGDAEAFETSPVSAVDRFLIDKLPASLHFIRTDDVDRMLLR
                     IAERSWVFHEPGTNTLYRLERGEVMFSREGWLEGARAALAHVPPQFWWQARLRAFAMA
                     ERALTDLGAAAHRSDDLFFTVSSARLIRSVASFLFAVNRQFEPSDRMLAERIQALPKK
                     PDELSGRLDSFMRPEGKLSKMARREIAELLVRSLLPLAVEESA"
     gene            7070..8758
                     /locus_tag="VFH83_14085"
     CDS             7070..8758
                     /locus_tag="VFH83_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487551.1"
                     /translation="MRRAPRGHRIPSGTRASVRAGAAAAVLLLLIACQSRFESFSFIT
                     ADGTSIEIFEKPGQRPAAEEQFQPTSANPSSPIYSLRRTVPIQQDGLAFAISYTSSIP
                     ECTLILYSDSRTVLRKASLPASSGTRLRFLVPLHRGDRIWGYRLSASAADPGGVESLQ
                     LLGAGTSAFVHGFAVEEGDLAVDGSVGVLESSPAGVQARLTSATRQDLSRGWWLISLE
                     PGAVETDHAGRVDLVSADGARTGFTVDAGFLAGSHRIDFARGSVPFTPRDVQTSFPIR
                     SLRLAFLPSDAPIPADPGTILTWDRSQWRRPEFELFSWDRYPGVLIFDTASYDVQDDL
                     FNRLAFFVEKAGHVGAIPSPASLAGLHGYNAHDYRAPDLARFFSAVQESGVQLTAGET
                     RLRDILVNAGEIVSSGSGYAAGTGSVLSISRESSPILRFLLLTHESFHGILFSLPEFQ
                     HDVEQIWNDLSPVEQEVWRAFLASRGYDATDHYLVVNEFQAYLFQQDRAAVPGFQALT
                     LSRLRATSPRAASLVQELLAQHPDSFLRSFDSLDAALTSLGGPSGGKSLEILRE"
     gene            8835..>10366
                     /locus_tag="VFH83_14090"
     CDS             8835..>10366
                     /locus_tag="VFH83_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487552.1"
                     /translation="MEIQRHFTRLGESPYEGIAFESRVSEIKNPDGKVIFRQENVTVP
                     QSWSQIATDILAQKYFRKRGVPQTDGKSGGENDARQVFHRLAFTWMDWGKRHGYFASD
                     ADAQTFYDEMCFMLAHQMGAPNSPQWFNTGLFAVYGLKGPAQGHYFVNPKTMEVEKAT
                     SAYERPQPHACQPWHASVSTPFGPVAIGSIVDGGLVGLEVFDGTDEGRGTTRVVAVKE
                     NGVKPVFRVILENGTTVEATGDHLVLASRERGTAPEWVRMDALEAGMRLQVSTVSEAR
                     EVAVANRFPGKHLPAIREEPVTRLELAGLMRVYDIQTESGQYLSNNVIVHNCFILSVE
                     DDLVNDGGIMDLMTREARLFKYGSGTGTNYSKLRSSSEPLSGGGVSSGLLSFLKVSDR
                     SASSIKSGGTTRRAARMVTLDADHPDVFKYIDWKVQEERKVASMVTGSRILRRHTKRL
                     LQACKPPAASNGNAAAATLDPRKNKELARAIQEALQEEVPSSWIYQVIQLAGQGIFEI
                     EP"
BASE COUNT         1775 a         3519 c         3452 g         1620 t
ORIGIN      
        1 gggtgcggcg gcggctgcga acccgggtcc gcggccgccg tcgcacccgg gttcgtgccc
       61 gctgttccca gctccgcttc cgcgcgcgcc gcctgccacg cgccgcccgt gtccagcatc
      121 aggtaaagcc aaccgccttc ccatcgcacg gtggtcgtcc gaaaggccag agaggccgga
      181 gccgagcgcg agacccgcgc caggagcccg gtgaccgcct gcccaggcgg aaggaagcag
      241 acggggatcc agggcggcag cgcctgggag gaagacaggc cgtgctccgc gaagagccgc
      301 gcctgcgccg cggccaccgc ggcctcgatc gaccccggcg tggagagcgc cacgaggtcg
      361 agccgccgag accctgtcac gcgctggggc cctgcgggaa gaacacccgc accgtggtgc
      421 ccctcccctc ctggctgtcg atggtcaccc gtccgccgat ggcatccagc acggagcgga
      481 cgatggcgag accgagcccc gtaccggact ccgtgtgagc ccgcgcgttg gcggctcgga
      541 agaactcctc gaagagccgc tgctgctcgg aagcgggaat gccgatccca cggtcggaaa
      601 cgaccagctc ggtcccctgg tccgcgcggg tgagctggac atccaccgtc tgacccgggc
      661 cggagtactt gatggcgttg gagaccaggt tggagatcac cgagcggatt gcctccgccg
      721 tcccggccac ggtgcagggt gcctgcggcc cggtgaccgc cagctccacg ccgtgctccc
      781 gcgccgaggc ctggtagaag gacaacgcct cagccaggga ctggcagagg ttgaccgtgc
      841 cgggcggccc gtctaccgcg agcgcgcggc ctttggccag gaccaggagg tccgctgcca
      901 cttccgccag gcccgcggcg cgcgcgcgga tgcgctccac gctctcccgc tggctctcgg
      961 cgagcggccc caggtagccg cccagcaggt tgcccgcgat cgactgcacc gcggcaagcg
     1021 gggatgccag ctcgtgagcc gccttgcgga ggaaccagcc cttggcctca tcgacgcgct
     1081 tcagcgtctg gtacacctcg gcgtgctcga tggacaggga ggcgaggttc cccaggctcg
     1141 agagaaaggc gagcgttgcc ggcgtcacgg gcctcccctc ctcgccatag acgcacaggg
     1201 ctccgagctc gcgcccgtcg tgtcggagcg gaacgcaaac cagggaatct tcggcctcca
     1261 ggctcggaat agtctcggcc ccgccgatcc cttcgaccga acggatggtg aggtggtctg
     1321 ccgatggatc ccgcagcttg atggcgcagc ctcggacgcc ggtcacttcc acggtaaggc
     1381 gcgtgatcga gtccaggacc tcctggagct ccagggacgc ccccgccttg ctgatcacgt
     1441 cgtagacggc ctggagctgc cgggcccggg actccgtggc ccgccacccg cgaagcaggc
     1501 gcgccaggag cgcgccctcg gcgagcgcca ggaggaggca cgcgcaccag aggggaagca
     1561 gcgggagggc aagcagccct gcggtagcga ggacggcaag cgcaactccc aggacaaggg
     1621 gaggcaggac gatgatccag agccggtaag gtctggcgcc gagcatcgcc tgcgcgcgca
     1681 tggtgcgccg atggtaccac gacgggcagg ccgcaacaag ctcggaccgc gttcttgcaa
     1741 aaacaggggt ttccaccata ataggagctt gaggagtcat gcatgccacc cacgaaggac
     1801 tttgccagta tcgccgacgc gagccagccg aacgccggcc gtatctacga tttcctgctc
     1861 ggcggtaacc acaatttcga agtcgatcgc caggcggcgc agggcatcct gcagctcatc
     1921 cccgtcctgc cgaagcttgc caggctcatc cgctggttcc tcggggaggc ggtgcgaaga
     1981 ctctccaacg aaggattcca gcacttcatc gatttcgcct ccggcctgcc tacggtagac
     2041 catgtccacc agatcgcgcc cccgggctcg aagatcatct actccgacgt ggacccggtt
     2101 accgtggcgt acgggcagga gatcgtcaaa gacaatccca acgtgcgcta cgtggtctgc
     2161 aacgtggaga acccgattgg tctgctggaa tcccaggacg tcaaggatct tttcggctcg
     2221 gagcgcaagg tggccatcgg cttcaacggg atcgcctact tccttccgga cgagaagatg
     2281 gctcactccc tggaggtcct ctaccagtgg gcggcgcccg gctccaagct tttcatgacg
     2341 gatgcccata tcccgaccat gggaaagcag gcccaggaga tcggccagat ctacgccaag
     2401 atgaaccagc cctactacgc ccgcgtgctg gacaccatca agaagctggt cggcccctgg
     2461 aaggtggcgg cgcccggctt cctcgagctg gaggaatggg tggagatgtc cccgaccgcc
     2521 ggcgcggagc tcaaaagcac cttcgggagc tccatggtgg ggtgcatcct ctcgaagtag
     2581 atcctcacgc gccagcgttt cccgtccggt cgaactgggt cgagtacagc ctccagtaga
     2641 aacccttccg ctccagaagc tccgggtgcg tgccgcgctc gatgatccga ccgccgttga
     2701 tcaccaggat ctcatccgcg tcccggatgg tgctcagccg atgcgcgatc acgaagctcg
     2761 tccgaccccg catgaggcga agcatggcct gctggatgtg cagctccgtt cgcgtgtcca
     2821 cgctgctggt ggcctcgtcc aggatgagga ccgcggggtc cgccaggatg gcgcgggcga
     2881 tggacaggag ctgccgctgg ccctggctca ggctgccgcc ttcctcagag agaaccgtgt
     2941 cgtagccgtt gtgaagccgc aagatgaagc tctccgcgtt cgcggtgcgc gccgcctcca
     3001 ccacctccgc gtccgtggcg gagagcctgc cgtagcggat gttttctcgc acggtctctg
     3061 agaagaggtg cgtgtcctgg agcacgatgc ccagcgcgga gcgcagcccg tcccggggaa
     3121 ggaggcggat gtcctggccg tccagcgaga tggtgccgga gtcgatgtcg tagaaccggg
     3181 taagcaggtt gacgatggtg gtcttcccgg ctcccgttgg gcccaccagc gcgatggtgc
     3241 gccccggggt ggcctccagc gagacgtcgg tgagaacggg tcgattggcg tcgtacccga
     3301 agtgcacgga agagaaccgc acgcgcccct ccacgcgcgt gagggcgcgc gcctggggag
     3361 cgtcggccgg ctcgggaggc tggtccatga tgtcgaagac ccgttcggcg ccggccaccg
     3421 cggactggat ctgggagtac tggttggcga tctcgttcac gggtcgagag aactgacggg
     3481 cgtaactgat gaacgcggcg atcagccccg cggtggccag atggcggagc gccagatagc
     3541 ctccgccggc ggcgaccacc gcgaagccga tgttgttgac caggttcatg tagggcccca
     3601 tgttgccgga gacgatgagg gcccgggtgc ccgagctccg caggcggagg ttgtgccggt
     3661 caaaggtctc cagggtgtgc cgctcccgcc cgaaggcctg gaccacccga cgcccgctga
     3721 tggtctcctc gatgtggccg ttgaggtccc ccaggctctc ctgctgctcg cgaaaggact
     3781 cccgcgccgc ccgagccacc gcgcgggtga tcagcaggct cgcggggagc accacgaggc
     3841 tgagcagcgt cagcatcggg ctgagggcga tcatcatcgc cagcgtgccc accaacgtca
     3901 gggcgctgga gaggatgagc gtgagcccct ggttcaaggt gacattcacg ttctccgcgt
     3961 cgttggcgag ccggctcatg agctcgccgc gcgtggtgcg atcgaaaaag cggaccggaa
     4021 gcgccagcag ggaatggaag agctcgcgcc gcattgctct cacggtatcc tgcgccaggc
     4081 tggccatgac ccagctctga agccacaacc ccaccgcctg ggccgcatag agcccgcaga
     4141 cgaggacgac cagacccagc aggccccgga aatcgccggg cacgatatag cgatcgatcg
     4201 ccaccccgaa gaagtagggt gtgacgacgg tgacgctggt ggtcccaacg accaggagga
     4261 ccaccaccag gagcgcggcc cgctgctggc gcaggtaacc ccagatccga aggagcgcgc
     4321 cacggatgtt cttcgccttc tccaccggac cgaatcgctg cccgggtccg gcccccggcc
     4381 ggcccggggg accgaagggg agtggaggct gcgcgtcgcc agatcggcgc ctctcggcag
     4441 gccgcgcgtc agccaccggc cgcctcctcg ccgagctgcg aggcgcagat ctcccgatag
     4501 acggcgcttc tctccagcag ctcctgatgg gttcccatgg catccacccg cccgttttcc
     4561 aggaccacga tccggtccgc gtccatcacg gtgctgatcc gctgggcaat gacgaagcag
     4621 gtgcaccggc ggggacgaga gcgcagcgcc gcctggatgc gcgcctcggt gcccatgtcc
     4681 acggcgctgg tgctgtcgtc caggatcagg acggccggct ggcggaccag ggctcgggca
     4741 atggccagcc tctgcttctg cccaccggag aggttcacgc caccctgccc aagaaccgtg
     4801 tcgtagccgt ccgggaagcc gctgatgaac tcgtgcgcct gcgcgtcccg tgccgcctga
     4861 atgatctcct cctcggtggc atcctccttg ccccagcgga tgttctcacg gatggtgccg
     4921 gtgaagagga tcgagtcctg aagcaccacg ccgatgctcc cgcgcagcgc ctccagccga
     4981 taggaggaga cgtcgtggcc gtccaccagc acccgtcccg ccgtggcttc gtggaagcgg
     5041 gggatcaggc ctaccagcgt ggacttgcca gagccggtgg agccaaggat ggcaaccgtc
     5101 tctccgggct cggcgcggag gctcacctcc gatagcaccg ggtcccccgc ggactcctcg
     5161 tatcggaagc tcacccgatc gaacaccacg ccgccgtccc ggatcgatct ctcccccgcg
     5221 cgggcggcgt ccgcaatgtc gatgggcgca tccagcacct cgttcagccg ccgcgccgag
     5281 gcgaacgcgc gcgagacgga gaggagcatg aaagcgacca tcgcaaaaga ggacatgatc
     5341 tggatgaggt agttgacgaa ggccatgagc tgccccaccc cgatccccaa gacgtgcacc
     5401 tggatcccgc cgaaccagat cacggcaacc acgctgaggt tcatgacgag catcatgaga
     5461 gggttggtga gggccaggga ccgctgcgcg cggaccgtgg aatcgcgaag agcgtcgttt
     5521 gccgccccga agcgctcccg ctcgcgaggc gcgcgcacga acgccttcac cacgcgcacc
     5581 ccggacaggt tctcctgcat caccgcgttc acgcggtcca gcttctcctg gaccgtcgcg
     5641 aacaggggga acccgcggcg caccacgatg agcatcacgc cgatgaggag cgggagcgcg
     5701 accaccagga ccagggacag gcggggattg agcgcgacgg caagggcgat cccgcccaga
     5761 cacaaaagcg gcgcgcggac caggacgcgc agcatcatga ggacgaggtt ctgcatctgc
     5821 acgacgtcgt tcgtgagcct cgtcaccagg gaggaggtgg agaacgcgtc ctggcgggca
     5881 aaggacaggg actggatcct gcgataggcg tcccggcgca gatccgcggc gaaccgcatg
     5941 gaggcccggc tggcggtgat ggtgcacccg attcccccga gcgctcccac caggacgacg
     6001 ccgatcatct ccagcccggt cctcaggacg aacgggaggt ccccgttggc cacgccccgg
     6061 tccacgatcc gggcgaggaa cgtgggctgc agcaggtcgg aagtaacctc gacgatcatg
     6121 aggaggggag ccaccagcgc gtccctccag tagggcttca ggtagcggaa gagcttcagc
     6181 atgggatcgg tcccgccagg atagggtccg cgcgcggccc ggtcaagccg ggaaaaccct
     6241 acgtggcgcg agttgcgcgc agccgtaagc cctgctacta tggcctcatg caaaggaagg
     6301 tgcgaaagat cgcctcgggg atcgcggagc gcatcggcaa ggtcgacggg gtactggcca
     6361 tcctgctggg tgaggcggcg gacatcgagg ccatcgaccc ctacttcacg atcgatctcg
     6421 atgtgttcgt ggacggccag atccccacgg tggagacccg ccgcgggctc ctgggggacg
     6481 cggaggcctt cgagacctcc ccggtgtccg ccgtggaccg tttcctgatc gacaagctgc
     6541 cggcaagcct gcacttcatc cgcaccgacg acgtggaccg catgctcctg cggatcgccg
     6601 aacggagctg ggtcttccac gagcccggca cgaacaccct ctaccggctg gagcgcgggg
     6661 aggtgatgtt ctcccgggaa ggctggctgg agggagcccg cgccgcgctg gcccacgtcc
     6721 cgccgcagtt ctggtggcag gcgcggcttc gcgccttcgc tatggcggag cgcgcgctga
     6781 cggacctggg cgccgccgcc catcgctccg acgacctgtt cttcacggtt tcctccgcgc
     6841 gtctgatccg cagcgtggcg agcttcctct tcgcggtgaa ccgtcagttc gagccttcgg
     6901 accggatgct ggcggagcgc atccaggcgc ttcccaagaa gccggacgag ctctccggcc
     6961 ggctggatag cttcatgcgg cccgagggca agctttccaa gatggcccgg cgggaaatcg
     7021 cggagctcct ggtccgaagc ctcctgcccc tcgcggtcga ggagtccgcg tgaggagggc
     7081 cccccggggc caccgcattc cctcagggac cagagcctcc gtccgcgccg gcgcggcagc
     7141 cgcggtcctg ctcctgctca tcgcgtgcca gagccggttc gagagcttct ccttcatcac
     7201 cgcggacgga acgtcgatcg agatcttcga gaaacccgga caacggccgg ccgcggagga
     7261 acagtttcag ccgacctcgg ccaatcccag ctcgccgatc tactctctgc gccggacggt
     7321 gcccatccag caggacgggc tggccttcgc catctcctac accagcagca tccccgagtg
     7381 tacgctcatc ctgtactccg actcccgcac cgtgcttcga aaggcctcac tgccggcttc
     7441 cagcggaacc cggctccgat tcctggtgcc tctgcaccgg ggggatcgga tctggggata
     7501 ccggctgtcc gcgagcgccg cggatcccgg aggggtggag tccctccagc tcctgggagc
     7561 cggcacatcg gcctttgtcc acggcttcgc ggtggaggag ggcgatcttg cggtggacgg
     7621 cagcgtaggg gtcctggagt catccccggc cggagtgcag gcgcggctga cttcggcaac
     7681 gcgtcaggac ctctcccgcg ggtggtggct gatcagcctg gagcccggcg ccgtggagac
     7741 cgaccacgcg ggccgcgtgg acctcgtctc ggcggacggc gcgcggaccg gtttcaccgt
     7801 ggatgccgga ttcctggccg ggagccatcg catcgacttt gcccgaggga gcgtaccgtt
     7861 cacgccccgg gacgttcaga ccagcttccc gatccgaagc ctccgtctgg ccttcctccc
     7921 gtcggacgcg cccatccccg cggatcccgg gaccatcctc acctgggacc gctcccagtg
     7981 gcgccgaccg gagttcgagc tcttctcttg ggaccgatat ccgggcgtcc tcatcttcga
     8041 caccgccagc tacgacgtcc aggatgacct cttcaaccgc cttgccttct tcgtcgagaa
     8101 ggcggggcac gtgggtgcca ttccttcccc cgcctccctc gcggggctgc acgggtacaa
     8161 cgcccacgac tatcgggcgc cggacctggc gcgcttcttc tcggccgtcc aggaaagcgg
     8221 tgtgcagctc accgcggggg agacgcggct tcgggacatc ctggtgaacg cgggagagat
     8281 cgtgtccagc ggctcgggct acgctgccgg cacgggctcc gtgctgtcca tatcgcggga
     8341 atcgtccccg atcctccgat tcctcctcct cactcacgaa agctttcacg gcatcctgtt
     8401 ctctctgccg gagttccagc atgacgtgga acagatctgg aacgatctca gccccgtgga
     8461 gcaggaagtg tggcgggcgt tcctggcgtc ccgcgggtac gacgcaacgg atcactacct
     8521 ggtggtcaac gagttccagg cgtatctctt ccagcaggat cgagccgccg tgccggggtt
     8581 ccaggcgctc acgctttccc gcttgagggc aacgtcgccg cgcgccgcga gcctggtcca
     8641 ggaattgctt gcgcagcatc ctgactcatt cctgcgctcc tttgactcgc tggacgccgc
     8701 tcttacgtcg ctcggcggtc catccggagg aaagtccctg gagattcttc gcgagtagtg
     8761 cgtttgctat tgccttctgc cggaagtttc cgttaccata gaatttcgcc cagtcaatct
     8821 tcgggagggg gaccatggaa atccagcggc acttcacgcg cttgggggag agcccctatg
     8881 aaggtatagc cttcgagtct cgcgtctcag agatcaagaa cccggacgga aaagtcatct
     8941 tccgtcagga gaacgtcacc gtaccccaat cgtggtccca gatcgcgacg gatatcctcg
     9001 ctcagaagta cttccgaaag cgcggcgttc ctcagaccga cggcaagagc ggcggagaga
     9061 acgacgcccg ccaggtcttc caccggctgg cgttcacgtg gatggattgg ggcaagcgtc
     9121 acggctactt cgccagtgac gcggatgcgc agaccttcta cgacgagatg tgcttcatgc
     9181 tggcgcacca gatgggcgcg cccaactcgc cccagtggtt caacacgggc ctgttcgccg
     9241 tctacggtct gaagggtccg gcccagggac actacttcgt caacccgaag accatggagg
     9301 tggagaaggc caccagcgcc tacgagcgcc cgcagccgca cgcgtgccag ccgtggcatg
     9361 catcggtgtc caccccgttc ggccccgttg ccatcggatc gatcgtggac ggcgggctgg
     9421 tgggcctcga agtcttcgac gggacggatg agggccgcgg gaccacccgt gtggtcgcgg
     9481 tgaaggagaa cggggtcaag cccgtgttcc gcgtcattct ggagaacggt acgaccgtcg
     9541 aagcaacggg agaccacctc gtgctggcgt ctcgggagcg gggcaccgca ccggaatggg
     9601 tccgaatgga tgcgctcgaa gccgggatgc gcctgcaggt ttctacggtc agcgaagctc
     9661 gcgaggtggc cgtcgcaaac cgcttcccgg ggaagcatct accggcgatc cgagaagagc
     9721 cggtgacaag acttgagctc gccgggctga tgcgggtcta cgacattcag acggaaagcg
     9781 ggcagtatct ttcgaacaac gtcatcgtcc acaattgttt catcctctcc gtggaagacg
     9841 acctggtgaa cgacggcggg atcatggacc tcatgacccg tgaggcgcgc ctcttcaagt
     9901 acggctcggg gaccgggacc aactactcca agctgcggtc ctccagcgag ccgctctccg
     9961 gcggcggcgt ctcctcgggc ctgctgtcgt tcctcaaggt gagcgaccgc tccgcctcct
    10021 ccatcaagag cggtgggacc acgcggcggg cggcccgcat ggtcacgctg gacgccgatc
    10081 acccggacgt cttcaagtac atcgactgga aggtgcagga ggagcgcaag gtcgcctcca
    10141 tggtgacggg gagccggatc ctgcggcgtc acaccaaacg cctcctccag gcgtgcaagc
    10201 cgccggccgc cagcaacggg aacgccgcgg cggccacgct ggaccctcgc aagaacaagg
    10261 agctggcgcg cgccatccag gaggccctgc aggaagaggt gccttcttcc tggatctacc
    10321 aggtgatcca gctcgccggc caggggatct tcgagatcga gccgga
//
LOCUS       DASRZS010000578         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3020375, whole genome shotgun sequence.
ACCESSION   DASRZS010000578 DASRZS010000000
VERSION     DASRZS010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3020375"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VFH83_14095"
     CDS             complement(<1..541)
                     /locus_tag="VFH83_14095"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET6487553.1"
                     /translation="MKAVPRGPRTRGRPARGSSPTLSRDRIVHAALGIVDKNGLAALS
                     MRRLGTALGVDPMAIYYHLPGKAALYDAIVEAVMAEMDLSAPIRGSPLEQLKRMAGAY
                     KDALLEHPNALPVVAARPVRTPDSLRPVERMLAVLKGLGFSAQTALACVDVCAHFVIG
                     WAMTYVAHLQDSEMHRHSGI"
     gene            complement(538..1305)
                     /locus_tag="VFH83_14100"
     CDS             complement(538..1305)
                     /locus_tag="VFH83_14100"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487554.1"
                     /translation="MLRRSFSLACNSIKLYLADPAPMVIYLIMPVGLMAFLVPASQGL
                     LLSQGYAHASGAEQAVPGMTVMFSFFVVGVIGVQFFREQGWGTWDRLRIASGALEILV
                     GKIVPGLLLLLCQFLIVFASGGLLFRLHVAGSLTGLLLIALGFALSVLSMTMALIAWC
                     KTLDQLNVIASVLSIVLSGLGGSFAPVNVLPAWARILAKASPAYWALGGMRAIILDGE
                     GIGTALSAIAVLAAFTAAFGVLTAVRLNIREAKVSET"
     gene            complement(1292..2227)
                     /locus_tag="VFH83_14105"
     CDS             complement(1292..2227)
                     /locus_tag="VFH83_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487555.1"
                     /translation="MLSITGLRKTFGPTIALDGVDLEVAPGEICGLIGPNGAGKTTLV
                     SIATGLREADSGRLRIDGHDATADRRAIRGIVGCAPQDLGMYPTLSVRTNLEFFGRWN
                     GVARKDLPARINEVCRRLDLESLLQRRAGDLSGGQKRRVHTALALLHRPRLLFLDEPT
                     VGADVAARRKLLEAVRLMASEGCAVVYATHYLAEIESLGASVAVLEAGRIRERGALTD
                     VVARYGQARAELEFQGSAPALEGWRRSGARLWTTTPDPASATARAIARLNGEASRLRG
                     VRIVPSSLESAYLAITGHGTDPAAEETKEDADAQA"
BASE COUNT          446 a          780 c          792 g          386 t
ORIGIN      
        1 agatgccgct atggcggtgc atttcggagt cctgcaggtg cgccacatag gtcatcgccc
       61 atccgatgac gaagtgggcg cacacgtcca cgcaggcgag ggcagtttgc gcggagaatc
      121 ccaacccctt gagcacggca agcatccgct cgaccggccg gagggagtcc ggcgtgcgga
      181 ccgggcgggc ggccaccacg gggagggcgt tggggtgctc caggagcgca tccttgtagg
      241 cgccggccat ccttttcagt tgctccagag gcgatccccg gatcggggcg gacagatcca
      301 tctccgccat gaccgcttcg acgatcgcat cgtagagagc ggccttgccg gggaggtggt
      361 agtagatggc catgggatcc accccgagcg ctgtacccag ccgccgcatc gagagcgcag
      421 cgagcccgtt cttgtccacg atcccgagcg ctgcgtggac gatgcggtcc cgcgagaggg
      481 tcgggctcga cccccgtgcc ggtctgcctc gtgtccttgg gccgcgggga acggctttca
      541 cgtttctgat accttggcct cccggatgtt gagccgtacg gcggtgagga cgccaaaggc
      601 ggccgtgaag gctgccagca ccgcgattgc agaaagggcg gtcccgatcc cctcaccgtc
      661 caggatgata gcacgcatgc ctcccagggc ccagtaggcc ggggacgctt tggccaggat
      721 acgcgcccat gcgggaagca catttacggg agcgaacgat ccgcccaggc ccgagagcac
      781 aatggagagg acactggcga tgacgtttag ctgatccagg gtcttgcacc aggcgatcag
      841 agccatcgtc atcgagagga cgctcagcgc gaagccgagc gcgatgagga gaagccccgt
      901 cagagaaccc gccacgtgca accggaagag cagccctccc gaagcgaaga cgatcagaaa
      961 ctggcatagc aggagcaaaa gcccgggcac gatcttgccg acaaggatct ccagggcgcc
     1021 cgaagcaatc cgaagccggt cccacgtccc ccaaccttgt tcccggaaga actgcacgcc
     1081 gatgacgcca accacgaaga agctgaacat caccgtcatg cccggcaccg cctgctcggc
     1141 tccactggcg tgcgcgtagc cctgggacag aagcagcccc tggcttgccg ggacaaggaa
     1201 cgccatgagg ccgaccggca tgatgagata gatcaccatc ggggccgggt cggcaagata
     1261 gagcttgatc gagttgcacg cgagcgagaa gctacgcctg agcatcggca tcctccttcg
     1321 tttcttccgc cgcaggatcc gttccatgac ccgtgatggc gaggtaggca ctctccaggc
     1381 tcgaaggcac gatccgaacg ccgcgcaggc gggacgcctc gccgttcaga cgtgcgatcg
     1441 cgcgggcggt tgccgaagcc gggtccggtg tcgtggtcca cagccgggcg ccactcctcc
     1501 gccagccctc cagcgcaggt gcgctgccct ggaactccag ctcggcgcgc gcctggccgt
     1561 agcgtgcgac cacatccgtg agggcacccc gttcccggat tcttcccgct tccagcacgg
     1621 ccaccgacgc tcccaacgac tcgatctccg cgaggtagtg tgtggcgtag acgacggcgc
     1681 aaccctcaga ggccatgagt ctcaccgctt ccaggagctt gcggcgtgcc gcgacgtcag
     1741 cccctaccgt cggttcgtcc aaaaagagga gccgcggtcg atgaagcagg gcaagcgccg
     1801 tgtgaacccg tctcttctgg ccaccggaga gatcccctgc gcggcgctgg agaaggctct
     1861 cgaggtcaag gcgacggcat acctcgttga tgcgcgcggg gagatccttg cgcgccacgc
     1921 cgttccagcg accgaagaac tccaggttcg tccgcacgct gagggtcggg tacatcccga
     1981 gatcctgagg cgcgcacccg acgatccccc ggatcgctct acgatcggcg gtggcgtcgt
     2041 ggccgtcgat gcgcaggcgc cccgagtcag cctctcggag gcccgtggcg attgagacca
     2101 gggtggtctt gcccgcgccg tttggcccga tgagcccgca gatctcgccc ggggccacct
     2161 ccagatccac cccgtcaaga gcgatcgtgg gaccgaaggt ctttctgaga cccgtgatgg
     2221 agagcatgcg tagcctcctt tatttctaca tagtagaata taactctacg gtgtagaaat
     2281 tgcaaggggg ccgggatgga tcttgagagg atcgatggga cggggaggcc ctcgacttgg
     2341 tcgtttgctg gggacgccct ggagagcgcg gtgtagtgcg ggtccatgct gctggagtcc
     2401 ttct
//
LOCUS       DASRZS010000579         5208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4936665, whole genome shotgun sequence.
ACCESSION   DASRZS010000579 DASRZS010000000
VERSION     DASRZS010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4936665"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFH83_14110"
     CDS             <1..495
                     /locus_tag="VFH83_14110"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6487556.1"
                     /translation="SMALLHEKLYQSKSMAGVEFSAYVRDLTRNLFQSYGVNSGASEL
                     ELQVSDVTLGMDTAVPCGLIINELVSNSLKHAFPPRQPGKVSISLKPDHAPKVDAGHP
                     PGEKWYMLTVADKGRGFPDGVDFRNTDSLGLKLVCTLTEQLFGTIDLNRNGGTEFTIR
                     FREL"
     gene            629..1243
                     /locus_tag="VFH83_14115"
     CDS             629..1243
                     /locus_tag="VFH83_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487557.1"
                     /translation="MNRLNAAGPILAIFSAALLTMGCATITPANTQKPDVQFTVSVDS
                     IPRGASVYAVNADGTLGALLGLTPYQWRVGLSMARDSSGAPAFGYAAQLFGPPGISLG
                     TVHKGYGFFSADEWSWQNLLISVAVVKEGLPTTWYANRVVARVGQGPNGAYPYPPQDV
                     TVTLPLGAQASALPAQPPETIIVPAPYPWPHRWVYPYRWRRHRP"
     gene            complement(1246..1443)
                     /locus_tag="VFH83_14120"
     CDS             complement(1246..1443)
                     /locus_tag="VFH83_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487558.1"
                     /translation="MSKSASRIIGIVAVVVSVLLGGYGVYWFVLGRIKHGLLFAALFV
                     LVLLFGIVQLRLSAGQKAQGA"
     gene            1499..1945
                     /locus_tag="VFH83_14125"
     CDS             1499..1945
                     /locus_tag="VFH83_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET6487559.1"
                     /translation="MTRSELLAFFRSHINAVEATVSAEGAPQAAVVGIAVTDQFEIVF
                     DSLETTRKARNLSLSPRIALVIGGWIAGDERTLQYEGVADRPSGAELERLKSVYYEVF
                     PTGPSRLSWAGLVYLRARPTWVRYTDFGKSPVERIELNADQLRMLE"
     gene            complement(1947..2699)
                     /locus_tag="VFH83_14130"
     CDS             complement(1947..2699)
                     /locus_tag="VFH83_14130"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HET6487560.1"
                     /translation="MKGLSASVAIGVLTMALVWPLSAQEAALTANDVLVRVDDAVNGA
                     KDQTYAMKLVLLDRFGGEKTQELQMWQKGRDRRLARITAPAIQKGIAFLSLPDGVQYL
                     YLPAFGKAQRITAQLKVASFTGTDFTYEDMEAGRESERWDPRILKQDPEAIVLELTPK
                     PGAVSDYSKLVMTVRTDTFLPVRIEHYDKSGKLWKVLVREKLQRVGGYWVAMESTMED
                     LARQHKTRMIISEMKFDTGIPDSLFTEAAMSR"
     gene            complement(2696..3562)
                     /locus_tag="VFH83_14135"
     CDS             complement(2696..3562)
                     /locus_tag="VFH83_14135"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET6487561.1"
                     /translation="MWLAGKRQPGTAVNADGVAQLLTRLMKDGTAGTAEGTTAALLQA
                     FPTALQQDPAFRGDVSVELLDLSQPTVAIPEKLFKGLPTATQGEPPVISFSVGFTGMP
                     LISWHLDRSILSSQAESLLIALVFIFALLALRLRSARAGALGLVPIILAIVAMFGVMG
                     YAGIPINVATVLVGSIALGIGIDYVIHFSVRFATFYRGETTAVEAVKKTIQTTGLAII
                     INVLAVTMGFVALLFANLLPLRQFGILTAIAMLASGLGAFTLLPALILLVPSAFRGGR
                     KPVRVQKEMLRS"
     gene            complement(3508..>5208)
                     /locus_tag="VFH83_14140"
     CDS             complement(3508..>5208)
                     /locus_tag="VFH83_14140"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET6487562.1"
                     /translation="FAHLDQRFGGNDLAMVAVESDAVFSTRTLGDIDRLTEAFRAVDG
                     VSSVTSLTNVMDVRKAPGGSVETGKLIDAGNLPATQEGMRALRDYVMGKERYRGSLVS
                     NDGRASLIIVQLAAGSDKNAVVRALQAAAPASAFPETLYFGGIPFLTAELARFILHDF
                     ALLLPIAALLIILTLFFSFGTLRGVLVPLGSVAVTVVWVLGFMSLLRVPLTLVSDIIP
                     AILIAVGTAPCIHILSKYDEDTTLYGQTGPGPSAAFGEVGLRVILAAVTIVLGFCSFI
                     IGSYLTTIRDFGIFTAVGVCFSLLISILLVPAVLTLIPVRPRPAASRRARAPRHPEGR
                     LVYRAMRAWADLVVRRRTAILIASGVVLVAGIVGIPLIQRKADFAEFFDPGSQVRRTE
                     AFITREFGGSRPVQIDFTGDLQNPFILKEMLRFERFLDGEGLAHNAVSPADFVVEMND
                     VMGERRLIPDDPAKVANLLFLVGGQDVARQLLSDDATEGQIQAMVPAQEVKELARTID
                     GIEGYIRGLDTRLVAVDLSVLPPADRQVVAAYRIPRAGRSSCGWPASVSRERRSTPTA
                     "
BASE COUNT          938 a         1717 c         1723 g          830 t
ORIGIN      
        1 tcgatggccc ttcttcacga gaagctgtac cagtccaaga gcatggcggg cgtcgagttc
       61 tccgcgtacg tgcgggacct gacgcgcaac ctcttccagt cctacggcgt caacagcggg
      121 gcctcggagc tggagctgca ggtctccgat gtcacgttgg gcatggacac ggcggtgccc
      181 tgcgggctca tcatcaacga gctggtgtcc aactcgctga agcacgcctt tccgccccgc
      241 caaccgggca aggtgagcat ctccctcaag cccgaccacg cgccgaaggt cgacgcgggc
      301 catccccccg gggagaagtg gtacatgctc acggtggcgg acaagggccg cggtttcccc
      361 gatggcgtcg atttccgcaa caccgactcc ctggggctga agctggtgtg cacgctgacg
      421 gagcagctct tcgggacgat cgacctgaac cgcaacggcg gtaccgagtt cacgattcgc
      481 ttccgagagc tgtaggcggc gccgccggaa tgcggttggc ggccgtaaca gggagttagg
      541 agacattcat ggcgcgttca tggacgcgcc ctcagccgcc gcgtatggtt gaagcaggca
      601 ccggctacgg cgcgcgtgcc gaggaaccat gaatcgactg aacgcggcgg gaccgattct
      661 cgccatcttc tccgctgcgc ttctcaccat gggatgcgca accatcacac cggccaatac
      721 ccagaagccc gatgtccagt tcaccgtgtc ggtggattcc atcccgaggg gagcgtccgt
      781 gtacgcggtg aacgccgacg gcacgctggg agcccttctg ggtctcactc cctaccagtg
      841 gcgcgtcggg ctatcgatgg cgcgcgactc gtccggggcg ccggccttcg gctacgcggc
      901 gcagctcttc ggcccaccgg ggatcagcct ggggaccgtg cacaaggggt acgggttctt
      961 cagcgccgac gagtggtctt ggcagaacct cctgatcagc gttgccgtgg tcaaggaagg
     1021 tctcccaacc acctggtacg cgaatcgggt ggtcgcgagg gtcggtcagg gccccaacgg
     1081 cgcctatccc taccctcccc aggatgtgac cgtcacgttg cccctcggcg cccaggcatc
     1141 cgcactgcca gcccaaccgc cggagacgat catcgtcccg gcgccgtacc cctggccgca
     1201 tcgatgggtg tacccgtatc ggtggcgccg ccaccgaccg taggctcagg ctccctgcgc
     1261 cttctggccg gccgacaggc ggagctggac gatcccgaag agcaggacca gcacgaacag
     1321 ggccgcgaac agaagtccgt gcttgattct gcccaggacg aaccagtaga ccccgtagcc
     1381 gcccagaagg acggacacca cgactgcgac gattccaatg atccgcgaag cgctcttgct
     1441 catggcgcat tgtatcacca accgcggcgg ccggagtagt atggtgtgcg gaggatccat
     1501 gactcgatcc gagcttctgg cgttcttccg gtcgcacatc aacgccgtcg aggcgacggt
     1561 ttccgcggag ggcgcacccc aagccgcggt cgtggggata gccgtcaccg atcagttcga
     1621 gatcgttttc gatagtctgg aaaccacccg caaggccagg aacctgtccc tcagtcccag
     1681 gatcgccctg gtgatcggcg ggtggatcgc gggcgacgaa aggaccctcc agtacgaggg
     1741 cgtcgcggac cggccgagcg gcgcggagct cgaacggctg aaatcggtct actacgaggt
     1801 cttccctacc ggcccaagcc ggctctcctg ggcgggtctg gtctacctcc gggcccgccc
     1861 cacgtgggtc cgatacaccg acttcgggaa gagcccggtc gagaggatcg agctcaacgc
     1921 cgaccaactg cggatgctcg agtagcctac cggctcatgg ccgcctcggt gaacaggcta
     1981 tcggggatgc cggtatcgaa cttcatctcg gagatgatca tccgggtctt gtgctgccgg
     2041 gccaggtcct ccatggtgga ctccatagcc acccagtacc cacccacccg ttggagcttc
     2101 tcccggacca ggaccttcca aagcttaccc gacttgtcgt agtgctcgat gcgcacgggc
     2161 aggaacgtgt ccgtgcgaac ggtcatgacg agcttcgagt agtcgctcac ggcccccggc
     2221 ttgggtgtca gctccagcac gatcgcctcc gggtcctgct tgagaatcct gggatcccag
     2281 cgctccgact cccggcccgc ctccatgtcc tcgtacgtga agtcggtgcc cgtgaagctc
     2341 gccaccttga gctgagcggt gatgcgctgc gcctttccaa aggccggcag atacaggtac
     2401 tgcacgccgt cggggagaga gagaaacgcg atgcccttct ggatggccgg tgccgtgatc
     2461 cgcgcgaggc gccggtccct gcccttctgc cacatctgga gctcctgggt cttctctccc
     2521 ccgaaccggt cgaggagcac cagcttcatc gcgtaggtct ggtccttggc gccgttgacg
     2581 gcgtcatcca cgcggaccag cacatcattc gcggtaaggg cagcctcttg cgccgacaag
     2641 ggccagacca gtgccatggt cagaaccccg atcgcaacgc tcgcggaaag tcctttcatg
     2701 atctcagcat ctccttttgc acccggactg gttttcggcc gccccggaac gctgagggaa
     2761 ccagaagaat gagcgccggc agcagcgtga acgcgcccag cccgctggcc agcatggcga
     2821 tggccgtgag gatcccgaac tgacgcaggg gcagcaggtt ggcaaagagc agcgcaacga
     2881 atcccatggt caccgccagc acgttgatga tgatcgcaag cccggtggtc tggatggtct
     2941 tcttcaccgc ctcgacggcg gtggtctctc cgcggtagaa ggtggcgaac cggaccgaga
     3001 agtggatgac gtagtcgatg ccgatgccca gggcgatgct cccgacgagc accgtggcca
     3061 cgttgatcgg gattccggcg tatcccatca cgccgaacat cgccacgatg gccaggatga
     3121 tgggcacgag gcccagcgcg ccggcccgag cggagcgaag gcgcagcgcc aggagcgcga
     3181 agatgaacac cagggcgatg aggaggctct ctgcctggct ggacaggatg ctgcgatcga
     3241 ggtgccagga gatgagcggc atcccggtga agccgacgga gaaggagatg accggcggct
     3301 ccccctgcgt cgcggtcggg agtcccttga agagcttctc ggggatcgca acggtaggct
     3361 gggagagatc gagcagctcg accgagacat cgccacgaaa cgccggatcc tgctggaggg
     3421 ccgttgggaa cgcctggagc agggccgcgg tggtgccctc cgcggttccc gccgtcccgt
     3481 ccttcatgag gcgcgtgaga agctgggcta cgccgtcggc gttgaccgcc gttcccggct
     3541 gacgcttgcc ggccagccac acgagctcct cccggcgcgc gggatgcggt atgcggcaac
     3601 gacctgccgg tccgctggcg gcaggaccga gaggtctacc gccaccagcc gggtgtccag
     3661 cccgcggatg tacccctcga tcccgtcgat ggtacgggca agctccttga cctcctgggc
     3721 cggcaccatc gcctggatct ggccctcggt ggcatcgtcg ctcagaagct gtcgtgccac
     3781 gtcctgtccc cccaccagga acaggaggtt ggctaccttc gccgggtcat ccgggataag
     3841 gcgccgctct cccatgacgt cgttcatctc cacgacgaag tccgccgggg agacggcgtt
     3901 gtgcgcgagc ccctcgccgt cgaggaaccg ctcgaagcga agcatctcct tcaggatgaa
     3961 gggattctgc aggtcccccg tgaagtcgat ctggaccggg cgcgagccgc cgaactcccg
     4021 ggtgatgaag gcctcggtgc gacggacctg gctgccggga tcgaagaact cggcgaagtc
     4081 cgccttgcgt tggatcaacg gaatgccgac gatgcccgcc accaggacca cgccggacgc
     4141 gatcaggatg gcggtgcgcc ggcgcaccac caggtcagcc cacgccctca tggcccggta
     4201 gacgagccgg ccttccggat gtcggggagc ccgggcgcgc cgtgacgcgg caggccgggg
     4261 ccggaccgga atcagggtca acacggccgg caccagcaga atggagatca ggagggagaa
     4321 gcacaccccg accgcggtga agatcccgaa gtcgcggatg gtcgtgaggt aggagccgat
     4381 gatgaaggag cagaagccca ggacgatcgt taccgccgcc aggatcactc tgagaccgac
     4441 ctctccgaag gccgcggagg ggccgggccc ggtctgccca taaagcgtcg tgtcctcgtc
     4501 gtacttgctg agaatgtgga tgcagggcgc cgtgcccacc gcgatcagga tcgccggaat
     4561 gatgtcggag accagggtca acggcactct gaggaggctc atgaacccca ggacccacac
     4621 caccgtcacc gccacggagc cgagggggac gagcacgccg cggagcgtcc cgaagctgaa
     4681 gaacagcgtg aggatgatga gaagcgcggc gatcggaagg agcagggcga agtcgtgcag
     4741 gatgaaccgg gcaagctcgg ccgtgagaaa ggggatcccg ccgaagtaga gcgtctcggg
     4801 aaaggcggaa gccggggccg ccgcctggag cgcgcggacc accgcgttct tgtccgagcc
     4861 cgccgcgagc tggacgatga tgagggacgc ccttccgtcg ttggagacga ggctgccgcg
     4921 ataccgctct ttgcccatca cgtagtcccg cagggcccgc atgccttcct gggttgcggg
     4981 gaggttcccc gcgtcgatga gcttccccgt ctccacgctc ccgccgggag ccttgcggac
     5041 atccatcacg ttcgtgaggc tggtcaccga ggagaccccg tccaccgccc gaaacgcctc
     5101 ggtgaggcga tcgatgtcgc caagggtcct ggtggagaag acggcgtctg actccactgc
     5161 caccatcgcc aggtcgttgc ccccgaagcg ctggtccagg tgcgcgaa
//
LOCUS       DASRZS010000580         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1103623, whole genome shotgun sequence.
ACCESSION   DASRZS010000580 DASRZS010000000
VERSION     DASRZS010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103623"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1740
                     /locus_tag="VFH83_14145"
     CDS             <1..1740
                     /locus_tag="VFH83_14145"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HET6487563.1"
                     /translation="RLKESLFLRQAEETSRYLMEVPDDSWLYGFRKRAGKPSPGTELF
                     GWYGHGIFHVFGQMLGGMVKLAAGTANPDLATKAIRLAEGWASCMEKDGYCCYKPRGA
                     VNDLHYEYEKLLGGLLDLHEYAGYDKALAWASSMTDWAMANLDREIVHRYREPAKVNF
                     TFYREWYTLSENLYRAFELTGDEKYRTFAKVWEYPDYWNRFLDPVSTLEPCHASSHVN
                     TLSGSARAYRVTGDERYLRIIENAYKRIFPEHTYATGGYGPAEGLFGQPGYLGRMLLE
                     APKNGFGNAEIPCGTWAVFKLTRYLMELTREAPYSAWAERLLYNGIGAELPMHPEGRV
                     MYYANYHVDGASKAVEFEMSALNTTGTALEWPCCSGTYPQAVAEVCNLIGYRTSDGID
                     LCQYVPCTIRWSKTLSFELDTRYPAEEEVTITARVASPADCAVRLRQPDWVGPEAMTV
                     EVNGQPVPSGVDGGWVAVSRRWRDGDRLRLRIPLRLRLDAVDRQKPDLCAVSFGPVTL
                     AANRPGVFTADALHPQLWIEREPGTLSFRSRPGVVKGYPQMSKWFKPFWEYGLGERYH
                     LYNKVERPLSWPT"
     gene            complement(1746..>2194)
                     /locus_tag="VFH83_14150"
     CDS             complement(1746..>2194)
                     /locus_tag="VFH83_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487564.1"
                     /translation="RADQATVFSEAREVKITTKYINSLTLVAVLQDGSTGSYAKVQGY
                     DVGQKYLFTDATFVSTDDLTSADADVNAAILYDVAMGTKDPTIKKNLLKIAATKYPNS
                     IFYPKVQSAQGPAPAAAAPAAAQAPAPAADAGGDAGNGDAGGDSTP"
BASE COUNT          365 a          701 c          754 g          374 t
ORIGIN      
        1 cggctgaagg agagcttgtt cctgcggcag gcggaagaga cgtcgcgcta cctcatggag
       61 gtccccgacg atagctggct gtacggattc cgcaagcggg ccggcaagcc cagccccggc
      121 acggagctct tcggctggta cggtcacggc atcttccacg tcttcggtca gatgctgggc
      181 gggatggtga agctcgccgc gggcacggcc aacccggatc tggcgaccaa ggccatccgg
      241 ctggcggagg gttgggcaag ctgcatggag aaggacggct actgctgcta caagccccgc
      301 ggcgccgtca acgaccttca ctacgagtac gagaagctcc tgggcgggct cctggacctg
      361 cacgaatacg cggggtacga caaggcgctg gcgtgggcat cgtccatgac ggactgggcc
      421 atggccaacc tggaccggga gatcgtccac cgctaccgcg agcctgccaa ggtcaacttc
      481 acgttctacc gggagtggta cacgctcagc gagaacctgt atcgagcgtt cgagctgacg
      541 ggggatgaga agtaccgcac gttcgccaag gtctgggagt acccggacta ctggaaccgg
      601 ttcctggatc ccgtctccac cctggagccg tgtcacgcgt ccagccacgt gaacaccctg
      661 agcggctccg cccgagccta tcgagtcacg ggggacgaac gctacctccg gatcatcgag
      721 aatgcctaca agcggatctt ccccgagcac acctacgcca ctggcggata cggtccggcc
      781 gagggactgt tcggacaacc cggctacctg ggccgcatgc tcctcgaggc tccgaagaac
      841 gggttcggaa acgcggagat cccgtgcggg acctgggcgg tcttcaagct gacccgctat
      901 ctcatggagc tcacgcggga ggcgccttac tccgcgtggg ccgagcgcct gctgtacaac
      961 ggcatcgggg cggagctgcc catgcacccg gagggccggg tcatgtacta cgcgaactac
     1021 cacgtggatg gcgcatcgaa ggcggtggag ttcgagatgt ccgcgctcaa caccaccggc
     1081 acggcgctgg agtggccgtg ctgctccggc acctatccgc aggcggtggc ggaggtgtgc
     1141 aacctgattg gatatcggac ttcggatggg atcgatctct gccagtacgt gccgtgcacc
     1201 atccgctgga gcaagacgct gtcgttcgag ctggacactc ggtaccccgc ggaagaggag
     1261 gtgacgatca cggcccgagt cgcctcaccc gcggactgcg cggtgcggct gcggcagccg
     1321 gactgggttg gcccggaggc catgaccgta gaggtgaacg ggcagccggt gccctcaggc
     1381 gtggatgggg gctgggtagc ggtcagccgc agatggcgcg atggcgaccg tctccggctc
     1441 cgcatcccgt tgcggctgag gctggacgcc gtggaccgcc agaagccgga tctctgtgcc
     1501 gtttcctttg gtcccgtcac cctggccgcc aatcggccgg gcgtcttcac ggcggacgcc
     1561 ctgcaccctc agctctggat cgagcgggag cccggcaccc tctccttccg ctcccgcccc
     1621 ggtgtggtga agggctatcc gcagatgagc aagtggttca agccgttctg ggaatacggc
     1681 ctgggcgagc gctaccacct ctacaacaag gtggagcgcc cgctgagctg gccgacctga
     1741 ggccatcaag gcgtggagtc gcctccggca tcgccgttcc ctgcgtctcc gccggcgtca
     1801 gcggcgggcg cgggggcctg ggccgcggca ggcgctgcgg cggccggcgc cgggccctgc
     1861 gcgctctgga ccttggggta gaagatcgag ttgggatact tggtggccgc gatcttcagc
     1921 aggttcttct tgatcgtcgg gtctttggtg cccatggcca cgtcgtagag aatggccgcg
     1981 ttcacatcgg cgtccgcgct cgtcaggtca tccgtgctca cgaacgtcgc gtcggtgaac
     2041 aggtacttct gcccgacgtc ataaccctgg accttggcat aggagccggt cgaaccgtcc
     2101 tggaggaccg caaccagcgt cagggagttg atgtacttgg tggtgatctt cacctcccgg
     2161 gcttcggaga agacggtggc ctggtccgcc cgga
//
LOCUS       DASRZS010000581         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1104170, whole genome shotgun sequence.
ACCESSION   DASRZS010000581 DASRZS010000000
VERSION     DASRZS010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104170"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VFH83_14155"
     CDS             complement(<1..805)
                     /locus_tag="VFH83_14155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6487565.1"
                     /translation="MMSYRTMGKTGARLSSLGFGCMRLPMKNERTVDRDKAIPLLQRA
                     YSLGINYFDTGKWYCGSDSEKTLGEALKGMDRSKVYVSTKYAFEKPTAADLREKFEAS
                     LRLLDVQYVDFYHLWGISWKSFRTEIDLPGGPLQTFLSLKREGLAKHLSFSFHSDAAD
                     IPKLVDTGIFESMLCQYNLLDRACEEGIAYAASHGLGVAIMGPVGGGRLSAPSPAISR
                     MAPGQNVSTPELALRFVLANPGVTVALSGMSDGKQLEENVATASRDSWLT"
     gene            complement(819..>2093)
                     /locus_tag="VFH83_14160"
     CDS             complement(819..>2093)
                     /locus_tag="VFH83_14160"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HET6487566.1"
                     /translation="CVYPPINRSLIYAAGCESIAITGGGTIDGQGERFPREEESQRPA
                     LIRLRDCRGVRLEGLLLKDYPAWGVHPVHCKGLRVTGLRIDSLVRPNNDGLDIDGCQE
                     VFVSDCTIRSSDDSIALKALQGPEPCRDIVVTNCILTSRCAAIRLGPDAVSPIERVSV
                     SNCVIRDTVLNGIKIQESLGSVMRDIVFSNIVMDNVGGPISIRCAGWKKGASNVWAVF
                     EDTGWEKGEVSGVLFQNIRARVMPGNTPANPKMGISITGTSRTRPRGVAFSGVDISFP
                     GGGTAAEGARRDVPDLEQDYPECYMFGVLPAYGLYAHHADGITLHDIRFRLDSEDLRP
                     AVVCVDVRDVELSGVRAESCREAEAVLRLQNAAGAFVTGSGPLGACGTFLRVEGRRSR
                     DIRVVGNDLGQAPRALELDRDAAPSAVTGPVS"
BASE COUNT          342 a          727 c          680 g          344 t
ORIGIN      
        1 cggtcaacca ggagtcccgg gaggcggtgg ccacgttctc ttccaactgc ttcccgtcgg
       61 acatgccgct caacgccacg gtgaccccgg ggttggccag gacgaaccgg agggcgagct
      121 cgggcgtgct gacgttctgc ccgggcgcca tgcgggaaat cgccgggctt ggcgcgctga
      181 ggcggcctcc gccgacgggc cccatgatgg ccacgccgag gccgtgggac gccgcgtacg
      241 cgatgccctc ctcgcacgcg cggtccagga ggttgtactg gcagagcatg ctctcgaaga
      301 tccccgtgtc cacgagcttc gggatgtcgg cggcatccga gtggaacgag aaggacaagt
      361 gcttcgccag gccctcccgc ttcaggctca agaaggtctg cagcggtccg ccggggagat
      421 cgatctcggt ccgaaagctc ttccaggaga tgccccagag gtggtagaag tcgacgtact
      481 ggacgtccag cagcctgagg ctggcctcga acttctcccg aaggtcggcg gctgtcggct
      541 tctcgaaggc gtacttggtc gagacgtaga ccttgctccg gtccatcccc tttagcgcct
      601 cccccagggt cttctccgag tcggagccgc agtaccactt gcccgtgtcg aagtagttga
      661 tgccgaggga ataggcgcgc tgcagcaggg ggatcgcctt gtcgcgatcc acggtccgct
      721 cgttcttcat gggcaggcgc atgcagccga accccaggga ggacaggcga gcgccggtct
      781 tccccatcgt gcgataactc atcatctccg tgtctccctt acgaaaccgg gccggttacc
      841 gccgacggcg cggcgtctcg gtccaactcc agggcccgag gcgcctggcc cagatcgttc
      901 cccaccaccc ggatgtcccg gctccgccgg ccctccacgc gcaggaacgt cccgcaggcc
      961 cccagaggtc cgctgccggt gacaaaagcg ccggccgcgt tctgcagccg cagaacggcc
     1021 tccgcctccc ggcagctctc cgcccgaacg ccggagagct ccacgtcccg aacgtcgacg
     1081 cagaccacgg ccgggcgcag gtcttcggaa tccagccgga agcgaatgtc gtgcagggtg
     1141 atgccgtccg cgtggtgggc gtacagaccg taggccggca gcacaccaaa catatagcac
     1201 tcggggtagt cctgctccag atccggcacg tcccggcgcg ccccttccgc cgcggtccct
     1261 ccgcccggga agctgatatc cacgccgctg aaggcgaccc cccgcgggcg ggtccgcgag
     1321 gtgcccgtga tcgagatccc catcttcgga ttggcgggag tgtttccagg catgacccgg
     1381 gcgcggatgt tctggaagag gacgccgctc acctccccct tctcccaccc ggtgtcctcg
     1441 aagacggccc agacgttgct ggcccccttc ttccacccgg cgcaccggat cgagatggga
     1501 ccgcccacgt tgtccatgac gatgttggag aacacgatgt cccgcatgac ggagcccaga
     1561 gactcctgga tcttgatccc gtttagcacg gtgtcccgga tgacgcagtt ggagacgctc
     1621 acccgttcga tgggggagac cgcgtccggc ccgagccgga ttgcggcgca gcgggaggtc
     1681 aggatgcagt tggtgaccac gatgtcccga cacggctcag gaccctggag cgccttcagg
     1741 gcgatgctgt catcactgct tcggatggtg cagtccgaga cgaacacctc ctggcagcca
     1801 tcgatgtcca gaccgtcgtt gttggggcgg accaggctgt cgatccgaag cccggtcacc
     1861 cgcaagccct tgcagtggac gggatgcacg ccccaggccg gatagtcctt gaggagcagg
     1921 ccctcgagcc tcacgccgcg gcagtcccga agacggatca gcgccggccg ctggctctct
     1981 tcctccctgg gaaaccgctc tccctggccg tcgatcgtgc cgccgcccgt gatggcgatg
     2041 ctctcacagc cggcggcgta gatcagggaa cggttgatcg gcgggtacac gca
//
LOCUS       DASRZS010000582         4359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3789212, whole genome shotgun sequence.
ACCESSION   DASRZS010000582 DASRZS010000000
VERSION     DASRZS010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3789212"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1781
                     /locus_tag="VFH83_14165"
     CDS             <1..1781
                     /locus_tag="VFH83_14165"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HET6487567.1"
                     /translation="DFLYSAVQDVRVYQVSDVILMTLYVAWLRAYTGWRSADTVFLVL
                     NALAAIVGAGSAFFPAIPQGEFLFGLWVLVTVAYLAVNAALVSPQDAEGAAVVLVSRF
                     PLVAALFILSLIALLYGYEHPVVQAIFVPATYLVPAFLLVTYYTTLHREGAQSVSFFS
                     ANLESTYGFMENLGNAITAKIDLSRVLDLIITAAVKNIGADAGAILMVDEYEDLLRVK
                     ATYGIYPPLAAAPELARVNPASLKRYFAETPIPIGETILGETVKTGKAVLIRDSRLDE
                     RLRANASDDILFVSSLVVVPLVVRNRALGALSVIKRAENQFFEERDFQHLTTLAEFAS
                     ITIDNLYTYIEVLEKREFEREVDIAAQIQQKLLPGELPELESVELSVYNMPAKGVSGD
                     YYDVFRLEKDKLGIAVCDVAGKGIPAALVMVMIRSILHLIVSPERDTAATLNWINRGI
                     TGRIDLDHFATFSFMIYDEKNREILYSNAAHLPLLVYRQATGSLFKVDTQGLPIGIEK
                     GTRYEQKKFHIEKGDLVLLYTDGILEAMNPSGAQYGLDGLKRMVEKNALLPAKELVTA
                     IREDLRRFVGTARQHDDQTLLVMKAL"
     gene            1804..2154
                     /locus_tag="VFH83_14170"
     CDS             1804..2154
                     /locus_tag="VFH83_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6487568.1"
                     /translation="MEIKLRRSGTIYVLDVTGEMDLYNAYKLKEIVTAMIGRQIREYI
                     INLENVQYMDSSGIGALLFVHAELKKRGMILRIANVKGAVKKVIELTKLSGYLPLSAG
                     IQDAMVDIMRTGSR"
     gene            2165..2731
                     /locus_tag="VFH83_14175"
     CDS             2165..2731
                     /locus_tag="VFH83_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6487569.1"
                     /translation="MEQAKQILVDGKSPLFSTAGMFHKTFASDFRQIRYFTLMVVQKA
                     PTEIREINLLEQQISELIKNAVKHGNKSDPSKSIHVWFQFTEDSARLIVQDSGAGFTR
                     LEEWNEFNRKRTECFQKQDFEAMANYVSFITKDSDEHDSGNALFAALEYWDGGVVFNE
                     ARNCVAVARSFPRRRHWIDLQENEGAGE"
     gene            2793..3959
                     /locus_tag="VFH83_14180"
     CDS             2793..3959
                     /locus_tag="VFH83_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487570.1"
                     /translation="MTRKERNPIIDLPVKVLFTTDGAEWFIRNHQKPHRVRMADRRQG
                     YGISLNNFSAASLQKMFTIDYVSAVEIARTDFSEKGGEIVDLTKLITYRVLYRAFELE
                     TYRMFLASPLIQNWNRAHPSKTIDERSAIHNGQLRALVQSCSPELPLVSEDIQKAVLR
                     DIEEDPKLGPEEKLVLYHLCERYVDSMRDLMRCVLSRSRGQREYHRLVFDICALLRNY
                     LERSKIAEYLALIIMELIAYTETVHSLEIARRLFPRTQPTTEMLRNPSLRNAISRDMN
                     EQRDYLYLTYHVSNEASSLGTENRLKVVLFNGVREYQKMKSQIETKLGLDLEEKGLQD
                     LYRRLPKQDTDSELGLYYLSFLESECSKCGVHLDSHVNESLTSDLTVITLNLQF"
     gene            4013..>4359
                     /locus_tag="VFH83_14185"
     CDS             4013..>4359
                     /locus_tag="VFH83_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET6487571.1"
                     /translation="MRTRLRASVLLALALIASGSAAQTKGLPDLKGRGIRVVTEGRYP
                     PLSFLDPSSGKPAGLDCDLLTEIARRLNARVTWSKTTWTDLLKSVHEGRYDIAANGIT
                     ITADRKEQVDFSDP"
BASE COUNT          832 a         1447 c         1361 g          719 t
ORIGIN      
        1 gtgacttcct gtacagcgcc gtgcaggacg tccgcgtcta ccaggtgtcg gacgtcatcc
       61 tcatgacgct gtacgtggcc tggcttcgcg cttacaccgg atggcggagc gccgacacgg
      121 tcttcctcgt cctgaacgcc ctggcggcga tcgtgggggc gggctctgcc ttcttccctg
      181 ccattcccca gggagagttc ctctttggcc tgtgggtcct cgtcaccgtg gcgtacctcg
      241 cggtgaacgc ggccctcgtg tccccgcagg acgcggaagg cgctgcggtg gtgctcgtaa
      301 gccgctttcc cctggttgcg gcgctcttta tcctctctct gatcgccctg ctgtacggct
      361 acgagcaccc ggtggtccag gcgatcttcg tgccggccac gtatctggtt cccgcgttcc
      421 tgctggtcac gtactacacg actctccatc gcgagggggc gcagtcggtc agcttcttct
      481 ccgccaacct ggagtccacc tatggcttca tggagaacct gggcaacgcc atcaccgcca
      541 agatcgacct ctcccgggtc ctggacctga tcatcacggc cgcggtgaag aacatcgggg
      601 cggacgccgg cgccatcctg atggtggacg agtacgagga cctccttcgg gtcaaagcca
      661 cctatgggat ctacccgccg ctggcggcgg cgcccgagct ggcccgggtc aaccccgcca
      721 gcctcaagcg gtacttcgcg gaaaccccga tccccatcgg ggagaccatc ctgggggaga
      781 ccgtgaagac gggcaaggcg gtcctcatcc gcgactcccg cctggacgag cgtctgcgcg
      841 ccaacgcctc ggacgacatc ctcttcgtga gctcgctggt ggtcgtcccc ctggtggtcc
      901 gtaaccgggc cctgggcgcg ttgtccgtca tcaagcgcgc cgagaaccag ttcttcgagg
      961 agcgggactt ccagcacctc accaccctgg ccgagttcgc ctccatcacg atcgacaacc
     1021 tctataccta catcgaggtg ctggagaagc gggagttcga gcgggaggtg gacatcgccg
     1081 cccagatcca gcagaagctc ctgcccggcg agctccccga gctggagagc gtggagctct
     1141 ccgtctacaa catgcccgcc aagggcgtga gcggggatta ctacgacgtg ttccgcctgg
     1201 agaaggacaa gctgggcatc gcggtctgcg acgtggcggg caaggggatc ccggcggcgc
     1261 tcgtgatggt catgatccgg tccatcctcc acctgatcgt gtccccggag cgggacacgg
     1321 cggccaccct gaactggatc aaccggggca tcacgggccg catcgacctg gaccatttcg
     1381 ccaccttctc cttcatgatc tacgacgaga agaaccggga gatcctctac tccaacgcgg
     1441 cccacctgcc gctcctggtc tatcgccagg ccaccggctc cctgttcaag gtggacacgc
     1501 aggggctccc catcgggatc gagaagggga cccgctacga gcagaagaag ttccacatcg
     1561 agaagggtga cctcgtcctc ctgtatacgg acgggatcct cgaggccatg aatccctcgg
     1621 gggcgcagta cgggttggac ggcttgaagc ggatggtgga gaagaacgcc ctgctccccg
     1681 caaaggagct ggtgacggcc atccgcgaag atcttcgccg cttcgtcggg accgcccgac
     1741 agcacgacga ccagacgctg ctggtgatga aggccctgtg acgcgcgcaa ccgagggatg
     1801 gccatggaga tcaagctgag gaggtccggc acgatctacg tcctggacgt gacgggagag
     1861 atggacctct acaacgccta caagctcaag gagatcgtca ccgcgatgat cggacgacag
     1921 atccgggagt acatcatcaa cctggagaac gtgcagtaca tggattccag cggcatcggc
     1981 gcgctgctct tcgtccacgc cgagctcaag aagcgcggga tgatcctgcg gatcgccaac
     2041 gtcaagggcg ccgtgaagaa ggtcatcgag ctcaccaagc tctcggggta cctgcccctc
     2101 tccgcgggga tccaggacgc catggtggac atcatgcgga ccgggtcccg ctaggaggag
     2161 aggcatggaa caggccaagc agatcctcgt ggacgggaag agcccgctgt tctcaacggc
     2221 ggggatgttc cacaagacgt tcgcgagcga tttccgccag atccgttact tcaccctgat
     2281 ggtggtccag aaggctccga cggagatccg cgagatcaac ctcctggagc agcagatcag
     2341 cgagctcatc aagaacgccg tgaagcacgg gaacaagagc gacccgtcga agagcatcca
     2401 cgtctggttc cagttcacgg aggacagcgc gcggctgatc gtccaggact ccggcgccgg
     2461 gttcacgcgc ctggaagagt ggaacgagtt caaccgcaag cgcaccgagt gcttccagaa
     2521 gcaggacttc gaggccatgg ccaactacgt ctcgttcatc accaaggaca gcgacgagca
     2581 cgacagcggc aacgctctgt tcgccgccct ggaatactgg gacggcgggg tggtgttcaa
     2641 cgaggcccgc aactgcgtcg ccgtggcccg cagcttcccg aggcgccgtc actggatcga
     2701 cttgcaggaa aacgaagggg cgggggaatg agccgggccg cgccgcgcgg gggccgtaga
     2761 ggccagccgc aagcgtggag gagcagatgc ctatgacccg aaaagagcga aacccgatca
     2821 tcgacctccc cgtcaaggtc ctcttcacca ccgacggcgc ggagtggttc atccgaaacc
     2881 atcagaagcc ccaccgcgtc cgcatggcgg accggcgtca gggctacggg atctccctga
     2941 acaacttctc ggcggcttcc ctgcagaaga tgttcaccat cgactacgtc tccgccgtgg
     3001 agatcgcccg gaccgatttc tccgagaagg gcggggagat cgtggacctg accaagctca
     3061 tcacctaccg ggtcctttac cgcgccttcg agctggagac ctaccggatg ttcctggctt
     3121 cccccctgat ccagaactgg aaccgggccc atccctccaa gaccatcgac gagcgctccg
     3181 ccatccacaa cggacagctc cgcgccctgg tgcagagctg ctctcccgag ctgccgctgg
     3241 tttccgagga catccagaag gcggtcctgc gggacatcga ggaggacccc aagctgggcc
     3301 cggaagagaa gctggtcctg taccacctct gcgagcgcta cgtcgacagc atgcgggacc
     3361 tcatgcgctg cgtcctctcc cgctcccgcg gccagaggga ataccatcgc ctggtgttcg
     3421 acatctgcgc gctcctgcgc aactacctgg agcgctccaa gatcgcggag tacctggcgc
     3481 tgatcatcat ggagctcatc gcctacacgg agaccgtcca ctccctggag atcgcccgca
     3541 ggctcttccc gcggacccag cccaccacgg agatgctgag gaaccccagc ctgcgcaacg
     3601 cgatctcccg ggacatgaac gagcagcggg actacctgta cctcacctac cacgtcagca
     3661 acgaggccag ctccctggga acggagaacc gcctcaaggt ggtcctgttc aacggcgtgc
     3721 gggagtacca gaagatgaag agccagatcg agaccaagct cggtctggac ctggaggaga
     3781 agggcctgca ggacctgtac cgccggcttc ccaagcagga cacggactcc gagctggggt
     3841 tgtactacct ctcattcctg gagtccgagt gctccaaatg cggcgtgcac ctggactccc
     3901 acgtgaacga gagcctcacg agcgacctga cggtgatcac cctgaacctg cagttctgag
     3961 gtcgtagcgc aacggttgtc ttgcggatac gatgcggtcc gggaggggat ggatgcgaac
     4021 gcgcctccgc gcatcggtgc tcctggccct ggcgttgatc gcctcggggt cggcagcgca
     4081 gaccaagggc ctgccggacc tgaagggccg cggcatccgc gtggtgaccg agggccgcta
     4141 cccgcccctg agcttcctgg atccgagctc cggcaagccc gcggggctgg actgcgacct
     4201 tctcacggag atcgcccggc gcctcaacgc gcgggtcacc tggagcaaga cgacgtggac
     4261 cgaccttctc aaaagcgtgc acgaggggcg ctacgacatc gccgccaacg ggatcacgat
     4321 caccgcggac cgcaaggagc aggtggactt ctccgatcc
//
LOCUS       DASRZS010000583         5456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2232976, whole genome shotgun sequence.
ACCESSION   DASRZS010000583 DASRZS010000000
VERSION     DASRZS010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2232976"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1795)
                     /locus_tag="VFH83_14190"
     CDS             complement(161..1795)
                     /locus_tag="VFH83_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6487572.1"
                     /translation="MKGFTFRRALIALGACLVLLGSQALFAEKVIYLPSDIEFDNLDP
                     RVLTSTGHQLVQFAMFDPLVRTHDGKLLPGAASSWEMSKDGMTYTFHLRDAKWSDGKT
                     VTGGDFVHAFVRMFQICPASPIYDDIKNGAELRAGKVPPEQLGVKAPDDKTVVITLKN
                     PAPYFMGLIASSFGAPGRADLVAKYGEAYGASAESLASNGPFILTEWKKEDHVTLVKN
                     PNWWDAKDIKIDKIIVYVLPDASTQRNLFDSGQLDYYRPRSEAESKAYESQGLLVRYV
                     AGGIRDLQINRHGENDPVKARILSNPNFMKAISYAIDRQGYIDKVLQGAGYPATVQTP
                     PATGVYPGKTWGEVSTNIGKFHPVTADPAKSKMYMDLVLKDMGYTSVDQLPTFDFLTS
                     VSPDDPKDVTGYILGVFRSMGLKINVKLATGQQFYNNLYKPALAYDICRAGWGPDFDD
                     PATYMGYWTTQSTDMGVTYENAQFDKLYFAANMETDLVKRAAILNQAEALFSDNAPCI
                     PLMWNKGSAALSARVKGFTFALTGLTTDYIFADLTK"
     gene            complement(1974..3260)
                     /locus_tag="VFH83_14195"
     CDS             complement(1974..3260)
                     /locus_tag="VFH83_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET6487573.1"
                     /translation="MSETVNVRQRRDRGFPQILGARAQSDLITEMVRDRLDTILPRAM
                     QAAGVDMWIVLSQEDDLDPVYKTMIPMDCWCPILQMLVFVDEGAGKYSKYSISGTDTK
                     DLYARPYAGQLEEKQWPELLALVERRDPQTIGVNIGSVAWAGGGLSHNLYRQLQERLP
                     KKYADRLVSAEAAAVIWGSCLTDREVDIFRHVVEIGHCIIADSFSRMTITPGVTTVDD
                     LVWYYWQRSIDLGLELAFRPYFRLMRPEATARIYGPNDKVIRPGDGIHCDVGIKYLRL
                     NSDNQHLAYVLRRGESDAPQGLKALMTQTNRLQDIYLSEFKAGLSGNVLLQNILSRAR
                     REGINTPRVYSHNLGLFLHEPGPLIGLPWEQEKPLPRGEVKLQANQAFTMELSTDGPV
                     PEWGGQVVRCAMEEPVVFTAQGCATLRGRQTEYYLI"
     gene            complement(3288..4955)
                     /locus_tag="VFH83_14200"
     CDS             complement(3288..4955)
                     /locus_tag="VFH83_14200"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET6487574.1"
                     /translation="MSSDEPWVEKTLAGMSLARRVAQMVTVGIEGGYLSDDDPRFQHG
                     ARLARELGVGGFVVYGGTPRDIARLLNRLQAEAAVPLLMSSDFEGGPGQQAAGASEFP
                     GDMALSAVGAEELAYRVATIGATEGRAMGIHLTYSPVADVSVDPGNPAESVRSFGGDL
                     DLLGRLIAAYIRGYQENGMLATAKHFPGRGDVQRLANWPPFTYIAKPAAQVEAQEFRA
                     FSHAIAAGVACVMTEHVAVPSVTGGSELPASVEPALATGWLREKLGFSGILSSDDLWY
                     DFVVQRFGAEEVAVKAIQAGHDVLLKPRDPDAAVERVVRAVESGEIPRERIDESVRKL
                     LSAKARLSLHRERFTDESRLGALVGTTAHLAVVQELADRSLTLLRNDGVLPVASASLK
                     QIVNLSFQKAESDPSPATLAGKLSAAFPGTQSLTIRPEMESEAYERALRLARSASLVV
                     LSLFVPRDRIGDPAPLRGADQALIRRILSAKPGAVIAMSYGNPFLVERLEGVPAFAVG
                     YGEKGWFGNQAVYFESFVKLLRGELRPQGRLPVKVSSTFPIGSGIRG"
     gene            complement(4952..>5456)
                     /locus_tag="VFH83_14205"
     CDS             complement(4952..>5456)
                     /locus_tag="VFH83_14205"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET6487575.1"
                     /translation="FGAQSAEEVVAAGMGEIPQRSRMWELLSSVVRQWRDGVEWQTVL
                     AGVTERCKVYKRPHVLPNVARLAIGLLYGDMDVRKTLCITVMCGSDPDCTAGAAGSIV
                     GVRAGVGGIPEDFREAAGDRLPTAVLGFHDTRISLVAEAAEQILQGSPFRGEARATEA
                     AGEARRR"
BASE COUNT          924 a         1828 c         1731 g          973 t
ORIGIN      
        1 caccaggacg cggttcagga tgtacctcac catggtcccc cctttcccag gccgttgggg
       61 cggcctctgg agccctcgtt ctggcgggtg gtgagggatc cgcgcaccag agacaatgga
      121 ggcgccccgg gccggggcgc ctcccgagat acgatccgct ctacttggtc aggtccgcga
      181 agatgtagtc cgtggtcagc ccggtgaggg cgaaggtgaa gcccttcacc cgagcgctca
      241 acgccgcgct gcccttgttc cacatgaggg ggatgcaggg tgcgttgtcg gagaacagcg
      301 cctcggcctg attcaggatg gcggcccgct tgaccaggtc cgtctccatg ttcgccgcga
      361 agtatagctt gtcgaactgg gcgttctcgt aggtcacgcc catgtcggtc gactgcgtgg
      421 tccagtagcc catgtaggtg gccgggtcat cgaagtcagg accccagccg gcgcggcaga
      481 tgtcgtacgc cagggcaggc ttgtacaggt tgttgtagaa ctgctgtccc gtggcgagct
      541 tcacgttgat cttcagaccc atggaccgga acactcccag gatgtagccg gtgacgtcct
      601 tgggatcatc cggagaaacc gaggtcagga agtcgaacgt ggggagctgg tccaccgagg
      661 tataccccat gtccttgagg accaggtcca tgtacatctt cgacttggca ggatccgcgg
      721 tcacggggtg gaacttgccg atgttggtgc tgacctctcc ccaggtcttc cccgggtata
      781 ccccggttgc gggaggagtc tggaccgtgg ccggataacc ggctccctgg agcaccttgt
      841 cgatgtaccc ctggcggtcg atggcgtacg agatggcctt catgaagttc gggttgctga
      901 ggatccgcgc cttcacgggg tcattctcgc cgtggcggtt gatctggaga tcccggatac
      961 ccccggccac gtaacgaacc agcaacccct gggactcgta ggccttggac tcggcctcgc
     1021 tgcggggccg gtagtagtcg agctggccgc tgtcgaacag gttgcgctgc gtgctggcgt
     1081 cgggcaggac gtagacgatg atcttgtcga tcttgatgtc cttggcatcc caccagttgg
     1141 ggttcttcac cagcgtgaca tggtcttcct tcttccactc ggtgaggatg aagggtccgt
     1201 tgctggccaa agactccgcg ctggcgccgt acgcctctcc gtacttggcc accaggtccg
     1261 cgcgccccgg agcgccgaag gagctggcga tcagacccat gaagtacggg gctgggttct
     1321 tcagcgtgat caccaccgtc ttgtcatccg gcgccttcac gcccagttgc tccggcggca
     1381 ccttcccggc gcgaagctcg gcgccgttct tgatatcgtc gtagatcggg ctggccggac
     1441 agatctggaa catgcgcacg aaggcgtgca cgaagtcccc gccggtcacc gtctttccgt
     1501 cggaccactt ggcgtcccgc agatggaacg tgtaggtcat tccatccttg gacatctccc
     1561 agctgctggc cgcaccgggt aggagcttgc cgtcgtgcgt tcgaacgagc gggtcgaaca
     1621 tcgcgaactg gacaagctgg tgccccgtgg acgtcaagac tcgaggatcg aggttgtcga
     1681 actcgatgtc cgacggaagg tagatgacct tctccgcgaa cagggcctgc gagcccagca
     1741 agacgagaca cgcgcccagt gcaatcagcg cgcgtctgaa tgtgaagccc ttcattctct
     1801 gcctcctctc ttttgtctcc ccaccaccgg tgggtaatct ctgctccgaa accctccttg
     1861 ggtcacggtt gaattgtata attgtctgac aatagacaac catgtccaat gtccgctgtc
     1921 aagttatgcc ctccgcggcc tctcggcgcg gtctgccgag ggcgagcgtc cgctcagatg
     1981 aggtaatact cggtctgtcg accccgcagg gtggcgcacc cctgagccgt gaacacgacc
     2041 ggctcctcca tggcacaccg cacgacctgc ccgccccact ccggcaccgg gccgtcggtg
     2101 ctcagctcca tcgtgaaggc ctggttggcc tgcagcttca cctcgccgcg aggcagaggc
     2161 ttctcctgct cccacggcag cccgatgagc ggacccggct cgtgaaggaa gagtcccagg
     2221 ttgtgcgagt agacccgcgg ggtgttgatc ccctctcgac gggcccggct caggatgttc
     2281 tgaagcagca cgttgccgga gagtccggcc ttgaactcgc tcaggtagat gtcctggagc
     2341 cgattggtct gggtcatgag ggccttgaga ccctgcgggg catcgctctc gccccgacgg
     2401 agcacgtagg ccagatgctg gttgtccgag ttcagccgca ggtacttgat ccccacgtcg
     2461 cagtggatgc cgtccccagg gcggatcacc ttgtcgttgg gaccgtagat tcgcgcggtg
     2521 gcctcaggac gcatcagccg gaagtagggc cgaaaggcga gctccagtcc caggtctatg
     2581 gacctctgcc agtagtacca gaccaggtcg tcgaccgtgg tcactcccgg ggtgatcgtc
     2641 atgcggctga agctgtcggc aatgatgcag tgcccgatct ccacgacgtg gcggaagatg
     2701 tcgacttcgc ggtccgtgag gcaggaaccc cagatcacgg cagccgcctc cgcggaaacc
     2761 agccgatcgg catacttctt cggaagcctc tcctggagct gtcggtacag gttatggctc
     2821 agcccgccgc cggcccatgc cacggagccg atgttcacgc cgatcgtctg cggatcccgc
     2881 ctctccacca gggccaggag ctcgggccac tgcttctcct cgagctgccc cgcgtacggc
     2941 cgcgcataga gatctttggt atcggtcccg gagatgctgt acttgctgta cttgccggct
     3001 ccctcatcca cgaacacgag catctgcagg atggggcacc agcagtccat cggaatcatg
     3061 gttttgtaga ccggatccag gtcgtcctcc tgggaaagga cgatccacat atccacgccc
     3121 gcggcctgca tggcgcgggg caggatggta tcgagccggt cccggaccat ctcggtgatc
     3181 aggtcggact gcgcccgcgc gccaaggatc tgggggaacc cccggtcccg cctctgtcgg
     3241 acgttcacgg tttcagacac gaaatcctcc tttcaagact actggggtca cccgcgaatc
     3301 ccggacccaa tgggaaacgt cgagctgacc ttcaccggta gcctgccctg gggtctcagc
     3361 tctccgcgaa ggagcttgac gaacgactcg aagtagacag cctggtttcc gaaccagccc
     3421 ttctccccgt acccgaccgc gaacgctggc acgccctcca gacgctccac caggaacggg
     3481 ttgccgtacg acatcgcgat cacggccccc ggtttcgccg agagaatgcg acggatgagg
     3541 gcctggtccg cgccgcgcag aggggccgga tccccaatgc gatcccgggg tacaaacagg
     3601 gaaaggacga cgagggaggc ggagcgtgcc agccgcagcg cccgctcgta ggcctcgctc
     3661 tccatctcgg gacggatcgt gaggctttga gtgccgggga acgcagcgga tagctttccc
     3721 gcgagagtcg ccggggatgg gtcactttcc gccttctgga agctcaggtt cacgatctgc
     3781 ttgagggagg ccgaggccac cggaagaacg ccgtcgtttc gaagcagggt cagggaccgg
     3841 tccgccagct cctgcaccac ggccaggtgg gcggtggtgc ccaccagggc gcccaggcgg
     3901 gactcgtcgg tgaagcgttc ccggtgcagg ctcaggcgag ccttggcgga cagaagcttg
     3961 cggaccgatt cgtcgatgcg ctcccgcggg atctcgccgg actccacggc ccgaaccacc
     4021 cgctccaccg ccgcatccgg atcccgaggc ttcaggagca cgtcgtgacc cgcctggatc
     4081 gcctttaccg ccacttcctc cgcgccgaag cgctggacga cgaagtcgta ccacaggtcg
     4141 tccgacgaca ggatgccgga gaacccgagc ttctcgcgga gccacccggt ggccagggcc
     4201 ggctcgacgc ttgcgggaag ctcggacccg ccggtcacgc tggggaccgc cacgtgctcg
     4261 gtcatcacgc aggccacccc ggccgcaatg gcatgggaga acgcgcggaa ctcctgggcc
     4321 tccacctggg cagcgggctt ggcgatgtac gtgaaggggg gccagttggc cagccgttgc
     4381 acgtccccgc ggcccgggaa atgcttggcc gttgccagca tcccgttctc ctggtaaccg
     4441 cggatgtaag cggcgatcag gcgtcccagg agatccaggt ctcctccaaa cgagcgaacg
     4501 ctctccgcgg ggttgcccgg atccaccgac acgtcggcaa ccggggaata ggtcaggtgg
     4561 atgcccatgg cccggccttc cgtggccccg atcgttgcca ctcgataggc cagctcctcg
     4621 gctcccactg cggacagcgc catgtccccc ggaaactcgc tggcgccggc ggcctgctgc
     4681 cccggaccgc cttcgaagtc cgaggacatc aggagcggca ccgcggcctc cgcctggagc
     4741 cggttcagaa gccgtgcgat gtctcgagga gtgccgccat agacgacgaa cccgcccacg
     4801 cccagctccc gggccagacg agccccgtgc tggaaacgag gatcgtcatc ggacaggtag
     4861 ccgccctcga tcccgacggt caccatctgc gcaacccgtc gggcaaggga catcccggcc
     4921 agagtctttt ccacccacgg ctcgtcgctg ctcatcgccg cctcgcctcc cctgcagcct
     4981 ccgtcgctcg ggcctcgccc cgaaaggggc tcccctggag gatctgctct gccgcttccg
     5041 ccacgagact gatccgggtg tcgtggaacc cgagcaccgc cgtgggcagc cgatccccgg
     5101 ccgcctcgcg gaagtcctcc gggattcccc cgacaccggc gcgcacgccg acgatggagc
     5161 cggcggcgcc cgccgtgcag tccggatcac ttccgcacat gaccgtgatg caaagggtct
     5221 ttcgaacgtc catgtcgccg tacaggaggc cgatcgccag ccgcgccaca ttgggaagaa
     5281 cgtgcggacg tttgtagacc ttgcagcgct ccgtgacacc ggcaagcacg gtctgccact
     5341 ccacaccgtc ccgccattgt cggaccacgg aggagaggag ctcccacatc cgcgagcgct
     5401 gggggatctc gcccatgccg gcggcaacca cctcttcggc gctctgggca ccgaac
//
LOCUS       DASRZS010000584         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1866113, whole genome shotgun sequence.
ACCESSION   DASRZS010000584 DASRZS010000000
VERSION     DASRZS010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866113"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1615)
                     /gene="ppk1"
                     /locus_tag="VFH83_14210"
     CDS             complement(<1..1615)
                     /gene="ppk1"
                     /locus_tag="VFH83_14210"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HET6487576.1"
                     /translation="MAEIRSLELTDPAIYIDRELSLLDFFRRVLEEAEDPANPLLERV
                     RFLGIASSDLAEFFMVRVSGLKQQIEAGVTDQTPAGLTPSEQLQAVRKASQELMERTR
                     ESLREVLPPLEQAGIRIRSYDALEPTQKAQVDHFFDQVVFPVLTPLALDPGRPFPHIS
                     NLSLNLALVITDSAGEERFARVKLPATLPRLVEIKASGPRDGEHTFVWLDQVVAANLG
                     SLFPGMRITQAYPFRVTRDAEVSIQELEAEDLLETIEQGVRRRRFGKVVRVTVDPEMP
                     AGVRQILVEHLVVDPADIYTLEPPLGMSDIGQLYSVPRPDLKYPPLVPTVPPQLQEPG
                     GSIFAAIRREDILLHHPYDSFGPVVEFLGAAARDPAVLAIKQTLYRVGRNSPVVESLL
                     KAARNGKQVAVLVELKARFDEESNIEWARALEREGVHVVYGLLGLKTHSKIALVVRQE
                     GDRIRRYVHLSTGNYNVVTAQQYTDIGLFTCDDEIGADASDVFNYLTGYSEKREYRRF
                     LVAPISLRSGLERLIRREIEHQREGGTGHL"
     gene            complement(1608..>2242)
                     /locus_tag="VFH83_14215"
     CDS             complement(1608..>2242)
                     /locus_tag="VFH83_14215"
                     /inference="COORDINATES: protein motif:HMM:NF044477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6487577.1"
                     /translation="LAEKGVSRRAKEEEGVLEGAVALGRKYSFDEGHALHVARLAGQL
                     FDQLVSLHKLGSRDRRILIAAAILHDIGEYVSLKGHHKHTLYLISRSELTGFTAAEQF
                     LVANVARYHRKGAPSLQHPEFALLSAADRSRVRRMASLLRIADALDKEHREKLSRIAV
                     RQRDGRVEILAGRGGELLLEKWALEKKADLFRRTFGAPVFLIPQEPDSDG"
BASE COUNT          359 a          745 c          765 g          373 t
ORIGIN      
        1 tcaggtgtcc cgtcccgcct tcccgctggt gctcgatctc tcgccggatg agtctctcca
       61 ggccggagcg caggctgatg ggagccacca ggaaccttcg gtactcgcgt ttctccgaat
      121 accccgtgag gtagttgaag acatcggagg cgtcggcccc gatctcgtcg tcacaggtga
      181 agagcccgat gtccgtgtac tgctgcgcgg taacgacgtt gtagttcccc gtggaaagat
      241 ggacgtagcg gcggatccga tcgccctcct ggcggaccac cagcgcgatc ttcgaatggg
      301 tcttgaggcc cagaaggccg tagaccacgt ggacgccttc ccgctccagc gcgcgggccc
      361 actcgatgtt gctctcctcg tcgaaccgcg ccttcagctc caccagcacg gcgacctgtt
      421 tgccgtttcg cgcggccttg aggagggact ctacgaccgg ggagttcctg ccgacgcggt
      481 agagggtctg cttgatggcc agcacggcgg gatcccgcgc cgcggcgccc aggaactcca
      541 ccacggggcc gaaggagtcg taggggtgat ggaggaggat atcttcccgc cggatcgcgg
      601 cgaagatgct cccgccgggc tcctggagct gcggcggtac ggtcgggacc agcggggggt
      661 acttcaggtc cgggcgcggc acgctataca gctgcccgat gtcgctcatt cccagcgggg
      721 gctccagcgt atagatgtcg gcgggatcca ccaccaggtg ctccaccagg atctggcgca
      781 cgcccgccgg catctccgga tccaccgtga cgcgtacgac cttgccgaac cttctccgcc
      841 tcacgccctg ttcgatggtc tccaggaggt cctcggcctc gagctcctgg atggagacct
      901 ccgcatcccg cgtgacgcga aacggatacg cctgggtgat gcgcatgccg gggaacaggg
      961 agcccaggtt ggccgctacc acctggtcca accacacgaa ggtgtgctcg ccgtcccggg
     1021 ggccggaggc cttgatctcg acgagccggg gcagcgtcgc cgggagcttt acccgggcaa
     1081 accgctcctc cccggcggag tcggtgatga ccagcgcgag gttcagactg aggttggaaa
     1141 tgtgggggaa cggccggccc ggatccaagg ccagcggggt gaggacggga aagaccacct
     1201 ggtcgaagaa gtgatccacc tgcgccttct gggtgggctc cagcgcgtcg tacgagcgga
     1261 tccggatgcc cgcctgctcc agcgggggca ggacctcccg aaggctctcg cgggtccgct
     1321 ccatgagctc ctgggaagcc ttgcgcacgg cctggagctg ctcggagggc gtgaggccgg
     1381 ccggggtctg gtccgtcacg ccggcctcga tctgctgttt caggcccgac acccggacca
     1441 tgaagaactc ggccagatcg gagctggcga tccccaggaa ccgcacccgc tccagcagcg
     1501 ggttggccgg gtcttcggct tcctccagca cccggcggaa gaagtccagg aggctcagct
     1561 cccggtcgat gtagatcgcg gggtcggtga gctccaggga tcggatctca gccatcggaa
     1621 tccggctctt gcgggatcag gaagacgggg gcgccgaagg tccttcggaa caggtcggcc
     1681 ttcttctcca gggcccactt ttccaggagc agctccccgc cacgaccggc caggatctcc
     1741 acccggccgt cccgttggcg gaccgcgatc cgcgagagct tctcccgatg ctccttgtcc
     1801 agcgcgtcgg cgatgcgaag cagggaggcc atgcggcgga cccgggaccg gtccgccgcg
     1861 gagaggagcg cgaactcggg atgctggagg ctgggggcgc ccttgcgatg gtagcgggcc
     1921 acgttggcca ccaggaactg ctccgccgcg gtgaacccgg tgagctccga acgggagatg
     1981 agatacaggg tgtgcttgtg atggcccttc aggctcacat attcgccgat gtcatggagg
     2041 atcgccgcgg cgatcaggat ccgccggtcc cgactcccca gcttgtgcag ggagacgagc
     2101 tggtcgaaga gctgaccggc cagccgtgct acgtgcagag cgtgaccttc atcgaaggag
     2161 tacttgcggc cgagggccac ggctccttcc aggaccccct cctcctcctt ggcccgccgt
     2221 gagacgccct tctccgccag gc
//
LOCUS       DASRZS010000585         2002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_324593, whole genome shotgun sequence.
ACCESSION   DASRZS010000585 DASRZS010000000
VERSION     DASRZS010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324593"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VFH83_14220"
     CDS             <1..737
                     /locus_tag="VFH83_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6487578.1"
                     /translation="MGERELRAQRRNLQIIFQDPFASLNPRMTIGDILAEAFIIHGER
                     RKKELATRVERLLEQVGLQPDHARRYPHQFSGGQRQRIGIARAIGLNPKLIVCDEPVS
                     ALDVSIQAQIINLLEDLQDRLGLTYLFIAHNLSVVKHIADRVAVMYLGKIVEISSTKN
                     VFDHTLHPYTEALLSAVPVPDTRAERKRVILRGEVPSPRNPPPGCHFHTRCPYAMPIC
                     RTVEPRLEDRGGDHLVSCHLPSLQPA"
     gene            complement(836..>2002)
                     /locus_tag="VFH83_14225"
     CDS             complement(836..>2002)
                     /locus_tag="VFH83_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487579.1"
                     /translation="REKAGLWRKLNDLESSRPMVWINEIPWHEMNVEDELTLRTVHPW
                     ARELETRLRRTLYQWRHMPGDMVFSDFLECPLAVHSTDFGIHEDVDVVRTDAANEIVS
                     RHFHVQIRQPEDIEKIRLPRVTHDQETTQVVFQAMQGLFRDIIPVRKVGQSHIWFTPW
                     DYLIRWWGLQEAMIDLVERPALVNAAVERMVDAWMIELDQFEAQNLLALDCSNTRIGS
                     GGYGYTWDLPGADFDPGRVRPANMWGCSNAQIFSEVSPEMHWEFALRHDLRWLKRWGL
                     TYYGCCEPLDRKIGLLRKIPNLRKISMSPWNDAERILREVGGDYVLSFKPSPAIFVEE
                     TWNPQKARAAMTSVLEQARGAPIEVIMKDISTVCYKPQRLWDWARIAMEVVQEA"
BASE COUNT          338 a          698 c          618 g          348 t
ORIGIN      
        1 ggatgggaga gcgggagctg cgcgcgcagc gccgcaacct ccagatcata ttccaggacc
       61 cctttgcgtc gctcaatccc cgcatgacca tcggcgacat cctggcggag gcattcatca
      121 tccacgggga acgacggaag aaggagcttg ccacccgggt ggagcgcctg ctcgaacagg
      181 tcggcctgca gcccgatcac gccagacgct atccccacca gttcagcggc ggccagcgcc
      241 agcgcatcgg aatagcgcgc gccatcggcc tgaaccccaa gctgatcgtg tgcgacgagc
      301 ccgtgtcggc cctggatgtc tccatccagg cccagatcat caaccttctc gaggaccttc
      361 aggaccggct cggcctgacg taccttttca tcgcccacaa cctcagcgtc gtcaaacaca
      421 tcgcggaccg ggtcgcggtg atgtacctcg gcaagatcgt ggagatctcc tccacgaaga
      481 acgtcttcga ccacaccttg cacccctaca cggaagcgct gctttccgcc gtgccggtgc
      541 cggatactcg ggccgagcgc aagcgcgtga tcctgcgtgg cgaggtgccc agcccgagga
      601 atcccccgcc ggggtgccat ttccacacgc gctgccccta cgccatgccg atctgcagaa
      661 cggtcgaacc gaggctcgag gaccggggcg gggaccacct cgtgtcctgt cacctcccct
      721 ccctccagcc ggcctgacct cctcctcacg acgctcacgt tctccagggg ctcccgcaag
      781 gtccaacggg gacgcggcgg tgaccgagca ccgcatcgcc cggtccggcg gcccgtcagg
      841 cctcctggac cacctccatg gcgatccgcg cccagtccca gagccgttgg ggcttgtaac
      901 agacggtcga gatgtccttc atgatcacct cgatgggggc gccccgcgcc tgctcgagta
      961 ccgaagtcat ggccgcgcgt gccttctgcg gattccacgt ctcctcgacg aagatcgcgg
     1021 ggctcggctt gaagctcagc acgtagtcgc cgcctacctc gcgcaggatc cgttcggcat
     1081 cgttccacgg gctcatcgag atcttgcgga gattagggat cttcctcagc agcccgatct
     1141 tccggtccag cggctcgcag catccgtagt acgtcagccc ccatcgcttc aaccagcgaa
     1201 ggtcgtggcg gagcgcgaac tcccagtgca tctcgggaga gacctcggag aagatctgcg
     1261 cgttggagca gccccacatg ttggcgggcc tcacccggcc gggatcgaag tcggcgcctg
     1321 gcaggtccca cgtgtacccg tagcctccgg aaccgatgcg ggtattgctg cagtccaggg
     1381 ccagcaggtt ctgggcctcg aactggtcca gctcgatcat ccacgcgtcc accatccgtt
     1441 cgacggccgc gttgaccagc gccgggcgct cgaccagatc gatcatggcc tcctgcagac
     1501 cccaccaccg gatgaggtag tcccacggcg tgaaccagat gtgggactgc cccaccttgc
     1561 gcactgggat gatgtcccga aacagtccct gcatggcctg gaagacgacc tgcgtggtct
     1621 cctggtcgtg ggtgacgcgc ggcaggcgga tcttttcgat gtcctccggc tggcggatct
     1681 gcacgtggaa gtggcgggag acgatttcgt ttgcggcgtc cgtgcgcacc acgtccacgt
     1741 cttcgtggat gccgaagtcg gtgctgtgca cggcgagggg gcactccagg aagtcgctga
     1801 acaccatgtc cccgggcatg tggcgccact ggtacagcgt gcggcgaagc cgggtctcca
     1861 gctcccgggc ccaggggtga acggtccgga gggtgagctc gtcttccacg ttcatctcgt
     1921 gccacgggat ctcgttgatc cacaccatgg gacgggagga ctccaggtcg ttgagcttgc
     1981 gccagaggcc cgccttctcc cg
//
LOCUS       DASRZS010000586         7401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2642736, whole genome shotgun sequence.
ACCESSION   DASRZS010000586 DASRZS010000000
VERSION     DASRZS010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2642736"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1942)
                     /locus_tag="VFH83_14230"
     CDS             complement(<1..1942)
                     /locus_tag="VFH83_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6487580.1"
                     /translation="MGAGRSGSWRDRQSRRVSSVLILASLGTYVIVGLVTIYAHDWRT
                     VETVAVGAALLGIPHALLRRGHHRLGNLILMVIAVASVTVLATVGQGIRDLALLAFPI
                     IFIYVGLTSDRAMLVLCGVLTFLSLLWLALGQPLGWYAPVPLFPDPLNLFYLVVTTVL
                     LVTTTLAVDLLSSNLRKSLDQSRQEIEERKRVEEALRVSEAKFRAVVENSHDGILFTD
                     ENAVIHYRSPSYRRINGFSNDERIGRSGLETVHPQDLDAVHRNWAALLADPKTPRTFS
                     YRIRHKDGSWRWVETACLNLLHNPEVRSIVIASRDTTEHKQAEDALAASEERFRRMFE
                     RHSAAMIVIDPETGRLLEANQAASDFYGWSVDELTAMSIQDINTMSAEAVRREMETVR
                     SLGRTRFSFRHRRADGSARDVEVFSSSIELRGKAVLYSIVHDVTEQRKAEALLRQGEE
                     RYRLMFEYAPLAIVISRGTDVVYANPSCLKMLGLSCLEDLRRMPPLELFAPEARPQIQ
                     ENMVRRFQGPSVPASYETVCLRRDGTRFPILLYLAQAEFADGPAMLAFILDISERKRA
                     EEEQARLQDQLTQAQKMESVGRLAGGVAHDFNNMLGVILGHTDMALQQVPPEQPLRDS
                     LEEIRKASNHSADLTRQLLAFAR"
     gene            complement(1993..2244)
                     /locus_tag="VFH83_14235"
     CDS             complement(1993..2244)
                     /locus_tag="VFH83_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487581.1"
                     /translation="MNALVYINEPTKLPLQTVMQNLISQTRDLTKIVQQGNIGALRKL
                     SKKNVTDAAMIVSIVPILLAYPFLQRHFISGVVIGSIKE"
     gene            complement(2314..3084)
                     /locus_tag="VFH83_14240"
     CDS             complement(2314..3084)
                     /locus_tag="VFH83_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008028450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-threitol dehydrogenase"
                     /protein_id="HET6487582.1"
                     /translation="MEPSRVDADLSLAGSTALITGAAQGIGRAIAELFARKGASLVLV
                     DVKPEVRELGVLLARTPANALPIVADLTVTPELDRVVSEAERLTGRVDILVNNAGVAL
                     LGDALSLGEEAWDRTLALNLTAPFLLAQRVGRGMTRRGHGRIINIASQASVIGLEGHA
                     AYCASKAGLVGLARVLALEWARFGVTVNCISPTVTLTELGRKAWAGEVGEAMKRKIPA
                     QRFAEPEEVAAAALYLASSAADMINGTNLLIDGGYTAQ"
     gene            3434..4051
                     /locus_tag="VFH83_14245"
     CDS             3434..4051
                     /locus_tag="VFH83_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487583.1"
                     /translation="MTMPILAGAVAFGVLVLTDLAEPRSLLALRLASMALSALLLAYG
                     ILACALDPWKLGLSIAPRIVAGVVGAAFLYLLIRSLLLEVPTAKGQDAAAPKRLVTTG
                     TYALVRHPGVIWFLFVLLGAAAAADSAPLLVAAPIWAAADVIYAAVQDRIFFPRLFGD
                     AYGDYRRSTPFIIPNPASVRTCCETWNAPLDRSSMKPGGPNGNRV"
     gene            4035..5708
                     /locus_tag="VFH83_14250"
     CDS             4035..5708
                     /locus_tag="VFH83_14250"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HET6487584.1"
                     /translation="METAFEILNQSTQTPRTAGSTVTATELLRQGKKKEIWEQYCGFL
                     DLTTHGFMDLQRRLLAEQLPVLSASRLGAKLLRGETPKSIDEFRRTVPFTTYKDYASH
                     LLVKDESVLPEKPYAWVHTSGRSGEYDYKWVPYTRRMYELGGDAGLACLLLSAAKARG
                     EVALREGMTFPYLIAPPPYVSGIFTQRVLELFAFNTLPPLDKALTMDFQERTQEALAG
                     ALSEGLDFFFGVTSILIRISEQFSRLGGGGGGAALKMLLKPKALLRIARAFLKSRLMG
                     RPLLPKDIWKVQGAMCGGMDTSIFKDKVVESWGLVPLEAYASTEFGVMATQTWSRNGL
                     TFFPQCNLWEFILESDYRQLLEDPTFIPESHLMDEVKPNTEYVLAGTNLHGGALVRYI
                     GGDFVKITTLEDTEAGVRLPQMVFVSRIDDVIDVGSFTRLTEKTLWQAIENSGIPYED
                     WSVRKEYHGEQPVLHAYIELKEQSLEAEVVSERIRESLKKLDAPYRDLEELTGLKPFS
                     VTLLSKGTYRRYFEERQAAGADLAHLKPPHVNASDKMVENLMRMSSWKI"
     gene            5708..>7401
                     /locus_tag="VFH83_14255"
     CDS             5708..>7401
                     /locus_tag="VFH83_14255"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET6487585.1"
                     /translation="MLLFSTVVKLAALLSYLVLVLLILRSKAERDVRVFFSIYLFGML
                     FWQTTSVMVNFSQDAKTALLWYNLLLAGSGTFNILFFPFTRAFAKIKGQKFLTGFAYV
                     ACAALLGSGALGIQFQEVTLGKAGYWVPIFNRWSYVMALVSYFFWGFGISNLVRGLIH
                     EKSPVHRNRLVYVLLGGCVVLIGATSNLTFLQDYPFDICMNLASALIIGYAVIRFRLL
                     EIRVILVRSLFYSVLTVGGIAAYLGIVFGVERILKLGIGYSGPLYPIIAILVIAIVFL
                     PFRNGLQKGLDRVFFREKADYQNATQVFSRDITSLYDASDILSLVATTLSQTVKVGTL
                     TIAVLDAENQVFRAEKSIGSRTLAKSEVLFGVGSNLVQWLRHTGQPLVREEAELNPDS
                     RPIVEENRILFEIAGTSVVVPILLRDKLLGALNLGPKRAGTMYSRDDLRFLTTIANQT
                     ATALEKTSAYREIQRRLSEQTLLFILSERFRGTTDFDSIMQSVVRILKSFLACDYCAL
                     VYHETAAGSKVFALDPLSTVTATVVSEAQNNGGMAPNRGQDPFPLGEKLLGEIRRRS"
BASE COUNT         1246 a         2442 c         2365 g         1348 t
ORIGIN      
        1 ggcgcgcgaa ggccaggagc tggcgggtca ggtcggcgga gtggttgctt gccttgcgga
       61 tctcctccag gctgtcgcgc aagggttgct cgggggggac ctgctggagc gccatatcgg
      121 tatgccccag gatgaccccc agcatgttgt tgaagtcgtg cgccaccccg cccgctagcc
      181 ttcctacgga ttccatcttc tgcgcctggg tgagctggtc ctggagcctc gcctgttcct
      241 cctcggcccg cttgcgctcg gagatatcca ggatgaacgc aagcatcgcc ggcccgtcgg
      301 cgaactccgc ttgcgccaga tagaggagaa tggggaagcg ggtcccgtcc cgtcgcagac
      361 ataccgtctc gtagctggcc ggcacggacg gcccctggaa cctccgaacc atgttctcct
      421 gaatctgggg tcgggcctcc ggggcgaaca gctccaaagg cggcatgcgc cgcaggtcct
      481 cgaggcacga gagcccgagc atcttcagac agctcggatt ggcatagacc acgtcggtcc
      541 ctcgggagat gacgatggcc aggggcgcat actcgaacat caaccggtag cgctcctctc
      601 cctggcgcag gagcgcctcg gccttgcgct gctcggtgac gtcatggacg atcgagtaga
      661 ggaccgcctt cccccgcagc tcgatgctgc tgctgaagac ctcgacgtcc cgggcggagc
      721 cgtccgcgcg tcggtgtcgg aagctgaacc gggtgcgccc cagggagcga acggtctcca
      781 tctcccgccg cacggcctcc gcggacatcg tgttgatgtc ctggatgctc atcgcggtga
      841 gctcgtccac ggaccagccg tagaagtccg aggctgcctg gttggcctcc aggagccggc
      901 ccgtttccgg gtcgatgacg atcatggcgg cggagtgccg ctcgaacatc cggcggaagc
      961 gctcttcgct cgccgccaga gcatcctccg cctgtttgtg ctccgtggta tcgcggctgg
     1021 caatgacgat ggaccgcacc tcggggttgt ggagcaggtt cagacaggcg gtctcgaccc
     1081 atcgccagga tccgtccttg tgtcggatcc gataggaaaa cgtccggggc gtcttcggat
     1141 ccgccagaag ggcggcccag ttccggtgga ccgcgtccag gtcttgcgga tgtacggtct
     1201 ccaggcccga gcgtccgatg cgctcgtcgt tggagaatcc gttgatccgc cgataggaag
     1261 ggctccgata gtggatcacg gcgttctcgt ccgtgaacag gatcccatcg tggctgttct
     1321 cgaccacggc gcggaacttc gcttcactga cgcgaagcgc ctcctccacc cgcttgcgct
     1381 cctcgatctc ctgccgtgac tggtccaggg acttgcggag gttggaggag agcaggtcga
     1441 cggcaagggt cgtggtgacc aggaggaccg tggtgacgac gaggtagaag aggttgagcg
     1501 ggtccgggaa caggggcacc ggggcgtacc atcccaaggg ctgacccagg gcgagccaca
     1561 ggaggctgag gaacgtgagg acgccgcaga ggaccagcat ggcccggtcc gacgtgaggc
     1621 ccacatagat gaaaatgatg ggaaaggcca ggagcgccag gtcccggatc ccctggccga
     1681 cggtggccag caccgtaacg ctggccaccg cgatcaccat caggatcagg ttgcccagcc
     1741 gatggtgtcc gcggcgcagg agcgcgtgcg ggatgccgag cagggcggcg cccacggcga
     1801 ccgtttccac ggtcctccag tcatgggcgt agatcgtgac caggccgacg atcacgtagg
     1861 tacccagcga ggccaggatg aggacgctcg aaacgcgcct cgactgccgg tcccgccagc
     1921 ttccgctcct cccagccccc atgcccccct cctgcttggg aggaattcta ccccgcgccg
     1981 gcggttcccg ccttactctt tgatcgagcc gatgaccacg ccggagatga aatggcgctg
     2041 gaggaaggga taggccagga ggatcggcac aatggagacg atcatggcgg cgtccgtgac
     2101 gtttttcttc gagagcttgc gcagcgcgcc gatgttgccc tgctgcacga tcttggtgag
     2161 gtcccgcgtc tgggagatga ggttctgcat caccgtctgg agcgggagct ttgtgggctc
     2221 gttgatgtag accagcgcgt tcaccgagat ctgtctggcc ggcgcgcggc acgccgtgct
     2281 ccggttgccg agtcaggcga gagtatagcc gatctactgg gccgtgtatc ctccatcgat
     2341 gaggagattc gttccattaa tcatgtctgc cgcggagctg gcgagataga gcgcggccgc
     2401 ggctacctcc tcgggctcgg caaatcgctg ggcggggatc ttgcgcttca tcgcctctcc
     2461 cacctccccc gcccacgcct tgcggcccaa ctcggtgagc gtgacggtcg gggagatgca
     2521 attcacggtg acgccgaacc gggcccactc caaggcgagc acgcgggcca ggccgaccag
     2581 ccccgccttg ctggcgcagt aggccgcgtg cccctccagg ccgatcactg acgcctgaga
     2641 cgcgatgttg atgatcctgc cgtggccccg gcgggtcatg ccgcgtccca cgcgttgggc
     2701 aagcaggaac ggggccgtca ggttgagagc cagggtacgg tcccaggcct cctcgcccaa
     2761 cgagagagcg tctcccagga gggcgacccc cgcgttgttc accaggatgt cgacccgccc
     2821 ggttaggcgc tcggcttccg ataccacccg gtccaactcc ggcgttaccg tcaggtccgc
     2881 cacgatgggg agggcgttgg ccggcgttcg agccaacagg acgcccagct cccggacttc
     2941 cggcttgacg tccaccagga cgaggctcgc gcccttgcgg gcgaacagct ccgcgatggc
     3001 cctcccgatc ccctgggcgg ccccggtgat cagggccgta gatccggcga ggctgagatc
     3061 cgcatcgact ctgcttggtt ccatgatgtc tccctttgaa gggcggaccg gcgacaatcc
     3121 ggtcgttttc ctcccctccg cgtagcaaag ggtatcgacc ggctggtttc agggccaggg
     3181 tgccaggcaa gaacgccgcg ggggatggct ttttcggttc tggcggcaat tccgttgccc
     3241 cggaaattga aatgcgcttt ccgaggggtc atcattggga tgatcttgga gaccatgcgt
     3301 ctcgaggcgg gtagcgcaca aggtgggtag tgcaattgtg aaagacgggg cacacgacgc
     3361 gttcaaatcc gaacgggacc ggaaggcaac atgccagcca ggctcgcggc tcgagtgaca
     3421 gggaaaacgt accgtgacga tgccgatcct ggccggagcc gtggcattcg gggtccttgt
     3481 cctcaccgac ctcgccgagc cccggtctct acttgccctg agactcgcta gcatggcgct
     3541 ttccgcgctc cttctggcct acggaatcct ggcctgcgcc ctggacccgt ggaagctcgg
     3601 tctgtcgatc gcgcctcgga tcgtcgccgg ggtagtcggc gccgctttcc tctatctcct
     3661 catccggtca ctcctcctcg aggtcccgac ggcgaagggc caagacgccg cggccccgaa
     3721 gaggctcgtg accacgggga cgtatgccct ggtgcggcac cccggggtga tctggttcct
     3781 gttcgttctt ctgggagccg cggcggccgc tgattccgcg cctctgctgg ttgccgcccc
     3841 gatctgggcc gccgcggacg tgatctacgc ggcggtgcag gatcggatct tcttcccgcg
     3901 cctgttcggc gatgcctacg gggactatcg ccgttccact ccgttcatca ttcccaaccc
     3961 agcaagcgtg cggacctgtt gcgaaacctg gaatgcaccg ctcgatcgtt catccatgaa
     4021 accgggaggt cccaatggaa accgcgtttg agatcctcaa ccagtctacc cagacgcctc
     4081 ggacggccgg gagcacggtg accgccaccg agctgctgcg ccagggaaag aagaaggaga
     4141 tatgggagca atactgcggg ttcctggatc tcacgaccca tggcttcatg gatctgcaac
     4201 ggaggctcct cgctgaacag cttccggtgc tttcggcgtc tcggctgggg gcaaagctcc
     4261 tgagagggga gaccccgaag tccatcgacg agttccgccg cacggtaccc ttcaccacat
     4321 acaaggacta tgcgtcccac ctcctggtga aggacgagtc ggtgctcccg gagaagccgt
     4381 acgcgtgggt ccacacctcg ggccgaagcg gcgagtacga ctacaagtgg gtcccctaca
     4441 cccgacggat gtacgagctg ggcggtgatg ctggacttgc ctgcctgctc ctttccgcgg
     4501 cgaaagcgcg cggggaggtg gcgctgaggg aaggcatgac cttcccgtat ctgatcgcgc
     4561 ctccgcccta cgtttccggt atcttcaccc agcgggtcct ggagcttttc gcgttcaaca
     4621 cgcttccccc cctggacaag gcgctcacca tggacttcca ggagcggacc caggaggcgc
     4681 tggccggcgc cctgagcgag ggtctggact tcttcttcgg cgtaacctcc atcttgatcc
     4741 ggatcagcga gcagttcagc cgcctgggcg gcggcggcgg gggcgccgcc ctgaagatgc
     4801 tcctcaagcc caaggccctc ctgcggatcg cccgcgcctt cctgaagagc cgcctcatgg
     4861 gccgaccgct tctcccgaag gatatctgga aggtgcaggg cgccatgtgc ggcggcatgg
     4921 acacctccat cttcaaggac aaggtcgtgg agtcctgggg cctcgtgccc ttggaagcgt
     4981 acgcgtccac ggagttcggc gtcatggcca cccagacctg gagccgcaac ggcctcacgt
     5041 tcttccccca gtgcaacctg tgggagttca tcctggagtc cgactatcgc cagctcctgg
     5101 aggatcccac cttcatcccc gagtcccacc tgatggacga ggtgaaaccc aacacggaat
     5161 acgtgctcgc cggcacgaac ctgcacggag gagccctggt gcgctacatc ggtggagact
     5221 tcgtgaagat caccaccctg gaggacacgg aggcgggggt ccgcctgccc cagatggtgt
     5281 tcgtctcccg gatcgacgat gtgatcgacg tcgggtcgtt cacccgcctc accgagaaga
     5341 ccctctggca ggccatcgag aactccggca tcccctacga ggactggtcg gtccgcaaag
     5401 agtaccacgg tgagcagccc gtgcttcacg cgtacatcga gctcaaggag caatcgctgg
     5461 aggcggaggt ggtctccgag cggatccggg agagcctgaa gaagctcgat gccccctatc
     5521 gggacttgga ggagctcacc ggcctcaagc cgttcagcgt gacgctcctt tccaagggga
     5581 cctatcgtcg ttactttgaa gaacggcagg cggcgggggc ggatctggct cacctgaagc
     5641 ctccccacgt gaacgcatcg gacaagatgg tggagaacct gatgcgcatg agctcctgga
     5701 agatctgatg ctcctcttct cgaccgtcgt caagctcgcg gccctcctca gctacctcgt
     5761 gctggtgctg ctcatcctgc gcagcaaagc ggagcgggac gtacgggtct tcttctcgat
     5821 ctatctgttc gggatgctgt tctggcagac cacctcggtg atggtcaatt tctcccagga
     5881 cgcaaagacc gcgctcctct ggtacaacct cctcctggcg ggctcgggca cgttcaacat
     5941 cctgttcttc cccttcacgc gagcattcgc gaagatcaag ggccagaagt tcctgaccgg
     6001 tttcgcctac gtggcctgcg cggccctgct ggggagtggc gccctgggca tccagttcca
     6061 ggaggtcacc ctcggcaagg cggggtattg ggtacccatc ttcaaccggt ggagctacgt
     6121 gatggccctg gtcagctact tcttctgggg cttcggcatc tccaacctgg tccgaggcct
     6181 gatccacgag aagtctcccg tccatcgcaa ccgcctggtc tatgtccttc tgggcggctg
     6241 cgtcgtgctg atcggcgcca cctccaacct cacgttcctc caggactacc ccttcgacat
     6301 ctgcatgaac ctggccagcg cgctcatcat cgggtacgcc gtgatccggt tccggctgct
     6361 ggagatccgg gtcatcctgg tgaggagcct gttctactcc gtcctgaccg taggggggat
     6421 cgccgcctac ctcggcatcg tcttcggtgt ggagcgcatc ctcaagctcg gcatcggcta
     6481 ctcgggcccg ctgtacccca tcatcgccat cctggtgatc gccatcgtct ttctcccctt
     6541 ccgcaacggc ctccagaagg gcctggaccg ggtcttcttc cgcgagaagg cggactacca
     6601 gaacgccacc caggtcttca gccgggacat cacgagcctg tacgacgcat cggacatcct
     6661 ctccctggtg gccaccaccc tttcccagac cgtgaaggtc gggacgctca ccatcgccgt
     6721 gctggatgcg gagaaccagg tcttccgcgc cgaaaagtcc atcggctccc ggacgctggc
     6781 caaaagcgag gtcctgttcg gcgtagggag caacctggtg cagtggctgc gccacacggg
     6841 ccagccgctg gttcgggagg aagccgagct caatccggac agccggccga tcgtggagga
     6901 gaaccggatc ctcttcgaga ttgccgggac ctccgtggtg gttccgatcc tcctgcggga
     6961 caagctgctg ggcgcgctga acctgggtcc caagcgggcg gggaccatgt acagccggga
     7021 cgacctgcgg ttcctcacca ccatcgccaa ccagacggcc acggccctgg agaagacctc
     7081 ggcctaccgc gagatccagc gccggctgtc cgaacagacg ctcctcttca tcctgagcga
     7141 gcggttccgc ggcaccaccg acttcgactc catcatgcag tccgtggtgc ggatcctgaa
     7201 gagcttcctg gcctgtgact actgcgccct ggtctaccac gagaccgccg cggggtcgaa
     7261 ggtcttcgcg ctggaccccc tcagcacggt gaccgccacc gttgtctcgg aggcgcagaa
     7321 caacggcggc atggcgccga accggggaca ggacccgttc cccctcgggg agaagctcct
     7381 cggcgagatc cggcgccgct c
//
LOCUS       DASRZS010000587         7309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1490304, whole genome shotgun sequence.
ACCESSION   DASRZS010000587 DASRZS010000000
VERSION     DASRZS010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490304"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="VFH83_14260"
     CDS             complement(<1..1182)
                     /locus_tag="VFH83_14260"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET6487586.1"
                     /translation="MTRHPRSLLGGATALAIAGLLWAAAPALADITIPTQPRPPGPPN
                     LDLLKPIEDILSSKPALIVMGKDPRVQASTFGEGEAGGGDAGGSDSTSGASTAAAAKP
                     SPVLQAAGGFLVPFRNPAPAFSRGILITRDYSDFPVQTEPHIAVDPADPDHVVVATID
                     YNSPSVTDYVTYDGGVTWEGPFPTGYLPEDEATGGDPVLDFDRKGNLYLATISIGTEE
                     FTVGPEYTSSIVSSIAVAQSKDGGYTWPSIISTARSNVSITGQQIDAQGHLRGSVQIG
                     FFDKPWISIGRNPAKPDQDVIYAVYVEFITYYDIIYTGELPILLPREVSSTIKLVHSE
                     DQGTTWSDPIAVSPVVRRAYGTTPGVALPGVENNDRTLQGPRVEAAMDGTVYVAWLDT
                     TD"
     gene            complement(1218..1811)
                     /locus_tag="VFH83_14265"
     CDS             complement(1218..1811)
                     /locus_tag="VFH83_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487587.1"
                     /translation="MIMRNGSGGRARPVGTLLASAAMLLLLVPPVWSSGTSEQTAGGS
                     ASAGAAPGAAPQAAGHHSLQSLGTTDLQAFINGMEADRLLLAEIRKDLPDKRADADLY
                     LKRLKDLAGQSDPVHLVPKVNRLIEQEPIYYDWLEKNGTAQGQNQGQSASEYVVGGAR
                     GFVVAFQDFQNALLLTVINRLDIAERALQEATLAPSK"
     gene            complement(1852..3174)
                     /locus_tag="VFH83_14270"
     CDS             complement(1852..3174)
                     /locus_tag="VFH83_14270"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6487588.1"
                     /translation="MRILRDPRVVFVSGFLLVALQVLDAQQAPAAGQTPAGSSGQPAP
                     ASPAAAAPSQPAITIGVTDVLRDFDSAWQTIKDNFVDPKTYGVDWDALKTQYRPQVAA
                     APDPASAYKLFAEMLDKLKSPNTGVTPPWAKAAAADANSSLNEPLLQYGGVGMLLQPL
                     QSGDVFVLQVFKGTPADKAGVLVGDVITGVDDWRVAGDDAMNQVTQRVRGVVGTVVKL
                     TLRDPEGKERTLEMPRAQIDLRPSVDYRTVSGTIGYMRLPALSKDLVDAASKALPSLL
                     STNGLVLDLRSVSSGTLDQMIVVAQWFLGAASMGGLVGHQGGEAFPFNQNAVAAYQRP
                     MVVLTNSNTYGIAEILAYILRDYKRAGIVGQKSAGQFEVADEVTLPSGGLLDYAVGRF
                     VTAQGDLMPLEGLTPDVSVDPPDLATLRAGRDIVIEKAVDTLRSNPRW"
     gene            complement(3289..3819)
                     /locus_tag="VFH83_14275"
     CDS             complement(3289..3819)
                     /locus_tag="VFH83_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487589.1"
                     /translation="MKKVVLMLVGLLLTGAAAPTWSLPTGLATQAPRWYEAEFHVAWN
                     LARRAPMVGCRFAFALGSPVSLGTSFYISGDGGVATVDAAVDYLPAGLPADLSLPLTV
                     GFGFARKGGSLGLALGATAGVSWYPFTLSHPTVDAWLLGLGGCVEASFYLLPWGIEPL
                     FVTGVMVPVNLSVMGD"
     gene            complement(3885..4892)
                     /locus_tag="VFH83_14280"
     CDS             complement(3885..4892)
                     /locus_tag="VFH83_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487590.1"
                     /translation="MSSEAARPLSRTAGKGPVSWFLRRYALQLGVVGVAMVVWLLFLA
                     GSPRTFLAKEIYISFMSTTPFFALMAIPITLVVITGEIDLSFGSVMAFGMMAYDVMFK
                     ATGSAWLGFVACLIAGLAAGVFNGVIVVRIGVPSLVATIGTQFLLRGAVLIVTNGQGL
                     GMTEVTSTFLHTALVGRAFGVIPMQFFWTIAVGVIIWFFLNRHRYGAHVYLTGDNIES
                     ARLMGVNVGRTKIVSFAVVGLLAAFAGFVVSAEVQFFWPTLGDGYLLNTLASIFLGGT
                     SVFGGTGTIFGTFVASFVIGAINAGIVAAGLTGFWTNFIYGLIIVGSVSIQTVVTRRL
                     S"
     gene            complement(4903..5664)
                     /locus_tag="VFH83_14285"
     CDS             complement(4903..5664)
                     /locus_tag="VFH83_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6487591.1"
                     /translation="MAHLLDMRNISKTFGEVAAVHDVNFHVGANEIVGLLGDNGAGKS
                     TLVKIITGYHRPDAGGEILWQGNRLDHLSVARAHQLGIEVVYQERALADKQSLWRNIF
                     MGRELTFPGGRLNVKGMRAETRKLMHSAMGFTSSAVTPESTVRTFSGGERQGVAITRA
                     LYFKAELVILDEPTMGLSLSETKKALDFVEGIRTAGKAAIFIDHNIFHVYPVVDRIVV
                     LDRGTVAGEFRKNELSLEELTERLYAVAKTGTLKG"
     gene            complement(5745..6737)
                     /locus_tag="VFH83_14290"
     CDS             complement(5745..6737)
                     /locus_tag="VFH83_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6487592.1"
                     /translation="MKSRKALAIAGVVLALAMVAQVATAQTKWASGVRIVFFPGGPQG
                     GVFAVNVYNGAKQAEADLGVHVDYVWSDWDPQKMIQQFQEAVATRPDGICIMGHPGDA
                     AFDALIDQAEAKGIIVTSQNTTLDKAEGKYAANGFGYVGAENYSAGHALGAEALKRSG
                     LKKGDRAMVWGLLSQPARGERTKGVIDAFKEGGLIVDYLDIDSATNADPVAGVPTFAG
                     YVSSHPDVKMVVTDHGGLTATAETYLKAAGKKPGDIYLAGFDLSPATITAIRGGWTDL
                     VIDQQEWLQGYLPILQICLSKVYGFSGLHINTGAGFVDKSNVEFVAPLAQKNIR"
     gene            complement(6790..>7309)
                     /locus_tag="VFH83_14295"
     CDS             complement(6790..>7309)
                     /locus_tag="VFH83_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6487593.1"
                     /translation="EMGGKGSVAVLQGGLAATNLNLRTLGFKTALARQAPGIQVVEVA
                     DEGGDFALSVSKTEDLLSRYPSLSAIFSVSAEGAPAAAAVLKQRGRSGRVLVAGFDDL
                     SDTLQGIRDGTISFCIVQNTYRMGWLSVERLLDAVSGRPVPRVIDTGVVFVDRRNLDS
                     YRRSMEEGNAHP"
BASE COUNT         1224 a         2439 c         2419 g         1227 t
ORIGIN      
        1 gtccgtggta tccagccagg caacgtagac ggtgccgtcc atcgccgctt ccacgcgggg
       61 tccctggagg gttcggtcgt tgttctccac gcccggcaac gcgactcccg gggtggtccc
      121 gtaggcgcgc cgcacgaccg ggctcacggc gatcgggtcg ctccaggtgg ttccctggtc
      181 ctcggagtgg accagcttga tggtgctgga aacctcccga ggcagcagga tgggaagctc
      241 gccggtgtag atgatgtcgt agtacgtgat gaactccacg tacacggcat agatcacgtc
      301 ctggtccggc ttcgcggggt tcctaccgat gctgatccag ggcttgtcga agaagccgat
      361 ctgcaccgag ccgcgaagat gaccctgggc gtcgatctgc tgcccggtga tggagacgtt
      421 gcttcgggcc gtggagatga tgctgggcca cgtgtagccg ccgtccttgg actgggccac
      481 ggcgatgctc gacacgatgg agctcgtgta ttccggcccc accgtgaact cttcggtgcc
      541 gatggagatc gtggcgagat acaggttccc cttccggtcg aagtccaaca ccgggtcacc
      601 gccggttgcc tcgtcctccg gcagataccc ggtcgggaag ggtccttccc aggtcacccc
      661 gccgtcatag gtgacgtagt cggtgaccga tggcgagttg tagtcgatgg tggccaccac
      721 cacgtggtcc gggtcggccg ggtccaccgc gatgtgaggc tcggtctgga ccgggaaatc
      781 gctgtagtcc cgggtgatga ggatgccacg gctgaacgcg ggagccgggt tgcggaacgg
      841 aaccaggaac ccacccgccg cctggagcac cggcgagggc ttcgccgcgg cagccgtgga
      901 ggcgccggaa gtggagtccg agccaccggc gtctccgccg ccggcttcgc cctcaccaaa
      961 ggtgctggcc tggactcggg ggtccttgcc catgacgatc agcgcgggct tgctcgagag
     1021 gatgtcctcg atgggcttga ggaggtccag attagggggg ccgggaggcc gaggctgcgt
     1081 gggaatcgtg atgtccgcca gcgcaggggc ggctgcccac agaagaccgg cgatcgccag
     1141 agccgtggcc ccgcccaaca gggacctggg gtgtcttgtc atggtggttt gtattccttt
     1201 cctctgttca tttcatccta tttcgacggt gcgagcgtgg cctcctggag cgcgcgctcg
     1261 gcaatgtcca ggcggttgat cacggtgagc aggagggcgt tctggaagtc ctggaacgca
     1321 accacgaacc cgcgcgcgcc gcccacgacg tactccgacg cgctctggcc ctggttctgc
     1381 ccttgcgcgg tgccgttctt ctccagccag tcgtagtaga tcggctcctg ctcaatgaga
     1441 cggttgacct tggggaccag gtggaccggg tcggactgcc cggcgaggtc cttcaggcgc
     1501 ttgagataca ggtccgcgtc ggctcgcttg tccggcaggt ccttgcggat ctccgccagg
     1561 aggaggcggt ccgcctccat gccgttgatg aatgcctgca ggtccgtagt gcccagcgac
     1621 tgcagggagt gatgcccggc ggcctggggc gcagcgcccg gggcggcccc ggcagacgca
     1681 gagccgccgg cggtctgttc cgaggttccg ctgctccaga ccggcgggac aagcagaagg
     1741 agcatcgcgg cggaggccag cagggtcccc accgggcgcg cgcgcccgcc actcccgttg
     1801 cgcatgatca cggcatgctc ccttccgtgg tcccgtgtcc ccgacccgag gctaccaccg
     1861 cgggttggag cgcagggtgt ccaccgcttt ctcgatgacg atgtcccggc ctgcgcgcag
     1921 cgtcgccaga tccggcggat ccacgctcac gtccggggtg agcccttcca ggggcatgag
     1981 gtctccctgg gcggtaacga atcgtcccac cgcgtagtcc aggagtccgc cgctgggcag
     2041 ggtgacctcg tcggccacct cgaactggcc ggcggacttc tggccgacga taccggcgcg
     2101 tttgtagtcc cgaaggatgt aagccaggat ctccgcgata ccgtaggtgt tcgagttggt
     2161 gagcaccacc atgggtctct ggtaggcggc aaccgcgttc tggttgaacg ggaacgcctc
     2221 ccctccctgg tgccccacca ggccgcccat cgaggcggcg cccaggaacc actgcgccac
     2281 cacgatcatc tggtccaggg tgcccgagct gaccgaccga agatccagga cgagcccgtt
     2341 ggtggacagg agagagggaa gagccttgct ggccgcgtcc accaggtctt tgctcaacgc
     2401 cggcagccgc atatacccga tggttccgga cacggtgcgg taatccacgc tggggcgcag
     2461 gtcgatctgg gccctcggca tctccagcgt ccgctccttc ccctcggggt cgcgcagcgt
     2521 gagcttcacc acggtcccca ccacgccgcg cacccgctgg gtgacctggt tcatggcgtc
     2581 gtccccggcg acccgccaat cgtcgacgcc ggtgatgacg tcacccacca ggaccccggc
     2641 cttgtcggcg ggagtgccct tgaacacctg gaggacgaac acgtctcccg actggagggg
     2701 ctgcaggagc attcctactc ccccgtactg caggagcggc tcgttgaggc tgctgttggc
     2761 atccgcggcg gcggcctttg cccacggcgg tgtcacaccg gtgtttgggc tcttgagctt
     2821 gtccagcatc tccgcgaaga gcttgtacgc ggaggccggg tccggcgcgg ccgccacctg
     2881 gggacggtac tgcgtcttca gcgcatccca gtccacgccg taggtcttgg gatcaacgaa
     2941 gttgtccttg atggtctgcc aggccgaatc gaagtcccgc agaacgtcgg tgacgccgat
     3001 cgtgatggct ggttgggacg gcgcagccgc agccggggac gcgggagccg gctggcccga
     3061 ggagccggcc ggagtctggc cggccgcggg cgcctgctga gcgtccagca cctggagcgc
     3121 cacgagcagg aagcccgaga caaacaccac cctggggtca cggagtattc gcattcagcc
     3181 ccctctgacg cgccgaccgg ctcactgcgc cgcgaatact accaaagtaa ggatgcgatt
     3241 gcgttcgtgc aagtgtaatg ggtacggggc tcgccttgcc atctcggact agtcgcccat
     3301 gaccgagagg ttgaccggga ccatcacgcc ggtcacgaag agtggctcga ttccccaggg
     3361 gagcagatag aagctcgctt cgacgcatcc acccagaccc aggagccatg catccacggt
     3421 cggatggctg agcgtgaacg ggtaccagga aaccccggcg gttgctccga gcgccagtcc
     3481 gagactcccg cccttgcggg cgaatccgaa tccgacggtc aggggcagag agagatcggc
     3541 cgggagcccg gcgggaaggt agtccaccgc cgcgtcaacc gtggcgaccc cgccatcgcc
     3601 cgagatgtag aagctggtcc ccaggctgac cggcgagccc aaggcgaagg cgaatcgaca
     3661 ccccaccatc ggagcgcgcc gagccaggtt ccaggcgacg tggaactccg cttcatacca
     3721 tcgcggcgcc tgggtcgcga gcccggtggg caggctccac gtcggcgccg cggcgcccgt
     3781 cagcaggagc cccacgagca tcagcacgac tttcttcatg ctcggacctc ctcggtcggc
     3841 gtcgcgctgc ccgaatcgac ccggcggccg ctgtctcttg ctctctagct cagcctccgc
     3901 gtaaccacgg tctggatgga caccgacccc acgatgatga ggccgtagat gaaattggtc
     3961 cagaacccgg tgaggcccgc cgccacgatg cccgcgttga tggcgccgat gacgaaggaa
     4021 gccacaaagg tgccgaagat ggtcccggtg ccgccgaaca cggaggtgcc ccccaggaaa
     4081 atggaggcca gggtgttgag caggtaccca tcccccagcg tgggccagaa gaactggacc
     4141 tcggcgctca ccacgaatcc ggcgaaagcg gccagcaatc ccacgaccgc aaaggagacg
     4201 atcttggtcc tgccgacgtt cacccccatg agtcgggcgc tctcgatgtt gtcccccgtg
     4261 agatagacgt gcgcgccgta gcggtggcgg ttcaggaaga accagatgat gaccccgacg
     4321 gcgatggtcc agaagaactg catagggatc acaccgaagg ccctgccgac cagcgcggtg
     4381 tgcaggaacg tggaggtcac ctcggtcatg cccaggccct ggccgttggt cacgatcagg
     4441 accgcgccgc ggagcaggaa ctgcgtgccg atggtggcca ccagagacgg cactccgatg
     4501 cgtacgacga tgacgccgtt gaacactcca gccgccagac ccgcgatgag gcaggccacg
     4561 aatcccaacc aggcgctccc cgtggccttg aacatcacgt cataggccat catgccaaag
     4621 gccatcaccg acccgaagga gaggtcgatc tccccggtga tgaccaccag ggtgatcggg
     4681 atggccataa gggcgaaaaa cggcgtcgtg gacatgaagg agatgtagat ctccttggcc
     4741 aggaatgtgc ggggtgaacc ggccaggaag aggagccaga ccaccattgc aaccccgacc
     4801 acgccgagct gcagggcata ccttcggagg aaccaggaaa ccgggccctt gccggccgtt
     4861 ctggatagag ggcgcgccgc ctcgctgctc attcgtgtct cctcagccct tcagagtgcc
     4921 ggtcttggcc acggcgtaca ggcgctcggt cagctcttcc agcgagagct cgttcttgcg
     4981 gaactcgccg gccacggtgc cccgatccag caccacgatc cggtccacca ccgggtagac
     5041 gtggaagatg ttgtggtcga tgaagatggc ggccttgccg gccgtgcgga tcccctcgac
     5101 gaagtccagc gcctttttgg tctccgaaag ggacaggccc atcgtgggct cgtccaggat
     5161 gaccagctcc gccttgaagt acagggcgcg ggtgatggcg acgccctggc gctctcctcc
     5221 ggagaacgtc ctcaccgtgg actccggggt gaccgcggag gaggtgaagc ccatggcgga
     5281 gtgcatcagc ttgcgggtct cggcccgcat ccccttgacg ttcagcctgc cgccggggaa
     5341 ggtcagctcc cgccccatga agatgttgcg ccacagggac tgcttgtccg ccagggcgcg
     5401 ctcctggtag accacctcga tcccgagctg gtgcgcccgc gccaccgaca ggtgatccag
     5461 gcgattgccc tgccagagga tctccccgcc ggcatccggg cgatgatacc cggtgatgat
     5521 cttcaccagg gtggacttgc cggcgccgtt gtctcccagg agacccacga tctcgttggc
     5581 gcccacgtgg aagttcacgt cgtggaccgc cgcgacctcg ccgaaggtct tggagatgtt
     5641 cctcatgtcc agcaggtgtg ccatggcttc ccctcagaga gacggcctgg cgcggccgcg
     5701 tcggccgcgc caggcaggat gatggtccgc aaaccgctcc gggactagcg gatgttcttc
     5761 tgcgccagag gcgccacgaa ctctacattc gatttgtcca cgaatccagc cccggtgttg
     5821 atgtgcagac cggagaaccc gtagaccttg ctcaggcaga tctggaggat gggcaggtag
     5881 ccctggagcc actcctgctg gtcgatgacc agatcggtcc acccgccgcg gatggcggtg
     5941 atggttgccg gagagagatc gaagccggcc aggtagatgt ccccgggctt cttgccggcg
     6001 gccttcaggt acgtttccgc cgtggcggtg agaccgccgt ggtccgtgac gaccatcttg
     6061 acgtccggat gggacgagac atagccggcg aacgtgggca cccccgctac cgggtccgcg
     6121 ttggtcgcgg aatcgatgtc gaggtagtcc acgatcagac caccttcctt gaaggcatcg
     6181 atgacgccct tggtgcgctc accgcgcgcg ggctgggaga gcagccccca taccatggcg
     6241 cggtcgcctt tcttgagccc ggaccgcttg agcgcctcgg cgcccagggc gtgtcccgcg
     6301 gagtagttct cggcgcccac gtacccgaac ccgttggcgg cgtacttgcc ctccgccttg
     6361 tccagcgtgg tgttctggct ggtcacgatg atccccttgg cctcggcctg gtcgatcagc
     6421 gcgtcgaacg ccgcgtctcc cgggtggccc atgatgcaga tcccgtcggg cctggtggcc
     6481 acggcttcct ggaactgctg gatcatcttc tgcggatccc agtcggacca cacgtagtcc
     6541 acgtgcacgc ccaggtccgc ctccgcctgc ttggcgccgt tgtacacgtt gacggcgaag
     6601 acgcctccct gcggcccgcc cgggaagaac acgatgcgga cccccgaggc ccacttggtc
     6661 tgcgcggtgg ccacctgggc caccatcgcc agagccagca ctacgcctgc gatcgcgagc
     6721 gcttttctgg acttcatacg cgaaaccccc ctcaccgaga tttgcaggcc ccaacggggc
     6781 cttggaatcc taaggatgcg cgttgccctc ctccatgctg cgccgataac tgtccaggtt
     6841 ccggcgatcc acgaacacga cgcccgtatc gatgactctg gggaccggac ggccgctgac
     6901 ggcgtccagc aggcgttcga cggacagcca gcccatgcgg taggtgttct ggacgatgca
     6961 gaacgagatc gttccgtccc ggatgccctg gagcgtgtcg ctgagatcgt cgaaccccgc
     7021 caccaggact cggcccgacc ggccgcgctg cttgaggacc gccgccgccg cgggcgcccc
     7081 ctccgcggac acgctgaaga tcgcgctcag ggacgggtag cgggagagaa ggtcctccgt
     7141 cttgctcacg gacagcgcga aatccccgcc ctcgtcggca acctccacca cctggatgcc
     7201 cggcgcctgt ctggcaagcg cggtcttgaa ccccagcgtc cggaggttga ggttcgtggc
     7261 tgcgagaccg ccctggagga cggctacgga gcccttgcct cccatctcc
//
LOCUS       DASRZS010000588         4889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3394103, whole genome shotgun sequence.
ACCESSION   DASRZS010000588 DASRZS010000000
VERSION     DASRZS010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3394103"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VFH83_14300"
     CDS             <1..704
                     /locus_tag="VFH83_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007458155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6487594.1"
                     /translation="VFDEKTRQVTGEDASARYIRKACAASLRRLQTDYIDLYQFHNNG
                     YPAEEAPAVRDALEDLVKEGKIRAYGWSTDFPDRAEVFATGPHCASIQFQLNVLTDNA
                     AVLSVCEKHGLAAINRGPLAMGLLTGKYTAESRPADDDVRGVKAPEWMGFFKNGMPGP
                     DWLRKRDAVRQVLESGGRTLAQGALGWLWARSSRTIPIPGFRTAAQVEENAAALSKGP
                     LSPDQMAEIFRILQS"
     gene            764..1663
                     /locus_tag="VFH83_14305"
     CDS             764..1663
                     /locus_tag="VFH83_14305"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET6487595.1"
                     /translation="MDPAVLGPHVRWVCAQNGFSCRSILPGTAGTFPTFVVDRRVVVK
                     LFGPLFEGRLCWRVEREAAELVRSIPSFPAAGVLAFGRLADPGWRYIVYRFVQGRSIG
                     QARRRVSHTDLRALARRLGSWVRDLHALEVGARTALPRLTARKLASWYAAKRARGFPG
                     WPAHLAAQADAFLSAAGLSASPITHLIHGDLTADHVLGELRHGRWRTSGVIDFGDCMR
                     GDLFYELGALHLDLFDADKRLLSVFLSSYRVSREDQEGFERKAMARAICHRFDVLGPT
                     FARKPRLRRLDSLDEVAAALWQV"
     gene            complement(1699..3714)
                     /locus_tag="VFH83_14310"
     CDS             complement(1699..3714)
                     /locus_tag="VFH83_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487596.1"
                     /translation="MTPGDGVIELFYPRRLASWWTVLEDILYPEKRIRDKILRRAEGL
                     AEARIDSVVQFGFHTRFDFIPYFGALHAYHAAVAEELHRFGIRFIEHFSCNVIARPRD
                     AADRERYHRAQRHHVALYPDLVARPTLAYEGYRVDSLRSVTVTTGLPSYAAQYQCELL
                     CHANPDFRAMTQAYLRRYLSEVPVDGIEVDDMAIYDGFLSCGCQHCRERFRRETGLEL
                     PPVDDRRFWGDTSRSPLLWGNYDNPDFRAWVQMRLAATSEHFGLIRQTVGSHLRLTTC
                     SSNTGPILMNAFGMRYESLAAQVNLILLENNGIDVSAADWPRTEPEAMLQKSYAQSRG
                     GLPVVALSYANCADGAYLGWAIARFWGVSNWTSTLVQGLAEDPPGLLESSQYVAAFNR
                     WDLENGDLDVREIAPATEVYLAYSRHGKENGLRLPDGRDVWERISQWSRALLARNVSY
                     QFIREDTLGDAMERLEAPVILESCACLSDRQADGIIEHARRGGRLILRGPLGTRDENG
                     RDRERRVMEELMALGPPPGVTVVGSGEPGELVDGLIHQGRIRPAIRASDPSSGYVARL
                     HAYAGRAVLHLLNTLLEGVPDPRTTDEWATANAMLSGIRSLDPTPRLVLEIDFSLLGC
                     AVWRNPRLRSPEVTGDLPLGFRRLDAGRCRLDVDLSRLRLYAMVAEA"
     gene            complement(3722..>4889)
                     /locus_tag="VFH83_14315"
     CDS             complement(3722..>4889)
                     /locus_tag="VFH83_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487597.1"
                     /translation="QPGGSGGGGASPGIDPLDPAGFDTLWEAVAARRHEPAFADGVLR
                     QHGIRAYVTSIENRADAPPRPPVHPQDVDLAFSLHPESWSMFDCHPLIWPERATDAGL
                     FLQGHRFQAERYLLHLQEYFQQEIADADQLQDAVRSFFLKVLRSPRTNPRSRILYVNC
                     FQAEDWRFSGPWSRAVVDDAIRNHREQLDGERRRQVILCVAEAMLEALNRIGKEYQDQ
                     GERFGCCLQMCGGATHFMDWERQVQSLPAPIPRLAQDEYPVWARYPHVHFEYICAHEG
                     LYRDLSNAAKQVANVSVGPWWHLFRRKRIAAMISDQLSMGSAAAIACGFTDARFVEMI
                     GAKYQSLRRAVVDAVAELVDDPASALHGDVDGAAALMRDLLFTNPAAVHHIPLG"
BASE COUNT          787 a         1625 c         1677 g          800 t
ORIGIN      
        1 ccgtgttcga cgagaagacc cggcaggtta ccggggaaga cgcatccgcc cggtacattc
       61 gaaaggcctg cgcggcgtcc ctccgtcgcc ttcagaccga ttacatcgac ctctaccagt
      121 tccacaacaa cggctatccc gcggaggagg cgcccgcggt tcgggacgcg ctggaggacc
      181 tcgtcaagga aggcaagatc cgcgcgtacg gctggagcac cgactttccg gatcgggccg
      241 aggtcttcgc gaccgggccc cactgcgcat cgatccagtt tcagctcaac gtcctcacgg
      301 acaacgcggc cgtcctaagc gtgtgcgaga agcacggcct ggcggccatc aaccgtggtc
      361 cccttgccat gggcctgttg acgggcaagt acaccgccga gtcgcgccct gcggatgacg
      421 acgttcgcgg cgtcaaggct ccggagtgga tgggcttctt caagaacggg atgccgggac
      481 cggactggct gcgcaagcgt gacgcggtcc gccaggtcct ggagagcggc gggcgcaccc
      541 tcgctcaggg ggcgctggga tggctgtggg cgcggagctc caggaccatc cccatcccgg
      601 gattccgaac ggcggcccag gtcgaggaga atgccgctgc gctctccaag ggcccgttgt
      661 ccccggacca gatggcggag atcttccgca tcctgcagag ctagcggtta cggccggtcg
      721 ggtgcgcggg ctcgagagtc tggagggcta ccgccgccgt ttcatggacc ccgcggtcct
      781 cggaccccac gtgcggtggg tgtgcgctca gaacggattc tcctgccggt ccatcctacc
      841 ggggacagcg ggcacgttcc caacgttcgt cgttgaccgt cgcgtggtgg tgaagctttt
      901 cgggcccctg ttcgagggcc ggctctgctg gcgggtcgag agggaggccg cggagctggt
      961 gcgcagcatt ccctcgtttc ccgccgcggg cgtgctcgca ttcgggcgac ttgcggaccc
     1021 gggctggcgg tatatcgtgt accgattcgt gcagggccgg agcatcggcc aggcgcggcg
     1081 cagggtgtcg cacaccgacc tgcgcgccct ggcgcgcagg ctcgggtcat gggtccggga
     1141 tctgcatgcg cttgaggttg gggcccgaac ggcccttccc agactcacgg cgcgcaagct
     1201 cgcgagctgg tatgcggcga agcgggcgag agggtttccg ggctggcccg cccacctggc
     1261 cgcgcaggcc gacgccttcc tctccgccgc ggggctgagc gcgtcgccga tcacccacct
     1321 catccacggg gacctgaccg cggaccacgt cctgggggag ctaaggcatg gcaggtggcg
     1381 gaccagcggg gtgatcgact ttggcgattg catgcgcggg gatctcttct atgagctcgg
     1441 tgcgctgcac ctggacctgt tcgacgcgga caagagactc ttgtccgttt tcctgagctc
     1501 ctaccgggtc tcccgtgagg accaggaagg cttcgagcgg aaggcgatgg cccgagccat
     1561 ctgtcatcgg ttcgatgtgt tgggcccgac gtttgcgcga aagccacgtc tccgccgtct
     1621 ggacagtctg gacgaggtgg cggctgccct ttggcaggtt tgagtcgttg ggatcgtcct
     1681 cggtgccgtc ggcgtccttc aggcctcagc gaccatggcg tagagcctga ggcgcgagag
     1741 atcgacgtcg agcctgcatc ggccagcatc cagtcggcga aaacccagag gaaggtctcc
     1801 cgtcacctcg gggctcctga gcctcgggtt cctccagacc gcgcacccca gcagggagaa
     1861 gtcgatctcc agcaccagcc ggggagtcgg gtccagcgat cgaatgccgc tcagcatcgc
     1921 gttcgccgtt gcccactcgt ccgtggtcct cggatccggg acgccctcca ggagcgtgtt
     1981 gaggaggtga aggaccgcgc ggcccgcgta ggcgtgcaac cgagcgacgt agccgctgct
     2041 ggggtctgac gcgcggatgg ccggccgaat cctcccttga tggatcaggc catccacgag
     2101 ctcccctggc tctcccgatc cgaccaccgt caccccaggc ggcggaccca gcgccatcag
     2161 ctcttccatg acccgtctct cgcggtctct gccgttctca tcgcgcgttc ccagtggtcc
     2221 cctgaggatg agccggccgc cgcggcgcgc atgctcgatg atcccatccg cctgccgatc
     2281 cgacaggcag gcgcaggatt ccaggatgac cggcgcctcg agcctctcca tcgcatcgcc
     2341 cagcgtgtcc tcacggatga actggtagga gacgtttcgg gccagcaggg ccctggacca
     2401 ctgagagatc cgctcccaga cgtcccgacc atccggcaga cgcagtccat tctccttccc
     2461 gtggcgggag tacgccaggt acacctccgt cgccggcgcg atctccctca cgtccaggtc
     2521 cccgttctcg aggtcccatc ggttgaaggc cgcgacgtac tgcgacgatt ccaggagtcc
     2581 gggcgggtcc tccgccagcc cctggacgag cgtgctcgtc cagttggata cgccccagaa
     2641 cctggcgatg gcccaaccca ggtacgctcc atcggcgcag ttggcgtagc tcagggcgac
     2701 caccggcagc ccgccgcggc tctgagcgta ggacttctgc agcatggcct ccggctccgt
     2761 cctcggccag tccgcggcgc tcacgtcgat gccgttgttc tccagcagga tgaggttgac
     2821 ctgggcggcc agggattcgt agcgcatgcc aaaggcgttc atcaggatgg ggccggtgtt
     2881 cgacgagcag gtggtcagcc gcagatgact gcccaccgtc tggcggatca gcccgaagtg
     2941 ctccgaggtg gcggccaacc gcatctgtac ccacgcgcgg aagtccggat tgtcatagtt
     3001 gccccagagg agcggagacc gcgaggtgtc tccccagaag cggcggtcat cgaccggcgg
     3061 aagctccagt ccggtctccc gtcggaagcg ctctcgacag tgttggcatc cgcagctcag
     3121 gaagccgtcg tagatcgcca tgtcatccac ctcgatgccg tccaccggga cctcggagag
     3181 gtagcgtcga aggtaggcct gggtcatggc tcggaagtcc gggttggcgt gacagaggag
     3241 ctcgcactgg tactgggccg cgtaggacgg cagaccggtc gtgacggtga cggaccgcag
     3301 ggagtcgacc cggtatccct cgtaggcgag ggtaggccgc gcaacgaggt ccgggtataa
     3361 cgcaacgtgg tgccgctggg cgcgatggta gcgctcacgg tccgccgcgt cgcgcggccg
     3421 ggcaatgacg ttgcaggaga agtgctcgat gaagcggatc ccgaaccggt ggagctcctc
     3481 cgccacggcc gcatggtaag cgtgaagggc gccgaaatac ggaatgaagt cgaatcgggt
     3541 gtggaatccg aactgaacca ccgagtcgat cctggcctcg gcgagcccct ccgcccgacg
     3601 gagaatcttg tcgcggattc tcttctcggg gtagaggatg tcctccagga ccgtccacca
     3661 gcttgccagc cggcgggggt agaacagctc gatcacgccg tctccaggag tcacgacgag
     3721 ctcatcccaa cgggatgtga tgcaccgccg cggggttggt gaacagcagg tcgcgcatca
     3781 gggcggccgc cccgtccacg tccccgtgca aggccgatgc gggatcgtcc accagctcgg
     3841 ctacggcatc caccaccgcg cgacggaggg actggtactt ggcgccgatc atctcgacga
     3901 accgtgcgtc cgtgaacccg cacgcaatcg cggcagccga gcccatgctg agctggtccg
     3961 agatcatggc ggcgatccgc ttccgccgga acagatgcca ccagggaccc acggagacgt
     4021 tggctacctg cttggccgcg ttcgagagat cgcggtacag gccctcgtgg gcgcagatgt
     4081 actcgaagtg gacgtggggg tagcgggccc agaccgggta ctcgtcctgc gcgaggcgcg
     4141 ggatgggcgc cgggagcgac tggacctgcc gctcccagtc catgaagtgc gtagcgccgc
     4201 cgcacatctg caggcagcac ccgaaccgct cgccctgatc ctggtactcc ttgccgatgc
     4261 ggttcagcgc ctccaacatc gcctccgcca cgcagaggat cacctgcctt cgcctctcgc
     4321 cgtcgagctg ctcgcggtgg ttgcggatgg catcgtccac gacggcgcgg gaccagggac
     4381 cgctgaagcg ccagtcctcg gcctggaagc agttgacgta gaggatccgg ctccgcgggt
     4441 tggtgcgggg gcttcggagg accttgagaa agaacgaccg caccgcatcc tggagctggt
     4501 cggcgtcggc gatctcctgc tggaaatact cctggagatg caggaggtag cgttccgcct
     4561 ggaaccgatg gccctgcagg aacaggccgg cgtccgtggc ccgttccggc cagatgagcg
     4621 gatggcagtc gaacatggac caggactcag gatgcagcga gaacgccagg tccacgtcct
     4681 gggggtgcac gggaggacgg ggcggagcgt ccgcccggtt ctcgatgctg gtcacgtacg
     4741 cccggatccc gtgctggcgg aggacgccgt ccgcgaaagc gggctcgtga cgtcgggccg
     4801 ccaccgcctc ccacagcgtg tcaaagcccg cgggatccag ggggtcgatc ccgggggagg
     4861 cgccgccgcc gcccgaacca ccgggctgc
//
LOCUS       DASRZS010000589         2696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5712364, whole genome shotgun sequence.
ACCESSION   DASRZS010000589 DASRZS010000000
VERSION     DASRZS010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5712364"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..414
                     /locus_tag="VFH83_14320"
     CDS             145..414
                     /locus_tag="VFH83_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487598.1"
                     /translation="MIFLVSQFLFVLFVLVGLIIWASSEMPLAWREVAINTRKNGETG
                     YGYIGLKVLSVCLKIMAVLVWILGIVALVMINAGSNLGGLLPSGL"
     gene            complement(461..1762)
                     /locus_tag="VFH83_14325"
     CDS             complement(461..1762)
                     /locus_tag="VFH83_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HET6487599.1"
                     /translation="MGWRDRLEVYLREKLYPFWFERVAAPEGGFTTYFDRNGKPTGQT
                     DKTLIQQTRSIFMLSHAIRNGYDETDPGQPARAGAGGRPRARALLEQGLPWFLERFRD
                     PVFDGWFWIVDKTGKPVSTDKIMYGQSFVIYCMSECALATGDPRAREAAEHTFDLAQK
                     YAADTAFGGYREMFLRDWRPRPAGAYGGDRKSFDVHMHLMEAYTTLYELTRADIHRRK
                     LLEVIEVLWARMMNRATSTGIAQFALDFTPLPAILFKTVWGSDRDNEGAPRPLNNTSY
                     GHNIELLWLFLHALEVLGEKVDPWRDRLAALADHTLRYGVDPQYGGMYVEGAHDGPAR
                     DTQKEFWQQAESLVGLLDCYLLFREPRYWEAFKKVAGFVWTHMINHEVGEWFALLDRD
                     GTVRWDYLGHAWKNNYHTTRSMVQTLRRLRRIEEAGLPTPE"
     gene            1863..>2696
                     /locus_tag="VFH83_14330"
     CDS             1863..>2696
                     /locus_tag="VFH83_14330"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET6487600.1"
                     /translation="MGNASRSSGGSDPRVIGIQGSPHTDGMTQRLVERALAGAAREGA
                     RTDLVRLADEELTPCEACGGDCFGTQVCVRDRTATIRHARLQDADGVVFGAPVYCWQL
                     NGLSSLFIDKMRWDTGSLLSPRNHRVAFGIACAGGSGTGCVIAVQALYRYFTNWAFHC
                     VEPLPVTRFNFDEALATAEERGAGMVRTLRSGVQPFASMGQALAHHQALPYMQWGPIE
                     ELALIVDQIRCGLLGSQDPQAVEFVRQAAAATAPAPSAARAEALSAAYFAGVEVWGRI
                     GR"
BASE COUNT          414 a          871 c          919 g          492 t
ORIGIN      
        1 cgagagtagc aggattggag accttggtga agcgcccgaa cgccacgcca tgcgccaggc
       61 gccgcgtcga acgccggcac ttttttcttg acagccggtt gcggggtgct agacttctcc
      121 ctgagacgct tcggaggtga aacgatgatc ttcctggtct ctcagttcct gttcgtgctg
      181 ttcgtcctgg tgggactcat catctgggcg agctccgaga tgccgttggc ctggagggaa
      241 gtggccatca atactcgcaa gaacggggag acgggctacg ggtacatcgg cctgaaggtg
      301 ctttcggtgt gcctgaagat catggccgtt ctcgtctgga tcctggggat cgtggccctc
      361 gtcatgatca acgcgggctc gaacctgggg ggactcctgc cgtccgggct gtagccggcc
      421 gtcccaccag ggctctttcc gagggccctt cggccccagc ttactccggc gtgggcagcc
      481 ccgcctcttc gatcctccgc aatctgcgca gcgtctgaac catcgaacgc gtcgtgtgat
      541 agttgttctt ccacgcgtgg cccaggtagt cccagcggac cgtgccgtcc cggtccagga
      601 gcgcgaacca ctctccgacc tcgtggttga tcatatgggt ccagacaaat ccggcaacct
      661 tcttgaaggc ttcccagtac cggggctccc ggaagaggag gtagcagtcc agcaaaccca
      721 ccagggactc cgcctgctgc cagaactctt tctgggtgtc ccgtgccggc ccgtcatggg
      781 caccttccac gtacatgccg ccgtactgcg ggtccacgcc gtagcgcagg gtgtggtccg
      841 ccagggccgc aagccggtcc cgccagggat ccaccttctc tccgaggacc tccagcgcgt
      901 gcaggaaaag ccagaggagc tcgatgttgt ggccgtagct ggtgttgttc agcggtcggg
      961 gcgctccttc gttgtcccga tcggaccccc agacggtctt gaacaggatc gccggtaacg
     1021 gcgtgaaatc aagggcgaac tgggcgatcc ccgtggaggt ggcccggttc atcatccgag
     1081 cccaaagcac ctcgatcacc tccaggagct tgcggcggtg gatgtcggcc cgggtcagct
     1141 cgtacagcgt cgtgtaggcc tccatgaggt gcatgtgcac atcgaagctc ttgcggtctc
     1201 cgccataggc gccggcgggg cgaggccgcc agtccctcag gaacatctcg cgatagccgc
     1261 cgaacgccgt gtccgccgcg tatttctgcg cgaggtcgaa ggtgtgctcc gcggcctcgc
     1321 gggcgcgggg gtctccggtg gccagggcgc actcgctcat gcagtagatg acgaagctct
     1381 gcccgtacat gatcttgtcc gtggatacgg gcttccccgt cttgtccacg atccagaacc
     1441 atccgtcgaa caccggatct cggaacctct ccaggaacca cggcagcccc tgttccagga
     1501 gcgccctggc gcgcggacgc ccgcccgcgc ccgcgcgcgc gggctgtccg gggtcggtct
     1561 cgtcgtagcc gtttcgaatg gcatgggaga gcatgaagat ggaccgcgtc tgctggatca
     1621 gggtcttgtc ggtctggccg gtaggcttgc cgttgcggtc gaagtaggtg gtgaagccgc
     1681 cctccggcgc cgcaacgcgc tcgaaccaga acggatagag cttctccctg aggtatactt
     1741 ccagacgatc tcgccacccc atgtgggtac ctccgtcggg ggccgtgacc ccggcgggtt
     1801 gttatagctc ggcgggtgga cacgcgccaa ggtgccgcat acgatgggag gaggaggacg
     1861 ggatggggaa cgcgtccagg tcgagcgggg gatccgaccc gcgggtcatc ggcatacagg
     1921 gcagccctca tacggatgga atgacccagc ggctcgtgga gcgcgcgctg gccggcgccg
     1981 cccgagaggg cgcccggacg gacctggtcc gattggcgga cgaggagctg acgccgtgcg
     2041 aggcctgcgg gggtgactgt ttcggcaccc aggtctgcgt ccgcgacagg accgccacga
     2101 tccgccatgc gcgcctgcag gacgcggacg gcgtggtctt cggcgctccc gtttattgct
     2161 ggcagctcaa cggcctgtct tcgctcttca tcgacaagat gcgatgggac accggttccc
     2221 tgctctcccc ccgcaaccac cgcgtggcct tcgggatcgc gtgcgccggg ggaagcggca
     2281 ccggctgcgt catcgcggtc caggcgctgt accgctactt caccaactgg gcgtttcact
     2341 gcgtagagcc gctgccggtg acccggttca acttcgatga ggcgctggcc acggcggagg
     2401 aacgcggtgc cgggatggtg cggacgctgc gctccggcgt ccagcccttt gccagcatgg
     2461 ggcaggccct ggcgcaccac caggctctgc cttacatgca atggggaccg atcgaggagc
     2521 tggcgctgat cgtggatcag atccgctgcg ggcttctggg gagtcaggat ccgcaggcgg
     2581 tcgagttcgt gcggcaggcc gcggcggcaa ccgcgccggc accgtcagcg gcgcgcgccg
     2641 aggcgctttc cgccgcgtat ttcgccggtg tcgaggtgtg gggccggatc ggccgg
//
LOCUS       DASRZS010000590         7327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4946991, whole genome shotgun sequence.
ACCESSION   DASRZS010000590 DASRZS010000000
VERSION     DASRZS010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4946991"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1192)
                     /gene="metK"
                     /locus_tag="VFH83_14335"
     CDS             complement(35..1192)
                     /gene="metK"
                     /locus_tag="VFH83_14335"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET6487601.1"
                     /translation="MAGDYQFTSESVNEGHPDKVCDQIADAVLDSLLAQDPDSRVALE
                     CMITTGTVHVAGEITTAGFADVQKIARSVLQEVGYTNPDFGIDCFDAGVWVSIHEQSR
                     EIADGVSGAAGHPQGAGDQGIMFGYACDETPERMPLPIMLAHRLCMRLSQVRRDRLIP
                     DLGPDGKSQVTVEYRKDRPTRVASVVVAQQHGRSLVESRLRQQIIDKVVKPVCGPLFD
                     DGTRVHVNAAGTFTVGGPEADTGVTGRKVVADTYGGMARCGGGALSGKDPSKVDRSGT
                     YMARAIAKTIVAAGLARRCEVQLAYVIGEPGPENLSIETFSTGSVDERKLEALVRGCF
                     PLTPQEIIRALDLKRPIYRRTACFGHFGREEPQFTWERTDRAALLRAQAGL"
     gene            complement(1192..2799)
                     /locus_tag="VFH83_14340"
     CDS             complement(1192..2799)
                     /locus_tag="VFH83_14340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6487602.1"
                     /translation="MEGAPGSRSLDPSIFIAIVEDGPTSVVITDPQGSIEYVNRKFTT
                     VTGYNRQEVIGRNPRILKSGVQAAEVYSDLWRTILEGREWRGELCNRRKSGELYWELA
                     AISAIRDANGSVTHFLAIKEDITDLKTMEQLLWQAKANAEAANRAKSRFLADMSHEIR
                     TPLNSILGFAQLMEMQASGALTNKQKEYVRWIREGGEHLMDMVNDVLDLSKVEAGRVE
                     LEKASVDVGALVNRVITITRSLTAKKHLHLETAVGPEVGVISADEVRLKQVLYNLLSN
                     AIKFTPDGKRIGVEAARDEENVAITVWDEGIGISAAVQARIFEPYAQAPKSGEGTGLG
                     LAIVRRLVELHGGSVRVESSQGSGSRFTVRLPVGALPAGALSAGAPPAGARPANVPEV
                     AAAGSADSTRPSRPRHPVPLRPTRDFRGKTALVVDDNRANRVLMERVLTTLGCGVVFA
                     ESGEQALEVARQSPCDIVFMDVRLPGMSGGEAMHELRRSGVKAPIIAVTAHAMKGDGE
                     KFLSEGFSAYIPKPVYISRILEALEKL"
     gene            complement(2802..3794)
                     /locus_tag="VFH83_14345"
     CDS             complement(2802..3794)
                     /locus_tag="VFH83_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6487603.1"
                     /translation="MNPIIRKLTECFGPSGHEAQVRELIRSELPRGAQVRVDPMGSLI
                     ARQKGSGKGRRVMLAAHMDEIGVVVTHVDQKGFLRFGAVGGVRPLPLMGGRVQFANGT
                     IGVIGVERIEDASRVPALEKFYIDVGASSPADCPVKVGDVACFLRTCEEQGKRIFAKA
                     MDDRIGCAVLLMALKELERSPHDISFVFTVQEEVGLRGAATSGYGEEPEIAIAVDVTL
                     TGDTPECPPMAVSLGAGPAVKIKDGGMLSHVGVKDWLIRTAESAGIPYQREVLVAGTT
                     DAAAIQVSRAGVPAGCLSIPTRYVHMPSEVVDSGDVDGAVKLLVSALNGPIDIG"
     gene            complement(3791..4840)
                     /locus_tag="VFH83_14350"
     CDS             complement(3791..4840)
                     /locus_tag="VFH83_14350"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET6487604.1"
                     /translation="MLLQRLSDAFGVSGAEQEVRSILLAEIRDRVDACRIDALGNLIA
                     IKKASGSPSGRILLAAHMDEVGLMVTTFDDNGLVRFAKVGGIDDRVLPARGVLVGEKR
                     VPGVIGVKPVHLTEKSDRDQVMSWKQLAIDLGATTRSEAERLVQRGDRAVVATEFREL
                     AREGSSWRTVQGKALDDRVGCALLAELLRHRYAFDLVAAFTVQEEVGLRGAGVAAYAE
                     KPDAAIALECTGANEVPTKKDVNPSTRLGGGPAITIQDASFIADARLVRHATATAEAQ
                     GIPHQYKQPTIGGTDAGAMQRTRAGVPAITIAVPARYIHSPAAIMNLNDFDATLTLLR
                     EMLARMPVAELRSEP"
     gene            complement(4843..5952)
                     /locus_tag="VFH83_14355"
     CDS             complement(4843..5952)
                     /locus_tag="VFH83_14355"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6487605.1"
                     /translation="METTSLLRELAEARGPSGYEAEIRDRVRRSFGERADEVRVDAVG
                     NLIALRRGEGPEPRPSIMLAGHMDEIALMVSQIEKGFLRVAQIGGFDPRVLLGQEVVV
                     HGTRELDGLVVSVPPHFTDAAEREKPVPLEKLFVDVGLPPSEVETLVRVGDLVTLRGR
                     FLTLAGGYAACKSLDDRVAVAAVTLCLEELSRRRRTWDVYAVATTQEEVGLKGAVTSG
                     YGIRPTVAIAIDATFGIQPGLSPAETQKMDAGPSIALGPNIHPAVYDRLVAAAKAIEL
                     PYQVEALPGSTGTDAWALQVSRSGLPCGLVSIPVRSMHTPVETVCLRDIERTARLLAE
                     LISRLDDAFAGQLETRDALSAPPAASAPPDPKGKV"
     gene            complement(5984..6991)
                     /locus_tag="VFH83_14360"
     CDS             complement(5984..6991)
                     /locus_tag="VFH83_14360"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6487606.1"
                     /translation="MSSVPAPASTDHRQQRVALVQNLVAILTWSLAPSMVRAVTGSFP
                     VNFQNACRYITALLVLWPVYLLSARRDRPKMRSDLRQLLSRAPRILVIALAAYGFQVC
                     YTYSLFLITPSLQALVGQVQVIFAVLFAVLLFPDERAFIRGWRFLAGVTAALGGVALV
                     IVGGRAFGTVEFGVGVLVVVASNLCWALLGSLLRAWVPDLPAQMTVSAVFTVVSPAFV
                     VTYAIAHHGLPIPAAPLTHWLLLVSSGLVAIGLGHSMFYRAVPVLGVSVSTSLGLLLP
                     LLVSLVSFVALGETLTSLQLVGGVILLAGSFLVIRARFRGGPARAPGAVPGARAANRA
                     P"
     gene            complement(7005..>7327)
                     /locus_tag="VFH83_14365"
     CDS             complement(7005..>7327)
                     /locus_tag="VFH83_14365"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET6487607.1"
                     /translation="VGNRLVYTIYAYRYFPGWHFFTVHDAKATKDQGIRALQEGWGFQ
                     GWPLVVFGDDVNDMPMFRIASRAVAVRNAIPEVKALAHEVIGTNEEDSVLRWISADHA
                     KGRG"
BASE COUNT         1179 a         2517 c         2460 g         1171 t
ORIGIN      
        1 gagaaggtag tggggtttca cgcggactcc tggactacag gccggcctgc gcccgcaaga
       61 gggccgcacg gtcggtccgt tcccacgtga actgcggctc ttcgcgaccg aaatggccga
      121 agcaggccgt ccttcggtaa atggggcgct tcaggtccag cgcgcggatg atctcctgag
      181 gagtcagggg gaagcagcct cgcaccagcg cttccagctt gcgctcgtcc acggagccgg
      241 tggagaacgt ctcgatgctg aggttctcgg ggccgggctc tccgatcacg tacgcaagct
      301 gcacctcgca gcggcgggcc aggccagcag cgacgatggt cttggcgatg gcgcgcgcca
      361 tataggtgcc ggatcggtcc accttgctgg ggtccttgcc ggaaagggcg ccgccgccgc
      421 atcgcgccat gccgccatag gtgtccgcca ccaccttgcg gccggtcacg cccgtgtccg
      481 cctcgggacc gccgacggtg aacgtgcccg cggcgttgac gtggacccgg gtgccgtcgt
      541 cgaagagagg accgcagacc ggcttcacta ccttgtcgat gatctgctga cgcagccggc
      601 tctctaccag gctccttccg tgctgctggg ccaccaccac ggacgccacc cgggtgggcc
      661 ggtcctttcg atactccacc gtgacctggc tcttgccgtc cggtcccagg tccgggatga
      721 gccgatcccg ccgaacctgg gagagccgca tgcacaggcg atgagccagc atgatgggga
      781 gcggcatccg ctcgggggtt tcgtcgcagg cgtagccgaa catgatgccc tggtctcccg
      841 caccctgggg atgaccggcc gcgccgctca cgccgtcggc gatctcccgg ctctgctcgt
      901 ggatggagac ccacaccccg gcgtcgaagc agtcgatgcc gaaatccggg ttggtgtagc
      961 ccacctcctg gagcaccgag cgcgcaatct tctgcacgtc ggcgaacccc gcggtggtga
     1021 tctctccggc cacgtggacg gtcccggtcg tgatcatgca ctccagcgcc acgcgggagt
     1081 ccggatcctg ggccaggagg gaatccagca ccgcgtccgc gatctggtca cagaccttgt
     1141 ccggatgtcc ttcgttgacg gattccgagg tgaactggta gtcgccggcc atcacagctt
     1201 ctccagcgct tcgaggattc gggagatgta taccggtttg gggatgtagg cgctgaaacc
     1261 ctccgagagg aacttctccc cgtcgccctt catggcatga gcggtcaccg caatgatcgg
     1321 ggccttcact ccggagcgcc gtagctcgtg catcgcctca ccgccgctca tccccggaag
     1381 acgaacgtcc atgaacacga tgtcgcacgg ggactgccga gccacctcga gcgcctgctc
     1441 gcctgactcg gcgaacacca cgccgcaccc cagggtggtc agcacgcgct ccatcaggac
     1501 ccggttggcc cggttgtcgt cgaccaccag cgccgttttg ccccggaaat cccgggtggg
     1561 ccggagcgga acgggatggc gcggtcggga cggcctcgtg ctgtcggccg agccggccgc
     1621 agccacctcc gggacgttcg ccgggcgcgc tcccgcggga ggcgctccag cagaaagcgc
     1681 gcccgcgggg agcgccccca ccggaagccg tacggtgaag cggctgccgg atccctggct
     1741 gctctccacc cgcacggatc cgccatgcag ctcgaccagg cgacgaacga tcgccaggcc
     1801 caaccccgta ccctctccgc ttttcggcgc ctgggcgtag ggctcgaaga tcctggcctg
     1861 gaccgccgcg gagatgccga tgccctcgtc ccacacggtg atggcgacat tctcctcgtc
     1921 ccgtgccgcc tccacgccga tgcgtttccc gtccggggtg aacttgatgg cgttggaaag
     1981 caggttgtac aggacctgct tgagacgcac ctcatcggcg gagatcacgc ctacttcagg
     2041 accgacggcg gtctccaggt gaaggtgctt cttggcggtg agggaacgcg tgatcgtgat
     2101 tacgcggttg acgagcgcgc ccacgtccac ggacgccttc tccagctcga cgcgcccggc
     2161 ctccaccttg gagaggtcca ggacgtcgtt gaccatgtcc atgaggtgct cgccgccctc
     2221 gcggatccag cggacgtact ccttctgctt gttggtcagc gcgccgctgg cctgcatctc
     2281 catgagctgg gcgaacccca ggatggagtt gagaggcgtg cggatctcgt gactcatgtc
     2341 cgccagaaac cggcttttgg cgcggttggc cgcctcggcg ttggcctttg cctgccagag
     2401 gagctgttcc atggtcttca ggtccgtgat gtcttccttg atggccagga agtgcgttac
     2461 ggatccgttt gcgtcccgga tggcggagat ggcggccagc tcccagtaca gctcgccgct
     2521 cttgcgccgg ttacagagct caccgcgcca ctcccgtccc tccaggatgg tgcgccagag
     2581 gtccgagtag acttcagcgg cctgcacccc ggacttgagg atgcgcgggt tgcggccaat
     2641 cacttcctgc cggttgtagc cggtgaccgt ggtaaacttg cggttcacgt actcgatgct
     2701 gccctggggg tcggtgatca ccaccgaagt gggaccgtcc tccacaatgg cgatgaagat
     2761 gctcgggtcg agagaccggg agcctggtgc gccttccacg gtcagccgat gtcgatggga
     2821 ccgttcaggg cgcttaccag cagcttgacg gctccgtcca cgtcgccgga gtccaccacc
     2881 tcggaaggca tgtggacgta gcgggtaggt atggacaggc acccggcggg gacgcccgcc
     2941 cggctgacct ggatggccgc ggcgtccgtg gtccccgcta ccaggacctc ccgctggtac
     3001 gggatgccgg cgctctccgc cgttcggatg agccagtctt tgacgccgac gtggctgagc
     3061 atccccccgt ccttgatctt gacggccgga cccgcgccaa ggctcaccgc catgggtggg
     3121 cactccggag tgtctcccgt gagcgtgacg tccacggcta tggcgatctc gggctcctcg
     3181 ccgtatccgg aggtcgcggc tccccggagg cccacctcct cctggacggt gaagacgaag
     3241 gagatgtcgt ggggagagcg ctccagctcc ttcagggcca tcaggagcac cgcgcacccg
     3301 atgcggtcgt ccatggcctt ggcgaagatc cgcttgccct gctcctcaca ggtgcgcagg
     3361 aagcaggcca cgtcccccac cttcacggga cagtccgcgg ggctgctcgc cccgacgtcg
     3421 atgtagaact tctccagggc aggcacccgg gaggcgtctt caatcctctc gacgccgatc
     3481 acccctatgg tcccgttggc gaactgaacc cttccgccca tcaaggggag cgggcgaacg
     3541 ccccccacgg ccccaaatcg gaggaacccc ttctggtcca cgtgggtcac cacgacgccg
     3601 atctcgtcca tgtgagccgc cagcatcacc cgccgtccct tgccggatcc cttctgtcgg
     3661 gcgatcaggc tccccatggg gtccacgcgc acctgcgcgc cgcggggcag ctcggagcgg
     3721 atgagctcac gcacctgtgc ctcgtgcccc gagggaccga agcattcggt gagcttgcgg
     3781 atgattggat tcacggttca ctccttagct ctgccacggg catccgggcc agcatctctc
     3841 tcagcagcgt aagcgtggcg tcgaagtcgt tcaggttcat gatcgccgcc ggagagtgga
     3901 tgtagcgcgc aggcacagcg atggtgatgg cggggacacc ggcgcgcgtg cgctgcatgg
     3961 cccccgcgtc cgtccctccg atggtcggct gcttgtactg atgggggatg ccctgggcct
     4021 ccgcggtggc cgtggcgtgg cgaaccagcc gggcatcggc gatgaagctc gcgtcctgga
     4081 tcgtgatggc cggaccgccg cccaggcgcg tggacggatt gacatccttc ttcgtcggga
     4141 cctcgttcgc gcccgtgcac tccagcgcta tcgcggcgtc gggcttctcg gcgtaggcgg
     4201 ccacgccggc cccgcgcagt ccgacctcct cctggaccgt gaacgccgcg accaggtcga
     4261 acgcgtagcg gtggcgcaag agctcggcca ggagggcgca ccccacgcga tcatccaggg
     4321 ctttgccctg gaccgtgcgc caggaggatc cttcccgggc cagctctcgg aactccgtgg
     4381 caaccacggc acggtctcca cgctggacca gccgttccgc ttcggagcgc gtggtggcgc
     4441 ccaggtcgat ggcgagctgc ttccagctca tgacctggtc ccgatcggac ttttccgtca
     4501 aatgcaccgg tttcacgccg atgacgccgg gcacccgctt ctctcccacc aggacgccgc
     4561 gcgcgggaag gacccggtcg tcgatcccac cgaccttggc gaagcgaacc aggccgttgt
     4621 cgtcgaaggt ggtcaccatg agccccacct cgtccatgtg ggcggcaagg aggatgcggc
     4681 cgctggggga tccgctcgcc ttcttgatgg cgatgagatt gcccagggca tcgattctgc
     4741 aggcgtccac gcgatctcgg atctcagcca ggaggatcga acggacttcc tgctcggcac
     4801 cggagacgcc gaaggcatcg gagagacgct gcagcagcat ggtcagacct tgcctttcgg
     4861 gtccggtgga gcggacgcag ccggcggcgc cgacagggcg tcgcgggtct cgagctgccc
     4921 ggcgaaagcg tcgtccaggc ggctgatgag ctccgccagc agacgggccg tgcgctcgat
     4981 gtcgcggagg cagaccgtct ccaccggcgt atgcatggag cgaaccggga tgctgaccag
     5041 gccgcacggc agacccgacc ggctcacctg cagcgcccag gcgtccgttc ccgtcgagcc
     5101 gggcagcgcc tcgacctggt acgggagctc gatggccttg gccgccgcca ccaggcgatc
     5161 gtagacggcg gggtgaatgt tcggccccag ggcgatgctg ggtcccgcgt ccatcttctg
     5221 ggtctccgcg ggagagagcc ccggctggat gccgaaggtc gcatcgatgg cgatggccac
     5281 ggtcggtcgg ataccgtagc cggaggtgac ggcgcccttc agccctacct cttcctgggt
     5341 cgtggccacc gcatagacgt cccatgtcct tcggcgccga ctcagctctt ccaggcagag
     5401 ggtgaccgcc gcaaccgcaa cccggtcatc cagggacttg caggccgcgt agcccccggc
     5461 cagcgtcagg aaccggcccc gaagcgtcac caggtctccc acgcggacca gggtctcgac
     5521 ctccgagggg ggaagaccga cgtccacgaa gagcttctcc agggggacgg gcttctcccg
     5581 ctccgcggca tcggtgaagt ggggaggcac gctgaccacc aggccatcca gctcccgcgt
     5641 cccgtggacc acgacctcct ggcccaggag caccctcgga tcgaaaccgc cgatctgggc
     5701 cacgcggagg aatcccttct cgatctggct gaccatgagc gcgatctcgt ccatgtgacc
     5761 ggcgagcatg atcgagggtc ggggctcggg gccctctccc cgacgcaggg cgatgaggtt
     5821 ccccaccgcg tccacgcgca cctcgtcggc gcgctcaccg aaggagcgcc gcacgcggtc
     5881 gcgtatctcg gcctcgtacc ccgacgggcc gcgcgcctcg gcgagctccc tcaggagact
     5941 ggtggtttcc atgggcagaa gataccagcg ggggctgcgc agctcaaggc gctcggttgg
     6001 cggcacgcgc gccgggcacg gccccgggcg ctcgcgccgg cccgccgcgg aaccgagcgc
     6061 ggatcacaag gaaggatccg gcgagcagga tcaccccacc cacgagctga agcgacgtga
     6121 gcgtctctcc gagcgcgaca aaggagacca gggagaccag gagcggcagc aggagcccga
     6181 ggctcgtcga caccgagacg cctagcaccg gcacggcgcg gtagaacatg gagtgcccca
     6241 ggcctatggc caccaggccc gaggagacga ggagcagcca gtgggtgagt ggagcggccg
     6301 gaatgggtag gccgtggtgc gcgatcgcgt aggtcacgac gaacgccggg ctcacgacgg
     6361 tgaacaccgc ggagacggtc atctgggcgg ggaggtccgg cacccaggcc cgaagcaagg
     6421 agcccagaag ggcccagcag aggttgcttg ccaccaccac cagcacgccg acgccgaact
     6481 ccacggttcc gaacgcccgt ccgcccacga tcaccagagc cacgcctccc agcgccgccg
     6541 tcactccggc caggaaccgc cagcctcgga tgaaggcgcg ctcgtcgggg aaaagcagca
     6601 ccgcgaagag gacggcaaag atcacctgga cctggcccac cagcgcctgc agggacgggg
     6661 tgatcaggaa gaggctgtag gtgtagcaga cctggaagcc gtaggccgcc agcgcgatga
     6721 ccaggatccg gggcgcccgg gacaggagct ggcggagatc cgatctcatc ttcggccggt
     6781 cgcgccgcgc gctgagcaga tacacaggcc atagcaccag aagggcggtg atgtaccggc
     6841 aagcgttctg gaagttcacg gggaaggagc cggtgaccgc gcggaccatg gagggcgcca
     6901 gggaccaggt gaggatcgcc accaggttct ggaccagtgc cacgcgctgc tgccggtggt
     6961 ccgtggaggc gggggccggg acgctgctca tcgggtctcc cggttcatcc gcgccccttc
     7021 gcgtgatccg cgctgatcca ccgcaggacg ctgtcctctt cgttggtgcc gatcacctcg
     7081 tgggccagcg ccttgacctc ggggatggcg tttcggaccg ccacggcccg agaggcgatc
     7141 cggaacatgg gcatgtcgtt gacgtcatcg ccgaacacca cgagcggcca gccctggaag
     7201 ccccatcctt cctggagcgc ccggatcccc tggtccttgg tggctttggc gtcgtggacc
     7261 gtgaagaagt gccacccggg aaagtaccgg taggcgtaga tcgtgtagac caggcgattc
     7321 ccgacca
//
LOCUS       DASRZS010000591         7303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2650802, whole genome shotgun sequence.
ACCESSION   DASRZS010000591 DASRZS010000000
VERSION     DASRZS010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2650802"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /locus_tag="VFH83_14370"
     CDS             <1..1503
                     /locus_tag="VFH83_14370"
                     /inference="COORDINATES: protein motif:HMM:NF024686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HET6487608.1"
                     /translation="PSDYNAAVSVSLDTPTAGATIYYTTDGTDPTPATASVYTGAIQV
                     DSTMTLKAMATLKDWADSDVAGGLYTITIVKAVAPPPPPPPQVPAISQSEIDDARGAL
                     ARAKEAGADYYDATNYDAARRLLDDGIALSTKDPATARDRLANATKTANLAFDNSVSR
                     AAADLSARMQAAKQRLLDLEADKWVADDYQRATGLIDDAQSLYASNDLAGARAKAYDA
                     LRAMTNLRNQLATRLTNVRALKNQVEDVMKQAEEAQAGTYAPDQRQNLNNLYLQGLSA
                     FQGYKIDAAEESFGSALQAGRDAVTLARQNRDAQQAAQKQKADELQVQVMKALEDASK
                     LTVVTEDGTVHQPQNWSEDDFLKEIDQMIQQEQPAAPSSGGNQSLVIPSDGGTVVLAD
                     DQQVDLLKQARDLWSQGLKAKADGDYVQAQQYFNEALRYIAVYKSYAVKGVYTVRLIP
                     DRRDCLWRISEYKEIYGDPYLWPVIWRRNRKLIQNPDLIYPGWQLVIPPQ"
     gene            complement(1541..2893)
                     /locus_tag="VFH83_14375"
     CDS             complement(1541..2893)
                     /locus_tag="VFH83_14375"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET6487609.1"
                     /translation="MSIFKSYDIRGIYGTEWDGQTAYRIGRHLPALLGARRMAVGYDA
                     RPSSMEIRRELCRGVLESGCSVADIGMCDTPAVYFATAFYGLDGSVMITASHNPPEYN
                     GLKVSARQAVPVGGDTGLKELEARVAGTLPPMAGGPGAVEPLDIRRDYLSHLGRFRSD
                     LRGLRAVIDCSNGTAGVYLRDVLEATGGSFSYLFDHPDGTFPNHAPNPLIEANLSVIK
                     TRVRGEKADLGICFDGDADRVVFIDEKGRFISPDLVIGLLGMYFFRRHPDRIAGGTNA
                     VTYDVRTSRGVVELLVSLGGDPRICRVGHSFAKKLLRETRGIFGGELAGHYYFRENYF
                     CDSAFMAALAVLELLRLEGRRLSDLVAEVARYFSSGEINFTAANGPEIVARIQADYPN
                     GTLTDIDGIRVDFPDWWFNLRVSNTEPLLRLVVEATSPQGLEERKRELLEKVRRYTRA
                     "
     gene            3093..5630
                     /gene="glgP"
                     /locus_tag="VFH83_14380"
     CDS             3093..5630
                     /gene="glgP"
                     /locus_tag="VFH83_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET6487610.1"
                     /translation="MGLLTFTIKPNIPEKLKPLQELAFNLWISWNYEAIMLFLRLDYD
                     AWQASQQNPVRMLGMVSQERLEQAARDGSFLAALDAVWSKFRRYLEAEPWYPGPRENA
                     IAYFSMEFGLDVSLPTYSGGLGVLAGDHLKTSSDLGLPLVGMGLLYREGYFQQYLNAD
                     GFQQEQYPQNDWYNMPVTLCRDAGGKPMSVTVEMGGSSVEAWIWNVQVGRTSLYLLDT
                     NIDANEPGLRQVTATLYGGDRERRIQQEILLGVGGVRALRALGVRPCCVHMNEGHSAF
                     LGLERIRQLMQENTLTFTEARQAVWATSVFTTHTAVPAGNERFSTALMEKYWGGAVHE
                     MGLEWRDFLALGREDPGNDAEEFSMTVLALRLSAYNNGVSKLHGEVSREMWRRLWPSL
                     PLPEIPIGHITNGVHPRTWVSHDMMELLDRYLGPEFHDSPTTLGNWDRIERVSDEELW
                     RTHERRRERMVAFARTRLTRQLERRGVTNSELTSGASALSPYTLTISFARRFATYKRA
                     DLLLSDPERLETLLTNKDRPVQMIFAGKAHPHDREGKEIIKRLVHFASNPRVSSRIVF
                     LEDYDMTTTRYLVAGSDLWMNTPRRPLEASGTSGMKAALNGALNLSVLDGWWDEAYST
                     GIGWAVGLGEEYDDLGLQDEVEGKAIYDRLEREIIPLFYTRGMDGLPREWIRRMKASM
                     REVGKQFSSHRMLLEYAQRFYQPALANEKAFSANGGALCRETAEYLARLRKSWDVLSV
                     QGLSSPSDSILTVGQRISLKALVGLGDLSPQDVAVELYNGPLSSEGEIEDPRRLEMTY
                     CGMEGKAAVYCAEATCDRTGRLGYTVRVLPKHPALVHPFLPGFVKWG"
     gene            complement(5697..6407)
                     /locus_tag="VFH83_14385"
     CDS             complement(5697..6407)
                     /locus_tag="VFH83_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HET6487611.1"
                     /translation="MIATLVNAAAVLVGALLGTLLRSRIKDSFRSIVQVGAGITSVVI
                     GVQMALSSSRIVYLAVSMILGGILGEWWGIEGGILSLGDTLRRTFARKETGQDFAYGF
                     LNASVLFCVGAMALVGSFRAGAEHNYDLILTKSVMDGFMAIVLTAAMGVGVAFSALSV
                     LVYQGILTIAAGWLKPLVSDQLLKELTGVGGALVIMIGINLLGLARLKTANFIPSLAL
                     IVIAVVVEGVVVSSGILH"
     gene            6523..6966
                     /locus_tag="VFH83_14390"
     CDS             6523..6966
                     /locus_tag="VFH83_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487612.1"
                     /translation="MRRYLLVAVLALVALTAAASDRDPTQLIGMDIRTALQTLGAPQQ
                     MFTVRGVDESEDNVVFFYPDFTYLFWFKDRVWQVRYDRRSAAAVFGITLGMTRDQVHG
                     ANPTPMTSAGDSLYFDLQSAPFPLRVRLVFNAGVLSDLYLYRSDF"
     gene            6963..>7303
                     /locus_tag="VFH83_14395"
     CDS             6963..>7303
                     /locus_tag="VFH83_14395"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET6487613.1"
                     /translation="MIPTRPPGARADICLCLAGPTVQANLDLLARHGSRAEMAELRAD
                     YLDPGEVAAATAMPSRAGVPLILTLRRPAEGGRFPGSERDRIALLERLASGGFALVDL
                     EEDLDAPNLVAR"
BASE COUNT         1310 a         2496 c         2352 g         1145 t
ORIGIN      
        1 ccctcggact acaacgcggc ggtctccgtg agcctcgata cgccgacggc gggtgccacc
       61 atctactaca ccacggacgg gaccgatccg acccccgcga cggcaagcgt ctacaccgga
      121 gccatccagg tggattcgac catgaccctg aaggccatgg cgaccctcaa ggactgggcc
      181 gactcggacg tcgcgggcgg gctgtacacc atcaccatcg tgaaggcagt ggcgccccct
      241 ccgcctcctc ctccccaggt gccggccatc tcccagagcg agatcgacga cgcgcgcggg
      301 gcccttgccc gcgccaagga agccggggcg gactactacg atgccaccaa ctacgatgcc
      361 gctcgccgac tgctggatga cgggatcgcc ctcagcacca aagacccggc cacggcccgg
      421 gaccggctgg caaacgccac caagaccgcc aacctggcgt tcgacaactc cgtatcacgc
      481 gccgcggcgg acctgagcgc ccgcatgcag gccgccaagc agcggcttct ggacctggag
      541 gcggacaagt gggtagcgga cgactatcag cgcgccacgg gcctgatcga tgacgcccag
      601 agcctgtacg ccagcaacga cctggccggc gcccgcgcca aggcatacga cgccctgagg
      661 gccatgacca acctccgaaa ccagctcgcc acccggctca ccaacgtgcg cgccctcaag
      721 aaccaggtgg aggacgtgat gaagcaggcc gaagaggcgc aggcgggcac ctacgctccc
      781 gaccagcggc agaacctgaa caacctctac ctgcaagggc tcagcgcttt ccagggatac
      841 aagatcgacg cggcggaaga gagcttcggc tcggcgctcc aggccgggcg tgacgcggtc
      901 acgctggccc ggcagaaccg ggacgcccag caggcggcgc agaagcagaa ggccgacgag
      961 ctgcaggtcc aggtgatgaa ggcgctggaa gacgcgtcca agctgacggt cgtgacggag
     1021 gacgggacgg tccaccagcc gcagaactgg tccgaagacg acttcctcaa agagatcgac
     1081 cagatgatcc agcaggagca gccggcggct ccgtcttccg ggggcaacca gtccctggtc
     1141 atcccatcgg acggcggcac ggtggtcctg gccgacgacc agcaggtgga cctcctgaag
     1201 caggcccggg atctctggag ccaggggctc aaggccaagg cggacggaga ctacgtccag
     1261 gcgcagcagt atttcaacga ggcgctccgc tacatcgccg tctacaagtc ttacgcggtg
     1321 aagggcgtgt acacggtccg tctgatcccg gatcgccggg actgcctgtg gcggatctcc
     1381 gagtacaagg agatatacgg ggacccgtac ctgtggcccg tcatctggag gcgcaaccgc
     1441 aagcttatcc agaacccgga tctgatctat ccgggctggc agcttgtcat cccgccccag
     1501 taggggaggc cccgcgcggc gctacaggcg caggtaagag ctacgcgcgc gtgtagcgcc
     1561 gcaccttctc gagaagctcc cgctttctct cctcgagtcc ctgcgggctg gtggcctcca
     1621 ctaccaacct caggagaggc tccgtgttcg agactctcag gttgaaccac cagtcgggga
     1681 agtcgacccg gatcccgtcg atgtcggtga gcgttccgtt cggatagtcc gcctggatgc
     1741 gcgccacgat ctcggggccg ttggccgcgg tgaagttgat ctcgccggaa gagaagtagc
     1801 gggccacttc ggcgacgagg tcggagagcc gccgtccctc cagacgcaga agctcgagca
     1861 ccgcgagggc cgccatgaac gccgagtcgc agaagtagtt ctcccgaaag tagtagtgtc
     1921 cggccagctc tccgccgaag atgccccgcg tttcccgcag gagcttcttg gcaaaggagt
     1981 gacccacgcg gcagatcctg ggatccccgc ccaggctcac caggagctcc accacgccgc
     2041 gggacgtcct cacgtcgtac gtcaccgcgt tggtcccgcc ggcgatccga tcgggatggc
     2101 ggcggaagaa gtacattccc agcagcccga tgaccaggtc cggcgagatg aaccgtccct
     2161 tctcgtcgat gaagaccacc cgatccgcgt cgccgtcgaa gcagatcccc aggtccgcct
     2221 tctccccgcg gacccgcgtc ttgatgaccg agaggtttgc ctcgatgagg gggttgggcg
     2281 cgtggttggg gaacgtgcca tcgggatggt cgaagagata cgagaagctt ccccctgtgg
     2341 cttccaggac atcccggagg tagacgcctg ccgttccatt cgagcaatcg atgacggccc
     2401 gcaagccgcg caggtccgaa cggaatctcc ccaggtgcga cagatagtcg cgtcggatgt
     2461 ccagcggctc gacggccccg ggaccgccgg ccatcggagg cagcgtaccg gctacccggg
     2521 cctccagctc cttcaggccc gtgtcacctc ccaccgggac ggcctggcgg gcggagacct
     2581 tcagcccgtt gtactccggc gggttgtggg aggccgtgat catcacgctc ccatcgaggc
     2641 cgtagaacgc ggtcgcaaag tacaccgccg gggtatcaca catcccaatg tccgccaccg
     2701 agcatccgga ttccagcacc cctcgacaga gctctcgccg gatctccatc gatgagggac
     2761 gcgcgtcgta ccccaccgcc atccgccgtg cgcccaggag ggccggcagg tgcctgccga
     2821 tccgatacgc ggtctgcccg tcccactccg tcccgtagat cccgcggatg tcataggatt
     2881 tgaagatgct catcgaggcc tgctccccga gccaccggcg cttcgctccg gcgcggctct
     2941 cctccaaccg tcctgtcgga atgtagcgga tgcgcggcgc gctgtacagc gcggatggtt
     3001 ttcgctacca tgaacaccct cgccggcaga cctctgtcgg acgggcaccg agatacggtt
     3061 cgaagcggaa ccgaagcaca aggaggcggc gcgtgggcct gctcacgttc accataaagc
     3121 ccaacattcc cgagaagctg aaacccctcc aagagctcgc cttcaacctg tggatcagct
     3181 ggaactacga agccatcatg ctctttctcc gcctggacta cgatgcatgg caggcgtccc
     3241 agcagaatcc ggttcggatg ctggggatgg tgtcccagga gaggctggaa caggcggctc
     3301 gggacggatc gtttctggcg gccctcgacg cggtctggag caaattccgc cggtacctgg
     3361 aagcggagcc atggtatccg ggcccgaggg agaacgccat cgcctacttc tccatggagt
     3421 tcgggctcga cgtctccctt ccgacgtatt ccggcggtct gggagtgctg gcaggagatc
     3481 acctgaagac ctcctccgac ctggggcttc ccctggtggg catgggcctg ctctatcggg
     3541 agggctactt ccagcagtac ctgaacgccg acggcttcca gcaggagcag tatccgcaga
     3601 acgactggta caacatgccc gtgacgctgt gccgggacgc cgggggaaag cccatgtccg
     3661 tgacggtcga gatgggcggg tcgtccgtgg aagcgtggat ctggaacgtg caggtcggcc
     3721 gaacctcgct ctacctcctg gacacgaaca tcgacgccaa cgaaccgggg cttcgacagg
     3781 tgaccgccac cctgtacgga ggcgaccgcg agcgtcggat ccagcaggag atcctcctgg
     3841 gggtcggcgg tgtccgtgct cttcgcgcgc tcggcgtccg gccttgctgc gtccacatga
     3901 acgaagggca ctctgccttt ctgggcctcg agcgcatccg acagctcatg caggagaaca
     3961 ccctgacctt cacggaggcc cggcaggcgg tgtgggctac cagcgtcttc accacgcaca
     4021 ccgcggttcc ggcgggcaac gagcggttct ccacggccct catggagaag tactggggcg
     4081 gagcggtcca tgagatggga ctggagtgga gggacttcct ggctctgggg cgcgaagacc
     4141 ccggcaacga cgcggaggag ttctccatga cggtgctggc cctccggctc tccgcctaca
     4201 acaacggggt gagcaagctc cacggcgagg tctcccgcga gatgtggcgt cgcctctggc
     4261 cgtcgctccc gctgccggag atccccatcg ggcacatcac caacggggtc catccccgca
     4321 cctgggtatc ccacgacatg atggagctcc tggaccgcta cctgggaccc gagttccacg
     4381 actcgccgac caccctgggg aactgggacc ggatcgaacg ggtcagcgac gaggagctat
     4441 ggcgcaccca tgaacggagg cgcgagcgta tggtcgcctt cgctcgtacg cgcctcacgc
     4501 ggcagctcga gcgccgcggc gtgaccaact ccgagctgac atcgggcgcg agcgccctgt
     4561 ccccgtacac cctcaccatc agctttgcca ggcgcttcgc cacctacaag cgcgcggacc
     4621 tcctgctctc cgaccccgag cggctggaaa cgctcctcac caacaaggat cgccccgtcc
     4681 agatgatctt tgccggcaag gcgcatccgc acgaccggga aggcaaggag atcatcaagc
     4741 gcctggtgca cttcgccagc aaccctcggg tttccagccg tattgtcttt ctggaagact
     4801 acgacatgac cacgacgcgc tacctggtcg cgggcagcga tctctggatg aacacgccga
     4861 ggcggccgct agaggccagc gggaccagcg ggatgaaggc cgcgctcaac ggagcgctca
     4921 acctctccgt gctggacggc tggtgggatg aggcgtactc cacgggcatc ggctgggcgg
     4981 ttgggctggg ggaggagtac gacgacctgg ggctgcagga cgaggtggag gggaaggcga
     5041 tctacgatcg gctggaacgg gagatcatcc ctctcttcta cacccggggc atggacggcc
     5101 tgccgcggga gtggatccgc cggatgaagg cctccatgcg cgaggtgggc aagcagttca
     5161 gcagtcatcg catgctgctg gaatacgccc agcggttcta ccagccggcc ctggcgaacg
     5221 agaaggcgtt ctccgccaac ggaggcgccc tctgccgtga gacggcggag tatctggcgc
     5281 ggcttcgcaa gtcctgggac gtcctcagcg tccaggggtt gagctctccg tcggactcca
     5341 ttctgacggt cgggcagagg atcagtctga aggccctggt ggggctcgga gatctctcgc
     5401 cccaggacgt ggcggtggag ctttacaacg gtcccctgtc ttccgaaggg gagatcgagg
     5461 atccgcgccg gcttgagatg acctattgcg gtatggaggg caaggcggcg gtctactgcg
     5521 cggaggctac ctgcgatcga accggccggc taggctacac ggtgcgcgta ctgcccaagc
     5581 acccggccct ggtgcacccg ttcctccccg gcttcgtgaa gtggggctga gcgccccggc
     5641 tcggcgtttt tcaacactcc cgcccccggc tgggcgcccc cggctcggcg cccccgtcag
     5701 tgcaggattc ctgagctgac gacgaccccc tccacgacga cggcaatcac gatcagcgcc
     5761 agagacggga tgaagttggc cgtcttcagc ctggccagtc ccagcaggtt gatcccgatc
     5821 atgatcacga gcgctccgcc gacacccgtg agctccttga ggagctggtc gctcacgaga
     5881 ggtttgagcc accccgccgc gatcgtcagg atgccctggt agaccagcac gctgagcgcg
     5941 gagaacgcga cgcccacccc catggccgcg gtgagcacga tggccatgaa cccgtccata
     6001 acggacttcg tgaggatcaa gtcgtagttg tgctcggcgc ccgcgcggaa cgaacccacc
     6061 agcgccatgg cccccacgca gaagagcacc gaggcgttca ggaacccata ggcgaagtcc
     6121 tggccggttt ccttgcgcgc aaaggtccgg cggagcgtat cccccagcga caggattcca
     6181 ccctcgatcc cccaccattc gcccaggatc cctccgagga tcatcgacac cgccaggtac
     6241 acgatccggg atgagctcag ggccatctgc accccgatca ccacggacgt gatgcccgct
     6301 ccgacctgga cgatggaccg aaacgagtcc ttgatgcggg accgcaggag cgtgccgaga
     6361 agcgccccca caaggacggc tgcggcgttg accagggtgg caatcatgtg cccgcactgt
     6421 agcggtttcg gccgggcatg agaagtgccg gcgcccccgc cggccccggc cgggcgcccc
     6481 acttgcaccg agcccgcctg ttaccctact gtgacggctg ccatgaggcg gtacctgctc
     6541 gtggcggtgc tcgcgctcgt cgccctgacc gccgccgcgt ccgaccgcga tcccacgcag
     6601 ctcatcggca tggacatccg cacggcgctt cagaccctgg gcgcgcccca gcagatgttc
     6661 acggtccggg gcgtggacga gtcggaggac aacgtagtct tcttctaccc ggacttcacc
     6721 tacctgttct ggtttaagga ccgggtgtgg caggtgcgat atgaccggag gtccgcagcc
     6781 gccgtcttcg ggatcaccct cgggatgacc cgggaccagg tgcacggcgc caacccgacc
     6841 cccatgacct ccgccgggga ctccctgtac ttcgacctgc agagcgctcc gttcccgctg
     6901 cgggtccggc tggtgttcaa cgccggcgta ctatcggacc tttacctgta ccggagcgac
     6961 ttttgatccc gactcgacct ccgggcgccc gcgctgacat ctgcctctgc cttgcaggcc
     7021 ccacggtcca ggcgaatctg gacctcctgg cccgccacgg ctcccgggca gagatggcgg
     7081 agctgcgcgc ggattacctg gatccggggg aagttgcggc ggccactgcg atgccgtctc
     7141 gcgcaggcgt tcccctcatc ctcacgctcc gccgaccggc cgaggggggc cggttccccg
     7201 gctccgagag ggaccggatc gctctactgg aacggctcgc gtccggcggg tttgccttgg
     7261 tggacctgga ggaagacctg gatgctccga acctggtcgc gcg
//
LOCUS       DASRZS010000592         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2650866, whole genome shotgun sequence.
ACCESSION   DASRZS010000592 DASRZS010000000
VERSION     DASRZS010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2650866"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VFH83_14400"
     CDS             complement(<1..1008)
                     /locus_tag="VFH83_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487614.1"
                     /translation="MGVLHNIRIGIKTQFATVTIVVLFAAVALSVVVLRTVLASAVHR
                     EVVTSNAVNDLNVLNTTLQDYLEGGIGFADAQKSYDAFQSSFKANYSNALTFKFTLDN
                     KKLGASEYLSAVWQEIQKAEDLSKTNAGIEGDVVALTLDSVSKSSDYLSGISARLADP
                     VEQRKVTVLERRVIQGASINTNSAYTIQLRFKDVVKDVTTKDVLFQFLDKAAENSTAD
                     AKSLQGTPFAQLPVDSLASIQKTRALSEQYVANVTQRNAIHDQVKTEMASLASQISEG
                     QARDIQSTFSGILEVMSTALYLFAILVALVVAVQLLVSRSITRPIRHTVDVIKELENG
                     NF"
     gene            1332..1856
                     /locus_tag="VFH83_14405"
     CDS             1332..1856
                     /locus_tag="VFH83_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487615.1"
                     /translation="MKRHSIGRRVLGWVLGVAGTAVTALIVSILVPYLSARLTGAWLP
                     AATEMAQGYLTYPVPAWTILAAVVGILVLRWLVRAIAHRANPDAELFSYTTDTFGPWH
                     FHWNWARDRYGRRTIRNLSPICSVHDTTLKVVGADLVCPRCRRVFPALDRTSLDHFRQ
                     TIAKKAAARYGVEV"
     gene            1888..>2186
                     /locus_tag="VFH83_14410"
     CDS             1888..>2186
                     /locus_tag="VFH83_14410"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6487616.1"
                     /translation="MSGTPERLPTPKLIVFALGQLGWSLASYGVSNLVMYFYVPPETA
                     AAQRIFPPFLFEGVLFGIFTVIGIINAVARFFDAVSNPLLASWSDRSRARMGRRR"
BASE COUNT          366 a          696 c          693 g          431 t
ORIGIN      
        1 gaagttgccg ttctccagct ccttgatgac gtccaccgta tgccggatgg gccgggtgat
       61 cgaccgtgag accaggagct gaaccgcgac caccagcgcc acgaggatcg cgaagaggta
      121 gagcgcggtg ctcatgacct ccaggatccc ggagaacgtc gattggatgt cgcgggcctg
      181 accttcactg atctggctcg ccaggctggc catctctgtc ttgacctggt cgtggatggc
      241 gttgcgctgg gtcacgtttg ccacgtactg ctccgagagg gcccgcgtct tctggatgga
      301 ggccagggaa tccacgggga gctgggcgaa gggggtgccc tggaggctct tggcgtcagc
      361 ggtggagttc tccgcggcct tgtccaggaa ctggaacagc acgtctttcg tcgtcacgtc
      421 cttcaccacg tctttgaacc ggagctggat ggtgtatgcg gagttcgtgt tgatgctggc
      481 cccttggatg accctgcgct ccagcacggt gaccttgcgc tgctccacgg gatccgcgag
      541 tcttgccgaa atgccgctca gatagtcgct ggacttggag accgagtcca gggtgagcgc
      601 caccacgtct ccctcgatgc cagcgttcgt cttggaaagg tcctcggcct tctggatctc
      661 ctgccagacc gcggacagat actccgatgc gcccagcttc ttgttgtcga gggtgaactt
      721 gaacgtcagg gcgttcgagt agttcgcctt gaagctgctc tggaaggcgt cgtagctctt
      781 ctgggcatcc gcgaagccga tgcctccttc caggtagtcc tggagagtcg tattcaggac
      841 gttcagatcg ttcacggcat tcgacgtaac cacctcgcga tgcacggcgg acgccagaac
      901 cgtacgaagg accacgaccg acaacgccac cgcggcgaac aggactacga tcgtcacggt
      961 cgcgaactgt gtcttgatgc cgattcggat gttgtgaagc acgcccatat gcggcctcct
     1021 tgccgacggt atgattgcaa gcaggatgcc aaggcggcgt gacaggaccc acgtccttgg
     1081 attgcgcttc ccaactggtt gccgaataaa gagataacgc gccctggcag gaccggcccc
     1141 gacgccgccc gggccggagc ccgcgcgcac cgccggaccg aatccgcaac ttttctttat
     1201 gatgaaacgt ttgttgaggt cgacgaggcc tgggctcgac atcgggggtg atgggcgggg
     1261 cactcgacgc agcacctcat tccggctacc gtcaaccgaa tggaggcggc gaccgctcca
     1321 gaaaatccga gatgaaacga cactcgatcg gtagaagagt tctgggctgg gtgctcggcg
     1381 tggcggggac cgccgtaacc gccttgatcg tttccatcct cgtgccgtat ctcagcgcgc
     1441 gtctcacggg ggcatggctc ccggcggcga cggagatggc tcaggggtac ctgacctacc
     1501 ccgtaccggc ctggaccatc ctggccgcgg tggtcggcat cctggtgctc cgctggctgg
     1561 ttcgggcaat cgctcaccgg gcgaacccgg atgccgagct cttttcttac accacggaca
     1621 ccttcggccc gtggcacttc cactggaact gggcccggga ccgttacggg cggcggacca
     1681 tccgcaacct cagccccatc tgctccgtgc acgacacgac gctgaaggtg gtcggcgctg
     1741 acctggtctg ccccaggtgt cgtcgggtct tcccggccct ggatcgcacc tctctcgacc
     1801 acttccgaca gaccattgcc aagaaggccg cggcccgata cggggtagag gtctagaaca
     1861 gccggccgcc cgcgcgggag gaacagcatg agtgggacac cggagcgcct gcctacgccg
     1921 aagctcatcg tcttcgccct gggtcagctc ggctggtctc tcgcttcgta cggggtaagc
     1981 aacctcgtga tgtatttcta cgtcccgccg gagaccgcgg ccgcccagcg gatctttccg
     2041 ccgttcctgt tcgagggcgt cctcttcggg atcttcacgg tcatcgggat catcaacgcc
     2101 gtggcccggt tcttcgacgc ggtgagcaat ccgctcctgg cctcgtggtc cgatcgatcc
     2161 cgcgcccgga tggggcgtcg ccgctt
//
LOCUS       DASRZS010000593         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1116136, whole genome shotgun sequence.
ACCESSION   DASRZS010000593 DASRZS010000000
VERSION     DASRZS010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116136"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1178
                     /locus_tag="VFH83_14415"
     CDS             129..1178
                     /locus_tag="VFH83_14415"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6487617.1"
                     /translation="MPTSRFSFAVVGLGLGAEHARRILSRPLEWELAAVCEPLGDRRA
                     AFTRAHPEVRAFASLDALLEASTPDGVVLAVPHHLHAPMAVQALRAGAHVLVEKPMAR
                     TTEECLRMNEAARQTGRTLMVAQNWRYTPWVRVAKAILDRGDLGAIRVVRTEWLQNIT
                     ADTSRPGNWLLDGERAGGGPVISLVVHNLDALRYLLGEPVEVHAFCLHDEALFRNGAE
                     SWGIAQFRFQNGAVGQMMSSYAACPALDEGGRLSIHGERGTLSANGGLPAANLLRVYT
                     AATGAWETPRLDSVPGLPTSDPMLNEISHFVDCARSGAPSLSGGEDNIRTIRFVQTIY
                     ESSRRGVALPITAGP"
     gene            complement(1191..2000)
                     /locus_tag="VFH83_14420"
     CDS             complement(1191..2000)
                     /locus_tag="VFH83_14420"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6487618.1"
                     /translation="MSRRRVWAIVRKDLTEVTSSKSVMVPMVIAPLVLCVVLPAVVLL
                     VGLSGATAKLNGMDLIGKLVARYPVPAGFGSIMDQVMFVFLNYSFLPFFMIIPVLASS
                     IIAANSVVGEKERGTLETLLYTPISNRELVEAKLLSSFIPSLVVTAGGFLGYFLVSNA
                     IFWAYRGQLIVRSVIWIPALLLLAPSVSLLGLSITLLVSLRAKTYIEAQQLSAMVVIP
                     FVVLLYAQIGGLLVLSPLWVVGLGIAALVVSYVIYGKIGPRFSREQILMTL"
BASE COUNT          368 a          672 c          674 g          305 t
ORIGIN      
        1 ccgctgccgc ggggaactgg cggtggatct gtgccaggtc gagtgccacc gccttgaatg
       61 tactatcgtg gagagcccag gaggagccat tgccgggagc acggcaatgg gccagaatcg
      121 aggtcatcat gccaacgtca cggttctctt tcgcggttgt cggcctcggc ctgggcgcgg
      181 agcatgcccg gcgcatcctt tcccgaccgc tggagtggga gctcgccgcg gtctgcgagc
      241 cgctggggga tcggcgcgcg gccttcacgc gggcccatcc ggaggtccgc gcattcgcat
      301 cgctcgacgc gctgctggag gcatcgactc cggacggcgt cgtgctggcc gtgccgcacc
      361 acctgcacgc tccgatggcg gtccaggccc tgcgcgcggg tgcccacgtg ctcgtggaga
      421 agccgatggc gcgcaccacc gaggagtgcc tccggatgaa cgaagcggcc cggcaaaccg
      481 gcaggacgct catggtggca cagaactggc gctacacccc gtgggtgcgc gtcgccaagg
      541 ccatcctgga ccgaggcgac ctgggggcaa tccgggtggt ccgcaccgag tggcttcaga
      601 acatcacggc ggatacgagc cggcccggca actggcttct ggacggagag cgcgccggcg
      661 ggggcccggt gatcagcctc gtggtgcaca acctcgacgc cctgcggtac ctgctgggcg
      721 agcccgtgga agtgcacgcg ttctgcctcc acgacgaagc gttgttccgt aacggcgccg
      781 aaagctgggg catcgcccag ttccgattcc agaacggcgc cgtgggacag atgatgagca
      841 gctacgccgc ctgtcccgcg ttggatgagg gtggcaggct gtccatccac ggagagcggg
      901 gaacgctctc ggcgaacggc gggctgcccg ccgccaacct gcttcgggtc tacaccgccg
      961 ccacgggcgc gtgggagacg ccgcggctgg actccgtccc cggtcttccg acctcggatc
     1021 ccatgctcaa cgagatctcc cacttcgtgg actgcgcccg cagcggggcc ccgtcgctga
     1081 gcggcggaga ggacaacatc aggacgatcc gcttcgtgca gaccatctac gagtccagcc
     1141 gtcggggcgt ggccttgccg atcacggccg gaccgtagcc gcgccggtcc ctacagcgtc
     1201 atcaggatct gctcccggct gaatcgcgga ccgatcttcc cgtagatcac gtagctcacg
     1261 accagcgccg cgatgcccag gccaaccacc cagagagggc tcagcaccag gagcccgccg
     1321 atctgcgcgt acagcagcac cacgaagggg atcaccacca tcgcggagag ctgctgcgct
     1381 tcgatgtacg tctttgccct cagggatacc aggagcgtga ttgagagccc caggagagag
     1441 accgagggcg ccagcaggag cagggcgggg atccagatga cggagcgaac gatgagctgg
     1501 ccgcggtacg cccagaagat cgcgttggag accaggaagt accccaggaa accgccggcg
     1561 gtcaccacga gggagggtat gaaggagctc aggagcttgg cctccaccag ctcgcgattg
     1621 ctgatgggcg tgtagagcaa cgtctccagg gtgcctcgct ccttctcccc cacgacggag
     1681 ttggcggcga tgatggagga ggcaaggacc gggatgatca tgaagaacgg caggaagctg
     1741 tagttgagga acacgaacat cacctggtcc atgatggagc cgaaccccgc cggaacgggg
     1801 tatcttgcca ccagcttccc gatcagatcc atgccgttca gctttgccgt agctccactc
     1861 agcccgacga gaaggaccac ggccggcagc accacacaga gcaccagcgg cgcgatcacc
     1921 atcggcacca tcacggactt gctcgacgtg acttccgtca gatccttacg tacgatcgcc
     1981 cacaccctac gccggctcat ggcttctcct cccgatgtg
//
LOCUS       DASRZS010000594         4066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5716337, whole genome shotgun sequence.
ACCESSION   DASRZS010000594 DASRZS010000000
VERSION     DASRZS010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5716337"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VFH83_14425"
     CDS             <1..659
                     /locus_tag="VFH83_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487619.1"
                     /translation="LVTHSHWDHLMDVPEIVSYTGATVIGSSNTAQLLTLLGVAPANI
                     SVTRAGQSFRVRDFRVRVFEATHLRAPGFGPGKLPANLRPPLRARDYRMDRLFRFHVE
                     VDGISTATEPGDGPWQAQDTSILLMSPRHAEDRLAQILQRTHPTTVIPYHWDDMYRPL
                     WKPLRPMMAPPTFSLRPFRRIDPRGFQGRVRRIDPRVRVFYPEVLQTYCLEDLVATGK
                     "
     gene            931..1677
                     /locus_tag="VFH83_14430"
     CDS             931..1677
                     /locus_tag="VFH83_14430"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET6487620.1"
                     /translation="MLERTLSRFTLDMRQLLGQNLLEVIIHGSVVLGDFRPGRGDIDY
                     VVITKDDLDEEVNQEIFRIHDRYRGSKELLLYQLEGPFYPRRAVMDLGSAFMGCYIGT
                     TRRYWRAITSFQNSLMDLAIIDRYGRRMLGTATPLYRPSSEEIHAEQVKDCGKLAASM
                     QGREAPSMADFVSTIHWCARTIHYLRGGQIVSKGTACRWCHESPELDSFGELFEYAQE
                     RRYPYEPRSADPAMVGACRALLDKVGRLLG"
     gene            1674..2552
                     /locus_tag="VFH83_14435"
     CDS             1674..2552
                     /locus_tag="VFH83_14435"
                     /inference="COORDINATES: protein motif:HMM:NF016334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HET6487621.1"
                     /translation="MMRSDDDMFALILRFAREQSAVRAAVLNGSRANPNVRRDPFQDF
                     DIVYLTNDLGRFTRQDWIPRYFGDIMILQLPDDMVDPPPDGIHGYTYLMQFVDGTRID
                     LGFQPLAAARSVVSDSLSVVLLDKDRLLDGTPPASDVDYLPSPPTRKLFDDCCNEFWW
                     LNPYVAKGLWRDELPYAKHAMETLVRPQVMKMVTWDAAARTGYRTALGKWGKHLKDHL
                     DPADWRSLVATYADGKPENLWAALHEAQKLFRRVALHVAQANGYEYPEREDRRVSDFV
                     ARIEALPRDVRDILDP"
     gene            complement(2574..2942)
                     /locus_tag="VFH83_14440"
     CDS             complement(2574..2942)
                     /locus_tag="VFH83_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487622.1"
                     /translation="MAASKKTPVPEEKLRLYERLVATNANVTRKGDSVPYTSVNGHMF
                     SYLNSAGSMALKLPKEEIEGFLKRYHTTLFHAYGIIQKEFVTVPDSLLGKTTELKKYF
                     DLSYDYVSKLKPKPTKKPAR"
     gene            3049..3840
                     /locus_tag="VFH83_14445"
     CDS             3049..3840
                     /locus_tag="VFH83_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6487623.1"
                     /translation="MDVRDLNRTAWDRLVDRGIRWSIPVGAESITAARNGEWEIYLTP
                     TRPIPKAWLPTVAGKQILCLASGGGQQGPILAAAGAVVTVLDNSPRQLHQDRLVAERE
                     GLRIETVEGTMKDLSPFAANRFDYIVHPVSNCFVDDVRPVWNEAYRVLRPGGALLSGF
                     NNPDIYLFDVEVMASAGKLEARNRLPYSDLERLDAEQRSSKAEQGVPMEFSHTLELQI
                     GGQMEAGFTLIGLYEDRDLPGENALINQYLRPYIATRAVKHSVSP"
     gene            3852..>4066
                     /locus_tag="VFH83_14450"
     CDS             3852..>4066
                     /locus_tag="VFH83_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487624.1"
                     /translation="MIELVLDDNGAIEIGDCSPDSTLQELVDAVGAWITGHGACRGCT
                     RCCRDVIPLNGIEVRSLKVSPQDVSMA"
BASE COUNT          759 a         1278 c         1308 g          721 t
ORIGIN      
        1 tgctggtgac ccactcgcac tgggatcacc tgatggatgt gccggagatc gtcagctaca
       61 ccggtgcgac ggtgatcggc tcgtccaaca cggctcaact gttgacgttg ctgggggtgg
      121 cgcctgcgaa tatctccgta acccgggccg gccaaagctt cagggtccgt gacttccgcg
      181 ttcgggtgtt cgaggccacc cacctgaggg cgcccggatt cggaccaggg aagctaccgg
      241 ctaacctccg gcctcccctg cgcgccagag actaccgcat ggaccggctc ttccgttttc
      301 acgtcgaggt ggatggcatc tcaacggcca ccgagcccgg agatggcccc tggcaggcgc
      361 aggacacatc catcttgctg atgagcccgc gtcacgcgga ggaccggctg gcacagatcc
      421 tccagcggac ccatcccacg acggtgattc cttaccactg ggacgacatg tatcgccccc
      481 tgtggaagcc cctaaggccg atgatggcgc cgcccacctt ctccctcagg ccgtttcggc
      541 gaatagaccc gcgcggcttc caagggaggg ttcgacggat cgatccgcgg gttcgagtgt
      601 tctatcccga ggttctgcaa acctattgcc tcgaggacct cgtggcgacc gggaagtgac
      661 gacgccacac ccggggagca acgacgctaa tcccgcgaga cggcgcccag gagaacggcc
      721 agctcctgaa cgccgccgaa gcggggtcac gccaaccgca tgcgtggccg agctgacccg
      781 tcgcctcctg gccgatggac actccttttg cacgccgtac gcggacgtcg ccaacaagcg
      841 agtcgaactc catctactcc agactgggct acgagccatg gggcaggttg tcgtgctcga
      901 gctcggctac ccgctcgtgg gaggcaggtc atgctggaac gaaccttgtc tcgattcacc
      961 ttggacatgc ggcagctcct cggccagaac ctcctcgagg tgatcatcca cggatcggtc
     1021 gtgctgggag atttcaggcc gggccgaggg gatatcgact acgtggtcat caccaaggac
     1081 gacctggatg aagaggtcaa ccaggagatc ttccggatcc acgaccggta tcggggatcc
     1141 aaggagctgc tgttgtacca gctcgagggg cccttctatc cgcggcgcgc cgtgatggac
     1201 ctggggagcg ccttcatggg ctgctacatc gggaccaccc gaaggtactg gcgagccatc
     1261 acatcgttcc agaacagcct gatggatttg gcgatcatag accgatacgg ccgccgaatg
     1321 ctgggcaccg ccaccccgct ttatcgaccc agctcggagg agatccatgc ggagcaggtg
     1381 aaggactgcg gaaagctggc cgcctcgatg caggggcggg aggcgccatc catggcggac
     1441 ttcgtttcca cgatccactg gtgcgccagg accatccact acctccgcgg cggccagatc
     1501 gtctccaaag ggaccgcctg tcgctggtgt cacgagtccc cggaactgga ctcgtttggg
     1561 gagctgttcg agtacgctca ggaacggcga tacccgtatg agccccgatc cgcggacccc
     1621 gccatggttg gcgcatgcag ggcgctgctg gacaaggtcg ggaggctgct cggatgatgc
     1681 gtagcgacga tgacatgttc gccctcatct tgaggttcgc tcgggagcag agcgccgtcc
     1741 gagccgccgt cctgaatggg tcccgagcca accccaacgt ccggcgcgac ccattccagg
     1801 acttcgacat cgtgtatctc accaacgatc tggggaggtt tacccgccag gactggatac
     1861 cacgctactt cggcgacatc atgatcctgc agctccccga cgacatggtc gatccgccgc
     1921 ccgacggaat ccacggatac acttatctca tgcagttcgt cgacgggacc cggatcgacc
     1981 tcggctttca gccgctcgcg gcggcgcggt ccgtcgtcag cgactccttg tcggtggtcc
     2041 ttctcgataa ggaccgcctc ctggacggga ccccgccggc cagcgacgtc gattatctgc
     2101 cgagcccacc gacgcgcaag ctcttcgatg actgctgcaa cgagttctgg tggctcaacc
     2161 cgtacgtggc gaagggactg tggagagacg agctgccgta cgcgaagcac gcgatggaga
     2221 cgctggtcag gccccaggtc atgaagatgg tcacctggga cgccgcggct cggacgggct
     2281 accggacggc cctgggcaag tggggcaaac acctgaagga tcatctggac cccgccgact
     2341 ggcgcagcct ggttgcgacc tacgcggatg ggaagccgga gaacctgtgg gccgcgctgc
     2401 acgaggcgca aaagctgttc cgcagagtgg cgctccacgt ggcccaggca aacgggtacg
     2461 aatacccgga gcgtgaggac cgcagggttt cggacttcgt ggcacggatc gaagcgttgc
     2521 cgcgagacgt gcgcgacatc ctcgacccat gagcgcggcg ccggcgcccg gtgctaccgg
     2581 gccggcttct tcgtaggctt tggcttgagc ttgctcacat agtcatagct caggtcgaag
     2641 tacttcttca gctcggtcgt cttcccgagg agggaatcag gaacggtcac gaactccttc
     2701 tggatgattc cataggcatg gaacagcgtg gtatggtacc tcttcaggaa gccttcgatc
     2761 tcttccttgg gaagcttgag cgccatcgat ccggcggagt tcaggtagct gaacatgtgc
     2821 ccattcaccg aggtgtacgg cacggagtcg cccttgcggg tgacgttcgc attggtggcg
     2881 accagtcttt cgtacaaccg cagcttttcc tcagggacag gcgtcttctt ggaagcggcc
     2941 atcggcatcc tccgattgac ggactccggc tctctcggca ccgttgaagc taccagggac
     3001 ggcacctggt gggcaaggca gtgagaggcg gtgaagggag cgattcgcgt ggatgtgaga
     3061 gacctgaacc gaaccgcgtg ggacaggctg gtcgacaggg gcatccgatg gtccatcccg
     3121 gtcggagcgg agagcataac cgccgcccga aatggggagt gggagatcta cctcaccccc
     3181 acacggccca tcccgaaagc ctggctgccc acggtagctg ggaagcagat cctttgcctg
     3241 gcctccggag gaggacagca gggacctatc cttgcggccg ccggcgcggt ggtgaccgtg
     3301 ctcgacaatt cgccccgcca gctccatcag gaccggctgg tagccgagcg agagggtctg
     3361 aggatcgaga ccgtggaggg aaccatgaag gatctctccc cgttcgcggc gaatcggttc
     3421 gactacatcg tgcatccggt ctcgaactgc ttcgtggacg acgtgcgccc ggtgtggaac
     3481 gaggcgtacc gggtcctgag acccggcggg gccctgctct cgggtttcaa caacccggac
     3541 atctacctgt tcgacgtgga ggtcatggcc tccgcgggga agctggaggc gaggaaccgc
     3601 ctgccgtact cggacctgga gcggctggac gcggagcaga gaagctccaa ggccgagcaa
     3661 ggtgttccca tggagttcag ccacacgctg gagctgcaga tcggcgggca gatggaggcg
     3721 gggttcacgc tcatcggcct ctatgaggat cgagacctgc cgggggagaa tgctctcatc
     3781 aaccagtatc tgcggccgta catcgccacg agagccgtca agcactccgt ctccccctga
     3841 agtcgttgcc gatgatcgag cttgtcctcg acgacaacgg agccattgag atcggagact
     3901 gctccccgga cagcacgctc caggagctgg tcgacgccgt gggcgcatgg atcaccggtc
     3961 acggagcctg tcgtggctgc actcggtgct gcagggacgt catccctctg aacggtatcg
     4021 aggtcaggag cctcaaggta tctccccaag atgtctcgat ggccga
//
LOCUS       DASRZS010000595         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3034685, whole genome shotgun sequence.
ACCESSION   DASRZS010000595 DASRZS010000000
VERSION     DASRZS010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3034685"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFH83_14455"
     CDS             complement(<1..486)
                     /locus_tag="VFH83_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6487625.1"
                     /translation="MVEIRDGSQLEEALSEPPDALVTALSELDGDILVLGAGGKMGPT
                     LTRMARRAADAAGSKRRVIAVSRFTDPTVRSRLEAAGVSTISCDLLDEAALGKLPDAP
                     NVVYMAGMKFGTTGNEPLTWAQNAYLPGLALRRYRGNRLVVFSSGNVYGLTPVARGGS
                     VE"
     gene            557..1864
                     /locus_tag="VFH83_14460"
     CDS             557..1864
                     /locus_tag="VFH83_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009179887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HET6487626.1"
                     /translation="MYWNRDLETMDRLTLERLQLERLNATLGRAVRAPFYQGRFGANG
                     GPRLASLDELCRLPFTTKADLRDGFPYGFLAVGLDRIVRLHSSSGTTGNPTVIYHTKE
                     DIEAWQDLVARCMYMAGMRKTDVFQNMMGYGLFTGGLGFHYGAEKLGALTIPIGPGTS
                     KRQIWFMQQFRTTAVHVLPSYALRLATHIAEMGLDPAKDFCLRMAFVGAEPHTEETRR
                     KIEQTYGIKVYNSYGLSEMCGPGVAFECPEQSGMHIWEDQFLVEIIDPETRRPLPEGE
                     CGELVLTTINREGTPLLRYRTRDLTRIIPGPCPCGRVHRRIDRIRGRSDDMMIINGVN
                     VFPMQIEQTLMRIPGIGTNYLIELQEDNFMDKLHVHVELAPDAFQGTLSGIESLQNRV
                     LAALREETGVTPVVKLLEAGGLPAPEGKAVRVVDRRKQSGKGC"
     gene            1866..2294
                     /locus_tag="VFH83_14465"
     CDS             1866..2294
                     /locus_tag="VFH83_14465"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET6487627.1"
                     /translation="MAHHLSVFVTNEPGKLERITRSLGEHGLNIRAISMASAGAFGVV
                     RVLVNDPDRGLAALQEARFTVAKRRILVALVDDRPGALHDLLVTLSANQVNVEDCYGF
                     VLEEGRKAAIVFEVERFPEAEKVLASTSIELISDAEIYRL"
     gene            2307..>2973
                     /locus_tag="VFH83_14470"
     CDS             2307..>2973
                     /locus_tag="VFH83_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HET6487628.1"
                     /translation="MIWNNDYESLDRADLSRLQGERLGRLVDVLYRRVPFYRKKMEAA
                     GVRPNDIQGIQDIARLPFTTKDEMRDVYPYGLLAVDLREIVEIHTSSGTTGKPVVDAY
                     TSADLDVWSEAMARTLAAGGTTRHDIVQNAYGYGLFTGGLGVHYGARTIGATVIPISG
                     GNTKRQLAVLQDFKATMLTCTPSYSLYLAEAAREEGLDLSKIGLKSGFFGAEPWSESM
                     RKEI"
BASE COUNT          528 a          989 c          988 g          468 t
ORIGIN      
        1 ctccacggag ccgccgcgag ccaccggggt gagcccgtag acgttgccgg aggagaacac
       61 gaccaaccgg tttccccggt accgccgaag ggccagcccg ggaagatagg cgttctgcgc
      121 ccaggtgagc ggctcgttgc cggtggtccc gaacttcatt ccggccatgt agactacgtt
      181 gggcgcgtcc ggaagcttcc cgagggcggc ctcgtccagg agatcgcagg agatggtgga
      241 gaccccggcc gcctccagac gggagcggac ggtcgggtcg gtgaaccggg agaccgcgat
      301 cacccgacgc ttgctccccg cggcgtcggc cgctcgacgc gccatccggg tcagagtcgg
      361 tcccatcttt ccgccggcgc ccaggaccag aatgtccccg tccagctccg acagcgccgt
      421 aaccagggcg tccgggggct cgctcagcgc ttcctcgagc tgactcccgt cacgaatctc
      481 taccatggac ccgagtctac tccggcagcg tcgccttgca aaagtatgca cacttggtat
      541 aacagagacc aagaccatgt attggaaccg ggatctcgag accatggacc gtctcacgct
      601 cgagcggctg cagctcgaac gcctgaatgc caccctgggc cgcgcggtgc gggccccgtt
      661 ctaccagggg cgcttcggcg cgaacggcgg gccgaggctc gcctccctgg atgagctttg
      721 ccgcctgccc ttcaccacca aggcggacct tcgggatgga ttcccgtacg gcttcctggc
      781 cgtggggctg gatcggatcg ttcgtctgca ctcttcttcc ggaaccacgg gcaaccccac
      841 ggtgatctac catacgaagg aggacatcga ggcgtggcag gacctggtgg cgcgctgcat
      901 gtacatggcg ggcatgcgca agacggacgt gttccagaac atgatgggct acggcctgtt
      961 caccgggggg ctcgggttcc actacggcgc ggagaagctg ggggccctca ccattcccat
     1021 cgggcccggg accagcaagc gccagatctg gttcatgcag cagttccgga ccaccgcggt
     1081 ccacgtcctt cccagctacg cgctgcgcct ggccacgcac atcgccgaga tggggttgga
     1141 tccggccaag gacttctgcc ttcggatggc cttcgtggga gcggagcccc acacggagga
     1201 gacccgccgc aagatcgagc agacctacgg catcaaggtc tacaactcct atggcctctc
     1261 ggaaatgtgc ggccccggtg tggcgttcga gtgtcccgag cagtccggca tgcacatctg
     1321 ggaagaccag ttcctggtgg agatcatcga tcccgagacg cgccggcccc tgccggaggg
     1381 ggaatgcggc gagttggtcc tgaccacgat caaccgggaa gggacgcccc tcctgcgcta
     1441 tcgcacccgg gacctgaccc gcatcatccc cgggccgtgc ccgtgcggaa gggtgcatcg
     1501 acggatcgac cggatccgcg gccgctcgga tgacatgatg atcatcaacg gcgtgaatgt
     1561 attccccatg cagatcgagc agaccctcat gcggatcccg ggcatcggga ccaactacct
     1621 gatcgagctg caggaagaca acttcatgga caagcttcac gtgcacgtcg agcttgcgcc
     1681 ggacgcgttc cagggcaccc tctccggaat cgagagcctg cagaaccggg tgctggcggc
     1741 cctccgggag gagaccggcg tcacgcccgt ggtgaagctg ctggaggcag gagggctgcc
     1801 ggccccggag ggcaaggcgg tccgcgtggt ggaccggcgc aagcaatccg gaaaggggtg
     1861 ctagcatggc gcatcacctt tcggtcttcg tcaccaacga gccgggcaag ctggagcgca
     1921 tcacccggag cctcggcgag cacggtctca acatccgcgc catctccatg gccagcgccg
     1981 gagcgttcgg agtggtccgg gtgctggtga acgacccgga ccgcggcctt gcggctctcc
     2041 aggaggcccg cttcacggtg gccaagcggc ggatcctggt ggccctggtg gacgatcggc
     2101 cgggcgccct gcacgatctc ctggtcacgc tctccgccaa ccaggtgaac gtggaggact
     2161 gctacggctt tgtcctggaa gagggccgca aggccgccat cgtgttcgag gtggaacgat
     2221 tcccggaggc cgagaaggtg ctggcctcca catccatcga gctgatctcc gacgcggaga
     2281 tctatcgact gtaacgggag cggaccatga tctggaacaa cgactacgag agcctggacc
     2341 gcgcggatct gtcgcggctc cagggcgaga ggctgggacg gctggtggac gtgctctacc
     2401 gacgggtgcc gttctaccgc aagaagatgg aggcggccgg cgtccggccc aacgacatcc
     2461 aggggatcca ggacatcgcc cggctcccct tcaccaccaa ggacgagatg cgggacgtct
     2521 atccgtatgg actgctggcg gtggatctgc gggagatcgt ggagatccac acctcgtccg
     2581 gcaccacggg caagccggtg gtggacgcct acaccagcgc cgacctggac gtctggtcgg
     2641 aggccatggc gcggacgctc gcggccggcg gcaccacgcg ccatgacatc gtccagaacg
     2701 cgtacggata cggcctgttc accggaggcc ttggcgtcca ctacggcgcc cgaaccatcg
     2761 gcgcgaccgt catcccgatc tccggcggca acaccaagcg ccagctcgcc gtcctccagg
     2821 acttcaaggc caccatgctg acctgcaccc cctcctactc gctgtacctg gccgaggcgg
     2881 cgcgggagga gggtctggac ctctccaaga tcggtctgaa gtccgggttc ttcggcgccg
     2941 agccgtggag cgagagcatg cgcaaggaga tcg
//
LOCUS       DASRZS010000596         3157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3802470, whole genome shotgun sequence.
ACCESSION   DASRZS010000596 DASRZS010000000
VERSION     DASRZS010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3802470"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /gene="fliD"
                     /locus_tag="VFH83_14475"
     CDS             <1..830
                     /gene="fliD"
                     /locus_tag="VFH83_14475"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HET6487629.1"
                     /translation="SLTMDGVQVHRPSNTVDDLLPGVTLTLKTPAATPTTITVKHDVE
                     GIKRQIVSLVGAYNQIITNVDVLTRKDQSIIDNTQWDSDDAKKKAQESLGLLTGEITL
                     QQIKSSLQLTMMGPYPTSLDRDMRLLAQIGISTDARPPGSGGIDKTRLRGYLEIDDAK
                     LTAALQDHADAVKQLFGNDTDGDLVVDSGVAFKLDALLRPYVQTGGILALRVSGLDSR
                     IAQTKKDITDLKAALDDKQAELKQKYAQMAGALDDLNKNSQDITNFNRQQGSGQSGQ"
     gene            842..1702
                     /locus_tag="VFH83_14480"
     CDS             842..1702
                     /locus_tag="VFH83_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487630.1"
                     /translation="MQLTINGERVDYSLESEKTLAEVARGVRAWLDQAGFMISGFRAD
                     GKDLLSAPAEQWAALPVSEVASLEVEAAHTGSLRIDHWRTVDTWLELLERELSSSSGS
                     LAELLGAVNESLDGIRANPFLPTGSPDVSRFDLLFRGQTPQQVGSWPPDRVAEARAVL
                     SRLRASLAARLADAEQPEQALGRCVARLRDQLPRLPEVSVLLQTGRDRDAMALVIGFT
                     DTANSIVELLPFLPPDPERGKLLVELTPFLRELAGAFDTKDSVLIGDLLEYEITPRMQ
                     RLLPMLERAS"
     gene            1699..2865
                     /locus_tag="VFH83_14485"
     CDS             1699..2865
                     /locus_tag="VFH83_14485"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6487631.1"
                     /translation="MILLQTGVQNVYTSSESSTAIYFGLAVAGVAIVLLVFAFINRRV
                     RARNPEETQQYSSYQFRRLAKSYGLSSQHIEMLERLVTVCKVRQPMLIFSSVGLLDDT
                     LKKGLYSLEGVRDATPEERENQKALIFEIKQVLEQGGRRVGAVSSTTALKPGQELTIR
                     AGTSQFASRVVSNMRDFLTVAAPPVTTDAQARWVRGTQLVVHLWRDGDAGYAFESKIL
                     GYDTVKGIPSILIQHSKTLRKEQRRKNRRRELLRPCFFYPIRVAEVGQGRQAAVKAVV
                     EQDKRALGHVVDISAGGCSIQTLVPLEKGKLLMVEFEIGRGNTIRTYGKAVGLYRRPR
                     RGGIMHVMFTRVTRQYLNRISEFVYDFARPSTVGEARLQLDRRPAMGGTVSRAR"
     gene            3010..>3157
                     /locus_tag="VFH83_14490"
     CDS             3010..>3157
                     /locus_tag="VFH83_14490"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007391405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET6487632.1"
                     /translation="MGEELAVLSDGSVLILIGPLGAGKTVLAKGIALGLGVQEPVVSP
                     TYTIV"
BASE COUNT          555 a         1081 c         1045 g          476 t
ORIGIN      
        1 cgtccctcac catggacggc gtgcaggtcc accggccgtc caacacggtg gacgacctgc
       61 tccccggcgt caccctcacg ctgaagactc ccgccgccac gcccacaacg atcacggtga
      121 agcacgacgt ggaggggatc aagcggcaga tcgtgtccct ggtaggcgcc tacaaccaga
      181 tcatcacgaa cgtcgacgtc ctgacccgca aggaccagag catcatcgac aatacccagt
      241 gggattcgga cgacgccaag aagaaggcac aggagagcct cgggcttctg acgggggaga
      301 tcaccctgca gcagataaag agctcgctgc agcttacgat gatgggcccc taccccacgt
      361 ccctggaccg ggacatgcgc ctgctggccc agatcggcat ctccacggac gcccggcctc
      421 ccggttccgg cggcatcgac aagacccgtc tgcggggata tctggagatc gatgacgcca
      481 agctcacggc cgccctccag gatcacgcgg acgccgtgaa gcagctcttc gggaacgaca
      541 cggatggcga cctggtggtc gattccggcg tggccttcaa gctggacgcc ctcctgaggc
      601 cttacgtgca gaccggcggg atcctggccc ttcgggtctc cggtctcgat tcccgcatcg
      661 cccagaccaa gaaggacatc accgacctga aggcggcgct ggacgacaag caggccgagc
      721 tgaagcagaa gtacgcccag atggccggag cgctggacga cctcaacaag aactcccagg
      781 acatcacgaa cttcaaccgc cagcagggct ccggccagag cgggcagtag agagaggcat
      841 cgtgcagctg accatcaacg gagagcgagt cgactactcc ctggaatccg agaagaccct
      901 ggcggaggtg gcgcgcgggg tccgcgcctg gcttgaccag gccgggttca tgatcagcgg
      961 gttccgcgcg gacggaaagg acctcctgtc cgcgcccgcg gagcagtggg cggcgcttcc
     1021 cgtctccgag gtcgcctccc tggaagtcga agccgcgcac acgggaagcc tgcgcatcga
     1081 ccactggcgc acggtggaca cgtggctgga gctgctggag cgcgagctct cgtcgagctc
     1141 gggatccctg gcggagcttc tcggcgccgt caacgagagc ctcgacggga tacgggccaa
     1201 cccctttctg ccgacgggct ctccggatgt ctcgcggttc gacctgctct tccgggggca
     1261 gacaccgcag caggtcggct cctggccgcc ggatcgggtg gcggaggcgc gcgccgtcct
     1321 ctcccggctg cgggcgtcgc tggcggcccg gctggcggat gccgagcagc cggagcaagc
     1381 cctggggcgg tgcgtggcgc ggctccggga ccagctcccc agactcccgg aggtctcggt
     1441 ccttctccag acgggccgag accgggacgc catggcgctg gtgatcgggt tcacggatac
     1501 cgcgaactcg atcgtcgagc ttctcccctt cctcccgccg gaccccgagc gcgggaagct
     1561 cctggtggag ctcaccccgt tccttcggga gctggccggg gccttcgaca ccaaggactc
     1621 ggtcctgatc ggggacctgc tggagtacga gatcacgcca cgaatgcaga ggctccttcc
     1681 gatgctggag cgcgcatcgt gatcctcctc cagacggggg tccagaacgt ctacacaagc
     1741 tccgagagct ccacggccat ctatttcggc ctcgccgtgg ccggtgtggc catcgtgctg
     1801 ctcgtcttcg cgttcatcaa ccggcgggtc cgggcacgca accccgaaga gacgcagcag
     1861 tattcctcct accagttccg acgcctggcc aagagctacg gtctgtcctc acagcacatc
     1921 gagatgctcg agcgcctggt gaccgtctgc aaggtgagac agcccatgct catcttctcc
     1981 agcgtgggcc tcctggatga caccctgaag aagggactgt actccctgga gggcgtacgg
     2041 gacgcgacgc ccgaagagag ggaaaaccag aaggccctga tcttcgagat caagcaggtc
     2101 ctcgagcagg gcggccgtcg cgtgggagcc gtaagctcca ccacggccct caagccgggc
     2161 caggagctga ccatccgggc ggggacgtcg cagttcgcca gccgggtcgt ctcgaacatg
     2221 agggactttc tgaccgtagc cgcgcccccc gtgaccacgg acgcgcaagc gcgatgggtg
     2281 cgcggcacgc agctggtggt ccacctctgg cgggatgggg acgccgggta cgccttcgag
     2341 tccaagatcc tgggctacga caccgtgaag ggcatcccct ccatactcat ccagcactcc
     2401 aagaccctgc ggaaggagca gcggcgcaag aaccgccgcc gggagctcct gcggccgtgc
     2461 ttcttctacc cgatccgggt cgctgaggtc ggccagggcc ggcaggccgc cgtgaaggcc
     2521 gtcgtggaac aggacaagag ggccctgggg cacgtcgtgg acatctcggc cggcggatgc
     2581 tccatccaga ctctcgtgcc gctggaaaag ggcaagctcc tcatggtgga gttcgagatc
     2641 ggccgcggga acaccatccg cacgtacggc aaggcggtgg ggctgtaccg ccggccgcgt
     2701 cgcggcggga tcatgcacgt gatgttcacc cgcgtcacgc ggcagtacct gaaccggatc
     2761 agcgagttcg tctacgactt cgccaggccg tccacggtgg gagaggcgcg cctgcagctg
     2821 gaccgacggc ccgcgatggg cggaacggtc tcccgcgccc gctgacccgc ggcgctcgcg
     2881 tttggcgcgg ccgcccctcg cccgcctgcc cgcgttgacc acccccaagc tttcctctac
     2941 cgtctgcgcg tgagcacgcg tcggattacc acccgggacg cagaggagac gctgcgtctc
     3001 ggccagcgga tgggggaaga gctggcggtc ctttcggacg gatccgtcct catcctcatc
     3061 ggcccgctcg gcgcgggaaa gaccgtgctg gcgaagggaa tcgccctggg cctgggcgtc
     3121 caggagccgg tggtcagccc cacctacacg atcgtat
//
LOCUS       DASRZS010000597         4370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1118796, whole genome shotgun sequence.
ACCESSION   DASRZS010000597 DASRZS010000000
VERSION     DASRZS010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118796"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VFH83_14495"
     CDS             complement(<1..808)
                     /locus_tag="VFH83_14495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HET6487633.1"
                     /translation="MKLEELTVTGMTCASCSARVERVVGKLDGVSSASVNLATEKLRL
                     EYDEGKTTVDQVRKAVENAGYGLVQEDRSKRVTIPIEGMTCASCVARIEKVVGRLDGV
                     KAVAVNLATERADVTYEPARVRLSSIKKTITDAGYTPREIDAEDAVDHDAERKEREIR
                     TLWRKFIVAASFSVPLLYVSLVHMVPGTRIPLPFFLDPMRVPLQYALAQVLLVIPAVI
                     AGNRFYRVGAKAVWHLAPNMDSLIAIGTAAAILYSLYNTVRVALGDAAAVE"
     gene            complement(910..1413)
                     /locus_tag="VFH83_14500"
     CDS             complement(910..1413)
                     /locus_tag="VFH83_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487634.1"
                     /translation="MHRSIRDLLFILVGLLVSAGLVWAARGDSTVRLNGDVAYLNTLG
                     RRSATAQSLDRSMMTIFRATEARLQTLKGAGMGEGDVAAALATASRLPGGITDASIDR
                     VLRSWRTGRTRGWPAIVKSLRVNADRVAVSVERVHPAPAGSVRTSAAHKRPVGSLLVQ
                     NGILTAP"
     gene            1473..2600
                     /locus_tag="VFH83_14505"
     CDS             1473..2600
                     /locus_tag="VFH83_14505"
                     /inference="COORDINATES: protein motif:HMM:NF015616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HET6487635.1"
                     /translation="MAQRLRLVAGGLLACAAILLLGQCVALDPGLRNGTSAADRAMAA
                     FNRAFLTGAAGVGNYRANTAGGWADFWKEAEMIEMAEDAYQRAGSPAYLRLVVELHDG
                     FVLRFGSDWSLNAYNDDIIWMCLACIRAYELTGNTGFLNQAKANFDRVWSRGFDGTLG
                     GGLWWNTSRGEKNTCITMPAAIMAYKLSVDLGDPSYLEKARALLAWQKDNLYDKATGR
                     VYDNERRDGVVDRTAYTYNAGTFIGAASFLGDVASARKALDYAREHLTVDGILKSEHA
                     THDQGGFKGIFCRWAYRFVRDEGLGSQYDPWFQRNVDAALARQDSRGLMDQDWSAATG
                     SGILDSWSCSSAVVLLQVTGVAETIVDLWPAEPPAGRPAHR"
     gene            complement(2605..>4370)
                     /locus_tag="VFH83_14510"
     CDS             complement(2605..>4370)
                     /locus_tag="VFH83_14510"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET6487636.1"
                     /translation="SHLDAAEAALMAGMIQAPSLYDPQANPDMALKRRDWVLDRMRET
                     HRVSDLDYTAAREEDLPSPREADGIRAGHFTDYVLSLAEGRTDITTTLDGSLQESVQA
                     LVSEHVRRYTRAGITNAAAVVLDNATGSILAMVGSRAYLEGETGFVNGALALRQPGST
                     LKPFAYALAFEKGWSPASMLADIETEYVSNDRTLYMPRNYSRTFRGPVLAKEALASSL
                     NIPAIRLVQQVGVADFLRELHDLGFVSLDKGADYYGLGLVLGNGEVSLLQLAGAYEAL
                     ARGGNYLEPSPFPVPAERKGRDVIDPKAAWLVTSILSDETMRIQAFGSNTPLMVGFPM
                     AIKTGTSGNWRDSWTAGYTQEFTIAVWSGDFEASPMNQVSGSTGAGPLFNSIARLMGE
                     RLGRLPTLPPAPPGVEVRVVCAESGDVPGEYCPRRMVVAVSADRARVPCTMHSLVRID
                     TRTGAPASPGTPSAFLSARVAYNLPAEFAQWLSESGKYTVPPREDPAKARPSDLAITR
                     PRPGDIYIVEPGYDLSTQSIELAALSRRRVASLDWYIDGTLVGEAAWPYSASWRLDRG
                     VHVVQARAGTTESPPVRFEVR"
BASE COUNT          695 a         1444 c         1498 g          733 t
ORIGIN      
        1 tctcgacggc ggccgcgtcg ccaagggcga ccctcaccgt gttgtagagg ctgtagagga
       61 tggctgccgc ggtgccgatc gcgatcaggg agtccatgtt cggcgccagg tgccagaccg
      121 ccttggcgcc gacccggtag aaccggttgc ccgcgatgac ggccgggatc accagcagga
      181 cctgggccag cgcgtactgg agtggaacgc gcatgggatc caggaagaag ggaaggggga
      241 tccgcgtgcc agggaccatg tgcaccaggc tgacgtagag caggggaacg gagaaggacg
      301 cggccacgat gaacttccgc cacagggtgc ggatctcccg ctccttgcgc tcggcatcgt
      361 ggtccacggc gtcttcggcg tcgatctccc gcggggtgta tcccgcatcc gtgatggtct
      421 tcttgatgga ggacagccgt acccgagccg gctcgtacgt cacgtcggcc cgctccgtcg
      481 ccaggttgac tgccacggcc ttcacgccat ccagcctgcc caccaccttc tcgatcctgg
      541 caacgcacga ggcgcaggtc atcccctcga tggggatcgt cacgcgcttg gagcggtctt
      601 cctggaccag cccgtagccg gcgttctcca ccgccttgcg gacctggtcc accgtggtct
      661 tgccttcgtc gtactccagg cggagcttct ccgtggccag attcacgctc gcggaggaga
      721 caccatccag cttgccgacc acccgttcca cgcgcgccga gcaggaggcg caggtcatgc
      781 ccgtcaccgt cagttcttcc agcttcatgg cttccatccc ctggcccctc ccctcgtggg
      841 gagggggtat aggaagagga taccgcgcgg ctgggtgatc gtcaaccgag gcttcgggcg
      901 ctgccaggct cagggggccg tcaggatgcc gttctgcacc agcaggcttc cgacgggccg
      961 cttgtgtgcg gccgaggtcc tgacggagcc cgcgggagcg gggtgaacgc gctctacgga
     1021 gaccgccacc cgatcggcgt tgacccggag ggacttgacg attgcgggcc agccccgggt
     1081 gcgtcccgtc ctccaggatc gaaggacgcg gtcgatgctg gcatccgtga tgcctcccgg
     1141 gagcctggac gccgtggcca gcgccgcggc cacgtctcct tctcccattc cggcgccctt
     1201 gagggtctgc aaacgcgcct ccgtggcgcg gaagatggtc atcatggagc ggtcgaggct
     1261 ctgcgccgtt gcggagcgcc gccccagcgt gttcaggtag gccacgtcgc cgttcagcct
     1321 caccgtggag tcgccgcgag cggcccacac caggccggcg gaaaccagaa gtcccaccag
     1381 gatgaagagc aggtcgcgaa tggagcgatg cattctctca cgcctccatc cagagcatcg
     1441 gcgaatccgg gcggatacgg tagtatgggg tcatggcgca gaggcttcgg ttggtggccg
     1501 gtgggctcct ggcatgcgcc gcgatcctgc tcctcggtca gtgcgtggcc ctcgatccgg
     1561 gccttcggaa cgggacttcg gcagcggacc gggccatggc ggcgttcaac cgggcgttcc
     1621 tgacgggggc ggctggcgtg gggaactatc gagccaacac ggcgggaggc tgggcggact
     1681 tctggaaaga ggcggagatg atcgaaatgg cggaggacgc ctatcagcgc gccggcagtc
     1741 ccgcctatct gcggctggtc gtcgagctcc acgatggctt cgtcctgcgg ttcggctcgg
     1801 actggtcgct gaatgcgtac aacgacgaca tcatctggat gtgtcttgcg tgcattcgag
     1861 cctatgagtt gacggggaac accggcttcc tcaaccaggc caaggcgaac ttcgaccggg
     1921 tctggagcag gggctttgac ggcactctgg gaggcgggct ctggtggaac acctcccgcg
     1981 gagagaaaaa cacctgcatc accatgccgg cagccatcat ggcgtacaag ctgtcggtgg
     2041 acctggggga tccctcctat ctggagaagg cgcgggcgct gttggcctgg cagaaggaca
     2101 acctctacga caaggcaacc ggtcgggtct atgacaacga gagaagggac ggggtggtgg
     2161 acaggaccgc gtacacctac aacgccggta ccttcatcgg ggccgcgagc tttctcgggg
     2221 acgtggccag cgcccgcaag gccctggact acgcccggga gcacctgacc gtggacggta
     2281 ttctcaagag cgagcacgcg acgcacgacc agggtgggtt caaaggcatc ttctgtcggt
     2341 gggcataccg attcgtccgg gacgagggac tcggttcgca gtacgacccg tggttccagc
     2401 ggaacgtcga tgcggcgctg gcccgccagg attcccgggg tctgatggac caggactggt
     2461 ccgcagctac cggttcgggg atcctggatt cctggtcctg ctcctccgcc gtggtgctcc
     2521 tgcaggtcac gggggttgcg gagacgatcg tcgatctctg gcccgcagag ccgcccgcag
     2581 gtcggcccgc acaccgatag ggccctaccg aacctcgaag cgcaccggtg gactttccgt
     2641 ggttccggct ctcgcctgta ccacgtgcac ccccctatcc agcctccacg atgcggagta
     2701 cggccaggct gcctcgccca ccagcgttcc gtcgatgtac cagtccaggg aggccacgcg
     2761 gcgccgggag agcgcggcaa gctcaatgct ctgcgtgctc agatcgtacc ccggctccac
     2821 gatatagatg tctcccggtc ggggtcgggt gatcgccagg tcggaggggc gcgctttcgc
     2881 cgggtcctcc cgtggcggca cggtgtactt gccgctctcc gaaagccact gggcgaactc
     2941 cgcgggcagg ttgtatgcca cgcgcgccga caggaaggcc gacggtgtgc cgggtgaagc
     3001 gggcgcgccg gtgcgcgtgt cgatccggac gagggagtgc atggtgcatg gcacgcgcgc
     3061 cctatccgcg ctcacggcca ccaccattcg ccgcgggcag tactcgcccg gcacgtctcc
     3121 ggattccgcg cagaccaccc gtacctcgac ccccggtggc gccggcggaa gggtggggag
     3181 acggcccagt cgctctccca tcaggcgagc gatggagttg aagagcggcc ccgccccggt
     3241 ggagccggag acctggttca tgggggaggc ctcgaagtct ccgctccaca ccgcgatggt
     3301 gaactcctgc gtataccccg cggtccacga gtctcgccag ttcccgctgg ttcccgtctt
     3361 gatggccatg gggaacccga ccatgagcgg cgtattcgat ccgaaggcct ggatccgcat
     3421 ggtctcgtcg ctcaggatgg aggtgacgag ccacgccgcc ttggggtcga tcacgtcccg
     3481 acccttgcgc tctgcgggaa cgggaaacgg agacggttcc aggtagttgc ccccgcgggc
     3541 cagcgcctcg tacgcgcccg caagctgcag gaggctcacc tcgccgttgc ccaggaccag
     3601 gcccaggcca tagtagtccg cgcccttgtc cagggagacg aaccccaggt cgtgaagctc
     3661 cctcagaaag tccgcaaccc ctacctgctg caccaggcgg atggcgggga tgttgaggct
     3721 ggaggcgagc gcctccttgg ccagcaccgg accgcggaag gtacgggagt agttccgcgg
     3781 catgtagagg gtgcgatcgt tgctcacgta ttccgtctcg atgtccgcga gcatcgacgc
     3841 cggcgaccag cccttctcga acgccaacgc ataggcgaag ggcttgaggg tggaccccgg
     3901 ctgacggagc gccagcgccc cgttcacgaa gcccgtctca ccctccaggt atgcccggga
     3961 cccgaccatc gccaggatgg atcccgtggc gttgtccagg accacggccg ccgcgttggt
     4021 gattccggcg cgcgtatacc ggcgcacgtg ctcgctgacg agggcctgga cgctttcctg
     4081 gagggacccg tccagggtgg tggtgatgtc cgtccttccc tccgccaggg acagcacgta
     4141 gtcggtgaag tgcccggccc tgatgccgtc cgcctctcgg ggggacggaa ggtcctcttc
     4201 ccgcgcggcc gtgtagtcca ggtcggagac acggtgcgtc tcccgcatcc tatccaggac
     4261 ccagtccctg cgcttgaggg ccatgtcggg gttggcctgc ggatcgtaga ggctcggcgc
     4321 ctggatcatc ccggccatca gcgccgcctc cgctgcgtcg aggtgggagc
//
LOCUS       DASRZS010000598         3865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4561151, whole genome shotgun sequence.
ACCESSION   DASRZS010000598 DASRZS010000000
VERSION     DASRZS010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4561151"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VFH83_14515"
     CDS             complement(<1..503)
                     /locus_tag="VFH83_14515"
                     /inference="COORDINATES: protein motif:HMM:NF019000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HET6487637.1"
                     /translation="MSDAIVPSGPDDIDLYVDEEDAAVRGRTWMIKDLPTVDWALRRL
                     AECNKELAQIDALEEIAIRRVRENAEKARRASRGGVAYMTFQLLAWMDRNKDLVVRGT
                     KKSRRFINGVLGYRGHGERLEVEDKGKVTEWLQTLPPESPMLRLKREAEMSAVQANFQ
                     ATGEIPPG"
     gene            complement(500..685)
                     /locus_tag="VFH83_14520"
     CDS             complement(500..685)
                     /locus_tag="VFH83_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487638.1"
                     /translation="MASKPVRLPEDDLETLLQAYALARRDVVMFLGGAPFSPLSEKSK
                     ALILCALRLAEYLTEAT"
     gene            767..982
                     /locus_tag="VFH83_14525"
     CDS             767..982
                     /locus_tag="VFH83_14525"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6487639.1"
                     /translation="MTTRKAERLGDRIRRVRHRRRLTLEAVAQKVGVTKSYMSWVETN
                     QGEPSLGNLRKIAKALEVKAGRLLGES"
     gene            1148..1438
                     /locus_tag="VFH83_14530"
     CDS             1148..1438
                     /locus_tag="VFH83_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487640.1"
                     /translation="MIAKELKIGDVVQMRTGQVRIAALYESHHGVEAVVCPARRADDK
                     GARILWFYPGDSLILTRRNTNPWVPILPRPKRERRDDDSGPEEEEGEPVSEA"
     gene            1772..2146
                     /locus_tag="VFH83_14535"
     CDS             1772..2146
                     /locus_tag="VFH83_14535"
                     /inference="COORDINATES: protein motif:HMM:NF025717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HET6487641.1"
                     /translation="MKYIYVAGPYSAATPEEIERNVWRAIDAGDEILKMGLWPMVPHE
                     HHYWHARHKHDYKDWTELDLAWIRRCDGVYRMHGASPGADREVELARFLGIPIFTTMA
                     SLRALADGVRKIREATKAWGLE"
     gene            2143..2361
                     /locus_tag="VFH83_14540"
     CDS             2143..2361
                     /locus_tag="VFH83_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487642.1"
                     /translation="MRLAPLHPDHFLVWVWQNLPPGDRLGTIPLQVDDVAFELREDRE
                     TVRRNYVRLAARVRRNQRLGKEPFDGIS"
     gene            2526..2786
                     /locus_tag="VFH83_14545"
     CDS             2526..2786
                     /locus_tag="VFH83_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487643.1"
                     /translation="MKRIACGTTVNGPSHWTTELRKASCYECARQAEGLTAERGVPLP
                     DNEPMSNAGTAALAVLVLGGGLLLLSVAAYMGIEVATNFRWD"
     gene            2903..3277
                     /locus_tag="VFH83_14550"
     CDS             2903..3277
                     /locus_tag="VFH83_14550"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6487644.1"
                     /translation="MILHYTDVVRLDSLPELLRAKLAPLTLPGMRLRGRLAMPEGVLV
                     VVALDHQGPCGWAAISRDGFIDVFVAPRSRRQGIGQGLITMLVPEARREWPDLDIRYQ
                     VEKGTPGFHFWRAMEQTMGGPR"
BASE COUNT          679 a         1237 c         1285 g          664 t
ORIGIN      
        1 ccgggcggga tctctcccgt agcctggaag ttagcctgga ccgccgacat ctcggcctct
       61 cgcttcaacc ggagcatcgg gctctccggg gggagcgtct ggagccactc tgtcaccttc
      121 cccttatcct caacctccag acgctccccg tggccccggt agcccaggac cccgttgatg
      181 aagcggcggc tcttcttcgt gccgcggacc accagatcct tgttgcggtc catccaggca
      241 aggagctgga aggtcatgta ggccacgcca ccccgcgatg cccggcgcgc cttctccgcg
      301 ttctcccgga cacgccggat cgctatctct tccagcgcgt ctatctgggc caactccttg
      361 ttgcactccg caaggcggcg gagcgcccag tccaccgtgg ggagatcctt gatcatccag
      421 gtgcggcccc ggaccgcggc atcctcctcg tctacgtaga ggtcgatgtc gtccggaccc
      481 gacggaacga tggcgtcgct cacgtagcct ccgtgagata ctccgcgaga cgcagggcgc
      541 agagtatcag ggccttgctc ttctcgctca ggggcgagaa gggcgcgccg cccaggaaca
      601 tcaccacgtc cctgcgagct agcgcgtagg cctgaagcag ggtttccaag tcatcctcgg
      661 gcagccggac cggtttcgat gccatgtccc tcctcctatc tcatccgcct gacactgtca
      721 agatgctctt gactctccct cctggatggt tctatagtcc atgaccatga caacgcgcaa
      781 agcagaacga cttggggacc gtatcaggag agtgcgacac cgtaggcggc tgaccttgga
      841 ggcggtcgcc cagaaggtgg gggtgaccaa gagctacatg agctgggtgg agacgaacca
      901 gggggagccg tcgctgggga atctccggaa gatcgcgaag gcgctggagg tcaaggctgg
      961 tcgccttctg ggggagtcgt gagccgccca ccggtacagt ggcggctcgt ggccctctcc
     1021 gccctcgcct ggtctgccgg gctcaacatg gggatggacg agtgggctcc atgcctggtc
     1081 ttcgcggcct gctggttggt gatgctggtc ctctggagcc aggagctatc ggagaactcc
     1141 cgtggtcgtg atcgcgaaag aactcaagat cggtgacgtc gtccagatgc ggaccggaca
     1201 ggtccggatt gcggcgctgt acgagtcgca ccacggcgtg gaggcggtcg tctgcccggc
     1261 gcgacgagcc gacgacaagg gggcccggat cctgtggttc tacccgggag attccctcat
     1321 cctgactcgg cgcaacacga acccctgggt cccgattctc ccgcgcccaa agcgggagcg
     1381 ccgcgacgac gattcaggac ccgaagagga agagggcgag ccggtgtcgg aagcgtgacc
     1441 gcggtggtag gattccacgt tgccttggac gggaggcgat cgtgttgcga ctgtcggtct
     1501 cggtcgtttc gctggccatc gtagccggcg tggttacctt ctacctatcc tcaccccggt
     1561 ctcgccctcc cttctccctt gggcgtaccg ggtccgtccg cgccgccgcc ccgcgggagg
     1621 gaagcacggg cggctctttc cttcccacct tctcccaggc aatcgaatcc gctcgctggc
     1681 aacgcatcca gcttgagcga gtcgaggcag ttctctggga gcacacggca gccgttctac
     1741 tccagcggca gcccgacacc ggagaccgtc agtgaaatac atctacgtgg cggggcccta
     1801 ctctgcggcg accccggaag agatcgagcg caacgtctgg cgtgccatcg atgccgggga
     1861 cgagatcctc aagatggggc tctggccgat ggtcccccat gagcaccact actggcacgc
     1921 ccggcacaag cacgactaca aggactggac cgagctggac ctggcctgga tccggcgctg
     1981 cgatggcgtt taccggatgc acggggcatc tcccggagcc gatcgggaag tagagctggc
     2041 ccggttcctc gggatcccga tcttcacgac catggcctcg ttgcgggccc tggccgatgg
     2101 agtacggaag atccgcgagg caaccaaggc ctgggggctg gaatgaggtt ggcgccgcta
     2161 cacccggacc acttcttggt ttgggtctgg cagaacctgc ccccgggcga ccggctcggc
     2221 acgatcccgc ttcaggtaga cgacgtggcc ttcgagctgc gcgaggatcg ggagaccgtt
     2281 cgcaggaact acgtgaggct ggcggcgaga gtcaggcgca accagcggct cgggaaggaa
     2341 ccgttcgatg gcatctcttg actcccccgc gcggcgacag gcgctcgttg ccaccgtggt
     2401 ctacgcggtg agcgccgtgg ttttggccgt tgtggcctgg atgttccaga cccttcagta
     2461 gagcgaggtt ctccgtgagt aactctccgc ctgtgcatct cctttggccg cgtaccggtt
     2521 cacccttgaa gcgcatcgcc tgcgggacaa cggtcaacgg accgtcgcac tggacgaccg
     2581 agctgcggaa ggcaagctgc tacgaatgcg cccggcaggc ggagggcctg accgcggagc
     2641 gcggagtccc tctcccggac aacgagccca tgtccaacgc gggcactgct gccctggccg
     2701 tgctcgtcct ggggggcggc cttctgctcc tgtcggtggc cgcgtacatg ggcatcgagg
     2761 tcgcgaccaa cttccgctgg gactagccca agtggctcac cgtggccacc ggagtgctga
     2821 tagcggcgtg gctgcccctc atcggtgtcg cggcgatctt cggcgcgatg gcagaccgta
     2881 ggtcccgcag gaggatggtc cggtgatcct gcactacacg gacgtcgtcc gcctggacag
     2941 tctcccggag ctactccggg ccaagctagc cccgctcacg ctccccggga tgcggctccg
     3001 ggggaggctg gccatgcccg agggagtgct ggtggtggtc gctctggacc accagggacc
     3061 atgcggctgg gcggcaattt ccagggacgg cttcatcgac gtgttcgtgg cgccgcgctc
     3121 ccggagacaa ggcatcgggc agggactcat cacgatgctg gtaccggaag ccagacgcga
     3181 atggccggac ctcgacatcc ggtaccaagt cgagaagggc acgccgggct tccacttctg
     3241 gcgcgccatg gagcagacca tggggggccc caggtgactg ccccggacgg gaccagggcc
     3301 tggcgcaagg gctggcggaa gcgctggcga gagggctcgt ccttcagggg cctgtctccc
     3361 gtagccaagg tcatcgtcca ctgggtcgaa gagaactgcg acgacgaggg caaggtcacc
     3421 acgactatgc ggcacctggc gaccatctct ggcggggatc accccaagac caaggtcaac
     3481 cgaatgaggg cctggaggtc tctccaggag gccgtgaggg ccgggatctt gcgaacggaa
     3541 gcgttacagc cagcgttaca ggaagcggga cagtgcccga ccgtaataac caggctaaac
     3601 tacaggcaat atcaggacca gtcggacgat agcgggacag gaagcgttac aggcatcgct
     3661 tcggaagcgg gacgatctgg tagaggaaag cagaagaaag cagaagatca ggatctgctt
     3721 cctgcgcctc ccggcgctgt tcagaacccc ccagccaacg gcttccgtgg tctccaggag
     3781 acactggtcc ggagctactt ggcagtgaag gggacgaagt acctctggca aggtgcgcga
     3841 gacacggaag ggttgaagcg catcc
//
LOCUS       DASRZS010000599         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2656095, whole genome shotgun sequence.
ACCESSION   DASRZS010000599 DASRZS010000000
VERSION     DASRZS010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2656095"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1082)
                     /locus_tag="VFH83_14555"
     CDS             complement(21..1082)
                     /locus_tag="VFH83_14555"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HET6487645.1"
                     /translation="MVLPAETLKDIGIRLFAACGASARDAETVASELVEASLMGLDSH
                     GVMRYAQYVDEVLAGTIQPKATPRIERETATTAVVDCGSGFGIASACRMVEIVIDKAR
                     AADLACVVSTRCQHVGRLGSYAQKVARAGLVGLAAANSSRQGHWVAPWGGREGRLATN
                     PLAFAAPTSGDPLIVDMSTSMIAEGKIRILKQNGAPVPPGSILDGNGNPTTDPNLFYA
                     EPHGTILPFGSPTLGYKGFGLGLLVEILGSSLAGVPLTSAGGKSEYTNGLFLLAINPE
                     PLAGPQGLRTLVDELCAYVTSSRPAPGHREVVVPGALDFRTRARRLEEGIPVAEGTWR
                     EIERVAGRLSVTLPPVAGG"
     gene            complement(1082..1903)
                     /locus_tag="VFH83_14560"
     CDS             complement(1082..1903)
                     /locus_tag="VFH83_14560"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET6487646.1"
                     /translation="MSRMERIEGFRRDLESGKICLGASVGFTDPLVSDALADSVDFLW
                     IDLEHAAMSPEALSAHLLAARSRSTPAIVRVTGSATPFIKPVLDAGAEGIIVPQVLSV
                     SEVKSIVSDCRYVPMGKRGFGPRVPGNYGRDAGPDYIREANESLFVAVMVETNEALEA
                     VEDIVRIEGLDSLVIGPMDLSASQGMLGEVEHPRMLKAYERIIGAARGAGLFVGAGMG
                     INLGLCRRMIDMGVQWIQLAGDSDYLIYAFDGLARTIRSGLADGRSPGGTEGGPL"
BASE COUNT          354 a          765 c          698 g          380 t
ORIGIN      
        1 gcgtcgcggc ggatacgtgc tcacccgccc gcgaccggcg gcagggtgac gctcaggcgc
       61 ccggcgactc gctcgatctc ccgccacgtg ccctcggcca cggggattcc ttcctccagc
      121 ctcctggcgc gcgtcctgaa gtccagcgcc cccggcacca cgacctctcg atgtcctggc
      181 gccggacggg acgacgtcac gtacgcgcag agctcgtcta ccagggtccg cagcccttgc
      241 gggccggcaa gcggctcggg gttgatggcg agcaggaaca gaccgttcgt gtactccgac
      301 ttccctccgg ccgacgtcag cggcacaccg gccaggctgc ttcccaggat ttccaccagg
      361 agccccaggc cgaatccctt gtagccgagg gtcggtgatc cgaacggaag gatggtgccg
      421 tgaggctcgg cgtagaagag attcgggtct gtggtgggat tgccgttgcc gtccaggatg
      481 gatccgggcg gcaccggggc tccgttctgc ttcaggatcc ggatcttccc ttcggcgatc
      541 atggacgtgg acatgtccac gatcagggga tccccgctgg tcggcgcggc aaacgccaac
      601 gggttggttg ccagtcttcc ttcgcggcct ccccacggtg ccacccagtg cccctggcgc
      661 gagctgttgg cggccgccag ccccaccagg ccggcgcgcg caaccttctg cgcgtaggac
      721 ccgagccgcc ccacgtgctg gcaccgcgtg ctcaccacgc aggccaggtc cgcggcgcgc
      781 gccttgtcga tgacgatctc caccatgcgg cacgcgctgg cgatgccgaa gcccgacccg
      841 cagtccacca ccgccgtggt tgccgtctct cgctcgatcc gcggcgtcgc cttcggctgg
      901 atggtgccgg ccaggacctc atcgacgtac tgggcgtagc gcataacccc gtgggagtcc
      961 aggcccatca ggctggcctc caccagctcg gaggccacgg tctccgcgtc ccgggcggag
     1021 gcgccacacg ccgcgaacag gcggatcccg atgtccttca gcgtctccgc ggggaggacc
     1081 atcagagcgg gcccccctcg gttccccccg gcgatctgcc gtccgccagc ccggaccgga
     1141 tggttcgtgc cagcccgtcg aaggcgtaga tgagatagtc ggaatcgccg gcaagctgga
     1201 tccactggac gcccatgtcg atcattcgtc gacacaagcc caaattgatg cccatgcccg
     1261 cgccgacgaa gagcccggcg cctcgcgcag cgccgatgat cctctcgtat gccttgagca
     1321 tcctggggtg ctcgacctcg cccagcatgc cttgagaggc cgagagatcc atgggtccga
     1381 tcaccaggga gtccaggccc tcgatcctca cgatgtcctc gaccgcttcg agggcttcgt
     1441 tggtctccac catcaccgcc acgaagaggc tctcgttggc ctctcggatg taatcgggtc
     1501 cagcgtccct tccgtagttc cctggaaccc ggggaccgaa gcctcgcttg cccatgggga
     1561 cgtagcgaca gtcggagacg atcgacttca cctcgctcac actcaagacc tgggggacga
     1621 tgatcccttc ggcgccggcg tccagcaccg gcttgatgaa cggggtcgcc gagccggtca
     1681 ctcggacgat ggccggtgtg cttcgtgacc gagccgcaag gagatgcgcg ctgagcgcct
     1741 cggggctcat ggcggcgtgc tcgaggtcga tccagaggaa gtccacggag tccgccagtg
     1801 cgtcgctcac cagggggtcc gtgaagccga cggaagcgcc gagacagatc ttgccagact
     1861 ccaggtccct tcggaaaccc tcgatccgtt ccattctgct catggtccag cccccttgga
     1921 tgctgattct acatgaacgt tcacgctatc caggcagggg tggaatgtca acacagtcgt
     1981 tgacatcgca tgcccggagc gtgtaatcgc tcgaagtaga acgttcatgt gaacggtgtg
     2041 gcgaaccggc tagtcacggt ttctttgggg ccaactctag agagaacggc gtcgtcggag
     2101 tcgctcagga gttcatcgcc cggatctcct cctgcgtgcg ccagggaagc acgcgcagct
     2161 tcacgccacc atccacgacc aggttggttc cggtggt
//
LOCUS       DASRZS010000600         8311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_339114, whole genome shotgun sequence.
ACCESSION   DASRZS010000600 DASRZS010000000
VERSION     DASRZS010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8311
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339114"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1464)
                     /locus_tag="VFH83_14565"
     CDS             complement(82..1464)
                     /locus_tag="VFH83_14565"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HET6487647.1"
                     /translation="MKGALRFLVVVLMTSAAGSAFAASFDVGDLLSNSTGVQGSVSGS
                     PSLVQNDLAQLWMQAAFGDSIILNGQLGLTYANAQTGFGNALSPQVLAVDLNTLNLQA
                     TFPQPQPGVSLYQYTLGRATVSDFSALVLSQNADGLSMTFGFPVASLNVAMAYTGLLL
                     KPSTTIVLSRADAADSVNPAVLFAPPRLLEALQLTFPAVFGQRIILQAIAQEDMRGTV
                     LDLATPAKTSEIVKLGQTTQDPTLGGPVSTQYFGLELDGPIVGALYYDAYGYLQTGQF
                     LQYTDGSYQQAGQLAYLASIGLRYYAPDLLFSAVGAKLLFASGDASATTVVEGKAAGT
                     SSAFTPITNATIAYVFSPQLTNIAYGEVSYSLKPLTAIRAALADSLQTQVVADVFLRP
                     TPGATSAPGIVAGNTDLYLGTEVDLVVTARPVSDIGLTLTAGVFMPGSAFGAAPAIQY
                     KTGLEVSISI"
     gene            complement(1461..1919)
                     /locus_tag="VFH83_14570"
     CDS             complement(1461..1919)
                     /locus_tag="VFH83_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487648.1"
                     /translation="MRRLHVILVPIVFVLFAASLHAQSNQIVDRLLDQKVASFGDAAY
                     LILSAAGTIPPDATPADAVAAVESAKLLSGHHTEAQPITLGEVCYLIMKTENIKGGLF
                     YTLFPGPRYATRELVYLRLVRGVTHPGRTVSGEEVIRMLGDAMDLKGGQS"
     gene            1995..4697
                     /locus_tag="VFH83_14575"
     CDS             1995..4697
                     /locus_tag="VFH83_14575"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HET6487649.1"
                     /translation="MQQRRTDLAVTVGIVLVFGVLGGIGALKALDGRVYDMFLHVRPP
                     IAENPSILLLDVDDPAISKVGVWPWSRDVMADGLVLMREFGAGYAVFDIEYLNPSPRG
                     VDSFVLRNAVPDAFDQEFSQIQTNVQQLVDAIRTGSIPSRDVPKYVSDLEGLTVQSKG
                     KLLDTVKSVERDNDTYLGEAARFFGHAFMTVAAPDLPDDSASKETVDYAMANLALTNV
                     TVRADPTRAAPSLVPAIVPILKGDAGAGFPNVVVDPDGVRRRIDLIKSYHGKYFAQLA
                     FRPLLAWLGNPPIELDRRRILLRGATVPGKGKTDLSIPLTSDGQLLINWPHADYLHSF
                     RHLTFYELVLHKNLESDLLYNLKLMNQSGYLSYDTGDFQLLDGYTYAEGIKKDVLAGG
                     SLSQVDEYVQTRAAFFDAVGKFLNGDAETRILKDIDANLARKGLSAADRANVQAIRDQ
                     VPATFESTRGVYSDLMKVRQVLSKELPGAYCIIGNTATSTTDIGVNPFSGEYPNVGTH
                     ASVINTILQEQFLNATPWWVSLILALLFALIITMVTARLNPLRSTIVGVGIVVLVIAA
                     FAVFFRFTGIYLAPVSPVASVFLTFVALTAIKFLRAEQEKSFVRGAFARYLSSDVIND
                     LLANPDKLKLGGEKKVLTAMFTDVRGFSTISEVMDPTELVRLLNLYLTEMSDIIMDLR
                     GTIDKYEGDAIISFFGAPLDFADHARRACLAAVRMKRAERVLNERFITEKLAPSSLLT
                     RVGINTGEMVVGNMGTVQKMDYTIMGNAVNLASRLEGINKRYGTWVIMSEAVQEGIGN
                     GFVTRRLDRVRVVGINTPVRLFELVEEAGHVDGSAREALGIFEEGLKLFEAKDWTRAE
                     SIFENVLKIIPDDGPAKSYIKQCQEFAAKAPPANWDGVFNLTSK"
     gene            complement(4678..5898)
                     /locus_tag="VFH83_14580"
     CDS             complement(4678..5898)
                     /locus_tag="VFH83_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET6487650.1"
                     /translation="MFSSRTPRPHTGNRLTRAIAQLRAQGVDLIDLTESNPTLCGIRY
                     DTEGIALALAGAAAAPYEPDPQGLAAARAEVARYYAERGMRVDPASLVLTSGTSEGYA
                     HLFTLLAEPGDEVLVPTPGYPLLEVLTSIGGIGLIHYRQSYDDGAGWGVDIERLRGSV
                     STRTRAIVVVSPNNPTGAFLKTRELEQMGAICREHDLALIVDEVFSDYGVGSDPGRVT
                     SAIGHVACLTFVLNGLSKVSGLPQMKLAWIHVSGPSELRAEAVDRLAFVADAYLSVGT
                     PVQRAVSKLLLERHSAQRQIQERVIANDAALRTLLEGRAGCRVLRREGGWYGVLRLPD
                     HLVDEDVAIALLEQEHVIAHPGYFYDFPAGAAFLVLSLLPEEDTFRDGILRMRNRLGS
                     AEGPAGASATSTSG"
     gene            5985..6722
                     /locus_tag="VFH83_14585"
     CDS             5985..6722
                     /locus_tag="VFH83_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET6487651.1"
                     /translation="MVVSCTVTEKIDYEELLPHELAQRLGHRPVGYLPLGTLEWHGVQ
                     NALGADFIQARGIFRRAAQRFGGVVLPPLWVGPDRIACQESGPDLIGMDYADTTTPNR
                     QLPGSCYWIPKGLFLSLVEGVLAQARRAGFRCIVADGHGPSRNAWAEMVPTWEKQFGL
                     LLVSTVRDFADRWPTQQDHAGRNETSIMLAVEPRLVELSLLPKSRSEWPQGVAGEDPR
                     DATADHGEELIEKALGLLGGRLEQLGI"
     gene            complement(6756..7823)
                     /locus_tag="VFH83_14590"
     CDS             complement(6756..7823)
                     /locus_tag="VFH83_14590"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET6487652.1"
                     /translation="MAIFVPAVSIPTSRFLLALMLASLGAASTVRADSSSVPADGPIS
                     RTQNVLWTTCTVTLYDHGTPQILDASFRRLQEIHDRMSTNVPGSLLDAVAAQAGKTPV
                     HVTDDVFLVAEKALEFARLTDGLFDPTVGPLVKAWRFDKDDPRIPADSVIHDALALVG
                     WKDVVLDDAARTIYLARPGMQLDFGGLIKGYAADEVAEILRNQGVKSAIIDLGGDIYA
                     LGGEPSGAPWRIGIQNPDAERGASFAIASITSQSIVTSGVYEHYFQVNGKRYSHIINT
                     RTGYPVDNGLTSVTVISDASVDADGLGLSLFCLGVKDGLALGEKLGLGVVMVDSDHRV
                     YATPKARDILAITDPDFRYAD"
     gene            complement(7840..>8311)
                     /locus_tag="VFH83_14595"
     CDS             complement(7840..>8311)
                     /locus_tag="VFH83_14595"
                     /inference="COORDINATES: protein motif:HMM:NF013682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004571 - mannosyl-oligosaccharide
                     1,2-alpha-mannosidase activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HET6487653.1"
                     /translation="LERVFGALECYFPGLLVLSGNTAMARALEDSCFAMWNRFGVEPE
                     QYDYSTGTAVSPQYYLRPEIMESAFYLYRDTADPKYLTMGRVFLSALQSFCRTDAGYT
                     ELSDVVSKTKADHMPSYFLAETLKYLYLLFAPPQTLDLKKVVLTTEAHPLRKDW"
BASE COUNT         1490 a         2674 c         2750 g         1397 t
ORIGIN      
        1 agggactggg cgcctagaga agctgccatc gccagcccga gcaagaggac aaaaaacaag
       61 cgacgcttca taaggtcctc cttaaatgct gatggagacc tcaagccctg tcttgtattg
      121 gatggccggg gccgcgccga aggcgcttcc tggcataaac acccccgcgg tgagggtgag
      181 ccctatgtcc gagaccggtc tcgcggtgac caccaggtcc acctccgtgc ccagatagag
      241 gtcggtgttt cccgccacga tgcccggagc gctggtagcc cccggagtcg ggcggaggaa
      301 cacatccgcc accacctggg tctggagact gtcagccaat gcggcccgta tggccgtcag
      361 aggcttcagc gagtagctca cctcgccata ggcgatgttc gtgagctgag gggagaacac
      421 gtacgcgatc gtcgcgttcg tgatcggcgt gaacgccgac gacgtgccgg cggccttgcc
      481 ctcgactacc gtcgtggcgc tcgcgtcccc gctcgcaaac aggagtttag caccgaccgc
      541 cgagaaaagc agatccggcg cgtaatatcg caagccaatg cttgcaagat acgcgagctg
      601 cccggcttgc tggtaagagc catcggtgta ttggagaaac tggccggtct ggagatagcc
      661 gtacgcatcg tagtagagag caccaacgat cggaccatcg agctccagcc cgaagtactg
      721 cgtgctcacc gggccgccga gtgtgggatc ctgcgtggtc tggcccagct tcacgatctc
      781 cgacgttttc gccggcgtgg ccaggtccag caccgtgccc cgcatatctt cctgggcgat
      841 ggcctggagg atgatccgct gtccgaacac ggccgggaac gtgagttgca gcgcctccag
      901 caggcgcggg ggtgcgaata gaaccgccgg gttcacggaa tccgccgcgt cggcgcgcga
      961 gaggacgatc gtcgtggaag gcttcagaag aagccccgta tacgccatgg ccacgttgag
     1021 cgaggcgacc gggaagccga acgtcatcga caggccgtcc gcgttctggc tgaggaccag
     1081 agcgctgaag tccgacaccg tcgctctccc cagcgtgtac tggtagagcg agactccggg
     1141 ttgcggctgc gggaaggtgg cttggaggtt cagcgtgttg aggtccaccg cgaggacctg
     1201 cgggctgagg gcgttgccga acccggtttg cgcgttcgca taggtgaggc cgagctggcc
     1261 gttgaggatg atcgagtcac cgaaggcggc ctgcatccag agctgcgcga ggtcgttctg
     1321 cacgagggag ggggatccgc tcacgctccc ctggacgccc gtgctgttgg agaggaggtc
     1381 gcccacgtca aagcttgccg cgaacgcgga gccggcggcg ctcgtcatga gaaccaccac
     1441 cagaaagcgc agcgcgccct tcatgactgg cctcccttca gatccatcgc gtccccgagc
     1501 atccggatca cctcttcccc cgacacggtc cggccggggt gggtcacccc acgcaccagc
     1561 cgaaggtaca cgagctctcg ggtggcatag cgcgggcccg ggaagagcgt gtagaacagg
     1621 cctcccttga tgttctccgt cttcatgatc aggtagcaga cctcacccag ggtgatcggc
     1681 tgggcctccg tgtggtggcc cgacaacagc ttcgccgatt ccaccgccgc cacggcgtcc
     1741 gccggcgtgg cgtccggcgg aatggtgccc gcggcggaga ggatcaggta ggcggcgtcc
     1801 ccgaaggatg ccaccttctg atccaggaga cggtccacga tctggttgga ttgcgcgtgg
     1861 agcgaggcgg cgaacagcac gaagacgatc gggacgagaa tcacatgcaa tcgtctcata
     1921 cctttgactc ctgtcgggcg cagaatggtt atagtatact gactgcagca ggggtctaca
     1981 attaggaggc gcggatgcaa cagcggagaa ccgaccttgc tgtcaccgtc ggcatcgttc
     2041 tggtgttcgg ggtcctgggt gggatcggag cgctgaaggc gttggacggc cgggtctacg
     2101 acatgttcct gcacgtccgc cctcccattg ccgagaatcc ttccatcttg ctgctggacg
     2161 tcgacgaccc ggccatctcc aaggtggggg tctggccgtg gagccgggac gtgatggcgg
     2221 acggcctggt cctgatgcgc gagttcggcg ccggctacgc cgtgttcgat atcgagtacc
     2281 tgaacccctc tccccgcggc gtggactctt tcgtgcttcg aaacgcggtt cccgacgcct
     2341 tcgaccagga gttctcccag atccagacca acgtccaaca gctcgtggac gccatccgga
     2401 ccgggagcat cccttcccgt gacgtcccca agtacgtttc cgatctggag gggctcaccg
     2461 tgcagagcaa gggaaagctg ctggatacgg tgaaaagcgt ggagcgggac aacgacacgt
     2521 acctggggga ggcggcgcgc ttcttcggcc acgcgttcat gaccgtggcc gcgccggacc
     2581 tgccggatga ctccgcctcc aaggagaccg tggactacgc gatggccaac ctggccctca
     2641 ccaacgtcac cgtgcgcgcg gatcccacgc gcgcggcgcc gtccctggtg ccggccatcg
     2701 tcccgatcct caagggcgac gcgggtgccg gctttccgaa cgtcgtcgtg gacccggatg
     2761 gagtccgacg gcggatcgac ctgatcaagt cgtaccacgg caagtacttc gcccagctcg
     2821 ccttccgccc gctgctcgcc tggctgggca atcctccgat cgagctggac cggcgccgca
     2881 tcctgctgcg gggcgcgacg gtgccgggga aaggcaagac ggatctctcc atcccgctta
     2941 ccagcgacgg gcagctcctc atcaactggc cgcacgcgga ctacctgcac agcttccgac
     3001 acctcacgtt ctacgagctc gttctgcaca agaacctcga gagcgacctc ctgtacaacc
     3061 tgaagctcat gaaccagtcc ggctacctga gctacgacac gggagatttc cagctcctgg
     3121 acggctacac ctacgcggag gggatcaaga aggacgtcct ggccggcggg agcctctccc
     3181 aggtcgatga atacgtccag acccgggccg cgttcttcga cgcggtgggg aagttcctga
     3241 acggagacgc cgagacgcgg atcctcaagg atatcgacgc gaacctggcg cgcaagggtc
     3301 tgagcgcggc cgaccgggcc aacgtgcagg ccatccgaga ccaggtcccc gccaccttcg
     3361 agagcactcg cggtgtctat tccgacctga tgaaggtccg acaggtgctc tccaaggagc
     3421 tgcccggcgc gtactgcatc atcggaaaca ccgccacctc caccacggat atcggggtca
     3481 atccgttctc cggcgagtac ccgaacgtgg ggacccacgc ttccgtgatc aacacgatcc
     3541 ttcaagagca gttcctgaac gcgaccccct ggtgggtgag cctgattctt gccctcctct
     3601 tcgcgctcat catcacgatg gtcacggcgc gcctgaaccc gctgcgctcc acgatcgtgg
     3661 gcgtcggcat cgtggtcctg gtgatcgcgg ccttcgcggt gttcttccgg ttcaccggca
     3721 tctatctggc tcccgtgtct ccggtggcat ccgtattcct gaccttcgtc gccctcaccg
     3781 ccatcaagtt cctgcgggcg gaacaggaga agagcttcgt gcgcggcgcc ttcgcccgct
     3841 atctctcctc cgacgtcatc aacgacctgc tggccaaccc ggacaagctg aagcttggag
     3901 gcgagaagaa ggttctcacc gccatgttca cggacgtgag agggttctcc accatctccg
     3961 aggtcatgga tcccacggag ctggtccgtc tcctgaacct ctacctcacc gagatgagcg
     4021 acatcatcat ggacctgcgg ggcacgatcg acaaatacga gggggacgcg atcatctcct
     4081 tcttcggcgc tcccctggat ttcgccgacc acgcgcggcg ggcctgcctg gccgccgttc
     4141 ggatgaagcg ggccgagcgg gtgctcaacg aacggttcat caccgagaag ctcgctccct
     4201 ccagcctgct cacgcgggtg ggcatcaaca cgggtgagat ggtggtgggc aacatgggaa
     4261 ccgtccagaa gatggactac acgatcatgg gcaacgccgt gaacctggcc tcccgcctgg
     4321 aagggatcaa caagcgctac ggcacctggg tcatcatgag cgaggccgtg caggagggga
     4381 tcggcaacgg tttcgtgaca cgcaggctgg accgggtccg ggtcgttggg atcaacacgc
     4441 cggtgcggct cttcgagctg gtggaggagg ccgggcacgt ggacggctcc gcccgggaag
     4501 cccttggcat cttcgaggaa ggcctgaagc tgttcgaggc aaaggactgg actcgggcgg
     4561 agagcatctt cgagaacgtc ctgaagatca tccccgacga tggtccggcc aagtcctata
     4621 tcaagcagtg ccaggagttc gccgccaaag cgccgcccgc aaactgggac ggcgtgttca
     4681 acctgacgtc gaagtagcgg aggcccccgc cgggccttcg gcagagccaa ggcgattgcg
     4741 catcctcagg atcccatccc ggaaggtgtc ttcttccggg aggaggctca gcaccaggaa
     4801 cgcggcaccg gccgggaaat cgtagaaata tccggggtga gcgatcacgt gctcctgctc
     4861 caggagcgct atcgccacgt cctcgtccac cagatggtcc gggagccgga gcacgccgta
     4921 ccacccgccc tcccgccgca ggacccggca tcccgcccgc ccctccagga gggtccgcag
     4981 ggccgcgtcg ttggcgatga cgcgctcctg gatctgtcgc tgggccgaat gccgctcgag
     5041 caggagcttc gacaccgccc gctgtacggg agtgccgacg gagaggtacg catccgccac
     5101 gaatgccagg cgatccacgg cctccgcgcg gagctcggac gggccgctga cgtggatcca
     5161 cgcgagcttc atctgcggaa gcccagagac ctttgagagc ccgttgagca cgaaggtgag
     5221 gcaagcgacg tgccctatcg cgctcgtcac gcgaccggga tccgagccga ctccgtagtc
     5281 ggagaagacc tcgtccacga taagcgcgag gtcgtgttcg cggcagatcg cgcccatctg
     5341 ttcgagctcc cgggtcttga ggaaagcccc cgtggggtta ttggggctca ccaccacgat
     5401 ggcgcgggtc cgggtgctga cgctccctcg aagccgctcg atatccacgc cccacccggc
     5461 gccatcgtcg tagctctggc gatagtggat caacccgatc ccgccgatgg aggtcagtac
     5521 ctccagcaac ggatagccgg gcgtcgggac cagcacctcg tcgcctggtt ccgccagcag
     5581 cgtgaagagg tgggcgtagc cttcgctcgt gccggaggtg agcaccaggg aggcagggtc
     5641 cacccgcatc ccgcgttcgg cgtagtagcg cgccacctcc gcgcgcgccg cggcaagacc
     5701 ctggggatcc ggctcgtagg gcgcggcggc ggcaccggcc agggccagcg caatgccctc
     5761 cgtgtcgtag cggatcccac acagcgtggg gttggattcg gtcaggtcga tcagatcgac
     5821 gccctgggcg cgcagttggg cgatggcccg ggtgaggcga ttaccagtat ggggtcgcgg
     5881 tgtgcggctg gagaacacgg ctggctcctg cgggcaactg aagcacggaa cccggcggcg
     5941 ggaaaagggg gcagcggcag gtccctcgcg agccaggcgc atccatggta gtctcctgca
     6001 ccgtgacgga gaagatcgac tacgaggagt tgcttcctca cgagcttgcg cagcgtctgg
     6061 gtcatcggcc cgtggggtac ctgcccctgg ggacactgga gtggcacggg gtgcagaacg
     6121 ccctcggcgc ggacttcatc caggcgcgcg ggattttccg cagggccgca cagcggttcg
     6181 gcggcgtggt gctgccgccg ctctgggtcg gaccggaccg gatcgcctgc caggagagcg
     6241 gtcctgacct catcggcatg gactacgcgg acaccaccac tcccaaccgc cagctccccg
     6301 gaagctgcta ctggataccc aagggtctct tcctctccct ggtagagggg gtgctggcgc
     6361 aagcccggcg cgccggcttc cgctgcatcg tggcggacgg ccacggtccg tcccgcaacg
     6421 cctgggccga gatggtgccc acctgggaga agcagttcgg gctgctcctg gtgtccacgg
     6481 tccgggactt cgcggatcgc tggcccaccc agcaggacca tgccgggcga aacgagacgt
     6541 cgatcatgct cgccgtggag cctcggctgg tcgagctttc tctccttccg aagagtcgat
     6601 cggagtggcc gcagggcgtc gcgggagagg accctcggga tgccaccgcg gaccatggcg
     6661 aagagctgat cgagaaggcg ttgggtctcc tgggtggaag gctcgagcag ctcgggatct
     6721 agcgagaccg gacctcgacg cttcggaccc gcaggtcagt ccgcgtagcg gaagtccggg
     6781 tccgtgatgg ccaggatgtc ccgcgccttg ggcgtggcat acacgcggtg atccgagtcc
     6841 accataacca cacccaggcc cagcttctcc cccaacgcca gaccgtcctt cacgcccaga
     6901 cagaacaggg agagacccag tccatccgcg tccacggagg catcggagat caccgtgacg
     6961 gaggtcagcc cgttgtccac ggggtagccg gttcgggtgt tgatgatgtg gctgtagcgc
     7021 ttcccgttca cctggaagta gtgctcatag acaccggagg tgacgatgga ctggctcgtg
     7081 atgctggcga tggcgaagga tgcgccccgc tcggcatccg gattctggat cccgatgcgc
     7141 cacggcgcgc cggagggctc tcctcccaac gcgtagatgt caccgccgag gtcgatgatc
     7201 gcggatttca ccccttggtt gcggaggatc tcggcgacct catcggcggc gtatcccttg
     7261 atcaggccgc cgaagtccag ttgcatgccg ggacgcgcca gatagatggt cctggcggca
     7321 tcgtccagga ccacatcctt ccagcccacc agagccagag cgtcgtggat gacggaatcg
     7381 gcggggatcc gcggatcgtc cttgtcgaat cgccatgcct tgacgagggg gccgaccgtg
     7441 gggtcgaaga gcccgtcggt gagacgggcg aactccagcg ccttctcggc gaccaggaac
     7501 acgtcatcgg tgacatggac cggcgtcttc cccgcctggg cggccacggc atccagcagg
     7561 gaaccgggca cgttggtgct catgcggtca tggatctcct ggagccgacg gaagctggcg
     7621 tccaggatct gcggtgtccc gtggtcgtag agggtgacgg tacaggtggt ccagagcacg
     7681 ttctgggtcc gagagatcgg gccgtccgcc gggacggacg atgaatcggc acggacggtc
     7741 gatgcggcac ccagggatgc cagcataagg gccaggagga atcgtgatgt cgggatagac
     7801 acggcaggca cgaatatcgc cacgccgtct cgctcctggc taccagtcct tgcgaagggg
     7861 atgggcctcc gtggtgagca cgaccttctt cagatccagg gtctgcggag gcgcgaagag
     7921 gaggtacagg tacttcaggg tctcggcgag gaagtaactg ggcatgtggt cggccttcgt
     7981 cttgctcacg acgtccgaga gctccgtata cccggcgtcg gtccggcaga acgactggag
     8041 agcgctaagg aatacgcgcc ccatggtcag gtacttcggg tcggcggtgt cccggtagag
     8101 gtagaaggcc gactccatga tctcgggtcg caggtagtac tgcggcgaga ccgcggttcc
     8161 cgtactgtaa tcgtattgct cgggctccac gccgaaccgg ttccacatcg cgaagcagga
     8221 gtcttccagg gctcgcgcca tggccgtgtt ccccgagagc accagcagcc cggggaagta
     8281 gcattccagc gccccgaaca cgcgctccag g
//
LOCUS       DASRZS010000601         4283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3038832, whole genome shotgun sequence.
ACCESSION   DASRZS010000601 DASRZS010000000
VERSION     DASRZS010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3038832"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VFH83_14600"
     CDS             <1..1082
                     /locus_tag="VFH83_14600"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HET6487654.1"
                     /translation="MDFMIALGSHTPMSDPAIDARFGLSPGDREKLFPGSRFLNHRWD
                     LPDTLEKIGTISAREVRDLTKGLMDESADVVINRAIREYDLILILGPVFPHEVIGFSG
                     GNKYLFPGISGGEFLSFFHWMGALITCPEIIGFKNTVVRATVDRAAAMVSVNRWCLAG
                     VVGPGGKLAGLFAGTPEEAWSQAADLSAQIHVVYKERPYRTVLGIAPTMYDELWVAGK
                     VMYKLEPVVAEGGTLIIHGPHVKEVSRTWGSHLLRTGYHVRDWFLAHPELGTGVPRAV
                     LAHSTHVRGIGSVVAGVEKPRIQVVLATGIPEQVCRQIGLGYCDPGSIHPSEFAGRED
                     EGVLLVDHAGEVLHRLEQDRARMKS"
     gene            1079..1837
                     /locus_tag="VFH83_14605"
     CDS             1079..1837
                     /locus_tag="VFH83_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487655.1"
                     /translation="MNGHTLVVLDFDGVICDTIDECFVSSWIAYHSLYHNEALRPPAR
                     TIRNEFARLRPFVRSGEDFVRIQEILATGARVTDQAGFDELTRQAGEESRKRFREVFY
                     QARTEMLERDRDGWLALSRIYPHVILAFAHLPREVPLYVLSTKKREFVEEIVRAEGIQ
                     IPSEKILWSDREPKLATVHRLLEEGRYPQAILVDDQIDYLATNADPRIRVYLASWGYV
                     QKEWLCEPLKVPVLAPQDLKPLLEEALAPARTAS"
     gene            complement(1874..3037)
                     /locus_tag="VFH83_14610"
     CDS             complement(1874..3037)
                     /locus_tag="VFH83_14610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6487656.1"
                     /translation="MEAFLLVFAAISAVILCFHSILALGCLLNLARERRLARVGASSG
                     AGGPTGALIAEVIVAVRNEEKLLPRLLSALGAQTRRDCLFLLVDDRSTDATPQLLERF
                     CRDLGSRARLLRAEREPEGLTGKQAALDLAFSQARGDVLLFTDGDCSPPPTWVEEMMR
                     HFADPRTGVVLGRIELESRKGFLLRFQGFEQPLLNQYNFGSLGVGLPTGGFGNNMAVR
                     AVAVRQTGGFQALGFSVTEDAMLLDAVCRGAGWKGAVCTASGAAMLTDPKPTWREYVN
                     QHTRWNAGAIYSADFVTRASYWFVVVFYLTGSLLVLPLGFLDWRVPLLSLTSFVSIGL
                     LGVIAGFYEGRARIGYFLRFVPYLVFFAFFYTFVTLRAAIRRPFEWKGARLAG"
     gene            3104..3991
                     /locus_tag="VFH83_14615"
     CDS             3104..3991
                     /locus_tag="VFH83_14615"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET6487657.1"
                     /translation="MVVLGKQWKKGEVLLADGAWGTEFLKRGLMQGCPPDEWNLSHPD
                     IVREITSEYLEAGADVILTNTFGANRFRLEPHGLSDRVAEVNRAGAAHAREVASGKAI
                     IAGDIGPTAQFLALGETSPDDLYAAFAEQARALAEAGVDWIVVESMTDAKEMALAVKA
                     SAQTTGLPVVASMTYNKTPRGYRTMMGDTPQDCVRAAQEAGAAVIGANCGSGIEDYVP
                     LAPILRGLTGLPLWVKANAGMPTITAGKVTYPLTAEQYAAFVPGLVAAGVDVIGGCCG
                     TDPRFIREIARKIPGRTGK"
     gene            3994..>4283
                     /locus_tag="VFH83_14620"
     CDS             3994..>4283
                     /locus_tag="VFH83_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487658.1"
                     /translation="MSLLVILCVLFVFFRAEILLRVATLGGPRRVMAEVSRHQHVVSR
                     KIFLLARLFSGMRVTMEGLSRALPPVFLVVSNHQSLIDIPALATAFPCRNLR"
BASE COUNT          766 a         1403 c         1417 g          697 t
ORIGIN      
        1 ggatggactt catgatcgcc cttggcagcc atacgcccat gagcgatccc gccatcgacg
       61 cccggttcgg tctgagcccg ggagaccggg agaagctgtt cccgggcagc aggttcctca
      121 accatcggtg ggaccttccc gacacgctgg agaagatcgg aacgatctcc gcccgagagg
      181 ttcgggacct caccaagggg ctcatggatg agagcgcgga tgtggtgatc aaccgcgcca
      241 tccgggagta cgacctgatt ctgattctgg ggccggtctt cccgcacgag gtgattggct
      301 tctccggcgg aaacaagtac ctgttccccg gcatctccgg cggcgagttc ctgagcttct
      361 tccattggat gggcgcgctg atcacctgcc ccgagatcat cgggttcaag aacaccgtag
      421 tgcgggcgac cgtggaccgc gccgcagcca tggtcagcgt caaccggtgg tgcctggccg
      481 gggttgtcgg tcccggtggg aagctcgccg ggctcttcgc ggggacgccc gaggaggcgt
      541 ggtcccaggc cgccgacctg tccgcgcaga tccacgtggt ctacaaggag cggccttacc
      601 ggaccgtgct cggcattgcc cccaccatgt acgacgagct ctgggtggcg ggcaaggtga
      661 tgtacaagct ggaacccgtc gtggcagagg gtggcacgct catcatccac ggcccgcacg
      721 tcaaggaggt ctcccgaacc tggggcagcc acctccttcg gaccgggtac cacgtgcgag
      781 actggttcct ggcccaccct gagctgggta cgggtgtgcc cagggcggtc ctcgcccact
      841 ccacccacgt acgcgggatt ggatcggtcg tggcgggggt ggagaagccg agaatccagg
      901 tggttctggc aaccggcatc ccggagcagg tctgccggca gatcggtctg gggtactgcg
      961 acccgggatc gatccatccg tccgagttcg ccggccggga ggacgagggc gttctcctgg
     1021 tggatcacgc gggagaggtt ctccaccgac tggagcagga ccgggccagg atgaaatcat
     1081 gaacgggcac accctggtgg ttctggattt cgacggcgtg atctgcgaca ccatcgacga
     1141 gtgcttcgtc tcctcctgga tcgcctacca ttccctgtac cacaacgagg cgctgcggcc
     1201 gccggcccgc accatccgca acgagttcgc caggcttcga ccgttcgtgc gaagcggaga
     1261 agacttcgtg cgcatccagg agatcctggc tacgggagcc agagtcacgg accaggcggg
     1321 gttcgacgag ctcacgcggc aggctggcga ggaaagccgc aaacgctttc gggaggtctt
     1381 ctaccaggcc cggacggaga tgctggagcg ggaccgcgac gggtggctgg ccctcagccg
     1441 catctaccca catgtgatcc tggcttttgc ccacctcccc cgggaggttc ccctgtacgt
     1501 gctctcgacg aagaagcggg agttcgtcga agagatcgtt cgcgcggagg gcatccagat
     1561 cccgtccgag aagatcctgt ggagcgaccg ggagcccaag ctggcaacgg tccaccgcct
     1621 cctcgaggaa ggccgctacc ctcaagccat cctcgtcgac gaccagatcg actacctggc
     1681 caccaacgcg gaccctcgca tccgcgtcta tctggcttcc tggggctacg tgcaaaagga
     1741 atggctgtgc gagcccttga aggttccggt cctggctccc caggacctta agcctctgct
     1801 cgaggaagcg ctggctcccg cccgcaccgc gtcgtagcgc gccggccggc ccccgcgcct
     1861 catcgtgccg cccctagccg gccaaccgcg ctcccttcca ctcgaacgga cggcggatgg
     1921 cggcccgaag cgtcacgaag gtgtagaaga acgcaaagaa cacgaggtag ggaacgaaac
     1981 gcaggaagta gccgatgcgg gccctcccct cgtagaatcc cgcgatcact cccaggagtc
     2041 cgatgctcac aaacgatgtg agcgacagga gcggtacgcg ccagtccagg aatccgaggg
     2101 gcaggaccag caggctcccc gtcaggtaga agacgaccac gaaccagtag ctggcgcgcg
     2161 tcacgaagtc agccgaatag atggccccgg cgttccagcg cgtgtgctgg ttgacgtact
     2221 cccgccaggt gggcttgggg tccgtcagca tggccgcgcc ggaggctgtg cacacggcgc
     2281 ctttccatcc cgcgccgcgg cagacagcgt ccagcagcat tgcgtcctcc gtcacggaaa
     2341 acccgagcgc ctgaaaccca ccggtctgtc ggaccgcgac ggcgcggacg gccatgttgt
     2401 tgccgaaacc gcccgtaggc agccccacgc ccagggaacc gaagttgtat tgattgagca
     2461 gcggctgctc gaagccctgg aatcggagca ggaatccctt tctcgactcg agctcgatcc
     2521 tcccgaggac cacgcccgtg cgcgggtcgg cgaagtgccg catcatctct tccacccacg
     2581 tagggggcgg cgagcagtcg ccgtccgtga agagaagcac gtctccgcgc gcctgggaga
     2641 atgcgaggtc cagggcggcc tgtttgccgg tgaggccctc cggctcccgt tcggcgcgca
     2701 gcaggcgcgc gcgcgacccc aggtcccggc agaatcgctc cagaagctgc ggcgtggcgt
     2761 ccgtggaccg gtcatccacg aggaggaaca ggcagtcccg gcgggtctgg gcaccgagcg
     2821 ccgacaacag ccgcggcagc aacttttcct cgtttcggac cgccacgatc acttctgcga
     2881 tcagagctcc ggtcggtccg cccgcgcctg aggacgcccc cacccttgcc agccgccgct
     2941 cgcgggccag attcagcagg catccgaggg ccaggatgga atggaagcag agaatgacgg
     3001 cgcttatcgc ggcaaagacg agtagaaatg cctccatgct cgaaactagc ctaatggaaa
     3061 gccatgcttt ccacaagcgc gcggaaaaag tagaataagg gtaatggtgg tcctcggtaa
     3121 gcaatggaaa aagggtgaag tcctcctggc ggatggagcc tgggggacgg agttcctgaa
     3181 gcggggattg atgcagggct gccccccgga tgagtggaac ctctcccatc ccgacatcgt
     3241 tcgagagatc acatcggagt acctggaggc cggagcggac gtcatcctga cgaacacctt
     3301 tggcgccaac cggtttcggc tggagccgca tgggctctcg gaccgggtgg cggaagtgaa
     3361 ccgcgccggt gcggcgcatg cccgggaggt ggcctctggc aaggccatca ttgccgggga
     3421 catcgggccc acggcccagt tcctggccct gggggagacc agtccggacg acctgtatgc
     3481 cgccttcgcg gagcaggccc gtgcgctcgc ggaagcaggt gtggactgga ttgtcgtcga
     3541 atcgatgacg gacgccaagg agatggcgct ggccgtcaag gcgtccgccc agaccacggg
     3601 tctgccggtg gtcgcgagca tgacgtacaa caagacgccg cgcggctacc gcaccatgat
     3661 gggagacacc ccccaggact gcgtccgggc tgcccaggaa gccggcgccg cggtgatcgg
     3721 tgccaactgc ggatccggca tcgaggacta cgtccccctc gcgcccatcc ttcgcgggct
     3781 gacggggctg ccgctctggg tcaaggcgaa tgccggcatg ccgaccatca ccgcgggaaa
     3841 ggtcacctat ccgctcaccg ccgagcagta cgcagccttt gttccgggcc tcgtggccgc
     3901 gggggtggac gtgatcggcg gctgctgcgg gaccgacccc cggttcatcc gggagatcgc
     3961 ccggaagata cccggaagga cggggaagta gcgttgtccc tccttgtcat cctgtgcgtc
     4021 ctgttcgtct tcttccgcgc cgagatcctg ctgcgggtgg ccaccctcgg tggaccgcgg
     4081 cgcgtcatgg cagaggtgag ccgccaccag cacgtggtct cgcggaagat ctttctgctg
     4141 gccaggctct tttccggaat gagggtgacg atggagggcc tctcccgagc ccttcctccc
     4201 gtgttcctgg tggtcagcaa tcaccagagc ctgatcgaca taccggccct ggctaccgca
     4261 tttccctgcc gcaacctgcg cta
//
LOCUS       DASRZS010000602         6915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2248459, whole genome shotgun sequence.
ACCESSION   DASRZS010000602 DASRZS010000000
VERSION     DASRZS010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2248459"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VFH83_14625"
     CDS             complement(<1..827)
                     /locus_tag="VFH83_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487659.1"
                     /translation="MSGGVEPRPTPTRARSGGFVAGLRGFLTQWEIILVYLLVIVFVG
                     NSLLSPYFLDVRNLLDSTFNFTEKAIIALPMMLVIISGDIDISVASNLALCSVFMGMA
                     MTAGAPTGVLVLIGLASGSLMGWLNGFLITAFSIPSIAVTIGTLSLYRGIAAVILGDG
                     AYIQYPKPFAYFGQGYLPGTGIPFELVLFALLAAIVGFVLHRTTFGRRIYAVGNNATT
                     ARFSGVKVDRLRLVCFTLNGLAAGVASILLTSRLGSTRPNIATGWELDVITLVVLGGT
                     "
     gene            complement(824..1798)
                     /locus_tag="VFH83_14630"
     CDS             complement(824..1798)
                     /locus_tag="VFH83_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487660.1"
                     /translation="MKELVKRREVTLLGIMLVVVVVVTLRNSAFLTLRNFEDILKDSS
                     ILLIVSIGQLLVILTGGIDLSVASTIGFAGMSVALLNHLVPGIPLVVILLIAMGIGLV
                     LGSFNGTLVSVFGIPPIITTLGTLSIYRGFIIILSGGKWVSADQMTAAFREFPQDRLL
                     GLSNMISLAILVSLIFAGILYLTRTGREIHGVGGNRLAAQYVGINIRRIDFLVFALSG
                     VLAGLAGYLLVAFYASAANDSASGLELQTVAACVIGGVSILGGSGTIVGVILGSVFLG
                     IVKNALTVIRISPFWQVAIQGIVILFAIVLSTVFDRRAQAGILRRRVL"
     gene            complement(1798..3303)
                     /locus_tag="VFH83_14635"
     CDS             complement(1798..3303)
                     /locus_tag="VFH83_14635"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6487661.1"
                     /translation="MSKGFPGVKALDQVRMSVRRGEVHALIGENGAGKSTLVKILSGV
                     FPPSEGEILLEGKPVSFRSPHESQAAGVSAIHQEATMFPELSVTENIFMGHHLLRRGV
                     VQWGAMEERTRGLLQALDIKLNPRAKVKTLSFAERQMVEIVKALSINAKVVIMDEPTS
                     ALTLREVEHLYAIIRQLARSGTSIIFISHKFEEIFEIADSYTILRDGRWVDEGTVAGT
                     TVDDIVRKMVGRSLEQMYPKSDVPFGPPVLRVEGLSRNGIFKDVSFELRRGEILGFFG
                     LVGAGRSEVMRVLFGIDPCDGGEIFVDGKRVEIRSAAAAMRCGLALLPEDRQLQGVIL
                     PMSIRENTTLPIVGSLARRGVLRRSVETEVTERYGSLFQVKCAGWDVAVNSLSGGNQQ
                     KVVLAKWLATDPRVLVLDEPTKGIDVATKATVHKFISDLAAQAMGIILVSSELPEILG
                     MSDTIIVMHEGRVTARIPRAEATSEKVLLAATATEDGGSVAATPRSGGSGA"
     gene            complement(3434..4474)
                     /gene="rhaS"
                     /locus_tag="VFH83_14640"
     CDS             complement(3434..4474)
                     /gene="rhaS"
                     /locus_tag="VFH83_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6487662.1"
                     /translation="MTITKKLLVLLLACLLIGSFAFAGGQKEGAAAPSATGKITIAFV
                     PKTLGIAVFDAMDNGAKEAAKDTGANVEIVYQGPSSPTAEGQISIISTLIAQHVNAIE
                     ISTNDTDALVPICKKAMDAGIKVISYDSAIAPEGRILHVAPWDDEQVGRSAVQLISKM
                     IKNTGEIAILSTSATTTNPNTWIKWMKAELQDPKYANIKLDAIAYGYDEADKSYTEAM
                     GLFKSYPNLAGIISPTTIGIAAAAKALEDASLAGKIQLTGYGLPSQMKKYIQDGTCTA
                     MELYNPVDLGYASTFIAYDLVKGNLSSVKVGDSFDMGRMGKRTVLDGNVIVQGNTFVF
                     DKSNIDQYAAIF"
     gene            complement(4629..5417)
                     /locus_tag="VFH83_14645"
     CDS             complement(4629..5417)
                     /locus_tag="VFH83_14645"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET6487663.1"
                     /translation="MGSGAEEEKIFVEERRNRILDLLSRKNKASVAELSQEFRIGEAT
                     IRKDLNDLEQRGLILRTHGGALIMDTASKEAPLMERETRNREQKERIAQFIAQFIRNG
                     ESIMMDGGTTTLQIGRSLRARRNLVVVTNSPPLAAEMVNFVDTRVILTGGELRESTGA
                     LVGPLTTYSIRQFRVDRVILGMSSLVPDQGLFTANASEAEVKRVMMECGKEVIVAMDS
                     GKIGKLTLSFVSDFSMVGKLVTDTGISAEDLEKIERKGVEVLAV"
     gene            5681..>6915
                     /locus_tag="VFH83_14650"
     CDS             5681..>6915
                     /locus_tag="VFH83_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6487664.1"
                     /translation="MRPRRNKGFKIGFLVAIAAALVFPATGALAAPAPIKIDIYWWGD
                     QMTDANNAIAQAYARIHPEFVVNFVKTDAPAYTLQFQTWFQSNSAPVLMGGGGMDSEN
                     AIKPGFLAAWDPYLEMPDPYAGNKAWKDTLHMEWLVRSRFEEDNKIYMLPTWADQAGV
                     YYNKSIYDKLGLKVPTTWDQWMDNNKKIKAAGYDAMQIGGNVVWMQAEIMDAVWREHA
                     AEMYKDPKYPKVDLNNTFDDTRVAPRPNWSYWSYKEGTLKPDSPQTRFMAEKMLQLSQ
                     FFPKGTPQLESYGNAKLDPILLSFVNSRLASWYGAGWMLPALSALTKDLPAGKRFEVG
                     YFTLPGWGDKVIKAPGYDFMLPPLRPATGRDPSNANFVLSSKASEDDRRRAVDFIMFF
                     YSPKSYSDLIKSTAVGEFP"
BASE COUNT         1293 a         2227 c         2195 g         1200 t
ORIGIN      
        1 gtgccgccca ggacgacgag cgtgatgacg tccagctccc agccggtcgc gatgttcggt
       61 cgggtgctgc ccaggcggga ggtgagcagg atcgaggcca cgcccgcggc cagaccgttc
      121 agcgtgaagc ataccagccg cagtcgatcg accttcacgc cggagaatcg cgcggttgta
      181 gcgttgttcc cgacggcgta gatccgccgc ccgaaggtgg ttcgatggag cacgaatcca
      241 acgatggcgg cgagcagggc gaacagcacc agctcgaacg ggatcccggt gccggggaga
      301 tagccttggc caaagtaggc gaagggcttg ggatactgga tatacgcccc gtcaccgagg
      361 atgacggccg cgatcccacg gtacagcgaa agagttccga tggtgaccgc aatcgaaggg
      421 atcgagaacg ccgtgatcag aaacccgttg agccagccca tgagcgagcc ggacgccagg
      481 ccgatcagca ccagcacccc cgtgggcgcg ccggcggtca tggccatgcc catgaacacc
      541 gagcacagcg cgaggttgga cgcgacggag atatcgatgt ccccgctgat gatgaccagc
      601 atcatcggca gggcgatgat cgccttctcg gtgaagttga aggtggagtc gagcaggttg
      661 cggacgtcca ggaagtaggg cgacagaagg gagttcccga cgaagacgat gaccagcagg
      721 tagaccagga tgatctccca ctgcgtcagg aacccccgaa gccccgcgac gaagccgccc
      781 gatcgggcac gggtgggcgt cgggcgcggc tcgacccctc cgctcacagg accctcctcc
      841 gcaggatgcc cgcctgggcg cgccgatcga acaccgtgct caggacgatc gcaaagagga
      901 tcacgatgcc ctggatggcg acctgccaaa acggtgagat gcggatcacg gtaagggcat
      961 tcttcacgat cccgaggaag acggacccca ggatcactcc cacgatggtc cccgagccgc
     1021 ccaggatgct tacgccaccg atcacgcacg cggcgacggt ctgcagctcc agaccgctcg
     1081 cggagtcgtt cgcggccgag gcgtagaagg ctaccaggag gtagccggcc aacccggcca
     1141 ggacccccga aagggcgaag accaggaagt cgatcctccg gatattgatc ccgacgtact
     1201 gcgccgcaag acggttcccc cccacgccat ggatctcgcg tcccgtccgg gtgagataga
     1261 ggatgccggc gaagatcagg gagaccagaa tggcgaggga gatcatgttc gagagtccga
     1321 gcagtcggtc ctgggggaac tcgcggaacg ccgcggtcat ctggtcggcg ctcacccact
     1381 tgccgccgga caggatgatg atgaaacccc ggtagatgct cagggtgccc aacgtcgtga
     1441 tgatcggcgg gataccgaag acggacacca acgtcccgtt gaaggagccc agcaccagtc
     1501 caatgcccat ggcgatcaac aggatgacca ccagcgggat gccgggcacc aggtggttga
     1561 gcagggccac gctcatgccg gcgaagccga tcgttgaggc gaccgacagg tcgatgccgc
     1621 cggtaaggat gaccaggagc tgcccgatgc tcacgatgag caggatcgag ctgtccttca
     1681 ggatatcctc gaagttgcgc agcgtgagga aggcgctgtt gcgcagcgtc acgaccacga
     1741 cgaccaccag catgatgccg agaagggtga cttcccgacg cttgaccagc tccttcatca
     1801 cgcgcccgat ccacccgagc gtggggtcgc agccaccgat ccgccatctt cggtcgcggt
     1861 ggccgccagc agcaccttct ccgaagtggc ttccgcccgc gggatccgcg cggtgacgcg
     1921 gccttcgtgc atgacgatga tggtgtcgga catgccgagg atctccggca gctcggagga
     1981 gaccaggatg atccccatgg cctgggccgc caggtccgag atgaacttgt ggacggtggc
     2041 cttcgtcgcc acgtcgatgc ccttggtcgg ctcgtccagc acgagcacgc gcggatccgt
     2101 ggccagccac ttggccagga ccaccttctg ctggttgccg cccgagaggc tgttcaccgc
     2161 cacgtcccat ccggcgcatt tcacctggaa gagcgaaccg tatcgctccg tgacctccgt
     2221 ctccacggac cgccgaagga cgcctcgacg cgcaagggaa ccgacgatgg gaagcgtcgt
     2281 gttctcgcgg atgctcatcg gcaggatcac gccctgaagc tggcgatcct cgggaaggag
     2341 ggccagcccg cagcgcatcg ccgcggcggc gcttcgaatc tccacgcgct tcccgtcgac
     2401 gaagatctcc ccgccgtcgc acggatcgat gccgaacagc acgcgcatca cctccgagcg
     2461 cccggcgcct accaggccga agaaccccag gatctctccc cgccgaagct cgaaggatac
     2521 gtccttgaag atcccgttcc gcgagaggcc ttccacccgg agcaccggcg ggccaaacgg
     2581 gacatcgctc ttggggtaca tctgctccag gctgcggccg accatcttgc gcacgatgtc
     2641 gtccaccgtg gtcccggcca cggtgccctc atccacccat cggccgtcgc gcaggatggt
     2701 gtaggagtcc gcgatctcga agatctcctc gaacttgtgg gagatgaaga tgatcgacgt
     2761 gccgctgcgg gcgagctgtc ggatgatcgc gtagagatgc tcgacttccc gcagggtcag
     2821 cgccgaggtc ggctcgtcca tgatgacgac cttggcgttg atggagagcg ccttgacgat
     2881 ctccaccatc tggcgttcgg cgaagctcaa cgtcttgacc ttcgcgcgcg ggttcagctt
     2941 gatgtccagc gcctggagga gcccgcgggt gcgttcctcc atggctcccc actggacgac
     3001 gccgcggcgc agcaggtggt ggcccatgaa gatgttctcc gtcacggaga gctcggggaa
     3061 catcgtggcc tcctggtgga tggccgagac cccggccgcc tgggattcgt gcggcgagcg
     3121 gaaggagacc ggcttcccct ccaggaggat ctcgccctcg ctgggcggaa agaccccgga
     3181 gaggatcttc accagcgtcg atttgcctgc gccgttctcc ccgatgagcg cgtggacttc
     3241 gccgcggcga acgctcatgc gaacctggtc gagcgccttg acgccgggga aacccttgct
     3301 cacaccgctc agccgcagga tctcttcggc ggcagtctgc gttggttggt ccgtaggcgt
     3361 tgtgtcctct ggttaccgag ctcgagacgg ctgcggccgc gcgggcgcaa ccgtctcgag
     3421 agctccaagc ggcctagaag atcgccgcgt actggtcgat gttgctcttg tcgaacacga
     3481 aggtattgcc ctggacaatg acgttgccgt ccaggacggt gcgcttgccc atgcggccca
     3541 tgtcgaacga gtcgccgacc ttcacggagg aaaggttccc cttcaccagg tcataggcga
     3601 taaaggtgga tgcgtacccc aggtccaccg ggttgtacag ctccatggcg gtgcaggtgc
     3661 cgtcctggat gtacttcttc atctgcgacg gaagcccgta gccggtgagc tggatcttcc
     3721 ccgcgaggct cgcgtcctcc agcgcctttg cggccgccgc aatcccgatc gtggtcgggg
     3781 agatgatgcc cgccaggttc gggtaggact tgaacaggcc catggcctcc gtgtagctct
     3841 tgtccgcctc gtcgtagccg tacgcgatgg cgtccagctt gatgttggcg tacttcggat
     3901 cctggagctc ggccttcatc cacttgatcc aggtgttcgg gttggtggtc gttgcgctgg
     3961 tgctcaggat ggcgatctcg cccgtgttct tgatcatctt gctgatgagc tggaccgcgg
     4021 agcggccaac ctgctcatca tcccagggcg ccacgtgcag gatgcgtccc tcgggagcga
     4081 tggcggaatc gtaggagatg accttgatcc cggcgtccat cgccttcttg cagatgggga
     4141 ccagcgcgtc cgtgtcgttc gtggagatct cgatggcgtt cacgtgctgc gcgatcagcg
     4201 tggagatgat ggagatctgt ccttccgcgg tcggggaaga gggaccctga taaacgatct
     4261 cgacgttcgc gccggtatcc ttggccgctt ccttggcgcc gttgtccatg gcgtcgaaca
     4321 cggcgatgcc gagcgtcttc gggacaaagg cgatggtgat cttcccggtg gccgacgggg
     4381 ccgcggcgcc ctctttctgg ccgcctgcga atgcgaacga gccgatgagc agacacgcca
     4441 gcaacagtac gagaagcttc ttcgtaatgg tcacagcgtc ctccttggga gattgggctc
     4501 agcgatcgca cggtcgcttg gatggactct atcactggtc aatgagggcg tcaaggaaat
     4561 gtccagagga tccgcaggat gcgccgccgg cggcgcggat ccaggatcgc gaagaccgcc
     4621 gtgccgcttc agacggccag gacctccacc cccttgcgct cgatcttctc gagatcctcc
     4681 gccgagattc cggtgtccgt gacgagcttc cccaccatgg agaagtcgga cacgaagctc
     4741 agggtcagct tgccgatctt gccggagtcc atcgccacga tcacctcttt cccgcattcc
     4801 atcatcacgc gcttcacctc cgcctcggag gcgttcgccg tgaagaggcc ctggtccggc
     4861 acgagggagg acatccccag gatcacccgg tccacgcgga actggcggat cgagtacgtg
     4921 gtgagcgggc ccaccagcgc cccggtggat tcccgaagct caccccccgt gaggatcacc
     4981 cgggtgtcca cgaagttcac catctcggcg gccaggggcg gggagttcgt gacaacgacg
     5041 aggttcctcc gcgccctcaa cgatcgaccg atctgcagcg tggtggtgcc cccgtccatc
     5101 atgatgctct caccgttgcg gatgaactgc gcgatgaact gggcaatccg ctccttctgc
     5161 tcccggttgc gagtctcccg ctccatcagg ggcgcttcct tggaggcggt gtccatgatc
     5221 aaggcgccgc cgtgcgtcct caggatcagc ccccgctgct ccagatcgtt caggtccttg
     5281 cggatggtgg cctcgccgat ccgaaactcc tgcgaaagct ccgcaacgct tgccttgttc
     5341 ttccgggaga gaagatcgag gatgcgattg cgcctctcct cgacgaagat cttctcttct
     5401 tcggcgccgc tacccactat gccctcctgc cgcaatcggg atagtacgaa tcgccgggct
     5461 ttgcaatagg aatcggaatg acggtttctg acgttcgagg cccgccaggc cgataccacc
     5521 accgaccgat tccatagcgc atcaaatgaa agctcgtttt tcgttcgatt ggtgcatctt
     5581 tccttgacaa cagcatcccg gtgcggtacc gtccgctcac acgggtctgg agtggagcgg
     5641 cctacggcgc gcggcccgga cccgatcaag gaggtggacg atgcgtccaa gacggaacaa
     5701 ggggttcaag atcggattcc tcgtcgctat cgccgcggcc ctggtctttc cggcaaccgg
     5761 cgccctggcg gctccggcac cgatcaagat cgacatctac tggtggggag accagatgac
     5821 ggatgccaac aacgccatcg cccaggcgta tgccaggatc catccggagt tcgtggtcaa
     5881 cttcgtcaag acggacgccc ccgcctacac gctccagttc cagacctggt tccagagcaa
     5941 ctcggcgccc gtcctgatgg gcggcggcgg gatggacagc gagaacgcga taaagccagg
     6001 cttcctggcc gcgtgggatc cgtacctgga gatgccggac ccgtacgccg ggaacaaggc
     6061 gtggaaggac acgctccaca tggagtggct ggtccggtcc cggttcgaag aggacaacaa
     6121 gatctacatg ctgcccacgt gggcggacca ggccggtgtc tactacaaca agagcatcta
     6181 cgacaagctc ggcctcaagg tgcccaccac ctgggaccag tggatggaca acaacaagaa
     6241 gatcaaggcc gccggctacg atgccatgca gatcggcggg aacgtggtat ggatgcaggc
     6301 cgagatcatg gacgccgtct ggcgcgagca cgcggcggag atgtacaagg accccaagta
     6361 ccccaaggtg gacctgaaca acacgttcga tgacacccgg gttgcgcctc gccccaactg
     6421 gagctactgg tcctacaagg aagggaccct gaagccggac agcccccaga cccggttcat
     6481 ggcggagaag atgctccagc tctcccagtt cttccccaaa ggcacgcctc agctcgaatc
     6541 ctacgggaac gccaagctgg atcccatcct gctgagcttt gtgaacagtc gcttggcctc
     6601 ctggtacggc gcgggatgga tgctccccgc gctgagcgcg ctgaccaagg atctgcctgc
     6661 cggcaagcgc ttcgaggtgg gctacttcac gctgccggga tggggggaca aggtgatcaa
     6721 ggcccccggc tacgacttca tgctgccgcc gcttcgtccg gccacgggaa gagaccccag
     6781 caacgccaac ttcgttctct cctccaaggc gtcggaggac gaccgccgtc gcgcggtgga
     6841 cttcatcatg ttcttctatt cccccaagtc ctactcggac ctgatcaaga gcacggccgt
     6901 gggggagttc cccat
//
LOCUS       DASRZS010000603         6492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2657282, whole genome shotgun sequence.
ACCESSION   DASRZS010000603 DASRZS010000000
VERSION     DASRZS010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2657282"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1689)
                     /gene="cydC"
                     /locus_tag="VFH83_14655"
     CDS             complement(<1..1689)
                     /gene="cydC"
                     /locus_tag="VFH83_14655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HET6487665.1"
                     /translation="MRRLASLVLPHWRMALLGIFLSLLALLANMTLLALSSWFIASMA
                     LAGSLGATMDYTTPAAAIRGLALARAGGRYAERLANHATTLRILGSLRVWFFQRIEPL
                     APARLASHRGGDLLARIRSDVDSLDDFYVRGLVPTIAAGLAVLCLAPFLARFDARVVA
                     VDLGGLCLGGVLLPLLLRTLADEPGRGRVADAGLLRALVVEQAQGMAELVALGAIEEH
                     TERIVHASRSLDARQRRLGSLQGVGEAGLVAASSLATWATAALVMGDLARGALPPVDL
                     AVLTVLVLASFEIVMPLPGVIQRFGEMKAAAQRLFDLIDTPPAVVDQGDRSWPASNAP
                     VGVAIHGLRFRYDAAAPLVFDGLAMDVEPGVCTAIAGPSGCGKSTLVSLLLRFWEYEA
                     GSVTIRWEGASAELRELAGDDARRLFSVLPQSPYLFHASIRENLLLASQPDREPGDGD
                     LLEALRAAELGALMAALPDGLDTTVGEQGRELSAGEARRVALARMFLRDAPIYVLDEP
                     TEGLDPATADAVLFTLRRRLRGRTVILMSHRDRDLSIADRVVRLPGPPIPGAPGP"
     gene            complement(1686..3371)
                     /gene="cydD"
                     /locus_tag="VFH83_14660"
     CDS             complement(1686..3371)
                     /gene="cydD"
                     /locus_tag="VFH83_14660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HET6487666.1"
                     /translation="MRGDSEAAAGGARWLRDQGRPARGRLALTVALGEAAGVLLVLQT
                     GLLARVADGAIFRRGSLPALLPLLLEALIVIGVRAVTTWASKRAASACASQAKDLIRS
                     RCLEQLRRLGPIPLAGMRAGEIANTAVDAVEALDAYYSRYLPQRAIASLLPLTILAVI
                     FPLDWLSGLVLVLTAVFLPLSMIVIGEEAHERNRRLWGTLAQMSGRFLDVLQGLATVR
                     MFNAAARETRQIQEASDQYRALTLSVLRVAFLSSFMLELISAVSIAIVAVLTGFRLLS
                     GSMSFLPGYFILLIAPEYFLTLRVLGTFYHSRMEAVSAAEKIRELLSLPVPDRAHAPA
                     VAVRLSAAPSVGFQDVTFSYEGRSVLSGASFRIPPGEHVALMGESGAGKTTVLLLLLG
                     FAAPRSGTIDVDGQPLGALDADGWRAGVAWLPQRPTLFHGSVLDNIRLGSANATRDEV
                     LAAARLAHVDEFLPRLSHGLDTRLGEGGQGLSVGQAQRVALARLFLRQPRVVLLDEPT
                     AHLDDESARLVTEGIQRLCAGRTTLLVTHREATAAFMGRVIRISDGRIREAPP"
     gene            3495..4802
                     /locus_tag="VFH83_14665"
     CDS             3495..4802
                     /locus_tag="VFH83_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET6487667.1"
                     /translation="MSVAGLSQIQFATVALFHFLFVPLTLGLSVLVAIMETAYARTGN
                     EIYLRMTRYWSRLLIINFAVGVVTGITLEFQFGMNWAEYSRFVGDIFGAPLAIEATVA
                     FFLESTIMGLWLFSWKRVSRGVHATLMWLIALASTLSAVWILVANGWMQHPVGYQVVG
                     GKAELTSFVALITNHYALVKIFHTLSSAYTLSCFFIMGISAIQLLQHRHEEMFTRSFK
                     IAATIGIVTSIFIIVAGDLSGTQVALTQPTKLAAMEAQWETETGASYYILQFPDVKQG
                     ENSVQAIGIPKGLSILAYRDPGATIKGLNDFPASDRPSVMISFLSFRLMVGLGFLFVL
                     LAFIAVLIAWRRRLGKNRWFLWVMAVAIVLPYLASELGWMLAEMGRQPWIVYGLMRTA
                     DAASRSLTPGDVLGSLIAFLALYSILAVVDVFLLSRYARTVED"
     gene            4835..5845
                     /gene="cydB"
                     /locus_tag="VFH83_14670"
     CDS             4835..5845
                     /gene="cydB"
                     /locus_tag="VFH83_14670"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HET6487668.1"
                     /translation="MQILWFVLWGTLWAVYFMLDGFDFGAGMLRVAIARNESDRRTTL
                     AAIGPVWNGNEVWLITAGGATFAAFPAVYASMFSFLYLPMLLILFSLILRGIAVEFRS
                     KTDNPRWRGAFDWILAITSLTAPLVLGLGFGNIFQGLLFDSSGYHGSFGGLFNPFGLL
                     TAALFVLLFLQHGALWLSYRTEGELAERSRAVAGPLWIVILVVAAAFLVAAGFATRFL
                     DNYLSRPYWFLVPAAAVAALVGVRVLQARPGRLAAFLCSCATIVLVVATGLVGLFPSL
                     IPSSLDPSASLTIYNSSSGPYTLRVMTIVALIFVPIVIVYQLLVYRFFRGKADAEGAE
                     GY"
     gene            5930..>6492
                     /locus_tag="VFH83_14675"
     CDS             5930..>6492
                     /locus_tag="VFH83_14675"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6487669.1"
                     /translation="MDIKTQSITYGPGGIYSGFAAYPERHDGSLPGVIVFQEVYGVDG
                     HIRDVTRRYAEAGYFAFAPDLYAQYGNRLAPLAEPRIEAVKRFMDKVPFAEMRDPAKR
                     DSHLSRLPPQEAKEVGESLQALFGGMDLPKRVPHLVATSAFLREENSLTRGQRIGSVG
                     FCMGGGLSGLLAASDPALSAAIIYYGRS"
BASE COUNT         1048 a         2229 c         2166 g         1049 t
ORIGIN      
        1 gggcccaggg gcccccggga tcggcggccc cggcagcctc accacccggt cggcgatcga
       61 aaggtcgcga tcccgatggc tcatgaggat gacggtgcgg ccgcgaagcc ggcgcctcag
      121 cgtgaagagc accgcgtcag cggtagccgg gtccagacct tcggtgggct cgtccagaac
      181 gtagatgggt gcgtctcgaa ggaacatgcg ggccagcgcc acgcgccggg cttccccggc
      241 ggagagctct cggccctgct cgccgaccgt ggtgtccagc ccatcgggca gggccgccat
      301 cagcgcgccg agctccgccg cacgcagggc ctccagaaga tccccgtccc ccggctcccg
      361 gtccggctgc gaagcaagga gcaggttctc ccggatcgac gcgtggaaga ggtacggaga
      421 ctgcggcagc acggagaaga gtcggcgcgc gtcgtcccct gccagctctc gcagctccgc
      481 ggaagccccc tcccagcgga tcgtcacgct gcctgcctcg tactcccaga agcgcaggag
      541 caggctgacc agggtgctct tgccgcaccc ggaaggcccg gcaatcgccg tgcaaacgcc
      601 gggctccacg tccatcgcca gtccgtcgaa caccaggggc gcggcggcgt cgtaccggaa
      661 ccgaagcccg tgaatggcca ccccaaccgg ggcgttcgac gcgggccagg accgatctcc
      721 ctgatcgaca acggcgggag gggtgtcgat cagatcgaac aggcgttggg ctgccgcctt
      781 catctcgccg aaacgctgaa tgacccccgg cagaggcatc acgatctcga agctcgccag
      841 cacgagcacc gtgagcacgg caaggtccac gggaggaagc gcgccgcgag cgaggtcacc
      901 catgacgagc gcggccgttg cccaggtggc cagggagctc gccgccacca gcccagcctc
      961 acccaccccc tggagggagc ccagcctccg ctggcgagca tccagggacc ggcttgcatg
     1021 gacgatccgc tccgtatgct cctcgatggc gcccagggcc acgagctcgg ccatcccttg
     1081 cgcctgctcg acgacgaggg cgcgcagcag gccggcatcg gcgacgcggc cgcgcccggg
     1141 ctcatcggcg agggtgcgca gcaggagcgg cagcaacacg cccccaaggc agagcccgcc
     1201 gaggtccacc gccacgaccc gcgcgtcgaa cctcgccagg aacggggcga ggcagaggac
     1261 cgcaagaccc gccgcgatcg tgggcacgag gccgcggacg tagaagtcat ccaggctgtc
     1321 cacgtccgat cggatccgag ccaggaggtc tccgccgcga tgggaggcca ggcgggccgg
     1381 cgccagcggc tcgatccgtt ggaagaacca cacgcgcagc gagccgagga tccgcagcgt
     1441 ggtggcgtgg ttggccagcc gctcggcata ccgtccgccg gcccgcgcca gcgccaggcc
     1501 gcgtatggcg gcggcggggg tggtgtagtc catggtggcg ccgagcgagc cggccagggc
     1561 catcgaggcg atgaaccagc tcgacagcgc gagcagcgtc atgttggcca gcagggcgag
     1621 aagggacagg aagatcccga ggagagccat gcgccagtgg ggaagtacga gggaggcgag
     1681 ccgcctcatg ggggagcctc ccggatccgg ccatccgaga tccggatcac acgccccatg
     1741 aaggcggccg tcgcctcccg gtgcgtgacc aggagcgtgg tgcggcctgc gcacaatctc
     1801 tggatccctt ccgtgaccag tcgcgcgctt tcgtcatcca ggtgcgcggt cggctcgtcc
     1861 agcagcacca cgcgcggctg gcggaggaac agacgagcca gggctacccg ctgcgcctgg
     1921 ccgacggaca gtccctgccc gccctcaccg aggcgggtgt cgagcccgtg cgagagccgc
     1981 ggcaggaact catccacgtg cgccagccgc gcggccgcca ggacttcgtc ccgcgtagcg
     2041 ttcgcgctgc cgagccggat gttgtccagg acggagccgt ggaacagggt cgggcgctgc
     2101 gggagccagg ccacacccgc gcgccatcca tcggcatcga gcgctcccag gggctgtcca
     2161 tccacgtcaa tcgtcccgct ccttggagcc gcgaagccga ggagcaggag caagacggtg
     2221 gtcttcccgg cgccgctttc ccccatgagg gcgacgtgct ccccaggcgg gatcctgaag
     2281 ctagccccgg agaggacgga ccgtccctcg taggagaagg tgacatcctg gaagcccacc
     2341 gacggcgcgg cggagagcct gaccgccacg gctggcgcgt gggcccgatc cggcacgggg
     2401 aggctcagga gctcgcggat cttctccgcc gcgctcaccg cctccatgcg agagtggtag
     2461 aaggtcccca ggacgcgcag cgtgaggaag tactccggcg cgatcagcaa gatgaagtac
     2521 ccgggcagga agctcatgga gccggacagg agacggaatc ccgtgagcac ggcgacgatg
     2581 gcgatgctca ccgcggagat gagctccagc atgaaggaag agagaaaggc cacccgcaga
     2641 acggacaggg tgagcgcgcg gtactggtcg gaggcttcct ggatctgccg ggtttcccgc
     2701 gccgcggcgt tgaacatccg tacggtggcc aggccctgaa ggacatccag gaaccgcccg
     2761 ctcatctgcg ccagcgttcc ccagaggcga cggttccgct cgtgcgcctc ctccccgatc
     2821 acgatcatgc tgagcggcag gaacacggcc gtcaggacga ggaccagacc ggaaagccaa
     2881 tccaggggaa agatcacggc gaggatggtc aacgggagca aggacgcgat ggccctctgc
     2941 ggcaggtagc gggagtagta cgcatcgagg gcctcgacgg cgtccacggc ggtgttggcg
     3001 atctctcccg cgcgcatccc cgccaagggg atgggaccga gccgtcgcaa ctgctccagg
     3061 caccgggatc gtatcaggtc ctttgcctgc gaggcgcagg cggaggcggc ccgcttgctg
     3121 gcccacgtgg tcacggcgcg gacgccgatc acgatgagcg cctcgaggag cagcggcagc
     3181 agcgcgggaa gggatccccg gcggaagatc gcgccgtccg ccaccctggc caggagaccg
     3241 gtctgcagta ccaggaggac gccggctgcc tctcccaacg ccaccgtcaa ggcaagccgg
     3301 ccgcgcgcgg gccgcccctg gtcccgcagc caccgggcgc cgccggccgc cgcctccgaa
     3361 tcaccgcgca tcggatcgga ctctacacgg gaagccacgg cgccgcaacg aacccctcgg
     3421 gatgcggcag cgcggaccgt tgcgcactcc ccgcaaaacc gctatggtcg aggcgcgtat
     3481 tcaaaggggg ctccatgagt gtagcgggac tgtcccagat ccagtttgct accgtagcgc
     3541 tctttcactt cctcttcgta cccctgaccc tggggctttc ggtgctggtc gccatcatgg
     3601 agacggcgta cgcccgcacg gggaacgaga tctacctccg aatgacgcgc tactggagcc
     3661 gtctgctcat catcaacttc gccgtgggtg tcgtcaccgg gatcacgctg gagttccagt
     3721 tcgggatgaa ctgggcggaa tactcccggt tcgttgggga catcttcggc gcccccctgg
     3781 ccatcgaggc aaccgtggcg ttcttcctgg aatccacgat catggggctg tggctcttca
     3841 gttggaagcg cgtctcccgc ggcgtgcacg ccacgctcat gtggctgatc gccctggcaa
     3901 gcacgctttc cgcggtctgg atccttgtgg ccaacgggtg gatgcagcac ccggtgggct
     3961 accaggtggt tgggggaaag gccgagctca cctccttcgt ggcgctgatc accaaccact
     4021 atgccctggt gaagatcttc cacaccctct cctccgccta cacgctgtcc tgcttcttca
     4081 tcatggggat ctccgccatc cagctcctcc agcatcgtca cgaggagatg ttcaccaggt
     4141 ccttcaagat cgcggcgacc atcggcatcg tgacgtccat cttcatcatc gttgcgggcg
     4201 atctctctgg cacgcaggtt gccctgaccc aaccgaccaa gctggctgcc atggaagcgc
     4261 agtgggagac cgagacggga gcgagctact acatcctgca gttcccggac gtgaagcagg
     4321 gagagaactc cgtgcaggcc atcgggattc caaagggtct gagcatcctg gcctatcggg
     4381 atcccggcgc cacgataaag gggctgaacg atttcccggc ctcggaccgc ccatcggtga
     4441 tgatcagctt cctgagcttc cgcctgatgg tgggcctggg attcctgttc gtccttctgg
     4501 ccttcatcgc ggtgctgatt gcctggcgcc gccgtctggg gaaaaaccga tggttcctct
     4561 gggtcatggc cgtggccatc gtccttccct acctggccag cgagctcggc tggatgctgg
     4621 ctgaaatggg ccgccagccc tggatcgtct acggtctcat gaggaccgcg gatgccgcct
     4681 cccgctcgct gactccggga gacgtgctgg gctcgctcat cgccttcctg gccctgtatt
     4741 cgatccttgc ggttgtggac gtcttcctct tgtcgcgcta cgcccgaacg gtggaggact
     4801 gagtcgcacg ggattccgtg cgagggagga ttccgtgcag attctctggt tcgtgctgtg
     4861 gggaaccctg tgggccgtct acttcatgct ggacgggttc gactttggcg cgggcatgct
     4921 tcgcgtggcg atagcccgaa acgaatcgga ccgccgcacg acccttgccg ccatcggtcc
     4981 ggtctggaac gggaacgagg tatggctgat cacggccggc ggagccacgt tcgccgcgtt
     5041 ccccgccgtc tacgcctcga tgttcagctt cctctacctg ccgatgctcc tcatcctctt
     5101 ctccctgatc ctgcgcggta tcgcggtgga gttccgttcc aagacggaca accctcggtg
     5161 gcgaggcgcc ttcgactgga tcctggccat aacgagcctg acggctcctc tggtcctggg
     5221 gctcgggttc ggcaacatct tccaggggct gctcttcgat tcctccggat accacggctc
     5281 gttcggcgga ctgttcaacc ccttcgggct cctcaccgcg gcgctcttcg tgctcctctt
     5341 cctccagcac ggcgcgcttt ggctgtccta ccggaccgag ggtgagctgg cggagcgcag
     5401 ccgggccgtt gccggtcccc tgtggatcgt gatcctggtg gtggcggcgg cgttcctcgt
     5461 ggcagcgggg ttcgccaccc ggttcctgga caactacctc tcgaggccgt actggttcct
     5521 ggtccccgcg gcggcggtcg ctgcgctggt cggcgttcgg gtccttcagg cgcgccctgg
     5581 ccggttggcg gcgttcctct gctcctgcgc tacgatcgtg ctcgtggtgg cgaccgggct
     5641 ggtggggctc ttcccgagcc tgattccttc cagcctggat ccatcggcca gcctcaccat
     5701 ctacaactcc tcctcaggtc cctataccct gcgggtgatg acgatcgtgg cgctcatctt
     5761 cgtgcccatc gtgatcgtct accagctcct ggtctatcgg ttcttccggg gcaaggcaga
     5821 cgccgaaggg gcggaggggt actgaggcag gcagcggcag ctgcgacagg cggcgcctcc
     5881 gcgtttcgat cccccaccgg gcactagtat attggcccag ggggtttgga tggacatcaa
     5941 gacacagtcg atcacctacg gtccgggcgg aatctactcc ggattcgctg cctaccccga
     6001 gaggcacgat ggctcccttc ccggcgtgat cgtctttcag gaggtctacg gggtcgacgg
     6061 tcacatccgg gacgtgacgc gccggtacgc ggaggccggc tatttcgcct ttgcgccgga
     6121 cctctacgct cagtatggca accggttggc cccgctcgcg gagccgcgca tcgaggccgt
     6181 gaagcggttc atggacaagg tgcccttcgc ggagatgcgg gatcccgcca agcgcgactc
     6241 ccacctctcc aggctgcctc cccaggaggc aaaggaggtg ggcgagtccc tccaggccct
     6301 cttcggcgga atggacctcc ccaagcgcgt ccctcacctc gtggccacca gcgccttcct
     6361 tcgggaagag aacagcctca cccgcggcca gcggatcgga tccgtggggt tctgcatggg
     6421 cggaggcctc tccggcctgc tggcggcgtc agacccggcg ctctccgccg ccatcatcta
     6481 ctacggccgc tc
//
LOCUS       DASRZS010000604         4042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5720709, whole genome shotgun sequence.
ACCESSION   DASRZS010000604 DASRZS010000000
VERSION     DASRZS010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5720709"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFH83_14680"
     CDS             <1..436
                     /locus_tag="VFH83_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HET6487670.1"
                     /translation="AAHAAASAPAPGPWIDPTHTILNPSSGLVTWVRHTFMDGIFAHL
                     NFAVFQTMVVSLEMLIGLALFGGLFTWWAAVASIGMCLLFTFTGMFAWNQAWFIFAGI
                     LMMGGAGRAFGLDSWVVPFVKRWWNGTRLARKTYLYADEPTK"
     gene            441..1409
                     /locus_tag="VFH83_14685"
     CDS             441..1409
                     /locus_tag="VFH83_14685"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET6487671.1"
                     /translation="MGSFWDGYPELERELEAVRTLILDRATTDDRDVRSAIAHLLSSS
                     GKMLRPAFVLLASRFGTPDPGRTRRVAAAVEMLHMATLVHDDIIDGASRRRGVPTLNA
                     TAGARTAVLVGDWLLASSVSLIVDLGETQDARALAAITARICAGEISQSTDRGVVHTS
                     VRRYLRRIAAKTAALFSLSFYVGARESGCGEPVSSVLRRLGYCLGMGFQIIDDILDFH
                     REGARTGKPTGNDLAQGIYTLPVILALRTADGPRLRAVLRRPPRSSHSAARAAGLVRD
                     VGGIDAARRVAAAYTERSLREISRLPATDARRTLEVVTARLLHRDH"
     gene            1506..2075
                     /locus_tag="VFH83_14690"
     CDS             1506..2075
                     /locus_tag="VFH83_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487672.1"
                     /translation="MRKTVLAACVAALLVFGTAGAFAAGEVKSTTFPWMASFDQPGQL
                     NLNVSAGFYGLGIDVNVGPEFIIGKFDIAGIPLSWGATVRGLVGFSSFFGVSWIDWAA
                     APMVTLHWGVDFGAPWKFDWYVGAGLSISGTTGTYYSTLGLAQGAVGFGFASADGVAW
                     HFSNNIALLAEYAYTPYVSTAGVGVKFAL"
     gene            2145..2279
                     /locus_tag="VFH83_14695"
     CDS             2145..2279
                     /locus_tag="VFH83_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487673.1"
                     /translation="MNPTLAKIVILGIPLLLSLSWFVYWVLRITFHQKRQKLLGRNRA
                     "
     gene            complement(2303..3505)
                     /locus_tag="VFH83_14700"
     CDS             complement(2303..3505)
                     /locus_tag="VFH83_14700"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HET6487674.1"
                     /translation="MRSLDERKKKLVGDIIRQLHQGLPVEKAKDRILEEVGRLSSAEI
                     TGIEQSLIEEGLPAEEIKRFCNVHALLFEHALEQAVATPDQPAHPVNALTRENRHIEE
                     ISARLKKALSTGDAAGARSALTELRGVDRHYAIKENAIFPFLERHGFTGPSKVMWSKH
                     DEIRALLKRAEAGAPNEMPDPGALSALTAEVDGMVFKEEKILFPAALERIDTSEWVQV
                     LKGAEEIGYPYAGTAGLHQRLEEAEALESGSAAGSGGGSVAAPTAAPGTVALPSGTLT
                     VEQLTAMLDTLPVDISFVDAEDRVRYFSQSKDRIFVRATSVLGRAVQDCHPPKSLERV
                     NRIIASFRSGARDHADFWIDLHGKRVHIRYFAVRNPAGKFLGTLEVTQDIGPLQALTG
                     ERRLEQEE"
     gene            3676..>4042
                     /locus_tag="VFH83_14705"
     CDS             3676..>4042
                     /locus_tag="VFH83_14705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6487675.1"
                     /translation="MPGRQVLDGLSLSVRRGAVAVLLGAADAGKTTVSRLIAAQIPRF
                     TGGEVSGSIELHGRDLTGAKPYELLELVGVVSQDSNEQIITARCDTEVAFALESLGLP
                     RRQIVERVDEALDLLGLSGF"
BASE COUNT          628 a         1331 c         1308 g          775 t
ORIGIN      
        1 tgccgcgcat gccgccgctt ccgccccggc gcccggaccc tggatcgatc ccacgcatac
       61 catcctcaac ccgtcgtcgg ggctggtaac gtgggtccgc cacacgttca tggacggcat
      121 cttcgctcat ttgaacttcg cggtcttcca gaccatggtc gtttcgctgg agatgctgat
      181 cgggctggcc ctgttcggag ggcttttcac ctggtgggcg gcggtggcct cgatcgggat
      241 gtgcctgctc ttcacgttca ccggcatgtt tgcctggaac caggcctggt tcattttcgc
      301 cgggatcctg atgatgggcg gggccggcag ggcgttcggc ctggactcct gggtggtgcc
      361 cttcgtgaag cgctggtgga acggcacccg gctggcccgc aagacctatc tgtacgcgga
      421 cgagcctacg aagtagccgc atgggttcct tctgggacgg gtacccggag ctggagcggg
      481 agctggaagc ggtccggacc ctgatcctgg atcgggcgac gacggacgac cgggacgtgc
      541 gctcggcgat cgcgcacctc ctgagctcga gcggcaagat gctgcgtccg gcgtttgtgc
      601 tcctggcttc ccgcttcgga acgccggacc ccgggcgcac gcgacgggtg gccgccgctg
      661 tcgagatgct ccacatggcc acccttgtcc acgacgacat catcgacggc gccagcaggc
      721 gccgtggcgt ccccaccctc aacgccacag ccggagcgcg gacggcggtc ctggttggag
      781 actggcttct ggcatccagc gtctcgctga tcgtggacct gggagagacc caggacgcgc
      841 gggcgttggc ggccattacc gcgcggatct gcgcgggaga gatcagccag tcgaccgatc
      901 gaggagtggt ccacaccagc gtccgacggt accttcgccg gatcgccgcc aagacggcgg
      961 cactgttctc cctgtcgttc tacgtcggcg cgcgggaaag cggctgcggc gaaccggtct
     1021 ccagcgtgct gcggagactg gggtattgcc tcgggatggg gttccagatc atcgacgaca
     1081 tcctggattt ccatcgcgaa ggggcgcgca cgggcaagcc gacgggcaac gacctggcgc
     1141 aggggatcta cacgctgccg gtcatcctgg ctcttcggac tgcggatggc ccccgactcc
     1201 gggcggtcct gcggcggcct ccgcggtcct cccactcggc agcccgcgcc gcagggctgg
     1261 tccgagacgt cggcggcatc gacgctgccc ggcgcgtggc ggccgcctac acggagcgct
     1321 cccttcggga gatctcccgc ctgccggcca ctgacgcgcg tcggacgctg gaagtcgtaa
     1381 ccgctcgtct ccttcatcgc gaccactgag gatgcccgcc gacgtccgcc gggttggatc
     1441 tcggccttga ctttccggac caattctgcg agcgtcaaca aaacagttct cgaggaggaa
     1501 caacgatgcg gaagacagtg ctagctgcgt gtgttgcagc gctcttggtg ttcgggacgg
     1561 cgggagcatt tgccgcggga gaggtcaaga gcaccacgtt cccttggatg gcttcgtttg
     1621 accagccggg tcagctcaac ctcaacgtgt cggctgggtt ctatggcctt gggatagacg
     1681 tcaacgtcgg ccccgagttc atcatcggaa agttcgatat tgcgggcatt cccttgtcct
     1741 ggggagccac ggtgcgcgga ttggtcgggt tctcgagctt cttcggggta agctggatcg
     1801 actgggcggc ggcgcccatg gtcaccctgc actggggagt tgacttcgga gcgccctgga
     1861 agttcgactg gtacgtcgga gccggactca gcatctccgg aaccacgggc acctactact
     1921 cgaccctggg actcgcgcag ggagccgttg ggttcggatt cgcctcggcg gacggtgtgg
     1981 cctggcactt ctccaacaac atcgccctct tggcggagta cgcctacacg ccctacgtct
     2041 cgaccgcagg agtcggagtg aagttcgctc tctaggtccg ttctttcgcg gaagggcccc
     2101 ggttcctgcc ggggcccttt ccaggaccac aacgagaagt cagaatgaac ccaacgttgg
     2161 ccaagatcgt cattctggga atcccgctcc tgctttcgct gagctggttc gtctactggg
     2221 tcctgcgcat cacctttcat cagaagcgcc agaagctcct gggccgcaat cgggcctgac
     2281 ccggatcctg cctgcctccc gcctattcct cctgctcaag gcgccgctct ccggtcaacg
     2341 cctggagggg accgatatcc tgggtcacct cgagcgtccc caggaacttc cccgccgggt
     2401 ttcgcaccgc gaagtatcgg atatggaccc gcttgccgtg aaggtcgatc cagaagtcgg
     2461 cgtggtctcg cgcgcccgag cggaacgagg cgatgatccg gttgacccgc tccaggctct
     2521 tggggggatg gcaatcctga accgctctac ccaggacgga cgtggcgcgg acaaagatgc
     2581 ggtccttgga ctgggaaaag tagcggactc gatcttcagc atcgacaaag gagatgtcca
     2641 ccggtagcgt gtcgagcatc gccgtgagct gctccacggt gagggtcccg ctggggagcg
     2701 ccacggtgcc aggtgcggcg gtaggggcgg ccaccgatcc gccgccggat ccggccgccg
     2761 atccgctctc cagagcctct gcctcctcca gcctctgatg gagtcccgcg gtgcccgcgt
     2821 atggataacc gatctcctcc gctcctttga gcacctggac ccactccgag gtatcgatgc
     2881 gctccagagc ggcggggaag aggatcttct cctccttgaa gaccatgccg tccacctcgg
     2941 ccgtcagcgc cgaaagcgcg ccggggtccg gcatctcgtt cggggcgccc gcctccgctc
     3001 tcttcaggag cgcgcggatc tcgtcgtgct tgctccacat gactttggac ggtcccgtga
     3061 acccgtgccg ctccaggaac gggaagatcg cgttctcctt gatggcatag tggcggtcca
     3121 ctccgcgaag ctcggtgaga gccgaacgcg cgcccgccgc gtctcccgtg gacagggcct
     3181 tcttgagccg cgcgctgatc tcctcgatgt gacggttctc ccgggtcagg gcgttcaccg
     3241 ggtgtgctgg ctgatccggc gtggccacgg cttgttccag agcgtgctcg aagaggagcg
     3301 cgtggacgtt gcagaacctc ttgatctcct ccgcgggcag tccctcctcg atcaacgact
     3361 gttcgatgcc ggtgatctcc gcggatgaga gtctgccaac ctcttcgagg atccgatcct
     3421 ttgccttctc gacgggcagc ccttggtgaa gctgccggat gatatcgcca accagtttct
     3481 tctttctctc gtccaaagac cgcatctcgt tcctcctggc agaagcgtat ccgcctcctg
     3541 gccgctcgac cttgactcag gtcaagccgc ggcagctata gtaccgggaa aggtcctgac
     3601 ccgtacatgc atcctgatga gacgacccat ctccacctga gggagctgcg cttccgctac
     3661 ccggacgagg tcggcatgcc tggccgtcaa gtcctggatg ggctatccct gtctgtccgc
     3721 cgcggcgcgg tggcggtgct gctcggcgca gccgacgcgg gaaagaccac cgtgagccga
     3781 ctcatcgccg cccagattcc ccggttcacg ggaggagagg tttccggatc catcgagctc
     3841 cacggcagag acctgaccgg cgccaaaccg tacgagctcc tggagctcgt gggcgtagtc
     3901 tcccaggact ccaatgagca gatcatcacc gcgcgatgcg acaccgaggt ggcgtttgcc
     3961 ctggaatccc tgggtctccc tcgacgccag atcgtcgagc gtgtggacga ggctctggac
     4021 ctgctggggt tgagcgggtt tc
//
LOCUS       DASRZS010000605         6075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3039901, whole genome shotgun sequence.
ACCESSION   DASRZS010000605 DASRZS010000000
VERSION     DASRZS010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3039901"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..962)
                     /locus_tag="VFH83_14710"
     CDS             complement(168..962)
                     /locus_tag="VFH83_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487676.1"
                     /translation="MALKTVRIGSLDVTRLVIGGNPFSGFSHQGPARDKEMRRYYTVD
                     RIKRTLRKAEEAGINTFFGRADNHVIRMLEEYWAEGGRIQWFAQTASEIDDYVRNIST
                     AAANGAKAVYLHGGVADFYINKQQTEHFDKALCQARKLGLPGGLAGHKLETHCWAREH
                     LSPDFELVCYYDPIPREDDPHHKAGDYEVFDPAHRDAVVKLIPGMKWPVVHYKVLAGG
                     RTPAKEAFTYAAKHVRPQDIVLVGFHLGDDEDIIAKTVDLFNKTIG"
     gene            complement(1035..1823)
                     /locus_tag="VFH83_14715"
     CDS             complement(1035..1823)
                     /locus_tag="VFH83_14715"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6487677.1"
                     /translation="MKLSMLTYDLARPWTLTKLIEVARACGYAGIEFRAEEGHQHSVE
                     LDRTKAERRRIREQLEDAYLEAVGIGTGCRFDSTEPSERKANVEHARRYVELAADVGA
                     HRIRVFGDDLKAATNREDCIRYVGESLRAVAEFAQPLGVDVLLEMHGQFNYWGFTRAA
                     VEAAGHPRVALVYNCDVRDLVAGSVATTYARVRGLIRHVHMHTFYDGFPYPELLSLLA
                     ADGYTGYLSSEIEGIENPTREQYFAMYAALFRAWAGQPFWPGGV"
     gene            complement(1914..2186)
                     /locus_tag="VFH83_14720"
     CDS             complement(1914..2186)
                     /locus_tag="VFH83_14720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HET6487678.1"
                     /translation="MQQDQLRFSGLHELRAHLAEAVNRAAYGHQPTVVLRRRTKLAVV
                     VSIDDFLFLERMKQRRAEALSEEPPKDPARVGAYLARKLEAEMRYG"
     gene            2475..3257
                     /locus_tag="VFH83_14725"
     CDS             2475..3257
                     /locus_tag="VFH83_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6487679.1"
                     /translation="MAGITFSVFTKPWKDLGMSELGAFVERLGFEGIEFPLRPGYQVA
                     PENAEKGLPELARVLGDHGVKITSVAGPTDERTFAACAKAGVPVIRIMLRLEKENYLE
                     AEARFHRELQAMVPLCKRYGVTVGVQNHCGEFVNGGTGLRRMLEPYDPKQIAAVWDAA
                     HIGLGGEEVELSLDLVWSHLCMVNLKNAFWRRVNGPEATVAQWEWFWTSGRQGLASWP
                     RIARYLKEKSYRGVICLTAEYTAEDQVNRLIAEDIAFARSLL"
     gene            3290..4348
                     /locus_tag="VFH83_14730"
     CDS             3290..4348
                     /locus_tag="VFH83_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6487680.1"
                     /translation="MSSQERVRTAIDHKTPDRVPIMDGPWGATVRRWEGEGLPEGRSP
                     ADHFGYEIVAIGADTTPRFPTRVLEENDEYITETTSIGGIRRNHRDYSTTPEVIECPI
                     KKPEDWPAIRERLKPDLKRMDWASAWSTYQNARERGMYVVYSSACGYDLLQSYVRSEQ
                     LLNYMALEPEFVKEMVDVTSDLSLECARMMVKEGFAFDGSWYYDDMGYRNSSLFSPRM
                     YQEIIAPADRRRNDWFHENGMQTILHSCGCVKGLIPSLIDAGFDCLQPLEVKAGMDLR
                     ELKPKHGKDIAFFGGINTMLMEAAEPERIEEEIRVKFKAAMPGGGYLYHSDHSIPKDV
                     SFERYTFVMDCVRRYGAY"
     gene            4351..4635
                     /locus_tag="VFH83_14735"
     CDS             4351..4635
                     /locus_tag="VFH83_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019476724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HET6487681.1"
                     /translation="MGINLQILGSTFLMIFLAELGDKTQISTFAFASETRSPLSVFLG
                     AAGALVCTSFLGVVVGGVAGRFVPERIMKLAAALVFLGFGGWTLIQAIRG"
     gene            4639..5124
                     /locus_tag="VFH83_14740"
     CDS             4639..5124
                     /locus_tag="VFH83_14740"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET6487682.1"
                     /translation="MAGPIARWILRRVIKSAMAFETDSIELYRRLVGQMAGEAAPSKS
                     CREELHASLCHLMEEEQEHWRLLQDASTGRLSVEELERLLASHRYAQYESIEPLPPQE
                     LALWGTELARALAQEEKTLIFYGNLRRMSKIPVVKRAFEVLAAMEREHLEILRRLLGK
                     G"
     gene            5212..>6075
                     /locus_tag="VFH83_14745"
     CDS             5212..>6075
                     /locus_tag="VFH83_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET6487683.1"
                     /translation="MSTEVDLAQIKRAAHNAIPLSFKTYTLPHETEVFLDKILESFLA
                     ELGQAKLKEPLSYCLRELAVNAKKANTKRVYFQEKGLDLNNEKDYESGMKTFKQDTFD
                     NIQFYLQKQKELGLYIRVVFQTQGKSLKITVRNNVEITRKEQIRVFDRLARSRAFSSL
                     EEAFSTVLDSTEGAGLGIVILVLMMKKIGLSEDNFSIDAEQGETVAALTVPFSDVHLE
                     KLETLTQLLVKEVETLPQFPENILNLQKLVGNDAAEVNDIAREISVDPIMTADLLKLV
                     NSAQFMVPKRVD"
BASE COUNT         1107 a         1901 c         1977 g         1090 t
ORIGIN      
        1 ggccgcctcc gcggcccacg gggccggggc ctggatggcc gcgcaggtca ggatacccga
       61 ctcgaagcac ttcctcacgg ccacgttgca gccgtgggtc atgcccatgt cgtcggcgcg
      121 gacaatgagc cggatcagct ccggtgagtc tgcggacatc cgcgtgctta tccgatggtc
      181 ttgttgaaga ggtctaccgt cttggcgatg atgtcctcat cgtctccgag gtggaatccg
      241 acgaggacga tatcctgcgg ccggacgtgc ttcgcggcgt aggtgaacgc ttccttggcg
      301 ggggtgcggc cgccggccag caccttgtag tggaccacgg gccacttcat gccagggatc
      361 agcttcacca cggcgtcccg gtgggcggga tcgaatacct cgtagtctcc ggccttgtgg
      421 tgcgggtcgt cctcccgggg gatcgggtca tagtagcaga ccagctcgaa gtccgggctc
      481 aggtgttccc gggcccagca gtgcgtttcc agcttgtgtc ccgccagacc tccgggcaga
      541 cccagcttgc gggcctggca gagcgccttg tcgaagtgct cggtctgctg cttgttgatg
      601 tagaagtccg cgaccccgcc gtggagatag acggccttgg cgccgttggc cgctgccgtg
      661 gagatgttcc taacgtagtc gtcgatttcc gacgcggtct gggcgaacca ctggatgcgg
      721 ccgccttccg cccagtactc ctccagcatc cggatgacgt ggttgtccgc ccggccgaag
      781 aaggtgttga ttccggcctc ctccgccttg cgcagcgtgc gcttgatgcg gtccaccgtg
      841 tagtagcgcc gcatttcctt gtcccgcgcg ggaccttgat gggagaagcc gctgaagggg
      901 ttaccgccga tgacgagccg ggtaacgtcg agcgatccga tgcgtaccgt cttgagagcc
      961 acagccaacc tccgagcacc aggattctat tccacacgcg cgcgggggtc aagctacccg
     1021 cgatttcagg gagctcagac ccctcccggc cagaacggct ggcccgccca ggcgcggaag
     1081 agcgccgcgt acatggcgaa gtactgctct cgggtggggt tctcgatccc ctcgatttcc
     1141 gatgacagat accccgtata cccgtccgcc gccaggagag agaggagctc cggatacggg
     1201 aacccgtcgt agaacgtgtg catgtgaacg tgccggatca ggccgcggac ccgggcatac
     1261 gtcgtggcca ccgacccggc gaccaggtcc cgaacgtcgc agttgtacac cagcgccacc
     1321 cgaggatgcc ccgcggcttc cacggccgcg cgtgtgaacc cccagtagtt gaactggccg
     1381 tgcatctcca ggagcacgtc cacgccgaga ggctgagcga actccgctac cgctcgaagg
     1441 ctctccccca cgtaccggat gcagtcttcc cggttcgtgg cggctttcag gtcatcaccg
     1501 aacacccgga tccggtgggc gccgacatcc gcggccagct cgacgtagcg cctggcgtgc
     1561 tcgacgttgg ctttgcgctc ggacggctcg gtcgagtcga accggcaccc cgtcccaatg
     1621 cccaccgcct cgaggtatgc gtcctcgagc tgctcccgga tgcgccgccg ctccgctttg
     1681 gtccggtcga gctccaccga gtgctggtgg ccttcctccg cgcggaactc gatacccgcg
     1741 tacccgcacg ctcgcgcgac ctcgatcagc ttggtcaagg tccatggcct ggccaggtca
     1801 taggtgagca tgctcagctt catggtgtct cccgtctgcc gcggcggact ccggcggcgt
     1861 tcgcgggcgc atcctaccgg cgccgcaagc cccctgtcaa tcatccgaaa cgatcaccca
     1921 tagcgcattt ctgcctccag cttccgcgca agataggctc cgacccgtgc cgggtctttc
     1981 ggtggctcct cactcagcgc ctccgcccgc cgttgcttca tcctctcaag gaagaggaag
     2041 tcgtcaatgg aaaccaccac cgcaagcttg gtccttctcc gcaacaccac ggtgggttgg
     2101 tgcccatagg ctgcgcgatt gactgcctcc gccaggtgcg cccgcagctc atgcaggccg
     2161 gagaaccgaa gctgatcctg ctgcatcgca ccggatatca cgggtcaccg cgtcgttctg
     2221 attgagcgct gtcgtggact tccggccgtc gaacggtacc tttgcgcatg ttcacgcata
     2281 ttggttccgg gcgaacgcat cgccggaaac aacgccgggg cgtgagacac atcgagtgaa
     2341 cgtgcgcaaa ggtaccactg tggactgccg gccccgaggt gttgcggcct tgtgggttgc
     2401 cggcgcctgg tcctcgctct ggacatcgcc ctggatacct gcgaacctgg gctcaatacc
     2461 gcgaggagga agggatggct ggcatcacgt tctcggtgtt caccaaaccg tggaaggatc
     2521 tggggatgag cgagcttggg gcgttcgtag agcgcctcgg gttcgaagga atagagtttc
     2581 cccttcggcc aggatatcag gtggccccgg agaacgcgga gaagggactc cccgagctgg
     2641 cgcgcgtcct cggagaccac ggggtgaaga tcacgagcgt agcgggccct acggacgaga
     2701 gaaccttcgc cgcctgtgcg aaggctggcg tgccggtcat tcggatcatg cttcgcctgg
     2761 agaaggagaa ctacctggag gcggaggcgc gtttccatcg ggagctccag gcgatggtgc
     2821 ccctctgcaa gcggtatggc gtcacggtgg gcgttcagaa tcactgcggc gagttcgtca
     2881 acggcgggac gggactgcgt cggatgctgg agccgtacga cccgaagcag atcgcggcgg
     2941 tctgggacgc cgcccacatc gggcttggcg gtgaggaggt cgagctgtcg ctggacctgg
     3001 tctggtccca cctgtgcatg gtgaacctca agaacgcgtt ctggcgacgg gtgaacggtc
     3061 ccgaggccac ggttgcgcag tgggagtggt tctggaccag cggacgccag ggactggcct
     3121 cctggccgcg gatcgcgagg tatctcaagg agaagtctta tcgcggcgtg atctgcctga
     3181 ccgcggagta caccgccgag gaccaggtga accggctgat cgccgaggac atcgcgttcg
     3241 cgcgatcgct cctctgatcg ttcttcgaga gaaaggaaac gccatggcaa tgagctcaca
     3301 ggaacgggtt cgcaccgcga tcgaccacaa gactcccgac cgcgtgccca tcatggacgg
     3361 cccgtgggga gctacggtcc gacggtggga gggagaggga ctaccggagg gcaggagccc
     3421 cgccgaccac ttcggctacg agatcgtggc gatcggcgcc gacaccaccc cgaggttccc
     3481 gacccgcgtg ctcgaggaga acgacgagta catcacggaa acgacctcca tcggcgggat
     3541 ccgcaggaac catcgggact actcgaccac gccggaggtc atcgagtgtc cgatcaagaa
     3601 gccggaggac tggccggcca ttcgggaacg gctcaagccg gacctcaagc gcatggattg
     3661 ggcgagcgcg tggagcacgt accagaacgc ccgggagcgc gggatgtatg tcgtctactc
     3721 cagcgcgtgc ggctacgacc tgctgcagtc ctacgtgcgc agcgagcagc tcctgaacta
     3781 catggccctg gagccggagt tcgtcaagga gatggtggac gtgacctccg acctgagcct
     3841 ggagtgcgcc cggatgatgg tgaaggaagg attcgcgttc gacggatcct ggtattacga
     3901 cgacatgggc tatcgcaact cctccctgtt ctctccccgg atgtaccagg agatcatcgc
     3961 cccggcggac cgacgccgga acgactggtt tcacgagaac ggaatgcaga ccatcctgca
     4021 ctcgtgcggc tgtgtgaagg gtctcatccc gtccctcatc gatgcgggat ttgactgcct
     4081 gcagcctctg gaggtgaagg cggggatgga cttgagggag ctcaagccga aacacggcaa
     4141 ggacatcgcg tttttcggcg ggatcaacac gatgctcatg gaggcggcgg agccggagag
     4201 gatcgaggag gagatccggg tgaagttcaa ggcggccatg ccgggcggcg ggtacctgta
     4261 ccattcggat cactccatcc caaaggacgt gagcttcgaa cggtacacct tcgtcatgga
     4321 ctgcgtccgc agatacgggg cctactgagc atgggaatca atctccagat cctgggttcc
     4381 acgttcctca tgattttcct ggccgagctc ggtgacaaga cgcagatctc gactttcgcg
     4441 ttcgcttccg agacacggtc tcccctgtcg gtgttcctgg gggcggccgg cgccctggtc
     4501 tgcacctcgt tcctcggcgt agtggtaggc ggagtggcgg ggaggttcgt gccggagcgc
     4561 atcatgaagc tcgccgccgc gctggtgttc ctggggttcg gcggctggac cctgatccag
     4621 gctatccggg ggtaggggat ggccgggccg atcgcgcgct ggatcctccg tcgggtgatc
     4681 aagagcgcca tggcgttcga gacggattcc atcgagctgt accgccgtct ggtgggtcag
     4741 atggcgggag aggctgcgcc atccaagtcg tgtcgggagg agctccatgc gagcctgtgc
     4801 catctcatgg aggaggagca agagcactgg cgtcttctgc aagatgcttc caccggccgc
     4861 ttgtccgtgg aggagttgga gcggcttctg gcgagtcatc gctacgccca atacgaatcc
     4921 atcgagcctc tgccgccgca ggagctcgcg ctctggggga ccgagttggc ccgggcgctg
     4981 gcgcaggaag agaagacgtt gattttctac gggaaccttc gtcgcatgag caagatcccg
     5041 gtggtgaagc gggccttcga ggtgctcgcc gccatggaga gggagcacct ggagatcctc
     5101 cgcaggctcc tggggaaggg ctgaccgccc ggtttggcta tatcaccttg acagcgcttg
     5161 gcggttccac tacgctagaa atcgagtaag cgccagcaaa aaaggagccg catgtccacg
     5221 gaagtcgacc tcgctcagat caagcgggcc gctcacaacg ccattcctct cagtttcaaa
     5281 acctacacgt tgccgcacga gaccgaggtc ttcctggaca agatcctcga gagcttcctt
     5341 gccgagctgg gccaggccaa gctcaaggag ccgctctcct attgcctgcg agagctcgcc
     5401 gtcaacgcca agaaggcgaa caccaagcgc gtgtacttcc aggagaaggg cctggacctg
     5461 aacaacgaga aggactacga gtccgggatg aagacgttca agcaggacac cttcgacaac
     5521 atccagttct atcttcagaa gcagaaggag ctggggcttt acatccgcgt cgtcttccag
     5581 acccagggca agagcctcaa gatcaccgtc cgcaacaacg tggagatcac gcgcaaggaa
     5641 cagatccgcg tcttcgaccg cctggcccgt tcccgagcgt tcagctccct ggaggaagcc
     5701 ttctccaccg tgctggacag cacggaaggc gcggggctgg gcatcgtgat cctcgtcctg
     5761 atgatgaaga agatcgggct ttcggaagac aacttctcca tcgacgctga gcagggggag
     5821 acggtggccg ccctcaccgt cccgttctcc gacgtgcacc tggagaagct ggagaccctg
     5881 acgcagctcc tggtaaagga agtggagacc ctcccgcagt tccccgagaa catcctcaac
     5941 ctccagaagc tcgtcgggaa cgacgcggcg gaggtgaacg acatcgcccg agagatcagc
     6001 gtggacccca tcatgaccgc ggacctcctg aagctcgtca actcggcgca gttcatggtc
     6061 cccaagcgcg tggac
//
LOCUS       DASRZS010000606         3877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1506037, whole genome shotgun sequence.
ACCESSION   DASRZS010000606 DASRZS010000000
VERSION     DASRZS010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506037"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VFH83_14750"
     CDS             complement(<1..792)
                     /locus_tag="VFH83_14750"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET6487684.1"
                     /translation="MYAVALIAISAAPALAFLLLILRMDRQEPEPLGFVLRILALGAA
                     SCMVAAVVERALDLFPLFHAPGFAGHAAVSFVEVAPIEELCKLLVVLLFVWGNPNFNE
                     ENDGVVYVGASAIGFALLENVLAVAQHGLGTGLLRAFTSIPLHVFVGVVLGLNVGLAR
                     FARTPQRRDLRILWGFLLAWFFHGAYDTFALSGTELVILLLPLLAGLAAIGVAALRRG
                     RLLSLLRWRGVVRAPAPPHQRRSHRWMPIVSRTLLGGCLFFWIVLA"
     gene            complement(823..2262)
                     /locus_tag="VFH83_14755"
     CDS             complement(823..2262)
                     /locus_tag="VFH83_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HET6487685.1"
                     /translation="MAKITLGLIVGNRDVFPAELARSGRDEMIPLLQGQDIDIVAVTP
                     QDTTDGVVLTWKDAEKCAELFRQNASKIDGILATLPNFGDERAVADSIKRSGLTVPVF
                     IHAYPDTVDGMSIKNRRDAFCGKLSVCNNLKQYGIPYSIGPEHVLSPQSPRFKEELSW
                     FVDVCRVVKGLRGARIGSIGERTTPFKTVRYSEKLLEQSGISVESKSLVETVSEIEKL
                     KDTDQPVVAKLERLRGYLPGSREIPPRPLLTTAKLGVVLERWVREYRINSYAIQCWSA
                     MQDALKIFPCSIMSMMSNELLPSACEVDVMGAVAMYALQLAGGGASALFDWNNNYGED
                     PDKLVLFHCSNTAISFMKDVKPGFNAMAVKGNPLDSCFCTLHGSLKPGKVGWARFSTD
                     DTKGRIVGAIGDGDVTEETLDSFGTTGVMRTPNLQKLLYFLAVNGFEHHVALNYSPRT
                     SALYEALTRYMGWKIYHHNGEDRTLFESW"
     gene            complement(2362..3132)
                     /locus_tag="VFH83_14760"
     CDS             complement(2362..3132)
                     /locus_tag="VFH83_14760"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET6487686.1"
                     /translation="MILERFSLKGRTAVVTGAGQGLGRVFALSLADAGADIVVAELSA
                     QTGQRVAEEIRALGRRALFHPTDVREPASIEAMVEASIKELGSIDVLVNNAGIANWCA
                     AEQVPEKDWRAVMDVNLNGVFFCSQQVARRMIERRTGCIINIASMSGLIVNRPQPQSA
                     YNASKAAVIHLTRSLAAEWAPYGIRVNAIAPGYMATPMAEPYFKDQRYGGVWMDMTPL
                     GRPGQPEELGPLAVFLASEASSFVTGSVMVADGGYTAW"
     gene            complement(3129..>3877)
                     /locus_tag="VFH83_14765"
     CDS             complement(3129..>3877)
                     /locus_tag="VFH83_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET6487687.1"
                     /translation="GCWQVFTDEITRKVLDRGYILAHFNRTELATDVRSKARDGGLYP
                     FFPKRDFGALAAWAWGFHRCVDALIAMPEVDARRVIATGHSRGGKTALLAGATDERIA
                     LTAPNDSGCGGAGSFLCQGPESETLADILRVFPYWFSSKLPAYLRKGRTLPFDQHLVK
                     ALVAPRALLATEALGDLWANPTGTHHTHEQAREVYRLLGAEDQIGLCYRPGGHEHALE
                     DWNALLDFADWRLGGSERRRDFWQSPGGTP"
BASE COUNT          655 a         1315 c         1279 g          628 t
ORIGIN      
        1 cgccagcacg atccagaaga agaggcagcc gcccaggagg gtccgcgaga cgataggcat
       61 ccagcggtgg gaccgcctct ggtgcggggg cgccggggcg cggacaaccc cccgccagcg
      121 caggagcgag agcaggcgac cccgccgcaa cgccgccacc ccgattgccg caaggcctgc
      181 gaggagcgga aggaggagga tgaccagctc cgtgccggac agcgcaaagg tgtcgtaggc
      241 gccgtggaag aaccacgcca gcaggaaccc ccaaaggatc cgcaggtctc gccgctgcgg
      301 cgttctggcg aaccgggcca ggccgacgtt cagacccagg accacgccga caaagacgtg
      361 cagcggaatg gaggtgaatg cgcggagcaa gcccgtaccc agcccgtgtt gggccaccgc
      421 caggacgttc tccaagagcg caaaaccgat ggcgctcgcg cccacgtaga ccacgccatc
      481 gttctcctcg ttgaagttcg ggttccccca tacaaagagc agcaccacca ggagcttgca
      541 cagctcctcg atgggcgcaa cctctacgaa cgagaccgcg gcatggcccg cgaaaccggg
      601 cgcgtggaag agcgggaaga ggtccagcgc gcgctcgacg acggccgcca ccatgcagct
      661 tgccgcgccg agcgccagga tgcggagcac gaaccccagg ggctcgggct cctggcggtc
      721 catccgaagg atgaggagca ggaaggcgag ggcgggggcc gcggagatcg cgatgagcgc
      781 aacggcgtac attccgctcc ctcgccgtct ctgaggattc cgctaccagc tctcgaagag
      841 ggtccgatcc tcgccgttgt ggtggtagat cttccagccc atgtagcgcg tcagcgcctc
      901 gtacaaggcc gaggtccgcg gtgagtagtt gagcgcgacg tggtgctcga aaccgttgac
      961 ggcaaggaag tacaggagct tctggaggtt gggggttctc ataacgccgg tggtgccgaa
     1021 gctgtccagc gtctcctccg ttacgtcacc atccccgatg gcgccgacga tccgcccctt
     1081 ggtgtcatcc gtggagaacc gcgcccagcc gaccttgccg ggtttcaggc tgccgtggag
     1141 cgtgcaaaag cacgagtcca aggggttccc cttgacggcc atggcgttga acccgggctt
     1201 cacgtccttc atgaaggaga tggccgtgtt gctgcagtgg aagagcacca gcttgtccgg
     1261 gtcctcgccg tagttgttgt tccagtcgaa gagcgcggaa gcgccccctc cggcgagctg
     1321 cagcgcgtac atcgccacgg cccccatgac gtccacctcg caggcggacg gcaggagctc
     1381 gttgctcatc atgctcatga tggagcaggg gaagatcttg agcgcgtcct gcatggcgga
     1441 ccagcactgg atggcgtagg agttgatccg gtactcccgg acccagcgct ccagcaccac
     1501 ccccagcttg gcggtggtca ggaggggccg tggcggtatc tcccggctgc cgggcaggta
     1561 gccgcggagc ctctccagct tggcaacgac cggctgatcc gtgtccttga gcttttcgat
     1621 ctcggacacc gtctccacca ggctcttgga ctccacgctg atgccgctct gctccaggag
     1681 cttttcgctg tagcggaccg tcttgaaggg tgtggtccgc tcgccgatgg acccgatccg
     1741 cgccccgcgc aggcctttca ccacgcggca tacgtccacg aaccaggaga gctcttcctt
     1801 gaaccggggg ctctgcgggg agagcacgtg ctccggaccg atggagtacg ggatcccata
     1861 ctgcttcagg ttgttgcaga cggagagctt gccgcagaac gcgtcccgcc ggttcttgat
     1921 cgacatcccg tccaccgtgt cggggtatgc gtggatgaac acgggcaccg tcagtccgga
     1981 gcgcttgatg gagtccgcaa cggcccgctc gtctccgaag ttcggaagcg tggccaggat
     2041 gccatcgatc ttcgacgcgt tctggcggaa gagctccgcg cacttctccg cgtccttcca
     2101 ggtcaggacc accccgtccg ttgtgtcctg cggggtcacc gccacgatat cgatatcctg
     2161 cccttggagc agggggatca tctcatcccg gccgctcctc gccagctccg ccgggaacac
     2221 gtcccggttt ccgacgatga ggcccagtgt gatctttgcc atgtgccctc ctgaggtcga
     2281 ctgcgagctg cgccatggtg tcacaggacc cggcgcggta tcaagatacg gcggcccggc
     2341 cgcgggcgcg caggcgatac cctaccaggc cgtgtagccg ccgtccgcca ccatgaccga
     2401 gccggtcacg aagctggatg cctccgaggc aaggaagacg gcgagcggcc ccagctcttc
     2461 cggctgccct ggccggccca gcggcgtcat gtccatccag accccgccgt agcgctggtc
     2521 cttgaagtag ggctccgcca tcggagtggc catgtagccg ggcgcgatgg cgttgacccg
     2581 gatgccgtac ggggcccatt ccgccgccag ggaccgggtc aggtggatca ccgccgcctt
     2641 ggacgcgttg taagccgact gcggttgggg ccgattcacg ataagcccgg acatcgaggc
     2701 gatgttgatg atgcagcccg ttcgccgctc gatcatgcgg cgcgccacct gctggctgca
     2761 gaagaacact ccgttgaggt tcacgtccat gaccgcgcgc cagtccttct cggggacctg
     2821 ctcggcggcg caccagttcg cgatgccggc gttgttcacc agcacgtcga tggacccgag
     2881 ctccttgatc gaagcctcga ccatggcctc gatgctggcg ggctctcgaa cgtcggtggg
     2941 gtggaacagg gcccggcgcc ccagcgcgcg gatctcttcc gccacccgct ggcccgtctg
     3001 ggccgacagc tccgccacca cgatgtccgc gccggcgtcc gccagcgaga gcgcgaacac
     3061 gcgccccagt ccttgtcccg cgcccgtgac cacggccgtc cggcccttga gggagaatcg
     3121 ttcgaggatc atggggtgcc tcccgggctc tgccagaagt cgcgccggcg ctcggatcct
     3181 cccaggcgcc agtcggcgaa gtccagcagc gcgttccagt cttccagcgc gtgctcgtga
     3241 ccacccggcc ggtagcagag gccgatctgg tcttcggcgc cgaggaggcg atatacctcc
     3301 cgcgcctgct cgtgggtatg gtgggtcccg gtcgggtttg cccagagatc tcccagggcc
     3361 tcggtagcca gcagcgcccg aggcgcgacc agtgctttca ccaggtgctg atcgaacggg
     3421 agcgtcctgc ccttgcgcag gtatgcgggc agcttcgacg agaaccagta cgggaacacc
     3481 ctcaggatgt ccgcaagggt ctcgctctcc ggtccctggc agagaaagga cccggcgcct
     3541 ccgcatcccg agtcgttggg cgccgtcagg gcgatccgct catccgtcgc tccggccagc
     3601 agcgccgtct tgcccccgcg ggaatggccg gtggcgatga cccggcgagc gtccacctcg
     3661 ggcatcgcga tcagcgcgtc cacgcaccga tggaatcccc acgcccatgc cgccagcgcg
     3721 ccgaagtccc gcttggggaa gaacgggtac agcccgccgt cccgggcttt ggatcgaacg
     3781 tccgttgcca gctccgtccg attgaaatga gcaaggatgt aaccgcggtc gagcaccttg
     3841 cgagtgatct cgtcggtgaa gacctgccag cagccgt
//
LOCUS       DASRZS010000607         8089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_718633, whole genome shotgun sequence.
ACCESSION   DASRZS010000607 DASRZS010000000
VERSION     DASRZS010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8089
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718633"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFH83_14770"
     CDS             <1..358
                     /locus_tag="VFH83_14770"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6487688.1"
                     /translation="VAARGGLLAEGPRMHYSERITLGRSSMEWRASHILVKDKQLAND
                     LLKKAKLGASFEALAREHSTCPSKSRGGDLGWFGPGKMVPAFENACKKLSSGSLSDVV
                     STQFGFHIIKLTGRKD"
     gene            419..1099
                     /locus_tag="VFH83_14775"
     CDS             419..1099
                     /locus_tag="VFH83_14775"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6487689.1"
                     /translation="MESLQCCGIEQVFDDNLAATEVKRHRMRGPRKTTQVLIDYLAAQ
                     GLAGATVLDVGGGVGALHQALLAAGAGRATDVDASAAYLRAAKSEALRKGVLERISFL
                     HGDFVALAPTIDAADIVTLDRVICCYDDAESLVALSAARARRYYALVYPWDSPLARAF
                     SVLLNGMRRLQRTFYRSYIHPRARVEDILRANGLERQFLRRAGLWQVAVYARVRPPGS
                     SSPLSSPA"
     gene            complement(1083..2444)
                     /locus_tag="VFH83_14780"
     CDS             complement(1083..2444)
                     /locus_tag="VFH83_14780"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HET6487690.1"
                     /translation="MSGLLLKDCFCIQPSADEPWTQGNDILIQGNRIERVAPNIPTPP
                     DTRAIDCSGCVVVPGLVNTHHHFFQTLTRNLPAVQNAKLFDWLVHLYEIWRRIDGEAV
                     YFSSLLAMGELLKTGCTCTTDHLYLYPRGAGDDFMELQFQAAEELGMRFSPTRGSMSR
                     GKKDGGLPPDSVVQEEDVILADSARAIDRFHDPAPLSMRKVGLAPCSPFSVSEDLMRE
                     SARLARRHGVRLHTHLAETDDEDAYCMRAYGVRPVQLMERCEFIGPDVFYAHGIFFQD
                     HELAVLRDRRAQVAHCPSSNMRLGSGICRVRDMLDLGVPVSLAVDGSASNDSSDMLGE
                     MRHALLLQRVARGSGALTAREVLRMATETGAAVLGFGTLGRIQPGWGADLAVFDVNRL
                     EYCGSLSDPFAALLFAGYDHGARHTICNGRLVVENGRLTGFDEERLIREANRIAGKLT
                     RAS"
     gene            complement(2441..3235)
                     /locus_tag="VFH83_14785"
     CDS             complement(2441..3235)
                     /locus_tag="VFH83_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6487691.1"
                     /translation="MVFADRYGPWAVVAGGSEGLGAAIAVELARKGLHLFLIARRPAP
                     LAETSARIRADHGVQVKTLAHDLADPAFPGVLRREIADLDVGLLVCNAAVAFTGPFLK
                     PEASLYDRIVDVNCKTSLALTRMAAELLTKRGRGGILLMSSMAGFQGSPYVAVYGATK
                     SFLLSLAEALGEELGPRGVDVMACCPAAVLTPNYASAEGAGGAKVPFSLKPEQVAREA
                     VAGLGRRRVVIPGAGPRLAHLLMSRLMPRGAAVSMMGRSTKGLMAP"
     gene            3376..4245
                     /locus_tag="VFH83_14790"
     CDS             3376..4245
                     /locus_tag="VFH83_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET6487692.1"
                     /translation="MIKETRRDASAGVGILILFLAIEVALVVVFVWSAPRGLPWVTGA
                     AALAFGVVGFLLSGLFTVSPNQGRVLQLFGAYRGTAREPGLRWANPFYTKRAISLRVR
                     NFETGKLKVNDKRGNPVEIAAIVVWNVVDTAEATFQVDNYENYVQVQSESALRNLATG
                     YPYDAHKPDEVALSSHTAEVSAKLKVEIEERLQKAGVEVIETRVSHLAYSPEIAAAML
                     RRQQAAAVVAARQTIVDGAVGMVENALEMLAAKKVIELDEERKATMVSNLLVVLCSEQ
                     NTQPVINTGSLYR"
     gene            4277..4462
                     /locus_tag="VFH83_14795"
     CDS             4277..4462
                     /locus_tag="VFH83_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014048084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487693.1"
                     /translation="MTDKRSFLLRISPDLYEAVAAWAEEELRSMNGQIEFLLRRVLLE
                     AGRLPRRPPPSDGAAGA"
     gene            4494..4724
                     /locus_tag="VFH83_14800"
     CDS             4494..4724
                     /locus_tag="VFH83_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487694.1"
                     /translation="MTTTTPECPVCRVRMEEGHVLDAGNSNHLLRVSWTEGQPEKGLM
                     GYRVKGRRRLPTITWRCPRCGWLLWFAREEQT"
     gene            complement(4746..5861)
                     /locus_tag="VFH83_14805"
     CDS             complement(4746..5861)
                     /locus_tag="VFH83_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET6487695.1"
                     /translation="MGASVTSLDQVRRLAVTRTLFAPTTLRRALHRLGFVQADPIRAP
                     ARAQDLILFHRVKGYRAGDLERRYEELGVNEERFVNYGFVTSELQALMHPRPQVKVPA
                     EGGGSWYASQQRLARRVLAFVRERGEVHPQEVEERFAIGTIRNWFGGASNATTHLLEA
                     MLYHGVVRVVRRESGIRIYAEQRHGPGPRTEAERRERLDTLADAAIGLYAPLSFSRLR
                     FLIGHLRHAVPQWKADLPGVIQRAVSRLRHACIDGVDWYWPEGGAAAGAPEDRVRLLS
                     PFDPVVWDRSRFEILWGWTYRFEAYTPAAKRQRGYYALPLLWRDRVIGWGNVSVKGAE
                     LTWDVGFVGAKPRDRLFPLALEEELERLRSFLRLPAP"
     gene            complement(5926..7389)
                     /locus_tag="VFH83_14810"
     CDS             complement(5926..7389)
                     /locus_tag="VFH83_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487696.1"
                     /translation="MTTRGPLETTSPLGLNVPADCRGEVVQRHSNEHLSDRMNTFLLG
                     RSLTHLSLELNRSRQATLLRQERRFRSLLRHAYLHSEFYRRHYHSAGIRGGDLDAIPL
                     RDLPTITKSQVMESFDSLSCDPALRREGVERFVADPTTRGRRYLNRFAVVHSSGSSGT
                     VGLFVYGPEEWSILGALTISRVGRSSVRPLHRTRLAFVGATDGHYAGITLSREAPRFL
                     YEFRPLSIQAPIEQIVAELNAFQPDMINGYASGAHLLAIQQLHGRLRISPRAIVCSGD
                     PLTDLVRSHVQEAFGIEPINLYASSESICMGVQRNGGEDMSLFDDWHVFEIVDERLKE
                     VGPGETGRLVLTNLYNRTQPLIRYEMSDLLTRGDDSRADGHPFQRIRALSGRSEESLS
                     FVVNGQERKLSPHVLGEFFVPGLEKFQYEHHPPDLLLMRAKVRHDGAPVRDRIHEKMH
                     DILVGSGLHETLRFRLEIVDRIDNDARTGKFRLIERR"
     gene            7420..>8089
                     /locus_tag="VFH83_14815"
     CDS             7420..>8089
                     /locus_tag="VFH83_14815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6487697.1"
                     /translation="MNILSLDGVSKTLVDTPLFEQVSLGIDAGDKIGFIGRNGAGKST
                     FLKILTGELAADTGSVARNRTLTLSVLPQRPLFLPGTSLEEYCFQENADQAAPGSEEH
                     AAVADAYRSLCRELGLADPAARMDALSGGMIRKASIARCLARGANFLLLDEPTNHLDL
                     DTIEWLEDRLRGVSFGFILVTHDRYFLDGVCTAIMEIDDRAVRKYAGNYSVYLERRAE
                     REEVR"
BASE COUNT         1417 a         2663 c         2729 g         1280 t
ORIGIN      
        1 cgtggccgca aggggcggcc tgttggccga aggcccccgg atgcactata gtgaacgcat
       61 cacccttggg aggtcctcta tggaatggcg ggccagccac atcctggtca aagacaagca
      121 actggcgaac gatcttctca aaaaggccaa gctgggagcc agcttcgagg ccctggctcg
      181 agagcactcc acctgtccgt cgaagtcccg cggcggggac ctgggctggt tcgggcccgg
      241 caaaatggtg cccgctttcg agaatgcctg caagaagctg tcctccggat ccctgagcga
      301 cgtggtctcg acccagttcg gcttccacat catcaagctt accggccgca aggactgacg
      361 catcccgctt tgacgaccga gccctcccct gcctatcttc cggggagagg tagaatcggt
      421 ggagtccctt cagtgctgtg gtatcgaaca ggtcttcgac gacaacctgg ccgccacgga
      481 ggtgaagcgc caccggatgc gcggcccccg aaaaaccacc caggtcctca tagactacct
      541 agccgcgcag ggcctggcgg gagccacggt gctggacgtg ggcggcggcg tgggagcgct
      601 ccaccaggcg cttctggctg ccggagccgg gagggccacg gacgtggatg cctccgccgc
      661 ctacctgcgc gccgccaagt ctgaagcctt gcgcaagggc gtgctcgagc ggatcagctt
      721 cctgcacggc gatttcgtgg ccctggcgcc aaccatcgat gcggcggaca tcgtcaccct
      781 ggaccgggtg atctgctgct acgacgacgc ggagtccctg gttgccctct ccgcggcgcg
      841 agcccggcgg tactacgccc tggtctatcc gtgggactcc ccgctggcca gggcgttcag
      901 cgtcctgctc aacgggatgc gtcggctcca gcggaccttc taccgcagct acatccatcc
      961 ccgggcgcgc gtggaggata tcctccgcgc caacggtctg gagcggcagt ttctccgcag
     1021 agccgggctg tggcaggttg ccgtgtacgc gcgggtgcgc ccgccgggat cctcctcccc
     1081 gctcagctcg cccgcgtaag cttccccgcg atccggttcg cctctcggat cagcctctcc
     1141 tcatcgaagc cggtgagccg tccgttctcc accaccagcc ggccgttgca gatcgtgtgc
     1201 ctggccccgt ggtcgtagcc ggcgaacagc agcgcggcaa acggatcgga gaggctgccg
     1261 cagtattcga ggcggttcac gtcgaaaacg gcaaggtccg ctccccatcc gggctggatc
     1321 cttcccagcg ttccgaaccc cagcacggcc gcgccggtct ccgtggccat cctcagcact
     1381 tcccgagccg tgagcgcccc gctcccccgg gccacgcgct gcaagaggag ggcgtgacgc
     1441 atctccccga gcatgtccga gctgtcgttg gaggccgagc cgtccaccgc cagggaaacc
     1501 gggaccccga ggtccagcat gtctcggacc cggcagatgc cggatcccag gcgcatgttg
     1561 gaggacgggc agtgcgcaac ctgggctcga cgatcccgaa gcacggcgag ctcgtggtcc
     1621 tggaagaaga tcccgtgagc gtagaagacg tcggggccga tgaactcgca gcgctccatg
     1681 agctggaccg ggcgcacgcc ataggcccgc atgcaatagg cgtcctcgtc atcggtttcc
     1741 gccaggtgcg tgtggagacg caccccgtgt cggcgcgcca gacgcgcgga ctcccgcatg
     1801 agatcctcgc tgacggagaa cgggctgcaa ggcgcaagcc caacctttcg catcgacagg
     1861 ggggcgggat cgtggaagcg gtcgatggcg cgcgccgagt ccgccaggat cacgtcctct
     1921 tcctgcacca cggagtccgg cggaagcccc ccgtccttct tgccgcggga catggagcca
     1981 cgggtggggg agaatcgcat gcccagctct tccgccgcct ggaactggag ctccatgaaa
     2041 tcgtcaccgg cgccgcgggg atagaggtac aggtggtccg tcgtgcacgt gcagccggtc
     2101 ttcaggagct cacccatggc gaggagggac gagaagtaca cggcctcccc gtcgatcctc
     2161 ctccagatct cgtagaggtg caccagccaa tcgaagagct tggcgttctg caccgcgggg
     2221 aggttccgcg tcagggtctg gaagaagtga tggtgcgtgt tcacgaggcc gggcaccacg
     2281 acgcaccccg agcaatcgat ggcgcgggta tccggcgggg tcgggatgtt gggcgctacg
     2341 cgctcgatcc ggttcccctg gatcaggatg tcgtttccct gggtccacgg ctcgtccgcg
     2401 gacggctgga tgcagaagca gtctttcagg aggaggccgc tcatggagcc atcagcccct
     2461 tggtggatcg ccccatcatg gacaccgccg ctccccgcgg catgaggcga gacatgagga
     2521 gatgcgccag gcgaggtcca gcgcccggaa tcacgacgcg acgccgaccc aggccggcca
     2581 cggcctcgcg tgccacctgc tcgggcttca gggagaacgg caccttggcg ccgccggccc
     2641 cctccgcgga cgcatagttc ggggtgagaa cggccgcggg gcaacacgcc atgacgtcca
     2701 cgccccgggg tcccagctct tctcccaacg cctcggccag ggagaggagg aatgacttgg
     2761 tggcgccgta gacggccacg tacggggaac cctggaagcc agccatgctg gacatgagca
     2821 gaatgccgcc cctgcccctt ttggtcagga gctccgccgc catgcgggtc agcgcaaggc
     2881 tcgtcttgca gttgacgtcc acgatgcgat cgtacagcga tgcctcgggc ttgaggaacg
     2941 ggcccgtgaa agccaccgcc gcgttgcaca ccagaagccc cacgtcgagg tcagcgatct
     3001 cacgccgcag gaccccggga aaggcggggt ccgccaggtc gtgcgccagc gtcttcacct
     3061 gcacgccgtg gtccgcacgg atacgagctg aagtctcggc cagaggcgcc ggacgtcggg
     3121 cgatcaggaa gaggtggagc cccttacggg ccagctccac ggcgattgcc gccccgagtc
     3181 cttcggagcc tcccgccact actgcccacg gtccataccg atccgcgaat accatccctg
     3241 gcctcccggg agcatcatat cagatctgac acgttggcgg cacctccgta ggaatccaga
     3301 gggcgggcga ttacccagtc ttgacaacgt gaaatcaaag tgatatcatt acaatatcga
     3361 gaggagagcg gtaccatgat caaagagacg agacgtgatg cgagcgcggg cgtggggatc
     3421 ctgatcctgt tcctggcgat cgaggtagcc ctcgtggttg tcttcgtgtg gtcggctcca
     3481 cgggggttgc cctgggtgac gggggcggcg gccctggcct tcggcgtggt ggggttcctg
     3541 ctcagtgggc tgttcacggt cagcccgaac caagggcggg tccttcagct cttcggcgcg
     3601 taccggggta ccgcgcggga gccgggcttg aggtgggcca acccgttcta cacgaagcgg
     3661 gccatcagcc ttcgcgtgcg gaacttcgag accggcaagc tcaaggtcaa cgacaaacgc
     3721 ggcaaccccg tggagatcgc ggcaatcgtg gtctggaacg tggtggacac ggcggaggct
     3781 acgttccagg tggacaacta cgagaactac gtccaggtgc agagcgagtc cgcgctgcgc
     3841 aacctggcca ccggttaccc gtacgatgcc cacaagccgg acgaggtcgc gctctccagc
     3901 cacaccgcgg aagtctccgc gaagctcaag gtggagatcg aggaacggtt gcagaaagcg
     3961 ggcgtggagg tgatcgagac ccgggtgagc cacctggcgt actccccgga gatcgcggcc
     4021 gccatgctgc gccggcagca ggccgcggcg gtggtggcgg cgcgacagac catcgtggac
     4081 ggggcggtgg gcatggtgga gaacgcgctg gagatgctgg ccgcgaagaa ggtcatcgag
     4141 ctggacgagg agcgcaaggc cacgatggtg agcaacctcc tggtggtgct ctgcagcgag
     4201 cagaacaccc agccggtgat caacacgggt tccctctacc ggtagccgga gaccgaccgc
     4261 ggggcggagg taagccgtga ccgacaagag atcgttcctg ctccgcattt cgccggatct
     4321 ctacgaagcc gtggcggcgt gggcggagga ggagcttcgc agcatgaacg gccagatcga
     4381 gttcctgctg cgtcgggtac tcctggaggc gggtcggctt ccccgccgac cgcctcccag
     4441 cgacggagcc gccggggcgt gaggcgccgc atggagcgcc agaggagaat gcgatgacta
     4501 ccacgacgcc ggagtgtccg gtgtgccggg tgcggatgga agaaggccat gtgctggacg
     4561 cggggaactc gaaccacctc ctgcgcgtga gctggaccga gggtcagccc gagaaggggc
     4621 tgatgggtta ccgggtcaag ggcaggcgcc gccttccgac catcacctgg cgctgccccc
     4681 gatgcgggtg gctcctgtgg ttcgccaggg aggagcagac gtgaccgcgg cgcggcgggg
     4741 aggagtcagg gggcggggag gcgcaagaac gagcgcagcc gttccagctc ctcctcgagc
     4801 gccaacggga aaaggcgatc ccgcggcttc gcgccgacga atccgacgtc ccaggtcagc
     4861 tcggcgccct tgacggagac gttcccccaa ccgatcacgc ggtcccgcca caacaggggc
     4921 agcgcgtagt aaccgcgctg ccgctttgcg gcgggcgtgt atgcctcgaa ccgataggtc
     4981 cagccccaca gaatctcgaa ccgcgagcga tcccagacga ccggatcgaa cggcgagagc
     5041 aggcgtacgc ggtcctcggg agcgcccgcc gcggccccgc cctcgggcca gtaccagtcc
     5101 acgccgtcga tgcacgcgtg gcgcagccgg cttacggccc gctggatcac gccaggcagg
     5161 tccgccttcc actgggggac ggcgtgtcgg aggtgtccga tgaggaaccg cagcctggaa
     5221 aaggatagcg gcgcgtagag gccgatcgcg gcgtccgcaa gggtatcgag ccgctcccgc
     5281 cgctccgcct cggttctcgg tcccggaccg tggcgctgct ccgcgtagat acggataccg
     5341 gattcgcgcc ggaccacccg gaccaccccg tggtacagca tggcctccag caggtgcgtg
     5401 gtggcgttgc tggcaccgcc aaaccagttt cggatcgtac cgatggcgaa tcgctcctcg
     5461 acctcctgcg gatgtacttc gccccgttcg cgcacgaacg ccagaacgcg gcgcgccagc
     5521 ctctgctgcg acgcatacca cgagcctccg ccctccgccg gcaccttgac ctgagggcgc
     5581 ggatgcatga gcgcctgcag ctcgctcgtg acgaagccat agttgacgaa gcgctcctcg
     5641 ttgacgccca gctcctcgta gcggcgctcc aggtcccccg cccggtaccc cttcacgcgg
     5701 tggaagagga tcaggtcctg ggcccgggct ggcgcgcgga tggggtccgc ctggacaaag
     5761 ccgagacggt ggagggcccg acgcagcgtg gtgggagcga agagggtccg ggtcacggca
     5821 agacgtcgta cctggtcgag gctggtcacc gatgcgccca cggcggtgaa ggtagccgat
     5881 tggccggacc ctcgccagag actcccctcg ctgccagctc gtggctcagc ggcgctcgat
     5941 cagcctgaac ttgccggttc gggcgtcgtt gtcgatccgg tccacgatct ccaaccggaa
     6001 gcgcagcgtc tcatgaagcc ccgatcccac caggatgtcg tgcatcttct cgtggatccg
     6061 atcgcggacg ggcgctccgt cgtgccgaac cttggcgcgc atgaggagga ggtccggcgg
     6121 gtggtgctcg tactgaaact tctcaagccc gggaacgaag aactctccga gcacgtgggg
     6181 gctgagcttc cgctcctgtc cgttgacaac gaaggagagg ctctcctccg atctaccgga
     6241 gagggcccgg atccgttgga aggggtggcc gtcggctcgc gaatcgtcgc cgcgggtcag
     6301 aaggtcactc atctcgtacc ggatgagggg ctgcgtccgg ttgtagaggt tggtgaggac
     6361 cagcctgccg gtttccccgg gcccgacctc cttcaggcgc tcatccacga tctcgaacac
     6421 gtgccagtcg tcgaacaggg acatgtcttc cccgccgttc cgctgcaccc ccatgcagat
     6481 cgactccgag cttgcgtaca ggttgatcgg ctcgattccg aacgcctcct ggacgtggct
     6541 ccgcaccaag tccgtcaacg ggtctcccga acagacaatc gcccgggggg aaatccgtag
     6601 ccggccgtga agttgctgga tggccaggag gtgggcgccg gaggcgtacc cgttgatcat
     6661 gtccggctga aacgcgttca gctcagcgac gatctgctcg atcggggcct ggatggacag
     6721 gggccgaaac tcgtagagaa accggggcgc ttcccggctg agggtgatgc ccgcatagtg
     6781 cccgtccgtg gcgccgacaa aggcaaggcg ggtcctgtga agcgggcgca cggagctgcg
     6841 tcccacccgg gagatggtca gggcccccag gatggaccac tcctctggcc cgtacacgaa
     6901 gagcccaacc gtgccgctgg accccgagga gtgaacgacc gcgaaccggt tcaggtatct
     6961 gcggccccga gtcgtcggat ccgcgacgaa ccgctccacc ccctccctgc ggagcgccgg
     7021 gtcacagctc agcgaatcga agctctccat cacctgactc ttggtgatgg tgggaagatc
     7081 ccgcagcggg atggcgtcca ggtcaccacc ccggatccct gccgaatggt agtggcgccg
     7141 gtagaactcg gagtggagat aggcgtgacg gagaagactt cgaaaccgcc gctcctgtcg
     7201 gagcagggtg gcctgccggc ttctattcag ctccagggag agatgcgtca gggatcggcc
     7261 gagaagaaag gtattcatcc ggtccgaaag atgctcattc gagtggcgct ggacaacctc
     7321 gccacggcaa tccgcgggca cgttgagccc caagggggag gtcgtctcca gaggtccccg
     7381 cgttgtcatt cgtcggcagg acggctatag tcctggcccg tgaacatcct gtctctcgac
     7441 ggcgtgagca agaccctggt ggatacgccg ctgttcgagc aggtgagcct ggggatcgac
     7501 gccggcgaca agataggatt catcggaaga aacggagcag gcaagtccac gttcctgaag
     7561 atcctcaccg gcgagcttgc cgcggatacc ggtagcgtgg cgcgaaaccg cacgttgacc
     7621 ctgagcgtcc tgccgcagcg tccgctcttc cttcccggaa cctccctgga ggagtattgc
     7681 ttccaggaga acgccgacca ggcggcgcct ggctccgaag aacacgccgc cgtcgcggac
     7741 gcctaccggt cgctctgccg tgagctcggg ctggccgacc cggctgcgcg catggacgct
     7801 ctctcgggag gcatgatccg caaagcctcg atcgcccgat gtcttgctcg aggcgccaac
     7861 ttcctcctcc tggacgagcc caccaatcac ctggacctgg acacgatcga atggctggaa
     7921 gaccggctgc gcggcgtctc ctttggattc atcctggtca cgcacgaccg ctacttcctg
     7981 gacggcgttt gcacggcgat catggagatc gacgaccgcg cggtccgcaa gtacgctggc
     8041 aattactccg tgtacctgga gcgaagggcg gagcgcgagg aagtgcgcc
//
LOCUS       DASRZS010000608         7493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3040492, whole genome shotgun sequence.
ACCESSION   DASRZS010000608 DASRZS010000000
VERSION     DASRZS010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3040492"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFH83_14820"
     CDS             complement(<1..186)
                     /locus_tag="VFH83_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487698.1"
                     /translation="MVYEIRIQETLSQDRSEWFEGMEMRVESEGQTILRGPIADQAAL
                     HGLLARLRDLNLTLLSVN"
     gene            complement(271..3567)
                     /locus_tag="VFH83_14825"
     CDS             complement(271..3567)
                     /locus_tag="VFH83_14825"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET6487699.1"
                     /translation="MASLGLYWLGPPRIEVEGRLIRLETRKVTALLAVLSVDRRAHAR
                     EHLAALLWPEYDATRAPANLRRALASLQASLGPGRLTSDREALELVMTTDVSVDTEEV
                     SSAIRRARAHHPDSEGELCPACARELERASELYRGDFLEGLNLKDCPDFDSWQTQKRD
                     ELSQATGWALERLADSSARAGRWAEAVDRARRWLALDPVHEAAHRTLMFLYARAGNPS
                     AALRQFQECERLLRQELGQEPSEATRSLHARILERKLEPARDPGAAPPASAAAAQAQK
                     PDATLAQKPPPGAASTRLRPPPIRPALVERARLLALLDQGASRPVTLVSAPAGFGKTT
                     LLAEWARGCGLPVAWLSLEEADSDPSRLAAGLASSLGRLDPRIGVEALQMLGSLQAAP
                     LPSVVASILSDLGAYPDSRVLVLDDYHLVQRREAEDLVADFAQHLPEGLHLFIATRAD
                     PAMPLARMRARAQLAEVRADDLRFRPEEAAAFLHQVMGLALTEEDIALLEQRTEGWAA
                     GLQMAALSLQRRQDPGAFIRSFGGSHRYIMDYLVGEVLEGQPADIQRFLLTTSVLDRF
                     CSELCDQVTERTGSQETLEGLDRANLFLVPLDDERRWYRYHHLFADLLRHRLRQQRAA
                     GEIDEMHLRAARWLEGSFDLEGAMRHYLAGRHYGEAIRLIVEKDTVILTRGGLGTLLG
                     WIAQIPAEEIDRSAQGSATVGTTFGWAGRPADAERHFARVDALLADAPDTGEAGAIKG
                     QAAVMRAFVADVAGDMGRAIELARRADTLLPRQASMARSLVPYILGRAYRYQGDLEKA
                     AAYTREQIALAKAANNLWSLSGALHELVILCRIRGTLREADRALDEFEAIPREATANG
                     PIAKVIADRAEIQREHGDLAAARSTMRTALEAVTRWGLPSDRYFCHLFGIRIGLSAGQ
                     LDLAEADLDAAEGLARTSLVYASMFPLLEAERVRVLLARGRLPEALAWLGSYQYPIES
                     NPVNGEVVSMARARVLLAAGRSAEASEALRRLAESAEAGGRFGRLVEILALETLALSK
                     ITGHRISGRENATRECLGRALAIAQPEGYVRVFLDEGEPMLDSLRCHLDRPEGIPPQI
                     LEFAQRLTIS"
     gene            complement(3577..5157)
                     /locus_tag="VFH83_14830"
     CDS             complement(3577..5157)
                     /locus_tag="VFH83_14830"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HET6487700.1"
                     /translation="MPKCDLSMCRPGIAALLVASGLLAACQSAPQPSTFQPANPRFRV
                     AVAEIHQETDSFSPVATTMADFEAAGVWRGAEVIERSLGKKTAIDGFLRAVKDMGRGE
                     VEVVPLLRATATSGGPVERAVYEQFKTDVVEGLRGAGRIDAVYLPLHGAMGVEGVRDP
                     EGDLLEAVRSVVGTEIPIGVSFDLHANLTKRRSELATFIVGYKTNPHRDFFQTGYDST
                     RILLETLRGQVKPVMVVRKMRLLKGGGMDIDFLPPMASIFSHMKSLERAKGVLSVSTF
                     MVHIWLDDPELGWSTVAITNADPALAARTADDLADRCWAVRAVPHEKGLSAEEAVAAA
                     KASGLARFFGTVVACDASDAVGAGAPGDSTLILQALLDQANDLVSYVPIRDPQAVDQL
                     WTSPEGQTVTATVGGRFGPQWSRPVEVTGTLLRKATGEAGRTVVLRSGGIHVILTTLP
                     TAAYTPSFFTGLGLSLWRADLVVVKNLFPFRYTYALYNRKTLEVITPGASDVDVFRLP
                     YSLVPRPIYPLDPIESWR"
     gene            5316..5762
                     /locus_tag="VFH83_14835"
     CDS             5316..5762
                     /locus_tag="VFH83_14835"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6487701.1"
                     /translation="MARLTIGVICALALTAVTLGWSDAQPPGAKAASPADALYADPDN
                     LQNLISQRPEPFYIVDVRTADEYETAHIPTAINIPWDTIAEHPPTPYTSTLIIVYCAS
                     GVRSARAKAALDKLGYTRVVDFGAISRWKGQTLKGEDPGECPCKTL"
     gene            complement(5719..6669)
                     /locus_tag="VFH83_14840"
     CDS             complement(5719..6669)
                     /locus_tag="VFH83_14840"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6487702.1"
                     /translation="MRIARASTDQTGYLYARPFAGLPALTHCGEIHSIGESSVALHAH
                     EGFEFAYLVSGTAWWQAGGSDFQQNPGELFFALPRQPHGTAHRAHPACHKLLLGLRVV
                     DLHGGPVAARLLHSLFLQGRQVLRGAQQTESLIRGLILQVVARRAGGEDVCAGYLRAF
                     LGLLTQAAATGVRPSSNADGRLCDAVRNALSFMSRELHRHVKLEEMAEASGLGVSQLC
                     FHFRREVGMTPAAYHLQMRLGAAREALLVPHTSITHVAMEFGFSSSQHFAMDFRRTFG
                     TTPLAWRRNGTDGSKGDGDGTSREPSVRGSYRDTRPDLPL"
     gene            6886..>7493
                     /locus_tag="VFH83_14845"
     CDS             6886..>7493
                     /locus_tag="VFH83_14845"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6487703.1"
                     /translation="MLRRRWFIVLLALVLAAGMAMPAFSQKVTITWWGWITPDWVATY
                     RAYEKLNPNVTIKEALVSEEWASTAEKFLAAVAAGIAPDASVQNSHEFPHFASQGVFL
                     DLTPYMTKDAMKAADWFTPQWNGAAFKGKRFGMPGVTDTRVLYYNKKLMRDAGLDPNK
                     PPATGKDLETMAAKLNKKDSAGKLVQYGFIPTIQTGMPAAGNA"
BASE COUNT         1250 a         2565 c         2522 g         1156 t
ORIGIN      
        1 attgaccgaa aggagggtca ggttgaggtc acgcaggcga gcaagcagtc catggagggc
       61 ggcctggtcg gcgatggggc cgcggaggat cgtttgtccc tccgactcca cgcgcatctc
      121 catgccctcg aaccactcgg accggtcctg gctcagggtc tcctggatcc ggatctcgta
      181 taccaaacac cgcgctccca tccttttgtc cctccgtccg atctgtcatg aacctacggg
      241 acggcgggta cgggagcgtt ccgagcggcg ttacgagatc gttaaccttt gggcgaactc
      301 caggatctga ggtgggatcc cttccgggcg atccaggtgg catcggagtg agtcgagcat
      361 gggctctccc tcatcgagga agacccgaac gtatccctcc ggttgggcga tggcgagtgc
      421 gcggccaagg cattccctcg tggcgttctc ccggccggag atccggtggc cggtgatctt
      481 cgagagtgcc agcgtctcca gcgccaggat ctccacgagc cttccgaagc ggcccccggc
      541 ctccgctgat tccgcgagcc tccgcagcgc ctctgacgcc tccgcagatc ggcccgcggc
      601 aaggagcacc cgggcgcggg ccatggacac gacttctccg ttcacgggat tgctctcgat
      661 gggatactgg tagctcccga gccacgccag agcctccggc aggcgtccgc gcgccaggag
      721 cacgcgcact cgctccgctt ccaggagcgg gaacatcgag gcgtagacca aggacgtcct
      781 ggccaggcct tcggccgcgt ccaggtccgc ttcggccaga tcgagctggc cggcggaaag
      841 gccgatccgg atcccaaaga ggtggcagaa gtaccggtcc gacggaagcc cccatcgggt
      901 gaccgcctcc agcgccgtcc gcatggtgct ccgcgcggcc gccaggtccc catgctccct
      961 ctggatctcg gctcgatcgg cgatgacctt ggcgatggga ccgttggccg tggcctcccg
     1021 agggatcgcc tcgaactcgt ccagcgcgcg gtccgcctct cggagcgtgc ctcgaatccg
     1081 acagaggatc accagctcgt gcagcgcgcc ggagagcgac cagaggttgt tcgcggcctt
     1141 ggccagcgcg atctgttctc gggtatacgc tgcggctttc tccagatcgc cctggtagcg
     1201 gtacgccctt cccaggatgt agggcaccag ggaccgagcc atggaggcct gtcggggaag
     1261 cagcgtgtcc gctcggcgag ccagctcgat ggcgcggccc atgtcgccgg caacatcggc
     1321 gacaaacgcg cgcatcacgg ccgcctgccc cttgatggca ccggcctcgc cggtgtccgg
     1381 agcgtcagcc aggagcgcat ccacccgggc aaaatgccgc tccgcatccg cgggtctgcc
     1441 ggcccagccg aaggtcgtcc cgacggtggc gctcccctgg gcgctgcggt cgatctcctc
     1501 cgcggggatc tgggcgatcc agccgaggag cgtgcccagg ccgccgcggg tcaggatgac
     1561 cgtgtccttc tccacgatca ggcggattgc ctctccgtag tggcggccgg ccaggtaatg
     1621 cctcatggct ccttcgagat cgaaggaacc ctcgagccac cgcgcggccc gcaggtgcat
     1681 ctcgtcgatc tctcccgcgg cccgctgctg gcgcagccgg tgtcgaagca ggtcggcaaa
     1741 caggtgatgg tatcggtacc accggcgctc gtcgtccagg gggaccagga agaggttggc
     1801 ccgatccaat ccctcgagcg tttcctggga tcccgtccgt tcggtcacct ggtcgcagag
     1861 ctccgagcag aaccggtcca ggaccgacgt ggtgagcagg aaccgctgga tatcggcggg
     1921 ctgaccctcc aggacctcgc ccaccaggta gtccatgata tagcggtggc tcccgccgaa
     1981 gcttcggatg aaagcgcccg gatcctgccg gcgctgcagg gacagcgcag ccatctgcag
     2041 gccggcggcc cacccctccg tacgctgctc cagcagggcg atgtcctcct ccgtgagcgc
     2101 caggcccatg acctggtgca ggaacgccgc ggcttcctcc ggacggaacc tgaggtcatc
     2161 agcgcggacc tcggcgagct gcgctctggc gcgcatgcgc gccaggggca tggccggatc
     2221 ggcgcgggtg gcgatgaaga ggtgcaggcc ctccggcagg tgctgggcga agtcggccac
     2281 gaggtcctcc gcctcccgtc gctgcaccag gtggtagtcg tcgagcacca ggacgcggct
     2341 gtcgggatag gcgcccaagt ccgagaggat ggaggcaacg acggagggca ggggggccgc
     2401 ctggagggag ccgagcatct gcagggcctc gaccccgatc cgcggatcca ggcgccccag
     2461 ggaggacgcc agcccggccg ccagccgcga cgggtcgctg tccgcctcct cgagagacag
     2521 ccaggccacc ggcagaccgc atccccgtgc ccactccgcc aggagggttg tcttgccgaa
     2581 acccgccggc gcggagacca gggtcacggg tcgtgaagct ccctggtcca gaagcgcgag
     2641 aagccgggca cgctcgacca gggccggccg gatgggcggt gggcggaggc gggtggaagc
     2701 cgcccccggg ggcggcttct gcgccagggt cgcatcgggc ttctgcgcct gcgctgctgc
     2761 cgctgacgct ggaggcgcgg cacccgggtc cctcgcgggc tccagcttgc gctccaggat
     2821 gcgcgcgtgc agagaccgtg tggcctccga cggctcctgc ccaagctcct ggcgcagaag
     2881 ccgctcgcac tcctggaact gccgcagcgc ggcgctgggg tttccggcgc gagcgtacag
     2941 gaacatgagc gtccgatggg ccgcctcgtg cactggatcc aacgcaagcc agcgccgcgc
     3001 acggtccacc gcctccgccc accgcccggc ccgagcgctt gagtccgcca ggcgctcgag
     3061 cgcccagccc gtagcctggg acagctcatc gcgcttctgc gtctgccagg agtcgaagtc
     3121 cgggcagtcc ttcaggttga gcccttccag gaagtccccg cggtagagct cggaggccct
     3181 ctccagctcc cgcgcgcagg ccgggcacaa ctcgccttcc gaatccggat ggtgggcccg
     3241 agcgcggcgg atcgcgctgg agacctcctc ggtatccacg gaaacgtccg tggtcatcac
     3301 cagctccagg gcttcccgat ccgacgtgag ccggccgggg cccaggctcg cctgcaggga
     3361 ggcgagggcc cgccgcaggt ttgccggagc gcgcgtggcg tcgtactccg gccagagcag
     3421 ggccgccagg tgctcgcggg cgtgagccct gcggtccacc gaaaggaccg ccagcagcgc
     3481 cgttaccttc cgagtctcca gtcgtatgag ccggccctcc acctctatcc gcggcgggcc
     3541 aagccaatag agacccagcg atgccaccgg atcctcctat cgccagctct cgatcgggtc
     3601 gagggggtag atcgggcgcg ggaccagcga gtaggggagc cgaaacacat ccacgtccga
     3661 ggcgccgggc gtgatcacct ccagggtctt tcgattgtag agggcatagg tgtagcgaaa
     3721 cgggaagagg ttcttgacca cgacgagatc cgccctccag aggctcaatc ccaggccggt
     3781 gaagaaggag ggggtgtacg cggccgtggg gagcgtcgtc aagatcacgt ggatgccgcc
     3841 ggagcggagg acgaccgttc gtccggcttc cccggtggct tttcgcagga gcgtgccggt
     3901 gacctccacg ggacggctcc actggggccc aaacctcccg cccacggtcg ccgtcacggt
     3961 ctgcccctcc ggggaagtcc agagctggtc cacggcctgg gggtcgcgga tggggacgta
     4021 ggacacgagg tcgttggcct gatccaggag tgcctggagg atcagggtgc tgtcccccgg
     4081 cgccccggca cccaccgcgt ccgacgcatc gcacgctact accgtgccga agaaccgagc
     4141 caggccggac gcctttgccg ctgccaccgc ctcctccgcg gacaggccct tctcgtgggg
     4201 gaccgcccgc accgcccaac accggtccgc cagatcgtcc gcggtccgag cggccagggc
     4261 cggatccgcg ttcgtgatgg caacggtgga ccaccccagc tcgggatcgt cgagccagat
     4321 atggaccatg aaggtggaga cggagaggac tcctttggcg cgctccaggc tcttcatgtg
     4381 gctgaagatg gacgccatgg gcggaaggaa gtcgatgtcc attccacctc ccttcaggag
     4441 ccgcatcttg cgcactacca tgaccggctt cacctggccg cgcagggtct ccaggaggat
     4501 ccgcgtggag tcgtagcctg tctggaagaa gtcacggtgt gggttggtct tgtagccgac
     4561 gatgaaggtt gccagctcgg accgacgctt cgtgaggttc gcgtgaaggt cgaaggacac
     4621 cccgatgggg atctccgtcc ccaccacgga gcggaccgcc tccagcaggt ctccctcggg
     4681 atcccgaacc ccttcgaccc ccattgcccc gtgcaggggc aggtacaccg catcgatcct
     4741 tcccgcgccg cgaagcccct ccacgacgtc ggtcttgaac tgctcgtaga ccgcgcgttc
     4801 gacgggaccg ccggaggttg ccgtagcgcg aagcaggggc accacctcca cctctcccct
     4861 gcccatgtcc ttcaccgccc gcaggaaccc atcgatcgcg gtttttttcc ccaggcttcg
     4921 ctcgatgacc tcggcgccgc gccagactcc agccgcttcg aagtccgcca tcgtcgtggc
     4981 aaccggcgag aaggagtccg tttcctggtg gatctccgcc acggccacgc ggaacctcgg
     5041 gttggccggc tgaaaggtcg aaggctgggg cgcggactgg caggctgcca gcaggccgga
     5101 ggcgaccagg agcgcagcaa tacccgggcg gcacatcgag agatcgcatt tgggcatgtc
     5161 ctgactttag tcccggtggg gtacggaagc aagcggcggg agaccggacc cccgccgccc
     5221 gacgggctcc tccttgacca ctctctgccc gcagctacaa tgccgcccct gaggccgcgg
     5281 tcccggaccc ggcccgctcg agggaggacg ttgccgtggc tcgcctgacg atcggggtta
     5341 tctgtgcgct cgccctaacg gcggtgactc tgggctggag tgacgcccag cctccgggcg
     5401 cgaaggcggc gtcacccgcg gacgccctgt acgcggatcc cgacaatctc cagaacctca
     5461 tttcccagag accggaaccc ttctatatcg tggacgttcg gaccgcggat gagtatgaga
     5521 ccgcccacat tccgaccgcc atcaacatcc cctgggacac gatcgccgaa cacccgccca
     5581 cgccctacac ctcgacgctg atcatcgtgt actgcgcttc cggggtccga tccgcccgag
     5641 ccaaggccgc gctggacaag ctgggctaca cgagggtcgt ggactttggg gcgatctccc
     5701 gttggaaggg ccagaccctc aaaggggaag atccgggcga gtgtccctgt aagaccctct
     5761 gacggagggc tcccggctcg tcccgtcgcc gtcccccttg gacccgtctg tcccgttccg
     5821 gcgccaggcc aggggggtcg tgccgaacgt cctgcggaaa tccatcgcga agtgctggga
     5881 ggagctgaag ccgaactcca tcgccacatg ggtgatgctc gtgtgcggca ccaggagcgc
     5941 ctcccgcgcg gctcccagcc gcatctgcag atgataggcg gcgggcgtca tccccacctc
     6001 ccggcggaag tggaagcaga gctgggagac gcccagtccg ctggcctccg ccatctcttc
     6061 cagcttcacg tggcggtgga gctcgcgact catgaatgac agggcgttcc gcacggcatc
     6121 gcaaagcctc ccgtcggcgt tggacgaggg ccgcacgccc gtggccgccg cctgcgtgag
     6181 cagtcccaga aacgcccgca ggtagccggc gcacacatcc tcgccgccgg cgcgccgagc
     6241 gacgacctgc aggatcaggc cgcgaatcag ggactccgtc tgctgcgccc cccgaaggac
     6301 ctgacggccc tggagaaaca gggagtggag gagcctcgcg gccacgggcc ctccgtgcaa
     6361 gtccaccacc cgaagcccga gcagcagctt gtggcacgcg ggatgggcgc ggtgcgccgt
     6421 tccgtgtggc tggcgaggca gcgcgaagaa gagctctccc gggttctgct ggaagtccga
     6481 tccgccggcc tgccaccagg cagtcccgga aaccaggtat gcgaactcga agccctcgtg
     6541 ggcatgaagg gccaccgagg actccccgat agagtggatc tcgccgcagt gggtgagggc
     6601 cgggagcccc gcaaaggggc gcgcgtacag gtagcccgtc tggtcggtgg aagcccgcgc
     6661 gatccgcatg gcgggaggga gtatagcacg gatccgaagg agagaccgca agattctgta
     6721 caattcccgc aggaaactgg cagacaccgg gcacgaggcg tgctagcatg cggtcatagt
     6781 tcgcagagga aaggagggtc tctccctgga atctggggtc gttcgcttgc gaacgggcga
     6841 cgaccgtcaa ctctcaaata ctgttaacgc aatggaggtg agactgtgct gaggaggaga
     6901 tggtttatcg ttctgctggc gcttgtcctt gccgcgggca tggcaatgcc ggcgttctct
     6961 cagaaggtga cgatcacctg gtgggggtgg atcactccgg actgggttgc cacctatcgg
     7021 gcgtacgaga agctcaaccc caacgtgacc atcaaagaag cgctggtctc ggaggagtgg
     7081 gcgtccacgg cggagaagtt cctggcggct gtcgccgcag ggatcgcgcc cgatgcgtcg
     7141 gtgcagaact cccacgagtt ccctcacttc gcttcccagg gcgtattcct ggatctgact
     7201 ccctacatga ccaaggacgc catgaaggcc gcggactggt tcaccccgca gtggaacggc
     7261 gcggccttca agggcaagcg attcggtatg ccgggggtca cggatacccg cgtgctgtac
     7321 tacaacaaga agctgatgcg ggacgcgggc ctggatccca acaagccccc cgccaccggc
     7381 aaggaccttg agaccatggc ggccaagctg aacaagaagg acagcgccgg caagctggtc
     7441 cagtacggtt tcatcccgac catccagacg ggtatgcccg cggcgggcaa cgc
//
LOCUS       DASRZS010000609         3362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1123922, whole genome shotgun sequence.
ACCESSION   DASRZS010000609 DASRZS010000000
VERSION     DASRZS010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123922"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VFH83_14850"
     CDS             complement(<1..780)
                     /locus_tag="VFH83_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487704.1"
                     /translation="MTGLMQGLMYEIILGLYILSFSLGVVAVTVSLLANQRFSNNLFV
                     MVALVFVASLLLIMADFVGLRALVRPALFGGSIFALSLVFTTVGNALFAYSLIRLVFR
                     LVGTDAPSPLLIVLSTAALAVLGALDDLVPGPVTALTDQIGLSALGFTCIGVVAWRMD
                     RVGVVVHKSFLRYGLVACFAMTFLCLAQRVWQVLPHHPAGVSDLPITDLVYCLVASGL
                     LLIWAFRYLFKTDLGGDPGIDPAAATEFGISPRELEIVSLMA"
     gene            complement(946..1917)
                     /locus_tag="VFH83_14855"
     CDS             complement(946..1917)
                     /locus_tag="VFH83_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HET6487705.1"
                     /translation="MAKSELIGGTLWESYSNKVNDRMLNPRHLGEITEEQAARMGGKL
                     VVADWGAEVCGDAIRAFWVVNPKTSRILDARFKTFGCGTAIASSDMMCEMIIGKTVDQ
                     AMGITNLDVERALRDDERTAAIPPQKMHCSVMAYDVIRKAASEYKGVDMDSLEEEEIV
                     CQCARVSLRTVREVIRINDLKSVEEITHYTKAGAFCKSCIRPGGHESRKRYLVDILQE
                     VRAEMDREKTSAATDRGPKSFASMSLIQKHRAVEKVLDEQVRPTLLKDGGNLEVVDMK
                     VTDGFTELYINYLGACKGCPSASIGTLSYIEQFLQKELDPSIRVLPL"
     gene            complement(1904..3097)
                     /locus_tag="VFH83_14860"
     CDS             complement(1904..3097)
                     /locus_tag="VFH83_14860"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002957042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifS family cysteine desulfurase"
                     /protein_id="HET6487706.1"
                     /translation="MDRIYLDNNATTIVDPRVKEAMEPFWSQLYGNPNSLHGFGTEVH
                     PYMRLAFDRLYAGVNARDDDDILITSCATESNNAVLKSAYYQLIHTGRRSQIVTTQVE
                     HPAVSNTCRFLQGLGAEVTYLPVNADGIVDAAILRDHIDADRTALVSIMWANNETGLV
                     FPIRELAELCRERGILFHTDAVQAIGKEPVDVQQAPVDYLSFSAHKFHGPKGVGGLYV
                     RKGAPLIPLLHGGEQMNGKRGGTVNVAFLVGMGLAMELAAEGLRHERTEVRRLRDRLE
                     DALLGMPGITVIGRRELRTPNTILLSVQAVEGEAMIWDLNRQGIAASTGSACASESLE
                     PNPTFVAMCLDNELAHTGVRLSLSRFTTEAEIDVAITAIRQSVDRLRAISPSAGKRED
                     IRGKV"
     gene            complement(3163..>3362)
                     /locus_tag="VFH83_14865"
     CDS             complement(3163..>3362)
                     /locus_tag="VFH83_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487707.1"
                     /translation="IRFVHVDNPVYQFTPFNFTTGQILNVLMITGAVVFLYVLRRRAR
                     LDAAAPASAPPSARKLRKKLK"
BASE COUNT          601 a         1083 c         1086 g          592 t
ORIGIN      
        1 cgccatgagg ctgacgatct cgagctctcg aggcgaaatc ccgaactccg ttgccgcggc
       61 gggatcgata cccggatctc cgcccagatc cgtcttgaag agataacgga acgcccagat
      121 caggagaagc ccggacgcca ccaggcaata gacgaggtcg gtgatgggaa ggtcactcac
      181 accggccgga tggtgcggaa ggacctgcca tacgcgctgc gccaggcaca ggaaggtcat
      241 tgcgaagcag gcgaccagac cgtagcgcag gaacgacttg tgcacgacga ccccgactcg
      301 gtccattctc caggccacca cccctataca cgtgaacccc agggccgaca atccgatctg
      361 atccgtcagg gcggtgaccg gacccggtac gaggtcgtcc aatgccccca agacggcgag
      421 cgccgcggtg ctgagcacga tgaggagcgg gcttggggcg tccgttccca ccaggcggaa
      481 caccaggcgg atcagggagt aggcgaaaag cgcattcccg acggtcgtga aaaccaggga
      541 cagcgcaaag atcgacccac cgaacagcgc tggacggacc agggcgcgaa gcccaacgaa
      601 gtccgccatg atcagcaaga gactcgcgac aaaaaccagc gcgaccatga cgaaaaggtt
      661 gttgctgaat cgctggttgg ccaggagact tacggtgacg gccacgaccc cgagggaaaa
      721 cgagagaatg tacaagccaa ggatgatctc atacatcaag ccctgcatca gcccggtcat
      781 cccctctcgt ggccgctgtt cgaacgaacc gtgcacctct tcgccgaaag tatcaatgtg
      841 acattagggc gagaggattc tactcgccgg caggaatcat aggctgttcc atgacgggcg
      901 tcaatcttcc agcccttggg gctcgagccc actgcatcga gagaatcaga gcggcaggac
      961 ccggatgctc gggtcgagct ccttctgaag gaactgctcg atgtaggaga gagttccgat
     1021 ggatgcgctg gggcagcctt tgcaggcgcc gaggtagttg atgtacagct cggtgaaacc
     1081 gtccgtcacc ttcatgtcga ccacctcgag gtttccgccg tccttcagaa gcgtgggtcg
     1141 tacctgctcg tccaggacct tctccacggc gcgatgcttc tggatgaggc tcatcgacgc
     1201 gaagctcttg gggccgcgat ccgtggcggc ggaagtcttc tcccggtcca tctccgcccg
     1261 cacctcctgg aggatgtcca ccaggtaccg ctttcgggat tcgtgtccgc cgggccggat
     1321 gcaggacttg cagaacgcgc cggctttcgt gtagtgggtg atctcctcca cgctcttgag
     1381 gtcgttgatc cggatcacct cccgcaccgt gcgcaggctg acccgggcgc attggcagac
     1441 gatctcctct tcctccaggg aatccatgtc cacgcccttg tactccgagg ccgccttgcg
     1501 gatcacgtcg tacgccatga cgctgcagtg catcttctgc ggcgggatgg cggcggtccg
     1561 ctcgtcgtcc cgaagcgcgc gctccacgtc caggttggtg atgcccatgg cctggtccac
     1621 ggtcttgccg atgatcatct cgcacatcat gtcgctcgag gcgatggcgg tgccgcagcc
     1681 gaaggtcttg aaccgtgcat ccaggatgcg gctggtcttg gggttcacca cccagaaggc
     1741 gcggatggcg tcgccgcaga cctccgctcc ccagtccgcc actacgagct tccctcccat
     1801 ccgggccgcc tgctcctcgg tgatctcccc gaggtgtcgg gggttcagca tccgatcgtt
     1861 caccttgttg gagtagctct cccagagggt cccgccgatc agctcagact ttgccacgga
     1921 tgtcctctct ctttccggcg ctcggggaga tggctcgcag ccggtccacg gactggcgga
     1981 tggccgtgat ggccacgtcg atctccgcct cggtcgtgaa ccgcgacagg gaaaggcgca
     2041 cgccggtgtg ggccagctcg ttgtccagac acatcgccac gaaggttggg ttcggctcca
     2101 gggactcgga agcgcatgcg ctgccggtgg acgccgcgat gccctggcgg ttgaggtccc
     2161 agatcatcgc ctctccctcc acggcctgga cgctgaggag gatggtgttc ggcgtccgaa
     2221 gctccctccg tccgatcacg gtgatgcccg gcatccccag gagcgcgtcc tccagacggt
     2281 cgcggagccg acggacctcc gtccgctcat gccggagccc ctcggcggcc agctccatgg
     2341 ccagccccat gccgaccagg aacgccacgt tgacggtgcc gccccgcttg ccgttcatct
     2401 gctctccgcc gtggagcaga gggatcaggg gggcaccttt gcggacgtac agaccaccga
     2461 cgcccttggg gccgtggaac ttgtgggcgc tgaacgagag gtagtccacc ggcgcctgct
     2521 gcacgtccac cggctccttg ccgatggcct ggaccgcgtc ggtatggaac aggatcccgc
     2581 gctcgcggca cagctcggcc agctcgcgga tggggaacac caggcccgtc tcgttgttgg
     2641 cccacatgat ggagaccagg gccgtgcggt ccgcgtcgat gtggtccctg aggatcgcgg
     2701 catccacgat cccgtccgcg ttcaccggca ggtacgtcac ctcggcgccc agcccctgga
     2761 ggaaccgaca ggtattgctg accgccggat gctcgacctg ggtggtgacg atctgggagc
     2821 gtcgcccggt atggatgagc tggtagtaag ccgacttgag gaccgcgttg ttggactccg
     2881 tcgcgcagct cgtgatcagg atgtcgtcgt catcgcgcgc gttgaccccc gcgtacaggc
     2941 ggtcgaacgc cagccgcatg tacgggtgca cctcggtgcc gaatccgtgc agggagttgg
     3001 ggttgccgta gagctgggac cagaacggct ccatggcttc cttcacccga gggtccacga
     3061 tcgtcgtggc gttgttgtcc agatagatgc ggtccatggt ctccccacta tagcgaacgg
     3121 cggcggactt gagcaatcga ggccggggac acccggcggc cgctacttca gcttcttgcg
     3181 gagcttccgc gcgctgggcg gcgccgaggc cggggcggcg gcgtccagcc gggcccgccg
     3241 ccgcagcacg tagaggaaga cgacggcccc ggtgatcatc aggacgttga ggatctgacc
     3301 cgtggtgaag ttgaatggcg tgaactgata cactggattg tcgacgtgga cgaagcggat
     3361 gg
//
LOCUS       DASRZS010000610         6365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_342020, whole genome shotgun sequence.
ACCESSION   DASRZS010000610 DASRZS010000000
VERSION     DASRZS010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342020"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VFH83_14870"
     CDS             <1..877
                     /locus_tag="VFH83_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6487708.1"
                     /translation="VVGTRPTAEVDRAELVRMMVGQELSEPSPRPLRPGPVRLTIEGL
                     RAAGDRGGDALRGVDLVIHAGEIVGLAGVSGNGQRELAECLAGMRKGTGGCIRLESAD
                     ITSCTPNQRVEAGMAYVPEERMRDGAIREFTVQENIFLHDHSARRFARGIFLRFSRME
                     GHAAELVRDFAVKTPTLDTPLKNLSGGNIQKLIMARELSRRPKVLIAAQPTRGVDISS
                     TAYIHQRLLAQREAGTAILLISEDLEEITALSDRIAVMYEGRIVGVAGRGEATLEQIG
                     LMMAGVLQARARPRE"
     gene            947..2473
                     /locus_tag="VFH83_14875"
     CDS             947..2473
                     /locus_tag="VFH83_14875"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6487709.1"
                     /translation="MAGKNGFRIEQTDAYRQVTSGSSRRLFVALGTIAVLANAATVVI
                     KAIGASSEHLTYASIAVELGAVVVITTASYLLSRRFPSPYIAITGAMIALWVFQYVIY
                     GAPELFAVHYLAIALSVFYFDRKVSIYTLAVVLVSQTLLFILRPELIPPGAHSNLMIR
                     YLAYLWVGIGAMFGAGAARRLLTMAVQSQDLAEENFTRLKSVAKAVVDSIVVLDTQAK
                     EQDTVTAQLNEISLHQASSLEQISASLEHLSAGSQSINSVARSVHQELSVTTASVRDL
                     KAVNQSAQASSRQIATSIEEVNTDSESSARQIERTREMIATLSAKSEEMSEFVRIIND
                     IASEVNLLSLNAAIEAARAGEHGRGFSVVADEISKLAEATTRNSKEIARIITENQKLI
                     AGSDELITQSSAHIRSLAGSISEVSTRLRDVTKLIGDLDATIKTIENLNAKVSESSQI
                     IESSTDEQTNATGESSKTTHEISRTAQEIVGIAAKVAGSAKVINELTARLGNLTAGMV
                     "
     gene            2561..2986
                     /locus_tag="VFH83_14880"
     CDS             2561..2986
                     /locus_tag="VFH83_14880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET6487710.1"
                     /translation="MSTRSRYGLKIMYELAVRYGGDPVFLKDIARAHRISAKYLSKLV
                     IPLRGARLITSFRGAHGGYALTRDPGTISLLEIVQALEGEIATTRQPRKRADAEGGSI
                     HPADGVWTGLERTINEALGKVSLESLVNQAREIVLNYQI"
     gene            3019..4176
                     /locus_tag="VFH83_14885"
     CDS             3019..4176
                     /locus_tag="VFH83_14885"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6487711.1"
                     /translation="MDRRLHNKEMRMADRLAARQKWELLLSGGDPGPMVSPLCDDWSL
                     DVPYSWPYPEPDPFPPGHSKHTLSQQMAMAGLCGWEPTFLCTLDMPPRDAEVLPRTVT
                     SQEGGATRTTSEIRTPYGTLTSITEAQRTSRTLKPWIETEADFHRMSWVARKVADYDE
                     DAAIEQGRVLCAAVGDRGVLGTWFGPPIGYNLNRDAMFYHLADWPEAFEELHQATRAL
                     AFRQIETLRKAGFDYLFYCVDATEWISPDFFRRWILEDTRRLFARWHELGGFVLWHSC
                     GKVATFLAQGFYNDLAPDVLETLSEAPLGDLPSLRWARDRLDRKIITKGNIGLDILLG
                     GTEEEVRREVSRVRGETAGYRHVVGLSDDILKGTPLRNARALVEESRASIR"
     gene            4425..5480
                     /locus_tag="VFH83_14890"
     CDS             4425..5480
                     /locus_tag="VFH83_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HET6487712.1"
                     /translation="MKMRPATSIVALSLIVLLAAFGLGGCGLAQQLGLIAPNAPTGLA
                     ASWVTGSSTDIEVSWNSSVGATSYTLSRSSNGSSGPFKSVYTGASVNFTDTGLSAAVS
                     YWYEVVATSGAGSSANSDIVEVSSQTSAGSPVIALAAASLVFTATAGGADPAAQTVSV
                     TNTGAGTLSGLSATVSSSGNGWLTASLSATTAPATLTVTPSTGSLSAGTYTATVSVAS
                     SVSGVTSKSVGVTLTVSGPPAIGLSAATLTFTAMPGGADPSEQTVSVTNTGGGSLTGL
                     SVIVSPSGNGWLTASLSSASAPATLTVTPSTGSLAAGTYTATVSIASSVPGVVTQSVG
                     VTFTVSSDVGSDVITVQ"
     gene            5508..>6365
                     /locus_tag="VFH83_14895"
     CDS             5508..>6365
                     /locus_tag="VFH83_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487713.1"
                     /translation="MREIDHHSNTRTIRRDQELRKNLSLVLFALLVALLVGCTMPESP
                     LPAATATGGGSANGGVAAASSGSATSSGAANTAAGATAGTFKLTVPHLAPWVVKATAK
                     ASSSRAFVEADSGDFTIVDHNNDNATVGSGPLNGSTTVLAGTTAYTTSGVLPAGSYGV
                     TVNIYNANDGEGAVVSGEADFTVIAGRTIPVTVTCFPVNSTTVEMPAQNTESVALSPG
                     GYESWFWVPSGYATQTGFEVTASNYGESFGTVYYFVFYADGTYTGSFSTANPDNPSTF
                     VDVNPSEGEA"
BASE COUNT         1153 a         2057 c         2065 g         1090 t
ORIGIN      
        1 cgtggtcgga acccgtccca cggcggaggt cgaccgcgcg gagctggtgc ggatgatggt
       61 ggggcaggag ctttccgagc cttcccccag gcccctgcga cccggcccgg tgcggctgac
      121 catcgagggg ttgagggccg ccggggaccg gggcggcgac gcgcttcgcg gagtggatct
      181 ggtgatccac gcgggagaga tcgtgggtct ggcgggcgtg tccggcaacg gccagcgcga
      241 gcttgccgag tgcctggccg gcatgcgcaa ggggaccggc gggtgcatac ggctggagtc
      301 ggcggacatc acgtcctgca ccccgaatca gcgggtcgag gccggcatgg cctacgtccc
      361 cgaggaacgg atgcgggacg gcgcgatccg ggagtttacg gtccaggaga acatcttcct
      421 tcatgaccat tcggcgcggc gtttcgcccg ggggatcttc ctccggttct ctcgcatgga
      481 agggcacgcg gcggagctgg tccgggactt cgcggtcaag acgcccaccc tggacacgcc
      541 gctgaagaac ctctccggcg gaaacatcca gaagctcatc atggcgcggg agctgtcgcg
      601 ccgtcccaag gtcctgattg ccgctcaacc cacgcgaggc gtcgacatca gctccaccgc
      661 ctacatccac cagcggctgc tggcccagcg agaggccgga acggcgatcc tgctgatctc
      721 cgaggacctc gaggagatca ccgcgctctc ggaccggatc gcggtgatgt acgagggacg
      781 cattgttggc gtggcgggcc ggggggaggc cacgctcgaa cagatcgggc tcatgatggc
      841 aggcgttctg caggccaggg cccggccgag agaatagcgg cgcctgcccg tcctggccaa
      901 cattcctcgg tgtgcaccgc cgagccgcgt gttagactaa aggagtatgg cgggcaaaaa
      961 cggattcaga atcgagcaga ccgacgccta ccgacaggtc acgtccggca gcagtcgcag
     1021 gcttttcgtc gccctgggca cgatcgcggt cctggccaac gcggccacgg tggtcatcaa
     1081 ggcgattggc gcgtcgtccg agcaccttac gtacgcctcc attgccgttg agctgggcgc
     1141 cgtggtggtg atcacgacgg catcctatct gctgagtcgg cgcttcccgt cgccctatat
     1201 cgcgatcacc ggagccatga tcgcgctctg ggtgttccag tacgtcatct acggcgcccc
     1261 ggagcttttc gcggtgcact acctggccat cgccctgtcc gtcttctact ttgaccgaaa
     1321 ggtctcgatc tacaccctgg ctgtcgtcct cgtctcccag accctgctgt tcatcctccg
     1381 acccgagctg attccgcccg gcgcccacag caacctcatg atccggtacc ttgcctacct
     1441 ctgggtcggc atcggcgcca tgttcggagc gggtgccgcc cgcaggctgc tgaccatggc
     1501 ggtgcagagc caggacctgg cggaagagaa cttcaccaga ctgaagagcg tggccaaagc
     1561 ggtcgtcgac tccatcgtcg tgctggacac ccaggcaaag gagcaggaca cggtcaccgc
     1621 gcagctcaac gagatatccc tgcaccaggc gtcctccctg gagcagatca gcgcctccct
     1681 ggaacacctc tccgccgggt cgcagtccat caactccgtc gcccgatcgg tccaccagga
     1741 gctttcggtc accaccgcgt cggtgcggga cctcaaggcc gtgaaccaaa gcgcccaggc
     1801 gagctcccgg cagatcgcca cttccatcga ggaagtgaac accgattccg agagctcggc
     1861 ccggcagatt gaacggacgc gggagatgat cgctaccctc tccgccaaga gcgaggagat
     1921 gtcggagttc gtccgcatca tcaacgacat cgccagcgag gtgaatctcc tcagcctgaa
     1981 cgccgccatc gaggccgcgc gggcgggcga acacgggagg gggttctccg tggtcgccga
     2041 cgagatctcg aagctcgcgg aggctaccac ccggaactcc aaagagatcg ccaggatcat
     2101 caccgagaac cagaagctca tcgcgggctc ggatgagctc atcacgcagt cctccgccca
     2161 tatccgcagc ctcgcgggct ccatctccga ggtgagcacc cggctgcggg acgtcacgaa
     2221 gctgatcggt gatctcgacg ccaccatcaa gaccatcgag aacctgaacg ccaaggtgtc
     2281 cgaatcgagt cagatcatcg agagctccac cgacgaacag acgaacgcca ccggggagtc
     2341 cagcaagacc acccatgaga tctcgaggac cgcgcaggag atcgtgggga ttgccgcgaa
     2401 agtggccggc tccgccaagg tgatcaacga gctcaccgcc aggctgggga acctcaccgc
     2461 gggcatggtc tagcgcgtga ccgttgatcc cttgcagacc actgaattga cttggcgctc
     2521 cctcgctcac tatgcttgcg gcgggtaggc ggacatgctc gtgtctacac ggagccggta
     2581 cggcctgaag atcatgtacg agctggcggt gaggtatgga ggcgacccgg tcttcctcaa
     2641 ggatatcgcc cgggcgcatc gtatctccgc gaaatacctg agcaagctcg tgatccccct
     2701 gcggggagcg cgtctcatca cctcgtttcg gggagcccat ggcggctacg ccctgacccg
     2761 agacccgggc actataagcc tcctggagat cgtccaggcg ctcgaagggg agattgccac
     2821 cacgcggcag ccgcggaaga gagcggatgc cgaaggcggc agcatccatc cggcagacgg
     2881 cgtctggacg gggctcgaga ggaccatcaa cgaggcgctg ggcaaggtga gcctggagtc
     2941 cctcgtcaac caggccagag agatcgtgct caactaccag atctgagccg cctgccgccc
     3001 cggctcggag gcatctgggt ggaccgaagg ctgcacaaca aggagatgcg catggcagac
     3061 cggcttgcgg ctcggcagaa gtgggagttg cttctgtccg gcggggatcc agggccgatg
     3121 gtgagccccc tgtgcgatga ctggtccctg gacgttccgt actcctggcc ttaccccgag
     3181 cccgatccgt tccctccggg gcactccaag cacacgctca gtcagcagat ggcaatggcg
     3241 gggctctgcg gatgggagcc gacgtttctc tgcactctgg acatgccacc cagagacgcc
     3301 gaggtgctgc cacgcacggt caccagccag gagggcggcg ccacccggac aacgtcggag
     3361 atccgcactc cctacggtac gctgacttcc atcaccgagg cacagcgtac ctctcgaacc
     3421 ctgaagccct ggatcgagac cgaggcggac ttccatcgga tgagctgggt ggccaggaag
     3481 gtcgcggact acgacgagga tgccgctatc gagcaaggcc gggtcctttg cgccgcggtt
     3541 ggcgatcggg gggtcctggg cacctggttc ggtcctccca tcggctacaa cctgaaccgg
     3601 gacgccatgt tctatcacct ggcggactgg cccgaggctt tcgaggagct gcatcaggcc
     3661 acccgcgctc ttgcgtttcg gcagatcgag acgctgcgca aggccggctt cgactatctc
     3721 ttctactgtg tggatgcgac ggagtggatt tcaccagact tcttccgccg ctggatcctg
     3781 gaggacacgc gacgcctctt tgcgaggtgg cacgagctgg gcggcttcgt gctctggcat
     3841 agctgcggaa aggtcgcgac gttcctggcc caggggttct acaacgacct cgcgcccgat
     3901 gtgctggaaa cgctatccga ggccccgctg ggggacctgc cgagcctgcg gtgggccagg
     3961 gatcggctgg accggaagat catcaccaag gggaacatcg gcctggatat cctgctgggg
     4021 gggaccgagg aagaggtgcg tcgcgaggtg agccgtgtgc gcggcgagac cgccggctac
     4081 cggcacgtcg tcgggctctc ggatgacatc ctcaagggta cgccgcttcg caacgcgcgc
     4141 gccctggtgg aggaatcgag ggcgtcgatc cgctaggcca tctccgcgcc tcccatggcg
     4201 aggtgatcgc gtccaggacg atcatccccg ctggcccggc ggaggtgacc cggccggcag
     4261 gctcggtcgg atgctgttct ccgacccttt tgatcctcga acgcggcgct gacgggaccc
     4321 caagtctgag cgccggtttt tcttgccttt cgcaccggat cggtagcatc ttaggtggca
     4381 atcgatgggt gggttttaca ttgtgaccaa ggagaatgct atccatgaag atgcgaccgg
     4441 ccacctctat cgtggcacta tccttgatcg tgctcctggc ggcgttcggc cttggcggat
     4501 gcggtctggc ccagcagctt ggcttgatag cgccaaacgc tccaaccggg cttgcggctt
     4561 cgtgggtcac gggatcgtcc accgatatcg aggtctcctg gaacagctcc gtgggcgcca
     4621 cgagctacac cctcagtcgc tcctcgaacg gttccagcgg gccattcaag agcgtttaca
     4681 ccggcgcttc cgtcaatttc accgacaccg gtctcagcgc cgcggtctcc tactggtacg
     4741 aggtggtggc aaccagcggc gcgggaagca gcgccaactc ggatatcgtc gaagtctcca
     4801 gccagacgtc ggccgggtct ccggtgatag cgctggcggc agccagcctg gtcttcaccg
     4861 ctacggcggg aggagccgac cccgcggctc agacggtttc cgtcaccaac acgggagccg
     4921 gaacgctgag cggattgagc gccaccgtga gctctagcgg gaacggctgg ctcaccgcgt
     4981 cgttgagcgc caccacggcc cccgcgaccc tgacggttac gccgagcacg gggtctctat
     5041 ccgcgggcac gtacaccgcg acggtgagcg tcgcatcgag cgtgtcgggc gtgacctcga
     5101 agagcgttgg cgtcaccctg acggtctccg gacctccggc aataggactg tcggctgcga
     5161 cgctgacctt caccgcgatg ccgggaggag cggaccccag cgagcagacc gtttcggtca
     5221 ccaacacagg agggggaagc ctgaccgggt tgagcgtcat cgtgagcccc agtggcaacg
     5281 gctggctgac cgcgtcgctg agctcagcgt cggccccggc aacgctgacc gtgacgccga
     5341 gcacgggatc cctggccgcg ggcacctaca cggcaacggt gagcatcgcg tcgagcgtgc
     5401 cgggggtggt tacacagagt gtaggcgtca ccttcacggt ttcgtccgac gtcgggtctg
     5461 acgtgattac tgtccaatag agggtggagt agagatcagg cggtacgatg agagagatcg
     5521 accatcattc caataccagg accatcagga gggatcagga attgcgtaag aatctgtcac
     5581 tcgttttgtt tgctctgctt gttgccctgc tcgtcggctg caccatgccg gagagcccgc
     5641 ttccagcggc gactgccact ggcggcgggt ctgcaaacgg cggtgtcgcc gcagccagca
     5701 gcggctccgc aacgagcagt ggcgctgcca acaccgcggc gggagcaacc gccggcacgt
     5761 tcaagctcac cgtacctcac cttgcgccat gggtcgtgaa ggccaccgcc aaggcatcga
     5821 gcagccgtgc tttcgtggag gccgactccg gcgatttcac gattgtcgac cacaacaacg
     5881 acaatgccac ggtgggttcg ggccctctga acggttccac caccgtcttg gccggtacaa
     5941 ccgcatacac gacgtcgggc gtgctgccgg caggctccta cggcgtgacg gtcaacatct
     6001 acaacgcgaa cgacggcgaa ggcgccgtgg tctccgggga ggctgatttc accgttatcg
     6061 ccggccggac tatccccgtg acggtgacct gcttcccggt gaattccacg acggtggaaa
     6121 tgccggctca gaacaccgag tcggttgccc tcagcccggg cggctacgag agttggtttt
     6181 gggtgccttc cggatacgct acacaaactg gtttcgaagt cacggcgagc aactacggtg
     6241 aatcgttcgg tacggtatac tactttgtct tctatgcgga cggaacgtat accggatcgt
     6301 tcagcacggc aaatcctgac aacccgtcca ccttcgtcga cgtcaatccg tctgaaggcg
     6361 aagcc
//
LOCUS       DASRZS010000611         8516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1507896, whole genome shotgun sequence.
ACCESSION   DASRZS010000611 DASRZS010000000
VERSION     DASRZS010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8516
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507896"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /locus_tag="VFH83_14900"
     CDS             complement(<1..1640)
                     /locus_tag="VFH83_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487714.1"
                     /translation="MRKASVFFVALLLAGAAMVWAGGTKESTAGTSGQAAGASGGVMT
                     PEGKYPQTVEISIPKSASASPNWPKGDSISNNSLTRSILDRLNVQVKIAWEVDQSEYV
                     NKLALNLAANALPDAFTLDSRNNYLFYKQLTDNDMLAELTQAYQLCAGPYMKDTFGSF
                     KNRNLDAYKVNGKMYAIADGAYGYQHELLWVRQDWLTKYNLQAPKTLDDIKNVIKTLV
                     ANKAGGDQTVALLADPLEPVSTIDNSRYNLGPIFESFHAFPKQWIKGSDGKIIWGSTA
                     PEMKQALQVAADWYKEGIIDKQFPTRTANGALDSLVKAGQTGVFFAPWWGPYTWFPDF
                     PKNFPDGLLKAYNAPLDTAGNFNVMWPGPSNAMIVVNKKYAHPEVVVKMLNIEFDAWR
                     GLDKDLAAAVKPEMDAGTDWTYFFPTGGVNLEYADAVPDSGGLVKNWIDTGKMVGKYG
                     HAETAFNQQLAIDAKHYLDTKSLADSGWIGYVGRYQASNILDNPEVKITYPAFSFVTE
                     SMGDLKANLDKLENQTMLAIITGQQPVSSFDAFVTQWKA"
     gene            complement(1866..4295)
                     /locus_tag="VFH83_14905"
     CDS             complement(1866..4295)
                     /locus_tag="VFH83_14905"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET6487715.1"
                     /translation="MRRVKPRPGLEITESIRLQPGIYDFGGADGLRVLADDIEVDGRG
                     VTLRSTAPAPRLPDGDLLYSPGDLGASRETHELLARKILLPAAGPLQLRYRFWGASAD
                     PLTVTVSRDRKTWRSLGPAAATALEGGWSLQRYELRGQAEGEIALKLSVPPESVSRSA
                     PLFYDSFEILRESTSIWRGDARDNWQSWYNTGFSILRRAEKRFYGGVGLRAEGRRGVR
                     LHGLSIHGFLSALVLRGCRGWTVENNDFSDNYDDPDYGWGEGVEQNGAVYLEEVHDSV
                     IRRNRGLRVWNGITLRRCTGNRIERNVFSHCSNTCLKLAQSSANLIRDNVFSWGLRIY
                     PGEVHARDSVSLLVESGSNDNRFLYNDFTHGGDGIFVRVLNHWCSRGNLFQGNDCSHA
                     NNNAVESWSPGNRYVANRANRSSYGFWLGGSDETALVGNQVMHNGKGPRNAPEPFGNA
                     GVSVVHGSSTGFLMVGNRIQDNSGPGVALAWRETKPARLWLIANNSIRWNRNDPRGYR
                     GNGIFAELCRDVAILGNSLRGNEGRALFLGKGCSNVTQAAGRVEVIEGLEIVTDMAAP
                     VVGGEVKLSIRGPGGAQPQGMDDFTWDLGDGEGLSTAEPHAAHRFYRPGRWRVNVTAR
                     GGRRAGIATRCLCVLPRGQQMPQAADPARWRLSSDSTLKPSSAALELDRSQSVLGSGS
                     LKATIRRGTLNTLTLDLGRGLDLRGVSGVSFFYRYACELFFISGKRTRIIGLRLVGEG
                     GSTAEYIPDGNAEELASEDRYEWVYLTAPKTALKGIETMTRAVALQVVFGPEAPADTL
                     FWLDAITVMEG"
     gene            complement(4292..6409)
                     /locus_tag="VFH83_14910"
     CDS             complement(4292..6409)
                     /locus_tag="VFH83_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487716.1"
                     /translation="MGASEAVTLSSDQLRLEVSIADDRVVVRLVHGHGPTGLLLADGP
                     YWYRASRREKEGLVVSDRLAEPILRRDGESVTITGRLAGLELSQVYALRTGRPFLEER
                     ITLRNAGGQPVRLEALDLGPVLRITDPYGSIPAAMAADRLVAVPFRHRPPDPDGVDQD
                     WALADLLRLFGREQRSTISPLAWPQHGWVPSSRRGSEGWAWERGVYALGVYKLNQEVM
                     EYSVVSTEPDADGVWLRFGGAGLFSGEPSTLGEIAPGKTVQLGITRYQVVPNDRREAW
                     YGFRGFLDEHGCGYPKAFDPPVHWNELYDNAEWNLATPGTPKLKGSTRALTYTRQALE
                     LEAAKAAAYGCQSLYLDPGWDTAFGSLQWGEAWLGNRREFIEGIRQRYGLGLSLHCPL
                     ATWMSMDGRGIAAWPREAYQKSKDGKVLEGAVCLGSRQYLDEAERRLSEHCADGVVYL
                     MFDGNWWNGGCWNPDHGHPVPYTMQDHARAQFELARRVHRKHPSVIIEQHDPISGGVL
                     HRHSPVYFGYGPGSFDVSWGFELMWQPMEDILSGRARSLYWYNLGCNVPVYLHIDLRD
                     DNANLLVLWWYASTCRHLGIGGTHAIPAVARAHQEAMARYRRLDRFYKRGVFYGAHEE
                     AHIHVLPEENGFVVNLFNLSPHERVIECSVRFDRLGLDPDRWYWMPQAGRVDKPSQSF
                     ILAKRLPPWSAHVAEVRSIEAAS"
     gene            6680..8516
                     /locus_tag="VFH83_14915"
                     /pseudo
     CDS             6680..8516
                     /locus_tag="VFH83_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003740.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
BASE COUNT         1386 a         2867 c         2826 g         1437 t
ORIGIN      
        1 gccttccact gggtgacgaa ggcgtcaaag gaggacaccg gctgctggcc ggtgatgatg
       61 gccagcatcg tctggttctc cagcttgtcc aggtttgcct tcaggtctcc catggactcc
      121 gtcacgaagg agaacgccgg ataggtgatc ttgacttccg ggttgtccag gatgttggag
      181 gcctggtacc ggcccacgta gccgatccag ccgctgtccg ccagagactt ggtgtccagg
      241 tagtgcttgg cgtcgatggc cagctgctgg ttgaaggccg tctcggcgtg accgtacttg
      301 cccaccatct tgccggtgtc gatccagttc ttcacgagcc cgccgctatc ggggaccgcg
      361 tcggcgtact ccaggttcac gccgccggtc gggaagaagt acgtccagtc ggtgccggcg
      421 tccatctcgg gcttcacggc cgcggccaga tccttgtcca gaccgcgcca cgcgtcaaac
      481 tcgatgttga gcatcttcac gacgacttcg gggtgcgcgt acttcttgtt gactacgatc
      541 atggcgttgg acgggcccgg ccacatgacg ttgaagttgc cggcggtgtc caggggggcg
      601 ttgtaggcct tgagcaggcc gtcggggaag ttcttcggga agtcagggaa ccaggtgtac
      661 ggaccccacc acggagcgaa gaacacgccg gtctggccgg ccttgaccag ggagtccaag
      721 gcgccgttgg cggtgcgggt ggggaactgc ttgtcgatga tgccttcctt gtaccagtca
      781 gcggctacct gcagggcctg cttcatttcg ggcgcggtgg agccccagat gatcttgcca
      841 tcggagcctt tgatccactg cttggggaac gcgtggaagg actcgaagat cggacccagg
      901 ttgtagcggg agttgtcaat ggtgctgacc ggctccaacg ggtccgccag cagggcgacc
      961 gtctggtcgc cgccggcctt gttggcgacc aacgtcttga tgacgttctt gatgtcgtcc
     1021 agcgtcttgg gggcctgcag gttgtacttg gtcagccagt cctggcggac ccacaggagc
     1081 tcgtgctgat agccgtaggc gccgtcggcg atggcgtaca tcttgccgtt gaccttgtac
     1141 gcgtccaggt tccgattctt gaagctgccg aacgtgtcct tcatgtaggg gccggcgcat
     1201 agctgatagg cctgtgtcag ctcggccagc atgtcgttgt ccgtcaactg cttgtagaac
     1261 aggtagttgt tgcgggagtc cagggtgaat gcatccggca gcgcgttggc ggccaggttc
     1321 agcgccagct tgttcacgta ctcgctctgg tcaacctccc aggcgatctt cacctggacg
     1381 ttcagccggt ccaggatgga gcgggtcagc gagttgttgc tgatcgaatc gcccttgggc
     1441 cagttcgggg aagccgatgc ggacttgggg atcgagatct cgacggtctg cggatacttg
     1501 ccttccggag tcataacccc tccggaagcg ccggcggcct ggccgctggt gccggccgtg
     1561 gactccttgg tcccgccggc ccacaccatg gccgcgcccg ccaggagcag ggccacgaag
     1621 aaaacggaag cctttcgcat gggaacctcc ttgagcgtgg aacgaacctg tcgacggcca
     1681 gagacaggcc agggcctgtc gtcgcgcgga gaggcgatcc acggtcgctg tttccctctg
     1741 gccttttagc agcgggtact ataggaccgc ccggggtacc tgtcaagttt gcttgaggct
     1801 gccggggcgc cgggcggcgg ggccggggcg ccgggcggcg gggcggcgaa accgctcccg
     1861 gcggctcacc cttccatcac cgtgatcgcg tccagccaga agagggtgtc agcgggtgcc
     1921 tcggggccga agaccacctg gagcgcgacg gcgcgcgtca tcgtctcgat ccccttcaga
     1981 gccgtcttcg gcgccgtgag ataaacccac tcgtagcggt cctcgctggc gagctcctcc
     2041 gcgttcccgt ccgggatgta ctccgcggtc gagccgccct cgcctaccag gcgcagtccg
     2101 atgatccggg tgcgtttgcc ggagatgaag aacagctcgc aggcatagcg gtagaagaac
     2161 gagacgccgc tgaccccgcg aaggtcgagg ccgcgcccga ggtccagcgt cagggtattg
     2221 agcgtccccc ggcggatcgt cgccttcagg ctcccggaac caaggacgga ctgagaccga
     2281 tcgagctcca gagcggcgga ggagggtttg agggtggagt ccgaggatag ccgccagcgc
     2341 gcgggatctg ccgcttgcgg catctgctgt ccccgcggca ggacgcagag gcaccgggtg
     2401 gcgatgcccg cccgcctgcc gccccgagcg gtgacgttga cgcgccagcg gccgggccgg
     2461 tagaaccgat gggccgcgtg gggctccgcg gtcgacagcc cctcgccatc gccaaggtcc
     2521 caggtgaagt catccatgcc ttgaggctgc gcgcctcctg ggccgcgaat ggacagcttc
     2581 acctcgcccc cgacgacggg ggcagccatg tccgtcacga tctccagccc ctcgatgacc
     2641 tcgacccgcc cggccgcctg ggtaacgttc gagcaccctt tgcccaggaa gagcgcgcgc
     2701 ccttcgttcc cccgaaggga gttccccagg atggccacgt cgcggcacag ctcggcgaag
     2761 atcccgttgc cgcgatagcc gcgaggatcg ttgcggttcc atcggatgga gttgttggcg
     2821 atcagccaga gccgggccgg cttggtctcg cgccaggcca acgccactcc gggcccgctg
     2881 ttgtcctgga tccggttgcc caccatgaga aagccggtcg aggatccgtg gaccacggac
     2941 acccccgcgt tcccgaacgg ctccggcgcg ttgcgcgggc ccttcccgtt gtgcatgacc
     3001 tggttgccca cgagcgccgt ctcgtccgac ccgcccaacc agaacccgta gctggagcga
     3061 ttggcccgat tggcgacgta ccggtttccc ggggaccagg actccaccgc attgttgttg
     3121 gcgtgagagc agtcgtttcc ctggaacagg tttccgcggc tgcaccagtg gttgagcacc
     3181 cggacaaaga tgccgtcccc accgtgcgtg aagtcgttgt agaggaagcg gttgtcgttc
     3241 gagcctgact ccaccaggag gctcacggag tcccgggcat ggacctcgcc ggggtagatc
     3301 cgcagccccc aggagaagac gttgtcccgg atgaggttcg cggagctctg cgccagcttg
     3361 aggcaggtgt tggagcagtg cgagaagacg ttgcgctcga tgcggtttcc cgtacaccgt
     3421 cggagggtga tcccgttcca gacccgaagc ccccggttgc gccggatgac cgagtcgtgg
     3481 acctcctcca ggtacaccgc gccgttctgc tccacgcctt caccccagcc gtagtccgga
     3541 tcgtcgtagt tgtcggagaa gtcgttgttc tcgaccgtcc atccccgaca gcctcggagc
     3601 accagcgccg acaggaaccc atggatggag agcccgtgaa gccgcacgcc gcggcggcct
     3661 tccgcccgca gccccacgcc gccatagaat cgcttttcgg ctcgacggag gatcgagaag
     3721 cccgtgttgt accagctttg ccagttgtct cgcgcatccc ccctccagat cgaggtcgac
     3781 tcccgaagga tctcgaagga gtcgtagaag aggggcgccg agcgtgacac cgactcggga
     3841 gggaccgaaa gcttcaaggc gatctcccct tccgcctggc cgcgaagctc gtaccgctga
     3901 aggctccagc cgccctccag agccgtggcg gcggccggcc cgaggctccg ccacgtcttg
     3961 cggtctcggg aaaccgtcac ggtgagcggg tccgcgctgg cgccccagaa ccgataccgt
     4021 agctgaagcg gcccggcagc gggcaggagg atcttccgcg ccaggagctc gtgggtttcc
     4081 cgtgaggcgc cgaggtctcc cggggagtac agcaggtctc cgtccggcag ccggggagcc
     4141 ggagccgtag accgcaaggt cacgccgcgg ccatcgacct cgatatcgtc ggcgagcacc
     4201 ctcagaccgt ccgcgcctcc aaaatcgtag atacccggct gcaggcggat gctctcggtg
     4261 atctcgaggc ccggccgcgg tttgacacgc ctcacgatgc cgcctcgatg gagcggacct
     4321 ccgccacgtg cgcggaccac ggcggcagcc gcttcgccag gatgaagctc tgggacggct
     4381 tgtccacgcg gccggcctgg ggcatccagt accagcggtc cgggtccagc cctagccgat
     4441 cgaaccggac gctgcactcg atgacgcgct cgtgcgggga aagattgaag aggttcacca
     4501 caaagccgtt ctcctcgggc agcacgtgga tgtgcgcctc ctcgtgcgcg ccgtagaaca
     4561 ccccgcgctt gtagaaccgg tccaggcgcc tgtagcgcgc catggcctcc tggtgggccc
     4621 gggccacggc cgggatcgcg tgggtgcccc cgattcccag gtgccggcag gtggaggcgt
     4681 accaccacag gaccaggagg ttggcgttgt cgtcccgcag atcgatgtgc aggtaaaccg
     4741 ggacgttgca gccgaggttg taccaataga ggctccgggc gcgccccgag aggatgtcct
     4801 ccatgggctg ccacatcagc tcgaagcccc agctcacgtc gaagctgccg ggcccatacc
     4861 cgaagtagac cggggagtgg cgatgcagca ccccgccgga gatcgggtca tgctgctcga
     4921 tgatcaccga ggggtgcttc cgatggaccc gccgagccag ctcgaactgg gctcgcgcgt
     4981 ggtcctgcat ggtatagggg accggatgcc cgtggtcggg gttccaacac ccaccgttcc
     5041 accagttgcc gtcgaacatg aggtagacga caccgtccgc gcagtgctcg ctgagccgtc
     5101 gctccgcctc atccaggtac tgtcgagagc ccaggcacac cgcgccttcc agcaccttcc
     5161 catccttcga cttctggtac gcctcccgag gccacgcggc gatgccccgg ccatccatcg
     5221 acatccaggt ggccagggga cagtggagcg agagacccag cccgtaccgc tggcggatcc
     5281 cctcgatgaa ctcccgccga ttccccagcc acgcctctcc ccactgcaga gagccgaacg
     5341 cggtgtccca tcccgggtcc aggtacaagg actgacagcc gtaggcagcg gccttggccg
     5401 cctcgagctc cagggcctgg cgcgtgtagg tcagcgcgcg ggttgagccc ttgagcttcg
     5461 gggtcccagg ggtcgccagg ttccactccg cgttgtcgta aagctcgttc cagtgcaccg
     5521 ggggatcgaa tgccttcggg tagccgcagc cgtgctcgtc cagaaagccg cggaagccgt
     5581 accaggcttc ccgccggtca ttcggaacca cttgatatcg cgtgatcccg agctggacgg
     5641 tctttcctgg ggcgatctcg ccaagggtcg acggctctcc ggagaacagg ccggccccgc
     5701 cgaagcgcag ccagacgcca tcggcgtccg gctcggtgga gacgacgctg tattccatga
     5761 cctcctggtt gagcttgtag acgcccaggg cgtacacgcc ccgctcccat gcccagccct
     5821 cggagccgcg tcgagaggac gggacccagc cgtgctgagg ccaggccagg ggagagatgg
     5881 tggaccgctg ctctcggccg aagaggcgca gcaggtcagc aagcgcccag tcctgatcaa
     5941 caccatccgg atctggtggg cgatgtcgga acgggaccgc caccaggcgg tccgcggcca
     6001 tcgcggcggg gatactgccg taagggtcgg tgatccgcag caccggcccc aggtccaacg
     6061 cctccaggcg tacgggctgg ccgccggcgt tccgaagcgt gatccgctct tccagaaacg
     6121 gacgaccggt tcggagggcg tagacctgcg acagttccag gccggccagc cgaccggtga
     6181 tggtcaccga ttccccatcc cggcggagga tcggctctgc cagccgatcc gagaccacga
     6241 ggccctcctt ctcccgacgg ctggcccggt accagtaggg tccgtccgca aggaggaggc
     6301 cggtgggacc gtggccgtgc accagacgca caaccacccg gtcgtcggcg atggagactt
     6361 cgagtcgaag ctggtcgctg gacagggtaa ccgcttcgga tgcgcccatg gggacctccc
     6421 gtctgctcga tgccacttcc taccgcgccc tgcgcttttg gtcgaggggg cggttcggag
     6481 ccggacggcg gcctcggtcg gtcgcggcgc ttgaacaagc gcacagcggt gagtaccttc
     6541 gcttctgcac atgagagctc cagccaacgg tcgcaagcct ggcgcggctg ccgcgcgctc
     6601 cgggtccggg gctcccccgt ccgcccgccc cgggtccggg gctcacccgg cagcggcatc
     6661 ttccgcggat cccgatcaca tcgtcatcga gggcgcttcg cagaacaacc tgcgcaacct
     6721 ctcgctgaag ctgccgcttc ggaagctcat cgccatcacc ggcgtgagtg gctcggggaa
     6781 gtcctccctg gccttcgaca cgctgtacgc cgagggtcag cggcggtaag tggagacgtt
     6841 ctcctcctac acccgtcagt tcctggaccg gatggaccga ccgcgcgtgg atcggatcga
     6901 ggggatccct cccgccatcg ccatcgatca gaccaatccc gtgcggacct cgcggtccac
     6961 cgtgggcacg atgaccgagc tgtccgatca cctcaagctg ctcttcgccc gggcagccag
     7021 gctctactgc gggaactgcg gccgcgaggt gcgacgcgac acggcggaga gcatcgtgga
     7081 ccagctcccc caggggcggc ggctcatcct gaccttcccc atcacagtgc ccgcgagctt
     7141 cacggaggcc gaggtcgagg agctcctggc caagcagggc tacacccgaa tcctgcgccg
     7201 ggacggagcc cgtctggacg tgatccagga ccgcatcacc tccagtccgg ccaaccgggc
     7261 ccggatcgtg gaagacctgg aggccgcgct gaagtacggg cagggacggc tcggcgccca
     7321 cctcgtggac gagaagggcg agccgaccga gaccctccag ttctcttcgg ggctgcactg
     7381 cgcgttctgc gacatccgct acgacgatcc atcccccaac ctcttctcgt tcaattcgcc
     7441 gctgggcgcg tgcccgagct gtcgcggttt cggcaggacc atcggcatcg actgggacct
     7501 cgtgataccg gatgacacca agagccttgc cggcggcgcc gttcggccgt ggcagaccga
     7561 aagcttccgg gaggtccagg gggacctcct gaagttcgct cgacgccgag gcatccccac
     7621 ggatgctccc tggcgatctc tgactccgca gcagcggtcc tgggtcatcg acggcgaggg
     7681 ggattgggac gatggggtct ggtacggcgc caggcggttc ttcggctggc tcgaatcgaa
     7741 gagttaccgg atgcacatcc gggtcctcct ctcgaagtat cggtcctata cgctgtgccc
     7801 cacctgcaac ggagcgagac tccgccccga gacgctcctc tggaagctcg gcgaggagaa
     7861 gctctccctc catgacgtgg tcctgcttcc catcgaccgg tgcatggcct tcttcgccgg
     7921 cctgaagctc ccggcgcctc tggacgaggc aaccgagctc cttctgtccg agatccagac
     7981 ccgtctgcgc tacctggtgg acgtcggcct ggggtacctc accctggatc gccagtcccg
     8041 gaccctgagc ggtggagaag tgcagaggat caacctcacc accgccctgg gcacctccct
     8101 ggtcaacgca ctcttcgtgc tggatgagcc cagcatcggg ctccatccgc gggacatcgg
     8161 ccggctgatc gccgtcctgc gacgtctgcg ggatgcggga aacacgatcg tcgtggtgga
     8221 gcacgatccc gacgtaatcc gagccgcaga cctggtcctg gacatgggtc ctgggcctgg
     8281 agagcagggc gggcagatcg tattcttcgg caagctcagc gagcttctca agagcaagag
     8341 gtcgctcacc gcgcagtacc tgacgggcaa gaagcgggtg gaggcccggg gcgtcgacgg
     8401 tgtagcggca ggcggcggct cagccggcag cgccggcacc gggcccgacc agcaccccgc
     8461 tcggccgcgg cccgccccca agctccgcat cgtgggggcc gcggaacaca acctca
//
LOCUS       DASRZS010000612         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2662477, whole genome shotgun sequence.
ACCESSION   DASRZS010000612 DASRZS010000000
VERSION     DASRZS010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2662477"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VFH83_14920"
     CDS             complement(<1..974)
                     /locus_tag="VFH83_14920"
                     /inference="COORDINATES: protein motif:HMM:NF017659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HET6487717.1"
                     /translation="MGYVAVQGGIAAIESSQNLNRYLRLRGDAPPLSVLQILEQMRPL
                     VDRVMGEGGLYAPFHAALALKQASGDPLEASFLLRAYRSTLRRLVDSLPIRTDAMQIR
                     RRISSAFKTIPGGQVLGSTSDYEQRILDFSLAEETPETQEETRDAFWSSLPSITDSLR
                     PESFRKVTDILREQGLVPAAADGEPSAAVDITQHAAELPLPRSGRLQMLARAETGGVL
                     ALAYSSMRGYGSVHPTVAELRVGRIRVQIPHPWRQGQAVTVGWVVVTEVEIVASVQRS
                     EGEEKPAFSLGYGACFGHNEVKAISIAVLDRAMMSTSPHAPAEDQEFVL"
     gene            complement(974..1594)
                     /gene="phnH"
                     /locus_tag="VFH83_14925"
     CDS             complement(974..1594)
                     /gene="phnH"
                     /locus_tag="VFH83_14925"
                     /inference="COORDINATES: protein motif:HMM:NF017645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HET6487718.1"
                     /translation="MTRRETVFDEVLDSQATFRALLDCMARPGTVAQLPLADYSAAPP
                     AFCPPALSILKTLVDHRVSFSVAGASGRPDWIEYLRLNLSAPFQGVDAADYVLFDGAM
                     YHDDFSRLKPGSLEFPESSATAVLAVAALFGARSAGDDPGSCYLSLSGPGVRDRETLS
                     MRGFDPRYIEARARANRLFPIGVDLLLVDANRRVAGLPRTSHVEVG"
     gene            complement(1605..2045)
                     /locus_tag="VFH83_14930"
     CDS             complement(1605..2045)
                     /locus_tag="VFH83_14930"
                     /inference="COORDINATES: protein motif:HMM:NF018464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HET6487719.1"
                     /translation="MDYSEIVAEASVEAAREMADWVVRLLPVKVLRPPSPGMVMVRHV
                     DPLENTLFLLGEAYVLECEVEVDGLLGYGCTLGSGGVGAQSSPNADDERALCAALVDA
                     VVGGQHRLASELLPLLQAEAARIAARREEEARATASTRVSFEVR"
BASE COUNT          430 a          806 c          796 g          377 t
ORIGIN      
        1 aggacgaact cctggtcctc cgccggcgcg tggggggagg tggacatcat ggcccggtcc
       61 agaaccgcaa tcgagatggc tttcacctcg ttgtgtccga agcaggcgcc atacccgagg
      121 ctgaaggcag gcttttcctc cccttcggag cgttggaccg aggccacgat ctccacctcc
      181 gtgacgacca cccatcccac cgtgactgcc tgcccctggc gccacgggtg ggggatctgg
      241 acccggatcc ggcccacgcg gagctccgcc acggtgggat gcacgctccc gtagccgcgc
      301 atgctgctgt aggcaagcgc caggacaccg ccggtctccg cgcgcgccag catctggagc
      361 cgtccgcttc ggggcagagg cagctcggcg gcatgctggg taatgtccac cgcggcggac
      421 ggctctccgt cggcggccgc ggggacaaga ccctgctccc tcaagatgtc ggtgaccttg
      481 cggaagctct cgggcctgag actgtccgtg atcgacggga gagagctcca gaaggcgtcc
      541 cgggtttcct cttgggtctc tggagtctcc tccgcgagag agaagtccag gatccgctgc
      601 tcgtagtccg acgtcgagcc caacacctgt cctccgggaa tcgtcttgaa ggcgctggag
      661 atgcgcctgc ggatctgcat ggcgtcggtg cggatgggaa gggaatcgac caggcgccgc
      721 aaggtggagc gataggcccg caacagaaac gatgcctcca gcggatcccc cgaggcctgt
      781 ttcaaagcaa gcgccgcgtg gaacggggcg tacagcccgc cttcccccat cacccggtca
      841 accagcgggc gcatctgttc caggatctga agcaccgaca gcggcggggc gtctccgcga
      901 agccgcaggt atcggttgag gttttgcgag ctctcgatgg cggcgatccc accctggacg
      961 gcaacgtacc ccatcagccg acctcgacgt ggctggtgcg cggaagcccg gccacgcgac
     1021 ggttggcgtc taccaggaga aggtcgacgc cgatggggaa cagccgattg gccctggccc
     1081 gcgcctcgat gtatcgcggg tcgaagcccc gcatcgacag tgtctcccgg tcccgcacgc
     1141 ccggaccgct cagagacagg tagcaggagc cgggatcatc gccggcggac cgagcgccga
     1201 agagcgccgc aaccgccagg accgcggttg ccgagctctc cggaaactcc agcgagccgg
     1261 gtttcaggcg cgagaagtcg tcgtggtaca tcgcgccgtc gaagaggacg taatcggccg
     1321 catcgacgcc ctggaacggg gcggagagat tcaaccgaag gtattcgatc cagtccggcc
     1381 gaccggatgc gccggcgacc gagaaggaga cccggtgatc caccagggtc ttgaggatcg
     1441 aaagcgcggg cgggcagaac gcgggaggag ccgcggagta gtccgccagt ggaagctgcg
     1501 ccaccgtccc cggccgtgcc atgcagtcca gcagagcccg aaaggtggcc tggctgtcta
     1561 ggacctcgtc gaagaccgtc tcgcgtctgg tcattccgga cgtcctaccg cacctcgaaa
     1621 ctgacccggg tggaggcggt ggcccgcgcc tcctcctccc gccgggccgc gatccgggcg
     1681 gcctccgcct gcaggagcgg aagaagctcg gaggccaggc gatgctgacc gccgacgacg
     1741 gcatccacca gcgcggcgca cagggcgcgc tcatcgtccg cgttcgggga gctttgagcc
     1801 ccgacgccgc cggaccccag ggtgcagccg tagcccagga ggccgtccac ttccacctcg
     1861 cactccagga cgtaggcctc tcccaggaga aagagcgtgt tctccagggg gtccacgtgt
     1921 cggaccatca ccatgccggg agaaggaggt ctcagcacct tcacgggtag gagccggacc
     1981 acccagtccg ccatctctcg ggccgcctcg acgcttgcct ccgccacgat ctccgagtaa
     2041 tccatcgacc tcaaacagac cactccccgg ttagcaccgg atgaggagcc cgttaagagt
     2101 ctgttgattt cccgacctcc cgccgtcctc ggaacggcgc ggggaccgag gattaacaga
     2161 ggattaacca aatcctcaca aacgattaac ctttgttcgg gatgatcctt gcggcgctgc
     2221 gagtacggcg ggttgtcgca tcgcggctcc cctgaaaacg gattcgtgat ctggatgtag
     2281 gttccatggg tcgacgagtc gccgccatag acgttgggac gaacgcaatc cgcttctacg
     2341 cggtcgaagt ggacggagac tctgtctctc gcgtgcttac cgaggagcgc tttgcggtcc
     2401 gcctgggac
//
LOCUS       DASRZS010000613         5982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_346359, whole genome shotgun sequence.
ACCESSION   DASRZS010000613 DASRZS010000000
VERSION     DASRZS010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346359"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1446)
                     /locus_tag="VFH83_14935"
     CDS             complement(124..1446)
                     /locus_tag="VFH83_14935"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET6487720.1"
                     /translation="MIRSLLIANRGEIAVRIIRTCREMGIRTTVAYSQADRDSLAVRM
                     ADSHVCVGPPPAKDSYLNVRSLITAAVLSGCDALHPGVGFLAENSGFARDVREAGLVF
                     VGPDPEVIDLLGDKIRAKRSATDMGISVIPGSKGAVNGAKEAVAAANGIGYPMIVKAA
                     AGGGGKGMRIVRSADELSGVLAVASHEAEKAFSDGTVYLERYLENPRHVEIQILADYH
                     GTVVHLGERDCTVQQNHQKLVEESPSMAVSPELRSRMGDDAVRLLKGLGYTGAGTVEF
                     LVKDGKHYFMEVNARVQVEHPVSEMVTGVDIIRQQLLAASGEPLEIRQQTIAVQGYAV
                     ECRINARSAGRVTQFIPPGGFGVRMDTFLYTGCQVPPWYDHLVGKLIVSAASRPEGLR
                     RMERALGELVIEGITTNRDSQRAIISNPVFRKGEYGTGFMAEVLKEGV"
     gene            complement(1443..1895)
                     /gene="accB"
                     /locus_tag="VFH83_14940"
     CDS             complement(1443..1895)
                     /gene="accB"
                     /locus_tag="VFH83_14940"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET6487721.1"
                     /translation="MMDLKELYALMERFEKSGLTELSLKRDGEEVCLKKAGPVQVPVI
                     AQPSVMAPAAAEHRAVSPEAAAKTAPDTEVIRSPLVGTFYRAAAPDAPPFVEKGDKVQ
                     KGKPLCILEAMKLMNEFQAEFDLEIVNILVDNAKMVEFATPLFEVRRV"
     gene            2146..2571
                     /gene="fabZ"
                     /locus_tag="VFH83_14945"
     CDS             2146..2571
                     /gene="fabZ"
                     /locus_tag="VFH83_14945"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET6487722.1"
                     /translation="MTDIKSLIPHREPFLFVDRIERADAKEIVGYRRFPPTEFFFAGH
                     FPEYPVVPGVILVECLAQCGGVGHRMVTETDSGVFFLAAIEKVKFRRQVRPGEEVRMV
                     IQNLRVGRTMLRQAGKSYVGDELAVEAEWLCILGAGVAS"
     gene            complement(2583..4748)
                     /locus_tag="VFH83_14950"
     CDS             complement(2583..4748)
                     /locus_tag="VFH83_14950"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6487723.1"
                     /translation="MTRTCVALLLLALAGSTPVAGADLGSDWTQTRGTGGEIVLSSQG
                     TLPPYSAALLIGPTLWARAVTLDGVSITPGSSSGAPAATGYASVPIPGIPDGGSHRLV
                     VEASDSATVSARPYLALVPADGAAARAVGLNVLVVVLREFSGLASLLLAVLVLLLITR
                     ERTRELLLLVAALPLNALSELSPSLLVSFLGVTVAQRLSVIGLLLTGVLVLYYAVETA
                     KPSPYLTFLPFALVAALAAALALAGSSAVMVEWSALAVRAVFSIAFAVLAAIAASTLA
                     RTKLTKMVTPILLGAALSLSFAASLASDVVWPGRAFLAFLPGLTVAVFQAVLLVSELL
                     RSQSMYRQTSQELIDRIESDWEMIERIREGKELLEKRNAEIMKLAGKLLESAQKQSFT
                     IGRLIESLADAGDGESRVVAKEKDILGYTEQVEGLITSFNLQIQETLQEMEALYQRSI
                     VIRKAVSQIIGIAERTHMLSLNASIEASKAGAAGKGFSVVAQEIRKLAELTRTVSDQV
                     TAVIKDTNRGVEKGVGRIKGLGTGFSEIMASSDETRTMISDNARALEEVSSAHRKIQD
                     GLAGVDTLIRSILEVSHDMRQMTGRLASAFSWFGQTLKLRTDEGSETRRLGSEPSGSA
                     GAPASTDGAEEPASSPLGGWDGALTDGAQPPPLDLGDGGFVLPGGTEAEEAPFAPPGG
                     ESPPDARQPQEGQLQEAELPEDFGELLPVDGSEEEDRRA"
     gene            4813..>5982
                     /locus_tag="VFH83_14955"
     CDS             4813..>5982
                     /locus_tag="VFH83_14955"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET6487724.1"
                     /translation="MASRIDIAEIQKAALRSIPLTFRLTSLPHESHALLDRILEVYLA
                     ELGQEQILEPLSYCLKELIINAQKANTKRVYFEEKGLDLNDRAQYEKGMEEFRRESAE
                     RMDHWISRLKERKLSIAVTFHSTGSHLTIAVRNAVELSQKEQMRIFDRIARARAFGSF
                     VEALSTPLDSSEGAGLGIMILMQFLKRIGLGEEAFSIESQNGETVAAVSIPMNRVHLQ
                     KISVLTTAIVRDVDSLPHFPDAVLRLIRLTEEPSSSVAQIAGEIATDPAVTADLLKLA
                     NSARYVLPQRVNNILQAVRIVGTRGLRNLLYSYGAHEILAEKFTEMETLWARSNRAAY
                     YSYLMARSLKRKEDILDDAYVAGLLSDLGLIIVTYLHPQAMEKMTRFCKEKDIPVR"
BASE COUNT         1043 a         2017 c         1921 g         1001 t
ORIGIN      
        1 ccgggcgctg gcaggatggg caggagttct tctcgtcgat gaagagcctc tcgtcccgct
       61 tcttgtccac ggggtgctcg agcagctgtc ggatccaccc ctggatgccg tctctgcggt
      121 tcactaaacg ccctccttga gcacctcggc catgaagccg gtcccgtatt cgccctttcg
      181 gaacacggga ttcgagatga tggcccgttg ggaatcccgg ttggtcgtga tcccctcgat
      241 gaccagctcc cccagcgcgc gctccatccg gcgaagcccc tccggccggc tcgccgccga
      301 gacgatgagc ttgcccacca ggtggtcgta ccaagggggg acctggcagc cggtatacag
      361 gaacgtgtcc atgcggactc cgaagccgcc ggggggaatg aactgcgtga cccgaccggc
      421 cgagcgcgcg ttgatccggc actctaccgc gtagccttgg accgcaatgg tctgctggcg
      481 gatctccagg ggctcgccgc tggcggcaag gagctgctgt cggatgatgt ccacgccggt
      541 gaccatttcc gagacagggt gctccacctg gacccgcgcg ttgacctcca tgaagtagtg
      601 cttgccgtcc ttcacgagga actccaccgt cccggctccc gtgtacccca ggcctttcag
      661 gaggcggacc gcgtcatcac ccatacggct gcggagctcc gggctcactg ccatggatgg
      721 gctctcctcc acaagcttct ggtggttctg ctgcaccgtg cagtcccgct cccccaggtg
      781 gaccacggtt ccgtggtagt ccgccaggat ctggatctcg acgtgccgcg ggttctccag
      841 gtaccgctcc aggtacacgg tgccgtcgga gaacgccttc tccgcctcgt gagaggccac
      901 ggcaaggacc ccggagagct catccgcgct tcgaacgatc cgcatgccct tgccgccgcc
      961 cccggcagcc gccttcacga tcatggggta cccgatcccg ttggcggcgg ccaccgcctc
     1021 cttggcaccg ttcaccgcgc ccttgcttcc gggaatgacg gagatcccca tgtccgtggc
     1081 ggacctcttg gcgcggatct tatcgcccag gaggtcgatc acctccgggt ccggacctac
     1141 gaagacaagc ccggcctctc ggacgtcccg ggcaaagccc gagttctccg ccaggaaccc
     1201 gacgccggga tgcagggcat cgcatccgct caggaccgcc gccgtgatga ggctgcggac
     1261 gttgaggtag ctgtccttgg ccggtggggg tcccacgcag acgtggctgt ccgccatgcg
     1321 gacggcaagg gagtcccggt ccgcctggga gtaggccacg gtggtccgta tccccatctc
     1381 gcgacacgtg cggatgatcc gaacggcgat ctccccgcgg ttggcgatga ggaggcttcg
     1441 gatcataccc ggcgcacctc gaacagaggg gtggcaaact ccaccatctt ggcgttgtcc
     1501 accaggatgt tcacgatctc caggtcgaac tcggcctgga actcgttcat gagcttcatg
     1561 gcctccagga tgcacaacgg cttgcccttc tggaccttgt cccccttctc gacgaaggga
     1621 ggcgcatccg gcgcggcggc ccggtagaag gtgccgacga ggggcgatcg gatcacctcc
     1681 gtgtccggcg cggtcttggc ggcggcctcc ggggagacgg cgcggtgctc cgcggccgcc
     1741 ggggccatca cgctgggctg agcgatcacg gggacctgaa cgggacctgc cttcttcagg
     1801 cagacttctt cgccatctct cttcaggctg agctcggtga ggccgctctt ctcgaagcgc
     1861 tccataaggg catagagctc cttaaggtcc atcatgcatc cccctctgtc tccgccgtga
     1921 gcttctcatc cgaatcgtgg ccatctgact cgcttcaagc atgacaagtt tggtcggaaa
     1981 ttagcaagag cccccccgag tgtcaattcg cgtcgggacg gccgcgcggg aggcgtacgg
     2041 cggaaggccg ctcgggcagc gcgcgattcg ggcgcaaccc acggcagctc cggccctgaa
     2101 actcgccact tctcccacga tagggatcgt gttaccctgc gccgcatgac cgacatcaag
     2161 tccctgatcc cccaccgaga gccgtttctg ttcgtggatc gcatcgagcg ggcggacgcc
     2221 aaggagatcg ttggataccg gcggtttccg ccgacggagt tttttttcgc ggggcatttt
     2281 ccggagtatc ccgtggtacc cggcgtcatc cttgtggaat gtctggccca atgcgggggg
     2341 gtcggccacc gcatggtgac ggagaccgac tcgggggtgt tctttctcgc cgccatagag
     2401 aaggtgaagt tcagacgcca ggtgaggccc ggcgaggagg tgcggatggt gatccagaac
     2461 ctccgcgtcg gacggacgat gctgaggcag gcggggaagt catacgtggg ggatgagctt
     2521 gccgtcgagg ccgagtggct ctgcatcctc ggggcagggg tcgcctcgta gctccgccgc
     2581 cgtcaggcgc gccggtcttc ctcttccgac ccatccacgg gcagaagctc gccgaagtct
     2641 tccgggagct ctgcctcctg gagctgacct tcctggggct gacgggcgtc cggagggctt
     2701 tcgccgccgg ggggagcgaa cggagcctct tcggcctccg ttccgcccgg aaggacgaac
     2761 ccaccatctc ccaggtccag gggcggaggc tgggctccgt cggtcagagc gccgtcccag
     2821 ccgcccagcg gcgatgaggc cggctcctcg gcgccgtcgg tggacgccgg tgcccccgcg
     2881 gagccggaag gctccgagcc cagcctccgc gtctcggacc cctcgtcggt gcgaagcttg
     2941 agggtctggc cgaaccagga gaacgcggac gccagccgcc cggtcatctg ccgcatgtcg
     3001 tgcgatacct cgaggatgga ccggatcagc gtatcaacac cggcaaggcc gtcctggatc
     3061 tttcggtggg cgctgctcac ctcttccagg gcgcgcgcgt tgtcggagat catggtacgc
     3121 gtctcgtcgc tgctcgccat gatctccgag aatcccgtgc ccagtccctt gatccggccc
     3181 acgcccttct ccacgccccg gttggtgtcc ttgatgacgg ccgtcacctg gtccgacacg
     3241 gtgcgggtca gctcggcaag cttgcggatc tcctgcgcaa ccacggaaaa ccccttgccg
     3301 gcggcccccg ccttggacgc ctcgatcgag gcgttcaggg aaagcatgtg ggtccgctcg
     3361 gcgattccaa tgatctggct gaccgctttt cggatcacga tggaccgttg atagagcgcc
     3421 tccatctcct ggagcgtctc ctggatctgg aggttgaagc tggtaatgag cccttccacc
     3481 tgttccgtgt agccgaggat gtccttctcc ttggccacca cccgggactc tccatccccc
     3541 gcgtccgcca gggactcgat cagccttccg atggtgaagg actgcttctg agcgctctcc
     3601 agcagcttcc ccgccagctt catgatctcc gcgttgcgct tctccagcag ctccttgccc
     3661 tcccggatgc gctcgatcat ctcccagtcg ctctcgatcc ggtcgatcag ctcctgactg
     3721 gtctgacggt acatcgactg ggaccgaagg agctccgaca ccaggaggac cgcctggaac
     3781 accgcaaccg tcaggcccgg caggaacgca aggaacgcgc ggcccggcca taccacgtcg
     3841 gacgccaggg aagccgcgaa ggatagggaa agggccgcgc ccaggaggat gggcgtgacc
     3901 atcttcgtca gcttggtccg cgccagggtc gaagccgcga tcgcggcgag caccgcgaag
     3961 gcgatggaga agaccgcccg tacggccagt gccgaccact cgaccatgac cgcgctggat
     4021 ccggccagcg ccagcgcagc ggccagagcc gcaaccagcg cgaacggcag gaacgtcaga
     4081 tagggggagg gcttggcggt ctccaccgcg tagtacaaca cgaggacacc ggtgagcagg
     4141 aggccgatca ccgaaaggcg ctgggccacc gtcacgccga ggaaggacac gaggagggac
     4201 ggggacagct cggaaagggc gttcagcgga agagccgcga ccaggagcag gagctcccgg
     4261 gtacgttccc gggtgatgag cagaagcacc aggacagcga gcagcagcga ggccaggccg
     4321 ctgaactccc gcagcaccac gacgaggacg ttgaggccga ccgcgcgcgc cgcggcgccg
     4381 tcggccggaa ccagggccag gtacggtcgc gccgagaccg tcgcggagtc cgaggcctcg
     4441 accacgagcc gatgcgagcc gccatccgga atgccgggaa tcggcaccga agcgtatccc
     4501 gtcgccgccg gcgcgccaga ggaggacccg ggcgtgatgc tgacgccgtc cagggtcacc
     4561 gcgcgcgccc agagcgtcgg cccgatcagc agagcggcgg aatacggagg cagcgtgccc
     4621 tgactggaga ggacgatctc tcccccggtc ccgcgcgtct gcgtccagtc ggaccccagg
     4681 tccgcgcccg ccacgggtgt cgagccggca agcgccagga gcagcaacgc cacgcaggtt
     4741 cgagtcatct ttccgaagta tagagcgaaa ggtagaagaa taaaaggttc tgctgctata
     4801 ctacggttca gaatggcatc gagaatcgac atcgcggaaa tccagaaggc cgcacttcga
     4861 tcgataccac tcaccttccg ccttacgagc ctcccccacg agtcccacgc cctgctggac
     4921 cggatcctcg aagtctacct ggccgagctg ggacaggagc agatcctgga gcctctctcg
     4981 tactgcctga aggagctgat catcaacgct cagaaggcca acaccaagcg ggtgtacttc
     5041 gaagagaagg gcctggatct caacgaccgg gcgcaatacg agaaggggat ggaggagttc
     5101 cgccgcgaat cggcggaacg gatggaccac tggatctccc ggctcaagga gcgcaagctc
     5161 agcatcgccg tcacctttca cagcaccggc tcccatctga ccattgcggt gcgcaacgcg
     5221 gtcgagcttt cccagaaaga gcagatgcgg atcttcgatc ggatcgcccg ggcgcgggcc
     5281 ttcgggtcct tcgtggaggc gctctccacg ccgctggact ccagcgaagg cgccggactg
     5341 gggatcatga tcctcatgca gttcctgaaa cggatcggtc tgggagagga ggccttcagc
     5401 atcgagagcc agaacgggga gacggtggcc gccgtgtcca tcccgatgaa ccgcgtgcat
     5461 ttgcagaaga tcagcgtgct cacgacggcc atcgtgcggg acgtggactc cctgccccac
     5521 ttcccggatg ccgtgctgcg cctcatccga ctgaccgaag agccgagctc cagcgtcgcg
     5581 cagatcgctg gagagatcgc cacggaccct gccgtgaccg cggacctgct caagctcgcg
     5641 aactccgccc gctacgtcct cccccagcgc gtgaacaaca tcctccaggc cgtgcggatc
     5701 gtcggcaccc gggggctccg caacctcctg tactcctatg gggcccacga gatcctggcg
     5761 gagaagttca cggaaatgga gacgctctgg gcacgctcca atcgcgccgc ctactactcg
     5821 tacctcatgg cccgaagcct caaacgcaag gaggacatcc tcgatgacgc gtacgtcgcc
     5881 gggctgctga gcgatctggg gctcatcatc gtcacgtacc tccatcccca ggcaatggag
     5941 aagatgacgc ggttctgcaa ggagaaggac atcccggtcc gg
//
LOCUS       DASRZS010000614         3601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1889948, whole genome shotgun sequence.
ACCESSION   DASRZS010000614 DASRZS010000000
VERSION     DASRZS010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889948"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1348)
                     /locus_tag="VFH83_14960"
     CDS             complement(50..1348)
                     /locus_tag="VFH83_14960"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HET6487725.1"
                     /translation="MSRNALVAQSGGPSPVINASLQGVIEGCRAYPALIGKRYAAWHG
                     IEGVLQEELIDLSRQPQSEIEALGTTPAAGAIGTCRYKLTDGQAEDFERIVEVIRAHD
                     IGFFFYIGGNDSMDTAAKVSRLARERGVELVVAGVPKTIDNDIGDAEFKIIDHTPGYG
                     SAARYWAYLTQNVNEESRGMCVSEPVAVLQAMGRKSGFITAAARLGDPRREMPLQIYM
                     AESKHTLESLHENVNRELRRSGRCIVVVSEGFDVGSVGERHDGFGHIEYGASKTNAAQ
                     EVVNYLNEKGLAVRGQATGQVPGVIQRSVSLFASRVDIEEARAVARHAVEVAVTDGTG
                     WMSTTLRRPGPRYQAYYDKVPLETVANSSRFMPPSWITPDGLDVTDDFVRYASPLIGE
                     GNPDIRLEGGLQRFARFDVRFVDRVCRPYVPVRFRETARA"
     gene            complement(1341..1751)
                     /locus_tag="VFH83_14965"
     CDS             complement(1341..1751)
                     /locus_tag="VFH83_14965"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET6487726.1"
                     /translation="MIERLSEEMVRAMLETLPVEITVIDANDEVAGWNKHETRLFHRP
                     LTSMGLNFRDCHPAESLPRVERIVSEMRSGQRDRARFWIDLPVPPDTEKHKILIEFYA
                     LRDPTGKYLGCMECTQDTQEIRGLQGQRRLLDDE"
     gene            complement(1778..>3601)
                     /gene="pepF"
                     /locus_tag="VFH83_14970"
     CDS             complement(1778..>3601)
                     /gene="pepF"
                     /locus_tag="VFH83_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET6487727.1"
                     /translation="RKDGTMPASVPPRSKIDPKHTWNAESVFRSPKAWEEALASVLSD
                     LSGLKQFAGHLAEGPDTLLRCLKATEDLQRRAYHLFVYANFASSVDTADQQAAGMNGR
                     AQGMMGQVFAAASFLRPELLAVGRAALDEWMRANPDLAVYRHSFDDLFRKQKHVRSGE
                     VEELLGMLADPFSGPSTTASVMANADFVFPPAVDSQGKKTPVTQSSYYTVILGHRDRA
                     VRRSAFEGYMDRHLAFKNTLASNLTTSIKANVLAARARRHESSLHAALFEHSIPPEVF
                     HGLISTFRRKLPVWHRYFDIRRRALKQRDVCYYDMWAPLGRPGKKIPFERAVQIIADA
                     LSPMGKDYAETIRTGCLEQRWVDIYPNQGKQHGAFSSGAPGTHPFVMMSYTGELESVS
                     TLAHELGHSMHSHLTWTHQPIVYADYSMFVAEVASNFHQAMVRAHLLATETSKDLQIG
                     VIEEAMSNFFRYFFQMPTLARFELETHQRVEAGQSLTADSLIELMADLFSEAFGPGVK
                     IDRPRVGMVWSTFAHLFIDYYVFQYATGISGAHALSGRILRKEPGAVEDYLGFLRAGS
                     SDYPLEVLRRAGVDLTTPKPVEETFSIMEGYIDRLEKLLFG"
BASE COUNT          599 a         1188 c         1171 g          643 t
ORIGIN      
        1 cgcggtgcgg gcctcaggcg cagtgcgagc ctgaaccgcg ggacgagcct tatgcccgtg
       61 ccgtttcccg aaaccgaacg ggtacataag gcctgcaaac gcggtccacg aaccgcacgt
      121 cgaaccgcgc gaaccgctga aggccaccct ccaggcggat atccggattg ccctccccga
      181 tgaggggtga ggcgtatcgc acgaagtcat ccgtcacgtc caggccgtcc ggcgtgatcc
      241 aggacggcgg catgaatcgc gacgagttgg ccaccgtctc cagcggcacc ttgtcgtaat
      301 aggcctggta gcgcggcccc ggcctgcgaa gggtggtgga catccagccg gtgccgtcgg
      361 tgaccgctac ctccaccgcg tgtcgggcaa ccgcgcgggc ctcctcgatg tcgacccggg
      421 aggcgaagag cgagaccgat ctctggatca ccccagggac ttggccggtt gcctgacccc
      481 ggacggccag ccccttctcg ttcaggtagt tcacgacctc ctgcgccgcg ttggtcttgc
      541 tggccccgta ctcgatgtgg ccgaaaccgt catgacgctc gcccacgctt cccacgtcga
      601 acccctcgct caccaccacg atgcatcgcc cggaccggcg gagctcgcgg ttgacgttct
      661 catggaggct ctccagggta tgcttgctct ccgccatgta gatctggagc ggcatctcgc
      721 ggcgggggtc tccgagccgg gcggcggccg tgatgaagcc ggacttgcgt cccatggcct
      781 ggagaacggc caccggctcc gagacgcaca tcccgcggct ctcctcgttc acgttctggg
      841 tcaggtaggc ccagtagcgc gccgcgctcc cgtatcctgg ggtgtggtcg atgatcttga
      901 actcggcgtc gccgatgtcg ttgtcgatcg tcttgggcac gcccgccacc acgagctcca
      961 ctccgcgctc ccgggcaagc ctggagacct tggccgcggt atccatagag tcgttgccac
     1021 caatatagaa gaaaaaaccg atgtcgtggg cccgaatgac ctcaacgatc cgctcgaagt
     1081 cttccgcctg gccgtcggtg agcttgtacc ggcacgtccc gatggcgccg gcggccggcg
     1141 tggtacccag ggcctcgatc tcgctttgag gttgtcggga aagatcgatg agctcttcct
     1201 ggagcactcc ctcgatgccg tgccaggccg cgtaccgttt accgatgagg gccgggtagg
     1261 cccggcaacc ctcgatcacc ccctggaggg acgcgttgat cacgggcgac ggcccgccgg
     1321 actgggcaac cagcgcgttt ctactcatcg tcgaggagcc tcctctggcc ctggagtccg
     1381 cggatctcct gcgtgtcctg ggtgcactcc atgcagccaa ggtacttccc cgtggggtcg
     1441 cgcagcgcgt agaactcgat caggatcttg tgcttttccg tgtcgggcgg caccggcagg
     1501 tcgatccaga accgggcccg gtcccgttgg cccgagcgca tctccgacac gatgcgctcc
     1561 acccggggca ggctctccgc gggatgacag tctcggaagt tgagccccat ggaggtgagc
     1621 ggccggtgga acagccgggt ctcgtgcttg ttccatcccg ccacctcgtc attcgcgtcg
     1681 atcacggtta tctcgacggg caacgtctcc agcatcgccc ggaccatctc ttcactcaag
     1741 cgctcgatca aatggacctc ctgagtcccg acaggctcta tccgaacaga agcttctcca
     1801 gccgatcgat atacccctcc atgatggaga aggtctcttc cacgggcttc ggggtggtca
     1861 ggtccacccc ggcccggcgc agcacttcca gagggtagtc cgaagagccg gcccggagaa
     1921 accccaggta gtcttcgacc gcgccgggct cctttctcag gatgcggcct gagagggcgt
     1981 gagcgcccga gatccccgtg gcgtactgga acacgtagta gtcgatgaag agatgggcaa
     2041 aggtggacca caccatgccc acccgcgggc ggtcgatctt cacgccgggc ccgaatgcct
     2101 ccgagaagag atccgccatc agctcgatca gggagtccgc ggtcagcgac tgtcccgcct
     2161 ccacgcgctg gtgcgtctcc agctcgaacc gggccagcgt gggcatctgg aagaagtagc
     2221 ggaagaagtt ggacatggcc tcctcgatca cgccgatctg gaggtccttg ctcgtctcgg
     2281 tggccaggag atgcgcccgc accatggcct ggtggaagtt ggaggccacc tcggccacga
     2341 acatcgagta gtccgcgtag acgatgggct gatgcgtcca ggtgaggtgg gagtgcatgg
     2401 agtggccaag ctcgtgcgcc agcgtgctta cgctctccag ctcgcccgta tagctcatca
     2461 tcacgaacgg atgggtgccg ggcgcgccgg aggagaacgc cccgtgctgc tttccctggt
     2521 tggggtagat gtcgacccag cgctgctcca ggcagcccgt gcggatggtc tccgcgtagt
     2581 ccttccccat cggagagagc gcatcagcga tgatctggac ggcacgctcg aaggggatct
     2641 tctttcccgg gcggcccagc ggcgcccaca tgtcgtagta gcagacatcg cgctgcttga
     2701 gggcccggcg gcggatgtcg aaataccggt gccacaccgg gagcttccgg cggaaggtcg
     2761 agatcaggcc gtggaacacc tcgggcggaa tgctgtgctc gaagagcgcc gcgtgcaggc
     2821 tggactcatg gcggcgggct cgggccgcca gcacgttcgc cttgatggag gtggtgaggt
     2881 tgctggccag cgtgttcttg aaagccagat gccggtccat gtagccctcg aaggcgctgc
     2941 gtcggacggc ccgatcccga tggcccagaa tgaccgtgta gtagctgctc tgggtcaccg
     3001 gcgtcttctt cccctgggag tccacggccg gaggaaacac gaagtccgcg ttggccatca
     3061 cgctcgccgt agtggatggt ccgctgaacg gatccgccag cattcccagg agctcctcca
     3121 cctccccgga acgcacgtgc ttctgcttgc ggaaaagatc atcaaagctg tgccgataca
     3181 ccgcgaggtc cgggttggcg cgcatccact cgtccagggc cgcgcgtccg actgccagga
     3241 gctcgggccg cagaaacgac gccgcggcaa acacctggcc catcatgccc tgggcccggc
     3301 cgttcatccc ggcggcttgc tggtccgcgg tgtccacgct ggacgcgaag ttcgcataga
     3361 caaagaggtg gtaggcgcgc ctctgcaggt cctcggtggc cttgaggcac cgtaggaggg
     3421 tgtccggtcc ctcggccagg tggcccgcga actgcttgag tcccgagaga tcggagagga
     3481 ccgaggccag cgcttcttcc cacgccttcg gggaacggaa gacggactcg gcgttccagg
     3541 tgtgcttcgg atcgatcttg ctccggggag gtaccgatgc gggcatagtg ccgtcctttc
     3601 t
//
LOCUS       DASRZS010000615         4863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2665415, whole genome shotgun sequence.
ACCESSION   DASRZS010000615 DASRZS010000000
VERSION     DASRZS010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2665415"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VFH83_14975"
     CDS             complement(<1..541)
                     /locus_tag="VFH83_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487728.1"
                     /translation="MAAKGRPGEPEGLKEQLRDFHGEGVFPWQFSNESQRKRWLEWHY
                     DEEKYWGGFSREPFDPATYRLPEWAIGPFTKHAGNPVVAPDPTGWDCGHFGGGVHNGS
                     VLKRDGRLFLIYRGEFPIPEEARFAAHRTAGFDYLCDVGVAVSDDGVRFRRVAGPLLR
                     RPQDWRFSFEDVNCVQHDGR"
     gene            complement(632..2101)
                     /locus_tag="VFH83_14980"
     CDS             complement(632..2101)
                     /locus_tag="VFH83_14980"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6487729.1"
                     /translation="MKRFVVIALAVLVTVGAFGQATVRYVMPGDASPDNDTVIAAVNA
                     RLGPDAGLKLETVYVPWDAWENKINLMLASGDEFDLVHVMQDWIPYTSYFSKGGLADI
                     TDSIAKYGANLVKNIPAAMWPGAKVKGRTYVIPTFWAELATEGDFTIRTDFLKQNGLS
                     LPRTPAEMLTAMETVAKNWKGSDKPYLPLAPSVNNADPIGMHSSVLHRAYASFPFTVK
                     EKLFYVSQSGQARSWVETPEFKQDAAWFRSAYVKGLISPDVLTIKQDQVVNQINSGNW
                     LVSWGTIGSYKDLQKNWPALQDADILTAMFNPEKPDVRPWGIKNCNAVPSTTTNPDLG
                     VKFMNWLYASQSNYDLFLYGVEGKHYTTLRAHQKDSVMDPTGRPLYIQADWMMGNLNF
                     IRLDSVIPAATIKDIYTIDSKAINSPAADLFFDSSNVRAELANVMSQFSAVMLPVYMG
                     VVDWDKNYPAALDKMKKAGLDRLVAEYQKQLAAFLAAHK"
     gene            complement(2168..3013)
                     /locus_tag="VFH83_14985"
     CDS             complement(2168..3013)
                     /locus_tag="VFH83_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6487730.1"
                     /translation="MYFLVAVFALICAFPLVLSVIVSFTDEDAIRRNGYAFAPEKWSV
                     YSYRLIFHSGSSVLRSYGISTFVTVVGTVLAVAITAMAAYTLANKRVQYRRWLALFFY
                     VTMVFTAGIVPWYFICRAMGLRNNILSLIVPSLLFNPFNLFLVKNYMDGIPDSLRESA
                     TIDGASDIRTAFSIYFPLAIPVLATVTLFYGLAYWNDWWNAIMLVDDKKLYPLQFLLL
                     QLRSQIAMIRDLQLGAAGASTITPPAESMKMATAVVTIGPIVLLYPYLQRYFIKGLVV
                     GSIKG"
     gene            complement(3049..3972)
                     /locus_tag="VFH83_14990"
     CDS             complement(3049..3972)
                     /locus_tag="VFH83_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009589944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6487731.1"
                     /translation="MKTRRGLLGDILRNPASYLLALPAIAYTLAFSYLSYPYLLMAFQ
                     RFNYRRNIWNAEYIGLTNFQFFFRANYAYTITWNTLKLNTLFIVFTTVVALLLALMLN
                     ELASKRFVKVSQSTMVFPTYLSWVVVSYMLFGIFSMEYGLLNRVLVRLGGEAINWYIR
                     AEAWPAILTGMRVWKGAGLNAVIFLAAIAAIDDTLYEAASIDGASRWQRAWTITLPLI
                     APTVAILTLMAIGRIMYGDFGMIYALIGDNGPLYPTTDVIDTYVYRALRKVGDPAEAT
                     AIGLFQSAVGFVMVIGANTLTRKLFPEGALY"
     gene            4134..>4863
                     /locus_tag="VFH83_14995"
     CDS             4134..>4863
                     /locus_tag="VFH83_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487732.1"
                     /translation="MTTPFGLLRYRVARRLMVSYFVLAVLTIVALSSILFALFARSAA
                     AQIAAGSRELLQRTAYAADVLDSQVQVIGSYLINDLDVVLYLYSKEDDKVLAYNAMRV
                     LDRIISEYTFIRSIGLYNRTTGGYLNNAGMASRVPFPDSISPEIRLTPRVISAADSPD
                     RRSHPVLTGMLYPYAFSGAPETGAIELNIDEGYLQSLIAGIAGSAERSVTMVMDSSGM
                     VLSHPDPGRFMRDLSKEGYASRILG"
BASE COUNT          899 a         1498 c         1577 g          889 t
ORIGIN      
        1 agcgcccatc gtgctggacg cagttcacat cctcgaaact gaacctccag tcctggggtc
       61 ggcgcagcag gggcccggcc acgcgccgaa agcgtacacc gtcgtcgctc acggccaccc
      121 cgacgtcaca gagataatcg aagccggcgg tccgatgcgc cgcgaaccgg gcctcctccg
      181 ggatggggaa ctcaccccga tagatgagga agagccggcc gtcccgcttc aggacggacc
      241 cgttgtgcac tcccccgccg aagtggccgc aatcccaccc cgttggatcg ggcgcaacca
      301 ccgggttgcc ggcatgcttc gtaaacggtc cgatggccca ctcgggcaga cggtacgtgg
      361 ccggatcaaa gggttcccgg gagaagccgc cccagtactt ctcctcgtcg tagtgccatt
      421 ccagccagcg cttcctctga ctctcgttgg agaactgcca cggaaagacg ccttctccgt
      481 ggaagtcccg aagctgctcc ttgagcccct cgggttctcc gggccggccc ttcgctgcca
      541 tgcgctcatc ccccctggcc gtccgggatc gatcccggca gaccgttgga gcccgggggt
      601 cccgggctcc gtcgatcggt gacgcgtgaa tctacttgtg ggcggccagg aaggccgcaa
      661 gctgcttctg gtactccgca acgagcctgt cgagacccgc cttcttcatc ttgtccagcg
      721 ccgcggggta gttcttgtcc cagtccacca cgcccatgta caccgggagc atgacggccg
      781 agaactggct catgacgttg gccagctcgg cccggacgtt gctggaatcg aaaaacaggt
      841 cggcggccgg actgttgatg gccttcgaat cgatggtgta gatgtccttg atggtggccg
      901 cagggatgac ggagtccagc cgtatgaagt tcaagttccc catcatccag tcggcctgga
      961 tgtagagcgg ccgacccgtc gggtccatga cggagtcctt ctggtgcgcg cgcagcgtgg
     1021 tgtagtgctt cccctcgacc ccgtacagga agaggtcata gttcgactgg ctcgcgtaca
     1081 accagttcat gaacttcacc cccaggtcgg ggttggtcgt ggtggagggc accgcgttgc
     1141 agttcttgat gccccagggc cgcacatcgg gcttctccgg gttgaacatg gcggtgagga
     1201 tgtccgcgtc ctggagcgcc ggccagttct tctggaggtc cttgtagctg ccgatggtcc
     1261 cccagcttac cagccagttg ccggagttga tctggttgac cacctggtcc tgcttgatcg
     1321 taaggacgtc cgggctgatg aggcccttga cgtacgcgga gcggaaccag gcggcatcct
     1381 gcttgaactc gggggtctcg acccaggacc tggcctggcc cgactggctg acgtagaaca
     1441 gcttctcctt caccgtgaag gggaagctcg cgtaggcgcg gtgcaggacg ctggaatgca
     1501 tgccgatggg atcggcgttg ttgacgctgg gcgcgagggg caagtagggc ttgtcgctgc
     1561 ccttccagtt cttcgcgacc gtctccatgg cggtaagcat ctcggcggga gtccggggca
     1621 gggagaggcc gttctgcttc aggaagtcgg tccggatggt gaagtctccc tcggtcgcca
     1681 gctccgccca gaacgtcggg atcacgtaag tgcggccctt gaccttggcc ccaggccaca
     1741 tcgccgcggg tatgttcttc acgaggttgg ccccgtactt cgctatcgaa tcggtgatgt
     1801 cggccagacc tcccttggag aagtagctgg tataggggat ccagtcctgc atcacgtgga
     1861 ccaggtcgaa ctcatccccg ctggcgagca tcaggttgat cttgttttcc caggcatccc
     1921 aggggacgta cacggtttcc agcttgaggc ccgcatcggg cccgagcctg gcgttgacgg
     1981 cggcgatgac cgtgtcgttg tccggcgagg cgtcgccggg catgacgtag cgcaccgtgg
     2041 cctgaccgaa ggctccgacg gtcaccagga ccgccagggc aatcacgacg aaacgcttca
     2101 tacttccctc cttcgccaaa ggcgtatggt tcagtcgggc ggcgacccgc gggacgcacg
     2161 cgcgacctta tcccttgatc gagcccacca ccaggccctt gatgaagtag cgctggaggt
     2221 agggatagag cagcacgatg ggaccgatgg tcaccacggc cgttgccatc ttcatggact
     2281 ccgccggggg cgtgatcgtc gaggcgccgg cggcgcctag ctgcaagtcc cggatcatcg
     2341 cgatctggga ccgtagctgc aggagaagga actgcagcgg gtagagcttc ttgtcgtcca
     2401 ccagcatgat cgcgttccac cagtcgttcc agtacgccag cccgtagaac agggtgacgg
     2461 tggcaagcac cgggatggcc agggggaagt aaatgctgaa cgcggtccgg atgtcgctgg
     2521 cgccatcgat ggtggcgctc tcgcgcaggc tgtccgggat cccgtccatg tagttcttga
     2581 ccagaaagag gttgaacggg ttgaacagga ggctgggcac gatcagggaa aggatgttgt
     2641 tgcgcaaccc catggcccgg cagatgaagt accacgggac gatgccggcc gtgaacacca
     2701 tggtgacgta gaagaacagg gcgagccagc ggcggtactg cacgcgcttg ttggccaggg
     2761 tgtaggccgc catggcggtg atcgccacgg cgaggacggt gcccacgacg gtaacgaagg
     2821 tcgagatccc gtagcttcgc aggacgctcg atccggaatg gaagatcagc cgataggagt
     2881 acaccgacca cttctcgggg gcgaacgcgt agccgttgcg gcggatggcg tcctcgtccg
     2941 taaaggagac gatcaccgag aggaccaacg ggaacgcgca gatcagcgcg aagaccgcga
     3001 ccaggaagta catcagcgca ctcgaggcac gacccccctg ccgcatggtc agtacaacgc
     3061 tccttccggg aagagctttc gggtaagcgt attggccccg atcaccatga cgaagcccac
     3121 ggccgactgg aacaacccga tggccgttgc ctcggcggga tctcccactt tgcgcagcgc
     3181 ccggtagacg taggtgtcga tgacatcggt ggttggatag aggggcccgt tgtcgccgat
     3241 cagggcatag atcatgccga agtccccgta catgatccgg ccgatggcca tgagcgtgag
     3301 gatggccacc gtgggagcga taagcgggag ggtgatggtc catgcccgct gccaccggga
     3361 tgccccgtcg atggaagccg cctcgtagag ggtgtcgtcg atcgcggcga tcgccgcgag
     3421 gaagatcacg gcgttgagcc ccgccccttt ccacaccctc atcccggtca ggatcgcggg
     3481 ccatgcctcc gcgcggatgt accagttgat cgcctcgccg cccaggcgca cgagaacccg
     3541 gttgaggagc ccgtactcca tggagaagat cccgaacagc atgtagctca ccaccaccca
     3601 cgagaggtag gtgggaaaca ccatcgtgga ttgggagacc ttgacgaacc gcttgctggc
     3661 aagctcgttc agcatgagcg ccaggagcag cgcaacgacg gtggtgaaca cgatgaagag
     3721 cgtgttgagc ttcaacgtgt tccaggtgat cgtgtatgcg tagttcgctc gaaagaagaa
     3781 ctggaagttg gtgagcccga tgtactcagc gttccagatg ttcctgcggt agttgaagcg
     3841 ctggaacgcc atgagcaggt agggatagga aagataggag aaggccaggg tgtaggcgat
     3901 tgccggcagc gcgagaaggt acgacgccgg atttcgcagt atgtctccca ggagcccccg
     3961 gcgtgtcttc acaaacgccg atgataggtc cgccggccgg gcgctgtcca tttcgaagtc
     4021 cggcggtggc gtttgtccag gccgaaacgg gctttcgaaa tcgggagaaa gcttccacga
     4081 agcggtcaaa cgctccggtt gcagttgcgg tggcggaaac gtagaatcgg cccatgacga
     4141 caccattcgg tctgctgcgc tatcgagtcg ctcgcaggct catggtgtcg tacttcgtcc
     4201 ttgcggtcct caccatcgtc gcgctgagct cgatcctctt tgctcttttc gccaggagcg
     4261 ccgccgcgca gatcgctgcc ggttcgcgcg agctgctgca gcggaccgcc tacgccgccg
     4321 acgtcctgga cagccaggta caggtcatcg gcagctacct catcaacgac cttgacgtcg
     4381 tcctttacct gtactccaag gaggacgaca aggtcctcgc ctacaacgcg atgcgggtcc
     4441 tggaccgaat catctccgag tacacgttca tccgcagcat cggcctgtac aaccgcacta
     4501 cgggcggcta cctgaacaac gcgggaatgg cctcgcgggt accgttcccg gactcgatca
     4561 gccccgagat ccgcctgacg ccgcgggtga tctccgcggc ggacagccct gaccgaaggt
     4621 cgcacccggt gcttaccggg atgctgtacc cctatgcctt ctcgggggct cccgagaccg
     4681 gcgccattga gctgaacatc gatgagggct acctgcagtc cctcatcgcg ggcatcgccg
     4741 ggtccgcgga gaggtcggtg acgatggtga tggactcgtc cggtatggtc ctgtcccacc
     4801 cggatcccgg ccggttcatg cgcgatctct cgaaggaagg gtacgcatcc cggatcctgg
     4861 gaa
//
LOCUS       DASRZS010000616         3577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_347906, whole genome shotgun sequence.
ACCESSION   DASRZS010000616 DASRZS010000000
VERSION     DASRZS010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347906"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1207)
                     /locus_tag="VFH83_15000"
     CDS             complement(170..1207)
                     /locus_tag="VFH83_15000"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HET6487733.1"
                     /translation="MDANAAGNGKLLEVVDLKKYFPIQRGFFKVTVGHIKAVDGVSFS
                     IRQGETLGLVGESGCGKTTLGRCIVRLYDATAGSILFYENGEAVTVMDLKGRELRAFR
                     RNMQIVFQDPYASLNPRMTVQDIVGEPLVIHGLASGKELQDRVEEMLAFVGLQREHMR
                     RYPHSFSGGQRQRIGIARALVLRPKLVVADEPVSALDVSIQAQTLNLLKRMQADLQLT
                     YLFVSHDLGVVRYVSDRIAVMYVGKLVELSGKDNVLATPRHPYTESLLSAVPRVDLHY
                     RTERIILPGEAPDPSNLPPGCVFHTRCRYAKDECTQVEPKLREISTGHFVACHRADQF
                     ELRGVSHRVQR"
     gene            complement(1218..2228)
                     /locus_tag="VFH83_15005"
     CDS             complement(1218..2228)
                     /locus_tag="VFH83_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487734.1"
                     /translation="MVSAGELLLEVRDLQAHFHLLEGTVRAVDGVSFDVSCGSTVGII
                     GESGSGKSVMAQAILRIIPSPPGKIMGGKVLLHDLKPGIEPLDLTTLPPDGDVLRGIR
                     GNSISMIFQEPMASFGPLTTIGSQISEAILTHTPGCGKKEAMDRTVELLARVGIPKPS
                     QRKDSYPYQFSGGMLQRAMIAMALSCSPRLLIADEPTTAVDVTIQAQLMELLARLQEE
                     RHMAILLITHNLAVVSELAQHIVVMYLGRIVERSTVQAIFESPLHPYTRALWRSIPKV
                     EGELTTLKSIQGTIPSPYETLTGCPFFNRCEERMVGKCDRVEPPTVEPQPGHWVSCLR
                     YV"
     gene            complement(2240..3379)
                     /locus_tag="VFH83_15010"
     CDS             complement(2240..3379)
                     /locus_tag="VFH83_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487735.1"
                     /translation="MAMTILPGGRRERKLQSQAEQLYYASQWQLIWRKFRMHRLATIS
                     IVGLVILYLAAIFCEFVSPYGARTRFDNYVSAPPQRIRILRPGHGLQEPFMYGFTQKR
                     DPVTLRMGTALNKDEVHRVRFFVHGEPYRMWNIIPGDIHLFGADGPVPLFLFGSDTLG
                     RDIFSRIIYGARISLSIGLVGVLLSFLLGVIIGSISGYFGGVMDTIIQRLIEFLISIP
                     TLPLWMVLSGLLPRDWSTVKTYFAITLLLSVVGWCGLARVVRGKLLALREEDFVNAAR
                     AAGATQGRIILRHLLPGMTSYLIVNITISIPGMILGETSLSFLGLGLQPPAVSWGVLL
                     QDAQNIVTVAHHPWQLIPVIFVVVTVLLFNFLGDGLRDAADPYTR"
     gene            complement(3395..>3577)
                     /locus_tag="VFH83_15015"
     CDS             complement(3395..>3577)
                     /locus_tag="VFH83_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017590224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487736.1"
                     /translation="VLVSIVLNMPTSGPVLLRSIMYQDVYLAGGFFLILSTLTIVGTL
                     LSDILLAWLDPRIRYQ"
BASE COUNT          648 a         1121 c         1231 g          577 t
ORIGIN      
        1 atcgtggcgg aggagtgcaa cgggtacctg agctacatcc cgaccgccga cgagtacccc
       61 gagggcggtt acggttcagc cgccgccatc ctggcgccgg aggcagaggc agtcctgctg
      121 gaagccacgg agaccctggc ccgccgctgc ctctcctgag cgtgcaggcc taccgctgga
      181 cccggtggga gacgcctcgc agctcgaact ggtccgcgcg gtggcaggca acgaagtggc
      241 cggtggagat ctcccgcagc ttgggctcta cctgggtgca ctcgtccttg gcgtaccggc
      301 agcgcgtgtg gaacacgcac ccgggcggca ggttggatgg atccggcgcc tccccgggga
      361 ggatgatccg ctcggtccgg tagtggaggt ccacgcgcgg cacggcggag agaagcgact
      421 cggtgtaggg gtgacgcggc gtagccagca cgttgtcctt gccggagagc tcgaccagct
      481 tgcccacgta catcaccgcg atccgatcgc tcacgtagcg gaccacgccc aggtcgtggg
      541 acacgaagag gtaggtgagc tgcaggtccg cctgcatgcg cttcaggagg ttcagcgtct
      601 gggcctggat cgaaacgtcc agcgcggaaa ccggctcgtc cgccaccacc agcttgggac
      661 gaagcaccag ggcgcgcgcg atgccgatcc gctggcgttg gccgccggag aaggagtgcg
      721 ggtagcggcg catgtgctcg cgctgcagac cgacgaaggc cagcatctcc tcgacgcgat
      781 cctgcagctc cttgccggac gcgagcccat ggatgaccag cggctcccct acgatgtcct
      841 gcaccgtcat gcgggggttg agggaggcgt aaggatcctg gaacacgatc tgcatgttcc
      901 gacggaacgc ccgaagctcc ctgcccttga ggtccatgac cgtgacggct tccccgttct
      961 cgtagaagag gatcgagccc gccgtggcgt cgtagagacg cacgatgcat cgccccaggg
     1021 tggtcttgcc gcacccggac tcccctacca gcccgagggt ttccccctgt cggatggaga
     1081 aagagacccc gtccaccgct ttgatgtggc caacggtgac cttgaagaag ccccgctgga
     1141 tggggaagta cttcttgagg tccaccactt ccaggagctt gccgttgcct gccgcgttcg
     1201 catccatgtc gttcccccta gacgtagcgc aggcagctga cccagtgccc tggctggggc
     1261 tccacggtcg gcggctccac ccggtcgcac ttcccaacca tgcgctcctc acagcggttg
     1321 aagaacgggc atcccgtaag ggtctcgtag ggcgagggga tggttccctg gatggacttc
     1381 agcgtagtga gctccccttc cactttgggg atggagcgcc agagggcgcg ggtatacggg
     1441 tgaagcgggc tctcgaagat ggcctggacg gtggaccgct ccacgatccg gcccaggtac
     1501 atgaccacga tgtgctgggc cagctcggag accacggcca ggttgtgggt gatcaacagg
     1561 atcgccatgt ggcgctcttc ctggagacgc gccagcagct ccatgagctg cgcctggatg
     1621 gtgacgtcca cggcggtggt gggctcatcg gcgatgagga gcctcggcga acaggagagc
     1681 gccatggcga tcatcgcccg ctggagcatt ccaccggaga actggtatgg gtaggagtcc
     1741 ttgcgctggc tgggcttggg gatgccgaca cgggccagaa gctccacggt ccgatccatc
     1801 gcctctttct tgccgcagcc gggggtgtgg gtgaggatgg cttcggagat ctggctgccg
     1861 atggtagtca ggggtccgaa ggaagccatg ggctcctgga agatcatgga gatcgagttg
     1921 ccgcgaatcc cgcgaagcac gtcaccgtcc ggcgggagcg tggtgaggtc caggggctcg
     1981 atgccgggct tcaggtcgtg cagcaggacc ttgcccccca tgatcttccc cgggggcgac
     2041 gggatgatcc gcagaatggc ctgggccatc acggacttgc ccgagccgct ctccccgatg
     2101 atcccgacgg tggatccgca agacacatcg aagctgaccc cgtccaccgc gcggacggtt
     2161 ccctccagca ggtggaagtg cgcttggagg tctcttactt ccaggaggag ctcgccggcg
     2221 ctgaccatgg cccctcccgc taccgcgtgt agggatcggc cgcgtcccgc aagccgtccc
     2281 ccaggaagtt gaacagcagc accgtcacca ccacgaagat cacggggatg agctgccagg
     2341 ggtgatgcgc caccgtgacg atgttctggg cgtcctgcag gagcacgccc cagctcaccg
     2401 ccgggggctg gagccccagc cccaggaagc tgaggctggt ctctcccagg atcatcccgg
     2461 ggatgctgat cgtgatgttg acgatcaggt agctcgtcat cccgggcaga aggtggcgca
     2521 ggatgatgcg tccctgcgtg gcgcccgcgg cccgcgccgc gttcacgaag tcctcctccc
     2581 gcagggccag gagcttcccg cgcaccaccc gggccagacc gcaccagccc acgacggaga
     2641 gaagaagggt gatggcgaag taggtcttca ccgtggacca gtctcggggg agcaatccgg
     2701 agagcaccat ccacaacgga agcgtgggaa tcgagatcag gaactcgatg aggcgctgga
     2761 tgatggtgtc catgacgcct ccgaagtacc ccgagatgct cccgatgatg acgccgagga
     2821 ggaaggacag gagcacgccc acgaggccga tggagaggga gatgcgcgcg ccgtagatga
     2881 tccgggagaa gatgtcccgc cccagcgtgt cggagccgaa gaggaagagc ggcaccggtc
     2941 cgtccgcccc gaagaggtgg atgtctcccg ggatgatatt ccacatccgg tagggctcgc
     3001 catggacgaa gaaccgaacg cggtgcacct cgtccttgtt gagcgcggtc cccatgcgga
     3061 gcgtcacggg atcccgcttc tgcgtgaacc cgtacatgaa gggctcctgc aggccgtggc
     3121 cggggcggag gatccggatg cgctgcggcg gcgcgctcac gtagttgtcg aaccgggtcc
     3181 gggcgccata cggcgagacg aactcgcaga agatcgccgc caggtacagg atgaccaggc
     3241 ccacgatgga aatggtggcc agccgatgca tgcggaactt gcgccagatg agctgccact
     3301 gcgaggcata gtagagctgc tccgcctggc tctggagctt cctctcacgc cggccccccg
     3361 gcaggatggt catcgccatg agcgttcctc cgggttactg gtatcggatg cgtggatcga
     3421 gccacgccag aaggatatcc gagagcaggg tgccgacgat ggtcagggtg ctcaggatca
     3481 ggaagaatcc tccggccagg tacacgtcct ggtacatgat cgaccgcagg agcaccgggc
     3541 cgctggtagg catgttcagg acgatggaga ccagcac
//
LOCUS       DASRZS010000617         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3049323, whole genome shotgun sequence.
ACCESSION   DASRZS010000617 DASRZS010000000
VERSION     DASRZS010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3049323"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..2627
                     /locus_tag="VFH83_15020"
     CDS             309..2627
                     /locus_tag="VFH83_15020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6487737.1"
                     /translation="MDHQRFRGPGFARHLRNYGGAAAAVLVAFAIAKALELALGPTAL
                     PPFITFYPTVLVVGLAFGWGPALLATAAGGAMAAVWIYPASIGGTASTTEDRLRLVQF
                     LAMGVFMSVVAGLYARARRNALSYERELTLRKRTEAAQQEALGRFSAVLSILYGSVLL
                     VSNEGRVELANRAFCSQFGLPGAPEDLTGLTSEQVIERIRHAYRDPERETRRIAEILE
                     HGEPVRGEEVMMADGRTLLRDFTPISIAGQPFGRLWHQFDITEQRRTQEALRLSEEKF
                     SQAFASNPAAIVLSRLSDGKLLEVNDTWVELTGHRREDVVGGSARTLGVWPTVEEAQR
                     FVRALEEKGFLRGWEQRFFRKSGEAFVAQLSAQILIMNQEKVILSTLVDITERKRFEE
                     RMLQTQKLESLGVLAGGIAHDFNNILQAILGSAELAASQLPPGSAALPNLQVICRSAK
                     RAGDLTRQMLAYSGKGRFQVEPLHLSDVIREMREMLGMSVSKKAVLRYTLAQELPSIR
                     ADLSQIRQVLMNLVINASESLGNQTGTIDIMTGSMDCDGASLGAVWSGEPLKAGTYVV
                     LEVTDTGCGMDEATRLRIFDPFFTTKFTGRGLGLAAVMGIVRGHGGAIRVHSEPGKGT
                     SFRLLFPSFVGAPTSLVRPDRSCQEWQGQGTLLLVDDEAGIRAVGRQMLGYLGFCVLT
                     AADGRQALETLQAHRADITCVILDLTMPSMDGEETFREMRRLAPDLKVILSSGYNEQT
                     VIQRWVGDGLAGFIQKPYELSTLERTLRSILG"
BASE COUNT          470 a          870 c          939 g          447 t
ORIGIN      
        1 cacaacgccg cgggccgagc actaaggggg ttttccgtag actaacccac gccggaccgg
       61 atagcaactc tttttcgctt ggttatcgaa cgccggcctt gtcgcgcgcg tcgacaagga
      121 gacggggccc tggggctacg caacgccacc catcgaagca cacttccagc cgaaaccaga
      181 tgacagcgta gcggccggga tcatgcctcg cgcctcgacg gcgattcgac gccggtcccc
      241 agcgtcagta tgttgtacgg gagccattcg tgagatcgac gcggtccgag aggggaagga
      301 ctgggcgcat ggaccaccaa cgttttcgcg gacctgggtt tgcccgacac ctgcggaact
      361 acggcggggc cgcggccgcg gtcctggtcg cgtttgcgat cgccaaggcg ctggagctgg
      421 cgcttggacc gaccgccctc ccgccgttca tcacgttcta tcctaccgtg ctggtcgtgg
      481 ggttggcctt tggctggggg cccgcgctcc tggcgaccgc ggcgggaggg gcgatggcgg
      541 ccgtctggat ctatcccgcc tcgatcggag ggacggcgtc gaccacggaa gaccggctcc
      601 ggctggtcca attcctcgcc atgggcgtgt tcatgagcgt cgtcgccggg ctctacgcgc
      661 gcgcccgacg gaatgccctg tcctacgagc gggagctgac gctccgaaag cggaccgagg
      721 cagcgcagca ggaggcgctc ggccgcttct ccgcggtcct gtcgatcctg tacggcagcg
      781 tgctcctggt gtcgaacgaa gggcgggtgg agctggcaaa ccgcgccttc tgctcccagt
      841 tcggcctgcc gggggcgccg gaggacctga cggggctgac ctcggagcag gtgatcgagc
      901 ggatccgtca cgcctatcgg gatccggagc gggagacccg aaggatcgcg gagatcctgg
      961 agcacggcga gccggtccgg ggagaggaag tcatgatggc ggacggccgg acgctcctac
     1021 gggacttcac ccccatctcc atagcgggcc agccgttcgg ccggttgtgg caccagttcg
     1081 acatcaccga gcagcgcagg acgcaggagg ccctccggct ttcggaggaa aagttctccc
     1141 aggcgtttgc cagcaacccg gcagccatcg ttctgagccg gttgagcgac gggaagctgc
     1201 tggaggtgaa cgatacctgg gtcgagctca ccggccaccg acgggaagac gtcgtggggg
     1261 ggtcagccag gaccctggga gtctggccca cggtcgaaga ggcgcagcgg ttcgtccggg
     1321 cgctggagga aaagggcttc ctgcgagggt gggagcagcg gtttttcagg aagtccggcg
     1381 aggccttcgt ggcgcagctc tccgcgcaga tcctgatcat gaaccaggag aaagtgatcc
     1441 tctccaccct ggtggacatc accgagcgaa aacgcttcga agagcggatg ctgcagaccc
     1501 agaagctgga gagcctgggt gtgctggcgg gcgggatagc gcacgacttc aacaacatcc
     1561 tccaggccat cctgggaagc gcggagcttg ccgcctctca gctccccccc ggctcagcag
     1621 ctctcccgaa cctccaggtg atctgccggt ccgcgaagcg agcgggtgac ctgacccgac
     1681 agatgcttgc ctactcggga aaaggacgat tccaggtgga gccgctccat ctctcggacg
     1741 tgatccggga gatgcgggag atgctgggaa tgtcggtctc caagaaggcg gtgcttcggt
     1801 acacgctggc ccaggagctg ccctccatcc gggccgacct ctcccagatc cgccaggtgc
     1861 tcatgaacct ggtcatcaac gcgtcggagt cccttggcaa ccagaccggg accatcgaca
     1921 tcatgaccgg gtcgatggac tgtgacgggg cctcgttggg cgccgtctgg agcggagagc
     1981 ccctcaaggc cggcacgtac gtggtcctgg aagtgacgga taccggctgc gggatggacg
     2041 aggccacgcg gcttcggatc ttcgacccgt tcttcaccac caagttcacc ggcagaggac
     2101 tgggtcttgc cgccgtcatg gggatcgtgc gcggccacgg cggcgccatc cgcgtgcaca
     2161 gcgagccggg caagggtacc agcttccggc tgctcttccc gtcgttcgtc ggggcgccaa
     2221 cgtccctggt ccgcccggac cgctcctgtc aggaatggca ggggcaaggg acgcttctcc
     2281 tggtggacga cgaggcgggc attcgagccg tgggccgaca gatgctcggg tatctgggat
     2341 tctgcgtgct caccgcggcg gatggccggc aggctctgga gaccctccag gctcaccggg
     2401 cggacatcac ctgcgtcatc ctcgacctca ccatgccgtc catggacggc gaggagacgt
     2461 ttcgggagat gcgcagactg gcgccggacc tcaaggtgat cctgtccagc ggctacaacg
     2521 agcagacggt gatccagcga tgggtcgggg acggccttgc gggattcatt cagaagccgt
     2581 atgagctttc gaccctcgag cgaaccctga ggagtatcct gggctgaggg tcgaagcgcc
     2641 gcttccccgg acgctctccc accgagcccc ggagcgaagt caacagaccc ggacgcattc
     2701 gatccccagg agacgggcca gcttct
//
LOCUS       DASRZS010000618        11540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1892670, whole genome shotgun sequence.
ACCESSION   DASRZS010000618 DASRZS010000000
VERSION     DASRZS010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11540
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892670"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VFH83_15025"
     CDS             complement(<1..826)
                     /locus_tag="VFH83_15025"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET6487738.1"
                     /translation="MTRVSLASSVSSRVDFESNPQGEITLTGFAQNVRTLSWGAFLIL
                     RLPNHLLQVVISKDMVALGDIPVESTVRATGKLKPAALKDKALNPANVELEASRIEIL
                     SGPADGPLPLDTSKKELHADQNTIFDLRPLSLRHPKERAVFRIQSAIFNEFGNHLTRI
                     GFTRICSPKLVFSGAEGGANVFKLDYFGRTAYLAQSPQFYKQMMVGVFGRVFEEAPAF
                     RAEKHNTSRHVNEYISLDFEMQLDSGFLDIIQVEASVLRSILAHLKEGYAPELALLE"
     gene            1011..1229
                     /locus_tag="VFH83_15030"
     CDS             1011..1229
                     /locus_tag="VFH83_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487739.1"
                     /translation="MSNEALILDLVEWVAAHPREYSEVMDAWRTSCPRLTIWEDAVDA
                     GLVAREGTVVRATSQGIELLRARRGAAV"
     gene            complement(1226..2089)
                     /locus_tag="VFH83_15035"
     CDS             complement(1226..2089)
                     /locus_tag="VFH83_15035"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET6487740.1"
                     /translation="MTGPFDPDRLSLIARGGQADIYDAGDGKVLRLARRPQDFDRIRY
                     EYEVYACLGAAGLPVPRAYELLEVRGAPAILMDRVDGGSLMARIRGNPLTARAVAREL
                     AQLHLQILGHEAPSLLTRAKAKSEFCIRGSSRLSEDSKKRLLSLLDGLPEGTAVCHGD
                     FHPGNVLRQRGKSYIIDWSAASRGDFHADVAHSFLLMRVVPKVPGTRAMMHLLQRSIG
                     RAIASTYLSTVGKDRPLDRRRLSKWVLLNAAERTYHGLPSEQDTLGRFIEKGLALLAE
                     NPNQEHLHELI"
     gene            complement(2086..2538)
                     /locus_tag="VFH83_15040"
     CDS             complement(2086..2538)
                     /locus_tag="VFH83_15040"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6487741.1"
                     /translation="MAGDGAATLGRAEPSAAPAIASLVRELAREAGGQSPITEEYVRA
                     YLASPGCGILLARRGELVVGMISYSIRAGLYHAAPSCVIEDLIVVKDARNQGIGGLLL
                     SDVVAMAERLGCAEVSVSTETDNVGAVRFYRRHGLSDESVYLEKHFGP"
     gene            complement(2541..3083)
                     /locus_tag="VFH83_15045"
     CDS             complement(2541..3083)
                     /locus_tag="VFH83_15045"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HET6487742.1"
                     /translation="MVTSLPVEASLQYPIGNLVFVEPNPALRRTWIEHYREAPGLLRR
                     AVDGLDSAQLQTPYRPGGWTVAQVVHHMAEMDASAYTRLKFAMTEDAPTVAAAKQALW
                     AEVPDSRTSTIEHSLALFEAVRVRWVDAWEALREEDFKRQWKHYRLGLVTLDHLLQQY
                     AWHARHHTAHITGLRARMGW"
     gene            complement(3089..4204)
                     /locus_tag="VFH83_15050"
     CDS             complement(3089..4204)
                     /locus_tag="VFH83_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487743.1"
                     /translation="MGNGTRLFCVIVGIALLGGCVSQKPSVEGLEPVPGENLDLRGFN
                     LGAWDKLGLSARNPVNEAISFAAKEGASFLAIDWRVYFADDGGLAGSWDKDVPQENDL
                     IAAIAKAKEAGMYVLLKPHVAGPSGGNRNNMDSDPRTFRASNFFRDWGAYLVSLAKSM
                     EGQRADAICIGTELSFLDGASSREWHDLIASIRAVYPGELTYDAVFSVWDKWNFMKDV
                     VFWDALDYVGCSVYLPVTADDSASVEQIEEGWRSNRIGDTPDIPSRLQAMGRRVGKKV
                     MVLEAGYPSRSGGLLNPSDPPSRDSAVDNDLQARGLAAMLEVLYGNGGTWLKGISLWD
                     LTPGLMGPDTVSDIWRTQGWSVYGKPAAQVVKGYFSR"
     gene            4327..5265
                     /locus_tag="VFH83_15055"
     CDS             4327..5265
                     /locus_tag="VFH83_15055"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HET6487744.1"
                     /translation="MANFVFVPAWVVQDLIVYLVGILAVGFILKKEPHPQSILMEFLC
                     FTFLYAAVYENFATRMGWYAYGPSLLMIANVPLSIPIIEYLVVYATLRLLGFTEMPAW
                     CKPIIVGFVAMLFDFSLDPIAVKLIHSVNGGIIGRWSWYPRGSDVQIYGEPVYNFTGW
                     VLICGYAASLLLAGRLWFRKSGYRRLVGILYPLLCMIGALGLLITPLSQLLLWLQPAY
                     AKGSVGEWIMLGFWLVLPTAILLVFWRGRMKGRFSLRENWIVATVLIGFPALNVITTL
                     AAGVFDILWLVALCAVAEAILVLAVRLRGRTLAFHG"
     gene            complement(5287..6390)
                     /locus_tag="VFH83_15060"
     CDS             complement(5287..6390)
                     /locus_tag="VFH83_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487745.1"
                     /translation="MRDDVTEALEPMTNALLRKLGPEVDLIFRYGSTLKGTTHVYSDL
                     DVSYVPVHESTGDSITVLVADVLIDLYPIHWSQLESMADFENVSASVLLENQLIYHRN
                     EAVVDRLRSLASKLRSNLEPSARAPMLQTAQRLFQRIGYDFYLLREQAAAGHRLSCLY
                     QASKITRAVFHCLAACNQACVDTRRVDRVLALPKLPVDFATTVQSISNATQPQEILGA
                     CERLMSSTRALLLREQRSMPAGDATFPSVFRAAYPELKGSIQHAKLASDRGDRLDPSL
                     VSLYHEVMVHMAQATTGVSYGSFSPIADYEQDLTALGFPDLMGAFVAGDPKPLGEPCD
                     VFDERLKEYLQQHGVGLNAFGSVEDLRTHLETD"
     gene            complement(6445..7002)
                     /locus_tag="VFH83_15065"
     CDS             complement(6445..7002)
                     /locus_tag="VFH83_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014058635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET6487746.1"
                     /translation="MRDVIYLMNLSLDGFIEGPDGKFEWTHPDEEIHRFHNRTADEMG
                     AFLYGRRLYETMAVWQTWGDDASLPDYVREFARVWRSKPKIVFSTTLTAVGEGCRLVR
                     GEVGAEVSRLKRESGGDLSVGGPGLASALARLDLIDEYRLVIYPILVGGGKSYYPPLA
                     GPVPLRLLETRTFARGAVYLRYRRA"
     gene            complement(7021..7458)
                     /locus_tag="VFH83_15070"
     CDS             complement(7021..7458)
                     /locus_tag="VFH83_15070"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6487747.1"
                     /translation="METGTTFTVEEEASRENVEAIRQGVHSYNERFAERDNHRFLTVL
                     ARDRDGRIVGGLTGGTYWRWLHIDYLWVESASRRHGIGRGLMLEAEREALRRRCRHAH
                     VETHDFQAPEFYRKSGYVEFARLEDLPPGHTKIFLRKDLSASV"
     gene            complement(7472..8491)
                     /locus_tag="VFH83_15075"
     CDS             complement(7472..8491)
                     /locus_tag="VFH83_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HET6487748.1"
                     /translation="MVELIKPRKLRAGDTVATVSISGGRAGDPDMIGRYRLGKRRLQE
                     EFGLEVVETPNAMKGNDFIYRNPKARVEDLHGALLDPAVKGIIANHGGDDSYRLLPYV
                     DFDILHDHPKAFVGFSDISTTHNLFTYAGVTSYYGPALLTPIAQPVRLDRYTAFWMKK
                     VLFSSEPIGRVDACESWTPITWNDDPNQEIPWTQNGGYEVLQGTGTVAGRLLGGCSAP
                     LQQIMGTFLFPTPEQWRDSIIFLEIGSSYRLQLAGLHLLRSLAATGMFRRAKGLICQS
                     MTEEEKQTLLKVLRDEEGLTDLPILIGVEFGHRTPMTVLPIGAMAEIDCDAGTFTILE
                     SGVLD"
     gene            complement(8746..9639)
                     /locus_tag="VFH83_15080"
     CDS             complement(8746..9639)
                     /locus_tag="VFH83_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487749.1"
                     /translation="MMKNGLPLLAICVLILVATGIGLFYHPDVPPIRTESLRGQSATF
                     VAAGPYRYNPVFFAREGAVWDAVNLFLALPLLVLSALFALRDNLRGRLLLGGMLAYFW
                     YVYLGTALMNAFNNLFLVYVGIFALCAVAFIQNAARIDVDRVRAACADRFPRRILIGY
                     SLGIAAVLVGLWTSRVAAVMRTGMFPPEYAGINTLGSQALDLGLLVPLALSTAVLLGK
                     RSPWGYYLASITVSMGFMMSVTIPCWIVVPLVQDGQARMGEAVPFFVVSLVGITVAVL
                     YLSRLRPASRARTAAAMPVGR"
     gene            complement(9700..10113)
                     /locus_tag="VFH83_15085"
     CDS             complement(9700..10113)
                     /locus_tag="VFH83_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6487750.1"
                     /translation="MGTSIFFVDRSHRILLVLRDDTKGLPFPDCWDMPGGHVEPGETP
                     VQCICREMNEEMGLHLEDPSLFTVVDMPDRIECCFWQRADFDTLRIRLTEGQRIQWFS
                     EAEVARIPDHKLAFGFRPIITDFFRLRPFDRGPAP"
     gene            complement(10164..11018)
                     /locus_tag="VFH83_15090"
     CDS             complement(10164..11018)
                     /locus_tag="VFH83_15090"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HET6487751.1"
                     /translation="MGYPQGFDPRKAVQLAELVTQAYQQFDSFRRDRPWSIGDGYALI
                     RELRYAEGEAASRRAVPTQFERELRQLARPRSPRRDGLPIGFLARRGAEAFLVFRGTM
                     TSNEWIADLKVRLVPCPFPGSGKVHDGFLRTYELFRATILDAVGATEGVGRFYVTGHS
                     LGAGLATLAAFDIATLQRPFSLIAYTYASPRVGDRQFASDYNARLGLRSYRVANTCDM
                     VVSVPFPVPFLGFLGGYFTHVDAPVDFTVQREDVEKNHDIGTYLDAMKRLGPRQSILG
                     RFFRGQKR"
     gene            complement(11044..>11540)
                     /locus_tag="VFH83_15095"
     CDS             complement(11044..>11540)
                     /locus_tag="VFH83_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487752.1"
                     /translation="GIEVRNLKVSPRDVSMAERLPYLRLPERPDLQKRRKGIRDLRET
                     FGVPTRTATALYEYNNADPVCIAKADSGDCLFIREGLCAQYAVRPLVCRLYHCRLGEK
                     LSALNEAIIGQGVWHSYCALGWIDEKEIAHNPFVGASDYQRIRLDAFDRDFEDLVAQA
                     SYLL"
BASE COUNT         2078 a         3747 c         3635 g         2080 t
ORIGIN      
        1 cctccagcag ggccagctcc ggcgcgtacc cttctttcag atgcgccagg atggatcgca
       61 gcacgctggc ctccacctgg atgatgtcca ggaagccgct gtcaagctgc atctcgaagt
      121 ccagcgagat gtactcgttc acgtgtcggg acgtgttgtg cttctccgcc cgaaacgcgg
      181 gcgcctcctc gaagacccga ccgaatacgc ccaccatcat ctgtttgtag aactggggtg
      241 attgggccag gtaggcggtc ctgccgaagt agtccagctt gaagacgttg gccccgccct
      301 cggcgccgga gaacaccagc ttgggagagc agatccgcgt gaagccgatc cgggtcaggt
      361 gatttccgaa ctcgttgaaa atggccgact ggatgcgaaa gacggcccgc tccttgggat
      421 ggcgcagcga cagggggcgc agatcgaaga tcgtattctg gtccgcgtgg agctccttct
      481 tcgaagtgtc cagcggcagg ggaccgtcag cgggtccgga gaggatttcg atccggctcg
      541 cttccagctc gacgttggcg ggattgagcg ccttgtcctt caaggccgcc ggcttgagct
      601 tgcccgtcgc gcgcacggtc gactccaccg gtatgtcccc gagcgcgacc atgtccttgc
      661 tgatcaccac ctgcagcagg tggttgggca ggcggaggat gaggaacgcg ccccaggaaa
      721 gcgttcgcac gttctgagcg aagccggtga gggtgatctc gccctgggga ttgctttcga
      781 agtcgacccg gctggaaacg gagctcgcca gcgatactcg cgtcacggca taccttcctg
      841 gaccgcagtg tatcggcgat gcgatccctg acgcaaggca gggtggacag ggccgccggg
      901 cggccgggca ggcgccgcag actccgggcg ccacgccgcc gacggtgggc cgtaccgggg
      961 ctcgagcttc ccaaccgccg cgggtctcag tacctttggc gcggtctccg atgtcgaacg
     1021 aagcgcttat cctggatctc gtcgaatggg tcgcagcgca ccccagggag tattccgagg
     1081 tgatggatgc ctggcgcacg agctgtcccc ggctcacgat ctgggaagat gcggtggatg
     1141 ccggcctggt ggcgcgcgag gggacggtgg tccgggcgac ctcgcaggga atcgagctcc
     1201 tgcgcgcgcg ccggggcgcc gcggtctaga tcagctcgtg gagatgctcc tggttcgggt
     1261 tttccgccag gagcgccagt cccttttcga tgaaccgccc caacgtgtcc tgctccgaag
     1321 gcagtccgtg ataggtccgc tccgctgcat tcagcaacac ccacttcgac aaccgtcggc
     1381 ggtccagggg acggtctttc cccacggtcg aaaggtacgt agaggcgatt gcccggccga
     1441 tgctgcgctg aaggagatgc atcattgccc tcgtgccggg gaccttcggc accaccctca
     1501 tcaggaggaa gctgtgagcc acatccgcgt ggaagtcccc tcgggatgcg gctgaccagt
     1561 cgatgatgta gctcttccca cgctgacgca ggacattgcc tgggtggaaa tcgccgtggc
     1621 aaaccgcagt gccctccgga agcccgtcga gcagggacag cagccgcttc ttgctgtcct
     1681 cggacagtct tgaggaaccc cggatacaga actcggactt tgccttggcg cgcgtcagga
     1741 gggatggggc ctcgtgtccc aggatctgta gatggagctg cgcaagctcc cgcgccacgg
     1801 cccgcgcggt cagggggttc ccgcggatcc gcgccataag ggagccgccg tccacccgat
     1861 ccatcaggat ggcgggagct ccgcggacct ccaggagctc atacgctcgc ggcacgggaa
     1921 gccccgcggc gcccaggcag gcgtacactt cgtattcgta tcggatgcgg tcgaagtcct
     1981 gaggacgccg ggccaatcgc agaactttcc cgtcgccggc gtcgtagatg tccgcctgcc
     2041 cgccgcgggc tatcagggaa aggcggtccg gatcgaaggg gcccgtcatg gtccgaagtg
     2101 cttctcgagg taaacgctct cgtcggagag accgtgccgt cgatagaacc gaacggctcc
     2161 cacgttgtcc gtctccgtgc tgacggagac ctccgcgcag cccagcctct cggccattgc
     2221 cacgacatca gagaggagca gaccgccgat cccttggttg cgggcgtcct tgacgacgat
     2281 caggtcctcg atgacgcagg atggcgccgc gtgatacaga ccggcgcgga tcgagtagct
     2341 gatcatcccg acaaccagct caccccgtct ggccagcagg atcccgcacc cggggcttgc
     2401 caggtaggca cgaacgtact cttcggtgat ggggctctgc ccacccgcct cccgggcaag
     2461 ctccctcacc agggaggcga tggcaggagc cgccgatggc tccgcccggc cgagcgttgc
     2521 cgcgccgtct ccggccatgg tcaccagccc atccgggccc gcaggcccgt gatatgcgcc
     2581 gtgtggtggc gagcgtgcca cgcgtactgc tggaggaggt ggtcgagggt taccagcccc
     2641 agacggtagt gcttccattg ccgcttgaaa tcctcttccc gcagcgcctc ccaggcgtcg
     2701 acccaccgca cccgtacggc ttcgaagagc gccaacgagt gctcgatcgt ggaagtcctg
     2761 gagtcgggca cctctgccca gagcgcctgc ttggccgcgg ccacggtcgg agcgtcctcg
     2821 gtcatggcga acttcagccg cgtgtaagcg ctggcatcca tctcggccat gtgatgtacg
     2881 acctgcgcca cggtccagcc gccaggacga tagggagtct ggagttgcgc ggaatccagg
     2941 ccgtcgacgg cccggcgcag aaggcccggt gcctcccggt agtgttcgat ccacgtgcgt
     3001 cgcaacgcgg gatttggttc cacgaacacg aggttgccga tggggtactg aaggctggcc
     3061 tccacgggga gtgaggttac cacggtttct accgggagaa gtagcccttc acgacctgcg
     3121 cggccggctt gccatagacg gaccaccctt gggttcgcca gatgtccgag acggtgtcag
     3181 gacccatcaa ccccggcgta agatcccaca ggctgatacc cttcagccag gtcccaccgt
     3241 ttccgtacag gacctccagc atggcggcca ggccgcgagc ctgcaggtca ttgtccacgg
     3301 cggagtctct ggacggaggg tcggaaggat tcagaagccc accggaccgg ctcgggtagc
     3361 ctgcctccag caccatgacc ttctttccga cacgacgacc catcgcctgg agcctggaag
     3421 gaatatcggg agtatcgccg atgcggttcg accgccatcc ctcctcgatc tgctccacgc
     3481 tcgccgagtc gtccgcggtg accggcagat agacggagca gcccacgtag tccagcgcgt
     3541 cccagaacac gacatccttc atgaagttcc acttgtccca gaccgagaag accgcgtcgt
     3601 acgtcaactc tccggggtag acggcgcgga tcgaggcaat caggtcatgc cattcgcggg
     3661 agctcgcgcc atccaagaag ctgagctcgg tcccgatgca gatggcgtcg gcacgctgcc
     3721 cttccatgga cttcgcgagg gagaccaggt aggctcccca gtctcgaaag aagttgctgg
     3781 cccggaacgt tcgcggatcg gagtccatgt tgtttcggtt cccgccggac gggccggcga
     3841 catgcggctt caggagcacg tacattccgg cctccttggc cttggcgatt gcggcaatca
     3901 ggtcgttctc ctgggggaca tccttgtccc aggaaccggc aaggcctcca tcgtccgcga
     3961 agtacacccg ccagtcgatg gcaaggaagc tcgcgccttc cttggcggca aaggagatgg
     4021 cttcattcac cgggtttctg gccgatagtc ccagcttgtc ccaggcgccg aggttgaacc
     4081 cccgaagatc aaggttctcg ccgggtacag gctcaagacc ctcgaccgaa ggtttctggc
     4141 tgacgcagcc gccgagcaga gcgatcccaa cgatcacgca gaacagtctt gttccgttcc
     4201 ccatgcctgg cctcactttt cgtgtggatt cttgacatcg ctgatacact gattataatc
     4261 agaccgaccg gtcggtttat ggagccggag cgttcggttc gacgggccgg cgcaaaggag
     4321 ccccaagtgg ccaacttcgt gtttgtaccc gcatgggtcg tccaggatct catcgtctac
     4381 ctcgtcggca tccttgcggt aggattcatc ctcaagaaag agccgcatcc gcagtccatc
     4441 ctcatggagt tcctctgctt cacgttcctc tatgcggcgg tctacgagaa cttcgccacc
     4501 cgaatgggct ggtatgcgta tggaccgtct ctcctgatga tcgcaaacgt gcccctctcc
     4561 atccccatca tcgagtacct cgtggtctac gccacccttc ggctcctggg gttcacggag
     4621 atgcccgcct ggtgcaagcc gatcatcgtg ggattcgtgg cgatgctctt cgacttctcc
     4681 ctggacccca ttgccgtgaa gcttatccat tcggtgaacg gtgggatcat cggccgatgg
     4741 agctggtacc cccgtggttc ggatgtccag atctacggtg aaccggtgta caacttcacc
     4801 ggctgggtcc tcatctgcgg atacgccgcg tccttgctcc tggcgggacg cctgtggttt
     4861 cggaagtccg gctaccggcg gctggtgggg atcctctatc cgctcctgtg catgatcggc
     4921 gccctcgggc tgctgatcac tccgttgagc caactccttc tctggctgca acccgcctat
     4981 gccaaaggga gcgtcggcga gtggatcatg ctgggattct ggctggtcct gccgaccgca
     5041 atcctgctgg tcttctggag gggccgaatg aaaggccgat tctccctgcg ggagaactgg
     5101 atcgttgcga ccgtgctcat cggcttcccg gccctcaacg tcatcaccac gctcgcggcg
     5161 ggtgtcttcg acatcctctg gctggtggcg ctgtgcgcgg tggcggaagc gatcctcgtc
     5221 ctcgccgtcc ggcttcgagg gagaacactc gcgtttcacg gctgattcct tcccggcccg
     5281 gactcctcag tccgtctcga ggtgggtccg aagatcctcc acgctcccga aggcgttcag
     5341 cccgacgccg tgctgctgaa ggtattcctt caggcgctcg tcgaagacgt cgcacggctc
     5401 gccgagaggc ttcggatcac cggccacgaa ggctcccatc aggtcgggaa acccgagcgc
     5461 ggtcaggtcc tgctcgtagt cggcaatcgg gctgaagctc ccgtacgaga cccccgtggt
     5521 cgcctgggcc atgtggacca tcacctcgtg atagagagat accagactgg gatcgagccg
     5581 atcgccacga tcgctggcca gcttcgcatg ctggatgctc cccttcagct cggggtaggc
     5641 cgcacggaat acgctcggaa aggttgcgtc accggcgggc atcgaccgct gctcacggag
     5701 aaggagcgcc cgcgtgctgc tcatcagccg ctcacaggcg cccaggattt cctggggctg
     5761 ggttgcgttg gagatcgatt ggacggtcgt ggcaaaatcg acgggcagct tgggaagcgc
     5821 caggacccga tcgaccctac gggtatcgac gcacgcctgg ttgcacgccg ccagacagtg
     5881 gaataccgcc ctggtgatct tgctggcctg gtacagacag gagagccggt gccccgcggc
     5941 agcctgctcc cgcaacaggt agaagtcgta tccgatccgc tgaaagaggc gctgcgccgt
     6001 ctgcagcatc ggagcccggg cggaaggctc gaggttggat ctgagcttgg acgcgagaga
     6061 acggaggcga tcgacgaccg cttcgttgcg gtgatagatg agctgattct ccaggaggac
     6121 gcttgcgctc acgttctcga agtcggccat ggactcgagc tgcgaccagt ggatgggata
     6181 caggtcgatg aggacgtcgg cgaccaggac cgtgatgctg tctcccgtgg actcgtgaac
     6241 gggaacgtaa gagacgtcga gatcggagta gacgtgcgtg gtccccttga gggtcgagcc
     6301 gtatcgaaag atgaggtcca cctcgggccc cagcttccgc agcagggcgt tggtcatggg
     6361 ttccaaggct tcggtgacgt cgtcgcgcat gaggcttcct ctcgtcgctg gggattcctg
     6421 ccccagggca gcgtttccca cccctcaggc gcgccgatag cgcaggtata ccgcgccacg
     6481 ggcgaaggtt ctggtctcca ggaggcggag cgggacagga ccagccagcg gcgggtagta
     6541 cgacttgccg ccgccgacca ggatcggata gatgacgaga cgatactcgt cgatcaagtc
     6601 gagtctggca agagctgagg ccaggcccgg tccgcccacg ctcaggtccc cgccgctctc
     6661 tcgcttcagc cgagagacct ccgcgccaac ctctccccgc accaatcgac agccctcacc
     6721 tacggccgtc agcgtggtcg agaacacgat cttgggcttg ctcctccaga cgcgggcaaa
     6781 ctcccgcacg tagtcgggga gggaagcgtc gtccccccag gtctgccaga cagccatggt
     6841 ctcgtacagc cgtcggccgt agaggaaagc gcccatttcg tcggccgtgc ggttgtgaaa
     6901 ccgatggatc tcctcgtccg gatgggtcca ctcgaacttc ccgtccggcc cctcgatgaa
     6961 gccgtccagc gacaggttca tcaggtagat gacgtcgcgc acggggtctc ctctcgacct
     7021 ctacaccgac gcgctcagat cctttctgag gaagatcttc gtgtgtcccg gcgggaggtc
     7081 ttccaggcgc gcgaactcca cgtacccgct tttccggtag aactccggtg cctggaagtc
     7141 gtgggtctcc acatgggcat gccggcagcg ccgtctcaag gcttctcgct ccgcctccag
     7201 catcaggcct ctgccgatcc cgtggcgacg gctcgcgctc tcgacccaaa gatagtcaat
     7261 atggagccag cgccaatagg tcccgcccgt cagtcccccg acgatccgcc cgtctctgtc
     7321 tcgagccagc accgtcagaa agcgatggtt gtccctctcc gcgaaccgct cgttgtagga
     7381 atggactccc tgccggatcg cctcgacgtt ctcccgcgat gcctcttctt caacggtgaa
     7441 ggtcgttccc gtctccacgg agcccccctt ctcagtccag taccccggat tccaggatcg
     7501 tgaaggtccc ggcatcacaa tcgatctccg ccatcgcccc gatgggcaga acggtcatgg
     7561 gcgtccggtg gccgaactcc acgccaatca gaatgggcag atccgtcaag ccctcttcgt
     7621 cccgcaggac cttcaggagc gtctgcttct cctcttccgt catgctttga cagatcagcc
     7681 ctttcgcccg gcgaaacatc cccgttgccg cgagggacct caggagatgc aatcccgcga
     7741 gctgcaagcg ataggacgag ccgatctcca ggaagatgat gctgtcacgc cactgttcgg
     7801 gcgtcgggaa caggaaggtg cccatgatct gttgaagcgg cgcggaacag ccacccagaa
     7861 gccgccctgc caccgttccg gtaccctgca ggacctcgta cccgccgttc tgggtccagg
     7921 ggatctcctg gttcgggtcg tcgttccagg tgatgggcgt ccaggattcg cacgcatcta
     7981 cccgaccgat cggttcactg gagaacagca ccttcttcat ccagaaagcc gtgtaccggt
     8041 ccagccgtac cggctgggca atcggggtca gcagcgccgg cccatagtac gacgtcacgc
     8101 cggcataggt gaacaggttg tgcgtcgtcg agatatccga gaatcccacg aaggccttcg
     8161 gatggtcgtg gaggatatcg aagtccacgt agggcaggag ccggtaggag tcgtcgccgc
     8221 catggttcgc gatgatccct ttcacggccg ggtccaggag cgccccgtgc agatcctcca
     8281 ccctggcttt cggattccga tagatgaagt cattgccctt catggcgttg ggcgtttcca
     8341 ccacctcaag cccgaactct tcctggagcc gtcgcttgcc cagccggtac cgtccgatca
     8401 tgtcagggtc tccggcccgc cctccggaga tggacaccgt ggcaacggtg tcaccggcgc
     8461 gcagcttccg tggcttgatg agctcgacca tgccgagacc tccttctgtg ccgaccccgg
     8521 tgacctgagg caaaggcgcg tcttctcccg attgcggtag tccgtgaagg ggaacacctg
     8581 cccgtccgca gtggaccagt actctgcgcc attgtcgtgc catctcgcgg cacggcctgc
     8641 gcgtcgccca ggtacgttct gatcatcacg cgacatcctt cgagcccccg gggcaccccc
     8701 tggaggggcc ttgccgcttc agcgctgagc cttccggtag ccgcctcagc gcccaaccgg
     8761 catcgcagcc gcggtccttg cgcgggaagc cggcctcagc ctggaaagat acaggaccgc
     8821 cacggtgatg ccaaccaggg agacaacgaa gaacggaacc gcctccccca tccgcgcctg
     8881 cccgtcctgg accaggggca ccacgatcca gcaggggatg gtaacagaca tcatgaaccc
     8941 catcgacacg gtgatggagg caaggtagta gccccatggg gatctcttcc ccagcagcac
     9001 cgccgtagag agagccaggg gcaccagcag cccgagatcg agtgcctggc tgcccagcgt
     9061 gttgatcccg gcgtactccg gcgggaacat cccggttcgc atgacggctg ccacgcggga
     9121 cgtccacaag cccaccagga ccgcggcgat ccccagggag tagccgatga ggatgcgccg
     9181 ggggaaccga tcggcgcatg cggcgcgcac acggtccacg tcgatcctcg cggcgttctg
     9241 gatgaaggct accgcgcaga gagcgaagat ccctacatag accaggaaca ggttattgaa
     9301 ggcgttcatc agggcagtgc cgaggtagac gtaccagaag taggcaagca tccctcccag
     9361 caggagcctg ccccgcagat tgtctcgaag cgcgaacaag gcagaaagca ccaggagcgg
     9421 tagagccagg aagaggttga ccgcgtccca cacggcaccc tcacgcgcga agaacaccgg
     9481 gttgtaacgg tagggaccag cagctacgaa tgtcgcgctc tggccccgaa gactctcggt
     9541 ccggatgggc gggacatccg gatggtagaa cagaccgata ccggtggcaa cgagaatcag
     9601 aacgcagata gccagaagag gcagaccgtt cttcatcatg gtatctccga ggatgcaaca
     9661 ccgcgatgcc ggcgtcctgc caccgccggg ggagcagcgt cagggcgccg ggccacggtc
     9721 gaacggccgc aatcggaaga agtccgtgat gatggggcga aagccgaacg caagcttgtg
     9781 gtccgggatc cgggccacct cggcttcgga gaaccactgg atcctctgtc cctcggtcag
     9841 ccggatccgt agcgtgtcga agtcggcccg ttgccagaag cagcactcga tccggtcggg
     9901 catgtcgacc acggtgaaca gactggggtc ctccaggtgc aggcccatct cctcgttcat
     9961 ctcccgacag atgcactgca ccggcgtctc tcccggttcg acgtgtcctc cgggcatgtc
    10021 ccagcagtct ggaaacggaa ggcccttcgt gtcgtcgcgg agaaccagga ggatccggtg
    10081 ggagcggtcg acaaagaaga tgctggtacc cattgccttc ggggtcctgg ggcgcgccag
    10141 caaggacgcg agcgttggct tcctcatctc ttctgcccgc ggaagaaacg tccgaggatg
    10201 ctttgccgag ggccgagtct cttcatggcg tcgaggtatg tcccaatgtc atggttcttc
    10261 tccacgtcct ccctctgaac cgtgaagtct accggcgcat cgacgtgggt gaaatagccg
    10321 ccgaggaatc ccaggaacgg tacggggaag gggaccgaca ccaccatgtc gcaggtgttg
    10381 gcaacgcgat aggaccgcaa ccccagtcgg gcgttgtagt cggaggcaaa ctgacggtcc
    10441 ccgacgcggg gagatgcgta ggtgtaggcg atcagggaga acggccgctg cagggtcgcg
    10501 atgtcgaaag ccgccagcgt ggccagtccg gctccaaggc tgtggccagt gacataaaac
    10561 cggcccaccc cttcggtggc ccccacggca tccaggatgg tcgctcggaa gagctcgtaa
    10621 gtgcggagga acccgtcgtg caccttgccg gatcccggga atgggcaggg aacgaggcgg
    10681 accttgaggt ccgcgatcca ttcgttgctg gtcatggtgc ctcggaagac gagaaatgcc
    10741 tcggcgcccc gccgcgccag gaacccgatg ggcagcccgt ccctccgcgg cgaccttggc
    10801 cgagcgagct gccgcagctc gcgttcgaac tgcgtcggga ccgcccttcg ggaagctgcc
    10861 tccccctcgg cataccgcag ctcccggatc agcgcatatc cgtcgccgat gctccaggga
    10921 cggtcccgtc ggaacgagtc gaactgttgg taggcctgcg tgacaagctc ggcgagctgg
    10981 accgccttcc gtgggtcaaa gccttgcgga tagcccagag atcacctccc gatcgagagc
    11041 cgctcagagc aggtaggaag cctgcgcgac aaggtcttcg aagtcacgat cgaaggcatc
    11101 cagccggatc cgttggtagt cgctggcgcc tacgaacggg ttgtgggcga tctccttctc
    11161 gtcgatccat ccgagcgcgc agtaggaatg ccagacgcct tgaccgatga tcgcctcgtt
    11221 cagagcggac agcttctccc ccaaccgaca gtgatagagc cggcagacca gcggcctcac
    11281 ggcgtactgc gcacacagac cctcccggat gaataggcag tccccggagt ccgccttcgc
    11341 gatgcagacc ggatcggcgt tgttgtattc gtagagcgcg gtcgccgtcc tcgtgggcac
    11401 cccaaacgtc tcccgcagat cgcggatacc ttttcggcgc ttctggagat ccggtcgctc
    11461 cggcaaacgg agatagggca accgttcggc catcgagaca tctcggggag ataccttgag
    11521 gttcctgacc tcgataccgt
//
LOCUS       DASRZS010000619         4976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5731950, whole genome shotgun sequence.
ACCESSION   DASRZS010000619 DASRZS010000000
VERSION     DASRZS010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5731950"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VFH83_15100"
     CDS             <1..929
                     /locus_tag="VFH83_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HET6487753.1"
                     /translation="LAALDHGAMHVQGTINGWGERCGNLNLCTLIPTLCLKRQDSAGV
                     CVNLKLLTALSRFVAEKANIIPDKRWPYVGETAFSHKAGQHADVVSKAARLMEHVDGA
                     LVGNERRILLSELAGKSTIVRKMERYGDFAKSAEVVSGLLEQLKLLEEKGYEYEAAEG
                     SFDLLIRRALGRYRPLLELSNYHLENYKAGATPAKTVGRLFVRVNGDTLMGAAVGDGP
                     VDTLNGALRDALLPLHPFLQKLKLTDYRVRVLNPERATAAKVRVFITTSDGDQTWDTV
                     GVDENIIEASWQALIDGVDYYFNNFVLEEKTG"
     gene            complement(940..2025)
                     /locus_tag="VFH83_15105"
     CDS             complement(940..2025)
                     /locus_tag="VFH83_15105"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HET6487754.1"
                     /translation="MRIRRMRALGAVLGALLWAGTPGAGSLSAQDQAPAQDLRTGQGQ
                     KDAQDPDGILDSLVRVMATVPEGASTADSLGTVREGSGIVIDSTGLILTIGYLVVEAD
                     RIMVQTASGTVAADFVGYDGDTGFGLVRARSASGLKPMPLGESSALEEGDHLVVRSAD
                     GDAAQVQVISRGEFVSSWEYLLDSAVFTAPAFHDFGGAALILEGKLVGVGSVVTAFEV
                     PGAGRLPANMFVPIDLLKPILQAMVRSGRSGLPAHPWLGVNTAETQQRVVVDSVTPGG
                     PADKAGLKPGDIILDVGRTPVSGIADFYRRVWSVGAAGVSVNLRVLQGDAIRTLTVKS
                     VDRSERLKLFPDMPGSAPAGPAVWLRP"
     gene            2506..3777
                     /locus_tag="VFH83_15110"
     CDS             2506..3777
                     /locus_tag="VFH83_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6487755.1"
                     /translation="MRRFLVLLLIVTVAGFSAFAQNQPTKLTLWTFQELHLAFYSAAV
                     KAWNTQNPGKQIDLDGQVFPYADMHNKLLVAVQSGVGAPDIADIEISKFPNFLQGNIG
                     LVPLNDVIDPFRDKFVQARLAIYAKDGKNYGLPFHVGASVMYYNMDIMKAAGVNIDKI
                     ESWDDYVKAGQQVVAKTGKPMVTVETTDQWSYWPLIAEQKSDFFDPKGKVWLDNPTNV
                     KTLQFLQDLVYKYKIAVTAPGGFHHSEQYYGAMNSGSFGSIWMPMWYMGRFTDYMPDL
                     AGKIAIRPMPRWTKGGFRSAGMGGTGTAVLTQSQHIDLAKKFLFFAKGSKQGNIMIWQ
                     VLGFDPPRWDVWADPAMSAPNKFTEYFQNGDGAFKMLLAVKDEIYPVTLTAQTPDLTD
                     MVRNNIMVQVIQDRSKTPEQALKEAADQLRK"
     gene            3847..4746
                     /locus_tag="VFH83_15115"
     CDS             3847..4746
                     /locus_tag="VFH83_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6487756.1"
                     /translation="MRRSIGSARFSPYLFVLPFIVTFLVFYLYPLVSTVIMSFQEIVP
                     GQTHWIGTLNYERLLNPFMYTAIWNSFRYTVFTILILIPIPVLLAVMLNRPGNPANVV
                     FRGAMFLPTLVSVVVAGTIIRLLFAGSPAAIVNTILHPLGIKPTDWLLGGSASAMTLL
                     VGMATWRWTGVNIVYFMSGLQSIPRDLYEAADIDGASAWYKLFRVTVPLLKPTIIFVT
                     TISVFGGFAMFEESYILWAGQSGGGSPNNVGLTMVGYIYLKGFQGGALGLGSAIGMIL
                     LAIVLGVSFLVLNLFGFFKRDEA"
     gene            4743..>4976
                     /locus_tag="VFH83_15120"
     CDS             4743..>4976
                     /locus_tag="VFH83_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487757.1"
                     /translation="MRSLRTAESRVNALFFLIFLVLCLAFLFPIYSLLLASFRPGRDL
                     MRFGITVKTLAPGDLSLKYLASLLTGRAGAYTVW"
BASE COUNT          898 a         1652 c         1564 g          862 t
ORIGIN      
        1 cgctggcggc gcttgaccat ggagccatgc acgtgcaggg caccatcaac ggatggggag
       61 agcggtgcgg gaacctcaac ctgtgcacgc tgatccccac gctgtgcctc aagcgccagg
      121 actcggccgg cgtgtgcgtc aacctgaagc ttctcacggc cctgtcccgg ttcgtggcgg
      181 agaaggccaa catcatcccg gacaagcgct ggccctacgt cggggagacg gcgttcagcc
      241 acaaggccgg ccagcacgcg gacgtcgtga gcaaggccgc gcggctcatg gagcacgtcg
      301 acggggcgct ggtgggcaac gagcggcgca tcctcctctc ggagctggcg ggcaagtcca
      361 ccatcgtgag gaagatggag cgttacggcg atttcgcgaa gtcggcggag gtggtctccg
      421 ggctgctgga gcagctcaag ctcctcgagg agaagggata cgagtacgag gccgccgagg
      481 gctcgttcga cctgctgatc cgccgggcgc tgggccgcta ccggccgctc ctggagctca
      541 gcaactacca cctggagaac tacaaggcag gcgccacgcc ggcgaagacg gtgggacggc
      601 tgttcgtgcg ggtgaacggc gacaccctga tgggtgcggc cgtcggcgac ggtccggtgg
      661 acacgctcaa cggggcgctc cgggacgccc tgctgccgct gcacccgttc ctgcagaagc
      721 tgaagctcac ggactaccgg gtgcgggtcc tcaaccccga gcgggccacc gcggcgaaag
      781 tgcgggtgtt catcacgacc tcggacggag accagacctg ggacacggta ggcgtggacg
      841 agaacatcat tgaggcctcg tggcaggcgc tcatcgacgg ggtggactac tacttcaaca
      901 acttcgtgct ggaggagaag accggctgac gcctccgcct cagggacgca gccagacggc
      961 cggaccggca ggtgcgcttc ccggcatgtc cgggaacagc ttcagccgct cgctgcggtc
     1021 cacggacttc accgtgagcg tccgtatggc gtctccctgc aggacgcgca ggttgacgct
     1081 gacccccgcg gcgccaacgg accacacgcg gcggtagaaa tcggcgatcc ccgagaccgg
     1141 cgtccggccc acgtccagga tgatgtcccc gggcttcagc cccgccttgt ccgcggggcc
     1201 gcccggcgtg accgagtcca ctaccactct ctgctgggtc tccgcggtgt tgacgccgag
     1261 ccacgggtgg gcggggagcc ccgatcgacc cgagcggacc atggcctgga gtatgggctt
     1321 caggaggtcg atcgggacga acatgttcgc gggaagcctc ccggcgcccg gtacctcgaa
     1381 cgccgtgacc acggaaccca ccccgaccag ttttccttcg aggatcaggg ccgcgccgcc
     1441 gaagtcatgg aacgcgggcg ccgtgaacac ggcgctgtcc agcaggtatt cccacgaact
     1501 gacgaactct ccgcgggaga tgacctggac ctgggcagcg tcaccgtcgg cacttcggac
     1561 caccaggtga tcgccttcct cgagagccga cgactccccg agcggcatcg gtttcagacc
     1621 ggaagcggaa cgcgcacgta cgaggccgaa cccggtatcc ccatcgtagc cgacgaaatc
     1681 cgcggcaacg gtcccggagg cggtctgcac catgatgcgg tccgcttcca ccacgaggta
     1741 gccaatggtg aggatcaacc cggtggagtc gatgacgatt ccgcttcctt cccgcaccgt
     1801 gccaagggaa tccgcggtgg aggcgccttc ggggaccgtg gccatcaccc gcacgaggga
     1861 gtcgaggatg ccgtcggggt cctgggcgtc cttctgcccc tggcccgttc gcaggtcctg
     1921 cgccggcgcc tggtcctgcg cggacaggct cccggccccg ggtgtaccgg cccagaggag
     1981 cgccccaagc accgcaccga gggcccgcat tcgccggatg cgcatcgtac acctccaggg
     2041 cctctcgtcc gactccgctg gatcgggtaa cgcgaggctc catttcaaag atactgatgg
     2101 acttgtgggg aggcataccc tggccgcgcg gcgcaggtcg cccccaccgc gcgccactgg
     2161 ccgagccgac cttgaggctg gatccggagt tcgacctcgt atctatgcac agactggaga
     2221 tttagccttc aaatggcgcc attaatattg gtccgtatca aattgttaga tatccacata
     2281 acggatattt aatgccgtta accgcgaaag ttttatcgaa acagatgctg atcgagccca
     2341 agtgcctatt ttccacagtt gtctatcatt tttcttgaca tatatcaggg atgaccttag
     2401 aatccgcgct cgtgggggcg ttgcccctgg cgccaggcgc agccgcccgc gcctcccaag
     2461 tcaagccggg ctgttcgaac aggacagcgc gagacggagc caagcatgcg cagattcctc
     2521 gtattgcttc tcatcgtcac ggtggccggg ttcagcgcgt ttgctcagaa ccagccgacc
     2581 aagctcaccc tctggacgtt ccaggagctt cacctggcgt tctacagcgc cgcggtgaag
     2641 gcgtggaaca cccagaaccc gggcaagcag atcgacctcg acgggcaggt gttcccgtac
     2701 gccgatatgc acaacaagct cctggtggcg gtgcagtcgg gcgtgggagc ccccgacatc
     2761 gcggacatcg agatcagcaa gttccccaat ttcctgcagg gcaacatcgg cctcgtgccg
     2821 ctgaacgacg tgatcgatcc cttccgcgac aagttcgtcc aggcccgcct ggcgatctac
     2881 gccaaggacg ggaagaacta cggcctgccg tttcacgtgg gcgcgtcggt catgtactac
     2941 aacatggaca tcatgaaggc cgccggcgtc aacattgaca agatcgaatc ctgggacgac
     3001 tacgtgaagg ccggccagca ggtggtggcc aagaccggca agccaatggt gacggtggaa
     3061 accacggacc agtggtcgta ctggcccctc atcgcggagc agaagtcaga cttcttcgat
     3121 cccaagggga aggtctggct cgacaacccg accaacgtca agacgctgca gttcctccag
     3181 gacctggtgt acaagtacaa gatcgcggtc accgcgccgg gcgggttcca ccattccgag
     3241 cagtactacg gggcaatgaa ctcaggcagc ttcggttcca tctggatgcc catgtggtac
     3301 atgggccgct tcaccgacta catgccggac ctcgccggca agatcgccat ccggccgatg
     3361 ccccgctgga ccaagggcgg cttccgctcc gccggcatgg gcgggacggg caccgcggtc
     3421 ctcacgcagt cccagcacat cgaccttgcc aagaagttcc tgttcttcgc caagggctcg
     3481 aagcagggca acatcatgat ctggcaggtc ctcgggttcg atcctccccg gtgggacgtg
     3541 tgggctgatc ctgccatgag cgcaccgaac aagttcacgg aatacttcca gaacggggac
     3601 ggcgcgttca agatgctgct cgcggtcaag gacgagatct acccggttac gctcacggcc
     3661 cagacaccgg acctgacgga catggtccgc aacaacatca tggtccaggt catccaggac
     3721 cgaagcaaga cgccggaaca ggcgctgaag gaagcagccg accagctccg caagtaggcg
     3781 gctggtcctt cgcatggagc ccggctcctg ccgggctccc cgttactcca ccggcacggg
     3841 ggcatcgtga gaagaagcat cggctccgcc aggttctcgc cgtacctgtt cgtcctcccc
     3901 ttcatcgtca cgttcctcgt gttctacctc taccccctgg tgagcaccgt gatcatgagc
     3961 ttccaggaaa ttgtgcccgg gcagacgcac tggatcggga cgctcaacta cgagcgcctg
     4021 ctgaacccgt tcatgtacac ggcgatctgg aacagcttcc gctacaccgt gttcactatc
     4081 ctgatcctca tccccatccc ggtgctgctc gccgtcatgc tcaaccggcc gggcaacccg
     4141 gccaacgtgg tattccgcgg cgcgatgttc cttcccacgc tggtgtcggt ggtcgtggcc
     4201 ggcacgatca tccgcctcct gtttgccggg agccccgccg ccatcgtcaa cacgatcctg
     4261 caccccctcg ggatcaagcc cacggactgg ctgctcggcg gttcggcctc cgccatgacg
     4321 ctgctggtgg gcatggccac gtggcggtgg accggggtga acatcgtgta cttcatgtcg
     4381 ggcctgcaga gcatcccgcg ggacctctac gaggcggcgg acatcgacgg cgcctccgcc
     4441 tggtacaagc tgttccgggt cacggtgccg ctcctgaagc ctaccatcat cttcgtcacc
     4501 accatcagcg tgttcggcgg cttcgcaatg ttcgaggaga gctacatcct gtgggccggc
     4561 cagtccggcg gcggctcccc gaacaacgtc ggcctcacca tggtgggata catctacctc
     4621 aagggcttcc agggaggcgc cctggggctg gggtccgcca tcgggatgat cctgctcgcc
     4681 atcgtgctcg gggtgagctt cctggtgctg aacctgttcg ggttcttcaa gagggacgag
     4741 gcatgaggtc gctgcgcacc gccgaatcca gggtgaacgc cctgttcttc ctcatattcc
     4801 tcgtgctgtg cctggcgttc ctcttcccga tctactcgct gctgcttgcg tctttccggc
     4861 ccggccggga cctcatgcgc ttcgggatca cggtcaagac gctggcgccg ggagacctat
     4921 cgctgaagta cctcgcgagc ctgctcaccg gccgggccgg cgcctacacc gtgtgg
//
LOCUS       DASRZS010000620         5757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1133359, whole genome shotgun sequence.
ACCESSION   DASRZS010000620 DASRZS010000000
VERSION     DASRZS010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133359"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFH83_15125"
     CDS             <1..561
                     /locus_tag="VFH83_15125"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET6487758.1"
                     /translation="PIFIAPLEAQSILIGLGSVKMPRPLTHDLFISVLENLQCSINRV
                     EITSLKEGTYYAKLILNSSGDEIAVDARPSDCLALAVRVKCQIYIDEAVVDEAGISVK
                     MVEEKTKELRTGAAEELATEEPSTEEAPEAAESSEETESLAQVTPAGQESEMAKLRKL
                     LDAAVEEENYEEAARIRDKMRELEST"
     gene            complement(589..1287)
                     /gene="pyrH"
                     /locus_tag="VFH83_15130"
     CDS             complement(589..1287)
                     /gene="pyrH"
                     /locus_tag="VFH83_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET6487759.1"
                     /translation="MSSHIIAISLGGSIIAPRGVDVDFVSGFVRLVEGHLSRNPDARL
                     IVVCGGGGLARDYQAALRGIRPDASSEDQDWVGVAATRVNGELVRRAFGRLCGEDLVI
                     DPTAVSVFAGKVLVAAGWKPGFSTDNDAVVLAQRFSADTVINLSNIAKVYTGDPRTQP
                     SARPVDRLAWKEMLQLVGGPWSPGTNTPFDPTAARAAAASRIRVIVAAGRDLDNLSRI
                     LAGEAFVGTTIGPD"
     gene            1384..2340
                     /locus_tag="VFH83_15135"
     CDS             1384..2340
                     /locus_tag="VFH83_15135"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HET6487760.1"
                     /translation="MASKKVFVADSLAREGIEEFARYPGIELVVKTGLPADKLALEIG
                     DSQGLVVRSATQATRRIIEAAPALKVIGRAGAGVDNIDVPAATARGILVLNTPGGNAE
                     AAAELAIAMMFALARQIAQADASMKTGTWDKKSFSGVELLGKTLGLIGVGNVGGIVGK
                     RALGLGMKVVATDPFVDAARMAAAGFQAASFDEVISGADFLSVHSPKNEQTMGMLNAA
                     AFARMKKGVFLVNCARGGIVVERDLIAALDSGIVRGAGVDVFEKEPPEDWSLARHPKV
                     VATPHLGATTGEAQVNVSVMIARQIGAYLSRGEIVNAVNAPA"
     gene            2474..3262
                     /locus_tag="VFH83_15140"
     CDS             2474..3262
                     /locus_tag="VFH83_15140"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HET6487761.1"
                     /translation="MSADITDILREWEFDPDNQIRIIQADDGRQVLQVRQPLGVEQYE
                     LDGRPDGKKPFGRDTALEEYQARLETFRASHGTEDGFSLDKDDVAALQGEGILFYYRY
                     LVLFQIGDFVRTARDTEHNLTIFSLVERFVDSEEERKGMLQYRPYVVRMNAIARAMIS
                     LHKEMKSAAEDILNNAISMIEGMREIDTPAFQFERVRSLNYLKATLKQIHEKKDSPID
                     ALKTELAAAVEDEDYERAAVLRDRLRDIRAEEPEEEETPTESEE"
     gene            3377..3724
                     /locus_tag="VFH83_15145"
     CDS             3377..3724
                     /locus_tag="VFH83_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6487762.1"
                     /translation="MIKTHAEMEREVRQKMRGGQGSVEIVHIFRPQELKGKVRLMAHL
                     RLEKGSSIGFHVHEGEEEVFYILRGAGMVSEGAEPRRVTAGDAVLTGGGAGHAIENPG
                     DEPLEFMATILTY"
     gene            3731..>5757
                     /locus_tag="VFH83_15150"
     CDS             3731..>5757
                     /locus_tag="VFH83_15150"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6487763.1"
                     /translation="MLSLFRRDAAQHFATSFHWLEERPLPERTERFYSTRYQRGAYSL
                     TLAKDSYFAWETLSAARSYDDFVLEADLEIDPSNGHSAVGVVFRHVTDENFYSFLISS
                     RGNFRFDLLFNNHPAHLVEWTPLAEPEAGRRRLSVIARGPRFTFLVDEEWIAELDDEV
                     LPSGGIGFAAQNFAGSGPGIFRLCRLSLEARPLIVERDHLRWHSFLPVSPASRLRLAE
                     TLCAMGSYEAAAVQLRRALREREGTVRERFLLAECYAHLSLYPEALAELDQVQKREPG
                     HREARIERANLLYLTNQLDKAREAISAAVADPQGTAGPAHWNLLGNVEYALGNWQRAA
                     DAYLRAVELDPKMSLFLSNAARALERAGRAPDATDAYLRAARLLFAEESFDELSTVMP
                     RLTALAPHIAEVRALEAKMLYREGKADEAFRILSELDAHGSRDASVHYLLGIMLNERG
                     RREAALPHLLRAAELEPDFPLNQFRLAETLHLLGEDCGPALERALELAPRDPWTNNLA
                     GMLRMEAGDFGRAAAHLRVAQEAAPEEREIAVNLSEALSLSGDHAQALTVLDDFASRV
                     ADSARVANQRGNILGRLGEHARAVREYETAIRLDPQDWHVKENCAAACLQLDMVHRAE
                     ELLAQIEPEHPSASVYNLLGNVAVLKGETARGEVAYRTGLELDPENRDLSV"
BASE COUNT         1011 a         1898 c         1965 g          883 t
ORIGIN      
        1 cccatcttca tcgcgcccct ggaggcccag tccatcctga tcggtctggg gagcgtcaag
       61 atgccgcgtc ccttgaccca tgacctcttc atcagcgtgc tggagaacct ccagtgctcc
      121 atcaatcgcg tggagatcac ctccctgaag gaggggacct actacgcgaa gttgatcctg
      181 aactcctccg gggacgagat cgccgtggac gcccgaccgt cggactgcct ggcactggcg
      241 gtccgggtga agtgccagat ctatatcgac gaggccgtgg tggacgaggc cggcatctcc
      301 gtgaaaatgg tggaagagaa aaccaaggag ctgcggaccg gcgccgccga agagctggcc
      361 accgaggagc cgtccacgga agaagccccg gaggccgcgg aatcctcaga ggagaccgag
      421 tccctggccc aggtgacgcc ggccgggcaa gagtccgaga tggcgaagct ccgcaagctc
      481 ctggatgccg ccgtagagga agagaactac gaggaagcgg cccgcatccg ggacaagatg
      541 cgggagttgg agagcacgtg atcggcgccg gcaggcgccg gtagcccttc agtcggggcc
      601 gatcgttgtc ccgacaaacg cctctccggc gaggatgcgg gagaggttat cgagatccct
      661 gccggcagcg acgatgaccc ggatcctgga cgctgccgcg gcccgcgccg cggtcgggtc
      721 gaagggcgtg ttcgtgcccg ggctccaggg ccctcccacc agctggagca tctctttcca
      781 ggcgagccgg tccaccggcc gggcgctggg ctgcgttcgg ggatcccccg tgtatacctt
      841 cgcgatattc gagagattga tgacggtgtc cgcggagaag cgttgagcca ggaccaccgc
      901 gtcgttgtcc gtcgagaagc ctggcttcca gcccgcggcc accaggacct tcccggcgaa
      961 aaccgaaacc gccgtagggt cgatcaccag gtcctctccg catagcctgc cgaaagctcg
     1021 tcgcaccagc tctccgttca cccgggttgc cgccacgccc acccagtcct ggtcctcact
     1081 ggaagcgtcc ggtcggatcc ctcgaagggc cgcctggtag tctcgcgcca gcccccctcc
     1141 gccgcacacc acgatgaggc gcgcgtccgg gtttcggctc aggtgcccct ccaccagccg
     1201 tacgaaaccg gagacgaagt cgacgtccac cccgcggggg gcgatgatcg acccgcccag
     1261 ggagatcgcg atgatgtgtg aggacatggg tctactatag aggaatccgg gcgtcagagg
     1321 aatcgggacg aggcggttca tgcgttgctt gcaaaaacat gcatcacggt ttacaacgtg
     1381 cgcatggcaa gcaaaaaggt gttcgtagcg gactccctgg cccgggaagg gatcgaggag
     1441 tttgcccgct atcccggcat cgagctggtg gtgaagaccg gtctgcccgc ggacaagctc
     1501 gctctggaga tcggtgactc ccagggactg gtggtgcggt cggccaccca ggccacgcgt
     1561 cggatcatcg aggcggctcc cgccctcaag gtcatcggcc gagccggcgc cggagtggac
     1621 aacatcgacg tccccgcggc aacggctcgt ggcatcctgg tcctcaatac tccgggcggc
     1681 aacgcggagg cggcggccga gctcgccatc gccatgatgt tcgccctggc ccggcagatc
     1741 gcccaggcgg atgcctcgat gaagaccggc acctgggaca agaagagctt cagcggcgtg
     1801 gagctcctcg gcaagacgct cgggctcatc ggggttggca acgtgggcgg catcgtgggc
     1861 aagcgggccc tgggtctggg gatgaaggtc gttgccacgg acccgttcgt ggacgcggcc
     1921 aggatggcgg ccgcagggtt ccaggcggcg agcttcgacg aggtgatcag cggggcggac
     1981 ttcctctccg tccattcccc caagaacgaa cagaccatgg ggatgctcaa cgcggcggcc
     2041 tttgcgcgga tgaagaaggg cgtgttcctg gtcaattgcg ccaggggcgg gatcgtggtc
     2101 gaacgggacc tgatcgccgc cctggacagc ggcatcgtcc gcggcgccgg cgtcgacgtc
     2161 ttcgagaagg agcctccgga ggactggagc ctggctcgtc acccgaaggt ggtggccacc
     2221 cctcacctgg gcgccacgac gggggaggcg caggtgaacg tttcggtcat gatcgcgcgc
     2281 cagatcggcg cctacctgtc ccgcggggag atcgtcaacg cagtgaacgc ccccgcctga
     2341 ggtgccggcc gcgggcgcca gcttggcaac ctgggtctag atgggcggcg aagcgttgcg
     2401 ccgctcgggt ccgccgttgc cgaagcttga cacagggggt acattggtgg cgtgccgctg
     2461 agggggcggg aacgtgagcg cagacataac cgacattctc cgggaatggg agtttgaccc
     2521 ggacaaccag atccgcatca tacaggcaga cgacggacgt caggtgctgc aggttcgtca
     2581 gccgctgggc gtggaacagt acgagctgga tggtcggccg gacggcaaga agccctttgg
     2641 gagagacacg gcgctggagg agtaccaggc ccgtctggaa acgttccgcg cgtcccacgg
     2701 cacggaagac gggttcagcc tggacaagga tgacgtagcg gcgctccagg gcgaagggat
     2761 cctcttctac tatcgctatc tggtcctctt ccagatcggc gacttcgttc ggacggcccg
     2821 ggataccgag cacaacctga ccatcttctc tcttgtcgag cggttcgtgg atagcgagga
     2881 ggagcgcaag ggcatgctcc agtatcggcc ctacgtggtg cgcatgaacg ccatcgccag
     2941 ggcaatgatc tcgctgcaca aagagatgaa gagcgcggcg gaagacatcc tgaacaacgc
     3001 catctccatg atcgaaggca tgcgggagat cgacactccg gcgttccagt tcgagcgggt
     3061 ccgatcgctc aactacctca aggcgacgct caagcagatc cacgagaaga aggactcccc
     3121 gatcgatgcg ctgaagacgg agctggccgc cgccgtggag gacgaggact acgagcgggc
     3181 cgccgttctc cgagaccgcc tccgcgacat ccgtgccgaa gagcccgagg aagaggagac
     3241 tcccaccgag tccgaggagt agggggccgt cccccggccg gctcgccgag gaaccgccgg
     3301 cccactgggt cgggccacca cgggcgcctt gctgtatcct ccgctccctg ataccctgcg
     3361 gagcgggagc caaccgatga tcaagacaca cgccgagatg gagcgagagg tgcgccagaa
     3421 gatgcgcggc ggccagggct ccgtggagat cgtgcatatc ttccgacccc aggagctcaa
     3481 ggggaaggta cgtctgatgg cgcacctgcg cctggagaag ggctcctcca tcgggttcca
     3541 cgtccatgag ggagaggaag aagtcttcta tatcctgaga ggagccggca tggtgagcga
     3601 aggggccgag ccccgccgcg tgaccgccgg ggacgccgta ctcaccggcg gaggcgccgg
     3661 gcacgccatc gagaacccgg gggacgaacc gctggagttc atggccacca tcctgacgta
     3721 ctgacaccgc atgctctcgc tattccggcg ggacgccgcc cagcacttcg ccacctcgtt
     3781 ccactggctt gaggagcgtc cgctccccga gcgcacggag aggttctaca gcacccgcta
     3841 ccagcgcggg gcatactcgc tcacgcttgc caaagacagc tacttcgcct gggagacgct
     3901 ctccgcggcg cgctcctatg acgacttcgt gttggaggcc gacctcgaga tcgatccctc
     3961 caacggccac tcggccgtgg gggtcgtgtt ccgccacgta acggacgaga acttctactc
     4021 cttcctgatc tcgtcccgag gcaacttccg cttcgacctc ctcttcaaca accatccggc
     4081 gcacctggtg gagtggacgc cgctggcgga gcccgaggcc ggccggcgcc ggctgtccgt
     4141 gatcgcccgc ggtccccggt tcaccttcct ggtggacgag gagtggatcg ccgagctgga
     4201 tgacgaagtg ctgccctccg gcgggatcgg cttcgcggct cagaacttcg ccggctcggg
     4261 gcccggcatc ttccgcctct gcaggctgtc cctggaggcg cggccgctca tcgtggagag
     4321 agaccacctc cgctggcatt cgtttctccc cgtgtccccg gcatcgcggc tgcgcctggc
     4381 ggagaccctt tgcgccatgg gcagctacga ggccgcggcg gtgcagctcc gacgggccct
     4441 gcgggagcgg gagggcaccg tgcgggagcg cttcctgctg gcggagtgct acgctcacct
     4501 gtccctctat ccggaggcgc tcgcggagct ggaccaggtg cagaagcgcg agccgggcca
     4561 tcgggaagcg cgcatcgagc gcgccaacct cctttacctc accaaccagc ttgacaaggc
     4621 gcgagaggcg atctcggccg ccgtggcgga tccgcagggg acggccggcc cggcccactg
     4681 gaacctcctg ggaaacgtcg agtacgcgct gggcaactgg cagagggcgg cggacgccta
     4741 ccttcgcgcc gtggagctgg atccgaagat gtcgctcttc ctgagcaacg ccgcgcgagc
     4801 cctggagcgg gccggacgcg cgccggatgc gacggacgcc taccttcgag cggcgcggct
     4861 cctctttgcc gaggagtcgt tcgacgagct gtccaccgtg atgccccgac tcacggcgct
     4921 tgccccgcac atcgccgagg tccgcgccct ggaagccaag atgctctatc gcgagggaaa
     4981 ggcggatgag gctttccgga tcctgagcga gctggatgcc cacgggagtc gggacgcctc
     5041 ggtgcactac ctcctgggga tcatgctcaa cgagcggggc cgtcgggagg cggcccttcc
     5101 gcacctgctg cgagccgccg agctcgagcc ggacttcccg ctcaaccagt tccgcctggc
     5161 agagacgctc cacctcctgg gagaggattg cggaccggct ctggagcgcg ccctggagct
     5221 tgccccgcgg gacccctgga ccaacaacct ggccggcatg ctccggatgg aggccgggga
     5281 cttcggccgc gcggcggccc atctgcgggt tgcccaggag gcggcgccgg aggaacggga
     5341 gatcgccgtc aacctctccg aggcgctgtc gctctcgggg gatcacgccc aggccctcac
     5401 ggtcctcgac gactttgcga gccgcgtcgc cgacagcgcc cgcgtagcca atcagcgcgg
     5461 gaacatcctg ggtcgactgg gggagcacgc gcgcgcggtc cgcgagtacg agacggccat
     5521 ccgtctggac ccccaggact ggcacgtcaa ggagaactgc gccgccgcct gtctccagct
     5581 cgacatggtc catcgcgccg aggagctcct ggcccagatc gagccggagc acccgtcggc
     5641 ttcggtctat aacctgctgg ggaacgtggc cgtgctcaag ggcgaaaccg cccggggaga
     5701 ggtggcctac cgaaccggtc tggagctgga tcccgagaac cgggacctct ccgtgaa
//
LOCUS       DASRZS010000621         3225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5356573, whole genome shotgun sequence.
ACCESSION   DASRZS010000621 DASRZS010000000
VERSION     DASRZS010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5356573"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFH83_15155"
     CDS             complement(<1..279)
                     /locus_tag="VFH83_15155"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018302837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6487764.1"
                     /translation="MKRFLWGVPAVLLIAVLSGCSPTVVEKKVVDVQYKGTLADGSTF
                     SESQPSHPLEFLVGAHQIIPGLEKAVLGMKAGQKKKIEIKAADAYGAYD"
     gene            433..2877
                     /locus_tag="VFH83_15160"
     CDS             433..2877
                     /locus_tag="VFH83_15160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6487765.1"
                     /translation="MAVLAQVRSILHRLRDGALYLPNPTFANPAWEDARPRVLILRLS
                     CFRDVARSSTHVLLAREIRASLPHAWIDMAFLPLRSDGLEMEAAGLPLILGTQSLRPL
                     RDFDLVLVSNSYLLEQVNLPFLLRRSGVPVWSSQRDEGWPILVLGGSNASAAHGLVSD
                     SGDSVVDAIFFGEGEGKVGALAQALVGAGAKGERLRRAARAAEGLWVAGPSEGAVRSV
                     CPSEAALAGAHLGPVLPGPEAATARLAITLGCPCLCSFCFEGHDRKPFRQIPLDRIVT
                     AARELKRSTGAITLEVESFNFNTHRELPEILLSLNRLFFRVNLMSQRADILADDPLLL
                     DLEMASDKHSFTLGIEGISERQRAFLHKSLEEPRIRRVLEECASRRIREIKLFYLLTG
                     RETEEDFLEFSGLLKWLKDLQGRAVSRPRILFSFGMLVRMPFTPLRHDPAVLDERLWK
                     GIAGRAKSLCQTNGFEFRLALPWSTYAATQALASADSAGASTLVQAIAGHGPLIDDSL
                     DEEEERTVREWLTRHADELGSGKPLGFPFPFPFLDTEREQDRLYRQYEDARAGRDRGY
                     HGRDVAERGSLRPSIEALQGLIHAKRRLQPLRVVARVPREAAGMGREWTDAWLCRRLL
                     ELHPELLDNVLCVRESLIASLPSFGERTPWFGESIAEVMAWDPEALARVLGAAVGGFH
                     ELTEAEALEKLRLRVSLPASGAKDAPSGLAAFLRDHHGPVTLRRKGDSVELMASAKAI
                     QKRLLLAGTAWRTTAASNALEEAGDLWVLELVIGTRFPLAEWLAVCCGPGADRTALVE
                     VLSISPWDRRPGASSR"
     gene            complement(2837..>3225)
                     /gene="trxA"
                     /locus_tag="VFH83_15165"
     CDS             complement(2837..>3225)
                     /gene="trxA"
                     /locus_tag="VFH83_15165"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET6487766.1"
                     /translation="SVSWELPEGGESVINGGGGPLPSSFLELVRVSDLPVLADFWAEW
                     CGPCRLVTPAIERLAREYSGRLLTVKVNVDRKPDVAQAYEVDGIPTIVLFWKGAPLMR
                     LTGAYPYEAIRQSLIESWPRDAGPTG"
BASE COUNT          492 a         1100 c         1067 g          566 t
ORIGIN      
        1 atcgtatgcg ccgtaggcgt cggcggcctt gatctcgatc ttcttctttt gcccggcctt
       61 catgcccagc acggcctttt ccagcccggg gatgatctgg tgcgccccca ccaggaactc
      121 caggggatgg cttggctggg actcgctgaa cgtgcttccg tcggcgagcg tccctttgta
      181 ctggacgtct acgaccttct tctccaccac ggtcgggctg caaccggaga ggaccgcaat
      241 gagcagaacg gccggcacac cccagagaaa cctcttcacg atggactcct ttggcgccga
      301 gcgcgaactg cggccgagcg aggatcggtg acgcccaagc gctaatcacg gccgagcgca
      361 gctaagatgc cgccgaacgc ggatcggcta ggataggcaa aagagggcgt cccgtctaca
      421 ctgatggagc ccatggccgt ccttgcccag gtacgatcga tccttcaccg gctgcgggac
      481 ggtgccctct atctccccaa cccgacgttc gccaaccccg cctgggagga tgcgcgcccc
      541 cgtgtgctca tcctgcgcct gtcttgcttc cgcgacgtgg cgcggtcctc cacgcacgtc
      601 ctcctggccc gcgagatccg cgcgtccctt ccgcacgcct ggatcgacat ggcttttctg
      661 ccgctccggt cggatggcct cgagatggag gcggccggcc ttcccctgat cctgggcacc
      721 cagtcgcttc ggccgcttcg ggacttcgac ctcgtgctgg tctccaactc gtacctcctg
      781 gaacaggtga accttccgtt cctgctccgc cgctcgggtg tgccggtgtg gtcgagccaa
      841 cgggacgagg gctggcccat tctggtcctg ggcggatcga acgcgagcgc cgctcacggc
      901 ctggtctccg actccggcga ttccgtggtc gacgcgatct tcttcggcga aggggagggg
      961 aaggtcggag cgctggccca ggcgctggtc ggggccggcg caaagggtga acgcctcagg
     1021 cgcgcggcgc gtgccgcgga aggtctctgg gtcgcaggac cctcggaggg cgcggtccgg
     1081 tccgtctgtc cgtcggaggc cgcgctggcg ggtgcgcacc tgggtccggt gctgccgggt
     1141 cccgaggccg caacagcgcg gctggccatt accctgggtt gcccgtgtct ctgttccttc
     1201 tgcttcgagg gacacgaccg aaagccgttc cgccagatcc ccctggaccg gatcgtcacg
     1261 gccgcacgag agctcaagcg ctccaccggc gccatcacgc tggaggtgga gagcttcaac
     1321 ttcaacacgc accgcgagct gcccgagatc ctgctctccc tcaaccgtct gttctttcgg
     1381 gtgaacctga tgagccagcg cgcggacatc ctggccgacg atcccctgct tctggacctc
     1441 gagatggcct ccgacaagca cagcttcacc ctggggatcg aagggatcag cgagcgtcag
     1501 cgcgcgttcc tgcacaagag cctggaggag ccgcgcatcc gcagggtgct ggaggaatgc
     1561 gcatcccgcc gcatccgtga gatcaagctc ttctacctct tgaccggacg cgagacggag
     1621 gaggatttcc tcgagttctc cggcctcctg aagtggctca aagacctgca gggccgcgcg
     1681 gtctccagac cccggatcct cttcagtttc gggatgctgg ttcgcatgcc ctttaccccg
     1741 cttcgccacg atccagccgt gctcgacgag aggctctgga agggcatcgc gggcagggcc
     1801 aagtctctct gccagaccaa cggcttcgag tttcgcctgg cgctcccctg gtcaacgtat
     1861 gccgcaaccc aggcactggc ttcagcggat tcggccggcg cctctaccct ggtccaggcg
     1921 attgcgggac acgggcccct gatcgacgat tcgctcgacg aagaggagga gcggacggtc
     1981 cgggagtggc tcacccgtca cgcggatgag ctcggatcgg gcaagcccct tggattcccc
     2041 ttccccttcc cgttcctgga cacggagcga gagcaagacc gcctctaccg ccagtacgag
     2101 gatgcccgcg ccggccggga tcgcggctat cacggccgcg acgtcgcgga acgcggatcc
     2161 ctccgcccgt ccatcgaggc gctgcaaggt ctgatccacg ccaagcggcg gctccaaccc
     2221 cttcgggtgg tggcgcgggt gccacgggag gcggccggca tggggcgcga gtggacggac
     2281 gcgtggctgt gccggcggtt gctcgagctc caccccgaac tgctggacaa cgttctgtgc
     2341 gtccgcgagt cgctcatcgc ctccctgccc tctttcgggg agcgcacccc gtggtttggg
     2401 gagagcatcg cggaggtcat ggcttgggac ccggaggcac tggcccgcgt gctcggggct
     2461 gccgtcgggg gcttccacga gctgacggag gccgaggccc tggagaagct ccggcttcgc
     2521 gtctcactgc cggcatcggg ggccaaggac gccccctcgg ggttggccgc gtttctgcgg
     2581 gatcaccacg ggcccgttac gcttcgccga aagggcgatt ccgttgagct gatggcatcc
     2641 gcaaaggcga tccagaagag gctgcttctg gcgggaacgg cgtggaggac cacggccgca
     2701 agcaacgccc tggaggaagc gggcgatctc tgggtcctgg agttggtgat cggaacgcgg
     2761 ttcccgctgg cggagtggct cgccgtctgc tgcggcccgg gcgcggaccg tactgcgctg
     2821 gtggaggtcc tgtcgatcag cccgtgggac cggcgtcccg gggccagctc tcgataagac
     2881 tctggcggat ggcctcgtag gggtacgcgc ccgtgaggcg catcagcgga gcgcctttcc
     2941 agaacagcac gatggtcgga atgccgtcca cctcatatgc ctgggccacg tctggcttgc
     3001 ggtccacgtt cactttcacc gtcaggagtc tgccggagta ttcacgggca aggcgctcga
     3061 tggcgggtgt gaccagccgg cagggcccgc accactcggc ccagaagtcc gccagtacgg
     3121 gcagatcgct cacgcgaacc agctcgagaa acgagctggg aagcggcccg ccgccgccgt
     3181 tgatcaccga ctcacctccc tccggcaatt cccaagatac tgatc
//
LOCUS       DASRZS010000622         4046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4965619, whole genome shotgun sequence.
ACCESSION   DASRZS010000622 DASRZS010000000
VERSION     DASRZS010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4965619"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFH83_15170"
     CDS             complement(<1..228)
                     /locus_tag="VFH83_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="octanoyltransferase"
                     /protein_id="HET6487767.1"
                     /translation="MSWRLILHPRMEGRSDMAIDEALLEAVGNGRSGPVVRLYGFSPA
                     TLSLGRFQRIQDRLIHDRLTADGVTLVRRPTG"
     gene            complement(228..1166)
                     /gene="trxB"
                     /locus_tag="VFH83_15175"
     CDS             complement(228..1166)
                     /gene="trxB"
                     /locus_tag="VFH83_15175"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET6487768.1"
                     /translation="MKTDQDLIIVGGGGAGLSAAQYGARGNLRTLLLEEFSSGGQALL
                     INELENYPGFPDPVSGFDFSQKMEDQAKRFGATIDNATVSSIRREDDHFVLETDKGKL
                     TSLAVILATGAKHRELGIPGETEFAGKGVSYCATCDGPFFKGKRMVVVGGGDAACDEA
                     MYLANLAQSVLMIHRRDKFRAQKSLASRTLANPKIQVRFNTELRRISGDKVVKTVHLF
                     DNQANREYTEEVSAVFIFIGSDPKSGLVKDLGVRLDDVGYVETNHKMETGIPGLYAVG
                     DVRASPFRQLVVAAGEGAVAAHVAGQYIDEVRGEAY"
     gene            1253..1756
                     /locus_tag="VFH83_15180"
     CDS             1253..1756
                     /locus_tag="VFH83_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487769.1"
                     /translation="MDTDTRALTRTLTDHMEAEAAIFAGLGLEMTRLREVFQAKHWTP
                     ALQIAAEIERSAVRVEAADNARDEAFVRLRDHLGLPHETAFSAVLPSLPPGERRELED
                     SWRRLRLAVVRLRTATGRMRYSSEALADALNRILEGIFPYRRGKIYSRHGTANAVSGA
                     LLVDQRR"
     gene            1768..3648
                     /gene="flgK"
                     /locus_tag="VFH83_15185"
     CDS             1768..3648
                     /gene="flgK"
                     /locus_tag="VFH83_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HET6487770.1"
                     /translation="MNSTFAPIELGKRGLVATTQGIQTVGHNMSNAAVEGYSRQRVEL
                     TASPPLDEPQLNREDRPGQLGQGVETARIVRLKDMLLEGRIVSEQNVEGYWDARDKYV
                     LMMEQIYNEPTEHSVRTLMDKFWDSWQELSLNPTEMASRSAVLQRGKALLDGVQSHYQ
                     QLHSVRDMIEGDVQGVVTQVNEITKSIAGLNGEIVRAQALGDNPNDLLDRRDLLVGKL
                     STLIDISVRYNDPAGEFVVTTGGTHIVQGQHSETIALSANKSNEGYSDLVWEDTRQAA
                     IVRGGKLASLIELRDGDARSEIQSLDQLAMHFSDLVNEIHRQGYGLNGDTGKDFFVQR
                     PFVTNVTGAYDSNGDGALDSTYLFRVTGANALQPKEQIGLAGTLTLPGHDGDVKIDYH
                     PTDTVDDLITRINLSGSEVVARLNSEGKLSLKAAPAANTGYPDFVIRGLEDSGPFLVG
                     YAGVLKASGPAGAYSWNRPDAVTTLAGPDTQYAVAPLAHPAGWMEINPLLAQDPGSIA
                     AAAGVDGRPEGVDNGAAALAIAQLRTTPVMVGASSTFDGFLADRVASIGLKGEEAKQS
                     LDTSRLVMKNLTDTRESISGVNVDEELTQMITYQNGYAAIARFVTTVDSLLDIIINRM
                     GV"
     gene            3662..>4046
                     /locus_tag="VFH83_15190"
     CDS             3662..>4046
                     /locus_tag="VFH83_15190"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487771.1"
                     /translation="MKRISSQLPASDSSFYLRQREVEMNDMSNKIGAQSTIKDLRDDP
                     LGAAKSVRLQSEIFRLDRYGKNIQDIRARFTTAEGNLQSAMDILQRVRELAVQGANGT
                     LDTQQMGYIGDEVDQLLGELLSIGNS"
BASE COUNT          736 a         1351 c         1336 g          623 t
ORIGIN      
        1 tcccgtggga cgccggacca gggtaacccc gtccgcggtc agccggtcgt ggatcagccg
       61 atcctggatc cgctgaaacc gccccaggga aagggtagcc ggcgagaaac cgtacagccg
      121 gaccacgggg ccgctgcggc cattccccac agcctccagg agcgcctcgt cgatggccat
      181 gtccgaccga ccctccatgc gggggtggag gatcaaccgc cagctcacta gtaggcttcc
      241 cctcggacct cgtcgatgta ctgcccggcg acatgggcgg ccacggcgcc ctccccggcc
      301 gccaccacga gctggcggaa gggggaagcg cgtacgtctc cgaccgcgta gaggcccggg
      361 attcccgtct ccatcttgtg gttggtctcg acgtagccca cgtcgtccag gcggacgcca
      421 aggtccttga ccagcccgct cttggggtcc gacccgatga agatgaagac cgcggagacc
      481 tcctccgtgt actcgcggtt ggcctggttg tcgaagagat ggacggtctt caccaccttg
      541 tcccccgaga tccggcgcag ctccgtgttg aaccggacct ggatcttggg gttggcgagg
      601 gtcctggacg ccagagactt ctgggcacgg aacttgtccc gtcgatggat catcaagacg
      661 gactgcgcca ggttggccag gtacatggcc tcgtcacagg cggcgtctcc cccgcccacc
      721 acgaccatcc gcttcccctt gaagaacggg ccatcacagg tggcgcagta cgagacgcct
      781 ttgccggcga actcggtctc acccgggatg cccagctccc ggtgtttcgc cccggttgcc
      841 aggatgaccg ccagggacgt gagctttccc ttgtccgtct ccaggacgaa gtgatcgtcc
      901 tcccgacgga tggagctgac ggtcgcgttg tcgatcgtgg ctccgaatcg cttcgcctgg
      961 tcctccatct tctggctgaa gtcgaacccg ctcacggggt ccgggaaccc ggggtagttt
     1021 tccagttcgt tgatcaagag cgcctgtccc cccgaggaga actcctccag gaggagggta
     1081 cgcaggttgc cgcgtgctcc gtactgggcc gcggagagcc cggcaccgcc cccgccgacg
     1141 ataatgagat cctgatccgt tttcatggtg aatcctctcg gtggaagata ccagattggc
     1201 ccgctcatgg gaagaaaaga actaccgaca ctgtagaaag aggagcagac ccatggacac
     1261 cgacacgcgc gcgctcacgc gcaccctcac cgaccacatg gaggccgagg ccgcgatctt
     1321 cgccgggctc ggcctggaga tgacccggct tcgagaggtc tttcaggcaa agcactggac
     1381 gcccgcgctc cagatcgccg ccgagatcga gcggtccgcc gttcgggtcg aagcggccga
     1441 caacgcccgg gacgaggcct tcgtccggct tcgggaccac ctggggctcc cgcacgagac
     1501 cgcgttctcc gcggtgcttc cgtccctgcc tcccggggag cgcagggagc tggaggatag
     1561 ctggcgccgt ctgcgcctgg cggtggtccg tctgcggacc gccacgggac ggatgcgcta
     1621 ctcctccgag gcgctcgcgg acgccctcaa ccggatcctc gaagggatct tcccctaccg
     1681 ccggggcaag atctattccc gccacggcac ggcgaacgcg gtgagcggag ccctgctggt
     1741 ggaccagagg cggtagggga gagcagcgtg aactccacct ttgccccgat cgagctgggc
     1801 aagcgcggac tggtggccac cacccagggg atccagaccg tcgggcacaa catgtcgaac
     1861 gcggccgtgg agggctacag ccggcagcgg gtcgagctga cggccagccc cccgctggat
     1921 gagccccagc tcaaccgcga ggaccggccg ggtcagctcg gtcagggcgt ggagaccgcc
     1981 cgcatcgtcc gcctgaagga catgctcctg gaggggcgga tcgtctctga gcagaacgtc
     2041 gaggggtact gggacgcccg ggacaagtac gtcctcatga tggagcagat ctacaacgag
     2101 cccaccgagc attccgtgcg gaccctcatg gacaagttct gggactcctg gcaggagctc
     2161 tccctgaatc ccacggagat ggcctcccgc agcgcggtgc ttcagcgcgg caaggcgctg
     2221 ctggacggcg tgcagagcca ctaccagcag cttcactcgg tccgggacat gatcgagggg
     2281 gacgtccagg gcgtggtgac gcaggtcaac gagatcacca agagcatcgc gggcctgaac
     2341 ggggagattg tccgagccca ggcgctggga gacaacccca acgacctcct ggaccgccgg
     2401 gacctgctgg tgggcaagct ttcgaccctc atcgacatca gcgtgcgcta caacgatccg
     2461 gcgggagagt tcgtggtgac caccggcggc acccacatcg tccagggcca gcattccgag
     2521 acgatcgccc tctccgcgaa caagtccaac gagggctatt cggacctggt gtgggaggat
     2581 acgcgccagg ccgccatcgt ccgcggaggc aagcttgcct ccctgatcga gctgcgggac
     2641 ggcgatgccc gatcggagat ccagagcctg gaccagctcg ccatgcactt ctcggacctg
     2701 gtcaacgaga tccaccgcca gggctacggg ctgaacgggg acacgggcaa ggacttcttc
     2761 gtccagcgcc cgttcgtgac caacgtgacc ggcgcctacg attcgaatgg ggatggggcg
     2821 ctcgactcga cgtacctctt ccgcgtcacc ggcgccaacg cgcttcagcc gaaggagcag
     2881 atcgggcttg cggggaccct cacgctgccg ggccacgacg gcgacgtgaa gatcgactat
     2941 caccccacgg acaccgtgga tgacctcatc acccggatca acctgtccgg gtccgaggtc
     3001 gtggcgcggc tgaactccga gggaaagctc tccctcaagg cggccccggc ggccaacacg
     3061 ggctatccgg acttcgtcat ccggggcctg gaggactccg gcccgttcct ggtgggatac
     3121 gccggcgtgc tcaaggcctc gggacccgcg ggggcctact cgtggaaccg gccggacgcg
     3181 gtcaccacgc tggccggccc ggacacccag tacgccgtgg cgccgctcgc tcatcccgcg
     3241 ggctggatgg agatcaaccc cctcctggcg caggacccgg gatccatcgc ggccgcggcg
     3301 ggcgtcgacg gcaggccgga gggcgtggac aacggcgccg cggccctggc catcgcccag
     3361 cttcgcacca cgccggtcat ggtgggcgcc agctcgacgt tcgacggatt cctggcggac
     3421 cgcgtggcct ccatcgggct gaagggggag gaggccaagc agagcctgga cacgtcgcgg
     3481 ctcgtcatga agaacctgac ggatacgcgg gagtcgatct ccggggtcaa cgtggacgag
     3541 gagctgacgc agatgatcac ctatcagaac ggctacgccg cgatcgcgag gttcgtcacc
     3601 accgtggatt ccttgctgga catcatcatc aatcgaatgg gcgtgtaagg aaggcgtgtg
     3661 aatgaagagg atcagctccc agcttcccgc gagtgacagc agcttctatc tccgtcagcg
     3721 ggaagtcgaa atgaacgata tgagcaacaa gatcggcgcg cagagcacca tcaaggacct
     3781 gcgggacgat cccctgggcg cggcgaaatc ggtgcggctg cagtcggaga tcttccggct
     3841 ggaccgctac ggcaagaaca tccaggacat ccgcgcgcgg ttcaccacgg ccgaggggaa
     3901 cctccagagc gccatggata tcctgcagag ggtccgggag ctcgccgtcc agggggccaa
     3961 cggaacgctg gacacccagc agatgggcta catcggagac gaggtggacc agctcctggg
     4021 agagctcctt tccatcggca actcca
//
LOCUS       DASRZS010000623         3450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2261026, whole genome shotgun sequence.
ACCESSION   DASRZS010000623 DASRZS010000000
VERSION     DASRZS010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2261026"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="VFH83_15195"
     CDS             <1..1624
                     /locus_tag="VFH83_15195"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET6487772.1"
                     /translation="ASCATTRGSTLGQVISTEAKPNQPAPASSSNPPSQPSQTTTTTT
                     EPKKGLEVDTDPDNAEVWIDGSFRGLSPYLASDIPIGWHQVTVRKQGYYDTTTWVEFK
                     TDYMLYQATLTRITGFLQLQVTPSDATASVGGTRMQPGLLQLPVGSYDVVVRDFGYTD
                     YRATVTIQEKAITALSVVLNPAPFAITAFALPKDAVNPDSPGLLGSLEAGFSVSGPGT
                     GRLVVTDSKSDEVFSQPLARFTTWDQNVRWNLRDSAGHVLGDGTYVLAIVAQGDDGSQ
                     SIRREATFRVDRTLKVAARSVWSGGAGLLYAPVAETLPKGDFQAEVLVAAHADPSLFR
                     APAAVGARVGLGDRLEVDAVAGLIPSSVATPLFAGVAARWNLLSPAGEFGTALAVQTK
                     LSVQLDPGPSGSSVLLTDTFANFTGIGVELPFQLDLGRVSVLLTPGVIGSLWHPYGSS
                     DATPQAWLYLRAGALLDLGDVTAGVSVSTRTEQLPGGVTLLATPIPYEAAAEVHWLVP
                     GTRLLLSALLAEEYQDSNNYYFMGGGGLGFLY"
     gene            complement(1642..2019)
                     /locus_tag="VFH83_15200"
     CDS             complement(1642..2019)
                     /locus_tag="VFH83_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6487773.1"
                     /translation="MKVRFYCESCGSEVGKSDAVCPSCGKSFAAVRCPQCGFSGKASA
                     FARGCPVCGYLHENRAVPVAPEQAEGGTPAPRGEERGSRRGTARSQTGRRGRGLPPGF
                     YRWAIVVLLVVLIGLTVLFLSRP"
     gene            2172..3224
                     /locus_tag="VFH83_15205"
     CDS             2172..3224
                     /locus_tag="VFH83_15205"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HET6487774.1"
                     /translation="MESVLIGLARTGRTTIFNALTGQDMAVGDASLARRQPYLSEVKV
                     PDDRLDKLAALFHPRKLTHAAVLFKDPQLDQDDKGNISATSLAEVRKADAIAVVVRAF
                     VSEGVEPAFPGATPLGELRRILDSLVFGDLEISEKRLARLDKEAKRDTREYKVLQQIV
                     ATLGTGKPLGAGFLTSEDLRLFAGFGFLTTKPLFVVVNTGEKALPHEDLVKEAAAQGI
                     HVFPIRGDMEMEISRLSPEDQLAFLQDLGLEEPARNRFLRHVYSTLNLVSFFTVGDDE
                     CKAWSIRQGTNAVGAAGEIHTDLAKGFIRAEVAAWQDVVEYGDLTKAKKGNKVRLEGK
                     DYVVRDGDVVLIRFNV"
BASE COUNT          580 a         1151 c         1158 g          561 t
ORIGIN      
        1 cgcctcgtgc gccacgaccc gcggctcgac cctgggtcag gtgatcagca cggaggccaa
       61 gcccaaccag cctgctccag cgagcagcag caatccgccg tcccaaccca gccagacgac
      121 caccaccacg acagagccca agaagggcct ggaagtcgat accgatccgg acaacgccga
      181 agtgtggatc gacggttcct tccgcggcct ctccccctat ctggcttcgg acatacccat
      241 agggtggcac caggtcacgg tccgcaaaca gggatactac gatacgacaa cctgggtgga
      301 gttcaagacc gactacatgc tctaccaggc gacgctgacc cggatcaccg gattcctcca
      361 gctccaggtc actccctcgg acgccacggc ctccgtggga ggcacgcgga tgcagcccgg
      421 gctgctgcag cttccggttg gaagctacga cgtggtggtc cgagacttcg gctatacgga
      481 ttaccgggcg acggtaacca tccaggagaa ggccattacg gccctttcgg tggttctcaa
      541 cccggccccc tttgccatca cggcttttgc gctgcctaag gacgcggtga atccggacag
      601 ccccgggctt ctgggcagcc tggaggccgg attctccgtg tcgggacctg gcacggggag
      661 gctcgtagtc accgactcca agtccgacga ggtcttcagc cagccgctcg cgcggttcac
      721 cacctgggat cagaacgtcc ggtggaacct tcgggactcc gccggtcacg tccttgggga
      781 tggcacctac gtgctggcca tcgtggccca aggcgacgat ggctcgcagt ccattcgacg
      841 cgaggcgacc ttccgcgtgg atcgcacgct gaaagtcgcg gcccgaagcg tctggagcgg
      901 cggtgccggg ctgttgtacg ctccggtcgc ggagacccta cccaagggag atttccaggc
      961 cgaggtgctg gtcgcggctc atgcggaccc gtccctgttc cgggcgcccg ccgcggtcgg
     1021 agcccgggtc gggctcgggg accgactcga ggtggacgcc gtggctggac tcatccccag
     1081 ctccgtggct accccgctct tcgccggcgt cgcggcccgg tggaacctcc tctcccccgc
     1141 cggagagttc gggacggcgc tggcggtcca gaccaagctc tccgtgcagc tggaccccgg
     1201 acccagcggc agctcggttc tcctcaccga cacgttcgcc aacttcaccg gcatcggcgt
     1261 cgagctgccc tttcagctcg atctgggacg agtgagcgtg ctcctgacgc caggggtgat
     1321 cggctccttg tggcatccct acgggtcctc ggatgcaacc ccgcaggcgt ggctgtacct
     1381 gagggctggg gccctccttg acctcggtga cgtgacggcc ggtgtgtcgg tcagcacgcg
     1441 gaccgagcag ttaccgggcg gagtcacgct gctcgcgact cccatcccgt acgaggcggc
     1501 ggcggaagtc cactggctgg tgccgggcac gcggctgctg ctatccgcgt tgctggcgga
     1561 ggagtaccag gactccaata actactactt catgggtggg ggtggactgg gattcctcta
     1621 ctgatcgtcg gctgcggcgc gtcacggcct gctgagaaag agaaccgtga gaccgatcag
     1681 cacaacgagg aggaccacga tggcccatcg atagaacccc ggcggaaggc ctcgaccgcg
     1741 ccggcccgtc tggctgcgtg ccgtgccgcg ccgggagccg cgctcttcgc ctcgaggcgc
     1801 gggcgttccg ccctctgcct gctccggcgc cacgggaacc gcgcggttct cgtgcaggta
     1861 gccgcagacc ggacagcctc gggcaaacgc ggacgccttt ccggagaagc cgcactgggg
     1921 gcagcgcacc gcggcaaaag acttgccaca gcttggacag acggcgtccg attttccgac
     1981 ttcgcttccg cagctttcgc agtagaatcg taccttcatg gacgttgcaa cgccccgggg
     2041 cctcctttat acttcccaag gcggcggagg agcaagggag gtcgccggcg gccgttccga
     2101 agccgtgcag gcgtcccacc gcatcgccca gggctcgtac gcgggccgcc atcgaccttg
     2161 gaggagattg gatggagagc gtactcatcg ggctggcccg aaccgggcgt actacgatct
     2221 tcaacgcgct cacgggacag gacatggccg tgggagacgc cagcctcgcg cggcggcagc
     2281 cctacctctc tgaggtgaag gttcccgacg atcggctgga caagctcgcg gcgctgttcc
     2341 acccgcgcaa gctgacccat gccgccgtgc tctttaagga ccctcagctc gaccaggatg
     2401 acaaggggaa catctccgcc accagcctgg cggaggtgcg caaagcggac gccatcgcgg
     2461 tcgtcgtgcg cgcgttcgtg tcggaaggag tcgagccggc gttcccaggg gccacaccgc
     2521 tcggtgagct tcgacggatc ctcgattcgc tggttttcgg cgacctcgag atcagcgaga
     2581 agcggctggc ccggctggac aaggaggcca agcgcgacac ccgggagtac aaggtgctcc
     2641 agcagatcgt cgcgacgctg ggcacgggaa agcctctggg cgcgggcttt ctgacctccg
     2701 aagacctgag actgttcgcc gggttcgggt tcctgaccac gaagcccctg ttcgtggtcg
     2761 tcaacaccgg agagaaggcc ctgccgcacg aagacctcgt gaaagaggcg gcggcccagg
     2821 gcatccacgt ctttcccatc cggggggaca tggagatgga gatctcccgt ctgtcaccgg
     2881 aggatcagct ggcgttcctt caggacctgg gtctggagga gccggcgcgc aaccgctttc
     2941 tgcggcacgt ctattctacg ctgaatctcg tgagcttctt caccgtcggg gacgacgagt
     3001 gcaaggcgtg gagcatccgc caggggacca acgcggtcgg ggccgcggga gagatccata
     3061 cggacctggc caaggggttc atccgcgccg aggtggccgc ctggcaggac gtggtcgagt
     3121 acggcgacct gaccaaggcc aagaagggca acaaggtccg cctggagggc aaggactacg
     3181 tcgtgcgtga cggggacgtg gtcctgatcc gtttcaacgt gtagcgtcgc caggggcggc
     3241 tctccagcgg ccgcatgcgg cgcggtccag gcgccgcggt tggcgctcca gcggccgcgg
     3301 cccggagaac gagctcctac tgcgcggcgc tgccgccgct tccaaaggtc agggtcgcga
     3361 aggcaaactc ctcatagctg gcatgggcct tgtcgtacca gcaggaggtg aggtccgccg
     3421 tggtcgtcgg acccagggcg gcctgcaggc
//
LOCUS       DASRZS010000624         4005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2669215, whole genome shotgun sequence.
ACCESSION   DASRZS010000624 DASRZS010000000
VERSION     DASRZS010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2669215"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..616)
                     /locus_tag="VFH83_15210"
     CDS             complement(137..616)
                     /locus_tag="VFH83_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487775.1"
                     /translation="MAIGDLELAPQMDQLYFPDRIVGTARRSWRKLPPVARTVDILEI
                     QTGVRSPYELLSSAVAADVEYLRWRVYRACDIAEDLGIHKIVLSLPGRRMAPSGRGEP
                     SAALRSLLREIVGYDTQRTFVLAIGESFQESGFRPETENDLMHLVRAGRLEVRRTSS"
     gene            795..1769
                     /locus_tag="VFH83_15215"
     CDS             795..1769
                     /locus_tag="VFH83_15215"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET6487776.1"
                     /translation="MDKQFYFFNMSYEQVPHAPGALFRTFFISIGYAGMHWHKEMEML
                     LVLDGSISVTSSGQTFTLQAGDLFWVNAWEAHSLLETADDNLLLVLQIDPAAASGASA
                     EFHDRQYELTPARPLPLRHARNVCRAMARLLTLSAEQPTAHALSSMEALYSILASIRQ
                     GAAAHHPMSGRRRDGQVAERTKEIVDYLNRRYREPISLKRMSAHFRLSPFYVSHLIRL
                     QTGFSFQEHLSFIRLHHAVDLMMTTDLRLIDIALESGFSDPKYLNKYFRLLFGMRPSE
                     LRRKENWKQVIKSRFGQGSLDVATLGSYLAPYLSQVDEGGSASTAVRT"
     gene            1839..1932
                     /locus_tag="VFH83_15220"
                     /pseudo
     tRNA            join(1839..1879,1899..1932)
                     /locus_tag="VFH83_15220"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /pseudo
                     /anticodon=(pos:1876..1878,aa:Trp,seq:cca)
     gene            2141..3568
                     /locus_tag="VFH83_15225"
     CDS             2141..3568
                     /locus_tag="VFH83_15225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6487777.1"
                     /translation="MKYKWTALTVTTVGALMSSLDTTIVIIGLPTILNDLHATIVHGI
                     WVITGYQLMMTVLLVLLGRLADMHGRVRLYNIGFVIFTIGSLLAGLSRNGAQLVAFRF
                     IQGSGAALLSANSAAIITDAFPPGELGMALGTNMMAFNVGAVVGYTLGGAMITFLGWR
                     SIFFINVPVGIFGTVWAYKRLREVSIRASGQGFDYLGSALYCAGLAVILLALTVGDPT
                     SARNVLVLVAGLVVFAVVILVERRQRFPTLDLALFRIRVFAAGNLASFLNAVAFNCGP
                     FLRSLYLQLILGYSPLQAGLALIPMEVMVFVLSPISGRLSDRVGGRVLSSVGLAMNAS
                     ALFWFSTLDQHSSYASVLVSLVLFGFGRALFISPNSSSVMGAVPPEKRGVANGVRMTL
                     NMTGGVLSVPLSLLLMTFVMPYGRLAEIVGTNQLASGGELLTFLHAINHACLILGMVI
                     VVAIIPSLLRGPRTTSHPQSIVHDA"
     gene            complement(3623..>4005)
                     /locus_tag="VFH83_15230"
     CDS             complement(3623..>4005)
                     /locus_tag="VFH83_15230"
                     /inference="COORDINATES: protein motif:HMM:NF042842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6790 family protein"
                     /protein_id="HET6487778.1"
                     /translation="LAAPVLVAGKWFVFWAVGARLFLAGLRQVLQPRFTAETILGIKG
                     DAPLHIVQELGFANISIGALGLLSILRGAWVMPAAIAGCLFYGLAGIRHLTQRNRNAL
                     ENTATVSDLFMFAVLAAYAVIALA"
BASE COUNT          692 a         1287 c         1273 g          753 t
ORIGIN      
        1 catagaggtg tacctcggat ttccgagcgc acactcaagc agagcacttt cacgtattgc
       61 gtgagcgtgg cggccgggca acacgtcgag gccgcaggcc gcggactccc ctgacggggg
      121 cagcccgtgt cgcagctcag ctcgacgtcc gtctcacctc caggcgcccc gcgcggacga
      181 ggtgcatgag atcattctcg gtttcagggc ggaagccgga ttcctgaaag ctctcaccta
      241 tggccaggac aaaggtgcgc tgcgtgtcgt agccgacgat ctccctgagg agcgagcgca
      301 acgcggcgga gggttctcct ctgcccgacg gcgccatgcg ccgacccggg agggagagga
      361 cgatcttgtg aattcccagg tcttcggcga tgtcacacgc ccggtagacg cgccaccgga
      421 ggtattcgac gtccgcggcc accgcggatg acaggagctc gtaggggctg cgcacgccgg
      481 tctggatttc caggatgtcc accgtccggg caaccggcgg cagcttgcgc cagcttctcc
      541 tggcagttcc aactattcta tcagggaaat agagttggtc catttggggt gccagctcca
      601 gatcgccgat tgccaaccgg ccctccgcgc ctggccctgg ttccatgaca aggggcggga
      661 gacacggatc acgttcccct ctctccatga tgttgcccat gtcctggatc attgacgcga
      721 ggcagcgggg cgtccatcat gatcttgtgg ccggcgaccg acaatcttgc cattgtgtcc
      781 ccggaccagg agtcatggac aagcagttct atttcttcaa catgtcctac gaacaggtcc
      841 cccacgcccc gggtgcgctc tttcgaacgt ttttcatttc catcggctac gccgggatgc
      901 actggcacaa ggagatggag atgctgctcg tcctggacgg cagcatctcg gtcaccagca
      961 gcggacagac cttcacgctg caagccggcg acctgttctg ggtcaatgcg tgggaggcac
     1021 acagcctgct ggagaccgcg gacgacaacc tgctgctggt cctgcagatc gatcccgccg
     1081 cagcctcggg cgcgtccgcc gagttccatg accggcagta cgagctgacg ccggcgcggc
     1141 cgctgccgct gcggcatgcc cggaacgtct gccgcgccat ggcgcggctg ctgacgctga
     1201 gcgcggagca gccgaccgcc cacgcgctga gctccatgga ggcgctgtac tccattctcg
     1261 cgagcatccg tcagggcgcc gccgcgcacc acccgatgag cgggcgacgt cgggacggcc
     1321 aggtggcgga gcggaccaag gaaatcgtgg actacctgaa tcggcgctac cgggaaccca
     1381 tttcgctgaa gcggatgtcg gcgcatttcc ggctgagccc cttctacgtg tcccacctga
     1441 tccgcctgca gaccggattc tcgttccagg agcacctgag cttcatccga ctccatcacg
     1501 ccgtggacct catgatgacg acggacctga ggctgatcga tatcgccctg gaatccggct
     1561 tctctgatcc caagtacctg aacaagtact ttcgcctgct cttcggcatg cggccctcgg
     1621 agctgcggag gaaagagaac tggaagcagg tgatcaagag ccgcttcggc cagggctccc
     1681 tcgacgtggc gaccctgggt agctacctgg cgccgtatct ctcccaggtc gatgagggag
     1741 gcagcgcctc gactgccgtg cggacgtgac ctgaaattcc cggacttctt gacacttgtc
     1801 ctcctccggt ggaaaattaa gatagataca cgcgatctgc tgcggggcct cgacgagtcg
     1861 gcggacttgc ctctgccacc ctgagaggac ggcgcccgaa agggtgcgct ggttcaaatc
     1921 cagtccgcgg catctttctg gaaacggaca ttcagccctt tcggtttggc gaccccctcc
     1981 aaaaggcccg agggtaccac gcggggtacc cacacctacc gaaaatggcc ttttcgggag
     2041 gtacggcctt gtcaacgaag aagctctacc tggtcgggac acgggtccgg ctcgccgaac
     2101 cgaccgaaga tgcgggtaca ccggcacgcg tggagctggc atgaaatata agtggaccgc
     2161 actcaccgtc accaccgtcg gtgcgctcat gtccagcctg gacaccacga tcgtcatcat
     2221 cgggctgccc accattctca acgacctgca cgcgacgatc gtgcacggca tctgggtcat
     2281 caccggctac cagttgatga tgacggtgct gctggtcctc ctggggcgtc tggcggacat
     2341 gcacggccgg gtgagactgt acaacatcgg gttcgtgatc ttcaccatcg gctccctgct
     2401 ggccggcctc tcgaggaacg gcgcccagtt ggtggcattc cgattcatcc aggggtcggg
     2461 tgcagcgcta ttgagcgcga actccgcggc tatcatcacg gacgcattcc cgccgggaga
     2521 gctggggatg gcgctgggta ccaacatgat ggcattcaac gtgggcgccg tcgtgggcta
     2581 taccctgggc ggggcgatga tcacgttcct gggatggcga tcgatcttct tcatcaacgt
     2641 gccggtcggg atcttcggga cggtctgggc gtacaagcgg ctgagggaag tgagcatacg
     2701 ggcatcgggc cagggattcg actacctggg gtcggccctc tattgcgcgg gtctcgcggt
     2761 gattcttctc gcgctgaccg tcggggaccc cacttccgca cggaacgtcc tggtcctggt
     2821 cgccgggctc gtggtcttcg ccgtcgtgat cctggtcgaa cggaggcagc ggttccccac
     2881 gctcgacctg gcgttgttcc gcatacgggt ctttgccgcg ggcaacctcg cgagcttcct
     2941 gaacgcggtg gcattcaact gcgggccgtt tctccgctca ctctacctgc agctcatcct
     3001 cgggtacagc ccgctccagg cggggctggc cctcatcccg atggaggtca tggtcttcgt
     3061 cctcagcccg atcagcgggc gactctcgga ccgggtgggc ggcagggtgc tcagctccgt
     3121 ggggctggcc atgaatgcct ccgcgttgtt ctggttttcg acgctggacc agcattcaag
     3181 ctacgcttcg gtgctcgtga gcctggtgct cttcggtttc ggcagggcct tgttcatctc
     3241 ccccaactcg agctcggtga tgggcgccgt gcccccggag aagcgcggcg tagccaatgg
     3301 cgtcaggatg accctgaaca tgaccggcgg ggtcctgagc gttccgctgt ccctgctcct
     3361 gatgacgttc gtgatgccgt acgggcggct tgcggagatc gtcggcacca accagctcgc
     3421 aagcggcggg gagcttctca cgttccttca tgccatcaat cacgcctgcc tgatcctcgg
     3481 catggtcatc gtcgtggcca tcatcccgtc cctgctcagg ggtccgcgaa ccacgtcaca
     3541 cccgcagtcc atcgttcacg acgcgtaggc gggtagttcg tgtcgatctg cgtggtagcc
     3601 ccgggcgctc ataggtggag gatcaagcaa gggcgatgac ggcgtacgcc gccagcacgg
     3661 cgaacatgaa caggtccgag accgtggcgg tgttctccag cgcgttgcgg ttgcgttgcg
     3721 tgaggtgccg gatgccggcc agaccataga acaggcaccc ggcgatagcc gcgggcatga
     3781 cccacgcacc gcgaaggatg ctcaggaggc caagggcacc gatcgagatg ttcgcgaaac
     3841 ccagctcctg gacgatgtgc aagggcgcgt cccctttgat cccgaggatg gtttcagcgg
     3901 tgaagcgagg ctgaagcacc tggcgaagcc cggccaggaa cagcctggcg ccgaccgccc
     3961 agaagacgaa ccatttccct gcgaccagaa cgggcgccgc gaggg
//
LOCUS       DASRZS010000625         3067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4967230, whole genome shotgun sequence.
ACCESSION   DASRZS010000625 DASRZS010000000
VERSION     DASRZS010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4967230"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VFH83_15235"
     CDS             <1..457
                     /locus_tag="VFH83_15235"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6487779.1"
                     /translation="KRLLDSITLNHAVDPVSAEWSRRTGGAVFELHCYALPRDGLTDG
                     EVRNGLLQELTDHLPDLAGASVVHEHLQVRHDFTAFHTGMHGTRPGPVTALPQLVLAG
                     DWVRLPIPAMLMEAACTSAIIAANAVLDRVGLRQEPFWSVPETGLFARS"
     gene            552..1541
                     /locus_tag="VFH83_15240"
     CDS             552..1541
                     /locus_tag="VFH83_15240"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6487780.1"
                     /translation="MVQKEKWFDRKFPSGLPLATLANTLERLKGTPARLEERLAGVGA
                     TALTRKVGGTWSAQENAGHLLELESLWLTRVSELLSGRGEMSPADLTNRGTDDADYNA
                     RDVSLVLRAFREKRMRLVSRIEAADDAALGHTALHPRLRTPMGIADFAWFVAEHDDHH
                     LARITELLRTIEAQETPTSASAEASGQPSGPASPDQRGMIALDTTTMPWEERYQAALG
                     KSNFRKLLLQDPDTGMEVRLLRYPAGFTATWHTHPCGHGMFVLEGTLVTQDGNHGPGS
                     FVWFPQGMVMEHGATAQADVVMLFITNKKFAIHFVERPAAAPNVVYSPFPKVT"
     gene            complement(1514..>3067)
                     /locus_tag="VFH83_15245"
     CDS             complement(1514..>3067)
                     /locus_tag="VFH83_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487781.1"
                     /translation="APSQDVHRAAPVNVPVLPVSDVQVKPTPNAVSDAEVSEAQAALR
                     DASAWGAEYYEPQMYAKAQATLKAALAARYHDPAQCRSLLSGAAEAASSAKGAALAAY
                     QDDVKSRFEASRAKLVEIGADRAFPDEYASLQSGMDATIGLFANGSYWDARVKAYGTL
                     KGMSDLYERVHGLSIWLRDARIRVGNAISDARALDAPRWAPADMQDAEQKYHDALTRM
                     QAGDLTAAVDSMRAAGQIAARLPLLKDRIDKPGAGTPPVAGGSVPPAAGAPPATQKPR
                     GQGPDQRLQQESAEKSLSGQRVRIADMSMSSLGAAQRLYSDFSAAVARFDVVAAEGLR
                     DAGIMEKVLAGLNDRWEAAVSRSGYFGFIFGDRVEMVRDLGTYPGERGLLRSPYAAQF
                     RLLGTRFLVNLVLCHVEASKDNQATAAAVAQLADIYRYFENLTGNRGVTLLLVGGLGD
                     LPEPTSSDSLMPQGEVVSLRTNVTATAGAHNQGQRMFASAALQPLVAESGSSASTPPF
                     AYVTLGKGE"
BASE COUNT          502 a         1007 c         1027 g          531 t
ORIGIN      
        1 gaagcgtctg ctggattcca tcaccctcaa ccacgcagta gacccggttt cggcggagtg
       61 gtcccggcgg accggcggcg ccgtgttcga gcttcactgc tatgcgctac cccgagacgg
      121 gctcacggac ggcgaggtcc gaaacggact tctccaggag ctgacggacc atcttcccga
      181 tctggccggc gccagcgtgg tgcacgagca ccttcaggtc cgtcacgact ttacggcctt
      241 ccatacgggg atgcacggga ccaggcctgg gccggtcacc gcgctgccgc agctcgtgct
      301 ggcgggtgac tgggtccggc tgccgatccc cgccatgctg atggaagctg cctgcacgtc
      361 cgcgatcatt gctgcaaatg cggtcctcga tcgcgtgggg ttgcgccagg aaccgttctg
      421 gtcggtcccc gaaacggggc tattcgcccg atcgtgacca ccgatgccgg acaacccgag
      481 gaggccgatc cgcgcgttga tcccggacgg gggcgcttga gcggcagccg tccgggcagt
      541 agtttcatgc catggttcag aaggagaaat ggttcgatcg gaagttcccc tcgggcttac
      601 cgctcgccac gcttgcgaac actctggaac ggctcaaggg gactccggcc cggttggagg
      661 agcgcctcgc gggggtcggc gccacggccc taacgcggaa ggtcggtggc acctggtccg
      721 cgcaagagaa tgccggtcac ctgctcgagt tggagtcgct ctggttgacg cgcgtctcag
      781 agctcctgtc gggtcgcggg gagatgtccc ccgcggatct gacaaaccga gggaccgatg
      841 acgccgatta caacgcccgc gacgtctccc tggtccttcg cgcctttcga gagaaacgga
      901 tgcggctcgt gtctcggatc gaggccgccg atgacgccgc cctgggccac accgcactcc
      961 atccaaggct tcgcactccg atggggattg cggactttgc ctggttcgtg gcggagcatg
     1021 acgaccatca tctggcgcgg atcaccgagc ttctgcggac catcgaggcg caggagaccc
     1081 ctacctcagc ctcggccgag gcgagcggcc agccgtccgg gccagcctca ccggaccagc
     1141 gggggatgat cgccctggac acgacgacca tgccgtggga agagcgctat caagccgccc
     1201 tcggcaagag caacttccgc aagctcctgc tgcaggatcc ggacaccggc atggaggtcc
     1261 ggctgcttcg ctacccggcc ggcttcacgg caacgtggca cacccatccc tgcggccacg
     1321 gcatgttcgt gctggaaggg acgctggtca cccaggacgg caaccatggc cccggcagct
     1381 tcgtgtggtt tccgcagggc atggtgatgg agcacggtgc gaccgctcag gcagacgtgg
     1441 tgatgctgtt tattaccaac aagaagttcg cgatccactt cgtcgaacga cctgcggccg
     1501 ctccgaacgt ggtctattcc cccttcccca aggtgacata ggcgaaggga ggcgtggagg
     1561 cgctggatcc tgattccgcg accagcggct gcagtgcggc gcttgcgaac atacgctgtc
     1621 cctggttgtg ggcgccagcg gtggccgtga cgttggtgcg cagggagacg acttctccct
     1681 gaggcatcag ggaatcggag gaggtcggct ccggcagatc accgaggccg ccgaccagca
     1741 ggagggtaac cccacggtta ccggtcaggt tctcgaagta gcggtagatg tcggcaagtt
     1801 gagcgactgc ggccgcggtg gcttggttgt ccttgctggc ttcgacatgg cagaggacaa
     1861 ggttcacgag gaagcgagtt cccaggagcc ggaactgcgc ggcgtagggg gagcggagaa
     1921 gaccacgttc gcccgggtac gtgcccagat cccggaccat ttcgacccga tcgccgaaga
     1981 tgaatccgaa atagccgctt ctgctcacgg cagcttccca gcggtcattc aacccggcca
     2041 acaccttttc catgatcccc gcgtccctga gaccctcggc ggccacgacg tcgaaccggg
     2101 ccactgcggc tgagaaatcg gagtacagcc tctgcgcggc tcccagggag ctcatgctca
     2161 tgtcggcaat ccgaacccgc tggccagaga gggacttctc tgcagactcc tgctggagcc
     2221 tttggtcggg accttgtcct cgcggcttct gcgtagcggg aggtgcccct gccgcagggg
     2281 gaacggagcc accggctacg ggcggcgtgc ctgccccggg cttgtcgatt cggtccttga
     2341 ggagggggag tctggcggcg atctgaccgg cagccctcat ggagtccacc gccgcggtca
     2401 ggtcgccggc ctgcatccgc gtcagggcat cgtgatactt ctgttcggcg tcctgcatgt
     2461 ccgcgggtgc ccaccgaggc gcgtcgagtg cccgtgcgtc gctgatggcg ttccccacgc
     2521 ggatccgcgc gtcccgcagc cagatcgaga gaccgtggac cctctcgtag aggtcgctca
     2581 tgcccttgag cgtgccgtag gccttgacgc gggcatccca gtaggacccg ttcgcaaaga
     2641 ggccgatggt ggcatccatg cccgactgca gcgaagcgta ctcgtccggg aaggctcggt
     2701 cggcgccaat ctccacgagc ttggcgcgcg acgcctcgaa ccggctcttc acatcgtcct
     2761 ggtaggccgc cagtgccgct cccttcgctg agctcgcggc ctcggcggcc ccggacagaa
     2821 gagaacggca ctgagccggg tcgtgatacc gcgcggccag cgccgccttg agcgtcgctt
     2881 gcgctttggc gtacatctgg ggctcgtaat actccgctcc ccacgccgag gcgtcgcgaa
     2941 gcgccgcctg cgcctctgag acttcggcat cgctgacggc gttcggagtg ggtttcacct
     3001 ggacgtcact caccggcaag accgggacgt tcactggagc ggcccggtgg acgtcctggg
     3061 agggggc
//
LOCUS       DASRZS010000626         6106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1896774, whole genome shotgun sequence.
ACCESSION   DASRZS010000626 DASRZS010000000
VERSION     DASRZS010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896774"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFH83_15250"
     CDS             <1..239
                     /locus_tag="VFH83_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487782.1"
                     /translation="SSNQVTATVTFNNESDGSYTFKVWYDADNDGSSYLESTYYNYYY
                     YNTYGYVNGSTVSNVGINGGVTANVTVNLYEYTY"
     gene            complement(296..1669)
                     /locus_tag="VFH83_15255"
     CDS             complement(296..1669)
                     /locus_tag="VFH83_15255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6487783.1"
                     /translation="MQNVGQARDVAVLSLLSVQAILVAARLPSCDRRAQPLCLIGVLG
                     LLAGTVACAGNPVAYAICVLGVLWGGLVLSGPQSRRRPGWAALGGAFLAAEALSFALG
                     LTGTLSFRAFHLLGLAFLGGASLVRLARQAARTRAPYLLVTLAAAAGWFAAVAAPAVA
                     RVVGRSVPDCSAWLAGGLALCTGWLVFQERYPLRTAPGSAHARLQRPDEALGPQGRLM
                     ATAHLAMAAAHEFRSTLSTVRLAAGHALSLADSAAKDESLKLLLELAEAGERSAVSVL
                     LRLAREGREPECTLDALRDFADLVRKTGTPFRAEGILVRPHLAEGVRFVARKGEVEQI
                     LLCLLRNAIEAYRGAGRDRDAGRDRGAGRDRDAGPHHDAGEVVVEARCQGETAVLEVS
                     DRAGGVPIDAEQDLFSVGSSRSASTGLGLYLARGLAQANGGFLEYLPLDGGSLFRLTF
                     PLADDAL"
     gene            complement(1686..2816)
                     /gene="dnaJ"
                     /locus_tag="VFH83_15260"
     CDS             complement(1686..2816)
                     /gene="dnaJ"
                     /locus_tag="VFH83_15260"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET6487784.1"
                     /translation="MAKRDYYEVLGVAKGASRDEIRKAYRQQAIKFHPDRNPGSKDAE
                     EKFKEATEAYEILADDGKRQAYDQYGFAGVKGMGQPNAQDFSTIFQGFEDIFGDSGGL
                     FDMFFGGSGRRRSSSRGGSQRGSDLRYDMEVEFTEAVFGAKREVTYERSVRCDVCGGT
                     GSDKGGGRRLCPTCGGSGQVRRNSGFFSIASTCPHCNGEGEIIDRPCGECRGTGAVRG
                     RKTLTITIPAGIDDGKRLSLGGQGDAGTRGSPDGDLYVVIHVKPHELYERDGNDLYCA
                     IPISVTQAALGAEIGVPTLDDKTVRLTVPAGTQNGKVLRLRGEGVPELHAPARRGDLY
                     VKILVRIPTKLSSRAKDLLKELASTNGEEKNPKPVPLSELKQ"
     gene            complement(2820..4796)
                     /gene="dnaK"
                     /locus_tag="VFH83_15265"
     CDS             complement(2820..4796)
                     /gene="dnaK"
                     /locus_tag="VFH83_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET6487785.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGQPVVIQNSEGQRTTPSIVAFTE
                     SGEHLVGQPAKNQMITNPESTVYSIKRFMGRRMEEVGEEAARVPFRVVDGGNDVRVEV
                     RGTRYSAPEISAAILQKMKKTAEDYLGETVTQAVVTVPAYFNDAQRQATKDAGRIAGL
                     DVKRIVNEPTAAALAYGLDKGGREETIAVYDLGGGTFDISILELGDGVFEVRSTNGDT
                     HLGGDDFDQRIIEWMAAEFKRESGIELTKDRMALQRMKEAAEKAKIELSSAQTTTINL
                     PFITADRTGPRHLTYTLTRARLEQLLADLFERTRGPCLQALRDAGLTRSDIDHVILVG
                     GSTRIPAVQAIVRDIFDREGSKGVNPDEVVAIGASIQGGILGGDVKDVLLLDVTPLSL
                     GIETLGGVFTKLIERNTTIPTRKSQTFSTASDGQTAVSIHVLQGEREMASQNRTLGRF
                     DLVGIPPAPRGVPQVEVAFDIDANGIVHVSAKDLGTGKEQKVRIESSSGLREDEIRRM
                     VKEAEANAVEDRVERERAEVCNEADSLLYSSERSLRDLGTRVSAEDRAAIEAASRELR
                     AAVTSRDVSLVRDRMDSLRRASYRMAEELYKDRSSSGTDASGPSSNGGGSSTGSDGPG
                     PSPGRVHAGKRGSRKPQRPAQDDADYEIVDDEKP"
     gene            complement(4805..5488)
                     /gene="grpE"
                     /locus_tag="VFH83_15270"
     CDS             complement(4805..5488)
                     /gene="grpE"
                     /locus_tag="VFH83_15270"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET6487786.1"
                     /translation="MAKKRDQTDGSGGGQNAPGEGPQPSAADANPQTAGAQQPSETQP
                     SSEPDAPSVAPAVPSPEEQIADLRDKLLRRQADFENFRKRMLREREDAVHYANATLLT
                     DIIGLIDDFERAIKSAEESKDFPNFLQGVTMIEKQFLEVLESRYGLKRFSSVGESFDP
                     NRHEAVQRVEGPAGTRPTVVEDFQKGYFLYDRVLRPARVKVMVPGAEKAPGNDGGEAS
                     AGDEVRAEG"
     gene            complement(5637..>6106)
                     /locus_tag="VFH83_15275"
     CDS             complement(5637..>6106)
                     /locus_tag="VFH83_15275"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="catalyzes the formation of D-fructose 6-phosphate
                     from D-glucose 6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET6487787.1"
                     /translation="LGVTDQHSQVQLYKEGPNDKVYTFLVADRFQKTVPIPPAGPGLD
                     AVSYLGGHTMNELIAAEELATEIALWKAGRPVTRIVFPEISAVSVGEYIHLLEVQTVA
                     AGALFGIDPLDQPGVEEGKQYAYGIMGRPGYEARRREVEEAVQGDADLLFAPK"
BASE COUNT          961 a         2120 c         1952 g         1073 t
ORIGIN      
        1 actccagcaa tcaggttacg gcgaccgtca cgttcaacaa cgagagcgat ggttcctaca
       61 cgttcaaggt gtggtacgac gccgataacg acggcagctc ttatctcgag agcacgtact
      121 acaactatta ctactacaac acctatggct acgtgaacgg ctcgacggtc agcaacgtcg
      181 ggatcaacgg cggcgttacc gccaacgtga cggtaaacct ctacgagtac acgtactagt
      241 ccggctgtct gaacgccagg cgcccccgaa cgggggcgct ttttttgcct cacgctcaca
      301 gggcgtcgtc agcgagcgga aacgtcagac ggaagaggct tccgccgtcc agcgggagat
      361 actccaggaa tccgccgttc gcctgtgcca gacctcgcgc caggtagagc cccagacccg
      421 tgctcgcgga tcgcgaggag ccaacggaga acagatcctg ctccgcgtcg atgggtacgc
      481 cgcctgcccg gtcggagacc tccagcaccg ccgtctctcc ctggcaccgt gcctccacaa
      541 ctacctcgcc ggcatcgtga tgcgggccgg cgtcgcggtc ccggccagcc ccgcggtccc
      601 ggccggcgtc gcggtcccgg ccagccccac ggtaggcttc gatggcgttg cgaagaaggc
      661 acaggaggat ctgctccact tcgcccttgc gggccacgaa ccgaacgccc tccgcaaggt
      721 gcggccgcac caggatcccc tcggcccgaa acggcgtccc cgtctttcgc accaggtccg
      781 cgaagtccct tagggcatcc agggtgcatt cgggctcgcg gccttcccgc gcaagcctca
      841 acaagacgct tacggcagac cgctcgccgg cttcggccaa ctccaggagc agtttcaggc
      901 tctcatcttt agcggccgag tccgcaagcg acagggcgtg gccggccgca agccgcaccg
      961 tcgaaagggt ggagcgaaac tcgtgggccg cggccatggc caggtgggcg gtcgccatga
     1021 gccgaccctg agggcccagc gcctcgtcgg gcctctggag ccgcgcgtgg gcggaccccg
     1081 gcgcggtgcg cagcggataa cgctcctgga acacgagcca cccggtgcac aaagccaggc
     1141 ccccggccag ccaggcgcta cagtccggga cggaccgtcc cacgacccgg gccacggctg
     1201 gcgcggcgac ggccgcgaac cagccagccg ccgcggcgag cgtcaccagg aggtagggcg
     1261 cgcgggtccg cgccgcctgt cgcgccaggc ggaccaggga cgctcctccc aggaacgcca
     1321 gccccaggag atggaacgct cgaaacgaaa gggtgcccgt cagtcccagg gcgaacgaga
     1381 gagcttcggc cgcaaggaac gcgcccccca gggcagccca gccggggcga cgccggctct
     1441 gcggccccga gaggacaagc ccgccccaca ggaccccgag cacgcaaatc gcatacgcca
     1501 ccggattgcc tgcacatgcc acggtacccg cgagcagccc cagaacgccg atcagacaca
     1561 ggggctgcgc ccggcggtcg cacgatggga ggcgcgccgc aacgaggatc gcctggacgg
     1621 aaaggaggga gaggaccgct acgtcgcgag cctggccaac attctgcatg gccaggatgg
     1681 tagcgctact gcttcagctc ggacaaggga accggcttgg ggttcttctc ttccccgttg
     1741 gtgctggcga gctccttgag cagatccttg gcgcgggagc tcagcttggt cgggatcctg
     1801 accaggatct tgacgtacag gtcgccgcga cgggcgggtg catggagctc gggaacgccc
     1861 tcaccccgca ggcgcaggac cttcccattc tgggtgccag cggggacggt gagacgaacc
     1921 gtcttgtcat ccagcgtggg cacgccgatc tccgcaccga gcgccgcctg ggtgacagag
     1981 atagggatgg cgcagtaaag gtcattcccg tctctctcgt agagctcgtg gggcttcacg
     2041 tggatgacga catacaggtc gccatccggt gatccccgag tgcccgcgtc tccctgcccg
     2101 cccaggctca ggcgcttgcc gtcatcgatc cccgccggaa tggtgatggt cagcgtcttg
     2161 cgtccccgga ccgccccggt gccgcggcat tctccgcagg gacgatcgat gatctcccct
     2221 tccccgttgc agtggggaca ggtagaggcg atggaaaaga agccggagtt ccgccggacc
     2281 tgccccgacc cgccgcaggt cgggcacagc cgccggccgc cgcccttgtc ggatcccgtg
     2341 cccccgcaca cgtcacagcg caccgaccgc tcgtaggtca cctcgcgctt ggcgccgaag
     2401 accgcctcgg tgaactccac ctccatgtcg tagcggagat cggatccgcg ctgggacccg
     2461 ccgcgggagg aggatcggcg tcggccggac ccgccgaaga acatgtcgaa caagccgccg
     2521 gaatccccga agatgtcctc gaacccctgg aagatcgttg agaagtcctg ggcgttcggc
     2581 tggcccatcc ctttcacgcc cgcgaagccg tactggtcgt aggcctgtcg cttgccgtcg
     2641 tcggccagga tctcgtaggc ctcggtggct tccttgaact tctcctctgc gtccttgctg
     2701 ccggggttgc ggtccggatg gaacttgatg gcttgttgcc ggtaggcctt tcggatctcg
     2761 tccctcgagg cgcctttggc aacccccagt acctcgtagt aatcgcgttt tgccatgggt
     2821 cagggcttct cgtcgtccac gatctcgtag tctgcgtcat cctgggcggg acgctgcggc
     2881 ttgcggctcc cccgcttacc ggcatggacc cggccgggcg acggcccggg tccatcggaa
     2941 cccgtggaag agccgccgcc gttggagctg ggcccggagg cgtccgttcc gctcgagctg
     3001 cggtccttgt acagctcctc cgccatccgg taggaagccc gccgcaggga gtccatacga
     3061 tctctcacca gggaaacgtc acgggaggtg accgccgcgc gaagctcacg agacgccgcc
     3121 tcaatggcgg cgcggtcctc ggcgctcacg cgggtcccca ggtccctgag cgatctctcc
     3181 gagctgtaca ggagactgtc cgcctcgttg cacacctccg cgcgttccct ctcgacccgg
     3241 tcctcgaccg cgttggcttc cgcctccttc accatccggc ggatctcgtc ttcccgaagc
     3301 ccggaggagc tttcgatccg aaccttctgc tccttgcccg tgccgaggtc tttcgcggaa
     3361 acgtggacga tcccgttggc gtcgatgtcg aaggccacct cgacctgggg cacgccccga
     3421 ggagccggcg gaatgccgac aagatcgaac cgcccgaggg tccggttctg cgaggccatt
     3481 tcccgttctc cctggaggac gtggatcgat accgcggtct gaccgtcact ggcggtagag
     3541 aaggtctggc tcttgcgcgt gggaatcgtg gtgttgcgct cgatgagctt ggtgaacacc
     3601 ccgcccaacg tctcgatccc gagggagaga ggggtgacgt ccaggaggag cacgtccttc
     3661 acgtcgccgc cgaggatgcc tccctggatc gaggcgccga tggcgaccac ctcatccggg
     3721 ttgacgcctt tgcttccctc ccggtcgaag atgtcccgaa cgatggcctg caccgcgggg
     3781 atccgggtgg agcctccgac caggatcacg tggtcgatgt cgcttcgcgt gagacccgcg
     3841 tcccgcagcg cctggaggca tggtccgcgg gtccgctcga acaggtccgc cagaagctgc
     3901 tcgagccgag cccgcgtgag ggtgtaggtg aggtgccggg gtcccgtgcg atccgcggtg
     3961 atgaacggca ggttgatggt ggtggtctgg gcgctggaga gctcgatctt ggccttctcc
     4021 gcggcttcct tcattctctg cagcgccatg cggtccttgg tcagctcgat cccggactcc
     4081 ctcttgaact cggcggccat ccactcgatg atccgctgat cgaagtcgtc tcctcccagg
     4141 tgcgtgtcgc cgttggtgga tctcacctcg aagaccccgt cccccagctc gaggatggag
     4201 atgtcgaagg taccgccgcc gaggtcgtag acggcgatgg tctcctcccg gccgcccttg
     4261 tccaggccat aggccagggc cgccgccgtg ggctcgttca cgatccgctt gacgtccagc
     4321 ccggcgatgc gtcccgcgtc cttggtcgcc tgtcgctgcg cgtcgttgaa gtatgcgggc
     4381 acggtgacca cggcctgggt gacggtctcc cccaggtagt cctccgcggt cttcttcatc
     4441 ttctgcagaa tggcggcgga gatctccggg gcggaatagc gggtgcctct cacctcaacg
     4501 cggacgtcgt tgccgccgtc gacgacccga aaggggacgc gcgcggcctc ctcccctacc
     4561 tcctccatgc ggcgacccat gaatcgcttg atggaataga cggtgctctc cgggttggtg
     4621 atcatctgat tcttggcggg ctggccgacc agatgctccc ccgattccgt gaaagcgacg
     4681 atcgagggag tggtgcgctg tccctcggag ttctggatca cgaccggctg gccgccctcc
     4741 atgaccgcca cgcaggagtt ggtagttccc agatcgatgc ctatgatctt gcccatcgac
     4801 tccctcagcc ttcggcccgg acctcgtccc cggccgaagc ctctcctcca tcgttccccg
     4861 gcgccttttc cgcccccggc accatcacct ttacccgcgc ggggcgaagc accctgtcat
     4921 acagaaaata tcccttctgg aagtcctcga cgaccgtggg tcgagtcccc gcgggccctt
     4981 ccacccgctg gacggcttca tgccggttcg ggtcgaagct ctcccctacc gaggagaacc
     5041 gcttcagccc gtaacggctc tccaggacct cgaggaactg cttctcgatc atggtgacgc
     5101 cctggaggaa gttcgggaag tccttggact cttccgcgga cttgatggct cgctcgaagt
     5161 cgtcgatcag accgatgatg tccgtgagga gcgtcgcgtt ggcatagtga acggcgtcct
     5221 cgcgctcccg cagcatccgc tttcggaagt tctcgaagtc cgcctgccgg cgcagcagct
     5281 tgtccctgag gtcggctatc tgctcctccg gcgaaggcac ggctggagcg acgctgggag
     5341 catccggctc cgaagacggc tgtgtctcgc tgggctgctg ggcgcccgcc gtctggggat
     5401 tggcgtccgc agcggaaggc tgcggtccct ctcctggggc gttctgcccc cctcccgaac
     5461 catcggtctg gtctctcttc ttggccatcg aaatccgcct ggtgtggaaa ttctttgctg
     5521 ctaagataat agcggttggc agcgctggtc aagccactcc gagattcgcc ccggccccgc
     5581 cgcccgtcgg actcctgggc tgcggaccag agggcatacg ggtgcggtgg cggtcgtcat
     5641 ttcggcgcga agagcagatc cgcgtcgccc tgcaccgcct cctcgacctc acgccgcctg
     5701 gcctcgtacc cgggccgacc catgatcccg taggcgtact gcttgccctc ttccacgccc
     5761 ggctggtcca aggggtcgat tccgaacagg gcgccggcgg ccacggtctg gacctcgagg
     5821 aggtggatgt attccccgac actgaccgcc gagatctccg gaaatacgat ccgggtgacc
     5881 ggccgtccgg ccttccagag ggcgatctcc gtagccagct cctccgcggc gatcagctcg
     5941 ttcatggtgt gcccgcccag gtagctgacg gcgtccagcc ccggcccggc tggcggtatg
     6001 ggtaccgtct tctggaaccg gtccgcgacc aggaaggtgt agaccttgtc gttcggccct
     6061 tccttgtaga gctggacctg ggagtgctgg tccgtgacac ccagcg
//
LOCUS       DASRZS010000627         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3820129, whole genome shotgun sequence.
ACCESSION   DASRZS010000627 DASRZS010000000
VERSION     DASRZS010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3820129"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VFH83_15280"
     CDS             <1..560
                     /locus_tag="VFH83_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6487788.1"
                     /translation="VSEGKVLVDGIDVRSWSLESLRSQISVIEQDVFLFSRTIAQNIS
                     FGRPQATRAEIEEAARQAQAHEFVQALPEGYETVIGERGVTLSGGQRQRIAIARAFLT
                     RPRILILDDSTSAIDSATEEEIQKAIRRISREQTTFLITHRLSQIRWADRILVLRRGR
                     LVDQGRHADLLERSMDYRRIFAQYR"
     gene            577..>2266
                     /locus_tag="VFH83_15285"
     CDS             577..>2266
                     /locus_tag="VFH83_15285"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487789.1"
                     /translation="MGFILDGLDSENYDRTYGDRELVRRIIRYFRPYRRQMAVVSVML
                     ALGSLVGTAGPILISKALDVAAARPTLGVLAALSGAVLLLGALGWTFNFVRQRLSARV
                     IGDAVLKLQEDTFSAAIRHDLSFFDAHPSGKIVSRITSDTQDFSNVVSLVMDLANQFL
                     LVVVLTVWLLRINAFLTLLLVAMAPVAVAIALGFRALARRVTQHSRRATATINAQIQE
                     SIRGIVVAKGFRRERAIYETFSDNNARAYQVGVRRGVVLNLIFPILGAAQGLGTAALI
                     YAGAMAMRQGSMTPGTWYLFMQAVGFFWWPLINIASFWSQFQDGLSAAERVFALIDAE
                     PKVTQTGSQSVGRLEGGVELRGIGLRYDTGEPVLRGFDLSVRPRETLALVGHTGAGKT
                     SIARLICRFYEFQEGELLVDGKDIRAFSLDDYRSRVGYVPQDPFLFSTSVLENIRYGK
                     EGASDEEVRRAASHIAGGDWVEDLPAGLATDAGERGANLSMGQRQLVALARVLLKDPA
                     IFILDEATSSVDPFTEAQIQEGLSVLMRDRTAIVIAHRLSTVKHADRILVLDHGQ"
BASE COUNT          360 a          752 c          755 g          399 t
ORIGIN      
        1 acgtgagcga gggcaaggtc ctggtagatg gcatcgatgt tcgctcctgg agcctggagt
       61 cccttcggtc ccagatctcc gtgatcgagc aggatgtgtt cctcttctcc cggaccatcg
      121 cccagaacat ctcctttggc cgaccgcagg cgacccgggc ggagatcgag gaggcggcgc
      181 gccaggccca ggcgcacgag ttcgtccagg cgctgcccga aggatacgag acggtgatcg
      241 gggagcgcgg ggtcacgctc tccggcggtc agcggcaacg gatcgccatt gcccgcgcct
      301 tcctgacccg accgcggatc ctgatcctgg acgacagcac cagcgcgatc gacagcgcca
      361 cggaggagga gatccagaag gccatccgcc ggatcagccg ggagcagacc accttcctga
      421 tcacgcaccg cctgagccag atccgctggg cggaccgcat cctggtgctg cgacggggaa
      481 ggcttgtgga ccagggccgc cacgccgatc tgctggaacg gtccatggac taccgtcgta
      541 tcttcgcgca ataccgctag cgagaagggg ggcagggtgg gattcatcct ggacgggctg
      601 gacagcgaaa actacgaccg gacctacggc gaccgcgagc tggtccgccg gatcatccgc
      661 tacttccgtc cctaccgtcg gcagatggcg gtcgtttccg tcatgctcgc cctgggatcc
      721 ctggtgggga cggcgggtcc catcctgatc tccaaggcgc tcgatgtcgc cgcggcccgc
      781 cccacccttg gggtgctggc ggcgctttca ggggccgtgc tcctcctggg agcgctgggg
      841 tggacgttca acttcgtgcg ccagcggctg tctgcccggg tcatcggaga cgcggtgctc
      901 aagctccagg aggatacgtt ctccgcggcc atccgacacg acctttcctt cttcgacgct
      961 catccttccg gcaagatcgt aagccggatc acgtcggaca cccaggactt ctccaacgtc
     1021 gtgtccctgg tcatggacct ggcaaaccag ttcctgctgg tggtggttct caccgtctgg
     1081 ctcttgcgga tcaacgcgtt cctcacgctc ctcctggtgg caatggcccc ggtcgccgtc
     1141 gccattgccc tgggcttccg cgctctggcc cgccgcgtga cccagcactc gcgtcgcgcc
     1201 acggccacga tcaacgcgca gatccaggag tcgatccgag ggatcgtggt ggccaagggc
     1261 ttccgccggg agcgggcgat ctacgagacg ttctcggaca acaacgcccg ggcgtaccag
     1321 gtcggcgtgc gccgcggggt cgtcctgaac ctcatcttcc cgatcctggg agccgctcag
     1381 ggcctgggca ctgcggcgct catctatgcc ggcgccatgg ccatgcgcca gggcagcatg
     1441 accccgggca cctggtacct gttcatgcag gcggtcggct tcttctggtg gcctctcatc
     1501 aacatcgctt ccttctggag ccagttccag gatgggctct cggccgccga acgggtcttt
     1561 gcgcttatcg acgccgagcc caaggtcacc cagaccggat cccagagtgt cgggcgcctg
     1621 gagggcgggg tcgagcttcg cgggatcggg ctgcgctacg atacggggga gccggtgctt
     1681 cgaggcttcg acctgagcgt ccgtccgcga gagacccttg cgctggtggg gcacaccggc
     1741 gccggcaaga ccagcatcgc ccgtctcatc tgtcggtttt acgagttcca ggagggggag
     1801 ctcctcgtcg acggcaagga catccgtgcc ttctcgctgg acgactatcg gtcccgggtg
     1861 ggttacgtgc cccaggaccc gttcctgttc tctacctcgg tactggagaa catccgctac
     1921 gggaaggagg gggcgagcga tgaggaggtg cggcgagcag cctctcacat cgccggggga
     1981 gactgggtgg aagaccttcc ggcgggcctg gccacggacg cgggtgagcg aggagccaac
     2041 ctctcgatgg ggcaacggca gcttgtggcg ctggcgcgcg tgctcctgaa ggaccccgcc
     2101 atattcatcc tggacgaggc cacctccagc gttgacccgt tcaccgaggc gcagatccag
     2161 gaggggctat cggtgctgat gagggaccga accgccatcg tgatcgccca tcggctctcg
     2221 acggtcaaac acgcggaccg catcctggtg ctggaccatg ggcaga
//
LOCUS       DASRZS010000628         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3053524, whole genome shotgun sequence.
ACCESSION   DASRZS010000628 DASRZS010000000
VERSION     DASRZS010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3053524"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1277)
                     /locus_tag="VFH83_15290"
     CDS             complement(51..1277)
                     /locus_tag="VFH83_15290"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6487790.1"
                     /translation="MKLIAMAWRNLLRQKRRTLVTLSALIVGVCGVVVFQGYIVSLMT
                     AFRESTIRAGIGHLQIAQDPRYFEDGEYNPYAYVGAGGDALAASLSRQPGVIAAFPST
                     GFTAIAGKGDKSVTLLVKGYPSQRMFFPRSGARGSAPADRFTLGELVSGSPLEPGQHD
                     GIVLGETAARILDARPGSVITLMAVLPGGGLNGRDFTVAGVYRSAGQDKIFSFTDYET
                     AVDFTGIRGAPVVHVIADGVGDVDAIAARLPAGLAVRTWHDLATFYVQANTMFNGFLT
                     VIRAIILLVTLFIMANAMNKVVLERMKEWGTLRAIGMKKGRIVLLVVLEGCLQGIVGA
                     SIGIALGFGVAALLNLHGGLPYVRDQSVVIVRLAPSLSSAWRNLIPAALFAGIASFLP
                     GLRAVRLTPSECLRQI"
     gene            complement(1274..1960)
                     /locus_tag="VFH83_15295"
     CDS             complement(1274..1960)
                     /locus_tag="VFH83_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487791.1"
                     /translation="MSLISVRNLSKHYRLEKADAPALVDVSLDIQPGERVCLMGPSGS
                     GKSTLLNLIGCIDTPSEGTVTIDGEDTGAMGDARLSDFRNQRMGFIFQSFNLIPVLSV
                     YENVEYPLLLQKAPARERRARVQELLRAVGLAEYSRRSPENLSGGQRQRVAIARALVV
                     RPRLVIADEPTASLDHATGSSIMQLMVEMNEREGATLVFATHDPSVSSYAKRIVRMLD
                     GRIAEDGRAA"
     gene            2119..2340
                     /locus_tag="VFH83_15300"
     CDS             2119..2340
                     /locus_tag="VFH83_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET6487792.1"
                     /translation="MKESVLKALADIRPALQADGGNVELVDVSDQGVVKVRLTGACSG
                     CPMGMMTLKEGIEKELKSKVPGVKEVIRT"
     gene            2352..>2683
                     /locus_tag="VFH83_15305"
     CDS             2352..>2683
                     /locus_tag="VFH83_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HET6487793.1"
                     /translation="MDKQLEKLIQDQIRNEWYSAYLYLSMAAYFESANLSGFAHWMHK
                     QAGEEQVHGRKMFDFLIDRGVRVALQAIPQPPSEFSSPTDVFEKSYDHEQKVTGLING
                     IADLADKV"
BASE COUNT          498 a          856 c          888 g          441 t
ORIGIN      
        1 tcctcaacgc catgaggatg gttttcaacg gatgcgccat gacaaggctc ctatatctgt
       61 ctcagacatt cggacggggt cagcctcacg gcccgcagac cgggcaggaa ggacgcgatc
      121 cccgcgaaga gggcggctgg aatgaggttc cgccacgccg atgagaggct gggcgccagc
      181 cgcacgatca ccacggactg gtcccgcacg tagggcaggc cgccgtggag gttgaggagc
      241 gccgcgacgc cgaacccgag cgcgatgccg atggacgccc ccacgatgcc ctgaaggcag
      301 ccctccagga cgaccagcag cacgatccga cccttcttca tcccgatggc gcggagcgtc
      361 ccccactcct tcatgcgctc cagcaccacc ttgttcatgg cgttggccat gatgaagagc
      421 gtgacgagca ggatgatcgc gcggatcacc gtgagaaacc cgttgaacat cgtgttcgcc
      481 tggacgtaga aggtggccag gtcgtgccag gtgcgcacgg ccagacctgc cggcaaccgg
      541 gccgcgatgg catccacgtc ccctacgccg tcggctatca cgtgcaccac gggcgcgccg
      601 cggatcccgg tgaagtccac cgccgtctcg tagtcggtga aggagaagat cttgtcctgc
      661 ccggcggacc gatagacgcc tgccaccgtg aagtcccggc cgttgagtcc gccgccgggc
      721 aagaccgcca tcagggtgat caccgatccc ggccgggcat cgaggatgcg cgcggctgtc
      781 tctcccagga cgatcccgtc gtgctgcccg ggctccagcg gggagccgga caccagctcg
      841 cccagcgtga agcgatccgc cggcgcggag ccgcgcgccc ctgaacgcgg gaagaacatg
      901 cgctgcgacg gatacccctt tacgagcaag gtgacggact tgtctccctt cccggcgatg
      961 gcggtgaagc cggtcgaggg gaaggcggcg atgacgcccg gctgccggct gagggaggca
     1021 gccagggcgt cgcctccggc tccgacgtac gcgtacgggt tgtactcgcc gtcctcgaaa
     1081 taccggggat cctgtgcgat ctgcaggtga ccgatgccgg ctcggatcgt gctttcccgg
     1141 aaggcggtca tcagggagac gatgtacccc tggaagacca ccacgccgca gaccccgacg
     1201 atgagcgcgg agagcgtcac cagggtccgc cgcttctggc ggagcaggtt ccgccacgcc
     1261 atggcgatga gcttcatgcc gccctcccgt cttcggcgat gcggccgtcc agcatcctca
     1321 cgatcctctt ggcgtagctg gacaccgacg gatcgtgagt cgcgaacacc agggtggcgc
     1381 cttcccgctc gttcatctcc accatgagct gcatgatcga gctcccggtg gcatgatcca
     1441 gcgacgcggt tggctcatcg gcaatgacca gccgaggccg aaccaccaac gctcgggcta
     1501 tggctacccg ctggcgctgg ccgccggaga ggttctccgg gctgcggcgg ctgtactcgg
     1561 caagacccac ggcacgcagg agctcctgta cgcgggctcg tctctcccgc gccggcgcct
     1621 tttgcagcaa caggggatac tcgacgttct cgtacacgga gagcaccggg atcaggttga
     1681 agctctggaa gatgaacccc atccgctggt ttcggaaatc ggagaggcgg gcgtctccca
     1741 tggctccggt gtcctcgccg tcgatggtga ccgttccctc ggagggggtg tcgatgcagc
     1801 cgatcaggtt gaggagggtg gacttcccgc ttcccgacgg gcccatcagg cacacccgct
     1861 ctcccggctg gatgtccagg ctcacgtcga cgagagccgg ggcatcggcc ttctccaggc
     1921 ggtagtgctt gctcaggtta cgaacgctta tcagggacat gaacgtctcc tttcgtttca
     1981 ggttccagag ccaatatgcc atccggcgcc ggcaggctgg cagtgccgaa agtcacggaa
     2041 aggtcatagt gacggggtaa cctttactcg gggggcggtt tcaggttacc cttgccgcac
     2101 ccggaggaga ggaccatgtt gaaggaatcg gtgctcaagg cgctcgccga catcagacct
     2161 gccttgcagg cggacggcgg caacgtggag ctggtggacg tgtcggatca gggcgtcgtg
     2221 aaggtgcggc tgacgggcgc ctgcagcgga tgccccatgg gcatgatgac gctgaaggaa
     2281 gggatcgaga aggagctcaa gtcgaaagtg cccggggtaa aagaggtcat ccggacctga
     2341 gaggaatcga tatggacaag caacttgaga agctgatcca ggatcagatc cgaaacgagt
     2401 ggtactcggc gtacctgtac ctgtccatgg ccgcgtactt cgagtcggcc aacctctcgg
     2461 gcttcgcgca ctggatgcac aagcaggccg gagaggagca ggtccatggc cgcaagatgt
     2521 tcgacttcct gatcgatcgc ggcgttcgcg ttgcgctcca ggccatcccg cagcctccgt
     2581 cggagttcag ctcccccacg gacgtgttcg agaagagcta cgaccacgag cagaaggtca
     2641 cggggctcat caacgggatc gcggacctcg cggacaaggt aaa
//
LOCUS       DASRZS010000629         3101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1520899, whole genome shotgun sequence.
ACCESSION   DASRZS010000629 DASRZS010000000
VERSION     DASRZS010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520899"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFH83_15310"
     CDS             complement(<1..430)
                     /locus_tag="VFH83_15310"
                     /inference="COORDINATES: protein motif:HMM:NF013663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET6487794.1"
                     /translation="MADKRREIRKVLTVVNSQKSDVGSVVRVIEEFARARGIAVASLD
                     YSDHPPQEALADVDLAISLGGDGTLLSCARMLAPRGIPILAVNIGDFGFITEVSRHEL
                     VETWEKLLAGQLGVSERLMLSVTVQREGAPVASFVGLNETV"
     gene            complement(499..1524)
                     /locus_tag="VFH83_15315"
     CDS             complement(499..1524)
                     /locus_tag="VFH83_15315"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6487795.1"
                     /translation="MAVPLFRPTVKRRDMGSVLSCIVSDKLGPGEISRELVSRAVHLL
                     GQSGGVSLPNTYLALAVALRALGLSSGDGVVLPALAPSLWLRVVQELGLVARVADVDP
                     QSGLIDVVEAARLTQDGAKAILVPHTLGMPVDLQALRGLGVPILEDISQGLGGKMGDQ
                     MTGAGGDLCLLSLEPECIVTCGGGALVLAKGRQMAAALRRVTESSPLYSQLADMNAAL
                     GISQFAALDSFIAVRREVATAYAQSLLKSRHKGLAQTAEAEVVLSSFPVLLNDGMKEV
                     RQYALKRNVETAPAFADTVASLEGTDAAHCVNARSLLLRCLLFPLYPTLGKRDVETTC
                     KVLATLP"
     gene            complement(1648..2310)
                     /locus_tag="VFH83_15320"
     CDS             complement(1648..2310)
                     /locus_tag="VFH83_15320"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET6487796.1"
                     /translation="MPTEHPLIASAERMVRLQLRTRDIVDDRVLDAMSRVPRESFVPT
                     GSRAKAYDDSPLPIGHRQTISQPYMVAFMTQALALRGPERVLEVGTGSGYQTAVLAEL
                     CRQVFSVERIPELAATALRAVADLGYCNVTIRVGDGSAGWPEEAPFDRILVGAAAPAV
                     PPVLGDQLADNGILVVPVGDWRGTQVVVLVRRTGRVLSVEQTIGCRFVPLIGTGGFPP
                     AG"
     gene            complement(2325..>3101)
                     /locus_tag="VFH83_15325"
     CDS             complement(2325..>3101)
                     /locus_tag="VFH83_15325"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6487797.1"
                     /translation="VKAEVKVVFVVDSRKTDLPIIYTASHFDYGFERVYIPPEPGLKG
                     FYDKVRTDIRAFGENCLAKCRKDAESVGVPLLAVLREGLPSGILGEEARSGDLLVLGQ
                     KGENAHIQRAIVGSTTEDVVRSSPRPVLICPAAFKSPVRVLFPYDGSPVAESALQFCV
                     NAFGSIWQEIVVLTVTEEMEESFPYDTELGYLGTHGIPYRLLIEAGKPVDTIPRIAKR
                     EGADIILVGAHGRHKIREYLLGSTASHLIRRSDVPVIVAF"
BASE COUNT          517 a         1076 c         1016 g          492 t
ORIGIN      
        1 tgaccgtctc gttgagcccg acgaaggatg ctaccggcgc gccctctcgc tggaccgtga
       61 ccgagagcat caggcgttcg ctcaccccca actggccggc gagcagtttc tcccacgtct
      121 ccaccagctc gtgtcgcgat acctccgtta tgaacccgaa gtccccgatg ttcaccgcca
      181 ggatggggat tccgcggggt gccagcatgc gggcgcagga gaggagcgtc ccgtcccctc
      241 ccagggagat ggcgaggtcc acgtccgcga gggcttcctg aggcgggtgg tcggagtaat
      301 ccagcgaggc taccgctatc ccgcgggcgc gggcgaattc ttctatgacg cgcacaacgc
      361 tgccgacatc gctcttctgc gagttgacaa cggttaatac cttgcggatt tccctccgct
      421 tgtctgccat gtgcgcctac tatagttgcc cgccggcgcg aaggcaagcc gcacccctct
      481 cccggggcgc gccacccgct agggcagggt tgccagcacc ttgcaggtgg tctccacgtc
      541 ccgctttccc agggtgggat acagggggaa gaggagacag cgcaggagaa gcgatcgggc
      601 gttcacgcag tgggcggcgt cggtgccctc cagagacgcc acggtatccg cgaaggcggg
      661 cgcggtctcg acgttccgtt tgagggcata ctggcggacc tccttcatcc cgtcgttcag
      721 cagcacgggg aaggatgaca ggaccacttc cgcctccgcg gtctgggcga gccccttgtg
      781 gcgggacttc agcagggact gggcgtacgc cgtcgcgacc tcccggcgga cggcgatgaa
      841 gctgtccagc gccgcgaact gagagattcc gagggcggcg ttcatatcgg cgagctggga
      901 gtacagcggc gagctttccg tcacccggcg gagggccgcg gccatctggc gccccttcgc
      961 gaggaccaac gcccccccgc cgcacgtgac aatacactcc ggctcgaggg acagcaggca
     1021 gagatcgccg ccggccccgg tcatctggtc gcccatcttg ccgcccagcc cttgggagat
     1081 atcttccagg atgggcacgc cgagcccccg cagcgcctgg aggtccaccg gcatccccag
     1141 ggtgtgcgga acgaggatgg ccttggctcc gtcctgggtc agtcgggcgg cctcgacaac
     1201 gtcgatcaat ccgctctgag ggtccacgtc agccacccgc gcgaccagac cgagctcctg
     1261 gacgacccgc agccagagcg agggggcgag cgccgggagg accaccccgt ccccggagct
     1321 caaccccagg gcccgcagcg ccaccgcgag cgccaggtag gtattgggga gggacacgcc
     1381 ccccgactgc ccgagaaggt gcaccgcgcg ggacacgagc tcacgcgata tctcgccggg
     1441 acccagcttg tccgagacga tgcaggacag gacgctcccc atgtcccttc gcttcaccgt
     1501 ggggcggaac aacggaactg ccatttctct gacttccttt tggggctctg cgcgcggggt
     1561 gcgcagcctg ggctgcattg ggctccgaag gccgtggttt gtcaataacg ccggaggttc
     1621 ccgcggcctg gggcgcgatc cgggagatca gcctgccggc ggaaacccac ccgtgccgat
     1681 cagaggaacg aaccggcagc cgatggtctg ttcgacgctg agcacccgtc cggtcctccg
     1741 caccagcacc acgacctgcg tcccccgcca gtctccgacc gggaccacga ggatcccgtt
     1801 gtccgcgagc tggtctccaa ggaccggggg aacggcgggg gccgccgcgc cgaccaggat
     1861 ccggtcgaat ggcgcctcct ccggccaacc cgcgctcccg tctccgacgc ggatggtgac
     1921 gttgcagtac cccaggtccg caacggcgcg cagcgcggtg gccgccagct cggggattcg
     1981 ctccaccgag aagacctgtc gacagagctc cgcaagcacg gcggtctggt agccgctgcc
     2041 cgtgcccacc tcgagcaccc gctcgggtcc ccgcagcgcc agcgcctgcg tcatgaaggc
     2101 caccatgtag ggctgggaga tcgtctgacg atgaccgatg gggagaggac tgtcgtcgta
     2161 agctttggcc cgggaccccg tgggaacgaa ggactcccgc ggcacccggc tcatggcgtc
     2221 cagcacccgg tcatcgacga tgtcccgagt ccggagctga agccggacca tccgttccgc
     2281 tgaggcaatg agaggatgct ccgtgggcac ggcaggcctc tgggctagaa ggcgacgatg
     2341 accggtacgt cgctcctgcg gatcaagtga gaggcggtgg atcccaggag gtactcccgg
     2401 atcttgtgcc ggccatgagc ccccaccagg atgatgtccg ccccctcccg cttggcgatt
     2461 cgagggatgg tatccaccgg ctttcccgcc tcgatcagga ggcgataggg gatcccgtgg
     2521 gtccccaggt agcccagctc cgtgtcgtat gggaagctct cttccatctc ctcggtcacc
     2581 gtgaggacga cgatctcctg ccagatgctc ccgaacgcgt tcacgcagaa ctggagggcg
     2641 ctctccgcga ccggacttcc atcatacggg aacaggacgc ggaccgggct cttgaatgcc
     2701 gccggacaga tcagcacggg ccgggggctg gagcgaacca catcctccgt ggtggacccc
     2761 acgatggcgc gctggatgtg cgcgttctcg cccttctggc cgagcaccag caggtctccc
     2821 gagcgcgctt cctcccccag gatgcccgag ggaagcccct ccctcaggac cgccagcagc
     2881 gggaccccga cgctctccgc gtccttgcgg cacttggcca agcagttctc tccgaaggcg
     2941 cggatgtccg tccgcacctt gtcgtagaac cccttgaggc ccggctccgg agggatgtag
     3001 acccgctcga atccgtaatc gaaatggctt gccgtgtaga tgatcggcag gtcggtcttg
     3061 cgggagtcga cgacgaaaac gaccttcacc tcggccttca c
//
LOCUS       DASRZS010000630         6187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5736207, whole genome shotgun sequence.
ACCESSION   DASRZS010000630 DASRZS010000000
VERSION     DASRZS010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5736207"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VFH83_15330"
     CDS             <1..774
                     /locus_tag="VFH83_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6487798.1"
                     /translation="EVVGKALRGRREKVILATKCSLTWAPGERAIRHNLKAVSVRAEI
                     EASLRRLATDYIDLYQIHWPNPEPEIEEGWGEIARAVKAGKIRFAGVSNFSVAQMKRI
                     AKIHPIASLQPPYSMLRRDVEPEILPFCKANGIGVICYSPMQMGILSGRFTRERAAAL
                     PGNDQRSKRADYTEPVLSANLDLVEKLRPIAAGRSVGELAIAWVLRRPEVTAAIVGAR
                     RPSQIEELATASEWELSPADVRRIDSLLSEREKRIKPSA"
     gene            complement(789..1304)
                     /locus_tag="VFH83_15335"
     CDS             complement(789..1304)
                     /locus_tag="VFH83_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6487799.1"
                     /translation="MERHLCVALLAKIDEQIERTDHLAAQVPEGAVDWTPPTPRDFSV
                     GVLLGHLLECLAGFCAVLYAARPQALGHFQDLRRLPVNHRCAPPEARERMAVYRARIG
                     EGFALLEDGDLGKAVPTVFVRDGELLLTLLLGNLEHLVNHKHQLFSYLKLMGVEIGSR
                     DLYRFRGNHAS"
     gene            complement(1307..2665)
                     /locus_tag="VFH83_15340"
     CDS             complement(1307..2665)
                     /locus_tag="VFH83_15340"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET6487800.1"
                     /translation="MSGSAEYAELFRPQFHFTARRNWINDPNGLVFYEGEYHLFFQRH
                     ASGITWGPMSWGHAISRDLVHWEQIDDALQPDDRLGYIWSGSAVVDWQNTSGLGTGED
                     PALIACYTSIDPAPQSGKPGVQCIAYSRDRGRSWTKYPGNPVLPHVDGANRDPKILWH
                     PSTRGWVMALYLDRSDYALFGSPDLRAWNRLDSVTIPGTAECPDFFELPVDGDPGQRS
                     WVFWGATGGYLLGSFDGRHFAPQTDPLSAEMGPNGYAAQTWSDIPASDGRRIQISWMR
                     NGRYPYMPFNQQMSFPVELTLRRFPEGVRLCRLPVREIETLWKDRETWDSRPLAAGQP
                     FALKTKGDLFDMQLVVELGASVESLSLRIYGNELRYLPARATATYLGKEIAIDAPDRR
                     LELRLLVDRASLELFSPRGRVSASFCFLPEAWDAPIELMAQGGAARIASLEIRELSSI
                     WR"
     gene            complement(2662..2919)
                     /locus_tag="VFH83_15345"
     CDS             complement(2662..2919)
                     /locus_tag="VFH83_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HET6487801.1"
                     /translation="MRIKLSHEVEQRLVASIKRYYREEGGEEIGDLKARLLLDFCVRE
                     VGPCIYNQAIADAQARMLSKVADLEGECFEPEFSYWKGRKE"
     gene            3052..4293
                     /locus_tag="VFH83_15350"
     CDS             3052..4293
                     /locus_tag="VFH83_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015843435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6487802.1"
                     /translation="MRQLTQGINLEDVQDMNRALVIRILRKLRLCSRADLSKATGLTQ
                     PTITNIIGDFIKWHLVTEKGIIEGEKGRRSIGISLNTELYKVIGVRLARKYFSVGMFD
                     LFGSGEILTQEAIDVEEGSVAALQRIRAAVKNLIASSSQFRILGVGVAIPGPFFRTEG
                     RIAIMTEFPGWEKVDLEEELGASFGVPVFLEHDANAGALAEWWLGPHSRETGTMVYVA
                     AGQGVGAGIMIDGRMFRGTLGTAGEIGHMSISYDGPPCECGSRGCLEHYCSTIALMRE
                     TQRNLDRFPSSALGKGHSFPAIVKALQDGDELAREQVEKAAWFLGFGLVNVVNTFNPD
                     VIIIGDDLAQTGQPLLDVVVRTVKQHVLPSVFANLRIELSSFEIDPVLVGVSTLVVEK
                     LLATPSAIQQIGVTFQAARGN"
     gene            complement(4303..5661)
                     /locus_tag="VFH83_15355"
     CDS             complement(4303..5661)
                     /locus_tag="VFH83_15355"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET6487803.1"
                     /translation="MTLDLPYHELHRPQFHFSPRTNWTNDPNGLVYYQGEYHLFYQLN
                     AKGIRWGPNTWGHAVSPDLVHWQQLGHAIEPDEYGWIWSGSAVVDHGDTTGFKDGPED
                     PLVAVYSTGGVGRFGFPKNDVVQCIAFSNDRGRTWTKFAGNPVLPHVRAENRDPKVIW
                     HEPTGQWVMALYLDASDFALFGSRDLKSWSRLCDVPLPGTGECPDFFPLAVDGDPGRV
                     RWVFWGGGGLYRIGSFDGKTFRPETEPLKAELGANGYAAQTWSDVPKADGRRIQVSWM
                     AGGKYPSMPFNQQMTFPVTLSLRSFPEGDRLCRNPVREIAGLNDAPHEWRNETVTPDR
                     RLVPPTRHDLFHITGSAVLHGASSWGLVLRGIELRYHAAERKFTYLGKAVPCDPGSEP
                     LDFEVLLDRTSMELFACGGRVSASFCFLPEAWDDPVEVFADGGRVSFPSLCVRELSSA
                     WR"
     gene            complement(5726..>6187)
                     /locus_tag="VFH83_15360"
     CDS             complement(5726..>6187)
                     /locus_tag="VFH83_15360"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HET6487804.1"
                     /translation="ARCKEWLVSILGPETALRVEHYGSTALEGIPAKPIIDILVEIPE
                     LDLAMPSIVPRLNDPFWEYWWYKDHLTFIRRDRLGGTRTHHLHMAPRGHAVWQGVAFR
                     DYLRSHPRDAARYADLKRRLAGDFRDDRERYTDRKEAFVREIAATAATGAG"
BASE COUNT         1125 a         2000 c         1995 g         1067 t
ORIGIN      
        1 gaggtcgttg ggaaggcgct tcgcggccgc cgggagaaag tcattcttgc gaccaagtgc
       61 agcctgacct gggcacccgg cgagcgggcc atccggcaca acctgaaggc ggtgagcgtc
      121 cgagccgaga tcgaggccag cctgcgaagg ctcgccacgg actacatcga cctctaccag
      181 atccactggc ccaacccgga gcccgagatc gaggagggat ggggcgagat cgcgcgggcc
      241 gtgaaggccg gcaagatccg cttcgccggc gtctccaact tcagcgttgc gcagatgaaa
      301 cgcatcgcga agatccatcc cattgcttcc ctgcagccgc cttacagcat gctgcggcgg
      361 gacgtggagc cggaaatcct gccgttctgc aaggcaaacg gcatcggggt gatctgctat
      421 agccccatgc agatgggcat cctctccggg cggttcacgc gggagcgagc cgcagcgctt
      481 cccggaaacg accagcgttc caaaagggcc gactacaccg agcctgtgct gagcgcaaac
      541 ctggacctcg tcgagaagct caggcccata gccgccggcc ggtcggtagg agagctggcc
      601 atcgcgtggg tgctccgcag gcccgaggtg acggccgcca tcgtgggcgc gcgtcggccg
      661 tcccagatcg aggagctggc aaccgcatcg gaatgggagc tcagccccgc ggatgttcgg
      721 cgcatcgact ccctcctgtc cgagcgtgag aagcggatca agccatccgc ctgaggaccc
      781 ttcgatcgct acgacgcgtg gtttcctcga aaccggtaga ggtcccggct gccgatctct
      841 acgcccatca gcttgaggta gctgaagagc tgatgcttgt ggttgaccag gtgctccagg
      901 ttgcccagga gcagggtcag cagcagctcc ccgtcgcgaa cgaacacggt cggaaccgcc
      961 ttgcccaggt cgccgtcctc cagaagcgcg aacccctcgc cgattcgggc gcggtagacg
     1021 gccatccgtt cccttgcttc gggcggcgcg catcggtgat tcaccggcag tcggcgcaga
     1081 tcctggaagt ggccaagagc ctgcgggcgc gccgcgtaga gtacggcgca gaatcccgcc
     1141 aggcactcca ggagatgtcc cagcaagacc ccgacggaga agtcgcgggg ggtcggcggc
     1201 gtccagtcga cggcgccttc cggcacctgg gccgccagat gatcggtccg ctcgatctgc
     1261 tcatcgatct tggccagcaa ggccacgcac agatgccgtt ccatccctac ctccagatgg
     1321 aggagagctc gcggatctcc agggatgcga tgcgggccgc tccgccctgt gccatgagct
     1381 cgatcggcgc atcccacgct tcgggcagga aacagaacga cgcggaaacc ctgcctctgg
     1441 gggagaaaag ctccagggaa gcgcggtcca ccaggagacg aagctccagg cggcgatccg
     1501 gagcatcgat ggcgatctct tttccgaggt acgttgccgt tgctcgcgcc ggcagatagc
     1561 gcagctcgtt cccgtagatc cggagggaca gactctctac gctcgccccg agctcgacga
     1621 cgagctgcat gtcgaaaagg tcccctttgg tcttcagcgc gaacggctgc cccgcggcga
     1681 gcggccgtga atcccaggtc tctcggtcct tccacagggt ctcgatctct ctcacgggca
     1741 ggcggcagag ccgaaccccc tcgggaaacc tgcgaagggt gagctcgacg gggaaagaca
     1801 tctgctgatt gaacggcatg tacggatacc gtccgttccg catccaggag atctggatcc
     1861 gccgtccgtc ggatgcgggg atgtcgctcc aggtctgggc agcgtacccg ttggggccca
     1921 tctccgcgga gagcgggtcc gtctgcgggg cgaaatgcct gccgtcgaac gaacccagaa
     1981 ggtacccgcc cgtggcgccc cagaagaccc acgaacgctg gccgggatcc ccatccacgg
     2041 gaagctcgaa gaagtctggg cactccgcgg tgccgggaat cgtcaccgaa tccagtcggt
     2101 tccaggcgcg gaggtcgggg gagccgaaca gcgcatagtc gcttcggtcc aggtagagcg
     2161 ccatgaccca gccacgggtg gacggatgcc ataggatctt cgggtcccgg ttggcgccat
     2221 cgacatgagg caggaccggg tttccggggt actttgtcca gctgcggccc cggtccctgc
     2281 tgtaggcgat gcactgcacg cccggtttgc cggactgcgg agccggatcg atgctcgtgt
     2341 agcaggcaat cagggccgga tcctctcccg tcccgaggcc ggaggtgttc tgccagtcaa
     2401 cgaccgcgga cccggaccag atgtagccca ggcggtcgtc cggctggaga gcgtcgtcga
     2461 tctgttccca gtgcaccagg tcccggctga tcgcatgtcc ccagctcatc ggaccccagg
     2521 tgatccccga tgcatgccgc tggaagaaca ggtggtactc cccctcgtag aacaccaggc
     2581 cgttgggatc gttgatccag ttcctccgcg ccgtgaagtg gaactgcgga cgaaacagct
     2641 cggcatactc cgcgctcccg ctcattcctt cctccctttc cagtagctga actccggctc
     2701 gaagcattcc ccttccagat cggcgacctt gctcagcatg cgggcctggg cgtccgcaat
     2761 cgcctggttg tagatgcatg gcccgacctc tcgaacgcag aagtccagga gaagcctggc
     2821 cttgaggtct ccaatctcct ccccgccttc ctcgcggtag tagcgcttga tcgacgccac
     2881 caagcgctgt tcaacctcat gggatagctt tatgcgcatg tgttcccctc ggcggtaggg
     2941 atctcgctcg aaacggccgc cagctttctt gacaactgga gatcagttcg taatactgtg
     3001 tcaagagttc attcaattta tggatttttg catttatcac agggcaacgc gatgagacaa
     3061 ctcacccaag gaataaacct ggaagatgtg caggacatga atcgcgccct ggtgatccgg
     3121 atcctgcgca agctcaggct ctgctcccga gccgatcttt ccaaggcgac cggccttacc
     3181 caaccgacca tcaccaacat catcggtgat ttcatcaagt ggcacctggt cacggagaag
     3241 ggaatcatcg aaggggaaaa ggggcgtcgt tccataggga tttccttgaa caccgagttg
     3301 tacaaggtca tcggtgtgcg gcttgcgcgc aagtacttct ccgtcgggat gttcgacctg
     3361 ttcggttccg gcgagatcct cacgcaggag gccatcgacg tcgaggaagg ctccgtcgct
     3421 gccctccagc gaattcgcgc cgcggtgaag aacctcatcg cctcctcgtc ccagttccgt
     3481 atcctggggg tcggggtcgc catcccgggg ccgttcttcc gcaccgaggg ccggatcgcg
     3541 atcatgaccg agttcccggg ctgggagaag gtagacctcg aggaggagct cggcgcctcc
     3601 tttggggtgc cggtgttcct ggagcacgac gcgaacgccg gggcgctcgc cgaatggtgg
     3661 ctggggcctc atagccgtga gaccggcacc atggtgtacg tggccgcggg tcagggcgtc
     3721 ggggccggca tcatgatcga cggccggatg ttccgaggga ctctgggcac cgccggtgag
     3781 attggccaca tgagcatctc ctacgacggg cctccgtgcg aatgcggaag ccgcggctgt
     3841 ctcgagcact actgctccac gattgccctg atgcgggaga cccagcgcaa cctcgatcgc
     3901 ttcccgagct ccgcgctcgg caaggggcac tcctttccgg cgatcgtgaa ggcgcttcag
     3961 gacggcgacg agctggcgcg ggagcaggtg gagaaggccg cgtggttcct gggcttcggc
     4021 ctggtgaacg tcgtgaacac gttcaacccg gacgtgatca tcatcgggga cgacctggcc
     4081 cagaccggac agcccctcct ggacgtcgtg gtgcgcaccg tgaagcagca cgtcctcccc
     4141 agcgtgttcg ccaatctgcg catcgagctg agctctttcg agatcgatcc ggtgctggtc
     4201 ggcgtgagca cgctggtggt ggagaagctc cttgccaccc cttccgccat ccagcagatc
     4261 ggcgtgacct tccaggcggc tcggggcaac tgagccgggc ctctacctcc aggcgctgga
     4321 aagctcccga acgcagagag acgggaagct cacccggccg ccgtcggcga acacctccac
     4381 gggatcatcc caggcctccg gcaggaagca gaaggacgcg gagacccttc ccccgcaggc
     4441 aaagagctcc atggacgtcc gatccagcaa cacctcgaag tccagaggtt cggatccagg
     4501 atcacagggc acggccttgc cgaggtaggt gaacttcctc tccgcggcat ggtagcgaag
     4561 ctcgatcccg cgaagcacca gtccccagga ggaggctccg tgcaacacgg cggagccggt
     4621 gatgtgaaag agatcatgac gggtaggggg gacgagcctg cggtccggcg tgacggtctc
     4681 gttgcgccac tcgtgcggcg cgtcgttcag accggcgatt tcccggaccg gattgcggca
     4741 gaggcgatcg ccttccggga acgatcggag agacagcgtc accgggaaag tcatctgctg
     4801 attgaagggc atggaggggt actttcctcc ggccatccag gagacctgga tcctgcgtcc
     4861 atccgccttg gggacgtcgc tccaggtctg ggccgcgtag ccgtttgcgc cgagctccgc
     4921 tttcagcggc tccgtctccg gacggaaggt cttgccgtca aagcttccga tccgatatag
     4981 acccccgccg ccccagaaga cccaccgcac ccggccgggg tctccgtcca ccgcaagcgg
     5041 aaagaagtcc gggcattcac ccgtccccgg aagcgggaca tcgcacagcc tggaccagct
     5101 cttgagatcc cgggacccga acagggcgaa atcgctcgcg tccagataca acgccatgac
     5161 ccactgcccg gtcggctcgt gccagatcac cttaggatcc cggttctccg cccgcacgtg
     5221 gggcagcacg gggttgccgg cgaacttggt ccaggttcgg ccccggtcgt tgctgaacgc
     5281 gatgcactgc acgacgtcgt ttttggggaa gccgaatcgg ccgaccccgc ccgtagagta
     5341 gacagccacc agggggtcct cgggaccatc cttgaacccc gtggtgtcgc catgatccac
     5401 caccgcggag ccggaccaga tccaaccgta ttcgtccggt tcgatggcgt ggccgagctg
     5461 ctgccagtgc accaggtccg gactgacggc gtgcccccac gtgttgggtc cccaccggat
     5521 gcccttggcg ttgagctggt agaagagatg atactcgcct tggtagtaga ccagcccgtt
     5581 cggatcgttg gtccagttgg tgcgagggga aaagtggaac tggggccggt gaagctcgtg
     5641 gtacggcagg tccagggtca cgggcgcctc ctcaatcgcg tgatggctgc aaacggcgcc
     5701 actatcgtcg cgccgagcct gcctgtcaac cggcgcccgt tgccgccgtt gcagcaatct
     5761 ctcggacgaa cgcctccttc cgatccgtgt accgttcgcg gtcgtcgcga aagtcaccgg
     5821 ccagccgacg cttgagatca gcgtatcgcg ccgcatcccg gggatgggaa cggagatagt
     5881 cacggaacgc gacgccctgc cacaccgcgt gaccccgcgg cgccatgtgg agatggtggg
     5941 tgcgcgttcc gcccagccga tcgcgacgga tgaacgtgag gtggtccttg taccaccagt
     6001 attcccagaa cggatcgttg agacggggga cgatggaggg catggccagg tcgagttccg
     6061 gtatctctac aaggatgtcg atgatgggct tggcggggat gccctccagg gcagtgctgc
     6121 cgtagtgctc cacccgaagc gccgtctcgg gacccaggat ggacaccagc cactctttgc
     6181 agcgggc
//
LOCUS       DASRZS010000631         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_731597, whole genome shotgun sequence.
ACCESSION   DASRZS010000631 DASRZS010000000
VERSION     DASRZS010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731597"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VFH83_15365"
     CDS             complement(<1..454)
                     /locus_tag="VFH83_15365"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET6487805.1"
                     /translation="MRGRPMAEAGHNAEEPFEVRDYECDLQGVVNNAVYLHYLEHARH
                     RWLEAIGVDFAALHQQGMDLVVARIEVDYRSPLRSRDRFVVRSRIQREGRLKLIFQQE
                     IFRVPDEKLVIQARVVTAALQNGRPVVAQAVLDRLEGTSAGTGDAPPGS"
     gene            complement(451..918)
                     /locus_tag="VFH83_15370"
     CDS             complement(451..918)
                     /locus_tag="VFH83_15370"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HET6487806.1"
                     /translation="MERPVTIVDMTARKLIGLRSRATAQTVSKSMLATLTRVWEVIRK
                     DNLKTLGLNVAIYYTPEQGAPSPSDFGFVAGVLVDGDIGQAAGLEVLETPTGAFAHAE
                     LMGDYGLIPEVHRQIRDWCQANGKRLAGVNLEIYGHWTGDQSQARTDVYYLLG"
     gene            complement(950..1867)
                     /locus_tag="VFH83_15375"
     CDS             complement(950..1867)
                     /locus_tag="VFH83_15375"
                     /inference="COORDINATES: protein motif:HMM:NF024875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HET6487807.1"
                     /translation="MIDEVQRECSTHGDRAVVVSRDLLETVRRDLAELSERETLGGFQ
                     RHILGSIYVTEAPRTGFPVASILIVATTTPAFARLQFTRNGRRTEVMQPAGYIDKEAA
                     PHRIQRYLSGLLDSGGLHLLPEPRLPRKLLAARSGLAAYGRNNICYVDGMGSFCTLST
                     FFSDLPCAASPAKELRMMDTCSGCRECQRRCPTGAIRGDRFLVDAERCLTFYNEAGGF
                     PFPDWIPQDAHNSLYGCHRCQTACPVNQDRLGHGLEPTAFDEEETAMLLEGRPAERLP
                     PNLSQKVASLNMTEYLPALPRNLRVLGIG"
     gene            complement(1916..>2360)
                     /locus_tag="VFH83_15380"
     CDS             complement(1916..>2360)
                     /locus_tag="VFH83_15380"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6487808.1"
                     /translation="VARQRLGGRAGVTILPPVDAGGTSIGNGRPADVVTAIQSLHYQD
                     PESRKASIANCHRLLGDGGLFVTFENIRPLTARGTEIGKSRWRRFQERSGRSPAEAER
                     HLARFDVEYFPITAEEYLGVLKDVGFRTVEILWYSYMQAGFYGIR"
BASE COUNT          382 a          813 c          743 g          422 t
ORIGIN      
        1 cggaacccgg cggcgcgtcc ccggtcccgg cgctcgtgcc ttccagccga tccagaacgg
       61 cttgggccac caccggtcga ccgttctgca gcgccgcggt gacgactcgg gcctggatca
      121 cgagcttctc gtctggcacg cggaagatct cctgctggaa gatcagcttg agcctgcctt
      181 cccgttgaat ccgggaccgg accacgaacc gatcccggct gcgcagcggg gagcggtaat
      241 ccacctcgat ccgcgccacg acgaggtcca tgccctgctg gtggagcgcg gcgaagtcga
      301 cgcctatcgc ctccagccag cgatggcggg catgctcgag gtagtgcagg tagaccgcgt
      361 tgttcaccac gccctgaaga tcgcactcgt aatcgcggac ctcgaagggc tcctcagcgt
      421 tatggccggc ttccgccatc ggtcgccctc tcatcccagg agatagtaga catccgtcct
      481 ggcctggctc tggtcaccgg tccagtggcc gtagatctcc aggttcacgc cggccagcct
      541 tttcccgttg gcctggcacc aatcccgaat ctgccggtgg acctcgggga tgagcccgta
      601 gtcccccatc agctcggcgt gagcgaaagc cccggtgggc gtttccagga cctccaggcc
      661 cgccgcctga ccgatgtctc catccaccag gacgccggcg acgaagccga agtccgatgg
      721 agacggcgcg ccctgttccg gggtgtagta gatggcgacg ttcagcccga gggtcttgag
      781 gttgtccttt cggatcacct cccagaccct ggtcagcgtt gccagcatcg acttcgacac
      841 ggtctgcgcg gtggccctgg accgaaggcc tatcagcttg cgagcggtca tgtccacgat
      901 cgtaacgggt cgctccaatc ctatgctcct tcccatctcc aatgcttcct cagccgatcc
      961 cgagcactct gaggttccgc ggcagggccg gaaggtactc ggtcatgttc aacgaggcca
     1021 ccttctggga gaggttcggg gggagccgtt ccgcgggcct cccctccagg agcatcgcgg
     1081 tctcctcctc gtcgaacgcc gtcggctcca ggccatgccc caggcgatcc tggttgaccg
     1141 gacaggctgt ctgacaccgg tggcatccgt acagggagtt gtgcgcatcc tgcggaatcc
     1201 agtccgggaa tgggaagccg ccggcctcgt tgtagaacgt caggcaacgc tcggcatcga
     1261 ccaggaagcg gtctccgcgg attgcgccgg tggggcacct gcgctggcat tctctgcagc
     1321 cggaacacgt gtccatcatc cgcagctctt tcgccggcga ggccgcgcac ggcaggtcgg
     1381 agaaaaaggt cgagagcgtg cagaagctgc ccatgccgtc gacgtagcag atgttgtttc
     1441 ggccgtaggc cgccaggccg cttcgcgcgg cgaggagctt ccgaggcagc ctgggctccg
     1501 ggagaaggtg caagccgccg gaatccagga ggccggagag gtaacgctgg atgcggtggg
     1561 gggcggcctc tttgtcgatg taccccgccg gctgcatgac ctcggtccgc cggccgtttc
     1621 tggtgaactg cagccgcgcg aaggccggtg tcgtagtcgc aacgatgagg atcgaggcca
     1681 cgggaaagcc ggtccttggc gcttccgtga cgtagatcga gcccaggatg tgcctctgaa
     1741 agcctccaag ggtttcccgt tcggaaagct ccgccaggtc acggcgtacg gtttccagga
     1801 gatccctgga gactactacc gcgcggtcac cgtgcgtcga gcattcccgc tgcacctcgt
     1861 cgatcatccc aacccctctg catccacggc cgcctccggt ccctcgccgc tccgcctatc
     1921 ggatcccgta gaacccggcc tgcatgtacg agtaccagag gatctcgacg gtccgaaacc
     1981 ccacatcctt gaggacccca agatactcct ccgccgttat cggaaagtat tcgacgtcga
     2041 accgcgccag gtgccgctcc gcctccgcgg gtgatctccc cgaccgctcc tggaaccgcc
     2101 gccatcggct cttgccgatc tccgttcctc gggccgtcag agggcgtatg ttctcgaagg
     2161 tcacgaacag accgccatcg ccaaggagac ggtggcagtt cgcgatggaa gccttgcggg
     2221 actccggatc ctggtagtgc aggctctgga tcgcggtgac gacgtccgcc ggcctcccgt
     2281 tcccgatgct cgtccctccg gcgtccaccg gcgggaggat ggtcaccccg gcgcgtccac
     2341 ccagccgctg gcgggccacc
//
LOCUS       DASRZS010000632         4399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5737157, whole genome shotgun sequence.
ACCESSION   DASRZS010000632 DASRZS010000000
VERSION     DASRZS010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5737157"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VFH83_15385"
     CDS             <1..968
                     /locus_tag="VFH83_15385"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6487809.1"
                     /translation="LRAIPQVRDVVPGLSFTAVASAGGKTTTVLVTALPVARTLGNLS
                     SRGIFQGKDLVPGRSGQILVGRGVARKLSLAPGASVSLFALTSDGGVNTQSYAVSGTT
                     QTIISAVDNVSVFMDLGDAQSLVSSQAVPQLIVFLKKTADTDRVASLLRNAPAGSALS
                     GLTIRTWEQLSPYYRQANGAYQLVLGVARLIVLIVALFSIAGTLSLAVMERLREIGTL
                     RAFGSQRRRVLLMFLFEGLVLGIAGAVIGCLAGGGLSWLIGALGGITMPAQPGTSSGF
                     KVLFTPQTSRFVANGLCVLVVSVVAAVFPGMLASRRRIADLLRAR"
     gene            993..1772
                     /locus_tag="VFH83_15390"
     CDS             993..1772
                     /locus_tag="VFH83_15390"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HET6487810.1"
                     /translation="MRVRTVSFAIAALLAGATFLIGAAGLYAGSAEDLLAQVDARRYL
                     PDFSFVLQMTSLDGDRTVDSNTLWGFVKGSGSENRSLVAFADPASVRGRKMLMDGNIV
                     YLLFPKTTNPIRLSPLQVLMGQASNGDVVRTSFSRDYDVASMTETDRDGVPCYLFALT
                     VKETHKDTSYKRIQLWVEKATLHPLYAEFSTADTLLKKATYRDYRTSLGKDLPFVTDI
                     QDAADPQKHTVMQYVTVGQKVVPETVFRRDYLSSWTPEQPK"
     gene            1769..3055
                     /locus_tag="VFH83_15395"
     CDS             1769..3055
                     /locus_tag="VFH83_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487811.1"
                     /translation="MRPRHLLGVAAVLAVTGARLWALEGQALLDVLPTAALVQAGPSS
                     PWMQSETTAVAAQIAVSHAQTLVEGLSISGTVWGIADSLPSGSPFNTPAAKVDLESRI
                     LELKLTWEMVPGALVWNLGKEVIHPSSGFSRTPLDVLSHGALADTANLTGSAVGAWEE
                     GWIGTGLTAFVGNVTLADYFSPRLSWSSDAAQVLQYVTLPQSDYQNLTQVDFRLGEAD
                     IRVLGLVASGGPGSSDPEVHVQLGSGLDTALGDSVTIRAEICAADAQTRTVVTDSQLQ
                     STTAQSVSWAPRALAGFTWTSAEQLSVMAEYYYNGAGFLGDDYRRLIQYTESLRGSGA
                     PAALGLLDQLGTFSAAQHYGFLRVSGTIDNSLTAAAWTQVNLQDLSGLTGIVLTLGQP
                     KWSVNASLVGAWGGPDTEAGLLPLLWRANLEVSLFF"
     gene            3104..4384
                     /gene="murA"
                     /locus_tag="VFH83_15400"
     CDS             3104..4384
                     /gene="murA"
                     /locus_tag="VFH83_15400"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET6487812.1"
                     /translation="MPEYEVEGDFPLKGRIRASGNKNSALPCLAATLLTDQPVVLRNL
                     PEIEDVHVMIEILKVLGCAVEKLGANEYRFRTLDIRREEVPPHLAHRIRASILFAGPL
                     LARAGRVMLPPPGGDMIGGRPLDTHFLALMELGTRVESGTFFSMTANKLKGAELFMDE
                     ASVTATENAVMAAVLAEGTTVIENAASEPHVQDLCRMLVSMGAGIGGIGSNILRIEGV
                     RKLSGTEFRIGSDYMEVGSLIGLAAATHGELEIDDAEPRNLRMTRLAFGKLGISWETA
                     GGSIRVPATQSLKVVPTMGGQVPKIDDAPWPGFSADIISIALVAATQCEGTVLVHEKM
                     FESRLFYVDKLIAMGARIILCDPHRAVVTGPARLTAAELVSPDVRAGMAMLIAALAAR
                     GKSRIQNVYQIERGYENLTPRLQALGARIRKCGD"
BASE COUNT          685 a         1450 c         1487 g          777 t
ORIGIN      
        1 agctgcgggc catcccccag gtccgggacg tggtccccgg tctctccttc acggccgttg
       61 cgagcgccgg cggaaagacc acgaccgtgc tggtcaccgc gcttcccgtt gccaggaccc
      121 tgggcaacct gagcagcagg ggcatcttcc agggcaagga cctggtgccg ggtcgatcgg
      181 ggcagatcct ggtcggccgc ggagtcgccc gaaagctgag cctggcaccg ggggcttccg
      241 tgtccctttt cgccctgaca tcggatggcg gcgtcaacac ccaatcctac gcggtgtcgg
      301 gaaccaccca gaccatcatc tccgcggtgg acaacgtgtc ggtgttcatg gatctcgggg
      361 atgcccagtc cctggtctcc agccaggcgg ttccccagct catcgtcttc ctcaagaaga
      421 ccgcggacac ggaccgggtg gcttcactcc ttcgtaacgc gccggccggc tcggcgctct
      481 ccggcctcac gatccggacc tgggagcagc tctctcccta ctaccggcag gccaacgggg
      541 cgtaccagct cgtgctggga gttgcgcggc tcatcgtgct gatcgtggcc ctgttctcca
      601 tcgcgggcac gctgtccctc gccgtgatgg agcggcttcg ggagatcggg accctgcggg
      661 cgttcgggag tcagcgccgc cgggtcctgc tcatgttcct cttcgagggt ctggtcctgg
      721 gcatcgcggg agccgtgatc ggctgcctgg ccgggggagg actctcgtgg ctcattggtg
      781 ccctgggcgg gatcacgatg cccgcgcagc ccggcacctc gagcgggttc aaggtgctct
      841 tcacgccgca gacgtcacgg tttgtcgcca acggactctg cgttctggtg gtctcggtcg
      901 tggcggcggt tttccccggg atgctcgcgt cccgcagacg gatcgcggac ctgctccgcg
      961 cccgatagtg gtctggcaga caggagaatg caatgagggt tcgaacggtt tctttcgcca
     1021 tcgccgcgct tctggccggc gcaacgtttc tgatcggtgc cgcgggtctg tacgccggat
     1081 ccgcggagga cctcctcgcc caggtggatg cccgtcggta cctcccggac ttcagctttg
     1141 tgctccagat gacctccctg gacggcgacc gcacggtgga ctccaacacc ctgtggggtt
     1201 tcgtcaaagg aagtggctcg gaaaaccgaa gcctggtggc gttcgcggac ccggcctcgg
     1261 tccggggccg caagatgctg atggacggga acatcgtcta tctgctcttt cccaagacga
     1321 ccaaccccat ccgcctctcc ccgcttcagg tcctcatggg ccaggcatcc aacggggacg
     1381 tggtgcgcac gagcttctcc cgcgactacg acgtggcttc catgaccgag accgatcggg
     1441 atggggtgcc gtgctatctc ttcgcgctca ccgtcaagga gacccacaag gacacctcgt
     1501 acaagaggat ccagctctgg gtggagaagg ccacgctgca tccgctgtac gccgagttct
     1561 ccaccgcgga tacgcttctc aagaaggcga cctaccgcga ctatcgtacc tcgctgggaa
     1621 aggacctgcc gttcgtcacg gacatccaag acgcggcgga cccgcagaag cacacggtca
     1681 tgcagtacgt aacggtgggg cagaaggtgg tgcctgagac cgtctttcgg cgggactacc
     1741 tctcctcctg gacaccggag cagcccaaat gaggcccagg cacttgctcg gcgtggcggc
     1801 cgtcctcgcc gtgacgggcg cccgcctctg ggcgctggaa ggccaggcgc tgctggacgt
     1861 gcttcctacg gccgcgctgg tgcaggccgg cccatcctcc ccgtggatgc agtccgagac
     1921 gacggcggtc gccgcccaga tcgccgtgtc tcacgctcag accctggtcg agggcctgtc
     1981 catctctgga acggtctggg gcatcgccga cagtctcccg tctggatccc cgttcaacac
     2041 gccggcggcg aaggtggacc tggaaagccg gatcctcgag ctgaagctca cgtgggagat
     2101 ggtccccggt gccctggtct ggaaccttgg caaggaagtg atccatccgt cttcggggtt
     2161 ttcgcggacg ccgctggatg tgctctctca cggcgcgctg gcggataccg cgaacctcac
     2221 gggctcggcg gtgggggcgt gggaggaggg gtggatcggg accgggttga cggcctttgt
     2281 ggggaacgtg acgctggccg actacttctc accgcggctt tcgtggtcct ccgacgcggc
     2341 ccaggtcctt cagtacgtga cgctgccgca gagcgactac cagaacctga cccaggtgga
     2401 cttccgtctg ggggaagcgg acatccgagt cctggggctc gttgcgtccg gtgggccggg
     2461 aagcagcgac cccgaggtgc acgtccagct cggctccgga ctggataccg cactgggaga
     2521 ctccgtgacc atccgcgcgg agatctgcgc cgcggacgcg cagacgcgga ccgtagtgac
     2581 cgacagccag cttcagtcca cgacggcgca gtcggtgagc tgggcgccgc gtgcccttgc
     2641 cggtttcacc tggacgagcg cggagcagct ctcggtcatg gcggagtact actacaatgg
     2701 cgcggggttc ctcggggacg attaccgccg gctcatccaa tacacggaga gcctccgcgg
     2761 cagcggggcc ccggcggccc tgggtctgct ggatcagctc gggacgtttt cggccgcgca
     2821 gcactacggg tttctccggg tatccggaac gatcgacaat tcgctcacgg cggcggcctg
     2881 gacccaggtg aaccttcagg atctctccgg attgacggga atcgtcctga cgctcggcca
     2941 gccaaagtgg agcgtgaacg cctccctggt tggcgcgtgg ggtgggccgg ataccgaggc
     3001 cggtctcctg ccccttctct ggagggcgaa cctggaggtg agcctgttct tctagatcga
     3061 aggccgactt gatctcgagc cggtgcccgc tataatccgg gtcatgcccg agtacgaagt
     3121 cgaaggggat tttcctctca agggacggat ccgagccagc ggcaacaaga actccgcgct
     3181 gccctgcctg gccgcgacgc tcctgacgga ccagcctgtg gtgctgcgaa accttccgga
     3241 gatcgaggac gtccacgtga tgatcgagat cctcaaggtc ctcggttgcg ccgtggagaa
     3301 gcttggcgcc aacgagtacc ggtttcggac gctcgacatc cgaagggagg aggtcccgcc
     3361 gcacctggct catcggatcc gcgcctccat tctcttcgcg ggaccgctcc tggcccgggc
     3421 ggggcgggtc atgctcccgc cgcccggcgg ggacatgatc ggcggccgcc ccctggacac
     3481 gcatttcctg gcgttgatgg agctgggcac tcgcgtggag tccgggacct tcttctccat
     3541 gaccgcgaac aagctcaagg gggcggagct cttcatggac gaggcatcgg tcacggccac
     3601 ggagaacgcc gtgatggccg ccgtgctggc cgaggggacc acggtcatcg agaacgcggc
     3661 ctcggagccg cacgtgcagg acctgtgccg gatgctcgtg tccatgggcg ccgggatcgg
     3721 cgggatcggg tccaacatcc tgcggatcga aggggtccgc aagctctccg gaacggagtt
     3781 ccgcatcggc agcgactaca tggaggtggg ctccctgatc ggccttgcgg cggccacgca
     3841 cggggagctg gagatcgatg acgcggaacc ccggaacctc cgcatgacgc gcctggcgtt
     3901 cggcaagctc ggcatctcgt gggagacggc tgggggctcc atccgcgtgc cggccacgca
     3961 gtccctcaag gtggtcccga ccatgggcgg ccaggtcccc aagatcgacg acgctccctg
     4021 gcccggattc tcggcggaca tcatcagcat cgccctggtg gcggccaccc agtgtgaggg
     4081 caccgtgctg gtccacgaga agatgttcga gtcccggttg ttctacgtgg acaagctcat
     4141 cgccatgggc gcgcggatca tcctctgtga tccgcaccgc gccgtggtga ccggaccggc
     4201 ccgtctcacc gcggcggagc tggtgtcgcc ggacgtgcgc gcgggcatgg cgatgctcat
     4261 tgccgcgctc gccgcccggg gcaagagccg gatccagaac gtctaccaga tcgagcgcgg
     4321 ctacgagaat ctcactcccc ggctccaggc gctcggggcg aggatcagga agtgcggcga
     4381 ctgatcaggc gctgatttt
//
LOCUS       DASRZS010000633         2728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_355348, whole genome shotgun sequence.
ACCESSION   DASRZS010000633 DASRZS010000000
VERSION     DASRZS010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355348"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VFH83_15405"
     CDS             <1..538
                     /locus_tag="VFH83_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET6487813.1"
                     /translation="VAAFCIVVTGTLLRGVDIRDAAEAAIQLMSINRYLGTLLAIGLF
                     DAGLLGAICISLASSWAFGEVFGWAHSLNNKIREAPWFYASYIIALATAGTVVLLPGA
                     PLVLITLFVQVIAVTLLPSTLVFLILLLNDKERMGALSNTPTQNVVNVTIVFLIVALS
                     TLYGVSALFPRLFHGGGG"
     gene            543..755
                     /locus_tag="VFH83_15410"
     CDS             543..755
                     /locus_tag="VFH83_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487814.1"
                     /translation="MMRSRDGILTILGGFFSRRFSKFREINRKYATPHVTMTRGVKVA
                     LFLLRIYLLLLVGLLIVKFVTLVVAR"
     gene            767..2038
                     /locus_tag="VFH83_15415"
     CDS             767..2038
                     /locus_tag="VFH83_15415"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6487815.1"
                     /translation="MPETRSTERLPEFFFISQLIKDKVHLSQAGRLQELGRLVDLEIR
                     LAGHYPQVINLIVGRSFGRPPLEIPFSFVQSIDTRRTVVSVPSGTRLQEFEAGPSRIL
                     VKDMVLDKKIIDTDEYEVEVVYDIHLLHAEGKMLVVHVDVTKAGMLRRLHLGWLGHLL
                     WGEVKTADLLPWMYVQPLPTDIDRFRGNVKLTISRERIADIHPVDLADILEELNGEER
                     LAVFDTLDTETAADTLEEAEPRVQRDLVASLRRERLAELFQTMTPAQIADILEILPRI
                     DAQALRKSLPSEAGARVDELMEAHDVKLPSIATSRYLTLPDTALVRQALARFRSRTKR
                     YDVIMYVYVVSEDGTLKGVVDIRELIQNRSEETLHNIMTEQVVTLSPSDTLADSAAEF
                     SKYGFRALPIVDENRKLLGVIRYKDLLAVMQ"
     gene            complement(2278..>2728)
                     /locus_tag="VFH83_15420"
     CDS             complement(2278..>2728)
                     /locus_tag="VFH83_15420"
                     /inference="COORDINATES: protein motif:HMM:NF028226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HET6487816.1"
                     /translation="DVYSSLGVAGGLLVIVALRRLAHLAWASYLDPVVAIAVALVILK
                     ESWAMLSKAFGPLLDSSISPREIATIEACVKRHASTGLHSLRTRSAGGRRHIDFHLSV
                     PETTSVKEAHDLCDQIERDLKRLLPKASVLIHVEPRHATRPAPAPRK"
BASE COUNT          462 a          884 c          889 g          493 t
ORIGIN      
        1 cgtcgccgcg ttttgcatcg tcgtgacggg gacgttgctc cgcggcgtcg acatccggga
       61 cgccgccgag gcggctatcc agctcatgag catcaacagg tacctcggca cgctcttggc
      121 gatcgggctc ttcgacgccg gcctcctcgg cgcgatctgc atatcgctcg cgagctcctg
      181 ggccttcggc gaggtcttcg gctgggccca ctcgctcaac aacaagatcc gcgaggcacc
      241 ctggttctac gccagctaca tcatcgccct ggcgacggcg ggaaccgtag tcctcctccc
      301 cggcgcgccc ctggtgctga tcacgctgtt cgtccaggtc atcgccgtca cgctgctgcc
      361 gtccacgctc gtgttcctca ttctgcttct gaacgacaag gagcgcatgg gcgccttgag
      421 caacacgccg acgcagaacg tcgtgaacgt cacgatcgtc ttcctcattg tggcgctttc
      481 caccctgtac ggcgtgagcg ccctgttccc gaggctcttc cacggaggcg gggggtaggt
      541 cgatgatgcg atccagggac gggattctga ccatcctcgg cggcttcttc agccggcggt
      601 tctcgaagtt tcgcgagatc aaccgcaagt acgctacgcc ccacgtcacg atgacacggg
      661 gagtgaaggt ggcgctcttt ctgctgagga tctatctgct tctgctggtg ggacttctca
      721 tcgtcaagtt cgtcacgctc gtcgtggcgc gataggggat ccaggcatgc ctgagacgcg
      781 gagtaccgaa cggcttcccg agttcttctt catcagccag ctcatcaagg acaaggtgca
      841 tctgtcccag gccgggaggc tccaggaact gggccggctc gtcgacctgg agatccgcct
      901 ggccggccac tatccccagg tgatcaacct gatcgtgggt cgttccttcg ggcggccgcc
      961 gctggagatc ccgttttcct ttgtccagtc gatcgacacc cgtcggaccg tggtctctgt
     1021 gccgtccggg acccggctcc aggagttcga ggccggtccc tcccggatcc tggtcaagga
     1081 catggtcctg gacaagaaga tcatcgatac cgacgagtac gaggtcgagg tggtctacga
     1141 catccacctg ctccacgcgg aaggaaagat gctggtggtc cacgtggacg tgaccaaggc
     1201 cggcatgctc cgccgtctcc acctgggctg gctggggcac ctgctgtggg gcgaggtaaa
     1261 gaccgccgat ctcctgccct ggatgtacgt ccagcccctt ccgaccgata tcgaccggtt
     1321 ccggggaaac gtgaagctca cgatctcccg agagaggatc gcggacatcc acccggtgga
     1381 cctggccgac atcctggagg agctgaacgg ggaggaacgc cttgccgtct tcgacaccct
     1441 ggacacggag accgccgcgg acacgctgga ggaggcggag ccccgggtgc agcgggacct
     1501 ggtggcctcc ctgcgccgcg agcggttggc ggagctcttt cagaccatga ctccggcgca
     1561 gatcgcggac atcctggaga tcctgccgcg catcgacgcg caggctcttc gcaagtccct
     1621 gccctcggag gctggagcca gggtggacga gctcatggaa gctcacgacg tgaagctgcc
     1681 ttcgatcgcc accagccggt acctcacgct ccccgacacg gccctggtga gacaggcgct
     1741 ggcccggttt cgaagccgca cgaagcgcta cgacgtgatc atgtacgtgt acgtggtttc
     1801 cgaggacggg acgctcaagg gggtcgtgga catccgcgag ctgatccaga accgctcgga
     1861 ggagacgcta cacaacatca tgacggagca ggtggtgacc ctgtccccca gcgataccct
     1921 ggcggatagc gcggcggagt tctccaagta cggctttcgc gcgcttccca tcgtggacga
     1981 gaaccgaaag ctcctgggcg tgatccggta caaggacctg ctcgcggtca tgcagtaaga
     2041 ggggtgacgt ctgtcgcgcg acggcgatcg tcagcgcggt cctgccctcg gtgtcctggg
     2101 cgttcgcttt cgcgccgttc gccagggcct gccggatctc ttcggccttc cccgaggccg
     2161 ccagggtgac caggtcggac gcctgggcca cggcaagagc cgggaccagg aggaagggcc
     2221 aagcccagat cccgggcccg ccggggccgc atcgtcattc ttataacctg aaaggtacta
     2281 ctttcggggc gcgggagcgg gccttgtcgc gtgacgcggc tcgacgtgga tcaggacgct
     2341 cgccttgggg aggagccgct tcaggtccct ctcgatctgg tcacacagat cgtgggcctc
     2401 cttgacgctc gtggtctccg gcaccgaaag atggaagtcg atgtgtcgcc ggccgccagc
     2461 gcttcgggtg cggagggaat gcaggccggt gctggcgtgt cgtttgacgc aagcctcgat
     2521 cgtggcgatc tctcggggag agatgctgga atccaggagc gggccgaacg ccttgctcaa
     2581 catggcccag gactccttga gaatcacgag cgcaaccgca atggccacca ccgggtccag
     2641 gtaagacgcc cacgccaggt gggcgagccg ccgcagagcc acgatgacca ggaggccgcc
     2701 ggctaccccg agggaggagt agacgtcc
//
LOCUS       DASRZS010000634         4134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1523640, whole genome shotgun sequence.
ACCESSION   DASRZS010000634 DASRZS010000000
VERSION     DASRZS010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523640"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VFH83_15425"
     CDS             <1..972
                     /locus_tag="VFH83_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET6487817.1"
                     /translation="KELPVPFRRLPYDDAMNLYGSDKPDLRFGLEMKDFSPIAARGGF
                     EVFKKVVEGGGVVRALVAPGCGEYSRKQITELESAAKVYGAAGLAWMKVTEAGLDGGV
                     SKFYTEQGAEIRSALGAKTGDLILMVGAEFRTACVSLGAVRSRLGQELKLADPEKFQF
                     CWIIDFPLFEWNADEKKWDPAHHMFTMPQERFVADFEKRPAEVKGDLYDLVCNGVELA
                     SGSIRIHDPELQKRIFRFLGISDEQAQTRFGFLLEAFRYGPPPHGGIAPGFDRLVAML
                     AGEPTIREVLSFPKNTLGQCPMDDSPSEVDARQLEDLSIRIVPKKAP"
     gene            972..2762
                     /locus_tag="VFH83_15430"
     CDS             972..2762
                     /locus_tag="VFH83_15430"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HET6487818.1"
                     /translation="MDAPRIIASQLGYLCSGRKRVYARAGDIRAGGAQAGSGSPFEIQ
                     DMARVDTESLGALENWKTVYQGAFNRHTGPMGDWLVGDFSSVTRPGLYRAVLPGVTEW
                     SFPFVVSEGAYSGLIPMFLNYVHSQRCGEFESELRGPCHLDDGVRSDTGEAVDAVGGW
                     HDAGDVRKWMVTTPMPILGFFALRDRLGYARNWWGERPHEDDVLAEASWGLRWLLKMQ
                     DPATGMFYEDVGGGGDSRRQPGMSWWHENHAGCYADNSGNCFTDNRLHSGDERQVRVQ
                     YNPIVQYQAVTILMDAVDQFKPHYPAFSLLCRQAALRCWDFLKSRRRDEFHGWTSVLS
                     WRLLAALRLQATGVVADFEISALVSVLLDQQSGDRGFWFMDGARREPYRGILHSAQPL
                     IALGAFIEADYDHPLVGPARDALARCRDRYVLPMTATNPFGIMPYGAFSSRKTEADVY
                     HELPGGLLYRFFMPEHSPQRINHGLSGHWTSWAHGLAVTGRVLESEVCRQAALDQLEW
                     LTGCNPLGVCGVTGVGYRNPTPYSRFYGPLPGGFCVGPRGTEKDEIWMDTEGRTVWSS
                     GEYWMVPIANALLALSNLLPARVLPAGKIG"
     gene            complement(2775..3512)
                     /locus_tag="VFH83_15435"
     CDS             complement(2775..3512)
                     /locus_tag="VFH83_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET6487819.1"
                     /translation="MTEADARRQIVEAGKRLRDRFFVASNDGNISARLSENEILITPT
                     NVNKGDVTEDLILKIDLQGKVISGSLKPSSETKMHLAVYRTRPDVLAIVHAHPPAATG
                     FAACRIRLDQDVILPEVVFGLGKIGFAEYGTPTTDEIPNAVVKEIPDCEALLLANHGA
                     LTVGTDVMKAYYRMEVLEMTARVRLVTRILGEPKPLAEGEVAELHRVRDRQGWGRQAP
                     SLEDVDPRLVDLIAQIVVEIVKKREAP"
     gene            3799..>4134
                     /locus_tag="VFH83_15440"
     CDS             3799..>4134
                     /locus_tag="VFH83_15440"
                     /inference="COORDINATES: protein motif:HMM:NF019826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HET6487820.1"
                     /translation="MPGKKRQDRIERIVEILLSNRGATIKELASSLEVSEMTIRRDLE
                     VLEEDGRIRLVHAGAIPAAELTDLQAGFSLPRESEYQEQVKARVGEKAASLVEAGDVI
                     IIDSGSTAEW"
BASE COUNT          713 a         1296 c         1402 g          723 t
ORIGIN      
        1 aaggagctcc cggtcccgtt ccgccgcctg ccctatgacg acgcgatgaa cctgtacggc
       61 tcggacaagc ccgacctgcg ctttggcctg gagatgaagg acttcagccc gatcgcagca
      121 agaggtggat tcgaggtttt caagaaggtg gtggaaggcg gcggtgtggt gagggcactc
      181 gtggcgccgg gctgcgggga gtactcccgc aagcagatca cggagctgga gtccgcggca
      241 aaggtctacg gcgcggccgg ccttgcatgg atgaaggtca ccgaggcggg gttggacggc
      301 ggagtgtcga agttctacac cgagcagggt gcggagatcc gctccgcgct cggggcaaag
      361 accggcgacc tcatcctcat ggtcggagca gagttccgaa ccgcgtgcgt ctccctgggg
      421 gcggtgagaa gccgcctggg gcaggagctg aagctggctg accccgagaa gttccagttc
      481 tgctggatca tcgacttccc gctgttcgag tggaacgcgg atgagaagaa gtgggacccg
      541 gcgcaccaca tgttcacgat gccccaggag cggttcgtgg cggatttcga gaagcggccc
      601 gcggaggtga agggggacct ctacgacctg gtctgcaacg gcgtggagct ggcatccggc
      661 tccatcagga tccacgaccc cgagctgcag aagcggatct tccggttcct cggcatctcg
      721 gacgaacagg cccagacgcg ctttggcttc ctcctggagg cgttccgcta cgggccaccg
      781 ccgcatggcg gcatagcgcc cgggttcgac cgcctggtgg ccatgctggc gggagagccc
      841 acgatccgtg aggtgctcag cttccccaag aacaccctcg ggcagtgccc catggacgat
      901 tcgccctccg aggtggacgc ccgtcagctc gaggacctgt ccatccggat cgtccccaag
      961 aaggcgccct gatggacgcg ccgcgcatca tcgccagcca gctcggatac ctctgctcgg
     1021 gacgcaagcg ggtgtatgcg cgggccggtg acatccgggc cggcggcgca caggccggct
     1081 caggctcccc cttcgagatc caggacatgg caagagtgga taccgagtcc ctcggagccc
     1141 tcgagaactg gaagaccgtc taccagggag cgttcaaccg gcatacgggc cccatgggcg
     1201 attggctggt gggggacttc tccagcgtga cccggcccgg tctctatcgg gccgtgctcc
     1261 ccggggtgac cgagtggtcc ttcccgttcg tggtttccga gggagcctat tcggggctca
     1321 tcccgatgtt cctgaactac gtccactcgc agcgctgcgg agagtttgaa agcgagctgc
     1381 gcgggccctg tcacctggac gacggcgttc ggtcggacac cggcgaggcg gtggatgccg
     1441 taggaggctg gcacgacgcg ggggacgtga gaaagtggat ggtcaccacc cccatgccca
     1501 tcctgggatt cttcgcgctt cgcgatcggc tcggctatgc gcgcaactgg tggggagagc
     1561 gtccccatga ggacgatgtc ctggcggagg cgtcctgggg gctccgctgg ctgctcaaga
     1621 tgcaggaccc ggccacgggc atgttctacg aggacgtggg cggcggcggt gactcccggc
     1681 gccagcccgg catgagctgg tggcacgaga accacgccgg ctgctacgcg gacaactccg
     1741 gcaactgctt cacggacaac cggctgcaca gcggcgacga gcggcaggtg cgggtccagt
     1801 acaaccccat cgtccagtac caggccgtga ccatcctcat ggacgccgtg gaccagttca
     1861 agccgcacta cccggcattc tccctgttgt gccgccaggc cgcgctccgc tgctgggact
     1921 tcctgaagag ccgtcggcgg gatgagttcc acggatggac ctcggtgctg tcctggaggc
     1981 tcctggccgc gcttcggctc caggcaaccg gcgtggtagc ggatttcgag atctcggccc
     2041 tggtgtcggt gctgctggac cagcagtccg gcgatcgcgg cttctggttc atggacggcg
     2101 cgcgccgcga gccgtaccgg ggaatcctgc actcggcgca gccgctcatc gcgctggggg
     2161 cgttcatcga ggcggactac gatcacccgc tggtggggcc ggctcgggac gctctggccc
     2221 ggtgtcggga tcggtacgtg ctgccgatga cagccaccaa cccgttcggc atcatgccgt
     2281 atggggcctt ctcttcccgc aagacggagg cggacgtcta tcacgagctg ccgggcgggc
     2341 tcctgtaccg gttcttcatg ccggagcatt ccccgcagcg gatcaaccac ggtctctccg
     2401 gtcactggac aagctgggcg catgggcttg cggtcacggg tagagtgctg gagtccgagg
     2461 tttgtcgcca ggcagccctg gaccagctcg agtggctcac gggatgcaac ccgctcgggg
     2521 tctgcggagt caccggcgtt ggataccgca accccacgcc ctatagccgg ttctacggtc
     2581 cgctgccggg agggttctgc gtcggcccgc ggggcaccga gaaggacgag atctggatgg
     2641 atacggaggg ccgcaccgtg tggagctccg gcgagtactg gatggtgccc atcgccaacg
     2701 cccttctggc gctatccaac ctgcttccgg cccgggtgct gccggccggc aagatcggat
     2761 agccgggcca tcgcctaggg cgcctcgcgc ttctttacga tctccacgac gatctgggcg
     2821 atgaggtcca ccaggcgtgg atccacgtcc tccaggctcg gcgcctgtct gccccagccc
     2881 tggcggtcac ggacccgatg cagctctgcc acctcgccct ccgccagcgg cttgggctct
     2941 cccaggatgc gggtgaccag ccgcacgcgg gccgtcatct ccagcacctc catgcggtag
     3001 taggctttca tgacgtccgt gcccacggtc agcgctccgt ggttggccag gagaagcgcc
     3061 tcgcagtccg gaatctcttt cacgaccgcg ttcgggatct cgtctgtggt aggggtgcca
     3121 tactccgcga acccgatctt gcccaggcca aagacgacct cgggcaggat gacgtcctgg
     3181 tccaagcgga tccggcaggc ggcaaagccg gtagcggccg gcggatgggc gtggacgatg
     3241 gccagcacgt ccggccgggt gcggtagacc gcaaggtgca tcttcgtctc tgagctcggt
     3301 ttgaggctcc ccgagatcac cttcccctgc agatcgatct tgaggatgag gtcctcggtc
     3361 acgtctccct tgttcacgtt ggtgggggtg atcaggatct cgttctccga gaggcgagcg
     3421 ctgatgttcc catcgttgga tgcaacgaag aaccggtcac gcaaccgctt ccctgcctcg
     3481 acgatctgtc gcctggcgtc ggcttccgtc atggctttat tgtagtagat cggcgtggtc
     3541 gcgtcgacgg accaaaaaga agacccgcgt ccgatcgcag ccaccccggt gcggggtcgg
     3601 gtggctcccc ccaatgacga tcggaacggc gggcccatga aactgttagc gaagcaacag
     3661 ttccttccga actatagacc attaaataac agttgtcaac gaaagatgag gaaaaaaaga
     3721 agatattctt gacaggagga gattccgaaa cacaatagtg ataggagagt ctcattccag
     3781 gtggagtgaa cacggtccat gccaggtaag aaaaggcaag accgcattga gcgaatcgtc
     3841 gagatccttc tcagcaaccg gggagcgacc atcaaggagc tcgcttcctc gctggaggtc
     3901 tcggaaatga cgatccgtcg agacctggag gtgcttgagg aggacggcag gatccgcctg
     3961 gtgcacgccg gggcgatccc cgcggccgag ctcaccgacc tccaggccgg cttctctctc
     4021 ccgcgggagt cggagtacca ggagcaggtg aaggcccgtg taggggagaa agcggcgtcc
     4081 ctggtggagg ccggggacgt gatcatcatc gattccggct ccacggcgga gtgg
//
LOCUS       DASRZS010000635         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1900040, whole genome shotgun sequence.
ACCESSION   DASRZS010000635 DASRZS010000000
VERSION     DASRZS010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1900040"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VFH83_15445"
     CDS             complement(<1..818)
                     /locus_tag="VFH83_15445"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HET6487821.1"
                     /translation="MTETWNLGVDVGSVSVSAVLVDGAGQPRTWFHRAHRGSPREILA
                     EILKEIGPVVVRGTGLTAGSPSLLESARAVDAQVALIAGARHLVTGARSILFVGGERF
                     GLIRLSADGAYLGTRANSSCAAGTGSFLDQQARRLGFADVSELSRRASACVSPPPRIS
                     TRCAVFARTDLAHAQQQGFSLEEISEGLCKGLAQNIADAILAGERPEGPILFAGGVSL
                     NQSVRKHLEQAMGLPLVTHELGPVLAAFGAAREVMAQADPVGDPAPASLLPETRA"
     gene            complement(931..1509)
                     /locus_tag="VFH83_15450"
     CDS             complement(931..1509)
                     /locus_tag="VFH83_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET6487822.1"
                     /translation="MAQSIKGTRTEQNLMKAFAGESQARNRYTYFSSAARKEGFQQIA
                     GIFQETADNEKEHAKVFFSHLEGGDLEITAAFPAGVVGPTRSNLEAAAAGEKMEWTTL
                     YPQFGKVAREEGFPDVARSFEQVAKAEKFHESRYRALAENIDAGKVFKRVGAVKWHCI
                     NCGYVFEGAEPPKECPACRHPQTFYELLAENY"
     gene            1716..>2159
                     /locus_tag="VFH83_15455"
     CDS             1716..>2159
                     /locus_tag="VFH83_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET6487823.1"
                     /translation="MSSSKFVYLFGGGKAEGNEKMKNLLGGKGANLAEMCNLGVPVPA
                     GFTVTTECCTAYFAGGSKLPSELTPQVTAAIAKTEAIMGMKYGDPNNPLLVSCRSGAR
                     KSMPGMMDTVLNVGLSTATIPGLIKKTNNPRFVWDSYRRLIMMYAD"
BASE COUNT          370 a          716 c          697 g          376 t
ORIGIN      
        1 gcgcgggttt ccggaaggag gctcgcgggg gccggatcgc caacagggtc cgcttgggcc
       61 atcacctccc gagcggctcc aaaggccgcc agcacgggac caagctcgtg cgtcaccagg
      121 ggaaggccca tcgcctgttc caggtgcttg cggacggact ggttgagcga gactccaccg
      181 gcaaagagga tggggccttc gggcctctct ccggccagga tggcgtccgc gatgttctgc
      241 gcgagccctt tgcagagccc ctcgctgatc tcctccaggg aaaagccctg ctgctgcgcg
      301 tgcgccaggt cggtccgagc gaacacggcg cagcgggtgg agatgcgcgg cggcggcgag
      361 acgcaggcgg atgcgcgtcg agacagctcg gatacgtcgg caaagcccag acggcgagcc
      421 tgctgatcca ggaagctccc ggtccccgcc gcgcaggagg agttggcccg ggtgcccagg
      481 tacgcgccat cggcagacag gcggatcagg ccgaaccttt ccccgcctac gaagaggatc
      541 gatcgagcgc ccgtcacgag gtgccgggcg ccggcgatga gggcgacctg cgcgtccacg
      601 gcgcgcgccg actccaggag gctggggctc cccgcggtca gcccggtccc ccgcacgaca
      661 accggaccga tctccttcaa gatctccgcg aggatctccc gagggctgcc gcggtgagcc
      721 cggtggaacc acgtccgtgg ctgtcccgcg ccgtcaacca gcacggcaga cacggagacc
      781 gagccaacgt caactcccag gttccaggtt tcggtcatat gcccaggcga atggccgcag
      841 agcacgactc cggtcgtcgg ctctcgctgc gcggctccgc gcgcggccgt cgccctccgc
      901 acgccttcgg cgtgctacac atccttggcc tcagtagttc tctgcgagca actcgtagaa
      961 ggtctgggga tgcctgcagg ctgggcactc cttcgggggc tcagcgccct cgaacacgta
     1021 gccgcagttg atgcagtgcc atttcaccgc tccgacccgt ttgaacacct ttcccgcgtc
     1081 gatgttctcc gccagggcgc ggtaacgcga ctcgtggaac ttctccgcct tggcgacttg
     1141 ctcgaaggag cgcgccacat cgggaaagcc ctcttcccga gccactttcc cgaactgggg
     1201 atagagggtg gtccactcca tcttctcccc ggccgccgcc gcttccaggt tggaccgggt
     1261 gggcccaacg actccagcgg ggaacgccgc cgtgatctcc aggtcaccgc cctccaggtg
     1321 ggagaagaac accttggcgt gctccttctc gttgtccgcc gtctcctgga agatgccagc
     1381 gatctgctgg aatccctcct ttcgggcggc gctggagaag tacgtgtagc gattgcgcgc
     1441 ctgggactcc cccgcaaagg ccttcatgag gttctgttcc gtcctggtcc ccttgatcga
     1501 ctgtgccatc gtggttcctc ccaaagggca ttctggtgcc tcccaggggt tgtgtcaact
     1561 ttttttcctg accacacgcc cgatggagcc ctggaaagga caactccggt gcggcttgac
     1621 tccctcggag ctcctgggta atcattccgg cgagggtttt tgctggtctt gcggcttgat
     1681 cgctgccaaa tcacgtgaga agggggcggg aagggatgag ctcatcgaaa tttgtatacc
     1741 ttttcggagg cggaaaggct gagggcaacg agaagatgaa gaacctgctg ggcggcaagg
     1801 gagccaacct ggcggaaatg tgcaacctgg gcgtgcccgt gcccgcgggc ttcacggtca
     1861 ccacggagtg ctgcacggcc tactttgcgg gcggaagcaa gctcccgtcg gagctgaccc
     1921 cgcaggtcac tgcagcgatt gccaagaccg aggcgatcat gggcatgaag tatggcgacc
     1981 ccaacaatcc gctcctggtg tcgtgccgct cgggggcgcg caagagcatg cccggcatga
     2041 tggacaccgt gctcaacgta ggcctttcca cagccaccat cccggggctc atcaagaaga
     2101 caaacaaccc gcgcttcgtg tgggattcct accgccgcct gatcatgatg tacgcggac
//
LOCUS       DASRZS010000636         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4974479, whole genome shotgun sequence.
ACCESSION   DASRZS010000636 DASRZS010000000
VERSION     DASRZS010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4974479"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1540
                     /locus_tag="VFH83_15460"
     CDS             191..1540
                     /locus_tag="VFH83_15460"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET6487824.1"
                     /translation="MTMDDRLAKVPGYTTFFGVDELYGHAERVVDRNPGLCRRTIVGA
                     SKDNEPIPMISVGSGKTSILLFASPHPNEPIGAMMAYFLLEELIRDPSLREGRTWHIV
                     PCIDPDGTRSNEGWFAGPFTLANYARHFYRPLGTDQAEWTFPFQYKTFKFDRPIPETR
                     ALMKAIDISRPAVMYALHNSGFGGAYYYISRPLEAAYPAMHRLPKERGLPLSLGEPES
                     PFCVELYPAVYRTTSVSDAYDYYEKFGKGDPAGFMFGGGSSRDYADTVSSPFTLITEV
                     PYFKAAAVGDTTRIGRKRGDVVLEGLDGTAELLTFAQGIVEATLPKLGAGGKFRDASA
                     GFIPLLLKQQDGERAWARSPEIMGQEATSAQLAHALYVGPFYTTLIIAMYRRALKAQL
                     DQTPDPGLEGAYAQLDARIEATMAGIQEGLACTSMPIRDLVQIQYGALLAVLEGLGL"
     gene            1558..>2540
                     /locus_tag="VFH83_15465"
     CDS             1558..>2540
                     /locus_tag="VFH83_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487825.1"
                     /translation="MPENLLEVNDLRTQFATEDGIVTAVDGVSFELPRGGTLGIVGES
                     GCGKSVTSLSIMRLIPNPPGKIVAGRILFEGEDLLLKREREMRAIRGRDISMIFQEPM
                     TSLNPIMTVGDQIIESIRLHRDEGRARARARATEMLGRVGIPTPEKRLGAFPHQLSGG
                     LRQRVMIAMALVSDAKLLIADEPTTALDVTIQAQILRVMKRMRSELHTSIILITHDLG
                     VVAGLADEVVVMYAGRIVERAGVRGIFKEPRHPYTAGLLDSIPSMTRERDRLQAIEGV
                     VPNQLEILPGCPFRPRCARALERCAVEAPQLLPVEAAEASHRAACWNPRPP"
BASE COUNT          430 a          851 c          835 g          424 t
ORIGIN      
        1 cggagtgggg gaacatggtc aacgagggaa aggacttcat cctgctgcac ccggagatga
       61 tcctctggcc cggcctctgc atcctgatcg tggtgctcag cttcaacttc atcggcgacg
      121 cgctgcggga cagcatggat ccccggctgt cgcggcagct ccgataggga gccatggaac
      181 ggagggcggc atgaccatgg atgaccgact ggcgaaggtc ccgggctaca cgacattctt
      241 cggtgtcgac gagctgtatg gccacgccga acgggtcgtt gaccggaatc ccgggctgtg
      301 ccgccggacc attgtcggcg cttccaagga caacgagccc atcccaatga tctccgttgg
      361 cagcgggaag acgtccatcc tcctcttcgc ctctccgcac cccaacgagc ccataggggc
      421 gatgatggcg tacttcctgc tggaggagct gatccgggat ccctccctgc gggagggccg
      481 aacgtggcac atcgtgccgt gcatcgatcc ggacgggacg agaagcaacg agggatggtt
      541 cgccggaccg ttcacgctcg cgaactacgc gcgccacttc taccgtccgt tgggcaccga
      601 ccaggcggag tggacgttcc ccttccagta caagaccttc aagttcgatc gccccatccc
      661 ggagacccgg gccctgatga aggccatcga catcagccgc cccgccgtga tgtatgcgct
      721 tcacaactcg ggctttggag gggcctacta ctacatctcc cgtcccctgg aggccgccta
      781 cccggcgatg catcggctcc ccaaggagcg tggcctgccg ttgtccctcg gtgagccgga
      841 gtcccctttc tgcgtggagc tgtatccggc ggtctaccgg accaccagcg tgagcgacgc
      901 gtacgactac tacgagaagt tcggcaaggg tgaccccgcc ggattcatgt tcggtggggg
      961 gtccagccgc gactacgcgg acaccgtaag ctcgccgttc accctcatca ccgaggtccc
     1021 ctacttcaag gcggccgcgg tcggtgatac cacgcgcatc ggccgcaagc gcggcgacgt
     1081 ggtgctggag ggtctcgatg gcacggcgga gctgctgacc ttcgcccagg ggatcgtgga
     1141 ggcaaccctc ccgaagctcg gcgccggcgg gaagttccgc gatgcttcgg ccggcttcat
     1201 cccgcttctg ctgaagcagc aggacgggga acgagcgtgg gccaggtccc cggagatcat
     1261 gggccaggag gctacctccg cgcagcttgc ccacgccctc tatgtgggtc cgttctacac
     1321 gacgctgatc atcgccatgt accgccgcgc cctcaaggcg cagctcgacc agacgccgga
     1381 ccccggcctg gagggcgctt acgcccagct tgacgcgcgg atcgaggcga cgatggccgg
     1441 gatccaggaa ggactggcgt gcaccagcat gcccatccgg gacctggtcc agatccagta
     1501 cggcgccctt cttgcggtcc tcgaaggcct ggggctctag gacggagagg gcgggccatg
     1561 ccggagaacc tcctcgaagt caacgacctt cgtacccagt tcgcgaccga ggacggaatc
     1621 gtcacggcgg tggatggcgt cagcttcgag ctgccgcgcg ggggcaccct cgggatcgtc
     1681 ggggagtccg gctgcggcaa gagcgtcacg tccctctcca tcatgcggct gatccccaac
     1741 ccgccaggga agatcgtggc gggacgcatc ctgttcgaag gggaggatct gctcctgaag
     1801 cgggagcggg agatgcgcgc cattcgaggc cgggacatct ccatgatctt ccaggagccg
     1861 atgaccagcc tcaacccgat catgaccgtc ggggaccaga tcatcgagtc catccgcctc
     1921 caccgggacg aggggagggc ccgggcccgc gcccgcgcca cggagatgct tggtcgggtc
     1981 ggcatcccga ctccggagaa gcgcctcggc gcctttcccc accagctttc cggcggtctc
     2041 cgccagcggg tgatgatcgc catggcactg gtgagcgatg ccaagcttct catcgcggac
     2101 gagccgacga ccgccctcga cgtcaccatc caggcccaga tccttcgagt catgaagcgg
     2161 atgcgatccg agcttcacac ctcgatcatc ctgatcacgc acgacctggg cgtcgttgcg
     2221 ggcctcgcgg atgaggtggt ggtgatgtac gcggggagga tcgtcgagcg ggcgggcgtc
     2281 cgtggcatct tcaaggagcc gcggcacccg tacacggcgg ggcttctcga ctccattccg
     2341 agcatgacca gggaacggga tcgtctccag gccatcgagg gagtcgtgcc gaatcagctc
     2401 gaaatccttc cgggctgccc gtttcggccc cgttgcgcgc gcgctctgga gcgctgtgcc
     2461 gtggaggcgc cgcagctcct tcccgttgag gcagccgagg cgtctcaccg tgcggcgtgc
     2521 tggaacccac ggccgccgag
//
LOCUS       DASRZS010000637         5142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3061121, whole genome shotgun sequence.
ACCESSION   DASRZS010000637 DASRZS010000000
VERSION     DASRZS010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3061121"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="VFH83_15470"
     CDS             complement(<1..1121)
                     /locus_tag="VFH83_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET6487826.1"
                     /translation="MKETKNKEIREQAERTRAKRMAWWHEARFGMFVHWGLYSQIGRH
                     EWVMNRERIPVEEYEKLADTWKPKERPMREWAKLAVDAGMKYMVMTTKHHEGFCLWDT
                     KQTDYNAVRRGPGRDLVQEYVEACREAGLRVGFYYSLMDWHHPDGARCATHEKSRRRF
                     LDFTQGCVEELMSNYGTIDILWYDVSWPLSSPKLWESAKMNAMVRRLQPKIIINNRSQ
                     LDEDFGTPEEQIQAAAAGRAWEACMTFNGSWGYFPTAPDSDWHSTRAVIGMLQNVAAG
                     GGNLLLNIGPAPDGSVPRQAYDRLLPVGQWLGKHGEAVYGAVDRIDGVLERWLNHGSW
                     TLKGTTAYYWTGRWVGSEIVIGGLKTKVKRVTVLETGANV"
     gene            complement(1225..2175)
                     /locus_tag="VFH83_15475"
     CDS             complement(1225..2175)
                     /locus_tag="VFH83_15475"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HET6487827.1"
                     /translation="MERIILDTDIGDDIDDAFALTLVVRSPELSLAGVTTVFRNAERR
                     AKLSLKLLSTLGVSGIPVLAGCDTPFVEGFRAQDRDTWDPEGRFIPCQYATDYDGIRL
                     PSDEHAVDFIIRTVREQPGQIVLVTIGPLTNAAMALRKAPDIASKLKSITMMGGYRSE
                     NAPEWNILCDPEAARIVFTAGAPIAMVGLDVTLQCSLSLQEVDRLRQAGSDGLGLLYE
                     LTQKWLAHSGAKNPILHDPLAVATLIDESVVRFEPRLVQVALEGTARGCTLTRTPPDP
                     AKDRHYGKGRPEQPPCRVASAVDAARFKTLFLGRVLSGSR"
     gene            complement(2185..4203)
                     /locus_tag="VFH83_15480"
     CDS             complement(2185..4203)
                     /locus_tag="VFH83_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HET6487828.1"
                     /translation="MAITARDGKLLSRFDGELLQIEPWGENALRVRATCASGLQADRL
                     WALLPKRAPNPSAVAVTASMSGAEGSITNGRITARLERSGRIAFLNAKGEVILEERWR
                     TRGMRGESFYSTHVVARELRPLTGGAYRITARFEAQDDERIFGMGQYQDGVLNLKGSM
                     LELAQRNSQCSVPFCVSSRGYGFLWNNPAVGRVTFARNMTEWVAETTPELDYWITAGD
                     TPAQIEESYAAVTGTVPMMPDFAMGFWQCKLRYRTQEELLGIAREYKKRGLPLSVIVV
                     DFFHWPLQGDWTFDSRYWPDPAGMVRELEGMGIKLMVSIWPTVDRRSANYPVMLEKGY
                     LTRADRGIRTMMDFNGNTVFADLTNPEAREFVWGQVKKSYWDKGIRIFWLDVAEPEFA
                     IYDFDNYRYHLGSEMEVGNHYPMAYAQTFYDGMRRAGQDQVINLIRCAWAGSQRYGAL
                     VWSGDITTTWPTFRRQLVAGLNMAMAGIPWWTTDIGGFEGGDPDDPAYRELLIRWFQW
                     GAFCPVFRLHGFREGPAPAVEMSGGSSSDDVFSGGPNEVWSYGDEAYGILTKYLRIRE
                     TMRPYIGRLMREASQKGTPVMRPLFYDFPGDPKAWDVEDQFLFGPDVLVAPVLYPGMR
                     KRSVYLPAGESWKESRTGVSHPGGRTVEVDCPLDTIPVLTRGNTSPLG"
     gene            4298..>5142
                     /locus_tag="VFH83_15485"
     CDS             4298..>5142
                     /locus_tag="VFH83_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487829.1"
                     /translation="MQHPTTTHRERLEAVFAGRAPDRTPFLGGWIACPEHICGLAGAT
                     LADYWSDPFSWSVKAYQALGCDGLIDLFVPKSQSDFRCVDASSYLHAQTDLSLEEVVA
                     AVEAMPYPSEIERSFDFEREYAAYRKALVDRQAQSEDMVWMPANWNAGGRLSWYGDFG
                     YQSYFELIGLYPKLAEKLIAVGGARGYCQGRLIARAVEEGLYPHAVLLGEDICTQRGP
                     MISLQLLRNHYAPALARGLEPLQRVGCRPVWHSDGDVRPMVDLLIDCGIEGFQGFQPE
                     CGMTL"
BASE COUNT          826 a         1747 c         1663 g          906 t
ORIGIN      
        1 acattcgcgc ccgtctcgag caccgtgacc cgcttcacct tggtcttcag ccctccgatg
       61 acgatctccg accccaccca tcggccggtc cagtagtagg cggtcgtccc cttcaacgtc
      121 caggacccgt ggttgagcca acgctccagg actccgtcga tccggtccac ggcgccatag
      181 acagcctccc cgtgtttgcc cagccattga cccaccggca gcaggcggtc gtaggcctgt
      241 cggggaaccg atccgtcggg agccggtcca atgttgagga ggaggttccc gccgccggcc
      301 gccacgttct gcagcatgcc gatcacggcg cgggtggagt gccagtcgct gtccggcgcg
      361 gtcgggaagt agccccagga gccgttgaag gtcatgcacg cttcccaggc ccgacccgcc
      421 gccgcggcct ggatctgctc ctccggcgtt ccgaaatcct cgtcgagctg ggaccggttg
      481 ttgatgatga tcttgggctg cagacgccgg accatggcgt tcatcttggc gctctcccag
      541 agcttggggg aggagagcgg ccaggagacg tcgtaccaca ggatgtcgat ggtgccgtag
      601 ttgctcatca gctcctcgac gcatccctgg gtgaagtcga ggaagcgacg gcgggacttc
      661 tcgtgcgtgg cgcaccgggc cccatccggg tgatgccagt ccatcaggga gtagtaaaac
      721 cccacccgaa gaccggcttc gcggcaggcc tccacgtact cctggaccag gtcgcggccc
      781 gggccccggc gcaccgcgtt gtagtccgtc tgcttcgtgt cccagagaca gaagccttca
      841 tgatgcttgg tggtcatcac catgtacttc atgccggcgt ccaccgcgag cttggcccac
      901 tcccgcatgg gccgctcctt gggcttccac gtatccgcca gcttctcgta ttcctccacc
      961 gggatccgct cccggttcat gacccactca tgccgcccga tctgggagta cagaccccag
     1021 tgcacgaaca tcccgaagcg agcctcgtgc caccacgcca tgcgcttggc gcgtgtgcgc
     1081 tcggcctgct cgcggatctc cttgttcttc gtttctttca tcgaacgctc cttggccggt
     1141 agggtatacg gattcgaacg tcctgcctac cgtattcggt cttgttccgc tccgcgctgg
     1201 atgcctttgc cggcggcgcc ctccttaccg tgacccgctg agcacccggc ccaggaagag
     1261 cgtcttgaac cgggccgcgt ccaccgcgct ggccacgcgg catgggggct gctccggcct
     1321 gcctttcccg tagtgccggt ccttggccgg gtccggcggg gtccgggtca gcgtgcaacc
     1381 ccgggccgtc ccctccagcg ccacctgcac cagccgaggc tcgaaccgga ccaccgactc
     1441 gtcgatcaga gtggctaccg ccagcggatc gtgcaggatg ggattcttcg cgccggagtg
     1501 ggccagccac ttctgggtca gctcgtagag gaggcccaga ccgtcggagc ctgcctggcg
     1561 cagccgatcc acctcttgga gcgagagcga gcactgcagc gtcacgtcca ggccaaccat
     1621 cgcgatgggc gcgcctgccg tgaacacgat ccgggcggct tcgggatcac agaggatgtt
     1681 ccactccgga gcgttctctg accgatagcc gcccatcatc gtgatggact tgagcttgga
     1741 ggcgatgtcc ggggccttgc ggagcgccat ggcggcattc gtcaggggac cgatggtcac
     1801 caggacgatc tggccgggct gctcacggac cgttcggatg atgaagtcca ccgcgtgctc
     1861 atccgagggc agacgaatcc cgtcgtagtc cgtcgcgtac tgacacggga tgaacctccc
     1921 ctctggatcc caggtgtccc gatcctgggc gcggaaccct tccacgaagg gcgtgtcaca
     1981 gccggccagc accggaatgc cggagacccc cagggtggaa aggagcttca gggagagctt
     2041 cgcgcggcgc tcggcgtttc ggaacaccgt ggtgacgccg gccagcgaga gctctggcga
     2101 ccggacgacc agggtaagcg cgaaggcatc gtcgatgtca tcgccaatgt ccgtgtccag
     2161 gatgatccgt tccatggggg cctcctatcc caggggcgat gtgttgcccc gcgtgagcac
     2221 ggggatggtg tccaaagggc agtcgacttc cacggtccgt cctcccggat gggagacgcc
     2281 cgtgcggctc tccttccagg actcgccggc ggggaggtac acggagcgct tacgcatccc
     2341 cgggtagagc accggcgcca ccaggacgtc cggaccgaag agaaactggt cctccacgtc
     2401 ccacgccttg ggatcccccg ggaaatcgta gaacagggga cgcattacgg gcgtgccttt
     2461 ctggctcgcc tcccgcatga gtcgcccgat gtacggcctc atcgtctcgc ggatgcgcag
     2521 gtacttcgtg aggatcccgt acgcctcatc cccatagctc cagacctcgt tcggcccacc
     2581 cgagaacacg tcatcgctcg agcttcctcc gctcatctcc acggcgggcg ccggtccctc
     2641 gcggaaaccg tggagccgga agacgggaca aaacgcgccc cactggaacc agcggatgag
     2701 gagctcccga taggcggggt cgtcgggatc tcctccctcg aagccgccga tgtccgtggt
     2761 ccaccagggg atcccggcca tcgccatgtt caggccggca acgagctgtc ggcggaaggt
     2821 aggccacgtg gtggtgatgt cgccggacca caccagcgcg ccgtagcgct ggcttccggc
     2881 ccacgcgcag cggatgagat tgatgacctg gtcctggccc gcccgccgca tcccgtcgta
     2941 gaaggtctgg gcataggcca tcggatagtg gttgcccacc tccatctccg atcccaggtg
     3001 gtagcggtag ttgtcgaaat cgtagatggc gaactccggc tcggcaacgt ccagccagaa
     3061 gatccggatg cccttgtccc agtaactctt cttcacctgg ccccagacga actcccgcgc
     3121 ctcgggattg gtcaggtcgg cgaacaccgt gttcccattg aaatccatca tggtgcggat
     3181 cccgcgatcg gcgcgcgtca ggtacccctt ctccagcatc acgggatagt tggcgctgcg
     3241 gcggtccacc gtgggccaga tggagaccat gagcttgatg cccatgccct cgagctctcg
     3301 gaccatgccc gccgggtcgg gccagtatcg ggagtcgaac gtccagtcgc cttgcagggg
     3361 ccagtggaag aagtccacca cgatcaccga gagcggaagg ccgcgtttct tgtactcccg
     3421 cgcgatcccc agcagctcct cctgggtccg gtagcggagc ttgcactgcc agaagcccat
     3481 ggcgaagtcc ggcatcatag ggacggtgcc ggtgacggcg gcgtagctct cctcgatctg
     3541 cgccggggtg tcaccggcgg tgatccagta gtccagctcc ggggtcgtct ccgccaccca
     3601 ctccgtcatg ttgcgggcaa aggtgacccg tcccacggcc ggattgttcc agagaaagcc
     3661 gtagccccgg ctcgatacgc agaaggggac gctgcactgg gagttgcgct gggcaagctc
     3721 cagcatggat cccttcaggt tcaggacgcc gtcctggtac tgacccatgc cgaagatcct
     3781 ctcgtcgtcc tgcgcctcga atcgagccgt gatccggtag gcgccgccgg taagcggcct
     3841 gagctcgcgc gccaccacgt gggtggaata gaaggactct ccgcgcatcc cgcgggtcct
     3901 ccaacgctcc tcgaggatga cctcgccctt ggcgttgagg aaggcgatcc ggccgctgcg
     3961 ctccagccgc gccgtgatcc gtccattcgt gatggagcct tccgcgccgc tcatggaagc
     4021 ggtgacggca accgccgacg ggttcggcgc tcgcttcggc agcagcgccc agagccggtc
     4081 cgcctggagc ccggaggcgc aggtggcgcg gacccgaagc gcgttctcgc cccagggttc
     4141 gatctggagc agctcgccgt cgaagcggga aaggagcttg ccgtcccgag cagtaatggc
     4201 catgagtgtc ctccggtgcc acacggtagc gtcgctccgg gcgcacggtc aagccgcgct
     4261 tgaaggccgg gaccaaatcc gctatacggt atccatcgtg caacacccta cgacgacgca
     4321 ccgcgagcgg ttggaagcgg tctttgccgg ccgcgcgccg gaccgcactc ctttcctggg
     4381 cggctggatc gcgtgccccg agcacatctg cggcctggcc ggcgccaccc tggcggatta
     4441 ctggtccgac ccgttctcct ggtccgtgaa ggcgtaccag gcgctgggct gtgacgggct
     4501 catcgacctc ttcgtcccga agtcgcaatc ggacttccgc tgcgtggatg caagctccta
     4561 cctgcacgcc cagacggacc tgtccctgga ggaggtggtg gctgccgtgg aggccatgcc
     4621 ctacccgtcc gagatcgagc ggagcttcga cttcgagcgt gagtatgcgg cctatcgcaa
     4681 ggccctggtg gatcggcagg cccagtccga agacatggtc tggatgccgg ccaactggaa
     4741 tgccgggggc cggctgagct ggtacggcga tttcggctac cagagctact tcgagctgat
     4801 cggcctgtac ccgaagctgg cggagaagct catcgccgtc ggcggggcgc ggggctactg
     4861 ccagggacgg ttgatcgccc gggccgtgga ggaagggctg tacccccacg cggtgctcct
     4921 gggagaggac atctgcaccc agcgcggacc catgatctcc ctgcagctcc ttcgcaacca
     4981 ctacgcgccg gccctggcgc gcggcctgga gccgctccag cgggtcggct gccggccggt
     5041 ctggcattcc gacggcgacg tgagaccgat ggtggatctc ctgatcgact gcggcatcga
     5101 gggcttccag gggtttcagc cggagtgcgg catgacgttg ga
//
LOCUS       DASRZS010000638         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3423016, whole genome shotgun sequence.
ACCESSION   DASRZS010000638 DASRZS010000000
VERSION     DASRZS010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3423016"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1546)
                     /locus_tag="VFH83_15490"
     CDS             complement(<1..1546)
                     /locus_tag="VFH83_15490"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487830.1"
                     /translation="MALLEVRDLQLHYDTAQGTVRAVDHVSFSVPSTGQAVGLIGETG
                     SGKTSVLLALTRMLPKNVSLLSGEVLLEESNVLDMSEERFRRDIRWKRIAVVPQGVQN
                     GFNPVIRVGEQVMERALAEASADADAVREEARRLLRTVGLPEGIWNRYPHELSGGMKQ
                     RAAIAMALTMRPTLLLLDEPTSALDVSIQAQIMNVLKKLKWDLGISMVFISHDIALAS
                     DLCDRFVVLSHGSVREEGTAEQVLLEPRDRYTQELLASVPRLKRADRRRPARPTDSQG
                     PALVSAERLAVRFTVRAGFFRSTIVRAVDGVSLSLGRAETVALVGESGSGKTTLGRAL
                     LRLLAPASGTVRFDGQEITRLRGAELKRLRRRAQAVFQDPYASISPFMNVRQIVEEPL
                     EVHGVRGARERAEVVSHALEAVKLSPAGAFLEKHPHTLSGGQRQRVCIARALALTPQL
                     IVADEPVSMVDASNRAEILRLLEDLQRERGISFLYITHDIASARQFADRIAVMYLGTI
                     VEEGAAA"
     gene            complement(1534..>2189)
                     /locus_tag="VFH83_15495"
     CDS             complement(1534..>2189)
                     /locus_tag="VFH83_15495"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487831.1"
                     /translation="AAGQPQSHPDQGACYRRGDADEEGDGQAEEEALGEVPAQRIRAQ
                     RSADIVANVPLLPLLIFLVFIGGSRLWLILLVLVLFSWPGLTITVRTMVLQLRSGPEV
                     EAAQALGASGRRVVVRHILPHTASFVLAQLVFSAPSAILAEAGLSFLGLGDPSLPTWG
                     QILESGFRTGAVYLGYWWWVIPPGLAIVITAVTFMFLALGLEPVVDPRLRGRRLWRS"
BASE COUNT          372 a          752 c          735 g          330 t
ORIGIN      
        1 ccgccgcggc cccctcttcc acgatggttc ccaggtacat gacggcgatc ctgtccgcga
       61 actgtcgggc gctggcgatg tcatgggtga tgtacaggaa ggagatgccg cgctcccgct
      121 gcaggtcctc caggagcctg aggatctccg cccggttgga tgcgtccacc atggagaccg
      181 gttcgtccgc cacgatcagt tgcggcgtca gggccagggc tcgggcgatg caaacgcgtt
      241 ggcgctgtcc tccggagagg gtgtgcgggt gcttctccag aaatgcgccc gcgggtgaaa
      301 gcttcactgc ctcaagggca tgggagacca cctccgcgcg ctctcgggct cctcggacgc
      361 catgcacctc gagcggctcc tccacgatct ggcggacgtt catgaacggg ctgatgctgg
      421 cgtagggatc ctggaacacc gcctgcgcgc gcctgcggag cctcttcagc tccgctcccc
      481 gaagtctcgt gatctcctga ccatcgaacc ggaccgtccc ggaggcaggc gccaggagac
      541 gcagcagcgc tcggcccagc gtggtcttgc cgcttcccga ctctcccacg agcgccacgg
      601 tctccgctct gcccagggac aaggacacgc cgtccaccgc ccgcacgatc gtggatcgga
      661 agaatccggc ccggaccgtg aaccgcacgg ccagtcgctc agcgctcacc agcgccggac
      721 cttgggaatc cgtgggacga gcaggccggc gacggtccgc tcgcttcagc ctcggcacgc
      781 ttgccaggag ctcctgggta tagcggtccc gcggctccag gagcacctgc tccgccgtcc
      841 cctcctcccg caccgacccg tgagacagca cgacaaaccg gtcgcacagg tcgctggcca
      901 gggcgatgtc gtgggagatg aacaccatgg agatcccgag gtcccacttg agcttcttga
      961 gcacgttcat gatctgggcc tggatgctca cgtccagcgc cgacgtgggc tcgtccagga
     1021 gcaggagggt gggccgcatg gtgagcgcca tggcgatggc cgctcgctgc ttcatgccgc
     1081 cggaaagctc gtgcgggtag cgattccaga tgccttccgg caaaccgacc gtgcgcagga
     1141 ggcggcgggc ctcttcccgg acggcgtcgg catccgccga tgcttcggcc agggcgcgct
     1201 ccatgacctg ctccccgacg cggatgaccg ggttgaagcc gttctgcacc ccctggggca
     1261 ccacggcgat cctcttccag cggatgtccc tccgaaaccg ctcctcgctc atgtccagga
     1321 cgttgctctc ttccagaagc acctcgccgg agagcaggct gacgttcttg gggagcatcc
     1381 gcgtcagggc caggagcacg ctggtcttgc cgctgccggt ctcgccaatc agcccgaccg
     1441 cctggccggt cgacggtacg gaaaaggaga cgtgatccac cgcgcgcacg gtcccctggg
     1501 ccgtatcgta gtggagctgg aggtcccgga cctctaggag cgccaaaggc gtcgtcctcg
     1561 gagccgggga tccaccaccg gctccagacc cagggccagg aacatgaagg tgaccgcggt
     1621 gatcacgatg gcgaggcccg gtgggatcac ccaccaccag tacccgagat agaccgcgcc
     1681 cgtgcggaag ccgctttcca ggatctggcc ccaggtgggg agggacgggt cgcccaggcc
     1741 caggaagctc aggcccgctt cggccaggat ggccgaaggc gcggagaaca cgagctgggc
     1801 cagcacgaag gaagcggtat gcgggagaat gtggcggacc accacgcgtc ggccggatgc
     1861 tcccagcgcc tgggcagcct ccacctccgg accggaccga agctgcagga ccatggtccg
     1921 caccgtgatg gtcagtcccg gccagctgaa gaggaccagc accaggagga tgagccacaa
     1981 tcgggacccg ccgatgaaga ccaggaagat gagaaggggg aggagcggga cattggcaac
     2041 gatgtccgcg gagcgctggg cacggatgcg ctgggccggg acctcgccca gggcctcctc
     2101 ttcggcctgc ccatcgccct cctcatcggc atctccacgg cggtagcagg caccctgatc
     2161 gggatgggac tggggctggc cagcggcta
//
LOCUS       DASRZS010000639         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1904473, whole genome shotgun sequence.
ACCESSION   DASRZS010000639 DASRZS010000000
VERSION     DASRZS010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904473"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VFH83_15500"
     CDS             complement(<1..520)
                     /locus_tag="VFH83_15500"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487832.1"
                     /translation="MEARDSGGGFRSRLPIALTHAWPMIIIYAVFVVMVLILGAISPF
                     FSSPVSVTNLFAAALPLAFAAMAQTMVVLVKGIDLSVGPTMSLVIVVMASLMHDSAVS
                     IVGILLLSVALGLAIGVINGAFVVFARLQSIIVTLAMSSILSGIALYILPEPGGYVPE
                     GLGAVGGMSFGAI"
     gene            complement(507..2027)
                     /locus_tag="VFH83_15505"
     CDS             complement(507..2027)
                     /locus_tag="VFH83_15505"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6487833.1"
                     /translation="MDPVLLNLAGVSKSYAGNFAIQDIALEIRAGDVHALVGENGAGK
                     STLVKIISGGIAADSGVVTYRERDYRPRSPFEAQQAGIVAVQQELSLSPYLPVYQNLW
                     LGHESSRGSLFIRMRDLRRRAVDLCSRYGVHANIEAWVSELSLEEQQVVEVLKAVALE
                     PRLIILDEPTSALGSANTRWLLDLVRRLKDQGCAVLFISHRLSEVMEASDEITVLKDG
                     RAVATVRRADVTEADVVRMMVGRDLKDIFPPKLDPGPRQSLPVILEVDRLRTDRVQDV
                     SFTIRRGEVVGLAGLEGQGQHELILALFGLKRIAGGNLTLQGQAIREHSPSASVRRRV
                     GLVPVDRRTEGVILPLSVSENIAHATLGLRSRAGWVDRRREAALVQGFMKDLAIRAPG
                     ARSPVGDLSGGNQQKVALGKWLALDPRFLLLDDPTRGVDIETRRDIYHRIRALASQGV
                     GILLNSTDAIELVGICDRVLVMYEGRIVRELSGPEITEGGIVGAAVGVQGGTNGSS"
     gene            complement(2029..>2827)
                     /locus_tag="VFH83_15510"
     CDS             complement(2029..>2827)
                     /locus_tag="VFH83_15510"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6487834.1"
                     /translation="ANVEKQIAQINDMIDKHVDGIVVMAASDTAENPVLEAAMKAGIP
                     VVTSDHYVTSTKLTCQIYVDQLQWGRVTAQWLVDQLGPGGGNIVVLNGVAGNADNNGR
                     WGGAKEVFDKHPEIKILAMANADWDQAKAQQAVANLLPSFPKIDGIWSQGGAMTLGAV
                     YAFQAANRPLVPMAGEANNGLLKVWKKELANGFDSVAPACPSTMGAMALDNAVKVLGG
                     QKMAKATLLTPPVITAETLDQYVRPDLSDALWLPTILSPEQLQALFH"
BASE COUNT          478 a          955 c          944 g          450 t
ORIGIN      
        1 gaatggcacc gaacgacatg cccccgacag cgcccaggcc ttcgggcacg tagccgccgg
       61 gctccggcag gatgtacagc gcgatccccg acaggatgga cgacatggca agcgtcacga
      121 tgatggactg aaggcgcgca aacacaacga acgcgccgtt gatgacgccg atggccaggc
      181 cgagggccac ggacaggagc aggatgccga caatgctcac ggccgagtcg tgcatcagcg
      241 aggccatcac cacgatgacg aggctcatcg tggggcccac ggagagatcg atccccttca
      301 cgaggacgac catggtctgg gccatggcgg cgaaggcgag cggcagcgcg gcggcaaaga
      361 gattcgtcac ggacacgggg gaggagaaga acgggctgat ggctcccagg atgaggacca
      421 tgaccacgaa gacggcatag atgatgatca tcggccaggc atgcgtgagc gcgatgggca
      481 ggcgcgaacg gaagccgcct ccggagtcac gagcttccat tcgtcccccc ttgtactccc
      541 acggcggcgc cgacgatgcc gccctccgtg atctccggac ccgacagctc ccggacgatc
      601 cttccctcgt acatgaccag cacgcggtcg cagatgccca ccagctcgat ggcgtccgtg
      661 gagttcagga ggattccgac cccctgggac gccagtgccc ggatgcgatg gtagatgtcc
      721 cgacgcgtct cgatgtcgac gccacgggtg ggatcgtcca gcaggaggaa ccgggggtcc
      781 agcgccagcc acttcccgag ggcgaccttc tgctggttgc cgccgctcag gtcccccacg
      841 gggctgcggg ccccgggcgc gcggatggca aggtccttca tgaagccctg cacgagcgcg
      901 gcttcccggc gacggtccac ccagccggcc cggcttcgaa gtcccagcgt ggcgtgggcg
      961 atgttctccg acacgctgag cggcaggata acgccttccg tgcgccggtc cacgggaaca
     1021 agacccaccc ggcgacgcac ggaagcggac ggagaatgct cccggatggc ctgcccctgc
     1081 agcgtgaggt ttccgccggc aatccgcttc aggccgaaca gggccaggat cagctcgtgc
     1141 tggccctgcc cctccagccc ggcgagcccg accacttccc cccggcggat cgtgaacgac
     1201 acgtcctgca cccggtcggt cctcaggcgg tccacctcca ggatgaccgg cagggactgg
     1261 cggggaccgg ggtccagctt cggtgggaag atgtccttca ggtcccggcc caccatcatg
     1321 cgaacgacgt ccgcctccgt gacgtcggcg cgccggacgg tggcgaccgc gcggccgtcc
     1381 ttcagcaccg tgatctcgtc cgaggcttcc atcacttcgc tcaggcggtg ggagatgaac
     1441 agcacggcgc acccctggtc cttcaggcgg cggacgaggt ccaagagcca gcgcgtgttg
     1501 gcggaaccga gcgcggaggt cggctcatcc aggatgatga ggcgcggctc cagcgcgacc
     1561 gccttcagga cctccaccac ctgctgttct tccagggaga gctcggatac ccaggcttcg
     1621 atatttgcat gaacgccgta gcgcgagcac agatccacgg cgcgccgccg cagatcccgc
     1681 atccggatga acaggctgcc tcgcgagctc tcgtgcccga gccagaggtt ctggtagacg
     1741 ggcaggtacg ggctgaggga cagctcctgc tggaccgcca cgatgccggc ctgctgcgcc
     1801 tcgaacggag accggggacg gtagtcgcgc tcccggtagg tcaccacgcc gctgtccgcc
     1861 gcgatgccgc cggagatgat cttcacgagc gttgacttgc cggcgccgtt ctctcccacc
     1921 agcgcgtgca cgtctcctgc gcggatttcc agcgcgatgt cctggatggc gaagttgccg
     1981 gcgtaagact tgcttacgcc ggcaagattc aggaggaccg ggtccatcct agtggaatag
     2041 cgcctgaagc tgctccgggc tgaggatcgt cgggagccac agggcatccg agagatccgg
     2101 ccgcacgtac tggtccagcg tttccgccgt gatgaccggc ggggtcaaca gcgtagcctt
     2161 cgccatcttc tggcctccga ggaccttcac cgcgttgtcc agcgccatgg cccccatggt
     2221 ggaggggcag gcgggggcga cggaatcgaa gccgttggcc agctccttct tccacacctt
     2281 cagcaggccg ttgttggcct cacccgccat cgggacgagc gggcggttgg cggcctggaa
     2341 cgcgtagacg gcgccgagcg tcatggcccc gccctgggac cagatgccgt cgatcttggg
     2401 gaacgagggc aggaggttgg ccaccgcctg ctgcgccttg gcctggtccc agtccgcgtt
     2461 ggccatggcg aggatcttga tctcggggtg cttgtcaaag acttccttgg cgccgcccca
     2521 gcggccgttg ttgtccgcgt ttcccgccac gccgttcagc acgacgatgt tcccgccgcc
     2581 cgggccgagc tggtccacga gccactgcgc ggtgacacgg ccccactgca gctggtccac
     2641 gtagatctgg caggtcagct tggtggaagt cacgtagtgg tccgaggtga ccacggggat
     2701 ccccgccttc atggcggctt ccagcaccgg gttctccgcg gtgtcggagg cagccatcac
     2761 cacgatgccg tccacgtgct tgtcgatcat gtcgttgatc tgcgcgatct gcttttccac
     2821 gttggca
//
LOCUS       DASRZS010000640         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3827451, whole genome shotgun sequence.
ACCESSION   DASRZS010000640 DASRZS010000000
VERSION     DASRZS010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3827451"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1032)
                     /locus_tag="VFH83_15515"
     CDS             complement(118..1032)
                     /locus_tag="VFH83_15515"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET6487835.1"
                     /translation="MDRPELAGMRLDLFLSEGLGLFSRSQARGRVQEALVNGKPARLS
                     KRLKLGDVVRVRWSEAPPMDLVPEDIPLEILYEDANVIVVDKPQGMVVHPGSGNASHT
                     LANALVYHCAQVQALFPPGDLRPGIVHRLDKETSGVIIVAKNPSAHEMLAGQFRARSV
                     RKRYLALVRGRPKEEAGVIDQRIARDPRHRKRFTGVASGGRQATTRYRVVQTIDGYSV
                     LQLAPRTGRTHQLRVHLLHIGLPILGDPIYGTRDSRFPDATLMLHARSLTLLLPGESE
                     PRRFVAAVPERFYEVLRELYSSSPRNGL"
     gene            complement(1040..1423)
                     /locus_tag="VFH83_15520"
     CDS             complement(1040..1423)
                     /locus_tag="VFH83_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487836.1"
                     /translation="MKRSCRWLGILLLVAIVGPASTFAQERTPQPYTEDEFPQWVKDL
                     RRAEIVFVGSFPITLFLTLETYDTYRFVATGFNPTYTPWPLGSGAPYVNGETPWLAVS
                     ALSLSLVVAGIDFLIGRLNAPAPNH"
     gene            complement(1420..2142)
                     /locus_tag="VFH83_15525"
     CDS             complement(1420..2142)
                     /locus_tag="VFH83_15525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET6487837.1"
                     /translation="MVEPVLAPGAEALRKSLSAIQERIDRAARDAGRQPQEVRLMAVT
                     KTLPRQVVDSVIACGLSLFGENRVQEAEEKFLDLTESVELHLIGHLQRNKARAASGLF
                     RCVQSIDKLETAQALETRLSDRGRAMDVLLELNTSGEPSKSGFLAPDELLRCLEEILR
                     MPHLRPRGLMTVGPLTEDAGRVRSSFAQLRQLFDRVRSGGGPDGFDTLSMGMSADFET
                     AIAEGATLVRVGTALFGPRGPA"
     gene            complement(2136..2660)
                     /locus_tag="VFH83_15530"
     CDS             complement(2136..2660)
                     /locus_tag="VFH83_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET6487838.1"
                     /translation="MQSEGPTVSPAFRIAAVAVLAAVTTVFTLLIQVPSPARGYFNLG
                     DVAIAFTAYTFGPLTALISGGIGTALADLIGSYVQWAPITLVTHGLEGLVIALIFRLR
                     PSNPIVVILAGIAGVAVMVGGYLAGGILLTGIGPALAEAPGNVVQSAAGVIVGIPLSI
                     AVTRAYPPVRRFGW"
     gene            complement(2771..>3476)
                     /locus_tag="VFH83_15535"
     CDS             complement(2771..>3476)
                     /locus_tag="VFH83_15535"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HET6487839.1"
                     /translation="VPLCTCFFTAAPVWVLLPEVLVLVLVVLLALGPRELARPFAAIG
                     PVLLLIALLTPPFHRGGVAYLRIAGWPILTSEGVDWTVRLLVRFAGITLACVAVLRSI
                     DLDHLVLSLRWFGLRYDLCLVVVIAFRFIPSLGATWRNVLDAHRLRSSPEELQARRGR
                     SAQYLPALTSVLVEAVKGIPALAMALESRGFGRSNPRTSYLELKGGGALALDGVIGAA
                     LSTLLLVAAFARWPHP"
BASE COUNT          653 a         1144 c         1189 g          490 t
ORIGIN      
        1 cggatacggg cgggggctcc tgctggacat ggtccgcaag aagatcctct ccctggggga
       61 cgaggtgctg gtcttccccg gacacggtcc gccgtccaag atcgggatcg agaagctcta
      121 caacccgttt ctgggcgagg agctgtagag ctctctcagc acctcgtaga agcgttcggg
      181 aacggcagcg acgaaccgcc gaggctccga ctcgccgggc aggaggaggg tgaggctccg
      241 cgcgtgcagc atcagcgtgg cgtccgggaa ccgggagtcc cgggtcccat agatgggatc
      301 gcccaggatg ggaaggccga tgtgaagcag atgcacccga agctggtgag tccggcccgt
      361 ccggggcgcg agctgcagga ccgagtagcc atcgatggtc tggacgaccc ggtagcgggt
      421 cgtcgcctgc cgcccgcccg aggccacacc cgtgaaccgc tttcggtgcc tgggatcccg
      481 ggcgatcctc tggtcgatca cacctgcctc ttctttgggc cgccccctca ccagtgccag
      541 gtagcgcttt cggaccgacc gggccctgaa ttggccggca agcatctcgt gagccgatgg
      601 attcttcgcc acgatgatga ccccggaggt ttccttgtcc aggcgatgca caatgcccgg
      661 acggaggtct ccgggcggaa agagagcctg gacctgcgcg cagtggtaca cgagagcatt
      721 ggcaagcgtg tgggaagcgt tgccgctacc cggatggacg accattccct gcggcttgtc
      781 caccacgatc acgttggcat cctcatagag gatctccagc ggaatatcct cggggaccag
      841 gtccatcggc ggagcttccg accagcgtac ccgcaccacg tcgcccaact tcaggcgctt
      901 gctgagacgg gccggtttcc cgttgaccag tgcctcctgg actctgcccc tcgcctggct
      961 gcgggagaat aggccgaggc cctccgagag gaacaggtcg agccgcatcc cggccagctc
     1021 gggccggtcc accgcggcct cagtggtttg gggcgggagc attaaggcgg cctatcagga
     1081 agtcgatgcc ggccaccacg agggagaggg acaacgcgga gacggccagc cacggcgtct
     1141 ccccgttcac atagggcgcg ccggagccaa ggggccaggg cgtgtacgtt ggattgaatc
     1201 cggtagccac gaaccggtag gtgtcatacg tctccagagt caggaacaga gtgatgggga
     1261 acgagccgac aaagacgatc tccgcccggc ggaggtcctt cacccactgg ggaaactcat
     1321 cctccgtgta cggctggggc gtccgctcct gcgcgaacgt ggacgccggc cctacgatgg
     1381 cgacgagcaa caggatgccg agccagcgac aggaccgctt catgccggtc cccgaggacc
     1441 gaagagcgcc gtccctaccc ggacgagcgt ggccccttcc gcgatggctg tctcgaagtc
     1501 cgccgacatc cccatggaga gagtatcgaa gccgtcgggt ccgccgccgg accggacacg
     1561 atcgaagagt tgccgtagct gggcaaagga cgagcgcaca cgccccgcgt cctctgtaag
     1621 gggcccgacg gtcatgaggc cccgcggcct gaggtgcggc atccgcaata tctcctcgag
     1681 gcatcgaagg agctcgtccg gcgccaggaa gccggatttg ctgggctctc cgctggtgtt
     1741 cagctccagg aggacatcca tggcgcggcc gcggtccgac aaccgcgtct ccagggcctg
     1801 cgccgtctcc agcttgtcga tggactgcac gcagcgaaag agccccgacg cggcgcgggc
     1861 cttgttccgc tgaaggtggc cgataagatg gagctccacc gactccgtga gatccaggaa
     1921 cttctcttcc gcctcctgga cccggttctc cccgaagaga gacaacccgc atgcaatgac
     1981 cgagtcgacg acctgccgtg ggagggtctt ggtcacggcc atcaggcgga cctcctgagg
     2041 ctgcctcccc gcatcccgcg cggctcgatc gatacgctcc tggattgcgg agagggactt
     2101 gcggagcgcc tccgcgcccg gggcgagcac gggctctacc acccgaatct ccgcacgggc
     2161 ggatacgctc gggtcacggc gatggagagc gggatgccca cgatcactcc cgcggcgctc
     2221 tgtacgacat tcccaggcgc ctcggcgagc gccggcccga tccccgtgag gaggattccg
     2281 cccgccaggt acccgcccac catcacggcc actccagcga tgcccgccag gatcaccacg
     2341 atggggttcg aggggcgcag acggaagatg agggcgatca caaggccctc gagcccgtgc
     2401 gtcaccaggg tgatgggagc ccactgcacg taggacccga tcagatccgc cagcgcagtg
     2461 ccgatcccgc ccgagatgag ggccgtgagc ggaccgaacg tgtacgccgt gaacgcgatg
     2521 gcaacgtcgc cgaggttgaa ataccctcgg gcgggagacg ggacctggat cagaagcgtg
     2581 aagaccgtgg tcacggcggc gagcacggcg accgcggcga tccgaaacgc gggggagacg
     2641 gtagggccct cgctctgcat ttcgcgtcct cctggctgag tataggcaaa accaggatcg
     2701 ctgaaaagcg cggatctgcc gcaaggggtc gaagccgcgg gcagcacgcg cccgttgggc
     2761 gccggggcgc ctaagggtgc ggccatcgcg cgaacgcggc gaccaggagg agcgtgctga
     2821 gagctgcccc aatgacgcca tccagggcca acgcgccacc acccttcagc tcaagatagg
     2881 aggttctggg gttcgacctc ccgaatcctc ggctctccag agccatcgcc agcgcgggga
     2941 tgcccttgac cgcctctacc aggacggagg tcagggccgg gagatactgc gccgatcggc
     3001 cccgccgggc ctggagctcc tcgggactgg agcgcagccg gtgcgcgtcc agcacgttcc
     3061 gccaggtggc tcccagggat gggatgaacc ggaaggcgat gaccaccacc aggcacaggt
     3121 cgtagcgaag gccaaaccac cggaggctga gaaccaggtg atcgaggtcg atgctcctca
     3181 agacggcgac gcaggccagg gtgataccgg cgaagcgcac caggagcctc acggtccagt
     3241 ccacgccctc cgaggtaagg atgggccagc cggcaatcct gagatacgcg accccgcccc
     3301 ggtgaaacgg cggcgtcagg agcgcgatca ggaggaggac gggcccgatg gcggcaaacg
     3361 gccgcgccag ctctcgggga ccgagggcaa ggagcaccac gagcaccagc accaggacct
     3421 cggggagaag gacccacacc ggagctgccg tgaagaagca ggtgcagagc gggacg
//
LOCUS       DASRZS010000641         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2272799, whole genome shotgun sequence.
ACCESSION   DASRZS010000641 DASRZS010000000
VERSION     DASRZS010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2272799"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VFH83_15540"
     CDS             <1..741
                     /locus_tag="VFH83_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET6487840.1"
                     /translation="RGQYWMCQKHDIYGFKKSRAEGSWAQYMLYPALAINHKVPKSIP
                     DDQAALIEPLACAIHAVERGNIQLGDVVVIAGMGPIGLGMVSVARLKSPGMLIALDMK
                     PHRLELARTMGADLALDVSKQDAIAEVLKLTEGYGCDVYIEATGAGPAVVQGLQMIRK
                     LGTFVEFSVHGGPVAVDWSVIGDQKELTINGAHLGPYCYPKAINYLAAGTVNGAALVT
                     HKLPLEKFLDGIEIAHTGDRSSKVVLVP"
     gene            778..1329
                     /locus_tag="VFH83_15545"
     CDS             778..1329
                     /locus_tag="VFH83_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487841.1"
                     /translation="MTARALRAGTVLAALTLLVLGCQALPARFDVSPPVYQDDSVRIG
                     YRIGSQVYYIQIENRTDSEMTLDLNRCSIVSVTGETRSLRVSAGDTHIPPRSRITLRS
                     TQAALFGTDIDAMFTMPRLKLDGPPTEEAYLKRFVGSKIRLVIPITTAYLGTEHVYDI
                     QLSLDGVEHQGLPLPYQAEMDPP"
     gene            1398..>2464
                     /locus_tag="VFH83_15550"
     CDS             1398..>2464
                     /locus_tag="VFH83_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6487842.1"
                     /translation="MQRYLAIVCGLLVVASVFAGGTKEAAGAAVPSAPGPFGKYDPGL
                     TVNTVAVVSDALKFVPGEDINHNMWVKLFKDQMNLSFNYEWTAATQDQYDSKLNVNIA
                     AGNIPDVFDVNKTQLARLVQAGLINADLKPVYDKYATDLLKTFITQEGPYTLSSATFN
                     GKLAATPQSASSMDWANIMWLRQDWLDKLHLKAPTTYDDFVKMVNAFTTQDPDGTGAS
                     DYVGIALSKELFNMGEADFVGFANMFHAYPTIWVKDSSGQLVYGSYQPAMKAALLAAQ
                     KLYAAGKFDKEFAVKDNTKEAELISAGKTGIEFGQQWNPLWPLQSSVDNNPKADWTPY
                     PVPSVDSTPAMLQEQLGTSDY"
BASE COUNT          494 a          778 c          781 g          411 t
ORIGIN      
        1 cgcgggcagt actggatgtg ccagaagcac gacatctacg ggttcaagaa gagccgcgcg
       61 gaaggatcct gggcgcagta catgctctat cccgccctgg cgatcaacca caaggttccc
      121 aagagcatcc ccgacgatca ggcggccctg atcgagccgc tggcgtgcgc catccacgcc
      181 gtggaacgag ggaacatcca gctcggtgac gtcgtggtga tcgcgggcat gggccccatc
      241 ggattgggca tggtttcggt ggcgcgtctg aagagccccg gcatgctgat cgccctggac
      301 atgaaaccgc atcggctgga gctcgcccgc accatgggcg ccgatcttgc cctggacgtg
      361 agcaagcagg acgccatcgc ggaggtcctg aagctgaccg aggggtacgg gtgcgacgtg
      421 tatatcgagg ccaccggggc cggtccggcc gtggtgcagg gactccagat gatccgcaag
      481 ctgggcacct tcgtcgagtt cagcgtccac ggagggcccg tggccgtgga ctggtccgtg
      541 atcggagacc agaaggagct cacgatcaac ggcgcgcacc tgggacccta ctgctatccc
      601 aaggcgatca actacctggc agcggggacc gtgaacggcg cggcgctggt gacccacaag
      661 ctgcctctcg agaagttcct cgacggcatc gagatcgcgc atacgggaga ccgctcgagc
      721 aaggtcgttc tggtgccgta gggcctgcgc gacgggccat ccgcgggagg cgatcgggtg
      781 acggcacggg cgcttcgggc gggcacggtt ctggctgcgc tgacactcct ggtccttggg
      841 tgccaggcgc tgcccgcccg gttcgacgtt tcgcctccgg tataccagga cgacagtgtc
      901 cggatcggtt atcggatcgg gagccaggtc tactacatcc agatcgagaa ccggaccgac
      961 agcgagatga ccctggatct caatcgctgc tcgatcgtct ccgtcaccgg tgagacgagg
     1021 tctctgcgcg tgagcgccgg ggatacgcac atcccgccaa ggtcccggat cacgctccgg
     1081 tcgacccagg ccgccctctt cggcacggac atcgacgcga tgttcaccat gccgaggttg
     1141 aagctggacg gccccccgac cgaagaggcg tacctgaagc gcttcgtggg atcgaagata
     1201 cgactggtca tccccatcac cacggcgtat ctggggacgg agcacgtgta cgacatccag
     1261 ctctccctgg acggcgtgga gcatcaggga cttccgctgc cgtaccaggc ggagatggac
     1321 ccgccgtgaa aaccttttct tgacaaatcg agtgcagggc tcctacaata ccccgccact
     1381 tcaaaaggag gtgcggaatg cagagatacc ttgcaatcgt gtgcgggttg ctggtcgtgg
     1441 cgagcgtgtt cgccggcggc accaaggaag cagcgggcgc tgcggttcct tctgcgccgg
     1501 gtccgttcgg caagtacgac cccgggctca cggtcaatac cgtcgcggtg gtgagcgacg
     1561 ccctgaagtt cgtgccggga gaagatatca atcacaacat gtgggtcaag ctgttcaagg
     1621 accagatgaa cctctcgttc aattacgagt ggaccgcggc cacccaggac caatatgaca
     1681 gcaagctgaa cgtgaacatc gcggccggca acatcccgga cgttttcgac gtgaacaaga
     1741 cccagcttgc gcgtctggtc caggccgggc tcatcaacgc ggacctgaag cctgtctatg
     1801 acaagtatgc cacggacctg ctcaagacct tcatcacgca ggaaggcccc tatacgctga
     1861 gctccgccac cttcaacggc aagctggccg caacgcctca gtccgcctcc tcgatggact
     1921 gggccaatat catgtggctg cgccaggact ggctggacaa gctgcacctg aaggcgccca
     1981 cgacctacga tgacttcgta aagatggtca acgcgttcac gacccaggac cccgacggga
     2041 ccggcgccag cgactatgtc gggatcgcgc tctccaagga gcttttcaac atgggtgagg
     2101 ctgacttcgt cggctttgcc aacatgttcc atgcctatcc cacgatctgg gtgaaggact
     2161 cgagcggcca gctcgtctat ggcagctacc agccggccat gaaggcggcc ctcctggcgg
     2221 cgcagaagct gtacgccgcg ggcaagttcg acaaagagtt cgcggtgaag gacaacacca
     2281 aggaagccga gctcatctcc gcgggtaaga ccggcatcga gttcggccag cagtggaacc
     2341 ccctgtggcc tctgcagagc agcgtcgaca acaaccccaa ggcggactgg accccgtacc
     2401 cggtcccctc ggtggacagc acgccggcaa tgctccagga gcagctggga acctcggact
     2461 acta
//
LOCUS       DASRZS010000642         5931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2273435, whole genome shotgun sequence.
ACCESSION   DASRZS010000642 DASRZS010000000
VERSION     DASRZS010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2273435"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /gene="deoA"
                     /locus_tag="VFH83_15555"
     CDS             complement(<1..220)
                     /gene="deoA"
                     /locus_tag="VFH83_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375318.1"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HET6487843.1"
                     /translation="MRAVDLIVKKRDGGTLAPQEIDFLVRGFSAGEIPDYQFSALLMA
                     IVLRGMDTEETAQLTRSMIGSGETIDLSA"
     gene            complement(217..774)
                     /gene="thpR"
                     /locus_tag="VFH83_15560"
     CDS             complement(217..774)
                     /gene="thpR"
                     /locus_tag="VFH83_15560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET6487844.1"
                     /translation="MRVFAALPLPAAAIASILESLEPLRQRHPRLRWVNADAMHVTLH
                     FFGEVSESGVQRLRAVFERPVMRRRPLRVRLASFGQFPPQGRARVIWVGMDDGVDQMR
                     EYWQQLELLIAAEGWERDPRGFSPHITMARVGSSFLDPEWGTTIHAPRLEFELTECVL
                     YESVLERSGPRYLPLARAAFEERPS"
     gene            complement(771..1526)
                     /gene="recO"
                     /locus_tag="VFH83_15565"
     CDS             complement(771..1526)
                     /gene="recO"
                     /locus_tag="VFH83_15565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET6487845.1"
                     /translation="MYRSISTRAIVIRRERLGEFHKSLLLLTSDLGLIWATAYGAYKM
                     HSALRLGSEPFTASRALLYHNPVKNTYKVMELEIIESFPGLQADLARITAASLWAEVV
                     QKSYGAGETTDTLFRLLVEGLRLLQESDPRLEPYLTIQFLWRFLTLAGYLPDLGRCER
                     CSLPLDPSARAFYSASDNAVVCAACGGERGSLLPAGALRYLEASRHGSIRDASQVRLE
                     AASLGALRDVLPRMVQGIFEGQLKSLPSLGIRA"
     gene            1649..3988
                     /gene="lon"
                     /locus_tag="VFH83_15570"
     CDS             1649..3988
                     /gene="lon"
                     /locus_tag="VFH83_15570"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET6487846.1"
                     /translation="MSDHSIVPADQILPARLFVIPVRGKPVFPGILTPLMLPSAQDAE
                     TVEKAIAADSFIGLVLTKTEDVSQVTAEDLLPVGTAAKIVRRINLPDGGVNIFVTTLK
                     RFQVKRVLQAMPPISVVPVYLEDENPETAEVKALTRALISEMKQVSENNPLFSEEMRL
                     SMVNIDQPGKIADFITSILNIDRAEQQRILEMTNVQARMEHVLIFIKKEQELLKIQKK
                     ITEQLNEKISKSQREYFLKEELKAIKSELGMAVDAKSSEYQRFKEAVEKLQLQGEVKE
                     QVEQELEKFSLMEPASPEFTVTRNYLETIVALPWNSDSPESLDLEKAKAILNEDHYGL
                     EDVKGRIVEYLAVRKLKKDTKGSIVCLVGPPGVGKTSVGKSIARALGRQFFRFSVGGM
                     RDEAEIKGHRRTYIGAMPGKIIQGLKIVKVRNPVYMIDEIDKLGASFQGDPSSALLEV
                     LDPEQNVAFRDHYLDLPFDISHILFVTTANTLDTIPAPLLDRMEVIRLSGYIDREKVQ
                     IARSYLIPRSIERAGLPKGSVKYDNGALIAISEGYAREAGLRNFEKALDRIHRKIALK
                     TLDGGVKLPISMKKDCLADYLGKPVFLDEEMAQITKPGMATGLAWTPLGGAVLTIEAV
                     ANAGKEGLKLTGKLGEVMQESASIAYSYVRHASAEQGVGPDFWEKNLIHLHVPAGATP
                     KDGPSAGITLASALLSLATGRKIRKALAMTGELSLVGRVLPIGGLKEKVIAARRNHIK
                     HVLYPEGNQRDLDEIPEPVREGITFTPVASMDEVIKAIF"
     gene            complement(4002..4583)
                     /locus_tag="VFH83_15575"
     CDS             complement(4002..4583)
                     /locus_tag="VFH83_15575"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET6487847.1"
                     /translation="MVLSIPLPETLAAVTDTLSAGGVTILPCDTIYGFVGVAPETEGR
                     IRAIKGRGEDKPFLQLLPNLDWLSRLTRQEIPTRFAARWPGPLTLVVQARSGGTVAVR
                     LPDSAFLRSVLAAVGKPLYSTSVNLAGSPPLHAIEEIIRQFESRVDLVVDAGVLPSAA
                     ASTIVDVTSRPYRILRQGALRIPDEDLEAGPAG"
     gene            complement(4573..5010)
                     /locus_tag="VFH83_15580"
     CDS             complement(4573..5010)
                     /locus_tag="VFH83_15580"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET6487848.1"
                     /translation="MRRILLVLILCFSIASLLIYLLGDSGLLAYARLSRYHRNLAANV
                     ENLRQRNTELQSELTGLQKGRERTTILARSIDLYTTGDRVIQIEGMGRSRRTVAVGDL
                     LSYNPPAETRSAAFKAGALLLAVVLLAGVPFARRWMRRREHGT"
     gene            5197..>5931
                     /locus_tag="VFH83_15585"
     CDS             5197..>5931
                     /locus_tag="VFH83_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487849.1"
                     /translation="MARERQGSSPGEKKLEQYGVWVKVRPREVTTTLEAEDSFELSDL
                     EARSEARPASAKEGALTTEEEELLDELEGELGPGKTAQPILIPDEEPLLSEAEELPDI
                     ESITESKPAASARGAAAVEEEIPQLEDTDLSIEPIEGPPEAPAAREEVEVALSESIDV
                     KDHFEDLETLETELTSVRNAVSSPRANSSVEILARIEEELRSIRSDLTQLRTELNGLS
                     KTVTSPTDQQKPQGSKPQGFFDEEEDE"
BASE COUNT         1199 a         1854 c         1942 g          936 t
ORIGIN      
        1 ccgcggagag gtcgatggtc tcaccggagc cgatcatgga tcgagtgagc tgcgccgtct
       61 cctccgtgtc cattccgcgc aggacgatgg ccatcagaag cgcggaaaac tgatagtcgg
      121 ggatctcgcc cgcgctgaag ccccggacga gaaagtcgat ctcctgcgga gcgagcgtgc
      181 cgccgtcccg cttcttgacg atgagatcca ccgccctcac gaggggcgct cctcgaaggc
      241 ggcgcgcgca agcggcaggt acctcggacc gctgcgctcc aggacggatt cgtagaggac
      301 gcattccgtg agctcgaact cgagccgggg cgcgtggatg gtggtccccc actcgggatc
      361 caggaaggag ctgccgaccc gggccatcgt gatgtgtggc gaaaacccgc gcgggtccct
      421 ctcccagcct tccgccgcga tcaggagctc gagctgttgc cagtactccc gcatctggtc
      481 gaccccatcg tccatcccta cccagatgac ccgtgcccga ccctggggag ggaactggcc
      541 gaaggaggcc aaccggactc gcaacggacg cctccgcatg accggccgct cgaagacggc
      601 gcgcagacgt tggacgccgg actccgatac ctccccgaag aagtgcagcg tgacatgcat
      661 ggcgtccgcg ttcacccatc ggagccgggg atgcctctga cgcaagggct ccagggattc
      721 gaggatggag gcgatggctg ccgcgggcag gggcagtgcc gcgaagaccc tcacgcccga
      781 atccccaggg acggcaggct cttgagctgc ccttcgaaga tcccctggac catccgagga
      841 aggacatccc gcagggcgcc gaggctggcc gcctcgagcc tgacctggga ggcatcccga
      901 atgctgccat ggcggctcgc ctcgaggtac cgcagggcgc ccgcaggcag gagggacccc
      961 cgttctcccc cgcacgccgc gcataccacg gcgttgtcgg aggcggaata aaaggcgcgg
     1021 gcggacgggt ccagcgggag ggagcaccgt tcgcaccgcc ccaggtccgg gagatacccg
     1081 gccagggtca gaaaccgcca caggaactgg atcgtcaggt atggctcgag tcgggggtcg
     1141 ctctcctgga gcagccgcag cccctcgacc aggagccgga aaagcgtatc ggtcgtctcc
     1201 ccggcgccgt acgacttctg gaccacttcg gcccagagac ttgcagccgt gatcctggcg
     1261 agatccgcct gcaatccggg gaagctctcg atgatctcca gctccatcac cttgtaggtg
     1321 ttcttgacgg ggttgtggta cagcagcgcc cgggatgcgg tgaacggctc gctgccgaga
     1381 cgcagggcgc tgtgcatctt gtacgcgccg taggcggtcg cccaaatgag ccccaggtcc
     1441 gacgtaagga ggagcaggct cttgtgaaac tcgccaagcc gttccctgcg tatgacaatg
     1501 gctcgcgtgg agatgcttcg gtacattcgc gctcgctgtc tcgattggat aatagtcgag
     1561 agctgcgacc cgtggcaagg cgggaaatcc cgttcttgac ctgccatccc gggggtacta
     1621 ccttcaacct tacaacggag acaacgaaat gagtgatcac agtatcgtgc ctgcggatca
     1681 gattttgcct gcccgcctgt tcgtcatccc tgttcgcggg aagccggtgt tccctggcat
     1741 cctcactccc ctcatgctgc cttcggcgca ggacgccgaa accgtggaga aggcgatcgc
     1801 cgcggacagc ttcatcggac tggttctcac gaagacagag gacgtctccc aggtcaccgc
     1861 ggaagacctt ctcccggtcg gcacggccgc caagatcgtc cgtcggatca acctcccgga
     1921 cggcggggtc aacatcttcg tcaccacgct caagcgtttc caggtcaaga gagtcctgca
     1981 ggcgatgccg ccgatcagcg tcgtcccggt gtatctggag gacgagaacc cggagaccgc
     2041 cgaggtcaag gcgctgaccc gcgccctcat cagtgagatg aagcaggtgt cggagaacaa
     2101 tcccctcttc tcggaggaga tgcggctgtc catggtcaac atcgaccagc ccggaaagat
     2161 cgccgacttc atcacttcca tcctcaacat cgatcgggcc gagcagcagc ggatcctgga
     2221 aatgacgaac gtccaggcgc gcatggagca cgtcctgatc ttcatcaaga aggagcagga
     2281 gctgctcaag atccagaaga agatcacgga gcagctcaac gagaagatct ccaagagcca
     2341 acgggagtac ttcctcaagg aggagctgaa ggccatcaag tccgagctgg ggatggcggt
     2401 agatgcgaag agcagcgaat accagcggtt caaggaggcc gtggagaagc tccagctcca
     2461 gggagaggtg aaggagcagg tggagcagga gctggagaag ttctcgctca tggagccggc
     2521 ctctccggag ttcaccgtca cgcggaacta cctggagacc atcgtagccc tgccgtggaa
     2581 cagcgactcg ccggagtccc tcgacctgga gaaggccaag gccattctga acgaggacca
     2641 ttacgggctt gaggacgtga agggacggat tgtcgagtac cttgccgttc gcaagctgaa
     2701 gaaggacacg aagggctcga tcgtgtgtct ggtgggtccc ccgggggtgg gaaagacatc
     2761 cgttggcaag tccatcgccc gcgcgctggg ccggcagttc ttccgatttt cggtcggcgg
     2821 catgcgggac gaagcggaga tcaagggcca ccgtcggacc tacatcggcg ccatgccggg
     2881 gaagatcatc cagggcctga aaatcgtgaa ggttcgaaac cccgtctaca tgatcgacga
     2941 gatcgacaag ctgggggctt ccttccaggg agatccgtct tccgcgctcc tggaggtcct
     3001 ggaccccgag cagaacgtcg cctttcggga ccactacctg gacctcccct tcgacatctc
     3061 ccacatcctc ttcgtgacca cggccaacac cctggatacc atcccggccc ccctcctgga
     3121 tcgcatggag gtgatccgcc tctcgggata catcgatcgg gagaaagtgc agattgcccg
     3181 ctcgtatctc atccccaggt cgatcgagcg cgccggcctt cccaagggga gcgtgaaata
     3241 cgacaacggc gccctcatcg cgatctccga gggctacgcc cgggaggcgg gcctgcggaa
     3301 cttcgagaag gcgctggacc ggatccatcg caagatcgcg ctcaagacgt tggacggggg
     3361 cgtgaagctc cctatctcaa tgaagaagga ctgcctggcg gactatctgg gcaagccggt
     3421 gttcctggat gaagagatgg cgcagatcac caagccgggc atggccacag gacttgcatg
     3481 gacgcccctg gggggcgcgg tgctgaccat cgaagcggtg gcgaacgccg gtaaggaagg
     3541 gctcaagctg accggcaagc tcggggaggt gatgcaggag tcggcgagca tcgcgtactc
     3601 ctacgtccgc cacgcctccg ccgaacaggg cgttggaccg gacttctggg aaaagaacct
     3661 gatccacctc cacgttccgg ccggcgccac tcccaaggac ggtccgtcgg ccggcatcac
     3721 cctggcctcc gcgctcctct ccttggcaac cggccgcaaa atccgcaagg cgcttgcgat
     3781 gaccggggag ctctcgctgg tcggacgggt cctccccatc ggtgggttga aggagaaggt
     3841 catagcggca cgccgaaacc acatcaaaca tgtcctctat ccggaaggga accagcgcga
     3901 cctggacgag atccctgaac cggtccggga ggggatcacg ttcactccgg tggcatcgat
     3961 ggacgaggtg atcaaagcga tcttctagct gcgaggcacc gtcagccggc gggaccggcc
     4021 tccagatcct catccgggat tctgagcgcc ccctgccgca ggatccggta cgggcgggag
     4081 gtaacgtcca cgatggtgga tgcagccgcg gacggaagga cgcctgcgtc cacgacgagg
     4141 tcgacgcgcg actcgaattg gcggatgatc tcctcgatcg cgtgcagggg tggggagccc
     4201 gcgaggttga cgctcgtgga gtacagcggc ttccccaccg ccgccagcac cgaccgcaga
     4261 aacgcggagt ccgggagccg aaccgcgacg gtcccacccg atcgggcctg gaccacgagc
     4321 gtgagcggcc cgggccatcg cgcggcgaac cgtgtcggga tctcctgacg ggtgagccgg
     4381 gaaagccagt cgaggttcgg cagaagctgg agaaagggtt tgtcctctcc cctgcccttg
     4441 atggcgcgga tgcggccctc ggtctccggc gccacgccca cgaatccgta gatggtgtcg
     4501 cagggaagga tggttacgcc gccggcggaa agggtatcgg tcaccgccgc gagagtttcc
     4561 gggagcggga tgctaagtac catgctccct gcgccgcatc caacgccggg cgaacggcac
     4621 gcccgcgagc agcaccacgg ccagcagaag ggcccccgcc ttgaaggccg cgcttcgggt
     4681 ctcggcggga gggttgtagg aaagcaagtc tcccacggcc accgtccgcc tcgagcgtcc
     4741 cattccctcg atctgaatga cgcggtcccc ggtggtatag agatcgatgg agcgagccag
     4801 gatggtcgtc cgctcgcgtc ccttctgcag accggtgagc tcggattgga gctcggtgtt
     4861 cctctggcgc agattctcca cgttcgccgc caggttccga tggtatcggc tcaaccgcgc
     4921 ataggcaagg agaccggaat cgccgaggag atagatcaag agggaggcta tggagaaaca
     4981 gaggatgagc acgaggagga ttcgccgcat ggccttctgc tccagataac ggcctggctt
     5041 ggccaaaaga tgagcgcggg gcccttcaag cggcccatgc cattgacaac ccgaaaagtg
     5101 ctggactaca ttaccggagc gtgaaatgcg tgcaagtgca agtctttgcg tgccgataag
     5161 tgaggatggc gtgacacgcg tcctcaggag atgggaatgg caagggaaag acagggcagc
     5221 tctccaggtg agaagaaact cgagcagtac ggcgtctggg tcaaggttcg ccctcgcgag
     5281 gtaacgacta ccctggaggc cgaagactcc ttcgagctta gcgacctgga agcccggagt
     5341 gaggcgcggc cggcgtctgc caaggaaggt gccctgacca ccgaggaaga ggagctcctg
     5401 gacgagctgg agggggagct gggacccggg aagaccgcgc aaccgatcct tatcccggac
     5461 gaggaacccc tgctgtcaga ggccgaggag cttccagaca tcgagtccat cacggaatcg
     5521 aagccggcag cctcggccag aggcgccgcg gccgtcgaag aggagattcc ccagctcgag
     5581 gacaccgacc tctcgatcga gccgatcgag ggcccacccg aagctccagc cgcccgtgag
     5641 gaggtggagg tcgccctctc cgagtccata gacgtcaaag atcatttcga ggatctggag
     5701 actctggaga ccgagctcac gagcgtcaga aacgcggtct cttctccacg agccaactcg
     5761 tcggtcgaga tcctggcgag gatcgaggag gagctgcgat ccattcgcag tgacctcacc
     5821 caactgcgca cggagctgaa cgggctgagc aagacggtga cgtcccccac ggaccagcag
     5881 aagccgcagg gctcgaagcc gcagggcttc ttcgacgaag aggaggacga g
//
LOCUS       DASRZS010000643         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4977584, whole genome shotgun sequence.
ACCESSION   DASRZS010000643 DASRZS010000000
VERSION     DASRZS010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4977584"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFH83_15590"
     CDS             complement(<1..632)
                     /locus_tag="VFH83_15590"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HET6487850.1"
                     /translation="MLAVVASHAPFGMEGELVSVEVDIRRGLPGIDVVGLPDGAVRES
                     KERVRVAIRNSGFAFPLDRILVSLAPAGVRKEGASFDLPIALGILAASGQIAADGLVG
                     PMVLGELNLGGTVRPVNGVLSAVGCGTEAGIKTFLVPIENVREAMALGAGSVLGIRSL
                     REAALALSGALPAERADLAPPPPAAPDCSDLDELEDIKGHDSLKRALQVAA"
     gene            complement(661..1119)
                     /gene="rnhA"
                     /locus_tag="VFH83_15595"
     CDS             complement(661..1119)
                     /gene="rnhA"
                     /locus_tag="VFH83_15595"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HET6487851.1"
                     /translation="MAVELWTDGGCVGNPGPGGWAFVLRWPEGEIAQSGFEVETTNNR
                     MEMTAVLQGLRHVTANADLRRVRLLVVTDSQYVHKGMTQWMASWERSGWKTSNKKPVK
                     NQDLWMALAEAARGLDLEWRWVLGHAGNEMNERCHDLVEQAIAKGGKRPA"
     gene            complement(1162..>2321)
                     /locus_tag="VFH83_15600"
     CDS             complement(1162..>2321)
                     /locus_tag="VFH83_15600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6487852.1"
                     /translation="LATAYYRSRLAADRRRIQWLLAGIAIALPPYFFFDQLPIILAGS
                     GMRLSLGSFSQLFLSFIPVFVLIGLTRENRFDPRGFLARYVSYVVLFGCMVALFAVLY
                     LPLRGLLSTGYRLSSPASDLVASAAIVVLLACLRILFENLARRWLRRQSLAPRHSGRT
                     EPDQPEAEEALRREYGLAARRQAVGEQRALIRGTLQRLREPLRRIQAGASAGDREVRE
                     AAAQVGGFLAALESLAGSEAPIAATARPRTMALRAMDRVAARFPSVRFEAAGDGTEPV
                     SCYPEEVLRALIAVLENAAESQAEGGSVTVRLDRTDSAVSMEVQDDGPGFGVLARRRL
                     FTPFYTTKPGHQGLGLYLARIAVERCGGALEVVEGEPGGARVRISLPIGCG"
BASE COUNT          414 a          812 c          737 g          358 t
ORIGIN      
        1 gcggcaacct ggagcgcgcg cttcagagag tcatggccct tgatgtcctc cagctcatcg
       61 aggtcggagc aatccggggc ggccggtggg ggcggggcca agtccgcgcg ctctgcggga
      121 agggcgccgg agagcgcgag cgccgcctcc cgcagggagc ggatccccag cacgctgccc
      181 gcccccaggg ccattgcctc tcggacattc tcgatgggga cgaggaaggt cttgatcccg
      241 gcctccgttc cgcaccccac cgccgacaga acgccgttca cgggtctcac cgtcccgccg
      301 aggttgagct ctcccagcac catgggaccg acgaggccgt ctgccgcgat ctggccagaa
      361 gccgcaagga ttcccaacgc aatgggcaag tcaaaggagg cgccttcctt gcggactccg
      421 gcaggcgcca gggacaccag gatccgatcc agtgggaagg cgaacccgct gtttcggatt
      481 gctacgcgga ctctctcttt ggactcccgt accgcgccgt ccggcagtcc gaccacatcg
      541 atgcccggca gcccccgacg gatgtccacc tcaaccgaga cgagctcccc ttccatgccg
      601 aacggcgcat ggcttgcaac gacggccagc attcttcctc cccgaatatc aatgggaagg
      661 tcaggccggc cgcttgcctc ccttggcgat cgcctgctcg accagatcgt ggcagcgttc
      721 gttcatctcg ttcccggcat ggcccaggac ccagcgccac tccagatcaa gtccccgagc
      781 cgcttccgcg agcgccatcc acaggtcctg gtttttcacg ggcttcttgt tgctggtctt
      841 ccatcccgag cgctcccagg acgccatcca ctgggtcatc cccttgtgca cgtactgcga
      901 atccgtcacg accagcaggc gcacgcggcg caggtcggcg ttcgccgtga cgtgccgcag
      961 cccttgcaga accgccgtca tctccatacg gttgttcgtc gtctcgactt cgaagcccga
     1021 ctgcgcgatc tccccttccg gccatcgaag gacaaacgcc cagccccccg ggccggggtt
     1081 cccgacgcag ccgccatccg tccaaagctc tactgccacc ttttctcctc ggagctgtgc
     1141 agaacaccgc gcgggatccg gtcatccgca gccgatgggg agagagattc gcaccctcgc
     1201 tccgcccggt tcgccctcca cgacctcaag ggcgccgccg catcgctcca cggcaatcct
     1261 ggcgaggtag agtcccagtc cctggtgtcc cggcttggtg gtgtagaatg gcgtgaagag
     1321 cctccttcgc gcaagcacgc cgaatccggg cccgtcgtcc tgaacctcca tgctgacggc
     1381 ggaatcggtt cggtccaggc gtacggtgac cgagccaccc tccgcttggg attccgcggc
     1441 attctccaat acggcaatca aggcccgcaa gacttcctca gggtagcagc tcaccggctc
     1501 ggtaccgtct cccgccgctt cgaaacgaac ggacgggaag cgcgcggcaa ctcggtccat
     1561 ggcccgaagc gccatagtgc ggggacgcgc ggttgccgcg atgggcgcct ccgagccggc
     1621 cagggactcc agcgccgcaa ggaagcctcc gacctgggcc gcggcctccc ggacttcccg
     1681 gtcccccgcg gacgcgccgg cctggatccg tctcaagggc tcgcgcaacc tttgaagggt
     1741 ccctcggatc agggcccgct gctctccaac ggcctgacgc cgcgctgcca gcccatattc
     1801 gcggcgcagt gcttcctcgg cctccggttg gtcgggctct gtccgtccgc tgtggcgcgg
     1861 ggccaggctc tggcgccgga gccatcgccg ggcaagattc tcgaacagga ttctcaggca
     1921 ggcgaggagc acgacgatgg ccgcgctggc caccagatcc gacgctgggg aggacagccg
     1981 gtaccccgtt gagaggaggc cgcgcagcgg gagatagagc acggcaaaca gcgccaccat
     2041 gcatccgaaa aggacgacgt acgagacata tcgggcaagg aagccgcgcg ggtcgaaccg
     2101 gttctcgcgg gtgagaccga tcagcacaaa gaccggtatg aaggagagga aaagctggga
     2161 gaaggaaccc agggacaatc gcatcccgga accggcaagg atgatcggga gctgatcgaa
     2221 gaagaagtag ggagggagcg cgatggcaat gccggcgagg agccactgga tccgtcggcg
     2281 atccgccgcc aggcgggagc gatagtaggc agtggcgaga a
//
LOCUS       DASRZS010000644         3574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3424688, whole genome shotgun sequence.
ACCESSION   DASRZS010000644 DASRZS010000000
VERSION     DASRZS010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3424688"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VFH83_15605"
     CDS             <1..446
                     /locus_tag="VFH83_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487853.1"
                     /translation="LDEPANGLDPAGIAEIRELLRGLSRQRGVTVFLSSHILGEIAQL
                     ADRIGIIHRGRLVEEVSEGSAGLGSWIEATVSDATRGAAVLTGSLGLSAVTVTGPRGL
                     RIEADHCQPRDVARAIVGAGLDLEKLCPVSEDLEARFMRLTGGEE"
     gene            443..1240
                     /locus_tag="VFH83_15610"
     CDS             443..1240
                     /locus_tag="VFH83_15610"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487854.1"
                     /translation="MTAMRSFTSTLSTEFLKLRRNRITWVTLAFYAVGPLAAALFMVI
                     IKDPEFARRAGLANQKAHLMVSTADWPSYLSLILEMAIMFGIVLIGAIIAYVFGREYT
                     EQTAKNMLALPVRREWFVSGKLIVTTVWFVALVAIVLTESILVGLLLGLPGFDGGLLL
                     ATIGNIAAVSGLALLLSPVFGWIAVATKGYLAPIGATMLALILANVMAQTGWGKWFPW
                     SILGLFLGVTGPRSETLSTGSYGVIAIVCLLGVLGTMVHMRRADNTQ"
     gene            1250..1960
                     /locus_tag="VFH83_15615"
     CDS             1250..1960
                     /locus_tag="VFH83_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487855.1"
                     /translation="METDIDAAARRARGYLFVDGFAEMAAGVVFVLMGSVMLLGGLIP
                     GESLLTQVVAAGLGILIVKAIGLLAAALAIWWLKDRFTYPRTGYVRHKRIRLSQILSL
                     VRNAFLVLALPLLALAAAFALVPSLRGAVASVPVWVPALVGIVWGAPCYWLGEWVGLR
                     RFRLLGLVTFVAGIVVGGALLLTGWSASGDAGQILGRTFVGVGLLTVTCGAAFLLSGL
                     VTFLRYRKENPLPYGQEP"
     gene            1957..2274
                     /locus_tag="VFH83_15620"
     CDS             1957..2274
                     /locus_tag="VFH83_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET6487856.1"
                     /translation="MTDRLPNLQDLDRVIHEPSRLLLTALLSTVESADFLFLLKQSGL
                     TKGNLSVHLTRLEEAGYLSVKKSFRGKIPHTEYALTPKGTSAFDRYRKSLGPILNARG
                     KQR"
     gene            2556..3353
                     /locus_tag="VFH83_15625"
     CDS             2556..3353
                     /locus_tag="VFH83_15625"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET6487857.1"
                     /translation="MAGLAALLAWSLFGGPGLLWGAGAAVLALLVTPRISPPAALRMY
                     GGRRLSAYQAPQILGLVQELSQRAGLENVPRVYYLPTGQVNALSVGTRKDSAIGVTEG
                     LLRSLSVRELAGVLAHEVAHITAGDLRVMGLARAARMATNALSFLGRFLLILNLPLIL
                     LGVTGLPWLFVLLLLAAPLVSSLLETALSRSREFAADRRAVSLTGDTAGFASALQRID
                     QASSLLRRMLGVPTRGSRLLDTHPANSARIQRLLAQEDRRAPRFLAA"
BASE COUNT          553 a         1134 c         1183 g          704 t
ORIGIN      
        1 tgctggacga gccggcaaac gggcttgacc ccgcggggat agccgagatc cgcgagctgc
       61 tgcgggggct ctcccgtcaa cgaggcgtga cggtatttct ctccagccac atccttggag
      121 agatcgccca gcttgcggac cgcatcggga tcatccaccg ggggcggttg gtggaggagg
      181 tctcggaagg ctccgccggg ctgggctcgt ggatagaggc gaccgtctcg gatgcgacgc
      241 ggggcgcggc ggtcctgacg ggcagccttg gactctccgc ggtcaccgtg accggaccgc
      301 gcgggctccg catcgaggcg gaccactgcc agcctcggga cgtggcccgc gccatcgtgg
      361 gagcgggcct ggatctggag aagctgtgcc cggtctccga ggacctcgag gcgcgtttca
      421 tgagattgac gggaggtgag gaatgacggc aatgcgatcg ttcaccagca ccctgagcac
      481 cgagttcctg aagctcaggc gaaacaggat tacgtgggtc accctggcgt tctacgccgt
      541 cggccccctg gccgcggcgc tgttcatggt catcatcaag gaccccgagt tcgcgcggcg
      601 ggcgggtctg gccaatcaga aggcgcacct catggtcagc accgcggact ggccgagcta
      661 tctttcgctc atcctcgaga tggccatcat gttcggcatc gtcctcatcg gtgccattat
      721 cgcgtacgta tttggccgcg agtacacgga acagacggcg aagaacatgc tggcgctgcc
      781 cgtgcgccgg gaatggttcg tatccggcaa gctcatcgtc accacggtct ggttcgtcgc
      841 tcttgtggca atcgtcctga ccgaatcgat cctggtcgga ctcctcctgg gcctgccggg
      901 cttcgatggc ggcctgctcc tggcaaccat cggaaacatc gcggcagtct cgggactggc
      961 actgctgctg agcccggtgt tcggatggat cgccgttgct accaaaggat acctggcccc
     1021 gattggggct accatgcttg ccctcattct tgctaacgtg atggcgcaga ccgggtgggg
     1081 gaaatggttc ccctggtcca tcctggggct cttccttggg gtgacgggac ccagaagcga
     1141 gaccctgagc acgggaagct acggggtgat cgcgattgtc tgcctgctcg gcgtcttggg
     1201 cacgatggtc cacatgaggc gggcggacaa cacccagtag gaaagggaca tggagacgga
     1261 tatcgatgcc gccgcaaggc gcgccagagg gtatctgttc gtcgacggtt tcgccgagat
     1321 ggctgccggc gtggtgttcg tcctgatggg gagtgtgatg ctcctgggag gcctcatccc
     1381 gggggagtcg ctcctgaccc aggtcgtcgc cgcaggactt gggatcctga tcgtcaaggc
     1441 gatcgggttg ctggcagccg cccttgccat ctggtggctc aaggatcgct tcacctaccc
     1501 ccgaacgggg tacgtccggc acaaaaggat ccgcctttct cagatccttt cgttggtccg
     1561 aaacgcgttt ctcgtgcttg cgcttcctct gcttgcgctg gcggcggcct tcgcccttgt
     1621 gccctcgctg cgtggcgccg tggcctccgt tcccgtctgg gtgcccgcgc tggtgggcat
     1681 cgtctgggga gcgccctgct actggctggg cgaatgggtg gggctgcggc gcttccgtct
     1741 tctgggtctg gtgacgttcg tcgcgggcat cgtggtgggc ggggcgctgc tgctgacggg
     1801 ttggtcggct tctggggatg ctggccagat cctgggccgg acgtttgtcg gcgtgggcct
     1861 cctgacggtg acctgcggcg ccgcctttct cctctcgggc ctcgtgacgt ttcttcgcta
     1921 tcgcaaggag aaccctttgc cctacgggca ggaaccatga cggaccgact gcccaacctc
     1981 caggatctgg accgagtcat tcacgagccg tcgcgcctgc tgctcacggc actcctgtcc
     2041 accgtggagt ccgcggattt cctctttctc ctcaagcaga gcgggctgac caaggggaat
     2101 ctctccgtcc acctcacgcg attggaggag gcgggatacc tcagcgtgaa gaagtcgttc
     2161 cgcggcaaga tccctcatac cgagtacgcg cttacaccga agggaacctc agcgttcgac
     2221 cggtatcgaa agagcctagg cccgatcttg aacgcgaggg gcaagcagcg gtaaacgagc
     2281 caggtccctg gcgattgctg ggtcatggcg acccccgcct tccagcggct gggtcaatag
     2341 gatacagggc gcacgccgtc ttccagcgca cttccccgcc tgccgcagcg cgagcgccta
     2401 ttgcttcgtt ctgaaagcaa ttgcgttcac acctctcttc gatcgtatgg cacgcttcat
     2461 gcatctttcg tgtcgccatg aagacgaaca gagttcccac gccacgcctt gcagggtggc
     2521 gatcagtgtt tcagagcgcc ttggtcatag gcagtatggc cgggcttgcg gcccttctgg
     2581 cctggtccct gttcggcggt ccgggcctgc tgtggggcgc cggggcggcc gtgctcgcgc
     2641 tcctggtgac ccctcgcatc tcgcctcccg ccgcgctcag gatgtatggg ggaaggcgac
     2701 tgtcggcgta tcaggcgccg cagatcctgg gcctcgtgca ggagctgtcc caacgcgccg
     2761 gactggagaa cgttccccgg gtttactacc tgccaacggg acaggtgaat gcgctttccg
     2821 taggaacccg caaggattcc gccattgggg tcaccgaggg cctgctgcgc tcgctttctg
     2881 tccgcgagct tgccggcgtt ctggcgcacg aggtcgcgca catcacagcc ggagacctgc
     2941 gagtcatggg tctggccaga gccgcccgaa tggccacgaa cgcgctatcg ttccttggac
     3001 gattcctgct gatcctgaat cttcccctca tcctccttgg cgtcacgggt ctgccttggc
     3061 tcttcgtcct gttgctgctg gccgcgccgc tggtcagcag tctgctggag acggccctct
     3121 ctcgttcccg ggagtttgcc gctgaccgcc gtgcggtgtc ccttaccggt gatacggctg
     3181 gcttcgcctc cgcgcttcag cggatcgacc aggcctcttc gctcctcaga cggatgctgg
     3241 gtgtgcccac ccgcggctcc cgactcctgg acacgcatcc cgccaacagc gccaggatcc
     3301 agcggctcct ggcgcaggag gaccgccggg ccccgcggtt cctggcggcg tgagccggag
     3361 gggcggcaga tcggccctct gccgagacaa ggccaattcc gcggcagagt caggcgcaga
     3421 gggacgaccg atgcgggaag caacctcacc catggtataa tccgcaggga cacaagagga
     3481 ggcagggctt agggaacagc gtctcgatga gccctccgct gacacgggcg gacggccgat
     3541 cggttgcatc gtattgaaaa cggaaaagga gaaa
//
LOCUS       DASRZS010000645        15919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5368150, whole genome shotgun sequence.
ACCESSION   DASRZS010000645 DASRZS010000000
VERSION     DASRZS010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 15919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15919
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5368150"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VFH83_15630"
     CDS             complement(<1..808)
                     /locus_tag="VFH83_15630"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6487858.1"
                     /translation="MQSSAVRLGVVGTGSVVREIYQYLYFRSQYSGGIRVEAVCDTSP
                     QALSAFADAWKIPENRRFTSYTEMIRRVELDAVAVNTPDGLHREPTLCALAAGLDVIL
                     PKPTADRVVDAHAMMEAVRASGRFLGVDFHKREDPVTKEARARVRSGIYGPLQSSVWY
                     MLDRLLVSDPNHEPRFFSSADFAEKNSPISFLTSHMVDTFLFITGLRPLEIRAVGYRQ
                     KLPSLSPIPVQGYDTVDTSVLFEGGSLAHIITGWALPNTANCLTVQSARLL"
     gene            984..2099
                     /locus_tag="VFH83_15635"
     CDS             984..2099
                     /locus_tag="VFH83_15635"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6487859.1"
                     /translation="MKRDRMSSRERFHATFEYGTPDRAFLFPDWCFNDTILRWRREGL
                     PPSQHFNAFFGFDRMATIPLNPGYTEDLDAVWPRPATRVVDRSLEWHVVENELGGRYR
                     AWLDREIGMSQWIDFPVRDRESWERFKKWLDPDQPSRYPEYWDDLVRCYRGRDYPLGI
                     TAGSFYGWLRDWVGMENLALWYYDCPDLVHEIVEYTADFVIRWTARALTDVPDIDFAS
                     IWEDMCMKTGPLISPELYRAFHVEPMKRVIRAFRDAGIRIITLDSDGMVDSLIPIWMD
                     AGIDVIYPIERASGCDPVRYRTQYGKKLRMYGGIDKRVLRDGMPKSAIEKEVGEKAEL
                     IRQGGYTPQIDHVIPPDIPFENFRYYVDLVQEACRFG"
     gene            complement(2143..3000)
                     /locus_tag="VFH83_15640"
     CDS             complement(2143..3000)
                     /locus_tag="VFH83_15640"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET6487860.1"
                     /translation="MASSAMSARAVALALAGCVALSAGSAWCDSDPAQGRAGWHLAWS
                     DEFDGPALDTSAWTAELGDGSPQNPGWGNAEGESYTDRPENLSIVPDGSSRVLRITAR
                     SERYGGRYYTSARIKTQGKRSMAFGLVEARIRLPSGRGLWPAFWMMGESITSAGWPAC
                     GEIDILEMRGGDDGTVLGTMHWRDASGGHAASIPGVARLPSGAFGDAYHVFGVEWSGT
                     TLTWYLDGIPYVTQSLTEADREAFRGRPFFILLNLAVGGRFLSNQIPPIGFSSASMDV
                     DWVRWYQKD"
     gene            complement(2982..4175)
                     /locus_tag="VFH83_15645"
     CDS             complement(2982..4175)
                     /locus_tag="VFH83_15645"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6487861.1"
                     /translation="MANNLNEINTSLVLQKLWLSGGISRAEISRQLGLGKATVTKIVA
                     DLMRRNLVTVARRGRSGPSGGRSPLHLAINGEYGCVLGVEMQTDFGKAVAIDLKGEVI
                     FSHSQPIEFRAADPAATFLSFLEDIQRRARKIRMPLIGVGVGLAGVVDAAEGVITKSN
                     PLDVHSPLPFRAAVAERVGVPVLIENDANCCCWGELAFRKTERHRNFCFVLGEFRKGR
                     TTGEEYWGIAVGLGFVLNGKVYRGEGFSAGEFQSILWKPSNHGQFSVSDDDSRRIKSD
                     PRLLSGVLRELASHIAFLVNTLNLKSVVIGGEMAAYREELSRILVEEIQGNWSYKDTV
                     DCSIEFAALGDLAVAYGAAGMFLEHFFSVPEVADSLTRRKSPRLNVPARGARKTVRTA
                     WLRRP"
     gene            complement(4165..4935)
                     /locus_tag="VFH83_15650"
     CDS             complement(4165..4935)
                     /locus_tag="VFH83_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET6487862.1"
                     /translation="MVCDHFTSAELDRTLWNVAVTGAVVNDEQQAYIDSPTTVRCAPG
                     READADCDGALILQPRYSQGYLTAEDRRFDFVSGRIDTRGKLSLRHGTVSARIKLPAG
                     QGVWPAFWALGNGSWPEAGEIDIMENVGDPEWVNAALHGPGYAGETPLVNRRYFSGRD
                     ATAWRVYSVACGADSIVFSVDDEVIYRVLRPMVEHYGRWVFDDPKYLILNVALGGNYP
                     LKTNGIRSPYPGLPASTVQRVKRNDVRLLVDWVRVDGE"
     gene            complement(4971..5825)
                     /locus_tag="VFH83_15655"
     CDS             complement(4971..5825)
                     /locus_tag="VFH83_15655"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6487863.1"
                     /translation="MSQGKETTARRPSVGVRFRQRAVAYAVLAVIAVIYLGPVLTAVN
                     VALKTRQEFNLNPTSLATGLHVENFATAWKKASFPLYVKNSLLYAAVATALYVLTAVF
                     VAFPISRRYVKGSAFFFVLYVVALFLPNALIPQFQLMLHLGLYNTQAGYILLFLVNPI
                     GVVILVNYIRSLPREMDEAAAMDGCGYVRFVVSVAFPLMKPALASVIVLHAIGIWNEY
                     VTPLIYLTRKAYLPITRGLMVFYGQYGNDWTTLSAAILMLMIPMVALYLLLQRWIIAG
                     VLQGSVRG"
     gene            complement(5822..6760)
                     /locus_tag="VFH83_15660"
     CDS             complement(5822..6760)
                     /locus_tag="VFH83_15660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6487864.1"
                     /translation="MRRPLYHFGGKRSAFVPYLLMAPGLFLYLLVALGPSLATFVYSF
                     TDATGLVGTPTHWIGLANFREFLLLGDYSRDNLAILLRTLEFSLIVTVVQTAFGLLVA
                     LLLNRKLPGTNFFRALFFTPVILGVTIVGLIWTLFLYPLGGPAEKVLELFGTRSEFLG
                     GPPMQAFLWVIWVQIWANMGITMIIFLAGLQTVPGELLESARIDGAGGWRVFRAITFP
                     LLTPSLNTNLLLAIIGSLQAWQLLLVMKGVTNGTQVLGIWIYALAFGRQTANPTAVAM
                     RQGYGAAASVVLFLLVLVIGLSAQFLMQRRERRLLG"
     gene            complement(6875..8155)
                     /locus_tag="VFH83_15665"
     CDS             complement(6875..8155)
                     /locus_tag="VFH83_15665"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6487865.1"
                     /translation="MKRRWFVILAIALLVLPGALFGQQVTLELLIHQNQPLVDYINAF
                     NEKFQAKYPDVTVNVSVVKADELATSTQTRLTANDIDVVDMFGFAQGIQPFMKNVDKP
                     NWQTLIEAGMLLDLTGQPFLKNYDAAAIKDAGSFNGRVYEVNTGRYAFTGVFYNKDLF
                     AKYGVKVPNTWSQLLAAADTFKKNKIAPMTAGGKDGWPVLVAGYGILLSTYPDQAALV
                     KGLWTGSIKWNDAKSLEMWKRMQILSRDLIEPGASAISFDGAPGRFASGAAAMYPAGS
                     WDAPAIESANPSLNFGYFPMPGSDNPQDNKYLGGKYDVGWVVAGKTAKRDAALKWLDF
                     FSQPENYQAYVTAVGIMPTQPTAKLSTNLGKEVAPYLSSFRLGYELLWVGPKGAGQWA
                     QPNAAFFKPFNQWDDARKLADQVQSDLDAGLNAK"
     gene            complement(8152..8280)
                     /locus_tag="VFH83_15670"
     CDS             complement(8152..8280)
                     /locus_tag="VFH83_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487866.1"
                     /translation="MRSEVDRDVPGFERARAGALEPEGTGQRSKALEHDGSKEETV"
     gene            8465..10156
                     /locus_tag="VFH83_15675"
     CDS             8465..10156
                     /locus_tag="VFH83_15675"
                     /inference="COORDINATES: protein motif:HMM:NF027582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HET6487867.1"
                     /translation="MVLGRSAIDPSRAPAMLDTGTWDPAAAAAAISRGAESWAARAGG
                     APGSPGSPPASPVLSCEGKRVFLAELPDSQRVFLEVGPGGGGLLGKPFHQVRLGDGRF
                     LSAHPTDASVIDFFCRRLSPPNAPRALGRTPRIGIGTRMSTLVWPGAFAAMDEKGFAA
                     NTIQNSVRELNLLDDLRRGVPPERRYAAGFGMIESGYTGSTFEGLWVAGVLAALAHPR
                     PLAYGADADHIQLRRSDPSLEQAFRVVDAARYYTFFTIDAADILSYGALTDEASGDEH
                     ARRAIPDDRERREVLAWHEEAGRRGAPIPPDVLGRCVEKYWAAFRALGLIGERLAALR
                     SGAPYDLEFAFDEHPPEIAGPDCITTDEELRFVAREVTRRGLPVTHLAPNFGVEKGFD
                     YRIIDGLQGLEKRVASHQRIAGELGFLLDVHSADDLSAATCRAIGHATGGQLHYKISP
                     SLHLLFAQTVHDCAPEAFGPWWDDAVSYAREEARSGSRFAADCLAAWEAGARDRSPTQ
                     EVFRHFFFAYPGRRGPDGGFLHREALYSFPAGLYREYQSRVSSFLRAIAADLFGR"
     gene            10165..10728
                     /locus_tag="VFH83_15680"
     CDS             10165..10728
                     /locus_tag="VFH83_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487868.1"
                     /translation="MLFGQALREQGEERLVYSAPVGQRVAFAAIAAVIVASVAVVGTG
                     PLLSRLNLFSVALVAVCIFAAVYLERWTFDREANRFERNVGILPLYHRDSKPLDSLRK
                     VILRERGGRRGARPATGDGGPAEGPTPLGILTRGAATLCVVDQEGREYRLDIVRGSAA
                     SQLRKCAERLSAFCSIPMEEETEEGRA"
     gene            10725..10922
                     /locus_tag="VFH83_15685"
     CDS             10725..10922
                     /locus_tag="VFH83_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014769748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HET6487869.1"
                     /translation="MTDGRSRSAVKIGARVRIVLKQDQPTGRLTEGVVQDILTRSADH
                     PHGIKVRLTTGEVGRVKEILP"
     gene            complement(10919..11482)
                     /locus_tag="VFH83_15690"
     CDS             complement(10919..11482)
                     /locus_tag="VFH83_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487870.1"
                     /translation="MKKCVVVLLLLAVAVTAVQAQADKGPGFYVIPSAALAVGVNNYL
                     RGGEAVCQALYDIGFLSLGVEVKADYDLTFNVFNVPMLLVVGFGRNFWLAAGYTLPAG
                     SPSLTGTSGPIAWQLGGFPNTYEIGLNVLRFPTGIGTITIPTTISYTVNGPTNANDPL
                     FQALGMLVGALAGLKATVGVGLELKAF"
     gene            complement(11590..12918)
                     /locus_tag="VFH83_15695"
     CDS             complement(11590..12918)
                     /locus_tag="VFH83_15695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6487871.1"
                     /translation="MTAPSAPAPGNGLDSVARRHFLRNALLIGLENAGFVVAIAFVGT
                     NTVLPTFIVRLGGSTFLVGLAATCQTAGWLLPQLFGAGLLAGKSRIMPYLLRPLYIGR
                     PLILAVAGATVLLGARSPGVLMGILFAAILGFFVTDGISSVAWFELIAKAVPPDRRGR
                     LYGSAQIAGGVGGMAVGALVAVVLKSPGLPFPRNYALLFGLSGTLFLLNVLPFLFVRE
                     PVHERPAEEPTAPLRVRGFLLSLVRILKEDRTFVRLTGSRLLFGLATSAFPFYILFMD
                     RELSLSPDQLGLFVSSQVFGGLLGGLAIGWIADHAGPRTVIRLSGVVCGIVPCLALAM
                     SLLPHALGPALVPLGTLLFVLVGVVGSASFIGFMNYLMEVSPLEQRTSYVGLFNSLAG
                     ALMVAPPVLGWLVQAVSFRALFLVTIVASLGSLLVSLGLRRPPRRQGNAP"
     gene            complement(12915..13925)
                     /locus_tag="VFH83_15700"
     CDS             complement(12915..13925)
                     /locus_tag="VFH83_15700"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6487872.1"
                     /translation="MNARENMIRAVEFRRPERLPICFETLGMDDLRWVGWNQIGTGDH
                     EKRETLDEWGCLWVRSAARNMGQVKGHPLESWDAASSYRWPDPADPSFFQGMERKLEG
                     TDGYYRVTSIFMLLFERMHSLHGFQNTLMDLALERSRMEELADRIVEFDLGIIEGISR
                     RFPGAIDGFNFTDDWGTENALFIDPRLWDDFFKPRYKRIFDACHAAGWHVWMHSCGKV
                     NAIIPGLIEIGVNVLNLQQPRALGIEAVGKAFAGKVAFSSLCDIQKTLPTGSEAEIRE
                     EASLLLRSWGTEDGGFVLSDYGDGVAIGAADTQKRIMLDAFLGADRWRKTPAGGASRR
                     SP"
     gene            14074..15210
                     /locus_tag="VFH83_15705"
     CDS             14074..15210
                     /locus_tag="VFH83_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487873.1"
                     /translation="MTARERFVRTLLFQDVDRIPLEPGWGRESTLAAWHTQGLSAGVE
                     DAAAAAAEAYREAGGCLPLPQTGEPFPVDSRMIPRFEEKVLEQRGDTQIVQDWKGNVC
                     EISSRFGTRHLREAIDFVTRRWIRCPVESRADWPDLARRYDARDPSRLPPDASQVGAR
                     LKGRTWPLSLSINGPFWQLREWLGFERLCLLFYDDPAFLSDMVALWSDFVTRLLERVF
                     EHFVPDEVHLSEDMAFKEHAMIAPALVRESLLPVYRRWGALVREAGCPIYAMDSDGFV
                     GELVPIWIEAGINACDPIEVAAGNDVNGFRAAFGRRMAFRGGVDKRAIARGGDAIGRE
                     LERLAPVIHDGGFIPGCDHGVPPDVSWPDYVRFTGLLARATGWL"
     gene            15296..>15919
                     /locus_tag="VFH83_15710"
     CDS             15296..>15919
                     /locus_tag="VFH83_15710"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET6487874.1"
                     /translation="MDATPINWYRSPISRDDLRRFTEKSDLKGWLQAGSFLLIFLCTT
                     AGAVILFELRLWIPMVVVCYVHSAFLQMIGMSAAVHELSHGTVFRRKGPNEFFYHLFC
                     FLTWNSPVHFRNSHMLHHQLTVYRGRDKEVIQGPVKDKMNLGNYLSWITFDYQWFWTL
                     VSINIRHALGDGDADFFSWDPLFDRDDPRRRQMIRWARFAVLSYVVLI"
BASE COUNT         2755 a         5232 c         5305 g         2627 t
ORIGIN      
        1 agagaagtct ggcggactgg acggtgaggc agttggcggt attgggcagg gcccacccgg
       61 tgatgatgtg ggccagggat ccgccctcga agaggaccga ggtgtccacc gtgtcgtatc
      121 cctgtactgg gatcggtgaa agggaaggaa gcttctggcg ataaccgaca gcccggatct
      181 ccaggggccg cagacccgtg atgaacagga aggtgtccac catgtgagag gtgaggaacg
      241 agatggggct gttcttctcg gcgaagtcgg ctgaggagaa gaaccgcggc tcgtggttgg
      301 gatccgagac caggagacgg tcgagcatgt accagacgga ggactgaagc ggcccgtaga
      361 tgccggaccg taccctggcc cgcgcctcct tggtcacggg gtcctcccgc ttgtggaaat
      421 ccacccccag aaagcggcct gacgcgcgga cggcctccat catggcgtgc gcgtcgacca
      481 cccgatccgc cgtcggcttt ggcaggatca cgtccaggcc ggccgccaga gcgcacaggg
      541 tcggctcgcg atggagaccg tccggagtgt tgacggcgac agcgtccagc tccacgcgtc
      601 gaatcatctc ggtgtagctg gtgaacctcc ggttttccgg gatcttccag gcatcggcaa
      661 aagcggagag ggcctgggga ctggtgtcac agaccgcctc cacacggatg ccgccggagt
      721 actggctgcg gaagtacagg tactggtaga tctcccggac cacggaaccg gtgcccacca
      781 cgccaagcct tacggcggat gactgcatgg aagcctccct ggtcgatcgc ggatcccagc
      841 ggtgcgccgc acggcgcggg caccgggcat cacgcgcagc gctccatggc gcggagcgcg
      901 gcgcagttgg cgctcgggcc ttgcggcgtc ggtcgcccgg agcaataatg ccccaagcca
      961 tagccgaacg gaaggggtac gccatgaaga gggaccggat gagctcccgg gagaggtttc
     1021 acgccacgtt cgagtacgga actccggatc gcgcgttcct gtttcccgac tggtgcttca
     1081 acgacacgat cctgcgctgg aggcgggagg gactgcctcc gtcccagcac ttcaacgcct
     1141 tcttcggctt cgaccgcatg gccaccatcc cgctcaaccc ggggtacacg gaggacctgg
     1201 acgcggtctg gccccggccg gcgacacgcg tggtggaccg cagcctggag tggcacgtgg
     1261 tggagaacga gctgggcggc cggtatcggg cctggctgga ccgcgagatc ggcatgagcc
     1321 agtggatcga cttcccggtc cgcgaccggg agagctggga gcggttcaag aagtggctgg
     1381 atcccgacca gcccagccgg taccccgagt actgggacga cctggtgcgg tgctaccgcg
     1441 ggcgggacta cccgctgggc atcaccgccg gctcgttcta cgggtggctt cgggattggg
     1501 ttggcatgga gaacctggcc ctctggtact acgactgccc cgacttggtg cacgagatcg
     1561 tcgagtacac cgcggacttc gtcatccggt ggacggcacg ggcgctgacg gacgtgccgg
     1621 acatcgactt cgcctcgatc tgggaagaca tgtgtatgaa gaccgggccg ctcatctccc
     1681 ccgagctcta ccgtgcgttc cacgtcgagc ccatgaagcg tgtcatcagg gccttccgcg
     1741 acgctgggat caggatcatc accctggatt ccgacggcat ggtggattcc ctcatcccca
     1801 tctggatgga tgccggcatc gacgtgatct atcccatcga gcgcgcctcg gggtgcgacc
     1861 cggtccgcta ccggacccaa tacgggaaga agcttcgcat gtacggcggc atcgacaagc
     1921 gcgtgcttcg cgacggaatg ccgaagagcg cgatcgagaa ggaagtcgga gagaaggcgg
     1981 agctcattcg ccagggcggc tacacgcccc agatcgacca cgtgatcccg cccgatatcc
     2041 cgttcgaaaa cttccgctac tacgtggacc tggtccagga ggcgtgccgg ttcgggtaag
     2101 gcgcaggcgc cacgcgagga ccgcctccgg ggcgcgcccc gatcaatcct tctggtacca
     2161 gcgcacccag tccacgtcca tgctcgcgga ggagaacccg atcggcggga tctggttgga
     2221 caggaacctc cccccgacgg caaggttcag gagaatgaag aagggccggc ctcggaaggc
     2281 ttcccgatcc gcctcggtca ggctctgggt cacgtagggg atgccgtcca gataccacgt
     2341 cagcgtcgta ccgctccact cgacgccgaa cacgtgatac gcgtcgccga acgcgcccga
     2401 cgggagcctg gccacgcccg ggatcgaggc ggcatgccca cccgaggcat cccgccagtg
     2461 catggtcccg agcaccgtcc catcatcgcc gccccgcatc tccaggatgt cgatctcccc
     2521 acacgcgggc caccccgcgg aggtgatgct ctcccccatc atccagaagg caggccagag
     2581 tccccgaccc gagggcagtc ggatccgcgc ctccaccagg ccgaacgcca tggatcgctt
     2641 cccctgggtc ttgatacgcg ccgaggtgta gtacctgccg ccatatcgct cgcttcgggc
     2701 ggtgatccgc agcacccggg aagaaccatc cgggacgatg gagaggttct cgggtctgtc
     2761 ggtgtaagac tcgccttccg cgtttcccca ccccgggttc tgcggcgagc cgtcgcccag
     2821 ctcggcggtc cacgcggaag tgtccagggc agggccgtcg aactcgtcgc tccacgcgag
     2881 gtgccacccc gcgcggccct gggcggggtc ggaatcgcac caggcggagc cggcgctcag
     2941 cgccacgcaa cctgccaaag ccagggctac ggcgcgggcg ctcatggccg acgaagccac
     3001 gccgtgcgga ccgtcttccg cgcgccccgg gccggcacgt tcaggcgggg gctcttgcgg
     3061 cgcgtgaggc tgtccgcgac ctcgggcacc gagaagaagt gctccaggaa catgcccgcc
     3121 gccccgtacg ctacggccag gtcccccagg gccgcgaact caatggaaca gtccaccgtg
     3181 tccttgtagg accagttccc ctggatctcc tcgacgagaa tccggctgag ctcttcgcgg
     3241 taagcggcca tctctccccc aatgacgacg gacttcaggt tcagcgtgtt caccaggaag
     3301 gcgatgtggg acgcgagctc tcgaagcacg cccgagagga gccgggggtc cgacttgatc
     3361 cttcgggaat cgtcgtcgga caccgagaac tggccgtggt tggacggctt ccagaggatg
     3421 ctctggaact ctccggccga gaatccttct ccgcggtaga ccttgccgtt caggacgaac
     3481 cccaggccca ccgcgattcc ccagtattcc tccccggtgg tgcgcccctt tcggaactcg
     3541 cccagcacga agcagaagtt gcggtggcgc tcggtcttgc ggaaggcgag ctcgccccag
     3601 cagcagcagt tggcgtcatt ctcgatcagc accggaacgc ccacgcgctc cgccacggcc
     3661 gcgcggaacg gcagcgggct gtgaacgtcc agggggttgg acttggtgat gactccctcc
     3721 gccgcgtcca cgacgccggc cagacccacc ccaacgccga tcaggggcat acggatcttc
     3781 cgcgcccgcc gttggatgtc ctccaggaag gagagaaagg tggcggccgg gtcggcggcc
     3841 cggaactcga tgggctgaga gtgggagaag atgacctccc ccttgagatc gatggccacg
     3901 gccttgccga agtccgtctg catctccacg cccagcacgc aaccgtattc cccgttgatg
     3961 gcgaggtgga gagggctccg tcccccgctg ggtcccgagc ggccgcgccg cgccaccgtg
     4021 accaggttgc ggcgcatgag gtccgccacg atcttggtca ccgtcgcctt gcccagcccg
     4081 agctggcggg agatctcggc ccggctgatg ccgccgctca gccacagctt ctgaaggacg
     4141 agggatgtgt tgatctcgtt gaggttattc gccatccacg cgcacccaat ccaccagcag
     4201 tcgaacgtcg ttccgcttca cccgctgcac ggtggaggcc ggcagacccg gatacgggga
     4261 gcggatcccg ttggtcttga gcggatagtt cccgcccagg gcgacgttga ggatgaggta
     4321 cttcggatcg tcgaacaccc aacgcccata gtgctcgacc atgggtcgaa ggacccgata
     4381 gatcacctca tcgtccacgg agaaaacgat gctgtccgcg ccgcacgcta ccgagtagac
     4441 ccgccacgcg gtggcatccc ggccgctgaa gtaccgtcgg ttcaccagcg gggtctctcc
     4501 cgcgtacccc ggaccatgga gcgcggcgtt cacccactcc gggtccccga cgttctccat
     4561 gatgtcgatt tccccggcct ccggccagct cccgttgccc agggcccaga aggccggcca
     4621 gaccccctgc cccgccggca gcttgatgcg cgcgctgacc gttccgtgcc gcagggagag
     4681 cttgccccgg gtgtcgatcc gacccgagac gaaatcgaac cttcggtctt ccgcggtcag
     4741 atagccctgg gagtatcgag gctggaggat cagcgctccg tcacaatccg cgtccgcctc
     4801 ccgacccggc gcgcatcgga cggtggtggg ggagtcgatg taggcctgtt gctcgtcgtt
     4861 gacgacggcg cccgtcaccg cgacgttcca cagggtccga tcgagctccg ctgaagtgaa
     4921 gtggtcgcag accatcatcc ccccttgttt gctgcggtaa cggccggcgc ctatccgcga
     4981 acggatccct gcagcacccc ggcgatgatc caccgctgga gcagcaggta cagcgccacc
     5041 atggggatca tcagcatgag gatggccgcg gaaagcgtgg tccagtcgtt gccgtactgg
     5101 ccatagaaca ccatgaggcc ccgcgtgatc ggcagatagg ccttacgggt gagatagatc
     5161 aggggagtga cgtactcgtt ccagatcccg atggcgtgca ggacgatgac gctggccagc
     5221 gcgggcttca tcagcgggaa ggccaccgag accacgaacc gcacgtaccc gcacccgtcc
     5281 atggccgcgg cctcatccat ctcccgtggc agggaccgga tgtaattcac caggatcacc
     5341 acgccgatgg ggttcaccag gaagaggagg atatagccgg cttgcgtatt gtagagcccc
     5401 aggtggagca tgagctggaa ctggggaatg agcgcgttgg gaaggaacag ggctaccacg
     5461 tacaggacga agaagaaggc gctccccttc acgtaacggc gggagatggg gaaggccacg
     5521 aacaccgccg tcagcacgta gagcgccgta gccaccgcgg cgtacaaaag ggaattcttc
     5581 acgtacagtg ggaagctggc cttcttccac gccgtggcga agttctcgac gtgcagcccg
     5641 gtggccaggg acgtgggatt gaggttgaac tcctggcggg tcttcagcgc cacgttgacg
     5701 gcggtcagga ccgggccaag gtagatgacg gcgatcaccg ccagcacggc gtacgccacg
     5761 gcgcgctggc ggaagcggac gccgacggag gggcggcgcg cggtcgtctc cttcccctgg
     5821 ctcatcccag cagcctccgc tcacggcgct gcatgaggaa ctgcgcggag agcccgatca
     5881 ccaggaccag gaggaagagg accacggagg ccgcggctcc atatccctgc cgcatggcaa
     5941 cggcggtggg gttggccgtc tgacgaccga acgccagggc gtagatccag atgcccagga
     6001 cctgcgtgcc gttggtgacc cccttcatga ccaggaggag ctgccacgcc tggagggacc
     6061 cgatgatcgc gaggagcagg ttcgtgttca ggctgggcgt gagcagcggg aacgtgatgg
     6121 cccgaaagac acgccagccg cccgcgccgt cgatccgcgc cgactccagg agctcgccgg
     6181 gcacggtctg caggcccgcc aggaagatga tcatcgtgat ccccatgttg gcccagatct
     6241 gaacccagat gacccacagg aacgcttgca tcggcgggcc gcccaggaac tcgcttcggg
     6301 tgccaaagag ctcaagcacc ttctctgcgg gtcctcccag tggatagagg aagagggtcc
     6361 agatcaggcc aacgatggtg accccgagga tcactggcgt aaagaaaagc gcgcggaaga
     6421 agttggtccc ggggagcttg cggttgagga gcagcgccac cagcagccca aaagcggtct
     6481 gcaccaccgt gacgatcaga gagaactcca gcgtgcgcag gagaatggcc aggttgtccc
     6541 gggagtaatc acccaggagc aggaactctc ggaagttcgc cagaccgatc cagtgggtcg
     6601 gcgtgcccac caggccggtg gcgtccgtaa aggagtagac gaacgtggcc agggacggcc
     6661 ccagcgccac caggagatac aggaacagcc ccggcgccat caggaggtac gggacgaacg
     6721 cggacctctt gccgccgaag tgatacaacg gccgcctcaa gggatcacct ctcgccgagc
     6781 gtacccgaag ggcttaccgg ggcccagagg ccgttcagag cccaaaggct tcccggggcc
     6841 ctggggcccc gggaagcggt catccggcgc tttttcactt ggcgttgagt ccagcatcga
     6901 ggtcggactg aacctggtcg gccagcttcc tggcgtcgtc ccactgattg aacggcttga
     6961 agaatgccgc gttgggctgg gcccactgac cggcgccctt gggtccgacc cacaggagct
     7021 cgtagcccag gcggaagctg gagaggtacg gagccacttc cttgccgagg ttcgtggaga
     7081 gcttggcggt aggctgggtg ggcatgatgc cgaccgcggt cacatacgcc tggtagtttt
     7141 ccggctggga gaagaagtcc agccatttca gggcggcgtc cctcttggcg gtcttcccgg
     7201 cgaccaccca acccacgtcg tacttcccgc cgaggtactt gttgtcctgc gggttgtcgc
     7261 tgcccggcat ggggaagtag ccgaagttga ggctggggtt tgcgctctcg atggccggcg
     7321 cgtcccacga gcccgcggga tacatggccg ccgcgccgga ggcgaaccgg cccggcgctc
     7381 cgtcaaacga gatagccgat gcgcccggct cgatcaggtc cctcgagagg atctgcatcc
     7441 gcttccacat ctccagggac ttcgcgtcgt tccacttgat gctgccggtc cacagccctt
     7501 tcaccagggc ggcctggtcg ggatacgtgg acagcaggat gccgtagccg gccaccagca
     7561 cgggccatcc gtcctttccg cccgcggtca tcggagcgat cttgttcttc ttgaacgtgt
     7621 ccgccgcggc caggagctgg gaccaggtgt tgggcacctt caccccgtac ttcgcgaaga
     7681 ggtccttgtt gtagaagacg cccgtgaagg cgtagcggcc ggtgttcacc tcgtacaccc
     7741 tgccgttgaa cgagccggcg tccttgatcg ccgccgcatc gtagttcttg aggaagggct
     7801 gcccggtcag gtccaggagc atccccgcct cgatcagcgt ctgccagttc ggcttgtcca
     7861 cgttcttcat gaagggctgg atgccctggg caaacccgaa catgtcgacg acgtcgatgt
     7921 cgttggcggt gagccgggtc tgcgtggagg tggccagctc gtccgccttg accacggaca
     7981 cgttcaccgt gacgtcggga tacttggcct ggaacttctc gttgaaggcg ttgatgtagt
     8041 ccaccaacgg ctggttctgg tggatgagaa gctccagcgt gacctgctgt ccgaagagcg
     8101 cgcccggcag gaccaggagc gcaatcgcca ggatcacgaa ccaccgtctc ttcataccgt
     8161 ctcctccttc gagccgtcgt gctcgagcgc tttcgacctc tggcccgtgc cttccggctc
     8221 gagcgctccg gctcgagcgc gctcgaagcc gggaacgtca cgatcgactt cgctcctcat
     8281 ccaggctctc ttccgatgtt agttcggctc caatccgaac taaccgatcc taccacaggt
     8341 tccgggtttg tcaagaaaaa tcgcctctgc gttcggcgcg ccgcggaaga gccgatccgc
     8401 gggccgctcc gttccccgat gcgcgagggc ccccatcctg ctacactgac cggggaggtg
     8461 gcgcatggtt ctgggccggt cggctatcga tccttccagg gctccggcga tgctggatac
     8521 gggaacctgg gacccggccg ccgcggcagc ggcgatctcc cgcggcgcgg agtcctgggc
     8581 ggctcgcgcg ggaggcgcgc cggggtcccc cggctcccct cccgcttccc cggttctgtc
     8641 ttgcgaaggc aagcgggtgt tcctggcgga gctccccgac tctcagcggg tcttcctcga
     8701 ggtaggcccc ggaggcggag gcctcctcgg aaagccgttc catcaggttc gtctcggtga
     8761 cggcaggttc ctgagcgccc atccgacgga cgcctccgtc atcgacttct tctgccggag
     8821 gctctcgccg ccgaacgccc cgcgggcgct cggccggacc ccgcggatcg gcatcgggac
     8881 ccgcatgagc acgctggtgt ggcccggcgc gttcgcggcc atggacgaga aagggtttgc
     8941 cgccaacacc atccagaact cggtgcgcga gctgaatctc ctggatgacc tgcgccgggg
     9001 cgtgccgccg gagcgaaggt acgccgcggg gttcggcatg atcgaaagcg ggtacacggg
     9061 aagcacgttc gagggtctct gggtggccgg cgtgctcgcc gccctggcgc atcctcgacc
     9121 cctggcgtac ggcgcggacg cggaccacat ccagctccgg cgctccgacc cgagcctgga
     9181 acaggcattc cgcgtcgtcg acgccgcgcg gtactacacc ttcttcacga tcgatgcggc
     9241 ggacatcctc tcctatggcg cgctcaccga tgaggcttcc ggtgacgagc atgcgcggcg
     9301 ggccatcccg gacgatcgcg agcggcggga ggtgctggcg tggcacgaag aagcgggacg
     9361 ccgcggcgct ccgatccctc ctgacgtcct cggacgatgc gtggagaagt actgggcagc
     9421 gttccgagcg ctgggtctca tcggggagcg gcttgccgcc ctgcgctcgg gtgcgcccta
     9481 tgacctggag ttcgccttcg acgagcaccc gcccgagatc gccggtcccg actgcatcac
     9541 gacggacgaa gagctgcggt tcgtggcccg cgaggtgacc cggcggggtc ttcccgtaac
     9601 acacctggca ccgaacttcg gcgtggagaa gggcttcgac tatcgcatca tcgacggtct
     9661 gcagggtctg gaaaagcgcg tcgcctcgca ccagcgaatc gccggtgagc tcgggtttct
     9721 gctggacgtc cactcggcgg acgatctttc ggcggcaacg tgcagggcca tcggccacgc
     9781 caccggtggg cagctgcact acaagatctc gccgtcgctt cacttgctct tcgcgcagac
     9841 ggtccacgac tgcgcgccgg aggcctttgg tccctggtgg gacgatgccg tctcttacgc
     9901 gcgcgaggag gcgcgaagcg ggtcccggtt cgcggcggat tgcctggcgg catgggaggc
     9961 cggagcaagg gatcggtcgc cgacccagga ggtctttcgc cacttcttct ttgcgtaccc
    10021 gggccgccgc ggtcccgatg gcggcttcct ccaccgcgag gcgctctact cgtttccggc
    10081 cgggctgtat cgggaatacc agtcccgggt gtcttccttc ctccgcgcga tcgcggcgga
    10141 tctttttggc cgataggggc agcggtgctt ttcggtcagg cgctgcggga gcaaggggag
    10201 gagcggctgg tctactcggc gcccgtgggg cagcgggtgg cctttgcggc gatcgccgcg
    10261 gtcatcgtgg cgtccgtcgc cgtcgtcggg acggggcccc ttctttcgcg gctcaacctt
    10321 ttctccgttg cgctcgtggc ggtgtgcatc ttcgctgccg tctacctgga gcgttggacg
    10381 ttcgatcggg aggccaaccg gttcgaaagg aacgtgggca tcctcccgct ctaccaccgg
    10441 gacagcaagc ccctggattc gcttcggaaa gtgatccttc gagagcgggg cggtcggcga
    10501 ggcgcgcgcc ccgcaacggg ggacggagga ccggcggagg ggccgactcc gctgggcatc
    10561 ctgacccgtg gagctgccac gctttgcgtg gtggaccagg aaggccggga gtaccggctg
    10621 gacatcgtga ggggcagtgc ggcttcccag ttgcggaagt gcgcggagag actttccgcg
    10681 ttctgctcca ttccgatgga agaggagaca gaggagggcc gggcatgacg gatggaaggt
    10741 cgcgttccgc ggtgaagatc ggagcccggg tccggatcgt tctgaaacag gaccaaccga
    10801 ccggtcgtct caccgagggc gtggtccagg acatcctgac ccggtcggcg gaccatcccc
    10861 acgggatcaa ggtccgcctc accaccggag aggtgggccg ggtcaaggag attctcccct
    10921 aaaacgcctt cagctcgagc ccgaccccga ccgtggcctt gaggccggcc agcgcgccca
    10981 ccagcatgcc cagcgcctgg aagagcgggt cgttcgcatt ggtgggaccg ttgacggtat
    11041 acgagatcgt ggtcgggatg gtgatcgtcc cgatgccggt cggaaagcgc agaacgttca
    11101 gcccgatctc gtacgtgttc gggaacccgc cgagctgcca cgcaatggga ccgctggtgc
    11161 cggtcaggga cggcgagccc gcgggcagcg tgtatcccgc cgcgagccag aagttgcggc
    11221 cgaacccaac gaccaggagc atcggcacgt tgaagacgtt gaaggtgaga tcgtagtccg
    11281 ccttcacctc cacgccaagc gacaggaatc cgatatcgta gagagcctgg cagaccgcct
    11341 ccccgccgcg caggtagttg ttcacgccta cggcgagcgc cgcggaagga atcacgtaga
    11401 accccggacc tttgtccgcc tgggcctgca ccgcggttac cgcaaccgcg agcagcaaga
    11461 gcaccacgac acatttcttc atcacattcc tcctgatgcc caggagtgta tcccgacgca
    11521 atggcccgtc aggaactttt ccgtgaggtt gccgggggtg cggcgggagg ccgggccagt
    11581 cgccggacgc tagggggcgt taccctgccg gcgcggcggt cttcgcagcc ccagggagac
    11641 gagcaggctt ccgaggctgg cgacgatcgt caccagaaac agggctcgaa aggacactgc
    11701 ctgcaccagc catcccagca cgggcggggc caccatgagc gctccggcca ggctgttgaa
    11761 gagcccgacg taactggtgc gctgctccag gggcgagacc tccatgaggt agttcatgaa
    11821 tccgatgaag ctggctgacc ctacgacccc gacgagcacg aagagcaacg tccccagcgg
    11881 cacaagggcc ggccccaggg catgaggcaa aaggctcata gccagcgcca ggcagggcac
    11941 gatcccacag accacaccgc tcagacggat cacggtccga ggtccggcat gatccgcgat
    12001 ccacccgatg gccagcccgc ccaggagccc tccgaagacc tgggaggaga caaagagccc
    12061 gagctggtcg ggactgagcg acagctcgcg gtccatgaac aggatgtaga acgggaaggc
    12121 cgaggtggcc agcccgaaga ggaggcggga acccgtgagg cgaacgaacg tccgatcctc
    12181 tttcaggatg cggaccaggg agaggagaaa gccgcgcacg cgcaggggag ccgtcggctc
    12241 ttccgccggt ctctcatgca cgggctcgcg cacgaacagg aacgggagga cattgaggag
    12301 aaacaacgtc cctgagaggc cgaagagcag cgcgtagttc cgcgggaagg gcagtccggg
    12361 gcttttcagc actaccgcca ccagcgcgcc tacggccata cctcccactc cgccggcgat
    12421 ctgcgcgctt ccatagagcc ggccgcggcg gtccggtggg acggccttcg cgatgagctc
    12481 gaaccatgcc acgctggaga tcccgtccgt gacgaagaac ccaaggatcg cggcgaacag
    12541 gatccccatc agcacgccgg gggaccgggc gccgaggaga acggtggctc cggccaccgc
    12601 caggatcagc ggcctcccga tgtacaaggg tcggagcagg taaggcatga tccgcgactt
    12661 gccggccagc aggcccgcgc cgaagagctg aggcagaagc cagcccgcgg tctggcaggt
    12721 ggccgcgagc cccaccagga acgtcgagcc ccccaggcgc acgatgaacg tggggagcac
    12781 cgtgttggtc cccacgaaag cgatcgcgac gacaaacccc gcgttttcaa ggccgatcag
    12841 gagcgcgttt ctgaggaagt gtctccgtgc gaccgagtcc agcccgttgc caggcgccgg
    12901 cgcggaaggc gccgtcacgg tgatcgacgc gacgcgcccc ctgcgggcgt tttgcgccag
    12961 cggtccgcgc ccaggaacgc gtccagcatg atccgcttct gcgtgtccgc ggcgccgatg
    13021 gctacgccgt ccccgtaatc ggacagcacg aatccgccgt cctcggtgcc ccagcttcgc
    13081 agcaggaggc tcgcctcttc ccggatctcc gcttccgagc ccgtgggcag ggtcttctgg
    13141 atgtcgcaga gggaagagaa ggcgaccttg ccggcgaacg ccttcccgac cgcctcgatg
    13201 cccagcgctc gaggctgctg gagattgagc acgttcacgc cgatctcgat cagaccgggg
    13261 atgatggcgt ttaccttccc gcaggagtgc atccagacgt gccatccggc cgcgtggcag
    13321 gcgtcgaaga tcctcttgta gcgaggcttg aagaagtcgt cccagaggcg cgggtcgata
    13381 aagagcgcgt tctccgtccc ccagtcatcg gtgaagttga agccgtcgat ggcgccgggg
    13441 aaccgccggg agatgccctc gatgatgccg aggtcgaact ccacgatccg gtccgcaagc
    13501 tcctccatgc gtgagcgctc cagcgcgagg tccatcaggg tgttctggaa cccgtgcagg
    13561 ctgtgcatac gctcgaagag gagcatgaag atgctcgtca cgcggtagta cccgtccgtg
    13621 ccctccagct tcctctccat tccctggaag aaggacggat cggcgggatc cggccagcgg
    13681 tacgaggagg cggcgtccca ggactccagg gggtgaccct tgacctggcc catgttccga
    13741 gcggccgagc ggacccagag acatccccac tcgtccagcg tctcgcgctt ctcgtggtcc
    13801 cccgtcccga tctggttcca tcccacccat cgcaggtcgt ccatccccag ggtctcgaaa
    13861 cagatcggca gcctctcggg ccgacggaac tcgactgcgc gaatcatgtt ctctcgggcg
    13921 ttcatccggt ctcctaagcc gcgccgccgt gaaggtggcg acgccggggt gtggcgctat
    13981 gatgtcagtc ttcgcgaatc tcggcaacgc tcaggcgcct cctctcgcgc gttgcggccg
    14041 ggggacgatc ctgctatacc tgcgatgcat cctatgacgg cgcgagaacg gtttgtccgt
    14101 accctcctct tccaggacgt cgaccggata ccgctcgagc ccggttgggg ccgggaatcc
    14161 accctggccg cgtggcacac ccaaggactc tcggcgggtg tagaggatgc cgccgcggcc
    14221 gccgccgagg cctaccggga ggcgggaggg tgtctgccac tgccgcaaac gggtgagccc
    14281 tttcccgtgg actcacgaat gatccctcgg ttcgaggaga aggtcctcga gcagcgcggg
    14341 gacacgcaga tcgtccagga ctggaaagga aacgtctgtg agatcagctc ccggttcggg
    14401 acgcgccatc tgcgcgaggc catcgacttc gtcacgcgcc gatggatccg ctgtcccgtg
    14461 gagtcacgcg ccgattggcc ggaccttgcg cgtcggtacg acgcgaggga cccgtcccgg
    14521 ctgccgccgg acgcctctca ggtaggggcg cgcctgaagg gccgtacctg gcccctgtcc
    14581 ctgtccatca acgggccgtt ctggcagctc cgggagtggc tgggcttcga aaggctgtgc
    14641 ctgctcttct acgacgaccc ggcgttcctg tccgatatgg ttgcgctctg gtcggacttc
    14701 gtgacccggc tgctcgagag ggtcttcgag catttcgttc ccgacgaggt gcacctctcc
    14761 gaggacatgg cgttcaaaga gcacgccatg atcgctccgg cgctggtccg cgagagtctc
    14821 ctgcccgtct accgacgctg gggcgccctg gtccgggagg ccggctgccc catctatgcc
    14881 atggactccg atggttttgt cggggaactg gtgccgatct ggatcgaagc cgggataaac
    14941 gcctgtgatc cgatcgaggt cgcggccggc aacgatgtca acgggtttcg cgccgccttc
    15001 ggtcgccgga tggcgttccg cggtggggtc gacaagcggg ccatcgcacg cggtggggac
    15061 gcgatcgggc gggaactgga gcggctggcc ccggtgatcc atgacggtgg cttcatcccc
    15121 ggctgcgacc acggcgtccc gccggacgtc tcctggccgg actatgttcg tttcacgggc
    15181 ctgctggcca gggcaaccgg ctggttgtag catgcggtgt gtggcgcgag gggcacggat
    15241 atggtagagt cgggaatccc cgacgatcca gtcccttcgt gaaagcgagg tcctcatgga
    15301 tgccaccccc atcaactggt atcgctctcc catctcgcgc gatgatctac ggcggttcac
    15361 ggagaagagc gacctgaagg gctggctgca ggcgggaagc ttcctcctga tcttcctctg
    15421 caccaccgcc ggcgcggtca tcctcttcga gctgcggctc tggatcccca tggtcgtggt
    15481 gtgctacgtg cactccgcgt tcctgcagat gatcgggatg tccgccgcgg tgcacgagct
    15541 cagccacggg acggtctttc ggcgaaaggg tccgaacgag ttcttctacc accttttctg
    15601 cttcctcacg tggaacagcc cggtccattt ccgcaacagc catatgctcc accaccagct
    15661 cacggtctac cggggccggg acaaggaagt gatccagggc cccgtgaagg acaagatgaa
    15721 cctggggaac tacctgagct ggatcacctt cgactatcag tggttctgga ccctggtgag
    15781 catcaatatc cgccacgcgc tgggggacgg agacgcggac ttcttctcct gggacccgct
    15841 gttcgaccgc gatgaccctc ggcgcagaca gatgatccgg tgggcacggt tcgccgtgct
    15901 ctcctacgtc gtcctgatc
//
LOCUS       DASRZS010000646         4434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3426062, whole genome shotgun sequence.
ACCESSION   DASRZS010000646 DASRZS010000000
VERSION     DASRZS010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3426062"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFH83_15715"
     CDS             <1..381
                     /locus_tag="VFH83_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HET6487875.1"
                     /translation="LIPELQGRFPLRVELSALTKEDFVKILTQPKNALITQYTELLKT
                     EGVTLDFQPESIERIAEIATEVNSRSENIGARRLHTIMELLLEEVSFHAPEISGQTIT
                     ITRQYVEERVKNVVEDQDLTRYIL"
     gene            472..888
                     /gene="flgB"
                     /locus_tag="VFH83_15720"
     CDS             472..888
                     /gene="flgB"
                     /locus_tag="VFH83_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HET6487876.1"
                     /translation="MTFSGAFGKNLDILSREMHVLTLRRDVISNNIANADTPNFKRSD
                     VNFESQLKRALDSEHPPAFQQAITNERDIPFSRPVDYRDVQPRRVLDYLTESKNNGNN
                     VDIEQEGMDALNNQLMYTTIAQAIGSQIQGVNIVLR"
     gene            900..1358
                     /gene="flgC"
                     /locus_tag="VFH83_15725"
     CDS             900..1358
                     /gene="flgC"
                     /locus_tag="VFH83_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HET6487877.1"
                     /translation="MGMFSSINTAASGLTAERLRLDVIADNIANANTTRTTDGGPFRR
                     SRVIFRPRVPEPYWRSPFLPAYLQDPVGKGVRVASIEKDYASDPRLVYDPTHPDAIKS
                     GPRAGYVEYPNVNIVNEMVDMIEASRSYEANSAVINGTKAMFLKALEIGR"
     gene            1362..1694
                     /gene="fliE"
                     /locus_tag="VFH83_15730"
     CDS             1362..1694
                     /gene="fliE"
                     /locus_tag="VFH83_15730"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HET6487878.1"
                     /translation="MAILDAAQAMGDVVTLARTNPKHLVGAGEPAPTEQNPESSFGQL
                     LINALGNVNDSQLTATRLSEQMITSPDSVNVEDVTIALAQANLALSMTKAIVDRAIAA
                     YREIINIR"
     gene            1755..3449
                     /gene="fliF"
                     /locus_tag="VFH83_15735"
     CDS             1755..3449
                     /gene="fliF"
                     /locus_tag="VFH83_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HET6487879.1"
                     /translation="MNEWLNRIREQLRALWTRWNRTQKIILFSIVGASILALILLISL
                     SAAPSMVALITSPVTDEKAKLAIEQKLDDLGAKYQLRSDNIFYVADEPTARRLRMILV
                     QENLMPQVNPWSLFDTEPWTITDFERNVNLQRAVTVQLEQHIKALDDVDNASVVLVIP
                     PTELFQADQKPTTASIMITPKPGSDFTTNRKKIEGVQRLIKLAVAGLTDDNIVITDQT
                     GQVLNDFADVANVDRLDLAKRELRVKSDLQQQYKAEILDALRPVFGSDRVTSRIDITL
                     DMSKVNSTAEEYSPIAVVPATQFAPAQYAPSIARSTQNIEEHFQGSGFNPEGPAGTEG
                     QTPPAYKDLSNLVGKYDKTSSTTNNEINKKVTTEERQPYVIDRLAIGVAIDGTWQEKY
                     DSRGQAVLGPDGRIQREYIAVADADLAKAKSLIQGMSGFDRTRGDVVEVQTIQFDRTQ
                     QFEKEDGVLRHALQVRRTVYAILIGLAGLVVLVLAYRLIAKEVERRRRLREEELARQH
                     QAMREAALRTAEEQGVEVEMSVEDRARMEMQENAANMAREHPDDVAQLIRTWLVEE"
     gene            3453..>4434
                     /gene="fliG"
                     /locus_tag="VFH83_15740"
     CDS             3453..>4434
                     /gene="fliG"
                     /locus_tag="VFH83_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HET6487880.1"
                     /translation="MAKQAAPKPAAVEKEEKGALAGAPSGGGRREGKGHKRSLTGRQK
                     AAVFLVTLGSEIASEIFKHLREDEIEQLTFEIARLESIEPPERDQVLMEFHELMMAQE
                     YISTGGIDYARELLEKSLGTQKAVDIINRLTSSLQVRPFDFIRRTDPSHLLNFIQQEH
                     PQTIALILAYLEPQKASIILGALPHEVQSDVAKRIATMDRTSPEVLREVERVLEKKLS
                     TLSSEDYTAAGGVESIVEILNMVDRSTEKTIVESLEEDDPELAEEIKKRMFVFEDIVL
                     LDDRAIQKVMREVDTAELAKALKSVDSEVQDKIYRNMSKRASTLLKEDMDY"
BASE COUNT          951 a         1392 c         1441 g          650 t
ORIGIN      
        1 ctgatccccg agctgcaggg ccggttcccc ctgcgcgtgg agctctccgc gctgaccaaa
       61 gaggatttcg tgaagatcct gacccagccg aagaacgcgc tcatcacgca gtacacggag
      121 ctgctgaaga ccgaaggggt caccctggat ttccagccgg agtccatcga gcggatcgcg
      181 gagatcgcga cggaggtcaa ctcccggtcg gagaacatcg gcgcccgcag gctccacacc
      241 atcatggagc ttctcctgga ggaggtatcc ttccacgcac cggagatctc cggacagacc
      301 atcacgatca cccggcagta cgtggaggag cgcgtcaaga acgtcgtgga ggaccaggac
      361 ctgacccgct acattctctg accgcccgcg gaccgtccac cgcacgttat aggaagttgc
      421 ggttcagggc gcctggaaac cggccgaaca taaagggagg gggaaacggc agtgacgttc
      481 tctggagctt ttggcaaaaa cctcgacatc ctcagccggg agatgcacgt cctcaccttg
      541 agacgggacg tgatctccaa caacatcgcc aacgcggaca cgccgaactt caagcgaagc
      601 gacgtgaact tcgagagcca gctcaagcgg gccctggact ccgagcaccc gcccgcgttt
      661 cagcaggcga tcaccaacga gcgggacatc ccgttctccc gaccggtgga ttaccgcgac
      721 gtgcagccgc ggcgggtgct ggattacctt acggagagca agaacaacgg gaacaacgtg
      781 gacatcgagc aggaaggcat ggacgcgctg aacaaccagc tcatgtacac caccatcgcc
      841 caggccatcg gcagccagat ccagggcgtc aacatcgtgc tgaggtagag ggaagcgcca
      901 tggggatgtt cagcagcatc aacaccgcgg catccgggct cacggcggag cgtcttcgcc
      961 tggatgtcat cgccgacaac atcgccaatg ccaacaccac gcggaccacg gacggcggcc
     1021 cgttccgcag aagccgcgtg atcttccgac cccgggtacc cgagccgtac tggcgcagcc
     1081 cctttctccc cgcgtacctg caggatcccg tgggcaaggg cgttcgggtt gcttccattg
     1141 aaaaggacta cgccagcgac ccgaggctcg tctacgaccc cacgcacccg gatgccatca
     1201 agagcggccc gcgggcgggg tacgtggagt atcccaacgt caacatcgtg aacgagatgg
     1261 tggacatgat cgaggcctcg cgctcgtacg aggccaattc cgcggtgatc aacgggacga
     1321 aggccatgtt cctgaaggcc ctcgagatcg ggaggtaatg catggccatc ctggacgcgg
     1381 cacaggcgat gggggacgtg gtcaccctgg ctcggaccaa tcccaagcat ctggtcgggg
     1441 cgggtgaacc ggcaccgacg gagcagaacc cggagagctc tttcgggcag ctactcatca
     1501 acgcgctggg caacgtgaat gacagccagc tcacggccac aaggctgtcc gagcagatga
     1561 tcaccagccc ggactcggtg aacgtggagg acgtcaccat agcgctggcc caggccaacc
     1621 tggcgctttc catgaccaag gccatcgtgg atcgggccat cgcggcctat cgagagatca
     1681 tcaacatacg gtaagagacg ggctgcgcag cgcagccccc gggagaggac gaagggtcat
     1741 gatggggagg ggacatgaac gagtggttga atcggatccg tgagcagctg agggcgctct
     1801 ggactcggtg gaacaggacc cagaagatca tcctgttctc gatcgtcggg gcgagcatcc
     1861 tcgccctgat cctgttgatc agcctgagcg ccgcgcccag catggtggcg ctgatcacca
     1921 gtccggtcac ggatgagaag gccaagctgg ccatcgagca gaagctggat gaccttggcg
     1981 ccaagtacca gcttcgaagc gacaacatct tctacgttgc ggacgagccc acggcgcggc
     2041 gcctgcggat gatcctggtc caggagaacc tgatgcccca ggtgaacccg tggtcgctct
     2101 tcgacacgga gccctggacc atcacggact tcgagcgcaa cgtgaacctg cagcgggcgg
     2161 tcaccgtgca gctggagcag cacatcaagg cgctggacga cgtggacaac gccagtgtgg
     2221 tcctggtcat tccgcccacg gagctcttcc aggcggacca gaagcccacc acggcctcca
     2281 tcatgatcac gccgaagccg gggtcggatt tcacgaccaa ccgaaagaag atcgagggcg
     2341 ttcagcgcct gatcaagctg gccgtggccg gcctcacgga cgacaacatc gtgatcaccg
     2401 accagaccgg ccaggtcctc aacgacttcg cggacgtggc caacgtggac cggctggacc
     2461 tggccaagcg cgagcttcgg gtgaagtccg atctccagca gcagtacaag gcggagatcc
     2521 tggacgcgct gcggcccgtg ttcggatcgg accgtgtcac ctcccggatc gacatcaccc
     2581 tggacatgag caaggtcaac tccacggcgg aggagtattc ccccatcgcc gtggtgcccg
     2641 cgacccagtt cgcccccgcg cagtacgctc cgtcgatcgc ccgctcgacg cagaacatcg
     2701 aggagcactt ccagggctcc ggcttcaacc ccgagggccc ggcgggcacc gaaggccaga
     2761 cgccgccggc ctacaaggac ctgtcgaacc tggtgggcaa gtacgacaag acttcgagca
     2821 ccacgaacaa cgagatcaac aaaaaggtca ccacggagga gcgccagccg tacgtgatcg
     2881 accgtctggc catcggcgtg gccatcgacg gcacgtggca ggagaagtat gattcccggg
     2941 ggcaggccgt gctcggaccg gacggcagga tccagcggga atacattgcg gtggcggacg
     3001 ccgatctggc caaggccaaa tccctgatcc aggggatgtc cgggttcgac cggacccgcg
     3061 gtgacgtcgt ggaagtgcag accatccagt tcgaccgtac ccagcagttc gagaaggaag
     3121 acggcgtgct gcgccacgcc ctccaggttc ggcgcacggt ttacgctata ctcattgggt
     3181 tggccgggct cgtcgtgctg gtactggcct accggctgat cgccaaggaa gtggagcgga
     3241 ggcggcgcct gcgcgaagag gagcttgccc gccagcacca ggcaatgcga gaggcggctc
     3301 tgcgcacggc ggaagagcag ggggtggagg tggagatgtc ggtggaggac cgggcccgca
     3361 tggagatgca ggagaacgcg gccaacatgg cgcgggagca cccggacgac gtcgcccagc
     3421 tcatccggac ctggctcgtg gaggagtagc gtatggcaaa gcaggcagcg ccaaagccgg
     3481 cggcggtcga gaaagaggag aagggggcgc tcgccggcgc gccctcgggc gggggtcggc
     3541 gggaaggcaa gggacacaaa cgctccctga cgggccggca gaaggccgcg gtgtttttgg
     3601 tcaccctggg gtcggagatc gcgtcggaga tcttcaagca cctccgggaa gacgagatcg
     3661 agcagctcac cttcgagatc gcgcggctgg agagcatcga gcctcccgag cgcgaccagg
     3721 tgctcatgga gttccacgag ctgatgatgg cccaggagta catctccacg gggggcatcg
     3781 attacgcccg ggagctgctg gaaaagtccc tgggcaccca gaaggccgtg gacatcatca
     3841 accgcctcac cagctccctc caggtgagac ccttcgattt catacggcgg acggacccgt
     3901 cccaccttct gaacttcatc cagcaggagc acccccagac catcgccctg atcctggcct
     3961 acctggagcc ccagaaggcc tccatcatcc tgggcgcgct cccgcacgag gtgcagtccg
     4021 acgtggccaa gcggatcgct accatggacc ggacctcccc cgaggtcctg cgcgaggtgg
     4081 agcgggtgct ggagaagaag ctctcgaccc tctcgtcgga agactacacg gcggccggcg
     4141 gcgtggagag catcgtcgag atcctgaaca tggtggaccg gtccaccgag aagaccatcg
     4201 tggagtccct cgaggaagac gaccccgagc tggcggaaga gatcaagaag cggatgttcg
     4261 tgttcgagga catcgtgctc ctggacgacc gcgccatcca gaaggtgatg agggaagtgg
     4321 acacggcgga gctggcgaag gcgctgaaga gcgtggattc cgaggtgcag gacaagatct
     4381 accgcaacat gtccaagcgc gcctccacgc tgctcaagga agacatggat taca
//
LOCUS       DASRZS010000647         8923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1148756, whole genome shotgun sequence.
ACCESSION   DASRZS010000647 DASRZS010000000
VERSION     DASRZS010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8923
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148756"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1767
                     /locus_tag="VFH83_15745"
     CDS             <1..1767
                     /locus_tag="VFH83_15745"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6487881.1"
                     /translation="NRVVPSRNLHSVREMVEGFHPDRLTEPALTMKRPDGYWTLSYGE
                     LREHVRALGTALLSIGVRPGDRVGLISENRSEWVITYLAVTCIGAVIVPYDILLKVEE
                     LSNTLRASGARLVFTSAEYLEKVTRAVAGAGEGRLMLFDAEAIRTAESSPGSRWSAST
                     FTSLVQMGTTLRAGGDGGYAKASVSPDDLAALIFTSGTTGTPKGVMLSHRNLVENGDG
                     VQMTTPLGPGDNWIIVLPFHHTYPTSMGVFTPLLTYGRITSVPSLKTHVLVGIMKDTG
                     ATCVPAMPLLIERLYKGILSTVKERPPIVRVMFRALFAVSRFLFRALGLRVGKLLFRS
                     VARELGVEKLRFFVSGGGPIAREIIDGMEALGLVTYQGYGLTEASPVVSSCCPAHNRP
                     GSVGLPLANVEVRIDAPDANGNGEILARGPNIMMGYLDMPDKTREVLDEDGWLHTGDI
                     GAQDRDGYLYITGRLKNIIVTKGGKNIYPEEIENLLMASPLLSDVLVIGKLDEEGGEY
                     PHAIVYPDPEALKAAASQKGRALTAEEIRALVGAEIRRCTANAAAYKVPHGFEVSEEE
                     LPKTSTRKVKRYLFAGSHGAPR"
     gene            1824..3488
                     /locus_tag="VFH83_15750"
     CDS             1824..3488
                     /locus_tag="VFH83_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HET6487882.1"
                     /translation="MTASSQPLVSQAGLEVLREGGNAADAAVAMAAAINVTEPCSTGI
                     GGDCFALFYDASTRTVAALNGSGRAPAGLTIDLARSQGLERQGQFSNPHHAHTVTVPG
                     ACAGWCDLAARHGTMPLSRLLAPAIALAEQGFPVAPMTAEHWAVGAEGQLSTARGGRA
                     LTINGRGPRPGEIFRNQGLARTLRAVAEGGAAAFYRGPIARDLARAVREEGGVLTEAD
                     LAAHQSTWDQPISVPYRGVRVWECPPNGQGLVALMALSVLSGFDIGSLPTDSVDRWHL
                     LIEAVRLGFADGIRYIADPRMTGVPVSQLLSREHGEERRSHLDLARARTGPLPGLAGG
                     VEPRPMGGVEPRPTGGVEPRPPGSGTDTVYFCCADGAGNACSFINSNYMGFGTGIVPS
                     TANGDSWGFSLQNRGMNFSLDPDHPNALAPGKRPYHTIIPGMMTREDGSLLGPFGVMG
                     GFMQPQGHVQVAVALLDDRVDPQSALDRVRFFVDPTAAHSVVSVEEGLPQALVGGLTR
                     RGHTVSAGVSGHARAAFGRGQVILRDRDGVLWAGSDPRADGCAMGF"
     gene            3552..4079
                     /gene="msrA"
                     /locus_tag="VFH83_15755"
     CDS             3552..4079
                     /gene="msrA"
                     /locus_tag="VFH83_15755"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET6487883.1"
                     /translation="MSGTSTARQELATFGGGCFWCMEAVLQRTEGVLSVASGYAGGTK
                     RNPTYEEVCTGETGHAEVIQVAYDPERLSYTDLLDMFWQAHDPTTPDRQGADVGSQYR
                     SIILYHDEKQKAAAEQSLQKAQAKVRAPIVTEIRPLETFWKAEDYHQDYYNQNRNAGY
                     CRLVISPKLKKLHME"
     gene            complement(4136..5035)
                     /locus_tag="VFH83_15760"
     CDS             complement(4136..5035)
                     /locus_tag="VFH83_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6487884.1"
                     /translation="MADTVSPAPRPSASPDGSSAPSGLAIAAVAACAVLWSTGGLFIK
                     VIVWSPFAIAGIRSLIGGLVLLVYLRRPRFTWSFAQIAGAVCYAACMIGFVTANKLTT
                     AANAILLQYTAPLYAAILASIFLGERTSALDWATIAVVMGGMLLFFLDRLSVGNMVGN
                     VIAITSGVFFAGAIVSFRAQRQGSSLESILLSHGITLAVAIPFLFTSGLPTLGGAAGL
                     LALGLFQMGLSSILLSYGVRYVSALQSLLISVLEPLLNPLWVFLLLGEVPGPRALAGG
                     VVILVAVTARSVLSLRLGRRWRA"
     gene            complement(5028..5840)
                     /locus_tag="VFH83_15765"
     CDS             complement(5028..5840)
                     /locus_tag="VFH83_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HET6487885.1"
                     /translation="MKSPKQIALEKELRGKLTGPARKAANLLLDDPEVQQLQEYANVV
                     SIKRLGFNDHGPVHMRTVVLNAITMAQLMNRAGVSLSLEADDAGTFDDSQVALFLGGL
                     LHDIGMSVGRADHEHASVELALPIMDRVLREVYGENVAARVVVRSMAVECIVGHMATQ
                     RIHSLEAGLLQVADGCDMEKGRARIPMMIATESRMGDIHKYSASSIEEVRLEKGSRKP
                     IRITILMDATVGFFQVEEVLLHKIDSSPAKPYIELHAGVIGKKLKCYLGCDG"
     gene            complement(5844..6368)
                     /gene="bstA"
                     /locus_tag="VFH83_15770"
     CDS             complement(5844..6368)
                     /gene="bstA"
                     /locus_tag="VFH83_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018780520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HET6487886.1"
                     /translation="MENIQYPVGRFRSDPDITAEKRAALIRAITELPVKLRRAVGGLS
                     ASQLDTPYREGGWTPRQITHHLADSHLNSYCRFKLALTEGNPTIKPYDQDAWAATTDS
                     RAEVEPSLRLLEGLHERWGILLASLTPADFARTFQHPESGPWTLDKALQLYGWHSLHH
                     VAQIEGLRDRKGWR"
     gene            6457..7695
                     /locus_tag="VFH83_15775"
     CDS             6457..7695
                     /locus_tag="VFH83_15775"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HET6487887.1"
                     /translation="MIRVNEGYLRLSSSYLFSEIRKRTDAFQAANPEKEVIRLGIGDV
                     TLPLAPTVVSAFRSAVEEMARADTFRGYGPDLGYDFLRQPIAEVDYRSRGVDISPEEV
                     VISDGSKCDTANIQELFATSLRIALPDPVYPVYVDTNVMAGRTGPFRDGRYGGLIYLD
                     CTEDNGYIPDPPKSRVDLIYLCFPNNPTGASITRGQLETWVDYAQKSKALILYDAAYV
                     AYIRDESLPRSIFEVPGARECAIEFRSFSKTAGFTGTRCAFTVIPKSCMAYDEKDSPH
                     SLLELWGRRQSTKLNGVSYPVQRAAAAVYTPSGSRETREQADYYLGNAGLVRKRLSAL
                     GLSCTGGDNSPYVWVKTAHDSWKAFDLLLQKAAVVVTPGSGFGRCGEGYLRISGFNDR
                     AMVEKAMDRVGSVAGELAGA"
     gene            complement(7776..8462)
                     /gene="ribA"
                     /locus_tag="VFH83_15780"
     CDS             complement(7776..8462)
                     /gene="ribA"
                     /locus_tag="VFH83_15780"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HET6487888.1"
                     /translation="MTKEELLAKMKGTPDFVLEEYYCKGVGAYCTDNEMCVQMVAKAN
                     LPTRYGTFALYGFYDNRDAKEHTAIVRGDVAGKERVPVRVHSECHTGDVWGSLRCDCR
                     DQLEAAMRFIAARPFGAILYLKQEGRGIGLLNKIKAYQLQDLGLDTIEANQFLDKPVD
                     ARDYKVAARIMELLGIRSVSLLTNNPDKIQQLTRDGINVVERIPVVIPANRFDKGYLD
                     VKKNKMGHLM"
     gene            8653..>8923
                     /locus_tag="VFH83_15785"
     CDS             8653..>8923
                     /locus_tag="VFH83_15785"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET6487889.1"
                     /translation="MIPDKVRVILEANGLVASEFEAGSTATSPLAAQKLGVKVGQIAK
                     SLLFAGKNGRFFMVMAPGDRRLSSAKLKAATGVKSRMATAEEALAA"
BASE COUNT         1474 a         2983 c         2993 g         1473 t
ORIGIN      
        1 aaccgcgtcg tcccctcccg aaacctccac tcggtgcggg agatggtgga gggtttccac
       61 ccggatcgcc tgacggagcc ggccttgacc atgaagcggc cggatgggta ctggacgctc
      121 tcctacggtg agctgcggga gcacgtgcgg gcgctgggga ccgcgctcct ctccatcggg
      181 gtccgcccgg gagaccgggt cgggctcatc tccgagaacc gcagcgaatg ggtcatcacg
      241 tacctggccg tgacgtgcat cggcgcggtc atcgttccgt acgacatcct gctgaaggtc
      301 gaggagcttt cgaacacgct gcgggccagc ggcgcccgcc tggtcttcac ctcggccgag
      361 tacctggaaa aggtgaccag ggccgtcgcc ggagcggggg agggccggct gatgctcttc
      421 gacgcggagg cgatccgcac agccgaaagt tcccccgggt cccgatggtc ggcgtcaacg
      481 ttcaccagtc tcgtgcagat ggggactacg ctccgcgccg gaggggacgg cgggtacgcg
      541 aaagcctcgg tgtctcccga tgacctggcc gcgctcatct tcacctccgg caccaccggc
      601 acgcccaagg gcgtgatgct gtcccaccgc aacctggtgg agaacggcga cggcgtccag
      661 atgacgactc ccctgggacc cggggacaac tggatcatcg tgcttccgtt ccaccacacc
      721 tatccgacca gcatgggagt gttcacgccg ctgctcacgt atggccggat cacctcggtc
      781 ccctcgctga agacgcacgt gctcgtcggg atcatgaagg acaccggcgc cacctgcgtt
      841 ccggcgatgc cgctcctgat cgaacgcctg tacaaaggga tcctctccac ggtgaaggag
      901 cggccaccga tcgtgcgggt gatgttccgg gcgctcttcg ccgtgagccg gttcctgttc
      961 cgcgcgctgg ggttacgcgt cgggaagctc ctctttcgat ccgtggcgcg ggagctgggc
     1021 gtggagaagc tgcggttctt cgtctccggt ggtggtccca tcgccaggga gatcatcgat
     1081 gggatggagg cgctcggcct cgtcacctac cagggatacg ggctgacgga ggcgtctccc
     1141 gtggtgtcga gctgctgtcc ggcgcacaac cggcccggct ccgtcgggct gcccctggcc
     1201 aacgtggagg tccgcatcga cgcgccggat gccaacggca acggcgagat cctggcgcgg
     1261 gggcccaaca tcatgatggg ctacctcgac atgcccgaca agacgcggga ggtgctggat
     1321 gaggacggct ggctgcacac cggcgacatc ggcgcccagg accgcgacgg gtacctctac
     1381 atcaccggcc gcctcaagaa catcatcgtg accaagggcg gaaagaacat ctatcccgag
     1441 gaaatcgaga acctgctgat ggccagccct ctgctctccg acgtcctggt catcggcaag
     1501 ctggacgagg aaggcggcga atacccgcac gccatcgtct acccggatcc tgaggcgctg
     1561 aaggcagcgg catcgcagaa gggacgcgcc ctgacggcgg aggagatccg cgccttggtg
     1621 ggggccgaga tccgccggtg cacggcgaac gcagcggcgt acaaggttcc ccacggcttc
     1681 gaggtctcgg aggaggagct gcccaagacc tccacgcgga aggtgaaacg ttacctgttc
     1741 gccggatccc acggggcccc tcgatgattg tgccggaccc tgttttccgg tcccgccgtt
     1801 cctcgatcgt ggcgcgaggc ggcatgacgg catccagcca accgctggtc tcccaggccg
     1861 ggttggaggt cctgcgggaa ggagggaacg ccgccgatgc ggcggttgcc atggcggcgg
     1921 ccatcaacgt caccgagccg tgctccaccg gcatcggggg agactgcttc gcgctgttct
     1981 acgacgcgtc gacgcgcacg gtcgcggcgt tgaacggctc cggacgagcc cccgcgggcc
     2041 tgaccatcga cctggcgcga tcccaaggtc tggagcgcca gggccagttc tcgaacccgc
     2101 atcacgcgca cacggtgacc gttcccggcg cctgcgccgg ctggtgcgac ctggcggctc
     2161 ggcacggaac catgccgctg tctcgtctcc tggcgccggc cattgccctg gcggagcagg
     2221 ggttcccggt agcaccgatg accgccgaac actgggccgt cggagcggag ggccagctct
     2281 ccaccgcgcg cggtggccga gcgcttacga tcaacggccg gggcccgagg ccgggggaga
     2341 tcttccgcaa ccaggggctc gctcggacgc tgcgcgcggt ggcggagggt ggagcggccg
     2401 ctttctaccg gggccccatc gcgcgagacc tggcgcgcgc cgtccgggaa gagggcggcg
     2461 tccttacgga ggcggacctg gccgcccatc agtccacctg ggaccagccg atcagcgtcc
     2521 cctaccgcgg cgtgcgggtc tgggagtgtc caccgaacgg tcagggactg gtggcgctca
     2581 tggcgctctc ggtcctgtcg ggattcgaca tcggttcctt gccgacggac tcggtggacc
     2641 gctggcatct cctgatcgag gcggtccggt tggggttcgc cgacgggatc cgctacatcg
     2701 ccgatccccg gatgaccggg gttcccgtca gccagctact ctcccgagag cacggggagg
     2761 agcggcgctc ccacctggac ctggcacgtg cacgaacggg tcccctgcct ggactggctg
     2821 gcggcgtaga gccccgccct atgggcggcg tagagccccg ccccacgggc ggcgtagagc
     2881 cccgcccgcc gggatccggg accgacacgg tctatttctg ctgcgcggac ggggccggca
     2941 acgcctgttc cttcatcaac tccaactaca tgggcttcgg gacgggcatc gtgccgagca
     3001 ccgccaacgg cgactcctgg gggttcagcc tgcagaaccg agggatgaac ttctccctgg
     3061 acccggacca tcccaacgcg ctcgcgccgg gcaagcgccc ctaccatacg atcatccctg
     3121 gaatgatgac ccgggaggac ggatctctcc tgggaccctt cggcgtgatg ggcggcttca
     3181 tgcagcccca gggtcacgtc caggtggcgg tcgcactgct cgatgaccgg gtggatccgc
     3241 agtcggcgct ggaccgcgtg cgattcttcg tggatccgac ggccgcacat tccgtagtca
     3301 gcgtggaaga ggggctgccc caggccctgg tcggagggtt gacccgccga gggcatacgg
     3361 tgagcgccgg ggtcagtggt cacgctcgcg cggcgttcgg ccgcggccag gtgatcctgc
     3421 gcgaccgcga tggggtgctg tgggccggca gcgatccgcg ggcggacggc tgcgcgatgg
     3481 ggttctgagc cgagatcccg tcattggccg cttctccgtc ggggctgtag attctggtct
     3541 cggaggtgtc gatgagcgga acgtcaacgg cccgacagga gctggctacc ttcggcggcg
     3601 gctgtttctg gtgcatggag gctgtcctgc agcggacgga gggggtgctc tccgtggcct
     3661 cgggatacgc gggcgggacc aagcgaaacc ccacgtatga ggaagtctgc acgggagaga
     3721 ccgggcatgc ggaagtgatc caggtggcct acgatcccga gcggttgagt tacaccgacc
     3781 tcctggacat gttctggcag gcgcacgatc ctacgacgcc ggatcgccag ggcgccgacg
     3841 ttggctccca gtatcgatcg atcatcctct accatgacga gaagcagaag gccgctgccg
     3901 agcagtccct gcagaaggcc caggccaagg ttcgagctcc catcgtgacg gagatcaggc
     3961 ccctggagac gttctggaag gcggaggact accaccagga ctactacaac cagaaccgca
     4021 acgccggata ctgccggctg gtgatctcgc cgaagctcaa gaagctccac atggagtagg
     4081 ccggcctcag cccgagccgg tcgatgtccg cctcaacggc tgtccgtgcg gggcgtcagg
     4141 ccctccacct ccggcccaac cgcaggctca gcacggagcg cgcggttacc gccacgagga
     4201 tcaccacgcc gcccgccagg gcgcgtggac cgggcacctc ccccagcagc aggaacaccc
     4261 acaaaggatt gagcagcggc tccaggacgg agatgagcag ggactgcagc gcgctcacgt
     4321 agcgcacccc gtaggacaga aggatcgagg acagccccat ctggaagagc ccgagggcca
     4381 gaagacccgc ggccccaccc agggtcggta ggccgctggt gaacaggaac gggatggcaa
     4441 ccgccagggt gatcccgtgg ctcagcagga tggactccag cgatgacccc tgcctctggg
     4501 ctcggaagct cacgatggct cccgcgaaga acacgccgct ggtgatggcg atgacgttgc
     4561 cgaccatgtt ccccacggaa agccggtcca ggaagaagag cagcatgccg cccatgacca
     4621 ccgcgatggt ggcccagtcc agcgccgagg tccgctcccc caggaagatc gacgccagga
     4681 tggcggcgta cagcggcgcc gtatactgca gcaggatggc gttggcggcc gtagtgagct
     4741 tgttggccgt gacgaagccg atcatgcacg ccgcgtagca caccgcgccg gcgatctgcg
     4801 caaaggacca ggtgaaccgt gggcgcctga gatacaccag aagcacaagg ccgccgatca
     4861 ggcttcgaat cccggcgatc gcaaaggggc tccagacgat gaccttgatg aacaggccgc
     4921 cggtgctcca caggacggcg caggcggcca cggccgcgat ggcgagcccc gacggggcgc
     4981 tggagccgtc cggcgacgcg gacggacgcg gggcggggga aacggtatca gccatcacag
     5041 cccaggtagc acttcagctt cttgccgatg actccagcgt gcagctcgat atacggcttg
     5101 gccgggctgg aatcgatctt gtgcagaagg acctcctcca cctggaagaa ccccacggtg
     5161 gcgtccatca ggatcgtgat gcggatgggt ttgcgcgaac ccttctcgag gcgcacctcc
     5221 tcgatcgacg acgcggagta cttgtggatg tcccccatcc tggactccgt cgcgatcatc
     5281 atcgggatgc gcgcccgtcc cttttccatg tcgcagccgt ccgccacctg cagcagcccc
     5341 gcctccaggg agtggatccg ctgcgtggcc atgtggccga cgatgcactc cacggccatg
     5401 gaacgcacca ccacccgcgc ggcaacgttc tctccgtaga cctcccgaag gacccgatcc
     5461 atgatgggca gcgcgagctc cacgctggcg tgctcgtggt ccgctctgcc taccgacatg
     5521 ccgatgtcgt gcagcaaccc tcccaggaag agcgccacct ggctgtcgtc gaaggtgcct
     5581 gcatcgtcgg cctccaggct caacgagacc ccggcgcggt tcatgagctg ggccatcgtg
     5641 atggcgttca ggacgacggt ccgcatgtgg accggtccgt ggtcgttgaa ccccagccgc
     5701 ttgatggaaa cgacgttggc gtactcctgg agctgctgga cctcgggatc gtccaggagg
     5761 agattggcgg cctttctcgc cggcccggtg agctttccgc ggagctcctt ctccagcgcg
     5821 atctgctttg gagacttcat cccctatcgc cagcctttgc ggtcccgcag tccctcgatc
     5881 tgcgcgacgt ggtgaaggct gtgccagccg tagagctgca gggctttgtc gagggtccac
     5941 ggtcccgact ccggatgctg aaaggtcctg gcgaagtccg cgggggtcag ggacgccagc
     6001 aggatccccc accgctcgtg cagaccctcc aggagccgaa gggaaggctc cacctccgcg
     6061 cgggagtccg tcgtggctgc ccacgcgtcc tggtcgtagg gcttgatcgt cgggttgccc
     6121 tcggtcaggg ccagcttgaa cctgcaatac gagttcaggt ggctgtcggc gaggtgatgg
     6181 gtgatctgcc gaggcgtcca gccgccttcg cggtacggcg tgtcgagctg cgatgcgctc
     6241 aatccaccca ccgcccggcg aagcttcacc ggaagctcgg tgatggctcg gatcagcgcc
     6301 gcgcgcttct ccgcggtgat atcgggatcc gagcggaagc ggccgaccgg gtactggatg
     6361 ttttccatgg acccgatagt agccggaatc ccgccagcat cacaacccgg ggcgctcgcg
     6421 aattgacgct gcggcgaggc gcggatataa ggatgcatga tccgcgtcaa cgagggctac
     6481 ctgaggctct cttcgtccta cctcttcagc gagatccgca agcgaacgga tgccttccag
     6541 gccgccaacc ctgagaagga ggtgatccgg ctcggcatcg gtgacgtcac cctccctctg
     6601 gcgcccacgg tcgtgtcagc tttccgatcc gccgtggagg agatggcacg cgccgatacc
     6661 ttccgcggct acggaccgga tctggggtat gacttcctgc ggcagcccat cgcggaggtg
     6721 gactaccgct cccgcggcgt ggacatcagc ccggaggagg tggtgatcag cgacggctcc
     6781 aagtgcgaca ccgccaacat ccaggagctc ttcgccacgt cccttcgcat cgccctgcca
     6841 gaccccgtct atccggtcta cgtcgatacc aacgtcatgg ccgggcggac cggacccttt
     6901 cgggacggcc gctacggcgg tctcatctac ctggactgca ccgaggacaa cggctacata
     6961 ccggatccgc ccaagtcccg ggtggacctc atctacctct gcttccccaa caaccccacc
     7021 ggcgccagca tcacgcgcgg acagctcgag acctgggtcg attacgccca gaagagcaag
     7081 gcgctgatcc tctacgatgc cgcgtatgtc gcgtatatcc gggacgagtc cctgccgcgg
     7141 tcgatcttcg aggtgccggg cgcccgggag tgcgccatcg agttccgcag cttctccaag
     7201 accgcggggt tcacggggac ccggtgcgcg ttcacggtca tcccgaagtc gtgcatggcc
     7261 tacgacgaga aggattctcc gcactccctg ctcgagctgt ggggccggag gcagtccacg
     7321 aagctcaacg gcgtctccta cccggtgcag cgagccgcgg ctgccgtgta cactccgtcc
     7381 ggctcccggg agacgcgcga gcaggcggat tactacctgg ggaacgccgg actggtgcgg
     7441 aagcggcttt cggccctggg gctttcctgc acgggcggcg acaactcgcc ctacgtgtgg
     7501 gtcaagacgg cccacgattc ctggaaggcg ttcgacctgc tgttgcagaa ggcggccgtg
     7561 gtcgtgacgc cgggcagcgg cttcggccgg tgcggtgagg gttacctgcg gatcagcggg
     7621 ttcaacgatc gggcgatggt agagaaggcc atggatcgcg tcggctccgt ggccggggag
     7681 ctggcgggag cctgaggtgc ccgggggctc catccgcgcg gcggccgcgc cgcaacgatg
     7741 cccgggccgt ctcgctgcca cgccgtggag cgcctctaca tgaggtgccc catcttgttc
     7801 ttcttgacgt ccagatagcc cttgtcgaag cggttggcgg ggatcaccac ggggatccgc
     7861 tccaccacgt tgatcccgtc ccgcgtgagc tgctggatct tgtccggatt gttggtcagg
     7921 agcgacacgg accggatccc caggagctcc atgatgcggg ccgccacctt gtagtcccgc
     7981 gcgtccacgg gcttgtccag gaactggttt gcctcgatgg tgtccagacc caggtcctga
     8041 agctggtagg ccttgatctt gttgaggagc ccgatccccc ggccctcctg cttcaggtac
     8101 aggatagcgc cgaacggtcg tgcggcgatg aaccgcatgg ccgcttcgag ctgatcgcgg
     8161 cagtcgcaac gcagggatcc ccacacgtcc cccgtgtggc actccgagtg cacgcggacc
     8221 ggtaccctct ccttcccggc cacgtcgccc cggacgatgg cggtgtgctc cttggcgtcc
     8281 cgattgtcgt agaaaccgta gagggcgaag gttccatacc gggtgggcag gttcgccttt
     8341 gccaccatct gcacgcacat ctcgttgtcc gtgcagtagg ctccgactcc cttgcagtag
     8401 tactcctcca gcacgaagtc cggagtgccc ttcatcttcg ctagcagctc ttctttggtc
     8461 acagtcttcc tgcacggtgt ctcagccact ggcatgcgat atccttccga ttcgcctgaa
     8521 tcccggtcaa aggtagtgcc gccgggatgc caggtcaagt ttctcggccg ccggcgcgcg
     8581 acgaaaccgt tccgcgggcg gtcggcggtc cgccgttgac cacggccggc gcgccgcgta
     8641 gactccgatg ccatgattcc ggacaaggtg cgcgtcatcc ttgaggccaa cggcctggtt
     8701 gcgtccgagt tcgaggccgg aagcaccgcc acatccccgc tcgcggctca gaagctcggg
     8761 gtcaaggtgg ggcagatcgc caagtccctc ctcttcgccg gcaagaacgg gcgcttcttc
     8821 atggtcatgg cccccgggga ccggcggctg tcttccgcca agctcaaggc cgccacgggc
     8881 gtgaagtccc ggatggccac cgcggaggaa gcgttggcgg cga
//
LOCUS       DASRZS010000648         4587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3832035, whole genome shotgun sequence.
ACCESSION   DASRZS010000648 DASRZS010000000
VERSION     DASRZS010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3832035"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFH83_15790"
     CDS             <1..369
                     /locus_tag="VFH83_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6487890.1"
                     /translation="RKQARELSGGQQQRVGIARALMQQPRMILADEPVASLDPVLAHS
                     ILGYLEQLNRVDHITVLCSLHYLDLVQRYATRVIGLRAGRLVWRGTGEDIRRMSDAEF
                     KEVYGEDAVRTSAGRAEGSR"
     gene            366..1877
                     /gene="phnE"
                     /locus_tag="VFH83_15795"
     CDS             366..1877
                     /gene="phnE"
                     /locus_tag="VFH83_15795"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HET6487891.1"
                     /translation="MKPNRASERPSLVSPPVAAACSFVLPGLGQALARSVWRGLLILG
                     SLGCIVGMLVWRITLIGHLQKTPSAMVAKTLQLQPGFLLLLLACILAIWLWNAWDAFL
                     QARDRRGGFILFALILFTFFALGWQISEIAPARLVTDFPQAMQPLGKVVWPWQAAVTR
                     DPVLLLAGASIQVPKSDAPGPAADAVESGQPHLVSVPSTGDLSKLDQDNQIVPGTTLS
                     LTGTGFKPGIPVQIWWTDPLGNSFQIRKSGAYLSVMPDPRGSFSTDIVMPYSIVPAEK
                     GKDQPHRVVARQPIGLSAPRVSEPLQIVLQRMVETIFMGMMATLFGIILAIPVSFLAA
                     RNIMSGSPVTVAIYYLTRTILNVIRAIEPLIWALIAVVWVGLGPFAGIVALTVHSVAA
                     LGKLYSEAIEGIDAGPIEAIQATGATRLQTIAYAVVPQMISPFVSFSIYRWDVNVRMS
                     TVIGLVGGGGVGFILVQYIHLLDYRAAGIAVWFIALTVAVLDYVSAQIRQRFV"
     gene            complement(1862..2581)
                     /locus_tag="VFH83_15800"
     CDS             complement(1862..2581)
                     /locus_tag="VFH83_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487892.1"
                     /translation="MDRFEVLLLVARPAAGKSEVIDYLRRLPPDMRLARFHVGELRVI
                     DDFPMIWGWFEEDAILERMGLPRLHTDAEGYFLRRELWDLLIHRLCLEYGKLLDDEPD
                     LRERGTAVIEFSRGTEHGGYRAAFKAMDPSVLRRAAALWIDVSYEESLRKNRQRFNPR
                     RAHSILEHGLPDDKLERLYRHIDWQDLTAADPSCLVVGDQRVPYAVMPNQDDVTTGRG
                     EALGARLEETLTRLWRLYTNR"
     gene            2729..3232
                     /locus_tag="VFH83_15805"
     CDS             2729..3232
                     /locus_tag="VFH83_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487893.1"
                     /translation="MRKAAMVVLVSLLALAPLGAFADSSGTVGLGLSMGLALPFGSTA
                     GIPSTDGLPSFNWGFYVDIPLISTFHITPSSELYRFQSTNATDVDLAFKFIVPISTFD
                     VYAGLEPGLTAVGQVLDAHVGLVGGATLRLVSNLDAFAQAKYTVVFDGNENVRVLHIN
                     AGILFAF"
     gene            3358..3687
                     /locus_tag="VFH83_15810"
     CDS             3358..3687
                     /locus_tag="VFH83_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6487894.1"
                     /translation="MARLAAEGELSARCAETLKALAHAHRLRIVAILCEGDETVIGLS
                     RRLSLKQAIVSQQLRILRMSGLVDACRVAGFARYRITQPRLRQLVTCLEGCHAVDVKR
                     RTGGGAR"
     gene            3684..4226
                     /locus_tag="VFH83_15815"
     CDS             3684..4226
                     /locus_tag="VFH83_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HET6487895.1"
                     /translation="MNVNVEAELATLKEKLEAMPGGENKLTMIVFSGGLDKQLAAMII
                     ATGAAAMGMKVILFFTFWGTAALRDPAKRVGGKSFMGKMFGMMLPKGRDKLKLSQMNM
                     AGMGTSMLKGLMQKKHVASLGELYEVAGQLAVKIYACEMSMDLMGFKREELIDYPELE
                     VCGVATMLAHAKDSAIHFFI"
     gene            4240..4479
                     /locus_tag="VFH83_15820"
     CDS             4240..4479
                     /locus_tag="VFH83_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HET6487896.1"
                     /translation="MAEHDVTKTLDYKGLKCPMPVVNISKEIGKVAVGQVIEVDTTDP
                     GSIADFPAWAKTMGHQVLEIAQEPGVIRIFVKRLK"
BASE COUNT          738 a         1510 c         1531 g          808 t
ORIGIN      
        1 cgcaagcagg cccgggagct ctccggcggg cagcagcaga gagtcgggat cgcacgagcc
       61 ctcatgcagc agccgcgcat gatcctggcg gacgagccgg tcgccagcct ggacccggtg
      121 ctggcccact ccatcctggg atacctggag cagctcaatc gggtcgacca tatcacggtc
      181 ctctgcagcc tgcactacct ggacctggtc cagcgctacg ccacgcgcgt gatcgggctg
      241 cgcgccggac ggctggtgtg gcgcggcacc ggggaggaca tccgacgcat gagtgacgcc
      301 gagttcaagg aggtctacgg agaggacgcg gtccgcacca gcgccggacg cgcggagggc
      361 tcgcgatgaa gcccaaccgc gcctccgagc ggccgtccct cgtttccccg cccgtcgctg
      421 ccgcctgctc cttcgtgctc ccggggctgg gccaggcact ggcgcgttcc gtatggcgcg
      481 gactgctcat cctcggctcc ctgggctgca tcgtgggcat gctggtctgg cggatcaccc
      541 tgatcgggca cctgcagaag acgccatccg caatggtcgc caagacgctg cagctccagc
      601 cgggcttcct cttgctcctg ctggcctgca tccttgcaat ctggctctgg aacgcgtggg
      661 acgcgttcct ccaggcccgg gaccgccgcg gggggttcat cctcttcgcg ctcatcctct
      721 tcacgttctt cgccctgggg tggcagatca gcgagatcgc gccggcgcgc ctggtcaccg
      781 atttccccca ggccatgcag ccgctgggga aagtggtctg gccctggcag gcggccgtta
      841 cccgggatcc ggtgctgctt ctggccggag cctcgatcca ggtgcccaag tccgacgcgc
      901 cgggaccggc cgcggatgcg gtggaatccg ggcaaccgca cctggtttcg gtgccgagca
      961 ccggggacct gtcgaagctg gaccaggaca accagatcgt gccgggaacc accctctccc
     1021 tgacggggac cggattcaag ccgggcatcc cggtccagat ctggtggacc gacccgcttg
     1081 gcaacagttt ccagatccga aagagcggcg cgtacctgtc ggtgatgccg gatccgcggg
     1141 gctccttttc caccgacatc gtcatgccct actccatcgt cccggccgag aagggcaagg
     1201 accagccgca tcgcgtagtg gcacgacagc cgatcggcct gtcggcgccc cgggtcagcg
     1261 agccattgca gatcgtgctc cagcggatgg tggagaccat cttcatgggc atgatggcga
     1321 cgctgttcgg gatcattctc gcaatccccg tgagctttct tgcggcccga aacatcatgt
     1381 ccggctctcc ggtcacggtg gccatctact acctgacgcg cacgatcctt aacgtcatcc
     1441 gcgccatcga gcccctgatc tgggcgctga tcgccgttgt ttgggtgggg ctcgggccgt
     1501 tcgccggaat cgtggcgctc acggtccatt ccgttgcggc gctgggcaag ctgtactccg
     1561 aggccatcga agggatcgat gcgggcccca tagaggccat ccaggcgacc ggagccacgc
     1621 gcctgcagac catcgcttac gcggtggtgc cccagatgat ctccccgttc gtctccttct
     1681 cgatctaccg atgggacgtc aacgtccgca tgtccacggt gatcgggctg gtgggcggcg
     1741 gtggcgtggg gttcatcctg gtgcagtaca tccatctcct ggattaccgc gccgcgggta
     1801 tcgccgtctg gttcattgcc ctcacggtag ccgtgctgga ctacgtgagc gcgcagatcc
     1861 gtcagcggtt cgtatagagg cgccagaggc gggtcagagt ctcctccagg cgcgccccca
     1921 gggcttcccc cctgccggtg gtcacgtcgt cctggttggg catgacggca tagggtaccc
     1981 gttggtcgcc gaccacaaga caggacggat cggcggcggt gaggtcctgc caatcgatgt
     2041 ggcggtacag ccgctccagc ttgtcgtccg gcaggccgtg ctccaggatg ctgtgggctc
     2101 ggcgcgggtt gaaccgctgt cggttcttcc gaagggactc ctcgtacgaa acgtcgatcc
     2161 acagcgccgc ggctcgccgg aggaccgaag ggtccatggc cttgaacgcg gcccgatacc
     2221 cgccgtgctc ggttccgcga gagaactcga tgaccgccgt gccgcgctcc cggaggtcgg
     2281 gctcgtcgtc caggagcttg ccgtactcca gacacaggcg atggatcagc aggtcccata
     2341 gctcgcggcg aaggaagtag ccctcggcgt ccgtgtgcag gcgcggcagc cccatgcgct
     2401 ccaggatggc gtcctcctcg aaccagcccc agatcatggg gaaatcgtcg atcacgcgga
     2461 gctcgccgac gtggaaccgg gccaggcgca tgtccggtgg gagtcggcgc aggtagtcga
     2521 tcacctcgct cttgccggca gccggacgag ccaccaggag aagcacctcg aatcggtcca
     2581 tcgccccatc atagcgcgcc cgcggccatc ccgtcagcgg ccgaggcccg gaggcccgcg
     2641 ggcgttgtcg acgggtgggt gccgacgcgg tcggcggccg ttgtcgacgt tcgacgcctg
     2701 cgctatgatt gccgcaggga ggcgctcgat gagaaaagcg gcgatggtgg tcctggtatc
     2761 gcttcttgcc ctcgctcccc tcggggcgtt cgccgattcc tcgggaacgg tcggcctggg
     2821 cctctccatg ggactcgcgc ttcctttcgg cagcacggcc ggcatcccga gcacggacgg
     2881 gctccccagc ttcaactggg gcttctacgt ggacatcccc ctgatctcca ccttccacat
     2941 caccccttcc tccgagctgt accggttcca gagcacgaac gccacggacg tggacctggc
     3001 gtttaagttc atcgtcccca tctccacgtt cgacgtctac gccggcctgg agccggggct
     3061 gaccgccgtc ggccaggtcc tggacgccca cgtcgggctg gtgggcggcg ccacgctgcg
     3121 cctggtcagc aacctggacg cctttgccca ggccaagtac accgtcgtgt tcgacggaaa
     3181 cgagaacgtg cgggtgctcc acatcaacgc cggcatcctc ttcgcctttt aggccgcggg
     3241 ggtccgtgtc cggtccccgg ggccgcggcc ccggcgtctt gatctactag cttgcaattt
     3301 cttgatatag tgatatagtc aggttcatat cattcccaca tcgacggggg ttcggtaatg
     3361 gcacgcttgg cggccgaggg ggagctctcc gcgcgttgcg cggaaacgct caaggccctg
     3421 gcgcacgcac accggctgcg catcgtagcg atcctctgcg agggcgacga gacggtgatc
     3481 gggttgtccc ggcgcctctc gctcaagcag gccatcgtgt cgcaacagct acggatcctg
     3541 cggatgagcg ggctggtgga tgcctgccgg gtggcggggt tcgcccgcta ccggatcacg
     3601 cagccgcggc tgcggcagct cgtgacgtgt ctggagggct gccacgccgt cgatgtcaag
     3661 agaaggacgg gaggaggagc gcgatgaacg tgaacgtaga ggcggagctg gccaccctga
     3721 aggaaaagct ggaggccatg ccgggcgggg agaacaagct caccatgatc gtgttctccg
     3781 gagggttgga caagcaactg gctgccatga tcatcgccac gggcgccgcg gccatgggca
     3841 tgaaggtgat cctgttcttc accttctggg gaacggccgc ccttcgggac ccggccaagc
     3901 gcgtgggtgg caagagcttc atggggaaga tgttcgggat gatgcttccc aagggccggg
     3961 acaagctcaa gctctcccag atgaacatgg ccggtatggg gacctcgatg ctcaaaggtc
     4021 tcatgcagaa gaagcacgtg gccagcctcg gcgagctcta cgaggtggcc ggacagctcg
     4081 cggtgaagat ctacgcctgc gagatgtcca tggacctcat gggcttcaag cgggaagagc
     4141 tcatcgacta cccggagctc gaggtgtgtg gagtggccac gatgctggcg cacgccaagg
     4201 actcggccat ccatttcttc atctaggagg atctgcgcca tggcggaaca cgacgtcacc
     4261 aagactctgg actacaaggg actgaagtgc ccgatgccgg tcgtgaacat cagcaaggag
     4321 atcggcaagg ttgccgtcgg ccaggtcatc gaggtggaca ccacggatcc gggctccatt
     4381 gccgatttcc cggcgtgggc caagaccatg ggtcatcagg tcctggagat cgcgcaagag
     4441 cccggagtga ttcggatctt cgtgaagcgc ctgaagtagc cggagggtcc catgtctgcc
     4501 caactcctcg cgcaggctgc ggcggcggca agcgctgcta cgtccgcggc ctcgggcgcg
     4561 gcggccgccg gcgcgccggc gacggct
//
LOCUS       DASRZS010000649         5728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2277142, whole genome shotgun sequence.
ACCESSION   DASRZS010000649 DASRZS010000000
VERSION     DASRZS010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2277142"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VFH83_15825"
     CDS             complement(<1..762)
                     /locus_tag="VFH83_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter TolR"
                     /protein_id="HET6487897.1"
                     /translation="MATKAGAFDIVGDVGTQRIPGSTVYHPEDQTYTITGGGANTWGT
                     VDEFHLAALRVRGDFILTAEGSLVGKGVQAHRKWGLTIRERWEGSSLHADAAIHGDGL
                     TSLQYRERQGAETLEVKAAMTHADVVQLERSGQTITMRAAKQGEPLVETGKVSMAFSD
                     EAFVGIFICSHDISITEKAVIRNVRLDVAAGPGVDGDKVPSPSRLEVLDVETGLRRVL
                     YTSRAHFEAPNWSRDGTYLLYNQEGRIYRLPLDSRV"
     gene            complement(820..1683)
                     /locus_tag="VFH83_15830"
     CDS             complement(820..1683)
                     /locus_tag="VFH83_15830"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6487898.1"
                     /translation="MKIGIDSYCYHRFFGEVYDQQKKPSRQMTLEDFIRRAHSLGVDG
                     VSLESCFIPSFDAAYLSQVKAMLDQYGLDRVFAWGHPDGLEGGRNERAFDEMVKSLEY
                     AKAIGATVMRVVGASHALRKEPHGPMLEKLAKMFRAAAKAAEAAGAKLADENHIDFTS
                     DEMLSLIKDVDSPYFGINFDTGNFMRLLDDPVKGMQKLARHVYSTHIKDLKINRQAAV
                     DDWYFFSTAPVGDGLVDNRKLAELLRDAGYRGFLAVELDFLHPDYGDDEDRAVARSVK
                     ELKRIARAVGG"
     gene            complement(1715..3034)
                     /locus_tag="VFH83_15835"
     CDS             complement(1715..3034)
                     /locus_tag="VFH83_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487899.1"
                     /translation="MKARLVPVHFGAEMDDAFRQHLDIVTKLFAEEAEVLPPVALGAR
                     LPPADAVVFPQLVGQAFRQARALKRLTLPVLVLTSDFGTVNMWDWEIVTFLKDLGMKV
                     FTPYDPGLTRVICRALALRREMRTMRYLVFQDRPGVGGEQAEIFKRFWWWEEQATDLI
                     RQRFGIQIVQKSFQKLGADAKAIGDADARAALKDRPVPTKGLAGRQMLSAIKLYLAVR
                     RELDADPSIQGAGINCLNESAFSDTTPCLLWDLLYQERRLIWACEADTLSLLTTMLAD
                     RALQAPVMMSNLYPFLMGAAALKHEKIESFPDVKEPENHLLMVHCGYFGVVPRPFCVS
                     WNLRPKVLAIVDPNSAVIDARMKAGPATLAKLHPQLSSLQLIDAELEGYVQYPGSDCR
                     NGGLLRVRDGKALMKSFYSHHYCLLTGRRTTELELVARVMDLEAVAL"
     gene            complement(3031..4203)
                     /locus_tag="VFH83_15840"
     CDS             complement(3031..4203)
                     /locus_tag="VFH83_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6487900.1"
                     /translation="MKTLNIGIVGYKFMGKAHSNAWRQASVFFDPEARPVLKVACGRH
                     RESLEEFAHRWGWERTETDWRKMVQSDDVDVVDVSAPQYLHYDVVMEALRAGKHVFCE
                     KPLALSAAQAREMYELAEKKGVTHYVNHNYRRAPAVRLARRLIDEGRIGRIFHWRGSY
                     LQSWIVDPGFPLTWQLQKELAGSGPQGDLNSHSVDLARYLVGDIRKVTGMTANFITER
                     PLSDDAASGALSGKVRGGSEETPTGKVTVEDAAFMVVEFANGALGSFAASRFAPGRKN
                     YNQFEIYGSKGSILFDLERMNELQFFSQDDPAHAQGFRTIIATEGDQHDYVGNWWPAG
                     HIIGYEHEFVHAVVDFVHAVVTGTKIEPSFYDGWKELEVLEAGLLSARTGRAVELS"
     gene            complement(4243..5136)
                     /locus_tag="VFH83_15845"
     CDS             complement(4243..5136)
                     /locus_tag="VFH83_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6487901.1"
                     /translation="MKASVPRRIFLVCNTVFLLVVALVCIAPFINLLAVSFSNKVAVS
                     GGEVTFYPIGFTLMSYQFITGTSKFVQSVLVSLERTAIGLPINLLLIILTAYPLSKSR
                     KDFRARNVFSWFFVVTILFNAGLIPSYMIVRYTGLIDHLWALILPGALPVFSMLVVMN
                     YLRSLPKELEEAAYIDGASHLQTLIRIVLPVATPTIATVALFSFVGHWNSWFDGMIYM
                     NKVQHYPLQTYLQTVVVNPEEFFRNNTDRSGNLAKYLALLSARTTGAAQLFLAMVPIL
                     CVYPYLQRYFTTGLVLGSVKG"
     gene            complement(5151..>5728)
                     /locus_tag="VFH83_15850"
     CDS             complement(5151..>5728)
                     /locus_tag="VFH83_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6487902.1"
                     /translation="QTLVYLPNFLSWVILAIVLVDILSPSEGVVNVLLSKVGIKPIFF
                     LGDARVFPITMIVTDIWKGFGFGTIIYLAALTSIDPGLYESAMMDGANRWQQTWHITL
                     TGISSTIVLLSVLSLGNILNGGFDQIYNLYSPVVYSTGDILDTLVYRLGIQQAQYSIS
                     TAAGLFKSAVSLVFIVASYYLADRYAGYRVF"
BASE COUNT         1018 a         1883 c         1808 g         1019 t
ORIGIN      
        1 gactcggctg tccaggggaa gccgatagat cctgccctcc tggttgtata acaggtacgt
       61 gccgtcccgc gaccagttgg gtgcctcgaa gtgggcgcgg ctggtataga ggacgcgacg
      121 gagacccgtc tcgacatcca gcacctcgag ccggctgggc gacgggacct tgtcgccgtc
      181 cacgcccggg ccggcggcta cgtcgagacg gacgtttcgg atgaccgctt tctcggtgat
      241 cgatatgtcg tgggagcaga tgaagatccc gacgaacgct tcatcggaaa acgccatcga
      301 gaccttcccg gtttctacga gcggctcacc ctgcttcgcc gcgcgcatcg tgatggtctg
      361 cccggaacgc tcgagctgca cgacatcggc gtgcgtcatc gcggctttca cttcgagggt
      421 ttccgccccc tgcctctccc ggtactgcag ggaagtgagc ccgtccccgt ggatggccgc
      481 gtccgcgtgc agggagcttc cctcccatcg ctcgcggatc gtcagacccc atttccgatg
      541 cgcctgcact cccttcccta ccagacttcc ttcggccgtc aggatgaagt ccccccgcac
      601 cctgagggcc gccaggtgga actcatccac cgtgccccac gtgttggctc cgccgcccgt
      661 gatcgtgtac gtctgatcct ccgggtggta gaccgtgctc ccggggatgc gttgggtgcc
      721 gacgtccccg acgatgtcaa aagcccctgc cttggtggcc atgcccaatt ctcctcagtc
      781 gttctccagg atctccgtca gaaccatcgc gcccctgggt cacccgccca cggctcgcgc
      841 gatccgcttc agctccttga cgctccgcgc caccgcgcgg tcctcgtcat ccccgtagtc
      901 cgggtgcagg aagtccagct ccacggccag gaagccccgg tagccggcgt cccgaagcaa
      961 ctccgccagc ttgcggttgt ccacgagccc atccccaacg ggcgccgtgg agaagaagta
     1021 ccagtcgtcc acggcggcct ggcggttgat cttcaggtcc ttgatgtgcg tcgagtatac
     1081 gtgccgtgcc agcttctgca tccccttcac cgggtcgtcc aggagcctca tgaaattgcc
     1141 ggtatcgaag ttgatcccaa agtacggcga gtccacgtcc ttgatgaggg agagcatctc
     1201 atccgaggtg aaatcgatgt ggttctcgtc cgccagcttt gcgccggccg cctccgccgc
     1261 cttcgccgcc gcgcggaaca tcttcgccag cttctccagc atggggccgt gcggctcctt
     1321 gcgaagggcg tgactcgcgc ccacgacccg catcaccgtg gccccgatgg ccttcgcgta
     1381 ctccaggctc ttcaccatct cgtcgaaggc gcgctcgttt cggcccccct ccagcccgtc
     1441 cggatggccc caggcaaaca cccgatccag gccgtactgg tccagcatag ccttcacctg
     1501 ggacaggtag gcggcatcga agctggggat gaaacaggac tccagggaga cgccgtctac
     1561 ccccagggag tgcgcccgac ggatgaagtc ctccagggtc atctgccggg agggcttctt
     1621 ctgctggtca tagacctcgc cgaaaaaccg atggtagcaa tagctgtcga tcccgatctt
     1681 catcacaacc ctcccgaggc ctcggtgtcc ggcctcacaa cgcgaccgcc tcgaggtcca
     1741 tgacccgcgc caccagctcg agctccgtgg tacgcctccc cgtcaggagg cagtagtggt
     1801 gggagtagaa ggacttcatc agcgccttcc cgtcccgaac gcgcagcagg ccgccgttcc
     1861 tgcagtccga gccgggatac tggacgtagc cttccagctc cgcgtcgatg agctgcaggc
     1921 tcgatagctg gggatgcagc ttggccaggg tggcgggacc cgccttcatc cgggcgtcga
     1981 tcacggcgga gttgggatcc acgatggcca gcaccttggg ccgaaggttc caggaaacgc
     2041 agaagggacg cggcaccacg ccgaagtagc cgcagtggac catgagcaga tggttctccg
     2101 gctcctttac gtcggggaag ctctcgatct tttcgtgctt gagggcggcc gcgcccatga
     2161 ggaacgggta gaggttgctc atcatcacgg gcgcctgcag ggctcgatcc gcgagcatgg
     2221 tcgtcagcag ggaaagggtg tccgcttcgc acgcccagat gaggcgccgc tcctggtaga
     2281 gcaggtccca gagcagacac ggcgtggtgt ccgagaaggc ggactcgttc aggcagttga
     2341 tccccgctcc ctggatggac ggatccgcgt ccagctcccg ccgcacggcc aggtacagct
     2401 tgatggcgga aagcatctgc cgccccgcca gccccttggt cggcaccggc cggtctttga
     2461 gggcggcccg ggcatccgcg tcaccgatgg ccttggcgtc cgcgccgagc ttctggaagc
     2521 tcttctggac gatctggatg ccgaagcgct gccggatcag gtcggtggcc tgttcctccc
     2581 accaccagaa ccgcttgaag atctccgcct gctccccgcc caccccggga cggtcctgga
     2641 acacgagata ccgcatcgtg cgcatctcgc gtcggagcgc cagcgcccgg cagatcacgc
     2701 gcgtgagacc cgggtcgtac ggggtgaaga ccttcatgcc caggtccttc agaaacgtga
     2761 cgatctccca gtcccacatg ttcaccgtcc cgaagtccga ggtgaggacg aggaccggga
     2821 gggtcagacg tttcagcgcg cgagcctgcc ggaacgcctg gccgacgagc tgggggaaga
     2881 cgacggcgtc cgccgggggc agcctggccc ccaacgccac gggcggcagc acctctgcct
     2941 cctcggcgaa cagcttcgtc acgatgtcca gatgctgacg gaacgcgtca tccatctccg
     3001 cgccgaaatg gacggggacc aacctcgcct tcatgacagc tccaccgccc tgccggttcg
     3061 cgccgagagc agtccggcct ccagcacttc gagctccttc cacccgtcgt agaagctcgg
     3121 ttcgatcttc gtcccggtga ccacggcatg gacgaaatcc acgacggcgt gcacgaactc
     3181 gtgctcgtac ccgatgatgt ggccggccgg ccaccagttg ccgacgtagt cgtgctggtc
     3241 gccctcggtg gcgatgatcg tacggaaccc ctgcgcgtgg gcggggtcgt cctgggagaa
     3301 aaactgcagc tcgttcatcc gctccaggtc gaagaggatg ctccccttgc tgccgtagat
     3361 ctcgaactgg ttgtagtttt tgcggcccgg cgcgaagcgg ctggccgcaa aggaaccgag
     3421 cgccccgttg gcgaactcca cgaccatgaa ggccgcgtcc tccacggtca ccttccccgt
     3481 cggcgtctct tccgagccgc ccctgacctt gccgctgagg gcgccggacg ccgcatcgtc
     3541 ggacaggggc cgctccgtga tgaagttggc ggtcatcccc gtcaccttgc ggatatcccc
     3601 taccaggtaa cgggccaggt ccacgctgtg cgagttcagg tcaccctggg ggcctgaacc
     3661 ggcgagctcc ttctgcagct gccaggtgag cggaaagccc ggatccacga tccaggactg
     3721 caggtagctc ccccgccagt gaaagatccg accgatgcgt ccctcgtcga tgagacgccg
     3781 ggccaggcgg accgccggag ctcgccggta gttgtggttc acgtagtggg tgaccccctt
     3841 cttctccgcg agctcgtaca tctcccgggc ctgcgccgcg ctcagggcca gcggcttctc
     3901 gcagaagacg tgcttgcccg ctcgcagggc ctccatgacc acgtcgtagt gcaggtactg
     3961 gggggcgctc acgtccacga cgtccacgtc atcggactgg accatcttcc tccagtcggt
     4021 ctccgtgcgc tcccagcccc accggtgggc gaactcctcc agggactccc gatgccggcc
     4081 gcaggccacc ttgagcaccg gcctggcctc cgggtcgaag aacaccgacg cctggcgcca
     4141 ggcgttggag tgggctttgc ccatgaactt gtaccccacg ataccgatgt tgagagtctt
     4201 catttcgtcc tttcaggccc gaaggctttc ggctccgtgg gcctatccct tgacgctgcc
     4261 caacaccagg ccggtcgtga agtacctctg gaggtacgga tagacgcaca ggatggggac
     4321 catggcgaga aacaattgcg cggcccccgt ggtccttgcg ctgagaaggg ccaggtactt
     4381 ggccaggttg ccgctgcgat ccgtgttgtt gcggaagaac tcctcgggat tgacgacgac
     4441 cgtctgcagg taggtctgca gcgggtagtg ctggaccttg ttcatgtaga tcatgccgtc
     4501 gaaccagctg ttccagtggc ccacgaagga aaagagcgcg accgtggcga tggtgggcgt
     4561 cgcgaccggc aagacgatcc ggatcagcgt ctggaggtgc gaggcgccgt cgatgtaggc
     4621 ggcctcctcc agctccttcg gcaggctgcg aaggtagttc atcaccacca gcatgctgaa
     4681 gacgggaagc gcgccgggca ggatgagcgc ccagaggtga tcgatcaacc cggtgtaccg
     4741 cacgatcatg tacgagggga tcaaccctgc gttgaagaga atggtcacga cgaaaaacca
     4801 cgagaacacg ttgcgggccc ggaagtcctt ccgcgacttg gacagcgggt aggccgtcag
     4861 gatgatcaga agcaggttga tgggaagtcc gatggcggtc ctctccaggg agaccagcac
     4921 ggactggacg aacttcgagg tgccggtgat gaactggtag gacatcagcg tgaacccgat
     4981 cgggtagaag gtgacctctc cgccggacac ggcgaccttg ttggagaatg agaccgccag
     5041 caggttgatg aacggcgcga tgcatacgag ggccacgacc aggaggaaga ccgtgttgca
     5101 gacgaggaag atccttcgcg gcacggatgc cttcacgctc ccctcctccg ctagaagact
     5161 cggtacccgg cataccggtc ggccaggtag tacgacgcga cgatgaagac cagcgagacg
     5221 gccgacttga agagacccgc tgccgtggag atggagtact gcgcctgctg gatgcccagc
     5281 cggtagacga gcgtgtccag gatgtctccg gtggaataga ccacggggct gtacaggttg
     5341 tagatctggt cgaaccctcc gttcaggatg ttgcccaggc tcagcacgct cagcagcacg
     5401 atcgtgctgg agatgcccgt cagggtgatg tgccaggtct gctgccagcg gtttgctcca
     5461 tccatcatgg ctgactcgta gagcccgggg tcgatgctgg tgagagccgc cagatagatg
     5521 atggtcccga agccgaagcc cttccagatg tccgtcacga tcatggtgat gggaaacacc
     5581 ctggcgtcgc ccaggaagaa gatcggcttg atcccgacct tggagagaag cacgttcacc
     5641 actccctcgc tgggggagag aatgtcgacc aggacgatgg ccaggatgac ccaggaaagg
     5701 aaattgggga gatacacgag ggtctgga
//
LOCUS       DASRZS010000650         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2684037, whole genome shotgun sequence.
ACCESSION   DASRZS010000650 DASRZS010000000
VERSION     DASRZS010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2684037"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2262
                     /locus_tag="VFH83_15855"
     CDS             <1..2262
                     /locus_tag="VFH83_15855"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET6487903.1"
                     /translation="IEGDFREENPCGGGGGYLPCGIGWYRKSFRLPDDWAGRLVSIEL
                     DGVYKNSDVWINGEHLGFQPYGYTSFSYDLTAHLRPATAANLLAVRVDNRLLPDSRWY
                     TGSGVYRHTWLTGTDSLHVPQWGVFVATASVDPEEVEVLARTTLRNDSSVDRDCEVHS
                     AIADSSGRIVGSAHSPQLVPVGTDQEVRHRIRLAQPRRWSLEDPHRYSLRTTIRQEGR
                     VVDEVVTPFGVREILFDADRGFLLNGQKRKILGVCLHHDGGCVGAAVPEGMWERRLRI
                     LKAMGCNAVRTSHYPPAPELLDLCDRMGFLVMDEAFDEWRIGKFAHGYHEYFDEWAER
                     DLRAMIRRDRNHPSIVLWSVGNEIPEQTQPEGEAILKRLVDVARAEDPTRLVTSACDN
                     IAAHVPTRREFTDLLDVVGYNYVDRWEDRRERYYSVDREDFPRRRMIGSENLSLSGVR
                     GSTGAEDPTLVWGLSPVRPVAVEQLWRFTRIHDYVAGDFLWTGIDYLGETRWPHKNAS
                     CGVLDLCGFPKDGYYFYQSQWTEQPMLHLFPHWTWPGREGEIVAVLCFTNCESVELFL
                     NGRSLGEKSYAFPLRGMDRSRTWDDQVRDPVRRPTTGDLHLAWDVPYQAGTLRAVGRR
                     QGKEVCVEELRTASDPQRLVAELEPSWIRADGRDVCHVTVRVCDGQGVLCPEADSLVR
                     FSVEGAARLIGVDNGRPDSHERFQAKERHAFRGLCLAVVQSTGAAGTILIQVSSEGLE
                     PSAVELRAGEAKA"
     gene            2259..>2600
                     /locus_tag="VFH83_15860"
     CDS             2259..>2600
                     /locus_tag="VFH83_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487904.1"
                     /translation="MIRPFGFGKDKVFTGALDFSFLLDAPAGKHGFLQIRDGHFSVGE
                     RRIRFYGFNLPFGCPFCPHADAEIIAERLARAGVNFARIHAVDFRPRAGPSPLIDYAQ
                     GTSRAFDPVQWD"
BASE COUNT          442 a          829 c          868 g          461 t
ORIGIN      
        1 atcgagggcg atttccggga ggaaaacccc tgcggtggag ggggcggcta tcttccctgc
       61 gggatcgggt ggtaccgcaa gtccttccgc cttccggacg actgggcggg gaggctcgtc
      121 tccatcgagc tggacggcgt ctacaagaac agcgacgtct ggatcaatgg ggagcatctg
      181 ggtttccaac cgtacggata cacgagcttc tcctacgacc tgacggcgca cctcaggccg
      241 gcgacggccg ccaacctcct ggcagttcgg gtggacaacc gtctccttcc tgactctcgc
      301 tggtataccg gctcgggtgt ttaccgccac acctggctca ccgggaccga ctctctccac
      361 gtcccccaat ggggagtctt cgtcgcgacc gcttcggtcg accccgagga ggttgaggtc
      421 ctggcaagaa ccacgctgcg caacgacagc agcgtggacc gagactgcga ggttcatagc
      481 gccatcgcgg attcgtcggg gcggatcgtc gggtcggcgc acagccctca gctcgtgcct
      541 gtcggaacgg accaggaagt acggcatcgg atccgcctgg cgcagccccg acggtggtct
      601 ctggaggatc cccatcggta cagcctgcgc accaccattc ggcaggaggg tcgcgtcgtt
      661 gacgaggtgg tcacgccttt cggggttcgg gagatcctct tcgacgcgga ccgagggttc
      721 ctgctgaacg gccagaagcg caagatcctg ggagtctgtc tccaccacga cggcggatgc
      781 gttggggcgg cggttcccga aggcatgtgg gagcgcagac tgcggatcct gaaggccatg
      841 ggctgcaacg ccgtcaggac cagccactat ccgccggcgc ccgagctcct ggatctctgc
      901 gaccgcatgg ggttcctcgt catggacgag gcgttcgacg agtggaggat cggcaagttc
      961 gcccatgggt atcatgagta tttcgacgaa tgggcggagc gcgatcttcg ggccatgatc
     1021 cgacgggacc gcaaccaccc cagcatcgtc ctgtggagcg tgggaaacga gatccccgag
     1081 cagacccagc cggaaggcga ggccattctg aagaggctcg tggacgtggc tcgtgccgag
     1141 gacccaacca ggctcgtgac ttccgcttgt gacaacatcg ccgcccacgt tccgacccgg
     1201 cgggagttca ccgacctgct ggacgtcgtc ggctacaact acgtggatcg gtgggaggac
     1261 cggcgggagc gctactactc tgtcgaccgg gaggacttcc cccgtcggcg gatgatcgga
     1321 agcgagaatc tctctctttc cggcgttcga ggatccaccg gcgcggagga tccgactctc
     1381 gtctggggcc tgtctcccgt tcgccctgtc gctgtggagc aactctggag attcacccgg
     1441 atccacgact acgttgccgg agatttcctc tggaccggaa tcgactacct cggagagacc
     1501 cggtggccgc acaagaacgc gagctgcggc gtactggacc tctgcgggtt tcccaaggat
     1561 ggatactact tctaccagag ccagtggact gagcaaccca tgctccatct cttcccccac
     1621 tggacgtggc cgggcagaga aggagagatc gtcgcggtcc tctgcttcac caactgcgaa
     1681 tccgtggagc tctttctcaa cggccgctcc cttggcgaga agagctacgc ctttccctta
     1741 cggggcatgg atcggtcgcg gacctgggac gaccaggtgc gagacccggt gaggcggcct
     1801 acgaccgggg acctccatct tgcctgggac gtgccttacc aggccgggac gcttcgcgcg
     1861 gtcggacggc gccagggcaa agaggtctgc gtcgaagagc tccgcaccgc gagcgatccg
     1921 caacggctgg ttgcggagct ggagccatcc tggatccggg cggacggcag agacgtctgc
     1981 cacgtgaccg tgagggtgtg tgacggtcaa ggcgtcctgt gccccgaggc ggactccctg
     2041 gtccggtttt ccgtggaggg agccgcgcgg ctcatcgggg tggacaacgg tcggccagac
     2101 agccacgaga gattccaggc gaaggagcgg cacgcctttc ggggactgtg cctggcagtc
     2161 gtgcaatcta cgggagcggc tggcacgatc ctcatccagg tatcatcgga gggactcgag
     2221 ccgagcgcgg tggagcttcg cgccggggag gcgaaagcat gatccggccg ttcgggttcg
     2281 gcaaggacaa ggtcttcacg ggggccctgg acttcagctt tctcctggat gcgccggccg
     2341 gcaagcacgg attcctccag atccgagacg gccacttctc cgtgggggag cgacggatcc
     2401 gcttctacgg cttcaacctt cccttcggct gccccttctg tccccacgcg gatgccgaga
     2461 tcatcgccga gcggctggct cgagccgggg tgaacttcgc caggatccac gcggtggact
     2521 tccgtccgcg ggccggtccg tcccccctca tcgactacgc tcagggtacc tcccgcgcgt
     2581 tcgacccggt gcagtgggac
//
LOCUS       DASRZS010000651         4973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3835302, whole genome shotgun sequence.
ACCESSION   DASRZS010000651 DASRZS010000000
VERSION     DASRZS010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3835302"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /gene="dnaG"
                     /locus_tag="VFH83_15865"
     CDS             complement(<1..871)
                     /gene="dnaG"
                     /locus_tag="VFH83_15865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET6487905.1"
                     /translation="MRIPDSTLSEIRDRVDIAEVIGEHVSLQRRGSRFWGLCPFHQEK
                     TPSFSVTPERGVFYCFGCHKGGTIFDFVMETEKVPWRDAVQMLARKAGIELPQDDPDP
                     DGLPREAFLELHRRVAGSFHWLLTEGQQAAAARQYLESRGVTGESIEAFQLGYAPPDR
                     QWLHGFLVQKGYSPEFLARTGLFVEGSREQAMALFGNRIMFPIANARGEVLAFGGRAL
                     GSGQPKYLNSPETAFFRKGEHLFGMDRAAQAIRTDGFFVLVEGYMDVIAMHQAGFSAA
                     VAPLGTSLTDRQAR"
     gene            complement(876..1904)
                     /gene="mltG"
                     /locus_tag="VFH83_15870"
     CDS             complement(876..1904)
                     /gene="mltG"
                     /locus_tag="VFH83_15870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET6487906.1"
                     /translation="MSKAVRYLIRGAILAAAVLVVLAAGIAVVNSSPARGRQGADRLF
                     TIGRGENLSRISRELKAQGFIRSALFLDVLGRLRGTQGAFKAGSYRIPAGASTRSVHN
                     LLVQGAESMDKVTIPEGWTASKIARHLEALGVCPASDFEAAVHSSSTAAELGIKGDSL
                     EGYLFPDTYLVPRPFPATAMVRLMVGTFFDTVGVLDPGWKEMGPSSLRDTVILASIVE
                     REYQVADEAPVIASVFNNRLKRNIGLESCATLEYIITEIQQKAHPEYITIADEKIDSP
                     YNTYKWAGLPPGPISNPGRVALEAAFHPARTDYLYFVLRDPEAGRHYFSRNLNEHNQA
                     KYLYLKKP"
     gene            complement(1901..>4973)
                     /locus_tag="VFH83_15875"
     CDS             complement(1901..>4973)
                     /locus_tag="VFH83_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET6487907.1"
                     /translation="LWTFGAGVDVPLGVRDTQPPTITLETDKSFSISPNHDGVQDDLM
                     LPVSITDNRYVVGYRFIISDSAGAAIRTIQNKEDRPENRDFQSILARLTYVKTGISIP
                     KSLRWDGTSDAGTVVPDGTYHYRVEAWDDNGNLARSPDGTVVVDDTPPSVSASASYLI
                     FSPDGDSVRDTLPIHQTGSTEDLWSGTISSINGSIVRSYLWRDSAPADFEWDGKTDDG
                     QSAPDGVYSYRIEATDRAGNKGSAEIDNVIIDTRPTPVQLSIDLSFFSPNGDGVKDVV
                     TFSPVVPVATGVESWTLTVFDAQGSARRSIVGKLAVPPSIVWDGKDDEGKILPEGVYR
                     AAFAVRYTNGHNPTAQSPDITIKLTPPRAAAKAQYDVFSPGGARSTDAIYQDTSEELF
                     WTGIISDAKGNELKTMVWRGRADDQWVWDGRGDDGNPLPDGTYTYTLSATDRAGNTGS
                     STPIAVRIDTQATPVRLTVDPQYFAPNGNGTRDRVRLIPTVRVTTGIDSWTYAIKNAK
                     GDVVRTFVGGSRPPDVVSWMGLDDAGQRLPDGAYSATLTVTYQNGNVSKAESGPVSID
                     THTPTISVSSDALLFSPTADSKLNAVTIKQSSSSEDLWEGEIRNSAGQRVRGWFWKGV
                     AAPFSWDGKDDNGNLAADGYYSYFVKAQSRGGITTTAELKGIQIDSRPTPVFVTAGSN
                     GFSPNGDGVRDTISFAILATLREGIRSWKLSLLHESQGEQRSFSGTGPIPTSVVWDGK
                     NQAGIQTAPDGLYTAVLHVEYYKGNVAEAKSTAFRLAVTPPAVDLAIGQLPFSPDNDG
                     VNDELTISLNVNDPVPIDSWTVTILDPQNHPFARFTGKGAPSQTIIWNGLSDSGELVQ
                     SAEDYPMTFAIRDELGNATTVGKTIPVDVLVVRDGDQLKVRIASITFAADSPDYVNVD
                     PDKAQRNAQTIARLAEIFKKYSQYKIEIQGHANLVNYTDPAKAKVEQEQQLIPLSKKR
                     ADAIRDALITQGIDGSRITTVGIGAADPLVPFSDLDNNWKNRRVEFILVRQ"
BASE COUNT          826 a         1621 c         1594 g          932 t
ORIGIN      
        1 ggcgcgcctg acgatccgtc agagacgtac ccagcggagc gaccgcagcg gaaaagcccg
       61 cctggtgcat ggctatcacg tccatgtagc cttccaccag gacgaaaaag ccgtcggtcc
      121 gaatggcctg ggccgcccgg tccatcccga agaggtgttc ccccttgcgg aagaatgccg
      181 tctcgggaga gttcaggtac ttgggctgac ccgaccccag tgcccgccct ccgaacgcca
      241 gcacctcccc cctcgcgttg gcgatgggga acatgatccg gttcccgaag agcgccatcg
      301 cctgctctcg ggacccctcg acgaagagcc ccgtcctggc caggaactcc ggagagtacc
      361 ccttctggac caggaacccg tgcagccact gccgatccgg cggagcgtag ccgagctgga
      421 acgcctcgat agactccccg gtcactccgc ggctctccag gtactggcgg gccgcagccg
      481 cctgctggcc ctcggtgaga agccagtgga agctcccggc cacccgccga tgcagctcca
      541 ggaatgcctc tcgagggagg ccgtccggat ccgggtcatc ctggggaagc tcgatgccgg
      601 ccttgcgggc cagcatctgc acggcgtccc gccaggggac cttctcggtc tccatgacga
      661 agtcaaagat cgtgccgcct ttgtggcagc cgaagcagta gaaaactccc cgttcgggtg
      721 tcacgctgaa cgagggcgtc ttctcctggt gaaacgggca cagaccccag aaccggctgc
      781 cgcgccgctg cagggagacg tgctcgccga tcacctcggc gatatctaca cggtcgcgaa
      841 tctccgagag cgtgctgtcc ggtatcctca tacccctacg gcttcttaag atagaggtac
      901 ttcgcctgat tgtgctcgtt cagattgcgg gagaagtagt gacgacccgc ctctgggtcg
      961 cgcaaaacga agtacaggta atcggtccta gccggatgaa acgcagcttc caacgccacc
     1021 ctccccgggt tcgagatcgg tccgggaggc aatcccgccc acttatacgt attgtacggc
     1081 gagtcgatct tctcatcggc aatggtgatg tactccggat gcgccttttg ctggatctcc
     1141 gtgataatat attcaagcgt cgcgcatgat tcaagcccta tgtttcgctt cagacgatta
     1201 ttgaagaccg aggcgatcac gggcgcctcg tccgctacct gatactcccg ctcgacgatg
     1261 gacgccagga tgaccgtatc ccgcagggaa gagggtccca tctctttcca gccgggatcc
     1321 agaacgccaa ccgtgtcgaa gaacgtgccc accatcaacc ggaccatcgc cgtcgcagga
     1381 aagggcctgg ggaccaggta cgtatcggga aagagatacc cctccaggct gtctcctttg
     1441 attccgagct ccgcggcggt cgaagaagaa tgcacggcgg cctcgaaatc cgaggcgggg
     1501 cagaccccca gagcctccag gtgacgggca atcttgctgg ccgtccatcc ctccgggatg
     1561 gtgaccttgt ccatgctctc cgcgccctgg accagcaggt tgtggacgga ccgcgtcgac
     1621 gcgccggctg gaatccggta ggagccggcc ttgaaggcgc cctgggtccc gcgcaggcgc
     1681 cccaacacat ccaggaacag ggcggagcgg atgaatccct gggccttcag ctctcggctg
     1741 atccgcgaca ggttctctcc ccttccgatc gtgaacaaac ggtcggcccc ctggcggccc
     1801 cttgcggggc tggagttcac cacggcgatg cccgccgcca acacgacgag cacggcggca
     1861 gccaggatgg caccgcggat gagatatcgg acggccttgc tcattgtcga accaggataa
     1921 actccacccg ccggttcttc cagttgttgt ccaggtcgct gaaaggcacc aggggatccg
     1981 cggctccgat tcccacggtc gtgatccggg agccgtcgat gccctgcgtg atcagggcat
     2041 ctcggatcgc gtccgcccgc ttcttggaga gcgggatgag ctgctgctcc tgctccacct
     2101 tggccttcgc cgggtccgtg tagttcacga ggttggcgtg gccctggatc tcgatcttgt
     2161 attgggagta cttcttgaag atctcggcca gccgcgcgat ggtctgggcg ttccgctgcg
     2221 ccttgtcggg atccacgttc acgtagtccg ggctgtccgc cgcgaaggtg atgctggcga
     2281 tgcgcacctt gagctggtcc ccatcgcgga ccaccagcac gtccaccggg atggtcttgc
     2341 cgaccgtcgt ggcgttgccc agctcgtccc ggatggcgaa cgtcatgggg tagtcttcgg
     2401 ccgactgcac cagctcgccc gaatccgaca ggccgttcca gatgatggtc tgggacggag
     2461 cccccttgcc cgtgaagcgc gcgaagggat ggttctgcgg gtccaggatc gtgacggtcc
     2521 acgagtcgat ggggacgggg tcattcacgt tcagggagat ggtgagctcg tcattcacgc
     2581 cgtcgttgtc cggggagaac ggcagctgcc caatggccag gtccacggcg ggaggtgtca
     2641 cggccagtcg gaaggccgta gacttcgcct cggccacgtt gcccttgtag tactcgacgt
     2701 gcaataccgc cgtgtacaat ccatccggcg cggtctggat ccccgcctgg ttcttgccgt
     2761 cccagaccac cgaggtcggg atgggaccgg tcccggagaa gctccgctgc tccccctggc
     2821 tttcgtgcag cagggagagc ttccaggacc ggatcccctc ccgcagtgtc gccaggatgg
     2881 cgaagctgat cgtgtcccgc accccgtctc cgttcggtga gaaaccgttg gagccggcgg
     2941 tcacgaagac cggcgtcggc cggctgtcga tctggatccc cttgagctcc gccgtggtgg
     3001 tgattccacc tcgggattgc gccttcacga agtaggagta gtagccgtcc gcggccaggt
     3061 ttccgttgtc gtccttcccg tcccaggaga agggcgcggc aacgcccttc cagaaccagc
     3121 cccgaacacg ctggcccgcg ctattgcgga tttcaccttc ccaaaggtcc tccgagctgg
     3181 agctctgctt gatggtgacg gcgttgagct tgctgtccgc tgtcggggag aacagcaggg
     3241 catcggagct cacgctgatg gtcggcgtgt gggtgtcgat ggagaccggc ccgctttccg
     3301 ctttggagac gttcccgttc tggtacgtga cggtgagcgt cgcgctgtac gctccgtcgg
     3361 gaagccgttg accggcgtcg tccagtccca tccaggacac gacgtcgggg ggcctgcttc
     3421 ctccgacgaa tgtcctgacc acgtcgccct tggcgttctt gatcgcataa gtccaggaat
     3481 cgatccccgt ggtcacacgg acggtgggga tcagccgcac ccggtccctg gtgccgttgc
     3541 cgttgggggc gaagtactgc gggtccacgg tgagccgcac gggggtagcc tgggtgtcga
     3601 tgcggaccgc aatgggcgtc gagcttcccg tattgcccgc gcggtcggtc gcgctgaggg
     3661 tgtaggtgta ggtgccgtcc ggaagcggat tgccatcgtc cccgcggccg tcccagaccc
     3721 actggtcgtc cgccctgccc ctccagacca tggtcttcag ctcgttgccc ttggcatcgg
     3781 agatgatccc ggtccagaac agctcctccg aggtgtcctg gtagatggcg tccgtgctgc
     3841 gggcgccacc gggagagaac acgtcgtatt gagccttggc cgcggcgcgc ggcggtgtca
     3901 gcttgatcgt gatgtcgggc gactgcgcgg tcgggttgtg gccgttcgtg taccgcacgg
     3961 cgaacgccgc tcgatacacc ccttcaggca ggatcttgcc ctcgtcgtcc tttccgtccc
     4021 acacgatcga aggagggacc gccagcttgc ccacgatact tcgccgggcg ctgccctggg
     4081 cgtcgaaaac ggtgagcgtc cagctctcca cgccggtggc gaccggcacg accggggaga
     4141 aggtgacgac gtccttcacc ccgtccccgt tgggggagaa gaaagaaagg tcgatggata
     4201 gctgcacggg cgtggggcgc gtatcaataa tgacgttgtc gatctccgcc gaccccttgt
     4261 tgcccgcccg atccgtagcc tcgatccggt agctgtagac gccgtccggc gccgactgac
     4321 cgtcgtccgt cttgccgtcc cattcgaagt ccgcgggcgc cgaatctctc cagaggtacg
     4381 agcggacaat agagccgttg atgctgctga tcgtcccgct ccagaggtcc tcggtggaac
     4441 cggtctggtg aatgggcagc gtatcccgca cgctgtctcc gtcgggggag aagatcagat
     4501 acgacgcgct ggccgacacg gaaggcgggg tgtcatccac cactacggtc ccgtccgggg
     4561 aacgcgccag gttcccgttg tcgtcccacg cctccacccg gtagtggtag gtcccatccg
     4621 ggacgaccgt cccggcatcc gacgtgccgt cccaccgcag cgacttgggg atcgagatgc
     4681 ccgtcttcac gtaggtcagg cgggcaagga tgctctggaa gtcgcggttc tccggtcgat
     4741 cctccttgtt ctgaatggtc cgaatggccg cgcccgcgga atccgagatg atgaagcggt
     4801 accccacgac gtaccggttg tcggtaatgc ttaccggcag catcaggtcg tcctgcaccc
     4861 cgtcgtggtt cggcgagatg gagaaggact tgtcggtctc cagggtgatg gtgggcggct
     4921 gggtatcccg gacgcccagc ggcacgtcca ccccggcgcc aaacgtccag agg
//
LOCUS       DASRZS010000652         7501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5748267, whole genome shotgun sequence.
ACCESSION   DASRZS010000652 DASRZS010000000
VERSION     DASRZS010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7501
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5748267"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VFH83_15880"
     CDS             complement(<1..987)
                     /locus_tag="VFH83_15880"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6487908.1"
                     /translation="MGISIGLVGLGAFGSEFAPLFKSHPLVDRIALCDREPERIARFA
                     RNPSFAAKFKPSDAYSSLEEICKARLDALVLITQPWLHAPQAIQAMESGKHVYSAVPV
                     ICLPDAEEVLQWCDRLVRTAVRTGMHYMLGETTYYRAPTMYCRRQAARGAFGDFVYAE
                     GEYFHPHDWKLADLREVKRSRLASAAGQEWVRIQKGYLDRGIRDTPMMYPTHSTSGPL
                     SVMKTHAVKASAFGYRTRSKDPYFDDHEFTNVTAVYELANGAHMRICEYRELVYPYGD
                     LFDMETFRVFGTQGSFRENRWGDRESWNPLSMEEMRDPLPPEVAAEFQKENGE"
     gene            1173..2660
                     /locus_tag="VFH83_15885"
     CDS             1173..2660
                     /locus_tag="VFH83_15885"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET6487909.1"
                     /translation="MPTEGPLRDAADPLVLGIDLGTTNVKALLVDGKGAVISQGSAPV
                     SVRYTEDGGAEQDIEEIWQATRTAMRDAARDLPEAAGRSSERVRAIGVSSQGGAIQIL
                     DSSDRAVGPIIGWQDARGKPWDRALTSRMGQEWLVQRTGASHSAGAIGQILRLRDSGV
                     FTSRSRLAFAGDIVVGRLCGRRAHDATSLSEPCLLNPSTGDADDALLEVLGLDRSRLP
                     PLLAADSVAGTLLPEVAGSLGLAAGIPVGPAVHDQYAAALGSGTVRAGDTMLGSGTAW
                     VLVAVADHIAPPIGGAALVGRHPVAGLYGQMLSMENGGSCISWATRTLGLGSPGVHEV
                     DRILRSVPDGCAGLRFRPLLQQIASLPPGMTGRIDGLRLEHTAAHVVRAVVEGLACEL
                     GRFLAMMRDAGVRVERLVLCGKAASSAVTPQIISDTIGLPVDCVSAAETSALGAAVLG
                     RALLERGRGLQDLAYALRPSLTRVQPGPGSQGARGRLDAYLEDFR"
     gene            2672..4102
                     /locus_tag="VFH83_15890"
     CDS             2672..4102
                     /locus_tag="VFH83_15890"
                     /inference="COORDINATES: protein motif:HMM:NF014649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487910.1"
                     /translation="MKPFTVGLLPLYIKLYDDLDKDGAWRGRMETFPGAVAHALEKRG
                     VQVERAPICRLAREFAAAVKSLETRSVDALVTLHLAYSPSLESAAVLAKTRLPLIVLD
                     ATPTWSYSPRQDPAELMFNHGIHGVQDMCNLLIRNGKPFLIEAGHWERSDVLDRVAAW
                     LPAARMASTMRRAKVGLIGTAFKGMGDFQVTPTALASTIGASVKSLEPEKFKRLLASV
                     NGDAVGAEMKADRERFRDAGVSAEAHERSVKMGLAIFERSEKMGLAIRRWIEQEGLTA
                     LSFNFQSMVEKDGYPTAPFLEMSKAMERGIGYAGEGDTLTATLVGGLMASFQDTSFTE
                     MFCPDWENNTVFLSHMGEANLRLLDGPPQLREMKYQFSKTRNPALATGCFKPGEVTLV
                     NLAPLGGGRYRLIVAPVEMVPVTGPDRMEESIRGWFRPPVSVADFLAGYSRLGGTHHL
                     AVSYTRDLASLEGLAGMMGWEHARLG"
     gene            complement(4109..5152)
                     /locus_tag="VFH83_15895"
     CDS             complement(4109..5152)
                     /locus_tag="VFH83_15895"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6487911.1"
                     /translation="MAAPFQLNDVDRRVYDRSLRDFLPDAFIDIHTHVWRSQDTAPRD
                     PSSYSRVVSWPSRVAADQPVEDLVSSYQLMFPGKSVSALIFSLVYGGDDMDRMNQYVV
                     EASRRSGFPSLLFAHPTWTASTLEDRIRAGGHKGIKVYLNLAASYIPEREIRIFDFLP
                     PHQLDVLARNRWVVMLHIPRDGRLRDPVNLEQMLEIERSWPGARVIIAHVGRAYCDED
                     LGDAFQKLGRTRDMVFDTSANTNDHVFEAALRAVGPERVLFGSDMPITRMRMRRICEG
                     GRYVNVVPRGLYGDVSGDSHMREADPPQSEELTLFLYEEILAIRRAAERTGLSRADIE
                     NVFNRNAKRVLAV"
     gene            complement(5162..6652)
                     /locus_tag="VFH83_15900"
     CDS             complement(5162..6652)
                     /locus_tag="VFH83_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020618977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6487912.1"
                     /translation="MEAVCAARAFAEGCDRKRLSTVAHEKVDFYPAAFQRRVEELLPL
                     VGSKVCDGLPGSAAGAGTRRFREASKTGPAPLTGLGLFRIGEDGRLYFTSKAEHYHLS
                     VGHSFPGYRLLENARALGIPNCTHNNTRGHITRLLEEELVAAANGLRRQESERLRAVL
                     DATDPRILNRVINLETGSLVAEAAVKMALTRFYRFEPGGSDPKYSGRTPVFLVIGDLE
                     GGTGANYHGTTIATQLMRGLWPELAAHLEQSQTLVVRPVKINDNADFSAVMKQWDQGR
                     YKVAAFFHEIVLMNYGAVRLEESFLQSAHELCRERDVLTVVDEIQSCVWSPELFLFRE
                     YGLSPDIVAVGKGFPGGEYPAARVLLNGSADALQQFGALVTNGQEELASLAYLVTIEY
                     ARAHAAVTREVGDYYESELRSLARRYPRLIDKIEGRRHLASLFFHQMDGVKRFVSLLN
                     EGGIDISVQSYKPSFPPSCLTKLPLTVNARSVDFLVGKMDSVLRKL"
     gene            6845..>7501
                     /locus_tag="VFH83_15905"
     CDS             6845..>7501
                     /locus_tag="VFH83_15905"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET6487913.1"
                     /translation="MRKRELRSARLIEMLQLNRRLDVRTVVESFDISEATVRRMFTQL
                     ENEGKVIRTHGGVRLAPHLGSDYSYFLSASHRNREKTLIGRAAAEIVGSRDRLFLDSG
                     TTVLKLAEALSLRLQSGAVKGIVVLTSSLVLVEALARWCTVILVGGEARLERRDVCGP
                     LSEENLRRYRVNRAFFGADAIHLKSGFMTTDERTAKMDEIALRNSSHSYVLADSEKFT
                     L"
BASE COUNT         1201 a         2465 c         2581 g         1254 t
ORIGIN      
        1 ctcgccgttc tccttctgga actccgccgc gacctctgga ggcaggggat cccgcatctc
       61 ctccatggac agcgggttcc agctctcacg gtccccccaa cggttctcgc gaaagcttcc
      121 ctgggtcccg aagacgcgga aggtctccat gtcgaagaga tcgccatacg ggtaaaccag
      181 ctcgcggtac tcgcagatcc tcatgtgggc gccgttggcc agctcgtaca ccgcggtgac
      241 gttggtgaac tcgtggtcgt cgaagtacgg gtccttgctc cgggtccgat atccgaaggc
      301 gcttgccttc acggcgtggg tcttcatgac cgagagcggg ccagaggtgg aatgggtggg
      361 atacatcatg ggagtgtcgc ggatgccccg gtccaggtat cccttctgga tccggaccca
      421 ctcctgcccg gccgcgctcg ccagccgtga acgcttcacc tctctcaggt ccgccagctt
      481 ccagtcgtgc gggtggaagt actccccctc cgcgtagacg aagtcaccga acgctccccg
      541 cgcggcctgc cgccggcagt acatggtcgg cgctcgatag tacgtagtct cccccagcat
      601 gtagtgcatg cccgtccgaa cggcggttcg gaccagacga tcgcaccact ggaggacctc
      661 ctccgcgtcc ggcagacaga tcacgggcac cgcggagtac acgtgcttcc cgctctccat
      721 ggcctggata gcctgcggcg cgtggagcca gggctgggtg atgagcacca gcgcgtccag
      781 ccgcgccttg cagatctcct ccaggctgga gtaggcatcg gatggcttga acttggcggc
      841 gaaagagggg ttacgcgcga agcgcgcgat gcgctccggc tcccgatcgc acagcgcgat
      901 ccgatctacc aggggatggc tcttgaacaa cggcgcaaac tccgagccga aggctccaag
      961 ccctaccagg ccgatgctga tgcccatccg gagtgccccc tcggtggaat gctaccgggg
     1021 ccacgacgga tccgcaaggg gggagtgatg ccggccggct cgccgccgcc gtcgcggcct
     1081 ccggcgttga caggtccatc cccgcgtgtt tcgatcatca gagccgcgcg ggatggtgaa
     1141 gcgcatcagg cgtgcggcat caaggagaga ccgtgccgac ggaaggaccc ttgcgggacg
     1201 ctgcggatcc cctggtgctg gggatcgacc tgggaaccac caacgtcaag gcgttgctcg
     1261 tcgacgggaa gggcgcggtg atctcccagg gctcggcacc ggtttccgtc cggtacaccg
     1321 aggacggcgg cgcggagcag gacatcgagg agatctggca ggccacgcgg acggccatgc
     1381 gcgacgcggc ccgcgacctc cccgaggcgg ccggtcggag ctccgagcgc gttcgcgcca
     1441 tcggcgtgtc gagccagggc ggcgccatcc agatcctcga ttcgtcggac cgcgccgtgg
     1501 ggccgatcat cggctggcag gacgcccgcg gcaagccgtg ggaccgcgcg ctgacctcgc
     1561 ggatgggcca ggagtggctc gtccagcgca cgggcgcgtc ccacagcgcc ggcgccatcg
     1621 gccagatcct ccggcttcgg gactccgggg tcttcacgtc ccggtcccgg ctcgctttcg
     1681 cgggggacat cgtcgtcggt cggctctgcg gtcggcgagc ccacgatgcc acgagcctct
     1741 ccgagccgtg cctcctcaat ccttctacgg gcgacgccga tgacgcgctc ctggaggtgc
     1801 tcgggctgga tcgatcccgt ctgccgccgc tccttgcggc ggactcggtc gcgggcacgc
     1861 tgctgccgga ggtggcgggc tccctggggc tggccgccgg cattcctgtg ggaccggccg
     1921 tgcacgacca gtacgcggcc gccctgggga gcgggaccgt gcgcgccggc gacaccatgc
     1981 tgggctccgg caccgcctgg gtgctcgtgg ccgtggcgga tcacatcgcc ccgccgattg
     2041 gcggcgctgc cctggtggga cggcatccgg tcgccgggct ctacggccag atgctgtcca
     2101 tggagaacgg cggctcgtgc atctcgtggg ctacgcggac cctgggtctc ggatcccccg
     2161 gcgtccatga ggtcgaccgg atcctgcgct ccgttcccga cggctgcgcc gggcttcgct
     2221 ttcggccgct cctgcagcag atcgcgagcc ttccgcccgg gatgaccgga cggatcgacg
     2281 gcctgcggct ggagcacacg gcggcgcacg tcgtccgcgc ggtcgtggaa ggcctggcct
     2341 gcgagctggg tcggttcctg gcgatgatga gagacgccgg cgtccgcgtg gagcggctgg
     2401 tgctctgcgg caaggcggcg tcgagcgcgg taacgcccca gatcatctcc gataccatcg
     2461 gccttccggt ggactgcgtg tccgcggcag agacgagcgc gctcggcgcc gccgtgctgg
     2521 gacgcgcgct cctggagcgg ggcagggggc tgcaggacct ggcgtacgcc ctgcggccga
     2581 gcctcacgcg cgtgcagccc gggcccggct cgcagggcgc gcggggccgg ctggatgcgt
     2641 atctggaaga ctttcgctga aggagatgtg gatgaaaccg ttcacggttg ggctcttgcc
     2701 gctgtacatc aagctctacg acgatctgga caaggatgga gcctggcgcg gccgcatgga
     2761 gacgttccct ggggcggtgg cccacgccct ggagaagcgg ggcgtccagg tggaacgcgc
     2821 gccgatctgc cgtctggcaa gggagttcgc cgccgcggtc aagagcctgg agacccggtc
     2881 cgtggacgcg ctggtgacgc tgcatctggc atattcccct tcgctggagt ccgcggccgt
     2941 cctggccaaa acccggcttc cgttgatcgt gctggatgcc acgcccacct ggtcctacag
     3001 ccctcggcag gatccggcgg agctgatgtt caaccatggg atccacggcg tccaggacat
     3061 gtgcaacctg ctcatccgca acggaaagcc ctttctcatc gaggcgggtc actgggaacg
     3121 ctcagacgtg ctggaccggg tggcggcctg gctgccggcc gcgcggatgg catccacgat
     3181 gagaagggcc aaggtcggcc tcatcggcac cgcgttcaaa ggcatggggg atttccaggt
     3241 gacgccgacg gcgctggcct ccacgatcgg ggcctccgtc aaatccctgg aaccggagaa
     3301 gttcaagcgc ctcctcgctt cggtgaacgg ggacgcggta ggcgccgaga tgaaggcgga
     3361 ccgggaacgc tttcgggacg cgggagtcag cgcggaggct cacgagcggt ccgtgaagat
     3421 ggggctggcc atcttcgagc ggtccgagaa gatggggctg gccatccgga ggtggatcga
     3481 gcaggagggg ctcacggcgc tgtccttcaa cttccagagc atggtggaga aggatggcta
     3541 ccccacggcc ccgttcctcg agatgagcaa agccatggag cgcggcatcg ggtacgccgg
     3601 ggagggggat accctgaccg ccaccctggt cggcggattg atggcctcgt tccaggacac
     3661 cagtttcacg gagatgttct gcccggactg ggagaacaac accgttttcc tcagccacat
     3721 gggggaggcg aaccttcggc tcctggatgg cccgccccag cttcgcgaga tgaagtacca
     3781 gttctccaag acccgtaacc cggcgttggc caccggctgc ttcaaacccg gcgaggtgac
     3841 gctcgtgaac ctggcgcccc tgggcggagg ccgctaccgg ctcatcgttg cgccggtcga
     3901 gatggtgccc gtgacggggc cggaccgcat ggaggagtcc atccgcgggt ggttccgacc
     3961 accggtgtcc gtggcggact tcctggccgg ctacagccgg ctgggcggga cgcaccacct
     4021 ggccgtgtcc tacacgcggg atctcgcgtc cctggaaggc ttggctggga tgatgggatg
     4081 ggagcacgcg cgcctggggt agcggtgttt acaccgccaa cacgcgcttc gcgtttcggt
     4141 tgaatacgtt ctcgatgtcg gcgcggctca ggccagtgcg ctccgccgcc ctgcggatgg
     4201 ccaggatctc ctcgtagagg aacagcgtca gctcctccga ctgcggaggg tcggcctccc
     4261 gcatgtggct gtccccgctc acgtccccgt agaggccgcg gggcacgacg ttcacgtagc
     4321 ggcctccctc acagatgcgg cgcatccgca tgcgggtgat gggcatgtcg cttccgaaga
     4381 ggacccgctc gggacccacg gctcggagcg ccgcctcgaa cacgtggtcg ttcgtgttgg
     4441 ccgacgtgtc gaaaaccatg tcacgcgtcc tgcccagctt ctggaacgcg tctcccaggt
     4501 cctcgtcgca gtaggcgcgg cccacgtggg cgatgatcac ccgcgcgccc ggccagcttc
     4561 gctcgatctc cagcatctgc tcgaggttga ccggatcgcg aagccgcccg tctcttggga
     4621 tgtggagcat cacgacccag cggttgcggg ccaggacatc gagttgatgc ggcgggagga
     4681 aatcgaagat gcggatctcc cgctcgggga tgtaggatgc cgccaggttc aggtagacct
     4741 tgatcccctt gtgccctccg gcgcggatcc ggtcctccag cgtcgacgcc gtccaggtgg
     4801 ggtgcgcgaa gaggagcgac gggaacccgg atcgccggga cgcctcgacc acgtactgat
     4861 tcatccggtc catgtcgtcg ccgccgtaga cgagggagaa gatcagggcg gacacggatt
     4921 tgcctgggaa catgagctgg taggaggaca ccaggtcctc gacgggctgg tccgccgcaa
     4981 cccgcgacgg ccagctcacc acgcgagagt aggaggaagg atcgcgcggg gccgtgtcct
     5041 gggaacgcca gacgtgcgtg tgaatatcga tgaacgcgtc cgggagaaaa tcccgcaggg
     5101 atcgatcgta gacccggcgg tctacgtcgt tgagctggaa cggtgcggcc atccgcgtct
     5161 cctacagctt tctgaggacg gagtccatct tgcctaccag gaagtccacc gagcgagcgt
     5221 tcacggtgag gggtagcttg gtcaggcagg acggcgggaa gctgggcttg tagctctgca
     5281 cgctgatatc gatgcctccc tcgttcagga gagaaacgaa ccgcttgacc ccgtccatct
     5341 ggtggaagaa cagggacgcc aggtggcgcc gcccctcgat cttgtcgatg aggcggggat
     5401 accggcgggc cagggaccgc agctccgact catagtagtc cccgacttcc cgggtcacgg
     5461 cggcgtgcgc gcgtgcgtac tcgatggtga ccaggtatgc cagggacgca agctcctcct
     5521 ggccgttggt gaccagcgcg ccgaactgct gcagggcatc cgcggatccg ttcaggagca
     5581 cgcgcgcggc cgggtattcc ccgccgggga atcccttgcc cacggccacg atatccggcg
     5641 agaggccgta ctcccggaac aggaagagct cgggagacca gacgcaggac tggatctcgt
     5701 ccaccacggt cagcacgtcc cgctcgcggc acagctcgtg agcggattgc aggaagctct
     5761 cttccaaccg gacggcgccg tagttcatga gcacgatctc gtggaagaac gcggccacct
     5821 tgtaccgccc ttggtcccat tgcttcatca cggcggagaa gtcggcgttg tcgttgatct
     5881 tcacgggacg gaccaccagg gtctggctct gctccaggtg ggcggccagc tcgggccaga
     5941 gcccacgcat gagctgggtg gcgatcgtcg tcccatggta gtttgcgccg gtgccgccct
     6001 cgaggtcccc gatcacgagg aacaccggag tcctgccgga gtacttgggg tccgagccgc
     6061 ccggctcgaa ccggtagaac cgcgtgagcg ccatcttcac cgcggcctcc gccaccaggc
     6121 tgcccgtctc caggttgatg actcggttga ggatccgtgg gtccgtggcg tccagcacag
     6181 cgcggaggcg ttcgctctcc tggcggcgca gcccgttggc cgccgccacc aactcctcct
     6241 ccaggagccg ggtgatgtgc ccgcgggtgt tgttgtgcgt gcagttgggg atgccaagcg
     6301 cgcgcgcgtt ctccaggagc cggtagccgg ggaagctgtg acccacggag aggtggtagt
     6361 gctcggcctt ggatgtgaag tagaggcggc cgtcctctcc gatgcgaaag agcccgagcc
     6421 ccgtcagcgg ggcgggcccc gtcttgctgg cctcccgaaa ccgccgcgtg ccggccccag
     6481 cggcggagcc ggggagccca tcgcacacct tgctgcctac gagcggcagc agctcctcca
     6541 cgcggcgctg gaacgccgcc ggatagaagt cgaccttctc atgggcgacc gtcgagagcc
     6601 gttttcggtc gcaaccctcc gcgaaagccc tggcggcgca gaccgcctcc acatactctt
     6661 ccccgaccag ctctgccagg gaacggcaaa cgtcattgtc tgacgtggta cgtctcctta
     6721 ttgatcgttt gggccaaaac agggcgagta tagcaacttt cgtctatccg tcaagggaag
     6781 aatgatcgga tcgatcagaa tggtccttga cgcgccgaaa cgcttccgaa atactgcgct
     6841 ggcgatgagg aaacgcgagc tgcgatccgc acgtctcatc gagatgctgc aactgaaccg
     6901 gcgcctggac gtacggacgg tggtcgagtc cttcgacatc tccgaggcca cggtccgcag
     6961 gatgttcacc cagttggaga acgagggaaa ggtcatccgc acccatgggg gcgtgcggct
     7021 ggcgcctcac ctgggctcgg actactccta cttcctctcc gcaagccaca ggaaccgcga
     7081 gaagacgctg atcggccgag cggcggcgga gatcgttggc agccgggaca ggctcttcct
     7141 ggactccggc accaccgtcc tcaaactggc ggaagcgctc tcgctgcggc tccagtccgg
     7201 ggccgtcaag gggatcgtcg ttctcacctc ttccctggtg ctggtggagg ccctggcgcg
     7261 ctggtgcacc gtcatcctgg tgggggggga agcccggctg gagcgcaggg acgtgtgcgg
     7321 gccgctctcc gaggaaaacc ttcgtcgcta ccgggtcaac cgggccttct tcggcgcgga
     7381 tgccatccac ctcaagagcg ggttcatgac cacggacgag cggaccgcca agatggacga
     7441 gatcgcgctg cgcaactcct ctcactccta cgtgcttgcg gactccgaga agttcaccct
     7501 g
//
LOCUS       DASRZS010000653         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3836358, whole genome shotgun sequence.
ACCESSION   DASRZS010000653 DASRZS010000000
VERSION     DASRZS010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3836358"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VFH83_15910"
     CDS             complement(<1..636)
                     /locus_tag="VFH83_15910"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HET6487914.1"
                     /translation="MKRVILLAALVAAFATMGTSVWANGSMEKKPIVWVWLPNDSPPE
                     SAEFRAAMDKIVSDATGRPVQDKLTTDYNIAIQAMVSDNAAIALFGPYQYVKCSALSK
                     AVVPIVTNTGDSGTLSDAVYYSRIVVKTENASQYMKNGQYSLDAIAGKIFSFVSNSST
                     SGFLFPSSGVAAYFSKMSQWSTLKQSDLMEGGQGKLFNQVVFGGSHQGSLAN"
     gene            842..1615
                     /gene="phnC"
                     /locus_tag="VFH83_15915"
     CDS             842..1615
                     /gene="phnC"
                     /locus_tag="VFH83_15915"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007086604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HET6487915.1"
                     /translation="MNGESAVLEVRNLSKSYGEVQALADVNLSVGRGELVALIGPSGA
                     GKSTLLRCINRMIDATTGEVVFENVDVQRLARADLRRVRTKIGMIFQHYNLVYRLTVI
                     ENVLHGRLGYLRGVSGIIGRYSELDKARAVEIIRRVGLLEQIYKRCDALSGGQMQRVG
                     IARALIQDPQIILCDEPIASLDPSTAKVIMDYLRTISSELGIAVLVSLHQVDVALKYA
                     DRILGVNGGRIVFDGIPAALTREMVYEIYDKDEGELVLD"
     gene            1602..>2318
                     /locus_tag="VFH83_15920"
     CDS             1602..>2318
                     /locus_tag="VFH83_15920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6487916.1"
                     /translation="MSSTERTAPDGTASSLEVFKARRRQMTQVLLVIAAIVAVSSIIT
                     QFSYRQAAFSVPKAFGWLVTNIIPDRPALAKLPTIVAKLLQTIVVAVMATVFATAAAF
                     LLAILGSTATRPHPLFSLAARAIGSVFRNIPVVAWAMVLLLSFGQSLLTGLLALFFAT
                     FGFLVRSFLETIDETSRSSVEALHAAGASWPQTVFQAVLPSALPQVLSWMLYMVETNI
                     RDATLVGILTGTGIGFTFDLY"
BASE COUNT          397 a          750 c          726 g          445 t
ORIGIN      
        1 gttcgcgagc gacccctggt gtgaaccgcc gaataccacc tggttgaaga gcttgccctg
       61 cccgccctcc atgagatcgg actgcttcag cgtgctccac tggctcatct tggagaagta
      121 cgccgccacg cccgaggacg ggaacaggaa gcccgaggtc gagctgttgg aaacaaaaga
      181 gaagatcttg ccggcgatgg cgtccaggga gtactggccg ttcttcatgt actgggaggc
      241 gttctccgtc ttcacgacga tgcggctgta gtacaccgcg tcggagagcg ttcccgagtc
      301 tcccgtgttc gtgacgatgg gaacgacggc cttgctgagc gcgctgcact tcacgtactg
      361 gtaggggccg aacagcgcga ttgccgcgtt atcggaaacc atggcctgga tcgcgatgtt
      421 gtagtccgtg gtgagcttgt cctgcacggg cctgcccgtg gcgtcagaca cgatcttgtc
      481 catcgccgcg cggaactccg ccgattccgg cggtgagtcg ttgggcagcc acacccacac
      541 gatcggcttc ttctccatgg agccgttggc ccataccgag gtccccatgg tcgcgaaggc
      601 ggctaccaga gccgccaaca ggatcacccg cttcatgaca ataccccctt gcatcgattc
      661 gcgccggcac cagccgcgac gcgttgccat tgacttgggg cggcggcgtt aggattgcgt
      721 gagcgcagcg cgaattccgt gttacggcag gggtcttcat tcgctcaata ctagccaact
      781 gctaacgaat tcccaacggt tcccgctgta gcctcaccat cggggtgaca ggaggctgca
      841 agtgaacggg gaaagtgcgg tcctggaggt tcgaaacctt tccaagagct acggcgaggt
      901 acaggcgctg gcggacgtga atctctccgt gggcagggga gagctggtgg cgctcatcgg
      961 tccgtcagga gccggcaagt ccactttgct tcgctgcatc aaccgcatga tcgacgcgac
     1021 caccggcgag gtggtcttcg agaatgtgga cgtccagcgc ctggcgcggg cggaccttcg
     1081 cagggtccgc accaagatcg gcatgatctt ccagcactac aacctggtgt accgcctgac
     1141 cgtgatcgag aacgtgctgc acgggcgcct cgggtacctg cgcggagtga gcggcatcat
     1201 cggccgctac agcgagctgg acaaggcacg cgcggtggag atcattcgtc gcgtggggct
     1261 cctggagcag atctacaagc ggtgcgacgc cttgagcgga gggcagatgc agcgcgtggg
     1321 aatcgccagg gccctcatcc aggaccccca gatcattctg tgcgacgagc ccatcgcctc
     1381 cctggacccc agcaccgcca aggtcatcat ggactatctc cgcacgatct cgagcgagct
     1441 tggcatcgcg gtgctggtga gcctgcacca ggtggatgtt gctctgaagt acgcggaccg
     1501 catcctcggc gtgaacgggg ggcggatcgt gttcgacgga atcccggcgg ccctcacgcg
     1561 ggagatggtg tacgagatct acgacaagga cgagggcgag cttgtcctcg actgaacgga
     1621 cggctcccga cggcacggct tcctccctgg aggtcttcaa ggcccgacga cgacagatga
     1681 cccaggtcct cctggtcatc gccgccatcg tggccgtctc ctccattatc acgcagttca
     1741 gctaccggca ggcagcgttt tccgtcccca aagcgttcgg ctggctggtg accaacatca
     1801 tcccggaccg cccggccctt gcgaagcttc ccacgatcgt tgccaagctc ctgcagacga
     1861 tcgtcgtggc agtcatggcc accgtgttcg ccaccgcggc cgcgttcctt cttgccatcc
     1921 tgggctcgac cgctacgcga ccccaccccc tgttttccct ggcggcacgc gccatcgggt
     1981 cggtattccg caacatcccc gtcgtcgcgt gggccatggt gctgctgctg tccttcggcc
     2041 agagccttct cacggggctt ctggcgctgt tctttgccac gttcggattc ctcgttcgtt
     2101 ccttcctgga aaccatcgac gagacctccc gcagcagcgt ggaggccctg cacgccgccg
     2161 gggcgagctg gccgcagacg gtgttccagg cggtgctccc ctccgcgctc ccccaggtgc
     2221 tgagctggat gctgtacatg gtggagacga atatccgcga cgcgacgctc gtggggatcc
     2281 tcaccggcac cggcatcggg ttcacgttcg acctgtat
//
LOCUS       DASRZS010000654         6158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4984285, whole genome shotgun sequence.
ACCESSION   DASRZS010000654 DASRZS010000000
VERSION     DASRZS010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4984285"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2569)
                     /locus_tag="VFH83_15925"
     CDS             complement(<1..2569)
                     /locus_tag="VFH83_15925"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET6487917.1"
                     /translation="MTASVLLDRNGIRIDGRYRVLLCSSVHYFRIPRELWKDRLQRVK
                     DAGYTCVDLYFPWNFHERAPGRWDFSAERDAAAFLDLVTEVGLYAVARPGPYICSEWD
                     GGAIPAWILASGMRIREDSEPFLTEVRGWYERIVPLIAAREVGRGGSVILFQVDNELD
                     FFDCPDPAAYIGSLASLARKLGITVPVFGCAGQGSAERATGFAPGVEVTFNLYPSAAD
                     PHFDQGCRLAAAALRSVDRPLLVTETNREHAVLRRELANGTKLLGAYNQVAGSNFGFT
                     ASINNLGKPSPLSFIASDYNFRSMVDAAGEYGPEAAEGRLLGALIETLGESLACATVE
                     DEPPVAVTTDFPTNAGAPAVLRLRDGGYLVCVPNLSETCGTATVRGPGFELKVAVGPM
                     RSPLIPLDVPIDRSGVSGRIVGSSAEILAIEEGRLVLYADGDAEVVLALGADAPVRVR
                     SASAPTILTHGGKSLQVLFLTREQAAAGWPGHPWRPAGGRRPSSVTLSAARKAPFEPA
                     WIPADATRDGAITMEARGLYRGFALYDLGEDAPRSLLLRGVADVVTAYDGGRRLDTRI
                     SAGQWQRYQGTTGAWRFWTESWGHSNFDDSRLPSMRLCSSKGIESVFEVVREESLAGQ
                     WRFRLMDQWLPKRLEYPDHPFDAALDPNQWNSTRSPLLALYRTDVTLAEGSQELALHL
                     DGGRAEVALYVGGDLVGVMNPNDPWIGVPSSRISGGHARLELLCRKRDWAEPVGAPTL
                     LHLRRLDPKVLSFGEEQLAGLRAATGARAELPLTLERDATCMVDVDLAGLEHRCGYAV
                     FDGRDVKLTAVFDGRVVGRIFCHAENRPFVTGGDGMRCYLPGPWYDARDNHLVLLVEP
                     "
     gene            complement(2684..4219)
                     /locus_tag="VFH83_15930"
     CDS             complement(2684..4219)
                     /locus_tag="VFH83_15930"
                     /inference="COORDINATES: protein motif:HMM:NF023435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6487918.1"
                     /translation="MARFPGSKVLIFAAAFVIGFALSASVATAQASTSNFVTVDFQVM
                     GDAPANGEADVVLAKINDYLKTKINAAMRFRWTSWTDWQTQYNLLVATGQGLDLVTAA
                     SDWLDLWPNAAKGAWMPLDSLLPKYAPKTWAEIPKTDWDQSRFQGKIIAFPENSYTQY
                     VNHGLFYRGDWARKAGLSKITSWNDLTRYLGYIKQNMPDVIPWDNANNWEHAEGWLIS
                     HTEAVPVDAVAGRLLYFKSFRTDPYTVYSPFFDKTFDDFAALMKQWADAGYWKADVLN
                     NKDDTRQFLRDGKTGMDEHHVQTYAAGSTSLRNLMDKDQPGSDLQMFGFFEPTKNLIK
                     MPILHGATTISAGSKNPERATMLYELIRQDQTLYMLLNYGILNRNYYLNEKGQLYVPP
                     TFDLTKFQFYSDFWGGRVDKFEPKNASVWSGHDAYIKYLDGFAKPYLYSNFFFDQTPV
                     STQVAAISQVANAMIPAIALGMAGDPKQAVADFRSQLKTAGIDQVIAEVQKQVNAWRA
                     SNK"
     gene            complement(4482..5543)
                     /locus_tag="VFH83_15935"
     CDS             complement(4482..5543)
                     /locus_tag="VFH83_15935"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6487919.1"
                     /translation="MDSRAKLTIYGIAREAGVSPSTVSRVMTGSARVREDKRKKVEAV
                     IRRRHFRPSAFARALSRRRTGVFGFILPDISHPYYGLVFLGAENRAAELGFSLILGNT
                     LNDNADHVTHMETQLLDVMMDRGADGIILAGGRVQETRPIEAHLEQIRRIASRVPVVT
                     VSGRMQRAPCWSVEQDEERAVEIAVNYLVTLGHRSIGFLGGVAGIEPSDTRRRSLRKN
                     LDAHGLEWREEWCRYGGFTVEDGRGGMEAFLQIRNRPTAIIAFNDLNAVGAIYTARKN
                     GLRVPEDLSIVGIDDIPLVEYVVPRITSVDLCPREQGSQAVDVMAAILRGESPRRRTV
                     LEPRLVIRDSCRMLTPHSA"
     gene            5693..>6158
                     /locus_tag="VFH83_15940"
     CDS             5693..>6158
                     /locus_tag="VFH83_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6487920.1"
                     /translation="MATRSSTPTVGQALKRPSRNSTWARLWNTRGLLLMCLPAILFFL
                     IFYYLPMPGAYLAFVDFNYRAGIFKSRFIWFKNFEFLLANGQLRSLTLNTILYNVGFI
                     VTGSIAQILIALLLNELRGKAFRKMTQTVMFLPYFISYVLVGLFTYSLLSYDS"
BASE COUNT          995 a         2054 c         2012 g         1097 t
ORIGIN      
        1 tgggctcgac caggagcacc aggtggttgt cccgggcgtc gtaccacggg ccgggcaggt
       61 agcagcgcat cccgtccccc ccggttacga aaggtcgatt ctcggcgtgg cagaagatgc
      121 ggccgaccac ccgtccgtcg aacacggccg tgagcttcac gtcacggccg tcgaacaccg
      181 cgtagccgca ccgatgctcc agcccagcca ggtcgacatc caccatgcac gtggcgtcgc
      241 gttccaaggt gagggggagc tctgcgcgcg ctcccgtggc cgctcgcaag cctgcaagct
      301 gctcctcccc aaagctcagc accttggggt ccaatcgccg aaggtggagg agggtagggg
      361 cgcccacggg ctcggcccag tcacgctttc ggcagaggag ctccagacgc gcgtgcccgc
      421 cggagatcct gctggacggg actccaatcc acgggtcgtt ggggttcatc acgccgacca
      481 gatcgccgcc aacatacagc gccacctccg cccggccgcc gtccaggtgg agcgccagct
      541 cctggcttcc ctcggccagc gtcacgtcgg tgcggtagag cgcgagcagc gggctgcgcg
      601 tggagttcca ctggttggga tcgagggccg catcgaacgg atggtcggga tactcaagcc
      661 gcttgggcag ccactggtcc attagtcgga agcgccactg ccctgccaga gactcctccc
      721 gcaccacttc gaacacggac tcgatcccct tgctgctgca caggcgcatg gacggcagcc
      781 gggaatcatc gaagttggag tgcccccagg actcggtcca gaaccgccac gcgccggtgg
      841 tgccctggta gcgctgccac tgccccgcgg agatccgggt gtccagtctc cgccctccgt
      901 cgtaagccgt gaccacgtcg gccacgcccc gaagcagcag ggaccgcggg gcgtcctccc
      961 ccaggtcata gagagcgaag ccgcggtaga ggccgcgcgc ctccatcgtg atggcgccgt
     1021 ccctcgttgc gtccgccggt atccatgcgg gctcgaacgg ggctttccgg gcggctgaga
     1081 gcgtcaccga ggatggccgg cgcccacccg cggggcgcca cggatggccg ggccagcccg
     1141 cggcggcctg ctcccgggtc aggaagagga cctggaggct cttccctccg tgggtcagaa
     1201 tcgtgggggc cgacgcggac cggacgcgga ccggcgcgtc cgcgccgagc gcaagcacca
     1261 cctccgcgtc cccgtccgcg tagagcacca gcctgccttc ttcgatggca aggatctccg
     1321 cggaggagcc cacgatccgg ccgctcacgc cggaccggtc gatcggcacg tccaggggaa
     1381 tcagcggcga gcgcatcggt ccgacggcta ccttcagctc gaagcccggc cctctcaccg
     1441 ttgccgtacc gcaggtctcc gagaggttgg ggacgcacac gaggtatccc ccgtccctca
     1501 gccgaaggac cgcgggcgcg cccgcgttgg tcgggaagtc cgtggtcacc gcaacgggcg
     1561 gctcgtcctc cacggtggcg caggccagac tctctcccag ggtctcgatc agcgccccca
     1621 ggagacgccc ctcggcggct tccgggccgt attcccccgc tgcgtcgacc atcgagcgga
     1681 agttgtagtc actggcgatg aaggacagcg gcgacggctt tcccaagttg ttgatgctgg
     1741 ccgtgaaccc gaagttcgat ccggccacct ggttgtacgc gcccaggagt ttcgtgccat
     1801 tggcgagctc ccgccgcagg accgcatgct ctcgattggt ctccgtcacc aggagcggtc
     1861 gatccacgga tcgaagcgcc gctgccgcca gacggcatcc ctggtcgaag tgagggtccg
     1921 ccgccgatgg atacaggttg aaggtgacct ccacgccggg ggcgaagccg gtagcccgct
     1981 ccgcgctgcc ctgtcccgcg cagccgaaga ccgggaccgt gatgcccagc ttccgcgcaa
     2041 gggaggcgag agagccgatg taggccgccg gatccgggca atcgaagaag tccagctcgt
     2101 tgtcgacctg gaagaggatg accgaacccc cgcggcccac ctcccgcgcc gcgatgagag
     2161 gcacgatcct ctcgtaccag cctcgcactt ccgtgagaaa gggctcgcta tcctcgcgga
     2221 tccgcatgcc ggaggcgagg atccacgcgg ggatggcgcc cccgtcccac tcgctgcaga
     2281 tgtaggggcc ggggcgggct accgcgtaca ggccgacttc cgtgaccagg tccagaaagg
     2341 cggccgcatc gcgctccgcg ctgaaatccc agcggcccgg agcgcgctcg tggaagttcc
     2401 acgggaagta gagatccacg caggtatacc cggcgtcctt cacgcgctgg agacggtcct
     2461 tccacagctc ccgcgggatt cggaagtagt gcaccgacga gcagaggagc acccgatacc
     2521 gtccatcgat gcgaatgccg ttgcggtcga gcaggacgct tgcggtcata gttgcctttc
     2581 aagccagaga ggaaacgggc ggggcacctg ccccgcccga catcagattc cgcgaaaggg
     2641 aatggttcgc gccgtggggg gcggcgaacc gaacgaccat cggctacttg ttgctggccc
     2701 tccaggcgtt gacctgcttc tggacctcgg cgatgacctg gtcgatgccg gcggtcttga
     2761 gctggctgcg gaaatccgcg acggcctgct tggggtcccc cgccatgccc agggcgattg
     2821 ccgggatcat cgcattggcc acctgggaaa tggccgccac ctgggtgctc accggggtct
     2881 ggtcgaagaa gaagttgctg tacaggtacg gcttggcgaa gccgtccagg tacttgatgt
     2941 aggcgtcatg gccgctccat acgctggcgt tcttcggctc gaacttgtcc acgcggccgc
     3001 cccagaagtc cgagtagaac tggaacttgg tcaggtcgaa ggtgggcggt acgtacagct
     3061 gccccttctc attcaggtag tagttgcggt tcaggatgcc gtagttcagc agcatgtaga
     3121 gcgtctggtc ctggcggatc agctcataga gcatggtggc ccgctcgggg ttcttgctcc
     3181 ccgcggagat cgtggtggcg ccgtggagga tgggcatctt gatgaggttc ttggtgggct
     3241 cgaagaaccc gaacatctgc aggtcgctcc ccggctggtc cttgtccatc aggttgcgga
     3301 ggctggtgga tcccgcggcg taggtctgga cgtggtgctc gtccataccg gtcttcccat
     3361 cgcgcaggaa ctggcgcgtg tcgtccttgt tgttcaggac gtcggccttc cagtaccccg
     3421 cgtccgccca ctgcttcatg agcgcggcga agtcatcgaa ggtcttgtcg aagaagggtg
     3481 agtagacggt gtacgggtcc gtccggaagg acttgaagta caggagacga ccggcaaccg
     3541 cgtcgaccgg gaccgcctcc gtgtgggaga tgagccatcc ttcagcgtgc tcccagttgt
     3601 tggcgttgtc ccaggggatc acgtccggca tgttctgctt gatgtagccg aggtagcggg
     3661 tgaggtcgtt ccagctcgtg atcttgctga gtccggcctt gcgggcccag tctccccgat
     3721 agaagaggcc gtggttgacg tactgggtgt acgagttctc ggggaaggcg atgatcttgc
     3781 cctggaaccg ggactgatcc cagtcggtct tggggatctc tgcccaggtc ttcggggcgt
     3841 acttgggaag caggctgtcc aggggcatcc acgcgccctt ggccgcgttt ggccacaggt
     3901 ccagccagtc cgatgccgcg gtcaccaggt cgaggccctg accggtggcg accagcaggt
     3961 tgtactgggt ctgccagtcg gtccagctcg tccatcggaa ccgcatggcg gcgttgatct
     4021 tggtcttcag gtagtcgttg atcttggcca ggaccacgtc cgcctctccg ttggccggag
     4081 cgtctcccat gacctggaag tccaccgtga cgaagttgga cgtagaagcc tgtgccgtgg
     4141 cgacgctggc cgatagggcg aacccgatca cgaacgccgc ggcgaagatc agaaccttgg
     4201 atccagggaa tcgtgccatg gacacctcct tgagagtcgt gcgggcacct cgatccggcc
     4261 cggttctggc atcacccggc cgcaaccccg aaaccgcttt cagaaccggg ccgttggtcc
     4321 cctgcgtccc catgagacca cagtccccgg gcgctgtcaa gtgaaatcgg ttccgtccgc
     4381 agtgttcatt ccatgacacc ggtttcagat ttcggcgacg ccgccgacgt tgcggccggc
     4441 ggcgtaacgg taccagccgt ccaagagagt ggagactcgg actatgcgct atgcggagtg
     4501 agcatgcggc aggagtcgcg gatcaccagc ctgggctcca ggaccgtgcg gcgtctgggg
     4561 ctctctccgc gaaggatggc ggccatgacg tccacggcct ggctcccctg ctcccgggga
     4621 cagaggtcca cgctggtgat ccggggcacc acgtactcca ccagggggat gtcgtcgatt
     4681 cccacgatgg agaggtcttc gggtacgcgc agcccgttct ttcgggccgt gtagatggcg
     4741 ccgaccgcgt tgaggtcgtt gaaggcgatg atggccgtgg gccgattgcg gatctggagg
     4801 aacgcctcca tgcccccgcg gccgtcctcc accgtaaagc cgccgtagcg gcaccattcc
     4861 tcccgccact ccagaccgtg agcgtccagg ttcttgcgca acgatctgcg gcgcgtgtcg
     4921 ctgggctcta tccccgccac gcctcccagg aagcctatgg accgatggcc cagcgtcacc
     4981 aggtagttca cggcgatctc taccgcgcgc tcctcgtcct gctccacgga ccagcacggc
     5041 gcgcgctgca tccggccgct cacggtgacc accggtaccc ggctggcgat ccggcggatc
     5101 tgctcgaggt gcgcctcgat ggggcgggtc tcctggaccc gtccgccggc caggatgatg
     5161 ccgtccgcgc cccggtccat catgacgtcc agaagctgcg tctccatgtg ggtgacgtga
     5221 tccgcgttgt cgttcagggt gttgcccagg atgagggaga accccagctc cgccgcgcgg
     5281 ttctccgcgc ccaggaagac cagaccgtag tagggatgcg agatgtccgg caggatgaac
     5341 ccgaacaccc ccgtgcgccg cctgctgagg gcccgcgcga aggcgctggg gcggaagtgc
     5401 cgtcttcgga tgacggcctc caccttcttg cgcttgtcct ctcggacccg ggcgctgccc
     5461 gtcatgacac gggacaccgt ggacggggac acccccgcct cccgcgcaat cccgtagatg
     5521 gtgagctttg cccggctgtc caaggttgtc ctatttcgca ccacagggcg cgccgtgtca
     5581 aggattgcgg tttttcttga cagccaccgc gctctgtggt ctcatgggtg cggttcggct
     5641 cgggaccgcc cgggcctgaa agcgctttcg cgcgcgtggg gaggtaggga ccatggcgac
     5701 ccgcagctcg acaccgaccg tcggtcaggc gctgaagagg ccctcccgca attcgacctg
     5761 ggcccgacta tggaacacgc gcggtctcct cctgatgtgc cttcccgcca tcctgttctt
     5821 cctgatcttc tactaccttc ccatgcccgg ggcctatctg gcgttcgtgg acttcaacta
     5881 ccgcgccggt atcttcaaaa gccggttcat ctggttcaag aacttcgagt tcctgttggc
     5941 caacggtcag cttcgcagcc tcacgctcaa cacgatcctg tacaacgtcg gattcatcgt
     6001 caccggcagc atcgcgcaga tcctgatcgc gctcctgctg aacgagcttc gcggcaaggc
     6061 gtttcgcaag atgacccaga ccgtcatgtt cctgccgtac ttcatctcct atgtcctggt
     6121 aggactcttc acgtacagcc tcctgagcta cgactccg
//
LOCUS       DASRZS010000655         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1154076, whole genome shotgun sequence.
ACCESSION   DASRZS010000655 DASRZS010000000
VERSION     DASRZS010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154076"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..2123
                     /gene="glmS"
                     /locus_tag="VFH83_15945"
     CDS             288..2123
                     /gene="glmS"
                     /locus_tag="VFH83_15945"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET6487921.1"
                     /translation="MCGIVGYIGDKKVVPILIDGLKRLEYRGYDSAGLAVLEQGRMVV
                     YRSVGKIKELESAVWGANLTGTVGLGHTRWATHGRPTEENAHPHTDCSGDLVVIHNGI
                     IENYLALKEKLLAEGHVFRSQTDTEVIAHLIEKYLKEDPDIERATRRALNDLTGAYAV
                     GVLWKGDPGRIVGARCQSPLVVGLGEGDFFFASDIPAILSHTRNVLFLNDGEMVVLTK
                     EGVQVTTLSGETVQRPVETVLWSPILAEKGGYKHFMLKEIYEQPRAVRDTIRGRFSED
                     SATIFVEGMEQLEEQLTSIDKVILIACGTAWHAGLVGKFMIEELARVSVEVDYSSEFR
                     YRDPVIGERTLVLAISQSGETLDTLAGIKEARARGCHTAAICNVLGSSLTREVDGVLY
                     THAGPEIGVAASKTFTAQLAALYLFALYLGQRRGALDATRIRALLADLVRLPQQIEQV
                     LHLDTETERLAHQYHAKTDFLYLGRGVNYPIALEGALKLKEISYIHAEGYPAGEMKHG
                     PIALITEDMPVVILAPQDKVYEKILGNIQEVKARDGIVIAVCSEGDEELSKKADQVLT
                     VPRASAYLTPILLAIPMQLLAYHVAVHKGCDVDQPRNLAKSVTVE"
BASE COUNT          451 a          665 c          716 g          375 t
ORIGIN      
        1 tgttactgcc acatgatgag gcaactgtca cacaagcaac aaaaaggata gcaagcgtag
       61 gggcccctcc ggcttccggt cagaagccta aaccccgctg cgacacgcct cgcgatcccg
      121 ggatgggcgg ggtcctttcg cgacgggcag gctggtccgc gttctgcatc cctgtccgac
      181 gaagaacgga caactggcaa tggaggaccg ttcaagggct tttgttctcg agtggtccgc
      241 tatcctggat ggattctaga gggtaacccc gagcgaaagg acaggccatg tgcgggattg
      301 tcgggtatat cggggacaag aaggttgtgc cgatcctgat cgacgggcta aagcgattgg
      361 aataccgggg ctatgattct gcggggttgg ccgttctgga gcagggtcgg atggtggtgt
      421 accgctcggt cgggaagatc aaggaactgg agagcgccgt ctggggagcc aacctcaccg
      481 gcactgtggg gctcggccac acccgctggg cgacgcatgg ccgcccaaca gaggaaaacg
      541 cccacccgca cacggactgc agcggcgacc tggtcgtcat tcacaacggg atcatcgaga
      601 attatctggc tctcaaggag aagctcctgg ccgaagggca cgtttttcgc tcgcagaccg
      661 ataccgaagt catcgcccac ctgatcgaaa agtatctgaa ggaagacccg gacatcgaac
      721 gggccacgcg ccgggctttg aacgacctca ccggggctta cgccgtcggc gtcctctgga
      781 agggcgatcc tggccgcatc gtgggggccc gctgccagag cccgctggtg gtgggactgg
      841 gtgaagggga cttcttcttc gcctcggaca taccggccat cctcagccac acgcggaatg
      901 tcctcttcct caacgacgga gagatggtcg tgctcacgaa agagggggtg caggtcacga
      961 ccctgagcgg ggagacggtc cagcgcccgg tggagacggt cctgtggagc cccatcctgg
     1021 ccgaaaaggg cggctacaag cacttcatgc tcaaggagat ctacgagcag ccgcgcgccg
     1081 tccgggacac catccggggg cggttttccg aggacagcgc gacgatcttc gtcgaaggca
     1141 tggagcaact cgaggagcaa ctcacctcca tcgacaaggt catcctgatt gcttgcggca
     1201 ccgcctggca tgcggggctg gtgggcaagt tcatgatcga ggagttggcc cgcgtgtcag
     1261 tggaggtgga ttacagctcc gaattccgtt atcgcgatcc ggtgatcggg gagcgcaccc
     1321 tggtcctggc cattagccag tccggcgaga cgctggacac cttggcaggg atcaaggagg
     1381 cgagggcgcg cggctgccat accgcggcca tctgcaacgt gctggggagt tcactcaccc
     1441 gcgaggtgga tggcgtcctc tacacccacg ccggtcccga gatcggtgta gccgccagca
     1501 agaccttcac ggcgcagctt gcggcactgt atctgttcgc gctctacctc ggacagcggc
     1561 gaggtgcctt ggacgcgacg cggatccgcg cgctcctggc ggatctcgtc cgcctgcccc
     1621 agcagatcga gcaggtcctg cacctggaca ccgagacgga gcggctggcc catcagtacc
     1681 acgccaagac ggacttcctc tacctcgggc gcggcgtcaa ctatccgatc gcgctggagg
     1741 gagccctgaa gctcaaggag atctcttaca tccacgccga aggctaccct gcgggcgaga
     1801 tgaagcatgg gcccattgcg ctcatcaccg aagacatgcc ggtggtcatc ctggctccgc
     1861 aggataaagt gtacgaaaag atccttggga acattcagga ggtcaaggcg cgggacggca
     1921 tcgtcatcgc cgtctgctcg gagggcgacg aggagctctc gaagaaggcc gaccaggtct
     1981 taaccgtgcc ccgcgcctcc gcctacctga ctcccatctt gctggccatt ccgatgcaac
     2041 tcctggccta tcacgtcgcg gtccacaaag gctgcgacgt ggaccagccg cgaaacctgg
     2101 ccaagagcgt gacggtggag tgaggacgat agagaccaaa gactagagac cggagatcgg
     2161 agatcagagg tcaaggggca gtcgaacgcc gatccttctc ccaagga
//
LOCUS       DASRZS010000656         6577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3838822, whole genome shotgun sequence.
ACCESSION   DASRZS010000656 DASRZS010000000
VERSION     DASRZS010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3838822"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFH83_15950"
     CDS             complement(<1..197)
                     /locus_tag="VFH83_15950"
                     /inference="COORDINATES: protein motif:HMM:NF026171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET6487922.1"
                     /translation="MTFASRGHPDARARFREALFSGLAADGGLYHPVERPDLGPVLAT
                     MDATTPFGDLAERVIASILSP"
     gene            complement(194..1135)
                     /locus_tag="VFH83_15955"
     CDS             complement(194..1135)
                     /locus_tag="VFH83_15955"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET6487923.1"
                     /translation="MRERSSVSVFAPATVANVASGFDVLGFALQEPGDVVTLRRVGAP
                     TITVVSITGDGGRLPRDTDRNTSAVAAKGFLDKIGNPFGVEISLEKRMPLSSGLGSSA
                     ASAVAAVFGANLLAGSPLARTELLPFTMEAERVACGSAHADNVAPSLLGGFALIRSYH
                     PLDVVKLPVPEGLACAVVHPHTEVRTEDARRILKKEIRLSDAVQQWGNLAALVAALFS
                     GDLALLGRSLEDVVAEPARSLLIPGFPAVKSAALAAGALGCSISGSGPSVFALCASAQ
                     SAAAAGKAMVEAFAGAGLQSDLYLSSVNTRGPVELSS"
     gene            complement(1132..2547)
                     /locus_tag="VFH83_15960"
     CDS             complement(1132..2547)
                     /locus_tag="VFH83_15960"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET6487924.1"
                     /translation="MRVLKFGGSSLADGERIKAVTTIVASAAEAERLAVVLSAMKGVT
                     DLLISAARKAEEGPDGFRPILDRIRSQHVDAVRYLFAAADQSAALTPIALLCNELEEI
                     LHGVELIRECSPRTLDLIMSFGERLSCDLASAYFRTQGLAAETVDAREIVLTDDRFGS
                     AAVDFPGSYARIEKRLTAVPGIPVVPGFIGATDRGVTTTLGRNGSDYTASIIGAGVGA
                     REIEIWTDVDGVLSADPRLVPEAFVIPTISYEEAMELSYFGAKVIHPYTMVPAVEKDV
                     PLLIKNSLNPAAPGTRIVAGAVMRREGGRPVTGIASIEGISLVNIEGGGMMGIPGFAA
                     RTFAALAKAGINIIMISQASSEHTICLVFRTGEGEKALAALNQELAFELSTRRIERFE
                     LLSDLVVVSAIGENMHGTPGIAGRVFSALGREGINILVIAQGSSERNISFVVQKKDHL
                     SALRTIHAEFLSGARRASASA"
     gene            complement(2554..2967)
                     /locus_tag="VFH83_15965"
     CDS             complement(2554..2967)
                     /locus_tag="VFH83_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET6487925.1"
                     /translation="MKFHTDYLTFNTRKEREYINITDRVEQSVRQSGCREGMVLVSAM
                     HITAGVYVNDAEQGLIQDIDEWLDRLAPTGPDYRHHRTGETNGDSHLKNLLIGHEVIL
                     PVTAGKLDLGPWQQVYYAEFDGQRPKRVIIKVMGE"
     gene            complement(3056..3937)
                     /locus_tag="VFH83_15970"
     CDS             complement(3056..3937)
                     /locus_tag="VFH83_15970"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6487926.1"
                     /translation="MGINISRFYQSRVNVFLIRHLGRTAAQKYLGLVGAAYYLFSRHE
                     KRIIERNVRDVLSGKDEAYVRRVIRDTFRGITTHYYEKMCSAYLDYHSVSRYVDERFE
                     VRGGELLREALRSGKGCILVTAHWGAVEFIPWVLHRKGFPASIILECATTKLERCLQG
                     QVRHADAELISTGCGSIFRRAIQSLGANRVLMTECDEVDAWRKRPNHTIRLFGKDLYF
                     DNAIDVLAHRTGAPVVAAFLERLDGGRYALNVEDISVRRSPPSTAQECLKLLQQYIDH
                     DPAQWYQWKKWGEMKSA"
     gene            complement(4014..5876)
                     /locus_tag="VFH83_15975"
     CDS             complement(4014..5876)
                     /locus_tag="VFH83_15975"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6487927.1"
                     /translation="MSARPGIRTATEGNNVSCLNSKAIIGYFRRNCPERLPDLLAGLG
                     EPWASMPQLDAYLSDETNWIPSGLVVTLFQNARAITGNENIAFDIGFDSVLHREYSYW
                     QRIFVRIFGSPEGVLRRLNQLNNRLNTTKVVELIATAPGRAVIRWHWREGVVTSKDAC
                     AYNRGIYAALPTIWELPAATVEERTCWFEGGPYCQILFTWSLSRGRFRSVLSNLITRK
                     STLLTALEEIERDKTLLKEKFDELHRVNQELSQKVTMLRSINDAARTLVSVSDTQLVL
                     QQTLKPIVEVFGYDRALIMMVDDKEEFLEYRYGVGESVDAMEKMKGYRVPLSRDQNLM
                     IRAMRAGKPVLVKDVSAAGLNPSNFILADFHPSSFVICPLIAEGKAIGLLGADRRVER
                     GHLSSTDAESLTIFATTIATAFQRARMDEELKGSYVSSVRALVQAIEEKDTYTRGHSE
                     RVSRMAVEVGRVLGLSDQEVNYLRFGGILHDVGKIGIPESIVRSPKPLTEAEFRIIKK
                     HPLKGVEILQPIAFMRSRMHIIRNHHERWDGKGYPDQLAQDEIPLGAQIVAVADAFDA
                     MTSSRPYRRGMPAKQAAREIQRSEGTQFSRTIAEAFREALERLSAESTTEVGKT"
     gene            6096..>6577
                     /locus_tag="VFH83_15980"
     CDS             6096..>6577
                     /locus_tag="VFH83_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487928.1"
                     /translation="MSIPLRFSFSHGARTGRTASDSLVLALEAGGRVGYGEAVVREYV
                     SGSLGSGETFRQESARLAGELIQQFRDRDLSWPEIRDVLLDLPCEERSLPLLCALEAA
                     LLDCACHLDGGDAYRLMGVEPAREHMVYSAVLPILPIQLAERYLSKYVEIGFQHYKVK
                     V"
BASE COUNT         1086 a         2118 c         2186 g         1187 t
ORIGIN      
        1 tccggtgaga ggatgctggc gatcacgcgc tcggcgaggt ccccgaaggg cgtggtcgcg
       61 tccatggtgg ccagcaccgg tccgagatcc ggccgctcca cgggatggta gagcccgccg
      121 tcggccgcaa gcccggagaa gagcgcctcc cggaaccttg cgcgagcgtc cgggtggccg
      181 cggctggcga acgtcatgag gagagctcga ccggaccgcg cgtgttcacc gaggacaggt
      241 acaggtcgct ctgcagaccg gcgccggcaa aggcctccac catcgccttg ccggccgcgg
      301 cggccgactg cgcgctggcg cacagcgcga acaccgaggg gcccgagccg gagatggagc
      361 atcccagggc cccggccgcg agggccgcgg acttcacggc gggaaagccg gggatgagga
      421 gcgaccgcgc cggctccgcc acgacgtcct ccagcgaacg gcccaggaga gcgagatcgc
      481 cggaaaagag agcggccacc agggccgcca ggttgcccca ctgctgcacg gcgtccgaga
      541 ggcggatctc ctttttcagg atccgccgcg cgtcctcggt tcggacctcc gtgtgcggat
      601 gcaccacggc gcaggccagc ccttccggca ccgggagctt caccacgtcg agcgggtggt
      661 agctgcggat gagcgcgaag ccgccgagga gggagggcgc cacgttgtcc gcgtgggcgc
      721 tgccgcaggc cacccgctcc gcctccatgg tgaagggcag aagctcggtg cgcgccagtg
      781 gactgccggc caggagattg gccccgaata cggccgccac cgcgctggca gccgagctgc
      841 ccagacccga ggaaaggggc atccgcttct ccagggagat ctccacgccg aacgggttgc
      901 cgatcttgtc gaggaagccc ttcgccgcga cggcggaggt atttcggtcc gtgtcccggg
      961 gaagcctgcc gccgtctccc gtgatggaga ccacggtgat cgtaggggcg cccacccgcc
     1021 gcagcgtgac cacatccccc ggctcttgga gcgcgaaccc cagcacgtcg aacccgctgg
     1081 cgacgttggc aacggtggcc ggcgcgaaca ccgagacgga ggagcgctcc ctcatgcgga
     1141 ggcggaggcg cgccgggcgc cggagaggaa ctcggcgtgg atggtacgga gcgcagagag
     1201 gtggtccttc ttctggacca cgaaggagat gttgcgctcc gaggagccct gggcgatcac
     1261 caggatgttg atgccttccc gtcccagggc gctgaacacg cggccggcga tcccgggcgt
     1321 tccgtgcatg ttctccccga tcgcggacac caccaccagg tcgctcagaa gctcgaaccg
     1381 ttcgatgcgc cgcgtggaga gctcgaaggc aagctcctgg ttcagggcgg ccagcgcctt
     1441 ctccccctcc cccgtgcgga acacgagaca gatggtgtgc tccgaggacg cctgggagat
     1501 catgatgatg ttgatcccgg ccttggccag cgcggcaaac gtgcgggcgg cgaagcccgg
     1561 gatgcccatc atgcctccgc cctcgatgtt gaccagggag atcccctcga tggaggcgat
     1621 cccggtgacc gggcgcccgc cttcgcgccg catgaccgct ccggcgacga tccgcgtccc
     1681 cggggcggcg gggttcaggc tgttcttgat gaggagcggg acgtccttct ccaccgccgg
     1741 caccatcgtg taggggtgga tcaccttggc gccgaagtag gagagctcca tcgcctcctc
     1801 gtagctgatc gtcgggatca cgaacgcttc cggcaccagc cgcgggtccg cggagaggac
     1861 cccgtccacg tccgtccaga tctcgatctc ccgggccccg acgcccgcgc cgatgatgga
     1921 ggccgtgtag tccgatccgt tgcgccccaa ggtggtggtc acgccccggt cggtggcgcc
     1981 gatgaacccg gggaccacgg ggatgccggg gacggcggtg agacgcttct cgatccgcgc
     2041 gtagctcccc gggaagtcca cggccgcgga accgaaccga tcgtccgtca gcacgatctc
     2101 ccgcgcgtcc acggtttccg ccgcaaggcc ctgggtccgg aagtacgccg atgccaggtc
     2161 gcaggagagc cgctcgccga agctcatgat caggtccagg gtccgggggg agcactcccg
     2221 gatgagctca acgccgtgga ggatctcctc cagctcgttg cacaggagtg cgatgggcgt
     2281 cagcgccgcg gactggtccg ccgccgcgaa gaggtagcgt accgcatcca cgtgctggga
     2341 acggatccgg tccaggatgg ggcgaaaccc atccggtccc tcctccgcct tgcgcgcggc
     2401 cgagatcagg aggtccgtga cccctttcat cgccgagagg accacggcca ggcgctccgc
     2461 ctccgcggcg ctggccacga tggtcgtgac cgccttgatc ctctccccgt ccgccaggga
     2521 gcttccgccg aacttcagga cgcgcaccgt tcctcattcc cccattactt tgatgatgac
     2581 ccgtttgggt ctttggccgt cgaactccgc gtagtacacc tgctgccacg gacccaggtc
     2641 cagcttcccc gcggtcaccg gcaggatgac ctcgtgaccg atcagcaggt tcttgagatg
     2701 gctgtctccg ttggtctccc cggtgcggtg gtgccggtag tccgggccgg taggcgccag
     2761 gcggtccagc cactcgtcga tgtcctggat gaggccctgc tcggcgtcgt tcacgtacac
     2821 gccggccgtg atgtgcatgg cggagaccag gaccatgccc tcccgacagc cgctctgccg
     2881 aacggactgt tcgacgcggt cggtgatgtt gatgtattcc cgctccttgc gggtgttgaa
     2941 cgtcaggtag tcggtgtgga acttcacggg cgctccgggt gagccgacaa tacaccgaag
     3001 agcgccttgc ggtctacgag agcgccgcgc ggatctgatc tgagccgccc ggccgtcagg
     3061 ccgacttcat ctccccccac ttcttccact gataccactg agcagggtcg tggtcgatgt
     3121 actgctgcag gagcttgagg cactcctggg ccgtactggg cggagaccga cggaccgaga
     3181 tatcttccac gttcagcgca tagcggcccc cgtcgagcct ctcgaggaat gccgccacga
     3241 cgggggcacc ggtccggtga gccagcacgt cgatggcgtt gtcgaagtac aggtccttgc
     3301 cgaacaggcg gatggtgtgg ttggggcgct ttctccaggc gtccacctcg tcgcactccg
     3361 tcatgagtac ccggttggcg cccagggact ggatggccct gcggaagatg gagccgcacc
     3421 cggtggaaat gagctccgcg tcagcgtggc ggacctgccc ctggaggcag cgctccagct
     3481 tggtcgtggc gcattccagg atgatgctgg cggggaatcc cttgcgatgg aggacccacg
     3541 ggatgaactc cacggcgccc cagtgcgcgg tgaccagaat gcagcccttt ccactcctca
     3601 gcgcctcgcg caggagctct ccgcccctga cttcgaagcg ctcgtccacg taacggctta
     3661 cgctatggta atcgaggtat gcggagcaca tcttctcata gtagtgggtc gtaatgcctc
     3721 ggaacgtatc ccggatgacc cggcgcacgt aagcttcgtc cttgcccgat agcacgtctc
     3781 ggacgtttct ttcgatgatt cgcttctcgt ggcggctgaa aaggtagtag gccgccccaa
     3841 cgaggcccag gtacttctgg gctgccgtac gccccaagtg gcggatcagg aaaacgttta
     3901 cccgggactg gtagaacctg gaaatgttga tgcccatccc atgcctcccg cgcccacgtt
     3961 ttcgcgtata ttatcacgag aggaatggag aagggaacta ttttttattt tttttaggtt
     4021 tttccaactt ccgtggtaga ctccgcggac aggcgctcca gcgcctcccg aaaggcttcg
     4081 gcgatggtcc gggagaactg ggttccttcg ctccgctgga tttctcgggc cgcctgcttg
     4141 gctggcatcc ccctccgata cggccgggag gaggtcattg cgtcaaaagc atccgctacc
     4201 gccacgatct gggctcccag agggatctca tcctgggcaa gctggtccgg gtagcccttg
     4261 ccgtcccagc gctcatggtg atttcggatg atgtgcatcc ggctccgcat gaaggcgatg
     4321 ggctgaagaa tctcgacccc cttcagggga tgtttcttta tgatccggaa ctcggcctcg
     4381 gtcaagggtt tggggcttcg gacgatggat tccggaatcc cgatcttgcc cacgtcgtgc
     4441 aggatgcctc caaaccgcag gtagttgacc tcctggtcgc tcagaccgag gaccctgcct
     4501 acctccaccg ccatccggga cactcgctcg gagtggcccc gggtgtacgt atccttctcc
     4561 tcgattgcct ggaccagcgc gcggacggag ctcacgtagc tccccttcag ctcctcgtcc
     4621 attcgggcgc gctggaacgc cgtggctatc gtggttgcga agatcgtgag ggattccgcg
     4681 tccgtcgagc tgaggtgtcc ccgctctacg cgccggtccg cgcccagaag cccgatggcc
     4741 ttgccctcgg cgatcagggg gcagatgacg aaggagctgg gatggaagtc cgccaggatg
     4801 aagttggaag ggttgagccc ggcggcgctt acgtccttca cgaggaccgg tttcccggcg
     4861 cgcatggcgc ggatcatgag gttctggtcg cgagagagcg ggacgcggta gcccttcatc
     4921 ttctccatgg catcgacgga ttctccgacg ccgtagcggt attccaggaa ctcttccttg
     4981 tcgtcgacca tcatgatcag ggcgcggtcg tacccgaaga cctccacgat gggcttgagc
     5041 gtctgctgga ggaccagttg cgtgtccgag acggagacca gggtccgggc cgcgtcgttg
     5101 atggaacgga gcatcgtgac cttctggctc agctcctggt tgacccggtg cagctcgtcg
     5161 aacttctcct tcagaagagt cttatcccgc tcgatctctt ccagagcggt gagcagggtg
     5221 gacttgcggg tgatcaggtt ggaaaggacg ctgcggaacc ttcctctaga gaggctccag
     5281 gtgaagagga tctggcaata ggggccgccc tcgaaccagc aggtccgctc ctccacggtt
     5341 gccgcgggta gctcccagat cgtgggcagg gcggcgtaga tgccccggtt ataggcgcag
     5401 gcatccttgg aggtgaccac gccctcccgc cagtgccagc ggatgaccgc ccggcctggg
     5461 gccgtggcga tcaactcgac caccttcgtg gtgttgaggc ggttgttgag ctggttgagg
     5521 cgtcggagga ccccctccgg agagccgaag atccggacaa agatccgctg ccagtagctg
     5581 tactcccggt gcaggaccga atcgaacccg atgtcgaagg cgatgttctc gttccccgtg
     5641 atggcgcggg cgttctgaaa gagagtcacc accaggccgc tggggatcca gtttgtctca
     5701 tcagaaaggt aagcatcgag ttggggcatg gaggcccagg gctcgccaag gccggccagc
     5761 aggtcgggga gtcgctcggg gcaattccgg cggaagtagc cgatgatcgc cttgctattc
     5821 aggcaactta cgttgttgcc ctccgtcgcc gtccggatgc ccggtcgtgc agacattggt
     5881 agaaacctac ccccttcgag gactggttac acggggaatt ggatactttt ggattccctg
     5941 cagtataaac ccgatccggc cggcgtgcca gggaaacttt tccgatgctt tgtgcgtcgg
     6001 ttaccgccgg atcccttgca acccacgaga ccacgaggta gcatcgccct cgagggttgg
     6061 cgagcgtgag gattgggcac gcgaaactgc atttcttgag cattccgctc aggttcagct
     6121 tcagtcacgg ggctcgaacc ggacgcacgg cgtcggattc cctggtcctc gcgttggagg
     6181 ccggcggacg tgttggctat ggcgaggcgg tcgttcggga gtacgtgtcc gggtctctcg
     6241 gcagcggcga aacgtttcgc caggaaagcg caaggctggc cggggagctc atccagcagt
     6301 ttcgggatcg ggacctgtcc tggccggaga tccgtgacgt ccttctggac ttgccctgtg
     6361 aggagcgctc tctgccgctc ctgtgcgccc tggaggccgc cctcctggac tgcgcctgcc
     6421 acctcgacgg cggcgacgcg taccggctca tgggagtcga gccggcccgg gagcacatgg
     6481 tgtacagcgc cgtcttgccc atccttccga tccagcttgc cgagcggtac ctgtcgaagt
     6541 acgtggagat cgggttccag cactacaagg taaaagt
//
LOCUS       DASRZS010000657         5396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1914970, whole genome shotgun sequence.
ACCESSION   DASRZS010000657 DASRZS010000000
VERSION     DASRZS010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914970"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /gene="ilvC"
                     /locus_tag="VFH83_15985"
     CDS             <1..889
                     /gene="ilvC"
                     /locus_tag="VFH83_15985"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET6487929.1"
                     /translation="VGLRPGSPSAKKASEAGLPVMDTAAAVAAAEVIMILIPDHLQGE
                     VYKKDIAPELKAGKTLLFAHGFNIHFGQIKPPPGVDVAMIAPKGPGHTVRRQYELGFG
                     VPCLIAIAQDPSGKARDTALSYAMAIGGAKAGVIETSFREETETDLFGEQAVLCGGVT
                     HLMQAGFETLVEAGYAPEMAYFECIHEMKLIVDLIYEGGLSLMRYSISDTAEYGDYTV
                     GPRIITEQTKKEMKKVLSEIQSGDFAKRWMAENASGRKSFLAMRQEGAAHQLEKVGEK
                     LRSMMPWIAKNRLVDKSKN"
     gene            complement(886..1638)
                     /locus_tag="VFH83_15990"
     CDS             complement(886..1638)
                     /locus_tag="VFH83_15990"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6487930.1"
                     /translation="MVEAYPEAKRAALTWIKPGVSRYSDRGIAMNVHELLEESDFFRT
                     LSAAGRRAVASICVPKVLRKRETLFREGEQGHSMFILAQGTVQLFKSSEDGKEVVIKL
                     LRPGEIFGEVVLFEQDRFPVSACALTPAQVFLLPRRQFDCLLQEEAFRRDFIAMLLAK
                     QRYLAERIFRLSALDVEQRFFHFLREHYGELEEYSIDITKRDFAAAIDALPETLSRLL
                     LKLRDDGTAAWDGQTLSLRRGFWRDRGAGAAS"
     gene            1637..2494
                     /locus_tag="VFH83_15995"
     CDS             1637..2494
                     /locus_tag="VFH83_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET6487931.1"
                     /translation="MSKRKIISIDEAKCNGCGLCLPNCPEGALQVIDGKVRLISDLFC
                     DGLGACIGYCPEGAISVVEREAEPYDERRVMASIVRQGANTIRAHLEHLESHGERGFL
                     ELALAYLKENGIPVPQGFPAKQAAACRAGCPEEPSSKPGAPSDLAAKGAVEAEPASAL
                     ANWPVQLHLLSPMAPQFRGKDVLLSADCVAFAVGDFHRKFLAGRTLAIACPKLDDGKE
                     VYVEKLAALIDHAQIRSLTVTIMQVPCCRGLLSLVQKAMANAHRTIPVRVVTVAIADG
                     SVLAEEAIA"
     gene            complement(2544..3359)
                     /locus_tag="VFH83_16000"
     CDS             complement(2544..3359)
                     /locus_tag="VFH83_16000"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6487932.1"
                     /translation="MEAKDFYDTLGEDYDRMVSWEGRLAREDAFFRGLFDEHGVRRVL
                     DAACGTGMHAIAFARQGRSAEGADLSPVMVDHARRNAAAAGVTVTFTVAGFGDLAARL
                     GGPFDAVTCLGNSLPHLPDDASLDAAFRDFARALRPGGLLVIQNRNYDRVLRERSRFM
                     PVAARTDDEGETLFLRITDFPGPIASAPASGATAADARSEESIAFTIVTLKKRAGSWS
                     QSTRTTPLRALRRATLERAARDAGFTDVRLFGSYDRAAFDAPGTGDLILTATR"
     gene            3463..4593
                     /gene="alr"
                     /locus_tag="VFH83_16005"
     CDS             3463..4593
                     /gene="alr"
                     /locus_tag="VFH83_16005"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET6487933.1"
                     /translation="MSGLVWVELDGGALEHNLKELRKGSAPGVLSCAVVKSNAYGHGI
                     AQVSKLLPSAEWFGVNSLDEGLELRRLGVERPILLLGPVPLADLGEAVEAALCLTVYN
                     RETIERLSAIRSARGPARVHVKVDTGTSRQGVLPENLEEFLKLVLAAPGVRLEGLSTH
                     YANIEDTLNHAFAQLQIGRFQQALVTAEKMAGRIPYVHTACTAAALLFQQTHFTMLRT
                     GIGLYGLWPSRETMLSARERGGEVPSFRPVLRWKTRIAQIKTVPQGSYVGYGCSYRTT
                     RATLLGVLPVGYADGYTRMVGNRGHVLVNGRRAPVIGRICMNLCMVDLTDLPSARLEE
                     EVVLLGTSGDETISAETMAEWTGTINYEVVTRISPLLPRKVV"
     gene            complement(4656..4838)
                     /locus_tag="VFH83_16010"
     CDS             complement(4656..4838)
                     /locus_tag="VFH83_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487934.1"
                     /translation="MERVQAREGLVCICAECKKVIRRIDNQSEAEAQTQRPMVSHGIC
                     PECAERLYREIFYRKR"
     gene            complement(4966..>5396)
                     /locus_tag="VFH83_16015"
     CDS             complement(4966..>5396)
                     /locus_tag="VFH83_16015"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6487935.1"
                     /translation="AWSIAPIPQLTATSSPAVNLYGASVSIPKSTPQKQLAAWLFIKW
                     LSEPKQQAAWTRVSMYFPVRKAAEPLIKDILDANRTFAVAWNLLKTANLKAEPPFAGY
                     DLVRDAIVAAYSKVLDGADVDATLAALQSQADKIYKDSTP"
BASE COUNT          932 a         1775 c         1819 g          870 t
ORIGIN      
        1 ggtcggcctg cgcccgggca gcccctcggc gaagaaggca tcggaggcgg gcctgccggt
       61 gatggacacc gcggccgcgg tggctgccgc cgaggtgatc atgatcctca tccccgacca
      121 cctgcaggga gaggtctaca agaaggacat cgctccggag ctgaaggccg gcaagacgct
      181 cctcttcgcg cacgggttca acatccactt cggccagatc aagccgccgc cgggggtgga
      241 cgtggcgatg atcgctccca aaggaccggg ccacacggtc cgccgccagt acgagctggg
      301 attcggcgtt ccctgcctca tcgccatcgc ccaggatccg tcgggcaagg cgcgggacac
      361 ggccctgtcc tatgccatgg ccatcggcgg cgctaaggcg ggcgtgatcg agacgagctt
      421 ccgggaggag acggagacgg acctcttcgg ggagcaggcg gtcctctgcg gcggggtcac
      481 ccacctgatg caggcggggt tcgagacgct ggtggaagcc ggctacgcgc ccgagatggc
      541 ctatttcgag tgcatccacg agatgaagct catcgtggac ctgatctacg aaggcggcct
      601 gtctctcatg cgctattcca tctccgacac tgcggagtac ggggactaca ccgtgggacc
      661 ccggatcatc acggagcaga ccaagaagga gatgaagaag gtcctctcgg agatccagag
      721 cggggacttt gccaagcggt ggatggcgga gaatgcctcc ggccgcaaga gcttcctggc
      781 catgcgccag gagggcgccg cccaccagct cgagaaggtg ggagagaagc ttcgctccat
      841 gatgccctgg atcgcgaaga accggctggt ggacaagagc aagaactaag aggccgcgcc
      901 cgcgccgcgg tcccgccaga atccgcggcg caggctgagg gtctgaccgt cccaggcggc
      961 tgtcccgtcg tcccgcagct tcaggagcaa acgggagagc gtctcgggca gcgcgtcgat
     1021 agccgcggcg aagtcccgct tggtgatgtc gatggagtat tcctccagct cgccgtagtg
     1081 ctcgcggagg aagtggaaga accgctgctc cacgtccagg gcggaaagac ggaagatccg
     1141 ctccgccagg taccgctgct tggccaggag catggcgatg aagtcccggc ggaacgcctc
     1201 ctcctggagc aggcagtcga actgtcttcg ggggaggagg aagacctggg ccggcgtcag
     1261 ggcgcaggcg ctgacgggga atcggtcctg ctcgaagagc accacctccc cgaagatctc
     1321 ccccggccga agcagcttga tgaccacttc cttgccgtcc tccgagctct tgaagagctg
     1381 cacggtgccc tgcgcaagga tgaacatgga gtgaccctgc tccccctccc ggaagagggt
     1441 ctcccgtttg cgaagcacct tcggcacaca gatggaggcc acggccctgc ggccggccgc
     1501 ggagagggtc cgaaagaaat ccgattcttc cagcagctcg tggacgttca tggcgattcc
     1561 tcggtcacta taccgggaaa cgccgggctt gatccacgtc aaggcggctc tcttcgcctc
     1621 ggggtatgct tcgaccatga gcaagcgcaa gatcatcagc atcgacgagg ccaagtgcaa
     1681 cggatgcggc ctgtgcctcc ccaattgccc cgagggggcc ctccaggtca tcgacggaaa
     1741 ggtgcgcctg atcagcgacc tcttctgcga cggcctgggc gcctgcatcg ggtactgtcc
     1801 cgaaggggcc atctccgtcg tggaacgcga ggcggaaccc tacgatgagc ggcgcgtcat
     1861 ggcttcgatc gtgcgccagg gcgccaacac gatccgggcc catctggagc acctggagtc
     1921 acacggggag cggggattcc tcgagctggc gctcgcttac ctgaaggaga acgggatccc
     1981 cgtcccgcag ggcttcccgg cgaaacaggc cgccgcgtgc cgcgcaggct gcccggagga
     2041 gccgtcgtcg aagcccgggg cgccctctga cctcgccgcg aagggggcgg tcgaagcgga
     2101 gcccgcatcg gcgctcgcca actggccggt gcagcttcac cttctctccc ccatggcgcc
     2161 gcagttccgc ggcaaggatg tcctgctcag cgcggactgc gttgcgtttg ccgtcgggga
     2221 cttccaccgc aagttcctgg ccggcaggac gctggccatc gcgtgcccaa agctggacga
     2281 tgggaaggaa gtctacgtag agaagctggc cgctctgatc gaccacgccc agatccggtc
     2341 cctcaccgtg accatcatgc aggtcccgtg ctgccgaggt ctcctttccc tggttcagaa
     2401 ggcgatggcg aacgcgcacc gcacgatccc ggtccgggtg gtcaccgtgg cgattgcgga
     2461 cggaagcgtg ctggccgagg aggccatcgc gtagccgtgg cggcgagagg gacgcgcgcc
     2521 gggccgatgc accggcaacc gccctaccgg gtggcggtca ggatcagatc accggtgccc
     2581 ggcgcgtcga aggcggcgcg gtcgtagctc ccgaaaagcc gcacgtccgt gaagccggca
     2641 tcccgcgctg cgcgctccag ggtagcgcgg cgaagggccc gcaagggcgt ggtgcgcgtc
     2701 gattggctcc agctcccggc gcgcttcttg agggtcacga tggtgaacgc gatggattcc
     2761 tcggagcgcg catcggcggc cgtcgcaccg gaggccgggg ccgatgcgat cggtcccggg
     2821 aaatccgtga tccgcaggaa gagcgtctct ccttcgtcgt ccgtgcgcgc cgccacgggc
     2881 atgaaccgcg agcgctcccg aagcacccgg tcatagttcc gattctgtat caccaggagg
     2941 ccgcccggtc tcagcgcccg ggcgaagtcg cggaaggccg cgtccagaga cgcatcgtcc
     3001 ggcagatgcg gcagggagtt tcccagacag gtcacggcgt cgaaaggacc acccaaccgt
     3061 gcggccagat cgccgaatcc ggcgacggtg aacgtcacgg tgactcccgc cgcggccgcg
     3121 ttgcgccgcg cgtgatccac catcaccggg ctcagatctg ccccctccgc ggacctcccc
     3181 tggcgcgcaa aggcaatggc gtgcatcccg gtgccgcagg cggcgtccag gacccgccgc
     3241 acgccgtgct cgtcgaacag cccgcggaag aacgcatcct cccgagccag ccgtccctcc
     3301 caggagacca tccggtcgta gtcctcgccc agggtgtcgt agaagtcctt cgcttccatc
     3361 accgttgctc ccgggggccg cgtgccgaac cgcccgtgcg cgcgctggtc cctttgactg
     3421 ctcgcacggg cgcatagtac cctgcggccg aacggaggat caatgagcgg cctcgtctgg
     3481 gtggagctgg acggtggtgc cctggagcac aacctgaagg agttgcgcaa gggaagcgcc
     3541 ccgggggtcc tgtcctgcgc ggtggtcaaa tccaacgcgt atggccatgg cattgcccag
     3601 gtctccaagc tgctgccctc cgcggaatgg ttcggggtca attccctgga cgaggggctc
     3661 gagctgcggc gtctcggggt ggagcgcccc attctgctgc tggggccggt gccgctggcg
     3721 gacctgggag aggcagtgga ggccgctctg tgtctgacgg tctacaaccg cgagaccatc
     3781 gagcggctgt ccgccatccg aagcgcccgg ggcccggcgc gcgttcacgt caaggtggac
     3841 acgggaacgt cacgacaggg agtgctgccg gagaacctgg aagagttcct gaagctggtc
     3901 ctggcggctc ccggtgtccg gctggaaggc ctctccaccc actacgcgaa catcgaggat
     3961 accctcaacc acgcgttcgc gcagctccag atcggcaggt tccagcaagc cctcgtcacg
     4021 gcggagaaaa tggctggccg gatcccctac gttcatacgg cgtgcacggc cgccgccctg
     4081 ctcttccagc agacccactt cacgatgctt cgcaccggca tcggtctgta cggactgtgg
     4141 ccgtcgcgag agaccatgct ctccgcccgc gagagagggg gagaggtccc cagcttccgg
     4201 ccggtgctcc gctggaaaac ccgcattgcc cagatcaaga ccgtccccca gggcagctac
     4261 gtcggatacg gatgctcgta ccgcaccacg cgagccacgc tgctgggcgt cctgcccgtg
     4321 gggtacgcgg acggctacac ccggatggtt ggcaaccgcg gccacgtgct cgtgaacgga
     4381 cgtcgggcgc ccgtgatcgg ccggatctgc atgaacctct gtatggtgga cctgacggac
     4441 ctgccatcgg cccgattgga ggaagaggtg gtgctgttgg gtacctccgg tgacgagacg
     4501 atcagcgcgg agacgatggc cgaatggacc ggcaccatca actacgaggt ggtcacccgg
     4561 atcagccccc tcctgccccg aaaggtcgtc tgaggccgga cgttcgatcc ggcccagatc
     4621 gggtcgtcag agatgccgcc gctcgccgtc tgcggtcacc gttttcgata gaagatctcg
     4681 cggtagagtc tctcagcgca ttctggacag atcccatggc tcaccatggg gcgctgggtt
     4741 tgtgcctctg cctccgactg gttgtcgatc cgtcggatca ctttcttgca ctcggcacaa
     4801 atgcagacaa gcccctcccg cgcctgcact cgctccatat cccctcccga taaagagtcc
     4861 atatactgtg taacaccgcg agataactgt caatggagga atttgagagg cttgaggcgg
     4921 gcctcccgag gcacggctcg ggaggtccag ccaggggcgc caggctcagg gtgtggagtc
     4981 cttgtagatc ttgtccgcct ggctctgcag ggcagccagc gtcgcgtcca cgtccgcccc
     5041 atccagtacc ttgctgtagg ccgcaacgat ggcgtcccgc accaggtcgt agccggcaaa
     5101 gggcggctcc gccttgaggt tggcggtctt gagcaggttc caggccacgg caaaggtgcg
     5161 attggcgtcc aggatgtcct tgatcagcgg ctcggccgcc ttgcgcaccg ggaaatacat
     5221 ggacaccctg gtccaggcgg cctgctgttt cggctcgctc agccacttga tgaacagcca
     5281 cgccgcgagc tgcttctgag gggtggactt ggggatgctc acgcttgcgc cgtacaagtt
     5341 gacggcgggt gacgaggtgg ccgtgagctg cgggatgggc gcgatgctcc aggcaa
//
LOCUS       DASRZS010000658         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4225719, whole genome shotgun sequence.
ACCESSION   DASRZS010000658 DASRZS010000000
VERSION     DASRZS010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4225719"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VFH83_16020"
     CDS             complement(<1..1271)
                     /locus_tag="VFH83_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487936.1"
                     /translation="MVWRKREGDSEQVQKLFLEEEFRGDRLVNNCRFFILFVLLVLAL
                     VKNGFPIRFDRALIFTLQILVVGYVYTGVLFFLYRRHVYHPALKFVSMTLDITLAVLA
                     TMVYKLEPPEEYALIFMLARASIVYWFIIWSLLRYNLWLSLYSGVLGAVEYFFLVIRG
                     NDLLGIPFSFTTRDGIVHVSAFSTSEETLKLVYLVAAGVLAAVVAIRLRNLVVDSMRK
                     EHDKDELQAQNQLITAVNQENRKYLDNVGEGLLLIDGSYRISDQYSRFLTGLFGTNEV
                     AGRSFVDFMYPNAATQSAERAELEKFLSIVFTNTTASFDMIMNVNPLSSKTIQVRTES
                     GRIEERVVDATFHRITKDGKVDTVLVVFQDRTAVERAQRELEQEKQAHESEMESISAI
                     LKTGPTALKDFVDECRKGLLTVLQSLPNLAER"
     gene            complement(1274..1729)
                     /locus_tag="VFH83_16025"
     CDS             complement(1274..1729)
                     /locus_tag="VFH83_16025"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HET6487937.1"
                     /translation="MKAQYINPFLVASLGLFREYLGVQCEAGEPFLNKETQRLPEVSG
                     IIGLAGDTVGAIVLSFERDTAIKMVSKLAGKPYPALGNEVIDGVGELVNIIAGNAKRD
                     LVDYRLVISLPGVIVGENYKINWPQGVAVITIPFHSELGHFSVSVSLRD"
     gene            complement(1748..2122)
                     /locus_tag="VFH83_16030"
     CDS             complement(1748..2122)
                     /locus_tag="VFH83_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6487938.1"
                     /translation="MRILIIDDAMVMRNIHKNVLREHGLGDDDFREAGDGDTALQIAS
                     AETIDLFLVDWNIPKLNGLEFVRKIRSMEKYAATPVIMITSEAAKYNVMEAIEAGTTD
                     YLVKPIKAALLWDKLSKYVGAH"
     gene            2447..>2700
                     /locus_tag="VFH83_16035"
     CDS             2447..>2700
                     /locus_tag="VFH83_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487939.1"
                     /translation="MSRKTTLTILLILAVPQILAAQRIVSAEEFFSDLSTTFGTIKDY
                     MATVTFTQGKGVSRGRLYYKSPLFLRIDFDAPPRQVINFDG"
BASE COUNT          488 a          868 c          811 g          533 t
ORIGIN      
        1 cgctcggcga ggttgggcag gctctggagc accgtgagga gacccttgcg gcactcatcc
       61 acgaagtcct tcagcgccgt gggccccgtc ttgaggatgg ccgagatgga ctccatctcc
      121 gattcgtgcg cctgcttctc ctgttccagc tcgcgctggg cccgctcaac ggcggtccgg
      181 tcctggaaga ccaccaggac cgtatccacc ttcccgtcct tggtgatccg gtggaacgtg
      241 gcgtccacga cccgctcctc gatcctcccc gactcggtcc gaacctggat ggtcttggac
      301 gagagcgggt tgacgttcat gatcatgtcg aagctcgccg tggtgttggt gaatacgatg
      361 gagaggaact tctcgagctc ggcccgctcg gcggactggg tggcggcatt cgggtacatg
      421 aaatcgacga agctcctgcc ggccacctcg ttggtcccga acagcccggt caggaacctg
      481 gagtactggt cgctgatccg ataggagccg tcgatcagga gcagtccctc accgacgttg
      541 tccaggtatt tgcggttctc ctggttgacc gccgtgatga gctggttctg cgcctgcagc
      601 tcgtccttgt cgtgctcctt gcgcatggaa tccacgacga ggttccgaag ccggatggcg
      661 acgaccgccg ccagaacgcc ggccgccacc agatagacca gcttgagggt ctcctccgag
      721 gtggagaacg cggagacgtg gacgatgccg tcccgcgtcg tgaaggagaa gggaatgccc
      781 agcaggtcgt tgccccggat caccaggaag aagtactcca ccgcgccaag gacccccgag
      841 tacagggata gccagaggtt gtagcgcagc aaggaccaga tgatgaacca gtagacgatg
      901 gaggcgcgcg ccagcatgaa gatgagggcg tactcctcgg gtggctccag cttgtagacc
      961 atcgtggcca gcaccgccag cgtgatgtcc agcgtcatgc tgacgaactt cagcgcggga
     1021 tggtagacgt gcctgcggta caggaagaag aggactccgg tatagacgta cccgaccacg
     1081 aggatctgga gggtgaagat cagcgctcga tcgaagcgga tcgggaaccc gttcttcacc
     1141 agggccagga ccagcaggac gaacaggatg aagaatcggc agttgttgac cagccggtct
     1201 ccccggaact cctcttcgag gaacagcttc tggacctgct cggaatcccc ttcgcgcttt
     1261 ctccacacca tggctagtcc ctgagcgaca cgctcaccga aaagtggccg agctccgagt
     1321 ggaaggggat ggtgatcacc gcgacgccct gcggccagtt gatcttgtag ttctccccca
     1381 cgatcacccc gggcagcgag atcaccagcc ggtagtccac caggtcccgt ttggcgttgc
     1441 cggcgatgat gttcacgagc tccccgacgc cgtcgatgac ctcgttgccc agcgcggggt
     1501 agggcttgcc ggccagcttg gagaccatct tgatggccgt gtcccgctcg aagctgagca
     1561 cgatggcgcc gacggtatcc cccgccagcc cgatgattcc ggacacctcc ggcagacgct
     1621 gggtctcctt gttcaggaag ggctcacccg cttcacactg gacgcccagg tactcccgga
     1681 acagtcccag ggaggccacc aggaacggat tgatgtactg tgccttcatt cgtctctccc
     1741 gagctgccta gtgtgccccc acgtacttcg atagcttgtc ccagagcaac gcggccttga
     1801 tgggcttcac caggtagtcc gtggtgcctg cctcgatggc ctccatgacg ttgtacttcg
     1861 ccgcctcgga ggtgatcatg atcaccggcg tggccgcata cttctccatg cttcggatct
     1921 tccgcacgaa ctccagacca ttcaacttcg ggatgttcca gtccaccagg aagaggtcga
     1981 tggtctccgc cgaagcgatc tgcagggcgg tatcgccgtc cccggcctct cggaaatcgt
     2041 cgtccccgag cccgtgctcg cggagcacgt tcttgtggat gtttcgcatc accatcgcgt
     2101 cgtcgatgat caggattctc acggttttgt ccgcctccaa cgttcggctt tgcgcccaac
     2161 ggccaatgat agttcgacct ggacccgttt tccgctcggt tcagcaccca tggctcggcg
     2221 aggagcgccc ggtgcggcac gccggagatc tgcgccggtg cacgggatac gtgtgcggta
     2281 ttcctcaatc ataggaaagg taccaccgat tgtccagagg caccgtgttg attgccaggc
     2341 gcttttcccg aatgccgcgc gccagacccg ggctccgccc gcgcggttcc tgcgtccttc
     2401 gcgtcgcctt gacaaagaga tgcgcgtggc tactataggg tcagccttga gccgcaagac
     2461 cacgctaacc atcctcctga ttctcgcggt gccgcagatc cttgctgccc agcgaatcgt
     2521 gtccgcggag gagttcttct ccgacctgtc caccaccttc gggaccatca aggactacat
     2581 ggcaaccgtg acgttcaccc aggggaaggg cgtctcccgc ggccgtctgt actacaagtc
     2641 tcctctgttc ctgcggatcg acttcgatgc tcctccccga caggtcatca acttcgacgg
//
LOCUS       DASRZS010000659         3104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4987430, whole genome shotgun sequence.
ACCESSION   DASRZS010000659 DASRZS010000000
VERSION     DASRZS010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4987430"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFH83_16040"
     CDS             <1..363
                     /locus_tag="VFH83_16040"
                     /inference="COORDINATES: protein motif:HMM:NF016501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HET6487940.1"
                     /translation="QKIDLWIWGSNYNYWLDLYLRDYQGIDHVLHFGSLRFVGWKDLT
                     VDIPSSIPQSRTYIPRYEGMVMTKLVMWTAPDEKVDDFFLFVDEINCLTDIFESRFDG
                     EDLADPTYLNNLWQQGTK"
     gene            376..1095
                     /locus_tag="VFH83_16045"
     CDS             376..1095
                     /locus_tag="VFH83_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HET6487941.1"
                     /translation="MKTRTALTLLAFFVGFCTPAFSQNVAAGEPNPADVGKDQAQQLL
                     EEVSVDKFEDAAFWYAAMPLDQGIVTTKRLAGSPAGKTPLPGETALNIKAQDQYVLGV
                     KVSFFHRGPATFSIHPLNPIPVEGITKTISVWVVGRNYPHTLKVDFLDYNGQPQEITM
                     GTLNFVGWKKLTVAVPPSILQTQYHYGGARNGIAITGLTVYTDDLDTYGTYYVYFDDM
                     RAVTDLFGESKRDSDDMSDGW"
     gene            1177..1998
                     /locus_tag="VFH83_16050"
     CDS             1177..1998
                     /locus_tag="VFH83_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487942.1"
                     /translation="MTPEEELKRLNRLIHQYETVYRTTPDLGQRGRVERQLKELRSYR
                     DKILAVNVIESPEAEAEPAAESELADYPLLTSLIGQNLARAQDTAVKPFSPEGVEPTA
                     SQREMHNLALYVDLFEHEFIPFLTEKHLKLDFKFSMDRDAFYAGLQALIRRIADYREE
                     QSRLAEGRVGREIEVDVRKRGSKLKRLIEVEAARLFRAVERFADELATDARGPGVKCL
                     NNRGQVTFDSIEGNRLLQGKAVTEALTELHDLAAEAVDYLNVPDIEQENERADRH"
     gene            1982..2362
                     /locus_tag="VFH83_16055"
     CDS             1982..2362
                     /locus_tag="VFH83_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014487002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET6487943.1"
                     /translation="MQIDIDANTTIGEESFFQGRFAIRGSLRIDGRFEGQALLVDQLQ
                     IGPKGRVKTNITATSVVVEGVSIGNITASRRILLLSTARVLGELKTPELIIQDGVILE
                     GRCTISHVQIENTPEYVMSLYDRK"
     gene            2445..2515
                     /locus_tag="VFH83_16060"
     tRNA            2445..2515
                     /locus_tag="VFH83_16060"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2477..2479,aa:Gln,seq:ctg)
     gene            2550..2621
                     /locus_tag="VFH83_16065"
     tRNA            2550..2621
                     /locus_tag="VFH83_16065"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2583..2585,aa:Glu,seq:ctc)
     gene            complement(2590..>3104)
                     /locus_tag="VFH83_16070"
     CDS             complement(2590..>3104)
                     /locus_tag="VFH83_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487944.1"
                     /translation="EIAKLADRRDVISALVDEYAAHNREALPELEEKRKAILHDLAGL
                     DAERKRLSRWLLRTEPTAQAVAFVNAQIEAVSEKETRLQESQWAIEDQINELQKQTYD
                     AETISTQLKDFVANFPGLDQGERKLLVESLVEGVEIGRNKRVAASLRPPFAFGFFSPD
                     LAPRGIEPLF"
BASE COUNT          597 a          970 c          987 g          550 t
ORIGIN      
        1 cagaagatcg atctctggat ctggggctcc aactacaact actggctgga cctgtacctc
       61 cgggactacc agggcatcga tcacgtcctg cacttcggct cccttcgctt cgtcggctgg
      121 aaggacctca cggtggacat cccatcctcg attccccagt cgcggaccta catcccgcgg
      181 tacgagggaa tggtgatgac gaagctggtc atgtggacgg cgccggatga gaaggtcgac
      241 gatttctttc tcttcgtcga tgagatcaac tgcctgaccg acatcttcga gtccaggttc
      301 gacggtgagg acctcgcgga cccgacgtac ctgaacaacc tctggcagca gggaaccaaa
      361 taggagaatc gggagatgaa gactcgaacc gcgctgaccc tgctcgcgtt tttcgtcggg
      421 ttctgcacgc ctgcgttctc ccagaacgtc gcggcgggag agcccaaccc cgcggacgta
      481 ggcaaggacc aggcccagca gcttctggag gaggtctccg tagacaagtt cgaagacgcg
      541 gccttctggt acgcggccat gccgctggac cagggcatcg tcactaccaa gcgtctggcc
      601 ggttccccgg cgggcaagac gcccctcccg ggagagacgg ccctcaacat caaggcgcag
      661 gaccagtacg tgttgggcgt gaaggtgagc ttcttccacc gcggcccggc caccttctcc
      721 atccatccgc tgaaccccat ccccgtcgaa gggatcacca agaccatctc cgtgtgggtc
      781 gtgggacgca actacccgca caccttgaag gtggatttcc tggactacaa cgggcagccg
      841 caggagatca ccatggggac gctgaacttc gtcggctgga agaagctcac ggttgccgtc
      901 cctcccagca tcctgcagac gcagtaccac tacggcggcg cccgcaatgg gattgccatc
      961 accggtctga ccgtgtacac ggatgacctg gacacctacg gaacgtacta cgtctacttc
     1021 gacgatatgc gggcggtcac ggacctgttc ggcgagtcca aacgggactc ggacgacatg
     1081 tccgacggct ggtgagccgc cagcgtcgct gaagcgcctg cggctacgcg gccccggggc
     1141 tatcccggag ccggagcgcc gtggtagagt cccgccatga cacccgagga ggagctgaag
     1201 cggctgaatc ggctcatcca ccagtatgag acggtctatc ggaccacgcc cgacctgggg
     1261 cagcgcggtc gggtggaacg acagctcaag gagcttcgca gctaccgcga caagatcctc
     1321 gcggtgaacg tgatcgagag ccccgaggcc gaggcggagc cggcggcgga gtccgagctg
     1381 gccgactacc cgctcctgac gagcctgatc ggccagaacc ttgcgcgggc acaggacacc
     1441 gcggtcaagc cgttctcccc agagggcgtg gagccgaccg cctcccagcg ggaaatgcac
     1501 aacctggccc tctacgtcga cctcttcgag cacgagttca tcccgttcct cacggagaag
     1561 catctcaagc tcgacttcaa gttctccatg gaccgtgacg ctttctacgc cggcctgcag
     1621 gccctgatcc ggagaatcgc cgactaccgg gaggagcaga gcaggctcgc ggagggccgg
     1681 gtgggtcgtg agatcgaggt ggacgtccgc aagcgcggct ccaagctcaa gcggctgatc
     1741 gaggtggaag cggctcgtct ctttcgggcc gtggagcgct tcgcggacga gctggccacc
     1801 gacgcgcgcg gcccgggggt aaagtgtctg aacaaccgcg gccaggtgac ctttgacagc
     1861 atcgagggga accgcctgct gcagggcaag gcggtgacgg aagcgctgac cgagctgcat
     1921 gatcttgcgg cggaggcggt ggactacctc aacgtgccgg acatcgagca ggagaacgag
     1981 cgtgcagatc gacattgatg ccaataccac catcggcgag gagtccttct tccagggccg
     2041 gttcgccatt cgaggcagcc tccgaatcga tggccgattc gagggccagg cgctgctggt
     2101 ggaccagctc cagatcgggc ccaaaggccg cgtcaagacg aacatcacgg ccacgagcgt
     2161 ggtggtggaa ggcgtctcca tcggcaacat cacggcctcc cgacggatcc tcctgctttc
     2221 cacggcccgc gtcctggggg agctgaagac gccggagctg atcatccagg atggcgtgat
     2281 cctggagggt cggtgcacga tcagccacgt gcagatcgag aacacgccgg agtacgtcat
     2341 gtccctgtac gaccggaagt gagcgggcgg cccgggcacg ctcttggttc aggcttgctc
     2401 ttgacaagac ccggcttggt ccattacata tgaggcggaa gcgattcccg gtagtgtaac
     2461 ggtagcacaa aagactctgg atctttttgt gggggttcga atcctccccg ggaaagaccg
     2521 cggcgctggg acaaagggcg gcgcgacgtg gccccttcgt ctatcggtta ggacatgaga
     2581 ttctcaatct taaaagaggg gttcgattcc cctaggggct aaatcgggag agaaaaaccc
     2641 gaaagcaaag ggtggtcgca gagaagcggc cacccttttg tttctcccga tctctacccc
     2701 ctctaccagg gactccacga gcagcttcct ttccccctgg tcgagacccg ggaagttggc
     2761 cacgaagtcc ttgagctggg tcgagatggt ctccgcgtca taggtctgtt tctgcagctc
     2821 gttgatctgg tcctcgatgg cccactggct ctcctgcagc ctggtctcct tctcggagac
     2881 ggcctctatc tgggcgttca caaaggcgac ggcctgggct gtgggctcgg tccggagcag
     2941 ccagcgggag agcctctttc gctctgcgtc gaggccggcc aggtcgtgaa ggatggcctt
     3001 ccgcttctcc tccagctcgg ggagggcttc gcggttgtgc gccgcgtatt catccacgag
     3061 ggcagagatg acgtcccgac ggtcggcaag cttggcgatc tccc
//
LOCUS       DASRZS010000660         4722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3841251, whole genome shotgun sequence.
ACCESSION   DASRZS010000660 DASRZS010000000
VERSION     DASRZS010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3841251"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VFH83_16075"
     CDS             complement(<1..884)
                     /locus_tag="VFH83_16075"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6487945.1"
                     /translation="MKAVIIGTSGHIDLALEVRDRLPQVSFVGIAPGSADEDARDFFV
                     ELLEPSLIPFYDDYRRMLDRERPELAIVAPFFFLQSRIACECLERGIHVFLEKPMSVS
                     LEQLERLRRAYALGKAKLCPMLPYRYHPEFHAAWRALNEGRIGEPLLISAQKSYKLGT
                     RHPMYTHRATYGGTIPWVAIHGMDWIHWYSGGRIVEVSAGHTTVGNRGHGEMESSGAC
                     LYRLANSGVATLTFDYFRPRAAPSHGDDRVRIAGEKGVIEVAQGEATLTTHDSPPERM
                     AKEEPLSIFQEFIRSLEDG"
     gene            1147..1962
                     /locus_tag="VFH83_16080"
     CDS             1147..1962
                     /locus_tag="VFH83_16080"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6487946.1"
                     /translation="MQAEHSLEDGDLTIAQFVNHAQALALLLRGDSDLLLSGTSQGWE
                     NRLDGSPIVMIDTGIWGISSLVGRDAGIRSMADLRGKRVALPFPGSPLDFQTRALLAH
                     EGLDPDHDVSLSYAPFAQSVPRLLSGQLDAAALPEPLASTVVAKNGLLRLLDYSQAWA
                     SFTGGDRQSPQVSLFTTETFARDHRDLLARVVSAWQAATARVIQSPAEMAARFASALS
                     ADPGILEQATRNTLFAVPSVQDNKTRVLAYYQTVSRYFPAPAKPLDQGFFVEP"
     gene            1967..3457
                     /locus_tag="VFH83_16085"
     CDS             1967..3457
                     /locus_tag="VFH83_16085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6487947.1"
                     /translation="MPAAGTRTGRRTGGRYPALTAAGVAILLAAWALASVVAGTYFVP
                     PPWTTVVDTAVLLAGRFAWSQILITVLRVCIGFAVGFLAGVALGIAIGARRGLEALFR
                     PAVLFFQGMPPLLWAIPLVALMGIGHLPAILVIALITFPVVAVTIGEGMSSLPNELGE
                     MLSVFAPGFSPRLRELILPHLAPFLAASLKAGVVLAVKASVTAEYFGANDGIGFQIQA
                     AYMSIRIRSLFAWAVVLVLLITVANRLLPQIRRLRPLVERLFIETGPLVCRIEDERAL
                     DRALRADGKTSRGGIQLRSVSFAYPRQPFLLNDLSLVVPPGGIVVVRGDSGAGKTTLL
                     KLTAALLRPAKGSITRPARIAFVFQDDRLLPWRSVLENVALPLRYQGYPEREALCFAS
                     ILSRQAGLDGDGKTRPEELSGGMKRRAALARCFARLPDAILLDEPFSGLHREARRELW
                     KWFLAMHARRPVPTVIVTHFPEEVRRRGSPVVTLRGRPARLEVTRS"
     gene            3454..>4722
                     /locus_tag="VFH83_16090"
     CDS             3454..>4722
                     /locus_tag="VFH83_16090"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HET6487948.1"
                     /translation="MSEASLRSIALRIRGAALDSADAGTAVRRALAPAPAGLTALGKL
                     WPVSGRLVLIAVGKAAPRMAATALEILGSRVDRGIVVAPRGARLEAPDRRLLLMESSH
                     PLPDENGVQAARAVLALVQELTDRDLCLVLVSGGGSSLLPLPAPPLTLADKVQTTNLL
                     LRCGADIREINTVRSHLSAIKGGRLAEQCRGSMVTLALSDVVGDPLPFIASGPTVANP
                     TTYQDALTVLDRYGLRGSAPAPALQRLLEGAEGVIPECPKELPSRFQAGIVAGNGVAL
                     DAAAREASGAGFQPMVLTSYLGGEARDAGRFLAAVVREILAHGRPVKPPACLLMGGET
                     TVTVHGNGTGGRNQELALSAAIELAGSFGWLIASYATDGKEGNSEAAGATASGETVER
                     GRRSGLDPRRCLAANDSTTFLTASGDLIVTG"
BASE COUNT          699 a         1628 c         1564 g          831 t
ORIGIN      
        1 ccgtcctcaa gggatcgaat gaactcctgg aagatcgaaa gaggctcctc cttggccatg
       61 cgttcgggtg gggagtcatg cgtggtcagc gtggcctccc cctgcgccac ctcgatcact
      121 cccttctctc cggcgatgcg gacccggtca tcgccgtggc ttggggccgc gcggggacgg
      181 aagtaatcga aggtgagcgt cgcaacgccg gagttggcca gccgatacag acaggcgccg
      241 gagctctcca tctcgccgtg gccccgattg cccacggtgg tgtgacctgc ggagacctcg
      301 acgatcctgc caccgctgta ccagtggatc cagtccatcc catggatggc gacccagggg
      361 atggtgccgc cataggtggc ccggtgggtg tacatgggat gtcgcgttcc gagcttgtag
      421 gacttctggg cggagatgag caacggctcc ccgatcctgc cttcgttgag cgcccgccac
      481 gcggcgtgga actcgggatg gtagcgatac ggcagcatgg ggcagagctt tgccttcccc
      541 agcgcgtagg cccgccgcag ccgctccagc tgctccaggg aaacggacat cggcttctcg
      601 agaaacacgt gaatgccgcg ctccaggcat tcgcaggcga tccgggactg gaggaagaag
      661 aaaggggcca cgatggcgag ctcgggcctc tcccggtcca gcatgcggcg ataatcgtcg
      721 tagaacggga tgagggaagg ctcgagcagc tcgacgaaga agtcccgggc gtcctcgtcc
      781 gcggagccgg gagcgatccc cacgaagctt acctgaggca gacggtcgcg gacctccaag
      841 gcgagatcga tatgcccgct tgtgccgatg atcaccgcct tcatgggaat gcctcgacgc
      901 caactatagc agagcagtca cgccccatcc aatagagccc cgtggcggtt cttgcccatc
      961 cccggcaaaa cgaatagggt gtcgggcgtc atgcgcctgt ctaccggatt ggtccgctcg
     1021 cggggtctgg gttgcccggt cctggccctg ctgctctgtg ccgctcccgc cttttctgcc
     1081 gggagtgcgc ctgccgcgct tcgagtcgag gtcccaccgt ctaccgcggc cctcccgttc
     1141 cttctcatgc aggcggagca ctcccttgag gacggggacc tcacgatcgc ccagttcgtc
     1201 aaccacgcgc aggccctggc tctgcttctc cgaggagatt cggacctgct cttgagcggg
     1261 accagtcagg ggtgggagaa ccggctggac ggcagcccga tcgtcatgat cgataccggc
     1321 atctggggca tctcctcgct ggtgggccgg gatgccggca tcaggagcat ggcggacctc
     1381 aggggcaagc gcgtggcgtt gcccttcccg ggctcgccgc tcgatttcca gacccgcgcc
     1441 ctcctggcgc acgagggcct cgacccggac catgacgtga gcttgagcta cgcgcctttc
     1501 gcccagagtg taccgcgcct tctctcgggt cagttggatg ctgccgcgct gcccgaacca
     1561 ctggcgagca cggtcgtggc aaagaacggc ctgctgcggc tcctggacta ctcccaggcg
     1621 tgggcgtcgt tcacgggagg ggatcgccag tccccgcagg tgagcctctt caccacggag
     1681 accttcgccc gggaccaccg ggacctcctg gcacgggtcg tctccgcctg gcaggcggca
     1741 acggcccggg tcatccaatc tccggcggag atggccgccc gattcgcctc cgcgctctcc
     1801 gcggaccccg ggatcctgga gcaggcgacg cgcaacaccc tcttcgccgt gccttcggtc
     1861 caggacaaca agacccgggt ccttgcctac taccagaccg tatcccgcta cttcccagcg
     1921 cctgcgaagc cgctggatca agggttcttc gtggaaccct gaagccatgc cggccgcagg
     1981 gacacggacg ggacgccgga ccgggggccg ctaccccgcc ctcaccgcgg ccggtgtggc
     2041 catcctgctg gctgcctggg ccctggccag cgtcgtcgct gggacctact tcgttccccc
     2101 accctggacc accgtcgtcg atacggccgt cctcctggcc ggcaggtttg cgtggtccca
     2161 gatcctgatc accgtgctcc gcgtctgcat cgggttcgcc gtcggcttcc tggccggagt
     2221 cgcgctcggg atcgccatcg gcgctcgccg gggcctggaa gcgctcttcc gcccggcggt
     2281 cctcttcttc cagggaatgc cccctctctt gtgggctatc ccgctggtgg cgcttatggg
     2341 catcggccac ctgccggcga tcctggtgat cgcgctgatc acctttcccg tcgtcgccgt
     2401 caccatcggg gaaggaatgt cttccctccc gaacgagcta ggagagatgc tttccgtttt
     2461 cgcgcccggc ttctcgcctc ggctgcggga gctcatcctt ccgcatctgg ctccgttcct
     2521 ggcggcctcc ctgaaggcgg gggtggtcct cgcggtcaag gcatcggtga ccgcggagta
     2581 cttcggcgcc aacgacggga tcgggttcca gatccaggca gcctacatgt ccatccggat
     2641 ccgaagcctc tttgcctggg ccgtggttct ggtcctgttg atcaccgtgg caaaccggct
     2701 cctcccgcag atccgacggt tgagacccct cgtagaacgg ttgttcatcg agacgggtcc
     2761 gctggtctgc cggatcgaag acgagcgcgc gctcgatcgc gccctgagag ccgatgggaa
     2821 gacatcccgg ggcgggatcc agctccgctc ggtgagcttc gcgtacccgc gccagccgtt
     2881 cctgctgaac gacctgtccc tggtggtgcc gcccggcggc atcgtggtag tccgtggaga
     2941 ttcgggtgcg ggcaagacca cgcttctgaa gctgaccgcg gcgcttctac gccccgcgaa
     3001 ggggagcatc acccgcccgg cgcggattgc cttcgtgttc caggacgacc gcctcctccc
     3061 ctggagaagc gtgctggaga acgtggcgct tccgcttcgc taccaggggt accccgaacg
     3121 tgaggccctc tgcttcgcct cgatcctgtc gcggcaggcc ggcctcgatg gagacggcaa
     3181 gacgcgtccc gaggagctct cgggcggcat gaagcggcgt gccgcccttg cccgatgctt
     3241 cgcgcggttg cccgatgcca tcctgctgga cgagccgttc agcgggctgc accgcgaggc
     3301 gaggcgcgag ctctggaagt ggttcctggc gatgcacgcg cggagacccg tgcctaccgt
     3361 gatcgtgacg cacttccccg aggaggtccg ccgccgcggg tcgcccgtgg tcactctgcg
     3421 gggacgaccc gcccggctcg aggtgacacg atcgtgagcg aggcatcgct tcgcagcatc
     3481 gccctgcgga tccgaggcgc ggcgctggat tccgcggatg cggggaccgc ggtccgacgc
     3541 gcgctggcgc cggcccctgc gggtctgacc gccctgggaa agctgtggcc ggtctccggc
     3601 cggctcgtgc tgatcgcggt cgggaaggca gccccacgca tggccgccac ggcgctcgag
     3661 atcctgggta gccgcgtgga tcgagggatc gtcgttgcgc ccaggggcgc tcgccttgaa
     3721 gcgcctgacc gtcgactcct cctgatggaa tccagtcatc ccttgccgga tgagaatggc
     3781 gttcaggccg cccgcgcggt cctggccctg gtccaggagc taacggaccg ggacctctgc
     3841 ctggttctgg tctcaggcgg gggatcgagc ctccttcccc ttcccgcgcc tcccctcacg
     3901 ctggcggaca aggtccagac aaccaacctg ctcttgcgat gcggggcgga catccgggaa
     3961 atcaacacgg tccgctccca cctcagcgcg atcaagggag gtcgcctggc cgagcagtgt
     4021 cgtggttcca tggtcaccct tgccctgtcg gacgtggtgg gggatcctct tccgttcatt
     4081 gcctcggggc cgaccgtggc caacccgacc acctaccagg atgcgcttac ggtgttggac
     4141 cggtatgggc ttcggggttc agctccggca ccggccctgc aacggctgct ggagggagct
     4201 gagggcgtca tacccgaatg cccaaaggag ctgccgtcca ggttccaggc aggcatcgta
     4261 gcaggcaacg gcgttgctct cgatgccgcg gcccgggaag cctcaggcgc cggtttccag
     4321 ccgatggtcc tcacgagcta tctcggtggc gaggcccgag acgctggccg cttcctggcg
     4381 gcggtagtcc gggagatact ggctcacggc cgacccgtga agccaccggc gtgcctcctc
     4441 atgggagggg agaccaccgt cacggtgcac gggaacggga ccggaggccg caatcaagag
     4501 ctcgcgcttt ccgccgccat cgagcttgcc ggcagcttcg gatggctgat cgcttcctat
     4561 gccaccgatg gcaaggaagg aaactcggag gccgccggcg ccactgccag cggagaaacc
     4621 gtggagcgcg gccggcggtc cgggcttgac cctcgccgat gcctggctgc gaacgactcc
     4681 acgacgtttc tcaccgccag cggcgacctg atcgtcaccg gc
//
LOCUS       DASRZS010000661         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3074790, whole genome shotgun sequence.
ACCESSION   DASRZS010000661 DASRZS010000000
VERSION     DASRZS010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3074790"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..746
                     /locus_tag="VFH83_16095"
     CDS             216..746
                     /locus_tag="VFH83_16095"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET6487949.1"
                     /translation="MKILLLLEADSATEVARQVRSGIEALAAAGGCTVTTVEVRRDEV
                     PPCFGCLACMTRHPGICVHQRAFESILAQSGDCDVAVFLTAVVFGTFGSAMKNLIDRG
                     GLIIRNHRRCRQIFIGYGAEVTEEERRTFLDLTAAHRGKADIVHLNVRESIEVYFAGS
                     KAEADSICRNIESGAA"
     gene            743..1369
                     /locus_tag="VFH83_16100"
     CDS             743..1369
                     /locus_tag="VFH83_16100"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET6487950.1"
                     /translation="MKACLISGSLRGAQSSSHQFLQDLSARLTAAGIDLERGTVRADP
                     RSPYLEGFPGAMHEADAIVFAFPLFTYSLPGALTRLLEDLSARAGRKARTVPIKVFAI
                     INCGFPRPRICADAIRVIRNFCQRLGYDYRFSVAIGGGPVTVLTRKVPVLNRDLKRAY
                     DRIVDEIRGVDPGGTGDVYVKPVVPERILLWIKDRYEQKTPTLRREPA"
     gene            complement(1317..>2051)
                     /locus_tag="VFH83_16105"
     CDS             complement(1317..>2051)
                     /locus_tag="VFH83_16105"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HET6487951.1"
                     /translation="MTGSNANLDQDVSWRSFYAAGSIAALAFVILVVVPLVLVFAAPQ
                     VPASGGAAVLQYIASHKAVYLAELVCFVGLSVPALVVFCAAGISLKDASRSLAALGAL
                     LGVASEIVALSLGSSPQSLNGGLVLLSSQYAAGGDAQRAVLATAAEGIIASTNAVSWA
                     GILTALGILLLSIAMRNGSYPRWVSYLGIVTGILGVPSEAFRPSVGPAYAVYGILLPV
                     WFGAVGWCLLTRARDAGSASSARSDP"
BASE COUNT          417 a          644 c          662 g          328 t
ORIGIN      
        1 tccaacgcgc gcacttatca ctggaagccg ctcccatacg agggaaatct accggactat
       61 ggcgatttcc ggaagctatg atgcctttgt cagcagcctg ttagccccaa tcatgggcgc
      121 ccgaagcgcg gcgatcccct agagcacgga aataaccctc cgaatacatc ctttggcccc
      181 tgacaacggc cgcgccaggg ggcatggttt ggggcatgaa gattctcttg ctgttggaag
      241 cagacagcgc cacggaggta gcgcggcagg ttcgttcggg gatcgaggcg ctcgccgctg
      301 ctgggggatg caccgtcact accgtcgaag tcagaaggga cgaggtgccc ccgtgctttg
      361 gctgtctggc ctgcatgacc agacacccgg ggatctgcgt acatcagcgg gcattcgaga
      421 gcatcctagc ccagagtgga gactgcgacg tggcggtctt tctgaccgcg gtggtcttcg
      481 ggaccttcgg gtccgccatg aagaacctga tcgaccgggg cgggctcatc attcggaacc
      541 accgtagatg ccgccagatc ttcatcggat acggcgcgga ggtaaccgaa gaggagcggc
      601 ggacctttct ggacctgacc gccgcacatc gggggaaggc ggatatcgtg cacctcaacg
      661 tccgggaatc catcgaggtc tatttcgccg ggtcgaaggc ggaagccgac tccatctgcc
      721 ggaacatcga gtcaggtgcg gcatgaaggc atgcctcata agcgggagcc tgcgaggggc
      781 gcaatcctcc agccaccagt tcctacagga cctttcggca cgattgacgg ccgccgggat
      841 cgacctggag agagggacgg taagggccga tccgcggagc ccctacctag aggggtttcc
      901 cggcgccatg cacgaagcag acgcgatcgt gttcgcgttc ccgttgttca cgtacagcct
      961 cccgggagct ctcacccgac ttctggagga tctctccgcg cgcgccgggc ggaaagctcg
     1021 cacggttccc atcaaggtct ttgcgatcat caactgtgga ttcccccggc ctcggatctg
     1081 cgccgatgcc atccgcgtca tccgcaactt ctgccagcgc ctgggatatg actaccgatt
     1141 ctcggtcgcc atcgggggag ggcccgtcac ggtgctcact cgcaaggtgc ctgtgctgaa
     1201 ccgggacctg aagcgggcat atgaccgaat cgtcgacgag attcgtggcg tcgatccggg
     1261 cggtaccgga gacgtgtacg tgaagccggt ggtgcccgag cggatcctcc tatggatcaa
     1321 ggatcgctac gagcagaaga cgccgaccct gcgtcgcgag cccgcgtgag caggcaccac
     1381 cccacggccc cgaaccagac gggaagcaga ataccgtaga cggcgtacgc gggccccacg
     1441 gaaggccgaa acgcttcgga aggcacgccg aggatcccgg tgacgatgcc caggtacgag
     1501 acccaccgag ggtaacttcc gttgcgcatg gcgatggaca gtaggaggat gccgagggca
     1561 gtcaggatac cggcccacga gaccgcattt gtcgacgcga tgatgccctc cgcggccgtg
     1621 gcaagaaccg cgcgctgggc atcaccgcca gcagcgtact gggagctgag gaggacgagc
     1681 ccgccgttca gggactgcgg gctgctgccc agagacagag caacgatctc cgacgccact
     1741 cccaggagag cgccgagcgc cgcgaggctc ctgctggcgt ccttcaggga aatcccagcc
     1801 gcgcagaaca cgaccagggc agggacgctc aggccgacga aacacaccag ctccgccagg
     1861 tagacggcct tgtgggaagc gatgtattgc agtacagcgg caccgccgga ggctggaacc
     1921 tgaggcgcgg caaagaccag caccagcggc actacgacca ggatgacgaa cgccagagcc
     1981 gcaatacttc cggctgcata gaaggatcgc caggatacgt cctggtccag attcgcgttg
     2041 ctccccgtca t
//
LOCUS       DASRZS010000662         2956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2286890, whole genome shotgun sequence.
ACCESSION   DASRZS010000662 DASRZS010000000
VERSION     DASRZS010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2286890"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFH83_16110"
     CDS             <1..279
                     /locus_tag="VFH83_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6487952.1"
                     /translation="NELIPENIRVNTVDQGLILTPDWKKSAGILTQGTSTTVDQYLDK
                     IAKDYTPIGRFATPEELAHFFVFLCSPRASYCVGASYLVDGGWLKTVD"
     gene            323..1453
                     /locus_tag="VFH83_16115"
     CDS             323..1453
                     /locus_tag="VFH83_16115"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET6487953.1"
                     /translation="MKITRVEVIRSRRPRKLPQEYRAAWFEPDGEPVTTYGFAFYRVH
                     TDAGVSGVGPYGGDPDAFALRALIGSDPFYVERFYAAAMNGREMTFHRGSYGGLEVAL
                     WDIMGKVTGQPIHRLLGAYRDRVPVYAATSRLLSESQHVEQVLELRQMGFRAVKLRLH
                     RQDPRDDLKVVQAVREAAGDDVRIIVDANQNHKSTRYPHWTRQTALSMARELQGLGVY
                     FLEEPLPRHDLEGLAQLAAAVEIPIAGGEHATSIVELREHVLRGAYDILQPDVTLGDM
                     GISGSKKLSIIAGYHGRQIVPHVSGLGSFALNFPSMLQVASTMDNCPILEYPFDPPIL
                     TVETEQRILRTPLLVDKDGCVAVPAGPGIGVEIDEKAVAEDL"
     gene            complement(1475..2170)
                     /locus_tag="VFH83_16120"
     CDS             complement(1475..2170)
                     /locus_tag="VFH83_16120"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET6487954.1"
                     /translation="MSPKKPSSKRPAAKRAAAAVTLGIPVGQRTVPLPAPSSSRGLSV
                     FSALAERKTIRTISGRGISLQLLSDLLWSACGINRESGPFGIPGRTAASASNSQEIDL
                     FVALELGVYRYVPIPHSLLLVSSEDLRPLAIGPGQGMAGAKAPVRLIYVVDIARFAKA
                     GFQEPGLWNQEIQKSYYFVDTGLIAGNVYLFAASRGLAAWFHNCNRKELADKLRLRPD
                     QHALFGQTIGYAE"
     gene            complement(2181..>2956)
                     /locus_tag="VFH83_16125"
     CDS             complement(2181..>2956)
                     /locus_tag="VFH83_16125"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6487955.1"
                     /translation="RARRAFVKTSGNTILITGGATGIGYALAEAFLAHGNQVAICGRR
                     EDRLREAAKSHPELRTLAADVATEAGRAALVQWATSQLKGLNVLVNNAGIQRDIDFTK
                     GIGDYRSGPSEIGINFEAPILLSGMLIPHLAGKPEAAIVNVSSGLGFVPAARMPVYSA
                     TKAGLHAFSMALRHQVKPLGIKVYELIPPAIDTELNPEGRAARGGWKVDLKPPEFVAA
                     VMKGLASDSFEIGFGTTERSIRASREELEKSFQQMNSRW"
BASE COUNT          519 a          965 c          959 g          513 t
ORIGIN      
        1 aacgagctca tcccggagaa catccgcgtg aacacggtgg accaggggct gatcctgact
       61 ccggattgga agaagtccgc gggcatcctg acccagggaa cctcgaccac ggtggaccag
      121 tacctggaca agatcgccaa agactacacg ccgatcgggc gattcgcgac gccagaggag
      181 ctggcccact tcttcgtgtt cctgtgctct ccgcgggcca gctattgcgt cggcgccagc
      241 tacctggtgg atgggggttg gctcaagacc gtcgattgac ggatcggtcc ttggagcgtc
      301 gtgtgaggga ggccgcggag tcatgaagat caccagggtc gaggtcatcc gaagccgcag
      361 acctaggaag ctgccgcagg aataccgcgc ggcatggttc gagccggacg gtgagcccgt
      421 taccacgtac ggcttcgcgt tctatcgtgt gcacacggac gccggcgtct ccggagtggg
      481 gccgtacggc ggcgacccgg acgcgttcgc gttgcgcgcc ctgatcggat ctgacccctt
      541 ctatgtcgag cgattctacg ctgctgccat gaatggccgg gagatgacct tccatcgagg
      601 ttcgtatggc ggcctggagg tagcgctctg ggacatcatg ggcaaggtca cggggcaacc
      661 catccatcgg ctcctcgggg cgtatcgcga ccgggtcccc gtgtacgcgg ccacgagccg
      721 gctcctgtcg gagagccagc acgtggagca ggtcctagag ctgcggcaga tgggattccg
      781 agccgtgaag cttcgcctgc atcgccagga cccccgagac gacctcaagg tggtccaggc
      841 cgtccgagag gcggcgggcg acgacgttcg gatcatcgtg gacgcgaacc agaaccacaa
      901 gtccacgcgg taccctcact ggacgcggca gaccgcgctg agcatggcgc gggagctcca
      961 gggtctgggc gtctacttcc tggaagagcc gttgccgcgt cacgatctgg aaggactggc
     1021 ccagcttgcc gccgcggtgg aaatcccgat cgccggcggc gagcatgcca ccagcatcgt
     1081 cgagcttcgc gagcacgtcc ttcggggcgc ctacgacatc cttcagcccg acgtcaccct
     1141 gggcgacatg ggaatctcgg gcagcaagaa gctctccatc atcgccggct accatggcag
     1201 gcagatcgtg ccgcatgtct cggggctggg aagcttcgcg ctcaacttcc cctcgatgct
     1261 ccaggtagcg tcgaccatgg acaactgccc gatcctggag tacccgttcg acccgcctat
     1321 cctcaccgtg gagaccgagc agcgaatcct gaggacgccg cttctcgtgg acaaggacgg
     1381 ttgcgtcgcc gttcctgccg ggccggggat cggcgtggag atcgacgaga aggcggtagc
     1441 cgaagacctg tgatcgaggc ctggccgaca gtcgctactc ggcgtacccg atggtctgac
     1501 cgaacagggc gtgctggtcc ggcctcagcc gcagcttgtc ggcgagctcc ttgcggttgc
     1561 agttgtggaa ccaggccgcc agccctcggg aggccgcgaa gagatagacg ttccccgcga
     1621 tgagccccgt gtccacgaag tagtaagatt tctggatctc ctggttccac agtcccggct
     1681 cctggaaccc ggccttggcg aaccgggcga tgtccaccac gtaaatgagc cgtacgggag
     1741 cctttgcacc cgccattccc tgacccggtc cgatggcgag cggccgcagg tcctcggacg
     1801 ataccaggag cagcgaatga gggatcggga cataccggta gacccccagt tccagcgcca
     1861 cgaacaggtc gatctcctgg gagttgctgg cggatgccgc cgtccttccc gggatcccga
     1921 acggaccgga ttcacggttg atgccgcacg ccgaccagag gaggtcagac aggagctgga
     1981 gagagatccc gcggccgctg atggtacgga tcgtcttgcg ctccgccaac gctgaaaaga
     2041 cggacagacc ccgactcgac gaaggcgcgg gaagcggcac ggtcctctgc ccgaccggga
     2101 ttcccagcgt caccgcggca gcggctcgtt tggccgcggg cctcttgctg gacggcttct
     2161 tcggcgacac ggctacgctc ctaccagcgg ctgttcatct gctggaagct cttttccagc
     2221 tcttcccggg aagcacggat ggaccgttcc gttgtgccga acccgatctc gaaggagtcc
     2281 gatgccagac ccttcattac ggctgccaca aactccggtg gcttgaggtc caccttccat
     2341 ccgcctcgag cggcccggcc ctccggattc agctccgtat cgatggcggg cgggatcagc
     2401 tcgtacacct tgatccccag tggtttcacc tgatgccgca gggccatcga gaaggcatgc
     2461 aggccggcct tggtggcgct gtacacgggc atgcgggccg cggggacgaa acccaggccc
     2521 gaagacacat tgacgatggc tgcctcgggt ttgcccgcca gatgcgggat cagcatcccc
     2581 gagagaagga tcggagcctc gaagttgatt ccgatctcac tggggccgga ccggtagtcg
     2641 ccaatcccct tggtgaagtc gatgtccctc tgaatgccgg cgttgttgac caggacgttc
     2701 aagcccttta gctgggacgt cgcccactgc accagcgccg cgcgtccggc ctccgtggcc
     2761 acgtcggccg caagggtacg gagctccgga tggctcttgg cggcttcccg gagccggtcc
     2821 tccctcctgc cgcagatggc cacctggttc ccgtgagcca gaaaggcctc cgcaagggcg
     2881 tacccgatgc ccgttgcgcc gcccgtaatc aggatcgtgt tgcccgaggt tttcacgaac
     2941 gccctccttg cccgcg
//
LOCUS       DASRZS010000663        10416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1918089, whole genome shotgun sequence.
ACCESSION   DASRZS010000663 DASRZS010000000
VERSION     DASRZS010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10416
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918089"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="VFH83_16130"
     CDS             complement(<1..1354)
                     /locus_tag="VFH83_16130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6487956.1"
                     /translation="MAAETLLQIKDLKTWFHTDAGTVRAVDGVDLLIRRNETLGVVGE
                     SGCGKSVTALSIMQLIDAPGRIESGEILFDGQDLRKKSRREMQHIRGNKISMVFQEPM
                     TSLNPVHTIGDQITEAITFHQRLDKREAMRKAVRMLELVGIPDPSQRVLEHPYQLSGG
                     MRQRVMIAMALSCNPSLLIADEPTTALDVTIQAQILQLMRSLKSDLGASIMIITHDLG
                     VIAEIADSVVVLYAGKVVEYSDVHSLFATPRHPYTIELLDSIPRVTDEPGRRLQPIEG
                     SIPDVMHLPRGCSFSPRCRFAVEHCRKREPAVQTLADGRQVRCWMYSKDHASLFPPPD
                     SVRARKADARGAKTEATEPGILLQVRDLTKHFPVKGGVFQTVVGQVKAVDGVSFDIRR
                     GETLGLVGESGCGKSTAGRLILRLQEPTSGQVRFEGRDLLGMGERELRAQRRNLQIIF
                     Q"
     gene            complement(1383..2450)
                     /locus_tag="VFH83_16135"
     CDS             complement(1383..2450)
                     /locus_tag="VFH83_16135"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET6487957.1"
                     /translation="MKVPKGKTDIELLRAICDAPGVTGFEDPIRAVLRPMFEACCDEV
                     TTDRMGNLVGLKRASRAPAGEAALKVMVSAHMDEIGFAVRHIDREGFVRVMPLGGIDA
                     RTVVSQRVIIHGREDVPGFIAPQPNWILSDEEKKRVLPVQELIVDTGLPKAEVEARVT
                     LGDPISFAAGFQDLNEHMVLGRNFDDRIGVYTLVEAMRRAGALGVDFYAVASVQEELG
                     VRGAITSAYAIGPDVGIAIDGSLASDVPYAKEEDRHCVLGQGTGIYLIDNRTISDPAL
                     VRFLVRLAAEHDIQFQRNLGGGTDASEIQRTKAGARALTIGAPTRYMHSTTQLCHKND
                     IEETVRLLVALLENAHSAGLR"
     gene            complement(2482..3462)
                     /locus_tag="VFH83_16140"
     CDS             complement(2482..3462)
                     /locus_tag="VFH83_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487958.1"
                     /translation="MHDAENEQTGQDAEDGGALVRRRSADSPGRDALRRLLKNRLAVA
                     GAVVILVLVLAAAFAPLVAPKPYDEGDLVQNNALPGREFPLGADFMGRDMLSRLIYGT
                     RVSLTVGFIGAVTAFLIGITYGTISGFLGGRTDSVMMRIVDILYAFPTLLLIILLMVV
                     FKTTLGAASKNPVVIAMTALDHSFGGLLFIMIGIGLTSWVDMARIARGMALSLRETEF
                     VQAARALGATKGRLVLRHLMPNLIGPCIVTVTLRIPGFIGTEAFLSFIGLGVDPPTPS
                     WGMMISEGTRVMRAYPHQAIYPGLALALVMLAFNFLGDGLRDALDPRLKR"
     gene            complement(3488..4411)
                     /locus_tag="VFH83_16145"
     CDS             complement(3488..4411)
                     /locus_tag="VFH83_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487959.1"
                     /translation="MTGYIIRRLLWTIPTLLVISLITFFLAHAIPGGPFAGPELKRMS
                     PEVIENLNKFYHLKDPIWKQYGDYLWHLVQGDLGPSYASRGRTVNEIIAQSLPASAVL
                     GLFALLVAMVIGIPLGIVSALKQNTVADYLGMFVAVAGVSVPAMTLGPLMILVFALEL
                     KVLPVAQWGTWQQVVLPAFTLGFGSAAILARLTRASMLQVLREDYIRTARAKGLPERR
                     VTVHHALRNALIPVVTIIGPLFAGLVTGSMIVEQVFAIPGMGKFFTMSVMGRDYAVIM
                     GTTLLYAVFLVLANLLVDITYARLDPRIRYS"
     gene            complement(4480..6054)
                     /locus_tag="VFH83_16150"
     CDS             complement(4480..6054)
                     /locus_tag="VFH83_16150"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6487960.1"
                     /translation="MRKSRLLLVVLAALALPALAFATEINIRMAVNPDTLDPTLVYTG
                     DVNFITKMLFVNLCDFDETRAVPTPDLAKSWEVSKDGLVWTFHLRNDVQWTNGRPVTA
                     RDVEYSMKRILDPATASPLGFYMYMIKNSKAVNTGKSKDMDSVGIKALDDWTIQFTLD
                     KPLGFFPTQIRTVAFAAPKEAIDTFGARWFEPPNIISNGPYMLQSWKQNDRLVFVKNP
                     NYYDAKNVKIDKVTVFVVTDDSTAMAMYEAGQLDTTSVPSGDIDRVAKDPVLSKQTRS
                     VPQLISYMFQFNVTQPPMDNVNVRKALAAALDKESIVKYVTKGGQVAMDTVTPFGAFG
                     AVPKAAHIGIPFDPAAARGYLAKAGYPGGKGLPQITLNFNTSELNQAVAQAAQQMWKK
                     NLGIDVQLKNIEGKVYWDAITSGNLQFWRMGVNADVPDADAFVYSTGHTTDSDRILKW
                     QRKDFDAIVEKARSEPDPEVRKQLYIQAEKIMDQDEAVVIPIYSYAYVAITKPTLNRQ
                     YSSLMADTIKNWSVTQ"
     gene            complement(6218..8251)
                     /locus_tag="VFH83_16155"
     CDS             complement(6218..8251)
                     /locus_tag="VFH83_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6487961.1"
                     /translation="MRDTLLEVRNLKTQFRTEAGVVAAVDGVDFELRENETLGMVGES
                     GCGKSVTALSVLRLIDAPGRIVDGEILFRGRNLARSSPGEMRKIRGNEISMIFQEPMT
                     SLNPVQTIGTQITEALTLHQRIGKRAALHRAVDMLKLVGIPMPEIRVHEYPHQLSGGM
                     RQRAMIAMALSCNPKLLIADEPTTALDVTIQAQILDLMRKLKSQIGSAVMIITHDMGV
                     IAEMADTVVVMYAGKVVEYADVKRIFTSPRHPYTVALLESIPRLTDLRGRKLFPIAGS
                     IPDPLSPPPGCAFHPRCRFAREICRTHMPPLMTLDGGSKARCWKYDAEQGKLFATGVG
                     EDSPRVAVVGDTAPRPRGGVPLLSVRNLVKHFPIKGGVFRTTIGHVRAVDDISFDIHQ
                     GETFGLVGESGCGKTTTGRLVLRLLERTSGTVTFEGKEIFRLSRREMRPLRKDLQIVF
                     QDPYASLNPRMTVGDILRQSFIIHKVNDRAKTNERIAELLHLVGLEPEHLRRYPHQFS
                     GGQRQRIGVARAIALNPKLVVCDEPVSALDVSIQAQILNLLEELQQRMKLSYLFIAHD
                     LSVVKHIADRVAVMYLGRIVELARSEDLFASPQHPYTEALLSAVPVPEVTERRSRIVL
                     EGDVPNPSNPPPGCRFHTRCRHVMEVCRTVEPALEGVGPGHQVACHLRSAPRI"
     gene            complement(8248..9171)
                     /locus_tag="VFH83_16160"
     CDS             complement(8248..9171)
                     /locus_tag="VFH83_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487962.1"
                     /translation="MRSKGSLWRDAFRRLLRNRLAVAGGIAILAFAVIGAFAPLIAPH
                     SFAQSDFGAAFAAPSPQYPLGADFLGRDMLSRLIYGTRISLLVGVIGALVAFTIGLLY
                     GTISGYFGGRVDNVLMRIVDILYAFPGLLFIILLMVTFKSGFGNALTGGSPFVRVVAA
                     VDKSLGGMLFIIVGISLTSWIGMARLSRGMALSLRETEFIQAAKAQGAGDLRIIVREL
                     VPNLLGPLLISVTLAIPGFIGTEAFLSFIGLGVDPPTPSWGAMIADGFQAMRSYPHLA
                     LFPGIALAILMLSFNFLGDGLRDALDPRMKR"
     gene            complement(9188..10114)
                     /locus_tag="VFH83_16165"
     CDS             complement(9188..10114)
                     /locus_tag="VFH83_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009336363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6487963.1"
                     /translation="MIGYIVRRLLWLIPVLLVTTVLTFGLMHSIPGNPFQKGDRPLPP
                     QTLRQLLAYYHLDEPVYRQYLRYMANLFKGDFGPSYQYLNRTVNDIVRGGFPVSIMLG
                     VLALLVALVIGVPLGIVSALRQNTPVDYFSMFFALTGVSVPAMTMGPLLIWIFALKLG
                     ILPVARWGTVQQAILPAFTLGIGSAAILARLTRASMLQVLREDYIRTARAKGVSELRI
                     MVRHALRNALIPVVTVVGPMAAGLLTGSLVVERIFAVPGLGKYYVDAVGVRDYPVIMG
                     TTLLYAVVLVVANLLVDITYTWIDPRIKLSQR"
     gene            complement(10197..>10416)
                     /locus_tag="VFH83_16170"
     CDS             complement(10197..>10416)
                     /locus_tag="VFH83_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487964.1"
                     /translation="DQLDKLVGDAAATPNLARRKQLYAQIEKLYVQDTVAAEPLFYQA
                     VNVLTKPYLTRPFDPSTLEFWTWKTTRK"
BASE COUNT         1824 a         3281 c         3483 g         1828 t
ORIGIN      
        1 cctggaagat gatctggagg ttgcggcgtt gcgcgcgcag ctcccgctct cccatcccaa
       61 gcagatcccg accctcgaag cgaacctgtc ccgacgtggg ctcctggaga cgcaggatca
      121 ggcggcccgc cgtggatttc ccgcagcccg actcgccgac caggcccagg gtctcccctc
      181 gccggatgtc gaagctcact ccgtcgacgg cctttacctg cccgacgacg gtctggaaca
      241 ctccgccctt cacaggaaag tgcttcgtga ggtcacggac ctggaggagg atcccgggct
      301 ccgtggcctc cgtcttcgcg ccgcgcgcat ccgccttgcg ggcgcggacg ctgtcgggcg
      361 gcgggaacag ggacgcgtgg tctttcgagt acatccagca gcgcacctgg cggccgtccg
      421 caagcgtctg gaccgctggc tctcgcttgc ggcagtgctc cacggcgaat cggcaccgcg
      481 ggctgaagct gcacccgcgg ggaaggtgca tcacatcggg aatggaaccc tcgatgggct
      541 gcagtctcct ccctggctcg tcggtgacgc gagggatgga atccaggagc tcgatcgtgt
      601 aggggtggcg cggagtggcg aacaggctgt gcacgtcgct gtactcgacc accttgcccg
      661 catacaggac gaccacgctg tcggcgatct cagcgatcac ccccaggtca tgcgtgatga
      721 tcatgatgga ggcgccgaga tcgctcttca gcgagcgcat gagctggagg atctgggcct
      781 ggatggtgac atcgagggcc gtcgtcggct cgtccgcgat caggaggctc gggttgcacg
      841 aaagggccat ggcgatcatg acgcgctggc gcatgccccc ggagagctgg tagggatgct
      901 cgaggacccg ctgggaagga tccggaatgc cgacgagctc gagcatccgc acggccttcc
      961 tcatggcttc ccgcttgtcg agcctctggt gaaaggtgat ggcttccgtg atctgatccc
     1021 cgatggtgtg gaccgggttc agcgaggtca tcggctcctg gaagaccatg gagatcttgt
     1081 tgccgcggat gtgctgcatc tccctgcggc tcttcttgcg aaggtcctgg ccgtcgaaca
     1141 ggatctcgcc ggactcgatc cggcccgggg catcgatgag ctgcatgatg gaaagggcgg
     1201 tcacgctctt cccgcagcct gattccccca ccactccaag ggtctcgttt ctgcgaatga
     1261 gaaggtcgac gccgtccacg gcccggaccg tgccggcgtc ggtatggaac caggtcttga
     1321 gatccttgat ctggagcagg gtctcggcgg ccatctcgcc ttccttcgcg gtgcccctgc
     1381 gcctagcgca ggcctgcgga gtgcgcgttc tcgaggagcg ccaccagaag gcggacggtc
     1441 tcctcgatgt cgttcttgtg gcagagctgg gtcgtgctgt gcatgtagcg cgtcggcgcg
     1501 ccgatcgtca gggctcgggc ccctgccttc gttctctgga tctccgaggc atccgtgccg
     1561 cctccgaggt tccgctggaa ctggatgtcg tgctccgcgg caagccgcac caggaagcgg
     1621 acgagagctg gatcgctgat ggtccggttg tcgatgaggt agatgcccgt cccctgccca
     1681 aggacgcagt gacggtcctc ctccttggcg tacgggacat cggaggcaag gctcccgtcg
     1741 atggcgatcc cgacatccgg accgatggcg taggcggacg tgatggcccc gcggacgccc
     1801 agctcctcct ggacggaggc caccgcgtag aagtccacgc cgagagctcc ggcccggcgc
     1861 atcgcctcca ccagcgtata gacgccgatg cggtcgtcga agttcctgcc caggaccatg
     1921 tgctcgttga gatcctggaa tccggcggcg aagctgatgg ggtcccccag ggtgacgcgc
     1981 gcctcgacct cggccttcgg gagtcccgtg tccacgatga gctcctgcac ggggaggact
     2041 cttttctttt cctcgtcgct gaggatccag ttcggctggg gagcgatgaa gccgggcacg
     2101 tcctcccgcc catggatgat gacgcgctgg gagaccacgg tgcgggcatc gatgccgccg
     2161 aggggcatga ccctcacgaa gccctcccgg tcgatgtggc gcaccgcgaa gccgatctcg
     2221 tccatgtgcg cggacaccat caccttgaga gcggcttccc cggccggcgc gcgcgaggct
     2281 cgcttgagac ccacgaggtt ccccatgcgg tccgtggtga cctcgtcgca gcaggcctcg
     2341 aacatcggac ggagaaccgc gcggatggga tcctcgaaac cggtcacccc cggggcgtcg
     2401 cagatcgcgc gcagcagctc gatatcggtc ttcccttttg gaaccttcat caggatcgct
     2461 cctccctgcc ggtgtggaat tctagcgttt gagtcgcggg tccagtgcgt cccgcagccc
     2521 gtcccccagg aagttgaacg cgagcatcac cagggccagc gcgaggcccg ggtaaatcgc
     2581 ctggtgggga tacgccctca tgactcgcgt gccttcggag atcatcatgc cccagctggg
     2641 agtcggcggg tccactccca acccgatgaa gctcaggaag gcctccgttc caatgaaccc
     2701 cggaatccgc agcgtaaccg tcacgatgca cggcccgatc aggttgggca tcaggtgcct
     2761 caggacgaga cgaccctttg tcgccccgag ggcgcgggcg gcctggacga actccgtctc
     2821 ccggagggac agggccattc cgcgggcgat gcgggccatg tctacccagg aggtcaaccc
     2881 gatgccgatc atgatgaaca gcaggccgcc gaacgaatgg tcgagcgcgg tcatcgcgat
     2941 caccacgggg tttttcgacg ccgcgcccag ggtggtcttg aacaccacca tgaggaggat
     3001 gatcaggagc agcgtcggga acgcgtacag gatatcgacg atccgcatca tcaccgagtc
     3061 cgtcctgccg cccaggaatc ccgagatcgt gccataggtg atcccgatga ggaacgccgt
     3121 caccgccccg atgaatccaa cggtgagaga gacgcgcgtc ccgtagatga gcctgctcag
     3181 catgtcgcgt cccatgaaat cggcccccag cgggaactcc ctccccggaa gcgcattgtt
     3241 ctggaccagg tcgccctcgt catagggttt gggagccacc aggggcgcga aagcggctgc
     3301 gagcaccagg accaggatga cgaccgctcc cgcgaccgca agacggtttt tcagcaggcg
     3361 tcgcagggcg tcgcggcctg gactgtcggc ggaccttctg cggacaaggg cgcctccgtc
     3421 ctccgcgtcc tggcccgtct gttcgttttc cgcgtcgtgc atatcgtctc cggttgaggc
     3481 gaacggctca ggagtagcgg atgcgcggat ccagccgcgc gtaggtgatg tccaccagca
     3541 ggttcgcgag gaccagaaag accgcgtaga ggagcgtcgt tcccatgatc accgcgtagt
     3601 cccggcccat gacgctcatg gtgaagaact tccccatccc ggggatcgcg aacacctgtt
     3661 ccacgatcat cgatccggtg accagtccgg caaacagggg accgatgatg gtgacgaccg
     3721 ggatcagcgc gttcctcagc gcatggtgca ccgtcacgcg acgctccggc aggcccttgg
     3781 cccgcgcggt gcggatgtag tcttcccgaa gcacctgcag catgctggcg cgcgtcaggc
     3841 gggcaagaat ggcggcgctc ccgaagccga gggtgaaggc tggcaaaacg acctgctgcc
     3901 aggtccccca ctgggcaacc gggaggactt tgagctcgag cgcgaatacc aggatcatca
     3961 gggggcccag ggtcatggcc ggcacggaaa ccccggcaac ggcaacgaac atgccaaggt
     4021 agtcggcgac cgtgttctgc ttcagggcgg agacaatccc cagaggaatg ccgatcacca
     4081 tcgccaccag gagcgcgaag agccccagca cggcggaggc gggcaggctc tgcgcgatga
     4141 tctcgttcac cgtcctgccc cgggaagcgt aggaagggcc caggtctccc tggacgaggt
     4201 gccacaggta atccccgtac tgcttccaga tcgggtcctt gaggtggtag aacttattga
     4261 gattctcgat cacctcgggg gacatgcgct tgagctcggg accggcgaac ggcccgccgg
     4321 gaatcgcgtg cgcaaggaag aaggtgatca gggagatcac gaggagcgtg gggatcgtcc
     4381 acaacagcct tcggatgatg taccctgtca tgcctccccc ctggccgggc cgaaaaaaag
     4441 gaggccggcg gtgcgggacc gccggcctgc atcagacgct tattgcgtca cagaccagtt
     4501 cttgatcgtg tccgccatca gggacgaata ctggcggttg agcgtcggtt tggtgatggc
     4561 gacataggcg tagctgtaga tggggatcac gacggcctcg tcctgatcca tgatcttctc
     4621 cgcctggatg tacagctgct tgcggacctc gggatccggt tcagagcgcg ccttctccac
     4681 gatggcatcg aagtccttgc gctgccactt caggatccgg tcgctgtcgg tggtgtggcc
     4741 ggtcgagtac acgaaggcat ccgcatccgg aacgtccgcg ttgactccca tcctccagaa
     4801 ctgcaggttg cccgaggtga tggcgtccca gtacaccttg ccctcgatgt tcttgagctg
     4861 gacatcgatg ccgaggttct tcttccacat ctgctgcgcc gcctgggcca cggcctggtt
     4921 gagctccgag gtgttgaagt tgagcgtgat ctggggaaga cccttcccgc ccggatatcc
     4981 ggccttggcc aggtagcccc tggctgcggc cgggtcaaag gggatcccga tgtgcgccgc
     5041 cttgggaacg gcgccgaacg caccgaacgg ggtgacggtg tccattgcca cctgtccgcc
     5101 tttggtcacg tacttgacga tggactcctt gtccagcgcc gcggcgagcg cctttcggac
     5161 gttcacgttg tccatgggcg gctgcgtcac gttgaactgg aacatgtacg agatgagctg
     5221 cgggacgctc cgggtctgct tggacagcac gggatccttg gccacccggt cgatgtcacc
     5281 ggacggaaca ctggtggtgt cgagctggcc cgcttcgtac atggccatgg ccgtggagtc
     5341 gtccgtcacg acgaagacgg tgaccttgtc gatcttcacg ttcttggcgt cgtagtagtt
     5401 ggggttcttg acgaacacca ggcggtcgtt ctgcttccag gactggagca tgtacgggcc
     5461 gttggagatg atgttgggcg gctcgaacca ccgggcgccg aacgtgtcga tggcctcttt
     5521 cggcgcggcg aaggcgaccg tccggatctg ggtcggaaag aaccccaagg gcttgtccag
     5581 ggtgaactgg atggtccagt catccagggc cttgatcccc acggaatcca tgtccttgct
     5641 cttgccggtg ttgaccgcct tcgagttctt gatcatgtac atgtagaatc ccagcggcga
     5701 tgccgtggcg ggatcgagga tgcgcttcat gctgtattcc acgtcccggg cggtcacggg
     5761 cctcccgttg gtccattgga cgtcgttgcg cagatggaac gtccagacca ggccgtcctt
     5821 ggacacttcc caggacttgg cgagatccgg cgtcggcacg gcccgggtct cgtcgaaatc
     5881 gcagaggttg acgaacagca tcttggtaat gaagttcacg tcaccggtat agaccagggt
     5941 cggatccagc gtgtccgggt tgaccgccat ccggatattg atctctgtcg cgaacgccag
     6001 ggcgggcagc gccagcgccg ccaggaccac gagcagaaga cgggatttcc tcatccagca
     6061 cctccttggt aggccccctg ccggatgtct cccggcgagg actcgggcag ggttactcta
     6121 cactcccagg atgacactgt caacaaaaat aaattatggg cgtccccggt accgccgcat
     6181 tatcgttttg tagacaagat cacatacgat accgccgtca gatcctcggg gcgctgcgca
     6241 gatgacaggc cacctggtgg cccggtccca cgccctccag cgccggctcc accgtccggc
     6301 acacctccat gacgtgccgg cagcgggtgt ggaagcggca gcccggcggt gggttgctgg
     6361 ggttgggcac gtcgccctcc agcacgatgc ggctgcggcg ctccgtcacc tccggcacgg
     6421 ggaccgcgga caggagcgcc tcggtgtacg gatgctgggg cgaggcaaag aggtcttccg
     6481 agcgcgccag ctccacgatc ctgcccaggt acatgacggc cacccggtcg gcgatgtgct
     6541 tgaccacgct gagatcgtgg gcaatgaaca ggtaggagag cttcatcctc tgctgcagct
     6601 cctccaggag gttgaggatc tgcgcctgga tggagacgtc cagcgccgac acgggctcgt
     6661 cgcataccac gagcttcggg ttcagggcga tggcgcgcgc cacgccgatg cgctgtcgct
     6721 gccccccgga gaactggtgc gggtatcggc gcaggtgctc aggctccagg cctacgaggt
     6781 ggaggagctc ggcgatccgt tcgttggtct tcgcgcggtc gttcaccttg tggatgatga
     6841 agctctgccg gaggatgtcg ccaacggtca tgcgcgggtt cagggaggca tacgggtcct
     6901 ggaagacgat ctgaaggtcc ttccgcagcg ggcgcatctc tcgcctggag aggcggaata
     6961 tctctttgcc ctcgaaggtg acggtgcccg acgtccgctc gaggagccgg agcaccaggc
     7021 gacccgtcgt ggtctttccg cagccggatt cccccaccag cccgaacgtc tccccttgat
     7081 ggatgtcgaa gcttatgtcg tccaccgccc ggacgtgccc gatggtcgtc cggaacaccc
     7141 ctcccttgat ggggaagtgt ttgacgaggt tgcgcacgga gagcagcggg acaccgcccc
     7201 ggggcctggg cgctgtatcc ccgaccacgg caacccgcgg cgagtcttcc ccgaccccgg
     7261 tcgcgaagag tttcccttgc tcagcgtcgt acttccagca ccgtgccttg ctgccgccgt
     7321 ccagcgtcat gagcggcggc atgtgcgtcc ggcagatttc ccgcgcaaac cggcaccggg
     7381 gatgaaaggc gcacccgggg ggaggggaga gagggtccgg gatggagccg gcgatgggga
     7441 agagcttcct gcccctcagg tctgtcaggc gggggatgga ttcgaggagc gcgacggtat
     7501 aggggtggcg cggagaggtg aagatcctct tcacgtccgc gtactccact accttcccgg
     7561 cgtacatgac gacgacggtg tccgccatct cggcgatgac gcccatgtcg tgcgtgatga
     7621 tcatcaccgc ggagccgatc tggctcttga gcttgcgcat gagatccagg atctgggcct
     7681 ggatcgtgac gtcgagggcc gtcgtgggct cgtccgcgat gaggagcttg gggttgcagg
     7741 agagagccat ggcgatcatg gcgcgctggc gcatgccacc cgaaagctgg tgggggtatt
     7801 cgtggacccg gatctccggc atcgggatgc cgacgagctt cagcatgtcc acggccctgt
     7861 gcagggcggc gcgcttgccg atgcgctggt gaagcgtcag ggcctcggtg atctgcgttc
     7921 cgatggtctg cacgggattg agggaggtca tgggctcctg gaagatcatg gagatctcgt
     7981 tgccgcggat cttcctcatc tctccgggtg agctgcgcgc caggttgcgg ccgcggaaca
     8041 ggatttcgcc gtccacgatc ctgccgggcg cgtcgatcag acgcaagacg gaaagggccg
     8101 tgacgctctt cccgcacccg gactccccca ccatgcccag ggtctcgttc tcacggagct
     8161 cgaagtccac gccgtccacc gcggccacga ctcccgcctc cgtgcggaac tgtgtcttca
     8221 ggttccgaac ctcgaggagg gtgtccctca tcgcttcatc ctcgggtcca gggcgtcccg
     8281 cagaccgtca cccaggaagt tgaaggacag catcaggatg gccagggcga tgcccgggaa
     8341 gagcgccagg tgcgggtagc tccgcatggc ctggaaccca tccgcgatca tggcgcccca
     8401 gctcggcgtg ggcgggtcca ctccgagtcc gatgaagctg aggaaggcct cggtgccgat
     8461 gaaaccgggg atggccagcg tcacggagat caggagcggg ccgaggaggt ttgggaccag
     8521 ctcccggacg atgattctga ggtccccggc gccctgggcc ttggcggcct ggatgaactc
     8581 ggtttcccgc agcgagagcg ccatgccgcg ggagaggcgc gccatgccga tccacgaggt
     8641 gaggctgatg ccgacgatga tgaagagcat gccgccgaga gacttgtcca ccgccgccac
     8701 cacgcgcacg aaggggcttc ccccggtcag cgcattgccg aatcccgact tgaacgtgac
     8761 catcagcagg atgatgaaga ggaggccggg aaaggcgtac agaatgtcga cgatcctcat
     8821 gaggacgttg tcgactctcc cgccgaaata gccggagatg gttccataga gcagcccgat
     8881 ggtgaacgcc accagggcgc cgatcacccc gacgagcagc gagatccgcg tgccgtagat
     8941 cagccgactc agcatgtcgc gccccaggaa gtcggcaccc aaggggtact gcggactggg
     9001 cgcggcaaag gctgccccga agtcggattg cgcaaaggaa tgcggggcga tgagcggcgc
     9061 aaacgcgccg atgaccgcaa acgccaggat tgcgatcccc cctgcgacgg ccagtctgtt
     9121 cctgagcagg cgccggaagg catcccgcca taggcttccc ttggagcgca ttccgcgtgt
     9181 cttcctctca gcgctggctc agcttgattc gggggtcgat ccaggtgtag gtgatatcga
     9241 cgaggaggtt cgcaaccacg agcaccacgg cgtagagcag ggtggtgccc atgatgaccg
     9301 ggtagtcgcg gacacccacc gcatcgacgt agtacttgcc cagcccgggc acggcgaaga
     9361 ttcgctccac cacgagggac cccgtgagaa ggccggccgc catgggcccg accacggtga
     9421 cgacgggaat gagcgcgttg cgcaaagcgt gccgaaccat gatccgcagc tccgacacgc
     9481 ccttggcgcg ggcggtgcgg atgtagtctt cccgcagcac ctggagcatg ctcgcccgcg
     9541 tcagcctggc caggatggcg gcgcttccga tgcccagggt aaacgcggga aggatggcct
     9601 gctgcaccgt cccccatcgg gccaccggca ggattccgag cttgagcgca aagatccaga
     9661 tcaggagggg gcccatggtc atggccggta cggagacgcc ggtgagcgcg aagaacatgg
     9721 agaagtagtc cacgggcgtg ttctggcgca acgcggatac gatccccagc gggactccaa
     9781 tgaccagcgc gacgaggagc gccagcacac caagcatgat ggacacgggg aagcccccgc
     9841 ggacgatgtc gttcaccgtc cggttcaggt actggtacga ggggccgaag tcacccttga
     9901 agaggttcgc catgtagcgg aggtactgac ggtagaccgg ctcgtcgagg tggtagtagg
     9961 ccaggagctg ccggagcgtc tggggcggga ggggccgatc ccccttctga aagggattcc
    10021 ccggaatcga atgcatgagc ccgaacgtca gcaccgtcgt taccaggaga accggtatga
    10081 gccacaagag acgacggacg atatagccga tcacggcagt ccccttccga cgtatgaagt
    10141 gcccgccccg cgccggccgg gcggccggcg cgggggcggc gggtatcggg ccagcgctac
    10201 ttcctggttg tcttccaggt ccagaactcg agggtgctcg ggtcgaacgg gcgcgtgaga
    10261 tatggcttgg tcagcacatt cacggcctgg tagaacaggg gctcggccgc cacggtgtcc
    10321 tggacgtaga gcttctcgat ctgcgcgtag agctgcttcc gcctggcgag gtttggcgtg
    10381 gccgcggcat cccccaccag cttgtccaac tggtcg
//
LOCUS       DASRZS010000664         4547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2691248, whole genome shotgun sequence.
ACCESSION   DASRZS010000664 DASRZS010000000
VERSION     DASRZS010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2691248"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2076
                     /locus_tag="VFH83_16175"
     CDS             <1..2076
                     /locus_tag="VFH83_16175"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6487965.1"
                     /translation="ATDQQLEARAGNIARTIDGVFQEKLKLARLIANQPSIVGAVADV
                     AEKGSAAPRVAAATDFVTGLKQAKGVGEDYASVMVMDRNGTAIATSDARYLGVSFADR
                     DYFKAAMAGTATAGNAVLDKVTNKPVVPVVAPIRDPRTGATIGALVMGVDFTFVPNLI
                     ANEKIGKGGYAMVLDSTGIYIAHPDATRIFKENFAKMSGSEELGQTVLANDAGEGDYV
                     RDGVAKTAGYAAVPTTGWHVLFTYPRSEFMAPTDQVRNFIYVMNGAAIVVAFLVYFFF
                     SRTITRPIVRSVAFAQLVASGDFTQTMEVRRRDEVGTLVVALNGMSAKLKDMVANVQQ
                     SAQQVAAASEELSASAQSLAEGSQTQASTLEETSASVEELTASVDQVAESAQTQAAAV
                     EEGTGSMAQVQQSIDEVSSSLEEIADLAARSVENSMQGADSVGQVMEGIRRIAQSSEK
                     IGGIVAVIADIADQTNLLALNASIEAARAGEHGRGFAVVADEVSKLADRSARSAKEIE
                     ALIKEGARNVTAGVETARGSQGTMELIRAASQRVKDMIGSLTQSMQQQVAAVEEFTKA
                     LESVSEMSQSISAATEEQTTNAKQVSKAVENVNEITQSAASAAEQMSSSTEQLAGMAQ
                     ELQRMMSQFRITEGGETAAEMQAAQVAAPQVVTQQTQTGPGATAASPAANGRPHLALV
                     RQESSQAKA"
     gene            2106..4463
                     /locus_tag="VFH83_16180"
     CDS             2106..4463
                     /locus_tag="VFH83_16180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6487966.1"
                     /translation="MGRPEGGSPEPQRSREREFEALLRTRAVAVIVSHNGIMETFNDA
                     AEAMFGYPAAEAIGNPLDLLVPEPVRSEHAAHLRRYLQGDEAYLHGSVLEAQGQRRNG
                     EVFPIELILSEIPSASGRRIVACIQDLSGRRAVQGRLRTLSSAVEQSTSTIVITDREG
                     RITYVNPAFTRVTGYTFEEARQGTPRILKSGLHPAELYDGLWKTISSGSPWRGMLCNR
                     KKSGELYWEAASISPVRDDTGAITHYVGVKDDVTENKRQEQRLRDLALVAEETENVVV
                     ITDAGGRVEWVNPSFTRVTGFAGVEVVGKDYQGFLRDPDGDPSAPRWTPGTSDAARAF
                     RAELLHVTRSGDPVWLDANIQPITGDRGEITKYISVASDVTDRRKVTEELRRAKEAAD
                     AASRTKSEFLANMTHEIRTPLNVVLGFASIALTADPDPRLRDYLTKIRVAGESLLRVV
                     DDVLDFSKIEAGKLRTERVAFSLGPVVDTVASLITPKAYGKGLQFLFLQPDADLRLVG
                     DPTRLGQVLINLLDNAVKFTAEGEVELHVEVESRAQDRIGLRFTVRDTGIGMTGEEMS
                     MLFQPFTQADSSMKRRFGGTGLGLSITKRLVEMMDGRIAVQSEPSKGTTFTFTAWFGS
                     DGERSAAAASPTVGARDTAASEAASGFEYRAILAGARILLVEDNEVNAEVASTLLRSA
                     GVVVYTATNGRAAVERAALATPPFDLILMDIQMPGMDGYEAAQLIRSMTRLADVPIVA
                     LTAHAMVQQRERALAGGMDDHIGKPIDPRVLFETVARNLKGRRAT"
BASE COUNT          821 a         1491 c         1589 g          646 t
ORIGIN      
        1 gccaccgacc agcagctcga ggcccgtgcg ggcaatatcg cgcggaccat cgacggcgtc
       61 ttccaggaga aactgaagct cgcgaggctg atcgccaacc agccctcgat cgtcggggcg
      121 gtcgccgatg tcgcggagaa gggatctgcc gcccccaggg ttgccgcggc aacggacttc
      181 gtcacgggcc tgaagcaggc caagggtgtg ggagaggact acgcctccgt gatggtcatg
      241 gaccggaacg ggaccgcaat cgccacttcc gacgccaggt accttggggt gtcctttgcg
      301 gatcgtgact acttcaaggc cgccatggcg ggcaccgcga ccgcgggcaa cgccgtgctc
      361 gacaaggtga ccaacaagcc ggtggtcccg gtggtggcac ccattcgaga cccgcggacc
      421 ggcgccacca tcggagcgct ggtcatggga gtggacttca ccttcgtgcc gaacctgatc
      481 gccaacgaga agatcgggaa gggcgggtat gcgatggtgc tggacagcac cggcatctac
      541 atcgcgcatc ccgatgcaac gcggatcttc aaggagaact tcgccaagat gagcgggtcc
      601 gaggagctcg gccagaccgt cctcgccaac gacgcgggcg aaggggacta tgtgcgggat
      661 ggcgtggcca agaccgccgg ctacgccgcg gtccccacca cgggatggca cgtgctgttc
      721 acgtatcccc gaagcgagtt catggcgccc accgaccagg tccgcaactt catctacgtg
      781 atgaacggcg cggccatcgt cgtggcattc ctcgtctact tcttcttctc ccgaaccatc
      841 acgaggccga tcgtcaggag cgtggccttt gcccagctcg tggcttctgg ggacttcacc
      901 cagacgatgg aggttcgtcg ccgcgacgag gtcgggaccc tggtcgtcgc tctcaacgga
      961 atgtcggcga agctgaagga catggtcgcc aacgtccagc agagcgcgca gcaggtggcg
     1021 gcggccagtg aggagctttc cgccagcgcc cagagcctgg cggaggggtc gcagacccag
     1081 gcgtccacgc tcgaagagac aagcgcctcc gtcgaggagc tgaccgcctc ggtggaccag
     1141 gtggccgaga gcgcgcagac ccaggccgcg gcggtagaag aggggaccgg ctccatggcc
     1201 caggtgcagc agtccatcga cgaggtctcc agcagcctgg aggagatcgc cgatctggcg
     1261 gcgcggtccg tggagaactc catgcaaggc gcggactcgg tggggcaggt gatggaaggg
     1321 atccgccgga tcgcgcagag ctcggagaag atcggcggga tcgtggccgt gatcgcggac
     1381 attgccgacc agacgaacct cctggcgctg aacgcatcga tcgaggccgc ccgcgccggc
     1441 gagcacggac gcggattcgc cgtggtggcc gacgaggtca gcaagctcgc cgaccgaagc
     1501 gcccggtccg ccaaggagat cgaggccctc atcaaggaag gcgcccggaa cgtaaccgcc
     1561 ggcgttgaga ccgcccgagg ctcccagggc accatggagc tgatccgcgc cgcctcccag
     1621 cgggtcaagg acatgatcgg ttccctgacg caatccatgc agcagcaggt ggcggccgtg
     1681 gaggagttca ccaaagccct ggagagcgta agcgagatga gccagagcat ctccgcggcc
     1741 acggaggagc agaccacgaa cgccaaacag gtgtccaagg cggtggagaa cgtgaacgag
     1801 atcacccagt ccgccgcgtc cgccgccgag cagatgtcct cttccacgga gcagctcgcg
     1861 gggatggcgc aggagcttca gcggatgatg tcccagttcc gcatcaccga agggggggag
     1921 acggccgcgg aaatgcaggc tgctcaggtc gcggctcccc aggtcgtgac ccaacagacg
     1981 cagacgggcc cgggcgccac cgccgccagc ccggccgcca acggccgtcc ccacctggcg
     2041 ctggtacgcc aggagtcttc ccaggcgaag gcctgagggc acgggaggaa gacgccgtga
     2101 gcgagatggg tcgcccggag gggggctccc cggagcccca gcggtccagg gaacgggagt
     2161 tcgaggcgct tctgaggacg cgggccgtcg ccgtgatcgt ctcccacaac ggcatcatgg
     2221 agacgttcaa cgacgccgcg gaggccatgt tcgggtaccc ggcggcggag gccatcggca
     2281 acccgctcga tctcctggtg ccggagccgg tccgctcgga gcacgcggcg cacctgcggc
     2341 gctacctcca gggcgacgag gcctatctcc acgggagcgt cctggaggcc caaggtcagc
     2401 ggcgcaacgg agaggtgttc ccgatcgagc tcatcctgag cgagatcccg agcgccagcg
     2461 ggcggcgcat cgtggcctgc atccaggacc tctcggggcg tcgagcggtc cagggcaggc
     2521 tgcggaccct ctcgagcgcc gtcgagcaga gcacctcgac catcgtcatc acggatcgcg
     2581 aaggccggat cacctacgtg aacccggcct tcacccgggt caccggctat accttcgagg
     2641 aggcccgcca gggaacaccg cggatcctga aatccgggct ccatcccgcc gagctctacg
     2701 acggactgtg gaagaccatt tcctccggca gcccctggcg cgggatgctc tgtaaccgga
     2761 agaaaagcgg ggagctgtac tgggaagccg cctcgatctc gccggttcgg gacgacaccg
     2821 gcgccatcac gcactacgtg ggcgtcaagg acgatgtaac ggagaacaag cgccaggagc
     2881 aacggctgcg cgatctggcg ctcgttgccg aggagaccga gaacgtcgtc gtcattaccg
     2941 acgccggcgg ccgggtggaa tgggtcaatc catccttcac tcgggtgacc ggtttcgccg
     3001 gcgtggaggt cgttgggaag gattaccagg ggttcctgcg ggaccccgat ggtgaccctt
     3061 ccgcgccccg gtggactcct gggacctcgg acgcggcccg tgcgtttcgg gcggagctcc
     3121 tccacgtcac ccggtcgggg gatccggtct ggctggacgc gaacatccag ccgatcaccg
     3181 gcgatcgggg cgagatcacc aagtacatct ccgtcgcctc cgacgtcacg gaccgtcgaa
     3241 aggtcaccga ggagctgcgc cgggccaaag aggccgcgga tgcggccagc cgcacgaaga
     3301 gcgagttcct ggcgaacatg acccacgaga tccgcacgcc gctcaacgta gtcctggggt
     3361 tcgcttccat cgcgctcacc gcggatccgg acccgaggct gcgcgactat ctcacgaaga
     3421 tccgggtcgc cggcgagagc ctgctgcggg tggtcgacga cgtcctggac ttctccaaga
     3481 tcgaggccgg gaagctgcgc accgagcggg tagccttctc cctgggtccg gtggtcgaca
     3541 cggtggcttc cctgatcacg cccaaggcct acgggaaggg gctccagttc ctgttcctgc
     3601 agccggatgc cgacctgcgc ctggtcgggg accccacccg gctgggccag gtgctcatca
     3661 acctcctgga caacgccgtg aagttcaccg cggaagggga ggtcgagctc cacgtggagg
     3721 tggagagtcg ggctcaggac cggatcggtc tgcggttcac cgttcgggac acgggcatcg
     3781 ggatgacggg ggaagagatg tccatgctct tccagccgtt cacccaggcg gacagctcga
     3841 tgaagcgccg attcggcggg acagggctcg ggctgagcat caccaagcgg ctggtggaaa
     3901 tgatggatgg ccgcatcgcc gtgcagagcg agccgtccaa gggaacgacc tttacgttca
     3961 ctgcctggtt cggctccgac ggggagcgct cggcagcagc ggcgtcccca actgtggggg
     4021 cgagggacac cgcggcgagc gaagcggcgt cgggtttcga ataccgcgcc atcctggcgg
     4081 gcgcccggat cctcctggtg gaggacaacg aggtcaacgc ggaggtggct tcgacgctgc
     4141 tgcgaagcgc cggcgtggtg gtctacacgg ccaccaacgg ccgcgcggcg gtggagcggg
     4201 cggcgctggc cacgcctccg ttcgacctca tcctgatgga tatccagatg cccgggatgg
     4261 acggctacga ggctgcccag ctcatccgct ccatgacccg gcttgccgac gtccccatcg
     4321 tcgcgttgac cgcgcacgcc atggtgcagc agcgcgagcg ggccctggcc ggcgggatgg
     4381 acgaccacat tggcaagcct atcgatccgc gggtgctgtt cgagacggta gcccgcaacc
     4441 tcaagggtcg gcgcgcaacc tgatggttcc gagcgacccg cgccgaggga cagcgtccag
     4501 gccaccgcgc cgggcaactg cgccggacag cgcggcgccc gcgtagc
//
LOCUS       DASRZS010000665         6853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4594774, whole genome shotgun sequence.
ACCESSION   DASRZS010000665 DASRZS010000000
VERSION     DASRZS010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4594774"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..987)
                     /locus_tag="VFH83_16185"
     CDS             complement(13..987)
                     /locus_tag="VFH83_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487967.1"
                     /translation="MAVAVAAALPAQSPDLTTFTDTFQGFTTAVAGTLASTATIGLNW
                     DQAYIGQFPHLGLGVTVGASLIPVTAVQKITDMMGVTLPAQLQAYGVPIPAATLDARI
                     GGFFLPFDIGLEFGTIPDAAKNLLGGIQLNYMMAGGDIRFALLKDHGFTPALSVGAGY
                     AFFQGSIGVPGLFPGASVDITQLMNAAGYGGTNTLTFSDPDLQFNWQSNVIEAKVQLS
                     KTLLFITPSIGFSAAYGISTAGGGLSSTISYSGSATTAQMQSVLQAAGLPVPTDQSLS
                     VSSSSNGWAFRAFGGINIALLFLNLDANAFYNVVNGSYGGSVSLRFQI"
     gene            1156..2574
                     /locus_tag="VFH83_16190"
     CDS             1156..2574
                     /locus_tag="VFH83_16190"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6487968.1"
                     /translation="MNLASRALVFGVLFLGAAPLGAQALPDAAWVGSWLGELKAGAVT
                     LRIVFTIASTDGALSATMDSPDQGAKGIPVSAVTVTADAIRLEVKVANAWYAGTLTTD
                     GKSIDGTWNQGGAVFPVLLSRLPGAFTLERPQDPKPPFPYTAQDVTFPNQKAGVQLAG
                     TLTIPAGKGRFPAVVLVTGSGPQNRDEAIMGHKPFAVIADFLSRNGIAVLRYDDRGVA
                     GSKGSFAGSTTLDFADDAEAAFGYLAARPEVDPKRVGILGHSEGGLIAPIVAARNPAV
                     GFIVLLAGPGVRGDQLILAQGDALARAAGTDAATADWSHALNAKLYQIIEQPGDPAAN
                     AAEAKDTLLAGIDSAPKLTDQERQTAKAQADNSIAQLSSAWFRLFLSLDPATYLSHVT
                     VPVLALNGTLDLQVPPDLDLPAIQAALTTAGNKHYTLMKLDGLNHLFQHATTGLPDEY
                     GKLTETFAPEALSAIRDWILAR"
     gene            2640..2918
                     /locus_tag="VFH83_16195"
     CDS             2640..2918
                     /locus_tag="VFH83_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487969.1"
                     /translation="METCKNYQKCPIYSGLLADIKMASAAYRKYYCDAGEKGWTSCRR
                     FQVKEKGGKVPENLLPNSHMSVEEVIKRHGMATPSAAKPAAAPSPAGG"
     gene            complement(3000..4250)
                     /locus_tag="VFH83_16200"
     CDS             complement(3000..4250)
                     /locus_tag="VFH83_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite family transporter"
                     /protein_id="HET6487970.1"
                     /translation="MDGKRMLVVDDEEVVLESCRRVFTHEGFEVSTTKSARDALERVA
                     ATRFDVILCDWKMPEMDGVDVVDLMDKRAPETPIVMISGYPSVERATEVMKRGAMDYV
                     AKPFTPEEIIGAVNRAMGRKVQQEKGRVERLVKSFPVPSVDDASPQTIADTVARKVGV
                     GKATSPWVSVLVLGILAGAYIAFGGMLASTVTFDLASRAGTGIARLVSGSVFSLGLML
                     VVIAGAELFTGNNLMISSAMSGSIGLGRMLERWGLVYVANFVGSMLLALLFFYSGLWK
                     TSDGALGAYAVKVAYTKVNLSFVEALMRGIGCNWLVCLAVWMALASRQVIGKIFAIFF
                     PIMGFVALGFEHSVANMYFIPTGILLHTLGGLPATDGFDPSSLGWGSFVVRNLLPVTV
                     GNIIGGGLFVGMGYWGAYLSRNKA"
     gene            complement(4316..4684)
                     /locus_tag="VFH83_16205"
     CDS             complement(4316..4684)
                     /locus_tag="VFH83_16205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6487971.1"
                     /translation="MNVLVVDDDPIVIASCAKVLVFDGLGVRTARSVPDAIQELGRER
                     YDLLLVDLKMPEYDGLHLMRIAREDHPDLPILVMSGFPTPETVARCTEAGASGFVPKP
                     FTPRELLDAVQRAAGRTGRQ"
     gene            complement(4681..6651)
                     /locus_tag="VFH83_16210"
     CDS             complement(4681..6651)
                     /locus_tag="VFH83_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HET6487972.1"
                     /translation="MIISIRVRLIASFLAVTVLVGGLSLLVGGQLIYRAVLREAQTRI
                     DQDLGAAREIYDERERSLLQGLVLAAGEEGVRSDVRAGAEPALDARLQDVKAALGLDF
                     IGVVAPDGRRLLTDAVPRSTPPAGAASPIASLALQRGAAVSGTLVLSREELLAESPAL
                     ADRARIALIPTARAAPRDDAPETSGLALCAAVPLTERGIRLGALYGGVLLSRNQDIVD
                     RIRDTVFREEAFGGRLIGTATIFYRDLRIATNVQTAAGDRAVGTRVSAEVRQRVLDQG
                     ERWSGRAFVVNDWYVTAYEPIVDIAGQRVGMLYVGVLESKYASVRISALLVFVVITML
                     GMAAAVALGALLSYRILRPVQELVEASRRVSEGDLGAKIWPSSRGEMGVLQRTFSEML
                     LALQERDQRLLADRERQLVQSEKQASIGRLAAGIAHEINNPLTGVLTFTHMLLARTDL
                     DASAREDLGVIAKATERVRTIVKGLLDFSRQTAIEPKPTDVNDLVVQTMRLAANQALI
                     KGVKFCFNPTEGLPPRTLDRNQMQSVLLNLLINSIDATEGGGHIDVSTAFRPAGEGKG
                     PGGIEIRVSDTGCGIPAENLDRIFDPFFTTKEVGKGTGLGLSVSQGIVERHGGTIQVS
                     SRPGEGATFVVSLPLDGGVPRPGDWPTAEARR"
     gene            6732..>6853
                     /locus_tag="VFH83_16215"
     CDS             6732..>6853
                     /locus_tag="VFH83_16215"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET6487973.1"
                     /translation="MDAIILAGGSAVRLGGANKALLQVGGERFIDRILRTLAPL"
BASE COUNT         1211 a         2314 c         2236 g         1092 t
ORIGIN      
        1 gccgacggga cgctatatct ggaaccggag gctcacgctg ccgccgtagg agccgttgac
       61 cacgttgtag aacgcgttgg cgtccaggtt gaggaacagc agggcgatgt tgatgccgcc
      121 aaaagcgcgg aaggcccagc cgttggagga agaagacacg ctcagcgact ggtccgtggg
      181 aacgggcagg ccggcggctt gaagcacgct ctgcatctgc gccgtcgtcg cgcttcccga
      241 gtagctgatg gtggacgaaa gcccaccgcc cgccgtggag atgccgtagg cggcgctgaa
      301 accgatgctc ggcgtgatga agaggagcgt cttgctgagc tgcactttgg cttcgatcac
      361 gttgctctgc cagttgaact gcaggtcggg atcgctgaac gtcagcgtgt tcgttccgcc
      421 gtatcccgcc gcgttcatga gctgcgtgat atccacgctc gcccccggga acagacccgg
      481 cactccgatg gatccctgga agaaggcgta gccagccccc acggagagcg ccggcgtaaa
      541 tccatggtcc ttgaggagcg cgaaccggat gtctcctccc gccatcatat agttgagctg
      601 gatgcctccc aggaggttct tcgccgcgtc ggggatcgtg ccaaactcca ggccgatgtc
      661 gaacggcagg aagaatccgc cgatccgcgc gtccagggtc gccgcgggaa tgggcacgcc
      721 gtaagcctga agctgagccg ggagcgtgac ccccatcatg tcggtgatct tctggaccgc
      781 agtgaccgga atgaggctgg cacccaccgt gaccccgagg cccagatgcg ggaactgccc
      841 gatgtaggct tgatcccagt tcagaccgat ggtggccgtg gaggcaagcg ttccggctac
      901 cgcggtggtg aaaccctgga acgtatccgt gaacgtcgta aggtccgggc tttgggcggg
      961 gagcgccgcc gccacagcaa cggccattat cacaaccaag agagtttttc tcatcactac
     1021 tatccctccg tgccataaac agtagcggtc gggagggcga atttcaaacg aaggcctggc
     1081 cttccgcgca tggagccagg cgcccttttc tcgatctctc ccgcatgtca gaatgtcccc
     1141 acacgaggag agaccatgaa ccttgcatcc cgcgcgttgg ttttcggtgt gctgttcctg
     1201 ggcgccgccc ctctgggagc ccaggcgctg ccggacgctg cctgggtggg gtcctggctc
     1261 ggggagctga aggccggggc ggtcaccctt cgcatcgtat tcaccatcgc gtccaccgac
     1321 ggcgccctgt cggcgacgat ggacagtccg gatcagggag cgaaaggcat tcccgtttcc
     1381 gccgtgaccg tgacggcgga tgccatccga ctggaggtga aggtggcgaa cgcgtggtac
     1441 gccggcacgc tgaccacgga cggcaagagc atcgatggga cctggaacca gggcggggcg
     1501 gtgtttcccg tcctcctctc gcggctcccc ggcgccttca cgctggagcg tccccaggac
     1561 cccaagccgc cgtttccgta cacggcacag gacgtgacgt tcccgaacca gaaggccgga
     1621 gtccagctcg ccgggacgct cacgatcccg gccggcaagg gacgcttccc cgcggtcgtg
     1681 ctggtcacgg gatccggacc gcagaaccgc gatgaagcga tcatgggtca caagccgttt
     1741 gccgtcatcg ccgacttcct ttcgaggaac ggaatcgccg tcctgcgata cgacgaccgc
     1801 ggcgtagcgg ggtccaaggg cagcttcgcc ggatccacca cgctggactt cgcggacgat
     1861 gccgaggcag ccttcggcta cctggcggcg cgacccgagg tggacccgaa gcgggtcggc
     1921 atcctggggc acagcgaagg aggcctgatc gcccccatcg tggcggcaag gaatccggcg
     1981 gtgggcttca tcgtcctcct ggcgggtccc ggagtgcgcg gggaccagtt gatcctggct
     2041 cagggggatg cgctggccag ggcggctggg acggacgccg ccacggccga ctggtctcat
     2101 gccctcaacg caaagctcta ccagatcatc gagcagccgg gagatcccgc ggcgaacgcc
     2161 gccgaggcca aggacacgct cctggcaggg atcgattcgg cacccaagct cacggaccag
     2221 gagcgacaga ccgccaaggc gcaggcggac aactccatcg cgcagctttc ctccgcctgg
     2281 tttcggctct tcctgtcgct ggacccggcg acgtatctct ctcacgtcac cgtcccggtg
     2341 cttgcgctga acgggaccct ggatctccag gtgccgcccg atctcgatct tcctgccatc
     2401 caggcggccc tgacgacggc cggcaacaag cactatacgc tcatgaagct cgacggcctg
     2461 aatcacctct tccagcacgc cacgacgggg ctgccggatg agtacggcaa gcttaccgag
     2521 acgttcgcgc cggaggcgct ctcggccatc cgcgactgga tccttgcccg ctgacgaaag
     2581 ggaacggtgg cgagctccga agcgagatag actttcttac aaatcggagg ttggcgggaa
     2641 tggagacgtg caagaactac cagaagtgcc cgatctacag tgggcttctg gccgacatca
     2701 agatggcctc agcggcctat cggaagtact actgcgatgc gggggagaag ggctggactt
     2761 cctgcaggcg cttccaggtc aaggagaagg ggggcaaggt cccggagaac ctgttgccca
     2821 actcccacat gagcgtggaa gaggtcatca agcgccacgg catggcgacc ccgtcggccg
     2881 ccaagcctgc cgcagcgcca tccccggcgg gcggatagcg ccggcccagc gggtcgcgcg
     2941 ttcgccgggc tcggggccct gggcgcgccg gtcaggacgc gcgccccggc tccatcctcc
     3001 tatgccttgt tcctgctcag atacgctccc cagtatccca tcccgacgaa cagcccaccg
     3061 ccgatgatgt tgccgacggt gaccggcaga aggttccgca ccacgaagga gccccacccc
     3121 agggagctgg gatcgaagcc gtcggtggcc ggcaacccgc cgagggtgtg aaggaggatc
     3181 ccggtgggaa tgaagtacat gtttgccacc gagtgctcga accccagggc aacgaatccc
     3241 atgatgggga agaagatcgc gaagatcttc ccgatgacct gccgcgaggc gagcgccatc
     3301 cagaccgcga gacagacaag ccagttgcag ccgatccccc gcatcagcgc ctcgacgaag
     3361 gacaggttga ccttcgtata ggccaccttg accgcgtagg cgcccagcgc gccgtcggag
     3421 gtcttccaca ggccggagta gaagaacagc agggccagga gcatggagcc gacgaagttg
     3481 gcgacgtaca ccagccccca acgctccagc atccgcccga gcccgatgct tcccgacatc
     3541 gcgctggaga tcatcaggtt gtttcccgtg aagagctcgg cgcccgcgat gacgacgagc
     3601 atcagcccga ggctgaacac ggatccgctc accagcctgg cgatcccggt cccggcgcgg
     3661 gaggccagat cgaaggtgac cgtggaggcc agcatgccgc cgaacgcgat gtaggcgccg
     3721 gcgaggattc ccaggaccag cacggacacc cacggcgagg tggccttgcc gacccccacc
     3781 ttgcgggcaa cggtatccgc aatggtctgc ggcgaggcgt catccacgga cggaaccgga
     3841 aacgatttca ccaaccgttc cacccggcct ttctcctgct ggaccttgcg gcccatggcc
     3901 cgattcaccg cgccgatgat ctcttccggc gtgaacggct tggccacgta gtccatggcg
     3961 ccccgcttca tgacctccgt ggcccgctct accgaggggt agccgctgat catgacgatc
     4021 ggcgtctccg gggcccgctt gtccatcagg tcgacgacgt ccaccccgtc catctcgggc
     4081 atcttccagt cgcacaggat cacgtcgaag cgcgtcgcgg cgacccgctc cagcgcgtcc
     4141 cgagcgctct tcgtcgtcga gacctcgaac ccctcgtgcg tgaagaccct ccgacaggac
     4201 tccagcacga cctcctcgtc gtccaccacg agcatccgct tgccatccat gcctgtcctc
     4261 ctgctgctcc gtgcgaaccg gctccgtgcc aatccgtagg cgatgagacc gaccgctact
     4321 gcctacccgt ccggcccgcg gcgcgctgga cggcgtccag cagctcccgg ggagtgaacg
     4381 gcttgggaac aaagccggac gctcccgcct ccgtgcatcg cgccacggtc tccggcgtcg
     4441 ggaacccgct catgacgagt atgggaaggt cgggatggtc ctcccgcgcg atccgcatca
     4501 gatggaggcc atcatactcg ggcatcttca gatccacaag cagaaggtcg taccgctcgc
     4561 ggccgagctc ctggatggca tccggtacgc ttcgcgcggt ccgcacgcca agaccatcga
     4621 agaccaggac cttggcgcag ctggcgatga cgatgggatc gtcatccacc accaggacgt
     4681 tcaccggcgg gcctcggcgg tgggccagtc cccgggacgc ggtaccccgc catcgagagg
     4741 caggctcacc acgaaggtgg cgccctcccc cggcctgctg cttacctgga tcgtgccgcc
     4801 gtggcgctcc acgataccct gggagaccga gagtcccagg cccgtcccct tccccacttc
     4861 cttggtggtg aagaaaggat cgaagatccg gtccaggttc tcagcgggaa tcccgcaccc
     4921 cgtgtcggag acccggatct cgatgccgcc cggtcctttg ccctcccccg ccgggcgaaa
     4981 cgcggtgctc acgtcgatat gccctccccc ctcggttgca tcgatggagt tgatcaggag
     5041 gttgaggaga acactctgca tctggttacg gtccagggtc cggggcggaa ggccctccgt
     5101 ggggttgaag cagaacttca cgcccttgat cagggcctgg ttggcggcca gccgcatggt
     5161 ctggaccacc aggtcgttga cgtccgtagg cttcggctcg atggccgtct ggcgggagaa
     5221 gtcgagcagg cccttcacga tcgtccgtac ccgctcggtg gccttcgcaa tgaccccgag
     5281 atcctcacgc gcggaagcgt ccaggtccgt cctggcaagc agcatgtgcg taaaggtgag
     5341 cacgccggtc aacgggttgt tgatctcgtg agcgataccg gccgccaggc ggccgatgct
     5401 cgcctgcttc tcggattgaa cgagctgccg ctcacggtct gccaggagcc gctggtcgcg
     5461 ctcctggagc gccaggagca tctcgctgaa ggtcctctgc aggaccccca tctctccgcg
     5521 ggaggagggc cagatctttg cccccaggtc tccctccgag acccgccggc tcgcctcgac
     5581 gagctcctgc accggcctga ggatccgata gctcagaagc gcgccaaggg cgaccgctgc
     5641 cgccattccg agcatcgtga tgacgacaaa gaccaaaaga gcgctgattc ggacggacgc
     5701 gtacttggac tccagcaccc cgacgtagag catccctact cgctgcccgg cgatatcgac
     5761 gatgggctcg taggcggtga cgtaccagtc gttgaccacg aaggcccggc cgctccagcg
     5821 ctctccctgg tccaggactc tctgacggac ctcggcggag acgcgcgtcc ccaccgcccg
     5881 atctcccgcc gccgtctgga cgttggtagc gatccgcagg tccctgtaga agatcgtggc
     5941 cgtcccgata agccgtccgc cgaaggcttc ctcacggaac acggtgtccc ggatccggtc
     6001 cacaatgtcc tggttgcggc tgaggagcac gccaccgtag agagcgccga gacggatccc
     6061 ccgttcggtc aggggaaccg ccgcgcagag cgccaggccg gacgtctccg gggcgtcgtc
     6121 gcgcggcgcc gcgcgggcag tcgggatgag cgctattcgg gcacgatcgg ccagcgcggg
     6181 ggattccgcc agaagctcct cccggctgag gacgagcgtc ccggaaacgg cggcgccgcg
     6241 ctgaagggcc agggaggcga tagggctggc ggcgcccgcc ggcggggtgg accgcggcac
     6301 ggcatcggtc aggagcctgc gcccgtccgg ggccacgacg ccgatgaaat cgaggcccag
     6361 ggcggccttc acgtcctgga gccgcgcgtc aagcgctggc tcggcgccgg cccgcacgtc
     6421 ggaccgcacc ccctcttccc ccgccgcaag cacgaggccc tggaggagcg accgctcgcg
     6481 ctcgtcgtag atctcgcggg cggcgcccag gtcctggtcg atgcgggttt gggcttcccg
     6541 caggacggcg cggtagatga gctggccgcc caccagaagg gagagaccgc ccaccagcac
     6601 ggttacggca aggaagctgg cgataaggcg cacgcggatc gagatgatca ccgggggcta
     6661 cgataggagc cactcccggg gctgtcaagg gaatcggtcc cgggtatagt agccgcgctt
     6721 tgggtggatc ggtggatgcg atcattcttg ccggcggaag cgcggtacgg ctgggaggcg
     6781 ccaacaaggc ccttctgcaa gtgggtgggg agcggttcat cgaccgcatc cttcgcacgc
     6841 ttgccccgct ctt
//
LOCUS       DASRZS010000666         3501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1920225, whole genome shotgun sequence.
ACCESSION   DASRZS010000666 DASRZS010000000
VERSION     DASRZS010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920225"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFH83_16220"
     CDS             complement(<1..450)
                     /locus_tag="VFH83_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase c2"
                     /protein_id="HET6487974.1"
                     /translation="MKADRRLTPEAIDQIRAAVEEAGGNEVFLVGTVGDDGRVASVRV
                     GARGNEEAVPVLSPHLEDGGCVIHNHPSGGTRPSNADLSIASRLGNQGIGFYIVDNTV
                     EEVYVVAEPVAAREIVLLDAGQLADAISPGGALSRLYPLYENRESQVE"
     gene            complement(440..1270)
                     /locus_tag="VFH83_16225"
     CDS             complement(440..1270)
                     /locus_tag="VFH83_16225"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET6487975.1"
                     /translation="MERQFGATLDAAWASLSEHHERKPSIGVILGSGLGAMAASASGP
                     KVLFESIPGFPRPTVAGHAGFIALGRTVAIQAGRYHYYEGHPMDRVVLPVFLLHRFGV
                     RTLIVTNAAGGIDRAYHPGDLVLLRDHVNLMGANPLRGPEHPAGPRFPDLSEAYDRGL
                     RETAQQAARAAGQPPLAEGVYAALAGPSYETPAEIRMLAAIGADLVGMSTVPEVIAAR
                     FLGMRVLGISCVTNMAAGILPQPLDHAEVMARGREAGPRFIALIQGILERLGGSTGEG
                     "
     gene            complement(1284..2252)
                     /locus_tag="VFH83_16230"
     CDS             complement(1284..2252)
                     /locus_tag="VFH83_16230"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HET6487976.1"
                     /translation="MNITIVGPGAVGTLLGGLLQLSGVSVTLRGRRAVPAPSEPLRIL
                     LPGGWLLAEGLRRESPEDPVGSPDLFLVTLARHHLHSVRRADFLRLIGDSAPVAFCNA
                     DPVEAERLAVPSVRLFRFCTLWNAVKLQESEVELTTEEPAIVVERQPVLLGAVQGLAS
                     RGIRVIPVDDVGPFVNSLFLYQLLFLPVAMCNTTLEAFLSVPEGRELALRVLQEGFSA
                     ADRAGLAVSRLPAQDPWDLASRLEKKPSGFDAERHVPGRAYNSVLQSYLLDRPSEAAQ
                     LNRRIVEIASEAGLHLTWNWRLFQKASRIGSVGYFRDPAELLRALV"
     gene            2368..>3501
                     /locus_tag="VFH83_16235"
     CDS             2368..>3501
                     /locus_tag="VFH83_16235"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET6487977.1"
                     /translation="MVVMKFGGTSVQNAEMMDATLTIAENQIDRAPVLVSSAMSKVTD
                     QLQEIAKLVGAGKEAEACGLLGAIEERHLACARSFLTGATLSRCEADLRAVCGELGAL
                     IKALSLLKEWSKRSNDAILSFGERLSTIVLLHRARERGIDAELHDSRELVKTDDSFTA
                     AAPLEDLTNRLVGSKVRARPGHLAILQGFIASTVTGAITTLGRGGSDYTATIVGAALR
                     AEEVQIWTDVTGIMTSDPRIVPSARTIDQISYREAAELAYFGAKVIHPSTIQPAVNLA
                     IPVWVKNTFRPQDAGTRISASIPGSGMRAIASKKGITVVSVSSSRMLLAYGFLRRIFE
                     IFEKHETPVDLIATSEVSVSVTIDTPTAVDAIARDLAEIGTVGV"
BASE COUNT          608 a         1169 c         1189 g          535 t
ORIGIN      
        1 ctccacctgg ctctcgcgat tctcgtagag gggatacagg cggctcagag cgccgcccgg
       61 agaaatggca tccgcgagct ggcccgcatc caggaggacg atctctcggg cggcgacggg
      121 ctccgcgacg acgtagacct cctctaccgt gttgtccacg atgtagaagc cgatcccctg
      181 gtttcccagc cgggaggcaa tcgagaggtc cgcgttgctg ggccgggtgc cgccggaagg
      241 atggttgtgg atgacgcagc cgccgtcctc caggtgcgga gaaaggaccg ggacggcctc
      301 ctcgttgccg cgcgcgccca cgcgcacgga agccacccgc ccgtcgtcac cgacggtgcc
      361 tacgaggaac acctcgttgc ccccggcctc ctcaacggcg gcgcggatct ggtcgatggc
      421 ctccggcgtg agccggcggt cagccttcac ccgtggaacc ccccagccgc tcgagaatcc
      481 cctggatcag cgcgatgaac ctgggtcccg cctccctgcc tcgggccatg acctcggcat
      541 ggtccagcgg ctggggcagg atgccggcgg ccatgttcgt gacgcaagag atcccgagca
      601 cacgcatccc caggaaccgc gcggcaatca cctcgggaac cgtcgacatc ccgaccaggt
      661 ctgcgccgat ggcggcgagc attcggatct cggctggggt ttcgtacgac ggtcccgcga
      721 gcgccgcgta gacgccctca gcaagaggcg gctgcccggc ggctcgggcg gcctgctggg
      781 cggtctcgcg caggccccgg tcgtacgctt cgctcaggtc cgggaaacgc ggcccggccg
      841 ggtgctccgg accgcgcagc gggtttgcgc ccatcaggtt cacgtggtcc cgcagaagga
      901 ccaggtcccc cggatgatac gctcggtcga tccccccggc cgcgttggtg acgatcaggg
      961 tacgaacgcc gaagcgatgg aggaggaaca cgggcagcac cacgcggtcc atggggtgcc
     1021 cctcgtagta atggtaccgc ccggcctgga tggcgacggt ccggcccaat gcgatgaagc
     1081 ccgcgtggcc tgccacggta ggcctgggga atcccgggat ggattcgaag aggaccttcg
     1141 gcccgctcgc gctcgcggcc atggccccca gcccggaacc caggatcacc ccgatggacg
     1201 gctttctttc gtggtgctcc gagagtgacg cccaggctgc gtcgagggtt gcgccgaact
     1261 gtcgttccac ggtgtcctcc ggtctatacc agcgcgcgaa gcagctccgc gggatcgcgg
     1321 aagtagccga cgctcccgat ccggctggcc ttctgaaaca gccgccagtt ccaggtgagg
     1381 tgcagacccg cctccgaggc gatctcgacg atccgacggt tgagctgcgc cgcctcggac
     1441 ggccggtcca gcagatagga ttgcaggacg ctgttgtatg cgcgccccgg aacatgccgc
     1501 tccgcgtcga accccgacgg cttcttctcc aggcgggagg caagatccca gggatcctgg
     1561 gcgggaagtc gggacacggc aagaccggcg cgatcggcgg cggagaagcc ctcctgaagc
     1621 acgcggagcg ccagctcacg accctccggt accgagagga aggcctccag ggtcgtgttg
     1681 cacatggcta cgggcaggaa gaggagctgg tacaggaaga gcgaattgac gaacggacca
     1741 acgtcgtcca cggggatcac gcggatgcct cgggacgcca gaccctggac agcgccgaga
     1801 agcacgggtt ggcgttcgac gacgatggcc ggctcctccg tggtcagctc gacctcgctc
     1861 tcctggagct tcacggcgtt ccacaacgtg cagaaccgaa agagtcgaac ggatgggacg
     1921 gccagccgtt ccgcctccac cgggtccgcg ttgcagaagg cgacgggtgc cgagtctccg
     1981 atgagcctca ggaagtccgc gcgcctcacg gagtgcaggt ggtgtcgggc caacgtcacc
     2041 aggaacagat ccgggcttcc cacggggtcc tcggggcttt cccgccgaag cccttcggcg
     2101 agcaaccaac cgccgggcag gaggatgcgc agcggctcag agggagcggg caccgcccgc
     2161 cgacccctca gcgtcacgga aacgccagag agctgcagaa gcccgcccag gagagtcccg
     2221 accgcgccgg gcccgacaat cgtgatgttc atcgtgtccg aaccggatta tccagggtcg
     2281 gatgaaaatg gcaagggggc ggagatttat gcgcgatccg catgaatatg attgacatgc
     2341 ccggtccctc catgctacct cctgcgcatg gtcgtaatga agttcggcgg cacctcggtg
     2401 cagaacgcgg agatgatgga cgcgacgctc acgatcgctg agaaccagat cgatcgcgcc
     2461 cccgtgctgg tgagctctgc catgtccaag gtcaccgacc agcttcagga gatcgccaag
     2521 ctcgtggggg ccggcaagga ggcggaggcc tgcggtctgc tcggggcgat cgaggagcgg
     2581 cacctggcct gtgcgcggtc gttccttacc ggcgcgaccc tgagccgctg tgaggcggat
     2641 ctgcgagcgg tctgcgggga gctgggcgcg ctgatcaagg cgctgtccct cctgaaggag
     2701 tggtccaagc gcagcaacga cgccattctt tccttcggag agcggctctc gacgatagtc
     2761 ctgctgcatc gcgcccggga gagagggatc gacgcggagc tgcacgactc ccgcgagctg
     2821 gtcaagacgg acgacagctt cacggccgcg gctcccctgg aggacctgac caaccgcctg
     2881 gtcggatcga aggtgcgcgc ccggcccggg cacctggcca tcctccaggg cttcatcgct
     2941 tccacggtta cgggcgccat caccacgctg ggccgcggcg ggtcggacta tacggcgacc
     3001 attgtcggag cggcgcttcg ggccgaagag gtgcaaatct ggaccgatgt gacgggcatc
     3061 atgacctcgg atccccggat cgttccgagc gcccggacga tcgaccagat cagctatcgc
     3121 gaagccgcgg agctcgctta cttcggcgcc aaggtgatcc atccgtcgac catccagccc
     3181 gcggtcaacc tggcgattcc ggtctgggtg aagaacacgt tccgccctca ggacgcgggc
     3241 acccgcatca gcgcctccat tccggggagc gggatgcggg ccatcgcctc caagaagggg
     3301 atcaccgtgg tgagcgtgag ctcctcccgc atgctcctgg catacggctt cctgcgcagg
     3361 atcttcgaga tcttcgagaa gcacgagacg ccggtcgatc tgattgccac ttcggaagtg
     3421 tccgtgtccg tcaccataga cacgccgacc gccgtagacg ccattgcacg ggacctggcc
     3481 gagatcggga cggtcggggt g
//
LOCUS       DASRZS010000667         4247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5383568, whole genome shotgun sequence.
ACCESSION   DASRZS010000667 DASRZS010000000
VERSION     DASRZS010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5383568"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFH83_16240"
     CDS             complement(<1..464)
                     /locus_tag="VFH83_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002679608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6487978.1"
                     /translation="MKERIASPGLNEGEDRDDQKLRPRLLTEFVGQEKLKSNLAVYIE
                     AAKQRGEHLDHVFLSGPPGLGKTTLAAIVANELGVQFRATSAPALERPGDLAAILTSI
                     GDRNIFFIDEIHRLKPVVEELLYSAMEDFEIDVVIGQGPGARTIKIPLPPFTL"
     gene            complement(461..1066)
                     /gene="ruvA"
                     /locus_tag="VFH83_16245"
     CDS             complement(461..1066)
                     /gene="ruvA"
                     /locus_tag="VFH83_16245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET6487979.1"
                     /translation="MFNSITGEISHKGEERLCIQTAGIEWDLAVSRKALERLPAVGEV
                     ARVFTYLVHREDVMRLYGFADAAERSLFLDLQKVEGVGPRGAMKMLSGVDREQFVSAL
                     DREDVEALSGIPGIGKKTAQKIILTLKGKLTTSVEPAGRSTEEDIATALVGMGFDRKT
                     ARAAVAAALRDVTDPGLSAEEREREVIRRAVAIAGGEGTAG"
     gene            complement(1044..1541)
                     /gene="ruvC"
                     /locus_tag="VFH83_16250"
     CDS             complement(1044..1541)
                     /gene="ruvC"
                     /locus_tag="VFH83_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET6487980.1"
                     /translation="MRTLIGLDPGLASTGWGVVRFDGSRFHHVAHGVVHTEPDTPLET
                     RLCSIHAALRKLLARYQPDEAGVEELYFAKNVSSAIHVAQARGVVLLALGQRGIPVSC
                     YTPQHVKQAVIGRGKAEKDQVQRLVSVLLGLTQIPSPDHAADALAIAICHANRSALDV
                     QQHHR"
     gene            complement(1538..2281)
                     /locus_tag="VFH83_16255"
     CDS             complement(1538..2281)
                     /locus_tag="VFH83_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6487981.1"
                     /translation="MSGHSKWHTIKHKKGVADAKRGQTFTKLIKEISISARLGGGDPE
                     ANPRLRTVILKAKAANMPKDNIDRAIKKGTGELEGVNYEEILYEAYGPGGVAILIDCL
                     TDNKNRAAAEIRNLLNKGGGNLGAAGSVAYLFKRKGLINYDPAKYKEDDVLAAALEAG
                     AEDVSNDGDSIEVTTSPEDFEQVVKALETAGFQHSMAELARVPDNTVTLDNDKTQKAL
                     RLVEHLEDHEDVQSVATNLEIPAGFVMSE"
     gene            complement(2379..3611)
                     /locus_tag="VFH83_16260"
     CDS             complement(2379..3611)
                     /locus_tag="VFH83_16260"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HET6487982.1"
                     /translation="MDSEPSDPATGLYVHVPLCTDKCLYCDFYSVPRSAVPLRDQEEL
                     VRQIGLQASTLLSQLEGSVRTIFVGGGTPSALPGRAVSTLLRSLPGAAAEEWTVEANP
                     ETIDAPFLEACRESGVTRLSVGVQSTDDRLLATLRRRARRADVERTLKLLDSSWGGEL
                     SFDLITGIPGQKPTSVVDDIGVLAGSGCGHFSLYQLTCEPDTPLARSVAEGTIRLNEP
                     EQDEELWFAGVAELRRRGFQHYEISNFCREGKECRHNLRYWRMEPYLGVGPAAVSTLP
                     AARVGPVLRRQSATGILRLTNPRSVTRYLQGAPALWGIEVEEIAPRDFLLETLMMGLR
                     LKDGLEARGFHHRFGADLDELAPGVWNRWVSRGLALRAADRLALTDSGRFVLDGLLIE
                     LANDLQSRELSGLSVSWP"
     gene            complement(3592..>4247)
                     /gene="lepB"
                     /locus_tag="VFH83_16265"
     CDS             complement(3592..>4247)
                     /gene="lepB"
                     /locus_tag="VFH83_16265"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET6487983.1"
                     /translation="LLFLVVTRFLVSTFRIESVSMEPTLVPADRVVVSLLAYGPRLPL
                     SPARLPGLGRPQRGDLVVVQPPFLDAFSLSSRLIEPLAGFFTLQKATLHRQLSGSRVN
                     AYMVKRVVGLPGDTVRLKDYVLSVKPRGSAYFIQETNLAPRPYRTLQTPPGGDWPASV
                     ALPGLGTDLTLGDGQYFVLGDDRPLSSDSRSWGPLDGSRIIGKVILRYWPPRGFGTF"
BASE COUNT          706 a         1411 c         1372 g          758 t
ORIGIN      
        1 agggtgaagg gaggaagcgg gatcttgatg gtgcgcgcgc ccggtccctg gccgatcacg
       61 acgtcgattt cgaagtcctc catggccgag tacaacagct cttcgaccac gggcttcagg
      121 cggtggatct cgtcgatgaa gaagatgttc cgatcgccga tgctggtcag gatggccgcc
      181 aggtcgcccg gccgttccag ggctggggca gacgtggcgc gaaactggac ccccagctcg
      241 ttcgctacga tggccgcgag cgtcgtcttg cccagccccg gcgggccgga caggaagacg
      301 tgatccaggt gctcgccacg ctgtttggcc gcctcgatgt ataccgcgag gttcgacttc
      361 agcttctcct gcccgacgaa ctccgtaagg agccggggac gaagcttctg gtcgtcgcgg
      421 tcctccccct cgttcaggcc aggactggcg atgcgctcct tcatccggcc gtcccctcac
      481 cgcccgcgat ggccacggcc cttcggatga cctcccgctc cctctcctcc gcgctgagcc
      541 cgggatccgt aacatcccga agcgccgcag ccacggccgc gcgggcagtc ttgcggtcga
      601 accccatccc caccagggcg gtggcgatgt cctcttcggt cgatcggccg gcgggttcca
      661 cgctcgtggt aagcttgcct ttgagcgtca ggatgatttt ctgggccgtc tttttcccta
      721 tcccgggaat ccccgagagc gcttcgacgt cctcgcggtc cagggcggag acgaactgct
      781 cgcggtccac cccggagagc atcttcatgg ccccgcgcgg tccgactccc tcgaccttct
      841 gcaggtcgag gaaaagcgag cgctccgcgg catccgcgaa gccgtacagt cgcatgacgt
      901 cctcgcggtg gaccaggtag gtgaacacgc gggcaacctc tccgaccgcg ggcaggcgct
      961 ccagcgcctt ccgcgacacc gccaggtccc actcgatgcc ggcggtctgg atgcagaggc
     1021 gctcctcgcc cttgtggctt atctcaccgg tgatgctgtt gaacatcgag ggccgaccgg
     1081 ttggcgtgac agatggcaat ggccagggcg tccgcggcgt ggtccggcga cgggatctgg
     1141 gtcaggccaa ggaggacgct caccagtcgc tggacctgat ccttctccgc cttcccgcga
     1201 ccgatgaccg cctgcttcac gtgttgcggc gtgtagcagc tcaccggaat cccgcgctgc
     1261 ccaagggcca gcaggacgac tccgcgcgcc tgggctacgt ggatcgcgct cgaaacgttc
     1321 ttggcgaagt agagctcttc cacacccgcc tcgtcgggct ggtaacgggc aagcagcttc
     1381 cgcagggccg catggatcga gcacagccga gtctcaagag gcgtatcggg ctcggtgtga
     1441 acgacaccgt gagcaacgtg atggaatcgg gaaccgtcga accgcacgac gccccagccc
     1501 gtggaagcca ggcccggatc cagcccgatc agcgtcctca ttcactcatg acaaatccgg
     1561 cgggaatctc cagattcgtc gcgacgctct gcacatcctc gtggtcttcc agatgctcga
     1621 cgagccggag cgctttttgg gtcttgtcgt tgtccagggt aaccgtgttg tccggcacgc
     1681 gggccagctc cgccatggag tgctggaacc ccgccgtctc cagggccttg accacctgct
     1741 cgaagtcttc cgggctcgtc gtgacttcga tgctgtcccc gtcgttgctc acgtcttccg
     1801 cgccagcctc cagggccgcg gccaggacat cgtcctcttt gtacttggcc gggtcataat
     1861 tgatcaggcc cttgcgtttg aacagatacg ctacgcttcc ggccgcgccc aggtttcccc
     1921 cgcccttgtt gaggaggttg cggatctccg ccgcggcgcg gttcttgttg tcggtgagac
     1981 agtcgatgag gatcgccaca ccgcccggac catacgcctc gtagaggatc tcctcgtagt
     2041 tcacgccctc cagctcgccg gtgcccttct tgatggcccg gtcgatgttg tccttgggca
     2101 tgttggcggc cttcgccttg aggatgaccg tccgcagcct gggattggcc tccggatcac
     2161 ccccaccgag acgcgcggag atactgatct ccttgatgag cttggtgaag gtctggccgc
     2221 gctttgcgtc cgcgaccccc ttcttgtgct tgatggtatg ccatttgcta tggccagaca
     2281 tgcccgtcct tctcctgcga aagtttacga aaccgtatca cgaacggaaa aggctcgtca
     2341 atacgagagg gtccggcagg agagggtccg cgggccgatc agggccacga cacggagaga
     2401 ccgctcagct cccggctctg caggtcgttg gcgagctcga tcagcagacc gtccaggacg
     2461 aagcggccgc tgtctgtcag cgccagccgg tcggcggcac ggagcgccag tcctcgggat
     2521 acccagcggt tccagacccc cggggcgagc tcgtccaggt cggcaccgaa gcgatggtga
     2581 aaacccctcg cttccagccc gtccttgagg cgaagcccca tcatgagggt ctccagcagg
     2641 aagtcgcgtg gcgcgatctc ttccacctcg atgccccaca gcgccggtgc tccttgaagg
     2701 taccgcgtga cactccgcgg gttggtcagt cgcaggatcc cggtcgcgct ctggcggcgc
     2761 agcacgggtc cgacgcgcgc tgctggcagc gtcgagaccg ctgccgggcc taccccgaga
     2821 tacggctcca ttcgccagta tctcagattg tgccggcact cctttccttc ccggcagaag
     2881 ttggagatct cgtagtgctg gaatccgcgc ctcctcagct ccgccacgcc ggcaaaccag
     2941 agctcctcgt cctgttccgg ttcgttcaac cggatggtgc cctccgccac cgatcgcgcc
     3001 agcggcgtgt cgggttcaca ggtgagctgg tagagcgaga agtgcccgca gccggagccg
     3061 gcgagcacgc cgatgtcatc gacgacggac gtgggcttct ggccggggat gccggtgatg
     3121 aggtcgaagc tcagctcgcc gccccagctc gagtccagga gcttgagcgt gcgctcgacg
     3181 tcggcgcgtc gtgcccgcct tctcagcgta gcaaggagtc gatcgtccgt gctctgcacg
     3241 ccgacgctca gccgggtaac cccggattcc ctgcatgctt cgagaaacgg cgcatcaatg
     3301 gtctcgggat tggcctcgac ggtccactct tctgccgctg ctccgggaag ggaccgaagc
     3361 agcgtcgaga ccgccctgcc gggcagggcg ctgggggtgc cgccgccgac gaagatcgtg
     3421 cggacggagc cttccaactg gctcagaagg gtcgaggcct ggaggccgat ctggcggacc
     3481 agctcctcct ggtcccgcaa gggcaccgcc gatcgtggaa cggagtagaa atcgcagtac
     3541 agacacttat cggtgcaaag cgggacgtgg acatagaggc ccgttgcggg atcagaaggt
     3601 tccgaatcca cggggcggcc aataccgaag gatcaccttg ccgatgatgc ggctgccgtc
     3661 gagcggaccc caggagcggc tgtcgctgga aaggggacgg tcgtcgccca gcacgaagta
     3721 ctgcccatcg cccagcgtga gatcggtacc caggccgggt agggccaccg aggccggcca
     3781 gtcaccgccc ggtggcgtct ggagcgttcg gtacggtcgc ggcgccaggt tcgtctcctg
     3841 gatgaaatag gcgctgcccc ttggcttcac ggaaaggacg tagtctttga gccggaccgt
     3901 gtcccccggc agcccgacga cacgcttgac catgtaggcg ttcaccctgg accccgagag
     3961 ctgtcggtgc agcgtggcct tttggagggt gaagaagccg gccaggggct cgatcaaccg
     4021 ggaagacagg ctgaatgcat ccaggaatgg aggctgcacc acaacgaggt cgccccgttg
     4081 aggccgtccc agcccgggga gacgggctgg gctcagggga agccggggac cgtacgccag
     4141 gaggctcacc accacgcgat ccgccggcac gagcgtcggc tccatggaca cggactcgat
     4201 acggaacgta gataccagga atcgggttac gaccaggaac aggagta
//
LOCUS       DASRZS010000668         4071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4596484, whole genome shotgun sequence.
ACCESSION   DASRZS010000668 DASRZS010000000
VERSION     DASRZS010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4596484"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFH83_16270"
     CDS             <1..325
                     /locus_tag="VFH83_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6487984.1"
                     /translation="ISKSKARYRGDPTKARAKLAFLDVGPSLQLELIQPDDEPSTWRE
                     DLNRKGEGVHHIAFKIKGMKDKTADLEKEGMTLLQTGEYTGGRYAYIDATRDLKVIVE
                     LLEND"
     gene            322..1290
                     /locus_tag="VFH83_16275"
     CDS             322..1290
                     /locus_tag="VFH83_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6487985.1"
                     /translation="MKRPDAGPVPAPFSVPQGLGLGCWAFGDMGTGIRNDDGSIALIR
                     EAIGLGVIHLDTAQSYGEGHSEEIVGRAVRDGVGGRRDAVFIATKAHATSATETEATV
                     VRSLARMGLDRLDLFYIHWPHKGLDSRPMVEALERLRARGSIRGIGVSNFSVEQMEQV
                     SQVGRIDAHELCYNLLWRYPERDVIPYCRQKGIALVTYSTIAQGLLSDTPRGPEAFAP
                     GDARARTLYYRSDVWPRLRPSVEAMQQAARRHGAPLSDAALQWVLAQAGIRASLVGAR
                     SRDQLASSTAAARAPLDPALAAELQGLSAEAMRVIPDEGNIFLYYP"
     gene            complement(1316..>4071)
                     /locus_tag="VFH83_16280"
     CDS             complement(1316..>4071)
                     /locus_tag="VFH83_16280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET6487986.1"
                     /translation="VSLVTAGLTVAIAAALAPRILRLRSERQRAQSREKESARILANL
                     IESTTDAFFVKDRDLRMVLCNSVQAAFVGKQPAELYGKSDLESGWDSDQVKGNPQRGI
                     RGWEQDDRAALAGRTVRSVEQVTGSGEVRYLDTLKVPLRDERGDVVGVIGLSRDITDR
                     KRMEEDLRESQERLRQIFEHAPVAIAVLDRDMRYLMVSRRWLTDFGLTQAEVVGRSHY
                     EMFPDIPERWKEIHRRCLAGATESADEDPFPRADGTVEWVKWEIRPWFTQTKEIGGIY
                     IFSENLTQRRQLQQILQKERSMLLTLVNGLPDRIFAKDRESRFILANQAAASSMGVGE
                     PSDLLGKTDHDFLDAAVADPFRAMEKRIVEEGRGLINFEESGRNASGTFWTLTTKLPF
                     RDESGRIAGLVGIGRDITEQKLASQKLAEQAALLDITADAVLVRDLDDRIVYWNRGAE
                     RMYGWPRQEALGKRVAELIGEDMPDLASAHRQVLERGSWEGEFRRKTREGRILRIEAR
                     WNLLRDEAGAPKGILSVNTDVTERRAIQAQFFRVQRLESLGTLAGGIAHDLNNVLAPI
                     LMGIEGLSLRHQDDDNRRITDIMRSAAQRGAGVIRQILSFARGASGERAEVQLRHVVS
                     EIEDMIRETFPRSIEIRSEVTKDLSPVLADATQMHQVLMNLCVNARDAMPGGGTLFLA
                     GRNVRLDEEFVRHHIEAQPIPYVMIEVRDTGTGMLPEVQEKIFEPFFTTKDPGKGTGL
                     GLSTTRTIVKSHGGFLSFYSEPGHGTSFRVYIPAANAADRSQDAGSTQALPEGRGQLI
                     LVVDDEPSIREMCLLILQDHGYEVMTATGGEQALAAFSANSSRVRLVITDMMMPGMDG
                     AEVIRGIRRMDPRVRIIASSGLAAWGESPETATLGVNGFLPKPYTAETLLGTLGAVLG
                     SDA"
BASE COUNT          645 a         1408 c         1312 g          706 t
ORIGIN      
        1 catctccaag tccaaggcgc gctatcgggg cgaccccacc aaggcgcggg ccaagctcgc
       61 gttcctcgat gtggggccgt ccctccagct cgagctgatc cagccggacg acgagccaag
      121 cacctggcgt gaggatctga accgcaaggg ggaaggggtc caccacatcg cgttcaagat
      181 caaagggatg aaggacaaga ccgccgacct cgagaaggaa ggcatgacgc tcctccagac
      241 cggcgagtac accggcggcc gctacgccta catcgacgca acccgggatc tcaaggtcat
      301 cgtcgagctc ctggagaacg attgaagcga ccggacgccg gacccgtgcc cgcccccttc
      361 tccgtacccc aaggcctggg tctggggtgc tgggccttcg gtgatatggg gaccggcatc
      421 cggaatgacg atggctcgat cgcgctgatc cgggaggcca tcgggcttgg cgtcatccac
      481 ctggacacgg cccagagcta cggcgagggg cactcggagg agatcgtcgg ccgcgcggtg
      541 cgggacgggg tgggaggccg gcgggacgcg gtcttcatcg ccaccaaggc gcacgcgacg
      601 agcgccacgg agacggaggc aaccgtcgtc cggtctctgg cccgcatggg gctggatcgc
      661 ctggacctgt tctacatcca ctggccccac aagggtctgg actcgaggcc gatggtggag
      721 gcgctggagc ggcttcgagc gcgcgggtcg atccgaggca tcggggtcag caacttcagc
      781 gtcgagcaga tggagcaggt atcccaggtg gggcgaatcg acgcccacga gctctgctac
      841 aacctcttgt ggcgctaccc ggagcgggac gtcatcccgt actgccgtca gaagggcata
      901 gccctggtga cctacagcac catcgcgcag ggattgctct ccgacacgcc tcgaggtccc
      961 gaggcgttcg ctcccggcga cgcgcgcgcg cggacgctgt actaccggtc cgacgtctgg
     1021 ccgcggcttc gtcccagcgt ggaggccatg cagcaggcgg ccagacgtca cggggcgccg
     1081 ctgtcggatg cggcgctgca gtgggtgctg gctcaggccg gcatccgcgc atccctggtc
     1141 ggcgcccgca gccgggacca actggcgagc agcacggcgg ccgcgcgcgc gccgctggat
     1201 ccggccctgg cggcggagct ccaggggctc tcagcggagg ccatgcgcgt catcccggac
     1261 gaggggaaca tcttcctcta ctatccgtaa tggttcccgt agcgagcccg tagtctcacg
     1321 cgtccgaccc caggaccgct cccagcgtcc ccaagagcgt ctccgccgtg tagggcttgg
     1381 ggaggaaccc gttcactccc agggtggcgg tctccggaga ctcgccccag gcggcaagac
     1441 cgctggaggc gatgatcctg acccgcggat ccatccgacg tatcccccgg atgacctcag
     1501 ccccgtccat ccccggcatc atcatgtccg tgatgaccag gcggaccctg ctgctgttgg
     1561 ctgaaaaggc cgcgagcgct tgctccccgc ccgtcgcggt catcacctcg tagccgtggt
     1621 cctggaggat caggagacac atctcgcgga tcgacggctc gtcgtccacc accaggataa
     1681 gctggcctct cccctcggga agcgcctggg tggagccggc gtcctgggag cgatcggccg
     1741 cgttcgcggc agggatgtag acgcggaacg acgttccatg ccccggctcg ctgtagaagc
     1801 tcaggaaccc gccgtggctc ttcacgatgg ttcgggtggt ggagaggccg aggcccgtcc
     1861 ccttccccgg atccttggtg gtaaagaacg gctcgaagat cttctcctgt acctcgggga
     1921 gcattccggt tccggtatcc cgtacctcga tcatcacgta cgggatcggc tgcgcctcga
     1981 tgtggtggcg aacgaactcc tcgtccaggc ggacgttcct ccctgccagg aacagcgttc
     2041 caccgcccgg catggcgtcg cgtgcgttca cgcagaggtt catcagcacc tggtgcatct
     2101 gcgtggcgtc cgcgagcacc ggtgagagat ccttcgtgac ctcggagcgg atctcgatgg
     2161 atctcggaaa ggtctcgcgg atcatgtcct cgatctcgga caccacgtga cgaagctgga
     2221 cctccgccct ctctccgctc gccccgcgcg cgaagctcag gatctggcgg atcactcccg
     2281 cccctcgctg cgccgcgctt cgcatgatgt cggtgatccg gcggttgtcg tcgtcctggt
     2341 ggcgaaggct gaggccctcg atccccatga ggatgggggc caggacgttg ttgagatcgt
     2401 gggcgattcc tcccgcgagc gttcccaggc tctccaggcg ttggacccgg aagaactgcg
     2461 cctggatggc gcgccgctcc gtgacgtccg tgttcacgga gaggatgccc ttgggcgcgc
     2521 cggcctcgtc ccgcaggagg ttccagcgag cctcgatgcg caggatgcgc ccctcacggg
     2581 tcttccggcg gaactctcct tcccaggatc cgcgttccag cacctgccgg tgagccgacg
     2641 cgagatccgg catgtcctcc ccgatgagct ccgccaccct cttgcccagc gcctcctggc
     2701 gcggccagcc gtacatccgc tcggcgcccc tgttccagta gacgatccgg tcgtccaggt
     2761 cccggaccag aacagcgtcc gccgtgatgt ccaggagggc cgcctgctcc gcgagcttct
     2821 gggaggcgag cttctgctcc gtgatgtcgc ggccaatgcc aaccaggccg gcaatccgac
     2881 cgctctcgtc gcggaacggc agcttggtgg tgagagtcca gaacgttccg gacgcgttgc
     2941 gtcctgactc ctcgaaattg atcaggcccc ggccctcctc gacgatccgc ttctccatcg
     3001 cgcgaaacgg atcggcgacg gcggcgtcca ggaagtcgtg atccgtcttt cccaggaggt
     3061 cggagggctc tcctaccccc atcgagcttg cggccgcctg attggccagg atgaaccggc
     3121 tctcgcggtc cttggcaaag atccggtccg ggagcccgtt gaccagcgtg aggagcatgg
     3181 atcgctcctt ctgcaggatc tgctggagct gccggcgctg cgtcaggttc tcactgaaga
     3241 tgtagatccc gccgatctcc ttggtctggg tgaaccacgg gcggatctcc catttcaccc
     3301 actccaccgt gccatccgcg cgggggaacg ggtcctcatc cgcgctttcc gtcgcaccgg
     3361 cgaggcacct gcggtgaatc tccttccatc gctcagggat gtccgggaac atctcgtaat
     3421 ggcttctgcc caccacctcg gcctgggtga ggccgaagtc ggtcagccat cggcggctca
     3481 ccatcaggta tcgcatgtcg cggtccagga ccgcgatggc cacgggagcg tgctcgaaga
     3541 tctgacggag cctctcctgg ctttcccgga ggtcctcctc catcctcttt cgatccgtga
     3601 tgtcccggct cagcccgatc acccccacca cgtcaccccg ctcatcccgc aaaggcacct
     3661 tgagcgtgtc caggtaacgc acctccccgg agcccgtgac ttgctccacg ctgcgcacgg
     3721 ttcgtccggc cagcgccgca cgatcgtcct gctcccaacc ccggatcccg cgctggggat
     3781 tgcctttcac ctggtccgaa tcccagccgc tctccaggtc cgacttcccg tagagctccg
     3841 cgggctgttt cccgacaaag gcggcctgga ccgagttgca gagcaccatg cgaaggtcgc
     3901 ggtccttgac gaagaacgca tccgtggtgg actcgatcag gttggcgagg atccgggcgc
     3961 tttccttctc tctggattgg gctcgctgcc gttcggaacg caacctgagg atccggggcg
     4021 ctaacgcggc ggcaatggcc acggtcagcc ccgcggtgac cagggatacc g
//
LOCUS       DASRZS010000669         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3440433, whole genome shotgun sequence.
ACCESSION   DASRZS010000669 DASRZS010000000
VERSION     DASRZS010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3440433"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VFH83_16285"
     CDS             complement(<1..687)
                     /locus_tag="VFH83_16285"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET6487987.1"
                     /translation="MKKQNIVAQVYTVRDACNDAHGLADALARVRAIGYQAVEMSSVG
                     PLPPKELARVLEGEGLTCAGTHEDSEELLADPSSVAERLAILGCTATAYPYPKGQDFS
                     SLEAVKRLCKALERTGKTLKKAGISFSYHNHNLEFFRLGKSKVIDILLSETDDEAVKL
                     ELDTYWVQAGGGNPAEWIGKLKGRCSHLHIKDYGVDPKGRAMFEEIGKGNLDWDGIFA
                     AAKKAGVKWYV"
     gene            862..2553
                     /locus_tag="VFH83_16290"
     CDS             862..2553
                     /locus_tag="VFH83_16290"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET6487988.1"
                     /translation="MEFLEQVPREILSWQLKGSGLENLGSKGRPETVPFPRYTEDDLI
                     ARIDAVGLCFSDIKLISAGNTHPRIEGRDLAKNPTVPGHEISMTIVGIGDNRKGQFAL
                     GQRCIIQADVYYKGKGLAFGYAIPGGLSQYVVIGKEILDGDEGCYLLPVKTSTGFAEA
                     ALVEPWTCVTAAYQIRARTQPRTGGHLTFVGFAKGQIPLDLGGLEAARPAVVSHSGLS
                     DANLKAVEELAGKSGARLVARGSDSGIAAPADIVCAGTPDRESFAALVNEIDADGVLG
                     VHTSAPEASLPVDVGKVHYRKLELVGSTDGGVARSYRANNRENLLPGARAWFVGGAGP
                     MGQMHVIKAIMDEKGPGHALVSDLSDDRLASLKHLVSLLTSKRQRQVTLEFENPKDLP
                     EDELEEFLRKRFPGGFEDVVVLVPVAAIISQAAHFVAPGGVLNIFAGVKVGTIADLPL
                     AAIVRDHVRVIGSSGSPLSAMRDTLALTEGGQLSTSYSLAAVGDMGSAGRGLKALMDN
                     TYTGKVVIFPFAHGMGLRSLKELARELPELSGRLLDGQYWTREAEDVFFKSKFFS"
     gene            2567..>2830
                     /gene="fucI"
                     /locus_tag="VFH83_16295"
     CDS             2567..>2830
                     /gene="fucI"
                     /locus_tag="VFH83_16295"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635899.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HET6487989.1"
                     /translation="MANVAGMKVTQPVNRLPGGLPRIGIRPTIDGRRKGVRESLEAQT
                     MAMARSVAKLLTESLRHPCGLPVECVIADTCIGGVAESARAAEK"
BASE COUNT          464 a          923 c          951 g          492 t
ORIGIN      
        1 cacgtaccat ttgacccccg ccttcttggc ggcggcaaag ataccgtccc agtcgaggtt
       61 gcccttcccg atctcttcga acatcgcccg gcccttggga tccacgccgt agtccttgat
      121 gtgcagatgc gagcatcgtc ccttgagctt gccgatccac tcggccgggt tcccgccgcc
      181 ggcctggacc cagtaggtgt ccagctcaag cttcacggct tcgtcgtccg tctcagaaag
      241 cagaatgtcg atgactttgc tcttgccgag gcggaagaac tccaggttgt ggttatggta
      301 ggagaacgag atccccgcct tcttgagcgt cttcccggtg cgctccagcg ccttgcagag
      361 cctcttcacg gcctccaggg aggagaagtc ctggcccttg gggtaggggt aggcggtcgc
      421 agtgcagccc aggatggcca accgctccgc cacggaggaa gggtcggcca gcagctcctc
      481 cgagtcctcg tgcgttccgg cgcacgtgag gccctctcct tccagcaccc tggccagctc
      541 cttgggcggg agcggaccga cgctgctcat ctccaccgcc tggtagccga tggcgcgcac
      601 ccgcgccagg gcgtccgcca ggccatgggc gtcgttgcac gcgtctcgaa cggtatacac
      661 ctgtgcgacg atgttctgct ttttcatctc cgatagccta ccacgagtga ggcggccgct
      721 gcaagcgaac ggagatccgg gcgcggttgc ctgccgcggg ccgccatgct acacttgaac
      781 gtgcaggctg gccgtgcggg gcccagattc gaccgtcgga cccgcgcaag gcgggggacc
      841 tacgcctgca aggagcacag tatggagttc ctcgagcaag taccccggga gatcctctcc
      901 tggcagttga agggctcggg gcttgagaac cttggaagca agggccggcc cgagactgtt
      961 ccgtttccgc gctacacgga agacgacctg attgcccgca tcgacgccgt ggggctgtgc
     1021 ttctccgaca tcaagctcat ctccgcgggc aacacccatc cccgcatcga ggggcgggat
     1081 ctggccaaga atcccacggt cccgggacac gagatctcca tgaccatcgt cgggatcggt
     1141 gacaaccgca aagggcagtt tgccctgggc cagcgctgca tcatccaggc ggacgtgtac
     1201 tacaagggaa agggtttggc gttcgggtat gccatccccg gtggcctctc ccagtacgtg
     1261 gtcatcggca aggagatcct ggacggtgat gaaggctgct acctccttcc cgtgaagacc
     1321 tccacgggat tcgccgaggc ggccctggtg gagccgtgga cgtgcgtgac ggcggcatac
     1381 cagatccggg cccggaccca gccgcgcacg ggtggtcacc tgaccttcgt ggggttcgcc
     1441 aagggccaga tccccctgga tctgggtggt ctggaggcgg cccggccggc tgttgtttcc
     1501 cactccggcc tctcggatgc caacctcaag gccgtggagg agctcgccgg caagtctggc
     1561 gctcggctgg tggcgcgagg ctccgacagc ggcattgcgg cccctgcgga catcgtctgc
     1621 gccggcacgc cggaccgtga gagcttcgcc gcgctggtga acgagatcga tgcggacggg
     1681 gtgctcggcg tgcacacttc ggcgcccgag gcgagcctgc ccgtggacgt gggcaaggtg
     1741 cactaccgca agctcgagct cgtcggctcc acggacggcg gcgtggccag gagctaccgc
     1801 gccaacaatc gggagaatct gctgcccgga gcccgcgcat ggtttgtggg cggagcgggc
     1861 cccatgggcc agatgcacgt gatcaaggcc atcatggatg agaagggccc cggccacgcc
     1921 ctggtcagcg acctctcgga cgatcggctg gcgtcgctga agcacctggt gtccctgctc
     1981 accagcaagc gacagagaca ggtgaccctg gagttcgaaa accccaagga cctgccggag
     2041 gacgagctgg aggagttcct ccgcaagcgc ttcccgggcg gcttcgagga cgtggtggtg
     2101 ctggtgcccg tggccgccat catcagccag gcggcgcact tcgtggcgcc gggaggcgtg
     2161 ctcaacattt tcgccggcgt gaaggtcggc accatcgcgg atcttccgct ggccgccatc
     2221 gtccgggacc acgtgcgcgt catcggctcc tccggctcgc ccctctccgc catgcgggat
     2281 acgctggcgc tcaccgaagg cggccaactc tccacgtcgt actctttggc cgcggtcggc
     2341 gacatgggct cggcgggccg cgggttgaag gcgctcatgg acaacaccta tactgggaag
     2401 gtcgtgatct tccccttcgc ccatggcatg ggcttgaggt ccctgaagga gctcgccagg
     2461 gagctgcccg agctttccgg gcgtctcctg gacggtcaat actggactcg ggaagcggag
     2521 gacgttttct tcaagagcaa gttcttctca tgagaaggag caggcgatgg cgaacgtagc
     2581 ggggatgaag gtgactcagc cggtgaaccg gcttccgggc gggctgccca ggatcggcat
     2641 ccggccgacg atcgacggcc ggcgcaaggg cgtgcgggag tccctggaag cgcagaccat
     2701 ggccatggcc cgatccgtgg ccaagcttct caccgagagc ctgcgccatc cctgcggcct
     2761 tcccgtggaa tgcgtcatcg cggatacgtg catcggcgga gtagccgagt cggcgcgcgc
     2821 ggcggagaag
//
LOCUS       DASRZS010000670         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5757205, whole genome shotgun sequence.
ACCESSION   DASRZS010000670 DASRZS010000000
VERSION     DASRZS010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5757205"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..948
                     /locus_tag="VFH83_16300"
     CDS             34..948
                     /locus_tag="VFH83_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6487990.1"
                     /translation="MRRADIRKGLWVALFVGPGLLVVGVFILLPLFVSLFNSLFSWQQ
                     LIRGPFTGLENFRRILVAYPYRERFYSALGNNLKWFLVTMLVQNTTGLLFGYLLSRRI
                     AGSAFYKRVFFIPVLFSIVAVGFLWGLYLKPNGLVNSFLTLVGRGRWTHAWLGEEAIA
                     TFTVILVNIWRWVGFPSLVFLAAIDSLPGECFEAAYLDGAREWTTFRRIVVPLIVPAI
                     TVITVLTIIGSLNVFEQVYVMTGLDGAPNYSTDTIGTLFYRTAFGSVDSGNPQIGIGS
                     AIGVIIYVLTFVASIGSITATRSREVQL"
     gene            945..1799
                     /locus_tag="VFH83_16305"
     CDS             945..1799
                     /locus_tag="VFH83_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6487991.1"
                     /translation="MTTDLRLRRAGPAQVVVLGVAQLLMGLYAVLIFYPLFTMIVSSF
                     KTTREIFQHPFSLPTHFLLANYKTVWVDRGFGRYMANSLIVTLIAMAFVLLLGSMAAY
                     GLSRYQYRLRTILYMLFLSGIMLPLKAAIIPLFMLIKGMGLMNTRASIILIFLAMGLP
                     STVFILSGFMRSIPLELEHAARIDGCNDWSIFQRVVLPVTTPAIALVTIYNAVPLWND
                     FFFPLVFLQSDRLKTLPVGLTTFFGQYSTNWSLLFTGLSIAIIPMLLLYVFMSGYFIR
                     GLTAGAIK"
     gene            1814..>2109
                     /locus_tag="VFH83_16310"
     CDS             1814..>2109
                     /locus_tag="VFH83_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HET6487992.1"
                     /translation="MQGSRDSHPAAAEVAGLKERYRRNLEESVLPFWLRHSVDREYGG
                     YFCCLNRDGSVFDTDKFLWLQGRGVWMFSRLYTSYREDPAWLDYARGGAAFLKA"
BASE COUNT          327 a          708 c          614 g          460 t
ORIGIN      
        1 gcccctctcg gaccccgacg gagaggtgaa ccggtgagaa gggccgacat ccgcaaggga
       61 ctgtgggtag cgcttttcgt cggtcccggc ctcctggtcg tcggggtgtt catcctcctg
      121 cctctcttcg tgtctctctt caacagcctc ttcagttggc agcagctcat ccggggtcca
      181 ttcaccggcc tggagaactt ccgaaggatc ctcgttgcct atccgtatcg ggagcggttc
      241 tacagcgccc tgggcaacaa cctgaaatgg ttcctcgtga cgatgctcgt ccagaacacg
      301 acgggcctcc tgttcggcta tctgctgagc cggaggatcg ccggcagcgc gttctacaag
      361 cgcgtgttct tcatccccgt cctcttctcg atcgtcgcgg tcgggttcct gtggggcctg
      421 tacctgaaac cgaacggact ggtgaactct ttcctgacgc tcgtgggtcg cggccgctgg
      481 acgcacgcct ggttgggaga ggaagccatc gccacgttca cggtcatcct ggtcaacatc
      541 tggcgatggg tcggtttccc gtctctggtc ttccttgcgg caatcgacag cctcccgggc
      601 gagtgcttcg aggcagcgta cctggacggt gcgcgggaat ggacgacctt ccggcggatc
      661 gtcgttcccc tcatcgttcc cgccatcacc gtgatcacgg tcctgaccat catcgggagc
      721 ctcaacgtct tcgagcaggt gtatgtgatg accgggctcg acggcgcccc caactactcg
      781 accgacacga tcgggacgct cttctaccgc accgcgttcg gctccgtgga ttccggcaat
      841 ccccagatcg ggatcgggag cgccatcggc gtgatcatct atgtcctcac gttcgtggcg
      901 tccatcggat ccatcacggc aacccgatcg agagaggtgc agctatgacc acggacctgc
      961 gtctgcggcg cgccggtccc gcgcaggtgg tggtcctggg agtggcccag ctactcatgg
     1021 ggctctacgc cgtcctgatc ttctacccgc tcttcaccat gatcgtgtcc tcgttcaaga
     1081 cgacgcggga gatcttccag caccccttct ccttgcccac gcacttcctc ctcgccaact
     1141 acaagacggt ctgggtggac cggggattcg gccgctacat ggccaacagc ctgatcgtca
     1201 cgctcatcgc catggcgttc gtgctgctgc tgggatccat ggcggcgtac gggctctcgc
     1261 gctaccagta ccggctgcga accatcctct acatgctgtt cctgagcggg atcatgctgc
     1321 cgctcaaggc ggccatcatt ccgcttttca tgctcatcaa gggcatgggg ctgatgaaca
     1381 cccgggcatc gatcatcctg atcttcctgg ccatggggct gccgtccacg gtcttcatcc
     1441 tctccggttt catgcggtcc atcccgctgg agctggagca tgccgcgcgc atcgatgggt
     1501 gtaacgactg gtccatcttc cagcgcgtcg tcctgccggt caccacgccg gccatcgcgc
     1561 ttgtcacgat ctacaacgcg gtgcccctct ggaacgactt cttcttcccg ctggtgttcc
     1621 tgcagagcga ccggctgaag acgctccccg ttggcctcac cacgttcttc gggcagtaca
     1681 gcaccaactg gagcctgctc tttaccggtc tttcgatcgc gatcatccct atgctgctcc
     1741 tgtacgtctt catgtccggc tacttcatcc gcggcctgac cgccggcgcg atcaagtagc
     1801 ggaaaggaca cctatgcaag gatcccggga ctctcacccg gccgcggcgg aagtggcggg
     1861 tctgaaagag cgataccgcc gcaacctgga ggagagcgtc ctgcctttct ggctgcgtca
     1921 ctccgtggac cgggaatacg gcgggtactt ctgctgtctc aatcgtgacg gctcggtctt
     1981 cgacaccgac aagttcctgt ggctccaggg gcgcggggtc tggatgttct cccgtctcta
     2041 cacgtcctat cgcgaagacc cggcctggct ggactacgcc cgcggggggg cggccttcct
     2101 gaaggccca
//
LOCUS       DASRZS010000671         9312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_375544, whole genome shotgun sequence.
ACCESSION   DASRZS010000671 DASRZS010000000
VERSION     DASRZS010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375544"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFH83_16315"
     CDS             <1..585
                     /locus_tag="VFH83_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487993.1"
                     /translation="GRAALTLVEKGWDTTLRGGLAVDEGRRPFSPGAVIPQSLQADLD
                     MDRLLIRWTRGSLHQTLTAEKRVDWSPTGQRSETAKCSLAAKCRWAGLTAMAAAAVEV
                     PEALGLSGSASFELDRSGDGVGLEVGTVVRPSRSVLDATVSIRRRLQDSQVQAQCGVA
                     NLPMVPPPRGLKDCLVLKLGWRTSLRKVAEPDGD"
     gene            complement(637..1548)
                     /gene="murB"
                     /locus_tag="VFH83_16320"
     CDS             complement(637..1548)
                     /gene="murB"
                     /locus_tag="VFH83_16320"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET6487994.1"
                     /translation="MDTVRELAGKIKLGAPPRFEEPMAKHTSFRIGGPADLYLVPEDA
                     EELSNALSICRRDGVPCFLLGGGTNILVADRGVRGVVIDLSRVSRIEPEGQCISAECG
                     AEISRVSETALRMGLAGLEFAYALPGTVGGAVWMNARCYDREMSDVLRWVDWIDTDGS
                     RRRLAIDPKEWSYKRSPFQGNDWLILKAGLELTPGDPVEIQASMSDHRQDRERKGHFR
                     YPCAGSVFKNNRAFGAPTGKLVDSLGLNGRRIGGAQIAPYHGNIIINTDRAAAADVRE
                     LIELVEREVRVQLGFQLEREVLLVGDW"
     gene            1601..3052
                     /gene="cysS"
                     /locus_tag="VFH83_16325"
     CDS             1601..3052
                     /gene="cysS"
                     /locus_tag="VFH83_16325"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET6487995.1"
                     /translation="MELRLFNTLGRQIQPFVPIQPGKVGMYACGLTVYGYAHVGNLRT
                     YITEDVLRKTLESQGFDVRHVMNVTDVGHLTDDADDGEDKMVKGARERGKTVWEIADF
                     FTKAFFSDFEAVRCRMPTVVCKATDHIPDMIALIQRLEQRGYTYLAGGNVFFDISKFP
                     TYGQLALLDQQDLRAGARIEVDQGKRNPADFALWFTRGKFEHQSMLWDSPWGKGYPGW
                     HIECSAMSMKYLGEQFDIHCGGIDHVPVHHTNEIAQSEAATGKKWVNYWVHGEWLLMD
                     RAKMARSSGHSITLTTVREKGFDPLDLRYLCLNAHYRTQLAFTWEAMEGARAARQGLL
                     EKVAQLRAEAGETAVKPQGATVEYLDQFQSHILDDLNTPRCLADLWTLVRDTSVPAPQ
                     KLAAAIRMDGILELGLGAARAREVELDSESQLLLQEREKARKSRDFKRADEIRAQLLA
                     RGIEIQDSPSGPKLRTASGRKVEKTVTSDDSDC"
     gene            3055..3585
                     /locus_tag="VFH83_16330"
     CDS             3055..3585
                     /locus_tag="VFH83_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6487996.1"
                     /translation="MSNTEYARPPSFDEAYETLFPAIYRVSLRVTGSREIAEELCHEA
                     FIKLMDRKDLQADLDQAKFWLIRVVKNLSLNYEKRRGRERAAYSRLTHLAPSVAESSE
                     KQVMREETRTLVQHALDKLPYNLRAPLVFREYGGLSYREIGSILHISESNVKVRIFRA
                     RERLEKALSEGEEYVS"
     gene            3683..4132
                     /locus_tag="VFH83_16335"
     CDS             3683..4132
                     /locus_tag="VFH83_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487997.1"
                     /translation="MTERLEHTRRTLRADDPFDWQGARERVRLRIATRVAAPIPVFVR
                     PTIWRRQVSIPLPVAVLAAGLVIALGITLATSLTRSNFGMVRITKAPAGTEIQIAAPI
                     SDLESLLAKMGGDSSQESVLTLPKNVRLVPVGEPRMGLEGDFSGKKP"
     gene            4129..4632
                     /locus_tag="VFH83_16340"
     CDS             4129..4632
                     /locus_tag="VFH83_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6487998.1"
                     /translation="MIRPKAAVLPLLLALGAMQASAQDADIDPKEALRQLLSNALKVT
                     ISAEILPTTDKPVWNAESTNLTLPGRSIKVRLEGDNVRIYLICTPYVQDDGVVLLLAQ
                     GQVWLSDPPDKESKYYSTFYSIPVTFGKKVLFFPLGVSDSDTAKDNKDFFNIELQIKI
                     EPAAQKQ"
     gene            4643..6400
                     /locus_tag="VFH83_16345"
     CDS             4643..6400
                     /locus_tag="VFH83_16345"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HET6487999.1"
                     /translation="MTPENDAPGSEDRSSGDYPGPDAGPEGAAEQQQKPYQRILRSKG
                     FVPVTVLVALIAFVLLSSRLAGRPPQIDTITPSRGKPGTVLLISGRYFEAERGSSEVR
                     ISGIAPTSSDYLDWTDRQVSVRIPDGATSGIVYVITRSGRSGGLLWVNQDQIPVPASG
                     PSRPGEPYIKEIKPSAAQVGDTITITGMNFGLEKDNSEVYFTPAAGGDSVASAGFDSV
                     SMLPANQGDFDYLSWSDREITLRVPDGAASGNVVVKSDKAPSNPVYFELLGGVGTKLY
                     GSPRKYSVQYAMDAEVSAASGENTLYLWMPSIIQTPEQRRSHLVSEEPQPLAVRDGPR
                     LYSLGNVQKGSQARASLSWIFDRYTVETQVSLAKPIPAYDDSTDLYHTFTQPDALVPS
                     NNPDLIKAAAAATGGEKSPYLKARRVYDWLLGQVTYSETASNPLQVLRQKRADAFAFS
                     SVLCAMLRASGIPARMVSGYLVGETGQPTRRHFWDEFYLPTFGWVPVDPLLGDDPSLY
                     PMTVAADFDAHAYYFGNLDNQHVTFSKGLTSVNQMSPMGKTRQFSEFPYLVSIDAETV
                     GGVTLGGVTFEDLVVSGTY"
     gene            complement(6494..7726)
                     /locus_tag="VFH83_16350"
     CDS             complement(6494..7726)
                     /locus_tag="VFH83_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET6488000.1"
                     /translation="MASPLQLSIVNFKKNSYIIVEGKQNADCFYILRSGKVRVSKETE
                     VVEEEGGNILAPGDFFGVISTMSAHSHIETALALSDVSLISVARDQYGLLIERNAPVA
                     MKIIQSFSRKMRYLDEALTRLTFKNTASGDPSHLFKVAEYYARQSQYNQAYYAYYRFL
                     QNCPTDPNAEKAKERLAKIHPYAKAVYLEKASDDFTRTYPKNTMIFSENEPGSELYII
                     QKGVVKITKIVEDNEVMLALLKAGDIFGEMALLENKPRSASALANEDCSLMAVNKGNF
                     ERMVQTQAQLITRLTTLLAERLWSLYRQLANTLIQDPLGRLYDALLLHLERNRVPIVK
                     GGKYTFQLTPKELLNFVGLSNEKGAPFVRKLFDNKKMVLSDNKIATVDVEEIHKQSEY
                     YKHMERIERARRKAAAGV"
     gene            7859..8425
                     /locus_tag="VFH83_16355"
     CDS             7859..8425
                     /locus_tag="VFH83_16355"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6488001.1"
                     /translation="MRPADEHLRWTEHARTRLASCAFFQFYSSERRSNSGKSASFCLL
                     DAPDWVNIVPVLRDPSGEERFLMVRQYRHGADLITTEFPAGLLEPGEDPVAAAHRELR
                     EETGYRAGRMTLLGRVSPNPAFMNNWCSTYLAEDLVPAGEPNLDELEELSTVTLSARA
                     LEEGIGRGELVNSMVLVALLWYRLARSR"
     gene            8422..>9312
                     /locus_tag="VFH83_16360"
     CDS             8422..>9312
                     /locus_tag="VFH83_16360"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HET6488002.1"
                     /translation="MRLRISKPVKIAIEVVVIGLLVLLFFLVVDPARLRVYLALITLR
                     SIGGLLAIQLTLHLVGMVQWLLILRQAGVTSSAWEVFWTRLSGCAITSLTPSMYFGGE
                     PVRASMLKNPTVSYQTLYATIAVDKYVELFTKLPAAAIGFAMLIQLAQPGPALLILSA
                     AFILVFCAFFLYLMVKLFQGSRFVLRAAKWVLVPMARVRPRLAVKIQMALKEFTKDVS
                     RILKKKLSFALAMFFGLLLSAVEVLQIYYVLAVLGVYDLRRAFAIFFATVFQGLLSFI
                     PGNIGTMEGLNLFMFSILKLG"
BASE COUNT         1627 a         2973 c         2986 g         1726 t
ORIGIN      
        1 gggcgggcgg cgctgactct cgtggagaag ggctgggaca ccactctgcg cggcggcctg
       61 gcagtcgatg aaggaagacg gccgttctcg ccgggggcgg tgatcccaca atcgctccag
      121 gctgacctgg acatggaccg acttctgatc cggtggaccc gaggctctct gcaccagact
      181 ctcaccgcgg agaaacgggt tgactggagc ccgaccgggc agcggtcgga gaccgcgaag
      241 tgctccctgg cggcaaagtg ccggtgggcc ggtctcaccg ctatggctgc agcagccgtg
      301 gaggtacctg aggcgctcgg cctgtccggc tcggcgagct tcgaactgga tcgcagcggt
      361 gacggggtgg gccttgaggt ggggacggtg gtgcggcctt cccggtcggt cctcgatgcc
      421 acggtctcca tccgcagacg cctccaggac agccaggtcc aggcccagtg cggcgttgcg
      481 aatcttccga tggttccccc gccccgcggg ctgaaggatt gcctggttct caagctgggg
      541 tggcgcacca gcctgcgtaa agtggccgag ccagacgggg actgagctgg ggcggccagg
      601 gcggctcggg ctacgcttcg cccctccggc ggcggtctac cagtctccca ccaggagcac
      661 ctcgcgctcg agctggaagc cgagctgtac gcgaacctcg cgttccacca gctcgatcag
      721 ctcgcggaca tccgcggccg ctgctcgatc ggtgttgatg atgatgtttc catgataggg
      781 cgcgatctgg gcgcccccaa tcctgcgccc gttcagcccc agggagtcca cgagcttgcc
      841 cgtaggcgcc ccaaaggccc ggttgttctt gaagacactc ccggcgcagg gatatcggaa
      901 gtgccccttg cgctcgcggt cctggcgatg gtcgctcatg gatgcctgga tctccaccgg
      961 atccccgggg gtcagctcca gcccggcttt caggatcagc cagtcgtttc cctggaaggg
     1021 agatcgcttg tacgaccact ccttcgggtc gatcgccagc cgccgacgag agccgtcggt
     1081 gtcgatccag tccacccagc gcagcacatc gctcatctct cgatcgtagc agcgggcgtt
     1141 catccacacg gcgcccccaa cggtccccgg gagagcgtac gcgaactcca ggccggccag
     1201 acccattcgg agcgcggtct ccgaaacgcg cgagatctcg gcgccgcact cggcagagat
     1261 gcactggcct tccggttcga tacgggatac ccgcgagagg tcgatcacca cgcctcgaac
     1321 gccgcggtcc gccaccagga tgttggttcc cccgcccagc aggaagcacg gaacgccgtc
     1381 ccgccgacag atggacagtg cgttcgacag ctcttccgcg tcttccggca ccaggtacag
     1441 gtccgcgggg ccgccaatgc ggaagctggt gtgctttgcc atcggttctt cgaagcgcgg
     1501 aggagcgcca agcttgattt tccccgccag ttcccgtact gtatccacag cgaaattgta
     1561 gcttcggagc cctcgaaagg tctacacgca ggagaaatcg gtggaattgc ggcttttcaa
     1621 caccctgggc aggcagatac agccgtttgt tccgatccag ccagggaaag tcggcatgta
     1681 cgcatgcggt ctcacggtct acggatacgc gcacgtcggc aacctgcgca cctacatcac
     1741 cgaggacgtg ctgcgcaaga cgctggagtc ccagggcttc gacgtgcgcc acgtcatgaa
     1801 cgtcaccgac gtcggccacc tcacggatga cgcagatgac ggcgaagaca agatggtgaa
     1861 gggcgcgaga gagcgaggaa agaccgtctg ggagatcgcg gacttcttta ccaaggcctt
     1921 cttcagcgat ttcgaggcgg tgcgctgccg catgcctacc gtggtctgca aggccaccga
     1981 ccacatcccc gacatgatcg cgctgatcca gaggctggag cagcgcggct acacgtatct
     2041 cgccggcggc aacgtgtttt tcgatatctc gaagttcccc acctacggac agctcgcctt
     2101 gctggaccag caggatctgc gcgctggcgc ccgcatcgag gtggaccagg gcaagcggaa
     2161 ccccgcggac ttcgccctct ggttcactcg aggcaagttc gagcatcagt ccatgctctg
     2221 ggattccccc tggggcaagg ggtaccccgg ctggcacatc gagtgcagcg cgatgtccat
     2281 gaagtacctg ggggagcagt tcgacatcca ctgcggcggc atcgaccacg tgcccgtcca
     2341 tcataccaac gagatagccc agtcggaggc cgccaccggc aagaagtggg tgaactactg
     2401 ggtgcacggg gaatggcttc tcatggaccg cgccaagatg gctcgctcct cggggcactc
     2461 catcacgttg accaccgtcc gggaaaaggg gttcgatccg ctcgaccttc ggtacctctg
     2521 cctcaacgct cactaccgga cccagctcgc cttcacgtgg gaggccatgg agggcgcccg
     2581 cgcggccaga caaggcctgc tggagaaggt ggcgcagctt cgcgcggagg cgggagagac
     2641 ggccgtgaag ccgcagggag cgaccgtgga gtatctcgac cagttccaga gccacatcct
     2701 cgatgacctg aacacaccgc ggtgcctggc ggacctgtgg actctggtga gagacacgag
     2761 tgtgccggca ccacagaagc tcgcggccgc tatccggatg gacggcatcc ttgagctggg
     2821 gcttggtgcg gcccgcgccc gggaggtcga gctggactcc gagagccagc ttctgctcca
     2881 ggaacgggag aaggcgcgaa agtcacgtga cttcaagcgg gcggacgaga tacgcgccca
     2941 actgctggct cggggcatcg agattcagga cagcccgtcc gggccgaagc tacgtactgc
     3001 ctccggccga aaggtggaaa agactgtaac atcagacgat agtgattgtt aataatgagt
     3061 aacacagagt atgcgcgccc gccctctttt gacgaggcct atgagacact cttccctgcc
     3121 atctaccggg tttctttacg ggtgaccgga agccgggaga tagcggagga gctgtgtcat
     3181 gaggcgttca tcaagctcat ggatcgtaaa gacctccaag ccgacctcga tcaggccaaa
     3241 ttctggctca tccgcgtggt aaagaacctg tccctgaact atgagaagcg ccgcggccgc
     3301 gagcgcgcgg cctacagccg cctgactcac ctggcgccgt ccgtagcgga gtccagtgag
     3361 aagcaggtga tgcgcgagga gacgcggacg ctcgtccaac atgccctgga caagcttccg
     3421 tacaacctga gggcgcctct ggtctttcgc gagtatggtg gcttgagcta tcgtgagatt
     3481 gggtccattc tccatatcag tgaaagcaac gtgaaagtac ggatcttccg cgcgcgtgag
     3541 cgcctcgaga aggcgctgtc agagggtgaa gagtatgtgt cctgagcgcg atattctgtc
     3601 cgcttacgtc gacggcgagg tcccctctcc gtgggatcgg gccatagcgg atcacgtggc
     3661 cgggtgtgag tcctgcagaa gcgtgaccga acgactggag cacacccgac ggaccttgcg
     3721 agcggacgac ccctttgatt ggcagggagc ccgagagcgc gttcggctgc ggattgccac
     3781 tcgggtggcg gccccgatcc cggtgttcgt ccgacccacg atctggcgca ggcaggtgag
     3841 catccccctt cccgttgccg tcctggccgc gggtctcgtg atagcgctgg gaatcaccct
     3901 ggccacgtcc ctgacccgga gcaatttcgg aatggttcgg atcaccaagg ctccggcggg
     3961 cacggagatc cagatcgccg cgcccatctc ggatctcgag tcccttctcg ccaagatggg
     4021 cggagattcg tcccaggaga gcgtattgac ccttcccaag aacgtgcgac tggtccccgt
     4081 cggtgagccg cgcatggggt tggaaggcga tttttcggga aaaaaaccat gatcagaccg
     4141 aaagccgcag tgcttcccct tctgctggcg ctcggcgcca tgcaagcgag cgcccaggac
     4201 gcggacatcg accccaagga ggcgctccgg cagctcctct ccaatgccct caaggtgacc
     4261 atttccgcgg agatcctgcc caccacggac aagcccgtgt ggaacgcgga gagcaccaac
     4321 ctcacgctgc cgggccgttc catcaaggtg cgcctggagg gagacaacgt ccggatctac
     4381 ctgatctgta cgccgtacgt ccaggatgac ggagtcgtgc tccttcttgc ccagggccag
     4441 gtgtggctgt ccgatcctcc ggacaaggaa tcgaagtact acagcacctt ctacagcatc
     4501 ccggtgacct tcggcaagaa ggtgctgttc ttccccttgg gcgtatcgga cagcgacact
     4561 gccaaggaca acaaggattt cttcaacatc gagctgcaga taaagatcga gcctgccgcc
     4621 cagaagcagt agagagatcc ggatgacccc agagaacgat gctcccggct ccgaagaccg
     4681 ctcgagcgga gactacccgg ggccggacgc ggggcccgaa ggagcggccg agcagcagca
     4741 gaagccctat cagaggattc tccggagcaa gggcttcgtc cccgtcacgg tcctggtcgc
     4801 gctgatcgcg ttcgtccttc tgagctcgcg gctggcggga cggcctcccc agatcgatac
     4861 catcacccca tcccggggaa agcccgggac ggtcctgctg atcagcgggc ggtacttcga
     4921 agcggagcgc ggctcgagcg aggtccgcat ctcgggaatc gctcccacct catcggacta
     4981 cctcgactgg acggatcggc aggtcagcgt gcggatcccg gacggggcca cttccgggat
     5041 cgtctacgtg atcactcgca gcggccgatc gggcggcctg ctctgggtca accaggacca
     5101 gatcccggta cccgcatccg ggccgtcccg tccgggggag ccctatatca aggagatcaa
     5161 gccgtccgcc gctcaggtcg gagacaccat caccatcacc ggcatgaact tcgggctgga
     5221 gaaggacaac tccgaggtgt acttcacgcc ggcggccggc ggagactccg ttgccagcgc
     5281 cgggttcgac tcggtgagca tgctcccggc gaaccagggc gatttcgact acctgtcctg
     5341 gtcggaccgg gagatcaccc tccgcgtgcc ggacggggcc gcctcgggaa acgttgtggt
     5401 gaaatccgac aaggctccca gcaaccccgt gtacttcgag ctcctgggtg gggtcggcac
     5461 caagctgtat ggctcccctc ggaagtactc cgtccagtat gccatggacg cggaggtcag
     5521 cgccgcctcg ggtgagaaca cgctctatct ctggatgccg agcatcatcc agaccccgga
     5581 gcagagacgg tctcacctgg tctccgagga gccccagccc ctggcggtcc gggacggccc
     5641 tcggctctat tccctgggca acgtgcagaa gggaagccag gcgcgggcaa gcctgagctg
     5701 gatcttcgat cgctacacgg tggagaccca ggtgagcctt gccaagccca tccccgccta
     5761 cgacgactcg acggacctct accacacgtt cacgcagccg gacgccctgg tcccgtccaa
     5821 caacccggac ctcatcaagg cagcggcggc cgccaccggc ggagagaaga gcccgtacct
     5881 caaggcgcgc cgcgtctacg actggctgct cggtcaggtg acctactcgg agaccgccag
     5941 caatccgctc caggtcctcc gccagaagcg ggcggatgcg ttcgcgttct cctccgtgct
     6001 ctgcgcgatg ctgcgggcct ccgggattcc ggcgaggatg gtatcgggct acctggtggg
     6061 ggagaccggc cagcccacgc gcaggcactt ctgggacgag ttctacctcc ccacgttcgg
     6121 ctgggtcccg gtggatcccc tcctgggaga cgacccttcc ctgtacccga tgacggtcgc
     6181 cgccgacttc gacgcccacg cctactactt cgggaatctg gacaaccagc acgtcacctt
     6241 ctccaaaggc ctcacctcgg tgaaccagat gagccccatg gggaagacgc gtcagttcag
     6301 cgagttcccg tacctggtat cgatcgatgc ggagacggtg ggcggggtaa cgctgggcgg
     6361 ggtcaccttc gaggatctcg tggtaagcgg aacctactga cgggactgcc gggccggccg
     6421 tcttgcgacg ggcgccgtgg atgcgggctc agacccccgc ggccgccttg cgacgggcgc
     6481 cgtggaagcg ggctcagacc cccgcggccg ctttgcgacg ggcgcgctca atgcgctcca
     6541 tgtgcttgta gtactcggac tgcttgtgga tctcttcgac atccacggtg gcgatcttgt
     6601 tgtcggacag gaccatcttc ttattgtcga aaagcttgcg gacgaacggg gcgcccttct
     6661 cgttggagag gcccacgaag ttcaggagct ccttgggagt gagctggaac gtgtacttgc
     6721 cgcccttgac gatgggaacc cgattccgct ccaggtggag cagcagcgca tcgtagagcc
     6781 tgcccagcgg gtcctggatc agcgtgttgg cgagctgtcg gtacagcgac cacagccgct
     6841 ccgccaggag ggtggtgagg cgggtgatga gctgcgcctg cgtctggacc atcctctcga
     6901 agttgccctt gttcaccgcc atgaggctgc agtcctcgtt ggccagggcg gacgcggatc
     6961 gcggcttgtt ctccagcagg gccatctctc cgaagatgtc gccggccttg agcagggcca
     7021 gcatcacctc gttgtcctcg acgatcttgg tgatcttcac gacgcccttc tggatgatgt
     7081 acaactcgct gccgggctcg ttctcactga agatcatcgt gttcttgggg taggtgcgcg
     7141 tgaaatcgtc cgaggccttc tccagataga ccgccttggc gtacgggtgg atcttggcca
     7201 gccgctcctt ggccttctcc gcgttgggat ccgtggggca gttctggagg aagcggtagt
     7261 aggcgtagta ggcctgattg tactggctct ggcgtgcgta gtactccgcc accttgaaca
     7321 ggtgggacgg gtcgccgctt gccgtgttct tgaaggtgag acgcgtcagc gcctcgtcca
     7381 ggtagcgcat cttgcgggag aagctctgga tgatcttcat ggcgaccgga gcgttgcgtt
     7441 cgatcaggag cccgtactgg tcccgggcca cggagatcaa ggatacgtcg gagagggcga
     7501 gggccgtctc gatatggctg tgggcggaca tcgtggagat gacgccgaag aagtctccgg
     7561 gggccaggat gttcccgccc tcctcctcca cgacctcggt ttccttgctg acccggacct
     7621 ttccgctcct caggatgtag aagcagtccg cattctgctt gccctcgacg atgatgtatg
     7681 agtttttctt gaagtttact atcgaaagct ggagcgggct cgccattcgt tatttccgtt
     7741 gtatccgggt cccagtatag agatcggttt ttgagttgtc aatgtgaaga acggcaatcc
     7801 cctgtgcggc ggttctttgg tagccgtccg cgcggcgctc tggtatagtg gtctctcgat
     7861 gcgcccggcg gatgagcacc tgcgctggac cgagcacgcg aggacgcggc tggcatcctg
     7921 cgccttcttc cagttctact ccagcgagcg gcggtccaac tcgggcaagt ccgcttcgtt
     7981 ctgcctgctc gatgcgcccg attgggtgaa catcgtgccc gtgcttcggg acccctcggg
     8041 ggaggagcgg ttcctgatgg ttcggcagta ccgccacggg gcggatctca tcaccaccga
     8101 gtttcccgcc ggcttgctcg agcctggaga ggatcccgtc gccgccgcgc atcgcgagct
     8161 gcgggaggag accggctacc gcgccggccg catgacgctc ttgggaaggg tttcccccaa
     8221 tccggccttc atgaacaact ggtgcagcac gtacctggcg gaggacctgg ttccggcagg
     8281 cgagccgaac ctcgacgagc ttgaggagct ctcaacggtc acgctctccg cgcgcgctct
     8341 ggaggaaggg atcggtcggg gggagctcgt gaactccatg gtcctggtgg ccctgctctg
     8401 gtatcgcctg gcgaggtccc gatgaggctg cgaatcagca agccggtgaa gatcgccatc
     8461 gaggtggtgg tgatcgggct gctggttctc ctcttcttcc tggtggtgga cccggcccga
     8521 ctgcgggtgt acctggcgct catcacgctg cggtccattg gcggcctcct ggccatccag
     8581 ctcacgctgc acctggtggg catggtccag tggctcctca tcctgcgcca ggccggcgtg
     8641 accagctccg cctgggaggt cttctggacc cgattgagcg gatgcgccat cacctccttg
     8701 acgccgtcga tgtacttcgg cggcgagccg gtccgagcca gcatgctcaa gaaccccacg
     8761 gtgagctatc agacgctcta cgcgaccatc gccgtggaca agtacgtgga gctcttcacc
     8821 aagctgcctg ccgccgccat cgggtttgcc atgctgatcc agcttgcgca gcccggcccg
     8881 gcgctcctga tcctctccgc cgcgttcatc ctggtgttct gcgccttctt cctctacctg
     8941 atggtgaagc tcttccaggg cagccgcttc gtgctgcgcg cggccaagtg ggtgctcgtc
     9001 cccatggccc gggtccgccc ccgcctggcc gtgaagatcc agatggcgct gaaggagttc
     9061 accaaggacg tgtcgcggat cctcaagaag aagctctcct tcgcgctggc catgttcttc
     9121 ggtctgttgt tgagcgcggt ggaggtcctt cagatctact acgtcctggc cgtgttgggg
     9181 gtctacgatc tccgacgggc ctttgccatc ttctttgcga ccgtgttcca gggcctgctg
     9241 agcttcatac cggggaacat cggcaccatg gaagggctca acctgttcat gttcagcatc
     9301 ctgaagctgg gg
//
LOCUS       DASRZS010000672         3975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_753595, whole genome shotgun sequence.
ACCESSION   DASRZS010000672 DASRZS010000000
VERSION     DASRZS010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753595"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VFH83_16365"
     CDS             complement(<1..790)
                     /locus_tag="VFH83_16365"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET6488003.1"
                     /translation="MYDSTDAQAFIRSHTRFVVLGHKEPDGDCVSSQLAVADLLTRLG
                     HQASVYSVGPFDRPEIASLAQRFASQIPTESLEGAAVVIVDCSTPDRTGALGKTVEGL
                     PTLVIDHHSSGEAFGTARFVEPTAPSSSMLALQLYDDLGLTPDSEMARLLLFGLCTDT
                     GFFRHLAQSASETFRAVARLADLGATTAEVFMMVYGRRELAARKLLGESLVHCESHWS
                     DRFLLTWQTLDDRVRLGVTQRGEDDLYRLLQTVRGNVVVALIKEE"
     gene            complement(793..1962)
                     /locus_tag="VFH83_16370"
     CDS             complement(793..1962)
                     /locus_tag="VFH83_16370"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET6488004.1"
                     /translation="MLDIQRLFEAIRDEGLAGWLVCNSHHRDEIADLVLGVTRERTNT
                     RPWVCLLPAAGEPVKVVHAMEAGILDGVPGRVMPYSTRAQFTGALASVLPRGVPLAAN
                     FSVNTPVSSFLDHGTALLVQSLGVTLVSAESLIARALGAIDERGFDSHQAAAAVLYRI
                     VPQVWSRLARQLKDAGSVREGEVRDWIATGIADAGLVSDALPIVAAGSHSGNPHFECV
                     GAGSELKAGDVIQFDLWARQPQEGAVYADISWVGVLGTKPTPAQQETFQTVVAAREEA
                     LRFIEAGLAEGRAVRGEDVDRAARQTIEGRGYSAGLRHRTGHSIGGRVHGFGVNLDSI
                     EFPDRRVLRDGACFSVEPGLYGSEFGMRTEVDCCIRGGRLVVSGAERQRTLLVLA"
     gene            complement(1974..2378)
                     /locus_tag="VFH83_16375"
     CDS             complement(1974..2378)
                     /locus_tag="VFH83_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6488005.1"
                     /translation="MRKTALGLLLLMAVMPATAQQKTYPKDAYVVTEHIVTIALHPLG
                     YRLTYLRTDNTWGNLYVPIAWFGNGPNSKADIGFSAGIEYPFFSIFWVDGAFDHISIH
                     APGNYQDPSWKVIDPSVDLTAQFNVKEPPRDF"
     gene            complement(2518..3450)
                     /gene="argF"
                     /locus_tag="VFH83_16380"
     CDS             complement(2518..3450)
                     /gene="argF"
                     /locus_tag="VFH83_16380"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET6488006.1"
                     /translation="MTMKGRSLLTLKDWTPAEVRFVLDLSRAVKKERRAGKIRDRFRG
                     KTLALLFEKRSTRTRCAFETAFGEEGGHPVFLSTQDIHLGVKEDLEDTARVLGRMFDA
                     IQFRGFKQSTVETLASYSGVPVYNGLTDDWHPTQALADLMTLEEEAGTLTGRKLAFVG
                     DGRNNVANTLLIGCAQMGVHCSIVAPPSLCPSPETLGWARSMGDKTKAVIQVTSDVAE
                     GVSGADAVYTDVWVSMGEEEKSAERIALLSPYQVTTAVMERTGKPDSIFLHCLPAVKG
                     KEVTVEVFEGKRSRVWDQAENRKHTIKALMLATL"
     gene            complement(3447..>3975)
                     /locus_tag="VFH83_16385"
     CDS             complement(3447..>3975)
                     /locus_tag="VFH83_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HET6488007.1"
                     /translation="TVVIINKMDTASPAGIAKVRESIRAINPRALVVDAASPISVDRA
                     ADIRGKRVLVVEDGPTLTHGEMAYGSGTVAAQKHGAGEIVNPRPWATGKIKETFDRYP
                     GIGALLPAVGYGDEQVRDLEATIRRVPCDLVIVATPVDLTRIIRIDKPMLRVRYELQE
                     LGQPSLEEILKARLP"
BASE COUNT          675 a         1352 c         1290 g          658 t
ORIGIN      
        1 cctcttcctt gatcagcgcc accaccacgt tcccgcggac ggtctgcaga agccggtaga
       61 gatcgtcttc tcccctctgg gtgacgccga gccggacccg atcgtccagc gtctgccagg
      121 tcaggagaaa ccgatcggac cagtggcttt cgcaatggac cagggactcc cccagcagct
      181 tgcgcgcggc gagctcccgc cggccataca ccatcatgaa gacttccgcc gtggtcgcgc
      241 ccaggtccgc cagcctggca acggctcgga acgtttccga ggcgctctgc gccaggtggc
      301 ggaagaagcc ggtgtcggta caaaggccga acaggagcag gcgggccatc tcgctatcag
      361 gagtgagacc cagatcgtcg tagagctgga gggccagcat ggaagacgac ggagccgtag
      421 gctccacgaa tcgggccgtc ccgaaagcct ccccggagga gtgatgatcg atcaccaggg
      481 tggggagacc ttccacggtc ttgcccagcg ctccagtgcg gtccggcgtg gagcagtcca
      541 cgatcacgac agcggcgccc tcgaggctct ccgtgggtat ctggctggcg aaccgctggg
      601 caagggacgc gatctcgggc cggtcgaacg gcccgacgga gtacacgctg gcctggtgac
      661 ccaggcgggt cagcaggtcc gcgaccgcaa gctgggagga cacgcagtcg ccgtccggct
      721 ccttgtgccc caggaccacg aaccgggtat ggctgcggat gaaagcctga gcgtctgtgg
      781 aatcgtacac ggctacgcca gcaccagcag ggtccgctgc cgttccgcgc cgctcaccac
      841 gaggcgaccg ccgcggatgc agcagtccac ctcggtacgc attccgaact cggagccgta
      901 caaccccggc tccacggaga aacacgcccc atcccgaagg acccgtcggt ccgggaactc
      961 gatcgaatcg aggttcaccc cgaacccatg cacccggccg ccgatggagt gtccggtccg
     1021 gtgacgcagc ccggccgaat acccgcggcc ctcgatggtc tgacgagcag cgcggtccac
     1081 gtcctctccg cggacggcgc ggccttccgc aagccccgcc tcgatgaacc ggagcgcctc
     1141 ctcacgggcc gcaacgacgg tctggaaggt ctcctgttgc gcgggagtgg gctttgtccc
     1201 caggaccccg acccaggata tgtcggcgta gacggcaccc tcctgtggct gccgggccca
     1261 gaggtcgaac tgtatgacgt cgccggcctt gagctcgctg ccggctccga cacactcgaa
     1321 gtgcgggttg ccggagtgcg atccggccgc aacgatgggg agagcgtcgc tcaccaggcc
     1381 ggcatccgcg atccccgtgg cgatccagtc ccgaacctcg ccctctcgga ccgagcccgc
     1441 gtccttgagc tgccgggcca gccgggacca cacctggggt acgatccgat agagcacggc
     1501 cgcggcggcc tggtgggagt cgaacccgcg ttcgtcgatg gcgcccaggg cgcgggcaat
     1561 cagcgactcc gcggagacca gcgtgacgcc aagggactgg accaggagcg ccgtgccgtg
     1621 gtccaggaaa gaggagaccg gggtgttcac cgagaagttg gcggcaagcg gaacgcctcg
     1681 gggcaggacg gaggccagcg cacccgtgaa ctgggcccgc gtggaatacg gcatcacgcg
     1741 gcccggcacg ccgtccagga tcccggcctc catcgcgtgg accaccttca ccggctcgcc
     1801 ggccgcgggc agcaggcaca cccagggacg cgtgttggtc cgctcccggg ttacccccag
     1861 gaccagatcg gcgatctcat cgcggtggtg gctgttgcat accagccaac cggcgagccc
     1921 ctcgtcccga atggcttcga acaggcgttg gatgtcgagc atgagccggg acgctagaag
     1981 tccctcggcg gctccttgac gttgaactga gcggtcagat cgacgctcgg gtcgatcacc
     2041 ttccagcttg gatcctggta gttccccggt gcgtggatgc tgatgtggtc aaaggcgccg
     2101 tccacccaga agatggagaa gaaaggatac tcgatccccg cgctgaagcc gatgtctgcc
     2161 ttcgagttgg gtccgttgcc gaaccaggcg atgggcacgt agaggttgcc ccaggtattg
     2221 tccgtccgga ggtacgtgag acggtagccc aggggatgga gcgctatcgt gacaatgtgc
     2281 tccgtgacga cataggcgtc cttgggatac gtcttctgct gcgctgtggc gggcatgacc
     2341 gccatcagaa ggagcagccc gagcgcagtc tttctcatcc tgtgcctcct gagccttcgt
     2401 ccgaaccttc tataataata gcaatccgga gcctctttgc aaggttttgc gtgccggcgg
     2461 actggagtgc cgggggactc ggtggggcgg ctccgcgtcc cgagcttccc gcctccgcta
     2521 cagcgtggcc agcatgagag ccttgatggt gtgcttgcgg ttctccgcct ggtcccagac
     2581 ccgcgagcgc ttcccctcga acacctccac ggtcacctct ttacccttca cggccggcag
     2641 acagtgcagg aaaatggagt ccggcttgcc ggtcctctcc atcaccgcgg tcgtgacctg
     2701 gtacggcgac agcagggcaa tccgctccgc gctcttctcc tcctcgccca tggagaccca
     2761 cacatccgtg tagaccgcat cggcgccgct caccccctcg gcgacgtccg aggtaacctg
     2821 gatgacggcc ttcgtcttgt cccccatgga ccgggcccag cccaaggtct ccggggaggg
     2881 acagagagac ggcggcgcca cgatggagca atggacgccc atctgggcgc agccgatcag
     2941 gagggtgttg gcaacgttgt tcctgccatc cccgacaaat gccagcttgc ggccggtcag
     3001 cgtccccgcc tcctcctcca gcgtcatcag gtccgcaagg gcctgggtgg gatgccagtc
     3061 gtcagtcagg ccgttgtaca ccggcacgcc ggagtagctg gccagcgtct ccacggtgga
     3121 ttgcttgaac ccgcggaact ggatggcgtc gaacattcgg ccgaggacgc gggcggtatc
     3181 ttccaggtcc tccttgaccc ccaggtggat gtcctgggtg gagaggaata ccggatgccc
     3241 tccctcctcg ccgaaagcgg tctcgaacgc gcaccgggtc cgcgtggagc gcttctcgaa
     3301 caggagcgcc agggtcttgc cccggaaccg atcccggatc ttgccggccc gccgctcctt
     3361 cttcaccgcg cgggagaggt ccagcacgaa gcgcacctcg gccggagtcc aatccttcag
     3421 ggtcagaagc gacctcccct tcatggtcat ggcaacctcg ctttcaggat ctcctccagg
     3481 ctcggctggc ccagctcctg gagctcgtaa cgcacgcgca acatgggctt gtcgatccgg
     3541 atgatccggg tcagatccac cggcgtagcc acgatcacca gatcgcacgg aacgcggcgg
     3601 atggtggctt ccaggtcccg gacctgctcg tccccgtagc cgaccgctgg caggagcgcg
     3661 ccgatgccgg gataccgatc gaaggtctcc ttgatcttgc cggtcgccca gggacgtgga
     3721 ttgacgatct ctcccgcccc gtgtttctgc gccgctaccg tgccggaccc ataggccatt
     3781 tctccgtggg tcagcgtggg accgtcctcc accaccagca cgcgcttgcc gcggatatct
     3841 gcggcgcggt ccacggagat gggggaggcg gcatccacca ccagggcccg gggattgatg
     3901 gcgcggatgc tctcccgaac ctttgcgatc ccggccgggg aagccgtgtc catcttgttg
     3961 atgatcacga cggtg
//
LOCUS       DASRZS010000673        12765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3845987, whole genome shotgun sequence.
ACCESSION   DASRZS010000673 DASRZS010000000
VERSION     DASRZS010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 12765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12765
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3845987"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1064)
                     /locus_tag="VFH83_16390"
     CDS             complement(168..1064)
                     /locus_tag="VFH83_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6488008.1"
                     /translation="MTTTQLLLQQLINAVSIGSLYALMAIGLAMVFGILRLINFAHGD
                     LIMVGSYIALFAMILGLPFAAAAVIGVAASTVAGLVMERVAYRPLRGAHEISMLLTSF
                     SVTIFLENLAIVLFRPTSRPFPAPGLLRAVLSVGQLRVASLDLIAIAVAGLLLLALTL
                     FVRRTDIGIAMRAASQDLLAARLMGINVNAVIASAFAIGSLLAGVVGLLWSARVGKVD
                     PLMGFNPVLKAFVASVIGGLGSLPGAVIGAYVLGALEILLQGLLPAALTPYRDAFVFA
                     ILIALLLFRPNGILGATEREKI"
     gene            complement(1061..1783)
                     /locus_tag="VFH83_16395"
     CDS             complement(1061..1783)
                     /locus_tag="VFH83_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6488009.1"
                     /translation="MLKLESVDVAYGEVLALCGVTLEVRDGELVTLLGANGAGKSTTL
                     MTISGIQRLRAGSIQYDGKDLTRASAFDIVGMGIIQCPEGRRVFGGLSVLENLRMGMI
                     RRRDKASARADLDWVFSLFPVLGERRHQSGATLSGGEQQMLAIGRALMAAPRLLMLDE
                     PSLGLAPLVVRSIFRVIGELHRRGVTILLVEQNVHQALAVADRGYVLASGKVMLHGTG
                     AELRAHPELEQAYLSAVGEARP"
     gene            complement(1761..2576)
                     /locus_tag="VFH83_16400"
     CDS             complement(1761..2576)
                     /locus_tag="VFH83_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6488010.1"
                     /translation="MQTAGAQNEGVLLEIDALSKTFRGLHAVDGYHVSVRPGEILGII
                     GPNGAGKSTVFNLLTGHLRASSGIIRFRGTDITGLPPDRIARLGIGRTFQNIRLFPSM
                     SVLENVVAARQLRQRSSFLSTLVSWPSFQRTERELASHATEALSLFGLSGRTSVPATA
                     LPYGDQRRLEIVRALALEPVLLLLDEPTAGMNAAESMQILDLIRQIRGRYHLTIILVE
                     HNMPLVMTLCERIQVLNYGRLIAEGTPEEVRKDPKVVESYLGQTAEDAQARIG"
     gene            complement(2649..3686)
                     /locus_tag="VFH83_16405"
     CDS             complement(2649..3686)
                     /locus_tag="VFH83_16405"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET6488011.1"
                     /translation="MNRVGLLRDPLFLLHSDDLDHVEIPDRLRAIEAMLEGSPFRDRL
                     VELPARDATREELSWVHTEKHIRAVEGTRGGDLVWLEAETSANGHSHAAAVRAAGGVI
                     SCVDAVVAGGLTAAFALVRPPGHHAEAGQAMGFCLFNNVAIAAEYALRRLGVRRVLIY
                     DWDVHHGNGTMHSFYGTDRVLFVSTHQYPNYPGTGAIDEVGSGPGAGYTVNVPLPPGQ
                     GDREFAEVMDRVLAPLARGFRPQLILISAGFDIAQGDPLADMTVSPAGFARMTQAVME
                     IADAFCPGRLVFTLEGGYNLGALASGVSAVLSTLADGKGKAAPVDGDCKQETTAVIDG
                     LLKALRPYWAI"
     gene            3762..4874
                     /locus_tag="VFH83_16410"
     CDS             3762..4874
                     /locus_tag="VFH83_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6488012.1"
                     /translation="MVPEALNVPLDSDHWEWGGPPPVLGEHRGEKCLCFPESFTVAAA
                     RNLSLTDGVIQARVSVPRARSFHGLVWRCQDRENYESFFVRPHQVGNPDSIQYTPVFN
                     DVSCWQLYHGEGFWAPILFPIDEWFVIRVAFAGQAAEVYVEDLARPVLRCELKASRAP
                     GRVGILCSGPGLRVAGFSAGPSADPTDPAVQAVPRPHPQPDAGAIRSWWISSTVPEAE
                     IAGVSTLAPGAWRELTWDRLDAEPSGLANIARVRRIEERRNTVFATAVIRSSRQQRKA
                     LAFGFSDRATVFLNGRALFRGEDAFRSRDYRFLGSVGYWDTVYLPLDKGENQLVIAVS
                     EDFGGWGVQARFADREGIELPDQHDASGTPVWNGVD"
     gene            4896..5513
                     /locus_tag="VFH83_16415"
     CDS             4896..5513
                     /locus_tag="VFH83_16415"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HET6488013.1"
                     /translation="MIRRALFLCGIVAAVLFVVTVVVGGVLRPGYDHLSMAVSELIQA
                     GASNKTLLDALFAVYNVLLLAFAWAAGMSLRGEGRGLISAGAFVLGAVGLLGLAMTLF
                     FPMNPRGAPATTAGILHLILAGALSLGTIVSIAFFTFGSKDHGPYWIYCLATGVLVLV
                     SGAVAAATAATASPYLGLAERTTIGLFLQWLAVFSVRLVREDLGM"
     gene            complement(5532..6455)
                     /locus_tag="VFH83_16420"
     CDS             complement(5532..6455)
                     /locus_tag="VFH83_16420"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET6488014.1"
                     /translation="MTIRDELVRVSSDRLRRDLFHISRDPLPFRAVNQRRPGQTRHTL
                     EETDDFLRGELAAAGYAVEATTHRVQAFRCDRSKPLHHWYSAPDPADAFYEIANLEVT
                     REGTKLPDQIIQLVSHKDSMSWIDSPGAHDNGVGTVANLELARAVADLPLRRSVRILF
                     CNEEHTPWTSRFAAEAAARRGDRIVAVLNQDSLDGKSDDEMAAGKLTHVVCWSTEEGR
                     ELAELVAGCARRYELPLETRVARKDQINDDDGMYINAGYRRTVMSLGSWPYADSQYHM
                     PGDTPDRVSIQNLTLSTMLILASVLELDAAP"
     gene            6567..6956
                     /locus_tag="VFH83_16425"
     CDS             6567..6956
                     /locus_tag="VFH83_16425"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HET6488015.1"
                     /translation="MTSRTLAMIVLGAVVMLSSGCTSAEMTATRQLPITRVDLSQARD
                     GSYDGEYTYGRFTYRVEVVILDHRIVTIRILDNRNTRRALMAEGVVDRILQSQRNDVD
                     AVSGATTTSKALLKATENALTRSLASP"
     gene            7057..7593
                     /locus_tag="VFH83_16430"
     CDS             7057..7593
                     /locus_tag="VFH83_16430"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6488016.1"
                     /translation="MTVTLMRHYRVNIRWESRYTAQSYREAMRSYDNADVLDQRLEPD
                     GEYQHVITSALKRSRQTQALLCGRRPSLQTELLNEVPLEPFTRDRRERSLQLWNTMAR
                     LQWLVNDPKQPETRRGTRARAREFVDAFLRDEGSYLVISHGFFLRILSRELRKRGFRG
                     RAIQFMRNGERRTYLRTG"
     gene            complement(7610..9709)
                     /locus_tag="VFH83_16435"
     CDS             complement(7610..9709)
                     /locus_tag="VFH83_16435"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HET6488017.1"
                     /translation="MATFLLQNETLRLELDRDTGALTGVTAPKTGWRILDRPALGLSF
                     RLLVPLSEELRNNPVYGEKQRLAAPAELSADGRGATLVWDDLASERGGRLPIRLTLRI
                     SLDDRRAVFTVSIENRSKYVVENVYCPYLGDVQHPPNAEWLKTFLYNYASAQEWSMWP
                     KFLNMRGYYGVDYPTQVSAWGASSGAPMSPYILLRSADQGLYVGVSAPSAELVAWHAE
                     LRPGYGSSIDARVPEVPVIAGKDVATRFAAVHVPYLQPGESRSLTPVALEAFTGSWHA
                     GVDIYKRWRSGWMKLPQVPAWAREPHAWQQVHINSPEDELRVRFTDLPRIGEACARHG
                     VKAIQLVGWNDGGQDQGNPSHDPDPRLGTFQELKDAIARIHAMGVRVILFAKFTWADR
                     ATERFRKELAPLAIKDPYGDYQMHGGYQYQTATQLLDINTKRLVPMCFLSEEYLRLCD
                     REFQKTLDLGAAGILFDECLHHSPALLCFDPAHGHRLGAPVYANDRLLIRNFHRLSDG
                     VKTDFLYAGEACYDWELEAYHLSYHRSESKEHIPLSRYMLPEAQLMTAVTGFDDRAMI
                     NQCLMYRYIVSYEPFNFKGSLDDFPATVAYGRKMDALRTELREYFWDGEFRDEAGAAV
                     TADGKPHRPYAVFVSRTTGRAGIVICNYDEHQSVTVQARWTDTGAPGRYRTVDDPTWQ
                     PCAAGIRIPPQSAVVAI"
     gene            complement(9804..10895)
                     /locus_tag="VFH83_16440"
     CDS             complement(9804..10895)
                     /locus_tag="VFH83_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HET6488018.1"
                     /translation="MKVTSRPVIRDISVRPFDIPLKGALRIATMGMETAQNLLVKVVT
                     NDGVQGWGEASPFHSITGETQAIDLAAAAELRELLVGRNPLAIAEIAELMRRHLPHTP
                     TIRSAFDMALYDIASRYASMPLYRFLGGESRPLATDITIYLGDAERSAELARQIVREG
                     FHAVKVKLGQDRTGDLARVRAVRGASAEAVIRVDANQAWDRITALDMLRALAEHSIEF
                     CEQPCAASDHRTLRHLSAHSPIPIMADESVFSPADALALDREDAVPYFNIKLSKSGGI
                     GPARQIAGIAEAGGRVCMLGCMSESRLGITAAAHVALAHPIIRFYDLDSFWEHREDPI
                     QGGVTIHDGCLVVPEEPGLGATPDESYLA"
     gene            complement(10892..12544)
                     /locus_tag="VFH83_16445"
     CDS             complement(10892..12544)
                     /locus_tag="VFH83_16445"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET6488019.1"
                     /translation="MTMWYGFLGGTVDTAGICRLIAEETRRHSIPLLDVALVGAEGTV
                     WHRRFSQGREESAETAAVPVVYRAGSISKMLTAVAVMRAVESGGLDLDAPIHACCPEL
                     VFADPSGAPAAVTLRHLLSHTAGLQRESPVGSYFDSTGPAIEETVLSMVGTHLVLAPG
                     TRAKYSNIGPTIAAYVLSRVTGIPWGRQIEEGILVPLGMRHSGFTVRHTPPGARASDA
                     WMTAFDGSMFPAPRFDLGTPPSGNLCSTAEDLARFARCLLAGGTAGDYRMLRPETLRA
                     MATVQPVRGDPAPSAGFGLGMAVGTLHGCRTVGHGGAVYGYSADLLLLIDEGVGAIVL
                     NNLDCAGGVNAKIRTRLLETALGERGRSFEPVFGKGDPGPVEKLPGYAGSYRSADEWA
                     EVTVGSGQLVLHSVGTSKRLRRVGTDRFVTDDRMSAGVSVEFLRDEVGRVSELRAGET
                     SFVRAEPAAPAADPDLGGFCGDYGPPHAGLRILRRASRLVCRIEWMFEYDLEPLGDSR
                     FAFRGHGMYEGEELRFGWDDHGRAAEVRLSGIRFPRREGAQE"
     gene            12543..>12765
                     /locus_tag="VFH83_16450"
     CDS             12543..>12765
                     /locus_tag="VFH83_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6488020.1"
                     /translation="MGCAKGPDERYARLVTPSGTPSAVPSATPSVGSSVTVEEVPSAS
                     RAHLVSLLDLSFDGIYRWHARRTLPQVRWV"
BASE COUNT         2147 a         4292 c         4298 g         2028 t
ORIGIN      
        1 cccggtgaag ccgttggaga ggttgagggc caccaccagg atgacgttga tcccgatgat
       61 gatgacgatc cgctggacgt agtctccggc caggaacgcg cagagggcga gcaccacggc
      121 aagcagcgcc aggagaacgg caggcccgaa cagccgggag cgcggcatca gatcttctcc
      181 cgttccgtgg cgcccaggat tccgttggga cggaagagca gcagcgcgat caggatggcg
      241 aacacgaacg catcccggta cggagtcagc gcggccggca gcagtccctg gaggaggatc
      301 tccagcgcgc cgaggacata ggcgccgatc acggccccgg ggaggctgcc caacccgccg
      361 atcacggagg ccacgaatgc cttgagcacc ggattgaagc ccatgagcgg gtccaccttg
      421 cctacccgcg cgctccaaag gagcccgacc acccccgcca ggagggaacc gatggcgaac
      481 gcggaggcga tgaccgcgtt gacgttgatg cccatgagcc gggcggcaag aaggtcctgg
      541 gaggccgcgc gcatggcgat gccgatgtcc gttcgacgca cgaagagggt gagggccaga
      601 aggagcaggc cggcaacggc gatggcgatg agatccaggc tggccacccg gagctggcca
      661 acggacagga ccgcccgcag gagacccggc gcggggaacg gacgggaggt gggccggaag
      721 agaacgatgg ccaggttctc cagaaagatc gtgacggaga acgaggtcag gagcatcgag
      781 atctcgtggg cgccgcgcaa tggccgatag gcgacgcgct ccatgaccag ccccgccacg
      841 gtgctggcgg ccaccccgat cacggcggcg gcagcgaagg gtagacccag gatcatcgcg
      901 aacagcgcga tgtagctgcc caccatgatg agatcgccat gggcgaagtt gatgagccgc
      961 aggattccga agaccatggc caggcctatg gccatcaggg cgtagaggct cccgatgctc
     1021 accgcgttga tgagctgctg gaggagaagc tgcgtggtgg tcacggccgg gcctccccca
     1081 cggcgctgag gtaggcctgc tccagctcgg gatgcgcgcg gagctcggcg cccgttccgt
     1141 ggagcatgac cttgccgctg gcgagcacgt agccacgatc ggcgacggca agggcctggt
     1201 gcacgttctg ttccaccagg aggatggtca ccccgcggcg atgcagctct ccgatcacgc
     1261 gaaagatcga ccgcaccacg aggggagcca ggcccaggga cggttcatcg agcatcagga
     1321 ggcgaggggc ggccatcaag gcccggccga tcgccagcat ctgctgctcg ccgccggaga
     1381 gcgtggctcc gctctgatgc cgacgctccc caaggaccgg gaacaggctg aacacccagt
     1441 ccaggtccgc acgggcggag gccttgtccc gcctgcggat catccccatg cgcaggttct
     1501 ccagcacgga gaggccgccg aagacccggc gaccctcggg acactggatg atccccatac
     1561 cgacgatgtc aaaggcgctg gcccgggtca ggtccttccc atcgtactgg atcgagccgg
     1621 cgcgcagccg ctggatcccc gagatcgtca tgagggtggt agacttgccg gccccgttgg
     1681 cgcccaggag cgtcaccagc tcaccgtcac gcacctccag ggtcacgccg cagagcgcga
     1741 gcacctcgcc gtaggccacg tcaaccgatt cgagcttgag catcttcggc cgtctgaccc
     1801 aggtatgatt ccacgacctt cggatccttg cgcacctcct cgggagtccc ttccgcgatc
     1861 agccttccgt agttcaggac ctggatccgc tcgcacagcg tcatgaccag gggcatgttg
     1921 tgctccacca ggatgatcgt gaggtgatag cggccccgga tctgacggat gaggtcgagg
     1981 atctgcatgc tttccgcggc gttcatcccg gcggtgggct cgtcgaggag gaggagcacc
     2041 ggctcgagcg ccagggcgcg gacgatctcc agcctccgct gatccccata gggcagcgcg
     2101 gtggcgggca cggaggtccg accggacagc ccgaacagag agagggcctc cgtggcatgg
     2161 gatgccagct cccgttccgt gcgctggaac gacggccacg agaccagcgt ggagagaaaa
     2221 gagctgcgct ggcgaagctg tcgcgccgcc accacatttt ccaataccga catgctgggg
     2281 aagaggcgga tgttctggaa ggtcctgccg atgcccagcc gggcaatgcg atccgggggc
     2341 aggcccgtga tgtcggtgcc ccgaaaccgg atgatgccgc tggaagcgcg caggtggccg
     2401 gtgagcaggt tgaacacggt ggacttcccg gcgccgttgg gcccgatgat ccccaggatc
     2461 tccccggggc gcacggacac gtgatagccg tccaccgcat gaagaccacg gaaggtcttc
     2521 gacaaggcgt ctatctcgag caggaccccc tcattctgag ctcccgccgt ctgcatggtg
     2581 tgcccgtggt atagcagaaa acaggcgcca cggctatatc ggacggtgcc gccatgccga
     2641 cccggtggct agatcgccca atacgggcgc agggctttca gcaggccgtc gatgacggcg
     2701 gtggtctcct gcttgcagtc accgtccacg ggcgcggcct tgcccttgcc gtccgccagg
     2761 gtcgacagca ccgcggacac gccgctcgcc agagctccga ggttgtagcc gccttccagg
     2821 gtgaagacga gccggccggg acagaaggcg tcggcgatct ccatcaccgc ctgggtcatc
     2881 cgggcgaacc cggcggggct caccgtcatg tccgcgaggg gatccccttg ggcgatgtcg
     2941 aatccggccg agatcaggat gagctgcggc cggaagcccc gagcaagcgg cgcgagcacg
     3001 cggtccatga cctccgcgaa ctcccggtca ccctggcccg gcggcagcgg aacgttgacg
     3061 gtgtacccgg cgcccggccc ggagcccacc tcatcgatcg cgccggtgcc cggatagttg
     3121 ggatactgat gggtggatac gaaaaggacg cgatccgttc catagaagga atgcatcgtt
     3181 ccgttcccgt ggtgcacatc ccaatcgtag atgagaacgc gccgcacccc gagccgccgc
     3241 agtgcgtact cggccgcgat cgcgacgttg ttgaagagac agaagcccat cgcctgaccc
     3301 gcttcggcgt gatggccggg cggccgcacc agggcgaagg ccgcggtcag accgccggca
     3361 accacggcgt ccacgcagga aatgaccccg ccggcggcgc ggaccgccgc cgcatgggag
     3421 tgtccgttcg cggaggtctc cgcctccagc cacaccaggt cccctccccg tgtcccctcg
     3481 acggcacgga tgtgtttctc ggtgtggacc caggagagct cctctcgggt tgcatcccga
     3541 gccggtagct cgaccaggcg atcccgaaac ggagaccctt cgagcatcgc ctcgatagcc
     3601 ctcagacggt cggggatctc gacatgatcc aggtcatcgg agtggaggag gaacagggga
     3661 tcgcggagaa ggcctacgcg gttcacccga ggacggtagc ggaaaccgcg cgcatgcgcc
     3721 attgccttcc cgatggaggc ctcggagcat acctttcctc catggtgccg gaagcgctga
     3781 atgtcccgtt ggactccgac cactgggaat ggggagggcc accaccggtc ctgggagagc
     3841 accgcggaga gaagtgcctc tgcttccccg aatcgttcac ggtcgccgcg gcccgcaacc
     3901 tgagcctgac cgatggcgtg atccaggccc gggtgtccgt tccaagggcg cggagctttc
     3961 acggtctggt ctggcgctgc caggatcggg agaactatga atcctttttc gtgcgcccgc
     4021 accaggtggg gaacccggac tccatccagt acacgccggt gttcaacgac gtctcctgct
     4081 ggcagcttta ccacggggaa gggttctggg cgcccatcct gttccccatc gacgagtggt
     4141 tcgtcattcg agtcgccttt gccggccagg cggccgaggt ctacgtcgaa gacctggccc
     4201 ggcccgtcct gcgatgcgag ctcaaggcct cccgcgcgcc gggccgggtc ggcatcctgt
     4261 gctcgggccc ggggctgcgc gtggccggat tttccgcggg tccatccgcc gaccccaccg
     4321 accccgccgt ccaggccgtc ccgcggccgc acccgcagcc tgacgccggc gcaatccggt
     4381 cctggtggat ctccagcacg gtacccgagg cggagatcgc cggggtctcg actctggcgc
     4441 ccggtgcatg gcgcgagctc acctgggatc gactcgacgc ggagccgtcc ggccttgcca
     4501 acatcgcccg ggtccgacgg atcgaagagc ggcgcaatac cgtcttcgcc accgccgtga
     4561 tccgttcgag ccggcagcag cggaaggcgc tggccttcgg cttcagcgac cgggcaacgg
     4621 tgtttctcaa cggccgggcg ctcttccgcg gcgaggatgc ctttcggtcc cgcgactacc
     4681 ggttcctggg cagcgtcggc tactgggata cggtctacct tccgctggac aaaggagaga
     4741 accagctcgt gatcgccgtg tccgaggact tcggcgggtg gggcgtccag gcccggttcg
     4801 ccgaccggga aggcatcgag ttgcccgacc agcacgatgc ttctggcacc ccggtatgga
     4861 acggggtaga ctgagaggca tcgaggaggt ggccgatgat ccggcgtgcg ctgttcctgt
     4921 gcggcatcgt ggcagcggtt ctctttgtcg tgaccgtggt ggtgggagga gtccttcggc
     4981 cgggttacga ccatctgtcg atggcggtga gcgagctgat ccaggccggg gcttcgaaca
     5041 agaccctgct cgatgcgctg ttcgccgtct acaacgtcct tctcctggcg ttcgcctggg
     5101 cggcggggat gagcctccgg ggagagggcc gcggactgat cagcgcgggc gcgttcgtcc
     5161 tcggggcggt cgggctcctg ggtctggcca tgacgctgtt cttccccatg aatccccgcg
     5221 gggcgccggc cacgacggcc gggatcctcc atctcatcct tgccggcgcg ctatccctgg
     5281 gcaccatcgt gtccatcgcc ttcttcacct tcgggtccaa ggatcacggg ccgtactgga
     5341 tctactgcct ggcaaccggc gtgctcgtcc tcgtatccgg cgccgttgcc gcagcgaccg
     5401 ctgccacggc cagtccttac ctgggcctgg cggaacggac cacgatcggc ctcttcctcc
     5461 agtggctcgc ggtcttctcg gtcaggctcg tgagagagga cctggggatg tagcggcaga
     5521 ggggcagggc gctagggtgc ggcgtccagc tccaggaccg aagccaggat cagcatggtg
     5581 ctcagggtga ggttctggat gctcacccgg tctggcgtgt caccgggcat gtggtactgg
     5641 ctgtccgcgt acggccagga gcccaggctc atgacggtgc gtcggtagcc ggcgttgatg
     5701 tacatgccgt cgtcgtcgtt gatctgatcc ttccgcgcca cgcgggtctc cagcgggagc
     5761 tcgtatcgcc gcgcgcatcc ggccaccagc tccgcaagct cccgcccctc ctccgtgctc
     5821 cagcacacca cgtgggtcag cttccccgcc gccatctcgt cgtcagactt gccgtccagg
     5881 gagtcctggt tcaggaccgc cacgatccga tcgccgcgcc tcgccgccgc ctccgccgcg
     5941 aacctgctgg tccacggtgt atgctcctcg ttgcagaaga ggattcgcac ggaccgacgg
     6001 agcgggagat ccgccacggc gcgagccagc tccagattcg ccaccgtgcc gacgccattg
     6061 tcgtgcgccc cgggcgaatc gatccagctc atggagtcct tgtgggagac gagctggatg
     6121 atctggtccg gcagcttcgt cccctcccgc gtgacctcca gattcgcgat ctcgtagaag
     6181 gcgtcggcgg gatccggtgc ggagtaccag tggtgcagag gcttgctgcg gtcacagcgg
     6241 aacgcctgga cgcgatgggt cgtcgcctct acggcgtagc cggcggctgc aagctcgcct
     6301 cggaggaagt cgtccgtctc ctccagggta tggcgcgtct gcccgggccg ccgctggttc
     6361 acggcgcgaa acggcagcgg atcccgggag atgtggaaaa ggtcccgccg gaggcggtcg
     6421 gaggagacgc ggaccagctc gtcgcggatc gtcatgaagc catggtgcgg attgcggagc
     6481 gggagggcaa ggggtgcggg tgcagatcga ggcgggcccg gtgttccaca atggtgcggg
     6541 gcccggtggc ccggaggaat ggggaaatga caagccgaac gctcgcgatg atcgtcctcg
     6601 gcgcggtggt gatgctctca agcggctgca cgtcggcgga aatgaccgct acccggcagc
     6661 tcccgattac ccgcgtcgac ctttctcagg cccgggacgg aagctacgac ggtgaataca
     6721 cctacggacg gttcacctac agggtggagg tcgtcatcct ggatcaccgg atcgtcacaa
     6781 tccggatcct ggacaaccgc aacacccggc gggcgctcat ggcggagggc gtcgtcgatc
     6841 gcatcctcca gtcccagagg aacgacgtgg acgccgtgtc cggggccaca acgacgagca
     6901 aagcgctcct gaaggctacc gagaacgccc tcactcggag cctggcttcg ccctaacggc
     6961 gccgcgacgc gccgaggctc ggcgtcccgg ggatcgcgcc ttctgacagg tctccgccct
     7021 ttccttcctc gcgcctccct gagtacgatg gaaccgatga ccgtcacgct catgaggcac
     7081 tatcgggtga acatccggtg ggagagccgc tacacggccc agtcgtatcg cgaggccatg
     7141 cggagctacg acaacgcgga cgtgctggac cagcggctcg agccggacgg cgaataccag
     7201 cacgtgatca ccagtgccct caagcgcagc cgccagacgc aggcgttgct ctgcggccgt
     7261 cggccttcgc tccagaccga gctcttgaac gaggtccccc tggagccctt cacgcgggat
     7321 cgccgggagc gcagcctcca gctctggaac accatggcgc ggctccagtg gctggtgaat
     7381 gacccgaagc agcccgagac gcggcggggc acccgggccc gcgcgcggga gttcgtcgac
     7441 gccttccttc gagacgaagg gagctacctc gtgatcagcc acgggttctt cctgcggatc
     7501 ctttcccgcg agctccggaa gcgaggcttt cggggcagag ccatccagtt catgaggaac
     7561 ggagagcgtc gaacgtacct tcgaaccggc tgagccccgg gagctcctcc tagatcgcca
     7621 ccacggcgga ctgggggggg atccggatgc cggccgcgca gggttgccag gtcgggtcgt
     7681 ccacggtgcg gtagcggccg ggggcgccgg tgtcggtcca ccgcgcctgc acggtcacgc
     7741 tctggtgctc gtcgtagttg cagatcacga tccccgcgcg gccggtggtc cggctcacaa
     7801 agacggcgta cggccgatgg ggcttcccgt cagcggtcac cgcggcgcct gcctcatctc
     7861 gaaactcgcc gtcccagaag tactcccgaa gctcggtccg cagcgcgtcc atcttccgac
     7921 cataagcgac cgttgccggg aaatcgtcca ggctgccttt gaagttgaac ggctcgtagc
     7981 tgacgatgta gcggtacatc aggcactggt tgatcatggc gcgatcgtcg aagccggtga
     8041 ccgccgtcat gagctgcgct tccggcagca tgtagcggga gagcgggatg tgctccttgc
     8101 tctcgcttcg gtgatacgag aggtggtagg cctccagctc ccagtcgtag caggcttccc
     8161 cggcgtagag gaagtccgtc ttcacgccat cggagagccg atggaagttg cggatcagga
     8221 gccggtcgtt ggcgtagacg ggggcgccca ggcgatgccc gtgtgccgga tcgaagcaca
     8281 gcagcgccgg gctgtggtgc agacactcgt cgaagaggat gccggcggcg cccagatcca
     8341 gcgtcttctg gaactcccga tcgcaaagcc gcaggtactc ctcgctcagg aagcacatgg
     8401 gtaccaggcg cttggtgttg atgtccagga gctgtgtcgc ggtctggtac tggtagccgc
     8461 cgtgcatctg gtagtcgcca tagggatcct tgatggccag cggcgccagc tccttgcgga
     8521 aacgctccgt ggcgcgatcg gcccaggtga acttggcaaa gaggatcacc cggacaccca
     8581 tggcatggat cctggcaatc gcgtccttca gctcctggaa ggtgccaagc cggggatcgg
     8641 gatcgtgcga ggggttgccc tggtcctgac ccccatcgtt ccagccgacg agctggatag
     8701 ccttgacccc atggcgcgcg caggcctccc cgatccgcgg aaggtccgtg aaccggaccc
     8761 gaagctcgtc ctccggcgaa ttgatgtgga cctgttgcca cgcgtgcggc tcccgcgccc
     8821 aggcggggac ctgaggcagc ttcatccagc ccgaacgcca gcgcttgtag atgtccaccc
     8881 ccgcgtgcca gcttcccgtg aaggcctcca gggccaccgg agtcaggctg cgggactcgc
     8941 cgggctggag gtatggaacg tgcaccgccg cgaaccgggt ggccacgtcc ttgcccgcga
     9001 tgaccggcac ctccggcacg cgggcatcga tggagctgcc gtacccgggc cgaagctccg
     9061 cgtgccaggc gaccagctcg gcgctcggcg cgctcacccc gacgtagagt ccctggtccg
     9121 cggaacgcag aaggatgtag ggagacatcg gcgcgccgct ggaagcgccc cacgcggaaa
     9181 cctgcgtcgg gtagtccacg ccgtagtagc cgcgcatgtt caggaacttg ggccacatcg
     9241 accattcctg ggccgaggcg tagttgtaga ggaacgtctt cagccactcc gcgttcgggg
     9301 ggtgctgcac gtcgccgagg tagggacagt acacgttttc caccacgtac ttcgaacgat
     9361 tctcgatcga caccgtgaat acggcgcgcc gatcatccag ggagatccgt agggtcagcc
     9421 ggatagggag ccggcctccg cgctcggatg ccagatcgtc ccacaccagg gtggcgccgc
     9481 ggccgtcggc gctcagctcc gctggagcgg cgagcctctg cttctcgccg tagacggggt
     9541 tgttccggag ctcctccgag agaggcacga ggagccggaa ggacagccca agggccggtc
     9601 gatccagaat ccgccagccg gtcttgggag ccgtcacgcc ggtcagcgcc ccggtgtccc
     9661 ggtcaagctc cagcctcagg gtctcattct gcaacaggaa ggtcgccatg gttccgttcc
     9721 tccgcgtcgg gattctgcgc cgacccgggg cgttgggcaa gcgtccggag aaaccgcggc
     9781 ctccccgccg accgaaggcc gtgctacgcg aggtaggatt cgtccggcgt cgcgccgagg
     9841 ccgggctcct cgggaaccac caggcagccg tcgtggatcg tgaccccgcc ctggattggg
     9901 tcctcccgat gctcccagaa ggagtccagg tcgtagaacc ggatgatggg gtgagccagc
     9961 gcaacgtgcg cagcggcggt gattcccaaa cgggactccg acatgcaccc cagcatgcag
    10021 acccggcccc cggcttcggc gatgcccgcg atctgccgcg ccggaccgat gccgcctgac
    10081 ttgctcagct tgatgttgaa gtaaggcacg gcgtcctcac ggtcgagggc cagcgcgtcc
    10141 gcgggactga acaccgactc atccgccatg atgggaatgg ggctgtgcgc ggacagatgt
    10201 ctcagggtcc ggtgatccga ggcggcgcaa ggctgctcgc agaactcgat ggaatgctcc
    10261 gcgagggccc gcagcatgtc cagcgccgtg atgcggtccc atgcctggtt ggcatccacc
    10321 cggatgacgg cctctgccga cgccccacgg accgcccgga ccctggccag gtccccggtc
    10381 cggtcctgcc ccagcttcac tttcaccgca tggaatccct cgcgcacgat ctggcgcgcc
    10441 agctccgcgg atctctcggc atccccgaga tagatcgtga tgtccgtggc cagaggccga
    10501 ctctcgccgc ccaggaaccg atacaggggc atggaagcgt agcgggaagc gatgtcatac
    10561 agggccatgt cgaaggcgct tcggatcgtc ggggtatggg gcaggtggcg ccgcatcagc
    10621 tccgcgatct cggcgatcgc cagggggttc ctgcccacca ggagctcccg cagctcggcg
    10681 gcggcggcaa gatcgatggc ctgcgtctcc ccggtgatgg agtggaaggg agacgcctcg
    10741 ccccagccct gcacgccatc gttggtgacc accttcacca gcaggttctg cgccgtctcc
    10801 attcccatgg tcgcgatgcg gagcgctcct ttcagaggga tgtcgaaagg tctgacggag
    10861 atgtcccgta tgaccggccg gctcgtaacc ttcactcctg tgctccctcc ctgcgcggga
    10921 agcggattcc ggagagccgc acctccgcgg ctcggccgtg gtcatcccac ccgaaacgca
    10981 gctcttctcc ctcgtacata ccgtggcccc ggaaggcgaa ccgggagtcc cccaggggtt
    11041 cgaggtcgta ttcgaacatc cactcgatcc ggcagaccag tcgagaggct ctccgtagaa
    11101 tccgaaggcc cgcgtggggc gggccgtagt cgccgcagaa gcctcccagg tcagggtcgg
    11161 cagccggagc cgcgggttcg gcccggacga agctcgtttc cccagcccga agctccgaga
    11221 cgcgtcccac ttcgtctctc agaaactcga ccgaaacccc cgcgctcatc cgatcgtccg
    11281 tgacgaatcg gtcggtgccg acgcgccgaa gccgtttcga ggtccctacg gaatggagga
    11341 cgagctggcc gctgccgacg gtcacctcgg cccactcgtc cgcggaccgg tagctgccgg
    11401 cataccccgg gagcttctcg acgggtccgg gatcgccctt cccgaacacc ggctcgaatg
    11461 accttccgcg ctcgccgagt gccgtctcca gaaggcgtgt ccggatcttc gcgttgacgc
    11521 ctcccgcgca atcgaggttg ttgagcacga tagcgccaac cccctcgtcg atcaggagga
    11581 gcaggtcggc cgagtagccg tacaccgcgc cgccgtgccc cacggtccga catccgtgca
    11641 acgtgcccac cgccatcccc agaccgaaac ccgcggaagg cgccggatcc ccccggacgg
    11701 gctggaccgt ggccatggcc cgaagcgtct cgggacgcag catccgatag tctcccgccg
    11761 tgccgcccgc caacaggcat cgcgcgaagc gggccagatc ctccgcggtc gaacagaggt
    11821 tgccggacgg cggcgtaccc aggtcaaagc gcggcgccgg gaacatcgag ccgtcgaagg
    11881 cggtcatcca cgcgtcactc gccctcgcgc ccggcggcgt gtggcggacg gtgaacccgg
    11941 agtgcctcat cccgagcggg accaggatcc cctcctcgat ctgccttccc cacgggattc
    12001 ccgtgacccg cgaaaggacg tacgccgcga tggtgggtcc gatgttggag tatttggctc
    12061 tcgttcccgg agccagaacg agatgggtgc cgaccatgct gagcaccgtc tcctcgatcg
    12121 ctggcccggt cgaatcgaag tagctcccga ccgggctctc ccgctggaga ccggcggtgt
    12181 gggagaggag gtgtcgcagg gtgactgcgg ccggcgcgcc ggatggatcc gcaaagacca
    12241 gctctggaca gcaagcatga atcggggcgt ccagatcgag cccgccggac tccacggcgc
    12301 gcatcacggc gaccgcggtg agcatcttgg agatcgaccc cgcccgatac actaccggaa
    12361 cggcagcggt ttccgcggat tcctcgcgtc cctgggagaa tcgacggtgc cagacggtgc
    12421 cttccgcgcc caccagcgcg acgtcgagca acggaatcga gtgccgccgc gtctcctccg
    12481 cgatgagtcg acagatcccc gccgtgtcca cggtacctcc caggaagccg taccacatcg
    12541 tcatgggatg tgcaaagggg cccgacgaac gctatgctcg cctggtgacg ccgtccggga
    12601 ccccgtccgc agtaccgtcg gcaaccccgt ccgtgggctc gtccgtgacc gttgaggagg
    12661 tgccgagcgc ctcgcgcgcg cacctcgtct ctctgctgga cctgagcttc gacggcatct
    12721 accgatggca cgcccgcagg acccttcccc aggttcgatg ggtgc
//
LOCUS       DASRZS010000674         3534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_753878, whole genome shotgun sequence.
ACCESSION   DASRZS010000674 DASRZS010000000
VERSION     DASRZS010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753878"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="VFH83_16455"
     CDS             complement(<1..1303)
                     /locus_tag="VFH83_16455"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HET6488021.1"
                     /translation="MKVVNQKSLDEKLGQATNRVSPLVVDQIKSFLLSAEDAQLYTIN
                     PYRFATRRKLDRSEALRAFLHLTKAGLFNLYWNIHCPACKGVTQHSNSLQSLKHADKC
                     GICTTEFNAGFDKSIEVSFAVNPSIIRPAEVDEFTKIVAGFELEPGINIELDRGESHY
                     LKSDLREGNYFVFVNGERKALNLVVVPGSSPETQKITWEYKSNLPFLTIAVANEGPAE
                     IVITNRERGRRAMTFARLAPQHWPNAAVVSSLQDFRDLFSSEMLSPNESFSIENLGFL
                     FTDLKGSTEMYERLGDSQAFAVVKEHFYIMERLVREHGGAIVKTIGDAVMAVFTDPGQ
                     ALRTAVEMIEAFDNMETATRLKNAVVVKIGVHHGPCIAVTLNERIDYFGTTVNIAARV
                     QGLSDGRDVMASGTIYDETNASDYVREKGWADEPFTTSLKGL"
     gene            complement(1335..2246)
                     /locus_tag="VFH83_16460"
     CDS             complement(1335..2246)
                     /locus_tag="VFH83_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET6488022.1"
                     /translation="MSIRPVRKIIQSTPTIEGAGVRLRRAFGFGDTDLYDPFLLLDDF
                     RNERPQDYLAGFPWHPHRGIETITYVLSGSVDHGDSLGNSGSIRTGDVQWMTAGSGII
                     HQEMPHGDDRGRVHGFQLWANLPSSLKMMKPRYQEVNAAEIPEATDDDGTRVRVICGS
                     MMGANGPVEGIAADPQYVDVWVPPDRRKTLPVDTVRHAFAYVFEGSGRFANASSPQPV
                     ATEYIGPEPDPGSLAEDRSLVLFDSGDEVTVQAGPKGIRFLLVSGHPLREPVAWRGPI
                     VMNTQEELRQAFDQLRDGSFLKVEPRR"
     gene            complement(2331..3080)
                     /locus_tag="VFH83_16465"
     CDS             complement(2331..3080)
                     /locus_tag="VFH83_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6488023.1"
                     /translation="MPLLAVDGLHIRYGALEALKGVDLVLEDREIVAVLGANGAGKST
                     LLRAISGLLPPAAGAIRLNGETLRGLPAYDIVLRGVSHAPEGRKVFATLTVQENLSLG
                     AFTRRHLRKEVAHARDRVFELFPILKDRREQLAGTLSGGEQQMLAIGRALMSAPRILL
                     LDEPSLGLAPILVNRIFRIIQEINHQGVAILLVEQNAHQALALASRGYVLETGRVSAH
                     GETAELQSDRRIQEAYLGGSAFSTRPRKPEP"
     gene            complement(3086..>3534)
                     /locus_tag="VFH83_16470"
     CDS             complement(3086..>3534)
                     /locus_tag="VFH83_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6488024.1"
                     /translation="LRSGLSAAIFRPPGQVAEELDALKVAEERLRQVGLWEARGELAR
                     NIPYGRQRMLEIARALATDPRLLVLDEPSSGLNPIETEELMRFIRGIIAAERLTVLLI
                     EHDMNVVMGISDWVTVMDGGRKIAEGTPQRIYHDPRVVEAYLGGGA"
BASE COUNT          605 a         1133 c         1136 g          660 t
ORIGIN      
        1 tcaagccctt caggctcgtg gtgaagggct catcggccca acccttctcc cgaacgtagt
       61 ccgaagcatt cgtctcgtcg tagatggttc cggacgccat gacgtcccgg ccgtcggaga
      121 gtccctgcac gcgggcggca atgttcaccg tggtgccgaa gtagtcgata cgctcgttca
      181 gggtaaccgc gatgcatggg ccgtgatgca ctccgatctt caccaccacc gcgttcttca
      241 gccgggtcgc cgtctccatg ttgtcgaacg cctcgatcat ctccacggcc gtgcgcagcg
      301 cctggccggg atccgtgaac accgccatca cggcgtctcc aatggtcttc acgatggctc
      361 cgccatgctc ccgcacgagg cgctccatga tgtagaagtg ttccttcacc acggcaaagg
      421 cctgagagtc tccgagtcgc tcgtacatct ccgtggaacc cttgaggtcc gtgaagagga
      481 acccgaggtt ctctatcgag aaggactcgt tcggggaaag catctccgag gagaacaggt
      541 ctcggaagtc ctgaaggctc gagaccaccg ccgcgttggg ccagtgctgg ggggccaggc
      601 gcgcgaaggt catggccctt cgaccccgct cgcggttggt gatcacgatc tccgccggtc
      661 cttcgttggc aacggcaatc gtcaggaagg gcaggttgct cttgtactcc caggtgatct
      721 tctgagtctc cggggaggag cccgggacca ccacgaggtt gagggccttt ctctcgccat
      781 tgacgaagac gaagtagttg ccctcccgca ggtccgactt cagatagtga ctctcacccc
      841 ggtccaattc gatgttgatc cccggctcga gctcgaatcc ggccacgatt ttggtgaact
      901 cgtcgacctc ggcgggccgg atgatcgatg ggttcacggc aaagctcacc tcgatgctct
      961 tgtcaaagcc ggcgttgaac tccgtggtgc agatcccgca cttgtcagcg tgcttgagcg
     1021 actgtagcga gttggaatgc tgggtgactc ccttgcacgc cggacagtgg atgttccagt
     1081 agagattgaa gagtccggcc ttggtaagat ggaggaaggc ccgcagcgct tccgatcgat
     1141 ccagcttgcg ccgcgtggcg aagcgatacg ggttgatggt gtagagctgt gcgtcctccg
     1201 cggacaagag gaagctcttg atctgatcca cgacgagcgg cgacacccgg ttggtggcct
     1261 gcccgagctt ctcgtccagg gacttctgat tgacgacttt caacggttcc tcctcgctta
     1321 gggggtgccc cggctcagcg ccggggctca accttcagga atgagccgtc gcgaagctga
     1381 tcgaacgcct ggcgaagctc ttcctgggta ttcatgacga tgggcccgcg ccaggccacc
     1441 ggctcacgca gtggatggcc ggagaccagc aggaagcgga tccccttggg cccggcctgg
     1501 acagtcacct cgtccccgga atcgaagagc accagcgacc gatcctccgc gagggagccg
     1561 gggtccggtt ccgggccgat gtattctgtg gcaaccggtt gcgggctgga tgcgttggcg
     1621 aaccgcccgg atccctcgaa gacgtacgca aaggcgtggc gcaccgtgtc cacgggcaga
     1681 gtcttgcgcc gatcgggagg gacccagaca tcgacgtact gcgggtccgc cgcaatccct
     1741 tccaccggac cgttggcgcc catcatggag ccgcagatca cgcgcacccg ggtaccgtcg
     1801 tcgtcggtcg cttccggaat ctccgccgcg ttgacctcct ggtaccgtgg cttcatcatc
     1861 ttcagggagg aagggagatt ggcccagagc tggaacccgt ggacccggcc gcggtcgtcc
     1921 ccgtgaggca tctcctggtg aatgatcccg cttccggcgg tcatccactg tacgtctcct
     1981 gtgcggatgc tgccgctgtt tcccaggcta tccccgtggt ccacgctgcc cgagagcacg
     2041 taggtgatcg tctcgatccc tcggtgtggg tgccagggga acccagccag atagtcctgc
     2101 ggacgctcgt ttcggaagtc gtcgaggagc aggaacggat cgtagaggtc cgtgtcgccg
     2161 aacccgaagg ctcgacgcaa ccttaccccg gcaccctcta tggttggcgt ggactggatg
     2221 atcttgcgga ccggacgaat ggacacacgg acctcccggg aagcttcgtc ccgagaagat
     2281 acatactttg tcgcggaacg gcgatttcgg gagacgtgag cgtcgcgggt tcaaggctca
     2341 ggtttccgcg gccgggtgct gaacgcggaa ccgcccaggt aggcctcctg gattctccgg
     2401 tcagactgga gctccgcggt ttcgccatgg gccgagacgc gcccggtctc caggacgtac
     2461 ccgcggcttg ccagggcgag ggcctggtgc gcgttctgct ccaccaggag gatagccacg
     2521 ccctggtggt tgatctcctg gatgatccgg aagatccggt tgaccaggat cggggcgagc
     2581 ccgagggatg gctcgtccag gaggaggatc cttggggcgc tcatcaaggc gcggcctatg
     2641 gcgagcatct gctgctcgcc gccggacagc gtgccagcca gctgctcccg tcggtctttg
     2701 aggatcggga agagctcgaa cacgcgatcc cgggcgtgag ccacttcttt gcgaaggtgg
     2761 cggcgggtaa acgcgccgag actgaggttc tcctggacgg tcaacgtcgc aaagaccttg
     2821 cggccttccg gggcgtggga aacacctcgg aggacgatgt cgtatgccgg caagccgcgg
     2881 agcgtttcgc cattcagccg gatggctccc gccgccggcg gcaacaggcc cgagatggca
     2941 cgaagcagcg tgctcttgcc ggcgccgttg gcgccgagca ccgcgacgat ctcccggtcc
     3001 tccagaacaa ggtccacgcc cttgagcgcc tccagggcgc cgtagcggat gtgaaggcca
     3061 tcaacggcaa gaagaggcag gccgctcacg cacctcctcc cagataggct tccaccaccc
     3121 ttgggtcgtg gtatatccgc tgtggcgtac cctcggcgat ctttctgccc ccgtccatga
     3181 cggtgaccca gtccgagatc cccatcacca cgttcatgtc gtgctcgatc aggagcacgg
     3241 tgaggcgctc ggcggcgatg atcccccgga tgaatcgcat gagctcttcc gtctcgatgg
     3301 gattcagccc gctggacggc tcgtccagga ccaggagccg tgggtccgtg gccagggcgc
     3361 gggcgatctc cagcatccgc tgtcggccgt acgggatgtt gcgagccagc tcgccgcggg
     3421 cctcccagag ccccacctgt ctcaaccgtt cctccgccac cttcagcgcg tccagctcct
     3481 ccgcgacctg gccgggggga cgaaagattg ccgcggacaa acccgagcgc aggc
//
LOCUS       DASRZS010000675         2618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3441510, whole genome shotgun sequence.
ACCESSION   DASRZS010000675 DASRZS010000000
VERSION     DASRZS010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3441510"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VFH83_16475"
     CDS             complement(<1..862)
                     /locus_tag="VFH83_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6488025.1"
                     /translation="MSLTPGLDSDRTAPLLQVEELKTYFHTFEGVLHAVDGVTFSVYS
                     GRNLGLIGESGCGKSVTALSVMQIQSKPGRIDGGSVRWRTRTLAGPADGGSGGTEESV
                     IDLAALKPDSPALRMIRGNEIAMVFQEPMTCFSPYYTIGNQIREAILIHTRRSRDPQT
                     RTQAEARSIQMLARVAIPNPKKTYAMYPHELSGGMRQRAMIAMALVGQPRLLIADEPT
                     TALDVTTQAQILALLRRLQREKQIALMLITHDMGVIAQMADDVAVMYLGRMVEFAPVR
                     ELFAAPKHPYT"
     gene            complement(859..1962)
                     /locus_tag="VFH83_16480"
     CDS             complement(859..1962)
                     /locus_tag="VFH83_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6488026.1"
                     /translation="MSKGRQSEQIYRASQWQLMAWKLRRHKLAVAGGIILVVFYLAAV
                     FCEFLSPYDPSQKDTQRINAPPMRVHWVDANGRFRLRPFYYDYTVSRDPDTLEWLYKE
                     DQAQPRDLRLFVNGDRYRMWSLFVGTIHLFGSSSGRVYLLGTDQLGRDMLSRDLYGSR
                     ISLSIGLVGIAVSMVLGIIIGGVSGLLGGVVDLVIQRIIEFLRSIPTLPLWMGLSAAI
                     PLKWPPLRIYAIVVVVLSLVGWTDLGRVVRGKFMSLVKEDFVMAARLLNARSSWVISR
                     HLVPAFASYLIVNLTLSIPGMILGETALSFLGLGLQPPVISWGTLLQSAQNVRTIALY
                     PWQAIPVLLVVVTVLAFNFLGDGLRDAADPYTV"
     gene            complement(1985..>2618)
                     /locus_tag="VFH83_16485"
     CDS             complement(1985..>2618)
                     /locus_tag="VFH83_16485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6488027.1"
                     /translation="LVSFPIAFYASRHQYSVGDYLASTVGFLGLAIPNFLLALVLMYL
                     AYVLFGADVTGLVSTKYQTSPWNLPKILDMVKRLWLPLVVIGMAGTAGLIRILRGCLL
                     DELNRPYVITARAKGMQERRLLWKYPVRAAVNPLISTIGWALPAIISGEAIVSIVLGY
                     QTIGPLFLNALMNKDMFLGGAILLILSFLTIVGTLISDILLVVLDPRLRS"
BASE COUNT          503 a          845 c          853 g          417 t
ORIGIN      
        1 gcgtgtacgg gtgcttcggc gcggcgaaca actcacgcac cggcgcgaac tccaccatgc
       61 gtccgagata catcaccgcc acgtcgtccg ccatctgtgc gatcacgccc atgtcgtggg
      121 tgatcagcat cagcgcgatc tgcttctcgc gttgcagccg gcgcagcagt gccaggattt
      181 gcgcctgggt cgtgacgtcc agagcggtgg tcggctcatc ggcgatcagg agccggggct
      241 ggccgaccag cgccatggcg atcatggccc tctgccgcat cccgccggag agctcgtggg
      301 gatacatcgc ataggtcttc ttggggttcg ggattgccac gcgggccagc atctggatgg
      361 agcgcgcctc ggcctgggtc cgtgtttgcg ggtcacggga ccggcgcgtg tggatcagga
      421 tggcctcccg gatctggttc ccgatggtgt agtacgggct gaaacaggtc ataggctcct
      481 ggaagaccat ggcaatctcg ttgccacgga tcatgcgcag ggccggggag tccggcttga
      541 gggcagccag gtcgatcacg gattcctcgg tgccgccgct acccccatcg gcgggaccgg
      601 cgagggttcg tgtcctccac cgaacggacc cgccgtcgat ccgacccggc ttgctctgga
      661 tctgcatcac ggacagcgcc gtcacgctct tgccgcagcc gctctctccg atgagcccca
      721 ggttgcgtcc ggagtacacg gagaaggtca cgccgtccac ggcatgcagg acgccctcga
      781 aggtatggaa ataggtcttc agctcctcaa cctggaggag cggcgccgtc ctgtctgagt
      841 ccagaccagg tgtcaggctc atacggtgta cgggtccgcg gcatcgcgaa gcccgtctcc
      901 cagaaagttg aacgccaaca ccgtcacgac caccaggaga accgggatgg cctgccacgg
      961 atagagggcg atggtccgca cgttctgcgc gctctgcagc agcgtccccc aactgatcac
     1021 cggaggctgg agtcccagcc ccaggaagct gagcgcggtc tcccccagga tcatgccggg
     1081 gatcgatagc gtcaggttca cgatgaggta gctcgcgaag gcgggcacca ggtgtcggga
     1141 aatgacccag ctcgagcgcg cgttcaggag ccgggccgcc atgacgaagt cctctttcac
     1201 cagggacatg aacttgcccc ggaccacacg tcccaggtcg gtccacccga ccagggaaag
     1261 caccaccacg acgatggcat agatgcgcag cggcggccac ttgagcggaa tggcggcgga
     1321 cagccccatc cacaacggga gggtcgggat ggacctcaag aactcgatga tcctctggat
     1381 caccaggtcc accacgcctc ccaggagacc cgagacgccg ccgatgatga tgcccaggac
     1441 catggagacc gcgatgccga ccaggccgat ggaaagggag atccgcgatc catagagatc
     1501 ccgagagagc atgtcccgcc cgagctggtc ggtgcccaac aggtagaccc gccccgagga
     1561 agagccaaag aggtggatcg tgccgacgaa gaggctccac atccggtagc ggtccccgtt
     1621 cacgaaaagt cgcagatccc gcggctgagc ctggtcctcc ttgtagagcc attccagcgt
     1681 gtcggggtcc cggctcaccg tgtagtcata atagaagggg cgcagccgaa acctgccgtt
     1741 cgcgtccacc cagtgcacgc gcatgggcgg cgcgttgatc cgttgggtgt ccttctgcga
     1801 cggatcgtac ggtgagagga actcgcagaa gacagccgcc aggtagaaga ccacgaggat
     1861 gatcccgccc gcgacggcca gcttgtgccg tcggagcttc cacgccatga gctgccactg
     1921 ggacgcccgg tagatctgct cgctctgccg ccccttgctc acgtgctccc tcctccgtcc
     1981 ccggtcagct tcgaagccgc gggtccagca ccaccagaag gatgtcggag atcagagttc
     2041 ctacgatcgt gaggaagctc aggatcagga ggatggcccc cccaaggaac atgtccttgt
     2101 tcatcagggc gttgaggaac aggggtccta tggtctggta gccaagcacg atggagacga
     2161 tggcctcccc ggagatgatg gccgggagcg cccacccgat ggtgctgatg agagggttca
     2221 ccgcggcgcg caccggatac ttccacagga ggcggcgctc ctgcattccc ttggcgcggg
     2281 ccgtgatcac gtacggccgg ttcagctcgt ccagcagaca cccccgcagg atccggatga
     2341 gacccgcggt ccccgccatc ccgatgacca ccaggggcag ccagagccgc ttcaccatgt
     2401 ccaggatctt gggcaggttc cacggcgagg tctggtactt cgtggagacg agccccgtca
     2461 cgtcggcgcc gaaaagcacg taggccaggt acatcagcac cagcgccagg aggaagttcg
     2521 ggatggccag acccaggaag cccaccgtgc tggccaggta gtcgcccacg gaatactggt
     2581 ggcgggacgc atagaaggcg atggggaacg agaccagc
//
LOCUS       DASRZS010000676         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5384824, whole genome shotgun sequence.
ACCESSION   DASRZS010000676 DASRZS010000000
VERSION     DASRZS010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5384824"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /gene="secD"
                     /locus_tag="VFH83_16490"
     CDS             <1..686
                     /gene="secD"
                     /locus_tag="VFH83_16490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET6488028.1"
                     /translation="EQATINDAIRSQVQVSGGFNAQEAADLALVLRTGSLPVPLDITS
                     QQTIGASLGQDAINQGIKAAVIGIGMVMLFMLGYYRRAGFNAVLAQLLHLYINVAILS
                     VFNFTLTLTAIAGLVLTIGMAVDANVLIFERIKEESRLGKSVQAVISHGFSRAFSAIL
                     DSNVTTIIAALVLTQLAKGSIQGFAVTLLVGNLATLFAAVFVSRLIFDFSIDVLHTKH
                     ILVTWRRTA"
     gene            683..2287
                     /gene="secF"
                     /locus_tag="VFH83_16495"
     CDS             683..2287
                     /gene="secF"
                     /locus_tag="VFH83_16495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET6488029.1"
                     /translation="MKRVIRFSRARYYFFGFSGTLILIGILGLILNHGFNLGVDFRAG
                     LSIQFHIAPRSLAIQYTGPDSALLDVPAGEQALTAAGNIIITTTAASNGAKTSYPFKY
                     SDYASISALADGIRKVPGVTVDIKGDATLPPSDLLPLVRPADITGKAAYLNLNPAVVP
                     NQPVTISDVRSTLDPMGEHDLQAVGAPANQEFISRVATTTEDQSFQTTTQNKLTDLLS
                     AKYGAEQVIIESTEFVGPRMAQALTTQTMWLVLIAIVLILVYMMFRFHPAIYAVAAVL
                     GILHDAVIMLGFDAVFRVQVDAGTIAAILTILGYSINDTIVNFDRARENLGLMRGESL
                     RTILDTSVTQTLSRTFITSGATMLTVVALFILTSGSIKNFAFNMIVGIAEGTYSTFIS
                     AFIVIEWTTARDRRRKSAELGRYGISEKSGAAEGKLAMAGAVAASGAPMVTAQVAAAE
                     EAEESVEDEAEGADGAVREPAADGRPTDADTPTGDGDGAQATTPAGGQSGQPTAGQTV
                     ARPANVMAFPGNTGSRKHKKHRRRHH"
     gene            2291..2947
                     /locus_tag="VFH83_16500"
     CDS             2291..2947
                     /locus_tag="VFH83_16500"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6488030.1"
                     /translation="MKLLCLSDIHGEVAGLQGFLKEVDGVDAVVVAGDITHLGGAPEA
                     HAILDPLLETGVRVLAVAGNMDREGVRDLLREMDLDLHGRGLVLGSVGIMGLGGGTQS
                     PFGTPWELTESDARNALASGVIQVSSASYKVLVSHAPPQGTRIDRSSTGRHVGSKPVR
                     EFLLSGAVDLCISGHIHEAAGEDTVGKARCLNLGAFKEGRFALVTIERSQASVVWRKP
                     "
     gene            2944..>3282
                     /locus_tag="VFH83_16505"
     CDS             2944..>3282
                     /locus_tag="VFH83_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET6488031.1"
                     /translation="MNPTALSAIANSVRSLTIDAVEKATSGHPGLPMGCAELGALLYG
                     DVLNHLPQDPRWINRDRFVLSAGHGCMLLYSLLHLSGYDLSVEDIKNFRQLGSRTPGH
                     PEWGLTPGVEA"
BASE COUNT          571 a         1144 c         1013 g          554 t
ORIGIN      
        1 aggaacaggc caccatcaac gacgccatcc gaagccaggt ccaggtatcc ggcgggttca
       61 acgcccagga agcggcggac ctggccctgg tgctgcgcac cggctccctg cccgtgccgc
      121 tggatatcac cagccagcag accatcggtg cctccctggg tcaggacgcc atcaaccagg
      181 gaatcaaggc cgccgtcatc ggtatcggca tggtcatgct cttcatgctc gggtactacc
      241 gacgcgcggg gttcaacgcc gtcctggccc agctgctcca cctctacatc aacgtggcca
      301 ttctgtcggt gttcaacttc accctgacgc tcaccgccat cgccggcctg gtcctgacca
      361 tcggaatggc cgtggacgcc aatgtgctga tcttcgagcg gatcaaggag gagtcccgtc
      421 tgggcaagtc cgtccaggcg gtcatcagcc acgggttctc ccgggcgttc agcgccatcc
      481 tggactccaa cgtgaccacg atcatcgccg cgctggtgct gacccagctc gccaagggca
      541 gcatccaggg cttcgccgtc accctgctgg tgggcaacct ggcaacgctg ttcgcggccg
      601 tattcgtgtc ccggctgatc ttcgacttca gcattgacgt gctgcacacc aagcacatcc
      661 tcgtgacctg gagaaggacc gcatgaagag agtcatccgt ttctcgagag ctcggtacta
      721 cttcttcggg ttctccggca cgctgatcct gatcgggatc ctcggtctga tcctcaacca
      781 cggcttcaac ctgggggtcg atttccgagc gggtctttcg atccagttcc acattgcgcc
      841 ccgctcgctg gccatccagt acacgggccc cgattccgcc ctgctcgacg tccccgcggg
      901 agagcaggcc ctgaccgcgg ccggcaacat catcatcacc accaccgcgg cgtccaacgg
      961 cgccaagacg agctacccct tcaaatactc ggactacgcc tccatctccg cgctggcgga
     1021 cggcattcgc aaggtccccg gcgtgaccgt ggacatcaag ggggacgcaa cactgccgcc
     1081 atcggacctg ctgcccctgg tgcgtcccgc ggacatcacg ggcaaggcgg cctacctgaa
     1141 cctcaatccg gccgtcgtgc ccaaccaacc cgtcacgatc tcggacgtcc gctccaccct
     1201 ggatcccatg ggagagcacg acctgcaggc ggtgggcgct cccgcaaacc aggagttcat
     1261 cagccgcgtg gccacgacca cggaggacca gtccttccag accaccaccc agaacaagct
     1321 cacggacctt ctctcggcga agtacggcgc ggagcaggtc atcatcgaga gcaccgagtt
     1381 cgtgggaccc cggatggccc aggccctgac cacgcagacc atgtggttgg tgctgatcgc
     1441 catcgtgctg atcctggtct acatgatgtt ccggttccac ccggccatct acgccgtcgc
     1501 cgccgtcctg gggatcctgc atgacgccgt gatcatgctg ggcttcgacg cggtctttcg
     1561 ggtccaggtg gacgcgggca ccatagccgc catcctcacc atcctgggct actcgatcaa
     1621 cgacacgatc gtcaacttcg accgcgctcg agagaacctg gggctgatgc ggggcgagtc
     1681 ccttcggacc atcctggata ccagcgtcac ccagacgctg agccggacgt tcatcacctc
     1741 cggcgccacg atgctcaccg tggtggcgct gttcatcctg accagcggct ccatcaagaa
     1801 cttcgccttc aacatgatcg tcggtatcgc ggaaggcacg tactccacct tcatctccgc
     1861 gttcatcgtc atcgagtgga ccaccgcccg ggatcgccgg cgcaagagcg cggagctggg
     1921 tcggtatggc atcagcgaga agagcggcgc ggcggaaggc aagctcgcca tggccggagc
     1981 cgttgccgcc tccggcgcgc cgatggtcac cgcccaggtg gcggccgccg aggaggccga
     2041 ggagtcggtg gaggacgagg ccgaaggtgc cgacggcgcg gtccgggaac cggcggcaga
     2101 cgggcggccc acggatgcgg acaccccgac gggggacggt gacggagcgc aggccacgac
     2161 gccggcgggt gggcagtccg ggcagccgac cgccggccag accgtggcgc ggccggccaa
     2221 cgtgatggcg ttccccggca atacagggtc cagaaagcac aagaagcacc gtagacggca
     2281 ccactaagcc gtgaagctcc tctgtctgtc ggacatccat ggggaagtcg ccgggctcca
     2341 gggcttcctc aaggaggtcg atggcgtcga cgccgtcgtc gtagccgggg acatcaccca
     2401 cctgggcggc gccccggagg cgcacgcaat cctcgatccc ctgctggaaa ccggcgtgcg
     2461 agtcctcgcc gtggccggca acatggaccg tgagggggtc cgagacctgc tccgcgagat
     2521 ggacctggac ctccacggcc gtggcctggt gctggggtcc gtcggcatca tgggtctggg
     2581 cggcgggacc cagtcaccgt tcggcacgcc gtgggagctc accgagtccg atgcccgaaa
     2641 cgccctagcc tcgggcgtca tccaggtttc cagcgcatca tacaaggtgc tcgtgtccca
     2701 cgcgcctccc cagggtaccc gcatcgatcg aagctccacg ggccgccacg tcggctcgaa
     2761 gcccgtccgg gagttcctgt tgtccggtgc cgtggatctc tgcatttctg gccacatcca
     2821 tgaggccgcc ggggaggata ccgttggcaa ggcccggtgc ctgaacctgg gggccttcaa
     2881 agaggggcga ttcgccctgg tgaccatcga gcgatctcag gccagcgttg tctggaggaa
     2941 accatgaacc caaccgctct gtccgctatc gccaactccg tgcgatcgct taccatcgac
     3001 gccgttgaga aggccacgtc cggccacccc gggcttccca tggggtgcgc cgagctgggc
     3061 gctcttctct atggggacgt gctcaatcac ctgccgcaag atccgcgttg gatcaaccgg
     3121 gaccggttcg tcctgtcggc gggacacgga tgcatgctcc tgtactccct gcttcacctc
     3181 tccggctatg acctgagcgt tgaggatatc aagaacttcc ggcagctagg ctccaggacc
     3241 cccggccatc cggagtgggg cctgaccccc ggagtcgagg ct
//
LOCUS       DASRZS010000677         3232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4598128, whole genome shotgun sequence.
ACCESSION   DASRZS010000677 DASRZS010000000
VERSION     DASRZS010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4598128"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFH83_16510"
     CDS             complement(<1..235)
                     /locus_tag="VFH83_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HET6488032.1"
                     /translation="MPATAELLVNPRRVLGRRDGKIYGQFLEHFHRQVYGGVFEPGSP
                     LADSRGFRSDVVKALRAIRTPVLRWPGGCFVSAY"
     gene            complement(242..1750)
                     /locus_tag="VFH83_16515"
     CDS             complement(242..1750)
                     /locus_tag="VFH83_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008338403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HET6488033.1"
                     /translation="MLSARIMIDRDFVLSTVDDRLFGSFIEHLGRAVYGGIYERGHPA
                     SDGDGFRADVLELVRELAVPIVRYPGGNYVSAFNWEDGVGPTGDRPRRLDLAWRSVEP
                     NTVGLNEFARWAKMAGSEVMVALNLGTRGIDAARNLVEYCNHPGGSRWSDLRRSHGTP
                     EPHGFKVWCLGNEMDGPWQVGYKTADEYGRLASEVGKALKLFDPTLQLVACGSSNSEM
                     PSFPQWEATVLDHCYETVDFISLHSYFKNFEDDLPTFLGESVEMDRFIRTVIGTVDYV
                     KARKRSRRTVNLSFDEWNVWFHSRQADEKVPPWTIGPPLLEDVYTMEDALVVGCCLIT
                     LLRHAPRVRMACLAQLVNVIAPIMTETGGPAWRQTIFYPFLHASRYGRGEVLETMVRS
                     PVYQNRKHGEVPYLETIATINREAGELSIFAVNRNLQEDLLLEGAVAGFRGLEVMEHI
                     VLAHRDNKASNTRAKPDTVVPARRNATRVEGSTLQSRLPPLSWNVIRLKTGA"
     gene            complement(1760..2611)
                     /locus_tag="VFH83_16520"
     CDS             complement(1760..2611)
                     /locus_tag="VFH83_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6488034.1"
                     /translation="MRTLRAADTRTHILVSLFFVLVCLAFLFPIYSLLLASFRPGRDL
                     MRFGISVKTLTPSGLTLKYLASLLTARGGAYTVWFRNSVILVILQTGLSLFFSSLVGY
                     GLGVYEFKGRRLLFSLVLLVLVVPIQILILPLYNLLIFFKSMNSFRGIVAPFIVSPFA
                     IFFFRQYAIGIPMDFIDAGRIDGLREGGIFFRIISPLMKPAFGAMAILVALQSWNNFL
                     WPLIVLRTGNMFTLPIGLASLLSPYGNNYDVLISGSLMATLPIIIVFFIFQRYFIAGL
                     TAGGIKG"
     gene            complement(2608..>3232)
                     /locus_tag="VFH83_16525"
     CDS             complement(2608..>3232)
                     /locus_tag="VFH83_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6488035.1"
                     /translation="NRPGNPASVVFRGALFLPTLVSVVVAGTIIRLLFAGSPSAIVNT
                     LLRPLGVKPTDWLLGGSASAMTLLVGMATWRWTGVNIVYFMSGLQNIPRDLYEAADID
                     GASAWYKLFRVTVPLLKPTIVFVTTISVFGGFAMFEESYILWAGQSGGGSPNNVGLTM
                     VGYIYLKGFQGGALGLGSAIGMILLVIVLAVSFLVLNLFGFFKREEQ"
BASE COUNT          614 a         1030 c         1083 g          505 t
ORIGIN      
        1 ggtacgccga gacaaagcag ccgcccggcc agcgcaacac cggggtgcgg atagctctca
       61 gcgccttcac cacgtccgag cggaagccgc gtgagtcggc aagcggcgag ccgggctcga
      121 acacgccgcc gtagacctgg cggtggaagt gctccaggaa ctggccgtag attttcccgt
      181 cccggcggcc gaggacacgt cgcgggttga ccaggagctc tgccgtggcg ggcatgacgg
      241 ctcaggctcc ggtcttcagc cggatcacgt tccacgacaa cggcggaagc cgggactgga
      301 gagtgctccc ctcgacgcgc gtggcgttcc tgcgcgcggg gaccaccgtg tcgggcttcg
      361 ccctcgtgtt ggacgccttg ttgtcccggt gggcgagaac gatgtgctcc atcacctcca
      421 gaccgcggaa gcccgccacg gcgccctcca gcagcaggtc ttcctgcagg ttgcggttca
      481 ccgcaaagat cgagagctct ccggcctccc ggttgatcgt ggcgatggtc tcgaggtacg
      541 gcacctcgcc gtgcttcctg ttctggtaca ccggggatcg caccatggtc tccaggactt
      601 cgccccggcc gtaccgggag gcatggagga agggatagaa gatggtctgc cgccacgccg
      661 ggccgccggt ctcggtcatg atgggggcaa tcacgttcac cagctgcgcg aggcaggcca
      721 tgcgcacgcg cggcgcgtgg cgcagcagcg tgatcaggca gcagcccacc acgagggcat
      781 cctccatcgt gtagacgtcc tccaggaggg gcggcccgat ggtccacgga gggaccttct
      841 cgtccgcctg gcgggagtgg aaccacacgt tccactcgtc gaaggagagg ttcaccgtcc
      901 gacggctgcg cttccgtgcc ttcacgtaat ccaccgtgcc gatgaccgtg cggatgaacc
      961 ggtccatctc caccgactcg ccgaggaatg tcggcaggtc gtcctcgaag ttcttgaagt
     1021 agctgtgcag cgagatgaaa tccaccgttt cgtagcagtg gtccaggacg gtcgcctccc
     1081 actggggaaa cgacggcatc tccgagttgg agcttccgca cgccaccagt tgcagcgtgg
     1141 gatcgaacag cttcagtgct ttgcccacct ccgaggccag tcgcccgtac tcgtccgcgg
     1201 tcttgtaccc cacctgccac ggaccgtcca tctcgtttcc caggcaccat accttgaagc
     1261 cgtgcggttc gggggtgcca tgggaccggc gcaggtcgct ccaccgcgac ccgccgggat
     1321 ggttacaata ctccaccagg ttgcgcgccg cgtcgatccc gcgtgtcccg aggttgagcg
     1381 ccaccatgac ttccgatccc gccatttttg cccagcgcgc aaactcgttc aggccgaccg
     1441 tgttcggctc cacggagcgc cacgcgaggt cgaggcggcg gggccggtcg cccgtcggac
     1501 ccacgccgtc ctcccagttg aacgcggaca cgtagttgcc cccggggtag cggacgatcg
     1561 gcaccgccag ctcccgcacc agctcgagca cgtccgcgcg gaagccgtcg ccgtcgctcg
     1621 ccgggtgtcc ccgctcgtag atgccgccgt agacggcccg gccgaggtgc tcgatgaagg
     1681 agccgaacaa ccggtcatcg acggtggaaa ggacgaaatc gcgatcgatc atgatgcggg
     1741 cgctcagcat ggaggactcc taccccttga ttccaccggc ggtcaggccg gcgatgaagt
     1801 atctctggaa aatgaaaaag acgatgatga tcgggagggt cgccatgagc gacccggaga
     1861 tcagcacgtc gtagttgttg ccgtagggtg aaagaaggct ggccagcccg atgggcaggg
     1921 tgaacatgtt ccccgtgcgc agcacgatca gcggccagag gaaattgttc cagctctgca
     1981 gggccaccag gatcgccatg gcgccgaacg cgggcttcat gagcggcgag atgatgcgga
     2041 aaaagatgcc gccctcccgg aggccgtcga tcctgcccgc gtcgatgaag tccatcggga
     2101 tcccgatcgc gtactggcgg aagaagaaga tcgcgaaggg agagacgatg aacggcgcca
     2161 cgatcccgcg gaagctgttc atggacttga agaagatcag caggttgtag agcggcagga
     2221 tgaggatctg gatgggcacc accagcacca ggaggaccag gctgaagagg agcctccgcc
     2281 ccttgaactc gtacaccccc agcccgtagc cgaccaggga cgagaagaac aggctgagcc
     2341 cggtctgcag gatgacgagg atcacgctgt tgcggaacca cacggtgtag gcgccgcccc
     2401 gtgccgtcag gaggctcgcg aggtacttca gggtcaggcc gctgggagtc agcgtcttca
     2461 cggagatccc gaaccgcatg aggtctctcc ccggtcggaa cgaggccagg agcagcgagt
     2521 agatcgggaa gaggaaggcc aggcagacca ggacgaagaa gagggagacg aggatgtgtg
     2581 tccgcgtgtc ggcggcccgc agcgttctca ttgctcttcc ctcttgaaaa agccgaacag
     2641 gttcagcacc aggaagctga ccgccaggac gatcaccagg aggatcatgc cgatggcgga
     2701 ccccagcccc agcgcgcctc cctggaagcc cttgaggtag atgtagccga ccatggtgag
     2761 gccgacgttg ttcggggagc ctccaccgga ctggcccgcc cagaggatgt agctctcctc
     2821 gaacatggcg aagccgccga acacgctgat ggtggtgacg aagacgattg tcggcttcag
     2881 cagcgggacg gtgacacgga agagcttgta ccaggcggag gcgccgtcga tgtccgccgc
     2941 ctcgtacagg tcccggggga tgttctgcag gcccgacatg aagtagacga tgttgacccc
     3001 cgtccatcgc caggtggcca ttcccaccag gagtgtcatt gccgaagcgg agccacccag
     3061 gagccagtcc gtgggcttca cgccgagggg ccggagcagg gtgttcacga tggccgaggg
     3121 gctcccggca aagagcagcc ggatgatggt gcccgccacc accacggaca cgagggtggg
     3181 gagaaacagc gcgccgcgga acaccacgct ggccgggttg ccgggcctgt tc
//
LOCUS       DASRZS010000678         3815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4598350, whole genome shotgun sequence.
ACCESSION   DASRZS010000678 DASRZS010000000
VERSION     DASRZS010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4598350"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFH83_16530"
     CDS             <1..798
                     /locus_tag="VFH83_16530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6488036.1"
                     /translation="IFQSIRLALLDYNVINPHLSRYIGWVNFRKLFHDPYVWQALGNT
                     AYFSFGTVVPGIILSLILSLIITERWFRFQHFARVIVFIPFVISMTIAGLLWSWLYAP
                     TVGFFNFVLTSVGLPALTYLGNPRLAMLCIIIMAVWKGLGYNVTIWSAGILGLPKDYH
                     DAAVIDGATFSQELFRIRLPLLRPVLMFLTVLGFISSFQSFDAVYVLTQGGPLNRTRV
                     LVFYLWQNAFQQIKMGYASTIAWLLFVILIVLTFLQFRGYGRQEVLF"
     gene            795..1682
                     /locus_tag="VFH83_16535"
     CDS             795..1682
                     /locus_tag="VFH83_16535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6488037.1"
                     /translation="MSRRIRARGGRGGRGARGVRRAWRSTYRVLFHVLVALVMFTSLF
                     PFLWMLSTSFKTLNEALTWPPTVFPKAPNLLAYQDILTKTGIFHYLANTVIYAGAGAS
                     LAILFSSLAGFAFAKYRFAGRRVLFALVLATMMIPFQITLIPVFLILKTLGWINTFMG
                     LIVPGLGDAFGIFLIRQFAFGVPDELLDSARIDGAGEVRIFSTIFLPLCAPAVGTLLV
                     LSFMNRWNDLFWPLIVTTGDKLRTIQLGLIAMQMSLWETIWPQLTASMVLVLIPILLL
                     YIFLQRYFTQGIVLTGLKG"
     gene            1697..3769
                     /locus_tag="VFH83_16540"
     CDS             1697..3769
                     /locus_tag="VFH83_16540"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HET6488038.1"
                     /translation="MPELENEDIRVTLDDAGSLVGLYDKRLKRSYLGASQFSGLFRLM
                     VPSPQWDGRHFDSRTSKGIRVRAVSATEAEVTCGELTSGTDRCAAAVTARLRLDGDCL
                     IASLEIENQGDGLINEALFPFIGGFATDTGDLTLFIPTQHARRMIRPFQDLGDGNHKG
                     WTRENNRHFIRYPTHLATAWVDYSSFRGGVGIEARSPQFEVTDFCFERAIRKSPRAAE
                     ENAQGLYAVLQFHPNLARGGRWRSPDVVVRLHRGDWHVTADAHREWLETVVQPTVTPE
                     AFRNGLGWHFYFMKVQDGTFFRTYDDLPAMAKASQAAGIDYIMVFGWQQKGHDNYYSF
                     GYYPNEDWGGVARLKEKIREVRAQGCHVIPFFNGTLLDITTPEYKEYGHRWPVIGRLG
                     SPYWGGDFSRGNFDFCFQNANNNTTERNMTLLDLCVTGREAREWEKDTIRRISQEYGF
                     GNIQLDQIAHKCYVCYDPAHGHSAPQYASTRELTALLAEVRSALREHDPDGVMVGEGF
                     TDMTAQYCDGFWSWSQLRFPEIIRYSVPWMAYSHETDANEYAEVNTCFINRILLDLKI
                     EGGDGTLADFPQFTAHLRRLSDLKKRLGPTYIDGLYRDEEGITVEGDTVRAKVYHDRA
                     RKTACVVAANLTNRRASATLRLSVPVPGDMRVVSWAGGEERTGARSSFRLQLDPYEVR
                     GFEFSMQR"
BASE COUNT          633 a         1272 c         1188 g          722 t
ORIGIN      
        1 atcttccagt ccatccgcct ggcgctgctg gactacaacg tcatcaaccc acacctgtcc
       61 cggtacatcg gctgggtgaa cttccgaaag ctcttccacg atccatacgt ctggcaggca
      121 ctggggaata cggcgtactt cagcttcggg accgtggtgc cgggcatcat cctgtcgctg
      181 atcctttccc tcatcatcac ggagcgctgg ttccgcttcc agcatttcgc ccgggtgatc
      241 gtcttcatcc cgttcgtcat ctccatgacc atcgcggggc tgctgtggtc ctggttgtac
      301 gcccccaccg tcggcttctt caacttcgtg ctgacctccg tggggcttcc cgcgctcacc
      361 tacctgggca atccccggct ggccatgctg tgcatcatca tcatggccgt ctggaagggg
      421 ctgggctaca acgtgaccat ctggagcgcc ggcatcctgg gcctccccaa ggactaccac
      481 gatgcggcgg tcatcgacgg agccaccttc tcccaggagc tcttccggat ccggcttccc
      541 ctgctgcggc ccgtgctcat gttcctgacc gtgctgggct tcatctcatc cttccagagc
      601 tttgacgcgg tctacgtcct gacccagggc ggaccgctga accggacccg ggtgctcgtc
      661 ttctacctct ggcagaacgc cttccagcag atcaagatgg ggtacgcgtc caccatcgcg
      721 tggctcctgt tcgtcatcct gatcgtgctg accttcctgc agttccgcgg ctatggccgg
      781 caggaggtgc tgttttgagt cgccggattc gagctcgggg cggacgaggc ggaaggggcg
      841 cacggggcgt ccgacgagcc tggcgctcca cctaccgcgt cctcttccac gtgctcgtgg
      901 cgctggtcat gttcacgtct ctgtttccgt tcttgtggat gctctccacg tccttcaaga
      961 ccctgaacga ggcgctgacc tggcccccca ccgtgttccc caaggcgccc aacctcctgg
     1021 cctaccagga catcctgacc aagaccggga tcttccacta cctggccaac acggtgatct
     1081 atgccggggc aggagcctcc ctggccatcc tgttctcctc cctggccggg ttcgccttcg
     1141 ccaaataccg attcgcgggt cggcgggtcc tcttcgcgct cgtcctggcg accatgatga
     1201 tcccgttcca gatcacgctc atcccggtct tcctgatcct gaagacgctt ggctggatca
     1261 acacgttcat gggcctgatc gttcccgggt tgggggacgc gttcgggatc ttcctgatcc
     1321 gccagttcgc cttcggcgtg ccggatgagc tcctggactc cgcgcggatc gacggcgcgg
     1381 gcgaggtccg gatcttcagc acgatcttcc ttcccctgtg cgcgccggcc gtgggcaccc
     1441 tcctggtcct gagcttcatg aaccggtgga acgacctctt ctggccgctc atcgtgacca
     1501 ccggcgacaa gcttcgcacc atccagctcg ggctgatcgc catgcagatg tccctctggg
     1561 agaccatctg gccgcagctc accgcctcca tggtgctggt cctgattccc attcttcttc
     1621 tctacatctt cctccaacga tacttcaccc agggcatcgt gctcacgggc ctgaaggggt
     1681 gaggcaagga gccgcaatgc ctgagctcga gaacgaggac atccgggtca ccctggacga
     1741 tgctggttcc ctggtgggtc tgtacgacaa gcgactgaag cgaagctacc tgggggcttc
     1801 gcagttctcc gggttgttcc gcctgatggt gccgagcccg cagtgggacg gacggcactt
     1861 cgactcgagg acgtccaagg gcatccgggt gcgcgcggtc tcggcaacgg aagcggaggt
     1921 cacctgcggg gagctcacct ccggcacgga ccggtgcgcc gccgcggtca ccgcacgcct
     1981 tcggctggac ggcgactgcc tcatcgcctc cctggagatc gagaatcagg gcgatgggtt
     2041 gatcaacgag gcgctcttcc cgttcatcgg cgggttcgcg acggacacgg gcgacctgac
     2101 cctgttcatc cccacgcagc atgcgcgtcg gatgatccga cccttccagg accttgggga
     2161 cgggaatcac aagggatgga cccgggagaa caaccggcac ttcatccgct accccacgca
     2221 cctggccacc gcgtgggtgg actactcctc cttccgcgga ggcgtgggga tcgaggcgcg
     2281 ctcccctcag ttcgaggtga ccgacttctg cttcgagcgc gccatccgca agagcccgcg
     2341 cgccgcggag gagaacgccc aggggctgta cgcggtgctc cagttccacc ccaacctggc
     2401 gcgcggcggc cgctggcgct cgccggacgt ggtggtgcgg ctgcatcgag gggactggca
     2461 cgtgaccgcg gacgcgcacc gcgagtggct ggagacggtg gtgcagccta ccgtgacgcc
     2521 ggaggcgttt cgcaacggcc tgggctggca cttctacttc atgaaggtcc aggacggcac
     2581 cttctttcgg acctacgacg atctgccggc catggccaag gcatcccagg cggcggggat
     2641 cgactacatc atggtgttcg gctggcagca gaagggacac gacaactact actccttcgg
     2701 ctactacccc aacgaggact ggggaggcgt agcgcgcctc aaagagaaga tccgcgaggt
     2761 ccgggcgcag ggctgccacg tgatcccgtt cttcaacggg accctcctgg acatcaccac
     2821 tccggagtac aaggagtacg ggcaccggtg gccggtgatc gggcggctgg gcagccccta
     2881 ctggggagga gacttttccc gagggaactt cgacttctgc ttccagaacg ccaacaacaa
     2941 cacgacggag cgcaacatga ccctcctgga cctctgcgtc accgggcggg aagcacggga
     3001 gtgggagaag gacaccatcc gccgcatctc ccaggagtac ggcttcggca acatccagct
     3061 cgatcagatc gcccacaagt gctacgtctg ctacgacccc gcgcacggcc actcggcgcc
     3121 gcagtacgca tccacgcggg agctcacggc cctgcttgcg gaggtgcgat ccgcgctccg
     3181 cgagcatgat ccggatggcg tgatggtcgg ggaagggttc accgacatga ccgcgcagta
     3241 ctgtgacggc ttctggagct ggtcccagct ccgcttcccc gagatcatcc gctacagcgt
     3301 gccatggatg gcgtactccc atgagaccga cgcgaacgag tacgcggagg tgaacacctg
     3361 cttcataaac cggatcctgc tggacctgaa gatcgagggc ggcgatggaa cgctggcgga
     3421 ctttccgcag ttcaccgctc acctccgccg actctccgat ctcaagaagc ggctggggcc
     3481 gacgtacatc gatggactct accgggacga ggaagggatc accgtggagg gcgataccgt
     3541 gcgggccaag gtctatcacg accgagcgcg caagacggcg tgcgtggtgg cggcaaacct
     3601 caccaaccgc cgcgcgtccg cgaccctgcg gctgagcgtg cccgtgcccg gagacatgcg
     3661 ggtggtgtcc tgggcgggcg gcgaggagag gacaggcgcc agatcgtcgt ttcggttgca
     3721 gctcgatcct tacgaagtgc gcgggtttga gttctcgatg cagcggtgac ggggaggagc
     3781 catgggtgtg acgttgaagg cgaaccagat cgggt
//
LOCUS       DASRZS010000679         9568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_754834, whole genome shotgun sequence.
ACCESSION   DASRZS010000679 DASRZS010000000
VERSION     DASRZS010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9568
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754834"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VFH83_16545"
     CDS             complement(<1..897)
                     /locus_tag="VFH83_16545"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HET6488039.1"
                     /translation="MTKVGLVKGTDRFRNVLEALRRAGDEVTERIHGRVLIKVNTVVE
                     DSPEPNTHVDALRAVLEFLRPLAPQRVVVGEASGNVLGGFRAFGFETLKDDYAVEFLD
                     FNTAGFEPLELLGVDGSPLPARLTAARRGFDCLISLSVPKAHSDAVVTLSGKNMMGFV
                     AGEEIWKIHGVKDFANHLEKQAVVIHQNLRRLLARVRPDVAVLDAFHSFEGGRVPQCG
                     EGTRVEPHLALAGADFVAVDAVAATALGFDPRDVGYLVYAALDGYGTADLSQIEISGT
                     PLSTARFPLRPALTLDRILGWKG"
     gene            complement(934..1728)
                     /locus_tag="VFH83_16550"
     CDS             complement(934..1728)
                     /locus_tag="VFH83_16550"
                     /inference="COORDINATES: protein motif:HMM:NF005556.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HET6488040.1"
                     /translation="MTIGKTVRMRSIFRASTGRTVIVAMDHGSIVGPVKGIERPAEVV
                     AACRAGGADCVLATRGVVQAAGDEWDRGLSFILRLTGGFTVLNRTFEELLISSPETAL
                     TYGACGVAVTVKFGHEREGESIRQASGVADACERLGLPLVVEALAKGKGMASNDPAAL
                     AVAARAAQELGADLVKTCYTGDPDSFRAVVEGCPAPVVILGGEKSDTLENVFSDVYHS
                     LQAGGKGIAMGRNIWQHERTQAIVEAMVGLVHEDWTIRAAVRHVGP"
     gene            complement(1725..3194)
                     /locus_tag="VFH83_16555"
     CDS             complement(1725..3194)
                     /locus_tag="VFH83_16555"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET6488041.1"
                     /translation="MLLVFDVGTTALKGALFTRGGQMHGRAERPLALIPSADPLVQEC
                     DALEWVSALREVVAELLGARADRSGGRIEGPIQAVVVSGNGPTLVAVDGSGQPVAPAM
                     TWMDRRGTEEAAIVSRKAGGPVDATFYLPKALWLFRNRPDLYERTTHFLPCPEFLSFL
                     MTGEALTFLPAPQFARYIWGPSLIAAVGMDPTRFPPFVSCGTKIGSVTETGEARLGIP
                     RGVPVVAGGPDFVVSLLGTATVRTGRACIRSGTSEGVNVVWPTAIADPRLLSLGHVIP
                     GLFNISGSISTSGKALEWFRRLTGETRLAYEEIFRRIEEVPPGARGLLFLPYLTGERA
                     PLWDASARGAFLGLSLSHGLPEMTRAVAESTGFAIRDVMEVMTEDGLSIRDLRITGTP
                     SRSPVWNQIKADITGHPILAPAILDSDLAGDASLALFALGDYPSLVDASEAIVSMGTT
                     YEPRSQYRSRYDELFSAYRQAYQGLKMIFPTLSATRRAE"
     gene            complement(3194..4219)
                     /locus_tag="VFH83_16560"
     CDS             complement(3194..4219)
                     /locus_tag="VFH83_16560"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET6488042.1"
                     /translation="MKAAVLTAAERIEVRELERPRVEAHQVLVKLKACGICTLEQRLF
                     TGHLRLGYPVVPGHEASGVVVEVGTSVYSGIPVGAHVALDLVARCGACHYCRTGRSNL
                     CLNRFSGRRSVLGGFGELVAVDASQVFSIPDHLAFDEAAFAEPLSCCLRSLRAIDLAL
                     AEDLLVLGAGPMGQLHVQLAARMGARVFVSDPDHSRLEMARSLGAFAAVDPSREDPSA
                     VVRTHTEGRGVDACVVTSPAPAALQTAFQAIAKGGRINIYTAYDDEPALPVDANTIHR
                     SEVRVLGTEGRTPQDFCQAVRLLSFGFVNVRPLISRKVGFGEIEAGMRAALASETYRV
                     LLEHESV"
     gene            complement(4268..5179)
                     /locus_tag="VFH83_16565"
     CDS             complement(4268..5179)
                     /locus_tag="VFH83_16565"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6488043.1"
                     /translation="MNESPYALPVVHSAVGIMTPLLLAAIGGLFTELAGMLNIALEGL
                     ILSGAFFSVILASATNSLALGILLGVACAMLLALLFGSVTLFLRANGFITGLATNLLA
                     SGLTIVLANQFFHTKGVIQFHNIPRLPVLTVPPALQRIPLVGDVLFGNNIIVYITWAI
                     VLVSTLIIYRSSFGLRLRATGLSPRTIVSLGLSPKSYQLAGILISGATCGMAGAMLSL
                     QLAAFVPESSAGRGWIALVAIYLGNKTPWGIVIAAFVFGMAESLSNYAQGVITVPADF
                     ILALPYVITVAAMILYAIYRHYRAPRA"
     gene            complement(5176..6219)
                     /locus_tag="VFH83_16570"
     CDS             complement(5176..6219)
                     /locus_tag="VFH83_16570"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6488044.1"
                     /translation="MNGKGRLADWLAGLRGLGVTLGLAFALAIACMLLLSREPLAAIY
                     SFFIGPFTNRYALGNMLNTAVPLVFTGLGISLAFKSSVYNLGGEGQVYAGGLAATAVC
                     LALPAANGYLGGLLALAASGVVSAILGGLSGFFRMRWNADEMISSFLISAAVILVVDY
                     CITGPLDDPSNNLLSTYAIGAQYQLLRIFPPSRLNGSAFIALAAAGMGWLFLYKSQAG
                     YEMRMCGLNREFARYGGINVSTYLVLPMVLSGGLHGLAGGLSVLGTYGASIKGFSSGM
                     GWNGIAVALIARNNPLGVLPASLLFAYLDAGARAATLGSDITYEIVAVIQSVIFYLVT
                     AQALWGLNRRRAA"
     gene            complement(6236..7783)
                     /locus_tag="VFH83_16575"
     CDS             complement(6236..7783)
                     /locus_tag="VFH83_16575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6488045.1"
                     /translation="MSYLEMRGITKRFPNGVVANNAVDFTVEANEIHAVIGENGAGKS
                     TLMKILHGLEQPDAGTITFRGRRVVLRTPLEANRQRIGMVHQHFRLIPDFTVAQNVVL
                     GQEPRRAGLFVDTSAAIERIAALIDSHGFSLDPRARVSQLTVGQMQQVEIIKMLYRDS
                     DLLILDEPTSVLTEQEVHRLFETMRSLKREGKTLIIITHKLAEVKRISDRVTVMRKGS
                     VVAVRETAAVDKSELSRLMIGREVLYQVDRDRRVCGGPVLELQEVGLKQRGRARPLLD
                     RVSLTVCACEIVGVAGVGGNGLKELEDVVTGLRRISSGRILQEGEDVSNLSSVGLRKR
                     GLAYVPADRMARGSSLESSVQENMIISQRHGFIRAGFLKGEEIQEFAERLTRSFSIDG
                     SPDSLLGTLSGGNIQKVILARELAAEAGLIVFSEPTWGLDVASSQFVYEKILEMRASG
                     AGILLISSNLDEVLGIADRIAVMYRGRLVSMRPNDRGLTKELIGEYMLGLRDDFAQAR
                     GAVGGGS"
     gene            complement(7827..8867)
                     /locus_tag="VFH83_16580"
     CDS             complement(7827..8867)
                     /locus_tag="VFH83_16580"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6488046.1"
                     /translation="MKRSFLAPAAALLCAVAVGPLFAAGAKESGTKGPVSLGIFVPGV
                     TAGSPLYEQMVGAAKKAAAANPKLSVKVVEGGFDQSKWTDQVMSMAATGEYAYILTSN
                     PSMPTVSLDVAKAYPKQRFLFTDAYYPDNPQFYTVMYNQVEQGYLDGYLAGLVTTSAM
                     KGANPDLKVGAIVAQEYPALTKEIMPGFQQGFQAVNPGITLDYRVVGNWYDANKAADL
                     ANSMIDAGADVILAIAGGAGQGVISAAQARGKYVLLFDSDQYQLAPGTIVGCAILREE
                     KLVTEILAKVVAGTVPFGTADVVDARSGYVDFADDDPLYAGAVPADVRAKMDGIVKKL
                     RTGALALPVPQM"
     gene            complement(9019..>9568)
                     /locus_tag="VFH83_16585"
     CDS             complement(9019..>9568)
                     /locus_tag="VFH83_16585"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET6488047.1"
                     /translation="PNASQIISVIVAEGGNPNAPSSVSGGEHAETPLHWAASNDDVAA
                     IDALLENGADIESPGAVFTNGSPLSDAVVFGQWQAARRLLGRGARPTFWQAAALGLLE
                     LVQERLAREPPPSSDEITNAFWNACRGGSRQTAELLLSRGADLNWVGHGGKTALAAAR
                     ESGRGALAAWLREIGARSTSEL"
BASE COUNT         1651 a         3214 c         3199 g         1504 t
ORIGIN      
        1 acccttccaa ccgaggatgc ggtccagggt cagcgcgggc ctgaggggga accgcgccgt
       61 cgaaaggggt gtcccggaga tctcgatctg ggacaggtcc gccgttccat acccatccag
      121 ggcagcgtac accaggtacc cgacgtcccg gggatcgaac cccagcgcgg tggcagccac
      181 ggcatccacg gccacgaagt cggctcccgc gagcgccagg tgcggttcca cgcgggtacc
      241 ctccccgcac tgcgggacgc ggcctccctc gaaggaatgg aacgcgtcca ggaccgccac
      301 atcgggacgc acccgcgcca gaagacggcg gaggttctga tggatcacca cggcctgctt
      361 ctccagatgg ttcgcgaagt ccttcacgcc gtggatcttc cagatctcct ctccggccac
      421 gaagcccatc atgttcttgc cggacagggt gaccaccgca tcgctgtggg ccttggggac
      481 ggacagcgag atgaggcagt cgaaaccccg tcgggccgcg gtcagtcgcg cgggcaaggg
      541 gctgccatca acgcccaaga gctccagagg ctcgaagccg gccgtgttga agtccaggaa
      601 ctccaccgcg taatcgtcct tcagggtttc gaagccgaac gcgcggaagc cacccaggac
      661 gtttccgctc gcctccccga cgaccacccg ctggggcgcc agaggccgca ggaactccag
      721 gacagctctg agggcgtcca cgtgggtgtt gggctccgga ctatcctcca ccactgtgtt
      781 cactttgatg agcacccggc cgtggatgcg ctcggtcact tcgtccccgg ctcgtcgaag
      841 cgcctccagc acattccgga accgatcggt ccccttcacc aatccaacct ttgtcatcgg
      901 acctccctcc tgctcgtcct gacgccgtcg gagctacggg ccgacgtgtc ggaccgcggc
      961 gcgaatggtc cagtcttcat ggaccagccc caccatggcc tccacgatag cctgagtccg
     1021 ttcgtgctgc cagatgttgc gtcccattgc gatgcccttc ccacccgcct gaagggagtg
     1081 gtagacgtcg gagaagacgt tctccagggt atcgctcttc tcgccgccca ggatcacgac
     1141 gggagccggg cagccctcca ccacggcgcg aaagctgtcg ggatcgcccg tgtaacaggt
     1201 cttcacgagg tccgcgccca gctcctgggc agctcgggct gccacggcga gggctgccgg
     1261 atcgttgctc gccattccct tacccttggc cagggcttcc accacgaggg gaagacccag
     1321 ccgctcgcac gcgtccgcaa ccccggatgc ctgacggatg gactctcctt cccgctcgtg
     1381 gccgaacttc acggtcaccg ccacgccgca cgccccgtag gtaagggccg tctcggggct
     1441 ggagatgagc agctcctcaa aggtcctgtt caggacggtg aagccgccgg tcagtcgcag
     1501 gatgaacgag agcccgcgat cccactcgtc tccagcggcc tgaacgaccc cacgggtggc
     1561 caggacgcag tccgcgcccc cggcccgaca ggcggccacg acctccgccg gacgttcgat
     1621 gcccttcacc ggtcccacga tgctgccgtg gtccatcgcc acgatcacgg tcctccccgt
     1681 ggaggcgcgg aagatcgatc tcattcgtac ggtcttgccg atggtcattc cgctctcctt
     1741 gtcgccgaca gggtcgggaa tatcatcttc aggccctggt aggcctgccg ataggccgag
     1801 aagagctcgt cgtagcgcga gcggtattgg gagcggggct cgtaggtggt gcccatggaa
     1861 acgatggcct ccgaggcatc caccagagaa gggtagtccc cgagagcgaa aagcgccagg
     1921 ctggcgtccc ccgccaggtc ggagtccagg atggccggcg ccaggatagg atgtccggtg
     1981 atgtcggcct tgatctggtt ccagaccggg cttcgggacg gggtgccggt gatccgcagg
     2041 tcccggatgc tgaggccgtc ctctgtcatt acttccatca cgtctcgaat ggcgaacccc
     2101 gtggattccg ccacggctcg cgtcatctcc gggagaccgt ggctcaagct cagccccagg
     2161 aacgctcccc gggcactggc atcccagagc ggtgcccgtt ctccggtgag gtagggcagg
     2221 aagagaagcc cgcgcgcgcc cggcggcact tcctcgatcc gacgaaagat ctcctcgtag
     2281 gccaggcggg tctcaccggt gagccggcgg aaccactcca gcgccttccc cgaggtggag
     2341 atcgagccgg agatgttgaa gagccccggg atcacgtgcc ccagggagag cagccgcggg
     2401 tctgcaatgg ccgtgggcca gaccacgttc accccctcgg aggtcccgga gcggatgcag
     2461 gcccggcccg ttcgaacggt ggcggtcccc aggagggaga cgacgaagtc cgggccaccg
     2521 gcgaccaccg ggacgccacg agggatgccc agccgggctt ccccggtctc ggtgaccgaa
     2581 ccgatcttcg ttccacacga aacgaaggga ggaaaccgcg tcggatccat gcccacggca
     2641 gcgatcaagg aaggccccca gatatagcgg gcgaactggg gcgcaggcag aaacgtaagc
     2701 gcctcacccg tcatcaggaa ggagaggaac tccgggcacg gcagaaagtg ggtcgttctc
     2761 tcgtagaggt cggggcggtt tcgaaagagc cagagggctt tgggaaggta gaacgtcgca
     2821 tcgacggggc cgccggcctt gcgggagacg atggcggcct cctccgtgcc gcgtcgatcc
     2881 atccacgtca tggcgggggc cacgggctgt ccgctgccgt cgacggccac cagcgtcggc
     2941 ccgttgccgc tcaccacgac agcctggatc ggcccttcga tgcgtccgcc cgaccgatcg
     3001 gctctggccc ccaggagctc ggcgaccacc tcccgaaggg cggaaaccca ctcgagggcg
     3061 tcgcactcct gcaccagggg gtccgcgctt ggaatcaggg caaggggacg ctccgcccgg
     3121 ccgtgcattt ggccgccccg ggtgaaaaga gctcccttga gcgcggtggt tcctacgtcg
     3181 aagaccagga gcatcagacg ctctcgtgtt ccagaagcac ccggtaggtt tcggaagcta
     3241 gcgccgcgcg cataccggcc tcgatctccc caaatcccac cttgcggctg atcagcggcc
     3301 gtacgttcac gaagccgaac gagagaagcc ggaccgcctg gcagaagtcc tggggcgtcc
     3361 ttccttcggt gcccaggacg cggacctccg aacggtggat cgtgttggca tccacgggga
     3421 gggcgggctc atcgtcatac gcggtgtaga tgttgattcg ccctcccttg gcgattgcct
     3481 ggaaggcggt ctggagcgcc gcaggcgccg ggctggtcac gacgcaggca tccacgcccc
     3541 tgccctccgt gtgcgtccgg accacggctg acggatcctc ccggctcggg tcgacggccg
     3601 caaaggcccc gagggagcgg gccatttcca ggcgggagtg gtccgggtca ctcacaaaga
     3661 ctcgggcgcc cattcgcgcc gccagctgga cgtgaagctg tcccatgggc ccggctccca
     3721 gcaccagaag gtcctcggcc agggcgaggt cgatggcgcg cagggaacgg aggcagcagg
     3781 agagcggctc ggcgaaggct gcctcgtcga acgcaaggtg gtccgggatg gagaacacct
     3841 gcgaggcatc caccgccacc agctcaccga acccgcccag gacggaccgc ctgccgctga
     3901 accggttcag gcagaggttg gatcggccgg tgcggcagta gtgacacgct ccgcatcggg
     3961 ccaccaggtc cagggccacg tgcgccccaa cggggatgcc gctgtagacc gaggtcccga
     4021 cttccactac gactccggat gcctcgtgac cgggcaccac ggggtatccg agtcgcaggt
     4081 gtcccgtaaa cagccgctgc tccagcgtac agatcccgca ggccttgagc ttcaccagga
     4141 cctggtgcgc ctcgacgcgg ggccgctcca gctcccgaac ctcgatccgc tccgccgccg
     4201 tcaggactgc tgccttcatg gcgctctcct tccgagccga ccgcctcagg tcctcaggcc
     4261 cgcggcgcta agcccgcggg gcccgatagt ggcggtagat cgcgtagagg atcatggccg
     4321 ccacggtgat gacgtagggg agcgccagga tgaagtccgc gggaacggtg atgaccccct
     4381 gggcgtagtt ggagagactc tccgccatcc cgaacacaaa cgccgcaatg acgatgcccc
     4441 atggcgtctt gtttcccagg tagatggcca ccagcgcgat ccagccccgg cccgcggagc
     4501 tctcgggcac aaacgcggcc agttgaaggc tcagcatggc gcccgccatg ccgcaggtgg
     4561 cgccggagat gaggatgccg gccaactggt aggacttggg gctcaacccc agggacacga
     4621 tggtgcgggg gctgagcccg gtggcgcgca gccgcagtcc gaaggaggac cggtagatga
     4681 tgagcgtgga caccagcacg atggcccagg tgatgtagac gatgatgttg ttcccgaaaa
     4741 gcacgtcccc gaccaggggg atgcgctgca gggccggagg caccgtcagg acgggcagac
     4801 gggggatgtt gtggaactgg atcacgccct tggtgtggaa gaactggttg gccagaacga
     4861 tggtcagccc ggaggcaagg aggttggtgg caagccccgt gatgaacccg tttgcccgaa
     4921 ggaacagggt gacggagccg aagagcagcg ccaggagcat cgcgcaggcc acacccagga
     4981 ggatccccag cgccaggctg ttggtggcgg aggccaggat cacgctgaag aaggcaccgg
     5041 agaggatcaa gccctccagc gcgatgttga gcattccggc aagctcggtg aacaggcccc
     5101 cgattgccgc gagcagcaag ggggtcatga ttcccacggc gctgtggacc acgggcaggg
     5161 cataggggga ttcgttcacg cggccctccg acggttcagc ccccagaggg cctgggcggt
     5221 caccaggtag aagatcacgg actggatcac ggcgacgatc tcgtacgtga tgtcggagcc
     5281 caaggttgcc gcccgagcac cggcgtccag gtaggcgaac aggagcgagg ctggcaggac
     5341 gccaagagga ttgttgcgcg cgatgagcgc gaccgcgatc ccgttccacc ccatcccgga
     5401 ggagaagccc ttgatgctcg cgccgtaggt cccgaggacc gacaggccgc cggccagacc
     5461 gtgcagcccg ccgctcagga ccatcgggag cacgagatag gtggaaacgt tgatcccacc
     5521 gtagcgagcg aactcccggt tcagtccgca catgcgcatc tcgtagccgg cctggctctt
     5581 gtagaggaag agccacccca tgccggccgc ggccagggca atgaaggcgc tcccgttgag
     5641 cctggagggt gggaagatcc gcaggagctg gtactgggcg ccgatggcgt aggtcgacag
     5701 gagattgttg gacgggtcgt ccaggggacc cgtgatgcag tagtccacca ccaggatcac
     5761 ggcggcggag atcaggaagg aggagatcat ctcatcggcg ttccagcgca tccgaaagaa
     5821 cccggacagg ccgccgagga tcgccgagac gaccccggag gcggcgagcg ccaggagacc
     5881 gcccaggtat ccgtttgccg cgggcaacgc caggcacacg gcggtggccg ccaggcctcc
     5941 ggcgtacacc tggccctcgc cacccaggtt gtacacggag gacttgaagg cgaggctgat
     6001 cccaagaccg gtgaacacca gggggacggc ggtgttcagc atgttgccca gggcgtatcg
     6061 gttggtgaac ggtccgatga aaaaggaata gatggcggcg agcggctccc ggctgaggag
     6121 gagcatgcac gcaatcgcga gggcgaacgc caggcccagg gtgacgccga gccctcgaag
     6181 cccggccagc cagtccgcga gcctcccttt cccgttcacg gcgcccctcc gacggtcacg
     6241 aaccgcctcc gaccgcgccg cgggcctggg cgaagtcgtc ccgcagcccg agcatgtact
     6301 cgccgatgag ctccttggtg agcccccggt cgttcggacg catggagacc aggcggcctc
     6361 ggtacatcac ggcgatgcgg tccgctatgc ccagcacctc gtccaggttg gaggagatca
     6421 gcaggatccc ggcgccggag gcgcgcatct ccaggatctt ctcgtagacg aactggctgc
     6481 tggcaacgtc cagaccccag gtgggctcgg agaacacgat gagcccggcc tccgccgcaa
     6541 gctcccgagc caggatgacc ttctggatgt tcccgccgga gagggtgccc aggagcgagt
     6601 cgggggaccc gtcgatggag aagctccggg tgagccgctc ggcgaactcc tggatctcct
     6661 cgcccttgag aaaccccgcc cggatgaatc cgtggcgctg gctgatgatc atgttctcct
     6721 ggacgctgga ctccaggctg gagccacgcg ccatgcggtc cgcggggacg taggccaggc
     6781 cgcgctttcg aaggcctacg gaggaaaggt tggagacgtc ctccccctcc tggaggatgc
     6841 gtcccgagct gatgcggcga agtccggtga ctacgtcctc cagctccttg agtccgttgc
     6901 cgcccacccc ggcaacgccg acgatctcac aggcgcacac ggtcaggctc acgcggtcca
     6961 ggagcggccg cgcgcggccg cgctgtttca ggcctacctc ctggagctcc aggacgggac
     7021 caccacagac ccgtcggtcc cgatccacct ggtagagcac ctcgcggccg atcatgagcc
     7081 gcgaaagctc gctcttgtcc accgccgcgg tctcccgcac cgccaccacg gagcctttcc
     7141 gcatcacggt gacccgatcc gagatccgct tcacctcggc cagcttgtgg gtgatgatga
     7201 tgagggtctt gccttcccgc ttcaggctgc gcatggtctc gaacagccgg tgcacctcct
     7261 gctccgtcag caccgaggtc ggctcgtcca ggatgagcag gtcggagtcg cggtagagca
     7321 tcttgatgat ctccacctgc tgcatctggc ccacggtgag ctgggagacc cgggcccggg
     7381 ggtccaggct gaacccgtgg ctgtcgatga gcgccgcgat gcgctcgatc gcggccgacg
     7441 tatccacgaa gaggccggcc cgccgcggct cctgtcccag caccacgttc tgagccacgg
     7501 tgaaatcggg aatcaggcgg aagtgctggt gcaccatccc gatcctttgg cggttggcct
     7561 ccagcggcgt gcgcaggacc acccgacggc cgcggaaggt gatggtcccc gcatccggtt
     7621 gctccagacc gtggaggatc ttcatgaggg tgcttttgcc ggccccgttc tctccgatca
     7681 ccgcgtggat ttcgttggcc tccacggtga agtctacggc gttgttggcc acgacgccgt
     7741 tggggaaccg tttggtgatg cccctcatct ccagatagga catgctctcc tcggggggac
     7801 ggctggaaag accgcggcgg gctccgtcac atctggggga cgggaagggc tagggcgccg
     7861 gtccgcaact tcttcacgat cccgtccatc ttcgcccgga cgtccgccgg cacggcgccg
     7921 gcgtacaggg gatcgtcatc ggcgaagtcg acgtagccgc tcctggcatc gacaacgtcg
     7981 gccgtgccga aggggacggt gccggccacg accttggcca ggatctccgt caccagcttc
     8041 tcctcgcgca ggatggcgca tccgacgatg gtccctggag cgagctggta ctggtcgctg
     8101 tcgaagagga gcacgtactt gccgcgggcc tgggccgcgc tgatgacgcc ctggccagcc
     8161 ccgccggcga tcgccaggat cacgtccgct ccggcgtcga tcatgctgtt ggcgaggtcg
     8221 gccgccttgt tggcgtcgta ccagttgccg accacccggt agtccagcgt gatgccgggg
     8281 ttcaccgcct gaaagccttg ctggaagccc ggcatgatct ccttggtgag cgccgggtat
     8341 tcctgggcca cgatggctcc gaccttcagg tcagggttgg cgcccttcat ggcgctggta
     8401 gtcaccaggc cggcaaggta gccgtccaga tagccctgct ccacctggtt gtacatcacg
     8461 gtgtagaact ggggattgtc gggatagtag gcatccgtga agaggaacct ctgcttggga
     8521 tatgccttgg ccacgtccag gctcaccgtc ggcatggacg ggttggatgt caggatgtag
     8581 gcgtattccc cggtagccgc catggacatg acctggtcgg tccacttgga ctgatcgaac
     8641 ccgccttcca cgaccttcac cgacagcttg gggttggccg cggcggcttt cttggccgcg
     8701 cctaccatct gctcgtacag ggggctgccc gccgtgactc cgggaacgaa gatcccaagg
     8761 ctcaccggcc ccttcgtccc ggactctttc gcgccggccg cgaagagcgg cccgacggct
     8821 accgcgcaga gcagcgccgc cgccggagcc agaaacgatc gcttcatagg gcacctcctt
     8881 cgttgggtgt ggccaacacc gcaggactca ccatactcac gacgggcatc ctcttctacc
     8941 ggtcgccgcc ggtgttgccg gtggctccgg gagctgccgg tcgctccagg cggcgtccgt
     9001 agcccggagg tggtctgact aaagctcgct cgttgaccgg gcgccgatct cccgcagcca
     9061 ggcggccagg gcgccgcgac cgctttcccg ggccgccgcc agggccgtct tcccgccgtg
     9121 tccgacccag ttgagatcgg cgccccgtga gagcaggagc tcggcggtct ggcggctccc
     9181 tccgcggcag gcgttccaaa aggcgttggt tatctcgtcg gaggatggcg gcggctcgcg
     9241 agccagccgc tcttgaacca gttcgaggag tccgagagcc gcggcctgcc agaatgtcgg
     9301 gcgggccccc cgccccagca gcctccgcgc ggcctgccat tgaccgaaca ccaccgcgtc
     9361 cgacaggggg gaaccgttgg tgaagaccgc gccaggggac tcgatatcgg caccattctc
     9421 caggagagcg tcgatggcag ctacatcgtc gttgctcgcc gcccagtgca ggggcgtctc
     9481 cgcgtgctca cctccggaga cgctggaggg ggcgttcgga ttcccgcctt ccgctacgat
     9541 gacggagatg atctgcgacg cgttgggc
//
LOCUS       DASRZS010000680         4313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_5385755, whole genome shotgun sequence.
ACCESSION   DASRZS010000680 DASRZS010000000
VERSION     DASRZS010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5385755"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1091
                     /locus_tag="VFH83_16590"
     CDS             117..1091
                     /locus_tag="VFH83_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019637201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6488048.1"
                     /translation="MAAPVNAPASLPVEPLRSPADRGFFFELRKNKLLFLMLLPTFLY
                     FLLFFYIPFVGAYYAFVDYKYQLGLFRSPFVGFRYFRFLFLGNIITKLTINTLLYNVA
                     FIIIGNFFQITVAILISQITSPFFKRLAQTFIFLPYFISFVLVGLFAYGLLNYDYGII
                     NHALEALGLKAVAFYTAPQYWKYILTIAHVWKWLGYGSVIYLAAIMSINNELYDVAAI
                     DGANAFRQITAVTLPMLRPTIIILLLFSIGGILRGQFELFYQMVGRNGQLYNATDIID
                     TYVYRALTVTAQIQLGTAAGLFQSVFGLAVILGANFIIKRIDPEYALF"
     gene            1101..1997
                     /locus_tag="VFH83_16595"
     CDS             1101..1997
                     /locus_tag="VFH83_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013372217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6488049.1"
                     /translation="MAVKKGPDYTLLRIIAYVVVSLAAIACLLPFLMLISSSFTKESE
                     IVRHGFSLLPLKFSLEAYTFAFEVPKRIIDAYLVTITVTAVGTFFSLFFVSMAGYVLQ
                     RKDFKYRNRISFIIYFTQLFSVGIIPQYILITRFLGLTDSLLALILPPLLSPFLIILM
                     RTFIKTAVPDSIVESAKIDGASDFLIYRKLILPLSTAGLATIGLFTALGYWNDWFQGS
                     LYIRTTSRYPLQFYLYNLLMSAQMIREMANVAMSTNSTAVLPQESFKMATALIVTGPI
                     LLLYPFVQRYFVSGLTIGAVKG"
     gene            2004..2471
                     /locus_tag="VFH83_16600"
     CDS             2004..2471
                     /locus_tag="VFH83_16600"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HET6488050.1"
                     /translation="MEPSKRIALVAHDNMKQDLIQWVEYNYATLLRHTLICTGTTGRL
                     VERAIRRKAGDTLPTDFSVRALKSGPLGGDQQLGALIAEGGVDCVIFFWDPMSPHPHD
                     VDVKALLRIAVLYNIPIACNRSTADFIISSSIMEKPYQPVVKDYGSYIERTLP"
     gene            2582..4231
                     /locus_tag="VFH83_16605"
     CDS             2582..4231
                     /locus_tag="VFH83_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HET6488051.1"
                     /translation="MSNLIWKTESAYDYPLLIKNLFAAPIVDNPDQEIVYRGSLRLTY
                     RTFRDRVARLAAALQALGISAGDTVAVMDWDSHRYLECFYAVPMMGAVLHTVNVRLSA
                     EQLIYTISHAEDTLIIVNSEFLPLLEQIKSRIDTVKKLVLIVEGGGEPPASSLRFVGE
                     YETLLAAAQPLAEFPDFDENTRATTFYSTGTTGMPKGVYFSHRQLVLHTLGTLGTLSS
                     AAAGARFHREDVYMPLTPMFHVHGWGIPYVATMLGVKQVYPGKYVPEILLKLLVEEKV
                     TFSHCVPTILNMLVSNPAVARFDLSRWKVIIGGAALPKSVAVAALKRGIDVFVGYGLS
                     ESGPVLTVSQPTTAELALPPEQQVELRCRTGVPFAMVQLKVVGDDGTEVSRDDRTSGE
                     ILVRAPWLSQGYLKDHSNSEKLWAGGWMHTLDVAARNAAGSIRITDRAKDIIKVGGEW
                     LSSLELEDILALHPSVAEAAVIGQQDVTWGEIPVALVVLKKDAKAAEKEISTHVKNYV
                     DQGVLPREAYLVKVRFVDSIDKTSVGKINKVALRAKHLSAT"
BASE COUNT          783 a         1428 c         1254 g          848 t
ORIGIN      
        1 tggacatccg accggtcgtg gtgcaatagg aggagcgggc cgcccgggcc gcgcggcggt
       61 ttattcttga caccggcgcg cggctgactt atagtgcggc ccacacggaa gcacgaatgg
      121 ctgcacctgt gaacgcgcca gcaagtctgc ctgtggagcc gcttcgctcg cccgccgatc
      181 gcggcttctt cttcgaacta cggaagaaca agctgctctt cctgatgctg ctcccgacgt
      241 tcctgtactt cctgctcttc ttctacattc cgttcgtggg cgcctactac gcgttcgtcg
      301 actacaagta ccagctcggg ctgttccgca gcccgttcgt gggattccgg tatttccggt
      361 tcctcttcct cggcaacatc atcaccaagc tcacgatcaa cacgctgctc tacaacgtgg
      421 cgttcatcat catcgggaac ttcttccaga taacggttgc catcctgatc agccagatca
      481 ccagcccgtt cttcaagcgg ctcgcgcaga cgttcatctt ccttccctac ttcatctcct
      541 tcgtgctggt cggcctcttc gcgtatgggc tcctcaacta cgactacggc atcatcaacc
      601 acgccctgga ggcgctggga ctgaaagcgg tcgccttcta caccgcgccg cagtattgga
      661 agtacatcct gaccatcgcg cacgtctgga agtggctggg gtacgggtcc gtcatctatc
      721 tggcggccat catgtcgatc aacaacgagc tgtacgacgt ggcggccatc gacggagcca
      781 acgcgttccg ccagatcacg gcggtcacgc tgcccatgct gagacccacg atcatcatcc
      841 tgctgctctt cagcatcggc gggatcctga gggggcagtt cgagctcttc tatcagatgg
      901 tcgggcgcaa cggccagctc tacaacgcca cggacatcat tgacacctat gtctatcggg
      961 ccctcacggt gaccgcgcag attcagctcg ggaccgccgc gggtctcttc caatcggtgt
     1021 tcggactggc ggtcatcctc ggcgcgaact tcatcatcaa gcggatcgat cccgaatatg
     1081 cgctgttcta gggggaggcc atggcggtaa agaaggggcc agactacacc ctgcttcgga
     1141 tcatcgcgta cgtcgtggtc agcctcgcgg ctattgcgtg tctgctgccg ttcctcatgc
     1201 tgatctcctc ctcgttcacc aaggagtcgg agatcgtccg ccatggattc tcgcttctgc
     1261 cgctgaagtt ttccctggag gcgtacacgt tcgcgttcga ggtccccaaa cggatcatcg
     1321 atgcgtatct ggtgaccatc acggtgaccg cggtgggcac gttcttctct ctgttcttcg
     1381 tttccatggc gggatacgtc cttcaacgaa aggacttcaa gtatcgcaac cgcatctcct
     1441 tcatcatcta ttttacccag ctgttctccg tggggatcat cccgcagtac atcctgatca
     1501 cgaggttcct cggcctgacc gacagcctcc tggcgctgat cctcccgccg ctcctcagcc
     1561 cgtttctgat cattctcatg cggaccttca tcaagacggc ggtcccggat tccatcgtgg
     1621 agtccgccaa gatcgacggg gccagtgact tcctgatcta ccgcaagctc atccttccgc
     1681 tctccacggc aggactggcc accatcggcc tcttcaccgc cctgggctac tggaacgact
     1741 ggttccaggg cagcctctac attcgcacca cgagccggta cccgcttcag ttctacctct
     1801 acaacctgct catgagcgca cagatgatcc gggagatggc caacgtggcc atgagcacga
     1861 actccaccgc ggtcctgccg caggaatcgt tcaagatggc gaccgccttg atcgtcaccg
     1921 gtccgatcct gctcctgtac ccgttcgtcc agcgatactt cgtgagcgga ctgaccatcg
     1981 gcgccgtcaa aggataggga cgggtggagc cctccaagcg aatcgccctc gtcgcccacg
     2041 acaacatgaa gcaggacctc atccagtggg tggagtacaa ctacgccacg ctcctgcgcc
     2101 acaccctgat ctgcaccggt accacgggcc ggctggtgga gcgggccata cggcggaagg
     2161 ccggtgacac cctgccgacg gacttctcgg tccgcgcgct gaagtccggt ccgctgggcg
     2221 gtgaccagca gctaggcgcg ctcatcgccg agggcggcgt ggactgcgtc atcttcttct
     2281 gggacccgat gtccccgcac ccgcatgacg tggacgtcaa ggcgctcctg cggatcgcgg
     2341 tcctctacaa catccccatc gcctgcaacc gatccaccgc ggactttatc atctcctcat
     2401 ccatcatgga gaagccctac cagcccgtgg tgaaggacta cggaagctac atcgagcgga
     2461 ccctcccgtg accggcttcg ctcggcaatc ccggtagtgc cgcggggtag cggcagcggg
     2521 gcctctacgg ttattatctg acttcgaaac tcatccatag tcagcgttga cggaggcatc
     2581 catgagcaat ctcatctgga agaccgaatc cgcctacgac tatcccttac tgatcaagaa
     2641 ccttttcgct gcccctatcg tcgacaaccc ggaccaggag atcgtctacc gcgggtcgct
     2701 tcgtctcacc taccgaacgt tccgcgaccg tgtggcccgg ctggccgccg ccttgcaggc
     2761 gctggggatc tcagcgggag acacggtggc ggtcatggac tgggacagcc accgatacct
     2821 ggagtgcttc tatgccgtgc cgatgatggg ggcggtcctg cacacggtga acgttcgcct
     2881 ctccgcggag cagctcatct acacgatctc ccacgcggag gacacgctta tcatcgtcaa
     2941 ctccgagttc ctcccgctgc tagagcagat caagagccgg atcgacaccg tcaagaagct
     3001 ggtgctcatc gtggaagggg gcggcgaacc accggcgtcc tccctccgct ttgtcggaga
     3061 atacgagacc cttctggcgg ccgcgcagcc gctggcggag ttccccgact tcgacgagaa
     3121 cacccgcgca accaccttct actccacggg gaccacgggc atgcccaagg gggtgtactt
     3181 cagccatcgg cagctcgtcc tgcacaccct ggggactctc ggaacgttga gctccgcggc
     3241 ggcgggggcg cgcttccatc gggaggacgt atacatgccg ctcacgccga tgttccacgt
     3301 ccatggctgg ggcatcccgt acgtggcgac gatgctgggg gtcaagcagg tgtatccggg
     3361 caagtacgtg cccgagatcc tcctgaagct gctcgtagaa gagaaggtga cgttctccca
     3421 ctgcgtgccc accatcctga acatgctggt cagcaatccg gcggtcgccc ggttcgatct
     3481 ctcgcgatgg aaggtgatca tcggcggcgc ggcgctcccc aagtccgtag ccgttgccgc
     3541 gctcaagcgg ggcatcgacg tcttcgtggg gtacgggctg tcggaatccg ggcccgtcct
     3601 caccgtctcc cagcccacca ccgcggagct tgccctgccc ccggagcagc aggtggagct
     3661 gcggtgtcga acgggagtgc cgttcgccat ggttcagctc aaggtggttg gcgatgacgg
     3721 aacggaggtc tctcgggacg acaggacctc cggagagatc ctggtgcggg ccccctggct
     3781 cagccagggc tacctcaagg accactccaa ctcggagaag ctatgggccg gcgggtggat
     3841 gcacacgctg gacgtggcgg ctcgcaacgc ggcgggctcc atccggatca ccgatcgggc
     3901 caaggacatc atcaaggtcg gcggggaatg gctgtcctcc ttggaactgg aggacatcct
     3961 ggcgctgcac ccgtccgttg cggaggccgc cgtgatcggg caacaggacg tcacgtgggg
     4021 agagatcccg gtggcactgg tggtcctcaa gaaggatgcg aaggccgccg agaaggagat
     4081 ctcgacgcac gtcaagaact acgtcgacca gggcgtcctt ccccgggaag cctacctcgt
     4141 caaggtgagg ttcgtagatt ccatcgacaa gaccagcgta gggaagatca acaaggtagc
     4201 cctgcgggcg aagcacctca gcgcgacgta gcagccgtag gtccctacgc cgggagccgg
     4261 gcgagcgaac gccagcctct attccagcgg ccgccaggcc gaccgctcca gct
//
LOCUS       DASRZS010000681         4746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4598569, whole genome shotgun sequence.
ACCESSION   DASRZS010000681 DASRZS010000000
VERSION     DASRZS010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4598569"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..894)
                     /locus_tag="VFH83_16610"
     CDS             complement(16..894)
                     /locus_tag="VFH83_16610"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET6488052.1"
                     /translation="MPQSAPSFSKYLAATARDIEWGVYCTTVGYTKMPTAPRYPPEPK
                     AHPPKYLFQWEMGRIYDEYALVYITRGKGTFRSDGDRSWRVGPGTLFMLFPEVWHWYA
                     PDKETGWDEYWVGLKGDYIGRLVEKGFFSPESPVMEIGLHDSILELFQEIIDTARYEG
                     PGYQQVLGSLITHLLAHVHSLTLEHGRDNSNERIVQRAKFLLADNVTAHLDIGWLSKT
                     LGLGYSRFRQVFKKCTGLSPYQYFLELKINRAKELLQQGQFTVKEIAYMLSFDDPYYF
                     SHLFKKKTGHPPSAWH"
     gene            complement(917..2146)
                     /locus_tag="VFH83_16615"
     CDS             complement(917..2146)
                     /locus_tag="VFH83_16615"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6488053.1"
                     /translation="MTVRERFREVMAGGKAAAPKWEFGYWGANYANWYREGLPRHRPP
                     RPLTRTTTSASSLYIPCWNALPKGELAAGMAVLGGGLYWPTQGFALDHDVQRACAMDP
                     GQVLVNVDLLFHPRFEPRVIEETDTHMIYVDIDGVKRQFEKVTGVIPMALENPIRDWA
                     SWNALKRERLSTKDIAGRFPPHWKDLLRRYRDRDYPLVLGGYPCGYFGTLAHLMGYEH
                     LFYNYADEPGLIHDINATFTEIWLAVYEEVLAQTDVDLFVIWEDISAGTGSMVAPPTI
                     REFMLPYYQRLTGFLKAHGVKTIFVDTDGECTALIPFFLEAGVTGLYPMEASCGVDIV
                     KVRKSFPTLQLMGGIPKSEIVKGAARIEQILEPVRQVLATGRYVPFGDHLIPPEVHWP
                     EFSRYRRRLNELIDSVN"
     gene            complement(2143..3141)
                     /locus_tag="VFH83_16620"
     CDS             complement(2143..3141)
                     /locus_tag="VFH83_16620"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6488054.1"
                     /translation="MANTSTMTAPSPGGIRALYGRVAAMGSTLSLAIALFLLCVVWAS
                     LSPYFLTLQNIMNVLMFASILAIRASGLTVSMILGGMDISQNATGAVTALLCALVAAA
                     GAPWWIVLILVILLALSMGLINATLVSVFKIAAIITTLGTMQIFRGAAWLIKDATVMV
                     TDPVILSLGRGRLFGIIPYVTIIAVVVLAIVYYLLNFTSFGRKVYMVGGNERASYLSG
                     INVNRVKFASFVISGLAAGISGFLLAGQVGAALPQSGAGTEMNTIAAVILGGISLSGG
                     KGKLSGTILGILILQTINNGLTLLSINAYWQMVVSGGVLLLAVMIDVVRSGALKKQ"
     gene            complement(3134..4657)
                     /locus_tag="VFH83_16625"
     CDS             complement(3134..4657)
                     /locus_tag="VFH83_16625"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6488055.1"
                     /translation="MSSTVGERSEYSLQVKNVVKRFPGVLALDGVDLEIRPGEVLGVI
                     GENGAGKSTLMKILAGAYFMDTGEILLDGTSIPPESNPKERMALGLGIIYQELNYLNE
                     MTIAENFFMGRVPVKGFLRRVDYAAMKKASRELLAIFNLDYNPLTLVKTLSVAEKQML
                     EILRAVSRDVKVLIMDEPTSSLNEVETERLFGCIEDLRKRGVSVIYITHKLEEVLRLA
                     DRIMVMRDGKRVGVVSKGETTSGKLVEMMVGRKIAEMYPKEQLSAGAEVLRVEHLSCG
                     CATDISLSVHKGEILGLYGLVGSGCVETVEGLLGIRARTRGTIVLDGAEVRIDDPLAA
                     KRLGIAYVPSDRKQEGLVLIHSLKDNVAVTKLDELGRGLKLNGRKMKRYAEAWIAKLQ
                     IRAPSANAIVESLSGGNQQKVVVAKWLLTDPKVLIMNDPTRGVDVGAKVEIYKIMEEL
                     CRGGISIIMVTAELPEAIGITDRMLVLANGRVVGEYRRSEYDQGTILHLAVKGNGNG"
BASE COUNT          808 a         1525 c         1554 g          859 t
ORIGIN      
        1 gatcgacgcg accgctcagt gccaggcgga aggcgggtgg ccggtcttct tcttgaacag
       61 gtgagagaaa tagtaaggat cgtcgaagga gagcatgtag gcgatctcct tgaccgtgaa
      121 ctgcccctgc tgcaggagct ccttggcccg gttgatcttg agctccagga agtactggta
      181 gggggagagc cccgtgcact tcttgaacac ctggcggaat cgcgagtagc ccaggcccag
      241 ggtcttcgag agccagccga tgtccaggtg agccgtgacg ttgtcggcca ggaggaactt
      301 cgcgcgctgg acaatgcgct cgttgctgtt gtcccgaccg tgctcgaggg tgaggctgtg
      361 cacgtgggcc aggaggtggg tgatcagcga ccccagcacc tgctgatagc ccggtccctc
      421 gtagcgcgcc gtgtcgatga tctcctgaaa cagctccagg atggaatcgt ggagcccgat
      481 ctccatgacc ggggactccg ggctgaagaa ccccttctcc acgagccggc cgatgtagtc
      541 gcccttcagc ccgacccagt actcgtccca ccccgtttct ttgtccggcg cgtaccagtg
      601 ccagacctcg ggaaagagca tgaagagcgt gccggggccg acgcgccagc ttcggtcccc
      661 gtcgctgcgg aacgtgcctt tcccccgcgt gatgtagacc agggcgtact cgtcgtagat
      721 acgccccatt tcccattgga acaggtactt gggcgggtgg gctttcggct cgggcgggta
      781 ccggggggcc gtcggcatct tggtgtaccc gacggtggtg cagtagacgc cccactcgat
      841 gtccctcgcc gtggcggcca ggtacttgct gaaggacggg gcggactgcg gcataagccg
      901 cctatagtac caggaatcag ttgacggagt cgatcagctc gttcagcctt cggcgatagc
      961 gggagaactc cggccagtgc acttcggggg gaatgagatg gtcgccgaac ggcacgtagc
     1021 gccccgtggc aagcacctgg cgcaccggct ccaggatctg ctcgatgcgg gccgctccct
     1081 tcacgatctc cgacttcggg ataccgccca tgagctgcag cgtggggaag ctcttgcgca
     1141 ccttgacgat gtccacgccg cagctcgctt ccattggata gaggcccgtc accccggcct
     1201 ccaggaaaaa ggggatgagc gcggtgcact ccccgtccgt atcgacgaag atcgtcttta
     1261 cgccgtgcgc cttgaggaac ccggtgagcc gctggtagta cgggagcatg aactcgcgga
     1321 tggtcggcgg ggccaccatg gagccggtgc ccgccgagat gtcctcccag atgacgaaga
     1381 ggtccacgtc ggtctgcgcc aggacctcct cgtacacggc cagccagatc tcggtgaacg
     1441 tggcgttgat gtcatggatg agcccgggct cgtcggcgta gttgtagaag aggtgctcgt
     1501 agcccatgag gtgcgccagg gtcccgaagt agccgcaggg gtaacctccg aggaccaggg
     1561 ggtagtcgcg gtcgcggtac ctccgcaaca gatccttcca gtgtggcggg aatctaccgg
     1621 cgatgtcctt ggtcgagagg cgctcacgct tgagcgcgtt ccaggaggcc cagtcccgga
     1681 tggggttctc cagggccatc gggatgacgc cggtgacctt ctcgaactgg cgcttcaccc
     1741 cgtcgatatc gacgtagatc atgtgggtgt cggtctcctc gatcacgcgc ggctcgaagc
     1801 gcgggtggaa gaggaggtcg acgttgacca ggacctggcc cgggtccatg gcgcaggccc
     1861 gctggacgtc gtggtccagc gcaaagccct gggtgggcca gtacaggccg ccgcccagga
     1921 cagccatgcc cgccgccagc tcccctttgg gcagcgcgtt ccagcagggg atatacagcg
     1981 aagaggccga cgtcgtggtc ctggtgagcg gccgcggcgg acgatggcgc ggaagcccct
     2041 cccggtacca gttggcgtag ttggcgcccc agtacccgaa ctcccatttg ggagcggcag
     2101 ccttgccgcc ggccatgacc tcgcggaagc gctcgcggac ggtcattgct tcttcagcgc
     2161 gccgctgcgc acgacgtcga tcatcaccgc caggaggagc acgccgccgg aaaccaccat
     2221 ctgccagtag gcgttgatgc tgaggagcgt caggccgttg ttgatggtct ggaggatgag
     2281 gatgcccagg atggtgccgg agagctttcc cttgccgccg ctcaggctga tgccgcccag
     2341 gatgaccgcg gcaatggtgt tcatctccgt tcccgcgccg gactggggaa gcgccgctcc
     2401 cacctgcccc gccagcagga agcccgagat gccggctgcc aggccgctga tgacgaagga
     2461 ggcgaacttc acgcggttga cgttgatgcc cgaaaggtag ctggcgcgct cgttgccgcc
     2521 gaccatgtag accttgcggc cgaaggaggt gaagttcagg aggtagtaga cgatggcaag
     2581 gaccaccacc gcgatgatgg tgacgtaggg gatgatgccg aagagccggc cccggcccag
     2641 gctgaggatc acgggatccg tcaccatcac ggtggcgtcc ttgatgagcc aggcggcgcc
     2701 gcggaagatc tgcatcgtcc cgagagtggt gatgatggcc gcgatcttga acacggatac
     2761 cagggtggcg ttgatgaggc ccatcgacag agccaggagg atgaccagga tcaggacgat
     2821 ccaccacggg gctcccgcgg cggccaccag ggcgcacaga agcgccgtca cggctcccgt
     2881 ggcattctgc gagatgtcca tcccgccgag gatcatggac acggtcagac cggaggcccg
     2941 gatggccagg atcgaggcga acatcaggac gttcatgatg ttctggaggg tgaggaagta
     3001 gggcgacagg ctcgcccaga ccacgcacag gagaaagagc gcgatggcca ggctcagggt
     3061 gctgcccatg gccgcgaccc tgccatacag ggcccggatc cctcccggtg atggtgccgt
     3121 catggtgctc gtgttagcca ttcccgtttc ctttgaccgc caggtgcagg atcgttccct
     3181 ggtcgtattc gcttcggcgg tattcgccga ccaccctccc gttggccagg accagcatgc
     3241 ggtccgtgat gccgatcgcc tccgggagct ccgcggtgac catgatgatg gagatgccgc
     3301 cgcggcacaa ctcctccatg atcttgtaga tctcgacctt tgccccgacg tccacgcccc
     3361 gcgtcgggtc gttcatgatc agcactttcg ggtcggtgag aagccacttg gccacgacga
     3421 ccttctgctg gtttccgccg ctcagcgact ccacgatggc gttggcggag ggcgcgcgga
     3481 tctggagctt ggcgatccac gcctcggcgt acctcttcat cttgcggccg ttcagcttga
     3541 ggccgcgccc cagctcgtcc agcttggtga ccgccacgtt gtctttcagg gaatggatca
     3601 ggaccaggcc ttcctgcttg cgatcgctcg gcacgtacgc gatgcccaac cgcttcgccg
     3661 ccagcggatc gtcgatcctc acctccgctc cgtccaggac gatggtgccg cgcgtcctgg
     3721 cccggatccc caggaggcct tccacggtct ccacgcagcc ggagcccacc agcccgtaga
     3781 gcccgaggat ctcccccttg tgcacggaca gggagatgtc ggtggcgcag ccgcagctca
     3841 ggtgctccac cctcaggacc tcggcgccgg cggaaagctg ctccttcggg tacatctcgg
     3901 cgatcttgcg gccgaccatc atctccacga gcttcccgga ggtggtctcc cccttgctca
     3961 cgacgcccac ccgcttcccg tcccgcatca ccatgatgcg gtccgccagc cgcagcacct
     4021 cttccagctt atgggtgatg tagatcaccg acaccccgcg cttgcgcagg tcctcgatgc
     4081 agccgaagag gcgctccgtc tcgacctcgt tgagcgagga ggtcggttcg tccatgatga
     4141 ggaccttcac gtcccgcgag acggcgcgga ggatctccag catctgtttc tccgccacgc
     4201 tgagggtctt caccagcgtg agcgggttgt agtccaggtt gaagatcgcc agaagctcgc
     4261 gggacgcttt cttcatggcg gcgtagtcca cgcggcgcag gaagcccttc acggggaccc
     4321 gccccatgaa gaagttctcg gcgatcgtca tctcgttgag gtagttgagc tcctggtaga
     4381 tgatccccag ccccagcgcc atgcgctcct tggggttgct ctcgggaggg atggacgtac
     4441 cgtccaggag gatctcgccg gtgtccatga aataggcacc tgccaggatc ttcatcaggg
     4501 tggatttgcc ggcgccgttt tccccgatga cgccgagcac ctcgccgggg cggatctcca
     4561 ggtccacccc atcgagggcg agcacccccg ggaagcgctt cacgacattc ttgacctgga
     4621 gactgtactc gctgcgctct cccaccgtgg aactcaccga tagtcctccg gtccgttgtg
     4681 acgaggggac ccgacgcccg cgcgccaggt ccccgttcgt tccgtgcaaa gcgtccggcg
     4741 ttgccg
//
LOCUS       DASRZS010000682         2979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2293002, whole genome shotgun sequence.
ACCESSION   DASRZS010000682 DASRZS010000000
VERSION     DASRZS010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2293002"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2671)
                     /locus_tag="VFH83_16630"
     CDS             complement(<1..2671)
                     /locus_tag="VFH83_16630"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 78 protein"
                     /protein_id="HET6488056.1"
                     /translation="MKPGSLAATHLLCNGILDPLGVDTGKPLLSWIAESTVGQRGQRQ
                     TAYRIRGGNDPQEMLSEGACLWDTGWVAGGACLQIPWGGPPPRSRQAIWWAVSLRDDA
                     GKAGAWSAPARWEMGLLDPGDWTARWVSADTRVRYAPTPALPAPFLRWEFDLSDEVQQ
                     ARAYVCGLGWHELYLNGNRVGSNQLDPAFTRYDARALYVTHDVTGLLRKGRNAIGGVL
                     GTGWYDQHAADSWSFQHAPWRDPPKLIAQVNVRLASGLELVLGTGPRWRWSHGPILSD
                     AMRNGECYDAQREMPGWAEPGFDDRDWRPVCCAAPPGGRLVSQQMPPIRVTETITPVS
                     VVEPGPGVFVVDMGRVMVGRARIRVRGKPGQRVTLKYAEKTKGNGDIDQSNIDSLTHS
                     GEFQTDVYLCREGGTEWEPRFSTHGFRYVQLKGWPGRPTPADVDGRAVHTDLAGRGEF
                     LSSNPLLNSIQDCCRRSTVYNYHGFPTDCPQRERNGWTGDAHCSAEQALLNFDMDAAY
                     RKWMADIRDCQRPSGQIPAVVPTGGWGFTIGSGPAWDSAAILIPWYLYLYRGDAAILE
                     EHYECMRRYVEFLGAMEIDGIVDFGLGDWCPPEWHPSVVWDPTYRCPVALTDTAYYHL
                     DARILSKVAGLLGKAADEERYETLAARVRRAFREHFLDPATGRVAGDCQASLACALDM
                     GMVGPEERDAVMTRLVQSVEAAGRHIDAGMLGAKSLLRALTDADRVDLAYAIATQTTY
                     PGWGEWIRRGATTLWETWSGDSSLNHHAFSDISAWFYRALAGLRADADEPGFRHFWVR
                     PHPAGDLRWVRARHASPYGPISIAWEIGDGRFDLDLAVPVGARATVQVPADPGSSVRE
                     SGAAAEGRPGVLSVGAPSGGRATVELACGTYRLR"
BASE COUNT          494 a         1020 c          974 g          491 t
ORIGIN      
        1 tgcgaagccg gtaggtgccg caggccaact cgacggttgc cctgcccccc gacggcgctc
       61 ccacggagag cacgcccggc cgcccttccg cggccgcccc ggattcccgc acgctgcttc
      121 ccgggtccgc gggaacctgg accgttgccc gggcgccgac gggcaccgcg aggtccaggt
      181 cgaaccggcc atcgccgatt tcccaggcga tcgagattgg gccatacggg gaggcgtgcc
      241 gagccctgac ccatcgcaga tccccggctg gatgcggtcg aacccagaaa tgtcggaagc
      301 ccggctcgtc ggcatcggcc cgaaggcccg ccagagcccg gtagaaccag gcgctgatat
      361 cgctgaaggc gtgatggttg agcgaggaat cgcccgacca cgtctcccag agggtggttg
      421 cgccgcgccg gatccactcc ccccatccgg ggtacgtggt ctgggtcgcg atggcgtagg
      481 ccaggtccac ccggtccgca tcggtgagcg cgcgcaggag agacttcgcg cccagcatcc
      541 cggcatcgat gtggcggccg gccgcctcca cggattgaac caaccgggtc atcacggcgt
      601 cgcgctcctc ggggcccacc atgcccatat ccagcgcgca ggccagggag gcctggcagt
      661 cgccggcaac ccggccggta gccggatcga ggaagtgttc acggaacgcc cgccgcaccc
      721 gcgctgccag agtctcgtag cgctcctcat ccgcggcctt gccaaggagc ccggcaacct
      781 tggacaggat cctggcgtcc aggtggtagt aagcggtgtc cgtgagagca acggggcagc
      841 gataggttgg atcccacacc acggaaggat gccactccgg cggacaccag tctccgagac
      901 cgaagtcgac gatgccgtca atctccatcg cgccgaggaa ctctacgtag cggcgcatgc
      961 attcgtagtg ctcctccagg atcgcggcgt caccgcggta gaggtacagg taccacggga
     1021 tgaggatggc cgcgctgtcc cacgcgggtc cgctcccgat ggtgaacccc cagcctccgg
     1081 tgggaacgac ggcggggatc tgcccactgg gccgctggca gtcacggatg tccgccatcc
     1141 acttgcggta ggcagcgtcc atgtcgaagt tcagcagggc ctgctctgcc gagcagtgcg
     1201 cgtcaccggt ccagccgttg cgttcccgtt gagggcagtc ggtcgggaat ccgtggtagt
     1261 tgtagaccgt cgaacggcga cagcagtcct ggatcgagtt cagaagggga ttggaggaga
     1321 ggaactctcc ccgaccggcc agatccgtgt ggacggctcg gccgtcgacg tcggctggag
     1381 tcggcctccc cggccacccc ttgagctgga cgtaacggaa cccgtgggtg ctgaaccggg
     1441 gttcccactc cgttccgcct tcgcggcaca ggtagacatc ggtctggaac tcgccgctat
     1501 gggtcaagga gtcgatgttc gactggtcga tgtccccgtt cccctttgtc ttctccgcgt
     1561 acttcagcgt cacccgctga cccggcttcc ccctgacgcg gatgcgcgcg cgcccgacca
     1621 ttacgcggcc catgtcgacc acgaacacgc ccggtcccgg ctcgaccacg gatacgggcg
     1681 tgatggtttc cgtaacgcgt atgggcggca tctgctggga gaccagcctg cctcccggcg
     1741 gcgccgcgca gcacaccggg cgccagtcgc ggtcatcgaa accgggctcc gcccaacccg
     1801 gcatttcgcg ttgcgcgtcg taacactccc cgttgcgcat ggcatcggag aggatcggcc
     1861 catggctcca tctccaacgg ggaccggtcc ccagcacgag ctccaggccg ctggccagcc
     1921 ggacgttgac ctgggcgatc aacttcggcg gatcccgcca gggcgcgtgc tggaagctcc
     1981 aggaatccgc ggcgtgctgg tcgtaccagc cggtgcccag gacgccgcca atggcgttgc
     2041 ggcccttcct caggaggccg gtgacatcgt gggtaacata gagggctcgc gcgtcgtagc
     2101 gcgtgaacgc gggatcgagc tggttgctcc ccactcgatt gccgttgagg tacagctcgt
     2161 gccagcccag accgcagacg tacgctcggg cctgctggac ctcatccgac aggtcgaact
     2221 cccagcgcag gaacggagca ggaagcgccg gggtcggtgc gtatcgcacc cgtgtgtccg
     2281 cggagaccca tcgagccgtc cagtcccctg gatccaggag ccccatttcc caccgcgcgg
     2341 gcgcgctcca tgcaccggct ttcccggcgt cgtctcggag gctgaccgcc caccagatcg
     2401 cctgcctgga ccgtggcggc ggacctcccc acgggatctg gagacacgcg ccgcccgcca
     2461 cccacccggt atcccacagg caggcgcctt cgctcagcat ctcctgcgga tcgttccctc
     2521 cgcggatccg ataggcggtt tgtcgctgcc ctcgctgacc cacggtggat tcggcgatcc
     2581 agctcaagag gggcttcccc gtgtccacac ccaggggatc caggatgccg ttgcagagca
     2641 gatgggtcgc ggcaaggctt ccgggcttca tgggatggga tggtacagcc cgtcacgccg
     2701 gaccgtcaac aaatgaggat ctcggcagcg cattgcgggc gcaattcaac cagaacctga
     2761 gagatcttgt ccgaacgttg ggagattctg cttgacttcg caggtatccc atgtttgcat
     2821 aaaggcaatt ctggcagaat cctgcctaag gaggtcgtat gtctcgaaag ctcatggttc
     2881 tgctggcgct gctgctggtc gggctcttcc tgacgaccat gcaggcagtg gcggcaggga
     2941 aggcagaggc gggcaagatc gataccctca ccttctggt
//
LOCUS       DASRZS010000683         5031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_1923865, whole genome shotgun sequence.
ACCESSION   DASRZS010000683 DASRZS010000000
VERSION     DASRZS010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923865"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="VFH83_16635"
     CDS             complement(<1..1083)
                     /locus_tag="VFH83_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6488057.1"
                     /translation="MRILRQPLHSGRISPMLYGGFIELLDDLVPGMWAEMLNDRSFEG
                     VLPPSSWCYYNGVPNACDRPWESSDSWRLDSTDPFDGERSARLLAGPAAAASLCQEGL
                     AVRKGMTYRFEGWFRAQGRNLEPTVRLKTRHPDGSWIELASHALSVPASAWAKQSFEL
                     VSAGTTDRAVFELSAFGSSTLWADKLSLMPKDNQNGWRRDVVEAVRAARPGLIRWGGS
                     VVDPGGYRWKIGIGDRDRRSPFANRPWGRMDPNDVGIDEFLQFCELVDARPLVCVSFS
                     DGPQSARDLVSYCNGGADTPWGGRRTANGHAEPYGVRLWQIGNELGDADYVRGCTAIC
                     RAIKETDPQSLVLSSYPSPALLAEAGR"
     gene            1243..3747
                     /locus_tag="VFH83_16640"
     CDS             1243..3747
                     /locus_tag="VFH83_16640"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HET6488058.1"
                     /translation="MAFATLLLVSVIANRSEASAPDGHSLRPAVAGLIDLRSWDPGGP
                     TVARLNGEWTFLWDVLADPAATAAAMRAGGVPGDATIFVPQSWRYYTIEGSPTPDMGR
                     ATYALRILLPNRDLPLFLHVSVISSAARIYANGTLVASRGRVGSSYEEELPDWSPEVL
                     LLPAVGPELDLVIHVSNFHHDRGGIIRALELAGPAAAPYLREPSVAFELFLVGTLLVM
                     GAYYTALFFLRRRDTAPLYFGVFCLIICLRTLVTGDTIYVLRILPGLSWELLQKAASL
                     SFVAAVPVFCAFVRSLFPDVYPRLLLRIQSGIAALLGAVVVATQARLYTYLDVPYQVF
                     TVLCVTFIAVAVLRASRRRSPGALVFLVGFGVLVATTVNDILFANDVIHTADLVGLGA
                     TFFILSEAFFLSRRMSGAFVLVERQAEELLGMNRAYAREIETRQATEARLRESQETAR
                     ALLDVPSADAVLIDRDGIILSHNESFRVNQARHTSGSLTGACVWDLFPPDVADRRRTL
                     VRSAVESGRLLRYEDESGGRWRDIVIHPVESPGGGVSRVAVLAVDITDRKRMEREAAE
                     HQRRLVEADKLSSLGVLVAGVAHEVNNPNHAILLNARFLDRAAADIVSALDESPAAQP
                     DTRFGGVEYPELRRRLPEALTLIRRGAERIDSIVSQLKAYARDDTAQPAENVDANEAV
                     RSALALASLDVRRATDLLRVDLAPGLPPIRGHRQRLEQVIINLVQNAANALRSRDEAI
                     EVSTRNDAARGLVSIHVRDEGMGMREEDVARIRDPFFTTRRSSGGTGLGLSVSASIVE
                     QHGGSLEFHSVLGQGTEAIVRLPSAPSDAAPRTADA"
     gene            3747..>5031
                     /locus_tag="VFH83_16645"
     CDS             3747..>5031
                     /locus_tag="VFH83_16645"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6488059.1"
                     /translation="MLLHPPHPILMVDDEEAILMTQETVLHAAGLTNTLRCGDATRVL
                     PMVRAQEIEIVLLDLSMPRLSGQDILRSLQQEFPEIPVIIVTGTNDIESAVTAMQAGA
                     FDYMVKAVEESRLLSGVRRAVEMRALRREYTDLRSHLLDDRLRCPEAFAALVTRSRCM
                     HSLFLYAESVARTGETVLVRGETGTGKELIAAALHAASGRTGDLVTVNTAGLDEAMLS
                     DVLFGHRKGAYTGAEESRDGLIKAAAGGTLFLDEIGDLSPASQVKLLRVLEKGEYYPL
                     GSDLARTSACRFVVATGADLEDLMRRGTFRRDLYFRLSTHELRVPALRERKEDLPLLF
                     SHFLDLACRELGKERVAVPPQIMEYLQAYDFPGNVRELRSMVYNAVSTHTSRMLSLSA
                     FPRLKPLEPSRSVSPPALGFPDRLPTIRESIDLLVE"
BASE COUNT          759 a         1737 c         1687 g          848 t
ORIGIN      
        1 ccttccggcc tccgccagga gcgcgggtga cggatacgag gacagcacga gggactgggg
       61 gtccgtctcc ttgatggcgc ggcagatggc ggtgcatccc ctcacgtagt ccgcgtcccc
      121 cagctcgttg ccgatctgcc agaggcgcac accatacggt tcggcgtgcc cgttggctgt
      181 ccgcctcccg ccccacgggg tgtcggcgcc tccgttgcag tacgaaacga gatcccgggc
      241 gctctgcggc ccgtcggaga agctcacgca caccaggggc cgggcgtcga ccagctcgca
      301 gaactgcagg aactcgtcga tgcccacgtc attcggatcc atccggcccc acggacggtt
      361 ggcgaacggc gagcggcggt cgcggtcccc gatcccgatc ttccagcgat agccgcccgg
      421 gtccacgacc gacccgcccc agcggatgag gcccggtcgg gcagcccgga ccgcctccac
      481 aacgtcccga cgccagccgt tctggttgtc cttcggcatg agggaaagct tgtccgccca
      541 gagcgtgctc gagccgaatg cggacagctc gaaaacggcc cgatcggtcg tacccgcgct
      601 gaccagctcg aagctctgct tcgcccacgc tgaggcaggg acggatagcg cgtgggaggc
      661 aagctcgatc cagcttccgt ccggatgccg tgtcttgagg cgcaccgtcg gctccaggtt
      721 ccgaccctga gcgcgaaacc agccctcgaa ccggtaggtc atccctttgc gcaccgcgag
      781 cccttcctgg catagggacg ccgcagcggc cgggccggcc aacaggcgcg cggagcgctc
      841 tccatcgaag ggatccgtgg agtccagccg ccaggagtcc gatgactccc aaggacggtc
      901 gcaagcgttg ggaacgccgt tgtagtagca ccacgaggag ggcggcagga ctccctcgaa
      961 gctccgatcg ttcagcatct ccgcccacat cccggggacc aggtcgtcca ggagctcgat
     1021 gaagccgccg tacagcatgg ggctgatccg gccggaatgc aggggctggc gcaggattct
     1081 catcacccgc cagtataacg ggtttgtcgg tctttgcccc agcccatcgg gttttcgcta
     1141 tattagggga gcatttcagg agcgcaggca ggaggttcga cgatgcggcg aagcgcttgc
     1201 cggcggccca ggatctgggc ggtatgccac ccgtcgtcgc gggtggcttt cgcaacgctt
     1261 ctactggtct ccgtcatcgc caacagatcc gaagcatccg cgcccgacgg gcattccctc
     1321 cgtcccgccg tagccggcct gatcgatctt cgctcctggg accccggggg gccaacggtt
     1381 gcccgactga acggggagtg gacattcctc tgggacgtcc tggcggatcc ggccgcgacg
     1441 gccgcggcca tgcgggcggg cggcgtaccg ggggatgcga cgatcttcgt cccgcagagc
     1501 tggcggtact acacgatcga gggttccccg accccggaca tgggacgggc aacctatgcg
     1561 cttagaatcc tgcttcccaa ccgcgacctg ccgctcttcc tccacgtgtc ggtgatctcc
     1621 tccgccgccc ggatctacgc caacgggacc ctggtggcga gccggggccg tgtcggatcg
     1681 agctacgaag aggagcttcc cgactggagt cctgaggtgc tgcttctgcc cgcggtcggt
     1741 cccgagctcg acctcgtcat ccacgtctcc aacttccatc acgatcgagg cggcatcatc
     1801 cgggccctgg agctggcggg tcccgcggcg gccccctatc ttcgggagcc gtcggtcgcg
     1861 ttcgagctct ttctggtcgg cacgctcctg gtcatgggcg cctactatac cgcccttttc
     1921 ttcctcagaa gacgcgacac ggccccgctt tacttcgggg tgttctgcct catcatctgc
     1981 ctccgcaccc tggtgacggg agacaccatc tatgtcctac gcatccttcc cggcctctcc
     2041 tgggagctcc tgcagaaagc cgcctccctc tccttcgtag cagccgtgcc cgtgttctgc
     2101 gccttcgtcc ggtccctgtt tcccgacgtc tacccgcggt tactcctgcg gatccaatcc
     2161 ggcatcgcgg ccctccttgg cgccgtggtg gtggcgacgc aggcccgcct ctacacctat
     2221 ctggacgtgc cctaccaggt cttcacggtc ctctgcgtga cgttcatcgc ggtggcggtc
     2281 ctgcgggcgt ctcggcgccg aagtccgggg gctctcgtgt tcctggtggg gttcggggtg
     2341 ctcgtcgcca cgacggtgaa cgacatcctg ttcgccaacg acgtgatcca caccgcggat
     2401 ctggtgggcc tgggcgccac gttcttcatc ctgagtgagg ccttttttct ctcccgtcgg
     2461 atgtccggag cctttgtgct ggtggagcgt caggcggagg agcttctggg gatgaaccgt
     2521 gcctacgccc gggagatcga gacccggcag gccaccgaag cgcggttgcg ggagagccag
     2581 gagaccgccc gcgccctgct ggacgtccct tccgcggacg cggtcctgat cgaccgggac
     2641 ggcatcatcc tctcccacaa cgagtccttc cgggtcaacc aggcgcggca cacctccggg
     2701 tcgctgaccg gcgcctgtgt atgggatctg ttcccgccgg acgtggcgga ccgccgtcgg
     2761 accctggtgc ggagcgccgt cgagagcgga cggctcctgc gttatgagga cgagagcggc
     2821 ggccgatggc gcgacatcgt gatccatccg gtcgagtccc ccgggggcgg cgtgtcccgg
     2881 gttgccgtgc tggccgtgga catcaccgac cgcaagcgca tggaacggga ggcggccgag
     2941 catcagcggc ggctggtgga ggcggacaag ctctccagcc tgggggtcct ggttgccgga
     3001 gtggcgcacg aggtgaacaa cccgaaccat gccatccttc tgaacgcacg cttcctggac
     3061 cgggccgcgg cggacatcgt ctcggcgctg gacgagagcc ccgcggccca gccggacacc
     3121 cgcttcggcg gcgtggagta cccggagctt cggcggcggc tgccggaggc gctcacgctg
     3181 atccgtcgcg gggccgagcg gatcgactcc atcgtatccc agctcaaggc gtacgcgcgg
     3241 gacgacaccg cgcagccggc cgagaacgtc gacgcgaatg aggcggtgcg gtcggccctt
     3301 gcgctggcgt ccctggacgt ccgccgcgcc acggatctgc tgcgggtgga ccttgcgccc
     3361 gggctgccgc ccatccgggg tcatcgccag agactggagc aggtgatcat caacctcgtg
     3421 cagaacgccg ccaacgcgct gcgctcccgc gacgaggcca tcgaggtctc gacgcggaac
     3481 gacgcggccc gcggcctcgt ttccatccac gtacgggacg agggcatggg gatgcgggag
     3541 gaggacgtgg cgcgcattcg agatccgttt ttcacgacgc gccggtcttc cggaggcacg
     3601 gggctggggc tgtccgtgag cgccagcatc gtggagcagc atgggggctc gctggagttc
     3661 cactcggtcc tggggcaggg aacggaggcc atcgtccgac ttccttccgc gccgagcgac
     3721 gcagccccgc gaacggcaga cgcctgatgc tgctgcatcc cccgcacccc attctcatgg
     3781 tggacgacga ggaagccatc ctgatgacgc aggagacggt cctgcacgcg gccgggctca
     3841 cgaacaccct ccgatgcggc gatgccaccc gggtcctccc catggttcgc gctcaggaga
     3901 tcgagatcgt ccttctggac ctgtccatgc ccaggctgag cggccaggac atcctgcgct
     3961 cgctccagca ggagtttccg gagatcccgg tcatcatcgt caccggtacg aacgacatcg
     4021 agagcgccgt aacggccatg caggcggggg ccttcgacta catggtgaaa gcggtggagg
     4081 agagccggct tctgagcggc gtgcggcgag cggtggagat gcgagccctc cgtcgggagt
     4141 acacggacct gcggtcgcac ctcctggacg accggctgcg ctgcccggaa gcgttcgccg
     4201 ccctggtcac gcggagccgg tgcatgcact ccctgttcct ctacgcggag agcgtggccc
     4261 ggaccgggga gaccgtcctg gtgcggggag agacgggaac ggggaaggag ctgattgccg
     4321 cggcgctcca cgccgccagt gggcgcaccg gggacctggt gaccgtaaac acggccgggc
     4381 tcgacgaggc gatgctgtcc gatgtcctct tcggccaccg caagggggcg tataccggcg
     4441 ccgaggaaag ccgggacggg ctgatcaaag ccgctgctgg cgggaccctc ttcctggacg
     4501 agatcggtga cctctcgccg gccagccagg tcaagctcct tcgagtgctg gagaagggcg
     4561 agtactaccc gttgggctcg gatctggccc gtacctcggc gtgccgcttc gtggtggcaa
     4621 cgggcgccga cctggaggac ctcatgcgtc gggggacctt ccggcgggac ctctacttcc
     4681 ggctctcgac ccacgagctg cgcgtccccg ccctccggga gcgcaaggag gatctgccgc
     4741 tcctcttcag tcacttcctg gacctggcgt gccgagagct ggggaaggag cgggtggcgg
     4801 tgccccccca gatcatggag tacctgcagg cctacgactt tcccggaaac gtgcgagagc
     4861 tgcggtccat ggtctacaac gccgtgagca cccacacgtc ccggatgctg tccctctccg
     4921 cctttccacg actgaagccg ctggagccgt cgcgatccgt ttcaccgccc gctctcgggt
     4981 ttccggaccg gctccccacc atccgggaga gcatcgacct cctcgtggag g
//
LOCUS       DASRZS010000684         4099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2294495, whole genome shotgun sequence.
ACCESSION   DASRZS010000684 DASRZS010000000
VERSION     DASRZS010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2294495"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..3031
                     /gene="nifJ"
                     /locus_tag="VFH83_16650"
                     /pseudo
     CDS             1..3031
                     /gene="nifJ"
                     /locus_tag="VFH83_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253227.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
     gene            3028..3519
                     /locus_tag="VFH83_16655"
     CDS             3028..3519
                     /locus_tag="VFH83_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010315578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET6488060.1"
                     /translation="MSHPHTYQAALTWEGNLGQGTSTYQAYSRLWRVRFTGKPDLLGS
                     ADPTYRGETDKHNPEDLLVASLSSCHMLTWLALCARNKITVVSYVDNATGVMETTPDG
                     GGRFVSVTLNPKAEITDGAQKELAERLHQKAHELCFIAASVNFPVHHAAEVVAPERRP
                     QQP"
     gene            complement(3455..>4099)
                     /locus_tag="VFH83_16660"
     CDS             complement(3455..>4099)
                     /locus_tag="VFH83_16660"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HET6488061.1"
                     /translation="ELLLRLPALRSLNLVSNPLKVLPSGLGDMNGLRELYLAGLHLRE
                     VPGWIARLRGLRRLALYDNELEALPEWVGDLRSLRELTVGSNRLHALPSSIGALTRLV
                     ELSAYGNRLEGLPESVGRLRRLRELALQGNRITELPPGLAAMRALRYVILSDNRLDSL
                     PPGMRRLRGLRALHLRGNPLGALSSELEGWLSGLKAAGVDVRGQLPPRRDGPGS"
BASE COUNT          802 a         1383 c         1301 g          613 t
ORIGIN      
        1 ccagtagatc gaggagctgt ccgtcgacga cctgcggacc atgctgcccg acgagctgat
       61 cgcggcccac cgcgcccggg cgctcaatcc cgagagcccc accatcaagg gcacctccca
      121 gaaccccgac gtgtactttg ccggccgcga ggcggtgaac aggtactacc tggcggtccc
      181 ggcaatggtg cagaaggcca tggacaagtt cgccggaatc accggccgcc agtaccggct
      241 cttcgactac gccggcgccc ccgacgccga ccgcatcatt atcatgatgg gcaacggcgc
      301 ggagaccgcg gaggagtgcg ccgagtacct caacgcgcac ggggagaagg tgggcgtcct
      361 gaaagtgcgc ctgttccgcc ccttcagcct ggagcacttt gccaaggcca tcccgtccac
      421 cgtcaagtcc atcgccgtcc tggatcggtg caaggagccc ggctcgttgg gcgatcccct
      481 gtacgaagac atccgcaccg cggtcgggga gggcatggcg aacggtcttc tgtccttcaa
      541 gcagacgccg accatcgtcg gcggccgcta cggactgggg tcctacgagt tcacgggagc
      601 catggccaag tccgtgttcg acaacctcaa ggctgccacg cccaagaacc acttcaccgt
      661 cgggatctac gacgacgtga cccacaccag cctgccgttc gacgagtcct tctcctcgga
      721 gccggcgggg gtccacagcg ccctgttcta tggactaggc gcggacggga ccatcggcgc
      781 caacaagaac tccatcaaga tcatcgggaa agcgacggac aactacgccc agggctactt
      841 cgtcctggac tccaagaagt ccggctctat cacgatctcc cacctgcgct tcggcaagcg
      901 gcccatcaag agcacgtacc tgatctccaa ggccagcttc ctggcctgcc acaagttctc
      961 cttcctggac aagtacgaca tgctctccaa ggcgagcgac ggagccgtgt tcctcctgaa
     1021 ctccccctac ggcaagaccg agatctggga gcacgttccc gtcgaggtgc agaagcagat
     1081 catcgacaag aagctcaggt tctacgtgat cgatgccacg aagatcgcca acgaggtggg
     1141 tctgcgggga cgcatcaacg tggccatgca gacggcattc ttcgtcatct ccggggtgct
     1201 gccggaggcc aaggccctgg agctgatcgg ggccgccatc gaggacacct acggcaacaa
     1261 gggcaaggac gtcgtggcca tgaacatgag ggcggtggag gcggcccgga cccggatcga
     1321 gaaggtggag tatccctcca cgcctacggg ctccgagcac atgaagccgc cggttcccgc
     1381 ggaggcgccc gagttcgtgc gcacggtgac cgcagagatc attgccggac gcggagagta
     1441 cctgccggtg tcgaagctcc cgtacgacgg cacctggccg acggggacca cgaagtggga
     1501 gaagcgcaat atcgccgagg agatcccggt gtgggactcg gagacctgca tccagtgcgg
     1561 cgagtgctcc atggtctgcc cgcacgcggt catccggatg aaggtctacg acccggcgct
     1621 cctcaaggac gccccggcct ccttccagtc cgtcgacgcc aagggcaagg agttcgaagg
     1681 ccagaagttc accatccagg tctcctccct ggactgcacc ggctgcggcg tgtgcgtgag
     1741 cgcgtgcccg gcctacaaga aggtgaacgg agagaagacg gaccgcaagg ccatcaacat
     1801 ggcgccgcag ctccccatcc gggaccagga ggcgaggaac ttcgagttct tcctctccct
     1861 gcccgaggtg gatcccaagt tcatcaagcg gaacaccatc aaggggtccc agctcctgat
     1921 gccgctcttc gagttctccg gcgcctgcgc cggctgcggt gagaccccgt acgtcaagct
     1981 catgagccag ctctatgggg atcgggcgat catcgccaac gccacgggct gcacgtccat
     2041 ctacggcggg aacctgccca ccacccctta cacgacgcgg gcggacggac gcggaccctc
     2101 gtggtccaac tccctgttcg aggacgcggc cgagttcggc tacggcatgc ggctaacggc
     2161 ggacaagctc ttcgagtacg ccaacgagct catcgacacc atgctttccg ggaacggcac
     2221 cgcggcgccg cgggagcttc tcacccagat ccgcacggcg gaccagtcca cgcaggccgg
     2281 catcgaggct cagcggagcc gcgtcgccga tctgaaaaag gccctggcca aagggaagtc
     2341 ggaggcggaa cggcgcctgc tgacctccgc ggactacctg atccgcaagt ccgtctggat
     2401 cctgggcgga gacgggtggg cgtacgacat cggctacggc ggtctggatc acgtgatcgc
     2461 ctccggtcgg aaggtgaaca tcctggtcct ggataccgag gtctactcga ataccggcgg
     2521 ccagatgtcc aagtccacgc cggtgggcgc catcgccaag ttcgccgccg gcggcaagcc
     2581 gaccgggaag aaggacctgg gcctcatggc catcgcgtac ggcaacgtct atgtggcccg
     2641 cgtggccttc ggctccaacc ggctgcagac catccgcgcc ttcaacgagg cggacgagta
     2701 tccgggtccg tccctcatca tcgcttacag ccactgcatc aaccacggcc tggatctccg
     2761 caaggggatc agccagcaga agatggcggt ggactccggc gcctggacgc tgttccgcta
     2821 caaccccacg ttgcgcgcgc agggcaagaa ccccatgacc ctggactcca aagagccctc
     2881 ggcggacatc ggcgactaca tgtacaacga gatccgcttc cgggccctca agcagatcgg
     2941 gccggagcgc gcctcagagc tcctggaggt agcccgcaag gacgccaagg agcggtacgc
     3001 gtactacaag tacctggcgg acaggacgtg agccacccgc acacgtacca ggcggccctg
     3061 acctgggagg ggaacctcgg tcagggcacc tccacctacc aggcctacag ccgcctctgg
     3121 agagtgcggt tcaccggcaa gccggacctg ctggggtcgg cggatccgac gtatcgcggg
     3181 gaaacggaca agcacaaccc cgaggacctg ctcgtggcgt ccctcagctc ctgccacatg
     3241 cttacgtggt tggcgttgtg cgctcggaac aagatcaccg tggtgagcta cgtggacaac
     3301 gcgaccgggg tgatggagac gacgccggac ggaggcggcc ggttcgtctc ggtgaccctg
     3361 aatccaaagg cggagatcac cgatggggcc cagaaggagc tggccgagcg cctgcaccag
     3421 aaggcgcacg agctgtgctt tatcgccgcc tccgtcaact tcccggtcca tcacgccgcg
     3481 gaggtagttg cccccgaacg tcgaccccag cagccttgag ccccgagagc cagccctcaa
     3541 gctcggagct cagggcgccc aacgggttgc ctcgcaggtg aagcgcccga agtcctctca
     3601 ggcggcgcat gcccggaggc aggctgtcca gtcgattgtc cgaaaggatc acgtagcgta
     3661 gggccctcat ggccgcaagg ccaggcggta gctcggtgat ccggttcccc tgaagcgcga
     3721 gctcccgcag ccgcctcaga cgcccaaccg attccggaag gccctccagg cgattcccgt
     3781 acgccgagag ctccacgagg cgcgtcaacg ctcctatgct cgacggaagc gcgtgaaggc
     3841 gattggagcc caccgtaagc tcgcgtaggg aacgcaggtc tcccacccac tcgggcaggg
     3901 cctccagctc attgtcgtac aacgccagtc ggcgaaggcc gcggagacgg gctatccacc
     3961 ccgggacctc tctcaggtgg agtccggcaa gatagagctc gcggaggccg ttcatgtcgc
     4021 cgaggccgct ggggagcacc ttcaacggat tcgacaccag gttgagcgag cgcagcgcgg
     4081 gcaggcggag gaggagctc
//
LOCUS       DASRZS010000685        10856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_380637, whole genome shotgun sequence.
ACCESSION   DASRZS010000685 DASRZS010000000
VERSION     DASRZS010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10856
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380637"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFH83_16665"
     CDS             complement(<1..579)
                     /locus_tag="VFH83_16665"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6488062.1"
                     /translation="MAARSQAPRPWVIAAEGLARVRERHPLVTFLIGRVVTMGIMMFF
                     LGLGVFGLMSLAPGNIIDDYARNQVLGGGGFRSNDNALTAEQIALAKHQLWLDRPFAV
                     QYFHWLHQVFIEHDLGRSLISRAPVLFLVSTRMINSIVLNLISLVFLTILSFGLGIYF
                     SSKAGTRLDPAVAFVSLFLNACPGILLLLLLQV"
     gene            complement(579..2408)
                     /locus_tag="VFH83_16670"
     CDS             complement(579..2408)
                     /locus_tag="VFH83_16670"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6488063.1"
                     /translation="MRTSAGAPIAVVAVTALAALAACGPRVAQLTPDQVAFQKKVEQN
                     ALLAKTVNRPGSTAPYPKGAHGGTWQSDLNDDPKSFNMLHSQDTETDAVVGVLADYLL
                     DYDAYKRQWKPNVASYEVKVNDQAQTMDVTFTLRDDLYWTTLADPSKKVKVTSDDVVY
                     WYNDIVGDQALQQSGYSGQFLQMPDGGKKHIDMEKIDDRRFVMHYPRIVAMPELSSNM
                     NFGPRYIFEPVKKAKGVEGLLNLWSIDTDPKTIPSMGQYYIQSYRPGVGVTLVRNPNY
                     WKKDDYGQQLPYIDTIEMKIVPNRDTEKLKFLANELDSYSLRPEDLSDMVAKDPRNYS
                     VYYAGPSLGAAFISWNQNPKNLAPKYLKWFSNTTFRQAMSCFLNRDRIVTEVYRGLAD
                     PDLSFFAKPNPFYDPAIAEQYTYNPARGKELLAEIGIKPNANGLMQDSEGNVVTYDLE
                     VTVENNVAVDIANIFADELKKVGITLNVKPLQFQKIVQTLMQTYDWQCVMISVGSNYF
                     PIQGDNVWLSSGNLHLWNPLEAKPATAWESQIDDLYWKGYSTRDNAEAKKIWDQYQRI
                     ILDQLPVMYLTYPDVFSAYRDRWANLRVDALSAPDMNYVYLKP"
     gene            2582..3931
                     /locus_tag="VFH83_16675"
     CDS             2582..3931
                     /locus_tag="VFH83_16675"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HET6488064.1"
                     /translation="MSPVTFPKGFLWGASTAAYQIEGYPLADGASPSIWHEFVQRTGA
                     IRDGTTGDIACDHYHRYAEDVGAMSALGLNAYRFSIAWPRILPEPGRVNPKGLDFYSR
                     LVDCLLEAGITPYATLFHWDVPTWLEEEGGLVERTSVDHLVRYAETVVKSLGDRVRHW
                     ITINEPTIFALFGYITGEYPPGRTRDLRAAFRAIHFLLLAHARQVRVLRALAPGAEVG
                     LAHHFVWIDPRDPRRRRDVRAATEMDDFANHAVLESLVRGTYPPTITSRVGRFLPKGF
                     ERDLQEMGIPGDFIGMNYYTRQQYRHALLMPYLHAREYTDPSAPRSAMWEIHPDGMYR
                     WLVRLRDRYGNPRCIVTESGFPLPDVQGRDPLDDPERISYLTDHIARIGKAIQEGARC
                     EGYFHWSLLDNFEWSYGTIMRFGLLRTDFDTQKRSWKRSAYWYRDLVRANALPADGA"
     gene            complement(3961..4959)
                     /locus_tag="VFH83_16680"
     CDS             complement(3961..4959)
                     /locus_tag="VFH83_16680"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6488065.1"
                     /translation="MANLTIRDVAKEANVSVATVSRVLNGRVGYTEETKARVLEVIDR
                     LEFRRNALATGLVSRRTSTIGVLLPRVSDRCSTLLLHGIEDGAHELSHSVIVCNTDDD
                     DSRTLHDLRVLGEHQADGVIFAGSNLTDECGRELERMGIPAVLVSTSSTRYQLPYVRV
                     DDTAAAFHAARYLVENGHRIIGMIAGPQKDPISGVTRVEGFLRALREEGLDAGGERVA
                     YGDLRFKSGLDAMDRLMAQVRGITAVFAASDEMAVGAIAWCGAHQIRVPEQMSVIGYN
                     DTPDAEMATPPLTTVRQPIYEMGKRAVKILVDRRHRQESLVMPFNIVERASVRRCA"
     gene            5245..8400
                     /locus_tag="VFH83_16685"
     CDS             5245..8400
                     /locus_tag="VFH83_16685"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HET6488066.1"
                     /translation="MAVAVTAFLFMLSGCDLLNKILNNPPVADAGKDQTVQIDTQVTL
                     DGHNSRDPDGNQLTYSWSITSQPPTSTVNNSKVSGASSQVAYFTPDVEGLYIAELKVS
                     DGQADSTDTVSITATASGGGGGGGGGGSAPSAPSINVAAGDAYVTVTWAGVSGATSYN
                     LYYKLGGSVTIATGTKLSNVTSPKDVTGLANGSQYAFIVTAVNSNGESGASNTATATP
                     VLGMPSAPTGLGVTTSGNQLQVTWGAVAGAINYFVYKDTNPTGSYASIAYGGGNTSFT
                     DGPLTSGTTFYYEVAVQTNIGTSGKSAYVAGTTVPAAPSIQSVAAGESQVAITWGNVA
                     GANSYNLYWAAGSTATTGSNKISGVGSPYTVTGLSDGTQYAFIVTAVNAGGESLPSAA
                     QTATPQMGIPTSPSSLLITGASGTTLSLSWSAVGGATGYQLFRDTSSGGSFSTQRYSG
                     SALSFTDSNLTQGTTYYYKVRASNGAGTSPLSNWQSGTTIPSSPTASAVPGDGQVTVS
                     WAAVPGATSYNVYYAAGSSVTIGGAGVTTVLNANSPCLVSSLTDGIQYAFIVTAVNGG
                     GESAASSPATATPQPGLPSTPQNLWVPSATATSLTVQWDAVSGTGVSYVVYRATDQAG
                     PYGFQAYSGTSTSFTSNSLASGTTYYFEVKAHNASGFSALSTPSSGTTLPAAPSISVT
                     AGDQQVTVSWASVAGADSYYLYYKVGASVTTATGSMVAGVTSPYIMPSLANGTQYAFI
                     VTASDSSGEGAASNVATGTPAPGIPQAPSGLTVFSPTGTSLSVAWNYVSGATSYQVYR
                     DTAQFGTYSFLAYNGNSTGFTDTGLSSATTYYYEVRATNASGSSSLSAPVSGTTSLVA
                     PNTPVGLSIAGVTANTVTVSWVVPSGATSVQLYRDTSQLGSFSLLVYSGSASSYQDTG
                     LSGGTDYYYKVQATNDAGSSSKSAWIKGTTTTIAPSTPTGLLVGNPTSGSLDVSWNAS
                     PGATWYSVTRSTDATFVVSNNVYNGGATGFTDTGVASSTTYYYKVSAGNSAGSSLQSA
                     AASGTTQALTSGEGGYGYGIR"
     gene            8462..10357
                     /locus_tag="VFH83_16690"
     CDS             8462..10357
                     /locus_tag="VFH83_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6488067.1"
                     /translation="MKRSIYLLSIVALAAFILGLGACNLFDSPLTPVPSGTGKGGITI
                     KLQGVSPAMQQLVTQVVKRNQPVSGKALSPKMYLGAQRVEFELWDNPPTSVQDAWTAS
                     LDQVNPGNSFIIGTRSHAPGDYQLRAYVYNDPNDITPTVSGIEPFHITADQDAASHQV
                     VVTCFPVNPVVVPESTPSLSYTMAKPWIVNFNNGVISQIGSEQWFQATAQSDHTTFTV
                     SPDAGSGALPVIMVWDSTGQIVGQGMPTAPGVATTAGNIPTTIGGTYYFAAADFSDPT
                     TPVRSFRVSYQPCSITEDLAVSVTPDTTGSPAYTGYSIQTFHAQWYYFSGLQGHIYTV
                     NWDDVGTGSNLYTGDVQVSAYRQDQSTPYFQAIDGGYVGSSYYSNGNYWTYSIDETQT
                     FTPQTSELVYIKVEERNAGATAGTYGLRIEDLGTNHPPWAYAWTNSSSVGTGSPVSIG
                     VNAGDPDMGDTLTYSWVVASGPDYNGLSNANQPYATFTPDAAGTYQLTVTVTDSGTPQ
                     HSASSSCYVTAVYNAPPVIDWAYPSTAEITLSGSSVIYLYSGAHDPDGPSVTYQWQLT
                     SVPSGSWASIVGANQANATLAPDVPGAYELQLTVTDTLGGKTPSATITITVDANPGAG
                     GIGVGIN"
     gene            10381..>10856
                     /locus_tag="VFH83_16695"
     CDS             10381..>10856
                     /locus_tag="VFH83_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6488068.1"
                     /translation="MKIKRSLYILGAAVGASILVLASCNPMLANTLAPAAAPASAGHS
                     SPTAGKGGVGISVQAISPVVNNVFQKQLLAQGSSKGLSSKAYLGAGRVEFTVYDVTDT
                     YVIDGWTVYPSQNSPSQGGYSLGAWYGAPYNGYVLHAKVYNQPGDGVPAVQGESYF"
BASE COUNT         2018 a         3545 c         3343 g         1950 t
ORIGIN      
        1 cacctgcagc agcagcagca gcaggatccc gggacacgca ttcaggaaga gactcacgaa
       61 ggcgaccgcg gggtcgagcc gggtccccgc cttggaagag aagtagatcc cgagaccgaa
      121 agacagaatg gtcaggaaca ccagggagat caggttcagg acaatggagt tgatcatccg
      181 ggtcgacacc aggaacagga ccggggcccg ggagatgagg gaccgaccca gatcgtgctc
      241 gatgaagacc tgatggagcc agtggaagta ctggaccgcg aacggcctgt ccagccagag
      301 ctggtgtttg gccagcgcga tctgctccgc ggtcagggcg ttgtcattgg agcggaaccc
      361 gccgccgccc aggacctggt tcctggcgta gtcatcgatg atgtttcccg gcgccagaga
      421 catcagtccg aaaacgccca ggcccaggaa gaacatcatg atccccatcg tcacgacccg
      481 cccgatgagg aacgtgacca gaggatggcg ctcgcggact ctcgccaggc cttccgcggc
      541 gatcacccac ggccgcggag cctgcgagcg cgccgccatc acggcttcag gtacacgtag
      601 ttcatgtccg gggccgacag ggcatccacc cggaggttgg cccatcggtc ccggtacgcg
      661 gagaagacgt ccgggtaggt caggtacatg accggcagct ggtccaggat gatccgttgg
      721 tactggtccc agatcttctt ggcttccgcg ttgtctcgag tggaataccc cttccagtac
      781 agatcgtcga tctgggactc ccatgcggtg gcgggcttgg cttccagggg gttccacagg
      841 tggaggttgc ccgaggagag ccacacgttg tcgccctgga tggggaagta gttgcttccc
      901 acggagatca tcacgcactg ccagtcgtag gtctgcatca gcgtctggac gatcttctga
      961 aactgcagcg gcttgacgtt gagagtgatc ccgaccttct tgagctcgtc ggcgaagatg
     1021 ttggcgatgt ctaccgccac gttgttctcc accgtgacct ccaggtcata ggtcaccacg
     1081 ttgccttcgg agtcctgcat caggccgttg gcattcggct tgatcccgat ctccgccaga
     1141 agctccttgc cccgggccgg attgtacgtg tactgctccg caatggccgg atcgtagaag
     1201 gggttcggct ttgcgaagaa ggagagatcc ggatccgcca gccctcggta gacctccgtg
     1261 acaatgcggt cccggttcaa gaagcagctc atggcctggc ggaaggtcgt gttggagaac
     1321 cacttcaggt acttgggcgc caggttcttg gggttctggt tccacgagat gaacgcggcg
     1381 ccgagactcg ggcccgcgta gtacaccgag tagttgcggg ggtccttggc caccatgtcg
     1441 ctgaggtctt cgggtcggag gctgtagctg tccagctcgt tggcgaggaa cttgagcttc
     1501 tccgtgtccc ggttggggac gatcttcatc tcgatggtat cgatgtacgg aagctgctgc
     1561 ccgtagtcat ccttcttcca gtagttcggg ttgcgcacca gggtgacgcc aacgcccgga
     1621 cgataggact ggatatagta ctgtcccatg gacgggatgg tcttggggtc ggtatcgatg
     1681 gaccacaggt tcaggagtcc ttccacaccc ttggccttct tgaccggctc gaagatgtag
     1741 cgcgggccga agttcatgtt gctggagagc tccggcatcg cgacgatccg cggatagtgc
     1801 atcacgaacc gccggtcgtc gatcttctcc atgtcgatgt gcttcttgcc gccgtccggc
     1861 atctggagga actggcccga gtagccggac tgctgcaggg cctggtcccc gacgatgtcg
     1921 ttgtaccagt agaccacgtc gtccgacgta accttcacct tcttggaggg atccgccagc
     1981 gtggtccagt acaggtcatc ccgcagggtg aacgtcacgt ccatggtctg ggcctgatcg
     2041 ttgaccttca cctcgtacga ggcgacgttc ggcttccact gccgtttgta cgcgtcgtaa
     2101 tccaggaggt aatccgcgag aacgccgacc accgcgtccg tttcggtgtc ctgggagtgc
     2161 agcatgttga acgacttggg gtcgtcgttc agatcgctct gccacgtgcc gccgtgggcg
     2221 cctttcgggt acggcgccgt gctgcccggg cggttcaccg tcttggcgag aagggcgttc
     2281 tgctccactt ttttctggaa cgcgacctgg tccggcgtga gctgcgctac ccgggggccg
     2341 caggccgcaa gcgccgccag agccgtcacg gctaccacgg cgatgggggc tcccgccgac
     2401 gttctcatgg tacctcctgg ccgcgcgccc gcgcggcgtt tgcgcgccat cgatcgacta
     2461 gaggttactt cccgcccagt atcgcgggca aatcggacgc ggtcaaccgg gctctgcatc
     2521 atcgtcgctt gtcttcgtcg gcccgtcgac cgaccgccgg gaaccgggta tcttctcggt
     2581 catgagcccg gtgacattcc caaaaggttt tctctgggga gcctcaaccg ccgcctatca
     2641 gatcgaaggc tatcctctgg cggacggagc atcaccgtcc atctggcacg agttcgtcca
     2701 gcgcaccgga gctatccggg acgggactac gggtgacatc gcctgcgatc actatcatcg
     2761 ctacgcggag gatgtcggtg ccatgagcgc gctgggtctg aacgcgtacc gcttttcgat
     2821 cgcgtggccg cggatcctgc cggagcctgg ccgcgtgaat cccaaggggc tcgacttcta
     2881 ctcgcgcctg gtggactgtc tgctggaggc cgggatcaca ccgtatgcaa cactgtttca
     2941 ttgggacgtg ccgacctggc tcgaggagga gggcgggctc gtcgaacgga cgtccgtcga
     3001 tcacctcgtg cgttacgccg agacggtcgt gaagagtctt ggcgaccggg tccgacactg
     3061 gatcacgatc aacgagccga cgatcttcgc cctcttcggc tacatcacgg gggagtatcc
     3121 gccgggaagg acccgggacc tccgggcggc ctttcgggcc atccacttcc tcttgctcgc
     3181 tcacgcgcgg caggtccgag tccttcgggc cctcgccccc ggggcggagg tcgggcttgc
     3241 ccatcatttc gtctggatcg accctcgcga cccgcgtcgg cggcgggatg tccgggccgc
     3301 cacggagatg gacgattttg ccaaccacgc cgtcctggag agcctggtcc ggggcactta
     3361 cccaccgacc atcacctccc gggtaggccg cttcctcccc aaagggtttg aacgggatct
     3421 acaggaaatg gggatcccag gcgacttcat cggcatgaac tattacaccc gccagcagta
     3481 tcgccatgcg ctgctcatgc cttacctgca tgccagggaa tacaccgacc cgtcggcgcc
     3541 ccgaagcgcc atgtgggaga tacaccccga cggcatgtat cggtggctgg tccggctgcg
     3601 ggatcggtac ggcaatccgc ggtgtatcgt gaccgagtca ggattccccc tgccggatgt
     3661 ccaaggaagg gatccgctgg atgaccccga gcggatctct tacctcacgg accacatagc
     3721 ccgcatcggg aaggcgatcc aggagggagc ccgctgcgag gggtacttcc attggtccct
     3781 cctggacaac ttcgagtggt cctacggaac catcatgcgc tttggccttc tgcgaacgga
     3841 tttcgacacc cagaaacgga gctggaagcg gagtgcctac tggtaccgcg acctggttcg
     3901 cgcgaatgcg ctcccggcgg acggcgcctg acgtaaccgt tttcgtgccg tcgggtcggc
     3961 ctacgcgcac cgtcggacgg acgcgcgctc cacgatgttg aacggcatca ccaggctctc
     4021 ctggcgatgc cgtcggtcca ccaggatctt cacggctcgt ttgcccatct catagatcgg
     4081 ctgacgcacc gtggtaaggg gcggggtcgc catctcggcg tccggtgtgt cgttgtagcc
     4141 gataacggac atctgctcgg gcacacggat ctggtgggcg ccacaccacg ctatcgcacc
     4201 gaccgccatt tcgtcgctgg cagcgaagac ggccgttatt ccccggacct gagccatcag
     4261 gcggtccatc gcgtcgagcc cgctcttgaa ccggagatcg ccataggcta cgcgctcccc
     4321 tccagcatcg aggccctcct cccgaagcgc ccgcaggaac ccctcgacgc gcgtcacgcc
     4381 ggaaatggga tccttctgag gtccagcgat catgccgatg atgcggtgac cgttctctac
     4441 gaggtagcga gccgcatgaa acgccgctgc cgtgtcgtca acccggacat agggaagctg
     4501 atagcgcgtg gaggaagtag agacgagcac cgcaggaatc cccatccgct cgagctccct
     4561 gccgcactca tccgtgaggt tcgagccggc gaagatgacg ccgtcggcct gatgttcgcc
     4621 gagcacccgc aggtcgtgaa gggtgcggga atcatcatcg tccgtgttgc atacgattac
     4681 gctatggctc agctcgtggg ccccgtcctc gatcccgtgg agaaggagcg tcgagcagcg
     4741 gtcggagacg cgaggcagaa ggaccccgat cgtcgaggtc ctgcggctca ccaggccggt
     4801 ggccagcgcg ttccgacgga actccagtct atcgatgact tcgagcaccc gggcctttgt
     4861 ctcctcggta tacccgacac gtccgttcag gacgcgggac acggtagcca cggagacatt
     4921 cgcctctttc gccacgtctc gaatcgtcag attcgccatc ggttcacctt ctccaagtaa
     4981 acgattacgt cagcaatcgt agcgcggctt cgccaggatt tcaggctcgc cgaggggaaa
     5041 gtggcagtgt ttgtcgtctg cggcctcacg ttcagcgttc accgtcaaac gtgaacgggc
     5101 gatcgacaaa cgcctgtttg cgatttcttt ctctaacacg ctgttatttc cggaacgcgc
     5161 gatagattac ggcgaaggac aatcccactt gccaaccaat gaggaaggga gaacgtaatg
     5221 aggcgtaaac tcatacttct cgtgatggct gtcgccgtca cagcgttctt gttcatgctg
     5281 agtggatgcg atctgctcaa caagatcctg aacaacccgc cggtcgcgga tgcggggaag
     5341 gatcagaccg tccagatcga cactcaggtc acgctcgacg gtcacaacag cagggacccc
     5401 gacggcaacc agctaaccta tagctggtcc atcacgtctc agccgcctac ctccactgtc
     5461 aacaacagca aggtcagcgg cgcctcctct caggtcgcgt acttcacgcc cgatgttgag
     5521 gggctctata tcgccgagct gaaggtctcg gacggtcagg ctgactcgac ggacaccgtc
     5581 tccattacgg cgactgcctc ggggggtggc ggaggaggcg gaggcggtgg ctcagcgccg
     5641 tccgctccct cgatcaacgt tgcggccggc gacgcctacg tcaccgtaac ctgggcgggg
     5701 gtgagcgggg ccacctcgta caatctctac tacaagctcg gaggctccgt gacgatcgcc
     5761 acgggaacca agctgtcgaa cgtcacgtca ccgaaggatg ttacaggctt ggcgaatggc
     5821 tcgcagtacg ccttcattgt gaccgcggtc aacagcaacg gggaaagcgg ggcgagcaac
     5881 accgccacgg caaccccggt gctcgggatg ccgtccgctc ctaccggtct cggcgtcacc
     5941 acctcgggca accagctcca ggttacctgg ggcgccgtgg ccggcgccat caactacttc
     6001 gtgtacaagg acaccaatcc taccggttcc tatgccagca tagcgtacgg cggtggaaac
     6061 acgagcttca cggacggtcc ccttacgagc ggaaccacgt tctactatga agttgccgta
     6121 cagaccaaca tcggtacgag cggcaagtcc gcctatgtgg ctgggacgac cgtcccggca
     6181 gccccgagca ttcagtccgt ggccgcggga gagtcccagg ttgccatcac ctggggcaac
     6241 gtggccggag caaacagcta caacctgtac tgggcagcag gatcgacggc aactaccgga
     6301 agcaacaaaa tctccggcgt cggatcgccc tacacggtga ccgggctgtc ggacggcacg
     6361 cagtatgcgt tcatcgtcac tgcggtcaac gcgggcggcg agagtctccc gagcgcggcg
     6421 cagaccgcca cgcctcagat gggtattccg acgtcgcctt ccagccttct gatcacggga
     6481 gccagcggaa ccacgttgtc cctctcctgg agcgccgttg gcggcgccac cggctaccag
     6541 ctcttccggg acacgagctc gggtgggtct ttctcgaccc agaggtactc cgggagcgct
     6601 cttagcttca cggatagcaa tctgacccaa ggtacgacct actactacaa ggtccgggcc
     6661 agcaacggcg ctgggaccag ccctctctcc aactggcagt ccgggaccac gatcccctcc
     6721 tctccgaccg cctcggcggt tccgggagac gggcaggtca cggtgagctg ggccgcggta
     6781 cccggcgcta ccagctacaa cgtctactac gcagccggaa gctctgtcac catcggcgga
     6841 gcaggggtga caacggtact caacgccaac tcgccctgcc tcgtcagcag cctgacggat
     6901 ggcatccagt atgcgtttat cgtcacggcc gttaacggcg gcggcgaaag cgcggcgagc
     6961 agcccggcca cggccacccc gcagccgggt ctgccatcca ctccgcagaa cctctgggtg
     7021 ccgtcggcga cggcgaccag cctcacggtg cagtgggacg cggttagcgg cacgggtgtc
     7081 agctatgtgg tctaccgcgc caccgatcag gcgggcccct acgggttcca ggcctactcg
     7141 ggcaccagca ccagcttcac cagcaactcc cttgccagcg ggaccaccta ctactttgag
     7201 gtaaaggccc acaacgccag cggattcagc gccctttcga cgccttcctc ggggactacc
     7261 ctgcccgcgg cgccttcgat ctccgtgaca gccggcgatc agcaggtcac ggtctcctgg
     7321 gccagcgtcg ccggggcgga ctcctactac ctctactaca aggtcggggc cagtgtgacg
     7381 acggctaccg ggtcgatggt ggctggggtg acctcaccgt acatcatgcc cagcctcgcg
     7441 aacggcaccc agtacgcgtt catcgtgacg gcgtcggaca gcagcggcga aggcgcggcc
     7501 agcaacgtgg cgaccggcac ccccgcgcct ggtattccgc aggctccgtc cgggttgacc
     7561 gtgttcagcc cgaccggcac cagcctctcg gtggcgtgga actacgtaag cggggccacc
     7621 tcctaccagg tctatcgcga cacggctcag ttcggcacgt actctttcct ggcctacaac
     7681 gggaactcga ccgggttcac cgacaccggc ctctccagcg cgaccaccta ctactatgag
     7741 gtccgtgcga cgaatgcgag cggatccagc tcactgtccg cgccggtctc cgggaccacc
     7801 tccctcgtgg cgccgaacac acccgtcggg ctctccattg cgggcgtgac ggccaacacg
     7861 gtgacggtat cctgggtcgt gccgagcggc gcaacgagcg tccagctcta ccgggacacc
     7921 agccagctcg gttccttcag tctgctggtt tactcgggtt ccgcgtccag ctaccaggac
     7981 actgggctgt ccggcggaac ggactactac tacaaggtgc aggccaccaa cgacgcgggg
     8041 tccagctcga agtccgcctg gatcaagggc acgaccacca cgattgcgcc gagcacaccc
     8101 acgggtctgc tggtcgggaa cccgacctcg ggaagcctgg acgtgagctg gaacgcctct
     8161 cctggcgcca cctggtattc ggtcacccgg tcgacggacg cgaccttcgt ggtcagcaac
     8221 aacgtctaca acggcggagc caccggtttc acggatacgg gcgtggccag cagcacgact
     8281 tactactaca aggtcagcgc gggtaacagc gctggatcca gcttgcagtc cgcggcagcc
     8341 tcggggacga cccaggcctt gacgagcggg gaaggtgggt acggctatgg cattcgctaa
     8401 aggaaacgct tcgatgccgc gccttcacgc gacggaacag aaaggactga ggagaacgga
     8461 gatgaagcga agtatctacc tcctctcgat cgtggcgctg gcggccttca tcctcggatt
     8521 gggcgcctgc aacctcttcg actctcccct gacgccggta ccttcgggta ccggcaaggg
     8581 ggggatcacg atcaaactgc agggagtctc cccggcgatg cagcagctgg tcacccaggt
     8641 cgtgaagcgc aaccagccgg tctccggcaa agcgctctcg cccaagatgt atctcggcgc
     8701 gcagcgcgtc gagttcgagc tctgggacaa ccccccgacg agcgtgcagg acgcctggac
     8761 cgccagcctg gaccaggtca accccggcaa ctcgttcatc atcgggacga ggagccacgc
     8821 accgggcgac tatcagcttc gggcgtacgt ctacaacgac ccgaacgaca tcaccccgac
     8881 cgtcagcggg atcgagccgt tccatatcac cgccgaccag gatgccgcca gccaccaggt
     8941 cgtcgtgacc tgcttcccgg tgaacccggt cgtggttccc gagagcacgc cttcgctttc
     9001 ctatacgatg gccaagccct ggatcgtcaa cttcaataac ggcgtcatct cccaaatcgg
     9061 gtccgaacaa tggttccagg cgaccgcgca gtcggatcac acaacgttca ccgtgagccc
     9121 ggatgccgga agcggcgctc tgccggtgat catggtctgg gattcgaccg gacagatcgt
     9181 cggtcaggga atgcccaccg ccccgggcgt tgccactacg gccgggaaca tcccgaccac
     9241 gatcggcggc acctactact tcgccgccgc ggacttcagc gatccgacga ccccggtccg
     9301 ctcgttccgc gtgtcctacc agccgtgctc gatcaccgag gacctcgcgg tatcggtcac
     9361 gccggatacg acgggtagcc cggcctatac gggatactcc atccagacct tccacgcgca
     9421 gtggtactac ttcagcggcc tgcaagggca catttacacc gtcaactggg acgacgttgg
     9481 gaccggatcg aacctgtaca cgggagacgt ccaggtctcc gcgtaccgtc aggatcagag
     9541 cacgccctac ttccaggcaa tagacggcgg ttacgtgggc agctcgtact actccaacgg
     9601 caactactgg acgtactcca tcgatgagac gcagacgttc acaccgcaga ccagcgagct
     9661 cgtgtacatc aaagtcgagg aacgcaacgc gggcgccacg gcaggaacgt acggtctgcg
     9721 gatcgaggac ctgggtacca atcacccgcc ctgggcttac gcgtggacca acagcagcag
     9781 cgtggggacg ggatcgccgg tatccatcgg cgtgaacgcg ggggaccctg acatgggaga
     9841 taccctcacc tactcctggg tggtagcctc cgggccagac tacaacgggc tctccaacgc
     9901 caaccagccg tacgcgacat tcacgccgga tgcggcgggt acctatcagc tgacggtgac
     9961 ggtaaccgac agcggtactc cgcagcacag cgcgtcctcc tcgtgctacg tcacggccgt
    10021 ctacaacgcg ccgcccgtga tcgactgggc ctacccctcc acggcggaaa taacgctgag
    10081 tggctcctcg gtcatctacc tgtactcggg cgctcacgac ccggatggac cttcggtgac
    10141 ataccagtgg cagctcacca gcgtgccttc cggatcctgg gcatcgatcg tgggggccaa
    10201 ccaggcgaac gccacgctgg ctccggacgt gccgggcgcc tatgagctgc aactcacggt
    10261 taccgacacc ctgggaggga agacgccctc ggccacgata accatcacgg tggacgccaa
    10321 ccccggcgcc ggtggaatcg gcgtagggat caactaggcc agaggaagga agagagagtc
    10381 atgaagatca agcgaagtct ctacatcctt ggggctgccg tcggggcctc catcctcgtg
    10441 ctggcctcgt gcaacccgat gcttgcgaat acccttgctc ctgccgcggc gcccgcctcg
    10501 gcagggcaca gctcccccac cgccggcaag ggtggtgtcg ggatctcggt tcaggcgatc
    10561 tcgcccgtgg tgaacaacgt gttccagaag cagcttctgg cccagggctc gtccaagggg
    10621 ctctcgtcca aggcgtatct cggtgccgga cgcgtggagt tcaccgtcta tgatgtcacc
    10681 gacacgtatg tcatcgatgg ctggaccgtg tatccgagcc agaacagccc ctcgcagggt
    10741 ggctacagcc tgggcgcctg gtacggggct ccgtacaacg gttacgtgct tcacgccaag
    10801 gtctacaacc agcccggaga tggcgttccg gcggtccaag gcgaaagtta cttcta
//
LOCUS       DASRZS010000686         7494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_758759, whole genome shotgun sequence.
ACCESSION   DASRZS010000686 DASRZS010000000
VERSION     DASRZS010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758759"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VFH83_16700"
     CDS             <1..982
                     /locus_tag="VFH83_16700"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6488069.1"
                     /translation="VAVTIKDLARICGVSVGTVDRALRNRSEIHPRTRERILTAAREH
                     GYKPNIVARNLKARRTWEIGLVVHDLENEFFAELVNAVQEVAWRQGYFLQIAVSRRDP
                     ARERAGLEHMAGRNVDGILLFPTSADNGFDDFLRRLDRPIVTIANRVSPAWPFVGLKD
                     RTIVREATRDVIRRGYRRLVFVGPFDAFAERINLYEIEERYAGLLEAVREAPGIESCL
                     LSGADYVEKVRALDFRETRTAVLCASDIFALEILRDLRTREVAVPRDVGLMGFDAIDA
                     LRYVVPSLSTVAYPVQEMGETAFSLLTQPPTGKDVAFIELEPRIVWGESL"
     gene            complement(1018..1839)
                     /locus_tag="VFH83_16705"
     CDS             complement(1018..1839)
                     /locus_tag="VFH83_16705"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6488070.1"
                     /translation="MPRVLSVLRGVILAALVLIVLIPLFWMAESSFMRKAEFGSIPTH
                     WLPQQPDAATYQRLVAEQHFPRYYLNSIIISAVVSVSCVFFSALTGFALSTKYTFAGK
                     NAFFSFILATMLLPFELIMIPLYIMMRTVGILNTYAAVIIPGLLNGFGVFLLRQAINE
                     IPDDFLDAGRIDGAHEIVLFGRIVIPLAMPVMAALFIFTFVGSWNSYYWPLVVINAEP
                     RRTLTLAVAYMQGFYSTDYPLVLAAAMLSSVPIMLVFVFSQRFFIQSMTLSGLKG"
     gene            complement(1840..2745)
                     /locus_tag="VFH83_16710"
     CDS             complement(1840..2745)
                     /locus_tag="VFH83_16710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6488071.1"
                     /translation="MTPSASLRRPGRAVFRRPWDRWGLLFLLAGLVYFLGIYIYPTLR
                     TILISFTDYNLFTPPRFNGLSNYATLFRQGVFLESVRVTVIYVVVIGALNYVLPFGIA
                     LGLVSTRRLVGIGRAVYFLPVILPWVVVSAIWRAFYGAPGPIGSILGLVGIPFPVNPL
                     TQPPTALSAIIVVALWRSFGFYVVLFLTGLFAIPEAYYEVADLDGANGIQRLRYVTFP
                     LMRSTSLFIATVNFIGAIKEFDPFQVITAGGPAHATYAVTLFIYETGFKFLRMGRAAA
                     ASLLLCAAIVAVTVLQFRIFRPART"
     gene            complement(2788..4074)
                     /locus_tag="VFH83_16715"
     CDS             complement(2788..4074)
                     /locus_tag="VFH83_16715"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6488072.1"
                     /translation="MKRFLLVLSLAVVLAAAGAWAAGPVNLTIWTHTHAPANAYIQKL
                     VAQYMSANPNVKISYEPAADNNTYEDKVLTALASGAGPDVFNVGLSAMYTFAGLGQLA
                     EVTPQVLRDMGYSSMDAFKSDYIPGIIDKLLYNGKLYSVYREFNIFGLMLNKLAFKRV
                     GLDATRDAPKTWDDLAALAKKLTIWNGDTLVQMGMYLPQWGSDWTFIPMQPLFTQNGA
                     SVLGSSGTSSAIDSDAAVRTFTYFRQLSRAENDAPLKFQGDIWAQFSLGNVGMYVGST
                     WTMPLLKDSAKGDPRFSPDNFVVVPYPKLPGADRVHAPIQALVWAVNAKTPNVSEAWK
                     FVQWMTSGPQAVGWYNEVGYLLPTKAMMDMGKENPDVAKFLTLANDADLLFANPKYNE
                     IGAAVYSAWQAALTTNDDVKGILTKTAQQINDALSG"
     gene            4337..6931
                     /locus_tag="VFH83_16720"
     CDS             4337..6931
                     /locus_tag="VFH83_16720"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HET6488073.1"
                     /translation="MEKLRVANGIWWIEIPEADLRILCGCPADSVKHLMKRGLIQEKQ
                     KGGVTFETGPNAILLSDTPSQKGAFANLSEFPVLQMLYRQGMIIPNHPNNTGRKPMLI
                     GLQDQVRSQSEYIFRGNYGLASEEELVAAGMSPAAAREAMRMKRWFAFGRVRKTEDLV
                     EMKVVSKAASELVPGVFVHRKGLNRYEFLYNGEAVEVDLNLGTQEEYQAPYDLGWRVI
                     RRDYFSVIHIGEGDGWNPSRPCMGSIVCFQGKLFLIDAGPNIMSSLAALGISVNEIEG
                     IFHTHCHDDHFAGLTSLVRSDRRLRSYAVPWVRASIEKKLSALMGLDARRFGRLFEVH
                     DLVPKEWTSVDGLEVRAVDSPHPVETTILFFRASWEHGFKTYSHLADLPSMEVLDQMT
                     TRDTNKDGMSPDSRESFLHELFEPADVKKVDAGGGIIHGKAGDFQADGSRKVIFSHLS
                     APPKEEDLEFGSTAAFGLSDVLIPVHHRTYLLRSAYRYLRTYFPGAPGHELDLFTNGW
                     LATFNAGTIMIKKGESNREIYLVLSGIADVIARGEGFRNRLSAGALTGELSGFLGEPS
                     QRTFRAASHVTALRIPCDLYREFISRNGLDQTIRRVHDNRRMLFNSQLFGDMTSFHAQ
                     SAIARVMVRRTLQKGELAPPSEAQELYLLASGSVTLLSAGMELETLSPGDHWGEGAVL
                     GGLPAICEARAEEACVYFAIPGETLAELPSVQWNLLESFDRRMKRLRTKFRFEWQDFY
                     SVGVPDIDEQHRTLFTMINELAECIEGSGISAACRDESAHLLEYTRFHFEHEEKMLAG
                     YGYTRLEEQRTEHARLLGQLGTLVAAGASGKAGAQSPSEFFKDWLIDHTLLEDRRLKV
                     FLSEKNAG"
     gene            complement(6952..>7494)
                     /locus_tag="VFH83_16725"
     CDS             complement(6952..>7494)
                     /locus_tag="VFH83_16725"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6488074.1"
                     /translation="PVFRRLIDHPNVRGVVEHLVGPGAILFQDMALIKPPYIGSEKPW
                     HQDDAYFSYAPLERIVGVWIALDEARAENGCMHVIPGGHRNSGLRHFHSSLDCEIVPG
                     RISAQQAVPIELPPGGALFFSGLLPHQTPVNRSPLRRRAVQFHYRGADTVKLEAGDYD
                     RVFVDPDGMPASCKAAPKGR"
BASE COUNT         1374 a         2353 c         2534 g         1233 t
ORIGIN      
        1 ggtggcagtg accatcaaag accttgcgcg gatctgcggg gtatccgtcg gtaccgtgga
       61 tcgggcgttg cgaaaccggt ccgagatcca tccccgcacc cgggaacgca tcctgacagc
      121 ggcccgggag cacgggtaca aacccaacat cgttgcgaga aacctcaagg cccggcggac
      181 ctgggagatc gggctcgtgg tccacgacct ggaaaacgag ttcttcgccg agctcgtcaa
      241 cgccgtacag gaggtggcct ggcgtcaggg ctacttcctt cagatcgccg tgtcccgccg
      301 ggaccccgcc cgcgagcggg ccgggctgga gcacatggcc ggacgcaacg tggatggcat
      361 cctcctcttt cccaccagcg cggacaacgg cttcgatgac ttcctgcggc gtctggatcg
      421 gcccatcgtg accatcgcca accgcgtatc accggcgtgg ccgttcgttg ggctgaagga
      481 ccgtacgatc gtccgagagg cgacccgcga cgtcatccga cggggatacc gtcggctcgt
      541 gttcgtcgga ccgttcgacg cgttcgccga gcgcatcaac ctgtatgaga tcgaggagcg
      601 ttacgccggt ctcctggaag ccgtgcgaga ggcgccgggg atcgagagct gcctgctctc
      661 cggagcggac tacgtggaga aggtccgtgc gctggatttt cgggagaccc gcacggccgt
      721 cctgtgcgcc agcgacatct tcgcgctgga gatccttcgc gaccttcgca cgcgtgaggt
      781 cgccgtgccc cgggacgtcg ggctcatggg gttcgatgcc atcgacgcac tgcgctacgt
      841 cgtcccgtcc ctgtccacgg ttgcgtatcc cgtccaggag atgggggaaa ccgcgttctc
      901 cctgctgacg cagccgccca ccgggaagga cgtggccttc atcgagctgg agccccggat
      961 cgtgtggggg gaatccctgt agccatcggc ccgcgcggtt tgcgcgcggc ggcgcttcta
     1021 ccccttcagg cccgaaagcg tcatcgactg gatgaagaac ctctgggaga agacgaagac
     1081 cagcatgatg ggaaccgagc tgagcatcgc ggcggccagc accagcgggt agtccgtgct
     1141 gtagaagccc tgcatgtacg ccaccgccag ggtgagggtc cgacggggtt cggcgttgat
     1201 caccacgagc ggccagtagt aactgttcca cgagcccacg aacgtgaaga tgaacagagc
     1261 ggccatgacc ggcatggcaa gcgggatcac gatcctgccg aagagcacga tctcgtgcgc
     1321 cccgtcgatc cgtcccgcgt ccaggaagtc gtccgggatc tcgttgatgg cctggcggag
     1381 gaggaacaca ccgaacccgt tgagcaggcc cgggatgatc accgccgcgt acgtgttcag
     1441 gatcccgacg gtgcgcatca tgatgtacag cgggatcatg atcagctcga agggcaggag
     1501 catcgtggcc aggatgaacg agaagaacgc gttcttgccg gcgaaggtgt acttggtgga
     1561 gagcgcgaac ccggtaaggg cggagaagaa cacgcaggac accgacacca ccgcggagat
     1621 gatgatgctg ttcaggtagt agcgcgggaa gtgctgctcc gccaccagcc gctgatacgt
     1681 ggcggcgtcc ggctgctggg gcagccagtg cgtggggatg gagccgaact ccgccttgcg
     1741 catgaaggag gactccgcca tccagaagag cgggatcagg acgatcagga cgagcgccgc
     1801 caggatgacc ccgcggagga cggagaggac cctcggcatt caggtcctcg ccggccggaa
     1861 gatccggaac tgcagcaccg tgaccgccac gattgccgcg cacaggagca gggacgctgc
     1921 cgccgccctc cccatgcgaa ggaacttgaa ccccgtctcg tagatgaaga gggtcacggc
     1981 gtacgtggcg tgggccggac ctccggccgt gatcacctgg aagggatcga actccttgat
     2041 ggcgccgatg aagttgaccg tggcaatgaa gagactcgtc gagcgcatga gcggaaaggt
     2101 gacatagcgg aggcgctgga tcccgttggc gccgtccagg tccgccacct cgtagtaggc
     2161 ctcaggaatc gcgaagagac cggtcaggaa gagcaccacg tagaacccga aggatctcca
     2221 cagggcgacc acgatgatgg cgctcagggc cgtgggaggc tgggtcagcg ggttcaccgg
     2281 gaatgggatg cccaccagtc cgaggatcga gccgatagga cccggggcgc cgtagaacgc
     2341 cctccagatc gccgatacca cgacccaggg gaggatcacc ggcaggaagt agacggcacg
     2401 gccgatcccg accagccggc gggtggatac cagtcccagc gcgatgccga acggaaggac
     2461 gtagttgagc gccccaatga ccactacgta gatgaccgtg acccggacgg actccaggaa
     2521 caccccctgg cggaacaggg ttgcgtagtt cgagaggccg ttgaacctcg gtggcgtgaa
     2581 gaggttgtag tccgtaaacg agatgaggat ggtccggagc gtgggataga tgtagatccc
     2641 caggaagtag accaggcccg cgagaagaaa gaggagcccc caccggtccc acggccggcg
     2701 gaacaccgcg cggccgggcc ttcgaaggga ggcggagggg gtcatccgaa ggtgggggct
     2761 cctcgcccac cctccgcctc tggagcctca gcccgaaaga gcatcgttga tctgctgagc
     2821 ggtcttggtg aggatgccct tgacgtcgtc attcgtggtc agggccgcct gccaggcgct
     2881 gtagaccgcc gcgccgatct cgttgtactt cgggttggcg aaaagcaggt ccgcgtcgtt
     2941 tgccagggtg aggaacttcg ccacgtccgg gttctccttc cccatgtcca tcatggcctt
     3001 ggtggggagg aggtacccga cctcgttgta ccagccgacc gcctgcggtc ccgaggtcat
     3061 ccactggacg aacttccacg cctcgctgac gttcggggtc ttcgcgttca cggcccatac
     3121 cagcgcctgg atcggcgcgt ggacccggtc cgccccgggc agcttgggat agggaaccac
     3181 gacgaagttg tccggcgaga agcgcgggtc tcccttggcc gagtccttga gcagaggcat
     3241 cgtccaggtg gatccgacgt acatgcccac gttgcccagg gagaactggg cccagatgtc
     3301 gccctggaac ttgagcggcg cgtcgttctc tgcccgggag agctggcgga agtaggtgaa
     3361 ggtccggacc gccgcgtcac tgtcgatggc ggacgacgtg ccggaggagc ccaggacgga
     3421 cgccccgttc tgggtgaaca ggggctgcat cgggatgaag gtccagtccg atccccattg
     3481 cggcaggtac atccccatct gcaccagggt gtctccgttc cagatcgtca gcttcttggc
     3541 cagggcggca agatcgtccc acgtcttggg agcgtcccgg gtggcgtcca gccccacgcg
     3601 cttgaaggcg agcttgttga gcatgaggcc gaagatgttg aactcgcggt agacgctgta
     3661 cagctttcca ttatagagga gcttgtcgat gatcccgggg atgtagtcgc tcttgaaggc
     3721 atccatggag gagtatccca tgtccctcag gacctggggg gtcacctccg caagctgccc
     3781 cagcccggca aaggtgtaca tggcgctcaa ccccacgttg aacacgtccg gaccggctcc
     3841 ggacgccagc gccgtcagga ccttgtcctc ataggtgttg ttgtccgcag ccggttcgta
     3901 gctgatcttg acgttggggt tggcggacat gtactgcgca accagcttct ggatgtacgc
     3961 gttcgccgga gcgtgcgtat gggtccagat ggtcaggttc accggacccg cggcccatgc
     4021 gcccgcggcc gccagaacga cggccaggga gagcacgagc aggaatctct tcatgcaaag
     4081 gcctccttcg gttaacggcc gatcatcacg gcacggcgct tccccggccg ctgctccccg
     4141 ttaacgataa cggacaggaa tttacaaagg cgtcattgcc agcgtcaatg gaaaaccgag
     4201 gtcggaccgt ctccgccgcc gtggccagcg tccgcggagc cggttgggcg ttcggcgggc
     4261 gccatactgg cgattggccc acgccgggcg tatacttgtg gacgggaggg gttcgagact
     4321 cgcgaaaggg gcggcaatgg agaaactccg ggtcgcgaac ggcatctggt ggatcgagat
     4381 accggaggcc gatctgcgaa tcctttgcgg ctgtccggcg gacagcgtga agcacctcat
     4441 gaaacgcggc ctcatccagg agaagcagaa gggcggcgtg accttcgaga ccgggcccaa
     4501 tgccatcctg ctctccgaca ctcccagcca gaagggcgcc ttcgccaacc tttccgagtt
     4561 ccccgtcctg cagatgctct accgccaggg gatgatcatt ccgaatcacc ccaacaacac
     4621 gggccgcaag cccatgctca tcggcctgca ggaccaggtc aggtcccaga gcgagtacat
     4681 ctttcgcggg aactacgggc tggcctccga ggaagagctg gtggcggccg gcatgagccc
     4741 cgccgcagcc cgggaagcca tgcggatgaa acggtggttt gccttcggcc gggtgcgcaa
     4801 gaccgaggac ctggtggaaa tgaaggtggt gagcaaggcg gcctcggagc tggtgccggg
     4861 ggtctttgtc caccgcaagg ggctgaaccg ctacgagttc ctctacaacg gagaggccgt
     4921 ggaggtcgat ctcaacctgg ggacgcagga ggagtaccag gcgccctacg acctgggctg
     4981 gcgcgtcatc cgccgggact atttctccgt gatccacatc ggagagggag acgggtggaa
     5041 ccccagccga ccctgcatgg ggagcatcgt ctgtttccag ggcaagctgt tcctgatcga
     5101 cgccggaccc aacatcatga gctccctggc agccctgggg atcagcgtca acgagatcga
     5161 agggatcttc catacccact gtcacgacga ccactttgcc ggcctcacct ccctggtccg
     5221 gtcggaccgg aggctgcgct cctacgccgt gccctgggtg agggccagca tcgagaagaa
     5281 gctttccgct ctcatgggcc tggatgcccg gcggttcggc cgtctcttcg aggtccatga
     5341 cctggttccc aaggaatgga cctccgtgga cggtctggag gtccgggccg tggactcgcc
     5401 gcatccggtg gagaccacga tcctcttctt ccgcgcctcc tgggagcacg ggttcaagac
     5461 gtactcccac ctggcggacc tcccctcaat ggaggtgctg gaccagatga cgacccggga
     5521 cacgaacaag gacggcatgt ctccggattc ccgggagtcg ttcctgcacg agctcttcga
     5581 gccggccgac gtcaagaagg tggatgccgg cggcgggatc atccacggca aggccgggga
     5641 tttccaggcg gacggctcgc ggaaggtcat cttcagccac ctgagcgcgc cgccgaagga
     5701 ggaggatctg gagttcggct ccaccgccgc cttcggcttg agcgatgtcc tcatccccgt
     5761 tcaccaccgc acgtacctgc tgcgctccgc gtaccgctac ctgcgcacgt atttcccggg
     5821 ggcgccgggg cacgagctgg acctcttcac caacggctgg ctcgccacct tcaacgcggg
     5881 caccatcatg atcaagaagg gggagtccaa ccgggagatc tacctggtgc tgagcggcat
     5941 cgcggacgtc attgcccgcg gtgaggggtt ccgaaaccgg ctctcggccg gcgcgctgac
     6001 cggcgagctc tccggattcc tgggggagcc gtcccagcgc accttccgcg ccgcaagcca
     6061 cgtcaccgcg ctgcgaatcc cctgcgacct gtatcgggag ttcatcagcc gaaacggtct
     6121 ggaccagacc atccgccggg tgcacgacaa ccgcaggatg ctcttcaact cccagctctt
     6181 cggggacatg acgtccttcc atgcccagag cgcgatcgct cgggtcatgg ttcgaaggac
     6241 gcttcagaag ggagagctgg ctcctccctc ggaggcccag gagctgtact tgctcgcttc
     6301 gggctccgtc acgcttctct ccgcgggcat ggagctggag accctgtccc cgggggacca
     6361 ctggggggag ggcgccgtgc tgggcggcct gccggccatc tgtgaggcgc gagcggagga
     6421 ggcctgcgtg tacttcgcca tccccgggga gacgctcgcg gagcttccca gcgtccagtg
     6481 gaacctcctg gagtccttcg accgacggat gaagcggctg cggaccaagt tccgtttcga
     6541 gtggcaggac ttctacagcg tcggggtgcc ggacatcgac gagcagcacc ggacgctctt
     6601 cacgatgatc aacgagctgg ccgagtgcat cgaggggtcg gggatctccg cggcctgtcg
     6661 agacgagtcg gcgcacctgc tggagtatac gcggtttcac ttcgagcacg aggagaagat
     6721 gctggccggc tacgggtaca cgcgcctgga ggagcagcgc acggagcacg cgcggctcct
     6781 ggggcagctt gggacgctgg tggccgccgg tgccagcggc aaggcggggg cgcagagtcc
     6841 cagcgagttc ttcaaggatt ggctcatcga ccacactctg ctggaagacc gccgactcaa
     6901 ggtgtttctg tccgagaaga acgccggata gcggacggtt ccgccgcgcg gctaccggcc
     6961 cttcggcgcc gccttgcagg acgccggcat cccgtccgga tcgacgaaca cccgatcgta
     7021 atctccggcc tcgagcttca ccgtgtcggc gccgcggtaa tggaactgca ccgcgcggcg
     7081 gcgcagggga gagcggttta ccggcgtctg gtgcggcagg agaccgctga aaaagagcgc
     7141 gccgccgggc ggaagctcga tgggtacggc ctgttgcgcc gagatgcggc ccggcacgat
     7201 ctcgcaatcc agggagctgt ggaagtgtcg caagccggag ttgcgatggc cgccggggat
     7261 gacgtgcatg cagccgttct ccgcccgggc ctcgtccaag gcgatccaca ccccgacgat
     7321 ccgttccagg ggcgcgtagg agaagtaggc gtcgtcctgg tgccacggct tctcggaccc
     7381 gatgtacgga ggcttgatga gcgccatgtc ctggaagagg atcgcccccg ggcccaccag
     7441 gtgctccacg acgccacgca cgttgggatg gtcgatcagc cgacggaaga cggg
//
LOCUS       DASRZS010000687         4332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_759254, whole genome shotgun sequence.
ACCESSION   DASRZS010000687 DASRZS010000000
VERSION     DASRZS010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759254"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..963
                     /locus_tag="VFH83_16730"
     CDS             292..963
                     /locus_tag="VFH83_16730"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HET6488075.1"
                     /translation="MAGRIAALVGDYYHKAGPMLEALETVARALSLSLEPFEDPLALS
                     WDRLQPYRALVVAREDRMAPPDGKAVWAGRRHETALAEYVQAGGALLVLHAGLASYPT
                     DGPWFETVRGAFQFHPSEHPRFSVRGRNAPSADASRHAVLEGFSPFEIQDEQYFVRVD
                     SARTTCLLDSASADYGTSCAAWAHGVGKGRVFCFTPGHNPPVLENPSYRSVLNRGFRW
                     ALNEG"
     gene            complement(1064..1528)
                     /locus_tag="VFH83_16735"
     CDS             complement(1064..1528)
                     /locus_tag="VFH83_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HET6488076.1"
                     /translation="MIDKPKVVNCPEQLTASIHLTVPRSEIRTVMGPGLREVNAALKA
                     QGIGTSGPWLTHHLKMDPKVFDFEICLPVEKPVSAVGRVKPGKLPARKVARTVYHGDY
                     EGLEKAWGELSAWVKAEGHKSAPDLWEVYTSGPEASSDPSAWRTELNQPLLD"
     gene            complement(1552..2028)
                     /locus_tag="VFH83_16740"
     CDS             complement(1552..2028)
                     /locus_tag="VFH83_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6488077.1"
                     /translation="MTTSRLEVIAPADEPIIVTHRFIKAPAALIFKAWTTPEHLKQWL
                     GPRDLVMISCEVDLRVGGGYRYVHRAPDGQEYGFHGSYLEIDPPHRMVCTFVFEMFPQ
                     NEAVDTLTLEERDGGTLVTTHSRHPSLEARNGHLAGGMERGMSDGYERLEALLQRT"
     gene            complement(2015..2377)
                     /locus_tag="VFH83_16745"
     CDS             complement(2015..2377)
                     /locus_tag="VFH83_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6488078.1"
                     /translation="MRQVVSPDRSLDAVFSALADPTRRAIVERLARGSASVMELAEPF
                     KLSQPAISKHLKVLERAGLISQEREAQRRPRHLEPARIEQLSRWVERYRHILEESYER
                     LETYLQKLQGKEDSHDHL"
     gene            2491..2820
                     /locus_tag="VFH83_16750"
     CDS             2491..2820
                     /locus_tag="VFH83_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HET6488079.1"
                     /translation="MCRNIKPLYNFDPPATDAEIRSASVQFVRKVSGFHQPSKANEAV
                     FNAAVDAVAAATGDLLRSLKTTAPSRNREEEAAKARARAAVRFGTATVDPHPHPHQHP
                     HAHPHPG"
     gene            complement(2890..3888)
                     /locus_tag="VFH83_16755"
     CDS             complement(2890..3888)
                     /locus_tag="VFH83_16755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET6488080.1"
                     /translation="MSETEWGVRDARGEWQPAELPKPSPMFRLPWKVWDVIKWLFAPE
                     SFLWPYNLLYALMAVAAWLWFTPGFDRTVRFQAGWIAELYLRNAVFLLVVAGGLHLRL
                     YMTRAQGSKYKYTNKWLAAHDSKFLFSNQTWDNIFWNYVSGVTIWTAWEAVTLWLYAN
                     HKIPYVGWREHPVYCVLLMIAIPFLRLIHFYWVHRLIHWKPLYNLSHFVHHKNVNIGP
                     WSGLSMHPIEHLLYFSGVLFHWIIPSNPVHAIYHLMHAGVSPALGHAGFHKLVTKKEK
                     GLMSDTYFHYLHHRYFTVNFGSEAVPLDKWFGSFHDGSPRAHQAMMDKRSRARKRA"
     gene            complement(3952..>4332)
                     /locus_tag="VFH83_16760"
     CDS             complement(3952..>4332)
                     /locus_tag="VFH83_16760"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HET6488081.1"
                     /translation="SPQAVREAAHYRISQWSSINQGSVQEMKSFLAEGVPIVAGLRVY
                     QDFRDLDRDNPVYDNDAGQRMADHAVVIIGYDDDRSAFEVLNSWGTSWGIDGYGWIAY
                     DFTLSVIQEAYVALDAQDGHGTPR"
BASE COUNT          742 a         1426 c         1410 g          754 t
ORIGIN      
        1 gataccagtg ccacgccgag ggtcctgccg cgtcttccct cgcgaggccc gcccgcccgc
       61 cggcccgtcg ccacagccgg gtccgtcatt ccagcctcct cgaagacccg agagattgca
      121 cgtttgtacg cttcggctgg actccgcagg ccgagtgtca cttccctatg ccgcggccaa
      181 atttagccat ggggggaacg ggtcgtccag gagcaaatcc gtgtctgtac caagcatgcg
      241 ctcccgtgtc ataaacggcc ctgggcgcta tcgagcgcca aggaggagcc gatggcaggc
      301 aggatcgcag ctctcgtagg ggactattat cacaaggccg ggcccatgct ggaggcgctc
      361 gaaaccgtcg ccagggcgct cagcctgagc ctggaaccgt tcgaggaccc gctggccctg
      421 tcgtgggacc ggctccagcc ttaccgcgcg ctcgtcgtgg cacgggagga ccggatggcc
      481 ccgccggacg ggaaggccgt ctgggccgga cgccggcacg agacggccct tgccgagtac
      541 gttcaggccg gcggcgcgct cctcgtcctc cacgcggggc tcgcctccta tcccacggat
      601 ggcccctggt tcgaaaccgt gaggggcgcg ttccagttcc atcccagcga gcacccgcga
      661 ttctcggtcc gcggacgaaa cgccccgtcc gccgacgcgt cccggcacgc ggttctggaa
      721 ggcttctcgc cgttcgagat ccaggacgag cagtacttcg tccgggtcga ttccgcgagg
      781 accacgtgcc tcctggacag cgcttcggcc gactacggga cctcctgcgc ggcgtgggcc
      841 cacggcgtcg gaaaggggag ggtgttctgt ttcacgccgg gccacaatcc gccggtgctc
      901 gagaaccctt cctaccgaag cgtcctgaac cgtggattcc gctgggcgtt gaacgaggga
      961 tagccaggcg gccctgcggc cgcctggcgg acgtctgtgc ggcgggcgtc tactcccggg
     1021 cgtctcctcc cgggtgcgca ccggtcggga gcgcgcgaaa ggcctagtcc agaagcggct
     1081 ggttcaactc ggtccgccag gcggaaggat ccgagctggc ctccgggccg gaggtgtaca
     1141 cctcccagag gtcaggcgcc gacttgtgcc cttccgcctt gacccacgcg ctgagctctc
     1201 cccaggcctt ctcgagaccc tcgtagtcgc cgtgatacac ggttctggcg actttgcggg
     1261 ccggcagctt gcccggcttc acgcgtccga ccgccgacac cggcttctcc acgggcagac
     1321 agatctcgaa gtcgaagacc ttcggatcca tcttgaggtg gtgggtgagc cagggacctg
     1381 aggtgccgat gccctgggct ttgagggccg cattgacttc ccgcaggccc ggacccatta
     1441 cggtacggat ctccgaacgc ggcaccgtga ggtggataga ggccgtgagc tgctcggggc
     1501 aattcacaac cttcggtttg tcgatcatct ctcgctcctt cctgaaatcc gctatgtgcg
     1561 ctgaagcagc gcttccagcc gctcgtagcc gtccgacatg ccgcgctcca tgcccccggc
     1621 caggtggccg ttgcgtgcct caagggacgg gtgccggctg tgcgtggtga ccagggtgcc
     1681 gccgtctctc tcttccaacg tcagagtgtc gacggcctcg ttctgcggga acatctcgaa
     1741 cacgaacgtg cagaccatcc ggtggggtgg gtcgatctcc aggtaggaac cgtggaagcc
     1801 gtactcctgg ccgtccggcg cgcggtggac gtatcgatag ccgccgccca cgcgcaggtc
     1861 tacctcgcag gaaatcatca cgaggtctcg cgggccgagc cactgcttca ggtgctccgg
     1921 cgtggtccat gctttgaaga tgagcgccgc gggagccttg atgaaacgat gcgtcacgat
     1981 gatcggttcg tcggcgggcg cgatcacctc gagcctagag gtggtcatgg gaatcctcct
     2041 tgccctgcag cttctgcagg tacgtctcga ggcgctcgta gctctcctcg aggatatggc
     2101 gatatctctc gacccaccgg gaaagctgct cgatgcgcgc cggctccagg tggcggggcc
     2161 tgcgttgggc ttcccgctcc tgggagatca gtcccgcgcg ctccagcacc ttcaggtgct
     2221 tggagatggc gggttggctg agcttgaacg gctccgccag ctccatgacc gacgcgctcc
     2281 cccgcgccag acgctccacg atggctcgtc tggtcgggtc cgccagcgcg gagaacacag
     2341 catccaggga tcgatcaggt gacacgacct gtcgcataac caaagggtta tatagccaaa
     2401 cggtaaagtc aagggaaatc ggatgcgccg ggaggttgcg gatttgacgg cgcggcgcgg
     2461 gagacggaca ttggggtagg agacacggac atgtgccgaa acatcaagcc tctgtacaac
     2521 ttcgacccgc ccgcaaccga cgctgagatc cgatcggcct ccgtccagtt cgtacgcaag
     2581 gtgagcgggt tccatcagcc ttcgaaagcc aacgaagccg tgttcaacgc ggccgtggac
     2641 gccgtggcgg ctgccacggg agacctgctc aggtccctca aaacaaccgc gccgagcagg
     2701 aaccgcgaag aggaggccgc gaaggcgcga gctcgagccg ccgtgcgctt cggcaccgcg
     2761 accgtcgatc cgcacccgca tccccaccaa cacccgcatg cccacccgca tccggggtga
     2821 cgggcgctcc ggcccgcgac gctctctcga ccggacgcga ccctcgtggt ccgcgcccag
     2881 gccagcgcgt tacgcccttt tccgggcacg actccgtttg tccatcatcg cctgatgcgc
     2941 cctgggcgag ccgtcgtgga agctgccgaa ccacttgtcc agcggcaccg cttccgaccc
     3001 gaagttcacc gtgaagtacc ggtggtgcag gtagtggaag taggtgtccg acatcaggcc
     3061 cttctctttc ttcgtgacca gcttgtgaaa gcccgcgtgc cccagggccg ggctgacgcc
     3121 cgcgtgcatg aggtgataaa tggcgtgcac ggggttggac gggatgatcc agtggaagag
     3181 caccccggag aagtacagga ggtgctcgat ggggtgcatg gaaagtccgg accatgggcc
     3241 gatgttgacg ttcttgtgat ggacgaagtg ggacaggttg tacaggggct tccagtggat
     3301 gagccggtgg acccagtaga agtggatgag gcgcaggaag gggatggcga tcatcaggag
     3361 cacgcagtac acggggtgct cccgccaccc cacgtacggg atcttgtggt tggcgtagag
     3421 ccagagggtc acggcctccc atgcggtcca gatggtgacg ccgctcacgt agttccagaa
     3481 gatgttgtcc caggtctggt tgctgaacag gaacttgctg tcatgggccg ccagccactt
     3541 gttcgtgtac ttgtacttgc tgccctgggc gcgagtcatg tacaggcgca ggtgcagccc
     3601 gccggccact accagaagga agacggcgtt tcgcaggtaa agctcggcga tccaccctgc
     3661 ctggaagcga acggtccggt cgaagcccgg cgtgaaccac agccacgcgg ccacggccat
     3721 gagcgcgtag aggagattgt acggccagag gaagctctcc ggggcgaaga gccacttgat
     3781 gacgtcccat accttccatg gcaatcggaa catcgggctc ggtttcggca gctcggccgg
     3841 ctgccactct ccacgagcat cccggactcc ccactcggtt tccgacatga ccgcaccccc
     3901 tgggcgggga taatgtaacc atggcgtccg ccgcacggcc accagtcagc gtcaccgcgg
     3961 ggtgccgtgg ccgtcctgcg cgtccagagc gacgtacgcc tcttgaatga cggagagcgt
     4021 gaagtcgtat gcgatccacc cgtagccgtc gatgccccaa gacgtccccc aggagttcag
     4081 gacttcgaac gccgatcgat cgtcgtcgta cccgatgatc accacggcgt gatccgccat
     4141 gcgttggccc gcgtcgttgt cgtagacggg gttgtcccga tccaggtccc ggaaatcctg
     4201 gtacacgcgc agaccggcca cgatgggcac tccctccgcc aggaagctct tcatctcctg
     4261 gaccgagccc tggttgatcg aggaccactg agagatccgg tagtgcgccg cctcccggac
     4321 cgcctggggg ct
//
LOCUS       DASRZS010000688         5989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4603773, whole genome shotgun sequence.
ACCESSION   DASRZS010000688 DASRZS010000000
VERSION     DASRZS010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4603773"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFH83_16765"
     CDS             complement(<1..477)
                     /locus_tag="VFH83_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6488082.1"
                     /translation="MNTTAIALRTEVRQSDLEAVRHITDSTGFFYPEEVDTAVELVED
                     RLAKGPRCGYHFLFAEQDGKTLGYTSFGPIACTKASYDLYWICVAGEARGKGLGTLLL
                     SESESAIARLGGRRIYIETSARPLYEPTRGFYVARGYTQEAMLEDYYAPGDAKVIYL"
     gene            complement(474..1484)
                     /locus_tag="VFH83_16770"
     CDS             complement(474..1484)
                     /locus_tag="VFH83_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET6488083.1"
                     /translation="MTVAIVYGRVAEDAPEDEKDVLAQAREVRDALVLLGHQTFDVPV
                     SLDLQASAELLSQRRPEIAFNITDTVEGRGSLIHLAPSLLSSLGIRYTGAPFEAILLT
                     SNKLLAKRSLAAAWIPTPAWTTARAALEGVPPFGPPFIIKSVWEHASIGMDDSAVVME
                     ASQLCGSLRRREERGDAVDGLFVEAYVDGREFNLALLARPGHREPDVLPPAEIDFVGY
                     PDGKPRVVGYKAKWEAGSFEYGNTPRRFDYPAEDTRLLSELRRLALACWEQFALKGYA
                     RVDFRVGWDGRPWVLEINTNPCLSSDAGFMAAASRAGLTREQVVGRILSDGLSAREVS
                     RS"
     gene            complement(1570..2850)
                     /locus_tag="VFH83_16775"
     CDS             complement(1570..2850)
                     /locus_tag="VFH83_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HET6488084.1"
                     /translation="MPVVQDLAELADFRTTLPPFGATRYPASSRARPFKKPSSTPPKF
                     AVSERSEQFRARFFPEASVSEWCDWRWQLRNRVRTLPQLEKILRLTEDERTAIARHTG
                     SLPFAITPYYLSLIDPQDPEQPLRRTMVPVTDEYLLTRGEAEDPLEEDRDSPVPGIVH
                     RYPDRALFLVANSCASNCRYCTRSRMVENPDARSIFFQAQWEQAIAYIESHPEVRDVL
                     LSGGDPLTLSDEKLDWLLSRLRRIPHVEFLRIGTKAPVVMPQRVTPSLLRVLRRYHPL
                     WMSIHFTHPEELTPETTEACARLADAGIPLGSQTVLLAGINDEVGTMKRLMHGLLKTR
                     VKPYYLYQCDPIVGSSHFRTPVSKGLEIIQGLRGHTSGYAVPHYVIDAPKGGGKIPIL
                     PEYVVGRDGDELVLRNYQGLQYRYPDPLGTTKTE"
     gene            3206..4528
                     /gene="hflX"
                     /locus_tag="VFH83_16780"
     CDS             3206..4528
                     /gene="hflX"
                     /locus_tag="VFH83_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET6488085.1"
                     /translation="MLSESSEAQAVPREPERFKAAVMVGVELKGQGGAWTLQDSMTEL
                     RRLADTAGLQVVGETYQKLAHPDPATWIGKGKVKELSELLASTDAGTVLFDGDLSPAQ
                     QGKLEKALGGARVVDRTRLILEIFSQHASTREGKLQVELAQYQYLLPRLSRLHDGLAQ
                     QTGGAGAGPVGLRGPGETQLELDRRQIRGRIGTLKKALEDVRTQRRQYRRQRERAGLP
                     VVALVGYTNAGKSSLLNALTGAGVLVQDKLFATLDPTVRRLELPGGREALLTDTVGFI
                     RKLPHDLVAAFRATLEGIEEAALIVHVADCSHPHAAEQVAAVDGVLATLQTEGVARLL
                     VWNKVDLLEHHAPPAAPGYPSDRAVAISAKSGENLPELLRRVERLLAEGLTRLSLLVP
                     YDRFDIVRLAYDQGKVLAREDTADGVRLRVSVPLGVGQKMAPFSKEED"
     gene            complement(4525..5274)
                     /locus_tag="VFH83_16785"
     CDS             complement(4525..5274)
                     /locus_tag="VFH83_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET6488086.1"
                     /translation="MSLISTLAPWQWALGVVCALLVGLSKTGILGLGILFVPLFALVL
                     PARMSTGALLPLLIVGDAVAVIWYRRHARWEHLLRLLPWALAGIVAGFFALGKIDDHQ
                     LRPILGVMILVLLAISVWRDLATRGKAEVPTTWWFAAVAGLLAGATTMMANAAGPIML
                     LYLLAMKLPKEEFIGTGAWFFAAVNWAKVPFSAGLGLITGQSLLLDATLAGGVLIGAV
                     VGILVANRLPQRLFAVGMQVLTAAAAVLLLF"
     gene            complement(5271..>5989)
                     /locus_tag="VFH83_16790"
     CDS             complement(5271..>5989)
                     /locus_tag="VFH83_16790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6488087.1"
                     /translation="SLLREDIPGQHVDGLTGLKSREYLRNELPRHFSEFRAQAKRMAL
                     LLVNIDHLSWVNDRLGQPRGDRLLKAAAGLVLDNIREGDIAIRFGGEEMLVVFGGDLN
                     AGITLGERLRQAQERGLKHGDSMKDVLAIAEAEKDPCGTFSVGVIDATAIPDLAPALD
                     RVQQSLRHAKRSRNTVVCFDPARAGQGLEPFSSFAEYRQARPAAVPPRRAGPGSAGPA
                     GSVTPASPAFASQEKPGSTT"
BASE COUNT         1017 a         1962 c         2070 g          940 t
ORIGIN      
        1 caagtagatc accttggcgt caccgggcgc gtagtagtct tccagcatgg cttcctgcgt
       61 gtagcccctg gccacgtaga aaccccgcgt gggctcgtag agaggccggg cggaagtctc
      121 tatgtagatc cggcggccgc ccaggcgggc gatggccgac tcgctctccg acagaaggag
      181 cgtgcccagt cccttgcccc gagcctcgcc ggcgacacag atccagtaca ggtcgtagct
      241 ggccttggtg caggcaatcg gtccgaagct ggtgtagccg agggtcttcc catcctgttc
      301 ggcgaacagg aagtgatagc cgcatcgcgg ccccttggcc agccggtcct cgaccagctc
      361 caccgcggtg tccacttcct cagggtagaa gaaccccgtg gaatccgtga tatggcgtac
      421 cgcttccagg tcagactggc gaacctccgt gcgaagcgcg atcgccgtag tattcatgag
      481 cgagatacct cccgtgcgct gagaccgtcg gacaggatcc tgccaacgac ctgctccctg
      541 gtcaaccccg ccctggaggc ggccgccatg aagccggcgt ccgaagaaag gcagggattg
      601 gtgttgatct ccagaaccca cggccgaccg tcccatccaa cgcggaagtc gacgcgggca
      661 taccccttca acgcgaactg ctcccagcag gccagcgcca ggcggcgcag ctccgagagg
      721 agccgggtgt cctcggccgg gtagtcgaac cgccggggcg tgttcccgta ctcgaacgat
      781 cctgcctccc acttggcctt gtagcccacc acgcgcggct tgccgtccgg atatcccacg
      841 aagtcgattt ccgccggggg cagcacgtcc ggctcccgat gacccggtcg ggcaaggagc
      901 gccaggttga actcccgccc gtccacgtac gcctccacga acagtccgtc gacggcgtcg
      961 ccgcgctcct cacgccttcg cagggatccg cagagctgcg atgcctccat cacgacggcc
     1021 gagtcgtcca tcccaatgga agcgtgctcc catacggact tgatgatgaa cggcgggccg
     1081 aacggcggca ccccttccag cgcggcgcgg gccgtggtcc acgccggcgt ggggatccac
     1141 gccgccgcca gcgagcgctt ggccaggagc ttgttggagg tgaggaggat ggcctcgaag
     1201 ggcgccccgg tgtagcggat gcccagggag ctcaggaggc tgggcgccag gtggatgagg
     1261 ctgccgcggc cctccacggt gtccgtgatg ttgaacgcga tctcgggacg cctctgggaa
     1321 agcagctccg cgctggcctg gagatccagg gaaacgggaa catcgaacgt ctgatgcccc
     1381 agcaggacca gggcgtcacg gacctcccgc gcctgggcca ggacgtcttt ctcgtcctca
     1441 ggggcgtctt ccgccacgcg gccgtagacg atggctacgg tcatgagtcg gcaccgcggg
     1501 gagagggagg ggcgtgaacg acgcgcgccg cgcccccgcg tctggcggag gcagcggcgc
     1561 caggttggct cattccgttt tcgtggttcc cagcggatcc ggatatcggt attgcagacc
     1621 ctggtagttc ctgaggacca gctcgtctcc gtcccggcca acgacgtact cgggcaggat
     1681 ggggatcttc cctcctccct tgggggcgtc gatgacgtag tgcggcaccg cgtatcccga
     1741 ggtgtgcccg cgaaggccct ggatgatctc cagacccttg ctcacgggtg ttcggaaatg
     1801 cgaggagccg acgatgggat cacactggta gaggtagtag ggcttgacgc gggtcttcag
     1861 caggccgtgc atcagccgct tcatggtccc tacctcgtcg ttgatcccgg ccagaagaac
     1921 cgtctggctt cccaggggaa tccccgcgtc cgccagacgg gcgcaggcct ccgtggtctc
     1981 cggggtgagc tcctccgggt gggtgaagtg gatgctcatc cacagcggat ggtagcggcg
     2041 gagcacgcgc aggagcgacg gggtgacgcg ctggggcatg accaccggtg ccttggtccc
     2101 gatgcggagg aactccacgt gcgggatcct gcgcagacgg gagagcagcc agtccagctt
     2161 ctcatcggag agggtcaagg gatcaccgcc ggagagcagc acgtcccgga cctcgggatg
     2221 ggactcgatg tacgcgatgg cctgctccca ctgcgcctgg aagaagatgc tgcgggcgtc
     2281 cgggttctcc accatgcggg agcgggtgca gtagcggcag ttggaggcgc aggagttggc
     2341 caccaggaac agcgcgcggt ccggatagcg atggacgatc cccggcaccg ggctgtcgcg
     2401 gtcctcctcc agcggatcct cggcctctcc ccgcgtgagg aggtattcat cggtcacggg
     2461 caccatggtc cgacgcagcg gctgctcggg atcctgcggg tcgatgaggc tgaggtagta
     2521 gggggtgatc gcgaagggaa gggagccggt gtggcgcgca atcgccgtgc gctcgtcctc
     2581 cgtcagccgg aggatcttct ccaactgcgg aagggtgcgc acgcggtttc gaagctgcca
     2641 gcgccagtca caccactccg aaacggatgc ctccgggaag aagcgcgcgc ggaactgctc
     2701 ggaccgctcc gatacggcga acttgggtgg ggtggaggat ggcttcttga aagggcgcgc
     2761 acggctggac gcggggtacc gcgttgcgcc aaaaggcggg agtgtagtcc ggaaatcggc
     2821 cagctcggcc agatcctgga ctacgggcat ggtacctgga ggttctgcct cctcggtact
     2881 gagttccctc gtttccattt ctgcacgtac tccttcgttc tggtgaacgt ctctcttggg
     2941 ggctacgccc ccatcgcggc cagccgccag tggctcgatg ggccgctggg ttcaggtccg
     3001 gggcctccgc aggctgtcac ggttctcgaa acgcgtgcgg ggtgatgatc ccctccctgt
     3061 cgctcgtaca ttgcaatgca tactatatgc tttgctcctt tgcaagggga tcgacgattt
     3121 cacggcgaaa aaaccggcgc tcccgcggcg ttgcccccac gagcgtatag cgctcggcgt
     3181 tcggctgcgc tatgatgggc gcctcatgct gagtgaatcg agcgaagccc aagcggttcc
     3241 tcgggaacct gaacgcttca aggccgccgt gatggtcgga gtggagctga agggacaggg
     3301 cggcgcgtgg accctccagg attccatgac cgagctgagg cgcctggcgg acaccgcggg
     3361 tctccaggtg gtgggagaaa cctaccagaa gctggcccac ccggacccgg ccacctggat
     3421 cggcaagggg aaggtgaagg agctttcgga gctgctggcc tccacggacg cgggcaccgt
     3481 cctcttcgac ggggatctct cccccgccca gcagggcaag ctggagaagg ccctgggcgg
     3541 agcgcgcgtg gtggaccgca cccggctcat cctggagatc ttcagccagc acgcctccac
     3601 gagggaggga aagctccagg tggagctggc gcagtaccag tacctgctgc cgcggctgtc
     3661 ccgtctgcat gacggtctgg cccagcagac gggcggcgcc ggagcggggc cggtcggtct
     3721 gcgcggccct ggtgaaacac agctcgagct cgaccgcagg caaatccgcg gacgcatcgg
     3781 gaccctgaag aaggccctgg aggacgtgcg gacgcagaga cgccagtacc gaaggcagcg
     3841 cgagcgggcc ggcctgccgg tagtggcgct ggtgggctac acgaacgcgg ggaagagctc
     3901 gctcctgaac gcccttacgg gcgcgggcgt cctggtccag gacaagcttt ttgccacgct
     3961 ggatccgacc gtacgacggc tggagcttcc cggcggccgt gaagccctgc tcacggacac
     4021 cgtaggcttc atccgcaagc tcccccacga cctggtggcc gcctttcggg ccacgctgga
     4081 gggcatcgag gaggccgcgc tcatcgtgca cgtagccgac tgctcgcacc cgcacgcggc
     4141 ggagcaggtc gcggcggtag atggggtgct ggccactctc caaacggagg gcgtggctcg
     4201 gctcctggtc tggaacaagg tggatctcct ggagcaccac gcgccaccgg cggcgcccgg
     4261 ctacccctcc gacagggccg tggccatcag cgcgaagagc ggagagaacc tccccgagct
     4321 cctgcgccgg gtggagcgcc tccttgcgga aggcctgacg cgcctttcgc tcctggttcc
     4381 ctatgaccgg ttcgatatcg tccgactggc gtacgaccag ggcaaggtgc tggcgcggga
     4441 agacaccgcg gatggcgtgc gcctgcgggt gagcgtgccc ttgggcgtgg gccagaagat
     4501 ggctccgttc tccaaggaag aggactagaa gagcaacagg accgcggcgg cagccgtcag
     4561 cacctgcatc cccaccgcga agagcctctg gggcagccga ttggcaacca ggataccgac
     4621 gaccgcgccg atcagcacgc cgcccgccag ggtggcgtcc agcagcaggg actgccccgt
     4681 gatgagcccg agccccgcgc tgaagggcac cttggcccag ttcacggccg cgaaaaacca
     4741 tgcccccgtg cctatgaact cctccttggg tagcttcatg gccagcaggt acagcagcat
     4801 gatggggccg gcggcgttgg ccatcatcgt agtggcgccc gccaggagtc cggccacggc
     4861 ggcaaaccac caggtggtgg gcacctcggc cttccccctg gtggcaaggt cacgccaaac
     4921 gctgatcgcc aggagcacca ggatcatgac gcccaggatg gggcggagct gatggtcgtc
     4981 gatcttcccc agagcgaaaa agcccgccac gattcccgcc agcgcccagg ggagcagccg
     5041 caggagatgc tcccaccgcg cgtggcgccg gtaccagatc acggccaccg catccccgac
     5101 gatgagcagg gggagaagcg cgcccgtgga catccgcgcc ggaagcacga gcgcgaagag
     5161 cggcacgaag aggattccca gtcccaggat gcccgtcttg ctgagaccca ccaggagagc
     5221 gcagacgaca cccagcgccc actgccaggg agcgagagta gagatgaggc tcacgtcgtg
     5281 gagcccggct tttcctggga ggcgaatgcg ggggacgcgg gcgtcactga tcccgcgggt
     5341 cccgcggacc cggggccggc gcggcgaggg gggaccgctg ccggtcgggc ctgccgatac
     5401 tcggcgaagc tggagaacgg ctccaggccc tgtccggccc gtgccgggtc gaagcagacc
     5461 acggtattgc ggctgcgctt cgcgtggcgg agcgactgct gcacccggtc cagggcgggg
     5521 gccagatccg ggatggccgt ggcatcgatc acgccgacgg agaaggtgcc gcagggatcc
     5581 ttctcggcct cggcgatggc aagaacgtcc ttcatcgagt ctccgtgctt gaggccgcgt
     5641 tcctgggcct ggcgcagccg ctcgcccagg gtgatgccgg cgttcaggtc gccgccgaac
     5701 accacgagca tctcctcacc gccgaatcgt atcgcaatat caccctcgcg tatattgtcc
     5761 agcaccagcc ccgcggccgc cttcaggagc cggtctcccc gaggctgccc caggcgatcg
     5821 ttgacccacg agaggtggtc gatgttcacc aagaggaggg ccatgcgctt ggcctgggcc
     5881 cggaactccg agaagtggcg cggcagctcg ttgcgcaggt actcccggct cttgaggccg
     5941 gtgagaccgt ccacgtgctg gccggggatg tcctccctca agaggctct
//
LOCUS       DASRZS010000689         6249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2301275, whole genome shotgun sequence.
ACCESSION   DASRZS010000689 DASRZS010000000
VERSION     DASRZS010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2301275"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /gene="recN"
                     /locus_tag="VFH83_16795"
     CDS             <1..1332
                     /gene="recN"
                     /locus_tag="VFH83_16795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET6488088.1"
                     /translation="LHGQHEHQSLLDLENHRRLVDRWAATEELAERFHGLYASLASLR
                     DRLSALVGDERERLRRIDLLTYAVTEIKALALKPGEEEELEKELAILMHHEKLFTLLE
                     AVREQTAESSGGVLATLRAARHSIDEASAIDPDLSKLSHQLEDAFYEIEDFAQNIRQY
                     LSRTEFSPGRLDEVQTRLQSIRALEKKYGDSIEAVLEYATNSEQELAGMENWEEEKLK
                     LTDQIASTEKSIAEAARQLSQRRTEASKDLEKRIEQELADLGMPKVRFRVMISDAARA
                     DGTPVLTPWGKDAIEFVISPNLGEPFKRLRAIASGGELSRVMLAIKGVLADSDHVSTL
                     IFDEVDAGIGGEVAISVGERLRALSRHKQVLCITHLATIAVRADNHLRIEKVLADGRT
                     VTRVERVTGGARQEEIARMLAGDRKGELSLQHAGELLARYAPRGDRGDGPS"
     gene            1329..2144
                     /locus_tag="VFH83_16800"
     CDS             1329..2144
                     /locus_tag="VFH83_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6488089.1"
                     /translation="MRKPSDEARQRYAEYVKDYKAAIETVLQKEKQYKESAAKDPAGV
                     NYQRLALAEENINLVASYLLMNSLSQSFLGVKNENYLNESRKCIYRALIYLEEIVSPL
                     VDAPFSDYEAGLATISSFTDENRYNLVRKLGFAIDSIEQEYGEGTKWKWSFVELEGRF
                     AAVTKNLLNLKTFIAGMDPRVEGYSSRLAHLALARELLAQAADRYREKYELSTMRIDD
                     IKLGISFLAAVRRIHIVLGEADHADQVKKKADIWRAKMEADIKRSEETVKKQG"
     gene            complement(2208..2621)
                     /locus_tag="VFH83_16805"
     CDS             complement(2208..2621)
                     /locus_tag="VFH83_16805"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET6488090.1"
                     /translation="MQPPDPKDLLARARAAARSTYSPYSLFPVGAALACADGSVVTGA
                     NVENRSFGLSNCAERTAIFTAISQGKRDFVAIAVCCLASPTPVPPCGACRQVLSEFCP
                     PDMPVHYAGKEEAHVTVTLGELLPRDSLHGLKRSR"
     gene            2667..3818
                     /locus_tag="VFH83_16810"
     CDS             2667..3818
                     /locus_tag="VFH83_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6488091.1"
                     /translation="MNRSKSARLMLFAPIVIILYLLLFPYPLGRELVAVPRWAASLQA
                     PDQPGAASTASGDAQEAAPGAGVFAAPGAGLYAAPFRLGDLFGFVSPAGALVHLEREL
                     YQVTIAPTGFVSFGRLDTDWILRGPTGDPQGAYSSHGYPLLGAEGRRLLAVKTDLSGV
                     QELAPTGDVLWSRDFPAPVTSASVEADLTAAGLLNGSLLVIDRQGAVVLDARPGPSRI
                     RAIYGVSLSRDGKMLAAVSGIDPQRLTLWRWSGISFRVVESRPLPTSFRREVRIAFSP
                     NGRILLLEGNAAVGAYRTVSHRLTWLPVPGAVRDVSFLEPEGTVALTAGSETANQLML
                     FEPPEGPILRVPVPVGVSLSTVKGQLLLGVPALPQGNPPQLLRLDVEAR"
     gene            3815..4720
                     /locus_tag="VFH83_16815"
     CDS             3815..4720
                     /locus_tag="VFH83_16815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET6488092.1"
                     /translation="MNGRRTLVVLLPVALCLALSGAVSALDWPVAQPVVTGTFGEYRG
                     DHFQNGTDIGGGAQPVHPVLPGELVFRYDEGSDYSSVPRGTGSFVAIQHAQDLLTVYN
                     HLSKGSLGAETDSVMPQDTIGTIGDTGDATGSHLHLAFYDEQIASFVNPLAILPPVAD
                     HQKPVIRRVLLSIGDTLVPTPTQSAVGVDPQPAIAAGRYQILAQLYDPRQDVAYNWPM
                     APYSVRVSLDGKEIRAIVFDSLRVKDGRAVLGATDLDLDAVYRPDGLMELGTADLRSG
                     ESRLDIDVRDYAGNEAAFETQITIR"
     gene            4732..5643
                     /locus_tag="VFH83_16820"
     CDS             4732..5643
                     /locus_tag="VFH83_16820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6488093.1"
                     /translation="MPKTYSSVPAGERSETIPCPACGADDPRPYLDCGQFAFVRCGAC
                     SLVYQNPRPVFDDLRRRYDGHYFDYELSNEHAFFHLMRLGLKDIDFDRRTAGMEEPRT
                     FLDIGCATGMLIEWMAARGWRASGVDVCRESAEYGMAHRGVSISVGTLEEADFPDGSF
                     SVIHFSHLIEHVPDPGSFLTEVRRILKPGGFAVITTPNVDGFQARLFASGWRSAIADH
                     LVLFSRRTLESLLQARGFVVHQHVTWGGLAAGSAPALVKRPVDRLAKRWGFGDVVLFL
                     VQKPLVDIWEPGRGARSGARSRIAVTA"
     gene            complement(5633..5938)
                     /locus_tag="VFH83_16825"
     CDS             complement(5633..5938)
                     /locus_tag="VFH83_16825"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6488094.1"
                     /translation="MFERKVVSKDETVLVVDGMLTGDATSEFQRQMEEVVASGVPTVT
                     LDLSRTASINSSTLGKILLFRKKLAEQKRTLQIRGCSEGLYKTFQMIRFDTLISIKP"
     gene            complement(5951..6190)
                     /locus_tag="VFH83_16830"
     CDS             complement(5951..6190)
                     /locus_tag="VFH83_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6488095.1"
                     /translation="MLRTLTAVYPEGDTKELDRELSVNQLVDLNANPIALPLPSPRMI
                     VYRVWKITTHSERHTEEVLYHLELVTRPELEGVSR"
BASE COUNT         1056 a         2054 c         2081 g         1058 t
ORIGIN      
        1 ctgcacggcc agcacgagca ccagtctctc ttggacctgg agaaccatcg ccggctggtg
       61 gatcggtggg ccgccacgga ggagctggcg gagcgcttcc acggcctgta cgcctcgctg
      121 gcctctctgc gggaccgcct ctcggcgctg gtgggagacg agcgggagcg gcttcgtcga
      181 atcgacctgc tcacgtacgc ggtcacggag atcaaggcgc tggccctgaa gccgggggag
      241 gaggaggagc tcgaaaagga gctcgccatc ctgatgcacc atgagaagct cttcacgctc
      301 ctggaggccg tacgggagca gaccgcggag agctccggcg gcgtgctcgc gaccttgcgc
      361 gccgcccgtc attccatcga cgaggcctcc gcgatcgacc cggatctctc caagctgtcc
      421 caccagctcg aggacgcctt ctacgagatc gaggacttcg cccagaacat ccgccagtat
      481 ctttccagga ccgagttcag ccccgggagg ctggatgagg ttcagacccg gctgcagtcc
      541 atccgcgccc tggagaagaa gtacggggac agcatcgagg cggtcctgga gtatgccacg
      601 aacagcgagc aggagctggc gggcatggag aactgggagg aggagaagct caagctcacc
      661 gatcagatcg cctccacgga gaagtccatc gcggaggccg cccgtcagct ctctcagagg
      721 cggaccgagg ccagcaagga cttggagaag cgcatcgagc aggagctggc ggacctgggg
      781 atgcccaagg tccgcttccg ggtcatgatc agtgacgcgg cccgagcgga cgggacgccg
      841 gtgctcacgc cctggggcaa ggacgccatc gagttcgtga tctctccgaa cctcggagag
      901 ccgttcaagc ggctgcgggc catcgcctcc ggcggagagc tctcccgggt catgctcgcc
      961 atcaagggag tcctggcgga ctcggaccac gtcagcaccc tgatcttcga cgaggtggac
     1021 gccggaatcg gcggagaggt ggccatctcc gtcggggagc gcctgcgggc cctctcccgg
     1081 cacaagcagg tcctctgcat cacccatctc gcgaccatag ccgttcgcgc cgataatcat
     1141 ctgaggatcg aaaaggtcct ggcggacggg cgcaccgtga cccgcgtgga acgcgtgacc
     1201 ggcggcgctc gccaggagga gattgctcgg atgttggcgg gggatcgcaa gggggagctc
     1261 tcgttgcagc acgccggtga gctgctggct cgctacgcgc cccgcgggga tcggggggac
     1321 ggcccttcat gagaaagccc agcgatgagg ctcgacaacg ctacgcggag tatgtgaagg
     1381 actacaaggc ggccatcgag accgtgctgc agaaggagaa gcagtacaag gagtccgccg
     1441 ccaaggaccc cgcgggggtc aactaccagc ggctggccct ggcggaggag aacatcaacc
     1501 tcgtggccag ctatctgctc atgaactccc tgtctcaatc cttccttggc gtcaagaacg
     1561 agaactacct gaatgaatcg cgcaagtgca tctaccgggc cctgatctac ctggaagaga
     1621 tcgtcagtcc cctggtggac gcccccttct ccgactacga ggcggggctg gcaaccatca
     1681 gctcctttac ggacgagaac cggtacaacc tcgtgcggaa gctcgggttc gccatcgatt
     1741 ccatcgagca ggagtacggg gaggggacca agtggaagtg gtccttcgtg gagctggaag
     1801 ggcggttcgc ggccgtgacc aagaacctcc tgaacctgaa gaccttcatc gccggcatgg
     1861 atccccgggt ggaagggtat tcctcccgcc tggcgcacct ggccctggcg cgggagctcc
     1921 tggcgcaggc cgcggaccgc taccgtgaga agtacgagct ttccaccatg cgcatcgacg
     1981 acatcaagct gggcatcagc ttcctggcgg ccgtgcggcg gatccacatc gtgctgggag
     2041 aggcggacca cgcggaccag gtgaagaaga aggcggacat ctggagggcc aagatggagg
     2101 ccgatatcaa gcggtccgag gagacggtga aaaagcaggg gtgagggacg gtgacccggc
     2161 gccccgcggg gtcccctcgg agcccctcgg ctccgtcacg gggccatcta cctgctgcgt
     2221 ttcaggccat gaagcgaatc cctgggcaga agctccccca acgtcacggt gacgtgcgcc
     2281 tcctccttgc cggcgtaatg gacgggcatg tccggagggc agaactcgct cagcacctgg
     2341 cggcaggcgc cacagggggg aaccggagtg ggggacgcca ggcagcacac ggcaatcgcg
     2401 acgaagtcgc gcttgccctg tgagatcgcc gtgaagatgg ccgttcgctc cgcgcagttg
     2461 ctgagcccga acgaccggtt ctccacgttc gcgcccgtga ccacgcttcc atccgcgcag
     2521 gcgagcgcgg cgccaacggg aaagagcgaa tacggagagt acgtggagcg cgccgcggcg
     2581 cgcgcgcggg ccagaaggtc cttcgggtcg ggcggctgca tggtcagatc ccccagcaac
     2641 ggtatcgatc gtctcaggga ccgaccatga atcggagcaa gtcggcacgc ctgatgttgt
     2701 tcgctccgat agtgataata ctgtatctcc tgcttttccc ctatcccttg gggcgcgagc
     2761 tggtggctgt tccgcggtgg gcggccagcc tgcaggcacc ggaccagccg ggggccgcct
     2821 cgactgcaag cggggacgcc caggaggcag cccccggggc cggggtcttt gcagcccctg
     2881 gggcggggct ctatgccgct cccttccgcc tgggggatct cttcggtttc gtgagccccg
     2941 cgggagcgct ggtgcatctg gagcgggagc tctaccaggt aacgattgcg ccgaccggat
     3001 tcgtgagctt cggacggctg gacacggact ggatccttcg cggtcccacc ggcgacccgc
     3061 agggggcata ctcctcccat gggtatcccc ttctcggcgc ggagggaagg cgcctcctgg
     3121 ccgtgaagac ggacctgagt ggagttcagg aactggcccc gaccggtgac gtcctgtgga
     3181 gccgtgactt tccggctccg gtcacgtccg catcggtgga ggcagacctg accgccgccg
     3241 gtctgctgaa cggctcgctt ctggtgatcg accgccaggg ggcggtggtg ctggacgctc
     3301 ggcccggacc cagccggatc cgagcgatct atggggtatc gctctcgcgg gacgggaaga
     3361 tgcttgctgc cgtgtccggc atcgatccgc agcggctcac gctctggcgg tggtccggca
     3421 tcagcttccg cgtcgtcgaa tcccggcctc tccccacgag cttccgccga gaagtccgta
     3481 tcgcgttctc ccccaatggc cggatcctgc tcctggaggg gaacgccgcg gtcggagcct
     3541 atcggaccgt ctcgcaccgc ttgacctggc ttcccgtccc cggggcggta cgggacgtga
     3601 gcttcctgga acccgaaggt acggtggccc tcacggccgg atccgagacc gcgaaccagc
     3661 tcatgctctt cgagcctcca gagggcccca tcctccgcgt ccccgtaccg gtcggcgtct
     3721 cgctctctac ggtcaaaggc cagcttctcc tgggtgtccc ggcgctccct caggggaacc
     3781 cgccgcagct tttgagactg gacgtggagg cccgatgaac gggcggagga ccctggtggt
     3841 gctgctcccg gtcgcgctgt gcctggcgct ctccggcgcc gtgtccgccc tggactggcc
     3901 ggtggcccag cccgtggtca ccgggacgtt cggcgagtat cggggagacc atttccagaa
     3961 cggtaccgac atcggcggtg gcgcgcagcc ggtccatccc gtgcttcccg gcgagctggt
     4021 cttccgctac gacgaggggt cggactattc ctccgtgccc cggggtacgg gcagcttcgt
     4081 tgccatccag catgcccagg atctcctcac ggtctacaac catctcagca aggggagcct
     4141 gggtgccgaa actgatagcg tgatgccgca agacacgata ggcaccatcg gagacacggg
     4201 agacgccacc ggcagccacc ttcacctggc gttctacgac gagcagatcg ccagcttcgt
     4261 gaaccccctc gccatcctgc ccccggtggc ggaccaccag aagccggtga tccgccgcgt
     4321 gctcctttcc atcggggaca cccttgtgcc cactccgacg cagtctgcgg tcggcgtcga
     4381 tccccagcct gcgatagctg ccgggcgcta ccagattcta gcccagctct acgatccgcg
     4441 ccaggacgtg gcgtacaact ggcccatggc gccctacagc gtccgtgtca gcctggatgg
     4501 caaggagatc cgcgccatcg tttttgattc cctgcgcgtg aaggacggca gagccgtgct
     4561 gggcgccacg gacctggatc tggacgcggt atatcgcccg gacggtctga tggagctcgg
     4621 gaccgcggac ttgaggtcgg gcgagtctcg cctggacatc gacgtccgcg actacgccgg
     4681 caacgaagcc gccttcgaga cccaaatcac gatccgatag gaggtgggcg catgcccaag
     4741 acgtattcct cggtccccgc cggagagcgg tccgagacca tcccctgtcc cgcctgcggc
     4801 gcggacgatc ctcgtcccta tctcgactgt gggcagttcg ccttcgtgcg gtgcggggcc
     4861 tgctccctgg tataccagaa tccccggccc gtgttcgacg acctgcgcag acgctacgac
     4921 ggccactact tcgactacga gctctccaac gagcacgcct tcttccacct gatgcggctc
     4981 gggctcaagg acatcgactt cgatcggcga accgccggga tggaagagcc ccggaccttc
     5041 ctggacatcg gatgcgccac cggcatgctg atcgagtgga tggccgcgcg agggtggagg
     5101 gccagcggcg tcgatgtctg ccgagagtcc gcggaatacg gcatggcgca tcgaggggtt
     5161 tccatctccg tgggcaccct ggaagaggcg gatttcccgg atggctcttt ctcggtgatc
     5221 cacttctccc acctgatcga gcacgtgccg gatccgggaa gcttcctgac ggaggtccgc
     5281 cgcatcctga agccgggcgg cttcgcggtg atcaccaccc cgaacgtgga cggctttcag
     5341 gcgcggctgt tcgcgtccgg ctggcgatcc gccatcgccg accacctggt gctcttctcc
     5401 cgccgcaccc tggagagcct gctgcaagcg cggggctttg tcgtgcacca gcacgtgacg
     5461 tggggtgggc tggctgcggg aagcgctccg gcgctcgtga agaggcccgt ggatcgcctg
     5521 gccaagaggt ggggattcgg cgacgtggtt ctcttcctgg tccagaagcc cctcgtggac
     5581 atctgggagc cgggacgggg agcgcgctcc ggcgcccggt cgaggattgc ggttacggct
     5641 tgatcgagat gagggtgtcg aaccggatca tctggaaggt cttgtagaga ccctcgctgc
     5701 agccccggat ctgcagcgtc ctcttctgct cggcgagttt cttgcggaac aggaggatct
     5761 tgccgagcgt ggaggagttg atggacgccg ttcgcgagag gtccagggtg accgtgggca
     5821 ccccggacgc cacgacctct tccatctgcc gctggaactc gctggtcgcg tccccggtga
     5881 gcatcccgtc caccacgaga accgtctcgt ctttggaaac caccttgcgc tcaaacatcg
     5941 gtccgacccc ctatcggctc acgccttcca gctccggccg agtcaccagc tcgaggtggt
     6001 agagaacctc ctccgtatgg cgctcgctgt gcgtcgtgat cttccagacc cggtagacaa
     6061 tcatgcgcgg gctcggcaac gggagcgcga tcggattggc attcaggtcc accaactggt
     6121 tcaccgaaag ctcccggtcg agctccttgg tgtccccttc gggataaacc gcagtcagcg
     6181 tccgcagcac gatgccctac tgtatcccgc gaccgcccgg acgttcaatc ggtgtggtcc
     6241 gatcccgct
//
LOCUS       DASRZS010000690         4804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_2306600, whole genome shotgun sequence.
ACCESSION   DASRZS010000690 DASRZS010000000
VERSION     DASRZS010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2306600"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VFH83_16835"
     CDS             <1..896
                     /locus_tag="VFH83_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6488096.1"
                     /translation="APIAVIGLLVVASLIAAAEAAGAAGTAGAADPGLTTTSAVDWAA
                     GALVVDVSKVLDPSIPALPKAKETAQADIADRLSGILTLALGPVLVDSSHTFGQAVAS
                     DPSVFQAVAELVAAVSPRQAALTRDLSTLTVRYSLPFFGAGGIALPLLPTHGVAPRRR
                     LGFVATRRYTGVLIYAKGPLSYVGTPETRLAQPALFPRLWDEQMNLVLDKGMCNPDDL
                     GRWGMVGYAQSLDDSATLRAGASPLRITARGVFGDNATDIVISDDAAAQLLSLPENIG
                     MLQQGRIVIVYESLPGPPSTP"
     gene            complement(886..2112)
                     /locus_tag="VFH83_16840"
     CDS             complement(886..2112)
                     /locus_tag="VFH83_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HET6488097.1"
                     /translation="MDAHASLRRKIAEKRETIIGLQRALTAVPALGPQNGGTGEWDKA
                     QVLISWLDRLGLGPAEMHAAPDPRVPRGERPNCLVTLPGASDASTFWIMTHLDIVPPG
                     EASLWESDPYGMVVKGDHLVGRGVEDNQQALVASVFAAAAVKELGLKPRRTVKLLFVS
                     DEETGSQLGVQHLLSHTELFRTQDVALVPDGGCEDGSEIEIAEKSLLWLRFRLRGRQC
                     HASTPEKGINAFLAGSHLVVRLGELATRYPAHDDLFSPPISTFTPTKKEANVPNVNTL
                     PGEDVFYLDCRVLPREDLDEVLQVIREICDGVQRDFGVDITFEAVQRNSSPATPADAP
                     FVAALSRAVKQVYGVEARTVGIGGGTVGAFLRRAGIPTAVWSRVLETAHQPNERCSLP
                     NLMGDALVMALLMLME"
     gene            2302..3603
                     /locus_tag="VFH83_16845"
     CDS             2302..3603
                     /locus_tag="VFH83_16845"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET6488098.1"
                     /translation="MSRRHLVAALAALAALLLASCGVKAVGYGVVLWGGSDGAPPTGV
                     VVPVIRESPVNSSYLISVQGQKQLGEIPMGRLRFFKKRADADTYARGYSAFLPSWALV
                     MKLDGNPLPIRDAASQDGKLVYRLQPKQLIKVISRSAAPVTVPPYTDYWYEVATEDGS
                     SGWTFGHYLKLFSVQGDPSSEADRLRNQDDNLDRLMSTVWRPGWFQDQIAAGAIDLTM
                     LRDDVGLFPDPANKTVKLVLPLSTLEFHYDSIQNVGPAAYNFLGADLRVSMMDQTTIE
                     VQYHLKGQLVRAVYVDLDADVSQLVANEQKRRSDLFDGLVQHGATLTSSAYGTIRLQT
                     GMRFTWEGFGRLVPTLIGPSAKGAGSVDFPLHVGRHLTGTYDGVITLTFDEYPQSPVN
                     FLYKSTDNGLRLTSVAAGGIQDLIVNAEGISPVVIFFTVSP"
     gene            3607..>4804
                     /locus_tag="VFH83_16850"
     CDS             3607..>4804
                     /locus_tag="VFH83_16850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET6488099.1"
                     /translation="MPLVSLSGVGLSYGARTLLDSVNLTVATGTRMALVGPNGSGKTT
                     LMRIMASQVRADIGSVVVERGTRVSYVPQSGVVHAGSSLREEAEKAFSYGAGIEAELA
                     ALQERLGTISQGDPQAEGLLWKHHELQERLEASGYHRRDEAIQRILSGLGFSLEDLEK
                     PSSSFSAGWQMRIALARAILETPDILLLDEPTNYLDLEARDWLEGHLAAFPGGLLLVS
                     HDRYFLDVVVTAVAEIYMARVSVVTGNYTHYEERRAQELAAIVERWKQQQEEIARAES
                     FITRFRYKASKARQVQSRITALEKIERIEIPPVVKGIHFTFPKPPHSGQIVLTATGLA
                     KSYGGRTVFRGVDVEVTRGEKLVVVGVNGAGKSTLLRLISGREAPDEGSLRAGTGVVP
                     AYYSQEN"
BASE COUNT          756 a         1615 c         1638 g          795 t
ORIGIN      
        1 gagctccgat cgcggtcatc ggcctgctgg tggtcgcgtc cctgatcgcg gccgcggagg
       61 cggcgggcgc cgcgggtact gccggtgccg cggaccccgg gttgacgacg accagcgccg
      121 tggactgggc cgccggcgcg ctcgtggtcg acgtaagcaa ggtcctggac ccctccatcc
      181 ccgcgctgcc gaaagccaag gagaccgccc aggcggatat cgccgaccgg ctgagcggga
      241 tcctgacgtt ggcactcggt ccggtcctgg tggattcctc ccataccttc ggccaggcgg
      301 tggcgtcgga cccttcggtc tttcaggccg tggctgagct ggtggcggcg gtgagcccgc
      361 ggcaggcggc cctcacgcgg gacctctcga cgctgacggt ccgctactcc ctgccgttct
      421 tcggagcggg ggggatcgcg ttgccgcttc tcccgaccca cggggtggcg ccccgccgac
      481 ggctgggctt cgtggccacc cggcggtaca cgggcgtgct gatctacgcg aagggccccc
      541 tctcctatgt gggcaccccg gagacccggt tggcgcagcc ggccctcttc cccaggctct
      601 gggacgagca gatgaacctg gtgctcgaca aggggatgtg caatccggac gacctggggc
      661 gctgggggat ggtgggctac gcccagtcgc tcgacgactc cgccaccctg cgggccggcg
      721 cctcgcctct gaggatcacc gccagaggcg tgttcggcga caatgccacc gacatcgtca
      781 tctccgacga tgccgcggcg cagcttctct ccctgccgga gaacatcggc atgctccagc
      841 aaggcaggat cgtgatcgtg tacgagagcc tgcccggccc gccttctact ccatgagcat
      901 cagcagggcc atgaccagcg cgtctcccat gagattggga agggagcagc gctcgttggg
      961 ctggtgagcc gtttccaaca cgcgggacca gaccgccgtg gggatgccgg cccgacgcag
     1021 gaacgcgcca acggtgccgc cgccgatccc gacggtgcgg gcctccacgc cgtagacctg
     1081 cttgaccgcc ctgctcaagg ccgcgacgaa cggcgcgtcg gcgggcgtgg ccggggatga
     1141 gttgcgctgg acggcctcga aggtgatgtc caccccgaag tccctctgca ccccgtcaca
     1201 gatctcccgg atcacctgga gcacctcgtc caggtcctct cgcggcagca cccggcagtc
     1261 caggtagaac acgtcctctc ccggcagcgt gttgacgttc gggacgttgg cttccttctt
     1321 ggtaggagtg aacgtcgaga tgggagggct gaacaggtcg tcatgagcgg ggtaccgggt
     1381 ggcaagctct cccagccgca ccacgaggtg gcttcccgcc aggaaggcgt tgatcccttt
     1441 ctccggcgtg gaggcgtgac attggcgtcc gcgcaggcgg aaccgcagcc agaggaggct
     1501 cttttccgcg atctcgatct ccgagccgtc ctcacaaccg ccgtccggca ccagcgcaac
     1561 gtcctgcgtt ctgaagagct ccgtgtggga gaggaggtgc tggacgccga gctgggagcc
     1621 tgtttcttcg tcggagacga agagcagctt tacggtgcgc cggggcttca gccccagctc
     1681 cttgacggcg gccgccgcga acacggaggc caccagcgcc tgctggttgt cctcgactcc
     1741 gcggccgacg aggtggtcgc ccttcaccac catgccgtac gggtcggact cccagaggga
     1801 ggcttccccc ggcgggacga tatccaggtg cgtcatgatc cagaaggtgg aggcatccga
     1861 ggcgccgggc agcgtgacca ggcagttggg tctctccccc cgaggcacgc gcggatccgg
     1921 ggcggcgtgc atctccgcgg gaccgagccc cagccggtcc agccaggaga tcaggacctg
     1981 ggctttgtcc cactcccccg ttccgccgtt ctgcggaccc agcgcaggga ccgcggtgag
     2041 agcacgctgc agtccgatga tagtctcccg cttttcggcg atcttgcgcc tcaggctcgc
     2101 atgggcatcc atccatgtcc tcctggccag ggatataagt cattttgagc ctcggggcaa
     2161 gggcggcggg ccgtgcgggc ggcggatcgt agcggggtcg catgccttac ggcgagcgag
     2221 cgggctgtgc ggggagcgcc atgccgaagc ccgcacgcgc tccccttgaa caattcggat
     2281 gcgatccgca gtatacgatc catgagccga aggcatctcg ttgccgcgct ggcggcgctg
     2341 gcggcgctcc tgctggcttc gtgcggcgta aaggcggtgg gctacggcgt ggtcctgtgg
     2401 gggggctccg acggggcccc gcctaccggc gtggtggtcc cggttatccg ggagtccccc
     2461 gtcaactcgt cctatctcat cagtgtgcag ggccagaagc agctcggcga gatccccatg
     2521 gggcggcttc gcttcttcaa gaagcgcgcg gatgccgaca cctacgcccg cggctattcg
     2581 gcgttcctgc cttcctgggc tctcgtgatg aagctggacg gcaacccgct tcccatccgg
     2641 gacgccgcct cccaggacgg gaagctggtg taccggctgc aacccaaaca gctcatcaag
     2701 gtgatcagcc gctccgcggc cccggtcacg gtgcctccct acaccgacta ctggtacgag
     2761 gtggccacgg aggacgggtc ctccggctgg accttcggcc actacctgaa gctcttctcc
     2821 gtccaggggg atccgagctc cgaggcggat cggctgcgca accaggacga caacctggac
     2881 cgcctgatga gcaccgtgtg gaggccgggc tggttccagg accagatcgc cgcgggggcc
     2941 atcgacctga cgatgctccg cgacgacgtg gggctgttcc cggatccggc caacaagacg
     3001 gtgaagctgg tccttccgct ctccacgctg gagttccatt acgactcgat ccagaacgtg
     3061 ggcccggcag cctacaactt cctgggcgcg gatctccggg tcagcatgat ggaccagacc
     3121 accatcgagg tgcagtacca cctcaaaggt cagctcgtgc gggcggtgta cgtggacctg
     3181 gacgccgacg tatcccagct tgtggcgaac gaacagaagc ggcggtccga cctctttgac
     3241 gggctggtgc agcacggcgc caccctgacc tccagcgcgt acgggaccat ccgtctgcag
     3301 acgggaatgc ggttcacctg ggaaggcttc gggcgcctgg taccgaccct gatcggtccg
     3361 tccgccaagg gcgccggttc cgtggacttc cctctccacg tggggagaca cctcacgggc
     3421 acctatgacg gcgtgatcac attgacgttc gacgagtatc cgcagagccc cgtcaacttc
     3481 ctgtacaagt ccacggacaa cggtctgcga ttgacctccg ttgcggcggg cggcatccag
     3541 gacctcatcg tgaacgcgga agggatctcc ccggtggtga tcttcttcac ggtgtcgccc
     3601 tagccgatgc ccctcgtaag cctctccggg gtgggcctct cctacggggc ccgcaccctc
     3661 ctggactcgg tgaacctcac cgtggccacg ggcacccgca tggccctggt gggacccaac
     3721 ggcagcggca agacgaccct gatgcggatc atggcatcgc aggtccgtgc ggacatcggg
     3781 tccgtggtcg tcgagcgcgg gacccgggtc tcctacgtgc cccagtcggg ggtcgtccac
     3841 gcgggctcat ccctgcgcga agaggcggag aaggccttct cctacggggc cggcatcgag
     3901 gccgagctgg cggctctcca ggagcgcctg gggacgatat ctcagggaga cccgcaggcg
     3961 gaggggctcc tgtggaagca ccacgagctg caggagaggc tggaggcgag cggataccac
     4021 cgacgggacg aggccatcca gcgaatcctg agtggcctcg gcttctccct ggaagatctg
     4081 gagaaaccca gctcttcgtt ctcggcggga tggcagatgc ggatcgctct ggctcgcgcc
     4141 atcctggaga ctccggacat cctcctgctc gatgagccca ccaactacct ggacctggag
     4201 gctcgagact ggctggaggg gcacctggcc gcgttccccg gcgggctgct cctggtttcc
     4261 cacgaccgct acttcctgga cgtcgtggtg accgcggtgg cggagatcta catggcccgg
     4321 gtctccgtgg tcacgggcaa ctacacccat tacgaagagc gccgggccca ggagctcgcc
     4381 gccatcgtcg agcgttggaa gcagcagcag gaggagatcg cgcgggccga gtcgttcatc
     4441 acccggttcc gctacaaggc ctccaaggcc cggcaggtgc agagccgcat cacggcgctg
     4501 gagaagatcg agcgcatcga gatcccgccc gtggtcaaag gcatccactt caccttcccc
     4561 aagcctcccc acagcggcca gatcgtgctc acggccacgg gccttgccaa gtcctacgga
     4621 ggccggacgg tctttcgcgg cgtggacgtg gaggtgaccc ggggagagaa gctggtggtg
     4681 gtgggggtga acggggccgg gaagtcgacg ctccttcggc tcatctccgg ccgcgaggct
     4741 ccggacgagg ggtcgttgag ggcaggcacg ggggtggtgc cggcgtacta ctcacaggag
     4801 aacg
//
LOCUS       DASRZS010000691         7561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_4614329, whole genome shotgun sequence.
ACCESSION   DASRZS010000691 DASRZS010000000
VERSION     DASRZS010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4614329"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VFH83_16855"
     CDS             complement(<1..1081)
                     /locus_tag="VFH83_16855"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6488100.1"
                     /translation="MSTWTKRLVVLAVVVLCAAGLWAGGTAEKAAVALTWYYPGNYPQ
                     KDQDMVFNQFNTLLKQKMNTTIDFKPVAWGDYDQKMQAIIGSGEKFDLCYTANWINNF
                     WQNVAKGAFLPLDDLLAKDAPKLKAMIPDKIWNAAKVNGKLYGIINYQISAYTSGLMF
                     PKSLVTKYNFDVTKVKKLEDLEPYLKAVLGGEPGLTAVLGVANTPGTTVGFVNAYLGM
                     EEIGGRVVPGVILNNDPALKVVNQFKTDQMKAWLKLERDWYQKGYIMKDALAVKDLNP
                     NLSTGKVGVSFVGTYKPGGDAEDQARLGYPVVEIPISPSYLQTGSCVATMHAISITSG
                     SPDRAMQFMELMNTDVPMYNLLTWGI"
     gene            complement(1183..2073)
                     /locus_tag="VFH83_16860"
     CDS             complement(1183..2073)
                     /locus_tag="VFH83_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018756859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6488101.1"
                     /translation="MIESRRTKALSRALIHTVMILFSLICVVPLIAVVSVSLSSELSI
                     KLSGYSLLPVHFDAAAYRYIFRSPWGIVHSYGITFAMTATGTFLSLLVISLLAFPLSR
                     PDYRYRNKVSFFVFFTMLFNGGLVPWYILIVRYLQLKNHFLVLVLPYLVVPWFVLLLR
                     TFFKQLPMSLFESAKIDGASEFRSFFQILLPLSKPALATIGLFICLNYWNDWWLALLY
                     IDEERLVPLQYMLYRMMSNITFLTSQMSTTAVTIDLSTFPNESARMAMCVLAAGPMLF
                     IFPFFQKYFVRGLTAGSLKG"
     gene            complement(2085..3041)
                     /locus_tag="VFH83_16865"
     CDS             complement(2085..3041)
                     /locus_tag="VFH83_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6488102.1"
                     /translation="MPAVLRGGEPTLREKRGPRRYFRANLEFTVMALPAMVLLLIFNY
                     LPMAGIIIAFKRFRVNLGFFRSPWVGLQNFSFFFQSQDAWRITRNTLGYNALFIILGL
                     AISVTFALLLFELTSRAILKLYQTVFFFPYFLSWVVVGYVLYAFLSTDYGLANGLLRA
                     LGKQPITWYIVPGYWPPILVFMNLWKTVGYFSVIYYAGLMGIDRELYEACVIDGANKW
                     QTATRISVPLLSHLMVALVILQIGRIFYADFGLFYNLPRDIGTLYPATDVIDTYVFRS
                     LRVTGDIGMSSAANFYQAIVGFLLVLISNGVVRRLSPEHSLF"
     gene            complement(3026..4195)
                     /locus_tag="VFH83_16870"
     CDS             complement(3026..4195)
                     /locus_tag="VFH83_16870"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET6488103.1"
                     /translation="MTSHERVRRLLSGAAIDQVPVEDLPWDDTVLAWAAQGYPLRRTW
                     REQGESTWRPSDGRWVASERSEWRDEPVPPWEHFHWDMALIWLSFDKLPMIGMSELLE
                     ETSEWEVRRNGAGAALKYWKHHNGTPEHIDFRMTSREIWERDYRPLLERFDPSRLDVA
                     KTRKELEAGKARGAWTFYGDLFVWELARGSMGDIALYESVLLDPDWILDYNRVYTDFY
                     KRYFTYLFETAGVPSGIWLYEDLGYRNGLFVRPEVLGRLYFPFYREIVDFFHARDLPV
                     VLHSCGSVPQALPMIVEAGFDGLNPMERKAVGNEPFDFAERYGQRIAFVGGLDARILE
                     TNDLPLIRREVAFYMEGMKSRGARFVFGTDHSISPRVTYQSYRTALEVYRECRPY"
     gene            4325..6748
                     /locus_tag="VFH83_16875"
     CDS             4325..6748
                     /locus_tag="VFH83_16875"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET6488104.1"
                     /translation="MPSPRETWRYGSLFLRILVTLGLLLVATIVALAFALSASYERTT
                     RRILEQSQLTLLSQVTYGFNSLDTQARAFATSLSVDNRVIPLLYGADLDIWSIVKSLD
                     AISVRVQSTSFVDSVYIYNRPTGSFYTTMGQGIRTVSDFFDPQISSMVEAGRAVPKLG
                     PIPRRIERAVLQSATPQEVNLYTYVYYASWPESGFPSAIFVNVPAQSLMANLDSLSQQ
                     QRGQGGSIVVVAGDGRIMADASPERFLRDASGDRIVSAVLRASGRSGSFMAPNSPGTG
                     PSVVSWIASGPPGWRFIQVTPYQAILRPLIGQRSLILGISIAFAAAALLVAFFASRLL
                     YLPIGSLVRHARRVLDSSEAIGRSENEMRFLSEAFSHTAGMVTLLRRFKADNLKTLRD
                     DLLRGVLTGSLSAEEAMARLADFGLRLAPHGFFQVAACRLDRGMAGSGTRPASGDGVP
                     AGNADGIEVRTFILERMARALTGVRFDCETVETERDLVAMVFSFSDPDAAFDGVEGHV
                     RGLQRYLADGIGASLSAGLSDAGRGLASLSALYAQAVELASYRMVFGRGCLVTSARVA
                     ESSGTPYRFPVEGETRILEALTRCDLSQAEAALRETLDGLRRQDYETIRLSITRVASS
                     LFDHLTMMESNGAVRFPFRFSHFMRSLDRCETLPEVQAEFLGLLSDVEEVLRSAAAHK
                     PERNVQAVRDLIEERYRDCTLNVAALAEQMRMSPGYLARIFRERSGRSIAEYLTEVRL
                     RKARELLGEGACAINEVSQRVGIANRRFLITKFKESFGVTPSEYRRTAGVSKTATLPR
                     RPTARTGPA"
     gene            6780..>7561
                     /locus_tag="VFH83_16880"
     CDS             6780..>7561
                     /locus_tag="VFH83_16880"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6488105.1"
                     /translation="MKRTVSLLLAAALGVLFTSPVSAQSYRVLTYNLGLLRVLGSDYV
                     PNVQGRALVAPRELAAFAQANSPQIILLEEVWNDQNAKAIQKELAPQGYTFVRPAQAS
                     LLGLSGGLLLAVKSPLVVVDWKFTRFSKSTFMDGMAHKGVLEATIQDPSNGDQQFAVV
                     GVHTLAMETVKGEVKDQGQIDAFAVQAKEILAGVDRRSDSGRLPTILLGDFNVGPGYA
                     DAQYRKIADSGKLVEVGASLTPDKPIITWDPQNPLVKYGEFP"
BASE COUNT         1359 a         2394 c         2472 g         1336 t
ORIGIN      
        1 cgatgcccca ggtgagcagg ttgtacatcg gaacgtcggt attcatgagc tccatgaact
       61 gcatggcccg gtccgggctc ccggaggtga tgctgatggc gtgcatggtg gccacgcagg
      121 agccggtctg aaggtacgaa ggggaaatgg ggatctccac gacggggtag cccaggcgcg
      181 cctggtcctc cgcgtccccg cccggcttgt aggtccccac gaaggagacg ccgaccttgc
      241 cggtggagag gttcgggttg aggtccttca ccgccagggc gtccttcatg atgtagccct
      301 tctggtacca gtcccgctcc agcttgagcc acgccttcat ctggtccgtc ttgaactggt
      361 tgaccacctt gagcgccgga tcgttgttca ggatgacccc cggaaccacg cgaccgccga
      421 tctcttccat gccgagataa gcgttcacga atccgacggt ggtgcccggc gtgttggcca
      481 ctcccaggac ggcggtgagc ccgggctcgc cgcccaggac ggccttgaga tacggctcga
      541 ggtcttcgag cttcttcacc ttggtgacgt cgaagttgta cttggtcacc agggacttgg
      601 ggaacatgag gccggaggtg taggcggaga tctggtagtt gatgatgccg tacagcttgc
      661 cgttcacctt cgccgcgttc cagatcttgt ccggaatcat ggccttgagc ttgggggcgt
      721 ccttggccag gaggtcatcc agcgggagga acgcgccctt ggccacgttc tgccagaagt
      781 tgttgatcca gttggccgta tagcagagat cgaacttctc cccggagccg atgatcgcct
      841 gcatcttctg gtcatagtcg ccccatgcca cgggcttgaa gtcgatggtg gtgttcatct
      901 tctgcttgag cagggtgttg aactggttga acaccatgtc ctggtccttc tgcggatagt
      961 tgcccgggta gtaccaggtc agggccaccg ccgccttctc cgcggtgccc ccggcccaca
     1021 ggcctgccgc gcacaggacg accacggcca gcacgacaag cctcttggtc cacgtgctca
     1081 tacgagccct ccttcaggaa aactctccag cgggtcgacg acccgcaagc tctcaaacgc
     1141 aagcgcggag cccccggcgg cgcagcggcg gcaccccatg ccctatcctt tgagggagcc
     1201 cgccgtgagc ccccgtacga agtacttctg gaagaacgga aagatgaaga gcatcggacc
     1261 cgccgccagc acgcacatgg ccatgcgcgc cgattcgttg gggaacgtgc tcaggtcgat
     1321 ggtcaccgcc gtggtggaca tctgggaggt gaggaaggtg atgttggaca tcatccggta
     1381 gagcatgtac tgcaagggga ccagccgctc ctcgtcgatg tacagcaggg ccagccacca
     1441 gtcgttccag tagttcaggc agatgaaaag accgatggtg gcgagcgccg gcttggaaag
     1501 cgggagcagg atctggaaga agctgcggaa ctcgctggcg ccgtcgatct tggcggactc
     1561 gaacagggac atgggaagct gcttgaagaa ggtccgcagg aggagcacga accaggggac
     1621 cacgaggtag ggcaggacca agaccaggaa gtgattcttg agctgcagat agcggacgat
     1681 caggatgtac cacggcacca gcccgccgtt gaagagcatc gtgaagaaca cgaagaagct
     1741 caccttgttg cgatagcggt agtccgggcg cgacaagggg aacgccagga gggagatcac
     1801 gagcaaggag aggaaggtgc cggtggcggt catggcgaag gtgatcccgt aggaatggac
     1861 gatcccccag gggcttcgga agatgtagcg gtaggccgcg gcgtcgaagt gcaccgggag
     1921 gagcgagtag ccgctgagct tgatcgagag ctcgctggac agagaaaccg agacgaccgc
     1981 gatcaacggc acgacgcaga tcaggctgaa gaggatcatc accgtgtgga tgagcgccct
     2041 cgacagggcc ttggttctgc gggactcgat cacgcccctt cctcctagaa gagcgaatgc
     2101 tcggggctga gccggcggac taccccgttg gagatcagga ccaggaggaa ccccacgatg
     2161 gcctggtaga agttggccgc ggaggacatg ccgatgtccc cggtgacgcg cagggaccgg
     2221 aacacgtacg tgtcgatcac gtccgtggcc gggtagagcg tgccgatatc ccgtggcagg
     2281 ttgtagaaga gaccgaaatc ggcgtagaag atgcggccaa tctgcaggat caccagcgcc
     2341 accatgaggt gggagaggag gggcacggag atccgcgtcg ccgtttgcca cttgttggcg
     2401 ccgtcgatga cgcaagcctc gtagagctcg cggtcgatcc ccatgagccc cgcgtagtag
     2461 atgacggaga agtagcccac cgtcttccag aggttcatga acaccaggat gggcggccag
     2521 tatccgggga cgatgtacca ggtgatgggc tgctttccca atgcgcggag cagaccgttg
     2581 gccagcccat agtccgtgct cagaaatgcg tacagcacgt agccgaccac gacccaggag
     2641 aggaagtaag ggaagaagaa cacggtctgg tagagcttga ggattgcgcg gctggtcagc
     2701 tcgaagagca gcagcgcgaa ggtcacggag atcgccaggc ccaggatgat gaacagggcg
     2761 ttgtagccca gcgtattgcg ggtgatccgc caggcgtcct gggactggaa gaagaagctg
     2821 aagttctgaa ggcccaccca cgggcttcgg aagaagccca ggttcactcg aaagcgcttg
     2881 aacgcgatga tgatcccggc catgggcagg tagttgaaga tcaggagaag caccatggcg
     2941 ggcagggcca tcaccgtgaa ctcgaggttt gcccggaagt atcttcgggg tcccctcttc
     3001 tcgcgcagcg tcggctctcc gcctctcaat acggccggca ttcccggtag acctccagcg
     3061 ccgtccgata gctctggtac gttacgcgcg gcgagatgga gtggtccgtt ccgaagacga
     3121 accgcgctcc cctggatttc attccttcca tatagaaggc tacctcccgg cggatgaggg
     3181 gaagatcgtt cgtctccagg atccgagcat ccagtccgcc gacgaaggcg atgcgctgtc
     3241 cgtagcgctc cgcgaagtcg aacggctcgt tgcccaccgc cttgcgctcc atggggttca
     3301 ggccgtcgaa gcccgcctcg acgatcatgg gcaaggcctg gggcacgctc ccgcaggaat
     3361 gcagcaccac cggcaggtcg cgggcgtgga agaagtccac gatctcccgg tagaacggga
     3421 agtacagcct tcccaggacc tcgggtcgaa cgaagaggcc gttgcgatat cccaggtcct
     3481 cgtagagcca gatgccgctg ggcacaccgg ctgtctcgaa aaggtacgtg aagtagcgct
     3541 tgtagaaatc cgtgtagacc cggttgtagt cgaggatcca gtcggggtcc aggagcacgc
     3601 tctcgtacag cgcgatgtcc cccatgctcc ctcgggccag ctcccagacg aaaaggtcac
     3661 cgtagaaggt ccaggcccct cgggccttcc ccgcctcaag ctccttgcgg gtcttggcga
     3721 cgtccaggcg gctcgggtcg aatcgctcca ggagcgggcg gtagtcccgc tcccagatct
     3781 cgcgcgaggt catccgaaag tcgatgtgct ccggggtccc gttgtgatgc ttccagtact
     3841 tcagcgccgc gcctgcgccg tttcgccgga cttcccactc cgaggtctcc tccagcagct
     3901 cgctcatccc gatcatggga agcttgtcga aggagagcca gatcagggcc atgtcccagt
     3961 ggaagtgctc ccaagggggg accggctcgt cgcgccactc cgaccgctcc gacgccaccc
     4021 accgcccgtc gctgggccgc caggtggatt cgccctgctc cctccaggtc cgccgcagcg
     4081 ggtagccctg agcggcccac gccagcaccg tatcgtccca cggcaggtct tccacgggga
     4141 cctgatcgat cgcggcgccg gagagcaggc ggcgcactct ctcgtgggag gtcatgcgct
     4201 actctcctcc cggttgctcg cgctgtaaag tgtctattcc ggaacaaatg tccgattccg
     4261 gaacctttcc cgcctcgggt ctttcttgac acctgcccgg gggcctcata cgatggatgg
     4321 ccgaatgccg tcgccgcgtg agacgtggag atatggaagc ctcttcctcc gcatcctggt
     4381 gacgctgggc cttctgctcg tggccacgat cgtggccctg gcgttcgcgc tgagcgccag
     4441 ctatgagcgc accacccggc ggatcctgga gcagtcccag ctcacgctgc tctcccaggt
     4501 gacctacggc ttcaacagcc tggacaccca ggcgcgcgcg ttcgccacct ccctttccgt
     4561 ggacaaccgg gtcatccccc ttctgtacgg cgcggacctc gacatctggt ccatcgtcaa
     4621 gagcctggat gcaatcagcg tccgggtgca gagcacgtcg ttcgtggact ccgtgtacat
     4681 ctacaaccgc ccgaccggca gcttctatac caccatgggc caggggatcc gaacggtatc
     4741 ggacttcttc gatccccaga tctcctccat ggtggaggcc ggccgggcgg tgccaaagct
     4801 gggtcccatc ccacggcgca tcgagcgcgc ggtgctccag tccgccaccc cgcaagaggt
     4861 gaacctctac acgtacgtct actatgcgag ctggccggag tcgggttttc cctccgccat
     4921 cttcgtgaac gtccccgctc agagcctgat ggccaacctg gactccctga gccagcagca
     4981 gcgaggtcaa ggcggcagca tcgttgtggt ggccggcgac ggccggatca tggcggacgc
     5041 gagcccggag cggttcctcc gcgacgcatc gggggatcgg atcgtgtccg cggtcctgcg
     5101 cgcatcgggg cgatccggga gcttcatggc ccccaactcc ccgggaacgg gaccttccgt
     5161 ggtctcctgg atcgcctccg ggccgccggg gtggcgcttc atccaggtga cgccctacca
     5221 ggccatcctc aggccgctga tcggacagcg gtccctcatc ctgggaatct ccattgcgtt
     5281 cgcggccgcg gcgcttctcg tggcattctt cgcgtcccgg ctcctctacc tgcccatcgg
     5341 ctccctggtc cgccacgctc ggcgcgtgct cgattccagc gaagccatcg gccgatccga
     5401 aaacgagatg cggttcctgt cggaggcttt ctcccatacg gccggcatgg tgacgctcct
     5461 gcgccggttc aaggcggaca acctcaagac actccgggac gatctccttc gaggggtcct
     5521 gaccggctcc ctttccgcgg aggaggcgat ggcccggctg gcggacttcg gtctgcgcct
     5581 ggcgccccac ggtttcttcc aggtggcagc ctgtcggctg gaccggggaa tggccggctc
     5641 gggaacgcgt cccgcttcgg gcgatggggt ccccgccggc aacgcagacg gcatcgaggt
     5701 gaggacgttc atcctggagc gcatggcacg cgcgctcacc ggcgtgcgct tcgattgcga
     5761 gaccgtcgag accgagcggg acctggtggc catggtcttc tccttctccg acccggacgc
     5821 ggcgtttgac ggcgtcgaag gacacgtgcg tggcctccag cgatacctgg cggatggcat
     5881 aggcgcgtcc ctttccgccg gcctgagcga cgccggccgg gggctcgcgt ccttgagcgc
     5941 gctgtacgcc caggcggtgg agttggcctc ctatcgcatg gtgttcggtc ggggttgcct
     6001 ggtgacttcg gcccgggtgg cggagagctc cgggacgccc taccggttcc ccgttgaggg
     6061 agagacccgg atcctggaag cgctgacccg ttgcgatctg tcccaggccg aggccgccct
     6121 gcgggagacc ctggatggcc ttcggcgcca ggactacgag accatccggc tctcgatcac
     6181 gcgcgtggcc tcgtccctct tcgatcatct gacgatgatg gagtccaacg gcgccgtccg
     6241 gttcccgttc cgattcagcc acttcatgag gagcctcgat cggtgtgaga cgctgcccga
     6301 ggtgcaggcc gagttcctgg gcctcctttc cgacgtcgag gaagtgctgc gcagcgcggc
     6361 cgcgcacaag cccgaacgaa acgtccaggc ggttcgggac ctgatcgaag aacgctaccg
     6421 ggactgcacg ctgaacgtcg ccgccctggc cgagcagatg cgcatgtccc ccggatacct
     6481 ggcccggatc ttccgggagc gaagcggaag atccatcgcg gaatacctga cggaggtgcg
     6541 gttgcgcaag gcgcgggagc ttctggggga gggagcctgc gccatcaacg aggtatcgca
     6601 gcgggtcggg atcgcaaacc gtcggtttct catcacgaag ttcaaggaga gcttcggggt
     6661 aaccccgtcg gagtaccgtc gaacggccgg tgtatcgaaa acggccacgc ttccccggcg
     6721 ccccaccgcg cgtacgggac cggcttgacg ggccggtcct gggcgtgcac tttgtctgca
     6781 tgaagcgaac ggtgtccctt ctgctggccg cggccctggg cgtgctcttc acgtcgccgg
     6841 tatccgctca gagctatcgc gtgctgacct acaacctcgg cctgctgcgc gtcctgggat
     6901 cggattatgt gcccaacgtc caggggagag cgctggtggc tcctcgagag ctcgccgcct
     6961 tcgcgcaggc caattccccc cagatcatcc tgctggagga ggtctggaac gaccagaacg
     7021 ctaaagccat ccagaaggag ctggctcccc agggctacac gttcgtgagg ccggcccagg
     7081 cctcgcttct ggggctgagc ggcggcctgc tcctggcggt aaagtctcct ctggtggtcg
     7141 tggactggaa gttcacgcga ttcagcaaga gcaccttcat ggacggcatg gcgcacaagg
     7201 gagtcctcga ggcgaccatc caggatccgt ccaacgggga ccagcagttc gccgtggtgg
     7261 gcgtgcacac gctggccatg gaaaccgtga aaggggaggt caaggaccag ggacagatcg
     7321 acgccttcgc cgttcaggcc aaggagatcc tggccggcgt ggaccgccgt tcggactccg
     7381 gtcggctgcc caccatcctg ctgggggact tcaacgtcgg acccgggtac gcggacgcgc
     7441 agtaccgcaa gatcgccgac tccggcaagc tcgtcgaggt tggcgccagt ctgactccgg
     7501 acaagccgat catcacctgg gacccccaga acccgctcgt gaagtacgga gagttcccga
     7561 a
//
LOCUS       DASRZS010000692         8141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate SMAG_U14774
            k141_3461273, whole genome shotgun sequence.
ACCESSION   DASRZS010000692 DASRZS010000000
VERSION     DASRZS010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970316
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (soil metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,384
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3461273"
                     /isolate="SMAG_U14774"
                     /isolation_source="soil"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VFH83_16885"
     CDS             <1..494
                     /locus_tag="VFH83_16885"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET6488106.1"
                     /translation="REMDPANVDASDLPLDPISSLHVTGAPMDVDPASWRLSLSGAGL
                     TSPTALTYQDLQGMPTVARRAILICPGVFADYAEWEGVPLDAVLAKAGLSASWSKVSF
                     VAMDGYREEFTRGEVQTHLLFLALKVNGQVLPRDHGFPIRLVAVDLYGGRWVKWIKEI
                     RVE"
     gene            514..1971
                     /locus_tag="VFH83_16890"
     CDS             514..1971
                     /locus_tag="VFH83_16890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6488107.1"
                     /translation="MLRTFYGRLSTIFLLLILGLGAGWVAIAIVSAGHLFDDVEQLLN
                     RGYAQSIASELSPLVASGFRGDRVTSAIHYMMVLNPMVSIYLLDSQGRVSASFTNPAE
                     PLARSGVNLSPVLKFISSGGREVLKGDDPLSATRSRPFSAAPLMLGGDQGFVYIILGG
                     ARYDAALRMISQSFYLRAGLAAFLLALASTLVLGFSLFFLVTRRLRSLSESMAAFQQG
                     ELSRRLDVGARDELGTLARSFNQMAETIAADMDKLKGAERMRRELVENISHDLRSPLT
                     SLQGYLETILRKDPDLEPAQRREFLEISLRNASSLRRLVEDLFELARLDARQILPRRE
                     PFQVAELAQDVVLKLAPQARSAGVTVTTEPGPDLPLVWGDIALIERVLTNLLENAISF
                     TPASGSTRVEMSRQGSGVRIAVCDTGPGIPAEELGRIFERFYRTDASRSRSSGGAGLG
                     LAIARQIVELHGGALEVTSRPGQGSSFSFRLEQAG"
     gene            complement(2024..2812)
                     /locus_tag="VFH83_16895"
     CDS             complement(2024..2812)
                     /locus_tag="VFH83_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6488108.1"
                     /translation="MLTRAQVAFYQEHGYLHIPQVFTPSEVQELSDELDKLVREWAFT
                     DQGWTGPWRRAYMDEQTEKKSKLTGMHDLQFYSAAWMRAVTNKKLAEAMGDLLGPNVE
                     LHHTTLHIKPPETGHPFPMHQDHPFYIHATGAFVDVLVHLDDTNDQNGEIRFLDGSHK
                     NGPLVHVTQTADGPCSPHLPTDRWRLEDTVAVPAKRGDVVCFSIHTVHGSYINRTDKP
                     RRLVRVGYRDPQNLQTAGQSSGRPSIMVLGYRDRRDGQALLKTE"
     gene            2978..4072
                     /locus_tag="VFH83_16900"
     CDS             2978..4072
                     /locus_tag="VFH83_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013917263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6488109.1"
                     /translation="MEKTGIGVIGCGNISDIYLKNLQQTFLGTRVVACADILPERAKA
                     KAEKWSVPKAVSVDELLRDPEVEIVVNLTIPVVHAEVDRKALEAGKHVHSEKPLAVTF
                     ADGSALMKLAQKRSLRLGCAPDTFMGAGIQACRALIDDGAIGAPVGAVAFMTCPGHES
                     WHPDPEFYYKPGGGPMLDMGPYYVTALVSLLGPVRRVSGASRISFPERTIGSEPKRGT
                     KIKVEVPTHVSGTLEFADGAIATLIMSFDVWGAHLPWIEIYGSEGSLAVPDPNTFGGP
                     ISLLPARSKEWKSMPVTRAYSENSRGVAVADMAKAIREKKQHRANGELAGHVLEIMHG
                     ILEAAKGAKRYDLTTTCERPKPVPADGGGL"
     gene            complement(4082..6046)
                     /gene="acs"
                     /locus_tag="VFH83_16905"
     CDS             complement(4082..6046)
                     /gene="acs"
                     /locus_tag="VFH83_16905"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET6488110.1"
                     /translation="MAEKTTAIDDLLREEREFTPPSDFSSHAQVRDARIYPDAARDPV
                     AWWAQEASRLQWSRPWDKAMEWDPPHARWFVGGQLNITVNCIDRHLNGPRRNKAALIW
                     EGEDGEVRTLTYLQLHREVCKFANCLKALGVQKGDRVAIYMPMLLESAVAMLACARIG
                     AVHNVVFGGFSPEALADRIIDSQAKVLITADGGWRRGNVVQLKHNADRALERCPSVQH
                     VVIVKRLPGEPHLLHIMEGRDHFYHRIMDHQKVECPAIPVDAEDNLFILYTSGTTGKP
                     KGIVHTVGGYAVGVQTTTRMVFDLRDEDVFWCTADIGWVTGHSYVVYGPLLCGATVLM
                     YEGAPDFPERDRTWQLIEEHQVSVLYTAPTAIRTFMKWGKELPGKHNLTSLRLLGSVG
                     EPINPEAWMWYHETIGGGRCPVVDTWWQTETGAIMITTLPGISTMKPGFVGTPLPGIM
                     ATVLDESGKEVKTGSGLLAITHPWPSMLRTVWGDDDRFRSTYFKKWSDKLYFPGDGAK
                     RDAKGNYMILGRVDDVLNVSAHRIGTMEVESALVDHPAVAEAAVVGRNHDIKGQAIVA
                     FVSLKGGQKPSPDLVDELKEHVVTKIGALARPEEVLFAHDLPKTRSGKIMRRLLRDIA
                     EGRALGDVTTLADPAVVASLREDYEEKFGT"
     gene            6166..7098
                     /locus_tag="VFH83_16910"
     CDS             6166..7098
                     /locus_tag="VFH83_16910"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET6488111.1"
                     /translation="MMVRRTMLAAGLLAIGSLAAGAVIGGRSSAWAQAMGTFPPTTTE
                     STGERELGAFVQAYPDRLIGPELRDGDWAVKLGSRWFAWAHGRILPEDQRSAWESYAA
                     YRFYRYPVGALPPLPQLDAQSIARLQAIVKQSQARPPRRSEAFLESLLGAATLDQIRG
                     NLVTVPLLGFPVQIHSVAAAALRKVAAECALLVKTDPEAAAFFAGIHEIDGFNYREVA
                     GTATRSYHGYGLALDLIPRSYGGKPVYWLWAMQAGESWWAIPYDRRWMVPLSVVTAFE
                     NNGFVWGGKWMFFDMMHFEYRPELLSLAREGAAP"
     gene            7202..7369
                     /locus_tag="VFH83_16915"
     CDS             7202..7369
                     /locus_tag="VFH83_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6488112.1"
                     /translation="MADEPKAARKGPRRRRRKGAARKTVAKRGQKITLSIPDLNKLTK
                     AEIQFIATYKA"
     gene            complement(7415..>8141)
                     /locus_tag="VFH83_16920"
     CDS             complement(7415..>8141)
                     /locus_tag="VFH83_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6488113.1"
                     /translation="SRERARTLFDGMIRRGLKKRWWMQTSINSAEDEGLLRLAGRSGC
                     LFALIGFESIAEDQLKDMKKGINLRIGVDNYRRVIRSFHRAGIGVVGSFIIGNDHESV
                     RYYRQIARFLVSAGVDAVQLAILTPFPGSMLMERMKAEGRLTRTSFPQDWASYRLSYV
                     VRSPVGVDAETIYRGDNYIKHHIYSFPRSQWRSLRSFVGIRNLVSYTAVHRFDRALKR
                     SWKGAHYYGKYSNRLEEGARPQG"
BASE COUNT         1301 a         2670 c         2729 g         1441 t
ORIGIN      
        1 tccgagagat ggaccccgcg aacgtggacg ccagcgacct gccgctggat ccgatctcct
       61 cgctccacgt gaccggagcg cccatggacg tggacccggc gagctggcgg ctgtcgctct
      121 ccggcgccgg actcacctcc ccgacggcgc tcacctatca ggatcttcag ggcatgccga
      181 ccgtggcccg gcgcgccatt ctgatctgcc ccggggtgtt cgcggactac gccgagtggg
      241 agggagtgcc gttggacgcg gtcctggcca aagccggcct ctccgcgtcc tggtccaagg
      301 tcagcttcgt ggcgatggac ggctaccggg aggagttcac gcggggcgag gtccagaccc
      361 acctcctctt tctggctctc aaggtgaacg ggcaggttct gccccgggat cacggattcc
      421 cgatccggct cgtggcggtg gacctgtatg gcggccgctg ggtgaagtgg atcaaggaga
      481 tccgggtgga atagagtata ttctcacccg gtcatgcttc ggaccttcta cggacggctc
      541 tccacgatct ttctcctctt gatcctgggt ctgggggccg gctgggtggc catcgccatc
      601 gtatccgccg ggcacctgtt cgacgacgtc gaacagcttc tcaaccgggg gtatgcccag
      661 agcatcgcct ccgagctgtc tcccctggtg gcatcggggt tccgcgggga ccgggtcacc
      721 agcgccatcc actacatgat ggtgctcaac cccatggtgt ccatctatct cctggactcc
      781 caggggcgcg tcagcgcctc cttcacgaat cccgcggagc cgctcgcccg ctcgggcgtg
      841 aacctctctc cggtgctgaa gttcatctcc agcggcggcc gcgaagtgct gaagggggat
      901 gacccgctta gcgccacgcg ctcgcgcccc ttctccgcgg cgccgctcat gctgggaggc
      961 gaccagggat tcgtctacat catcctgggc ggcgcgcgct acgacgcggc gctgcggatg
     1021 atcagccaga gcttctacct gcgcgcgggc ctggccgcgt ttctccttgc gctggcctcc
     1081 acgctggtgc tcgggttctc cctgtttttc ctggtcacgc ggaggctgcg ctccctgagc
     1141 gaaagcatgg ccgcgttcca gcagggcgag ctttcccgac gcctggatgt cggagccagg
     1201 gacgagctgg gcaccctggc ccgttcgttc aaccagatgg ccgagacgat cgccgcggac
     1261 atggacaagc tcaagggcgc cgagcgcatg cggcgggagc tggtggagaa catttcccac
     1321 gaccttcgaa gccccctgac ctcgctccag gggtacctgg agaccatcct aaggaaggat
     1381 ccggacctgg aacccgccca acggcgagag ttcctggaga tcagcctgcg caatgcctcc
     1441 agcctgcgtc ggttggtgga agacctcttc gagctggccc ggctggatgc gcgccagatc
     1501 ctcccccggc gcgaaccgtt ccaggttgcc gagctggccc aggacgtggt gctgaagctc
     1561 gcgccccagg cccggtccgc gggcgtgacg gtcaccacgg agcctgggcc cgatctgccg
     1621 ctggtctggg gggacatcgc cctgatcgag cgagtgctga ccaacctcct ggagaacgcc
     1681 atctccttca cgccggccag cggctcgaca cgggtcgaga tgtcacggca ggggtcgggt
     1741 gtcaggatcg cggtatgcga caccggtccc ggcatccccg ctgaggagct gggcaggatc
     1801 ttcgagcgat tctatcgtac ggacgcgagc cgcagccgtt cctcaggcgg agccgggctg
     1861 ggcctggcca tcgcgcggca gatcgtcgaa ctgcatggag gcgcgctgga ggtaaccagc
     1921 cgaccgggtc aggggtcgag cttctcgttc cgcctggagc aggccggcta agcggccgtg
     1981 tcagggccgg gccggcgaaa gcgccgggac ccgcggatcg cccttactcc gtcttcagga
     2041 gagcctggcc gtcccgtcga tcgcgatacc ccaggaccat gatgctgggg cgccccgagc
     2101 tctgcccggc cgtctgcagg ttctgagggt cgcggtagcc cacgcggacc agccgacgcg
     2161 gcttgtcggt ccggttgata taggacccgt gcaccgtgtg gatgctgaag cacaccacgt
     2221 ctccgcgctt ggccggcacg gccaccgtat cttccagccg ccagcggtcc gtaggcaggt
     2281 ggggagagca gggcccatcc gccgtctgcg tcacatggac cagcggcccg ttcttgtggg
     2341 agccatcgag gaaccggatc tcgccgttct ggtcgttggt gtcatccagg tggaccagca
     2401 catcgacgaa ggcgccggtg gcgtggatgt agaagggatg atcctggtgc atggggaagg
     2461 gatggccggt ctcgggcggc ttgatgtgca gcgtggtatg gtgcagctcc acgttggggc
     2521 ccaggaggtc ccccatggcc tccgccagct tcttgttggt cacggcgcgc atccaggcgg
     2581 cggagtagaa ctgaaggtcg tgcattccgg tcagcttgga tttcttctcg gtctgctcgt
     2641 ccatgtacgc ccggcgccac gggccggtcc acccttggtc cgtgaaggcc cattcgcgga
     2701 ccagcttgtc cagctcgtcg gaaagctcct ggacctcgga cggtgtgaat acctggggaa
     2761 tatggagata cccgtgctcc tggtagaagg ctacctgggc tcgcgtcagc atgctgtgcc
     2821 tccgggaccg cggcggaagc cacggcctgg gggagaagat acccaaaccg ggcagagacc
     2881 gcccaacggc ccctgcctgc catgctctgc ttgcagcgca gggcgccccg catcatactg
     2941 atcccacaca ccgctcagga ggaaagggag gaaagggatg gagaagaccg gtatcggcgt
     3001 cattgggtgc ggcaacatca gcgatatcta tctcaagaac cttcagcaga cgttcttggg
     3061 gacccgcgtg gtggcctgcg cggacatcct gccggagcgc gccaaggcca aggcggagaa
     3121 gtggtcggtc cccaaggccg tgagcgtgga cgagctgctg cgggaccccg aggtggagat
     3181 cgtggtgaac ctgaccatcc ccgtggtgca cgcggaggtg gacagaaagg cgctggaggc
     3241 cggcaagcac gtccacagcg agaagcccct ggccgtaacc tttgcggacg gctcggcgct
     3301 catgaagctg gctcagaagc ggtcgctgcg cctgggatgc gccccggata ccttcatggg
     3361 cgcgggcatc caggcgtgtc gagccctcat cgacgacggc gccattggcg cgccggtggg
     3421 cgccgtggcg ttcatgacct gcccggggca cgagagctgg caccccgacc ccgagttcta
     3481 ctacaagccc ggcggcggcc caatgctgga tatgggtccg tactacgtca ccgcgctggt
     3541 gtccctcctg ggacccgtcc gccgcgtgag cggcgcctca cggatcagct tccccgagcg
     3601 caccatcggg agcgagccca agcgcggcac caagatcaag gtggaggtcc cgacccacgt
     3661 ctcggggacg ctggagttcg ccgatggcgc catcgccacc ctcatcatga gcttcgacgt
     3721 ctggggcgcg cacctgccct ggatcgagat ctacggaagc gaaggatccc tggcggtgcc
     3781 ggatccgaac acctttggcg gacccatctc tctgctgccc gcgcggagca aggagtggaa
     3841 gtccatgccc gtgacgcgcg cctactcgga gaactcccga ggcgtggcgg tggccgacat
     3901 ggccaaggcc atacgggaga agaagcagca ccgcgccaac ggcgagctgg cgggtcacgt
     3961 gctggagatc atgcacggga tcctggaagc cgcaaaaggc gcgaagcggt acgacctgac
     4021 gaccacctgc gaacgcccca agcccgtgcc cgcggacggc ggagggctgt agagcccgct
     4081 ctcaggtccc gaacttctcc tcgtagtcct cacgcaggga ggcgaccacg gccgggtccg
     4141 ccagcgttgt cacgtctccc agcgcccgtc cctcggcgat atcccgaagc agtcggcgca
     4201 tgatcttgcc ggaccgggtc ttgggcaggt catgggcaaa gagcacctct tccggccggg
     4261 ccagcgcgcc gatcttggtc accacgtgct ccttgagctc gtccaccagg tccggcgacg
     4321 gcttctggcc gcctttcagg ctgacaaagg ccacgatggc ctgccccttg atgtcgtggt
     4381 tccgcccgac cacggcggct tccgctacgg cggggtgatc caccagggcg gactccacct
     4441 ccatggtccc gatccggtgc gccgagacgt tcagcacgtc atcgaccctg cccaggatca
     4501 tgtagttccc cttggcgtcc ctcttggcgc cgtcccccgg gaagtagagc ttgtcggacc
     4561 atttcttgaa gtaggtggag cggaaccgat cgtcatcgcc ccagacggtg cgcagcatgg
     4621 agggccacgg gtgcgtgatg gccagcagcc cgcttccggt tttcacttcc ttgccggact
     4681 catccagtac cgtggccatg atcccgggga gcggggtgcc gacgaacccg ggcttcatgg
     4741 tggaaatgcc cgggagggtg gtgatcatga tcgcgccggt ctccgtctgc caccaggtgt
     4801 ccactacggg gcatcgaccg ccgccgatgg tctcgtgata ccacatccat gcctcggggt
     4861 tgatcggctc tccgaccgat cccaggagcc tgaggctggt gaggttgtgc ttccccggca
     4921 gctccttgcc ccacttcatg aaggtccgga tggccgtggg tgccgtgtag agcaccgaga
     4981 cctggtgctc ctcgatgagc tgccacgtgc ggtcccgctc ggggaaatca ggagctccct
     5041 cgtacatgag gaccgtggct ccgcagagaa gcggcccgta gaccacgtag ctgtggcccg
     5101 tgacccaacc gatgtcagcg gtgcaccaga acacgtcttc atcccgcagg tcgaagacca
     5161 ttcgcgtggt ggtctgcacc cccacggcat atccgcccac ggtgtggacg atgcctttgg
     5221 gcttgcccgt ggtgccggag gtgtagagga tgaacaggtt gtcctcggcg tccacgggga
     5281 tcgccgggca ctccaccttc tggtgatcca tgatgcggtg gtagaagtgg tcccttccct
     5341 ccatgatgtg caggaggtga ggctcgccgg gcagccgttt gacgatgacc acgtgctgca
     5401 ccgaggggca gcgctccagc gcgcggtccg cgttgtgctt gagctggacc acgttgccgc
     5461 ggcgccaccc gccgtccgcg gtgatgagca ccttggcctg ggagtcgatg atccggtcgg
     5521 ccagcgcctc gggagagaag cccccgaaga ccacgttgtg caccgcgccg atccgcgcgc
     5581 aggccagcat ggccaccgcg gactccagga gcatgggcat gtagattgcc acccggtcgc
     5641 ccttctgaac tcccagggcc ttcagacagt tggcgaactt gcagacctcc cgatggagct
     5701 gcaggtacgt gagggtgcga acctctccgt cctcgccttc ccagatgagg gcggccttgt
     5761 tccgccgcgg accgttcagg tggcggtcga tgcagttgac ggtgatgttg agctgcccgc
     5821 ccacgaacca tcgcgcgtgg ggcgggtccc actccatggc cttgtcccag gggcgggacc
     5881 actggagacg gctggcctcc tgggcccacc aggctaccgg gtcccgcgcg gcgtccgggt
     5941 agatgcgggc gtccctgacc tgggcatggg agctgaaatc ggacgggggg gtgaactccc
     6001 gttcctcacg cagcaggtca tcgatggccg tggtcttctc tgccatgccg tccccctcgt
     6061 cgaatgggat tcgcgttccg tctccggtat cgtagtggga atctaataac gccggccccg
     6121 cgggtcaaag ttgctctcca tcggcgggtc ggctatgttc tggacatgat ggttcgccgg
     6181 accatgctcg ccgcggggct cctggcgatc ggttctctgg cggcgggggc tgtcatcggc
     6241 ggcagatcct ccgcctgggc gcaggccatg ggcacgtttc caccgacaac caccgagtct
     6301 accggcgagc gggagctggg cgcgtttgtt caggcatacc cggatcgact gatcggcccg
     6361 gagctcagag acggggactg ggcggtgaag ctcggttctc gctggttcgc ttgggcgcac
     6421 ggccggatcc ttccggagga ccagcgctcc gcgtgggagt cctacgcggc gtatcggttc
     6481 tatcgatacc ccgtcggcgc tctgcctccg ttgccgcagc tcgacgccca gagcatcgcc
     6541 cgcctccagg ccatcgtgaa gcaatcccag gccaggcctc cgcgtcgatc cgaggcgttc
     6601 ctggagtcgc tcctgggcgc cgccaccctg gatcagatcc gcggcaacct ggtgaccgtc
     6661 ccgttgctgg gattccccgt tcagatccac tcggtcgcgg ccgccgcgct gcggaaggtg
     6721 gcggcggagt gcgcgctgct ggtgaagacc gaccctgagg ccgcggcctt ctttgccggg
     6781 atccatgaga tcgatggatt caactaccgc gaggtagccg gcacggcgac ccgcagctac
     6841 cacggctacg gcctggcgct cgacctcatc ccgcgaagct atggggggaa gccggtgtac
     6901 tggctctggg ccatgcaggc cggcgaatcc tggtgggcca ttccgtacga caggcgttgg
     6961 atggtgccgc tctccgtggt gacggccttc gagaacaacg ggtttgtctg gggcgggaag
     7021 tggatgttct tcgacatgat gcacttcgag taccggcccg agctcctttc actcgcgcga
     7081 gagggcgcgg cgccgtagcg cgggacaggt cgtccggctc ggccctctgc gcgtcagggg
     7141 ctatcccagg ctcgcgagat cctgtaggat gacgtcatca catcttggag ggggattgcc
     7201 catggctgat gaaccgaaag ccgcacgcaa aggaccgaga cggaggcgcc gcaagggagc
     7261 cgctcggaaa acggtcgcca agcgcggcca gaagatcacc ctgtcgattc cagatctcaa
     7321 caagctcacc aaggcagaga tacagttcat cgcgacctac aaggcttgag tccggaggcg
     7381 ggaggctgcg gtttcccgcc gctccttccg cctctcatcc ttgcggtctg gccccttcct
     7441 ccagtcggtt cgaatacttc ccgtagtaat gggcgccctt ccagcttcgc ttgagggccc
     7501 ggtcaaagcg gtgcacggcg gtgtagctga ccaggtttcg gatgccgacg aaggagcgca
     7561 gggaccgcca ctggcttctg gggaacgagt agatgtggtg cttgatgtag ttgtctccgc
     7621 ggtagatggt ttccgcgtcc acgcccaccg gtgaacgaac cacgtacgac aaccgatagc
     7681 tcgcccagtc ctgcgggaag ctcgtcctgg tgagcctgcc ctcggctttc atccgctcca
     7741 tcagcatgga tccggggaac ggcgtgagga tggcgagctg cacggcgtcc acgcccgcgg
     7801 agaccaggaa ccgggctatc tggcggtagt accgcaccga ctcgtggtcg ttcccgatga
     7861 tgaaggatcc gaccaccccg atgccggcgc gatggaagct gcggatcacg cggcgatagt
     7921 tgtccacgcc gatgcggagg ttgatcccct tcttcatgtc cttgagctga tcttcggcga
     7981 tgctctcgaa tccgatcagg gcaaagaggc acccggagcg gccggccagg cgcaggaggc
     8041 cctcatcctc ggcggagttg atggaggtct gcatccacca gcgcttcttg agcccgcggc
     8101 ggatcatgcc atcgaagagg gtccgggccc gctcgcggct c
//
